LOCUS       DAOSPL010000001        13317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_4677_length_13317_cov_5.961318, whole genome shotgun sequence.
ACCESSION   DAOSPL010000001 DAOSPL010000000
VERSION     DAOSPL010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 13317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13317
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4677_length_13317_cov_5.961318"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..744)
                     /locus_tag="PLQ54_00005"
     CDS             complement(94..744)
                     /locus_tag="PLQ54_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOS91668.1"
                     /translation="MPTRVFVVDDHKLLRELLRTLLENQPDYEVVGEAENGLEALQLI
                     PKTAPDLVLLDIMMPEMNGIEAARQILSTQPHIRILVLSMCTDKRLVSEAFRAGVSGY
                     LFKDCSTEEILRALRAITKGQAYLSPTIAGVVMADYVAGLSNEGSVWTLLTSREREVL
                     QLLAEGKRNKEIAALLGISEKTVETHRQHVQEKLGCESLADLIKYAIREGLTSLDR"
     gene            complement(801..3434)
                     /locus_tag="PLQ54_00010"
     CDS             complement(801..3434)
                     /locus_tag="PLQ54_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOS91669.1"
                     /translation="MSKDAQESRQTLAQLQEELRREIDLRQKAETKLRDREDQLRGYQ
                     QHLETLVAQRTLELRRTKEALEAQVAKRVEVQCLLERVIDTAPLALFWKDRDSRYIGC
                     NAAVARGMGLDSTADVMGRTDFELPWAAHAEQLRAEDRQVIESGQPLQGLERALTIGT
                     GPPVWFQVSKVPLMGLEGQVIGVLGCYEDISERKAHERRLIAQMDLARGLLDATSPEE
                     AARLCLVTTLQLSGMDCGGAYILEEDGGLRLICHQGVFDDFPRLVPYMAAGSPMAQAL
                     LRDEPSGFHFAETLPGPARMPMSREGIHWVGYMTVSHRGRMIGAICTASHDPTASPVH
                     ENLPALNAVAAMMGSTLVRFQAEEVARRSEQSLRESEARYRILFETVTDGMVIIDAAS
                     HRFLWANPAFCDMFGCSMDAIETMGMADIHPSEQLAEVYRGFAQFAEGHARTAQEIPC
                     LRQDGSAFPANISGASMELDGRRCWLGIFRDITEHKRDQEERRALEAQLFRTQRLESL
                     GVLAGGIAHDFNNILAGIQGFTELTHARLQNDSRAQRDLEQVLRATERAKDLVRQILT
                     FSRHVEQNRRPVELSLLLKETLKFLRASLPSTIRIEPHISPDGVVVQADPTHMDQVFV
                     NLCTNAQHAMQAEGGTLTVGLETVALSETEAHSLALAAPGSYAHLWVQDTGCGIPPAI
                     RGRIFDPFFTTKPPGEGTGMGLATAHGIVSGYGGTIHVDSEVGVGTVFHIYLPALDVE
                     TSEEYQLESALAEGTERILFVDDETSITLFVQELLESLGYRVTTRNESLEALRIFAED
                     PEAFDLVITDLTMPGLAGPELVARLRQMRPQIPVILMTGFPGFADGRSLEALGNPLLL
                     PKPCTAQELSDAVRRALDNRS"
     gene            complement(3848..6220)
                     /locus_tag="PLQ54_00015"
     CDS             complement(3848..6220)
                     /locus_tag="PLQ54_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOS91670.1"
                     /translation="MSLHALEVLLTRDDIPADVKEILRKEITDRTQLEALLEAMPDTI
                     YFKDTAGRFTRVNRAQAEVLGIASPDQALGKSDFDFFTPEHAWDAHADEQRIVTSGQA
                     VVNKVERIRRATGEFRWVSATKVPLRNGQGEITGIVGISRDIEDRVRAEEALRHAEWE
                     KVTILDSLPDLVVYQDKSHRILWANRAAADSVGLCREELMGRRCFEVWHASEKPCDPC
                     PIAAAMESKQIAGGSMNSADGRAWVIHGVPVLNAEGQVRGAIEVCRDVTRERAAEEAL
                     RASEARYRTIFDLSPQLIALLDIEGHIVDFNCRGQDWLGITREQAIGQSVFELSFFPE
                     ETRQVLRERWAQRLTGDLVVPYRVTFHDREDQERIGRVVGTLLRDENGEPTGALIMVA
                     DVTEQERLEAQVQHSQKLESLGVLAGGIAHDFNNLLMGVLGNASLALMQLSPEAPAYS
                     CVEHVQTAALRAAELSKQMLAYSGKGRFMVQPLNLSNLVEELTHLLESSISKKALLHK
                     RLPADLPAMAGDATQLRQIVMNLITNASDALGDDAGVISISTGTVHVDQAYLSSTYID
                     DRLPAGRYVYLEVSDTGCGMDAETQARMFDPFFTTKPTGRGLGMAAVLGIVRGHEGAL
                     KVYSEPGLGTTIKVLFRCIEEPAEALVLPPETMTVDNQVWTGTGSVLVADDEEVVRQV
                     ARATLELAGFTVIEAEDGQEALDIFRKHADEIVVVLLDLSMPRMGGKEALQAMRQLRP
                     DARIVLSSGFNEQDTISRLAGRRFSGFIQKPYKPTDLLATLRGVLDDRDS"
     gene            6584..7582
                     /locus_tag="PLQ54_00020"
     CDS             6584..7582
                     /locus_tag="PLQ54_00020"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOS91671.1"
                     /translation="MRKQAYDAALWILRLSGLMKREADRSRGRATILAYHGVTERPLG
                     ELDSPVLHLPLAVFRRHMALLADRFHVVPLMQLVRWIRAGETIPPRTVVITFDDGYRN
                     NITCALPVLRQLGLTACLFFTAGLVGTQEILWPDVIRRALRLLPLPFSAPLDGETVTV
                     DASNVRAVTRRLKQLPSEVREGFVAAVRDQLPRDLAPGESAIASAEEVRIWHQAGMEV
                     GSHSLTHPILSRLSGEALERELFESKRILEGIVGEPVRLLAYPNGRRADYSPAVLEVA
                     ERAGYEAAVTTLEDQVRAECPLLELPRHMPDESVLRVEAMASGWEARVQAAFRRGA"
     gene            complement(7607..9877)
                     /locus_tag="PLQ54_00025"
     CDS             complement(7607..9877)
                     /locus_tag="PLQ54_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOS91672.1"
                     /translation="MGELSRLSGLEVARVRTELTQASQRIAELQAGQQSLHQSRRQFP
                     RAVLSGMCSPAEANREIQRLVGEEARYARLYDEFSSARDQALIQGRTFATQLQQARAL
                     VGNSAHQGLALRQLGQRAQLANELGIRWSEAAVLTIQNAELAATAASELQRVVASAAQ
                     RHWGYREPIYVKPGTVSTGLADLLAVVSVALPVESSFASDLPQRAGLQVARGWLLDLL
                     ALAVTAGAGWGVLSLLSRVYRKRRQAPLGPWARLALIGARTALCLLAVWALIPALGER
                     DDPVQLAQLHRFYGLLAALGLATATLPALGRSISGLPAFSGADGAELRPAWCRAQQRL
                     STLALCSAIFVPGLISLREIGYPHEDVRGLLEGLFGVALAYGFSRLLAPGGLMAVLGL
                     APASSWAKKLALQSASLRQAVLLVSLVLLGLHGLGWTNLARTLAWGLSVSLLMVLGLG
                     VAIAVGPREVSGGWGRLTPWLLYALALAGAAWGWGLRGYHVGQFAGLLTATRFSFKNT
                     QVSGASLLQGIALAVALALLGRWLRRWIQCSTLTQRWDQGARYAVATTTYYICVTGGI
                     LAGVLAAGFQLSVLTVFAGMAGVAIGFGSQDIAKNFIGGVIILLDRSVNVGDYVIIGA
                     AEGTITDINIRCTTLRTPDGKVVIVPNANLIAQQMVNATQGHGGRIRLTVDVTVAMDS
                     DPDKVMAILTEVARQHPRVATEPAPRALVSRLSAAALNIQLIVWTAHPEDQTELAGEL
                     TTRIFRALREQKIVLA"
     gene            10363..11847
                     /locus_tag="PLQ54_00030"
     CDS             10363..11847
                     /locus_tag="PLQ54_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91673.1"
                     /translation="MAGLLDRVYPRMPVPVQDLMVSVRGYQLRRERFGGSFRHYCAEF
                     EATQWLPEAEHRQRQLEELRRLVAHAYENVPFYRERMKQAQVSPGDIDSLDALRLMPV
                     VDRQDIKAYTKDFIDRRLDPSRLLCIHTSGTTGACLDLYYTPEERRKAYALSQRSLAW
                     AGYQDGMRRATFNVRPIVPIARDRPPFSRVDWPSRTRLFSPFHLSPKNMPHYVREIRE
                     WGTQFIQAYPSHLWPVADYMVQEGIQGLKLAGIATSSETLVPKLRSTLEQAFSCRVMD
                     FYGMAEMAAFFSQCPEQTYHDFAEYAYVEFLDDADQPVKPGTPGRIVATSFITWSMPL
                     IRYNTRDLAVPSEDPEPCPCGRGLPKIRGLVGRTEDLVVTPDGRRLGRFTWLFKDTPE
                     IKQGQVIQKALDRILIRVLPAGEFPPELSEKLRAKCVKSLGDQVSVEVEVVSELARTA
                     SGKFRAVVSELPKNDLNWNAYGEITWDEEAVVGGGEGPSAPGNR"
     gene            complement(11961..13184)
                     /locus_tag="PLQ54_00035"
     CDS             complement(11961..13184)
                     /locus_tag="PLQ54_00035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS91674.1"
                     /translation="MPDWILPAIFGLSVFCCVYPYTVHPVLSWLILKLAPSRTAVTEE
                     FDESQLPTVTVIVPMHNEAASALTKIQNTLENQYPDHLIHLIVVADHCTDDTEEQIAR
                     CGDPRVSMIRSEGGNGKNHCLNQAVPMAQGDIIVMTDASCALGPQAIRNMIRPFAKPE
                     IGLVSIPKYQVGTGQGGQGGTLSKLRNAFLGFDSISKAAESKLGCLVGGIGALIAMRR
                     SLWEPVPDDVCNDLQIPLRLIAQGHQVVTAQDVECLLGPSKSLGKEMARRTRIISRSI
                     IPATWALGQYLKRGRLAALFFLAGRKLAFYFAPVFMILALVSNALLAPSGPIWLGLLV
                     LQVGLYLLAFLGIKLSERDSPLAKLLGIPGQFLALNLGVLTGLWYVLRHGHGMVTWST
                     AQRAIPGAEQDPAAG"
BASE COUNT         2325 a         4231 c         4382 g         2379 t
ORIGIN      
        1 tgcggcccct ccgaaagacc aggcgggcgt tccgcgcgac cttcgcgaga cccaggcgat
       61 agcctggtgg gactctatcc ggcccgaacg gcttcagcga tccagcgacg tcaggccctc
      121 gcgaatggcg tacttgatga gatccgccag gctctcacag cccagtttct cctgaacgtg
      181 ctgccgatgg gtctcgacgg tcttctcgct gatgcccagc agagcggcga tctccttgtt
      241 gcgtttcccc tcggccaaga gctggagcac ctcgcgctcg cggctggtca gcagagtcca
      301 gaccgacccc tcgttggaga gccctgccac atagtcggcc atcacgacgc cggcgatggt
      361 gggcgagagg taggcttgtc ccttggtgat ggcacgaagg gctctgagga tttcctcggt
      421 cgagcagtcc ttgaacaggt agccggatac tccggcccgg aaggcctccg acacgagacg
      481 tttatccgta cacatcgaca ggacgaggat tcggatatgg ggttgggtgg acaggatctg
      541 acgcgccgct tcgatgccgt tcatttcggg catcatgatg tccaagagca ccagatcggg
      601 cgcggttttg gggatgagtt ggagcgcctc gagaccgttc tcggcttctc ccacaacctc
      661 gtagtccggc tggttctcca acagggtacg aagcagctcc cgcaatagct tgtggtcatc
      721 taccacgaac acccgcgtcg gcatggggac ctgctttcct cccggggcca tggggcgtgg
      781 acgccccggt ttcttggatc ctacgatcgg ttatcgagcg cacggcgcac cgcatcgctg
      841 agctcctgag ccgtacaggg cttggggagc agcaacgggt ttcccagcgc ctccagactc
      901 cttccgtcgg caaatcccgg gaagcccgtc atcaggatca ccgggatctg gggccgcatc
      961 tggcgcaaac gagcgaccaa ctccggtccg gccaacccgg gcatggtcaa atcggtgatg
     1021 accagatcga aggcctcggg gtcctccgcg aagatccgca gcgcttcgag gctctcattc
     1081 ctcgtcgtga cccggtatcc caaagactcg agcaattcct ggacaaatag ggtgatcgaa
     1141 gtttcgtcat cgacgaacag aatccgttcc gtgccctcgg ccaaagccga ctccagctgg
     1201 tactcttccg aggtttccac atcgagggcc ggcaagtaga tgtggaagac ggtgcccacc
     1261 ccgacctcgc tgtcgacatg gatggttcca ccatatccgg agacaatgcc gtgagcggtg
     1321 gcgagtccca tgcccgtccc ctctcccggg ggtttggttg tgaagaaagg atcgaagatc
     1381 cggccgcgaa tggcgggagg gataccacac cccgtgtcct gcacccagag atgagcatag
     1441 gacccggggg ccgccagggc gagggagtgg gcttcggtct cggacaacgc cacggtctcc
     1501 agccccacgg tgagggtgcc gccctcggcc tgcatcgcat gctgggcatt ggtgcatagg
     1561 ttgacgaaga cctgatccat gtgggtggga tcggcctgca ccaccacgcc gtccggcgag
     1621 atgtgcggct cgatccggat ggtgctgggg agagaggctc ggaggaactt cagggtctct
     1681 ttgaggagca acgatagctc aacgggccgc cggttctgct ccacgtgccg gctgaaggtg
     1741 aggatctgtc ggaccaggtc cttggcgcgc tcggtggcgc gaagcacttg ctccaggtca
     1801 cgctgagccc gcgagtcatt ctgaagccgg gcgtgagtca gctccgtgaa tccctgaatg
     1861 ccggccagaa tgttgttgaa gtcgtgggcg attccgccgg ccaggacccc aaggctctcg
     1921 agccgctggg tgcgaaagag ctgggcttcg agggcgcgcc gttcctcttg atcccgcttg
     1981 tgttcagtga tgtcgcggaa gatgcccagc cagcatcgcc gcccatccag ctccatggac
     2041 gcccctgaga tgttggcggg gaatgccgat ccgtcttggc gcaagcatgg gatctcctgg
     2101 gcggtacggg catgcccctc ggcgaactga gcgaagccgc gatagacttc cgccagctgc
     2161 tcggaggggt gaatgtcggc catccccatg gtctcgatcg catccatgga acagccgaac
     2221 atgtcgcaga atgcggggtt cgcccacagg aatcggtgcg aggccgcgtc gataatgacc
     2281 atgccatcgg tgacggtctc gaagaggatg cgatatcgcg cttccgactc tcgaagcgac
     2341 tgctcgctcc gccgggccac ctcttcggcc tggaagcgca ccagagtgct gcccatcatg
     2401 gcggccacgg cgttcagggc gggcagattc tcgtgcacgg gggaggcggt ggggtcatgc
     2461 gaggcggtac agatcgcgcc aatcatccgc ccgcgatggg aaacggtcat atagcctacc
     2521 cagtgaatac cctcccgaga catgggcatt cgggccgggc cgggcagggt ctctgcgaag
     2581 tggaaaccgg atggctcgtc tcggagaagc gcttgggcca tcggactacc ggcggccatg
     2641 tagggcacca gccgagggaa gtcatcgaac acgccctggt ggcagatcag ccgtagcccg
     2701 ccgtcctctt cgagtatgta tgcgccgcca caatccatac ccgagagctg gagtgtggtc
     2761 acgagacaca accgtgccgc ttcttccggc gaggtcgcat cgaggaggcc gcgggccagg
     2821 tccatctggg cgatcagtcg gcgctcgtga gctttgcgct cgctgatatc ctcatagcag
     2881 ccgagaacgc cgataacctg gccctcgagg cccatcagcg gaactttgct cacctgaaac
     2941 cagaccgggg ggccggtccc gatggtcaga gcccgctcca ggccctgcag gggctggccc
     3001 gactcgatga cctggcggtc ttcggctcgg agttgctcgg cgtgggccgc ccaggggagc
     3061 tcgaaatccg tccggcccat cacatcggcg gtcgagtcga ggcccatccc ccgggctacc
     3121 gccgcgttgc agccgatgta gcggctatcg cggtctttcc agaacagggc cagcggggcc
     3181 gtatcgatca ctcgttccaa caggcattgg acctcgaccc gcttggcaac ctgggcttcg
     3241 agagcctcct tggtccgccg gagttcgagg gtgcgctggg ccaccaaggt ctcgaggtgc
     3301 tgctgatagc cacggagctg gtcttcgcgg tcacggagct tggtttcggc cttctggcgc
     3361 aagtcgattt cccggcgcag ctcttcttgg agctgcgcca gtgtttgccg agattcctgc
     3421 gcatccttac tcatacaccc cagattccct ccccaacggg cacacaacct ccgggcagcc
     3481 gggcctcgcg agaaaaacgg cccgggttcg tcggctgtgt ccccaacggg tacgaagccg
     3541 tggaagagta accccggaag cgctccgtgg tggcgttggg gctggatgag cgcttccacc
     3601 atcttccgcc acgcgcgttg agcctggggc ggcaggtgga tccgtggtgg tttgtgggga
     3661 tgcggagagg tggcgctgcg gttgatctga agcactcagt ggtggtggcg tggtggtgga
     3721 gtgcttcagg cgatgatgca ccgtttctca caattgattc agcctacgga accggtccgg
     3781 gcggagcggt tctgtaggcg tttgcgtgcg gagaacccga tgacgccacc agggcccagg
     3841 gcgggcatca ggagtcccga tcgtccagca caccgcgcaa ggtggccagg aggtcggtgg
     3901 gtttgtaggg tttctggatg aatccggaga agcgccgtcc cgccaaccgg ctgatggtat
     3961 cctgctcgtt gaagccgctc gagagcacga ttcgagcatc gggccgcagt tgccgcatgg
     4021 cctgcaaggc ttctttgccc cccatgcgag gcatgctgag atccagtaag accacgacga
     4081 tctcatcggc gtgtttgcgg aagatatcga gggcttcctg accatcctcc gcctcgatta
     4141 cggtaaaccc ggccagttcg agggtggccc gggctacttg gcggacgact tcctcatcat
     4201 cggccacaag aacgctgccc gtccccgtcc aaacttgatt gtcgaccgtc atggtctcgg
     4261 gggggagtac cagggcctcc gcgggctcct cgatgcagcg gaagagcacc ttaatggtgg
     4321 ttccgagccc tggctcgctg tagaccttga gcgccccttc gtgcccgcgg acgatgccaa
     4381 gcacggcggc catacccaag ccgcgcccgg tgggtttggt cgtgaagaag ggatcgaaca
     4441 tgcgtgcctg ggtctccgca tccatcccac agcccgtatc cgagacctcc aggtagacat
     4501 accgtccggc agggagccgg tcgtcgatgt aggtgctaga caggtaggcc tgatcgacgt
     4561 gaaccgtgcc ggtactgatg gagatgaccc cggcgtcatc gcccagcgca tcggaggcgt
     4621 tcgtgatgag gttcatcacg atctgacgca gctgagtggc atcgccggcc atggcgggaa
     4681 gatccgcggg cagccgtttg tgaagcaggg ctttcttgga gatggaagac tcgaggagat
     4741 gggtcagttc ctccaccagg ttcgatagat tgaggggctg aaccatgaac cgccctttgc
     4801 cggaataggc cagcatctgc ttggagagtt cggccgcgcg cagtgccgcc gtctggacat
     4861 gctcgacaca tgagtaggcg ggtgcctcgg gggagagctg catcagggcc agggacgcgt
     4921 tccccagcac tcccatgagc aggttgttga agtcatgagc gatacctccg gccaacaccc
     4981 cgaggctctc gagtttctgt gaatgctgaa cctgggcctc gagccgctct tgttcggtga
     5041 catcggccac catgatgagg gcaccggtcg gctcgccatt ctcatcgcgg agcagggttc
     5101 cgaccactcg gccaatgcgc tcctggtctt cccggtcgtg gaaggtgacc cgatagggca
     5161 cgaccagatc gcccgtcagc cgttgcgccc atcgctcccg aagcacctgg cgggtttctt
     5221 cgggaaagaa ggacagctcg aagacacttt ggccgatggc ctgttcgcgg gtgatcccaa
     5281 gccaatcttg ccctcggcag ttgaaatcaa cgatgtgccc ttcgatgtcc agcaaggcga
     5341 tgagctgggg ggaaaggtcg aagatggtgc ggtatcgggc ctcgctggct cgcagggctt
     5401 cctcggccgc ccgctcccgc gtcacatcgc ggcacacctc gatagccccg cgcacttgcc
     5461 cttcggcgtt caggaccgga acgccatgga tcacccaggc acggccgtcg gcggagttca
     5521 tgctgccccc ggcgatctgt ttcgactcca tggccgccgc gatggggcag ggatcacagg
     5581 gcttctccga cgcgtgccag acctcgaagc agcggcgccc catcagctcc tcgcgacaga
     5641 ggcccaccga gtcagccgcg gcccggttgg cccataggat gcggtgggac ttatcttgat
     5701 agaccaccag atcgggaagg ctatcgagga tcgtgacctt ctcccactcg gcatggcgca
     5761 gggcttcttc agcacggacc cgatcctcga tatcgcggct gataccgaca atgcccgtga
     5821 tctcgccctg gccgttacgg aggggtactt tggtggccga gacccaacgg aactcaccgg
     5881 tggcccgccg gatacgctcc accttgttca cgaccgcctg gcccgaggtg acaatgcgct
     5941 gctcgtcggc atgggcatcc caggcgtgct cgggtgtgaa aaagtcgaag tcgctcttac
     6001 ccagagcctg atcgggactg gcgatcccca atacttcggc ttgcgcgcgg ttcacccggg
     6061 tgaaccggcc cgccgtgtcc ttgaagtaga tggtgtcggg catggcctcg agcaaggcct
     6121 cgagctgagt gcggtcggtg atctctttgc ggaggatctc tttgacatcg gccgggatat
     6181 cgtcgcgtgt cagtagaacc tctagcgcgt gcaggctcat ggttcagctc cgcatcgtgc
     6241 attggtcgat ccagccctgc gcccaggacg ggctcttgtc gtgcattctt cctgacggga
     6301 ccggatacgt aactttgacg ccccagaagc acccatcgtc acaccgtata ttgtctgttg
     6361 tcactctcac cccttgcggc cccatcgctt cttggggcgc tatccgcgaa gaccgcggtg
     6421 ggtgagactt cagccgcacg cgaagacact ccacaagcca ttgtaagcct gggcgaaaac
     6481 catctggggc tgaagttggc tacagtatct gttatgcccg atgatcccgg atgcttccgt
     6541 gcgggccttc ggtaatgatc accggttttc cggggagttg accatgcgga aacaggccta
     6601 tgacgcagcc ctgtggatac ttcgcctctc gggtctcatg aagcgtgaag cggatcgctc
     6661 gcgcggccgt gccacgatcc tcgcttacca cggcgtaact gagcgtcctc tcggggaact
     6721 cgattcaccg gtcctgcatc tgcctctcgc cgtcttccgc aggcacatgg cgcttttggc
     6781 agaccgcttc cacgtggtcc cactgatgca gttggtccgt tggatccgcg cgggcgaaac
     6841 cattccgccg cgcacggtgg tcatcacctt cgacgacggc taccgcaata acatcacgtg
     6901 cgcccttccc gtgctccggc aactggggtt gaccgcgtgt ttgttcttca ccgccggctt
     6961 ggtcggcacc caggagattc tgtggccgga tgtgattcgg cgcgccctgc gactgcttcc
     7021 cctccccttc tccgccccgc tggatgggga aacggtgacc gtggatgcct ccaatgtccg
     7081 agcggtcacg cgccggctga agcagcttcc cagtgaagtg cgtgagggtt tcgtggcggc
     7141 agtgcgcgat cagcttcccc gcgacctggc tcccggtgag tctgccatcg cctcggctga
     7201 agaggtgcgg atctggcacc aggcgggcat ggaagtgggc tcccattccc tgacccaccc
     7261 cattctgagt cgcttgtcag gtgaggccct cgaacgggag ctattcgagt ccaagcggat
     7321 tctggagggt atcgtgggag agcccgttcg gctgttggcc tacccgaatg gccggcgggc
     7381 cgactacagc cctgccgtgc tcgaagtggc cgagcgtgcc ggctatgaag cggcggtgac
     7441 caccctggag gatcaggttc gcgccgagtg ccccctgctg gagcttccgc ggcacatgcc
     7501 cgacgaatcc gtgctgcggg tggaagccat ggcatccggc tgggaggcgc gggttcaggc
     7561 ggcgttcagg cgaggggcgt gatgctcgag agccgggctg gtcctctcac gcgagcacga
     7621 tcttctgttc ccgaagcgcc cggaagatcc gggtcgtgag ttcgccggcc agctctgtct
     7681 gatcttcagg gtgagcggtc cacacgatca gctggatgtt cagcgcggcc gcggagagtc
     7741 gtgacaccag cgcgcgaggg gcgggctccg tggcgacccg aggatgctga cgcgccacct
     7801 cggtcaggat ggccatgacc ttatcgggat cgctgtccat agccacggtg acatccacag
     7861 taaggcggat acgcccgcca tgtccttggg tggcgttgac catctgctgg gcgatgaggt
     7921 tggcgttggg gacgatgacc accttgccat cgggagttcg gagcgtggtg cagcggatgt
     7981 tgatatccgt gatggtcccc tcggccgcgc cgatgatcac gtagtcgccc acattgacgg
     8041 accgatcgag caggatgatg acaccgccga tgaagttctt ggcgatgtcc tggctcccga
     8101 acccgatggc cacaccggcc attcccgcga acacggtgag cacggagagc tggaaacccg
     8161 cagccagcac gcccgccaga atgccacctg tcacgcatat gtagtaggtc gtggtggcta
     8221 cggcatagcg cgccccctgg tcccatcgct gtgtcagggt agagcattgg atccaccggc
     8281 gcagccagcg ccccagcagg gcgagcgcca ctgccagggc tattccctgg agcagtgatg
     8341 cgccggacac ctgggtgttc ttgaaggaga accgggtcgc ggtgagcagg ccggcgaact
     8401 ggccgacgtg atagccgcgg agtccccaac cccaggccgc acccgccaga gcgagagcgt
     8461 acaagagcca gggggtcagc cgcccccaac ctcccgaaac ctctctcggc cccaccgcga
     8521 ttgcgactcc cagccccaag accatgagga ggctgaccga tagcccccat gccaacgttc
     8581 gggcgaggtt ggtccagccc agcccatgga gtcccagcag caccaggctc accagaagga
     8641 ccgcctgacg cagcgaggcg gactggagcg ccagcttttt ggcccagctc gaggccggcg
     8701 ccaggcccaa tacggccatc agccctcccg gcgcgaggag gcgggagaag ccataggcga
     8761 gtgccacgcc aaagaggcct tcgagcagtc cgcgtacgtc ctcgtgcggg taaccgatct
     8821 cccgaaggct gatcagaccg ggcacgaaga tggcggagca gagggccagg gtcgagagac
     8881 gctgctgggc tcggcaccag gcggggcgaa gctcagctcc atcggccccg gagaatgcgg
     8941 ggagaccgga tatggagcgc cccagggcag gaagcgtggc agtggcgagc cccagggcgg
     9001 ccaggagacc gtagaaccgg tggagttgcg cgagctggac cgggtcatct cgctctccca
     9061 gggccggaat cagcgcccag acggccagca ggcagagggc cgttcgcgct ccgatcagag
     9121 ccaggcgagc ccacgggccc aagggagcct gacggcgctt ccggtaaacg cgggatagga
     9181 gggagaggac tccccaaccg gcccctgcgg tgaccgccag tgccaggaga tccagcagcc
     9241 agccccgagc cacctggagc ccggcccgct gggggagatc ggaagcgaat gacgactcga
     9301 ccggcagcgc gacgctcacc acggctagca ggtcggccaa acccgtcgat accgtgcccg
     9361 gcttgacata gatgggctct ctatagcccc agtgtctctg ggcagccgag gccacgaccc
     9421 gctggagctc cgacgcggcg gtggcggcca gctcggcatt ctggatggtc agaacagccg
     9481 cctcgctcca tcggattccc aactcgttgg ccagttgggc ccgctggccg agttgacgca
     9541 gagcaaggcc ctggtgggcg ctattcccta cgagcgcccg ggcttgctgg agttgcgtcg
     9601 cgaaggtccg tccttggatg agagcctggt cgcgggcgga tgagaactcg tcatacaacc
     9661 gggcgtagcg ggcctcttcc ccgacgagtc gttggatctc ccgattcgcc tccgccgggg
     9721 agcacattcc cgagagcacg gcccgtggga actggcggcg gctctggtgc agactctgtt
     9781 ggccggcctg cagttcggca atccgctggg aagcctgagt cagctcggtg cggacgcgcg
     9841 cgacttcgag acccgagagg cgagagagct cgcccatcca tccggtatcc gacggtgctt
     9901 gttccgctcg gtcaagccag gactggattc ggtccggggc ggggggggat ggtgcagcgg
     9961 cagcggcaag gactcccgcg gcccatagcc agcccatgag cgtcccgtgc caccgaacac
    10021 catgacgcac gctgtgccac atggcgcgat tgtagcagtc cgcctcgggg gacgaaaccg
    10081 aggagacttg cgccctttcc ccccgtctca gcctcggatt ccatctggca taatggaaca
    10141 gtcttccggg gaaagagggg accgaactca agaccccgag ggcctagcgc gcctcaccag
    10201 gctcccgtag gcgaggcttt ggcctcgtgc tcacccgatc cggaggccct gtgatccgag
    10261 gctgaagcct cggctactga accaagagag tagccattgg ggtccaagat cctgggtgtg
    10321 ggcctttggg gatcaggccc tgtgccccga tgggaggaac ccgtggctgg cttactcgat
    10381 cgcgtgtacc cgcgtatgcc ggtgcccgta caggacctga tggtaagcgt acggggctat
    10441 caactccgcc gagagcgttt cgggggcagt ttccgacact actgcgcgga gttcgaggcc
    10501 acccagtggc tcccggaagc cgagcatcga cagcgccagc tcgaggagct ccgtcggctg
    10561 gttgctcacg cctatgagaa tgtgcccttc tatcgggagc gcatgaagca ggcacaggtg
    10621 tcgccgggcg atatcgattc cctcgacgcc ctccggctca tgcccgtggt ggaccggcag
    10681 gacatcaagg cctacacgaa ggacttcatc gaccggcgcc tcgatccgag ccgcctgctg
    10741 tgcatccaca ccagcgggac caccggggcg tgcctcgacc tgtattacac gcctgaagag
    10801 cgccggaagg cctatgcact ctcgcagcgg tcccttgcgt gggccggcta tcaggacggc
    10861 atgcgccggg ctacgttcaa tgtccggccc atcgttccga tcgctcgcga tcggccgccg
    10921 ttctcgcggg tggactggcc gtcgcgaact cggctgttct cgccctttca tctgtcccct
    10981 aagaacatgc cccactatgt gcgggaaatc cgcgagtggg gtactcagtt catccaggct
    11041 tacccctcgc atctgtggcc cgtggccgat tacatggtcc aggagggcat tcaggggctg
    11101 aagctggccg gtatcgccac ctcttccgaa acgctggtgc cgaagctgcg gtccacgctg
    11161 gagcaggcct tcagttgccg ggtgatggac ttctatggca tggccgagat ggcggccttc
    11221 ttcagccagt gccctgagca gacctatcac gacttcgccg agtatgccta tgtggagttc
    11281 ctcgacgatg cggatcagcc ggtgaagccc ggcacgccgg gacgcatcgt ggccacgagc
    11341 ttcatcacgt ggagcatgcc cctcattcgc tacaacaccc gcgacctggc ggtgccctcg
    11401 gaggacccgg agccgtgccc ctgtggccgc gggctgccca agatccgcgg gctcgtgggg
    11461 cgcaccgagg atctggtggt gactccggat ggtcgccggc tcggccggtt cacatggctg
    11521 ttcaaagaca cgccggaaat caagcagggc caggtcatcc agaaggcgct ggatcggatc
    11581 ctgatccgcg tgctgccggc cggcgaattc ccacccgagc tctcagagaa gctccgagcc
    11641 aagtgcgtga agtctctcgg cgaccaggtc tcggtggagg tcgaagtagt ctccgagctg
    11701 gcccgcacgg cttcggggaa gttcagggcc gtggtctccg agctgcccaa gaacgacctg
    11761 aactggaacg cctatggcga gatcacgtgg gacgaagagg ccgtcgtggg cgggggggaa
    11821 ggaccctctg cgcccgggaa ccggtgatcg gcgcgtggcg ggatagggcc actgctgcct
    11881 acaagcctat gcggaggcct gaccgacttg gttgccgtcc cgggcaacga cccgtccgtc
    11941 cccgctcgtt ccgcccgccc ctaccccgcc gccgggtcct gctccgctcc ggggatggcg
    12001 cgctgagccg tggaccaggt gaccataccg tgaccgtgcc ggagcacgta ccacaggccg
    12061 gtgagaacgc ccagattcag cgccaggaac tggccgggga tgcccaggag cttggccagc
    12121 gggctgtcgc gctccgatag cttgatgccg aggaaggcca gcaggtacag gcccacctgg
    12181 agcaccagga gacccagcca gatcgggccc gagggcgcca acagcgcgtt cgagaccaaa
    12241 gccaggatca tgaacacggg cgcgaagtag aacgcgagct tccggcccgc gaggaagaac
    12301 agggccgcca ggcgcccgcg cttgagatac tgtcccaggg cccaggtggc cgggatgatg
    12361 ctacgggaga tgatccgcgt gcggcgcgcc atctccttgc ccaggctctt cgacgggccc
    12421 agcaggcact ccacatcttg agcggtcacc acctggtggc cttgagcaat cagccgcaag
    12481 gggatctgca ggtcgttgca gacatcatcg ggcaccggct cccacaggga ccgacgcatg
    12541 gcgatcagtg ccccgatgcc tcccacgaga cagcccagct tcgactccgc cgccttggag
    12601 atgctgtcga agccaaggaa cgcgtttcgt agcttcgata gcgtgccccc ctggccgccc
    12661 tggccggtgc ccacctggta cttggggatc gagacgagcc cgatctcggg cttcgcaaag
    12721 ggccggatca tgttgcggat ggcctgggga cccagggcgc aactggcgtc ggtcatgacg
    12781 atgatgtcac cttgggccat gggcacggcc tggttgaggc agtggttttt cccgttgccc
    12841 ccctcagacc ggatcatcga aacccgcgga tcgccgcagc gcgcgatctg ctcctcggtg
    12901 tcatcggtgc agtggtcggc gaccacgatg aggtggatca ggtggtccgg gtactggttc
    12961 tcgagggtgt tctggatctt ggtcagcgcc gaggctgcct cgttgtgcat gggcacgatc
    13021 acggtgaccg tggggagctg ggactcgtcg aactcctccg tcacggccgt gcgcgagggg
    13081 gccagcttca gaatgagcca ggagaggacc gggtgcaccg tgtaggggta cacgcagcag
    13141 aacacgctga ggccgaagat cgccggcagg atccagtcgg gcatgagtcc gcgtcgtttc
    13201 ccttccccaa agctctcggg ccggatcggg tcccaccagc ctgcggctgg ttccccctat
    13261 tcggcattgg cggtaactta gggctccagc ggcccatggg cctggttgcg gaggggc
//
LOCUS       DAOSPL010000002        11507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_5597_length_11507_cov_3.321603, whole genome shotgun sequence.
ACCESSION   DAOSPL010000002 DAOSPL010000000
VERSION     DAOSPL010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 11507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11507
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5597_length_11507_cov_3.321603"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PLQ54_00040"
     CDS             <1..524
                     /locus_tag="PLQ54_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS91675.1"
                     /translation="ARVGIPRVVSHIGFVPEDDRRTHDAVRDAVREVCDYMGERSLVF
                     ALETGQEKPEVLRDFIEQVNRPNLRVNFDMANLILYGSGWPLPALDVLMDYVDGTHCK
                     DGTWPTEPGRLGKEQPLGKGQVNVEAVIQRLYDGGYRGPLDIEREIEGEQQRLDIMEA
                     IELLRGIRAGLGL"
     gene            726..2852
                     /locus_tag="PLQ54_00045"
     CDS             726..2852
                     /locus_tag="PLQ54_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91676.1"
                     /translation="MADVHALLTNAWRLDLAGNCLSVMPRRPRPGFVGSVGYIHPVDW
                     PGLFEVEVTCAGAPVQWEPASHTWGPAGMRAEYAGGGLALREAKTITRHDVAACQLIV
                     TRTAAESAGIEVWIRPLLPGAEDLRAEGDTLFGTVAVHLRPSGLSPMDFVKHWALRCP
                     GAEALAEPAGVRRSATLGPGESLTLTVALALRDEPGACLIPIGSMVGLSDPVAANAER
                     ENAWYADRVPPFACSDPLITRLYYYRWYLARRNTVDARAGFAGHPYDRPCVFESRSGR
                     ASARLSPHALAQQLVDLSWMRVPDVAQDLAWVVLRQGQVLAEASGGRTPDPPDWTPMA
                     LWRLMLVHPDPALLAAAYPEACRLVRGWASESHDVDRDGRPEVRGSFAMGLEYLPSFF
                     YFTQPRWDHTQGEEFGRAAGLERPDTQGDFYLSAMACAAMAVALGREAEAREWQSLAD
                     RAKSAALEVMWDAGIGFFCPVEPRTHERAPVKEATGFVPFIAGLAGPEHVVATAALSD
                     PNQFGTPFPVPSVSMDCEAFSPSDTWIAGPNASPQRPHMYRRAHNGPSWPQLTSLVAD
                     AAACACRLAPGDDTPRMAFAELFRKYTRMHFRGDDPPTPCVVDEYHPLTGEWLGDSMD
                     CFASHYIDLVVRHVAGLQPRADGVLEIWPIDIGLQAFSLQDVPYQGHVISIMWERGGP
                     GLIVTMDGKAVARQPGLSPPITVQMR"
     gene            2871..3623
                     /locus_tag="PLQ54_00050"
     CDS             2871..3623
                     /locus_tag="PLQ54_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOS91677.1"
                     /translation="MGLYEQDAQRGLREPGIGRLARVAIVENGDPMVRLDSERLGRRV
                     FIQAKLPWVRVAVADMLTQALASLPEPLTLRISTAYRTLQMQRRGYERYMRQLAREHP
                     EWPRAILVRQSNRFFHPVTSKAPPGHCTGGAVDVALVRSRTGTLLDHTSTTVSVPNSW
                     PTFCPVLTDTARANRAVLYEAMRAAGFSNCFDEWWHWSYGDSGWAARLGHRHAVYGLV
                     TEYPPEMEEEILRKRPSGIRRRDIRPSLEGNR"
     gene            complement(3661..5115)
                     /locus_tag="PLQ54_00055"
     CDS             complement(3661..5115)
                     /locus_tag="PLQ54_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOS91678.1"
                     /translation="MARGLVPFALVAALAGGPAADAAKVIIITPHWEGIRYEYGRAFE
                     AWYEARYGEKVTVEWLDQGGTSDDVKFIRSEFAGGKDSIGIDLFWGGGIDPHIEFAKE
                     GLYVPYRIDPAVLAAMPPTYCGVPMYDADLLWYGQALSGFGILVNHRVLRQMGLPTPR
                     TWEDLGRPELAGWVGSADPRSSGSIHMMYEIILQAYGWDKGFQVIHRLGANIRAFPNS
                     SSQTPKDVALGEAAVGMAIDQYALAQVAESGSDVMEFVMPTGQTVVNPDGIGILKGAP
                     NRRVAERFIDYVMSTEGQMLLLLEPGTPGGPERFALGRMSVRPDVYEKVGPKTLTKVN
                     PFTLPSSLRYDPKRGGLRWALVNDLIGALVIDAHDELQAAWAACRRAGMPPDVLERMC
                     AMPVTEDEATRLATDVWKKADAKNVEIDRWTRFARAKYAQVLEMTGGPPRFQWLARTV
                     RYTLPVALAIGMLALFAIDVLRRFGVAVALRRAG"
     gene            5322..6347
                     /locus_tag="PLQ54_00060"
     CDS             5322..6347
                     /locus_tag="PLQ54_00060"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOS91679.1"
                     /translation="MKIGIIGPEGSGKSTVFACLTGLGVPAATRSPSLGVVRVPDNRL
                     EVLSAMYQPKKTTPAEITFVDLGSAGRHERLSAITGCLADADALAIVVGGLSPDSPGV
                     ALERFMLDLILADLSVVENRLERLEKDWQRGRKEAAEERDLMGRLQAALSEGRRIAEV
                     PLDEADEARLRGFQLTTRKPTVVLANVSEQDLGNGVAQSVAAIAEPRALRTFELCAPL
                     EAEIAELAHDERAAFLADYGLECAATDRFIRAAYEMLDLISFFTVGPDEVRAWSVRRG
                     TLAPRAAGRIHSDIERGFIRAEVVAYEHLSTAGSMAACRAQGQVRLEGREYEVRDGDV
                     IEFRFSV"
     gene            6441..7256
                     /locus_tag="PLQ54_00065"
     CDS             6441..7256
                     /locus_tag="PLQ54_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS91680.1"
                     /translation="MKLAICNEMFEKRPLGEVIDFVAQLGYDGIELAPFTLAGHADDV
                     LPATRAQIRKHASDAGISVVGLHWLLVTPAGLHIAARDPQVRKQTIKYLKSLATLCAD
                     LGGKIMVFGSPKQRNVPEGESYDENWLLAVDTIRQAGQFCQGLGVVIAMEPLRSDLTN
                     FLQTAAECRRFVDEVALPSVRMTLDCYSMAPMEESIPAALRAQADVVSHVHLNDDTGR
                     EPGTGSVDFVAVGQALRDIGYQGYASIEVFEFKPDAETIATRGLAHIRQAFGL"
     gene            7356..8630
                     /locus_tag="PLQ54_00070"
     CDS             7356..8630
                     /locus_tag="PLQ54_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS91681.1"
                     /translation="MHKKDSMTRRDFLGKTATVAAGVSAFALASPRALGANERIRVGM
                     LGPGGRGRYLMDRVNQIDNVEFVAVADIFEGWKNLGLEIARRKNPRAKGYDLLERMLE
                     DKDVDAVVAATPEHTHVDHIINTIKSGRDIYCEKPMNHTWEEGIRVIEANKKAKRIIQ
                     IGTQRRSVDLYYQARELVQSGAIGRITAVRGRWHRNSKDNDPQWRYAIPPDASEKNIN
                     WPVFLGKAPKVPFSLNRYFQWRCYWDYSNGPAGDLMVHQLDAINLVMGGQMPKSAMGL
                     GDIYRFHELGRTTPDTWSSILEYPGGSPWAPDGYLVQYSCVFSNQQDQYGEIFYGTDG
                     TIDMNDRVLRLIPEPEGVAMKKVEAKEFQTQVPDHGKAHLENFFDCCRTRKQPNCDEY
                     QGHYAASAAHMAVLCHFSGERTLWDDKKQRVE"
     gene            8712..9374
                     /locus_tag="PLQ54_00075"
     CDS             8712..9374
                     /locus_tag="PLQ54_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOS91682.1"
                     /translation="MSAIEMESWGGIAKALVSLVAVVNPLGSIPVFLSVTAERTPDER
                     RRMARITAISVGITLIVSALVGEQVLRMLGITLPAFRVAAGVLVLLMSLHMVQASPSR
                     TQHTPEEAAEAEDREGAAIVPLAIPLLSGPGSMSTVVLYSQGCRRWQEMAVLVALCAA
                     IGVLVWFVLSAAARLRARLGQTGIRVLTRIMGVFLAAIAVEFITSGLGDLLPGLVTVK
                     GP"
     gene            complement(9388..>11507)
                     /locus_tag="PLQ54_00080"
     CDS             complement(9388..>11507)
                     /locus_tag="PLQ54_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91683.1"
                     /translation="RGAPPSYNRRPYPCPKPVGELALQLPPDLPGLRIRHELSIEEGM
                     LRTAVSSALGVEVTIDSFIHPELDVLVVRWGVTGWTDETRIGNGKPPVWLSLYRWADP
                     ALREFGDRFFAESRHGTFINSDDGQATPLAPPGIVDLGRHLAIEQTFAPELTYPAGFV
                     YLMVPFCPGFQMEPATQPATREALIHLMPAQDVTSGWVAVAVPSGNGREAAAAQLAPY
                     AALNTESGPAILDQWAQETREAGEAFWARSGLEVADPLVEGIWYETLHARRCTTRAGK
                     TPPGLFLPSTVRDYSHWHGDYHTNYNFQQPYWGDYTANHVEIGDAYFTGMKYLVDVGR
                     ILADRYYGCRGTFIQLTGYPMVMDDDPLGAVPMGRMAYMTGWAANQYWWRYLYTLDLD
                     WLREVGYPVIRDCALFYLDFMTQGEDGLYHIFPSNQGEDGFTGDPRDYTDRGQVMRHM
                     RYCLRSAIAASEALDTDAELRAQWLDRLARAAGDDGNPPVERQGIERHFAEACAPELG
                     EGRPWPGVLRADPATPWPGAMQWVDLWYAGQYPLIAMAQLRASAVEPERAYKGFRRIV
                     ERWRHPNGLVWAMSLMDYGHAGAWTETLGITAPLQEMLLQSYGGVIRIFPVWPRHVAA
                     RFRTFRAEGAFLISASCAGGAVTSVRIESERGGVCRLYSPWETPFRVVTVGSGENVAV
                     EGPEDGIYQFETEAGGAYALEAL"
BASE COUNT         1842 a         3741 c         4032 g         1892 t
ORIGIN      
        1 cggcgcgggt gggcatcccg cgggtggtct cgcatatcgg gttcgtgccc gaggacgacc
       61 ggcgaaccca cgatgccgtc cgcgacgcgg tccgcgaggt gtgcgactac atgggcgagc
      121 gtagcctggt gttcgccctc gagaccgggc aggagaagcc cgaggtgctc cgcgacttca
      181 tcgagcaggt caaccggccg aacctgcggg tgaacttcga catggccaat ctgatcctct
      241 acggctcggg ttggcctctg cccgcactgg acgtgctgat ggattacgtc gacggcaccc
      301 actgcaagga tggcacgtgg ccgaccgagc cgggcaggct gggcaaggaa cagcctctcg
      361 gaaagggcca ggtgaacgtg gaggccgtga tccagcggct gtacgatggc gggtaccgcg
      421 gcccgctgga catcgagcgc gagatcgagg gcgagcagca gcgcctcgac atcatggagg
      481 ccattgagct acttcggggt attcgggccg gtctcggtct ataggggcgc cgggtggttg
      541 gggcaactcg ccagtccatg ggttcggagc gtgcgggggg taggcatggc cgccccccgc
      601 ttgcgttgcc gtggggccta ttggtggtta ttggcttcag tttggcgtcc ggggcctggg
      661 cccaggacga gggggcggtg gcgcggcggg cgcggcggac ggatccacgg tcggcggggt
      721 cgccgttggc cgacgtccac gctctcctga cgaacgcgtg gcggctcgac ctggcgggca
      781 actgcctgtc ggtgatgccc cggcgcccgc gcccgggctt cgtgggcagc gtgggctaca
      841 tccatcctgt ggactggccc gggctgtttg aggtggaggt cacctgtgcg ggtgccccgg
      901 ttcagtggga gccggcatcg catacgtggg gaccggcggg gatgcgtgcc gagtatgcgg
      961 gcggcggcct cgcgctacgc gaggccaaga ccatcacgcg gcacgatgtg gcagcatgcc
     1021 agctgatcgt gacccgaacc gccgcggagt ccgccgggat cgaggtgtgg attcgcccgc
     1081 tgcttcccgg cgcggaggat ctgagggccg agggcgatac cctgtttggg acggtggccg
     1141 tccatctccg gcccagcggc ctgtcgccca tggacttcgt caagcactgg gcgcttcggt
     1201 gccccggagc ggaggcattg gccgagcccg cgggggtccg acggagcgcc acgttgggcc
     1261 cgggggagag cctgacactc acggtggccc ttgcgctccg cgatgagccg ggagcgtgcc
     1321 tgatcccgat cggctcgatg gtgggtctga gcgacccggt ggcggccaac gccgagcggg
     1381 agaacgcctg gtacgccgat cgggtgccgc ccttcgcatg cagcgacccg ctgatcacgc
     1441 gcttgtacta ctaccgatgg tacctggcac ggcggaacac ggtcgacgcc cgggcgggct
     1501 tcgccgggca tccctacgac cgcccctgcg tgttcgagag ccgaagcggc cgggcgtccg
     1561 cccgtctctc gcctcatgcc ctggcgcaac agctcgtgga tctttcatgg atgcgagtcc
     1621 ccgatgtcgc gcaggacctg gcgtgggtcg ttctgcggca gggccaggtg ctcgccgagg
     1681 cgtcgggcgg gcgcacgccg gatccacccg actggacgcc catggccctg tggcgtctca
     1741 tgctcgtcca ccccgatcca gccctgctcg ccgcggccta tcccgaggca tgccggctgg
     1801 tgcgcggctg ggcttcagag agccacgacg tcgaccgcga cgggcgtccc gaggttcgcg
     1861 ggagcttcgc gatgggcctc gagtacctac ccagcttctt ctacttcacg cagccccggt
     1921 gggaccatac gcagggcgag gagttcggac gggccgccgg gctggagcgg cccgacacgc
     1981 agggcgactt ctacctcagc gccatggcat gtgccgcgat ggcggtggcc ctggggcggg
     2041 aagccgaggc gcgggagtgg cagagccttg cggatcgggc caagtccgcg gcgctggagg
     2101 tcatgtggga cgccgggatt gggttcttct gcccggtgga gccgcggacc catgaacggg
     2161 ctccggtcaa ggaagccacg gggtttgtgc ccttcatcgc cgggctggcg gggccggagc
     2221 atgtcgtggc cacggcggcg ctttccgacc ccaaccagtt cggtacgcca ttccccgtgc
     2281 catcggtatc gatggactgc gaggcgttct cgcccagcga tacatggatc gccggaccga
     2341 atgcctcgcc ccagcggccg cacatgtacc gccgcgctca caacggtccg agctggccgc
     2401 agctcacgtc cctggtggcc gatgcggcgg cctgcgcgtg ccggctagct cccggtgacg
     2461 acacaccgcg aatggccttc gccgagctgt tccgcaagta cacccggatg cacttccggg
     2521 gtgatgaccc gccgactccc tgtgtggtcg acgagtacca cccgctgacg ggcgagtggc
     2581 tgggcgactc gatggactgt ttcgcctcgc actacatcga tctcgtcgtc cggcatgtgg
     2641 cgggtctgca gccccgtgcc gacggcgttc tggagatatg gcccatcgac atcggtctgc
     2701 aggcgttctc gctgcaggat gtgccctacc agggccatgt gatcagcatc atgtgggagc
     2761 ggggcggccc ggggctgatc gtgaccatgg atggcaaggc cgtggcccgg cagccgggtc
     2821 tctcgccccc cataacggtg cagatgcggt gaggtgagcg aggccaggac atggggctgt
     2881 acgagcagga tgcgcagcgg gggctgaggg agccgggtat tgggaggttg gcgcgggtcg
     2941 ccatcgtcga gaacggcgat cccatggtgc gactcgactc ggagcgcctg ggccggcgcg
     3001 tgttcatcca ggcgaagctg ccctgggtgc gagtggccgt ggcggacatg ctcacacagg
     3061 cccttgcgtc tcttcctgag ccgctcacgc tccgaatctc gaccgcctac cggaccctgc
     3121 agatgcaacg ccggggctat gagcgctata tgcggcaact ggcgcgggag cacccggagt
     3181 ggccccgggc catcctggtg cgccagtcca accggttctt ccatcccgtg acctcaaagg
     3241 ctccccccgg gcactgcacg ggcggggcgg tggacgtggc cctggtccgt tcccgcacgg
     3301 ggacgctgct ggaccacacg tccaccaccg tcagtgtgcc gaactcgtgg cccacattct
     3361 gccccgtgct gactgacacc gcgcgagcca accgtgcggt gctctatgag gccatgcgtg
     3421 cggcggggtt ctcgaactgc ttcgacgagt ggtggcactg gtcgtacggc gactccggtt
     3481 gggcagctcg cttggggcac cgccatgcgg tgtacggact cgtgaccgag tacccgccgg
     3541 aaatggagga agagatcctg cgcaagcggc cctccggcat ccgccgtcgg gacatccgtc
     3601 ccagtcttga gggcaaccgg tagatcgccg atggcggacg tggcgtgagg gccgtctggc
     3661 tcagcccgcg cgccggagtg ccacggccac gccgaagcgg cggaggacat cgatggcaaa
     3721 cagtgccagc atcccgatgg ccagggccac tggcaacgta tagcggacgg ttcgggccag
     3781 ccactggaac cggggcgggc caccggtcat ctcgagcact tgcgcgtact tggcccgggc
     3841 gaagcgcgtc cagcggtcga tctcgacgtt cttcgcgtcc gccttcttcc acacatcggt
     3901 ggccaggcgg gtggcttcgt cctcggtcac cggcatggcg cacattcgct ccaggacgtc
     3961 cggcggcatg ccggcccgtc ggcacgccgc ccacgccgcc tggagttcgt cgtgggcgtc
     4021 gatcaccagg gcgccaatga ggtcgttgac cagagcccat ctcaatccgc ctcgcttggg
     4081 gtcgtagcgg agagaggaag ggagcgtgaa gggatttacc ttggtgaggg tcttcgggcc
     4141 caccttctcg tagacatcgg gccggacgct catcctgccc agagcgaacc gctcggggcc
     4201 acccggcgtg ccgggctcga gcaggaggag catctgccct tcggtcgaca tcacatagtc
     4261 gatgaaccgt tcagccaccc gccggttggg cgcccctttg aggatgccga tgccgtccgg
     4321 gttcacgacc gtctggccgg tgggcatcac gaactccatg acatcggagc cactctcggc
     4381 gacctgtgcc agagcgtact gatcgatggc catgccgacc gctgcttcgc ccagggcgac
     4441 atccttgggg gtctgtgagg aggagttcgg gaaggcccga atgttggctc cgagccggtg
     4501 gatgacctgg aagcccttgt cccacccgta ggcttggagg atgatctcgt acatcatgtg
     4561 gatgctgccg ctggaccgcg ggtcggccga accgacccat ccggcgagct caggccgtcc
     4621 caggtcctcc caggttctgg gcgtcgggag ccccatctgg cggagcaccc gatggttcac
     4681 caggatgccg aagcctgaca gcgcctggcc gtaccagagc agatccgcat cgtacatggg
     4741 aacgccgcag taggtcggcg gcatggcggc cagcacggcg ggatcgatgc ggtatggcac
     4801 gtacaggccc tctttggcga actcgatgtg cggatcgatc cctccgcccc agaacagatc
     4861 gatgccgatg ctatccttgc cgccggcgaa ctcggaacgg atgaacttga cgtcatcgga
     4921 tgtgccgccc tggtcgagcc actcgaccgt gaccttctcg ccgtagcggg cctcgtacca
     4981 ggcctcgaat gcccggccgt actcgtaccg gattccctcc cagtgcggcg tgataatgat
     5041 gaccttggcc gcatccgccg cggggccacc cgccagggca gccacgagag cgaacggcac
     5101 gagcccgcgt gccatcgggt acctcctgat ccggcgcgca cggcgccgca acatatcgct
     5161 gccatgggtg cggtcattcg cgcccgaacg gacggagcct gcctcgacac catcaggggc
     5221 tcagcagtat tatcgaatct ttgcggcgat tggctccgga ggccgccaca ggcgtcgcgg
     5281 atgacggggc tctgcatgtt gtaccgggtg agggatcggt tgtgaagatc ggcatcatcg
     5341 gccccgaggg ttcggggaag tccacggtat tcgcgtgtct gaccggcctg ggggtgccgg
     5401 cggccacccg gagcccgagc ctcggggtcg tgcgcgtccc cgacaaccgg ctcgaggtcc
     5461 tcagcgccat gtaccagccc aagaagacga cgccagcaga gatcaccttc gtcgatctgg
     5521 ggtcggccgg tcgccacgaa cgcctgtcgg cgattaccgg ctgcctggcc gacgcggatg
     5581 cgctggccat cgtggtggga ggcctgtcgc ctgactcacc cggcgtggcg ctcgagcgct
     5641 tcatgctgga cctgattctg gcggaccttt cggtggtcga aaaccggctc gagcggctcg
     5701 aaaaggactg gcagcgaggc cgcaaagagg ctgcagagga gcgcgacctc atggggcgtc
     5761 tgcaagccgc cctgtccgag ggacgtcgca tcgccgaggt gccgctggac gaggccgatg
     5821 aagcacggtt acgtggattc cagttgacca cacgcaagcc caccgtggtg ctggccaatg
     5881 tgagcgaaca ggacctgggg aacggtgtgg cgcagtccgt cgcggccatt gccgagcccc
     5941 gggccctgcg gaccttcgag ttgtgtgctc cactggaggc cgagatcgcc gagctggccc
     6001 acgacgaacg ggccgccttc ctggccgact acggcctcga gtgcgcggcc accgaccggt
     6061 tcatccgggc ggcctatgag atgctcgacc tgatctcgtt cttcaccgtg gggccggacg
     6121 aggtgcgggc ctggagcgtc cgacgcggga ccctggcccc cagggccgcc gggaggattc
     6181 acagcgacat cgagcggggg ttcatccggg ccgaggtggt cgcgtatgag cacctctcga
     6241 ccgccggatc gatggcagcg tgccgtgccc agggccaggt tcggctcgag ggccgcgagt
     6301 acgaagtccg cgacggcgac gtgatcgagt tccggttcag cgtgtgacgg cccagcaggg
     6361 ggccgcgcca gtgatgtgga agtggccgaa cgtggagaac ggccgggtgt ggacgcgttg
     6421 ccagagagag gtggtcgcgg gtgaagctgg ccatctgcaa cgagatgttc gagaagaggc
     6481 cattgggtga ggtcatcgac ttcgtcgccc aactcggata tgacgggatc gagttggcgc
     6541 ccttcaccct ggccggccat gccgatgacg tgctacccgc cacgcgggca cagattcgaa
     6601 agcatgccag cgatgccggg atcagcgtcg tcggcctgca ctggcttctg gtgacgccgg
     6661 ccggccttca catcgcagcg cgcgaccccc aggtgcggaa gcagacaatc aagtacctca
     6721 agtcgctcgc gaccctctgc gccgacctgg gcggcaagat catggtgttc gggtctccca
     6781 agcagcgaaa cgtgcctgaa ggcgagtcgt acgacgagaa ctggcttctg gccgtagaca
     6841 ccatccgaca ggccgggcag ttctgccagg ggctgggcgt tgtcattgcg atggagccgc
     6901 tgagaagtga cctgacgaac ttccttcaga ccgcggccga gtgtcgccgt ttcgtcgatg
     6961 aggttgccct gccgagtgtg cgtatgacac tcgactgcta cagcatggcg cccatggagg
     7021 agtccattcc ggctgcccta cgggcccagg ccgacgtggt cagccacgtt cacctcaacg
     7081 acgacacggg gcgcgagccc ggcacgggtt cggtggactt cgtggccgtg ggtcaggcgc
     7141 tgcgagacat cggctaccag ggctacgctt cgatcgaggt gttcgagttc aaacccgacg
     7201 ccgagacgat cgccacgcgc gggcttgcgc atattcggca ggcgttcgga ctgtagcggg
     7261 ctggcgcggt gctgaacggc ggggcataat agcgcgttga gcacgcatgc cgagcgcgac
     7321 cgcgcctcgc accaagatca ctggagggtg acactttgca taagaaggac tccatgactc
     7381 gccgggactt cctgggcaag acggccacgg tggccgcggg cgtcagcgcc ttcgcgctcg
     7441 cgtcgccgcg ggccctgggc gccaatgagc gcatccgcgt gggcatgctc ggcccgggtg
     7501 gtcgaggccg ttacctgatg gaccgcgtca accagatcga caatgtcgag ttcgttgccg
     7561 tggccgacat cttcgaggga tggaagaacc tggggctcga gattgcccgc cgcaagaacc
     7621 cgcgcgccaa gggctatgac ctgctcgaga ggatgctcga ggacaaggac gtcgatgcgg
     7681 tggtggcggc cacacccgag catacgcacg tcgaccacat catcaacacc atcaaatccg
     7741 gccgcgacat ctactgcgag aagcccatga accacacatg ggaagagggc atccgggtca
     7801 tcgaggccaa caagaaggcc aagaggatca tccagatcgg cacccagcgc cgcagtgtgg
     7861 acctttacta ccaggctcgt gaactggtcc agtcgggcgc catcggccgg atcaccgcgg
     7921 tccgcggccg ctggcaccgc aacagcaagg acaacgaccc gcagtggcgt tacgcgattc
     7981 cgcccgatgc cagcgagaag aacatcaact ggcccgtgtt cctgggcaag gcgcccaagg
     8041 tgcccttcag cctcaatcgc tacttccagt ggcgctgcta ttgggactat tccaacggcc
     8101 cggcggggga tctgatggtc catcagttgg acgcgatcaa cctcgtgatg ggcgggcaga
     8161 tgccgaagtc cgccatgggc ctgggcgaca tctaccgttt ccatgagctc ggccggacca
     8221 cgccggacac gtggtcatcc atcctcgagt atcctggtgg cagcccctgg gcaccggatg
     8281 gctacctggt ccagtacagt tgcgtcttca gcaaccagca ggaccagtat ggcgagatct
     8341 tttacggaac cgatggcacc atcgacatga atgaccgcgt gctccgcctg atcccggagc
     8401 ccgagggtgt cgccatgaag aaggtcgagg ccaaggagtt ccagacccag gtccccgacc
     8461 acggtaaggc gcacctcgag aacttcttcg actgctgccg cacccggaag cagccgaact
     8521 gcgatgagta ccagggccac tacgctgcct cggcggccca catggcggtg ctctgccact
     8581 tctccggtga gcgcaccctg tgggatgaca agaagcagcg ggtcgagtag gcggccccgg
     8641 ccaaccgagt gctggacttc gggggcagcc gcgcgtgcgc cggctgcccc ctgtcattcg
     8701 ggagtggggg ggtgagtgcc atcgagatgg agtcgtgggg cggcatcgcc aaggccctgg
     8761 tctcgctcgt cgccgtggtg aaccccctgg gctcaatccc cgtgttcctc agcgtcaccg
     8821 ccgagcggac tcccgacgag cgccggcgca tggcgcgcat caccgccatc tccgtcggca
     8881 tcacgctcat cgtgtcggcc ctggtcggcg aacaggtgct gcgcatgctc ggcatcacgc
     8941 tgccggcctt ccgtgtggca gccggtgtcc tcgtgttgct catgtctctc cacatggtgc
     9001 aggcgagccc cagccggacc cagcacactc ccgaagaggc ggccgaggct gaggaccggg
     9061 agggcgcggc catcgtccca ctggccatac cgctgctttc gggtcccggc tcgatgagca
     9121 cggtggtgct ctactcgcag ggctgccggc ggtggcagga gatggcggtt ctggtcgcgc
     9181 tatgtgccgc catcggcgtt ctggtgtggt tcgtgctgtc ggcggcagcg cgtctgcgcg
     9241 cgcggttggg acaaacgggc atccgggtcc tcacgcgcat catgggcgtg ttccttgccg
     9301 ccatcgccgt cgagttcatc acatcgggct tgggcgattt gctgccggga ctggtgaccg
     9361 tcaagggacc gtaggcacgg gtcgtcccta cagggcctcg agcgcgtatg cccccccggc
     9421 ttcggtctcg aactggtaga tgccgtcctc ggggccttcg acggccacat tctccccgct
     9481 accgacggtc accacacgaa acggtgtctc ccacggggaa tagagccggc acacgccgcc
     9541 tcgctcgctc tcgatacgca ccgaggtgac ggcgccaccg gcgcagctcg cgctgatcag
     9601 gaaggctccc tcggcgcgaa aggtccggaa gcgggccgcc acgtggcgcg gccagacagg
     9661 gaagatgcgg atcacgcccc cgtagctctg cagcagcatc tcctgcagcg gcgcggtgat
     9721 gcccagcgtc tcggtccacg ccccggcgtg gccgtagtcc atcagcgaca tggcccagac
     9781 cagaccgttg ggatgccgcc agcgctcgac gatacgccgg aagcccttgt aggcgcgctc
     9841 gggctcgacg gcgctggccc gcagctgggc catggcgatg agcgggtact ggccggcgta
     9901 ccacagatcc acccactgca tggcccctgg ccatggggtg gccggatcgg cccggagcac
     9961 accaggccaa gggcggccct cgcccagctc gggagcacag gcctcggcga agtgcctctc
    10021 gatgccctgc cgctcaacgg gcggattccc gtcatcgccg gcggcccgtg cgagccggtc
    10081 gagccactgg gcgcgcagct ccgcatcggt gtccagggcc tcgctggcgg cgatggccga
    10141 tcgcaggcag tagcgcatgt gtcgcatgac ctgtccgcgg tcggtgtagt cccggggatc
    10201 acccgtgaag ccgtcttcgc cctgattcga ggggaagatg tggtaaagac cgtcctcgcc
    10261 ctgggtcatg aagtccaggt agaacagcgc gcagtctcgg atcaccgggt agccgacctc
    10321 gcgcagccaa tcgagatcga gcgtgtacag gtagcgccac cagtactgat tggcggccca
    10381 tcccgtcata taggccatgc ggcccatcgg cacggctccg agcgggtcgt cgtccatcac
    10441 catggggtag ccggtgagct ggatgaaggt gccgcggcaa ccatagtagc ggtcggcgag
    10501 gatgcgtccg acatcgacga ggtacttcat gcccgtgaag taggcatcgc cgatctccac
    10561 gtgattcgcg gtgtagtcgc cccagtaggg ttgctggaag ttgtagttgg tgtggtagtc
    10621 gccgtgccag tgggaataat cgcgtaccgt gctgggcagg aacagcccgg gcggcgtctt
    10681 tccggcgcgt gtggtgcacc ggcgggcgtg tagcgtctcg taccagatgc cctcgacgag
    10741 cggatcggcc acctcgaggc cggagcgcgc ccagaacgcc tcgccagcct cgcgcgtctc
    10801 ttgggcccac tggtcgagga tggcggggcc cgattcggtg ttcagagccg cgtagggcgc
    10861 aagctgggcg gcagcggctt cacggccgtt gcccgacggc acggccacgg ccacccagcc
    10921 cgacgtgaca tcctgagccg gcatcaggtg gatgagagcc tcgcgggtgg cgggctgggt
    10981 cgctggttcc atctggaacc cggggcagaa gggcaccatg aggtagacga atccggccgg
    11041 gtaggtcaac tccggcgcga acgtctgctc gatggccaga tgccggccca gatccacgat
    11101 gcccgggggg gccagcgggg tggcctggcc gtcgtcgctg ttgatgaacg tgccatggcg
    11161 tgactcggcg aagaagcgat cgccgaactc gcgcagagcg gggtcggccc atcggtagag
    11221 cgagagccac acgggtggct tgccattgcc gatgcgtgtc tcatcggtcc agccggtcac
    11281 cccccagcgc acgaccagca catcgagctc ggggtggatg aaggagtcga tggtcacctc
    11341 gacgcccaac gcgctcgaaa ccgcggtccg cagcatgccc tcctcgatgc tcagctcgtg
    11401 gcggatacgc agacccggga ggtccggagg gagctggagg gccagctcac ccacgggttt
    11461 ggggcatggg tagggccgtc ggttgtagct cggaggagcg cccctgc
//
LOCUS       DAOSPL010000003        11264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_5747_length_11264_cov_4.815505, whole genome shotgun sequence.
ACCESSION   DAOSPL010000003 DAOSPL010000000
VERSION     DAOSPL010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 11264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11264
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5747_length_11264_cov_4.815505"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1989)
                     /locus_tag="PLQ54_00085"
     CDS             complement(<1..1989)
                     /locus_tag="PLQ54_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91684.1"
                     /translation="MAFACTVVTLAYTQEARLNFHQAAAFLIASDGMSGGEEVCAAHV
                     CGIGFDFVEELAEIYEEYTISNIYLRATLPGMPPTIYKRLVGHRKTYSDEWLGQELTL
                     DEEALHTTCSMSFPVRLWKMRDASEKPESLANPCHPLHLAMHYKWKTDPDAQVTYDCG
                     FGSASSAISYEGADLPAFSPTLTLKRTPYFPVSGFAVSITGAKLLDRSISFDGYQASG
                     NGCAAAGDGDGMWLEVVNWAQVPLSPPTSFTAGVSPPVIVDFDLHGQDWSASKALRFR
                     IPRNVPDGTGRTFDVVAAPFHEEREYWSKTWGGPWLRPFSLALSLDPDWMTEEDENVD
                     PSDLACTIEESDLSVEADYEWPHFRVRRTPGLSVQVPPDAPSIPSPWVPQSGLTVEQV
                     GKTTRVTVHPGTTAGTLVRTLREDYFRAYLTPEGLGTYGLPGAYRYLKHQPGSDIWNW
                     ENYQFIRLEYESERPAVLALTVDYSAVTVHDNHLTGQIRVDEFSVDRERRQASYLLPI
                     RATEPPEVAELVIDLGVPNDLKLQHVDALTLSGFTNPQALAEPWLFRLHEMEIRRYNP
                     ITDYGVGRTACTVTFNRPAEGEGELGLPLSYTAFTAVAEGGRCCRPPDQIRTCCGEEG
                     LDFVEQLTGSGSGLCLDFMRPLSQWYTELARQEGWEVDH"
     gene            complement(2002..2217)
                     /locus_tag="PLQ54_00090"
     CDS             complement(2002..2217)
                     /locus_tag="PLQ54_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91685.1"
                     /translation="MFDALREAFRQLTRFEAGTRTLLREVDDDPLIFNATHSEPEGVI
                     AEGPELHFIANEATLGLLDDEAEWELG"
     gene            2573..3301
                     /locus_tag="PLQ54_00095"
     CDS             2573..3301
                     /locus_tag="PLQ54_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91686.1"
                     /translation="MMCFQRQAREAVAVLAMSLALVGAVWIACAQDEQAAEAGDAPPS
                     LEEVRQALAAETTDPVADADESERFVVSFRFAAGWWTGDGPAPTFEELVGREGQVPPS
                     IPKRDFFDEEAMARIANRTGRLDVRVSPIYSLVCSAEVPGRAELKAPNRYFSWEATAT
                     RTLGDNVRLDVIWKTLRGPRGTDEFFGPGGVFYRDRDGNARRGLKGTREFAIGEAYRC
                     LCQSPGPEQANLVLCYLLVEEAWP"
     assembly_gap    3307..3406
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3407..5025)
                     /locus_tag="PLQ54_00100"
     CDS             complement(<3407..5025)
                     /locus_tag="PLQ54_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91687.1"
                     /translation="MSLPLRAQTKVMVFPPNYTGTVEHQGVGYEWMVRPEEASTPTNT
                     ARGIFHTYWYMGKGTPKLYYVPEPVPFVQVLHTHQQIKTFTDPAGRPVLLYDAPVRLS
                     GQPVTDFNTALGDHGFLFRGAATRWGLVDIPCISSGPFGSGITTLGATVQAVENFTLR
                     VLPVLEYQRGSWSLRVNFNQKHFFWWAQQPGSKNGESYFSFHRYTDYDTDQSKQIIPA
                     GHLPVRAAGNWLQFHFIAFGKDVLLVEILDADGKALRRFMAYDPELNLYGKDVEIELA
                     VNDGTLALGVTEHRYSATGVPPSSVPDEDELNDPDAIDDDLALTYPLHAGVEVLNPNT
                     PTGTAIGVAPEGTSFRVYTQRRTGSDVYRPVVALRSTQTRTPVLVGAQLVWPSTLLPG
                     TGTGTALTQLRSAPVAYYSKDYRHNRLRLDYAVGSQPHLKGNECIEVSLQTVRQDGTV
                     EAEQHLFGGFVSLYGTRQAGRDRETVAHLEARDWTLRLSKKDMGDTPCFAGWPLLDAV
                     RFIFHRCGISDNLVHWEQSSEGWTVPQRALF"
     gene            complement(5159..5398)
                     /locus_tag="PLQ54_00105"
     CDS             complement(5159..5398)
                     /locus_tag="PLQ54_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91688.1"
                     /translation="MNPDQQKPTPEEPQDDYPTRTRAKEDLQRAGLPQWEKPVIGPDG
                     LWTKSFAELAADLVGGEGAPPDLSYNPDHMRDFGK"
     gene            complement(5388..5864)
                     /locus_tag="PLQ54_00110"
     CDS             complement(5388..5864)
                     /locus_tag="PLQ54_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015127877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOS91689.1"
                     /translation="MYLLDTNSLSQILKGDPALDAWLDAKDDAALATCVVVRGELVYM
                     AENSDRRAENLQDTEALLAGLTIHDIDEATADAYGQIKAAIFRCFGPKEKAKRRQTEW
                     KTLGVDENDVWIAAVAKQRGLTVVSVDSDFQRIHQATGLPVENWLSPPTGQPTDES"
     gene            complement(6128..6880)
                     /locus_tag="PLQ54_00115"
     CDS             complement(6128..6880)
                     /locus_tag="PLQ54_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91690.1"
                     /translation="MFEPARYFSSNLTVGFPRRTDVETCADAIAAKLEERYKPYPMQP
                     LPEWLEQDVPRLAFVTPKQVIGSHRIYVTQAGFRLEVFHKPSHQLEPSVVEESLIAEV
                     ATLFSLLETVQAESPTYCGLMTQVRVPAESETEDRSIGRYLAKRFLKEPEISGLYELT
                     VRFCETIDERFFSNTTYEVYRAWDSAKMDPAKPRLSGPGAMTRGVLISGDFNDRHAFN
                     ENEEYTSNQQTAEEIVHRGMDAVARAITTIRG"
     gene            complement(7109..7252)
                     /locus_tag="PLQ54_00120"
     CDS             complement(7109..7252)
                     /locus_tag="PLQ54_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91691.1"
                     /translation="MTHSQPVAPEGIPWLREASSREAEASPTDAMDLTPLLAEALRQL
                     GRE"
     gene            complement(7724..8179)
                     /locus_tag="PLQ54_00125"
     CDS             complement(7724..8179)
                     /locus_tag="PLQ54_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91692.1"
                     /translation="MALHRRLAPRRFTETVALASDPEVRFYLRFSAQEADELANFVAP
                     PQWGHLCRTAAGFVQDWEGVCAEDGTPVPWSLEEFYGRLTAAERLEFAQATVALVYGE
                     AAGWGSEADPFATNAGGCHCSSPESPGNPNGVLSPKPCSSIPSSASIPV"
     gene            complement(8212..9045)
                     /locus_tag="PLQ54_00130"
     CDS             complement(8212..9045)
                     /locus_tag="PLQ54_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91693.1"
                     /translation="MAHKTARVLSCRWGIASPDTPVGRVFGGTCVVPQNLTNSRAIGG
                     QEVNLYGLIEPRIQNLAFEVQSGALLSQAVRGAGSLPALKFLCSTDAGSFLFEDAYIH
                     EMHLRFAGAGTLTASLSIMALKVSPSTAQAPASLNLPVYQWHDGQMTLGGAGRSMAEG
                     VVSFSNNLEYLLNWDGPIEASRARFPSEIKTNTFESLACRFTLREPHGTLDPLANAPG
                     TTGAVIRAERGSHHVTFTLSGLSLAEDELLPIPGGADEVIEYHLPFYGLHGSLVVTAS
                     E"
     gene            complement(9151..9651)
                     /locus_tag="PLQ54_00135"
     CDS             complement(9151..9651)
                     /locus_tag="PLQ54_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91694.1"
                     /translation="MSPHLWTDALADYLVGLIQDHLGEALNLSRVAKIRPDELAPIAF
                     SDPLDDALPAVYVILDDPRILPARIIGPVVDVEERFRLAFLKRGPIDQADTAALNADI
                     QSLAALMRDHPWVEPGVLPAPTQQLLPLAVDGVEYDPPELRPLRKLDAALAGCALPVR
                     IVTTTA"
     gene            complement(9806..10111)
                     /locus_tag="PLQ54_00140"
     CDS             complement(9806..10111)
                     /locus_tag="PLQ54_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91695.1"
                     /translation="MSDFPSYRVTVTRPVQTPGGTEVEVHYRTVAREVACELYSEETQ
                     GVKSLPGTRPLRYAQAIISPRADVRFGDRLVEPTGQCWVVDHLVRSDLVCRCTLREG"
     gene            complement(10281..10772)
                     /locus_tag="PLQ54_00145"
     CDS             complement(10281..10772)
                     /locus_tag="PLQ54_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91696.1"
                     /translation="MPTPLDIEVGGSTANSYAGRNEADQYFADDPNRAQAWEGFPPAQ
                     RDRALLLATRWMERLALRGEPLTNDQALHFPTAETLDRQGEAYIPIGLKYAQIEWAFH
                     ILAHAPETVLDRASLRAQGVRSFSRLGLSEQLTPGPGTPPLPPEVRAYLQPWLIAGRR
                     ISR"
     gene            complement(10874..>11264)
                     /locus_tag="PLQ54_00150"
     CDS             complement(10874..>11264)
                     /locus_tag="PLQ54_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91697.1"
                     /translation="LLAAHNAPEPYFAVISPYVEEDLIQLLSGNTNQFGDSVTEEIVR
                     TGYVGNPYGVITYRSGNLIQDVNGDVRCAMFSREAIGMVIKWDPRIEIQRDASLRGYE
                     VIMTTAYGIDTIDANFGCSMLLDNADA"
BASE COUNT         1846 a         3369 c         3887 g         2062 t
ORIGIN      
        1 atggtcgacc tcccaccctt cttgccgcgc gagttcggtg taccactgcg agagcgggcg
       61 catgaagtcg aggcatagcc cgctgcctga tcccgtgagc tgctcgacga aatcgagccc
      121 ctcttcgccg cagcacgtgc ggatctgatc cggcgggcgg cagcatcggc caccttcggc
      181 caccgccgtg aaggctgtgt agctcagggg caggccgagc tctccctcgc cctcggccgg
      241 ccgattgaat gtgaccgtgc aggccgtgcg gccgactccg tagtcggtga tggggttgta
      301 gcggcggatt tccatctcgt ggagccggaa gagccacggc tcggccaatg cctgtgggtt
      361 cgtgaatccg ctgagggtca gggcatccac gtgttggagc ttcaggtcat tgggcacgcc
      421 gaggtcgatg acgagctcgg cgacctccgg cggctcggtg gcccggatgg gcagcagata
      481 gcttgcttgc cgccgttccc ggtcgacgga gaactcatcc acccggatct ggccggtgag
      541 gtgattgtcg tgcaccgtca cggccgagta gtccaccgtc agcgccagca cggcgggccg
      601 ttcactctcg tactccaggc ggatgaactg atagttctcc cagttccaga tgtcgctgcc
      661 cggctgatgc ttcaggtatc ggtaagcccc ggggaggccg tatgttccca ggccctcggg
      721 cgtgaggtag gcgcggaagt agtcttcccg cagggtccgg accagtgtcc ccgccgtggt
      781 gccgggatgg acggtgactc gagtggtctt tcccacctgc tcgacggtta gcccggactg
      841 gggtacccag ggcgagggga tcgagggtgc atcgggcggg acttgcaccg acaggccggg
      901 cgtccggcga accctgaagt gtggccattc atagtcggcc tcaacgctga ggtcgctttc
      961 ctcgatcgtg cacgccaggt cggatgggtc gacgttctcg tcttcttcgg tcatccagtc
     1021 cggatcgagc gagagcgcca gactgaaggg ccgcagccag gggccgcccc atgttttgga
     1081 ccagtactcg cgctcctcat ggaagggcgc ggcgacgaca tcgaaggtcc ggccggtgcc
     1141 atcgggcaca ttgcgaggga tgcggaaccg cagggccttg ctggcgctcc agtcctggcc
     1201 gtggaggtcg aaatcgacga tcacgggcgg agagacaccg gcggtgaagg atgtgggcgg
     1261 cgataagggg acctgcgccc agttcacgac ctcgagccac atgccatcgc catcgcccgc
     1321 tgcggcacag ccgttcccgc tggcttgata gccgtcgaag ctgatgctgc ggtccagcag
     1381 cttggccccg gtgatgctga ccgcgaagcc ggagaccggg aagtagggtg tacgcttgag
     1441 ggtcagtgtg gggctgaagg ccggcagatc ggcgccctca tagctgatgg ctgaggaggc
     1501 cgaaccgaag ccgcaatcgt aggtgacctg ggcatccgga tcggtcttcc acttgtagtg
     1561 catggctaag tgcagcgggt gacacgggtt cgccagactc tcgggcttct cgctggcgtc
     1621 gcgcatcttc cacagccgga cggggaagct catggagcag gtggtgtgca gggcttcctc
     1681 gtcgagggta agttcttggc ccaaccactc gtcggaatag gtcttgcggt gtcccaccag
     1741 gcgcttgtag atcgtgggcg gcatgccggg cagcgtggct cggaggtaga tgttgctgat
     1801 ggtgtactcc tcgtagatct cggccagctc ctcgacgaaa tcgaacccta tgccgcacac
     1861 gtgagccgcg cagacctctt cgccccccga catgccgtcg gaggcgatga ggaaagcggc
     1921 cgcctggtgg aagttcagcc gggcctcttg ggtgtaggcg agcgtgacaa cggtgcatgc
     1981 gaaggccatt ggcgtgcgtt cctaacccag ttcccattcg gcctcgtcat cgagcaggcc
     2041 gagggtggct tcgttggcga tgaagtgcag ctcgggccct tcggcgatga cgccttcggg
     2101 ctcggagtgg gtggcattga agatcagcgg gtcgtcgtcg acctcgcgca gcagggtccg
     2161 agtgccggct tcgaaccggg tgagctgccg gaaggcctcg cggagtgcat cgaacatggg
     2221 ggctccttcc acctctcccc gcgggagagg tcggctccga gcgggagcga ggagacgggt
     2281 gaggggcggt gccggaaggt cttcggaggg gccgaaatgg gggaaccatc cgcccttcga
     2341 caggctcagg gctggtggga ccctatccgg cccgcttgca tacgggcacg gtgccagact
     2401 gcggcagtgc tttggacggg gtgtcatccc gggcgggcta cgggatgtgt tagatacgat
     2461 gctcgattcg ccatttttcc tcaatccgca tggcccgtgc aggtctcgtg cccccccccc
     2521 caagaagcta atctgtacgg tacctgctca gcgcagatgg gaggtgaaat ggatgatgtg
     2581 tttccaaagg caggcacgag aagctgtggc tgtgttggcg atgagccttg ccctggttgg
     2641 cgctgtgtgg attgcctgtg ctcaggacga gcaagccgcg gaggccgggg acgccccccc
     2701 ctctctggaa gaggtacgtc aggccctagc tgcggagacg accgaccctg tggctgatgc
     2761 tgacgagtca gagcgattcg tggtgagctt ccgcttcgcc gcaggatggt ggactggcga
     2821 cggacccgct cctaccttcg aggaactggt cggacgggag gggcaggttc cgccttccat
     2881 cccgaagcgc gatttcttcg atgaggaagc catggcccgc atcgccaatc ggacgggcag
     2941 gctcgatgtg cgcgtgagcc ccatctactc actcgtgtgt tcggccgagg tacccggtcg
     3001 ggcagagctc aaggcgccca accgctactt ctcttgggaa gccacagcca cacgcacact
     3061 gggcgataac gtgaggctgg atgtgatctg gaagaccctc cgaggcccga ggggcacgga
     3121 tgagttcttc ggccccgggg gggtgttcta cagagaccgc gatgggaatg ccaggcgggg
     3181 tctgaaaggc acgcgggagt tcgcgatcgg tgaagcctat agatgtctct gtcagagtcc
     3241 cggacctgag caggccaatc tcgtcctctg ctatctgctt gtcgaagagg catggccgta
     3301 aggatcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttga acagcgcccg
     3421 ctgaggtacc gtccaaccct cgctcgattg ctcccagtgg accaggttgt cggagatccc
     3481 acagcggtgg aagatgaacc ggaccgcatc cagcagcggc cagccggcga agcagggcgt
     3541 gtcgcccatg tccttcttcg agagcctcag cgtccagtcg cgcgcctcga ggtgggccac
     3601 cgtctcccga tcgcgccccg cctggcgggt gccgtagagg ctgacgaatc cgccgaagag
     3661 gtgctgttcg gcttcgaccg tgccatcctg gcgcacggtc tggagactca cctcgatgca
     3721 ctcgttgccc ttcagatgcg gctggctgcc gacggcgtaa tcgagccgca ggcggttgtg
     3781 ccggtagtcc ttcgagtaat aggccaccgg ggccgagcgg agctgggtca gcgccgttcc
     3841 cgttccggtg ccaggcagca gggtcgaagg ccacacgagc tgcgcgccca cgagcaccgg
     3901 tgtgcgcgtc tgcgtgctcc gcagggccac cacggggcgg tagacatccg accccgttcg
     3961 gcgctgcgta tagacccgga agctggtgcc ttcgggcgcc accccgatgg cggtgcccgt
     4021 gggcgtgttc gggttgagca cctcgacccc ggcgtggagg ggatacgtga gcgccagatc
     4081 gtcgtcgatg gcgtccgggt cgttgagctc gtcttcgtcg ggcacgctgg agggcggcac
     4141 ccccgtggcc gaataacggt gttcggtcac cccaagggcc agagtgccat cgttgaccgc
     4201 cagctcgatc tcgacatcct tgccatagag gttcagctcg ggatcgtagg ccatgaagcg
     4261 ccggagcgcc ttgccgtcgg cgtcgaggat ctcgaccaac agcacgtctt tgccgaaggc
     4321 gatgaagtgg aactggagcc agttgcccgc cgcgcgcacg ggcaggtggc cggcggggat
     4381 gatctgcttg ctttggtcgg tgtcgtagtc ggtatagcgg tggaagctaa agtagctctc
     4441 gccgttcttc gagcccggct gctgcgccca ccagaagaag tgcttctggt tgaagttgac
     4501 ccgcagcgac cacgagcctc gctggtactc gagcaccggc agcacgcgga gcgtgaagtt
     4561 ctccacggcc tgcacggtgg ccccgagggt ggtgatcccc gagccgaagg ggcccgagga
     4621 gatgcagggg atatcgacca gcccccagcg cgtggcagcg ccgcggaaga ggaagccgtg
     4681 atcgcccagg gcggtgttga agtcggttac cggctggccg gagagccgca ccggagcatc
     4741 gtagagcaag accggccgcc ccgcggggtc ggtgaaggtc ttgatctgct ggtgggtgtg
     4801 gagcacctgg acgaaaggca cgggctcggg cacgtagtag agcttcggcg tgcccttccc
     4861 catgtaccag taggtatgga agatgccccg ggccgtgttg gtgggcgtgc tggcctcttc
     4921 cggccgaacc atccattcat accccacgcc ctgatgttcc accgtgccgg tgtagttcgg
     4981 ggggaagacc atgaccttcg tctgcgcgcg aagaggcagg ctcatagcgg ggaagcctcc
     5041 aaggggatgg ggggcagtct gagcgggtgg cgttcaagcg ctgcggggcc tagcgtcgat
     5101 cgtcggggat gatcaggggg atgggctggt cgcctcgggg ggggtctgga tagctgtcct
     5161 atttgccgaa gtcccgcatg tggtcggggt tgtaggagag gtccggcgga gctccttctc
     5221 caccgactag gtctgcagcc agctccgcga aggacttggt ccagagcccg tctggaccga
     5281 tgactggctt ctcccattga ggcagcccag ctcgctgaag atcctctttt gcgcgggtcc
     5341 tcgtggggta atcgtcttgg ggctcttcgg gcgttggctt ctgctggtca ggattcatct
     5401 gtgggttgtc ctgttggcgg tgagagccag ttctcaaccg gaagaccagt ggcctggtgg
     5461 atccgctgaa agtcagagtc aaccgagaca acggtgaggc cacgttgctt tgcgactgcc
     5521 gcgatccata catcgttctc gtcgactccg agggtcttcc attcggtctg tcgccgtttt
     5581 gccttctctt tgggtccgaa acaccggaag atcgctgcct tgatctggcc gtaggcatcg
     5641 gctgttgctt cgtcgatgtc gtggatagtc aggccagcaa ggagggcctc tgtatcttgg
     5701 aggttctcgg ctctccggtc ggagttctcg gccatgtaga ccagctcgcc gcgtacgact
     5761 acacatgtgg ccagagcggc gtcgtccttg gcatccaacc atgcgtcgag cgcgggatcc
     5821 cctttgagga tctggctcag gctgttcgtg tcgaggaggt acattcagaa ccgagctttc
     5881 cctcgagtgc gatatacgag gtccagacac tcctcaaggt catcgccatt ccaggttccc
     5941 atatacttcc gcagacttct tgccgtggag aaaccgggct ctggcacggg ttgcagaagc
     6001 atcagatggg gggtgttaag cgagaacgtt ccatgggaag atggcaaagt gccttccacg
     6061 aggcgaagcc ccgtgactac gtccgtctgc gcgctgtcca ctcttagcaa agcctcggcc
     6121 atctgggtca cccccttatc gtcgtgatcg cccgggctac tgcgtccatc ccgcggtgaa
     6181 cgatctcctc ggcggtctgc tgattcgaag tgtactcttc gttctcgttg aaggcatgcc
     6241 ggtcattgaa gtcgccgctg atgagtactc cgcgcgtcat ggctccaggg ccactgagcc
     6301 gcggcttggc tgggtccatc ttggccgagt cccacgcgcg atagacctca taagtggtgt
     6361 tgctgaagaa gcgctcgtca atggtctcgc agaagcgaac ggtcagctcg tagagcccac
     6421 tgatctcggg ctccttcagg aaacgcttcg ccagatagcg gccgatagac cggtcctccg
     6481 tttcgctctc tgcaggtacc cgaacctgag tcatcaagcc gcagtaggtt gggctctccg
     6541 cctggacagt ctccagcagg ctgaacaggg tggcgacctc cgcgatcagg ctctcctcga
     6601 caacagaagg ctccagttgg tggctgggct tgtggaagac ctccaggcgg aagccagctt
     6661 gcgtgacgta gatccgatgg gagccgatca cctgtttcgg agtgacaaag gctagccgag
     6721 gcacatcctg ctctagccac tccggtagtg gctgcattgg gtagggctta tagcgctcct
     6781 cgagcttggc cgcgatggca tcggcgcagg tctcgacgtc tgtccgccga ggaaaaccca
     6841 ccgtcaggtt gctggagaag taccgtgcgg gctcgaacat gcctggcctc cctccgcgga
     6901 cctcgggatt ggcggtctat ccgcagtgta tcccaggagc ctctaggggt gtgctcctca
     6961 gtgcgctcct aacgggccgg atggcgccct tcccgctgcg cggccccttc cattcggccc
     7021 ctccttcggt tcctcggagg gtattcggag gggccgaata gggggggacc agccgaaggc
     7081 tggtgggacc ctatccggcc cgagtgtctc actcccgccc gagttgccgg agcgcctcgg
     7141 ccagcagtgg cgtcaggtcc atcgcgtccg taggcgaggc ttcagcctcg cgcgagctcg
     7201 cctcgcgcag ccacgggatg ccttcaggcg ccacgggttg gctatgtgtc atcaccacag
     7261 ccacgggccc ccacggttgc cggcgagcag ccagggcagg ctccacctcg gttccgctcg
     7321 gccgcgcgtg tatcatcgcg gcgcggattc gctcgatcga gaccggtcgt cgaaggcagg
     7381 cgagtgcggg cagtccgcct ggcattgcag aagttgcccg ggctgccaag gccggcgcaa
     7441 ggaggctcgg taccttccgc acgctcggca gtggtaaagc tgcagggctc tggcggacca
     7501 aagaggtcag cgctctcccc acccacgcgt tcgctcgaac cggtgccggg acgctcgcgc
     7561 ttcggagcct tcgcagtgcc accggcggcg cgagagtcgg cgccagggcg gggagggcca
     7621 gtaccggcgt tcgcttatcc cacagggtga gcatggcgcg cctccataca gcggagcagg
     7681 ccccgcaaca gggctcgggg catggtgtag atgtcggccc aactcagacc gggatactcg
     7741 cggaggaggg tatcgaagag cagggcttcg ggctgaggac cccattcggg ttgccaggcg
     7801 actccggcga agaacagtga cagccgccgg cgttcgtcgc gaaagggtcg gcctcactcc
     7861 cccaccctgc cgcttcgcca tacaccaggg cgacggtcgc ctgcgcgaac tcgagccgct
     7921 cggccgcggt cagccggccg tagaactcct ccagcgacca cggcaccggc gtgccatctt
     7981 cggcgcacac gccttcccag tcttgcacga acccggcggc ggtccggcag agatggcccc
     8041 actggggcgg ggcgacgaag ttggccagct cgtcggcttc ttgggcggag aaacgcaggt
     8101 agaaccgcac ctcggggtcg ctcgcgaggg ccaccgtttc ggtgaagcgt ctcggggcca
     8161 atcggcgatg gagtgccatg gaatcgctcc tgttcggggg gaaaaggcgt cctactccga
     8221 ggcggtcacg acgaggctgc cgtgcaggcc atagaagggc aggtggtact cgatcacctc
     8281 atcggcgccg ccggggatgg gcagcagttc gtcctcggcc aggctgaggc ccgagagggt
     8341 gaaggtcacg tggtggctac cgcgttcggc cctgatgaca gctcccgtgg tgccgggcgc
     8401 gttggccagg ggatcgaggg tgccatgggg ctcgcgcagc gtgaatcggc aggccaggct
     8461 ctcgaaggtg ttggtcttga tctccgacgg gaatcgggcc cgggatgcct cgatcgggcc
     8521 atcccagttg agcaggtact ccaggttgtt tgagaaggac accacgcctt cggccatgga
     8581 cctgcctgcc ccgccgaggg tcatctgccc atcgtgccac tggtagacgg gcaggttcag
     8641 cgaagctggc gcctgggccg tggacgggct gaccttcagg gccatgatgg ataggctggc
     8701 ggtcagcgtt cccgcccccg cgaagcgcag gtgcatttcg tggatgtagg catcctcgaa
     8761 gaggaagctg ccggcatcgg tggagcacag gaacttcagg gcaggtaggc ttccggcgcc
     8821 tcggacggct tgggacagaa gcgcgccgct ctggacctcg aaggccaggt tctggatacg
     8881 gggctcgatg aggccataga ggttgacctc ttggccgccg atggcgcggc tgttggtgag
     8941 gttctggggc accacacagg ttccgccgaa cacacgcccg acgggcgtgt cggggctggc
     9001 gattccccag cggcaagaga gaacacgggc ggtcttgtga gccatggttg ctcctttggg
     9061 ggatggccag aggcgggccg gatggcgccc ttcccgcttt gcgggcccct tccattcggc
     9121 ccctcctctg cctttcggag ggatcgccgc ttacgccgtc gtcgttacga tgcggaccgg
     9181 cagagcgcag ccggcgaggg cggcgtcgag cttcctcagt ggccgcagtt cgggtgggtc
     9241 atactcgacg ccatccaccg cgaggggcag gagctgctgg gtcggggcag gcagtactcc
     9301 cggttcgacc cacgggtgat cccgcatcag ggcagccagg gactggatgt cggcattcag
     9361 cgcggcggtg tccgcctggt cgatggggcc gcgtttgagg aaggccagcc ggaagcgttc
     9421 ctccacatcg accacggggc cgatgatccg cgcggggagt atccgcggat catccaggat
     9481 cacgtacacg gccggcagag cgtcgtccag tgggtcggag aaggcgatgg gggcgagttc
     9541 atcggggcgg atcttggcca cccgcgatag gttcaaggct tcgccgagat gatcttggat
     9601 caggccgacg aggtagtcgg cgagcgcgtc ggtccagaga tggggggaca cggggagctc
     9661 cttctggggc gaaacgcggt cgagcgatgc cagggagggg ccaggagacc gggggcatgc
     9721 cacggggccg gggaggtggc cccgtctaga gacttcgatg ctagctggtg gtgtgacgag
     9781 ttgtcatcag gactccgagg gctgtctacc cttcccgcag ggtgcatcgg cacacgagat
     9841 cgctgcggac gaggtgatcg acgacccagc actgacccgt cggttcgacc agacgatcgc
     9901 cgaagcggac atcggctcga ggcgagatga tggcctgggc gtagcgcagg ggccgggttc
     9961 ctggtaggga cttcacgccc tgggtctcct ccgagtacag ctcgcaggcg acttcccgcg
    10021 ccacggtgcg atagtgaacc tcgacttcag tgcctcctgg ggtctgtacg ggtcgagtga
    10081 ccgtcacacg atacgaagga aaatcggaca tagccatcac acccaactcc ttatccaccc
    10141 cacggctatg caggatcttc actcaggctg ttaccgaaca gaagatttat ttccattgta
    10201 tttacagcaa tctgctgtcc gggtcaaccg cctcgtgcag actcctgtac ccgtagaacg
    10261 aacggcctgc ctattgggat ctatcggctg attctccttc ccgcgattag ccagggctgg
    10321 aggtaggctc ggacctcagg gggcagggga ggtgttccgg ggccgggggt cagttgctcg
    10381 ctcaggccga gccgggagaa gctgcggacg ccttgagccc gcaaactggc gcggtcgagg
    10441 acggtttccg gtgcgtgagc caggatgtgg aaggcccatt cgatctgggc gtacttgagg
    10501 ccgatgggga tataggcttc cccttggcga tccagagttt ccgcggtggg gaagtggaga
    10561 gcctggtcgt tggtgagggg ctccccacgt aaggccaacc gttccatcca tcgcgtggcc
    10621 agcaggaggg ctcgatcgcg ctgggcgggc gggaagccct cccacgcctg ggcgcggttg
    10681 gggtcgtcgg cgaagtactg gtcggcctcg ttgcggccgg catagctgtt ggcggttgag
    10741 ccaccaactt cgatatcgag tggcgtgggc atggggcctc cacgggcaag acgagatggg
    10801 gggccagccc cctctcccca cgggagagca ccggggcgag gggtgccggc cccgttcgag
    10861 cacggaagcc cttctaggcg tccgcgttgt ccagcagcat cgagcagccg aagttcgcgt
    10921 cgatggtgtc gatcccgtac gcggtggtca tgatgacctc atagccccgg agcgaggcat
    10981 cgcgctggat ctcgatgcgc ggatcccact tgatgaccat gccgatggcc tcgcgggaga
    11041 acatggcgca tcggacatcg ccattgacat cctggatcag gttgccggag cggtaggtga
    11101 tgaccccata ggggttcccc acgtagcccg tccggacaat ctcctcggtc acggagtcgc
    11161 cgaactggtt ggtgttgccg gagaggagct ggatcaggtc ctcctcgaca tagggcgaga
    11221 tgaccgcgaa gtagggctcg ggggcattat gggcggccag gagg
//
LOCUS       DAOSPL010000004        11084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_5871_length_11084_cov_7.659353, whole genome shotgun sequence.
ACCESSION   DAOSPL010000004 DAOSPL010000000
VERSION     DAOSPL010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 11084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11084
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5871_length_11084_cov_7.659353"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PLQ54_00155"
     CDS             <1..695
                     /locus_tag="PLQ54_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91698.1"
                     /translation="QWREPGWLAVCGELVIAGRSTQECARRLAVWWGEQGFPRGETQA
                     SVGDIAARDSRLTLQDALTGAGILAGGRVLTHAQEVAAGIELMRQLLRQERVLVAPRC
                     RVVREEFNGYTYRPGAHEGEPERGPRKTLDHTMDALRYLVWHMCRPRRARGLYRDPAD
                     PAGLLMPGDSGVGSVDEQALAEWQNGRRERRGEPPEPVPGPESSGPVAVLDMRAIERR
                     RRGNVVWRVPIR"
     gene            674..1468
                     /locus_tag="PLQ54_00160"
     CDS             674..1468
                     /locus_tag="PLQ54_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91699.1"
                     /translation="MASANQVAVNWSEMVRQAQALEAALVEQTVGKLRAADADKFLSG
                     EGRAARHKEALALYVDVLTQLDAKTDGEMEKGVRAAQAVLDAAVVKTTATERLEQRML
                     AQTLADALPPAELRAELMKALGAGATVQAVVLSEEVPLRLQAMARRGVAGAEAQVVGL
                     AQEVEAWRDRVKPERVAARETVEAIRVARTELQSGRMGRTLYGQRHRAFAALPGHVGT
                     EAGMMHWTRQGSPGGDPQGVGTDAVLSHGVVPGQGQAALARGGTVG"
     gene            complement(1556..2050)
                     /locus_tag="PLQ54_00165"
     CDS             complement(1556..2050)
                     /locus_tag="PLQ54_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91700.1"
                     /translation="MDNADNVGTDAEQLTIEGLATEAHVSSKTLRRLIAKGHLVPDAK
                     ERIGGRRFRYMFNRNQVAHVAHLVHDGASPLSPAQSRPRKPTRQEELDTTRKELDRAL
                     SALSKAEEEARSWREQAQQTMARLAEVQAQNGELIRLALPQPAAPVDTSPAPRRPWWA
                     PWRR"
     gene            complement(2107..2913)
                     /locus_tag="PLQ54_00170"
     CDS             complement(2107..2913)
                     /locus_tag="PLQ54_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HOS91701.1"
                     /translation="MALPNWLHDDDRWFTFLDGVKSTPAWSAYPQEFFARLRDLRGRA
                     RIVGWMILEEGSASTQALQDNGYSHPPRAKQDLQEQGIAVLCTIGYNDRNGRRMAVYS
                     FPSPEAVLKGPRSGRVALPKAFTDHVKAAAHGRCALCAGIFDPTGLQADHRVPYAIVG
                     DDPNSDLRLSEFMAVCRSCNRAKSWTCEHCPNWTDPDPELCATCYWASPTDYTHISTL
                     VIRRLDLTWQGPEIDDFQLLQLLAQQASSPLPEYVKHLLANHIRKGQGSG"
     gene            complement(2853..3824)
                     /locus_tag="PLQ54_00175"
     CDS             complement(2853..3824)
                     /locus_tag="PLQ54_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015112037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOS91702.1"
                     /translation="MFVPQPFPYQGSKRRLAPLILQYFPPGAARLVEPFAGSAAVSLA
                     SACARRTAAFALNDLNSALMELWERIINAPAEISTQYESLWKRQAEREREFYDEVRTE
                     FNRTGQPDLFLYLLARCVKASIRYNSNGEFNQSPDNRRKGMRPGTMRVQLERTSRLLR
                     GRTRLSSVDYREVLAETTSSDIVYMDPPYQGVCGNRDPRYINGVCFEDFAAALDDLSR
                     RGVPFIVSYDGRTGDKAFGRKLPDELNLAHIEVDAGRSSQATLLGSDAITYESLYLSS
                     DLAAKVGVDHLRKGPRPSPQLALFGDGSWPSRTGSTTTTAGSLSSME"
     gene            3934..4065
                     /locus_tag="PLQ54_00180"
     CDS             3934..4065
                     /locus_tag="PLQ54_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91703.1"
                     /translation="MWIAATGSHYHRETCGALHGQGSEISRSVAEAKGYAPCSLCRP"
     gene            4210..4392
                     /locus_tag="PLQ54_00185"
     CDS             4210..4392
                     /locus_tag="PLQ54_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91704.1"
                     /translation="MRPPAEEGWVTVTFRLRPALRQALQDESYATNTTQQEIVEEALR
                     RELDRRAAARAPVAAE"
     gene            4412..4591
                     /locus_tag="PLQ54_00190"
     CDS             4412..4591
                     /locus_tag="PLQ54_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91705.1"
                     /translation="MNLDEDPENADWPKRTWDLPTDMDEVRALLRAWGMTVEEFKRLP
                     VYRWNVDKVPGLKDL"
     gene            4622..5260
                     /locus_tag="PLQ54_00195"
     CDS             4622..5260
                     /locus_tag="PLQ54_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91706.1"
                     /translation="MRVLNMATDTEHEATLLGTRRATPYRAIPAVDGEAVDPLQWALY
                     RVVSATDEELAAFQAAGIPCHRLTTEEADELAARDPDTFDAEDEAERWMAAAWADLRA
                     HDPTLPELSVVAEALESAAEVGAVPRETTVTLRNLFHGTAVQVRCPEGLPYLLPRAEY
                     LRVRKRLCGVPKCRCGLVSGVQEVGIEWVLPDRGEFTWQLHAYPRHPTNEGL"
     gene            5610..8171
                     /locus_tag="PLQ54_00200"
     CDS             5610..8171
                     /locus_tag="PLQ54_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91707.1"
                     /translation="MPATTLQIPSDTIPSALVAEPRWLCWRYKTQSDGKVTKVPYRAR
                     ARGLPRKPDGSSTNPASWCDYQTAVAAVAANPKLDGLGFALGDGFVGVDLDHCIDSET
                     GEVAEWAQAIVGLLDSYTEVSPSGQGLHVIVRGSVTEDRRKRVGMPGGGALEVYAHGR
                     YFTVSGRRFGAREAVEERSAALATLVAWHLALPSKAPKEPQESSAPRVDTHTDFPRQR
                     HGALLEHDERYKATWEHERPDLSDQSLSTYDLALCSLAAQYGWTADELAALIRTHRAE
                     HGETEKARRVDYVQGTVAKALAGVKARPAGDHDKGGRLVPLPEGAPARPEINVSNKQP
                     VELTDEALRVLHATNEPPELFVRAGELVRVRADEKGRPVIDTANEKIVFERLTAVADW
                     LKTLPPRKNQPEPPTLASRPPEWLPGNVIARGRWPFPALEGIAEAPYLRPDGSVMARA
                     GYDPITLLLYKPLPNLVVPPIPQRPTRDDVVNALMVAMEPLAEFPFIDDASRANALAM
                     LLSPIVRPAVDGPVPLALSDAPQPGTGKSLLTEIVSIVATGREAAMISAPETEAEWAK
                     SINAILCTGATVVVIDNVETTIFAPSLAKSISGSFSAKRQLMQDTKLTLLPQRACWFA
                     TGNNLRPRLKGALPRRCYWARQDAQMAQPWTRDSQNFRHPEIKSWTKANRGAILAALL
                     TLARAWFEAGCPAPKAAPKLGGFEAWRRVVGGILEFAGVPDFLANLAELWAADQDVAE
                     WEPFLRALHARRGSNPFTVADLARDLVHEQALAGVVRSAPGSAVPDEDAGTLPSLVAH
                     VPPDLLDRDGRLNHRSLGRALARLDGTRHGSDDIRLERAGTHRLTNTVLWKVVVG"
     gene            8542..9315
                     /locus_tag="PLQ54_00205"
     CDS             8542..9315
                     /locus_tag="PLQ54_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOS91708.1"
                     /translation="MARPRGSKKAKQQQERLLTGGGKLVGYIRVSTEGQQTNGHSLAG
                     QEQRLQEACERAGFELVTVQHDVASGAKSDRDGLAEAQAMVEQGLAEGLVFAKLDRVS
                     RSLLHSAKLVEWARAGGFTMLSADEGPMIARGQLVNEALPFFQALAQVERERISRRTR
                     EGLAAARAKGVALGCPVRRACGDPAVQRAIALRRQGLTLQAIADRLARDGVRGARGGN
                     LTPAAVWGWVERCAPELNQPFGLSRGGAVAQGSEDGAAP"
     gene            9603..10127
                     /locus_tag="PLQ54_00210"
     CDS             9603..10127
                     /locus_tag="PLQ54_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91709.1"
                     /translation="MVMPLTKRTNGLIAKAVRMRWETVLIGGAPRSYESIAQALGVTE
                     RTLRRWRHTEAWADEVAAMDCPEQERELINQAKAALRYLLMDQDHKIRLAAARAVLDY
                     CLARKLEVEHHGEVVYLRRYDVSGVSSETLHQWITEATAAEGIGPGAERALPESVRVE
                     VVDGEPCEAVEADG"
     gene            10120..>11084
                     /locus_tag="PLQ54_00215"
     CDS             10120..>11084
                     /locus_tag="PLQ54_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOS91710.1"
                     /translation="MAEQVLPPPLHSGTERLRFLRETAREAAQAGDVEAVFRCLGYKP
                     HAGQRAFHEARGYPRRVICTGRRFGKTLAAAMEACTTLLRGDNRGRGATRTLVLCPVA
                     ELSERIFRQVRTSLCRQLAFRPERLYDTGQRRELVMPWGSSLVCRSAAGEESVLGEGF
                     DLALLDEAARLPPQVWEAVVEPALADRNGAAVFLSSPLAFGWFSGLWARCGNADWPEW
                     WGYRGKTEENPAISRAWLAERQRTVSPEVWRREYCGEFVNLEGTVYPEWSELVHVSPE
                     AEFDRRYPPMLTFDFGTTEASPFVCLVCQWREPGWLAVCGELVIAG"
BASE COUNT         1788 a         3418 c         4074 g         1804 t
ORIGIN      
        1 gccagtggcg ggagcctggg tggctggcgg tgtgtgggga gctggtcatc gcggggcgat
       61 cgacgcagga gtgtgcgcgg cggcttgccg tgtggtgggg ggagcagggg ttcccccggg
      121 gcgagacgca ggcgagtgtg ggcgacatcg ctgcccgaga cagccggctg accctacagg
      181 acgcgttgac cggtgccggg atcctggctg gcggccgggt gctcacccac gcgcaggagg
      241 tggccgcagg catcgagctc atgcggcagt tgctgcggca ggagcgggtc ttggtggctc
      301 cccgttgccg cgtggtgcgc gaggagttca acggctacac ctaccgcccc ggagcccacg
      361 agggcgagcc cgagcggggc cctcgcaaga ccctggacca cacgatggat gccctgcggt
      421 acctggtgtg gcacatgtgc cggccccggc gggctcgggg cctttaccgc gatcccgcgg
      481 acccggcggg tctcctgatg ccaggggata gcggcgtggg ctctgtggac gagcaggcgt
      541 tggcggagtg gcagaacggg cgtcgggagc ggcgcggaga gcccccggag ccggtgccgg
      601 ggccggagtc atcagggcct gtcgcggtcc tggacatgcg cgcgatagag cgcaggagga
      661 gaggcaatgt cgtatggcga gtgccaatca ggtagcggtg aactggtcgg agatggtgag
      721 gcaggcgcag gcgctggagg cggccctggt ggagcaaacg gtgggcaagc tccgggcggc
      781 ggacgcggac aagttcttga gcggcgaggg ccgggcggcg cggcacaagg aggccctggc
      841 gctttacgtg gacgtcttga cgcagttgga cgcgaagacg gatggggaga tggagaaggg
      901 ggttcgagcg gcccaggcgg tgctggacgc ggcggtagtg aagaccacgg cgacagagcg
      961 gcttgagcag cggatgctgg ctcagacgct ggcggacgcg ctgcccccgg cggagctgcg
     1021 tgccgagctg atgaaggcgc tgggggcggg agcgacggtg caggcggtgg tgttgtcgga
     1081 ggaggtgccg ttgcgcttgc aggccatggc ccggcgtggc gtggcgggtg cggaggccca
     1141 ggtggtgggt ctggcgcagg aggtggaggc gtggcgcgac cgggtgaagc cggaaagggt
     1201 ggcggcccgt gagacggtgg aggccattcg cgtggcgcgg acggagcttc agtcggggcg
     1261 gatggggcgg acgctatacg ggcagcggca tcgggcgttc gcggcgttgc cggggcacgt
     1321 ggggaccgag gcggggatga tgcactggac gcggcagggg tccccggggg gtgacccgca
     1381 gggggtcggc acggacgcgg tgctgtctca cggtgtggtg ccggggcagg ggcaggccgc
     1441 cctggcgcgg gggggaacag tggggtaggg gtgcccccgg ggtgctggcg tggtgtgggt
     1501 gacgggcctc tgttctctcc tcgtggaggg ggcgggggcc cgttgttgtg tggggctacc
     1561 ggcgccaggg ggcccaccag gggcggcgcg gggcaggtga ggtgtccaca ggggcggcgg
     1621 gctggggcaa ggccaggcgg atgagctcgc cgttctgggc ttggacctcg gctaggcggg
     1681 ccatggtctg ttgggcttgt tcccgccagc tccgggcttc ttcctcggct ttggacaagg
     1741 cggacaatgc ccggtccagc tctttcctgg ttgtgtccag ttcctcttgt ctagttggtt
     1801 tgcggggccg tgactgagct ggactaagcg gacttgctcc gtcgtggaca agatgtgcca
     1861 cgtgtgcgac ctggttccgg ttgaacatgt atcggaagcg gcggccaccg atacgttcct
     1921 tggcgtccgg caccaggtgg cccttggcaa tcaatcgccg aagcgtcttc gagctcacgt
     1981 gggcttccgt ggccaggccc tcgatggtga gctgttctgc gtccgtgccc acgttgtccg
     2041 cgttgtccat gatggccgga tgttcgtgtg gggcgcgggg cggtcctgct ggcacaggat
     2101 aggcgcctat ccggagcctt ggcctttgcg gatgtggttt gcgaggagat gtttgacata
     2161 ctccggtagc ggagaggagg cttgttgagc caagagctgt aggagctgga agtcgtcgat
     2221 ctcaggccct tgccaggtga ggtccaagcg gcggatgact agtgtggaga tgtgggtata
     2281 gtccgtgggg cttgcccagt agcaggtggc gcaaagctcg gggtccggat cggtccagtt
     2341 ggggcagtgt tcgcaagtcc aggactttgc tctgttgcac gagcggcaga ctgccatgaa
     2401 ctccgagagg cggagatccg agttcgggtc gtcgccgacg atcgcgtatg gaacgcgatg
     2461 atcggcttgg aggcctgtgg ggtcgaagat gccagcacac aaggcacatc tgccatgagc
     2521 tgccgctttc acgtggtcgg tgaaagcctt gggcaacgcc acgcggcctg accgaggccc
     2581 tttgagaacg gcttcaggcg aggggaatga gtagacggcc atacgccggc cgtttctgtc
     2641 gttgtagcct atcgtgcaga ggaccgcgat gccctgttct tgcagatcct gcttggctcg
     2701 cggggggtgg ctgtaaccgt tgtcttgaag tgcttgtgtg ctggctgacc cctcttccaa
     2761 gatcatccaa cccacgatgc gagcgcggcc gcgaaggtcc ctgagcctcg caaagaactc
     2821 ctggggatag gccgaccaag cgggggtgct cttcactcca tcgaggaaag tgaaccagcg
     2881 gtcgtcgtcg tggagccagt tcgggagggc cacgatccat ctccgaagag agcaagttgg
     2941 gggcttggtc gcgggccctt ccgcaggtgg tccacgccga cctttgcggc caagtctgag
     3001 gacaggtaca gagactcgta cgtgatggcg tcactgccga gaagagtggc ctgggaagac
     3061 cgcccagcgt ctacctcgat atgtgccagg ttcaactcgt ccgggagctt ccggccaaac
     3121 gccttatctc ctgtccggcc gtcatagctc acaatgaacg ggactccccg ccgtgatagg
     3181 tcatccagag cagcggcgaa atcctcgaag cacaccccgt tgatgtaccg cgggtctctg
     3241 ttaccacaga ccccttggta tggcgggtcc atgtagacga tatcggagct cgtggtctct
     3301 gcgagaacct cgcggtagtc cacagaagac agccgcgttc gcccacggag aagacgggaa
     3361 gtcctctcca gttgcactcg cattgtgcct gggcgcatac ccttccggcg gttgtccggg
     3421 ctttggttga actcgccatt tgagttgtac cggatggatg ccttgacaca ccgcgccaac
     3481 aggtagagga acagatccgg ttgccccgtc cggttgaact cggtccggac ttcatcgtag
     3541 aactcgcgct cccgttctgc ttggcgcttc cacagggact cgtattgggt cgagatctcc
     3601 gcaggagcat tgatgatccg ctcccacagc tccatgagtg ctgagttcaa gtcgttcaga
     3661 gcaaatgcag cagtcctgcg ggcacacgcg ctagcaagcg aaacggctgc cgaaccggcg
     3721 aaaggttcga ccaatcgcgc tgcgcctggt gggaagtact gcaagatcag cggagccaac
     3781 cgccgcttgc taccttggta tgggaacggt tgcggaacaa acactcgttc gtcctccttt
     3841 catggcccta ttcccccgat ctccaagtcg cccttcccga ccggcagaag atcctcgagg
     3901 aagccggcaa aaggcgacga gacccgtggg gtcgtgtgga tcgccgccac cgggagccac
     3961 taccaccggg agacctgcgg cgccctccac gggcagggct cggagatctc gcgttcggtg
     4021 gccgaggcca agggctacgc cccgtgttcg ctctgccggc cgtagtggcc ccctctacgg
     4081 gcaccccccg ggccggccct cgcggcagcg gccgttctgg cgctgaagct tgcaagcgtg
     4141 caagcccagt ggtatcttgt aggggcccct ggtgtggttg ggggcctcaa gacggcgagg
     4201 gggttcacga tgcgaccgcc agcggaagag ggctgggtga cggtgacgtt tcggctccgg
     4261 ccggcgttac ggcaggccct ccaagatgag agctacgcca cgaacacgac ccaacaggag
     4321 atcgtcgaag aggccctgcg gcgagagctt gaccggcggg ccgcggcaag ggccccggtg
     4381 gcggccgagt agggcgaaag gcggctccca agtgaacctc gacgaggacc ccgagaacgc
     4441 ggactggccg aagcgcacct gggacctgcc gaccgacatg gacgaggtgc gggcgctgct
     4501 ccgggcctgg gggatgaccg tcgaggagtt caagcggctg cccgtctacc ggtggaacgt
     4561 ggacaaggtg cccgggctga aagacctgtg agtggtgccc cggacgggga gatgaggcgc
     4621 gatgcgcgtt ctgaacatgg caacagacac ggagcacgag gcaaccctgt tgggcacgag
     4681 gcgggccacc ccctaccgcg cgatccccgc cgtggacggc gaagccgtgg atcctctgca
     4741 gtgggcgctc taccgggtgg tgtcggccac ggacgaggag cttgccgcgt tccaggcggc
     4801 cggcatcccc tgccaccggc tgaccacgga agaggcggac gagctggcgg cccgagaccc
     4861 cgacaccttc gacgccgagg acgaagccga gcggtggatg gcagcggcgt gggcggacct
     4921 gcgggcccat gaccccacac tgcccgagct gagcgtggtc gccgaggccc tggagagcgc
     4981 ggcggaagtc ggggctgtcc cacgggagac caccgtcacc ctccgcaacc tgttccacgg
     5041 cacggccgtg caggtgcggt gccccgaggg actgccctac ctactgcccc gggccgagta
     5101 cctgcgcgtc aggaagcggc tctgcggcgt gccgaaatgt cggtgcggcc tggtctcggg
     5161 cgtgcaggag gtcgggatag agtgggtgct gcccgaccgt ggcgagttca cctggcagct
     5221 ccatgcatac ccacggcacc cgaccaacga ggggctctag ccgaaagtcg tggcgaagaa
     5281 tcgggtgcgg ccggtagttg tgacatggga aggctgaagg ggcgggtccg ccgctcccga
     5341 aaatgcccca tacaacgagc gcgcccggag gccggcacac ctccaggcgc tagagcgagt
     5401 agagcatatg agctttgtgg cgcgggctgt ggcaacagcc catacaactc acgattgtag
     5461 cctacgagat tcgtcggcac aatcttcgac gcgcttttcg cggcaccttt cgacgaccgt
     5521 ttttgtccca aaaactcgct cgctcgtttt ccgtccgcgg gccgtggtgc cggccacagc
     5581 ccgccgtgat ccacgacgag gagcgagcga tgccggcgac cacactacag atcccttctg
     5641 ataccatccc ttcggcccta gtcgcggagc cgcgttggct ctgctggcgg tataagaccc
     5701 agtcagacgg caaggtcacg aaagtcccct accgggcacg ggcgagaggg ctgcctcgca
     5761 agcctgacgg ctcgtcgacc aaccctgcct cttggtgcga ctaccagacc gccgtggccg
     5821 ccgtggccgc gaacccgaag ctcgatggcc tgggcttcgc gcttggcgac ggcttcgtgg
     5881 gcgtggacct cgaccactgc atcgactccg agaccggcga agtcgccgag tgggcccaag
     5941 ccatcgtcgg actgctggac agttataccg aggtgtcgcc cagcggccag ggcttgcatg
     6001 tcatcgtgcg gggcagcgtc actgaggacc gccgcaagag agtcgggatg cccggcggcg
     6061 gggcgctgga ggtctacgcg cacggccggt atttcaccgt tagcggccga cgctttgggg
     6121 ctcgtgaggc cgtcgaagaa cgctccgctg ctctggcgac cctggttgcg tggcacttgg
     6181 ccttaccgtc caaggcgcca aaagagcccc aggagagctc tgctccccgc gtggacacgc
     6241 acaccgactt cccccgacaa agacacggcg cgctcctcga gcatgatgag cggtacaagg
     6301 ccacgtggga gcacgaacgg cccgacctat ccgaccagtc tctgtccacc tatgacttgg
     6361 ccctctgctc gttggcggcc caatacggct ggacggccga cgagctggcg gcgctgatca
     6421 ggacccaccg agcggagcac ggagaaacag agaaagcgcg gcgggtggac tacgtacagg
     6481 gcacagtggc caaggccctc gccggcgtga aggctaggcc ggcgggcgac cacgacaagg
     6541 ggggtcgcct cgtgccgctt cccgagggtg ccccagcccg gcctgaaatc aacgtctcga
     6601 acaagcagcc agtcgagctc acggacgagg ccctgcgcgt ccttcacgcg acgaacgagc
     6661 cgcccgagct attcgtgcga gccggcgagc tcgtgcgggt acgagccgac gagaagggtc
     6721 gcccagtgat cgacacggca aatgagaaaa tagtgtttga gagactcacg gccgtggccg
     6781 actggctgaa gaccttgccg ccccggaaga accagcccga gcccccaacg ctcgcttcgc
     6841 gccctccgga gtggctgccc gggaacgtta tcgcccgggg ccggtggccc tttcccgcac
     6901 tagagggcat cgcggaggcc ccatatctgc ggccggatgg gtccgtcatg gctcgagccg
     6961 gctacgaccc gattacgctg ctactttaca agccgctccc gaacctcgtc gtgccgccga
     7021 tcccccagcg ccccacgcgc gatgacgttg tcaacgctct gatggtcgcc atggagccgc
     7081 tggccgagtt ccccttcatc gacgacgcga gtcgggcgaa tgccctggcg atgctgctct
     7141 cgcccatcgt ccggcccgcc gtcgacggcc cggtgccatt ggcgctcagc gatgcgccac
     7201 agccggggac ggggaagtcg cttctgaccg agatcgtgtc catcgttgcg acgggccgcg
     7261 aggccgcgat gatctcggcg ccagagaccg aggctgaatg ggccaagtcg atcaacgcca
     7321 tcctctgcac gggcgccaca gtggtggtca tcgacaacgt cgagaccacg atcttcgcgc
     7381 cttccctcgc caagtcgatc tctggctcct tctccgccaa gcgccagctt atgcaggaca
     7441 ccaaactgac actcctgccg cagcgggcgt gctggttcgc aaccggcaac aacctccgac
     7501 cgcggctgaa gggagcgctg cctcgccgat gttactgggc gagacaggat gcacagatgg
     7561 cgcagccgtg gacccgcgac agccagaact tccgccaccc ggagatcaag tcatggacga
     7621 aggctaatcg cggcgcaatc ctcgcggcgc ttctgacact ggctcgagct tggttcgagg
     7681 ccggctgccc tgctccaaag gccgctccga agctcggggg tttcgaggct tggcggcgcg
     7741 tcgtgggcgg gatcctcgaa ttcgccggtg tgccggactt cctcgcaaac ctcgccgaac
     7801 tgtgggccgc agaccaggac gtggctgaat gggaaccctt tctccgggcc ctccatgccc
     7861 ggcgaggctc caaccctttc acagtggccg acctcgctcg agacctcgtt catgagcagg
     7921 cgctcgccgg ggtcgtcaga tccgccccag gcagcgccgt ccccgatgaa gacgccggca
     7981 ccttaccttc actcgtggcg cacgtgccgc cggatcttct cgatcgcgac ggccggctga
     8041 atcatcgttc gctgggccgg gcgttggccc gtctcgacgg cacgcgacac ggcagtgacg
     8101 acattcggct tgagcgggcc ggtacccaca gattgacgaa caccgttctc tggaaggtag
     8161 tggtcggctg atcgcccgcc gggtttccgg gtttgccggg tttccctgca ttctaccagg
     8221 gtcttacgcg cgcgcgcgcg tatacacaca cgcgcgtaac accccgcctt aaatagtgga
     8281 gaacccggaa acccggaaac ccggcgagtc accgggggct cgggcaagca gttgtgggtc
     8341 gctgtcgccg gcccatcccc cccggttgtg accggacgtt gggccccttc cggggccccc
     8401 cgagtgtgac cgggggggcg cggccctctg gaaagggccc ctgggtgtgc ccgaaacacc
     8461 ctgcgtttat gcttgacgga cgtttacgat aaatggtacc ctgttggcgg cactctagag
     8521 gcaccagggg aggcggccac tgtggccagg ccaaggggtt ccaagaaggc gaagcaacag
     8581 caggagcggc ttctgacggg agggggcaag ctcgtgggct acatccgcgt ttccacggaa
     8641 gggcagcaga ccaacgggca ctccctggcc gggcaggagc aacgactgca ggaagcgtgt
     8701 gagcgggccg gcttcgagct ggtaacggtg cagcacgacg tggccagcgg ggcgaagagc
     8761 gaccgcgacg ggctggccga agcgcaggcc atggtcgagc agggcttggc cgagggcctg
     8821 gtgttcgcca aactcgaccg cgtgagccgt tcgctactgc actcggcgaa gctcgtggag
     8881 tgggccaggg ctggcggctt cacgatgcta agcgccgacg agggccccat gattgcccgg
     8941 ggccaactgg tcaacgaggc cctgcccttc ttccaggcgc tggcccaggt cgagcgggag
     9001 cgcatcagcc ggcggacacg tgaggggctg gcggcggccc gggccaaggg cgttgccctg
     9061 gggtgccccg tgcgccgtgc gtgtggcgac ccggctgttc agcgtgccat cgccctgcgg
     9121 cggcagggcc tgacgttgca ggccatcgcc gataggctgg cgcgtgacgg ggtgcggggt
     9181 gcccggggcg gcaacctgac gccggcggca gtgtggggct gggtggagcg gtgtgccccg
     9241 gaactgaacc agccgttcgg cctttctcgc ggcggagcag tggcccaggg ctccgaggac
     9301 ggggcagcgc cgtgagtgtg ccacgtaagg tactgggaca gcagacgcca agcacctggg
     9361 gccttctggg ggccttggtg gaggtgtgct gcccggtgtg cgatcgggtg cattgcgagg
     9421 cttcggccga tacccggctg atccgttggc ggtgccggcg gtgcggccgg tggtgtatgt
     9481 ggaagggtga ccgggcggcc tagcggccga cccccgacat acggcaacag cagcgcccca
     9541 cgcgggccgg cgacctgggc gaccaggcga ccggcccgtt cgcgtgtacg acgaagggag
     9601 cgatggtgat gccactgacc aagcgcacca acggactgat cgccaaggcg gtgcgtatgc
     9661 ggtgggagac cgtgctgatc ggcggtgctc cgcggtcgta tgagtccatc gcccaggccc
     9721 tgggcgtgac ggaacgcacc ctgcggcggt ggcggcacac ggaggcatgg gccgacgagg
     9781 tagcggccat ggactgcccc gagcaggagc gcgagctgat caaccaggcc aaggcggctc
     9841 tgcgatacct gctgatggac caggaccaca agatccggct ggcggcggcc cgggcggtgc
     9901 tcgactactg cttggcgcgg aagctggagg tcgagcacca cggcgaggtg gtgtacctcc
     9961 ggcgctacga cgtgtcgggc gtgtcttcgg agacgctgca ccagtggatc acggaggcga
    10021 cggcggccga gggtatcggc ccgggtgcgg agcgtgccct gcccgagtcc gtccgtgtgg
    10081 aggtagtgga cggcgagccc tgcgaggcgg tggaggcaga tggctgagca ggtgctgccg
    10141 ccaccgttgc actcgggcac cgagcggctt cgcttcctgc gcgagacggc acgcgaagcg
    10201 gcccaggcgg gcgatgtgga ggctgtcttc aggtgcctgg gctacaagcc ccacgcgggc
    10261 cagcgggcgt tccacgaggc acgaggctac ccgaggcgag tcatctgcac gggccggcgg
    10321 ttcggcaaga ccttggcggc ggcgatggag gcttgcacca cgttgctgcg cggcgacaac
    10381 cgcgggcggg gtgccacgcg caccctggtg ctatgccctg tggccgagct gtccgaacgc
    10441 atcttccgcc aggtgcgaac ctcgttgtgc cggcagctcg ccttccggcc tgaacggctc
    10501 tacgacacgg ggcagcgccg tgagctggtg atgccctggg gctcgagctt ggtgtgtcgg
    10561 tctgcggccg gtgaggagtc ggtgctgggc gagggcttcg acctggcctt gctcgacgag
    10621 gcggcccggc tcccgcccca ggtgtgggag gccgtggtcg agccggccct ggcggaccgg
    10681 aacggagcgg cggtgtttct gtcgtcgccg ctggcgttcg ggtggttctc gggcctctgg
    10741 gcccggtgtg ggaacgcgga ctggccggag tggtggggct accggggcaa gacggaagag
    10801 aaccccgcga tcagccgggc ctggctggcg gagcgccagc gcacggtgag ccccgaggtc
    10861 tggcgtcgtg agtattgcgg cgagttcgtc aacctcgagg gcacggtgta tcccgagtgg
    10921 tcagagctgg tccacgtctc gccggaggct gagttcgacc ggcgataccc cccgatgctc
    10981 accttcgact tcggcacgac ggaagccagc ccgttcgtgt gtttggtgtg ccagtggcgg
    11041 gagcctgggt ggctggcggt gtgtggggag ctggtcatcg cggg
//
LOCUS       DAOSPL010000005        10211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_6560_length_10211_cov_3.528555, whole genome shotgun sequence.
ACCESSION   DAOSPL010000005 DAOSPL010000000
VERSION     DAOSPL010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 10211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10211
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6560_length_10211_cov_3.528555"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLQ54_00220"
     CDS             <1..513
                     /locus_tag="PLQ54_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HOS91711.1"
                     /translation="SASGVSGMGRKPSLPGHFPECNEDARAYGIGIHRHTPEMEQELG
                     RLTSEPLKVLFVPHLIPVSRGIVTTAYAPLRRECTTADLLEAYREFYRGEPFVRVRGE
                     SESVSLKPIQGTNYCDVGVRADSRTGMAVALASLDNLGKGLSSAAVQCMNVCCGFDET
                     AGLRQPAIWP"
     gene            complement(787..1062)
                     /locus_tag="PLQ54_00225"
     CDS             complement(787..1062)
                     /locus_tag="PLQ54_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91712.1"
                     /translation="MPDDELTPLRIGELLSGAPPPRVAATVADLADLVAACVAAQDGT
                     EALRILAEYRALESQLPTEWRPQCGASGAPAAGVSSPGPGRRPLERV"
     gene            1359..3695
                     /locus_tag="PLQ54_00230"
     CDS             1359..3695
                     /locus_tag="PLQ54_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91713.1"
                     /translation="MAGALDVTAARTAASWHSIESVTATAVPEGVRLEVTDPARSYGN
                     ASLAVTADWTRFLLTVEIASSTGGWYVALSGDEVGFATVAEGRGAGTATVDPGADGSV
                     EGPATLNVHVGASGPSRASVVVRNLSLHPKPSLTIGASDVDLSAGASLTVDIELPDGR
                     PYDGMLLAGVADRPEALGAARQAPGRYAFELPRGLPPAIYAIDFTLPGEPERHERRWV
                     RVRAPVLAVDRLAYQTSPSGIELSLSDAHGQPRTATRLEAVLDGRRVRATAIGRGRWR
                     LAPRRLCPGVHSLTATADQVALSAPIYVSRGDFIRAHGTRFVDAHGAVFAALGVEGGG
                     SIRDGKQPRWSSFTAWPVADDAMLDAHCAYLASCGINVLRVGLNLAEGCLDTGEGVSF
                     PLAARLARFLAALGRHGLRAQLVLYWGPFGSYGFNSRTAPGYAEADDWLAGPRSRRKQ
                     LRFIGDLTRLFAADDRIIAWEIQNELGVSGDMASERPRTEWVHAMRDALRDGDANHPI
                     GISYLPWGSGADVDPLLWARELDCDFFQFHYSVGGEFGAQLRYGQLSGRPFLAGEFGT
                     SRESDECEARITRDCVWLSLLSGAAGCVGWHQDWVVPEEFRLPAALLKRYHWLDEEAL
                     PPARLLPIAEPWGSADEARREAVRLFDAALSFQPVPSGHLAEHPLLRGLALPSPTGRD
                     PFRVTGAYGGLTYASRRIVVAYLCNRASVRQWSGYGGPLEYRDPAPTPLTIEADVPRS
                     WEVEVRSASTGEVIWQGHGQEPCVLESVSEDLVVIARR"
     gene            4031..4966
                     /locus_tag="PLQ54_00235"
     CDS             4031..4966
                     /locus_tag="PLQ54_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HOS91714.1"
                     /translation="MTVGRIERVPLREVWKHEALDFTKWLEQNIDALNEELHRTFASA
                     EREQNAGDFSVDIVAEDESGNVVIIENQLEKSDHDHLGKLITYTATLQAKTAVWIVSD
                     PRPEHVQAVSWLNECDAVAFYLVKVEAVRIGDSEPAPLLTVIVGPPEEAARGGTSKRE
                     LAERHLIRRRFWTQLLDRARGKTKLFAGVGPSHEASISAGAGVSGLSYNYVVMQHDAR
                     VGLYIDLGKGSEKETEARYDALLASREQVEAAFGCPLVWQRLEGKRACRIKHDLPGAG
                     YKDAEERWPEIQDRMIDAMVRFEAAFRPHIAKLKE"
     gene            5367..7844
                     /locus_tag="PLQ54_00240"
     CDS             5367..7844
                     /locus_tag="PLQ54_00240"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOS91715.1"
                     /translation="MARLAVALGVLVAVASARRCDAVTVTRDEMATARSWVSARLEGV
                     RLPAPDPGLEVLENYDSVQKNARFGNPLRLGGKAYTHGLFCHANSRIVVRLPGPGKAF
                     TARVGVDSNEQTVGGRGSVVFSVNVGDAEAFRSPIVREGMDSVAVDVALGGAREFALE
                     VNDSGDGISCDQADWADARIAMADGSTVWLADLPLADTPALLDAEPPFSFTYGGTPSA
                     ELLPTWRLERASSELDRKRVRHTLTYTDPQTGLVVELVAVEYRDFPTVEWTVYLRNTG
                     AADTPILEGIQALDLGLQRGHYGEFTLHHAVGSPCRVDDYGPLTSSLAPGSVTRIGAA
                     GGRPSNSDMPYFSLESPVRQGLIVAVGWPGQWASEWARDGDTGLRVRAGQERTHLALH
                     PGESIRTPLVVLQFWHGDWVRAQNVWRRWMLAHNVPKPMGRLPEPELFGCSSHLFNEM
                     VEANEENQIGCIDRYLEERIPIRHWWMDAGWYPCDGVGWPKTGTWEVDTRRFPRGLRA
                     VSDHAHERGVKTIVWFEPERVHPDTWLYDQHPEWLLGADGGQKLLNLGNAEAREWLTD
                     HVDRLMTDEGIDLYRQDFNMDPLDYWRGNDAADRQGMTEIRYVEGYLAYWDALLERHP
                     RMFIDSCASGGRRNDLETMRRAIPLWRTDYRCEPVGTQCCTYGISMWIPLSGTGAQDV
                     DAYTFRSNMAPFTNCLLDIRNSKLDYDLLRRLTSQWQQVARYCTGDFWPLTPYSTGAD
                     AWMAWQFDRPETGQGAIQVFRRADSPYEALRLKLRGLDPEARYRVRDLDQGGAKTLSG
                     AELMDPGLRMDITAQPGSAVLVYERVE"
     gene            complement(8070..8345)
                     /locus_tag="PLQ54_00245"
     CDS             complement(8070..8345)
                     /locus_tag="PLQ54_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91716.1"
                     /translation="MPDEEWTPQRIAELMSAAPPPRVAAILADLADLVASYVATGDGS
                     EADRICAAYRALEAQLPPEWRPGRPAVGLPKEPPCPPQRRRERPRLV"
     gene            complement(8540..>10211)
                     /locus_tag="PLQ54_00250"
     CDS             complement(8540..>10211)
                     /locus_tag="PLQ54_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91717.1"
                     /translation="SYVAVYGDPLIDPEPDPYPDGLLDRLSRVGVNGVWLHAVLRDLA
                     PGGVAFPEFGHDHERRLANLRSLVSRAQRFGIGVYLYFNEPRAMPTPFFESRPEVAGV
                     EEGGLTALCTSNATVRQWMSDALTHVFREVPGLGGIYAITASENLTNCASHGAWSACP
                     RCQTRSDTEIIAEVVRTLEEGVHRGSPQAEVIVSDWGWKGHGDAREIIAQLPASVMLM
                     SVSEWAKPIERGGIASEVGEYSISSVGPGPRALRHWEAARQAGVRTAAEVQLNNTCEL
                     ASLPYLPVMDLIAEHCHNLAYAGLSGMLVGWTMGGYPSPSFDLARRFDRTPVPETEVA
                     LDEVARDRFGPDGAPHARKAWTRFSRAYREYPFHISVVYTAPVQVGPANPLYAIQTGY
                     RATMWGIPYDDLDTWRGPYPPEVLASQFGRLAAGWEFGVHALEAAVRATPPDRRPEAE
                     GDLRLARAAGLYFRSVANQIRFVMARDALASGGQTLPPERRRGLQMEIRRCLESEIRL
                     ARQLFALAQEDSRIGFEPTCHYFYLPLDLVEKVINCRWLLNHWDVAGR"
BASE COUNT         1633 a         3485 c         3591 g         1502 t
ORIGIN      
        1 agtgcctcag gggtttcggg tatgggccgc aagccatctc tgcccgggca cttccccgag
       61 tgcaacgagg acgctcgcgc gtacggtatc ggcatccacc ggcacacgcc ggagatggag
      121 caggagctgg gcaggctcac atccgagccc ctgaaggtgc tcttcgtccc gcacctgatc
      181 ccggtgagcc gagggattgt caccacagcg tacgcaccgc tgcggcggga gtgcacgacc
      241 gccgatctgc tcgaagccta ccgagagttc taccggggcg agcccttcgt ccgtgtgagg
      301 ggcgagtccg agtcggtgtc tctcaagccg atacaaggga cgaactactg cgacgtcggg
      361 gtgcgggccg acagccgaac ggggatggct gtggcactgg cctcactcga caacctgggc
      421 aaggggctca gcagtgccgc cgtgcagtgc atgaacgtgt gctgcgggtt cgacgagacc
      481 gccggcctcc ggcagcctgc catctggccc taggcgacga cagccttgtc ccccaccggc
      541 acgtagtcgg ggcagtcggc acatcccgtt gcgcgggtgc ctggtcgtcg gccctcgagg
      601 tgccccagac ggcaaacagg caactgcggc gcctggccgg ccgagtcggc gaggggcggg
      661 cgtacccggc gcccggggcg gtagtaccag cagtcagtac ggtactgttc cattgggtga
      721 caccccaggt gttgtagtga tagtcacaac accaacggtg cgactgagcg tctggttcgg
      781 ctgggctcag acgcgctcca acgggcgcct gcctggtccc ggcgaagaca ccccggcggc
      841 cggcgccccg gaggcaccgc attgcgggcg ccattccgta ggcagttggg actcgagcgc
      901 tcggtactcg gcgaggatcc gcagcgcctc cgtgccgtcc tgggccgcaa cgcaggcggc
      961 caccaggtcg gccaggtcag ccacggttgc cgcgactctg gggggagggg cgccagatag
     1021 cagctccccg atacgcagtg gggtcagctc atcatcgggc attcgcgtac gcctccacga
     1081 acggctcgtc gcacgtgtcg acggacgcca accaaaggag acgcctgcct agcctcgcgg
     1141 gctgccgagc agcaggtctg tgcgagcagt ccggcagtga ccagccgctt gtggccccgg
     1201 gatccgcctc ccctgactgt gtgaccgcct acgggccgtg cctgccacgc ctccgttcaa
     1261 gcccccggag gggcggatcg cgccgcgccg ggaggggcgc tgccatcgcc gtcctgttcc
     1321 tcttgctccc cctcggccgt tccgccggct ggggcgcgat ggctggcgcc ctggacgtga
     1381 cggccgcgcg taccgcggcg agctggcaca gcatcgagag cgtgacggcc acggcggtgc
     1441 ccgaaggagt ccgtctggag gtcaccgacc ccgcacgctc ctacggcaac gcgtcactgg
     1501 cggtgacggc cgactggacc cgcttcctgc tgaccgtgga gatcgcttcg tcaacgggtg
     1561 gctggtacgt ggccctctcg ggggacgagg tcggcttcgc caccgttgct gagggaagag
     1621 gtgctggcac agcgacggtg gaccctggcg ccgatgggtc ggtcgagggc ccggccacgc
     1681 tgaacgtcca tgtgggcgct tcgggccctt cgcgcgcgtc ggtcgtggtg cggaacctgt
     1741 ctctacaccc aaagccgtcg ctgaccatcg gtgccagcga cgtggatctc tcggccggcg
     1801 cgtcgctaac cgtcgacatc gagctgcctg acggcaggcc gtacgacggc atgctgttgg
     1861 ccggagtggc cgacagaccg gaggcgttgg gcgcggctcg gcaggcgccg gggcgctacg
     1921 cgttcgagct cccgcgcggc ctgccaccgg ccatatacgc catcgacttc acgctgccgg
     1981 gcgagcccga gcggcacgag cgccgatggg tgcgcgtgcg ggcgcccgta ctagcggtgg
     2041 accgcctcgc ctaccagacg agcccgtctg gcatcgagct gtcgctgtcg gatgcccacg
     2101 gtcagcccag aaccgcaacc cggctggagg ctgtcctcga cggccggcgg gtgcgcgcca
     2161 cagccatcgg gcgagggcga tggcgactgg cgccacggcg gctctgcccg ggcgtgcact
     2221 cgctcacggc cacggccgac caggtggcgc tctctgcgcc gatctacgtg tcgcgcggcg
     2281 acttcatccg ggcgcacggc acgcgcttcg tcgacgcgca cggcgctgtg ttcgctgctc
     2341 tgggggtcga gggcggaggg tcgatccgcg acgggaagca gccgcgctgg agctcattca
     2401 ccgcctggcc tgtggccgac gatgccatgc tcgatgcgca ctgcgcgtac ctggcctcgt
     2461 gtggcatcaa cgtactgcgg gtggggctca acctggccga gggctgcctt gacaccggcg
     2521 agggcgtgag cttcccgctg gccgcacggc tggcgagatt cctcgccgcg ctcgggcggc
     2581 atggcctacg tgcgcaactg gtgctctact ggggcccgtt tgggtcgtac ggcttcaact
     2641 cacggacggc gcccggctac gccgaagccg acgactggct ggccggacca agaagccgcc
     2701 gcaagcagct ccggttcatc ggcgacctga cgcggctctt cgcggccgac gaccggatca
     2761 tcgcctggga gatccagaac gagctcgggg tcagcggcga catggccagc gagcgtccgc
     2821 gcaccgaatg ggtgcacgcg atgcgggacg cgctgcggga cggcgacgcc aatcacccca
     2881 tcggcatcag ctacctcccg tggggctccg gggcggatgt cgaccccctg ttgtgggccc
     2941 gcgagctcga ctgcgacttc ttccagttcc actactcggt gggcggcgag tttggcgccc
     3001 aactacggta cggccagctc agcgggcgac cgttccttgc gggcgagttc ggcacatcgc
     3061 gggagtcgga tgagtgcgag gcgcgtatca cgcgcgactg cgtgtggctg tcgctgctct
     3121 ccggcgccgc ggggtgcgtt ggctggcacc aggactgggt cgtccccgag gagttccgcc
     3181 tgcccgccgc gctgcttaaa cggtaccact ggctcgacga ggaagccttg ccgcccgctc
     3241 gcttgctgcc catcgccgag ccctggggga gtgcggacga agcgcggcgc gaggccgtcc
     3301 gcctgttcga cgcggctctg agcttccagc ccgtcccctc cggccacctc gccgagcacc
     3361 cgctgctccg tggcctcgct ctgccctcgc cgacgggaag agatccgttc cgcgtcaccg
     3421 gtgcctacgg cgggctgacg tatgcttccc ggcgcatcgt cgtcgcctac ctgtgcaacc
     3481 gcgcctcggt gcgccagtgg tcgggctacg gcggcccgct cgagtaccgt gacccggcgc
     3541 cgactcccct gaccatcgag gccgatgtcc cgcgctcgtg ggaggtcgag gtgcggagcg
     3601 cctcgacggg cgaagtgatc tggcaggggc atggccagga gccgtgcgtg ctcgagtccg
     3661 tgtcggagga cctcgtggtg atcgctcggc ggtagaccgc cggacacccc ggggctccgc
     3721 cccgaactcc gccaggggac cagtcccctg gaccccaccc gggtaccgcc cggacggcag
     3781 ccgctctggg tcgccggcgt ggcggccccg cagatcggta cgggggttgg gcgtccgcgt
     3841 ccgacacctc tgcgctggat ctgagtcgcg tgcagatgcc accagcgaag gcgtcagccc
     3901 ctcgaccggc cgtcccctcg ccatccgttg cccgagtgca gggacgctgc ttgccgcgcc
     3961 cgaaccaggc tcttggtcgg ccgatcggcg cctgggccgg ccaaatatgc gcgaacggaa
     4021 gcgatagctg atgacggtgg gcaggatcga gcgagtgccg ctgcgcgagg tctggaagca
     4081 cgaggccctc gacttcacga agtggctcga gcagaacatc gatgcgctca acgaggagct
     4141 gcaccgcacc ttcgccagcg ccgaacggga acagaacgcc ggcgacttta gcgtcgacat
     4201 cgtggccgag gacgagtcgg gcaatgtcgt catcatcgag aaccagctcg agaagagcga
     4261 ccacgaccac ctcggcaagc tcatcacgta cacggccaca ttgcaggcga agaccgctgt
     4321 ctggatcgtg tccgaccccc gccccgagca cgtgcaggcg gtctcgtggc tcaacgagtg
     4381 cgacgccgtc gcgttctacc tcgtcaaggt agaggccgtc cggatcggcg actccgaacc
     4441 ggctccgcta ctgaccgtta tcgtggggcc cccggaggag gccgctcgcg gcggcacctc
     4501 caaacgggag ctggccgagc ggcacctgat ccgcaggcgg ttctggaccc agctcctcga
     4561 ccgggcgagg gggaagacga agctgttcgc gggagtgggt ccgagccatg aggcgtcgat
     4621 ctctgcgggc gccggggtga gtggcctgag ctacaactac gttgttatgc aacacgacgc
     4681 gcgggttggg ctctacatcg acctcggcaa gggctcagag aaagagactg aagcgaggta
     4741 cgacgcgctc cttgcgagcc gggagcaggt tgaggccgcc ttcgggtgcc ccctcgtctg
     4801 gcagcgcctg gagggcaagc gcgcctgccg catcaagcac gacctacccg gcgcgggcta
     4861 caaggacgcc gaggagaggt ggcccgagat ccaggaccgg atgatcgacg ccatggtacg
     4921 cttcgaagcc gcgttccggc cgcacatcgc gaagctgaag gagtagacgg cgggcgcagt
     4981 gaccacgcgc aggcaaggca cggcgaagtc gcgggccagg aggcccgcag gcacgaacag
     5041 ccatgcccag tgccggtggc cgccctcggc ctccacggca gcgcacgcac aaggcggctc
     5101 cggagcgcgg agcgcccgcc ccgcagcagc agaggtcggt gcctcgccac ctcctgagcc
     5161 tgtcgaaggg cgagacgccg cgcgaacccg gcccctcagg acaggaggga tctggggccg
     5221 ggcctctgtg cgaccgtgac ggacggggcg cggcacgcgg cgcgcctaca cgcagggccc
     5281 tcggacgctc aaccgtgcag gcgccgccct gcccgtcacc gaaggcggcg ctcagcaacg
     5341 cagtcctgga tggggcggat agacccatgg ctcggctggc agtagctctg ggggtgctcg
     5401 tggcggtggc aagcgctcgg cggtgcgacg ctgtgacggt gacgcgagac gagatggcca
     5461 cagcccgatc atgggtctcg gccaggctag agggcgtgag gctaccggcc cccgaccctg
     5521 ggctggaggt cctggagaac tacgactcgg tgcagaagaa cgcccggttc ggcaatccgc
     5581 tcaggctcgg gggcaaggcc tacacgcacg ggctcttctg ccacgccaac agccgcatcg
     5641 tggtgcgact gccaggccca ggcaaggcat tcacggctcg cgttggggtc gacagcaacg
     5701 agcagacggt cggaggcagg ggcagcgtgg tcttctcggt caacgtcggc gacgccgagg
     5761 cattccgctc gcccattgtg cgcgagggca tggactccgt ggccgtcgat gtcgctctcg
     5821 gcggcgcgcg ggagttcgct ctcgaggtga acgactcggg cgatggcatc tcgtgcgacc
     5881 aggctgactg ggccgatgcg cgcatcgcga tggccgatgg gagcacggtg tggctggcgg
     5941 acctgcccct ggctgacact cccgccttgc tcgatgccga gccgcccttc tcgttcacct
     6001 acggcgggac gccatcggcc gagctgctgc cgacctggcg cctggagcgt gcctccagtg
     6061 agctcgaccg caagcgcgtc cggcacacac tgacgtacac cgatccgcag acgggcctgg
     6121 tcgtcgagct cgtggcggtc gagtaccgcg acttccccac cgtcgagtgg acggtctacc
     6181 tgcggaacac cggcgccgcg gacacgccga tcctcgaggg catccaggcc ctcgacctgg
     6241 gtctgcagcg agggcactac ggcgagttca cgctgcacca cgcggtgggc agcccgtgcc
     6301 gggtcgacga ctacgggcct ctgacatcat ctcttgcgcc ggggagcgtc acgcgcatcg
     6361 gtgcggcagg cgggcggcca tcgaacagcg acatgcccta cttcagcctc gagtcgccag
     6421 tccgacaggg actcatcgtg gctgtgggtt ggccggggca gtgggcctcc gagtgggccc
     6481 gcgacggtga caccgggctg cgcgtgcggg ccgggcagga acgcacgcac ctggctctgc
     6541 acccggggga gtcgatccgc acgccgctgg ttgtcctcca gttctggcat ggcgactggg
     6601 ttcgcgccca gaacgtctgg cgccggtgga tgctggctca caacgtgccc aagccgatgg
     6661 gccggttgcc agagcccgag ctgttcggct gcagctcgca cctcttcaac gagatggtcg
     6721 aggcgaacga ggagaaccag atcgggtgca tcgaccggta cctcgaggag cgcatcccga
     6781 tccgccactg gtggatggac gcgggctggt acccgtgcga tggcgtgggc tggccgaaga
     6841 cgggtacgtg ggaggtggac acgcgtcggt tcccgcgcgg gttgcgggcc gtcagcgacc
     6901 acgcgcacga gcgcggtgtg aagaccatcg tctggttcga gcccgagcga gtccacccgg
     6961 acacgtggct ctacgaccag catcccgagt ggctgcttgg ggctgacggc gggcagaagc
     7021 tgctgaacct gggcaacgcg gaagcccggg agtggctcac cgaccacgtc gaccggctga
     7081 tgaccgacga gggcatcgat ctctaccgcc aggacttcaa catggacccg ctggactact
     7141 ggcgcggcaa cgatgcggcc gatcggcagg gcatgaccga gatccgctat gtcgagggct
     7201 acctggccta ctgggacgcg ctgcttgagc gccacccgcg gatgttcatc gactcgtgcg
     7261 catcaggcgg gcgccgcaac gacctggaga cgatgcggcg ggccatcccg ctgtggcgga
     7321 cggactaccg ctgcgagccg gtgggcacgc aatgctgcac gtacggcatc tcgatgtgga
     7381 tcccactgtc gggcacgggc gcgcaggacg tagacgccta cacgttccgc agcaacatgg
     7441 cgccgttcac gaactgcctg ctcgacatcc gcaacagcaa gctcgactac gacctgctcc
     7501 ggcggctcac gtcgcagtgg cagcaggtcg ctcggtactg cacgggcgac ttctggccgc
     7561 tgacgccgta tagcacgggc gccgacgcgt ggatggcgtg gcagttcgac cggccggaga
     7621 ccggccaggg cgccatccag gtgttccggc gggcagacag cccctacgag gcactgcgcc
     7681 tcaagctgcg cggcctggat cccgaggcgc gctaccgcgt gcgtgacctg gaccagggag
     7741 gcgccaagac gctgtcaggc gccgagctca tggaccccgg ccttcggatg gacatcaccg
     7801 cccagccggg gtcggccgtg ctcgtatacg agcgggtgga gtaggggcga ccggcaggtc
     7861 gcccgccgca agggcccggt aacgatcgaa caccgcgggg ctccgcgcgc ggcccctggc
     7921 cggccgagtc ggcgaggggc gggcgtaccc cgcggcctgg gcggtagcac cagcagtcga
     7981 ttcggtgagc atccgtggcc actagccata agtgttgcaa taatagtcac aacaccaact
     8041 caagcatgaa gtggccggtt cagtgtctct cacacgagcc ggggccgttc tcgacgcctt
     8101 tgtggcggac agggtggctc cttcgggagc cccacggccg gccgaccggg acgccactcg
     8161 gggggaagct gcgcctcaag cgcccggtag gccgcacaga tgcgatcggc ctcggagccg
     8221 tccccggtcg ccacatacga cgcgacgagg tcagccaggt ccgccagaat ggccgccact
     8281 cgcggaggag gggcagcgga catcagttcg gcgatgcgct ggggagtcca ctcttcatcg
     8341 ggcattcgcg cacacctcca gggagaccgt ttggcgcgtg tcagcggatg ccccccagga
     8401 aacagcccgt gcctggccct gcgtctgcgg cgaccccagg ccggcctccg ctctcagcgg
     8461 acggcggcgc gaaccaggaa cgatgtcctc cccccccatt cccaagtata ccactggtgc
     8521 aacagggacg cagcacccat tagcggccgg ctacatccca gtggttgaga agccaccggc
     8581 agttgatgac cttctcaacc aggtcgagtg gcaggtagaa gtagtggcac gtgggctcga
     8641 agccgatgcg cgagtcctct tgcgccagcg caaagagctg gcgcgccagc cggatctcgg
     8701 attcgagaca gcgccggatc tccatctgga gcccacggcg ccgctccgga ggcagcgtct
     8761 ggccgccgga cgccaacgca tcgcgggcca tgacgaagcg aatctggttg gccacggagc
     8821 gaaagtacag cccggccgcc cgggccaggc gcaggtcccc ttcggcctcc ggccgccggt
     8881 ccggaggcgt cgcgcgaacc gcggcctcga gtgcgtgcac accgaactcc cagccggccg
     8941 caagtcggcc aaactgcgag gcgagaacct ctggagggta cggcccccgc caggtatcca
     9001 ggtcgtcgta tgggataccc cacatggtgg cccggtagcc tgtctggatg gcgtacagag
     9061 ggttcgccgg gcccacctgg actggggccg tgtagaccac gctgatgtga aacgggtact
     9121 cccggtaggc tcggctgaac cgcgtccatg ccttgcgggc atggggcgcc ccgtcaggac
     9181 cgaaccggtc gcgagccacc tcatcgagtg cgacctcagt ctcgggcacc ggggtgcgat
     9241 cgaagcgccg agcgaggtcg aagctcggcg acggatagcc gcccatggtc cagccaacaa
     9301 gcatcccgct aagccccgca taggccaggt tgtggcagtg ctcggcgatg aggtccatga
     9361 ccggcaggta aggcaaactg gcgagctcgc aggtgttgtt gagctggacc tcggccgccg
     9421 tcctcacacc ggcctgccgg gcggcctccc agtggcggag ggcccgcggc cccggcccaa
     9481 ccgatgagat cgaatactca cccacttcac tggcaatccc gccacgctca atgggcttgg
     9541 cccactcgct gaccgacatg agcatgaccg aggcgggcaa ctgcgcgatg atctcccggg
     9601 catcaccgtg gcccttccag ccccagtccg agacgatcac ctcggcctga ggactaccgc
     9661 ggtggacgcc ctcctcgagg gtccgcacaa cctcggcaat gatctcggtg tcgctgcgcg
     9721 tctggcaccg cgggcacgca ctccaggcgc catgggacgc gcagttcgtc agattctccg
     9781 acgcagtgat ggcatagatg ccgccaagac cgggaacctc ccggaagacg tgggtcaacg
     9841 catccgacat ccactggcgc accgtggcgt tcgacgtgca cagagcggtg agcccgccct
     9901 cctccacgcc ggcaacctct gggcgtgatt cgaagaaggg cgtgggcatc gcgcgcggct
     9961 cgttgaagta gaggtagact ccgatgccga aacgctgggc gcgactgacg agggatctga
    10021 ggttggcgag gcgccgctca tgatcgtgtc cgaactccgg gaacgccacg cctccgggag
    10081 ccagatcgcg cagcacagcg tggagccaca caccattgac gccgacgcgc gacagacggt
    10141 ccagcaggcc atcaggatag gggtcgggct ccgggtctat cagtgggtcc ccatagactg
    10201 ccacgtacga g
//
LOCUS       DAOSPL010000006         9982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_6752_length_9982_cov_3.697290, whole genome shotgun sequence.
ACCESSION   DAOSPL010000006 DAOSPL010000000
VERSION     DAOSPL010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 9982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9982
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6752_length_9982_cov_3.697290"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3207
                     /locus_tag="PLQ54_00255"
     CDS             <1..3207
                     /locus_tag="PLQ54_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOS91718.1"
                     /translation="PGAAGGLLITGPEFANRDFAGTAEWQQYSFAFRVPEDQPETTLH
                     FGQWHVNGSVEYDDIALYRVMPLHLTEDGIELGAGEQIDGTHFSAVHDLAWDGSTSCR
                     FLEKQGAGFNSNRWLLSAGSEVVYRHQVPGRTFASATANVSIGHYTGGTLVVEASVDG
                     QEWLPAGSANSVTSVDCAIPSRLLPADVVFVRLRFEPETPLQGGLQVYGYRFDAELDT
                     PVTPSRRGATQLWGIIEQTPELPLRLTALSDPSQGELTLGLDTPPGGVPIQVNAELEF
                     LSAPGPGETGASKRTGPATTFGMPLPWAGDYAIELRIASADDGAPLLTARTTVTVADL
                     HDTSYGALLRSDGPIAAWWCDAPHKVSRTRGLPQVRAEAAEIRMARNEYEAVQIVLRP
                     ERDIPEAAVSVTDLTGPAGTIPAADVDLCRVAYLTVEIPSDSSSTPGDWPDPLPPVSG
                     PIALEADRNQPLWVTVHTRADTPPGDYRGALVVEAQGSTLRLPIAVHVYAVTLPTQTH
                     VASSFGLWPGNIFRYHNLTDRDDQRRVWDLYMQNFAAHRIAPIDPMALAPYTLELAGG
                     SPWEGPGRVVAGVDGVPEGERCLHIVDDSATAVAAQDYRPAIPANPGTEYRLEWSVRT
                     GEPGQRYSVTLNTYDQAGQWISGHNIDFTETGSGEWQRVQHLIDDRLDARARFVRIVL
                     RPTPWTEQGEHTGEAWFDDIVFGALNEANAVEDGGFERIYTPDDVEVQLDFTAFDEAA
                     ARYLDELGITAFCLSLHGMGGGSFHSRYLGSLGGYQQGSPEYEQLFSRYLGAVEAHLR
                     EKGWLDKAYTYWFDEPEPRDYDFVKDGMRLLGRAAPSVRRLLTEEVTPELIGSVDIWC
                     PLTPNYDKQSCETRQAAGDEIWWYVCTGPKAPYCGLFIDHSAIEMRMWLWQTWQANVQ
                     GVLVWTTNYWTSDCAYPPPSVQNPWTDPMGWVSGYSTPIGSKLPWGNGDGRFLYPPNT
                     DPEDTTTPYVTGPVNSIRWEMLRDGMEDYEYLWLLRDTIGRARARGADAETLARAEAL
                     LEVSPEVTRSLTEFTKDPAVLLAHRDRVARAIEELSGA"
     gene            3239..4417
                     /locus_tag="PLQ54_00260"
     CDS             3239..4417
                     /locus_tag="PLQ54_00260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS91719.1"
                     /translation="MYRHHVGPARTYYAPAGRADPDELHAARREFISNRLAVTLLEAI
                     PDLALVLNSQRQIIAVNSVLLEAMGVEDPESLVGLRPGELIECVNAEGAPDGCGTSAC
                     CEPCGAVNAILECLATQRACTRECRIRTRRYRDGGAFDLEVRATFVLVGGSGLTIVAM
                     RDISSEKRRDVLERVFFHDVLNTVTGIIAIAELLKTDAEDPAQADELRRDLVQLTEQI
                     TEEIVAQRELLSAERGDLKVQLAPVLADQLLSSVASLYRNHTVARDKTIGLGCCPQIA
                     FETDPALARRVLGNLLKNALEATPRGGAVDLSGEDRGDQVALSVHNAEVMPPEVRSQV
                     FLRSFSTKSPAGRGIGTHSAKLLTERYLGGSISFTSQEPDGTVFTICLPKTRPQPAMP
                     "
     gene            4505..5794
                     /locus_tag="PLQ54_00265"
     CDS             4505..5794
                     /locus_tag="PLQ54_00265"
                     /inference="COORDINATES: protein motif:HMM:NF016439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91720.1"
                     /translation="MLRPEPRVRPETTITTRRRRIPEAVELSEQTEQQFRAAIAEFEN
                     LPLFRALRERGYLAKIGLRGRIPRERYDEYLDVEMVRIIREHGICDVPGWLEELAQPQ
                     AYEQPQRYAAKYRIDAPMVRRVARYCHRLMAQDGATASRGVGAPEVLEVADTAAAPAE
                     SAPEDTTALCAAFVERYGLGPEEFTEGFLSGDADAAALARRFNAPLAEVRRVLQAIDS
                     LRILDVYAGSTQPATTPAEGAPVGEPCAVRVASVEPDPVTGVPRLRIDDDSAYAEAYR
                     LSAQGHQALFELAGDEREAEQLLARLRLINQRKSLLHRVLGVLVARQMRYFVDGDPLS
                     LVPFTQADLARAVGEHQSTISRILQRKLLGFRGEDRPLAWYCQPKRDVVARVVERWPD
                     LSDREVAAVLRDRYDCRVSRRTVNYHRSRTKGTMRNA"
     gene            5787..6215
                     /locus_tag="PLQ54_00270"
     CDS             5787..6215
                     /locus_tag="PLQ54_00270"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOS91721.1"
                     /translation="MRSLSVCRTTLWKCVGALVALASLAGCGGGSIISGTISLAGVTN
                     DLATSSSAGAISGEVLFGDGTFAPGVTVELTADNVDAPVTTTTDRAGLFRFGNVPPAT
                     FTLNLTGSTIEDASDTVIVKAGVITAKRVTVSAAAEAAGG"
     gene            6212..8164
                     /locus_tag="PLQ54_00275"
     CDS             6212..8164
                     /locus_tag="PLQ54_00275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS91722.1"
                     /translation="MKSSHRRGAASAEHLVRQGHEAMTRGDLSVAVERYRAACEADPG
                     WAAAYGGLGAALYRSGQFDEAITVLEEALRLDPTLAESWFTLGLVHKDEQEYEQAEVA
                     FSRAVAAAPEHLQAYYHRGRCRYARGDAGAAAADFRRVVELDPNARDAYYNLAVALAA
                     SRAYDRAEKLIEGEPLLAAHRSDLYVNLGLACSDDPDVPDTAAVRYFEQALEADPGHL
                     EARYRLGLLYARDKYAHPDLRLRAISEFEALLSRPTLHTEFSSPHRVYFAVGTCYDDV
                     DADADLAIDRYQRCLELAPEFAPALNNLAILLQKRGEVRQALDYYSRAILADPDYAPA
                     YHNLSALYYDQDAATMVGHLLDLVQAAQGRGACERVMVQLLPTLVDVARADALASVYD
                     HLHATKNLLGVLGSSIRALAKRAIDGEPPDVVASEVAGLADVQSQAYDGIVEFLRVLR
                     PPPMDTSMVDVNETVRRAVLTVQVQYPQARVELNPSLALPRIRGDARRLREMVVNVVQ
                     NAAQASAGKPVQVSTDLVLGPSGHGSRAEVVRLIVSDSGPGIPESHLRDVLRPGFTTK
                     EGGSGFGLTIAAQIAREHLGRLHVDSTEGEGTTVCIELPAAVELHPEAARLRLRPVIF
                     EDFRGMIRTEVIDAEAADDREPEILQ"
     gene            8161..9636
                     /locus_tag="PLQ54_00280"
     CDS             8161..9636
                     /locus_tag="PLQ54_00280"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOS91723.1"
                     /translation="MSERLVAARAIGDPILVVDDQAAVRDLLTTILSDRGHRVVQAPS
                     GEEALEHLRGQRPFSVVILDLDFGAGRMSGMDVLRRIRADGNDMPVIVLTGQGSVGSA
                     VEAIKCGASDYLEKDAYLEDTIELSMDKIERLVRAIEENRRLAEEYKRLKRQTQYLEE
                     QVGRRYDIVGSSEAIRAALARVERVAKVPRPVLIRGERGTGKELFAAAIHRASPRSEG
                     PFVTVNCAAIPEGLLECELFGQEEDAFNNAPFKLGRFDLAERGTLFLDEIGNMGPEFQ
                     QKILRVIEYRTFERVQGNQTLTADVRIVAATNTDLEAAMAAGTFRADLHDRLAFETIT
                     LPPLRERIDDIPELADHFIQRFRAEVAGLVAHRLSPAALARLADHNWPGNVRELKNVV
                     ERAAYRCDRETIGPEDLDLIPSPRLATEAATATTHAPLLTEGPLPDRIAAFERQAALE
                     ALERCGWNQRKAASRLGLNYDQFRHLYRKYDLGRERPGAQE"
BASE COUNT         1642 a         3613 c         3139 g         1588 t
ORIGIN      
        1 cccggtgccg ccggtgggct tctgatcact gggcccgagt tcgcgaaccg cgacttcgcc
       61 ggcaccgccg agtggcagca gtactccttc gccttccggg tccccgagga ccagcccgag
      121 accaccctcc actttgggca gtggcatgtg aacggaagcg tcgagtacga cgacattgca
      181 ctgtaccgcg tgatgccgct gcatctcact gaggatggca tcgagctcgg cgccggcgag
      241 cagatcgacg gcacacactt ctcggccgtc cacgatctgg catgggatgg ctccacctct
      301 tgccggttcc tggagaagca gggcgcgggc ttcaactcga accgttggct gctctccgcc
      361 ggctccgagg tcgtttatcg gcaccaagtg cctggaagaa cgtttgcctc cgccaccgcc
      421 aacgtgtcga tcggccacta caccgggggg acgctcgtcg tcgaggcatc ggtcgacggg
      481 caggaatggc tccctgccgg ttcggccaac tccgtcacct ccgtcgactg cgccatcccc
      541 tcgcgtctgc tccccgccga tgtcgtcttc gtccggctgc gcttcgaacc tgagacaccc
      601 ctccagggcg gtctgcaggt ctacggatac cgtttcgacg cggagctcga cactccggtc
      661 acgccatccc gccggggcgc cacgcagctg tgggggatca tcgagcagac accggagctg
      721 ccgctgcggc tcaccgccct cagcgatcca agccagggcg agctgactct tggcctcgac
      781 acgcctccgg gcggcgtgcc gatccaggtg aacgccgagc tcgagtttct ctccgcgccg
      841 gggcctggtg aaaccggtgc gagcaaacga acgggtccgg cgaccacgtt cggcatgccg
      901 ctcccctggg ccggcgacta tgccatcgag ctccgtattg cgtccgcgga tgatggcgcg
      961 ccgctgctta cggcgagaac caccgtgacg gttgccgacc tgcacgacac ctcctatggt
     1021 gcccttctgc gctccgatgg ccccatcgcc gcctggtggt gcgacgcgcc gcacaaagtg
     1081 agtcggaccc gcgggctgcc ccaggtccga gcggaagcgg ccgagatccg catggcccgc
     1141 aacgagtatg aggccgtcca gatcgtcctc cgccccgagc gcgatatccc cgaggccgcg
     1201 gtctcggtca ctgacctcac tgggccggca gggaccatac cggcagccga cgtcgacctc
     1261 tgccgcgtgg cctacctcac cgtcgagatt ccgtcggata gctcgtccac cccgggcgac
     1321 tggccggatc cactaccgcc cgtctctggc cccatcgcgc tcgaggccga ccgcaatcag
     1381 ccactctggg tgactgtgca cacgcgggcc gacacgccgc ccggcgacta ccggggcgcg
     1441 ttggtcgtcg aggcgcaggg cagcacgctg cgcctcccca tcgccgttca cgtctacgcg
     1501 gtcacccttc ccactcagac ccacgttgcc tccagcttcg ggctctggcc tggcaatatc
     1561 ttccgctacc ataacctcac cgatcgtgac gaccagcggc gggtctggga tctttacatg
     1621 cagaacttcg ccgctcaccg catcgcaccc atcgacccca tggcgctggc cccgtacacc
     1681 ctcgagttgg ccggtggttc gccctgggag ggccccggtc gcgtcgtcgc cggcgtcgat
     1741 ggggttcccg agggcgagcg gtgcctccac atcgtggatg actcggccac cgccgtggcg
     1801 gcacaggact accgccccgc gatccccgcc aacccgggga ccgagtaccg gctcgagtgg
     1861 tctgtccgga ccggtgagcc cggccaacgc tacagcgtca ccctcaacac ctacgatcag
     1921 gcgggccagt ggatctccgg ccacaacatc gacttcacgg agacaggctc cggcgagtgg
     1981 cagcgcgttc agcatttgat cgacgaccgg ctcgacgctc gggcccgatt cgtgcgcatc
     2041 gttctccgcc ccaccccgtg gaccgaacag ggcgagcaca cgggcgaggc ctggttcgac
     2101 gacatcgttt tcggcgcgct gaacgaggcc aacgccgtcg aggatggcgg cttcgagcgg
     2161 atctacacac ccgatgatgt cgaggtccaa ctggacttca cggccttcga cgaggcggcc
     2221 gcccggtacc ttgacgagtt gggcatcacc gccttctgtc tctcgctcca tgggatgggc
     2281 ggtggcagtt tccactcgcg ctacctcggg agtctcggcg gctaccagca gggctcgcct
     2341 gagtacgaac agctcttctc ccgctacctc ggtgccgtgg aggcccatct ccgcgagaag
     2401 ggctggctcg acaaggcata cacctactgg ttcgatgagc cggagccgcg tgactatgac
     2461 ttcgtcaaag acggcatgag gctactgggc cgggccgccc ccagcgtccg gcgtctcctg
     2521 accgaggagg tcaccccgga gctcatcggc tcggtcgaca tctggtgccc cctaactccc
     2581 aactatgaca agcagtcgtg cgagacccgg caggccgcgg gcgacgagat ctggtggtac
     2641 gtctgtactg gacccaaggc gccctactgc ggcctgttca tcgaccacag cgccatcgag
     2701 atgcgcatgt ggctctggca gacctggcag gcgaacgttc agggggtgct cgtgtggacg
     2761 accaactact ggaccagtga ctgcgcctac ccgccgccat cggttcagaa cccctggacc
     2821 gaccccatgg gttgggtgag cggctatagc actcccatcg gctccaagct tccctggggc
     2881 aatggcgatg gaagattcct ctacccgccc aacacggatc ccgaggacac gacgactccc
     2941 tatgtcaccg gccccgtgaa ctccatccga tgggagatgc tccgcgatgg tatggaggat
     3001 tatgagtacc tctggctcct tcgcgacacc atcgggcgag cccgcgcccg tggcgccgat
     3061 gccgagaccc tcgcgcgggc cgaagcactg ctcgaggtgt cgcctgaggt gacgcgcagc
     3121 ctgacggagt tcacgaagga tcccgccgtt ctactggcgc accgtgatcg cgtggcgcgg
     3181 gccatcgagg agctctcggg cgcctagacc ggccatcggc gacggagggg atgacggcat
     3241 gtaccggcac catgtgggac cggctcgtac gtactacgcg ccggcgggcc gggccgatcc
     3301 ggacgaactc cacgcggcgc ggcgggagtt catctcgaac cggctcgcag tcactctcct
     3361 cgaggcgatc cccgacctcg ccctcgtgct caactcccag aggcagatca tcgcggtcaa
     3421 ctcagtgctc cttgaggcca tgggagtgga ggatcccgag tcgctagtcg gcctgcgccc
     3481 gggcgagctc atcgaatgcg tcaacgccga aggggccccc gatgggtgcg ggaccagcgc
     3541 ctgctgcgag ccgtgtggcg ccgtcaacgc gatcctcgag tgcttggcca cccagcgcgc
     3601 gtgcacgcgt gagtgccgga tccgcacgcg ccggtaccga gatggcggcg ctttcgacct
     3661 cgaagtccgc gccacgttcg tgctcgttgg cggaagcggc ctcaccatcg tagccatgcg
     3721 cgacatcagc tccgagaagc gtcgcgatgt gctcgaacgc gtgttcttcc acgacgtcct
     3781 caacaccgtt acgggcatca tcgccatcgc cgagctgctc aagaccgatg ccgaggatcc
     3841 cgctcaggcc gatgagctca gacgcgactt ggtccagctc acggagcaga tcaccgagga
     3901 gatcgtggct cagcgcgagc tcctctcggc ggagcggggg gacctgaagg tgcagctcgc
     3961 tcccgtactc gcggaccagc tcctgtcttc ggttgccagc ctctatcgga accacacggt
     4021 cgcgcgcgac aagaccatag gcctggggtg ctgcccgcag attgctttcg agaccgatcc
     4081 cgccctggcc cggcgggtcc tcgggaacct gctcaagaac gcgctcgagg ccacgccacg
     4141 cgggggcgcc gttgacctct ccggtgagga ccggggcgac caggtggcgc tgtccgtcca
     4201 caacgccgag gtcatgccgc cggaggtccg gtctcaagtc ttcctccggt cgttctcgac
     4261 caagagtccc gccggccgag gcatcggtac ccacagcgcc aagctactca ccgagcgcta
     4321 cctgggcggg agcatctcgt tcaccagcca ggaacccgac ggcaccgtgt tcaccatctg
     4381 tctccccaag acccgcccac agcctgcaat gccgtaggtc ccccagccct tgtccctctg
     4441 cagttgcctc gccgcgcgcc cgctgctaca ctgccgctgg caaattcgtt gcacggagct
     4501 cgccatgctc agacccgagc ctcgagtccg ccccgagacc accataacca cccgcaggcg
     4561 acgcataccc gaggccgttg agctgtccga gcagaccgaa cagcagttcc gcgccgccat
     4621 tgccgagttc gagaacctgc ctctcttccg tgccctgcgc gagcgagggt acctcgcgaa
     4681 gatcggcctc cgcggccgca ttccgcgcga gcggtacgac gagtacctcg atgtcgagat
     4741 ggtccgcatc atccgcgagc acgggatctg cgacgtgccc gggtggttgg aggagttggc
     4801 gcagccccaa gcctatgagc agccccagcg ctacgccgcc aagtaccgga tcgatgctcc
     4861 catggtgcgc cgtgtcgccc ggtactgcca ccggctgatg gcgcaagacg gtgccaccgc
     4921 ctcacgtggg gtcggcgcgc cagaggtcct ggaggtggcc gacacggcag ccgcaccggc
     4981 cgagtcggcg cccgaggaca ccacggccct atgcgcggca ttcgtcgagc gctacggact
     5041 cggacccgag gagttcaccg agggcttcct cagcggtgac gccgatgccg ctgcgctggc
     5101 ccggaggttc aacgccccgc tggcagaggt ccgacgcgtc ctccaggcca tcgacagcct
     5161 tcgcatcctc gatgtctacg ccggtagcac ccagccggcc acgacgccgg ccgagggcgc
     5221 cccggtcggc gagccctgcg cggtccgcgt ggcatcggtc gaacccgatc cggtcaccgg
     5281 cgtgcctcgt ctgcgcatcg acgacgacag cgcgtatgcc gaggcctacc ggctgagcgc
     5341 gcaaggtcac caggctctct ttgagctggc cggtgacgaa cgcgaagccg agcagttgct
     5401 cgcgcgactc cgtctgatca accaacgcaa gtcgctactt caccgggtct tgggcgtctt
     5461 ggtagctcgt cagatgcgct acttcgtcga tggcgatccg ctctcgctag taccattcac
     5521 gcaggccgac ctcgctcgtg cggtcggcga acaccaatcg accatctcgc gcatcctgca
     5581 gcgcaagcta ctggggttcc ggggtgagga tcggccgctg gcatggtact gccagccgaa
     5641 gcgcgatgtc gtggctcgag tggtcgagcg ttggcccgac ctctcggatc gtgaggtggc
     5701 ggctgtgctc cgtgaccgct acgactgccg ggtgagccgg cgaaccgtca actaccaccg
     5761 gtctcggacc aaggggacga tgcggaatgc gtagtctgtc cgtgtgtcgc acgacattgt
     5821 ggaaatgcgt gggggcgctg gttgctctcg cctcgctcgc cggttgtgga ggcggttcca
     5881 tcatctcggg aaccatcagc ctcgccggtg tgaccaacga cctggccacc tcatcctcgg
     5941 cgggcgccat cagtggcgag gttctgttcg gcgacggcac gttcgccccc ggcgtgacgg
     6001 tcgagctcac cgccgacaac gtcgacgccc cggtcacgac gaccacggac cgagccgggc
     6061 tgttccggtt cggcaacgtt cctccggcca cgttcacgct gaacctcacc ggctcgacga
     6121 tcgaggacgc gtccgacacg gtcatcgtga aagccggtgt gatcaccgcc aagagagtga
     6181 cggtgagcgc ggccgcggag gccgcgggcg gatgaagtcg agccaccggc gcggtgcagc
     6241 cagcgcggaa cacctcgttc gccagggcca cgaggccatg acccggggcg acctctccgt
     6301 cgccgtcgaa cgataccggg cggcctgcga ggccgacccg ggctgggccg ctgcctatgg
     6361 aggcctcgga gccgccctct atcgaagcgg ccagttcgat gaggccatca ccgtgcttga
     6421 ggaagccctt cgcctcgacc cgaccctggc cgaatcatgg ttcaccttgg gcctcgtcca
     6481 caaggacgag caggagtacg aacaagccga agtggccttc tcacgtgccg tcgcggccgc
     6541 tcccgagcac ttgcaggcct actaccaccg cgggcggtgc cgctacgcgc ggggggatgc
     6601 gggcgccgcc gccgccgatt tccgccgcgt tgtcgaactc gaccccaacg cccgcgacgc
     6661 ctactacaac ctggccgtcg ctctggccgc cagccgcgcc tatgaccggg ccgagaagct
     6721 catcgagggc gagcccctac tcgccgccca ccggtccgac ctctatgtga accttggcct
     6781 cgcctgcagc gacgaccccg acgtgcccga cacggctgcc gtccgctact tcgagcaagc
     6841 actcgaggcc gatcccggcc atctcgaagc ccgataccgg ctgggcttgc tctacgctcg
     6901 cgacaagtat gctcatcccg acctgcgcct acgagccatc agcgagttcg aggccctgct
     6961 gtcccgcccc accctgcaca ccgagttctc cagcccccat cgcgtctact tcgccgtcgg
     7021 cacctgctac gacgatgtcg acgccgacgc tgacctggcc atcgaccgct accagcgctg
     7081 tctcgaactc gccccggagt tcgcgcccgc cctgaacaac ctcgccatcc tcctccagaa
     7141 gcgcggcgag gtgcgccagg ccctggacta ctacagccgc gccatcctcg ccgaccccga
     7201 ctacgcaccg gcctaccaca acctgtcggc gctctactac gaccaggatg ccgcgaccat
     7261 ggtggggcac ctgcttgacc tggtgcaggc cgcccagggc aggggagcct gcgaacgcgt
     7321 gatggtccag ctcctgccga ccctggtcga tgtggcccgg gctgatgcgc tggccagtgt
     7381 ctacgaccat ctccacgcca ccaagaacct tctgggtgta ctgggtagct cgatccgggc
     7441 gctggccaag cgggccatcg atggcgagcc gcctgacgtc gttgccagtg aggtcgctgg
     7501 cctggccgac gtccagtcgc aagcctacga cggtatcgtg gagttcctcc gtgtgctccg
     7561 gccgcccccc atggacacga gcatggtcga tgtcaacgag acggtgcgcc gtgccgtcct
     7621 cacagtgcag gtgcagtatc cccaggcccg cgtcgagctg aacccgtccc tggcgctgcc
     7681 acggatccgc ggcgacgccc gccggctgcg tgaaatggtc gtcaacgtgg tccagaacgc
     7741 cgcccaggca tcggccggaa agccggtgca ggtgtccacc gatctagtcc tcggcccctc
     7801 cggacatgga tcgcgcgccg aagtggtgcg cctcatcgtg tcggattccg gccccgggat
     7861 ccccgagtcg cacctgcgcg atgtgctccg gcctggcttc accaccaaag agggaggcag
     7921 cgggttcgga ctcaccatcg ccgcccagat cgcccgcgaa cacctcggga ggctccatgt
     7981 ggatagcacc gagggagaag gcacgaccgt ctgcatcgag ctgcccgcgg ccgtcgaact
     8041 ccaccccgaa gccgcccgct tgcgcctgcg tcccgtcatc ttcgaagact tccgcggcat
     8101 gatccgtacc gaagtcatcg atgccgaggc cgccgatgac cgcgaaccgg agatcctcca
     8161 atgagcgaaa gactcgtcgc agcccgcgcg ataggcgacc ccattctcgt cgtcgacgac
     8221 caggcggccg tgcgcgacct gctaactacg atcctgtccg atcgtggcca tcgtgtcgtc
     8281 caggccccct cgggtgagga ggccctggaa cacctccgcg gccaacgccc cttctctgtc
     8341 gtcatcctcg acctcgactt cggcgccggg cgcatgagcg gcatggatgt cctccggcgg
     8401 atccgcgccg acggcaacga tatgccggtt atcgtgctga cgggccaggg cagcgttggc
     8461 tcagccgtcg aggccatcaa gtgcggcgcc tccgactacc tcgagaaaga tgcctacctc
     8521 gaggacacca tcgagctgag catggacaag atcgagcggc tggtgcgtgc catcgaggag
     8581 aaccgccggc tcgccgagga gtacaagcgc ctcaagcgcc agacacagta cctcgaagag
     8641 caggtcggcc gacggtacga catcgtgggg tccagcgagg ccatccgcgc cgcgctggcc
     8701 cgcgtcgagc gcgtcgccaa ggtcccgagg cccgtgctca tccgcggcga aaggggaacc
     8761 ggtaaggagc tcttcgccgc cgccattcac cgcgccagcc cgcgcagcga gggacccttc
     8821 gtcaccgtca actgcgccgc catccccgag ggcctcctcg aatgcgagct gttcggccag
     8881 gaggaggatg ccttcaacaa cgcgcccttc aaactcggcc gcttcgacct cgccgaacgg
     8941 ggtaccttgt tcctcgacga gatcggcaac atgggccccg agttccaaca gaagatccta
     9001 cgagtcatcg agtaccgcac cttcgagcgc gtccagggca accagacact caccgccgat
     9061 gtccgcatcg tcgcggccac caacacggat ctggaggccg ccatggccgc gggcaccttc
     9121 cgcgcggatc tccacgaccg gctggccttc gagaccatca ccttgccgcc gctccgtgag
     9181 cgaatcgatg acatccccga actggcggac cacttcatcc agcggtttcg cgccgaggtc
     9241 gccggactcg tggcccaccg gctctccccg gccgccctgg cgcgcctggc cgaccacaac
     9301 tggccgggga acgtccgcga gctcaagaat gtggtcgagc gggctgccta ccgctgcgat
     9361 cgtgaaacca tcgggcccga ggacctcgac ctgattccat cgccgcggct ggccaccgag
     9421 gcggccaccg ccaccacaca cgcgccgctc ctgaccgagg gaccgctgcc cgaccgcatc
     9481 gccgcattcg agcgccaggc ggctttggag gcactcgaac gctgcgggtg gaaccagcgt
     9541 aaggccgcca gccgcctcgg tctgaactac gaccagttcc ggcacctgta ccgcaagtac
     9601 gatctcggcc gagagcgacc gggggcacag gaatagcgag gtccggcgcc catggggccc
     9661 gccccggcgg aggcgtcact ccgaaccgca tagcgacggg ggaccttggc gcctggtagg
     9721 ggcgtgcacg gcgttccacc acacgccgag atggctcacc atgctcggga tgacgtcgtc
     9781 gccggtccgt gcgcttcgct ctcgccacgt cgcgaacctg gaggtcgatc gatgaaggtc
     9841 tacctgatga ccgactacga gggtgttgca ggcgtctgga actgggagcc ccgtgacgac
     9901 gtcagccccg ccgcctgccc gcgcaggggc atgaacacca gcagcgtgca ggcatcgccc
     9961 gcgtcctgcg gccggaagcg ga
//
LOCUS       DAOSPL010000007         9872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_6844_length_9872_cov_3.384435, whole genome shotgun sequence.
ACCESSION   DAOSPL010000007 DAOSPL010000000
VERSION     DAOSPL010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9872
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6844_length_9872_cov_3.384435"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3217)
                     /locus_tag="PLQ54_00285"
     CDS             complement(<1..3217)
                     /locus_tag="PLQ54_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91724.1"
                     /translation="MMTAALVTLSTFIAQAAMADMPAADMIWDGVRAYDTATVANSPS
                     STVQAACAAGQVTRRAIGLHPGAGLSTVTYPPLEVEVGAGQRALLVGYAGISDGVPWD
                     DAKHRPDGVTFHVIVDGEELFVTHLAESVWRPFAIPLGPGADGVLRCHITLATDAGPA
                     GNSSYDWAFFGEPAVVAVSGSPIEPRTAVGGLSGILLAHITAGEGTLAVEGIDEASQP
                     VPGARVEERVPPGTEHAFLQFDFAQIPECAGWRWVTEGPTCDAAHGGCWAPSVRVAGA
                     GPAQAITLEGEPLYWRVTLVNDGRGVLLPEHRATIRCGAEERAAGRMAPGDTQVFEFD
                     LGPRSPGDTEIVEVSAELPAAHGERLDVGFARNAVIWPALPPLPNGRPGDAHSVELAG
                     RYTLLENRDSRWLFGPGFVGALVYAWGVDGWEPVGSLGPLAAFQLPDGSHMPDFDVAE
                     AARTPDGARLLLTRRAKGFLCRVSLELPDSGPALSIDMHFSAEQNAQTCGIWGPEVRP
                     GDRVGGSDKGVALFPGLEYLEGAEASSSTRDLAPPHANRTVPPKFHVTLGLMAVEIRP
                     GGPLMGIAWDPRQRWDGEHMAPAAVFSSPDQLDYQDNHLMHLALPSVPAMMPRNERRL
                     AEPMRVPEGTELSLSQRVFAGRFEPDASAVLAWYDSAIGYPEAEGWPRSFEDEMALSR
                     HAFLVTVWDEATRKSRHVVGWDAANSPGFATLLLTDGRAVTQGEAARQVLDRAELIGR
                     QTLEQDGPSGLTSSASCHIMGGEFPYHWGQLPAALVGLRNAARAAMARQEADGGWGFY
                     PDEKHATLGEPGTKTAGIAAPQALQIAKWAAISGDPECEAALRRAVDFLRAQAVPRGA
                     QGWECPILQPDVLASAYAVRACVWAYMALGDPDYIEEAQRWARTGLAFQYAWDDGAHP
                     GMRYASIPVFGSTFHTHSWIGLPVQWCGLVYAYSLQELCRIAPDDLWRRQIEGITVSA
                     MHQQWPMDNAELAGTYPDSYGKDFTVRNGVFINPEGIVLNLLAMRGFDPGLRSRAIDL
                     DGARLHMTAAAGIDAKPDDQGVRVTLSRVIGPVAYATIGPVT"
     gene            3451..5184
                     /locus_tag="PLQ54_00290"
     CDS             3451..5184
                     /locus_tag="PLQ54_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOS91725.1"
                     /translation="MNGCQRPTPRWLAVLVVGAVAAAAVQAWAAPKRQPLPPAQVRAV
                     GDSLSRHLLGSRRLDRAGHLVLTFYVLGDSRAGGERDLLVTKVTRDVFDRKILGEIME
                     RIIKDIEADRPAAPDAPCFVFHTGDITRRPDDEEDWERVRNAIAPLVTRDDLNVSVLP
                     VIGNHEYHSTDGSTPDQEELKYYYATFGGEIDGHPVIKDPALRAQFNSEAPPRWYRVH
                     CRNTDFIVLDSCERSFLTPGSPATGLLPEQVYDLRRWLLEPPEGPVTNVFLMQHHPIF
                     SNGQGHPDLEPGSSRDAATGKTYRTTEMAQWIVRRRLLKQVLDEYYLARASEGSRVRA
                     TFHAHNHTYERIIIPPAYLPGWDKVEDLGAQYLTGPEGQSYADYVREVLKLPEAISQP
                     VNANGAFLDRDIRFMTSGGAGAPHRKKGGLFGGKDKTHVPLSREQRDQAYAKGQLERW
                     VPDFYRYRYVRNPRDEYPEVANMTRVLDGDFVPIARVGWVRRLQQLTNADVQGAEGAL
                     GIMPPAKWWELFSKKIPSDSEQYHFIKVRVYEGAIAFDMYLKYAGAWLHADHDEVTTG
                     SPGQRVFVPGM"
     gene            5301..6728
                     /locus_tag="PLQ54_00295"
     CDS             5301..6728
                     /locus_tag="PLQ54_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOS91726.1"
                     /translation="MSCGSEYDSWHRGGLLGPNGREVFRAIAAAGEYLMSRTPSELLE
                     HVGRTVDAVPAVTHLDKNVIQASYGGRRESIPNDMLSGRGLFFITEQRRLMLDCTAGH
                     YQMTWGYRHPVLDAAVREALDAGIVPDDHSNIPGAPVKRLAYKLAELCNPDDAGLPQC
                     DDSGVRDNDASLNTVLLGIVTGSVACSTALKVMLARHRAQKGTRPAIVTLEGNYHGTD
                     MFAQRMRHMWADYFTDFDFVNVEPNDLAGLEDAFDGLGERVMGVMLEPVMMNREAILV
                     EPDFAQRIRELCDRVDALMAVDEIQTGFWCPEVFHYKRWGVVPDFVICGKGMTAGVHP
                     LSAVIFRRPLDILAQYDSISTNGGAPLAAHAALHSIALIEAEAERIDRVAQGMEAMMR
                     GLVAQFPDVLKSYQGDGFLSGLKFHRVEHALEFHRRCIERGLWVRVHAYHEGHSTILT
                     KFALLLDDPVIEFVRQAFAELLRGL"
     gene            6746..8242
                     /locus_tag="PLQ54_00300"
     CDS             6746..8242
                     /locus_tag="PLQ54_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOS91727.1"
                     /translation="MREVALGIDLGTTAVKVGAFDVVSGRPVAVSAQPHELMTPQPDW
                     VELEVETYWDCTVRGVRDVMRQAGRDTQVVSIGLASQGQTFVVLDAQGRPLRPAIVWI
                     DTRAREELEEFARAVDYDRFYRNTGVPFPNVIDSAPKILWVKRHEPDVFAAARYIIVL
                     PAYIALRLTGEPVADPGNAGSTGLLDEGTLGWWPEAVAAVGVDAALLGRVAPSGSMIG
                     GLTSAAAQELGLPEGIPVGTGANDQSAAAVAMGNRHPGQLSATIGTAMAVVGTVAPTD
                     DAYSAGLLVGRHPLGDALSILAYAKTAAILLTWFRKAFGVKESYEDLTGEAARAPVGC
                     DGLTCIPHLTGMGTPSFDDGVRGAFLHVGLQHERRHFLRAMVEAVCFGARDSVDQMRA
                     VGAAFGDLIVSGGATQSPTWMQALADCLRLPVRVPECAEAACRGGALLGLIAAGRRAE
                     ALAWPASEAGREQRYEPDADQAPAYDDAYGRYVRAMDTLYPGARRAGV"
     gene            8264..9301
                     /locus_tag="PLQ54_00305"
     CDS             8264..9301
                     /locus_tag="PLQ54_00305"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOS91728.1"
                     /translation="METASLWITEPEYFVECDLPIYHDELEGFLPERILDCHCHTYLL
                     EHLARELTPEEKSKSYGVEEKPFSIEDVRACFAALFPGKRCEALVFGTPSAYFSLEAQ
                     NAYVEDQLREPGIDGLALLSPRAREDDLEGLADAGFAGFKPYDQLVGKPLPEVTIRDM
                     VPEAARRVADRRRLIIMLHVPRVGRIADPDNIREVCELCDECPGAQVILAHIGRAYGP
                     WYIEQAIGELSRRPNLWYDTAALDDADSIDTVLRHCGPGKLLFGTDLPIAAMRGKHLC
                     VNRQCLFLTRDRFPWSVSSEKPGELKLTFFAYETVRALRRACERHGLSRAEVEAICYD
                     NARQMIDRAMA"
     gene            9390..>9872
                     /locus_tag="PLQ54_00310"
     CDS             9390..>9872
                     /locus_tag="PLQ54_00310"
                     /inference="COORDINATES: protein motif:HMM:NF014649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91729.1"
                     /translation="MRPRVGLLGLTLDLYARTSPEMLEGLRAFSGELRETLRAVADVD
                     EAELAWDRRAIEKAVARFDRDDVDLIIIVLLSYSTSLSAMPALLRTRKPLLIWNTQKL
                     RQVGPDFGPDDITYNHGMHGVQDLCNVLLRHGREFHLITGHYQDAAAVGKVHDLIRAA
                     A"
BASE COUNT         1587 a         3100 c         3577 g         1608 t
ORIGIN      
        1 cggtcacggg cccaatggtc gcataggcca ccggtccgat gacgcgcgag agcgtgacgc
       61 gcacgccctg gtcatcgggc ttggcgtcga taccggctgc ggcggtcatg tggaggcgcg
      121 cgccatccag gtcgatcgcc cgcgaacgca ggccggggtc gaagccgcgc atggccagca
      181 ggttcagcac aatgccctcg gggttgatga agacgccgtt gcgaacggtg aagtccttgc
      241 catagctgtc cgggtacgtg ccggccagct cggcgttgtc catgggccac tgctggtgca
      301 tggcgctgac ggtgatgccc tcgatctggc ggcgccagag gtcatcggga gcgatgcggc
      361 acagctcctg gaggctgtag gcgtagacca ggccgcacca ctgcacgggc aggccgatcc
      421 atgagtgggt gtggaaggtg ctgccgaaga cggggatgct cgcgtagcgc atgcccgggt
      481 gggcaccgtc atcccaggca tactggaagg ccagaccggt tcgagcccag cgctgtgcct
      541 cctcgatgta gtccgggtcg cccagggcca tgtaggccca gacacaggct cgaacggcat
      601 aggccgacgc caggacgtcg ggctgaagga tggggcactc ccacccctgg gcgccgcgtg
      661 ggaccgcctg ggcgcgcagg aagtccacgg ctctgcggag ggcggcctcg cactcggggt
      721 cgccgcttat ggcggcccac ttggcgatct gcagagcctg gggcgcggcg atgcccgcgg
      781 tcttggtgcc gggttcgccg agggtcgcgt gtttctcgtc ggggtagaag ccccagccgc
      841 catcggcctc ctgacgcgcc atggctgctc gggccgcgtt gcgcagcccg accagagcgg
      901 cggggagctg gccccagtga taggggaact cgccgcccat gatgtggcag ctcgcggagc
      961 tggtcagtcc gctgggaccg tcctgttcga gtgtctgacg tccgatcaac tcggcgcggt
     1021 cgagcacctg cctggcggcc tcgccctgtg tgacggcgcg gccatcggtg agtaggaggg
     1081 tggcgaagcc cggtgagttg gcggcatccc agccgaccac atggcggctc ttcctggtgg
     1141 cctcatccca caccgtgacg aggaaggcgt ggcgcgacag ggccatctcg tcctcgaagc
     1201 tccggggcca gccttcggct tcggggtacc cgatggccga gtcgtaccag gcgagcacgg
     1261 ccgatgcgtc gggttcgaac cgtccagcaa agacgcgctg gctgagggac agctcggtac
     1321 cctcgggtac gcgcatcggc tcagccagac gccgctcgtt tcgcggcatc atggcgggca
     1381 ccgagggtag cgcgaggtgc atgaggtggt tgtcctggta gtcgagctga tcgggcgatg
     1441 agaacacggc ggccggcgcc atgtgctcgc catcccacct ctgccgggga tcccaggcta
     1501 tacccatcag agggccgccg gggcggatct cgacggccat gagtccaagg gtgacgtgga
     1561 acttcggtgg gaccgtgcgg ttggcgtggg gcggcgcgag gtcgcgggtg ctcgagctcg
     1621 cttcggcccc ttcgaggtac tcgagaccgg ggaagagggc cacgcccttg tcggagccgc
     1681 cgactcggtc gcccggccgc acctcgggtc cccagatgcc acacgtctgc gcgttctgct
     1741 cggcgctgaa gtgcatgtcg atggacaggg caggccccga gtcgggtagt tcgagcgaca
     1801 cgcggcagag aaacccctta gcccggcggg tcagcagcag ccgagcgccg tcgggggttc
     1861 gcgcggcctc ggccacgtcg aagtccggca tgtgggaacc gtccggcagc tggaaggcgg
     1921 ccagtgggcc gagcgatccg accggttccc agccgtccac gccccaagcg tataccagag
     1981 cgccgacgaa cccgggaccg aacagccagc gggagtccct gttctcgagc agtgtgtatc
     2041 gtccggccag ctcgacggag tgggcgtcgc ccggccgccc attgggcagg gggggcagcg
     2101 cgggccagat gacggcgttg cgcgcgaaac ccacgtcgag ccgttcgcca tgcgcagcgg
     2161 ggagctcggc gctgacctct acgatctcgg tgtcgcccgg actcctcggg cccaggtcga
     2221 actcgaacac ctgcgtgtct ccgggcgcca tccggccggc ggcgcgctct tcggcgccgc
     2281 accggatggt ggcccggtgc tcgggaagca ggacaccgcg gccgtcattc acgagcgtga
     2341 cacgccagta gaggggttcg ccctcgagcg tgatcgcctg ggcgggacca gcacccgcga
     2401 ctcggaccga gggcgcccag cagccgccat gggccgcatc gcaggttggt ccctcggtca
     2461 cccagcgcca cccagcgcac tcggggatct gggcgaagtc gaactgcaag aaggcgtgct
     2521 cagtgcccgg cgggactcgt tcctcaacgc gagcgccggg aacgggctgg ctggcctcgt
     2581 cgatgccctc gacagccaga gtgccttcgc cggcggtgat gtgagccagc aggatgcctg
     2641 agagcccgcc gacggcggtt cggggctcga tgggcgatcc gctgaccgcc acgacagccg
     2701 gctcgccgaa gaacgcccag tcgtagctgc tgttgccggc agggcctgcg tcggtggcca
     2761 gagtgatgtg gcagcgaagg accccatcgg cgccgggacc taaagggatg gcgaagggac
     2821 gccagaccga ctcggccaga tgggtgacaa acagctcctc gccatcgaca atgacgtgga
     2881 aggtcacccc gtcgggccgg tgtttggcat catcccaagg gacgccgtcg gagatgccag
     2941 cgtagccgac cagaagcgcg cgctggcccg cgccgacctc gacctcgagc ggaggatagg
     3001 tcacggtcga caagccggcg ccagggtgca ggccgatggc acgccgcgtc acctgtccgg
     3061 cagcgcaagc ggcctgcacg gtcgacgacg gcgagttggc gaccgtcgcg gtgtcatacg
     3121 cgcggacgcc gtcccagatc atatcagcgg cgggcatgtc ggccatcgct gcctgagcaa
     3181 tgaaggtgga cagcgtgacc agcgcggcgg tcatcatgcg tcgcacactc cttcgcgtga
     3241 ggcgaggttg tggttcgggc gattcgccgc gaggcggcgg gaagcctgtc ggcgaacgag
     3301 accgcggaac tgcggcgctc gaaggccgcc tgcgccgcga acccgtgtgg ggaacgagcc
     3361 acggtggcgc ggggttgcgc cagttggggc tgacatatcc ttgggccgga cccgccccat
     3421 ggctctgggg acgtacggag gttgtgcgcg atgaacggat gccagcggcc gacaccgcgc
     3481 tggttagcgg tcctcgtcgt gggcgccgtc gccgcggccg cggtgcaggc atgggccgcg
     3541 ccgaagaggc agccgctccc accggcacag gtgagggcgg tcggtgacag cctgagtcgg
     3601 cacctgttgg gttcgcgcag actcgatcgt gcgggtcatc tggtgctgac gttctacgtt
     3661 ctgggtgatt cgcgggcggg tggtgagcga gacctgctgg tcacaaaggt gacccgtgat
     3721 gtgtttgacc gcaagatcct gggcgagatc atggaacgca tcatcaagga catcgaggcg
     3781 gaccgcccag ccgcacccga cgctccctgc ttcgtgtttc acacgggaga catcacacgc
     3841 cggccggatg acgaggagga ctgggagcgc gtacgcaacg ccatcgcgcc gctggtaacg
     3901 cgggacgatc tgaacgtgag cgtcctgccg gtcatcggca atcacgagta ccacagcacc
     3961 gacggcagta cacccgacca ggaggagctg aagtactact acgcgacttt tgggggagag
     4021 attgacgggc atccggtgat caaggatccg gcgcttcgag cccagttcaa tagcgaggcg
     4081 cctccgcgat ggtaccgcgt gcactgccgg aacacagact tcatcgtgct ggactcgtgc
     4141 gagcggtcat tcctgacgcc cggctccccg gcgaccggct tgctccccga gcaggtgtac
     4201 gacttgcgcc gatggctgct ggagccgccg gagggtccgg tcacgaacgt tttcctgatg
     4261 cagcaccacc cgatcttctc gaacgggcag ggccatccgg atctcgaacc agggtcgagc
     4321 cgcgacgcgg ccaccggcaa gacgtaccgc acgaccgaga tggcgcagtg gattgtgcgg
     4381 cgcaggttgc tcaaacaggt gctggacgag tactacctgg ctcgggccag tgagggctcg
     4441 cgcgtccgcg ccacgttcca cgcccacaat cacacttacg aacgcatcat catcccgccg
     4501 gcctaccttc ccggctggga caaggtcgag gacctggggg cgcagtacct caccggtcct
     4561 gagggtcaga gctatgccga ctatgtgcgt gaggtgctga agctgcccga ggcgatcagt
     4621 cagccggtga acgcgaacgg ggcattcctg gaccgggaca tccgtttcat gacctccggc
     4681 ggggccgggg ctccccaccg caagaagggg ggtctcttcg gcggcaagga taagacacat
     4741 gttccgctct cccgcgagca gcgagaccag gcgtatgcca agggacaact ggagcgctgg
     4801 gtgccggact tctaccgcta ccggtacgtg cggaacccga gggatgagta cccggaggtc
     4861 gccaacatga ctcgggtgct cgatggtgac ttcgtgccga ttgcgcgcgt ggggtgggtg
     4921 cgccgcctgc agcaactgac gaatgcggac gtccagggcg ccgagggagc cctggggatt
     4981 atgccgccgg ccaagtggtg ggagctcttc tcgaagaaga tccccagcga cagcgagcag
     5041 tatcacttca tcaaggtccg cgtatatgag ggcgccatcg cgttcgacat gtacctcaag
     5101 tatgccggcg catggctaca tgccgaccac gatgaggtga ccacaggctc tccgggccag
     5161 cgcgtgttcg tgccgggcat gtaggctcga gtgcccctgt gggcacatgc ccgccggacg
     5221 ctgggccggt gcaggtgcta gactgtcacg accgggtagc gacgcggcag gtgttggtcg
     5281 tgtcggagcg gaggcgtgag atgagctgcg gcagcgagta cgattcttgg caccggggtg
     5341 gactgctggg ccccaacggt cgggaggtgt tccgggccat cgcggcggct ggcgagtacc
     5401 tgatgtcgcg cacaccgtcg gagctgctgg agcacgtggg ccgcaccgtc gacgcggtgc
     5461 cggcggtgac ccacctggac aagaatgtga tccaggcgtc gtacggcggc cggcgcgaat
     5521 cgatcccgaa cgatatgctg agtggtcgtg gcctgttctt catcacagag cagcgccgac
     5581 tgatgctcga ctgcacggcg ggccactacc agatgacgtg gggctatcgc caccctgttc
     5641 tggacgcggc ggtacgcgag gcgctcgatg cggggatcgt gcccgacgac cacagcaaca
     5701 tccccggcgc gccggtgaag cggctggcct acaagctggc cgagctgtgt aacccggacg
     5761 acgcgggcct gccgcagtgc gatgactcgg gagtccgcga caacgacgcg tcgctgaaca
     5821 cggtgctcct gggcatcgtg acgggctccg tcgcctgctc gacggcgctg aaggtcatgt
     5881 tggcccggca tcgcgcgcag aagggcacgc gtccggccat agtgacgctg gagggaaact
     5941 accacgggac cgacatgttc gctcagcgga tgcggcacat gtgggcggac tacttcaccg
     6001 acttcgactt cgtgaatgtc gagccgaacg acttggctgg cctcgaagac gcgttcgatg
     6061 gcttgggcga gcgcgtcatg ggcgtgatgc tcgagccggt gatgatgaac cgcgaggcca
     6121 tcctggtcga gcccgacttc gctcagcgga tccgtgagct gtgtgaccgg gtcgatgcgc
     6181 tgatggccgt cgatgagatt cagacgggct tctggtgtcc tgaggtattc cactacaagc
     6241 gctggggcgt ggtgcccgac tttgtcatct gcggcaaggg gatgactgcc ggggtgcatc
     6301 cactctcggc ggtgatcttc cgccgtccgc tggacattct ggcccagtat gacagcatca
     6361 gcacgaatgg cggggcgccg ctggccgccc acgcggccct ccattcgatc gccctgatcg
     6421 aggccgaggc cgagcgcatc gaccgagtcg cccagggcat ggaggccatg atgcgtggac
     6481 tggtggcgca gtttccggac gtgctgaaga gctaccaggg ggatggcttc ctcagtggtc
     6541 tcaagttcca ccgtgtcgag cacgcgctcg agttccatcg ccgttgcatc gagcgcgggc
     6601 tctgggtgcg cgtgcacgcc taccacgagg ggcacagcac gatcctgacg aagttcgcgc
     6661 tcctgctgga tgacccggtg atcgagttcg tgcggcaggc gttcgcggag cttctgcgag
     6721 ggctataggg gggcgtcagg ccgacatgcg tgaggtggcc ctgggcatcg acctgggcac
     6781 gacggcggtg aaagtcggcg ccttcgacgt ggtatccggc cggcccgtcg ccgtgtccgc
     6841 gcagcctcat gagctcatga cgccacagcc cgactgggtc gagctggagg tagagaccta
     6901 ctgggactgc acggttcgtg gtgtgcgtga tgtgatgcgg caggccgggc gcgacactca
     6961 agtggtgtcg attggcctcg cctcccaggg tcagaccttc gtggtgctcg atgctcaggg
     7021 gcgccccctg cgtcccgcca tcgtgtggat cgacacccgg gcacgagagg agctcgagga
     7081 gttcgccagg gccgtcgact atgaccgttt ctaccgcaac acgggcgtgc cgttccccaa
     7141 tgtgatcgac tcggccccga agatcctctg ggtgaagcgc catgagcccg atgtgttcgc
     7201 tgcggcccgg tacatcatcg tgctgccggc ctacatcgcg ctgcggttga cgggggagcc
     7261 ggtggccgat cccggcaatg cgggcagcac ggggctcctc gacgagggca cgctggggtg
     7321 gtggcccgag gcggtagcag cggtgggagt tgatgcggcg ctgctcgggc gtgtcgctcc
     7381 atcaggcagc atgatcggcg gcctgacgtc cgcagcggct caagagctgg ggctgccgga
     7441 gggcatccct gtcggcacgg gggccaatga ccagtcggcc gcagctgtgg ctatggggaa
     7501 ccgccatccc ggtcagctct cggcgacgat cgggaccgcg atggcggtgg tcgggaccgt
     7561 ggcgcccact gacgacgcgt actcggcggg gctcctggtg ggccggcatc cgctgggcga
     7621 cgcgctgtcg attctggcgt atgcgaagac ggccgcaatc ctgctgacat ggttccggaa
     7681 ggccttcggc gtcaaggaat cgtacgagga cctgaccgga gaggccgcgc gcgccccggt
     7741 ggggtgcgat gggctgacgt gcatcccgca cctgacaggc atgggcacgc cttcgttcga
     7801 tgacggtgtg cgtggcgcgt tcctgcacgt gggtctgcag cacgagcgcc ggcatttcct
     7861 tcgcgccatg gtggaggccg tctgttttgg tgcgcgagac agtgtcgacc agatgcgtgc
     7921 ggttggagcg gcctttggtg acctgatcgt gagtggcggg gcgacgcagt cgccgacgtg
     7981 gatgcaggcc ttggccgact gcctgcggtt gccggtccgg gtgccggagt gcgcggaagc
     8041 ggcgtgccgg ggcggcgcgc tgttgggcct catcgccgcg ggccgccgcg ccgaggctct
     8101 cgcctggccg gcttccgaag cgggacgtga acagcggtac gaaccggacg cggatcaggc
     8161 gccggcgtac gatgatgcct atgggcgcta cgtgcgcgcc atggacaccc tgtaccctgg
     8221 tgcgcgtcga gccggggtat gacggcacgg aggcggggcg cccttggaga cggcatcctt
     8281 gtggatcacc gaaccggagt acttcgtcga atgtgatctc ccgatctacc atgacgagct
     8341 cgaggggttc ctgcccgagc gcatcctcga ttgccactgc cacacgtacc tgctcgagca
     8401 cctggcgcgg gagctgacgc ccgaggagaa gtccaagtcc tacggcgtcg aggagaagcc
     8461 cttctcgatc gaggatgtgc gggcgtgctt cgcggccctg ttcccgggga agcgatgtga
     8521 ggcgctggtg tttgggacgc cgagcgccta cttcagcctg gaggcgcaga acgcgtacgt
     8581 ggaggaccag ctgagagaac ccgggatcga tgggctcgcc ctgctgtcgc cccgggcgcg
     8641 cgaggacgat ctggaagggt tggccgatgc cgggttcgcg gggttcaagc cctatgacca
     8701 gctcgtgggg aagccgctac ccgaagtgac gattcgtgac atggtgccag aggccgctcg
     8761 gcgcgttgcc gaccggcgac ggctgatcat catgctgcac gtgccccgcg tgggccggat
     8821 cgccgatccg gacaacatcc gcgaggtgtg tgagctctgt gatgagtgcc cgggcgcgca
     8881 ggtcatcctg gcgcacatcg gtcgggccta tgggccctgg tacatcgagc aagccatcgg
     8941 cgagctgagc cggcgcccca acctgtggta tgacaccgcg gcactggacg acgccgactc
     9001 gatcgacacg gtcttgaggc attgcggacc tgggaagctg ctgtttggca ccgacctgcc
     9061 catcgcggcg atgcgaggca agcacctgtg cgtcaaccgt cagtgcctct tcctgacgcg
     9121 cgaccggttc ccgtggtcgg tgtcttcgga gaagccgggg gagctgaagc tgacgttctt
     9181 cgcgtacgag acggtccggg cgctgcgacg tgcctgcgag cgccatgggc tctcccgggc
     9241 cgaggtggag gccatctgtt acgataatgc gcgccagatg atcgaccgcg ccatggcgtg
     9301 aagcggcgga cgacggagac ccggcgggga gctccaccac tctgatccgc tcccgatggg
     9361 agtggaagcc gattcggagg catggcccca tgcgacctcg agtaggcctg ctgggtctga
     9421 cactggacct gtacgctcgg acttcgcccg agatgctcga gggcttgagg gctttctccg
     9481 gcgagcttcg cgagacgctc agggctgtgg ccgacgtgga tgaggccgag ctggcctggg
     9541 accggcgggc cattgagaag gctgtggccc ggtttgaccg cgacgacgtg gacctcatca
     9601 tcatcgtact gctgtcgtac tcgacgagcc tttcggcaat gccggcgctg ttgcggacgc
     9661 gcaagccgct gctgatctgg aacacacaga agctgcgcca ggtcgggccg gacttcgggc
     9721 ccgatgacat cacctacaac cacgggatgc acggcgtaca ggacctgtgc aacgtgcttc
     9781 tgcgccacgg gcgggagttc cacctcatca ccgggcacta tcaggatgcg gcggcggtgg
     9841 ggaaggtgca cgacctgatc cgggccgcgg cg
//
LOCUS       DAOSPL010000008         9759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_6951_length_9759_cov_3.013293, whole genome shotgun sequence.
ACCESSION   DAOSPL010000008 DAOSPL010000000
VERSION     DAOSPL010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 9759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9759
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6951_length_9759_cov_3.013293"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PLQ54_00315"
     CDS             complement(<1..624)
                     /locus_tag="PLQ54_00315"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HOS91730.1"
                     /translation="MRDRLRDLLERLLVTHSPSGEEGEMEALCLAELEPLCSRVWQDE
                     ARNIIGLIEGRGEGPPVRLMAHKDELGCLVARIDDNGKMRLEPLGGAHAWVYGEGPMD
                     VLGTEIVTGVLGVGSKHVSDLSADVAAARSTKPLSWDVCRLDCKLDAKTLAAKGITVG
                     TRACVSRSRKTPVWMGDYVGGYAMDDKASVAAVLLAAESMRDARLKPR"
     gene            792..2789
                     /locus_tag="PLQ54_00320"
     CDS             792..2789
                     /locus_tag="PLQ54_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91731.1"
                     /translation="MALGERSHDRWLWIELIGFDNESPDLGVGDLLARTGFAPQAVSL
                     LFFTPDFVHTHAGMADEHVLPPDQCAYCARPLGRERKRQAWTNWQLKRLVEALHARGV
                     AVYCSFFNLFSSTVDGQFHQSPWCAAHPEMWETRRNGDRAAMLCPLKRMADGIEYEAF
                     FVPQLVQVMDDYGFDGYHGADGYSSTRLPLNEIDYSDDMVDQWRRASGVELPDSLRGP
                     CEKDPARCEERAAWIWANQRESWLEFWAERFAGFWSLVTSALHAKGKRVVLNSGWTRD
                     PFQAAYRYGADYQRLARAGVDGFVVETVSAGVSIGAEGILTDPRYDYQAMLLLMKGYT
                     PDVPLMCLNGTQDTCEQWDVLRHAPTASEREILSLSSLFLRRADGSLTRCSSGPVVCL
                     SDGIAAHEWQWLSDRWELGFAYRPTRTIGPTLVWSDAAHQAQLADYIATRRWTVHKTV
                     YELMAANAPIRTIARAEDLSGVTGPLLVVNPHLWPADELVRVRNYTGGPVMAIGGRTG
                     ALPAEAIVAEDGPDSMALVCWVLGGAASPPAAVGDGPGNVGWPTAPDPVTFLEELPYR
                     AVSAQFVQACARLIVQLSEAVEVVGRADVTRAWAVDLGEIQRLFITNDSHYYAAAEVD
                     LHRPIESVAVATCFPGMPVMPNGSRITVRVPPRGIVALDVR"
     gene            3074..4069
                     /locus_tag="PLQ54_00325"
     CDS             3074..4069
                     /locus_tag="PLQ54_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS91732.1"
                     /translation="MLKIAIVGMGNIGNVHARCYQANPNTEIVAVCDIIREKADRAAQ
                     EYGARAFYSVQEMLASGTTIDAASMCTRGEENGSEHFTPTMELLEAGIPTLGEKPISN
                     DVSEARRMVALAKKKGIPYGINLNHRFTPAARRAKEWLDEGRLGTLNIVNMTMWINNP
                     IETSPWFHIRALHPHSLDVMRYFAGDYAKAQCFFRKGEGRKIWSNLQANLLFRNGVLG
                     HLTGSYDAGGSYGLETLELVGSKGRVVINEACEKLWFYPRFSAEVECYEHLGGMGSFP
                     DTFGSRIGAWVDDLMKGTPPDQVDAKGEDALKVQLCIEACIESWKTNQVVTVKQK"
     gene            4122..4925
                     /locus_tag="PLQ54_00330"
     CDS             4122..4925
                     /locus_tag="PLQ54_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOS91733.1"
                     /translation="MIQLGVNTVLFGDRDLRTAMEHIKWAGYDAAEISALPGMGEHLP
                     LGNWKAVAGDIKAISEDLELPISATEVGSNTEDRLMPAYEACAEIGIPVINVGPGGYE
                     KDNPDHMARLIEQLNRMAEKAEPFGVTLCIKAHVNTAIYNTPSTLAIMEGVKSPAFGI
                     DMDPSHIHRAGEVPKDALRSVVSRVKHVHIRDCVGPGPSPGSPDQQACGRGEIDLLGY
                     CQVLVEAGYDGPVNLEIIGAKDWDLSRQAIIAAESYGYLNACLKMCGGR"
     gene            4956..6017
                     /locus_tag="PLQ54_00335"
     CDS             4956..6017
                     /locus_tag="PLQ54_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS91734.1"
                     /translation="MPLGVGILGFAHGHVNAYCSQWAQNDDMDIRVRAAWDHDAARLE
                     SNCRAFGLEACADPESLLARSDIQAVVIAAETSMHADLTERAAAAGKSIVVQKPMALT
                     LPEADRMVAAVQRAGVPFSMAWQMRVDPQNIQMRDLIQSGELGRVFMVRRRHGLSMCL
                     DKSFTTSWHVAPKYNRDIFADDASHPIDLIHWMLGVPETITAEIETLCHSEMPCDNGI
                     AVFRYSGGPLAEVVCSFSNPAGENTTEIVAEKGSVIQNYGDGVSCNVPRAGGPCGLKW
                     FLRQKGEWVFSDIESPTDHGARIHGLARPLSEFLHGRRPPIATAEEGRTSLRMLLACY
                     VSSREGRRVRFDDDAIARV"
     gene            6326..7735
                     /locus_tag="PLQ54_00340"
     CDS             6326..7735
                     /locus_tag="PLQ54_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOS91735.1"
                     /translation="MPKSPASIKDKPNVVLFGIDSLVSTHMSCYGYHHYTTPHIDEFA
                     EEGVLFENNFCPHVPTTSGYASMLTGRDCFGTNVVALRHHGPMSEKTKTLAEILGKVG
                     YETTCVGFTGNPASRGFHNYLDYVGWGSLEDGSLPKAGNLNDVAIPELERLAAGDKPF
                     FLMLRHMDPHSPYMPPKPFDRMFYHGNEFDKRNKSMKPVFDFVPFRDYFLTWMPPGVT
                     DKDYIIAQYDGAVAYMDACIKSFFMAMEDLGILDDTLVIINADHGETLYDHECWFDHH
                     GTYDVTLRVPLIMRYPRRLPAGLRVPGFTQHKDLVPTVMDLLDLKPRATFDGRSLMPM
                     VRGEVSMFESEFYITECTWMRKHGWRTPEWKLIVALEPDFHFKPPVELYNLAQDPDEL
                     NNVAEQEPGVVEMLRKRMDDFIAKREKEEGIRNPMFTQECWHGYPEVGPAFKSSEQAY
                     NTLHIGDPVKARELQAKAH"
     gene            8030..9025
                     /locus_tag="PLQ54_00345"
     CDS             8030..9025
                     /locus_tag="PLQ54_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS91736.1"
                     /translation="MLRVAVIGCGPIGNRHADIYKLDTLSELVGVCDIIKDRADAAAA
                     RLGVPAFYDAQTMIDALRPDICSVATGGIEYGSDHYEPTMQALSAGCHVLCEKPICNE
                     IDKAEEMVALARDKGLCFGIDLNHRFTPAARLAKTWVDQGLIGHQLFINMSMWIKNPN
                     ESSPWFMVKALNPHTVDVMRYFAGDIEAVHAFATKAPGRQIWSTMQMNARFVSGAVGN
                     LTASYDIERGHPMERCEVAGTLGRFVLDDMWREATYYPAGNPEKRVYTNPVFGGYRDF
                     VDTFIDRLTTFTRQVSEGTPPDQIDGSGADGLAAQKVLAAAIESVKTESVVRVSR"
     gene            complement(9048..>9759)
                     /locus_tag="PLQ54_00350"
     CDS             complement(9048..>9759)
                     /locus_tag="PLQ54_00350"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOS91737.1"
                     /translation="SPAAAAWLEEFVTAGGHALFVDGPVFAIKEPALQRVIGLAGTGE
                     YFNEFRAVIPAEGQELIKPGPPVDLDVEKRRAAAWEQFRRDSVTDLVRQVYAGAKALK
                     PEAQVSAAVFYDRRSADSVCQDWYGWLREGIIDYVLPMAYVDGLPPLEKAIDEWKAFD
                     PTLERIIPGLSIYRQEGDQTVSRDAASVLAQQELCRSSGARGTCYFSLEFLSPEIQAA
                     LAGGPYAEIAEPYYPGGR"
BASE COUNT         1719 a         3343 c         3077 g         1620 t
ORIGIN      
        1 ccgcggcttc aggcgggcat cgcgcatcga ctcggccgcc agcaacaccg ccgccaccga
       61 cgccttgtcg tccatggcat agcccccgac gtagtcgccc atccacacgg gtgtcttccg
      121 cgatcggctc acgcacgccc gcgtgccgac ggtgatgccc ttcgccgcca gggtcttcgc
      181 gtcgagctta cagtcgagcc ggcacacgtc ccacgagagc ggcttggtcg accgggccgc
      241 ggccacgtcg gccgagaggt cgctcacgtg cttgctgccc acgccgagta cgcccgtcac
      301 gatctcggtg cccaggacat ccatgggccc ctcgccatag acccatgcgt gcgcgccgcc
      361 gagtggctcg aggcgcatct tgccgttgtc gtcgatgcgg gccaccaggc agccgagctc
      421 gtccttgtgc gccatgaggc gcaccggcgg gccctcaccg cgcccctcga tcaggccgat
      481 gatgttccgc gcttcgtcct gccacacccg cgagcacaga ggctccagtt cggcgaggca
      541 cagggcttcc atctctccct cctcgcccga aggcgagtgg gtcacaagca ggcgttcgag
      601 caggtcgcgc agccggtctc tcatggtgtg agcgaacacc tcaccattct tgtcgtgccg
      661 cttcttcctg cggactcgct tcgcggctta aacgtcccag gcctgcaggt gggccgttga
      721 cagggccgaa gtccatcagg gcccgtgtgg atccaggcga cgtgttggca ttcggcagga
      781 gcgtggcagc gatggcattg ggggagcggt cacacgacag atggctgtgg atcgagctga
      841 tcggcttcga caatgagagc cccgatctgg gcgtcggcga tctgctggcg cggacgggct
      901 tcgcccccca ggccgtctcg ctgctgttct tcacgcccga cttcgtccac acccacgcag
      961 gcatggctga tgagcacgtg ctgccgccgg accagtgcgc ctactgcgca cggccgctgg
     1021 gccgcgagcg gaagcggcaa gcgtggacca actggcagct caagaggctg gtcgaggccc
     1081 tgcacgcccg cggcgtggcc gtctactgct ccttcttcaa cctgttctcg agcaccgtcg
     1141 atgggcagtt ccaccagagc ccctggtgcg cggcgcaccc cgagatgtgg gagacgcgcc
     1201 gcaacggcga tcgggccgcc atgctctgcc cgctcaagcg catggccgat ggcatcgaat
     1261 acgaggcatt cttcgtcccc cagctcgtgc aggtgatgga tgactacggc ttcgacggct
     1321 accacggcgc cgatggctac agcagcaccc ggctgccgct caacgagatc gactatagcg
     1381 atgacatggt cgaccagtgg cgccgcgcgt cgggcgtgga gctgccggat agcctgcgcg
     1441 ggccctgcga gaaggatcct gcccggtgtg aggagcgcgc cgcgtggatc tgggcgaacc
     1501 agcgcgaatc atggctcgag ttctgggccg agcgcttcgc cggcttctgg agcctcgtca
     1561 cgtcggccct gcacgccaag ggaaagcgcg tggtgcttaa cagcgggtgg acccgcgacc
     1621 ccttccaggc ggcgtaccgt tacggcgccg attaccagcg tctcgcccgc gccggggtcg
     1681 acggcttcgt ggtcgagacc gtgagcgcgg gcgtgtccat cggggccgag ggcatactca
     1741 cggacccccg ctacgactac caggccatgc tgctgctcat gaagggctac acgcccgacg
     1801 tgcctctcat gtgcctcaac ggcacgcagg acacctgcga gcagtgggat gtgctccgcc
     1861 acgcacccac ggcttcggag cgcgagatcc tttcgctcag cagcctgttc ctccgccggg
     1921 ccgacggctc gctgacgcgc tgctcgtcag ggcccgtcgt ctgcctctcc gacggcattg
     1981 cggcgcatga gtggcagtgg ctgagcgatc gttgggagct cgggtttgct taccggccga
     2041 cccggaccat cgggcccaca ctcgtctggt ccgatgccgc acaccaggct cagcttgccg
     2101 actacatcgc aacccggcgc tggacggtcc acaagaccgt ttacgagctc atggcggcga
     2161 acgcgccgat ccggaccatc gcccgcgccg aggacctatc gggcgtgacc ggcccgctgc
     2221 tggtcgtcaa cccgcacctc tggccagccg acgagctggt gcgcgtccgc aactacacgg
     2281 gcggcccggt catggctatc ggagggcgca ccggcgccct gccggccgag gctatcgtcg
     2341 ccgaggacgg gccggactcc atggcgctgg tctgctgggt cctcggcgga gctgcttcac
     2401 ccccagctgc agtgggtgac gggcccggga atgtgggttg gcccacggca cccgatccag
     2461 tgaccttcct cgaggagcta ccctatcgcg cggtgtcggc ccagttcgtc caggcctgtg
     2521 cccggctgat cgtacaactc agcgaggcgg tcgaagtcgt ggggcgcgcc gacgtcaccc
     2581 gcgcatgggc cgtcgatctg ggcgagatcc agcggctctt catcaccaac gatagccact
     2641 actacgccgc ggccgaggtc gatctgcacc gcccgatcga gtcagtggcc gtcgccacct
     2701 gtttccccgg catgcccgtg atgccgaacg ggtcgcgcat cacggtacgt gtcccgcccc
     2761 gcggcatcgt ggcgctggac gtgaggtagc gtcgtacagt ggcccacagc gggccgggca
     2821 gggccccgcc agccttgggc tggccggccg cctgctcgat ccctccgaac acatgggtga
     2881 cgcgaggagc ggccgaatgg aacagaccca cgcaccgcga aggatcccat ccggcccgcc
     2941 cgcgtgccga ggggatcggc cgccgagcgc gcgcgacctt gaatctcctt gcagttgggg
     3001 ctatgctccc gtcgaatgcg ccctctgaac cggcgtacca ttaccccccg cgcggtcccc
     3061 caaggagcgt cacatgctca agatcgccat cgtcggcatg ggcaacatcg gcaatgtgca
     3121 cgcacgctgc taccaggcga acccgaacac cgagatcgtt gccgtgtgtg acatcatcag
     3181 ggagaaggcc gaccgcgccg ctcaggagta cggtgcccgg gccttctaca gcgtccagga
     3241 gatgctcgcc agcggcacga ccatcgacgc cgccagcatg tgcacccgcg gcgaggagaa
     3301 cggcagcgaa cacttcaccc ccaccatgga gctgctcgag gcgggcatcc ctaccctggg
     3361 tgagaagccg atatcgaacg atgtcagcga agcccgccgg atggtcgctc tcgccaagaa
     3421 gaagggcata ccctacggca tcaacctcaa ccatcgtttc acgccggccg cccgccgcgc
     3481 caaggaatgg ctcgacgagg gacggctcgg gacgctcaac atcgtcaaca tgaccatgtg
     3541 gatcaacaac cccatcgaga cgagcccgtg gttccacatc cgcgccctgc accctcactc
     3601 gctcgatgtc atgcgctact tcgcgggcga ctatgcgaag gcccagtgct tcttccgcaa
     3661 gggcgagggc cggaagatct ggtcgaacct ccaggcgaac ctgctgttcc gcaatggtgt
     3721 gctgggccac ctgaccggca gctacgatgc cggggggagc tatggtctcg agaccctgga
     3781 gctcgtcggg tccaagggcc gagtcgtcat caacgaggct tgcgagaaac tgtggttcta
     3841 cccgcgcttc tcggcggagg tcgagtgtta cgagcacctc gggggcatgg gctccttccc
     3901 cgacaccttc gggtcacgca tcggtgcatg ggtcgacgat ctgatgaagg gcactccacc
     3961 cgatcaggtc gacgccaagg gcgaggatgc cctgaaggtc cagctctgca ttgaggcatg
     4021 tatcgagtcg tggaagacga atcaggtcgt caccgtaaag cagaagtagc tggccgcgcc
     4081 ctgggcgcgg acgtcaggct cctgagagaa gggtccgcat aatgatccag ttgggcgtca
     4141 atacggtgct ctttggcgat cgggacttgc gcaccgccat ggagcacatc aagtgggcgg
     4201 gctacgacgc ggccgagatc tccgccctgc ccggcatggg cgagcatctc cccctgggca
     4261 actggaaggc cgtggccggc gatatcaagg cgatctccga ggatctcgag ctccccatct
     4321 ccgccacgga ggtcggctcg aacaccgagg acaggctgat gcccgcctac gaggcctgcg
     4381 ccgagatcgg catacccgtc atcaatgtgg gaccgggggg ctatgagaag gacaaccccg
     4441 atcacatggc ccggctcatc gaacagctca accgaatggc ggagaaggcc gagcccttcg
     4501 gcgttaccct gtgcatcaag gcccacgtga acacggcgat ctacaacacc ccctcgaccc
     4561 tcgcgatcat ggaaggggtc aagtcccccg ccttcggcat cgacatggat ccgagtcaca
     4621 tccaccgcgc gggtgaggtg cccaaggacg cgctccggag cgtggtttcg cgggtgaagc
     4681 atgtccacat ccgcgactgc gtgggccccg gcccctctcc tggcagcccc gaccagcagg
     4741 cgtgtggccg cggcgagatc gacctactcg gctactgcca agtactcgtc gaagccggct
     4801 atgacggtcc cgtgaacctg gagatcatcg gcgccaagga ctgggacctc tcgcgccagg
     4861 ccatcatcgc ggccgagagc tacggctacc tgaacgcctg cctgaagatg tgcggcggcc
     4921 gctagggcca tgcgggccca ttcgaggtga ttggaatgcc cctgggtgta ggaatcctcg
     4981 gtttcgctca cggtcacgtg aacgcctact gctcgcagtg ggctcagaac gacgacatgg
     5041 acatccgggt ccgtgctgcg tgggatcacg atgcggctcg actcgagtcg aactgccgtg
     5101 ccttcgggct cgaggcgtgc gcggatcccg aaagcctgtt ggctcggtcg gacatccagg
     5161 ccgtggtcat cgcggcagag acgtccatgc acgccgacct gacggagcgg gctgccgccg
     5221 ctggcaagtc gatagtcgtg cagaagccca tggccctgac gctgcccgag gccgaccgca
     5281 tggttgcggc cgtgcagagg gcgggcgtgc ccttctccat ggcctggcag atgcgcgtcg
     5341 atccccagaa catccagatg cgtgacctca tccagagcgg cgagctcggc cgtgtcttca
     5401 tggtccggcg ccgccatggc ctgagcatgt gtctcgacaa gtccttcacc acctcgtggc
     5461 acgtcgcgcc caagtacaac cgtgatatct tcgccgacga tgcctcccac ccgatcgacc
     5521 tgatccactg gatgcttggc gtgcccgaga cgatcaccgc cgagatcgag acgctgtgtc
     5581 actcggagat gccctgtgat aacggcatcg ctgtgttccg gtactcaggg gggccgctgg
     5641 ccgaggtggt gtgctccttc tcgaatcctg ctggggagaa cacgacagag atcgtggccg
     5701 agaagggatc cgtcatccag aactacggtg acggcgtgag ctgcaacgtg ccccgagcgg
     5761 ggggcccgtg cgggctcaag tggttcctgc gtcagaaggg cgagtgggtt ttcagcgaca
     5821 tcgagagccc gaccgaccac ggtgcgcgga tccacggcct cgcccggccg ctgtccgagt
     5881 ttctgcacgg caggcgcccg cccatcgcta cggcggagga ggggcgcacg tcgctccgaa
     5941 tgctcctggc atgctatgtt tcatcgcgcg aagggcgccg cgtccggttc gacgacgacg
     6001 ccatagctcg ggtctaggca acccgggccg gatggggccc caccggactt cggctggtcc
     6061 ggtctccatg tggcccctcc gaacagcgcc gcagggcaca aggagcgtcg gcatggaaga
     6121 ggcccgcaga gcggcaggga ctccacccga gccgctccac ggaacggagg gacgccgccc
     6181 tgagcccgtc gaggggctgc gcactgatgg atcgagcggg gcgggccccg gcccgacacg
     6241 agacaacggc aaacgagcaa tcaccacacg ccgccgggcg gcgtggtctc agcccggact
     6301 actgtgagcg gaaggaacga agcccatgcc caagagccct gcctcaatca aggacaagcc
     6361 gaatgtcgtc ctgttcggca tcgactcgct ggtctcgacc catatgagct gctacggcta
     6421 ccaccactac accacgcccc acatcgacga gttcgccgag gagggtgtgc ttttcgagaa
     6481 caacttctgc ccccacgtcc ccaccacgtc gggctatgcc tccatgctga ccgggcgcga
     6541 ctgtttcggc accaacgtcg tcgctctgcg ccaccatggc ccgatgtccg agaagaccaa
     6601 gactctggcc gagatactgg gcaaggtcgg gtacgagacg acctgtgtgg gtttcaccgg
     6661 aaacccggcc tcgcgcgggt ttcataatta cctcgactac gtgggctggg gctcactcga
     6721 ggacggttcg ctgccgaaag ccgggaacct caacgacgta gccattcccg agctcgagcg
     6781 gctcgcggcc ggcgacaaac ccttcttcct gatgcttcgg cacatggatc ctcactcccc
     6841 ctacatgccg cccaaaccct tcgaccggat gttctaccat gggaacgagt tcgacaagcg
     6901 gaacaagtcc atgaagcccg tcttcgactt cgttcctttc cgcgactact tcctgacctg
     6961 gatgccccca ggcgtcaccg ataaggacta tatcatcgcc cagtacgacg gggctgtggc
     7021 ctatatggat gcgtgcatca agtccttctt catggccatg gaggacctgg gcatattgga
     7081 tgacaccctg gtcatcatca atgctgacca cggcgagacg ctctatgacc acgagtgctg
     7141 gtttgaccac cacggcactt acgatgttac gctccgcgtg ccgctcatca tgcgctatcc
     7201 gcgccggctg cccgccggcc tgcgtgtacc cgggttcact caacacaagg acctagtccc
     7261 caccgtgatg gacctgctgg acctcaagcc cagggctacc ttcgatggcc ggtcactcat
     7321 gcccatggtt cggggcgagg tcagtatgtt cgagtccgag ttctacatca ccgaatgcac
     7381 gtggatgcgc aaacacgggt ggaggactcc cgagtggaag ctcatcgttg cactcgaacc
     7441 cgactttcac ttcaagccgc ccgtcgagct ctacaacctg gcccaagacc ccgacgagct
     7501 caacaacgtg gccgagcaag agcccggtgt tgtcgagatg ctccgcaagc gcatggatga
     7561 cttcatcgcc aagcgcgaga aggaggaggg catccggaat ccgatgttca cccaggagtg
     7621 ctggcacggc taccccgagg tcggcccggc gttcaagtcc agtgaacagg cctacaacac
     7681 gttgcatatc ggggatcccg tcaaggctcg ggaactccag gccaaggcac actagcgagc
     7741 caaggcgtcg acccgcccta tcttgcggag ggaacgcact ggggggacca cgcgccggca
     7801 ggcgggtggt ggggccctga ccggcccggt cggctagagg ctgacctcat cagggcagag
     7861 atgggcgcgc tgggggccca ccacgcaccg agcccgcgcg gatcccccca tggcaccccg
     7921 ccgcaacccg ggttgctcaa ggccaggggc gtcgttgccg ccagtacctg gcggcggcct
     7981 ctcacggggc agtgagccct gcgagtccag ctagggagtt gcaccaacca tgttgcgagt
     8041 ggcagtcatc gggtgcggtc ctatcgggaa ccgtcacgcc gatatttata agctcgatac
     8101 ccttagcgaa ctcgtgggag tgtgtgacat catcaaggac cgcgccgatg cggctgctgc
     8161 tcggctgggt gtacccgcct tctacgatgc acagacaatg attgatgcgc tccgccctga
     8221 catctgcagc gttgccacag gcggcatcga gtatggcagt gatcactatg agcctaccat
     8281 gcaagcccta tcggccggct gccacgtcct gtgcgagaag cccatatgca atgagatcga
     8341 caaagccgag gaaatggtcg cattggcccg agacaagggt ctctgcttcg gcattgacct
     8401 gaaccaccga ttcacgccgg cggcgcggct cgccaagacc tgggtcgatc aggggctcat
     8461 cggccatcag ctcttcatca acatgtcgat gtggatcaag aaccccaacg agtcgtcgcc
     8521 ctggttcatg gtcaaagccc tcaacccgca cacggtcgat gtgatgcggt atttcgcggg
     8581 cgacattgaa gccgtccatg ccttcgcgac caaggcgcca ggacgccaga tctggtccac
     8641 catgcagatg aacgcccgct tcgtcagtgg cgctgtgggg aacctgacgg cgagctacga
     8701 catcgagcgc gggcacccca tggaacgctg cgaggtggct gggaccctcg ggcgattcgt
     8761 gctggacgat atgtggcgtg aggctaccta ctaccccgcc gggaacccgg agaagcgagt
     8821 ctacaccaac cccgtttttg gtggctatcg cgacttcgtc gataccttca tcgaccgcct
     8881 gacgactttc acccgccagg tgtccgaagg caccccgccc gatcagatcg acggctccgg
     8941 cgccgatggt ctcgctgccc agaaggtcct cgcggccgct atcgagtcag tgaagacgga
     9001 atccgtcgtc cgcgtcagcc gttagtagac ggtcgcccgg ccccgcccta ccgccccccc
     9061 gggtagtacg gctccgcgat ctccgcatac gggcctccgg ccagcgccgc ctggatctcc
     9121 gggctcagga actccagcga gaagtagcac gtcccccggg cccccgacga ccggcacagt
     9181 tcctgctgtg ccagaaccga tgccgcatcg cgcgacaccg tctggtcacc ctcctgcctg
     9241 tagatggaca acccggggat gatccgctcc agcgtcgggt cgaaggcctt ccactcatcg
     9301 atcgccttct ccagtggggg caggccatcg acgtaggcca tcggcaatac gtagtcgatg
     9361 atcccctccc gcagccagcc gtaccagtcc tggcacaccg agtcggccga ccgccgatcg
     9421 tagaacaccg ccgcgctgac ctgcgcctcg ggcttcagcg ccttggcgcc cgcgtagacc
     9481 tgccgcacca ggtcggtcac cgaatcgcgc cggaactgct cccacgccgc cgcccggcgc
     9541 ttctcgacat ccagatcgac cggcgggccc ggcttgatga gttcctgccc ctcggccggg
     9601 atgaccgcgc ggaactcgtt gaagtactcc cctgtgcccg cgagtccgat cactcgctgc
     9661 agcgccggct ccttgatggc gaacacgggg ccgtcgacga agagcgcatg gccgccggcc
     9721 gtcacgaact cctcgagcca cgccgccgcc gccggcgag
//
LOCUS       DAOSPL010000009         9293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_7363_length_9293_cov_3.394025, whole genome shotgun sequence.
ACCESSION   DAOSPL010000009 DAOSPL010000000
VERSION     DAOSPL010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7363_length_9293_cov_3.394025"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1230)
                     /locus_tag="PLQ54_00355"
     CDS             complement(16..1230)
                     /locus_tag="PLQ54_00355"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOS91738.1"
                     /translation="MPSVGSGPPRGRRALIAFSLLLGTSWWVGCARSGSGGATHEQEH
                     GAPASAHTGEGTAALPPGTVTEPFDGQELAADQLLLLRTSRGDLYIEVDARECPVGAS
                     HFVRLAREGYLAGRMFDRAVRGRSVHAGAKPEGAKLKDPDWRIVAERSPQRALRGYVF
                     LDRDASSPELAEAVGFRILRARDVLLEDGEQSQGGIPMSYVGRVVKGLAVVDAIEEGD
                     TIQAVSVVTRQAMGKPDAGRMLLDPGASRTTLATPKAVSPEWRLRTMDGQEYSADSTL
                     GRALVLTFVESGNKASEALMDALQEIRDRVAETDLLILVVAPVDQDENALRAWAKRGG
                     WTLPLVSGRSPRVLDVTASFMADQVPLTWIVDSTGVAVEKIAGGHPAPVLLARLELVG
                     VRARDATRAPGR"
     gene            complement(1319..2374)
                     /locus_tag="PLQ54_00360"
     CDS             complement(1319..2374)
                     /locus_tag="PLQ54_00360"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOS91739.1"
                     /translation="MRIGLVGLPGSGKTTIFNLLTGLKKDLDGSTHGRTEPNVGVLKV
                     PDPRLERLAEITESKRKVQAEVALVDVLGARPSRGASGLGAEDVARHVEDADAFCVVI
                     GAFAVDGPGAVAQLEDVLLSLALADLAKVERRLERVLRDIQRGKKEGERERALLEHVR
                     APLEAGVGLAKALPAERDRVELRGFQFLSAKPVAVLENVRDDDAGTDGALADVCRLQR
                     YPHTVVAGRLELEIAELPPEEQLGFLSEYGIRSSARERCISSVYEALDAVTFYTIAHG
                     EARAWPVRSGTVALDAAGAVHSDMKAGFIRAEVIPFDVLAEVGSLAECRARGVVRLEG
                     RDHVVHDGDIITFRFSS"
     gene            2729..3478
                     /locus_tag="PLQ54_00365"
     CDS             2729..3478
                     /locus_tag="PLQ54_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91740.1"
                     /translation="MTLRGWTFGFIAIVFALGATGAYAQVDKEKADEAVKFWVEQSKL
                     QQKVQELRAELWGLQAAGADAEKVQSKQEALTKAVDEVREHAAQRPAAIGACPYGGPG
                     IGAGFGRGAGFGRGRGGWQARVESVQDPELKQQITDLHNQIRAKQQELRLLTAQGKDT
                     AAVAAELKALREKLQAANDKAGLCIGVGPRAYGMDGAGGPGRGWGRGAGRAGGRGGFG
                     RGGGYGMGFGAGLGYGPGAGYGPYCPWGAAN"
     gene            complement(3730..4899)
                     /locus_tag="PLQ54_00370"
     CDS             complement(3730..4899)
                     /locus_tag="PLQ54_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012308642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOS91741.1"
                     /translation="MPTPQRMTESVRAGRYVPQPGGYRAFVPNPLPPVPPLRFGRDLQ
                     ALLSQADVALGRLDGSIQTLPNPDLFVLMYVRKEAVLSSQIEGTQSSLQDVLAAEAQV
                     LAPDRPKDVAEVINYVSAMNYGLERLRTLPISVRLFREIHERLVRNVRGARSSPGHIR
                     STQNWIGPPGCSLSEAAFVPPPPHEVAQALGDLEKFLHASAPPPLLVSIGLAHAQFET
                     IHPFLDGNGRIGRLLITLLLCERGVLARPVLYLSHFFRRHRQEYYDRLQAIRDAGDWE
                     AWLEFFLRGVAEVSQQSAGTARRVLGLREEHRSAITRSLGRGAAGGHRVLEYLFDHPI
                     VSVGELREVLNISYQGANQLVARLQQLGILQECTGRSRNRRFRYASYIELFTEGD"
     gene            5094..7139
                     /locus_tag="PLQ54_00375"
     CDS             5094..7139
                     /locus_tag="PLQ54_00375"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOS91742.1"
                     /translation="MLALTVALAPMCVFAAVDEIPVERLAEIEPGAQVQGRLVIREGN
                     VVIDGRGATLIGPGEDGSPDSLAEAGIGIEANGVSNVVLRNLNVHGFATGLQAHDCAN
                     WAIEGCDFSDNYHNPQFGWGEMPDRGGIALVQCRNFVLRGNRANRVWNGLSLTRCSDC
                     VVDANDFSHCSNVCAKLWESSRNAFTDNNLSYGIRIDREAGEVHARDSTCVLIESGSN
                     DNTFTRNDIRYGGDGVFVRVLNGWVSSGNVFTENDMSYANNNCVESWSPGNTYIRNIA
                     NHGSYGFWLGGSDRTVLIDNVAAYNGLPDGNHNAPEPGFEHGGIVIVSGSSSHTVLDG
                     NHCHHNNGAGIVFRGDVGSEGRAWRTYHWVIRRNRLHDNRFGIWGQWGDWITLAANVF
                     EDNGTDVVIQDTTRLVELPAGPKALRPPRAVLRGPGPVARVGRPVTFDASASRDPDGR
                     KLEFDWDIGDVRAQGPKVTHVFDRPGFYRVGVTVSNGTLADLAYADLVVGETGVTEIG
                     TEGQAERWGFAFEIDGEGRGRMEFGDDADAVCGRRSLRLRADPYPGGDATAFFPATRD
                     LGLDIAGRSRLVLWVRAENPNIPGFQDAGPVVALEGPEGVVVYSPDHGRNLLSSPPDS
                     EARWMWQRLSIPLDGGEGWVRSEGGPQSLSRVGAVSLTLDSWGGEPFTVWVDGLHFE"
     gene            7195..8208
                     /locus_tag="PLQ54_00380"
     CDS             7195..8208
                     /locus_tag="PLQ54_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOS91743.1"
                     /translation="MDGRIVLFEIGPWDAGSGDCAACEALKPWPTECSIGTLQSASGS
                     HPGVVCASVFVGSRVAADELARFPDLKLIATRSTGYDHIDLAACRERGVAVANVPAYG
                     ENTVAEHTFGLILALSRRITEAHERVQRGHFGVEGLKGFDLYGKTLGVIGAGAIGLHV
                     IRIGRALGMRCLAYDVRPVPLIAEVLGFEYASLDTLLAESDVVSLHAPLVPATRHLIR
                     RETVAQMKDGALLINTARGELVETAALIEALDSGKLRGVALDVFEGEALVAEENLLLR
                     RQAPPEQLQSALETRALLRRENVVLTPHIGFYSEEALGRIMETTIGNIRGFLSGEPRN
                     LVG"
     gene            8264..>9293
                     /locus_tag="PLQ54_00385"
     CDS             8264..>9293
                     /locus_tag="PLQ54_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91744.1"
                     /translation="MHSLWLVLAVAPWVTAYEEVAVSAGPEQVGVAWAAQLLADQCRV
                     PLRLSAAEAGPRAIRGGLFGDALVDDVRPAGVDGLSDQGFGWASGDVGIGRVARDPVG
                     LMYGLLELRDRLHNGEPIATEGTSEPFLKLRGDYIDLPFYLGCDLYDGRWRWHKDIEA
                     SPTSWFHDRAAWTRRLRTYALRRVNSIELQHPHPFPAFITYPDTPEAAYFDDATVARN
                     AETLQWLIDEAARYGIGLYIQTWNEWIPRGYAEAHNLPQEAPATPESAALNRYSYQEL
                     FRRFPGLAGLVTMAAESPPGCVGFVRDHVVKPLAELPDRPKIVYWTWCSFPEDVNVVL
                     DGYPGETAI"
BASE COUNT         1532 a         3126 c         3156 g         1479 t
ORIGIN      
        1 agcgggtccc gcgcctcacc ggcccggcgc tcgtgtggcg tcccgggctc ggacgcccac
       61 gagctccaat cttgccagca acaccggcgc cggatggccg ccggcgatct tctcgaccgc
      121 cacccctgtc gagtccacga tccacgtcag cggtacctgg tccgccatga acgaggcggt
      181 gacgtccaga actcgcggcg atcggcccga gaccagtggt agggtccatc cgccccgctt
      241 cgcccaagct ctcagagcgt tctcgtcctg atcgacgggc gccaccacca ggatcagcag
      301 atcggtctcg gccacacggt ctcggatctc ctgcagggcg tccatcagcg cctcggacgc
      361 cttgttgccc gactcgacga acgtgagcac cagggcgcgc cccaaggtgg agtccgccga
      421 gtactcctgc ccgtccatcg tgcgcagccg ccactcaggc gacacggctt tcggcgtcgc
      481 cagcgtggtg cgcgatgcac ccggatccaa gagcatcctg ccggcgtccg gcttacccat
      541 ggcctgacga gtgaccacgc tcacggcttg gatggtgtcg ccctcctcga ttgcgtccac
      601 caccgccagc cccttcacca ctctccccac ataggacatg gggatcccgc cctgggactg
      661 ctccccgtcc tccaagagca cgtcccgcgc ccggagaatc cggaagccca ccgcctctgc
      721 gagctccggg gatgacgcat cgcgatccag gaacacgtag ccccggagcg cccgctgtgg
      781 ggatcgctca gcaacgatcc tccagtcagg gtccttcagc ttcgccccct cgggcttcgc
      841 gccggcgtgt accgaccggc cgcgcaccgc ccggtcgaac atgcggcccg ccagatagcc
      901 ctcgcgcgcc aagcgaacga aatgcgaagc gcccaccgga cactcgcgcg cgtccacctc
      961 gatgtacaag tcgcctcgcg acgtcctcag gagcaggagt tgatccgccg caagctcctg
     1021 gccgtcaaac ggctcggtga cggttcccgg gggcagcgcc gcggtgccct ctcctgtgtg
     1081 cgccgacgcc ggtgcgccat gctcctgctc gtgagtggcc ccgcccgacc ccgaccgcgc
     1141 acatccgacc caccagctcg tgcccagcag aagagagaac gcgatgagcg cccggcgacc
     1201 ccgcggtgga cccgacccga cgctgggcaa tggtcgccac gtccatgaag tcactcccat
     1261 gctcgcacca ctccgcgatg agtcggcagc caagggattc ggggggtccc agggccgcct
     1321 atgagctgaa ccggaacgtg atgatgtccc catcgtgaac gacgtggtca cggccctcga
     1381 ggcgaaccac gccccgggcc cggcattcgg cgagcgaacc cacctcggcg agcacgtcga
     1441 aagggatcac ctccgcccgg atgaaacccg ccttcatgtc cgagtgaaca gctcccgccg
     1501 catcgagcgc taccgtgccc gaccgcacag gccacgctct cgcctcaccg tgcgctatgg
     1561 tgtagaacgt gaccgcgtcg agcgcctcgt acaccgagga gatgcagcgc tcccgggccg
     1621 aggaccgtat gccgtactcg gacagaaagc cgagctgctc ctcgggcggc agctcggcga
     1681 tctccagctc gagccgtcct gcaaccaccg tgtgcggata cctctgtagc cggcagacgt
     1741 cggccagcgc tccgtcggtg cccgcgtcgt catcacggac gttctccagc acggcgaccg
     1801 gcttggccga caggaactgg aaccctcgga gctctacccg gtcacgctcc gcggggagcg
     1861 ccttcgccag cccgacgcct gcctccaggg gtgcccggac gtgctcgagg agtgcccgct
     1921 cccgttcccc ttccttcttg cctcgctgga tgtcccgcag gacacgttcc agccggcgct
     1981 cgaccttggc caggtctgcc agggccaggc tcagcaacac gtcctccagc tgagccaccg
     2041 cgccaggacc atctaccgcg aaggcaccga tcaccacgca gaacgcatcg gcgtcctcga
     2101 catgacgggc gacgtcctcc gcccccaggc cgctggcgcc ccgggacggc cgcgcgccca
     2161 acacatccac cagggccacc tcggcctgaa ccttgcgttt gctctcggtg atctcggcga
     2221 gacgctccag acggggatcc ggtaccttca gcacacccac gttgggctcg gtcctcccgt
     2281 gggtcgagcc gtccaggtcc ttcttcagac ccgtcagaag gttgaagata gttgtcttcc
     2341 cactacccgg tagacccacc agccctatgc gcacggcaca tccgccgccc agcggccctg
     2401 gtcctctccg cgaatccacc ccgcccaagc gggtgcggat cccctcgaag ttcgccggaa
     2461 gtatacccca tcccctatcg cctcccggaa aagcgcccgg gaagcgacag ggccgcctac
     2521 cgatgcgcag gttcccccgg agtggcgggc ggcgccgtcc attcgcggtg accacccggc
     2581 ccacgctcga gagcgagatg accctggggg gcggcggcac tcggctccga cccggcactg
     2641 caaaatggga cggaatccgc gggcatcgcc gccgtcttgt aggtgcgccg cgcgaagcgg
     2701 catatcgaga atgtgggagg gatcgaccat gacactccga ggatggacgt tcgggttcat
     2761 cgcgatcgtg ttcgcactcg gcgccacggg tgcctacgca caggttgaca aggagaaggc
     2821 cgacgaggcc gtcaagttct gggtggagca gtcgaagctc cagcagaagg tccaggagct
     2881 tcgtgcggag ctgtggggcc ttcaagcggc cggggccgac gcggagaagg tgcagagcaa
     2941 gcaagaggcg ctcacgaagg ccgtggacga ggttcgtgag cacgccgccc agcgacccgc
     3001 agccatcggg gcgtgtccct acggtggccc agggatcggt gcgggcttcg gccgaggagc
     3061 gggcttcggg cgtggccgag gcggctggca ggctcgggtc gagtcggttc aggaccccga
     3121 gctcaagcag cagatcaccg accttcacaa ccagatccgc gcgaagcagc aggagctgcg
     3181 cctgctgacg gcccagggca aggacacggc cgcggtggcc gccgagctca aggcgctgcg
     3241 tgagaagctc caggcggcca acgacaaggc cggtctgtgc atcggcgtcg ggccgcgggc
     3301 ctacggcatg gatggtgcgg gcggcccggg ccgcggctgg ggtcgcggtg ctggccgcgc
     3361 cggaggacgc ggtggcttcg ggcgcggcgg cgggtatggc atggggttcg gagcgggcct
     3421 gggctacggt ccgggtgccg ggtatggccc ctactgcccc tggggcgcgg cgaactagcc
     3481 gcatcggatg acccgtcaac gttagcagcg atcacggcga cgcagagccc tcgcgagccc
     3541 caagctcgcg ggggctccgt ctgtgcgacg cggcgtgagt gccgccccgg tctcccccca
     3601 tatcaaactg acagttacac atggaggacc cggaagctct acgttcaggt ctggttgtgt
     3661 atgagggctt ccgggtggca ccaccaggct gagcgcggca gcaaggccct ctcccgtctg
     3721 cgcaagtggc tagtcgccct cggtgaagag ctcaatatag ctagcatagc ggaaccggcg
     3781 gttccggctg cggccggtgc actcctgcag gatcccgagt tgctgcagcc gcgcgacaag
     3841 ctggttggcg ccctggtaag agatgttgag tacctcacgg agttcgccaa cggacacgat
     3901 tgggtggtcg aagaggtact ccagcacgcg gtgcccgccg gccgcaccgc gcccaaggct
     3961 tcgggtgatg gcgcttctgt gctcctcgcg gaggcccaaa acgcgccgtg ccgtgccggc
     4021 agactgctga ctcacctccg cgactccgcg gaggaagaac tcgagccatg cttcccaatc
     4081 ccccgcatcg cggattgcct gcagccgatc gtagtactcc tggcggtggc gccggaagaa
     4141 gtgcgatagg tagagcactg gtctggcaag cacgccgcgc tcacacagca gaagagtgat
     4201 gaggagccgc ccgatccggc cattgccgtc tagaaaaggg tggatcgtct cgaactgggc
     4261 gtgagccagg cctatgctga ctagcagagg cggcggggcg ctggcgtgga ggaacttctc
     4321 gaggtcgcca agagcctggg cgacctcgtg gggtggcggg ggcacaaacg ccgcttcgga
     4381 cagggagcac ccgggggggc cgatccagtt ctgcgttgac cggatgtggc ccggtgacga
     4441 tcgggcgccc cgcacattcc ggacgaggcg ctcatggatc tcgcggaaca gccgaacgga
     4501 gatcggcaga gtcctgagcc gctcgagccc gtagttcatg gctgatacat agttgatcac
     4561 ctctgctacg tccttgggtc gatccggcgc cagcacctgt gcctcggcag caagcacgtc
     4621 ctggagagag ctctgtgtgc cctcgatctg gctcgaaagc accgcctcct tgcggacgta
     4681 catgaggaca aagaggtccg ggttcggcag cgtctggatc gagccatcga gccgcccgag
     4741 tgccacgtcg gcttgtgaga gaagtgcttg cagatcgcga ccgaagcgca gaggcggcac
     4801 tggtggcaga gggttgggta cgaaggcacg gtaaccacca ggctggggca cgtagcgccc
     4861 agctcgtacc gattcggtca ttctctgagg agtgggcata tacttgcctc cttctagcgc
     4921 tgcccccgcc ctgccacgaa tggcgcgccg ccacctccat actaaactgg cagtttagca
     4981 tagacgcccc gactgttcca tgctcaggtc tggttgcgta tggaggcctt ggccgaaggc
     5041 ctcggcccgg tctccggagt atcgtgaacc aatccgttct gtggaggcag cccatgctgg
     5101 cccttaccgt ggcgctcgcg ccgatgtgtg tctttgccgc cgttgatgag atccccgtcg
     5161 agcgtctcgc cgagatcgaa ccgggcgcgc aggtccaggg gcggctggtg atccgtgaag
     5221 gcaacgtagt tattgatggc cgtggggcca cgctgattgg gcctggcgaa gacgggagcc
     5281 ccgactcgct ggcggaagca ggcatcggca tcgaggccaa tggcgtctcg aatgtggtgc
     5341 tgaggaacct gaacgtgcat ggtttcgcca caggactgca ggcgcatgat tgcgcgaact
     5401 gggccatcga aggttgcgac ttctccgaca actaccacaa tccccagttc ggctggggcg
     5461 agatgcctga ccgcggcggg atcgcgctcg tccagtgccg caacttcgtg ctgaggggca
     5521 acagagccaa ccgcgtctgg aacgggctat cgctgacaag gtgctcggac tgcgtcgtgg
     5581 acgcgaacga cttctcgcac tgctcgaacg tctgcgccaa gctctgggag tcgagccgga
     5641 acgcgttcac ggacaacaac ctctcgtacg ggatccgcat tgaccgcgag gcgggtgagg
     5701 tccacgcccg ggattccacg tgcgtgctca tcgagagcgg ctcgaacgac aatacgttca
     5761 cccggaacga catccgctac ggcggcgatg gggtgttcgt ccgggtgctc aatggttggg
     5821 tgagcagcgg caatgtgttc accgagaacg acatgtcgta cgccaacaac aactgcgttg
     5881 agtcgtggag cccggggaac acctacatcc gcaacatcgc caaccacgga tcgtacggct
     5941 tctggctggg cggcagcgac cgcacggtgc tcatcgacaa cgtcgcagcc tacaacggcc
     6001 tgcccgacgg gaaccacaac gcgcccgagc cggggttcga gcacggtggg atcgtgatcg
     6061 tcagtggctc atcgagccac acagtcctgg acggcaacca ctgccatcac aacaacggcg
     6121 cggggatcgt cttccggggc gacgtgggca gcgaggggcg cgcgtggcgg acgtaccact
     6181 gggtgatcag gcgcaaccgc cttcacgaca accggttcgg catctggggg cagtggggtg
     6241 actggatcac gctggcagcc aacgtgttcg aggacaacgg caccgacgtc gtcatccagg
     6301 acaccacgcg cctggtcgag cttccggcag ggcccaaggc actccgcccg cctcgggccg
     6361 tgctccgcgg gcccggaccg gtggcgcgag tggggcgtcc ggttaccttc gacgcttcgg
     6421 cgagccggga tccagatggc cggaagctgg agttcgactg ggacatcggc gatgtgcgcg
     6481 cccagggccc gaaagtgacc cacgtgttcg atagaccggg cttctaccgg gtgggcgtga
     6541 ccgtcagcaa cgggacactc gccgatctcg cgtacgccga cttggttgtc ggagagactg
     6601 gggtgaccga gatcgggacc gagggacaag cagagcgttg ggggttcgcc ttcgagatcg
     6661 acggcgaggg acgcggacgg atggagttcg gcgacgacgc tgatgccgtc tgtgggcgcc
     6721 gctcgctgcg cctgcgcgcc gacccgtacc cgggtgggga tgccacggcc ttcttccccg
     6781 cgacccgcga cctcggactc gatatcgcgg gccggtcgcg cttggtcctg tgggttcgcg
     6841 cggagaaccc caacatcccc ggcttccagg acgccggtcc cgtggtggca ctcgaggggc
     6901 ctgagggcgt tgtagtgtac agcccggacc atgggcggaa cctgctctcg tcgccgccgg
     6961 acagcgaggc ccggtggatg tggcagcgcc tctcgatccc gctcgacggt ggcgagggat
     7021 gggtgcggag tgagggtggg ccgcaatcgc tgtcgcgagt cggcgcagtg tcgttgactc
     7081 tcgactcctg gggcggcgag cccttcacgg tctgggtgga cgggctccac ttcgagtagc
     7141 tcctctaccg ttggggctgc tcggcgacgt tctgacaagg agtgccccat gcggatggac
     7201 ggacgcattg tgttgtttga gatcggcccc tgggacgcgg gcagcggcga ctgtgcggcc
     7261 tgcgaggccc tgaagccttg gcccacggag tgctcgatcg ggacgctcca gtctgcatcg
     7321 ggcagtcacc ccggcgtggt gtgcgcgtcg gtgttcgtcg ggtcccgtgt cgcagcggac
     7381 gagttggcgc gcttccccga cctgaagctg atcgcaaccc ggtcgaccgg gtacgaccac
     7441 atcgatctgg cggcgtgccg ggagcgtggc gtggcagtcg ccaacgtgcc cgcgtacggc
     7501 gagaacacgg tggcggagca caccttcggg ctgatcctcg cgttgtcgcg gcgcatcact
     7561 gaggcccacg agcgggtgca gcggggtcac ttcggagtcg agggcctcaa gggcttcgac
     7621 ctgtacggca agaccttggg cgtcatcggg gcgggcgcca tcgggctgca cgtgatccgc
     7681 atcggtcgcg cgctgggcat gcggtgcctg gcctatgacg tgaggccggt tccactcatc
     7741 gccgaggtgc tgggcttcga gtatgcctcg ctggacacgc tactggccga gtccgatgtc
     7801 gtctcactgc acgccccgct tgtgccggcg acgcggcacc tcatccgccg cgagacggtc
     7861 gcgcagatga aggatggcgc cctgctgatc aacacggccc ggggtgaact ggtcgagacc
     7921 gcggcgctga tcgaggccct cgacagcggc aagctccgcg gagtagcgct ggatgtgttc
     7981 gagggcgagg cgctggtggc cgaggagaac ctcctgcttc ggcgccaggc accgcccgag
     8041 cagctccagt cggccctgga gacgcgggcc ctgctccgcc gtgagaacgt ggtgctgaca
     8101 ccgcacatcg ggttctactc ggaggaagca ctgggccgga tcatggagac gaccatcggc
     8161 aacatccgag ggtttctcag cggcgagccg cggaacctgg tggggtgagg acggccgcag
     8221 gctgcccgcc ggccctcgcc gaacctgccg cggggtgatc gacatgcatt cgctgtggct
     8281 tgtgctggcc gtggcaccgt gggtgacggc gtatgaggaa gtggccgtgt ccgccgggcc
     8341 ggagcaggtg ggcgtggcat gggccgctca gctcctggcc gatcagtgcc gcgtgccgct
     8401 gaggctctcc gccgccgagg ccgggccgcg ggccattcgc gggggcctct ttggtgacgc
     8461 cctcgtagac gatgtgaggc ctgccggagt ggacggtctg tcggaccagg gcttcggctg
     8521 ggcgtccggc gatgtcggta tcggccgggt ggcgcgagac cccgtggggc tcatgtatgg
     8581 cctgctggag ctccgcgatc ggctgcacaa cggcgagccc atagccaccg agggtacatc
     8641 ggagccgttc ctcaagctcc gaggtgatta cattgacctg cccttctacc tgggctgcga
     8701 tctctacgac ggccgttggc gctggcacaa agacatcgag gcctcgccca cctcgtggtt
     8761 ccacgatcgc gccgcatgga cgcggcgact gcggacctac gcgctccggc gcgtgaacag
     8821 catcgagctc cagcatccac accccttccc ggcgttcatc acctacccgg acacaccgga
     8881 ggcagcctac ttcgacgacg ccacggtggc gcggaacgcc gagacgctgc agtggctcat
     8941 cgacgaggcc gcgcggtacg gcatcggtct ctacatccag acatggaatg agtggatccc
     9001 ccgcggatac gccgaagctc acaacctgcc ccaggaagcg ccggcgacgc cggagtcagc
     9061 ggcgctgaac cgctactcct accaggagct gttccgcagg ttccccgggc tggccgggct
     9121 cgtgaccatg gccgccgaga gcccgcccgg gtgtgtcggc ttcgttcgcg accacgtcgt
     9181 gaagccgctc gccgaactgc cggaccggcc caagatcgtg tactggacct ggtgctcgtt
     9241 ccccgaggat gtgaatgtgg tcctcgacgg ctatccgggc gagaccgcga tca
//
LOCUS       DAOSPL010000010         8974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_7741_length_8974_cov_3.051127, whole genome shotgun sequence.
ACCESSION   DAOSPL010000010 DAOSPL010000000
VERSION     DAOSPL010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8974
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7741_length_8974_cov_3.051127"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PLQ54_00390"
     CDS             complement(<1..354)
                     /locus_tag="PLQ54_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91745.1"
                     /translation="MRTGFTATGTVMWVAVVSALAASGLRAAAPDDSALASLRQCEPV
                     AEAVPGPYRVRCTIGAPSTWPAGAGVAVVYNRTSPTDGSVVLVGPGRVRHFRIGGGKV
                     LSRAESAPVALGGQAP"
     gene            complement(421..1560)
                     /locus_tag="PLQ54_00395"
     CDS             complement(421..1560)
                     /locus_tag="PLQ54_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91746.1"
                     /translation="MRRRFVSVLVFASATLSAQLAAAQPPGAPEVAQPVTLVAHEDFE
                     DPAALARWSSLDPEAAVRLTNAPGAVHGGQSALEFTYTPRETVFPMVVGQGMGLAGAR
                     SLSFALMAAERTPLLYGVFEADGSMYSSFLDVPPGVWGEIRVSLGDLQLADESEDENA
                     ALDSDQVDRVFFADLSNLQGEMGRALGWKTSPQTLLIDDVALSAEAVPSRWQAAPDAG
                     AIRFALAPGRIDALAIGGAQLAVVPVDGRPALQITYRIGGWRWAGFVTGLGSLPSADV
                     AGVSFRARVSADARVNVLLEERDRSRYASGADLVAGQGWQNVELPLAAFSLDEFSADE
                     NGRLDPDQLRVLVLITDTFTAAVDDQGKGEILVDSLALHLGHGGR"
     gene            complement(1717..2676)
                     /locus_tag="PLQ54_00400"
     CDS             complement(1717..2676)
                     /locus_tag="PLQ54_00400"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOS91747.1"
                     /translation="MSIEARAAAAGGAALLAALLFTPVCQRAARARSILAKPTGRGVH
                     REPIPYLGGLAILGGVVIGAAFCPYSMKLVGLLLAAVTVTIIGVLDDVRDLSPLVKLL
                     LTMVAAAILIPLDVVVQGINVPGFGFVYLGPWARLLTLLWVVFMVHATNFIDGMDGEA
                     AGISAIAAAAFVVLALLWLRRPQDPDVVADMSMVAVLSSAVVGASLGFLRYNFNPARI
                     FMGDAGALLLGLLLAGLSIVGLFKTVLLCVAPVIILGLQVFDTVWAVVRRTAAGQPAY
                     RPDRGHIHHRLLDAGLTHRQAVLILYAIAAGLAGLAVFVGAPR"
     gene            complement(2738..3982)
                     /gene="glyA"
                     /locus_tag="PLQ54_00405"
     CDS             complement(2738..3982)
                     /gene="glyA"
                     /locus_tag="PLQ54_00405"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOS91748.1"
                     /translation="MSELERVDPEIAAAIRNEERRQCTNLELIASENFVSPAVLAAQG
                     SVLTNKYAEGYPANRYYGGCEFVDVAESLAIERAKRLFGSEHVNVQPHSGTQANMAVY
                     FACLKPGDTILGMNLAHGGHLTHGSPVNFSGFMFRVVAYGVDRETECIDYDEVMRLAL
                     EHKPKLIVAGASAYPREIDFARFRQAADACGAYLMVDMAHIAGLVAAGVHPSPVPHAD
                     FVTTTTHKTLRGPRGGMVLCRSEHAKTIDKMVLPGIQGGPLMHVIAAKAVCLKEAESP
                     AFAEYQRQIARNARALAGALTERGFRLVSGGTDNHLVLVDVSSKGVTGKEAQAFLDAA
                     GITTNKNMIPYDTQKPRVTSGIRLGTPAVTTRGMKEGEMSQIAGLIDAAVAGRDDTGA
                     LARVRSQVVELTTRFPLHVPSA"
     gene            complement(3961..4434)
                     /gene="rpiB"
                     /locus_tag="PLQ54_00410"
     CDS             complement(3961..4434)
                     /gene="rpiB"
                     /locus_tag="PLQ54_00410"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOS91749.1"
                     /translation="MTIAIASDHGGYALRQHLRTLLEAEGQELVDMGCASEEPVDYPP
                     YAFRVAEAVAGGEADVGILICGTGIGMSLCANRVPGVRAAVVGDTYSARMSREHNDAN
                     VLCLGGRTLGPDLAAEIVRVWLATPFSGGERHRRRLAMVAQREGGGTPCCPSSNG"
     gene            complement(4529..6313)
                     /locus_tag="PLQ54_00415"
     CDS             complement(4529..6313)
                     /locus_tag="PLQ54_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS91750.1"
                     /translation="MTDTALNGPSGHDGEPDDRQDGELETAGESEGVPAAGGDAPPGP
                     TPDGTREALRQEYERLRERIRELERQLAEFDYNVPPTTRPALEKTLARLLEKVTMIVR
                     AEKCCVMVYNPETNELEAQEPAIGLTPEQIRSFRVRANEGISGLVFREGQPVIMQEAV
                     TDDRALAELVALLGVRNGATVPLVHERKDDDVVVEEKTIGVLHVFNKRRDRKFMREDL
                     RILRLMARSAAAVISEARLFIELRERADTLEKTYESLPAGIIMVGTDGVVRLMNRAAR
                     QLLTDGSGEDPLGRPYQDVIQSAEMRKLIGNTMVDQQELESELSIGETGRVHRAQTAI
                     VWDGDSFAGVVAICNDVTELRNLEKMKSAFVTTVSHELRTPLTSIQGFTRTLIEDEDD
                     TYPLEARREFLGIIDKECRKLNRLVDDLQKVARIDEGHGLDLTISRVDMAELLQGLVA
                     SYQAYSADHQIELDMTEEFKQAPILSDEDKIDQVVSNLLSNAIKYSPPHTEVRVRGEA
                     RNGGLCVTVADEGRGIPKDQLSRVFDRFYRGEGDHRDTTGGTGLGLYIVKHLVDRLGG
                     AIEVDSEVDRGSTFRVFLPLSPPEPTRK"
     gene            complement(6551..8698)
                     /locus_tag="PLQ54_00420"
     CDS             complement(6551..8698)
                     /locus_tag="PLQ54_00420"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOS91751.1"
                     /translation="MTSPTPRIVRLRQHAIDHGHWTNHEPALYWGRSWQTTAGEIWHI
                     VRRARATAHILENLTPVIDPDELLVGKFAPVTPTEEERAELDQWAAIERLLPVAAGIT
                     AHQAVDIERLLLYGVSGIKELIEGYRRELDVARPEDLEKDAFYRACLIALDGLVALAR
                     RYAERAVGMAEAEEDPARRAELLEIATVCRTVPEYPATTFREALQAAHFLTFALCAGQ
                     RMLLFQLGRPDRYLLPFYRRDIAAGRITPEQAQELLDCAAILLNEYTPKGLAVGYMVG
                     GRDSSGMDVCNELTEMLLEGIGHVRLSYPGVGLCWTPETPPHVMRLAGRLLAEGCTHP
                     AIFQDDVISAGLRAAGVSPGESRLYIHSTCVEITPIGSSNVYVASPYLNVLQLLHDSI
                     GVPPLGAEAPEPTPLYASFDALLHELRRRIGDAVRAAVVDTNAAQLSRRRHGGAPLQS
                     CFVNDCLSRGLDIDHGGARHNWIEPVFVGTANVADSLAAIRQFVFEEKVLSLPELAHA
                     LAHDYEGREDLRAMMIHRAPKYGNDDDSVDSLVARLTSWYSEECRKYRSALGGTYQAG
                     MFCWIQHGELGRVTRASADGRRAGLALADGAGAAQGRDSTGATAAILSATKWDHRPML
                     GGIAVNVRFSPSPDREALGELVRQVCQTYLQRGGAEIQVNVVDTETLREAKRHPEDHR
                     DLVVRIAGYSDYFVSLPDVLQDEVIARTAHELA"
BASE COUNT         1601 a         3128 c         2894 g         1351 t
ORIGIN      
        1 tggcgcctgg ccgcccagtg ccaccggggc ggactccgca cggctcaaga ccttccctcc
       61 gccgatgcgg aagtggcgga cccggcccgg cccgacgagc acgacagagc catcggtggg
      121 cgacgtcctg ttgtagacca cggcgactcc ggcccctgcg ggccaagtag agggagcccc
      181 gatggtgcag cggacgcggt aaggaccggg cacggcctcc gccacgggct cacactggcg
      241 caacgaggcc agggcagagt catccggtgc ggccgcccgg agaccgctgg ctgccagggc
      301 agacacgaca gccacccaca tcacggtgcc ggtggcggtg aatccggtgc gcatggatgg
      361 ttcctcctgc agatcggggt gagcccgatg gcggcaaggg ccacgtcccg ccgtgggcgc
      421 ctaccgcccg ccatgtccca ggtgcagtgc caggctgtca acgagaatct cgcctttgcc
      481 ctggtcatcg acggcggccg tgaatgtatc cgtgatgagc accagcacgc gaagctgatc
      541 cggatcgagc cggccgttct catcagccga gaactcatcc agcgagaagg cggcgagagg
      601 aagctccacg ttctgccacc cctggcccgc caccaagtcg gcccccgagg cgtagcgcga
      661 gcggtcccgc tcctccagca gcacgttcac ccgggcatcc gccgaaacgc gggcccggaa
      721 tgacacaccg gccacatcgg ccgaaggcag gctcccaagg ccggtcacga aacccgccca
      781 acgccagccg ccaatgcgat acgtgatctg cagcgccggc cgaccatcca cgggaaccac
      841 cgcgagctgc gcccctccga tggccaacgc gtcgatgcgt ccgggagcga gcgcgaatcg
      901 gatggcaccg gcatccggag cggcttgcca gcgcgatggg acggcctcgg ccgaaagcgc
      961 cacatcgtcg atgagaagcg tctggggact ggtcttccaa cccagggcgc gccccatctc
     1021 cccctggagg ttcgagaggt cggcgaagaa cacgcggtcc acctggtcgg agtccaaagc
     1081 cgcgttctca tcctcggatt cgtcggcaag ctgcaggtcg cccaggctca cccggatctc
     1141 gccccacacc ccgggcggca catcaaggaa cgacgagtac atgctcccat cggcctcgaa
     1201 cacaccatac agcagcggag tcctctccgc cgccatcagc gcgaacgata ggcttcgggc
     1261 gccggcaaga cccatgccct ggcccacgac catcgggaag accgtctcgc gcggcgtgta
     1321 ggtgaactcg agagcggact ggccaccgtg gacggctccc ggcgcgttgg tgagccggac
     1381 cgctgcctcg gggtcgaggc tactccagcg agcgagtgcc gccggatcct cgaaatcctc
     1441 gtgcgcgacg agggtgacgg gctgagcgac ctccggggca cctggaggct gggcggccgc
     1501 caactgggcg gataaggtgg ccgaggcaaa gacaagcacc gaaacgaaac gtctccgcat
     1561 tcggggacct ccggaacggc gacgcgccgc gcccaccggc tggccgcgcg ggtggcgccc
     1621 atgggatacc aacgcgttag actcaccata gcacccaaag gggcgcggaa ggagcaaaaa
     1681 aagggggcca agacccgccg gagcacccgc ccgtcgctag cgcggcgccc ccacgaacac
     1741 cgcaagccct gccaggcctg cggcgatggc gtagaggata agcacggcct gccgatgggt
     1801 cagcccggca tcgagcagcc ggtggtggat gtgcccgcgg tccggccgat aggcgggctg
     1861 gcccgcggcg gtgcgccgaa cgacggccca gacggtgtcg aagacctgaa ggccgagaat
     1921 aatgaccggc gccacgcaaa gcagcaccgt cttgaacaac cccacaatcg agagaccagc
     1981 caggaggagc cccagcagca gggcgcccgc gtcgcccatg aagatccgag ccgggttgaa
     2041 gttgtagcga aggaacccca acgacgctcc caccacggcc gaactcagaa cggccaccat
     2101 cgacatgtcg gcgaccacat cgggatcctg cggccgccga agccacagga gggcgagcac
     2161 aacgaatgcg gccgcggcaa tggcagagat cccagccgcc tcgccgtcca tcccatcaat
     2221 gaagttggtg gcatggacca tgaacacgac ccacaacaga gtcagcagcc gagcccacgg
     2281 gcccaggtag acaaagccga acccgggaac gttgatgccc tgaacgacga catccagagg
     2341 aatgagaatc gccgccgcca ccatggtcag cagcaacttc accaacggcg agagatcccg
     2401 gacatcgtcg agaacaccga tgatggtgac ggtgacggcc gcgagcagaa ggcccacgag
     2461 cttcatggag taggggcaga aggcggcgcc gatcacgacg ccaccgagaa tcgccaggcc
     2521 ccccaggtag ggaatgggtt cgcggtgaac gccacgccct gtcggtttgg ccagaatgct
     2581 gcgcgcccgt gctgctcgct ggcacaccgg ggtgaacaag agagcggcca gcagagccgc
     2641 cccgcccgcc gcggcagccc gagcctctat ggacaaccga ttgcctccgt agagtccgcc
     2701 ggcgcggggg ctgcggacgt gatgggttcc cagcgcccta ggcgctcgga acatggagag
     2761 ggaagcgagt ggtgagttcc accacctgac tccgcacccg cgccagcgca ccggtatcgt
     2821 cgcggcctgc cacggcagcg tcgatgagcc ccgcgatctg gctcatctcg ccctccttca
     2881 tcccgcgcgt cgttaccgcg ggagtgccga gccgaatccc actggtcacc cgcggcttct
     2941 gggtgtcgta ggggatcatg ttcttgttgg tggtgatgcc ggccgcatcg agaaaggcct
     3001 gggcttcctt gccggtcacg cccttggagc tcacgtcgac cagcaccaga tgattgtccg
     3061 tcccaccgga cacgaggcgg aacccgcgtt cggtgagcgc gccggccagc gctcgcgcat
     3121 tccgggcgat ctgccgctga tactcggcga aggccggcga ctccgcctcc ttcaggcaca
     3181 cggccttggc cgcgatgacg tgcatcagcg ggccgccctg gatgcccggc aggaccatct
     3241 tgtcgatggt cttggcgtgc tcgctccggc agagcaccat gccgccgcgc ggaccacgca
     3301 gggtcttgtg agtcgtggtg gtcacgaagt cggcgtgagg caccggcgag ggatgaacgc
     3361 cggccgcgac cagcccggcg atgtgcgcca tgtccaccat gagataggcc ccgcaggcat
     3421 cggcggcctg gcggaagcgc gcgaagtcga tctcgcgcgg atacgcgctg gcgcccgcca
     3481 cgatgagctt cggcttgtgc tccagggcca ggcgcatcac ctcatcgtag tcgatgcact
     3541 ccgtctcccg gtcgacgccg taagccacga cccggaacat gaagccagag aagttgaccg
     3601 gactgccgtg agtcaggtga ccgccgtggg ccaggttcat gccgaggatg gtgtcgcccg
     3661 gcttgaggca ggcgaagtag acggccatgt tcgcctgggt gccgctgtgg ggctgaacgt
     3721 tcacgtgctc ggacccaaac agcctcttgg cgcgctcgat ggccaatgac tcggccacgt
     3781 cgacgaactc acacccgccg tagtaccgat tggccggata gccctcggcg tacttgttgg
     3841 tgagcacact gccctgggcc gccagaacag ccggactgac gaaattctcg gaagcaatga
     3901 gctccaggtt cgtgcactgg cggcgttcct cgttccgaat cgccgcggcg atctcggggt
     3961 ctacccgttc gagctcggac agcatggagt gcccccaccc tctcgctgcg ccaccatggc
     4021 gaggcggcgg cggtgcctct ctccacccga gaagggggtg gccagccaca cacggacgat
     4081 ctcagcggcc aggtcaggcc ccagagtccg tcctcccaaa cacaacacat tggcgtcatt
     4141 gtgctcgcga gacatgcgcg ccgagtaggt atcgcccacc accgcggcgc ggactccagg
     4201 cacccggttg gcgcacagag acatgccaat acccgtaccg cagatcagga tgcccacgtc
     4261 ggcctcgccc ccggcgacag cctcggcaac ccggaaggcg tagggcgggt agtccacggg
     4321 ctcttcggag gcgcagccca tgtccacgag ctcctgcccc tccgcctcga gcaatgtcct
     4381 caaatgctgg cggagcgcgt acccgccgtg gtctgacgcg atggcgatgg tcacggtgga
     4441 ccctccgttg gctcggtggc cggcgccaag gcgtgccggg gctcgaacac gatgggcccg
     4501 gggcgcgcgg tccacctcga tggcacgcct acttgcgagt tggctccggc ggcgagagcg
     4561 gcagaaacac ccggaacgtg gaacctcggt cgacttcgct atcgacctcg atggcccccc
     4621 cgagccggtc gaccaggtgc ttgacgatat acagccccag ccccgttccg ccggtggtgt
     4681 cccgatggtc tccctcgccc cggtagaagc ggtcgaagac gcggctgagc tggtccttgg
     4741 ggatgcctcg accttcatcc gccacggtca cgcacaggcc cccattccgg gcctcaccgc
     4801 gcacacgaac ctcggtgtgc ggtggcgagt acttgatggc gttgctcagc agattcgaga
     4861 ccacctggtc gatcttgtcc tcatcgctga gaatcggcgc ctgcttgaac tcctcggtca
     4921 tgtccagctc gatctggtga tccgcgctgt acgcctgata cgacgccacc aggccctgca
     4981 ggagttcggc catatccacc cggctgatgg tcaagtcgag cccatggccc tcgtcgatgc
     5041 gggccacctt ctgcaggtcg tcgaccagcc ggttgagttt ccggcactcc ttgtcgatga
     5101 tgccgagaaa ctcgcgccgc gcctccagcg gataggtgtc atcctcatcc tcgatgagtg
     5161 tgcgagtgaa cccctgaatc gacgtgagcg gcgtccgcaa ctcatgcgat acggtggtca
     5221 cgaaggccga cttcatcttc tccagattgc gcagctcggt gacatcgttg caaatcgcga
     5281 ccacgccagc gaagctgtcc ccgtcccaca caatggccgt ctgagcacgg tgcacccggc
     5341 cggtctcgcc gatggagagc tcggactcga gctcttgctg gtccaccatg gtgttgccga
     5401 tcagctttcg catctcggca ctctggatga catcctgata gggccgcccc agcgggtcct
     5461 cgcccgatcc gtccgtcaga agctggcgcg ccgcgcggtt catcaaccgc accacgccat
     5521 cggtgccgac catgatgatg ccagccggca gactctcata cgtcttctcg agagtgtccg
     5581 ctcgctcccg cagctcgatg aacaggcggg cctcggagat caccgcggcc gccgaccggg
     5641 ccatgaggcg gaggatgcgc agatcctcgc gcatgaactt gcggtcacgc cgcttattga
     5701 agacgtggag cacgccgatg gtcttctctt cgacgaccac atcgtcgtcc ttgcgctcgt
     5761 gaaccagcgg caccgtcgcg ccattgcgaa cgcccagcaa ggcaaccagc tcggccaaag
     5821 cccggtcgtc ggtcacggcc tcctgcatga tgaccggctg gccctcacgg aacaccagcc
     5881 cgctaatgcc ctcattcgct cgaacgcgga agctgcggat ctgctccggc gtcagaccaa
     5941 tggccggctc ttgcgcctcc agctcattgg tctcggggtt gtacaccatg acgcagcact
     6001 tctcggcccg cacgatcatg gtgaccttct cgagcagccg tgccagagtc ttctccaggg
     6061 cggggcgagt ggtgggggga acgttgtagt cgaactcggc gagctgtctc tcgagctcac
     6121 ggatacgctc gcgcaggcgc tcatactcct gccgcagagc ctcccgtgtc ccgtcaggcg
     6181 tcggtcccgg cggcgcatct ccaccggcag ccgggacgcc ctcggactcg ccggcggtct
     6241 cgagttcgcc atcctggcgg tcgtcgggct cgccgtcgtg gccagaaggt ccgttcaatg
     6301 cggtatccgt catgagctcg tagtccacac cggaatggcc gggagccctg cgagaaggcc
     6361 agcagtcccg aacccggtct ccttcgcatc cggcagcccc gtgccgggag tcgaagccag
     6421 ggtgattcta gggtatgcgt cgagtgagcg tcaacacgcg agggagcgag ggacggtctc
     6481 cgggggcagg agcccatccg ccagacaccg cggcagaatg cggttcccga cccctgggcc
     6541 gaccgcttcg ctacgccaac tcgtgagccg tgcgggcgat gacctcatcc tggagaacgt
     6601 cgggcaggct gacgaagtag tcgctgtagc ccgcgatgcg cacaaccagg tcacggtggt
     6661 cctccggatg gcgtttggcc tcacggagag tctcggtgtc gaccacattc acctgaatct
     6721 cggcgccgcc ccgctgtagg tacgtctggc atacctgccg gaccaactcg cccagcgcct
     6781 cccgatccgg cgaaggactg aaccgcacat tcaccgcgat gccaccgagc atgggtcgat
     6841 gatcccactt ggtggccgac agaatcgccg ccgtcgcacc ggtcgagtcg cggccctggg
     6901 ccgcccccgc cccgtcggcc agcgccaggc ccgcccgccg gccgtcggcc gaagcgcgcg
     6961 tcaccctgcc cagctcgcca tgctggatcc agcagaacat gcccgcctga tacgtacccc
     7021 ccaaggccga gcggtacttg cggcactcct ccgagtacca tgaagtgagc cgagccacca
     7081 gggaatccac cgagtcgtcg tcgttcccgt acttgggtgc ccgatggatc atcatggcgc
     7141 gaagatcctc gcggccctcg tagtcgtggg ccagcgcatg ggccagctcg gggagcgaaa
     7201 ggaccttctc ttcgaaaacg aactgccgga tcgccgccag cgagtcggcg acgttggccg
     7261 tgcccacaaa caccggctcg atccagttat ggcgggcgcc gccgtggtcg atgtcgagtc
     7321 cgcgggagag gcagtcgttg acgaagcacg actggagcgg ggctccccca tggcgtctcc
     7381 gggacagctg ggctgcgttg gtgtcgacca ccgcggcccg gacggcgtcg ccgatccgcc
     7441 gccgcagctc atgcagcagg gcatcgaaac tggcatacag gggagtgggt tccggcgcct
     7501 cggcccccag aggcggcacc ccgatgctgt cgtgcagcag ctggaggacg ttcagatacg
     7561 ggctcgccac atacacgttg gacgagccaa tgggcgtgat ctcaacacaa gtgctgtgga
     7621 tatacaaccg ggactcgccg ggcgagactc cggctgcccg gaggccagca ctgatcacat
     7681 catcctggaa gatggccgga tgcgtgcacc cctcggccag cagacgaccg gccaggcgca
     7741 tcacgtgggg tggcgtctcg ggcgtccagc agaggcccac cccggggtaa gacaaccgca
     7801 cgtggccgat gccctcgagc agcatctcgg tcagctcgtt gcacacatcc atgcccgacg
     7861 agtcccggcc tccgaccatg tagcccacgg ccaaaccctt gggtgtatac tcgttcaaca
     7921 gtattgcggc gcagtccagg agctcctgag cctgctcggg agtgatccgc ccggcggcga
     7981 tgtcgcggcg gtagaaaggc aacaggtagc gatcggggcg gccgagctga aacaacaaca
     8041 tccgctgccc ggcacacagg gcgaaggtga gaaagtgcgc ggcctgcaga gcctcgcgga
     8101 atgtcgtggc cgggtactcg ggcacggtgc ggcagacggt ggcgatctcc agcagctcgg
     8161 ctcgccgcgc cggatcctcc tcggcctcgg ccatcccaac cgcccgctcc gcgtaccgcc
     8221 gcgccagcgc gactagcccg tcgagggcga tcaaacacgc acggtagaac gcgtccttct
     8281 ccaggtcctc cggccgggcc acatccaact cgcgccggta gccctcgatg agctccttga
     8341 tgcccgacac gccatacaac agcaaccgct cgatgtccac ggcctggtgg gccgtaatgc
     8401 ccgcggccac gggcagcagc cgctcgatcg cggcccactg gtcgagctcc gctcgctcct
     8461 cctcggtggg ggtcaccgga gcgaacttgc ccaccagaag ctcgtcgggg tcgatcacgg
     8521 gcgtcaggtt ctcgaggatg tgcgccgtag cccgggcgcg gcgcacgatg tgccaaatct
     8581 cgccagccgt ggtctgccac gatcgacccc agtagagcgc cggctcgtga ttcgtccagt
     8641 ggccgtgatc gatggcatgc tgcctcaggc ggacaatgcg gggggtgggg gaagtcactg
     8701 ggctgcctct ccatcgggga tctcgatcgc cgtctccaac ccattctacc gcataccgtg
     8761 tgaaccacgg atatggccgt ggtatgaacc acgaggcgcg gccccatggt tcgccgggcc
     8821 gcaccggaga ccgttcgtat cactcgctcc gggtgatgat gtgatctacc tcaacaacgc
     8881 cgcgacgagc taccccaagg cacccggcgt cgtcgacgcc gttcgctcgg cgctgaccga
     8941 cgctcccggc cctcccggcc gcaccgcggc ggag
//
LOCUS       DAOSPL010000011         8950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_7769_length_8950_cov_3.241147, whole genome shotgun sequence.
ACCESSION   DAOSPL010000011 DAOSPL010000000
VERSION     DAOSPL010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8950
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7769_length_8950_cov_3.241147"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="PLQ54_00425"
     CDS             <1..1018
                     /locus_tag="PLQ54_00425"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91752.1"
                     /translation="SHVPLVGQGGLQPPNAMGLYLADAFGNLDLLYRDPGISSMYPIP
                     VRPRPLPPVLSSAVSPAGSEPGRLVLLDVGRGLGGVAAGAVRALRVIGVPPKVQPVMN
                     SPVLGVTGDDPGKFVLGTVPVEADGSAYFSVPSGLSVFFQALDADGLAIQTMRSVTYV
                     QPGQTLACVGCHEARTTAPANAIPLAGTRPPSRLAPGPEGSWPLRYDRLVQPVLDRWC
                     VSCHRPGASDPEAAALDLTAGRSYDALVAYGSPSLADHVRTRYAEGRSEAGAGAARTS
                     ALLGLLRAGGGHRGVVLSGDDLNRLVTWMDTYAQRQGSYSGEQEHLLELLRERSVDLL
                     AASP"
     gene            complement(1015..1722)
                     /locus_tag="PLQ54_00430"
     CDS             complement(1015..1722)
                     /locus_tag="PLQ54_00430"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose-lipid carrier transferase"
                     /protein_id="HOS91753.1"
                     /translation="MLEDLDLSLDVGRKEFEKELAGYQVELVGLQRKIVDQGRRVIIG
                     FEGWDAAGKGGAIKRLILYMDPRGYEVHPIGPPTDMELKHHYLRRFWQRLPARGKLTI
                     FDRTWYGRVLVERVEGFASKAEWKRAYDEINDFERVLVDDGYLLLKFFLHISSKEQLR
                     RFKARQADPLKQWKITEDDWRNRKKRPQYLKAVEDMLARTHTETAPWTLVEADSKRYA
                     RLKVVRTVIGALRAMDD"
     gene            2039..3565
                     /locus_tag="PLQ54_00435"
     CDS             2039..3565
                     /locus_tag="PLQ54_00435"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOS91754.1"
                     /translation="MACAILGAVAVVFGGLLVYSGAVRTSPHIAAGVRISGVDVSGMT
                     REEAAAVIRRDLQPLLNERVELVYGNESAWASMQDLGVAIDADESAAIAYEQGRHAGL
                     IADLRARYQLRRHGLDLPAVVVRDEGRTRKALQRFSADIRREPRNARLELLADGQVRL
                     IPGRSGRVLNLEETVARALTVLSSLHVRRVEMVVEEKAPQVATEDLQDINCVLSSFST
                     PYSAGQVDRSHNLQLACRAIDESLLRPGEVFSYNSAVGNRSKERGFRMAPVYADNDVR
                     PGMGGGVCQPSTTLYNAALLAGLDIVERHPHMMPVHYVQLTRDATVEWAQGLDLRIRN
                     NLTHPVFIKASAAGGRVTMTLLGNQSDKVDVDLIVSNISTTPAEVKEIPDETLEPGTR
                     KVEEPGRAGHRGTLTRIVRRNGVEIKRDGHTDYYRPKTKVVLVGPSADGSVEIPVASA
                     GGSAPGAKPAPAKPAPSKAGGAKPGVSRPSGESSAPASKPSKPQSDGLEELFGSIAGG
                     "
     gene            3677..4264
                     /locus_tag="PLQ54_00440"
     CDS             3677..4264
                     /locus_tag="PLQ54_00440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS91755.1"
                     /translation="MSEWLNIRMNLNSTQGLPPIELAPGYDLITAEEWGFPACLGRWL
                     DLLAVGFSETHAEGGWTEASWRERIVDQPQFDPAGVFFVRHGGQAVATAFAWLDTPDE
                     REWGRVHWVATDPAHRGKGLGRAVTLAVARYFGDRGFPRVFLETQAYRTSAIRVYVKL
                     GFVPFWDNEEHKAQWEHALPSIADLLAERGWTAPE"
     gene            complement(4434..5792)
                     /locus_tag="PLQ54_00445"
     CDS             complement(4434..5792)
                     /locus_tag="PLQ54_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS91756.1"
                     /translation="MARKRVNRRDFIKTSSAATLGAIALPYVVSSSALGKDGRPPASE
                     RIVMGCIGLGGMGTNNMNVFMSKPEVQVVAVCDVHESYNDGNAGRAPAKARAEQHYAD
                     LAGKGTYRGCTAYADFRNLLAREDLDAVSIATPDHWHALTSIAAAEAGLDIYCEKPLA
                     NSVAEGRAICEAVRRNQVILQTGSHERSNPKGRFACELVRNGRIGKLHTIRINLPCSD
                     SHHKDAMSFTTVPPPEPVPAGLDWDMWLGHTPVVPYTPRRCHFWWRFILAYGGGEMTD
                     RGAHIIDIGQLGNGTDDTGPIEIEATGTHSTTGLYDAFFDYHFVNRYANGVELIGEAK
                     DPRGLKFEGDKGSIFIHIHGCDLEADPPSLLDEKIGDDEVQLGRTQGHHQNFLDCVRS
                     RQQPFATAEIGHRTATICHLNNIAMRVGRSLKWDPVTETIQDDDEAAALLAPAMRAPW
                     HL"
     gene            complement(5885..7027)
                     /locus_tag="PLQ54_00450"
     CDS             complement(5885..7027)
                     /locus_tag="PLQ54_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010856048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOS91757.1"
                     /translation="MVGLVLAILALTGTAPRAPLLAFERQRIGTDNYEAASAFDVNND
                     GAIDIVSGAYWYEGPAYQVAHKIAEYQRVSDYYDDFSDYPMDVNGDGWTDIVTGGYFG
                     QTLQWIENPKGAEGLWPLHKVAQVGPIERNLFHDIDGDGFVEVMPTTSPVHIFRLVRD
                     AEGKGTGQFTDFVIAQPGGGGHGMGAGDVNADGRVDLVFSTGWFEAPEKPYEGQWNWH
                     PELTLCSDASVPILVYDVNKDGLPDIIVGHGHDYGLDWWEQGKSAEGDRTWTQHVICS
                     ERSQYHDIQLVDIDKDGEPELLTGKRYRAHAEADPGSLDPVGVYYFEINGGQFERVTL
                     DYGPAGQASGAGIYLWMEDVDGNGWPDVIAPGKDGLYLFRNLGPQP"
     gene            complement(7333..8331)
                     /locus_tag="PLQ54_00455"
     CDS             complement(7333..8331)
                     /locus_tag="PLQ54_00455"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS91758.1"
                     /translation="MAKDKVRVAFVGSGGIAQAHLKELSQMADVELVGFCDVVEDKAQ
                     RAATQYGGHAFTCPRAMFGSVQADAAWICLPPFAHGDAEMSAIEHGVPFIVEKPVGLD
                     LGQCREIAAAAAEKGLITAAAYMNRYRKGIQRARELFATDPPVLSLGGWIGGSPTPVP
                     GTISHWWIQRHKSGGQYVEQVTHTTDLVRYLCGEATHVFAFGAKGFNVGIPDYTIDDA
                     AAVSILLKNGGVANLCSCCASGASGGVWLDVFAKQTCARFSGWEHTAKITVAGQETEE
                     IAGEGDIFRIEDAAFIQAVKSGDASGVLSTYADGVESLALGLAANRAIETGQVQEL"
     gene            8528..>8950
                     /locus_tag="PLQ54_00460"
     CDS             8528..>8950
                     /locus_tag="PLQ54_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91759.1"
                     /translation="MAGSRMTRRDACKTLAAAAAVTLGAEAARAQGDPVTVEPAETDE
                     LLANPHMGWQTFHRFADDDEALAGLPSSSAYFRFYWSQIEKTKGQVNLDFLDSLLERA
                     DAAGQKLAFRVMCAGTDDAYMHVPPWLKDMGCPGFEFHY"
BASE COUNT         1323 a         2805 c         3193 g         1629 t
ORIGIN      
        1 gagccacgtg cccctggtgg gtcaaggtgg tcttcagcca ccgaatgcca tgggactcta
       61 tctggcggat gcgtttggca acctggatct gctctatcgc gaccccggga tcagcagcat
      121 gtacccgatt ccggttcggc ctcggcccct cccgccagtg ctgagcagcg cggtgtcgcc
      181 cgccgggtcc gagcccggtc ggttggtgct tctcgacgtg ggccgggggc tggggggggt
      241 ggcggccggt gcggttcgtg ccctgcgggt catcggcgtg ccaccgaagg tccagccggt
      301 gatgaacagc cccgtgctgg gcgtgacggg tgatgacccg ggcaagttcg tgctgggcac
      361 ggtgccggtc gaggcagatg gctcggctta cttcagtgtg ccctcgggcc tctcggtgtt
      421 cttccaggca ctcgacgccg atggccttgc catccagacc atgcggtcgg tgacctacgt
      481 acagccgggg cagacgttgg cgtgtgtcgg atgccacgag gcgcggacga cggccccggc
      541 caatgcgatc ccgctggcgg gcacccggcc gccctcgcgg ttggctcccg ggccggaggg
      601 ctcgtggccg ctgcgctacg atcggctcgt ccagcccgtt ctcgaccgct ggtgcgtgtc
      661 gtgccaccgt cctggagcca gtgacccgga ggccgcggct ctggacctca ccgcgggccg
      721 gtcatacgat gcgctcgtcg cctacggctc accgagcttg gcggaccatg tgcggacgcg
      781 gtatgccgag ggccgctcgg aagccggggc cggggcggcg cggacgagtg ccctcctggg
      841 gctgctgcgc gccggcggag gccatcgcgg agtggtgctg tctggggatg atctgaaccg
      901 gctcgtaacc tggatggaca cctatgccca acgccagggc tcgtacagcg gcgagcagga
      961 gcacctgctc gagttactgc gagagcgttc ggtggatttg ctggcggcga gtccttagtc
     1021 atccatggcc cgcagggcac cgatcaccgt gcgaacgact ttgaggcggg cgtagcgctt
     1081 gctgtcggcc tcgaccaagg tccacggcgc cgtttcggtg tgggtgcgtg cgagcatgtc
     1141 ctcgacggcc ttcaggtatt ggggacgctt cttgcggttt cgccagtcgt cttcggtgat
     1201 cttccactgc ttgaggggat cggcctggcg ggctttgaag cgccggagct gctccttgct
     1261 cgagatgtgc aggaagaact tgagcaacag gtatccgtcg tcgaccaaca cacgctcgaa
     1321 gtcgttgatt tcgtcgtagg cccgtttcca ctcggctttc gaggcgaagc cttcgacgcg
     1381 ctccacgagt acgcggccgt accaggttcg gtcgaagatg gtgagtttgc ctcgagcggg
     1441 cagtcgttgc cagaaccgac ggagatagtg gtgcttgagc tccatgtcgg tcggcgggcc
     1501 gatcggatgg acctcatagc cgcgagggtc catgtagagg atcagccgct tgatggcccc
     1561 gcccttaccg gccgcgtccc agccctcgaa cccgatgatc acccgccgtc cctggtcgac
     1621 gatctttcgt tgcagcccga cgagctcgac ctgatagccc gcgagttcct tctcgaactc
     1681 cttgcgaccg acgtccagtg agaggtccag gtcctcgagc aatcgagttg ccccctgatt
     1741 gccagcggga gcgaaccgcg cgcccgcggc ggatgtcata cacccgtcag attccgcggg
     1801 atggtcggtc ctcccttgcg gcgatgtcgc acgggccttg acaggggtgt cgcgggtcga
     1861 tggcgttttg gcggacgcca cgaaaaggca atactatcag catagggtcg gtgtaagggc
     1921 gtgtgaggct ggcccgcaga tggagtgtat cgtagttctc atgtcagaag gtacccggag
     1981 cagagcattg ggtcgggccg tgtccgggcc gtcgaagtgg cggcgtcagg cgtccacgat
     2041 ggcttgcgcc atcctgggtg ccgtcgcggt ggtgttcggc ggcctgctcg tctactcggg
     2101 cgcggtgagg acctcgccgc acattgcggc gggggtgagg atctctggtg tggacgtatc
     2161 gggcatgacc cgcgaggaag ccgcggcggt gatccggcgt gacctgcagc cgctgcttaa
     2221 cgagcgggtc gaactcgtgt atggcaacga gtcggcgtgg gcttcgatgc aggacttggg
     2281 cgtggccatc gatgcggatg agtcggcggc catcgcctac gagcaggggc gacacgcggg
     2341 gctcattgcc gatctgcgcg cccgctacca gctccgtcgg catggtctgg acctgccggc
     2401 ggtggtggtg cgagacgagg gccgcacgcg gaaggctctc caacgcttct cggcggatat
     2461 ccgccgcgag ccgaggaacg cccggctgga gctcctggcg gacggccagg tccgcctgat
     2521 cccggggcgt tcgggacgag tgctgaacct cgaggagacg gtggcgcgag ccctgaccgt
     2581 gctctcgtcg ctgcatgtcc gccgcgttga gatggtggtc gaggagaagg caccgcaggt
     2641 ggcgaccgag gatctgcagg acatcaactg tgtgctctca tcgttcagca cgccgtactc
     2701 ggcagggcag gtggaccgct cgcacaacct gcaattggcg tgccgggcca tcgatgagtc
     2761 gctgctgcgg cctggcgagg tgttctccta caacagcgcc gtcgggaacc gctccaagga
     2821 gcgcgggttc cgtatggccc cggtgtatgc cgacaacgac gtgcggccgg ggatgggagg
     2881 cggggtctgc caaccgtcga cgacgctgta taacgccgcc ctgctggcgg ggctcgacat
     2941 cgtcgagcga catcctcata tgatgccggt ccactacgtc cagttgacgc gggacgccac
     3001 ggtggagtgg gcgcagggcc tggacttgcg gatccgcaat aacctgactc atcctgtctt
     3061 catcaaggct tcggcggctg gcgggcgggt cacgatgacg ctgctcggca accagtcgga
     3121 caaggtggac gtggacctga ttgtctccaa catcagcacc acgccggccg aggtgaagga
     3181 gattccggat gagaccctcg agccgggcac ccggaaggtc gaagagccgg ggcgtgcggg
     3241 ccaccgcggg acactcacgc gcatcgttcg gcgcaacggg gtcgagatca agcgggatgg
     3301 tcacaccgat tactaccgcc cgaagacgaa ggtggtcctg gtggggccga gcgcggatgg
     3361 ctcggtggag atacccgtgg cctcggccgg cgggagtgcg ccgggcgcca agccggcgcc
     3421 cgccaagcct gccccgagta aggcgggcgg tgccaagcca ggtgtctcgc ggccttcggg
     3481 cgagtcttcg gcgcccgcca gcaagccttc caagcctcag agtgatgggc tcgaggagct
     3541 gtttggcagt atcgcgggcg ggtaggccgg cttcggcccg gctgcggcgc gtgccctctt
     3601 cgagcgctcc cgagcgtggg ctatggtgca gtgttgtctg ccgcacgcgg cgaagcccgt
     3661 gtcaggagga tccccggtgt ccgagtggct caacatccgc atgaacctga atagcacgca
     3721 gggcctgccg cccatcgagc tggccccagg ctatgatctg atcacggcgg aggagtgggg
     3781 cttccccgcg tgcctcggcc ggtggctcga tttgctggcc gtggggtttt cggaaacgca
     3841 cgcggagggc gggtggactg aggcgagctg gcgcgagcgg atcgtggatc agccgcagtt
     3901 tgacccggcg ggcgtgttct tcgtccggca tggcggtcag gccgtggcca ccgcgtttgc
     3961 ctggctggac acgccggatg agcgcgagtg gggtcgggtc cactgggtgg ccaccgaccc
     4021 ggctcaccgg gggaagggcc tgggccgggc tgtaacgcta gccgtcgccc ggtacttcgg
     4081 tgatcgcggt ttccctcggg tcttcctgga gacgcaggcg taccgcacgt ctgcgattcg
     4141 cgtgtacgtg aagctgggct tcgtgccctt ctgggacaac gaggagcaca aggcccagtg
     4201 ggagcacgcg ctgccgagca tcgccgacct tctcgcggaa cgcggctgga ccgcgccgga
     4261 atagggcgcg gcaagggcag ccgaatgggc tccgcacccc gtccgcaccc gcctggcggc
     4321 gggtcgagga cctcgggtgg ggagtgctgc acccacccga ggctccatac cccgagagtc
     4381 tttggcgacg atgcgcctgg tccgagcgac cgcccggtga gttggcctgc cggctacagg
     4441 tgccagggcg cgcgcatggc cggtgccagt agggccgctg cttcgtcgtc atcctgaatg
     4501 gtctcggtca ccgggtccca cttcaggctg cggccgaccc gcatggcgat gttgttgagg
     4561 tggcagatgg ttgcagtgcg gtggccgatc tcagccgtgg cgaagggctg ctgccgggat
     4621 ctcacgcagt cgaggaagtt ctgatggtgc ccctgggtgc ggcccagctg cacctcatcg
     4681 tcgccgatct tctcgtccag cagcgacggg ggatctgcct cgaggtcgca tccgtggatg
     4741 tggatgaaga tcgatccctt gtcgccctcg aacttgaggc cccgggggtc cttggcctcc
     4801 ccgatgagct ccacaccgtt cgcgtagcgg ttgacgaagt ggtagtcgaa gaaggcatcg
     4861 tagagccctg tggtcgagtg ggtcccggtg gcctcgatct cgatgggtcc ggtgtcgtcg
     4921 gtgccgttgc cgagctggcc gatgtcgatg atgtgggcgc cgcggtcggt catctcgccg
     4981 ccgccatagg ccaggatgaa ccgccaccag aagtggcagc ggcgtggggt gtagggtacc
     5041 acgggggtgt ggccgagcca catatcccag tcgaggccgg cggggacggg ctcgggcggg
     5101 gggacggttg tgaacgacat ggcgtctttg tggtggctgt cggagcacgg caggttgatt
     5161 cggatggtgt ggagttttcc gatgcggccg ttgcgcacca gttcgcaggc gaagcggccc
     5221 ttgggattcg atcgttcgtg gctgccggtc tgcaggatga cctggttgcg ccggacggcc
     5281 tcacagatgg ctctaccctc ggccacggag ttcgcgagtg gtttctcgca gtagatgtcc
     5341 aggcctgctt cggccgcggc gatggacgtg agcgcgtgcc agtggtcagg tgtggcgatc
     5401 gagacggcat cgaggtcctc acgcgccaga aggttccgga agtcggcata ggccgtgcat
     5461 ccccggtagg tgcccttgcc ggccaggtcg gcgtagtgct gctcggcccg cgccttggcg
     5521 ggtgcgcgac ccgcgttccc gtcgttgtag ctttcgtgca cgtcgcacac cgcgacgacc
     5581 tgaacctcgg gcttggacat gaagacgttc atgttgttgg tgcccatgcc gcccaggccg
     5641 atgcagccca tgacgatgcg ttcgctggcg ggcgggcggc catctttgcc caaggcggat
     5701 gacgagacga cataggggag tgcgatggcg cccagcgttg ccgccgagct tgtcttgatg
     5761 aagtccctcc ggttgaccct cttgcgtgcc atcagtgccg gacctcctcg cggtatgggc
     5821 ggctccacgc ggtgatgtgg gcgcgttggg cggagcagcg cccgatgttt gggttgtgcc
     5881 gtggctatgg ttgcggtccc aggttacgga acaggtacag accgtccttg cccggggcga
     5941 tgacgtcggg ccagccgttg ccgtcgacgt cttccatcca caggtagata ccggcgccac
     6001 tggcctggcc ggcggggccg tagtccagag tgacgcgctc aaactgcccc ccgttgatct
     6061 cgaagtagta cacccccaca gggtcgagag acccggggtc tgcctccgcg tgggcgcggt
     6121 agcgcttgcc ggtgagcagc tcgggctcgc cgtccttgtc gatgtcgact agctggatgt
     6181 cgtggtattg cgagcgttcc gagcagatga cgtgctgggt ccaggtgcgg tcgccctcgg
     6241 cactcttgcc ctgctcccac caatcgagac catagtcgtg accatgaccg acgatgatgt
     6301 ccgggaggcc gtccttgttc acgtcgtata ccaggatcgg aacactggca tcggagcaga
     6361 gggtcagctc aggatgccag ttccactggc cctcataggg cttctcaggc gcttcgaacc
     6421 agccggtcga gaagaccaga tcgacgcgcc cgtcggcgtt gacgtcgccg gcgcccatgc
     6481 cgtggccgcc gccaccgggc tgcgcgatca cgaagtcggt gaactgcccg gtgcccttgc
     6541 cctcggcatc gcggaccagc cggaagatgt gcacgggtga ggtagtcggc atgacctcaa
     6601 cgaagccgtc gccgtcgatg tcatggaaga ggttccgctc gatggggccg acttgggcga
     6661 ccttgtgcag tggccagagc ccttcggcgc ccttggggtt ctcgatccac tggagcgtct
     6721 gcccgaagta gccgccggtc acgatatcgg tccagccatc gccattgacg tccatggggt
     6781 agtcggagaa gtcgtcatag tagtcgctga cgcgctggta ctcggcgatc ttgtgggcga
     6841 cctggtacgc ggggccttcg taccagtagg cgcctgagac gatgtcgatg gcgccatcgt
     6901 tgttgacgtc gaaggccgac gcggcctcgt agttgtcggt tccgatacgc tgtcgctcga
     6961 aggccaacag aggcgcccgg ggggcggtgc ccgtgagcgc cagaatcgcc agcaccaagc
     7021 ctaccatgtg tcccatcctc atccggcgga ataggaggga gttcgagcgg cgggagccgg
     7081 agccctctgc ccggtggggc tcgcgcgctg tatcggtccc ccgcgaggag tttccgcggc
     7141 gccccgcggc ggataatcgc atcgccggct tgggggggat gggcggctgc cctccctgcc
     7201 gctccggtcc ccacggccca cggcactcgc tcagccggct ggcgccgtcc cttgcgcctt
     7261 acatcagggc ggcgagaggc ccggcgtggg ccgggcctct tctggttcgg gccgcctgga
     7321 cgtctcgcgg ggctacagct cctgcacctg gccggtctcg atggcgcggt tggctgccag
     7381 accgagggcg agggactcga cgccgtcggc gtaggtggac aacacacctg aggcatcgcc
     7441 cgacttcacg gcctggatga aagcggcatc ctcgatgcgg aagatgtcgc cctcgccggc
     7501 aatctcctca gtctcctgac ccgctacggt gatcttggcg gtgtgctccc agcccgagaa
     7561 tcgggcacag gtctgcttgg cgaagacgtc cagccacacg ccaccgctgg cgccgctggc
     7621 gcagcatgag cacagattgg cgacgccacc gttcttgagc agaatggaga cggccgcggc
     7681 gtcgtcgatg gtgtagtcgg ggatacccac gttgaagcct ttggcgccga aggcgaagac
     7741 gtgggtcgct tcgccgcata gatagcggac caggtccgtg gtgtgcgtca cctgctcgac
     7801 gtactgccct ccgctcttgt gcctctggat ccaccagtga gagatggttc ccggcactgg
     7861 cgtgggcgat ccgccgatcc agcctcccag cgagaggacg ggcgggtcgg tcgcgaagag
     7921 ctcccgggcc cgctggatgc ccttacggta gcggttcatg taggctgcgg cggtgatgag
     7981 acctttttcg gccgcggcgg ccgcgatctc gcggcactgg cccaggtcga gcccgaccgg
     8041 cttctcgacg atgaacggca cgccgtgctc gatggcggac atctcggcgt cgccatgggc
     8101 gaagggcggg aggcagatcc acgccgcatc ggcctgcacg gagccgaaca tggctcttgg
     8161 gcaggtgaag gcgtgtccgc cgtactgggt cgcggccctc tgggccttgt cctccaccac
     8221 atcgcaaaag cccacgagtt ccacgtcggc catctgcgag agttccttca ggtgtgcttg
     8281 ggcgattccg cccgatccca cgaatgcgac tctgacctta tccttcgcca accggggtct
     8341 ctccctatgt gtgcggatcg agtctgggcc cgcggccgcg gagcggcctt ggggtcgggc
     8401 ggtagttcgg accgcgggtg gtccatgcct tcgccgacgc gcgttcgcag gccgcgaacg
     8461 ttcgctcacg gaaacccacg gagctactga catccgcagt tggtgccatc cgggagggct
     8521 gttggccatg gctggttcga gaatgacccg cagggatgcg tgcaagaccc tggccgcggc
     8581 ggccgccgtg actctcgggg ccgaggccgc acgggctcaa ggggaccccg tcacggtcga
     8641 gccggctgag accgacgagc tgctcgccaa cccgcacatg ggctggcaga cctttcaccg
     8701 gttcgccgac gacgacgagg cgttggcggg gctgcccagc tcgtcggcct acttccgttt
     8761 ctactggagt cagatcgaga agaccaaggg ccaggttaac ctggacttcc tggactcgct
     8821 cctcgagcgt gcagacgccg ccggccagaa gctggccttc cgcgtgatgt gtgccggcac
     8881 tgacgatgcg tacatgcacg tgcccccgtg gctcaaggac atgggctgcc ccggtttcga
     8941 gttccactat
//
LOCUS       DAOSPL010000012         8725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_8038_length_8725_cov_5.404614, whole genome shotgun sequence.
ACCESSION   DAOSPL010000012 DAOSPL010000000
VERSION     DAOSPL010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8725
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8038_length_8725_cov_5.404614"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="PLQ54_00465"
     CDS             <1..1439
                     /locus_tag="PLQ54_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HOS91760.1"
                     /translation="RGEMRDPMTELTNHLDELAQNIQKEIAAAQKSAEAKAASVTRMA
                     TIVTVVAAVIGLVIGLVISFALSRGIGAVVGGMQELAAGNLTLEVPVTSQDEIGALAR
                     AYNASREQLRELISSVVNTAGAVATASDEVAGAANESAKGAQEISQTVEQMARGAEDQ
                     TKAVNLMREQMQGLDSAISQVASGAQDQVRLVEHTMTSVHRMSQAVEQVATLAPTVTQ
                     VAVEASENAQQGAKAVSEGVAAMHRIRERAAESMRLIEDLNKQSEAIGEIVSVIEDVA
                     EQTNLLALNAAIEAARAGEHGKGFAVVADEVRKLAERSSTSTGEITSLIKEIQAGIQA
                     VVRAQEQSSQETEQGAALVDQAGQSLESILSSVEQVVTMMTQQVSTAASEMTNNVSMV
                     VQAMEQISATTEENSAAAEEMTATSGEVARSIDSVAAVAEESAAAAEEASASSEEQSA
                     SAEEMAATSEELSASAQSLQAMVAQFRY"
     gene            complement(1790..2296)
                     /locus_tag="PLQ54_00470"
     CDS             complement(1790..2296)
                     /locus_tag="PLQ54_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012857009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOS91761.1"
                     /translation="MYEKSIELMNKAVSEELQAVHQYMYFHFHADDQGYDPLSALFRR
                     IAIEEMLHIERLAERILYLKGEVEMVPAAPVSKITEPLAMLEMALRLEQEAIRSYNLS
                     ANECGAQADSATKRLFEDLVMEEEAHFDQFDTQLEMVRKYGEQFLALQGIERSKTLAA
                     AVPPPTAG"
     gene            complement(2759..3070)
                     /locus_tag="PLQ54_00475"
     CDS             complement(2759..3070)
                     /locus_tag="PLQ54_00475"
                     /inference="COORDINATES: protein motif:HMM:NF027117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOS91762.1"
                     /translation="MRERRYIVVPSRLAEKDLGRLSAPDADKAMRALLALEDEPLRGK
                     PLKGALAPARRIDFGLSGGWYRAAYVLDAETDSIVVFMVGPRGGFYEEATRRRQALRR
                     G"
     gene            complement(3063..3302)
                     /locus_tag="PLQ54_00480"
     CDS             complement(3063..3302)
                     /locus_tag="PLQ54_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOS91763.1"
                     /translation="MTSTVDSKGRVTIPAALRKALKIREGDTVFIDAQGETLRMAKAE
                     SPFDALAAAAIAEHRAGKTRSLREYAKSRGIDLDA"
     gene            complement(3506..3829)
                     /locus_tag="PLQ54_00485"
     CDS             complement(3506..3829)
                     /locus_tag="PLQ54_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91764.1"
                     /translation="MNEDRCAVRLHRGAEKDLAGLSPPDAQRAVDEILRLENNPLLGR
                     PLQGALKPAKRITFGLSGGWYRAAYLYLSNERVCIVFMIGPRGGFYERAQSRFRALKK
                     QYGIR"
     gene            complement(3822..4127)
                     /locus_tag="PLQ54_00490"
     CDS             complement(3822..4127)
                     /locus_tag="PLQ54_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOS91765.1"
                     /translation="MTTTIDAKGRVTIPAEVRRALGMREGDTLFLSAEDGIVRLAKAQ
                     NPFDPSEPITDENIAERLASVTNPYDALAMEAEAEFRAGRTRTIREYAKARGLKLDE"
     gene            4464..5750
                     /locus_tag="PLQ54_00495"
     CDS             4464..5750
                     /locus_tag="PLQ54_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOS91766.1"
                     /translation="MELPEKPAVIGLGYVGLPLAVALGRAQAGTIGFDVSPGRVEELR
                     RGHDSTGETLPDALAASDIHFTCDPADLAAATFYIVAVPTPVTPQRQPDLSMVEGASR
                     TVGRVLKPGDVVVYESTVYPGVTEDCCAPVLERESGLAAGRDFGLGYSPERINPGDKQ
                     HTLESIVKVVAGDTPETLERVAALYESIVPAGVFRASSIRVAEAAKVIENTQRDVNIA
                     LMNELAMIFDRMGIRTTEVLEAARTKWNFLPFRPGLVGGHCIGVDPYYLASAAETVGI
                     HPDMILSGRRTNSGMGEFVARKTVKLLHQAGHPVAGSRVLVMGMTFKENIPDYRNSQA
                     PQIIGELREYGVECFAHDPMVDAASFQRSTGIELVGDGDLAEFDGIVLAVPHRAYIEL
                     GAVGLGRLLRRPGVLVDVTSALKPAEIPAGIRYWSL"
     gene            5764..6726
                     /locus_tag="PLQ54_00500"
     CDS             5764..6726
                     /locus_tag="PLQ54_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOS91767.1"
                     /translation="MTRVLITGGAGFIGSHLVDACLAKGFEVRVFDNFATGKRENLAH
                     VAGDVELVEADLRDLDAVRKAMRGVELVSHQGALGSVPRSVADPLTTNAVNVDGTLHV
                     LVAARDAGVRRLVFASSSSVYGDTVELPKREEMPPRPKSPYACSKLAAEEYCKTFHKV
                     YGLGTIALRYFNVFGPRQDPTSQYAAVIPKFVTALCTGARPVVYGDGTQSRDFTFIRN
                     VAEANLLALNAPEEALGQAYNIALNDQVTLLQILDNLGRIIGVTPNPEFAEARAGDVH
                     DSRAAIDLARERLGYQPRVGCQEGLEQTVAWYREGGTGRLDWGT"
     gene            complement(6980..7993)
                     /locus_tag="PLQ54_00505"
     CDS             complement(6980..7993)
                     /locus_tag="PLQ54_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOS91768.1"
                     /translation="MDLTLREIDPSEAGILDAFAADQPGTIAMQMAAWAQVIEAEPGV
                     RARCVLLSDDAGPVASAVLSLRPYGPGFRGLICYGGPILRDPNSPELWRGVLTALARY
                     GRRQRGVFLRIWPWVPFAQSAMPGLAQCDMRPFERVIAYGYTRYVDLSLSPEELLAQM
                     HSSGRRDARACERKGVRIEHGTSEELLGLFWQLYTQSNELKGLRSRPLPTLRAIMAHD
                     LGSVHVALWEGQPTQGILLVHHPTLPTTYYLCAGTNREITGIPAGFLVHWKAMQWARE
                     RGFRIYDLGGIGDPADPSRGSLASVDVFKRRLGGTDVRIFGEHQKALVPAALLRLAQR
                     LRG"
     gene            complement(8104..>8725)
                     /locus_tag="PLQ54_00510"
     CDS             complement(8104..>8725)
                     /locus_tag="PLQ54_00510"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOS91769.1"
                     /translation="RLYILPDPQPNAFATGRSPAHAAVALNQGLLDRLGPREIEGVLA
                     HEIAHIKHRDTLTMTLVATLAGAVMTLVQLGYFASMFGGMARDDEEGGGGPVVALLML
                     LFAPLAATLIQLGISRTREYEADASAARLTGNPEGLARALLKLEQSAEQIPSHATPAT
                     AHLFIVNPLSGAGGMMLSLFQTHPPIQQRVERLLRQARQPAPAVVR"
BASE COUNT         1537 a         2784 c         2871 g         1533 t
ORIGIN      
        1 tgcgcggcga gatgcgcgac cccatgaccg agctcacgaa ccatctggac gagctggcac
       61 agaacatcca gaaggagatc gcggccgcgc agaagagcgc cgaggcgaaa gccgcgagtg
      121 tcacccgcat ggcgaccatc gtgacggtcg tcgcggccgt tatcggcctg gtcatcggcc
      181 tagtcatcag cttcgctctc tcgcggggta tcggcgcggt cgtgggtggc atgcaagaac
      241 tcgccgcggg caacctgacc ctcgaggtcc ccgtgacgtc gcaggatgag atcggcgcac
      301 tggcacgcgc ctataacgcc agccgcgagc agctgcgcga gctgatctcc tcggtcgtga
      361 acacggctgg cgccgtggcc acggccagcg acgaggtcgc cggtgcggcc aacgagagcg
      421 ccaagggtgc tcaggagatc tcccagaccg tcgagcagat ggcccggggc gccgaggatc
      481 agaccaaggc cgtgaacctc atgcgcgagc agatgcaggg cctggactcg gctatctctc
      541 aggtggcctc cggcgcgcag gatcaggtgc ggctggtcga gcacaccatg accagtgtgc
      601 accgcatgtc ccaggccgtc gagcaggtgg ccaccctggc acccaccgtc acccaggtgg
      661 ccgtggaagc ttcggaaaac gcccaacagg gcgccaaggc ggtcagcgag ggtgtcgcgg
      721 ccatgcaccg catccgcgag cgggcggccg agtccatgcg cctgatcgag gacctgaaca
      781 agcagtcgga ggccatcggc gagatcgtat cggtcatcga agacgtggcc gaacagacca
      841 atctgctcgc tctgaacgcc gccatcgagg cggcccgtgc cggcgagcac ggcaagggct
      901 tcgccgtggt ggccgacgag gtgcggaagc tggccgagcg gtcctcgacc tctaccggcg
      961 agatcacctc gctcatcaag gagatccagg ccggcatcca ggccgtggtg cgcgcccagg
     1021 aacagagcag ccaggagacc gagcaaggag cggccctggt cgaccaggcc ggtcagtctc
     1081 tcgagagcat cctgagttcc gtcgagcagg tggtcacgat gatgacccag caggtctcga
     1141 cggcggcgtc ggagatgacc aacaacgtat ccatggtggt ccaggccatg gagcagatct
     1201 cggcgacgac cgaggagaac agcgccgcgg ccgaggagat gacggccact tcgggcgagg
     1261 tggcgcggtc catcgactcc gtggccgcgg tggccgagga gagcgcggcg gcagcggaag
     1321 aggcctccgc ttcttcggaa gagcagtcgg cctcggccga ggaaatggct gccacgtccg
     1381 aagagctgtc ggcttcggct cagtcgctcc aggccatggt ggctcagttc cgctattagc
     1441 ttcgcgaggg gaatggggcc gaggaagcgt cggccacaga tcatatcggt gtgaaaccgg
     1501 gggcgggcca ttcaacccgc ccccttctcc atctcatcgg cacgctcggg atcattcatg
     1561 gggtccgagt ccgtatgaac cgcgaactct ttgcggatgt tcctctcacg acaaaggaac
     1621 atctggtata ttctggcgtg tgtcttggga cgcgggttgt ggtgtgtgga tcttctcgct
     1681 gaccgaacat cgggcctagt ttcgatgact cccgcgctgc gctcccagcg cttctccaga
     1741 ggcctcaccg gtctcccccg gtgaggcctc tggctttggt gcgagccggt tagccggcgg
     1801 tgggaggggg caccgctgcc gccagcgttt tgctgcgctc gatgccctgc agggccagga
     1861 actgctcccc gtacttgcgg accatctcca gctgggtatc gaactgatcg aagtgcgcct
     1921 cttcctccat gaccaggtcc tcaaagaggc gcttggtggc ggagtcggcc tgggcgccac
     1981 actcattggc cgagaggttg tagctccgaa tggcctcctg ctcgagccgg agcgccatct
     2041 cgagcatggc cagcggctcg gtgatcttcg agaccggagc cgcggggacc atctcgacct
     2101 cgcctttgag gtacagaatc cgctcggcca gccgctcgat gtggagcatt tcctcgatgg
     2161 caatccggcg gaacagagcg gagagcgggt catagccctg gtcgtcggcg tggaagtgga
     2221 agtacatgta ctgatgcacg gcctgaagct cctcggagac ggccttgttc atgagctcga
     2281 tgctcttctc atacatgcgg ggcatccctc atcctcaaat agagtgtggt ctgtttctcc
     2341 aatggggaat gagccttcgt agttcccgcg cgcgaatcct ggacgggtgg gggcttccga
     2401 agacctcccg caccgagagg aggggccgaa tggaaggggc ccgcgaagcg gtaagggcgc
     2461 catccggccc gccctcgaga acgaaatggc cctggccatc ctgtctgagg cctctgatgt
     2521 gggtctccag caacaacggc gaagagcagg ggaacaggat agccaggatg ggcaggataa
     2581 cccccaacac cactggcctt cccaggtagg acagggtctc atcctgtcta tcctggtcat
     2641 cctgtttgag gtccttgacg tgggtctccc acagcaacgg cgaagagcgg ataaacatga
     2701 tgggcccgac acaaccggcc cgctagccga cgcgaggcgg tctgttgcgt gagtctcgtt
     2761 aaccgcgccg cagcgcctgg cgacgtcgcg tcgcctcttc gtagaagccc ccgcgtggtc
     2821 cgaccatgaa cacaacgata gagtccgtct ctgcatcgag aacataggca gcccggtacc
     2881 acccgccgga cagaccgaaa tcaatcctcc gtgcaggagc gagtgctccc ttcaggggct
     2941 tgccccgcaa gggctcatcc tccagagcca ggagagcccg catcgctttg tcggcgtctg
     3001 gagcgctcag ccggcccagg tccttttccg cgagccgaga cgggaccacg atgtagcggc
     3061 gttcacgcat cgaggtcgat acctcgggat ttggcgtatt cccgcaggga acgagtcttg
     3121 ccagcgcggt gctccgcgat ggccgctgcg gccaaagcgt cgaaggggct ctcggccttc
     3181 gccatgcgca gagtctcccc ctgagcgtct atgaataccg tgtcgccctc gcggatcttg
     3241 agggccttcc gcaaagcggc cggaatcgtg acccgccctt tggagtcaac tgtggacgtc
     3301 aaggcgctct ccccctaggt gggactttcc cacatcattg tagcgcatca atgcgcccgg
     3361 ccaccatcgc cccggtgaat ggtagagaac aagatgggca ggatagcccc cagtacaacc
     3421 gggcttctcg ggcaggacgg ggtcccatcc tgtctatcct ggtcatcctg tttgagcctc
     3481 tctggggtgg atcgccatgg ccaggctagc ggatgccgta ctgcttcttg agtgctcgga
     3541 agcggctctg ggctctctcg tagaaccctc cccgaggccc gatcatgaac acgatgcaga
     3601 cccgctcatt cgacaggtac aggtatgcgg cgcgatacca tcccccggat aggccgaagg
     3661 tgatcctctt ggcgggcttc aaggctccct ggagaggccg gccgaggagt gggttgttct
     3721 ccagtctcag aatctcgtcc acggctctct gggcatccgg aggactcagg ccggccaggt
     3781 ccttctccgc gcctcgatgc agccgaaccg cacaccgatc ctcattcatc gagtttcagg
     3841 cctcgagcct tggcatactc ccgaatcgtg cgagtacgcc cagctcgaaa ctcggcttcc
     3901 gcctccatcg ccagcgcgtc gtaagggttc gtgacgctgg caagccgttc agcgatgttc
     3961 tcgtcggtga tgggttcgct ggggtcgaag ggattctgag cctttgccag cctcacgatg
     4021 ccatcctccg cactgagaaa gagcgtatcg ccctcgcgca tgcccagggc gcggcgaacc
     4081 tcagcaggga tggtgacacg gcccttggcg tcgattgtcg tggtcatcgc agttcccctc
     4141 tgtgtgggat gatcccacac cattgtagcg cgtcgatgcg cccggccacc atcgccccgg
     4201 cgaatggcag agaacaagat gggcaggata gcccccatgg aaccggccct ctcaggcagg
     4261 acggggcttc atcctgtcca tcctggttat cctgtttggg gctcctgacg tggatttccc
     4321 acagctatac tgaacataat gccaattata cgacctccaa tggccctgcc ccgctcgcgt
     4381 gcggccgaac cgccaccatg cccctgtggt tccccctaac gacatggaac agtgtttgag
     4441 ggtccgatcc gccagggaag gtgatggagt tgccggagaa acccgccgtc attggcctgg
     4501 gctatgtggg cctccctctg gcagtcgctc tgggccgcgc gcaggccgga accattgggt
     4561 tcgatgtgag ccccgggcgg gttgaggagc tccggcgagg tcatgacagc accggagaga
     4621 ccctgcccga tgcgctcgcg gccagcgata tccacttcac ctgcgacccc gccgacctcg
     4681 cggccgcgac cttctacatc gtcgccgtgc ccactcctgt caccccgcag cgccagccgg
     4741 acttgagcat ggtcgaaggg gcctctcgta ccgtcggccg cgtgctcaaa ccgggtgacg
     4801 tggtcgtcta tgaatccacc gtatacccgg gggtcaccga ggactgctgc gcccctgtgc
     4861 tcgagcgcga atcgggcctg gccgccggcc gggatttcgg attgggatac tcccccgagc
     4921 gcatcaatcc gggcgataag cagcacacgc tcgagagcat cgtcaaagtc gtcgccgggg
     4981 acactcccga gacactggag cgtgtggcgg ctctctatga gagcatcgtc cctgccggcg
     5041 tctttcgtgc cagctcgatc cgtgtcgccg aggcggccaa ggtgatcgag aacacgcagc
     5101 gcgatgtgaa catcgccctg atgaacgagc tggcgatgat cttcgaccgc atgggcatcc
     5161 ggaccaccga ggtgctcgaa gcggctcgaa ccaagtggaa cttcctgccc ttccggccgg
     5221 gcttggtcgg cggccattgt attggcgtag acccctacta cctggcttcg gcggcggaga
     5281 ccgtcgggat tcatcccgac atgatcctct ccggccggcg cacgaactcg ggcatgggtg
     5341 agttcgtggc ccgcaagacc gtgaagctcc tgcaccaagc cggacacccc gtggcgggct
     5401 ccagggtgct tgtcatgggc atgacattca aagagaacat cccggattac cggaacagtc
     5461 aggcgcccca gatcatcggc gagctacggg agtacggtgt ggagtgcttc gcccacgacc
     5521 ccatggtgga cgcggcatcc ttccagcgtt ccacggggat cgagctggtg ggcgatggcg
     5581 atctggccga gttcgatggg atcgttctgg cggtgccgca ccgggcctat atcgagctcg
     5641 gagctgtcgg cctcggccgc ctgttgcgcc ggccgggtgt gttggtcgat gtgacttcag
     5701 ctttgaagcc tgcggagatc cctgcgggta tccgctattg gagcctctag ggacgcgaag
     5761 gttttgacga gggtactgat cacgggcgga gcgggcttca tcggaagcca tctggtcgat
     5821 gcgtgtctcg ccaagggttt cgaggtccgc gtgttcgaca acttcgccac gggcaagcgt
     5881 gagaacctgg cgcatgtggc gggggatgtg gagctcgtcg aggccgatct gcgtgatctc
     5941 gatgcggttc gaaaggccat gcggggtgtc gaactggtga gccaccaggg ggcgctgggg
     6001 agtgttccgc ggagcgtggc tgatcccctg accaccaatg cggtcaatgt cgacgggact
     6061 ctccacgtgt tggtggcggc tcgggatgcc ggtgtccgac ggctcgtgtt cgccagcagc
     6121 tccagcgtct acggcgacac ggtggagctc cccaagcgcg aggagatgcc ccctcgcccc
     6181 aagagcccct atgcctgctc caagttggcg gccgaggagt actgcaagac cttccacaag
     6241 gtctatggcc tggggaccat agcgctgcgg tacttcaatg tgttcggtcc gcgtcaggac
     6301 cccacgagcc agtatgcggc ggtgatccct aagttcgtca cggccctctg tacgggcgcg
     6361 cgtcccgtgg tctatggcga tggcacccag agccgggatt tcaccttcat ccggaacgtc
     6421 gccgaggcca atctcttggc cctcaacgcc cccgaagaag ctctcgggca ggcctataac
     6481 atcgccctca atgaccaggt gaccctcctg cagatcctcg acaacctcgg gcggatcatc
     6541 ggcgtcacgc ccaacccgga gttcgcggaa gcacgggccg gcgatgttca cgactcccgc
     6601 gccgccatcg atctggcccg agagcggttg ggctatcagc ctcgtgtcgg ctgccaagag
     6661 ggcctcgagc agaccgtggc atggtaccgc gaaggcggca caggccggct ggactggggc
     6721 acgtaagggt gggatgtgct ccgccgctcc agggtttctt cgttcttcca ccaggccgtg
     6781 agcctcctcg ggcacaggtg gctcgcaccc aaacccggcg ggcccaagtg catggacccc
     6841 agttctgtag ccgaggcttt agcctcggat cacaatgcct ggagcgggtt cgagcgaggc
     6901 tgaagcctcg cctacggttg gtcgtgcgtg gcggttgggc cgcgtccgac tctaattgcg
     6961 ggccattcga cccttccccc taccctcgca accgctgggc cagacggagg agtgcggcgg
     7021 gaaccagtgc cttctggtgc tcgccgaaga tccgcacatc ggtcccgccc aggcggcgct
     7081 tgaacacatc cacgctggcc agggaacctc gagacggatc cgcgggatcg ccgatgcccc
     7141 ccaggtcata gatccggaag cctcgttccc gagcccactg catggccttc cagtggacca
     7201 ggaaaccggc cggaatgccc gtaatctccc gattcgtgcc cgcgcataga tagtaggtgg
     7261 tgggcagcgt gggatggtgg acgagcagga ttccctgggt cggctgcccc tcccagaggg
     7321 ccacatgcac actgcccagg tcatgggcca ttatggcacg cagggtcggg agaggtcgcg
     7381 agcgtaggcc tttgagctca ttgctctggg tgtagagctg ccagaagaga ccgagaagct
     7441 cctccgaagt accgtgctcg atccgcacgc ccttccgctc gcaggcacgc gcatcccgac
     7501 ggccggagga gtgcatctgg gccaggagct cttcggggga gagcgagaga tcgacgtatc
     7561 gagtgtatcc gtaggcgatg acgcgctcaa agggacgcat gtcacattga gccagcccgg
     7621 gcatggcgga ctgggcgaag ggcacccagg gccagatgcg gaggaacacc cctcgctggc
     7681 gccgcccata gcgagcgaga gcggtcaaaa cgcctcgcca cagttcgggg gagttggggt
     7741 ccctcaggat tggaccgcca tagcagatga ggccccgaaa cccggggccg tagggccgga
     7801 ggctgagcac ggccgaggcc acaggtccgg cgtcgtcgga gagaagcacg catcgcgccc
     7861 gaacgccggg ctcggcttcg atgacctgtg cccaagccgc catctgcatg gcgatggtgc
     7921 ccggctggtc ggccgcgaat gcatcgagga taccggcctc gctcggatcg atctcgcgca
     7981 gcgtcagatc catggtgtct cagtctagcc gcgcgagtga aagctggggg aggttttcgg
     8041 agggccgaat aggggtggac cagtcgaagg caggtgggac cctattcggc ccggatcgta
     8101 cccttaccgc accaccgcgg gcgccggctg cctcgcctgc cggagtagcc gctcgacccg
     8161 ctgctggatc ggcgggtgtg tctggaacag gctgagcatc atgcctcccg ccccactcag
     8221 cgggttcacg ataaacaggt gtgcggtggc cggtgtggca tggctgggga tctgctccgc
     8281 cgactgctcg agcttgagta gcgcccgcgc caggccctcg ggattccccg tcagccgtgc
     8341 cgcactggca tcggcctcat actcgcgggt ccgcgagatc cccagttgga tcagcgtggc
     8401 cgccaagggc gcgaacagga gcatcaagag ggcaaccacg ggccctcccc caccctcttc
     8461 gtcgtcccgc gccatgccgc cgaacatgct agcgaaatag ccgagctgaa ccagggtcat
     8521 gaccgcgcca gctagtgtgg ccactaacgt catcgtgagg gtgtcccggt gcttgatgtg
     8581 ggcgatctcg tgggccagaa ccccttcgat ctcgcggggc ccgaggcgat cgagtagccc
     8641 ctggttgagc gccacggcgg cgtgagcggg gctcctcccg gtggcgaagg cgttgggctg
     8701 gggatctggc agaatgtaga gccga
//
LOCUS       DAOSPL010000013         8206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_8698_length_8206_cov_2.765918, whole genome shotgun sequence.
ACCESSION   DAOSPL010000013 DAOSPL010000000
VERSION     DAOSPL010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8206
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8698_length_8206_cov_2.765918"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="PLQ54_00515"
     CDS             <1..944
                     /locus_tag="PLQ54_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91770.1"
                     /translation="RPTSSTSTPGPGRASIASESSAAASEAAVRDDGLAVVVPRGWQP
                     RDPEGNPVFPAGHTTPAHGYAEYSVPELPDGPSESSAWREGVFHGPEPDSVWGTGGSV
                     CWTGGDVTLHVPVPATECRITLPYWVGQVPGVVRVFVDGELHSEVPPNRRGTLEIHLP
                     EATAERWVAVRLRSGTWVPQEAGGGPDTRHLAVMLRDVAVEPVGERPEDEEVATVHRV
                     GRGTVLEAVRAASEGIALVVEALLRDPEAFGVAPLTEEARADGKRDGLYLAVTTTDVL
                     LYNHTSEARAIELGGRAVEVPGHAIVSVPRSSMADGR"
     gene            complement(999..1784)
                     /locus_tag="PLQ54_00520"
     CDS             complement(999..1784)
                     /locus_tag="PLQ54_00520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOS91771.1"
                     /translation="MGAVLLPAVVTLTKHQRGGCVEESAVIRLGDRDVEYRIVRSHRS
                     SRVRLKVGPATGLVVVVPVAFNAQRVPEILAGEKDWILKHVRRVEAVEAARPRIALCD
                     GGQVMFGGRPLTIQVHPRPGGLGPTVITRSDDAIHVALARTDTARLEELLLEWLQGHA
                     RSAIHAAILDLDPDRQFRFDRLCIRDQRTRWGSCSSRGELSFSWRLIMAPPEVLRYVV
                     AHELAHIRERSHSRRFWSLVGQLVPDYDAAKRWLRTHGAHLEF"
     gene            complement(1831..4512)
                     /locus_tag="PLQ54_00525"
     CDS             complement(1831..4512)
                     /locus_tag="PLQ54_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOS91772.1"
                     /translation="MAQTPGDPPTLYVSPDGQDAWSGALPQPDGAGTDGPLATVHAAR
                     DTIRAMREDGRLAAGARARVVLRGGTYWLTEPLVLEPRDSHVDYVAYPGEHPVLSGGT
                     LVGGLTREADGTWATTLPGVADGSWYPRQLFVDGRRATRAKSPNEGFYFVRDTFPPPE
                     PPGQEPDESRTAFRFEPGDIRPWVDLEDAEVVVFHSWETSRHHIASLDEDAQVVRFTG
                     PAHWHFGYFGPEQRFYVENIAEALDTPGEWHLDRRTGRLRILPEHDQDLSAAEVIAAR
                     LPLLVALTGDAELGLVVHGVRFDGLCFAHQDWTLPPGGHSDAQAAFSVPAAVMLDGAA
                     NCRFDGCEVAHVGGYGLWLRRGCHGNTITRGSFHDLGAGGIRIGEALPAAADAAESRA
                     NTVENNHIHDGGHVYAAGVGIWVAQSSHNLISHNDVHDLNYSGMSIGWNWGDEPNRTR
                     ANTIEFNHVHHVMGSMLNDGGAIYTLGVSPGSVIRGNLLHDVWPYSNLGWGIYLDATT
                     AGYTVSHNVVYNTYSGGVMYNNGGHDNVIDNNVFAFSAAEALWPCFEPRPCDFRRNIV
                     YLSQGELLVPFAEHWLRDRIAAGESLGVWDENVYFRTGARPLKFLGRDFADWQALGLD
                     ARSQIADPEFLDVAAYDFRLAPTSPAIALGIESIDVSSVGLYGDPEWVRQPSLTPHEP
                     VELSRIGGRARPQSIGDGFETRPPGSRPSRATVSGERHGASIRIAAEVAASGDQSLAI
                     RDAAGLELRWEPHFYYQLDLTEGMARQSFDLWLGAGGELFTEWRDDTAYPGCVGPSVA
                     VNSAGQVSAGGTVLTAIPLGQWVHFDIECALGNDPPSPFVLTVTVPGDDGPRRFTDLP
                     VPGADFHALRWVGFVSTADADTTSYIDNVEVDAVPAP"
     gene            4741..5874
                     /locus_tag="PLQ54_00530"
     CDS             4741..5874
                     /locus_tag="PLQ54_00530"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS91773.1"
                     /translation="MLVVVKPTNACNGTCRYCSADSGKTSSPMRVDQLPALFGAFAEW
                     LCAARGDGLNFLWHGGEPMLMGPAFYWHVLEAQREAFDANANRVTNSIQTNLSLLTPE
                     WIPLLRELLGRSRVGTSFDPVPGWRGLRDGVDYDERWLEATELLRRSGIRYGVVYVVH
                     RESLGHAARIYRYFRNWDPGASTRFNPLYAQGRAAAPADGALGLTADEYGEFLVELLD
                     AWREDGCRARVAPLTEWWEAWRGQRSRLCCDCSGRCSDSHLGVTPDGSVYGCGRCIDS
                     GVGRFGNLFEEPLGSILASRARSMMVGRAEGLLHGPCQDCEWWALCGGGCPNDGLIAY
                     GDLTRETGFCAARKRLFGRLRELLGPPALVAPSGESSCEEAAE"
     gene            5871..6749
                     /locus_tag="PLQ54_00535"
     CDS             5871..6749
                     /locus_tag="PLQ54_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91774.1"
                     /translation="MSEGRATALVDLPGEWFGLPEGSRAVLEMAETELAAADWLSSLE
                     VGRAVRVSLPEDAPWGPALREYPGDLSIRALTGPPAALPTAVLARGECASAEIPPGQS
                     ALERLRACGEAGVPARLADLDLWPGPDLAAALDFYITCPTLESPVEPWHSLAQAVSGG
                     WPCDLWHAEEAVVGRNIYISVQGLATLSARFDRRERWFGAGGEGLEGWRGSALWRELE
                     GYRRALFRTRAECAFCAAFPWCGGFWVAPGLEDHERCEVWRGLMAHLAKMAREAGASA
                     TSSTNPMGTPCGRGTS"
     gene            6827..7132
                     /locus_tag="PLQ54_00540"
     CDS             6827..7132
                     /locus_tag="PLQ54_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91775.1"
                     /translation="MADEREPARSVEGARDPEMAGLSPICRRAFLGRIAKAAIGTGVV
                     AGAATLSGCLGLYGYVDFGYGDYGDHGGYGDYYNYGDYGNYANYGDYANYADYANYY"
     gene            7320..7559
                     /locus_tag="PLQ54_00545"
     CDS             7320..7559
                     /locus_tag="PLQ54_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91776.1"
                     /translation="MAEARPGVAAPQEGLPQPAEALSPICRRAFLSHAARAALGAGLV
                     AGAGVLSGCLGGYYADYPNYANYPDYANYADYANY"
     gene            complement(7570..>8206)
                     /locus_tag="PLQ54_00550"
     CDS             complement(7570..>8206)
                     /locus_tag="PLQ54_00550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS91777.1"
                     /translation="ALEALRAAGAIPHTEYDTARFLAHREAVAREFEIPWTAISPRMQ
                     RLLYALNAISQPRVMVAAGVFCGNTFISNAGAGVGPGACYQAHALIGVEIKPEEAARA
                     ERNVRRIDPTGIARVLAADALDVVAKLEEPIDLLYLDADGHRGQGKGIYLEILLAALS
                     KMPAGSLVLAHNSVNGGDPVQPYLDYVRNPANCRASLNVILDPEGLEVTVL"
BASE COUNT         1347 a         2560 c         3017 g         1282 t
ORIGIN      
        1 ccagacccac cagcagcacc agcacaccgg ggccgggcag ggcgagcatc gcgagcgaga
       61 gcagcgcggc ggccagcgag gcggcggttc gcgacgacgg cctggcggtg gtggtcccgc
      121 ggggctggca gcctcgcgac cctgagggca acccggtgtt cccggccggg cacacgaccc
      181 cggcccatgg gtatgcggag tactcggtgc cggagctacc cgatgggccc agcgagtcct
      241 ctgcatggcg tgagggcgta ttccacggcc ctgagcccga ctcggtgtgg ggtaccggcg
      301 ggtcggtctg ctggaccggc ggagacgtaa ctctccatgt gcctgtgccc gcgaccgagt
      361 gccgcatcac gctcccatac tgggtggggc aggtgcctgg agtcgtgcgt gtcttcgtgg
      421 atggtgagct ccacagtgag gtgccgccga accggcgggg aaccctggag atccacctac
      481 ccgaggcgac ggccgagcgt tgggtggccg tccgtctgcg gagtggaacg tgggtgccgc
      541 aggaagcggg gggaggcccg gacacgcgcc acttggcagt gatgctgcgc gatgtggccg
      601 tcgaaccggt gggtgagcga cccgaagatg aggaagtcgc cacggttcac cgcgtgggca
      661 gagggacagt gctcgaggca gtccgtgcgg cgagtgaggg cattgcgctg gtagtggagg
      721 ccctccttcg agaccccgag gcattcggcg tggctcctct caccgaagag gcccgggcag
      781 acgggaagcg cgacgggctc tacctggccg tcaccacgac cgatgtgctc ctgtacaacc
      841 acacgagcga ggcgcgggcg atcgagctcg gcggtcgagc cgtcgaggtg ccggggcacg
      901 ccatcgtgag tgtgccgaga agctcgatgg cagatggcag atgagagata acagatggtc
      961 ggtgccggac gaggccgccg gccacgcagt gggctcagtc agaactcgag gtgggcgccg
     1021 tgggtgcgga gccaccgctt ggcggcgtcg tagtcgggca ccagctggcc caccagactc
     1081 cagaaccgtc gggagtggct acgctcacga atgtgggcaa gctcgtgagc aaccacgtat
     1141 cggagtacct ccggcggggc catgatcagg cgccagctga aggagagctc cccacggctg
     1201 gagcagctgc cccagcgggt tcgctgatcg cggatgcaca gacggtcgaa gcggaactga
     1261 cggtcggggt cgagatcgag gatcgccgcg tggattgcgg atcgtgcatg gccttggagc
     1321 cattcgagga gcagttcctc gagccgcgcc gtgtccgtgc gggcaagagc gacgtgaatg
     1381 gcgtcatcgg accgagtgat gacggtgggg cccagaccgc ccggccgggg gtgcacctgg
     1441 atggtgaggg gccggcctcc gaacatgacc tgcccgccat cacacagcgc aatccgcgga
     1501 cgtgcggcct ccacggcctc gacgcgccgg acgtgcttga gaatccagtc cttctcgccg
     1561 gcgagaatct cgggcactcg ctgggcgttg aacgccacgg gcaccacgac caccagaccg
     1621 gtggcgggcc ccaccttgag gcggacgcgc gacgatcggt ggctccgcac gatgcggtac
     1681 tcgacgtcgc gatcacccag ccggataacg gcggactcct ccacacaacc acctcgttgg
     1741 tgcttcgtca gcgtgacaac agcgggaagc aggacggcac ccatggcggg cgactcgacg
     1801 cgacgggccg cacagcccct tgccgcgcca tcacggggcc ggcacggcat cgacctccac
     1861 gttgtcgata taggacgtgg tgtcggcatc tgcggtgctg acgaacccga cccaccgcag
     1921 cgcatggaag tcggcgcctg ggaccggcag atcggtgaac cggcgcggcc catcatcgcc
     1981 gggcacggtt acggtgagca caaagggaga cggcggatcg ttgcccaggg cgcactcgat
     2041 gtcgaagtgg acccactggc cgagagggat tgcggtgagc accgtcccgc cagcggagac
     2101 ctggcctgcc gagttgacgg ccacgctcgg tccgacgcac ccgggatagg ccgtgtcgtc
     2161 acgccactcg gtgaacagct caccgccggc ccccagccac aggtcgaagc tctgacgggc
     2221 cataccctcc gtgaggtcca gctggtagta gaagtggggc tcccaacgga gctcgagacc
     2281 agcggcgtcg cggatggcca ggctctggtc gccgctggcg gccacctcgg cggcgatacg
     2341 gatcgaggca ccgtggcgct cccctgagac ggtggctctc gaggggcgcg agccaggcgg
     2401 gcgggtttcg aagccgtcgc caatggactg cggccgcgcc cgtccgccga tgcgagagag
     2461 ctcgacaggc tcgtggggtg tcaacgaggg ctgacggacc cactcgggat cgccatagag
     2521 gccgacgctc gagacgtcaa tcgactcgat gcccaacgcg atagcgggcg aggtcggggc
     2581 gagccggaag tcgtacgcgg ccacatcgag gaactccggg tcggcgatct gtgacctcgc
     2641 atccagcccc agcgcttgcc agtcagcgaa atcgcggccg aggaacttca gcggccgggc
     2701 gccggtccgg aagtagacat tctcatccca cacacccagt gactcgcctg ccgcgatccg
     2761 atcccgaagc cagtgctcgg cgaacggcac cagcagctcg ccctggctca ggtacacgat
     2821 gttccggcgg aagtcgcacg gccgtggctc gaagcagggc catagggcct cggcggccga
     2881 gaacgcgaac acgttgttgt cgatgacgtt gtcgtggccg ccgttgttgt acatgactcc
     2941 gcccgagtat gtgttgtaga caacattgtg cgacaccgtg tagccggcag tggtggcgtc
     3001 caggtagatg ccccatccta gattgctgta gggccacaca tcgtggagga gattgccgcg
     3061 tatcacactg ccgggcgaca cgccgagcgt gtagattgct cccccatcgt tgagcatact
     3121 ccccatcaca tggtgcacgt ggttgaactc gatggtgttg gcgcgcgtgc ggttgggctc
     3181 atcaccccag ttccagccga tggacatgcc ggagtagttg aggtcgtgga catcattgtg
     3241 cgagatgagg ttatggctgc tctgtgcgac ccagatgccc acgcccgcgg cgtagacatg
     3301 gccgccgtcg tggatgtgat tgttctcgac agtgttggcg cgcgattcgg cggcatcggc
     3361 ggccgccgga agggcctcgc cgatccggat cccgccggcc ccgaggtcgt ggaagctgcc
     3421 gcgtgtgatc gtgttgccgt ggcaccctcg gcggagccag aggccgtatc cgcccacgtg
     3481 ggcgacctcg cagccatcga agcggcagtt ggcggcgccg tcgagcatca ccgccgcagg
     3541 cacggagaac gctgcctggg cgtcgctgtg gcccccgggc gggagggtcc agtcctggtg
     3601 cgcgaagcaa aggccatcga accggactcc gtggaccacg agcccgagct ccgcgtcgcc
     3661 ggtcagggcc acgagcagcg gcaagcgcgc tgcgatcacc tcggcagccg aaaggtcctg
     3721 atcatgttcc ggcagtatgc gcagtcggcc ggtgcggcgg tcgaggtgcc actcgccggg
     3781 cgtgtcgagc gcctcggcga tgttctccac gtagaagcgc tgctcggggc cgaagtagcc
     3841 gaagtgccaa tgggccggtc cggtgaaccg aaccacctgg gcgtcctcat cgagggaggc
     3901 aatatggtgg cgcgaggtct cccaggagtg gaagacaacg acttcggcat cctcgaggtc
     3961 aacccagggc cggatgtcgc cgggctcgaa gcggaaggcc gtgcggctct catcgggctc
     4021 ctgaccgggc ggctcagggg gtggaaacgt gtcacgcacg aagtagaacc cctcgttggg
     4081 tgacttggcc cgtgtggcac gacggccgtc cacgaagagc tgccgggggt accagctgcc
     4141 gtcggcgacg ccgggtagcg tggttgccca ggtgccatca gcttcgcggg tgagaccgcc
     4201 gaccaatgtt ccaccactga gaactgggtg ctccccgggg taggcgacat agtcgacatg
     4261 agagtcgcgc ggttcgagaa caaggggctc ggtcagccag tatgtgccgc cgcgcagcac
     4321 aacgcgagcg cgggcaccgg ctgcgagccg gccatcttcg cgcatggcgc ggatggtatc
     4381 acgcgcagca tgcacggtgg ccaaaggccc gtcagtgccc gcgccatcgg gctggggcaa
     4441 cgcacccgac caggcatctt gcccatcggg cgagacgtag agggtcggcg ggtcgccagg
     4501 tgtctgagcc atcgacggca gacacggggc cagtacacac tgcgcgcacg cgagcagaag
     4561 ccgccgggca tgggcagagc gggcacgcat ttcgttcccc ccagcgaaac gcagagtgcg
     4621 gggtagttcc aagcggggac accgagtgcc tgccccgggc attcgctcgg gtggtcacgg
     4681 cactgatggc ggtgtcgagc gtcctagaga tggatgcgcg aacagggagg tgtcggccca
     4741 gtgctggtgg tggtgaagcc gacgaatgcc tgcaacggta cgtgccggta ctgctcggcg
     4801 gattcgggca agacctcatc gcccatgcgt gttgaccaac tccctgcgct ctttggcgcc
     4861 ttcgccgagt ggctgtgtgc cgcgcggggc gatgggctga acttcctctg gcacggcggc
     4921 gagcccatgc tcatgggccc agcgttctac tggcatgtgc tcgaggcgca gcgcgaggcg
     4981 ttcgacgcca acgcgaaccg ggtcacgaac tcgatccaga cgaacctgtc gctgctgacg
     5041 ccggagtgga tccctctgct ccgcgagctg ctcgggcggt cgcgcgtggg cacgtcgttc
     5101 gacccggttc caggctggcg cgggctgagg gacggcgtgg attacgacga gcgctggctc
     5161 gaggcgaccg agctcctgcg gcggtcgggc atccggtacg gcgtggtcta cgtggtccac
     5221 cgggagagcc tgggccacgc cgcgaggatc tatcgatact tccgcaactg ggatcccggg
     5281 gcatcgacgc gcttcaaccc actctacgct caggggaggg cggcggcccc ggctgacggc
     5341 gcgttggggc tcacggcgga cgagtacggc gagtttctgg tggaactcct ggatgcatgg
     5401 cgggaagatg gatgccgcgc gcgggtggcg ccgctcaccg agtggtggga agcctggcgc
     5461 gggcagcggt cgcggctgtg ttgcgactgc agcgggcgat gctcggacag ccatctgggc
     5521 gtcactccgg atgggtcggt ctacggctgc ggtcggtgca tcgacagcgg ggtagggcgg
     5581 tttggcaacc tgtttgaaga gccgctgggt agcatcctgg cgagccgtgc gcgaagcatg
     5641 atggtcggac gggctgaggg acttctgcac ggcccgtgtc aggactgcga gtggtgggcg
     5701 ctctgcgggg ggggctgccc caacgacgga ctgatcgcct atggagacct aacacgagag
     5761 accggtttct gcgcggcgag gaagcgcctg ttcgggcggc tgcgcgagct gctcgggccg
     5821 ccggcactgg tcgcaccgag cggcgagtcg tcgtgcgagg aggcggccga gtgagcgagg
     5881 ggcgcgccac agcgttagtg gacttgccgg gcgagtggtt cggactgccg gaaggtagcc
     5941 gcgctgtgct ggagatggcc gagacggagc ttgccgcggc ggactggctg tcatcgctgg
     6001 aggtcggtcg cgcggtgcgg gtcagccttc ccgaggacgc accatgggga cccgcgttgc
     6061 gggaataccc gggcgacctg tcgatccggg cgctcaccgg tccaccggcc gcgctcccca
     6121 ctgccgtgct ggcgcggggg gagtgcgcat cggccgagat cccgccgggc caaagtgcgc
     6181 tcgagcgtct ccgggcttgc ggtgaggcgg gtgttccggc ccggctcgcg gacctagacc
     6241 tgtggccggg acccgacctg gcggcggcac tggacttcta catcacctgc ccgactctcg
     6301 agtcgcccgt ggaaccgtgg cactcgctgg cgcaggcggt gtccggcggc tggccatgtg
     6361 acctatggca cgccgaggag gccgtggtgg gaaggaatat ctatatctcc gttcagggtt
     6421 tggcgacact gtcggcccgt ttcgaccgcc gcgagcggtg gttcggcgct ggtggtgagg
     6481 gcttggaagg ctggcgcggc tcggcactgt ggcgcgagct cgaaggctac cggcgtgcgc
     6541 tgttccgaac gcgcgcagag tgcgccttct gtgcggcgtt cccttggtgt ggcggcttct
     6601 gggttgcacc cggccttgag gaccacgagc gctgcgaggt gtggcggggc ctgatggccc
     6661 atctggcgaa gatggcgcgc gaggccggag cgtcggccac cagcagtaca aaccccatgg
     6721 gaacgccatg cggccgcggc acgtcgtagg cgtggagggg aatccgctcc gagggcatat
     6781 cgactgcgag ttgggagagc gcacgcggtg gagggagtgg gcagcgatgg cagacgagcg
     6841 cgaaccagcc cgatcggttg agggggcacg agatccggag atggctgggc tgagccccat
     6901 ttgccgacgg gcgttccttg gccggatcgc caaagcagct atcggcaccg gcgtggtcgc
     6961 tggggcggca accctgtcgg gttgtctggg gctgtatggc tatgtcgact ttggttacgg
     7021 cgactatggt gaccatggcg gctatgggga ctactacaac tacggtgact acggcaacta
     7081 cgcgaactat ggcgactacg ccaactacgc ggactacgcg aactactact gaggccggag
     7141 tgcccggggg caggacatgt cctgaggtcc tcgacgactt ccgcggatgc actctgcata
     7201 gcggaatcgg gaaccatcgg cgacattcct gtgtaggatt agtggagtta cggaggggtt
     7261 cgtcggcaga tcccgacgcc gcggactgtg ctggctgccc cgggagagga tgaacggcga
     7321 tggccgaggc gaggcctgga gtcgcggcgc cacaagaggg gctcccccaa ccagcggagg
     7381 cgctcagccc gatctgtaga cgggcattcc tgagccacgc ggcaagggcg gcgctggggg
     7441 ccggactggt cgccggtgcc ggcgtgctgt ccggatgcct ggggggctac tacgcggact
     7501 acccgaacta tgccaactac cccgactacg ccaactacgc ggattacgcc aactactagc
     7561 tcacccgcat cacagcaccg ttacctccag gccctccggg tccaagatga cgttgaggct
     7621 ggcgcggcag ttggccgggt tgcggacata gtcgaggtat ggctgcacag gatcgccgcc
     7681 gttgacgctg ttgtgcgcga gcaccagtga accggccggc atcttgctga gcgcagccag
     7741 caggatctcg agatagatgc ccttcccttg acccctgtgc ccatcggcgt cgaggtagag
     7801 caggtcgatg ggctcctcga gcttcgcgac cacgtccagc gcgtcggcgg cgagcactct
     7861 ggcgatgccg gtggggtcga tccggcggac gttgcgctcg gcgcgcgcgg cctcctccgg
     7921 cttgatctcg acaccgatga gcgcatgggc ctggtagcac gctcccggcc caacgccggc
     7981 cccggcattg gagatgaacg tgttcccgca gaacaccccg gcagcgacca tcacgcgggg
     8041 ttggctgatg gcgttgaggg catagagcag gcgctgcatc cggggcgaga tggccgtcca
     8101 aggtatttcg aactcacgcg ccacggcttc gcgatgagcc aggaaccggg cagtgtcata
     8161 ctccgtgtgt gggatggcac cggcggcccg cagcgcctcg agcgcg
//
LOCUS       DAOSPL010000014         8040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_8946_length_8040_cov_2.943519, whole genome shotgun sequence.
ACCESSION   DAOSPL010000014 DAOSPL010000000
VERSION     DAOSPL010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8040
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8946_length_8040_cov_2.943519"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>558
                     /locus_tag="PLQ54_00555"
     CDS             <1..>558
                     /locus_tag="PLQ54_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOS91778.1"
                     /translation="LFEREYLPCAVAPEVLAANQRSPEQQLASLRFVLSTVDPRPTVL
                     GVLTLGKDPPRHIPGAYVQFVRFDGPTLSDPIRAQAEVSGPMSQMLRVLDELLEAHVS
                     VAVDLASGRTEARAPDYPISALRQIARNAVLHRTYDRSNAPVRISWFADHVGIISPGG
                     PYGVGSRMDITATRTSFPSQTKRFS"
     assembly_gap    559..568
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <569..762
                     /locus_tag="PLQ54_00560"
     CDS             <569..762
                     /locus_tag="PLQ54_00560"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS91779.1"
                     /translation="FGTPGVTDYRNPNLARVMRDLGYVQRFGVGIEEARRALRGNGNP
                     ELEFQVEDTFVAAVLRCRE"
     gene            759..1750
                     /locus_tag="PLQ54_00565"
                     /pseudo
     CDS             759..1750
                     /locus_tag="PLQ54_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015171502.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(1777..1956)
                     /locus_tag="PLQ54_00570"
     CDS             complement(1777..1956)
                     /locus_tag="PLQ54_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91780.1"
                     /translation="MWSFSDRWAQHVRLGCFSPNYRSYFTGLISEVLVYTRVLTQDEQ
                     DLVRAHLMAKWGWRG"
     gene            complement(1940..4828)
                     /locus_tag="PLQ54_00575"
     CDS             complement(1940..4828)
                     /locus_tag="PLQ54_00575"
                     /inference="COORDINATES: protein motif:HMM:NF015387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOS91781.1"
                     /translation="MRAAWLAATVALSAVAAQSATIHVSPDGDDSRDGLAPGRAMRTI
                     QAAVNRLGPGDTCLVHEGVYREAVAFPRSGEAGKPITLRATSKGVVVTGCEPISGWER
                     HRGDIWKAPMPWNMGLGRNQVFSGDQVLIEARYPNEPAPGLGMYVSGLSPLWPTFGEF
                     SIPRETVAEQPGRIVSRLLDGQPDDYWKGALYYGIHYEGWAAQTGVVESSKSGEILVG
                     DRTRTWWFASAGYSPEEGRGMLVGHMNALDRAGEWHWQDGTLYLIPPEGTEPSAIEAK
                     RRALAFDLSGREHIRIEGFAVRAASMRLEDSAYCVVDGCSLSYISHYTHHYDIGQIEP
                     GRDTIKSGETGIFVGGHDNAFLNCSVRFSAGAGFHLRGYHHTIHNCLIDEVSYTAHYL
                     NAITDAVSDFGDYEGLLVGGHVITYNTMRNAGRHFFNIHGNGTSLASRTRGPMDYMAT
                     LFVGNHLYNGMLQTRDAGMLTGYYCSGGTLNGLRTQLAYNVMHDCYDIFAMRIGVLGI
                     VYLDAGTCNVDVHHNLLWAVPGSLQRGFWFNTMCVDIRERDNVFHPNFERTCAELKAE
                     DFPEEEPFRFGHDFFRPPPLPVWPQLERKWLKAEARGLADGDVVPLGDVDFGQGWQSA
                     VVPFASDVKDMNTDKSARQAPRHQKATDPLVLEATTNDGSEARIGRQWTFVHTVHDGA
                     WLRFAGVPLGEGYRRLRVVYGTDRTEPRWLEVHLDSADGPLAGRVDLPQTDVPRGWPV
                     QIYREAIGGISTEATGTRDVFIVFHTGDGGPVGEFEYFRFEQYRGQIALQKDEVRYEL
                     RVGTPQGDLVGVLYPRATGSAQGYRPLVTSLEPASGRQPLFLVVRSNFPGGTGAIAGV
                     SLEKARRPIDWTGVGIQPRGYGAGTMVLPGPTNRPQARPGDAYASNASPPRKRPLCLA
                     TALDGPLSVDGRLDDWLAAGPREALELGPSRPTRYGDGRAAPGRVELQ"
     gene            complement(4900..5475)
                     /locus_tag="PLQ54_00580"
     CDS             complement(4900..5475)
                     /locus_tag="PLQ54_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOS91782.1"
                     /translation="MRVVAFNGSPRKDGNTARLVRRVFAELEAEGIETELVQLGGEHI
                     HGCVACYNCFTNKDRRCAIADDKANTFIERMLEADGIILGSPTYFADITPETKALIDR
                     AGLVARANGDLFQRKVGAAVVAVRRGGAVHAFDSINHFFLITQMIIPGSIYWNMAIGR
                     EKGEVESDEEGQRTMETLGRNMAWLLKKLNT"
     gene            complement(5492..7789)
                     /locus_tag="PLQ54_00585"
     CDS             complement(5492..7789)
                     /locus_tag="PLQ54_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOS91783.1"
                     /translation="MTERHSLIIARELGVAPHQVSSTAALLSEGATVPFIARYRKEVT
                     GALDEVAIAAIRDRLGQLEELDKRRAAIIKSLDERGLLTSELRAQVDAAPSMAVLEDI
                     YLPYRPKRRTRATVARERGLEPLALALLEQTEAMDPLREAATFIDEDKGVASAEEALA
                     GARDIIAERVSEDQQARSNMRRLYATQSNISSRVARDRADEGAKYRDYFEWTEPIANA
                     PSHRILAMMRGENEGFLRLTVAPPEDAAIRQLRAQFVTGQNPASEQVALAVEDGYKRL
                     LAPSMETEIRGELKRRADEQAIRVFASNVRELLLTAPLGQKRVVAIDPGLRTGCKLVC
                     LDEQGKLVHHDVFYIHDSGPQAAKAGPKLRDIVERFRPAAIAIGNGTASRETEAFVRG
                     LGLPDSVAVVVVNESGASVYSASEVAREEFPDQDVTVRGAVSIGRRLMDPLAELVKID
                     PKSIGVGQYQHDVDQAALRRALDDVVVSCVNAVGVDVNTASKQLLTYVSGLGPQLAQN
                     IVNHRNENGPFRSREQLMAVPRLGPKAFEQAAGFLRVRGGDNPLDASAVHPESYHIVN
                     AMARDAGCTVADLLSQESMRARIDLSRYVSGPVGMPTLHDIIAELAKPGRDPRQVFEA
                     FAYADGVVKIEDVRPGMVLPGIVTNVTAFGAFVDVGVHQDGLVHVSQLADRFVRDPNH
                     VVRVGQRVRVRVLEVDLARRRISLSMRSGTISTAPSPALPKDRGRRRSEPRREQPQPV
                     PEPQPTNWFTEALRKAEEQKRGRGE"
BASE COUNT         1285 a         2804 c         2531 g         1410 t
ORIGIN      
        1 ggctcttcga gcgcgagtac ctcccatgcg ccgtcgcccc cgaggtgctc gccgcgaacc
       61 agcggagccc cgaacagcaa cttgccagct tgcgcttcgt gctgtcgacc gtcgatccgc
      121 gccccaccgt gttaggcgtg ctgaccctgg gcaaagaccc tccgaggcat attcccgggg
      181 cctacgtgca gttcgtacgg ttcgatgggc cgaccctcag cgatcccatc cgggcgcagg
      241 ccgaggtctc tgggccgatg tcgcagatgc tccgcgtgct cgacgagctg ctagaggccc
      301 acgtgtccgt cgccgtagac ctggcctccg ggcgaactga ggcgcgggcc ccggactacc
      361 cgatctcagc tctgcgccag atagcgcgga atgcagttct gcaccgcacg tatgataggt
      421 ccaatgcgcc cgtgcggatc tcctggttcg cggaccacgt tgggatcatc agccccggcg
      481 gcccctacgg cgtcggctcc cgcatggaca tcacggccac gcgcaccagc ttcccatcgc
      541 agaccaagcg tttcagcann nnnnnnnnat ttcgggacgc ccggcgtgac tgactaccgc
      601 aaccccaatc tggccagggt catgcgagat ctgggctacg tgcagcgctt tggggttggc
      661 atcgaagagg cccgccgcgc gctccgtggg aatgggaatc ctgagcttga gttccaggtg
      721 gaggatacgt ttgtcgctgc agtgctgagg tgccgtgagt gagcgccccg atcatcgcgt
      781 tcttcaacaa caagggcggg gtcggcaaga cgtcggctgt gtaccacctc gcatggatgt
      841 acgccgaact gggccacacg gtgctcgctg ccgatcttga ccctcaggca aaccttatgg
      901 cggccttcct ggatgaggac agcctggaat ctctctggga tgaccgcgac cgcccgaaca
      961 ccatctaccg cgcgattcgc ccgctgatcc gcggcactgg cgatgtcacg gcatccgaga
     1021 cgctggcggt cgccgatggg ctctcgctgc tggtggggga tctagacctc tcgctgatgg
     1081 aagatgacct atcggacgca tggcccgggg cgatggacgg cagggagcgc gcattccggg
     1141 ttctggcgtc gtttggaccg tgctgcagag gtctgccgcg gatgccgccg catcgcttgt
     1201 gctcgtcgat ctgggcccca acctcggcgc gctgaacaga gcagctctga tcgcggccga
     1261 ccatatcgtc gtgccgttgt gcgcggatgt gttctcgcta aggggcctct cgaacttggg
     1321 cccaaggctc cgggagtggc gggagcagtg gtcagatcgc cggtctcggt gtcccacggg
     1381 agctccggag attccggaag gcaccatgca gcccctggga tacgtcgtgc tccagcatag
     1441 catccgcctg gaccggccgg cgcgcgcgta cgagcgatgg gtcgaccgca tcccgacagt
     1501 ctatgcagag gcagtgctca accagcccgg cgcgtctgcc gacagcttcg catcggaccc
     1561 caactgcctt gggctactga agcactaccg cagccttatg ccgatggcgc atgaggcccg
     1621 gaagcccatc ttccacctca ggccggccga cggggccatc ggcgcacaca tgcagggcgt
     1681 gcttgccgcg cgtcaggact tcgagatcct tgcgcgcagt atcgccgaac gacgcggatt
     1741 gtggagctag aggcagtacc accactggca tgctctctag cctctccatc cccacttcgc
     1801 catcaggtgc gcacgcacga ggtcctgctc atcctgcgtg agtacccgag tgtagacgag
     1861 cacctccgag atgagccccg tgaagtagct ccggtagttc ggcgagaagc acccgagccg
     1921 cacgtgctgt gcccagcggt cactgaagct ccacacggcc tggcgcggcc ctcccatctc
     1981 catacctggt aggccggcta ggcccgagct ccagggcctc ccgcggcccc gcggcgagcc
     2041 agtcgtccag tcgaccgtcg acgctcagcg ggccatcgag cgcggtggcc agacacaatg
     2101 ggcgcttccg aggcgggctc gcgttgctcg catacgcatc cccggggcgc gcctgcggcc
     2161 ggttcgtggg ccccggaagc accatcgtgc cggcaccgta gccccgaggc tggatgccga
     2221 caccggtcca gtcgatgggc ctccgggcct tctcgagact cacgcccgcg atggcacccg
     2281 tcccccccgg aaagttggat cgcacgacga gaaacaacgg ctgccttccg ctggctggtt
     2341 ccagcgacgt cacgagcggc cgatagcctt gggcgctacc cgtcgcgcgc gggtagagca
     2401 cacccacgag gtcgccctgg ggcgtgccca cgcgtagctc gtagcgcacc tcgtccttct
     2461 gaagcgcaat ctggccgcga tactgctcga accggaagta ctcgaactcg cccaccgggc
     2521 cgccgtcgcc cgtgtggaag acaatgaaca catcgcgcgt cccagtcgcc tcagtgctga
     2581 tccctccaat cgcctcccgg tagatctgca ccggccagcc ccgcgggacg tcggtctgcg
     2641 gcagatcgac gcgcccggcc agggggccgt cggccgagtc caggtgtacc tcaagccacc
     2701 gcggctccgt ccggtcggtt ccatagacca cccgcaagcg ccggtacccc tcaccgagcg
     2761 gcacgccggc gaaccgcaac cacgccccat cgtggaccgt gtgaacgaat gtccactgcc
     2821 taccgatccg ggcctcactg ccgtcgttgg tggtcgcctc gagcaccaat gggtccgtcg
     2881 ccttctggtg ccgcggcgcc tggcgagcgg acttgtccgt gttcatgtcc ttcacgtcgc
     2941 tcgcgaaagg caccaccgcc gactgccagc cctggccaaa gtcgacgtcg ccgagcggca
     3001 cgacgtcgcc gtcggctaag ccgcgcgcct cggccttgag ccacttccgt tcgagctgcg
     3061 gccacaccgg cagtggcggc ggcctgaaga agtcgtggcc gaaccggaag ggctcctcct
     3121 ccggaaaatc ctcggccttc agctcggcac acgtgcgctc gaaattgggg tggaagacat
     3181 tgtcgcgctc gcggatgtcg acacacatgg tgttgaacca gaacccgcgc tgtaatgagc
     3241 ccggcaccgc ccacagcagg ttgtggtgca catccacgtt gcaggtgccc gcgtcgaggt
     3301 agacgatgcc gagcacgccg atgcgcatcg cgaagatgtc gtagcagtca tgcatcacgt
     3361 tgtaggccag ttgtgtgcgc aggccgttga gcgttccacc gctgcagtaa tagcccgtca
     3421 gcatgccggc gtcccgggtc tgcaacatgc cgttgtagag gtggttcccg acgaacaggg
     3481 tagccatgta gtccatgggc ccacgggtgc gtgaggccag gctcgtgcca ttgccgtgga
     3541 tattgaagaa gtggcgtccg gcgttccgca tcgtgttgta cgtgatcacg tggccgccga
     3601 cgaggaggcc ctcgtagtcg ccgaagtccg acaccgcgtc ggtgatggcg ttgagatagt
     3661 gtgcggtgta gctgacctcg tcgatcaggc agttgtggat ggtgtggtgg tacccccgca
     3721 gatggaaacc ggcgcccgcg ctgaagcgca cgctgcagtt gaggaacgcg ttgtcgtgcc
     3781 cgccgacgaa gatgcccgtc tcgcccgact tgatggtgtc gcgcccgggc tctatctgcc
     3841 cgatgtcgta gtggtgggtg tagtgtgaga tgtagctcag actgcatcca tcgaccacgc
     3901 agtaggccga gtcctccagc cgcatcgacg cggcccgcac ggcgaatccc tcgatgcgga
     3961 tgtgctcccg cccgctcaga tcaaaggcca gcgcacgccg cttcgcctcg atggccgacg
     4021 gctcggtccc ctcgggcgga atgaggtaca gcgtgccgtc ctgccagtgc cattcacccg
     4081 ctcggtcgag ggcgttcatg tggccgacga gcatcccccg gccctcctcc ggcgagtagc
     4141 cggccgaggc aaaccaccag gtccgggtgc ggtcgccgac aaggatctcg ccagacttgg
     4201 agctctccac cactcccgtc tgcgccgccc agccctcgta gtggatgccg taatagagcg
     4261 cgcccttcca gtagtcatcg ggctggccgt cgagcagcct gctgacgatg cgcccgggct
     4321 gctcggccac tgtctccctg gggatcgaga actcaccaaa tgtcggccaa agcggcgaca
     4381 ggcccgacac gtacatcccg agccccggag cgggctcatt cgggtagcgt gcctctatca
     4441 gcacctggtc accactgaac acctggtttc ggcccaggcc catgttccat ggcatgggcg
     4501 ccttccagat gtcgccgcgg tgccgctccc agccgctgat gggctcgcac ccggttacga
     4561 cgacgccctt gctcgtcgcc cggagcgtga tgggcttccc ggcctcccca gatcgaggaa
     4621 acgccacggc ctcgcggtac acgccctcgt gcacaaggca cgtgtctccg gggccaagcc
     4681 ggttcacggc ggcctggatg gtgcgcatcg cccgacccgg cgcgagcccg tcccttgagt
     4741 cgtccccgtc cggcgagacg tggatcgtgg cactctgagc cgccactgcc gacagcgcca
     4801 ccgtggccgc cagccaagcc gctctcatcc gtaccgacct ccaacttccg tcagcgagtg
     4861 agtggccgcg gcgccaacag gccagtgaca gactgacggc tacgtgttga gcttcttgag
     4921 cagccaggcc atattccgcc ccagggtctc catcgtccgc tgaccctctt cgtcgctctc
     4981 gacttcgccc ttctcgcggc cgatggccat gttccagtag atggagccgg ggatgatcat
     5041 ctgggtgatt aggaagaagt ggttgatcga atcgaaggca tggacggccc cgccccgccg
     5101 gaccgccaca acggccgcgc cgactttgcg ctgaaacaag tccccgttgg cccgcgccac
     5161 gagcccggcg cggtcgatta gcgccttggt ctcgggcgtg atatcggcga agtaggtcgg
     5221 cgaccccagg atgatgccgt cggcctccaa catcctctcg atgaacgtgt tcgccttgtc
     5281 gtcggcgatg gcgcaccgtc tgtccttgtt cgtgaagcag ttgtagcagg cgacgcaccc
     5341 atggatgtgc tcgccgccga gctgcaccag ctcggtctcg atgccctcgg cctcgagctc
     5401 ggcgaagacc cgccgaacca gccgggcggt gttcccatcc ttgcgcggac tcccgttgaa
     5461 ggcgacgact ctcatggtgg tgctccttgt cctactcgcc gcgcccacgc ttctgctcct
     5521 cggccttcct gagggcctcc gtgaaccagt tcgtgggctg tggttctggc acaggctgtg
     5581 gttgttcgcg ccggggttcg ctccgacggc gcccccgatc cttcggcaat gccggcgacg
     5641 gcgcggtgga gattgtacca cttcgcatcg agagcgatat ccgccgccgc gccagatcca
     5701 cctccagcac gcgcacccgg actctctggc cgaccctgac cacgtggttc gggtcgcgca
     5761 cgaaccggtc cgcgagttgg ctcacgtgaa cgagcccatc ctgatgcacg ccgacatcga
     5821 cgaacgcccc aaatgccgtc acgttggtca ctatgcccgg cagtaccatg cccggcctta
     5881 cgtcctcgat cttcaccacg ccatcggcgt acgcgaaggc ctcgaacacc tgtctcgggt
     5941 cccttccggg cttggccagc tcggcaatga tgtcatgaag tgtcggcata cccaccgggc
     6001 cagacacata gcgcgacagg tcgatgcgtg cccgcatgga ctcctgggag agcagatcgg
     6061 ccactgtgca ccccgcgtcg cgcgccatag cgttgacaat gtgatagctc tccggatgca
     6121 cggcgctggc gtcgagtggg ttgtcgccgc cccgcaccct gagaaagccg gccgcctgct
     6181 cgaaggcctt cggacccagc cgcggcacag ccatcagctg ctcgcgcgac cggaacggtc
     6241 cgttctcgtt ccggtggttc acgatgttct gcgccaactg gggtcccagg cctgacacgt
     6301 aggtcagaag ctgtttgctc gccgtgttca catcgacgcc taccgcattg acgcaactga
     6361 ccaccacgtc atcgagggct cgtctcagcg ccgcttggtc gacatcgtgc tggtactgtc
     6421 cgactccgat ggactttgga tcgatcttga cgagctccgc caggggatcc atcagccgac
     6481 ggccgatgga cacggccccg cggaccgtca catcctggtc ggggaactcc tcgcgtgcga
     6541 cctccgaagc cgagtacacc gacgcgcccg attcgttcac caccacgacg gccacgctgt
     6601 ccggtaggcc cagtccgcga acgaaggctt cggtctcgcg gctcgccgtc ccgttgccga
     6661 tggcgatggc agcgggtctg aagcgctcca cgatgtcacg gagcttcggc cccgccttgg
     6721 cagcctgcgg gccgctgtcg tggatgtaga acacatcgtg gtgcacgagc ttgccctgct
     6781 catcaaggca caccaacttg cagcccgtcc gtaggcctgg atcgatggcg accacccgct
     6841 tctgtcccag cggcgctgtc aggagcagct cgcggacgtt ggacgcgaac acacggatgg
     6901 cctgctcatc ggcacgccgc ttgagttcgc cgcggatctc ggtctccatc gacggtgcca
     6961 acaggcgctt gtagccgtcc tcgacggcca gagccacctg ttcggacgcg gggttctggc
     7021 ccgtcacgaa ctgggccctc aactggcgta tcgccgcgtc ctcgggcggg gccaccgtca
     7081 accgcaggaa gccctcgttc tcgccgcgca tcatggccag gatccgatgc gatggcgcgt
     7141 tggcgatcgg ttcggtccac tcgaagtagt cgcggtactt ggccccctca tcggcccggt
     7201 ccctggccac gcgggagctg atgttcgact gggttgcgta gagccgccgc atgttgctcc
     7261 gagcctgttg atcctcactg acccgctccg cgatgatgtc ccgggcgccg gccaatgcct
     7321 cctccgccga cgccacaccc ttgtcttcat cgatgaaggt cgcggcctct ctcagcgggt
     7381 ccatggcctc cgtctgctcc aatagcgcca gcgccagggg ctcaagtccc ctctcgcgcg
     7441 ccacggtggc ccgcgtgcgc cgcttgggcc ggtagggcag gtagatgtcc tcgagaacgg
     7501 ccatggaggg ggcggcatcg acctgcgcgc gaagctcgct ggtcagcagt ccgcgttcgt
     7561 ccagcgactt gataatggcc gcgcgccgct tgtcgagttc ctcgagctga ccgagccgat
     7621 cgcggatggc cgcgatcgcc acctcgtcca gggctccagt cacctccttg cggtagcggg
     7681 caatgaatgg aacggtggcg ccttccgaca acagcgccgc tgtcgacgaa acctggtgag
     7741 gagccacacc cagctcccgc gcgatgatca gggaatgtcg ttctgtcata gatacctcgg
     7801 tccactatcg ggtcggagcg gcccacggag cgaccgcgga gtgccatata cgagggcgca
     7861 cccgcgccac ccttctccct cgtccgccgg aggagtgagc ccgtgcgcgc cggaagtccc
     7921 gcgacactcg cgctcacgga ggaggagccc catgcccgcg taccgccacc tggcgctgct
     7981 cgtcgtcacc gtcctggtcg cgcctctcgg tgcgcacgcg gccggcgaag aaggcgtcaa
//
LOCUS       DAOSPL010000015         7980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_9038_length_7980_cov_4.874826, whole genome shotgun sequence.
ACCESSION   DAOSPL010000015 DAOSPL010000000
VERSION     DAOSPL010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9038_length_7980_cov_4.874826"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="PLQ54_00590"
     CDS             <1..1263
                     /locus_tag="PLQ54_00590"
                     /inference="COORDINATES: protein motif:HMM:NF023168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOS91784.1"
                     /translation="PQARPCWLPLPGLLVEAARSRHIDLAALLDRLASAIQRADASGG
                     TGLLDFEERFYSQGRRVVPLRFVRELVARLRDEHSRPEGADVSSWLEFAHQGTGSRIH
                     LVLKQSALHKALSSVLVLNADACPDVHALRFAFPHFEVHDWPVEQHIHVTQFSNSSYS
                     KSVLMAHRSCREDVQRLVEFYRLVEDRVLVITHKSLLPEERVNEPDDDAFVGWWGKHH
                     RALNRFADIDALVVASYFCENLGVLRARVQALRDSREPRTHSDALSDAEPGRSSEYRI
                     YEGYRDDRGRGRARLIPVDADPDVQTAIDASWSGAVHQAIGRGRPLLRDPDNPLRVFL
                     LPAIPVEGLRVDDLQSTAAILGKDHPVTGLNREKRDEASDRVTEAIRILVAEGKRPTI
                     NAIYTRFGGRWNTIRRELERWRREHGMA"
     gene            1654..2961
                     /locus_tag="PLQ54_00595"
     CDS             1654..2961
                     /locus_tag="PLQ54_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91785.1"
                     /translation="MRTLRLSRAETEERAFAILVDRVRHAQEHVPFYRRRYQQAGVSW
                     HDLKTPDDLARFPVVTREDLVESWRDMIDERMPLESLRRYGSGGSTGVPVITYNTQEE
                     RYRTWAVEQLSFHIHGIPRGARIVRLWGAPQDMIKFRGLRGWAKQTFQNDSMLDAFHM
                     DPPIMATYVQRINALKPYAMLAYPEAADLLARYIEENGLAVHSPAAIVTSAGPLLPAY
                     RARIERVFRAPVMDRYGCREGGVISYQCPAQRGLHIFPFRTRVETVREGQPVGPGVDG
                     HILITPLMREAMPLIRYDVGDIGRLLENGCSCALPWPCMEVSVGRSSDVFVTSGGGLV
                     AGSGLTTFMANHEGLQLYQIEQATDRSVTVRLVTDERFTEASRDRIRTKLGNIFGPDI
                     PLNVVDVEDIPRTRSGKIRPIISQARIQLTRENAPDQLARGDE"
     gene            complement(2973..4472)
                     /locus_tag="PLQ54_00600"
     CDS             complement(2973..4472)
                     /locus_tag="PLQ54_00600"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOS91786.1"
                     /translation="MLRDLPRGQRILFVALGLLGGMGCGLAAGGYMGILFLVAPLVVF
                     VLSLVRPLAGVALLAVALPTYSLQIPGIEMSPFRAMGIVVFAGWILGALVRRDTLPRL
                     RPMDLCAFLYLMAAMLSAVVNGEAADAIETDVQLVVLYVMVIGVARTPKAARVLLAAV
                     LIGQVFNGGVAAYQRQTRNYSFLNPTVDLDAWGQRAPGLSDPNETGIKLAYTMPLALT
                     SMALSTTPLVVRGGLGVASCVMLFGLFGTASRGGVITFVAAVALWMIMWLLSSSSLPA
                     ARGLPNAPPSAASNRMRHIAIAVLLAVVLGGFYMKVVRNRLSEDFERRWNKTMTVDTA
                     NPLDDQAFTGRVGIWRRAMGMFAEKPFFGYGPRTGVKTDLEGPDDDTTAAHNTVLQVA
                     VQEGLVGSIPFHLAFWLSLHSLGRRRRELLEAGLELDRVYVSTLFVVILSLMFGALVG
                     TFAHDKDVWFLLGLASAMTAPGTQKGVEQAEARAESPFPTRRLRGRVTA"
     gene            4774..6084
                     /locus_tag="PLQ54_00605"
     CDS             4774..6084
                     /locus_tag="PLQ54_00605"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOS91787.1"
                     /translation="MITVLGAGYVGLVTGSCFADSGADVTFLDIDEDRVRKLRQGELP
                     IFEPGLQELFTRNTRAGRIRATTSPQEALAEARWVFITVGTPPREDGSTDLSYVEEAV
                     RTVGQNASNHVVIAIKSTVPVGTADRAREILAELGREDIAVVSNPEFLREGQAVRDFT
                     HPDRVVVGCRETEAGQSVVTLHKPYLGSNTHVFLMSNRSAEMTKYTANAFLATRISFI
                     NEIANLCEYLGADVEEVRLAAGADRRIGSHFFHPGVGYGGSCFPKDVRSIISMGSMHG
                     YPTRLLSSVHEVNENQKQRLAEKIRQRFGSDWSHLRVGVWGLAFKRDTDDTRESPAVV
                     LVDALLNAGAAVQVFDPAAMAAVGEHWEGGVVGCAGMYDAVVGVDALAVMTDWQEFRL
                     PNWAKVRKLMKGNVVFDGRNLYEPAVLEQEGLVHIGIGRGHQVS"
     gene            6115..7074
                     /locus_tag="PLQ54_00610"
     CDS             6115..7074
                     /locus_tag="PLQ54_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOS91788.1"
                     /translation="MRVLVTGGAGFIGSTLVDRLLRAGHSVVCLDNFDPYYDPALKRA
                     NLEEALQNPAFHLVEADLRDQIPVRATVGDARPDAIVHIAARAGVRPSLEDPHVYIDV
                     NIHGTLNLMDAAREFGKPKMIVASTSSVYGADTPVPFREDAALHSIVSPYAASKLGME
                     ALCHTYASVYGLRIAILRLFTVYGPRQRPDMAIHKFTRHIEQGLPIELYDHGQSYRDY
                     TYVDDIVSGFEAALDADGIEWGIFNLGGGRPTYLRALVAEIEKALGRTAQITEHPPAD
                     GDVPRTLADITRAREVLGYDPQVRVEEGVPRFVDWYRRHNRLH"
     gene            7162..7812
                     /locus_tag="PLQ54_00615"
     CDS             7162..7812
                     /locus_tag="PLQ54_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HOS91789.1"
                     /translation="MEVREAIRRARLEKGLSQKELADAIHVAQTNVSQWERGSTRPKA
                     RNLKRLAEVLGKPVDYFFGDQPLTPPNTDRSHEMPTPSDSVLVPLLGTLPLGSPLEAI
                     ETTDIFIRATPAEASQCDFAFRVVGNSMYPFFWDGDMVGVKLAEQATDRQIVVARLAS
                     GEATLKMYRQDPTSGQAWLEPLNKSHAPLRGEDFEIRGIVVWIRKPLPGGHIPSPV"
BASE COUNT         1488 a         2662 c         2488 g         1342 t
ORIGIN      
        1 ccccaggccc ggccctgttg gctgcccctc cccggcctgc ttgtcgaggc ggcccgcagc
       61 cgccatatcg acttggccgc gcttctggat cggttggcgt cggccatcca gcgggcggac
      121 gccagcggtg gcaccggcct cctggatttc gaggaacgct tctacagcca aggccggcgc
      181 gtcgtgccct tgcgattcgt gcgggagctt gtggcccggt tgcgcgatga gcactcgcgc
      241 ccggagggag ccgacgtctc ctcgtggctc gagttcgccc atcaaggcac cgggtcccgc
      301 atccatctgg tactcaagca gtccgccctg cacaaggccc tgtcctcggt gctggtgctc
      361 aatgccgatg cctgtcccga cgtccacgca ctccgctttg cctttcccca cttcgaggtg
      421 cacgactggc ccgtcgagca gcatatccac gtgacccagt tctcgaactc gagctactca
      481 aagagtgtgc tgatggccca tcgctcgtgc cgggaggatg tccaacggct cgtggagttc
      541 taccggctcg tggaggaccg cgtgctggtc atcacccaca aatccctgct tcccgaagag
      601 cgcgtcaatg agcccgacga cgatgccttc gtcggttggt gggggaaaca ccatcgggcc
      661 ctgaaccggt ttgccgacat cgatgccttg gtcgtggcca gctacttctg cgagaatctg
      721 ggggtcttgc gggctcgggt gcaggcgttg cgagactccc gggaaccccg cacccacagc
      781 gacgcgcttt cggacgcgga gcccgggcgg agttccgagt accgcatcta tgaaggctat
      841 cgggatgaca ggggtagggg acgcgcgcga ctgattcccg tggatgccga tcccgatgtc
      901 cagaccgcca tcgacgccag ttggtcggga gcggtgcacc aggccatcgg ccgcggccga
      961 cctctcttgc gggatcccga caacccgctg cgggtgttcc tgctgccggc gattcccgtg
     1021 gagggactcc gtgtcgacga tctacagtcc acggcagcca tcctcggcaa agaccatccc
     1081 gtcaccggcc tcaatcgaga gaagcgggac gaggcatcgg atcgcgtgac cgaggcgatc
     1141 cggattctgg tggctgaggg caaacgaccg accatcaatg ccatctacac gcgcttcggg
     1201 ggccgctgga acacaatccg ccgggagctg gagcggtggc gacgtgaaca cggaatggca
     1261 tgagagcgtg gtttccagag gaagaggacc ccttacaacg acttgttgtc gtgagttgcc
     1321 gaggaaagtg cccttacaac gggatgtaca taagattctg tacaagccgt tgtaaggggt
     1381 agttccctcc gattcgtaca acaacaagcc gttgtaaggg gtagattgtc cccggaaccg
     1441 ccggacagga cgtggcgggg acgggagctc cggccagcgc ctcgaaccgc tgggttgggc
     1501 cagccttcgt ctcgctcgcg cgtcgcgagg actccagcgt ttatctagct tacctgagag
     1561 ggagcttcca ccatgtcgtc tctccgcgcc gccctgggga gtgcttggct gaccgcgcgt
     1621 ggccataagg ggcccgcgta tctccgggag tacatgcgaa cacttcgcct cagccgggcg
     1681 gaaaccgagg agcgcgcctt cgccatcttg gtcgaccgcg tgcggcacgc ccaggagcat
     1741 gtgcccttct accgccgtcg ctaccagcag gccggcgtca gttggcacga cctcaagact
     1801 cccgatgatc tggcccggtt ccccgtcgtg acccgcgagg atctggtcga gagctggagg
     1861 gacatgattg atgagcggat gcccctcgaa tccttgcgtc gctatgggag cggcgggtcc
     1921 accggcgtcc cggtgatcac ctacaacacc caggaggaac gctatcgcac atgggccgtg
     1981 gagcaactga gcttccacat ccacggcatt ccccgcggcg cccgcatcgt gcggctctgg
     2041 ggcgcgcccc aggatatgat caagttccgc ggcctccggg gctgggcgaa acagactttc
     2101 cagaatgaca gcatgctgga tgccttccac atggacccgc ccatcatggc cacctacgtc
     2161 cagcgcatca acgccctcaa gccctatgcc atgttggcct atcccgaagc cgccgatctg
     2221 ctggcgcggt atatcgagga gaacggcctc gccgtgcact cgcccgcggc catcgtgacc
     2281 tctgcgggac ccctgttgcc agcctaccgt gcccgcatcg agcgggtgtt ccgggctccc
     2341 gtgatggacc ggtacggctg ccgggagggt ggggtcatct cctaccagtg ccctgcccaa
     2401 cgaggcctgc acatcttccc gttccgaacc cgggtcgaga cggtacgcga gggacagccc
     2461 gtggggcccg gagtcgatgg ccatatcctc atcacgccgc tcatgcgcga agccatgccg
     2521 ctgatccgtt acgacgtcgg cgatatcggc cgcctgctcg agaacggttg ctcgtgcgcc
     2581 ctaccgtggc cttgtatgga ggtgagtgtc gggcgctcca gcgatgtgtt tgtcacttcg
     2641 gggggcgggc tggtggctgg atcaggtctg accaccttca tggccaatca tgaaggcctc
     2701 cagctctatc agatcgagca ggccaccgac cgcagcgtga ccgttcgctt ggtcaccgat
     2761 gagcgtttca ccgaggcatc ccgtgaccgc attcgaacca aactggggaa catcttcggg
     2821 cccgacattc cgctgaacgt ggtggatgtc gaggacatcc cccgcacgcg atcgggcaag
     2881 attcgcccga ttatctccca ggcacggatc cagctcacac gggagaacgc ccccgaccag
     2941 ctcgcccgcg gagacgagta gtgggcatgc ccctacgccg tcactcgacc ccgcagtcgc
     3001 cgcgtgggga aagggctctc ggcgcgagcc tcagcctgtt cgaccccctt ttgtgtgccc
     3061 ggggccgtca tggcggaggc aaggcccaag aggaaccaca cgtccttgtc gtgggcgaag
     3121 gtacctacga gagcaccgaa catcagcgat aggatgacca cgaaaagggt gctgacgtac
     3181 actcgatcga gctcgagacc ggcttcgagc agttccctcc gccggcgccc gagactgtga
     3241 aggctgagcc agaacgccag atggaagggg atcgacccca ccagcccttc ctgcaccgca
     3301 acttggagta ccgtgttgtg ggctgcggtg gtgtcatcgt ccgggccctc caggtcggtc
     3361 ttcacaccgg ttcgcgggcc gtagccaaag aagggcttct cggcgaacat gcccatggcc
     3421 cgccgccaga tacccacccg gcccgtgaag gcctgatcgt ccagcggatt ggccgtgtcc
     3481 acggtcatgg tcttgttcca gcgtcgctca aagtcctccg aaagccgatt acggacgacc
     3541 ttcatataga acccgccgag caccaccgcc agcagcacgg cgatggcgat atggcgcatc
     3601 cgattgctgg ccgccgacgg cggagcgtta gggaggccgc gggccgcggg aagcgatgag
     3661 gacgagagca gccacatgat catccacaac gccacggccg ccacgaaggt gataacgccg
     3721 ccccgcgacg cagtaccgaa cagcccaaac agcatcacac agctcgccac gcccaggccg
     3781 ccacggacca cgaggggcgt ggtcgatagg gccatggagg tgagggccag gggcatcgta
     3841 taggccagct tgatccccgt ctcgttggga tcgctcaggc cgggggcgcg ctgaccccag
     3901 gcgtcgaggt ccactgtggg attgagaaag ctatagttgc gtgtctgccg ttgataggcg
     3961 gccacacccc cattgaagac ctgcccaatc agcacggcgg ccaacagcac ccgggcggcc
     4021 ttcggggtcc gtgccactcc gatcaccatc acgtacagca ccacgagttg aacatcggtc
     4081 tcgatggcat ccgcggcctc gccattgacc acggccgaga gcatggcagc catcagatac
     4141 aggaacgcgc agagatccat ggggcgaagc cgaggcaagg tgtcccggcg caccagcgcg
     4201 ccgaggatcc agccggcaaa taccacaatc cccatggccc ggaagggaga catctcgata
     4261 cccgggatct gcaggctgta ggtgggcaat gcgaccgcca ggagggccac tcccgccagg
     4321 gggcgcacga ggctcaggac gaacacgacc aggggagcta ccaggaacag gatccccatg
     4381 tacccgccgg cggccaagcc gcaacccata ccgcccagta ggcccagggc gacgaacagg
     4441 atccgttggc ctcgaggcag atcccggagc atctcatcga cccttcccac aacggtgcac
     4501 ccattgtagc gcaatccgcc caacgccccg cgcggcccac cgcgctggag acacccttcc
     4561 acacccgggc ggctccgaac cccatcgaac ccgcgaacat accgtgacct tagtccacca
     4621 gcggaatcat cgtgacgccc tcacaacgca tccgagacgc aacggcgtgg ggaagaatgt
     4681 tccgcggtat cctcggtacc gtggagaagc gtcgctcggc tcccgaggag cataagccat
     4741 tgatctctca gaaacctgtc aggaggaatc actgtgatca ccgtgctggg tgcgggatac
     4801 gtggggttgg tcaccggctc ctgtttcgcg gactcgggcg cggacgtgac gtttctggac
     4861 atcgatgagg accgcgtccg caaactcagg cagggagagc tccccatttt cgagcccggc
     4921 ctccaggagc ttttcacccg caacacacgc gccggacgga tccgggccac cacctcgccc
     4981 caggaggctt tggccgaggc acgctgggtc ttcatcaccg tgggaacacc gccgcgggaa
     5041 gacggctcca ccgatctgag ctatgtggag gaagccgtcc ggacggtggg gcagaacgcc
     5101 tcgaatcatg tggtcatcgc catcaagagc acggtacccg tgggcaccgc ggatcgcgca
     5161 cgagagatcc tggccgagct gggccgggag gacatcgccg tcgtctccaa ccccgagttc
     5221 ctccgggaag gccaagccgt gcgcgacttc acacaccccg atcgtgtcgt cgtcggttgc
     5281 cgggagaccg aagcgggcca gagcgtggtg acccttcaca agccctatct tggatccaac
     5341 acccatgtgt tcctcatgag caaccggtcc gcggaaatga ccaagtacac ggccaacgcc
     5401 ttcctggcaa cccggatttc cttcatcaac gagattgcca acctgtgcga gtatctcggg
     5461 gccgatgtgg aggaagtccg gcttgctgcg ggagcggatc gccgcatcgg ctcgcacttc
     5521 ttccatcccg gcgtcgggta cggaggctcg tgcttcccca aggatgtccg gtccatcatc
     5581 agcatgggat ccatgcacgg atacccgacc cgtctcctct cctcggtcca tgaagtgaac
     5641 gagaaccaga agcagcgact ggccgagaag atccggcaga ggttcggatc cgactggagc
     5701 cacctacgcg ttggggtctg gggtctggcg ttcaagcgcg acaccgatga cacccgcgag
     5761 tctcccgcgg tggtgctggt ggatgcgctt ctgaatgccg gggccgccgt acaggtcttc
     5821 gatccggccg caatggcggc ggtgggagag cactgggaag gcggcgtcgt gggttgcgcg
     5881 gggatgtacg atgccgtggt gggagtcgat gcgctggccg tcatgaccga ctggcaagag
     5941 ttccggctcc ccaactgggc caaggtgcgg aagctcatga agggcaacgt ggtgttcgac
     6001 ggccgcaatc tctatgagcc ggccgtgctc gaacaggagg gcctggtcca cattggcatt
     6061 gggcgcggcc atcaggtctc ctgacatact gggggcgaga aagggcggca tcggatgaga
     6121 gtcctcgtga ccggcggcgc tggtttcatc ggttcgaccc tggtcgaccg cttgctcagg
     6181 gccgggcatt cggtcgtctg cctggacaac ttcgacccct actacgaccc cgccctcaaa
     6241 cgggcgaacc tcgaagaagc gctccagaat cccgccttcc acctcgtcga agccgacctg
     6301 cgcgaccaga tcccagtacg agccacggtg ggagatgccc ggcccgatgc catcgtccac
     6361 atcgccgcgc gcgcgggcgt gcgcccctct ctcgaggatc cacacgtgta tatcgatgtg
     6421 aacatccatg gcaccctgaa cctgatggat gccgcccggg agttcggcaa gcccaagatg
     6481 atcgtggcct ccacctcatc ggtctatggc gccgacaccc ccgtgccctt ccgcgaagat
     6541 gcggcgctcc attccatcgt ttccccctat gcggcaagca agctcggcat ggaggcgctc
     6601 tgccatacct atgcgtccgt ctacggcctc cggatcgcca tactccggct gttcacggtc
     6661 tacggccccc ggcaacggcc cgacatggca atccacaagt tcacgcgaca catcgagcag
     6721 gggctcccta ttgagctgta cgaccatggc cagagctatc gcgattacac ctatgtcgat
     6781 gacatcgtgt ccgggttcga ggccgctctc gacgccgacg gcatcgagtg gggtatcttc
     6841 aacttgggag ggggccggcc cacatatctc cgcgccctgg tggcggagat cgagaaggcc
     6901 ctcggccgca cggctcagat caccgaacat cctcccgccg acggcgacgt gccgcgcacc
     6961 ttggccgaca tcactcgcgc gcgcgaagtg ctcggatatg acccccaggt gagagttgag
     7021 gagggcgtgc cccgtttcgt ggactggtac cggcggcaca accgcctcca ttagcttcgg
     7081 gaaggaggaa cgacattcgg cgggaaacgt cttcccgccc cacggttgcg tgaaggggcg
     7141 agggaaacgg aggcgagggg cttggaagtc agagaagcca tccgtcgcgc ccgcctggag
     7201 aaaggcctct cccaaaaaga gctggccgac gccatccatg tggctcagac caatgtctca
     7261 caatgggagc gtggcagcac gcgccccaaa gcccggaacc tcaaacgctt ggccgaggta
     7321 ctgggcaaac ccgtggacta cttcttcggg gatcagcccc tgaccccccc caacaccgac
     7381 cgttcccacg agatgcccac tcccagcgac agtgtgctgg tcccccttct gggtaccctc
     7441 cccctgggca gccccctgga agccatcgag accaccgaca tcttcatccg tgctaccccg
     7501 gcggaagcct cccagtgcga cttcgccttc cgggtcgtgg ggaacagcat gtaccccttc
     7561 ttctgggatg gagacatggt gggcgtcaag ctagctgaac aagccaccga ccggcagatc
     7621 gtggtcgccc gcctggcttc cggtgaggcc accttgaaga tgtaccgcca ggacccgaca
     7681 tcgggacagg cctggctcga acccctcaac aaaagccatg cgccgctccg cggcgaggat
     7741 ttcgagatcc gagggatcgt cgtgtggatt cgaaaacccc tccccggggg gcacattccc
     7801 agcccggtct aagacggagc cctggatccc gcaagcgctc catgccgcct gcctctcccc
     7861 tgagatacgc ctccgcatcg atggggcaga accagccccc cgtcctggag gttccattca
     7921 cgttcgaagg gttggacggc accaggacgt gagagggagt cggtgatgcc ccaagactcc
//
LOCUS       DAOSPL010000016         7946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_9090_length_7946_cov_3.354581, whole genome shotgun sequence.
ACCESSION   DAOSPL010000016 DAOSPL010000000
VERSION     DAOSPL010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7946
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9090_length_7946_cov_3.354581"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PLQ54_00620"
     CDS             complement(<1..694)
                     /locus_tag="PLQ54_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOS91790.1"
                     /translation="MRACLLASLIAAFAGGPPAAALSVADCGAVGDGIADDTAAFAQA
                     LSTGSAWVPPGHYVVGELAIPDGARLAGEGPASVLTLRSGAKFVLRPGARCVIEDLSI
                     VGNPDSGAQSGDEGLIHGVRADGWAARDLWVSGAGICGVLLDHCNDVRIQDCQLERLR
                     RAVSIVFSHDVMVEGNVVRDCSEHGLMFWGNWQWERMECRNISFIGNRVVRGGGGAIW
                     GTGGVGVVATGNI"
     gene            complement(811..2346)
                     /locus_tag="PLQ54_00625"
     CDS             complement(811..2346)
                     /locus_tag="PLQ54_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOS91791.1"
                     /translation="MLVAAGMPSAQAPKPGDVVFATGFDTEAERGAWPAAPFAEWAEG
                     VDGSQGLRVHVEAGQESAAHMVSIPVDLEPFRGCKLFLECMAKADNVSKPLQPYLGAK
                     FMLHYLSETSGPFWQNQNDVYGTFDWKKLSFVAPIAPDATGGQISLGLQESSGTVSFD
                     DIRISVYKTAPPARPQPPADPGPVFRGHNLPRLRGVMSPNAPTDEDLRVLGQEWNANV
                     MRRQITRNWGQPGTDRDLAEYDQWFEGKLAELDRDLEACQRYGIQVVIDMHSPPGGRY
                     ENSDLAIFNEPLYQDHFVELWEKMARRYRGHPAVWGYDLVNEPVQNLPSPPGVADYLG
                     TQVRAARAIRAIDPDTPIFIEACEWDSAQGFRELEPVDVPHVIYQVHMYVPGEFTHQG
                     VFSKVTGIRYPGEIGGVMWDKEQLRKVLAPVREFQLAYNVHIYCGEFSAIRWAPGAAD
                     YLRDCIELFEEYGWDWTYHAYREWDGWSLEHGPDPENHEPTAEPSDRMQVLLGWFARN
                     VKP"
     gene            2523..5861
                     /locus_tag="PLQ54_00630"
     CDS             2523..5861
                     /locus_tag="PLQ54_00630"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase family protein"
                     /protein_id="HOS91792.1"
                     /translation="MSLGGKAMRVGMLAGLLALLGVGVGPAMADHELWRIGSADGDTA
                     DLALGPDGYAQFAGDPLFIVGVDDPQTRWPYVHPGPHDTWAGSRAHAFSVVFGLAAAP
                     AAPARLEVQCVDTHGSGPPRLDITINGRGSGQLTCPRGAGDASVKGDPKAGKPWRFSV
                     DIPADALRAGNNVLRIANVAGSWLLYDSLALVTAGELETKPVEPSFVAHGFESPPVLV
                     RGDGELKQLVHIRGTRIGPATTARVRVGGQVAVEAKVSPGDTVVEVAVNRVKREQTAA
                     VSISVDGSPEQTGSVVLRPVREWTVCIIHHTHLDIGYTHTQEDVEKLQMKYLDQVLEL
                     IDQTAGNPPEARFTWMPEGLWAVESWLRTADTEKREAFFAAVRAGRIGLDGLYGNALT
                     ALYTDEELFALIDYARRLRREHELPLDSAMISDVPGLTWGMVPALAQSGIRYLSDGPN
                     PGHRIGLSRVWDDRPVWWVGPSGQDRILLWQSGLGYAHFHGRRISEAAILEYLSRLEQ
                     QRFPYDIAQVRYNIGGDNGYPDTELPGFVMAWNERYAYPRLMLTTNSGMFHMLEDRYG
                     DQLPAISGDFTPYWEDGAASTAAATSAERAADELLVQAGALYAMLAPKHYPRERVHEA
                     WRQAVLYDEHTWGAHNSISEPDSPFAVQQDAYKQRFATTARDMARQILDDALAGIRAD
                     GPVVALEVLNPSSWPRTQLVVLPKEWSLPGDRIRRGERVVPSQRLTTGELAFLAEGVP
                     PYGSLGYTVEPGPATALGHARAQALTLENGPVSLSVNPKTGAIASLTVRGLPGNLVDT
                     TAESGLNAYVYIAGRDPKSRQGVSDVRVRIVEPGPLVASLQVESKAAGAYGLSTEIRL
                     VSGLPHVEIANALDKAKVREKEGVHFAFPFAVPDATPRIETPWAIYRSDVDQLEGSCR
                     NYLTAQRFADVSNGRLGVTLVTVDAPLVQVGEIRTDVANQFGASDSWIRRLPASSTLY
                     SYIMNNYWETNYKADQSGVVPFRYAIRPYAGLFDGAAASRFGAETCQPLVVVPVRRPT
                     DRASQLRVSPPGVIATSLRPSEDDSLLLRLYAASGKPESASVEWGDGSAPEVSIERPD
                     GSFGRATWPVRLPAYGVVTLKLER"
     gene            complement(6292..>7946)
                     /locus_tag="PLQ54_00635"
     CDS             complement(6292..>7946)
                     /locus_tag="PLQ54_00635"
                     /inference="COORDINATES: protein motif:HMM:NF025681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HOS91793.1"
                     /translation="WYPAIPVPKMILEVPFAGDVEEGMVLESAAGLAARAALAGRSRT
                     LVWERTGNDGYERWYAGYCRDHRPERIAVPLDELVARLRDLRIVRGYALYRADTTTRP
                     LHEAGPMDVSANVATSLAGKLAAIVVSERLEARFRALGLPCLFDARGLTEEKCLARYG
                     RVLSRRGMGLADPKTRNARSLMIALDMAVVSGQGAAYEQALARCEPDTPVLCWGCGTE
                     DGITIPSSRWGLFQTATNWCHNLTVLASEAIAGSGLVSRRPPARRVPNPPSDARHYVC
                     LTITDGDNIQWLMGNFVGGTEGRYYYANPRRGAVPFTWGLPVPGLALLSPRTLGEVLE
                     TATRRDDFVMYSGGGYFYPDLYGAARPEARALELHAERLRGYCDRTGIRILAFNFQDW
                     DSEAAQHACRVFAQHIPGLLGILAFQYYPYSAGEGRILWVDGPNGDRVPVVSCRYTVW
                     AETGRPRDSTPAAIAAELNQLPVEEGGTFSWVLVHAWSYFRHSPGSNDPKAEEPGPSP
                     DVADPALQRGYDPALWTVERLAPHVVPVGAEELLLRIRARGE"
BASE COUNT         1300 a         2834 c         2621 g         1191 t
ORIGIN      
        1 cgatgttgcc tgtggcgaca acgcccacac cgcccgtgcc ccagatcgct cctccgcctc
       61 ctcgcacgac ccgattgccg atgaagctaa tattacggca ctccatacgc tcccactgcc
      121 agttgcccca gaacatgagc ccgtgctcgg agcagtcgcg gaccacgttg ccctccacca
      181 tcacatcgtg cgagaacacg atcgataccg cgcgtcggag ccgttcgagc tggcagtcct
      241 ggatgcgcac atcgttgcag tggtcgagca agaccccaca gatgcccgcg ccactcaccc
      301 acagatcccg ggcagcccac ccatcggcgc gcacgccgtg gatcaacccc tcatcaccgc
      361 tctgcgcgcc ggaatcgggg ttacccacaa tggatagatc ctcgatcacg caacgcgcgc
      421 cgggccggag cacgaacttg gcgcccgagc gaagcgtcag caccgacgcc ggcccctcgc
      481 cggcgagacg ggcgccgtcg gggatcgcca actcgccgac gacgtagtgg cccggcggca
      541 cccacgcgga gccggtggac agcgcctggg cgaacgctgc cgtatcgtcg gcaatgccat
      601 cgcccaccgc accgcagtcc gcgacggaca gggctgcggc aggggggcca cccgcgaacg
      661 cggcgatcag gctggcgagc aggcaagcgc gcatgaatgg cacatcctcg ctctgatgcg
      721 atgctcaggc atgaacctgg cgacgcgcgg ccgccaaatg gagtccgggg gaccattccc
      781 cacccggtct gaggcggagc ccccgggcac tcagggcttg acgttccgcg cgaaccagcc
      841 gagcagcacc tgcatgcggt ccgatggctc ggcggttggc tcgtggttct ccgggtcggg
      901 cccgtgctcg aggctccacc cgtcccactc gcgatacgcg tggtaagtcc agtcccagcc
      961 gtactcctcg aacagctcga tgcagtcgcg gagatagtcc gcggcgccgg gcgcccaccg
     1021 gatggcgctg aactcgccac agtagatgtg cacgttgtag gcgagttgaa actcgcgcac
     1081 cggcgcgagg accttgcgga gctgctcctt atcccacatc acgccaccga tctcgccggg
     1141 gtaccggatg ccggtgacct tgctgaacac gccctggtgc gtgaactcgc cagggacgta
     1201 catgtgaacc tggtagatga catgggggac atccacgggc tccagttcgc gaaacccctg
     1261 ggccgaatcc cactcgcacg cctcaataaa gatgggagta tcaggatcga tggctcggat
     1321 cgcccgggca gcgcgaacct gggtgccgag gtaatcggcg acgcccggcg gcgaggggag
     1381 attctgcacc ggctcgttga caaggtcata gccccacacc gcagggtggc cccggtaacg
     1441 ccgggccatc ttctcccaca gttcgacgaa gtggtcctgg tagagcggct cgttgaagat
     1501 ggccaagtcg ctgttctcgt accgcccacc cggcggcgag tgcatgtcga tgacaacctg
     1561 gatgccgtag cgctggcaag cctcgaggtc gcggtcgagc tcggccagct taccctcgaa
     1621 ccactggtcg tactcggcca ggtctcggtc cgtgcccggc tggccccagt tccgagtgat
     1681 ctgccgccgc atcacattgg cgttccactc ctggcccagc acgcgcaagt cctcatcggt
     1741 cggcgcattg ggcgacatga cgccacgcag ccgcggcagg ttgtggcccc ggaagaccgg
     1801 cccgggatcc gccggcggct gcggccgggc cggcggcgcg gtcttgtaga cgctgatgcg
     1861 gatgtcgtcg aatgacaccg tgcctgagct ctcctgcagg ccgagggaga tctgcccgcc
     1921 tgtcgcgtct ggagcgatgg gggcgacgaa gctgagcttc ttccagtcga acgtgccgta
     1981 gacgtcgttc tggttctgcc agaatggccc actggtctcc gagaggtaat gcagcatgaa
     2041 cttggcgccc agataaggct gcagaggctt cgacacattg tcggccttgg ccatgcattc
     2101 gagaaagagc ttgcatccgc ggaagggctc tagatcaacg ggtatgctga ccatatgggc
     2161 cgcgctctcc tggccggcct ccacgtgaac acgcagcccc tggctcccgt cgacgccctc
     2221 ggcccactcg gcgaacggcg ccgcgggcca ggcaccgcgc tcggcctcgg tgtcgaatcc
     2281 ggtggcgaag accacatcgc caggcttcgg tgcctgggcg gacggcatcc cggccgcgac
     2341 gagcaatcca agtcccacga ggcagcaagc tgatgaagcg ttcgtgagca tagccatccc
     2401 cctgaccgca atcggcccgc ccttcgacgc agccacccgc tctgcctctc ccgtcagccc
     2461 gacacagggc ggcctcgccc cgctggggaa cacaaccacg gctgatatgg tcttggaggg
     2521 tgatgagttt gggcggcaag gcgatgaggg tcggtatgct ggctgggctt ctagcgctgc
     2581 tgggcgtggg cgtaggcccg gcgatggccg atcacgagct ctggcggatc ggctccgccg
     2641 acggcgacac ggccgatctg gccctcggac ctgacggcta tgcccagttc gccggtgatc
     2701 cgctgttcat cgtcggcgtc gacgacccac agacccgatg gccctacgtc catcccggcc
     2761 cgcacgacac gtgggcgggc agccgggccc acgcgttctc cgtggtcttt ggcctagcgg
     2821 ccgcgccggc agccccggcg cggcttgagg tccagtgcgt cgacacgcac ggcagcgggc
     2881 cgccacggct cgacatcacc atcaacggcc ggggctcggg gcagttgact tgcccacggg
     2941 gcgcaggcga cgcctccgtc aagggtgacc cgaaggccgg caagccctgg cgcttctcag
     3001 tcgacatccc ggccgatgcc ctgcgcgccg ggaacaacgt gctgcggatc gccaacgtcg
     3061 ccggcagttg gctgctctac gacagcctgg cgctggtcac cgcaggcgag ctggagacga
     3121 aaccagtgga gccgagcttc gtggcccatg gtttcgagtc gcctcccgtg ctcgtgcgcg
     3181 gcgatggcga gctcaagcag ctcgtccaca tccgcgggac gcgcatcggc ccggcgacca
     3241 cggcccgcgt ccgcgtcggc ggccaggtgg cggtcgaggc caaggtgagc cccggagaca
     3301 ccgttgtgga ggtcgccgtc aaccgcgtga agcgcgagca gacggcagcc gtgtccatct
     3361 ccgtcgacgg ctcgcctgaa cagacgggct cagtggtctt gcggccggtg cgcgagtgga
     3421 ccgtctgcat catccatcac acccacctcg acatcggcta cacgcacacg caggaagatg
     3481 tcgagaagct ccagatgaag tacctcgacc aggtgctcga gctgatcgac cagacggccg
     3541 gcaacccgcc tgaggcgcgc ttcacctgga tgcccgaggg cctgtgggcg gtcgagagtt
     3601 ggctcaggac ggccgatacg gagaagcggg aggccttctt cgccgcggtc cgcgccggcc
     3661 gtatcggcct cgacggcctc tacggcaacg cactcacggc gctctacacc gacgaggaac
     3721 tcttcgctct catcgactac gcgcggcgac tgcggcgcga gcatgagctg ccgctcgact
     3781 ctgccatgat cagcgatgtc ccggggctca cctgggggat ggtgcccgcg ctggcccaga
     3841 gcggcatccg ctacctgtcc gacggcccca accccgggca ccgcatcggc ctctcgcgcg
     3901 tgtgggatga ccgcccggtc tggtgggtcg ggccgagcgg acaggaccgc attctgctct
     3961 ggcagtcggg cctcggctac gcccacttcc acgggaggcg gatcagtgag gcggccatac
     4021 tcgagtacct ctcgcggctc gaacagcagc ggttcccgta cgacatcgcc caggtgcgct
     4081 acaacatcgg tggcgacaac ggctaccccg ataccgagct gcccggcttc gtcatggcct
     4141 ggaatgagcg ctatgcctac ccgagactga tgctcaccac caacagcggc atgttccaca
     4201 tgctcgagga ccgctacggc gaccagctcc ccgccatcag cggcgacttc acaccctact
     4261 gggaggacgg tgcggcgtcg accgccgcgg ccacctcggc cgagcgcgcg gccgacgagc
     4321 tcctcgtcca ggctggcgcc ctctacgcca tgctggcgcc gaagcactac ccaagagagc
     4381 gcgtccacga ggcctggcgg caggccgtgc tctacgacga acacacctgg ggcgcccaca
     4441 acagcatctc cgaacccgac tcacccttcg cagttcagca agacgcctac aagcagcggt
     4501 tcgccacgac ggcccgcgac atggcgcgcc agatcctcga cgatgcactg gccggcatcc
     4561 gcgccgatgg cccggtggtt gcgcttgagg tgctcaaccc ctcgtcatgg cctcgcacgc
     4621 agcttgtggt actgccgaag gagtggagcc tgcccggcga ccgcatccgg cgcggtgagc
     4681 gtgtcgtacc atcgcagcga ctcaccacgg gcgagctggc cttcctggcc gaaggtgtgc
     4741 cgccctatgg ctcgctcggc tacacggtcg aaccgggccc ggcgactgcc ttgggccacg
     4801 cccgagccca ggcgctcacc ctcgagaacg gacccgtctc cctgtcggtg aacccgaaga
     4861 cgggcgccat cgcctccctc acggtacggg gtctgcccgg caacctggtc gacaccacgg
     4921 ccgagagcgg actcaacgcc tacgtctaca tcgcggggcg tgacccgaag agccgccagg
     4981 gggtctccga tgtgcgcgtc cgcatcgtcg aacccggccc gctggtggca tcactgcagg
     5041 tcgagtcgaa ggccgcgggc gcctatgggc tcagcaccga gattcgcttg gtgagcggac
     5101 tgccacacgt cgagatcgcc aacgctctcg acaaggcgaa ggtgcgggag aaggagggcg
     5161 tgcacttcgc ctttcccttc gcggtgcccg acgcgacgcc gaggatcgag acgccctggg
     5221 ccatctaccg atctgatgtc gaccagctgg agggctcgtg ccggaactac ctgaccgccc
     5281 agcggttcgc cgatgtgagc aacggccggc tcggggtgac gctggtcacc gtcgatgcgc
     5341 cgttagtgca agtaggcgag atccgcaccg atgtggccaa ccagtttggc gcctcggatt
     5401 cctggatccg acgtctgccg gcgtcgtcga cgctgtactc atacatcatg aacaactact
     5461 gggagaccaa ctacaaggcc gaccaatcgg gcgtggtgcc cttccgctac gccatccgac
     5521 cctacgcggg tctgttcgac ggcgcggcgg cctcgcgatt cggggccgag acgtgccagc
     5581 cgctcgtagt cgtgccagtg aggcggccaa ctgaccgcgc ttcgcagctc cgggtcagcc
     5641 cgcccggtgt catcgcgacc tcgctgaggc cgagtgagga cgactccctg ctcctccggc
     5701 tctacgcggc ctccggcaag cccgagagcg cctcagtcga gtggggcgac ggctctgcgc
     5761 ccgaggtcag catcgagcgc cccgacggct ccttcggccg agccacctgg cccgtgcggc
     5821 tcccggccta cggggtggtg acgctgaagc tggagcggtg agcgagggcc agcgactctt
     5881 ttcgtgccga tactggctat cctgtcactc gccgcgggct caagcgcgca gcaggctgcg
     5941 cgctgcccct gacccccata ggcgctaacc cagagctcgg atggcgctgc aggcacaccg
     6001 ggacggcgta tctgggtctg acggaggggc cacaggggga ggaccgtccg gcgaagccgg
     6061 atggtgggcc cccaatgggc cctggtcggc cgccagtgct ctgcaactgc gctctccggc
     6121 tcatcgaggg ccgtcgagcg ccgggccgat tagggtccca ccagcctgcg gctggtcccc
     6181 cctatcaggc ccctccgcaa gaccaggcag cacgctcgcc gcgacctccg cgatgcccag
     6241 ccgcccgacc aacggcccca agttagcgcc tatgcccctg atcgctgccc ctcactcccc
     6301 ccgcgctcgg atgcgcagca gcagctcctc cgcgccgacc ggcacgacgt gtggcgcgag
     6361 gcgctcgacc gtccagagcg ccgggtcgta gccgcgctgg agggcgggat cggccacgtc
     6421 aggcgacgga cccggctcct cggccttcgg gtcattgctg cccggcgagt ggcggaagta
     6481 ggaccacgca tgcacgagca cccacgagaa cgtgccaccc tcctcgacgg ggagctggtt
     6541 cagctccgcc gcgatggcgg ctggtgtcga gtcccgcggg cggcccgtct ccgcccacac
     6601 cgtgtagcgg caagagacga ccggcacccg gtcgccgttg gggccgtcca cccacaggat
     6661 gcgcccctcg ccggccgagt aggggtaata ctggaaggcc agaatgccca gcagtcccgg
     6721 aatgtgctgg gcgaagaccc ggcacgcatg ctgggcggcc tccgagtccc aatcctggaa
     6781 gttgaacgcg aggatgcgga tgcccgtacg gtcgcagtat ccccgcagcc gctcggcgtg
     6841 cagctccagg gcacgggcct ctggccgtgc agctccgtac aggtcggggt agaagtagcc
     6901 cccgccggag tacatcacga agtcgtcccg ccgcgtggcc gtctcgagga cttcgcccag
     6961 cgtgcgcggc gacaggagcg ccaggccagg cacaggcagg ccccaggtga acggcacggc
     7021 gccgcgccgg gggttggcgt agtagtaccg gccctccgtc ccgccgacga agtttcccat
     7081 caaccactgg atgttgtctc catcggtgat cgtgaggcac acgtagtgcc gcgcgtctga
     7141 gggcgggttg ggaacccgtc ttgcgggagg gcggcgtgaa acgagtcctg accccgctat
     7201 cgcctccgag gccagcacgg tcaggttgtg gcaccagttg gtcgcggtct ggaacagccc
     7261 ccagcgcgac gaagggatgg tgatgccgtc ctcggtcccg cagccccagc acagcaccgg
     7321 cgtgtcgggc tcgcaccgcg caagggcctg ctcgtacgcc gcgccttggc cggagacaac
     7381 ggccatgtcc agggcgatca tcagcgaccg cgcattgcgc gtcttggggt cggccaggcc
     7441 catgccccgg cgcgagagca cgcgcccata gcgcgccagg cacttctcct cggtgaggcc
     7501 ccgcgcgtca aacaggcacg gcagcccgag ggcgcggaac cgcgcctcca gcctctccga
     7561 caccacgatg gccgctagct tccccgccag cgacgtcgcc acgttggcgc tgacgtccat
     7621 gggcccagct tcgtgcagcg gtcgtgtggt cgtgtcggcg cgatagagcg cgtacccgcg
     7681 gacgatgcgc aggtcgcgca ggcgcgccac gagttcgtcc agcggtacgg cgatgcgctc
     7741 gggccggtga tcgcggcaat agcccgcata ccagcgctcg tacccgtcgt tgcccgtccg
     7801 ctcccacacg agggtacgcg accgcccggc caaggcggca cgcgccgcca gccccgcagc
     7861 cgactcgagc accatgccct cctcgacatc gccggcgaag ggcacttcga ggatcatctt
     7921 cggcactggg atggccgggt accacg
//
LOCUS       DAOSPL010000017         7808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_9299_length_7808_cov_3.234361, whole genome shotgun sequence.
ACCESSION   DAOSPL010000017 DAOSPL010000000
VERSION     DAOSPL010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9299_length_7808_cov_3.234361"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="PLQ54_00640"
     CDS             <1..880
                     /locus_tag="PLQ54_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91794.1"
                     /translation="PDGSREALPHAASLVLGLAGFAFLVGFSMRAGATLWYAVSFFVI
                     HFGLGFAVTRIRAEMGLPAHDLHHAGPGQILYRVLGGFIIPKPSQTVGQIYYWFNRAY
                     RSYASPHQAEGFKLAERTGTSVRALFPVMALAVALGSLASFWAILHLGYQDGFAAKVA
                     EPKVSLIFGREPYDELVAHLNAPERPSLPAVWAMVVGFVTVFGLMHLKTRVLWWPFHP
                     VGFAVSASWAMQHLWCPLFLAWGAKWTLTRYGGHSAFRQAVPFAFGLILGDLIGGSFW
                     TIYGIAKHVATYSIWV"
     gene            886..5904
                     /locus_tag="PLQ54_00645"
     CDS             886..5904
                     /locus_tag="PLQ54_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOS91795.1"
                     /translation="MQMRMGPRTVLWAGLALCLAAGAARAATDIAQFVRPVRPAAIEK
                     TIEDLSALGSRVAGYPGADQAARYVLNQFRTLGLRNVRTQAFPVTVPVTRHAWVRVRA
                     GGVDRRLDIYPLWPNRVRTCQTPPAGLTGPLVDGKSGEFTDLNGHEIAGSILIVDFNS
                     ETRWFNGPLLGAQAVLFPEPDSVSRGEAESKFLSPPASMPRFWVKKDDARWLRELVAR
                     EDNVSATVCADVRWERRTAYNILGEVLGTNAEYAKERLVLESYYDSMSVVPDLAPGAE
                     SACGIAALLEIARCFAQSPPERSILFLATSAHFEALGGTREFLRLHREAGWSTPYNAS
                     IPAADSSVQVRVHLNPEGEKPLPGTGDTGDANPAAGTKYPTVSLRLGAGAMIKPGQDT
                     LTIRSKLADAPFSATISLTDPAGWEREDEFGRWVKAEQAPAAVDNRLSGDAIQYRYMT
                     EWSGAPELLGSRDFKVTPALDFEEASAPGDDAKPPKITAFISIDLSSRSDLMCVFYKG
                     HFYNMNEAQQWKYSDFGKSCTTFAGQVESALGISASAHFADAINSITGSNWKSYLPCR
                     LAMDFEQAAVASEVAVGFATANDMRALVDTPMDVAESVDTGSVTLQTRLLAGLLHAML
                     AKGTTGDKSDPADLGGLDFARKTKPLTLDADLWCVIDGNIVYFDPTQDYYPNEPVPGA
                     IAFARGPAKTSAGVRGDILQMVGHDGRFTFHGVPMSKIAYYGFGMEGFLLRPEDGEII
                     MAPDLGQSGAQAFPIDQVPDAQQKTIRVVVFPCKAMSLYDLVDQRQFMFLNTVSVLNA
                     VSNASPFSWGMSLPEPSRQWQSYLEPVAVVYGKSGEQLKFGFGLSVLGNRFLLLNGTR
                     DDPMGKGYDLGQNPILYPLPRRVAEDMWYLNESRIETLRGYGIKNKRMEELHAGAWEA
                     LAQADAARKALRYDEFFRYLREALGYETKVYPDVMKTTMDVVKGVIFYLALLLPFSFF
                     CERLFFASMTMGRQILGCAVFFLAIFCVLWVVHPAFQITRAPLIVLLAFVMMTLTLLV
                     TYIIVTKFDQRMRFLKQQVTGLRETDVGRLSASSAAFSLGVANMRRRRLRSTLTSVTL
                     ILLTFTLMSFTSVVSETRYNRIKVDDHGRYYGLQIRDRQWNPISLPALRFMRNEYGDR
                     YPVAPRAWYLSEMVGNISRVGLTYEGKRIEAAALVGLTPAEESITMPGESLVAGRWFS
                     DQDQGLTCILPAPLAKIFGLTEADIDRAEITILGSRCKLIGIIDPLRFQQCVDIDGEP
                     LTPVDYQMMAQKQRTGDAAAQDQVLNEYIHYNPNQVVLVPFQFALDAGASLRSIAVNI
                     GDPDAIDEEVESLLKRVELNMYTRGKRSLLVSAVATSGLQAASQGILIPLLIASLIVL
                     NTMLGAVYERLQEIGIFSSLGLAPMHIAFLFVAEALVYAVLGAVMGYLVGQMVAKGVV
                     AFGWFPELSLNYSSVAGAGTAVVVMITVLASTLYPARMAGRLATPGIDRRWKLVDPQE
                     GVFEVELPFSSSTDQVLGLNAFLYEYLGAHVEYSTGKFSADNVDLVREGDVDAGPRYH
                     LASTVWIAPYDLGVRERFTLVSAPVEDQEGAYGFVAIIRREDGDEGAWIRLTRNFLTV
                     LRQQFLLWRTFDAVSRAEYAVKGYTILGEVPPAGLVAFVEAAKTRDEDESAAPAAAEE
                     APVG"
     gene            5953..>7808
                     /locus_tag="PLQ54_00650"
     CDS             5953..>7808
                     /locus_tag="PLQ54_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HOS91796.1"
                     /translation="MAVRDQELELYRGLMEAPKEYAEGFTKRSIIGAFFVGLIMMPGS
                     IYLGLLAGQGLGPAAEWVTIILFTEAARRSFTVLRRQEIYILYYMAGALTGGIGLVAL
                     SGGPFAGLIWNQYLVQSDAALGFGIRDQIPYWVVPSADSPALVERTFFHPHWFVPILL
                     LVLGNILGRLNWFGLGYVLFRITSDVERLPFPMAPIAAQAATALAEVTAKTEGWRWHL
                     FSIGAVIGIAFGLPYVGIPAFTGSVLTRPVQLIPIPFYDMTLRLERTFPAVPFALSTD
                     LGAAIVGFVLPFWVVIGSALATLASLIIHPWLARNQLMPTWSPGMDYIQTSFATSVDF
                     WTSFGIGTSAAVAVIGIVSILRSASRMKRETSGRGTFQPPPGRGDFPIWIGIALFAAS
                     TLTYILLCRLWLVPKFPLVFLVFFGFIQSPVESYINARMIGLTGQYIGIPMVREASFI
                     LSRYKGVDIWFAPIPMSNHGGQAQNLRVVELTGTKITSIIKAELFMLPLILICSLCFW
                     QYMWKLAPIPSTAYPYAQKMWHLQALRSCLWMTATAQNNEMFMRAINWKYIVAGGASA
                     LGSYGVLSVLQLPVMLVWGFVRGLGGNPMDAIPQLFGALLSQFYFVRRYGMR"
BASE COUNT         1278 a         2552 c         2497 g         1481 t
ORIGIN      
        1 ccccgacggc agccgtgagg ctctgccgca tgcggcgagt ctcgtgctgg gcctggccgg
       61 gtttgcgttc ctcgtcgggt tctccatgcg cgcgggggcc accctgtggt acgcggtgtc
      121 cttcttcgtg atccacttcg gactaggctt cgcggtgacc cgcatccggg cggagatggg
      181 gcttcccgcc catgacctcc accatgccgg gccgggacag atcctgtacc gcgtgctcgg
      241 tgggttcatc atccccaaac cgagccagac ggtggggcaa atctactatt ggttcaaccg
      301 ggcataccgg tcctacgcgt caccccacca ggcagaggga ttcaagctgg ccgagagaac
      361 aggcacgagc gtgcgggcct tgttcccggt gatggcgttg gccgtggcgc tcggatcgct
      421 cgcatcgttc tgggcgatcc tgcacctggg gtaccaggat ggcttcgccg ccaaggtggc
      481 cgagccgaag gtcagtctca ttttcggccg tgagccatac gacgaactgg tggcccatct
      541 gaacgcgccc gaacggccga gtctgcccgc cgtgtgggcc atggtggttg ggttcgtcac
      601 ggtgtttggg ctgatgcacc tgaagacccg tgtgctctgg tggcccttcc atccggtcgg
      661 gttcgccgtc tcggcgagct gggcgatgca gcacttgtgg tgccccctgt tcctggcatg
      721 gggggccaag tggactctga cgcgctacgg cgggcactcg gcgtttcgcc aggccgtacc
      781 gttcgcgttt ggtttgatac tgggggactt gatcggcggc agcttctgga ccatctacgg
      841 catcgccaag catgtcgcca cctattcgat ctgggtgtag ggacgatgca gatgcgcatg
      901 ggccctcgaa cggtgttgtg ggcgggcttg gccctgtgtc ttgccgcggg agcggcgcgc
      961 gccgcgacgg atatcgctca attcgttcga ccggtgcgac ccgctgcgat tgagaagacc
     1021 atcgaggacc tgtccgcctt gggtagtcgc gtggctggct acccgggtgc agatcaggcc
     1081 gcgcggtacg tcctgaacca gttccgaacg ctggggctca ggaacgttcg aacccaagcg
     1141 ttcccggtga cggtacccgt gactcggcac gcgtgggtcc gcgtccgggc gggaggtgtc
     1201 gatcgccgcc tcgacatcta tcccctctgg cccaaccgcg tgcgcacctg ccagactccc
     1261 cccgctgggc tcaccggacc cctggtcgac ggaaagtccg gcgagttcac ggacctcaac
     1321 gggcacgaga tcgccggcag catcctgatc gtggacttca acagcgagac ccgctggttc
     1381 aacggaccgc tactgggtgc ccaggcggtg ctcttcccag agcccgactc cgtcagccgg
     1441 ggcgaggcgg aatccaagtt cctctcacct ccggccagca tgccgcggtt ctgggtgaaa
     1501 aaggacgacg cgcgctggct ccgcgagttg gttgcgcgcg aggacaacgt gtccgccacc
     1561 gtatgcgcgg atgtgcgctg ggagcggcgc accgcctaca acattctggg cgaggtcctg
     1621 ggcaccaacg cggagtatgc gaaggagcgc ctggtcctgg agtcttacta cgactcgatg
     1681 tccgtcgtgc ccgatctggc acccggcgcc gagtccgcct gcggcatcgc cgccctgctc
     1741 gagatcgcgc gatgcttcgc gcagagcccg cccgaacggt cgatcctgtt cctcgccacg
     1801 tcggcccact tcgaggcact gggaggcacc cgggagttcc tccgactcca ccgcgaggcc
     1861 ggatggagca cgccctacaa tgcaagcatt cccgcggccg actcgtcggt ccaggtgcga
     1921 gtgcacctga accccgaggg cgagaaaccc ctaccgggga ccggcgacac tggcgacgcg
     1981 aaccccgccg cgggcaccaa gtatcccacc gtaagcctcc gcctgggcgc cggggccatg
     2041 atcaagccgg gccaggacac actcaccatt cggagcaaac tggccgacgc gccattctcg
     2101 gccaccatct cgctgaccga tcccgccggc tgggagcgcg aggacgagtt tggcaggtgg
     2161 gtgaaagcag agcaggctcc cgccgccgtc gataaccgcc tcagcggcga cgccatccag
     2221 taccgctaca tgaccgagtg gtccggtgcc ccagagctcc tgggaagccg ggacttcaag
     2281 gtcactccgg ccctggattt cgaggaggcc tccgccccgg gcgatgacgc caagcccccc
     2341 aagatcacgg ccttcatcag catcgacctc agcagccgca gtgacctgat gtgcgtgttc
     2401 tacaagggcc acttctacaa catgaacgag gcccagcagt ggaagtactc cgacttcggg
     2461 aaatcgtgta cgacctttgc gggccaggtg gagagtgccc tgggcatctc agccagtgcg
     2521 cacttcgcgg acgccatcaa ctcgatcacc ggcagcaact ggaagtcgta tctgccgtgc
     2581 cggctggcca tggacttcga gcaggccgcc gtggcttccg aagtggcggt cggttttgcc
     2641 acggccaacg atatgcgggc gctggtcgac acacccatgg atgtggcgga gtcggtcgac
     2701 accggcagtg tgaccctcca gacgcgtctg ctggccggtc tgctgcacgc catgctcgct
     2761 aaaggcacca cgggggacaa gtcggacccg gcggacttgg gcggcctcga cttcgcccgc
     2821 aagaccaagc cgctgaccct tgacgcggat ctgtggtgcg tgatcgatgg caacatcgtc
     2881 tactttgacc ccacccagga ctactacccc aacgaacccg tgccgggggc tatcgccttc
     2941 gcccgaggcc ccgccaaaac cagtgcgggc gtgcgtggcg acatcctgca gatggtgggg
     3001 cacgatggcc ggttcacctt ccacggcgtc cccatgagca agattgccta ctacggcttc
     3061 ggcatggagg gattcctcct ccggcccgaa gacggcgaga tcatcatggc ccccgacctc
     3121 ggccagtcgg gtgctcaggc ctttccgatc gaccaggtgc ccgatgccca gcagaagacc
     3181 atccgcgtgg tggtgttccc ctgcaaggcc atgagcctct atgacctggt tgaccagcgt
     3241 cagttcatgt tcctgaacac ggtgtcggtg ctcaacgccg tatccaacgc ctcacccttc
     3301 tcgtggggca tgagcctgcc cgaaccgtcg cgtcagtggc agtcgtacct ggagcccgtg
     3361 gccgtcgtct atggcaagag cggcgagcaa ctgaagttcg gcttcggact ctctgtactc
     3421 gggaaccggt ttctgctgct gaacgggacg cgggacgacc cgatgggcaa ggggtacgac
     3481 ctgggccaga acccgatcct gtaccctctt ccccggcggg tggccgagga catgtggtac
     3541 ctcaacgagt cccgcatcga gacgcttcga gggtacggga tcaagaacaa gcgtatggaa
     3601 gagctccacg ccggcgcgtg ggaagccctc gctcaggccg atgcggctcg aaaggcactg
     3661 cgctacgacg agttcttccg atacctgcgc gaggcgctgg gctacgagac caaagtctac
     3721 cccgatgtca tgaagaccac catggacgtg gtcaagggcg tcatcttcta cctggcactt
     3781 ctcctgccgt tctcgttctt ctgcgagcgg ctctttttcg catccatgac catgggccga
     3841 cagatcctcg gctgcgccgt cttcttcctg gccatcttct gcgtcctgtg ggtcgtccac
     3901 ccggcattcc agatcactcg agctccactg atcgtgctgt tggccttcgt catgatgacc
     3961 ctcacgttgc tcgtgacgta catcatcgtg accaagttcg accagcgaat gcggttcctc
     4021 aagcagcaag tcacggggct gcgcgaaacc gatgtgggac ggctgagcgc gtccagcgcg
     4081 gcattctcgc tcggtgtggc caacatgcgc cggcgccggc tccggagcac cctgaccagc
     4141 gtgaccctga tccttctcac cttcacgctc atgtcattca catcggtcgt atccgagacg
     4201 cggtataacc ggatcaaggt cgatgaccac gggcggtact acggtctgca gatccgcgac
     4261 cggcaatgga accccatctc actgccggcc ctgcgcttca tgcgcaatga atacggtgat
     4321 cggtaccccg ttgccccgag ggcctggtac ctctcggaga tggtcgggaa catcagccga
     4381 gttgggctca cctacgaggg caaacgcatc gaggcggccg ctctggtggg ccttactccg
     4441 gccgaggaaa gcatcaccat gccaggggag agcttggtcg ctggccgctg gttcagcgat
     4501 caggatcagg gtctgacgtg catcctgccc gcccccctgg cgaagatctt cggcctcacc
     4561 gaagccgaca tcgaccgggc ggagatcacg atcctgggct cccgatgtaa gctcatcggc
     4621 atcattgacc cgctgaggtt tcagcagtgc gtcgacatcg acggggagcc tttgacgccg
     4681 gtcgactacc agatgatggc gcagaaacag cgaacagggg atgccgcggc gcaggaccag
     4741 gtcctcaacg agtacatcca ctacaacccg aaccaggtgg tactggtccc gttccaattc
     4801 gccctggacg ccggagcctc cctgcggtcc atcgccgtca acatcggcga cccggacgcc
     4861 atcgatgaag aggtcgagtc tctgctcaag cgggtggagc tgaacatgta cacccggggc
     4921 aagcggagcc tgctcgtcag cgcggtggcg accagcggac tccaagcggc cagtcagggc
     4981 atcctgatcc cactcctgat cgcgtcactg atcgtgctca acaccatgct gggggccgtg
     5041 tatgagaggt tgcaggagat cggcatcttc tccagcctgg gcttggcacc gatgcacatt
     5101 gcgttcctgt tcgtggcgga ggcgctggtg tacgccgtcc tcggtgccgt catgggctac
     5161 ctggtggggc agatggtggc caagggcgtg gtagccttcg gctggttccc cgagctgagc
     5221 ctcaactact cgtcggtggc gggggccggg acggcggtcg tcgtcatgat cacggtgctg
     5281 gcctcgacgc tttacccggc ccgaatggct ggccgcctgg cgacgcccgg catcgaccgc
     5341 cggtggaagt tggtcgaccc acaagagggc gtgttcgagg tcgaactgcc gttctcgtct
     5401 tcgaccgatc aggtacttgg attgaacgcg ttcctctatg agtacttggg ggcgcatgtc
     5461 gagtactcga ccgggaagtt ctcggccgac aatgtcgatc tggtccgcga gggtgacgtg
     5521 gacgccgggc cgcggtatca cctggcatcc acggtctgga tcgctccgta cgacctgggg
     5581 gtccgcgagc ggttcacgct ggtgtccgcg ccggttgagg atcaggaggg ggcctacggc
     5641 ttcgtcgcca tcatccgccg cgaggacggg gacgagggcg catggatccg cctgacgcgc
     5701 aacttcctga ctgtgttgcg gcagcagttc ctgttgtggc ggacctttga cgccgtgtcc
     5761 cgggccgagt atgccgtcaa aggctacacc atcctgggcg aggtcccgcc agcggggctt
     5821 gtcgcgttcg tcgaggccgc caagacgcgc gacgaagacg aatcggctgc cccggcggcg
     5881 gccgaggagg ccccggttgg ctgagtcgcg agtcggacca ctggccacat cctgtaggca
     5941 ttcggagtcg ctatggccgt aagagaccaa gaacttgagc tctatcgggg gctgatggag
     6001 gcccccaagg agtacgccga aggattcacc aagcggtcca tcatcggggc cttctttgtg
     6061 ggcctgatca tgatgccggg gtcgatctac ctcggcctcc tggcgggcca gggcctgggt
     6121 ccggcggccg agtgggtcac gatcatcctg ttcaccgaag cggcccggcg gtcgttcacg
     6181 gtgttgcggc ggcaggagat ctacatcctg tactacatgg ccggggccct gacaggcggc
     6241 attggcctgg tggccctttc gggcgggccc ttcgcgggcc tgatctggaa tcagtatctg
     6301 gtgcagtcgg atgcggccct cgggttcggg atccgcgacc aaatccccta ctgggtcgtc
     6361 ccgtccgccg actcgccggc actcgtcgaa cggaccttct tccaccccca ttggttcgtg
     6421 cccatcctgc tcctggttct ggggaacatc ctgggccggc tcaactggtt cgggctgggg
     6481 tatgtgctgt tccgcatcac gtcggacgtc gaacgcctcc ccttccccat ggcacccatc
     6541 gcggcgcagg cggccacggc gctggcggag gtgacggcca agaccgaagg ctggcgctgg
     6601 catctgttct ccatcggcgc ggtcatcggc atcgcctttg gcctgcccta tgtgggcatc
     6661 cccgccttca ccgggtccgt actgacgcgg cccgtgcagc tcatccccat ccctttctat
     6721 gacatgacgc ttcgcctgga gcgtaccttc cctgccgtgc ccttcgcctt gagcacggac
     6781 ttgggcgccg ccatcgtcgg gttcgtgctg ccgttctggg tggtcattgg ctccgcgctg
     6841 gcgacactgg cgtcgctgat catccacccc tggctggcgc gaaaccagct catgcccaca
     6901 tggtcgcccg ggatggacta catccagaca tcctttgcga ccagcgtcga cttctggacc
     6961 tcgttcggca tcggcaccag cgcggcggtg gcggtcatcg gcatcgtgag cattctgcgg
     7021 tcggcgagcc gcatgaagcg ggaaacctcc gggcgtggga cgttccagcc gccgccgggc
     7081 cggggtgatt tcccgatctg gatcggcatc gccctcttcg ccgcgtcgac gctgacgtat
     7141 atccttctgt gccgactctg gctggtgccc aagttccccc tggtcttcct ggtgttcttc
     7201 ggcttcatcc agtcgccggt ggagtcgtac atcaatgcac ggatgatcgg gctgaccggc
     7261 cagtacatcg gcatcccgat ggtgcgggag gccagcttta tcctcagccg ctacaaagga
     7321 gtcgacatct ggttcgcccc gatcccgatg agcaatcatg gtggccaggc acagaacctg
     7381 cgcgtggtcg agctcacggg caccaagatc accagcatca tcaaggcaga gctgttcatg
     7441 ctcccgctga tactgatctg cagcctgtgc ttctggcagt acatgtggaa gctggctccc
     7501 atcccttcca ccgcctaccc gtatgcgcag aagatgtggc acctgcaagc gctgcgcagc
     7561 tgtctgtgga tgacggcgac cgcgcagaac aacgagatgt tcatgcgagc catcaactgg
     7621 aagtacatcg tcgcgggcgg agcctcggca ttgggctcct acggcgtgct ctccgttctc
     7681 cagctgcccg tgatgctggt gtgggggttc gtacgaggtc tgggcggtaa cccgatggat
     7741 gccatcccgc agctgttcgg cgccctcctg agccagttct actttgtccg gcgctacggc
     7801 atgaggga
//
LOCUS       DAOSPL010000018         7790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_9337_length_7790_cov_2.789528, whole genome shotgun sequence.
ACCESSION   DAOSPL010000018 DAOSPL010000000
VERSION     DAOSPL010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7790
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9337_length_7790_cov_2.789528"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="PLQ54_00655"
     CDS             complement(<1..996)
                     /locus_tag="PLQ54_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS91797.1"
                     /translation="MRTTRRRHWALLALTASITLAGAHAQPDDGVYADLCVYGGTAAG
                     VVAAITMARLGGSAAIVEPGQHLGGLTSSGLGQTDVGNAAAIGGISREFYGRILRYYT
                     DRYGADSPQVKACNGGFWFEPHVAEVVLGQMVKEQRVPVYFGERLDRVGVERGRVRFI
                     RTTAGTRFRARMFIDATYEGDLMAKAGVTYTVGREPVDQYGEPHNGVQFGQPYHNFKA
                     PVDPYVVPGDPSSGLLPGVSGEDPGVHGQGDHRVQAYNFRMCLTTRPGNRRPFPKPKG
                     YDPNRYTLLLRYIEAGVWDALQLAALLPNWKTDTNNNGGFSTDNIGMNYEWPEGDA"
     gene            1151..2035
                     /locus_tag="PLQ54_00660"
     CDS             1151..2035
                     /locus_tag="PLQ54_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS91798.1"
                     /translation="MFQLAAFTDEISQDFEHAVAVCQEYGVPAVEIRSVWQKKGPQDL
                     LDGELARMLDILSAARMTTACIASPFLKCDLGDEDAYRAHLGILRRCCQVARMMGTDI
                     IRGFTFWRVPDLTEAQYRQILAHFEEPCRILSGEGMRLGIENEHSCNVGTGLELRKFL
                     DDLRACGYPGCERVGAIWDPANQVFANVPETPYPDGYEAVRADMIHMHVKDAQWEGTQ
                     CVCTAVGDGAVDYEGQFQALTDDGYMGYCSLETHWRPQALTEEEVKRPGGAAYSESGE
                     YASRTCLENISRMLAQLG"
     gene            2123..4360
                     /locus_tag="PLQ54_00665"
     CDS             2123..4360
                     /locus_tag="PLQ54_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOS91799.1"
                     /translation="MSGSEDLLRGLNPQQREAVTHGDGPLLVFAGAGSGKTRVLTHRI
                     AYLIAARGAKPHSILAVTFTNKAAGEMRERVHRLTGYSGGMWVGTFHGMCARILRQSG
                     EAIGVSPDFTIYDQSDQEAVMKAVVAELGWDTKDQANSPRSQLERISRAKNELLSPDE
                     FARRASDYADQRTAKAYRLYQGRLDAARALDFDDLIRRVVDLLVQQPAVLEEWRQRFQ
                     HVLIDEYQDINFAQYSFVMRLAAVHRNICAVGDDDQSIYRWRGADVKLILQFEKDFPN
                     AHVVKLEQNYRSTQRILDAAHAVISHNRARADKRLWTDRGEGQKLRAYEAIDERQEAL
                     WIAGEVKQWAGKEGAYSDHAVLYRTNAQSRVLEEVFRDQGIPYRLVGGVRFYDRKEVK
                     DLLAYLRLLYNPFDIVSLRRTINTPARGIGDAAVERLEAAAADDGVTPFQSLMRLESY
                     KERLARSYAPLKSYADLLRSLLAAAEATPVPELITRIIVDSGYEASLKKERSAEADGR
                     LENLYELATAAEKYAADSEDRSLGGFLEHVALVSDVDSLDQSSNAVTLMTLHAAKGLE
                     FSRVFIAGFEEYVFPHQRAMIDEQELAEERRLCYVGMTRAEDELVLTHARVRSMFGQT
                     RCNPPSRFLAEVPHTILEWQRASSAFRVDLDLGLAAHGAEPRRRLDLTDVVARHKRPG
                     AQGTKTGTEPGMADATYSPGDRVRHRTYGDGVVVSAEPGFVQVAFERQGVRKLALAFA
                     PLTKV"
     gene            4434..6515
                     /gene="ligA"
                     /locus_tag="PLQ54_00670"
     CDS             4434..6515
                     /gene="ligA"
                     /locus_tag="PLQ54_00670"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOS91800.1"
                     /translation="MGQPDDGGAAARIEQLRRLIEYHNHRYYVLDAPEIADHEYDALF
                     RELQDLEASHPELVTPNSPTQRVGAPPVESFAPAPHAFPMLSLANAFDANDLREFDAR
                     CKRMLGMGPEAIIEYTVEAKIDGLGISLTYEQGHFVRGATRGDGTTGEDVSANLRTIR
                     AIPTILPDGLGLPSSFEVRGEVFLSKTEFARINAERGIEGLPLFANPRNAAAGTIRQL
                     DSSVAARRRLQARFYDIRSDGRIPASTQLEVVELLRSAHFPTNDPTQMALGVEEVVRL
                     CGEAEHTHSALDFAVDGMVVKVNSLALQAELGQVSRSPRWAIAYKFPPERARTRVLRI
                     VAQVGRTGAVTPVAEMEPVFLDGSTVSRATLHNEDEVRRKDVRIGDFVWIQKAGDIIP
                     EVVEVDVAARSGDEQEFAMPAECPVCGGAVVREEGEAVARCSNPLCVAKLKTGLQHFA
                     SRRAMDIDGLGPAIIEQLVDRGLVRDFADLYQLTHEQLAGLDRMAGKSAANLLAAIEA
                     SKRTSLARLVFALGIRHVGEHVADGLATHMGSLERLRSATEDELSSIHEIGPQIAASV
                     AGFFADPRAQALLDRLVAAGLTWEEARSTSNMEDAFIAGKTFVFTGKLEHMTREEAEA
                     LVRSRGGRAASSVSAKTHCVVAGPGAGSKLDKARELGIEVMSEDEFLSKLGCPPDADN
                     PTGPHQQSLAF"
     gene            6532..>7790
                     /locus_tag="PLQ54_00675"
     CDS             6532..>7790
                     /locus_tag="PLQ54_00675"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOS91801.1"
                     /translation="MPRTDITIRGAREHNLKNLTLTIPRDKLVVFTGVSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLDQMEKPNVELIEGLSPAIAIEQKTASRNPRSTVAT
                     VTEIHDYMRVLWARCGVQHCHQCGKPISSQTPDQMVDRLMALEEGTRLQILAPVVLDR
                     KGEYADVFEEARRDGYARARVDGQVIDLSEEIHLSKRRKHRIEIVVDRLVIRPQSQAR
                     LAESMETALKLGDGMVLVDVVDSEELLFSQHAACPDCGISFGPLEPQMFSFNAPQGMC
                     PECSGMGTTLEMDETLVVTDPDRSLSEGAIELLGNPETQHTKHVLEALHRHFGVDIDR
                     PFRELPLEHRRIVLQGSPDTVRFRYRTRNGHTWEYSRTYDGVLSQAQRRFINSQTDEE
                     REELGQFLSNTPCPACKGGRLKPQSAAV"
BASE COUNT         1337 a         2497 c         2637 g         1319 t
ORIGIN      
        1 ggcgtcgccc tcgggccact cgtagttcat cccgatgttg tcggtcgaga acccgccgtt
       61 gttgttcgtg tcggtcttcc agttcgggag cagggccgcg agctgtaggg catcccacac
      121 gccggcctcg atataccgga gcagcagcgt gtagcggttg gggtcgtacc ccttgggttt
      181 ggggaacggc cgacggttac cgggccttgt tgtgaggcac atgcgaaagt tgtaggcctg
      241 gacgcggtgg tcgccctgtc cgtgaacacc ggggtcttcg cccgagacgc cgggcaggag
      301 gccggatgat gggtcgcccg gcacgacata ggggtccact ggcgccttga agttgtggta
      361 cggctgcccg aactgaacgc cgttgtgcgg ttcgccatac tggtccaccg gttcgcggcc
      421 cacggtgtat gtgaccccgg ccttggccat gaggtcgccc tcgtaggtcg cgtcgatgaa
      481 catgcgagcg cggaaccggg tccctgcggt tgtcctgatg aagcgcaccc ggcctcgctc
      541 aacgcccacg cggtcgagtc tctccccgaa gtagaccggg acgcgctgct ccttcaccat
      601 ctgaccgagg accacttcgg ccacatgggg ctcgaaccag aacccgccgt tgcatgcctt
      661 cacctggggc gagtcggcac cgtaccggtc cgtgtagtac cggaggatcc gaccgtagaa
      721 ctcgcgcgag atcccaccga tggcggccgc attgccaaca tcggtctggc cgagcccact
      781 cgatgtgagc ccacccagat gctgaccggg ctcgacgatg gcggccgatc cgcccagccg
      841 cgccatggtg atggcagcga ccacgccggc ggcggttccc ccgtacacgc acagatcggc
      901 gtatacgcca tcatccggct gagcatgcgc gcccgccagc gtgatcgatg ccgtgagggc
      961 caggagtgcc cagtgtctcc tccgtgtggt tcgcaccgct tcgtgccttt ccgtagtcgc
     1021 aatcctggcc gccgggttcg gtgccgccgc gtcggccccc tccctggggg gtgaggtccg
     1081 gcgcacggca ggaccgccat tgggggtggg cgaatacgcg tcccaccagc ccgagggagg
     1141 cgcatgtgcc atgttccagc tcgccgcgtt caccgatgag atctctcagg acttcgagca
     1201 cgcggtcgcg gtgtgccagg agtatggtgt tccggcagtg gagatccgat cggtctggca
     1261 gaagaagggt ccacaggatc tgctggacgg cgagttggcc cggatgctcg acatcttgag
     1321 cgccgcgcgc atgaccacgg cgtgcatcgc ctcgcctttt ctgaagtgcg acttgggtga
     1381 cgaggacgca taccgggccc atctgggcat cctccggcgc tgctgccagg tcgcgcgcat
     1441 gatgggcacg gacatcatcc gcgggttcac cttctggcgg gtgcccgatc tgaccgaggc
     1501 gcagtaccgc cagatcctgg cccactttga ggagccctgc cggatcctct cgggcgaggg
     1561 catgagactg ggcatcgaga acgagcactc gtgcaatgtg ggcacgggcc tcgaactccg
     1621 gaagttcctg gacgacctgc gtgcatgcgg ttaccctggg tgtgagcgtg tgggtgccat
     1681 ctgggacccg gccaatcagg tcttcgccaa cgtgccggag acgccatatc ctgatgggta
     1741 tgaggccgtc cgggcggata tgatccatat gcacgtcaag gacgcccaat gggagggcac
     1801 gcagtgtgtc tgcacggccg tcggcgatgg tgcggtggac tatgaggggc agttccaggc
     1861 cctcaccgac gacggctaca tgggctactg ctcactggag acccactggc ggccccaggc
     1921 gctgacggag gaagaggtca agcggcctgg tggtgctgcc tactccgagt ccggcgagta
     1981 cgcgtcccgc acatgcctcg agaacatcag ccgaatgctg gctcagctgg gctagtgccc
     2041 gggcgagccg gaacgtggtg tcgccgcctc tccgtgtgtc tcgcggaggg gctcttcgct
     2101 acgggcaggg agccaaccgc cattgtcagg ctcagaggat ctgctccggg gactcaatcc
     2161 ccagcagcgt gaagccgtaa cccacgggga cggcccgctg ctggtcttcg cgggggcggg
     2221 cagcgggaag actcgagtgc tcacacaccg catcgcctac ctcatcgcgg cgcgcggcgc
     2281 caagccgcac tccattctgg cggtcacttt caccaacaaa gcggccggtg agatgcggga
     2341 gcgcgtgcac cgtctcaccg gctacagcgg cggcatgtgg gtcggtacct tccacggcat
     2401 gtgtgcccgc atcctgcggc agtccgggga ggccatcggc gtcagccccg acttcaccat
     2461 ctacgaccaa tcggaccaag aggccgtcat gaaggccgtg gtggccgagt tgggctggga
     2521 taccaaggat caggcgaaca gccctcgctc ccagctcgag cgcatcagcc gggccaagaa
     2581 cgagctgctg tcacccgacg agttcgcacg gcgtgcgtcg gactacgccg accagcgaac
     2641 ggccaaggca tatcggctgt accagggccg gctcgatgcc gcgcgggcgc tggacttcga
     2701 tgacctgata cgtcgcgtcg tggatctcct cgtgcaacag ccggcggtgc tcgaggagtg
     2761 gaggcagcgt tttcagcatg tgctcataga tgaatatcaa gatatcaact tcgcgcaata
     2821 ctccttcgtg atgcgactgg cggcggtgca ccggaacatc tgtgcggtgg gcgacgatga
     2881 ccagagcatc tatcgctggc gcggagctga cgtgaagctc atcctccagt tcgagaagga
     2941 cttccccaac gcgcatgtgg tgaagctcga gcagaactac cgatcgaccc agcgcattct
     3001 cgatgccgct cacgcggtga tctcgcacaa ccgcgcccgg gccgataagc ggctctggac
     3061 cgaccggggc gagggtcaga agctgcgggc ctatgaggcc atcgacgagc ggcaggaagc
     3121 cctctggatc gcgggcgagg tgaagcagtg ggccggcaag gagggcgcct acagcgacca
     3181 cgcggtcctc taccgcacca atgcccagtc gcgcgtcctg gaggaggtgt tccgcgacca
     3241 gggcatcccc taccgcctcg tgggaggcgt gcggttctat gatcgcaagg aggtcaagga
     3301 cctactggct tacctccggc tcttgtacaa cccgttcgac atcgtcagtc tgcgacggac
     3361 cattaacacg ccggcgcggg gcatcggcga tgccgcggtc gagcgtctgg aggcggctgc
     3421 ggctgacgat ggggtgacac ccttccagag tctgatgcgg ctcgagagct acaaggagcg
     3481 cctggcgcgg agctacgccc ccctcaagag ctatgccgac ctgctcagga gtctcctcgc
     3541 ggcggcggag gcgacgcccg ttcccgagct gatcacccgc atcatcgtgg actcgggcta
     3601 tgaggcctcg ctcaagaagg agcgatccgc cgaggctgac ggacggctcg agaatctgta
     3661 cgagctggcc acggcggcgg agaagtacgc ggcggacagc gaagaccgga gcctcggggg
     3721 cttcctcgag cacgtggcgc ttgtgtcgga cgtggacagc ctggaccagt cgagcaatgc
     3781 cgtgacgctg atgacactgc acgcggccaa gggcctcgag ttcagccggg tcttcatcgc
     3841 ggggttcgag gagtacgtgt tcccgcatca gcgggcgatg atcgacgagc aggagctggc
     3901 cgaagagcga cgcctgtgtt acgtggggat gacgcgggcc gaggatgagc tggtgctcac
     3961 tcatgcccgc gtgcggtcca tgttcggcca aacgcggtgc aacccgccct cgcggttcct
     4021 cgctgaggtg ccgcacacga tcctggagtg gcaacgggcc tccagcgcgt tccgagtcga
     4081 cctcgacctt gggctggcgg cccacggcgc ggagccgcgg cgccgactcg acctgaccga
     4141 tgtggtggcc cggcacaaga ggcccggcgc gcaggggacg aaaacgggga ctgagccggg
     4201 catggctgac gccacttata gccccggcga tcgcgtccgg catcgcacct atggcgacgg
     4261 cgtcgtggtc tctgcagagc ccggcttcgt gcaggtggca tttgagcggc agggcgttcg
     4321 caagctggcg ctggcgttcg caccgctcac caaggtgtag acttggggcc aaggtaccgg
     4381 cccgtgccct tcacgccgga tgaatgcctt cggaccgcag ggggccaatg cacatgggtc
     4441 agcccgacga tgggggtgcc gcggcgcgca tcgagcagtt gcgtcggctc atcgagtacc
     4501 acaaccaccg ctactacgtg ctggacgccc ccgagattgc cgaccatgag tacgatgcgc
     4561 ttttccgtga gctgcaggac ctcgaggcca gccaccccga gctggtgacg ccgaactcgc
     4621 ccacccagcg agtgggcgct ccgcccgtcg agtcgtttgc ccccgcgcct cacgccttcc
     4681 ccatgctgag tctggccaac gccttcgatg ccaacgatct ccgggagttc gacgcccgct
     4741 gcaaacgcat gctcggcatg ggcccggagg cgatcatcga gtacaccgtc gaggccaaga
     4801 tcgacggcct cgggatctcg ctcacctatg agcaaggtca cttcgtgcgc ggggcaacgc
     4861 ggggagacgg cactaccggt gaggatgtct ccgcgaacct ccgcaccatc cgtgcgatcc
     4921 ccacgatctt gcccgacggc cttgggctcc cgtcgtcgtt cgaggttcgc ggcgaggtct
     4981 tcctctcaaa gacggagttc gcgcgcatca acgccgagcg agggatcgag gggcttccgc
     5041 tcttcgcgaa cccacgcaac gcggcggcgg ggaccatccg ccagttggac tcctccgtcg
     5101 cggcacgccg acgcttgcag gcacgcttct acgacatccg cagcgatggc aggattcccg
     5161 cgtcgaccca gctcgaggtg gtcgaactcc tcaggtcggc gcacttcccc accaacgacc
     5221 cgacccagat ggcgctgggc gtggaggaag tggtgcgcct ctgtggtgag gcagagcata
     5281 cccactcggc cctggacttt gcggtggatg gcatggtagt caaggtcaac tcgctggctc
     5341 tccaggccga gctcgggcag gtgtcgcgca gcccaaggtg ggccattgct tataagttcc
     5401 ctccggagcg tgcccggact cgggtcttgc ggatcgtcgc ccaggtcggg cgcaccggtg
     5461 ccgtgacacc ggtggccgag atggagcccg tcttcctgga tggttccacg gtgtcccgcg
     5521 cgacgctgca taatgaggat gaggtccgcc gcaaggacgt ccggatcggc gatttcgtct
     5581 ggatccagaa agcaggcgac atcatccccg aagtcgtcga ggtggatgtg gccgcgcgtt
     5641 caggcgatga gcaggagttc gccatgccgg ccgagtgccc ggtctgtggc ggggccgtgg
     5701 tccgtgagga gggggaggcc gtcgcgcggt gcagcaatcc gttgtgcgtg gccaagctga
     5761 agactgggct gcagcacttt gcctcacggc gggccatgga cattgacggg ctggggccag
     5821 ccatcatcga gcagttggtc gaccggggcc tggttcgcga ctttgccgat ctgtaccagt
     5881 tgacccatga gcaactcgcc ggactcgacc gcatggccgg gaagtccgcg gcgaacttgc
     5941 tggcggcgat cgaggccagc aagcgcacat ccttggcgcg cctcgtgttc gcgctgggga
     6001 tccggcacgt gggtgaacat gtggcggacg ggctggcgac acacatgggg tcactcgagc
     6061 gactgcgctc ggccaccgag gacgagctgt cgtccatcca tgagattggt ccccagatcg
     6121 cggcgagcgt ggccgggttc ttcgccgacc cgcgtgccca ggcgcttctc gatcggctgg
     6181 tggccgcggg cctgacttgg gaagaagcgc ggtccacgag caacatggag gacgcgttca
     6241 tcgcgggcaa gaccttcgtg ttcaccggca agctcgagca catgacgcgc gaggaggcgg
     6301 aggcactggt gcggagccgg ggaggccgtg cggcctcatc ggtcagtgcc aagactcatt
     6361 gcgtcgtcgc tggccccggc gccgggtcca agctcgacaa ggcgcgcgag ctcggcatcg
     6421 aggtcatgtc cgaggacgag ttcctgtcga agctgggctg cccgcccgat gcagacaacc
     6481 ccacagggcc gcatcagcag tcgctggcct tctgacccag agagacatgc catgcccaga
     6541 accgacatca cgatccgagg tgcccgtgag cacaacctca agaacctgac actgacaatc
     6601 cctcgagaca agctcgtagt cttcactgga gtcagcggat cggggaagtc gtctctcgcg
     6661 ttcgacacgc tctacgccga ggggcagcgg cgctacgtcg agagcctatc ggcctatgcc
     6721 cggcagttcc tcgaccagat ggagaagccc aacgtcgagc tgatcgaggg gctctcgcca
     6781 gccatcgcca tcgagcagaa gacggcgagc cggaatcctc gctcgaccgt ggccaccgtg
     6841 accgagatcc acgactacat gcgggtgctg tgggcgcggt gtggtgtcca gcattgccat
     6901 cagtgcggca agcccatcag ttcgcagacc cccgaccaga tggtggatcg cctgatggcc
     6961 ctcgaggagg gcactcggct ccagattctg gccccggtgg tcctggaccg aaaaggggag
     7021 tacgcggacg ttttcgagga ggcccgccgg gacggatacg cgcgggcccg cgtcgatggg
     7081 caggtgatcg acctctccga ggagatccac ctgtccaagc gccgcaagca ccgcatcgag
     7141 atcgtggtgg accgtctcgt gattcgcccg cagagccagg cgcggcttgc cgagtccatg
     7201 gaaaccgcgc tgaagttggg cgacggcatg gtgctcgtcg acgtcgtgga ttctgaggag
     7261 ttgttgttta gccagcatgc cgcctgcccg gactgcggga tcagcttcgg tccgctggaa
     7321 cctcagatgt tctcattcaa tgccccccaa ggcatgtgtc ccgagtgcag cggcatgggg
     7381 accaccctcg agatggatga gacccttgtg gtgaccgatc cggatcgctc gctgagcgag
     7441 ggggccatcg aactactggg caatccggag acccagcata cgaagcatgt tcttgaagcc
     7501 cttcaccgcc attttggtgt cgatatcgat cgtccgtttc gggagcttcc tctggaacac
     7561 aggaggatcg ttctccaggg ctcaccggac acggtccgct tccggtaccg cacccgcaac
     7621 gggcacacgt gggagtactc gcggacctat gacggtgtcc tctcgcaggc ccagcgacgg
     7681 ttcatcaaca gccagaccga cgaggagcgg gaggagctgg ggcagttcct ctccaacacg
     7741 ccctgcccgg catgcaaggg ggggcggctg aagccccaaa gcgccgcggt
//
LOCUS       DAOSPL010000019         7682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_9519_length_7682_cov_5.882654, whole genome shotgun sequence.
ACCESSION   DAOSPL010000019 DAOSPL010000000
VERSION     DAOSPL010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7682
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9519_length_7682_cov_5.882654"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1743)
                     /locus_tag="PLQ54_00680"
     CDS             complement(40..1743)
                     /locus_tag="PLQ54_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3854 domain-containing protein"
                     /protein_id="HOS91802.1"
                     /translation="MAVSLWDNWRRVTRREPCPVCGKGDWCLVARDGSQARCMRRESP
                     HSVPAGGWLHPLVPGRIAPRPDAMVSPVGSPRRVAPLEILDQVYEALLGQLSLSDHHA
                     AQLRARGMDDAEIAAGGYRTFTRHRRADIARRLADQFTPGALTRVPGFYIDDNGHSAY
                     LTLAGPAGLLIPCRDAHGHLTGLQIRRDQGSPRYLPLTSYTENKPRRGGTPVTVSLLR
                     HVAYPPQVTDPRVFITEGTIKAQMAAWRLGAIVISSPGVACWNAAGIVETAVKLAKPG
                     GSVVVAYDADKMRKSAVAQAEASLCRALEAAGLSVRVARWDAETGKGLDDLLVAGGTF
                     RLEEPEDFETSSRLPSRVIEVHLPPPQPSSGLFESRAYTADEVRQHIYTTAAQLLEEG
                     VPGLHLFTSPPGSGKTTCILRALADLSRAGRLVDWRDRTPVQRRVLFLTDTKEALAEL
                     VSAVPGLREIAAFREGRSDDPASPWYCARKAEVDELAHARHNAARELCWTCYERLPHC
                     PYMRSKQQAERQNILLAPKAVALSDPSFLDGPADLSPFSAVVVDESLTPALLEDVRHP
                     A"
     gene            complement(1945..2994)
                     /locus_tag="PLQ54_00685"
     CDS             complement(1945..2994)
                     /locus_tag="PLQ54_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOS91803.1"
                     /translation="MSRKRVSTAGLIADLFRAHPAVAGDGDGRLSSLSPVPSPVEATA
                     RTRDGQLLEIPLDQIHVGPHQARQGFDEERLQELAESIRAHGVIEPVLVTPHPEGSGY
                     LLIAGERRVRAARQAQRTTIPATVRDALSEADILVLSLTENLQREDLSPTERAEGIAA
                     LQKATGESLSAVAARLGYTRARVCQLMGYRRLPPAIYEALTQKRITEKQAQAILRVDP
                     VSPAAQRALFEACVEHHLHDAEAGRLARRLMEQPTASPAAVVSGFLEQRRPAESRRVT
                     ASHRRLASLTMRLQRQEVVLADLAAQVHHLAKTVHAEERPTAQELQSAHQVVEALESH
                     LKQIEGGLRALRRRG"
     gene            complement(2976..>3596)
                     /locus_tag="PLQ54_00690"
     CDS             complement(2976..>3596)
                     /locus_tag="PLQ54_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOS91804.1"
                     /translation="PASSAHRLLTEPEADLSALPQETDIPGVDLVASHPVMSTLDAQL
                     STVVDREFRLRTGLASLTAGARGYGWVVLDSGPYMGILSINVLTAAHAVVLPLPPEFV
                     VLLGGRMLLDYVQQIRDHFNPELRILGILPTMVDARTRHAREMLDQLRSVFGDLVFEQ
                     AIPYTVRLKEAPILGASILSYDPSCRAAEAYRALAGEVQARCRASV"
     assembly_gap    3597..3606
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3607..3788)
                     /locus_tag="PLQ54_00695"
     CDS             complement(<3607..3788)
                     /locus_tag="PLQ54_00695"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS91805.1"
                     /translation="MTVLAIANAKGGVGKTTTALTLAAALADGPASKKGSPRVLLVDL
                     DPGGGLTGILRRPVEDP"
     gene            complement(3785..4000)
                     /locus_tag="PLQ54_00700"
     CDS             complement(3785..4000)
                     /locus_tag="PLQ54_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOS91806.1"
                     /translation="MGADSVENEVLTADEVAHLLRLSPKTVRMKAAVGELPAKKVGKV
                     WRFVRSDIEAYLRHSEADHVAVGRGLR"
     gene            complement(4318..5304)
                     /locus_tag="PLQ54_00705"
     CDS             complement(4318..5304)
                     /locus_tag="PLQ54_00705"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS91807.1"
                     /translation="METVPSPHRRRFLSTTMTAGLSAWATRMALAAPDDIMPADHLPR
                     RPYGSTGRLLSIIGLGGIVVSQIEQSEANDIVSRAFDRGVNYYDVAPTYSNAQERLGP
                     ALEPYRDRVFLACKTAERHAAEARAELEQSLKLLRTDHLDLYQLHALSTVEDVQTVFG
                     PGGAFEVFLQARDKGQVRYLGFSAHTEEAALLALDQFDFDSVLFPFNAVCYHQGHFGR
                     KVLERARDRGAARLALKALAWTPWPEGAERTHPKCWYQPITDRRLAFLALRFTLDLPL
                     TAAVPPGDPRIFEMAVDLGLRYRPLEREERQMLWASMDGVAPIFRRPGSDAA"
     gene            complement(5429..6127)
                     /locus_tag="PLQ54_00710"
     CDS             complement(5429..6127)
                     /locus_tag="PLQ54_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS91808.1"
                     /translation="MARKLSIIIPVFNEARYIDQVLERLRELPIAREIIVVDDGSTDG
                     TRERLRELRQQWPPEDLVLHFLAENRGKGYALREGIALASGDVVAIQDADFEYDPSEL
                     PELAAPIFERKCRVVYGSRFRGTIQGMAPLNRAFNRIISAFASILYGQRITDEATCYK
                     LFEAAFLKSIPLRCERFEFCPEVTAKVRRRGERILELPIRYVGRTTLEGKKIRWWDGV
                     VALWTLLRYRFWRG"
     gene            complement(6294..7112)
                     /locus_tag="PLQ54_00715"
     CDS             complement(6294..7112)
                     /locus_tag="PLQ54_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HOS91809.1"
                     /translation="MGLLNALTFDIEEHFQVSGFADHIAPERWDQYESRVVPNTERLL
                     GLLSQMGLRATFFFLGWVAERHPDLVRAAAEAGHEIACHGYAHRLVYDLTPEQFRDDL
                     RRSLDAICPHAGGPILGYRATSFSITERSLWALDILKDEGLVYDASVFPVKRARYGIP
                     DAPRAPYTHPNGLIEFPSTVGRLGKLKIPVGGGYFRLFPYALTRHAIRQVNAEGRPAI
                     AYFHPWEFDPKQPRMLGDRVNSFRHYVGLKHTEAKLRRLCADVSFAPARVVLGI"
     gene            7415..>7682
                     /locus_tag="PLQ54_00720"
     CDS             7415..>7682
                     /locus_tag="PLQ54_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91810.1"
                     /translation="MKKSVVTLIVAVCVLGMSSTAFSYQFTSAGGWYVDWGLEIVLDN
                     GWGADPGKHLDWNKSSDGGTTWTALTGGADLLYNNGTETVCYTLE"
BASE COUNT         1396 a         2527 c         2538 g         1211 t
ORIGIN      
        1 acccggaggc gcccttccat tcctctgatc tgcacgttct catgcgggat ggcgcacatc
       61 ctccaacaga gccggagtca ggctctcatc gaccaccacg gcggagaagg gcgatagatc
      121 ggcgggtcca tcgaggaagc tcggatcgct gagggccacg gccttggggg ccagcagaat
      181 gttctggcgc tcggcctgtt gcttggaacg catgtagggg caatggggta accgttcgta
      241 gcaggtccag cacagctccc gcgccgcgtt gtgccgggcg tgggcgagtt cgtccacctc
      301 cgccttccgc gcgcagtacc acggactggc cggatcatcg gatcggccct cgcgaaacgc
      361 ggcgatctcc cgcaaaccgg gcacggcgga caccaactcg gccagggcct ccttggtgtc
      421 ggtcaggaac agcacccgcc gctggacggg agtccggtcg cgccagtcca cgagacggcc
      481 cgcccgcgag agatcagcca gcgctcgcag aatacatgtg gtcttcccgc tgcccggggg
      541 cgaggtgaac agatgcagac cgggcacgcc ctcctcaagc agctgcgccg cggtggtgta
      601 gatatgctgg cgcacttcgt ccgccgtgta ggcccggctc tcaaacagac ccgaagacgg
      661 ttggggggga ggcagatgga cctcaatgac cctcgaaggc agccgcgagg aggtctcgaa
      721 gtcctcgggc tcctccaaac ggaaggtccc tccggccacc agcaggtcat ccagcccctt
      781 gccggtctcg gcatcccatc gcgccacgcg cacactcagc cccgcagcct ccagggcccg
      841 gcacaacgag gcctcggctt gggccaccgc ggactttctc atcttgtccg cgtcgtaggc
      901 gacgaccacc gatccacccg gtttagctaa cttgaccgcc gtctccacaa tgcccgcagc
      961 gttccagcac gccacgccgg gcgaggagat gacgatcgcc cccaggcgcc aggcggccat
     1021 ttgcgccttg atggtgccct cggtgatgaa cacccgtggg tccgtgacct gaggcggata
     1081 ggccacgtgc cgcaaaagcg acacggtcac cggtgtgccg cctcgccgag gcttgttctc
     1141 cgtgtagctg gtcagcggca gatagcgggg gctgccctga tcccgccgga tctgtaggcc
     1201 cgtcagatgg ccgtgggcgt cgcggcaggg gatcaagagc ccggcggggc cggcgagggt
     1261 gagataggcc gagtggccgt tgtcgtcgat gtagaacccg ggcacccgcg tcagggcacc
     1321 gggagtgaac tggtcggcca agcggcgggc gatatcggcc cggcggtgac gggtgaaggt
     1381 ccggtatccg ccggctgcga tctccgcatc gtccatgccg cgggctcgga gctgagccgc
     1441 atgatgatcg gaaagtgaga gttgtcccag cagggcctca tagacctggt cgaggatctc
     1501 gagtggcgcc acccggcggg gagaccccac cgggctcacc atggcgtctg gccggggcgc
     1561 gatccgaccg ggcaccaagg ggtggagcca gccgcccgcg gggacggagt ggggggactc
     1621 gcggcgcatg caccgggcct gggacccgtc ccgggccacc aggcaccagt cgcccttgcc
     1681 acacacaggg caaggctccc gccgagtcac tcggcgccag ttgtcccaca acgaaacggc
     1741 caactccggc tccccaaagc ctccacaaag aggcggatgc cccagttctc cagggccacc
     1801 gaggccggat ttgaccgaga acgccggacc acatacacta aaggtccata gcttgtccag
     1861 gacagttctc tgtctttggg aacctcgccg ctcctgtaag cggcgaggtt ctcggccttc
     1921 tagggcccgt cgatctccct cgatctaccc acgacgacga agggcgcgca gccccccctc
     1981 gatctgcttg agatgactct ccagcgcctc gacgacctgg tgtgccgatt ggagctcctg
     2041 tgccgtcggg cgttcctccg cgtgaaccgt cttcgccagg tggtgcacct gggcggccaa
     2101 atcggccaac acaacttcct gccgctgcag gcgcatcgtc aaactagcta aacgccggtg
     2161 actcgccgta acccggcgcg actccgcggg gcggcgctgt tccaggaatc ccgacaccac
     2221 ggccgcgggg gacgccgtcg gctgctccat caagcgccgg gccaaccgcc ccgcctcggc
     2281 gtcgtgcaga tgatgttcga cgcaggcttc aaacagggcc cgctgcgccg ccgggctcac
     2341 cggatccacg cgcaaaatgg cctgcgcctg cttctccgta atacgctttt gggtcagtgc
     2401 ctcatagatg gccgggggca ggcgccgata gcccatgagc tggcagaccc gagccctggt
     2461 gtaacccagg cgcgccgcca cggccgacag gctttccccc gtggccttct ggagagccgc
     2521 aatcccctcg gcccgctccg tgggcgaaag gtcctcgcgc tgcagattct ccgtgagaga
     2581 cagcaccaga atgtcggctt ccgataaggc gtcgcgcacc gtggccggga tggtggttcg
     2641 ctgggcctgc cgggccgctc gcacgcgccg ctcacccgcg atcagcaggt accccgaacc
     2701 ttcggggtgc ggcgtgacca gaacgggctc gatgacccca tgggcccgga tggactcggc
     2761 cagctcctgc aaccgttcct cgtcgaatcc ctggcgcgct tgatgaggcc ccacgtggat
     2821 ctggtccagg gggatctcga gcagttggcc gtctcgggtg cgagccgtcg cctcaaccgg
     2881 cgaggggacc ggggaaaggc tcgacagccg gccatcgccg tctcccgcca cggccgggtg
     2941 agcccggaac agatcggcga tgaggccggc cgtgctcaca cgcttgcgcg acaccgagcc
     3001 tgcacctccc ccgccagagc tcgataagct tccgcggccc ggcacgacgg gtcgtagctg
     3061 agaatggacg ctccgagaat cggagcctcc ttgaggcgca ccgtatacgg gatggcttgc
     3121 tcgaacacca gatcgccaaa gacggatcgg agctgatcca gcatctccct ggcatgccgc
     3181 gttcgcgcat ccaccatggt gggcaggatg cccaggatcc ggagctccgg gttgaagtgg
     3241 tctcggatct gctggacgta gtccaggagc atccggccgc cgagcagcac gacaaactca
     3301 gggggcaggg gaagcacgac cgcatgagct gcggtgagca cgttgatgga gaggatgccc
     3361 atataggggc ccgagtccag cacgacccag ccgtagccac gggcgccggc cgttaagctg
     3421 gctaaaccgg tccgcaaacg gaactcccga tccaccaccg tagagagctg ggcatccagg
     3481 gtagacatga caggatgaga ggccaccaga tccacgccgg ggatgtcagt ctcctggggc
     3541 agggcactca gatccgcctc gggctccgtc aagagccgat gggccgaact cgccggnnnn
     3601 nnnnnngggt cctccacggg acggcgcaga atgcccgtga ggcccccacc gggatccagg
     3661 tcgaccagga ggactcgggg cgagcccttc ttgctggccg ggccatccgc cagggccgcg
     3721 gccaaagtca gagccgtggt cgtcttgccc acgcccccct tggcattggc gatggccagg
     3781 acggtcaccg caagcccctc cccacagcca cgtgatcggc ctcggaatgg cggaggtagg
     3841 cctcgatatc actccggacg aagcgccaga ctttcccgac cttcttggcc gggagctccc
     3901 ccacggccgc cttcatgcgc accgtcttgg gcgacagccg cagcaggtgg gcgacttcgt
     3961 cggcggtgag cacctcgttc tccacggagt ccgctcccat cccgcgatcc ccttgcccgt
     4021 tcgcaaacag gcgcaaaggc acacactggg gcgctggttc ccctaaaggg acacaacggg
     4081 atattcaggc gctcctcgcc gactcctgtc ggctctttgc cctcataccc aggaaataga
     4141 ggagggtagg ggagggcaga agggcactgg cggtcactcg gggctccagc ggcccatggg
     4201 cctggttgcg gaggggcgcc ctgggggaaa ccacgcggtc agggcgtggt gggccccaac
     4261 gcgcccattc caggaccccg aacgcggcac gaagccgcgc gtgcggtacc tgggcggcta
     4321 ggccgcatcg cttccgggcc ggcggaaaat gggcgcgacc ccgtccatgg aggcccagag
     4381 catctggcgt tcctcgcgct ccaggggccg gtaccgcagc cccagatcga ccgccatctc
     4441 gaaaatgcgc ggatctcccg gaggcacggc cgcggtgagc ggcaggtcca gggtgaagcg
     4501 cagagccaga aaagccaacc ggcgatcggt aatgggctga taccagcact tgggatgggt
     4561 acgctcggcc ccttcgggcc agggggtcca agccagggcc ttgagcgcca gtcgagcggc
     4621 accacgatcg cgagcgcgct ccaggacctt tcggccaaag tgcccctggt gatagcacac
     4681 cgcgttgaac gggaagagca cgctgtcgaa atcgaactgg tccagcgcca gcagagccgc
     4741 ttcctcggtg tgggccgaga atccaagata gcgcacctgg cccttgtcgc gcgcctggag
     4801 gaacacttcg aaagccccgc cgggcccaaa gacggtctgc acatcctcga ccgttgagag
     4861 cgcatggagt tgatagagat cgaggtggtc agtccggagg agcttcaggg attgctcgag
     4921 ctcagcccgg gcctcggcgg catggcgctc cgccgtcttg caggccagga agacgcgatc
     4981 gcgatagggt tccagggccg gccccaggcg ttcttgggcg ttgctgtagg tgggggccac
     5041 atcgtaatag ttgaccccac gatcgaaggc ccgggaaacg atgtcgttgg cctcagactg
     5101 ctcgatctgg ctcaccacga ttccaccgag gccaatgatg gagagcagtc gccccgtgga
     5161 accatagggg cgccggggaa gatggtcggc cggcatgatg tcatccggtg ctgccagtgc
     5221 catccgcgtg gcccatgcgc tcagtccagc ggtcatggtg gtgcttagga accggcggcg
     5281 atggggggat ggaacggtct ccacgggctt ctcctccaag gtttggctga ttgttccagc
     5341 ggttccggcg cgggagtggt tcccccatgc tgcggttccc gagccatact gcggccaccc
     5401 ccacgagccg cgcgcggcgc ggatactcct aaccacgcca gaagcggtag cggagcaagg
     5461 tccacaaggc gacgacgccg tcccaccagc ggatcttctt gccctccagg gtggtacggc
     5521 cgacatagcg gatggggagc tcgaggatcc tctctccgcg ccggcgcacc ttggcggtga
     5581 cctcgggaca gaactcgaac cgctcgcacc gcaacgggat ggacttgaga aaggccgcct
     5641 cgaacagctt gtagcaagtc gcctcatcgg tgatccgctg gccgtacaga atcgaagcaa
     5701 aggcgctgat gatccgattg aacgcgcggt tgaggggagc catgccctgg atggtccccc
     5761 ggaagcgtga cccgtacacc actcggcatt tccgctcgaa gatcggggcc gccagttcgg
     5821 ggagctcgga ggggtcatac tcgaaatcgg cgtcctggat ggccaccaca tcacccgacg
     5881 ccaacgcgat gccctcgcgc aatgcatagc ccttcccgcg gttctcggcc aggaaatgga
     5941 ggaccaagtc ctccgggggc cactgctggc ggagttcccg cagccgctcg cgcgtgccgt
     6001 cggtggaacc gtcatccacc acaatgatct cgcgcgcgat gggcaactcg cgcaaccgct
     6061 ccaggacctg gtcgatatac cgggcctcat tgaagaccgg gatgataatg gagagcttcc
     6121 gggccatgaa cctgccttac cccagaggat ggctggggaa ccacggactc cgccagtgcc
     6181 ggagcgggtg ggatggtgcc gcgacattgt aatccagggg ccgagttcgc atgtgggaga
     6241 gggaccctat ccggccccag gccactgccg ggcacaccgt ctgaagacgg gttctagatc
     6301 cccaacacga cccgggccgg cgcaaaggac acatcggcac acaagcgacg cagcttcgct
     6361 tccgtgtgct tcagccccac ataatggcgg aagctgttca cgcgatcccc cagcatgcgg
     6421 ggctgctttg ggtcgaactc ccacggatgg aaataggcaa tggccggccg accttcggca
     6481 ttgacttgcc ggatggcgtg acgagtcaac gcataaggga acagccggaa gtacccgccc
     6541 cccaccggga tcttcagctt ccccaatcgg cccacggtcg acgggaactc tatcagtcca
     6601 ttggggtgcg tatagggggc ccgaggcgca tccgggattc cgtaccgcgc gcgcttcacg
     6661 gggaacacgc tggcatcgta gaccaggccc tcgtccttga gaatgtcgag agcccagagg
     6721 cttcgctcgg tgatggagaa ggaggtggcg cggtagccga ggatgggacc tcccgcatgg
     6781 gggcatatgg cgtcgagcga ccggcggagg tcatcacgaa actgctcggg agtcaggtca
     6841 tagactagcc ggtgggcata tccgtggcag gcgatctcgt gtcccgcctc cgcggcggcc
     6901 cggacgagat ccggatgccg ttccgccacc cagcccagga agaagaaggt ggcccggagt
     6961 cccatttggc tcaggaggcc gagcaaccgc tcagtgttgg gcaccacgcg gctctcatac
     7021 tgatcccaac gctccggtgc gatgtggtcg gcgaagcccg agacctggaa gtgctcctcg
     7081 atgtcgaagg tgagcgcatt gagcaacccc aagacctgcc tctcgccctt tcagcgtacc
     7141 tcataccgtc ggcacgggct ccatcacaga gctcaaagaa cggccggcgg gctccgaggt
     7201 tctcgaccgt taacgagtgc ttcgcggatt cctctcggga accgggatac atttgcagcg
     7261 cctgggtgtt ccggcctaat gcttgacggg cgtgtaacac tacgctaaac tggagcagtc
     7321 aatgatcact ggacgtcacc tggccttggg agcggcacgg tacctgtgac acggggttcc
     7381 gcaggggtga cagcaccaag agggagcgta gcagatgaag aagtccgtgg tgacactgat
     7441 tgtggcggtg tgtgtcctgg gaatgtcctc gacggcattc tcataccagt tcacctcagc
     7501 gggtggctgg tacgttgact gggggctgga aatcgttctg gacaacggct ggggagccga
     7561 tcccggcaaa cacctcgact ggaacaagtc gagcgatggt ggaaccactt ggactgccct
     7621 gaccgggggc gcggacctgc tctacaacaa tggcacggag actgtgtgct acacgctgga
     7681 gg
//
LOCUS       DAOSPL010000020         7647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_9578_length_7647_cov_2.712724, whole genome shotgun sequence.
ACCESSION   DAOSPL010000020 DAOSPL010000000
VERSION     DAOSPL010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7647
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9578_length_7647_cov_2.712724"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PLQ54_00725"
     CDS             <1..477
                     /locus_tag="PLQ54_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91811.1"
                     /translation="THVNAAIQVVIDRWAEQLGPVMSRPTSAFELGRDLVSLRASLAR
                     VFVLAGHPSLPEMARVTLLGAVDAMAADAQRSLEDGIRRQLTGSGSGKGTLDAMLGVL
                     RANPFTDASRHRLDDTGRMSMPAIPEAIPTQQAPRRSAPDEARPSRFAHRIISPPN"
     gene            524..2077
                     /locus_tag="PLQ54_00730"
     CDS             524..2077
                     /locus_tag="PLQ54_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005521376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS91812.1"
                     /translation="MPDFTETKEILTRYLRARVPVVVIRSMEPVRVMELLTQSAAEMG
                     AMAYTVYSRTEGLRDLMSNQVVSDDTSLSAALEMSRQTFKTRSNVNVVFTEIDDLEQE
                     SPTSRHFAELARLAEQRQGTLIFLTDRPVWSGLSRLGMSVSLDLPTIDELTEVLEGFI
                     EANRPTTDIEWGFDEVRQAAEILRGITETEAINVIATMLARRRLLNSEIPELSQFKDR
                     IFGDLSGLERIPLKEDYAIGGLRTLRDWLDKRERAMKVDLSQTSFHPPKGVLLVGVPG
                     CGKSLSAKAVAQAWQLPLYRLDMASVLGMYVGQSESQLREALETADRVAPCVLWIDEI
                     EKGLANGTGDSGVSRRLLGQFLFWLQESTSKVFLVATANDVTSLPPELLRKGRFDEMF
                     FVDLPDRQDREEIIRMYFRKHLSHDIPPDLTEQVVTRTEGFSGSDLAAVIHELGLLRH
                     TEHRSEVPNDGEVLRAFSDVVPFSATNPEELAAIRQWAQGRCRPAGQISDGNGPVAQP
                     GAVRRVFLQ"
     gene            complement(2135..2881)
                     /locus_tag="PLQ54_00735"
     CDS             complement(2135..2881)
                     /locus_tag="PLQ54_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91813.1"
                     /translation="MSSELTQVANAIRSVGASAGPVTTGVASVRQRASRLAGGIPRGA
                     PSAGAAIGALERAVAQLNAAAEQLATFSQRAEGFAQRLVMGDSPQSLALARAREDAEN
                     AFVSTGDSPAPAGSATSETPAWAVERGLEGFDVGLPEYSDNPIREDWGEHGLTKDDYL
                     WAATSWREVVRPGLERGLSQDDFAAMDAQSDAPPLRRLADICGMYLGDPITLSEGPGG
                     RWTVTSGRHRLEAARLIGDTRLFGKLFRRQ"
     gene            complement(2878..3171)
                     /locus_tag="PLQ54_00740"
     CDS             complement(2878..3171)
                     /locus_tag="PLQ54_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91814.1"
                     /translation="MTSQAYEAAARDAAQLSAYTAQLGRGLADLSKRTTATIGGTATG
                     EDRAMTGQLSDISRRLASASRAFEDAAQAARRAAREAAEAEAQQARQAQQGRR"
     gene            complement(3168..3593)
                     /locus_tag="PLQ54_00745"
     CDS             complement(3168..3593)
                     /locus_tag="PLQ54_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91815.1"
                     /translation="MALPEPTPTEDPRDVAYAALEAQVAADAALTPVRDQWVAVIDSK
                     YEGLVDPVIQAEPFTVEQIKAAHDEYRSNPDWGSQVRLLHNGDWGERKERRFMWVTIV
                     DLDMSSKDDVTSWCRANRPGPADDPDHFCVGFKMKTPYG"
     gene            complement(4033..4293)
                     /locus_tag="PLQ54_00750"
     CDS             complement(4033..4293)
                     /locus_tag="PLQ54_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91816.1"
                     /translation="MSQLQQIKQSLTALGQQARQTGAGLAQHSQQFRQQGQQVAQLIG
                     GSAQSKDKQMMAALDQAAKAVQQAQRALDECAKAAQSYASSI"
     gene            4690..5148
                     /locus_tag="PLQ54_00755"
     CDS             4690..5148
                     /locus_tag="PLQ54_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91817.1"
                     /translation="MIAAGVVGSVLLVVAGIQLGARGESSENATQPAGSALDTSEVTQ
                     AQPDIATPEGTVPPEQPAVTSTPVLRVPADGTWIVVLESKPKTESDVSGALTLATTIG
                     SSGPSVEVIDSDVTPGLNPGYWVVIHGEFATRAEAHGLLRRDGRWLRFAD"
     gene            5138..6202
                     /gene="mqnC"
                     /locus_tag="PLQ54_00760"
     CDS             5138..6202
                     /gene="mqnC"
                     /locus_tag="PLQ54_00760"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HOS91818.1"
                     /translation="MPIDGVLQRAVGGGRLERDELLELYEHANLLSLGDAADRVRQRM
                     HPDRVVTYVVDRNINYTNVCVSRCRFCAFYRDEGHPEAYVLGFDTIAEKIRALCDAGG
                     TQVLMQGGLHPSLTIEYFEHLLRFIKQRFAVQLHSLSPPEIVHIAGVSGLNTADTLRR
                     LRDAGLDSLPGGGAEVLSDRVRQAVSPAKCSAGEWHTVMEQAHALGMPTTATMMFGHG
                     DSPADRVDHLLAVRALQDRTGGFTAFIPWTFQPANTAMAHRPTLGGHEYLRTLAISRL
                     ALDNVRNIQASWVTQGAKVAQLALRFGANDMGSTMMEENVVRAAGVSFRLDRRGISSL
                     ITAAGFAARQRDTRYRRIGG"
     gene            6205..7080
                     /locus_tag="PLQ54_00765"
     CDS             6205..7080
                     /locus_tag="PLQ54_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HOS91819.1"
                     /translation="MNARVLAVIGGSGAYDLLRDQSLEGPCAGPVETPYGNSAPVRRI
                     DCGEGAAVMFMSRHGERGYEVAAPFVNYRANIYALKSLGVSHIVAWSGPGAIDPSLEV
                     GQYVVPDDLLDATRGRASTFFEGKGHGFIRQNPVFCPVLRSLLLSACASTSRPVRDGA
                     TYVCTQGPRLETPAEIRQYAILGAHLVGMTLIPESFLARELAMGYAPLCYITNYAEGV
                     RERPYESGTLFEGLATTDERQRVRDAVSALPDLLRSVARAWREIAPDCACHTSMERFV
                     RSGRLGPDWRTWTDR"
     gene            complement(7086..>7647)
                     /locus_tag="PLQ54_00770"
     CDS             complement(7086..>7647)
                     /locus_tag="PLQ54_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91820.1"
                     /translation="ESLRIEIAKRFGREWEISESTRNSQTVFTIASSRSRASVDDYPG
                     VALDLQSTSSEQSFTYTETLDLARFLPVPSVRKDFANTEIAFRVKMPGKITSYSPTFR
                     GTQESDDTVLFTWKVSDLDRDEPRSVRVTSSAKRLDRGFLIALETIVFGGAVLSILTS
                     SRAVRFFRRVFLDDDAAAEDIIGEPG"
BASE COUNT         1245 a         2650 c         2487 g         1265 t
ORIGIN      
        1 acccacgtga acgccgccat ccaagtagtc atcgaccggt gggcagagca gctcgggcca
       61 gtcatgagcc gaccgacgtc ggccttcgag ctcggccgcg atctggtgtc gctgcgggcc
      121 tcgctggcgc gtgtgttcgt cctcgcaggg cacccatcgc taccggagat ggcccgcgtc
      181 actctgctcg gcgcggtgga cgcgatggcg gctgatgcgc agcgaagcct ggaggacggc
      241 atccggcggc agctcaccgg aagcgggagc ggcaagggca ccctggacgc catgctcggg
      301 gtccttcgtg ccaacccatt caccgacgca tccagacacc ggctcgacga caccggccgg
      361 atgagcatgc cggccatccc agaagccatt cccacgcaac aggcaccccg acggtcggcg
      421 cccgacgagg cacggcccag ccggtttgct caccgaatca tctcaccacc gaactgagct
      481 tcagtacgcc tggctccctg cagaactcaa cgaaaggact gtcgtgcccg acttcaccga
      541 gaccaaggag atcctgacgc ggtacctgcg ggcgcgggtt cccgttgtcg tgatccggtc
      601 gatggagcca gtgcgcgtca tggagttgct gacccagtcc gcggcggaga tgggcgcgat
      661 ggcctacacg gtgtacagcc gcacggaggg cctgagggac ctcatgtcca accaggtggt
      721 ctcggacgac acttccctgt ccgcggccct ggagatgtct cgccagacgt tcaagacgcg
      781 cagcaacgtc aacgtcgtgt tcaccgagat tgacgacctc gaacaggagt cgccgacgtc
      841 gcgtcacttc gccgagctcg cgcgcctcgc cgagcaacgc caggggacgt tgatcttcct
      901 caccgaccgt cccgtctggt cggggctctc ccgcctcggc atgagcgtct cgttggatct
      961 cccgacaatc gacgagctga ccgaagtact cgagggcttc atcgaggcca accgtccgac
     1021 cacggacatc gagtggggtt tcgacgaggt gcgccaagcc gcggagatcc tccgcggcat
     1081 caccgagacc gaggccatca acgtcatcgc cacgatgttg gctcgacgcc ggctcctcaa
     1141 ctcggagatc ccggaactct cgcagttcaa ggaccgcatc tttggtgact tgagcgggct
     1201 ggagcgaatc ccactcaagg aggactacgc gatcggcggc cttcggaccc tgcgcgactg
     1261 gctggataag cgagagcgtg ccatgaaggt cgacctgtcg cagaccagct tccacccacc
     1321 caaaggggtc ctcctcgtcg gtgtgccggg gtgcgggaag tcactttcgg ccaaggcggt
     1381 tgcccaggca tggcagctgc ccctgtatcg cctcgacatg gcctcagtgc tcgggatgta
     1441 cgtcggccag tccgagtccc agctccgtga agcgctggag acagccgacc gggtcgctcc
     1501 ttgtgtgctc tggatcgacg agatcgagaa gggcctggcc aacggcaccg gagactcggg
     1561 tgtgagccga cgactcctcg ggcagttctt gttctggctg caggagtcga cctcgaaggt
     1621 gttcctcgtc gcgaccgcga acgacgtgac gtcgctgcct cccgagctac ttcgcaaggg
     1681 ccgcttcgac gagatgttct ttgtcgacct tcccgaccgc caggatcgtg aggagatcat
     1741 ccggatgtac ttccggaagc acctgtcgca cgacatcccg cccgacctca ccgaacaggt
     1801 cgtcacccgc accgaaggct tctccgggag cgacctcgct gctgtcatcc acgagctggg
     1861 gctgctcagg cacaccgagc accgctctga ggtcccgaac gacggcgagg tgctccgtgc
     1921 cttctccgac gtcgtgccat tcagcgccac caaccctgag gagctggccg ccatccggca
     1981 gtgggctcag ggtcggtgcc gtccggccgg gcagatcagt gacggcaacg gcccggtcgc
     2041 acaaccaggc gcagtccgca gggtcttcct ccagtaaggc acggcacccc tctcagtgcc
     2101 cttggcctcg ccaatgcacc acccgtggtg gggttcactg ccggcgaaac agcttcccga
     2161 acagtctggt gtcaccgatc aggcgtgcgg cctccaaccg gtgccgcccg ctggtcaccg
     2221 tccatcgacc accgggccct tcggagagcg tgatcgggtc cccgaggtac atgccacaga
     2281 tgtccgcgag ccgacgaagc ggtggggcat cggactgcgc atccatcgcc gcaaagtcgt
     2341 cttgggagag tcctcgctca aggccggggc gaaccacctc gcgccaagag gtcgctgccc
     2401 acaggtagtc atccttggtc agaccgtgct ccccccagtc ctcacgaatc ggattgtccg
     2461 agtattccgg cagtccaacg tcgaacccct caagaccgcg ctcgactgcc cacgccggtg
     2521 tctcgctcgt ggcggacccg gcaggtgctg gactatcgcc ggtgctgacg aacgcgttct
     2581 cggcatcctc gcgggcccga gccagggcca gcgactgcgg gctgtcgccc atgacgaggc
     2641 gctgcgcgaa gccctccgcc cgctggctga acgtcgccag ctgctcagcg gcagcattga
     2701 gctgggcgac cgcccgctcc agggctccga tcgccgcacc tgctgagggt gctccccgcg
     2761 ggattccacc cgcgagcctg gaggctcgct ggcggaccga cgcgactccg gttgtcaccg
     2821 gtcctgccga ggcgcccacc gatcggatgg cgttggcgac ctgagtgagt tcagaactca
     2881 ccgtcgcccc tgctgcgctt gacgcgcctg ctgcgcctcg gcctccgctg cttctcgagc
     2941 cgcccgacgg gccgcctgcg cggcatcctc gaaggccctg gacgccgacg cgagccggcg
     3001 tgagatgtcg gagagttggc cggtcatggc gcgatcctca ccagtcgccg tcccaccgat
     3061 ggttgcggtg gtgcgcttgg acaggtctgc caggccgcgt ccgagctgtg cggtgtacgc
     3121 ggacagttgc gctgcgtcgc gtgcagcggc ctcgtacgcc tgcgaggtca tccgtacggt
     3181 gtcttcatct tgaacccgac gcagaagtgg tcagggtcgt cggccggccc gggccgattg
     3241 gcccgacacc acgacgtgac atcgtccttg gacgacatgt cgaggtcgac gatcgtgacc
     3301 cacatgaacc gtcgttcctt gcgctcaccc cagtcaccgt tgtgcagaag ccgcacctga
     3361 ctgccccaat ccgggttact gcggtactca tcgtgcgccg ccttgatctg ttcgaccgtg
     3421 aacggctccg cctggatcac ggggtcaacg agtccctcgt acttgctgtc gatgacggcg
     3481 acccactggt ctcgaaccgg ggtgagagca gcgtctgcgg cgacctgtgc ctccagtgcc
     3541 gcatacgcaa cgtcccgcgg gtcctcagtg ggcgtgggtt cagggagcgc cacctcttca
     3601 acaggttcct ccacaggcgt ctccacctgc gggggagccg ccgccggcgc caccggctcg
     3661 ggctcgggtt cgccgcgccc ggatgcgaac aggccccaga gagcaactgc ggcgacacct
     3721 gccgcggcga tcttgaccca cagcggcagg cctgtcggct gggacggcgc gacacgagga
     3781 accccatcgg gagtcgcggt cgatgccgag cgggccgccg atccataccc atcctgcgct
     3841 gagcccgacc ccaggaactg attcgacgtg gagtggctgg ttggcggcac ctggcgagtt
     3901 ggtgagggag gggccgtcgg cgatgcaacc acggtcgcct ggggccccac cggggtgcca
     3961 cagacgtcac agcgcgcgac cgaagcagcc agcggggaac cacacaaggt gcacttcatg
     4021 aaggtggcac gctcagattg agctcgcgta ggactgggcg gccttcgcgc attcgtcaag
     4081 tgcccgctgt gcctgctgga cggccttcgc cgcctggtcc agggccgcca tcatctgctt
     4141 gtccttgctc tgggcgctcc cgccgatcaa ctgggccacc tgttggccct gttgacggaa
     4201 ctgctggctg tgctgagcca gccccgcacc ggtctgacgc gcctgttgcc cgagcgcggt
     4261 cagtgactgc ttgatctgct gcaactgcga cattccggtt ccccttcccg tggccttgtc
     4321 accccgacac taggcgagtc gcacccagaa gcgcagccaa acgctatgtt gccttgacct
     4381 ctcacaacgc gggagagttg gcgggtgacc gactccccga ccacccgatg tccgctgtgc
     4441 gggaccgcga ctggggcggc ccggtgtccc gtgtgcggga gcccggtcgc tgggcacgcg
     4501 acgcccccca ccccgcaccc gacggttcct gttgcgctgg ccagcaccca ggtccacggt
     4561 gccgcccctt cgtcaccagg acctcgcgcc ccagggttgg agaccagtgt ccgggacgct
     4621 caaccttccc tggatctgca cccgccacct ggccatgacg acgtccgtgc ggccgcgaag
     4681 cgacgcgcca tgatcgccgc gggtgtcgtg gggtccgtac tgctcgttgt tgccggaatc
     4741 caactgggcg ctcggggcga gagctcggag aacgcgactc agcccgcagg gtcggccctg
     4801 gacaccagcg aggtcacgca ggcccagcca gacatcgcca cacccgaggg gacggtgccg
     4861 cctgagcaac cggcggtgac gagcacgccg gtgctgcgcg tacccgctga cggaacgtgg
     4921 atcgtcgtgc tcgagtcgaa gcccaagacg gaaagcgatg tatccggcgc gctcacgctg
     4981 gccaccacca tcggaagctc gggcccgtcg gtcgaggtca tcgactccga cgtgactccg
     5041 ggactcaacc cggggtactg ggtggtcatc cacggcgagt tcgcgacacg ggccgaggct
     5101 cacggcttgc tgcgacggga cgggcggtgg ctccgctttg ccgattgatg gggtactcca
     5161 gcgggctgtg ggcggggggc gtcttgagcg cgatgaactg ctcgaactct acgaacacgc
     5221 caacctgctg tcgcttggcg atgcggcgga ccgcgtgcgc caacggatgc accccgaccg
     5281 cgtcgtcacc tacgtggtcg accgcaacat caactacacg aacgtgtgcg tctcgcggtg
     5341 tcgcttctgc gccttctacc gcgacgaggg gcatccggaa gcgtacgtgc tcggcttcga
     5401 caccatcgcc gagaagatcc gagcattgtg cgatgccggc ggcacccagg tgctcatgca
     5461 aggcggcctg cacccctcgc tgaccatcga gtacttcgag catctgctgc gcttcatcaa
     5521 gcagcgcttc gctgttcagc tccactcgct gtcgccgccc gagatcgtgc acatcgcggg
     5581 agtctcgggg ctgaacacgg ccgacacact ccggcggctg cgcgatgcgg gactggactc
     5641 gttgcctggc ggcggggccg aggtgctctc cgaccgagtg cgacaggcgg tgagcccggc
     5701 caagtgctcc gccggcgagt ggcacacagt gatggagcaa gcccacgccc tgggcatgcc
     5761 gaccaccgcg acgatgatgt tcggccacgg cgattcgccc gccgaccgcg tcgaccatct
     5821 gctggccgtg cgagccttgc aggatcggac cgggggcttc acggccttca tcccctggac
     5881 cttccagcca gcgaacaccg ccatggccca tcggcccaca ctcggcggac acgagtacct
     5941 cagaaccctt gccatcagcc ggctggctct ggacaacgtg cgaaacatcc aggcttcgtg
     6001 ggtgacccag ggggcgaagg tggctcagct ggccctgcgg tttggtgcca acgacatggg
     6061 atcgacgatg atggaggaga acgtggtgcg cgcggccggc gtcagcttcc ggctcgaccg
     6121 gcgcggaatc agttccctca tcactgcggc gggcttcgcg gcgcgccagc gtgacacccg
     6181 gtaccggcgg atcgggggct aggggtgaac gcacgagttc tcgccgtcat cggcggcagc
     6241 ggcgcctatg acctgctgcg cgatcagtcg ctcgaggggc cgtgcgccgg ccccgtcgag
     6301 accccctatg gcaacagcgc gcccgtgcgc cgaatcgact gcggtgaggg cgcggccgtg
     6361 atgttcatgt caaggcacgg cgaacgcggc tacgaggtgg cggcgccctt cgtcaactac
     6421 cgcgccaaca tctatgcgct caagagcctg ggcgtctctc atatcgtggc atggagcggt
     6481 cccggggcca ttgacccgag cctcgaggtc ggccagtacg tggtgcccga tgacctgctc
     6541 gatgcgacgc gcggccgagc cagcacgttc ttcgaaggca aagggcatgg cttcatccgt
     6601 cagaatcccg tgttctgccc cgtgctccgc agtctccttc tctcggcctg cgcctccacg
     6661 agccgaccgg ttcgggatgg cgccacctat gtgtgcaccc aggggccccg gctcgaaaca
     6721 ccggccgaga tccggcagta cgccattctg ggagcgcacc tggtggggat gacactcatc
     6781 cccgagagct tccttgcccg cgaactcgct atgggctatg cccccctctg ctacatcacc
     6841 aactacgccg aaggggtgcg cgagcggccc tacgagtccg gcacgctgtt cgagggactg
     6901 gcgaccaccg acgagcggca gcgtgtccga gatgccgtgt cggctctccc cgacctgctg
     6961 aggagcgtgg cccgcgcctg gcgtgagata gcgcccgact gcgcgtgcca cacctcgatg
     7021 gaacgctttg tgcggagtgg aaggctcggg cccgactggc ggacctggac cgatcgctag
     7081 gggggctacc cgggctcgcc gataatgtcc tccgcggccg catcgtcgtc gaggaacacc
     7141 cgccggaaga accgcactgc ccggctactg gtaagtatgg acaatacagc gccgccgaac
     7201 acgatggtct cgagcgcgat caggaagccc cggtcgaggc gtttggctga ggacgtgacc
     7261 cgcacgctcc tcggctcatc acggtcgagg tccgacacct tccacgtgaa caggaccgtg
     7321 tcgtcgctct cctgcgtgcc gcggaaggtc ggcgagtagc tcgtaatctt gcccggcatc
     7381 ttcacccgga acgcgatctc cgtgttggca aagtccttcc gaacgctcgg cacgggcagg
     7441 aaacgcgcca ggtccagggt ctccgtgtac gtgaacgact gctccgagct cgtggactgc
     7501 aggtcgagcg cgacgcccgg gtagtcgtcc accgatgcac gagagcgcga tgacgcgatg
     7561 gtgaagaccg tctgcgagtt ccgggtactc tccgagatct cccactcgcg gccgaaccgc
     7621 ttggcgatct cgatgcgcag cgactcg
//
LOCUS       DAOSPL010000021         7611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_9641_length_7611_cov_2.796718, whole genome shotgun sequence.
ACCESSION   DAOSPL010000021 DAOSPL010000000
VERSION     DAOSPL010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9641_length_7611_cov_2.796718"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="PLQ54_00775"
     CDS             <1..1201
                     /locus_tag="PLQ54_00775"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOS91821.1"
                     /translation="PVEVIDEHYYNTPEWFAANADRYDSYDRSGPRVYVGEYAVTQGC
                     GQGNLRAAVAEAAFMTGMERNSDIVVMASYAPLFVHVSKRTWNPDAIVFDSVSCYGTP
                     SYHVQRLFGRNRVDVVLPTDLVCGDSSVVEPGRIGLATWDTQAEFREVRVTHGDRVLY
                     AADFAQGTGGFTPQAGEWSIRDGAYRQTGTIQFASASAGDTSWVDYTYSVKARKLSGA
                     EGFLIKFRERDDANWYWWNIGGWGNRRHAIERSAGGNKTTLGDPVDGHVKTGHWYDIR
                     IELAGPRIRCYLDGRLIHDVVDKGPRIMAASAGWDESGGELVVKVVNLSDAPQVTRTT
                     LAGARRLDRRGRALVLTSNSPDDENSLARPDRVAPVELDVSIPGPDFTRTFPPHSLTI
                     LRIGAKD"
     gene            1273..2319
                     /locus_tag="PLQ54_00780"
     CDS             1273..2319
                     /locus_tag="PLQ54_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91822.1"
                     /translation="MVCSVRVCGIGALCALSLLLPSCMPVTDGGGGGGGGGGGGGEAP
                     TVTLSVEPTSLGISQGHVEFTWATTNATDIVSTNFGIDAGPLGASGSGRAVKWVTIAG
                     TQATFTITVRNAAGQTATAEAVVTVRGDAVNSPPHARCSNAVSSGDIASTIWSFDPTE
                     SWDEEQPPDTITMRFDWTGDGVFDTNWAPLAVTDHVFTEAEARACPPVPVTPPATAQE
                     NYRATVKMQVRDAAGATATWTQGLTLVIHSTNQAPEPAFTVTNPNGTFPNGFVDASAS
                     TDNEDGLGGLRFWWDWNFQGEDFPEPLLWTGWSDDPTATPPAYAGPGTYTILLAVMDS
                     GGLVNWTTRQIVVP"
     gene            complement(2369..3520)
                     /locus_tag="PLQ54_00785"
     CDS             complement(2369..3520)
                     /locus_tag="PLQ54_00785"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOS91823.1"
                     /translation="MRRRQPPPGNGDDLSGAFAELAERQERAMRIRRRLRWPAWLDRR
                     VVAVLGIAVVALISDGVRRENREFSASLDSVSGSAMVHRPGGAPEPASPGQVLSSGDV
                     VTTGLASRATIRYPDGSVLTLGGDTTLEVRLIEYNRGGGWRDRSIALRSGQCWTTAGP
                     GFGNGSELKVHTPSVVAAVRGSTISVAHDAISQVSRVCCSEGEVLIWGRIGSVSLSAG
                     TESSAVAGGPPAMAATIGEDAAGEFARHTELRVPPAKNPLLRRVESDLNRFLDPLLTV
                     LGIGRCSWGVGSSNSARRTGALESMRRIHTALQSFSTFPETLNPTTLEELGLGADVAK
                     RTVASFRGNAIDSYECDEPASAFRVTARAKDRASTPIVATTAGVHLGTP"
     gene            complement(3839..6565)
                     /locus_tag="PLQ54_00790"
     CDS             complement(3839..6565)
                     /locus_tag="PLQ54_00790"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOS91824.1"
                     /translation="MWTCMRGYWLTMVMLAMAPCVADEQLPVIAIPETGNPPSIDGTI
                     SEGEWSRAAVTCGFRDITTGRPGRAGSIVSLCYDGRALYVLFECEGEGAMAGGRGGER
                     DSAIWNGPLAELFLAPADWPVTDYAHFMVAADSTIADERVSAVGRDLSWNPEWRAATA
                     PAGDGWVAELAIPWEPLSVDATRSGTVFRACMARNAPSLDELSAWSPVRGGFHDPERF
                     GTLRLAGGEPVVAVRALPGRQLGETELTLRRTGSRRGLRVRVRGQSSRGALTLADTEL
                     RAGAEARAHRVTFDVAGGTEWLEVEVTSPDGSVAWRQTAGLALPDLAAVAAALRRRLE
                     GSGVPAGDRQRLGARLSPIVDQADGPLDEAGIDAATAELDAVARETTDSVGLADARAM
                     AGGNVTFYVTNPIVTRKIQPDTADPGPVARSLYIDMARNEFEPVQIVVCAVAEPLASV
                     RVSAGGLRGPGEAQIPAGRVTVSPIGFVRSAVRTPGASLEGELPDVLLPDRPMEVAKG
                     RRQPFLITIRTTDDDQPGEYRGVVRVRTTGGSVDIPLTVRVYDVAIPTKSTLRTAFVL
                     WGHFARFVSEPGPEAYFETYLRYSEVMLEHRISPITMWDPRKDAEGRWDFSDYDRLLT
                     ALVPKGLTTVNLGGNGRVCGDRNTEFVRAAEEHLKERGWWDLHYIYGHDEAAAGSLGE
                     LQANYKALVDAVPDLKIMQTGWNPTPGLEGLVRIWCPLTALADMNAIRQAQARGDEVW
                     WYVCCGPVAPYANLFVDYAGIDHRILGWQTFRYGIEGLLYWGVDVWPGNEQPLEEYDH
                     NNYAQWNPNSFGSFNGDGYLMYPGRNGEPLASLRLALLRDGLEDYELMARVRALATGK
                     GDLAARARRLLMLDAPLITSLVEYARDGALLLERRQAILEVGEALGQRGSGP"
     gene            complement(6575..>7611)
                     /locus_tag="PLQ54_00795"
     CDS             complement(6575..>7611)
                     /locus_tag="PLQ54_00795"
                     /inference="COORDINATES: protein motif:HMM:NF015390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOS91825.1"
                     /translation="RVGGEEGALLFDVVGRERQVVAHEGSVELEVTASAQYVVGIDPS
                     FLQRLLPPPRLPGKRQRLDPSKTIVVAYAENAPIDKDRNAYTVQPSEPLTFVIDTYNM
                     DERRSVTGRVRLEPPNGWTTDVGRLDISLGPMGRHVERITIRPTAGVGPAPVQVTVRG
                     EFGGEPIAPSVSYLRMDLTHAEPVARESLGLDAKDGWSSNISSNGTMSVADLPGGGVS
                     FSIRFDEPGDRWAYPAAKLAGIVDWSRWHGIAFEYRLDTDDPGTTGRVQVIEDSGASY
                     ISGGLSATRDWRRAVVLFADLGWGSFSTPDPNGRLDLTAVKGLLVGCNTAIDELTLQV
                     RNLELVAYGK"
BASE COUNT         1250 a         2675 c         2487 g         1199 t
ORIGIN      
        1 gcccgtcgag gtcatcgacg agcactacta caacacccct gaatggttcg ccgccaacgc
       61 ggatcggtat gactcctacg accgatctgg gccacgtgtc tacgtggggg agtacgcggt
      121 aacccaaggg tgcggtcagg gtaacctcag agcagccgtg gccgaggccg ccttcatgac
      181 gggcatggag cgcaactcgg acatcgtcgt gatggcgtcc tacgctccgc tgttcgtgca
      241 tgtgagcaaa cggacgtgga acccggacgc catcgtcttt gacagcgtgt cgtgctacgg
      301 caccccgtcc taccatgtcc agcgtctgtt cggtcggaac cgggtcgatg tcgtgctgcc
      361 gacagatctg gtctgcggtg actcgtcggt cgtcgagccg ggccgcattg gtctcgccac
      421 ctgggacacg caggccgagt tcagggaggt ccgcgtaacg cacggcgacc gggtgctcta
      481 cgccgccgac ttcgcccagg gcaccggcgg gttcacgccc caggcgggcg agtggagcat
      541 tcgtgacggg gcctaccggc agacgggtac gatccagttc gcctcggcct cggcgggcga
      601 cacctcgtgg gtcgactata cgtactcggt caaggcgcgg aagctcagtg gtgccgaggg
      661 gttcctcatc aagttccgcg agcgcgatga tgccaactgg tactggtgga acatcggcgg
      721 ctggggcaac cggcgccacg ccattgagcg atcggctggt gggaacaaga cgacgctggg
      781 cgatccggtg gatggtcacg tcaagacggg gcactggtat gacattcgca tcgagctcgc
      841 cggccctcgc atccggtgct atctggacgg taggctgatc catgacgtgg tggacaaggg
      901 cccgaggatc atggcggcga gtgccggatg ggatgagtcc gggggcgagc tggtcgtgaa
      961 ggtggtcaac ctgtcggatg cgccgcaggt gactcggacg acgctggccg gggcacgccg
     1021 actcgaccgg cgcggtcgcg ccctcgtgct cacctcgaac tcgcctgacg acgagaactc
     1081 gctcgcccgg cctgacaggg tggcgcccgt cgagctggac gtgagcattc ccggccctga
     1141 tttcacacgg acgttcccgc cgcactcgct gacgatcctg cgcattgggg cgaaggactg
     1201 aaggacggag ccgatcgggc cctggcgtca gatggcgcca gcccgcagtg atactgagga
     1261 ggactgatag ccatggtctg tagcgtcagg gtctgtggta ttggggcgct gtgcgcgctc
     1321 tcgctgttgc tgcccagttg catgccggta acggatgggg ggggcggggg cgggggcggg
     1381 ggcgggggcg ggggcgaagc cccgaccgtc acgctctccg tagagcccac gtcgctgggc
     1441 atcagccagg ggcatgtgga gttcacgtgg gcgacgacga acgccacgga catcgtgagc
     1501 accaacttcg ggatcgatgc cggccccttg ggcgcgagcg gttcgggccg cgccgtgaag
     1561 tgggtcacga ttgcgggcac ccaggccacc ttcaccatca ccgtccggaa tgcggcgggc
     1621 cagaccgcga cggccgaagc tgtggtgacg gtccgcggcg acgcggttaa ctcaccaccg
     1681 catgcccgct gctccaacgc cgtgagttcg ggcgacattg catccacgat ttggagcttc
     1741 gaccccaccg agtcctggga cgaagagcag cccccggaca ctatcacgat gcggttcgac
     1801 tggacgggtg acggcgtctt cgacaccaac tgggctccgc tcgccgtgac cgaccatgtg
     1861 ttcaccgagg ccgaggcccg agcctgtccg cccgtccctg tcacgccgcc ggccacggcc
     1921 caggagaact accgtgcgac cgtgaagatg caggtgcgcg acgctgccgg ggcgaccgcc
     1981 acgtggaccc agggtctcac gctggtaatc cactcgacga accaggcacc cgagcccgcg
     2041 ttcactgtca cgaatccgaa tggcacattc ccaaatgggt tcgtggatgc ctcggcctcg
     2101 accgacaacg aggatgggct gggtggtctc cgcttctggt gggactggaa cttccagggc
     2161 gaggacttcc ccgaaccgct gctgtggacc ggatggtcgg atgacccgac ggcgacaccg
     2221 ccggcctacg cgggccccgg cacctacacg atcctgctcg ctgtcatgga cagcggaggt
     2281 ctggtgaact ggaccactcg gcagatcgtg gtgccgtaga cgctattggg ccggaggtgg
     2341 gccatcccgc ctccggccgg cagttggcct acggtgtgcc caggtggacg ccggcggtgg
     2401 tggcgacgat gggtgtcgac gcccggtcct tggcccgcgc cgtcaccctg aaggcactgg
     2461 cgggctcgtc gcactcgtac gagtcgatcg cgttgcccct gaagctggcc acggtacgct
     2521 tagccacatc ggcgcccaag ccgagctcct cgagcgtggt ggggttgaga gtctccggga
     2581 acgtgctgaa cgactgcagg gccgtgtgga tccggcgcat ggactccagt gcgccggtgc
     2641 gacgcgccga gttgctactg ccgactcccc aactgcagcg accgatcccc agcacagtga
     2701 gcagtgggtc cagaaaccgg ttgaggtcgg attcgacccg acgaagcagc gggttcttcg
     2761 ctggcggcac gcgaagctcg gtgtggcgcg cgaactcgcc cgccgcgtcc tcgccgatgg
     2821 tcgccgccat ggccggagga ccgccggcta cggcgctgct ctcggtaccc gcgctgagcg
     2881 aaaccgaccc gattcgtccc cagatcagga cctcaccctc gctgcagcac acccggctga
     2941 cctgcgatat ggcgtcgtgg gcgacgctga tggtgctccc ccgcaccgcc gccaccacac
     3001 tcggtgtgtg caccttcagc tcagacccgt tcccaaaccc cggtcctgcg gtggtccagc
     3061 actgaccgct ccgcagcgcg atcgaccggt ctcgccagcc gcctcccctg ttgtactcga
     3121 tcagccgtac ctcgagtgtg gtatctccac cgagggttag cacgctgcca tccgggtacc
     3181 gaatggtcgc ccgggaggcc aagcctgtgg tgaccacatc gcccgacgac agcacctgcc
     3241 cggggctcgc cggctcgggg gcacctccgg gtctgtgaac catggcgctc ccggacactg
     3301 agtccagcga cgcggagaac tcccggttct cgcgccggac gccgtcggag atgagcgcga
     3361 ccacggcaat acccagcaca gcgactactc gccgatcgag ccaggctggc catcggagcc
     3421 ggcgccgaat gcgcatcgcc cgctcctgtc gctctgcgag ctcggcgaac gctccagaca
     3481 agtcatcgcc gttacccggc ggaggttggc gtcgccgcat tcccttcgcc tccttcggac
     3541 ggaccaaggc ccgccaccga gaaggcgagc aagatcccca tggccggcac ataggccccc
     3601 cttgcgtcac aagccccgcg cgatggaacg gtccaccgaa cattgccgcg ccgatctggg
     3661 caaaggctat cgcggccgca acgggccccc gcacttcgaa gtggccgccc gaggctgaca
     3721 ccgactcggc gccactcccg gcaagcgcac ccgagcagtg ccacgagcaa ggacagcaac
     3781 ggctccgtct ggagtccgag ccggcgttcc cagagtggtc tgtccccccc ccgggccgct
     3841 agggccccga tccgcgttgg cccagtgctt cccccacctc gaggattgcc tggcgccgct
     3901 ccagcaggag cgcgccatcc cgtgcatact caaccaggct cgtaatcagt ggcgcgtcca
     3961 gcatcagcaa tctcctggcc cgggcagcga ggtcgccctt gccggtcgcc agcgcccgca
     4021 cgcgggccat cagctcgtag tcctcgagcc catcgcggag cagggcgagt cggagcgacg
     4081 cgagcggctc gccgttccga cccggataca tgagatagcc atcaccgttg aacgagccga
     4141 aggagttcgg gttccactgt gcgtagttgt tgtggtcgta ctcctcgagg ggctgctcat
     4201 tccccggcca gacgtcgacg ccccagtaca ggagcccttc gattccgtag cggaacgtct
     4261 gccacccgag aatgcgatga tcgatgcccg catagtccac gaacagattc gcgtagggcg
     4321 ccaccggccc gcagcagaca taccaccaca cctcgtcacc tcgggcctgg gcttgccgga
     4381 tggcattcat gtcggcgaga gccgtcagag gacaccagat gcgtaccagg ccctcgaggc
     4441 caggagtcgg gttccacccc gtctgcatga tcttcaggtc gggcacggcg tccaccaggg
     4501 ccttgtagtt ggcttggagc tcccccaggg agcccgccgc ggcctcatcg tgcccgtaga
     4561 tatagtggag gtcccaccag ccgcgctcct tgaggtgttc ctccgccgca cggacgaact
     4621 cggtgttccg gtcgccgcac acgcggccgt taccccccaa gttcaccgtg gtaaggccct
     4681 tgggcaccag cgcggtcagc aggcggtcgt agtcggagaa gtcccatcga ccttcggcgt
     4741 ctttccttgg gtcccacatc gtgatcggac tgatgcggtg ctcgagcatc acctccgagt
     4801 agcggaggta tgtctcgaaa taggcctcgg gacccggctc gctgacgaac cgagcgaagt
     4861 ggccccagag aacgaacgcc gtgcgcagcg ttgacttcgt cgggatcgcc acgtcgtaga
     4921 cgcgtaccgt gagggggatg tcgaccgagc ctccggtcgt gcggactctc accacccctc
     4981 tgtactcgcc gggctggtcg tcgtcggtgg tgcggatggt gatgaggaac ggctgccgac
     5041 gacccttcgc gacctccatg ggccggtcgg gtagtagcac gtcaggcagc tcgccctcga
     5101 gcgatgcgcc gggggtcctc accgcgctgc gcacgaaccc gatgggcgat acggtcacgc
     5161 ggccggccgg gatctgggcc tcgccgggcc cgcgcagacc tccggccgac acgcgcaccg
     5221 acgccaaagg ctccgcgacg gcacatacga cgatctgtac tggctcgaac tcgttgcggg
     5281 ccatgtcgat gtacagcgac ctggcaacgg gacccggatc ggccgtgtcg ggctggatct
     5341 tccgggtcac gatcggattg gtaacataga aagtcacgtt gccgcccgcc atggcccgag
     5401 cgtcggcaag accgaccgag tcggtcgtct cccgtgcgac cgcgtccagc tcagccgtgg
     5461 ccgcgtcaat gcccgcctcg tccagcgggc cgtccgcttg gtcgacgatc ggtgagagcc
     5521 gggccccaag cctctggcga tcgccggccg ggacgccgga accctccagg cgccggcgga
     5581 gggcggccgc gaccgccgcg aggtccggca gggccaatcc ggccgtctgc ctccacgcca
     5641 ccgatccgtc gggcgaggtg acctccacct cgagccactc ggtgccaccc gccacgtcga
     5701 aggtgactcg atgcgcgcgc gcctccgcgc ccgccctcaa ctccgtgtca gccagagtga
     5761 gcgcaccccg cgagctctgc ccccggaccc gcacacgtag cccgcgcctc gagccggtcc
     5821 gccttagcgt cagctccgtc tcgcccagct gcctgcccgg aagtgcgcgg acggccacaa
     5881 ccggctcgcc gccggccaga cgaagcgtcc cgaagcgctc cgggtcatgg aagcccccgc
     5941 gcaccggcga ccaggcgctc agctcgtcca ggctgggcgc attccgggcc atgcatgctc
     6001 ggaagaccgt gccggaccgg gtggcgtcca ccgacagcgg ttcccacgga atcgccagct
     6061 cggcgaccca gccatcgcca gcgggtgccg tcgccgcccg ccactccggg ttccagctca
     6121 gatcgcgccc gacggcgctc acgcgttcgt cggcaatcgt ggagtccgcc gcgaccatga
     6181 agtgggcgta gtcagtgacc ggccagtctg ccggagcgag gaacaactcg gccagcggtc
     6241 cattccagat cgcggaatcc ctctcgccac cccggccacc ggccatggcc ccttcgccct
     6301 cgcactcgaa gagcacgtag agcgcccggc cgtcatagca cagcgagacg atcgagccag
     6361 cgcggcccgg gcgcccggtc gtgatgtctc ggaagccaca ggtcaccgcg gccctggacc
     6421 actcgccctc actgatcgtc ccatcgattg acggggggtt cccggtctca ggaatcgcaa
     6481 tgaccggcag ctgctcgtcc gccacacacg gtgccatcgc cagcatgacc atcgtgagcc
     6541 agtagccgcg catgcatgtc cacattcccc atcctcactt gccgtacgcc acgagttcga
     6601 ggttccgcac ctgaagtgtc agctcgtcga tggccgtgtt gcagccgacg agcagcccct
     6661 tcacagccgt caggtccagc ctcccgtttg gatccggcgt cgagaacgat ccccagccaa
     6721 gatccgcgaa cagcaccacg gcccgccgcc aatccctggt cgccgacagg ccgccgctga
     6781 tgtaggacgc gcccgaatcc tcgatgacct ggactcggcc cgttgtccct ggatcatcgg
     6841 tgtcgagccg gtactcgaag gcaatgccat gccaacgaga ccagtcgaca atgcccgcca
     6901 gcttcgccgc gggatacgcc caccggtcgc ccggctcatc aaaccggatc gagaagctca
     6961 ccccgccgcc cggcagatcg gccaccgaca tggtgccatt gctcgatatg ttgctcgacc
     7021 acccatcctt cgcgtccagg ccaagactct cccgagccac aggctcggcg tgagttaggt
     7081 ccatccgaag gtacgacacc gatggcgcga tcggctcgcc gccgaactcg ccacgcacgg
     7141 tgacctgcac gggagccggg cccacgcctg cggtgggccg aatcgtgatg cgctccacgt
     7201 gtcggcccat cggcccgagg gagatgtcga ggcggccgac gtccgtcgtc cacccattgg
     7261 gcggttcaag acggacacgc cccgtcaccg aacgccgctc gtccatgttg taggtgtcga
     7321 tcacgaaggt cagcggctcg gacggctgta ccgtgtacgc gttccggtcc ttgtcgatgg
     7381 gtgcgttctc ggcgtaggcg accacgatgg tcttgctcgg atcgagccgc tggcgcttgc
     7441 cgggtaggcg cgggggcggc agtagccgct gcaggaacga agggtcgatg cccaccacgt
     7501 actgagccga ggccgtgact tcgagctcca ccgatccctc gtgcgccacc acctggcgtt
     7561 cgcggccgac gacatcgaaa agaagcgcgc cttcctcgcc gccgacccgg t
//
LOCUS       DAOSPL010000022         7576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_9695_length_7576_cov_2.995080, whole genome shotgun sequence.
ACCESSION   DAOSPL010000022 DAOSPL010000000
VERSION     DAOSPL010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7576
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9695_length_7576_cov_2.995080"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PLQ54_00800"
     CDS             complement(<1..757)
                     /locus_tag="PLQ54_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOS91826.1"
                     /translation="MAGAPPYVLGVDCGTQSLRSALFDLAGNPVAQAVEGFETTYPQV
                     SWAEQNPEDWLRAMTHTVRDCLSQAGIRPEDVCGIGLDTTACTPVAVDDHGTPLRPAL
                     LWMDQRAHREAAVLSATGHPILRYAGGPVSPEWGIPKSLWLKANEPETYRRAARIVDC
                     SEFLTFHLTGNWVASLNTLTCKWNYARLDGGYAGDLLESLGLADWRTKYVQDVRPVGD
                     RAGELTPIAAERLGLNAGTPVAVGGIDAYMGMIG"
     gene            893..1093
                     /locus_tag="PLQ54_00805"
     CDS             893..1093
                     /locus_tag="PLQ54_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS91827.1"
                     /translation="MELETSDGQAGGEQIRLSDLIREKRWDEALRLLEEALAVRPRDP
                     ALWYHRGCALAHLGRLAEAEDA"
     gene            complement(1202..2923)
                     /locus_tag="PLQ54_00810"
     CDS             complement(1202..2923)
                     /locus_tag="PLQ54_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOS91828.1"
                     /translation="MRLCLGVFCALLAWGGIDAVAQAPAAADRPLAGKTIVVDPSHGL
                     KDAAGTVISAGAVGPGGVSEAEVVLRVAEELRVRLESFGAAVVLTRSVAQPWRIGDDP
                     RDDAMARARSAAQCHAAAFVRLHADAATDPSTRGPVVLFFTDESRALAHAIFDALTQP
                     ASGQGVFKRYDYGLEACTAPAVSVGLGVVSNPHDERVLATDTFQKQAAQAISQALVMA
                     LTTPAPAEHGAAPPLVSDPEGELGEENVDDTGTARVLAQCSQRLLQSVLAITNRTPWH
                     VGLVARNLATGEATWIRPDTPFPAAQLVGLPVMAETTRQIREGKLKPGVAPPGGTRTV
                     FQLTQAMIIGGDLTAADALIGLLGLDSIDSTASLLGMTATSVQRKPGDVAAKQRGLEN
                     LTTPDDLARMLEQVAIGQSVSGEASKAMYEMLRRHANRQLIPAGVPAEAEVANLTASQ
                     PGLIADAAIVRTPFTTYVLVVFLRDVEGRAGGPDPAGLIPEVSRAVYEHFARTYLAVD
                     TRPPGLKVFVNDRLKGIAPCLIQITSATPQAQEFTIAARADDGRTARRVVVVSQGDRQ
                     AVQLSLD"
     gene            complement(3078..3677)
                     /locus_tag="PLQ54_00815"
     CDS             complement(3078..3677)
                     /locus_tag="PLQ54_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018734054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS91829.1"
                     /translation="MPGRLEITGLHRPSAGSHGRDEFMRQADRCIRQVRATALRMTKN
                     PDDAEDLVQDALVKAYASFDRFQSGTNFKAWLFRILTNTYINEFRRRKRSPDLVPMDD
                     LEPSAEYDMSVEHGSTELLPEDLLMAKIPDENIQAALANLPDEFRIAVTLCDVGELTY
                     EEVAAAMQTPIGTVRSRVHRGRRMLRRQLESYAKAQGWV"
     gene            complement(3880..4350)
                     /locus_tag="PLQ54_00820"
     CDS             complement(3880..4350)
                     /locus_tag="PLQ54_00820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOS91830.1"
                     /translation="MTIAAPVVIRPIRGRELVAVHRISSRAFEIPYSYETLINYRHRA
                     RGGLLVAEVAGEIVGFVVATSPLISLWGGKVGEIAVLAVEPDHRGQGIGSRLLRAGLD
                     TLKARGMSSARLHVETGNVRAIALYERFGFTREARVTAYYRNGKDAYRMIKSLE"
     gene            4521..5579
                     /locus_tag="PLQ54_00825"
     CDS             4521..5579
                     /locus_tag="PLQ54_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS91831.1"
                     /translation="MAKHGFALIGLGAVSGLHADAIQQLPKAELRAVCDINEDRARTV
                     AAQRGCDWTTDHRALLNRPDITAVDITTWSGLHGPIGTEFARAGKHVITTKPIDVRLE
                     VIDELIEACDTNGVKLAAVHQSRALEPYCRLKAAIDQGRLGRILMGHAYCKWLRTQAY
                     YDSSAWRGTYELDGGGCLMNQGIHYVDLLLWYLGQPVTVAGHTATLCRRIEVEDCATA
                     SLRFASGALGTIEAATCLYRGQPARVEVHGENGNVAIAGDEVVWWDVEGEETIHRPPG
                     AIDGSSDPAGGMVNALDAHIAQIGDFVRAIDEDREPELSGREARRAVEVILAIYRSSR
                     TGQVVTIPLKGNSDPRAV"
     gene            complement(5601..>7576)
                     /locus_tag="PLQ54_00830"
     CDS             complement(5601..>7576)
                     /locus_tag="PLQ54_00830"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91832.1"
                     /translation="RPIPLVVNLHGWDSGNGPFAFPSGSYGPEKDLVGDRGWLMVYPF
                     GRGNQDWRDAGEVDVREVIAEVSRRFPVDASRIYLVGFSMGGRGVWHLGMRYPSDWAA
                     IVPIAGGSAGSVWGVWNEERLVTNLSQTPVFAVHGTRDEIVPVAGSRVLERQLRRILS
                     PDFEYRERPFASHESMPDLAKEWVPWLERHALGRPPSRVSYATDSARFGRAFFVRVVQ
                     LEREGRTGSVAAHVAEGNVVDLLVDGVNRLSLDLGAHPAIRAAQPVTVSVNRSSVLRV
                     TLPPSGTLDLSSAGPGAWALADGPVPAEEKRAGFCGPMRDALARPFAIVYGTQGADAA
                     LVAASYSMAQDAARAWREWWWGVPRVVRDADVTPELMGSHNLLLFGGEFVNGVVAQLA
                     AQMPFRDGRPCPLAPGVDPRDVAYKFIYPNPEAPGRYVVVMAAQDPVALQAIGQLGQK
                     DPYPAPLGTLDVDYIVAVRRCIGSTVTLAPVAEGWFDNHWRFQPAAPGHMESTGRSWL
                     ASVSPQTGWESAMVADASFGPTLVMTRTATDNEAWRPFELRSYHDVLHRFGANTAVLW
                     APGDPPPEVCWFRRTLTLAQAPVRAPARVLMDDRGRLFVNGQLACATSTDEGIIELDL
                     APWLRAGENVLAAEVANLYGARGFAFEWAVSEH"
BASE COUNT         1320 a         2648 c         2464 g         1144 t
ORIGIN      
        1 ggccgatcat gcccatgtag gcatcgatgc cgcccacggc cactggcgta cccgcattga
       61 ggcccaggcg ttcggccgcg atgggagtga gctcgcccgc ccggtcaccc acgggccgca
      121 catcctgcac atacttggtc ctccagtcgg cgaggcccag gctctccaaa aggtcgcccg
      181 cgtagccacc gtcgagtcgg gcgtagttcc acttgcaggt cagcgtgttg agtgaggcca
      241 cccagttgcc tgtgaggtgg aaggtcagga actcggagca gtcgacgatg cgcgccgccc
      301 ggcggtaggt ctccggctcg ttggccttaa gccagagcga ctttgggata ccccactcgg
      361 gcgagaccgg gccgcccgca taacggagga tggggtgccc cgtcgccgag agaacggcgg
      421 cctcacgatg cgcccgttga tccatccaga gcagcgctgg gcgcagcgga gtcccatggt
      481 catcgaccgc caccggcgtg cacgcggtag tatcgagccc gatgccgcac acgtcctcgg
      541 gccgaatccc ggcctggctc agacagtcgc ggaccgtgtg ggtcatggcc ctgagccagt
      601 cctcggggtt ctgctcagcc cagctcacct gcgggtatgt tgtctcaaag ccctcgaccg
      661 cctgggcgac agggttaccg gccagatcga agagggccga gcgcagagac tgagtgccgc
      721 agtcgacacc gagcacatac ggaggtgctc ccgccattcc cattccctcc gggactacgc
      781 tagtctggtg ggcggctgct tcgccagccc cagggccccc tcctcgtcgg ggctccggga
      841 tgagcggccg ggccgaaggg acgcgccctg gcatcgagag gaggccccac ctgtggagct
      901 agaaacgtcc gacggccagg cgggcggcga acagatccgt ctctcggacc tcatccgcga
      961 gaagcggtgg gacgaggcac tccgactgct cgaagaggcc ctcgccgtcc gtccgcgcga
     1021 ccccgcgctg tggtaccacc gcggatgcgc gctggcccac ctgggaagac tggccgaggc
     1081 cgaggatgcc tgacgcactg cctcacagag cgccccggga gccgagcggc gcagtgcctg
     1141 ctcgcgacca tcagtgccct acggcgcacc gagggagccg cagaagaccc ccccgcgccc
     1201 atcagtcgag tgagagctgc acggcctgac ggtcgccctg actgaccacg acaactcggc
     1261 gggccgtccg gccgtcgtcg gcccgcgcgg caatggtgaa ctcctgggcc tggggcgtgg
     1321 cggacgtgat ctgtatcagg cagggcgcga tgcccttgag ccgatcgttc acgaacacct
     1381 tcaagcctgg tggacgcgtg tcgaccgcca ggtaggttcg ggcgaagtgc tcatagacgg
     1441 ccctggacac ctccgggatc aatccggcgg ggtccggacc accggcccgg ccctccacat
     1501 cccggaggaa caccacgagc acgtacgtgg tgaacggcgt ccgcacgatg gcggcgtcag
     1561 caatcagccc gggctggctc gcagtcaggt tggcaacctc agcctcggcg ggcacccccg
     1621 cgggtatgag ctggcgattc gcatgccgcc gcagcatctc atacatcgcc ttcgacgcct
     1681 cgccactcac gctctgtccg atggccacct gctccaacat ccgggccaag tcgtcgggcg
     1741 tcgtcaggtt ctcgagacct ctttgcttcg ccgcgacgtc gccaggcttg cgttggacgg
     1801 acgtggccgt cattccgagt agcgaggccg tcgagtcgat gctatccagc cccaggaggc
     1861 cgatcagagc atccgcggca gtcaggtcac ctccgatgat catggcctgc gtgagttgga
     1921 agacggtccg ggtcccccct ggcggtgcga cccccggctt cagctttccc tcgcgaatct
     1981 ggcgcgtggt ctcggccatg accggcaggc ccaccagttg agccgcaggg aatggagtat
     2041 cgggccggat ccaggtcgcc tcgccggtcg ccaggtttct ggcaaccagg ccgacgtgcc
     2101 atggagtgcg attcgtgatc gcgagcacgc tctggagcag ccgctggctg cactgggcaa
     2161 ggaccctggc cgtgccggta tcgtccacgt tctcctcgcc gagttcgccc tcggggtccg
     2221 aaaccaaggg aggcgctgcc ccgtgctcgg ccggcgccgg cgtggtcagc gccatgacca
     2281 gggcctggct gatggcttgg gccgcctgct tctggaaggt gtccgtggcc aagacgcgct
     2341 cgtcatgtgg gttcgacacg acgcccagcc cgacggacac ggccggagcg gtgcaggcct
     2401 cgagtccgta gtcatagcgc ttgaacacgc cctggccact ggcgggctgg gtcaacgcgt
     2461 cgaagatggc gtgggccaga gctcgactct cgtcggtgaa gaagagcacc actggcccac
     2521 gggtcgacgg gtcggtcgcc gcatcggcat gaagccggac gaaggcagct gcatggcact
     2581 gagccgccga acgcgctctg gccatggcat catcgcgcgg gtcatcaccg atgcgccaag
     2641 gctgggccac cgaacgcgtg agcaccaccg cagctccgaa ggactcgagc cgcacccgaa
     2701 gctcctcggc cacccggagg acgacctcgg cctcgctgac cccgccggga ccgacggcac
     2761 ccgcgctgat gaccgtgccg gccgcgtctt tgaggccatg ggaagggtca accacgatgg
     2821 tcttcccggc caatgggcgg tcggccgcgg cgggcgcctg ggcgacggcg tcgatgcctc
     2881 cccaggccag cagcgcacag aatacgccca agcatagccg catggccgcg acaccacctc
     2941 cggccgcttc ccggctcagc gagttcgccc ctactcggtt gacgtcttcg gggtgagtct
     3001 cgttcccact gggcccaagg accacaacgg ccggccacgc tgcgagggtc aaaccagcgc
     3061 gaacgtccgg tgcctgccta gacccagccc tgagctttgg catagctctc gagctgccgt
     3121 cgaagcatgc gccggccgcg gtggacacgc gaccgcaccg tgccaatggg cgtctgcatg
     3181 gcggcggcca cctcctcgta ggtgagctcg cccacgtcgc acagcgtgac ggcgatgcgg
     3241 aactcatcag ggaggttggc cagcgccgcc tggatgttct cgtcagggat cttcgccatg
     3301 agcagatcct cgggcaggag ctccgtcgac ccatgttcga cgctcatgtc gtactcggcc
     3361 gacggctcga gatcatccat gggcaccagg tccggcgagc gcttgcggcg ccggaactcg
     3421 ttgatgtagg tgttggtcag gatccggaac agccaggctt tgaagttggt gccggactgg
     3481 aatcggtcaa aggaggcgta cgccttcacg agcgcatcct gcacgaggtc ttcggcatca
     3541 tcggggttct tggtcatccg taacgccgta gcccgaacct ggcgaataca gcggtcggcc
     3601 tggcgcatga actcatcgcg gccgtggctg cccgccgacg gccgatgcag tccggtgatc
     3661 tcgagacgcc ctggcaagat accacgatcc ctgagttgcg gccgaagtcg gcctcccgtg
     3721 agacaggccc tgtgggctgc cacgccgggt caaccatcgg gtccacccag tgacggccga
     3781 ccgcgcaacc gcccccgggg tattcgaccc gtgagcggac tcgcccttcg gcgcgccgca
     3841 gcccaagccg agcttcctcg acccgggcat gacgccccac tactcgaggg acttgatcat
     3901 gcgatatgca tccttaccat tgcggtagta ggcggtaacg cgggcctcgc gcgtgaaccc
     3961 gaagcgttca tacagggcaa tcgcgcgtac gtttccggtc tcgacatgca gccgggctga
     4021 cgacatgccc cgggccttga gtgtgtccag gccggcccgg agcagccgag agccgatccc
     4081 ctgaccccgg tgatcgggtt ccacagccag tacggcgatc tcgcccactt tgccacccca
     4141 gaggctgatc agcggactcg tcgccaccac gaaacccacg atctcgcccg cgacctcggc
     4201 caccaacaga cctccacgtg cccgatggcg atagttgatg agggtctcgt aggagtaggg
     4261 tatctcaaat gccctggacg agatgcggtg gacggcaacc agctctctgc cacggatggg
     4321 gcggataacg acgggagctg cgatggtcac gcggtcccag cccttcagtg gtttcgtttc
     4381 ctaacgtgca ggtaccggcc cccggttcgc gggcacggcg ggcgagccag gattcggtcc
     4441 caagtgcgcc gaatgagtcc ccccggcact cagggagcac cccgcgacac gggccacctg
     4501 gacctacgga cggagatgca atggcaaagc acggcttcgc cctcatcggg cttggtgcag
     4561 tgtccggact gcacgccgac gcgatccaac agctccccaa agcagagtta cgtgctgtgt
     4621 gtgacatcaa tgaggatcga gctcggaccg tcgccgccca gcggggttgc gactggacga
     4681 ccgaccatcg cgctcttctg aaccggcctg acatcaccgc cgtcgacatc accacgtggt
     4741 cgggcctgca tggccccatc ggcaccgagt tcgcccgtgc gggcaaacac gtcatcacga
     4801 cgaagccgat cgacgtgcgg ctcgaggtca tcgatgagct gatcgaggcg tgcgacacca
     4861 acggtgtgaa gcttgcggcc gtccaccaga gccgcgctct cgagccatac tgccggctga
     4921 aggcggcgat cgaccaaggc cgcctcggcc gcatcctcat ggggcacgcc tactgcaagt
     4981 ggctgcgcac ccaagcctac tacgacagca gcgcctggcg gggcacctat gagctcgatg
     5041 gtggtgggtg cctcatgaac cagggcatcc actatgtgga ccttctgctg tggtacctgg
     5101 gccaacccgt caccgtggct ggacacaccg ccacgctctg ccggcgcatc gaggtcgagg
     5161 actgcgccac ggcctcccta cggttcgcca gcggcgccct gggcaccatc gaggcagcca
     5221 catgcctcta tcggggccag ccggcgcgtg tggaggtcca cggtgagaac ggcaacgtcg
     5281 ccatcgccgg cgacgaggtc gtatggtggg atgtggaggg cgaggaaacc atccaccgcc
     5341 cgcccggcgc catcgacggt tcctccgatc ccgcaggcgg catggtcaac gctctggatg
     5401 cacacatcgc gcagatcggc gatttcgttc gtgccatcga cgaagaccgc gagcccgagc
     5461 tgagtggacg cgaagcccgg cgcgccgtgg aggtgatcct ggcgatctac cggtcgagcc
     5521 gcacgggcca ggtggtgacc atccccctga agggcaactc cgaccccagg gccgtgtgag
     5581 cggtcgtgaa cccaggcctg tcaatgctcc gacaccgccc actcgaacgc aaatccccgc
     5641 gcgccataga gattcgcgac ctccgcggcc agcacattct cacctgcgcg caaccagggc
     5701 gccagatcga gctcgatgat cccttcgtcc gtgctggtgg cacaggcaag ctgtccgttg
     5761 acgaacagac ggccccggtc atccatgagg acgcgcgcgg gcgctcggac gggcgcctgc
     5821 gccagggtca gtgttcgccg gaaccagcac acttccggcg gcggatcgcc gggggcccag
     5881 agcaccgccg tgttcgcacc aaaccgatga agcacgtcgt ggtagctccg caactcgaag
     5941 ggccgccacg cctcgttgtc ggtggcggtc cgggtcatga cgagcgtcgg cccgaagctg
     6001 gcgtcggcca ccatcgcact ctcccaaccc gtctggggcg agacactggc cagccaactg
     6061 cgaccagtgg actccatgtg gccaggagcg gccggctgga accgccaatg gttatcaaac
     6121 cacccctcgg ctaccggcgc caacgtcacg gtcgagccga tgcaccggcg gaccgccact
     6181 atgtaatcga cgtccagcgt gcccaatggc gcagggtagg gatccttctg gcccagctgg
     6241 ccaatggcct gtagtgccac gggatcctga gctgccataa ccaccacgta gcggccgggc
     6301 gcctccgggt tcgggtagat gaacttgtag gccacgtcac gcggatccac acccggcgca
     6361 aggggacagg gtcggccatc tcggaacggc atctgggccg ccagctgcgc caccaccccg
     6421 ttgacgaact cacccccgaa caacaacagg ttgtggctcc ccattagctc gggcgtgaca
     6481 tcggcatcgc gaaccacccg tggaacaccc caccaccact cccgccacgc cctggccgca
     6541 tcctgagcca tggagtagct ggcggcgacc agggccgcgt ccgccccctg ggtgccgtag
     6601 acgatagcga agggccgggc cagggcatcg cgcatgggac cgcagaaccc agcgcgcttc
     6661 tcttccgccg gaacagggcc atcggccagc gcccaagcgc ccggtccggc tgaagagagg
     6721 tcgagtgtcc ccgaaggagg cagcgtcacc cgcagcacgg acgaccggtt cacgctgacg
     6781 gtcaccggct gggccgcccg gatggccggg tgggccccca ggtcgagcga gagacgattg
     6841 acaccatcga ccagcaagtc tacgacgttc ccctcagcga catgggcggc cacactgccg
     6901 gtgcggcctt ccctctcgag ctggacaacc cgcacaaaga aggcccggcc gaagcgggcg
     6961 gaatcggtgg cgtagctcac tcgcgatggc ggcctcccca gtgcatggcg ctcaagccag
     7021 gggacccact ctttggcgag gtcgggcatg ctctcgtggc tggcaaacgg gcgctcccgg
     7081 tattcgaagt ccgggctcag tatgcgccgg agctggcgct cgagtacgcg actcccggca
     7141 acaggcacaa tctcatcgcg cgtcccatgg acggcgaaga ccggggtctg cgacaggttc
     7201 gtgaccagcc gctcctcatt ccacacaccc cagaccgacc cggcagaccc cccggcaatg
     7261 ggaacgatgg ctgcccagtc ggaggggtac cgcatgccca agtgccacac cccgcggccg
     7321 cccatcgaga aacccacgag gtagatacgg ctcgcgtcga ccggaaaccg tcgcgacacc
     7381 tcggcaatga cctctcgcac atcaacctcg ccggcatcgc gccagtcctg gttccctcgc
     7441 ccaaaggggt agaccatgag ccagccgcga tcgcccacca ggtccttctc tgggccgtac
     7501 gaacccgacg ggaaggcgaa gggcccgttg ccgctatccc aaccgtgcag attgacgacc
     7561 agcgggatgg gccggg
//
LOCUS       DAOSPL010000023         7498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_9849_length_7498_cov_3.629988, whole genome shotgun sequence.
ACCESSION   DAOSPL010000023 DAOSPL010000000
VERSION     DAOSPL010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7498
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9849_length_7498_cov_3.629988"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLQ54_00835"
     CDS             complement(<1..204)
                     /locus_tag="PLQ54_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOS91833.1"
                     /translation="MPSAAEIDAFWERRLAVLAETPPEPRLVPAPEWSTDAVHTSRVT
                     LRSAGGVDVCGWLCVPRHGPRPLP"
     gene            complement(216..1211)
                     /locus_tag="PLQ54_00840"
     CDS             complement(216..1211)
                     /locus_tag="PLQ54_00840"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS91834.1"
                     /translation="MRIGVVGLAGIGAHHLRVFAGMNEFELVAACDINPAVLEERTRD
                     MAVARYSDAEAMMDRERLDAVSLCTPPKVHVPHTELAARRGIHVLVEKPMAPTPEACQ
                     VMIDACAAGGVVLMVAHKKRYVPAVARLRELIDTELGRPEVLYHRYPHPWMSEAEWFW
                     AEDDGGGPLLENAVHAADTVRFLLGDIERLYAEGDVFNAPRRAPQLNCAVYTVRATSG
                     AIGMIGAGMIGTPSPGLAFEDFYIACEHGTAQVSGDFDNPAHLRYALRARPAEVHSES
                     FTDGDPFAAEFRHFAECVREGKEPLTSGSEGKKAIELCMAVKQSARTGQPVVLPV"
     gene            1408..1770
                     /locus_tag="PLQ54_00845"
     CDS             1408..1770
                     /locus_tag="PLQ54_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91835.1"
                     /translation="MSTPPTAPPPPPPPGPGQYYTPPPSEGSGNRTLLIVLIVIGVLL
                     LVCIGGCITCGVLARQGFQQGMEEATRAGSADIKRLEAEVEKLPPGSPERAQKELELQ
                     QTRQALEVMRSMAGKRSP"
     gene            2077..2454
                     /locus_tag="PLQ54_00850"
     CDS             2077..2454
                     /locus_tag="PLQ54_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS91836.1"
                     /translation="MAKILVIDDDRAITRILQVVLGSGGHEVFVAQDGAHGLIAAHEI
                     HPDLILLDEMMPLLDGSSTLRALKASPETQPIPVVMVTVQNGNGYTRALYDLGVELHV
                     VKPFDPEEILEICNRLVPSECSS"
     gene            2691..3194
                     /locus_tag="PLQ54_00855"
     CDS             2691..3194
                     /locus_tag="PLQ54_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91837.1"
                     /translation="MAAHAPHLSLMAGNPGPPPPPRGQPKATASGPPRAETAKPAAPA
                     PRAVDPLSGRAPWDYEPVAKSLENVEEAELAPLSRFIEAMGAGWAIIPGVLWLLAALV
                     PIIIMKFGIEASGVVFWGSMVVPYLLVQVWAYLDIQDRGSPMWWLAVIFVVPVGIWLY
                     FMIERQE"
     gene            complement(3219..4322)
                     /locus_tag="PLQ54_00860"
     CDS             complement(3219..4322)
                     /locus_tag="PLQ54_00860"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOS91838.1"
                     /translation="MAWGTTVRTTLAGLPLALAASGAVSAGPSGGGNPLFFETRGLVA
                     FPSEIAALGLPQRAHDLGLNSIDAAYVDWLAKDAAGREFVNQCRRLDIDVVYAFHVVS
                     GCLPRSLFAEDPSMFRMNEKGERTPDANLCVHSERALEILCENAMEYVGTFYPASHRY
                     YLWGDDSRSWCHCPKCSQLSASDQALIMENRLLAALRQVDPRAEVSHLAYLATLEAPT
                     RVKPEPGVFLQFAPIERTTTKPLSDTDAFDPNYNLKHGEIVAALDANLDVFGRDGAEV
                     LEYWLDESRQARWDRTNLAPLRWNRESFLEDLRVYARAGIRRIRTYAAWIDEEYVTRF
                     GIPEALAEYAEGLRRWRWVNGSPIERRAPAARP"
     gene            4558..5043
                     /locus_tag="PLQ54_00865"
     CDS             4558..5043
                     /locus_tag="PLQ54_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS91839.1"
                     /translation="MSGWRGALTQADADGLFDQAFVEACGKLESQARGDGLVWRRRTP
                     GNTVPLLWRILERRAIDLIIRRREKRIAGALPPGWDEPSGEANPSDEVIDRAVRQRLR
                     DLILGETENRRAFLHLVLIEDWDEAEASRMLGVNENTGRSWYARFLKKCRGALRGWDA
                     P"
     gene            5040..6617
                     /locus_tag="PLQ54_00870"
     CDS             5040..6617
                     /locus_tag="PLQ54_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91840.1"
                     /translation="MTDASHRSNAQATGHVAPEVILLVLRGLADLDTAHAAYGHIRGC
                     EACAKRARELYQERIARRGLRSRLASVLHVSLPEVPRLRVAYAVAAVLLVLGLMVPIV
                     SGGGFLTRQDLDRKPFSAGIFGEAENAVARVYTATGAVDLAPEPEAFETPGMLHYPSP
                     PGPSAAIVLPSADPRHRDVLIGSLSPHRPGGRLHLVRWSQRTNRFDFLVPADVADFIP
                     QAILGGDQLIIRQLGVAESRDPNDLPRAIVWWRSREGDLSGLCVFAYDSRSDREPLRY
                     MATLTNVGWSFPVDQSKTKINGITRVPGLARDRLVVYWILSEATIGKDVVPVSGNGAG
                     IVVSLLDPFALKGASVTPFYALNPRVDAVWPVECGIGSWIIPRFLPDPGVGTKVTDTS
                     VQVQVPGCFDAFVNGRTYRVDAGAGTCSDAGALDFLKQNMRGAALGLADRLCPHQIYR
                     VEPQGTDRESLDLERQRLAALLKRDANEWSEAVRHLDPADLSPSYREHLYDMYVKPAA
                     QQRPGFFDRLTAPLRDR"
     gene            6729..>7498
                     /locus_tag="PLQ54_00875"
     CDS             6729..>7498
                     /locus_tag="PLQ54_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91841.1"
                     /translation="MIRLSGGRPLRRMGAPLLCLAIALCLCAAGENGCDGCGVVPEPG
                     PSNNDSNNSASPGPGSVRIRVTGPEGQPLVGVSATLSGQTLQTGSDGSALFRYLAATS
                     GALLALRHDGYVRNVKAVDVIADQEVAVDLAMAPVGVTQTIDASEQSSVRHREGGVTI
                     PANSLADAEGKLVRVAEATVTVALPSDERYTDVFPGEFVGTRENGGSVGLESFGIVDV
                     DLRDEAGNRLQLAQGKTADVIIPIDPAHDPGTPTVPLW"
BASE COUNT         1222 a         2533 c         2528 g         1215 t
ORIGIN      
        1 cgggagcgga cgcggcccat ggcgaggaac acacagccag ccgcacacat cgaccccgcc
       61 ggcgctccgc agcgtcaccc gactggtatg cacggcatcc gtcgaccact cgggcgccgg
      121 aacgagccga ggctcggggg gtgtctccgc cagcaccgcg agacgccgct cccagaatgc
      181 atcgatctcg gcggccgagg gcaacacgtc acaacctaga cgggtagcac gactggctga
      241 ccggttcggg ccgattgctt cactgccatg cacaactcga tggccttctt gccctcgctg
      301 ccgctcgtca gcggctcctt tccctcgcga acgcattcgg cgaagtggcg gaactcggcc
      361 gcgaagggat cgccgtcggt gaaggattcc gagtgcacct cagccggccg ggcgcggagc
      421 gcatagcgca ggtgggcggg attgtcgaag tcgcccgaga cctgcgcggt gccatgctcg
      481 caggcgatgt agaagtcctc gaaggccaga cccgggctgg gcgtgccgat catgccagcg
      541 ccgatcatcc cgatggcgcc gctggtggcg cgcaccgtgt agacggcaca gttgagctgg
      601 ggcgcgcgcc tgggggcgtt gaacacgtca ccctcggcat agagccgctc gatgtctccc
      661 agcaggaagc ggaccgtatc ggcggcgtga accgcattct cgagcagtgg cccgccgccg
      721 tcgtcctcgg cccagaacca ctcggcttcg gacatccacg gatgcggata gcgatggtag
      781 agcacctcgg gtcgccccag ttcggtgtcg atcagctcgc ggagtcgggc caccgcgggc
      841 acatagcgtt tcttgtgggc caccatgaga acgaccccac cggcggcgca ggcgtcgatc
      901 atcacctggc aggcttcggg cgtgggcgcc atgggcttct cgacgagcac gtgaatcccg
      961 cgccgcgcgg cgagttccgt gtgcggcaca tggaccttcg gcggcgtgca caggctgacg
     1021 gcatcgagcc gctctcgatc catcattgcc tcggcatcgc tgtaccgggc caccgccata
     1081 tcgcgggtgc gctcctccag caccgccggg ttgatgtcac acgcggccac aagctcgaac
     1141 tcgttcatcc cggcgaacac gcggagatga tgggccccga taccggccag tccaacgaca
     1201 ccaatgcgca tcagggtgac tcctgtcggg ttatgtggag tattctcttc ggctcgatcc
     1261 gccccagggc ctgccaggcg gccggcgctc gttcgtcgtc cctcgccttc gcccctggtg
     1321 ttcatgcgct ggccaactca ggcggaggat cagccgtcgc catcccgaat cctgagtgga
     1381 cggcccagcg catcgaggag gttcaccgtg tcgactccac ccactgcgcc ccctcctccc
     1441 ccgccacccg gtcccgggca gtactacacg ccgccaccct ccgagggcag cggtaatcgg
     1501 acgttgctca tcgtgctgat cgtcatcggg gtcctgctgc tggtatgcat cggcgggtgc
     1561 atcacttgtg gagtgctggc acgccagggc ttccagcagg gcatggaaga ggccacccgg
     1621 gccggttcgg ccgacatcaa gcgtctggag gccgaggtcg agaagctccc acccggctct
     1681 ccggagcggg cgcagaagga actcgaactt caacagacgc ggcaggcact cgaggtcatg
     1741 cggtcgatgg cggggaaacg gtcgccgtag ctggcgcagg ggcctcccta cgtacacgac
     1801 gtgttcgagg cccgcacgcg cggagcgggg ccacgcgcgc ttaggtctgt gggcgatggg
     1861 gtataagggg aacaaggcat cgtcgaatcg gcgggtggga gcatttgcgc cgcgaagggg
     1921 gtgactgccc acgtgatggc gaaaactccg gcggtcgagt tggtgcgagg atgtctgtgg
     1981 ctccgcgcgg actccggagt gggtttgtga tatcattgat tggtgttgtt tgctctttgg
     2041 cttgcaatcg ttgaagcacg cgcaaggcgg ggtatcatgg cgaagatcct ggtcatcgat
     2101 gacgatcggg ccatcacgcg gatactccag gtcgttctcg ggtctggagg ccacgaggtg
     2161 ttcgtggccc aggatggcgc acacgggctc atcgcggccc acgagatcca tccggatctg
     2221 atcctactcg atgagatgat gcctctgctg gacggctcga gtaccctccg cgcgctcaag
     2281 gccagtccgg agacacagcc gatcccagtg gtgatggtca cggtgcagaa cggcaacggc
     2341 tacacccggg cgctctacga cctgggcgtc gagctgcatg tggtgaagcc cttcgacccc
     2401 gaggagatcc tggagatctg caatcggctc gttccaagcg agtgcagttc ctagacctaa
     2461 cttgtgagcc atgggccggc gccatccagc tcgcgccggc ccgactgctt ctcccggtag
     2521 ggagagtgcg acaacccggc gaacagctcg gcggcgtgta gcgcctcacg ctcatctcct
     2581 tccggttcag gaggtgggga ggcatgtggc tcctccggca gtcccaccga ccgccatggc
     2641 ccgccagcac gccccctctc ggaatcctcc aacctgcatg ggcctgcccc gtggcagccc
     2701 acgcaccgca cctgtcgctg atggccggca acccagggcc accacctccg ccccgggggc
     2761 agccaaaagc cacagccagc ggccctcccc gggccgagac cgccaagccc gcagcccccg
     2821 cgcctcgcgc cgtcgaccca ctctcaggcc gggccccgtg ggactacgag cccgtcgcca
     2881 agtcactcga gaacgtggaa gaggccgaac tggcgcctct gagccggttc attgaggcga
     2941 tgggtgccgg ctgggccatc atccccggtg tgctgtggct gcttgcggcg ctcgtgccca
     3001 tcatcatcat gaagttcggc atcgaggcca gtggcgtggt gttctggggc tcgatggtcg
     3061 tgccgtacct cctcgtccag gtctgggcat acctggacat ccaggaccgc ggaagcccca
     3121 tgtggtggct tgccgtcatc ttcgtggtgc ccgtgggcat ctggctgtac ttcatgatcg
     3181 agcggcagga gtagtccggg gcacggctcg tggcgcactc aagggcgcgc tgccggggcg
     3241 cgcctctcga tggggctccc gttcacccac cgccagcgcc ggagaccctc agcgtactcg
     3301 gccaaggctt ccgggatccc gaaacgagtc acgtactcct cgtcgatcca ggccgcgtag
     3361 gtgcggatgc gccggatacc cgcccgggca tatacgcgca ggtcctcgag gaagctctca
     3421 cggttccacc gcaacggcgc caggttcgtg cggtcccacc gggcttgccg cgactcatcc
     3481 agccagtact cgagcacctc ggcaccgtcg cgcccgaaga catcgaggtt ggcatcgagc
     3541 gccgccacga tctcgccatg cttcaggttg tagttggggt cgaaggcatc ggtatccgac
     3601 agcggcttcg tagtggtgcg ttcgatgggc gcgaactgga ggaagacgcc cggctcgggc
     3661 ttcacccgag tgggggcttc cagcgtggcc agataggcca ggtgagacac ctcggctcgc
     3721 gggtcgacct gccgcagcgc cgcgagcagg cggttctcca tgatgagtgc ctggtcgctc
     3781 gcggacagtt ggctacactt cggacagtgg caccaggagc ggctgtcgtc accccagagg
     3841 tagtagcggt gagacgcggg gtagaatgtg cccacgtact ccatggcgtt ctcacagagg
     3901 atctcaaggg cccgttcgga gtgcacgcac agattggcgt cgggcgtgcg ctcgcccttc
     3961 tcgttcatgc ggaacatcga gggatcctcg gcgaacagcg accgcggcag gcagccggag
     4021 accacgtgga acgcgtagac cacgtcgatg tcgagccggc ggcactggtt gacgaactcg
     4081 cggcccgcgg cgtccttggc cagccagtcg acgtaggcgg catcgatgga gttgagccca
     4141 aggtcgtggg cccgctgcgg gaggcccaac gccgcgatct cgctcgggaa cgccaccagg
     4201 ccccgcgtct cgaagaacaa cgggttcccg ccgccgctag gcccggccga gaccgctccg
     4261 gatgcggcca aggcaagtgg cagccccgcg agagtcgtgc gtacagtcgt gccccacgcc
     4321 atgggtcgca accccctcac gccggccgag tcgaggccgg gaatggcgtg ttcgcggccg
     4381 cggcttcagc cgcctctcgg ccgcgcagga gagaagcccc actcatgcga aactgcgacc
     4441 atccgctggg ggagtgcaac ggaactgggc acgctagacg atactgtatg tgagagggaa
     4501 cgcgcgcgag tctttgcgct cctccagggc gctgagcgag ggctggtgcg ccgcctggtg
     4561 agcggttggc gcggggcgct gacccaagcc gacgccgacg gcctgttcga tcaagccttc
     4621 gtcgaggctt gtgggaagct cgagagccag gcgcgtggcg acggcctcgt gtggcgtagg
     4681 aggacgccag gaaacacagt acccctcctc tggcggatcc tcgaacgccg cgccatagac
     4741 cttatcatcc gcagaagaga gaagaggatc gccggggccc ttccgcccgg gtgggatgaa
     4801 ccatcgggtg aggctaaccc ttccgacgag gtcatcgacc gcgccgtgcg ccagcgcctg
     4861 agggacctga tactcggcga gaccgagaat cggcgggcgt tcctgcacct ggtgctcatc
     4921 gaggattggg acgaggccga ggccagcagg atgctgggcg tcaatgagaa caccgggcgt
     4981 tcgtggtatg cgcggttcct caagaagtgc cggggggcgt tgagggggtg ggacgcgccg
     5041 tgaccgacgc cagccaccgc tcgaatgctc aggccacagg gcacgtcgcc ccggaagtga
     5101 tcctgctggt cctccgcggc ctggccgatc tggacacggc ccacgccgcc tacggccaca
     5161 tccgcgggtg cgaggcgtgt gccaagcgcg cgcgcgagct ctatcaggaa cgcatcgccc
     5221 ggcgcgggct caggtcgcgg ctggcgtccg tcctccatgt ctcgctgccc gaggtgccac
     5281 gcttgcgcgt ggcgtacgcc gtggccgccg tgcttctcgt gctcggcctg atggtgccca
     5341 tcgtctcggg cggcgggttc ctcacgcggc aggatctgga ccgcaagccc ttctctgctg
     5401 gcatcttcgg cgaagccgag aacgccgtgg ccagggtcta cacggccacc ggcgccgtcg
     5461 atctggcgcc ggagcccgag gcgttcgaaa cgccaggcat gcttcactat cccagcccgc
     5521 ccgggccctc cgcggcgatc gtgctgccat cggccgatcc ccggcatcgt gacgtgctca
     5581 tcggctccct gtcgccccat cgcccgggtg gccggttgca cctggtccgc tggtcgcagc
     5641 gcaccaatcg gttcgacttc ctcgtgccgg ccgatgtggc cgacttcatc ccccaggcga
     5701 tcctcggggg tgaccagctc atcatccgcc agcttggcgt ggccgagtcg agggacccga
     5761 acgatctgcc tcgcgccatc gtgtggtggc ggtcgcgtga gggcgatctg tcggggctgt
     5821 gcgtcttcgc gtacgactcg agaagcgacc gcgagccgct gcgctacatg gcgaccctaa
     5881 ccaacgtggg ctggtcgttc ccagtggatc agagcaagac caagatcaac ggcatcaccc
     5941 gagtgcccgg gctggcgcgc gaccgcctgg tcgtctactg gatcctgagc gaggccacaa
     6001 tcgggaagga cgtggtgccg gtgtcgggca acggcgccgg catcgtcgtg agcctcctcg
     6061 accccttcgc gctgaagggc gcctccgtga ctcccttcta cgccctgaac cctcgtgtgg
     6121 acgcggtctg gccggtcgaa tgcggcatcg ggagctggat catcccgcgc ttcctccccg
     6181 atcccggggt cggcacgaag gtcaccgaca ccagcgtaca ggtccaggtg ccaggctgct
     6241 tcgatgcgtt cgtcaacgga cgcacgtacc gtgtcgatgc cggtgccggc acctgcagcg
     6301 acgccggggc gctcgacttc ctcaagcaga acatgcgcgg tgcggccctg ggactggcag
     6361 accgcctatg cccgcaccag atctatcggg tcgagcccca ggggaccgac cgcgagtccc
     6421 tcgacctcga gcgccagcgc ctggcggctc tgctgaagcg cgacgccaac gagtggtctg
     6481 aggccgttcg gcacctcgac ccggccgatc tctcccccag ctatcgcgag cacctttacg
     6541 acatgtacgt gaagccagcc gcccagcaga ggccggggtt cttcgaccgg ctcaccgcgc
     6601 ccctcagaga ccgctagcca actccccgtc accactccaa agattccgcg caaatgcgtg
     6661 caacgcttcg gtctcagcac gcgatagcac aagcggatgc cgccgatcgg cgtgaggagt
     6721 gttgcaaggt gattcggttg tccggcggtc gaccgctgcg ccgcatgggc gccccactgc
     6781 tgtgcctggc gatcgcgctc tgcctgtgtg cggcggggga gaacggctgc gacggctgtg
     6841 gggtagttcc ggagccaggg ccctcgaaca atgactccaa caacagcgcc agccccggac
     6901 cgggcagtgt gcggatccgg gtcacggggc ccgaaggaca gccgctcgta ggcgtgtcgg
     6961 ccacgctctc cggacagacg ctgcagaccg gcagcgatgg gtcagcgctg ttccggtatc
     7021 tggcggccac gtcaggcgcc ctgttggccc tgcggcacga cggctacgtg cgaaacgtca
     7081 aggccgtcga cgtcatcgcc gatcaggagg tggccgtgga ccttgcgatg gcccccgtcg
     7141 gcgtcacaca gaccatcgac gccagtgagc agtcgagcgt gcggcaccgc gagggcggtg
     7201 tgactatccc cgccaacagc ctggccgacg cagaaggtaa gctcgtgcgc gtggcagagg
     7261 cgaccgtaac cgtggcgctt ccgtcggacg agcgctacac cgatgtcttc ccgggggagt
     7321 tcgtcggcac gcgcgagaac ggcggcagtg tggggctcga gagctttggc atcgtcgatg
     7381 tcgacctaag ggacgaggcc ggcaaccggc tccaactcgc gcagggcaag acggcagacg
     7441 tgatcatccc catcgacccc gcacacgatc ctggcacgcc aaccgtgccg ctgtggta
//
LOCUS       DAOSPL010000024         7436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_9967_length_7436_cov_3.325837, whole genome shotgun sequence.
ACCESSION   DAOSPL010000024 DAOSPL010000000
VERSION     DAOSPL010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9967_length_7436_cov_3.325837"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PLQ54_00880"
     CDS             complement(<1..640)
                     /locus_tag="PLQ54_00880"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HOS91842.1"
                     /translation="MPLEGPSQVHRAPAPLLPVRVAGVTIAGRRVANEDAIYHEVRPP
                     RPGCVRAALAVADGESDEASGALASEIVLRKFRAGAAHAAQDPDIEPSRLLVHLCGRS
                     HEAIRAACEKQRATGNPRASFVGAYIVDNSLVLARAGNALAFLLRNGLIQAIGTQARD
                     AEDAPPADGARPVTEPEPEPALIGGDRVPDVSVFPGEDSVCPLRDGDVVILCS"
     gene            941..3286
                     /locus_tag="PLQ54_00885"
     CDS             941..3286
                     /locus_tag="PLQ54_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOS91843.1"
                     /translation="MPRWVMPALVMTVLHISGVDARVVHVAPGGDDAAAGTEAAPWAT
                     LTRAASSLAAGDTLVVHAGAYHGPMVIEASGTEAEPVVVQVEEGAEAVLEAPPGLGDA
                     SVGVLIRGKHVQLRGLTVRGFVGAGVQVGALVEPSAWDADHMRTDAYPERPADAADRV
                     AVSGVTVRDCGTGIVLGQSTHCSVRDCVVTGSGGPGLWLRALADHNTLIGNQMSGNQG
                     SGLCVDGGSSFNVIAENECSDNAGPGLLVTESCWRNVLGRNIAHGNGGPGLQCRGSRG
                     SAWVWNVVYRNRGAGLAIGEPNGRGSRNTSVVGNVFEANAGGGICVFAAANVAIHQNI
                     ATANAHCQLFVSDWAIETGLHSIDRNCLHAPEGPAIAWGGLPEDWQPPAATVEATGLP
                     ALTGVASDNIGADPKFVAPLPECTLAEGSPCLPEEEGQERIGPNMVTPLPDDIPSPPP
                     DDLPAATRSGTEVLRACLACSDPWVVERALTVARRAATSPSAYVDLEALVPDLQAVAA
                     GPGVSPQTQWGALIVRMAAADALAHPEVEAMAAGAGAWELVRAADAWLAGGDMVALEA
                     LRPRAEEFDPWFQASLAARLAEADGEGAADSLSMLLESGSLLSRVIVASALARLHDPA
                     GAEALRAELARASGPAVVMVLQGIRESRDSQLAADVAQALAATTDADALAAALETLGA
                     LADVSTVQAVATHAEDEEPMVRMAAIEALLRLGQPADVTAVRAALRDPQGSRRAAAAR
                     LAGELGEAEDVATVEELMSGDPNPIVRLGALEGLIRRAARE"
     gene            complement(3417..5039)
                     /locus_tag="PLQ54_00890"
     CDS             complement(3417..5039)
                     /locus_tag="PLQ54_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91844.1"
                     /translation="MGARFTIRGETRRAFLARVAGATAALWMSGGESVSGMGAAHGRR
                     FHISASAHAIETYPDLPEVARAAGITDVWLAGFLYGTWYRRPDALRELVAIWERQGIR
                     THAINVPLGHPGNALGVSSDSVPTTPPGSWRMARGVDGNWFSGTSVHDPAPAENARAA
                     RELQDAGFRELFLDDDFRIAQSPGTIGGCFCDDCRDEFLRLTGYPASAFEDLRQTIRD
                     RRPTPMLRQWIEFWCDKLWAMIRGMQIAAPRMAIGPMVMYLGSEKAGIALDRYRRVPF
                     RVGELMFSDAEFAPVKGKTDELFSCLFHRRFASPELAYSETTAYPEDQLSAANMAAKL
                     TISLIGDVRNTMYMSGLMPFPIEHWAVLGPAMKESARLHDLVAGHPPRGPFKHYWGWD
                     SRLVGNDQPFSLFLATGVPFEVVDDLPDDGWAFLSDADARAVAEGRLKPRCRHLVARP
                     HAATAGPPLTAVPEDLAALFALKARIAPSLRGVPYVEGEVPVVFAWYPTARRGIIWNL
                     TEASQEFRVVRDGRVVFETRLGPLGVAATARM"
     gene            5136..6500
                     /locus_tag="PLQ54_00895"
     CDS             5136..6500
                     /locus_tag="PLQ54_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOS91845.1"
                     /translation="MPRAVALLSEALVICGSAATVAQDEPDASPWERVRQRYEQLRTE
                     LSKSRRPYGVWLRDANELWVYDNRTELGIGMHDTSDAQLDLLPPLGIRLVRKTLYWNR
                     MENTTQHGQYDAAYLAEWDSLAKRAEKRGIDLLVVVHGNAPGTGWANREASYERFARF
                     MADMATRYPTIRLWELWNEMDSGFTDLFGAGVEPAVPMLERGRHYAEMLKVAYRAIKQ
                     ANPDAWVLTGGMTDWQEFPRGIYEGGGRDAFDFMNVHTYGVPVVWSLVTRGAELRQVM
                     AEFGDAERPLWNTEFGIDAGALVAAWGLPHERGEDDGPAFDRLQLEQWKECIEAGFET
                     GLYAKLLPYQLLAGNEGQNDRLKTQAYAEEYLPAGHTIDDYGFGIVRSDGKTPRPTYE
                     WLAAKRWNDPAAEAPHVTVDVEVSPAPEGDPVDYGFERRADGAMVIRDVRLDRLFPTR
                     IRLE"
     gene            complement(6790..>7436)
                     /locus_tag="PLQ54_00900"
     CDS             complement(6790..>7436)
                     /locus_tag="PLQ54_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOS91846.1"
                     /translation="CVEDASFVRTREPDDDFHWGRVRPLRDLGWAALGWIAIANDARM
                     VHAIRSQLSQDVRHSAPVDALNLISEGYVTIPAALAATLFGEGELHDTGKLALSGILL
                     TAATVDSLKTLTGRARPDGAGGVGSFGGPFAGGDSFPSGQTAAMFSLATVIGKRHPKA
                     KWWAYALATGTAISRVFLDRHHPSDVLFGALVGIETGNFVLRNDGWLTRISIKF"
BASE COUNT         1178 a         2457 c         2709 g         1092 t
ORIGIN      
        1 cgctgcagag gatgacgacg tcgccatcgc gcagaggaca cacactgtcc tcgccgggga
       61 acacgctgac gtccggtacc cgatcgccgc cgatcagggc gggttcgggc tcgggttcgg
      121 tgacggggcg agcaccgtcc gctggaggcg cgtcctcggc gtcgcgggct tgggtcccga
      181 tggcctggat caggccatta cgaagcagga aggcgagggc attgcccgcg cgggcgagga
      241 cgagcgagtt gtcgacgatg taggcgccga caaagctggc ccgcggattg cccgtggccc
      301 gttgcttctc gcacgccgcg cgaatggcct cgtgcgaccg gccgcagagg tggaccagaa
      361 gccgggaggg ctcgatgtcg gggtcctggg ccgcatgggc ggcaccggcg cggaacttgc
      421 ggagcacgat ctcgctggcg agggcccccg aggcctcgtc gctctccccg tccgcgacgg
      481 ccagcgccgc gcgcacacag ccgggccgcg gcgggcggac ctcgtggtag atcgcgtcct
      541 cgttggccac ccggcggccg gcaatggtga ctccggccac gcgaacgggc agcagcggcg
      601 ccggagcccg atggacctgg ctcgggcctt ccagaggcat gggcggctcc cccggtatca
      661 cttcggcatc aaaccgacgc gtgtttcttt gagcgaccat cgggaaacac cttttcggag
      721 atctggaagc gctcgaagta tctggctcgc gcccgtcggg gcgggccact cctcgggcag
      781 gcgatatcct cgtcagactc gaaaccaccg gggagagaga tcggttcggc gaccacacag
      841 gaccgggacc gggtggaacc tgcgggggcg ccgggcgtct ctcctacggt atggccggtc
      901 gccggctcga ggttcagctg tgccaagggg aggggtttgc atgccgagat gggtgatgcc
      961 cgcactggtg atgaccgtgc tgcacatctc cggcgtcgac gcgcgcgtgg tgcacgtcgc
     1021 tcccggcggc gatgatgcgg ctgccggcac cgaggcggcc ccttgggcga cactcacccg
     1081 tgcggcatca tctctggcgg cgggggacac gctggtggtt cacgccgggg cctaccacgg
     1141 gcccatggtg atcgaggcct caggtaccga agccgagccc gtcgttgtgc aggttgagga
     1201 gggggccgaa gcggttctcg aggctccgcc cggcctgggc gacgcctcgg tgggggttct
     1261 catccggggc aaacacgtgc agctccgcgg actgacggtc cgcggtttcg tcggcgcggg
     1321 ggtccaggtg ggcgcactgg tggaaccctc ggcttgggac gcggaccaca tgcggaccga
     1381 tgcctacccc gagcggcccg ctgacgccgc cgaccgggtg gccgtcagcg gcgtcaccgt
     1441 ccgcgactgt ggcacgggca tcgttctggg ccagtcgacg cactgcagcg tgcgcgactg
     1501 cgtggtcacc gggagcggag ggccgggcct ctggctccga gcgttggccg atcacaacac
     1561 cctgatcggg aaccagatgt ctggcaacca gggctccggg ctatgtgtcg atggcgggtc
     1621 atcgttcaat gtcatcgccg agaacgagtg ctccgacaac gcgggtccgg gcctgctggt
     1681 caccgagagc tgctggcgga acgtgctcgg ccggaatatc gcgcatggca acggcggccc
     1741 gggcctgcag tgccgcggct cgcgcgggtc ggcctgggtc tggaatgtgg tgtaccggaa
     1801 taggggcgct gggcttgcca tcggcgagcc caacgggcgc gggtcgcgga acacctcggt
     1861 cgtggggaat gtgttcgagg ccaacgccgg ggggggaatc tgcgtgttcg ccgccgccaa
     1921 cgtggcgatc caccagaaca tcgccacggc caacgcccac tgccagctct tcgtctccga
     1981 ttgggcgatc gagaccggat tgcactcgat cgaccgcaac tgcctccatg cgcccgaagg
     2041 gcccgccatc gcgtggggcg gccttccaga ggactggcag ccaccggccg ccaccgtgga
     2101 agcgaccggc ctacccgctc tgacgggcgt ggcgtccgac aacatcggcg ccgaccccaa
     2161 gttcgtggcg cccttgcctg agtgcactct ggccgaggga agcccctgtc tgcctgagga
     2221 ggaagggcag gaacggatcg gccccaacat ggtgacgccg ctgcccgacg atatcccgtc
     2281 gccacctccc gatgatcttc cggctgccac tcgctcgggc accgaggtgc tccgggcgtg
     2341 tcttgcctgc agcgatccct gggtcgtcga gcgggccctc accgtggcgc gccgcgcggc
     2401 cacgagtccc tcggcgtatg tcgacctcga agcgctcgtg cccgacctgc aggccgtggc
     2461 cgcagggccc ggcgtctcgc cgcagaccca gtggggtgcg ctgatcgtga gaatggcggc
     2521 ggcagacgct ctggcccatc ccgaggtcga ggcgatggcc gccggagccg gtgcgtggga
     2581 gctggttcgc gccgccgatg cttggttggc cgggggcgac atggtcgcgc tcgaggccct
     2641 gcggccgcgg gctgaggagt tcgacccatg gttccaggca tcgctcgcgg ctcgcctggc
     2701 tgaggccgat ggcgaaggtg ccgccgactc cctgtcgatg ctgctcgaga gcggcagcct
     2761 gctgagtcgg gtcatcgtcg cgtcggcgct ggcgcggcta catgatccgg cgggcgcgga
     2821 agcgctgagg gccgagctgg cccgggcatc gggtccagca gtcgtcatgg tactccaggg
     2881 gatccgcgag tcgcgcgaca gccagctggc ggccgatgtg gcccaggccc tggcagcgac
     2941 cacggatgcg gatgcactgg ccgcggcgct tgagacactg ggcgcgctgg ccgacgtgtc
     3001 cactgtccaa gccgtcgcga cgcatgccga ggacgaggaa ccgatggtca ggatggcggc
     3061 aatcgaggcg ttactgcgcc tggggcagcc agcagacgtg acggccgtgc gcgcggcctt
     3121 gcgcgacccg cagggctccc ggagggccgc cgcggcccgg ttggccggag agctgggcga
     3181 ggccgaagac gtggccacgg tcgaggagct catgtcgggc gacccgaatc ccatcgtccg
     3241 cctgggggcc ctggaaggcc tgatccggcg ggcggcgcgg gagtaggggc aggcaggcca
     3301 cggcccgcgc gggctccacg gggcggccgc actgggcaac gaccgcgaac ccggcggctc
     3361 ctcatccgtg tacggcgccg cccgtgcgtg cggggacacc agctcctacc catcgatcac
     3421 atcctggccg tcgctgcgac cccgagcggc cccaggcggg tctcgaacac gactcgaccg
     3481 tcacgtacga cgcggaactc ctgtgaggct tcggtgagat tccagatgat gccgcggcga
     3541 gccgtgggat accacgcgaa caccacgggc acctccccct cgacgtacgg cacgccgcgc
     3601 agcgagggcg cgatgcgcgc cttgagggcg aacagggccg ccaggtcctc cggcacggcg
     3661 gtcagcggcg gcccagctgt ggccgcgtga ggccgtgcga cgaggtgtcg gcatcgcggc
     3721 ttgagccgac cctcggccac ggcccgcgca tcggcatcgg agagaaatgc ccagccgtcg
     3781 tcgggcaggt cgtccacgac ctcgaagggc acgccggtgg cgaggaacag gctgaagggc
     3841 tggtcgttgc ccacgagccg gctgtcccaa ccccagtagt gcttgaacgg cccgcgcggc
     3901 ggatggccgg cgaccaggtc gtgcagccgc gcgctctcct tcatggccgg gccgagcacc
     3961 gcccagtgct cgatcgggaa gggcatgagc ccgctcatgt acatggtgtt gcggacatcg
     4021 ccgatgagcg agatggtgag cttggcggcc atgttcgcgg ccgagagctg atcctccggg
     4081 taggcggtgg tctcgctgta ggccaactcg gggctggcga agcgccggtg gaacaggcaa
     4141 ctgaacagct cgtcggtctt cccctttacc ggtgcgaact cggcatcgga gaacattagc
     4201 tcaccgacgc ggaacgggac gcggcggtag cggtccaggg cgatgccggc cttctccgag
     4261 ccgaggtaca tgaccatggg accgatcgcc attcgcggcg cggcgatctg catgcctcgg
     4321 atcatggccc agagcttgtc gcaccagaac tcgatccact ggcgaagcat cggggtgggg
     4381 cgcctgtcgc ggatcgtctg ccggaggtct tcgaacgccg aagccgggta accggtgagg
     4441 cggaggaact cgtcgcgaca gtcgtcgcag aaacatccgc cgatcgtgcc cggcgactgg
     4501 gcgatgcgga agtcgtcatc gaggaacagc tcgcggaacc cggcatcctg cagctcgcgc
     4561 gccgcccgag cgttctcggc cggtgctgga tcgtggaccg aggtgcctga gaaccagtta
     4621 ccatcgacgc cgcgagccat ccgccaactg cccggcggcg tggtggggac gctgtcgctc
     4681 gagacgccca gggcgttgcc ggggtggccc aggggcacgt tgatggcgtg ggtgcggatg
     4741 ccctgcctct cccagatggc gacgagctcg cgaagggcgt ccggccggcg gtaccaggtg
     4801 ccgtagagga agccggcgag ccagacatcg gtgataccgg ccgcacgagc cacctccggc
     4861 aggtcggggt aggtctcgat cgcgtgagcg gaagccgaga tgtggaaacg ccggccatgg
     4921 gccgctccca tgcctgacac cgactcacca cccgacatcc acagagccgc tgtggccccc
     4981 gccacccggg ccaagaacgc gcgccgggtc tctccgcgaa tcgtgaaccg ggcacccact
     5041 gctctacacc ccttcagctc gcccactcgc cgaccggacg ggtggtgggt tcgcgcgcca
     5101 tgctagactg tcctcgaatg acacggaggt ggcccatgcc ccgagccgtt gcactgctgt
     5161 ctgaagcgct cgtgatatgc ggctccgcag cgacggtggc gcaggacgaa cccgacgcga
     5221 gcccctggga gcgcgttcgg cagcgctatg agcagctccg tacggagttg agcaagagcc
     5281 gccggcccta tggcgtgtgg cttcgcgacg ccaacgaact gtgggtctat gataaccgca
     5341 ccgagcttgg catcggcatg cacgacacat cagacgctca gctcgatctg ctgccgccgc
     5401 tgggcatccg gctcgtccgc aagacactgt actggaatcg gatggagaac acgacccagc
     5461 acggccagta cgacgccgcc tacctggccg agtgggacag cctcgcgaag cgcgcagaga
     5521 agcgcggcat cgacctgctg gtcgtcgtcc acgggaacgc gccgggaact ggctgggcca
     5581 accgcgaagc cagctacgaa cgcttcgcgc gcttcatggc cgacatggcc acccgctacc
     5641 ccaccatccg gctctgggag ctctggaacg agatggactc gggcttcacg gatctgttcg
     5701 gggccggggt cgagcccgcc gtgcccatgc tcgagcgtgg taggcactac gccgagatgc
     5761 tcaaagtggc ctatcgggcc atcaagcagg cgaacccgga cgcgtgggtg ctcaccggcg
     5821 gcatgacgga ctggcaggag ttcccgcgcg gcatctacga gggcggcggg cgagacgctt
     5881 tcgacttcat gaacgtccac acgtacggcg tgccggtggt gtggagcctg gtgacgcgcg
     5941 gggccgagct gcgtcaggtg atggccgagt tcggcgatgc cgagcggccg ctgtggaaca
     6001 ccgagttcgg catcgacgcc ggggcactgg tcgccgcgtg ggggctgccg catgagcgcg
     6061 gcgaggacga cggtccggcc ttcgaccgcc tgcagctcga gcagtggaag gaatgcatcg
     6121 aggccggctt cgagaccggc ctctacgcca aactgctgcc ctaccagctc ttggcgggga
     6181 acgaagggca gaacgaccgg ctgaagacac aagcctacgc cgaggagtat ctgcccgccg
     6241 gacacacgat tgacgactac ggctttggca tcgtgcggag tgatgggaag acaccgcggc
     6301 caacctatga gtggttggcg gccaagcggt ggaatgatcc agcggccgag gcgccgcacg
     6361 ttacggtgga cgtcgaggtc agcccggcgc cggagggcga cccggtggac tacggctttg
     6421 agcggcgcgc ggatggggcc atggtcatcc gcgacgtgcg actcgaccgc ctgttcccga
     6481 cgaggatccg gctggagtag cggcggtaga acggcgcgag gcgcaagagc gggtggggac
     6541 tgagccctgc ggcacagcct ccaagagcga ccgtaaggcg tcgcgtaggg ctctgtccca
     6601 ttggttgccc gactcggcgc cgccggttag cccgccgatt gccgcagggg ggacagagcc
     6661 cgttgccgat ggagccggcg cttgtccgcg agctggtgtg gcaacgtgct cagtccccgg
     6721 tgccgttcct tggcgtcggc cggcgaagga acaggtaggg gtcggtctgt acccgcaagc
     6781 actgcgcggt cagaacttga tcgatatccg cgtgagccat ccatcgttgc ggagcacgaa
     6841 gttccccgtc tcgatgccga cgagggcacc gaagagcacg tcggacggat ggtggcggtc
     6901 caggaagacc cgactgatcg cggtccccgt tgccagtgca taggcccacc acttggcctt
     6961 ggggtgccgc ttgccgatga ccgtcgcgag ggagaacatg gccgccgtct ggccggacgg
     7021 gaacgagtcg ccgcctgcga atggcccccc aaatgatccc acgccgcccg cgccgtcggg
     7081 tcgcgcgcgc cccgtcaggg tcttgagcga gtcgacggtg gccgcggtga gcagaatgcc
     7141 ggacagggcc agcttgccgg tatcgtgcag ctcgccctcg ccgaaaagcg ttgcggccag
     7201 ggcggcaggg atggtgacat agccctcgct gatgaggttg agcgcgtcaa ccggagccga
     7261 gtgccgcaca tcctgggaca actgagagcg aatggcgtgc accatgcgcg catcgttggc
     7321 aatggcgatc cagccgagag cggcccagcc caggtcccgc aggggccgca cccggcccca
     7381 gtggaagtcg tcgtccggtt cgcgtgtccg cacgaacgat gcgtcctcaa cacata
//
LOCUS       DAOSPL010000025         7277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_10269_length_7277_cov_3.491138, whole genome shotgun sequence.
ACCESSION   DAOSPL010000025 DAOSPL010000000
VERSION     DAOSPL010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7277
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10269_length_7277_cov_3.491138"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2043)
                     /locus_tag="PLQ54_00905"
     CDS             complement(<1..2043)
                     /locus_tag="PLQ54_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91847.1"
                     /translation="MTAVGDHAQGDSRHVGEPALRFVAWAAACVTLWLLFMAPASGQV
                     PSPPTSVAAADTPADQGGAISLSWTESVSKPVDEYRIYRRTAPTGLPVLRGTAPDGAT
                     TALDASTDGIDYYYTVRAVVGGVESADSNMAGPVRSLDNLAPSPPTSVTAVDRPADNG
                     HALVVSWELSVDDGGGVKDVKTYRIYRSVAPGPAGVLVGTVAAGIQSYIDSSTDIANG
                     ITYYYRVRAFDGVNESVNSATEGSAVAVDNVPPGAPRSPAVADAPGDSGGQIDVTWAL
                     SIDDGAGARDVIEYRIYRTTTPGVYLDPAIGTSSAGSTTFRDTTAVGHDYYYVIRAFD
                     GTAESASSPERGPVTAVDNRPIFPPNTVVVTDVPGDQGTALTLSWARSADAPGAGGRD
                     LVTEYRVYRRLSGTSVFGLVATLPVTTLTYTDNAVVKTKQYDYTVRASDGLAESPDAN
                     VVGPISPIDDIPPATPTNIAAADTPLDNGKSISVTWLPSDDDRTGGDVTEYRIYRSTV
                     PSGFAPPAVGTVPKGSTLFVDTTAPNGVPEYYMVRAYDGSLESSDSSVASATAEDNIK
                     PGAVSNVKAIDTPSDTGGSITITWTASPDDRVDVVEYRIRRGTTIDSQPDVAGRVPAG
                     VTTFVDDGLPNQTPFYYVIEAWDGTFLSDPSRVTGPVSAVDNKDLLAPTAVLARDHP"
     gene            complement(2235..3302)
                     /locus_tag="PLQ54_00910"
     CDS             complement(2235..3302)
                     /locus_tag="PLQ54_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS91848.1"
                     /translation="MSTGRVRVLVADDEEPLVSLLCTMLNDLGYETSPVYGGLEALSA
                     LANGRYDVVITDLVMPDVDGLTVLRRCKECPGPVEAIVLTGQDDVETAVACMKAGAYD
                     FLTKPFRLDNVASVVGKAAAKVQADEENRHLRDTLYASQRTLMNALDAKDTYTCSHCV
                     NVARLALSFCDHLGLSPEVRDAIRKAGELHDVGKIGIADHILNKPGRLTGPEYEEMKR
                     HPVIGKRIVDPLGVFGVEGDLLYYHHERWDGRGYPDGLSGDTIPLVARVLAVVDVFDA
                     LTSDRPYRAALALDEALRLIEAGIGSQFDPAVGRAFLEFLEAEPELIAPPAALADEPG
                     SYADEPMAVSAGPAAGSSLQD"
     gene            complement(3481..5421)
                     /locus_tag="PLQ54_00915"
     CDS             complement(3481..5421)
                     /locus_tag="PLQ54_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOS91849.1"
                     /translation="MVLVRLQNVAKCFGANEVLRDVTWQIDRGQKIGVVGENGVGKST
                     LFRIITGACTPDRGTVTLHRGIRIGLLAQSPELDLERSAHEEMLLCRPDLRECERHIE
                     ELAALISEPELDPKNRSRDRLLTLHSEAIESYQRAGGMEFEARIGRTLAGLGLPADHH
                     TVPLGHLSGGQRSRVALAKVLVDETDLLLLDEPTNHLDIEAMEWLQEFLRSYDGGFVT
                     ISHDRYFLDAVAERIAEVEDGVLSEHAGGYTAYAEAKDRELQAQAKAYEVQQRYLRRQ
                     EEFIRRMMQRKTEKAVRVGQSRARMIAKLERVERPTLQRRRPLLQFAQPTSGTNDILQ
                     CAGVAKRFGERILFHGLDLILNRGNRMGIVGPNGTGKTTLLRMVLGEETASEGTLRLA
                     PTVRVGYYAQHREELDPALTIMDHVGHARPDLSKQEIRDYLARFLFFGDDVFLPVEAL
                     SGGERSRVALALLILSRPDFLLLDEPTNHLDTTSREVFEDALVDYTGTILVASHDRYF
                     LDRTVDRLLILGESPPEVFLGNYSALQAWREAEHRRQQEAEMARRAEARRVARQRARA
                     APRAAAKPQPAGPGPEEIERAISETEAHISEVSQLLGSPQVYADGERVRRLSLEYEDL
                     TARLNTLYARYLEVAEARAAKD"
     gene            5557..7050
                     /locus_tag="PLQ54_00920"
     CDS             5557..7050
                     /locus_tag="PLQ54_00920"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOS91850.1"
                     /translation="MPLLEMRQISKSYHGVPVLREVDLEAEGGEVHALVGENGAGKST
                     LMKILDGAVRADQGSIRLGGELVDIPSPRAAQQLGVDVIHQELLLAPSLDAAENIFLG
                     RTPSRGRVFVDRRTLYQRAEAVLAELGADLDVRVPLRRLTVAQRQMVAIARALSAEAR
                     IVVMDEPSAVLTRNELERLFEAIGRLCARGVAVVYISHRLDEVFRLAARVTVLKDGQS
                     QGTYPIGELTRAKVIRLMVGRDIEVTVSRTDQSRPVEVLRVEGMRSGRRVRGCDLSLR
                     QGEILGIAGLVGAGRTELARAIVGADRRDAGQVYVDGRSVHIGSPAEAIRHGIAYIAE
                     DRKTCGIFPELGVADNVLLPTYENASRWGVVGLGSARRTVRALVERLDVRAADLAQRV
                     VTLSGGNQQKVVLARWLNRQARIFIFDEPTRGIDVLAKEHIYGLMNQLIDGGASIVMI
                     SSELPELLAMSDRILAMREGLIVGEISRAEATEERVLAMCMGQMQVA"
     gene            complement(7084..>7277)
                     /locus_tag="PLQ54_00925"
     CDS             complement(7084..>7277)
                     /locus_tag="PLQ54_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HOS91851.1"
                     /translation="AGGLGNIVAGVVSRHPASAGALIDQIGVCGFGESGDPWVLMKAF
                     GLCAEFIAQRGLKVLKEKG"
BASE COUNT         1165 a         2316 c         2527 g         1269 t
ORIGIN      
        1 cggatggtcc ctcgcgagga cggcggtggg ggccagcagg tccttgttgt cgacggccga
       61 gacgggaccg gtgacccggg agggatccga caggaacgtc ccgtcccagg cctcgatgac
      121 atagtagaag ggcgtctggt tcggcagtcc gtcgtcgaca aacgtggtga cgcccgccgg
      181 cacacggccc gcaacatccg gctggctgtc gatcgttgtg ccccggcgaa tgcggtattc
      241 gacgacgtcg acacggtcgt cgggcgatgc ggtccaggtt atggtgatcg aaccgccagt
      301 gtctgagggt gtatcgatgg ccttcacatt ggagacggca ccgggcttga tgttgtcctc
      361 ggcggtggcg gaggcaaccg aggagtcgga tgactccagg ctcccgtcgt acgcgcgcac
      421 catgtagtac tcgggcacgc cattgggggc tgtggtgtcg acgaacaagg ttgagccctt
      481 gggcacggtt cccacggccg ggggggcgaa gccgctgggc actgtcgacc ggtagatacg
      541 gtactcggtc acatccccgc cggtcctgtc gtcgtcggag gggagccatg tcacgctgat
      601 gctcttgccg ttgtccaagg gggtgtcggc cgcggcgatg ttcgtgggag tggccggcgg
      661 gatgtcgtcg atgggactga tcggccccac cacgttggca tcgggggatt ccgcaagtcc
      721 atcggaggcc cgcacggtgt agtcatactg cttcgtcttc actacggcat tgtcggtgta
      781 ggtcagagtc gtcacgggga gtgtcgcgac cagaccaaag actgaggtgc cgctcaggcg
      841 acggtatacc cggtattcgg taaccaggtc gcggcccccg gcccccgggg cgtcggccga
      901 gcgggcccaa ctcaacgtca gcgcggtgcc ctgatcgccc gggacgtcag tcaccaccac
      961 tgtgttgggg gggaagatgg ggcggttgtc caccgccgtc accggtcccc gttcggggct
     1021 cgaggccgac tcggctgtcc cgtcgaaggc ccggatgacg tagtagtagt cgtggcccac
     1081 ggccgtggtg tccctgaacg tggtgctgcc ggccgacgag gtgccaatgg cggggtcgag
     1141 gtacacaccg ggcgttgtcg ttcggtagat gcggtactcg atcacgtccc tggcaccggc
     1201 gccgtcatcg atggacaagg cccaggtcac atcgatctgg ccaccggagt cgccgggcgc
     1261 atcggccacc gccggtgacc tcggggcgcc gggaggcacg ttatccacgg ccacggcgct
     1321 gccctcggta gcggagttga ccgactcgtt gacgccgtcg aaagcccgaa cccgatagta
     1381 ataagtgatc ccgttggcaa tgtcagtgct cgagtcaatg tagctctgga tgccagcggc
     1441 gacggtgccc accaggacgc ctgccggtcc cggagccacc gaccggtaga tgcgataggt
     1501 cttgacatcc ttgacgcccc cgccgtcgtc cacagatagc tcccatgaca cgacgagggc
     1561 gtggccgttg tcggccggcc ggtccaccgc ggtgacgctg gttgggggcg acggggccag
     1621 gttgtccaac gaccgcacgg gcccggccat gttggagtcg gccgactcga cgccgccgac
     1681 cacagcccgg accgtgtagt agtaatcgat gccatcggtc gatgcgtcca acgcggtggt
     1741 ggcaccgtcc ggtgcggtcc cgcgtagcac ggggagccct gtgggagcgg tccggcggta
     1801 gatgcggtat tcgtccaccg gtttgctgac ggactccgtc cagctgaggg agatggcgcc
     1861 gccctggtcg gccggtgtgt cggcggccgc cacggatgtg ggcggcgagg gcacctggcc
     1921 gctggccgga gccatgaaca aaagccacag cgtcacacac gcggcagccc aggccacgaa
     1981 ccggagagcc ggctcgccca cgtgccgcga gtcgccctgt gcatggtccc ctaccgcagt
     2041 cacggcctgt ctcccagccg cccccgtctc agcgggtgcg ggcagcgtct cgtgcctcga
     2101 cccgagtctg aacggggctc acccggtcat tcggacgtgc cccaagtccg ctcggactcg
     2161 gcaccaccac gcaagagagt tgtgccacag tgggggcttc ccctttgggg tatcggctgg
     2221 aatcgcctcg gtccttagtc ctggagcgaa cttccggctg cggggccagc ggagaccgcc
     2281 atgggctcgt cggcgtagct gccgggctcg tcggcaaggg ccgcgggggg tgcgatcagc
     2341 tcaggttcgg cctccaggaa ctcgaggaac gcccgaccga ccgccgggtc gaactgagac
     2401 ccgatcccgg cttcgatgag gcgcagggcc tcgtccaggg ccagggccgc tcggtagggc
     2461 cggtcactgg ttaatgcgtc gaagacatcc accacggcga gcacccgggc cacgagggga
     2521 atggtgtcgc ccgacagccc gtcaggatag cctcggccat cccagcgttc gtggtggtaa
     2581 tacagcaagt cgccctcgac tccgaacacg cccagcgggt cgacgatccg cttgccgatc
     2641 accggatggc gcttcatctc ctcgtattcc ggcccggtga gccgacccgg cttgttgagg
     2701 atatggtcgg cgatgccgat cttgccgacg tcgtgtagct ctcccgcctt gcggatggcg
     2761 tcgcgcacct cgggcgagag gcccaggtga tcgcagaacg aaagcgccag ccgcgccacg
     2821 ttcacgcagt gactacatgt gtacgtgtcc ttggcgtcca gcgcgttcat gagtgtccgc
     2881 tgactggcat agagcgtatc gcggaggtgc cggttctcct cgtcggcttg gactttggcg
     2941 gctgccttgc cgacgaccga ggccacattg tcgagacgga agggtttggt taggaagtca
     3001 tatgctcccg ctttcatgca ggcaacagcg gtctccacat cgtcttggcc ggtgagaacg
     3061 atggcttcga cggggccggg acactccttg cagcggcgca gcacggtgag tccatcgacg
     3121 tcgggcatga ccaggtcggt gatcacgaca tcatagcggc cattggccaa cgccgagagc
     3181 gcttcgagtc caccataaac tgggctggtc tcatagccca gatcgttcag catggtgcag
     3241 agcagagaga ccaacggttc ctcgtcatcg gccaccagca cccgtacgcg gccggtgctc
     3301 atctcgcacc tcctcgccgc ttcccccaac gtatccgccg tgcggcactc ccagctgcac
     3361 agtacgcctt gccgattcta gccttccctg cctcccggtg cacgagtggc accgaagttt
     3421 gaagtgtacg agaccagccg taaggcgaag cgtgccctca ttggtactcg acgggccacc
     3481 tcagtccttc gccgcgcgcg cctcggcgac ctccaggtag cgcgcgtaga gggtgttgag
     3541 acgagccgtg aggtcctcat actccaacga gagacggcgc actctctcgc catcggcata
     3601 cacctgggga gagcccagga gttgggagac ctcggagata tgggcctcag tctccgagat
     3661 ggcccgctcg atctcttcgg gtcctgggcc agccggctga ggcttggcgg cagcccgggg
     3721 cgcggcccga gctcgctgcc tggcgacgcg tcgcgcttcg gcgcgccgcg ccatctcagc
     3781 ctcttgctgg cgccggtgtt ccgcctcgcg ccaggcctgg agtgccgagt agttgcccag
     3841 gaacacctcg ggcggggact cgccgaggat cagcagccgg tcaacggtgc ggtccaggaa
     3901 gtaccgatcg tgagatgcca cgagaatggt gcctgtgtag tcgacgagcg cgtcctcgaa
     3961 gacttcacgg gatgtggtgt cgaggtggtt cgtgggctcg tcgagcaaca ggaagtcggg
     4021 ccgcgagagg atgaggagcg ccagggcgac ccggctacgt tcgccccctg atagcgcctc
     4081 cacgggcagg aacacatcat cgccgaagaa caggaagcga gccaggtagt cccggatctc
     4141 ctgtttggag aggtccggcc gggcatgccc gacgtggtcc atgatggtca gggcgggatc
     4201 gagctcttcg cggtgctggg cgtagtagcc cacccggacc gtgggcgcga ggcgcagcgt
     4261 accctcggag gcggtttcct cgcccaacac catgcggagg agagtcgtct tcccggtgcc
     4321 gttggggccg acgatcccca tgcggttgcc ccggttcagt atcagatcca ggccatggaa
     4381 gaggatcctc tcgccaaacc gcttggcgac accggcacac tggaggatgt cattcgtgcc
     4441 tgacgtgggc tgggcgaact gcagcagggg acggcgccgc tggagggttg gccgctcgac
     4501 cctctcgagc ttggcgatca tcctggctcg ggactggccg acccgcaccg ccttctcggt
     4561 cttgcgctgc atcatgcgcc ggatgaactc ctcttggcgc ctcaggtagc gctgttggac
     4621 ctcgtaggct ttggcttggg cctgcagttc gcggtccttg gcttcggcgt acgcggtgta
     4681 gccgccggcg tgttccgaga gcacgccatc ctcgacctcg gcgatcctct cggcgacggc
     4741 atcgagaaag tagcggtcgt gggagatcgt cacgaaccca ccgtcgtagc tgcgtaggaa
     4801 ctcctggagc cactccatcg cctcgatgtc gaggtgattg gtgggctcat cgagcagaag
     4861 aaggtccgtc tcgtcgacca ggaccttggc cagcgccaca cggctgcgtt gtccccccga
     4921 gagatgcccg agcggtacgg tgtggtggtc ggctggtaga ccgagccccg ccagagtgcg
     4981 gccgatccgg gcctcgaact ccatgccgcc ggcgcgctga tatgattcga tggcctcgga
     5041 atgaagggtg agcaatctat cgcgagaacg gttcttgggg tcgagttccg gctcagagat
     5101 gagggccgca agctcctcga tatgacgctc gcactcgcga agatcgggcc ggcagagaag
     5161 catttcctcg tgtgcgctgc gttcgaggtc gagttccggc gattgcgcga ggaggccgat
     5221 gcggatgcca cggtgcagcg taacggtgcc ccggtccggt gtgcaggcgc ctgtgatgat
     5281 acggaagagc gtggatttgc cgacgccgtt ctcgcccacc actccgatct tctgcccccg
     5341 gtcgatctgc caggtgacgt cgcggagcac ctcattcgcg ccgaagcatt tggccacgtt
     5401 ctgtagacgc accagcacca ttcggagacc gcccttcccc tcttactgta tgcgccggcg
     5461 gccccggcac tcaaggcgag gacgatgtcg gacgaagggc agcgctctgg gtggatcgaa
     5521 gggacgtccc agactggaag ctggagacct ggccccatgc ccctgctcga gatgcggcag
     5581 atcagcaaga gctaccacgg tgtcccggta ctgcgcgagg tcgatctgga ggccgagggt
     5641 ggcgaggtgc atgccttggt cggcgagaac ggggccggca agtccacgtt gatgaagatc
     5701 ctcgacggag ccgtccgggc tgaccagggg tcgatccggc tcggcgggga gctcgtggac
     5761 atccccagtc cgcgtgcggc ccagcagctt ggagtggacg tgatccatca ggagctcctg
     5821 ctggccccct cgctggatgc cgccgagaac atcttcctgg gccgcacacc ctcgcggggt
     5881 cgagtgttcg tcgatcgtcg gacgctctac cagcgcgccg aggccgtcct ggccgagttg
     5941 ggtgccgatc tcgatgtgcg cgtgccgctg cgccgcctca ccgtggccca acgccagatg
     6001 gtggccatcg ctcgggcgct gtcagctgag gcgcgcatag tggtgatgga tgaaccttcg
     6061 gcagtactga ctcggaatga gctcgagcgc ttgttcgagg ccatcgggcg gctatgcgct
     6121 cgtggcgtgg ccgtggtgta catctcccac cggctcgacg aggtcttccg gctggccgcc
     6181 agagtgaccg tgctcaagga cggccagtcc caaggcacgt accccattgg cgagctgaca
     6241 cgggccaagg tgatccggct gatggtgggg cgcgacatcg aggtaacggt gtcgcgcacc
     6301 gatcagagcc ggccggtgga ggtgctccgc gtcgagggga tgcgcagcgg gcgcagggtc
     6361 cgggggtgcg atctgtcgct ccgccagggc gagatcctcg gcatcgcggg gctggtgggc
     6421 gcggggcgca ccgagctggc ccgggccatc gtgggcgccg accggcgcga tgcgggccaa
     6481 gtctatgtcg acgggcggtc ggtgcacatc ggtagccccg cggaggcgat ccggcacggg
     6541 atcgcgtaca tcgctgagga ccgcaagacg tgcggcattt tccccgaact tggggtggcc
     6601 gacaatgtgt tgcttcccac ttatgagaac gcgtcgcgct ggggcgtggt cggccttggg
     6661 tctgcgcgcc ggaccgtcag ggcgctggtg gagcgcctgg acgttcgagc cgcagacctg
     6721 gcgcagcggg tggtcacgct cagtgggggc aaccagcaga aggtcgtcct cgcccgttgg
     6781 ctcaaccggc aggcccggat cttcatcttc gatgagccca cgcgcggcat tgacgtactg
     6841 gccaaggagc atatctatgg ccttatgaac cagctcattg atggtggagc gtcgattgtg
     6901 atgatctcat cggaactccc cgagttgttg gccatgagcg accgcatcct ggccatgcgc
     6961 gaggggctta tcgtgggtga gatctcccgg gccgaggcca cagaggagcg cgtgctggcc
     7021 atgtgcatgg ggcagatgca ggtggcgtag gcgtgcacgg ggccggccgg cccggcccgc
     7081 tggtcagccc ttctccttca gcaccttcag gccccgctgc gcgatgaact cggcgcagag
     7141 gccgaaggcc ttcatcagca cccagggatc gcccgattcg ccgaaaccac acacgccgat
     7201 ctgatcgatg agggcgccgg ccgacgcggg gtgccggctc accacaccgg ccacgatgtt
     7261 ccccagcccg ccagcct
//
LOCUS       DAOSPL010000026         7248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_10325_length_7248_cov_3.076602, whole genome shotgun sequence.
ACCESSION   DAOSPL010000026 DAOSPL010000000
VERSION     DAOSPL010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10325_length_7248_cov_3.076602"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PLQ54_00930"
     CDS             <1..845
                     /locus_tag="PLQ54_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOS91852.1"
                     /translation="GSMPPVAKTMAAGGIEPSEIAALGIGAPGPVDRADGTVLEMPNI
                     PVGRLPLRAELQRHYPVPVVVDNDVNAGTFGEFWRGAGRGTRHMVGVFVGTGTGGGII
                     IDGEILRGKTGITGEIGHIVLDPDGPECGCGRRGCLEALASRTAMQRRVEAALRDGRK
                     SVLLDAAGGDLAGLRSKAFAKALSRGDALAEEVIRDCAIHLGYGAVALVHLLSPECIV
                     FGGGVMESLAPVMIPVIEGVVRERCLKGTAKGIGILPAKLGDHAGVLGAAALAMKELG
                     TLSA"
     gene            949..3369
                     /locus_tag="PLQ54_00935"
     CDS             949..3369
                     /locus_tag="PLQ54_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91853.1"
                     /translation="MPAGARHVSFRFAGPDIETPIGVLGAPGATYVLPDGSLRAADER
                     ISFRQADGRPLTADATVRQSALDGYLPMVVSDTRVGGVPVALTAFAVDLPGVGTCDVV
                     RVAAGPSRGGPGPLVLRAEMTAGDGLRAEGARLTSDGRVRLAGLPGEGHPELAAEVEG
                     RAAGHFGEYHGVGTFWPRPEGQYSPVFATMCIGWRSQPMTYRFRVEPGGSYVVAVGFW
                     EWHWKERGHRLVETTIDGQAGGVVDTVSAGTGHPVVAQYAAHDADGNGLLDVTVAAAR
                     SAPDQNSILNVIWLFPAGSPPDGDALLRGDLDREALAMVDCGSLSDRPFSDAAIRLSL
                     PVAPGRPTTAWVVRPRDATRETASQLPGELDDGLIDRARQVWEAMLRDAVIPDLPHPV
                     ATDFYRASLAQILLQPDRPGPTAANALTYAGLFGHAERRLACCLDRQRGNTPSHEAAA
                     QWDGPGQAIWAFATHARLSGNWDFMARAYPDLMREVRQIAASRARSKRLDAQGERPPT
                     YGLLLLGTDDSDAPSAARYAHDFWTLYGIELVLSGARALGRTSDVAWLEAEREDFSSC
                     LTESVARSFVELPDGQGYIPSTPGSQEDAALWGNLAALYPTRFLLGDSRMARTMALLG
                     SRQAEGLPAGPGSASGGMAPRIACDVARCHMLLGEYDKAAELLYAILDHASPTRCWME
                     AETAAAYISMLRDMVAFEDGGTLRLASAVPRDWLLDGQSIAVRDLPTAVGRVSFRLRS
                     DATQGRMSADVRAPRGTPVVLHLRHPGLWRLTSVKLNGREWADHGTDWVRFAASGTPT
                     RVEARFEE"
     gene            3376..4158
                     /locus_tag="PLQ54_00940"
     CDS             3376..4158
                     /locus_tag="PLQ54_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS91854.1"
                     /translation="MTGACGPGDGVPLIECRGLGLSRGGHPVLRQVDLTVGRAEIVGV
                     LGRSGVGKSTLLRCIVGLERPDRGCVKLMGQDIGGLGEDELNSLRRQVGLVFQSAALF
                     DSLSVFENVAFYPRRFLRLTESELGELVARHLAVVGLPGTERLMPAQLSGGMAKRVGI
                     ARCLAAEPKVILYDEPTAGLDPVMAAVLDRVIVDLRDDLGISAVVVTHDVRNALTVVD
                     RAALLHGGTIYFEGRPDAFAETADTLVRQFVDGAVDGPLTDG"
     gene            4168..5244
                     /locus_tag="PLQ54_00945"
     CDS             4168..5244
                     /locus_tag="PLQ54_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91855.1"
                     /translation="MAGLAYRRDMDEVRQRLTLWWQGGDLGRPALHLYAPRPEPAEAV
                     PAMPEPPGWTGHYSTRDFDYRVNLALRECLGTYYLAEAVPCVLPDLAPNCLALYLGCR
                     GVEMPDTVWCEPCMDSPAEARFEYDPANFYWDFTLRLAREQLRLGAGKFLTGFPDLIE
                     GLDTLAAMRGTEPLLVDLTERPDWVRWCLRQITDRYFHYYDILYDLIRDEVGGSHFWA
                     WAPGRMAKFQCDFSAMISPDMFGEYMVPVLREMCERVAHSMFHWDGPGALGHHDHLLS
                     IPRLSMLQWTPGAGVEPVDHPRWWPLYHKTVEAGKKVFVNAASLDSLLAMKREFGDEL
                     KQFLIAMWVASPEQAAEVMRIAEV"
     gene            5249..5791
                     /locus_tag="PLQ54_00950"
     CDS             5249..5791
                     /locus_tag="PLQ54_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91856.1"
                     /translation="MPTASPASLLQEARAAFAAGDYDRALAQARAALDLTRTQAPGPH
                     GMEFLCRNLMARSLEARGDAREALALHWENARRGSDQEDTYQRLAALGERLGDAEAVR
                     VGCQWLQSVAAQFGLVDLRAKQRQERVERRLSQGRFGPAHVESDAAERPPQAATTGSS
                     CLVVLAACIALCLAAWFVNG"
     gene            complement(5800..6699)
                     /locus_tag="PLQ54_00955"
     CDS             complement(5800..6699)
                     /locus_tag="PLQ54_00955"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOS91857.1"
                     /translation="MRVRVVLVVLLVIALPAVALPPEASVPDRSADTVLVASFNIKWF
                     ARQTQDLHKIAQVIAHFDLCSIVELQSDDALPRLLQVLEAETGEAWMYICSHDTGDDA
                     RYLERYGFIWRPDRVRLASGFVGNLFQPADADIGDRHRHAPYTAHFRAGGFDFACIVA
                     HTVWSTVARRKREVALLGQELEMLRARTAERDLLLMGDFNYPVSSPAMASLIENLGLR
                     CLEPEGVGTTLKADGSAGESAYDHILVNASATVRDWTGRSGAYYFVPALYPGGEGAAR
                     ASSEVSDHIPVWAEFHTGLVDDD"
     gene            complement(6935..>7248)
                     /locus_tag="PLQ54_00960"
     CDS             complement(6935..>7248)
                     /locus_tag="PLQ54_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS91858.1"
                     /translation="GRAHGRLNRHLRRNADRVSVVIMDTTMPRMGGREAIRHIRDVRD
                     DVPLIVASGSMQPTEGDPVDDHIAAFLLKPYRPSEMVQRVREVIRARGEPRASTGAQE
                     V"
BASE COUNT         1083 a         2387 c         2548 g         1230 t
ORIGIN      
        1 atggctcgat gcctcccgtt gccaagacca tggccgcggg aggcatcgag ccatcggaaa
       61 tcgccgctct gggcattggg gcgcccgggc ccgtcgatcg ggcggacggc accgtgctcg
      121 agatgccgaa tatcccggtg gggagactgc ctctcagggc cgagctgcag cgccactatc
      181 ccgttcctgt cgtcgtagac aacgatgtga acgcggggac tttcggcgag ttctggcgcg
      241 gagctggacg tggtacacgc cacatggtgg gcgtgttcgt cggaacgggc acgggcgggg
      301 gtatcatcat cgacggcgag atcctccgtg ggaagacggg tatcacggga gagatcggcc
      361 acattgtgct ggaccctgac gggcccgagt gtggatgcgg gcgccgaggt tgcctggaag
      421 ccctggccag ccgaaccgcg atgcagcgac gagtcgaggc cgcgttgcgg gatgggcgta
      481 agtcggttct gctggatgcc gccggtggcg acttggcggg tctccgcagc aaggccttcg
      541 ccaaagcgct ctcgcggggc gatgctctgg cggaggaggt gatccgcgat tgcgccatcc
      601 acctcggtta cggtgcggtg gcgctggtcc atctgcttag ccccgagtgc atcgtcttcg
      661 gcggcggagt catggagtcg ctcgcgccgg tcatgattcc ggtcatcgag ggcgtcgtcc
      721 gtgagcggtg cctgaagggc acggccaagg ggatcggaat ccttcccgcg aaactgggag
      781 atcatgcggg tgttctgggc gcggcggcac tggccatgaa ggagctcggc acgctgtccg
      841 cctagactcc gcgccatgct gtctcgccgg ggagtgtccg cgttgtctcg ggtatgttgc
      901 cgacgggcat cggtctgggt gttggctgtc gcctgcactg ctgtcaccat gccggcgggg
      961 gctcggcatg tctcgttccg cttcgccggc ccggacatcg agacgccgat tggcgtgctc
     1021 ggtgcccccg gggccacata cgtgctgccc gacggatcgc tgcgtgcggc ggacgagcgg
     1081 atcagcttcc gacaggccga tgggcgtccc ctgactgccg atgccacggt tcggcagtca
     1141 gcactggacg gttacctacc gatggtagtt tccgacaccc gggttggcgg cgtgccggtc
     1201 gcccttacgg ctttcgccgt cgacctgccc ggcgtgggga cgtgtgacgt cgtccgggtg
     1261 gccgccggac catctcgagg aggtcctggg ccgctcgtgc tgcgggccga gatgacagcg
     1321 ggtgacgggc tgcgtgccga gggcgcccgc ctgacgagcg acgggcgggt ccgtcttgcc
     1381 gggctgcctg gcgaggggca ccccgaactg gctgccgagg ttgagggccg cgccgccggc
     1441 cacttcgggg agtaccatgg cgtcggcacg ttctggcccc gacctgaggg gcaatacagc
     1501 cccgtcttcg ctaccatgtg catcggctgg cggagccaac cgatgaccta ccggtttcgt
     1561 gtcgaaccgg gcgggtcata cgtcgttgcg gtgggcttct gggagtggca ctggaaggag
     1621 cgggggcacc gcctcgtaga gaccaccatc gacggccagg ccggaggggt ggttgatacc
     1681 gtctcggccg gaaccgggca cccggtggtg gctcagtacg cggcccatga tgccgacggc
     1741 aacggattgc tcgacgtgac cgtcgccgcg gcgaggagcg cgccggacca gaactccatc
     1801 ctgaacgtga tctggttgtt ccccgcgggc tcgccccccg atggggatgc tctgctccgc
     1861 ggcgacctgg accgcgaggc actggccatg gtcgactgcg gcagcctctc cgaccgcccc
     1921 ttctcggatg ccgcgatccg gctgtcgctg ccagttgctc ccggccgacc cacaaccgca
     1981 tgggttgtgc ggccccggga tgccacccga gagacggcga gccagctccc cggcgaactc
     2041 gacgacggcc tgatcgacag ggcgcgacag gtatgggaag cgatgctccg cgacgcagtg
     2101 atcccggacc tgccacaccc agttgccacc gacttctacc gggcctcgct ggcccagatc
     2161 ctcctccagc ccgatcgccc tggacccacg gccgccaacg cgctgacgta cgccggcctg
     2221 ttcggccatg ccgaacgccg tctcgcctgc tgcctggatc ggcagcgcgg aaacacaccc
     2281 agccacgagg ctgcggcaca gtgggacggg ccgggtcaag ccatctgggc cttcgcgacc
     2341 cacgcccgtc tctccggcaa ctgggacttc atggcgagag cttatccgga tctgatgagg
     2401 gaagtccggc agattgccgc gtcccgagcc cggtcaaagc ggctcgacgc ccagggcgaa
     2461 cggccgccga cctatggcct gctcctgctc ggcaccgacg acagcgatgc gccgtcggcg
     2521 gcccgctatg cgcacgactt ctggacgctg tatggcatcg aactggtcct gagtggcgcg
     2581 cgcgcgcttg gtaggacctc cgatgtggcc tggctcgagg ccgagcgcga agacttctct
     2641 agttgtctca cggagagcgt ggcacgaagc ttcgtggagc ttcccgatgg tcagggctac
     2701 atcccatcga ctcccggcag tcaggaggat gctgcgctct ggggcaactt ggcggcgctc
     2761 tacccgaccc ggttcctgct gggagacagc cggatggctc gaaccatggc attgcttggg
     2821 tctcgccagg ccgaggggct gcccgctggg cccggctcgg cctccggcgg catggcgcca
     2881 cgcatcgcgt gcgatgtcgc ccgctgccac atgcttcttg gggagtatga caaggcggcc
     2941 gagctcctgt acgccattct cgaccatgcc tcgcccacgc ggtgctggat ggaagcggag
     3001 actgcggccg cctacatcag catgctccgg gatatggttg ccttcgagga tggcggcaca
     3061 ctgcggctgg cctcggccgt gccacgcgac tggctgctgg acggccagtc catcgccgtc
     3121 cgcgacctgc ccaccgccgt gggacgcgtg agcttccgcc tgaggtccga cgcgacgcag
     3181 ggccgcatgt cggctgacgt tcgtgcaccc agggggactc ccgtggttct gcacctccgg
     3241 cacccggggc tctggcgact gacaagcgtg aagctcaacg ggcgcgagtg ggccgaccat
     3301 ggtaccgatt gggtccgctt tgcggcgagc ggaactccga cgcgcgttga ggcccgcttc
     3361 gaggagtagc ggaccatgac cggcgcctgt ggccccggag atggcgtgcc cctcatcgag
     3421 tgccgcgggc tcggtctgtc gcgcggcgga cacccggttc tccgccaggt cgacctgacg
     3481 gtaggccgag ccgagatcgt gggggtcctg ggccggtcgg gtgtcgggaa gagcaccctg
     3541 ctgcgctgca ttgtcggtct ggagcggccc gacaggggct gtgtgaagct gatggggcag
     3601 gacatcggcg gccttggtga ggacgagttg aactcgttgc gccgccaggt cggtctggtg
     3661 ttccagtcgg cggccctgtt cgactcgctc tcggtctttg agaatgtggc cttctacccg
     3721 cgccggtttc tgcggctaac ggagtctgag ttgggtgagc tcgtcgcgcg ccacctggca
     3781 gtggtgggat tgccgggcac cgagcggctc atgccggcac agctctcggg cgggatggcc
     3841 aagcgagtgg gcatcgcccg gtgtttggcg gccgagccca aggtgattct gtatgatgag
     3901 ccgacggcgg gcttggaccc ggtgatggcc gcggtactcg atcgcgttat cgtcgatctg
     3961 cgcgacgact tggggatctc ggcagttgtg gtcacccacg acgtgaggaa cgccctgacg
     4021 gtggtggatc gggccgcgct cctccacggc ggcacgatct acttcgaggg gcggccggac
     4081 gcctttgcgg agacggccga cacgttggtg cggcagttcg tggacggggc cgttgatggt
     4141 ccactaaccg acggatgagg gtaccacatg gcaggactcg cgtaccgccg tgacatggat
     4201 gaggttcggc agcggttgac cctctggtgg cagggggggg acctgggacg cccggcgctt
     4261 cacctctacg cgccccggcc ggaaccggcg gaggcggtcc ccgcgatgcc agagcctccc
     4321 ggctggacgg gccactactc gacgcgcgac ttcgactacc gtgtcaacct ggccttgcgc
     4381 gagtgcctgg ggacctacta cctggccgag gccgtcccgt gtgtgctccc cgacctggct
     4441 cccaactgcc tggcactcta cctcgggtgc cgaggcgttg agatgcccga cacggtctgg
     4501 tgtgagccct gcatggattc acccgccgag gcccgcttcg agtacgaccc ggccaacttc
     4561 tactgggact tcaccctccg cctggctcgc gagcagctgc gcctgggagc tggcaagttc
     4621 ctgacgggct tcccggacct catcgaaggc ctcgacactc tcgctgccat gcgggggacc
     4681 gaaccgcttc tcgtcgacct gaccgagcga ccggactggg tccgttggtg cctccggcag
     4741 atcaccgatc gttatttcca ttactacgac atcctctatg acctgatccg cgacgaggtg
     4801 ggtggttcgc acttctgggc gtgggcgccc ggccgtatgg ccaagttcca gtgcgacttc
     4861 tccgccatga tcagcccgga catgttcggg gagtacatgg ttcccgtgtt acgcgagatg
     4921 tgcgagcgcg tggcccactc gatgttccac tgggacggcc cgggcgccct tgggcatcac
     4981 gaccatctgt tatccatccc gcgtctgtcg atgctccagt ggacgcctgg cgcaggagtt
     5041 gagcccgtgg accacccgag gtggtggccg ctctaccaca agacggtgga ggcagggaag
     5101 aaggtcttcg tcaatgccgc gtcgctcgac agcctattgg caatgaagcg ggagttcggt
     5161 gacgagctca agcagttcct gattgccatg tgggtggcct caccggagca ggcggcggag
     5221 gttatgagga tcgccgaggt atagtgccgt gccaaccgcg tcgcccgcat cgctgttgca
     5281 ggaggcccgg gcggccttcg ccgccggaga ctacgaccgg gcgctggccc aggccagagc
     5341 cgcgctcgac cttacccgca cccaggctcc cggcccgcac gggatggagt tcctgtgccg
     5401 gaacctcatg gcgcgatcgc tggaggctcg cggcgacgcc cgggaggcgc tggccctcca
     5461 ttgggagaac gcgcgccgcg gctccgacca ggaggacacc taccagcgcc tggccgcact
     5521 gggcgagcgg ctgggcgatg ccgaggcggt ccgcgtcggc tgccagtggc ttcagtcggt
     5581 ggcggcgcag tttgggctcg tggacctcag ggccaagcag cggcaggaac gcgtcgaacg
     5641 acgcctctcc cagggcagat tcggtcccgc gcacgtcgag tctgacgcgg ccgaacggcc
     5701 accgcaggcc gcgacgaccg gttcgagctg cctcgtagta ctggcggcgt gcatcgccct
     5761 ttgcctggcg gcctggttcg tcaacggttg atccccgagt cagtcatcat caaccaggcc
     5821 agtgtggaac tcggcccaca cgggaatgtg gtcggacacc tccgagctgg ctcttgccgc
     5881 gccttcgccg cccggataga gggccggcac gaagtagtag gcccctgagc ggcccgtcca
     5941 atcccgaacc gtggcgctcg cgttgaccag gatatggtca taggcactct cgccggcgga
     6001 accgtcggcc ttgagagtag ttcctactcc ctcgggttcg aggcaacgca agcccaggtt
     6061 ctcgatgagg gaggccatgg ccggtgagct gaccgggtag ttgaagtcgc ccatgagcag
     6121 caggtcccgc tcggccgtac gcgcccgcag catctcgagc tcctggccca gcaacgccac
     6181 ctcgcgcttg cggcgggcca cggtactcca caccgtgtga gccacgatgc aggcgaagtc
     6241 gaacccaccc gcgcggaagt gggccgtgta tggcgcgtgc cggtgacggt ccccaatgtc
     6301 tgcgtccgcc ggctggaaca ggttcccgac gaagccgctg gccagacgga cccggtcggg
     6361 ccgccatatg aagccgtacc gctcgaggta tcgggcatcg tcacccgtgt cgtggctgca
     6421 gatgtacatc cacgcctcgc ccgtctcggc ctcgaggacc tgcaggagcc gcggcagggc
     6481 atcatcggac tgaagctcca cgatgctgca caggtcgaag tgggcgatca cctgggcgat
     6541 cttgtgcagg tcctgcgtct ggcgagcgaa ccacttgatg ttgaacgacg ccacgagaac
     6601 ggtgtcagcg gaccggtccg gcacactggc ctcaggcggc agggcgactg cggggagcgc
     6661 aatcacaagc agcacaacca gcacgacccg gactcgcatg tggacagcct cccctcgggt
     6721 ggttccgcgg cgactctcgg gcagagggca gttccaggct tcggcccaga ccgtaagggg
     6781 tcctccgcac caggcggcaa ccacgccgag gccaccctcg gcctggcgcg gaacagggta
     6841 gggggggcgg cggactaccc agaggccgga tcaggcgagt gggcgcgagc cgcagccagg
     6901 acaacgggcg gccgacaggc ggggccttct gcccctatac ctcctgggcg cccgtactgg
     6961 cccggggctc cccacgggcc cggatgacct cccggacgcg ctgcaccatc tcgctgggcc
     7021 gatatggttt gagcagaaac gccgcgatgt ggtcgtctac cgggtctcct tcggtcggct
     7081 gcatcgagcc cgaggccacg atcagcggca cgtcatcgcg cacgtcgcga atgtgccgaa
     7141 tggcctctcg gccgcccatg cggggcatcg tcgtgtccat gatcacgacg ctgactcgat
     7201 cggcgtttcg gcggaggtga cggttcagtc gcccgtgagc gcggccat
//
LOCUS       DAOSPL010000027         7097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_10616_length_7097_cov_3.248083, whole genome shotgun sequence.
ACCESSION   DAOSPL010000027 DAOSPL010000000
VERSION     DAOSPL010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10616_length_7097_cov_3.248083"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1924)
                     /locus_tag="PLQ54_00965"
     CDS             complement(<1..1924)
                     /locus_tag="PLQ54_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91859.1"
                     /translation="MAWRLALVLLAAAVGTASGAEPIRIALDRCTVEWSEADGVSVLY
                     DDQLVLGGDPQPVYPHPPDWSWGYRPAAPVTAALERRGDTQVLRLQCADAKVRWTQTV
                     TAGPGDRVRVAYSYRQLAWDEAVSVQVCLFRPAPSWLAGAEFHATGPGGKASGRIPDD
                     YESPHPFGAATRAELLGPSMGLTLEASRPLMLFDYPARNHFWLGCDEPLPRGEERGWS
                     ADLAFEGRAIVVRGVRLEGFRLPSHTDGERLGCLVRLGREAEGPGAVTCRLVEQAGEP
                     VRLAEQVVALSRETQAVPLAADVGRPGKHRVRLELLLGTEVVYTSTPVAVEVPRVMMI
                     APGRSPYMAETRGEALVRLVGAIEGDLRVAVEGPGGPLHGGPIRVGETVAVPLDLGAF
                     PPGHSELSATLYRGTVRLGSARCDLYRLPPKDGAVVIDNRSHGLVCGGLPFIPVGMYT
                     DFGIARRLAEVEAPYGFTVIGPYMSPDIAERRQMRDQVRALFDRCAALGMYVHFDIRA
                     ASRPPQTEETWAWLEEEITAFRDHPALLAYYLADEPELGWAKPENTTEAYRRIKELDP
                     YHPVTMVFCQSGAAAEYADGMDLIMTDPYPIPNGPITQVVDFCERIRTDTLDVKPLWL
                     VPQAFGGGEWWAREPSR"
     gene            2140..3033
                     /locus_tag="PLQ54_00970"
     CDS             2140..3033
                     /locus_tag="PLQ54_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91860.1"
                     /translation="MRPSISAAAAVLTACAMAWADGPPDRPSAEDVARGLAGPEGWAV
                     ADLHVYGPDNVYEKIDGQDQAYLAFDLVAMANVLFEKDKDGLDVEVYDMGKPEHALGI
                     FAQQHRAPNTQYETIEGGEAAVFPARIFAWKGPFYVRIDRRRKAAEDEDGLVRRLAHS
                     LLRAIPGRADGAGLVAPLPKQGMRPHSAGFTHKDYLGYECLSDVRQAKYSPSDRPVTL
                     FLLRPADAVVAMSAVRQGEKSIEDVPGLGDEAFRAKRPYFGTICVVRLGKFIVGSVDP
                     EGTAGAPEALRETLRMLGQRR"
     gene            3052..3732
                     /locus_tag="PLQ54_00975"
     CDS             3052..3732
                     /locus_tag="PLQ54_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS91861.1"
                     /translation="MKRWALAAVVCMAGTAGAQVGVGEQPVPPPPDAVILFDGTGMSA
                     WAKQDSDEPVSWRLVDGAMEVAGGNIVTREHFGDCQLHVEFMVPLMPDATGQARGNSG
                     VYLQGSYEVQVLDSFGLELGLGDCGAIYGVAVPMVNACRPPGTWQTYDILFHAPRFDG
                     ANLVQNARMSVLQNGVWIHEYAEIPGPTTAAMHREDLSQPGPLMLQDHGCPVRYRNIW
                     VRPLTAAE"
     assembly_gap    3777..3786
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3787..4988
                     /locus_tag="PLQ54_00980"
     CDS             <3787..4988
                     /locus_tag="PLQ54_00980"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOS91862.1"
                     /translation="AQKRQALSYPKPTTAWRGEPQLGGWYTEEEIEAAVAAIRSSMDW
                     TGEGFGFIVQEILDFEAAFARYTGAKHAISLSTASVGLDLAMQALDLQPGDEVISPAM
                     NFRAAHHAIIGAGGRLVLCEVDPATLCADPADVERRVTPRTRAICVTHMNGRAAPVDD
                     YVEIGQRHPHPEHGPIPVIGDAARAVGVGYKGGRIGAGGWCTIFSFHTMKLMTTLGEG
                     GMVTTNNDALAERLTAARQWGGTTEYWGSSFKLTKVQAAVGLVQLRRLDEMNGLRIRR
                     ALERTELLKDVPELTLPPLPGECGHTYYLYTVFVPPEWAGEKRDRIRAIMLDEFAVGS
                     EVANPPTYDTSPHIRKHTEGQRLPATEFLANRMICVPIHPLMTEEQNAYVAAVLQEAV
                     ERVRTEG"
     gene            4993..6216
                     /locus_tag="PLQ54_00985"
     CDS             4993..6216
                     /locus_tag="PLQ54_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91863.1"
                     /translation="MGCVPAQERSLVRELASQVAMLANSDEYETRRRRWRDVNAMRRP
                     DRAPVWCRPAGAWKELVPPSSLQCTDPWLRSIEYALRQHLYKHWVGDDHIVDPWWTVL
                     AVWRCDSEHVWGLRTGELVESTELGGFRYGTPIRTEADFDKVTVPTFTYDHEATQDQA
                     NRVADLLDGIMPVRITCEPPITPSLGTILDKLRGMSDFMLDLALNPRLIHRLMAKILE
                     GTLRAMRVAEESGRLTPNHYGPMLCSEPLNGTPEPGQVKLHHLWGVANSQEFEEVSAD
                     MFDEFLLNYQIPILQQFGAVQYGCCENLTRKIDRVTRIPNLRVFVASAWTDLDRVIEA
                     CSGRYAIMWRQLASDVVFPDDMASIERHLDEGMAKLKGQSYQVVLREIETLAGHPNRL
                     REWARAAIALAEKHA"
     gene            6545..>7097
                     /locus_tag="PLQ54_00990"
     CDS             6545..>7097
                     /locus_tag="PLQ54_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91864.1"
                     /translation="MRSQRLARLCPQLVAFLVVVSGSPVLAIGHPHVDVPSEFIIGAA
                     ACVVPIYFHNANHQPTTYTGTVTASVTHDPMGFAEFTRNDPYWNGTAVVTQQNTYVQG
                     ARVSHTGATPPYGTSISFQVAGVSGTSVNLGVYSFGTAEVTVTAGGKSNSGSVSDIWI
                     QGRWCLCRRTTLPMTRTGITTGGR"
BASE COUNT         1223 a         2414 c         2334 g         1116 t
ORIGIN      
        1 ggcgcgaggg ctcccgagcc caccactcgc cgcccccgaa cgcctgaggc accagccaca
       61 acggcttcac atccagtgtg tccgtgcgga tgcgctcgca gaaatcgacc acttgcgtga
      121 tcggcccgtt tgggatgggg tagggatcgg tcatgatcag atccatgcca tcggcgtact
      181 cggctgccgc accagactgg cagaagacca tggtcaccgg atggtaaggg tcgagctcct
      241 tgatcctccg ataggcctcc gtcgtgttct cgggcttggc ccagcccagc tcgggctcgt
      301 cagccaggta gtaggccagc agggccgggt gatccctgaa agccgtgatc tcctcctcga
      361 gccatgccca tgtctcctcg gtctgcggcg gccgcgaggc cgcccggatg tcgaagtgga
      421 cgtacatccc cagcgccgcg cagcggtcga acagggccct cacctggtcc ctcatctgcc
      481 tccgttcggc gatgtcaggg gacatgtagg gaccgatcac ggtgaaccca tagggcgcct
      541 cgacctcggc gagtctccgc gcgatgccga aatcggtgta catccccacc gggatgaacg
      601 gcaggccgcc acagacgagg ccatggctgc ggttgtcgat cacaacagcc ccatccttgg
      661 gcgggagccg gtacaagtca cagcgcgcgc tccccaaccg cacggtaccc cggtatagcg
      721 tcgcgctgag ctcactgtgc ccaggaggga aggcgccaag atcgagcgga acagcgaccg
      781 tctcccccac acggatgggt ccaccgtgca gcggaccgcc ggggccctcg acagcgacgc
      841 gcaggtcgcc ttcgatggcg ccgaccagcc gcaccagtgc ctcgccccgg gtctcggcca
      901 tgtaggggct ccggcccgga gcaatcatca tcacgcgtgg tacctcgacc gcgacgggtg
      961 tcgacgtgta gaccacctcc gtcccgagaa gaagctccag tcgcacgcgg tgcttgcccg
     1021 ggcgtccaac gtcggccgca aggggaacgg cctgcgtctc gcggctcaat gcaaccacct
     1081 gttctgccag acggacgggc tctccagcct gctcgacaag tcgacaggtg accgccccgg
     1141 ggccctccgc ctcccgcccc agtcgcacca ggcagcccag tcgctcacca tcggtgtgcg
     1201 atggcaggcg gaagccctcc aggcgcacgc cgcgcacaac gatcgccctt ccctcgaatg
     1261 ccaggtcggc ggaccagccc cgttcctcgc cccggggcag gggctcgtca catccgagcc
     1321 agaagtggtt ccgggccggg tagtcgaaca gcatcagggg gcgactggcc tcgagggtga
     1381 ggcccatgga gggccccagc agttcagcgc gcgtggccgc gccgaacgga tgagggcttt
     1441 cgtagtcgtc cgggatgcgc ccggaggcct tgcctcctgg ccctgtcgcg tggaactcgg
     1501 ctcccgcaag ccatgagggt gctggccgga agaggcagac ctggacactg acggcctcat
     1561 cccaggccag ctgccggtag gagtaggcga cccggacacg atcgccagga ccggccgtca
     1621 ccgtctgcgt ccagcggacc ttcgcgtcgg cgcactgcag tcggagcact tgcgtgtcac
     1681 cgcggcgctc cagggcggcg gttacgggcg cggcgggccg gtaaccccaa gaccagtcgg
     1741 gcgggtgggg gtataccggc tgaggatcac cgccgagaac gagctggtcg tcgtacaaca
     1801 ccgacacgcc gtcggcctca gaccactcga cggtgcagcg gtccagggca atccggatgg
     1861 gctcggcacc cgaggccgta cccaccgcgg ccgccaagag gacgagtgcc agccgccagg
     1921 ccatgatgga cctcctcgtc tcgcgccgga gcgcgctcag atggggaatc agatcatgcc
     1981 cttccacggg cggacccggc ctgaccttcc acgacacccc gcctcgtcgg cgcatgcggg
     2041 tagcgcgcag gctacgacga cattccccgc ccgcgaaggg tctcccgctg ggttagcaga
     2101 acctcccacc cggaatactg gggcgggagg tgacccgtgg tgcgaccatc catttcggcg
     2161 gccgccgcgg tgctaacggc gtgcgcgatg gcgtgggcgg acgggccccc ggatcggccc
     2221 tcggccgagg atgtggcaag aggtctggcg ggccccgagg gctgggccgt cgccgatctt
     2281 cacgtctacg gccccgacaa cgtctatgag aagattgacg gtcaggacca ggcctacctg
     2341 gcctttgacc tggtcgccat ggccaacgtc ctgtttgaga aggacaagga cggcctcgat
     2401 gtcgaggtct acgacatggg gaagccggaa cacgccctgg gcatcttcgc ccaacaacac
     2461 cgggcgccta acacgcagta tgagacgatc gagggcgggg aggcggccgt gttccccgcc
     2521 cggatctttg catggaaggg tccattctac gtccggatcg atcggcgccg gaaggcagcc
     2581 gaggacgagg acgggctggt gcggcgcctc gctcacagcc tcctccgagc catcccgggc
     2641 cgagccgatg gggccgggtt ggtggctccc ctgcccaagc agggcatgcg gccgcacagc
     2701 gcggggttca cgcacaagga ctacctcggg tatgagtgcc tgtccgacgt ccgtcaggcc
     2761 aagtacagcc cgagtgatcg ccctgtgacg ctgttcctac tcaggcccgc ggatgctgtg
     2821 gtggccatgt cggccgtacg ccaaggcgag aagtccatcg aggatgtgcc gggcttgggc
     2881 gacgaggcgt tccgggcgaa acggccgtac ttcggtacga tctgtgtggt ccggttgggt
     2941 aagttcatcg tggggtcggt ggaccccgaa ggcaccgccg gagcgcccga ggcgctccga
     3001 gagacgctgc ggatgctggg tcagcggcga tgatggagtg aggtggtacc gatgaagcgc
     3061 tgggcgctcg cggcggtggt ctgcatggcc ggcacggccg gcgctcaggt cggcgtgggg
     3121 gagcaaccgg ttccgccacc gcccgatgcg gtcatcctct tcgacggcac gggcatgtcg
     3181 gcatgggcca agcaggactc ggacgagccc gtctcctggc ggctggtcga tggcgccatg
     3241 gaggtggccg gcggcaacat cgtcacccgg gagcacttcg gcgactgcca gctacacgtc
     3301 gagttcatgg ttccgctcat gcccgacgcc accgggcagg cccggggcaa cagcggagtg
     3361 tacctgcagg gctcgtacga agtgcaggtg ctggattcct tcggcctcga gctcggtctt
     3421 ggggactgtg gcgccatcta tggagtggcg gtacccatgg tgaacgcgtg ccggcccccc
     3481 ggtacgtggc agacgtacga catcctgttc cacgccccgc gattcgacgg ggcgaacttg
     3541 gtccagaacg cccgcatgag tgtcctgcag aatggtgtgt ggatccatga gtatgccgag
     3601 atccctggcc caaccacggc agccatgcac cgcgaggacc tgagccagcc cggtccgctg
     3661 atgctccagg atcatggctg cccggtgcgc taccgcaaca tttgggtgcg gcccctgacc
     3721 gccgcagagt agcgtcgcct gaacgcctcc cgacccccgc gcgcgaaagg aacccgnnnn
     3781 nnnnnncagc ccagaagcgc caagccctta gttaccctaa gccgaccacc gcctggcgtg
     3841 gtgagccgca gctcggcggc tggtacaccg aggaggagat cgaggcggcg gtggccgcga
     3901 tccggagctc gatggactgg accggcgagg gcttcgggtt catcgtccag gagatcctcg
     3961 acttcgaggc cgcgttcgcc cgctacaccg gagccaagca cgccatctca ctgagcacgg
     4021 cctcggtggg gctggatcta gcgatgcagg ccctcgatct ccagcccggc gacgaggtca
     4081 tctcgcccgc catgaacttc cgtgccgccc atcacgcgat cattggcgcg ggcgggagac
     4141 tcgtgctctg cgaggtggat ccggccaccc tctgcgcgga ccccgccgac gttgagcgcc
     4201 gcgtcacacc gcgcactcgg gccatctgcg tgacccacat gaacggtcgc gcagcacccg
     4261 tcgatgacta tgtcgagatc ggccagcgcc acccgcaccc ggaacacggt cccatccccg
     4321 tcatcggcga cgctgctcga gccgtgggcg ttggctacaa ggggggccgg atcggcgccg
     4381 gcgggtggtg caccatcttc agcttccata ccatgaagct catgaccacg ctgggcgagg
     4441 gcggcatggt caccaccaac aacgacgcgc tggccgaacg gctgaccgcc gcgcgccagt
     4501 ggggtgggac gaccgagtac tggggcagca gcttcaaact caccaaggtc caagcggccg
     4561 taggcctggt ccagctccgg cgtctcgatg agatgaacgg cctgcgcatt cgccgagccc
     4621 tcgagcggac cgaactgctc aaagacgtgc ccgagctgac cctaccgccg ctgcccgggg
     4681 agtgtgggca cacgtactac ctctacacgg tgttcgtgcc ccccgagtgg gcgggggaga
     4741 agcgcgaccg gatccgcgcc atcatgctcg acgagttcgc cgtgggctct gaggtggcaa
     4801 acccgcccac ctacgacacg tccccccaca tccgcaaaca caccgagggg cagagactcc
     4861 cggccaccga gttcctggcc aaccgaatga tctgtgtgcc gatccatccc ctcatgacgg
     4921 aggagcagaa cgcctacgtg gccgcggtcc tgcaggaggc cgtcgagcgc gtccggaccg
     4981 agggatgagc cgatgggttg cgtgcccgca caggagcgct cactggttcg ggagctcgca
     5041 agccaggtgg cgatgctggc gaacagcgac gagtacgaga ccaggcgccg gcgatggcgc
     5101 gatgtgaacg ccatgcgccg ccccgaccgg gcgcccgtgt ggtgccgtcc ggctggcgcc
     5161 tggaaagagc tggtgccccc gagctcccta cagtgcaccg atccatggct ccggagcatc
     5221 gagtacgccc tccgtcagca cctgtacaaa cactgggttg gcgacgacca cattgtggac
     5281 ccatggtgga ccgttctcgc agtctggcgc tgcgactccg agcatgtctg gggcctgcgc
     5341 accggggaac tcgtcgagag caccgagctg ggcggtttcc gctacggcac acccatccgc
     5401 accgaagcgg acttcgacaa ggtcacggtc cccactttca cctatgatca cgaggcaacc
     5461 caagaccagg cgaatcgggt cgccgatctg ctcgatggga tcatgcccgt ccggattacc
     5521 tgcgagcccc caatcacacc ctcattgggc accatcctcg acaagctccg ggggatgagc
     5581 gacttcatgc ttgacctggc gctcaacccg cgactcatcc atcggctgat ggccaagatc
     5641 ctcgagggaa cacttcgagc gatgcgcgtg gccgaggagt caggccgcct cacccccaac
     5701 cactatggcc cgatgctttg cagcgaaccg ctgaacggca cccccgagcc cggccaggtc
     5761 aagctgcacc acctctgggg cgtggccaac agccaggagt ttgaggaagt ctccgccgac
     5821 atgttcgacg agttcctcct gaactaccag atccccatcc tgcagcagtt cggtgctgtg
     5881 caatacgggt gctgcgagaa cctgacgcgg aagatcgacc gggtgacgcg cattcccaac
     5941 ctgcgggtgt tcgtggcctc ggcatggacc gacctcgatc gggtgatcga ggcctgctcg
     6001 ggccggtatg ccatcatgtg gcggcagttg gccagtgatg tggtgttccc cgacgacatg
     6061 gcgtcgatcg aacggcacct cgacgaggga atggccaagc tcaaggggca gagctatcag
     6121 gtggtgctcc gagagatcga gaccctcgcc gggcacccga atcgcctgcg cgagtgggcg
     6181 cgagcggcta tcgcgctggc cgagaagcac gcctagggcc tccgcgctgg gggcgttctt
     6241 caggacggat caggccgtcg gcgcggggcc ccgctccagc caaacgccga cccggctgcc
     6301 tgcgctctca ggaagcacag cgccaagact gcggggctct gtcccgcacc atggcagggc
     6361 taagcccgca gctctgacgc ttccgctcct caggcgcgag gcccgcccat caatgatctt
     6421 gtgtgctttt tttttaccat tcccgccggt tccagaggtg tgcgccccgt ccccgcgaac
     6481 aggccccgcg caagataggt atgcgtagct actttgtgag actgacggat gagctggagc
     6541 agacatgagg tcacagaggc ttgcccggtt gtgtccgcag cttgtcgcct tcctcgttgt
     6601 ggtgtctggc tcgcctgtgc tcgcgatagg ccaccctcac gtcgatgtgc catcggagtt
     6661 catcatcggg gccgctgcct gtgtagtacc catctacttc cacaacgcca accaccagcc
     6721 gactacgtac acagggacgg tcaccgccag cgtcacccat gatcccatgg gtttcgcgga
     6781 gttcactcgc aacgacccgt actggaacgg cacggcggtg gtcactcagc agaacaccta
     6841 cgtccaggga gcgcgggtga gccacaccgg cgccacgcct ccctacggga caagcatctc
     6901 cttccaggtg gccggtgtct ccggcacgtc cgtcaatctt ggcgtctaca gcttcggtac
     6961 ggccgaggtt accgtcacgg caggcggcaa gagcaactcg ggctcggtgt cggacatctg
     7021 gatccagggc cgatggtgcc tttgtcgccg cacgaccctc ccgatgacca gaactggaat
     7081 cactactggg ggtcggg
//
LOCUS       DAOSPL010000028         7015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_10789_length_7015_cov_3.514080, whole genome shotgun sequence.
ACCESSION   DAOSPL010000028 DAOSPL010000000
VERSION     DAOSPL010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10789_length_7015_cov_3.514080"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1396)
                     /locus_tag="PLQ54_00995"
     CDS             complement(<1..1396)
                     /locus_tag="PLQ54_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91865.1"
                     /translation="MDRSRWNGHRSALATLLGCVIAAGPAGAQAGAGPRTGDQGPPEP
                     ALPPAAILCESDPAGAEIWVGHPNGLPEFRKGVTPAEITIDSTTAFPADWRITLAKPG
                     YDPYSQVVTAVRGDRLSVRGDLERTCKVAFLKPNPETARWELLVQGYDGTRRRAVAQL
                     PPPLPGDGLSWAPTGRRLAYSSAGNIYMLSLDDPKPVAVTDSTAAEWAGSIFDSPAWS
                     PDGQLLAFRRLKAGTGQCSLCVAPVRADKAQPIVEVASGIVYTGAWSPASGFRRIAAE
                     ATDGLMVITLDPKGRALGDSVRIPQAGQPAWSPDGEHLAYAFDGQIGVADADGANPRP
                     LSQFAAGRAHSPCWSPDGGSLAVLLRYRPPDGYERDDIWLFDVQTGTQPRRVGLDPGQ
                     PGHGQCCRLAGFSPDGSSVLYEVGPRECADVWALEPVQKTTSLALAQASVPVWFGALS
                     EARFRNFALVRSHLF"
     gene            complement(1480..2925)
                     /locus_tag="PLQ54_01000"
     CDS             complement(1480..2925)
                     /locus_tag="PLQ54_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOS91866.1"
                     /translation="MAALCGCSTTTAPVDTGGFRFTVDFPQVEAALVPTGSQSVRFVI
                     KTKGTQVVVGTTLATRSNPTVSVGGLPAGLECIIRASAYPTAEGSGIMQATVEVERTV
                     GSGIQDVPFTMVSTVAQVSVSAPGPTVFVGGSSQLTATARNASNAIVIVGASAWRWTT
                     SDAAVATVASNGVVTGVGAGTVTITATDTESGISGSALLHVTTTADQIAFHSNRSGSA
                     QIYVMDADGSNLTRITSNTANDRGPVWSFAGDRMAFYSDRDGNWEIYTMAADGSAPVR
                     ITNDPADDFRPCWSPDGTRIAFYSNRSPDASWDIYVAHADGSGLTNLTANAADDQYPA
                     WSPDSTRIAFASKRDGPLHIYVMSASGANPTRLTDTQYDDWAPSWSPDGTRIAFQRVY
                     AAGAGYNWEVCTMNADGTSIVRLTDYPGDDKFPTWSRDGSRIVFTRDIDPGDGEDFEV
                     FSMRATDGGDLRNLTGNASADERPNWCWAPQ"
     gene            complement(3067..4527)
                     /locus_tag="PLQ54_01005"
     CDS             complement(3067..4527)
                     /locus_tag="PLQ54_01005"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOS91867.1"
                     /translation="MADIGVAGEVTPIGETPEAVEERRAVAPPPPGRVARVRQRAALA
                     LMVYDFIAASVILWAATVIAPPGDLPALTIPGVPAWLLGAVTFVFLAANGLAYQSRWV
                     ASAHATFKDLFIAAAGALGVLTLCASTNHVVKPVAFLLLAIVSSLARQFAVRFSGDLL
                     PERVLILGVNEHSIEAAQTLPFVEEPRFAVVGYVDDRQDPGRHPVAVWPILGRFADAA
                     NLATQHQVDLVIAALPATGRSRAVPIVPALAATRTKVIWMEDFYARYFHKIPVHYVDS
                     AWALYGLNQQRSLIYAAVKRLVDVAMATAGLLLAAVLYPLIAGLNALLSPGPVIYSQV
                     RVGRKGRPFRIYKFRTMVRNAEETGAQFAQKDDPRITPVGRLLRKTHLDELPQCWNVL
                     LGHMSMVGPRPERPEFVETLERQIPYFRLRHLVRPGVTGLATVRNGYAATLEESIRKT
                     EYDLYYIKQRNVWLDLSLMLETIVICFMGRNGQASN"
     assembly_gap    5134..5143
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5144..6609)
                     /gene="dnaX"
                     /locus_tag="PLQ54_01010"
     CDS             complement(<5144..6609)
                     /gene="dnaX"
                     /locus_tag="PLQ54_01010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOS91868.1"
                     /translation="MAYQSLYLKYRPQTFDEIIGQDHICRTLRNALSAGKPAHAYLFC
                     GPRGTGKTTTARILAKALSCEKGVTPDPCTTCDACQAIRDGSLLDVVEIDAASNTGVD
                     NIRELKEVIGYAPSWGRFRIYIIDEVHMLSTAAWNALLKTLEEPPDHAYFIMATTDPQ
                     KVLPTILSRCQRFDFRRVAQPQLVEHLTRIAERESIAVDEAALWAVARLSDGCVRDSI
                     TMFDQIRAYAEGQVTIDAVHAVLGTLDDEVFFRIMDQVAAADAAAVFHLVDGLAHEGK
                     DFAQVIHGLTGHCRNLLLVKSGCTSASALEASEDTVARLRRQAEGYTMDRLVDCLREL
                     SDARENLKWSHQHRLLLEMSLVGMMAPARAPSSEAAPLAQWPEPPPPAVPAQPACGLD
                     DDPPVLMPPPARAAAPARATDHWQKVVLRVAESMPRLGGILASSVAERVGEDELLITF
                     GSKFGCDSFSERLRNEEFGPSIRAAIKEKYALISESALG"
     gene            6705..>7015
                     /locus_tag="PLQ54_01015"
     CDS             6705..>7015
                     /locus_tag="PLQ54_01015"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91869.1"
                     /translation="MGPDPVSRAARPCFATVLISRRSATQDLTLTYAVPERLRGRLRP
                     GVPVLVPLRRGRAVAHVLEVDSEAPPEGTRARDLIDVVEDAPDIPAELLDLAQWVAAE
                     TR"
BASE COUNT         1153 a         2243 c         2485 g         1124 t
ORIGIN      
        1 ggaacaggtg ggagcggacg agcgcgaagt tgcggaacct ggcctcggac agcgcgccga
       61 accagaccgg cacgctggct tgggccaggg ccagcgaggt cgtcttctgc acgggctcga
      121 gcgcccacac atcggcacac tcgcgagggc ccacttcgta cagcacgctg gacccatcgg
      181 gtgagaagcc ggcgaggcgg cagcactgcc cgtggccggg ttggcccggg tcgagcccga
      241 cgcgccgggg ctgggtgccc gtctggacgt cgaagagcca gatgtcatcg cgctcgtatc
      301 catcgggggg ccggtaccgc agcaggaccg ccagggagcc gccgtcgggc gaccagcagg
      361 gcgagtgagc tcgacccgcc gcgaactggc tcagcggacg cgggtttgcg ccatcggcgt
      421 cggccacgcc gatctggccg tcgaaggcat aggccaggtg ctcgccatcg ggtgaccacg
      481 cgggctggcc cgcctgaggg atcctcaccg agtcgcccag ggcccgaccc ttggggtcga
      541 gtgtgatgac catgagcccg tcggtggcct cagcggcgat ccgccgaaag cccgatgctg
      601 gggaccaggc tccggtgtag acgatccccg aggcgacctc gacgatgggc tgggccttgt
      661 cggcacgcac cggcgcgaca cagaggctgc actgacccgt ccccgccttc agccgccgga
      721 aggccagaag ctgaccatca ggggaccagg cggggctgtc gaagatggag cccgcccact
      781 cggcggccgt ggagtcggtc accgctacgg gtttggggtc gtcgaggctc agcatgtaga
      841 tgttccccgc cgaggagtag gcgagccgcc ggccggttgg ggcccacgag agcccgtccc
      901 ccggcagagg cgggggcagc tgggccacgg cgcgccggcg ggtgccgtca tagccctgaa
      961 ccaggagttc ccagcgagcg gtctctggat tgggcttgag gaatgcgacc ttgcaggtgc
     1021 gttcaaggtc tcccctgacg ctcagccggt caccccggac ggcggtgacc acctgggaat
     1081 agggatcgta gccgggcttg gcgagggtga tgcgccagtc cgctgggaag gccgtcgtgc
     1141 tgtcgatggt tatctcggcc ggtgtgacgc ccttgcggaa ctcgggaagc ccgttggggt
     1201 gccccaccca gatctcggcg ccggcaggat cgctctcgca caggatggca gcggggggga
     1261 gtgccggctc agggggtccc tggtctccag ttcgaggccc ggctccggcc tgggccccag
     1321 ccggcccggc ggcgatgacg cagcccagca gggtggccag ggctgaccga tgaccattcc
     1381 atcgcgagcg atccatcgcc gtttccccaa cagggcaccc aggttcccgg gagctctcag
     1441 cagtcggttc cgatgataga ggagatgggc gtcaggggtc tactgtgggg cccaacacca
     1501 gttgggcctc tcgtccgcac tggcgttccc ggtgagatta cgcaggtcgc cgccatcggt
     1561 ggcgcgcatg gagaacacct cgaagtcctc gccatcgccc gggtcgatgt cgcgggtgaa
     1621 gacgattcgc gacccgtcgc gcgaccatgt cgggaacttg tcgtcgcccg ggtagtcggt
     1681 cagccggacg atgctagtcc cgtcggcgtt catggtgcag acctcccagt tgtagcccgc
     1741 gccggctgca tagacgcgct ggaaggcgat ccgggtgcca tcgggtgacc agctcggcgc
     1801 ccagtcatcg tactgggtgt cggtgagccg agtggggttc gctccgctgg ccgacatgac
     1861 atagatgtgc agcgggccat cgcgctttga ggcgaaggcg atgcgcgtgc tgtcgggcga
     1921 ccacgccggg tactggtcat ctgccgcgtt cgcggtgagg ttggtcaggc cggagccatc
     1981 ggcatgggcc acgtagatat cccaactggc gtccggactc cggttggagt agaaggcgat
     2041 cctggtgcca tcgggcgacc agcaggggcg gaaatcgtcg gcggggtcgt tggtgatgcg
     2101 taccggggcc gagccgtcgg cggccatggt gtagatctcc cagttgccgt cgcggtcgct
     2161 gtagaaggcc atccgatcgc ccgcaaacga ccagaccggc cctcggtcgt ttgcggtgtt
     2221 gctggtgatg cgcgtcaggt tggatccgtc ggcatccatc acgtagatct gagcggaccc
     2281 cgaacggtta ctgtggaagg cgatctggtc cgccgtcgtc gtgacgtgga gcagagcgga
     2341 gcccgagata cccgactcgg tatcggtggc cgtgatcgtc acggtcccgg cgccgacacc
     2401 ggtcacgacg ccattggagg ccacagtagc tacggcggcg tcgctcgtgg tccagcgcca
     2461 cgcggaggcg cccacgatga cgattgcgtt cgacgcgttc ctggctgtgg ccgtgagctg
     2521 gctcgaacca cccacaaaca cggtgggtcc gggggccgat acgctcacct gtgccaccgt
     2581 ggaaaccatg gtgaagggga catcctgtat gcccgagccg acggtgcgct ccacctcgac
     2641 ggtggcctgc atgatgccgg agccctcggc agtcggatag gccgacgcgc ggatgatgca
     2701 ttcgaggccc gcgggaagac cacccacgct gacggtcggg ttgcttcggg tggcgagagt
     2761 cgtcccgacg acgacctgcg tgcctttggt cttgatgacg aaacggacgc tctgcgaacc
     2821 cgtggggacg agtgcggctt cgacctgagg gaagtcgacg gtgaaccgga acccgccggt
     2881 gtcgaccggg gcagtggtgg tcgagcaccc gcacagggca gccaacgcgg cggccaccca
     2941 tgggagagca gcgcggcggc cggcggccgg gtgtcccagc acactaccgt cgccgcagcg
     3001 gcatggggtc gagcacaaca gacggctgga tctcactgcg gcaccccgct ggatcggacc
     3061 agcccctcag ttggaggcct ggccgttgcg gcccatgaag caaatcacga tggtctccag
     3121 catgagcgag aggtcgagcc agacattgcg ttgcttgatg tagtagagat catactcggt
     3181 cttgcggatg gattcctcca gggtggcggc gtaaccgtta cggacagtgg ccagtccggt
     3241 cacgccgggc cggacgaggt gccgcaggcg gaagtagggg atctggcgtt cgagggtctc
     3301 gacgaactcg ggccgctcgg ggcgcggtcc taccatgctc atgtggccca ggagcacgtt
     3361 ccagcattga gggagttcat cgaggtgcgt tttccggagg aggcgtccga cgggggtgat
     3421 acgcgggtcg tccttctgcg cgaactgggc gcccgtctcc tcggcattcc tcaccatagt
     3481 gcggaatttg tagatgcgga acggccttcc cttgcgcccg acacggacct gggagtagat
     3541 caccggcccc ggggacaata gcgcgttgag gccggcaatc agcgggtaca ggaccgctgc
     3601 caggaggagc ccggccgtgg ccatggcgac gtcgaccagg cgcttgacgg cggcatagat
     3661 cagggaacgt tgctggttca gtccgtagag cgcccacgcc gagtccacat agtgcacggg
     3721 gatcttgtgg aagtagcggg cgtagaagtc ctccatccag atgaccttgg tccgcgtggc
     3781 cgccagtgcc gggacgatcg ggacggcccg ggaccgaccc gtggcaggca gggcggcgat
     3841 gacgaggtcg acctggtgct gagtcgccag gttagccgca tcggcgaacc gtcccaggat
     3901 gggccagacg gcaaccgggt ggcgacccgg atcctgacgg tcatccacgt agcccaccac
     3961 ggcgaatcgg ggctcctcga cgaacggaag ggtctgggca gcctcgatgc tgtgctcgtt
     4021 aacgccgagg atgaggacgc gctcggggag taggtcgccc gagaagcgca cggcgaactg
     4081 gcgtgccagc gacgacacga tggccaacag caagaaggcg acgggcttca ccacgtggtt
     4141 ggttgatgcg cacagggtga ggacgcccag cgcgccggcc gcagcgatga acaagtcctt
     4201 gaacgtggcg tgggcactgg ccacccagcg cgactggtac gcgaggccgt tggcggccag
     4261 gaagacgaac gtgacagcgc cgagcagcca ggccggcacg cccggtatgg tcagcgctgg
     4321 caggtcgccg gggggcgcga tgacggtggc cgcccacagg atgactgagg ccgcgatgaa
     4381 gtcgtagacc atcagcgcca acgcggcacg ctgtcgtaca cgcgccaccc ggccgggtgg
     4441 gggcggtgcc accgcccgcc gttcctcgac cgcttcgggt gtctcgccta tgggcgtgac
     4501 ctcgccggcc acgccgatgt cagccattgc ggacggtcct ccgccaggaa ggtaggccac
     4561 tggcctgcgg catcctccca cggagtgcga gatccggcaa ggctagcgac gcacctcgcc
     4621 agtcccatag cactgagcgc aaagaaagga cgcccggctc cccgggggtg gcgacggaca
     4681 catgatcccg aaggacccgc cgcgcgggct tcgatgcccc cccatgccta aggtgcctca
     4741 agagcccgga gttcaccacc gcgagtggcc actggtatgc actgagggca cccctatgac
     4801 accataagcg gggccgctgg agctccgcag ctctgaccgg gacaacaggg ctcatattgg
     4861 gagttagcct tcactgatat tcggcagtga aggcagagct cccgcatccc tcctctggca
     4921 ggctcccctt gctcgaaccg ggaccatgcg gcgtaagggc ctccggtgcg tgcgggcctc
     4981 ctgggggcaa gggcagcact ttagtcccct tcagtcagtt cgacgccgcc gccaaagatg
     5041 tcaagcacca gcgcgtcggc cgagggcgcc ggggccgatg gggcagtctc accgttctcc
     5101 tgaggcacgt gctggcacga gatgcgcatg ttcnnnnnnn nnnccaagcg cgctttctga
     5161 aatgagggcg tacttttcct tgatcgctgc gcgaatgctt gggccgaact cctcgttccg
     5221 tagccgctcg ctgaacgagt cgcagccgaa cttgctgcca aatgtgatga gtagctcgtc
     5281 ttcgcccaca cgctcggcta ccgatgatgc gaggattccg cccaggcggg gcatgctctc
     5341 ggcgacccgc agcactactt tctgccagtg gtccgtcgcg cgcgccggcg ccgcggcgcg
     5401 cgcgggcgga ggcatcagca caggtgggtc atcgtcgagg ccgcacgccg gctgcgcggg
     5461 taccgcaggc gggggtggct cgggccactg ggccagcgga gcggcctccg aggatggcgc
     5521 ccgcgcgggc gccatcatgc cgaccaggct catctccagc aggaggcggt gctgatggct
     5581 ccacttcagg ttctcccgcg catcggacag ctcgcggagg cagtctacca gccgatccat
     5641 ggtgtaccct tcggcctgcc gccgcagccg cgccacggtg tcctcgctgg cctcgagggc
     5701 gctggcggag gtacatcccg acttgaccag cagcagattg cggcaatgtc cggtgagccc
     5761 gtggatgacc tgcgcgaagt ccttcccctc gtgggccagg ccgtcgacca gatggaatac
     5821 ggcggcggca tcggccgcgg ccacctggtc catgattcgg aagaacactt cgtcgtccag
     5881 ggtgcccagc accgcatgga cggcgtcgat ggtgacctgg ccctcggcgt aggcgcggat
     5941 ctggtcgaac atggtgatcg agtcacgcac gcagccgtcg gagagccgcg cgacggccca
     6001 gagcgccgcc tcgtccacgg cgatggactc ccgctcggcg atgcgggtca gatgctcgac
     6061 caattgcggc tgagcgaccc ggcggaagtc gaaccgctga caccgcgaca ggatggtggg
     6121 caacaccttc tgggggtcgg tggtggccat gatgaagtag gcgtggtcgg gtggctcctc
     6181 cagggtcttg agcagcgcgt tccacgctgc cgtggatagc atgtggacct cgtcgatgat
     6241 gtagatgcgg aaccggcccc aggaaggtgc atagccaatg acttccttca gctcgcggat
     6301 gttgtcaacg ccggtgtttg aggcggcgtc gatctcgacc acatccagca gcgaaccgtc
     6361 gcgaatcgcc tggcaggcat cgcacgtggt gcacggatca ggtgtgacgc ccttctcgca
     6421 actcagcgcc ttggccagga tgcgcgccgt tgtcgtcttc cccgtgcccc tgggaccaca
     6481 gaacaagtac gcgtgggccg gttttcctgc cgacagcgcg ttccggagcg tgcggcagat
     6541 gtggtcctgc ccaatgattt cgtcgaaggt ttgaggccgg tacttgaggt acagtgactg
     6601 atacgccatg atggctcatt atagaatgcg ctatgtcaac gcacgagccg ggaaatggac
     6661 acggagtcag ggtcgggacc cgtgtgcgtg cccaatccgc agggatgggt cctgacccgg
     6721 tttcgcgtgc cgcacggccg tgctttgcca cggttctcat cagccgccga tcggccaccc
     6781 aggacctgac gctgacttat gccgtgccgg agcgcctccg cggccggctg aggcccgggg
     6841 taccggttct ggtacccctc cggcgcgggc gcgccgtggc ccacgttctc gaggtcgatt
     6901 ccgaggcgcc gccagaaggc acccgtgccc gtgacctgat cgatgtcgtc gaggatgccc
     6961 cggacatccc ggccgaactg ctcgatctgg cccagtgggt cgccgccgag acccg
//
LOCUS       DAOSPL010000029         6903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_11040_length_6903_cov_3.504965, whole genome shotgun sequence.
ACCESSION   DAOSPL010000029 DAOSPL010000000
VERSION     DAOSPL010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6903
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11040_length_6903_cov_3.504965"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PLQ54_01020"
     CDS             <1..897
                     /locus_tag="PLQ54_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOS91870.1"
                     /translation="AFAGDTGAFIGQFPSTAPTVDRVFEGCPAVAQDGTIYVPSWDRH
                     LYALDPSGGAKWSVELSGAVRSSPVVAQDGTVYTAAGGRLYAISPAGAVLWSYDAGTE
                     IRSDPAIGLNGVIHVGCEDGHLYAVLPNGALAWSFEAQSRIRGGPSIADDGTVHFGSD
                     DRRVYALGPDGAFVWSYETGGVVEACPAVGADGAVYVGSSDGTFYVLEANGTPRWTDS
                     LPSPIYSSAALGSDGKVYVGCNNDRLYCLTSAGTVQWNSNDLWGDGHLERSSPALSSD
                     GGAFMVGRDSDEFVATLTCFRQ"
     gene            1224..2252
                     /locus_tag="PLQ54_01025"
     CDS             1224..2252
                     /locus_tag="PLQ54_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOS91871.1"
                     /translation="MRQGRSRRTLHDIAREAQVSPATVSRVLNGRLDSRIASATRDRV
                     LRIAERLGYEPNRIAQALVTGRTHMVGVVVPYRWYPFATDVMDCVQQRLEPLGWGMVV
                     EGTEGDREWFLHGVRRLEGQVDGILTVDRCDLVQALVDDPPGFGKPVVSVGSDCYRGV
                     DHVSIELGRGAADAVSHLIEQGCRRIVYVVPQISAHPHEARRLAYESTMAAAGLEPEL
                     VVFPKGTRRGAREAFLAHAAERRRLPDGLFCCNDVTALGVYRALRDLGVRIPEDVALV
                     GCDGTDETQYVDRPISTIVMPVAQMCEQGIGFLQRRIDQPDLPLQGAELTASLCIRES
                     SRRQVGDS"
     gene            2298..3071
                     /locus_tag="PLQ54_01030"
     CDS             2298..3071
                     /locus_tag="PLQ54_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOS91872.1"
                     /translation="MRCRGFTLIELLVVIAIIAILAAILFPVFAKARAKARQTSCLSN
                     LKQLANAMLQYAQDSDEGFPTHATQCSYGVTQSLQWFEQLQPYIKNTAVFQCPSAVQM
                     DEWMGNCYGGQFGRPNYIVHYGYNVQIQEAPFRNWGDNGVWSMASHQRPAEVGLLADC
                     FTTRWVSSPDPQSGISVMVAFANWTAGGAQDAFTCGCWPQITNLDLAIQKYTRHNGGS
                     NIAFADGHAKWFPAAKTILACPGYSWQGTIRVSCDGMKQ"
     gene            3120..3335
                     /locus_tag="PLQ54_01035"
     CDS             3120..3335
                     /locus_tag="PLQ54_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91873.1"
                     /translation="MWIRVMKRALLGVQVAALMVAGGCKGKQATTPEEAQAQGKAFGM
                     KQAEFMKQKMQSGTMPGTRSQGGPATQ"
     gene            3434..4018
                     /locus_tag="PLQ54_01040"
     CDS             3434..4018
                     /locus_tag="PLQ54_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOS91874.1"
                     /translation="MRAFLADYVAQLRAVLDELDPDRLQQLADRVNEARMAGQTVFII
                     GNGGSAATASHFCCDLAKGASIEGLPRIRALSLTDNTSMISAVGNDIDYDSIFSEQLD
                     VLARPGDVLIAITASGNSPNILKAVEFAKQRGLWTVGLIGFGGGKLKALVDLDITVSS
                     RNYGCVEDLHLILDHVLSQYLKHQSESSRCSQTT"
     gene            complement(3982..6399)
                     /locus_tag="PLQ54_01045"
     CDS             complement(3982..6399)
                     /locus_tag="PLQ54_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91875.1"
                     /translation="MRGVLCLVGATVVAFGGPANATPVVLFDDDAEGEISVVDSAGAT
                     KVELTRVGVDGGVAPVRGERMLRLAGTSEAPAGGGGSRAYIRFWTGHVPVTAGMRLSY
                     CVYARPESVAHGFAIDLAAPDFYPNNLRDQPAAVDQNGVRAHPSLGFLADRSIGQWCY
                     REISLEAIAGREITAAYAAWDAGGPESGPFAGYFDQVMLWTAGDTEAQALADRCRAAC
                     APGAMAEEDLSAFGVPVGLGAPAPLAPVSPTLAPPPADAGLVVLGANGYFQFSNGRPF
                     LPWGMFGSEIYTQFGRDGLTGPGPWWSHVPPAGDVGDNSEPVEISEDDWTAYFAECRR
                     RGITALRLFTYGHATPEGEPGYYFPLDAVGKVHPVLWGKLDRFMQAGYEQGILFLFTL
                     IASPDWMPYVSHPRPDSPANQVLHEARRQYAAEELTRLDAARRRFLLAERTVSIGDAF
                     RNDAFFEDADIAACVESYLRDLLPRLAADQRVFGLEIMNETFPTKYAQWARDHVVRLC
                     RELAPGRPVTVSHTLGSGVQSLEAASFVRTAGLDFYNWHSYLRQNAPVTMELASNCLF
                     YNAPVPGFPTEGPAYYGGIDPGQVDERSRRLAIRDYLWLPLVTGCPGSILWAPENSRI
                     APAEWEELGRFHAVLAEISGGIDFAALRREPPAIELAVDLPPGLRLAYTTRCLRHGTA
                     VGFGVDGSTSAAVRISEPEPLADLTTLPRPVRAVGCDLAPYLAREGYADVIAYLPNRL
                     REARGTAARPVELVLNLPPGRYRARAYAIGEQTRAEGIVDAHCRVAIAESTTDDYVVW
                     LQRLDSL"
     gene            complement(6408..>6903)
                     /locus_tag="PLQ54_01050"
     CDS             complement(6408..>6903)
                     /locus_tag="PLQ54_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91876.1"
                     /translation="DEAPYTYARVQLTTAQPLVVGERGDALVAVHRVGGGRVIVITPD
                     YWMTDALPYRNPDLVNMEPPYQILRGVREVLDGYFASFSPVRIEPAGLNVRVNCFDGD
                     DGRLLVTLMNNDLFADWTGTVHLERDIARATDIWHGAELPPGRDLQLAIPAGDVAVLD
                     LRQE"
BASE COUNT         1137 a         2284 c         2408 g         1074 t
ORIGIN      
        1 gcgttcgccg gggacaccgg ggcgttcatc ggtcagttcc cgtctacggc gccgaccgtc
       61 gatcgcgtct ttgagggctg cccggcggtg gcgcaggacg ggaccatcta cgtgccgagc
      121 tgggatcgcc acctgtatgc actcgaccct tcgggcggcg cgaagtggag tgtcgagctg
      181 agtggcgccg tacgcagcag cccggtcgtg gctcaggatg gtaccgtcta caccgccgcc
      241 ggcggccggc tgtacgcgat cagcccggct ggggcggtgc tctggagcta cgacgcggga
      301 acggagatcc gtagcgaccc ggccatcggg ctcaacgggg tcatccacgt gggctgcgag
      361 gacggtcacc tctatgccgt cctccccaat ggcgccctgg cgtggagctt cgaggcgcag
      421 agccggatcc ggggcggccc atccatcgcc gacgacggca ccgtccattt cggcagtgac
      481 gatcgccgcg tctacgctct ggggcccgac ggggcgttcg tgtggtccta cgagacgggt
      541 ggcgtggtcg aggcctgccc ggccgtgggg gccgacgggg ccgtgtacgt cggctcgagc
      601 gatggcacgt tctacgtgct ggaggccaac gggacgcctc ggtggaccga ctcacttccg
      661 agccccatct acagcagcgc cgccctgggc agtgacggca aggtctatgt ggggtgcaac
      721 aacgaccggc tctactgcct gacgtcggcc ggcacggtcc agtggaacag caacgatctg
      781 tggggcgacg gccacctgga gcggtcgagc ccggctctct catctgacgg gggcgccttc
      841 atggtcgggc gcgacagcga cgagttcgtt gcgacgctga cgtgcttccg acagtagggg
      901 gtagtggagc gcgcggaccg gggtccatgc actgacgccc gaatgggctt cctctgtcgg
      961 ccaaggcgca aggggcaccg gcccagtgcc ctcggtcgag agcaggcgcc gctcgagatt
     1021 cctcgcgccg cggatgcggc actcaggctg acaccgttgt cggtacgctc cggcggcgtg
     1081 tgggctgccg cagccagtga ccatacctac cccagctcgt gccccctgtt tcgccggcct
     1141 acttgacggc tcaggatggg cacggtacca tatgcgcaaa cgtttgcgca atgcaccgct
     1201 ccctgggggc tcggaggacg acggtgaggc aaggacgcag ccgccgcacg ctccacgaca
     1261 ttgcccggga ggcccaagtc tccccggcca cggtctcgcg ggtcctcaac ggccggctgg
     1321 acagtcgcat tgcgtccgcg acgcgcgacc gggtcctgcg catcgccgag cgtctgggct
     1381 atgagcccaa ccggatcgcg caggccctgg tcaccgggcg cacgcacatg gttggggtcg
     1441 tcgttccata ccgctggtac cccttcgcca cagatgtcat ggactgcgtc cagcagcggc
     1501 tcgaacctct tgggtggggc atggtggtcg aaggcactga aggcgaccgc gagtggttcc
     1561 tccatggcgt gcgccggctc gagggacagg tcgacggcat tctcaccgtc gatcgttgcg
     1621 acctggtgca ggccctggtc gatgacccgc ccgggtttgg caagccggtg gtgagtgtcg
     1681 gctcggactg ctaccgcgga gtggatcacg tgagcatcga gctgggacgc ggggcggcgg
     1741 acgcggtgag tcacctcatt gagcagggct gccgccgcat cgtgtacgtc gtgccccaga
     1801 tctccgctca cccgcacgag gcacgacggt tggcctatga gtccaccatg gcagctgccg
     1861 ggctcgagcc ggagctcgtc gtcttcccca aaggcacccg ccggggtgct cgagaggcgt
     1921 ttctggccca tgcggccgaa cgccgacgac tgcccgatgg tctcttctgc tgcaatgacg
     1981 tgacggcctt gggcgtgtac cgagcgctgc gcgacctggg cgttcgcatt cccgaagacg
     2041 tggcccttgt cggatgcgat ggcaccgatg agacgcagta cgtcgaccgc cccatcagca
     2101 ccatcgtgat gccggtcgcc cagatgtgcg aacagggcat tgggttcctg cagcgccgca
     2161 tcgatcagcc ggacctcccg ctccagggcg cggagctgac ggcatctttg tgtatccggg
     2221 agtcgtcccg acggcaagtc ggagactcct gagcaacgcg gggccgacgg ggctccgcca
     2281 ggaagggact ggttcatgtg cgatgcaggg ggttcacgct catcgagctt ctcgtggtga
     2341 tcgccatcat cgcgatcctg gccgccatcc tgttccccgt cttcgccaag gcccgggcca
     2401 aagcccgcca gacttcgtgc ctctccaacc tcaagcaact cgccaacgcc atgctacagt
     2461 acgcccagga cagtgatgag ggcttcccga cgcacgccac ccagtgctcc tatggcgtga
     2521 cgcagtcgtt gcagtggttc gagcagctcc agccctatat caagaacacc gctgtgttcc
     2581 agtgcccgag cgccgtccag atggacgagt ggatgggcaa ctgctacggc gggcagttcg
     2641 gacgtccgaa ctacattgtc cactacggct acaatgtgca gatccaggag gcgcccttcc
     2701 gcaactgggg cgacaacggc gtgtggagca tggccagcca tcagcgcccc gccgaagtgg
     2761 gccttctggc cgactgcttc accacccggt gggtctcatc ccccgacccg cagtccggca
     2821 tatccgtcat ggtcgccttc gcgaactgga cggctggagg ggcgcaagat gcgttcacgt
     2881 gcggttgctg gccgcagatc accaacctgg acctcgccat ccagaagtac acgcggcaca
     2941 acggcgggtc gaacatcgcg ttcgctgacg ggcacgccaa gtggttcccc gcggccaaga
     3001 cgatcctggc gtgccccggg tatagctggc agggaaccat ccgtgtgtcc tgtgacggca
     3061 tgaagcagta gcggcggggg cgcggtcggc cccgtcccat gcgaagtgga gaggtcacca
     3121 tgtggatccg tgtgatgaag cgggcgttgc tgggcgtcca ggtcgccgcg ctgatggttg
     3181 ccgggggatg caaaggcaag caggcgacca cacccgagga agcccaggcc cagggcaaag
     3241 cgttcgggat gaagcaggcc gagttcatga agcagaagat gcagagtgga accatgcccg
     3301 gcacgcgctc ccagggcggc cctgccacac agtaggctgc ctcttgcaca ccagcgcctt
     3361 tcgggtcatg ctgccggcgg tgccgtccat cgcggcgccg ccggcagtcc acacgacccg
     3421 gagggacatg tgcatgagag cattcctggc cgactacgtg gcgcagctcc gcgccgtgct
     3481 cgacgaactc gaccccgacc gcctacagca gctcgccgat cgcgtgaacg aggcgcgaat
     3541 ggccggccag acggtgttca tcatcggcaa cggcggcagt gcggccacgg cctcacactt
     3601 ctgctgcgac ctcgccaagg gcgcctcgat cgagggcctg ccgaggatcc gcgccctgtc
     3661 gctcaccgac aacacgtcga tgatcagtgc tgtggggaat gacatcgact acgacagcat
     3721 cttctccgag cagctggacg tactggcgcg acccggcgac gtcctgattg ccatcacggc
     3781 ttccggcaac tcgcccaaca tcctgaaggc cgttgagttc gctaagcagc gcgggctgtg
     3841 gaccgtgggg ctcatcggct tcggcggtgg caagctgaag gcgctcgtcg acctcgacat
     3901 caccgtgtcc agccgcaact acggatgtgt ggaggacctg cacctcatcc tcgaccacgt
     3961 gctatcccag tatctgaagc atcagagcga gtcgagtcgt tgcagccaga cgacgtagtc
     4021 gtcggtggtt gactccgcga tcgccacgcg gcagtgggcg tctacgatgc cctcagcgcg
     4081 agtctgctcg ccgatggcgt aggctcgggc tcggtagcgg ccgggcggga ggttcaggac
     4141 cagctcgacg ggtctcgcgg ccgtgccacg ggcctccctg agccggttcg gcaggtatgc
     4201 gattacgtcg gcgtagccct cacgggccag gtagggcgcc aggtcgcaac cgactgcgcg
     4261 gaccggacgg ggtagggtgg tgaggtctgc cagaggctct ggctccgaga tcctgacggc
     4321 ggcgctcgtg ctcccatcga cgccgaagcc cacggccgtg ccgtgccgca ggcaacgggt
     4381 cgtgtaggcc aggcgcaggc ccggcggaag gtcgaccgcc agctcgatgg cgggcggctc
     4441 gcggcgaagc gcggcgaagt cgatgccacc cgagatctcg gccagcacgg catggaaccg
     4501 ccccagctcc tcccactcgg ctggcgcgat gcggctgttc tccggcgccc acaggatgga
     4561 cccgggacaa ccggtcacca gagggagcca gaggtaatcg cgaatggcga gccggcggga
     4621 tcgttcgtcc acctgccccg ggtcgatgcc gccgtagtag gccgggccct cggtggggaa
     4681 cccggggacc ggcgcgttgt agaagaggca gttcgaggcc aactccatcg tcacgggggc
     4741 gttctggcga aggtaggagt gccagttgta gaagtccagc ccggcggtcc gaacgaagga
     4801 ggcagcctcg agactctgga cgcccgagcc gagcgtgtgg ctcaccgtca cgggccggcc
     4861 gggggccaac tcccggcaga gccggacgac atggtcccgc gcccactggg catacttcgt
     4921 cgggaatgtc tcgttcatga tctcgagacc gaacacgcgc tggtccgcgg cgagccgcgg
     4981 caacaagtcc cgcaggtagc tctcgacaca cgccgcgatg tcggcatcct cgaagaaggc
     5041 atcgttgcgg aacgcgtcgc caatgctgac ggtgcgctca gccagcagga accggcggcg
     5101 cgcggcatcg aggcgtgtga gctcctcggc agcgtactgc cgccgggcct cgtgcagtac
     5161 ctggttcgcg ggcgagtccg gccggggatg gctgacatag ggcatccaat cgggtgaggc
     5221 gatcagggtg aacaggaaca agatgccctg ctcgtagccg gcctgcatga accggtcgag
     5281 tttgccccac agcacggggt ggaccttgcc cacggcatcc agcgggaagt agtagccggg
     5341 ctcgccctcg ggcgtggcgt gcccgtaggt gaacagtcgc agggccgtga tgcctcgccg
     5401 tcggcactcg gcgaagtagg cggtccagtc gtcctcggag atctccactg gctcggagtt
     5461 gtcccccaca tcccccgccg gcggcacgtg tgaccaccag gggccggggc cggtgagccc
     5521 atcgcggccg aactgagtgt agatctcgct gccgaacatg ccccagggca ggaacggccg
     5581 cccgttgctg aactggaagt agccgttcgc gcccagcacg acgagaccgg catcggccgg
     5641 cggaggcgca agggtggggg agacgggggc gagaggggcg ggcgccccga ggcccacggg
     5701 gactccgaag gcggacagat cctcctcggc catcgccccg ggcgcgcacg ctgcccggca
     5761 ccgatcggcc agggcctgcg cttcggtgtc gccagccgtc cacagcatca cctggtcgaa
     5821 gtatcccgca aacggcccgg actcggggcc gccggcgtcc catgcggcgt aggcggcagt
     5881 gatctcgcgg ccggcgatcg cctcgaggct gatctcgcgg tagcaccact ggccgatgct
     5941 gcggtcggcc aggaagccaa gcgacgggtg cgcgcggacg ccgttctggt ccacagcagc
     6001 aggctggtcg cgcaggttgt tcgggtagaa gtcgggcgcg gccaagtcga tggcgaaccc
     6061 atgggccacg gactcgggcc gagcgtagac gcagtagctc agccgcatgc cagcggtgac
     6121 gggcacgtgc ccggtccaga accggatgta ggctcgggac ccgccaccac cggcgggtgc
     6181 ctcggatgtg ccggcgaggc gaagcatgcg ctcgccccgt acgggtgcaa cgccgccatc
     6241 gacgcccacg cgggtcagct cgaccttggt ggcaccggcg gagtccacga ccgagatctc
     6301 accctcggca tcgtcatcga acagcaccac gggtgtggcg ttcgctggcc caccgaaggc
     6361 aaccacggtc gcgccaacca ggcagagcac accacgcatg ggttcctcta ctcctgacgc
     6421 aggtcgagca cggcgacgtc gcctgcaggg atcgcgagct gcagatcgcg cccggggggc
     6481 aactcggccc cgtgccagat atcggtggcc cgggcgatgt cccgctcgag gtgtactgtg
     6541 ccggtccagt cggcgaagag gtcgttgttc atcaacgtga cgagaagccg cccatcgtcg
     6601 ccgtcgaagc agttcacccg gacgttgagg cccgcgggct cgatccggac agggctgaac
     6661 gaggcgaagt agccatcgag gacctcccgg acgccccgca ggatctgata gggaggctcc
     6721 atgttcacca ggtcggggtt gcggtagggc agggcatcag tcatccagta gtcgggggtg
     6781 atgacgatca cgcggccacc gcccacacgg tggacggcca ccagggcgtc gccgcgttcg
     6841 ccgaccacca ggggctgggc cgtggtgagc tgcactcgtg cataggtgta gggcgcctcg
     6901 tcg
//
LOCUS       DAOSPL010000030         6844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_11155_length_6844_cov_3.254087, whole genome shotgun sequence.
ACCESSION   DAOSPL010000030 DAOSPL010000000
VERSION     DAOSPL010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11155_length_6844_cov_3.254087"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..985)
                     /locus_tag="PLQ54_01055"
     CDS             complement(221..985)
                     /locus_tag="PLQ54_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91877.1"
                     /translation="MTFERGAALVGVLAVAVLVGYGSYRVRMSLEAKPSPEAALAEAP
                     ARPQSDTGTTGFPVVRAPRNLFQPLRLQAPRSKEQAAVSAPPTSPPVSGGWYPPYTMP
                     YIPPAYGGGFVPASGLPGSSGAQAPPGEPSPAGPEPEGPRPPQPDAAEIAVTAVSTGP
                     SGPRVLVENLQTHRSQWVSSGGTAFGYTLERATGKGGLFRKGGRYYALGLGDGKSGNP
                     VPSASQPTAPPAEGAPTPGAPAVRPAPSPPPAAATP"
     gene            complement(1059..1616)
                     /locus_tag="PLQ54_01060"
     CDS             complement(1059..1616)
                     /locus_tag="PLQ54_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HOS91878.1"
                     /translation="MNTRERNLVNVAAMVAVFVLAFLFLLDPMLKARREARDDIRKAE
                     RRLESLEKNGLGSSEGRELARLRGMVGVGREGQAETQSTRILRSVENACIQAQAIPLN
                     IVPLDSSEEAGLIRHPVEVQAKGTLQAVFQMLANLSEVRPAVGIERLNMEYDEKTDLL
                     TVQLVVASFTPKTQTTAEGKPSAKS"
     gene            complement(1621..3231)
                     /gene="pilM"
                     /locus_tag="PLQ54_01065"
     CDS             complement(1621..3231)
                     /gene="pilM"
                     /locus_tag="PLQ54_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOS91879.1"
                     /translation="MGRAKSSMGVDLGSQSIKVVQIRRVGRDYRIDHAAIIPVRRSAD
                     DPYSVEQPDLLLRGHVATAGLRPGATVCSVDRGQSTVRSVRLPVSRPADLDQMVRYEA
                     ETLVPFPIDSVQLGYASSGAHGDLADVVIAACPVESLQGRMGFFASAGVAPTELAVSS
                     LALYNCLMAAMPERRMATQLVVDLGARCTEVLVVAEGALVTSLSLRHGGDGLTESLAE
                     DLKMDPHEAENHKRTHEIPFDPETGLPPADMPATRAWLTRLFEQLRRVIDAHHQDGTK
                     PRIESVVLCGQGAEMPGITAALGAGLQLPVTVLDPLASLPIDDHAVTARHGPAFANAV
                     GLALQGLGEAALQVNLRPADVSARGPVSMSRVWVLAWVAFLTLLAVTCATVNSGLQRD
                     KERLERLENRIARLDLTGRGDESASLADLEQYRQIAARVQDPSADWFGLFGEMCDVLP
                     PDLSLRTWTCERGKEVTLRGRALSNAAVADAVDALRGLDRFTSVRPTHSSAVKVSGRT
                     VYDFEIRAELPKPEKSKTANTREANRST"
     gene            complement(3235..>3826)
                     /locus_tag="PLQ54_01070"
     CDS             complement(3235..>3826)
                     /locus_tag="PLQ54_01070"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOS91880.1"
                     /translation="RQVPSDDMPTGAGSIPSDLDMPDMQTGLPSELLQFGGEQPAPVT
                     PAPTTGREAEQEQAFASLADLLSVEGITTDDLSRIADRVTTTDEGIRYGLVNINTAPV
                     EVLAAVAGLNENLAEEIVRWRQSQGAFANIAQLLEIDGINQEVFSEIAGALTVRSYVF
                     TIEALGQVPQSGIVKLIRCTVDVSGDQPNILRYAER"
     assembly_gap    3827..3836
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3837..4669)
                     /locus_tag="PLQ54_01075"
     CDS             complement(<3837..4669)
                     /locus_tag="PLQ54_01075"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOS91881.1"
                     /translation="MRARQNRHSRGVALAVTLWVLAILGMVAATYALTVQTNTHLSAN
                     FSDELVALGLARAGLEITLARLIEGHHQYTGAETGWDDRTSEEDEIDFDRGTYRVRIF
                     DEGGKLDVNRATEEQWGLLLDDERLAAAVMDWIDEDDETRPDGCESFYYQGLTPSYSA
                     RNGAVTSVEELGLVRGMTLEKLWGDAGLLNVPRRSGEVRGLVDLLTVTSIDSNLDSEG
                     EKRVNINTADQQALSEALGEYLSEEQIQAIIAYRDEHGRRSAPTGGGSGAPVSPTVPG
                     LP"
     gene            complement(4672..5250)
                     /locus_tag="PLQ54_01080"
                     /pseudo
     CDS             complement(4672..5250)
                     /locus_tag="PLQ54_01080"
                     /inference="COORDINATES: protein motif:HMM:NF023044.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
     gene            complement(5717..6307)
                     /locus_tag="PLQ54_01085"
     CDS             complement(5717..6307)
                     /locus_tag="PLQ54_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS91882.1"
                     /translation="MAARRRDSAGFTLLELVVATAILAIGLVALTQVFSRGLRSTGSS
                     ERVVTATLIARLKLAEMDEMTELTNGAESGDVAEPYKGYTWDTEIEDVPQGKNLKRVR
                     VRIGWTEAGLPRDVSLETVLFKPPAEEEAGQEEEEEAGGQTGALGAAGTGDFPAGGMG
                     TGTGMGGGGIPSGLGDFFSSVGGRTNTPVGRRGVSR"
     gene            complement(6311..>6844)
                     /locus_tag="PLQ54_01090"
     CDS             complement(6311..>6844)
                     /locus_tag="PLQ54_01090"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HOS91883.1"
                     /translation="RNLVSACRVARSRAVTRARPVRVMLDAHQHVYSLAELVDPDEEA
                     ERRGQPLGPGGGSVTPEPTREWIPVQSHMGRGRKLPTGITFSYVGGRPDQEDTERRSA
                     RDEELGSTRNPGTDWVEFYPDGTAEDTYIELTNTADNALWVHVDPITGVPQFLSKEEI
                     SRLLATTSSGAATGASQ"
BASE COUNT         1022 a         2436 c         2215 g         1161 t
ORIGIN      
        1 caacggtagc gccagcagca cgagcacggc gagggccgag cgggcgtgcc cgcgcgcccg
       61 cctcggccgg ccggccgagg ccctcgcgcc gggaccggag tggagcacca gcggtggggc
      121 tgggctatgg gttctgtctg ttcggctcac gtctgactcc ctgcgtcttc tggcctagac
      181 tcgcccctgg caggccccgt ccgtggggcc gtgagttcgg tcatggtgtc gcggccgccg
      241 gcggtgggct gggtgccgga cggactgccg gcgctccagg cgtgggcgcg ccctcggccg
      301 gcggtgccgt cggctgactg gccgacggca ccgggttgcc ggatttgccg tcgcccaacc
      361 cgagcgcata gtagcgaccg cccttgcgga acagcccacc cttgcccgtg gcacgctcca
      421 gcgtgtagcc aaatgcggta ccgcctgagc tgacccactg cgaacggtgt gtctgcaggt
      481 tctccactag cacccgagga ccagaaggcc ccgtcgacac ggctgtcacc gcgatctcgg
      541 ccgcgtccgg ctgcggaggt ctgggccctt ccggctcggg tccggccgga ctgggctcac
      601 ccggaggcgc ctgcgcacca ctggaccccg gtagccctga ggctggcacg aacccgcccc
      661 cgtaggccgg cgggatgtac ggcatcgtat agggtgggta ccatccgcct gagaccgggg
      721 gactggtcgg aggcgcggag accgccgcct gctccttgct ccgcggggcc tggagccgca
      781 gaggctggaa caggttgcgg ggcgcccgga cgaccggaaa cccggtggtc cccgtatcgg
      841 actggggccg ggccggcgcc tcggcgagcg cggcctcagg cgacggcttg gcctccaaag
      901 acatgcggac acggtaggac ccgtagccta ccagtaccgc gacggccagc acccccacca
      961 gggccgcgcc tcgttcgaag gtcatacgcg ccccccttct cgctcgcagt ccctggcaga
     1021 tgctgcgggc gctcttctca tgcgcggccg cggagatctc acgacttggc ggacggcttc
     1081 ccctcggccg tggtctgcgt tttgggcgta aaggaggcga ccaccagctg cacggtcagc
     1141 agatccgtct tctcgtcgta ctccatgttc agccgctcga tacccaccgc ggggcgcact
     1201 tcagacaggt tggccaacat ctggaacacg gcctgcagtg tccccttggc ctggacctcg
     1261 accggatggc ggatcagacc cgcttcctcg ctgctgtcga gtggcacgat gttgaggggt
     1321 atggcctggg cctggatgca ggcgttctcg accgagcgca ggatccgagt gctctgcgtc
     1381 tccgcctgcc cctcccgtcc cacgccaacc atgccccgca ggcgggccag ctcgcggccc
     1441 tccgacgaac ccaggccatt cttctccaga ctctccagcc tgcgctcggc cttgcggatg
     1501 tcatctcgcg cctcgcgccg tgctttgagc atcgggtcca gcaggaacag gaaggcgagc
     1561 acgaacaccg cgaccatggc ggcgacgttg acgaggttcc tctcgcgggt gttcatgggc
     1621 tcacgtactc ctgttcgctt ctctggtgtt cgccgtcttg gacttctcgg gcttgggaag
     1681 ctccgcgcgg atttcgaagt catagacggt gcggccagag accttgacgg cgctcgagtg
     1741 tgtggggcgg accgacgtga aacgatccag tccccgcagc gcatccacgg catcggccac
     1801 tgcggcattt gagagcgcgc gtccccgcag cgtgacctcc ttgccgcgct cacatgtcca
     1861 ggtgcgcagg cttaggtcgg gtggcaggac atcacacatc tcgccgaaca gcccaaacca
     1921 gtcggccgac ggatcctgta cccgggcggc gatctgccgg tactgctcca gatcggccaa
     1981 cgatgcgctc tcgtcaccgc gtcccgtcag gtccagccgc gcgatgcggt tttccagacg
     2041 ctcgagcctc tccttgtcgc gttggaggcc cgagttcacc gtcgcgcatg tcacggccag
     2101 cagagtgaga aaggccaccc acgctagcac ccacacgcgg gacatcgaca ccggaccccg
     2161 agctgacacg tccgccgggc gcaggttcac ctgcagcgcc gcctcgccca gcccctgcag
     2221 cgcgagtcct acggcgttgg cgaaggcggg tccgtgccgc gccgttacgg catggtcatc
     2281 gatgggcagc gacgcgagcg gatcgagaac cgtgaccggc agctgcagtc cggcgcccag
     2341 tgcggcagtg atgcccggca tctccgcgcc ctggccgcag agcacgaccg actcgatgcg
     2401 cggcttggtt ccgtcctggt ggtgtgcgtc gatcacgcgc cggagctgct cgaacagccg
     2461 agtcagccag gcacgcgtgg cgggcatgtc cgcgggcggg aggcccgtct ccgggtcaaa
     2521 cggtatctca tgcgtccgtt tgtggttctc cgcctcgtgc ggatccatct tcaggtcttc
     2581 cgccaggctt tcggtcagcc cgtcgcctcc atgccggagc gagagcgatg tgaccaaagc
     2641 gccctcggcc accaccagaa cctcggtgca gcgcgcgccg aggtcgacca ccaactgcgt
     2701 cgccatcctc cgttcgggca tggcggccat gaggcagttg tagagggcca ggctcgacac
     2761 cgccagctcg gtgggcgcca cccccgcgct cgcgaagaag cccatccggc cctggaggct
     2821 ctccaccgga catgccgcaa tcacgacatc ggccaggtcg ccatgcgccc cggatgaggc
     2881 atacccaagc tgcaccgagt cgatcggaaa cggcaccaag gtttcggcct catagcggac
     2941 catctggtcc aggtcggctg gtctggacac cggaagccga accgaccgga cggtggactg
     3001 gcctcgatcg acactgcaga ccgtggcacc tggtctcagt ccggctgtgg ccacgtgacc
     3061 gcggagcagc aggtctggct gttcgacgct ataagggtcg tcggcggagc ggcgcaccgg
     3121 gatgatggcg gcgtggtcga tgcggtaatc acgccccacg cgccgtatct ggaccacctt
     3181 gatggactgg gagcccagat cgacccccat gctcgacttg gctctgccca cggttcaccg
     3241 ctccgcgtaa cgcagtatgt tcggctggtc ccccgatacg tcgacagtac agcggatcag
     3301 cttcacgatg cccgactggg gcacctggcc cagcgcttcg atggtgaaga cgtacgatcg
     3361 gactgtcaac gcgccggcga tctcagagaa gacctcttgg ttgatgccat cgatctcgag
     3421 gagctgggcg atgttcgcga aagcgccctg gctctgacgc catcgcacga tctcttcagc
     3481 caggttctcg ttcaatccgg ccactgccgc caagacctcg acgggtgccg tgttgatgtt
     3541 caccagccca tatcggattc cctcatcggt cgtggtcacc cggtctgcaa tgcggctcag
     3601 gtcatccgtg gtgatcccct cgacactgag aaggtctgcc agtgacgcga aggcttgctc
     3661 ctgctcggcc tcgcgcccgg tggtgggcgc cggtgtaacc ggcgccggct gctccccgcc
     3721 gaactggagt agctcagacg gcagtccggt ttgcatgtca ggcatgtcaa ggtcggaggg
     3781 tatcgacccc gcgccagtgg gcatgtcatc cgagggaacc tgtcgtnnnn nnnnnnggca
     3841 agcccgggac ggtgggactg acgggcgcgc cgctgccacc gcccgtgggc gcgctgcgtc
     3901 tgccgtgctc gtcgcggtac gcgatgatgg cctggatctg ctcctcagac aggtactcgc
     3961 ccagggcttc cgagagtgcc tgctggtccg ctgtgttgat gttgacgcgc ttttcgccct
     4021 cggagtccag gttgctgtcg atgctcgtca cggtcaggag gtcaaccagt ccgcggacct
     4081 cgcccgagcg gcggggaacg ttgagcagtc ccgcatcacc ccagagcttc tcgagggtca
     4141 tcccgcggac aagccccagc tcttcgacgc tggtcaccgc gccgtttcgg gccgagtacg
     4201 acggcgtcag gccctggtag tagaacgact cgcagccatc cgggcgcgtc tcgtcgtctt
     4261 cgtcgatcca gtccatgacg gcggcggcga gccgctcatc gtcgaggagc agcccccact
     4321 gctcctctgt ggctcggttc acgtccagct ttccgccctc gtcgaagatc ctcacacggt
     4381 aggtcccgcg gtcgaagtct atctcgtcct cttcggaagt gcggtcatcc catccggtct
     4441 cggcgcccgt gtactggtgg tggccctcga tgagccgtgc cagcgtgatc tccaggcccg
     4501 cccgtgccag gccgagagcc accagctcgt ccgagaagtt ggccgacagg tgcgtgttcg
     4561 tctgcaccgt cagcgcgtag gtggccgcga ccatgcccaa gatggccaga acccacaggg
     4621 tcacggccag cgcaacccct cggctgtggc ggttctggcg agctcgcatg gctacctcgc
     4681 cccctcaaag agatcggacg ggacgcctcc gccagtgccc gtgccgcctc ctacgccgcc
     4741 ggcaccgccg ccgtcgaaga accccgccgg cggaccaccg tccacgccgc ccatgaagcc
     4801 cgccccggca tcggggaaga agccacttgg tggggcgccg cccgtgggct cgccggtcgt
     4861 cgtggaggtg ctcggctccg actctctccc ggccagagcc tcgcccggcg ccttcacgcc
     4921 cacggtcttc aggttcacca cggccgaaac gatcacggga tcctcaaccc cggccgcatc
     4981 cgccaccgag agcgtgacct cgacggacac gggtgccgtc tcgtcctccc actcatccgt
     5041 ccactcctcg ttggtggagt cgtagtagcg gaagctgaga cttcgcacca gaggcgagag
     5101 ctgttcggtg atccccgatc gggtcgagtc ctcagccaga tcgaggtacc ggtcctcgga
     5161 ccgcatgagt ccctgctcgg gcgttgcgtc gtcgaggtca aggtagtagc ggactcgcac
     5221 gatgaccgtg ggcggctgcg tcctctgcgc ctgggtcctg ccaccgtgtc aaactcgatc
     5281 tcgtcggcct cctccaggcc gggtggcccg ttggtaccca ccaggggagg tgtcgcctcc
     5341 tcggccggct tctcagcctc gccggacgtg gcaggaaccg cgaagtcatc ctcgccagca
     5401 gcccgtgcgt ccgagggccc cgccgtggtc gccgcctccg cgccggcttc cgcggctccc
     5461 tcggtgctcg tgctggacac gctctgggcc tgctgggctt cgggctcgat gtagatggag
     5521 gacagctcgg cggcgatctg gtcgatgagg gcccgtgcgg tctgggtcat ctcgccccgg
     5581 cgatccacaa tctgagtggc gttgcggccg gtccgaaagg tggcgtacac gccgcccatc
     5641 acgaccacca gcaagatcga gacgaccatc acctcgatca gactgaagcc cgagcgcgca
     5701 cgacgacgaa cgggggtcat cggctcaccc ccctgcggcc cacgggggtg ttggtgcggc
     5761 cgccgaccga gctgaagaag tcgccgagcc ctgagggaat gccgccgcca cccatgcccg
     5821 tccccgtgcc catgccgccg gccgggaagt cgcccgtgcc cgcggcgccc aacgcacccg
     5881 tctgcccccc ggcttcctcc tcctcctcct gtcccgcttc ctcctcggca gggggcttga
     5941 acaacaccgt ctcgaggctc acgtccctgg gcagcccggc ctcggtccag ccgatgcgaa
     6001 cgcgcacacg tttgaggttc ttgccctgcg gaacgtcctc gatctcggtg tcccaggtgt
     6061 agcccttgta gggctccgcc acatcgcccg actcggcccc attggtcagt tcggtcatct
     6121 cgtccatctc ggcgagcttc agccgagcga tcaacgtggc cgtgaccacc cgctccgacg
     6181 aaccggtcga gcgtaaccca cgactgaaca cctgggtcag ggccaccagg ccgatcgcca
     6241 ggatggccgt cgccacaacc agttccagca gggtaaagcc tgcactatca cggcgccgcg
     6301 cagccatggg ctattgagac gccccggtcg ctgcgccact tgaggtggtg gccaacaacc
     6361 gggagatctc ctccttgctc aggaactggg gaacgccagt gatcgggtcc acatgcaccc
     6421 acagggcgtt gtccgcagtg ttggtgagct caatgtacgt gtcctcggcg gtgccgtcgg
     6481 ggtagaactc cacccagtcg gtccccgggt tccgcgtcga cccgagctcc tcatcgcgcg
     6541 ccgaccggcg ctcggtgtcc tcctggtcgg ggcgtccgcc gacatacgag aacgtgatac
     6601 cagtgggaag cttgcgccct cgccccatgt ggctttgcac cgggatccac tcccgcgtgg
     6661 gctcgggagt cacgctcccg ccaccgggcc cgagtggctg tcctctccgt tcggcctctt
     6721 cgtcagggtc gacgagctcg gccaacgagt agacgtgctg gtgggcatcc agcatcaccc
     6781 ggaccggtcg ggctcgagtc acggcgcggc tccgcgccac gcggcatgcc gacaccagat
     6841 tccg
//
LOCUS       DAOSPL010000031         6821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_11212_length_6821_cov_3.047295, whole genome shotgun sequence.
ACCESSION   DAOSPL010000031 DAOSPL010000000
VERSION     DAOSPL010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11212_length_6821_cov_3.047295"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..1280)
                     /gene="larA"
                     /locus_tag="PLQ54_01095"
     CDS             complement(6..1280)
                     /gene="larA"
                     /locus_tag="PLQ54_01095"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOS91884.1"
                     /translation="MRYGRGYASVEVPDGRLVSVLRGAAAPALADAHAAVQHAIGSPI
                     GGEDLAAMAVGRRHAVVVVSDITRPAPNALMLPPVLDALRRGGIDHHRVTVLIATGAH
                     RRTSPQELGELLGPDLPAACRIADHVATDHEAHTYLGTTPQDVPLHMDRRYVDADLKV
                     LTGLIEPHYMAGFSGGRKAVCPGLASVETIGRFHRHELLMHERSTTGVLAGNPVHEAA
                     LAATRAAGADFIVNAALDDRRQVTGVWAGELEAAHLAGCHEVARSASAACPEPVDIVV
                     TSNAGYPLDCSVYQATKGMVGALEILKPGGTVVIAAECSEGLGSPAFASVLRADSRIE
                     DSLARLADPSSTAMDQWGVTMLAKAVTRARVLIYADGLSEADARSAYLTRVPSLQSGL
                     DQALRWHGPEATVAVIPDGPYTMATLAPDGLG"
     gene            1462..4398
                     /locus_tag="PLQ54_01100"
     CDS             1462..4398
                     /locus_tag="PLQ54_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOS91885.1"
                     /translation="MAEKLLANPPLCWDSPRGLQLLPEWAEEHPELQRAAARFQALAG
                     STLPVAMQGLDRNGVVHLWSESSARLYGMPAEAAMGRRLQELLLPEDQWDRFEALLAA
                     IWSVQRGEPPCEWPVAARSGPLWVLTTMFPVVEGGRVVEVIRMDVDVTARKQAETELA
                     HERFLLHALMDNIPDAIYFKDVESRFVRINAAQARTLGASSPEEAVGRTDMDYQVPEI
                     AAESLRDERHVLGTGRPLVAKAEPVRTHDGRAKWVSTTKVPLRDSGGTITGLVGISRD
                     ITELRVAEEQLRSRVEEDRRLLDRLRALHQVSNRLSLCASVDDMCREAVTAATSVLGF
                     ERVGIWLWDRESQTLVGTFGTDEDGRVRDERGTRRELHEPSDTRMALEAHEPWVRALD
                     RELHDATGTVVGRGSWGAAPMWDGQDTIGVVCTDTLFSSRALQDTDCEVLALLAAAIG
                     HHTTRLRVQERLQESEQRHRVLAEQSFLGICIVQDDRVSFANRAFADLAGCSPEALRT
                     LTVSDLVAMVHPDDRESVARQYRRKTSGDPCAAPHFTYRIVRDGRVRWVEQYSQAIML
                     SGRPADFLTTADITDRVEAEQERRRLETRLQDAEKNESLAVLAGGVAHDFNNLLVGVL
                     GHASLALISLDADAAVRPYIEQIEAAATRAAELANQMLAYSGRGRFQLQPIDLSELVS
                     DMMPLLEASVSHKCVLRGDLACGLPPVDGDPTQLRQIVLNLVKNASDAIDRGSGLVTI
                     ETGTRSVAAGELTEDLLGDGLPTTPCVFLRVTDTGHGMDEATQRRVFEPFFTTKFTGR
                     GLGLAAVLGIVRGHGGAIGLTSRPGEGTTFEVLLPASRREPREASPAPTTAPALAEPA
                     ATGCVLVVDDEEDVRLVARAALEFAGFTVLTANNGREGLAIFREQSDRICAVLLDLTM
                     PEMGGAETLPELRRVRPDARVVISTGYGEDETLERLGHVPPTGFIQKPYGAPDLIAKI
                     RSVIAP"
     gene            complement(4522..5649)
                     /gene="ispG"
                     /locus_tag="PLQ54_01105"
                     /gene_synonym="gcpE"
     CDS             complement(4522..5649)
                     /gene="ispG"
                     /locus_tag="PLQ54_01105"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOS91886.1"
                     /translation="MPLPPADPAVGALPEPRRPTRGVNVGRVRVGDGAPIAVQSMCKT
                     DTRDVDATLRQIRDAHSAGAEIMRVAVPDEAAARALRRIVAQSPVPIVGDIHFNHRLA
                     LAAVDAGVAKIRINPGNIGSPEKARAVVDRAGEAGIPIRVGVNSGSLERDLLDSSGHV
                     TARALVESALRHVDRLESWGFRDIVVSLKASDVWRTIQACRLFSAASDVPQHIGVTEA
                     GLVLEGTAKSAAALAVILGMGIGDTVRVSLTGDPVDEVRVAWAILSALDLRRRGPIVI
                     SCPTCGRTEINLRDLAERVADAVRDCRIPITIAVMGCVVNGPGEAREADVGICGGRGS
                     GIVFRRGEVVRKVPESELLDALLDEVRALEAEHGGVSQAPT"
     gene            complement(5630..>6821)
                     /locus_tag="PLQ54_01110"
     CDS             complement(5630..>6821)
                     /locus_tag="PLQ54_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOS91887.1"
                     /translation="YPILPQAQRQRSLGIGPAIRQWQRGDTTYSLRMIPFAAYVKIVG
                     MEPGEEYEEGSIYTRPLFGRLATIVAGAMMNIVLAFLFLTAAGVIFGLGERESDRIAM
                     VAKDSPAAAAGVKEGDRVITLDGATPSGGVPGMIAYLARRPGKPVRVGLLRGGQRMEL
                     VMTPVETAVAPDEARKLGLPAGTTKRGMLGITFGSESYRTDASPIDYVVAGATDTWVQ
                     IVGTVAFVKRLVTRTTEKGDGLVGPIGVVRVVSAVVKRGLQPLLYLFAVLNVSIGLLN
                     LFPIPMLDGGRFVIMLVEGLRQRFFGRGLFDKQKEAAVHAVGLILILMFVVTVSAGDI
                     RRLARREDPFESAIQEERAKAQQELDRRLKATPQGAKGQTSGPLTGTRARGEHGAPDA
                     AASR"
BASE COUNT         1126 a         2314 c         2357 g         1024 t
ORIGIN      
        1 tcgcgtcagc ccagcccgtc cggcgcgagg gtcgccatgg tgtaggggcc atcgggaatc
       61 accgcgaccg tggcctcggg tccatgccac cttagggctt gatcgaggcc actctggagc
      121 gacggcaccc gtgtaaggta ggccgaccgt gcatccgctt cggacagacc gtcggcgtag
      181 atcagcactc gggcacgagt cacggccttg gcgagcatgg tgacacccca ctggtccatg
      241 gcggtactgg acgggtcggc gaggcgcgcc agggagtcct cgatgcgaga gtcggcccgg
      301 agtactgagg cgaaagccgg gctccccagt ccttcagagc actcggccgc aatcaccacg
      361 gtgcccccgg gcttgaggat ctccaaagcc cccaccatgc ctttggtcgc ctggtagacc
      421 gagcagtcga gcgggtagcc cgcgttggat gtgacgacga tgtccacggg ctcggggcac
      481 gcggccgaag cgcttcgggc gacctcatgg cacccggcaa ggtgggcggc ctcgagctcg
      541 cccgcccaca cgcccgtgac ctggcggcgg tcgtcgagcg cggcgttgac gatgaagtcg
      601 gcgcccgcgg cccgggtggc cgccagtgcc gcttcgtgca ccgggttccc ggccagcaca
      661 ccggtcgtgg atctctcgtg catgagcaac tcatgccgat ggaaacggcc gatggtctcc
      721 actgaggcga ggcccgggca gacggccttg cggccgccgg agaagccggc catgtaatga
      781 ggctcaatga gacccgtgag caccttcaga tccgcgtcca cataccggcg gtccatgtgc
      841 agcggcacat cctgaggggt ggtacccagg tacgtgtgag cctcgtggtc cgtggcgaca
      901 tggtccgcta tgcggcaggc ggcgggcaag tcgggcccca ggagctcacc aagctcctgc
      961 ggcgaggtgc gccggtgggc tccagtggcg atgaggaccg tgacgcggtg gtggtcgatg
     1021 ccgccccgcc gaagcgcatc gaggaccggg ggcagcatga gcgcgttggg tgccgggcgc
     1081 gtgatgtccg agaccaccac aaccgcatgg cgtcggccca cggccatggc ggcgagatcc
     1141 tcacccccga tcggtgaccc gatggcatgt tggaccgccg catgggcatc cgccagtgcc
     1201 ggcgccgcag ccccccgcaa caccgagacg aggcgcccgt cgggcacctc gacggacgca
     1261 taaccccgcc cataccgcac accggtcaac accgtggacc tgtcctctca tccacacggc
     1321 ggagatcggc cagccctgat ggagaacgat gctccgcacg gagggcctct tgtggaacga
     1381 tgcccaatgg agacaacacc ccggtccgtg cacggtgccc ggggggctag ccgcggagcg
     1441 cggctcaaaa cggggggagc catggcggag aagctactgg cgaacccgcc gctgtgttgg
     1501 gattcgcctc gggggctaca gctcctcccg gagtgggccg aggagcaccc ggagctccag
     1561 cgtgccgccg cgcgctttca ggcgctggcg ggaagcaccc ttcccgtggc catgcagggc
     1621 ctggaccgca acggcgtcgt tcacctctgg agcgagtcgt cggcgcgtct atacggcatg
     1681 ccggccgaag cagccatggg gcggcgcttg caggagcttc tgctgcctga ggaccagtgg
     1741 gaccgcttcg aagcactgct ggccgccatc tggtcggtcc agagggggga acctccgtgc
     1801 gagtggccgg tggccgcgcg gtcggggccg ctctgggtac tgaccaccat gttcccggtg
     1861 gtggaaggtg gccgggtcgt cgaggtcatc cgtatggacg tcgacgtgac cgcgcgcaag
     1921 caggcggaga cggagctggc tcacgagcgc ttccttctcc acgcactcat ggacaacatc
     1981 cccgacgcca tctacttcaa ggacgtcgag tcccgcttcg tgcgcatcaa cgcagcgcag
     2041 gcacggacgc tgggagcctc ttcgcccgag gaggccgttg ggcgcacgga catggactac
     2101 caggtgcccg agatcgcggc cgagagccta cgggacgagc ggcacgtgct cggtacgggg
     2161 cgaccactgg tcgcgaaggc cgagccggta cggacgcacg acggccgggc caaatgggtg
     2221 tccaccacca aagtgcccct ccgcgacagt ggtggcacca tcacgggcct ggtgggaatc
     2281 agccgggaca tcactgagct acgggtggcc gaggagcaac tgcggagccg ggtcgaagag
     2341 gaccggcggc tcctcgaccg gctccgagcc cttcaccagg tgtcgaaccg gctctcgctg
     2401 tgcgcgtcgg tcgacgacat gtgtcgcgag gccgtcacag cggccacctc ggtgctgggc
     2461 ttcgagcgcg ttggcatctg gctgtgggat cgtgagagcc agacgcttgt gggcacgttc
     2521 gggaccgacg aggatgggcg cgttcgcgat gagcgcggca cccggcgcga gctgcatgag
     2581 ccgagcgaca cgcggatggc gctcgaggca cacgaacctt gggtccgtgc cctggatcgc
     2641 gaactgcacg atgccacggg cacggtcgtg ggccgtggat cgtggggcgc ggcgcccatg
     2701 tgggacggcc aagacaccat cggcgtggtc tgcacggaca cgctgttctc gtcacgcgcc
     2761 ctgcaggata ccgactgcga ggtgctggca ctgctggccg ccgccatcgg ccaccacacc
     2821 acccgactgc gcgtgcagga gcggctccag gagagtgaac agcgccaccg ggtactggcc
     2881 gagcagtcat tccttggcat ctgtatcgtc caggacgacc gtgtgtcgtt cgccaaccgg
     2941 gctttcgccg atctggcggg ctgctcgccg gaggcgctgc ggacgcttac ggtctctgat
     3001 ctcgtcgcca tggtccaccc cgacgaccgc gagtccgtgg cccgccagta ccggcggaag
     3061 acgtccggtg atccctgtgc cgctccccac ttcacctatc gcatcgtgcg cgatggccgc
     3121 gtgcgatggg tcgagcagta ttcgcaagcg atcatgctca gcggccgccc ggcggacttc
     3181 ctcacaacgg ccgacatcac cgaccgggtc gaggccgaac aggagcggcg gcggctcgag
     3241 acgcgcttgc aggacgccga gaagaacgag agcctggccg ttctcgcggg cggagtcgct
     3301 cacgatttca acaacctgct ggtcggcgtt ctgggtcacg cgtctctggc gctcatcagc
     3361 ctcgacgccg atgcggcggt ccggccgtac atcgaacaga tcgaggcggc cgcaacgcgg
     3421 gcggcggagc tggccaacca gatgctggcc tattcgggcc gcggacgctt ccagctccag
     3481 cccatagacc tctccgagct cgtctctgac atgatgccgc tgctggaagc ctccgtgtcg
     3541 cacaagtgtg tgctgcgtgg cgacctcgcg tgcggcctcc cgcccgtgga cggcgacccg
     3601 acgcagctcc ggcagatcgt gctcaacctg gtgaagaacg catccgacgc catcgatcgg
     3661 ggctcgggac ttgtgaccat cgagaccgga acccggtcag tagccgccgg cgagctcacg
     3721 gaagacctgc tgggcgatgg gttgccaacg accccgtgcg tgttcctgag agtgaccgac
     3781 acgggccatg ggatggatga ggcgacccag cggcgcgtct tcgagccctt cttcacgacc
     3841 aagtttaccg ggcgcggctt gggactcgcg gccgtgctgg gcattgtccg aggacacggg
     3901 ggcgcgatcg gcctgaccag ccgacccggg gaggggacca ccttcgaggt gctcctgccg
     3961 gcctcaaggc gcgagccgcg ggaggcgagt ccggccccca cgacggctcc cgccctggct
     4021 gaaccggcgg cgaccggatg cgtgctcgtc gtcgatgacg aggaggatgt ccgtctggtg
     4081 gcgcgggctg ccctcgagtt tgccggcttc accgtgctca cagccaacaa cggccgcgag
     4141 ggactggcca tcttccgtga gcaatccgac cggatctgcg ccgtgctact ggacctgacc
     4201 atgcccgaga tgggaggcgc ggagacgctc cccgagttgc gccgcgtgcg acccgatgct
     4261 cgcgtggtca tctcgaccgg ctatggtgaa gatgagacgc tggagcgcct cggtcatgtg
     4321 ccgcccaccg ggttcatcca gaagccctat ggcgcccccg acctgatcgc caagattcgt
     4381 tccgtcatcg cgccgtagag ccggggccac agccgcctgg ctgccggcgt gcgaatccag
     4441 agcggttccg ggaatcgtgg tgacggcgca gcccatggtc gggtggcgca tgcctcacgc
     4501 tcgcgtgggt cgcgcgagag gctacgtggg tgcttggctc actcccccat gctccgcctc
     4561 gagcgcgcgg acctcatcga ggagggcatc gagcagctcg gactcgggga ccttgcgaac
     4621 gacctcgccg cggcggaaca cgattccact accgcggccg ccacagatac cgacatcggc
     4681 ctcgcgggct tcgcccggac cgttcacgac gcaccccatc actgcgatcg tgatggggat
     4741 tcggcagtcg cgaacggcat cggccacacg ctcagccagg tcgcgcaggt tgatctcggt
     4801 ccgcccacag gtcgggcagg agatgacgat cggacctcga cggcgcaggt ccagggccga
     4861 cagaatcgcc caggcgacgc gcacctcgtc gacgggatcc ccggtcagtg agacccggac
     4921 ggtgtcgccg atccccatac ccaggatcac cgccagcgcg gccgccgact tcgcggtgcc
     4981 ctcgaggacc agacccgctt cggtcacccc aatgtgctgg ggaacatcag atgccgccga
     5041 gaacagacga caagcctgga tggtccgcca cacgtccgag gctttgagcg agaccacaat
     5101 gtcgcggaaa ccccacgact ccagacggtc cacgtgccgg agtgcgctct cgaccagggc
     5161 ccgcgccgtg acgtggccgc tggagtcgag cagatcgcgt tcgagcgacc cgctgttgac
     5221 gccgacacgg atcggaatgc ccgcttcgcc ggctcggtcc acgacagccc tggccttctc
     5281 gggcgagccg atgttgcccg ggttgatgcg gatcttcgcg acaccggcgt cgacggcagc
     5341 cagcgccagc cggtggttga agtggatatc gcccacaatc ggcacggggg actgggccac
     5401 gattcgccgg agagcccgcg ccgccgcctc gtcgggcacg gcgacacgca tgatctcggc
     5461 accggccgaa tgagcgtcac ggatctgccg gagcgtggcg tcgacgtcgc gggtgtcggt
     5521 cttgcacatg gactggaccg cgatgggagc cccatcgccc acgcgcaccc gaccgacatt
     5581 cacgcctcgg gtcggccggc ggggctcagg aagcgcgccc acggccggat cagcgggagg
     5641 cagcggcatc gggcgctccg tgttcgccgc gggcccgggt gccggtcagc ggcccgctcg
     5701 tctgcccctt ggcaccctgg ggagtcgcct tcagccgccg gtcgagttcc tgctgcgcct
     5761 tggctcgctc ctcctgaatc gccgactcga atgggtcctc acggcgcgcc agccgccgaa
     5821 tgtcgcccgc agagacggtg accacgaaca tcaggatcag gatcaggccg acggcgtgga
     5881 cggcggcctc cttctgctta tcgaacagcc cccgcccgaa gaaccgctgg cggaggccct
     5941 caaccagcat gatgacaaac cggccaccat cgagcatggg gatggggaac aggttcagca
     6001 ggccaatact cacattgagt accgcgaaca gatagagcag cggctgaagg ccgcgcttca
     6061 cgaccgcgga aacgactcgg actacgccga tggggcccac caggccatcg cccttctcgg
     6121 tcgtgcgtgt caccaagcgc ttgacgaagg cgacggtgcc cacgatctgc acccaggtgt
     6181 ccgtggcgcc cgcgaccaca tagtcgatcg gcgaggcgtc ggtgcggtat gactcggacc
     6241 cgaaggtgat accgagcatg ccgcgtttgg tggtgcccgc cgggaggccg agcttccggg
     6301 cctcgtccgg ggcgacggcg gtctcgacgg gagtcatcac cagttccatc cgctgaccgc
     6361 cccgaagcag gcccacgcgc acgggcttgc cggggcgccg agcgagatag gcgatcatgc
     6421 cggggacgcc acccgatgga gttgcgccat ctagcgtgat gacgcgatcg ccttccttga
     6481 ctccagccgc ggccgccggc gagtccttgg ccaccatggc aatccggtcg gattcgcgct
     6541 cccccaggcc gaagatcacg ccggcggcgg tgaggaacag gaaggccaga acgatgttca
     6601 tcatcgcgcc cgccacaatg gtcgctagcc ggccgaagag aggccgcgtg tagatactgc
     6661 cctcttcata ctcctcgccg ggctccatgc cgacgatctt gacgtaggca gcgaagggga
     6721 tcatccgaag ggagtacgtg gtgtctcccc gctgccattg tcggatggcc ggaccgatgc
     6781 cgagggagcg ctgacgctgc gcttggggaa ggatgggata t
//
LOCUS       DAOSPL010000032         6815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_11225_length_6815_cov_3.707249, whole genome shotgun sequence.
ACCESSION   DAOSPL010000032 DAOSPL010000000
VERSION     DAOSPL010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11225_length_6815_cov_3.707249"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..1680
                     /locus_tag="PLQ54_01115"
     CDS             274..1680
                     /locus_tag="PLQ54_01115"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOS91888.1"
                     /translation="MGLIVGALGHLAVVLPLLSLIAAMSGCGSGQNPPPPGVGLGTPI
                     RTAIAIGPAGSPEPNLTSLIAGPRGLVFGCTEENGWIVILDPITGRSMQWNAGHTSPT
                     PTSGAYEGDITDLTAWEESGRIVIIACQAYPAAIYRYSFLVDASATGSAALPEPGDIS
                     GECVYENPEWARIHSVARSAASGLLLAGSHHGEGAIVASRDGGFTWEILDYMSTVPGL
                     NVDGLKAVEHSAWSVSGLHWIDGTWFACLFNSFVLEAEIGLSGTQRHYANAVFYSRDE
                     GASWALSTVTRTSPLPDTVQAAVDQDRAKGRLPEPFYGLINERMQSLVRGTNGRLYAT
                     TTFDYTTDLSGGGRVYASADGGTTWTETARLEGFTDANIIALPNGLLVAGTMTKLEEL
                     PTSQAAATADLWVSDDNGQSFSHFATVASTTSKSTIHPGFYVIGVASMAYASGHLVVG
                     TVNESAGGSAEILLVPVT"
     gene            1739..3193
                     /locus_tag="PLQ54_01120"
     CDS             1739..3193
                     /locus_tag="PLQ54_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91889.1"
                     /translation="MLEDGRAYAMGAAAIMVLLIVAQRRLIFSPSVLGVYCTATLIFT
                     VVGVLVMPFVRPHLRAIFPRLLLQGLTGRDFLYAYIIVVGGLAVVLASYQAAHAISHG
                     GRLCRQGPRLVGRVKGSVLDFSLSALVVWASLSALVTATMLFQYRSSIRLGVVEGFIA
                     ASPGAVFTVRRSIDSNYPLILMTRTILPFYCVAAWLAWRVTGWRWVRRFAIGMIAMSL
                     VMLFSIFAKGALVAYLLMLAGLGAWGYSRRGFPRSGRQTEGFSLPTRKLALYFTGAFV
                     VLCLFYYVSTPAARHAGKGPLSAVGTVLSISLTRLFGRLALEMPMYTHLFPDVHEHYG
                     LTNVGLLNRLWGAEFFPDTFYVFSAFSDSPIAGSAAVPALGDFYGQFGFLGWFIGACV
                     LGVLLQCADSILVTVRDRPSGVLLVVAGMTFAYYLTQANLPRAALGYGGVLYLLQWLS
                     LAPGSLRAMWVRPRRPPAYRLSAARGGASSRPPA"
     gene            complement(3105..4679)
                     /locus_tag="PLQ54_01125"
     CDS             complement(3105..4679)
                     /locus_tag="PLQ54_01125"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOS91890.1"
                     /translation="MRDPLPMVLQAKDTAAQPQNGSRTAFRAGLLVYYATVMFSTAAM
                     MFAIRQADHLLSAEALGLLMIARRLSGTGSSLAQLGVSQTLIRYVGLYAKNPAAKRRY
                     VVFAAAVWGLLTAIILPAAFLFQPMLASLCFPGASAGPTMVVWSVALMLSMVGILIAH
                     STLLAEQRMVWAGVVTGMEVGGFFLGLLVVLGSRSTPLNLIAGQAVGATVLSAAVVAW
                     YCQSPWRARGGDAPPWRDVRSDFITYGLPRGVISGLDMSVLIIGPWLLRADPKQAGSL
                     LIALTMLRVLNALLGPITKLTSALTARYVGSGDETSIERAVKMLFGMAFIASVLFTAG
                     LVPWRDQLLRLWLAKPETIAGTSAHFSVLCWSVLPLSIFYALRGAIEMRWKTPYNLYT
                     LLTAIAVHLVVYYACARTMAQPVAVRVAITACMWVMGILTVIWARDYLKPVAYWGLPQ
                     VAAVGTALYALSTWADQLGGIEGVSILAAAIAGGAAILVRYAPCPFMLEAWNWLRHGQ
                     PIAGTPVGDAAGPTSL"
     gene            4903..6042
                     /locus_tag="PLQ54_01130"
     CDS             4903..6042
                     /locus_tag="PLQ54_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91891.1"
                     /translation="MDFLTDGRGYSVGAALVVVVIMLAQWRVILSASLIGVFCTGTLV
                     FTIVGLLLMPWMRPYLPTWFPRLQLEAIGGQDYLYADLLMVGGLALVLMAYQASHALA
                     HHGRLCRTGPRLTALMRTSRVDFSLPALLVATLAALAASVALMLQYRSQIEVGLVEGF
                     LGANPLAMFVARREVGENYALFQLVQNVLPYYAVAMWLVWRVTGHRPIRGAALVACTA
                     AAVMLVFLFQKRPLLVFLLMLGLLSAWGPSWRRALERRRRSVRPRASAVRVVLFGSLL
                     FALLCVLYFFSTTVVRGAPGGLVGAVGTLGQIALTRVVGRLALPVPMYTHVFPGIHEH
                     YGLSNVGLLGRLWGAEFFPDTFYVFSVFSNSGISGSAAVPALGDF"
     gene            6091..6363
                     /locus_tag="PLQ54_01135"
     CDS             6091..6363
                     /locus_tag="PLQ54_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91892.1"
                     /translation="MALQWGDSVLVRVRDRPSGVLLIMAAMIFAYYLTQANLPRALLG
                     YGGVVFLAQWLLLAPACLRRTWRRTRPVAQTGVASAAEGATPASRR"
     gene            complement(6323..>6815)
                     /locus_tag="PLQ54_01140"
     CDS             complement(6323..>6815)
                     /locus_tag="PLQ54_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91893.1"
                     /translation="WLTKDETIQGTWVHFSVLCWGVLPLTVFYALRGVIEVRWKTPYN
                     LYTLAAAIGVHIVGYYAFARYMPPGPAVRASLMASLWVMGLLTMVWTRQYLRPVSYWG
                     LPQLALIGAAIFAVNLWGAGVRGAAGPAASAAAAASGVAVLVRSARCPFIADLRVWLL
                     RRR"
BASE COUNT         1040 a         2195 c         2318 g         1262 t
ORIGIN      
        1 gagatgaagc catagccctt ctcattgttg aaccacttga ctttgccgcg cacgagccct
       61 gcactcctcg tgtcagaata ggttggaggg agggttccgg ctctcacatt cggtaccctc
      121 cgtggacgca aaaaccgact gcaggccctc ccgcctgtga gtctgcggga aggccccgtg
      181 cgtgacccgc cgaagtgaac atgatgccgg cggccgagga ctcccggaac gggcccgccc
      241 cggggcgaga ccatctcgtt gtggagggaa ctcgtgggac tgatcgtggg cgcactgggt
      301 catctggcgg tggtcctgcc gctcctgagc ctcattgcgg caatgagtgg ttgcggctcg
      361 gggcagaacc cgccccctcc cggcgtcgga ctggggacgc cgatccgcac cgccatcgcg
      421 attggccccg ccggctcacc agaaccgaac ctgacgtcgc tcatcgcggg acctcggggt
      481 ctcgtgttcg gctgcaccga ggagaacggc tggatcgtca tcctcgatcc gatcacgggc
      541 agatccatgc agtggaacgc gggtcatacg tcgccgacgc cgacctccgg tgcctatgag
      601 ggtgacatca ccgatctgac ggcctgggag gagtccgggc gcatcgtcat catcgcctgc
      661 caggcgtacc ccgccgcgat ctaccggtac tcgttcctgg tggacgcctc cgctacgggc
      721 agcgcggcgc tgccggagcc cggggacatc agcggcgagt gcgtctatga gaaccccgag
      781 tgggcccgga tccactcggt agcccgctcc gcggcgtccg gcctgctcct ggcagggtcg
      841 caccacggcg agggggccat cgtcgccagt cgtgacggag ggttcacctg ggagattctg
      901 gactatatgt ccaccgtgcc gggcctgaat gtggacgggc tgaaggcggt ggagcacagc
      961 gcgtggtcgg tctcgggcct gcactggatc gatggcacct ggttcgcatg cctcttcaac
     1021 tcgttcgtgc tcgaggcgga gatcgggctg agcggcaccc agcggcacta cgccaatgcc
     1081 gtgttctact cgcgtgatga gggcgcgtcg tgggcgctga gcacggtaac acgcacgtcg
     1141 ccgttgccgg acaccgtgca ggcggccgtg gatcaggacc gagcgaaggg ccggctgcct
     1201 gagccgttct atgggctcat caacgagcga atgcagagcc tggtgcgcgg aacgaacggt
     1261 cggctgtacg ccaccaccac gttcgactac accaccgacc tctcgggcgg cgggcgtgtc
     1321 tacgcctcgg ccgacggtgg cacgacgtgg actgagacgg cacggctcga ggggttcact
     1381 gacgcgaaca tcattgcgct gccgaatggg cttctcgtgg cgggcacgat gacgaagctc
     1441 gaggagctgc ccaccagtca ggccgcggcc acggccgacc tatgggtctc cgacgacaac
     1501 ggccaatcct tcagtcactt cgccaccgtg gcgagtacga catcgaagtc gacgatccac
     1561 ccgggcttct acgtgattgg tgtcgcgtct atggcctacg cgtcgggcca cctggtggtg
     1621 gggaccgtga acgagtcggc tggcgggagc gccgagatcc ttctggtgcc ggtgacctga
     1681 ggtcggttgc gcgcgctcgc gaaccattcc cgatggctga ggaggccagg gccgctcgat
     1741 gctcgaggac ggtagagctt acgcgatggg tgccgccgcc atcatggtgc tccttatcgt
     1801 ggcccagcgg cgcctgatct tctcgcccag cgtcctcgga gtgtactgca cggcgacgtt
     1861 gatcttcacc gttgtcggcg tgttggtcat gcccttcgtc cggcctcacc tgcgcgccat
     1921 attcccccgg ctgcttctgc agggcctgac gggccgcgac ttcctctatg cctacatcat
     1981 tgtcgtgggc ggattggccg ttgtgttagc gagctaccag gccgcgcacg ccatctcgca
     2041 tggggggcga ttgtgccgcc agggcccacg gctggtgggg cgggtgaagg ggagtgtcct
     2101 ggacttctcg ttgtcggctt tggtggtttg ggcctccctc agcgccttgg tgacggcaac
     2161 gatgctcttc cagtaccggt cgagcatcag gctgggtgtt gtggaggggt tcatcgccgc
     2221 cagtcccggc gccgtgttca ccgttcggcg gtccatcgac agcaactatc cgctgatcct
     2281 gatgacccgg accatcttgc cgttctactg tgtcgccgcg tggttggcgt ggcgggtgac
     2341 gggctggcga tgggttcggc ggttcgccat cggcatgatc gccatgtctc tcgtgatgct
     2401 gttctcgatc ttcgccaagg gggcgttggt cgcgtacttg ctgatgctgg ccgggctggg
     2461 cgcatggggt tactcgcggc ggggattccc gcgctcgggc cgccagaccg aggggttctc
     2521 cctgcccacc cgcaagttgg cgctgtactt cacaggggcg ttcgtcgtgc tgtgcctgtt
     2581 ctactatgtc tcgacgcccg cggcccggca cgcgggcaag ggacctctgt cggcggtggg
     2641 gacggtgctg agcatctcac tgacgcgcct cttcggacgc ttggcccttg agatgcccat
     2701 gtacactcat ttgttcccgg acgtgcacga gcactatggg ctgacaaacg tcgggctgct
     2761 gaaccggctc tggggagccg aattcttccc ggacactttc tacgtgttta gcgcattctc
     2821 ggattcgccc atcgccggct cggccgccgt ccctgccctg ggtgacttct acggccagtt
     2881 cggcttcctt gggtggttca ttggggcctg tgtgctgggg gtcctattgc agtgcgctga
     2941 ctcgattctg gtgaccgtcc gggaccgtcc cagcggcgtg ctgctggtgg tggccggcat
     3001 gaccttcgcg tactatctga cccaggccaa cctgcctcga gccgcgctgg gctacggcgg
     3061 cgtgctgtac ctcttgcagt ggttgtccct cgccccgggg tcgctcagag cgatgtgggt
     3121 ccggccgcgt cgcccaccgg cgtaccggct atcggctgcc cgtggcggag ccagttccag
     3181 gcctccagca tgaaggggca cggcgcgtag cgaaccaaga tcgccgcccc accggcgatg
     3241 gcagcggcca ggatgctcac gccttcaatg ccgccgagtt ggtcggccca tgtactcagc
     3301 gcatagaggg ccgtgccgac ggccgcaacc tgaggaaggc cccagtaggc caccggcttc
     3361 aagtagtccc gcgcccagat cacggtcagg atccccatca cccacatgca cgcggtgatg
     3421 gccacgcgca cggcgacggg ctgcgccatg gtccgcgcgc atgcgtagta gaccaccaag
     3481 tgcacggcga tggccgtgag cagtgtgtag aggttatagg gcgtcttcca gcgcatctcg
     3541 atcgccccgc ggagggcgta gaagatcgac agaggcagca cactccagca gagaaccgag
     3601 aagtgggcgc tggttccggc gatggtctcg ggcttcgcga gccacagcct gagcagctga
     3661 tcccgccagg gcaccaggcc ggcggtgaac aggactgagg cgatgaacgc cattccgaac
     3721 agcatcttca ccgcccgctc gatggacgtc tcgtcacccg accccacgta ccgtgccgtg
     3781 agcgccgacg tgagcttggt gatggggccc aagagggcat tgagcactct cagcatcgtg
     3841 agggcgatga gtaggctgcc ggcctgcttc gggtcggccc tgaggagcca cgggccgatg
     3901 atcaggacgc tcatgtcgag gcccgagatc acgccccggg gtaggccata ggtgatgaaa
     3961 tcgctgcgga cgtcgcgcca cggcggggca tcccctcccc gggctcgcca tgggctctgg
     4021 cagtaccagg ctaccaccgc ggcactcaga accgtggcgc cgaccgcctg tccagcgatc
     4081 aggttgaggg gtgtggagcg gctcccgagc accaccagca gtccgaggaa gaacccgccc
     4141 acctccattc cggtcacgac gcccgcccag accatccgct gctcggccag gagtgtggag
     4201 tgtgcgatga ggatgccgac catgctgagc atgagcgcca cagaccacac gaccatggtg
     4261 ggcccggccg atgctccggg gaagcataga ctcgccagca tgggctggaa caggaatgcg
     4321 gccggcagga tgatggccgt gaggagcccc cacacggccg cggcgaagac gacgtagcgg
     4381 cgcttggccg ctgggttctt ggcgtagagt ccgacgtagc ggatcagtgt ctgcgacacg
     4441 ccgagctggg cgaggctcga accggttcca ctgaggcgcc gggcgatcat cagcagcccc
     4501 aacgcctcag ccgacagcag atggtccgcc tggcggatgg cgaacatcat tgccgctgtg
     4561 ctgaacatca cggtcgcgta gtagacgagc agtccggccc ggaacgcggt ccgcgacccg
     4621 ttctgaggct gggcggccgt gtctttggcc tgcaggacca tggggagagg atcacgcatg
     4681 gtgcactgat aataccttcc catctgaact cgcgtccgag catgaggcga ctcggcgtct
     4741 ggcagacgga tgcccaccca gctcagtgtg cgaccgacgt gccgattcct gcggggtcat
     4801 ctgagtgccc gccgatgctc ggtgggccac ggcctcgacc cgcgccggag gccatggtat
     4861 acgtgggagc cgaaggacgt catctgcggg gggaggtgaa aagtggactt cctaaccgat
     4921 ggcagaggct actccgtcgg cgcagcactc gttgtcgttg tcatcatgtt ggcgcagtgg
     4981 cgggtcatcc tctcggcaag cctgatcggg gtcttctgta cgggcaccct tgtcttcacc
     5041 atcgtggggc tgctgctcat gccgtggatg cgcccctacc tacctacctg gttcccgcgg
     5101 ctccagctcg aggcgatcgg gggacaggac tatctgtacg ccgacctcct gatggtgggc
     5161 ggactggcat tggtcttgat ggcctatcag gcatcacacg cgctcgcgca ccatggacgt
     5221 ctctgccgca ccgggccacg gctcacggca ctgatgagga cctcccgtgt ggatttctcg
     5281 ctcccggcgc tcctcgtggc aacgctggca gcgctggccg cgagcgtggc gctcatgctg
     5341 cagtaccgtt cgcagatcga agttggtctt gtcgagggct tcctgggggc gaacccgctc
     5401 gccatgttcg tggcgcggcg cgaggtgggc gagaactacg cgttgttcca gctcgtgcag
     5461 aacgtgctcc cgtactatgc cgtcgccatg tggctggtgt ggcgcgtgac cggtcatcga
     5521 ccgatccgcg gggccgccct tgtcgcatgc acggcggccg ccgtgatgct cgtgttcctg
     5581 ttccagaagc ggcccctgct ggtgttcctg ctgatgctcg ggcttctgag tgcctggggt
     5641 ccatcgtggc ggcgcgcgtt ggagcgtcgg cggaggtcgg tgcggcctcg ggcctccgca
     5701 gtacgagtcg tgttgttcgg gtcgctgctg ttcgcattgc tgtgcgttct ttactttttt
     5761 agtactactg tagtccgtgg agcccccggc gggctggtgg gtgcagtcgg cacgctcgga
     5821 cagatcgcgc tgacccgcgt tgtgggccgg ctggccttgc cggtgccgat gtacacccat
     5881 gtcttcccgg ggatccatga acactacggg ctgagcaatg tggggttgct cggccgcctc
     5941 tggggagccg agttcttccc tgacaccttc tatgtgttca gcgtcttctc gaacagcggc
     6001 atctcgggct cggcggcggt gccagcgctg ggcgacttct agggccagtt cggtttcacc
     6061 ggatggttcg tcggcgcttg ccttctcggc gtggcgctgc agtggggcga ctcggtcctg
     6121 gtgcgggtgc gcgatcgtcc cagtggcgta ctactgatca tggcggcgat gatcttcgcc
     6181 tactacctga cgcaggcgaa cctaccgagg gcgttgctcg gctacggcgg ggtggtgttc
     6241 ctcgcccagt ggctgctcct ggccccggca tgcctgcgcc ggacatggcg ccgcactcgc
     6301 ccggtggccc aaacgggcgt tgctagcgcc gccgaaggag ccacacccgc aagtcggcga
     6361 tgaacgggca cctggcggac cgaacgagga ccgccacgcc cgaagcagct gctgccgcgg
     6421 aggccgctgg tccggccgcc ccccgaacac ccgcgcccca caggttgacg gcgaagatgg
     6481 ccgcgccgat gagcgccagc tgcggcagcc cccagtatga gacgggtctc aggtactgcc
     6541 gcgtccacac catggtgagc agccccatga cccacaggct ggccatgagc gaggcccgga
     6601 cggcaggccc cggtggcatg tagcgcgcga aagcatagta gcccacgata tggacaccga
     6661 tggcggcggc cagcgtgtac aggttgtagg gcgtcttcca tcgcacctcg atgacgccgc
     6721 gcagagcgta gaacaccgtg agcgggagta cgccccagca cagcaccgag aagtgcaccc
     6781 aggtaccctg gatcgtctcg tctttggtga gccac
//
LOCUS       DAOSPL010000033         6771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_11323_length_6771_cov_3.841126, whole genome shotgun sequence.
ACCESSION   DAOSPL010000033 DAOSPL010000000
VERSION     DAOSPL010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11323_length_6771_cov_3.841126"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3123
                     /locus_tag="PLQ54_01145"
     CDS             <1..3123
                     /locus_tag="PLQ54_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91894.1"
                     /translation="YDFLFDLTGLVASGETVSLAFRYLNPDIPRALGVERAPLALDRI
                     ALDSMPTADVERRRREALKGQRVTRAPVEPRLPSGAKRGERPYEIEWSGRQESPRAQV
                     AFDDLRGWTLHVLGDASVSLSASVEHVLWRPQLARFSYAGGSIDTTVEIRPPRPIPIR
                     GRFDAANLWLYGAWDRVGDARLRITAVLEDQAGREYEMDLGAVTAAYWGMQHGVLRPS
                     VIRAARFPMKFVALRIANCRVKGERHAYLESLAFYKQDREPPRAPARPEPAVFPVSDD
                     GMLPTPPPGTVARVRPRGRGAEFSTEVRGDRLQFRIRPEEGCLHGVTARWNNGPRWHP
                     MADGGPMFDRRGVPPGTARVVSSALRHGVLTVHWREGVGRRALEWEADYSVRGCTLVV
                     DVRCSGGKATGLALGDVAGLSDPRGIEVPYLLMGQKPGPWIAHADGIFVSVLPDWYHS
                     DCSAIDTSVVPTRGDRIGLMRGTSYQPLTDGRRNPLRDRILVTVSSEFADTLPNARNP
                     VSPNRERLAPYMFYMADQLNPNLYRTLKRCGIDKVIATDFAALLVANHYAEGFAARWR
                     PHPSLTFEQVQEYRSGIKSLGYLFGTYMDVTDFYPGNEFWDEDWAVLGSDGDLTDGWW
                     GNYLVKPALMPGLIRAVGEKAKGLYPSDCVYLDVHTNRGPQALDFEAGAPGAGIAREQ
                     VVANGDCIVEAREQYGSTMSEGLYRWMYAGLTDMDYATLITTGTASELAPLVDFDLLK
                     IHPFQHGTMMGHHPACLLSSEEQAPLYADTGQGLGPESFYKYVSASLAYGHMLILGYW
                     YTPPLSRFIHYDALMQGVQREYLTDTAAEIGYHNGKALVPTSQALAEGSQQLGRVYVR
                     YSRGLRVWVNYNAEKPWAVEASGRTYELPPYGWLIEKPDEILAYSALIGGHRVDFVRC
                     PDYLYLNTGDTRAAEGPLDVDGAVWLKREGGGWRLIPCGDLGPWERFPPPGLPSNFSD
                     FRLSGVPPDRGCRSIVVDTRALLARPAGEVRITARGEAGDRTDADVRQLDDARLQFVS
                     MAGIVDCVME"
     gene            3282..4481
                     /locus_tag="PLQ54_01150"
     CDS             3282..4481
                     /locus_tag="PLQ54_01150"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOS91895.1"
                     /translation="MATTRPDVDPPDSPCSVGWTVPQTIRLATREDPFPLEVGGSIGP
                     VDVEYETYGELNKARDNVILVTHALSGDAHVAGWDKTAEATGRLWRARKPGWWDTMVG
                     PGKPLDTGRYFVVCANFLGSCYGTTGPSSPNPSTGKPYGLSFPVVTVEDWVRQQARLL
                     DALGVARIRAVIGGSLGGQQAIEWALAYPERLDKCIVLAASPRLSTQGLAFNAVGRQT
                     IMSDPNFNGGCYYDGPAPADGLAAARMLAHITYLSEPGMHDKFGRRLQDREQPAFSFG
                     IEFAVESYLDHQGKAFVERFDANSYLYITRAMDYYDAAAQWGGGDLVEACRRVQSDVM
                     VVSFSSDWLYPPAHCREFATALCQLGKPVTYVDVPSRYGHDAFLVEYDVVGSLVRSFV
                     NNERRSS"
     gene            4478..5239
                     /gene="metW"
                     /locus_tag="PLQ54_01155"
     CDS             4478..5239
                     /gene="metW"
                     /locus_tag="PLQ54_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOS91896.1"
                     /translation="MNENPPILSLGDASELAALIRDKVDPALMGLSAGKLTEYLNHVG
                     SSQPRTPGPDAGRLQRWQDDIIESTVPQGCSILDLGCGHGDLLVRLMDRKQVRGQGIE
                     LDPDAVIQCVRQGVPVVQADLDEGLKDFADESFDYAVLEQTLQTVRRPIALLQEMLRV
                     ARRGIVSFPNFAYWRVRLDLGLRGRMPITERLPYRWYDTPNIHSFTLQDFEDWTRTCG
                     VRILSGHVLAEGQVRELRASDNLYAEEVLVFVERE"
     gene            complement(5263..6555)
                     /gene="galK"
                     /locus_tag="PLQ54_01160"
     CDS             complement(5263..6555)
                     /gene="galK"
                     /locus_tag="PLQ54_01160"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOS91897.1"
                     /translation="MEGAVGLRPIHRRAAVTLPCAASYNGRARDAHSLRLRVSVDMAV
                     VDRLRGALGSMPGGPQGFRYFRAPGRVNLIGEHTDYNDGFVLPCALDMDFGVALRPVA
                     GRQARIVAADMPDAAADTFEVDQPGQGDPAQWARYARAVVWSLAEEGFVVDGFEAVIA
                     SDVPQGSGLSSSAAYEVALATALARTFGFEIALPRLALLCQRAENRFVGVQCGIMDQS
                     ISAMGRARHGLFLDCRDLSFEHVPIDTEHYRVVVCDTSAPRTLAGSAYNERRRQCETV
                     AAAIGRRHEGVRALRDATLEMLDEVAGDITPVEFRRARHVLSENGRVLEAKSLLARGD
                     AVGFGRLMADSHVSLRDDYEVSSAELDAMVEIANEVEGCVGARMTGAGFGGCTVNLVA
                     APAVEAFAAHIGRHYPERTGRTPRVYVCEAADGAGEWG"
BASE COUNT         1089 a         2381 c         2216 g         1085 t
ORIGIN      
        1 tacgacttcc tcttcgacct cacaggcctg gtagcgagcg gcgagaccgt gtcattggcc
       61 ttccgttacc tgaacccgga catcccgcga gcactgggcg tggaacgcgc gccgctggcg
      121 ctggaccgga tcgccctcga ttcgatgccc acggccgatg tggagcggcg ccggcgcgag
      181 gcgctcaagg ggcagagagt cacccgagct ccggtagagc cacgcttgcc ctccggcgcg
      241 aagcgcggcg agcgacccta tgagatcgaa tggtccggcc gccaggagtc gccgcgcgcg
      301 caggtggcct tcgacgatct tcgcggctgg acactgcacg tccttggtga cgcgtccgtt
      361 tcgctctcgg cctcggtaga gcacgtgctc tggcggccac agttggcgag gttctcctac
      421 gctggtggga gcatcgacac tacggtcgag atccgcccac cgagacccat accgatccgc
      481 ggccggttcg atgcggccaa cctgtggctc tatggggctt gggaccgggt tggcgacgcg
      541 cgactgcgca tcaccgccgt cctcgaggat caggcagggc gggagtatga gatggacctg
      601 ggcgcggtga ctgccgccta ctgggggatg cagcacggcg tgctgcgccc gagtgtgatc
      661 cgggcggctc gcttcccgat gaagtttgtc gccttgcgga tcgccaactg ccgagtgaag
      721 ggcgagcggc acgcctacct cgagtcgctc gccttctaca agcaggaccg tgagccacca
      781 cgagcccccg ctcgtccgga gccagccgtg ttccccgtgt cggatgacgg catgctgcca
      841 acgccgccgc cggggacggt cgctcgtgtc cggccgaggg gccggggggc ggagttctcg
      901 accgaggtgc ggggtgaccg gctgcagttc cgcatccggc ccgaagaggg ctgtctgcat
      961 ggtgtgacgg cccggtggaa caacgggccg cggtggcacc ccatggcgga cggcggcccg
     1021 atgttcgacc ggcgcggcgt gccgcctggc accgccaggg tggtctcgtc ggccctcaga
     1081 cacggcgtgc tgacggtgca ctggcgtgag ggcgtgggcc ggcgtgcgct cgagtgggag
     1141 gcggactact cagtgcgcgg gtgcacgctc gtcgtcgatg tgcggtgctc aggaggcaag
     1201 gccaccggcc tggctcttgg cgatgtggcg ggtctctccg acccgcgtgg gatcgaggtg
     1261 ccctacctgc tgatggggca gaagcccgga ccttggatcg cgcatgccga cgggatcttc
     1321 gtctccgtgc tccccgactg gtaccacagc gactgttcgg ccatcgacac gtcggtggtc
     1381 ccgacccgag gcgatcgcat cggcctgatg cgcggcacga gctaccagcc cctgaccgac
     1441 ggccgtcgca atcccttgcg cgaccgcatt ctcgtcaccg tctcttccga gttcgccgat
     1501 acgctgccga acgcgcggaa ccccgtctcg cccaaccgtg aacggctggc gccctacatg
     1561 ttctacatgg ccgaccaact gaaccccaac ctgtaccgca cgctgaaacg ttgcggtatc
     1621 gacaaggtga tcgccaccga cttcgcggcg ctgctcgtcg ccaaccacta tgccgagggc
     1681 ttcgcggctc gttggcggcc tcacccttcc ctcactttcg agcaagtgca ggagtaccgg
     1741 tcgggcatca agtcgctggg gtatctgttt ggcacctaca tggacgtcac cgacttctac
     1801 cccggcaacg agttctggga cgaagactgg gccgtccttg gctcagacgg cgacctgacc
     1861 gacggctggt ggggcaacta cctcgtcaag cccgccctga tgccggggct gattcgcgcc
     1921 gtcggcgaga aggcaaaggg gctctacccg tccgactgcg tgtacctcga cgtgcacacg
     1981 aaccggggcc cgcaggcgct ggacttcgag gccggagcgc ccggcgccgg catcgcgcgc
     2041 gagcaggtcg tcgcgaacgg cgactgcatc gtcgaggcac gcgagcagta tggttcgacg
     2101 atgagcgagg ggctgtatcg ttggatgtac gccgggctga ctgatatgga ttacgcgacg
     2161 ctgatcacga ccggcaccgc gtccgagctc gccccactcg tcgacttcga cctcctcaag
     2221 atccacccgt ttcagcacgg cacgatgatg gggcaccatc cggcttgtct actgagcagc
     2281 gaggagcagg cgccgctgta cgcagatacg gggcaagggc tcgggcccga gagcttctac
     2341 aagtacgtct cggccagcct ggcctacggg cacatgctca tcctggggta ctggtatacg
     2401 cctccgctgt cccgcttcat ccactacgac gcgctgatgc agggcgtgca gcgcgagtat
     2461 ctcaccgaca cagccgccga gatcggctac cacaatggca aagcgttggt gccgacgagc
     2521 caagcgctgg ccgagggttc gcagcagctt gggcgcgtgt atgtgcgcta ctcgcgcggg
     2581 ctgcgcgtct gggtcaacta caacgcggag aagccctggg ccgtggaggc gagtgggcgc
     2641 acgtatgaac tgccgcccta cggctggctc atcgagaagc cggacgagat cctcgcctac
     2701 agcgcgctga tcggcgggca ccgggtcgac ttcgtgcgtt gcccagacta cctctacctc
     2761 aacaccggtg acacgcgagc cgccgaggga cccctcgacg tcgatggcgc cgtctggctc
     2821 aaacgcgagg gcggcgggtg gcggctcatc ccctgtggag acctggggcc ttgggagcgg
     2881 ttcccacccc cgggccttcc ctcgaacttc agcgacttcc gactcagcgg cgtgccaccc
     2941 gaccgcgggt gccgcagcat cgtcgtcgac accagagcac tgctcgcgag gccggcaggc
     3001 gaggtccgca tcacggcccg cggcgaggcc ggcgaccgga cggacgctga cgtgcgccag
     3061 ctcgacgacg cgcgccttca gttcgtgtcc atggccggta ttgtggactg cgtgatggaa
     3121 tagcagacgg ccgggtgggg cgggcccggc acttgctcga catcggcagt ccttgcctca
     3181 ggccgcttca ccgagggtat cttgccctcg ccggcggcgg gggtttgccg cagggcgagc
     3241 cgagccttat cattcttcgc acatccctgc aggagtgcac tatggccacc acacgacctg
     3301 atgtcgatcc tcccgacagc ccatgctcgg tcggctggac ggtgccgcag accatccgcc
     3361 tcgcgacgcg ggaggacccc ttcccgctcg aggtcggtgg cagcatcggt cccgtcgacg
     3421 tcgagtacga gacctacggc gagctcaaca aggccagaga caatgtcatc ctcgtgacgc
     3481 atgcgctctc gggcgatgcc cacgtcgcgg gctgggacaa gacggccgag gccaccgggc
     3541 ggctctggcg tgcccgcaag cccggctggt gggacaccat ggtcggaccg ggcaagcctc
     3601 tggataccgg ccgctacttc gtggtctgcg ccaacttcct tggaagctgc tacggcacca
     3661 cggggccgtc ctcgcccaac ccttccacgg gcaagcccta cggactgagc ttccccgtgg
     3721 tgaccgtcga ggactgggtc cgtcagcaag cccggttgct cgacgccctt ggcgtcgcgc
     3781 gcatccgggc ggtcatcggc ggctcgctcg gcggccagca ggccatcgag tgggctctcg
     3841 cctaccccga gcgactcgac aagtgcatcg tgctggccgc atcacccagg ctatcgactc
     3901 agggcctcgc cttcaacgcg gtgggccgcc agacgatcat gagcgacccg aacttcaacg
     3961 gagggtgcta ctacgatggg cccgccccgg ccgatggtct ggccgccgcg cggatgctgg
     4021 ctcacatcac ctatctgtcc gagccgggca tgcatgacaa gttcgggcgc cggctgcagg
     4081 atcgcgaaca gccagcattc agcttcggga tcgagttcgc cgtcgaaagc tacctcgacc
     4141 accagggaaa ggcgttcgtc gagcggttcg acgccaacag ctacctctac atcacccgcg
     4201 ccatggacta ctacgatgcg gcggcccagt ggggcggcgg cgacctggtc gaggcgtgtc
     4261 ggcgagtgca atcggacgtg atggtcgtct ccttctcgtc cgattggctc tatccgccgg
     4321 cacactgccg cgagttcgcc accgcactgt gtcagctcgg caaacccgtg acctatgtcg
     4381 acgtcccctc gcgctacggc cacgacgcct ttctggtcga gtacgatgtg gtgggctccc
     4441 tggtccgcag tttcgtcaac aatgagaggc ggtcgtcgtg aacgagaacc ctccgatcct
     4501 cagcctcggc gatgcgtccg aactggccgc cctcatccgc gacaaagtcg accccgccct
     4561 gatgggcctc agtgccggga agctcaccga gtacctcaac cacgtcggct catcccagcc
     4621 acggaccccc ggccctgacg ccggacggct tcagcgttgg caggacgaca tcatcgagtc
     4681 caccgtgccg caaggatgct cgatcctcga tctgggctgc ggccatggcg acttgctggt
     4741 gcgcctcatg gaccggaagc aggtgcgcgg acagggcatc gagctcgacc ccgatgcggt
     4801 catccagtgt gtgaggcagg gggtccccgt ggtccaggca gacctcgacg aaggtctgaa
     4861 ggactttgcc gatgagagct tcgattacgc cgtactcgaa cagacgctcc agactgtccg
     4921 ccggcccatc gcactcctgc aagagatgct ccgagtcgcc cgccggggca tcgtctcctt
     4981 cccgaacttc gcctactggc gggtgcggct cgacctgggc ctgcgtggcc gtatgcccat
     5041 cacggagcgg ctgccatacc ggtggtacga cacaccgaac atccactcgt tcactctgca
     5101 ggacttcgag gactggactc gcacatgcgg ggtccgcatc ctgtccggcc acgtcctggc
     5161 cgagggccaa gtccgcgaac tcagagcctc ggacaacctc tatgccgagg aggtcttggt
     5221 cttcgtcgag cgcgagtagg gcgggacatg ggccgcccgc ccctatcccc actcgcccgc
     5281 accatcggcg gcctcgcaga catacacccg cggtgttctc ccggtccgct cagggtagtg
     5341 tcgcccgatg tgcgcggcga acgcctccac ggccggcgca gccaccaggt tgaccgtgca
     5401 tccaccgaac ccggccccgg tcatccgcgc gcccacgcag ccctcgacct cgttcgcgat
     5461 ctccaccatg gcatcgagct cggccgagct gacctcatag tcatcgcgga gactcacgtg
     5521 cgagtccgcc atcagcctcc caaaaccaac cgcatcccct cgcgcgagca gcgacttcgc
     5581 ctcgagcacg cggccgttct ccgagagcac atgccgcgcc cggcggaact ccaccggcgt
     5641 gatgtcgccc gccacctcgt cgagcatctc aagcgtggca tcgcgcagcg cccggacgcc
     5701 ttcgtgccgc ctgccgatag ctgcggccac cgtctcacac tggcgccgcc gctcgttgta
     5761 ggccgacccg gccagcgttc gaggcgccga cgtgtcgcag acgaccacgc ggtagtgctc
     5821 ggtgtcgatc gggacgtgct cgaacgagag gtcacggcag tcgagaaaca gcccatgccg
     5881 cgcccggccc atggccgaga tcgactgatc catgatgccg cactgcacgc cgacgaaccg
     5941 gttctcggcc cgctggcaga gaagagcgag gcgcggcagg gcgatctcga acccgaaggt
     6001 ccgcgccagt gcggtggcca gcgctacctc ataggccgcc gacgagctca ggcccgagcc
     6061 ctgagggacg tcgctggcga tgaccgcctc gaagccatcg acgacgaacc cctcctcagc
     6121 cagcgaccac acgacggcgc gggcgtagcg agcccactgg gccggatcgc cctggccagg
     6181 ctggtccact tcgaatgtgt cggcggccgc gtccggcatg tcggccgcca cgatgcgggc
     6241 ctggcggccc gccactggcc gcagcgcgac gccgaagtcc atgtcgagtg cgcagggcag
     6301 cacaaagccg tcgttgtagt ccgtatgctc gccgatcagg ttcacgcgcc ccggggcgcg
     6361 gaagtaccga aacccctggg ggccgcccgg catcgagccc agcgccccgc gaagccgatc
     6421 cacaaccgcc atgtccacac ttaccctcag ccgcagactg tgcgcatcgc gcgcgcggcc
     6481 attgtacgac gcggcgcacg gcagcgtcac tgcggctcgc cgatggatcg gccgcaggcc
     6541 gacagcgccc tccatcggtc cgcgcaagcc tccacccccg ccgttcctga gccgccgcgc
     6601 ccgcgcggcg tgacgaaggg cagcggcacc atgccccgtc ctgccccttc gtgatgcgcc
     6661 catcgccggg cactgaagtg caccccaatg agtagacagg gattgaggcg aagttagggg
     6721 tatgggtctc tggagaccgg gccggatggc gcccttcccg ctgcgcgggc c
//
LOCUS       DAOSPL010000034         6754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_11363_length_6754_cov_3.176892, whole genome shotgun sequence.
ACCESSION   DAOSPL010000034 DAOSPL010000000
VERSION     DAOSPL010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11363_length_6754_cov_3.176892"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PLQ54_01165"
     CDS             <1..527
                     /locus_tag="PLQ54_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91898.1"
                     /translation="DAPLVSLCDINTDKWLKKLDLSNQTIYSYIMNNYWFTNYKAGQG
                     GRLIFRYSLTSHRGPYDAVAASHFGAEASAGLMVWRGADDMHRVVEVGDPRLTLLSIA
                     PVDPSATPRKVVMRLWNVSDSTVTAPVRIAGHAVTSIREVRLTAEPVGPAKGAAEVTC
                     KPRRVHAFELTLDD"
     gene            544..1461
                     /locus_tag="PLQ54_01170"
     CDS             544..1461
                     /locus_tag="PLQ54_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS91899.1"
                     /translation="MTIRAMKTSDIEPLHRLYSRLAARIPNHPKVSLEQFATELKGFG
                     TGRMEQRTAGAPRRAFVAVRTGLPVAFASTAVCTEGFWELATGQGVLRFVFAPPGEQE
                     ACVELIRRATARIRADGGERPMACVYGSGPGFHNWACAMVPSAWPWIGHWLCMAGYAP
                     AGSEIRMRRVLEKRPRPIALPKGSELRVATAPESDRVAEEFQHHRHLWIDGQEAAQCH
                     NFYEERFVRGRGKRMSYTEWLGVNEAFRGRGLGRAMLRNAVCRAYDEGCRVVTLTTDG
                     CNFHAQALYVSEGYAQTDTMWRFGWGEEG"
     gene            complement(1561..3126)
                     /locus_tag="PLQ54_01175"
     CDS             complement(1561..3126)
                     /locus_tag="PLQ54_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91900.1"
                     /translation="MMRWMRSTLARATASVLCLALLSATVPSCGSGAPPVDGNNGNAN
                     GGNNNGGSGNGGVGTPTVTTVDERSAYATSTTGNALGASVVPGGAYTGGFSLIVTARD
                     ADEMGMLAVGPLAGAANGDLTGVTVIVKNPSGAEVARLGDGSVRYAAGLTSIPEPAAA
                     VTTGLDDFLTNTVGVDPTADPDTNVQKLVDYWANGDNEPPSDAVETGLTAYVGALKAA
                     SNAVGPDVDGATRPTAVVLNPAVGTWTVEVTSADAAAAYGVTGLYSQSRSRIADMIGM
                     QNALPNVEPASVRVGKDDPECQKVLDSFAIDMLSFLPLELAGVANPGLPGLVSWIIGI
                     GVGVFFIEAAGAGAVGFVFGLAFSYLLGKTIDYAFQQGGHQDTWGDAFKMGLSSLVDR
                     DPAGLSLNVFFPPGPYNPKTQVLYTGPSREFTVRRLFVNHCGALALGFSFPGTTTWKL
                     HDGSGGATTMGSVIETESSSGSYLKVRTAARGESVAAGNFYIEARHRIAADQPEMVST
                     VRVIYDTNPLIFP"
     gene            complement(3225..4211)
                     /locus_tag="PLQ54_01180"
     CDS             complement(3225..4211)
                     /locus_tag="PLQ54_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS91901.1"
                     /translation="MNHVERFRAIMDFQPVDRLPRWEWAMWWDQTIANWRQQGLPHAL
                     QSTLEIAEYFGLDPYQQFWFSTTDPTIEAVQHHLIGSVSCMDDYTNLRPGLFPEHRAA
                     IEAMAPWRDRQARGEVVVWATLEGFFWFSRTLMGFTNVSLAFYDQPELIHQMNQDLAD
                     FNVRLIEQIQQACVPTFVTIAEDMSYNHGPMLSERHFDEFLAPYYRQVVPRLLELGII
                     PIVDTDGDVTQMVPWLQRVGIRGVLPLERQAGVDGMALRQQFPGLCMVGHFDKMVMDK
                     GEDALRREFERLMPLMRSGGFIPSVDHQTPPHISLDQYWLYRRLLDEYTELI"
     gene            4489..4773
                     /gene="rpmE"
                     /locus_tag="PLQ54_01185"
     CDS             4489..4773
                     /gene="rpmE"
                     /locus_tag="PLQ54_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004017962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOS91902.1"
                     /translation="MKDKIHPEYVDCHVTCACGHDFMTRGTKAEIKVEICSACHPFYT
                     GKQKIVDTEGRVDRFRQRQILAQQLQQEAEKRAEKKARAAKPKEAAPSAG"
     gene            complement(4855..5976)
                     /locus_tag="PLQ54_01190"
     CDS             complement(4855..5976)
                     /locus_tag="PLQ54_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS91903.1"
                     /translation="MTADRARELTDLWAAFSKGCNTRVPLTFACDEQLWLRVSGHTFR
                     EFYTRPEVHLKAQLEGKRWFCEHVEGDMPPGLPDEWALAVQLWMEEDEFFGCEVIYQE
                     DDYAWARPLDLDNHSLLAWIADLDPEARVRGGHAFHLYEAVSELADGMEYLGRPVRVV
                     PPGQGTHGVFTKAAEVRGLERLCLDLVESPGFAEQFLRLMTEKTIERIRAWHRVTTGA
                     ELQLPHAGGWGCCDDSLQMIGPHVYADLVAPCHERLYSAMSTGRRSMHLCGHAEQHYV
                     ALHDRLGVTILDGPGPFVDHAYYLERFGPDFRFAAQTDHSILERGTPAQVREMMRRLL
                     TPGAKQPGRFSLLGYVTRHTPLENVRACYEAALEFGRIQ"
     gene            complement(5973..>6754)
                     /locus_tag="PLQ54_01195"
     CDS             complement(5973..>6754)
                     /locus_tag="PLQ54_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOS91904.1"
                     /translation="VMRDFRTTVLVGTPDYALTIAGAMEDAGLDPHELSLRVGIFGGE
                     PWDESVRQTVENRLQLRAFDCYGLAELGGPSVSFECPQHSGLHIAEDQFLVETVDPVT
                     GEPLTAGEEGELVFTTLAKEAFPLLRFRSGDISRIDETPCPCGRTHARMARVGRHADD
                     RVVVRGVCIVPGQIEALVGSVPGLSPACRIVVDRSSLLERIEVHVQLAPGTVPDRVGR
                     LIQMEAEVRRKLEETLGLPVDVKLGAPGTLPAGAPALEEAS"
BASE COUNT         1132 a         2161 c         2366 g         1095 t
ORIGIN      
        1 tcgatgctcc gttggtcagc ctgtgtgaca tcaacactga caagtggttg aagaagctgg
       61 acctgtcgaa ccagacgatc tactcgtaca tcatgaacaa ctactggttc acgaactata
      121 aggcaggtca gggcgggcgg ctgatcttcc ggtactcgct gacgagccat aggggcccct
      181 acgacgccgt ggcggcctcg cacttcggcg cggaggcgtc agcggggctc atggtctggc
      241 gcggggccga tgacatgcac cgcgtcgtcg aggtgggcga tccgcgcctg acgctgctct
      301 cgatcgcccc ggtggaccca tcggccacgc cgcggaaggt ggtcatgcgg ctgtggaacg
      361 tctcggacag cacggtaacg gcgccggtgc gcatcgccgg gcacgcggtc acatcgatcc
      421 gtgaagttcg actcaccgcc gagcccgttg ggcccgccaa gggggccgcc gaggtgacct
      481 gcaagccgag gcgcgtacac gccttcgagc tgacgctgga cgactgacga aggggccatc
      541 cagatgacaa tccgcgcgat gaagacctcc gacatcgagc cactgcatcg gctctactcc
      601 cgcttggccg cccggattcc gaaccacccc aaggtgtcgc tggagcagtt cgccactgag
      661 ctgaagggct tcggtacggg gcgcatggag cagcgaacgg ccggggcgcc acgccgggcg
      721 tttgtcgcgg tgcggacggg tctgcccgtt gcatttgcat cgacggcggt gtgcaccgag
      781 ggcttctggg agctggccac cgggcagggc gttctgcggt tcgtgttcgc cccgcccggc
      841 gagcaggagg cctgcgtcga gctgatccga cgcgccacgg cgaggatccg tgccgacggc
      901 ggcgagcgcc ctatggcctg cgtctatggt agcggccccg gcttccacaa ctgggcctgc
      961 gccatggtgc cctcggcctg gccgtggatt ggccactggc tatgcatggc gggctacgcg
     1021 ccggcgggca gcgagatccg gatgcgccgg gttctcgaga agcggcccag gcccatcgcg
     1081 cttccgaagg gctcagagct ccgagtcgcg actgcccccg agtccgatcg ggtggccgag
     1141 gagttccagc atcaccgcca cctgtggatc gacggccagg aggccgccca gtgccacaac
     1201 ttctacgagg agcggttcgt ccgcggcagg ggcaagcgca tgtcctatac tgaatggctc
     1261 ggggtgaacg aggcctttcg aggccgcggc ctgggccggg ccatgctccg caatgcggtg
     1321 tgccgcgcct acgacgaggg ctgccgcgtc gtcacgctga ccaccgatgg ctgcaacttc
     1381 cacgcccagg cgctctacgt gagcgagggc tacgctcaga ccgacaccat gtggcggttt
     1441 gggtggggcg aggaagggta gcgcatgacc aacaggaccc gctgctgcct cgcacagtac
     1501 acagaccatg cggacagagt ccctcgcccg atgggccggc gctccggccc gtcgtgccgg
     1561 ctacgggaag atcagcgggt tggtgtcgta gatcacccgc acggtcgaga ccatctcagg
     1621 ctggtcggcg gcgatgcggt gtctggcctc gatgtagaag ttccccgccg ccaccgactc
     1681 gccacgggcg gccgtgcgga ccttcaggta ggagcctgac gacgactccg tctcgatgac
     1741 gctccccatg gtggtggcgc cgccgctgcc gtcgtggagc ttccaggtgg tcgtgccggg
     1801 gaagctgaag ccaagggcca gcgcgccgca gtggttgacg aacaagcgcc gaacggtgaa
     1861 ctcgcggctc gggcctgtgt agagcacctg ggtcttcggg ttgtaggggc caggagggaa
     1921 gaacacattc agcgacagcc ccgcggggtc gcggtcgacc agagacgaga gccccatctt
     1981 gaaggcgtcg ccccaggtgt cctggtgccc gccctgctgg aaggcgtagt cgatcgtctt
     2041 gccgagcagg taggagaagg ccagcccgaa cacgaagccc acggctcccg ccccggccgc
     2101 ctcgatgaag aacacgccca cgccgatgcc gatgatccag ctcacgaggc ccggcagccc
     2161 cgggttcgcc accccggcca gctcgagtgg caagaaggag agcatgtcga tcgcgaagga
     2221 gtccaggacc ttctggcact cggggtcgtc cttgcccacc cggacgctgg ccggttcgac
     2281 gttcggcagc gcattctgca tgccgatcat gtcggcgatc ctcgagcggc tctgcgagta
     2341 gaggccggtg accccatagg ctgcggcagc gtccgccgag gtgacctcca ccgtccaggt
     2401 gcccacagcg gggttgagaa ccacggccgt gggccgcgtc gcgccatcga cgtcggggcc
     2461 cacggcattg ctggcggcct tcagcgcccc cacgtaggct gtgaggcctg tttcgaccgc
     2521 gtcggacggc ggctcgttgt cgccgttggc ccagtagtcg accagcttct gaacgttggt
     2581 atccggatcg gctgtgggat cgaccccgac cgtgttcgtg aggaagtcat cgaggcccgt
     2641 ggtgacggcg gccgccggct cggggatgga tgtcagtcct gcggcgtacc tcaccgagcc
     2701 gtcgccgagg cgggcgacct cggcacccga cgggttcttg acgatgaccg tcacaccggt
     2761 caggtcgccg ttcgccgcac cggccagagg acccacggcg agcatgccca tctcatcggc
     2821 gtcccgggcg gttacgatca ggctgaagcc ccccgtgtag gcccctcccg ggaccacgct
     2881 ggcccccagc gcgttgccgg tggtcgatgt ggcgtaggcg gaccgctcgt cgaccgtcgt
     2941 gaccgtcggg gttccgacgc cgccgttgcc gctcccgccg ttgttgttcc caccgttagc
     3001 attcccgttg ttgccatcga cgggcggtgc accagagcca cacgagggga ccgttgcgga
     3061 gagcagagcc aggcacagca ctgaagcagt cgcgcgggcc agagtggagc gcatccatcg
     3121 catcatgagc gttgccatct cctctcgaat ccgacaagcg cgccacatca tgcaccacaa
     3181 tgcttgccag caccgatacg ctacggattg ttccgggtcg gttcttagat cagctccgtg
     3241 tactcgtcga gcagtcgccg gtagagccag tactggtcga gggagatatg cggtggtgtc
     3301 tggtggtcga cgctggggat gaaccctccg ctgcgcatga ggggcatgag ccgctcgaac
     3361 tcacgccgca aggcgtcctc gcccttgtcc atgaccatct tgtcgaaatg gcccaccatg
     3421 caaagccctg ggaactgctg gcgcagcgcc atgccgtcga ccccggcctg gcgctcgagc
     3481 gggagcacgc cccggatgcc gacacgctgg agccagggca ccatctgggt gacatcgccg
     3541 tcggtgtcga cgatggggat gatgccgagc tcgagcagcc gtgggacgac ctgccggtag
     3601 tagggggcca ggaactcgtc gaagtgccgc tcagagagca tgggcccgtg gttgtacgac
     3661 atgtcctcgg caatggtgac gaaggtcggg acgcaagcct gctggatctg ctcgatgagt
     3721 cgcacgttga agtcggccag gtcctggttc atctgatgga tcagctccgg ctggtcgtag
     3781 aaggccaggc tgacgttggt gaagcccatg agggtgcggg agaaccagaa gaagccctcg
     3841 agggtcgccc acacgaccac ttcgccgcgg gcctgacggt cccgccacgg tgccatggcc
     3901 tcgatggccg cccgatgctc ggggaagagc cctgggcgca gattggtata gtcatccata
     3961 caactgaccg acccgattag atggtgctga actgcctcga tggtcgggtc agtggtcgag
     4021 aaccagaact gctggtaggg atcgaggccg aagtactcgg cgatctcgag tgtgctctgc
     4081 agtgcatggg gaaggccctg ctgacgccag ttggcaatgg tttggtccca ccacatggcc
     4141 cactcccagc gcgggaggcg gtcgacgggc tggaagtcca tgatggcgcg gaaacgctcg
     4201 acatggttca cgggcagggc tccttccggc tggatatggg caccggagca gcagcgcgtg
     4261 gcggttcgcc gtaagggagc cgggtcctgc tgtcgaccgt gggtgtggga gctccggcgg
     4321 gccgccttgg gctacgcgga ccggtcgcag gcgccccgtt ttcggggttc aggagaccgg
     4381 cgatggtcgc cggtcttcgg gcactgagat ttgtttgccg cgtgggcagg tcgactgcta
     4441 tcattagagt tcactgtgtc ttgtgggaac ccgagggaat gaagcgccat gaaggacaag
     4501 atccatcccg agtatgtcga ttgccacgtg acctgcgctt gcgggcacga cttcatgact
     4561 cgcggcacca aggctgagat caaggtcgag atctgctcgg cttgccatcc gttctacacg
     4621 ggcaagcaga agatcgtcga cactgaggga cgcgtggacc ggttccggca gcggcagatc
     4681 ctggcccagc agcttcagca agaggcggag aagcgggccg agaagaaggc ccgggcggcc
     4741 aaacccaagg aggctgcccc ttcggcgggc tagccgcggt gggttaggac agggactcgg
     4801 agagcgccgg cagcgacagc gccggcgctt tgtgtgcgta cgctctgcgg ggcgtcactg
     4861 gatccgcccg aactcgagcg cggcttcgta gcaggcccgg acgttctcga gcggcgtgtg
     4921 gcgtgtgacg tagccgagca gcgagaagcg ccccggctgc ttggcaccag gagtcaggag
     4981 gcggcgcatc atctcacgaa cctgggcggg ggttcctcgt tcgaggatgc tgtgatcggt
     5041 ctgggccgcg aaacggaagt ccggcccgaa ccgttcgagg tagtacgcgt ggtcgacgaa
     5101 ggggccggga ccgtcgagga tggtgacgcc cagccggtcg tggagggcca catagtgctg
     5161 ctcggcgtgc ccacacaagt gcatggagcg cctcccggtg ctcatcgccg agtacagccg
     5221 ctcgtggcac ggggccacca ggtcggcgta gacgtgaggg ccgatcatct ggaggctgtc
     5281 gtcgcagcag ccccagccgc cggcgtgggg cagttggagt tccgcacccg tggtgacgcg
     5341 atgccaggcg cggatccgct cgatggtctt ctcggtcatc aaccgcagga actgctcggc
     5401 gaagcccggc gactcgacca gatcaaggca caggcgctcg aggcctcgga cctcggcggc
     5461 cttggtgaac accccgtggg tgccctgccc gggcggcacg acgcgcaccg gacgacccag
     5521 gtactccatg ccgtcggcca gctcgctcac ggcctcgtag aggtggaacg catggccgcc
     5581 tcgaacgcgc gcttccgggt cgagatcggc gatccaggcg agcaggctgt ggttgtcgag
     5641 gtcgagaggt cgggcccagg cgtagtcgtc ctcttggtag atcacctcgc agccgaagaa
     5701 ctcgtcctcc tccatccaga gctgtacggc gagggcccac tcatcaggca agccgggagg
     5761 catatcgccc tcgacatgct cgcagaacca gcgtttgcct tcgagctggg ctttcagatg
     5821 cacttcgggc cgcgtgtaga actcgcggaa cgtatgcccc gacacgcgaa gccagagttg
     5881 ctcatcgcac gcgaaggtca ggggtacgcg ggtgttgcag ccctttgaga aggccgccca
     5941 aaggtcggtg agctcacggg cacggtccgc ggtcacgagg cctcctcgag cgccggtgcg
     6001 ccggccggga gcgttcccgg cgcccccagc ttcacgtcga ccggcaagcc cagggtctct
     6061 tcgagcttgc gccgcacctc cgcttccatc tggatgagcc ggccgacgcg gtcgggcacg
     6121 gtgcccggcg cgagctgaac atggacctcg attcgctcga gcagggacga gcggtcgacc
     6181 acgattcggc aggccgggct caagccaggg accgagccga ccagggcctc gatctggccg
     6241 gggacgatgc acactcctcg gaccaccact cggtcatcgg catgccggcc aacgcgagcc
     6301 atgcgggcat gggtccggcc acagggacag ggcgtttcgt cgatccggct gatgtccccg
     6361 gagcggaagc gcagcagcgg gaatgcctcc ttggccagcg tggtgaatac cagctcgccc
     6421 tcctcgccag ccgtcagggg ctcgccggta accgggtcga cggtctcgac caggaactga
     6481 tcctcggcaa tgtgaaggcc ggagtgctgg gggcactcga aggagaccga gggccccccg
     6541 agttcggcca aaccgtagca gtcgaaggcg cggagctgga gtcgattttc gacggtctgg
     6601 cggaccgact cgtcccaggg ctcgcctccg aagatgccga cacggaggga gagctcgtgg
     6661 gggtcgaggc cggcatcctc catcgccccg gcgatggtca gtgcgtagtc gggggtgccg
     6721 acgagcacgg tggttcggaa gtcccgcatg acca
//
LOCUS       DAOSPL010000035         6750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_11371_length_6750_cov_2.913966, whole genome shotgun sequence.
ACCESSION   DAOSPL010000035 DAOSPL010000000
VERSION     DAOSPL010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11371_length_6750_cov_2.913966"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PLQ54_01200"
     CDS             complement(<1..389)
                     /locus_tag="PLQ54_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006752631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOS91905.1"
                     /translation="MPPRSPKPRLTTDARRRAAELSTRLLGFYDREGRDLPWRQTKDP
                     YRVWVSEVMLQQTQVVTVLPYYARFLEAFPTVRALAESATDDVLAMWAGLGYYGRARN
                     LQAAARVVVANHGGVFPRDWADALALPG"
     gene            506..1744
                     /locus_tag="PLQ54_01205"
     CDS             506..1744
                     /locus_tag="PLQ54_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91906.1"
                     /translation="MTAVDRQVLRDLAHRVAEMAATPEHQGRPELWYAQNRLKPIRPL
                     VFCSPEGSWPELIPDSDMRCEDADARGIEWGLRMRLYAAEHFHDDQVCDNHFRVGPAV
                     SSTGWGVGPEYVHPEAARGAYVWDPPIKQREDIDRIQTPKSTHDSEATARALAFHQDL
                     FGDILEVYVHGTFWWGLGLIDEWTFLRGIQQTYLDMTDDPELVHAGMRRLMEGRLAWL
                     RELETLGVLSLNNGNSYVGSGGFGFSDQLPADDFSGSVRLKDLWGFCEAQTMSEVSPA
                     MHEEFVLEYQLPILELFGLNCYGCCEPLHRKLDLLLRRVPRLRRVSISPWADKRVSAE
                     ALEDRVIFSWKPNPAALAAVEFDPEWVRRDIRETVEICRANGCVLEIIIKDTHTCNHQ
                     PKRFDDWTRIAMEEAVRGAE"
     gene            1756..2745
                     /locus_tag="PLQ54_01210"
     CDS             1756..2745
                     /locus_tag="PLQ54_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOS91907.1"
                     /translation="MKIVMTGSRRAWIEDAPDPTPEGEWVVVRATAVPICGSDMHGYY
                     AEGAHTGGHEAVGEVVAVDSAHRLRVGDRVLVHPASGCGQCWLCRRGDYIHCRAMPAG
                     SGPHFAQYIRKQEWLCPRLPDDVEDDLASLMGCGLSPAYQALTRLGVNAFSTVLITGL
                     GPVGLGAVALASFLGARVWAVDTVAWRRQRAERLGAEAALDPNQTDVLGFVRDHTCGE
                     GVPAAIDASGHPEAERLCIDALRALGKVAFIGENQGDIAYSPSRDGIRRGIEIIAAWH
                     QNLNAFADLITFLRRFPQASDLISHTFGLADVDEAFRVFASREAAKVVLHPWA"
     gene            2834..3172
                     /locus_tag="PLQ54_01215"
     CDS             2834..3172
                     /locus_tag="PLQ54_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91908.1"
                     /translation="MLPPVGLSPQLVRESYVRAEALRRTGATGYRPLDREANRSPTAE
                     HAESGSRLRSSSSSSRSSRDRAGERVVTETRVEVIEAVPTDGPRPSRPPSPFLEAQAR
                     RAYSRFAPQD"
     gene            complement(3169..3738)
                     /locus_tag="PLQ54_01220"
     CDS             complement(3169..3738)
                     /locus_tag="PLQ54_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91909.1"
                     /translation="MKVAFIRSVRIGIPEVVILKQTARSGALVMAKQGFSTRQLGAIA
                     HRAVSQARCPVFTVRAYRAPRRVLLLHDDASGCVQLAAALRELLNPAVSSVEILAAHA
                     DVVDPVMELLADAGLTAEVRRERAPLLEAALAAAAEGVDLLALTGNGRVPAGLLRSEL
                     SAVRAARRAHCMTATFATAVTRRGAGRHA"
     gene            3799..4029
                     /locus_tag="PLQ54_01225"
     CDS             3799..4029
                     /locus_tag="PLQ54_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91910.1"
                     /translation="MKISVFLTEAAHVLQGLGRGEARFQVPDVDDVDKVKPVAMAGQV
                     HRHPQCVARRRRAIGGNEDVHRAVEVLLAGGP"
     gene            complement(4118..4579)
                     /locus_tag="PLQ54_01230"
     CDS             complement(4118..4579)
                     /locus_tag="PLQ54_01230"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOS91911.1"
                     /translation="MHVAEMTGDLVAGVGAQRRGAERCRACLPVRVSAHHGPMSRGEQ
                     LWHEGFLVDLCIGGARLRAGTSFDVGDLIELELVRPNRRNCCVGQRLIAVGRVAWTRR
                     LRRGECTGTPPLHSGWRGPQEIGVEFVTLDPCLVSWFREMTEGDSSSPQSR"
     gene            complement(4904..5437)
                     /locus_tag="PLQ54_01235"
     CDS             complement(4904..5437)
                     /locus_tag="PLQ54_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOS91912.1"
                     /translation="MTRAVGEAVLELVEGNIVDEVTDAIVNAANRGLRGGGGVDGAIH
                     SAGGPAIMAECRRIGGCETGDAVITTGGALKARHVIHTVGPVYSGSARDAELLASCYR
                     RSIEVADANGLASVAFPSISTGAYGYPLDEAAEVALRATVDAMRAHPSVRKVRFVLYG
                     ATAYQAYASALERLLPR"
     gene            5537..6352
                     /locus_tag="PLQ54_01240"
     CDS             5537..6352
                     /locus_tag="PLQ54_01240"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOS91913.1"
                     /translation="MKVFISADIEGVNGVMGETHWSSQGSAYDHARAWMAEEVNAAVR
                     GACAGGASEIVVKDSHGPGTNILAHQLDPRAQLISGWGPLHSMVEGVDESFGAAFLVG
                     YHAKVGTERGVLSHTIGSTVRGLRLNGEAIGETALSALYCGHHGVPVVLVTGDDELAA
                     EAQHFLPGVATAVVKWGLNRGSARMLPLAQGRARIEEAAAEATARAGSIEPFRPSLPL
                     GLELDVPSAAAAALCALVPGVERTADCTVACSCGDAVEYVRMFRVLLNLASTA"
     gene            6371..>6750
                     /locus_tag="PLQ54_01245"
     CDS             6371..>6750
                     /locus_tag="PLQ54_01245"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HOS91914.1"
                     /translation="MRSMTTVVLRLFLASVVICGVASAQEASLAQRLGFTDEDIVLII
                     NGDDLGMCHSANEAVFDALGRGCLTTATLMVPCPWAIEVAHRWKEQPDLDIGLHLTHT
                     AEWRHYRWGPVASRHLVPGLIDAEG"
BASE COUNT         1105 a         2261 c         2295 g         1089 t
ORIGIN      
        1 cccgggaggg ccagggcgtc cgcccagtcc ctggggaaca cgccgccatg atttgcgacc
       61 accacgcgcg cggccgcctg caggttgcgg gcccggccgt agtaacccag gccggcccac
      121 atggcgagca cgtcgtccgt cgcggactct gccaacgcgc gaaccgtcgg gaatgcctca
      181 aggaaccgcg catagtaggg cagcacggta acgacctggg tctgctgcag catgacctcg
      241 gagacccaca cgcggtaggg gtccttggtc tggcgccagg gcagatcgcg cccctcgcgg
      301 tcgtagaagc caagcagcct cgtcgagagc tcggcggcac ggcgccgggc atcggtggtc
      361 aggcgaggct tcggggaacg aggtggcatc cgttgccatc gtaacccgct tgaggcgagc
      421 gcaccagagg cggcaggacg gtcgtttggt tccgcgaaca gtcccgagac gttctggatg
      481 tccacacgag aggtgacggc ccgtcatgac agcagttgat cgccaggttc tccgcgacct
      541 ggcccaccga gtcgccgaaa tggccgcgac ccccgagcat cagggccggc ctgagttgtg
      601 gtacgcgcag aaccgcctca agcccatccg gccgctggtg ttctgttcgc ctgagggctc
      661 gtggcccgag ctgatcccag actcggacat gcggtgcgag gatgccgatg cacggggcat
      721 cgagtggggg ctccgcatgc ggctctacgc cgccgagcac ttccatgatg accaggtgtg
      781 cgacaaccac tttcgcgtgg gcccggcggt cagctcgacg gggtggggag tcggaccgga
      841 gtacgtccat cccgaagccg cacggggtgc ctacgtctgg gatcctccga tcaagcagcg
      901 agaggacatc gaccgcatcc agacgcccaa gagcacccat gactccgagg ccacggcccg
      961 ggctctcgct ttccaccagg acctgtttgg cgacatcctc gaggtctatg ttcacggtac
     1021 cttctggtgg ggcctggggc tcatcgacga atggaccttc ctgcggggca tccagcagac
     1081 gtatctcgac atgacggacg accccgagct ggtccatgcc gggatgcgcc gcctgatgga
     1141 gggtcgcctt gcctggctcc gggagctgga gacgctcggt gtgctgtcgc tcaacaacgg
     1201 caacagctat gtgggctcgg gaggcttcgg tttcagcgat cagctgcctg cggacgactt
     1261 ctcgggttcg gtgcgcctca aggacctatg gggcttctgt gaggcgcaga ccatgtcgga
     1321 ggtctcgccg gcgatgcacg aggagttcgt gctcgagtac caacttccca ttctcgagct
     1381 cttcgggctc aactgctatg gctgctgcga gccgttgcat cggaagctgg acctgctcct
     1441 gcgacgcgtg ccccggctgc ggcgcgtgtc gatcagcccc tgggcggaca agcgggtgag
     1501 cgccgaagct ctggaggacc gggtgatctt ttcgtggaag cccaatccag cggcactggc
     1561 ggcggtcgag ttcgaccccg agtgggttcg gcgagacatc cgagaaaccg tcgagatctg
     1621 ccgggcgaac gggtgcgtgc tcgagatcat catcaaggac actcacacgt gcaatcacca
     1681 gcccaagcgt ttcgatgact ggacccgcat cgccatggaa gaggcggtcc gtggggcgga
     1741 gtaggggaca accggatgaa gatcgttatg accggcagcc ggcgagcctg gatcgaggac
     1801 gctcccgacc cgacgccgga gggcgagtgg gttgtggtga gggcgaccgc cgttcccatc
     1861 tgcggcagcg acatgcacgg gtactatgcc gagggcgcgc acacgggagg ccatgaggct
     1921 gtcggtgaag tcgtggccgt cgactcggcc caccgcctcc gcgtgggcga ccgcgtcctc
     1981 gtccatcccg ccagcgggtg cggccaatgc tggctctgcc ggcgaggcga ctacatccac
     2041 tgtcgcgcca tgcctgcggg gagtggccct cacttcgcgc agtacatccg caagcaggag
     2101 tggctgtgcc ccaggctgcc cgacgatgtg gaggacgacc tggcatcgct gatgggctgc
     2161 ggactctctc cggcctatca ggctctcacg cgcttgggcg tgaacgcgtt cagcacggtg
     2221 ctcatcacag gcctggggcc cgtggggctc ggggccgtgg ctctggcgag ctttctcggc
     2281 gcccgtgtgt gggcggtcga caccgtcgcg tggcggcgcc agcgcgccga gcgcctgggc
     2341 gcggaggcgg ctctcgaccc caaccagacc gatgtgctcg gtttcgtccg cgaccacacc
     2401 tgcggcgagg gagtacccgc cgccatcgat gcctccggcc accccgaagc cgagcggctg
     2461 tgcatcgacg cgctgcgggc cttgggtaag gtggcgttca tcggcgagaa ccagggcgat
     2521 atcgcgtaca gtcccagccg ggacggcatt cgccgcggca tcgagatcat cgccgcgtgg
     2581 caccagaacc tgaatgcgtt cgccgacctg atcacgttcc tgaggcggtt cccacaggcg
     2641 tcggacctaa tcagtcacac atttggactc gctgacgtcg atgaggcatt ccgcgtcttc
     2701 gcgtcccggg aggcagcgaa agtggtactg cacccgtggg cgtgacgagg agcgcggcgc
     2761 gaggatggtg ggcctggatg tggaatgcct gtaatggctg cgtgaagcat gcacggggcg
     2821 aggaggtgag ccaatgctgc cgccagtggg tctatcgccc cagcttgtcc gcgagagcta
     2881 tgtcagggcc gaggcgctcc gcaggaccgg cgcgactggc taccgccctc tcgatcgcga
     2941 ggccaaccgg agccccactg cagaacatgc cgagagcgga tccagactgc gctcttcgtc
     3001 gagctcgtca cgatcgtcgc gcgacagagc cggcgagcgg gtagtcaccg agactcgggt
     3061 ggaagtgatc gaagccgtgc ccaccgacgg cccaaggcca tcccgcccgc cttcgccgtt
     3121 ccttgaggcc caggcccggc gggcgtattc ccggttcgcc ccacaggact aggcatggcg
     3181 gcccgccccg cggcgggtta ccgcggtcgc gaatgttgcc gtcatgcagt gggcgcggcg
     3241 ggccgctcgg accgctgaga gctcgctgcg caggaggcca gcgggcaccc gaccattgcc
     3301 cgtcagtgcc aacaggtcga ccccctcggc cgctgcggcc agcgcggcct cgagcagggg
     3361 agcgcgctcg cgccgcacct ccgccgtgag cccggcgtcg gcgagaagct ccatgaccgg
     3421 gtccacgaca tcggcatggg ccgcgagtat ctccaccgag gacacggccg ggttcagcag
     3481 ctcacgcagc gccgccgcca gctgcacgca gccagaggca tcgtcgtgga gcagcaggac
     3541 gcgccgcggg gctcgatagg cccgcaccgt gaacacggga caccgagcct gcgacaccgc
     3601 gcggtgcgcg atggcgccca gctgccgggt cgagaacccc tgcttggcca tgaccagtgc
     3661 cccggaccga gccgtctgct tgaggatgac aacctcgggg atgccaatcc gtaccgaccg
     3721 gatgaaggcg accttcatat cggcgcagat gtcggctgcc ccatcgagga tctctcgggc
     3781 ctcggccgat taggcagctt gaagatcagc gtcttcctca ctgaggccgc ccacgtcctg
     3841 cagggcctcg ggcgaggcga ggcgaggttc caggtaccgg atgtcgacga cgtggacaag
     3901 gtgaagccgg tggcgatggc cggccaggtc catcgccacc cgcaatgcgt ggctcgccgc
     3961 cgtcgagcca tcggtgggaa cgaggatgtc catcgtgccg tcgaggtgct cctcgccgga
     4021 ggcccgtgac gccgaacggg tccgcccctc cgggcttcag gccggtcgac gcgctctgcg
     4081 aatgatcact agttgtccgc ttcgaccatt ccggaaccta gcggctttgg gggctgctcg
     4141 agtccccctc ggtcatctca cggaaccacg agaccaagca ggggtccagt gtgacgaact
     4201 cgactccgat ctcttgaggg ccgcgccagc ccgaatggag gggcggagtg cccgtgcact
     4261 cgcctcggcg gaggcggcgg gtccaagcga cccgtccaac cgcaatcagg cgctgaccca
     4321 cacagcagtt cctccggttg ggccgcacaa gctccagttc gatcaggtca ccgacatcga
     4381 aggaggtacc ggcacgcaga cgtgccccgc caatgcagag atctactagg aagccttcgt
     4441 gccacagctg ctcgccgcgg ctcatcggcc catggtgggc cgagactcgc acgggcagac
     4501 acgcccggca ccgctctgcc cctcgcctct gcgccccaac cccagcaacc agatccccgg
     4561 tcatctcagc tacgtgcact acgtatcgtc tctctctcaa gcagtctcgc gcccgtgact
     4621 accttgagga tatcccgatc ctcaaagact tcagcggccg agtcaccgct ccggccgcct
     4681 cgagggcccc agcgtcagcc cgtgtccgct ccagattcct gctcgccgcg gcgtgggcat
     4741 ggcacctcta gtctggttaa ccccagcggg aggctagcga tatcccatcg gcgtgtcaac
     4801 cactaacgca agcacccacc atgaccttcg ggcactcccc ggggcgtatc cttccacagt
     4861 attgcctacg acggacgccg gcgcgccaca cgggtacccg gcgtcaccgg gggagcagtc
     4921 tctcgagcgc cgacgcatag gcctgatagg cagtggcgcc atacagaacg aaccgaacct
     4981 tgcgcacgct gggatgggct cgcatggcgt ccaccgtggc tcggagcgct acttcggcgg
     5041 cctcgtcgag cgggtagccg taggcgccgg tgctgatcga tgggaatgcc acgctcgcca
     5101 ggccgttggc atccgccacc tcgatgcttc gccggtagca gctcgcaagt agctcggcat
     5161 cacgcgcaga cccggagtag accgggccca ccgtgtggat cacatgccgc gccttgaggg
     5221 caccgccggt agtgatcacc gcatcgccgg tctcgcaccc cccgatccgg cggcattcgg
     5281 ccatgatggc gggtccgccg gccgagtgga tggcgccatc gaccccgccg ccgccccgaa
     5341 gcccccggtt cgcggcgttc acgatggcgt cggtcacttc atccacaatg ttgccctcga
     5401 ccagttcgag gactgcctcg cccacggcac gcgtcatgga cttcatcccc ttcgcgtaga
     5461 ggagcgttcg ctcttggccc gaagctctcc tgggccggcc tggtcaggcc gcgccacgag
     5521 ccagaggtgg gtgtgcatga aggtcttcat atcggcggac atcgagggtg tcaatggagt
     5581 catgggggag actcattggt catcgcaggg cagcgcctac gaccatgccc gggcgtggat
     5641 ggccgaggag gtcaacgcgg ccgtgcgcgg tgcgtgtgcc ggcggcgcca gcgagatcgt
     5701 ggtcaaggac tcgcacgggc ctggaaccaa catcctcgct caccagctcg acccgcgcgc
     5761 ccagctgatc agcggctggg gtccgcttca cagcatggtc gaaggagtgg acgagagctt
     5821 cggggcggct ttccttgtgg gctaccacgc caaggtgggc acggagcgcg gcgtgctatc
     5881 gcacactatt ggcagcacgg tgcgcgggct tcggctcaac ggcgaagcca tcggagagac
     5941 ggcactgagc gcgctgtact gcgggcacca cggcgtgccc gtggtccttg tcacggggga
     6001 cgacgaactc gcggccgagg cgcagcactt cctgcctgga gttgccacgg cggtggtgaa
     6061 atgggggctc aaccggggat cggcccgaat gctgccactg gctcaaggcc gagcacggat
     6121 cgaggaggcg gccgccgagg ccacggctcg agccggaagc atcgagccat ttcgcccgtc
     6181 actacccctc ggtctcgagc tcgatgtgcc gtcggcggcc gcagcggccc tctgcgctct
     6241 cgtgcccggt gtcgagcgga cggccgactg cacggtcgcg tgctcttgtg gcgacgccgt
     6301 ggagtacgtc aggatgttcc gagtgctgct gaacctggca tcgaccgcct agcggtgggt
     6361 gaggagagga atgcgttcga tgacaaccgt cgtgctccgc ctgttcctcg cttccgtggt
     6421 gatctgcggt gtcgcgtccg ctcaggaggc atcgttggcg cagaggctcg gcttcaccga
     6481 tgaggacatc gtgctgatca tcaacggcga tgatctcggc atgtgccatt cggccaacga
     6541 ggccgtcttc gacgcgctcg gccgcggatg cctgacgacg gcgacgctga tggtgccctg
     6601 cccgtgggct atcgaggtgg cccatcgctg gaaggagcaa cctgacctcg acatcggcct
     6661 gcacctgacc catacggccg agtggaggca ctatcgctgg gggccggtgg cctcgcgtca
     6721 cctggtgccg gggctcatcg atgccgaagg
//
LOCUS       DAOSPL010000036         6732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_11411_length_6732_cov_3.232589, whole genome shotgun sequence.
ACCESSION   DAOSPL010000036 DAOSPL010000000
VERSION     DAOSPL010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11411_length_6732_cov_3.232589"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..1041)
                     /locus_tag="PLQ54_01250"
     CDS             complement(10..1041)
                     /locus_tag="PLQ54_01250"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOS91915.1"
                     /translation="MGHTTDEIQPARAEAEPATTPVARHAWLPMTLRGLLALVVLAAL
                     GHYVAGLWASEQRQGLTIRWPWALASVVLYSVGMCQGAAFWRACMADMGLRAPWHKAM
                     AAYWLSQVAKYVPGKAWVVVVRCSAITREGRDLTPAAAATFYETLLVMGVGSAVSLIC
                     FAVAGGDGSNILVAASAGCVLLFAGVLVPAVFRRLMRTMLLPFPQARGQEVLHCSGRT
                     IAWGVVRGALLWVLLGMSTLACVNATAPEVAPVGLLLPLTGGMALATVGGFVVIFMPG
                     GLGVREGLLIAAATPVIGKAPAGVAALVVRLLWVFAEVATALGMLAWQKVVEHRRGRV
                     GASASSSPR"
     gene            complement(1066..3087)
                     /locus_tag="PLQ54_01255"
     CDS             complement(1066..3087)
                     /locus_tag="PLQ54_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91916.1"
                     /translation="MEAPDPVAEGVQKPASPPRQTPAAREGLRAGTLATALVLVTAVA
                     VGGAVSALVNYDLIGLGHLPRCAVFAVVILLVANALCRRFGRGHPFGRGQLAFVYIAV
                     LVMAGIPGQQLVTYLYVGMIGSQYHAAGSSSEYRTEVLPYLKPWMVPVQDPADPFNAE
                     QPVISWAMHGMPWGASVPWRPWVRPLLLWTPYLLALLFLQVCLAALLRRRWDEERLTY
                     PLAQAPIELMTYASEKAALPKLMTSPLFWGMALIPAVVHTKNALAMLYPYAFTRIDLN
                     KNVGVIFGEAPWSNFDYLPIQVYFETIGATYLIPVGTGFSLWFFWLVRRFICVYRDTQ
                     GLIDHQAYLQQQGIGAYLLLAALCVWGARKSLAEAFQGAVSRGNHARGDSSHEPLAPR
                     WAVMGVVLSLATTLAWGVAAGGTPLSIGLMMALHVAGMIVLSRLVAECGLFCVWTPLC
                     PPQEMVARTFGAFRPLSPQATTALCYMGWKVQDTASMTMANVMQGFKVAEMASIRPSS
                     ALWLMVGALVLGVFASHPPSLKAIYENGYYALGWWPRGTTDAVPASIHNLTKGLTAFK
                     GENATAVAHGALVVTALHLLRTQFHRFPLQPFAYAAALGPQFMMDRYGFSIFIGWCFK
                     HFVLKYGGVATHSKLRPAAFGLIAGNAAILLLWTLYFYFHPVEGVLVTE"
     gene            complement(3506..4705)
                     /locus_tag="PLQ54_01260"
     CDS             complement(3506..4705)
                     /locus_tag="PLQ54_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS91917.1"
                     /translation="MRDSGSGFHPMTRRRFLTLGAFGLSAAAQAAVRRERRGEMAYRR
                     LGRTDLMVSEVSLGGSPPPDEPLFSQAMDLGINYVDTSPAYGEGSCERLVGKMIRDRR
                     DRFYVTGKVFAQPGASAAADYVASVEATLERMRTDYLDILQIHGAERATEHQDAEILE
                     AFRTLKEQGKIRFAGLTCHTDPVAVLEPAIRSGHYDVVTIAYNAYLDAQPSGKGTDYL
                     GQCGIAQLIALANQHDVGVIAMKTLKCEKVQDLSAFTGEGSSMAQAMLKWALSNPGVS
                     AVITEMLTEEQLLEDAAVSAQELTEPEARALRRYVAHTSPAICRSCGACARHCPASIR
                     IPDVLRFASYALGQGKPARGRALYAGLPREQSAVACQRCGTCREACPFGLDTPALLAQ
                     AHRVLVG"
     gene            4882..5778
                     /locus_tag="PLQ54_01265"
     CDS             4882..5778
                     /locus_tag="PLQ54_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91918.1"
                     /translation="MTRPGFARAIPVWGFMCGCLWWGCALAQEEAPSPRPPSDTVLLR
                     YKPAVGDAVEYHFDFASRFDIEDRIGIRYDRTDVTMRARVSQKVTEVRPDGTAVVDTT
                     SQLVEMTPKPDDEFVPIRNLITMDNQRRLLEIADAPGENAVETDAGWRDLVVQAMNSL
                     SFPDEPIRIGDRWERTHTLTMPETGTVTKMEVKSQLTDRVPDGDRWVSLVSAEAQIPL
                     DLRTGDTRVAGNIGLHLRLEVFEDDGGLRYVRGWGRGSAKPDLKVRVAKIRILVDDMH
                     FQRFMPDGSLLPSVRGENAGKE"
     gene            5992..>6732
                     /gene="glgP"
                     /locus_tag="PLQ54_01270"
     CDS             5992..>6732
                     /gene="glgP"
                     /locus_tag="PLQ54_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOS91919.1"
                     /translation="MPKAYRTVMVSPSLPSRVEPLRGLAFDLSWTWRQDAIELFQRMD
                     RGAWENTHHNPVAMLGEIPQERLEALADDESFLEHLGRVVAETHAYLLEQTWFDRHSP
                     LAPGHRIGYFSLEFGISECLPIYSGGLAVLAGDHLKSASDLGVPVAAVGLLYQEGYFR
                     QYLNIDGWQQEEYPENDFANLPVQPALGPDGTQRRITVQVGDHDVQIALWRAMVGRVS
                     LYLLDTNLPENNPYDQEITARLYGGDREV"
BASE COUNT         1182 a         2280 c         2269 g         1001 t
ORIGIN      
        1 cctcttcgtt cagcgcgggg acgacgatgc tgacgcgccg acccggccgc gacggtgttc
       61 cactaccttc tgccatgcga gcatccctag ggccgtcgcc acctccgcga atacccagag
      121 gaggcggacg acaagagcgg ccacgcctgc cggggccttg ccaatgaccg gcgtggcggc
      181 cgcgatcagc agcccctcgc gcacgccgag gccaccgggc ataaagataa ccacaaaccc
      241 acccaccgtg gctagagcca tcccaccggt caggggcagc agcagaccca ccggggcgac
      301 ctccggcgcc gtggcattca cgcaggccag tgtgctcatg cccagcagca cccacagcag
      361 ggcgcccctc accacgcccc acgcgatggt gcgacccgag cagtgcagga cctcctgccc
      421 gcgcgcctgc gggaagggca ggagcatggt gcgcatcaac cgccggaaca cagcgggcac
      481 cagaacgccg gcgaacagaa ggacgcaccc ggccgaagcc gccaccagga tgttcgagcc
      541 gtcgccgccg gcgaccgcga agcagatcag cgatacagcc gaccccaccc ccatgaccag
      601 gagggtctcg tagaaagtgg ccgccgccgc gggagtgagg tcccgcccct cacgggtgat
      661 ggcgctacag cgcaccacca ctacccacgc cttcccgggg acgtacttag ccacctggct
      721 cagccagtag gccgccatgg ccttgtgcca cggtgcccgc aggcccatgt cggccatgca
      781 ggcgcgccag aaggcggcgc cctggcacat accgaccgag tagagcacca cgctggcgag
      841 ggcccacggc cagcggattg tcaggccttg ccgctgctcg ctggcccaga gcccggccac
      901 gtagtggccc agcgccgcca gcactaccag ggcgagcaga ccccggaggg tcatcggcag
      961 ccatgcgtgg cgggccacag gcgtcgtcgc gggttccgcc tcggcacggg ccggctggat
     1021 ctcgtctgtg gtatggccca acccatgctc cgctccaggc gcgctctact cggtcaccag
     1081 cacgccttcc accgggtgga agtagaagta gagtgtccag agtagcagaa tcgccgcatt
     1141 gcccgcgatg agtccaaagg ccgccggacg gagcttgctg tgggtcgcca cgcccccgta
     1201 cttgagtacg aagtgcttga agcaccaccc gatgaagatc gagaagccat agcggtccat
     1261 catgaactgc ggcccgagtg ccgccgcgta ggcgaaaggc tgcagcggga aacggtggaa
     1321 ctgcgtgcgc agcaggtgaa gcgccgtcac caccaacgct ccgtgagcca ctgccgtggc
     1381 gttctcgccc ttgaaagccg tcagaccctt ggtcaggttg tgaatgctgg ccggcaccgc
     1441 atcggtggtc ccgcgcggcc accagccgag ggcgtagtag ccattctcgt agatcgcctt
     1501 cagtgacgga gggtgtgagg cgaacacgcc caggaccaag gcccccacca tcagccacag
     1561 ggcgctgctc gggcgaatcg acgccatctc ggccaccttg aagccctgca tcacgttggc
     1621 catggtcatc gacgccgtgt cctggacttt ccagcccatg tagcacaggg ccgtggtcgc
     1681 ctgcggcgag agcgggcgga aggcaccgaa cgtcctcgcc accatctcct gcggtgggca
     1741 gaggggtgtc catacacaaa acaggccaca ctccgccacc aggcgggaga gcacgatcat
     1801 ccccgccacg tgcagggcca tcatgaggcc gatcgacagg ggggttcccc ccgccgccac
     1861 gccccacgcg agcgtggtag ccagagacag cacaacgccc atcaccgccc agcgcggcgc
     1921 caagggttcg tgcgatgagt cccccctggc atggttccca cgcgacacgg cgccctggaa
     1981 agcctcggcc agcgacttcc gtgcacccca gacgcacaga gccgccaaca gcaggtacgc
     2041 tccaatgcct tgctgctgga ggtatgcctg gtgatcgatc aacccctggg tgtcgcggta
     2101 gacgcagatg aaccgccgca ccagccagaa gaaccataag ctgaacccgg ttcccacggg
     2161 gatgagatac gtcgccccga tggtctcgaa gtagacctgg atcggcaggt agtcgaagtt
     2221 cgaccacggg gcttcgccga agatgacgcc gacgttcttg ttcaggtcga tgcgcgtaaa
     2281 ggcgtacggg tacagcatcg ccagcgcgtt cttggtgtgc accacagcgg ggatcagcgc
     2341 catgccccag aacagggggc tcgtcatcag cttcggcagg gccgctttct ccgacgcgta
     2401 ggtcatcagc tcgatgggcg cttgcgccag aggataggtc aggcgctcct cgtcccatcg
     2461 ccgtcggagc agcgccgcca ggcacacttg caggaacagg agtgccagca gatagggggt
     2521 ccagagcagc aggggtcgga cccagggccg ccacgggacc gaggcccccc atggcatccc
     2581 gtgcatggcc caggagatca ccggctgctc ggcgttgaag gggtcggccg ggtcctgcac
     2641 ggggaccatc cagggtttga gatacggcag cacctcggtg cggtactcac tggacgaacc
     2701 ggccgcatgg tactgggagc cgatcatccc gacgtagagg taggtcacga gctgctggcc
     2761 cgggatgccg gccattacga gtacggcgat gtagacgaac gccagctgcc cgcggccgaa
     2821 ggggtgaccc cgcccgaacc ggcggcagag cgcgttagct accagcagga tgacgacggc
     2881 gaacacggcg cagcgcggca ggtgcccgag gccgatgagg tcgtagttca ccagagcgga
     2941 cacggcgccg cccactgcga cggcggtgac cagtacgaga gcagtggcga gagtgccggc
     3001 gcgcagaccc tcgcgcgccg cgggggtctg cctcggcggc gatgctggct tctggactcc
     3061 ctcagccacc ggatccggcg cttccatagg cccctcgtta gccgcaatcc tgagcgcgcc
     3121 gattatcgca gccccgcgcc ccggtggcaa cggcagacac atgtgcgggg aaggcggcct
     3181 gcgacggccc cggcaacgtg ggcgcaatag gactagccca ccgtgccgcg ccgggccacc
     3241 atggggacag agccctgcgc gtaggctacc gtctctgccg ggaggccgtg ccgcggggct
     3301 caatccccct gttgcggagg ggcgccatgg ggggaaccac gcgcgctcca gcgtggtggg
     3361 tccccaacgc gcccgatcct ggggctaccg acccgggccg attgggggcc cctcgccctg
     3421 cgggcgcgtc tcccatctgg cccctccacg gaacgtgctc ggtgcgaggg ttcggggccc
     3481 gccgttctac cgccgggccc cgcccctacc ccaccaacac tcgatgggcc tgggccagga
     3541 gagccggggt gtcgagcccg aaggggcagg cctcacggca ggtgccgcac cgctggcagg
     3601 ccactgccga ctgctcgcgc gggagccccg cgtacagtgc ccgcccccgg gcgggtttgc
     3661 cctggcccaa ggcgtacgag gcgaagcgga gcacatcggg gatgcggatg ctcgcggggc
     3721 agtgccgggc gcatgccccg cagctgcggc agatggcagg cgacgtgtgg gccacatagc
     3781 gccgcagggc ccgcgcctcg ggctccgtca gctcctgagc cgagacggcg gcgtcttcga
     3841 gaagttgctc ttcggtcagc atttccgtga tgacggcgga cacgccaggg tttgagagtg
     3901 cccacttgag catggcctgg gccatggaag agccctcgcc agtgaaagcc gacagatctt
     3961 gcaccttctc gcacttgagg gtcttcatgg cgatgacacc gacatcgtgc tggttggcca
     4021 gcgcgatgag ctgggcgatg ccgcattggc cgaggtagtc cgtgcccttc cccgacggct
     4081 gggcatcgag gtaggcgttg taggcgatgg tgaccacatc gtaatgcccg ctcctgatcg
     4141 ctggctcgag cacggccacg gggtcggtat ggcaggtcag gccggcaaac cggatcttgc
     4201 cctgctcctt gagcgtccgg aaggcctcaa ggatctcggc gtcctggtgt tcggtagcac
     4261 gctcggcgcc gtggatctga aggatgtcca ggtagtccgt tctcatgcgc tcgagcgtgg
     4321 cctcgacgct ggccacatag tcggccgcgg cactcgcccc gggctgggcg aaaaccttgc
     4381 cggtgacgta gaaccggtcg cggcggtccc ggatcatctt gccgaccagc cgctcgcagc
     4441 ttccctcacc gtaggcgggt gaggtgtcga cgtagttgat gcccaggtcc atggcctggc
     4501 tgaagagggg ctcgtcgggc ggcgggctgc cccccaggct cacctcggac accatgaggt
     4561 cggtgcggcc cagacgccgg taagccatct cgccgcggcg ctcgcggcgg acggcggcct
     4621 gggcggcggc gctcagaccg aaggcgccca acgtcaggaa ccgccggcgt gtcatcggat
     4681 ggaaaccaga acccgagtcg cgcatcagta tccctcggct atgcgcccgg cggggagcgg
     4741 ggcgacggaa cccgggcgac gggactgcgt ctggcgtact gtggggatcc atgaccgcca
     4801 ccccactcta tggtatcctc tgcgcgagtc gaggagacag gcgtttgtct gctcttcgag
     4861 acggaaggag cggcgagacc aatgacacga ccgggtttcg cgcgggcaat cccagtgtgg
     4921 ggcttcatgt gcggctgcct ctggtggggg tgcgccctcg cccaggaaga agcgccctcg
     4981 ccccgtcctc cgtctgatac cgtgcttctg cggtacaagc cggcggtggg cgatgcggtc
     5041 gagtaccact tcgactttgc ctcgcgcttc gacatcgagg accgtatcgg cattcgctac
     5101 gatcggaccg acgtcacgat gcgggcgcgg gtgagccaga aggtcaccga agtgcgtccc
     5161 gacgggaccg cggtggtgga caccacctcg cagctggtgg agatgacccc gaagcccgac
     5221 gacgagttcg tgcccatacg taacctgatc accatggata accagcgccg ccttctcgag
     5281 atcgcggacg ccccagggga gaacgcggtg gagaccgatg ccggctggcg ggacctggtg
     5341 gtgcaggcca tgaacagcct cagtttcccc gacgagccca ttcggatcgg cgaccggtgg
     5401 gagcgcacgc acaccctcac tatgcccgag acgggcacgg tgaccaagat ggaggtcaag
     5461 agccaactca ccgaccgagt gcctgacggc gaccgatggg tcagcctggt ctcggcggag
     5521 gcccagatcc cgctggacct gcggaccggc gatacgcggg tcgcggggaa catcggtctg
     5581 cacctgcggc tcgaggtttt cgaggatgac ggcgggctcc gctacgtccg gggctggggc
     5641 cgtggttccg ccaagcccga cctgaaggtg cgagtcgcca agataaggat tctggttgac
     5701 gacatgcact tccagcgctt catgcccgac ggtagcctac ttccctccgt gcgaggagag
     5761 aacgcgggca aagaataggc aaagacttac tccgaaatac cccaacgccc cactccggcc
     5821 aaactcgtcc tgcccgcgca caggagactg cgcgcctgag cggaatcagg atcttggcgc
     5881 gtgtgtcgtt tcgctaccga ccgaacgcac tgcgtgccgc gcatctggcc ttggctgggg
     5941 ttgacgtgct agggacggcc cacccgcgtg ggcccatggg aggcgcattg catgccaaag
     6001 gcgtaccgga ccgtcatggt ctcgccctcg ctaccgtcca gagtggagcc cctccgtggg
     6061 ctcgcgttcg acctctcgtg gacgtggcgg caggatgcca tcgagctgtt ccagcgcatg
     6121 gatcgtgggg cctgggagaa cacgcaccat aacccggtgg cgatgctggg tgagatccca
     6181 caggaacggc tcgaggcgct ggcggacgat gagagcttcc tcgaacacct gggccgcgtg
     6241 gtggccgaga ctcacgcgta cctgctcgaa cagacctggt tcgaccgcca cagccctctg
     6301 gcccccggcc atcggatcgg ctacttcagc ctggagttcg gcatctccga gtgtctgccc
     6361 atctactcgg gtggtctggc agtccttgcc ggcgatcacc tcaagagtgc gagcgacctg
     6421 ggcgtgcccg tggcagcggt gggtctgctc tatcaggagg gctacttccg ccagtacctc
     6481 aacatcgatg gctggcagca ggaggagtac cccgagaacg acttcgcgaa cctgcccgtc
     6541 cagcccgctc ttgggcccga tggcacgcag cgccggatca cggtgcaggt gggcgatcac
     6601 gatgtccaga ttgccctgtg gcgggccatg gttggccgcg tatcgctgta cttgctggac
     6661 acgaaccttc ccgagaacaa cccgtacgac caggagatca cggcccggct gtacgggggc
     6721 gaccgcgagg tg
//
LOCUS       DAOSPL010000037         6699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_11484_length_6699_cov_3.337899, whole genome shotgun sequence.
ACCESSION   DAOSPL010000037 DAOSPL010000000
VERSION     DAOSPL010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6699
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11484_length_6699_cov_3.337899"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..850
                     /locus_tag="PLQ54_01275"
     CDS             260..850
                     /locus_tag="PLQ54_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOS91920.1"
                     /translation="MVPAAALSGARAAGPGSSEVLVLNQNFEPLNVTNLRRAIALIVL
                     EKAEPLETNGRVLHSEHEALVAPSVVRLRHYVRRPVPELRLTRASVFARDGHTCLYCG
                     RHGGGLTIDHVIPRARGGAYDWTNLVTCCLKCNNKKGNRTPQEAGMRLLARPLRPRYT
                     PYISLPKFLEAYRADRWTKYLQPFIDYAGLGELRDA"
     gene            1069..2292
                     /gene="ftsZ"
                     /locus_tag="PLQ54_01280"
     CDS             1069..2292
                     /gene="ftsZ"
                     /locus_tag="PLQ54_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003728828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOS91921.1"
                     /translation="MLAPETHNYAKIRVVGVGGGGGNAITRMIESGVTGVEFLAINTD
                     AQALDRIQADNKLQIGVSLTKGLGAGGDPQIGRAAAEESKQEIMMALDGSDMVFLTCG
                     LGGGTGTGASPVVAEIARSLGALTVAICTRPFQIEGPRRKANADDGFNHLKENVDAIV
                     VIPNDRLISVVEKRTTLAEAFQSADDILRQGVQGISEVIVIPGLINLDFADVRAVLAN
                     AGTALMGIGVGQGEHRAPDAAKIAVSSPLLEQTIDGAEAILFNITGPADLGLNELQEA
                     AEIITSSTKQQNPNVLMGAVIDERMQNEVRITVVATGFDRKPSVPTASTPRHGTQEGR
                     LPSAELSVPQRASLLRQAYSGQPPTVSVQLPDDSRAKPAGQLQDDTRRGPAFPSVAQP
                     VQDDDLDIPAFLRRK"
     gene            complement(2374..4050)
                     /locus_tag="PLQ54_01285"
     CDS             complement(2374..4050)
                     /locus_tag="PLQ54_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOS91922.1"
                     /translation="MTFLDAIVVLAYLLGIAVIGVYQSVRVRSSGDYFAGGRKFSRWL
                     MVMHALGTGTHADDPVGVAGAAKQHGIAGIWYTYVYLFATPFYWIMAPFFRRSRYITT
                     ADFFRDRFSPGLAVLYTIMGMLTFAVNTGTLLRGTGTIVTSVTQDRVPEWLGIGAMTV
                     VFVAYGLAGGLVATVVTESIQGVLIVVMSLLLIPFGLARVGGFGALHQLLDPSRFSLA
                     ATEEVTWQWAAGCTVAMIIGIVAQPHIMEVCSTGKTEFEGRVGFTYGNFVKRFCAMGW
                     AFTGLIVTAMVAAGMLPELGQREDAFGVAIRELLPSGVTGLMFAAILAAQMSTLSAFM
                     VAGSALFSRNIYREYFRRDATDAQVLAIGRWAGLIVVLLGVMMAAVVRSVADALTIFW
                     GLATLTGLFMWAGVLWRRTNAAGAWASFIVMALIWLALGKGGQLAATALGDAAPTWLG
                     LYADRGHMHRLLIAYLLPGTVVLVAVSLLTRPKPQAELDRFHTLIRTPVGREEELEKA
                     GIEAVYAGASEGHPWELNHPKLVNWAGFGLALAFCFVILAILWGLTRIGA"
     gene            4242..5207
                     /locus_tag="PLQ54_01290"
     CDS             4242..5207
                     /locus_tag="PLQ54_01290"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOS91923.1"
                     /translation="MSYSIRVAVTGISGHLGSHLAPLLAEDGFEVVGLDVASPRQPLP
                     DRSRFTLTDLCDEDAVRNTLAGADLVVHCASVHPWKPYSDSQYLDCNVKGTWVLYTAA
                     ASLGINRIVLTSSIAAVGYGVGAAWPVTEDVESPLGDLYGYTKHAQEDIARVFADAGK
                     VRTLALRPPAFMPRAELETGFSLTGPYAVVEDVAGAHLEAVRVLSGRKPAGAEMAPFE
                     AVFVTNQLPYGPDDAALMATEGSAKPLVRRHWPEAYEWLIARGYEGTWVPAAYDLSKA
                     RRLLGWSPKRNFEQWFAEAKGKLQAPIPPAGSAHRPAAAEPVRHR"
     gene            complement(5146..5871)
                     /locus_tag="PLQ54_01295"
     CDS             complement(5146..5871)
                     /locus_tag="PLQ54_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91924.1"
                     /translation="MQAVRDSGVRCVHDIPMVKSLPPFARVRAAFGTAEFGHVVAYHH
                     VFGMTFPMGFPVRDLWPERFDPPAISGGGEMTNMGCYAIDYMVTLLGSPRAVQAKRQS
                     FWQEYAESPVGDFGQIVCDYGRFYAVLTVGKQKLTAPLAPTMNTVSIVFQNANLLVDP
                     VSDTITVNGIRRSTAEFVGATAVEGSLDQLIRCIETGVEPLDTVEQGRRGMEVLMAAY
                     RSAISGGERVLLPLGDGLNPLVG"
     gene            complement(6116..>6699)
                     /locus_tag="PLQ54_01300"
     CDS             complement(6116..>6699)
                     /locus_tag="PLQ54_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS91925.1"
                     /translation="LMLDLATNEPRLHELIARVMHHNEVIVDQYLSMGVDVMGFAEDL
                     GTQTASLVSPATFREWFTPQYRRLMAPCKPVGTLVHLHTDGYVMELSGEFITAGVDIL
                     NVQDLCNGTDNLARELKGRVCIDLDVDRQSIVPFGTRSEIRALIEEEVRTLGSPRGGL
                     SMVCGIYPPTPPENVDAVCCAMEEFRSYWADRR"
BASE COUNT         1193 a         2277 c         2179 g         1050 t
ORIGIN      
        1 ctcgggccgg tcaggggccc cgcgccctct gggcgcaccc ctgtgcggcc cctccccggt
       61 tgctcggcgg cctgccggaa cccggcgaaa gctgctgaga ccctatccgg cctgagggtc
      121 gccgcgcttg ccctaccccg ccacgtggca cgcgacgaag tgccccgggg ccggctcgcg
      181 ccactggggc tgctcgcgcc ggcgggtccg tttgacggca cggtgggtcg tggctagaat
      241 ggcgtcgttc ggggagggga tggttccagc cgctgccttg agtggggcca gggccgctgg
      301 ccccggatcg agtgaggttt tggttctcaa ccagaacttc gaaccgctga atgtcaccaa
      361 cctgcggcgc gccattgctc tgatcgtcct cgagaaggcg gagccgctgg agaccaacgg
      421 tcgggtgctt cactcggagc acgaggctct cgttgcgccg tcggtggtac gcctgcgcca
      481 ctacgttcgc cgtccggttc cagagcttcg cctcacgcgc gccagtgtgt ttgcccgcga
      541 tgggcacacc tgcctctact gcggccgtca tggcggcggt ctcaccatcg accatgtgat
      601 ccctcgggct cgcggtggtg cctacgactg gaccaatctg gttacctgct gcctcaagtg
      661 caacaacaag aagggcaatc ggacgccaca agaggccggc atgaggcttc tcgcgcgacc
      721 gctgcgcccg cgctacacac cttacatcag cctgccgaag ttcctcgagg cctatcgggc
      781 cgaccggtgg accaagtacc tccagccgtt tattgactat gcgggtctcg gcgagttgag
      841 ggacgcgtag ctccgggagc cccatgccgc cgacggtttg ccttgcctct ccgggggctc
      901 ctataatgtt tccatgagtt cgccggcccc gcgaaccgct gcacgggacg gacggtttgg
      961 gttgcggggg tagaccgcgc aggtgggcgc ggttgggctg ggccttcgtt cagcgtaggc
     1021 ccctgtggtg tgtaccggcc gcagacagcg ggttggaggc cttcaacgat gcttgctccc
     1081 gaaacgcaca actacgccaa gatccgcgtg gtgggtgtcg gcggcggcgg tggcaacgcc
     1141 atcacgcgga tgatcgaatc gggcgtaacc ggcgtcgagt tcctggcgat caacaccgac
     1201 gcccaagcac tcgaccggat ccaagcggac aacaagctcc agatcggcgt cagcctgacc
     1261 aaaggcctgg gcgcgggagg cgatccccag atcggccgcg cggcggcgga ggagagcaag
     1321 caggagatca tgatggccct ggacgggtcg gacatggtct tcctcacctg cggcctgggc
     1381 ggcggaaccg gcacgggcgc ttcgcccgtt gtcgccgaga tcgcgcggag cctgggcgcg
     1441 ctgactgtgg ccatctgcac tcgaccgttc cagatcgagg gcccgcgccg caaggcgaac
     1501 gccgacgacg gcttcaacca cctcaaagag aacgtggatg ccatcgtcgt gatccccaac
     1561 gaccggctga tttccgtggt cgagaagcgc accacactgg ccgaggcctt tcagtcggcc
     1621 gatgacattc tccggcaagg ggtccagggc atctccgagg tcatcgtgat ccccggtctc
     1681 atcaacctcg acttcgccga cgtccgggcc gttctggcga acgccggcac ggcgctgatg
     1741 ggcatcggcg tgggccaggg cgaacaccgc gctccggatg ccgccaagat cgccgtctcg
     1801 agcccgctgc tcgaacagac catcgatggc gcggaagcga ttctcttcaa catcacgggc
     1861 ccggccgatc tgggactcaa cgagctccag gaagccgccg agatcatcac cagctccacg
     1921 aagcagcaga accccaacgt gctgatggga gccgtgatcg acgaacggat gcagaacgag
     1981 gtgcggatca ccgtagttgc caccggcttt gaccgcaagc cctccgtgcc caccgcctcg
     2041 acgccgcggc acgggactca ggagggccgc ctcccctcgg cggagctgtc cgtacctcag
     2101 cgcgcatcgc tcctgcgcca ggcctacagc gggcagcccc ctacggtatc ggttcagctg
     2161 cccgatgaca gtcgcgcgaa gccagcaggg caactccagg atgacacgcg gcgtggaccg
     2221 gcgttccctt cggttgccca gccggttcag gatgacgacc tggacatccc cgcgttcctg
     2281 cggaggaaat agcggggcaa tggtctagat gggctcgcac cccacgctcc gagtgccggt
     2341 ctgcgtatgt cgcgtgcccc cgcggcgccc gaactaggcc ccaattcgcg tgaggcccca
     2401 cagaatggcc aggatcacga agcagaaggc cagagccagg ccgaagccgg cccagttcac
     2461 gagcttcgga tggttcagct cccacggatg cccctcagac gctccggcat agactgcctc
     2521 gatcccggcc ttctcgagct cttcctcgcg cccgacgggt gtgcggatga gtgtatggaa
     2581 ccgatcgagt tccgcttgcg gctttgggcg cgtcaacaga ctgacggcaa ccagcaccac
     2641 cgtcccgggc aaaagatagg caatcaggag ccggtgcata tggccccggt cggcgtacag
     2701 gcccagccac gtgggcgcgg cgtccccgag ggctgtggcc gccagttggc cgcctttacc
     2761 cagcgccagc cagatgagcg ccatgacgat gaacgaagcc catgcccccg cagcattggt
     2821 ccgccgccag agcacgccgg cccacatgaa caggccggtc agtgtcgcca gaccccagaa
     2881 gatggtgagg gcgtcggcca cgctccggac gaccgcggcc atcatgacgc ccagcaacac
     2941 gacgatgagc ccggcccagc gcccgatggc cagtacctgg gcatccgtgg cgtccctgcg
     3001 gaagtactcg cggtagatgt tgcgcgagaa cagggccgag cccgccacca tgaacgcact
     3061 gagcgtcgac atctgagccg ccaggatggc ggcgaacatg aggccagtga cgcccgaggg
     3121 cagtagttcg cggatggcga ctccgaacgc gtcttcgcgc tgacccagct cgggcaacat
     3181 ccccgcggcc accatggccg tgacgatcag gcccgtgaat gcccaaccca tggcacagaa
     3241 gcgcttcacg aagttcccgt acgtgaagcc cacgcggccc tcgaactccg tcttgccggt
     3301 cgagcagacc tccatgatgt ggggttgagc cacaatgccg atgatcatcg ccactgtaca
     3361 cccagcggcc cactgccatg tgacctcctc ggtcgccgcc agggaaaagc gcgagggatc
     3421 caacagctgg tgaagcgccc cgaagcctcc cactctcgcc agtccaaagg ggatcaacag
     3481 gagcgacatc acgacgatga gcacgccctg gatcgactcg gtgaccacgg tggccacgag
     3541 gccgccggcg aggccgtagg cgacgaagac cacggtcatg gcgccgatgc caagccactc
     3601 gggcacacgg tcttgtgtga ccgacgtgac gatggtgccc gtccccctca acagcgtacc
     3661 ggtgttcaca gcgaatgtca gcatccccat gatcgtatag agcacggcca gaccagggct
     3721 gaagcggtcg cggaagaagt cggccgtcgt gatgtagcgc gaccggcgga agaagggggc
     3781 catgatccag tagaaggggg tggcgaagag ataaacgtag gtgtaccaaa tgcccgcgat
     3841 gccatgctgt ttggcggcac cggccactcc aacggggtca tcggcatggg tgcccgtgcc
     3901 gagcgcgtgc atgaccatca gccagcggct gaacttgcgc ccgcccgcga agtagtcgcc
     3961 cgaggacctc actctgacgc tctggtagac gccaatgaca gcgatgccca acagataggc
     4021 cagtaccaca atcgcatcca ggaatgtcac gcgagcaacg ccctccctga gccgcgctgc
     4081 gaaacgtccc caggtttcgc cggtggtcca aggacccctg cacgacggga agcgccgtgg
     4141 tcgcaccccg gctcacccga gagacgagca ggcgcgccgg cgtgagcgaa gaagggtacc
     4201 tcgaggggcc ttgcagcgcc ccatggaccg gagggccgcg catgtcctat tccattcgcg
     4261 ttgcagtgac cggcatctcg ggccacctgg gctcgcactt ggcccccctt ctggccgagg
     4321 acggcttcga ggtggtgggg ctagacgttg ctagcccgcg ccagccgctg cccgatcggt
     4381 cgcgcttcac tctgacggac ctgtgtgacg aggacgccgt ccgcaacacg ctggccggag
     4441 ccgacctggt cgtgcactgc gcgtcggtcc atccgtggaa gccctactca gactcgcagt
     4501 acctggactg caacgtgaag ggcacgtggg tgctctacac agcggccgca tcccttggca
     4561 tcaaccggat cgtgctcacc agcagcatcg cggcggtcgg ctacggtgtc ggggcggcct
     4621 ggccagtcac cgaggacgtc gagtcgcccc tgggtgacct ctacggctac acgaagcacg
     4681 cgcaggagga cattgcgcgg gttttcgccg acgccgggaa ggtgcgcacg ctggccctgc
     4741 gcccgccagc attcatgcca agagctgaac tcgagaccgg cttcagcttg accggcccct
     4801 atgcagtggt tgaggacgtc gccggggccc acctggaggc cgtgcgcgtc ctgtccggcc
     4861 gcaagccggc cggcgcggag atggcgccgt tcgaggccgt gttcgtgacg aaccagcttc
     4921 catatggccc ggacgacgct gccctcatgg ccaccgaggg ctcagcgaag cccctggtgc
     4981 gtcgccactg gccggaggcc tacgaatggc tcatcgcgcg gggatatgag ggcacgtggg
     5041 tgccggccgc ctacgaccta tcgaaagcgc ggaggctgct gggctggtcg ccgaagcgca
     5101 acttcgagca atggttcgcc gaggcgaagg ggaagctcca ggctcctatc ccaccagcgg
     5161 gttcagccca tcgcccagcg gcagcagaac ccgttcgcca ccgctgattg ccgagcggta
     5221 ggccgccatg agcacctcca taccccgccg cccctgctcc accgtatcga gcggctcaac
     5281 ccccgtctcg atgcaccgga tgagctggtc gagggaaccc tcgaccgctg tcgcgcccac
     5341 gaactcggcc gtggatctgc ggatgccgtt gaccgtgatc gtgtcactca cgggatcgac
     5401 cagcagattg gcattctgga acacgatgct gacggtgttc atggtcggtg ccagcggggc
     5461 agtcagtttc tgtttgccaa cggtgagcac cgcatagaag cggccatagt cgcacacgat
     5521 ctggccgaag tccccgacgg gcgactcggc gtactcctgc cagaagctct gccgcttggc
     5581 ctggacggcc ctggggctgc ccagcagcgt caccatgtag tcgatggcgt agcagcccat
     5641 gttggtcatc tcgcccccac cggagatggc aggcggatcg aaccgttcgg gccagaggtc
     5701 gcggacgggg aagcccatgg ggaaggtcat gccgaaaacg tgatggtagg cgaccacatg
     5761 cccgaactcg gccgtcccaa acgcggcgcg gactcgggcg aacggtggta gagacttcac
     5821 catcggaatg tcatgcacgc accggacgcc cgagtcgcgc acggcctgca cgatgcgccg
     5881 cgccgagggt ccttctcaaa gcccgccacg actcggactc gcgggtgggc agcgaggctg
     5941 ccggccaggc cggcaccgtg ggtgcagcag tcgccgagga tgcccacgcc atacgtgcgg
     6001 ttcggctcat ccaagggcca cccgtctcct tccacggcca gaacccggcc tccgaggacg
     6061 ctggccgcgg ccgagctcag aaacgctcgc cgcgtgcacg gctgcaggcc cgggttcatc
     6121 gccggtccgc ccagtagcta cggaactcct ccatggcgca acagacggca tcgacgttct
     6181 ccggcggcgt cggagggtag atgccgcaga ccatcgagag tccgcctcgg ggcgacccca
     6241 gcgttcgcac ctcttcttcg atgagcgccc gaatctcgct ccgcgtgccg aaggggacga
     6301 tgctctgccg gtcgacgtcg agatcgatgc agacgcggcc cttgagctcg cgcgccaggt
     6361 tgtccgtgcc attgcagagg tcctggacgt tcaggatgtc gaccccggca gtgatgaact
     6421 cgcctgacag ctccatcaca tacccatcgg tgtgcaggtg caccagcgta cccacgggct
     6481 tacacggggc catcaagcgc cggtactgcg gcgtgaacca ctctcggaac gtggctgggc
     6541 tcaccagcga ggccgtctgt gtgcccaggt cctcggcaaa ccccatcaca tcgacaccca
     6601 tcgagaggta ctgatcgacg atgacctcat tgtgatgcat cacccgggcg atcagttcgt
     6661 gcagccgtgg ttcgttcgtc gcgaggtcca gcatcaggt
//
LOCUS       DAOSPL010000038         6684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_11523_length_6684_cov_3.002564, whole genome shotgun sequence.
ACCESSION   DAOSPL010000038 DAOSPL010000000
VERSION     DAOSPL010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6684
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11523_length_6684_cov_3.002564"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /gene="gcvPA"
                     /locus_tag="PLQ54_01305"
     CDS             complement(<1..899)
                     /gene="gcvPA"
                     /locus_tag="PLQ54_01305"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOS91926.1"
                     /translation="MLEALGLESVEELFSPIPTELRLAGPLAIPGPLSDIELTQHCRE
                     LAFQDLDGTRAPCFAGTGVYDHFVPSTVAHLAYRGEFATAYTAYQPELSQGMLAALFE
                     FQTMVCELTAMDVSNSSVYDGGTALTEAAFVAAASTKRKRVLVASTVNPRYVECLRTG
                     AGAVLDIDMAPAEGGVSSPDAVATALTADHAALIVQHPNYFGCLEDVRALTEAAHAAG
                     ALLAVSFDPISLGLLEPPGAYGADIAVGEGGSMCGPMSLGGPGLGLFTCRKSLVRSLP
                     GRVVGKTVDHDGRVGYVNTLQTR"
     gene            complement(942..1325)
                     /gene="gcvH"
                     /locus_tag="PLQ54_01310"
     CDS             complement(942..1325)
                     /gene="gcvH"
                     /locus_tag="PLQ54_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOS91927.1"
                     /translation="MYPEDLKYTETHEWARVEGTEVTVGISSYAANELGDIVFVDLPE
                     VGAAVAVGESMGSIESVKAVEELRAPVTGTVSAVNEGLADTPEVVNSDPYGEGWMVKI
                     TLSDASELAKLMDAAAYKATIESGS"
     gene            complement(1351..2469)
                     /gene="gcvT"
                     /locus_tag="PLQ54_01315"
     CDS             complement(1351..2469)
                     /gene="gcvT"
                     /locus_tag="PLQ54_01315"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOS91928.1"
                     /translation="MKEVLKIPPGSRPTTLGPFHERLGARMVDFHGWWMPVQYESVLA
                     EARAVRETAGVFDVSHMGILRFGGPQVLDALQRLFTNDASLLAPGQAQYSLMLNDAGG
                     VVDDLIVYRDTDPGDQFLVVVNASNAERDVAWIADHLPPGAAMEDVSQSLDIIAVQGP
                     TSLELVRAIADGDPSQLASMSGAAMRVAGEKCFVARSGYTGEDGVEIFCGPEHSAKLF
                     QAVLQGNGRAGARPCGLGARDVLRMEAGLPLHGAELTPDCIPVGAGVSWAVKPAKGDF
                     IGGGPYRAALERGPSQIRRGLKLRGRRMAHPGARVLANQVAVGTITSGTFSPALGCAI
                     AQSYLVPEASLPGMVVQVEIGEGLYDADVIRLPFVRRT"
     gene            2773..3306
                     /locus_tag="PLQ54_01320"
     CDS             2773..3306
                     /locus_tag="PLQ54_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91929.1"
                     /translation="MRVQLFHPGTICLVALLLACTASFAVTPPTVTSTYAKVNTPQSG
                     NYQPNQTITGTTTHRAVFDTTNGEYQPFKLVIIWESYPSWAPCGAGTDDNLLYTYNQQ
                     SGITVYSSPNPVIYQWTTPGPGQYSYVANTIAIPYAASRTYGPPPPPPPPPDGRDYTQ
                     GDPVQGDPDVTQITVVP"
     gene            3343..4008
                     /locus_tag="PLQ54_01325"
     CDS             3343..4008
                     /locus_tag="PLQ54_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91930.1"
                     /translation="MVVRLLSLAAPVWGIVLGTVACPSCPAQPPAAPAPTAEAPAKTT
                     PPPAAYTVSFRIIKIDVAEGLDRREFADLPDSAFFITPESGDQFYDRVQLRQVFGEAI
                     RPGLVATVGPVYSVTVPPRAEPVAIRGVLMDGKTEWSVSLAVEPRSDDGMKVDLRSVW
                     LTKDLLAFETNPDPGKCEMYVGWPMPMWAGSGFSTNRPDREVGKNFERGYCVLVLVEP
                     AVM"
     gene            complement(4129..>6684)
                     /locus_tag="PLQ54_01330"
     CDS             complement(4129..>6684)
                     /locus_tag="PLQ54_01330"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOS91931.1"
                     /translation="ERLGSSQDWKTLSTWDVTGKLGPGANVVAAEATNADGPAAFVAG
                     LLIRYEDGQTQAITTSEGWRTAADAEGEWATAGYDDSAWVPPVVVAQWGAAPYGTPGQ
                     GGGGRNLRSQCFRKEFRLDQPVTSARAYVSGLGLYELRINGRKVGADELAPGWTLYDK
                     RVQYQTFDVTDLLRSGRNAVGAVVGNGWWSGRISWRTPPPGVRNVLRFVCELDVELAN
                     GEHVLVATDDSWRAHESPVLSDDLYDGETYDARLAMPGWDQPGFDDREWRPTAIIADD
                     IGRLVAQRGPAIRCTHALAPKAITEPSPGVFVFDFGQNLAGRERLRVKGPAGTAVRIR
                     FAEVLNPDGSIYTENYRSAKATDTYILRGDGEETWAPRFTYRGFRYAEVTGFPGKPAA
                     DALTAEAVHSDAPVIGRFECSEPLLNQIQHNIVWGQRSNMHSVPTDCPQRDERLGWTG
                     DAQAFAPTACWNMDMASFFAKWMLDLRDSQGPGGEVADIAPVGAPSAGSPAWADVITI
                     VPWNVFRFYGDARLVDTSFDSMRGWVEYMRRHAPNHLYEREGYGDWIAPVGTAKKPIG
                     TAYYYLSARRLAEMAAARGRDLDAKQYLALADDIARAFNEAYLAPDGNYPGGTQTANL
                     LPLAFGITPADRRAEVAAHIAQDVRGRNEHLSTGFLGTAFLLPVLSDMGYHDLAYRVA
                     TQRTYPSWGYMVEKGATTIWELWNSDTEGPGMNSRNHFALGGVGEWFYEYLAGIRPDA
                     DHPGFEHFTVWPRPVAGLAWAKADYNSPHGRIRSAWRLDGDRLVMDVEVPANTTATIH
                     VPTTDVSRIREGGRVSSPRAGLTYRGESSGAAIFDAVARRYHLETPWAPASSP"
BASE COUNT         1081 a         2242 c         2267 g         1094 t
ORIGIN      
        1 tcgcgggtct gaagcgtgtt cacgtagccg acacggccat cgtggtcgac ggtcttgccc
       61 accacacgtc ccggcaggga gcgtaccagg ctctttcggc acgtgaacag gcctagcccc
      121 ggccccccca ggctcatggg cccgcacatg gagccgcctt ctcccacggc gatatccgcg
      181 ccgtaggcac cggggggctc gagcaggccc agcgagatgg gatcgaagct caccgcgagc
      241 agggcgcccg ccgcatgggc tgcctccgtc agggctcgca cgtcctcgag gcagccgaag
      301 tagttcggat gctgaacgat gagggccgcg tggtccgcgg tcagggcagt cgccaccgcg
      361 tccggggagg agaccccacc ctcggcgggc gccatgtcga tatcgagcac ggcgccggcc
      421 ccggtgcgca aacactcgac atagcgcggg ttcaccgtcg aggccacgag aacgcgcttc
      481 cgcttggtcg aggccgcggc gacgaaggcg gcctcggtca gggccgtgcc gccatcgtag
      541 accgacgagt tcgacacatc catggccgtg agttcacaga ccatggtctg gaactcgaac
      601 aaggccgcca gcatgccctg gctgagttcg ggttggtagg cggtgtacgc cgtggcgaac
      661 tcgccgcggt aggcgagatg ggccaccgtc gagggcacaa agtggtcgta gacgccggtg
      721 cctgcgaagc agggagcccg cgttccgtcg agatcttgga acgcgagctc ccggcagtgc
      781 tgagtcagtt cgatatccga gagcggcccc gggatcgcga gcggtccggc gaggcgcagc
      841 tcggtaggta tgggggagaa cagctcttcg acggattcca ggccaagtgc ttcgagcatg
      901 gccatggcgt ccgcatccct ggacgatgcg agcgccatgg cttacgagcc gctttcgatc
      961 gtggccttgt aggccgccgc atccatgagc ttcgcgagtt cgctggcgtc cgatagggtg
     1021 atcttgacca tccagccctc gccgtacgga tcggagttca cgacctcggg cgtgtcggcc
     1081 aagccctcgt tgacggccga gacggtgccg gtcacggggg cgcgaagctc ttcgacggcc
     1141 ttgacggact cgatggaccc catggactcg ccgacggcca cggcggcgcc cacctcgggc
     1201 aggtcgacga acacgatgtc gcccagctca ttggcggcgt agctgctgat gcccacggtg
     1261 acctcggtgc cctcgacgcg ggcccactcg tgcgtttcgg tgtacttgag atcctcgggg
     1321 tacattcggt ctcctcctgt ttgtgccggt ctaggtgcgg cggacgaagg gcagccggat
     1381 cacatcggcg tcgtagaggc cctctccgat ctcgacttgg accaccatgc cggggaggct
     1441 ggcctcgggc accagatagg actgcgcgat agcgcaccca agagcgggtg agaatgttcc
     1501 gctggtgatt gtacccacgg cgacctggtt cgccaatacc cgggcgcccg ggtgtgccat
     1561 gcgccgcccg cggagcttga ggccgcgccg gatctggctc ggaccgcgct ccagtgcggc
     1621 tcggtacggc ccgccgccga tgaagtcgcc cttcgcgggt ttcaccgccc aggagacacc
     1681 ggcccccacg gggatgcagt ccggcgtgag ctcggcgccg tgaaggggta gtcccgcttc
     1741 cattcgcagc acgtctctgg cgccgagtcc gcacgggcgc gcgccggccc gcccattgcc
     1801 ctggagtacg gcctggaaca gcttggcgga gtgctcgggg ccgcagaaga tctcgacacc
     1861 gtcctcgccg gtgtagccgc tgcgtgccac gaagcacttc tcgccggcca cgcgcatcgc
     1921 agcgcccgac atcgatgcca gttggcttgg atccccgtcc gcgatggccc gcacgagctc
     1981 cagggatgtg gggccctgca ccgcgatgat atcgaggctc tgggacacgt cctccatggc
     2041 ggcaccggga gggaggtgat cggcgatcca ggccacgtcg cgctcggcgt tggaggcatt
     2101 gaccacgacg aggaactggt cgcccggatc cgtgtcgcgg tagacgatca ggtcatcgac
     2161 aacgccgccg gcgtcattga gcatgaggga atactgggcc tgaccggggg cgaggaggga
     2221 agcgtcgttg gtgaagaggc gttggagagc gtcgaggact tggggcccgc cgaagcggag
     2281 gatgcccatg tgactgacat cgaacacgcc ggccgtctca cgtaccgccc gggcctcagc
     2341 gagcacgctc tcgtactgga ccggcatcca ccagccgtgg aagtcgacca tccgcgcgcc
     2401 gagccgttcg tggaaggggc ccagcgtcgt cggccgagac ccggggggta tcttcaggac
     2461 ttccttcacg ctgcatcctc ctgttcgcgg cgagagtgcc atggccagcg caatggattg
     2521 ttcgggcgtc acgtcgcgat ctcctggcgg gaagccagag ccggagccag aggtccgagc
     2581 ggcggcgcgc ctgccgctcg gagatacctg acaagctatc tatgtcagaa aagggtaacg
     2641 cggcaattga cgtcgatgag cgatgtagat aagctctact cgttgcgggg gggggggctt
     2701 gtggcgtaca ggtcgcactc gcgtcgagcg gggtgtgcct gacgcacccc tcccggagga
     2761 ggtacgatga cgatgcgcgt acagctcttc caccccggga cgatctgtct agtggctctc
     2821 cttctggcct gcactgcgtc gttcgctgtc actccgccga ccgtcacatc gacgtatgcg
     2881 aaggtcaaca ctccccagag tggtaactac cagccgaacc agacaatcac aggcacaaca
     2941 acgcacagag ccgtgttcga cacgacgaat ggggagtacc aaccgttcaa gctcgtcatc
     3001 atctgggagt cgtacccttc gtgggccccg tgcggcgcgg ggacggacga caacctcctg
     3061 tacacctaca atcagcagtc gggcataacg gtgtactcgt cgcccaaccc cgtcatctac
     3121 cagtggacca ctccaggccc gggccagtac tcatacgtgg cgaacacgat cgccataccc
     3181 tacgcagcat cgcgaaccta cggtccccct cctccccctc ctccgccccc cgatgggcgc
     3241 gattacactc agggggaccc cgtgcaaggc gaccccgatg tgacccagat caccgttgtg
     3301 ccgtaggcca gtccgtcgcc taccggaaag ggcgtggtcc cgatggttgt ccgcctattg
     3361 tcactagcgg ccccggtctg gggcattgtg ctcggcacgg tggcctgccc ctcgtgcccc
     3421 gctcagccgc cggcggcgcc agcgccaacc gccgaggcgc cggccaagac gactccgcca
     3481 cccgcagcct acacagtctc gttccgcatc atcaagatcg acgtggcaga aggtctcgat
     3541 cggcgcgagt tcgccgatct tccagatagc gcgttcttca ttactcccga gagcggcgac
     3601 cagttctacg accgtgtgca gctccgtcag gtgttcggcg aagccatccg gcccggattg
     3661 gtagccacag tcggcccagt ctacagcgtg accgtgccac cccgcgctga gccggtcgcc
     3721 atcaggggcg tgctcatgga cggcaaaaca gagtggtcgg tctctctggc cgtcgagccc
     3781 aggtcggatg acgggatgaa ggtggatctg cggtccgtgt ggctgaccaa ggatctgctg
     3841 gccttcgaga cgaacccgga tcccggcaag tgcgagatgt acgtcggatg gccgatgccc
     3901 atgtgggctg ggagtggctt ctccacgaac cggcccgacc gcgaggtggg caagaacttc
     3961 gagcgcggct actgcgtcct cgttctcgtc gagcccgccg tgatgtagca ggcgacgaca
     4021 cgcctgcacc ctcttcgttc tccggcccga gactggtggc tgtcgccggt ttcgggccgg
     4081 agaaccgctg atcgtcaggc catcgtacgg gccgagcaag ccccgcccct acggcgagct
     4141 ggccggcgcc cacggcgtct cgaggtggta ccgtcgcgcg acggcatcga agatcgcggc
     4201 accgctggac tcgccccggt atgtgagtcc cgcccggggg ctcgagacgc gcccgccctc
     4261 gcggatgcga gacacgtcag tcgtcggcac gtggatggtc gccgtcgtgt tcgcgggcac
     4321 ctcgacgtcc atcaccagcc gatcgccgtc gagccgccac gcactgcgga tgcggccatg
     4381 tgggctgttg tagtccgcct tggcccaagc gaggccggcc acgggtcgcg gccatacggt
     4441 gaagtgctcg aagccgggat gatcagcatc ggggcggatc ccggccaggt actcgtagaa
     4501 ccactcgccc acaccgccca gggcgaagtg gttgcgcgag ttcatccccg ggccctcggt
     4561 gtcgctgttc cacagctccc agatggtcgt tgcgcccttc tcgaccatat agccccacga
     4621 ggggtaggtc cgctgggttg ccacccggta ggccaggtcg tgatagccca tgtcgctgag
     4681 gacgggcagc aggaaggccg tgccgaggaa gcccgtcgag agatgctcgt tgcggccgcg
     4741 cacgtcctgg gcgatgtggg cggccacctc agcgcgccga tcggccggcg tgatgccgaa
     4801 ggccagggga agcaggttcg cggtctgggt cccgccgggg tagttgccgt ccggcgccag
     4861 gtaggcctcg ttgaacgcgc gtgcgatgtc gtcggccagg gccaggtact gtttggcgtc
     4921 gaggtcacgg ccccgagcag cggccatctc ggcgagccgc cgggccgaaa ggtagtagta
     4981 ggccgtgccg atgggcttct tggcggttcc gacgggcgcg atccagtcgc catagccctc
     5041 gcgctcgtac agatggttgg gggcatggcg ccgcatgtac tcgacccatc cgcgcatcga
     5101 gtcgaagctc gtgtcgacca gacgggcgtc accatagaag cggaacacgt tccagggcac
     5161 gatggtgatc acatcggccc atgcggggga tccggccgat ggtgcgccca cgggagcgat
     5221 gtcggccacc tcgcctccgg ggccctggga gtcgcgcagg tccagcatcc acttcgcgaa
     5281 gaaggaggcc atatccatgt tccagcacgc ggtgggcgcg aatgcctggg cgtcgccggt
     5341 ccagccgagg cgctcatcgc gctgtgggca gtcggtcggc acgctgtgca tgttcgagcg
     5401 ctggccccac acgatgttgt gctggatctg gttcagtagc ggctcggagc actcgaagcg
     5461 tccgatgacc ggcgcatcgc tgtgcacggc ctcggcggtc agcgcgtcgg cggcaggctt
     5521 ccccgggaag ccggttacct cggcatatcg gaacccgcgg taggtgaacc gaggcgccca
     5581 cgtctcctcg ccatcaccgc gcaggatgta ggtgtccgtc gccttggccg accggtagtt
     5641 ctcggtgtag atgctgccat cgggattgag cacctcggcg aagcggatcc tcactgcggt
     5701 gcccgcggga cccttgaccc tcagccgctc gcgtcccgcc aggttctggc cgaagtcaaa
     5761 tacgaagacc cccggactgg gctcggtgat ggccttcggc gccaaggcgt gcgtacatcg
     5821 gatcgccggg ccccgctggg ccaccagccg gccgatgtcg tcggcgatga tcgcagtggg
     5881 ccgccactcg cggtcatcga acccgggctg atcccaaccg ggcatggcca ggcgcgcgtc
     5941 ataggtctcg ccatcgtaga ggtcatcgct gaggacaggc gattcgtgcg cgcgccacga
     6001 gtcatcggtg gcgacgagga cgtgctcgcc gttggccagc tccacatcga gttcgcagac
     6061 gaaccgcaac acgttacgca cgccgggcgg cggcgtgcgc cagctgatgc gtccggacca
     6121 ccagccattg cccaccacgg cgcccacggc gttccggccg gagcggagca gatcggtcac
     6181 gtcgaacgtc tggtactgga cccgcttgtc gtagagtgtc cagccgggcg cgagctcatc
     6241 ggcacccacc ttgcggccgt tgatccgcag ctcgtagagg cccaggccgg acacgtaggc
     6301 acgggctgac gtcacaggct gatcgaggcg gaactccttg cgaaagcact gggaccgcaa
     6361 gttccgcccg ccgccgccct ggcccggggt tccatagggt gccgcccccc actgggccac
     6421 gacgacgggt ggcacccagg cggagtcgtc atagccggcc gtggcccact cgccctcggc
     6481 atcggcagcc gtgcgccagc cctcggacgt ggtgatggct tgcgtctgcc catcctcgta
     6541 gcggatcagc aagccggcca cgaaggccgc ggggccatcg gcgttagtgg cctcagccgc
     6601 cacgacgttg gcgccggggc cgagcttccc ggtcacgtcc caggtcgata gcgtcttcca
     6661 gtcctggctc gagccgaggc gttc
//
LOCUS       DAOSPL010000039         6673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_11551_length_6673_cov_2.576458, whole genome shotgun sequence.
ACCESSION   DAOSPL010000039 DAOSPL010000000
VERSION     DAOSPL010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11551_length_6673_cov_2.576458"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PLQ54_01335"
     CDS             <1..318
                     /locus_tag="PLQ54_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91932.1"
                     /translation="PEIQPLPLVGTPPGKRIEIGVPIPDRRAWHARQWRSVLAHATAA
                     ERAMELANERQWKSEQAAARALWRAADAERARAERAADGLNEARWRRHGTAHRGRPTY
                     VLR"
     gene            complement(303..1478)
                     /locus_tag="PLQ54_01340"
     CDS             complement(303..1478)
                     /locus_tag="PLQ54_01340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOS91933.1"
                     /translation="MGWATLGSAGALTAAPFPKIDAASAIVEDRETGEVLFERAADET
                     REIASLTKIMSAIVAYEERPREPVAVVSAHAASLGEATAHLRAGERVRFDDLLALMMM
                     LSANDAAVAVAEHVAGSEQAFVARMNRWAERLGCYDTHFQNPHGLNADLHYSTARNVA
                     TMARHLLQIPEAARLVAAKELRIPRPGYRQDPMEVRNINSLLFWRPDVDGVKTGYTRR
                     AGGCLVSSARRGERGYIVVVLGSESRFHDSDALLTWAFESFDMRRVLEPGQPVHEVGI
                     AHGVRHSVKVTPGERLDLLLPVGQPAPAWDFRQQVHSAPIRAGAEVGTFTVWHRGVVY
                     EVPGVAAEAVDLTWWRRMVAWPDGALLLLSAGILAVSSGYLFHYLTRSRPMRDQRST"
     gene            complement(1531..2208)
                     /gene="scpB"
                     /locus_tag="PLQ54_01345"
     CDS             complement(1531..2208)
                     /gene="scpB"
                     /locus_tag="PLQ54_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOS91934.1"
                     /translation="MDLLTAKLALECLLFVADGPLDKKRLCSLIGIDGDLFEAALQGL
                     HEDLADRSLQVLEVAGGYRMMTRPAFASYAEALFEPSPERVSRSALEVLAVVAYRQPV
                     TRPEIDAIRGVNSSAALVSLLRKGLIREAGRKETPGRPILYATTDEFLKIAGLSGLED
                     LPHVDTPALDARVLGLPGAVAEHPGTPTAIADEGTELDRDEETGDCAPGEEPQANAET
                     DASAADA"
     gene            complement(2214..3020)
                     /locus_tag="PLQ54_01350"
     CDS             complement(2214..3020)
                     /locus_tag="PLQ54_01350"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOS91935.1"
                     /translation="MATRLAEPASRSEEASCWETYRVRLAAYEGPLDLLLYLVRRNEV
                     DVYEIPIAQITDQYLEYILLMGMLNIDVSGEFIVVAAQLLLIKSRRLLPLADEDDVAE
                     ADAPEEGEDPRRELERRLAEYRRYKESAEELRQRIELQSKRFTRAWDEAEAFATESVG
                     VESVSVFDIVTVFKEILSTARPEQPTVIQRQGLTVGRRMQELEALVARAVDGLSFHEA
                     IPPNPTRIEVVVTFLAILELVRRRRVAVQQHRLMGEIRIFALSRPRAAEV"
     gene            complement(3128..3745)
                     /locus_tag="PLQ54_01355"
     CDS             complement(3128..3745)
                     /locus_tag="PLQ54_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOS91936.1"
                     /translation="MTPELFASSIVAFVVAITIHEFAHAYAAYRAGDDTAKLAGRVSL
                     NPVDHLDPVGTILILLGGFGWGKPVPVNDLRLRRWRWHQIAVAAAGPGSNIATAAVLA
                     GVWHILSRLSPTGYPSEGAALLLLPTIGLSLALAVFNLIPVFPLDGHHIVGGLLPARM
                     SEAYGRGLGGPQWGMTVLFLLVVTRTTHVMIDPAVTFCLGVLGMG"
     gene            complement(3779..>5246)
                     /locus_tag="PLQ54_01360"
     CDS             complement(3779..>5246)
                     /locus_tag="PLQ54_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polya polymerase"
                     /protein_id="HOS91937.1"
                     /translation="THGVGQIPIMDGAQLVGIVSRADLRRALHGARMHQGAGGTGWCG
                     IERLDQLMEERLSAETLDVLRQIGRVGDACGVPVFLIGGAVRDLLLDIPNLDIDVVVE
                     GDGVELAERLAEAFSGKCSVHTRFGTATVRLDGGWKIDVASARTEHYVRPAALPSVEF
                     AGLKDDLYRRDFTMNAMAVQLNLDAFGRLIDYFGGRGDLEDGLVRVLHNLSFIDEPTR
                     IFRAIRFEQRYRFTMESQTEALLQYALESQFLDSLSSDRIRDELVLLLSEPDPVPAIH
                     RMAHLLVLEHIHRHLAFDEAVLEELHRTTRIIDWIADLDPPVAFDRWAVYLIPLLRDM
                     TPSEVRGLARRLHFTQRVIDALSQAREAADMVLAALSAEPDLKPSQVYDLLCDMPAES
                     IVHLIVRAGEGSIAERHLGAYLTTHRHVRLVVAGADLLSAGFAPGPGYGRVLRQVLTE
                     KLDGAIAGPEQERQRLFELAEASIPRRGRPPSRQKAGG"
     assembly_gap    5247..5256
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5257..6441)
                     /locus_tag="PLQ54_01365"
     CDS             complement(<5257..6441)
                     /locus_tag="PLQ54_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOS91938.1"
                     /translation="MDIILGHHGTDFDCLASIVAAGKLHPGAVCVLPRGVDRSVHQFL
                     GLYRDAFDLRQARYVDADAVRRIIVVDTRRVARLGPLGELIGKPGIEVILYDHHPALE
                     GDIVPTRAVTEPLGATVTLLLRELRARDAFITDTEATLYALGIYEDTGNLTYSSTTVD
                     DVLCVAYCLENGADLDVVNRFVRYDMSEEQRAVMNQFVLSAEHHDIHGVKIALAMAEV
                     ADYVGDVAALTNKLTLIESVDAVFTLASMKKAVYLVGRSRSDAVDAGTVLEGFGGGGH
                     HRAASAVVRGLTVEEVRAELLLRLQGAIAPPPVAAEIMSAPVRTINHDATVNEGYNLL
                     VRHGYGGVPVVEDGRMVGVISRKDIDVARMHGLGHAPVRGYMSRRVITAAPNTPGPEL
                     QAL"
     gene            complement(6455..>6673)
                     /locus_tag="PLQ54_01370"
     CDS             complement(6455..>6673)
                     /locus_tag="PLQ54_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOS91939.1"
                     /translation="VLISDTPSQPIDPGDVIAVQSTGAYNYAMASNYNRVPRPAVVFV
                     LDGAADIVVERETLDDMVAHDRIPDRLR"
BASE COUNT         1201 a         2243 c         2158 g         1061 t
ORIGIN      
        1 cctgagatcc agccgctgcc attggtcggc acaccgccgg gcaaacgcat cgagatcggc
       61 gttccgattc cagatcggcg agcatggcac gcacgtcagt ggaggagtgt tctcgctcac
      121 gcgacggccg ccgagcgggc gatggagctc gccaacgagc gccagtggaa gtccgagcag
      181 gctgcggcgc gggcgttgtg gagggcggct gacgccgaga gggctcgcgc cgaacgcgcc
      241 gctgatggcc tcaacgaggc ccggtggcgc cgccacggta cagcccaccg ggggcgaccc
      301 acctacgtgc tgcgctgatc ccgcatcggg cgagagcgcg tcaggtagtg aaagaggtag
      361 cccgacgaca cagcgaggat gccggccgag agcaggagca gtgcgccgtc gggccacgcg
      421 accatacggc gccaccatgt aaggtccacc gcctcggccg cgactccagg gacctcgtag
      481 acgacaccac ggtgccagac ggtgaacgtg ccgacctcgg ctcccgcgcg gatcggggcc
      541 gaatgcacct gttggcggaa gtcccaagcc ggggcaggct ggccgacggg caggagcagg
      601 tcgagtctct cacccggcgt caccttcacc gagtgacgga ccccgtgggc gatgcccacc
      661 tcgtgcaccg gctggcccgg ttccaaaacc cggcgcatat caaacgactc gaaggcccac
      721 gtgagcagcg catcggagtc gtggaatcgt gactcgctgc ccagaaccac cacgatgtac
      781 ccgcgttcgc cccggcgtgc cgaggaaacc aggcacccgc ccgcccgccg cgtgtagccg
      841 gtcttcaccc cgtcgacatc cggccgccag aacagcaggg agttgatgtt ccgcacttcc
      901 atcgggtcct gccggtagcc tggacggggt attcggagtt ccttggcggc gacaaggcgg
      961 gccgcctcgg ggatctgcaa caggtggcgg gccatcgtgg cgacattgcg tgccgtggag
     1021 tagtggaggt cggcgttgag cccgtggggg ttctggaagt gggtgtcata gcatcccaga
     1081 cgctcggccc agcggttcat gcgcgccacg aaggcctgtt cggagcccgc cacatgctcg
     1141 gccacggcaa cggccgcgtc gttggcggag agcatcatca tgagcgccag caggtcatcg
     1201 aaacgcacgc gctccccagc ccgaaggtga gcggtggctt cgcccagtga ggccgcatgg
     1261 gcactgacaa cggccaccgg ctcacgcggg cgttcctcat aggccacgat ggccgacatg
     1321 atcttggtca ggctggcaat ctcgcgtgtc tcgtcggccg cacgctcgaa cagaacctca
     1381 ccggtctcgc gatcctcgac gatggccgat gccgcatcga tcttgggaaa cggcgcagcg
     1441 gtcagggctc ccgccgagcc gagagtggcc caacccacga gcgcacccac caggaccggc
     1501 cgcccgagac cgctccgccg acgtccaggt ctacgcatcg gcggcggatg cgtcggtctc
     1561 cgcattcgcc tgtggctcct cccccggcgc gcagtcgccg gtttcctcgt cgcggtccag
     1621 ctcggtgccc tcgtccgcga tagcggtggg cgtacctggg tgctcggcga cagcacccgg
     1681 caggccaagg acccgagcat cgagtgctgg agtgtcgacg tgaggcaggt cctcaagtcc
     1741 gctgagcccg gcgatcttga ggaactcgtc cgtcgtggcg tagaggatag gtcttcccgg
     1801 cgtctccttg cgacctgcct cacggatcag ccccttgcgc aacaggctta cgagagcagc
     1861 cgaggagttg acgccgcgga tggcatcgat ctccggacgc gtcacgggct gccgataggc
     1921 caccaccgcg agaacctcca gggccgatct ggacacgcgc tcggggctcg gctcgaacag
     1981 ggcctcggca tagctggcga aggcagggcg agtcatcatg cggtacccgc cagccacctc
     2041 gaggacctgg aggctgcgat cggccaggtc ctcatgcagg ccctggagcg cggcctcgaa
     2101 cagatcgccg tcgatgccga tcaggctgca cagccgcttc ttgtcgagcg gcccatcggc
     2161 gacgaacagc aggcattcca gtgcgagttt ggcggtaagt agatccacca gacctacacc
     2221 tccgccgcac ggggccgtga cagagcgaag atccggatct cgcccatgag ccggtgctgc
     2281 tgaaccgcta cgcgtcgacg cctgaccagc tccagaattg ccaggaacgt caccacgact
     2341 tcgatgcgcg tggggttggg cggtatggcc tcgtggaaac tcagcccgtc gaccgcccgt
     2401 gccacgaggg cctcaagctc ctgcatgcgg cggcccacgg tgagaccctg gcgctggatc
     2461 actgtgggct gctcgggacg agcggtggag aggatctcct tgaacacggt cacgatgtcg
     2521 aagacggaga ccgattcaac acccacggat tccgtcgcga aggcctctgc ttcatcccaa
     2581 gcccgagtga accgcttcga ctgcagctcg atcctctggc ggagctcctc cgcggactcc
     2641 ttgtagcggc ggtactccgc cagccggcgc tcgagctcgc gccgtggatc ctcgccctct
     2701 tcgggcgcat cggcctccgc gacgtcgtcc tcatcggcca gcggcaggag tctccgggac
     2761 ttgatcagga gaagctgggc cgccacaaca atgaactcac ccgacacgtc tatgttgagc
     2821 atgcccatca gcaggatgta ctcgaggtac tggtccgtga tctgggcgat tgggatctcg
     2881 tagacatcga cctcgttccg tcgcaccaga tagagcaaca ggtccagcgg gccctcgtag
     2941 gcggccagcc gcactcggta ggtctcccag cacgaagcct cttcggaacg cgaagccggc
     3001 tcggctaacc tcgtcgccac cacataccct cccagctcga ttccggccgg cggcagccag
     3061 gccggtcgta cggcatgttc agcggtcggc cgtgcccctc gcggcccccg ggcacctcgt
     3121 tccgctgcta gcccatgccc aagacaccaa ggcagaatgt cacggcaggg tcaatcatca
     3181 catgcgtcgt gcgagtcacc accagaagga acagcaccgt catgccccac tgcggcccac
     3241 ccagtccccg gccgtacgcc tcgctcatcc gggccggcag caacccaccc acgatgtggt
     3301 ggccgtcgag tgggaagacc ggtatcagat tgaacacggc cagcgccaga ctgaggccga
     3361 tggtgggcag gagcagcagc gcggcgccct cgctcgggta gcccgtgggg ctgagccggc
     3421 tcaggatgtg ccacacgccc gccagaaccg ccgccgtggc gatattcgaa ccggggcctg
     3481 ccgctgccac ggcaatctgg tgccaacgcc accgccggag acgcaaatca ttgaccggca
     3541 cgggtttgcc ccagccaaat cctccaagaa gtatgagtat agtgcccacc ggatcgaggt
     3601 gatccaccgg gttgagcgag acgcggcctg ccagcttggc ggtatcgtcg ccagcccggt
     3661 aggcagcgta agcgtgcgca aactcgtgga tcgtaatcgc caccacaaac gccacgatgg
     3721 aactggcgaa caactccggc gtcatccgcc cctgcatgta ttcgacaagc actctgctct
     3781 agccgcccgc cttctgccgc gatggtggcc taccccgtct gggaatgctg gcctcggcaa
     3841 gctcaaacag ccgctgacgc tcctgctctg ggccggcgat agcgccgtcg agtttctctg
     3901 ttagcacttg cctgaggact cggccatagc cgggccccgg ggcaaacccc gcagagagca
     3961 gatcggcacc cgccacgaca agtctgacgt gtcgatgtgt ggtgagatat gcgcccagat
     4021 gccgctcggc gatgctgccc tctcccgccc ggactatcag gtgcacgatg gactcggccg
     4081 gcatgtcgca gagcaggtca tacacctggc ttggcttcag gtcgggctcg gcgctgaggg
     4141 ccgccaggac catgtctgcc gcctcacgcg cctgtgagag cgcatcgatc acgcgctggg
     4201 tgaagtggag cctccgagcc aggccccgca cctcgctcgg cgtcatgtcc cgcaggagcg
     4261 ggatgaggta caccgcccag cgatcgaatg ccacgggagg gtcgaggtcc gcaatccagt
     4321 ctattatacg ggtcgtccga tgcaactcct ccagcacggc ctcgtcgaag gcgaggtgcc
     4381 ggtgaatgtg ttccagcacc agcagatggg ccatgcggtg tatggcaggg acggggtcgg
     4441 gctccgaaag cagcagcacc agttcatcgc ggatgcgatc cgaggaaagg ctgtcgagga
     4501 attgactttc cagtgcatac tgcaatagag cctcggtctg ggactccatc gtgaagcggt
     4561 agcgctgctc gaagcggatg gcccggaaga tgcgggtggg ctcatcaatg aaactcaggt
     4621 tgtgcagcac gcgcaccaaa ccatcctcga ggtcgccgcg gccgccgaag tagtcgatga
     4681 ggcgaccgaa tgcgtcgagg ttcagctgaa ccgccatggc gttcatggta aagtcgcggc
     4741 ggtacaggtc gtctttgagc cctgcaaact cgaccgaggg caacgccgcg ggcctgacgt
     4801 agtgctcggt tcgcgccgaa gccacatcga tcttccagcc tccatcgagc cgcacggtgg
     4861 ccgttccaaa ccgcgtgtgg accgagcact tgcccgaaaa ggcctcggcc aacctctcgg
     4921 ccagttcgac accatcgccc tcgaccacca catcgatgtc gaggttcggg atgtccagca
     4981 agaggtctcg aacggccccg ccaatgagga acacgggcac gccgcacgca tcccccaccc
     5041 tgccgatctg gcggaggaca tccagagtct cggcactcag ccgctcctcc atgagctgat
     5101 caagtcgctc gatgccgcac caaccggtgc cccctgcgcc ctggtgcatg cgggccccgt
     5161 gcaacgctcg ccgaaggtcg gcgcggctca cgatgcccac aagctgggca ccgtccataa
     5221 tggggatctg gcccacaccg tgagtgnnnn nnnnnncagg gcctggagtt ccggtccggg
     5281 ggtgttgggg gccgcggtga tcacccgacg ggacatgtag cccctcaccg gcgcgtggcc
     5341 caacccgtgc atgcgggcca cgtcgatgtc cttgcgggag atgacgccaa ccattcggcc
     5401 gtcctcgacc acgggcaccc caccgtaacc gtggcgcacg agcaggttgt agccctcgtt
     5461 gacggtggca tcgtggttga tggtgcgcac cggcgccgac atgatctccg ccgccacggg
     5521 tggtggcgca atggcgccct gcagacggag cagcagttcg gcgcgcacct cttcgacggt
     5581 cagcccccgg acgacggccg aggccgccct gtgatggcca ccgcccccaa agccctcgag
     5641 caccgtgccg gcatcgacag cgtccgagcg gctgcggccg accaggtaga ccgctttctt
     5701 catcgacgcc agggtgaaca cggcgtcgac actctcgatg agtgtcagtt tgttggtgag
     5761 cgcggccaca tcaccgacgt agtcagcgac ctccgccatg gccagggcga tcttcactcc
     5821 atggatgtcg tgatgctcag ccgagaggac gaactggttc atcaccgccc gctgttcctc
     5881 ggacatgtcg taccggacga accggttgac cacatcgagg tcagcgccgt tctcaaggca
     5941 gtaggccaca cacagcacat cgtccacggt ggtcgatgaa taggtgaggt tccccgtgtc
     6001 ctcgtagatg cccaatgcgt acagggtggc ttcggtgtca gtgatgaagg catcgcgcgc
     6061 tctgagttcc ctcaggagca gcgtcaccgt agctcccaaa ggctctgtca cggcgcgtgt
     6121 aggcacaatg tcgccctcca gcgccgggtg gtggtcatac aagatgacct cgatgcctgg
     6181 cttgccgatc agctcaccca gagggccgag acgagccacc cggcgcgtgt cgaccacgat
     6241 gatccgccgg acggcgtccg catcgacgta ccgggcttgc cggaggtcga acgcgtcccg
     6301 gtagagccca aggaactgat gaacactccg atcgacacca cgtggcagga cgcacacggc
     6361 gcccggatgg agcttgccgg ctgccacgat ggaggcgagg cagtcgaagt cggtcccgtg
     6421 atgtccgaga atgatgtcca tagccggtct cgggctagcg caatcggtcc gggatgcggt
     6481 cgtgggcgac catgtcgtcg agcgtctcgc gttccacgac gatgtcggcg gccccgtcca
     6541 ggacgaagac cacggccggt cgcggcaccc gattgtagtt ggatgccatg gcgtagttat
     6601 aggcgccggt ggattgcacc gcgatcacgt cccccgggtc gatgggctgg gatggtgtgt
     6661 cactaatgag cac
//
LOCUS       DAOSPL010000040         6592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_11759_length_6592_cov_2.831268, whole genome shotgun sequence.
ACCESSION   DAOSPL010000040 DAOSPL010000000
VERSION     DAOSPL010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6592
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11759_length_6592_cov_2.831268"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PLQ54_01375"
     CDS             <1..403
                     /locus_tag="PLQ54_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOS91940.1"
                     /translation="DRLVQRDPYVLAEVHYVRDRDAEFVERAGDDLAPQLVEAFCAFI
                     RLAQRVLGPESPTVRLPQNPVELSYAVCAALDLPPIEKQRMLELTSWRERARLALGLL
                     ERESRELSMMLASRPRPCRVDACCHDAGSLN"
     gene            527..1867
                     /locus_tag="PLQ54_01380"
     CDS             527..1867
                     /locus_tag="PLQ54_01380"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOS91941.1"
                     /translation="MWVLSLTLLEALGLVVPCAGSQAMGPGLGSLSSLPRLGGPPPLR
                     ASSADASGKNADYVPIAPGETKTIFDEPGPGCIRHLWVTLKSRPTKLFNIPDQHLRWI
                     VIRMFWDGEQHPSVEAPIGDFFGLGHAEYYSYESAVMSAAVRRGLNCYLPMPFYKRAR
                     IEVQNQSDQLGLKEGGVEVQKFYYNIDYERYTEPLPPDYGLLHAQWRRQNPTPAVKGT
                     DEKGRFSDNYVILDAKGKGRYVGCNLSVHSLNPNEWWGEGDEMMAIDSAEPNVLGTGT
                     EDYFCCAWGFPEAFSSPYYGVPYLDSEEQTEKTTAYRWHIEDPVMFAQSIIVSIEHGA
                     GNHRADDFSSTAYWYQTEPHVPFATLPAVKDRLPLARKTPMPAPKTGPSPKVAAQPAG
                     PQRAELDEADMARVAAAARAQVLWCVWVPVGLAVGAVALNRWARRRGRSSSGGQ"
     gene            1886..3214
                     /locus_tag="PLQ54_01385"
     CDS             1886..3214
                     /locus_tag="PLQ54_01385"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOS91942.1"
                     /translation="MDEPERDALGGMAAGRGAWAGAREGLREQRRFVRIEGRFAAAYE
                     VVATDTGQRSPARRGIVDNLSMGGARLVTEHPLQPGLLVTMRFPITTPEGPDLVLVDG
                     RVVRCYPDPRPGECACGIEFTDISLEEAKRLAYLLENMARQPAPMEKSVAALFDSLRS
                     FAHRAATLGDPQALLDELVQAAAEVTAAPGGCVLLLDRTGEYLHFAAARGASLDALSR
                     FRVAVGQGIAGLVALHAKPMNIARPYDDPRWFAEVAESVGVETDSLLAVPIMFRERVI
                     GVLEVLNKPDADRFSPTDILALDLLATHAAVALQCLRMELAPITALHHVDRGTEQAER
                     RTSDSQTDDAGGRPKALAVCVHGAGTPLFTTEARSLLEQLDPTGVLAYEVQNLLTFAV
                     DPSRRESMDGAEREIDAPDGRCLFIRFAALEGDGEEPAGLVAYLGAAARP"
     gene            complement(3261..3995)
                     /locus_tag="PLQ54_01390"
     CDS             complement(3261..3995)
                     /locus_tag="PLQ54_01390"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOS91943.1"
                     /translation="MSHAAAPEGTGPCAPSIPLIGDNAPAFRAVTTQGEISFPEDYKG
                     KWVILFSHPADFTPVCTTEFMTFASMQEQFRALNCELIGLSIDSHYSHIAWLRTIREK
                     ISYKGMSHVEVTFPVIADLKMDVAKAYGMVQPGAADTQAVRAVFFIDPQSKVRALIYY
                     PLANGRNFDELLRLLIAMQTSDAHQCATPADWRPGDDVIIPPPGSCGSAKERVDGAGS
                     GGYECLDWFLCLKKLPKSELKLPPGV"
     gene            complement(4168..>6592)
                     /locus_tag="PLQ54_01395"
     CDS             complement(4168..>6592)
                     /locus_tag="PLQ54_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91944.1"
                     /translation="PKRFELVLQRGRDHVAVLVDGQRILSADPAMEPVGRIGSGVSGA
                     VSITDLLLQPTGDLALSDDFTRDTTDMATWEVLEGKWANTVQASEGFLSDFTANPFAF
                     RADAAPRAMARTGEWFWIDYSIGVSVRPVSTQAVGLAAHVHDPANLVLLRWRAGGNGD
                     PDCRQLSVVRNGKEQVLASAPGGYVPGQWYRLALRVGGRRVTAFINGFPCLSAETPLL
                     SQGPGAICAWQGQAFFDDVTVAHENETPDPPVLVPEQFAKDELMQQLGVTNPRTMWLG
                     PTNGAFWYWGLFPEQARIELPLAPLQGQQATIVMRGDGANPSAAYMVGLQWANDALSI
                     DLKRAGESIDQRSVPCPSDGVVEAEVDSGLIRVLVDGRSVYERTDPTPLAGAWVGAMV
                     PDVNVLAEAIVTSRHQLDDTFTSAPTHWLAAKGTWGVDVRWPCDRRWAFFGGKDDENP
                     TVWSKQVLKGDVAVETFAAVRMDIPHCPGYSHPSDLCITLCGNGRDVGSGYSFIYAGF
                     NNTKSCILRQGQVVAEAPGQVFQGAVNTNLEFQRAWFRLRAEKIGNRLRLYLRDTPIC
                     EYHDPDPLPDGQAALWSYRNGPIYARVRTWASGAEPAPLPRVYSALPATPAPYRWPEG
                     LLFDFEQNTGEFHATAQSGTRVYSDPERAATGKRSLKVVNTVSGGPFAAYAIVEPFCI
                     ADWPELSFDYAIPPNVRVNLYLYARQAWHAIELTGGAPEDSSRVRPIGRIPDVVADGQ
                     FHSARLSLKEAFVGLYPDSRIRVAAMAFASPETVQDYVRCGIGGNAKDSAYWIDNFRL
                     GVPGAPVGR"
BASE COUNT         1099 a         2176 c         2242 g         1075 t
ORIGIN      
        1 cgatcggctg gtgcagcggg acccgtacgt gctggcggaa gtccactacg tccgtgaccg
       61 cgacgcggag ttcgtcgaac gggccggaga cgacctggcg ccgcagctcg tcgaggcctt
      121 ctgtgccttc atccggctgg cccagcgggt gctcggtccc gagagcccta cggtgcgcct
      181 gccgcagaac ccggtcgagc tctcctacgc ggtgtgtgcg gcactggacc tgccaccgat
      241 cgagaagcag cgaatgctcg agctcacctc gtggcgcgag cgggctcgac tggcgctggg
      301 actgctcgag cgcgaaagcc gtgagctgtc catgatgctt gcgtcacgac cgcggccatg
      361 ccgtgtcgac gcgtgctgcc acgacgccgg cagcctgaac tagccgcgcc cccttccgct
      421 ataatgctcc gcagtccgtc cggagcggcc gcccggccgc tcgtcgcgac gacgcgccgg
      481 ctccggcgcc aacacgtccg gcaaaggcgg gttgcggatg cgccgcttgt gggttctgtc
      541 cctgacactt ctggaggccc ttggcctggt tgttccctgc gccggttcgc aggccatggg
      601 gcccggcctc ggctcactgt cctcgctgcc gcgcctgggc gggccgcctc cgttgcgggc
      661 ctccagcgcc gatgcctcgg ggaagaatgc cgactacgtg ccgattgctc cgggggagac
      721 caagacgatc ttcgacgagc ccgggcccgg atgcattcgc cacctctggg tgacgctcaa
      781 gagccgcccc acgaagctgt tcaacatccc cgatcagcac ctgcgctgga tcgtgatccg
      841 catgttctgg gacggcgaac agcatccgag tgtcgaggct ccgatcggcg acttctttgg
      901 cctgggtcat gccgagtact actcgtacga gtcggccgtc atgtcggcgg ccgtccgccg
      961 gggcttgaac tgctatttgc ccatgccgtt ctacaaacgg gcccggatcg aggtccagaa
     1021 ccagagcgac cagctgggcc tcaaagaggg cggggtcgag gttcagaagt tctactacaa
     1081 catcgactac gaacggtaca ccgagccact ccctcccgac tacggccttc tccacgctca
     1141 gtggcggaga cagaacccga cgccggcggt caagggaacg gacgagaaag ggcggttctc
     1201 ggacaactac gtgatactcg acgccaaagg caaaggtcgg tacgtgggat gcaacctgag
     1261 cgtgcactcc ctcaacccca acgaatggtg gggtgagggg gatgagatga tggccatcga
     1321 cagcgccgaa cccaatgtgc tgggcacggg cacggaggac tacttctgct gcgcctgggg
     1381 tttccccgag gcgttctcgt caccctacta cggtgtgccc tacctggatt ccgaggagca
     1441 gacggagaag accaccgcct accgatggca tatcgaggat ccggtgatgt tcgcccagag
     1501 catcatcgtc tcgatcgagc atggtgcggg caaccacagg gccgatgact tctcgagcac
     1561 ggcctactgg tatcagacgg agccccacgt gcccttcgcg acactgccgg cggtgaagga
     1621 ccggctcccg ctggcgcgca agacgccgat gcccgcgccc aagacaggac cctcgcccaa
     1681 ggttgcggct cagcctgccg gaccgcagcg cgccgagctg gatgaagcgg acatggcccg
     1741 agtggccgcc gcggctcgcg cccaggtcct gtggtgcgta tgggtgcccg tgggtctcgc
     1801 cgtcggcgcc gtggccctga accgctgggc gcggcggcgt ggccgaagct cgagcggtgg
     1861 gcagtagacc aaggaggcct ggccgatgga tgagcccgag cgggacgcgc tcggtggcat
     1921 ggccgccggg cgcggggcgt gggccggtgc gcgcgagggg ttgcgggaac agcgccggtt
     1981 cgtccgcatc gaaggccgat tcgcggctgc ctacgaggtt gttgccacgg acacgggcca
     2041 acgaagcccg gcgcggcgcg gcattgtcga taacctcagt atgggcggcg cccggctggt
     2101 caccgagcac ccgctccaac cgggcctgct tgtgacaatg agattcccca tcaccacgcc
     2161 ggaaggtcct gacttggtcc tcgtcgatgg ccgcgtggtg cgctgctacc ccgaccctcg
     2221 tcccggcgag tgtgcctgcg gcatcgagtt caccgacatc agcctcgagg aggcgaagcg
     2281 gctggcctat ctcctcgaga acatggcccg gcagcccgcc ccgatggaga agtcggtggc
     2341 tgccctgttc gactccctgc ggtccttcgc tcatcgcgcc gcgacactgg gcgacccgca
     2401 ggcgctgctg gacgagttgg tccaggctgc cgcggaggtg acggcggcgc caggcggctg
     2461 tgtgctgctc ctggaccgca ccggcgagta cctccacttc gcggccgcgc ggggcgccag
     2521 tctcgacgcc ctgtcccgat tccgcgtggc cgtgggccaa gggatcgcgg ggcttgtggc
     2581 ccttcacgcc aaaccgatga acatcgccag gccctacgac gatccgcgct ggttcgccga
     2641 ggtggccgag tcggtaggtg tcgagacgga cagtttgctg gcagtcccta tcatgttccg
     2701 cgagcgggtc atcggtgtgc tggaggtgct taacaagcca gacgccgacc ggttctcgcc
     2761 caccgacatc ctggccctcg acctcttggc cacgcacgcg gcggtcgcac tccagtgtct
     2821 gcgaatggag ctcgcgccga tcaccgccct gcaccatgtg gaccggggga ccgagcaggc
     2881 ggaacggcgc acgagtgact cgcagaccga cgatgccggt ggtcgcccga aggcgctggc
     2941 agtgtgcgtg cacggcgccg gaacgccact attcacaacc gaggcccgga gcctactgga
     3001 gcagctcgac cccactggcg tcctggccta tgaggtgcag aacttgctga cgttcgcggt
     3061 ggatccctct cggcgggagt cgatggacgg tgccgagagg gagatcgatg cgccggacgg
     3121 gcgctgcctc tttatccgtt tcgcggcgct cgagggcgac ggcgaggagc cggcaggtct
     3181 ggtcgcctac ctgggcgcgg cggcaagacc ataggaagac cgggggcggc gggcgtgccg
     3241 cccccggcca ggtgcccgat tcacacaccg ggcgggagct tcagctccga cttgggcagc
     3301 ttcttgaggc acaggaacca gtcgagacac tcgtagccgc cactcccagc gccgtcgacc
     3361 cgctccttgg cggacccaca tgaccccggc ggcgggatga tcacgtcatc gccgggccgc
     3421 cagtcggccg gcgtggcaca ctggtgcgcg tccgaggtct gcatcgcgat cagcaaccgg
     3481 agaagctcgt cgaagttccg tccattggcc agcggatagt agatcagtgc ccgcaccttg
     3541 gactgggggt cgatgaagaa cacggcccgg acggcctgcg tatccgcggc tcccggctgc
     3601 accatgccgt aggccttggc cacgtccatc ttcaggtcgg cgatcaccgg gaacgtgacc
     3661 tcgacatggc tcatgccctt gtagctgatc ttctcgcgga tggttcgcaa ccaggcgatg
     3721 tggctgtagt gggagtcgat ggagaggccg atcagctcgc agttcagggc ccgaaactgc
     3781 tcctgcatgc tcgcgaatgt catgaactcg gtggtgcata cgggcgtgaa gtctgccggg
     3841 tggctgaaca ggatcaccca cttgcccttg tagtcctccg ggaagctgat ctctccctga
     3901 gtcgtgaccg ccctgaacgc cggcgcgttg tcgccgatca gggggatgga tggcgcgcag
     3961 ggaccggtgc cctcgggcgc ggccgcatgg ctcattggga ccactcctcg tctgctgttg
     4021 ctgatgcggt gtcgggggtt ttggaagctc accgccgaca acgagtgcaa caaacaacga
     4081 gccgaatgtg ttccgcacat atagcagtgc tgggggtctg gggacgcgaa tcgagtgtgt
     4141 gcggccggcc agtccgacgc cgaggcctca gcgcccgacg ggcgcgccgg ggacacccag
     4201 ccggaagttg tcgatccagt aggccgagtc cttggcattg ccgccgatgc cgcagcgaac
     4261 gtagtcctgc acggtctccg gactggcgaa agccatcgcg gccacacgga tgcgcgagtc
     4321 cgggtagagc cccacgaagg cttccttgag gctcaacctg gccgagtgga actgaccatc
     4381 ggccacaacg tccggtatcc ggccgatggg gcgcactcga ctgctgtcct ccggggcgcc
     4441 gcccgtgagc tcgatcgcgt gccacgcctg gcgggcatag aggtaaagat tgaccctcac
     4501 attgggcgga atggcatagt caaacgacaa ctcaggccag tcggcgatgc agaagggctc
     4561 cacaatggcg tacgcggcaa agggccctcc cgacacggtg ttgaccacct tcagactccg
     4621 cttccccgtg gccgcccgct ccgggtcaga gtagacccga gtgcccgact gggccgttgc
     4681 gtggaactcg ccggtgttct gctcgaagtc aaagagcagg ccctcgggcc accggtacgg
     4741 cgcgggcgtc gcgggcaggg cggagtagac gcgcggcagc ggggcgggct ccgctccgct
     4801 tgcccaggtc cgcacacgag cgtagatggg cccgttgcgg tagctccaca gagcagcttg
     4861 gccatcgggt agcgggtcgg ggtcatggta ctcgcagatg ggcgtgtctc ggagatacag
     4921 ccgaaggcgg ttaccgatct tctcagcacg caggcggaac caggcgcgct ggaactcgag
     4981 gttcgtgttc accgcgccct gaaagacctg tcccggagct tcggccacaa cctggccctg
     5041 gcggagaatg cagcttttcg tgttgttgaa ccctgcgtag atgaagctgt agccactgcc
     5101 cacgtcgcgg ccattcccac acagcgtgat acaaaggtcg ctcggatgcg agtagcctgg
     5161 acagtgtggg atgtccatgc gcacggcggc gaaagtctcg acggccacat cgcccttgag
     5221 cacttgcttg ctccataccg tcggattctc gtcatccttg ccaccaaaga aagcccatcg
     5281 gcggtcgcat ggccagcgta cgtcgactcc ccaagtgcct ttcgccgcca accagtgggt
     5341 gggagcactc gtgaacgtat cgtcgagctg gtgccgcgag gtgacaatgg cctcggccag
     5401 cacgttgacg tcgggcacca tggctccgac ccacgcgcct gccagcggcg tggggtcggt
     5461 gcgctcatag acgcttcggc cgtcgacaag cacccggatc aggcccgagt ctacctccgc
     5521 ctcgaccacg ccatccgatg gacagggcac gctgcgctgg tcgatggact cgccggcgcg
     5581 cttcaggtcg atgctcaggg cgtcgttcgc ccactgtagg cccaccatgt aggcggcaga
     5641 cggattcgcg ccatcgcctc gcatcacgat ggtcgcctgc tggccttgga ggggtgccag
     5701 cggaagctcg atgcgcgcct gctcggggaa cagcccccag taccagaagg cgccgttggt
     5761 gggcccgagc cacatggtcc gcgggttggt gacacccagc tgctgcatga gttcgtcctt
     5821 ggcaaactgc tcgggaacca acaccggggg atcgggagtc tcgttctcgt gcgccacggt
     5881 gacgtcatca aagaaagcct ggccctgcca tgcgcatatg gcgccgggcc cctgggagag
     5941 cagaggcgtc tcggccgaga gacaggggaa gccattgatg aaggccgtga cccgccgccc
     6001 cccgacgcgc agcgcgagcc ggtaccactg gcctgggacg tagccacccg gagcggacgc
     6061 caggacctgt tccttgccgt tgcgcaccac ggagagctga cggcaatcgg ggtcgccatt
     6121 gccgccggcc cgccaccgga gcagcaccag gttggcgggg tcatgcacgt gagccgcgag
     6181 tccgaccgcc tgcgtggaca cgggccgcac cgagacacca atgctgtagt caatccagaa
     6241 ccactcgccc gtccgggcca tggcgcgcgg ggcagcgtcg gctcggaagg cgaaagggtt
     6301 ggcggtgaag tcgctcagga agccctcgga ggcctgcacg gtgttggccc acttgccctc
     6361 gaggacctcc caggtggcca tgtctgtggt atcgcgcgtg aaatcgtcgg agagagccag
     6421 gtctccggtc ggctgaagta gcaggtcggt gatggatacg gcaccagaca cacccgagcc
     6481 gatacgaccc accggctcca tggcggggtc ggccgagagg atgcgctggc cgtctaccag
     6541 cacggccaca tggtcgcggc cgcgctgcag cacgagctcg aaccgcttgg gc
//
LOCUS       DAOSPL010000041         6578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_11793_length_6578_cov_2.893301, whole genome shotgun sequence.
ACCESSION   DAOSPL010000041 DAOSPL010000000
VERSION     DAOSPL010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11793_length_6578_cov_2.893301"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PLQ54_01400"
     CDS             <1..421
                     /locus_tag="PLQ54_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91945.1"
                     /translation="QLRVREGEWVETVKDFCGWEQAARKSPSWNVTVKSGPEDRPLEC
                     MSLRSVFVDPWTGEIIHTAQWEGAGPAGAEALPSAIGGQLRPGRPGSPAALPTAQPLT
                     AGQAERSAHGRWLLAAGAGILVLALAAAMVCARRRRA"
     gene            complement(496..2601)
                     /locus_tag="PLQ54_01405"
     CDS             complement(496..2601)
                     /locus_tag="PLQ54_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91946.1"
                     /translation="MMTTAELRVIARHAGLWLLIACAAQSPTAAQVQLVAGGGFEGQY
                     REGSYLGVTIDLTNGSKSTRGELSAGFGSGPASKVRYTQHVDLPPGAHKRTTLYVRPS
                     SFENEVHVRFTPERGRPVIARAQLAYSDASSVVAYVIHDQGSGYHFIRSIDTSKWAAP
                     AGSAVAPGVGGPGGSSTTAVSVAYSDTTHMPDSWKGYDSADLVILDEHSPSALTADQA
                     QALKEWVAMGGHLLVVGGEDWQRLEQGPFADMLPVDVRGELPMAGGLPELAATYGGSM
                     PPGTPVITLCQARKGAETLLSHEGRPLIVRGAHGVGHVYFFAFPVDRQPIKDWSGLQS
                     LISQVIQDIRSRSPRLAAGISEPPVCDGLCENPSMAVPSFRVVGLFLLAYLICLVPAN
                     YFVLKRLGKRELAWLTTPAIVIVFTLIAYVMGLIMRGGDVVVYSASVVEVRAGSPFGV
                     SSGYFGIFSPRKTRYRIAFSQPHVAIDVPNRATSGFGGFEEVEVPAMTLASDGGEDSV
                     RSMLINMWDQQLLEARAATALEGAVTADLRVADGELVGEVHNGTSMTMAQTAIRYRSM
                     TKGLGDIAPSASAPVRASAPASGAMPAPATHPGAGPMGVMPYMGSPSGTPEGRDAFNQ
                     LMPIMGPWPTSAADDPDSAIRRGLLGGLLERGRPVFADSEALVFAWLDGQPLRIDLGG
                     ARYRSIGRTLLVVHVPVSE"
     gene            complement(2760..3512)
                     /locus_tag="PLQ54_01410"
     CDS             complement(2760..3512)
                     /locus_tag="PLQ54_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOS91947.1"
                     /translation="MAGHSKWHNIRLRKGAQDAKRGKQFTKVAREIIVAAKSGGGDPD
                     ANPRLRMAIREARSINMPNDNIERAIKKGCGELGPGETYEEAMFEGYGPHGVALLIAT
                     LTDNRQRTVPEIRSILGKRGGNLGEGGCVSWMFERKGVINVEKTKATEEDLFEAALDA
                     GADDVIDEADTWRVTCPPEAYEDVLKALETAGIPVASSEVTMLPSTSVKLGEKEAPSI
                     LRLLDDLEDIDDVQKVYANFDIPDEVLEAISG"
     gene            3774..5180
                     /locus_tag="PLQ54_01415"
     CDS             3774..5180
                     /locus_tag="PLQ54_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOS91948.1"
                     /translation="MRRVPRAVYGFLMPNFLGFLAFTVFPVVLSLWMAFTNWTLKPAL
                     GFEVLGLRNFTDLLGVRPLDRPVQGLWFAYMVGATAFCLGIVGTLWSSVAGGRGTRLG
                     GVVLTALGIGSVTVSLGFHGGQGKLISGLICLAFGLVAMRRDDMDWGIGCGALSAMLL
                     AAGAVAMMALRGPMWTVFELKDPRFWQYFYNTVYLMLGIPFSIAGSLALALLLNDELR
                     LGESVGRVIGAGLCLVGGAVTLAVIWETSPDLGLLGAVLWGVAALGFAANVVSFRTLF
                     YLPSFTAGVALMILWKALYNPDTGPINVGLKAVFDTFGVAAEPPKWLASVEWAKPALI
                     MMGVWTGIGGTNMLLYLAGLSNVPRDLLDAAEVDGAGTWARFRHVTWPQLAPTTFFIS
                     IMSIIGGLQGGFDQARVMTGGGPAGSTTTLSYYVYNKAFQDLDLGYAAAISWVLFAMI
                     FVATAINWRFGRELEVGP"
     gene            5177..6028
                     /locus_tag="PLQ54_01420"
     CDS             5177..6028
                     /locus_tag="PLQ54_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOS91949.1"
                     /translation="MTRRKLSNAARFVILCIIGFSICIPFVWMVSASFKPRAEVEEVN
                     IVPHQPTTRNYPIVMDLEPDPVTGKKLNMRFGRWYFNSVFIAVGVTFLQVLTSAMAAY
                     AFSRIRWAGRDTVFLLYLATMMIPGVVTMIPNFQIMVSLGFLNTYRGLVLPAAFSAFG
                     TFLLRQFMLSIPRSLDEAAEIDGARHWQIFWDVIMPLARPGLIALAIITFLGAYQSFF
                     WPLIMLNDAQFYPMPVGMLALDSSYGRQTELIMAATVMNVVPLIIIFVLFQKFLVKGL
                     QLGGVKG"
     gene            6033..>6578
                     /locus_tag="PLQ54_01425"
     CDS             6033..>6578
                     /locus_tag="PLQ54_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOS91950.1"
                     /translation="MKWVFAGVLLLFAGMGLVAWATLVPAETPGVVRIRWATDANPAR
                     DVQTRLFSEMYPGVEVVVDPGLGGDQTKLIVQCATGTGPDVVDVYSKEQMMALVDAGI
                     LLDLTPYAETMGFGVDKTYPAMEGGLVVDGRQYRFPCNVWANCVVYNKRIFDDHGVPY
                     PRDGWTWDEFIRVGKQLKETPS"
BASE COUNT         1047 a         2131 c         2182 g         1218 t
ORIGIN      
        1 ccagctacgc gtccgggagg gcgaatgggt agagacggtc aaggacttct gtggatggga
       61 acaggcggcc cgcaagagcc cctcgtggaa cgtgacggtc aagtcggggc cagaggacag
      121 accacttgag tgcatgtccc tccgcagcgt gttcgtcgac ccctggacgg gcgagatcat
      181 ccacacggct cagtgggagg gcgcgggccc tgctggggca gaggcactcc cttccgccat
      241 cggtgggcaa ctcagaccag gcagaccagg cagcccagcc gccctcccga cggcgcagcc
      301 gctgacggct ggacaggccg aacgctcggc gcatggccgg tggctgcttg cggccggcgc
      361 cggcattctt gtgctcgcgc tcgcagcagc catggtgtgc gcgcggcgaa ggcgggcgtg
      421 aggacgaaca ggcggaacgg cacgtgggag tgccgtccag gcggcgcgcc cgcagcgacc
      481 gctcggaggc gcgatctact cactgacggg cacgtggacg accagaagcg ttcggccgat
      541 cgagcgatag cgtgcgccgc ccaggtcgat ccgtagcggc tgaccgtcta gccaggcgaa
      601 caccagggcc tcgctgtcag cgaagaccgg tcggcctctc tcgaggagcc cgccgaggag
      661 tcctcggcgg atcgccgagt ccgggtcgtc ggcggcgctg gtcggccaag gtcccatgat
      721 gggcatgagc tggttgaagg catctcgtcc ctcgggggtg ccggacggcg agcccatata
      781 tggcatcaca cccatggggc ccgcccccgg atgcgtcgcc ggagccggca ttgccccgga
      841 cgccggtgcc gacgcccgta cgggtgccga ggcgcttggc gcgatgtcgc ccaggccctt
      901 cgtcatcgac cggtaccgga tggcggtctg ggccattgtc atcgaggttc cattgtgaac
      961 ctcgccaacg agctcgccat ccgccacacg gaggtcagcc gtgaccgcgc cctcgagcgc
     1021 cgtggcggct cgcgcttcca gtagttgctg atcccacatg ttgatgagca tagatctgac
     1081 cgagtcctca ccgccatcgg aggccagggt catcgccggg acctcgacct cctcgaaccc
     1141 gccgaaaccc gacgtggccc ggttgggcac gtcgatagcc acatggggct ggctgaatgc
     1201 gattcggtag cgggtcttcc ggggagagaa gataccgaaa taaccactcg aaactccgaa
     1261 tgggctgccg gctcgcacct cgaccacgga ggcgctgtag accaccacgt cgccgccccg
     1321 catgatgagg cccatcacat acgcgatcaa cgtgaacacg atgacgatgg ccggagtggt
     1381 gagccaggcc aactccctct tcccgagccg cttcagcacg aagtagttgg ccggaacgag
     1441 gcagatgagg tacgccagga ggaaaaggcc gacgacccga aacgagggaa cggccatcga
     1501 tgggttctcg cagaggccgt cgcacacggg aggctcactg ataccggcgg ccagccgcgg
     1561 cgaccgcgag cggatgtcct gaatgacttg ggatatcagc gactggaggc ccgaccagtc
     1621 cttgatgggc tggcggtcca cggggaacgc gaagaagtac acatgcccga caccgtgggc
     1681 cccacggacg atgagcgggc ggccctcgtg gctgaggagc gtttccgcgc ctttgcgggc
     1741 ctgacacagc gtgatcacgg gcgttcccgg gggcatcgag ccgccgtatg tggcggccaa
     1801 ctccggcagc ccgcctgcca tcggcagctc gccgcggacg tccacgggca gcatatccgc
     1861 gaacggcccc tgctcgaggc gctgccagtc ctcaccaccg accaccagca gatgcccgcc
     1921 catcgccacc cactccttga gggcctgggc ttggtcggcg gtgagggcgg acggtgagtg
     1981 ctcgtcgaga atcaccaggt cggcggagtc ataccccttc cagctgtccg gcatgtgcgt
     2041 cgtgtcggag tacgccaccg agaccgccgt cgtcgacgag cccccggggc cgcccacgcc
     2101 cggggcgacc gcgctgccgg ccggcgcggc ccacttggag gtgtcgatcg accggatgaa
     2161 gtggtacccg ctgccctggt cgtggatcac gtaggccacg acggaggagg cgtccgagta
     2221 ggcgagttgc gcacgcgcga tgacgggtcg tccgcgctcg ggcgtaaacc gtacatggac
     2281 ctcgttctcg aagctactgg gacgcacgta gagcgtggtg cgtttgtggg ctccgggagg
     2341 taggtccaca tgctgggtgt agcgcacctt cgaggccggt ccggagccga aacccgcgga
     2401 taactcgccg cgcgtgctct tgctgccgtt ggtcaggtcg atggtgaccc caaggtagga
     2461 gccctctcgg tactgaccct caaacccgcc ccccgcgacc aactgaacct gcgcggccgt
     2521 gggactctgc gcggcgcagg cgatgagaag ccacagaccg gcgtgccggg caatgacccg
     2581 aagctcagcg gtggtcatca ctacccccgt ctatcctgtc agcgggcgcg actgcggatg
     2641 ggaatcagga ccgccgcgca cctcaggcac agatggccat ccggacgtcc gggctgggtg
     2701 aaagtagtgg tgggggttcg ccggaccgtt cgcggcccct gccgacagcg gtctgagggc
     2761 taacccgaga tggcctcgag tacctcgtcg gggatatcga agttcgcgta gaccttctgc
     2821 acgtcgtcga tgtcctcgag gtcgtcgagg agccgcaaaa tgctcggcgc ctccttctct
     2881 ccgagcttca ccgaggtgga gggcagcatg gtgacctcgg acgaggcgac ggggataccc
     2941 gcggtctcca gtgccttcag cacgtcctca taggcttccg gagggcacgt gacccgccac
     3001 gtgtcggcct catcgatgac gtcgtccgcg cccgcgtcga gggcggcctc gaacaagtcc
     3061 tcctcggtcg ccttggtctt ctcgacgttg atgacgccct tgcgctcgaa catccacgac
     3121 acacagccgc cctcacccag gttcccgcct cgtttcccga gtatgctccg gatctcgggc
     3181 acggtgcgct ggcggttgtc agtgagggtc gcaatgagaa gggccacgcc gtgcgggccg
     3241 tacccctcga acatggcctc ctcataggtc tcgcccgggc cgagttcacc acagcccttc
     3301 ttgatggccc gctcgatgtt atcgttgggc atgttgatgg atcgggcctc gcggatggcc
     3361 atgcggaggc ggggatttgc gtccgggtcg cctcccccgg acttggcggc cacgatgatc
     3421 tcacgggcca ccttggtgaa ctgcttgccc cgtttggcgt cctgggcgcc cttgcggagg
     3481 cggatgttat gccatttcga gtgaccagcc atcggcggtt gccttggagc ccctttcccc
     3541 aagcaccccc aaacgtgcat tcggggcgtc tcttgcgatt atgcggagcc atggagggcg
     3601 tgtcaaggag cccgcgcccg gcggctacag accaggtgac gcgccggggc ggattccggt
     3661 ggccgcgcac gcgtctctgc tctgctggcc cgcctctcgg ccgttgacag cccccggcgg
     3721 agcgtgtacc gtccgagttc gaacgccttc tgaggatggc gaccgctacc ccgatgagac
     3781 gcgtgccaag agccgtctat gggtttttga tgcccaactt tctgggcttc ctcgcattca
     3841 ccgtcttccc cgtggttctc tccctatgga tggcgttcac gaactggacc ctcaagcccg
     3901 cactgggctt cgaggttctt ggccttcgca acttcaccga cctgctgggc gtacggccac
     3961 tcgacaggcc cgtgcagggc ctctggttcg cctacatggt tggtgccacg gcattctgcc
     4021 tgggcattgt gggtacgctg tggtcgagtg tggctggcgg gcgggggacc cggctgggtg
     4081 gcgtcgtgct gacggcgctg ggtatcggca gcgtcaccgt gtcactggga ttccacggcg
     4141 ggcagggcaa gctcatctct ggcctcatct gcctggcttt cggcctcgta gccatgcggc
     4201 gcgacgatat ggactggggg atcggctgcg gcgccctgtc ggccatgctg ctggcggcgg
     4261 gcgccgtcgc catgatggcc ctgagaggtc ccatgtggac cgtgtttgag ctcaaggatc
     4321 cccgcttctg gcagtacttc tacaacacgg tatatctgat gctcggcatc ccattttcca
     4381 tcgccgggtc gctggcactg gcgctgctcc tcaacgatga gctgaggctc ggcgagtctg
     4441 tgggccgggt catcggcgcc gggctgtgcc tggtgggcgg cgcggtgacc ctcgcggtca
     4501 tctgggagac ctcgcccgat ctcggcctgc tcggggccgt gctctggggg gtggcggctc
     4561 tcgggttcgc ggcgaacgtc gtctccttcc gcaccctgtt ctacctccca tccttcactg
     4621 ccggcgtagc gctgatgatc ctctggaaag ccctctacaa ccccgatacc ggacccatca
     4681 acgtcggact gaaagccgtc ttcgacacct tcggtgtcgc ggccgagcca cccaagtggc
     4741 tggccagcgt cgagtgggcc aagccggcac tcatcatgat gggcgtctgg acgggcatcg
     4801 gcggcaccaa catgctgctc tacctggcgg ggctctcgaa tgtgccccgc gacctactcg
     4861 acgccgctga ggtcgacggc gctgggacat gggcgcgctt ccgccatgtc acctggcccc
     4921 agctcgcgcc gacaaccttc ttcatctcca tcatgtccat catcggcggc ctgcaaggcg
     4981 gcttcgacca ggctcgagtc atgaccggtg gcgggcccgc cgggtcaacg accacgctga
     5041 gctactacgt gtacaacaaa gcattccagg acctggacct cgggtacgcc gcagccatct
     5101 cgtgggtgct gtttgcgatg atcttcgtcg ccacggccat caactggcgt ttcggacgcg
     5161 agctcgaggt gggcccatga ctcgccggaa gctctccaac gccgcccgct tcgtcattct
     5221 gtgcatcatt gggttcagca tctgcatccc gttcgtgtgg atggtcagcg cgtcgttcaa
     5281 gccgcgagcc gaggtcgagg aagtgaacat cgtcccgcac cagcctacga cgcgaaacta
     5341 cccgatcgtg atggacctcg agcccgatcc ggtgacgggc aagaagctca acatgcggtt
     5401 tggccgatgg tatttcaaca gcgttttcat cgccgtgggt gtgaccttcc tgcaggtgtt
     5461 gaccagcgcc atggccgcct atgcgttctc gcgcatccgc tgggccggcc gggataccgt
     5521 gttcctcctc tatcttgcga cgatgatgat ccccggcgtg gtcaccatga tccccaactt
     5581 ccagatcatg gtctcgctag gtttcctgaa cacctaccgg ggattggtcc tccccgcggc
     5641 cttcagtgcc ttcggcacat tcctgctgag gcagttcatg ctctcgatcc cgcggtcgct
     5701 cgacgaggcg gccgagatcg atggcgctcg gcactggcag atcttctggg atgtgatcat
     5761 gcccctggca cgtccggggt tgatcgcgct ggcgatcatt acattcctgg gggcctatca
     5821 gtctttcttc tggccattga tcatgctcaa tgatgcccag ttctacccga tgcccgtggg
     5881 catgttggcg ctcgacagca gctatggacg tcaaacggag ctgattatgg cggccacggt
     5941 catgaacgtg gtgccgctga tcatcatctt cgtgctcttt cagaagttcc tggtcaaggg
     6001 gctgcagctc ggcggagtga aggggtagcg cgatgaagtg ggtcttcgcc ggcgttctgc
     6061 tgctgttcgc gggaatgggg ctcgtggcct gggcgacttt ggtgcctgcg gagacgccgg
     6121 gcgtcgtgcg gattcgctgg gcgacggatg ctaacccggc gagagacgtg cagacgcgtc
     6181 ttttcagtga gatgtacccg ggtgtcgagg tggtggtgga tcccggcctc ggcggcgacc
     6241 agactaagct cattgtccag tgcgccactg gtaccggccc cgatgtcgtc gatgtctaca
     6301 gcaaggagca gatgatggcg ctggtggacg ccggcatcct gctggatctg acgccctatg
     6361 ctgagacgat gggcttcggt gtggacaaga cctaccccgc catggagggt gggctcgttg
     6421 ttgacggccg gcagtaccgg tttccctgca atgtttgggc caactgcgtg gtctacaaca
     6481 agcggatctt cgacgaccac ggagtcccct acccgcggga cggctggacg tgggatgagt
     6541 tcatccgtgt tggtaagcaa ctcaaggaga ccccttcg
//
LOCUS       DAOSPL010000042         6548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_11861_length_6548_cov_3.316803, whole genome shotgun sequence.
ACCESSION   DAOSPL010000042 DAOSPL010000000
VERSION     DAOSPL010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11861_length_6548_cov_3.316803"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PLQ54_01430"
     CDS             <1..268
                     /locus_tag="PLQ54_01430"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91951.1"
                     /translation="EVYDAERVQGYALSWASIHAILARAASVGLEERGRLLRVDPDRA
                     DVIVPGAALALAIIERLPARAVVVDTYGVRLGAILAGTGQEAPA"
     gene            265..1344
                     /locus_tag="PLQ54_01435"
     CDS             265..1344
                     /locus_tag="PLQ54_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOS91952.1"
                     /translation="MMTRRRLIQGLAGAAATRLVPSALAQKGATMVDGSHLPRWRGFN
                     LLEKFTEQHHSAFSEWDFAHIAQWGFDFVRLPMSYRCWASGHPEHWLELDEASLAQVD
                     RAVELGRQYGVHVNLNLHRAPGYCVNPPPEPLSLWADDRALDACAFHWGHLAQRYRGI
                     SNQHLTFDLLNEPGMIEEAVYVSVVERLVEAIHEQDPARLVIADGLAWGRNPVRGLAG
                     LGIAQSTRGYDPMEISHYRASWIGGSDQWPAPTWPLRRGDGSVCDRDWLRRERIQPWK
                     DLEATEVGVHVGEWGAFNRTPHSVVLAWMKDCLELWQEAGWGWALWNFRGSFGLLDSG
                     RDDVAYEDVDGHRLDRAMLELLRAH"
     gene            1414..3702
                     /locus_tag="PLQ54_01440"
     CDS             1414..3702
                     /locus_tag="PLQ54_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91953.1"
                     /translation="MQRMWWTIPGVALLAGLSVAPAPGTGGPEQYRVSDGVMPDVGCW
                     FWTEREFEPDGYRDFIDLVAAHAPYKLLTTSIRAPLVEVTDREVHDQVAAAVRYARRF
                     GIGVVMDLDVRLARQAFRRAHPDELQEMLRIRPVVFGADGPGEVCIESTDLNDHYTGR
                     TTHYVPLSGRLVRVYAARGPAGEITADALVDVTGRCQVVEATEARVVVRAPREVHPEG
                     GSVYALVAFTHLTPDVFAPSLLEFEANTLRSYADVPLAGVCKDEWGFPPCFETQPAHN
                     DYWYSEHLASAYSRETGGRDLVRDCLLMYAGDGRGEAGRERARAINVLMRMVWQRNAE
                     IEDAYFRTIKAVFGSKALSATHPTWFPYPDVREVHKNGLDWWAATRDLAQTDEITPFS
                     CRTALSKKWGSSVWLNMYYSTDYEEYVRELWAGALGGGRVNYHPLCPREAPVFDSSAA
                     LLRGGLMRGQCRVRLLNYISRSPLDCPVAVVFGHAAALNWSTPAYGDAGVGVANALWE
                     AGYPADLIPTSEIATGALSIARSGRLRYGAQEYAAAILYHPEFEPLATGRFLDRVARR
                     GATRVAWVGPWTMGPDGAPLDGKAGLSSVGGSEDVDSCVRRTIEALLAAGVPPQTRAI
                     AETRWGLPVAIPPREGSARLLDRTHIVVSAAREAGGDTIRGTVALGPHAVDVEAVGLV
                     AARWRDDGVLEAMAAGGLRRFVTEGFVLELREPVDLALWRDESGHWRGVVQGLDGPPP
                     AELLALTRRWGRLSVPPPVPPM"
     gene            3733..6222
                     /locus_tag="PLQ54_01445"
     CDS             3733..6222
                     /locus_tag="PLQ54_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOS91954.1"
                     /translation="MLHRTAVCRLGPPLVGMVLCAAVGAVQDNPMLARYQRAERMGSA
                     ELGRLVHKARVEPHWIHGTARFWYRNDVRGDRDFVVVDAEAGTRGPAFDHQRVAKALT
                     RVTGQACRAERLPFRGVEFVANGSAIEFRIGDQLWRCDLATYDCTRSGTTALPSRQPV
                     EEPQAEDGRSPDGRWVAFARDGDLWLRLRDTGEECRLTTDGTASDYYGAPQWSPDSGT
                     LVAWRTVPGERLPMYVIDSAPEGSLRPRLDVYEYELPGDRVDVHRLFVFDVATRLGRA
                     VNTDPVSWGGPPEVRWSPDGTSFTFEHTDRGYQRVRVIEVDPALASSRTIIEERSSTF
                     IAPMKQITRYLDATGEIIWSSERDGWNHLYLYDARAGALRTQITRGEWVVRDVVRVDE
                     SARTLVFAACGRELGQDPYLIHYYRVGLDGSGLTCLTPGDGHHEVRFSPDGRFLIDTY
                     SRVDRAPVSELRRTADGSLVMVLEEADVADLLAEGWRWPEPFVAQGRDGATDIWGVIF
                     RPTDLDESRQYPVIEMIYAGPQDSFVPKTFAAARAQQELAELGFIVVQIDGMGTSNRS
                     KAFHDVCYRNLGDGGFPDRIAWLRAAAERYPYMDLSRVGIIGHSAGGYNAARALLAFG
                     DFYKVAVAESGNHDHRTDKAWWNELWMGYPVGPHYAEQSNVTQAARLSGKLLLIHGEI
                     DHNVNPWASTMRLADALIAANKDFDLLILPGNDHGYQGHYAKRRAYDYFVRHLLGVEP
                     PKEYSFGGAADAGAECTVTIRNTLDEPVGIYWITFEGDLRKYHDLEPGQEVRQHTYVG
                     HLWEAQTGAGTVSRYTASTREPVWEVGREAE"
BASE COUNT          974 a         1998 c         2427 g         1149 t
ORIGIN      
        1 ggaggtttat gacgcggagc gggtgcaggg ctatgcgttg agttgggcct ccatccatgc
       61 aatcctggct cgtgccgcgt cggtgggact ggaggagcgc ggccggcttc tgcgcgtcga
      121 cccggaccga gcggacgtga tcgtgcccgg tgcggcacta gcccttgcga tcatcgaacg
      181 cctgcccgcc cgggccgtgg ttgtggacac ctacggcgtc cggctggggg cgatcctggc
      241 cggcaccggt caggaggcac cggcatgatg acgcgacggc gtctgatcca gggcctggct
      301 ggcgccgcgg cgacccgcct ggtgccgtcg gctctggctc agaagggagc gaccatggtg
      361 gatggcagcc atctgccccg ctggcggggc ttcaacctgc tggagaagtt caccgagcag
      421 caccactcgg ccttctcgga gtgggacttc gcccacatag ctcagtgggg tttcgacttc
      481 gtccgcctgc ccatgtcgta tcgctgctgg gcttcgggcc accccgagca ttggctggag
      541 ctggacgagg catcgctggc gcaggtggac cgggcggtgg agctgggaag gcagtacggc
      601 gtccatgtga acctgaacct ccaccgggcg ccgggctact gcgtgaaccc gccgccggag
      661 ccattgagcc tatgggcgga cgaccgggct ctggacgcct gcgcgttcca ctggggccac
      721 ctggcgcagc gctatcgggg catctcgaat cagcacctga cctttgatct gttgaacgag
      781 ccggggatga tcgaggaggc cgtgtacgtt agcgtggtgg aacggctcgt ggaggccatc
      841 cacgagcagg atccggcgcg tctggtcatt gccgacggtc tggcctgggg gcgcaacccg
      901 gtgcggggtc tcgccggtct ggggatcgcg cagagcactc ggggatacga cccgatggag
      961 atcagccact atagggcctc gtggatcggc ggctcggatc agtggcctgc gcccacgtgg
     1021 ccgctccggc gtggtgatgg cagcgtgtgc gatcgcgact ggcttcggcg cgagcgcatc
     1081 cagccgtgga aggatctcga ggccaccgaa gtcggtgttc atgtcggcga atggggcgct
     1141 ttcaaccgaa cgccccattc ggtcgtgctg gcctggatga aggactgcct cgagctgtgg
     1201 caggaggccg gctggggctg ggccctgtgg aactttcgcg gaagcttcgg gctcctcgat
     1261 agcgggcggg acgacgtcgc atacgaggat gtcgacgggc accggctcga ccgagcgatg
     1321 ctggagttgc tgcgggccca ctgagtgcgt ggcggcgggg aaccgcgagc gtctggcgcg
     1381 aactcccgtt cccttggcgg agaggagacg atcgtgcagc ggatgtggtg gaccatccct
     1441 ggagtggcgc tgttggctgg cctgagcgtt gctcccgcgc ctggtaccgg aggacccgag
     1501 cagtaccgcg tgtctgacgg ggtcatgccc gacgtgggtt gttggttctg gacggagcgc
     1561 gagttcgagc ccgacgggta ccgggatttc atcgacctgg tcgccgcgca cgcgccctac
     1621 aagctgctca cgaccagcat ccgtgcgccg cttgtggagg tgaccgaccg cgaggtgcac
     1681 gatcaggttg cggcggcggt tcgctatgcg cggcggttcg gcatcggcgt tgtgatggac
     1741 ctggatgtgc gcctggcgcg acaggccttc cggcgggctc atcccgacga gctgcaggag
     1801 atgctgcgga tccgcccggt ggtgttcggt gccgacggcc cgggcgaagt gtgcatcgag
     1861 agcacggacc tgaatgatca ctacacggga cggacgacgc actatgtccc gctgtcgggc
     1921 cgtcttgtgc gggtgtacgc ggcccggggc ccagctggtg agatcacggc cgacgcactg
     1981 gtcgacgtga ccgggcggtg ccaggtggtc gaagccacgg aggcgcgagt cgtcgtccgg
     2041 gcaccgcgtg aggtgcaccc cgagggtggt tcggtctacg ccctggtggc gttcactcat
     2101 ctgacgcccg acgtgttcgc cccgagcctc ctcgagtttg aggcgaacac cctgcggagt
     2161 tacgccgacg ttccgttggc cggggtttgc aaggacgaat ggggctttcc gccctgtttc
     2221 gagacgcagc cggcgcacaa cgactactgg tattcggagc acctggcctc tgcctactcg
     2281 cgagagaccg gagggcgaga tctcgttcgc gactgcctgc tgatgtacgc cggggatggc
     2341 aggggcgagg ctggccgtga gcgcgcacgg gccatcaatg ttctcatgcg gatggtctgg
     2401 cagcggaacg cagagatcga ggacgcctac ttccggacca tcaaggcggt ctttggttcg
     2461 aaggcactgt cggcgacgca tcccacctgg ttcccctacc ctgatgtccg cgaggtacac
     2521 aagaacgggc ttgactggtg ggcggcgact cgcgatctgg cgcagacgga cgagatcact
     2581 cccttctcct gccgcacggc gctgtcgaag aagtggggga gctcggtctg gctcaacatg
     2641 tactactcga cggactatga ggagtacgtt cgcgagttgt gggccggagc gctgggtggc
     2701 gggcgggtga actaccaccc gctgtgcccc cgcgaggcac cggtgttcga cagctcggcc
     2761 gccctgctgc gcggtgggtt gatgcgcggg cagtgccggg tgcgtttgct caactacatc
     2821 agcaggagcc cactggactg ccccgtggct gtggtcttcg gccatgccgc ggcgctcaac
     2881 tggagcacgc cggcctatgg cgacgccggc gtgggggtag cgaacgcgct gtgggaggcc
     2941 gggtacccgg ccgacctgat cccgaccagt gagatcgcga cgggcgcgct gagcatcgcc
     3001 aggtccggcc gactgcggta tggtgcccag gagtacgcgg ctgccattct gtaccacccc
     3061 gagtttgagc cgctggccac tggacggttc ctggatcggg tggcccgacg gggcgcaacg
     3121 cgcgtggcct gggtcggtcc gtggaccatg gggcccgatg gcgccccgct tgacggcaag
     3181 gccggcctgt catcggttgg cgggagcgag gatgtggatt cgtgcgtgcg ccgaaccatc
     3241 gaggcccttc tggcggccgg ggttccgccg cagacccgcg ccatcgccga gactcggtgg
     3301 ggtctgccgg tggccattcc gccgcgcgaa ggctcggcgc gtctgctgga tcgcacgcac
     3361 atcgttgtct cggcagcgcg agaggccggt ggcgacacga tcaggggaac cgtggctttg
     3421 ggtccgcacg cggtcgatgt cgaggccgtc ggcctggtcg cggccaggtg gcgcgatgac
     3481 ggggtgcttg aggcgatggc cgcgggcggg ctgcggcggt ttgtcaccga gggattcgtc
     3541 ctcgaacttc gtgaacccgt tgacctggcg ctgtggcgtg acgagtccgg gcattggcgc
     3601 ggcgtggtcc aggggctcga tggcccgccc cctgccgagc tcctggcgtt gacccgccgc
     3661 tggggtcggc tcagcgtccc gccacctgtt ccaccgatgt agtcgcacgc ccggcgctgc
     3721 aggaggccgt tcatgctcca tcgcacagcg gtctgccgcc tgggtccgcc gctcgtgggc
     3781 atggtcttgt gcgccgcggt gggcgcggtt caggacaacc ccatgctggc tcggtaccag
     3841 cgggcggagc gcatgggctc tgccgaactg gggcgcctgg tgcacaaggc gcgtgtggag
     3901 ccgcactgga tccatggcac tgctcggttc tggtaccgca acgacgtgcg cggcgaccgc
     3961 gatttcgtcg ttgtcgacgc cgaagccggg acccggggcc ccgctttcga ccaccaacgc
     4021 gtcgccaagg cgctcacccg ggtgacgggt caggcatgta gggccgagag gctccctttc
     4081 cgtggcgtgg agttcgtcgc gaatggttcg gccatcgaat tccggattgg ggatcagctc
     4141 tggcgctgcg acctggccac gtacgactgc acccggtccg ggactacggc cctcccgtca
     4201 cgacagccgg tggaagaacc ccaggccgag gatgggcgat cgcccgacgg gcgctgggtg
     4261 gcctttgcgc gtgatggcga tctgtggttg cggttgaggg acacgggcga ggaatgtcgg
     4321 ctgaccacgg atggcaccgc gagcgactat tacggtgcgc cgcagtggtc gccggattcc
     4381 gggacgctgg tcgcgtggcg gaccgtgccg ggcgagcgcc tgccgatgta cgtcatcgat
     4441 tcggcgccag agggcagcct caggccgagg ctagatgtgt atgagtatga actgcccggt
     4501 gaccgcgtgg atgtgcatcg gctctttgtg ttcgacgtgg cgacgcggtt gggacgggcc
     4561 gtgaacactg atccggtgag ctggggcgga ccgccggagg tgcggtggag cccggacggc
     4621 acgtccttca cattcgagca cacggatcgt gggtaccagc gggttcgggt gatcgaggtc
     4681 gatcctgccc tggcctcgtc gcggacgatc atcgaggagc ggagttcgac cttcatcgcc
     4741 ccgatgaagc agatcacgcg ctacctcgac gcgacgggcg agatcatctg gtcgtcggaa
     4801 cgcgacggct ggaaccacct gtacctctac gacgcccgcg ccggtgcgct caggactcag
     4861 atcacgcgag gcgagtgggt ggtgcgcgac gtcgtccgag tggatgaatc ggcacgaacg
     4921 ttggtgttcg ctgcgtgcgg gcgcgagttg gggcaggacc cgtacctgat ccactactac
     4981 cgcgttgggc tggacggatc gggactcacg tgcctcaccc cgggcgacgg ccaccacgag
     5041 gtgcgcttct cacccgacgg ccgcttcctc atcgacacct attcccgcgt cgatcgtgcg
     5101 ccggtgtcgg agctacggcg gacggccgat gggtcactgg tgatggtgct cgaggaagca
     5161 gatgtggccg atctgcttgc ggagggctgg cgttggccgg agccgttcgt ggcccagggc
     5221 cgggacgggg ccacggacat ttggggcgtc atctttcggc cgaccgatct ggacgagtcg
     5281 aggcagtatc cggtcatcga gatgatctat gccggccctc aggactcatt cgtgccgaag
     5341 acctttgccg cagcgcgggc gcagcaggag ctggccgagc tggggttcat cgtcgtccag
     5401 atcgacggca tgggcacctc gaatcggtcg aaggccttcc acgacgtgtg ttacaggaac
     5461 ctgggcgatg gaggcttccc ggaccggatc gcgtggctga gagcggcggc ggaacggtat
     5521 ccctacatgg acctgtcgcg cgtggggatc atcgggcact cggcgggcgg ctacaacgcc
     5581 gcgagggcac tgttggcctt cggcgacttc tacaaggtgg ccgtggccga gtcgggcaac
     5641 cacgaccatc gcacggacaa ggcatggtgg aatgagctct ggatgggcta cccggtgggt
     5701 ccacactacg ccgagcagtc gaacgtgacg caggcggcac ggctatcggg taagctgctg
     5761 cttattcatg gggagatcga tcataacgtc aatccctggg cgtcaacgat gcggcttgcg
     5821 gatgcgctca tcgcggcgaa taaggacttc gacctgctga tcctgcccgg caacgaccac
     5881 ggataccagg ggcattatgc caagcgccgg gcgtacgact acttcgtccg gcacctgctc
     5941 ggggttgagc cgcccaagga gtattcgttt ggcggggcgg ccgacgctgg cgccgagtgc
     6001 acggtgacga tccggaatac cctcgacgag ccggtgggca tctattggat caccttcgag
     6061 ggtgatctgc gcaagtacca tgacctcgag cccgggcagg aggttcggca gcacacttac
     6121 gttggtcacc tgtgggaggc acagacggga gccggtaccg tgtcgcgcta cacggcgtca
     6181 acgagggagc ccgtgtggga ggtcggacgg gaggcggagt agcgggcccg tcgaccggga
     6241 accgccgggt tcgatggcgg ggacagaggg gacccgccgc cggctggtgg agcctgatcc
     6301 ggccctgagc gtgccgtggc ctgcgggctt ccggttgggc cggatggcgc ccttcgcgct
     6361 ccgcgggccc cttccattcg gcgcctgggt tagctcgaat gggacagagc gcctcgctgc
     6421 gtgactcgac ttgtggggcc acagcagtgg ggatgctggc tcgcgcggca ttgggtggag
     6481 tgtgctgtac aaaccacgaa gccccgccag gcggggcttc gtgcgtcaat gatcgtttct
     6541 ggagcgcg
//
LOCUS       DAOSPL010000043         6502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_11958_length_6502_cov_2.997053, whole genome shotgun sequence.
ACCESSION   DAOSPL010000043 DAOSPL010000000
VERSION     DAOSPL010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11958_length_6502_cov_2.997053"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>389)
                     /locus_tag="PLQ54_01450"
     CDS             complement(<1..>389)
                     /locus_tag="PLQ54_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91955.1"
                     /translation="LGHFFMAPHHQWCYTQEKGTHWYEGPTEEYAWLFRAVRDHASLF
                     DGYETVADVGLLVAAGAYREGKHEAEEAAAALTRANIPYRVFLAGDDALPWTLSVDEL
                     RSVRTLVIPHREALNAVDRAELDARAA"
     assembly_gap    390..399
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<400..1399)
                     /locus_tag="PLQ54_01455"
     CDS             complement(<400..1399)
                     /locus_tag="PLQ54_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91956.1"
                     /translation="MLSLAWPLFAAAVGRGDPAPLPVLRHSDVVFMYEASADVYRAYG
                     ATVLAWGGTPTAEALATAHGAGVAYFGSVGMVTEFGRVIDAYPGYEEAICLDVEGNRL
                     RVPWLWDHQHKGVPAYWFCTNQPIFRDYMRRRVVETVRAGADDVHIDDHLGTAGNSWL
                     GGCYCDQCLAGFREYLAVDAPPDDLAGAGIADPAGLDYRALVLGFIAQHPEAKEQLWR
                     RPLAHHFATFQSRRAAAFMMELRRLAADTAGRPLPMSANAGVPHIAHLSDYRALDFLS
                     CEVEQRASELKPYDGVAFAYRMAEALGRPVAGTASGWDWSVINDKRLSGLVRLWIAQ"
     gene            complement(1441..1608)
                     /locus_tag="PLQ54_01460"
     CDS             complement(1441..1608)
                     /locus_tag="PLQ54_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91957.1"
                     /translation="MDDAVEQCPECGYTGGFHVVVERRSQTSDTNALLLLRCPGCRAT
                     FDIGWVGSLVG"
     gene            complement(1655..2725)
                     /locus_tag="PLQ54_01465"
     CDS             complement(1655..2725)
                     /locus_tag="PLQ54_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOS91958.1"
                     /translation="MSEPMDSRVRTVLDGGTVIPAVPLALTAERRLDERRQRALLRYY
                     VAAGAGGLAVGVHTTQFAIRRPEIGLFEPVLALAADEMSHLDAARSGPLVRIGGVCGP
                     TPQALGEAECLRRLGYHAALLSLAALRDADDDVLLAHCRAVAGVMPLVGFYLQPAVGG
                     RVLSHAFWRRFCEIENVVAIKIAPFHRYRTLDVVRAVAEVGRDDIALYTGNDDSIVVD
                     LLTPYRVRVGACEVERRIVGGLLGHWSVWTLRAVELLDRCHRAVRHGGDAPVDLLRLA
                     GQVTDANAAVFDAANGFAGCIAGIHEVLRRQGLLAGSWCLDPNEGLSPGQAEELDRVH
                     AAYPHLNDDGFVAAHLDEWLAD"
     gene            complement(2741..4291)
                     /locus_tag="PLQ54_01470"
     CDS             complement(2741..4291)
                     /locus_tag="PLQ54_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOS91959.1"
                     /translation="MVGSAALPLSVLWVLAASPADSVPYTLWAAPSVQKVFRDAEPSG
                     LGEPAHIYAAANEFEAVQALLTAGTESLEDVTVRVMPFRRERHAIGSASVTLYLAHYV
                     PCPATGKDYPDPLPPLRDGFDVPAGTSQPIWIEVFVPEGTPAGDYAGGIVVTPANAPG
                     RRLPLRLTVYGFELPDQVHAVTAFGISDDCVFDKHGVASSDGQATNARARLRQRYYEF
                     LLEHKISPYYLPVDLRSPWASKYLNDPRLTSFAVPYREDEGYLRDIAAYLRERGLLKK
                     AYFYVVDEPVARPSYDEIRHVAEVLHRVDPQLRLVAPFYRAPDWDESQTPFDELTGYL
                     DIWCPNAHYFDLNPRVHQQIQERRAAGETAWCYVCCGPGEPYNNFFVQMSGLSHRLLF
                     TQLWRLGVEGLLYWSTTYWAAVADPWTDMTTVPDINKSIYGDGSLLYPGKPVGIDGPV
                     ASIRLKIIRDGIEDYEYLRLLAQRRGMLAAHRAAEALCPSLSQYTRDPATFAFLRHKI
                     AREIEPDR"
     gene            complement(4331..5293)
                     /locus_tag="PLQ54_01475"
     CDS             complement(4331..5293)
                     /locus_tag="PLQ54_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91960.1"
                     /translation="MPHPVFDRSRLRLRPLFERVHDFTHAQVIPLERPAPAFDDPQIE
                     ALSVRIRAARQTGAPVILSMGAHVLKVGLSLYVADLVRQGWVTALALNGACVIHDFEM
                     ALIGATTESVAHYIQQGQFGLWTDTGRINALAGEAAAEGIGLGERVGREIATGDFPYR
                     HLSILAAAYESQVPATVHVGIGYDIIHEHPNCDGAALGAASYHDFLVFAEVVRHLEGG
                     VFLNYGTAIMGPEVYLKALSMARNVAHQEGKEIRHFTTAVFDLIDLGNRLDTEAPKSD
                     PKYYYRPFKTVLVRTVADGGESFYIRGDHTATLPNLWRLLTRQA"
     gene            complement(5327..>6502)
                     /locus_tag="PLQ54_01480"
     CDS             complement(5327..>6502)
                     /locus_tag="PLQ54_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOS91961.1"
                     /translation="GRWVVARLAGVGDVRPEPTVVRLLADRQPGVALASHWRPTSPIG
                     FTPAAHPVADSHAAPDHGLMTAAFNLERWLSIPPLPRSRRAKPPGAGQLTTALTPHTI
                     IRGRSLCAKDDTMPERAYLPGTSIELCNPAIPQGQIRFCLFDFDGTLSLIREGWQDVM
                     VPMMVEVLSGTPRAESREALFGVVRDYVDRLTGKQTIYQMLQLVEEVKKRGGTPKDAL
                     AYKHDYLALLWERIQHRVADLKAGRAQPDEYLVAGSVKLLTGLRDRGVTLYLASGTDM
                     PCVLDEAEALGLTGYFDGGIYGALDNWESFSKRMLIQDLVQRFQLRGPQFLTFGDGFV
                     EIENCKEVGGIAVGVASDEARRSGVDEWKRNRLIGAGADMIIPDFREADALLGYLFP"
BASE COUNT         1197 a         2140 c         2142 g         1013 t
ORIGIN      
        1 cggcggcccg ggcatccagc tcggcgcggt ccacggcgtt cagtgcctct cggtgcggga
       61 tcacgagtgt cctcactgac cgcagctcgt ccacagaaag ggtccagggc agggcatcat
      121 cgccggccag gaacacgcgg tacgggatgt tggcgcgggt cagcgcggct gccgcttcct
      181 cggcctcgtg cttgccctca cggtacgcac cggccgcgac caacaaaccc acatcggcca
      241 cagtctcgta gccgtcgaac agcgaggcgt ggtcccgaac ggcgcggaac aaccaggcgt
      301 actcctccgt cggcccctcg taccagtgag tgcccttctc ttgcgtgtag caccactggt
      361 gatggggcgc catgaagaag tgccccaggn nnnnnnnnnc ctgcgcgatc cacagacgga
      421 cgaggccaga gagtcgcttg tcgttaatga ccgaccaatc ccacccgctc gcggtacctg
      481 cgaccggacg ccccaacgcc tcggccattc tgtaggcgaa cgccacaccg tcgtagggtt
      541 tgagctcact cgccctctgt tccacctcgc acgacaggaa atcgagcgcc cgatagtccg
      601 aaaggtgagc aatgtggggg acaccggcgt tcgccgacat gggcagggga cgcccggccg
      661 tgtcggcggc gagccgcctg agctccatca tgaacgccgc cgctctccgg ctctggaagg
      721 tggcgaagtg gtgggcgagc gggcgtcgcc agagctgctc cttggcctcg gggtgctggg
      781 cgatgaaccc gagcacgaga gcccgatagt cgagtcccgc cggatcggca atgccggcgc
      841 cagccagatc gtcaggcgga gcatccactg ccagatactc gcggaagccg gccaggcact
      901 ggtcgcagta gcaaccgccg agccagctgt tgccggccgt tccgagatgg tcgtcaatgt
      961 gaacatcgtc ggcaccggcc cgaaccgtct cgacgacccg gcggcgcatg tagtcacgga
     1021 agatcggctg gttcgtgcag aaccagtaag ccggcacgcc cttgtgctgg tgatcccaca
     1081 accacggtac ccgcaggcgg ttgccttcga catcaaggca gatggcctct tcatagcctg
     1141 ggtaggcgtc gatcacccga ccgaactccg tgaccatgcc cacactaccg aagtaggcca
     1201 ctccggcgcc atgcgctgtg gcaagcgcct ctgctgtggg ggtccccccc cacgcgagaa
     1261 ctgtggctcc gtaggcgcgg tacacgtccg cgcttgcctc atacatgaac acgacatccg
     1321 agtgcctgag caccggaagg ggggccggat cgccgcgccc aaccgcggcc gcgaacagcg
     1381 gccatgcgag agagagcatc ccgaacccct ccgatcgccc ctgcccggct ccgggtggca
     1441 ctagcccaca agcgagccga cccagccgat gtcaaatgtg gcccggcatc caggacagcg
     1501 aagcagcagg agcgcgttcg tgtcactggt ctgcgaccgg cgctccacga cgacgtgaaa
     1561 cccccccgtg tagccgcact cggggcactg ctccaccgcg tcgtccacgg gtagcttctt
     1621 gtgctcgatc agcgtggttc atctcctcgg tagtctagtc tgccagccac tcgtcaaggt
     1681 gggccgccac aaagccgtca tcgttgaggt gagggtaggc ggcgtgcaca cgatccagtt
     1741 cctcggcctg cccggggctc aggccctcgt tggggtcgag gcaccaggag cccgcaagca
     1801 gcccctgtcg ccgaagcact tcgtggatgc ccgcaataca gcctgcgaaa ccgttggcgg
     1861 catcgaacac tgcggcgttg gcgtctgtaa cctggccggc cagcctcagg aggtcgaccg
     1921 gggcatctcc gccatggcgg accgcccggt ggcaccggtc gagcagctcc accgcgcgga
     1981 gcgtccacac cgaccagtgg cccaaaaggc cgcccacgat gcgtctctcg acctcgcacg
     2041 cccccacccg gacccggtat ggcgtcaaga ggtcgaccac gatgctgtca tcgttgcccg
     2101 tgtagagtgc gatatcatct cgaccgacct cagccacggc ccgaaccaca tcgagcgttc
     2161 ggtagcggtg gaatggcgcg atcttgatgg ctactacgtt ctctatctcg cagaatcgac
     2221 gccagaaggc gtgtgagagg acccggccgc ccacggccgg ctgcaggtag aagcccacca
     2281 gcggcatcac accggccacg gcacggcagt gggcgagcag gacatcgtcg tccgcgtcgc
     2341 ggagcgccgc caggctcaac agcgcggcat ggtacccgag cctccgcagg cattcggcct
     2401 cacccaacgc ttgcggcgtc ggaccacaca cgccgccgat tcgcaccaga ggcccagagc
     2461 gggcggcgtc caggtgagac atctcatcgg ccgccaatgc gagcacgggc tcgaacagcc
     2521 caatctcggg cctgcggatt gcgaactgcg tcgtgtggac gcccaccgcc agcccgccag
     2581 cgcctgcggc cacgtagtac cgcagaagcg cgcgctgtcg ccgctcgtcc aatcggcgtt
     2641 cggccgtgag cgccagggga actgccggga tgacggtccc tccgtcgagc accgtgcgga
     2701 cacgcgaatc catcggttcg ctcatgggtc ctcccaggcc ctaacggtcc ggttcgatct
     2761 ccctcgcgat cttgtggcgc aggaacgcaa acgtcgcggg gtcgcgggtg tactgcgaga
     2821 gcgatgggca gagcgcttcc gccgcccggt gggcggcgag cataccccgc ctctgagcga
     2881 gcaaccggag gtactcgtag tcctcgatcc catcccggat gatcttcagc cgaatcgagg
     2941 ccacggggcc gtcgattccc acgggcttgc ccggatagag cagcgaccca tcaccgtaga
     3001 tgctcttgtt gatgtccggc acagtggtca tgtccgtcca cggatcggcc acggctgccc
     3061 agtaggtggt gctccagtac agcagaccct cgacgcccag ccgccagagc tgggtgaaga
     3121 gcagtcgatg cgagaggcca ctcatctgga cgaagaagtt gttgtacggc tcgcccggcc
     3181 cacagcacac atagcaccac gcagtctcac ccgcagcccg gcgctcctgg atctgctggt
     3241 ggacgcgcgg gttgaggtcg aagtaatgcg cgtttgggca ccagatgtcg aggtacccgg
     3301 tcagctcgtc gaaaggggtt tgggactcgt cccagtcggg tgcacggtag aacggggcca
     3361 ccagacgcaa ctggggatcc acccgatgaa gcacctctgc aacatgtcgg atctcgtcat
     3421 atgaggggcg cgcaactggc tcatcgacca cgtagaagta ggccttcttc aggagacctc
     3481 tctcgcgaag gtacgccgcg atgtccctga ggtacccttc gtcttcgcgg tagggcacgg
     3541 cgaaggaggt cagacgaggg tcgttgaggt acttcgaggc ccacggtgac cgcagatcga
     3601 ccgggaggta gtagggcgag atcttgtgct ccagtagaaa ctcatagtaa cgctggcgaa
     3661 ggcgggcccg agcgttcgtg gcttggccat cggagctggc cacaccgtgc ttgtcgaaga
     3721 cgcagtcatc gctgatcccg aaagccgtga ccgcgtgaac ctgatcgggc agctcgaagc
     3781 cgtagacggt gagccgcaac ggcaggcgcc tgcccggggc gttggccggg gtgacgacga
     3841 tgcctccggc ataatcgccg gccggagtgc cctcgggcac gaacacctcg atccagatgg
     3901 gctgacttgt gccggcgggc acatcgaacc cgtcacggag gggtgggagg gggtcggggt
     3961 agtccttccc ggtggcgggg caggggacgt agtgggcgag gtagagggtc acacttgcgc
     4021 tgccgatggc atgacgttcc cggcggaaag gcattacgcg gacagtcacg tcctccaacg
     4081 actcggtacc ggcggtgagg agggcctgaa ccgcctcgaa ctcgttcgcc gcggcgtaga
     4141 tgtgggcggg ctcccccagc cccgacggct cggcatcacg gaagaccttc tggacgctcg
     4201 gcgccgccca aagcgtgtat ggcaccgagt cggccggtga ggcggccagc acccacagga
     4261 cggagagcgg gagagcagcg ctgccgacca tcgcgacgtc acctccgaaa gcctctgaag
     4321 cgcgaaaaac tcaggcctga cgcgtcagca ggcgccacag gttgggcagg gtggccgtgt
     4381 ggtcaccacg aatgtaaaag ctctcgccac cgtcggctac cgtgcggacg agaacggtct
     4441 tgaacggccg gtagtaatac ttcggatcgg atttcggggc ctccgtatcg aggcgattgc
     4501 ccaggtcaat caggtcgaag acggctgtgg tgaaatggcg gatctccttg ccctcctggt
     4561 gggcaacgtt ccgcgccatt gagagggcct tgaggtacac ttccggcccc atgatcgcgg
     4621 tgccgtagtt gaggaagaca ccgccctcga ggtgccgaac cacctcggcg aaaacgagga
     4681 agtcgtggta cgaggcggcg cccagagccg caccatcgca gttcgggtgc tcgtgaatga
     4741 tgtcataacc aatccccaca tgaacggtgg ccggtacttg gctctcatag gccgcggcca
     4801 gaatgctcag atggcggtag gggaagtcac ccgtggcgat ctcgcggccc acacgttcac
     4861 ccaggccgat gccctccgcg gcagcctcac ccgccagagc attgatgcgc cccgtgtccg
     4921 tccagagccc gaactggccc tgctggatgt agtgggcgac gctctcagtc gtggcgccga
     4981 tgagagccat ctcgaagtcg tggatcacgc acgcgccgtt cagggccaga gctgtgaccc
     5041 agccctggcg caccaggtcg gccacgtaga gcgataggcc gaccttgagc acgtgcgcgc
     5101 ccatagagag aatgaccgga gcgccggtct gccgggccgc ccgaatgcgc accgacaggg
     5161 cctcgatctg tgggtcatcg aaggccggag ccggccgctc gagcgggatg acctgcgcgt
     5221 gggtgaagtc atggacacgc tcgaagagcg gacgcagccg cagacggctc cgatcaaaca
     5281 caggatgggg cacgagggac acctccggcg ccacgggaat gaaatcctag ggaaacaggt
     5341 agccgagtag cgcgtcggcc tcgcgaaagt ccggtatgat catgtcggct cccgccccga
     5401 tgagccggtt gcgtttccac tcgtccacgc cggagcggcg agcttcgtcg gacgccacgc
     5461 ccacggcgat cccgccaact tccttgcagt tctcgatctc gacgaagcca tccccaaatg
     5521 tcaggaactg cgggccgcgc agctgaaaac gctgcaccag gtcctgtatc agcatgcgct
     5581 tcgagaagct ctcccagttg tccagggcac cgtagatgcc accatcgaag taccccgtga
     5641 gaccgagggc ttcggcctca tcgaggacgc acggcatgtc ggtgccgctg gcaaggtaga
     5701 gcgtaacacc gcggtcgcgg agccccgtga ggagcttgac cgaacccgcg accaggtact
     5761 catcaggttg ggctctgccc gccttgaggt ccgccacgcg gtgctggatg cgctcccaga
     5821 gcagggccag atagtcatgc ttgtaggcga gagcgtcctt gggtgtgccc ccgcgcttct
     5881 tcacttcctc cacgagctgc agcatctggt agatcgtctg cttgccggtg aggcggtcca
     5941 cgtaatcgcg gaccacgccg aacagggcct cgcgagactc ggctctcggg gtccccgaaa
     6001 gcacctcgac catcatgggc accataacat cctgccagcc ctcgcggatg agagagagcg
     6061 tgccgtcgaa gtcgaacaga cagaagcgaa tctggccctg cgggatagcc gggttgcaca
     6121 gctcaatgga cgttccgggg aggtacgcgc gctcgggcat ggtgtcatcc ttcgcacaca
     6181 ggctgcggcc gcggatgatg gtatgggggg tcagagccgt cgtcaactgc cccgctccgg
     6241 gcggcttggc tcggcgtgag cgcggcaggg gcgggatcga caaccatcgc tcgaggttga
     6301 acgcggccgt catcaggccg tggtcaggcg cggcgtgcga atcggccaca gggtgagcgg
     6361 cgggggtgaa gccgatgggt gatgtgggcc gccaatgtga ggcaagggcc acaccgggct
     6421 gccggtcggc aagaagacga accacggtcg gctcgggccg aacgtcgccc acacctgcga
     6481 gacgggccac gacccagcga cc
//
LOCUS       DAOSPL010000044         6496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_11967_length_6496_cov_3.464214, whole genome shotgun sequence.
ACCESSION   DAOSPL010000044 DAOSPL010000000
VERSION     DAOSPL010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11967_length_6496_cov_3.464214"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PLQ54_01485"
     CDS             complement(<1..662)
                     /locus_tag="PLQ54_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOS91962.1"
                     /translation="MARRGFTLVELLVVVAIIAILVAILFPVFARVREKARQTSCLNN
                     LKQIGMALEQYTADSGGRWPQNDPLSPNDARAFRYNCTYPGWIINAPLEPYERSPEIY
                     HCPTRSNGMFNNWRNNLKPISYGYNYVGLYGESEQSIYQPVKFLVMWDSSNPWADSFM
                     SIWSRDVAWFLNDTFRNTSWHNGVNNYLFSDGHAKAMKWSQVTWDQIWNSRYVGLNHP
                     DWG"
     gene            complement(883..2466)
                     /locus_tag="PLQ54_01490"
     CDS             complement(883..2466)
                     /locus_tag="PLQ54_01490"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOS91963.1"
                     /translation="MAWSLAGAACVGLQWVQPAASVGCVRALGAVLLVASGALICLPM
                     STWLPLSAVALLTLASALSGAGPEAAWLGVAACLAWLALAEGWTRQGLPLQGWATALR
                     AAGSATAVGLLVAVARGEWPAVAWYRELGYGQRPWDVPWVPGQPAALALVAVALPLAV
                     DSWATAGAASARRLGWLELAVSTAALMLAGGWLGWLALGAAIVASAGWRACWRWPQVG
                     IGVGALVLVCVAALPVRHSWMPHVQPLLASIREEVQLRVYAGHAAWRLLSQRPGLGWG
                     PGSFGAVYGPARPGPIAEPFQAVERPATAPSDLLLCLVETGVPATLLVVALCVPACAG
                     CRGHGGPHVPRGWRAALVSSLGVSAIGGGAAWPLAAASVMVTVGVIRGMRQGRSRSGP
                     RVHCSWPRLGLAAVVGLVMAMSPAAEWASPARRGSGTGDPRSPEMLLDRACAVAPRNP
                     EAARSLLLEALRVFPLREEAARRAGRGDRQRWGEVRLAHIAAADEMAVLSASLGASEA
                     GRAYASLARQLRADLLRER"
     gene            2714..3733
                     /locus_tag="PLQ54_01495"
     CDS             2714..3733
                     /locus_tag="PLQ54_01495"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS91964.1"
                     /translation="MQRVAVVGYGFMGQMHCNAYAALPNAELAVVVDPVTEKQEKAKA
                     DHGVATVSQLESVLDDPSIALIDICLPTYMHKEATVAALAAGKHVLCEKPMALNPNEA
                     QAMIDAAERAEGFLMIAQCLRFWPEYAAAKRIVDSGELGRVKHVSCRRVSPRPTWSWE
                     NWLQQPEKSGSALLDLHVHDIDFIVYLLGKPHRVTARGVNDPTTGWDHVFATYEYDGA
                     VAFAEGAWDYPPGFAFNMSFCIGLEGGAITYNVASSPTLTVARAGTSEVEHPELPKVQ
                     ATSGATTGNISDMGGYFLEIDYLVGCIERGERPTRVPLQETALDIQIAFAEVESATRA
                     VTVAL"
     gene            3801..5105
                     /locus_tag="PLQ54_01500"
     CDS             3801..5105
                     /locus_tag="PLQ54_01500"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOS91965.1"
                     /translation="MPQLAIAGGTPVRSRPFHAWPLQTREQVEAVAQVIQSGRWSRHL
                     LRDPDGLVATGDPASPWQTDLFEREVAEFIGVSHALAVSSGTVAMDLVMRALGIGPGD
                     EVIVPAYASIGTATCVLESNAIPVFADIDPHTYTIAPKAIARCITPRTRAIVVCHVGG
                     RICDMDVIGRLGDERGLAVVEDARHAMGSRRADGRAAGTFGRASAFSFGPSSILTAGE
                     GGLIVTSDPVLDQQVRVLHDHGLELVECQPRNVAFGTSARMTELQAALLRAQLPRLEG
                     LNERRHANVKYFYEAIQLIGGLEMTTPPEPEGFRCYRDVILRYEPGAFAGAHRNRFVR
                     ALQAEGIPCSCGYDEPIYATPLFREQRFLKGSCPVNCCRVGKAPNFAHYKRTCPNAER
                     AARDEAVWFPHRLFVGPQSDVDDILYAIHKLEEHADELAAED"
     gene            5236..6465
                     /locus_tag="PLQ54_01505"
     CDS             5236..6465
                     /locus_tag="PLQ54_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91966.1"
                     /translation="MKPRPDLLDERSPRTIEVFQLSNEAGVPYSHLYMEAQVFTPDSK
                     RFILHRSATPHGGNVTDPEHHYLLCDLEDGGRLTTIIDELGCTAASVSPDGQWVYYFL
                     DRTRVNEGSLRLKRVRLDGSGRETITVIDTALPGTRYRPSLLYCLSTISSDGRRVAIS
                     AFLGDGQTPNAPFGLLVFDVQKGSADLVLEGPTWCNMHAQYSRSADHDQRRDILVQEN
                     HGNVTDARGETTVLVSGAGADIHVIRDDGTHLRDMPWGRDGNEQCQGHQCRRGRSAWA
                     ITSTDSRSPAEAQLIEGRAAPHVGHVGSETPGGVRNDLSRNFTNPHFYHFGTDIRGTR
                     LVSDAGSFDRDAQIVLMKLGEPGIEPARELTYLLSPRSSCEKGAHIHPFLSPDGRMAF
                     FNSDESGLLQAYMIRGL"
BASE COUNT         1175 a         2318 c         2084 g          919 t
ORIGIN      
        1 ccccagtccg ggtgattcag gcccacatac ctggagttcc agatctgatc ccacgtcacc
       61 tgggaccatt tcatggcctt cgcgtgcccg tcgctgaaca ggtagttgtt gacgccgttg
      121 tgccacgagg tgttgcggaa ggtatcgttg aggaaccagg ccacgtcccg gctccaaatc
      181 gacatgaacg aatcggccca cgggttggag ctgtcccaca tcaccagaaa cttcacaggc
      241 tggtagatcg actgctcgga ttcgccgtaa aggcccacat agttatagcc gtaactgatg
      301 ggcttcaggt tgttccgcca gttgttgaac atgccattgg agcgcgtcgg gcagtgatag
      361 atctccgggc ttcgctcgta cggctcgaga ggcgcgttga tgatccatcc ggggtacgtg
      421 cagttgtaac gaaaggcccg ggcgtcgttg gggctgagcg ggtcgttctg tggccaccgg
      481 cccccgctgt ccgccgtgta ttgctcgagc gccattccga tctgcttgag gttgttcagg
      541 cacgaggtct gccgggcctt ctcccgcacc cgggcgaaca cgggaaacag gatggccacg
      601 aggatggcaa taatcgccac caccacgagt agttcgacca gcgtgaaccc gcgcctcgcc
      661 atggcagaaa ccccctggtc tcgcacgacc acccgccgac aaccatgcca aaacgcgact
      721 ggaccggatc cccggccagc cgagtgccgc tccgcgtcgg cgcagagctg ttgccgtgtt
      781 cgtcgcctct gcgctgtcga cctccgtccg gcgcagtacg atgcgacatc tcattgcccg
      841 tgctttcgcg ctcggcggcg agtcacctcc ctccgaacgc acctaacgtt cgcgcaacag
      901 atccgcacgg agctggcgcg ccagcgaagc gtaggccctc ccggcttcag atgcccccag
      961 cgaggcgctc aacacggcca tttcgtcggc ggccgcgatg tgggcaagcc gcacctcacc
     1021 ccaccgttgg cgatcacccc gccccgcgcg gcgagccgct tcctcacgca gcgggaacac
     1081 gcgcagtgcc tcaaggagaa gcgagcgcgc ggcctccgga ttccgcgggg ccaccgcgca
     1141 ggcacgatcc agcagcatct cgggcgagcg tgggtccccg gtcccgctgc cgcgccgcgc
     1201 cggcgaagcc cactcggcgg ccggagacat ggccatcacg aggcccacca ccgcagcaag
     1261 tccgaggcga ggccacgagc agtgaactcg cggaccagac cgtgaccttc cctgccgcat
     1321 ccctcgaatc acgcccacgg tgaccatcac ggaggcggcg gccaagggcc acgcggcgcc
     1381 accaccgatt gccgagaccc ccagagacga gaccagggcc gcccgccatc cgcgcggcac
     1441 gtggggtccg ccatgccccc ggcaacccgc gcaggccggc acgcacaggg ccaccaccag
     1501 cagcgtggcc gggacacccg tctccaccag gcacagcagc aggtcgctcg gggccgtcgc
     1561 gggccgttca acggcctgaa acggttcggc aatgggccct ggccgtgccg gcccatagac
     1621 cgcgccaaac gaacccggcc cccaacccag accgggccgc tgggacaaca gacgccaggc
     1681 cgcgtgaccg gcgtagaccc ggagctggac ctcctcgcgg atcgaagcca ggagcggctg
     1741 gacatgcggc atccacgagt gccgcacagg cagcgccgcc acgcagacca gcaccagggc
     1801 gccgacgccg atgcccacct gcggccaccg ccagcacgcg cgccaacccg cactcgctac
     1861 tatcgccgcg ccaagcgcca gccagccgag ccatccaccg gcaagcatca aggccgccgt
     1921 actcaccgcc agctcgagcc acccgagccg gcgcgccgag gccgcacccg cggtcgccca
     1981 cgagtccacg gccagcggca gggcgaccgc aaccagagca agtgcggcag gctgaccagg
     2041 aacccacggc acatcccagg gccgctggcc gtaccccagt tcgcggtacc acgccacggc
     2101 tggccactcc ccacgagcga cagccaccaa caggcccacc gccgtcgccg accccgcggc
     2161 gcgcagcgcg gtcgcccacc cctgcagcgg aaggccttgg cgggtccatc cctcggccaa
     2221 cgccaaccac gccaagcatg ccgcgacgcc cagccatgcc gcctccggcc cggcgccact
     2281 cagggccgag gcgagtgtca gcagggcaac ggcggacagc ggaagccatg tggacatcgg
     2341 aaggcatatg agcgcaccgg aggcaaccag caggacggcg cccaacgccc ggacgcaccc
     2401 aacggatgcc gccggttgca cccactgcag gccgacacag gcggcaccgg ccagggacca
     2461 ggccatcgag cgccgcacag caggggggac acaggaggca gacatggcga cgcctggatg
     2521 atacgggcgc catcgaggag ggtcaatcaa ccgccgcgag ggttccggcg agtgaagggg
     2581 cggacgacca gatggtcgaa gtcgcgtcgg gtgagctcgc cggcagccgg gccaccagag
     2641 cacgggccgg cacgcccccg gccggcacac ccttccggct cggggacgga gtctgggcct
     2701 gggaggagac cccatgcaga gagtagcggt tgtgggatat gggttcatgg gccagatgca
     2761 ctgcaacgcc tatgcagccc tgccgaatgc cgaactagca gtggttgtcg atccggtgac
     2821 ggagaagcaa gagaaggcca aggccgacca tggcgtcgcg accgtgagcc agctggagag
     2881 cgtgctcgac gacccctcca tcgccctgat cgacatctgc ctccccacat acatgcacaa
     2941 ggaagcgacg gtagccgcat tggctgcggg gaagcacgtg ctgtgcgaga aacccatggc
     3001 cctcaacccg aatgaggccc aggccatgat cgacgcggcc gagcgcgccg agggcttcct
     3061 gatgatcgct cagtgcctgc gtttctggcc cgagtacgcg gcggcgaaac gtatcgtcga
     3121 tagcggcgaa ctcggcagag tcaaacacgt ctcgtgccgc cgcgtgagcc cacggcccac
     3181 atggtcgtgg gagaactggc tccagcaacc ggagaagtcg ggttcagccc tcctggacct
     3241 gcacgttcac gacatcgact tcatcgtgta cctgttgggc aagccgcaca gggtaaccgc
     3301 tcggggcgtg aacgacccca ccaccggctg ggaccatgtc ttcgcgacct atgaatacga
     3361 tggcgccgtc gccttcgccg aaggtgcatg ggactatccg cccgggttcg ccttcaacat
     3421 gtccttctgc atcggcctcg aaggcggggc gatcacgtac aacgtcgctt ccagtccgac
     3481 actcacggtg gcgcgggccg gtacatccga ggtcgagcac ccggagctgc ccaaggtcca
     3541 ggcgaccagc ggggccacca cgggcaacat ctccgacatg ggtgggtact tcctcgagat
     3601 cgattacctt gtgggctgca tcgaacgggg ggagcgcccg acccgggtac cgctgcagga
     3661 gaccgccctc gacatccaga ttgcgttcgc cgaagtcgag tcggcaactc gagcggtgac
     3721 cgtggccctc tagtggaagc catggctggg ccggggtgcg ccccggccca gcatccagcc
     3781 ccagggaaga gggagccgac gtgccccagc tcgccatcgc gggcggaaca cccgtgcgaa
     3841 gtcggccgtt ccatgcgtgg cccctgcaga cgcgcgaaca ggtcgaggca gtagcgcagg
     3901 tgatccagag cgggcggtgg agccgccatc tgctgcgcga ccccgatggc ctggtggcga
     3961 cgggtgaccc ggcctcaccg tggcagacgg atctgttcga gcgcgaggtc gccgagttca
     4021 tcggcgtgag ccacgcgctg gcggtctcat ccggcaccgt ggccatggat ctggtcatgc
     4081 gggccctggg gatcggcccc ggcgacgaag tcatcgtgcc cgcctatgcc tccatcggca
     4141 ccgcgacttg tgtgctcgaa agcaacgcca tccccgtgtt cgcggacatc gacccgcata
     4201 cgtacaccat cgcccccaag gcgatcgcgc gctgcatcac ccctcgcacc cgggccatcg
     4261 tggtgtgcca tgtcggggga cgcatctgcg acatggacgt catcggcagg ctgggtgacg
     4321 agcggggcct ggcggtggtc gaagacgcgc gtcacgccat gggcagccgg cgagccgacg
     4381 gtcgcgcggc gggcaccttc ggccgggcat ccgcgttcag cttcggaccg tccagcatcc
     4441 tgaccgctgg cgagggaggc ctcatcgtca cctccgaccc agtcctcgac cagcaagtcc
     4501 gcgtgctgca cgaccatggg ctcgagctgg tagagtgcca gccgcgcaat gtggccttcg
     4561 gcacatcggc ccgcatgacc gagcttcagg cggctctgct ccgggcccag ctgccgcgct
     4621 tggagggtct caacgagcgc cgccatgcga atgtgaagta cttctacgaa gccatccaac
     4681 tcattggcgg cctcgagatg accaccccgc ccgagcccga agggttccga tgctaccgcg
     4741 acgtgatcct ccgctacgag ccgggagcgt tcgccggggc ccaccgcaac cgcttcgtcc
     4801 gggcactgca ggccgaggga atcccctgct catgcgggta cgacgagccg atctacgcca
     4861 cgccactctt ccgcgagcaa cggtttctga agggctcctg cccggtgaac tgctgccgtg
     4921 tggggaaggc gcccaacttc gcgcactaca agcgaacatg cccgaacgcc gaacgggctg
     4981 cgcgcgacga ggctgtctgg ttccctcacc ggctattcgt gggaccgcag agcgacgtgg
     5041 acgacattct ctacgccatt cacaagctcg aggagcatgc cgatgagctc gccgccgagg
     5101 actagcggct ggaaccctct gaccacgcgg cgtgggtttc tccaaagggc ggccggcatg
     5161 gccggcgccg ccgctgccat cggaaccacg gcggcctggt cgcgtccgag tgcccgcacg
     5221 gggaggtcga cccgcatgaa gccgaggccc gacttgctcg acgagcggtc cccgaggacc
     5281 atcgaggtgt tccagctcag caacgaagcc ggggtgccct actcacacct ctacatggag
     5341 gcccaggttt tcacgccaga ctcgaagcgt ttcatcctcc accgctccgc cacaccgcac
     5401 gggggaaacg tcacggaccc agagcaccac tacctgctgt gcgacctgga ggacggcggc
     5461 cggctcacaa cgatcatcga cgaactgggc tgcacggccg catccgtcag cccggatggc
     5521 cagtgggtct actacttcct ggaccgcacc cgcgtgaacg aaggctccct ccgcctgaag
     5581 cgagtccgtc tcgacgggtc cgggcgggag acaatcaccg tcatcgacac ggcactgccg
     5641 ggcactcgct accgtccctc gctgctctat tgcctctcca ccatctcgtc ggacggacgc
     5701 cgcgtcgcaa tatcggcctt cctgggcgac ggtcagacgc ccaacgcccc cttcggcctg
     5761 ctggtattcg atgtacagaa gggcagcgcc gacctcgtcc tcgagggacc cacatggtgc
     5821 aacatgcacg cccagtactc ccggtcagcc gaccatgacc agcggcgcga catcctggtt
     5881 caggagaatc acggcaacgt gaccgacgcc cgcggggaga ccacggtcct ggttagcggc
     5941 gccggggccg atattcacgt catccgggat gacggcacac acctccgtga catgccctgg
     6001 ggccgcgacg ggaatgagca gtgccagggg caccagtgcc ggcgaggccg gagcgcttgg
     6061 gcgatcacga gcacggactc gcggtccccg gccgaagcac agctcatcga gggccgggcg
     6121 gctccgcacg tggggcacgt gggttctgag accccgggag gtgtgcggaa cgacctctcc
     6181 cgcaacttca ccaatccgca cttctaccac ttcggcacgg acatccgagg tacgcgcctc
     6241 gtctcggatg cgggctcctt cgaccgcgat gcgcagatcg tgctaatgaa gctgggcgag
     6301 ccgggcatcg agcccgcgcg agagctgacg taccttctga gcccccgttc gtcgtgcgag
     6361 aagggcgccc acatccaccc attcctgtca cccgatgggc ggatggcctt cttcaactcc
     6421 gatgaatcgg ggcttctgca ggcatacatg atccggggcc tgtagctgcc cgttgtggcg
     6481 ccggcgccgg accaag
//
LOCUS       DAOSPL010000045         6469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_12049_length_6469_cov_2.905675, whole genome shotgun sequence.
ACCESSION   DAOSPL010000045 DAOSPL010000000
VERSION     DAOSPL010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12049_length_6469_cov_2.905675"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3066)
                     /locus_tag="PLQ54_01510"
     CDS             complement(<1..3066)
                     /locus_tag="PLQ54_01510"
                     /inference="COORDINATES: protein motif:HMM:NF027541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HOS91967.1"
                     /translation="MGTRPDCRRRPSAVRAVAALLGAVSVPAARAAPTVAIFSDPGFP
                     YFSAPYLSGPDVQAWFARLGVTADLLSAGDLADPRVFRIGRYGVLIHLYGNTFPEAAA
                     GNLRRFRQYGGGIVSAGVPFCHPCRQTGAAGWQWVASAGDAAEVTAEAAHSGSCGAKL
                     AKGGPGWSGLVQQTRIPAVAGETFTLGGWAKTRDTSGGPQADALLLRSFDAGGNFLGQ
                     AGPDLPPPGGDWTFVSAQVTTPPGTAEVDTIVALWKPPATVWLDDLALVRGELTPAAT
                     NLLPNASFEQLGGEWKDLGHTPYFGHDQMGTGGFYGSGDMGTDLVYHAGADPMKLAVL
                     GWSRWGQVYPSGATARTQALDPRTLPPGDEVTPIVEVSDAAGAWPAVAVVRHRCAEFP
                     GAIDVWAGVSLFSYGGGYLQEQLAQVELYGRGALYILHEKGALPGYAALLRRADAAYR
                     ASRPGRLSAPVGTRRSRDGVFPKSAAPARHIVAADVSRLPLDQQLALVSLEGVINGRQ
                     PRLYLITDRPGMVAKPTPQERWLEWLRERGDVAEVERVADPWSLTARWPNEIRGMIIT
                     DPDLPASVNVATMMCGLEGAIMASPTLAQRLKLPVVADLRGRWKTNVAAYEWAIADLW
                     PKLNHDFVAMMWPGWVFPRDYSIAHQGFCFWITGVRDAVPGVGSPLQEAALMSRLLAK
                     APVNIGTIGAPWAGDLVGIQEAPGVGLLSEYGKFMVWSAETGNLSVHSGTRRRALRHE
                     VPPPPALDRSKVYLSFMVSDGDAPINWCSFFLTNYWDDPARGTFPLAWSFGPTGLDLM
                     PDVMDYYYRKAGPNDAFVCACSGVGYCYPEAYASRYDHPDRVFDGFLDLTRQYMERTD
                     ERGLWTHSAGEDTLRRYAAALPAAEYFLPDYGLQPDTTVANANSLVGGVPAFHAVCGF
                     DPKGGPERARELLLADIRRFTPTTRPAFLNAFVQCYPVSPTLLKQVLDELGPEYVPVL
                     PEHLAALYRQAQTPWASPQAPASTRPKTGDRPEWSALKPNARGVCRITSV"
     gene            3285..5144
                     /locus_tag="PLQ54_01515"
     CDS             3285..5144
                     /locus_tag="PLQ54_01515"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOS91968.1"
                     /translation="MILMRAVTVAALGCVAVLAGSVLAQGTTPEGGPTPLVCVTEPGG
                     YYGDPAEVIRRRLDCYRQLGVEMLRVEPGWENGALPAELMRTDLRVKLILYVLGIPPG
                     YGAEHAGEAMVDEQGQTDWHLGPWHRELPEIVTRSAEQQMERLKALGVLDRIDEVVAD
                     LGPAGEGIYPANWTLGRDGEEAFWCYSDAAQADFRAAMQAKYDSVAAADDAWRLTGAT
                     RFAAWNEVRIPQPGTPWARGPFWEDMLTWYREAKRRMMEFLIDNTVRIARRYLGDRAK
                     AIIYLPGTAFGEEEWDQAVATAGGSGSVRLMVDTDWLIDQALAKGCLLQYTGAENALE
                     VARIVAKLKARGVPDCSAMWAENAGVENAGRDPMWLAQIVCAYSLRGVDLTWSSWLFE
                     ADHTTPSPTYAEFARAAAALRHYRGTGEPPDPSDCGIGSVRQLEPGRYELTPNADTRL
                     MGSFPDTIKGWDPEMAVVEGGQTQRTLIRFPLDRLPAGRALRSATLTLHSFIDYGDAS
                     TVAVNVYRVTTPWQEHAATWRARTLTADWEQPGGDAAGPYATTIVGPVKPGEAIEWNI
                     SALCREWLAGKAPNYGLMVSLASTEPANKSIAAKEHPNPAMRPRLVIELGEGE"
     gene            6002..>6469
                     /locus_tag="PLQ54_01520"
     CDS             6002..>6469
                     /locus_tag="PLQ54_01520"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOS91969.1"
                     /translation="MLNVLWLAAVSPQVTLSEEPVPYAVAAEPWEQGLGNHRARVRVA
                     ADAPAVWAHIPWRRRDRDPERKRIIVMAPSGQRVNDAVALRINRIAGDIAFRPTEGPG
                     EYCVYYLPFVVQDAYGWYGGDYLAPDDTADAQWRTDNGLAADQLADDAWRSLPR"
BASE COUNT         1023 a         2284 c         2214 g          948 t
ORIGIN      
        1 tacagaggtg atgcgacata cgcctctggc attgggctta agtgcggacc attcgggacg
       61 gtcaccggtt ttcggccgcg tgctggccgg ggcttgcgga ctcgcccaag gggtctgcgc
      121 ctgccggtag agcgccgcca ggtgctccgg gagcaccggc acgtactccg gccccagttc
      181 atccaacacc tgcttcagca gcgtcggcga gaccgggtag cactgcacga aggcgttgag
      241 gaatgcgggt cgcgtcgtgg gcgtgaaccg gcggatgtcg gccaggagca gctccctggc
      301 tcgctccggg ccgcccttcg gatcgaagcc gcacaccgcg tggaaggccg gcacaccgcc
      361 gaccaagctg ttcgcattgg ccaccgtcgt gtcgggctgc aggccgtagt ccggcaggaa
      421 gtactcggct gccggcagcg ccgcggcgta ccgcctcagc gtgtcctcgc cggccgaatg
      481 ggtccacagc ccgcgctcgt ccgtccgctc catgtactgg cgggtcaggt cgaggaagcc
      541 gtcgaacacg cggtcgggat ggtcatagcg gctcgcgtac gcctcggggt agcagtaccc
      601 gactccggag cacgcgcaca cgaaggcgtc attcggcccg gccttgcggt agtagtagtc
      661 catcacgtct ggcatgaggt cgagcccagt tggcccgaag ctccaggcca gcgggaaggt
      721 gccgcgcgcc gggtcgtccc agtagttcgt caggaagaag ctgcaccagt tgatcggcgc
      781 gtcgccgtcc gagaccatga agctcaagta cactttggat cggtcgagcg ccggtggcgg
      841 cggcacctcg tggcgaagcg ctcgccgcct cgtgcccgag tggacgctca ggttgcctgt
      901 ctcggccgac cagaccataa acttgccgta ctcagagagc agcccgacac cgggggcctc
      961 ctggatgccc accagatcgc ccgcccacgg cgccccgatc gtgccgatgt tcacgggcgc
     1021 cttcgccagc agcctcgaca tcagcgcagc ctcctgcagg ggcgaaccca ctcccggcac
     1081 cgcgtctctc actccggtga tccagaagca gaagccctgg tgcgcgatgc tgtagtcgcg
     1141 cgggaacacc catccgggcc acatcatcgc cacaaagtcg tggttcagct tcggccacag
     1201 atcggcgatg gcccactcgt atgccgccac gttggtcttc catcggcccc gcaggtcagc
     1261 aaccacgggg agcttgagtc gctgggccaa cgtcggcgag gccatgatcg ccccttcgag
     1321 cccgcacatc atcgtcgcca cgttgacgga cgcgggcagg tcggggtcgg tgatgatcat
     1381 gccccggatc tcgttcggcc accgggcggt cagcgaccac ggatcggcga cgcgctcgac
     1441 ttccgcaacg tccccgcgct ccctgagcca ttcgagccag cgctcctggg gcgtcggctt
     1501 cgccaccatc ccggggcggt cggtgatgag gtacagccga ggctgcctgc cgttgatcac
     1561 cccctcgagc gacaccagcg cgagctgctg atccagcggc agccggctca catcggccgc
     1621 cacgatgtgc cgcgcgggcg cggcgctctt cgggaacaca ccatcgcggc tccgccgcgt
     1681 cccgacaggc gccgacagac gccccggcct cgacgccctg tacgccgcat ccgcgcgccg
     1741 caggagggcc gcgtaccccg gtagggcgcc cttctcgtgc aggatgtaca gtgcgccccg
     1801 gccgtacaac tccacctgcg cgagctgctc ctggaggtag ccccctccat aggagaagag
     1861 cgacacgccg gcccacacat cgatcgcgcc ggggaactcg gcgcaccggt gtcgcaccac
     1921 ggcgacggcc ggccaggcac cggccgcatc gctgacttcc acgatcggcg tcacctcgtc
     1981 gcccggcggc agcgtcctcg ggtcgagtgc ctgcgtacgc gccgtggcac cggacgggta
     2041 cacctgcccc caccgcgacc agcccagcac agccagcttc atcggatcgg ccccggcgtg
     2101 gtagaccaga tccgtgccca tgtcacccga tccgtagaac ccgccggtgc ccatctggtc
     2161 gtgaccgaag tagggggtgt gccccaggtc cttccattcg cctccgagct gctcgaagct
     2221 cgcgttcggt agcaggttcg tcgcggcggg cgtcagctcc ccacgcacca gcgccaagtc
     2281 gtcgagccac accgtcgccg gcggtttcca gagagcgacg atggtgtcca cctccgccgt
     2341 ccctggcggc gtggtcacct gagccgagac gaaagtccag tcgccgccgg gcgggggcag
     2401 atccggcccg gcctgcccca ggaagttacc accggcatcg aaggaccgca ggagcagcgc
     2461 gtcggcctgt ggcccgccgc tggtgtcgcg cgtcttggcc cagccgccga gggtgaatgt
     2521 ttcgccagcg accgctggga tgcgcgtttg ctgcacgagc ccggaccatc ccggcccgcc
     2581 cttggcgagc ttggcgccgc acgacccgct gtgggccgcc tcggcagtaa cctcggccgc
     2641 atcccctgcg cttgccaccc actgccaccc agctgcccca gtctgccggc acgggtggca
     2701 gaagggcacg ccggccgaca caatgccgcc gccgtactgg cggaagcggc gcaggttccc
     2761 ggccgccgcc tcagggaagg tgttgccata gaggtgaatg agcaccccgt atcggccaat
     2821 acggaacact cgcggatcag ccaggtcgcc agcgctgagc agatcggccg tcacgcccag
     2881 ccgtgcgaac catgcctgca catcgggccc cgacaggtag ggtgcggaga agtaggggaa
     2941 gccggggtcg ctgaagatcg ccaccgtcgg cgcggcccgg gccgctggga ccgagaccgc
     3001 gccaagcaac gccgccaccg cccgtaccgc cgacggtctg cggcgacagt caggtcgcgt
     3061 ccccatggcc gctccccccc tccgtcatcc gggccttggg catcgtcgca cactctccct
     3121 ggttcggacc gatgccccac ggcgcggcgt cttccatcgt cggctcgcag cgacctctcc
     3181 ggccgcaggc aagtggcagc ctggcccgtg aggaagctag acgcggagcc gacacccaca
     3241 ggcggcgttg gagccaaggc ggagcgaatg gcgatggggc aaccatgatc ctcatgcggg
     3301 cagtgaccgt ggccgcgctc gggtgcgtcg ccgtgctggc cggcagcgtc ctggcgcaag
     3361 gcacgacccc ggagggcggg ccgacgccgc tcgtgtgcgt gacggagccc ggcggctact
     3421 acggagaccc ggcagaggtc atccggcggc gactcgactg ctaccggcag ctcggcgtcg
     3481 agatgctgcg ggtggagccc ggctgggaga acggggcgct gccggcagag ctgatgcgca
     3541 ccgatctgcg ggtgaagctg atcctctatg ttctggggat cccacccggg tacggcgccg
     3601 aacatgccgg cgaagccatg gtggacgagc agggccagac cgactggcac ctggggccgt
     3661 ggcaccgcga gctgcccgag atcgtgaccc ggagcgccga gcagcagatg gagcgtctga
     3721 aggccctggg cgtgctggac cgcatcgacg aagtggtcgc cgatctcggc cctgcgggcg
     3781 agggcatcta tcccgccaac tggaccctcg gccgggacgg cgaggaggcc ttctggtgct
     3841 actccgacgc ggcgcaggcc gacttccgtg cggccatgca ggccaagtac gactcagtag
     3901 cggccgccga tgacgcgtgg cggctcactg gcgcgacgcg cttcgccgcc tggaatgagg
     3961 tgcggatccc gcagccaggg acgccctggg cgcgcgggcc gttctgggag gacatgctca
     4021 cgtggtaccg tgaggcaaag cgcaggatga tggagttcct gatcgacaac accgtccgca
     4081 ttgctcgcag gtacctgggc gaccgcgcca aggcgatcat ctacctcccg ggcacggcgt
     4141 tcggggagga ggagtgggac caggcggtcg cgacagctgg cggctcgggg tccgttcggc
     4201 tgatggtcga cacagactgg ctcatcgacc aggccctcgc gaaggggtgc ctgctgcagt
     4261 acaccggggc agagaacgcg ctcgaagtgg cgcgcatcgt cgccaagctg aaggctcgcg
     4321 gcgtccccga ctgctcggca atgtgggcag agaacgcggg cgtcgagaac gccgggcgcg
     4381 accccatgtg gctggctcag atcgtgtgcg cctacagcct tcggggtgtc gacctcacct
     4441 ggtcgagttg gctgttcgag gcggaccaca ccacgccctc gccaacctat gccgagttcg
     4501 cgcgagcggc ggcggccctc cgccactatc gcggcacggg cgagcccccc gacccctctg
     4561 attgcggcat cggctctgtg cgccagctgg agccggggcg gtacgagctc acccccaacg
     4621 ccgacacgcg gctgatgggg agcttccccg acacgatcaa gggttgggac ccggagatgg
     4681 cggtggtcga gggcgggcag acacagcgga cgctgatccg atttcctctg gaccgcctgc
     4741 cggctggtcg ggctctcaga tcggcgacgc tgacgctcca cagtttcatc gactacggcg
     4801 atgcctcgac ggtagcggtg aacgtgtacc gcgtcaccac cccatggcag gagcacgcgg
     4861 caacgtggcg ggcgcggacg cttaccgcag actgggagca gccgggcggc gatgcagcag
     4921 ggccgtacgc gacgacgatc gtcggccccg tgaagccggg cgaggccatc gagtggaaca
     4981 tcagcgccct atgccgcgag tggctcgcgg ggaaagcgcc gaactacggc ctgatggtct
     5041 cgctggcttc gacggagcca gccaacaagt cgatcgcggc caaggagcac ccaaacccgg
     5101 ccatgcgtcc gcggctggtg atcgagctgg gcgaggggga gtagggtggg cgcgtgggcg
     5161 ggccgtctca gtcggctcag gggatgcgct gggtgggacg ctcgatctac gcgcaccggc
     5221 agaaccagac gcctccctcg cgggccggca gcgacaccgt taggccctcg gcctcgagcg
     5281 cctcgccggt catcagccgc tcgttacccg tctccggccg gtcggactgc cacgcgaccc
     5341 actgctcccc gctgcggtcg aacagcggca gcgggagtag tccccatagg tcagcgggcc
     5401 agacgcgcga cgccccggca cgatcatctg ccggcccagc gcggactcgt agtcatcgca
     5461 tcgacctcct cgtcagagag cccctcaagg cactgcggtg tcctgatgca tactctgtcc
     5521 ggggctcagt cgcaaacatt cgtaatcgct ttatattatg caaggcgcca ccaggcatgg
     5581 ctatataggt aatgaccgtt ttgactatct cccatgcgag cttcagggcg ttccacgcgc
     5641 tgcgggttcg cggtgctgcc ggccgaggcc ccgctccgcc gccgcaagag catcgggccg
     5701 tatgcgctga gaccggccgc gcattgcgcc ggcgtcgtgc cgtcggagtc ggtctcgtgg
     5761 agcagtcgct gcccgtccca gaggaaccag agcccaccca gccccggggt ccttcgccgc
     5821 gcgtggaggg acttcatgga cggggcgccg cgagggcggt gtggcagcgg aacgggcccc
     5881 gggaagacag ccttctcgtg caggtccgcg gcaatgcacc gaaggtgttc gccgcacggc
     5941 ggcgtaacgt tgtagttcag cgccgcacgc gatgcgcggc tcagcgccgg gaggtctcac
     6001 catgctcaat gtgctctggc tggcggcggt cagcccccaa gtcacgcttt ccgaggagcc
     6061 cgtgccctat gcggtggcgg ccgagccctg ggagcagggg ctcggcaacc accgggcccg
     6121 ggtccgagtc gcggccgacg cgccggctgt ctgggcgcac atcccctggc ggcggcgcga
     6181 tcgcgacccc gaacgcaaac gcatcatcgt catggccccc agcggccagc gggtcaacga
     6241 tgccgtagcc ctccgcatca accgcatcgc cggcgatatc gcgttccgtc ccaccgaagg
     6301 cccgggcgag tactgcgtct actacctccc gttcgtcgtc caggacgcct atggctggta
     6361 tggcggcgat taccttgccc ccgatgacac agccgatgcc cagtggcgaa ccgacaacgg
     6421 cctggccgcc gatcaactcg ccgacgatgc ctggcgctcg ctaccgagg
//
LOCUS       DAOSPL010000046         6451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_12100_length_6451_cov_3.061288, whole genome shotgun sequence.
ACCESSION   DAOSPL010000046 DAOSPL010000000
VERSION     DAOSPL010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12100_length_6451_cov_3.061288"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="PLQ54_01525"
     CDS             <1..1118
                     /locus_tag="PLQ54_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS91970.1"
                     /translation="ADTLLLERYGCLGGLATGGLVLVLPSFEDSGRPIIGGIGLETRT
                     RLVEAGASVCRGGADAAFFDPEALKWLSQHMVRESGARILHHVWIASAHVEGQRVRAA
                     IVESKAGRFAVAGRVFIDCTGDGDLFAAAGASFEASTQQIGLPFRLCHVDVARWKQAA
                     ADDRAAVGEAIQAAARRAGFEGWLGLEAMPTPDGLVWCNNMLCDRDGLSPEDLSYVET
                     AGRDAIRRIVTELRATVPGFERAWLVDTASQVGVRRSRRLIGEYVMGDEDTAQFDRRF
                     DDAVGRGNDFRQQGIAFDFPYRALVPREVDGLLVAGRCVSCSHEALEPIREIHNCWVM
                     GEAAGTAAALSLRLGCPVRDVPIASLQADLRGAGVVL"
     gene            complement(1132..2790)
                     /locus_tag="PLQ54_01530"
     CDS             complement(1132..2790)
                     /locus_tag="PLQ54_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOS91971.1"
                     /translation="MSLPIGSTKSRTWPKGMWMVSVISVALLGAAWQVGCRARAADAA
                     TEEGPAAAEAVTPDAPELRTAEIWMGAYMGESKIGYQCISMFPDSVDAANKVDPLVLG
                     KDAGTTTLKVIPDWYDGEQVQVVRATGIQRIQMMGRPVEQTTELNEYRTPDGQMLAMV
                     FRMSSAGMSQRMIATFDKDKIRYVQSTEQDEKTGEVDIPDGADFRDPEYGFKVQDPQP
                     GQTLVYHQFMPLLLRVVKFEATVVGPEEIEMDGKKVRAIRYEHKSDIISQTSWVDEQG
                     DPLKTVAAIMDLRFVREAPEVAKTMEGPGGYVPSPDLVAKLQLTVEKPIEKPREVRRM
                     QARISRIARESLILSDERQTVTEVTKEADGTYSALYEVRSLDPPATALPLPIDEPAMA
                     DYLKPTTHIQCTDPRLVNQARQIVGDEKDSLKAAQAIARWVSMTMRTRADIGLVRSAL
                     EVLQSKEGVCRDYAALYAGLARAAGLPTRFVSGIVYWKSGFFYHAWDETYVGEWVAFD
                     STVAPGYPVDATHIKFSEGDVADMVEMVPIIGALKLDIRDVAPM"
     gene            complement(2982..4004)
                     /locus_tag="PLQ54_01535"
     CDS             complement(2982..4004)
                     /locus_tag="PLQ54_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS91972.1"
                     /translation="MASRPKKIRVGFIGAGGISRGHFSRLKTHPKAEVVALCDTSEKS
                     IEAMHAHDPDSQACAVFSDWKTMLHEVELDAVQIHTPHTQHFEQAMASLDRGLHVLCE
                     KPMVCKVAHAEKLMRKIEASGKVFGISYQRHHQPEFLYIRKCLLEGKYGRVQYITALQ
                     SQDWYRWCTRPGVWRGIPELSGGGQLNDSGSHLLDIILWCSGLVPAEVFAYIDNLDSQ
                     VDILTAASVKFTNGALGTLSVNGNGIGFYEDITFYCDEGAFYMRQGKLTEQNREKGYF
                     EPTKMPKGTDPDTNWIDAILGKAEMIVPAVCGLRVIQLTEAAWNSGKSGRPSKVKLSE
                     LDTPAA"
     gene            4219..5316
                     /locus_tag="PLQ54_01540"
     CDS             4219..5316
                     /locus_tag="PLQ54_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013917263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS91973.1"
                     /translation="MERVRVGVVGCGAICGAYLGAGQQFRVLDFVACADLLPERAQAR
                     AQEFGVPRVCSVDDLVADPNVDVVLNLTVPKAHAGVATAALRAGKHTYAEKPFAVSRE
                     EGRPVYELAKKKQLRIGCAPDTFLGAGIQTCRKLIDDGWIGRPIGAAANMICHGHEDW
                     HADPEFYYTTGGGPLFDMGPYYLTALVNLLGPVKRVTGSATITYPQRVVTSQPKYGTV
                     LQVECPTHVMGIMEFANGAIGTIAMSFDVWAAQVPCLEIYGTEGTLAAPDPNCFGGPV
                     RLWRNGAWSEVPLSHGYAEQSRSIGLADMAYGIRTGRPHRASGEMAFHVLDVMCGFYD
                     AWEQGKWVALETTCERPAPLPLGLPAGTLDE"
     gene            complement(5350..>6451)
                     /locus_tag="PLQ54_01545"
     CDS             complement(5350..>6451)
                     /locus_tag="PLQ54_01545"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOS91974.1"
                     /translation="SSPGWQPPAAGPGGLSGVVQRCMAPRVEDRPADGQAVLDMVKAQ
                     VPPPKPRGRPRPITGLVAPQNPSSSRARERKPGVWQRVRRLGTSPARAAVRGTTAVSL
                     APPVSMTAGLIGALLLPLLIGLPMLALYVRWQRSVPDEVTVPKVVGKTVEQARRLLLD
                     RELKLAVVDETYDPSIPAGQIVWCKPPAGRKVKEGRTISVRVSRGARQVTVPEVTRLK
                     ASEARRRLQEAGLHAADGRMAYSDIVSEGAVISQSPQAGRKLLEGSEVVLLVSKGPRA
                     GEQARSPERTRKVDPVIAKLERRTAKVTVDVPQAATAQRVQIRVVDAEGERVVYDGLH
                     EPGDHIEETVAGRGPATVSVILDDQVIHQQAL"
BASE COUNT          976 a         2178 c         2142 g         1155 t
ORIGIN      
        1 gagccgacac gctgctgctt gagcgctatg gctgcctcgg tggcctggcc accggcggcc
       61 tggtgctcgt gctgcccagc ttcgaggact ccggccggcc catcattggt ggtatcgggc
      121 tcgagacccg cacgcgcttg gtcgaggccg gcgcgtccgt ctgccggggc ggggccgatg
      181 cggccttctt cgaccccgag gcgctgaagt ggctctcgca gcacatggtg cgcgaatctg
      241 gcgcgcggat cctccatcac gtctggatcg cgtcggcgca cgtcgagggg cagcgtgtgc
      301 gggccgccat cgtcgagagc aaggctggtc gcttcgccgt ggcggggcgg gtgttcatcg
      361 actgcacggg cgatggtgat ctcttcgccg ctgccggagc atccttcgag gcgagcaccc
      421 agcagattgg cctgcccttc cggctgtgcc atgtggacgt cgcccggtgg aagcaggctg
      481 cagctgacga tcgtgcggcg gttggtgagg ccatccaggc ggccgcgcga cgcgcgggct
      541 tcgaaggctg gctggggctc gaagccatgc ccacgcctga cggcctggtg tggtgcaaca
      601 acatgctgtg cgatcgcgat ggtctctcgc ccgaggacct gagctatgtg gagacggcgg
      661 gccgtgatgc gatccggcgc attgtgaccg agctccgcgc cacggtgccg ggcttcgagc
      721 gtgcctggct ggtcgatacg gcctcgcagg ttggcgtgcg ccgctccagg aggctgatcg
      781 gggagtacgt catgggcgat gaggacacgg cccagtttga ccggcggttc gacgacgctg
      841 tcggccgggg caacgacttc cgccagcagg gcatcgcctt cgacttcccc taccgggcac
      901 tcgtcccgcg ggaggtcgat ggactcctgg tcgccgggcg gtgcgtctcg tgcagccacg
      961 aggcgctgga gcccatccga gagatccaca actgctgggt gatgggcgaa gccgccggca
     1021 ctgcggccgc cctctctctg cgcctcggat gccccgtacg cgacgttccc attgccagcc
     1081 tgcaggccga cctccgcggc gccggcgtgg ttctgtagcg aagcgggggt gctacatcgg
     1141 cgcgacgtcc cggatatcga gcttcagcgc cccgatgatg ggcaccatct cgaccatgtc
     1201 ggccacgtcg ccctcagaga acttgatgtg cgtggcatcc accgggtagc ctggcgccac
     1261 cgtgctgtcg aacgctaccc actcgcccac gtaggtctca tcccacgcat ggtagaagaa
     1321 ccccgacttc cagtagacga taccggagac gaaccgcgtc ggcagcccgg cggcgcgcgc
     1381 gaggccggcg tacagggccg catagtcccg gcacacgcct tctttggact gcaggacctc
     1441 gagcgctgaa cgcaccaggc cgatgtcggc ccgggttcgc atggtcatgc tcacccaccg
     1501 cgcgatggcc tgggcggcct tcagcgagtc cttctcgtcg cccacgatct gtcgggcttg
     1561 gttgaccagg cgggggtcgg tacactggat gtgcgtcgtg ggcttgaggt agtcggccat
     1621 ggccggctcg tcgatgggca acggcagcgc ggtcgccggc gggtccaggg accggacctc
     1681 gtagagggcg ctgtaggtcc cgtctgcttc cttggtcacc tcggtgaccg tctggcgctc
     1741 gtccgacaga atgagactct cgcgggcgat acggctgatc cgggcctgca tcctccgtac
     1801 ttcgcgcggc ttctcaatgg gcttttcgac cgtgagctgc agcttggcca cgaggtcagg
     1861 cgaggggacg tagccgccgg gtccctccat ggtcttggcg acctcgggcg cctcgcgcac
     1921 gaaccggagg tccatgatgg cggcaaccgt cttgaggggg tcgccttgct cgtcgaccca
     1981 gctggtctgg ctgatgatgt cactcttgtg ttcgtatcgg atggcgcgaa ccttcttgcc
     2041 atccatctcg atctcttcgg ggcctacgac tgtcgcctcg aacttcacga cgcgcaggag
     2101 taggggcatg aactggtgat agacaagagt ctggccaggc tgagggtcct gaactttgaa
     2161 gccgtattca ggatcgcgga agtcggcgcc gtccgggatg tccacctctc cggtcttctc
     2221 gtcctgctcg gtcgactgca cgtaccggat cttgtctttg tcgaacgtcg cgatcatccg
     2281 ctggctcatg cccgccgacg acatgcggaa caccatggcc agcatctgac catcgggggt
     2341 ccggtactcg ttgagttcgg tggtctgttc gaccgggcgc cccatcatct ggatccgttg
     2401 aatgcccgtg gcgcggacga cctggacttg ctcgccgtca taccaatcgg ggatcacctt
     2461 cagtgtcgtc gtgccggcgt cctttccgag caccagcggg tcgaccttgt tggcggcatc
     2521 gaccgaatcc ggaaacatgg agatgcactg gtagccgatc ttggactcgc ccatgtaggc
     2581 gcccatccag atctcggccg tcctcagttc gggtgcatcg ggcgtcaccg cttcggctgc
     2641 cgcgggtccc tcctcggtcg cggcatcggc cgcacgggcc ctgcagccta cctgccatgc
     2701 ggcgccgagt agcgcgacgc tgatcacgct caccatccac atccccttgg gccaggtcct
     2761 tgacttcgtt gacccgatcg gcagagacat tcgcgatacc tccacagcca ccagacccca
     2821 caggggaggc cgtcagatga cctccatcat accgaccgca gtggcaacgg tcacccaccg
     2881 ccgcctctcg ccgcctgcgc acgccaggtc acgggtacag accgagatcc ccggcgcccc
     2941 gcaagagccc tggcctgtgg cccaggttcc ccaggctggc gttatgctgc cggcgtgtcc
     3001 agctccgaca gtttcacctt cgaaggccgc ccgctcttgc ccgagttcca cgcagcctcg
     3061 gtgagctgga tgacccggag gccgcagaca gccgggacga tcatttccgc tttgcccaag
     3121 atcgcgtcga tccagttggt gtccgggtcg gtgcccttgg gcatcttcgt cggctcgaaa
     3181 tagcccttct cgcggttctg ctccgtgagc ttgccctgcc gcatgtagaa agcgccctcg
     3241 tcgcagtaga acgtgatgtc ctcgtagaag ccaatgccat tgccgttcac cgacagcgtc
     3301 ccgagggcac cattcgtgaa cttgacgctg gccgcggtga ggatgtcgac ctgcgagtcc
     3361 aggttgtcga tgtacgcgaa cacctcagcc gggaccagcc ccgagcacca caggatgata
     3421 tccagcagat gacttcccga gtcgttgagc tgaccgccac ccgagagctc cgggatgccg
     3481 cgccacacac cgggccgcgt gcaccagcgg taccagtcct gcgactgcag cgcggtgatg
     3541 tactgcaccc ggccgtactt gccctcgagc aggcacttgc ggatgtagag gaactcgggc
     3601 tggtggtgcc tctggtacga gatcccgaag accttgcccg atgcctcgat cttgcgcatg
     3661 agcttctcgg catgggccac tttgcacacc atgggtttct cgcacagcac atgcaggccg
     3721 cggtccagcg acgccatggc ttgctcgaag tgctgtgtgt ggggagtgtg gatctggacc
     3781 gcatcgagct cgacctcgtg gagcatggtc ttccagtcgg agaacacggc gcacgcctgc
     3841 gagtcgggat cgtgtgcatg catcgcctca atgctcttct cgctggtgtc acacagggcg
     3901 acgacctcgg ctttggggtg tgtcttgagc cgggagaagt gcccgcggga gatcccgccg
     3961 gcgccgatga agcctactct gatcttcttg ggtctgctgg ccatgcgagg gtctcgctcc
     4021 caccgaagcc cggggcacct cggtcccggg ccgcgctccg ccccgtaagc gggacacggc
     4081 gcccatgtgg cgtcggcgca gtattgctcg ctgggggaag caggacctcc catcgcggtg
     4141 cctctgcggc aggtggcctt ggctgcgcac ccgaaccagc gagcatcttc gtaccatggg
     4201 ggagtcgatc caagagccat ggagagagtt cgagtcggtg tggtcggttg cggtgccatc
     4261 tgcggggcct acctgggagc cgggcagcag ttccgcgtcc tggacttcgt cgcgtgtgcg
     4321 gacctgttgc ccgagcgggc ccaggccaga gcgcaggagt tcggcgtgcc gcgcgtctgc
     4381 tccgtcgacg acctggttgc tgacccgaac gtcgacgtcg tgctgaacct gacggtccca
     4441 aaggcgcacg ctggtgtggc aacggccgct ctgcgggccg gcaagcacac ctacgccgag
     4501 aagcccttcg ccgtcagccg cgaggaaggc cgtccggtct acgaactggc aaagaagaag
     4561 cagctccgga tcggctgtgc gcccgacacc ttcctcggtg caggcatcca aacctgccgc
     4621 aagctgatcg acgatggctg gatcggaagg cccatcggtg ccgccgccaa catgatctgc
     4681 cacggtcacg aggactggca cgcggatcct gagttctact acacgacggg cggcggcccg
     4741 ctgttcgaca tggggcccta ttacctgacg gccctcgtga acctgctggg gcccgtgaag
     4801 cgcgtcaccg gatcggccac gatcacctat ccccagcgcg tcgtcaccag ccagcccaag
     4861 tacggcacgg tgctccaagt cgagtgcccc acgcatgtca tgggcatcat ggagttcgcg
     4921 aacggcgcca tcgggacgat cgccatgagc tttgacgtct gggcggctca ggtgccgtgc
     4981 cttgagatct acggcaccga gggcacgcta gccgcaccgg atcccaactg cttcggcgga
     5041 ccagtgcgcc tctggcggaa cggtgcgtgg agcgaggtgc cgctgagtca cggctatgct
     5101 gagcagagcc gcagcatcgg attggctgac atggcctacg ggattcgcac gggacggccg
     5161 caccgagcga gtggcgagat ggccttccac gttctcgatg tcatgtgcgg cttctacgac
     5221 gcatgggagc aaggcaagtg ggttgcgctg gagacgacct gcgaacggcc ggcgcccctc
     5281 cctctgggcc tcccggccgg cacgctggat gagtaggcga cgcgccgggg ccgctcccgg
     5341 ccggccatgc tacagtgcct gctggtggat gacctgatcg tccaggatca cggagacggt
     5401 ggctgggcct cgacccgcca ctgtctcctc gatgtggtcg ccgggctcgt ggagcccgtc
     5461 atagaccacc cgttcgccct cggcgtccac cacacgaatc tgcacgcgct gggccgtggc
     5521 ggcctggggc acatccaccg tcaccttggc ggtccggcgc tccagtttgg cgatgacggg
     5581 gtcgactttg cgggtccgtt ccggcgaacg ggcttgttct ccggcacggg ggcccttgct
     5641 gaccagcagg accacctcgc taccttccag caacttgcgg cccgcctgcg ggctctggct
     5701 gatgaccgcg ccctcgctga cgatatcgct gtacgccatt cggccatcgg cggcatggag
     5761 acctgcctcc tgaagccggc gccgggcctc actcgccttc aggcgtgtca cctccggcac
     5821 cgtcacctgc cgggcgccgc gcgagacgcg cacgctgatc gtccggccct ccttgacctt
     5881 tcggcctgcc ggcggcttgc accaaacaat ctggccggcg ggaatgctcg ggtcgtaggt
     5941 ctcatccacc acggcgagct tcagctcgcg gtccaagagc aagcggcgcg cctgctcgac
     6001 cgtcttgccc accactttcg ggacagtcac ctcgtcgggg acgctacgtt gccaccgcac
     6061 gtacagcgcg agcatgggca ggccgatcag cagcggcagc agcagggcgc caatgagccc
     6121 ggcggtcata ctcaccggcg gagccaggct gacggcagtc gtgccgcgga ccgcagcgcg
     6181 ggcggggctg gtgccgagac gcctaacccg ctgccagaca cccggcttcc gttctcgagc
     6241 gcgtgaagag gacgggttct gcggcgcgac caatcccgtg atggggcgcg gcctccccct
     6301 cggcttgggc ggcggcacct gagccttcac catatccagg accgcctggc catcggcggg
     6361 tcggtcctcg actcgtgggg ccatgcaccg ctggacgacg cccgagaggc cccccgggcc
     6421 ggccgctggc ggctgccagc ccggtgacga t
//
LOCUS       DAOSPL010000047         6449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_12106_length_6449_cov_2.959806, whole genome shotgun sequence.
ACCESSION   DAOSPL010000047 DAOSPL010000000
VERSION     DAOSPL010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12106_length_6449_cov_2.959806"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..803)
                     /locus_tag="PLQ54_01550"
     CDS             complement(24..803)
                     /locus_tag="PLQ54_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOS91975.1"
                     /translation="MANPRIAAQLIVYGGRPEFDLAGVLTELAQAGYAGAEIGTNFMD
                     ADRVQVKAAFANAGIALSGIHGGYGSIPDASWQAKAIEFCKDMGAKHIICSGVADNDS
                     LEGYIETGAVFNAFGARCKEAGLNFLYHNHAWEFRVFDGVKGIHKLCEVTDPDLVKLN
                     VDVYWVTVGGEKPEEFIERYGNRVGYYHFKDGPYTPTGSIAPGPYEFTELGRGSVDLA
                     AALAAARRHDPQWIVYEQDQSKLDPTEAARISLAHLRSLGL"
     gene            complement(990..1442)
                     /gene="nusB"
                     /locus_tag="PLQ54_01555"
     CDS             complement(990..1442)
                     /gene="nusB"
                     /locus_tag="PLQ54_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019714929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOS91976.1"
                     /translation="MQSRRQARRQALRLLYQLDVVFGFSREAVESALVRWQRYSDERP
                     ADEAVLYVADVVHGVAERVQELDALIAYYATDWSVDRMASIDRCVLRLALYEMRYRDD
                     IPLNVSINEAVELAKRYGDSASGTFVNGVLAQFKRERDAHALRHQTAE"
     gene            complement(1504..2403)
                     /gene="zupT"
                     /locus_tag="PLQ54_01560"
     CDS             complement(1504..2403)
                     /gene="zupT"
                     /locus_tag="PLQ54_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOS91977.1"
                     /translation="MSGVLYALGLTLFAGMATGIGSMIAFAAKPTNFRFLSVATGFSA
                     GVMLYVSFVEIFHKGTVALVEAYGEPGGHWVNAGAFFGGMVLIGLIDHLIPSAENPHE
                     VHGAEESAPLRDPMAPLPDFSLPLGGTERALGRHDHGASHHRLIRMGLFTALAIGIHN
                     LPEGLATFLAALQDPKLGMAIAVAIAMHNIPEGVSVSVPIFYATGDRRRAFLLSLLSG
                     LAEPVGAVIAFVALRFFIGAGTGVIPPHAMGVLFAGVAGIMVYISVDELLPTSRAYGK
                     GHDSLLGVMGGMVMMALSLLLMK"
     gene            2540..3388
                     /locus_tag="PLQ54_01565"
     CDS             2540..3388
                     /locus_tag="PLQ54_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOS91978.1"
                     /translation="MQAFLDCVPCLVGQCLRAARLATSDPELQARALRAVMGYLQQAD
                     LSGSPIDISLEPHRLVREVTGCPDPYAALKAKSNSEALQLYGRLKELVRSSDHPLLTA
                     VRIAAAGNIMDFGALVSFDVETTLNRALTVPFGIDHSHEFLQAIASAHRVLYLLDNAG
                     EIVFDRILIEELAGKHVTAAVKSDRFINDAMLEDARAVGLDQVAEIVAVPPADISPAP
                     FAGAWEAGALIIAKGQANFEAFRDVDGPIFFLLIIKCPVLAADAGAQTGDLVLQSRAL
                     VHARRE"
     gene            3407..4510
                     /locus_tag="PLQ54_01570"
     CDS             3407..4510
                     /locus_tag="PLQ54_01570"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOS91979.1"
                     /translation="MPPATARAAVLASYGRPLELREFPLVPLADGEVRVRVMAAGVCG
                     SDVHIWRGEDPRTPLPLILGHEGVGCVDEIRGAKQDVYDQELRPGDLVLWERGITCGR
                     CAYCVIRKQPALCPHRRTYGISVGCADAPHLRGAYAEYIHLRADAHLIRVDGDVDPAV
                     LVSATCSGACAAHAIEQAHIRPGDAVLVLGPGPMAMFLVAFARERGAGHITVIGSRRS
                     AARLAMAQRMGADSVLFRDETSLADRLEAVRDQTHGFGACVVFEASGSPEAVAEAVEH
                     TAPYGTCLIPGIAVPVGRVPVSVFEHIARKNVRLQGVWVSDTSHLCAALDLVLSRRYP
                     FDEMVTHRFPLDEATGALEVVERREAVKAVVMP"
     gene            4666..5481
                     /locus_tag="PLQ54_01575"
     CDS             4666..5481
                     /locus_tag="PLQ54_01575"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOS91980.1"
                     /translation="MIVIGELINSTRKNVRPAIEQRDTEAIQKMARDQAEAGAHFIDV
                     NSGHFIEGEPELLQWLVQTVQAAVDLPCALDSPRASAIEAGLAVHRGQAMVNSITGES
                     ERMEALLPVIAKAKPKVVALCMDDTGIPGNAKARVAIACKLIDALAGVGVPHSDVFVD
                     PCVLPVSSADLEGKAEHPGEIVVNVMREIRARYPEVHLSGGLSNVSYGLPKRKLINRA
                     ACLVYMAAGMDAAIIDPLDEYLMSLVRAAECILNRDEMCMGYITADREGKLVV"
     gene            5562..>6449
                     /locus_tag="PLQ54_01580"
     CDS             5562..>6449
                     /locus_tag="PLQ54_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91981.1"
                     /translation="MPTSLLLATPLMACSMAVTAPLTTGDLVAEMVDLDRLSRFPDPA
                     YRTVQFSSHDRASVAPYAPGWYANSDGFGGEETPNFLEVIEPPNDDGIGRYLMAEVEG
                     PGAIVRTWSAAIAGEVAVYLDHSDEPLYQGPAEAFLMRMYDEWARQVGLPEEGPPGGF
                     QQAWSCYFPVPFAQAMRIEWTGRVRDIHFYHIEVRQYAAGTEVRTFSPSDLRTHQTAI
                     ERVRTMLRSPETQWPIQGRQAHIAVEVDPGSRAEALKLIQSSAMVVLLPQQHVHSIPG
                     KVFEYVQLAAWPLIIADTGT"
BASE COUNT         1082 a         2220 c         2072 g         1075 t
ORIGIN      
        1 cagcaacggc cccgacctcg agcctagagt cccaacgacc tgaggtgagc taggctgatc
       61 cgcgctgcct cggtcggatc cagcttgctc tggtcctgct cgtagacgat ccactgaggg
      121 tcgtgccgtc gagccgccgc cagcgctgct gccagatcca ccgagccccg gcccaactcg
      181 gtgaactcat agggccccgg agcgatcgaa cccgtcgggg tgtacggacc gtccttgaag
      241 tggtagtagc cgactcgatt gccgtaacgt tcgatgaact cctcgggctt ctccccgccc
      301 accgtcaccc agtacacgtc gacgttcagc ttgacgagat ccgggtccgt gacctcgcaa
      361 agcttgtgga tgcccttgac gccgtcgaaa acacggaact cccacgcatg gttatgataa
      421 aggaagttca agcccgcttc cttgcatcgc gcaccgaagg cattgaacac cgcccccgtc
      481 tcgatgtagc cctccaggct gtcgttgtcg gccacacccg agcagatgat gtgcttggcg
      541 cccatgtcct tgcagaactc gatggccttc gcttgccaac tggcatcggg gatcgagccg
      601 tagccgccat gaatgcccga cagcgcaatc cccgcgttgg cgaaggccgc cttgacctgg
      661 acccggtccg cgtccatgaa gttggtgccg atctccgctc ccgcgtagcc ggcctgggcc
      721 agctccgtca acactccggc caggtcaaac tcgggtcggc ctccgtagac gatcagctgc
      781 gctgcgatcc tgggattggc cacggtgatg cctccaagtg gaatgcccgc tgtcgggcaa
      841 gatcccccgg cgctccttgc gccgggtcag tcggcgaacg ggtcgggttt cgaccgagtg
      901 cggccgaaga cctggcagac aggaagcccg tccaacacgc ggcggattcg ggggcacact
      961 gccgccccag cggatcctgg cccgcagcac tactcggccg tctggtgcct cagcgcatgg
     1021 gcatcgcgtt cgcgcttgaa ctgggccaga accccgttga cgaacgtgcc ggaagccgaa
     1081 tccccatatc gtttggccag ctcgacggcc tcgttgatcg acacgttcag cggtatgtca
     1141 tcgcggtaac gcatctcgta cagggccaac cggaggacac accggtcgat ggacgccatg
     1201 cgatccaccg accaatccgt ggcatagtac gcgatcagcg cgtccagctc ttggacgcgc
     1261 tctgcgacac catgaaccac atcggccacg tagaggaccg cctcgtccgc ggggcgctcg
     1321 tcgctatagc gttgccagcg cacgagcgcc gactcgacgg cctcacggct gaacccgaag
     1381 acgacatcca gctgatagag gaggcgcagc gcctgcctgc gcgcctggcg acgggactgc
     1441 atggccccat tgtagcggcc cgagccggac ggtggccagt gcgccccgtg tcccgccggc
     1501 gccctacttc atcagcagca gactgagtgc catcatcacc ataccgccca tcacgccgag
     1561 caggctgtcg tgtcccttgc cgtaggctcg gctggtgggc agcagctcgt cgacgctgat
     1621 atagaccatg attccagcga cccccgcgaa cagcacgccc atcgcatgag gcgggatcac
     1681 gcccgtgcct gcgccgatga agaagcgcag cgccacgaac gcaatgacgg ccccgaccgg
     1741 ctccgccaag ccgctcagaa gagataagag gaaggcgcgc cggcgatcac cagtggcgta
     1801 gaagatgggc accgacacgc tgaccccctc ggggatgttg tgcatggcga tagcgacggc
     1861 gatggccata cccagcttgg gatcctgcag cgcagccagg aaggtcgcca gcccctcggg
     1921 caggttgtgg atcccgatgg ccagcgccgt gaagagcccc atgcgaatca gccggtggtg
     1981 tgatgccccg tggtcgtgcc gcccaagggc ccgctctgta cccccgagcg gcaggctgaa
     2041 gtccggaagg ggcgccatcg gatcgcgcag cggcgccgac tcctcggccc catgcacctc
     2101 gtgggggttc tcagccgagg gaatcaggtg gtcgatgagt ccgataagga ccataccgcc
     2161 gaagaatgcc ccggcgttga cccaatggcc gccgggctcg ccgtatgcct cgacgagcgc
     2221 gaccgtgccc ttgtggaaga tctcgacaaa cgagacgtag agcatgaccc ccgccgagaa
     2281 gcccgtcgcg accgacagga accggaagtt cgtcggtttg gcggcgaacg cgatcatgct
     2341 cccgatcccc gtcgccatac cggcgaacag ggtcagtccc agtgcgtaca gaacgccact
     2401 caagagcttg tctgcctcct ctcgccggct cgctggccgt ccgtccgatc ccgccggcga
     2461 actcccggcc tttcgcccgt atcccccggc gactagaatg catcgtctga tgccgtgcgt
     2521 ttggtagggc tgggggacca tgcaagcgtt cttggactgt gtgccatgtc tggtaggcca
     2581 atgcctccgc gccgcgcggc tggccaccag cgatcccgag cttcaggcgc gggccctccg
     2641 cgccgtgatg ggctatctgc agcaggccga cctctccggt agccccatcg acattagcct
     2701 ggagccccat cgcctggtcc gggaggtgac gggctgcccc gacccctacg cggcactcaa
     2761 ggccaagtcg aactccgagg ccctgcagct atacgggcgc ctcaaagagt tggtccggtc
     2821 cagcgatcac cctcttctga cagccgtccg gatcgcggcg gctggcaata tcatggactt
     2881 tggggccttg gtctccttcg acgtcgagac gaccctcaac cgggccctca ctgtaccgtt
     2941 cggcatcgac cactcgcacg agttcctcca ggctatcgcc tcggcgcacc gcgtgctcta
     3001 tcttctcgac aacgctggcg agatcgtctt cgaccggatc ctgatcgagg agctcgccgg
     3061 caagcacgtg accgccgcag tgaagtccga ccggttcatc aatgacgcca tgctggagga
     3121 tgcccgggcc gtcggcctcg atcaggtggc cgagatcgtc gccgtgccac cagccgacat
     3181 ctcgcccgcc cccttcgccg gggcgtggga ggccggcgcc ctcatcatcg ccaagggtca
     3241 agccaacttc gaggcattcc gtgacgtgga cggccccatc ttctttctgc tcattatcaa
     3301 gtgcccggtt cttgcggcgg atgccggggc ccagaccggc gacctggtgc tacagtcgcg
     3361 ggcattggtc cacgcccgcc gggaataggc aggaggagga ccgaccgtgc ccccagccac
     3421 cgctcgcgcc gctgtcctgg ccagttacgg ccggccgctg gagctgcgtg agttccccct
     3481 ggtacccctc gccgacggtg aggtgcgcgt ccgcgtcatg gccgcgggcg tgtgtgggtc
     3541 tgatgtgcac atatggcgtg gcgaggatcc gcgaacgccc ctcccgctga tccttggcca
     3601 tgagggtgtc gggtgcgtcg atgagatccg cggggcgaaa caggacgtct acgatcagga
     3661 gttgcggccg ggagacctgg tcctatggga gcgcggcatc acgtgtggcc gctgcgccta
     3721 ctgcgtcatc cgcaaacagc cggccctgtg ccctcaccgg cggacgtatg gcatctcggt
     3781 gggttgcgct gacgctccgc atctccgcgg cgcctacgcc gagtacatcc acctgcgggc
     3841 cgacgcgcac ctgatccggg tcgacggcga cgtggatccc gcggtcctgg tgtcggccac
     3901 ctgttcgggc gcctgcgcag ctcacgccat cgagcaggcc catatccgtc ccggcgatgc
     3961 ggtactggtt cttggcccgg gccccatggc catgttcctc gttgcattcg cccgcgaacg
     4021 cggcgcgggc cacatcaccg tgatcggcag ccgccgcagc gccgcaagac tcgcgatggc
     4081 ccagcgcatg ggggccgact cggtgctctt ccgcgatgag acgtcgctcg cagaccgcct
     4141 ggaggcagtt cgcgaccaga cacatggctt cggggcctgc gtcgtcttcg aggcttcggg
     4201 gagccccgag gccgtggccg aggccgtgga gcacaccgcg ccgtacggca cgtgcctcat
     4261 tcccggcatc gccgtgcccg tcggccgcgt gcccgtctcg gttttcgagc acatcgcgcg
     4321 gaagaacgtt cgcctccagg gcgtatgggt gagcgacacg tcgcacctgt gtgccgccct
     4381 ggacctggtg ctgagtaggc gctacccgtt cgacgagatg gtcacgcacc ggttcccgct
     4441 cgacgaggcc accggggccc tcgaggtcgt cgagcgccgc gaggcagtca aggccgtggt
     4501 gatgccctga ccggcactgg ggcggcgcga gtcggcggat tccggacacc tttgtgcgcc
     4561 gggcataggg gccggcacac ccacggccga atagtcatgg ccggctcact ctcacgggcc
     4621 aacactgggc ccgccaacca tccgggccac gggaggcgcc gtttcttgat cgtcatcggc
     4681 gaactgatca acagcacccg caagaacgtg cggccggcca ttgagcagcg cgacaccgaa
     4741 gccatccaga agatggccag ggatcaggcc gaggcagggg cgcacttcat cgacgtgaac
     4801 tcggggcact tcatcgaggg tgaacccgag ctgctccagt ggcttgtgca gacggtgcag
     4861 gcagctgtcg atctgccgtg cgcgctcgat tcccctcgcg catcggccat cgaagccggc
     4921 ctcgcggtgc accgcggcca ggccatggtg aactcgatca ccggcgagag cgagcggatg
     4981 gaggccctgc tgcccgtcat cgccaaggcc aagcccaagg ttgtggccct gtgcatggac
     5041 gacacgggta ttccgggaaa cgcgaaggcc cgggtcgcca tcgcctgcaa gctgattgac
     5101 gccctggctg gcgtgggcgt gccgcacagc gatgtctttg tcgatccctg cgtgctcccg
     5161 gtgagctcgg ccgacctcga aggcaaggcg gagcacccgg gcgagatcgt ggtgaacgtc
     5221 atgagggaga tcagggcccg gtatcccgaa gtccacctct cgggcggatt gtccaacgta
     5281 tcgtacggcc tccccaagag gaagctcatc aaccgcgcgg catgcctcgt ctacatggca
     5341 gccggcatgg acgccgcgat catcgatccg ctggacgagt acctgatgtc cctggtgcgc
     5401 gctgccgaat gcatcctcaa ccgtgacgag atgtgcatgg gctacatcac ggcggaccgc
     5461 gaggggaagc tcgtggtgtg agacccttga gctgatggtg gctctgtcat cggcgccgga
     5521 gcgcgtacac gccccggcgc cattcgatgc aggaggacct aatgccgact tccttgctgt
     5581 tggccacccc gctcatggct tgttcgatgg ccgtgaccgc gcccctcacg acgggcgacc
     5641 tggtcgccga gatggtcgac ctcgaccgcc tgtcgcggtt cccagacccg gcataccgga
     5701 cggtgcagtt ctcatcccat gaccgggcca gcgtcgcgcc gtacgccccc ggatggtacg
     5761 cgaacagcga cggctttggt ggcgaagaaa cacccaactt cctcgaggtg atcgaaccgc
     5821 cgaacgacga cgggatcggc cggtacctca tggccgaggt cgaaggccct ggtgccatcg
     5881 tccggacttg gtcggcggcc attgccgggg aggtcgcggt ctacctcgac cacagtgacg
     5941 agccgctcta ccaaggcccg gccgaggcgt tcctgatgcg gatgtatgac gagtgggccc
     6001 ggcaggtcgg actgcccgag gaggggccgc ctggcgggtt ccagcaggcc tggtcgtgct
     6061 acttccccgt gccgttcgcg caggccatgc ggatcgagtg gaccggccga gtgcgtgaca
     6121 ttcacttcta ccacatcgag gtgcgccagt acgctgcggg cacagaggtt cgcacgttct
     6181 caccctccga cctgcgaacg caccagaccg ccatcgagcg cgtgcgaacc atgttgcgct
     6241 cgcccgagac ccaatggccg atccaggggc ggcaggccca catcgcggtg gaagtcgatc
     6301 ccgggtcgcg cgccgaggcg ctcaagctga tccagtcgtc ggcaatggtc gttctcctcc
     6361 cacagcagca cgtgcactcg attcccggca aggtcttcga atacgtgcag ctggcggcgt
     6421 ggccgctgat catcgccgac accggcact
//
LOCUS       DAOSPL010000048         6427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_12168_length_6427_cov_2.960923, whole genome shotgun sequence.
ACCESSION   DAOSPL010000048 DAOSPL010000000
VERSION     DAOSPL010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12168_length_6427_cov_2.960923"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2127)
                     /locus_tag="PLQ54_01585"
     CDS             complement(<1..2127)
                     /locus_tag="PLQ54_01585"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOS91982.1"
                     /translation="MARRLAGLAALTAICLGVACGCGKSAATRAAETRFTPSDYASVM
                     AGVSADRMGEDIRYFASLGSRLTGYPGADKAAQYVERQFRGAGIQNIEYQDFEIMVPV
                     DHGSTLIVDGEPIPIHAVWPNVVRTSQVAPGTLRGPVVYGADCSLGRFDGAAMDGAIV
                     LADFNCGTNWLNVPLLGGRAVVFVEPRDTTWSDAAEKYLKVPVDVPRFYIEREQADPL
                     LARLKAAQGSSAPSGGIECRMTWERRKGRNIIATLPGSDPELSAEHIFLEAYYDSISV
                     VPNRAPGADQASSIAALMELARLLAAAPPKRTVVLAACSGHFEAMRGAHRLGDVLGPK
                     GRRPREQDQFLRDGIRERNDTLRWLRRSAQRAAQRRDFLEAERKAGKFELTARGDIDW
                     DAALALTQDRIDLVNQELALVRKYRDRQAVAAIPLWLSLDLSSHSRRLGLNHCGWYYN
                     RGDLIRFFSPIGKRMGEYLEGASKALGYEKPDLFWFDGTNPKQNVSWPDYFPGRIAFD
                     SEAPLQAGRPACVLATGYDQRPYFNTPLDTVERMDMDGLTFQTRVIACILSDFLNDSA
                     LTETALKRTHRLKLPYRLEPLRGNVLEFVHKKSFLPDTPVPGCLTLIAGPFKSMMGVH
                     NDVMDLTDGSGAFSIQGYNAIGWGNVDAYYIDPVSGDVIYAPDLGSEGEKRYTRNVGK
                     RGRQIRPVIVFPCRPIDFYDLVDQRYL"
     gene            complement(2270..2833)
                     /locus_tag="PLQ54_01590"
     CDS             complement(2270..2833)
                     /locus_tag="PLQ54_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOS91983.1"
                     /translation="MMADAPLSRLIEPSSALLMVIDLQEKLVPHIHQSDRVVERAVRM
                     IRAARILGVPVVVTEQYPRGLGPTVAPVLEVVPDVKMEKTTFGCLGTPGIRDAVVAPG
                     VRTVILVGIEAHVCVLQTALQALSEGLAVHVVRDAVGSRAIEDADAGLARMSQAGASI
                     TTSEMTILELVGDAKRPEFRQVLPLVR"
     gene            complement(2937..4502)
                     /locus_tag="PLQ54_01595"
     CDS             complement(2937..4502)
                     /locus_tag="PLQ54_01595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOS91984.1"
                     /translation="MGRTPGAPPAGLMSRATVYGWAAILLVGLLLRVPGLTWGLPDEH
                     KLFPYHPDEPTLVELGSRMARTGDLNPHFFNYGSLPVYLSALVLGAVGLSAAPPGDSG
                     PYLALRLVSLVFGLGTIVLAGALAGRLLGKREQWVAAGLVAVTPIHVIGSSYATVDSL
                     ATFLLAAAVLCGVRVLDGSRPADYALAGAFAGLAASTKYVGLTAALVPLACAAWRERR
                     WLPSRSSWAGLGIAVACATGAFALTTPYAFIDRSSFWRDFAFELQHKREGGTTVPPGV
                     SGFAYLFGTALPSGLTWPLVVVMVAGLLLALRRRDRSVPLAAWLVWLVIVTGSGREVF
                     VRYTMPLAPFAAVVVAGLCGGLRPGHAGVALGLVAAVGLYALAYSAAYTGMYRRPDVR
                     DAALDHIVAQAENGHPSVGLVQAPWFFSPPIAGLNGGPRTAGVYGEWHASGAGRSPSR
                     LGIPRDLRWRGPTCLSSPSSSSRRMPGSGQRAPPSSSVSSSATTGSAPSSDPTRCSWA
                     SVSPTLMPTIGAT"
     gene            complement(4495..>6427)
                     /locus_tag="PLQ54_01600"
     CDS             complement(4495..>6427)
                     /locus_tag="PLQ54_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91985.1"
                     /translation="FGLSGFMVVWLCYVTPVAAVTWMPAALWGIDRLARRRKVSDAVL
                     LAGAVAMTFLAGHGQISLYVMLTTLAYGLYRVGGLLRTEGRTEAFWLVCLLAGGLLTG
                     TSLAAAQLLPTLEFGRINYRTGAIPYESLIGIQPAQLMTLLVPDAFGNPADYTDEVGR
                     QMGLGVNHFIEGCAYPSCVLAALALVGLAAGRRPERWFLAGATGVAVLLAMGSPLNRL
                     TYYLVPGMNQMPNLGRVLCVACLTIPILGAMGLEAVLDETAPAMRRRMGRALLVSMAL
                     VAVVGLRCLLPIVMDSEAAGFPRSVLTAVGGSDALVRVQGRIAMCIGLAATILGLTAA
                     RLARRVAPRPLVAAGVTLVVVDMLAFGQRFEPACDPVIIETRQPALEALRADRPQRIA
                     ALGPVARTGSGEDTLARLSPNVGMTYGLAEVRGSESLYTKRYDEVLRHLALAPPYEAL
                     SDGRAKLLDVLGVRHLVARGAFPGTEPVEGTSLVHRRPSAFPPASVVPRARPVSDQVA
                     ALACFDEAAFDPRAEVPIEGHAAGEAESGRGCAPAAVQVRTNSVRARAGGAGWLVVSQ
                     AAFPGWRAYLDGSPSPLYVAYGVVWATPVPASGATVDLVFVPSTSLVGQFVSLTTLAG
                     LLAVVVATARRRDGEPGHG"
BASE COUNT         1137 a         2217 c         2172 g          901 t
ORIGIN      
        1 caggtagcgc tggtcgacca ggtcgtagaa gtctatgggc cggcaaggaa acacgatcac
       61 cggccggatc tggcgcccgc gcttacccac attgcgcgtg tagcgcttct cgccttcgga
      121 tccgagatcg ggcgcgtaga tgacgtcgcc actcacggga tcgatgtagt aggcgtccac
      181 attgccccag ccgatggcgt tgtagccctg gatggagaac gcccccgagc cgtcggtgag
      241 gtccatcaca tcgttgtgaa cacccatcat gctcttgaac ggcccggcga tgagcgtcag
      301 gcagccggga accggcgtgt cgggcaggaa gctcttcttg tggacgaact cgagcacgtt
      361 cccgcgcagc ggttcgaggc ggtagggcag tttgagccgg tgggtacgct tgagcgccgt
      421 ctcggtgaga gctgagtcgt tgagaaaatc gctgaggatg caggcgatga ctcgcgtctg
      481 gaaggtcaga ccgtccatgt ccatccgctc gaccgtgtcc agaggcgtgt tgaagtacgg
      541 gcgctgatcg tacccggttg ccagcacaca ggccgggcgt ccggcctgga ggggcgcctc
      601 gctgtcgaag gcaatgcgtc cggggaagta gtccggccag ctcacattct gcttcggatt
      661 ggttccgtcg aaccagaaca ggtcgggctt ctcgtacccg agggccttgg aggccccctc
      721 aaggtactca cccattcgct tgccgatcgg agagaagaaa cggatcaggt cgccccggtt
      781 gtagtaccac ccgcaatggt tcaggcccag tcgtctgctg tgactgctca ggtcgagcga
      841 tagccacagc ggaatggctg ccaccgcctg gcggtcgcgg tacttcctca ctagggcgag
      901 ctcctggttc accaggtcga tgcggtcctg ggtcagcgcc agcgcggcgt cccagtcaat
      961 gtcgccccga gccgtgagct cgaacttgcc ggccttcctt tcggcttcga ggaagtcccg
     1021 tcgctgcgcg gcgcgctgag ccgatcgccg gagccaccgg agtgtgtcgt tgcgctcgcg
     1081 aatgccgtct cgcaggaact ggtcctgctc gcgcgggcgc cggcccttgg ggcccaggac
     1141 atccccaagt ctgtgggccc cgcgcattgc ctcaaagtgc ccggagcagg ctgccagcac
     1201 cacagtccgc ttcggagggg ccgctgccag cagtcgagcc aactccatga gggccgcgat
     1261 gctgcttgcc tggtcggccc ccggcgcccg attcggcacg acggagatcg agtcgtagta
     1321 ggcttcgagg aagatatgct cggctgagag ctccgggtcg ctgcccggca gtgtcgcgat
     1381 gatgttgcgg cccttccggc gctcccacgt catgcggcac tcgatgcccc cggacggtgc
     1441 cgaagatccc tgggccgctt tgagcctcgc gagcagcggg tctgcctgct cgcgttcgat
     1501 gtagaaacgc ggaacatcaa cgggaacctt caggtacttc tccgccgcgt cggaccacgt
     1561 ggtatcccgc ggctcaacga ataccacggc ccggccgccc aggagcggga cgttcaacca
     1621 gttggtgccg cagttgaagt cggccaggac aatggcgcca tccatggcag ccccgtcgaa
     1681 gcggccgagg ctgcagtcgg caccatagac cacggggccc cggagggtgc ccggtgccac
     1741 ctgagaggtg cgcaccacgt ttggccagac cgcgtgaatc ggtatgggct cgccatcgac
     1801 tatcagggtg gacccgtggt ccacgggcac catgatctcg aagtcctggt actcgatgtt
     1861 ctggatcccg gcgccgcgga actgccgctc gacatactgc gccgctttgt cggcacccgg
     1921 gtagccggtc agccggctgc ccaggctcgc gaagtagcgg atgtcctcgc ccatgcggtc
     1981 ggccgagacg ccggccatga ccgacgcgta gtcgctcggc gtgaagcgag tctccgcagc
     2041 gcgggtcgcg gcgctcttcc cgcagccaca tgccacgccc aggcagatcg ccgtcagtgc
     2101 ggcgaggccc gccaagcgtc tagccatagc agcgtgcctc cggccttcgt tggaaatcat
     2161 cgaacccagc cgaacgcgcc ccaccggccc gagcgttcag gccgcctcgt cggtccttcg
     2221 cgatccgatg cccgaggccc ttcaccaggg ggaccagacg gcgtaccggc tacctcacca
     2281 acggcaacac ctgtcgaaac tccggccgct tcgcgtcgcc caccagttcg aggatggtca
     2341 tctccgacgt cgtgatcgac gctcccgctt ggctcatccg cgcgaggccc gcgtcggcgt
     2401 cctcgatggc acgcgagccc accgcatcgc ggaccacgtg gaccgcaagc ccttccgaga
     2461 gcgcctgcag agcggtctgg agcacgcata catgggcctc aatgccgacc aggatcacgg
     2521 tccggactcc gggagccacc accgcatcac ggatgcccgg cgtgccgagg caaccgaagg
     2581 tggtcttctc catcttcacg tcggggacta cctcgagcac gggcgccacg gtgggcccca
     2641 gaccacgtgg gtactgctcg gtgaccacca cgggcactcc caggatccgg gccgcgcgga
     2701 tcatgcgaac cgcgcgctcg accacccgat cgctctggtg tatgtggggc accagtttct
     2761 cctggaggtc aatgaccatc agcagggcgc tcgagggctc aatcagccga gagagcggag
     2821 cgtccgccat catgtccgac caccgtcctt ccaccgagtg gcggttcatt cagagggggc
     2881 gggcaacgcc cctgccgtcg gaggcgtctg tcttcggtag acgcggatct cggggttcat
     2941 gtagcgccaa tcgtgggcat gagagtcggg gaaacggatg cccaggagca tcgggtcgga
     3001 tcggaggacg gcgctgagcc ggtagtggcg ctcgaggaaa cggacgaact cggtggagcc
     3061 cgttggccgg agcctggcat cctgcgggaa ctcgaactcg gagaggacaa acacgtcggg
     3121 ccacgccacc gcaagtcgcg agggatccca agccgtgatg gtgacctccc agccccgctc
     3181 gcgtgccact ccccgtagac tccggccgtc ctgggtccgc cgttcaagcc ggcgatgggg
     3241 ggactgaaga accacggagc ctgcacgagt ccgacggacg ggtgcccgtt ctcggcctgg
     3301 gccacaatgt ggtccagggc cgcatccctc acgtccggcc gccggtacat acccgtgtag
     3361 gctgccgagt aggccaaggc gtagagcccc accgccgcga cgaggcccag cgcgaccccg
     3421 gcgtgcccgg gcctgagccc gccgcagagc ccggccacta ccaccgccgc aaatggtgcc
     3481 aacggcatag tgtagcgcac aaacacctcg cggccactcc cggttacgat gaccagccac
     3541 acgagccagg cggcgagggg aacggagcgg tcgcgccgtc gcagcgccag caagagacct
     3601 gcgaccatca ccacgaccag cggccatgtg agaccggagg ggagcgccgt cccgaacagg
     3661 taggcgaagc cgctgacgcc gggtggtacc gtggttccgc cctcgcgctt gtgctggagc
     3721 tcgaaggcga agtcgcgcca gaagctcgag cggtcgatga acgcataggg ggtggtgagt
     3781 gcgaaggcgc cggtggcgca ggcgaccgcg atacccaggc ccgcccaact cgacctagac
     3841 ggaagccacc gccgctcccg ccaggcggca caggcgagcg gcacgagcgc cgccgtgagg
     3901 cccacgtact tggtgctcgc cgccaagccc gcgaacgcac cggccagagc gtagtcggca
     3961 ggccgagatc catcgaggac ccgcaccccg cagagcacgg cggccgcgag caggaatgtg
     4021 gccagcgagt ccaccgtggc ataggacgag ccgatcacgt ggatgggcgt gacggccacc
     4081 agcccggccg cgacccattg ttcacgcttc ccgaggagcc gaccggccag ggctcctgcc
     4141 agcacgatgg tgccgagccc gaagaccagt gagaccaggc gcagggccag gtaggggccc
     4201 gagtcaccgg gcggcgcggc actgagtccg acggcgccca gcacgagtgc ggagaggtac
     4261 accggaagcg agccgtagtt gaaaaagtgg gggttgaggt cccccgtccg tgccatgcgc
     4321 gacccgagtt cgacgagcgt gggttcgtcg gggtggtagg gaaacagctt gtgctcgtcg
     4381 ggcagtcccc aggtcagacc cgggacgcgg agcagcaggc ccacgagcag aatagccgcc
     4441 cagccataca ccgtggcgcg cgacatgaga ccagcgggcg gagcacccgg ggtcctaccc
     4501 atggcccggc tctccgtccc ggcgccgggc ggtggccacg accaccgcga gaaggcctgc
     4561 cagggtggtc aacgacacga actgacccac caggctggta ctgggtacga agaccagatc
     4621 gaccgtggcg ccactggcgg gcacaggtgt ggcccacacg acgccatagg ccacgtagag
     4681 aggcgacggg cttccatcga ggtaggctcg ccagcccgga aacgccgcct gtgaaacgac
     4741 cagccacccc gccccgccgg cccgggcacg caccgagttg gtccggacct ggaccgcggc
     4801 gggcgcgcag ccacgtcccg actccgcttc gccagccgcg tgtccctcga tgggcacttc
     4861 ggcccgcggg tcgaaggcag cctcatcaaa gcacgccagg gcagccacct ggtcactcac
     4921 gggccgagct cggggcacga ccgacgccgg agggaacgct gacggccgac ggtgcaccag
     4981 cgacgtgcct tcgacgggct cggtacccgg gaacgcccct cgggccacga ggtgccgcac
     5041 tccgagcacg tccagtagct tcgctcgccc gtcggacagg gcctcgtagg gcggagccag
     5101 cgccagatgg cgcagcacct cgtcgtaccg cttggtgtac agcgactccg agccacggac
     5161 ctccgcaagg ccgtaggtca tcccgacgtt cggcgagagg cgggccagcg tgtcttcccc
     5221 cgagccagtg cgtgccaccg gcccgagggc cgcgatgcgc tgcggccggt cggcacggag
     5281 tgcttccaag gcgggttgcc gcgtctcgat gatgacggga tcacacgccg gctcgaagcg
     5341 ctggccgaat gccagcatgt cgacgaccac cagcgtgacc cccgccgcaa cgaggggccg
     5401 gggcgccacc cgtcgggcga gtcttgcggc cgtcagcccg aggatcgtgg cggcgaggcc
     5461 gatgcacatg gcgatccgtc cctggacccg caccagcgca tcgctgccgc cgacggcagt
     5521 caggaccgac cgtggaaacc cggcggcctc ggagtccatc acgatcggaa gaagacaccg
     5581 cagccccacc acggcgacca gcgccattga gacgagaagc gcacgcccca tccgccgccg
     5641 catcgcgggg gccgtctcgt ccagcaccgc ctcgagtccc atggccccca ggatcggaat
     5701 ggtcaggcag gcgacacaca gcactcggcc caggttgggc atctggttca tgcccggtac
     5761 cagataatag gtgagccggt tcaggggcga gcccatggcc agcaggaccg ccacgccggt
     5821 ggcgccggcg aggaaccagc gctcgggccg gcggccggcg gcaaggccta ccagagccag
     5881 agcggccaga acgcagctgg ggtaggcgca tccctcgatg aaatggttca cgcccaaccc
     5941 catctggcgg ccgacctcgt cggtatagtc ggctgggttg ccaaaggcat ccgggacgag
     6001 caacgtcatg agctgcgcgg gctggatacc gatgagtgac tcatagggga tggcaccggt
     6061 ccggtagttg atccgcccga actcgagcgt gggcagcaac tgggccgcgg ccagtgaggt
     6121 gcccgtcagc agcccaccgg ccagcaggca cacgagccag aaggcctcgg tgcgcccctc
     6181 ggtgcgcaga agcccgccga cgcggtagag cccgtatgcc aacgtggtca gcatcacgta
     6241 caggctgatc tggccatgcc cggcgagaaa ggtcatcgcc acggcgcccg caaggaggac
     6301 ggcatcggat accttccggc gccgcgccag ccgatcgatg ccccagagcg ccgccggcat
     6361 ccacgtcacc gccgccacgg gcgtgacgta acacagccac accaccatga agccactcag
     6421 gccgaag
//
LOCUS       DAOSPL010000049         6380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_12287_length_6380_cov_2.754466, whole genome shotgun sequence.
ACCESSION   DAOSPL010000049 DAOSPL010000000
VERSION     DAOSPL010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12287_length_6380_cov_2.754466"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLQ54_01605"
     CDS             complement(<1..403)
                     /locus_tag="PLQ54_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91986.1"
                     /translation="MGRFVNALTVLATLTGVLLALIFVPMRREHKAGTLMTPERKDFW
                     TRRMALWVAYMFLGLATLGKGLLGFMLPGAILVLYLLITTEWKALKRLEILRGLLFMC
                     LVMLPWYLGMFAKHGNAFYNRFLVHDHFNRLG"
     gene            complement(453..854)
                     /locus_tag="PLQ54_01610"
     CDS             complement(453..854)
                     /locus_tag="PLQ54_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91987.1"
                     /translation="MAVTRVLAVAAVALLVTGTGRATAMEKATDTELTGISGAGQCQH
                     YVDQECKAKGASGPCPFSGCNSDEVEYYRTAVPIQRPGCVKNAFTVGKRCTDEYLERD
                     LCTINYWDSANCTTDWHHSSVHRGGEWGCTR"
     gene            complement(926..1156)
                     /locus_tag="PLQ54_01615"
     CDS             complement(926..1156)
                     /locus_tag="PLQ54_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91988.1"
                     /translation="MGRPMGGPGRREFLRNAGTTGLLAFVGMSSFDALLGQISQRADE
                     RSAARGIAEALRTTSVASAEGDYCVLIEDPYE"
     gene            complement(1187..2326)
                     /locus_tag="PLQ54_01620"
     CDS             complement(1187..2326)
                     /locus_tag="PLQ54_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91989.1"
                     /translation="MGTGKPAGPVPRRTLIRTLCFLFALCTSPLLAQGPHLLRLEPPG
                     MDVSAVLKTIADEKLPVGGIIIDVARSGQTLFPVPDSVFTTRVGFEDGTQLTRWIDQI
                     HAQGLKAYAGLDAVRWWTAAYADPDPFLERPELAELDSDLSCNPANDAKYASPWHPAV
                     RSSLVSLARELGSSYQALDGIIVCLRLSPSAYTGFSDAARRACIDALRIDPLDLAVFG
                     VSEDWDPNLKAWMEWRPRALTDLMRELTSAYRDADGTGQDRVIAIGSGAYPAYSLRLQ
                     ACCADDWLTWALDGLIDGVALDTVPGRERWHEELAVGLDVLERARLGIAPYIAIRGEH
                     HGSVVPLTDAYGQVAGAGLGGRALLLDPVVASELESAIGLLRSRR"
     gene            complement(2708..4105)
                     /locus_tag="PLQ54_01625"
     CDS             complement(2708..4105)
                     /locus_tag="PLQ54_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91990.1"
                     /translation="MDPVRVRLLLVLSAVLFAAQAPARGDFVGYSGLEPYRGVLVQTR
                     LQGLGTGASMADVVEALARAGVHAVCTAPAAETLLSSLGSGPERTLAETLELFVKAGG
                     LVGVNDGVVLLRHDRRAAGGEGWHSLLTTIGRADALAAILNALPAPTQAGVARGLPLL
                     DGVQGWEEASSAYDLLRGLGQDLGQVRAELRRRYGARETRLLLAFDPAFVLAPRIGAR
                     PQAPVQLVPPGAIMDVLQAATEPADAVMQRWAVPPDPLVEEWIREEAARDLAAPQPVA
                     RSVRIGQTAPMTLAEVAEAVSTPGTLRQAADGGIAQLGVLVGEGTYQVGSLAAAAAAI
                     WEVGAAWTPEGRVAFVPGRARLPRCLLAQWHFRPDLGAIPVALDDILAAKSLPAADLT
                     EEQWAYLEQRLADSTPPPAQAILADRQSLVLRLRPMVSETECPIRRFRELGSPETVID
                     VPETAQSALYGWPLT"
     gene            complement(4144..4635)
                     /locus_tag="PLQ54_01630"
     CDS             complement(4144..4635)
                     /locus_tag="PLQ54_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91991.1"
                     /translation="MRRSAPALLVLGLVAGLAGGSWADPLDPDCCTFETSNCDDWERY
                     GSQLYRSLGAYPYDTEIHTKLVEGIQPDRSWECENLRNEDGCHSQQSDPTIPPEIDVL
                     KTFSLSPEYGPLIQVTWRTRFEWQQCFHVWAIYNGVPEETWCLNQENSLPLPGQILQA
                     GVY"
     gene            complement(4908..6224)
                     /locus_tag="PLQ54_01635"
     CDS             complement(4908..6224)
                     /locus_tag="PLQ54_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91992.1"
                     /translation="MGTIAYSRLVVAWLALLCLGGGAARSEGPVTVRDILAALAQTAD
                     CVVACGTEEDLGRTSALPLLEGRDAPSTTRMLAALRCAGATIVARDSRYAVSFVREAR
                     ARERRGVGSSIRRTNLLLALLELLDPATRRAVLLGCYVRPFDLGPEATAVAIELARTD
                     DRPSPFWSSADAAAGAVLRLTIRLRLEVRGEADDRLGALTAGGGTAFVPYNDLVRRAG
                     LGPSPVWLGPGPQPAVEEAILGRAEPLESLSIPVVDAPPQAKLATPLHGVAALSFSEP
                     EGPWAAGRLVPFADVVSRCVRQDAVEWTCDPRLEPRAVYVCSRNASPEAVLETACAAT
                     WARPRLVGAHLHLGVGDARPSGTDGASGEPSEWLRLLIRELAEPALALQDLVARQRGK
                     PSLIWLLARPGAGLTGLWSPEAVREAEGQLSILRAPANAGATAEAE"
BASE COUNT         1065 a         2272 c         2079 g          964 t
ORIGIN      
        1 cgcccaggcg gttgaagtgg tcgtggacca ggaagcggtt gtagaaggcg ttgccgtgct
       61 tggcgaacat gccgaggtac cacggcagca tgaccaggca catgaagagc aggccgcgca
      121 ggatctcgag gcgcttcagg gccttccatt cggtcgtgat caggaggtac aggaccagga
      181 tggcgcccgg cagcatgaac ccgagcaggc ccttgcccag cgtggccagg cccaggaaca
      241 tgtaggcgac ccacagggcc atgcggcggg tccagaagtc cttccgttcc ggcgtcatca
      301 gcgtgccggc cttgtgctcg cgccgcatgg gcacgaagat gagcgccagc agcacgcccg
      361 tcagagtcgc gaggacggtc agggcgttca cgaatctgcc catcacacga tcactcctcc
      421 gctgtctccc gacacacgcg gcgccgctgc ggctaccggg tacagcccca ctcgccgccg
      481 cgatggaccg acgagtggtg ccagtcagtg gtgcagttgg ctgaatccca gtagttgatg
      541 gtgcaaagat cgcgctccaa gtactcgtcc gtgcacctct tgccgacggt gaaggcattc
      601 ttgacgcatc ccggccgctg gatcgggacg gctgtgcggt aatactccac ctcgtcgctg
      661 ttgcagcccg agaacggaca ggggccagac gcgcccttcg ccttgcactc ctgatcaacg
      721 tagtgctggc actggcccgc acctgagatg cctgtcagct cagtgtctgt tgccttctcc
      781 atcgccgtcg ctcggcccgt tcctgtcaca agcagcgcca ccgcagcaac agcgagcaca
      841 cgagtcacag ccattcggtt ggctcctctc cgacacacaa ccgctgctca tccgcctggc
      901 ggacggccct cgtgggcagg cgctgctact cgtaggggtc ctcgatcagc acgcagtagt
      961 caccttcggc actcgctacc gacgtcgtac gtagcgcctc ggcgatgcct ctcgccgcgc
     1021 tccgctcgtc ggcccgctgc gagatctggc ccaagagcgc atcgaagctc gacatcccga
     1081 cgaacgcgag cagcccagtg gtcccggcgt tcctgaggaa ctccctccga cccggaccac
     1141 ccatcggccg tcccatagac ctcccctcct ctctgttggc atttggttac cgccgcgagc
     1201 gcagcagtcc gatcgcggat tccagctcac tggcgaccac agggtcaaga agcaaggcac
     1261 gcccccccaa cccggcgccc gcgacttgcc cgtacgcatc ggtcagtggg accacggatc
     1321 catggtgttc gccgcgtata gcgatgtaag gggctatccc cagccgagcc cgctccagga
     1381 cgtccaagcc gaccgctagc tcctcgtgcc atcgctcgcg gccgggcact gtgtccagcg
     1441 ccacgccgtc gatgagcccg tcgagcgccc aagtcagcca gtcgtcggca cagcatgcct
     1501 gcaggcggag tgagtaggcc gggtacgcgc ctgagccgat ggcgatgaca cgatcttgcc
     1561 cagtaccgtc cgcgtcacgg taggcggacg tcagctcccg catcaggtca gtgagggctc
     1621 gaggccgcca ctccatccag gccttcagat tcgggtccca gtcctcagat acaccgaaca
     1681 cggccaggtc gagaggatcg atccgcaagg cgtctatgca tgctctgcgc gccgcgtcgc
     1741 tgaagccggt gtacgcggat ggcgagagcc gcaggcagac gatgatgcca tcgagtgcct
     1801 ggtacgagga gcctagctcg cgagctagag agacgagact ggaccgcact gcagggtgcc
     1861 acggggaggc gtacttcgcg tcgttggcgg ggttgcagct caagtcggaa tcgagctccg
     1921 cgagctcagg gcgctcgagg aaggggtcgg gatccgcgta cgctgcagtc caccaccgaa
     1981 ccgcgtccaa gcccgcgtag gctttcagcc cctgtgcgtg tatctggtcg atccaccgcg
     2041 tgagctgggt gccgtcctcg aagcccacgc gtgtcgtgaa gacgctgtcg ggcaccggga
     2101 agagcgtctg gccgctgcgg gccacgtcga tgatgatccc gccgacgggg agcttctcgt
     2161 cggcgatggt cttgagcacg gccgagacgt ccatgccggg cggttcgagg cgcagcaggt
     2221 gtgggccctg cgcgagcagc ggcgatgtgc agagggcgaa gaggaaacac aacgtgcgga
     2281 tcagcgtccg ccgcggaacc ggtccggctg gtttgcctgt gcccatggga tgccccccgg
     2341 tagtagcaca gcaccaaccc caacagccga ccacacacta gcccgtcagt gcgcccccgt
     2401 caagccctgt gagaaaaaag tacggcgaat acttgccgtg aaaacgctat caatagggga
     2461 gggcatatga accatcgctc ccggccccca tcccgcgccc tccattgccg ctgaagacgc
     2521 agatgcacct tggccgcatc gggttgggtc ctctactcgg tcggagccgg caggtgccgg
     2581 atcttgtcgt gcgaggccgc gaagtcgaac cccgccggct ggcgcgagtc acccacctcc
     2641 ccgccgcggc acactcggct gctttcgcag cgagaccggg ccgaccggat aacgctgcac
     2701 gtccacgtca cgtcagaggc cacccgtaca gcgcgctctg cgcggtctcc ggcacgtcga
     2761 tgactgtctc tggactgcca agctcgcgga accgcctgat cgggcactcc gtctccgaca
     2821 ccatgggtct gagacgcaac acgaggctct gacggtcggc gaggattgcc tgggcgggcg
     2881 gcggcgttga gtccgcgagc cgctgctcga ggtaggccca ctgctcctct gtgaggtcgg
     2941 ccgccggcag cgacttggcc gccaggatgt cgtccagcgc gacggggatc gcgccgaggt
     3001 cgggccggaa gtgccactgg gcgaggaggc accggggaag gcgtgcccgc ccgggcacga
     3061 aagccacccg gccctcgggg gtccacgcgg cacctacctc ccagatcgcg gcagcggccg
     3121 cagcgagtga tcctacctga tatgtgccct caccaaccaa gacgccgagc tgagcgatcc
     3181 ccccgtccgc cgcctggcgc aatgtgcccg gtgtgctcac ggcctcggcg acctcagcca
     3241 gggtcatggg cgcggtctgg ccgatcctca cgctccgcgc caccggctga ggggcggcga
     3301 ggtctcgtgc agcttcctcc cggatccact cctctaccag cgggtcgggc gggaccgccc
     3361 acctctgcat caccgcgtcg gccggttcag tcgcggcctg cagcacgtcc atgatggcgc
     3421 cgggcgggac gagctggacg ggcgcctgcg gacgcgcccc gatgcggggg gccagcacga
     3481 acgccggatc gaacgccagg agcagccgcg tctctcgtgc cccgtacctc ctgcgcagct
     3541 cggcccgcac ctgtcccagg tcctgcccca acccgcgcag cagatcgtac gcgctggacg
     3601 cctcttccca tccctggacc ccatctagca gagggagacc tcgagcaacg ccggcctgcg
     3661 tgggtgctgg caaggcgttg aggatggcgg ccagcgcatc ggccctcccg atggtcgtta
     3721 ggagcgagtg ccagccctcg cccccagcgg cccgccggtc atgccggagc agcacgactc
     3781 cgtcgttcac cccgacgagg ccccccgcct tgacgaagag ctccagcgtc tcggcgaggg
     3841 tgcgctcagg gccggagccg agggacgaaa ggagcgtctc ggcggcaggg gcggtgcaga
     3901 ccgcgtgaac gccggcccgg gccagagcct ccacgacgtc cgccatcgag gcacctgtgc
     3961 cgaggccctg cagtctcgtc tgcacgagca cgccgcggta tggctccagg cccgagtagc
     4021 cgacgaaatc gccgcgagca ggcgcttgcg ccgcaaagag cacggcagag agcacgagaa
     4081 ggagccggac acgcacggga tccacagagc agccctccag tgcactctcg gcgagccgag
     4141 tggttagtag acgccagcct gcaggatctg cccaggaagc ggcagcgagt tctcctggtt
     4201 gaggcaccag gtctcctccg gcacgccgtt gtagatggcc cagacgtgga aacactgctg
     4261 ccactcgaac ctcgtgcgcc aggtgacctg tatcagtggg ccgtactccg ggctgaggct
     4321 gaacgtcttc agaacgtcta tctcaggcgg tatggtaggg tcgctctgtt gggagtgaca
     4381 cccgtcctcg ttcctcaggt tctcgcactc ccagctccga tcgggctgga ttccctctac
     4441 gagtttggtg tggatctcgg tatcgtaggg atatgcaccc agggacctgt acagctgtga
     4501 cccgtatctc tcccaatcgt cgcagttgga ggtctcgaac gtgcagcaat ctgggtcgag
     4561 ggggtcggcc caagagcccc cagccaaccc ggccacgagc cccagaacga gcagggcggg
     4621 cgcggagcgc ctcaaagatc cgcaacgcaa agccatcact cccgttcggg tgtcggcggg
     4681 gtcggcggct cgaggccgcg acgctccgct ttcgtttgtc ctatccgtgc acccccgatg
     4741 gtgtcaacgc cgattgtggc ggcatcgcgc agcaggaggc agtagcgggc cggcggacgc
     4801 gcacccggtt gttgtccggc ctcgtcctcc acgagcatgt ggtctatcga ggcaacaaca
     4861 tcaagggccg gggtcgcccg atacctaccg gcctcgagcc ccgcccacta ctccgcctca
     4921 gcggtcgccc ccgcgttggc tggagcgcgc agtatcgaca gctggccctc tgcttcgcga
     4981 accgcctcgg ggctccacag gccggtgagg cccgcaccag gcctcgccag cagccagatc
     5041 aggctcggct tcccccgttg cctcgctacg aggtcctgga gtgcgagcgc cggctcagcg
     5101 agctccctta tgagcagccg cagccactcg gagggctcgc ctgacgctcc gtcggtgccc
     5161 gatggtctcg cgtcgccgac gcccaagtgc aggtgtgcgc caacgagcct tgggcgcgcc
     5221 caggtcgccg cacatgccgt ctcgagcact gcctccgggc tcgcgttccg gctgcagacg
     5281 tagacggcgc ggggctcgag gcgcgggtcg cacgtccact ccaccgcgtc ctgcctaaca
     5341 caccgcgaga cgacgtccgc gaagggcacc agccggcctg ctgcccaagg gccttcgggc
     5401 tcactgaagc tgagggccgc caccccgtgg aggggcgtcg cgagcttcgc ctggggcggc
     5461 gcgtccacta ccggaatgga gagcgactct agcggttcgg ccctgcccag gatcgcctcc
     5521 tcgaccgcag gctggggacc ggggccgagc cacactggcg aagggcccag gcccgccctt
     5581 cggaccagat cgttgtaggg cacgaaggct gtacccccgc cggccgtcag ggcgccgaga
     5641 cggtcgtcgg cctcgccgcg cacctcgagc cggaggcgga tggtaagacg cagcaccgcg
     5701 ccggccgcgg cgtcggcgct gctccagaaa gggctcggtc tgtcgtccgt ccgtgccagt
     5761 tcgatggcca cggccgtggc ctctggaccc aggtcgaatg ggcgcacgta gcatccgaga
     5821 aggactgcgc gccgcgttgc gggatcgagg agctccagaa gggccagcag caggttggtc
     5881 cgcctgatgc tcgagccgac gccgcgccgc tcgcgcgccc gggcctcccg cacaaagctc
     5941 actgcatagc gggagtctcg tgccacgatg gtcgccccag cgcaccgaag cgccgccaac
     6001 atccgcgtag tggagggagc atcgcgaccc tccagcaacg gcaacgcact ggtcctaccg
     6061 agatcctctt cggtgccgca tgccacaaca caatctgccg tctgtgccag cgccgccaga
     6121 atgtcgcgga cggtcaccgg gccctcggac ctcgccgcgc ccccgccgag gcacaagagc
     6181 gcgagccacg cgacgacgag ccgcgaatac gcgatggtgc ccatacggcc ggcccgcaca
     6241 ggcgtgccga ctgggcacgc tcgccaccac aagactccag gcgcgccgcg acaccgcact
     6301 cgcaccgcct ccttccactc ccgctacgcc tcacacactg actccgaacg cgccgcctcc
     6361 gtcgtgcgca gcgtctccgc
//
LOCUS       DAOSPL010000050         6300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_12483_length_6300_cov_3.441633, whole genome shotgun sequence.
ACCESSION   DAOSPL010000050 DAOSPL010000000
VERSION     DAOSPL010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12483_length_6300_cov_3.441633"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PLQ54_01640"
     CDS             complement(<1..369)
                     /locus_tag="PLQ54_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91993.1"
                     /translation="MGSNPTLSALSPRFLATLTEGLGRAPGKPRSRRERRARGERVRL
                     FIDIHCHAYRKPPPGYSFQTAEQVLERYDAEGIEKGCLLPIVSPEIYLPQTNEDILDM
                     AAQYPDRFIPCGMLPLFDVER"
     gene            complement(345..435)
                     /locus_tag="PLQ54_01645"
     tRNA            complement(345..435)
                     /locus_tag="PLQ54_01645"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(398..400),aa:Ser,seq:gga)
     gene            complement(510..601)
                     /locus_tag="PLQ54_01650"
     tRNA            complement(510..601)
                     /locus_tag="PLQ54_01650"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(564..566),aa:Ser,seq:cga)
     gene            complement(617..1126)
                     /gene="tadA"
                     /locus_tag="PLQ54_01655"
     CDS             complement(617..1126)
                     /gene="tadA"
                     /locus_tag="PLQ54_01655"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOS91994.1"
                     /translation="MAQTFEAGPCGASEDPAHSEALMALALAEAEAAPAHGDVPIGAI
                     VVHEGEVIARAHNRRDVDADPTAHAEILALRAAAKALGTWYLTGCELYVTLEPCAMCA
                     GALVLARVRSLTFGAPDPKAGACGSVFNLVQSPRLNHRLEVSGGVLGERCAEVLSAFF
                     EELRASKAN"
     gene            complement(1152..1226)
                     /locus_tag="PLQ54_01660"
     tRNA            complement(1152..1226)
                     /locus_tag="PLQ54_01660"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1189..1191),aa:Arg,seq:cct)
     gene            complement(1235..1307)
                     /locus_tag="PLQ54_01665"
     tRNA            complement(1235..1307)
                     /locus_tag="PLQ54_01665"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1272..1274),aa:Arg,seq:ccg)
     gene            1375..2571
                     /locus_tag="PLQ54_01670"
     CDS             1375..2571
                     /locus_tag="PLQ54_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOS91995.1"
                     /translation="MSSPPRPCAYSRARCRIIDPIGLLRDLIAIPSINPRDELSFARP
                     FGETAVIDHLEDVLARSRFDVERQTACPGRDNLLARVDGVDPSRVLLLESHVDTVGVE
                     GYRGDPFDPVLRDGRVYGRGSCDDKAQVAAMVAALADVTSSGRPQHTVLLVLAVGEEY
                     NFTGARALVESGLGADGAVVGEPTELQVITAHKGAVRMRCITEGVAAHSSEPSAGVNA
                     IYRMARVLAALERHAGALVGRQPHARLGTPTLSVGTIAGGRDTNIVPDRCVISLDRRV
                     LPGEDSGTVIAEIQRAVLDDPGLDFPVEFVVDLADHPMETPEGATITRSLCAACEAVR
                     GHSQVSAVRYGTDASKFAAAGIPSVVFGAGSIRQAHTVDEYVEVEQVWQATAVLRRLL
                     EQGLLG"
     gene            2594..4198
                     /locus_tag="PLQ54_01675"
     CDS             2594..4198
                     /locus_tag="PLQ54_01675"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HOS91996.1"
                     /translation="MEFDAILRSGDVCDGLGGTVRKADVGLHRGRITAMGDLSQARAA
                     CELSAAGLMVAPGFIDIHGHSDPGVLAGPRCESKVHQGVTTELCGMCGYSPAPVSEPC
                     REDLRAGAFFPEAEFEWDWLSMAEYLDRVQAARPSMNIVQAVGHTPLRASVMGYEARP
                     ATPEELDTMGRMLAQALEEGGAGFSSGLIYPPGSFAPPEELEFLCRVAARFGRGYYTH
                     MRSEGDGLIDSVCETIAAARVAGVPVEIAHHKAAGRDNWGKVHDSMALIERARDEGVD
                     VRVDVYPYTAGSTTMTSLLPDWAHEGGREALLGRLRDPATRRRLADEIQGPRGFAGPP
                     WGEEAWTRVLVAAVADQRDREFEGLTLAQLGQVCAKPPVEAMFDLLLAHETRVSIVSF
                     TMCDDDMAFVLGHTLSTVGSDASAVSALNGQRHGKPHPRAYGTFPRVLGRFARDRKLM
                     PLPEAIRKMTSEPARRIGLRDRGVLREGAAADVVVFDPATIMDRATYADPHQFPAGIR
                     QVWVNGVLVVHEGHHTGAGPGQVLRL"
     gene            4252..5250
                     /locus_tag="PLQ54_01680"
     CDS             4252..5250
                     /locus_tag="PLQ54_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOS91997.1"
                     /translation="MAPWTRDSILGLGRQFTQSRVLLTAAELDVFTPLAREPLTLEEV
                     AARVGGDLRGTRILLDALAGMGLIVKANAHYRCPAEVAAALSADSPTSVRPMLMHSNA
                     LWGRWSRLTDIVRGEKPSGNPLTAAYRPEEREAFIGAMHVVSQGRAPEVVDAVGAGRA
                     RNLIDVGGASGTYTEAFLRACPEMRATLFDLPPVIEMARRRLEPTGLLPRICLVAGDF
                     LVDPMPGGHDLAFVSAIIHQNSPADNETLYRNCFRALEPGGRIVIRDHILDETRTQPP
                     AGALFAVNMLTATDGGDCYTFAEIRQGLEQAGFVRVRQIAEDEQMSGLVEAFKPRE"
     gene            complement(5335..5574)
                     /locus_tag="PLQ54_01685"
     CDS             complement(5335..5574)
                     /locus_tag="PLQ54_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS91998.1"
                     /translation="MQLSAVARRIVAALTLTALIALAAPGCKSGETRSGTDMSPPPAK
                     AKMMGGTPVGKEFVQKQGDQQAPPMKTKTTETTNP"
     gene            complement(5607..>6300)
                     /locus_tag="PLQ54_01690"
     CDS             complement(5607..>6300)
                     /locus_tag="PLQ54_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOS91999.1"
                     /translation="RGFTLIELLVVIAIIAILAAILFPVFAKAREKARTTACLSNCKQ
                     IGLGVMMYCQDYDEKYPLKRYGNGDPSTACNDGTRSGTWRTVTQPYVKNWQLYRCPSS
                     TCNGGNPEEIRLGALATPVYTAALNFSYHYNGSVMCGTSPKGMADFPSPAQQVIIHEN
                     RICPPDAGGWCNTAFSYAPHMGGKNWVFADGHAKWIRPSQICSPLNMWVATDPPMSAY
                     DYTCSDSWTYPQ"
BASE COUNT          973 a         2076 c         2138 g         1113 t
ORIGIN      
        1 gcgctcgacg tcgaacagcg ggagcatgcc gcacgggatg aaccggtcgg ggtactgcgc
       61 ggccatgtcg aggatgtcct cgttggtctg cgggaggtag atctcggggc tcacgatggg
      121 caacaggcag cccttctcaa tcccctcggc gtcgtaccgt tcgagtacct gctccgccgt
      181 ctgaaacgag tagccgggtg ggggcttgcg gtaggcatga cagtgtatgt cgatgaacag
      241 acggaccctc tcccctcggg cgcgccgctc gcggcgtgag cgaggtttcc ctggagctcg
      301 gccgagcccc tcggtgaggg ttgccaggaa tcgcggggaa agggcggaga gggtgggatt
      361 cgaacccacg aggcgactgc tagccgccta cgcgctctcc aggcgcgcgc cttcgaccaa
      421 ctcggccacc tctccgcaac gtggagacca ccggcatcgt tgcgggtttc gagctctcag
      481 actatggctt gccccccgat gggaaagggc ggagagggtg ggattcgaac ccacgggcga
      541 ggtctgaccc ccgccactcg atttcgagtc gagcgctttc gaccaactca gccacctctc
      601 cgcgccaacg ggaccatcag ttcgccttgc tggcacggag ctcctcgaag aatgcgctca
      661 gaacctcagc gcatcgctct ccgagaacgc ctcccgacac ctccagccga tggttcagcc
      721 tgggggactg caccaggttg aacaccgaac cacacgcgcc cgccttcgga tccggcgccc
      781 caaaggtcag actccgcacc cgggccaaca ccaaagcgcc cgcacacatg gcgcagggct
      841 ccagcgtcac atagagctca caacccgtca ggtaccaagt gcccagagcc ttggccgccg
      901 cgcggagcgc gaggatctcg gcatgggccg tcggatcggc gtcgacatcg cgccgattgt
      961 gtgcccgggc aatcacctcg ccctcatgga ctacgatggc tccgataggc acatccccat
     1021 gcgccggcgc ggcttcggcc tccgccagcg ccagggccat gagggcttcg gaatgagcgg
     1081 gatcctcgct cgcgccacac gggcccgctt cgaacgtctg ggccatacgg ttcatcacgg
     1141 ctcgctttgg gcggccccgg cccgattcga acgggcggcc ttgaacttag gaggttcctg
     1201 ctctatcctg gctgagctac ggggccaagc tcggcgcgcc cggcaagagt cgaacttgcg
     1261 acacccggct ccggaggccg gtgctctatc ctctgagcta cgggcgcacg atgtcgcttt
     1321 ggcaacagac gagtttaggc cctccggggg ccacttgtca aaccgcggcc gcgtgtgtcg
     1381 agcccaccgc ggccctgcgc ctactcgcga gcgaggtgcc ggatcatcga ccccatcggc
     1441 ttgctgcgtg acctcattgc catccccagc atcaatcctc gcgatgagtt gagcttcgcc
     1501 cgtccgtttg gtgagacagc agtcatcgac cacctggagg acgtgctcgc ccgctcgcgc
     1561 tttgacgtcg aacgtcagac cgcgtgcccg ggccgcgaca accttctcgc ccgcgtcgat
     1621 ggtgtcgatc cctcccgtgt cctcctactc gaatcccatg tcgataccgt gggtgtcgag
     1681 ggctatcgcg gcgatccctt cgaccctgtg ctccgcgacg gccgcgtcta tggccgcggg
     1741 tcgtgtgacg acaaggccca ggtggcggcg atggtggccg ccttggccga tgtcacctca
     1801 agcgggcgcc ctcagcacac cgtgctcctg gttctcgcgg tcggagagga gtacaacttc
     1861 accggtgctc gtgctctggt cgagagtggc ctgggggccg acggcgctgt cgtgggcgag
     1921 cccaccgagc tccaggtcat caccgcccac aagggggccg tgcgcatgcg gtgcatcacg
     1981 gagggcgttg ccgcgcacag ctctgagccg tcggccggcg tcaacgccat ctaccgcatg
     2041 gcgcgggttc tggcagcgct ggagcgccac gcgggggctc tggtgggccg ccagccccat
     2101 gcgcgactgg ggacgccgac cctgagcgtg ggcaccatcg ccggtggacg cgacacgaac
     2161 atcgttcccg atcggtgtgt catcagcctc gaccggcgcg tgctaccggg cgaggactcc
     2221 ggcaccgtca tcgctgagat ccagcgtgcc gttctggacg atcccggcct ggacttcccg
     2281 gtggagttcg tggtagacct ggccgaccat cccatggaga ccccagaagg cgccacgatc
     2341 acgcgaagcc tctgcgccgc ttgcgaagcg gtacggggcc acagccaggt gtcggctgtg
     2401 cgttacggga cggacgccag caagttcgcc gccgccggca ttccctcggt ggtgtttggt
     2461 gccggcagca ttcgccaggc gcacacggtc gatgagtatg tcgaggtcga gcaggtctgg
     2521 caagctaccg cggtgcttcg gcggctgctc gagcaggggc tcttgggcta gcggaagagg
     2581 gcatgggcgt acgatggagt tcgacgcgat cctgcggtcc ggagacgtct gcgatggcct
     2641 gggtggcacg gtgcggaaag ccgatgtggg cctccatcga ggccggatca ccgccatggg
     2701 cgacctgagc caggctcgag cggcttgcga gctcagtgcc gccggcctta tggtggcacc
     2761 cgggttcatc gacattcacg gacactcaga ccccggggtg ctcgcaggcc cccggtgcga
     2821 gagcaaggtt caccagggcg tcaccaccga gctgtgcggc atgtgcggct atagcccagc
     2881 accggtgagt gagccctgcc gcgaagacct gagggcgggc gcgttcttcc ctgaggccga
     2941 gttcgaatgg gactggctct cgatggctga gtatctggac cgggttcagg ccgctcggcc
     3001 gtccatgaac atcgtccagg cggtgggcca tacacccctt cgtgcctccg tcatgggcta
     3061 tgaggcgcgc ccggccacgc ccgaagagct cgacaccatg ggccgcatgt tggcgcaggc
     3121 tctggaggaa gggggggccg gtttctcctc ggggctgatc tacccgcccg gttccttcgc
     3181 tcctccggag gaactcgagt tcctctgtcg ggtggcggcc cgtttcggcc ggggctacta
     3241 cacccacatg cgcagcgagg gcgatggcct gatcgattcg gtgtgcgaaa ccatcgcggc
     3301 ggctcgagtc gccggggtcc cggtggagat cgctcaccac aaagcggccg gacgggacaa
     3361 ctggggcaag gtgcatgact ccatggccct catcgagcgg gctcgcgacg agggcgttga
     3421 cgtacgggtc gacgtctacc cctatacggc cggaagcacg accatgacct cgttgctgcc
     3481 cgattgggct cacgaggggg gccgcgaggc gcttctcggc cggctgcgcg atcccgcgac
     3541 acgccgccgt cttgccgatg agatccaagg accccggggc ttcgccggcc cgccttgggg
     3601 cgaggaggcg tggacccgtg tgctcgtcgc cgccgtggcg gatcagcggg atcgcgagtt
     3661 cgagggcctc acactcgccc agctgggcca ggtgtgcgcc aagccgcccg tcgaggccat
     3721 gttcgacctg ctgctcgccc acgagacccg cgtgtcgatc gtcagtttca ccatgtgcga
     3781 cgacgacatg gccttcgtcc tcggacacac gttgtcgacg gttgggtcgg atgcgtccgc
     3841 cgtgtccgcg ctgaacggtc agcgccatgg caaaccacac ccgcgcgcct atggcacgtt
     3901 cccccgtgtg ttgggtcggt tcgcccgcga tcgaaagctc atgcctctgc cggaggcgat
     3961 ccggaagatg acctccgagc ccgcccggcg cattggactg agggaccgcg gagtcctcag
     4021 ggagggagca gcggccgatg tggtcgtctt cgatccggcg accatcatgg accgggccac
     4081 ctatgccgac ccgcatcagt tccctgccgg gatccgccag gtttgggtca acggggtgct
     4141 tgtggtccat gagggccacc acacgggggc cgggccaggc caggtgttgc ggctctagcg
     4201 gtccttgcct tggggccggc aggcccagtg acgtgagagg ggttggcgag catggcgcct
     4261 tggacccgcg acagtatcct ggggctgggc cggcagttca cgcagtcgcg agtgctgctc
     4321 accgccgccg agctcgatgt gttcactcca ctggcgaggg agccgctgac gctggaggaa
     4381 gtggcggccc gggtaggtgg tgaccttcgc ggcactcgca tcttgctcga cgcgctggcg
     4441 ggtatgggcc tgattgtgaa ggcgaacgcg cactaccggt gcccggccga ggttgcggcg
     4501 gccttgtctg ccgattcgcc gacttcggtg cggcccatgc tcatgcattc gaacgcgctc
     4561 tgggggcggt ggtctcgcct gaccgacatc gtccgggggg agaagccctc aggcaacccg
     4621 ctcaccgcgg cctaccgtcc ggaggagcgc gaggccttca tcggggccat gcacgtcgtc
     4681 agccagggcc gagcccctga ggtggtggat gctgtcggcg cgggccgcgc ccggaacctg
     4741 attgacgtcg gcggggcctc ggggacctac accgaggcgt tcctccgggc ttgccccgag
     4801 atgcgtgcga ctctctttga cctgcccccg gtgatcgaga tggcccgcag acgtcttgag
     4861 cccaccgggc tgctcccgag gatctgtctg gtggcaggcg acttcctggt cgatccgatg
     4921 cctggcgggc acgatctggc gttcgtgtcg gccatcatcc accagaacag cccagcggac
     4981 aacgagacgc tctaccgcaa ctgcttccgt gccctggagc cgggcggccg cattgtcatc
     5041 cgtgatcaca ttctggacga gacgcggacc caaccgccgg cgggcgcgct gttcgccgtg
     5101 aacatgctca cggccaccga cgggggcgac tgctacacct tcgccgagat ccgtcagggc
     5161 ctcgagcagg ccggtttcgt gcgcgtcaga cagatcgccg aggacgagca gatgagcggc
     5221 ctggtggaag ctttcaagcc gcgggagtag aagcgcacgc gcggcatagc gccgggccac
     5281 tccgtctcgg agtggcccgg ctcggtctac ggtcttggtc tgtcgaggcg gagtctacgg
     5341 gttagtggtc tccgtcgtct tcgtcttcat cggcggtgcc tgctggtctc cctgcttctg
     5401 cacgaactcc ttgccgaccg gcgtgccacc catcatcttg gccttggccg gaggcgggga
     5461 catgtcggtg cccgagcggg tctcacccga cttgcagcct ggagcggcga gcgcgatcag
     5521 ggccgtaagg gtgagggccg ctacgattcg tctggctacg gcgctcaact gcattccgtg
     5581 ctcatctcct gttcgtccac cgagttctac tgcgggtagg tccagctatc gctgcaggtg
     5641 tagtcatacg cgctcatggg aggatcggtc gcaacccaca tgttcagggg cgagcagatc
     5701 tgtgacggcc ggatccactt ggcgtggccg tcggcgaaaa cccagttctt gccccccatg
     5761 tgcggtgcat agctgaacgc cgtgttgcac cagccgcccg cgtccggtgg gcagatgcgg
     5821 ttctcatgga tgatgacctg ctgggcgggc gatgggaagt cggccatgcc cttgggcgag
     5881 gtgccacaca tcacggaccc gttgtagtgg tagctgaagt tgagtgcggc cgtgtagacc
     5941 ggcgtcgcca gggcaccgag ccggatctcc tcaggattac ccccattgca ggtactgctc
     6001 ggacagcggt agagctgcca gttcttgaca tagggctggg ttacggtccg ccaggtcccg
     6061 ctgcgtgtgc cgtcattaca ggccgtactg gggtcaccgt tgccgtaccg tttgagcggg
     6121 tacttctcgt cgtagtcctg gcagtacatc atcacgccga ggccgatctg cttgcagttg
     6181 gacaggcacg cggtggtccg tgccttctcc cgtgccttgg cgaagaccgg gaacaggatc
     6241 gccgctagaa tcgcgatgat cgcaatgacc acaagaagtt cgatcagggt gaaccctctc
//
LOCUS       DAOSPL010000051         6269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_12558_length_6269_cov_3.434986, whole genome shotgun sequence.
ACCESSION   DAOSPL010000051 DAOSPL010000000
VERSION     DAOSPL010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12558_length_6269_cov_3.434986"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PLQ54_01695"
     CDS             complement(<1..573)
                     /locus_tag="PLQ54_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOS92000.1"
                     /translation="MTQPATEAMPRDLTVALAGNPNSGKTSLFNELTGSRQHVGNWPG
                     VTVERKEGYRRTASGRARVIDLPGTYSLTASSPDERVARDFIISGESDVVIAVVDASN
                     LERNLYLVVQLIELGTPLVVALNMSDVAEDRGFVINVDKLSELLDVPVIPTCAHVGEG
                     VEPLWEAALAQAREARAPRPIRYGRQIDEAI"
     gene            complement(575..802)
                     /locus_tag="PLQ54_01700"
     CDS             complement(575..802)
                     /locus_tag="PLQ54_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOS92001.1"
                     /translation="MPLTALAPGQRAFVTAISGGHGMRRRLASMGLTTGCEVVALISR
                     GQGPVVVAVRQTRLALGRGIASRIDVEPLEG"
     gene            complement(805..1317)
                     /locus_tag="PLQ54_01705"
     CDS             complement(805..1317)
                     /locus_tag="PLQ54_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOS92002.1"
                     /translation="MGAESLQLTATMEDYLESILLLSRKRRVVRMRDIANLLGVTPST
                     ATGAVQALKKHGLLSHEKYEDVILTPRGQSIAEDVYQRHEDIRDFLQAVLLLDREMAE
                     EEACRIEHSITPFTLERLRRFAATLRLCNQGEPGCLRAFRTYVDTGELVPPPCAQVAG
                     GQRLQPEEVG"
     gene            1561..3585
                     /locus_tag="PLQ54_01710"
     CDS             1561..3585
                     /locus_tag="PLQ54_01710"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOS92003.1"
                     /translation="MTYGSQLRRLILAVLVASGPLLCSPNAVAQAEPPWKTPGHAFFG
                     NPGGYARDLYNQYTASRAELARLKASLASTQSQLEGYQGTGFIGGLAIAQNDADTIIR
                     KEALKRAIAAEEQKQQELVREWDQWCQFGLLLGSLTDANETIEDVYLDRSVFPPAQKK
                     CRIDRVAYRLKYFLEGKTAPGGAGAGGGGGGQAFPQEPFNSLQITYSISGAALGTPSD
                     GGPFEGWVRTYEGSLQGDVLRVSGTVTKQCHEGWPGDAYIEVSVGDQKAEQSVHLDSK
                     AEATVTRHYDVSVPIPPNATGGSINVMLGAVYQNGERRFVQVIGSFQGRTTPTGVPET
                     APREPTADKPTATAAGRLYVLAKPAQLVPAGSAEAGPVDIPTGIPLGWQPGDRISTAL
                     DSPIAIVIPDSVRLVLSGGAKAVLLDGGLSLGTGSAVIDLLPRESAFEVRTPAATVSS
                     TGATVAVQAQDDGTTLVGVARGRVRVTDRASGAQAAVGPGQGLSASPDVRLAEPRALT
                     DDQIRSLFAGEVALLEAPMPPPGDVLGETAAPSAGPGGAAPATPTTPPTPSGPAPATG
                     TPDDIGGLSVGTDVADNQVVGAADRFAKPTRVATVYEFADVPGDTPCEIVWTRNGKEI
                     LKDSRPIGGSGSVSFDISMVGDKPLPPGTYTVTITVGGKIAARKTFTVTD"
     gene            3751..4764
                     /locus_tag="PLQ54_01715"
     CDS             3751..4764
                     /locus_tag="PLQ54_01715"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOS92004.1"
                     /translation="MRAMAASGVFVLLWAFAAGCGSDATADLSDPGPDPALTAVRGLS
                     RLVTADEVSGSAGACTGEVRSGTGRPVAQATVELKRANAPQYDGVTVQTNANGQFGFD
                     AVPPGQFTISVQDGRAVRTQALVIEAGQVTRVDVGAPATPPMGEPGLGVLEPYLPDPG
                     DLAAFQRTAVDGAGSPVDVRIVYTAIDASAGRIEFTRTIDGVEESVTQCYRSEGGAVY
                     LTEETVGDTVTSYSPGLPIFSSGDEGDVVATNVDVSSGGTTTSHRAVLTVLPTRTVTV
                     QGVELSATGCEVYVTELGVAAPDVTITRTWYVPSCGAVRSERTPPSGSGVVEELVTYT
                     AAP"
     gene            4761..6044
                     /locus_tag="PLQ54_01720"
     CDS             4761..6044
                     /locus_tag="PLQ54_01720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS92005.1"
                     /translation="MKPGQPDCHTARWRGITLGALLVGYGGYYLCRSDLSLATPLLLQ
                     EFGGEGFTKTHIGAIVSVGLAVYALGSIVNGIGADLFGGRRLFLIGAAASAVCTVAFG
                     ASSGLMAFGALWALNGYFQSMGWVGLIGVAGRWFPAANQGTVMGLISISYLAGDAIVR
                     LGLGLVLSHGVGWRGMFFVAAGTLAALTCANTAVLKARPADVGATDPDSRAGPVEASP
                     ARSRRALRSLLHNPTLWLLCGMSFGMTLVRQTFNQWTPTLLWETTGLNVGTAALSSLL
                     FPLVGALAAVTAGVVTDRAGWRPGPLVASSLTVLVVALGALCLPAVQGDARVVLALTS
                     AVAFFLMVPGSLCAGIVAMRLGGREAGATAAGIINSAGYMGAVLSGLGIGSVAQQCGW
                     AAAFGALTAVAAGSAVLGVLFWARERREIHRGPRG"
     gene            complement(6070..>6269)
                     /locus_tag="PLQ54_01725"
     CDS             complement(6070..>6269)
                     /locus_tag="PLQ54_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92006.1"
                     /translation="LTRRLADEDYTVREAARESLARLQGTEEALAGRVRLLHLEPDRW
                     AGSATRRDLPKPGGAYRDPLG"
BASE COUNT          958 a         2247 c         2057 g         1007 t
ORIGIN      
        1 gatggcctcg tcgatctggc ggccgtagcg gatgggccgc ggcgcccggg cctctcgcgc
       61 ctgcgccagt gccgcctccc agagcggttc cacaccctcc cccacgtgtg cgcaggtggg
      121 aatcacggga acgtccagca gctcggagag cttgtccacg ttgatcacga aaccccggtc
      181 ctcggccaca tcactcatgt tcagcgccac gaccaggggg gtgcccagtt cgatgagctg
      241 cacgaccagg tacaggttgc gctcgaggtt gctcgcatcc accaccgcga tgaccacatc
      301 actctcaccg ctgatgatga aatcgcgagc cacgcgctca tcgggcgagc tcgctgtcag
      361 ggagtaagta cccggaagat caatgactcg ggcgcggcca ctggcggtcc ggcggtagcc
      421 ttctttccgc tccacggtga cccccggcca gttcccgacg tgctgacgcg atcccgtgag
      481 ttcgttgaac agggacgtct tgcccgagtt cgggttgccg gcgagtgcga ccgtaagatc
      541 acgcggcatg gcttcggtgg cgggctgtgt cacttcaccc ctccagcggc tccacgtcga
      601 tcctcgaagc gatgccccgg cccaacgcca gccgcgtctg ccgcaccgcc accaccaccg
      661 ggccctggcc tcggctgatc agcgccacaa cctcgcaccc cgtggtcagc cccatgctcg
      721 ccaagcgtct gcgcatgccg tggccgccgc tgatcgccgt cacgaaagca cgctggccgg
      781 gcgcaagtgc cgtcagaggc atatctagcc aacctcctca ggctgcagcc gctgtccccc
      841 ggccacctgg gcacacgggg gaggcaccag ctcgccagta tccacgtagg tacggaacgc
      901 ccgaaggcac ccgggctccc cctggttgca gagcctcaga gtcgctgcga agcgcctcag
      961 acgctccaat gtaaacggtg tgatactgtg ctcgatccga cacgcctcct cctcggccat
     1021 ctcccgatcc agaaggagta ccgcctggag aaaatcccgg atgtcctcgt gacgctgata
     1081 cacgtcctca gcgatcgact gcccgcgcgg cgtcagaatg acgtcctcat acttctcatg
     1141 tgagagcaga ccatgcttct tcagagcctg cacggcaccc gttgctgttg agggagtcac
     1201 cccaaggaga ttggcgatgt cgcgcatccg caccacgcgg cgcttcctcg agagcaacaa
     1261 gatgctctcc agatagtcct ccatcgtggc cgtcaactgc aggctctccg cgcccatggg
     1321 ctctcccccg atccgggctg tgacacgccg ccaaggcccc gaaccgtacc gcccgagacc
     1381 ctaccgatcg tgatagccca gaagagtgcc catgtcaaaa caatctttgg cggaccgaaa
     1441 agcaccggcg cttccgtggg ccggggcgct ccgacgcccg cgaggagcca agcctgcaag
     1501 cagagaacat ccgggcgtgc cattcctcgc gctcggcaac ccgggaggag tgagtgtggg
     1561 atgacgtacg ggagccagct ccgccgactg attctggctg tgctcgtcgc ttcggggccg
     1621 ctgctgtgct cgcccaacgc ggtggcccaa gccgagccgc catggaagac gccgggccac
     1681 gccttcttcg ggaacccggg cggatatgcg cgggacctct acaaccagta cacggcgagc
     1741 cgggccgagt tggcgcgtct caaagccagc cttgcgtcga cccagagcca gctcgaggga
     1801 taccagggca ccgggttcat agggggcctg gccatcgccc agaacgacgc cgacaccatc
     1861 atccgcaagg aggcgctcaa gcgcgccatc gccgccgagg agcagaagca acaggagctg
     1921 gtgcgcgaat gggatcagtg gtgccagttc gggctgctcc tcggttcgct caccgatgcc
     1981 aacgagacca tcgaggatgt ctacctggat cgtagcgtct tccctccggc ccagaagaag
     2041 tgccggatcg accgcgtagc ctaccggctc aagtacttcc tcgagggcaa gaccgccccg
     2101 gggggcgccg gtgcgggtgg gggcggaggc ggccaggcgt tcccccagga gccgttcaac
     2161 tcgctccaga tcacctactc catctcgggc gccgcccttg ggactccctc cgatggcggg
     2221 cccttcgagg gatgggtgcg gacctatgag gggagcctgc agggcgatgt gctccgggtc
     2281 tctggaaccg taaccaagca gtgtcacgag ggctggcccg gcgatgcgta catcgaagta
     2341 tcggtcggag accagaaggc ggagcagtcg gtgcacctcg atagcaaggc ggaggccacc
     2401 gtcacgcggc attacgatgt atcggtgccc atccccccga acgccacggg tggcagtatc
     2461 aatgtgatgc tcggtgccgt ctaccagaac ggcgagcggc ggttcgtcca ggtcatcggc
     2521 tcgttccagg ggcggaccac acccaccggc gtgccggaaa cggcaccccg tgagccgacc
     2581 gcggacaagc ctaccgccac cgccgcaggt cgcctctatg tcctcgccaa gccagcccag
     2641 ctcgtgcccg caggttccgc cgaagcgggc cccgtcgaca taccgacggg cattcccctt
     2701 gggtggcaac ccggcgaccg catcagcacg gctctcgact cacccatcgc catcgtgatt
     2761 cccgacagcg tgcgactggt gttgagcggc ggcgccaagg ccgtcctgct ggatggcggg
     2821 ctgtcgctgg gcaccggcag cgccgtcatc gacctcctgc cccgcgaatc cgccttcgag
     2881 gtgcgcaccc ccgccgccac ggtctcgtcc accggtgcca ccgtcgcggt ccaggcccag
     2941 gatgacggca caaccctggt tggggttgcc cggggccggg tccgcgtcac ggaccgcgcc
     3001 agcggagccc aggcggctgt ggggccgggg caagggctga gcgcctcgcc cgatgtcaga
     3061 ctcgccgagc cgcgtgcgct cacggatgac cagatccgca gcctgttcgc cggcgaggtg
     3121 gccctgctcg aggcgcccat gccgcccccg ggcgacgtcc tgggcgaaac ggccgcgccg
     3181 tctgcgggcc caggcggagc cgcccccgcc acacccacca cgccccccac accgtccggc
     3241 cccgcaccgg caaccggcac gccagacgat atcggcggtc tgtcagtggg caccgatgtg
     3301 gccgacaacc aggtcgtcgg agccgcggac cggttcgcca agccgacccg cgtcgccacc
     3361 gtctacgagt tcgccgatgt gcccggggac actccatgcg agatcgtgtg gacgcgcaac
     3421 ggcaaggaaa tcctcaagga ctccagaccc atcggaggaa gcggctccgt gtccttcgac
     3481 atcagcatgg tcggtgacaa gcccctgccg cccggcacgt acacggtgac catcaccgtc
     3541 ggcgggaaga tagccgcccg caagacattc accgttacgg actagcgggc tgcccggagt
     3601 tggggccgtg ggagcgacgg ctggcctccc ccaccgtcgg ccatccgcca tcggccgggt
     3661 gtcctctagc ctcgaaccga tcgccctctg ccggccttgt ttccctggag cacccgaaac
     3721 ccgggtcacc aggagaggga gatcgttccg atgcgcgcca tggcggcctc tggtgtgttc
     3781 gtactgctct gggcgttcgc ggccggttgt gggtcggatg cgacggccga tctgagcgac
     3841 ccggggcccg atcccgcgct gactgccgtg cgcggactgt cgcgtctggt cacggcggat
     3901 gaggtgtccg gctccgccgg tgcctgtacc ggagaagtcc gcagcggcac cggcagaccc
     3961 gtggcgcagg ccacggtcga gctgaagcgc gccaacgcgc cccagtatga tggggtgacc
     4021 gtccagacca acgcgaacgg ccagttcggc ttcgatgcgg tcccgcccgg tcagttcacc
     4081 attagcgtcc aggatggtcg ggccgtgcgg acccaggccc tggtgatcga ggcgggccag
     4141 gtgacgcgag tcgacgtcgg tgcccccgct acaccgccga tgggcgagcc cgggttgggt
     4201 gtgctcgagc cctaccttcc cgatcccggc gacctcgccg ccttccagcg caccgctgtg
     4261 gacggggcgg gctccccggt cgacgtgagg atcgtctaca ccgccatcga tgcgtccgct
     4321 ggccgcatcg agttcacgcg gaccatcgac ggggtcgagg agtccgtgac ccagtgctac
     4381 cgctccgagg gcggcgccgt ctatctaact gaggagaccg tcggcgacac cgtcacttcc
     4441 tacagccccg gcctgcccat cttctcgtcg ggcgacgagg gagacgttgt ggcgaccaac
     4501 gtggacgtgt cgtcgggcgg tacgaccaca tctcatcggg ccgtgctgac cgtgctaccc
     4561 acgcgtacgg tgacggtcca gggcgtcgag ctgtcggcca cgggatgtga ggtgtacgtg
     4621 accgaactgg gggtcgccgc tcccgatgtg accatcaccc gcacgtggta cgtgccgtcc
     4681 tgcggtgccg tgagatcgga gcgtacgccc ccaagcggct cgggtgtggt cgaagagctc
     4741 gtcacctaca cggcagcccc gtgaagccgg gccagcctga ctgccacacg gcccgctggc
     4801 gcgggatcac actgggcgcg ctcctggtcg gctatggcgg ctactacctc tgccggtcag
     4861 acctgtcgct tgccacccca ttgctcctcc aggagtttgg cggcgagggt ttcaccaaga
     4921 cgcacatcgg tgccatcgtc tcggtggggc tggcggtcta tgccctgggg agcatcgtca
     4981 acggcattgg cgccgacctg ttcggcggcc gtcggctgtt cctcatcggc gcagccgcct
     5041 ccgccgtctg caccgtggcc ttcggcgcgt catccggcct gatggccttc ggggctctct
     5101 gggcactgaa cggttacttc cagtccatgg gttgggtggg cctcatcgga gtcgctggcc
     5161 gctggttccc tgccgccaac cagggcacgg tcatgggcct gatctccatc agctacctcg
     5221 cgggcgatgc catcgtccgc ttgggcttgg ggctcgtgct gagccatgga gtcggatggc
     5281 gcgggatgtt cttcgtcgcc gccggcaccc ttgcagcgct gacctgcgcc aacaccgccg
     5341 tgctcaaagc ccggccggcc gatgtgggcg caacagaccc ggacagccgg gcgggacctg
     5401 tcgaagcctc acccgcccgg tcgcggaggg cgctccggtc gcttctccat aaccccacgc
     5461 tgtggctcct gtgtggcatg agctttggca tgaccctggt tcgtcagacc ttcaaccagt
     5521 ggacaccgac gctcctgtgg gagaccacag ggctcaacgt aggcacggcg gccctctcga
     5581 gcctcctgtt ccccctcgtc ggcgctctgg cagccgtcac ggccggcgtg gtcacagacc
     5641 gtgccggctg gcgcccgggc cccctcgtgg ccagcagcct caccgtcctg gtggtcgccc
     5701 tcggggccct gtgccttccg gccgtccaag gcgacgcccg cgtggtgctc gcgctgacct
     5761 cggccgtggc cttcttcttg atggtgcccg gttcgctctg cgccggcatc gtggccatgc
     5821 gcttgggcgg acgcgaggcc ggcgccaccg cggcgggcat catcaactcg gcgggctaca
     5881 tgggcgccgt gttgtcgggg ctcggcattg ggtccgtggc ccagcagtgt gggtgggcag
     5941 ccgccttcgg cgccctgacg gccgtggccg ccggcagcgc cgtgctcggc gtgctgttct
     6001 gggcccgcga acgccgcgag atccatcgag gcccacgggg ctagcttgcc ggcgcgacgg
     6061 cctgacgccc taccccagag ggtctcggta cgcgccccct ggcttcggta ggtcccgtcg
     6121 cgtggccgac cccgcccacc ggtccggctc gagatggagc aggcgcacgc gaccggccaa
     6181 tgcctcttcc gtcccctgca gccgcgccag cgattcgcgc gcggcttcgc gcaccgtgta
     6241 gtcctcgtcg gcgagccgcc gcgtcagcg
//
LOCUS       DAOSPL010000052         6262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_12578_length_6262_cov_3.564846, whole genome shotgun sequence.
ACCESSION   DAOSPL010000052 DAOSPL010000000
VERSION     DAOSPL010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12578_length_6262_cov_3.564846"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PLQ54_01730"
     CDS             complement(<1..279)
                     /locus_tag="PLQ54_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92007.1"
                     /translation="MRLAPLILAALALAGPAAVRAAQEATPAFAFTPEPSECEGEYAV
                     RCTVASAKWPADAEARIAFDIHDDGSFHTLRISGKACRWGASVPIETST"
     gene            complement(276..1508)
                     /locus_tag="PLQ54_01735"
     CDS             complement(276..1508)
                     /locus_tag="PLQ54_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92008.1"
                     /translation="MRPTASRPFLPGLVAVAVVCGIACAQPPEIPPAPAPHALRADGF
                     LRHWLVLSPLPAPGGLAAPGLRDEAQVRPAPGDTVNVGGQELTWTRYACRPDDPTVRL
                     SPGVDSFIGYAVTYIVCDVEFKGLQLLCGADAGVRIWLNGGEILNAPGAMGPDGHKVV
                     GVSLRKGTNPLVLKVLGGGGFSCTVRFADETGAPIPDLFLSLLPQPARWPLAKAGAGG
                     ATGVPTDDGYIRDWVTLTHFPSNGSLDTYIVDDEANLRPRPGDRVTHGDQQHGWAPHR
                     TVNFLLDLVHVVGGPCDGALGYGVAYVLCNDTMSNLTLCVGADDIARIWLNGELMMTT
                     QSGWLAPDRDTRGGVTLRRGLNVLVGKVLNNDHLWQLSVRFLDAAGDPVTDFSLAYSP
                     DEPVLRPVQSWVWAGGAR"
     gene            1852..2178
                     /locus_tag="PLQ54_01740"
     CDS             1852..2178
                     /locus_tag="PLQ54_01740"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS92009.1"
                     /translation="MRRRKGFTLIEMMIVILVIAVLATLVGVSVRNARERSRQATLTA
                     QLNIMHNAIMYYQNDMSSWPPDLSALTSTTNSDPNYHGPYLERVPPHPYHGTWTYDSS
                     TGRVTP"
     gene            2343..4373
                     /locus_tag="PLQ54_01745"
     CDS             2343..4373
                     /locus_tag="PLQ54_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92010.1"
                     /translation="MSEPALPPAGPGQGVHPGAGSETNVGATFRVCLLALLIAPLNAI
                     WVVQMDGVRSLGMPTLAAPFFNVVFIISVAVAVNGAMRRLLPRFALSHAELVVLYILM
                     SILTSGVGYDTVNFTFATATGAYRYGTPESGWREAFFDYLPSGMLVEDADTLDRLWAG
                     GAGDPAHRWSWSNLAGPWARPECWRGVIGPMVRWWVFLSALWLAPMGLAVIFRYRWIE
                     AERLTFPITYLPLEISRPDAWMLRHRYMWAGFGLAAGISLLNGLHVLYPTVPQIPIRL
                     EQVPILNLGNQLTDRPWNAVGWFWLCFYPFVIGIGLLLPLELPLSCALFYVFFKAQLV
                     FFAWLGMTQKPEFPYQKEQSYGGYVALLLFSLVAGRQYYIRVAKQVIGLVHMPTDRSE
                     PMSYRAATALFVAGAAYVVLVGVQQHMAWWVSGLFFAQYFLLTAIIGRIRAETGIPSH
                     EIERLGPTVMLGNILGARILGPRNLTGASVFFGFTRGMRNIPFPHQAEGLKFAERLGM
                     PSRRLMAVMMAAVPVGLAVGIWAHYDQVFRYGYAATWRPVHGWACREAWDQLVAWINT
                     PAGFHSGRATACALGFIFYYGMMLIRTRVLWWPIHPIGFALSSTWCMHHMWFPFLIAA
                     TIKWLLARYAGHTSFRGLAAFALGLILGDIGFGCLWQIYGIIQGVSVWAFWP"
     gene            4451..5410
                     /locus_tag="PLQ54_01750"
     CDS             4451..5410
                     /locus_tag="PLQ54_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS92011.1"
                     /translation="MDKVRIAMIGAGGMANAVHYPSLAEMDDVEFAGICDLDPERLAS
                     TADRYGIDARFSDYRTMIEQTAPDAVYAIMPPHHVFDVAAWCLQQKQNLFIEKPPAVN
                     AFQTRSLAAWAEQSGVKTMVAFNRRFIPVLVRAKELVCQRGEIIQCQASFLKNHLGGG
                     PYYGGAIDILSCDAVHAVDALRWMGGEVAALASVVASYHLPFASAFNAVMRFESGATG
                     VLQTNWSVGKRVHTFELHAAGISAFVDGNGDARIYTDNSEEPRVLVNTEVAGSTEFAH
                     YYGYFAENRHFVDCIKSGRHPMPDLADAAKTMELVERIYASVI"
     gene            5463..>6262
                     /locus_tag="PLQ54_01755"
     CDS             5463..>6262
                     /locus_tag="PLQ54_01755"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS92012.1"
                     /translation="MSATIGQPVEYELMVLGAGPGGYSAALEAARLGARVALIERDEP
                     GGTCLHRGCIPAKTMYEASRHCEPFHEATERRYRVIRELHGQLLKLLTHPKITLIRGQ
                     GFIETPSRVRVTTGDDVQIIAAPRLVVATGSVPIMPDGLPAVGRKMDSDGATFLEALP
                     ARAVVLGGGYTGCEYAGILNTYGVPVVIVELGEHLLMTEDADVVAELTANFTARGIDV
                     RTGTSLEEVELQPTDLLLVTVGRVPESSGVGLEQLGVRFGRRNGLVVN"
BASE COUNT         1032 a         2036 c         2084 g         1110 t
ORIGIN      
        1 ggtcgacgtc tcgatcggga cggacgcgcc ccagcgacaa gccttgccgc tgatgcggag
       61 cgtgtgaaag ctgccgtcgt catggatatc gaatgcaata cgcgcctcgg catcggctgg
      121 ccacttcgct gaggccaccg tgcagcgaac cgcgtactcg ccctcacact ccgagggctc
      181 cggcgtgaac gcgaaagcgg gggtcgcttc ctgggcggcc cgaacggcgg cgggtccggc
      241 cagggccagt gcggcgagga tcagaggcgc aagtctcaac gcgcaccacc cgcccagacc
      301 caggactgga ccgggcgcag gaccggttca tcgggcgaat acgcaagaga aaaatcagta
      361 accggatcgc ccgccgcgtc gaggaagcgg acggagagct gccagagatg gtcattgttg
      421 agcaccttcc ccaccagcac attcagaccg cgcctgagcg taacgccacc cctggtgtcg
      481 cggtctggcg ctagccaacc cgactgagtc gtcatcatca gctctccgtt gagccagatg
      541 cgggcgatgt catcggcccc gacacacagg gtcagattgc tcatggtgtc gttgcagaga
      601 acatacgcca ccccatagcc cagagctccg tcgcagggac cgcccaccac gtggacgaga
      661 tcgagcagga aattgacggt ccggtgcggt gcccatccgt gctgctggtc gccgtgtgtg
      721 acgcgatcac ccggcctggg cctcaggttc gcctcgtcgt cgacgatgta ggtgtcgaga
      781 ctgccgttcg aggggaaatg agtgagagtc acccagtcgc ggatgtaccc gtcatcggtc
      841 ggcacccccg tggctccgcc cgcgccggcc ttggcaagag gccatcgggc cggctgaggc
      901 agcagggaca ggaacagatc gggtatcggt gccccggtct cgtcagcgaa acggacggtg
      961 cacgagaagc caccgccccc gaggaccttg aggactagcg ggtttgtgcc cttgcgaagc
     1021 gatacaccta caaccttgtg tccatcgggc cccatggctc cgggcgcatt gaggatctca
     1081 cccccgttga gccagatgcg cacacccgcg tcagccccac agagcagttg caggcccttg
     1141 aactcgacat cgcacacgat gtacgtcacg gcatacccga tgaacgagtc gactccaggg
     1201 ctcaggcgga cggtcgggtc atcggggcgg caggcgtacc gtgtccacgt cagctcctgc
     1261 ccgccgacgt tgaccgtgtc ccctggcgcc gggcggactt gcgcctcgtc gcgcaagcct
     1321 ggcgcggcaa ggccgcccgg tgcgggcagg ggtgacagca ccaaccagtg ccgcagaaag
     1381 ccatcggccc ggagggcgtg aggggcggga gccgggggga tctccggcgg ctgggcacat
     1441 gctatcccac aaacgacggc cacagctacg agcccgggga ggaagggtcg tgatgcggtc
     1501 ggacgcaacg agcttggcct ccggcgtctc tggcagtcgg gccctcaggc ccactcaacc
     1561 ggacaactga cgaacggcga tgttccattg ccgacagttc gctaccccgg accgggcgcc
     1621 ctctcggccc cggagacagg ccggccacgg gcaggtgcca aggagattcg gggcatcggt
     1681 gacaggttcc gggcacttca tgtgagcgca acgcagccgc gtggcataag tcgtaccaag
     1741 tgtcctcgcc cgcacttggt gacggtggag cccacagacg gcccaccgcg agcgcatacg
     1801 gggcggtccg actcagcccc ctgcctcgcg cggagcggag gtgttcctag catgagacga
     1861 cgcaaaggct tcacgttgat tgagatgatg atcgtcattc tcgtcatcgc ggtcctggct
     1921 accctcgtgg gcgtgtcggt ccgcaatgcg cgcgaacgct cccgtcaggc gacactgacg
     1981 gcccaactca acatcatgca caatgcgatc atgtactacc agaatgacat gagctcctgg
     2041 ccaccggact tgagtgcgct gacatcgacc accaacagcg accccaacta ccacggtccg
     2101 tacctggagc gcgtgccacc gcatccctac cacgggacct ggacctacga ctcgtcgacc
     2161 ggcagggtga cgccctagca gggccagcag gcagcgcgaa gggcgcgcgg tcaggcggga
     2221 gataggctcc cttcggagtg cctggggacg gtccgcaagg cggacgtccc caggcgcttt
     2281 cccaggccgc gcaggaaggt ctcgcgcttc cgacgccgca cctacatcgg cgtgagtccc
     2341 gtatgagcga gcccgcactt ccgccagctg gcccgggtca gggagtccac cccggagccg
     2401 ggtctgagac caacgtgggt gccacgttcc gcgtgtgcct cctggcgctc ctgatcgcac
     2461 ccctcaatgc catctgggtc gttcagatgg acggcgtacg gtcgctgggg atgccgaccc
     2521 tggcggcgcc tttcttcaac gtggtgttca tcatcagcgt ggccgtggcc gtgaatggcg
     2581 ccatgcgccg gctgctcccg cggttcgcac tctcccatgc ggagctcgtg gtcctgtaca
     2641 tcctgatgtc catcctcacg tcgggcgtcg gctacgacac cgtgaacttc actttcgcca
     2701 ccgccaccgg tgcctaccgc tacgggacgc ccgagagtgg ctggcgcgag gccttcttcg
     2761 actacctgcc gtcgggtatg ctcgtcgaag acgccgacac attggaccgc ctctgggcgg
     2821 ggggcgccgg cgacccagcg caccggtgga gctggtcgaa cctggcaggc ccctgggctc
     2881 ggcccgagtg ctggcgaggc gtcattgggc ccatggtgcg atggtgggtt ttcctgtccg
     2941 ctctctggct ggcgccgatg gggctggccg tgatcttccg gtaccgatgg atcgaagccg
     3001 agcgcctgac attccccatc acgtacctgc cgctggagat ctcgcgtccg gacgcatgga
     3061 tgctccggca ccggtacatg tgggccggat tcggcctggc ggcgggtatc agcctactga
     3121 acggactcca cgttctgtac cccacggttc cccagattcc catccggctt gagcaggtgc
     3181 ccatcctcaa cctgggcaac cagctcacag accggccatg gaacgccgtg ggctggttct
     3241 ggctctgttt ctacccgttc gtcatcggga tcgggctcct gctgcccctg gagctgcctt
     3301 tgtcgtgcgc tctgttctat gtgttcttca aggcccagct ggtcttcttc gcatggctcg
     3361 gcatgaccca gaagcccgag ttcccttacc agaaggagca gtcgtacggc ggctacgttg
     3421 ccctcctgct tttctctctg gtggccggcc ggcagtacta catccgggtc gccaagcagg
     3481 tgatcgggct cgtccacatg cccaccgatc ggtccgaacc catgtcgtac cgggcggcga
     3541 cggcgctctt cgtggccggg gcggcctatg tggtcctggt gggcgtgcag cagcacatgg
     3601 cctggtgggt ctcgggtctg ttcttcgctc agtacttcct gttgacagcc atcatcgggc
     3661 ggatccgggc agagacgggc atcccctcgc acgagatcga gcgactggga cccaccgtga
     3721 tgctggggaa tatcctcggg gcgcgcatcc tcggtccccg gaacctgacg ggcgccagcg
     3781 tgttcttcgg cttcacccgt gggatgcgta acataccgtt ccctcaccag gccgaggggc
     3841 tcaagttcgc ggagagactg gggatgccca gccggaggct gatggccgtc atgatggccg
     3901 ccgtgccggt ggggctggcc gtcggcatct gggcacacta tgaccaggtg ttccgctatg
     3961 gctatgctgc cacatggcgg cccgttcacg gctgggcctg ccgcgaggcc tgggaccagc
     4021 tggtggcctg gatcaacact ccggccgggt ttcacagcgg gcgagccacg gcgtgtgccc
     4081 tcggcttcat cttctactac ggcatgatgc tcatccgcac tcgagtgctc tggtggccca
     4141 tccacccgat tgggttcgcg ttgtcctcaa cctggtgtat gcaccacatg tggtttccgt
     4201 tcctgatcgc ggcgaccatc aagtggctgc tggcgcggta cgcgggccat acctcgttcc
     4261 gtggcttggc cgccttcgcc ctcgggctga tcctgggcga cattgggttt ggctgcctgt
     4321 ggcagatcta cggcatcatc cagggcgttt ctgtctgggc tttctggccg tagagggcat
     4381 cggtcggccg gggagaacac cggctcacta cgcctcgcag ccgaacgggg gcgatcatcg
     4441 ggagggcctt gtggacaagg tgcgcattgc catgatcggg gccggtggca tggccaatgc
     4501 cgtgcactat ccctcgctgg ccgagatgga tgatgtggag tttgccggca tctgcgatct
     4561 cgaccccgag cgactcgcca gcacggcgga ccggtacggc atcgacgcgc ggttcagcga
     4621 ctaccgcacg atgatcgagc agacggctcc agatgccgtg tatgccatca tgccgcccca
     4681 ccacgtgttt gatgtggcgg cctggtgcct gcagcagaag cagaacctgt tcatcgagaa
     4741 gccgccggcg gtgaacgcct tccaaacacg gagcctcgct gcgtgggcgg aacagagcgg
     4801 cgtcaagacc atggtcgcgt tcaaccgccg cttcatcccc gttctcgtgc gcgccaagga
     4861 gctcgtgtgc cagcgcggcg agatcatcca gtgccaagcc agcttcctga agaaccacct
     4921 ggggggcggc ccctactatg gcggagcgat cgacatcctg agctgtgacg ccgtccatgc
     4981 cgtcgacgcc ctccgttgga tggggggcga agtggccgct ctggccagtg tggtggcctc
     5041 gtaccacttg ccgttcgcga gtgctttcaa tgctgtcatg cggttcgagt ccggggcaac
     5101 gggcgtcctg cagaccaact ggtcggtggg taagcgcgtg cacaccttcg agctgcatgc
     5161 cgcgggcatc agtgcatttg tcgatggcaa cggcgacgcc cgaatctaca cggacaacag
     5221 cgaggagccg cgggtgctgg tgaacaccga agtggccgga agcactgagt tcgcgcacta
     5281 ctacggctac ttcgcggaga accggcattt cgtcgactgc atcaagtcgg gtcggcatcc
     5341 gatgcctgat ctcgccgacg ccgcgaagac gatggaactc gtggagcgca tctacgcatc
     5401 ggtgatctag gcggggctcg cgcggtgagc tcagccactt cgaccccagg gggtgccttt
     5461 gcgtgtcggc aaccatcgga cagccggtgg agtatgagct catggtgttg ggtgcgggcc
     5521 cgggcgggta ctcggctgcc ctcgaggccg caaggcttgg ggcgcgggtg gccctcatcg
     5581 aacgcgacga gcccgggggt acgtgcttgc accggggatg catcccggcc aagaccatgt
     5641 atgaggcctc gcggcactgt gagccgttcc acgaggcaac cgaacgccgc tatcgggtga
     5701 tccgggagct tcatgggcag ctcctgaagt tgctcacaca cccgaagatc accctcatcc
     5761 gcggccaggg cttcatcgag acgccatcgc gagtgcgggt cacgaccggc gacgacgtgc
     5821 agatcattgc ggcgccacgg ctcgtggtgg ccaccgggtc cgtgcccatc atgcccgacg
     5881 ggctgcctgc ggtcgggcgg aagatggata gcgatggcgc gacgttcctg gaggcacttc
     5941 ccgcccgggc ggtggtcctt ggcggcggat acaccgggtg cgagtacgcg ggtatcctca
     6001 acacctatgg cgtgcccgtg gtcatcgtcg agctcggcga gcacctactg atgacggagg
     6061 atgccgatgt ggtggccgag ttgacggcaa acttcaccgc gcgcggcatc gatgtgcgga
     6121 cgggtacgag cctcgaggaa gttgagcttc agccgaccga tctgctcctg gtgaccgtcg
     6181 ggcgggtgcc ggagtcgtca ggggtgggcc tcgagcagct cggggtgcgt ttcggccggc
     6241 ggaacggtct cgtggtcaac ga
//
LOCUS       DAOSPL010000053         6262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_12579_length_6262_cov_3.252618, whole genome shotgun sequence.
ACCESSION   DAOSPL010000053 DAOSPL010000000
VERSION     DAOSPL010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12579_length_6262_cov_3.252618"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="PLQ54_01760"
     CDS             complement(<1..1180)
                     /locus_tag="PLQ54_01760"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOS92013.1"
                     /translation="MATSRVLVVGTTSDYVQHIHAARPGRALFLTDSAHRAAAVEPAP
                     DAASEVLAELCDHRAATAAVHEHLARHDQRLNGIACYDCESLPLAAELAQCFGLRFAS
                     PQAVAACRNKHESKLRWREAGVPCPSARLVTDLEDVTSFRAATGPWTVMKPLTGSGSE
                     LVFLCRSEAECADALHALRVGLASHPNARMYPDCEGGIHARRVFGAEEYIEGPEMSCD
                     FAIRGGQVRIWRVAQKIKARQTAFGTTAGYEVPAPAPHGLAQRLGEAAAALGLDNCVA
                     MADYILCEQRPVLLEMAPRVGGDCLPDVIRASSGLDTLTAALDLAAGEEPHVPHGRRW
                     SRCLGVRFFAPAAGRVRRLDASPIVSSERILKALTINPKDVVLDSAGRPQPRRQPSID
                     L"
     gene            complement(1170..2123)
                     /locus_tag="PLQ54_01765"
     CDS             complement(1170..2123)
                     /locus_tag="PLQ54_01765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014955848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS92014.1"
                     /translation="MRRLRILTDLGQCRDMWERAFPRERVSDLWEARLCFHHRFRRPL
                     RFVLIEDGHRLAGFLPLAWIEESGCYGYFPGETWAGTTWLEQNRILAREAGDLLALLD
                     AVDGPYHLRYLDPIAAPGTLVPAIDEVGYLFYPPEAGFSMEGYWSAFSGKSAKRIRRE
                     LEALSAPGVSYRFDDPGDFDRLVTLNVSRFGDSSYYADERFREGFRDLMNLLLDRGWL
                     RITSVLIGGEVAAVDMGCAYRGHYTLLAGGTHGGFPGVAKLINLHHIEYACQQRFLSV
                     DFLCGDFGWKSMFHLTPRPLYLLSNLAPAHAAQLQAAGHGH"
     gene            complement(2128..2478)
                     /locus_tag="PLQ54_01770"
                     /pseudo
     CDS             complement(2128..2478)
                     /locus_tag="PLQ54_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002733373.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
     gene            complement(2646..3257)
                     /locus_tag="PLQ54_01775"
     CDS             complement(2646..3257)
                     /locus_tag="PLQ54_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOS92015.1"
                     /translation="MKLLNAARTVFAEKGLDLARIDEITERADVGKGTFYYHFSSKED
                     VIGELIAGLLGELVTAIEERCAGIVNLRDLLDTMIGVHIEFFSNRWEDFVLYFQGRAE
                     LTLQDSYEGLETPFLEYLGHIEDLLAPVIGHQLPRPALRRLACAVAGFVSGYYSFAVI
                     SPQDEDIDETFRSLRGAMASSLARFITEATASGALATGEGARV"
     gene            3600..4826
                     /locus_tag="PLQ54_01780"
     CDS             3600..4826
                     /locus_tag="PLQ54_01780"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOS92016.1"
                     /translation="MRDRAALVEKALRKRLDTVLPLAMREAELDMWLILCQEDNHDPV
                     FNTLIPMDTWCPILQILVFYDRGEQGIERINLSMTGTKDLYDRPWSGQRFEEQWPLLR
                     QIIEERNPRRIGINIGSVQWAAGGLTHNLYLQLTGALGPELSGRLVSAEPAATRWLAT
                     LTDVDIEMHEHVVRVAHRLLAECYSDRAITPGATTTDDLEWYYWQRAADLGLEMAFKP
                     FFTLVRSEESCRRFGADDRTIRQGDFVRSDVGIKYMGLNSDHQQWAYVLRPGERQAPE
                     GFRALMAQANRLQDIFMSEFEAGLTGNEILSRILARAHHEGLPKPKVYSHSLGLYLHE
                     PGPLIGLPWEQEWCEGRGDVRLAENMAFTMELGVRGMVPEWPGQEIGMGLEEDVLFSG
                     GQCRPIDGRQTEFYLV"
     gene            4866..>6262
                     /locus_tag="PLQ54_01785"
     CDS             4866..>6262
                     /locus_tag="PLQ54_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92017.1"
                     /translation="MTMMSAATLALLATAGAEPEGFSLHGRFVGIGACLKTLVAPGPV
                     PGTERLIASHIYGGDTLDIVSVDPATGAAQAFASPVPRETGAWAATVGPDGGVYVGTL
                     PTAHIFRLDWAQGKLIDLGRPSATESYIWQLVVGSDAKLYGCTYPSAKLVRFDPATGR
                     GEDLGRMDETEQYARSVAADDAGFVYVGIGTVLRHVVAYEIATGEHRDVLPAECSGPG
                     TATVTRAVDGRVYAFAGDALVRLEGWTATVIPSGEFPGEPPLKLHDGRTLSYNGPTIT
                     IADPATGAEQTWETGYAGKSQDLFRIALGPDGKLYGSTAMPIHFIWADPDSDEWGEIA
                     RAGGGEFYSLTAHGDVLLGAAYCGDAPLMVYRPAEPWVPGTGAADNPWLVHFEGESPA
                     WRPMALVIGRDGKAYIGAVSGYGLLGGPLCAWDPATGQVEQYPHVITDQSVVSLAVLP
                     GGRIDTDCDDPKSSVV"
BASE COUNT         1065 a         2063 c         2134 g         1000 t
ORIGIN      
        1 gcagatcgat ggaaggttgg cggcgcggct ggggacggcc ggcgctgtcg agcaccacgt
       61 ctttgggatt gatagtcagt gctttgagaa tcctttcgga actgacgatc ggcgacgcat
      121 cgagccggcg gacgcgtccg gccgccggcg cgaagaagcg gacgcccaga caacgtgacc
      181 agcggcgccc gtgtggcacg tggggctcct cgcccgcggc gaggtcgaga gccgccgtca
      241 gcgtatccag ccctgagctg gcgcggatca cgtccggcag gcagtcgcca cccacgcgag
      301 gcgccatctc caggaggact ggccgctgct cgcacaggat gtagtcggcc atcgccacgc
      361 agttgtccag gcccagggcc gccgccgcct cgcccaggcg ctgggcgagc ccgtggggcg
      421 cgggtgccgg cacttcatag ccggccgtcg taccgaacgc cgtctggcga gccttgatct
      481 tctgagccac ccgccagatc cgcacctggc caccacggat ggcgaagtca cagctcatct
      541 cggggccctc gatgtactcc tccgccccga agacccgccg cgcatggatc ccgccctcgc
      601 agtccgggta cattcgcgcg ttcggatggg atgccagacc aacgcgcagt gcgtgaaggg
      661 cgtcggcgca ctccgcctca gagcgacaga ggaacacgag ctcgctgccg ctgccggtga
      721 gtggcttcat caccgtccac ggcccagtgg ccgcgcggaa cgacgtgacg tcctccagat
      781 cggtgacaag ccgcgccgaa ggacacggga cgccggcctc gcgccagcgg agtttggact
      841 cgtgcttgtt ccggcatgcc gcgaccgcct ggggcgaggc gaaccgcagc ccgaagcact
      901 gggccagctc cgcggccagc ggaagcgact cgcagtcata gcaggcgatg ccgttcagcc
      961 gctgatcatg ccgggcgagg tgctcatgca ctgccgcagt cgcggcccgg tgatcacaca
     1021 gttcggcgag cacctctgac gctgcgtccg gtgccggctc caccgccgcc gcgcggtgcg
     1081 ccgaatcggt gaggaagagt gcgcggccgg gacgtgccgc gtggatgtgc tgaacgtagt
     1141 cggatgtggt gccaacgacc agcacgcggc tagtggccat ggccggccgc ctgaagctgc
     1201 gccgcatggg ccggcgcgag attggacagc aagtacaggg ggcgcggggt cagatggaac
     1261 atggacttcc atccgaagtc gccgcatagg aagtcgaccg agaggaaccg ctgctggcag
     1321 gcgtactcaa tgtggtgcag attgatcagt ttcgccacac ccgggaagcc gccgtgggtc
     1381 ccaccggcca gcaaggtgta gtgcccccgg taggcacatc ccatgtcgac ggcggcgact
     1441 tcgccaccaa tcaggaccga agttatgcgc agccacccgc ggtcgaggag gaggttcatc
     1501 aggtcgcgaa agccctcgcg gaacctctcg tcggcgtagt aggagctgtc gccgaaccgg
     1561 ctcacgttca gtgtcaccag ccggtcgaag tcgcccgggt catcgaagcg gtaggagacc
     1621 ccgggggcgg acagcgcctc cagctcgcgc cggatgcgct tggccgactt gccggagaaa
     1681 gcggaccagt aaccctccat gctgaagcca gcctcgggcg ggtagaacag gtacccgacc
     1741 tcgtcaatgg cgggcacgag cgtccccggg gcagcgatgg ggtcaaggta ccgcaggtgg
     1801 tacggcccgt cgacggcgtc gagcagggcc aagagatcgc cggcctcgcg ggcaaggatc
     1861 cggttctgtt cgagccacgt ggtgcccgcc caggtttcgc ccgggaagta gccatagcag
     1921 ccgctctctt cgatccaggc cagaggcagg aaccccgcca gccggtgccc gtcctcgatc
     1981 agcacgaacc gcagggggcg ccggaaacgg tggtgaaagc acagccgcgc ctcccacagg
     2041 tcgctgactc gctcccgggg gaaggcgcgc tcccacatgt cccggcactg gccgagatcg
     2101 gtgaggattc gcagtcgccg cacggtccta gatcctcagg ttcacgtggg cgatgcaatg
     2161 ggtgccgccg aagaaccgct gggcgaactc ctcgaccacc gtcgggtcat agggcttgca
     2221 gctgaacacg tccaggtacg ttgtgttggt ctggttggcg aaatgcgcgg aaatgagcga
     2281 ggtttcaatg agttgtgtca tcgagtagcc cgccacgcgc tcgtcctcgc cgaagtggac
     2341 caccaccgta tcgccgtacc gcgtcatctg gatcaggtca cacagctcga cgacgaactg
     2401 gcggatcttg tcggcatcgc ggatggtctc tggattgcag ttgtagatgt cgatactcgt
     2461 cgcgacgccc caaactccga gttgtgcaaa gtgctcgacc atcgcccctt cggtcgccag
     2521 atccttggtc ggagcctggg gcagtctttc ggcaatggct tgggtttcca ccgcgcaatc
     2581 gctcaccaat cttggtaatg tagtcactgg cgacagaccg tatccgcgtc tctccagggc
     2641 gtgcctcata cacgggcacc ctcgccagtg gcgagagcgc cggaagctgt agcctcggtg
     2701 atgaaccggg ccaggctgga tgccatggct ccccgcaggg agcgaaacgt ttcatcgatg
     2761 tcctcgtctt gcgggctgat gaccgcgaag gagtagtacc ccgagacgaa ccccgccacg
     2821 gcacacgcga gccgcctgag cgccggtctc gggagctgat ggccaatcac cggagccagc
     2881 aggtcctcga tatgccccag gtactcgaga aagggcgtct cgagtccctc gtaggaatcc
     2941 tgcagcgtga gctcggcccg gccctggaag tagagcacga agtcctccca ccggttgctg
     3001 aagaactcga tgtggacacc gatcatggtg tccagcaggt cgcgcaagtt cacgatgcct
     3061 gcacagcgtt cttcgatggc cgtgaccaac tcacccaaca gcccggcgat gagctctccg
     3121 atcacatcct ccttactgct gaagtgatag tagaacgtcc ccttgcccac gtcggcccgt
     3181 tcggtgatct cgtcgatgcg ggccaggtcc aggcccttct cggcgaacac cgtacgagcg
     3241 gcgttgagca gcttcaagac ggtcctttgg gtgcggcgct gttgcctcga acgaggacgt
     3301 ttgccgcgca aagcggggga cgccacagct cgactcccga ctcattggtc gttttcgacc
     3361 atatagtcga aaacgactgg cgagtcaagg ggagttgtgg cgctccgcag ccgacgatgg
     3421 cggatctggc cggtccatgg accggctggc caggcggccc ggcaggagcg gcggctggcc
     3481 gcgaagaacc ccagagcaca ccgggccggc cgcccggcca ctccatccga caggggcggt
     3541 attgcgccgt gatgacaccg acacactccc cgcactgcgt cttccccaag gtgttgccca
     3601 tgcgggaccg cgccgcgctc gtcgagaagg cccttcgcaa gcgcctcgac accgtgctcc
     3661 ctctggcgat gcgcgaggcc gaactggata tgtggctcat cctttgccag gaagacaacc
     3721 acgaccccgt cttcaacacc ctgatcccca tggacacctg gtgccccatc ctgcagatcc
     3781 tggtgttcta cgaccgcggc gagcagggca ttgagcgcat caacctgtcg atgaccggga
     3841 ccaaggatct gtatgaccgg ccctggtccg gccagcggtt cgaggagcag tggcccctgc
     3901 tgaggcagat catcgaggag cgcaatccgc ggcggatcgg catcaacatc ggatcggtgc
     3961 agtgggctgc gggcggcctg acgcacaatc tgtacctgca gcttaccggg gccctgggcc
     4021 ccgaactctc gggccgactg gtgtctgccg agcccgccgc cacgcggtgg ctcgccacgc
     4081 tcactgacgt ggacatcgag atgcacgagc atgtggttcg cgtcgctcac cgcctcctgg
     4141 ccgagtgtta ctccgatcgc gccatcacgc cgggcgcgac gacgacggac gacctggagt
     4201 ggtactactg gcagcgggcc gccgacctcg ggctcgagat ggcgttcaag cccttcttca
     4261 cactggtgcg aagtgaggag agctgtcggc gattcggcgc tgacgatcgc acgatccgcc
     4321 agggcgactt cgtccggtcc gatgtgggca tcaagtacat ggggctcaac agcgaccacc
     4381 agcagtgggc ctatgtcctt cgtccggggg agcggcaggc tcccgagggc tttcgagcgc
     4441 tcatggctca agcgaaccgc ctgcaggaca tcttcatgtc ggagttcgag gccggcctga
     4501 cggggaacga gatcctctcg cgcatcctgg cgcgagctca ccacgagggg ctgccgaaac
     4561 cgaaggtcta ctcccattcg ctcgggctgt atctacacga accgggtcct ctcatcggcc
     4621 tgccctggga gcaggagtgg tgcgaggggc gcggcgatgt acgcctcgcg gagaacatgg
     4681 cgttcaccat ggagctgggg gtgcgcggca tggtccccga gtggccgggc caggagatcg
     4741 gcatggggct cgaggaggat gtgctcttct ccggcggcca gtgtcggccc atcgatggca
     4801 gacagacgga gttctacctg gtgtaggcgc ggccgagtgg aaggcgaacc acgggggcga
     4861 tgccggtgac gatgatgtcc gctgccacgc tggcgctctt ggctacggcc ggggccgagc
     4921 cagaggggtt ctccctgcat gggcggttcg tcggcattgg cgcgtgcctc aagacattgg
     4981 tggcgccggg accggtaccg ggcacggagc ggctcatcgc gtcgcatatc tacggcggcg
     5041 ataccctcga tatcgtgtcg gtggaccccg ccaccggggc cgcgcaggcg ttcgccagtc
     5101 ccgtcccacg tgagaccggc gcgtgggcgg caaccgtcgg ccccgacggc ggggtctacg
     5161 tgggcacgct gccgaccgcc cacatctttc gcctcgactg ggcccagggc aagctaatcg
     5221 acctcggccg gcccagtgcg accgagagct acatctggca gctcgtcgtc ggcagtgatg
     5281 ccaagctgta cggctgcacg taccccagcg ccaagcttgt ccggttcgac ccggccacgg
     5341 gacgaggcga ggacctgggc cgtatggatg agacggagca atacgcgcgg tccgtcgctg
     5401 ccgacgacgc cggtttcgtc tatgtcggca tcggtaccgt ccttcggcac gtcgtggcct
     5461 atgagatcgc cacgggcgag caccgggatg tcctccccgc cgagtgctcc gggccgggca
     5521 cggccacggt gacccgggcg gtcgacgggc gggtgtacgc gttcgccggc gacgcgctgg
     5581 tgcggctcga gggctggacg gccacggtga tcccgagcgg cgagttcccg ggcgagccgc
     5641 cgctgaagct ccatgacggc cggacgctga gttacaacgg cccgaccatc accattgccg
     5701 acccggccac gggcgccgaa cagacgtggg agacgggcta cgcggggaag agccaggacc
     5761 tgttccgcat cgcccttggg ccggacggaa agctctacgg cagcacggcc atgcccatcc
     5821 acttcatctg ggccgacccg gatagcgacg agtggggcga gatcgcgagg gccggcggcg
     5881 gcgagttcta ctcactcacg gcccacggcg atgtgctgct cggggccgcc tattgcggcg
     5941 atgccccgct catggtctac cggccggctg agccgtgggt gccgggcacc ggagcggcgg
     6001 acaacccgtg gctggtgcac ttcgagggtg agagtccggc gtggcgcccc atggccctgg
     6061 tgatagggcg cgacggcaag gcctacatcg gtgcggtctc aggctacggg cttctgggcg
     6121 gcccattgtg tgcctgggat ccggccaccg ggcaggtcga gcagtacccg cacgtcatca
     6181 ccgaccagag cgtggtctcg ctggccgtgc tccccggcgg gcggatcgac accgactgcg
     6241 acgatcccaa gagcagcgtg gt
//
LOCUS       DAOSPL010000054         6126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_12948_length_6126_cov_3.789326, whole genome shotgun sequence.
ACCESSION   DAOSPL010000054 DAOSPL010000000
VERSION     DAOSPL010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12948_length_6126_cov_3.789326"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..635)
                     /locus_tag="PLQ54_01790"
     CDS             complement(171..635)
                     /locus_tag="PLQ54_01790"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HOS92018.1"
                     /translation="MKRQITSLVLFLSTLIMAITGVVMFAMPGHGPGSGGSHFLGLDK
                     GQHKELHVVFMIPLVIAALLHAVWNLGPLVSYLKLRSSTRKHQALGMAVAIAIVLLFA
                     GGTLARMEPFTRFVQLGHRLSHGDDGPGRFPGAPPAANGPTADAMASGVGAD"
     gene            complement(722..3466)
                     /locus_tag="PLQ54_01795"
     CDS             complement(722..3466)
                     /locus_tag="PLQ54_01795"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS92019.1"
                     /translation="MLAMIAMVVGCIGDPGLCQQPAGDPGRRGAGLEAAGLQALTKGD
                     HTRAAARMAQAVEAFRRAGDAHGQGRCLYWLATAEHGGGDLAAASGHLREALELLTEP
                     GQRWLRAYAANRLLAIVSAGSPTDAEAAYAVARQAEAVLDASNPVEALLDRFASTHSS
                     RSPDDAWLFFSTALAAESAVGHDAARLARLEMACAQIARDRKDTGAVARHCENALALR
                     GSVPPSERVKWLLFAADQSTEEPGPVPTRLREALDEARSAGDTALLAQVHLRIAPALR
                     KAGHMDEAVAEFETAATELRDAPWGTPHREDLLGQALIELGEACEAASAGSAAVAAYE
                     EAAAVYEAAGQPREAAGAHHRLGRALAAAGDLAGAECHIEAHLDYYRSLGDPAALLTP
                     LREAGAAYLRAAKADPSAAGARAESCFAEFLRTQARVDGSALARQSLVVATLYANAKL
                     PFQALGYCERARAEGRARGDQLVQAEALATAASAHAARGAYADALACADEAIALVRAA
                     GQEARAAPWLLQSATWARQATGDASQSLDRYERALALYEELGRTAQEAAVCQTLAGLY
                     DSALDDPGIAAQYYRRAAELLTQGGDQGAQRRARQLLPRLAGVLRRSGDDAEAARVLE
                     RCARDQESAGDSPEERARTQEALADILLAGSDFDEARSCLRRALDLYREVSPPSSIAV
                     ATVLLKLAESYHLSGDLASAVEQYEEALASLVGVEAVSGLRAYAYGGLSHALVLLGRH
                     DAAVRARSEAVANERSLAHRLETVLSVVFRPRTASDRPRALAFFERVLEVEDAIGDQG
                     GLRRASACRNIASLYQADGRVAEAEPLLARAAELFATHGWPQEEVVILESLGLIRERL
                     GDWLGATEAYRRAIELYGEMGDPRGRRSELAFVVARILDDHGRPDDAAPYRALAGQDS
                     "
     gene            3688..5964
                     /locus_tag="PLQ54_01800"
     CDS             3688..5964
                     /locus_tag="PLQ54_01800"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOS92020.1"
                     /translation="MALLVLVLASGAHAVPVREVSILGLQGVSEQTVRAVISMREGTQ
                     YDPQLMQRDLRSILDLGLFDERSSKVEPQAQPDGVRVRYLLVENPKIMKIQVDGATAV
                     APEAIIAAVTELVPEGSVLNKHAPALAVQRIQDLYAEQGYHMNMRPASFDRDGTLTLH
                     IIEKSLAKVSVRVMGPSYVDPEVVARWLGPAPGSLLHIPSIQAAADRARSCGLFAGIR
                     PEVELGEPFAPAELTYVVQMLPRPYPTPEVAPMVNAQAVIAGLEYYRDDYVDSHYDPY
                     PPPLPSWITQTTMILDQGGVVDGLGLVMALLRHSGLASARACAARAAAPLRAQVDAGD
                     TNPQLLINLARLELVQNHTDSALALASRAFESGAFPPETYATLLQAAVMELARGAATQ
                     KGAAVPDDPDLAWAVWWLSGYVDSDPILTQGPAAWIRPAVSRGLDHFDSLNEDGLLQE
                     LPAYLRFLHLCDFLKIVPAPIRPADVDPRLADRIGLPLSPTHSDRRVLPLLTARAALE
                     PPDWRIRYAYANCTVHREIGLAFLGADSATKLGFPPAERAQRLATAEQYLQAMVQSAD
                     AEYPWARALLAISRALQGDVATARALLLPALDGPVDIGASHLYANVITLDQLANARDE
                     AYVKEQLTTALAEIQTRLATASGPMPGVRASQTRIESMLGLFDQALATAKRLAEALPE
                     AEPLAMQGFLELKMAKVPEAVRTAERAVELDPDDPFIQYTLGLALLAADQPEKAAPHL
                     RAIERYTLDHRLAGLLGA"
BASE COUNT          979 a         2200 c         2014 g          933 t
ORIGIN      
        1 cacgggttcg gagcagcggc gatggcacac ggacaccggg gagttctgcc agcgccgccc
       61 ccccaactgc atccatcggt attcgctcga aggggccagc ggtcctcgcc tggccccacc
      121 aggtgcgcgg gttacccgct ggccgtggtg tcccgtcgtg cagtgtcggc tcagtcagca
      181 cccacacccg atgccatcgc atccgcagta ggcccattgg ccgcgggcgg tgctccggga
      241 aaccggccgg gcccgtcgtc gccgtgcgac agccggtggc caagctgcac gaaacgagtg
      301 aagggctcca tacgcgccag agtgccgccg gcgaacagca gcacgatggc gatcgcgacc
      361 gccatgccga gggcctggtg cttgcgggtc gacgaccgca gcttgaggta gctcaccaac
      421 ggcccgagat tccagacggc atggagcagt gcagcgatca ccagcgggat catgaacacg
      481 acgtggagtt ccttgtgctg ccccttgtcg agcccaagaa agtgcgaacc gccagatcct
      541 ggcccatggc caggcatcgc gaacatgacc acaccagtga tggccatgat gagcgttgac
      601 aggaagagta cgagcgatgt gatttgccgc ttcacagagt atactccttc tctcacccgc
      661 ctctgcgggc ctgacggtac tgcttctgac ggagccgccg aggtccggtt cggttgtgca
      721 ttcaactatc ttgcccagcg agggctcgat acggggccgc gtcatccggc cttccgtgat
      781 cgtcgagtat tcgagcaaca acaaacgcaa gctccgagcg acgtcctctc ggatcgccca
      841 tctccccgta gagctcgatg gcgcgccggt aggcctccgt agccccaagc cagtcaccga
      901 ggcgttctcg gatgagtccg agtgactcga gaatgacgac ctcctcctgg ggccagccgt
      961 gcgtagcgaa gagctcggcg gcccgggcga gtaacggctc ggcctccgcc acacggccat
     1021 cggcctggta cagactggcg atgtttctgc aggctgatgc ccgcctcaag ccgccttggt
     1081 caccgatagc atcctcgacc tccagaaccc gctcgaaaaa ggccagcgcg cgcggacgat
     1141 cgctcgcggt gcgcggacgg aacacgaccg atagcacggt ctcgaggcgg tgagcgagac
     1201 tccgctcgtt ggccaccgcc tcgcttcgcg cacgcaccgc cgcgtcgtgc cggcccagga
     1261 gaaccagagc gtggctgagt cctccatacg cgtaggccct caggccgctc accgcctcga
     1321 cacccaccag gctcgccagc gcctcctcat actgctccac ggcggacgcc aagtcaccgc
     1381 tcaggtggta cgactcagcc agcttgagca gcaccgtcgc gacggcgatg ctcgatgggg
     1441 ggctcacttc gcggtagaga tccagcgccc gccgcaggca cgagcgggct tcatcaaagt
     1501 ccgagcccgc cagcagaatg tcggccagag cctcctgggt ccgcgctcgc tcctcggggg
     1561 agtcacccgc agactcctgg tcgcgggcgc accgctcgag gacgcgagcc gcttcggcgt
     1621 cgtcacccga gcggcgcagc acaccggcta gtcgggggag cagctgccgg gcgcgccgct
     1681 gcgccccctg gtctcccccc tgggtcagca gctcggccgc gcgccggtag tactgggcag
     1741 cgatacccgg gtcgtcgagg gccgaatcat agagcccggc cagcgtctgg cagaccgcag
     1801 cctcctgggc ggtacgtccg agttcctcat agagcgccag ggcgcgctca tagcggtcga
     1861 ggctctgaga cgcatcgccc gtggcctggc gagcccaggt tgccgactga agcagccacg
     1921 gggccgcacg ggcctcctgc ccggccgcgc gcaccagcgc gatggcctcg tcagcacaag
     1981 ccagcgcatc ggcataggcg ccccgggcag catgggctga cgccgccgta gccaacgcct
     2041 cagcctgaac cagctggtcg ccccgagcgc gcccctcggc ccgagcacgc tcgcaatacc
     2101 ccagcgcctg gaacggcaac ttcgcattgg catagagcgt ggccacgacc agcgactggc
     2161 gcgccagggc ggacccgtcg actctcgcct gtgttcgcag aaactcggcg aaacagctct
     2221 cagcgcgggc gccggccgct gacgggtcgg ccttggcggc acggagataa gccgcgccgg
     2281 cctccctgag cggtgtgagg agggccgccg gatcgcccag cgagcggtag tagtccagat
     2341 gggcttcgat gtggcactcc gcaccggcca ggtcgcctgc cgccgccagg gctcgcccga
     2401 gccgatgatg ggcacccgcc gcctcgcgcg gttgcccggc agcctcatag acggcagcgg
     2461 cttcctcata ggcggcaacg gccgcagatc cggcactcgc tgcttcgcag gcctccccca
     2521 gctcgatcag tgcctggccc agaagatcct cacggtgtgg tgtgccccat ggcgcgtccc
     2581 gcagctcggt ggctgcggtc tcgaactcgg cgacggcctc gtccatgtgg ccggccttcc
     2641 ggagcgctgg cgcgatccgc aggtggacct gggccagcaa cgccgtgtca ccggccgacc
     2701 gggcctcgtc caaggcctcg cgtagccgcg ttggcaccgg gccgggctct tctgtcgact
     2761 gatcggccgc gaagagcagc cacttcactc gctcggaggg cggaacgctg ccgcgcaggg
     2821 ccagcgcgtt ctcgcagtgc cgtgccacgg ccccggtgtc cttccggtcg cgcgcgatct
     2881 gggcgcaggc catctcgagc cgggccaggc gagccgcatc gtggccgacg gcggactcgg
     2941 cagccaaggc agtcgagaag aagagccagg catcgtccgg gctcctcgag gaatgggtgc
     3001 tcgcgaaccg gtcaaggagc gcttccaccg ggttcgaggc atcgaggacc gcttccgcct
     3061 ggcgcgctac ggcgtaggcg gcctcagcat cggtcgggga cccagcgctg acgatggcca
     3121 agaggcgatt tgccgcgtac gcgcgcagcc agcgctgccc cggctcggtg agtagctcga
     3181 gcgcttcccg caggtggccg gaggctgctg ccaggtcgcc gcctccgtgc tcggccgtgg
     3241 ccaaccagta gaggcaccgc ccttgcccat gggcgtcgcc cgcgcgccgg aacgcctcga
     3301 cagcctgcgc catccgcgcg gcggcacggg tgtgatcgcc cttggtgagg gcctgcagcc
     3361 ccgcggcctc gagccccgct ccgcggcggc ccgggtctcc ggcgggttgc tggcacaagc
     3421 cgggatcacc gatgcaccca acgaccatgg cgatcatcgc cagcatgcgg aaacgccggc
     3481 cgggcccgcg aaaccaaccc ctcgcggcgt ctggtggccg atgggacgac ggcggtcggg
     3541 gtagcacgtc tgcactgcct ccttgactcc ggcctactcc tcaccggaca gtataggggc
     3601 tccggcagct cgctggggtg acgcgagggc atgggaggct tccacccgat gagtgtgcgg
     3661 cgctctaggc gcctgcgagt cccatgcatg gcgctgctgg tactcgtgct ggcttcgggc
     3721 gcacatgccg tccccgtgcg cgaagtgtcg atccttggcc tccagggcgt gtccgagcaa
     3781 accgtccgcg cggtcatctc gatgcgcgag ggcacccaat acgatcccca gctcatgcag
     3841 cgcgacctgc gcagcattct ggacctcggg ctgttcgacg agcggagctc caaagtggaa
     3901 ccccaagcac agcccgatgg cgttcgggtc cggtatctcc tggtcgagaa cccgaagatc
     3961 atgaagatcc aggtggacgg cgccacggcc gtggcgcccg aagcgatcat cgccgccgtg
     4021 accgagctcg ttcccgaggg ttccgttctc aacaaacatg ccccggcact ggccgtgcaa
     4081 cggattcagg acctctatgc cgagcaggga taccacatga acatgcgccc ggcgagcttc
     4141 gaccgggacg gcacgctcac gctgcacatc atcgaaaagt cgctggcgaa ggtctccgtg
     4201 agggtaatgg ggccgagcta cgtggacccg gaggtggtcg cgaggtggct gggccccgcg
     4261 ccggggagtc tcctgcatat tccctcgatc caggccgcgg cggatcgggc acggtcgtgt
     4321 gggctgttcg cgggcatacg gcccgaagtg gagctgggtg agccgttcgc gccggccgag
     4381 ctcacctacg ttgtccagat gctgccgcgc ccctatccca cgcccgaagt ggcgcccatg
     4441 gtcaatgctc aggcggtcat cgccggcctc gagtactatc gcgatgacta tgtcgatagc
     4501 cactatgacc cgtacccgcc tccactcccc agctggatca cgcagacaac gatgatcctg
     4561 gaccagggcg gcgtcgtcga cggcctggga ctcgtgatgg cgctgctgcg ccactcgggg
     4621 ttggccagcg ctcgggcgtg cgccgcccgg gccgccgccc cgctgcgtgc ccaggtcgat
     4681 gcgggcgata cgaacccgca gctcctcatt aacctggcgc gtctcgaact ggtgcagaac
     4741 cacaccgact ccgccctggc actggcctcg cgggctttcg agtcgggcgc cttcccaccg
     4801 gagacgtacg ccacactcct ccaggccgcc gtgatggagt tggcacgcgg tgcggccacc
     4861 cagaagggcg ccgcagtgcc ggatgacccc gatctggcat gggccgtgtg gtggctgtcg
     4921 ggctatgtgg actcggaccc gatcctgacg cagggcccag cggcctggat ccggccggcc
     4981 gtgtcgcgcg gtctcgacca cttcgactct ctcaacgagg acgggctgct ccaggaactc
     5041 cccgcgtacc tccgcttcct gcacctgtgc gacttcctca agatcgtgcc tgcgcccatc
     5101 cgcccggcag acgtcgaccc aaggctggcg gaccggatcg gactcccgct ctcaccgact
     5161 cactcggacc ggcgcgtgct tccgcttctg accgcccggg ccgccctgga gccgcccgac
     5221 tggcgaatcc gctatgccta cgccaactgc acggtgcatc gcgagatcgg cctggcgttc
     5281 ctgggcgcag atagcgccac caagctgggg tttcccccgg ccgagcgggc ccagcggctg
     5341 gccaccgcgg agcagtatct gcaggcgatg gtccagagtg ccgacgccga gtacccgtgg
     5401 gctcgggctc tgctggcgat ctcccgggcg ctgcagggcg acgtcgccac tgcccgggcg
     5461 ctgctcctgc cagccctgga tggacccgtg gatatcggcg ccagccacct gtacgccaat
     5521 gtcatcactc tcgatcagct cgctaacgcc cgagacgagg cctatgtcaa ggaacagctc
     5581 acgacggcct tggccgagat tcagactcgt ctggccacgg cgagcggccc catgcccggc
     5641 gtccgggcct cccagacgcg catcgagtcc atgctcggtc tgttcgatca ggctctggcc
     5701 acggccaagc ggctcgccga ggccttgccg gaggcggagc cgctggcgat gcagggcttt
     5761 ctggagctga agatggccaa ggttcctgaa gccgtacgaa ctgccgaacg cgccgtcgag
     5821 ctggatcctg acgacccgtt catccagtac acgctgggcc ttgcgctcct ggccgccgat
     5881 cagccggaga aggcggcgcc ccatcttcgc gccatcgagc ggtacaccct ggatcaccgc
     5941 ctggccggcc tgctgggcgc ctgaacgcca aggacccgac tgcgccgccc ccgaagacac
     6001 ccgcacagca ctccaccaaa tactcccgcc gcgcgaggcg aatgtgatgg gtccaaagct
     6061 ccaactcggg ccgctcgatt ccgtcatcgt caatgacagc gcaggctact tctttccagg
     6121 aacaac
//
LOCUS       DAOSPL010000055         6119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_12972_length_6119_cov_3.175956, whole genome shotgun sequence.
ACCESSION   DAOSPL010000055 DAOSPL010000000
VERSION     DAOSPL010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12972_length_6119_cov_3.175956"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="PLQ54_01805"
     CDS             <1..1209
                     /locus_tag="PLQ54_01805"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HOS92021.1"
                     /translation="AYGQRIRPGERLDFPGLGMVYFRLAWGYLEPEEGHFDWSWIDPW
                     YQQFVNQGLKVGFRITCFESGGIPYATPKWVEDAGARFFPFADPNSPQRQPGWDDPVF
                     LSKLDAFLAAFAARYDADPNVAWIDIGSLGVWGEGHAGWSSTGPIPLEVYQKHIDLHY
                     KHFRNALLVVNDDFGHEAVRYACGLGAGLRDDSICVNAEHPYFHQDMADWFWRSRPVI
                     VETEHYGPSLERGAWDSGTVERSVEDYHASWVSIHHWPDEFLAKERPLVERLANRMGY
                     WLFVPEARMPAVLSPGVPFTVSVRWENRGVAPLYTNYAVALGLKDAAGRVVWSGADER
                     WDLRFIGPGESLETVHSHPFPEGLEAGTYTLCLGLSPHDEPNTPAVALAMAGRDADRW
                     HPLGPIELLP"
     gene            1366..1557
                     /locus_tag="PLQ54_01810"
     CDS             1366..1557
                     /locus_tag="PLQ54_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOS92022.1"
                     /translation="MARVPTVNQDECTSCRLCTEIAPNTFDVDGNDLAYVKNPTGDSE
                     SEIQEAIDSCPVSCISWEG"
     gene            2102..5779
                     /locus_tag="PLQ54_01815"
     CDS             2102..5779
                     /locus_tag="PLQ54_01815"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS92023.1"
                     /translation="MREAERPAVDEDASDQAAVLQSRVSSQRLARARAESLAQSPGER
                     RTATVLMLDVSGYTAMSETLAPEDIQYIINAWHDVLVRIITDEYAGWINKTMGDGVMA
                     TFGAPIAVDNAPERAVRAALRIQEAIEDFAPEGIDMSGIHLRVRIGIHSGEVVAGGVM
                     ADGVRKFDVLGSTVNIASRLESSAPVGGVLVSDAVYRRVSDLFEFEPMGPLKLKNVSE
                     PVLAHLVTGVRKRTTRIDAAQRLGLSPLVGRDRELRQLISAAERSRAGEGQLVSIIGE
                     AGVGKSRLVFELKRSLGGPDEVLWLQGQFLSFGSNVAYLPFQDALRELAGIEPRDTQD
                     VQRSKLDAALGRLACEAAVELAPALGAILSIDYPGSWFDRLPDDQRGEEIRRAVTELI
                     FGLAADRFVVLHLDDVHWADNSSDLLIQRIIQELNEHAVLLIECHRPQHETAWDGVDR
                     RVIQLERLSEGDSSKLLASLVGSQRLGPTALAKVLKNADGNPFYLEEFVRAIELSQPD
                     ESGEIVLDRIPDTVQEIIDTRLDRLAVESPAAWSFLKTAAVVGSRFSAELVERSRGMD
                     SAERVECMNLLIERRFLRMTESAGASERAPAGEFAFEHNLTHEQVLARTVTRERQGLH
                     RDVAEAMSQLYAAQLDRYAGQLARHYRDGGLPAKALPHYHTAVGLQLRSGANAEVIEN
                     ATVALELLEQVGDIDRSQLYWSEFLLWRAHAQVVLGNAHQAIADARRGLEAARKPRQP
                     DFEARAYERIAFAFLRLGEFERAKQYYRLAERMFVVAGQPERAGVCRQGVISILHDTG
                     DYEAARRAYEEELQLRRSREKSADDWRDELSLLVNLGSTLEKLGEYQKALEYTMEADR
                     VCADINDSLGRAFMTANIAELQRHMGRYDEARKAVRDALAMFGEMGDRSMEGFLHKDL
                     AQIEIELGEYQPALGHARDSLNIGLEVEAGALQADAMVAQAAASMFMGLLDSSWDIAL
                     GALDLARSVGSVQAEADCALVFGALATERGQLDDAGEAYDRALQRAREMKNRRAEAEA
                     LAGHGRVYLAMGDLDAARAAFDQSRQIAEPMGARRVLADALAGTARALELQDASDQAR
                     EAFQRALTLAEDMGQARLIAEAAAGLGRCRRRAGDRQGALDLFVKAIQVAKRSAATCG
                     PFAARSLPARMLCEVQTQTISLLWEMGAVAKPMADIDSGLLRLIERPADDPEELRNRY
                     PELDSLATDLLRQCGVDVSWN"
BASE COUNT          955 a         1968 c         2121 g         1075 t
ORIGIN      
        1 gcctatggcc agcgcatacg gccgggcgaa cgcctcgatt tcccgggcct ggggatggtc
       61 tacttccgtc tggcgtgggg ctacctcgaa cccgaggagg ggcacttcga ctggagttgg
      121 atcgacccgt ggtaccagca gttcgtcaac caggggctga aggtcggatt tcgcatcaca
      181 tgcttcgagt cgggcggcat cccgtacgcg acccccaagt gggtcgagga cgccggggcg
      241 cgtttcttcc cgttcgcgga tccgaactcg cctcagcgac agccaggctg ggatgatccc
      301 gtgttcctga gcaagctgga tgcgttcctg gctgctttcg ccgctcggta cgatgccgac
      361 ccgaatgtgg cctggatcga tatcggctct ctgggcgtct ggggtgaggg ccatgcgggc
      421 tggagctcga ctggccccat ccccctggag gtctatcaga agcacatcga cctccactac
      481 aagcatttcc ggaacgccct tctggttgtg aacgacgact tcggccacga agcggttcgc
      541 tacgcttgcg ggctgggggc cggcctgcgg gatgactcga tctgcgttaa tgccgagcat
      601 ccctacttcc accaggacat ggcggactgg ttctggcgaa gccggcccgt gatcgtcgag
      661 acggagcact acgggccttc gctggagagg ggagcctggg actctgggac ggtcgagcga
      721 tccgtcgagg actaccacgc gtcgtgggtc tcgatccacc actggccgga tgagttcctg
      781 gcgaaggagc gcccactggt cgaacgtctg gccaatcgca tgggctactg gttgttcgtg
      841 ccggaagctc ggatgcccgc cgtgctgagc ccgggcgtgc ccttcacggt gtcggttcgg
      901 tgggagaacc ggggcgtggc gccgctgtac accaactacg cggtggcact cgggctgaag
      961 gacgccgccg gtcgggtcgt ctggtccggt gccgatgagc ggtgggatct gcgcttcatt
     1021 gggcccggcg agagccttga gaccgtacat agccacccgt ttccggaggg tctcgaggcg
     1081 ggcacgtaca cgctctgcct cgggctgtcg ccgcatgatg agcccaacac tcctgccgtg
     1141 gcattggcga tggccgggcg cgacgctgac cgttggcatc cgctgggtcc catcgagctc
     1201 ctgccctgag tctgggctca caggtatgcc ggcatcggcg gggaacttcg ggctataatc
     1261 gggcacaatg cttggcgtat ggagatgcgc tgggcctgtg gcatcagtcg ttccgcaacc
     1321 tgtagcactg ctattcggtg cagctaaagg agaggtcgtt ccccaatggc acgcgttccc
     1381 acggtgaatc aggacgaatg cacgagctgc cggctatgca cggagatcgc gccgaacacc
     1441 ttcgatgtgg atggcaacga tctggcgtac gtgaagaacc ccaccggcga ctcggagtcc
     1501 gagatccagg aggcaatcga tagctgcccc gtctcctgca tctcctggga aggctagcgg
     1561 ctgtgtcgac ctcgcttggg gccgggatgc ttcccggccc caaatcgagg tgcatcttga
     1621 cattcatgca gtgctaggct aacctgctcg aacgcggccc ggcgccaggg gaaccccccg
     1681 tcatccaagg ggtcttggta tgcagtgccc caggtgcaat gccgctattc ggggcgagca
     1741 ccgcttctgc acgcaatgcg ggcagtgtgt ggtgtcgccg tgtgggcgtt gcggcgccga
     1801 ggtccggatc ggcgaccgct tttgtggcat gtgtggagtc gctcaggtcg cggtcccgac
     1861 tgacgtgcca ctgcccgccc ctcccgttcc gccgacggcc tcgtatgtgc cctgggccgt
     1921 agccccgggg cctccctcgt ttgtctatgc cgtgccccct caggcctggg ttcagggcca
     1981 gccacttccg atggtgccgc cggtgcccgc gccccccgtt catggggggc cttcgccggc
     2041 cgaggcggta ccggctccgg agcctgagtc cgtgccttct gtcccgccca ctgtggagcc
     2101 gatgcgggaa gccgagaggc ccgcggtgga cgaggacgcc tccgatcagg cggcggtgct
     2161 gcagtcgcga gtgtcgtcgc agcgcctcgc ccgggctcgg gctgagtctc tggcgcagtc
     2221 gccgggggaa cgccgcacgg ccaccgtgct catgctggac gtcagcggct acacggcgat
     2281 gtctgagacg ctggctcccg aggatatcca gtacatcatc aatgcatggc atgacgtctt
     2341 ggtacgcatc atcacggacg agtatgccgg ttggatcaac aagaccatgg gggacggcgt
     2401 catggcgacc tttggtgcgc cgatcgccgt cgacaatgcg ccggagcgtg cggttcgcgc
     2461 ggccctgcgg attcaggagg ccattgagga ctttgcccct gagggcattg acatgtccgg
     2521 catccatctc cgggtccgca tcggcatcca ctcaggcgaa gtggtcgcgg gcggtgtgat
     2581 ggccgacgga gtacggaagt tcgacgtgct cgggtcgacg gtgaacatcg cttcgcggct
     2641 cgagtccagc gcgccggttg ggggcgtcct ggtcagcgac gccgtgtacc ggcgcgtgtc
     2701 ggacctcttc gagttcgagc ccatgggccc gctgaagctc aagaacgtga gcgagccggt
     2761 gctcgcccac ctcgtcaccg gagtccggaa gcgcacgacg cgcattgatg cggcccagcg
     2821 gctgggtctt tcgcctctcg tcggacgcga ccgggagctt cgccagctca tctcggccgc
     2881 cgagcgcagc cgggccggcg aggggcagct ggtgtcgatc atcggcgagg cgggtgtggg
     2941 caagtcgcgt ctcgtcttcg agctcaagcg ttcactgggc gggccggatg aggttctctg
     3001 gttgcagggg cagtttctct cctttggctc gaacgtggcg taccttccct ttcaggatgc
     3061 cttgcgcgaa ctggcgggaa ttgagccgcg cgacacgcag gatgtccagc ggtccaagct
     3121 cgatgcggca cttgggcgtc tggcttgcga ggccgcggtc gaactggcac cggctctggg
     3181 cgccatcctg tccatcgact acccgggctc gtggtttgac cggctgccgg atgatcaacg
     3241 tggcgaagag atccgccgag cggtgaccga gctgatcttc ggtctggcgg ccgaccggtt
     3301 cgtcgttctc catctcgatg acgtccactg ggccgacaac agctcggact tgctcatcca
     3361 acgtatcatc caggagttga acgagcatgc ggtgctgctg atcgagtgcc accgaccgca
     3421 gcacgagacc gcctgggacg gcgtcgaccg gcgggtcatc cagctggagc gcttgtcgga
     3481 gggggacagc agcaagctgt tggcgtcgct ggtggggtcc cagcggctcg ggcccacggc
     3541 cctggccaag gtcctgaaga acgcggacgg caaccccttc tatctcgagg agttcgttcg
     3601 agccatcgag ctcagccagc ccgatgagag tggcgagatc gtcctcgacc ggatccccga
     3661 tacggttcag gagatcatcg acacccgact tgaccgtctg gcggtcgagt ccccggctgc
     3721 gtggtcgttc ctcaagacgg cggccgtggt cgggtcgcgt ttctcggccg aactggtaga
     3781 acgctcgcgg ggaatggact cggccgagcg agttgagtgc atgaacctgc tcatcgagcg
     3841 gcgcttcctg cgcatgactg agagcgcggg ggcctcggag cgggctccgg ccggcgagtt
     3901 cgcgttcgag cacaatctca ctcatgagca agtgctggcg cgaacggtga cgcgcgagcg
     3961 gcaggggctg caccgggacg tggccgaggc catgagccag ctctacgccg ctcagctcga
     4021 ccgctacgcc ggtcagttgg cccggcacta ccgcgatggg ggactcccag ccaaggcgct
     4081 gccccattac cacacggcgg tgggtctcca actccggagt ggcgcgaatg cggaggtcat
     4141 cgagaacgcc acggtcgccc tggaactact ggaacaagtg ggggacatcg accgctccca
     4201 gctgtattgg tcggagttcc tcctctggcg cgcccacgcc caggtggttc tgggcaacgc
     4261 gcaccaggcc atcgcggacg cccggcgagg gctcgaagcg gctcgcaaac cgcgccaacc
     4321 ggactttgag gcgcgcgcct atgagcgcat cgcgttcgcc ttcttgcgtc tgggcgagtt
     4381 cgagcgcgcc aagcagtact accgtctggc cgagcgcatg ttcgtggtgg ccgggcagcc
     4441 cgagcgggcg ggagtgtgcc gacagggcgt catctcaatc ctacacgaca cgggcgacta
     4501 tgaggccgct cgccgggcct atgaggagga gttgcagctc cggcggagcc gtgagaagag
     4561 cgcggacgac tggcgcgatg aactcagtct gcttgtcaac ctgggaagca ctctggagaa
     4621 gctcggtgag tatcagaagg ccctcgagta cacgatggag gcggatcggg tctgcgcgga
     4681 catcaacgac agcctgggtc gcgccttcat gacggccaac atcgccgagc tgcagcgcca
     4741 catgggtcgt tacgacgagg cccggaaggc ggtgcgggac gcgctcgcca tgttcggaga
     4801 gatgggcgac cgctcgatgg aaggtttcct ccacaaggat ctggctcaga tcgagatcga
     4861 gctgggtgag taccagccgg ctctcgggca cgcgcgcgac tcactcaaca ttggtctcga
     4921 ggtcgaggcc ggcgcgctgc aggccgacgc catggtcgcc caggcggccg cgagcatgtt
     4981 catgggcttg ctggacagca gttgggacat cgctctgggc gctctggacc tggcccggtc
     5041 ggtggggtcc gtgcaggccg aggccgattg tgcgctggtc ttcggtgccc tggccacgga
     5101 gcgaggtcag cttgatgatg ccggcgaggc ctatgatcgc gcactccagc gcgcccggga
     5161 gatgaagaac cgccgcgcgg aagccgaggc cctggccggc catggccggg tctacctggc
     5221 catgggcgat ctcgatgccg cccgggcggc tttcgatcag tcgcgccaga tcgccgagcc
     5281 catgggcgct cgtcgagtcc tggctgacgc gctggccggc accgcacggg cgctggagtt
     5341 gcaggatgct tcggaccagg cacgcgaagc gtttcagcgc gccctgacgc tggcggaaga
     5401 catggggcag gctcggctga tcgcggaagc cgccgccggt ctgggccgct gcagacgccg
     5461 ggcgggcgat cgccagggcg cgctggatct gttcgtcaaa gcgatccagg tcgccaagcg
     5521 gtcggcggcg acctgcggcc cgttcgcggc tcggtcgctt ccggcccgca tgctgtgcga
     5581 ggtgcagacc cagaccattt cattgctgtg ggagatgggc gccgttgcca aacccatggc
     5641 tgacatcgat agcgggctcc tgaggctcat cgagcggccc gctgacgatc ccgaggagct
     5701 gcgcaaccgc tatcccgagc tggactcgct cgccaccgat ctcctccgcc agtgcggcgt
     5761 cgacgtgagt tggaactgaa taacgccagg gaggctcggc gcatcgcgcc tccgcccagg
     5821 cccctcggcc tccgtccaca cgccccgcgg caccgtgggg cacgattggt cgtacaatgg
     5881 ccgtagcaag gcccgagcca gcgcacacgc gggacccaac cgccgggtgg gagggagcgg
     5941 ctccatgcag tgtccacggt gcggtttcac actctacgaa caccactcgg catgcccatg
     6001 gtgtgggcac ccgatcacgc ttccgcccga cacagagccc gaggctggcc gtggtcacga
     6061 cctgttgggc ccgagcccgc tgccgccccc ggagcccgct cccgcgcctc ccccccccc
//
LOCUS       DAOSPL010000056         6105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_13016_length_6105_cov_2.720826, whole genome shotgun sequence.
ACCESSION   DAOSPL010000056 DAOSPL010000000
VERSION     DAOSPL010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13016_length_6105_cov_2.720826"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="PLQ54_01820"
     CDS             <1..1291
                     /locus_tag="PLQ54_01820"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92024.1"
                     /translation="GAYHVLRSQLPALPVPAGSRRAVCTAVDGTERSGRSDTTVLADA
                     AGPPEGVTHLAADASTPFAVHLTWQPGAAGQRVRYEVHRGPEGFAPSAQTLLGETALT
                     EFIDTTPPLGQRVAYAVIPVDAAGRMGPVSECVAEAPSPPLLPAVTGVRATSGPGRAV
                     VSFDPLPDRGVVGYRVQRQAGTEWVSAAGATPLRGVSLVVAPLEPGRPERFRVVAVDR
                     AGRDGAPSEPVTVTATAAPIEPVFATSFGGTSAETGQQGQLQGGATVADGCLDTREGG
                     WIAFPNQDTLQLGGPLSFDLWIRPASLEGIPVFLSYGHWEGLGYWLQFIGGKLRYYLP
                     RQQILDAGSIPVGEWSYIAAVFTGSEQILYVNGQEVGRRGVPTLDAQPWPGELRIGQY
                     SDIDAQFQARALIDDVRIYQRPLSAEEVRAAYEQGRR"
     gene            complement(1378..3450)
                     /locus_tag="PLQ54_01825"
     CDS             complement(1378..3450)
                     /locus_tag="PLQ54_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92025.1"
                     /translation="MTFSALCACAVLAGAPPALQWSEALLSRPSAWSDRMNGALVTPY
                     RGALRVEIAPGCAWAIAAIPSACLPPGTERVTASVRSLSEGSRWLMRLFGDVRGTGRP
                     ATSSLAEQCARTGQTILEPDPRLMLLGHGQPVQLQLGIEGKPGDSVVFEGLEFASGAP
                     SPRGATIPGQLPIAAVDLMPSLPQPYRMLDWRAKARAYDRLAFDWSARGQFLPLIWLD
                     ESRINVDAPTFGLPSYVGDPRQGTNGQESITCMGAVLGATVAGIDKREGPHDYVAMCQ
                     AFANRRNGSNLVLNLQDQGTGGSYWYDLFPHIVFYALADRYPDAGLEPVVRDTADRWA
                     RVVDAVDATFEHTGFDFASGKPVDNGKWREPDAAAGIAWMEYAAWTRWRDPAHLRAAQ
                     RCLRFLEERESNPYYECLLPWGVQAAAKMNAELGAEWDVDRLLNWCFDISDCRGGWGA
                     IVQNWGGYDCAGLVGSVDNRGGYAFAMNTFSQAAALTPVARYDPRYARALGKWMLNLA
                     NAARLFYPTELPTSNQDCGWWQGDPEGAIAYEGLRRQRDGRSPFGTGDPIEFRWGPKT
                     NLGLYGSSYVGLLGGIVSTTDVPGILALDCLATDFYRARAYPTHLYYNPHAAARMITV
                     PLAVEPTDLYDAVSHRFLVEGTHGDAKVRIAGDRALVLVSVPSGSHRETVGGRLLCDD
                     VVADYRAR"
     gene            complement(3639..4379)
                     /locus_tag="PLQ54_01830"
     CDS             complement(3639..4379)
                     /locus_tag="PLQ54_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92026.1"
                     /translation="MILTCLVAASAEDPPAAQPAPEQWIAPLPDDVDRAVGEDWVEEW
                     MGKWVVWPSEDEVRARGPLRDRRAEFPAEGADGKPIVRGDPGAVSPDEARADAAAWVR
                     RILKPELVPDDLDDGFVLLQEEDPSRSSVLCRFEIHGTGIQVTQLRWALCVVMRPDPA
                     LIEGLEPRAIGPAAFRAVFTKGDAMAGARFSDVPGLPYFAQELDLSVSSALWWGGFFW
                     YTDGRSTAVYIRRADRGHSPARAGDPWF"
     gene            complement(4584..5369)
                     /locus_tag="PLQ54_01835"
     CDS             complement(4584..5369)
                     /locus_tag="PLQ54_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92027.1"
                     /translation="MVSACLVLAALSYALFGGALPAGAQGQQARDLSDAAPEAAPEQW
                     IAPLPDDLKVPMWGWTARHRARWVIWPSDNYIAARQPPTDRAGLPPDEREAKRARRIE
                     PLSAARADALWWLRAMLRPEFVPDDPDAQLVLLQEEDPRESSVLSRVEANGTQVQVRQ
                     FRWALCVVIKPDPALVHGLAPEAIGPAVFRAVFSKGNQMAMADAIAVPGAPAGITMYR
                     GAAAPGRLWWQWQLWYTDGSAVGLYMVRYDGGQLTVGADDPWF"
     gene            complement(5383..>6105)
                     /locus_tag="PLQ54_01840"
     CDS             complement(5383..>6105)
                     /locus_tag="PLQ54_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92028.1"
                     /translation="PSSHTVYVVYAAPVHGVEGQRNYPTEVRLDFCMDAVSGDSDKVY
                     IANHLAALVDGWVNQVGPMEENPRWVFWARDPVDYAAGCNDQAALVCSALGVVGVEGV
                     VHQVYATCHPVPAPYDEQGGEPDMPLFTVNDYVSYYHDHRMKTRGAWPAPLQHLVFLG
                     NHFEGCFRVEDGSEDDGNMWWVVSPYARPFSTARDLLRWYADENPQLWRYEEDGYVDG
                     EPVPVPSDAFLFPRPKILGGPD"
BASE COUNT          936 a         2139 c         2116 g          914 t
ORIGIN      
        1 gggagcgtac cacgtcttgc ggagccaact gccggctctg ccggtgcccg cgggcagccg
       61 ccgcgcggtc tgcacggcgg ttgatggtac cgaacgcagc gggcggtcgg acaccaccgt
      121 gctggccgac gcggccggtc cgcccgaggg cgtgacgcac ctggcggctg atgcgtcgac
      181 accgttcgcc gttcacctca cgtggcagcc gggcgcggca ggccagcggg tgcgttacga
      241 ggtccaccgg ggcccggagg gcttcgcccc ctcggcccag acgctgctgg gagagacggc
      301 gctgacggag ttcatcgaca ccacgccgcc actggggcag cgcgtggcct acgccgtcat
      361 cccagtcgac gcggccggcc gcatggggcc cgtcagcgag tgtgtggctg aggctccatc
      421 gccgccgctt ctcccggcgg tcacgggcgt gagggccacg agcggtcccg ggcgagccgt
      481 cgtgagtttc gacccgctgc ccgatcgagg cgtggtgggg taccgtgtcc agcggcaggc
      541 cggaactgag tgggtcagcg cggccggagc cacgccgctg cgcggtgtct ccctggtcgt
      601 ggccccgctc gagccgggcc ggcccgagcg cttccgcgtt gtggccgtcg atcgggccgg
      661 gagggacggg gcgccttcgg agcccgtgac cgtgaccgca accgcggcac cgatcgagcc
      721 ggtgttcgcc acgagcttcg gcggcacgtc cgccgagact ggccagcagg gccagttgca
      781 gggtggcgcc accgtcgctg acgggtgcct cgacacgcgc gagggcggct ggatagcatt
      841 ccccaaccag gataccctcc agctaggcgg accgttgagc ttcgacctgt ggatccggcc
      901 cgcctcgctt gagggcattc ccgtgttcct ctcgtatggc cactgggagg gcctcggcta
      961 ctggctgcag ttcattggcg ggaagctccg ctactatctg ccgcggcagc agatcctcga
     1021 tgccggcagc atccccgtgg gcgagtggtc atacatagcc gccgtcttca ccgggtccga
     1081 gcagatcctc tacgtcaatg gccaggaggt tggccgccgg ggcgtcccga ccctcgacgc
     1141 ccagccgtgg ccgggcgagc tgcgcatcgg gcaatactcc gacatcgatg cccagttcca
     1201 ggcacgggcc ctcatcgacg atgtgcggat ctaccagcgt cctctgagcg ccgaggaggt
     1261 ccgggccgcg tacgaacagg ggcggcggta aagccaacac cacggggctg tgccccagtc
     1321 cccccgcaag aggccagccc ctcgcggcag ccaggcggca ggccacggag ccgcccgtca
     1381 ccgcgcccgg tagtccgcca ccacatcgtc gcacagcaga cgcccaccga cggtctccct
     1441 gtgactgccg ctggggaccg acacgagaac gagggcgcgg tcacccgcga tccgcacctt
     1501 ggcgtcgccg tgggtgccct cgaccaagaa ccggtggctg acggcgtcgt agaggtccgt
     1561 gggctccacg gcgagcggca ccgtgatcat gcgcgccgcc gcgtgcgggt tgtagtacag
     1621 gtgtgtcgga tacgcccgcg cgcggtagaa gtccgtcgcc aggcagtcga gggcgaggat
     1681 gccggggaca tcggtggtgg agacgatgcc gcccagcagg cccacgtagc ttgagccgta
     1741 gaggcccagg ttggtcttgg gcccccaccg gaactcgatc gggtcgccgg tgccgaaggg
     1801 gctgcggcca tcgcgctgcc ggcgcagccc ctcgtaggcg atggccccct cggggtcgcc
     1861 ctgccaccag ccgcagtcct ggttcgacgt gggcagctcg gtggggtaga agagccgggc
     1921 tgcgttggcc aggttcagca tccacttccc gagcgcccgg gcgtagcgcg ggtcataccg
     1981 ggccacgggg gtgagcgccg cggcctgcga gaaggtgttc atggcgaagg cgtacccgcc
     2041 acggttgtcg acgctgccga cgagcccggc gcagtcgtat ccgccccagt tctggacgat
     2101 ggcgccccag cccccgcgac agtcgctgat gtcgaagcac cagttcaaca ggcggtcgac
     2161 gtcccactcg gcgcccagct cggcgttcat cttggcagca gcctgcacgc cccacgggag
     2221 caggcactcg tagtaggggt tcgactcgcg ttcctcgagg aatcgcaggc accgctgggc
     2281 cgcgcggagg tgggccggat cgcgccagcg tgtccaggcg gcgtactcca tccaagcaat
     2341 gccggcggcg gcatcgggct cgcgccactt gccgttgtcc accggcttcc ccgacgcgaa
     2401 gtcgaagccc gtgtgctcga atgtggcatc gacggcgtcg acgactcggg cccaccggtc
     2461 ggccgtgtcg cgcaccacgg gttcgagccc cgcgtccggg taccggtccg ccagagcgta
     2521 aaagacgatg tgggggaaga ggtcatacca gtaggagccg ccggtgccct ggtcttgcaa
     2581 gttgagcacc aggttcgacc cgttgcggcg gttcgcgaag gcctggcaca tggcgacgta
     2641 gtcgtgcgga ccctctcgct tgtcgatgcc cgccacggtc gcgccgagga cggcgcccat
     2701 gcacgtgatg ctctcctggc cgttcgtgcc ctgccgcggg tcgcccacat agctcggtag
     2761 gccgaaggtc ggagcgtcta cgttgatgcg gctctcgtcg agccagatca gcggcaggaa
     2821 ctggcctcgg gcactccagt cgaaggccag gcggtcatag gcgcgcgcct tggcgcgcca
     2881 gtcgagcatc cggtagggct gtgggaggct gggcatcaga tcgacggccg cgatgggcag
     2941 ttggcccggg atggtcgccc cgcgtggaga cggggcccca gacgcgaact caaggccctc
     3001 gaagacaacg ctgtcgcccg gcttgccctc gatgccaagc tggagctgca cgggctggcc
     3061 gtgacccagg agcatcaggc gtgggtcggg ttcgaggatg gtctggcccg ttcgggcaca
     3121 ctgctcggcc agcgaggagg tcgcggggcg ccccgtgcca cggacatcgc cgaagagccg
     3181 catcagccac cggctgccct cgctcaggct gcggacgctt gcggtgacgc gttccgtacc
     3241 cggcgggagg cacgccgacg ggatggccgc aatggcccag gcgcagccgg gcgcgatctc
     3301 gacccgcaat gccccgcggt acggggtgac gagcgcgccg ttcatccggt cgctccaggc
     3361 cgacggtcgg ctgagcaggg cttcggacca ctgaagggcc ggtggggcgc cggccagcac
     3421 ggcgcacgcg cagagcgctg agaaggtcat ggcgtgcctc cccaggacgg gcatcggttc
     3481 gtccggttcg ggccgcagtt tcggctcatc ggcacggggg ccttcccact tggtgcactg
     3541 gcggggggcc tcccggcgcg gaggaccgta tccgcgccgt ggtgttcagc ggaaggagct
     3601 cctgaccccg cctcgccgtg ctgctgccgg cgccatcatc agaaccacgg gtcgccggct
     3661 ctcgccgggg agtgtcctct gtcggctctc ctgatgtaga ctgcggtaga cctgccgtcg
     3721 gtgtaccaga agaacccgcc ccaccagagt gccgagctga cggaaaggtc caactcctgc
     3781 gcgaagtagg gcagtccggg gacgtccgag aagcgcgctc ccgccatcgc gtcgcccttg
     3841 gtgaagacgg cgcggaacgc cgcggggccg atggcccgcg gctccagccc ctcgatcaag
     3901 gccgggtcgg gccgcatcac gacgcagagc gcccaccgga gctgagtgac ttggatgcct
     3961 gtcccgtgga tctcgaagcg gcagagcacc gagctccgcg acgggtcctc ctcctgcaga
     4021 agcacgaacc cgtcgtcgag gtcgtcgggg accagctctg gcttcagaat ccggcgcacc
     4081 caggctgcgg catcggctcg ggcctcatca ggggagacgg cgcctgggtc accgcgaacg
     4141 atgggcttgc cgtccgcccc ctccgccggg aactcggcgc ggcggtctcg cagcggtccc
     4201 cgggctctca cctcgtcctc cgaaggccag acgacccact tgcccatcca ctcctcgacc
     4261 cagtcttcgc ccaccgcgcg gtccacatcg tctggcaggg gcgcgatcca ctgctcgggg
     4321 gcaggctggg ccgcaggtgg gtcctcagcg gacgcagcga caaggcaggt cagaatcatg
     4381 cagagcggca ctcctagccc tgtgggacgt gtcaaagcac cggcctcctt ctcgctagcc
     4441 gcacgccgcg gccacgcgca ggcccggggt ctggatcctc cggcgcggaa gggcgcacca
     4501 gccggaggct cctgaccccg cctcggccgc agccctcagg cagcggggcg cgcactcgac
     4561 gtgccccgct ggcaacacca cgctcagaac caggggtcgt ccgctccaac agtgagctgg
     4621 ccgccatcgt acctgaccat gtacagcccc acagccgacc cgtccgtgta ccagagctgc
     4681 cattgccacc acaggcggcc cggcgcggcg gcaccgcgat acatggtgat accggccggc
     4741 gctcccggca ccgcgatggc gtccgccatc gccatctggt tgcccttgga gaacacggcg
     4801 cggaagaccg cagggccgat ggcctcgggc gccaggccat gcacgagcgc cgggtcaggc
     4861 ttgatgacga cgcacagcgc ccaccggaac tgacggacct ggacttgcgt gccgttggcc
     4921 tcgacccggc tcaggaccga agactccctg ggatcttcct cctgcagaag cacgagttgg
     4981 gcgtccgggt cgtctggtac gaactcaggc cggagcatcg ccctgagcca ccaaagggca
     5041 tcagccctag ccgctgacag aggctctatg cggcgggccc gctttgcctc ccgctcatcg
     5101 ggtggaagcc ccgctcgatc ggtgggtggc tgccgcgccg cgatgtagtt gtccgagggc
     5161 cagatgaccc agcgagcacg gtggcgcgcc gtccaccccc acatgggcac cttgagatcg
     5221 tccggcagcg gcgcgatcca ctgctcggga gcggcctccg gcgcagcgtc cgaaagatca
     5281 cgcgcttgct gcccctgcgc acctgctggc aatgctcccc cgaacagggc gtatgacaga
     5341 gccgccaaca ccaaacatgc agacaccatg tcaggcttcc ccctagtccg ggccgcccag
     5401 gatcttcggg cggggaaaca ggaaagcatc ggaagggaca ggaactggtt caccatcgac
     5461 gtatccgtcc tcctcatatc tccatagctg ggggttctcg tcagcgtacc atcggaggag
     5521 gtccctcgcg gtgctgaacg gcctggcgta gggcgatacg acccaccaca tgttgccgtc
     5581 atcctcgctg ccatcctcga cgcggaagca cccctcgaag tggttgccca gaaacaccag
     5641 atgctggagt ggggctggcc acgcgcccct ggtcttcatc ctgtggtcgt ggtagtagct
     5701 gacgtagtcg ttcaccgtga acaggggcat atcgggctcg cccccctgct catcgtaggg
     5761 ggcaggcacc gggtggcagg tcgcgtagac ctggtgcacc acgccctcta caccaaccac
     5821 tcccaaggca ctgcagacca gagcggcctg gtcgttgcac ccagcggcgt agtcgaccgg
     5881 gtctcttgcc cagaacaccc agcgtgggtt ctcctccatt gggccgacct ggttgaccca
     5941 gccatcgacc aatgccgcca ggtggttcgc gatgtagacc ttgtcactat cgccgctcac
     6001 cgcgtccatg cagaagtcga ggcggacctc cgtggggtag ttccgctgtc cctctacgcc
     6061 gtggactgga gcagcgtaga cgacgtagac cgtgtgggag ctcgg
//
LOCUS       DAOSPL010000057         6028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_13272_length_6028_cov_3.246945, whole genome shotgun sequence.
ACCESSION   DAOSPL010000057 DAOSPL010000000
VERSION     DAOSPL010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13272_length_6028_cov_3.246945"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLQ54_01845"
     CDS             <1..217
                     /locus_tag="PLQ54_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HOS92029.1"
                     /translation="SRTGTWYSFGEQRLGQGRDNAREFLEQNPAVASEIEQAVLAAVG
                     VRDARAPRSLDGVSEAPEEGPVEGDLE"
     gene            476..2032
                     /gene="rny"
                     /locus_tag="PLQ54_01850"
     CDS             476..2032
                     /gene="rny"
                     /locus_tag="PLQ54_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOS92030.1"
                     /translation="MPYIEVIVGTCIALLGLAIGGVIAVRANQRQKAYERQCRDVEKD
                     VLARAQELERSAEARRKELEIEGRQAAIELREQIEEEYRRERSELNRMERRLEQKEAA
                     LDKRVADLDAQKKQLDNREAALVQRDEELKQVLATQRQELERISGLSAQEARQILLKA
                     IEEQTRLEAAKMVRDIEEETREEATKRARHIISHAIQKCAVDQVAESTIAVVPLPNDD
                     MKGRIIGREGRNIRAFEALTGVDLIIDDTPEAVVVSGFDPVRREVARTALTALITDGR
                     IHPGRIEEIIEKAQNEVEERMREAAEGAMIATGVTGLPAEIRQLLGKLKFRTSYGQNV
                     LDHSVECAHLAGAMAAELGLNPALAKRAALLHDIGKATDYQAEGSHAEIGYEVAKRHH
                     EPPEVLNAIMAHHGEQDFTCLEAVLVQAADAISASRPGARRESLESYIRRLERLERIA
                     DTFQGVEKAFAIQAGREIRVVVKPEQIDDLQASQMARDMAKRIEEELEYPGQIRVTVI
                     RETRTVEYAK"
     gene            2177..2887
                     /locus_tag="PLQ54_01855"
     CDS             2177..2887
                     /locus_tag="PLQ54_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOS92031.1"
                     /translation="MPGLRQELSADYVVANAENAAAGMGITPNVCEALLGAADILTLG
                     NHTFRHKEIEVYLSSSERVVRPANYHPAAPGRGWALTSREGQVPLAVLSLQGTVFMES
                     MASPFTTFDSLIDELRSRTPAVIVDFHAEATAEKAALAYHADGRCSAVLGTHTHVQTA
                     DERILAGGTAFISDVGMTGPVDSIIGMDAGLVHRRFVLGLPTRFSVAAGAATLWGVLV
                     DIDGATGSATAIQRIRID"
     gene            2893..4356
                     /locus_tag="PLQ54_01860"
     CDS             2893..4356
                     /locus_tag="PLQ54_01860"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HOS92032.1"
                     /translation="MISLDPALDAMNSWLHGRRDAMAADLARLIQAPSVRGPDRGAPV
                     AGIAQALDVARELSERAGLRYRQIEDWVGYADLGEGSETVGVLLHLDVVPAGEGWTHP
                     PFSGALVDGEVWGRGAQDDKGPVISVLHAAEAVARSGAPFQRRLRVIFGTDEESGVWT
                     DIARYLECEPAPTLGFVPDGCFPITSGEKGVLNVDLVAPANRDAPARGITVTSLWAGE
                     RANIVPGSARAVLRSTASPESLAEALAHRADAYNSTHAKAAIGARVEREGVVVTAAGQ
                     IAHGSDPAGGHSALLDLVDFLDAEDLTENGPGQMVRFLSSRVGFDLCGERLGLFARHS
                     FVGCTTVNIGVVSTGLDGACRAEANIRIPVGRTISDMVSGLERATGAHNRATGHGLRV
                     DHVGAGHEPLYIEPDSELVRALSRAFEASTGQPADLKAIGGTTFAKAMPNMVAFGPGM
                     EGDPGCAHQVDERTSVAVLVRNALIYASAIRELCCVR"
     gene            4484..4957
                     /locus_tag="PLQ54_01865"
     CDS             4484..4957
                     /locus_tag="PLQ54_01865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS92033.1"
                     /translation="MRIRTLTAADAPALLRLYQAFSDEVNRYFRPFETVTLEVLRDHL
                     ERGDAGEHLTLGVAEPDGSLWGHGFVLGIRGERPVFGIGLHQDLIGKGWGRRLMEAVI
                     NAADASGAPLVTLTVLKENHRAVSLYESMGFELRGDCTFRMENDSHYMERRRTAP"
     gene            4954..>6028
                     /locus_tag="PLQ54_01870"
     CDS             4954..>6028
                     /locus_tag="PLQ54_01870"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS92034.1"
                     /translation="MTPMTFRQRLLAALNGEPTDVLPWFADLTYWYHARRAEGTLPAE
                     YEGEEGFVRLHIDHHVGYYLGYTSVFSTSYDGCRVEGTTDGDRATTRWVTPVGEILGV
                     TQYLPLTYSGAWVKWPVETPEDLRVLRYLAESTVITRDYSHYERQKAIAGDQGHPSIL
                     PPRSPVSAMLAEWSGATNLAYLLADAREEVESVLTALGSAADGAYEAIIAARPPFIEF
                     PDNLTGEVVTRWFERYQFDYYVRRIEALHGAGIRVGTHLDGTMRGILPLLVRTGLDYI
                     ESITPKPTGDVAIADLRQLAGHDVILFGGMPGALFAPPFGPDDVRRHVEEVVAHHWGS
                     NRFILGVADQLPPNGDIGLVRLVG"
BASE COUNT         1008 a         1881 c         2198 g          941 t
ORIGIN      
        1 gtcccgcacg ggcacctggt acagcttcgg cgagcagcgg ctggggcaag ggcgtgacaa
       61 tgcgagggag tttctggagc agaacccagc ggtggcatcg gagattgagc aagccgtgct
      121 ggctgccgtc ggggtgcggg acgcccgagc ccctcgctca ctcgatgggg tgtccgaggc
      181 gccggaggag ggaccggtcg agggggacct ggagtaggta cggagctaca cagatgcggg
      241 ggtgaggtaa gcgataactc gcgagtccct cgggcgcgtt cgtgaggccc cggctcgcgg
      301 gaactcggca tgaggatctg gaggatggtt ggcgcgccgg aggcgcttac gacgccgccg
      361 ggcggtcggc gatggatctc gggccggccg cgccccttgg ggaagcagcg cccactcttc
      421 cgctatccgc ggcccgtccg acaggcgggg ccgcaacagg ggggtggacc gagatttgcc
      481 atatatcgag gtgatcgtcg gaacgtgcat cgccctgctg gggttggcca tcggcggcgt
      541 cattgccgtc cgggcgaacc agcgccagaa ggcgtatgaa cgacagtgcc gcgatgtgga
      601 aaaggatgtg ctggcgcgcg cccaggagct cgagaggtcg gccgaggccc gccgcaagga
      661 gctcgagatt gaggggcgcc aggccgccat cgagttgcgg gagcagatcg aggaggagta
      721 ccgccgcgag cgttcggagc tgaaccggat ggaacgccgc ctggagcaga aagaggcggc
      781 gctcgacaag cgcgtggcgg atctggacgc gcagaagaag cagctggaca accgggaagc
      841 cgcgctggtg cagcgcgacg aggagctcaa gcaggttctg gcaacccagc ggcaggagct
      901 ggagcgcatc tcgggcttgt cggcccagga ggcgcggcag atcctgctga aagccatcga
      961 ggagcagacg cgcctcgagg cagccaagat ggtgcgagac atcgaagagg agacacgcga
     1021 agaggccacc aagagggccc gtcacatcat cagtcacgcc attcagaagt gcgcggtcga
     1081 ccaggtagca gagagcacca tcgccgttgt gcccctgccg aacgacgata tgaaggggcg
     1141 aatcatcggt cgggagggcc ggaacatccg agccttcgag gcacttaccg gagtcgacct
     1201 gatcatcgac gacacaccgg aggcggtggt ggtctccggg ttcgacccgg tgcgacgcga
     1261 ggtcgcgcgg accgcactga ccgcgctcat caccgacgga cgcatccatc cggggcgtat
     1321 cgaagagatc attgagaagg cgcagaacga ggtcgaggag cgcatgcggg aggcggcgga
     1381 aggggccatg atcgccacgg gcgtcacggg cctgcctgcc gagatccggc agctcctcgg
     1441 gaagctcaag ttccggacca gctacgggca gaacgtgctg gaccacagcg tcgagtgtgc
     1501 ccacttggcc ggggccatgg cggccgagct cgggttgaac cccgcgctgg caaagcgggc
     1561 ggctctgctg catgacatcg gcaaagccac ggactaccag gctgagggaa gccatgcgga
     1621 gatcgggtac gaggtggcga aacgccacca cgaaccaccc gaagtgctga atgccatcat
     1681 ggcgcaccac ggcgagcagg atttcacctg cctggaggcc gtgttggttc aggcggccga
     1741 cgccatctcg gcatctcggc cgggcgctcg gcgcgagagc ctggagagct acatccggcg
     1801 cctcgagcgc ctggagcgca ttgcggacac gttccaggga gtggagaagg cctttgccat
     1861 tcaggcgggc cgcgagatcc gcgtggtggt gaagccggag cagatcgacg atctgcaggc
     1921 aagccagatg gctcgcgaca tggccaagcg gatcgaggag gaactggagt accccggcca
     1981 gatccgggtc acggtgatcc gggagacacg aaccgtggag tacgccaagt gagagggtac
     2041 cgcaccgggc cgtcgcgatg gccgcggtct gccagctgga ctgcggtccg ggcggcccgg
     2101 gggcgggctt gccgatgagg gtcctgttcg tgggcgacat cgtgggcggc cccgggcgcg
     2161 aggtcgtctt ggctcggtgc ccggtctgcg gcaggagctg tcggcggatt acgtggtggc
     2221 caacgccgag aacgccgcgg cgggcatggg gatcaccccg aacgtctgcg aggcccttct
     2281 gggcgcggcg gacatcctga cactgggtaa tcacacgttc cggcataagg agattgaagt
     2341 ttaccttagc tcgtccgagc gtgtggtgcg ccctgccaac taccacccgg cggccccggg
     2401 gcgcgggtgg gcgctgacct cgcgcgaggg gcaggttccg ctggccgtgc tcagcctcca
     2461 gggcacggtg ttcatggaga gcatggcgtc gccgttcacg acgtttgact ctctgatcga
     2521 cgagctccga tctcgcacgc ctgccgtgat tgtggacttc cacgcagagg ccacggcgga
     2581 gaaggcggcg ttggcctacc acgctgacgg gcgctgctca gccgtgctcg gtacccacac
     2641 ccacgtgcaa acggccgatg aacgcatcct ggcgggcggg acggccttca tcagcgacgt
     2701 ggggatgacc ggcccggtcg actccatcat cgggatggac gcggggctcg tgcaccgtcg
     2761 tttcgtgctg ggtctgccca cgcggttctc agtggcggcc ggggccgcca cgctctgggg
     2821 cgtgctggtc gacatcgatg gtgccaccgg aagcgccacc gccatccagc ggattcgtat
     2881 cgactagccg aggtgatctc actggacccc gctctcgatg ccatgaactc ctggctccat
     2941 ggccggcgtg acgccatggc cgccgacctg gctcggctga tccaggcccc gtctgtgcgc
     3001 ggccccgatc gtggggcgcc ggtcgcgggc atcgcccagg ccctggatgt ggcgcgcgag
     3061 ctgtcggaac gtgcgggact ccggtaccgg cagatcgagg actgggtggg ctacgccgac
     3121 ctgggcgagg gaagtgagac ggttggggtc ctgctccatc tcgacgtggt accggcaggc
     3181 gagggctgga cgcatcctcc gttctcgggc gcgctcgtcg acggtgaggt atggggacgt
     3241 ggggcccagg atgacaaggg accggtcatc tcggtcctgc acgcggccga ggcggtcgct
     3301 cggtctggtg cgccattcca gcgccgcctg cgcgtgatct tcggaaccga cgaggagtct
     3361 ggtgtctgga cggacattgc gcggtatctg gagtgcgagc cggcgccgac gctgggcttc
     3421 gtgcccgatg gatgcttccc catcaccagt ggcgagaagg gcgtgctcaa cgtggacctg
     3481 gtggctccag ccaaccgcga cgcgccagcg cgtgggatca cggtcacgtc gctctgggcg
     3541 ggggagcgag ccaatatcgt gccggggagc gcccgtgctg ttctgaggtc gaccgcgtca
     3601 cccgagagtc tcgccgaggc cctcgctcat cgggcggacg cgtacaactc gacgcacgcc
     3661 aaggccgcga tcggtgcccg ggtggagcgg gaaggcgtgg tcgtcacggc ggcggggcag
     3721 attgcgcacg ggagcgatcc cgccgggggc cacagcgcgt tgctggacct ggtggacttc
     3781 ctcgacgccg aggacctcac cgagaacggg cccggccaga tggtccgctt cctgtcgtca
     3841 cgcgtggggt tcgatctgtg cggtgagcgg ctcgggctct tcgcccgcca cagcttcgtc
     3901 ggctgcacga ccgtgaacat cggggtagtc tccaccggac tcgacggtgc gtgccgggcc
     3961 gaagcgaata tccgcatccc ggtgggccgg accatctccg acatggtgag tgggctcgaa
     4021 cgagcgacgg gggcccataa tcgtgcgacc gggcatggac tgcgcgtcga tcatgtgggt
     4081 gcgggccacg agcccctgta catcgagccg gactcggagc tggtgcgggc gctcagccgg
     4141 gccttcgagg cttcgaccgg gcagccggcg gatctgaagg ccatcggcgg caccaccttc
     4201 gccaaagcca tgccgaacat ggttgccttc ggtccgggta tggagggcga tccggggtgc
     4261 gcgcaccagg tcgatgagcg gacgagtgtt gccgtgctgg tccgcaacgc gctgatctac
     4321 gcctcagcca tccgcgagtt gtgctgcgtg cgttaggccg cacgccttgc agtggttcac
     4381 gcctgcggtg gcgcagaaac cacctcacag tacgtctgcg cggaaggact tcccacgccc
     4441 tcacgggaac gtcggttcgc aacgtgcagg ttgggagcgc gccatgcgca ttcgcacact
     4501 gacggccgcc gacgcccccg cgctgctgcg gctctaccag gccttctccg atgaggtcaa
     4561 caggtacttc cgccccttcg agacagtgac actcgaggtg ttacgtgatc acctcgagcg
     4621 gggcgacgcc ggcgagcatc tgaccctggg tgtggccgag cccgacggct cactgtgggg
     4681 ccacgggttc gtgctgggca tccgcggcga gagaccggtg tttggcatcg ggctgcatca
     4741 ggacctgatc gggaaaggat ggggccggag gctcatggag gcggtgatca atgcggccga
     4801 cgccagcggc gcgccgctgg tgaccctcac ggtactcaag gagaaccatc gagccgtgtc
     4861 gttgtatgag tcaatggggt tcgagctgcg cggcgactgc acgttccgga tggagaatga
     4921 cagccactac atggagcgga ggaggaccgc gccatgacgc ccatgacgtt ccggcagcgg
     4981 ctcctggccg cgctcaacgg cgagcccacc gatgtgctgc cgtggttcgc ggacctgacc
     5041 tactggtacc atgcgcggag agcggagggc acgctgccgg ccgagtacga gggcgaggag
     5101 gggttcgttc gcctccacat cgaccaccac gtcgggtact acctcgggta cacctcggtc
     5161 ttcagcacca gctacgacgg ctgccgggtc gagggcacta ccgacggcga tcgcgcaacc
     5221 acgcggtggg ttacgcccgt cggcgagata ctcggagtca cgcaatacct gcccctgacc
     5281 tacagcggcg cctgggtgaa gtggccagtc gagacgcccg aggatctgcg cgtcctgcgg
     5341 tacctggctg agtcgacggt catcacgcgc gactacagcc attacgagcg ccagaaggcc
     5401 atcgcgggcg accaggggca cccatcgatc cttccgccgc gctctcccgt ctcggccatg
     5461 ctggccgaat ggagcggggc cacgaatcta gcctacctgc tcgccgatgc ccgcgaggag
     5521 gtcgagtcgg tcctgactgc cctgggcagt gcggcggacg gagcgtatga ggccatcatc
     5581 gccgcgcggc cgccgttcat cgagttcccg gacaacctga ccggcgaagt ggtcactcgc
     5641 tggtttgagc gctaccagtt cgactactac gtgcgccgga ttgaggcact gcacggcgcg
     5701 ggcatccggg tgggcaccca tctcgacggt accatgcgcg gcattctgcc gctgctggtg
     5761 cgcacggggc tcgactatat cgagtccatc acgcccaagc ccacgggcga tgtggccatc
     5821 gccgatctgc gacaactcgc cggccacgat gtcattctgt tcggtggtat gccgggggcg
     5881 ctgtttgcgc cgcctttcgg gccggatgac gtgcggcggc acgtcgaaga ggtggtcgcg
     5941 caccattggg gctcgaacag gttcatcctg ggagtggccg accagctacc gcccaacggt
     6001 gacattgggc tcgtgcgtct cgtgggtg
//
LOCUS       DAOSPL010000058         6013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_13323_length_6013_cov_3.462068, whole genome shotgun sequence.
ACCESSION   DAOSPL010000058 DAOSPL010000000
VERSION     DAOSPL010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13323_length_6013_cov_3.462068"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..829
                     /locus_tag="PLQ54_01875"
     CDS             65..829
                     /locus_tag="PLQ54_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92035.1"
                     /translation="MPDRDLGHCERYWGMREPAFSLAPDPRFLHLSGQHQDALNALTL
                     AVTSLRPLAVLVGPNGCGKTSLARKILRELPPEDYRAVTCTAHGPLVCPHLFAASGWD
                     RGPDETASEPAGTSRPIVAVVDEAQAAPRSALAAWLAEASTHPRLTVILVGQPGLWEP
                     PPGHFDAIVCAVGPLSRPDTDAMIRHRLRTAWYSGPEPFTDEALSELHHHSAGVPRLA
                     CELAANALAQACAQGSREVDAPIVAAVAAELLAPSA"
     gene            889..1269
                     /locus_tag="PLQ54_01880"
     CDS             889..1269
                     /locus_tag="PLQ54_01880"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOS92036.1"
                     /translation="MSGSDRRRNPRREDRSAIYYRLVDATGPGPSRLRGAVSGNLSAG
                     GLLLLTRHEHGPGTRLELELLLRAHSGSPVCHHVVFAGEVVRCEPQPGSDARFELGVR
                     MDALSDEDRDRLAAYLAELERDTA"
     gene            1333..2508
                     /gene="pilM"
                     /locus_tag="PLQ54_01885"
     CDS             1333..2508
                     /gene="pilM"
                     /locus_tag="PLQ54_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOS92037.1"
                     /translation="MGLFGGAKRELIGVDIGSYAIKAAYVAPGRSGPELVALASIRTP
                     PGAIEDGIVTDVAATAAALRELLDAAGLTVRDAATAVGGPRVIARPQTFPHMSEARLR
                     QTALYEAEKFIAFSLEESIVEPQIVSERQTEDGRMLDVMVVAAQTQLVDSRVQVLQRA
                     GLSPAFVDIEAFALLRSMIYGTHDPDVLQSNVGIVRLGESYGDITIVASGSYVLYRTL
                     PGAGRALTEAIAAQLGVTYHEAEAIKETEAVAVTPDVAGALNERSARVSEAIQPVLDD
                     LTYELRLSLGFFQAQFQDVSEGGTVRHLLLTGGTSVMRNLDRYLAAAVGVPVSRRQFI
                     DSLALDRGRFDPAYLAAASPLAGVAVGLTLTNHMKTGSYAFAADPSQFGVALQRRQQ"
     gene            2556..3137
                     /locus_tag="PLQ54_01890"
     CDS             2556..3137
                     /locus_tag="PLQ54_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92038.1"
                     /translation="MLHVNLIGARQRERQAQSRVTAECVLAGFVVLCLALWLAGPKVA
                     RRYEVLRSIARYEKQIADNEARADRVQRMKDESEQLAPVFTMVRESQYCVVDWLRVLM
                     HIQEEMPRGVWAEQVASEFKQEDWSHKVKVHGKAARQADVAEAMVRIGRRTPFDEKRM
                     TLLQTNVSKTSDEEYIEFSFDAGLNYVIGEYLQ"
     gene            3134..3997
                     /locus_tag="PLQ54_01895"
     CDS             3134..3997
                     /locus_tag="PLQ54_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92039.1"
                     /translation="MNKRGMNPKSKLAIIITVLACVVVLAVALFLRAQSIAAVARSNK
                     TLEETKQRAEEVASLVASESSEAATLERLRSETAFVSRLARDAKQAQELAQQGVPNVI
                     LQADFVPAVLRDLEVLGRRTGVEVRSVKPGIYKKLEIRKKPQDEVPATDEEARKKAEE
                     AWNKMSAEEKRPIEKDHEYDKVYKVLTRSASLLSMDIEVAGQYSALFEFIKGLAYPAA
                     DGWRLRELVAVRNIRLEAERDPHAKDVEPNVRAYLSAVAFAVRPPTPKADAEKKDSGT
                     SSDGGGGGEGK"
     gene            3984..5069
                     /locus_tag="PLQ54_01900"
     CDS             3984..5069
                     /locus_tag="PLQ54_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92040.1"
                     /translation="MRASKAQLAIVAVLMVVLAGSLYMLLFRKPAAAPAKTAVTPASA
                     DAKGTQREPAKAARPPGEARKATAPATAEKPAPTASAQGTPSAPVTKTDEKCPPGVQP
                     GLWAAWGTYSKQRPSQAAAASAPGTELAAIAEGTSYTGMDPDEIDKARQAEKAGPLPD
                     GRAFRLRQGPSPFKRPSVTQLAALRGQAPSGEAPPASERAETATAQTPPATASAGAEK
                     PATAAAPSAPPSAPPAAIASGLPPAIGATAPGVPLPDVGAPATVQPQPQRPAFLGGQG
                     FPQATTGPRSGAKPALNLRVTGTVVASDGRSSAVITDGTNTFYVRAGQTFRAGGREFR
                     VVSVTQGAVEVENQQRRLTLGIGGAAP"
     gene            5210..>6013
                     /locus_tag="PLQ54_01905"
     CDS             5210..>6013
                     /locus_tag="PLQ54_01905"
                     /inference="COORDINATES: protein motif:HMM:NF023169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HOS92041.1"
                     /translation="MIGVRVHADTGGARITFFAATPIRYEAKRLTNPTRLLMQFPGAV
                     FDRDQASRPTIVGMGALAKVLLGQDSKNPSVANAVLYLRARPRVDIARSDDGCTLVVT
                     FDSPRALPDETAPASPNTPAEIGVAELIRRATGEPIAIRLLADRAVRYEAKSSGDDVI
                     LDIQGAVLAPGLPTSLPVNAGGIGSVALAQPSRDTVRATLDLDGAGTYHVAPIENGCG
                     VLVSLMPPAAPASLDLAPAPHAGGTAPPAPLGPYGMNQAGFGGSPTTPAP"
BASE COUNT         1001 a         2144 c         1981 g          887 t
ORIGIN      
        1 ggccggtttg cattacaatg ttcacacgtc tgctgcccca tgcgcgcgag acgggtgatc
       61 ggcggtgccg gatagagacc tgggacactg cgagcggtac tgggggatgc gcgagcccgc
      121 cttctctctg gcgcccgacc cgcgctttct acacctgtcc ggccaacacc aggacgcgct
      181 gaacgccctc acccttgccg tgaccagcct gcggccactg gccgttctgg tcggccccaa
      241 tggctgtggc aagacctccc tcgcccgaaa gatcctacgc gagctccctc ccgaggacta
      301 ccgggcagtg acctgcacgg cacacggacc gctcgtttgc ccgcacctgt ttgccgcttc
      361 cggctgggac cgcggcccgg acgagacggc atccgagccc gccgggacta gccggcccat
      421 cgtggccgtg gtggacgagg cccaggccgc gccccggagt gccctggctg cctggctggc
      481 cgaggcgtcg acacaccccc ggctgaccgt catattggtc ggccagccag gactgtggga
      541 gcctccgccc gggcacttcg acgccatcgt gtgcgccgtc ggccctctga gccgcccgga
      601 caccgacgcc atgatccgcc accgcctgcg tacggcctgg tactccgggc ctgagccgtt
      661 caccgatgag gccctgtccg agctgcacca ccactcggcg ggtgtgcccc ggctggcgtg
      721 tgagctggcc gccaacgcgc tggcgcaggc atgtgcccag gggtcgcgtg aggtggacgc
      781 tccgattgtg gccgccgtgg ccgccgagct gttggccccc tcggcctgac cgtcgcacgc
      841 gccgccggtg aactccgttc gacgccgccc cgttagggag gcgagccgat gtccgggtca
      901 gaccgcagac gcaacccaag gcgtgaggac cgctccgcca tctactaccg cctggtggat
      961 gccaccgggc caggtccctc ccgtctccgc ggcgcggtca gtggcaacct gagtgccgga
     1021 ggcctcctct tgctgacgcg gcatgagcat ggccccggta cgcggctgga gctcgaacta
     1081 ctgctccggg ctcacagtgg atcgccggtc tgccatcacg tggtgttcgc tggcgaagtc
     1141 gtccgctgtg agccacagcc cggctcggac gcacgcttcg agctcggtgt gcgcatggac
     1201 gcgttgagcg acgaggaccg cgaccgcctt gccgcttacc tcgccgagct ggagcgtgac
     1261 acggcatagc gcgggccgtg gccgcaccgg cggagtggcg gtcgcgggtc gggaatgcga
     1321 gggggagccc ccatgggtct gttcggcggc gccaagcgcg aactgatcgg ggtcgacatc
     1381 ggcagctacg cgatcaaggc ggcatacgtc gctccgggcc gctccgggcc cgagctcgtc
     1441 gccctcgcct cgatccgaac gccgccgggg gccatcgagg atggcatcgt caccgatgtg
     1501 gcagcgaccg ccgcggccct tcgcgagctg ctcgatgccg ccggactgac cgtccgcgac
     1561 gccgccacgg cggtgggcgg accccgggtc atcgcgcgcc cccagacctt cccgcacatg
     1621 agcgaggcgc gcctaaggca gaccgcgctc tatgaggccg agaagttcat cgcattctcc
     1681 ctcgaagaga gcatcgtcga gccccagatt gtctccgagc gccagaccga agacggccgc
     1741 atgctcgatg tcatggtcgt ggcggcccag acccagctcg tcgactcccg cgttcaggtg
     1801 ctccagcgcg cggggctgtc acccgccttc gtcgacatcg aggcgttcgc gctcctgcgg
     1861 tccatgatct acggcaccca cgaccccgac gtcttgcagt cgaacgtggg tatcgtgcgc
     1921 ctgggcgaga gctacggcga tatcaccatc gtcgcctccg gaagctacgt gctctatcgc
     1981 actctcccgg gcgccggacg ggctctcacc gaggccatcg ccgcgcaact cggagtgacc
     2041 tatcacgagg cagaagccat caaagagacg gaggccgtcg ccgttacccc cgatgtcgcc
     2101 ggggcgctga acgagcggtc ggcccgcgtc tccgaagcga tccagccggt tttggacgac
     2161 ctcacctatg agctacgcct atcgctgggc ttcttccagg cccagttcca ggatgtgtca
     2221 gaaggcggca cggtccgcca cctgctgctc accggcggta cgtccgtgat gcgcaacctg
     2281 gaccgctatc tggccgccgc cgtaggcgtg ccggtgtcgc ggcggcagtt catcgactcg
     2341 ctggccttgg accgcggccg gttcgatccg gcatacctgg cagcggcttc ccccttggcc
     2401 ggagtggctg tgggactgac gctcacgaac catatgaaga ctggatccta cgcattcgcc
     2461 gccgatccca gccagttcgg ggtcgcgctg cagcggcgcc agcagtagga cccgcgcgga
     2521 tccgggagcg ccgtgggcgc ggaggtggtc gcctcatgct tcatgtgaac ctgatcgggg
     2581 cccgtcagcg tgaacgccaa gcgcaatcga gggtcaccgc cgagtgcgtg ctcgcgggct
     2641 tcgtggttct ctgcctcgcc ttgtggctgg cgggtcccaa agtcgccagg cggtacgaag
     2701 tcctcagaag catcgcccgg tacgagaagc agatcgcgga caacgaagcc cgggccgacc
     2761 gggtccagcg catgaaggac gaatcggagc agctagcccc cgtgttcacc atggtccggg
     2821 agagccagta ctgcgtggtc gactggctcc gagtgctcat gcacatccag gaagagatgc
     2881 cgcgcggcgt ctgggccgag caggtcgcct cggagttcaa gcaagaggac tggtcgcaca
     2941 aagtcaaagt ccacggcaag gcggcacgac aggccgacgt ggccgaagcc atggtccgga
     3001 tcggccgccg gacgcccttc gacgagaaac gcatgaccct actgcagacc aacgtgtcga
     3061 agacgtcgga cgaggagtac atcgagttca gctttgacgc agggctgaac tatgtgatcg
     3121 gggagtacct acagtgaaca agcgtgggat gaacccgaaa agcaaactcg ccatcatcat
     3181 caccgtgctg gcgtgtgtcg tggtcctcgc cgtcgccctg ttcctgcgcg cccagtccat
     3241 cgccgcggtg gctcgctcca acaagacgct cgaggaaacc aagcaacggg ccgaagaggt
     3301 cgccagcctg gtggccagcg agtccagtga ggccgcgacc ctcgaacgcc tgcgctccga
     3361 gaccgccttc gtctcccgcc tggctcgcga cgccaagcag gcccaggagc tggcacaaca
     3421 aggcgtgccc aacgtcatcc ttcaggccga cttcgtgccc gccgtgctgc gcgacctcga
     3481 agtcctcggg cggcgtaccg gcgtcgaggt gaggtcggtc aagcccggca tctacaagaa
     3541 gctcgagatc cgaaagaaac cccaagacga ggtcccggcc accgatgaag aggcccgcaa
     3601 gaaggccgag gaggcctgga acaagatgtc ggccgaggag aagcggccga tcgagaaaga
     3661 tcatgaatac gataaagtct acaaggttct cacgagaagt gccagcctgc tgtcgatgga
     3721 catcgaggtg gcgggtcagt acagcgccct cttcgagttc atcaaaggac tggcctatcc
     3781 tgcggccgat ggctggcggc tccgcgagtt ggtggctgtc cggaatatcc ggctggaggc
     3841 ggaacgagac ccccatgcca aagacgtgga acccaacgtc cgcgcctatc tgagtgccgt
     3901 ggccttcgct gtccgcccgc ccacacccaa ggcggacgcg gagaagaagg actcggggac
     3961 ctcgagcgat ggaggtggcg gcggtgaggg caagtaaggc ccagctcgct atcgtggccg
     4021 tgctcatggt tgtcctggcg gggagtctct acatgctcct gttccgcaaa cctgccgcgg
     4081 cgccggcgaa gacggcagta actccggcca gcgctgacgc caaaggcacc cagcgggaac
     4141 cagccaaggc agcccggccg ccgggagaag cacggaaagc gacggcaccg gcaacggcgg
     4201 agaaacctgc ccccacggca tctgcccagg gcacaccctc cgcacccgtg accaagacag
     4261 acgagaaatg cccacccggc gtgcagccag ggctctgggc cgcctggggc acctacagca
     4321 agcagcgccc ctcgcaggcc gccgcggcgt ccgcacccgg cacggagctc gccgccatag
     4381 cggagggcac ctcctacacg gggatggatc ccgacgagat cgacaaggcc cggcaggccg
     4441 agaaagccgg cccgctgccc gacggtcgcg ccttccggct gcgacagggc ccgagcccgt
     4501 tcaagcgtcc ctctgtgacg cagctcgccg ccctccgcgg acaggcgcca tcgggtgagg
     4561 cgccaccagc cagtgaacgc gccgagacgg ccacggccca gacaccgccc gccactgcct
     4621 cggcgggtgc agagaagccg gccacggcgg cggccccgtc ggcgccacca tcggccccgc
     4681 cagccgccat cgccagtggc ctgccaccgg ccatcggggc gacggcaccc ggcgtcccac
     4741 tgcccgacgt cggggcgccc gccaccgtgc agccccagcc ccagcgaccc gccttcctgg
     4801 gcgggcaggg gttcccgcag gccacaaccg ggccccggtc cggcgccaag ccggcgctga
     4861 acctgagggt caccggcacc gttgtcgcta gcgacggccg ctcctcggca gtgatcaccg
     4921 atggcaccaa cacgttctac gtacgtgcgg gtcagacgtt ccgagcaggc gggcgcgagt
     4981 ttcgcgtcgt gtccgtcaca cagggagccg tcgaggtcga gaaccagcag cgacggctaa
     5041 cccttgggat tggaggagcc gccccgtgag cttgcgaccc tgcagaaaca cctcgatgat
     5101 tgctgcggtt gctctcgtcg gggccctggc tgcagcgagc tcggtgtggg ggtccactcc
     5161 gagccttggt tcgcagagca actcaccggc atcacccttg gagcgcggcg tgattggcgt
     5221 ccgcgtgcac gccgataccg gaggggctcg cattaccttc ttcgccgcga cgcccatcag
     5281 gtatgaggcg aagcggctca ccaacccgac gcgcctgctg atgcagttcc caggcgctgt
     5341 cttcgaccgc gatcaggcat cgcggccgac catcgtcggc atgggagcac tggccaaggt
     5401 tctgctcggc caggacagca agaacccttc cgtggccaac gcggtcctgt acctccgtgc
     5461 acggcctcgg gtcgacattg cgcgctcgga cgatgggtgc acactcgttg tgaccttcga
     5521 ctcgccacga gccctcccgg atgagacggc gcccgccagc ccgaacaccc cggccgagat
     5581 tggcgtcgcc gagctgatcc gccgtgccac cggcgagccc atcgccatcc gccttctggc
     5641 agatcgcgcg gtgcgttacg aggcgaagtc cagcggcgac gacgtgatcc tcgacatcca
     5701 gggggccgtg ctggcccctg ggcttcccac ctcgcttccc gtcaatgctg gcggcatcgg
     5761 atccgtcgcc ttggcccagc cctcgcgcga caccgtgcgc gccaccctcg acctcgacgg
     5821 ggcgggaacc taccatgtcg caccgatcga gaacggctgc ggcgtcctgg tgagcctcat
     5881 gccccccgcc gcaccggcgt ccctggatct cgcgccggca ccacacgcgg gtggcacggc
     5941 gccgccagcg cccctgggcc cctacggtat gaaccaggcc gggtttggtg gcagcccaac
     6001 caccccggca ccc
//
LOCUS       DAOSPL010000059         6009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_13336_length_6009_cov_3.179543, whole genome shotgun sequence.
ACCESSION   DAOSPL010000059 DAOSPL010000000
VERSION     DAOSPL010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13336_length_6009_cov_3.179543"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PLQ54_01910"
     CDS             complement(<1..750)
                     /locus_tag="PLQ54_01910"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HOS92042.1"
                     /translation="MRFIHTADLHIDSPLLGLERYEGAPVERMRSATRQAFRNVIELC
                     LDSRAPLLLLAGDVFDEDWRDYNTGLFFIGELNRLRDVGTQVLMVRGNHDAASEVSRH
                     LTLPPHARLLSAEEAETVVLDDLGVAVHGVSYSRREVTESLVPLFPPPVDGLTNVGLL
                     HANVGAASGHGNYSPCRLDELAALGYDYWALGHVHERQVLSAEPCIAYSGVTQGRHAR
                     EPGPRGCLLVRASDSGLAVEPFDTDVVRWAEL"
     gene            945..1769
                     /locus_tag="PLQ54_01915"
                     /pseudo
     CDS             945..1769
                     /locus_tag="PLQ54_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
     gene            1801..2058
                     /locus_tag="PLQ54_01920"
     CDS             1801..2058
                     /locus_tag="PLQ54_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HOS92043.1"
                     /translation="MGERIEVRAPDHETECTAVLGYWYKRAGDRVEAGDDLLDYDTDK
                     ATITLEAPCAGVLAEVLVPEDTPISPGQVLGTIEAAGGEPV"
     gene            2055..3290
                     /locus_tag="PLQ54_01925"
     CDS             2055..3290
                     /locus_tag="PLQ54_01925"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOS92044.1"
                     /translation="MSWSLVARDTIDAGDDVIVLADDPAPIALAAAEAAGARGSVRVA
                     ACTAEAERHLSAVLAGKATVFRAAPGDLSTDVVRAEAQLAATPPRSLAEARDVIEEAR
                     AHAQANPIIPAESADTVIAIGVLGRVPPAERRHAIEELYRVLRRGGTCVLYDAVSDEP
                     LPARLAADPAMPAVMQELELLSAMEDAKFHGLTIEHWPDEPDAVFEGIECRRIAIMAH
                     KGKEGICRDGLQAVVYLGPFKVVEDDDGHVIRRGQRFAVCKKTFGVYQRPPYLGMFAN
                     LEPPEEVPDEQAPLWDPAHPSTRSPSETKGLTRSAAAVPPPAAGATGEAYAARAVWLT
                     PDGRVRRQARVTHRYRGYFESYEEAESTARTVFEDASRYEVYRISMQEALEHMAKTGR
                     QAACPVTKGPGGGRDGARR"
     gene            3451..4203
                     /locus_tag="PLQ54_01930"
     CDS             3451..4203
                     /locus_tag="PLQ54_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOS92045.1"
                     /translation="MARIPIALQLYSIRDDCAKDFPGCLEAVAKMGYDGVEFAGYHGL
                     SAKELKKICGDLGLVPVGAHVGMDTLMGDQLQATIDFHLELGSKYPVVPWIPEEYRPD
                     ADGWRRTADMFNEIAAHLKPHGLKTGYHNHHIEWTPVGGEIPWDVFFSNTCADVIMQL
                     DLGNALHGGTHAEPYIGKYPGRSTTLHLKEFSATNDQALIGEGDVRWEEAFRLAETVG
                     GTEWYIVEQESYAFPPLECVARCLENLRKMGK"
     gene            4247..5137
                     /locus_tag="PLQ54_01935"
     CDS             4247..5137
                     /locus_tag="PLQ54_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOS92046.1"
                     /translation="MGDHDQPQSTRRAFLRDALVAGTSIGLLGAAKQVLAAGPCATRC
                     RRRIPIAVQLYSVRDDCAKDLPGVLKAVSQMGYEGVEFAGYYGRSAQELRTMLDDNGL
                     RVAGSHVGLETLLGDELEKSIEFHKTIGNKFLIVPGLGGQYTADKAAWERTAGIFNEI
                     AAKLQPLGMYTGYHNHTMEFQKFPDGTLPWDAFFGNTRQRVVMQFDTGNAMIGGASAL
                     PFLEKYPGRALTVHMKEFSPTNGEALVGEGNIPWRDIIRVCRTTGGTEWFIVEQESYA
                     YPPIECIRRCLVNLRMLVSL"
     gene            complement(5325..>6009)
                     /locus_tag="PLQ54_01940"
     CDS             complement(5325..>6009)
                     /locus_tag="PLQ54_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92047.1"
                     /translation="PGGFDLFTPFLVRAAQVVLALVATGLTAATVVYVARQRNDGPSR
                     AGAGPHVALALIMGLLFVGALLKAPLPSPAPPPEERKQALDEPPASPSEPQAAGGALA
                     GATEVPPTQKDGGPAGESRALAPLAGPSKAAPAAASVRGAEAPRPSASPKAAPAPAPL
                     PKAGGARSATSLGSGSSAAPSVKPAAESSRRPTPAPARNGGGVPAPRGGTVRSVGDEA
                     ALDQVIPTQ"
BASE COUNT         1006 a         2029 c         2023 g          951 t
ORIGIN      
        1 gagctcggcc cagcgcacaa cgtcggtgtc gaagggctcc acggcgaggc cggaatcaga
       61 cgcgcggacc agcagacacc ctctcggccc cggctcgcgg gcgtggcggc cctgggtcac
      121 accggagtag gcaatgcatg gctcagccga caacacctgg cgctcgtgca catggcccag
      181 cgcccagtag tcatagccca gtgcggcgag ttcgtccagc cggcacggcg agtagttgcc
      241 gtgccccgaa gcggcaccca cattggcgtg gaggagaccg acgttggtca gcccgtcgac
      301 cggtgggggg aacaggggaa cgaggctctc ggtcacctcg cggcgggagt agctcacgcc
      361 atggaccgcc acgccgaggt catcgagcac cacggtctcg gcctcctcgg ccgacaggag
      421 acgggcatga ggtggaagcg tcaggtggcg tgacacctcg ctggccgcgt cgtggttccc
      481 ccgtaccatc agcacctgcg ttcccacatc gcgcaaccgg ttcagctcgc caatgaagaa
      541 gagccccgtg ttgtagtcgc gccagtcttc gtcgaagacg tccccggcga gcagcaggag
      601 gggcgcacgg gagtcgaggc acaactcaat gacgttgcga aacgcctgcc gcgtcgccga
      661 tcgcatgcgc tcgacgggcg ccccctcgta gcgctcaagg cccagaagcg ggctatcaat
      721 gtgcagatca gctgtgtgga tgaaccgcat tcgccagtcc cctccgcggg ttaccccggg
      781 gagtgttgca ccagagcacg tcaggatcct cctgttcggg gcgaccgcag cacagcgccg
      841 cagagtcctg ccgacgcttt atacagtcgg atattctcag tagcatggga atatcggagc
      901 gtccctcgac ggctgggacg gacctcatcc ctcgcaggtg acacatgagg gagatccgct
      961 tggcccggca gtgcatcgcc gaggctctgg gcacgttcat ccttgtcttc ttcggcgtcg
     1021 ggtccgtcca tgcagccgtc gcgtgcggcg ctcagtaagg ggtctggcag gtggccgtgg
     1081 tctggggggt ggccacttac gccacgggcg ccctgagcgg ggcccacatg aacccggccg
     1141 tcagcatcgc catggccgcc taccgaggct ttccgtggcg caaggttccc gcttttgtgg
     1201 cgtcccagct cctgggagcc atcgtgggag cccttctgct gtacagcctc ttctgcggct
     1261 tcatcgccca gtacgaatcc gacaagggca tcacgcgcgg cgccccgggc agtgagctct
     1321 cggccatgat gtacggggag tacttcccga acccggccac ggccggcacg acgccagaag
     1381 cctatgccac ggtctctcgt cttcaggcgt tcgctggcga gatggtcggc accgcgtttc
     1441 tggccttctt catcttcgcc ttggtggaca cccgcaatcc tagccgtccg atgggtacta
     1501 gcgtggccac gttcatcggc ctgacggtct cgatcgtgat ctcgattgtg gctcccctca
     1561 cgcaggccgg cctgaacccg gctcgtgact tcgggcctcg actcgtctca tacttcctcg
     1621 ggtggggtcc catcgccata ccgggcgccg ggggcgggtt tctcgatgtc tacatcgtcg
     1681 cgcccatcgt cagagccatg gtcggggcgg gtgcctatga gctcctgatc cggccgggtc
     1741 tgcccgagag tggactccaa gcccagtgac ccataccacg cgaatggagt gacgctcgac
     1801 atgggagagc gcatcgaggt gcgagccccc gaccacgaga cggagtgcac ggcggtgctc
     1861 gggtattggt acaagcgcgc cggcgaccgg gtggaagccg gtgacgacct actcgactat
     1921 gacacggaca aggcgacgat cacgctggag gccccgtgcg ccggtgtgct cgcagaggtg
     1981 ctcgtgcccg aggacacgcc tatcagtccc ggccaagtgt tgggcaccat cgaagccgcg
     2041 ggaggcgaac cggtgtgagc tggagcctgg tggcacgcga cacgattgat gccggcgacg
     2101 atgtcatcgt cttggccgat gaccctgcgc ccatcgcatt ggccgcggcc gaggccgccg
     2161 gggcccgggg ttcggtccgc gtcgccgcat gcacggcgga ggccgagcgc catctctcgg
     2221 ccgtcctggc cggcaaggcc acggtgttcc gggcggctcc gggtgatctc tccaccgatg
     2281 tagtccgggc cgaggctcaa ctggcggcga cgccacccag aagcctggcc gaagcgcgtg
     2341 acgtcatcga ggaagctcga gcgcacgccc aagcgaaccc gatcatcccc gccgaatcgg
     2401 ccgatacggt tatcgcgata ggtgtgctcg ggcgtgtccc gccggcagag cgccgacacg
     2461 ccatcgagga gctctaccgc gtcctgcgcc ggggcggcac gtgcgtgctg tatgatgcgg
     2521 tgtccgacga accgttgccc gctcgcctgg ccgctgatcc cgcgatgccg gccgtgatgc
     2581 aggagctcga gctcctatca gccatggagg atgccaagtt ccacggactg accatcgagc
     2641 actggcccga tgagcccgac gccgtcttcg agggcatcga atgccgccgg atcgcgatca
     2701 tggcccacaa gggcaaggag ggtatttgcc gcgatggtct gcaggccgtg gtctatctcg
     2761 gaccgttcaa ggtcgtggaa gacgacgatg gccacgtaat ccgccgtggc cagcgcttcg
     2821 cggtgtgcaa gaagaccttc ggcgtctacc agcggccgcc gtacctgggg atgttcgcca
     2881 acctggagcc tccggaggag gtccccgatg agcaggcccc gctgtgggat ccggcgcatc
     2941 cgtccacgcg aagcccctcc gagaccaagg gcctcacgag gtcggctgcg gcagtgccgc
     3001 cgccagcggc cggcgctacg ggagaagcct atgccgctcg agccgtctgg ctgacccccg
     3061 acggccgggt ccgccggcaa gcccgcgtga cccatcgcta ccgcggctac ttcgagagct
     3121 acgaggaggc agagagcaca gctcgcaccg tgttcgagga cgccagccgg tacgaggtct
     3181 accggatctc gatgcaggag gccctggagc acatggccaa aacgggccgg caggcggcgt
     3241 gcccggtcac caaggggcca ggcggtggcc gagacggtgc tcgccgctag tgccttgagc
     3301 tcccccccac tccgtgccgc acaacgcggg ccggacggga agggctcgac gggcaccccg
     3361 cggaacctgt gtacccggca ctgccccgcg gcaagcccgg ctgtcgggct gccgcgatcc
     3421 attgactacc cgcgaccaga ggaggccagc atggccagaa tccccatcgc tctccagctc
     3481 tactcgatcc gcgatgactg cgcgaaggac ttccccggct gtttggaggc cgtagccaag
     3541 atgggctacg atggcgtcga gttcgctggc tatcacggac tctcggccaa ggagctcaag
     3601 aagatctgcg gtgacctggg tctggtgccc gtgggtgccc acgtgggcat ggacaccctt
     3661 atgggcgacc aactgcaggc gaccatcgac ttccacctcg agctcggcag caagtacccg
     3721 gtggttccgt ggatccccga ggagtaccgc cccgatgccg acggctggcg cagaacggcg
     3781 gacatgttca acgagatcgc ggcgcacctg aagccccacg ggctcaagac cgggtaccac
     3841 aaccaccaca tcgagtggac acctgtgggc ggggagatcc cgtgggacgt gttcttcagc
     3901 aacacctgtg ccgacgtcat catgcagctc gacctcggca atgcccttca tggcggcacg
     3961 cacgccgagc cgtacatcgg gaaatacccg ggccgttcca ccacgctcca tctcaaggag
     4021 ttctcggcca ccaacgacca ggcgctcatc ggcgagggcg atgtgcgatg ggaagaggca
     4081 ttcagactgg ccgagaccgt cgggggcacc gagtggtaca ttgtggaaca ggagagctat
     4141 gctttcccgc cgctcgagtg cgtggccagg tgcctcgaga acctgaggaa aatgggcaag
     4201 tagcgggccg gaccccggcc cggcgcctgt agggggagtg acaaccatgg gcgatcacga
     4261 tcagccacaa agcacgcgcc gggcgttcct gcgcgatgcg ctggtggcgg gaaccagcat
     4321 tgggctgctg ggagctgcga agcaggtcct cgccgccggc ccatgcgcaa ctcggtgccg
     4381 ccgacgcatt cccatcgccg tgcagctcta ctcggtccgc gacgactgcg ccaaggatct
     4441 gcccggcgtc ctcaaggccg tcagccagat gggctacgag ggcgttgagt tcgcggggta
     4501 ctacggccga tcggcccaag agctccgcac catgctcgac gacaacggcc tccgggtcgc
     4561 gggttcgcat gtcggcctcg agacgctgct cggcgacgag ctggagaagt cgatcgagtt
     4621 ccacaagacc atcgggaaca agtttctcat cgtaccggga ctgggcggcc agtacacggc
     4681 cgacaaagcc gcgtgggaga ggaccgcagg gatcttcaac gagatcgcgg ccaagctgca
     4741 gcccctgggg atgtacaccg gctaccacaa tcacaccatg gagttccaga agttccccga
     4801 tggcacgttg ccgtgggatg cgttcttcgg gaacacgcgt cagcgggtcg tgatgcagtt
     4861 cgatacgggc aacgccatga tcggtggcgc cagcgctctg cctttcctcg agaagtaccc
     4921 ggggcgggca ctcacggtgc atatgaagga gttttcgccc accaatggtg aggcgctcgt
     4981 gggcgagggc aacatcccct ggcgcgacat catccgcgtc tgccgcacca ccggcggcac
     5041 ggagtggttc atcgtcgagc aggagagcta tgcctatccg cccatcgagt gcatccggcg
     5101 atgtctggtg aacctgcgca tgctcgtgag cctgtagtcg gccggtagtc gtagacacag
     5161 cacagggccg gaggcgtgcg tcccctccgg ccctgttcga tcagagcgag tgctccgcca
     5221 ggggtccggt cgctctctat ccaccagggc acccgtcacc tctcagcgtc ccctgacccc
     5281 gttcaccatt accaacgcga ggcgcctggc gcgagttccc tgccctactg agtggggatg
     5341 acctggtcca gcgccgcttc gtctcccacg ctgcggacgg ttcctccgcg gggtgcgggc
     5401 actcccccgc cgttacgcgc cggggccgga gtgggcctcc gcgacgactc ggccgctggt
     5461 ttcacgctcg gggcggcact cgagccgctg cccaacgatg tcgccgatcg cgcgccaccg
     5521 gctttcggga ggggcgccgg ggccggagcg gctttgggcg aagccgacgg ccggggcgcc
     5581 tcggccccac gcacggacgc agcagccggc gcggccttgg agggcccggc caacggagcc
     5641 agcgcgcgcg actcgccagc cggccccccg tccttctgtg ttggcgggac ctcggtagct
     5701 cccgcaagcg ctcctccggc cgcctgcggt tcggacgggc ttgctggtgg ctcatcgagc
     5761 gcctgcttgc gttcttcggg tggcggagcg ggagacggca ggggcgcctt cagcagcgca
     5821 ccgacgaaca ggagccccat gatcagcgcc agggccacgt gcgggccggc gccggcccgg
     5881 ctcggaccat cgttccgctg gcgagccacg tagacgaccg ttgccgctgt gagtccagtg
     5941 gccaccagag cgagcaccac ctgcgcggcc cgcacgagga acggagtgaa caggtcgaag
     6001 ccacccggt
//
LOCUS       DAOSPL010000060         5992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_13379_length_5992_cov_2.971029, whole genome shotgun sequence.
ACCESSION   DAOSPL010000060 DAOSPL010000000
VERSION     DAOSPL010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13379_length_5992_cov_2.971029"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="PLQ54_01945"
     CDS             <1..821
                     /locus_tag="PLQ54_01945"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HOS92048.1"
                     /translation="SACRRGPMLVMLGAAAVAVVLVALFAVTARPHERAPRAGSRPSL
                     AVRPPAAPEPPTVSSSALEAEVSVTNDRVRMNELAQAVAAYRHDTGKLPATLGALAQP
                     DAGLAGYCGPYVRELPRAAGGRRFVYDDHTGAVSLVDADGTPVDSASPAAGDASPSAS
                     SGARAVANAHLAALSAAVSAYQRDHGANPPSLDALCESAGGKGPYLRRLPHPPGGGDY
                     RYTMETATVSWGNLSRTNATLLLDPEKAQFPSRDALSGKGPLGVNLPPLPTRAR"
     gene            904..2586
                     /locus_tag="PLQ54_01950"
     CDS             904..2586
                     /locus_tag="PLQ54_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOS92049.1"
                     /translation="MSEGESSGASVWHQGRLLLGVSPGEPFNACERVVDRNCARDPEA
                     TAAIHRNHHGDRRVFTFEDLRRAVSRFASALDALGVNPGDRVFTLLEPMPELFWSILG
                     AIRTGAIAGPLFAAFGSEAIRDRLGDSGARVLITHGRHLLKVRAAIEGLPGLKQVLVV
                     DPPADGLRGIERDLAALLADASDERPPHPTSEQDPMLLHYSSGTTGKPKGVLHVHEAL
                     VGHAATARAVLDLRREDSYWCTADPGWVTGTSYALFGPWALGVPQIHYEGGFSSSAWY
                     SLIEAERVSVWYTSPTALRMLMRDGVEAARAHDLSSLRHICSVGEPLNPEVIRWGLET
                     YGHTIRDTWWQTETGCMQIANAPWLPVRPGSMGKPLPGVTAAVLDPTTHEPLPPGQEG
                     LLALVPPWPSMFRTYWNRLDLYGARFRNGFYLTGDRAYTDADGYYWFVGRDDDVINTS
                     GHLVGPFEIESALLTHPAVVEAAAFPIPVADAGEAVAARVVLAAGSESSRELIRDLKT
                     LVRRQVGPYAVPREVVVVDRLPRTRSGKIMRRVARAEHLGLPVGDLSSLDES"
     gene            2611..2862
                     /locus_tag="PLQ54_01955"
     CDS             2611..2862
                     /locus_tag="PLQ54_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOS92050.1"
                     /translation="MDEWKQKLLEYIRDHLAQDPSQVTSTDQPLISTGIMDSFALVEL
                     SVFIEEEFGVSVPDPEMTAEHFDTIDLGVATIARFADDA"
     gene            2959..3243
                     /locus_tag="PLQ54_01960"
     CDS             2959..3243
                     /locus_tag="PLQ54_01960"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HOS92051.1"
                     /translation="MIGVDSHLRPKPDVIVTDLDDEAVLLDMEAKRYFGLNDTGLVVW
                     RTVAQGGTPGDAASALVREFEVTEAEALESTLDLCGDLVEAGLLVPVEAR"
     gene            3250..4197
                     /locus_tag="PLQ54_01965"
     CDS             3250..4197
                     /locus_tag="PLQ54_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92052.1"
                     /translation="MGLASLCTDFHGVTLEVSADRADVLVAVRPGLSRLPGGSGATGV
                     RIALRNCRQVPTPHTSSRLFRHGGVQGLQDGAALWLVADGCAVRIDEHATEASGWVTP
                     AARGDGVSEVVHLALLELLRRRGLYHVHAACVVSEAGAVLLPADGRAGKTTLTLAALR
                     AGWRLVGDDAVWLRDGPDGIQALAWPEPLHVERETAHRLGLTDSLGAPASAGRWELDP
                     ERAFPGSCVASARPIAVVFPVIAATETSGLTSLGRAEALARLLRQSPLLFTHLERAGG
                     HLAVLRRLVTQCRCAELRSGAEVLSGVLPEALRAGLGLS"
     gene            4194..4610
                     /locus_tag="PLQ54_01970"
     CDS             4194..4610
                     /locus_tag="PLQ54_01970"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HOS92053.1"
                     /translation="MTLWGRFGSRLEAAALLLVAGPAYRLLPTNTFLRWWTPPPRRRC
                     GMRVVDVDGLARAVDDVLAVSRRRNCLTRSLMLYRMLRRRGVEVELRIGVRREGAEGI
                     AGHAWLLLDGSPCCPYGPDPSGYVVTLDYPNEGPDH"
     gene            complement(4702..5703)
                     /locus_tag="PLQ54_01975"
     CDS             complement(4702..5703)
                     /locus_tag="PLQ54_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOS92054.1"
                     /translation="MRKLRAVVVLSILLGAVAAQASITESFTASGGWLIDWGLSVVLD
                     SGWAAGSSSGRHLSFNHGGTSLAGEGFYLYDGLEVYYNVSDYNKLPSASDNEPSGGEL
                     YDIEALLAAYDAATNDWYVMGITSHGSRENNTVIGDLAINVEDSWEWGSTYGTITSGL
                     NTSELGIKLGGKQAGHVEANPDWGYGWDGRDLYGADRRNPWTDNGTNYGGTDPTKSSK
                     RTQGYKPGVNTDQVWGSNYVYGSGDSSWELAGDQFTGLNPIFGTWGDGSGSSSKTYAF
                     AVNIDGNWLSNGIQNVSWTESCLNDDGRLKTPEPATAALLAVAGAGLVARRVRRRSQ"
BASE COUNT          851 a         2012 c         2080 g         1049 t
ORIGIN      
        1 tcagcgcctg ccggcggggg ccgatgctcg tcatgctggg cgccgccgcg gtcgctgtcg
       61 tgctcgtcgc gctgttcgcc gtgacggcca gaccgcatga gcgtgcgccg cgggcgggct
      121 cacggccgag tttggcggtg cgtccccccg cggcccccga gccgccgacg gtttcgagca
      181 gcgccctcga ggccgaggtg agcgtcacca acgaccgcgt tcgcatgaac gaactggccc
      241 aagcggtagc ggcctaccgg catgacacgg ggaagttgcc agcaacactc ggcgcgctgg
      301 cgcagccgga tgctgggttg gcggggtact gcgggcccta cgtgagggag ctccccaggg
      361 cagctggggg ccgccgcttt gtgtatgacg accacacggg agcggtctcg ctcgtcgatg
      421 ccgatgggac ccctgttgac tcggcatcgc cggcggcggg agacgcgtcc ccatcggcct
      481 cgtccggcgc acgggcggtg gcgaatgccc acctggccgc gctcagcgcc gccgtgtcgg
      541 cctaccagcg tgatcacggc gcgaaccccc cttcgctcga tgcattgtgt gagagcgccg
      601 gtggcaaggg cccatacctg cggagattgc cccaccctcc ggggggcggc gactatcggt
      661 acacgatgga gaccgcgacg gtctcgtggg gcaatctctc gcgcaccaac gccacgttgc
      721 tgctggaccc cgagaaggcc cagttcccga gccgcgacgc gctctcaggc aaggggccgc
      781 tgggcgtgaa cctgccgccg ctaccgacgc gtgccaggtg atccttgcgg acgtatgcct
      841 gccctgggta tcgtgtgcct gatcctcggg gagcgccgct gatgtgcgag gggcatcgga
      901 tccatgtcag agggagagtc atctggcgcc tcggtgtggc accaaggccg gctactgctc
      961 ggagtctccc ccggggagcc gttcaacgcg tgcgagcgcg tggtcgaccg caactgcgcg
     1021 cgtgacccgg aggctaccgc cgccatccac cgcaaccatc acggagatcg ccgggtcttc
     1081 acctttgagg atctgcgacg ggccgtctcc cgtttcgcgt ctgcccttga cgctttgggt
     1141 gtgaatccgg gcgaccgggt gttcactctg ttggagccca tgcccgagct gttctggtcc
     1201 attctggggg ctatccgcac gggcgccatc gcgggcccat tgttcgcggc gtttggctcc
     1261 gaggccatcc gcgatcgcct cggggacagt ggggcccgcg tgctgatcac gcacgggcgg
     1321 catctgctca aggtgcgtgc ggccattgag ggtctgccgg gtctgaaaca ggtgctcgtc
     1381 gtcgacccgc cggccgacgg gttgcggggg atcgagcgcg acctggccgc gctgctggcc
     1441 gatgccagtg acgagcgtcc gccacacccc acgtcggagc aagaccccat gctgctccac
     1501 tacagcagtg gaacgacggg gaaaccgaag ggtgtgcttc acgtccacga agcgctggtc
     1561 ggtcacgcgg ccacggcacg ggccgttctc gacctgagac gcgaggactc ctactggtgt
     1621 acggccgacc ccgggtgggt cacgggcacc tcctatgcct tgttcggccc ctgggcgctg
     1681 ggggtgccgc agattcacta cgaaggcggc tttagctcca gcgcctggta ctcgctgatc
     1741 gaggcggaac gtgtcagcgt ctggtacacg tcaccaacgg cgctgcgaat gctgatgcgc
     1801 gacggggtcg aggcggctcg cgcgcacgat ctgtcctctc ttcgtcatat ctgcagtgtg
     1861 ggtgagccac tcaatcctga ggtgatccgg tggggcctcg aaacctacgg gcacaccatc
     1921 cgtgacacat ggtggcagac cgagaccggg tgcatgcaga tcgccaacgc gccctggctc
     1981 ccggtgcgac cggggagcat ggggaaacca ctcccgggtg tcacggcggc tgtgctcgat
     2041 ccgacgacgc acgagcccct gccgccgggc caagaggggt tgctggcact cgtgccgccg
     2101 tggccctcga tgttccgcac ctactggaac aggctggacc tgtacggtgc ccggttccgc
     2161 aatggcttct acctgacggg ggatcgtgcc tacaccgacg cggacggcta ctactggttc
     2221 gtgggccgcg atgatgacgt gatcaacacg tcgggccact tggtgggccc gttcgagatc
     2281 gaaagcgccc tgctcaccca ccccgctgtc gtcgaggcgg cagcgttccc gatacccgtt
     2341 gccgatgccg gcgaggccgt ggcggcccgg gtggttctgg ccgcggggtc ggagtcctcg
     2401 cgcgagctga ttcgagatct gaagacactg gtgcgccgcc aggtggggcc ctacgcggtg
     2461 ccccgcgagg tggtcgtcgt ggacaggctt ccgcggaccc gaagcggcaa gatcatgcga
     2521 cgcgtcgccc gagcggagca cttgggtctg ccggtggggg acctgtcgtc cctcgatgag
     2581 tcctagcctg cactgaggag tcgattccac ttggacgaat ggaaacagaa gctgctggag
     2641 tacatccgcg atcatctggc tcaggaccca tcgcaggtca cgtcgacgga ccagcccctc
     2701 atctcgacag ggatcatgga cagctttgcc ctggtcgagt tgtcggtgtt catcgaggag
     2761 gagtttggcg tctcggtgcc cgaccctgag atgacggcgg agcacttcga caccatcgac
     2821 ctgggcgtcg ccaccatcgc tcggttcgcc gacgacgcgt gacgcggggc agcggaggcg
     2881 cgagtgcggg gcgtttggtc atgaagtccg cgggacgcgg tacaatgggc gtcagctgtt
     2941 cccgtggacg gtggtgccat gatcggcgtc gacagccatc tccgcccaaa gcctgacgtg
     3001 atcgtcaccg acctggacga tgaggccgtg ctgctcgata tggaggcgaa gcgctacttc
     3061 gggctcaacg acaccggtct cgtcgtgtgg cgcacggtcg cccagggggg tacccccggc
     3121 gatgccgctt cggcgctggt gcgcgagttc gaggtcaccg aagcggaggc gctggagtcc
     3181 acccttgacc tgtgcggcga cctggtcgag gcgggtctgc tcgtccctgt cgaggcacgg
     3241 tgaccggcgg tgggtctggc gtcgctgtgc acggacttcc acggcgtgac gcttgaggta
     3301 tccgctgatc gcgccgacgt tctggtcgcc gtccgccccg ggctgtcgcg actgccgggg
     3361 ggctcgggtg ccaccggtgt ccgcatcgcg ctgcgtaact gccgccaggt gcccacccca
     3421 cacacctcat cacgcctgtt ccgccacggc ggcgtgcagg ggctccagga tggcgcggcc
     3481 ctctggctcg tcgccgacgg ctgcgccgtg cgaatcgacg agcacgccac ggaggcctcg
     3541 ggctgggtta cccccgccgc gcgcggcgac ggcgtttctg aggtggttca cctcgccctg
     3601 ctggagctgc tccgccggcg agggctctat cacgtgcatg ccgcgtgtgt cgtgtcggag
     3661 gcgggcgccg tgctactgcc agccgacggg cgggcgggga agacgacgct gacactggcc
     3721 gcgcttcggg caggctggcg cctggtcggc gacgacgccg tgtggctgcg cgacggaccg
     3781 gacggcatcc aggcgctcgc gtggcccgag ccgctccatg tcgagcgtga gacggcgcac
     3841 cgcctcggcc tgacggactc cctgggcgct cccgcgtccg cggggagatg ggagctcgat
     3901 ccggagaggg cattccctgg ctcgtgtgtg gcctcggccc gccccatcgc ggtcgtgttc
     3961 cccgtcatcg ccgcgactga gacgtcgggg ctgacgtcgc tgggccgcgc tgaggccctg
     4021 gctcgcctgc tgcgccagag ccctctgttg ttcacacacc tcgagcgcgc tggcgggcac
     4081 ctggccgtcc tgcggaggct cgtcactcag tgccgttgtg ccgagctccg gtccggcgca
     4141 gaggtcctct ccggcgttct tcccgaggcg ctccgggccg ggttgggtct gtcgtgactc
     4201 tctggggccg cttcgggagc aggctcgagg cggccgcatt actgcttgtg gccggccccg
     4261 cgtaccgcct actgccgacg aacacctttc tccgatggtg gactcccccg ccacggcggc
     4321 ggtgtggcat gcgggtggtc gacgttgacg gcctcgcccg cgccgtcgac gacgtcctcg
     4381 ccgtgagccg ccgccggaac tgcctgactc gatccctgat gctctaccgc atgttgcggc
     4441 gtcgtggggt ggaggtagaa ctgcggatcg gtgttcgccg cgagggcgcg gaggggatcg
     4501 ccggacatgc gtggttgctc ctcgatggca gcccctgttg cccctatgga ccagatccgt
     4561 cgggctatgt cgtgacgctg gactacccga acgagggccc tgaccactga aaggcccatg
     4621 gcggagagac tggcggggac ccgctgccag tggcaacgag cccccacgcc gatccatccc
     4681 ccgtcttggg tctgcgggcg cctattggct gcgccgacgg acgcgccggg ccacgaggcc
     4741 cgcgccggca accgcaagca gtgccgcggt ggccggctcc ggcgtcttca gccggccgtc
     4801 gtcgttgagg cagctttcgg tccaggagac gttctgaatg ccgttcgaga gccagttgcc
     4861 gtcgatgttc acggcaaagg cgtaggtctt gctcgacgaa ccgctgccgt cgccccatgt
     4921 accgaagatc gggttgaggc ccgtgaactg gtcgccggcg agctcccagg aggagtcgcc
     4981 ggatccgtag acatagttgc tgccccacac ctgatccgtg ttcacgccgg gcttgtagcc
     5041 ctgggtccgc ttcgagctct ttgtcggatc cgttccgccg tagttggtgc cgttgtcggt
     5101 ccacgggttg cgcctgtcgg ccccgtacag gtcacggcca tcccagccat agccccagtc
     5161 ggggttggcc tcaacgtgac ccgcctgttt gccgcccagc ttgatcccca gctccgacgt
     5221 gttcagtccg gacgttatgg tgccataggt tgaaccccac tcccacgaat cctcgacgtt
     5281 gatggccaag tcgccgatga ctgtgttgtt ctcgcggctg ccatggctgg tgattcccat
     5341 tacataccag tcgttggttg cggcgtcgta ggcggccagg agggcctcga tgtcatagag
     5401 ctccccgccg cttggttcgt tgtcggacgc gctgggcagc ttgttgtagt ccgagacgtt
     5461 gtagtagacc tccagcccgt cgtagaggta gaaaccctct ccggccaggc tggtgccccc
     5521 atggttgaag gagagatgcc ggcctgacga cgagccggcc gcccagccgc tgtcgagaac
     5581 cacgctcaga ccccagtcaa tgagccaccc accagacgcg gtgaagcttt ctgtgatact
     5641 cgcctgtgcc gccacggccc cgagcagaat gctcaggacg acaaccgcgc gcaacttgcg
     5701 cactctgcca cccctccagt tccgttcaac tccgcccgcc ctgcaacgca gggaggccaa
     5761 gcggtagcca gcggtggccc agcagaccag agcgggcggt acagcccgat gatgtccata
     5821 ctgagtcacc acaggagtac ttccacagct gggaggccat cgggcgcagg tggggcgtat
     5881 tcgtcgcgcc tttcgtctgc ggccaccgac cacgggcggc gggtcttggc ctgatgtctg
     5941 gcacgcaccc gcttggggtc cgcggccaag ggggcacacg acgctgaagc cg
//
LOCUS       DAOSPL010000061         5913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_13613_length_5913_cov_2.849949, whole genome shotgun sequence.
ACCESSION   DAOSPL010000061 DAOSPL010000000
VERSION     DAOSPL010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13613_length_5913_cov_2.849949"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..1141
                     /locus_tag="PLQ54_01980"
     CDS             332..1141
                     /locus_tag="PLQ54_01980"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HOS92055.1"
                     /translation="MAWSIAVSCWAMVIAAVWLGMWAVRRGPRCRCATGTAAAAVLAA
                     DLATVAWGGSRSVWWVPEWGWWQVHLPCALLTVLVITLLPRTPATWLRRPLTGLLIGF
                     VAYQCALAAGPPAVAANVFGLSTLSGERHLLQSTGWSCGAAATAIVRSGWGVPTSEYE
                     AAVACGTRPWLGTSTLGMCEGLLAAGLRPEERCLAKWSDLVAAPKPCLACTVIFGGVL
                     HVVAVLRADANSVTVHDPIVGVSDLSRAEFEEQWIGAIVFVRGPMRSPARR"
     gene            1188..4478
                     /locus_tag="PLQ54_01985"
     CDS             1188..4478
                     /locus_tag="PLQ54_01985"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOS92056.1"
                     /translation="MLTTYALIGALAAGGNPAATIDATVPDDWQTPLVLTVDVDGLRV
                     LAGADDGTIPRVYDAERGPCATQVLAGLAGPQLAWLPPVSGERRFEIRFEAGAEEARF
                     GQLTVEETDGEIRVSTGAFTAVHSLTANAGALARIEYADGGEPDVLQMCGDRIGIEGM
                     YFARDDRSPRVEIIDQRPLACTVRAHAMFCDAAGAPAPGRPEVTYLYTYFAGSPLVRI
                     EALVTQPEPRSWGELNLFQRVYKKLRYPSWIVSSPDRTGTLTGANASYVSTTLAGMFD
                     ARHAIGLMGMPCRIYDEDLALAGGWGSYIHGPWVTMSDTRYHCSGWLYVGPSGGDGSA
                     LTAQARALEALRLSARVRPLEFEVERIRDRLPRLRGRARLLTEAALGLTSSVVGDLSD
                     LERSQEAVKHLAALVSGEGPEAGSQQVVGNDLMSVLLGPAEDGFPVRGLRAANGDRDF
                     GPGAAGGGLWRLRFRDEAGTIAEVTSASPSEHEADGTEDGRKLKLRWRGIAVGDEPRA
                     ADVEVSARIGRGARQSLWSIRVKTLSTKWALWEIDFPYLSGLRVDPDAGTVAYPRFLG
                     YLERKPLINESPDLIYPSHAAEMQMSSVQSGNSALYFATYDGAAWHKRFKFRPEEGRS
                     LSYIATHYPPYTPGKPGTFELPYETAIGVFEGDWIDSCELYRQWALQQQWCQLGPLAE
                     RKDIPKWFVDLGAWFVSAPSDQAIERSKAVGVPMAIQRYGWHQIPFDDDYPDYFPAKD
                     EFPGEIQRMHAGGIRVVPYINGRLWDTDTESFANEGAQQSCTKDVNQERYIENWGQQN
                     HSVQCPSTANWQRKVAGIVERMARELDVDGVYIDQIASYYPMRCFDPTHPHAAGTGEY
                     WVPSYRKLVELCQQRGRAAKPDLALTTEDHAEPFLDLFDGCLTCNAAFAASGLIPMFH
                     HVYSGYGILYGRYNTPPDSDEHALTFRQKNAQMLVWGTQIGWLDDTQLGHREEETAYF
                     AALAKAYLAARPFCLYGRMLRPMVMAGDAPELSAEWHPGQVATMPAVLHSAWESREGE
                     LGLIATNTDTVPHTVSLRVPRGWTTDRVTGLEEVYRSGDCIYRVEGGRRVRLTLPARG
                     IVVLRLEA"
     gene            4574..5809
                     /locus_tag="PLQ54_01990"
     CDS             4574..5809
                     /locus_tag="PLQ54_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92057.1"
                     /translation="MLNQRDIAIIRGLAARVAEIAAMPVQEEKRRLWRKLNALNPERP
                     MVMIDQVCWNEMNYGGALNIECEDPECQAYEGHLRRLLLQWDHFPVDMVVEPFILVPK
                     AIHNTGFGVGVREQIAVTDPTNAVVGHKFENQFQTEDDLEKIQTPRISHDTDETARRM
                     AVAHELFDGLLEVRASGCDPYVSLWDPISTWMGVENALYTLIDRPDYMHRLVGRLTDA
                     YMAMLDQLESQGLLCQPQCLIHCTGAYTDELPAPGYDPERPRTQDLWVFGLAQMFSTV
                     SPTTFKEFEVDYASRICGRFGLVYYGCCDPLDGKMAEVRLIPKVRKVSMSPWVDEQRG
                     AREIGSDYVYSRKPNPAVFAPDHFHPDEVRESLTTTRNICEQHGCPLELILKDISTVR
                     YDPERLFEWSRIAMEVVGG"
BASE COUNT          968 a         1946 c         2043 g          956 t
ORIGIN      
        1 gagcgcagcc agggcgagca agagcgcccg caccggagtc gagggatggt tcatggcagg
       61 cccttcctgt ggtgctcgga gcactcgcac cggcgggggc taccgagccc acgaccccaa
      121 tctccgtcta agcccactgg cctgatcgga ggtccgtctt tcggcagact ccgcacgggc
      181 gccgatcgct cccacagagg ggtgttcgcg cctggccgaa gccgggcctc cgcccaagcc
      241 cggtcgtgga gcgcccgagt ggcccaaccc ccgaccttct tcgcccatcc aagtggtcga
      301 gacgcccatc gatctctgga ggtgactcgc catggcgtgg tcgatcgcgg tgtcgtgctg
      361 ggccatggtc attgcggcgg tatggcttgg catgtgggcc gtccgccgtg ggcccagatg
      421 ccggtgtgcg acgggcacag cggcggccgc tgtgctggcc gccgatctgg caacggtcgc
      481 ctggggcggg tctcgctccg tgtggtgggt gcccgagtgg ggctggtggc aggtccacct
      541 gccatgcgcg ctgctcaccg tgctcgtgat cacgctgctc ccccgtaccc ctgcgacgtg
      601 gctgcgccgc ccgctgaccg gcctgttgat cgggttcgta gcctaccagt gcgcgctggc
      661 cgccggacct ccggccgtgg cggcgaacgt ctttggactg agcactctct ccggggagag
      721 acacctgctg cagagcaccg gatggagctg tggtgcagcg gccacggcga tcgtacgctc
      781 cggctggggc gtgccgacga gcgagtatga ggcagccgtt gcctgcggca cgcggccgtg
      841 gttgggcacc tcgacgctgg gtatgtgcga ggggctgctg gcggccggac ttaggccgga
      901 ggagcggtgc ctcgccaagt ggagcgacct cgtggccgcg ccgaagccgt gcctggcgtg
      961 cacagtgatc ttcggcggcg tacttcacgt cgttgccgtc ctgcgggccg acgcgaactc
     1021 ggtgaccgtc cacgacccca tcgtgggcgt gtcggacctc tcacgagccg agttcgagga
     1081 acagtggatc ggcgccattg tgttcgtgcg gggcccaatg aggtccccgg cgcgccggta
     1141 gcgaagcatg tatcccgcgg gcggtccgtg cccccgaagg gactcacatg ctcactacat
     1201 atgcactcat cggcgcacta gcggcaggcg gcaatcccgc ggccaccatc gacgcgacgg
     1261 tcccagacga ctggcagacg ccgctggtgc tcacggtcga tgtcgacgga ctgcgtgtgc
     1321 tggccggcgc tgacgacggc acgattcccc gcgtctacga tgcggagcgg gggccgtgcg
     1381 ccacgcaggt ccttgcgggt ctggccggtc cgcaactggc gtggctccct ccggtcagcg
     1441 gggagcggcg gtttgagatt cgctttgagg ccggcgcgga ggaagcgagg ttcggacagc
     1501 tcaccgtcga ggagaccgac ggggagatcc gcgtctcgac cggcgccttc acggccgtgc
     1561 actcgctgac cgccaatgcg ggggcgctgg cgcggatcga gtacgccgat ggcggcgagc
     1621 cggatgtgct ccagatgtgc ggcgaccgca tcggcatcga agggatgtac ttcgcccgcg
     1681 acgaccgctc gccccgcgtc gagatcatcg accagcggcc gctggcgtgc accgtgcggg
     1741 cccacgccat gttctgcgat gccgcaggcg ctccggcacc ggggaggccc gaggtcacct
     1801 acctctacac ctacttcgcc ggcagtccgc tggtgcgcat cgaggcgctc gtcacgcagc
     1861 cagagcctcg ctcctggggg gagctgaacc tgttccagcg ggtttacaag aagctccgct
     1921 acccctcgtg gatcgtctcg tcgcccgacc ggacgggcac gctaacgggc gccaacgcgt
     1981 cgtacgtcag caccacgctg gcggggatgt tcgacgcgcg gcatgccatc ggcctcatgg
     2041 gcatgccatg ccggatctac gacgaggacc tggcgctggc cggaggctgg gggagctaca
     2101 tccacgggcc ctgggtgacc atgagcgaca cacgctacca ctgcagcggg tggctctacg
     2161 tcggcccgtc gggcggtgac ggctcggcgt tgacggctca ggctcgggcg ctcgaggcac
     2221 tccgcctgag cgcgagagtt cggccgctgg agttcgaggt ggagcgaatc cgcgaccgcc
     2281 tcccgcgcct cagaggccgc gcgcgcctgc tgacggaagc ggcacttggg ctgaccagct
     2341 cggtcgtcgg ggacctgtcc gacctcgagc gttcacagga agcggtgaag cacttggcgg
     2401 cactcgtgag tggggaggga cctgaggcgg gctcgcagca ggttgtgggc aacgacttga
     2461 tgtctgttct cctcggcccg gccgaggacg gtttcccggt ccgcggcctc agggcggcga
     2521 acggcgacag ggacttcgga cccggcgccg cgggcggcgg gctctggcgg cttcggttcc
     2581 gcgacgaggc cgggaccatc gccgaggtca cctcggccag tccgtcggag cacgaggccg
     2641 acgggaccga ggatgggcgc aagctcaagc tccgctggcg cggtatcgcc gtgggcgacg
     2701 aaccccgcgc cgccgacgtg gaggtcagcg ctcggatcgg gcgcggcgcc aggcagtcgc
     2761 tgtggagcat ccgtgtcaag acgctcagca cgaagtgggc gctgtgggag atcgacttcc
     2821 cctacctgag cgggctgcgg gtggatccgg atgccggcac ggtggcgtat ccgcggttcc
     2881 ttgggtacct cgagcggaag ccgctcatca acgagtcgcc ggacctgatc tacccctcgc
     2941 acgcggcgga gatgcagatg tcgtcggtgc agtcgggcaa ctcggcgctc tacttcgcga
     3001 cctacgatgg agccgcgtgg cacaagcggt tcaagttccg accggaggag gggcggtcgc
     3061 tctcatacat cgcgacacac tacccgccct acacgcccgg caagccgggc accttcgagc
     3121 tgccctatga aacggccatc ggtgtgttcg agggcgactg gatcgactcg tgcgagctct
     3181 accgccagtg ggcgctgcag cagcagtggt gccagcttgg gccgttggcc gagcggaaag
     3241 acatcccaaa gtggttcgtg gaccttgggg cgtggttcgt ctcggcgccg agcgaccagg
     3301 cgatcgaacg gagcaaggct gtcggcgtgc cgatggccat tcagcgctac ggttggcacc
     3361 agatcccctt cgatgacgac tatcccgact acttcccagc taaggacgag ttccccggcg
     3421 agatccagcg aatgcacgcc ggcggcatac gcgtggtgcc ctacatcaac ggccggctgt
     3481 gggacacgga caccgagagc ttcgccaacg agggcgcgca gcagtcatgc accaaggacg
     3541 tgaaccagga acggtacatc gagaactggg gccagcagaa ccactcggtg cagtgcccat
     3601 ccaccgccaa ctggcagagg aaggtagctg gcatcgtcga gcgcatggcg cgcgagctcg
     3661 acgtcgatgg cgtctacatc gaccagatcg cgtcgtacta ccccatgcgg tgcttcgatc
     3721 cgacccaccc gcacgcggca ggcaccggcg agtactgggt gccgagctac cggaagctcg
     3781 tcgagctttg ccagcagcgc ggccgggcgg caaagcccga cttggcgctc acgaccgagg
     3841 accacgcgga gccattcctg gacctgttcg acggctgcct gacgtgcaat gccgcgttcg
     3901 cggcctcggg cttgatcccc atgttccacc atgtctactc gggctacggc atcctctacg
     3961 gccgctacaa cacgccgccg gactcggacg aacatgccct gaccttccgg cagaagaacg
     4021 cgcagatgct ggtctggggc acccagatcg gctggctcga tgacacccag ctcggccatc
     4081 gggaggagga gacggcgtac ttcgcggcac tggcgaaggc ctaccttgcc gccaggccct
     4141 tctgcctcta cggccggatg ctccggccga tggtgatggc gggcgatgcg cccgagctct
     4201 cggccgagtg gcatcccgga caggtcgcga ccatgccggc ggtgctgcac tcggcgtggg
     4261 agtcccgcga gggcgagctg gggctgatcg cgacgaacac cgatactgtg ccgcacaccg
     4321 tatcgcttcg cgtgccccgc gggtggacca cagaccgagt caccgggctc gaggaagtct
     4381 accgctcggg tgactgcatc taccgcgtgg agggaggccg ccgtgtgcga ctgacgttgc
     4441 ccgcgcgggg tattgtggtg ctcagactgg aggcatagct gcaaggtcca gcagtcgggg
     4501 ccggtcagcc atggggcgcg ccccttcgaa cagcgccgtg ggcgctcgcg agttgcacag
     4561 aggagacgtc gacatgctca accagcgcga tatcgccatc atacggggcc tggcggcacg
     4621 cgtcgccgag atcgctgcca tgccggtgca agaggagaag cggcgtctct ggcgcaagct
     4681 caacgcgctc aaccccgagc ggcccatggt catgatcgac caggtctgct ggaacgagat
     4741 gaactacggc ggcgcactca acatcgagtg cgaggacccg gagtgccagg catacgaagg
     4801 ccatctccgg cgcctgctgc tccagtggga ccacttcccc gtcgatatgg tggtcgagcc
     4861 attcatactg gtcccgaagg caattcacaa tacgggcttc ggcgtaggcg tgcgtgagca
     4921 aatcgccgtc accgacccga ccaatgcagt ggtcggccac aagttcgaga accagttcca
     4981 gactgaggac gacctcgaga agatccagac accgcggatc agccacgata ccgacgagac
     5041 ggcgcgccgg atggccgtgg ctcacgagct gttcgacggc ctcctcgagg tccgagcctc
     5101 cgggtgtgat ccctatgttt cgctctggga ccccatctcg acgtggatgg gcgtcgagaa
     5161 cgcgctgtac accctgatcg accggccgga ctacatgcat cggctagtgg ggcgcctgac
     5221 cgatgcatac atggccatgc tcgaccagct cgagagccag gggctcctat gccaaccgca
     5281 gtgcctgatc cactgcaccg gcgcgtatac cgacgagctg cccgcccccg ggtacgatcc
     5341 ggagaggccc cgcacccagg acctatgggt gttcgggctc gcgcagatgt tctcgacggt
     5401 ctctccgacc acattcaagg agttcgaggt cgattacgcc agtcgcattt gcgggcggtt
     5461 cgggcttgtc tactacggct gctgcgaccc cctcgatggc aagatggccg aagtgcggtt
     5521 aatcccgaag gttcgtaaag tgtcgatgag cccttgggtc gatgagcagc ggggggcgag
     5581 ggagatcggc tccgactatg tctactcgcg caaacctaac cccgccgtgt tcgccccaga
     5641 ccacttccac ccggacgagg taagggagag cctgacgacc acacgcaaca tctgtgagca
     5701 acacggctgt ccgctcgagc tgatcctcaa ggacatcagc accgttcgct acgatcccga
     5761 gcgtctcttc gagtggtcgc gcatcgccat ggaggtcgtt ggcggctgat gcgggctcca
     5821 cggaaggcgg taaccacgat gatgcggatc acacccgcgg ttgcgctggt ggcattgttg
     5881 ggcacctcgg ctctggcctg gggccaaacc agg
//
LOCUS       DAOSPL010000062         5888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_13696_length_5888_cov_3.340477, whole genome shotgun sequence.
ACCESSION   DAOSPL010000062 DAOSPL010000000
VERSION     DAOSPL010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13696_length_5888_cov_3.340477"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PLQ54_01995"
     CDS             complement(<1..670)
                     /locus_tag="PLQ54_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92058.1"
                     /translation="MRMSRVLIVGGGPSGCACALALRCGAQARGESVEVILLEPKRFG
                     RHFNQCAGVVMPSQIDEVLGEWGTSLPRRLIQREVGGYVLHAEQDRIELPPRPGDPAS
                     SATRRVRLDQHLLNEAHRAGTLIVPDRASDIEIGDDGVVVYTDGDTYHADAVIGAFGL
                     DPGTAGVFARCTRYRPPPALSTLVTKVHPHGLEPVPGLLGDQIHAFLPRLPGVEFGAL
                     IPKGN"
     gene            complement(667..2022)
                     /locus_tag="PLQ54_02000"
     CDS             complement(667..2022)
                     /locus_tag="PLQ54_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOS92059.1"
                     /translation="MDTLREPRSIAIIGGGPAGALTALHLLRLAREKGRSIDVTVFDR
                     KEFGVTGPRGCNMCAGVVSNSLIVRLERLGVSIPSSAVQRAVDGYCIHSGGQVVRLEK
                     PVGSRIYTAFRSGGPMGEEFGTARSFDHALLAAAVRGGARHIRRVVIGIERPAGARDP
                     VVITDARGGRHLADAVVGAFGVNSRTVLTFRALGFGYSPPRTAMAAQCEIPLPSAEID
                     RRFGSDIHVLAVGMEGVIVAVMVPKERHVTVSLIGPWVGQAHLERFMRQPEVGRLLPE
                     GFHMPERYCHCHPLLPITGARNVVADRIVVVGDAHISRYLKNGLDSAFTTAELAAHAI
                     IEGGVDRTSLVEWYVKPCRRAFVWDNRSGRILFSAYQALGRHPSLTRRICAAAQDESD
                     SAAPLRSIAWGLLTGDEPYAALLRQALSPRLHWHWAIGATRGLRTRDTGAADRPKEGP
                     S"
     gene            complement(2049..2996)
                     /locus_tag="PLQ54_02005"
     CDS             complement(2049..2996)
                     /locus_tag="PLQ54_02005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS92060.1"
                     /translation="MAGFEVRGYRCEDEEALLRAWASALPLDAIDHVTFCRKVLLDPN
                     FDPEWLIVAEADGRVVGFSLCLVRRAPLEKVGLEPHRGWITAFGVVPDSRGQGVGSAL
                     LERSVELFAREGRTEVSIAPYTPNYFVPGVDEQAYADGLRFLTARGFEVVQRPLSMDA
                     NIVKLDESPYAGRAARLAEQGIEVRTLRPHEIPLLLGFLRDHMPGDWLRHGRALLDDA
                     SRGLARFEQFTIAVHAGEILGYCQCDGDHFGPFGVRDDAQGKGIGTVLLARCLSGMRR
                     RGHHAAWVLWTSDDTASKVYSRFGFAETRRFSVLRRRLG"
     gene            complement(3018..3716)
                     /locus_tag="PLQ54_02010"
     CDS             complement(3018..3716)
                     /locus_tag="PLQ54_02010"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOS92061.1"
                     /translation="MSERPSIVAIGAHAADMEFSAGALLVKHVREGWQAHIVHLTLGE
                     KGSATLSASDYGSQKRREAEAAAQCLGCTPHFLPYADGELTPSGDVVREVAVLLRKLK
                     AKVVVAHWPGSIHGDHSAAHDVVHKAIFMAANRHFDLDGLPPAGGIRLYYADNWEDAE
                     GFEPLVYVDISEVVEDWEKGFKCYAIGRGEGGYPYWDWYQARTRLHGIKIGVSHAQAV
                     SVDRWRMIQRRDTL"
     gene            complement(3805..4977)
                     /locus_tag="PLQ54_02015"
     CDS             complement(3805..4977)
                     /locus_tag="PLQ54_02015"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS92062.1"
                     /translation="MWRVATVLVCLLVTHAGAVDDGFVPLAGGPDLRHWQTMGAGTWD
                     WQDGAIHCDGQGSGWLRSERVYEDFTLRFEYRIAPGGNSGVWLRAPAQGRQSAVGLEF
                     QIMGDVTATPDVGSTGALYDMLAPRVNAAHPAMEWNEVEISCIGPRVRAVLNGQELYD
                     VDLQDPLFNAAVPMGRRPSERLTRGFLGLTNHGSEVWYRNLRIREEPEEGFEPLFNGV
                     GLEGWRAVGDGWSAVGGLLTGRGGADQRLVLGREVENYVLRLEYRLSPGAAGAVRLRA
                     AEDSTRHRMDVVLADDAGREPGLSTSGALRGLGAPRVNAAFVAGEWNDLEIEHRDVWL
                     AVRLNGVPVLDGSIYWLSGFYRPPMRGTVSLLASTGTVEFRHMRLKALPRETRMPG"
     gene            complement(5150..>5888)
                     /locus_tag="PLQ54_02020"
     CDS             complement(5150..>5888)
                     /locus_tag="PLQ54_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92063.1"
                     /translation="PGWYPAVIDDGGAGIARCVQAADGSCSLRVREPGAVVIRLSPDI
                     LETKSVEVRRTEDVSGAGPMPVPEGPAWHVDHTGVLQLAATPGGEYTLVFPERGPEPL
                     EYAIEMDRLSGASVGTVDGTRAVWMERYQETSCEAVITRWGSPGQGADLILHAVTGDL
                     YGAQGADLVNPPARLRVFVNGQAVKELDSTAPACVPDGEPSEWGEIRVPIPAHCLRRG
                     TNTVRLVNRGPNWFAFDSLVVRMRPAQ"
BASE COUNT          993 a         2114 c         1920 g          861 t
ORIGIN      
        1 ggttcccctt gggtatgagc gcaccgaact cgacaccagg caggcggggc aggaacgcat
       61 ggatctggtc gccgaggagg cctggcaccg gctccagtcc atggggatgc accttggtga
      121 ccagcgtgct cagggccggg ggcggtcgat agcgggtgca ccgggcgaac acgcccgccg
      181 tcccggggtc gaggccgaag gcgccgatga cggcgtcggc gtggtaggtg tcgccatcgg
      241 tgtataccac gacgccgtcg tcgccgatct cgatgtcgct cgcccggtct gggacgatga
      301 gggtgccggc ccgatgggcc tcgttgagca gatgctggtc cagccgcacg cgccgcgtgg
      361 cgctcgacgc gggatcaccc ggccgtgggg gtagctcgat tcggtcctgc tcggcgtgga
      421 gcacatagcc gccgacctcg cgctggatca gacgccgggg cagtgaggtc ccccactccc
      481 ccagcacctc gtcgatctgc gagggcatca cgaccccggc gcactggttg aagtggcggc
      541 cgaagcgttt gggctcaagc aggatgacct ccaccgactc gccccgggcc tgggcgccgc
      601 accggagagc cagcgcacag gcacagccgg acggcccgcc gcccacgatg agaaccctcg
      661 acatcctcac gacggtccct ccttggggcg gtcggcagcg ccagtgtcgc gggtccgcag
      721 tccccgagtc gcgccgatgg cccagtgcca gtgaaggcgc gggctcagtg cttggcgcag
      781 cagagccgcg tatggctcat cgccggtgag caacccccag gcaatggacc gcagcggcgc
      841 cgccgagtct gactcgtctt gggccgccgc acagatcctc cgggtcaggc tggggtgccg
      901 gcccagagct tgataggcag aaaacagaat gcgaccggag cgattgtccc acacgaaggc
      961 gcgccggcag ggcttcacgt accactcgac caggctcgtt ctgtcgaccc cgccttcgat
     1021 aatggcgtgc gcggcaagct cggcggtcgt gaaggccgag tccaggccgt tcttgaggta
     1081 gcgcgagatg tgggcgtcgc cgaccaccac gattcggtcc gcgaccacat tccgggcccc
     1141 cgtgatgggc aacagcggat gacagtggca gtaccgctcg ggcatgtgaa agccttcggg
     1201 caaaagacgg cccacctcgg gctgtcgcat gaatcgctcc aggtgagctt gccccaccca
     1261 gggaccgatc agcgacacgg tcacgtggcg ctctttgggt accatcacgg ccacgatgac
     1321 gccttccatg ccgacggcca gcacgtggat gtcgctgccg aaccgccgat cgatctcggc
     1381 cgatggcagt gggatctcgc actgggccgc catggcggtt cgcggaggcg agtacccgaa
     1441 cccgagcgca cggaaggtca ggaccgtgcg gctgttcaca ccgaatgcgc cgaccaccgc
     1501 gtcagccagg tggcggccgc ctcgggcatc cgtgatgacc accgggtcgc gcgcgccagc
     1561 cggacgctcg atgccgatca caacgcggcg gatatggcgc gcgcctcctc gaaccgcggc
     1621 ggcgagcagc gcgtgatcga agctccgggc ggttccgaac tcctcgccca tgggcccgcc
     1681 gctgcgaaaa gcggtgtaga tgcgcgatcc caccggcttc tcgagccgaa ccacctggcc
     1741 gcccgagtga atgcagtagc catcaaccgc ccgctgcacc gccgacgagg ggatggagac
     1801 ccccagacgc tccagccgca cgatgagtga gttcgacacg acccccgcgc acatgttgca
     1861 cccgcgcggc cccgtgacgc cgaactcctt gcggtcgaac acggtcacat cgatggatcg
     1921 acccttctcg cgggcgagcc gcagcagatg aagggccgtc agggcacccg cgggcccgcc
     1981 accgatgatg gcaatggatc gtggctctcg aagcgtgtcc acgggcacga cccacctgcc
     2041 ggaacgccct agcccagccg ccgtctgagc acggagaacc ggcgcgtctc cgcgaacccg
     2101 aagcgtgagt agaccttgct ggccgtgtca tcactggtcc acagcaccca ggccgcgtgg
     2161 tggcctcgac gcctcatccc cgacagacac cgggcgagca gcaccgtgcc aatgcccttg
     2221 ccctgggcat cgtcccgaac accgaacggg ccaaagtggt cgccatcgca ctggcagtag
     2281 cccaggatct cgccggcgtg cacggcaatg gtgaactgct cgaagcgcgc caggccgcgc
     2341 gatgcgtcgt ccagaagggc ccggccgtgc cgaagccagt cgccgggcat gtgatcgcgc
     2401 agaaatccga gcaggagggg gatctcgtgg gggcggagcg tccgcacctc gatcccctgc
     2461 tcggccagcc gcgcggcgcg ccctgcgtag gggctctcat cgagcttcac gatgttcgcg
     2521 tccatgctca aggggcgctg aacgacctcg aaaccccgag ccgtgaggaa cctcagccca
     2581 tcggcatagg cctgttcgtc gacacccggc acgaagtagt tcggtgtgta cggcgcgatc
     2641 gagacctcgg ttctcccctc gcgggcgaag agctcgacgc tccgctcgag cagcgccgag
     2701 cccacgccct ggccccggga gtccgggacg accccgaagg ccgtgatcca gccacgatgg
     2761 ggctcgagcc ccaccttctc gagtggcgct cgccgtacca gacacaatga gaagcccacc
     2821 acacgcccat cggcctcagc cacgatcagc cattcggggt cgaagttggg gtcgagtaac
     2881 accttgcggc agaacgtcac atggtcgatg gcgtccagag ggagcgccga ggcccacgct
     2941 ctcagcaggg cctcttcgtc ctcgcaccgg taccctcgga cctcgaatcc ggccatggcg
     3001 gtggcccccg tcccgttcta cagcgtgtcc cgccgctgga tcattcgcca ccggtccact
     3061 gataccgcct gggcgtggga cacgccgatc ttgatgccgt gcagccgcgt gcgggcctgg
     3121 taccagtccc agtaaggata gcctccctct cctcgtccaa tggcgtagca cttgaaaccc
     3181 ttttcccagt cctccaccac ttcactgatg tcgacgtaga ccaggggctc gaaaccctcg
     3241 gcatcctccc agttgtcggc atagtagagg cggatgccac ccgccggggg gaggccatct
     3301 aggtcgaagt ggcggttggc ggccatgaag atagccttat ggaccacatc gtgagcggct
     3361 gagtgatcgc catgaatgct tcccggccag tgggcgacca cgaccttggc cttcagcttc
     3421 cgcaaaagga ccgccacttc gcgaaccacg tcgcccgaag gcgtgagctc cccgtcggcg
     3481 taggggagga agtgaggggt gcaacccagg cactgcgcgg ctgcctccgc ctcgcgccgc
     3541 ttctgcgagc cgtagtctga tgccgaaagt gtggcgctgc ccttctcgcc gagcgtgaga
     3601 tggacgatgt gcgcctgcca cccctcgcgc acgtgcttga caagcagggc cccggccgag
     3661 aactccatgt cggcggcgtg ggcaccgatg gcgacgatag acggtctctc gctcaaggtc
     3721 gacggcctcc taggtcattc tgcgcgcgtg tcagtaccgt ggttccaacg acccgtctcg
     3781 cgcgcaggtt ggcctgggcc gcactcaccc gggcatccgc gtctcgcgag ggagcgcctt
     3841 cagccgcatg tgacggaact ccaccgtgcc cgtcgatgcc agcagcgaga ccgtgccccg
     3901 catcggcggc cggtagaagc ctgacagcca gtagatgctg ccgtcgagaa cgggcacgcc
     3961 gttgagccgg acggccagcc acacgtcccg gtgctcgatc tcgagatcgt tccactcgcc
     4021 ggccacgaag gccgcattca cccgaggcgc cccgaggccc cggagggcgc cggaagtgct
     4081 caaccctggc tcgcggccgg cgtcatcggc gagcacgacg tccattcggt ggcgcgtgct
     4141 gtcctcggcc gctcggagac gcacggcacc ggccgctccg ggcgacaagc ggtactcgag
     4201 ccggagaacg tagttctcaa cctcacggcc gagcacgagc cgttggtcgg cacccccacg
     4261 gccggtcagc agtcccccga ccgcactcca gccgtcgccg actgcgcgcc agccctccag
     4321 accgacgcca ttgaagagag gctcgaagcc ctcctcgggc tcctcgcgga ttcgcaggtt
     4381 ccggtaccac acctcgctgc cgtggttcgt gaggcccagg aagccccggg tgagccgttc
     4441 ggacggccgc cgccccatcg gcacggcggc gttgaaaagc ggatcctgca gatcgacgtc
     4501 gtagagctcc tgcccgttaa gcaccgcgcg cacccgaggg ccgatgcacg agatctcgac
     4561 ctcgttccac tccatggccg ggtgcgcggc gttgacccgg ggcgcgagca tgtcgtagag
     4621 ggcaccggtc gagcccacgt cgggcgtggc cgtgacgtcc cccatgatct gaaactccag
     4681 accgacggcc gactgccgtc cctgggccgg ggcacggagc cacacgccgc tgttgccgcc
     4741 cggcgcgatg cggtactcga accggagggt gaaatcctca tagacccgct ccgaccgcaa
     4801 ccagccgctc ccttggccat cgcagtgaat ggccccatcc tgccagtccc acgtgcccgc
     4861 acccatggtc tgccagtgac gcagatccgg gccgcccgcc agcggcacga aaccatcgtc
     4921 gacggctccc gcgtgggtga ccagaagaca aaccagcacc gtcgcgactc gccacatggc
     4981 tctccgccct tcccgatgcc gcgcggcgag accagttcgg cctgatgcca gaccggcacc
     5041 tgcacgggcg ggctgccgcg cccctacgaa cactcagggc cccgcgcgcc gcttggaggc
     5101 agcactcgga gccctgaaag ggggtaggcc agtacccact gagaccgtgc tattgtgccg
     5161 gacgcatccg gacaaccaac gagtcaaaag cgaaccagtt cggcccccgg ttcactaggc
     5221 gcaccgtgtt ggtgccccgt cgaaggcagt gggccgggat tggcacacgg atctctcccc
     5281 actccgacgg ctcgccgtcc ggcacgcacg cgggggccgt cgagtccagt tccttgacgg
     5341 cctgtccgtt cacgaacacg cgcagccgcg ctggcgggtt cacgagatcg gcaccctggg
     5401 cgccgtagag gtcgccagtg accgcatgga ggatcagatc ggcgccctgg ccgggcgaac
     5461 cccagcgggt gatcaccgct tcacaggagg tctcctggta ccgctccatc catacggccc
     5521 gagtgccgtc gaccgtgccc accgacgctc ccgacagacg gtccatctcg atggcgtact
     5581 cgaggggctc cgggcctcgc tccgggaaca cgagggtgta ctccccgccc ggagttgcgg
     5641 caagctgcag cacaccggtg tggtccacat gccacgccgg cccctccgga acaggcatgg
     5701 ggccggcacc cgacacgtct tccgtccgcc ggacctcaac gctcttggtt tcgagaatgt
     5761 ccggactcag ccggatcacc accgcgccgg gttcgcggac ccgcagggag cacgagccgt
     5821 ccgccgcctg aacgcaacgg gcaatgcccg ccccgccgtc gtcgatgacg gccgggtacc
     5881 atccgggt
//
LOCUS       DAOSPL010000063         5846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_13816_length_5846_cov_3.607322, whole genome shotgun sequence.
ACCESSION   DAOSPL010000063 DAOSPL010000000
VERSION     DAOSPL010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13816_length_5846_cov_3.607322"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1617
                     /locus_tag="PLQ54_02025"
     CDS             <1..1617
                     /locus_tag="PLQ54_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS92064.1"
                     /translation="STVWTEINAGFTTDDTGSPTGIVGVTRDITERRRAEEALRLQSS
                     ILRSMTDLVVVTGLDGTVDYASPSVRSILGLTPEDMHGRPLTDALRAAAIDGDLVAWR
                     PGGVVPDGWRGQVRARTADGREVILEVQTSALRDENGIPLGGIWVGRDVTEVAALEAQ
                     LRQIQKLDAIGTLAGGVAHDFNNVLTGIQGHATLLKLRSEPGTPVHEAAEVIETAATR
                     GARLTQQLLGFARKGKQLNVPVDLNARVEEAIRFLGRALGKNIAISTHLTRLRAFVQG
                     DPHQLEQVIINLAINARDAMPDGGVLSFATDVVELDAAYCRAHAGARPGEYVMLSVTD
                     SGAGMPQEVQDHAFEPFFTTKEPGKGTGMGLAMVYGIVKNHGGTVRIYSEVGVGTTVR
                     IYLPVPPGEQQTGPASTELEAVVPGCESLLVVDDDDAALSSTTEMLRYLGYTVRTARS
                     GAEAIEIYRGEANAFDLVLVDLIMPGMDGRQCIRALRQINPGVRTLLATGYAMDGMAD
                     ELAREGILGLIQKPVGLARLSRAVHQALRT"
     gene            complement(1689..>1998)
                     /locus_tag="PLQ54_02030"
     CDS             complement(1689..>1998)
                     /locus_tag="PLQ54_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92065.1"
                     /translation="VLHTPRREEIDVPVRSNPVLFDGTEYAGIYEVKAGDESTRFIVN
                     ILDRRESNTKPKSEIRLASGGGPTKASTVQTNVELWRHLIMVALGVLTLEWWVYHRRV
                     "
     assembly_gap    1999..2008
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2009..3549)
                     /locus_tag="PLQ54_02035"
     CDS             complement(<2009..3549)
                     /locus_tag="PLQ54_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOS92066.1"
                     /translation="MQLHNPLNLLLLLTTIPVIALYLLRLRRRDVKVPSTLLWDRMIR
                     DVQANAPLQKLRVNLLLILQLLVLLLASLAFAAPFMKSSALSGEHQVLILDASLSMQS
                     RDVSPSRFAAAQAEALKMIDGLRRHDEMMVIEAGARTRVMCGFTSEKATLRRAVEACA
                     PRDVSSNLREAIVLTLSMSRNTSAGTRIFVFSDGAFDAIPDVQLPSNVQASFVQFGKT
                     GNNVAVTGLDFRRSLRDPSRFDLFVAVDNFGDKPVQCPLTVELDGSLLRARDSSEFTI
                     APRDRGEAVFADLAGLSGLLTARADVADDLPADNVAHAVVAGRRTLRVLLAAPGRSPF
                     LERALNADPFVKLFFGKSGDFRPGDATDYDAVVFDGDCPDEVGPGSYLLFGAAPKDSP
                     VTVTGTEERPRVTDWNPTHPITRLLPGLGSVRMSEGKTATVADWRKVLAETARTPLIV
                     AGERRGYRCVYVGFDVAKSDIVVRPAFPLLIIGALEWLTSDPTQLGERYYRAGEAVPI
                     PVAPGV"
     gene            complement(3614..4117)
                     /locus_tag="PLQ54_02040"
     CDS             complement(3614..4117)
                     /locus_tag="PLQ54_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOS92067.1"
                     /translation="MAGRAHKYGDHIDTDIIIAARYLVTADADELAQHCMEDGDAEFA
                     KRVRPGDYIVAGENFGCGSSREHAPIAIKASGIACVVAKSFARIFCRNSINIGLPILI
                     CPEAVDGTAMGDELEVALAAGRVRNVTRDREFQAQPFPAFMLEIVEAGGLIPFTRARL
                     QKAGARE"
     gene            complement(4168..5430)
                     /gene="leuC"
                     /locus_tag="PLQ54_02045"
     CDS             complement(4168..5430)
                     /gene="leuC"
                     /locus_tag="PLQ54_02045"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOS92068.1"
                     /translation="MPGMTLTEKILAAHAGRERVVPGEFVLAKVDLVLGNDVTAPIAI
                     REFESIGVDTVFAKDRIALVQSHYVPNKDIKSAEQAKVMREFARRHQIDLFWEVGRAG
                     VDHAILPQEGLVVPGDAVVGADSHTCTYGALGCFSTGIGSTDLAAAMATGECWLMVPK
                     TLRFVLTGQRRPYVSGKDIILYIIGMIGVDGALYRSMEYTGSAVADLEMHDRFTIANM
                     AIEAGAKNGVFAADAKTRAYCDGRAKREPVYLEADADAEYEKVYEIDVSQIGLQVAFP
                     HLPENARPVGEARGIRIDQVFIGSCTNGWIEDLRVAAEILRGRQVHGDVRAIVIPPTR
                     EIYQQALREGLLEVFVEAGAAVGPPTCGPCLGGHMGVLAAGEKAVATSNRNFVGRMGH
                     KDSAVYLAGPAVAAASAVAGEIASPEDL"
     gene            complement(5484..>5846)
                     /locus_tag="PLQ54_02050"
     CDS             complement(5484..>5846)
                     /locus_tag="PLQ54_02050"
                     /inference="COORDINATES: protein motif:HMM:NF020091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOS92069.1"
                     /translation="ALHLEYLHVATGMDIVPTATVRLLRNGQPVQEARTGVGSVDAVY
                     RAIAKLSGVKHQLVDFTVQSVTGGTDALADVTVRVSWRNRVFTGRGSSMDVIEASAKA
                     YVQAVNRVLLARDQAAKR"
BASE COUNT         1011 a         2049 c         1829 g          947 t
ORIGIN      
        1 tccacggtat ggaccgagat caatgcgggc ttcacgacag atgacaccgg cagccccaca
       61 ggcatcgtcg gcgtcacgcg cgatatcacc gagcgccgga gggccgagga agcccttcgg
      121 ctgcagtcgt cgatcctcag gagtatgacg gacctggtcg tcgtcaccgg actcgacggc
      181 accgtcgact acgccagccc atccgttcga agcatactcg ggttgacgcc cgaggacatg
      241 catggtcgcc ccctgaccga cgcactgcgc gcggccgcca tcgatggcga cctggtggcc
      301 tggcggcctg gcggagtcgt ccccgatggt tggcgcgggc aggtgcgcgc ccggacggcc
      361 gacgggcggg aggtcatcct cgaggtgcag accagtgccc tccgcgacga gaacgggatc
      421 ccactgggtg gcatctgggt cgggcgtgac gtgaccgagg tggcggcact cgaagctcag
      481 cttcgccaga tccagaagct cgacgccatt ggcaccctgg ccggcggtgt cgcccatgac
      541 ttcaacaatg tgctgaccgg catccagggg cacgccacgc tgctcaagct gcggtccgag
      601 cccggcacgc cggttcacga ggcggccgag gtgatcgaga ccgcggccac ccgcggcgca
      661 cggctcaccc agcagctcct gggcttcgca cgcaagggca agcagctcaa tgtgcccgtg
      721 gacctgaacg cgcgggtcga ggaggccata cgtttcctcg gccgcgccct gggcaagaac
      781 atcgccatct cgacccacct gactcgcctc cgagcctttg tgcaggggga cccgcaccag
      841 ctcgagcagg tgatcatcaa cctggccatc aatgcgcgcg atgccatgcc cgacggaggc
      901 gtgctgagct ttgccaccga cgtcgtcgag ctcgacgccg cctactgccg ggcccatgcc
      961 ggcgctcgcc ccggcgagta cgtcatgctc tccgtcaccg acagcggggc gggtatgccg
     1021 caagaagtcc aggaccatgc atttgagccc tttttcacaa ccaaagaacc gggcaagggc
     1081 acaggcatgg ggctcgccat ggtctatggc attgtcaaga accatggagg cactgtgcgg
     1141 atctacagcg aggtcggcgt cggcactacg gtccgcatct acttaccggt gccgcccggc
     1201 gaacagcaga ccggaccggc atccaccgag ctcgaagcag tggtccccgg atgcgagtcg
     1261 cttcttgtgg tcgatgacga tgacgccgcc ctgagttcga ccacggagat gctccggtac
     1321 ctgggttaca ccgtccgcac cgcccgcagc ggcgccgagg ccatcgagat ctaccgtggc
     1381 gaggccaacg cattcgacct cgtcctcgtc gatctcatca tgccaggcat ggatggcagg
     1441 cagtgcattc gcgccctgcg ccagatcaac cccggcgtcc gcaccctgct tgccaccggc
     1501 tacgcaatgg acggcatggc tgacgagctg gctcgcgagg gaatcctggg cctgatccag
     1561 aagccggtgg gcctcgcgcg cctgtcgcgg gcggtccacc aggctctccg tacgtgaaac
     1621 cgtgcggcgc gttggccatg cgggtcgcac ccggccattc cgcttggcgg aagaccagcc
     1681 atccgcccct acacgcggcg atggtagacc caccactcca gtgtcagcac cccgagagcc
     1741 accatgatga gatggcgcca cagctcaacg ttcgtctgca ccgtcgatgc cttcgtcggc
     1801 cccccgccac tcgccagccg tatctccgac ttcggcttcg tgttcgactc gcggcggtcg
     1861 agtatgttca cgatgaagcg agtggactcg tcgccggcct tcacctcgta gatgcctgca
     1921 tactccgttc catcaaacag aacggggttg gaccgcacgg gcacgtcgat ctcctcacgc
     1981 cggggagtgt gtaagacann nnnnnnnnac gcccggtgcc acggggatgg gtaccgcctc
     2041 gcctgcgcgg tagtaccgct cgcccaactg cgtggggtcg cttgtcagcc actcgagcgc
     2101 accgatgatg agcaacggga acgccggccg cacgacgatg tccgacttcg ccacgtcgaa
     2161 gcccacatac acgcaccggt aaccgcgacg ctcaccggcc acaatcagcg gggtccgggc
     2221 cgtctcggcc agcaccttgc gccagtccgc cacggtggcc gtcttgccct cactcatccg
     2281 tacgctccca agcccgggca gcagcctcgt gatggggtgc gtgggattcc agtcggttac
     2341 cctcggtcgc tcctcggtgc ccgtcaccgt caccggcgaa tccttcgggg cggccccgaa
     2401 cagcagatac gacccgggtc cgacttcgtc gggacagtcg ccgtcgaaca cgacggcatc
     2461 gtagtccgtc gcgtcgcccg gtctgaaatc cccactcttt ccgaagaaca gcttgacgaa
     2521 cgggtccgcg ttcagcgccc tctcgagaaa cgggcttcgg cctggggcgg ccagcaggac
     2581 tctcagcgtc ctgcgccccg ccaccaccgc gtgcgcgaca ttgtcggctg gcaggtcatc
     2641 ggcaacatcc gcccgagcag tgagcagacc cgagagaccg gccaggtcgg cgaacaccgc
     2701 ctcgccccga tcgcgcgggg cgatggtgaa ctcgctcgaa tcacgggcac gaagcagcga
     2761 tccgtcgagc tccactgtca gcgggcactg caccggcttg tcaccgaagt tgtccacggc
     2821 aacgaacaga tcgaaccgac tggggtcccg aaggctgcgg cggaagtcca ggcccgtgac
     2881 cgcgacgttg ttgcccgtct tgccgaactg aacgaaggac gcttgcacgt tcgaggggag
     2941 ctgcacgtcg gggatcgcgt cgaaggcgcc atcgctgaac acgaagatcc gcgtgcccgc
     3001 cgaagtgttg cgcgacatcg acagcgttag cacgatggcc tcccggagat tcgaggacac
     3061 atcgcgcggg gcacacgcct cgaccgcgcg gcgcagcgtg gccttctcgc tggtgaaccc
     3121 gcacatgacg cgcgtacgcg cccccgcctc gatcaccatc atctcgtcat gccggcgcag
     3181 gccgtcgatc atcttcaagg cctcggcctg cgcggccgcg aaccgggatg gcgacacatc
     3241 ccgcgactgc atcgacagcg acgcgtcgag gatgagcacc tgatgctcgc ccgagagagc
     3301 gctcgacttc atgaaggggg cggcgaaggc caagctggcc agcaggagca ccagcagctg
     3361 caggatcagg agcaggttca cacgcagctt ctgcaggggc gcgttcgcct gcacgtcgcg
     3421 gatcatccgg tcccacagca gagtcgacgg gaccttcaca tcgcggcgcc tcaggcgcag
     3481 caagtacagc gcgatgacgg ggatggtggt cagcagcagc agcaggttca gcgggttgtg
     3541 aagctgcatc ggcccaccac cgttcaaagc cactcagcgc agaccggtcc gatgtttggc
     3601 gcggtcccgc cccctactcg cgcgctccag ccttctgcaa ccgcgcccgc gtgaacggga
     3661 tcaggccgcc tgcctccaca atctcgagca tgaacgccgg aaacggctgc gcctggaact
     3721 ccctgtcccg ggtcacgttg cgcactcggc cggccgcgag agcgacctcg agctcatcgc
     3781 ccattgccgt cccgtcgacc gcctccgggc agatcaggat cgggaggccg atgttgatgc
     3841 tgttacggca gaagatgcgc gcgaagctct tggcgaccac gcacgcgatg ccgctcgcct
     3901 tgatggcgat gggcgcgtgc tcgcgcgagc taccgcagcc gaagttctct ccggcgacga
     3961 tgtagtctcc gggcctcacc cgcttggcga actccgcgtc gccatcctcc atgcagtgct
     4021 gcgccaactc gtccgcgtca gcggtcacca gatagcgcgc cgcgatgatg atgtcggtat
     4081 cgatatgatc tccgtacttg tgcgctcgtc ctgccatcgg gtctcccgct tcctttcgcg
     4141 cccgcagccg actgagtggt gatgaagcta gagatcctcg ggcgacgcga tctcgcccgc
     4201 cacggccgaa gcggccgcca ccgccggacc cgccaggtag acggccgagt ccttgtggcc
     4261 catgcgccca acgaagttgc ggttgctggt cgcgaccgcc ttctcgcccg ccgccaggac
     4321 gcccatgtgc ccgccaagac atggcccaca cgtcggtggg cccaccgccg cgccggcctc
     4381 gacgaagacc tcgaggagcc cctctcgaag tgcctgctgg tagatctccc gcgtcggcgg
     4441 gatgacgatg gcgcggacat cgccatggac ctggcggccg cggaggatct ccgcggcaac
     4501 ccgcagatcc tcgatccacc cattggtaca cgacccgatg aacacttgat cgatccggat
     4561 accccgggcc tcgcctaccg gcctcgcatt ctcaggcaga tgggggaagg cgacctgaag
     4621 tccgatctgg ctcacgtcga tctcgtagac cttctcatac tcggcgtctg cgtcggcctc
     4681 caagtagacg ggttcgcgtt tggcccggcc gtcgcagtac gcccgcgtct tcgcgtcggc
     4741 ggcgaaaaca ccgttcttcg caccagcctc gatggccata ttggcgatgg tgaagcggtc
     4801 gtgcatctcg aggtcggcca ccgcactgcc cgtgtactcc atcgaccggt acagggctcc
     4861 gtcgacgccg atcatgccga tgatgtacaa gatgatgtcc ttgcccgaga catagggccg
     4921 acgctggccc gtgaggacga acctcagcgt cttgggcacc atgagccagc actcgccggt
     4981 ggccatggcg gcggccagat cggtactgcc gatcccagtc gagaagcacc ccagggcccc
     5041 ataggtgcag gtgtgcgaat cggcacccac gaccgcatcg ccagggacca ccagcccctc
     5101 ctgcggcagg attgcgtggt cgacgccagc cctgccgact tcccagaaca gatcgatctg
     5161 gtgccgtcgc gcgaactccc tcatcacctt ggcctgttcg gccgacttga tgtccttgtt
     5221 cggtacatag tggctctgaa ccagggcgat acggtccttg gcgaacaccg tgtccacacc
     5281 aatggactcg aactcccgga tggcgatggg ggcggtgaca tcattgccga gcacgaggtc
     5341 cactttggcc aggacaaact cgcccggcac cacgcgctct cggccggcgt gggccgccag
     5401 gatcttctca gtcaaggtca tcccgggcat agcggtatcc ttccgagtag ggtggtggaa
     5461 ccggcgatag gctcctgtgg gctttatcgc ttggccgcct ggtcccgcgc aaggaggacg
     5521 cggttcactg cctgcacgta ggccttggcg ctggcctcga tcacgtccat gctcgagccg
     5581 cgaccggtga agacgcggtt ccgccacgac acacggaccg tcacgtcggc cagcgcatcc
     5641 gtgccgcccg tcacggactg gaccgtgaag tccacgagct ggtgcttcac accggacagc
     5701 ttggcgatcg ctcggtacac cgcgtccacc gacccgacgc cagtgcgcgc ctcttggacc
     5761 ggctgaccat tccgcagcag ccggaccgtg gccgtcggca cgatgtccat tcccgtcgcc
     5821 acgtgaaggt actccagatg cagcgc
//
LOCUS       DAOSPL010000064         5832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_13853_length_5832_cov_3.325255, whole genome shotgun sequence.
ACCESSION   DAOSPL010000064 DAOSPL010000000
VERSION     DAOSPL010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13853_length_5832_cov_3.325255"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PLQ54_02055"
     CDS             <1..352
                     /locus_tag="PLQ54_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92070.1"
                     /translation="LRDRAGGLPSRGPNPTLQLPSAWGGAVTVEPLKQSAVWWCIGRH
                     DFRANQPANACAEMGYARIETGPEADRDVIRENGLTIAMTGGHGALTGGLIRRADHDP
                     IEALRAADCMFSVT"
     gene            376..1140
                     /locus_tag="PLQ54_02060"
     CDS             376..1140
                     /locus_tag="PLQ54_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOS92071.1"
                     /translation="MEPIRQCASWGCFCRDGVTPQQLVTEAAKIGYAALEMPPTEHYD
                     LIREHGLLIGTIVGHASLADGLNKRENHDRIEGEILANLDVAVRYGIPNLIVLSGNRE
                     GKSEIEGAENTIAILSRVAKPAEEKGVNLVLELLNSKVDHHDYQCDSTSWGAHVVRMV
                     GSPRVKLLYDIYHMQIMEGDIIRTIRDHIDLIGHFHTAGNPGRRDLDDAQEINYRAVA
                     RAIAETGYRGLVGHELIPKGDPLECLRASFEVFRVA"
     gene            1195..2625
                     /locus_tag="PLQ54_02065"
     CDS             1195..2625
                     /locus_tag="PLQ54_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOS92072.1"
                     /translation="MTLVPVAAILAQAGPFEPTWASLEQYSVPEWYRDAKFGIFIHWG
                     VYSVPAFGNEWYPRNMYREGSDEYRHHVEVFGSHKRFGYKDFIPQLTAERFDPVEWAD
                     LFAQAGARYVVPVAEHHDGFAMYDTALSDWCAAKMGPRRDIVGELATAVRERGMVFGL
                     SSHRAEHWWFFNGGMTFDSDVRDPANAGLYGPAQPEGTQPDETFLNDWLARSCELVDR
                     YRPQVVWFDWWIEQPAFEPYLREFASYYYNRAAEWGVGVAINYKNQAFPVRAAVYDVE
                     RGQLGDIREPFWQTDTSVSRNSWGYVANQDYKTAESVIHDLVDIVSKNGCLLLNIGPK
                     PDGTIPDPEREILLQIGEWLRVNGEAIYGTRHWRVFGEGPTHVVGGSFSDTKRAPFTS
                     ADFRFTAQGDALYAICMGWPEREFVIRSLSPNLALYLDDVADVTMLGSAETLGWSRDD
                     AGLHVTAPATRPCDFAYVLKITRKTP"
     gene            2661..4151
                     /locus_tag="PLQ54_02070"
     CDS             2661..4151
                     /locus_tag="PLQ54_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOS92073.1"
                     /translation="MDRPNILIFMTDQQRGDTALAGSPAHMPNLARMRSEGVTFSNAF
                     CPAPHCCPSRATFHTGLYPSEHGVWNNVDVGNALSRGLAPGTRLWSDDLREAGYALSF
                     SGKWHISAVETPADRGWQMGFPEPRPTVSAEEVASGRPHTREWAMYERLAGQPEAAVR
                     HEGEILRPGYGTYTHYGTSPHAIVEFDEHTVEDAFRVIHGRRVSEGPWCQFVGTLGPH
                     DPYFAPADFVGRYDPGDVRLPDNFGDRMEDKPALYRRTRDRFDQLSEREHREALRHYL
                     AFCSYEDALFGRLLGALEERGELDRTLVIYLSDHGDYGGEHGLWCKGLPCFRSAYHVP
                     AVIRWPDGIREPGRVVDAFVSLADFAPTLLEAAGIGAGRPLSGQSLMPFLRDEPPAQW
                     RDALFTQTNGNELYGIQRSVMTREWKYVYNGFDYDELYDLRNDPGETRNVIGDPAHAD
                     VVRELCARMWEFAHAHRDVCINPYIMVAHAPVGPAAAFGEFRRHGT"
     gene            complement(4190..4960)
                     /gene="pstB"
                     /locus_tag="PLQ54_02075"
     CDS             complement(4190..4960)
                     /gene="pstB"
                     /locus_tag="PLQ54_02075"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOS92074.1"
                     /translation="MEEPTVEPKIAVRGLDLYYGRFHALKNIDLDVRPNTITAIIGPS
                     GCGKSTLLRTFNRMNDLITGVRVDGSVRLDGQDIWTGSLTPVQLRRRVGVVFQRPNPF
                     PLSVFDNVAFGPRAHGQRNRRVLEELVEESLRGARLWDEVRDRLNQPATELSLGAQQR
                     LCVARLLAVRPDVILLDEPCSALDPVSTLAIESLMRELQDRYTIVIVTHNMQQAARAS
                     DESVYMLLGEIIEHGPTSQVFTNPKDSRTEQYITGRLG"
     gene            complement(4964..5782)
                     /locus_tag="PLQ54_02080"
     CDS             complement(4964..5782)
                     /locus_tag="PLQ54_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HOS92075.1"
                     /translation="MTREFSYSLEAGGRAAESVPSEPKLLVERLSVSYDGVAALRSVN
                     LSIYPNEVLALTGPGGSGKTTLLRTINRLNDRIRSARVTEGRVLLDGEDVYGPDVDAY
                     AIRQRMAMVFALPTPLPKSIFDNVAYGPRVAGIRKMARLREIVERALRDAILWDEVSS
                     RLGDPAMTLSGGQQQRLCIARALAQQPEVLLLDEPCSGLDPISTLKIEDALRKLKEQY
                     TIVLVSHNTQQAARVADRIAFLLMGELVEHGRADQVVTSPADTRTADYFQGRFG"
BASE COUNT         1009 a         1945 c         1862 g         1016 t
ORIGIN      
        1 cctgagagac cgcgcgggag gtctgccatc ccgcgggccg aaccccacgc tccagttgcc
       61 ctcggcctgg ggaggggcag tcaccgtgga gccgctcaag caatcggctg tctggtggtg
      121 catcggtcgc cacgacttca gggccaacca gccggcgaat gcatgcgccg agatgggcta
      181 cgcccgcatc gagacgggcc cagaagccga ccgcgacgtg atccgcgaga acggcctcac
      241 catcgccatg accggcggcc atggggcact caccggcggc ctgatccgcc gggcggacca
      301 cgaccccatc gaggcccttc gggcagccga ttgcatgttc agcgtcacgt agagcagcag
      361 gaggggactg gacctatgga gcctatccgc cagtgtgcct cgtgggggtg cttctgccgc
      421 gacggcgtca caccgcagca gctcgtgacc gaagccgcga agatcggcta tgcggcgctc
      481 gagatgcctc cgaccgagca ctacgacctg attcgcgagc acggcctact gatcggcacc
      541 attgtcggcc acgcctctct ggccgatggg ctcaacaagc gcgagaacca cgatcgcatc
      601 gagggcgaga tcctggcgaa tctcgacgtc gcggtgaggt acggtattcc gaacctcatc
      661 gtgctcagcg gcaaccggga gggcaagtct gagatcgagg gtgcggagaa caccatcgcg
      721 atcctctcga gggtggccaa gcccgccgag gagaagggtg tcaacctcgt gctcgagctg
      781 ctcaacagca aggtcgacca ccatgactac cagtgcgaca gcacctcttg gggtgcccac
      841 gtggtccgga tggttggatc cccgcgtgtg aagctgctgt acgacatcta ccacatgcag
      901 atcatggagg gcgacatcat ccgcaccatt cgagaccaca ttgacctgat cggccacttt
      961 cacacggcag gaaatccggg gcgccgcgac ctggacgatg cccaggagat caactatcgc
     1021 gcagtggccc gcgccatcgc ggagacggga taccgtggcc ttgtgggcca tgagctgatt
     1081 cccaagggcg atcccctcga gtgtcttcga gcatccttcg aggtgttccg cgtcgcatga
     1141 gacgtgggat gcagagcccg agaggtgaga ccctctgcta cgaggaggca tgtgatgacg
     1201 ctggtgccgg tcgcagccat actggcacag gccgggccct ttgagccgac ctgggcctcg
     1261 ctcgagcagt actcggtccc tgaatggtac cgggacgcca agttcggcat cttcatccac
     1321 tggggtgtct actcggtacc ggcattcggc aacgagtggt acccgaggaa catgtaccgc
     1381 gaggggtccg acgagtacag gcaccacgtg gaggtcttcg gctcgcacaa gcggttcggc
     1441 tacaaggact tcatccctca actcacggcc gaacgcttcg atccggtcga atgggccgac
     1501 cttttcgccc aagccggcgc gcgctacgtc gtccccgtgg ccgagcacca cgatggcttc
     1561 gccatgtacg atactgcgct atctgactgg tgcgccgcca agatgggccc gaggcgagat
     1621 atcgtcggag agctggccac agccgttcgc gagcgcggta tggttttcgg gctatcatcg
     1681 caccgggcgg agcactggtg gttcttcaat ggcgggatga cgttcgactc cgatgtgcgt
     1741 gacccggcga atgccggtct gtacgggcca gctcagcccg aggggaccca gcctgatgag
     1801 acgttcctca atgattggct agcccgatcg tgcgagctcg tcgaccgcta ccggcctcag
     1861 gtcgtctggt tcgattggtg gatcgagcaa ccggccttcg aaccttacct gcgcgagttc
     1921 gcctcctact actacaaccg ggctgccgag tggggtgtgg gggtcgccat caactataag
     1981 aaccaggcat tccccgttcg ggcggccgtc tacgacgtcg agcgtggcca gcttggtgac
     2041 attcgcgagc ccttctggca gacggacacg tcggtatccc gcaactcatg gggttacgtg
     2101 gcaaatcagg actacaagac ggccgaatcg gtgatccacg atctggtgga catcgtcagc
     2161 aagaacgggt gtctgctgct gaacattggt ccgaagcccg atgggaccat ccccgatccc
     2221 gagcgggaga tcctgctgca gatcggcgag tggctccgcg tcaatggcga ggccatctac
     2281 ggcacacgtc actggcgggt gtttggcgag ggaccgaccc acgttgtcgg cggcagcttc
     2341 agcgacacca agcgcgcccc gttcacttcc gccgatttcc gcttcaccgc ccagggagac
     2401 gcgctctatg ccatctgcat gggctggcct gaacgcgagt tcgtgatccg ctcgctcagc
     2461 cccaacctgg ccctgtacct ggacgatgtg gccgatgtga cgatgctggg ttcggccgag
     2521 accctcggct ggagccgaga cgacgcgggc ctgcacgtca ccgccccggc cactcggccg
     2581 tgtgacttcg cctatgtcct gaagatcaca cggaagactc cgtagcggcg gccggactgc
     2641 tgcccgggca gagagggatc atggaccgtc cgaacatcct gatcttcatg accgatcagc
     2701 agcgtggcga caccgctctg gccggctcac cggcgcacat gccgaacctg gcgcgcatga
     2761 ggagtgaggg cgtcaccttc tcgaacgcct tctgccccgc gccccactgc tgtccgtcgc
     2821 gtgccacctt tcatacgggc ctgtacccgt cggagcatgg tgtctggaac aacgtggacg
     2881 tgggcaatgc gctctcccgc ggcctggccc caggcacgcg tctgtggagc gacgacctgc
     2941 gcgaagccgg ctacgctctc agtttctcgg gcaagtggca catcagcgcg gtcgagacac
     3001 cagcggaccg tggctggcag atggggttcc ccgaaccccg gccaaccgta tcggccgagg
     3061 aggtggcgtc gggccgtccg cacacccgtg agtgggcgat gtacgagcgc ctggcaggcc
     3121 agcctgaagc ggccgttcgc cacgaggggg agatccttcg tccaggctat ggcacctaca
     3181 cgcactacgg gacctcgccg cacgccatcg tcgagttcga cgagcacacg gtggaggacg
     3241 ctttccgcgt gatccatggt cgtcgggtct cggaggggcc ctggtgccag ttcgtcggca
     3301 ctctgggccc gcacgatccc tacttcgcgc cggcggactt cgtcggacgc tatgaccccg
     3361 gtgatgtacg ccttcccgac aactttggtg accggatgga agacaagccg gccctgtacc
     3421 gccgcacgcg cgaccggttc gatcagctct ccgaacggga gcatcgtgaa gccctgcggc
     3481 actacctcgc gttctgcagc tacgaggatg ccctgttcgg ccggctgctt ggcgcactcg
     3541 aggagcgcgg cgagctggat cgcacgctcg ttatctacct ttcggaccac ggcgactacg
     3601 gcggcgagca cggcctgtgg tgcaagggat taccgtgctt ccgctcggcc taccatgtgc
     3661 ctgcggtgat ccggtggcct gatggcatcc gcgagcccgg gcgcgtcgtc gacgccttcg
     3721 tctccctcgc cgactttgcg cccacgctgc tggaggccgc gggcatcggt gcgggccgac
     3781 ccctgtccgg ccagagcctc atgcccttcc tgcgcgatga gccacccgct cagtggcgcg
     3841 atgcgctgtt cacccagact aacggcaacg agctctacgg aatccagcgg tcggtgatga
     3901 cgcgcgagtg gaagtacgtc tacaacgggt tcgactacga cgagctgtac gatctgcgca
     3961 atgacccggg cgagacccgg aacgtcatag gagatcccgc ccacgccgac gtcgtccggg
     4021 agctctgcgc gcgcatgtgg gagttcgccc atgcccacag ggatgtgtgc attaaccctt
     4081 acatcatggt cgcccacgca ccggtggggc ccgcggcggc gtttggtgag ttccggcgac
     4141 acggtaccta accaccaagg cgcgcagtta ggcatggtgt cccccagact caccccagcc
     4201 ttccggtgat gtactgctcg gtccgggaat ccttcggatt ggtgaagacc tggctcgtgg
     4261 gtccatgctc gatgatctcg cccagcagca tgtacacgct ctcgtccgac gcgcgagccg
     4321 cctgctgcat gttgtgggtg acgatcacga tcgtgtaccg gtcctgaagc tcacgcatga
     4381 ggctctcgat ggccagcgtc gagactgggt cgagcgccga gcacggctca tcgagcagga
     4441 tcacgtcggg tcgcaccgcc agcaacctgg cgacgcacaa cctctgctgg gcgcccagcg
     4501 agagctcggt cgccggttgg ttgagccggt cgcgcacctc gtcccatagc cgggctcccc
     4561 gcaggctttc ctcgacgagc tcctccagca cccgccggtt ccgctgtcca tgggcccgcg
     4621 ggccgaacgc cacattgtcg aacaccgaaa gcgggaacgg gttcggacgc tggaaaacca
     4681 cgccgacccg tctgcgcagc tgcacgggcg tgaggctgcc cgtccagatg tcctggccgt
     4741 cgagccgcac cgagccatca acccgcacgc ccgtgatcag gtcgttcatc cggttgaagg
     4801 tgcgaagtag cgtcgacttc ccacaccccg aaggcccgat gatcgccgtg atggtgttcg
     4861 ggcgcacgtc gaggtcgatg ttcttcagcg cgtggaagcg gccgtagtac aggtccagtc
     4921 ctcgcacggc gatcttgggc tcaacggtgg gctcctccac gggctaacca aacctcccct
     4981 ggaagtagtc cgccgtgcgc gtgtcagccg ggctggtcac aacctggtcg gcgcgtccgt
     5041 gctcgaccag ctcgcccatc agcaggaacg cgatccggtc ggccacccgt gccgcctgct
     5101 gcgtgttatg gctgaccagc acgatcgtgt actgctcctt gagtttccgc aaagcgtcct
     5161 cgatcttcag tgtcgagatg gggtccagtc cagaacatgg ctcatccagg agcagcactt
     5221 cgggctgctg ggccagcgcc cgcgcaatgc acaaccgctg ctgctgtccg cccgagaggg
     5281 tcatggcagg atcacccagc cggctgctga cctcgtccca caggatggca tctcgcagtg
     5341 cgcgctcgac gatctcgcgc aaccgcgcca tcttgcggat tcccgccacc cgcggaccgt
     5401 aggccacatt gtcgaagatg ctcttgggga gaggcgtcgg cagagcgaag accatggcca
     5461 tgcgctgacg gatggcgtac gcgtccacgt cgggcccgta cacgtcctca ccatcgagaa
     5521 ggactcggcc ttcagtcaca cgggccgatc ggatccggtc gttcaggcgg ttgatcgtcc
     5581 gcagcagcgt cgtcttccca ctgcctccag ggccggtgag cgcgagcacc tcgttcgggt
     5641 agatcgagag gttcaccgac cggagcgcgg ccacaccgtc ataggacacg gagagccgct
     5701 caaccaagag cttgggctcg gacgggacgg actctgccgc cctgcctccc gcctccaggc
     5761 tgtagctgaa ctcgcgtgtc atggccgcgc ggacaccgcc agtctccccc tagccctgct
     5821 tcgcctggaa gc
//
LOCUS       DAOSPL010000065         5823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_13880_length_5823_cov_2.933252, whole genome shotgun sequence.
ACCESSION   DAOSPL010000065 DAOSPL010000000
VERSION     DAOSPL010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13880_length_5823_cov_2.933252"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="PLQ54_02085"
     CDS             <1..1055
                     /locus_tag="PLQ54_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOS92076.1"
                     /translation="PWPKFRGNRANTGLSPSIGAQAPTLKWLYAVPNVRSSPCVSADG
                     TVYVGGSDSNFYAINGATGVLRWTFTTPTSEINSDPAIGRDGTIYIGSSDTSMYAVYQ
                     ASGTQRWAHATSAAGLIQSGAAIGADGTIYVGAQDGVLYALSGTTGAEQWTFDSGGSY
                     RAIPAIGADGTIYVGSDSNVFYALNPSTGAEVWHFDAGGDIGGSAAIGADGTVYFGSY
                     DANVYALDGTTGAEKWHFTTGGSVYGSPGVGADGTIYIGSLDNNVYAIDSATGTEKWR
                     YTTGGDVDSSPAIGADGTVYIGSDDGKLYALNGATGAARWIYTTGGQIHSSPAIGADG
                     TIYFGSEDDYLRALGP"
     gene            complement(1065..1622)
                     /locus_tag="PLQ54_02090"
     CDS             complement(1065..1622)
                     /locus_tag="PLQ54_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92077.1"
                     /translation="MRRFVLAVAGMVVIQVACSCAKRGEEPPAQPQPASVAEPAQPAA
                     RGLGAAKGAAEPAVEPAPAANAASAGAKCVMNLSYLSKALREYTKAHGGKLPQVASAD
                     ELAKALAPYLRVDESASFTCPETGKPYQFNAALAGKRASSVGPELVLKDSVPHDSGDV
                     NAVTVGGTLGSHAASDAALAKGTFR"
     gene            1753..3246
                     /locus_tag="PLQ54_02095"
     CDS             1753..3246
                     /locus_tag="PLQ54_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HOS92078.1"
                     /translation="MGRREAVGTMIRVAMIGAGSIGFTRRMMMDILAVPELVGTEFRL
                     MDINAEALEMSASLCRKMIDDAHLPATVVATPDRREALRDADFVFSFVRVGGLEAFAH
                     DIEIPLKYGVDQCVGDTLGPGGIFYALRTIPVLVGIAKDMEDVCPRALLLNYSNPMAM
                     NCWGVRRASKTPMVGLCHGVQGAHWQIALALGLPVEEVEFVAAGLNHQTWFIQVRHKG
                     KDMLPFVLDAFERNESLAHSEAVRIDVMRRFGYYSTESNGHLSEYLMWYRKRPQELKS
                     RWICPDTWIGGRTGGYLEECRKTADHYRQMYPKWLSGEADAIRLGDRSSEHGSYIVEA
                     LVTGRRYRGHFNVENTGLITNIPNGCTIEIPCMVDANGIAPTYIGDLPLGCAAILRQT
                     ISVQELTVEAALTGDKELVRQALALDPLTGSVCNLDEVWAMADEMLEALSPWLPQFAE
                     GRQSPRRAPQPGLRMTRPIGSPPGSATVDAAGALEKTFALRGGGDRG"
     gene            complement(3353..4381)
                     /locus_tag="PLQ54_02100"
     CDS             complement(3353..4381)
                     /locus_tag="PLQ54_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92079.1"
                     /translation="MIRTLACWLPILCAALAGSRAVAVEMTAEVWKRQVLPEEPILVR
                     VTLTNPTDYTVSVVPPYLAGPSFPLRVVEVRDDDQRRQPEYRHNNRGSWLWVPIGGEW
                     QRSKRRPWVLGPGEAAWTWHNLATFCELHEPGRHHLVLQYEPQPSMLRISGDETSPLP
                     DNLWCAALEVDLGWIEILEPPEAERPLVEKLAARSYACWPKGEIPDRSLAPEVAAEAP
                     TSVYVPYLEFYSMVARTRHSLMRPDRLERLRAEVRAFRERNPGFPLDYQLDVLLARCE
                     YFSAIGEVVFARDHRLTPTAEQLARVPPLAQALREAAAASGDAGLVCEMEAWIGLAER
                     QYHLGEGG"
     gene            complement(4393..>5823)
                     /locus_tag="PLQ54_02105"
     CDS             complement(4393..>5823)
                     /locus_tag="PLQ54_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92080.1"
                     /translation="AVRIETQVTEVWPPPAAGGPEAGGHVYISDTLSQFPNAPILFRR
                     PLGDAVRCQTVDHQANIEALVELRITNTDQSTSTNATCGGMQVYFRSLDPDSGTLWDT
                     TPGLAGDDNVDTSVGEGRGTLAPQVSTAAANQSGDFPARTAAVATTTLTFTDHQAGDN
                     YVVEASTCDLAVTEPPWVASGVLDAWKLIWYERDRMFKEGSFLSAAAAAGATTVTVEA
                     ADPAWVGRTAAVFDPDWQDINDTHLVTNVAGNTVTLDPPLTHNCRADGVGALGIIRTV
                     GDSRCDALETMLLPRSFGPGEAFVEWRERPAPEHDSGLYPYFPVADVEAPWPPYFRYA
                     GEPNTVHFIDGHHCTAAGYEGRVGLAGGFEVMMFQYASGDEAMTLDRTQLAEQLVHEF
                     GHMFGLEDFNFDGGGGHPDHLNHASTDGCIMLYTIAGAPFNPTHPHTTGSASALFGGP
                     TSPPAEPVDLILVAGRAEPLPGAPGG"
BASE COUNT          937 a         2094 c         1855 g          937 t
ORIGIN      
        1 cgccctggcc caagttccgc ggcaaccgcg ccaacaccgg gctcagccca agcatcggcg
       61 cgcaggcccc cacattgaag tggctctatg ccgtcccgaa cgtccgcagc agcccctgcg
      121 tcagtgccga cggcacggtc tacgtcggcg gctctgacag caacttctac gccatcaacg
      181 gcgcgaccgg agtgctaagg tggaccttta cgacgcccac cagcgagatc aactccgacc
      241 ccgccatcgg cagagacggg accatctaca tcggaagctc ggacaccagc atgtacgccg
      301 tgtaccaggc gtcgggcacc cagaggtggg cgcacgccac atcggcagcg ggcctcatcc
      361 agagcggcgc ggccatcggt gccgacggca ccatctatgt cggcgcccaa gacggcgtcc
      421 tctatgccct gtccggcact accggagccg agcagtggac cttcgactcc ggcggctcct
      481 accgcgccat ccccgccatc ggtgccgacg gcaccatcta cgtcggcagc gacagcaacg
      541 tcttctatgc cctcaacccc agcaccgggg ctgaagtgtg gcacttcgac gccggcggcg
      601 acatcggtgg cagcgcggcc atcggcgcag acggcaccgt ctacttcggc agctacgacg
      661 cgaacgtcta tgccctcgac ggcaccaccg gcgcggagaa gtggcacttc accaccggtg
      721 gcagcgtata cgggagcccc ggagttggcg cggatggcac catctacatc ggcagcctcg
      781 acaacaacgt ctacgccatc gattcggcta ccgggaccga gaagtggcgc tacaccacgg
      841 gcggcgacgt cgacagcagc ccggccatcg gcgccgatgg caccgtctac atcggcagtg
      901 acgacggcaa gctctacgcc ctcaatggcg ccaccggagc cgccaggtgg atctacacca
      961 cgggcggcca gatccacagc agcccggcca tcggggcgga tggcaccatc tacttcggaa
     1021 gcgaggacga ctacctgcgt gctctcgggc cgtagggcgc ccaactaccg aaacgtgccc
     1081 ttcgcgagcg cggcatcgct ggccgcgtgc gatcccagcg tgccgcctac cgtgactgcg
     1141 ttgacgtccc cgctgtcgtg gggcaccgag tccttcagca ccagctccgg ccccaccgag
     1201 ctcgccctct tgcccgccag cgcggcgttg aactggtacg gcttccccgt ctccgggcaa
     1261 gtgaacgagg ccgactcatc gaccctcagg tatggggcca gagccttggc cagctcgtca
     1321 gcactcgcca cctggggcag tttgccgccg tgggccttcg tgtactcgcg aagcgccttc
     1381 gacaggtagg acaggttcat cacgcacttg gccccggccg atgccgcatt ggcggccgga
     1441 gccggctcca cggcgggctc tgccgccccc ttcgccgcgc cgagtcccct cgcggccggt
     1501 tgggcgggct cggccaccga tgccggctga ggctgcgccg gcggctcctc gccccgcttc
     1561 gcgcacgagc aggccacctg tatgacaacc ataccagcta cagccagcac gaaccgccgc
     1621 acgtaacccg cctcccgtac acttcagcca acccgacccc gcctgccttc gacgccgacg
     1681 ccccgcgccc ttcccccttg cccccactcc cctcagcccc cacaatgtgc gccgtccgga
     1741 aggacggcat cggtgggacg tcgagaggcc gtggggacca tgatccgtgt cgcgatgatc
     1801 ggggcgggga gcatcggctt cacccgccgc atgatgatgg acattctggc cgtgccggag
     1861 ctggtcggta ccgagttccg cctgatggac atcaatgccg aagccctcga gatgtccgcc
     1921 agcctgtgcc ggaagatgat cgacgatgcc catctgccgg ccacggtcgt cgcgacgccc
     1981 gaccggcgcg aggccctgcg cgatgccgac ttcgtcttct cattcgtccg agtgggcggg
     2041 ctcgaggcct tcgcgcacga tatcgagatc cccctcaagt acggcgtcga tcagtgtgtg
     2101 ggcgataccc tcgggccggg cggcatcttc tacgctctac ggaccatccc ggtactggtc
     2161 ggcatcgcca aggatatgga agacgtctgc ccgcgggcac tactcctcaa ctactcgaac
     2221 cccatggcca tgaactgctg gggcgtgcgc cgcgcgagca agacaccgat ggtcggcctg
     2281 tgccacggcg ttcagggcgc gcattggcag atcgcgctgg ccctcgggct cccggtcgag
     2341 gaggtcgagt tcgttgctgc cggcctcaat caccagacgt ggttcatcca ggtgcgccac
     2401 aagggcaagg acatgctgcc cttcgtgctc gacgccttcg agcggaacga gagcctggcc
     2461 cactcggaag cggtgcgcat cgacgtcatg cgccggtttg ggtactactc gaccgagtcg
     2521 aacgggcacc tttctgagta cttgatgtgg taccggaagc ggccccagga gctgaagagc
     2581 cggtggatct gccccgacac atggatcgga ggccgcaccg gtggctacct cgaagagtgc
     2641 cggaagacgg ccgaccacta tcgccagatg taccccaagt ggctctccgg cgaggccgac
     2701 gccatccgcc ttggcgaccg gtcatccgag cacggctcct acatcgtcga ggcactggtc
     2761 accggccgtc gttaccgcgg gcatttcaat gtcgagaaca cggggctgat caccaacatc
     2821 ccgaacgggt gcaccatcga aatcccgtgc atggtcgacg cgaacggtat tgcaccgacg
     2881 tacatcggcg atctcccgct cggctgtgcc gccatcctcc gccagaccat ctcggtgcag
     2941 gagctcacgg tcgaagcggc cctcacgggc gacaaggagc tggtccgtca ggccctggct
     3001 ctcgatccgc tcaccggctc cgtgtgcaac ctcgacgagg tctgggccat ggccgacgag
     3061 atgctcgagg cactctcgcc ctggctgccg cagttcgcag agggtcgcca gagcccgcgc
     3121 cgggcgccgc agccgggcct ccggatgacc agacccatcg gcagtccgcc aggctcggcc
     3181 acggtcgacg ccgccggggc gctggagaag acatttgcgc tgcgtggggg cggggaccgg
     3241 gggtaggcac tcgactgatg gtgtcatcgc gcccgccatc cagtgacaca ggcgtcccgc
     3301 ctgagagtcg gcggagccat cgcccgcggg gttgccccca tgcttgctct ggctagccac
     3361 cctcccccag gtggtactgc cgttcggcca ggccgatcca cgcctccatc tcgcagacca
     3421 gccccgcgtc gcccgacgcg gcagccgcct cgcgcagcgc ctgcgccagc ggcggaaccc
     3481 tcgccagttg ctccgcggtc ggtgtcagac ggtggtccct ggcgaaaacg acctccccta
     3541 tggccgagaa gtactcgcat cgcgccagca gcacatccaa ctggtagtcc agcgggaagc
     3601 cgggattgcg ctcgcggaat gctcggactt ctgcgcgtag gcgctcgagc ctatcgggcc
     3661 gcatgaggct gtgcctcgtt cgggcgacca tcgagtagaa ctcgaggtag ggcacgtaga
     3721 ccgacgtcgg cgcctcggcc gccacctcgg gggcgagcga gcgatctggg atctctccct
     3781 tgggccagca ggcgtaggac cgcgccgcga gcttctcgac cagcggacgc tcggcctcgg
     3841 gcggctccag gatctcgatc caccccaggt caacctcaag ggccgcacac cacaggttgt
     3901 cgggcagggg cgaagtctcg tcgccgctga tccgcagcat cgacggctgc ggctcgtact
     3961 gcagcaccag gtggtgcctc cccggctcgt gcagctcgca gaacgtggcc aggttgtgcc
     4021 acgtccacgc cgcctcgccc ggcccgagta cccaaggcct tcgcttcgac ctctgccact
     4081 cgccgccgat cggcacccat agccagcttc cgcggttgtt gtgccggtac tccggctggc
     4141 gccgctgatc gtcgtcgcgg acctcgacca ctcggagggg gaagctgggg ccggccagat
     4201 acggtggtac gacgctgacc gtgtagtctg tcgggtttgt cagtgtcacc cgcaccagaa
     4261 tgggctcttc gggcagcacc tgccgtttcc agacctcggc cgtcatctcg acagcgacgg
     4321 cgcgcgagcc cgcgagggcc gcacacagga tcgggagcca gcatgctagg gttcggatca
     4381 tggcagccac ggttatcccc ccggtgctcc gggcaggggt tccgccctgc ccgctaccag
     4441 aatcaggtcc accggctcgg ccggcgggct cgtgggtccg ccaaagaggg cactcgccga
     4501 cccagtcgtg tgtgggtgcg tcgggttgaa tggtgcccca gcgatggtgt acagcatgat
     4561 gcacccgtcg gtgctggcat ggttgaggtg gtcaggatgg cccccgcccc catcgaagtt
     4621 gaagtcctcc agcccgaaca tgtgcccgaa ctcgtgcacg agctgctcgg ccaactgcgt
     4681 gcggtccagt gtcatcgcct cgtcgccgga cgcgtactgg aacatcatca cctcgaagcc
     4741 ccctgccagg ccgaccctcc cttcataccc ggccgccgtg cagtggtggc catcgatgaa
     4801 gtggaccgtg ttgggctccc ccgcgtagcg gaagtacggc ggccaggggg cttccacgtc
     4861 cgccaccggg aaatacgggt acagccccga atcgtgctca ggtgcgggac gctcacgcca
     4921 ctcaacgaat gcctcgcccg ggccgaacga gcgggggagc agcatcgtct ccagtgcatc
     4981 acacctcgag tcccccactg tccggatgat gcccagcgca cccacgccgt cggcacggca
     5041 gttgtgcgtc agtgggggat ccagtgtgac agtgttgccc gccacgttcg tgaccagatg
     5101 cgtgtcgttg atgtcctgcc agtccgggtc gaagaccgcg gcagttctcc cgacccaggc
     5161 cgggtccgcc gcctccacgg tcaccgtcgt cgcccccgcg gcagcagcag cggacaggaa
     5221 ggagccctcc ttgaacatgc ggtctcgctc gtaccagatc agcttccacg catccagcac
     5281 gccgctagct acccacggcg gttcggtgac ggccaggtcg cacgtgctcg cctcgaccac
     5341 gtagttgtcc cccgcctggt ggtcagtgaa cgtcagcgtt gttgtcgcca ccgccgcggt
     5401 ccgcgccggg aagtcgccgc tctggttggc ggccgcggtg ctcacttggg gtgccaacgt
     5461 tcccctcccc tcgccgaccg acgtgtccac gttgtcgtct cccgccaggc ccggggtggt
     5521 gtcccacaac gtcccgctgt ccggatccag cgagcggaag tagacctgca tccccccgca
     5581 ggtcgcgttg gtgctggtgc tttgatccgt gttcgtgatc ctgagttcca ccagcgcctc
     5641 gatgttcgcc tggtggtcga ccgtctggca acggacggca tcgcccagcg gtcgccggaa
     5701 caggatcggt gcgttcggga actggctaag ggtgtcactg atgtacacgt ggccaccggc
     5761 ttcagggccg cccgcagcgg gcggtggcca gacctcggtg acctgggtct cgatgcggac
     5821 ggc
//
LOCUS       DAOSPL010000066         5809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_13932_length_5809_cov_3.930831, whole genome shotgun sequence.
ACCESSION   DAOSPL010000066 DAOSPL010000000
VERSION     DAOSPL010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13932_length_5809_cov_3.930831"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            373..1497
                     /locus_tag="PLQ54_02110"
     CDS             373..1497
                     /locus_tag="PLQ54_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS92081.1"
                     /translation="MAGEKTRRDFLRDTALMTAGAFLAGSAARNAACAADQDIDPSLI
                     RNYNPGMRYRRLGKTGLWFSEISLGGHWRNRDGSRFWNEFSEEVVPDDVRSNRTEVVD
                     KCIELGINYVDLTTPAECLAYGAALKGRRDAMFVGADNHMLGPRNRENITVEKQIFNC
                     EECIRQLGFDYLDIWRIQADMGGRNSDDDVKICVEAFHKLHEQGKVQFLGFSCHNRDW
                     LKHLIETFPEVSMVVFPYTAKSATQEADPGHGADQKQSLFPALADRDVGSVCIKPFGG
                     GSLFRKQNIVFGQDMEGNAEDHEIAQLTLAYIVSNPYITCSVPGMTSVAEVVNNAAAS
                     KRAQELLKGEASLDRLNDHADRMMASLPHDYQWLHDWVRV"
     gene            1621..2100
                     /locus_tag="PLQ54_02115"
     CDS             1621..2100
                     /locus_tag="PLQ54_02115"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOS92082.1"
                     /translation="MTCNKAHLAALAAVLGLVALAAWPQDGGSTMDPAVPAGTPPNIA
                     LDVTDNWACLVCHLDFETETLTAQHADVGISCSRCHGPSDAHQHDEMSATTPDFLFGR
                     LEIEPLCKSCHPTGHKDQTKVDAFLAEWRGKQRPNGRTILDSSVCTDCHGEHALPKK"
     gene            2355..4019
                     /gene="ilvD"
                     /locus_tag="PLQ54_02120"
     CDS             2355..4019
                     /gene="ilvD"
                     /locus_tag="PLQ54_02120"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOS92083.1"
                     /translation="MKSDAMKSGDEGMPRRALIHATGLTHDEIRKPFVGIASSFSDLV
                     PGHTGMRDLERFVERGIHSAGGVGFVFGVPGVCDGVAMGHVGMQYSLPSREIIADIIE
                     CVAQAHALDGLVLLTNCDKITPGMIMGAARLDIPAVVVTAGPMLSGRYGGRKLDLVRD
                     TFEALGRLRAGTMDRHEFECLEVEACPGAGSCQGLFTANTMACLTEAMGLSLPYCGTA
                     LAVSAEKRRIAHDSGEAIVRLIREGITIRQILNSKAIDNALRIDMALGGSTNTALHIP
                     AIAIEAGLELPLERIDAISRETPHITHLRPGGDHMMEDLHWAGGIPAVQNVLGDRLAD
                     NPTVSGMSVLEIAARGRVTDANVIHSVANAYHAEGGIAVLRGSLCPDGAIVKQSAVPE
                     AQRVFEGPAVVFDGEQASLEALAEKRIQAGDVVIVRYEGPKGGPGMREMLRATAELAG
                     LGLMDKVALITDGRFSGGTRGLSIGHVSPEAAERGAPIGLVRDGDRIRIDLDKRRADL
                     LVDGAELAKRLEGWQPRTPAINHGLLARYARSVRSASFGATYAPPE"
     gene            4115..5794
                     /gene="ilvB"
                     /locus_tag="PLQ54_02125"
     CDS             4115..5794
                     /gene="ilvB"
                     /locus_tag="PLQ54_02125"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOS92084.1"
                     /translation="MITAAEALVQGLSRAGVDVIFGIPGGVLIPIYDKLLDACQSGAL
                     RNILVRHEQGAAHMADGYARASGRVGVCVATSGPGATNLVTGIATAYMDSVPIVALTG
                     QVRTSVIGTDAFQEADIFGITMPVVKHSYLVKRGQDLPQILCDAFYIASSGRPGPVLI
                     DIPADVSVAPFEPVVPEGPDTPGYRPTVRGNYRTIRRAAEVIAEAQRPLLYVGGGAVS
                     SGAADEVRKLAELTNIPVFTTLLGKGVFPETHRLSLGMAGMHGTAYANYAIQESDLLI
                     AIGARFDDRVTGDLKKFAPMARAIHIDVDPAEIGKNVSVTVPIVGDCKTVLAEMIPMV
                     KPREADHWNARCDDLKAQYPLRYKQSADTIMPQQAIEELHRLTSGEAIVVTGVGQHQM
                     WSAQHYLCSRPRQFLSSGGLGTMGYALPAALGAQVACPDQLVVAIDGDGSFQMTLQEL
                     ATGVAERLPVKVMILNNGYLGMVRQWQDLFYSKRYSGVDLAGSVDFVKLAEAYGAVGL
                     RATRPDELVPTLERAMAVTDRPCVIDVVVDREANVMPMIPSGRSVDDMRFE"
BASE COUNT         1010 a         1960 c         1887 g          952 t
ORIGIN      
        1 gctacagagg tcatgcgaca tacgcctctg gcattgggct tagggtcggc acccaggcac
       61 acccaaccgt aggcgaggct tcagcctcgc gcgaagtggc tctacaggcc attgtgagcc
      121 gaggctaaag ccccggctac agaggtgatg cgacatacac ctctggcatt gggcttaagt
      181 gcggaccatt cttacctgtc cccggtttcc gatggccctc tgccgcggca gagagctccg
      241 tcccctcgtc gtcagccatc cgccacgaga ggaatcacca gggcctgcgc gaagcacgcc
      301 ccgtgccacc aggctactgg ggcggcccca ctccgcgggc ggccccgttt cagcaagggg
      361 gattccacta ccatggcagg agagaagact cgaagggact tccttcggga cacggcgctt
      421 atgacagccg gcgcattcct ggcaggctcg gcggcacgca acgcggcctg cgctgccgac
      481 caggatatcg acccgtcgct gatccggaac tacaacccgg gcatgcgcta tcgccggctt
      541 gggaagaccg gattgtggtt ctccgagatc agcctgggcg ggcactggcg gaaccgcgac
      601 ggctcccgct tctggaacga gttctcggag gaggttgtcc ccgacgatgt ccggagcaac
      661 cgcaccgagg tcgtcgacaa gtgcattgag ctggggatca actacgtgga tctgacgact
      721 cccgctgagt gcctcgcgta cggcgccgca ctcaaaggcc gccgcgacgc catgttcgtg
      781 ggcgccgata accacatgct cggcccacgc aaccgcgaga acatcacggt ggagaagcag
      841 atcttcaact gcgaggaatg catccgccag ctcgggttcg attacctcga tatctggcgg
      901 atccaggcgg acatgggcgg caggaactcc gacgatgacg tgaagatctg cgtcgaggct
      961 ttccacaagc tacacgagca gggcaaggtt cagtttcttg gcttctcgtg tcacaaccgc
     1021 gactggctca agcatctcat cgagaccttt cccgaggtgt ccatggtggt cttcccatat
     1081 acggcgaaat ccgccacgca ggaggccgat cccggccacg gggccgatca gaagcagagc
     1141 ctgttcccgg ccctcgccga tcgcgatgtg ggctcggtct gcatcaagcc cttcggtggt
     1201 ggatcgctgt ttcggaagca gaacatcgtg ttcggccagg acatggaggg caatgcggag
     1261 gaccacgaga tcgcacagct caccctggcc tacatcgtgt cgaaccccta catcacatgc
     1321 agcgtgccgg gcatgaccag cgtggccgag gtcgtgaaca acgccgcggc ctcgaagcgc
     1381 gctcaagagc tcctcaaagg cgaggcctcg ctcgatcgac tcaacgacca cgccgaccgc
     1441 atgatggcca gcctaccgca tgactaccag tggctccacg actgggtccg cgtgtagtct
     1501 accccctgcc catcgggagc cgcccccttt ctacgggcgg agaggggtag gggatgaggt
     1561 gttatttggc ggggccggag cagccgtccg gccccgcttg gtgtgaggag gatccgctcc
     1621 atgacctgca acaaggccca tctggccgcc ctcgcagctg tcctcggcct cgtcgccctc
     1681 gccgcctggc cgcaagacgg cggatcaacc atggaccccg ccgtccccgc gggcacaccg
     1741 cccaacatcg cgctcgacgt caccgacaac tgggcctgcc tcgtctgcca tctcgacttt
     1801 gagaccgaga cactcaccgc ccagcacgcc gatgtgggca tctcctgctc gcgctgccac
     1861 ggcccctccg acgcccatca gcacgacgag atgtcggcca caacccccga cttcctcttc
     1921 ggccgcctgg agatcgagcc gctctgtaag agctgccacc cgacgggaca caaggatcag
     1981 acgaaggtcg acgccttcct ggcagagtgg cgcggcaagc agcgcccgaa cggccggacc
     2041 atcctggact cctcggtatg caccgactgc catggggagc acgcgttacc gaagaagtag
     2101 ggcgatgggc ccggcaccac ccggtggcag cggctggacg ctccgagagc cgccggcgga
     2161 tctgggttgc cgccactacc ctcggcccgc agagcacccg cccgttgccc tgccggccgt
     2221 tccttgacgc tcggacatcg cactgctatt ctccccactc aacccgacgg cccgcgcgcg
     2281 agtgccgtgc ttgtgcgcgc tggctgtgct tagacctacc cccttacttg tgctgccctt
     2341 cgggagatgc ggcgatgaag agcgatgcaa tgaagtccgg cgacgaggga atgccccgcc
     2401 gcgcactgat ccacgccacg ggtctcacgc acgatgagat tcgaaagccc ttcgtgggca
     2461 tcgcctcaag cttcagcgac ctggtgcccg gccacaccgg tatgcgcgat ctcgaacgct
     2521 ttgtcgaacg cgggattcac tccgcggggg gcgtgggctt cgtctttggg gttccgggcg
     2581 tctgcgatgg cgttgccatg ggacacgtgg gaatgcagta ttcgctcccc agccgagaga
     2641 tcatcgccga tatcatcgag tgtgtggctc aggcccatgc actagacggt ctggttctcc
     2701 tgacgaactg cgacaagatc acaccgggga tgatcatggg cgccgcccgc ctggacattc
     2761 ctgcggtcgt tgtcaccgcg gggccaatgc tgtccggacg gtacggtggc cggaagctcg
     2821 atctggtccg ggacacgttc gaggcgttgg ggcgactgcg cgccggcacg atggaccggc
     2881 acgagttcga gtgtctcgag gtcgaggcat gtcccggcgc tggctcctgc caggggttgt
     2941 tcaccgcgaa caccatggca tgcctgacgg aggcgatggg gctgtcgctg ccatactgcg
     3001 gcacagccct tgcggtatcg gcggagaagc gtcgcatcgc ccatgacagc ggcgaggcga
     3061 tcgtgcggct catccgcgag ggcatcacca tccgccagat actgaactcg aaggccatcg
     3121 ataacgccct acgcatcgac atggcccttg ggggatccac caacacggcg ctccacatcc
     3181 ctgccattgc catcgaggcg ggtctggagc tgccactcga gcggatcgat gccatcagcc
     3241 gcgagacccc gcacatcacc catctgcgtc cgggcggcga ccatatgatg gaggacctgc
     3301 actgggcggg aggcattccc gccgtgcaga acgtgctggg agaccgtttg gccgacaacc
     3361 cgactgtctc gggcatgtcg gtcctcgaga tcgccgcgcg cggccgagtc accgatgcca
     3421 acgtgatcca ctcggtggcc aatgcgtacc atgcggaggg cggcatcgcc gtgctccgtg
     3481 ggtcgctgtg ccccgatggc gccatcgtca agcagtccgc tgtgcccgaa gctcagcggg
     3541 tgttcgaggg ccctgccgtt gtgttcgatg gcgagcaggc gtcgctcgag gccctggccg
     3601 agaagcgcat ccaagccggg gacgtggtca tcgtccgcta tgaggggccc aagggtggcc
     3661 cgggcatgcg ggagatgctt cgcgcgacgg ccgagctggc cggcctcggg ctcatggaca
     3721 aggtggcgct catcaccgat ggacggttct cgggcggcac ccgggggcta tcgatcgggc
     3781 atgtgtcgcc cgaggcagcc gaacgagggg ctcccatcgg gctggtgaga gacggcgacc
     3841 ggatccgcat cgacctcgac aagcggcgcg ccgacctgct cgtggacggc gctgagctgg
     3901 ccaagcgact cgagggttgg cagccacgga caccggccat caaccacggc ctcctggcac
     3961 gctacgcacg cagcgtacga agcgcgagct tcggcgccac ctacgccccc cccgagtagg
     4021 gcggggcggc cggtgccagg gtaccattgc gccccgagcg gtccgcgcag cagttcccac
     4081 cgagcgccgg cagccatgga ggttcgatag ccctatgatc accgcagcgg aggccctcgt
     4141 gcagggcctg agccgcgctg gcgtggacgt catctttggc atccccggcg gcgtgctgat
     4201 ccccatctac gacaaactgc tcgacgcctg ccagagcggc gcgcttcgca acatcctggt
     4261 tcgccacgaa cagggcgccg cccacatggc ggacgggtac gcccgggcca gcggccgtgt
     4321 cggtgtgtgt gtcgccacct cggggcccgg ggccaccaat ctggtcaccg gtatcgccac
     4381 cgcgtacatg gactcggtgc ccatcgtggc cctcaccggc caggtgcgca cctcggtcat
     4441 cggcaccgac gcgttccagg aagcggacat cttcggcatt accatgcccg tggtgaagca
     4501 cagctacctg gtgaagcgtg gacaagacct gccccagatc ctgtgcgatg ccttctacat
     4561 cgcgtcatcg gggcggcccg ggcccgtgct gatcgacatt ccggcggacg tctccgtcgc
     4621 gcccttcgag ccggtcgtgc ccgaggggcc tgacacgccg gggtaccggc ccacggtgcg
     4681 tggcaactac cggaccattc gtcgcgcggc ggaggtcatc gccgaggccc agcgccccct
     4741 gctctatgtg ggcggcggcg ccgtcagctc gggtgcggcc gacgaggtgc ggaagctcgc
     4801 ggaactcacc aacatcccgg tgttcacgac cttgctcggc aagggcgtgt tccccgagac
     4861 gcatcgcctg tcgctgggta tggcgggcat gcacggcacg gcctacgcca actacgccat
     4921 tcaggagagc gacctgctga tcgccatcgg tgcacggttc gatgatcgtg tgaccggcga
     4981 cctgaagaag ttcgccccca tggcccgcgc cattcacatc gatgtcgacc cggccgagat
     5041 cggcaagaac gtctccgtga ccgtacccat cgtcggtgac tgcaagacgg tgctggccga
     5101 gatgatcccc atggtcaagc cccgcgaggc cgaccactgg aatgcgcggt gcgacgacct
     5161 gaaagcgcag tacccgctgc ggtacaagca gtccgcagat accatcatgc cccagcaggc
     5221 cattgaagag ctccatcggc tcacctcggg ggaagcgatc gtggtcacgg gcgtgggcca
     5281 gcaccagatg tggtcggccc agcactacct gtgctctcgc ccgcgccagt tcctcagttc
     5341 aggtgggttg ggaacgatgg ggtacgcgct gcctgcggcc ctgggggccc aggtggcttg
     5401 ccccgaccag ctcgtcgtgg ccatcgatgg cgatggcagc ttccagatga cgctccagga
     5461 actggccacg ggcgtggccg agcggctgcc cgtcaaggtc atgatcctga acaacgggta
     5521 cctcgggatg gtccgccagt ggcaggacct gttctacagc aagcgctact ccggggtgga
     5581 tctggcggga agcgtggact tcgtgaagct ggccgaggcc tatggcgccg tgggcctacg
     5641 cgccacgaga ccggacgagc tcgtaccgac gctcgaacgg gccatggcgg tcaccgaccg
     5701 cccctgcgtc atcgacgtgg tcgtggaccg cgaggccaat gtgatgccca tgatcccatc
     5761 gggcagatcc gtcgacgaca tgcgcttcga gtaggcgacg cgacgaaag
//
LOCUS       DAOSPL010000067         5802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_13955_length_5802_cov_3.254742, whole genome shotgun sequence.
ACCESSION   DAOSPL010000067 DAOSPL010000000
VERSION     DAOSPL010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13955_length_5802_cov_3.254742"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2026
                     /locus_tag="PLQ54_02130"
     CDS             <1..2026
                     /locus_tag="PLQ54_02130"
                     /inference="COORDINATES: protein motif:HMM:NF026221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOS92085.1"
                     /translation="SRANATDIRRRVPMHDPKWCIFFDFHTMPANPDVGKGFDFDAIT
                     GQLADAGVDYMVFPARCNLGMAYYNTKVGIRHPALEYDLIGRLSAACEKRGIALTTYI
                     NIGLSHEEALLHREWCVVTEEGYTYKPNRLDHFFRQMCYGTGYADHVIEMVREVASGY
                     PVAGFFFDCMYTSPCVGVECLREMRELGYDWQDAEQLHEYNYLKMNRMAQRLSDAARA
                     INPELLIYCNGIDYEAQRDSGTYLEFECLPTGGWGYEALPVGARYLRTLGKPVLNMSG
                     RFHKSWGDFGGIRTEPSLEYDLVYGLANAMRCTIGDHFHPRGDINQPVFDLYRHLYDR
                     LRPLEPWLEGARAEVDTAVVWPMPYPGYKCMSPAKRPEWDKHYTAIKGACRMLCELKW
                     QFDIVTDYIDWDAYDLLVLPDHTLLDEEWAERIRRHLAKGGAILSSAWSGLDPERKGF
                     VFPEWGARYGGDDPYDPAFFTPAKGMAAGLPDMPITLYQKGTSIEPLDGAEVLGEIIA
                     PYYNRHWDGEHGFLYMPPDKPAGRAFGVLRDRVAHFSHPLFTSYIVDGQIPVKRFVSN
                     TLARIMSEPLVRAPGMPSFARVNVTAQPHRRMLWVMSYVPERRGDTVDMIEEPIELRD
                     VAVSLRADGREVRSVYLAPDRQPLTFSLRDGYLHTVVPQVRGYAVVVFEE"
     gene            complement(2147..4459)
                     /locus_tag="PLQ54_02135"
     CDS             complement(2147..4459)
                     /locus_tag="PLQ54_02135"
                     /inference="COORDINATES: protein motif:HMM:NF027541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HOS92086.1"
                     /translation="MECIAVMATLALVQALSAVAADETIARYDLTPTLAADLSDPTRR
                     RAVWDEVHLVAALQGLANRHAPRLYLRYNAEADDFWWARMTEPGGWLEGRQARAVEGL
                     DALLERFADCYSGAVVWDERVPATSNLASTIAGCDSLLPIRFDETADSLYQRLVGAGR
                     LQPVVRLLADDSGPLFTGQGTVPGTAVASSGSAKCDAYLWLMEHYVKAGKTDPHRMGY
                     YLDGYWLTCWASGGPATHTLTNHDYVIAHRGVLFDLNVWDDETPIDDRGQPPGTDAAT
                     LRALLRCLYDQFNGKGTIQVAGFVPWAYKYTRFGSAGGTHAEVPTEWRYAEILSCFNA
                     YMDADALGLSAMANASFYQHYPLPERLPQRPKPTAESLRARGLLDADGRVVPRWYVAH
                     YVGDYDSAAWLYQCLPGMWADPARGKVPLSWAFNPNLAERFPLGMAWARQHATEADWF
                     VAGDSGAGYLNPGYLSEPRPHSGLPSGMAAWEEHCARFYRQWDLSVTGFVIDGFARGL
                     TTEGLDAYARFSPDGIVAQQIGAAGVHNGMPYLKMAGDLPGDPADAARQIVSRFHGPG
                     PQFAVFRSILQPPSWYLRVTERLDEVAQGNVVTVDLYTLLGLVRERESHPELYETPSA
                     YSSATAVAAAPGQELGLWPLWWDDGPFVVGDVDGVTCWRLPTHVPPYYLYFDVDDGFA
                     DSLRGTATIEVEYLDRGAGRFDLQYDSAESGAYCGSGAPVARQGTGAWRTAVFALDDV
                     RFAGAQNAGADFRLFSAGDDLVVRRVRVAR"
     gene            4667..5749
                     /locus_tag="PLQ54_02140"
     CDS             4667..5749
                     /locus_tag="PLQ54_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92087.1"
                     /translation="MAAHRRPRASTSLWLIAACLVAGAAAPSSALPVRVAGVVTDQAS
                     GEPVAGLAVRVGVFGAVRAIAPTDALGRFEAAFDSVATEFDVATDGNEIYLPAQVPHV
                     ASGQEVEVRLAPRAGTVQGTVCDAMTARPVAFVDVVASEGAEPLARARTDAQGVFSLD
                     VPLWRTGEPPARNALRTDLRLEVRATGYLPDRSPVLDLTPCVDGITPVRVALAVHPRV
                     GRIRVEVAGSGTRSPLCVPLIYGAGPRILGRTMTDGLGQATLELPAWTHEDRPADDNE
                     PRSDLWIAYSGIGYRSVKATGLSFSVGAGRLKPLRARIILAPSASTTASSSFAVVPEE
                     PPGAETDAPDHHPPQNGNRRPDWVPI"
BASE COUNT          935 a         1958 c         1972 g          937 t
ORIGIN      
        1 gtcccgtgcc aacgcgaccg acatccggag gcgggtcccc atgcacgatc ccaagtggtg
       61 catcttcttc gacttccaca ccatgcccgc gaaccccgac gtgggcaagg gcttcgactt
      121 cgacgccatc accggtcagc tggccgacgc cggcgttgac tacatggtct tccctgcccg
      181 gtgcaacctg ggcatggcct actacaacac caaggtcggc atccggcacc cggcgctcga
      241 gtacgacctg atcgggcggc tctcggcggc gtgcgagaag cggggcatcg cgctgacgac
      301 gtacatcaac atcggcctct cgcacgagga ggcgctgctc caccgcgaat ggtgtgtggt
      361 caccgaggag ggctacacgt acaagcccaa tcggctggat cacttcttcc gccagatgtg
      421 ctacggcacc gggtatgccg atcacgtcat cgagatggtg cgcgaggtgg cgtcgggcta
      481 cccggtggcc gggttctttt tcgactgcat gtacacgtcg ccgtgcgtgg gcgtcgagtg
      541 cctccgcgag atgagggagc tcggctacga ctggcaagac gccgagcagc tccatgagta
      601 caactacctc aagatgaacc gcatggccca gcggctctcc gatgcggcga gggccatcaa
      661 ccccgagctc ctgatctact gcaacggcat cgactacgag gcccagcgcg actcgggcac
      721 ctacctcgag ttcgagtgcc tgcccaccgg cgggtggggt tacgaggctc tgcccgtggg
      781 ggcccgatac ctgcgcaccc tgggcaagcc ggtgctgaac atgagtggcc gattccacaa
      841 gtcctggggc gacttcggag gcatccgtac cgagccaagc ctcgagtacg acttggtcta
      901 cggcctggcc aacgccatgc gctgcaccat cggagaccac tttcaccctc gaggcgatat
      961 caaccagccc gtgttcgatt tgtatagaca tctatacgac cgcctgcgtc cgctggagcc
     1021 gtggcttgag ggcgcgcggg ccgaagtcga tacggccgtg gtgtggccca tgccctaccc
     1081 cgggtacaag tgcatgagcc cggccaagcg gcccgagtgg gacaaacact acaccgccat
     1141 caagggcgcg tgccggatgc tgtgtgagct caagtggcag ttcgacatcg tcacggacta
     1201 catcgactgg gacgcctatg acttgctggt gctgcccgac cacacgctcc tcgacgagga
     1261 atgggccgag cggatccgca ggcatctcgc caagggcggc gccatcctct cgagcgcgtg
     1321 gtcggggctc gaccctgaga ggaaaggctt cgtgttcccc gagtggggcg cgaggtacgg
     1381 gggcgatgac ccgtacgacc cggcattctt cactcccgcg aagggcatgg ccgcgggtct
     1441 gcccgacatg ccgatcaccc tctaccagaa gggcacgagc atcgagccgc tggacggcgc
     1501 cgaggtgctc ggcgagatca tcgcgccgta ctacaaccga cactgggatg gcgagcacgg
     1561 cttcctgtat atgccgccgg acaagcccgc gggccgtgcc tttggggttt tgcgcgaccg
     1621 ggtggcccac ttcagccatc cgctgttcac cagctacatc gtcgatggac agatccccgt
     1681 gaagcggttc gtctccaaca cactggcgcg catcatgtcc gagccgctgg tccgggcccc
     1741 ggggatgccc tccttcgccc gagtgaatgt caccgcccaa ccccaccgga gaatgctatg
     1801 ggtcatgtca tacgttcccg agcgccgggg cgatacggtg gacatgatcg aggagcccat
     1861 cgagctccgt gatgtggccg tgtccctccg tgccgatggg cgggaagtga ggtcggtcta
     1921 cctcgcgccg gaccgccagc cactgacctt cagcttgcgc gatggctacc tgcacaccgt
     1981 cgtgccgcag gtgaggggct acgccgtcgt ggtgttcgag gagtaggggg caccccccta
     2041 tccggtacgc accggagaca tgggtaacgc gttggctcct ccgcaaggcc aggcaacaca
     2101 ctgggcgcgg cctcggcgcg gcgttcgcct ggcccagatc gctggcctat cgcgccactc
     2161 ggacccgccg caccaccagg tcgtcgccgg cgctgaacag ccggaagtcc gctccggcgt
     2221 tctgggcccc ggcgaaacgc acatcgtcaa gcgcgaacac cgccgtgcgc cacgcgccag
     2281 ttccctgtcg agcgaccggc gcccctgagc cgcagtaggc tccgctctcc gctgagtcgt
     2341 actgcaggtc gaagcggccg gcgcctcgat ccaggtactc gacctcgatg gtggccgtgc
     2401 cgcgcagcga gtccgcgaaa ccgtcgtcga catcgaagta gaggtagtac ggcggcacat
     2461 gagtcggcag gcgccagcac gtgactccgt ccacatcgcc gaccacgaac ggcccgtcgt
     2521 cccaccacag cggccacagg ccaagctcct gaccgggcgc cgcggcgacc gcagtggccg
     2581 aggagtaggc tgagggcgtc tcgtacagct cggggtggga ctcgcgctcg cggaccagcc
     2641 ccagcagcgt gtagagatcg acggtcacca cgttgccctg ggccacctcg tctagccgtt
     2701 cggtcacccg cagataccac gatggcggct gcaggatcga tcggaacacc gcaaactgcg
     2761 ggccggggcc gtggaagcgc gacacgatct gccgcgccgc gtcagccggg tcgcccggca
     2821 ggtcgccagc catcttcagg tagggcatac cgttgtggac gccggccgcg ccgatctgct
     2881 gagcgacgat gccgtcgggc gagaaccgtg cgtaagcgtc cagcccctcg gtggtcaggc
     2941 cccgagcgaa cccgtcgatg acgaacccgg tgacggagag atcccactgg cggtagaacc
     3001 gggcacagtg ctcctcccac gccgccatgc ccgacggcag gcccgagtgc ggccgtggct
     3061 ccgagaggta gcccgggttg agatagcccg ctccactgtc tcccgcgacg aaccagtctg
     3121 cctcggtcgc atgctgccgc gcccaggcca tgcccagcgg gaaacgttcg gccaggttcg
     3181 ggttgaaggc ccacgagagc ggcaccttcc ctcgggccgg gtcggcccac atgcccggca
     3241 ggcactgata gagccacgcc gccgagtcgt agtcgcctac gtagtgggcc acgtaccacc
     3301 gcgggacgac gcggccgtcg gcgtccagca gcccccgggc gcggagcgat tcagcggttg
     3361 gcttcggccg ctgcggaagc cgctcgggca gcgggtagtg ctggtagaac gaagcgttgg
     3421 ccatggccga gagacccagg gcgtcggcat ccatgtaggc gttgaagcag gagaggatct
     3481 cggcatagcg ccactcggtg ggcacctcgg catgagtacc gccggcgctg ccgaatcgcg
     3541 tgtatttgta agcccatggc acgaatccgg cgacttgtat cgttcccttt ccattgaact
     3601 gatcatataa gcatctaagc agagcacgca gagtagccgc atccgtgcct ggaggctgcc
     3661 ctcgatcgtc gatgggagtt tcatcatccc acacattgag atcgaacagc actccgcggt
     3721 gtgcgatcac atagtcatga ttcgtgagcg tgtgcgtcgc cggcccgccg ctggcccagc
     3781 aggtgagcca gtagccatca aggtagtagc ccatccggtg cggatccgtc ttccctgcct
     3841 tcacatagtg ttccatcagc cataggtagg catcgcactt ggcgctgccc gaggatgcca
     3901 cggcggttcc cggcacggtc ccctggccgg tgaagagcgg cccgctgtcg tcggccagca
     3961 gccgcacaac gggctgcagc cggccggcgc caacgaggcg ctggtagagg gagtcggccg
     4021 tctcgtcgaa gcggatgggc agcaggctgt cgcacccggc gatggtcgag gccaggttcg
     4081 aggtcgcggg cacccgctcg tcccagacca ctgcgcccga gtagcagtcg gcgaagcgct
     4141 ccaggagcgc atcgagcccc tcaacggccc tggcctggcg gccctccagc cacccgccgg
     4201 gctcggtcat ccgggcccac cagaagtcgt cggcctcggc gttgtagcgg aggtagagcc
     4261 gcggggcatg gcggttcgcc agcccctgga gtgccgcgac gaggtggacc tcgtcccata
     4321 ccgcccggcg gcgagtggga tcactcagat cggccgccag ggtgggcgtc aggtcgtacc
     4381 gagcgatggt ttcatcggcc gccacggccg acagtgcttg caccagagcc agagtggcca
     4441 tcaccgcgat gcactccatt cccgtgccga cgcctaggcg gggaggtcca caagaggcgc
     4501 cgccagcatt atgggcgcgg ctcacactcg cggtcgaggg gggcggcgag caacagggcc
     4561 tgggcgggcc gggttctgct cgtcggctaa ggttcgctgt ccggggtacc gatagtaccg
     4621 tgagaccttc tggctctcgg tgctctcggt tcatcggagg gggagtatgg ccgcgcaccg
     4681 ccgtcctcgc gcatctactt ccttatggct catagcggcg tgtctggtgg ctggtgcggc
     4741 ggccccctcc tccgcgcttc ccgtgcgtgt ggccggagtg gtcacggacc aggccagcgg
     4801 tgagcccgtg gctggtctgg ctgtgcgcgt cggagtgttt ggggcagtgc gggccatcgc
     4861 gcctacggat gccctcgggc ggtttgaggc cgcgttcgac tccgtggcca ccgagtttga
     4921 tgtcgccacg gacggcaacg agatctacct cccggcgcag gtgccgcacg tggcctccgg
     4981 tcaggaggtc gaggtgcggc tcgcgccgcg cgccggcacg gtgcagggca cggtgtgcga
     5041 tgccatgacg gcgcggcctg tggctttcgt cgatgtggtg gccagcgagg gggccgagcc
     5101 gctggcccgg gcccgcaccg acgcccaggg cgtcttctcg cttgatgtgc cgctgtggcg
     5161 gaccggtgaa ccgccggcgc gcaacgcgct gcggacggat ctccggctgg aggttcgggc
     5221 gacgggctac ctccctgatc gctcgccggt gctcgacctg actccctgtg tcgatggtat
     5281 cacgccggtg agggtggctc tggcggtgca cccgcgcgtg gggcggatcc gggtcgaggt
     5341 cgccggctcg ggcacgcggt cacccctgtg cgtacccctg atctacgggg ccgggcctcg
     5401 catccttggc cggaccatga ctgatggact cgggcaggcg acgctggagc ttccggcgtg
     5461 gacgcacgag gaccgtccgg ctgatgataa cgagccgcgg tcggacttgt ggatcgccta
     5521 ctcgggcatc ggctatcgga gcgtgaaggc cacggggcta tcgttctcgg tcggagccgg
     5581 gcggctaaag ccccttcggg cccgcatcat cctggcgcca tcagcctcga cgacggcgag
     5641 cagcagcttc gctgtcgtac ccgaggagcc cccgggcgcg gagaccgatg cgcccgacca
     5701 ccatcccccg cagaacggaa accgccggcc cgactgggtg ccgatctagg tccagcggct
     5761 cggcgatggc gtcgagcgcg acgtccagtg ggacttgggg cg
//
LOCUS       DAOSPL010000068         5784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_14014_length_5784_cov_2.885844, whole genome shotgun sequence.
ACCESSION   DAOSPL010000068 DAOSPL010000000
VERSION     DAOSPL010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14014_length_5784_cov_2.885844"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3952)
                     /locus_tag="PLQ54_02145"
     CDS             complement(<1..3952)
                     /locus_tag="PLQ54_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOS92088.1"
                     /translation="MSRWRLRGRYEVTHADVAWMVHLYLDGELGDQERQAFEAHIAAC
                     AECREVVDALAAEVPRVPRPVGPPATPPGFATRVACAVREEGLWRSSRAHAWIRASAW
                     VLALMGCALAVCTAYGARLPRLVGSGELTIAAAPEFAASSATTVRVALLDSRTRKPRV
                     GTKVWLAAYPPRSRSPYLTRVAYTDHEGTASVSIRIPDFDRVSVRLVARARNGWGDNQ
                     AQLWVPVHRRLRILVSSDQHVYRPGQEVRIRAQVLSSPGGEPAVGEPIALTIADPRGR
                     VLYRAADTVPGSGVITAKLPLAEVAEPGTYSISAAAAGTRGARTVVVESNEPSPAGIA
                     GVSPNPSAPESGPYIVAVPQGGALLAGTPAPVLVLTTDAAGRPVPCRLDTRFASGGHT
                     TRSTWYSGASGLTTASLMATGPSGSLSVVATDGRGRRASRRFDFPVRGRQGLVAFTDK
                     ALYRAGERLQFGVICGAEEEPQVLVDVLRESQPIWSGSARLSDGYAGVGIDLTGDLTG
                     LLVVQARRATMPDQAAAALAFVEPPQGLSVSLRPDRNAYSPGDTARLGVRVETPHGFP
                     AQASVWLAALDERSFRAATDSPAAFVARVSAQHDLLTPVGGSGAEALLCMLSTFVRDP
                     AHFGAQTNARCLLAACAPTPVLLAQGRDLPAAALARARTRGASTVATAMRLWLVWALG
                     SVAVAAAMHQAERRWRRRHTRFERHWAGPPRQAVALAALAVGLGALSIPWAVGLMRGA
                     PAPEPSSTAGSAPVQTASGTVRPSTVLGADDLDRLCRPYVPAIAGGQALAFSPDLDTD
                     ASGWTSAQVELPEAPGRWRQVALAVSPRGDIGWSEATIVSSSEVVVSLDVPPAAIQDD
                     CLVVPVTVRNEGTREALVDVSLAKADWFAPRSPLEARLAIPAGGASTGVFAVLARRAG
                     VGRLVVEARSDSTTTKAERFVDVAPSIPSRIALESGVAEGEAGLSVYIPDDAHLASAE
                     LMVTLFADLRSQALQALEMADQRPAPGCRDAAGWLQLAIAAQTLPRAASAEDEIPTPN
                     PNSRSVILALQTLLSYQASTGAFGPARVRSSATETSLALMALTELTRLRADAAPLRAT
                     TRAALARMQAPDGSFTGVGADPLARVRTTALAAWALAETGLADTARDRALAYVEGHID
                     TAHDGYTLALSALALQTSGSRSEASRRAAERLIGAAAPSGSGAPWPLEGPTLMGAEGD
                     LAAVEATALACAALADSGWPKTLMRRAVHWLYTHRGGDGMWGSPARAALVLGALSRLG
                     DELATARGLATVSVNDERLGQVRLNPGEAGSPQPIRGTGCLRVGENQVVIGVPRG"
     gene            complement(3949..4551)
                     /locus_tag="PLQ54_02150"
     CDS             complement(3949..4551)
                     /locus_tag="PLQ54_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS92089.1"
                     /translation="MLEVTSARVLTDELLVGRARSGHAADFDLLVQRYADRVYRFCLR
                     HLGDAEAAEDVAQTAFVEAFASLPRFDSRKPFGPWLFGIAANQCRLWHRRRRRSRWWS
                     IGSEDSEERLEAVPDEAHSPEEALEDEDRRRQVRRAVAGLPVRYREVILLRYLQGMST
                     REVAEALGLKPEAAAKRLTRGLQMLRDRLGELGFAGGDLA"
     gene            complement(4784..>5784)
                     /locus_tag="PLQ54_02155"
     CDS             complement(4784..>5784)
                     /locus_tag="PLQ54_02155"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOS92090.1"
                     /translation="DEYADFYLACREGVRSVDPNARVGAIAVRVVGNETPSQPGSWME
                     TVLRRCGASADFIDIHHGYAPLLRRSPAPGVPELTDDEFAEAFMGAPRYVDETIDAVE
                     RDIAAYVPGDGANLRVHITEYGPLVYPFGSGDPISDLRWNRSITGAVYLAGLYHVMAS
                     HPRIESANHLPLVQDIFGALVGIRSPGPRTWRNAVFYVFRTYSRLAGTTMVATHVTSP
                     AYSTRIAGFVPAMNDVPFVEAGAFRAADGRRLTVCLIHRSAVRPAEATLDPGRSATLE
                     RATVLAGDSCLAENTPERPDAVVPAAIAATQAEDRTVTLELPPHSLTVLEFELEGA"
BASE COUNT          956 a         2079 c         1953 g          796 t
ORIGIN      
        1 cgcctctcgg cacgccgatc accacctggt tctcgcccac ccgcaggcac ccggtgcccc
       61 gaatcggctg gggcgatccg gcctcgccgg ggttgagcct cacctgtccc agacgctcgt
      121 cgttgaccga gaccgttgcc aggccccgtg ccgtggcgag ttcatcgccg agtcgggaca
      181 gggcgcccag taccaacgcg gctcgtgccg gtgagcccca catgccatcg ccgccccggt
      241 gcgtgtagag ccagtgcacg gcacggcgca tgagggtctt cggccacccg gagtcggcca
      301 gcgcggcgca tgcgagagca gttgcttcca cggcagcgag atcgccttcg gctcccatga
      361 gggtggggcc ctccagcggc cacggggccc ctgagcccga aggcgccgcg gcgccaatga
      421 ggcgctcggc tgcccgccgc gacgcttcgg accgtgagcc cgacgtctga agcgccaggg
      481 cactcagggc cagtgtgtag ccgtcgtggg cggtatcgat gtgtccttca acgtaggcca
      541 gcgcccggtc gcgcgccgtg tctgccagac cggtctcggc cagtgcccag gcggccaggg
      601 ccgtggtgcg aactcgcgcg agcgggtctg cgcccacgcc ggtgaacgaa ccgtccggag
      661 cctgcatccg agccaacgcc gcccgggtgg tggcccggag cggagcggcg tcggcccgca
      721 ggcgagtgag ctcggtgagt gccatcagcg ccagcgatgt ctcggtggcc gaagagcgga
      781 cgcgcgccgg gccaaacgcg ccggtgctgg cctgatatga cagcagcgtc tgaagtgcca
      841 aaatcaccga acggctgttg ggattcggcg tcgggatctc gtcctcagcc gaggccgccc
      901 gggggagtgt ctgtgcagca atggcgagtt gcagccagcc agccgcatcg cgacaccccg
      961 gagctggtcg ttggtccgcc atctcgagtg cctgcagcgc ctggcttcgc aggtcggcaa
     1021 acagggtcac catcagctca gccgaggcca ggtgtgcgtc atcggggatg tataccgaga
     1081 gaccggcctc gccctccgcg acacccgatt cgagcgcgat ccgcgacggg atcgatggcg
     1141 ccacatcgac gaagcgttcg gccttcgtgg tggtcgagtc cgaccgcgcc tccaccacga
     1201 gacgcccgac cccagctcgg cgggccagca ccgcgaacac acccgtcgac gctccaccag
     1261 ccgggatggc gagtcgcgcc tccagaggcg accgtggagc gaaccagtcg gccttggcca
     1321 gcgaaacgtc caccaatgcc tcgcgcgtcc cctcgtttcg taccgtcacc ggcaccacga
     1381 ggcagtcatc ctggatggct gcgggaggca cgtcaaggga cacaaccact tccgaggagg
     1441 acacaatggt cgcctcgctc cacccaatgt cgccgcgagg cgacacggcc aaggcaacct
     1501 ggcgccaccg gccgggggcc tccggcaact ccacctgagc cgacgtccat ccgctggcgt
     1561 ccgtgtccag gtctggactg aacgcgagag cctggccccc ggcgatggcg ggaacgtacg
     1621 gcctacacag ccggtccaag tcgtcagcgc caaggaccgt cgacggtctc actgtgcctg
     1681 aggccgtctg aaccggggcg ctgcctgcag ttgaggatgg ctccggggcc ggtgcacccc
     1741 gcatcagccc gacagcccag gggatggaca acgcacccaa tcccaccgca agcgcggcca
     1801 aggccaccgc ttggcgaggg ggaccggccc agtggcgctc gaagcgcgta tggcgacgtc
     1861 gccaacgccg ctccgcctga tgcatagcag cggctaccgc cacactgccg agcgcccaga
     1921 cgagccatag gcgcatggcc gtggccaccg tgctcgcccc gcgcgttcgg gcccgggcca
     1981 gcgctgcggc cggcaggtcc ctcccctgcg ccaggagcac cggtgtcggc gcgcacgcgg
     2041 cgagcagaca gcgagcgttc gtttgggcgc cgaagtgggc cggatcacgc acgaaagtgg
     2101 acagcatgca cagcagcgcc tcggccccgg atccccctac aggcgtcaga aggtcgtgtt
     2161 gtgcgctgac tcgcgcaaca aaggctgcgg gactgtctgt cgccgctctg aacgagcgct
     2221 catcgagagc cgccagccac accgacgctt gagccggaaa cccgtggggt gtctcgactc
     2281 ggacgccgag ccgcgcggta tcgccgggtg agtaggcgtt tcgatccggc cggaggctga
     2341 ccgagaggcc ctgggggggc tccacaaagg ccagcgcggc agccgcctgg tcgggcatcg
     2401 tggcgcgtcg cgcctgcacc accagcaacc cagtcaggtc tcccgtgaga tcgatcccga
     2461 ctccggcgta cccgtcggac aagcgggccg aacccgacca gatgggctgg ctctcgcgaa
     2521 ggacatcgac aagaacctgt ggttcctctt cggcgccgca tatgacgccg aactgaagac
     2581 gctcgccggc gcggtacagc gccttgtcgg tgaaggccac caagccttgg cggccgcgga
     2641 ccgggaagtc gaagcgtcgg gaggcccttc gaccccgccc gtcggtggcc accaccgaga
     2701 gggagccact cggcccagta gccatgagcg atgccgtcgt cagtccggaa gcgccggagt
     2761 accacgtgga acgcgtcgtg tggccgccgc tggcgaatcg tgtgtcgagg cgacacggca
     2821 ccggccgccc ggcggcgtcc gtggtcaaca caagcaccgg agccggcgta ccggcaagca
     2881 gcgctccgcc ctgaggcacc gcgacaatat agggtccgct ctccggcgcc gaaggattcg
     2941 gagagacccc ggcaatgccc gccggagagg gctcgttgga ctccacgacc accgttcgcg
     3001 cgccccgcgt tcccgccgcc gcggccgaga tcgagtaggt tcccggctcc gccacctcgg
     3061 ccagaggcag cttcgcggtg atgacgcccg accctggcac ggtgtccgct gcccggtaga
     3121 ggacgcgtcc tcggggatca gcgatggtca gggcgatcgg ctcgccgacc gccggctccc
     3181 cgccgggaga cgagagcacc tgggctcgga tccgcacctc ctgccccgga cggtacacat
     3241 gctgatcgct agacaccaag attcgaaggc gccggtggac gggcacccac agctgagcct
     3301 ggttgtcgcc ccaaccgttc cgggcgcgcg cgacgagtcg gacactcaca cggtcgaaat
     3361 ccgggattcg gatcgagaca ctggccgtgc cctcgtggtc cgtgtatgcg acgcgagtca
     3421 gatacggcga tcggctacgc ggcggatagg ctgcaagcca caccttggtg ccaactcggg
     3481 gcttgcgcgt acgggaatcc aacaaagcga cccgaaccgt ggtcgcgctg ctggccgcaa
     3541 actcgggcgc ggcggcgatg gtcaactccc ccgagcccac aagccggggc aagcgggcgc
     3601 cgtaggcagt gcacaccgcc agcgcgcagc ccatgagagc caggacccag gcgctggcac
     3661 gaatccaggc atgggcccgg ctggatcgcc agaggccttc ctcgcggacc gcgcacgcca
     3721 cacgtgtcgc gaatccggga ggcgtcgcgg gcggcccaac cggccgaggc acgcggggca
     3781 cctcagcggc gagcgcgtcg accacctcgc ggcactcggc gcacgccgcg atgtgcgcct
     3841 cgaacgcctg ccgctcctgg tcgcccaact caccgtccag gtacagatgg accatccacg
     3901 cgacgtcagc gtgggtcacc tcataccgtc cgcggaggcg ccaccggctc atgccagatc
     3961 gccccccgcg aaccccagtt cacccagccg gtcacggagc atctgcaagc ctcgcgtcag
     4021 ccgcttcgcc gccgcctcgg gcttcaggcc cagagcctcg gctacctcgc gcgtggacat
     4081 cccttgcagg tagcgcagga gtatgacctc gcggtaccgt accggcaggc cggcgaccgc
     4141 acggcggacc tggcgtctgc ggtcctcgtc ctcgagcgcc tcctcgggcg agtgcgcctc
     4201 atccggaacg gcctccaggc gttcctcgct gtcctcggag ccgatgctcc accatcggga
     4261 gcggcggcgg cggcgatgcc acagccggca ctggttggcc gcgatgccaa acagccaagg
     4321 cccaaagggc ttgcgcgagt cgaaccgtgg aaggctggcg aacgcctcga cgaaggccgt
     4381 ctgcgcaacg tcctccgcgg cctccgcatc gccgaggtgg cggaggcaga agcgatagac
     4441 gcgatcggca tagcgctgga cgagcagatc gaagtcggcc gcatgacccg accgcgcccg
     4501 ccccaccagt aactcgtctg tcagaacccg cgcgctcgtg acttcaagca cggcctcggc
     4561 cccttggccc caggatccaa caggaatgcc ccaccggccg gccagaccgg tgggactccc
     4621 gatcctcgat tctcggccag atgcccgagt cagcgcaagc acagagggcc tccgaagcac
     4681 ggacgcgaag cgccgaagcc tgcgggcgct ccgtaccatg cctttgccct tgcgctctct
     4741 ctcgggacat ccgttggatg tccggggatc ggcgcgtgtt gcgtcaagcc ccttcgagct
     4801 cgaactcgag caccgtcagc gagtgtggcg ggagctccag ggtcaccgtg cggtcctccg
     4861 cttgggtcgc agcgatcgcc gccggcacca ccgcatcggg acgctccggt gtgttctcgg
     4921 caagacacga gtccccggcc agcaccgtcg cgcgctccaa cgttgcgctc ctgccaggat
     4981 cgagggttgc ctccgcaggc ctcacagcgc tccggtggat cagacagacc gtgaggcggc
     5041 gtccgtccgc cgcgcgaaac gcgcccgcct ccacaaaggg cacgtcgttc attgccggta
     5101 cgaagcccgc gatgcgcgtc gaataggccg gggacgtcac atgggtggcc accatcgtgg
     5161 tgcccgccag ccgcgagtac gtgcggaaca cgtagaacac cgcgttcctc caggtccgag
     5221 gcccggggga ccgaatcccg accagcgctc cgaagatgtc ctgcacgagc ggcaagtggt
     5281 tggcgctctc aatccgcggg tgggatgcca tgacgtggta gaggccggcc aggtataccg
     5341 cgcccgtgat actgcggttc cagcggaggt cggatatcgg atcgccgctg ccaaacgggt
     5401 agaccagcgg tccgtactcg gtgatgtgga ccctcaggtt ggcgccgtca ccgggcacgt
     5461 aggccgcaat gtcgcgctca acggcgtcga tcgtctcgtc cacataccgg ggcgcgccca
     5521 tgaaggcctc ggcgaactcg tcatcggtta gctctggtac tccgggcgcc gggctgcgcc
     5581 gcaggagcgg agcatagcca tggtggatgt cgatgaagtc cgccgaggca ccgcagcgcc
     5641 ggagcacggt ctccatccag gagcccggct ggctcggcgt ctcgttcccg acgacacgca
     5701 cggcgatggc cccgaccctt gcgttcgggt cgacgcttcg gacgccctca cggcacgcga
     5761 ggtagaagtc ggcatactcg tcgg
//
LOCUS       DAOSPL010000069         5668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_14411_length_5668_cov_3.564404, whole genome shotgun sequence.
ACCESSION   DAOSPL010000069 DAOSPL010000000
VERSION     DAOSPL010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14411_length_5668_cov_3.564404"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="PLQ54_02160"
     CDS             <1..141
                     /locus_tag="PLQ54_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HOS92091.1"
                     /translation="FRKYGESIGAATRDIAAGQHVHSHNMRSLRGQTDLPDQRLRGGS
                     PR"
     gene            138..1481
                     /locus_tag="PLQ54_02165"
     CDS             138..1481
                     /locus_tag="PLQ54_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS92092.1"
                     /translation="MMRVHRVVTATVLAVIGLGVTRAGAIEWIDGSIADGLRAAQLQK
                     RPLLVWVHYNSPDPTVAAKTAAQKTSAAVEAAFFNSAAYAPFEPQFAAMRVNFVVNET
                     VFDRYGVDRVVPAMLVLDQDTGKLAARIAGFPDQPEILASFCAERLTKASEVLRLSKV
                     PGGPQTAADRLRLGDLYADLGTLEQAGAAYEAALEAGLMDDDAHRAGVVAAFARLMSK
                     QPEDAMRLLNGALRGVRVRPWDEQCAPLWWPGEAAFAPLVLEDTRGKWLGALYRTRSL
                     RDFVAAVSGAERIAAQTGAAAQLSGPEALALGNDHFVLEDAAGAIPCYERAVTAGGLS
                     AADEEIARARVGLCRAQAGDKAGAMRDMDQFLAMYNRPKARLGLDEAHHRPQIMVTRA
                     GLYFDAGNYRAAEALLDQVVKDYTDTDCEYWAAQARVLLTHVKQWQELQRRGGAR"
     gene            complement(1522..1746)
                     /locus_tag="PLQ54_02170"
     CDS             complement(1522..1746)
                     /locus_tag="PLQ54_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92093.1"
                     /translation="MDDSGQVTSLTVREDRFHEIAEVVLRAMKDVTLGVHTVVADSVV
                     NAVKEAYLTGYHDGYVEGLRKGARGLPPDA"
     gene            2017..>2396
                     /locus_tag="PLQ54_02175"
     CDS             2017..>2396
                     /locus_tag="PLQ54_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92094.1"
                     /translation="MTFAWCVVAVAFQSCMQSGADGPLGVDPGTQPGSGDWSSERSLA
                     AKRESWSRLSALLDRVDQGGLAGLQAEELRELGRLHRRVASHLAQARSGRRDAALVAY
                     LNALVLRSHSTVYRTPSRPAGRALL"
     assembly_gap    2397..2406
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2407..3086
                     /locus_tag="PLQ54_02180"
     CDS             <2407..3086
                     /locus_tag="PLQ54_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOS92095.1"
                     /translation="QRGSTYVLAAVGLALLFTSVSFWASYCDPAMAAAFVPVGIYSKV
                     DPDSFDRTPELGTSADWVRSAFSSAIMWNNIRVGVLCFAGGILAGVPTLLMLAQNCFM
                     LGGLAGWCHASGVTWKFWALILPHGVVELTAIFVCAAAGFRLGYAVIAPGRYYRRDAL
                     ALAGRDAVRLALGTVPMFILAGLVEGFFSPTSLPDTVKLSFAAVLGSLTVVYLCFPRH
                     IEEPDDA"
     gene            3584..4042
                     /locus_tag="PLQ54_02185"
     CDS             3584..4042
                     /locus_tag="PLQ54_02185"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOS92096.1"
                     /translation="MAAWDDRRAEVDGQPAAAVECDPQLRDTSVLAVIDNGDPAAVLH
                     AALIACEAEAAQEGERLHLAPASERGAQPGLVAQMRARARARYLAEVLLPALELRGRD
                     PNAADRHGFTALHHAALNGQPEAVGVLLEAGSEVDAVTRGGQTPLLLASA"
     gene            complement(4263..>5668)
                     /locus_tag="PLQ54_02190"
     CDS             complement(4263..>5668)
                     /locus_tag="PLQ54_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92097.1"
                     /translation="RGRVESAGEAGWAPSGPSFRAHTMLTEASIRAAAVIRWVVLLPA
                     LAVACRAQTPVPAAVCTGRVVEFRVEGEALVSTPVGGVTIVAGPGLWLDVRPDGRDIV
                     SGGEPAHLGVTDADGVFSLPHDLAPDSVAILWKPGYLPAMVLPSEWADGPLEVVLIRQ
                     PGGGGHTALARSDGRLLFGAVTAPSGETVVDSEHGVALQAPPGYMRVHVDDDYTFLRL
                     AKGAREISVVDLKQKFDTRTLGQMLRSHTDGGEVKLLSDRRIDVDGVSATRREFRSPV
                     GRTVLVYASRGGSYYTFILYAPEKEYDEAAAEFEAALASLCFVGRSPSAPKGFRAAKL
                     PVWGLTYTVPPGWTAAPTREGAAVAEWSHSRLDARLVITGERSPMDSSPAATIERALA
                     ARGLAGLTLAASRPLLAGGLVGVDSTYTAEDGRMLRAAALEADGVSYCALFDLPPEPD
                     VATRQAVTACLRSMRVP"
BASE COUNT          846 a         1963 c         1949 g          900 t
ORIGIN      
        1 ttccggaagt acggcgagag catcggcgcc gccacgcgtg acattgctgc cggtcagcat
       61 gtccatagtc acaacatgcg atccttgcgg ggccagaccg atctgcccga tcagcgacta
      121 cgcggaggga gtcccagatg atgcgcgtcc accgggtcgt gacagccacg gtcttggcgg
      181 tgataggcct cggcgttacg cgggccggag ccatcgagtg gattgacggc tcgatcgcgg
      241 acggtctccg cgcggcccag cttcagaagc ggccactgct cgtctgggtg cactacaaca
      301 gccccgatcc caccgtggcc gcgaagaccg cggcccaaaa gacgagcgct gccgtcgagg
      361 ccgcgttctt caactccgcc gcctatgcac cattcgagcc tcagttcgct gccatgcggg
      421 tgaacttcgt tgttaacgag acggtattcg accgctatgg ggtcgatcgc gttgttccgg
      481 ctatgctcgt gcttgaccag gatacgggga agctcgcggc ccggatagcg ggcttcccgg
      541 atcagcctga gatcctggcc agcttctgtg ccgaacggct cacgaaagcc tccgaagtcc
      601 tgcggctcag caaggtgccc ggcggcccac agacggcggc cgaccgcctg cggctaggcg
      661 acctctatgc cgacttgggg acactggagc aggccggagc ggcctatgag gcggctctcg
      721 aggccgggct catggacgac gacgcgcacc gcgctggtgt tgtggctgcc ttcgcccggc
      781 tgatgtccaa gcaacccgag gacgcgatgc gtctgctcaa cggtgctctc cgcggagtaa
      841 gggtccggcc gtgggatgag cagtgcgcgc ccctgtggtg gccgggcgag gccgcatttg
      901 ccccgctggt gctcgaggat acccgcggca aatggctcgg agcgctatac cggacccgga
      961 gcctccgcga cttcgtcgct gcagtgtcgg gtgccgagcg gatcgcggcc cagaccggtg
     1021 cggccgcgca gctcagtggt cccgaggcgc tcgcgttggg caatgaccat ttcgtcctcg
     1081 aggatgcggc gggcgcgata ccgtgctatg agcgcgccgt gacggcaggg gggttgtcgg
     1141 ccgccgatga ggagatcgct cgggcccgcg tggggctctg ccgggctcag gcgggcgaca
     1201 aggcaggggc catgcgcgat atggaccagt tcctggcgat gtacaaccgg cccaaggcga
     1261 ggctggggct cgacgaagca caccatcggc cgcagatcat ggtcactcgc gcggggctct
     1321 acttcgatgc ggggaactac cgtgccgcgg aggcattgtt ggaccaggtc gtgaaggact
     1381 acaccgatac cgattgcgag tactgggcgg cccaggctcg ggtactactc acacacgtga
     1441 agcagtggca ggagcttcag cggcgtggcg gagctcgata gtcagcagtt agtccgccac
     1501 gcggcggggg agcctgaggg atcacgcgtc cgggggcagt ccgcgcgcgc ccttgcgtag
     1561 gccctcgaca tagccgtcat gatagccggt gaggtaggcc tccttgaccg cattgaccac
     1621 ggaatcggcc acaacggtgt gaacgcccag cgtcacatcc ttcattgctc gcaagaccac
     1681 ttcagcgatc tcatgaaaac ggtcctcgcg cacggtcagg ctggtgactt gcccgctgtc
     1741 gtccatccgt ccacctcgag atgcggtgcg tggggcggtc cggccctggg gaaccgccag
     1801 tggtagaata gtctatcctg tcggcacggg tccgtcacgg gcgggcggta accccgaccg
     1861 ggtcatcccg ttcccgggtg cgggcgcatg gagggagcca gccgcggcgg ctggagctct
     1921 atccggcccc aggccacttg tgcctgaggg ggcgcccggc ctggcggagg agcgagaggg
     1981 ccctggtaac gcccatcggg ggcaccggcc agtccgttga ccttcgcgtg gtgcgtcgtc
     2041 gctgtggcat tccagagctg tatgcagtcg ggagcggatg gcccattggg tgtcgatccg
     2101 ggaacccagc cggggtcggg ggactggagc tccgaacgga gtctcgccgc caagcgtgag
     2161 tcgtggtccc gcctctcagc ccttctcgac cgcgtcgacc aaggtggtct cgccggactt
     2221 caggccgagg agttgcgcga actgggacgc ctgcaccggc gggtggcctc gcatctggca
     2281 caagcgcgct ccggacggcg tgatgcggcg ctggtggcct atctcaacgc attggtgctg
     2341 cgctcccatt ccaccgtgta ccgtacgccg tcacgtccgg ccggccgcgc cctactnnnn
     2401 nnnnnngcca acgcggttcg acttacgtgc tcgccgccgt cggtctagcg ttgctcttca
     2461 cttcggtctc gttctgggcc agctactgcg acccggccat ggccgccgcg ttcgttccgg
     2521 tcggcatcta ctcgaaagtc gacccggaca gcttcgaccg gacacccgaa ctcggcacga
     2581 gcgccgactg ggtgcgctcg gccttcagct cggccatcat gtggaacaac attcgcgtgg
     2641 gcgtcctctg cttcgcagga ggcatcctcg cgggcgtacc cacgctcctc atgctggcgc
     2701 agaactgctt catgctcggc gggctggcgg gttggtgcca cgcctcgggc gtgacgtgga
     2761 agttctgggc gctgatcctc ccccacggtg tggtcgagct cacggccatc ttcgtgtgcg
     2821 ccgccgccgg cttccgtctt ggctacgccg tgatcgcccc gggccgctac taccgccgcg
     2881 acgccctcgc tctggcgggt cgcgatgcgg tccgcctggc cctggggacc gtgcccatgt
     2941 tcatcctcgc cggtcttgtc gagggtttct tctcgccgac gagcctgccc gacacggtga
     3001 agctgtcctt cgctgcggtg ctcggcagtc tgaccgtagt ctacctgtgc ttccccaggc
     3061 acatcgagga gccggacgac gcgtaggggc cggggctcgg gtaccggccc gaccgagggc
     3121 attccccttt gcctgctacc cactcgaggc tcgaaacggc ctgggtccgc tgcctcgacc
     3181 gaataatcca ggtcgtcgcc gaccatgcga ccggccggct ctgctctcgc gaagccgagc
     3241 tcgacttggg ccagcctcag cggttcgtcc ggctccgggc gcgggtactc gaggtgaagc
     3301 agggccacgc ggggaacact gggggtgcca ggggtgacta acccctcgga tgccgcgcgc
     3361 gggcgccggg cacggcgagc cggcgtcagc gtccggtgtg agggatgcga tgtcctcatg
     3421 atctccctgg tgagccgggc aaccgtggca cccgtgctcg tgctctgtac cctcgctgcg
     3481 gaactggtat gggcacggtc gctcacgctc ggcgatgtgc ttcacaccta cggcgcggtc
     3541 cccgggccgg gtggacccac cccctcccgg gtcggagttc gctgtggcgg cgtgggatga
     3601 ccgaagggcc gaggtagatg ggcagcccgc ggcagccgtg gagtgcgacc cgcagcttcg
     3661 ggacacgtca gtgctcgccg tgatcgacaa tggcgacccg gccgctgtgc tccacgccgc
     3721 gctgatcgcg tgtgaggccg aggcagccca ggagggcgag cgactccacc tcgccccggc
     3781 gtctgagcgc ggggcgcagc caggtctcgt cgcgcagatg cgcgccaggg ctcgagccag
     3841 gtatctcgcc gaggtactcc tcccggcgct ggagctgcgc ggccgggacc ccaatgcggc
     3901 ggaccgccac ggcttcacgg cgctacacca cgcggcactg aacggccaac cggaggccgt
     3961 gggagtgctc ctggaggccg gcagcgaggt ggacgcggtc actcgaggcg ggcagacgcc
     4021 cctgctgctg gcctcggcgt gagggacccg tcgctcgcgg cccgaggtcg gctgtcgccc
     4081 cgtggacggc ggcctgctgt cgggtacccg tcgccccctg catcccagct ccgcagtagc
     4141 ggggcacggc aacaccgtgg ggcgcccgcc ccacaccccg gccgactgaa gtcggcgctc
     4201 ccgaagatcc ccttgggcgc tggtcgattc ggggcaacac cgcagggcga aggtgctcgc
     4261 ccctacggca cccgcatcga ccgcaggcag gcggtgacag cctgccgggt cgcgacatcg
     4321 ggctccggcg gaagatcgaa cagggcgcag tagctcacac catcggcctc gagcgccgcg
     4381 gcgcggagca tgcggccgtc ctcggccgtg taggtgctgt ccaccccgac gagcccaccc
     4441 gccagcaggg gtcgcgacgc cgcaagagtc agaccggcaa gaccacgggc cgccagggcc
     4501 cgttcgatgg tcgcggccgg actgctgtcc atcggcgagc gttcgcccgt gatcaccagc
     4561 cgcgcgtcga gccgcgaatg gctccactcg gcgaccgcgg cgccctctct ggtcggcgcc
     4621 gctgtccacc cgggcgggac ggtgtaggtc agaccccaca cgggcagctt ggctgcccgg
     4681 aagcctttgg gcgccgatgg cgaccggccg acgaagcaca gactcgccag ggcggcctcg
     4741 aactcggccg cggcctcgtc gtactccttc tccggcgcat agaggatgaa tgtgtaatag
     4801 gacccgcccc gcgacgcata gaccagcacc gtgcgcccca cggggctgcg gaactcgcgg
     4861 cgcgtggccg atacaccatc gacatcgatc cgtcggtcgc tgagcagctt cacttcgccg
     4921 ccatcggtgt ggctgcgcag catctggccc agggtcctcg tgtcgaactt ctgcttgagg
     4981 tcgacgacgc tgatctcccg ggcccccttg gccaaccgaa ggaaagtgta gtcgtcatcg
     5041 acatggaccc gcatgtaccc cggtggcgcc tgcagggcca ccccatgctc cgaatcgacc
     5101 acggtctcgc ctgacggtgc ggtcaccgct ccaaacagca gcctgccgtc cgagcgggcc
     5161 agcgcggtgt ggccgccgcc ccccggttgc cggatgagga caacctcgag cgggccgtct
     5221 gcccactccg acggcaatac catcgccggc aagtacccgg gcttccagag gatcgccacc
     5281 gaatcggggg caaggtcgtg gggcagagag aagaccccgt ctgcatccgt cacaccgagg
     5341 tgagccggtt ccccgccact gacgatgtcc cgcccgtccg gccgcacatc gagccaaagc
     5401 ccggggcctg ctacaatggt gaccccgccc acgggtgtgc tgacgagtgc ctcaccctcg
     5461 acgcggaact caacgacgcg cccggtacag accgccgctg ggaccggcgt ctgggcccgg
     5521 cacgcgacgg ccagcgcggg gagcagcacg acccagcgga tcacggccgc tgcccggatg
     5581 gaggcctcgg tcaacatggt atgcgctcgg aaactcggcc cggagggcgc ccaccctgct
     5641 tcgccagcgc tctccactcg gcccctcc
//
LOCUS       DAOSPL010000070         5665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_14422_length_5665_cov_3.792692, whole genome shotgun sequence.
ACCESSION   DAOSPL010000070 DAOSPL010000000
VERSION     DAOSPL010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14422_length_5665_cov_3.792692"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /gene="mreC"
                     /locus_tag="PLQ54_02195"
     CDS             complement(<1..559)
                     /gene="mreC"
                     /locus_tag="PLQ54_02195"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOS92098.1"
                     /translation="MAQDWVGQEAAARSARRVSDAVTSVVSARRLSLQNDRLAAQTDL
                     LRRQNALLRQSDIAPVPLLGLEIQATRLRELTSTATRALVIGRGPDQWADALILNVGS
                     GSGVRAGDVVVSGPDLVGVIARVYWGTCVVRLVTDRYARLPARCARSQESLGMATGTS
                     RGLLQLRDLPLSSDLREGDLVCTAGL"
     gene            complement(671..1549)
                     /gene="mreC"
                     /locus_tag="PLQ54_02200"
     CDS             complement(671..1549)
                     /gene="mreC"
                     /locus_tag="PLQ54_02200"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOS92099.1"
                     /translation="MSSRIPLGIGAPSRPSRLRRGATALLLALAVTAVGLLVSRPSSP
                     MGAPAANLVTRQRSLIAQVAGLRAEQFLEQELLREGVRFRRLQELGAGLRRRGLSFVA
                     AQVVVNDPATSRVTLGRGSQSGVGHRDVVVAPEGLVGRVVTVNETTCTAALLTDPSVG
                     VPVVVAAHEPVGAANASPDTGGNGEGGLPRSQELHAIVTGSRPNGPLVLGYLSSGASL
                     RAGDPVLTSGIGEIYPPGLRVGVVLAEQARRPGVPEQALVRPLVEWQKLREVLIVRRP
                     VRGACGRGTVAHDLPA"
     gene            complement(1565..5578)
                     /locus_tag="PLQ54_02205"
     CDS             complement(1565..5578)
                     /locus_tag="PLQ54_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOS92100.1"
                     /translation="MMQELSELLKRNRGAMNRVVLFVGSGMALKPHRKTNEDIAVEAC
                     IRYAESQGIRLPEAMPERQEAAYDAVDAAHLSPAALSELVLPLVEDLQVGEGHLRLAR
                     LTKERYFSTIFTMCFDDLLERALAQEHLLPVEQYNLVVVGKATRREITGALMESQRLT
                     VVKLPGSAKDGPALTPRQVSSYLRRASHIVHELSKQCTLVVSYCARDRALMPIIAREG
                     DALWWINPQYPMSDRDAYDNLRIEQPDQLQHHAFQPEVVELLRSRRSERHVISREQGT
                     FDGFFAELYDRRVRRGSDRHLDVRRTDLKMEPEGPFKFLDHYEAKDASIFFGRDDDTR
                     RMRDLIDAHRVSVLFGRSGTGKTSLIHAGLVPPLEEEGALAITTRVLDEPATRITDEL
                     QKHPAVPEGLLGEPKIPLAEAIRAVREATGRQIVIFLDQFEEFFARLGERVRESFVQE
                     LAGCLACEDLDTRWVISIREDFFGELYELTESIPHILHAVYRLRRFAPSQARQAIVKP
                     AAKFELKFEEALIERILEDLHTDEGIGPAQLQIVCYRLVEQLGPRRRGVKLKDYETLG
                     QAEGILKDHLNYVIQQFAWRERDAARTVLKELVRSMQMKAPLPIKKICSDLNIDRDRV
                     ERILWQLTDYRLVRRLGDEHERQYELVHEYLADEIHGWMTDEELEAKDAQDLMARELN
                     DWREFEVLMRRESLKALHEHRMTLRTTPEELELILLSAAEQDFETDYWLPRSKELRHR
                     EVRFLRRILSSGRAKVRRYAVERLEALNTPEALDALLDALPQASADEAAVIAGALEKR
                     AGELVEAMSSSDTHIRTRAAYALGQIGNEVAIRGLVDVLARGEPLAALRSAAVEGLRT
                     TGFRAMARGQQAVTDALVASHRRRADDEANWLRAEALAGLVAGEASLKQLADAAETRG
                     RVPHLRYALGRVYYDQKRLAEAREQLDRLRADFPDVSDHEAVSRLLRAVGTLENELRE
                     GRYRWAMFRKGPDHAAWTPETLSTHLTLRWSVRTDGQVVSSAAVDAGLVYSGSRDGRF
                     RALHLRNGSVEWQVDVGARIESTPAVADGKVVFGANDGGLYCHEARTGMPVWTYRAGG
                     EIRASPTICDGRVYVGSWDRQLHSVDLETGERLWATPTGGEILASAAVADYVYAPSWD
                     GRLYALDPKTGEVVFAVSTEGELHSSPAYADGCVVFGSDAATVHCVDARTGEPRWVCD
                     AGGPVRSSPAIADRVAYVGGGDGVLRGLDLAGGSVVFEFRTGDGIQSSPAIADTILVV
                     GSRDGGLYLVDRRTGECLWSAMTSYAVTASPAIADGYVVSAIDYYYIAAFGPGNPEET
                     SGGSSLNLRQGAQRADG"
BASE COUNT          945 a         2060 c         1778 g          882 t
ORIGIN      
        1 ccaggcccgc ggtgcagaca aggtcgccct cgcgcaggtc cgaggagagt ggcagatcgc
       61 gcagttggag cagtcctcgc gaggtccccg tggccatccc cagactctcc tgggaccggg
      121 cacagcgggc gggcagccgc gcgtagcgat cggtgaccag ccgaacgacg caggttcccc
      181 agtacacgcg ggcgatgaca ccgaccagat ccggacccga gaccaccaca tcgccagcgc
      241 gcaccccgct gcccgagccc acgttgagga tcagcgcatc tgcccactgg tcagggccgc
      301 gcccgatcac cagggctcgc gtggcggtgg acgtgagttc ccgaaggcga gtcgcctgga
      361 tctcgaggcc gagaagcggc accggtgcga tgtctgactg ccgcaagagg gcgttctggc
      421 gacgcagcag gtccgtctgt gccgcgagac gatcgttctg gagcgatagc ctacgggcac
      481 tgactacgct cgtgaccgcg tcggagacgc ggcgcgcaga ccgcgctgcc gcctcttgtc
      541 cgacccagtc ctgggccacg gcaccggccc gctcccacac ggtcagcgcc ggtgagacgt
      601 cgccagtccg atggctcctg ccgacccaac cagcaacagc gcgagtatcg tccggcgccg
      661 tcgctcagga tcacgccgga agatcatgcg cgaccgttcc ccttccgcac gcgccacgca
      721 ctggacggcg aacgatcagc acttcgcgca gcttctgcca ctcgaccaga ggtcgaacca
      781 gggcctgctc gggcacccct ggccgccggg cctgctccgc gagcaccacg ccgacccgaa
      841 ggcccggcgg gtagatctcg ccgatccccg acgtcagcac gggatcgccg gcacgtaacg
      901 acgcgccact gctcaggtac ccgagcacca gcgggccgtt gggccgactg ccggttacaa
      961 tggcatggag ctcctgggag cggggtaagc ccccttctcc gttaccgccc gtgtcgggag
     1021 aggcattcgc ggcgccgacg ggctcgtggg cggcaaccac cacgggtacg cccacactcg
     1081 gatccgtcag cagggccgcg gtgcacgtgg tctcattcac agtcaccaca cggcccacca
     1141 ggccctcggg agccaccacc acgtcacgat gccccacgcc gctctgggat ccgcgaccca
     1201 gtgtcacacg agaagtcgcc ggatcgttca cgacaacctg tgccgccacg aaggacagcc
     1261 cgcggcgcct caagcccgcc cccagctcct ggagccgccg aaagcgcact ccctcgcgga
     1321 ggagttcctg ctcgaggaac tgctcggctc gaaggcctgc cacctgggca atcagcgagc
     1381 gctgccgcgt gaccaggtta gccgcagggg cacccatggg tgacgaaggc cgactcacca
     1441 gcagtcccac tgccgtgacc gccagagcca gcagcagagc ggtggcgccc cgccgcaggc
     1501 gcgacggacg ggacggggct ccgatcccca gaggtatccg gctactcatg gatctggccg
     1561 cagtctatcc atcggcgcgc tgggccccct gccggaggtt gagcgagctg ccgcccgaag
     1621 tctcctccgg gtttccgggc ccgaaggccg cgatatagta gtagtcaatg gcggacacca
     1681 cgtacccgtc cgcgatggcc ggggatgcgg tcaccgcgta gctcgtcatc gccgaccaga
     1741 ggcactcacc ggtccgccga tccaccaggt agagcccgcc gtcgcgagag cccaccacga
     1801 gaatcgtgtc ggcgatggcc ggcgacgact ggatcccgtc gcctgttcgg aactcgaata
     1861 ccacgctgcc cccggcaaga tcgagaccgc gcagcacgcc atcgccgccc cccacatacg
     1921 cgacacggtc ggcgatggcg ggagaggacc ggaccggtcc tccggcgtcg cacacccatc
     1981 gcggctcacc cgtccgggca tccacgcagt gcacggtggc cgcgtccgac ccgaagacca
     2041 cacagccatc ggcgtatgcc ggcgaggagt gcagctcacc ctctgtcgag accgcgaaga
     2101 ccacctcacc ggtcttggga tcgagcgcgt aaagccggcc gtcccacgag ggcgcataga
     2161 cataatcggc cacggcggca ctcgccagaa tctcaccacc cgttggagtc gcccagagac
     2221 gctcgcccgt ctccaggtcc acggagtgca gctggcggtc ccacgatccg acgtacaccc
     2281 ggccgtcgca gatggtcggc gacgcccgga tctcgccacc ggcccggtac gtccacaccg
     2341 gcatgccggt gcgcgcctcg tggcagtaca gcccgccatc gttggcgccg aagaccacct
     2401 tgccatcggc gaccgccggt gtggactcga tccgggcccc aacatccacc tgccactcca
     2461 cggaaccatt gcgcagatgc agcgcacgga accgtccatc cctggaccct gaatacacca
     2521 gtcccgcgtc gaccgcggcc gagctcacca cctgaccgtc agtgcgcacc gaccatcgca
     2581 aagtcaggtg ggtgctgagc gtctccggcg tccacgccgc atggtcgggg cccttgcgga
     2641 acatggccca gcggtaccgg ccctcccgca gctcattctc cagcgtgccg accgctcgca
     2701 acaggcggga gacggcctca tgatcactga cgtcggggaa gtccgcgcga aggcgatcca
     2761 gctgctcccg ggcttcggcc aggcgcttct ggtcgtagta cacgcgaccc agggcatagc
     2821 gcagatgcgg cacgcgcccc cgcgtctcgg cggcatcggc gagctgcttg agcgacgctt
     2881 ctcccgcaac cagccccgcg agggcctccg ctcggagcca gttggcctca tcatcggccc
     2941 gccgccggtg tgacgcgacc aaggcatcgg tcaccgcctg ttggcctcgg gccatggcgc
     3001 ggaaccccgt ggtgcggagc ccctccacgg ccgccgatcg cagagccgcc aacggctccc
     3061 cccgtgccag cacgtcgacc aggccgcgga tggcgacctc gttgccgatc tggccgaggg
     3121 cgtacgcggc gcgcgtgcgg atgtgggtgt cgctcgatga catggcctcc acgagctcgc
     3181 ccgcgcgctt ctccagggca cccgcgatca ccgcggcctc gtcggccgaa gcctggggca
     3241 acgcatccag gagcgcgtcg agtgcttcgg gtgtgttcag cgcctccagc cgctcgaccg
     3301 cgtaccgacg gaccttggct cgtcccgacg acaggatgcg acgtaggaat cggacctctc
     3361 ggtgacgcag ctccttggac cttgggagcc agtagtcggt ctcaaagtcc tgttccgccg
     3421 cgctgagcag gatcagctcg agctcctcgg gtgtggtgcg caacgtcatc cgatgctcgt
     3481 gcagtgcctt cagggactcg cggcgcatga gcacctcgaa ctcgcgccag tcgttgagct
     3541 ctcgcgccat caggtcctgg gcgtccttgg cctccagctc ttcgtcggtc atccacccat
     3601 ggatctcgtc ggccagatac tcatgcacca gctcatactg ccgctcgtgc tcgtcgccga
     3661 ggcggcgcac gagccggtag tccgtgagct gccacagaat ccgttccacg cggtcgcggt
     3721 cgatgttgag gtccgagcag atcttcttga tgggcagcgg ggccttcatc tgcatcgacc
     3781 gcacgagctc cttgagcaca gtgcgggccg cgtcccgctc gcgccaagcg aactgctgga
     3841 tgacatagtt gagatgatcc ttgaggatgc cctcagcctg acccagtgtc tcgtagtcct
     3901 tgagcttgac tccgcgccgc cgcgggccca gctgctcgac caaccggtag cacacgatct
     3961 ggagctgggc cgggccgatg ccctcgtcgg tgtgaaggtc ctcgaggatc cgctcgatca
     4021 gcgcttcctc gaacttcagc tcgaacttcg ccgccggctt cacaatcgct tgccgcgcct
     4081 gagacggggc gaagcggcgc aagcggtaca cggcatgcag gatatgcgga atgctctcag
     4141 tcagctcgta cagctcgccg aagaagtcct cgcggatgct gatcacccag cgcgtgtcca
     4201 gatcctcgca cgcgagacac ccggccagct cctggacgaa actctctcgc acccgctcgc
     4261 ccagtcgggc aaagaactcc tcgaactggt cgaggaagat cacgatctga cgtccggttg
     4321 cctcgcgaac cgcgcggatc gcctcggcca gcggtatctt cggctctccc aggagtccct
     4381 caggcaccgc gggatgtttc tgcagctcgt cggtgatgcg ggtcgccggt tcgtcgagca
     4441 cccgggtggt gatggcgagg gcgccctctt cctcgagtgg aggcaccaga cccgcgtgga
     4501 tcagcgaggt cttgcccgtc cctgagcggc cgaacagaac actcacacga tgagcatcga
     4561 tcaggtcgcg catccggcgg gtgtcgtcat cgcggccgaa gaagatgctc gcgtccttgg
     4621 cctcatagtg atcgaggaac ttgaagggtc cctcgggctc catcttcaaa tcggtccggc
     4681 gcacatccag gtggcggtcc gaaccccgcc gcacccgccg gtcgtacagc tcggcgaaga
     4741 acccgtcgaa tgtgccctgc tcccggctga tgacatgccg ctcgctccgg cggcttcgca
     4801 gcagctccac gacttcgggc tggaacgcat ggtgctgaag ctggtcgggc tgctcgatcc
     4861 gcaggttgtc gtaggcatca cggtcgctca tggggtactg cgggttgatc caccacagcg
     4921 cgtcgccctc gcgcgcaatg atcggcatca gggcacgatc ccgcgcacag tacgacacga
     4981 ccaatgtgca ttgcttcgaa agctcatgca cgatgtggct ggcccggcgc aggtacgagc
     5041 tcacctgccg gggcgtcagt gccggcccat ctttggcgct cccggggagt ttgaccacgg
     5101 ttagccgctg tgactccatc agcgcgcccg tgatctcccg gcgcgtcgcc ttcccgacca
     5161 ccaccaggtt gtactgctcc accggaagaa ggtgctcctg tgccagcgcg cgctcgagca
     5221 ggtcatcgaa gcacatggtg aagatggtgc tgaagtaacg ctccttggtc aggcgagcca
     5281 gacggaggtg cccctcgccg acctgcagat cctccaccag gggcaacaca agctccgaca
     5341 gagccgccgg cgagaggtgg gccgcgtcca cggcgtcgta ggccgcctcc tgcctctcgg
     5401 gcattgcctc cggaagcctg attccctggc tttcggcata ccgtatgcac gcctctaccg
     5461 cgatgtcctc gttcgtcttc cgatgcggct tgagcgccat gccgcttccc acgaacaaga
     5521 ccacgcggtt catcgcacca cggttgcgct tgagtagctc tgagagctcc tgcatcatcc
     5581 cgtgccgccc cagccggatc ttgcccggcc cgtgcggcca atccccctca tgctcgactc
     5641 cggcagcgag cgggtcccgc gcctc
//
LOCUS       DAOSPL010000071         5630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_14531_length_5630_cov_3.590852, whole genome shotgun sequence.
ACCESSION   DAOSPL010000071 DAOSPL010000000
VERSION     DAOSPL010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14531_length_5630_cov_3.590852"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="PLQ54_02210"
     CDS             <1..1231
                     /locus_tag="PLQ54_02210"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS92101.1"
                     /translation="QGMTAGAAALAFSPTRARAALGPNDRIRLALIGCGGMGNRHLDS
                     LVVRTDCEVVATCDVNRPRAEAARQKVGGACEAYQDFRRVLDRSDIDAVFCATPDHWH
                     ALVTIMACQAWKDVYVEKPLATTIAEGRAMVNAARRYGRVVQVGTQQRSQMLFRDAVG
                     IVHSGKLGTITGAGSWIGPNGATGIETPQPVPEGLDWDLWLGPAPAVPYSPQRYGAFR
                     AFHDYANGELTNWGVHLLDIVLWGIREDWPLSVQALGGSYRQIPGSDDYENVEVLYGF
                     RRCSVSWEQRHSTTIGGKGYGIRFTGTAGRLYMDRGSFVVEPPALGIAEEIEKGDPWI
                     DVQSHHTNFLECMRSRQRPNADVERGHRATSLVLLGAIALDCGRTLKWDGEAERFVGD
                     EAANRYLSRPYRAPWHL"
     gene            1274..3358
                     /locus_tag="PLQ54_02215"
     CDS             1274..3358
                     /locus_tag="PLQ54_02215"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOS92102.1"
                     /translation="MTRHFSILGSLVIIAAGAAAFGAADMMSYVEALQGDAAARVDAR
                     RMLPTYGVDAVAPVLPLLSHADGEISKAAFDILWEIANSAMSPGREAEAMRVAQALLG
                     ALATDAAEAIRLKLLRLLAIGAPEGCDLAPIALLLGDAALGEHARTTLERIATTEARG
                     ALRDALVGADGAWARALVNSLGALRDAESLPAIAGLLASNDAGLRAAAARALAWTGDP
                     AYADRLRRVAAEATPETQFDATDALLRYADAMAVRGGNWDRAMALFREVLESQPEGVL
                     KDGALVGLGRFGDETVVAPILATLASAEDPRTRGACMVALEALKGPGPGKALVAAYPG
                     LTGQAQVAMIAMFGRKGGAAVLPAILEAARSEEAELRQTALRALGICAEPGGVPVLAQ
                     AAVSGPDEERPLAVDALARAAQTLRERGRTDDAGRAYGALYEAAPTPELRQTALDGII
                     ACPVAEAFDIVFRVGGELEPSALSPPAQIAMLGALAKVGRKGEAMELLDALTNQPLVG
                     DGVAQLAASLAPLAGDMNVPQRLGFVTDWRVVGPFPFGGPGSGLDEEHVGEPKVDLAA
                     RYQVGDAEVAWQEVHSRDLAGIVSLHDVFGMVERRVCYAYTRIDVPEGGDAILRCGSD
                     DGIKAWVNGEVVLNHDVDRGAQVDQDQAPCTLRAGTNELLVKISQGGGGWNFCLRFTT
                     PDGRPLVSSPAR"
     gene            3403..4788
                     /locus_tag="PLQ54_02220"
     CDS             3403..4788
                     /locus_tag="PLQ54_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3696 domain-containing protein"
                     /protein_id="HOS92103.1"
                     /translation="MFRRLRIQNFKCWADTGEMRLAPITVLFGRNSSGKTSIIRFLLM
                     LKQTVESPDRLRVLHLGDSASAADLGTYHDFVHGHDARRDLRFELEWDLPSRLVLSEV
                     DETGSRTFAGRALRFEAAVGEANGAGALSVRRMEYVLGPDEADGTRVWMCAAEDRTRH
                     ELGSSPLAMRRRQGRPRQRVAPVKFYGFPEEEVAAHQDAGGLLDLVLALEQRLRGLHY
                     VGPLRGYPNRSYVWSGQAPQEVGARGADVVPALLGARDRAISPGPRRKSVPFHELIAR
                     WLRELGLVHSFEVKPLASHRREHEVLVRAWGGGPEVNLVDVGFGISQVLPVIVQCFYA
                     TRGSLVILEQPEIHLHPSAQSGLADLLTEAIHAREDGEDRGVQLLVESHSEHLLERLQ
                     RRIAEGKLSKDDVAVYFCEQEGAASKMTPLELDRFGNISNWPDGFFGDEMGDVLARAE
                     AEAKRRQGALK"
     gene            4785..5306
                     /locus_tag="PLQ54_02225"
     CDS             4785..5306
                     /locus_tag="PLQ54_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92104.1"
                     /translation="MSDFVIDTNVAVVADRRGPSNTTEGCILAAIAALRRVQKSDRLV
                     LDADGRILAEYTENVRTAGQPGPDQRFLRWVHRHQYNEQRCVRVAIHVRGDEGEDYEE
                     FPDAADLTDFDRSDRKFVAVALARGPVTPIIQATDAEWWRRRHALASAGVRIEFICPD
                     DIQRSHRALLNTR"
     gene            5405..>5630
                     /locus_tag="PLQ54_02230"
     CDS             5405..>5630
                     /locus_tag="PLQ54_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007695820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS92105.1"
                     /translation="MADTLRAGVIGCGVMGADHARLYAEMADVELVVVMDPAGARARE
                     LAGLHGCEVCTDLDTVAEFGLDVVSICVDDR"
BASE COUNT          889 a         1786 c         2051 g          904 t
ORIGIN      
        1 ccaaggaatg actgccggtg ccgcggccct ggcgttctcg cccacgcgag ctcgagcggc
       61 cctgggcccc aacgaccgca tccggctggc tctgatcggc tgtgggggga tggggaatcg
      121 gcacctcgac agcctggtcg tccgcaccga ctgcgaagtg gtcgcgacct gcgacgtcaa
      181 tcggccacga gccgaggccg cccgccagaa ggtgggcgga gcgtgcgagg cctatcagga
      241 tttccgccgc gtcctcgacc ggtccgacat cgacgccgtc ttctgcgcca cgccggatca
      301 ctggcacgcc ctggtcacga tcatggcctg tcaggcttgg aaggacgtct acgtcgagaa
      361 gccgctggcg acaaccatcg cggagggacg ggcgatggtg aacgcggcgc ggcggtatgg
      421 ccgcgtggtc caggtgggca cccagcagcg gtctcagatg ctgttccgcg atgcggtggg
      481 cattgttcac agcgggaagc tggggaccat caccggcgcc ggatcgtgga tcggcccaaa
      541 tggcgccacg ggcatcgaga cgccccagcc ggtccccgag gggctggact gggatctgtg
      601 gcttggcccc gcccccgcgg tcccgtacag cccgcagagg tacggcgcct tccgtgcctt
      661 ccacgattac gccaatgggg agctgaccaa ctggggcgtt catctgctgg atatcgtcct
      721 gtggggcatc cgcgaggact ggcccctctc cgtgcaggct ctaggcggca gctaccggca
      781 gatccccggc agcgacgact atgagaacgt cgaggtcctc tacggattcc gccggtgcag
      841 tgtgagctgg gagcaacggc atagcacgac catcggcggc aaggggtatg ggatccggtt
      901 caccggcaca gcggggcgcc tctacatgga ccggggcagc ttcgtggtgg agccaccggc
      961 cttggggatt gccgaggaga tcgagaaggg cgatccgtgg attgacgtgc agtcgcacca
     1021 cacgaacttc ctcgagtgca tgcgaagccg gcagcggccc aacgcggacg tggagcgggg
     1081 ccaccgggcg acatccctgg tgctgctggg agcgatcgcg ctggactgcg ggcgcacgct
     1141 gaagtgggat ggcgaagcgg agcgattcgt cggcgacgag gcagcgaacc ggtatctcag
     1201 ccggccctat cgcgctccgt ggcaccttta ggcccctctg tgcacgcacg gtgaagaacc
     1261 acggaggtct gacatgaccc gccacttctc gatcctcggc tcgctcgtca tcatcgccgc
     1321 tggggcagcg gccttcgggg ccgccgacat gatgagctac gtcgaagcgc tgcagggcga
     1381 cgccgccgca cgcgtcgacg ctcgacggat gctgcccacc tacggtgttg atgccgtggc
     1441 accggtgctt ccgctgctgt cccacgcgga tggcgagatc agtaaggccg ccttcgacat
     1501 cctctgggag atcgccaaca gcgccatgtc acccgggcgg gaggccgagg cgatgcgtgt
     1561 cgcgcaggca ctcctgggag cgctggcgac ggacgcggcg gaggcgatcc ggctcaagct
     1621 tctgcgcttg ctcgctatcg gcgcgcccga gggctgcgac ctggccccga tcgcgttgct
     1681 tcttggcgac gcggctctcg gcgagcatgc ccgaacgaca ctcgagcgga tcgcgacgac
     1741 cgaggcgcgc ggggcactcc gcgatgcgct ggtgggggct gacggcgcat gggccagggc
     1801 gctggtcaac tcgctgggcg cgctgcgcga cgccgagtcc ttgccggcca ttgccgggct
     1861 tctcgcgagc aacgacgccg gtcttcgggc tgcggcggcc cgggccctgg cctggaccgg
     1921 cgatccggcg tatgcggacc gcctccgccg ggtggctgcc gaggcgacgc ccgagaccca
     1981 gtttgatgcc acggacgcgc tgctgcgcta tgcggacgcc atggcggttc gcgggggcaa
     2041 ctgggaccgc gccatggcgc tgttccgcga ggtgctcgag agccagcccg agggagtgct
     2101 gaaggatggc gcactcgttg ggctgggcag gttcggcgat gagaccgtag tggctcccat
     2161 cctggccacg ctggcctcgg ccgaggatcc tcgaacgcgc ggcgcgtgca tggtggcgct
     2221 ggaggcactc aagggccccg ggccaggcaa ggcccttgtc gcagcctatc ccgggctgac
     2281 ggggcaagct caggtagcga tgatcgcgat gttcggacgc aagggcgggg cagccgtgtt
     2341 gccagccatc cttgaggcgg cgaggagtga agaggccgag ctgcgccaga cggcgctgcg
     2401 cgccctgggg atctgtgccg aaccgggcgg cgtgcccgtg ctggcccagg cggcagtctc
     2461 gggcccggat gaggagcgtc cgctcgcggt agacgcgctg gcgcgggccg cccagacgct
     2521 tcgcgagcgg ggccgcaccg acgatgcggg ccgcgcgtac ggggccctgt acgaggcggc
     2581 gccgacgccg gagctgcgtc agaccgcgct ggatggcatc atcgcgtgtc cggtagccga
     2641 ggcgttcgac attgtgttcc gcgtgggcgg cgagctggaa ccgtccgctc tgtcaccccc
     2701 ggctcagatc gcgatgctgg gcgcactggc caaagtgggg cgcaagggcg aggccatgga
     2761 gctcctcgac gcgctcacga accagccact cgtgggcgat ggtgtggccc agctcgccgc
     2821 gtcgctggct cccctggccg gggatatgaa tgtcccacag cggcttgggt tcgtgacgga
     2881 ctggcgtgtg gtcgggccct tccccttcgg cgggccgggg tcagggctcg atgaggaaca
     2941 cgtgggcgag ccgaaggtcg atctggccgc tcggtaccag gtcggcgacg cggaagtggc
     3001 ctggcaggaa gtgcacagcc gagacctggc gggcatcgtc agcctgcacg acgtgttcgg
     3061 tatggttgag cggcgcgtct gttacgccta cacgaggatc gatgtgcctg agggcggaga
     3121 cgcgatcctt cgttgtggca gtgacgacgg gatcaaggcc tgggtcaacg gtgaggtcgt
     3181 gctcaatcat gatgtggatc ggggggccca ggtcgatcaa gaccaggcgc cctgcacgct
     3241 tcgggcaggc acgaacgagc tgttggtgaa gatcagccag ggcggcggag ggtggaactt
     3301 ctgcctgcgg ttcaccacac ccgatggccg tccactggtg tcgagcccgg cgaggtagag
     3361 acagcgctcg agtggcaccg acagagggga ggagtcctgc cgatgttccg ccggctccga
     3421 atccagaact tcaagtgctg ggcagacacc ggcgagatgc gcctggcccc catcacagtg
     3481 ctgtttggcc ggaacagctc cggcaagacg agcatcattc gcttcctgct catgctgaag
     3541 cagacggtgg aatcgccaga caggctgcga gttcttcacc tcggcgactc ggcatcggcc
     3601 gccgaccttg gcacgtacca tgacttcgtc cacggccacg acgcgcgtcg agacttgcgg
     3661 ttcgagctcg agtgggacct gccctcgcgt ctggttctca gcgaggtcga tgagacaggc
     3721 agccggacct ttgccgggcg ggcgcttcgc ttcgaggccg ccgtcggcga agcgaacggg
     3781 gcgggcgcac tctcggtgcg tcgcatggag tacgttctcg ggccggatga ggccgatggc
     3841 acgcgagtgt ggatgtgcgc ggccgaggac cgcacccgcc acgagctggg cagttcgccc
     3901 ctagccatga gacgcaggca gggccggccg cggcagcgcg tcgctcccgt caagttctat
     3961 ggcttccccg aggaggaggt ggcggcccac caggatgccg gcggccttct cgatctggtt
     4021 ctcgcactcg agcagcggct aaggggcctc cactacgtgg gcccactcag ggggtaccca
     4081 aaccgctcct atgtgtggtc cgggcaggca ccccaagagg tgggggctcg gggggccgat
     4141 gttgtcccgg ccctactcgg cgctcgtgac cgggcgatct cgccgggccc acggagaaaa
     4201 tcggtaccat tccacgagct catcgcgcgc tggctccgcg agctcggact cgtacactcg
     4261 ttcgaggtga agccgctcgc gagccatcgc cgcgagcatg aggtcctcgt ccgcgcgtgg
     4321 ggcggcggcc ctgaggtgaa cctggttgac gtgggattcg gcatctccca ggtcctcccc
     4381 gtcatcgttc agtgcttcta cgcgacgcgg ggttcgctcg tcatcctgga gcaacctgag
     4441 atccacctcc acccttccgc ccagtctggg ctggcggacc tgctgacgga ggccatccat
     4501 gcgcgggaag atggcgagga ccgcggcgtg caactcctgg tcgagagcca ctcggaacac
     4561 ctcctggagc ggctacagcg gcggatcgcg gaggggaagc tgagcaagga tgatgtggcg
     4621 gtctacttct gcgagcagga aggggcggcc tcgaagatga cgccgctcga gctcgaccgg
     4681 ttcggcaaca tcagcaactg gcccgatggg ttcttcggcg acgagatggg ggatgtgctt
     4741 gcccgggcgg aggccgaggc gaaacggcgg cagggagctc tgaagtgagc gacttcgtca
     4801 tcgacacgaa tgtcgccgtc gttgcagacc gtcgaggccc gagcaacaca acggaaggat
     4861 gcatcctcgc ggcgatagcg gcactccggc gagttcagaa gagtgaccgc ctcgtgctcg
     4921 atgcggacgg gcgcatcctg gcagagtaca cggagaacgt gaggacggcc ggacaaccgg
     4981 gccccgatca gcgcttcctc aggtgggtcc accgccatca gtacaacgag cagcggtgcg
     5041 tccgagtggc gatccacgtg cgcggagatg aaggtgagga ctacgaggag ttcccggacg
     5101 ccgccgacct gactgacttc gatcggtccg accgcaagtt cgtggcagtg gctctggcac
     5161 gcgggcccgt tacgcccatc atccaagcca ccgatgccga gtggtggcgg cgccgccatg
     5221 cgctggcctc cgctggcgtg aggattgagt tcatctgccc ggatgacatc cagcgctctc
     5281 accgcgccct cctcaacacg cggtagccgc gcgggggcgc aggacatacg gtccacgtgc
     5341 tgaacacgcg agacgaccca gttggggagg tccgcgtggg gagtcctgtt tggaggcgaa
     5401 tcgcatggcc gatacgctgc gcgcgggtgt cattgggtgt ggggtcatgg gtgcagacca
     5461 cgcgcgcctg tatgccgaga tggccgatgt tgagctcgtg gtcgtgatgg atcccgccgg
     5521 cgcccgggcc cgtgagcttg ccggactcca tggctgcgag gtgtgcacgg atctggatac
     5581 ggtggccgag ttcgggctcg atgtggtcag catctgtgtt gatgatcggt
//
LOCUS       DAOSPL010000072         5627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_14539_length_5627_cov_5.351938, whole genome shotgun sequence.
ACCESSION   DAOSPL010000072 DAOSPL010000000
VERSION     DAOSPL010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14539_length_5627_cov_5.351938"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            512..847
                     /locus_tag="PLQ54_02235"
     CDS             512..847
                     /locus_tag="PLQ54_02235"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOS92106.1"
                     /translation="MPALKTAVLTGRHPFNVIGFHELFRRWPGVEPYIQHMVDETYGM
                     NSPGPDSKVLVTTTHQPSMTALAWTRTFKKAPVFCLESGHDNQTWVDANFRRLMTQGT
                     HWTAGREIA"
     gene            973..2223
                     /locus_tag="PLQ54_02240"
     CDS             973..2223
                     /locus_tag="PLQ54_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92107.1"
                     /translation="MGPDPARSPFPILLLALLLAATFVQAADTGRTVDRSALWRRGGV
                     DVLSGFRNMYNFRVIHDPADPRYPYKAWFFGWAAEDGNPYDPGCDAIYTARSAKLSSG
                     WEVYAGPGLWDATGEPRLWVPVVTAQNQPTDQWHNGDPSVVKVGGRYFMAYSSTGFNL
                     DGKLFGTPGDTDGSILCIMGATSGDGVHWRKSPEPILLHQEDLGAPPIPEGEAHLYGS
                     YHRPSLLYDAPARTFRLWFDYWAGPEGGCAMGYAENVGGDFLDSSAWRVVRAGKTPCL
                     PHFPNPNVVRVGTRYHAFADGPVNVGPDAGHPWKARKIIEAVSENGLDWTELGYVEPD
                     ADSPAIHVPEAFVQREGDKTMIYVFYACQNGGEPEYDYRYSRIRWMRRSGDSPRPTPS
                     GAGDIAAVRDADGLETADSRQLPH"
     gene            2747..4207
                     /locus_tag="PLQ54_02245"
     CDS             2747..4207
                     /locus_tag="PLQ54_02245"
                     /inference="COORDINATES: protein motif:HMM:NF016988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOS92108.1"
                     /translation="MPRIPFWPFTPRGASSTGAPIFPIASLAGSAQPDRPSVWPPPEH
                     EERLARYERYRNLYMGRHFEVFHDPLRGADHDLFSQYYVAVNLAAQVSQVAADLLFGE
                     PPQFAAERDSLQGLIDDLVRRNDLISQLQRTARGQSYRGDAVFKARWEPDAETGGRVI
                     LAPLAPDMYFPIFDPDDPSQVKSVWVAWLRPDAQGEPTILRVEVHTPGRIQNLAFPYR
                     NGRLGSPLSLESLSPDLPEEVITGLDEIPLVHIPNAQTDDSGPFGISDYAFFEPIQRE
                     INNRLSQIGTVLDKHADPNMVIPLQALDAQGEFRAVTQKAWPIDQGDPRPEYITWDGK
                     LESAFRELEMLEEFFFLASGVSPSTLGVSTGVGFGGSTESGRAIQLRQIRTLKWIENK
                     RASWTTGLKRLFRIAQKLLIAHGGFPSGERAEPTPVQILWRDGLPTDRAADIDDAVHA
                     VQGGVMSLRRGIQLAQGLEGEGLASEEAQIRADRGA"
     gene            4281..4703
                     /locus_tag="PLQ54_02250"
     CDS             4281..4703
                     /locus_tag="PLQ54_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92109.1"
                     /translation="MSDTMQEYPSDETPDTLPEAVQSRLAELEQQAETQRAELARARA
                     ESVKAQMLLEWPERLPGAYQAQVVASENAEEVKESLRQVTEQFQADRKAFGSAARPVG
                     APPAGPGAVAHPRWHGKRPSEISPRDWPTYKRQVLGLR"
     gene            5168..>5627
                     /locus_tag="PLQ54_02255"
     CDS             5168..>5627
                     /locus_tag="PLQ54_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92110.1"
                     /translation="MAVTTTTDIDDIIEIALAEARYTVQANTFVQNLVTVKDISGSPA
                     KSARFPKFGAITATQVEEGTDYTSHADLATSSVEIPVKEVVAGTIITDLAKTSSREDI
                     GIAAGRVLGNAIAQYIEQDLIGLFADFTTNQVGTTNVDITIPNWIKAKTLL"
BASE COUNT         1030 a         1991 c         1730 g          876 t
ORIGIN      
        1 gggcgagggg gactacagcg ccgagcccgg cacgggctcc ttccgctcga cccccgaggt
       61 cccgctcaag atcgacgatc actgcatgga tgccctgcgc tatgccgtga tgggtctcta
      121 ccggccggcc tctcccggcc ccatccccaa gcccaccggg tggtagggga ggtgagatgt
      181 ctgcaatacc cggggaggga ccgaatggaa ggggcccgcg aagcatgaag ggcgccatcc
      241 ggcccggtct tgatcacgcg aagctgccac tccccgaagg gagactcatg cccgctgact
      301 acgagaccag accagacggc tccgtgcagc tcgccgtgtt ctccgggccg aatgacctgt
      361 tcacatgctc agcttgtcgg cgactgcatg ggcatgcgtt cgcatcctgg agatcgatgg
      421 aaccgaggag cgctgaggaa cccgcaccgc ccacggtgaa cctactcgcg ttgccagaaa
      481 agactctcag cctccaggga gaccacccac catgcccgcc ctcaagaccg ccgtcctcac
      541 cgggcggcat cccttcaatg tcatcggctt ccatgagctg ttccggaggt ggccgggggt
      601 cgagccctac atccagcaca tggtcgacga gacctatggc atgaacagcc cggggccgga
      661 cagcaaggtt ctggtgacca ccacccatca gcccagcatg accgccttgg cctggacgcg
      721 caccttcaag aaggctcccg tgttctgcct cgaatcggga cacgacaacc agacatgggt
      781 cgacgcgaac ttccgccggc tgatgaccca ggggacccac tggaccgcgg ggagggagat
      841 agcgtgaggg agagagtccc aaacgggccg gatggcgccc ttcccgcttc gcgggcccct
      901 tccatgcggc ccctcctcgc gctctacgag ggtcttcgga ggggccgaat agggaggatc
      961 ggccgcaggc cgatgggacc cgatccggcc cggagtccgt tcccgatcct gctcctcgct
     1021 ctgctcctcg cggcaacctt cgtccaggcg gccgatacgg gccgcacggt ggaccggtcg
     1081 gcgctctggc ggcggggtgg agtggatgta ctgtccggct tccggaacat gtacaacttc
     1141 cgggtcatcc acgaccccgc ggaccccagg tatccctaca aagcctggtt ctttggatgg
     1201 gccgcggagg acggaaaccc ctatgacccc ggctgcgatg ccatctacac cgcccgcagc
     1261 gcgaaactct cttcgggctg ggaagtctat gccgggcccg gtctgtggga tgcgaccggc
     1321 gaaccgcgcc tctgggtccc cgtggtcacc gcgcagaacc agcccaccga ccagtggcac
     1381 aacggtgacc cttcggtggt gaaggtcggc gggcggtact tcatggccta cagctccacc
     1441 ggcttcaacc tggatggcaa gctctttggt acccctggcg acaccgatgg cagcatcttg
     1501 tgcatcatgg gtgcgacctc gggcgatggc gtccattggc gcaagtcacc ggagcccatc
     1561 ttgctccacc aagaagacct gggcgcccct ccgatccccg agggcgaggc gcacctgtat
     1621 ggttcctacc atcggccctc gctgctctac gatgccccgg cgcgcacctt ccggctgtgg
     1681 ttcgactact gggccggacc cgagggcggc tgtgccatgg gctatgccga gaacgtgggc
     1741 ggcgacttcc tggactcgtc ggcatggcgt gtggtgcgcg cgggaaagac accttgtctg
     1801 ccgcacttcc cgaacccgaa cgtggtccgt gtcggtaccc gctatcacgc cttcgccgat
     1861 ggaccggtga acgtgggccc cgatgcgggg cacccgtgga aggcgcggaa gatcatcgag
     1921 gcggtttcgg agaacggact ggactggacc gagctgggct acgtggaacc tgacgccgac
     1981 tcaccggcca tccatgtgcc ggaggctttc gtccaacgcg agggcgataa gacgatgatc
     2041 tacgtgttct atgcctgcca gaacggcggg gagccagagt acgactaccg gtatagccga
     2101 atccgctgga tgcgccgatc gggggactcg ccgcgcccta cgccttcagg cgccggagat
     2161 atcgcagccg ttcgtgatgc cgacgggctt gagaccgccg attccagaca actcccgcac
     2221 tgaccagggc gccaaccgca gcgagacatt ccatcgtcat gagtagttcg ggcaccagag
     2281 ccacctccgt cactcggctt ccagtctccc ccggatcatg cccgattatg gttccgccgc
     2341 ttggcggcca gggttagggg tctgacaagg ctggagcaga gctttgtcgt cctcacccag
     2401 ccgcaaagct ctgctcccca gacaggttca ttttagttcg ccaaaacagt aaagagaacc
     2461 acggccaagg cactgccaca cagcaccgcg aaaacatcgc cgacacacgt caagaccttg
     2521 ctcgccaaca tgcccaccca cccccaagta tctacggcga atcgttcctc tggggtaggg
     2581 cagattcacc cggctcggcg cctttgttga ggctccatct gagcccgaga cgccaccttt
     2641 caaaagtctt cgacgcaccg taacgcggca ggttcatttc ggagggagaa gagtccgtac
     2701 ctccagcgtg atcgcttgcc caccacccca catcccggag gcccccatgc cccgcattcc
     2761 cttctggccc ttcacgccgc gtggcgcttc cagcaccggc gccccgatct ttccgatagc
     2821 ttccttggcg ggatcggccc agccggaccg gccttccgtc tggcccccgc ccgaacacga
     2881 ggaacgcctg gctcggtatg agcgataccg gaacctctac atgggccgtc acttcgaggt
     2941 cttccacgac cccctccgcg gcgccgacca cgacctcttc tcccagtact atgtcgccgt
     3001 caacctggct gcccaggtca gccaggttgc cgccgatttg ctcttcggcg aaccgcccca
     3061 attcgcggcc gagcgggact cgctgcaggg cctcatcgac gatctggtca gacgtaacga
     3121 cctcatcagc cagctccagc gaaccgctcg gggccagtcc taccgcggcg atgccgtctt
     3181 caaagcccgt tgggaacccg atgctgagac cggcggccgc gtgatcctgg cccccctggc
     3241 ccccgacatg tacttcccca tcttcgatcc cgatgacccg agccaggtca agagtgtctg
     3301 ggtggcctgg ttgcgccccg acgcccaggg ggagcccacc atcctccgcg ttgaggtcca
     3361 cacgcccggc cggatccaga acctggcctt cccctatcgg aacgggcggc tgggcagccc
     3421 gctatcgctc gagagcctct ctcccgatct gccggaagag gtcatcaccg ggctggatga
     3481 gattcccctg gtgcacatcc caaacgccca gaccgatgat tcgggcccct ttgggatcag
     3541 cgactacgcc ttctttgagc ccatccagcg agagatcaac aaccgcctga gccagatcgg
     3601 cacggtgctg gacaaacacg ccgacccgaa catggtcatc ccgctccagg cgctggacgc
     3661 ccagggcgag ttccgcgcgg tcacccagaa ggcgtggccc atcgaccagg gcgatccccg
     3721 gcccgagtac atcacctggg atggcaagtt ggagagcgcc ttccgggagc tggagatgct
     3781 cgaggagttc ttcttcctgg cctccggcgt ttcgccgagc actctgggtg tctccaccgg
     3841 ggtgggcttc ggtggcagca ccgagagcgg ccgagcgatc cagctccgcc agatccgcac
     3901 cctcaagtgg atcgagaaca agcgggcgag ctggaccacg ggcctgaagc ggctattccg
     3961 catcgcccag aagctgctca tcgcccacgg gggcttcccg agcggcgagc gcgcggaacc
     4021 cacgccggtg cagatcctct ggcgtgatgg cctccccacc gaccgtgcgg ccgacatcga
     4081 cgacgccgtg cacgcggtcc agggcggcgt catgagcctc cgccgcggca tccagctcgc
     4141 tcagggcctc gagggcgagg gcctggcgag cgaggaggcc cagatacgag cggaccgggg
     4201 ggcctgagcc tcagccctaa ggccgcgccg tcggcctgag acccggcaag caacagaccc
     4261 caaggaaagg cagagacccc ttgagcgaca ccatgcagga atacccatcc gacgagacgc
     4321 cggatactct ccccgaggca gtccaaagcc gcctggccga gctcgagcaa caggccgaga
     4381 cccagcgggc ggaactcgcc cgagcgcgag cggagagcgt gaaagcgcaa atgctactgg
     4441 agtggcccga acggctgccc ggcgcctatc aggcccaagt ggtggccagt gagaacgccg
     4501 aggaggtcaa agagagcctc cgccaagtca ccgagcagtt ccaggccgat cgcaaggcct
     4561 tcggctccgc cgcccggccc gtgggcgctc cgcccgcagg tcccggcgcc gtcgcccatc
     4621 cccgatggca tggcaagcgg cccagcgaga tctcgccccg agactggccg acctacaagc
     4681 gccaggttct ggggctgagg tagagcaagg ccccactggc gctaagttag gcccgagatg
     4741 acgccgcagg agcaccgaga cggcacgtct ggatctggcg gaggggcctg ataggggcat
     4801 tggcggtaac tgagggctcc agcggcccat gggcctggtt gcggaggggc gccatggggg
     4861 gaaccacgcg ggctgtgcgt ggtgggcccc caacgcgccc attccagggt cccgagtgcg
     4921 gcgcgaagcc gcattcgggc tcgatccgtc ggcctgcggc caaccggggc cgattagggt
     4981 cccaccaagc ttcgcttggt cccccctatc aggcccctcc gcaacaccgg gtggtgccga
     5041 acccaggccc tccggctcgg aacccagccc taagttgccg ccagtacccc cttccggccc
     5101 ctccgaagat ccgccataca tcccccgcaa cccccgcacg ttccccatcc ccgaaaggag
     5161 tcgccccatg gcagtcacca ccacgaccga catcgacgac atcatcgaga tcgccctggc
     5221 ggaagcccgt tacacggtcc aggccaacac cttcgtccag aacctggtta cggtgaagga
     5281 catctcgggg agcccggcca agtcggcacg cttccccaag ttcggcgcca tcacggccac
     5341 ccaggtcgag gagggcacgg actacaccag ccatgcggac ctggccacct cgagcgtcga
     5401 gatcccggtc aaagaagtcg tcgccgggac catcatcacc gacctggcca agacctcgag
     5461 ccgcgaggat atcggcatcg ccgccggccg cgtgctgggc aatgccatcg cccagtacat
     5521 cgagcaggac ctcatcggct tgtttgccga cttcacgacc aaccaggtcg ggaccacgaa
     5581 tgtggacatc accataccca actggatcaa ggccaagacc ctcctgg
//
LOCUS       DAOSPL010000073         5611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_14582_length_5611_cov_2.655148, whole genome shotgun sequence.
ACCESSION   DAOSPL010000073 DAOSPL010000000
VERSION     DAOSPL010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14582_length_5611_cov_2.655148"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..886
                     /gene="hpt"
                     /locus_tag="PLQ54_02260"
     CDS             275..886
                     /gene="hpt"
                     /locus_tag="PLQ54_02260"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOS92111.1"
                     /translation="MVTIGEERAAAIERELEVLIPASEIARRVGELAAEIQSSLAGER
                     VVAVSALIGGFVFLSDLIRQIRPAPTVDLAHLRSYGTGTRSSDHITLLRPLDQPIRGR
                     HVLIVEDIVDTGQTARFLIDMVRAESPAAMTFVTLLNKPARRKVHIEPDLVGFEVTNR
                     FVVGYGLDYAGLFRSLPDVCALPRWAEDAWPDGSPPGEARAPR"
     gene            complement(1343..4861)
                     /locus_tag="PLQ54_02265"
     CDS             complement(1343..4861)
                     /locus_tag="PLQ54_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92112.1"
                     /translation="MARPTRHSGCLVALAACACMVCATEAPAQEWTCIDGFEGGARVW
                     TDSAGPGPRVRVTDRAAHGGLRSLALGATLANAGRSIRVSCETDIGDLQWSLESRVRF
                     WLRGDERAQMPHGGWIFVEAHGGPGGADSHWMLDIPGDTYRDPSWHAIELGPVSTAHN
                     PDWAPDADGRPEPRAFYRIILVAQQEAPPELNVPFTFVIDDVEATHVKPWDVRHVPAR
                     MEDRPDHVTPIGRGFTGRRREHPATVTFEDASGWLAGAIGPGSVELVISSEEPCYEDL
                     KHQAKVTYSSPGGPGVFELVPPRPIPIRGEANAACAWVYGNNWSWMPDPGTPPVTVWV
                     RVIDATGQRHRINMGVVNYRFYGMLHKRLRDHPRGDPGHRFAGGASDGRIHAPASVEA
                     IEIHGGTNPEPRTIYIESVSLFRDDMPLPAFRPELVENLPFPTTPDTILPPVAKPTRV
                     SLERQGEAYVFTLEGDERIVGRYTPRTGTLTDLSVQVGDREPYIPCAGAGPAFDLGGD
                     ERDPEDRFLTRQLVAVSPRPGAVTTRWRVGIGGEWADYSLTLRAKGKSFIVDWSSEGT
                     QATALRLGHASGLKSPRLVRIPYMAIYGEGPAVLLDDGVFTLTLLDWYQTGCSALYPS
                     MGLGTAGEATMNGGSLYGTRTDGKRNPLRERQFISVSTTLDEVLPTIPNPPSDQAEVL
                     RSRLYTHLGGTAPSRFDDWLAMWRQYHRRGIRNLLVTHHEDAWTNGADVGQGPQEYTM
                     CIEAAPEVGDDTMRAYCRAMRAMGYYVGLYENFTDYNPLGKSWDERNAGRNAQGELVR
                     VWPPTYAIRPLKALEMARDYPRRVHAKFGCNTAYRDCHTAYTPWGQVDYQAGAPGAGL
                     LSANFRAWGALLMDGSDAYGGPVFSEGLHHWFSAGLVDGSYGQINTPSASEYPLLLDF
                     DLRRLHVLGADISMTPGWNWGRGVYHCLAATIAYGHMGFQPFGDTALAGRYYYLMQQL
                     QSRYVMIPATQVLYHRAGRMVPISEALKQGDPLQSQVRVTYANGLEVYANCNPTSRWP
                     VEVGGQRRDLSPDSWAAVLGDEFEEYCTEVDGVRIGYVRSPAYLFADGGGTWHDFAAL
                     ATDGAVAVLADAGCGREVVPFTAASRIIVATDGPVRVEALDESGRLMGVVPSAMVDGR
                     VAFVPVDGAVSYRLRPRGHPGPKRAR"
     gene            complement(4854..>5611)
                     /locus_tag="PLQ54_02270"
     CDS             complement(4854..>5611)
                     /locus_tag="PLQ54_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOS92113.1"
                     /translation="TDQSIQVSPRLVVWPSGLVDAPRGADDPAWAAIGASARELDTTL
                     VAPARAAPGVPETAYVFGRDGQLLGQRPRSEVVPLAGGGPFLRSGTVLTNPARLGLML
                     GWEMEVPALACYQVRDDAELLVASASGRRDIGRAGRAQRAAAGRMRAVETRRFVVQVN
                     GFGLSQIVDPYGREQLRTGVGAIWAVGYAGTISGYTPYVIWGRWVGPASAALAAAVLL
                     GAALGALWRAAGRRPDATSADAGPPANGGEADG"
BASE COUNT          832 a         2012 c         1956 g          811 t
ORIGIN      
        1 aaccgctcgg accggccatc ctgcaggaac acccccgagg ccgtcgactc acccgctcgg
       61 gcatcggcga aggcagcccc ccgccgcttg gcctcatcgc agacccagcg aaccaggtcc
      121 ttcatggccc cgaccccctt atccatctcg gacgaacggc caggacgctt ccccaccggc
      181 cgcgggcatc cctccacggg aacggcggct gggaacgccg ggagggcagg tgacggcgct
      241 tccacaggga acctggcccg cagggaggga caccatggtc accatcggcg aggagcgcgc
      301 ggccgccatc gagcgggaac tcgaggtgct gataccggcc agcgagatcg cccggcgcgt
      361 gggcgagctg gccgcggaga tccagtcatc cctcgccggc gagcgggtcg tggccgtgtc
      421 cgctctcatc gggggcttcg tcttcctctc cgacctgatc cgccagatcc ggcccgcccc
      481 caccgtcgat ctggcgcacc tgcgctccta tggcaccgga acgcggtcgt cggaccacat
      541 cacgctgctg cgcccgctcg atcagcccat ccggggccgt cacgtgctga tcgttgagga
      601 catcgtcgac acgggccaga ccgctcgctt cctcatcgac atggtccgcg cggagagtcc
      661 ggcggccatg accttcgtga cgctcctcaa caagccggcg cggcgcaagg tgcacatcga
      721 gcccgacctg gtggggttcg aggtaacgaa ccggttcgtg gtgggctacg gcctggacta
      781 cgccggcctg ttccggtcgc tccccgacgt gtgcgccctt cccaggtggg ccgaggacgc
      841 ctggccggat ggctcgccgc cgggcgaggc gcgcgcgccg aggtaagtcg ccccagccgc
      901 cgctcggggc ccaaagcgct cgaaacgtct cctgctaagt cttttggctt gacatggcgg
      961 ccctaatatg gtataatgca aacatgtgtg tgacactaac gcccgccgag ccaatccgcg
     1021 cgccgcaccg gttctccccg tcctccaccg cctgggccga ggccaccggt gagcatgccg
     1081 tgagccccgc ccggcccggg cgggagttcg acgaacgcag gtgggtcatg aaccactact
     1141 cgcccgtcgc ctcccgtagc cgcttgtccg gggaaggtct cgcgcgcgtg ttcaccaccg
     1201 gaagtgtgga gcgtggagcg ttgtggaggg tttctgggta tttttggcgc gaggccgcgt
     1261 tcagacgaaa aacacccaag aacgctccac aaccctccac accctccaca cttcgcccca
     1321 gccggcctcc aagggatcct cgtcagcgcg cccgcttcgg gcccggatga ccccggggcc
     1381 tgagccggta cgacacagct ccgtccacgg gcacgaacgc cacccggcca tcgaccatgg
     1441 cgctcgggac gacgcccatc agcctcccgg actcgtccag ggcctcgacc cgcaccgggc
     1501 cgtcggtcgc cacgatgatg cgggaggcgg ccgtgaacgg gacgacctcg cgcccgcagc
     1561 cggcatcggc cagcaccgcc acggcgccat cggtggccag ggcagcgaag tcgtgccacg
     1621 tcccgccccc gtcggcgaag aggtacgccg gcgaccggac gtagccgatc cgcacgccat
     1681 cgacctcggt gcagtactct tcgaactcgt cgccgagcac ggcggcccac gagtcgggtg
     1741 acagatcgcg ccgctggccg ccgacttcca ccggccagcg gctggtcggg ttgcagttgg
     1801 cgtacacctc gaggccgttc gcgtaggtga cccgcacctg gctctgcagc gggtcgccct
     1861 gcttgagggc ctcactgatg ggcaccatcc gcccggcgcg gtggtagagc acctgggtcg
     1921 cggggatcat cacgtaccgc gactggagct gctgcatcag atagtagtaa cggcccgcca
     1981 gcgccgtgtc gccgaagggc tggaagccca tgtggccgta ggcgatggtg gccgccaggc
     2041 agtggtagac gccccggccc cagttccacc ccggcgtcat cgagatgtcg gctccgagca
     2101 cgtgcaggcg ccgcaggtcg aagtccagga gcaacgggta ctcgctggcc gagggcgtgt
     2161 tgatctgacc atagctgccg tccaccagac ccgcggagaa ccagtggtgc aggccctcgc
     2221 tgaagaccgg accgccgtag gcgtcgctgc cgtccatcag cagggcgccc caggcgcgga
     2281 agttggccga caggagcccg gccccgggcg cgccggcctg gtagtcgacc tggccccagg
     2341 gcgtgtaggc cgtgtggcag tcacggtagg ccgtgttgca cccgaacttg gcgtggacgc
     2401 ggcgcgggta gtcccgcgcc atctccagag ccttcagcgg ccggatggcg tacgttggcg
     2461 gccacacccg gaccagctcg ccttgggcgt tccggccggc gttgcgctca tcccacgact
     2521 tcccgagcgg attgtagtcg gtgaagttct catagaggcc cacgtagtac cccatggcgc
     2581 gcatggcccg gcagtaggcg cgcatcgtgt cgtcgcccac ctcgggagcc gcctcgatgc
     2641 acatggtgta ctcctgcggc ccttggccca catcggcccc gttggtccag gcatcctcgt
     2701 gatgcgtcac cagcaggttg cggatcccac gccggtgata ctgccgccac atggccagcc
     2761 agtcatcgaa ccggctgggc gccgtgcccc ccagatgggt gtacaagcgg ctccggagta
     2821 cttcggcctg gtccgacggc gggttcggga tggtcggcag tacctcgtcg agcgtggttg
     2881 agaccgagat gaactgccgc tcgcgcagcg ggttccgctt cccatcggtt cgcgtgccgt
     2941 agagcgagcc gccgttcatg gtggcctcgc ctgcggtccc caggcccatg gaggggtaga
     3001 gggccgagca gcccgtctgg taccagtcca gcagcgtcag cgtgaagacg ccgtcatcga
     3061 gcagaaccgc cggaccttcg ccgtagatgg ccatgtaggg gatacgcacc agccgagggg
     3121 acttcagccc cgacgcgtgt ccgagccgga gggcggtggc ctgggtgccc tccgatgacc
     3181 agtcgacgat gaaggacttc cccttggccc gaagggtgag cgagtagtcg gcccactccc
     3241 ccccgatgcc cacgcgccac cgggtggtca ccgcgcccgg gcgcggcgag acggccacca
     3301 gttgccgcgt caggaaccga tcctccgggt cgcgctcgtc gccgccgagg tcgaaggcgg
     3361 gccccgcgcc ggcgcagggg atgtagggct cccggtcgcc gacttgcaca gacagatccg
     3421 tgagggtgcc cgtgcgcggg gtgtagcggc ccacgatgcg ctcatcgccc tcgagggtga
     3481 acacgtaggc ctcgccctgc cgctccaggc tcacgcgggt cggcttcgcc accggcggca
     3541 agatggtatc cggcgtggtc gggaacggca gattctcgac gagctccggc cggaaggccg
     3601 gcaggggcat gtcgtcgcgg aacagcgata ccgactcgat gtagatagtt cgaggttccg
     3661 ggttggtgcc gccgtggatc tcgatggcct ccaccgaggc gggggcgtgg atccggccgt
     3721 cggacgcacc accggcgaac cgatggcccg ggtcgcctcg ggggtggtcc cgcagccgct
     3781 tgtggagcat gccgtagaat cggtagttca ccacgcccat gttgatgcgg tgccgctgcc
     3841 cggtggcgtc gatgacccgg acccagacgg tcacgggcgg ggtgcccggg tcgggcatcc
     3901 acgaccagtt gttgccgtag acccacgcgc aggcggcgtt cgcctcaccg cggatcggga
     3961 tggggcgggg gggcaccagc tcgaacacgc ccggcccgcc cggactggag tacgtgacct
     4021 tggcttggtg cttcaggtcc tcgtagcacg gctcctcgga tgagatgacc agctcgaccg
     4081 accccggccc gatggcgccg gcgagccagc ccgaggcgtc ctcgaaggtc acggtggcgg
     4141 ggtgctcgcg cctgcggccg gtgaaacctc gcccgatggg tgtgacgtga tcggggcggt
     4201 cctccatccg ggcggggacg tggcggacgt cccacggctt cacgtgagtc gcctccacat
     4261 cgtcgatgac gaaggtgaac ggcacgttca gctccggcgg cgcctcctgc tgcgcgacca
     4321 ggatgatccg gtagaaggca cggggctcgg gtcggccgtc ggcatcgggc gcccaatcgg
     4381 ggttgtgggc cgtgctcacg ggcccgagct cgatcgcgtg ccaggacgga tcgcggtacg
     4441 tatcgcccgg gatgtcgagc atccagtggg agtcggcgcc gccggggccg ccgtgggcct
     4501 ccacgaagat ccaccctccg tgaggcatct gggcgcgctc atcgccccgg agccagaacc
     4561 ggacgcggct ctcgagcgac cactgcagat caccgatgtc cgtctcgcac gacacacgga
     4621 tactgcggcc cgcattggcg agtgtggcgc cgagagccag cgagcgcagc ccgccatggg
     4681 ccgcgcggtc ggtcacgcgc acccggggcc ccgggccggc gctgtcggtc cagacgcgag
     4741 caccgccctc gaagccgtcg atgcaggtcc actcctgcgc gggggcctcg gtcgcacata
     4801 ccatgcaggc gcacgccgcg agggcgacga ggcacccgct gtgtcgcgtc ggcctagcca
     4861 tcggcctctc ctccgttcgc gggcggcccg gcgtccgcgc tcgtggcatc cggccgccgt
     4921 ccggcagctc tccacagggc gcccagcgcg gccccgagca gcaccgcggc ggccagagcg
     4981 gccgatgccg ggcccaccca gcgtccccag atcacatagg gcgtgtagcc gctgatcgtg
     5041 ccggcgtagc ccacggccca gatggccccc acgccggtgc ggagttgttc tcgcccgtag
     5101 gggtcgacga tctgcgagag tccaaacccg ttcacctgaa cgacgaagcg gcgggtctcg
     5161 acggcccgca tccgcccggc ggcggcgcgt tgggcacggc ccgcccggcc gatgtcgcgg
     5221 cgaccgctgg cgctggccac gagcagttcg gcatcgtcgc gcacctggta gcacgccagt
     5281 gccgggacct ccatctccca gccgagcatc agccccagcc gggccgggtt ggtgagaacc
     5341 gtgccggatc gaaggaaggg cccgccaccg gccaggggca cgacctcgct gcggggccgc
     5401 tggcccagga gttggccgtc gcggccgaag acgtaggctg tctcgggaac gcccggtgcg
     5461 gcccgcgcgg gggccacgag agtggtgtcc agctctcgcg cgctggcccc gatcgccgcc
     5521 catgccgggt cgtccgcccc ccgaggggcg tcgacgagcc ccgagggcca caccacgagc
     5581 cggggggaca cctggatgct ctggtccgtg a
//
LOCUS       DAOSPL010000074         5579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_14688_length_5579_cov_3.203114, whole genome shotgun sequence.
ACCESSION   DAOSPL010000074 DAOSPL010000000
VERSION     DAOSPL010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14688_length_5579_cov_3.203114"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PLQ54_02275"
     CDS             <1..595
                     /locus_tag="PLQ54_02275"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOS92114.1"
                     /translation="GCDAVKVGIGPSAICTTRPVTGAGVPQLTAIIDCARAASEHDVP
                     IIADGGVKYSGDITKALAAGAETVMIGSLFAGTDEAPGETILFEGRTYKEVRGMASIA
                     AMKEGSSDRYFQDHVETDTKYVPEGIEARVPHKGALATLVYYLVGGLRSGMGLVGASD
                     LAELREKAVFMRVTSAGLRESHVHDVAISKESPNYQR"
     gene            948..1583
                     /gene="sigH"
                     /locus_tag="PLQ54_02280"
     CDS             948..1583
                     /gene="sigH"
                     /locus_tag="PLQ54_02280"
                     /inference="COORDINATES: protein motif:HMM:NF006148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HOS92115.1"
                     /translation="MADAERSCFDRMPDELVVSLGKSGDVAATEYLLRKYRSVVLYKS
                     RNYFLHGADRDDMIQEGMVGLFKAIRDFRADRACSFRTFADVCITRQMITAVKSSTGQ
                     RQLPLTYASSLSPGSRDDGAMDRLDVPADADVVDPEQLLIARETVRELRSVLRQNLSE
                     FEWNVFVGFSQGKCYQEIADEQGSTPKSVDNALCRVRRKVQRFRAQSMAVS"
     gene            1762..2946
                     /locus_tag="PLQ54_02285"
     CDS             1762..2946
                     /locus_tag="PLQ54_02285"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOS92116.1"
                     /translation="MSRRRGLCVFVVLVFAGLALTPSARAADIARWRQKYPLEKEVEI
                     GRKTAEEIDRQYGVLDDPEQLARIQAIVNEIVAVSDRPDLQCDVKILSVPVPNAMAIP
                     GGFIRCTSGLLDMCNSDDELAAVLAHEIAHMCNYHGMRQDERDRKMGIYQLGLQLAAI
                     VAALNGADPGFVQYAVAAGYFAHMQIIVGYSREYEVEADRDGLAYLMKTKYNPTGMLT
                     TMERFADLATRTWQGSDALRDFDTHPPSAARVAEVERYLREANVNINRAVVLRGYRAV
                     PEEIEYAPGQRTWQICFGGRPVFQASPEPIDGKTAEERVHAIATSMNMVISVSGLAIH
                     TVGLSDRKGYPEISFSQYPVLRILEQDAAFMGMDAKAYASLVREQLIYALDQAGREAL
                     RT"
     gene            complement(2943..4160)
                     /locus_tag="PLQ54_02290"
     CDS             complement(2943..4160)
                     /locus_tag="PLQ54_02290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS92117.1"
                     /translation="MSAVPSILHLAFGESLRDQQFVTELCEAVVRRSGLGRSQAVISD
                     TNRALLRRTARLKARTAAVRLTGRRRHDRRAMAQAVAELGDLAPQLVHAHGFAAAAWA
                     EALARRYRVPWTMSVLSPVPDHWQNRRQIWRASALAALSLDALPRGLRNRATQIPGGL
                     DKREFVPSAVAEMLMAELGLNPYTLHLGLVGQFDDRRSGWADFLEAAARVARRVPLVE
                     FVIVGSGRDSAAMEHYAHQSGILGNTRFLGLRIDRSRIYHALDVVVLPSHETLFPWEL
                     LEAAAAGAPVIATAIEPHQRMAAGDPSVTFYPAGDTAALADRLLAVVEGTGPGLSSDM
                     VDLSLLGEGLHGVVRARISQTHYNVGDDELEELVDPDAPDTLRRRVMRDMEITQIAKR
                     YEALWDGVVASVR"
     gene            complement(4293..5306)
                     /locus_tag="PLQ54_02295"
     CDS             complement(4293..5306)
                     /locus_tag="PLQ54_02295"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS92118.1"
                     /translation="MAQLKVGLLGCGGMGLSLATPCHELPNSTIAAVCDTDEARAREA
                     GDKFAADVYTDYGRLVAEAGVDAVIVASPPFMHRPMVECAVASGKHVLCEKPMAPTVA
                     DCNAMIAAADRAGTQLMVAQVLRYIPAFHWLRDQIGSGAYGDVLAISIERIGGRWGGD
                     YGVPWRLRLAEAGGNLMEINAHELDFMRCLAGDAARVYAVGGNYICDEQDYEDLVFAS
                     ITFKSGAVGWLHSSSLCHAGMGAYTGRVQCSKGTFFIENGFSSDAYIRYGVEGGDEVK
                     AQKIGDIEVEPAVQHEVRLFVEAVLSDTPVAVPGSDGRAAVELAEAAYRSIAERRPID
                     LPL"
     gene            complement(5390..>5579)
                     /locus_tag="PLQ54_02300"
     CDS             complement(5390..>5579)
                     /locus_tag="PLQ54_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92119.1"
                     /translation="HAIHDYAERMKAARPIPRPDAWIEIDRYKHANGIQGLYADVKDA
                     FWRAVDEGKTPGLRWREP"
BASE COUNT          984 a         1909 c         1786 g          900 t
ORIGIN      
        1 ggggtgtgac gcggtcaaag tgggtatcgg tccctcggcg atatgcacga cccgtcccgt
       61 gaccggcgcg ggcgtgcccc agctcacggc catcatcgac tgcgcccggg cggcctctga
      121 gcatgacgtg cccattatcg ccgatggggg cgtcaagtac tcgggcgaca tcaccaaagc
      181 attggccgcg ggagccgaga ctgtcatgat cggttccctg ttcgccggta ccgacgaggc
      241 tccgggcgag acaatcctct tcgagggccg cacctacaag gaggtccgtg gcatggcctc
      301 catcgcggcc atgaaggagg gctcgtccga ccgatacttc caggaccacg tggagacgga
      361 caccaagtac gtgcccgagg gcatcgaggc ccgggttccc cacaaaggtg cgctggccac
      421 cctcgtgtac tacctggtcg gcggattgcg ctccggcatg gggctggtgg gggccagcga
      481 tctggccgaa ctgcgcgaga aagccgtctt catgcgggtc acgtccgcgg gtcttcgtga
      541 gagccacgtc cacgacgtgg ccatcagcaa ggagtcgccg aactaccagc ggtgagtggt
      601 gtcagatacc ggaagcatat tccggtcatg cgggtcctca ttcccaccat cggtgggctg
      661 tggataacct cagccatggc ttgcaggaag cgcgtttgag ctactggggc gtcggggttc
      721 ggttcgacaa agggccggac gcaagctgcc ggatgcctga cccggaaggg cactgccacc
      781 agttgccgct tcggcgggtt cgctccccgg aggcccaccc caccgatttc ggaccgcgat
      841 tgacaactac aaggacgttt gtgacccctt gttattccgc ttgacacccc ctgtacggcg
      901 gcggtacact cccgccgttc tgatcacggg atccggggga tgcacctgtg gccgacgccg
      961 agagaagctg cttcgatcgc atgcctgacg agctcgtcgt atcactgggt aagagcgggg
     1021 atgtcgcggc gaccgagtat ctgctccgga agtaccgatc cgttgtcctc tacaagtccc
     1081 gcaactattt cctgcacggt gccgaccggg atgacatgat ccaggaggga atggtcggcc
     1141 tgttcaaggc gatccgcgac ttccgggcgg accgcgcctg ttcgttccgc accttcgccg
     1201 atgtctgcat cacgcggcag atgatcaccg cggtgaagag cagcaccggc caacgccaac
     1261 tgcctctcac ctacgccagc tccctgagtc cgggctcgcg ggacgacgga gccatggacc
     1321 ggctagacgt gcccgccgac gccgatgtgg tcgaccccga gcagctcctg atcgcccggg
     1381 agaccgtccg cgagcttcgc tcggtgctcc gccagaacct gagcgagttc gaatggaacg
     1441 tcttcgtcgg tttctcccag ggcaagtgct atcaggagat cgcggacgag cagggcagca
     1501 cgcccaagtc ggtcgataac gctctgtgcc gcgtccggcg taaggtccag aggttccgag
     1561 cccagtccat ggcagtgagc tgatcgcggg cttcgggcct tcggacgcgt ccgggtccgg
     1621 cgccgcaccc cgagcggcgg ccggaatggc tgccgcaccc cgcccatctg ccacgttttg
     1681 gagcgaacgc ctcgtggcag accagcgtct ggatatgtga agccaatgct gtggcgtggg
     1741 tgccggcgtg gaggggttcg cgtgtctcgc cgtcgtgggt tgtgcgtgtt cgtcgtcctc
     1801 gtgttcgccg gcctggccct gacgcccagc gcccgagcgg ccgacatcgc tcgttggcgc
     1861 cagaagtatc cgctcgagaa ggaagtcgag atcggccgca agacagccga ggagatcgac
     1921 cgccagtacg gtgtcctcga cgaccccgag caactggcgc gaatccaggc catcgtcaat
     1981 gagatcgtgg ccgtatccga ccggccggac ctccagtgcg atgtgaagat cctcagtgtc
     2041 cccgtaccca acgcgatggc aatcccgggt ggcttcattc gctgtacttc ggggctgctg
     2101 gatatgtgca actcggacga cgagctcgcc gcggtgctgg cgcacgagat cgctcacatg
     2161 tgcaactacc atggcatgag gcaggacgag cgcgaccgca agatgggcat ctaccagctc
     2221 gggctgcaac tggcggccat cgtggctgcg ctcaatggcg ccgatcccgg cttcgtgcag
     2281 tacgccgtcg ccgccgggta cttcgcgcac atgcagatca ttgtgggcta cagccgcgag
     2341 tacgaagtcg aggccgatcg cgacggcctg gcctacctca tgaagaccaa gtacaacccc
     2401 acgggcatgc tgacgaccat ggagcgcttc gccgatctgg ccacgcggac gtggcagggc
     2461 tccgacgcgc tccgggactt cgacacccat cccccctcag ccgcgcgtgt cgccgaagtc
     2521 gaacgctatc tgcgggaagc caatgtgaat atcaaccggg ccgtcgtcct ccgaggctat
     2581 cgtgccgtgc ccgaggagat cgagtacgcc ccgggccagc gaacctggca gatctgcttc
     2641 ggcgggaggc cggtcttcca ggccagcccc gaacccatcg acggaaagac ggctgaagaa
     2701 cgcgtccacg ctatcgccac cagcatgaac atggtgatct ccgtcagtgg cctcgccatc
     2761 cacaccgtcg gcctctcgga ccggaagggg taccccgaga tcagcttcag ccagtacccc
     2821 gtgctgagga ttctggagca ggatgccgcc ttcatgggca tggacgccaa agcctacgcc
     2881 tcactggtcc gcgagcagct catctacgca ctcgaccagg cggggcgtga ggcgctgcgg
     2941 acctaacgga cgctcgccac gacgccatcc cagagcgcct cgtagcgctt cgcgatctgc
     3001 gtgatctcca tatcgcgcat gacgcgacgc cggagcgtgt cgggcgcgtc gggatccacg
     3061 agctcctcga gctcgtcgtc acctacgttg tagtgtgtct gcgagatgcg tgcccgcacc
     3121 acgccgtgaa ggccctcgcc gagcaggctg agatccacca tgtccgaact gagcccgggc
     3181 cccgtaccct cgaccacggc caggagccgg tcggccaagg cggccgtgtc acccgccggg
     3241 tagaaggtca cgctgggatc gcctgcggcc atgcgctgat ggggctcgat ggccgtcgcg
     3301 atgaccggtg ctcccgcggc cgccgcctcc aggagctccc aagggaacaa ggtctcatgg
     3361 ctcggcaaca ccacgacatc cagtgcgtga tagatgcgac tccggtcgat gcggaggccc
     3421 aagaagcgcg tgttgcccag gattcccgac tgatgggcat agtgctccat ggccgccgag
     3481 tcacggccgg agccgacgat gacgaactcg accaaaggca cgcggcgggc cacccgcgcg
     3541 gcggcttcga ggaagtccgc ccagcccgac cgccggtcgt caaactggcc cacgagtccc
     3601 aggtggaggg tatacgggtt cagccccagt tccgccatca gcatctcggc cacggcagaa
     3661 ggcacaaact cgcgcttgtc caggccaccg gggatctgag tcgcacgatt tcgcagtcca
     3721 cgaggtagcg catcgagact caaggcggcc agggcagaag cgcgccagat ctgccggcgg
     3781 ttctgccagt gatcgggcac gggagacagc accgacatcg tccacgggac tcggtagcgc
     3841 cgcgccaacg cctcggccca cgcagcggcc gcgaaaccat gcgcgtggac gagctggggg
     3901 gcgaggtcac ccagctccgc caccgcctgg gccatggcac gccgatcatg gcggcggcgg
     3961 cctgtgagac gaacggccgc cgtgcgcgcc ttcagacgag ccgtacgacg cagcagggcc
     4021 cgattggtgt cagagatcac ggcctgtgac cggcccagcc ccgaccggcg gaccaccgcc
     4081 tcgcagagct cggtgacaaa ctgctgatca cgcaggctct cgccaaatgc caaatgcagg
     4141 atgctcggaa cggcactcac acccatgcca tgcgcaaacg gccccacagg gctcgccagg
     4201 cccattgtag gagaacccgg gcgcaggggg ggccggactc tcagcatggg gctcagaggg
     4261 ccgcccaggc ggccctctgc agccatctcg ccctagagcg gcaggtcgat ggggcgtctc
     4321 tccgcgatgg accgatacgc ggcctccgcc aactccaccg ccgcccgccc gtcgctaccg
     4381 gggacggcga cgggtgtatc actgagaacc gcctccacaa agagccgcac ctcatgttgc
     4441 acggccggct cgacctcaat gtcgccgatc ttctgggcct tgacctcgtc cccaccctcg
     4501 acgccgtaac ggatgtaggc atcgctcgag aacccgttct cgatgaagaa cgtgcccttc
     4561 gagcactgca ctcgccccgt gtaggcaccc atccccgcgt ggcacaagct actcgagtga
     4621 agccagccca ccgcgccgct cttgaacgtg atgctggcga agaccaagtc ctcgtagtcc
     4681 tgttcgtcgc agatgtagtt gccgccgacc gcatagacgc gggcggcgtc gccggccagg
     4741 cagcgcatga agtccaactc gtgagcgttg atctccatca ggttcccgcc cgcctcggct
     4801 agccggagac gccacggcac gccgtagtcc ccgccccagc ggccgccgat ccgctcgatg
     4861 ctaatggcca acacgtcacc gtacgcgccg gacccgatct ggtcccggag ccaatggaac
     4921 gccgggatgt accggaggac ctgcgccacc atgagctgag tgccggcccg gtcggcggcc
     4981 gcgatcatgg cgttgcagtc ggccacggtg ggtgccatcg gcttctcgca cagcacatgc
     5041 ttgcccgacg caacggcgca ctcgaccatg ggccggtgca tgaaaggcgg ggaggcaacg
     5101 atcaccgcat cgacgcctgc ctcggcgacc agcctcccat agtccgtgta gacatccgcg
     5161 gcaaacttgt cgcccgcctc acgcgcccgc gcctcgtcgg tatcgcacac ggcggcgatg
     5221 gtcgagttcg gcagttcgtg acagggcgtg gccagggaca aacccatgcc accgcacccg
     5281 agcagtccaa ccttcagctg cgccatgact ccgatcgatc tcccgtctca cttggtgtgt
     5341 ggagccgcag tgtgttcggg accacgaacg aacgcccctc cgcgctccat cacggctcgc
     5401 gccagcgcag accaggtgtc tttccctcat cgacggcgcg ccagaacgcg tccttcacat
     5461 ccgcgtacag gccctggatg ccgttggcgt gcttgtaccg gtcgatctcg atccaggcgt
     5521 cgggccgcgg gattgggcgg gccgccttca tccgctctgc gtagtcgtgg atggcgtga
//
LOCUS       DAOSPL010000075         5579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_14689_length_5579_cov_2.753802, whole genome shotgun sequence.
ACCESSION   DAOSPL010000075 DAOSPL010000000
VERSION     DAOSPL010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14689_length_5579_cov_2.753802"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1595)
                     /locus_tag="PLQ54_02305"
     CDS             complement(<1..1595)
                     /locus_tag="PLQ54_02305"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOS92120.1"
                     /translation="MQPAPNEMARAAEWAAALRTGAGAVQGYPIGGTLGGEPLERLVA
                     AWGHYHARAETEKGYAVETTVHRDPATGLSIGTKVHVHADFPAVEWVVRLTNEGDTDT
                     GIFEGIQALEAALPLAGGDPCRVVYSLGSMCSRDDFRLTERHLRPRSHLRLTAGWGRS
                     SSEVLPFFNVDFGGNGIIIGIGWTGEWAAQFSRDEPGGLSIRVGMAHTHLKLFPGEEI
                     RTPRVLLLFWEGEPIRGHNALRRFILKHHRPRPNGQPLVAPLCNGNWGGTPADVHLDN
                     ISQIAAHDLPFEYYWIDAEWFGKPGPWPRGAGDWTPRADIYPQGLRPIGDAAHAAGLK
                     FLLWVEPERVAPGTPWARDHTEWLLEVPEGRAVTWTDYGDWMSTEDWVRAESSRNQLN
                     AGDRLLNLGNPDALQFLTDFLSDLINRFGIDCFRQDSNISQLLYWQNADAPDRQGMAE
                     IRYVEGQYALWDALLERHPGLIIDNCASGGRKIDLESLDRATPLWRTDFCDLSPGRVA
                     AQCHTWGLLHWIPLNSTSVGYLDS"
     gene            complement(1792..3453)
                     /locus_tag="PLQ54_02310"
     CDS             complement(1792..3453)
                     /locus_tag="PLQ54_02310"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HOS92121.1"
                     /translation="MARMLPTRLARDTRSAAERRFYEALQHLDDEFTVVHSWNWEGLG
                     GKPMGEADFVLLHPRLPMLVVEVKGGGVEHSGQGWFSIDLRGGCHDIGDPVAQVRDSQ
                     GALVRFLRARARISLPRVGYALAFPDLENWGAAVPPGIDRDCILTRPDLHRGAIGASI
                     GRALKHWARREGPGSRCLGSAGVRCVEEALYPTIRALPSLRATIDEVRREVLQATDDQ
                     WRILEAAQGNRRLFVRGCVGSGKTVLAQEHARRMASQGLHVQALCFNQALARHLAAAL
                     AAAGPGTVSVNTFWTWARDVCAAAGDPLPREFDDAITRKVMPQKLRAALGSATKAPWD
                     ALVVDEGQDFWDEWWAVIDETVVERWGTRLCVFFDSEQTLFVDTASRFLARQSPVLEL
                     TRNCRNPAPVATMAYERAGVRPMPLCTVGGPEPLVSRCASETEARGRLLRIIRDVVAE
                     LGDEAEVGSVVLLSLHTYENSLARRIAGSLPVGWTLAKWTGASPECGPKTIPVCTIQA
                     FKGLEADIVIVELDVDSPHCTAATQLVGLTRSTGMLYVVEYPCAG"
     gene            complement(3458..>5579)
                     /locus_tag="PLQ54_02315"
     CDS             complement(3458..>5579)
                     /locus_tag="PLQ54_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOS92122.1"
                     /translation="QRIRVARCDAETKRARRGNNASSANVVLTNSSMLNWILAGANRN
                     WKELLGGLRFVVVDEVHTARGVVGSNLACILRRLRRLVDHYSLHRCNPQFVLCSATIG
                     NPVELARQLIGDGAPVAAVERDTCGRGRRVFASWTPRAATHGGRRVTVVPDLVEALLD
                     RKDGPVQTVVFHRSRPEANIVARQCKDRLERAGRDDLAGALSVYISNRLGSVKEEELA
                     RLRTGDCPAVFATSALELGVDIGALSAAIIVGMPHSRAGFRQMAGRVGRVSSDSREAL
                     ILYVPRNNPLDDWFAEAEHFEQCLVRARPDDVVMDPDNSVVATQHIAAAAKELPLDLI
                     DDARFFGELRTREAFDSLRANGWIADPGADGKYYWQARDEDPHKRINVLASRGNAQVD
                     IKRGGGQLVGRTDNWSALWMLFPGAIYDDGVRDTYVVESLFLGPMDVGSGGSSADPPT
                     AWVRPARPEEERSYTVAEISSDLTILDESAQSSVSGGRLAVSHGTVAVRAGLTGRYWV
                     CERGEPGWSAAASRKLMNIRSWPQAIDREGGRVEYKELAFETKALWFEVPAHILAAMP
                     TEDVADDVAWERQLGWIHGAEHLVCKMTAVLPGFCGDDVAGLSVYGHPQLSGPGIFLY
                     EDCPGGSGLVDRLMEEGALERVFTAALDAVAACDCADGCPRCVLDRLCGNANDSLSKQ
                     GAEAVLRGLLDEMRHKPARRGRRP"
BASE COUNT          913 a         1973 c         1799 g          894 t
ORIGIN      
        1 gagtccaggt agcccaccga tgtgctgttc agcgggatcc agtgcagcaa gccccaggtg
       61 tgacattggg ccgccacccg cccggggctc aggtcacaga agtccgtgcg ccacaagggc
      121 gtcgcgcggt ccagcgactc caggtcgatc ttccggccgc cgctggcgca gttgtcgatg
      181 atcagccccg ggtgccgctc gagcagcgcg tcccagagcg cgtactgccc ttcgacgtag
      241 cggatctcgg ccatcccctg gcggtcgggg gcgtccgcgt tctgccagta cagaagctgc
      301 gagatgttcg aatcctggcg gaagcagtcg atgccaaacc ggttgatgag gtccgatagg
      361 aagtcagtga ggaactggag agcatcgggg ttacccaggt tcagcagccg gtcgcccgca
      421 ttgagctggt tccgcgagct ctccgcccgc acccagtcct cagtggacat ccagtcgccg
      481 tagtccgtcc aggtcacggc ccgcccctcg ggcacctcga gcagccactc ggtgtgatcc
      541 cgggcccacg gcgtgccggg cgccacccgc tccggctcga cccacaggag gaacttgagc
      601 ccagcggcgt gcgccgcgtc tccgatgggc cgaaggccct gcgggtagat atcggcccgc
      661 ggcgtccagt cgcccgctcc gcgtggccat ggccccggct tgccgaacca ctcggcatcg
      721 atccaatagt actcgaacgg cagatcgtgc gccgcgatct ggctgatgtt gtcgaggtgt
      781 acgtcggccg gcgtgccgcc ccagttgccg ttgcacagag gggcgaccag cggctgcccg
      841 ttggggcgcg gacggtggtg cttcaggatg aaccgccgca gcgcgttatg cccgcggatc
      901 ggttcgccct cccagaacag caggagcacc cgcggcgtac ggatttcctc gccggggaac
      961 agcttgaggt gcgtgtgggc cattccgact cggatagaga gccccccggg ttcatcgcgg
     1021 gagaactgcg cggcccattc tcccgtccac ccgatgccga tgatgattcc gttccccccg
     1081 aagtcgacgt tgaagaaggg gagcacctcg ctggacgaac gtccccagcc tgccgtcaac
     1141 ctcagatggc tgcgaggacg cagatgccgc tccgtcagtc ggaagtcgtc ccgcgagcac
     1201 atggacccca gcgaatagac cacccggcac gggtcgccgc cagccagcgg cagggcggct
     1261 tccagggcct ggatgccctc gaagatcccc gtgtccgtgt cgccctcgtt ggtgagccgc
     1321 acgacccact cgacggctgg gaagtcggcg tggacgtgga ccttagtgcc aatgctcagt
     1381 cccgtcgccg ggtcgcggtg caccgtggtc tcaacggcgt agcccttctc ggtctcggca
     1441 cgcgcgtggt agtgacccca ggcggcgacg aggcgttcga gcggctcgcc gcccagcgtc
     1501 ccgccgatgg ggtagccctg gaccgcgccg gctcccgtcc gcagagctgc ggcccattcg
     1561 gcggcacgtg ccatctcatt gggtgcaggt tgcatggcgc tctcggtctc caaagcagcg
     1621 tgggactggc ccgggccggg cttctccatc gggggggctt cggcctgcgg cattccatgc
     1681 catcggtggc ccgcactgcc ccccaacccc gtcagccttg agggacgcgc accggggcgc
     1741 acctcgaggg gcgggtgtca caggcagtcc ctcgcggccg cgcgacccac actatccggc
     1801 acacgggtac tcaaccacgt agagcatgcc ggtagagcga gtcaggccga caagctgcgt
     1861 ggccgcggtg cagtgcggac tgtcgacgtc cagctcgacg atcacaatat ccgcttcgag
     1921 acccttgaag gcctggatgg tgcagacggg gatcgtcttg gggccgcatt ccggcgaggc
     1981 accggtccac ttggccagcg tccatcctac gggcagcgac ccggcgatcc gtcgggccag
     2041 tgagttctcg taggtgtgca gcgagagcag cacgacggac ccgacttcgg cttcgtcgcc
     2101 cagctcggcc accacatcgc ggatgatgcg cagaagtcga ccccgcgctt cggtctcgct
     2161 ggcacagcgc gagaccagcg gctctgggcc cccgacggta cagagcggca tggggcgcac
     2221 gcccgcccgc tcgtacgcca tcgtcgctac gggagctgga ttccggcagt tccgagtcag
     2281 ctcgagcacc ggagactgcc tggcgaggaa ccgcgaggcg gtatcgacga acagggtctg
     2341 ctcgctatcg aagaacacac acaggcgagt cccccagcgc tccacaacgg tttcgtcgat
     2401 caccgcccac cactcgtccc agaagtcttg accctcatca acgaccagcg cgtcccatgg
     2461 cgccttggtg gccgaaccta gcgcggcacg gagcttctga ggcatcacct tgcgtgtgat
     2521 cgcgtcatcg aactcccgag ggagaggatc gcccgctgcg gcgcagacat ccctcgccca
     2581 cgtccagaag gtgttcacgg atacggtacc cggcccggcg gccgctagcg cagcggcgag
     2641 gtgacgcgcc agcgcttggt taaagcacaa cgcctgcaca tggagtccct gagacgccat
     2701 gcgccgcgcg tgctcctgag cgagcactgt cttgcctgag cccacacaac cgcgtacgaa
     2761 gagccgtcgg ttgccctgcg ccgcctccag aatccgccac tggtcatcgg tggcctgcag
     2821 gacctctcga cgcacctcgt cgatggtggc gcggagtgag ggcaaggccc ggatggtcgg
     2881 gtacagcgcc tcctcgacgc accttacgcc cgccgagccg aggcaacggg agcccggtcc
     2941 ttcgcgccgg gcccagtgct tgagcgcgcg gccgatggat gccccgatgg ccccgcgatg
     3001 gagatccggc cgcgtcagta tgcagtcgcg gtcgatccct ggcgggactg ccgcacccca
     3061 gttctcgagg tctggaaacg ccagggcgta gccgacacgg ggcaacgaga tcctcgcgcg
     3121 agcacgcagg aaccgaacca gagctccctg ggagtccctg acctgtgcca cggggtctcc
     3181 gatgtcatgg cagccgcccc tgaggtcgat cgagaaccac ccttgaccgg agtgctcgac
     3241 cccaccccct ttgacctcga cgacgagcat cggcaggcgt ggatgcagga ggacgaagtc
     3301 ggcttcgccc atgggcttgc ctccgagacc ctcccagttc cacgagtgga cgacggtgaa
     3361 ctcgtcgtcc aggtgctgca gtgcctcgta gaagcgccgc tccgccgcgc tcctcgtgtc
     3421 gcgggccagt ctggtgggga gcatccgtgc catgggccta cggccgcctc cctcttcggg
     3481 ccggcttgtg ccgcatctcg tcgagaaggc cgcgcagcac cgcctcggcg ccctgcttgc
     3541 tgagcgagtc gttggcgttg ccacacagcc ggtccagaac gcaccgcggg cacccatcgg
     3601 cgcagtcgca tgccgctacc gcgtcgagcg ccgccgtgaa gacgcgttcc agggccccct
     3661 cctccataag gcggtcaacg agcccggacc cgccggggca gtcctcatag aggaagatgc
     3721 ccgggccgga caactgaggg tgaccgtaga cagacagtcc agcaacatcg tcgccacaga
     3781 acccgggcag caccgccgtc atcttgcaca cgaggtgctc ggccccatgg atccacccga
     3841 gttgtcgctc ccatgcgacg tcgtccgcga cgtcctcggt cggcatggcg gccaggatgt
     3901 gtgctggcac ctcgaaccag agggccttcg tctcgaaggc gagctccttg tactcgacgc
     3961 gcccgccctc ccggtcgatg gcctggggcc aagagcggat gttcatcagc ttcctggatg
     4021 ccgccgcaga ccatcctggc tcgccccgct cgcacaccca gtaacgacct gtgagtcctg
     4081 ccctgaccgc aacggtgccg tggctgacag cgagtcgccc gccagacacc gaggactggg
     4141 cggactcgtc caggatcgtc aggtcagagc tgatctcagc caccgtgtag ctgcgctcct
     4201 cttccggcct ggcagggcgc acccaagcag tagggggatc ggcgctcgac ccacctgacc
     4261 ctacgtccat cgggcccagg aacagagact cgacgacata cgtgtccctg accccatcgt
     4321 cgtagatagc gccggggaag agcatccaca gtgcggacca gttgtcggtc cggcccacga
     4381 gttggccgcc acccctcttg atgtcgacct gcgcattgcc ccgagacgcc aggacgttaa
     4441 tgcgtttgtg cgggtcctcg tcgcgggcct gccagtagta cttgccatcg gcaccggggt
     4501 ccgcgatcca cccgttcgcc cggagggagt cgaacgcttc cctcgtgcga agctccccaa
     4561 agaagcgcgc gtcgtcgatc agatccagcg gaagctcctt cgctgcggcc gcgatatgct
     4621 gggtcgcgac cacgctgttg tctgggtcca tcacgacgtc atctggcctc gctcgcacca
     4681 ggcattgttc gaagtgctcg gcctctgcga accagtcgtc cagcgggttg ttccgcggga
     4741 cgtagagaat gagcgcctcg cgcgagtcgc tgctgacgcg gcccacgcgc ccggccatct
     4801 gccggaatcc tgccctgctg tgcggcatgc ccacgatgat ggctgcagag agggcgccaa
     4861 tgtcgacacc cagctccagc gcggaggtcg cgaacaccgc cgggcagtcg ccggtccgca
     4921 ggcgcgccag ttcctcctcc ttcaccgacc cgagcctgtt ggatatgtac acgctgaggg
     4981 cgccggccag gtcgtcgcga cccgcacgct cgagccgatc cttgcactgg cgagccacga
     5041 tgttcgcctc gggccgagat cggtggaata ccacggtctg cacgggcccg tccttccggt
     5101 ccagcagcgc ctcaacgagg tcaggcacca cggtcacacg ccggccgccg tgggtcgcgg
     5161 cccgaggcgt ccaactggcg aacacgcgtc ttcctcgtcc gcatgtgtcg cgctccaccg
     5221 ccgcaacagg cgccccatcg ccgatcagct gccgcgccag ctcaaccggg ttgccgatgg
     5281 tggccgagca cagaacgaac tgcggattgc accggtgcag ggagtagtgg tcgacgagcc
     5341 tccggagtct acggagtatg cacgccagat tcgacccgac gacgccgcgg gccgtgtgga
     5401 cctcgtcgac aaccacgaag cggagaccgc ccagaagctc cttccagttc ctgttcgcgc
     5461 cggccagaat ccagttgagc atagatgagt tcgtcagcac cacattcgcg ctgctggcgt
     5521 tgttaccccg acgggccctc ttcgtttcgg catcgcaccg ggccaccctg atcctttga
//
LOCUS       DAOSPL010000076         5573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_14714_length_5573_cov_2.855564, whole genome shotgun sequence.
ACCESSION   DAOSPL010000076 DAOSPL010000000
VERSION     DAOSPL010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14714_length_5573_cov_2.855564"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..2877
                     /locus_tag="PLQ54_02320"
     CDS             103..2877
                     /locus_tag="PLQ54_02320"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOS92123.1"
                     /translation="MTATFGHACLGVVCLTVLSAGWAADELVIDDFGYDGVEAVQAVW
                     KPMSAATPVGLRETRSAATSPGLYLPCPFSQDLERAACDRDVTLDLAPYGRFAIDVFV
                     ENADVCRNFSLYFRSGRGWYSAWVGLDAGWQTLTISKGAFGIEGTPAGWDHIDGIRLS
                     VWKGGDKDTYALVDHLVALASDVAIVLATEAITQTPDEASSVRTFAEGMQAMLDAADI
                     PADMVDDAQVAAGGLAGHRVAILPHCPYLAPETLAALTEFAEGGGKLIACYTLAPEIA
                     RLLSVENLTYVRAEHADQFATFVFEPGAVPGVPERVGQGSWNITGVTPGPGARVIAWW
                     EDRAGNRAPQPAIVQSENGLFIAHVLTRDDLDGKATMLTALIGLFVPDVWLRAAENAI
                     ARATKVGPFTDAAALRQFALSNAADTPGEARVSELLGQAEAALGAARASQVADPPAAC
                     QQAAKARQAFRDAYALAHRPRAGEFRAVWEHSGGGALGNWEDSIANLADAGFTAVVPN
                     MWWGGLAHYDSAYLPHSSLFERRGDQIAQCIEAAHKRGIEVHPWKVNWNLLTAPDSFV
                     EQMRREGRLAVSASGETGRWLCASHPDNLQLEIDTMLEVARKYDVDGIHFDYIRYESD
                     EWCYCDGCRERFQRDTGLTVANWPSQCYSGDLRQAYRDWRCEQITRLVRAVSEQVHRE
                     KPYLKVSAAVFSDYPGCRTYVGQDWVKWVREGLLDFVCPMDYSDNDRWFRQTVSRQNA
                     DIGGRIPFYPGIGASAPGLPPDQVLYQAQLAREIGADGFIIFQHAADTAANHIPMLGR
                     SFTTGATHMPHNGPRVVFTLPAEPSPADGAVHVPGAEAAPVSVRLESLGQHRQRATGI
                     VGTLTLESTYGARLGDLAGGPADAGDEVSVQVQPRDGRVRVVFSGELSMADGTSAPFV
                     VRSLPLVFDR"
     gene            2890..3249
                     /locus_tag="PLQ54_02325"
     CDS             2890..3249
                     /locus_tag="PLQ54_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92124.1"
                     /translation="MGAGNVAAVRAVAMALLALGVPMAETDGQPMRTIEYHGIRPSDP
                     GGRDGLRNPERGWRIETLIAEPPGEPAWGPAAHLLGQISAGYSDEWWILDARHYEPHG
                     LTLARPTATSTATSACQ"
     gene            3240..4520
                     /locus_tag="PLQ54_02330"
     CDS             3240..4520
                     /locus_tag="PLQ54_02330"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HOS92125.1"
                     /translation="MPIADEKLQWLQQSFDALRANGLKAVLRFAYERDMGPPTGPTLE
                     DIQRHLAQLKPIIRRNADVIFVMQAGFVGAWGEWHSSAHKLEADHAILAAVVAGVLDA
                     LPPDRMTQVRVPKYKRWVLEQPAWAPFVEVSADTAHNGTPSSRIGFHNDGFLAGETCG
                     GTWTEPPLFSNPGNPEFDYMTRECPYVPVDGELFWSDQGGPIDGLRAAIRLRLHHYSS
                     FSLAHSFSEREGKPYSIDTWMQTPLTLAQAQEAKLPVSDGYFEGADGEPVDRTPFEYI
                     RDHLGYRIELLRARLLEQLSPGGPVRIEVDLVNRGFSAFTNPRPVFVVLLGDGHEPVA
                     SWQSSADPRAWQPFDPADPDFAPITHTVRVDAELPADLPGGTYRLGLWMPDGYDSLRL
                     DPRYAVRVANRDVPWWTTPAGYGVNILGEVPVKP"
     gene            4598..5158
                     /locus_tag="PLQ54_02335"
     CDS             4598..5158
                     /locus_tag="PLQ54_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOS92126.1"
                     /translation="MAPVSDGELVRRCRLGDRCAFRERVQRHRAMVLRVARAITGSRE
                     DGEDVAQEAFVSAYRALGRYDSSRDFDTWLRAITVRHAVSALRARRRRRPNGAAEVAE
                     SGLGGGDEPAAAIEGAELRQRVRRAIGSLPIRQRVAITLFGLDGADLAETARAMGCSV
                     GAVKTHLHRARERLARLLGDYVEGDG"
     gene            5155..>5573
                     /locus_tag="PLQ54_02340"
     CDS             5155..>5573
                     /locus_tag="PLQ54_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92127.1"
                     /translation="MMRCREARALIHRAEDRREPISAELAEHATACASCRALLEASRQ
                     ASALVAQSGGAVSADELDGLTERVMARLAAPGSRRTAPWPRWALAGVAAAALFLAGLA
                     SGRGLWPRTVVVTRDVPRVRVVDRPVTVIVEKPVVRER"
BASE COUNT          822 a         1990 c         1856 g          905 t
ORIGIN      
        1 tgtacgcgcc ttccgcgcac cgcctgagcc tcacgaccgc caatgccccc cccggaggtt
       61 tccgtgccac cgcccgcgaa gcgccctccg gggtgatccg ccatgactgc gaccttcggc
      121 cacgcctgtc tcggcgttgt ctgcctcaca gtcctcagtg ccggctgggc cgccgacgaa
      181 ttggttatcg acgactttgg ctacgatggc gtcgaggcgg tccaggccgt ttggaagccc
      241 atgtccgccg caactccagt cggactccgc gagacacggt cggccgcgac cagcccgggc
      301 ctgtacctgc cctgcccgtt cagccaggat cttgagcggg ccgcctgcga ccgcgatgtg
      361 accctcgacc tggcgcccta cgggcgcttc gccatcgatg tcttcgtaga gaacgctgac
      421 gtgtgccgca actttagcct ctacttccgc tcgggccgtg ggtggtactc ggcctgggtg
      481 gggctcgatg ccggatggca gacactcaca atctccaagg gcgcattcgg catcgagggc
      541 acgccggctg gctgggatca catcgacggt atccggctct ccgtctggaa gggtggcgac
      601 aaggatacct acgccctcgt cgaccacctc gtcgccctgg cttccgatgt cgccatcgtg
      661 ctcgccaccg aggccatcac ccagacgccc gatgaggcct cttctgtccg gactttcgcc
      721 gagggcatgc aggccatgct cgacgcagcg gacattccag ccgacatggt ggacgacgct
      781 caggtggccg ccggcggcct ggccggacat cgcgtggcca tcctccccca ttgcccctat
      841 ctcgcgccgg agaccctggc cgcattgacc gagttcgccg agggcggtgg gaagctgatc
      901 gcctgttaca ccttggcgcc cgagatcgcc cgactgctgt cggtcgagaa cctcacctat
      961 gtgcgggccg agcatgccga ccagttcgcc acattcgtct tcgagccagg agccgttccg
     1021 ggcgtgccgg agcgcgtcgg tcagggctcc tggaatatca ccggcgtcac gcccggtccg
     1081 ggcgcgcgtg tcatcgcgtg gtgggaggac cgcgccggca atcgtgctcc gcagcccgcc
     1141 atcgtgcagt cggagaacgg gctgttcatc gctcacgtgt tgacccgcga cgatctggac
     1201 ggcaaggcta ccatgctcac tgccctcatc gggctcttcg ttccagacgt gtggctccgc
     1261 gcggccgaga acgccatcgc acgcgccacc aaggtcggac cgttcactga tgccgccgcg
     1321 ctgcgccagt tcgctctcag caatgctgcc gacaccccgg gcgaggcgcg cgtcagcgaa
     1381 ctcctgggcc aggcggaggc cgccttgggg gccgccaggg cctcgcaggt tgccgatcca
     1441 ccggccgcct gccagcaggc agccaaggct cgccaagcct tccgcgacgc ctacgccctg
     1501 gcccatcggc ctcgcgccgg cgagttccgc gccgtgtggg agcactcggg cggaggtgca
     1561 ctgggcaact gggaagactc catcgccaac ttggccgatg ccggcttcac cgccgtggtg
     1621 ccgaacatgt ggtggggtgg gctggcccac tacgacagcg cctacttgcc ccactcaagc
     1681 ctgttcgagc gccggggaga ccagatcgcc cagtgcatcg aggccgccca caagcgcggg
     1741 atcgaagtcc atccctggaa ggtcaactgg aacctcttga ccgcccccga cagcttcgtg
     1801 gaacagatgc gccgcgaggg ccgccttgca gtctcggcct cgggggagac cggtcggtgg
     1861 ctctgtgcgt cgcatcccga caacctccag ctcgagatcg acaccatgct cgaggtcgcc
     1921 cggaagtacg atgtcgatgg catccacttc gactacatcc ggtacgagag tgacgagtgg
     1981 tgctactgcg acggctgtcg cgagcgcttc cagcgcgata ccggactcac ggtggccaac
     2041 tggcccagcc agtgttactc cggtgacctg cggcaggcct atcgcgattg gcgctgcgag
     2101 cagatcaccc gcctggtccg cgcggtgtcc gagcaggttc accgggagaa gccatacctc
     2161 aaagtctcgg cagccgtctt ctccgactac cccggatgcc gaacctatgt gggccaggac
     2221 tgggtgaagt gggtgcgcga ggggcttctc gacttcgtct gccccatgga ctactcggac
     2281 aacgaccgct ggttccgcca gaccgtctcc cggcagaacg ccgacatcgg cggccgtatc
     2341 ccgttctacc cgggcatcgg cgcctcggcc cccggccttc cccccgacca ggtcctctat
     2401 caggcccaac tcgcccgtga gatcggagcc gacggcttca tcatcttcca gcacgccgcc
     2461 gacacggcgg cgaaccacat cccgatgctc ggacggagtt tcaccaccgg agccacgcac
     2521 atgccgcaca acggcccccg ggtggtgttc accttgccgg ccgagcccag ccccgccgac
     2581 ggtgccgtcc acgtgcctgg agccgaggcc gctccggtgt ccgtgaggct cgagtccctg
     2641 ggccagcacc gccaacgggc caccggcatc gtcggtacgc tcaccctcga gagcacctac
     2701 ggagccaggc tgggcgatct cgctggcggt ccggccgacg ccggggacga ggtgtccgtc
     2761 caggttcagc cgcgtgatgg gcgcgtccgc gttgtcttct ccggtgaact gagcatggcg
     2821 gatgggacct cggccccctt tgtggtgcgt agcctgcccc tggtgttcga tcggtgaggc
     2881 ggtgacgaca tgggggcggg taacgtggca gcggtgcggg ccgtggcaat ggcgcttctc
     2941 gccctgggag ttccgatggc cgaaaccgat gggcagccga tgcggaccat cgagtaccac
     3001 ggcatccggc cgtccgaccc tggaggccgc gacggcctcc ggaaccccga acgcggatgg
     3061 cgcatcgaga ccctcatcgc ggaacccccg ggagagcccg cctgggggcc ggccgcgcac
     3121 ctcctcggcc agatatcggc cgggtactcg gacgaatggt ggatcctcga cgcccggcac
     3181 tatgagcctc acggcctcac cctggccaga cctactgcta cctcgaccgc tacgtcggcg
     3241 tgccaatagc cgacgagaag ctccagtggc tccagcagag cttcgatgca ttgcgcgcca
     3301 acggcctgaa ggcggtgctg cggttcgcct acgagcgcga catgggcccg ccgaccggcc
     3361 cgacgctcga ggacatccag cggcacctcg cccagctcaa gcccatcatc cgccggaacg
     3421 ccgacgtcat cttcgtcatg caggcgggct tcgtcggagc ctggggcgag tggcacagct
     3481 ccgcccacaa gctcgaggcc gaccacgcca tcctggccgc cgtcgttgct ggtgtgctcg
     3541 atgcgctccc gccggaccgg atgacgcaag tgcgggtccc caagtacaaa cgctgggtgc
     3601 tcgaacagcc cgcgtgggcg cccttcgtcg aggtctctgc cgacacggct cacaacggca
     3661 ccccgtcgtc gcgcatcgga ttccacaacg atggcttcct cgccggcgag acctgcggcg
     3721 gcacgtggac cgagccaccc ctcttctcga accccggcaa cccggagttc gactacatga
     3781 cccgcgagtg cccctatgtt cccgtcgacg gtgagctgtt ctggtccgac cagggcggcc
     3841 cgatcgacgg gctgcgtgcg gccattcggt tgaggctgca ccactacagc agcttcagtc
     3901 tggcccactc gttctccgag cgagagggca agccgtactc gatcgatacg tggatgcaga
     3961 cgcccctcac gctggcgcag gcgcaggagg cgaagctgcc cgtctccgat ggctacttcg
     4021 agggcgccga tggcgagccc gtagaccgta cgcccttcga gtacatccgc gatcatctcg
     4081 gctaccgcat cgagctcctg cgcgcccggc tgctcgagca gctcagccct ggcggacccg
     4141 tccgaatcga ggtcgacctc gtgaaccggg gcttcagtgc cttcaccaac ccgcgcccgg
     4201 tgttcgtggt gctcctcggc gacggccacg agccggtggc ttcgtggcag tcgtcggccg
     4261 acccgcgcgc atggcagcca ttcgaccccg ccgatcccga cttcgcgccc atcacgcaca
     4321 ccgtccgggt cgacgccgag ttgcccgccg acctgcccgg tggaacgtac cgcctgggcc
     4381 tctggatgcc tgacggttac gacagcctcc ggctcgatcc gcgctacgcc gtccgcgtcg
     4441 ccaaccgcga tgtgccgtgg tggaccaccc ccgccggata cggcgtcaac atcctcggcg
     4501 aggtgcccgt caagccctga gcgaggtcct gtcatcctcg caacgcgccg gggcgtatac
     4561 gcctgtgtgt gagcactcgg atggggccgg atccggcatg gctcctgtga gcgacggcga
     4621 gctggtgcgc cgctgccggc tgggcgatcg gtgcgccttc cgcgagcggg tgcagcggca
     4681 ccgcgcgatg gtgctccgcg tagcccgggc catcacgggc agtcgtgaag atggcgagga
     4741 cgtggcgcag gaagcctttg tcagtgccta ccgcgcgctg gggcgctacg actcctcgcg
     4801 cgacttcgac acatggctgc gggccatcac ggtgcgccac gccgtgtcgg cgctgagggc
     4861 ccggcggagg cgccggccca acggtgcggc cgaggtggcg gagtcgggcc tcggcggagg
     4921 cgacgagccc gccgcggcca tcgagggcgc tgagctccgc cagcgcgtgc ggcgggccat
     4981 cggctcactg ccgatccggc agcgtgtggc catcactttg ttcggtctcg atggcgcgga
     5041 tctggccgag accgcccggg ccatgggttg ctccgtgggc gcggtgaaga ctcacctgca
     5101 ccgtgcgcgg gagcggctgg cccgacttct gggcgactat gtggaggggg atggatgatg
     5161 aggtgccggg aagcgagagc gctgatccat agggccgagg atcgccgaga gcccatctct
     5221 gcggagcttg cagagcacgc gacggcctgc gcctcctgcc gagcgttgct tgaggcatct
     5281 cgacaggcct cggccctcgt ggcccagtcg ggtggcgcag tgtcggctga cgaactcgac
     5341 ggcctcacgg agcgggtgat ggctcgactg gccgctcccg gctccaggcg gacagccccg
     5401 tggccgcgct gggccctggc cggtgtcgcc gccgcggcgc tgttcctggc gggactcgcc
     5461 tcgggccgtg ggttgtggcc gcgcacggtg gtggtcaccc gtgacgttcc gcgggtgcga
     5521 gtggtcgatc gcccggtcac cgtcatcgtt gagaagcccg tggtgcgcga gcg
//
LOCUS       DAOSPL010000077         5565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_14736_length_5565_cov_3.856624, whole genome shotgun sequence.
ACCESSION   DAOSPL010000077 DAOSPL010000000
VERSION     DAOSPL010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14736_length_5565_cov_3.856624"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PLQ54_02345"
     CDS             <1..655
                     /locus_tag="PLQ54_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOS92128.1"
                     /translation="DDACSQCQGTGHLFERCRACGGTGQAPGGAGVFGMIAACGECRG
                     EGEVPRAKCDACRGSGRVHRSRQLEVKVPAGIDNGQRIRLAGEGLAGVGGGPRGDLHL
                     VVKVTPNGFFERRGNDLVCEVPVTFAEAALGAEITVPTKDGTATLKVPPGTQSGQILR
                     LAGMGVPRLRDKGAGDQLVRIKVTVPKKLSRQQRELIETLAASSHEDPRAKLNQYRF"
     gene            676..1029
                     /locus_tag="PLQ54_02350"
     CDS             676..1029
                     /locus_tag="PLQ54_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOS92129.1"
                     /translation="MSGRRSDEPVYMIGVAAKLVGCHAQTLRMYERLGLVSPQRTGTN
                     LRLYSDDDIERLRQIQRLTEVGVNLAGVEMILDLLQKMNRMREEMSAEVARIRAEAEA
                     QITQMRRVLEEPSEQ"
     gene            1151..3610
                     /locus_tag="PLQ54_02355"
     CDS             1151..3610
                     /locus_tag="PLQ54_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS92130.1"
                     /translation="MNAERFTEKAKEVVVAAQDIVQRFGHTMIDVEHLLLAALEQNDG
                     LAVKVIERIAGSDQAIIDLRRDLEREIAKTPRVEYHDASAQAQIYMSPGFRRVMGSAE
                     QEAQRLGDSFVGVEHLLIGILDESAAPAAKLLLARGVDREKIYAALKEIRGSHRVTDA
                     HAEDRYQTLKRFGRDLTALAEANELDPVVGRDEEVRRVIQVLSRRTKNNPVLIGEPGV
                     GKTAIVEGLAQKIVSKDVPEMLRGKRVVALDMGALVAGTKFRGEFEDRLKAVIEEIRA
                     ARGEIVLFIDELHTVVGAGAAEGAIDASNMLKPALARGELQCVGATTLDEYRKYIEKD
                     AALERRFQPVFVEEPSVEQALAILQGLRPRYEQHHGIRFSDGALAAAAKLSHRYLTER
                     RLPDKAIDLMDEAGSKLRMEIESRPPELRQLEERLEAVTREGQAAVQDQRFERAAQLR
                     DEAEALQAELRNARAAWERETGIDRVVDEHDIADIVSRWTGIPVSRMFEGESDRLLRM
                     EEHLHQRVKGQDRAVAAVSECIRRSRAGLADPRRPMGSFIFLGPTGVGKTELARALAQ
                     FLFDDEAAMVRIDMSEYMEKHSVSRLIGAPPGYVGYEEGGQLTEAVRRRPYRVVLFDE
                     IEKAHPDVFNVLLQLLEDGRLTDSAGRTVDFRNTVVILTSNLGTQWLTPPDPATAADE
                     AERQFARQRERVLEELRRTLRPELLNRIDEIIVFAPLNASQLDEIVELMLDRVRRQLA
                     EREIHLEVTPDARRLLATRGYDPTYGARPLRRTIQQLVENPISSWILAGRVQAGMTVV
                     ADRGPDDTIELRAVGSPGPSQ"
     gene            3674..4264
                     /gene="grpE"
                     /locus_tag="PLQ54_02360"
     CDS             3674..4264
                     /gene="grpE"
                     /locus_tag="PLQ54_02360"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOS92131.1"
                     /translation="MKIPIEDPHQDDNATCDESNGSTEAAGDESAGPPVQDEVPDAVA
                     ALEAELAQERDRHLRALADFDNYRKRARRDLADAQAAARFDLIQKLLPTLDNLERALA
                     AHGSRASALRQGIQMVYDQLRDVLVAEGLCAVETVGTPFDPNVHEGVLAMPSNEVPPG
                     HVVQELERGYVFADRVVRPAKVVVCAPPDPEASGET"
     gene            4428..4730
                     /locus_tag="PLQ54_02365"
                     /pseudo
     CDS             4428..4730
                     /locus_tag="PLQ54_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013860659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
     gene            4915..>5565
                     /locus_tag="PLQ54_02370"
     CDS             4915..>5565
                     /locus_tag="PLQ54_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOS92132.1"
                     /translation="MPKELLFSEDARRALERGANMVANAVSITLGPKGRNVVLDKKFG
                     SPTITKDGVTVAKEIELEDIFENMGAQLVREVASKTNDVAGDGTTTATVLAQAMIQGG
                     LKNVAAGSNPMLLKKGMDAAVEAVVAAIAKGATPVADRAAIKHVAAIAGNDPEIGRLI
                     ADAMEKVGREGVITVEESKSSDTRVELTDGMQFDKGYLSPYFVTDPEAMEAVFEKPL"
BASE COUNT         1047 a         1850 c         1850 g          818 t
ORIGIN      
        1 cgatgacgcg tgctcccagt gccaggggac cggtcatctg ttcgagcggt gccgggcgtg
       61 cggcggcact gggcaggccc cgggaggtgc cggggtgttc ggtatgatcg ccgcctgtgg
      121 cgagtgtcgg ggcgagggcg aagtccctcg cgcgaagtgt gacgcctgcc ggggctctgg
      181 tcgggtccac cggagtcgcc agctcgaggt caaggtgccc gccggcatcg acaacgggca
      241 gcgcatacgg ctagccggcg agggactcgc cggtgtggga ggaggccccc ggggcgacct
      301 gcatctggtc gtcaaagtaa cacccaacgg cttcttcgaa cgccgtggca atgacctggt
      361 ctgcgaagtg ccggtcacct tcgccgaggc ggccctgggc gcggagatca ccgttcccac
      421 caaagacgga acggccacgc tcaaagtccc gcccggcacg cagagcgggc agatcctccg
      481 gttggcaggc atgggcgtgc cccggttgcg tgacaagggc gccggcgacc aactggtacg
      541 aatcaaagtc accgtgccca agaaactctc gcgccagcag cgcgagctga tcgagactct
      601 ggccgcgtca tcgcatgagg acccgcgagc caagctcaac cagtaccgct tctgacccaa
      661 gggggtgcga gcgcgatgtc cggacggcga agcgacgagc ccgtctacat gattggtgtg
      721 gccgccaagc tggtcgggtg tcatgcgcag acactccgga tgtacgagcg cctcgggctg
      781 gtgtcccccc agcgaacggg caccaacttg cggctctact cggatgatga catcgagcgt
      841 ctgcgccaga tccagcggct caccgaagtc ggcgtgaacc tcgccggcgt cgagatgatc
      901 ctcgacctgc tacaaaagat gaaccgcatg cgcgaagaga tgtcggccga ggtggctcgc
      961 atccgggcgg aggccgaggc gcagatcacg cagatgcgac gggtgctcga agaaccatcg
     1021 gaacagtaag ggttcccgcc atcgcgccgg ccccatcacc gggcggtacg tgcccagggc
     1081 ttcgcacgcg gcccggccct tccatcgggc cgaagaccct gatccaccgc gagaggaggc
     1141 gacatgacgc atgaatgctg aacgcttcac tgagaaggcc aaagaggtcg tcgtcgccgc
     1201 acaggatatc gtgcagcgct ttggccatac catgatcgac gtcgagcacc tgctcttggc
     1261 cgcactcgaa cagaatgatg ggctcgccgt gaaggtgatc gagcggatcg ccgggtcgga
     1321 ccaggccatc attgacctgc ggcgcgatct cgagcgggag atcgccaaga cgccgcgcgt
     1381 cgagtaccac gacgcctctg ctcaggcgca gatctacatg tcgccgggct ttcgccgggt
     1441 catgggctcg gccgagcagg aggcacagcg gctcggcgac tccttcgtgg gggtcgagca
     1501 cctgctgatc ggcatcctcg atgagagcgc cgcgcccgct gccaagctcc tcctggcgcg
     1561 aggggtcgat cgcgaaaaga tctatgcagc cctcaaggag atccgcggct cgcatcgcgt
     1621 caccgacgca cacgccgaag accggtacca gacactgaaa cggttcggcc gtgatctcac
     1681 tgccctggcc gaagccaacg agctcgaccc ggtggtgggc cgcgacgaag aggtgcgccg
     1741 cgtcatccaa gtcctctcgc gacgaaccaa aaacaacccg gtgctcatcg gcgagcccgg
     1801 tgtgggcaag accgccattg tcgagggtct ggctcagaag atcgtctcaa aagacgtccc
     1861 cgagatgctc cgaggcaagc gcgtcgtcgc tctcgacatg ggagccctgg tcgccgggac
     1921 caagttccgc ggtgagttcg aggaccgcct caaggcagtc atcgaggaga tccgagccgc
     1981 ccggggcgag atcgtgctgt tcatcgacga gctgcacacc gtggtcggcg ccggagccgc
     2041 cgagggggcc atcgatgcct ccaacatgct caagccggct ctggccaggg gcgagctcca
     2101 gtgcgtcggc gcgaccaccc tggacgagta ccgcaagtac atcgagaaag atgccgctct
     2161 cgaacgccga ttccagcccg tgttcgtcga ggaacccagc gtcgaacagg ccctcgccat
     2221 cctccaggga ctacggccgc gctacgagca gcaccatggc atccgcttct ccgatggtgc
     2281 gttggcggcc gctgccaagc tctcccaccg ctacctcacc gaacgccgtc tgcccgacaa
     2341 agccattgac ctcatggatg aagccggctc caagctgcgc atggagatcg agagtcggcc
     2401 gcccgagctg cggcagctcg aggagcgtct cgaagccgtc acgcgggagg ggcaagctgc
     2461 tgttcaggac cagcgcttcg agcgcgcggc ccagctccgc gatgaggccg aggcccttca
     2521 ggccgaactc cgcaatgctc gagcggcctg ggagcgagag accggtatcg atcgggtcgt
     2581 cgacgagcac gacatcgccg acatcgtttc gcgctggacc ggcattcccg tgagtcgcat
     2641 gttcgagggc gagtccgacc gcctgctgcg catggaagag cacctccacc agcgggtcaa
     2701 ggggcaggac cgcgcggtgg cggccgtgtc cgaatgcatc cggcgaagcc gcgccggcct
     2761 cgccgatccc cgtcggccca tgggctcctt catcttcctc gggcccacag gcgtcgggaa
     2821 gaccgagctc gcccgagccc tggcccagtt cctgttcgac gacgaggccg ccatggtgcg
     2881 catcgatatg tccgaataca tggagaaaca ctccgtctcc cgcctcatcg gcgcgcctcc
     2941 aggatatgtc ggctacgaag agggcgggca gctcaccgag gccgtgcgac ggcggccgta
     3001 ccgcgtggtg ctcttcgacg agatcgagaa agcccacccg gacgtgttca acgtcctgct
     3061 ccagctgctg gaagatggcc gtctcaccga ctccgccggc cggacagtgg acttccgcaa
     3121 caccgtggtg atcctgacgt cgaacctggg cactcaatgg ctcacaccgc ccgaccccgc
     3181 cacggcagcc gacgaggccg agaggcagtt cgcccggcag cgagagcgtg tcctcgagga
     3241 gctgcggcgc acgctgcgac ccgagctgct caaccgcatc gacgagatca tcgtgttcgc
     3301 gccgctgaac gcgagccagc tcgatgagat cgtcgagctg atgctggacc gcgtccgccg
     3361 gcagcttgcc gagcgagaga tccacctgga ggttacgccc gacgcgcgcc gactgctggc
     3421 cacgcgtggc tacgacccca cctacggtgc gcgtccgctg cgccgcacca tccagcaact
     3481 ggtcgagaat cccatctcat catggattct ggccggccgg gtccaggccg gcatgaccgt
     3541 cgtggccgac cgcgggccag atgacaccat cgagcttcgc gccgtcggca gtcccggacc
     3601 atcccagtag acccggtgtc cccgaaagca ggatcagagc cctgtccccg cttcgggtgg
     3661 tgaggagaac ttcatgaaga taccgatcga ggatccgcac caagacgaca acgccacgtg
     3721 cgacgagtca aacggctcca ccgaggccgc tggcgacgag agtgccggcc cgccggtgca
     3781 ggacgaagtc cccgatgctg tggcggccct cgaggccgaa ctcgcgcagg agcgagaccg
     3841 gcacttgcgt gccctggcag acttcgacaa ctaccgcaag cgggcgcggc gtgatctggc
     3901 cgacgcccag gccgcggcgc gcttcgacct gatccagaag ctgctgccca ccctcgacaa
     3961 cctcgaacgg gccctcgcgg cgcacgggag ccgagcctcg gcactacggc agggcatcca
     4021 gatggtctac gatcagctcc gcgacgtgtt ggtggccgag ggcctctgtg ccgtcgagac
     4081 cgtgggcact ccattcgacc cgaacgttca cgagggagtg ctcgccatgc cctccaacga
     4141 ggttcccccc ggccacgtcg tgcaggaact cgagcgcgga tatgtcttcg ccgatcgcgt
     4201 cgtgcgcccc gccaaggtcg tcgtctgtgc cccgcccgac ccggaggcgt ccggcgagac
     4261 gtaggtcggc cacccagcct gtccgcaatg cgctccgcgc cattttcgcc tggagtgcta
     4321 gcactctctg ccaatgagtg ctagaatgcg cccgtgagat agccagtggc cgactcgtcg
     4381 tgtgagttcg gtcaggtcgc gatcgccctg ccccccttcg ggggcggatg acgaagcgaa
     4441 aggaggtgct gagggtgctc aagcctttgg gagatcgtgt cgtggtgaag gtcgacgaca
     4501 gcccggagct gtccccggga ggcatcgtcc tgccggatgc ggcccaagag aagccgcagc
     4561 gcggcgtggt ggttagtgtg ggggagggca agcggctcga cgacggcaag atcgtggcgt
     4621 tggatgtgaa ggtcggcgac cgcgtgatct tctccaagta tggcggagtc gaagtccgcg
     4681 atggcaacga ggagttcgtg atcctgcgac aggatgacat ctacgccgtg gacacgtcgg
     4741 cgccgaagcc tgtgtcggcc gctgcggcac caaagcccgc tgcgcagaag gcgggggcca
     4801 agaaggctcc ggtcaagaag accccgggga agaagccggc gtcccgaaag tagacggccc
     4861 gcagcgagag cgagttggga ccgcggaagt ccgacaagga ggaacacgaa gagaatgcca
     4921 aaggagctgc tgttcagcga ggacgcgcga cgggctctcg agcgcggcgc caacatggtc
     4981 gccaacgcgg tgagcatcac gctcggaccc aagggccgca acgtcgttct cgacaagaag
     5041 ttcggctccc ccaccatcac caaagatgga gtgacggtgg ccaaggagat cgagctcgaa
     5101 gacatcttcg agaacatggg cgcacagctg gtccgcgaag tcgcgagcaa gaccaatgac
     5161 gtagccggcg acggcaccac cactgccacc gtcctggccc aggccatgat ccagggcggc
     5221 ctcaagaacg tagccgccgg cagcaatccc atgctgctga agaagggcat ggacgcggcc
     5281 gtggaagccg tggtcgccgc catcgccaaa ggggctactc cggtggccga ccgggccgcc
     5341 atcaagcacg tggccgccat cgcgggcaac gatcccgaga tcggccgcct catcgccgat
     5401 gccatggaga aagtcggccg tgagggcgtc atcaccgtcg aggagtccaa gagctccgac
     5461 actcgcgtcg agctcaccga cggcatgcag ttcgacaagg gatacctctc gccgtacttc
     5521 gtgaccgacc ccgaggcaat ggaggccgtc ttcgagaagc cactc
//
LOCUS       DAOSPL010000078         5547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_14790_length_5547_cov_3.370721, whole genome shotgun sequence.
ACCESSION   DAOSPL010000078 DAOSPL010000000
VERSION     DAOSPL010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14790_length_5547_cov_3.370721"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLQ54_02375"
     CDS             <1..229
                     /locus_tag="PLQ54_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015845637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92133.1"
                     /translation="SPEGAPVSATVRGRQVPGWVMVRGSAADVPEGPVMTEEPEEELT
                     LIPYGCTNLRVTEFPVLGRQGRSAEPGGPGR"
     gene            337..2679
                     /locus_tag="PLQ54_02380"
     CDS             337..2679
                     /locus_tag="PLQ54_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92134.1"
                     /translation="MSRNVTPRVALALCVYLLPAAAQTSGYGPIDIVPPTDLPVAVDR
                     AVADKSWEQDDLSLITTELSPATLMLCRDSSVRFFANLDAWGLGGPNRVAVPGIDTVR
                     LMARGDRLDGSVQTEAWLLAWFGGARGWDPFDIPWLLVLQHRPATIALDGNGLVLRFD
                     GPCGYVASMPLYGYAKPPGPARGQGLMAEQSPATFGVNTAAWYPGLPPEVLDRCRWWA
                     QVLRAFPVDCRETFSVDRRNDSVTLRQEMTWLAVADDFGTRPRKFAPLSPSLGLAWLV
                     GGFPMRCSGPITDAKLFTPYGPYVGVEGADTVEVGLDVLKYVHEVEAQQEPDLDRDPI
                     VAQALETIRQRTSKRYQGDTDRIIWDHGGPENYCWQIMADRWFPKALAYMDPATRERA
                     KAQLHYYFANYVLKEDQYKPLGDHLLLVGPGIGTWGGYDDAGKFSSNVLETLWCYAHY
                     TGDWALIRERWPLILKLFVTPMEMRWKTFGRQSIAEMGDEAAPPLYLARMAYRMGDAD
                     TYGYACYCFARELVHHYVKHKGAEYFRQHGPVDSMEPMTGPIYLTNLWGDTVGWRIDG
                     PQYPKETGERQYGNRWVRFSSEDVARFHRDTCGSECRAELDGLLQVESPYRVGRDTAH
                     IAPSMERLRSLLLNEPPEQLAKLVQGERDPGNDADTIAYYRSFLRTSREPRYDRLIPA
                     GGDPTPFVVGIQRVCRPDWPLLVQAVMGSVGEGDATQPGWPVASWALWKPPTTATGMP
                     AADRWPFGQVLPGDGSPPATFRTTDLSWVTQAMSWGRVAE"
     gene            2945..4354
                     /locus_tag="PLQ54_02385"
     CDS             2945..4354
                     /locus_tag="PLQ54_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOS92135.1"
                     /translation="MPALEPMSLAVTGATTSLLSHPCRIGTLAPMCRPRNVILIVVDC
                     LRADHLGCYGYAKPTSPNIDAFAQTATQFDQFFASAVPTQPSFTTMYTGQYSITHGIV
                     SHKGDVELDENAPWLPSILRAHGMTTAAFCCLPRYKHWFIRGFEFAVDSTTRYLSDGY
                     TAELMNSRAIPWLKAHSDERFFMTIHYWDPHTPYMPPERYRIFYDGDPTDPAKPDTLA
                     PLQRQYFSVMWANWFSKLPQPLRDASYIESLYDGEVRHADDGVGRLLQALDESGRADD
                     TLVVLTSDHGEMFYRHDIFFDHHGLYDGNIHCPLLIRWPGVTAGGRRLHGFAQHQDLA
                     PTVLDALGIGAAEAMDGASLVPALSGGEMPPRDFVVAEECTWQKKWAIRTETEKLIVS
                     RAPDFHGRPMRELFDLRADPDELCDIAEAEPERAHALEERLEGWIDAMVAKNGLTGDP
                     LAASDITLGKKWEQWLRNS"
     gene            4336..5502
                     /locus_tag="PLQ54_02390"
     CDS             4336..5502
                     /locus_tag="PLQ54_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS92136.1"
                     /translation="MAPELVRPRDRLLTALDHHEPDRVPLDLGSTHVTGIALAAYRRL
                     ASLLGVDESPPLVVDDIQQIALPHDRLLEQLGVDTRGLFPVTGNNIRVPLVEVGSDWE
                     YRDEWGLVHRMPRDRGLYFTLVESPLRGPAPDARALSRYTWPSAAEPSRIAGLAERAR
                     TCRQAGYCVVLKGFCAGLCEVACRLRGMESFLCDLVSDPAAAGGLLDRLLELKAAFWE
                     MALPVLAPHVDVVLEADDYGTQDSQLVSPHVFRELFKPRLAELCRVIKALAPGAKLLF
                     HSCGSIRPIIPDLIEIGVDILNPIHTRAAGMEPIGLKRDFGDALCFWGGGVDTQHVLP
                     FGTPGEVRDDVRRNIDALAPGGGYVFSTVHNIQADVPPVNVMAMYEALAEAGVY"
BASE COUNT          883 a         1843 c         1858 g          963 t
ORIGIN      
        1 ctcccccgag ggcgcgccgg tgtcggccac ggtgcggggc cggcaggttc caggctgggt
       61 gatggtccgg ggctcggcgg ccgatgtgcc cgagggtcca gtgatgacgg aggagccgga
      121 ggaggagctc acgctcatcc cgtacggctg cacgaacctc cgcgtgactg agttcccggt
      181 gttggggcgc cagggccgaa gcgcggagcc gggcggccca ggacgatgat cggtgccacc
      241 aaccgcggcg gcgcgtatcc tatagaccat gcagtccttt ggccggtggg accagcctca
      301 ccggctggca tcttggccgg atggggagcc ttcgccgtga gccggaacgt tactccgcgc
      361 gttgctcttg ccttgtgcgt gtacctcctg cccgctgcgg cacagacttc gggctacggc
      421 cccatcgaca tcgtgccgcc gaccgatttg ccggtggccg tcgatcgagc ggtggccgac
      481 aagagctggg aacaggacga cctgtcgctg atcacgaccg agctctcgcc ggcgacactg
      541 atgctgtgcc gcgacagctc cgtgcggttt tttgcgaacc tcgacgcgtg gggactgggg
      601 gggccgaatc gggtagccgt tccgggcatc gacacggttc gcctgatggc ccgaggcgat
      661 cggctcgacg ggagcgttca gacagaagcc tggcttctgg catggttcgg cggtgcccgg
      721 gggtgggatc cgttcgacat cccctggctt ctggttctgc agcatcgccc tgcaaccatc
      781 gcgctcgacg gcaacggtct cgtcctgcgc ttcgacggac cgtgcggcta tgtggcgtcg
      841 atgccgctgt acggctatgc caagccaccc gggccggctc ggggccaggg cctgatggcg
      901 gagcaaagcc cggccacgtt cggcgtgaac acagcggcat ggtaccccgg actccctccc
      961 gaggtgctcg accggtgccg gtggtgggct caggtgctcc gagccttccc ggtggactgc
     1021 cgcgagacgt tctcggtcga ccggcggaac gattcggtga ccctccgcca ggagatgacc
     1081 tggctggcag ttgccgatga cttcggcacg agaccacgca agttcgcgcc gctgtcgccg
     1141 agcctgggtc tggcttggct cgtgggcggc ttccccatgc gctgttcggg gccgatcacc
     1201 gacgccaagc tattcacgcc ctacggcccc tatgtgggcg tcgagggagc ggacacggtc
     1261 gaggtagggc tcgatgtgct caagtacgtg cacgaggtcg aggcccagca ggaaccggac
     1321 ctcgatcgag atcccatcgt cgcgcaggcc ctcgagacca tccgccagcg aacctccaag
     1381 cggtaccagg gcgacacgga tcggatcatc tgggaccatg gcggccccga gaactattgc
     1441 tggcagatca tggccgaccg gtggttcccc aaggcgctgg cttacatgga cccggcgacg
     1501 cgcgagcggg ccaaggccca gctccactac tacttcgcca actacgtgct gaaggaagat
     1561 cagtacaagc ccttgggcga tcacctactg ctggtcggac ccggcatcgg cacgtggggc
     1621 ggctatgacg atgcaggcaa gttcagcagc aacgtcctgg agacgctatg gtgctatgcg
     1681 cactacacgg gcgactgggc actgatccgc gagcggtggc cgctgatcct gaagctgttc
     1741 gtcacaccca tggaaatgcg ctggaagacc ttcggccggc agtctatcgc cgagatgggt
     1801 gacgaggcgg ccccgccact ctatctggcg cggatggcct accgcatggg cgacgcggac
     1861 acctatgggt acgcctgcta ctgcttcgct cgcgagctcg tgcaccacta cgtgaagcac
     1921 aaaggcgcgg agtatttccg ccagcacggg cccgtcgatt cgatggaacc gatgacgggc
     1981 cccatctacc tgacgaacct gtggggagat acggtgggct ggcggatcga cggaccccag
     2041 taccccaagg agacgggcga gcggcagtat ggcaaccgct gggtgcggtt ctcgagcgaa
     2101 gatgtggcgc ggttccatcg cgacacttgc ggctccgagt gccgtgcgga gctcgacggg
     2161 ctcctgcagg tcgagtcgcc ctaccgggtc gggcgcgaca cggcacacat cgcgccctcg
     2221 atggagcggc tgcggtcgct actgctcaac gaaccgccgg agcagctcgc caagctggtg
     2281 cagggcgaac gcgatccggg caacgacgcg gataccattg cctactacag gtcgttccta
     2341 cggacgagtc gcgagccgcg atatgaccgg ctgattccgg cgggtgggga cccgacgcca
     2401 tttgtcgttg gcatccagcg ggtgtgtcgg cccgactggc cactgctggt gcaggccgtg
     2461 atgggctcgg tgggagaggg cgacgccacc cagcccggat ggccggtggc gagttgggcg
     2521 ctctggaagc cgcccacgac cgccaccggc atgcctgcgg cggaccgatg gcccttcggt
     2581 caggtgcttc cgggcgacgg cagcccgccg gcaacgtttc ggacgacgga cttgtcgtgg
     2641 gtcacccagg cgatgtcatg ggggagggtg gcggagtagg ggcatgggcg gcggcgtggg
     2701 gccgccagcc gtcgctcggc ctcgttgcgg acggggcgcc gtagggcgaa ctgcgccgat
     2761 cccaacgggg tgagctcgcg atgcgcccga tcctcgtgac ggggcttcgc gtccttcgac
     2821 aggctcagga gagaggcccg atgccctgtg gcgcaccctg gtgcggcctc tgcacaggac
     2881 cgtgtcgggt tctggcggtc catgcccaag ccgaagttgc cgcgcacgtg aagtcgaggc
     2941 caccttgcca gccctggaac cgatgtccct ggcggtgact ggggcgacca catccctctt
     3001 gtcgcacccc tgtcgcattg gtacactcgc ccccatgtgc cggccccgta acgtgatcct
     3061 catcgtcgtc gactgcctcc gggcggatca tctcggctgc tatggttacg ccaagccgac
     3121 gagcccgaac atcgacgcat tcgcgcagac ggcaacgcag ttcgatcagt tcttcgcgtc
     3181 ggccgttccc acacagccct cgttcacgac gatgtacact gggcagtatt cgattactca
     3241 cggcatcgtt agccacaagg gtgacgtgga actcgacgag aacgccccat ggctgccttc
     3301 gatcctccgg gctcacggga tgacgaccgc cgcattctgc tgccttcccc gctacaagca
     3361 ctggttcatt cgcggttttg agttcgccgt ggattcgacc acccgctatc tcagtgatgg
     3421 ctatacagcc gagctaatga actcccgcgc catcccgtgg ctgaaagccc actccgacga
     3481 acgcttcttt atgaccatac actactggga tccgcatacg ccctacatgc cgcccgagcg
     3541 ctaccggatc ttctacgacg gggaccccac cgacccagcg aagcctgaca ccctcgcccc
     3601 gctccagcgc cagtacttct cggtcatgtg ggccaactgg ttctcgaagc tgccccagcc
     3661 gctgcgagac gcttcgtaca tcgagtcact gtacgatggc gaggtgcgac acgccgatga
     3721 cggggtcggg agactccttc aggcgctcga cgagtccgga cgggccgacg acacactggt
     3781 cgtgctcacc tccgaccacg gtgagatgtt ctaccggcat gacattttct tcgaccatca
     3841 cggcctgtac gacggcaaca tccactgccc tctgctcatc cggtggccgg gggtcacagc
     3901 cgggggcagg cggttgcatg gcttcgctca gcatcaggac ctggctccga cggtgctcga
     3961 cgccctgggc atcggcgccg cggaggcgat ggacggcgcg agcctggtgc cggcgctctc
     4021 gggtggtgag atgccgccac gggatttcgt ggtggccgag gagtgcacgt ggcagaagaa
     4081 gtgggcgatc cggactgaga ccgagaagct cattgtgtca cgcgctcccg acttccatgg
     4141 tcgacccatg cgcgagctgt tcgatcttcg cgccgacccc gacgagctgt gcgatatcgc
     4201 cgaagcggag cccgagcgtg cccacgcgct cgaggagcgg ctggagggat ggatcgacgc
     4261 catggtggcc aagaacggcc tgaccgggga tcctctggcc gccagcgaca tcaccctcgg
     4321 caagaagtgg gagcagtggc tccggaactc gtgaggccgc gcgaccgcct gctcacggct
     4381 ctggatcacc acgagcccga ccgcgtgcct ttggacctgg gctccaccca tgtcaccggc
     4441 atcgccctcg ccgcctacag gcggctggcc agtcttctcg gcgtggacga gtcgcctcct
     4501 cttgttgttg acgatattca gcagatcgct ctaccgcacg acaggctcct cgaacagctt
     4561 ggagtagaca cccgtgggct cttcccggtg accggcaaca acatccgcgt gcccttggtg
     4621 gaagtgggtt cggactggga gtaccgggac gagtggggcc tcgtgcaccg tatgcccagg
     4681 gatcgaggcc tctacttcac gctcgtcgag agccctcttc gaggccccgc gcccgacgct
     4741 cgtgcgctct cgcgctatac ctggccatcg gccgccgagc cgtcgcggat cgccggcctc
     4801 gccgaacggg cccgtacttg ccggcaggct ggctactgcg ttgtcctgaa gggcttctgc
     4861 gccgggctgt gtgaggttgc ctgtcggctc cgagggatgg agagcttcct gtgcgacctg
     4921 gttagtgatc ccgccgcggc cggcggcctt ctggatcggc tcctggagct caaggccgcg
     4981 ttctgggaga tggcgctgcc cgttctggcg cctcacgtcg atgtcgtgct cgaagccgac
     5041 gactacggca cgcaggactc gcagctcgtc tcgccccacg tgttccgcga gctcttcaag
     5101 ccccggctgg cggagctgtg tcgggtcatc aaggccctgg cgcccggagc caagctgctg
     5161 ttccactcgt gcggcagcat ccgcccgatc atcccggacc tcatcgagat cggcgtcgac
     5221 atcctcaacc ccatccacac ccgggccgcg gggatggaac ccatcgggct gaagcgcgac
     5281 ttcggcgatg cgctctgctt ctggggcggc ggggtcgaca cccagcacgt gctccccttc
     5341 ggcacgccgg gcgaggtgcg ggatgatgtc cgccgcaaca tcgacgccct ggctcccggc
     5401 ggcggttacg ttttcagcac ggtccacaac atccaggccg acgtgccgcc ggtgaatgtc
     5461 atggccatgt acgaggcact ggccgaggcg ggggtgtact gaccctttcg agccccgaga
     5521 cccgactgga ctcggccgag ctgctgg
//
LOCUS       DAOSPL010000079         5506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_14910_length_5506_cov_3.359567, whole genome shotgun sequence.
ACCESSION   DAOSPL010000079 DAOSPL010000000
VERSION     DAOSPL010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14910_length_5506_cov_3.359567"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2425)
                     /locus_tag="PLQ54_02395"
     CDS             complement(<1..2425)
                     /locus_tag="PLQ54_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92137.1"
                     /translation="MAELAPQEVFSGECLSYPGPWAFHLPSAGIILVSDQELVDLAGD
                     PDKVLNLTTGRDPMNRSLRQICDEAQARGAHTLVVAFDHFFAQYRPGIDHARTLTPDM
                     DEYVQLIARVGRFAKERGLKLELSLLSPLEIGPAYGKQTGEWGRWYHYRKGLRDPRTG
                     AFSVQMWQHLRWVNNKGPIDLEHLGVRVFAFGQRPVGGTSCLAVAEDSIVEISDVARV
                     EAFEGIRSGSVGRRIRVHGTGRTDVGELDHVLVVQQYRSPEMDYFSDSALPYLKALIG
                     RYLDAGVELSGFYSDEMHIQQDWDYFGHHEHGQFALRYVSPGLERRFAERYGPQYADL
                     AKYMVYFTYGQEDAVAGLTATEPIPHVFGPSAEDIHRTALFRSRYYELLQNGVVDLFL
                     AARRDAEERTGRRMYTRAHATWAESPTIDYYRSGARNANSIRYEYDSRFVWSNTVHQA
                     AAACHDYFKWGEFLTGTGNDHPECGWLDRNYYGMALACSTGIVNELPYSYCAHWGSPN
                     EVAWRREHVGRAFGTTGHMTMHTLVQEMQHRDVSVLMLYPLDLVSTEERFGSWMTQYA
                     YANYVTPAKLLEMGRLVDGAVELGGRRFTTLAALFEPFPRPELLDLMSRLAEAGGRAI
                     WSGPPPLLGRDGEPVRARWADLFGVDPVASPDGLGEMAPGGRVTFEGALEGIEAQIIL
                     TDAVVDHIHPVGPREGTQVTARCAGRVVGTVRGTIAGGALAFVGYRPRDDQSASLGYE
                     TRNWFETLNALGAYPPSAADLPANDNTEVLSRTTPYLFCHFPNGTVAVAPHLTRLEED
                     WPGGFARNPE"
     gene            2619..2924
                     /locus_tag="PLQ54_02400"
                     /pseudo
     CDS             2619..2924
                     /locus_tag="PLQ54_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            3039..3203
                     /locus_tag="PLQ54_02405"
     CDS             3039..3203
                     /locus_tag="PLQ54_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92138.1"
                     /translation="MPYYLEIWDPDTYADYGTAGGYVAKRHNDGFNASFCDGHAKWIK
                     NSKHSMWYPG"
     gene            3238..4599
                     /locus_tag="PLQ54_02410"
     CDS             3238..4599
                     /locus_tag="PLQ54_02410"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOS92139.1"
                     /translation="MPLGARTNWASQPWMLVEHNDLEVEWQQATEEGRDLASVEAEFA
                     DLRASQLDTEELRVRAEALLDRVQELPLVAGYAYEEPSDLDGIRAVRPEAALPPKGRP
                     DRATLEDKALGAWCGRASGCLLGKPAEGRRRWQIEKYLKAQSRWPLDDYFSLQADAEL
                     ARECGFDLSWRAAYTEGIDHMPEDDDTNYTVTGLAIYKQSGPGFTPVDVARFWLSNIP
                     IFHVCTAERVAYRNLVDGIGPPASAACRNVYREWIGAQIRADFFGYVCPGDPERAAGL
                     AWRDACISHVKNGIYGAMWVAAMLAAAYVTDDPEAVIRAGMAQIPARCRLTAGLEHVL
                     GLWQRRVDYWEAVEDLHARWKEDRSHDWCHTISNAEVVAMALLWGERDFGRTICYSVM
                     PGFDTDCNGATSGSVLGLMLGRKALPERWIAPMNDTLMTGVAGYHKVSLTAVAQETVE
                     LMG"
     gene            complement(4761..>5506)
                     /locus_tag="PLQ54_02415"
     CDS             complement(4761..>5506)
                     /locus_tag="PLQ54_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1793 domain-containing protein"
                     /protein_id="HOS92140.1"
                     /translation="TYTKTDWHIWTACLADTPEDFEAFVAPVARFLNATPERVPMTDW
                     YQTTDATMVGFRARPVVGGVFIKMLTDGGRWSKWRGRGHTDDSAWAPFPRTPRFEPLV
                     PCAQQGGVVWRYTTDRPGGDWFAPGYDDSEWLRGESGFGQEGTPGSVVRTAWRTPDIW
                     MRRTFELEAGEGEPVALWIHHDEAAEVYLNGVLAAKTSGYLTEYDVVDIAGAAQQRLC
                     PGVNTLAVHCHQTVGGQYIDVGLVRVVEP"
BASE COUNT          926 a         1925 c         1745 g          910 t
ORIGIN      
        1 cttcggggtt ccgggcgaac ccgccgggcc agtcttcctc gagccgtgtc aggtgtggag
       61 ccacggccac agtgccgttg gggaagtggc agaacaggta cggcgtcgtc cgggaaagta
      121 cttccgtgtt gtcgttggcc ggcaggtcgg cggccgaggg cggataggcc cccagggcat
      181 tgagcgtctc gaaccagttc cgcgtctcgt agcccaggct ggccgactgg tcatcgcgcg
      241 gccggtagcc cacgaaggcc agcgcccctc ccgcgatggt cccgcggacc gttcccacga
      301 ctcgccccgc gcagcgagcc gtcacttggg tcccctcgcg tggccccacg gggtgaatgt
      361 ggtccaccac ggcatccgtg aggatgatct gcgcctcgat gccctccagt gccccttcga
      421 aggtcactcg cccgcccgga gccatctccc ccagtccgtc gggcgacgcg accgggtcca
      481 cgccgaaaag gtccgcccac cgcgcccgaa ccggctcgcc atcacgccca agcagcggcg
      541 gcggcccgct ccagatcgcg cgcccgccgg cctcggccaa gcgcgacatc agatcaagca
      601 gctcgggccg cgggaagggc tcgaacagcg ccgccagcgt cgtgaaccgc cgcccgccca
      661 gctcgacggc accatcgaca agccggccca tctccagtag cttcgccggg gtcacgtagt
      721 tggcataggc gtactgcgtc atccacgaac cgaaccgttc ctccgtgctc accaggtcca
      781 gcgggtacaa catcagcacg ctcacgtcac gatgctgcat ctcctgtacc agggtgtgca
      841 tggtcatgtg accggtggtc ccgaacgctc tccccacgtg ttctctccgc caggccacct
      901 cgttcgggct gccccagtgg gcacagtacg aatagggcag ctcgttcacg atgcccgttg
      961 agcaggccaa cgccatgcca tagtagttcc gatccagcca cccacattcg gggtggtcat
     1021 tccccgttcc agtgaggaac tcgccccact tgaagtaatc gtggcacgcc gccgccgcct
     1081 ggtggaccgt gttcgaccag acgaatcgcg agtcgtactc atagcgaatg ctgttcgcgt
     1141 tccgcgcgcc actccggtag tagtcgatgg tcgggctctc ggcccacgtc gcgtgcgcgc
     1201 gggtgtacat ccgccggccc gtccgctcct cggcgtcccg ccgcgcggcc aggaacaggt
     1261 cgaccacccc gttttgcagc agctcgtagt agcgcgagcg gaacagtgcc gtgcggtgga
     1321 tgtcctccgc actggggcca aacacgtgcg gaatgggctc cgtggcggtt aggcccgcca
     1381 ccgcatcttc ctgcccgtac gtgaagtaga ccatgtactt ggccaggtct gcatactgcg
     1441 gcccgtatcg ctcggcgaat cgcctttcga gccccgggct cacgtaccgc agcgcgaact
     1501 gcccgtgttc gtggtggccg aagtagtccc agtcctgctg aatgtgcatc tcgtccgagt
     1561 agaacccact gagctcgaca cccgcatcca ggtaccggcc gatgagagcc ttgagatatg
     1621 gcagagcgga gtcgctgaaa tagtccatct ccggtgagcg atactgctgt accaccagca
     1681 cgtggtcgag ctcccccacg tcggtgcgcc ccgtgccgtg aacgcgaatc cgtcggccca
     1741 ccgatcccga ccggatcccc tcaaacgcct ccacccgggc cacgtcgctg atctcgacga
     1801 tggagtcctc ggcgacggcc aagcacgatg tcccgccgac ggggcgctgg ccgaaggcga
     1861 atacccgcac acccaggtgt tcgaggtcga tgggcccctt gttgttcacc cagcgcaggt
     1921 gttgccacat ctggacgctg aaggcgcccg tccgcgggtc ccgcaaaccc ttgcgatagt
     1981 ggtaccaccg gccccactcg ccagtctgtt tcccatacgc tgggccgatc tcgagcgggc
     2041 tgagtaggct gagctccagc ttgagccctc gctccttcgc gaagcgcccc acccgggcga
     2101 tgagctgcac gtactcgtcc atatcgggtg tcagcgtccg cgcgtggtcg atcccggggc
     2161 ggtactgggc gaagaagtgg tcgaaggcca cgaccagcgt gtgtgcgcca cgcgcctggg
     2221 cctcatcgca gatctggcga aggctgcggt tcatcgggtc tcggcccgtg gtgaggttga
     2281 ggaccttgtc gggatcgcca gccaggtcca cgagctcctg gtcgctcacc aggatgatcc
     2341 cggcgctggg caggtggaat gcccacgggc ccgggtagct taggcactcg ccggagaaga
     2401 cttcctgtgg ggctagttcg gccatgcgag tctccctctc cactcggcgg cctcggggcg
     2461 gccgacaccc ggcgcgattc gccccgcggc acgtgtcccc tgcgtgaccc ggcaagttgg
     2521 gccacggcga ggcgagtcaa tgctgggagg gtcgatgcgg tgtctggccg aagggtcatg
     2581 aaccacgggg ccacaggcca caactgagga ggcgtgtcat gcgtagagcg ggtttcacgc
     2641 tgattgagct gctcgtcgtc atcgccatca tcgctatcct ggctgccatc ctgttccctg
     2701 tgttctcgaa ggcccgggag aaggcgcgcc aggcctcctg cctctccaac ctcaagcagt
     2761 atggactggc tgccatgatg tacgctcagg actatgacga caccatggtg ccgacctaca
     2821 tctacgaacc gacggggccc gccacgggct gtgggaacct gacgggctgg ttccatctgc
     2881 ttgagcccta tgtcaagaac aaccagatcc gtgtgtgccc atccggccat cacacctgtg
     2941 tctggaacac cccgctcagc tatggcgtga actcgctcgt catgtaccgg ccgttggccg
     3001 tgatcacaaa ccccgctgac ctgatccata tcttcgagat gccttattat ctggagatct
     3061 gggaccccga tacctatgcg gactacggca cggccggggg ctatgtcgcc aagcggcaca
     3121 acgacggttt caacgcctcg ttctgtgacg gccacgccaa atggatcaag aacagcaagc
     3181 acagcatgtg gtacccgggt tagccacctg gctccacgac acccagggag tacgccgatg
     3241 ccgttaggag cacgcaccaa ctgggccagc caaccgtgga tgctcgtcga acacaacgac
     3301 ctcgaggtcg agtggcagca ggccacggaa gaagggcgcg acctggcctc ggtggaggcc
     3361 gagttcgcgg acctgagggc ttcgcagctt gacacggaag agctccgcgt ccgcgcggag
     3421 gcactgctcg accgcgtcca ggagctgccg cttgtggccg gctacgccta cgaggagccg
     3481 tccgacctcg acggcattcg cgccgtgcgg cccgaggctg ctctcccgcc caagggccga
     3541 cccgatcgcg ccaccctcga ggacaaggcc ctcggagcct ggtgtggccg cgccagcggc
     3601 tgcctgctgg ggaagcccgc tgagggccgg cgccgctggc agatcgagaa gtacctcaaa
     3661 gcacaatcgc gctggccgct ggatgactac ttctcgctgc aggccgatgc ggaactggcg
     3721 agggagtgcg gattcgacct ctcgtggcgg gccgcgtaca ccgagggcat cgaccacatg
     3781 cccgaggacg acgacaccaa ctacaccgtc actgggctgg ccatctacaa gcagtcaggt
     3841 cccggcttca caccagtcga cgtggcccga ttctggctgt cgaacatccc catcttccac
     3901 gtatgtactg ccgagcgcgt agcctaccgg aacctcgtcg acggtatcgg gcccccggcg
     3961 tcggccgcgt gccgcaacgt ctaccgcgag tggatcggcg cccagatccg cgctgacttc
     4021 tttggctacg tctgcccggg cgatccggag cgcgccgccg ggcttgcctg gcgcgatgcg
     4081 tgcatcagcc atgtcaagaa cggcatctac ggcgcgatgt gggtcgccgc catgctggcg
     4141 gcggcctatg tgaccgacga tcccgaggcg gtcatccgcg ccgggatggc ccagatcccc
     4201 gcccgatgcc gcctgacggc cgggctcgag cacgtgctcg gcctgtggca gcggcgcgtg
     4261 gactactggg aggccgtcga ggacctccac gctcgttgga aggaagaccg ctcccacgac
     4321 tggtgccaca ccatcagcaa cgccgaggtc gtcgccatgg cgctgctgtg gggcgagcgg
     4381 gacttcggac gcaccatctg ctactcggta atgccgggct tcgacaccga ctgcaacggg
     4441 gccacctccg gcagtgtcct gggcctcatg ctcggccgca aggcccttcc cgagcgttgg
     4501 atcgcaccca tgaacgatac gctgatgacc ggggtggccg gctaccacaa ggtctccctg
     4561 accgccgtgg cacaggagac ggtggagctg atgggctgag gatcggcgga gaccggcagc
     4621 caggccgtcc ggaggggccg gacgggaggg cccatccggc ccggcttgag ctcgtgcggc
     4681 cggcgggcgc tccgcgctga gcccccgaag gagcgcctcg ctgtataaga gacgtaagtg
     4741 cgggcctcgc gacccgaacc ctaaggctct accacccgca ctagccccac atcgatgtac
     4801 tgccccccga ccgtctggtg gcagtgcacg gccagagtgt tgacacccgg acacagcctc
     4861 tgctgagccg cgccagcgat gtccaccaca tcgtactcgg tcaggtaacc cgaagtcttc
     4921 gcggcgagaa cgccgttgag gtacacttcg gccgcctcgt catgatggat ccacagcgcc
     4981 accggctcgc cttcgccggc ttctagctcg aacgtgcggc gcatccagat gtccggcgtc
     5041 cgccacgcgg ttcgtaccac cgagccgggc gtgccctcct ggccgaaccc cgactcgcct
     5101 cgcagccatt ccgagtcgtc atagccgggc gcgaaccagt ctccgccggg ccgatcggtc
     5161 gtgtagcgcc agaccacgcc gccctgctgc gcgcacggca cgaggggctc gaaccggggt
     5221 gtccgaggaa acggcgccca cgccgagtca tctgtatggc cccgaccgcg ccacttcgac
     5281 caccgcccgc cgtcggtcag catcttgatg aacacaccgc cgaccacggg ccgcgcccgg
     5341 aaaccgacca tggtggcatc tgtcgtctga taccagtcgg tcatgggcac acgttcgggc
     5401 gtcgcattca ggaaacgcgc gacgggcgcc acgaaggcct cgaagtcctc cggagtgtca
     5461 gccagacagg ctgtccagat gtgccaatcc gtcttcgtat aggtct
//
LOCUS       DAOSPL010000080         5503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_14927_length_5503_cov_3.109031, whole genome shotgun sequence.
ACCESSION   DAOSPL010000080 DAOSPL010000000
VERSION     DAOSPL010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14927_length_5503_cov_3.109031"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLQ54_02420"
     CDS             <1..309
                     /locus_tag="PLQ54_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOS92141.1"
                     /translation="GIPWLRVPAERDGCVHVFHQYVVQTDHRPALQEHLANCGVSTAI
                     HYPKVIPDQPFYAADGFATVDLPVARQLTQTVLALPVHPMVSTEGIATVIDAVRSFVP
                     "
     gene            588..1487
                     /locus_tag="PLQ54_02425"
     CDS             588..1487
                     /locus_tag="PLQ54_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOS92142.1"
                     /translation="MAEVNQFLARNLPADADYFVHISDMHIGGGSESRYPPFGDSGLE
                     FLVAEVSPTPRAERAIREIRQLSPAPAFVVFTGDLSDHGQPDEIEQALSILASLDVPV
                     HITRGNHEWHREAFLHAVSGLACYEGARMGTMGFCYAFDGLGARHIVLDSEEIHTEGP
                     QFEWFRAQLAALGDTAGLVYIHRNLKRVGYPPSDYRAGFIQHNGEDIIALLQQHPTAQ
                     WVIGGHVHLNAWRTAGALNLVSLTALFYSNDDAYTTAQGYSSLYAHLVCMRHGAVEWL
                     ARKRLGGSTAIQRPTERDLLSLV"
     gene            complement(1493..4987)
                     /locus_tag="PLQ54_02430"
     CDS             complement(1493..4987)
                     /locus_tag="PLQ54_02430"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS92143.1"
                     /translation="MRIRRLTIGRFGHFADAVLPLETGPERGLRVIYGPNEAGKTTVL
                     HAVRHLLFGFPVRSPYNFLYDYGSFELTAELEFSSGERAEVRRVKGQGQTLFARVTGT
                     GEEGDERWLRRRLGEPTEELFANVFGFSLAELADEERSLGTDVRDALYGGSLGAVNLE
                     GIRKRLASEMEALMKPSPTATRPPVNAALSQYRKLTGSLSAATVQAPAFEHLQEQLRT
                     AESAASACRERVSALRRRQALLDRAARALPRQALRRARAAELESLVVPRGFDTSCSAR
                     FEEAVARAAALEREREQLEQQRQRVAQELSGVEVDTRLMGLQDRIVALFGGMDRARDA
                     ERDLPLRIDERAALERQIQDDLTSLRPGTAASQLSRFAPKTPLRLRFDELVREAEQLE
                     KQRRDRAGRAAELRERIRLADQRLAELGPAEDLTAIEALFEEQAEDEGHRQALEQGRT
                     LLKRLDTRLAAARRRLDPPCSQLETAHNLLVPRVEQVEAMEREWAGIDRELSQAQDRR
                     AEALARLREAEADLREVDQKHLPTPEGLTEARAHRERGWQLIRRAWLAGEDVTEDAAA
                     YADDSDLATAYEAAVAAADGCADELRKHANEVAERARIALELERRKEAARAADQAILE
                     AQERREKWTARWRAMWERCPFEPDSPTVMRAWLKDHAALVELMNDREGQALEVDRHAR
                     AVAAYEAQVRHALDDAEAPLADLLQRARRRLAEHRERESRRAAESERRESDAVALQAV
                     ERDLALLDEESRKWNTRWSAVTDELSLPVGIAPADAASTIRGLDGTRQHADRVRELTQ
                     RIDQMESALREFADEAADLARAAMPEAEGARPQEIAEQLHAALLRTLQAAEKRTQLQA
                     RQDDQEAALAAKSLEIQQWQRAASELLALAEAQDEAAFREVAARAARAEELRHEIDRL
                     DRELREIRAEGSEDDLDALLAQCDLAAIVDERQRVENELEAAEAAGEKANQEVGQARS
                     ALERIDGGTGAAAIREDIQATLAELRSHAERYAVLAVARKLLDDEVTRFAVQHQPELL
                     TRAGAVFAHMTGGRYSGLSRLLDERGTIMAIRPGGDQLAPWQLSTGAAQQLYLALRLA
                     YVQHYCARSEPLPLIMDDVLVNFDDARARATLHTLAELSPEIQVLFFTCHPHLVELAR
                     SVVPGLTPIEIPAPVGGQ"
     gene            complement(4984..>5503)
                     /locus_tag="PLQ54_02435"
     CDS             complement(4984..>5503)
                     /locus_tag="PLQ54_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92144.1"
                     /translation="RHGECGGGQPLDQQGRGGRVETRDRLDQEARAAEGRLLAARVTI
                     EGPFEGTAELYDDDRRQQLLAEIRAIPDHLGIDAWIEKVNLRTSPLVPLDELRRGEGV
                     VAELLRRTAALMRDDDALPGIQEALRPLVERAGPQLREAGLDLTDPRVLRDLVAQAEV
                     ALTLHLQGHLSS"
BASE COUNT          803 a         1945 c         1822 g          933 t
ORIGIN      
        1 ggcatcccgt ggctgcgcgt gcccgccgag cgtgatggct gcgtccacgt gttccaccag
       61 tatgtggtgc agaccgatca ccggcccgcg ctccaggagc acctggcgaa ttgcggcgta
      121 tcgacggcca tccactatcc gaaggtgatc ccggatcagc ccttctacgc ggcggatggc
      181 ttcgccacgg tggaccttcc ggtcgctcgc cagctcaccc agactgtact ggcgcttccg
      241 gtacacccca tggtctcgac cgagggcatc gcgacggtga tcgacgcggt ccggtccttc
      301 gttccgtagg ccgggacttc acacacatcg gtgggcacgg cattgggcag agggggtggc
      361 ggacagtggg cctcagccga cggaggttcc tgaggagtgc cgcgctggcc cccgccgcgc
      421 tgtgcgtgcc gccctgggtg gctggctgcg ctccggccac acacgcctcc ccgagcgacg
      481 ccgcggcata cgttgccctc gaggccccgg ctccgggcgc ttacgagacg cgcaacgacc
      541 ccaaggaatc acgccggtcg gcgcacgtct cttccgaagc ggagagcgtg gctgaggtca
      601 accagttcct cgcgaggaac ctgcctgccg acgccgacta cttcgtgcac atctctgaca
      661 tgcacattgg gggaggcagc gaatcgcggt atcccccctt cggcgactcg ggcctcgagt
      721 tccttgtggc tgaggtgtcg cctacgccgc gagccgagcg ggcaattcgc gagatccggc
      781 agctctcacc cgccccggcc ttcgtggtct tcaccggtga cctcagtgac cacggccagc
      841 ccgacgagat cgagcaggct ctgtccatcc tcgcgtcact tgacgtgccg gtccatatca
      901 cgagggggaa tcacgagtgg caccgcgagg ccttcctcca cgcggtgtcc ggattggcct
      961 gctacgaagg agcccggatg ggcacgatgg gcttctgcta cgccttcgat ggcctgggag
     1021 ctcgccacat cgtgctcgac tcggaggaga tccataccga agggcctcag ttcgagtggt
     1081 ttcgggcgca acttgcggca ctgggggaca cggctggact tgtgtacatc caccgcaacc
     1141 tcaagcgcgt ggggtacccg cccagcgact atcgcgccgg gttcatccag cacaatggcg
     1201 aggacatcat tgcgcttcta cagcagcacc ctaccgcgca gtgggtgatc gggggccacg
     1261 tgcatctcaa cgcctggcgg acggctgggg cgctgaatct cgtatcgctg acggcgttgt
     1321 tctacagcaa tgacgacgcc tacacaacgg cacagggcta cagcagcctc tatgctcacc
     1381 tcgtgtgtat gaggcacgga gcggtggaat ggctggcccg gaagcgtttg ggcggcagca
     1441 cggccatcca gaggcccacg gagcgagacc ttctgagcct cgtgtgagcg cgtcactgcc
     1501 caccaaccgg cgccgggatc tcgatcggcg tgaggccggg caccacactg cgagccaact
     1561 ctaccaggtg gggatggcat gtgaagaaca agacctggat ctcggggctc agctcggcca
     1621 gcgtgtgaag agttgcccga gcccgagcat cgtcgaagtt gacgagcaca tcatccatga
     1681 tgagtggcag gggttccgag cgggcgcagt agtgctggac gtatgcgaga cgcagcgcga
     1741 ggtacagctg ctgggcggca ccggtgctga gctgccacgg cgcgagctga tcgccaccgg
     1801 gccggatggc catgatcgta cctcgctcgt ccaggaggcg cgagaggccc gagtaccgcc
     1861 ctccggtcat gtgggcaaag actgccccgg cccgggtcag cagttcgggt tggtgctgga
     1921 ccgcgaagcg ggtgacctca tcatccagca gtttccgggc cacggcgagc acggcgtagc
     1981 gctccgcatg gctccgcagc tcggccaggg ttgcctggat gtcctctcgg atggcggccg
     2041 ctcccgttcc gccatcgatt cgttcgaggg cggaccgggc ctgacccacc tcctggttgg
     2101 ccttctcccc ggccgcctcg gctgcctcaa gctcgttttc gacgcgctgc cgctcatcga
     2161 cgatggcggc aagatcacac tgggcgagaa gtgcgtccag gtcatcctcg gaaccctcgg
     2221 cgcgaatctc tcgtagctcg cgatcgagcc tgtcgatctc gtgccggagc tcctcggccc
     2281 gggccgcgcg agccgcgacc tctcggaacg ccgcctcatc ttgcgcctcg gcgagggcca
     2341 ggagctctga agcggcgcgc tgccactgct ggatctcgag ggacttggcg gccagggccg
     2401 cctcctgatc atcctgcctc gcctgaagct gtgtgcgctt ctccgccgcc tggagggtgc
     2461 gcagcagggc cgcatgcaac tgctccgcga tctcctgagg ccgagcgccc tctgcttccg
     2521 gcatcgccgc gcgcgccagg tcggccgcct cgtctgcgaa ctcccgtagc gccgattcca
     2581 tttggtcgat gcgctgggtg agctcacgaa ctctgtcggc atgttgccgg gtgccgtcca
     2641 ggccgcggat ggtactcgcg gcatcggcag gcgcgatgcc gaccggaagg ctgagctcgt
     2701 ccgtgaccgc cgaccaacgg gtgttccact tcctggactc ctcgtccagc agggccaggt
     2761 cgcgctcgac ggcctggaga gccactgcat cgctctcgcg cctctcagac tcggctgccc
     2821 ggcggctttc gcgttcgcga tgctcggcaa gacggcggcg cgcgcgctgc agcagatcgg
     2881 ccagaggagc ctcggcgtcg tccagcgcat ggcggacctg ggcctcgtac gcggccacgg
     2941 cccgggcgtg gcggtccact tcgagggctt gcccctcgcg atcattcatc aactcgacca
     3001 gggcggcgtg gtccttgagc caggctctca tcaccgtggg gctgtccggc tcgaaggggc
     3061 aacgctccca catggcgcgc caccgggccg tccacttctc gcggcgctct tgtgcctcga
     3121 ggatggcctg gtcggcggcg cgggcggcct ctttccgtcg ctcgagctcc agggcgatgc
     3181 gtgcgcgctc agcgacctcg ttggcatgct tgcgcagctc gtcggcgcaa ccatctgccg
     3241 ccgcgacggc cgcttcgtag gccgtggcca ggtcgctgtc gtcggcgtac gccgccgcat
     3301 cctcggtcac atcctccccc gccagccacg cgcgccggat gagttgccat ccccgttccc
     3361 tgtgtgcccg agcctccgtc aggccctcgg gcgtgggtag gtgcttctgg tcgacttcgc
     3421 gcagatcggc ctcggcctct cgcaggcgcg ccagggcttc ggcccggcgg tcctgagcct
     3481 ggctgagctc ccggtcgatg cccgcccact cacgctccat tgcctcgacc tgctcaacgc
     3541 gtggcacaag taggttgtgg gccgtctcaa gctgcgagca cggcggatcg agtctgcgcc
     3601 gcgccgccgc cagccgggtg tcgagccgct tgagcaacgt gcgtccctgc tcgagtgcct
     3661 ggcggtggcc ttcatcctct gcctgttcct cgaatagggc ctcgatggct gtcagatcct
     3721 ctgccgggcc cagttcggcc aggcgctgat cggccagccg gatccgctcg cgcaactcgg
     3781 ccgcccggcc ggctcggtcg cggcgctgtt tctcgagctg ctcggcttcc ctgaccagtt
     3841 catcaaaacg caggcgcagc ggggttttcg gcgcgaagcg gctcagttgg ctcgcagccg
     3901 ttccgggccg aagggaagtc agatcatcct ggatctgacg ctccagcgcg gcgcgctcgt
     3961 cgatgcggag cggcagatcg cgctcggcgt cccgggcccg atccatgcct ccaaagagcg
     4021 ccacgatgcg gtcctggagc cccatgagcc tggtatccac ctcgaccccg gagagttcct
     4081 gggccactcg ctgacgttgt tgctcaagct gctcgcgctc ccgctccagt gctgcggccc
     4141 tggcaacggc ttcctcgaat cgcgccgaac acgacgtgtc gaatccgcgg ggaacgacca
     4201 ggctctcaag ttccgccgcc cgggcccggc ggagcgcctg cctggggagc gcccgcgcgg
     4261 cgcggtccag gagcgcctgg cgccgccgta aggccgatac ccgctcgcgg caggcactgg
     4321 cggcgctctc ggcggtgcgc aactgctcct ggaggtgctc aaaggccggc gcctgaacag
     4381 tggccgcgct caacgagccc gtgagcttgc ggtactgact gagcgcggcg ttcaccggtg
     4441 gcctcgtcgc cgtcgggctg ggcttcatca gtgcttccat ctcgctagcg agccgcttgc
     4501 ggatgccctc caggttcacc gccccgagac tgccgccgta gagggcgtcg cgaacgtcag
     4561 tcccgaggga ccgctcctcg tccgccagtt cggccagcga gaacccgaag acattcgcga
     4621 acagctcctc cgtgggttca ccgagccggc gccttagcca ccgctcgtcg ccctcttcgc
     4681 ccgtcccggt cacgcgggcg aacagagtct ggccttgccc cttgacccgc cgcacctcgg
     4741 cgcgctcgcc gctcgagaac tcgagctcgg ccgtgagctc aaaggagcca tagtcgtaga
     4801 ggaagttgta cggcgaacgc acggggaacc cgaacaggag gtgacgcaca gcatgaagca
     4861 ccgtcgtctt ccccgcctcg ttgggtccgt atatgacgcg cagcccccgc tccgggccgg
     4921 tttccagagg cagcacggca tcagcaaagt gcccgaagcg cccgatggtc agccgccgga
     4981 tcctcatgac gacaaatgcc cctgaaggtg cagggtcaac gcgacctcgg cttgggccac
     5041 gaggtcccgg agtacgcgag ggtcggtgag gtccagcccg gcttcgcgaa gctgcggccc
     5101 ggcgcgttcc acgagcggcc tcagggcctc ttggatgcct gggagggcgt cgtcgtcgcg
     5161 catcagggcc gcggttctcc ggagcagctc agcgaccaca ccctcgccgc gccggagctc
     5221 gtccagcggg acaagaggcg aggtccgcaa gttcaccttc tcgatccagg cgtcgatccc
     5281 gagatggtcg ggtatggcac ggatctcggc cagcaactgc tgacgccggt cgtcgtcgta
     5341 cagctccgcc gtaccctcga aggggccttc gatggtcacc cgcgccgcca gcaggcggcc
     5401 ctctgccgcc cgggcctcct gatcgagccg atcgcgggtc tcgacccgac caccacgacc
     5461 ctgctggtcg agcggctggc cgccgccgca ctcaccgtga cgc
//
LOCUS       DAOSPL010000081         5495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_14952_length_5495_cov_3.473897, whole genome shotgun sequence.
ACCESSION   DAOSPL010000081 DAOSPL010000000
VERSION     DAOSPL010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14952_length_5495_cov_3.473897"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PLQ54_02440"
     CDS             complement(<1..319)
                     /locus_tag="PLQ54_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92145.1"
                     /translation="MSADTMATDDRGTASEDGGLVTRSSPPTASLYVAVFLISAAVLI
                     FEIGLTRIFSVLLRFHFVFLAVSCAICGLGLGGMLSVSASRVVRQRLADGTVLAGLAL
                     ALGV"
     gene            complement(338..2617)
                     /gene="topA"
                     /locus_tag="PLQ54_02445"
     CDS             complement(338..2617)
                     /gene="topA"
                     /locus_tag="PLQ54_02445"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOS92146.1"
                     /translation="MGKPLIIVESPTKVRTLSRFLGGRYQVASSSGHIRDLPKSTLGV
                     DIAEGFRPRYRVLPDKQRVVRDLEKAAQKASRVFLASDPDREGEAIAWHIAQTLKLAD
                     ADRIEFNEITPEAVERALAHPRKLDMARVDAYHARRVLDRLVGYELSPLLWRKVHRRG
                     LSGGRVQSVALRLICEREREIQAFEAQEYWTIEAELTPKTARASFPAVMPFTKATRMD
                     IGSEGEARSIVDEVRGAEWRVQSVRKAERKRWPRPPLITSTLQRAASARYGFSSQRTM
                     TIAQSLYEGKDLGGGEASGLITYMRTDSVRVAQSAQEAAREYLAERFGPEYVPEKPPA
                     YRTRRGAQDAHEAIRPTLVTRTPESVAPFLTADELKLYRLIWEAFLASQMPPAVLDTV
                     TVDIAAGPRVFRASGYRVRFPGYMALQPERAPAPSSEKRAEAEPEDEAEENGAEHPLP
                     ELEEGQVLELVSVEPSQHFTQPPPRYTEAALISALEERGIGRPSTYAPTVEVIKARRY
                     ALIEDKRFRPTPLGFAVNDLLVQHFPDVVNAEFTAKMEGQLDAIEEEGAAWERIVAEF
                     WGPFETTLKRAQEEMQDVRLEPEKVGRDCPECGAPLVLRHGRFGEFVGCSAYPECSFI
                     EREAEAEQPTDEICEKCGRPMVRKRGRFGFFLGCSGYPECQNIRKPDRKPAQPTDIPC
                     PNEGCDGHLVLRQARRGNSFYGCSRYPKCRFTSSKKPVGRKCPKCGEQLVQAGTDEEP
                     TGVECSARGCDYQESAEAS"
     gene            complement(2686..3810)
                     /gene="dprA"
                     /locus_tag="PLQ54_02450"
     CDS             complement(2686..3810)
                     /gene="dprA"
                     /locus_tag="PLQ54_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOS92147.1"
                     /translation="MPGGDPEPISSRPPAELSYWLLLSRLSLPPPLVCDLLQCFGDAA
                     GVFAASREELRAVDRSAERLYRRVREAEQTTDLAEFTRQLQSMGARVVIRDDAEYPAL
                     LRRIHTPPPVLFVGGQLSALGGPCVAMVGSRAASAAGLRVATTLARGLAEAGLTVVSG
                     MAVGIDGAAHRGALDGGGPTVAVLGCSLEIPYPRANVDLRARIRECGALVSEFPPGTE
                     PRRWAFPQRNRLVSGMCMGTIVVEAPERSGALITADLALEQHREVMAVPGETESGRNR
                     GCHNLIRDGAVLVETAEDVVNALGLEPRRPSAPRPAPPPCELTPPERLALGALTREGA
                     LIEHVTAATGFPPAEVVSALMLLEMKGLAQRLPGGRYGLR"
     gene            complement(3825..4316)
                     /locus_tag="PLQ54_02455"
     CDS             complement(3825..4316)
                     /locus_tag="PLQ54_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92148.1"
                     /translation="MTAKLAKDTVSFAASPGWLEAALMSRFLLRLVLFGIIVGVPTYW
                     LFLFSQSQQNTALAWQRMDLPFFPYDLCASAPEVGLAALIGGFFLGVIGVFLLFGVLR
                     RPSRRVDTKQHIEAARRKIGELQAENEVLRRRLRQTAGDAPAATASRPSDDAGTSAPA
                     GSP"
     gene            complement(4370..>5495)
                     /locus_tag="PLQ54_02460"
     CDS             complement(4370..>5495)
                     /locus_tag="PLQ54_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92149.1"
                     /translation="WSTELLKPVDGLEGEEPQPVPIKSAAVIAGEYCLVRSENGVISA
                     LGLADGRIRWQYKVEPEPATPESGGALGTAPGAGGAAQPGGIGMAGGGMVGPVGGGLN
                     AGPSSVLRRSHSRRSVYGYGGMGWVGGNIGGPGNVPGGAGNTPGGRMGSGQAEEEIIR
                     DFLTETNSSLAVADGCVYVLSGESALYALRPSGVDLAPPKVEAAWVLVPTVEGGQFEE
                     HEFEAVNDLGGASTDQGYSEAIPAAAPVYVELTVEDEGSGIDPAGFAVSVPTVPQVSH
                     EYYPDEGLLVVTLGRTGGAAAIPEGTHVVDVQMRDFTGRSQHARVTIKVDPSKPQPTG
                     TARTGMGPGGGMMGPMGSGGMMGPMGGGGMMGPMGPMGGQ"
BASE COUNT          890 a         1968 c         1764 g          873 t
ORIGIN      
        1 agacgccgag agccagtgcg agacccgcca gcacggtacc gtccgccaac cgctgccgga
       61 caacccggct ggcggagacc gagagcatgc cgccgaggcc gagaccgcag atggcgcatg
      121 acacggccag gaacacaaag tggaagcgca gcagcacgct gaagatccgg gtgagcccga
      181 tctcgaagat cagcacggcg gccgagatga ggaataccgc cacgtatagt gatgctgtgg
      241 gcggggagga ccgagtgacc aacccgccgt cctcggatgc ggttccgcgg tcatcggtcg
      301 ccatcgtgtc ggcactcaca gtagctccgg ctccggccta gctcgcttcc gcagactcct
      361 ggtagtcgca gccgcgggcc gaacattcga ccccggtggg ctcctcatcg gtcccggcct
      421 gcaccagttg ctcgccgcac ttggggcact tccggcccac gggcttcttc gatgacgtga
      481 accggcactt ggggtagcgg ctacagccat agaagctgtt gccccggcgc gcctggcgga
      541 ggaccaggtg tccgtcgcag ccctcattcg ggcacgggat gtcggtgggc tgcgcgggtt
      601 tgcggtctgg tttgcggatg ttctggcact ccggatatcc cgagcaaccc aggaagaacc
      661 cgaatcgccc gcgcttcctg accatgggcc ggccgcactt ctcgcagatc tcgtcggtag
      721 gctgctcggc ctctgcctca cgctcgatga aggagcactc cgggtacgcg gagcagccca
      781 cgaactcgcc gaagcgcccg tgccgcaaca ccagcggggc gccgcactcg gggcagtctc
      841 ggccgacctt ctcgggttcg agccggacgt cctgcatctc ctcctgggcg cgcttgagcg
      901 tggtctcgaa cggcccccag aactcggcca caatccgctc ccaggccgcc ccctcttcct
      961 cgatggcgtc gagctggccc tccatcttgg ccgtgaactc ggcgttcacc acatcgggga
     1021 agtgctgcac cagcaggtca ttgacggcga acccgagcgg cgtgggccgg aagcgtttgt
     1081 cttcgatcag tgcgtagcgg cgggccttga tgacctcaac ggtcggcgca taggtgctgg
     1141 ggcgaccgat gccacgctcc tccaaagccg agatgagtgc cgcctccgta taccgcggcg
     1201 gcggctgggt gaagtgctgc gagggctcga ccgacaccag ctcgagcacc tgtccttctt
     1261 cgagctcggg gagtggatgc tcggctccgt tctcctcggc ctcatcctct ggttccgcct
     1321 cggccctctt ctccgaactc ggagcgggcg ctcgctcggg ctggagggcc atgtatccgg
     1381 ggaagcgcac gcggtacccc gacgcccgga acacgcgggg ccctgccgcg atatccacgg
     1441 tgaccgtgtc caacacggcc ggcggcatct ggctggccag gaacgcttcc cagatcaggc
     1501 ggtagagctt cagctcatcg gcggtcagga acggggccac cgactcaggc gtccgggtga
     1561 ccagtgtggg gcggatcgcc tcgtgcgcgt cctgtgcgcc acggcgtgtg cgataggcgg
     1621 ggggcttctc gggcacatat tcgggtccga accgctccgc cagatactcg cgggccgcct
     1681 cctgtgcgga ctgggcgacg cgcaccgagt cagtgcgcat gtaggtgatc agaccggacg
     1741 cctcgccccc acccaggtct ttgccctcat acaggctctg ggcgatggtc atggtccgct
     1801 gcgatgagaa gccatagcgc gccgacgctg ctcgctgcag cgtgctcgtg atgagcggag
     1861 ggcgcggcca gcgcttccgt tccgccttac gcaccgactg cacacgccac tcggctcccc
     1921 tgacctcgtc gacgatggac cgggcctcgc cctctgagcc gatgtccatc cgcgttgcct
     1981 tggtgaacgg catgacggca gggaagctgg cgcgagctgt cttcggagtc agctcagcct
     2041 cgatggtcca gtactcctgg gcctcgaagg cctggatctc gcgctcgcgc tcgcagatca
     2101 gcctcagcgc caccgactgc acacggccgc cggataggcc tcgccgatga actttgcgcc
     2161 acaacagggg tgagagctcg taccccacca gtcgatcgag cacccggcgc gcgtgatagg
     2221 catccacgcg agccatgtcg agcttccgcg gatgagcgag agcgcgctcg accgcctccg
     2281 gcgtgatctc gttgaactcg atgcggtcgg catcggccag cttcagcgtc tgggcgatgt
     2341 gccacgcgat ggcttcaccc tcgcgatcag ggtctgaggc gagaaacacg cggctggcct
     2401 tctgggccgc cttctcgagg tcgcgcacca ctcgctgctt gtccggcaga acccggtagc
     2461 gcggccggaa gccctcagcg atgtccacgc cgagcgtgga cttgggcagg tcgcgaatgt
     2521 gccctgacga cgaggccacc tggtacctgc cgcccagaaa ccggctcagt gtccggacct
     2581 tggtcggcga ctcgacgatg atcaacggct tacccactcg tgcaatcaac ccctggtgcg
     2641 tgtcaaccgc cgtccgcctc tcgaaggcgt cggctcactg cagatctagc gaagtccata
     2701 gcgcccgccg ggtaaccgct gggccagccc tttcatctcc aggagcatca gggccgagac
     2761 cacctcggcg ggagggaatc cggtggccgc ggtgacatgc tcgatcagcg cgccttcccg
     2821 cgtgagggca cccagcgcca ggcgttctgg cggggtcagc tcacacgggg gcggagcggg
     2881 ccggggggca gaaggcctcc ggggttcgag gcccagcgca ttcaccacgt cctcggcggt
     2941 ctccaccaac acggcaccgt cgcggatgag gttgtggcat ccacggttcc ggcccgactc
     3001 ggtctcgccg ggaacggcca tgacctcccg gtgctgctcc aaagccaggt ctgccgtgat
     3061 tagcgctcca cttctctccg gcgcttcgac cactattgta cccatgcaca tgccgctgac
     3121 aaggcggttg cgctggggga acgcccaccg ccgcggctcg gtgccggggg gaaactcgct
     3181 caccagggcc ccacactccc ggatcctcgc cctcagatcc acattcgccc ggggataggg
     3241 gatctcgagc gagcatccga gcaccgccac cgtcggcccc cccccgtcga gcgcgccccg
     3301 atgggcggcc ccgtcgatgc ccaccgccat cccgctcacc acggtcagcc cggcctccgc
     3361 caggccccgc gctagggttg tggccacgcg caggcccgca gcgctggccg cacgggagcc
     3421 caccatggcc acgcacggtc ccccgagggc actaagctgg cctccgacga acagcactgg
     3481 aggcggcgtg tgaattcggc gaagcagcgc cgggtactca gcgtcgtcgc ggatgaccac
     3541 tcgagcgccc atgctctgga gctggcgagt gaattccgcc aggtccgtcg tttgctcggc
     3601 ctcgcggacc cggcgataga ggcgttcggc cgatcggtcg accgcacgga gttcctcccg
     3661 gctcgccgcg aagacacccg ccgcgtcgcc gaaacactgg agcaggtcac acaccagggg
     3721 aggcggcaga ctgagccggg acagcagcaa ccagtaggac agttcagccg gtggtcgaga
     3781 cgagatcggc tccgggtcgc cacctggcac tgctctcggg gctcctaggg actgcctgcc
     3841 ggcgccgagg tgccggcgtc gtccgaaggt ctgctggccg tggccgccgg ggcatccccc
     3901 gcagtctgcc gaaggcgccg ccgaagcacc tcgttctcgg cctgaagttc gccaatcttg
     3961 cgccgcgcgg cctcgatgtg ttgcttcgtg tccacccgtc gtgacggacg ccgcaacacg
     4021 ccaaacagca agaacacgcc gatgacaccg aggaagaagc cgccgatgag cgcggccagg
     4081 cccacctctg gcgcactggc acacaggtcg taggggaaga acggtaggtc catgcgttgc
     4141 caggccaagg ccgtgttctg ctggctctgg gagaacaaga atagccagta ggtcggcacc
     4201 cccacaatga tgccgaacag cacgagccta aggaggaacc gcgacatcag ggcagcctcc
     4261 agccagccgg gagatgcggc gaagctcacg gtatccttcg cgagcttcgc cgtcaaccat
     4321 tcgtgctcgt gttctgtgac ccagtccccc ggcagagtac ggtgtgcggc tactggccgc
     4381 ccatgggacc catggggccc atcatcccgc cgccgcccat gggacccatc atcccaccac
     4441 tgcccatggg acccatcatt ccgccgcccg ggcccatgcc cgtgcgcgcc gtgcccgtgg
     4501 gttggggctt actgggatcg actttgatgg tgacccgggc atgctgagag cgcccggtga
     4561 agtctcgcat ctgtacatcg acgacatggg tgccctcggg aatggccgcg gcgccgcccg
     4621 tgcgtcctag tgtgaccacg agcaggccct cgtcagggta atactcatgg cttacttgcg
     4681 ggactgtggg cacgctcacg gcgaaaccgg cggggtcgat acccgatccc tcgtcttcga
     4741 ccgtcagctc gacatagacg ggcgccgccg ccggaatggc ctcgctgtag ccctggtctg
     4801 tgctggcccc tcccaggtcg ttgacggcct cgaactcgtg ctcttcaaac tgcccgccct
     4861 cgaccgtggg gacgaggacc catgcggcct caaccttggg cggagccaga tccacccccg
     4921 aaggcctgag cgcatagagt gcgctctcgc ccgacagcac atacacgcaa ccgtcggcca
     4981 cggccaacga gctgttggtt tcggtcagga agtcccggat gatctcctct tctgcttgcc
     5041 cggaacccat gcgcccgccg ggggtgttcc ccgcgccgcc ggggacgttg ccggggccac
     5101 cgatgttccc accgacccag cccatgcccc catagccata cacgctacga cggctgtgtg
     5161 agcggcggag aaccgacgag ggtcccgcat tcaggccacc cccaacgggg ccgaccatcc
     5221 cgccgccggc cataccgatc ccaccgggct gggccgcgcc ccccgcaccc ggagcggtcc
     5281 ccaatgctcc accgctctcc ggtgtcgccg gctccggctc caccttgtac tgccaccgta
     5341 tccggccgtc cgccaagccc agcgccgaga tgacgccgtt ctcactgcgc accaggcaat
     5401 actcgcccgc aatgaccgcc gccgacttga tgggcaccgg ctgaggctct tcgccctcaa
     5461 ggccgtcgac cggcttcaag agctccgtcg accac
//
LOCUS       DAOSPL010000082         5467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_15042_length_5467_cov_5.272912, whole genome shotgun sequence.
ACCESSION   DAOSPL010000082 DAOSPL010000000
VERSION     DAOSPL010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15042_length_5467_cov_5.272912"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PLQ54_02465"
     CDS             complement(<1..226)
                     /locus_tag="PLQ54_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92150.1"
                     /translation="MAEIPREVSGCPYCMQAIDDSDRPPPGAEPKPDERCTPFDEVIL
                     QYLARREIWLLLSVVLAPLALGLGIAGIWLA"
     gene            complement(263..3523)
                     /locus_tag="PLQ54_02470"
     CDS             complement(263..3523)
                     /locus_tag="PLQ54_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOS92151.1"
                     /translation="MPLEDLLHREDIPEDLRHQLRELLTERRLFHALLDHIPDTIYFK
                     DTQSRFIRTNRAHARVLGVQDPSEAEGKSDRDFFSPEDAAGFLEDEQRVMQTGEALLD
                     KTELVRYANGEARWCSTTKIPLYSDKGEAIGLVGITRDITDRVRSDEALRAAEQRQAR
                     LVQGLQTVMAVVQELMGLPDLDTVCRRGVELARERLGVERCSIFLREGDRFKGTYGTD
                     DHGKTTREYETSFVSADWSKHFREAVDGRRSWSVRQAEWLCTGEGETVEIGQGWVAST
                     PILGSDTLVGFMFNDAALTNAPVDEITQDLIAVYCSALGVVIERVRTEERLAAVSNAA
                     PVGIHAYDLGPDGVLVFRGANPAADVMLKMDHRQFIGRPIEDVFPALAGSEVLEHYRW
                     AAAYGEPWHVESLPYADGWIQGTFELHAFQTAPGQMAVMFSDVTERRQMEQDLRRTQF
                     AIDHVAEAAFWMDSLGRFVYVNEQACRSLGYSHAELLHMTIHDIDPNFPPEAWQAHWQ
                     DLKTHTTLVLQTSHRTKEGREFPAEVTANFVEFEGQEYNCAFARDITGRLRAQAALRE
                     SEQRYRLLAENATDVIASHDLDGTILYVSPSCRSAMGYEPEEMIGHKIYEFIVDDSRS
                     EVGARLSSIPSNHVVYTVQYQFMRKDGSLVWMETTARATLDPHSEEPMGLLSISRDIT
                     ERRRAEEERRQLEAELQHMQKLESLSVLAGGVAHDFNNILMGVLGNADLALMQLPPES
                     PARPLVQQVEAAAARAAELSRQMLAYSGRGTLMVEPIDLSRLVDEVFHLLQSSISKKA
                     LLQRNLSASPCLLRADATQLRQVVMNLVINASEALEDSPGVITIETGVAEIGAEELHR
                     AYRVDYELPPGRYVYLQVSDTGCGMAPETRERIFDPFFTTKFTGRGLGLAATLGIIRG
                     HHGAVVVDSELGKGSVFRVLFPWAEGLSMPEASAPVDSTGWKGWRGSGAVLVADDEAY
                     VRDIARHVLGMAGFEVVVAHDGQEAVERFAQRASEWVAVVLDLTMPRKTGEEVFDEIR
                     ALRADIPVVLCSGYGQQEAERRFAGKTLSAFLHKPYRPAELITKLREVLEAAERAV"
     gene            3750..4475
                     /locus_tag="PLQ54_02475"
     CDS             3750..4475
                     /locus_tag="PLQ54_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92152.1"
                     /translation="MVLGNVRTKLAVALGVSLAALAGLVWIAVAQEEVPAGEQAAATL
                     AEARQVMAAETTDPLADADDTERFVVNFRFIGGWWREDGPEPSFEDILGHPRGQIPPP
                     ISRQEFFDDQAMARIGNQSGKLDTRVSPVYSLVCSAQMPGRAELKAPDRYFYWEATAR
                     RELGDTVTLDLCWNPRKGPKATDEYFSQRYFHRTEDGQLKTGLRMKAECTIGRGHGCL
                     CLGPGPDETDLLLCYLLVEEAWP"
     gene            5110..>5467
                     /locus_tag="PLQ54_02480"
     CDS             5110..>5467
                     /locus_tag="PLQ54_02480"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOS92153.1"
                     /translation="MSLLKIGLLVTGLTALFVVVGHVIGGATGVVIALALALITNFAS
                     YWYSDRIILGMYGARPVPPKAAPELYGMLERLAQAAKIPVPRLYILPDPQPNAFATGR
                     SPAHAAVALNQGLLDRL"
BASE COUNT         1015 a         1747 c         1715 g          990 t
ORIGIN      
        1 tcgcgagcca gatgccggcg atacccagcc ccagagccag aggtgccaag accaccgaga
       61 gcagaagcca gatctctcgc cgggccaggt actggaggat gacttcatcg aagggggtac
      121 agcgctcgtc aggcttgggc tccgccccag gaggcggccg gtcggagtca tcaatggctt
      181 gcatgcagta ggggcagcca ctgacctcgc ggggaatctc tgccaagcaa cgcgggcact
      241 tcatcgccca ccggaggccg gactagacag cacgctccgc ggcctccaag acctcccgaa
      301 gtttggtgat gagctccgcc gggcgatagg gcttgtgcag gaatgcgctg agggtcttgc
      361 cggcgaaacg ccgctcggcc tcctgctgcc catacccgct gcagagcacg acgggtatat
      421 cggcgcgcag ggctcggatc tcgtcgaaga cctcttcgcc cgtcttccgt ggcatggtca
      481 gatccagcac caccgcgacc cattcggagg cgcgttgcgc gaaccgctcg acggcctctt
      541 gcccatcgtg ggcgaccacg acttcgaaac ctgccatccc gagcacatgc cgggcgatat
      601 cgcgcacata ggcttcatcg tcggccacca ggacagcgcc ggaacctcgc caacctttcc
      661 agcccgtgga atccaccgga gccgaggcct cgggcatcga caatccctcg gcccagggga
      721 acagcacccg gaaaaccgat cccttgccca actcgctgtc gaccaccacg gcgccgtggt
      781 gaccacggat gatccccagc gtcgcggcca acccgaggcc gcgtcccgtg aacttggtcg
      841 tgaagaaggg gtcgaagatt cgctctcgag tctcgggcgc catgccacag cccgtgtcgg
      901 agacttgcag atagacatag cgcccgggcg gaagttcata gtcgacgcgg taggcccggt
      961 gcagctcctc ggctccgatc tcggcaacgc ctgtttcgat ggtgatgacc ccgggggagt
     1021 cttcgagcgc ttctgaagca ttgataacca ggttcatcac tacctggcgg agttgggtcg
     1081 catcggcgcg caacaaacac ggactggccg acaggttgcg ctggagtagt gccttcttcg
     1141 agatggagga ctggagcagg tgaaagacct catcgaccaa ccgcgagagg tcgataggct
     1201 cgaccatgag cgtgccacgg ccggaatagg ccagcatctg gcgtgacaac tcggccgccc
     1261 gggcggccgc cgcttcgacc tgttgcacca aggggcgggc gggcgactcg ggcggcaact
     1321 gcatcagcgc caggtcggcg ttccccaaca cacccatcag gatgttgttg aagtcgtggg
     1381 ccacaccgcc ggccagcaca ctgaggctct cgagcttctg catgtgctgc agctcggctt
     1441 caagctgccg tcgctcttcc tcagcacgtc tgcgctcggt gatgtcccgg gagatggaga
     1501 gaaggcccat cggctcctct gagtgcggat cgagcgtggc ccgagcggtc gtctccatcc
     1561 acaccaacga gccgtccttt ctcataaact ggtactggac cgtgtagacc acatggttcg
     1621 aggggatcga gctcagtctc gcccccacct ccgaacggct atcgtccacg atgaactcat
     1681 agatcttgtg tccgatcatc tcctcgggct cataccccat ggcgctccgg catgatggag
     1741 agacgtacag gatggtgcca tcgagatcat gggaggcaat gacatccgta gcattctcgg
     1801 ccagcagacg atagcgctgt tcactctcgc gcagggccgc ctgggctcgc agtcgcccgg
     1861 tgatgtctcg ggcgaaggcg cagttgtact cctggccctc gaactcgacg aagttggcgg
     1921 tgacctcagc cgggaactcc cggccctcct tggtgcgatg ggaagtctgc agaaccagag
     1981 tggtgtgggt cttcaggtcc tgccaatggg cctgccaggc ctcgggagga aagttcgggt
     2041 caatatcgtg aatggtcatg tggagtagct cggcatggga gtaccccaac gagcggcatg
     2101 cctgctcgtt cacatagacg aaccgcccca gggaatccat ccagaaggcc gcttcggcta
     2161 cgtggtcgat ggcgaactgc gtgcgccgga gatcctgttc catctggcgg cgttcggtga
     2221 catcggagaa catgaccgcc atttggcccg gagcggtctg gaatgcgtgg agctcgaagg
     2281 tgccttggat ccatccatcg gcatagggta gcgactccac gtgccagggt tcgccatagg
     2341 ccgcggccca acgatagtgc tccaggacct cactgccggc cagggcgggg aagacatcct
     2401 cgatgggacg gccgatgaac tgccggtggt ccatcttgag catgacatcg gcagcgggat
     2461 tggccccccg aaagaccagg acaccatcgg gtccgaggtc ataggcatgg atgccgacgg
     2521 gggccgcgtt ggacacggcc gccagccgtt cctcggtccg cacgcgctcg atgaccacac
     2581 ccagagccga gcagtatacg gcgatgaggt cttgagtgat ctcatcgacg ggggcattcg
     2641 tgagagccgc atcgttgaac atgaagccga cgagcgtgtc cgaccccagg atcggtgtgg
     2701 aggccaccca cccttgaccg atctcgaccg tctcgccctc gccggtacag agccactcag
     2761 cctgccggac gctccaactc cttcggccat cgacggcttc ccggaaatgc ttggaccagt
     2821 ccgccgagac gaaggaagtc tcgtactcgc gagtggtctt gccgtggtca tcggtgccat
     2881 aggtgccctt gaagcgatcg ccttcacgga ggaagatgct gcagcgctcc accccgagcc
     2941 gttcccgcgc caactccacg cctcgacggc acaccgtgtc gagatcgggg aggcccatca
     3001 gctcctgaac cacggccatg accgtctgaa gcccctgcac cagccgggct tgacgctgtt
     3061 cggccgcccg gagggcttcg tccgaccgaa cgcgatcggt gatgtcccgg gtgataccca
     3121 ccaggccaat ggcctctccc ttgtcgctgt agagagggat cttggtggtg gagcaccagc
     3181 gggcttctcc attggcatag cggaccagct ccgtcttatc gagcagcgcc tcgccggtct
     3241 gcattacccg ctgttcgtcc tcgaggaacc cggcagcatc ctcggggctg aagaagtcac
     3301 ggtcgctctt gccctcggct tcggaggggt cttgaacccc caggacccta gcatgagccc
     3361 ggttggtccg gatgaagcgc gattgagtat ctttaaagta gatggtatcc gggatgtggt
     3421 cgaggagggc atggaacaag cgccgctccg tcagtagctc acggagctga tggcgcaggt
     3481 cctcgggaat atcttcccgg tggagtaggt cttccagggg catcaagagg cccccctccc
     3541 aggggtgaac acggcctccc ccttttcgtg cccttccgcc gaagacctat cgcgccgggg
     3601 aaccagcatt tggcctcgag ttgtacttgc ggtgcactcc tctgcatgga cttacgttgc
     3661 caattccggc ccgagaagga gttgcgcccc ccccctgaac taatcggtac gatatctact
     3721 gtgattcatg ggaggaggtg aatgcaagca tggttctcgg caatgtccgg acgaagctag
     3781 cggttgcgct gggagttagc ctagccgccc ttgccggtct cgtgtggata gctgtcgcgc
     3841 aagaggaagt gcccgcaggc gaacaagctg cggctacgct ggcagaagcg cgccaggtca
     3901 tggccgccga aaccacagat cccttagcgg acgctgacga tacggagcgg tttgtggtga
     3961 acttccggtt catcggcggc tggtggaggg aagacgggcc ggagccaagc ttcgaggata
     4021 tcctgggcca cccaagagga cagataccgc cccccatcag ccgccaggag ttcttcgacg
     4081 accaagcgat ggcccgcatt ggcaaccaaa gcggaaaact cgatacccgc gtgagcccgg
     4141 tctactccct ggtctgttct gcccagatgc ccggccgagc agagctcaaa gcacccgatc
     4201 gctacttcta ctgggaagcc accgctcgac gagagctggg ggacaccgtc acccttgacc
     4261 tgtgctggaa cccgaggaag ggcccaaagg ctactgatga gtacttcagc caacggtatt
     4321 tccatcgaac ggaggatggt cagctcaaga cgggcctccg gatgaaggca gagtgcacca
     4381 ttggccgcgg ccacggctgc ctgtgccttg gccctgggcc cgacgaaacg gatctgcttc
     4441 tctgctatct gctcgtggaa gaagcctggc cgtgatgcct aaggacgacc ctgtgcaacg
     4501 gtacaagctc gtgacagctg cgaccatcct caccatcggc gccgtgcctg ccgcgtctga
     4561 ctctcacacc tcctgtgcct ggcggaaatc acgaggcaag ggcgggaaca ggcgctctgt
     4621 tcaactggga cgatgtcact accaatgaca gccatcactt ccgcgtcgac ccatcgcctg
     4681 atggcggtcc cggcgggccc ccggggggcg acgctctggg ccaaggcaac gactcatggt
     4741 aagtgctgca tcggagcctg atgtctgata gtgggcggtg gcctcggcct ccgcccactt
     4801 tctgggcggg gcactgccta ttgcccgtct gtccaagcac aggcagccca gtggcccact
     4861 acgagcattc ctgggtggac aggaccaacg ggttcccctg aagacctatc gcggcgggga
     4921 accagcgcac gtctttcaag tgttctatat agacgaaggg gagtagcctc cactcggtcg
     4981 agtgggttgg gaacatcgtc aggacgctct cttgcgagcc ggtgttccga gaacgagcgc
     5041 gagaccttca tcgccgcccg ggggcgcggt ggaggtcttt tctgcgccgc gggagagggg
     5101 gactgtcgga tgtctctact caagattggc ctcttggtca cgggcttgac cgccctcttc
     5161 gtcgttgtcg gtcatgtcat tggcggcgcc accggcgtgg tcatcgccct ggccctggcg
     5221 ctgatcacta acttcgccag ttactggtat agcgaccgga tcattctggg catgtatggg
     5281 gcgcgtcccg taccgcccaa ggcggctccg gagctctatg gcatgttgga gcggctggca
     5341 caggcggcga agatccccgt gccgcggctc tacatccttc cggatcccca gcccaatgcc
     5401 ttcgccaccg ggcggagccc cgctcacgcc gccgtggcgc tcaaccaggg gctactcgat
     5461 cgcctcg
//
LOCUS       DAOSPL010000083         5416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_15215_length_5416_cov_3.247342, whole genome shotgun sequence.
ACCESSION   DAOSPL010000083 DAOSPL010000000
VERSION     DAOSPL010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5416
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15215_length_5416_cov_3.247342"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PLQ54_02485"
     CDS             complement(<1..367)
                     /locus_tag="PLQ54_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOS92154.1"
                     /translation="MEASIADLRQQVASELREVEDQLATVLQSRVETVPAIYHHARSG
                     GGKRLRPSLLILSAQSVAGDDYAARVSRSDLIHFAAAVEMVHVASLMHDDVVDGSDLR
                     RGRPTANALWRGKLCVFAAD"
     gene            complement(437..1099)
                     /locus_tag="PLQ54_02490"
     CDS             complement(437..1099)
                     /locus_tag="PLQ54_02490"
                     /inference="COORDINATES: protein motif:HMM:NF003993.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOS92155.1"
                     /translation="MRANPGPRLTSPIRRVPDPTVGRLSTYLHCLRALERDGITTVSS
                     AEMADHTGINAAQIRKDVSYIGELGRPGVGYDVGHLKRCLMERMGLGQPHGVLLVGAG
                     SLGSALCSYPGFAEQGFFIIAVFDSSPAKVGRRLGSLEVLSMEELARRNRDLRARIGV
                     IAVPRDAAQGVADCLVAAGIRGILNFAPVKIEVREGITVRNVDLAQEFEALSYYMASA
                     TD"
     gene            complement(1186..2154)
                     /locus_tag="PLQ54_02495"
     CDS             complement(1186..2154)
                     /locus_tag="PLQ54_02495"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOS92156.1"
                     /translation="MASRWLPFEWPLMEVEEELHALRTGGADASPDLAARVQALEARR
                     QELLQEIYSDLTAYQKVQLARHPRRPYALDYARLMFDDFVELHGDRRFGDDKAMIAGL
                     ATLAGRAVAVVGHQKGRDVSERKERNFAQGRPEGYRKAQRVMQMAERLRMPVISLVDT
                     PAADCLTEAESRGISEAIAEGQMVMSQLRTPILVTILGEGGSGGAIGIGVGDRILMLE
                     YAIYSVIPPEGYASIVWRDPSRVAEAAEAARITARDALALGVVDEVVPEPPGGAHADP
                     EGMAAALKARLCHHLAELDAVPPDELVDQRYAKFRAMGVYEELAIP"
     gene            complement(2179..3060)
                     /locus_tag="PLQ54_02500"
     CDS             complement(2179..3060)
                     /locus_tag="PLQ54_02500"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HOS92157.1"
                     /translation="MDGKRDESMAVEPGLQPRSEYREIADRIPDGLYTKCDSPDCGEL
                     LYRKDLEKSLWVCPKCGRHFRLGARDRLALLLDEGSFSEFGANLISCNPLGMPDYDSK
                     LARYRKATGENEAVITGLAAIGGHPVVVGITDPAFLMGSMGSVVGEKIARAMERAIEL
                     RHPCLMISGSGGGARMFEGILSLMQMAKTCAAVGRMQKAGIFYITVLTDPTMAGVYAS
                     WASLGDVVIAEPGAMIGFAGQRVYANTGSGMDPRIQTSEHQHACGLLDLIVPRAQLRS
                     TLERLRAIAYASHTHRK"
     gene            3507..4517
                     /locus_tag="PLQ54_02505"
     CDS             3507..4517
                     /locus_tag="PLQ54_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOS92158.1"
                     /translation="MAARARVSTATVSAALQPDAESSSVSPETRQRVLAAAEELGYDY
                     ARLRPRSTRPTTVAVFTAGNVHPNVSALSYAILLELGERFAHHGIRMVVESGHGPGSR
                     RPDAAADMLRRGDVDAGIVIALWDWARHMRSWGAPSVFLGAAPSEAGICQVCVDDELG
                     GRLVAEHLWSLGHRRVGVVHMSSDGWGADRERGFRSVWSQKGLSLADRCCVSLPSTTE
                     TSAVETVRRVLTASEDQDGPVTALFCDADWVAAHAIRTLQSDGYKVPDDISVVGFDDA
                     PFATMLSPQLTTVRQPIPELSALAAALVVEQMSLGAPMAKSYTLPCELVVRGTTAPPA
                     GV"
     gene            4750..4959
                     /locus_tag="PLQ54_02510"
     CDS             4750..4959
                     /locus_tag="PLQ54_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92159.1"
                     /translation="MLRRHVVLVIAFVLMICVAGLVGCKKSSPPPDAPKTGDEMMEKW
                     KTMKAQGMKMPADSMPKDVKGSSAE"
     gene            4997..>5416
                     /locus_tag="PLQ54_02515"
     CDS             4997..>5416
                     /locus_tag="PLQ54_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS92160.1"
                     /translation="MRFLEVFLVTKRGFTLIELLVVIAIIAILAAILFPVFAKAREKA
                     RQTSCLSNLKQIGNATMMYVQDYDERFGGAGCSNCGANVPPANMMPQARVMPYVKNVQ
                     IFDCPSGTGVGKLTARDGYAWQGGWAYTAEFAGQEVDY"
BASE COUNT          896 a         1858 c         1766 g          896 t
ORIGIN      
        1 agtccgccgc gaacacgcac agcttgccgc gccagagggc attcgccgtg gggcggccgc
       61 ggcgcagatc ggagccgtcc accacgtcat cgtgcatcag cgaggcgaca tggaccatct
      121 cgaccgcggc ggcgaagtgg atcaggtcgc tgcgggatac ccgggccgcg tagtcgtcgc
      181 ccgccaccga ctgggcgctg agaatcagca ggctggggcg aaggcgctta ccaccgccac
      241 tccgtgcgtg gtggtagatg gccggcaccg tttcgacccg gctctgcagc acggtggcaa
      301 gctgatcctc cacctcccgc agctccgaag ccacctgctg acgcaagtcg gcgatggatg
      361 cctccaagct cttacctctt tcgtatgggt cagggccagt gcggacccca gcgccagaca
      421 gccagtggcg ccggcttcag tctgtcgccg aggccatgta gtacgacaac gcctcaaact
      481 cctgcgccag atccacgttc cgcaccgtga tcccctcacg gacctcgatc ttgacgggag
      541 cgaagttgag gataccccgg atcccggccg ccaccaggca atcggcaacg ccctgagcag
      601 catcccgcgg gaccgcaatg actccgatcc gggcgcgcag atcccgattc cggcgagcca
      661 gttcctccat cgagagtacc tcgaggctac cgaggcgtcg acccaccttg gccgggctgc
      721 tgtcgaacac ggcgatgatg aagaacccct gctcggcgaa gcccgggtag gagcagagag
      781 cggatccaag gctaccggcg ccgaccagta acacgccatg gggctggccc aggcccatcc
      841 gctccatcag gcatcgcttc aggtgcccca catcgtagcc gacgcccggt cggccgagct
      901 cgccgatgta cgacacatcc ttgcggatct gtgccgcgtt gatccccgtg tggtccgcca
      961 tctccgccga cgacacggta gtgatgccgt cgcgctccag cgcgcgcagg cagtgcaggt
     1021 acgtcgagag acggcccacc gtcggatcgg gaacacgacg gatggggctc gtcagcctgg
     1081 gaccggggtt tgcgcgcacc gcccggactc ctcttcgttt cgtgcatgcc agggggcgtc
     1141 gggtgccagc cgccttcgtg ggtggggcgc ggggccgtgc ggcgcctagg gaatggccag
     1201 ctcctcgtag acacccatgg cgcggaattt ggcatagcgt tgatccacga gctcgtcggg
     1261 tggcacggcg tccagctccg ccaggtggtg gcacaaccgg gccttgagcg ccgcggccat
     1321 gccctccggg tctgcatggg cgccgcccgg gggctcggga accacctcgt caaccacgcc
     1381 gagcgccagg gcatcccgcg ccgtgatgcg tgccgcctcc gccgcctcag cgacccggct
     1441 cgggtcgcgc cacacgatgg aggcatagcc ctccggcggg atcaccgagt agatcgcata
     1501 ctcgagcatc agaatccggt cgcctacgcc gataccgatg gcaccgccgc taccaccctc
     1561 ccccagaatc gtgaccagga tcggcgtgcg cagttgcgac atcaccatct gcccctcggc
     1621 gatggcctcg ctgatcccgc gtgattccgc ctcggtcagg cagtcggccg cgggcgtgtc
     1681 caccagcgag attacgggca tccggagccg ctcggccatc tgcataaccc gctgagcctt
     1741 ccggtatcct tcgggccgcc cctgggcaaa gttgcgctcc ttgcgctcgc tcacgtctcg
     1801 gcccttctga tgaccgacca cggccacggc tcgccccgcc agcgtcgcca ggccggcgat
     1861 catcgccttg tcgtcgccga agcgccggtc gccatggagt tccacgaagt cgtcaaacat
     1921 cagacgggca tagtccagcg cgtacgggcg acgtggatgg cgagccaact gcaccttctg
     1981 gtacgccgtc aggtcgctgt agatctcctg cagcagctcc tgccgccggg cttccagggc
     2041 ctgaactctg gcggcgaggt cgggcgacgc atcggcgcct cccgttctca acgcatgaag
     2101 ctcttcttca acttccatga gtggccactc gaaggggagc caccggctgg ccacaaactt
     2161 gttggtcagg gtcggcacct acttccggtg ggtgtgcgag gcataggcga tggcccgcaa
     2221 acgctccagc gtcgaccgca actgcgcccg gggcacgatc aggtcgagca ggccacaggc
     2281 gtgctggtgc tccgatgtct ggatgcgcgg gtccatgccc gatcccgtgt tggcgtagac
     2341 ccgctggccc gcgaaaccga tcatcgcgcc gggctcggcg atgaccacgt cgcccaggct
     2401 ggcccaactg gcgtacacgc cagccatggt cgggtcggtg aggaccgtga tgtagaagat
     2461 ccccgccttc tgcatccggc ccacggccgc gcaggtcttg gccatctgca tgagcgacag
     2521 gatcccctcg aacatccggg ccccgccgcc cgaacccgag atcatcaggc acgggtggcg
     2581 aagctcgatg gcgcgctcca tggcccgggc aatcttctcc cccacgaccg accccatgct
     2641 gcccatcagg aacgctggat cggtgatgcc caccaccacc gggtgcccgc caatggccgc
     2701 cagccccgtg atcaccgcct cgttctcacc ggtggccttc cggtagcggg ccagcttgct
     2761 gtcgtagtcc ggcatcccca gagggttgca gctgatcagg ttcgcgccga actcgctgaa
     2821 gctaccctca tcgaggagca aggccagtcg atcccgggca cccagccgga agtgccggcc
     2881 gcacttggga cacacccaga ggctcttctc cagatccttc cggtagagca gctcgccgca
     2941 gtcggggcta tcgcacttgg tgtagaggcc atcagggatt cgatcggcga tctcgcggta
     3001 ctcgcttcgg ggctgcagcc cgggctccac ggccatagac tcatcccgtt tcccgtccac
     3061 ggtcggcctc gaagaacgcg acggcagagt cagtactctg gtcgggatta tacccgaagg
     3121 gtgaggcacg gcaaaggaag gcgatacgcc agtcaggatc cccctcgctc gcacccaagg
     3181 ccacaggctt ccccagaggc catcccggta ctcggcgggg gctcggagcg tggcgcggcg
     3241 actcagccat cggtggccgc ccaaagcgtg ctggaaaccc accgcccgga agcctccgcc
     3301 gtgcgtcctg tggcgcactc gccacaccgg cccaagccgc cccatcgcgc gtcggcaaac
     3361 tgaagtcgcg tactgagaaa agcattgaca cggcacggct aacatgatag gtatacgaac
     3421 aatggaatta tgctgaacgt acggctgatt gttcagcaaa cgaggtgggc cgtctgtccg
     3481 gaagaggcca acccacactg cgagacgtgg ccgcccgcgc ccgggtgtcg acggcgaccg
     3541 tctcggctgc tctgcagccc gacgcggaat cgtcatcggt gagccccgag actcgccagc
     3601 gcgtcctggc cgccgccgag gagctggggt acgactacgc ccgacttcgg ccgcgatcga
     3661 ctcgaccgac cacggtcgcg gtgttcaccg ccgggaacgt gcaccccaac gtctccgcgc
     3721 tctcctacgc cattcttctc gagctcggcg agcggtttgc ccaccacggg atccgcatgg
     3781 tggtcgagag cgggcacggg cctggcagcc gtcggcccga cgcggccgcc gacatgctcc
     3841 gccggggcga tgtcgatgcg ggtatcgtga tcgctctgtg ggactgggcg cggcacatgc
     3901 gctcttgggg cgcgccttcg gtcttcttgg gagccgcacc ctcggaggcg ggcatctgcc
     3961 aggtgtgcgt cgacgatgag ttgggcggcc ggcttgtggc cgagcacctc tggtcgctgg
     4021 ggcaccggcg cgtcggcgtg gtgcacatgt cctccgacgg ctggggcgcg gaccgagagc
     4081 ggggcttccg atccgtctgg tcacagaagg gcctctccct ggccgaccgc tgctgcgtat
     4141 ctctcccgtc caccaccgag accagcgccg tcgagaccgt tcgccgggtg ctgacggcat
     4201 ccgaggacca ggatggcccg gtcacggccc tcttctgcga cgccgactgg gtggccgctc
     4261 acgcgattcg gaccctgcag tccgatgggt acaaggtccc ggatgacatc tccgtggtgg
     4321 gcttcgacga tgctcccttc gcgaccatgc tgtccccgca gctcaccacc gtgcggcagc
     4381 cgattcccga gctctccgca ctggccgcgg cgctcgtggt cgagcagatg agtctgggtg
     4441 cgccgatggc caagtcttac acactgccgt gcgagctggt ggtgagaggg acaaccgctc
     4501 ccccagccgg ggtctgaggc tgccccggcg cgcgggggcg agtagacgac gaggatgacc
     4561 gccaaaggtc tgtgagctgc tcagctcacc tgcctttgtg tccatggcgc ctcgcgagaa
     4621 ctgggcataa gagaaggggg gagtgcagga gtcattgggc gttcttggcc cggccatcgc
     4681 cattgccggc ccgctcgggc cgatggagac gcgcccttcg tgggcgccca ggaaggaagt
     4741 gcagtcccgt tgctgcgacg tcatgtcgtg ttggtcatcg cctttgtgct catgatctgt
     4801 gttgctggcc tggtcgggtg caagaagagt tcgcccccgc ccgacgcgcc caagacgggc
     4861 gacgagatga tggagaagtg gaagacgatg aaggcccagg gcatgaagat gccggccgat
     4921 tccatgccca aggatgtcaa gggatcctca gcggagtgac gtggtggctg ggctcggcct
     4981 cgccgagaca gcatgagtgc gattcctgga ggtgttcctg gtgacgaagc gtgggttcac
     5041 gctgatcgaa ctcctggttg tcatcgcgat tatcgcgatt ctcgcggcca ttctcttccc
     5101 ggtcttcgcc aaggcccgag agaaggcccg gcagacctct tgcctgagca acctgaagca
     5161 gatcggcaac gccaccatga tgtatgttca ggactacgac gagcggttcg gcggcgcggg
     5221 ctgcagcaac tgcggcgcca atgtgccccc cgcgaacatg atgccccagg cccgggttat
     5281 gccttacgtc aagaacgtgc agatcttcga ctgcccctcg ggcaccggag taggcaagct
     5341 gactgctcgg gacggctacg cctggcaggg cggttgggcc tacacggctg agttcgctgg
     5401 gcaggaagtc gactac
//
LOCUS       DAOSPL010000084         5385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_15320_length_5385_cov_3.450844, whole genome shotgun sequence.
ACCESSION   DAOSPL010000084 DAOSPL010000000
VERSION     DAOSPL010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15320_length_5385_cov_3.450844"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="PLQ54_02520"
     CDS             complement(<1..1254)
                     /locus_tag="PLQ54_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOS92161.1"
                     /translation="MLLHGLFGALVSVALAAGAVAAAGEINAKDLGAAGDGATDDTAA
                     IQAALDQAGAAGGGVVSLPSGKYRLDGHLTIPTGVTLSGVWEAPHFSTPEVGTVLLAY
                     EGRGQEDGPPLLMLETNSTVRGLTVVYPEQRASQITAYPWTIQGRGTHLNVSDVTLLN
                     PYKGIDFGTYAHEMHYIRNVYGCPLKIGVHLDKCTDIGRVENVHFNPNSWTRSGMDTA
                     PQGDDGARLVEYMQANLVAFEIGRSDWEFMLNTFSWGCRVGYRFFESEAGPTNGNFLG
                     IAADWAVIPLLVESTQPPGLLITNGEFVGSPACEAVVRIAPSNSGVVQLANCSFWGPH
                     QKIVEAAGNGLVSLSQCNFCQWANRPPAEGADPVPAIDVISGSATISGCLFQQAKDHI
                     RLREGVVSAVVMGNHTRGPARFIIET"
     gene            1388..2557
                     /locus_tag="PLQ54_02525"
     CDS             1388..2557
                     /locus_tag="PLQ54_02525"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HOS92162.1"
                     /translation="MNEIALRYGCNPNQKPARAYVESGEIPFEVLNGAPGYINLLDAL
                     NAWQLVRELRDALDRPAAASFKHVSPAGAAVAVPLSAELRRAYMVDDLDLSPLATAYA
                     RARGADQMSSFGDFAALSDVVDVSTAKLLRREVSDGVIAPGYEPEALELLKQKKDGKY
                     LVMAMDPGYVPPAQEVRDVFGLRLEQGRNDVTIGPALLERIVTQRRELPPEAVRDLIV
                     ATVALKFTQSNSVGFAKDGQVIGMGAGQQSRIHCTRLAGEKADTWWLRQHPKVLDLPF
                     RPGVKRPDRINAIVQYLWADWAPAERRAWEAVFEPVPELLTEAERRDWLDQLTGVALS
                     SDAFFPFSDCIYRAARSGVSYVVETGGSVRDDAVIAAADECGMVMAFSGVRLFHH"
     gene            complement(2631..3242)
                     /locus_tag="PLQ54_02530"
     CDS             complement(2631..3242)
                     /locus_tag="PLQ54_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92163.1"
                     /translation="MAIDRKRIIDALSRVQVSADDYGLIPAFNVFVNQLPCSFWNSFA
                     ERLTRQVGPEMLEAAEWLLVDAARTCGYHTGYGIITSEEWKAVVGPMIEKVPEDILHG
                     AFAVFTAWGWAKSEIIELVPNERMVVRAYDYYESDVVTFGRSTKPSAYMITGVCAAFM
                     GLAYGGEYDPAGGAAMRAFDCVQTKGIECGDEYGEFVVTRAQN"
     gene            complement(3256..3792)
                     /locus_tag="PLQ54_02535"
     CDS             complement(3256..3792)
                     /locus_tag="PLQ54_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92164.1"
                     /translation="MAVRTKTEIVLEQRFDPRACRHSLNGRDVVLHCHHFVSLYTQLA
                     DDCALLDAKRLLAEVAEDAFGAALADYYAQHGINSIADRFDIATQYYAACGLGRMHVR
                     CAGPDSGSVELERSHVDEGWIAKWGQRPEPVNFITQGFIGGMFAAVMGRPPRSFRVSE
                     TASIVSGAERSLFEIVAQ"
     gene            complement(3818..4009)
                     /locus_tag="PLQ54_02540"
     CDS             complement(3818..4009)
                     /locus_tag="PLQ54_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92165.1"
                     /translation="MGDRLTKLYEYAEKEGGLQTKMRLAMKTGVPSAKAATEPDSPDL
                     IAKFKAAFKEITGKDAPPV"
     gene            complement(4074..4355)
                     /locus_tag="PLQ54_02545"
     CDS             complement(4074..4355)
                     /locus_tag="PLQ54_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92166.1"
                     /translation="MRIVATTPVQDCFDGSTVFTYWFDTVWTPDLVQVLRCLGELEYF
                     SDFPRPFFRLRCGSGLQVKGVVGEDNCQVILPRAEREVIRESFERCFAP"
     gene            complement(4346..>5385)
                     /locus_tag="PLQ54_02550"
     CDS             complement(4346..>5385)
                     /locus_tag="PLQ54_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOS92167.1"
                     /translation="GRTAAEDRSTLDECRDLIAEQLAVRDPHRIVLTPGSTYSINLAL
                     WGLGLRAAHRVVTTVTEHNSVLRPLYHLRERHGTSIAIVGLGADGALDMDRFKTALES
                     GATLVAANHASNVTGRVNDVACLFSMAHEAGAVTLLDASQSLGHVPVHPEELGADLVA
                     FTGHKGLLGPPGTGGLYVDPNLDLEPMLVGGTGVRSDLMLHPADMPTRLEAGTPNLPG
                     FAGLAAALRWTAGPERHGPAVASAMAERLRDGLRTLRGVRLVDDDSRAQRVPVVSFVV
                     DGWSVDEAGYALACSFGVQCRSGLHCAPLIHQALGTWPHGTVRLSPSAFTTTEEIARA
                     VEAVARLAGCA"
BASE COUNT          826 a         1721 c         1887 g          951 t
ORIGIN      
        1 ggtctcgatg atgaaccgcg cgggcccacg ggtgtggttg cccatcacca cggcggacac
       61 cacgccttcc cgcagccgga tgtggtcttt cgcctgctgg aacaggcagc ccgagatggt
      121 ggcgctgccg gagatcacgt cgatggcggg gacgggatcg gccccctcgg ccggcggccg
      181 gttcgcccac tggcagaagt tgcactgcga gagggatacc agcccgttgc cggcagcttc
      241 gacgatcttc tgatgcgggc cccagaagga gcagttggcc agctggacca cgccggagtt
      301 cgagggggcg atgcgcacca cggcctcgca ggccggcgag cccacgaact cgccattggt
      361 gatcaggagc ccgggcggct gcgtgctctc gaccagcagg gggatgacgg cccagtccgc
      421 cgcgatgccc aggaagttgc cgttggtggg cccggcctcg ctctcgaaga accggtagcc
      481 cacccggcag ccccaactga aggtgttgag catgaactcc cagtcgcttc ggccgatctc
      541 gaatgcaacc aagttggcct gcatgtactc gacgaggcgg gcgccgtcgt cgccctgggg
      601 cgccgtgtcc atccccgacc gggtccacga gttggggttg aagtgcacgt tctcgacgcg
      661 gccgatgtcc gtgcacttgt ccaggtgcac gccgatcttc agcgggcagc catagacatt
      721 ccggatgtag tgcatctcgt gggcgtaggt gccgaagtcg atgcccttgt aggggttgag
      781 cagcgtcacg tcggatacgt tcaggtgggt gccccgcccc tggatggtcc acgggtatgc
      841 agtgatttgc gacgccctct gctcggggta cacgacggtc aggccgcgca ccgtgctgtt
      901 ggtctcgagc atgagcaggg gaggtccatc ctcctgccca cggccctcgt aggcgagcag
      961 gaccgtgccg acctcggggg tggagaagtg cggggcttcc cacacacccg agagggtcac
     1021 gcccgtcggg atggtcaggt gcccgtcgag ccggtactta ccggagggca gggagaccac
     1081 gccgccgccg gcggcaccag cctggtccag ggcggcctgg atcgcggccg tgtcgtccgt
     1141 ggcgccatcc cccgccgccc cgaggtcctt ggcgttgatc tctcccgccg cggcaaccgc
     1201 ccccgcggcc agagccacac ttaccagtgc gccaaacagc ccgtgtagca gcatcaggta
     1261 gcctcccttc tggtgctggc gggtgtattc gtcgcgccgg tgtataatgc cctccccctg
     1321 gccacaaccc ggggagcgtc cgggcgccgc gcgaccgggt gtcatcggag tgtgagggtc
     1381 cgccacgatg aatgagatcg ccctgcgcta tgggtgcaat cccaatcaga agccggctcg
     1441 agcgtatgtg gagtcgggcg agatcccctt cgaggtgctc aacggggcgc caggctatat
     1501 caacctgctc gatgcgttga acgcgtggca gctggttcga gagcttcgcg atgcgctgga
     1561 tcggcccgcg gcggcttcgt tcaagcacgt cagtccggcg ggtgccgcgg tggccgtgcc
     1621 gctgagcgcc gagctgcgtc gggcgtacat ggtggatgat ctggatctct cgccgctggc
     1681 gacggcctat gctcgcgccc gcggtgccga tcagatgtcg tcctttggcg acttcgccgc
     1741 cctgagcgat gtggtcgacg tctctacggc caagctgctg cggcgcgagg tgtctgatgg
     1801 cgtcatcgcg ccgggctatg agcccgaggc gctcgaactg ctgaagcaga agaaggacgg
     1861 caagtacctg gtcatggcca tggacccggg gtatgtgccg ccagcgcagg aggtgcgcga
     1921 tgtgttcggc ctgcggctcg agcaggggcg caacgatgtg accatcgggc cagcgctcct
     1981 cgagcgcatc gtgacgcagc gaagggagct cccacccgaa gccgtgcggg atctgatcgt
     2041 ggccacggtg gcgctcaagt tcacgcagtc gaactcggtg ggcttcgcca aggacggcca
     2101 ggttatcggg atgggcgccg gccagcagtc gcgcatccat tgcacccggc tcgccggcga
     2161 gaaggcggat acctggtggc tccgacagca cccgaaggtc ctcgacttgc ccttccgccc
     2221 gggcgtgaag cggccggacc gtattaatgc catcgtgcag tatctgtggg ccgactgggc
     2281 gccggcggag cgacgggcgt gggaggcggt gttcgagccg gtgccggagc tgctcactga
     2341 ggccgagcgg cgggattggc tggatcagct gaccggggtg gcgttgagct cggatgcctt
     2401 cttcccgttc tcggactgca tctatcgggc ggctcgcagc ggggtgtcgt atgtggttga
     2461 gaccggcggg tcggtgcgtg atgatgcggt cattgccgcg gcggacgagt gtgggatggt
     2521 gatggccttc tcgggcgttc ggctgttcca ccattaggcg ccggtcttgt acggcgcgag
     2581 agcggccggt gcgggccgct ctcgtcggtt tgggtcgacg gtgggtgcga ctagttctgg
     2641 gctcgtgtca cgacgaactc gccgtactcg tcaccgcact cgataccctt ggtttgcacg
     2701 cagtcgaagg cccgcatggc ggctcctccg gcgggatcgt actcgccgcc ataggccagt
     2761 cccatgaacg cggcgcacac gccggtgatc atgtacgcgc ttggcttggt ggaccggcca
     2821 aaggtgacca cgtcggactc gtagtagtcg tatgcccgga ctaccatgcg ctcgttgggc
     2881 acgagctcga tgatctcgct cttggcccag ccccatgcgg tgaacacggc gaaggcaccg
     2941 tggaggatgt cctcgggcac tttttcgatc atgggcccga ccacggcttt ccattcctcg
     3001 gacgtgatga tgccgtagcc cgtgtggtag ccgcaggttc gcgccgcgtc gacgagtagc
     3061 cactcggcgg cctcgagcat ctcgggcccc acctggcggg tcaggcgctc ggcgaagctg
     3121 ttccagaagc tgcaaggtag ctggttcacg aacacgttga aggccgggat cagcccatag
     3181 tcatccgcac tcacctgcac cctggagagg gcgtcgatga ttcgcttgcg gtcgattgcc
     3241 accgtatgcc tccgttcatt gggcgacgat ctcgaagagg gatcgttccg cgccggacac
     3301 gatgctggcg gtctcactga cacggaacga gcggggcggc cgtcccatga cggcggcgaa
     3361 catccccccg atgaacccct gggtgatgaa gttgactggt tcggggcgct ggccccactt
     3421 ggcgatccag ccctcatcga catgggagcg ttcgagctcg accgaccccg aatcggggcc
     3481 cgcgcatcgg acatgcatcc tgcccagccc gcaggcggcg tagtactggg tggcaatgtc
     3541 aaatcggtca gcgatcgagt tgataccatg ctgggcgtag tagtcggcca gggctgcgcc
     3601 gaaggcgtct tcggccacct cggccaggag tctcttggcg tcgaggaggg cgcagtcgtc
     3661 ggccagctgc gtgtagagcg agacgaagtg gtggcagtgc aggacgacgt cccgcccgtt
     3721 caggctgtgc ctgcatgccc tgggatcgaa gcgttgctcg agcacgatct cggtctttgt
     3781 gcgaacagcc atcgctctgt ctccaatccg aatcgcccta gaccgggggc gcgtccttgc
     3841 ccgtgatctc tttgaacgcg gctttgaact tggcgatcag gtcgggcgag tctggctcgg
     3901 tggcggcctt ggcggacggg acgccggtct tcatggcgag ccgcattttg gtctggaggc
     3961 cgccttcctt ctccgcgtac tcgtacagct ttgtcagtct gtcccccatg ggaactcctt
     4021 ccgcgttgag actgccccag cgctgcgtcc ggtgtgcgca gccgtgaccc tctctatggg
     4081 gcgaaacacc gctcgaagct ctctcggatc acctcccgct ccgcccgggg gagtatgacc
     4141 tggcagttgt cctcccccac cacgcccttg acctggagcc ccgaaccgca gcgcagccgg
     4201 aagaatgggc gtgggaagtc actgaagtac tccagctcgc ccaggcaccg tagcacctga
     4261 acgagatcgg gcgtccagac ggtgtcgaac cagtatgtga acacggtgct gccgtcgaag
     4321 cagtcctgca cgggagtggt cgctactatg cgcatccggc cagcctcgca acggcctcga
     4381 cggccctagc gatctcttcg gtggtggtga acgccgatgg gctgagtctc acggtgccgt
     4441 ggggccaggt gcccagggcc tggtgtatca gcggggcgca gtggagtccg gaacggcact
     4501 gcacaccgaa gctacaggcc agcgcgtagc cggcctcgtc cacgctccag ccgtcgacga
     4561 cgaaggagac gacggggacc cgctgggccc gggagtcgtc atcgaccagt cttacgccgc
     4621 ggagtgtccg cagcccatcg cggagccgct cggccatggc gctggcgacg gcgggcccgt
     4681 ggcgttcggg ccctgccgtc cagcgcagcg ctgctgccag tccggcgaac ccgggcaggt
     4741 tgggcgtgcc ggcctcgagg cgtgtgggca tgtcggccgg gtggagcatc aggtcgctgc
     4801 ggacaccggt cccgcccacc agcatcggct cgaggtcgag gttcgggtcg acgtagaggc
     4861 cccccgtgcc cggggggccg agaaggccct tgtgccccgt gaacgccacc aggtccgcac
     4921 ccagctcctc ggggtggacg ggcacgtgac ccagcgactg ggaggcatcc agcagggtca
     4981 ccgcaccggc ttcatgggcc attgagaaga ggcaggccac atcgttgacg cgaccggtga
     5041 cgttggatgc atgattggcg gccacgagcg tagcgcccga ttccagggca gtcttgaatc
     5101 gatccatgtc cagcgccccg tcggccccaa ggccgacgat ggcgatggag gtcccgtgcc
     5161 tctccctcag gtggtagagc ggacgcagca ctgaattgtg ctccgtgacc gtggttacga
     5221 ctcggtgggc cgcgcggagt cccaggcccc agagcgcgag gttgatcgag taggtcgagc
     5281 cgggcgtcag gacgatgcgg tgggggtcgc gtacggccag ctgttctgcg atgaggtctc
     5341 ggcactcgtc gagtgtgctc cggtcctccg ccgcggtgcg gccgg
//
LOCUS       DAOSPL010000085         5374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_15360_length_5374_cov_2.838127, whole genome shotgun sequence.
ACCESSION   DAOSPL010000085 DAOSPL010000000
VERSION     DAOSPL010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15360_length_5374_cov_2.838127"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PLQ54_02555"
     CDS             <1..613
                     /locus_tag="PLQ54_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92168.1"
                     /translation="VKAIATHADLPAIGDKMSDGGEGAVNLRHMSSNILAFGKVLYWG
                     HAIAAVAATTQTIADQAIALIKVEYEVLPPVLDLKTGTMPTGSLQLKTYDLGMKRNYG
                     ISADWGMFWTPGGGEDGGKLSQAVDLKAWSDDRVEEMYASAMRGIKAGVFLPHVTAMC
                     RGCTVRDFCWAVAGERAEELPAIPVVIDRASGEISAPSADAQV"
     gene            666..914
                     /locus_tag="PLQ54_02560"
     CDS             666..914
                     /locus_tag="PLQ54_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92169.1"
                     /translation="MAIKHAGSVLLSLTYEESMILESLLYSVESDEPKITDAIAAMHN
                     ALAGDAEWVYRCADKIVRPADGGSLNVVAGEDMDEVLW"
     gene            911..1369
                     /locus_tag="PLQ54_02565"
     CDS             911..1369
                     /locus_tag="PLQ54_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92170.1"
                     /translation="MSGPDDRSTATIKFGKDFEAPWYVASGNVVTQRQQLIEFAGMED
                     NPELSLLDLMVLVASEAQAKWAIRSKLGAQPSSTEIDVPKKSQPATEKVASKPAEERA
                     EADPSAFIFELIKEASDLKALAKVFLANKDAFNTNKALQGAFNARKAELS"
     gene            1387..1833
                     /locus_tag="PLQ54_02570"
     CDS             1387..1833
                     /locus_tag="PLQ54_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92171.1"
                     /translation="MEFITTTSGGGGYFKPEEHADAKALLIEPTRYLPEEPNPFGKPG
                     ETREILVADVTVFVNKDALEGEVEPVVLQSTRLTAGALVKEYKDKIGKAAICSLQLKP
                     ATMPGRKAFWVPKAVDKAAAELVIAYAKEREAEAEAVMDELPDWAA"
     gene            1833..2429
                     /locus_tag="PLQ54_02575"
     CDS             1833..2429
                     /locus_tag="PLQ54_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92172.1"
                     /translation="MRIEQPLRIFTYHPTAGDLTIGAPMNDNFYNAITINDLEDGALV
                     IGVYSHNLAVLNEDASRARYLGALGTLSPPVTAYPDEYLATEDPDIERLGFEPVSTGP
                     TKLYLLKDSDILGLEYGDEVYAFDTKPDADREIRVHKADDPHDDAYVKAEHLSFAKPV
                     KRYPADVIRKAFEMFGSDERQISQVFLILDGMVAQKAA"
     gene            2434..3243
                     /locus_tag="PLQ54_02580"
     CDS             2434..3243
                     /locus_tag="PLQ54_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS92173.1"
                     /translation="MLTATRAFNLNARSAPLLPQVEELAPLYQWGVSPRQGQVIMIAG
                     RSGSQKSGFAIFWTRKMRLPTLYMSGDMTAFEATSRLVGMETGEGIEAIEAGLEGPDR
                     LRYEQSLEDVNITFSFGQPITFPGIEAELEAWVELHNCYPAIIVIDNLMDIDGCEADY
                     SAQQEAMQILSGLARETGSTIIVIHHATDKSYEANSQPGYPPSRKEIKNGLSEKPQLT
                     LTVALEPEYGELRIACVKQRSGRSDPSASDFIRLRAYPEITRFGPLRSSYH"
     gene            3256..3393
                     /locus_tag="PLQ54_02585"
     CDS             3256..3393
                     /locus_tag="PLQ54_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92174.1"
                     /translation="MSFHGAQHKGAARDRKELKQTQAEARNAETLPERRRAARLEVAD
                     D"
     gene            3386..3610
                     /locus_tag="PLQ54_02590"
     CDS             3386..3610
                     /locus_tag="PLQ54_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92175.1"
                     /translation="MTEFKIVVKCATDLEPLIGQEVDIKPLGTLDWLIPGLVVRDLGQ
                     RWRVGFVDCRDHTVKAFLPGDTLTPRGGTS"
     gene            3607..3816
                     /locus_tag="PLQ54_02595"
     CDS             3607..3816
                     /locus_tag="PLQ54_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92176.1"
                     /translation="MTDSDCREAAMRCIVEMVEAIAEQVSGQDLVGHTSLLAGQSRAV
                     HERVVELIDTALVTVTVDWEGGIHA"
     gene            3813..4331
                     /locus_tag="PLQ54_02600"
     CDS             3813..4331
                     /locus_tag="PLQ54_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92177.1"
                     /translation="MKVIGIDPSLSSTGLCWAIGESETIKLKADDPRRLVRLHDIVYS
                     RAVAQRLAVIEDLPTHAMSAGLTGQAQGLVRKVLQEQEIPYLTVPPATLKKFATGSGK
                     ATKGEMLKAYEARMGTAPGGMDDNQIDAWWLREMGVHYLRHFDTVRRSHFDPSGTCKW
                     SDIPWTEGVPTP"
     gene            4328..>5374
                     /locus_tag="PLQ54_02605"
     CDS             4328..>5374
                     /locus_tag="PLQ54_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOS92178.1"
                     /translation="MSKFDSVDVVDLFAGPGGWDVGLRMIDPHLSVLGVEWDEAACQT
                     AEAAGFPRLCGDVRKFDSRQFPNAKGLIASPPCPTFSAAGNGSGRRQLNLVIDAMETL
                     WRGEDVDCDHFEDERTALVLEPMRWVLEAWHAGHAYRWIALEQVPGALPAWEAMVRYL
                     KHLGYSVATGILNAEQYGVPQTRKRAVLIASLDHEVQLPTPTHSRYYPRAPHKLDPGV
                     KKWVSMAEALGWGMAERPSMTVTGGGAETGGAEPFGTGARRGMLRAQASGSWEFVMMS
                     NYGTGGDASKRGLRSSDQPAPTITSKVDRNKVYLVDDGNFAITGQARNSGPGASRRPH
                     GVDDPSYTIRANGSG"
BASE COUNT         1032 a         1749 c         1738 g          855 t
ORIGIN      
        1 ggtcaaggcg atcgccaccc acgcggactt gccggccatc ggcgacaaaa tgtccgacgg
       61 cggcgagggc gcggtcaacc tgcgccacat gtcaagcaat atcctggcct ttggcaaggt
      121 cctttactgg gggcacgcga ttgcggccgt ggcggcgacg acccagacga ttgccgacca
      181 ggcgattgcc ctgatcaagg tcgagtacga agtcctgccg cccgtgctcg acctgaagac
      241 cggcaccatg ccgaccggct cgctccagct caagacctac gacctgggca tgaagcgcaa
      301 ctatgggatc tccgccgact ggggcatgtt ctggacaccg ggcgggggcg aggacggtgg
      361 caagctatcc caggccgtcg acctcaaggc ttggtctgat gaccgagtcg aggagatgta
      421 cgccagcgcg atgcgcggca tcaaggcagg cgtgttcctg cctcacgtca cggccatgtg
      481 ccgtggctgc accgtccgag acttctgctg ggcggtggct ggggagcggg ctgaggagtt
      541 gcccgcgatt cccgtcgtca tcgatcgcgc ttcgggagag atctccgctc cttccgctga
      601 tgcgcaagtg tgactaagtc gcgccaccgt ggcgcaaaac tcgcaagaat cttgaggagg
      661 attcaatggc aatcaagcac gccggcagcg ttctgctgtc cctgacctac gaagagtcga
      721 tgatcctgga gtcgctgctc tacagcgtgg agagcgatga gccgaagatc acggacgcga
      781 tcgccgccat gcataacgcg ctcgctggcg acgccgaatg ggtgtaccgc tgcgcggaca
      841 agatcgtccg ccccgccgat ggaggctccc tgaacgtcgt ggctggcgag gacatggacg
      901 aggtcctgtg gtgagcggcc ccgacgatcg cagcaccgcg acgatcaagt tcggcaagga
      961 cttcgaggcg ccctggtacg tggccagcgg caacgtggtc acccagcgcc agcaactcat
     1021 tgagttcgcc ggcatggagg acaacccgga gttgagcctg ctcgacctga tggtgctcgt
     1081 cgcgagcgag gctcaggcga agtgggcgat ccgctccaag ctcggagccc agcccagctc
     1141 gaccgagatc gacgtcccga agaagtctca gcccgcaacc gagaaggtgg cgagcaagcc
     1201 cgccgaggag cgggccgagg ccgatccgag cgccttcatc ttcgagctga tcaaggaggc
     1261 ttccgacctc aaggccctgg cgaaggtctt cctcgccaac aaggacgcct tcaacaccaa
     1321 caaggccctt cagggcgcat tcaacgctcg caaggccgag ctttcctaat acgaggagat
     1381 cactacatgg agttcatcac caccaccagc ggcggcggcg gctacttcaa gccggaggag
     1441 cacgccgacg cgaaggccct gctgatcgag ccgacccgct acctgcccga ggagccgaat
     1501 ccgttcggca agcccggcga aacccgcgag atcctcgtcg ccgacgtgac ggtcttcgtc
     1561 aacaaggacg ccctggaggg cgaggttgag ccggtcgtct tgcagtcgac ccgcctcacc
     1621 gctggcgctc tcgtcaagga gtacaaggac aagatcggca aggccgcgat ctgctcgttg
     1681 cagctcaagc cggcgaccat gcccggacgc aaggcgttct gggtgccgaa ggctgtcgac
     1741 aaggcggctg ccgagctggt catcgcctac gccaaggagc gcgaggccga ggcggaggcc
     1801 gtcatggacg agctgcccga ctgggcggcg tagtgcggat cgagcagccg ctgcggatct
     1861 tcacctacca ccccaccgcc ggagacctca cgatcggagc acccatgaat gacaacttct
     1921 acaacgccat cacgatcaac gacctggagg acggcgcgct cgtcattggc gtctacagtc
     1981 ataatctggc cgtcctgaat gaggatgcca gtcgcgctcg ctacctcgga gcactcggca
     2041 ccctgagccc ccctgtcaca gcctacccag acgaatatct cgccaccgag gacccggaca
     2101 tcgagcgcct gggcttcgag cctgtctcga ccggcccgac caagctgtac ctgctcaagg
     2161 attccgatat cctcggcctg gagtacggcg acgaggttta cgccttcgac accaagccgg
     2221 acgccgatcg cgagatcagg gtccacaagg ccgacgatcc gcacgacgac gcctacgtca
     2281 aggcggagca cctgagcttc gcaaagccgg tcaagcgcta cccagccgac gtgatccgca
     2341 aggcgttcga gatgttcggt tcggatgagc gtcagatctc gcaggtcttc ctcatcctcg
     2401 atggcatggt cgcccagaag gcggcttgat cgagtgctga ccgcgacgag ggcattcaac
     2461 ctgaacgctc ggtcggcacc cctcctccca caggtcgagg agctggcccc cctgtaccag
     2521 tggggggtca gtcctcgcca gggtcaggtc attatgatcg ctggccgcag cgggtctcag
     2581 aagtctggct ttgccatctt ctggactcgc aagatgcgcc tgccaacgct ctacatgagc
     2641 ggcgacatga ccgccttcga ggcaacctcg cgcctggtcg gcatggaaac aggcgagggg
     2701 atcgaagcta ttgaggcggg gttggagggt cctgaccgac tccgctacga gcagtccctt
     2761 gaggacgtga acatcacctt cagcttcggg cagccgatta ccttcccggg gatcgaggcc
     2821 gagctggagg cgtgggtcga gctacacaac tgctacccgg cgatcatcgt catcgacaac
     2881 ctgatggaca tcgacgggtg tgaggctgac tactcggcac aacaggaggc catgcagatc
     2941 ctgtccggcc tggcgcgcga gacgggctcc acgatcatcg tgatccatca cgcaaccgac
     3001 aagtcctacg aggcgaactc ccagcccggc tacccgccgt cgcgtaagga gatcaagaac
     3061 ggcctgagtg agaagcccca gctcacgcta acggtcgccc tggagcccga gtacggcgag
     3121 ctgaggatcg cctgcgtgaa gcagcgctct ggccgcagcg acccatcggc ctcggacttc
     3181 atccgcctcc gggcctaccc ggagatcaca cgcttcggcc cactgaggtc gagctaccac
     3241 taacgaggag tgaccatgag ctttcatgga gcgcagcaca agggagcggc ccgagaccgc
     3301 aaggagctga agcaaaccca ggccgaagct cggaacgcag agaccctgcc tgagcgccgt
     3361 cgagcggcac ggctggaggt cgctgatgac tgagttcaag atcgtggtca agtgcgccac
     3421 agacttggag cccttgattg ggcaggaggt cgacatcaag cccctgggca cgctggactg
     3481 gctgatccct ggcctcgtgg tgcgcgacct tggtcagcgg tggcgtgtcg gattcgtgga
     3541 ctgccgcgac catacggtca aggcgttcct ccccggcgac accctgaccc cgaggggggg
     3601 cacgtcgtga ccgacagcga ctgccgtgag gcggcgatgc gctgcatcgt cgagatggtt
     3661 gaggccatcg ccgaacaggt gagcggccag gacctcgtcg gtcacacgag cctcctcgcc
     3721 ggccagtcgc gggcagtcca cgagcgtgtc gtcgagctga tcgacacggc cctcgtgacc
     3781 gtgacggttg actgggaagg gggtatccac gcatgaaggt gatcggaatc gatccgtccc
     3841 tgtcctcaac cggtctgtgc tgggcgatcg gcgagtctga gaccatcaag ctcaaggccg
     3901 acgacccaag gcgacttgtc aggctgcacg acatcgtcta ctcgcgagcc gtcgcccaga
     3961 ggctggctgt gatcgaggac ctccccaccc acgccatgtc cgcagggctt actgggcaag
     4021 cgcagggctt ggtacgcaag gtcctccagg agcaggagat tccctacctg acggtccccc
     4081 cggcgacgct caagaagttc gcgacgggga gcgggaaggc gaccaagggc gagatgctca
     4141 aggcgtacga ggctcgcatg ggcaccgccc cggggggcat ggacgacaac cagatcgacg
     4201 cctggtggct tcgtgagatg ggcgtccatt acctccgcca cttcgacacc gttcgccgat
     4261 cgcacttcga tccgagcggc acctgcaagt ggtccgacat tccctggacg gaaggagtcc
     4321 cgacaccatg agtaagtttg actcagtcga tgtggtcgac ttgttcgcag gccccggcgg
     4381 ctgggacgtc gggctgcgga tgatcgaccc acatctgagc gtgctgggtg tcgagtggga
     4441 tgaggccgcc tgccagaccg ccgaggctgc gggattcccg cgcctatgtg gcgacgtgcg
     4501 caagtttgac tcccgccagt tcccgaacgc caagggcctg attgctagcc cgccctgtcc
     4561 gacattctcc gcagccggca acggctcggg tcgccgccag ctcaacctcg tcatcgacgc
     4621 gatggagacc ctctggcgtg gtgaggacgt ggactgtgac cacttcgagg acgagcgaac
     4681 agccctcgtc ctggagccga tgaggtgggt gctcgaagcc tggcacgcgg gtcacgccta
     4741 ccgctggatc gccctggagc aggtgcctgg cgcgctcccc gcgtgggagg cgatggtcag
     4801 gtacctgaag cacctcggct acagcgtcgc cacgggcatc ctcaacgccg agcagtacgg
     4861 ggtgccccag acccgcaagc gcgcggtgct gatcgccagc ctggaccacg aggtgcaact
     4921 gcccacccca acccactccc ggtactaccc ccgggcgccg cacaagctgg accccggcgt
     4981 gaagaagtgg gtcagcatgg ccgaggcgct ggggtggggc atggccgagc gtccctccat
     5041 gaccgttaca gggggtggtg ctgagaccgg gggtgcagag cccttcggca ccggggcccg
     5101 aaggggaatg ctccgagccc aggcttcggg aagctgggag ttcgtgatga tgtctaacta
     5161 cggcaccgga ggggacgcct ccaagcgcgg cctccgaagt tcggaccagc ccgcgccgac
     5221 gatcacctcg aaggttgacc gcaacaaggt gtacctcgtg gacgacggca acttcgcgat
     5281 caccggccag gcccgcaact cggggccagg cgcttcgcgg cgtcctcatg gagttgacga
     5341 tccctcctac acaatccgag ccaacggctc cggc
//
LOCUS       DAOSPL010000086         5347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_15458_length_5347_cov_3.455404, whole genome shotgun sequence.
ACCESSION   DAOSPL010000086 DAOSPL010000000
VERSION     DAOSPL010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15458_length_5347_cov_3.455404"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1670
                     /gene="polA"
                     /locus_tag="PLQ54_02610"
     CDS             <1..1670
                     /gene="polA"
                     /locus_tag="PLQ54_02610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOS92179.1"
                     /translation="LHAALRGTALVGLGLSVGGERTWHFDLGATVQPGLFDEPAAVEL
                     PADLAGLLADAGVAKCCYDAKSLERALAAMGVALAGVRFDALLAGYLLSFGEGSLGLS
                     RLAQRYLERPFEDSSGPEGLRAGALAVDQLERPLREGLEREGLTALLDTVELPLSHIL
                     AKMELTGVCVDRAALRDLSEKLAAEIRLAEEKVFEHAGERFVIGSPKQLQYILFEKLK
                     LPQGRKTKTGFSTDADVLSALAEQHEIVRHILDYRTYAKLKSTYADALADLADPETGR
                     VHTRFNQATVVTGRLSSSEPNLQNIPVRTEWGQKVRRCFVAPDPGTVLLACDYSQIEL
                     RVLAHFSKDEALCSAFGHGEDIHTRTAANVFGVELADVTQAMRGQAKTVNFGIIYGMG
                     IQALARDLGVSKDEAADFMERYFRTYAGVKAFIDETIARAKELGYVTTLLGRKRTLPE
                     LASPRVEVRRFGERTAVNTPIQGTAADMIKKAMIDVDAALAKWPEVRMILQVHDELLF
                     ELPERLVNEVAGEVCRLMAGTMPLDVPVVVDAKVGPNWGEMEPLQLA"
     gene            1804..3306
                     /locus_tag="PLQ54_02615"
     CDS             1804..3306
                     /locus_tag="PLQ54_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92180.1"
                     /translation="MSWWRSFLVHLLLAPIMPVLGWGFVTHALINRRAWRKAKASGKL
                     PNVLASDAARHRFEAAGSLPDTISLYTLTSRDRTFDPLHNMHMVGGKRVPLFGLALLD
                     AALEHWGPTVAVSAYGWCAHQLADSTAHYKGGFCTSLPTFRPAMGSYRPGAAGTARRR
                     AADKVLADVDHGLVELLADAASAAAGHADPPLGDPDIDLPPDAVQQAWDAVGYAPIGI
                     GQKWEALEAEFRSMLAAEVGLARALSRYEPGEQPVASAMETYYRDDGALKGYTSAVDA
                     AVDLVADLLSTDQQVLRQEGRGRRLVPAAPAGELPVIGLPTGSGTLQYRLFAALSPHL
                     NLLGARAPGVVLNGARGLLRASDATLVYRALLPFVETLRDGRDIATAAISAVRALPPI
                     ADPNGTLPKPGTETAPTGSLELLLLDFDEWSDTATQGSDLGISAVMLDGYPVHPPPQV
                     VVEWRQPVRGWHVAVDLGQLLQPGRHVVRVEGRTSDERPLRYEWSFTVSE"
     gene            3333..4163
                     /locus_tag="PLQ54_02620"
     CDS             3333..4163
                     /locus_tag="PLQ54_02620"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOS92181.1"
                     /translation="MSAAVRLVVLDVEGVITRAGGSHEPWPLEEIVALRDLIAGTPLA
                     VVLCTGRQVPYGEAIVQALGLLRPLPASIRQRVAARGLGFAGWPSVLENGAYLYDALG
                     RCAIALPSAHPGALEDLPRLRRDLLAPLVEETGATFEPGKDVCISINPPPVRPGAIER
                     VPTDEFRPVVDERLRGHDSLIEVSHSKSAIDITPKGISKASGVGQLLDCLGLGPDEVL
                     GVGDTRADAAWLSMLRWQATPSNGREALPHVAYHAPHPDVGGLIDILKRLVLRGYEGV
                     "
     gene            complement(4175..4819)
                     /locus_tag="PLQ54_02625"
     CDS             complement(4175..4819)
                     /locus_tag="PLQ54_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92182.1"
                     /translation="MSEDTEARPTTPEEVSEPSSPLHSPSVDTDVSASRGWPAAAIWT
                     TIVLAVLCLCAVVVLVLGILGSKQSDEAQGGLAGVFRRAYGEPSSSREARGQLTELAR
                     KDLTTALEHADNGDFSLARQATERAKTLLGVVKTLKPKDTPPEVAQVDAVLENLEKRD
                     FEGYAAAKKQLGELLGQGQPAKEDASSTAESAASASPAQPAAADADKAARAAGQ"
     gene            complement(4862..>5347)
                     /locus_tag="PLQ54_02630"
     CDS             complement(4862..>5347)
                     /locus_tag="PLQ54_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92183.1"
                     /translation="LGPGADVFDNALPSGSAVALHALIRAASLTGNMELLGLVRSAFE
                     AHAAAMSAHGMDMAWWLDGVELLLGPPLLIVVAGDGSDPRTRELLEAYRGTRAPHACL
                     VRVPPEGPDEAALTLFPPVAGLRAAACDSVARVCEPGQCRESARDGEGLRRLLTEGWH
                     C"
BASE COUNT          837 a         1775 c         1873 g          862 t
ORIGIN      
        1 cacttcacgc agcgctgcgc gggaccgctc tggtgggcct cggcctctcg gtgggaggcg
       61 agcggacatg gcacttcgac ctcggtgcca ccgtccagcc cgggctgttc gacgagccgg
      121 cggccgttga gctgcccgcc gacttggctg gcctgttggc cgacgccggc gtggcgaagt
      181 gctgctacga cgccaagtcg ctcgaacggg cgctggccgc catgggcgta gccctggccg
      241 gggtgcggtt tgacgcgctt ctggcgggct acctgttgag cttcggcgag ggttcgctgg
      301 gcctgagccg gttggcacag cgctacctgg agagaccctt cgaagactcg tcgggcccgg
      361 agggccttcg ggctggcgcg ttggccgtgg accaactcga gcggcccctt cgcgaggggc
      421 tcgagcgtga ggggctcacc gccctgctcg acaccgtcga gctcccgttg agccacatcc
      481 tcgcgaagat ggagctgacc ggcgtctgcg tcgaccgcgc ggcattgcga gacctgagcg
      541 agaagctcgc ggccgagatc cggctggcgg aggagaaggt cttcgagcac gccggcgagc
      601 ggttcgtcat cggcagcccg aagcagcttc agtacatcct cttcgaaaag ctcaagttgc
      661 ctcaggggcg caagaccaag acagggttct cgaccgatgc cgatgtgcta tcggccttgg
      721 ccgaacagca cgagatcgtg cggcacatcc tcgactaccg cacctacgcg aagctcaagt
      781 cgacctacgc cgatgccctg gccgacctgg ccgatcccga gaccgggcgc gtgcacacac
      841 ggttcaacca ggccacggtg gtcacgggcc gtctctcgtc gagcgagccg aacctgcaga
      901 acatcccggt ccgcaccgag tggggccaga aggtccgccg gtgcttcgtg gcacccgacc
      961 cgggcaccgt gctgctggcg tgcgactaca gccagatcga gctgcgtgtt ctcgcccatt
     1021 tctcgaagga cgaggccctc tgcagcgcct tcggccacgg cgaggacatc cacactcgca
     1081 cggcggcgaa cgtcttcgga gtcgagctgg cggacgttac ccaggccatg cgtggtcagg
     1141 cgaagacggt gaacttcggc atcatctacg gcatgggcat ccaggcgctg gcccgcgacc
     1201 tgggcgtatc gaaggacgag gccgccgact tcatggagcg gtacttccgg acgtatgccg
     1261 gcgtgaaggc cttcattgat gagaccatcg cgcgggccaa ggagctggga tacgtcacca
     1321 cgctgctggg tcgcaagcgg acgcttccgg agctggcgag cccgcgggtg gaggtacgcc
     1381 ggttcggaga gcggacggcg gtgaacacgc cgatccaggg gacggcggcc gacatgatca
     1441 agaaggcgat gatcgatgtc gacgcggcgc tggcgaagtg gcccgaagtg cggatgatcc
     1501 tccaggtgca cgacgagctg ctgttcgagc tgccggagcg cctcgtcaat gaggtggccg
     1561 gcgaggtctg ccggctcatg gccgggacca tgccgctcga cgtgcccgtg gtggtcgatg
     1621 ccaaggtcgg gccaaactgg ggcgagatgg agccgctgca gctggcgtag gccgttcatt
     1681 ccggtgcagc aaaggagtac tactatccaa agcgaaccgg gccgctaaca tttatgaagc
     1741 tgtactactc actgcgctag atcggtctgg gagacggtgc ggcgttccgg aggtgctccg
     1801 ctgatgagct ggtggcgctc gttcttggtc catctcctcc tcgccccgat catgcccgtc
     1861 ctcggctggg gctttgtgac ccacgccctc atcaaccgcc gtgcgtggcg gaaggcgaaa
     1921 gccagcggca agctaccgaa cgtgttagcc agcgacgccg cgcgacaccg tttcgaggcc
     1981 gcgggatcgc tgcccgacac catctcgctc tacacgctca cgtctaggga tcgcaccttc
     2041 gatccacttc acaacatgca catggtgggg gggaagaggg tcccgctgtt cggtctggcc
     2101 ctcctcgacg cggcactcga gcactggggt ccgaccgtgg ccgtgagtgc gtacggctgg
     2161 tgtgcgcatc agctggcgga ctcgacggcg cactacaagg gcggcttctg cacctctctg
     2221 cccacattcc gacccgccat gggcagctac cggcctggcg ctgccggcac cgcccggcgc
     2281 cgtgccgccg acaaggttct cgccgacgtg gatcacggct tggtcgagct gctcgcggac
     2341 gcggcctcag cggccgccgg tcacgcggat cctccgctcg gcgaccctga tatcgacctg
     2401 ccgcccgacg cggtgcagca ggcctgggac gctgtgggct atgcgcccat cggcatcggc
     2461 caaaagtggg aagcgctcga agcggagttc cggagcatgc tggctgccga ggtcgggctg
     2521 gcacgggccc tatcgcggta cgagccaggc gagcagccag tggccagcgc catggagacc
     2581 tactaccgcg acgacggcgc cctgaagggc tacacgtcgg ccgtggacgc ggcggtggac
     2641 ctcgtcgcgg acttgctatc caccgatcaa caagtgctca ggcaggaggg ccggggccgt
     2701 cggcttgttc cggcggcgcc cgccggcgag ctcccggtca tcgggttgcc cactggcagc
     2761 ggtacgttgc agtaccggct gtttgcggcc ctatcgccgc acctcaacct cctgggagcg
     2821 cgggctcctg gagtggtact gaacggggcg cgcgggttgc tgcgcgccag cgacgccacg
     2881 ttggtctatc gggccctgct gccgttcgtc gaaacgctgc gcgatggccg cgatatcgcg
     2941 accgcggcga tcagcgcggt gagagcactg cccccgatcg cggacccgaa cgggaccttg
     3001 ccgaaacccg gcacggagac agcgccgacg gggtcactgg agcttctcct actggacttc
     3061 gatgaatgga gtgacacggc gactcagggc agcgacctcg ggatctcggc ggtgatgctg
     3121 gacggctacc cggtgcaccc gccgcctcag gtagtggtcg agtggcgcca gccggttcgc
     3181 ggatggcacg tcgctgttga tctgggccag ttgctccaac cagggcggca tgtggtccgt
     3241 gttgagggcc gcacctcgga cgagaggccc ttgcgctatg agtggagctt cacggtgagc
     3301 gagtaggggt ctcgctcgcg gcgagaggaa gggtgagcgc agcggtccgt ctggtggttc
     3361 tcgatgtcga gggcgtcatc acccgcgcgg gtggctcgca cgagccgtgg cccctcgagg
     3421 agattgtcgc ccttcgcgac ctgatcgcgg gcacgccgct ggccgtggtg ctgtgcacgg
     3481 gccggcaggt cccgtatggc gaagcgatcg ttcaggctct ggggctgctc cgtccgctac
     3541 ccgcgagcat tcgtcaacgc gtggcagcgc gaggcctcgg cttcgccgga tggccctcgg
     3601 tgttggagaa cggcgcctac ctgtacgatg cattggggcg gtgtgccatc gctcttccct
     3661 cggcccatcc tggtgccctc gaggacctgc ctcgcttgcg gcgagatctc ctcgcgccac
     3721 tggtggagga gacgggagcc accttcgagc cgggcaagga tgtgtgcatc tccatcaacc
     3781 cgccgccggt gaggccgggc gccattgagc gggtgccgac cgacgagttc cgtcccgtcg
     3841 tggacgagag gttgcggggg cacgattcgc tgatcgaggt gagccacagc aagagtgcca
     3901 tcgacatcac acccaagggc attagcaagg cgtccggagt cggccagctg ctggactgcc
     3961 tcggccttgg gcccgacgaa gtgctggggg tcggggacac ccgggccgat gccgcctggc
     4021 tgtcgatgct gcgctggcag gccacgcctt cgaacggccg cgaagcgctc ccccatgtgg
     4081 cgtaccacgc gccccacccg gatgtggggg gcctgatcga catactgaag cgcctggtgc
     4141 tgcggggcta tgagggcgtg tgagcgcctc tgcgctactg cccagcggca cgcgccgcct
     4201 tgtccgcatc ggctgcggct ggttgggccg gcgacgccga ggcggccgac tcagcggtac
     4261 tggacgcatc ctctttggca ggctgcccct ggcccaacag ctcgccgagc tgcttcttcg
     4321 cggcagcgta gccctcaaag tctcgcttct cgaggttctc gagcacggcg tcgacctgag
     4381 cgacctcggg cggcgtgtcc ttgggcttga gggtcttcac gacaccgagc agggtcttgg
     4441 cccgctccgt ggcctgccgc gccaggctga agtcgccgtt gtcggcatgc tcgagcgcgg
     4501 tggtgaggtc cttccgagct agctcggtga gttgtccgcg ggcctcgcgc gaggagctgg
     4561 gctcaccgta ggcgcggcgg aaaacgccgg ccaggccccc ctgcgcctca tccgactgct
     4621 tcgagccgag gatgccgaga accagcacga cgacagcgca gaggcacagg acggcgagga
     4681 cgatcgtcgt ccagatcgcg gccgcaggcc aaccccgcga cgcggacacg tctgtatcga
     4741 ctgagggact gtgcagcggc gaactgggct cagagacttc ctcaggcgtg gtgggccgag
     4801 cctcggtatc ttcgctcatg tcaagccgtc ctttccagtg cgcgccgagc ctgtggcccg
     4861 gctagcagtg ccaaccctcg gtaagcagac gccgaaggcc ttcgccatcc cgggcggatt
     4921 ccctgcattg cccgggctcg catacccgag cgaccgaatc gcaggctgcg gcccggagac
     4981 ccgcgacggg cgggaacagc gtcagggcag cctcgtccgg cccttcaggc gggacgcgaa
     5041 ccaggcatgc gtgcggcgca cgcgtgccac ggtacgcctc aaggagctcc cgagtccgtg
     5101 ggtcggaccc atcgccggcc accacgatga gcagcggcgg acccagcagg agctcgacgc
     5161 cgtccaacca ccaggccatg tccatcccat gggcagacat ggccgcggcg tgggcctcga
     5221 acgcagaccg aaccagcccg agcagctcca tgttgcccgt cagcgaggcc gcacggatca
     5281 gggcatggag cgccacggcc gagccactcg ggagtgcgtt gtcaaacacg tcggccccag
     5341 ggccaag
//
LOCUS       DAOSPL010000087         5338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_15492_length_5338_cov_2.737270, whole genome shotgun sequence.
ACCESSION   DAOSPL010000087 DAOSPL010000000
VERSION     DAOSPL010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15492_length_5338_cov_2.737270"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PLQ54_02635"
     CDS             complement(<1..436)
                     /locus_tag="PLQ54_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92184.1"
                     /translation="MVRKRVPIVALLVVISLAVLAGCQRISDRLASGDEPSTPSAPVM
                     ESTRPPVDLEAEPPSAPPEAETGEKTSADEAGATQGTTGAGESTKGAADASSQPAEEA
                     KSPEALSQAAKGSGKTAPAGASAGSRTTASGPSERDQLLAQLR"
     gene            705..1493
                     /locus_tag="PLQ54_02640"
     CDS             705..1493
                     /locus_tag="PLQ54_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92185.1"
                     /translation="MVHPTRFPGSPCLTAWLAALAVLLAGCGGSPIDVTPGGNGNSNN
                     NGGTAAFGEGDAFYITVPAPSGTGNAYLPGPGLSSPSGSANDPPLPPGQDPTSGEGVV
                     GVFLEGLDGTLTGSSFDVARSEADVNACGGGCLLASRSATSGNAVVRVILANATGTVW
                     DVTALGTVSGDTVQASTFTYPGTTSLGGVGAMTGGTVYFDSGVTHSLASAVASGQITA
                     TLPASRAVGADMIFEIDVVTADGRSLAGTLTVPLDDVLPVYAPS"
     gene            2204..4603
                     /locus_tag="PLQ54_02645"
     CDS             2204..4603
                     /locus_tag="PLQ54_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOS92186.1"
                     /translation="MSQGLSVGRACTYVLCEAHASHALHQLSETHDVRELSASGALNL
                     YGTADLRCWILGVAHGDATPFLSWAPGTCLVLDAATAVTGAELIEAYPAFLGALTRWA
                     RDVSATVVVQHDADEDDPRAVNEALQHLGAVVSGDWVVDNPFCAPAIDDGGDANRQLL
                     ADRLHVLAGLASPPEARELPELLEDAPDIIYRVDGRGRLTHLNAAFERATGRPRQQLI
                     GRPFSDLLAPEDARRGRQFIQRLLKGETVEEDLDFAVAGQPARPYSVLGHAVPGAGRS
                     MAAIGVARDVSLHKEMERQLLESETGYRAILDSAPVSVMLIDESGVVRDVNAWHVERI
                     GGPRASRADYIGQNLRTSPREAAAGLSSFYDRLCQGEPFDLSEVYLPETATGRPVWVN
                     IRGAPLYGDDGNMRGAIVIHEDITELKQMQQHMLVFERFRALGEMSAGVAHEFNNLLA
                     IILGQAELALMRLDRAEDVDARLRNIIRTATRGAETVRGLQAYARQRTKRPGATTDVN
                     SVVARAVEIACELARRLGETRERPVQIFTDLGDPPAVAADAAEFGDAVGAVIENAVEA
                     AAQGWGTVRVTTRSEDAHAVVEVSDDGPGIAATDMQRIFEPFFTTKGPQRNGLGLSVA
                     YGVVHRLGGGIVAEASDDGGALISIRIPSAHPQIGPVELEAAPPPRPSAHKPRVLVVE
                     DEESVLEVLADAVSTMGYEVNAVRDGQAAIELAGRHAFDVVITDLGMGQVSGLDVARV
                     FKSTHQPPVVVLLTGWGVELGDPEISQSGVDRVMAKPLKMDELKACLAECVQSRQGEA
                     R"
     gene            complement(4620..>5338)
                     /locus_tag="PLQ54_02650"
     CDS             complement(4620..>5338)
                     /locus_tag="PLQ54_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing C40 family peptidase"
                     /protein_id="HOS92187.1"
                     /translation="TGFRPVDTLSQPAASSAPRDPTTITATQVRVAATALSGDGLVQE
                     GLVNVRAGPGMGFNLIGQATRGTPLRVIGATNGWFLVRFASGAEGWVAGWLVATDQSI
                     AQQISPAQTPAGQPTSLGNSIAQYALGFVGTPYVFGGSSPSGFDCSGLVQYVLKHHNI
                     RVPRTSFEQWDVGTPVDPSRLIPGDLVFFANTYTAGVSHVGIYVGNGNFVHASRSGVG
                     VIVQPLKERAGSYCGARRVY"
BASE COUNT          835 a         1821 c         1812 g          870 t
ORIGIN      
        1 tccgcaactg ggcgaggagt tggtcgcgtt cggacgggcc gctcgcggtc gtgcgcgatc
       61 ccgccgatgc acccgccgga gccgtcttgc ccgaaccttt cgccgcctga gagagcgcct
      121 ccggcgactt ggcttcctcc gccggctggc tcgaagcgtc cgccgcaccc ttggtcgact
      181 cgccggcgcc ggtcgtgccc tgagtcgctc ctgcctcgtc ggcgctggtc ttctcgccgg
      241 tttccgcctc gggcggcgct gatggcggct ctgcttccag atcgaccgga ggccgagtgc
      301 tctccatcac cggagccgac ggcgtggacg gctcgtcgcc gctcgcgagg cggtcggaga
      361 tccgctggca tcccgccagc acggcgagac tgatcaccac gagaagcgcg acgataggga
      421 cccgctttcg gaccatccgc gatcactccc accggggcgg ccgcattata gcgaagtggc
      481 cggacgggcg gcaaccgagg tgcaccacac tgccacgacc gggggagaca ccaggctccg
      541 cccgaacgtc tatcttctgc ggacgtagcc agacctcatg cccggtcgcg tgtttgacgc
      601 cgcgcagcgg gggcctcgat aatggttgcg gtacgcttcg gcgcgtccgg accattcccc
      661 tgcgtcccgc caaacgcggc cggagccgat tccgggaggc gcgcgtggta catccaacga
      721 gattcccagg ctcaccgtgt ctgacggcgt ggctcgctgc gctcgccgtc ttgctcgccg
      781 gctgtggcgg ttctccgatc gatgtcacgc ccggtgggaa cggcaactcc aacaacaatg
      841 gcggcactgc cgcgttcggc gagggagacg cgttctacat caccgttccc gcgcccagcg
      901 gcacgggcaa cgcctacctg cctggacccg gcctttcctc accgagcggc agcgctaacg
      961 atccgcctct gccccccggc caggacccga cctctggcga gggggtcgtc ggcgtgttcc
     1021 tggaagggct cgacggcaca ctcactgggt catcattcga cgtcgcccga agcgaggccg
     1081 acgtgaatgc gtgtggcggt gggtgtctcc tcgcctcccg ctccgcgacg tcggggaacg
     1141 ccgtggtccg cgtcatcctg gccaacgcca ccggtacggt ctgggacgtg accgcccttg
     1201 gcaccgtcag cggcgatacc gtgcaggcca gcacattcac gtaccccggg acgacgtctc
     1261 tgggcggtgt gggggcgatg acaggcggaa cagtgtactt tgactctggg gttacccact
     1321 cgcttgcctc tgcggttgca tcggggcaga tcactgcgac gttacccgcc agccgagccg
     1381 tcggggccga catgatcttc gagattgacg tcgtgaccgc cgatgggcgg tccctggccg
     1441 gtactctcac cgtgccgctt gacgacgtgt tgccagtcta cgcccccagc taggtgcgcc
     1501 ggcgtgtgtt ggaccagtac ggcgaccgcc ccttctccgg attcacagcc aggcagcagg
     1561 gcaaggtggc tttggcaccg gcagtcgctg gcaccgaaac cggggatcgg ctcgcgggct
     1621 cccggcacgc gcatcgcggc ttcatggagc gaacactcga ggcggtgggg cgacagcacg
     1681 aaccggatgg cgatgatgcg gcccacggcc ctgaggtagt cgatatgcca ccggatcgcc
     1741 cccgctgggc gaaggtggcg cagaacgcgc tgccgaacac cgcccagagc gctcccggag
     1801 tagacatagg ttccttcgtc gacctcccag gtgccgaggg cgccgaccga gagccgagtg
     1861 gccggaaccc ggaggatgag ctggtaggcg ccgggaacac tgtccggtac ctggttgcgc
     1921 agagcttcca ggccccatac ggtgctgggc tcggccatct cggtgcctcc cggtcatcga
     1981 ggaagcgcgg ggtctgggac ccctgcacca cccaccgttc gtaggaggag ggcttcgcgg
     2041 ccggtacggc cgcccgcgga gccccagctg aatgggtcga agctcgaggg accatactcc
     2101 acctggaggc aggtcagtcc gcgatatccg cgatccgcgt cccggtgagt gcgtgtgcca
     2161 tatcgccgat gcaggggctc gcagggcgcg ggcgacgtgc tgggtgagtc agggactctc
     2221 tgtaggtcgg gcgtgcacct acgtcctctg cgaggcccat gcttcgcatg cccttcacca
     2281 gctttcggag acccacgatg tacgcgagct cagtgcatcg ggcgccctga acctctacgg
     2341 caccgccgac cttcgttgct ggatcctggg tgtcgcacac ggcgacgcca cgccgtttct
     2401 ctcgtgggcg ccggggacct gcctggtcct ggacgcggcg actgccgtca cgggtgccga
     2461 gctgatcgag gcctatccgg cgttcttggg tgcccttacg cggtgggctc gcgacgtgtc
     2521 cgccacggtg gtcgtccagc atgacgcgga cgaagacgac ccccgtgctg tcaacgaagc
     2581 actgcagcac ttgggtgccg tcgtgtcggg tgattgggtt gtcgacaacc ccttctgcgc
     2641 tcccgcgatc gacgacggcg gagacgccaa tcggcagctc ctcgcagacc gattgcacgt
     2701 cctcgccggt ctcgcgagcc ccccggaggc gcgcgagttg ccggagctgc tcgaggatgc
     2761 cccggacatc atctaccggg tcgatgggcg cggacgtctc acgcacctga acgccgcgtt
     2821 cgagcgggca acgggccgcc cgcgccagca gctcatcggg cgcccgttct cagacctgct
     2881 cgcccccgag gacgcacgtc gcgggcgcca gttcatccaa cgcctgctca agggcgaaac
     2941 ggtcgaggag gatctggact tcgcagtcgc cggtcagccc gcgcggccct actcggtgct
     3001 cgggcatgcc gtgcccggtg ccggtcgcag catggccgcc atcggcgtgg cgcgcgacgt
     3061 ctccctccac aaggagatgg agcgccagct tctcgagtcc gaaacaggct accgggccat
     3121 tctggattcc gcgcccgtgt cggtgatgct catcgacgag tccggcgtcg tccgcgacgt
     3181 caatgcctgg catgtcgagc gtatcggcgg acccagggcc tctcgggccg actatatcgg
     3241 acagaacctg cgaacctcac ctcgagaggc ggcagccggc ctctcaagct tctacgaccg
     3301 gctgtgccag ggcgagccgt tcgacctctc cgaggtgtac ctgccggaga cggccaccgg
     3361 gcgccccgtg tgggtcaaca ttcgcggagc cccgctctat ggcgacgacg gcaacatgcg
     3421 cggcgccatc gtgatccacg aggacatcac cgaactcaag cagatgcagc agcacatgct
     3481 tgtgttcgag cgtttccggg cactgggcga gatgtcggcc ggcgtggcac acgagttcaa
     3541 caacctgctg gccatcattc tgggccaggc tgaactggct ctgatgcggc tcgatcgagc
     3601 cgaggatgtg gacgcgcgtc tgcgaaacat catcaggacc gctactcgag gcgccgagac
     3661 ggtccgagga ttgcaggcgt atgcgcggca gcgaaccaag cggccgggag cgacgaccga
     3721 tgtcaacagc gtcgtcgctc gggccgtcga gatcgcctgc gagctggccc ggcgcctcgg
     3781 agagacccgc gagcggccgg tgcagatctt cacggacctg ggtgacccgc cggccgtcgc
     3841 cgccgacgcg gccgagtttg gcgatgcggt gggcgccgtc atcgagaacg ctgtcgaggc
     3901 tgcggcgcag ggctggggca ccgtccgtgt caccactcgc agcgaggacg cccacgccgt
     3961 ggtcgaggtt tctgatgatg gcccgggcat cgcggccacc gacatgcagc ggatcttcga
     4021 gcccttcttc acgacgaaag gtccccagcg caacggcttg ggcctgagtg ttgcctacgg
     4081 tgtcgtccac cgcctcggcg gcgggatcgt cgccgaggcc agtgatgacg gcggggctct
     4141 gatctcgatc cgaatcccct ctgctcatcc ccaaatcggg cccgtcgagc tcgaggcggc
     4201 tccgccgccg cggccgagcg cacacaagcc ccgggtcctc gtggtcgagg acgaggaatc
     4261 ggtactcgag gtcctggctg atgcggtctc gacgatgggc tacgaggtga acgcggtgcg
     4321 cgacggccaa gcggccatcg aactcgccgg gcgccacgcc ttcgacgtgg taatcaccga
     4381 tttgggcatg gggcaggtct cgggcctcga cgtggcgcga gtcttcaagt cgacgcacca
     4441 gccccccgtg gtcgtcctgc tgaccggatg gggagtcgag ctcggcgatc ctgagatctc
     4501 acaaagcggg gtcgaccgtg tgatggccaa acccctgaag atggacgagc tgaaggcttg
     4561 cctggctgag tgcgtccaga gccgacaggg cgaggcacgc tagcgtcaac gggccgccgc
     4621 tagtagaccc ggcgggctcc gcagtagctt ccggcgcgtt ctttgagcgg ctgcacgatg
     4681 acaccgactc cgctgcggga ggcatggacg aagttcccgt tgcccacgta gatccccaca
     4741 tgggagacgc ccgccgtgta ggtgttcgcg aagaacacca ggtcgccggg aatgaggcgg
     4801 ctcggatcga ctggggtgcc cacgtcccac tgctcgaagc tcgtgcgcgg cacacggatg
     4861 ttatggtgtt tcagcacata ctgcaccagg cccgaacagt caaatccgct gggtgagctg
     4921 cctccgaaaa cgtagggcgt gcccacgaat cccagggcat actgcgcgat cgagttgccc
     4981 agactggtgg gctgacctgc cggcgtctgc gccggtgaga tctgttgggc gatactctga
     5041 tcggtcgcca cgagccatcc ggcgacccag ccctcggctc ccgacgcgaa ccggaccagg
     5101 aaccatccat tcgtcgcgcc gatgacgcgc aagggcgtcc cgcgggtcgc ttggccgatc
     5161 aggttgaacc ccatgcccgg accggcacgg acattgacca agccctcctg aaccaacccg
     5221 tcgcccgaga gggccgtggc agccacgcgc acctgcgtcg ccgtgatcgt ggtcgggtcg
     5281 cgaggcgctg atgacgctgc gggctgggag agcgtgtcca cgggccggaa gcccgtcg
//
LOCUS       DAOSPL010000088         5314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_15595_length_5314_cov_3.034037, whole genome shotgun sequence.
ACCESSION   DAOSPL010000088 DAOSPL010000000
VERSION     DAOSPL010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15595_length_5314_cov_3.034037"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PLQ54_02655"
     CDS             <1..559
                     /locus_tag="PLQ54_02655"
                     /inference="COORDINATES: protein motif:HMM:NF025099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HOS92188.1"
                     /translation="AGRVLNGGLGFVPTALGTVFGILMLEGFLVTTLDVAVRLNRYLL
                     EELWGVLFAKPPGFLRQYWFNAGLSVALMLVTAASGAVEQIWPVFASANQLLAALGLL
                     VVSLWLLNRQTVPWFTMVPAMFMLLTTLGALGILVPKYLSGGKYVLLAAAILLFALAL
                     GVVVVASQGWLRWRLARRPADSPAS"
     gene            650..1723
                     /locus_tag="PLQ54_02660"
     CDS             650..1723
                     /locus_tag="PLQ54_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HOS92189.1"
                     /translation="MPPRAGKSPGQPFGDRFERIIHGFGSSPTGTRAVRFRWWWVAAP
                     FLLLFMARAYVVVPYGAGGVVFDRVRGVLPTALSEGFHSRIPLLQQVQIYDTKVCTYS
                     MTRKSKEGAAKGQDHLQALTSDGQKVDVEITVRYRLSRDTLPKLHQTVGPDYAERVIR
                     PVSRAVVRMVCAQHPVSDFTSQVRDVIQNDIRSQLADKFAPWYVTLEDVIIDEVEFSE
                     EFRAAVEEKQRALQEAEQMDYRIQEAAGEADRLRVEAEGDAERIRERGIALQRNPLLM
                     RFEYVKKISPNVKGLIVTKPELDEIKSAPRGFSPPAPRPLPDLTLRDLGLDHDLAVLR
                     AAGEEPDVKPDAPARVEREGGSR"
     gene            1720..2613
                     /locus_tag="PLQ54_02665"
     CDS             1720..2613
                     /locus_tag="PLQ54_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HOS92190.1"
                     /translation="MTATITGRRPALPRLAVGAAVLVGLIALAASVCVVPAGYPGVVF
                     SDFRGGVRPYTLREGMSLLVPFVEHPMLYEVRSRTYVFSSSELGEAQLRAGEVSAKTS
                     DAQAVYVDLTVRYHPNPKQVHRLHQQVGTEYEEKIVKPNTNGTARSVIAGYTAEEVFA
                     SKRQEIEDALRTRLSQVFAQYYIVLDDALIRNVRFSPEYHQAIEQKQIAQQRAQRKRY
                     ELQREQKDKLRKIIEARGQATAIEIRGQALAEHPSVVEYQYIEDLPSGTPVYVISGNS
                     IVNMSDLFKPQSEPVKPDKAR"
     gene            2909..>5314
                     /locus_tag="PLQ54_02670"
     CDS             2909..>5314
                     /locus_tag="PLQ54_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOS92191.1"
                     /translation="MRIRHTMLCTALMVAGACAQDYVWLEGESPTRTNYEVSGSGWGN
                     KQFLSGEAWLHISIDAERVAEQCPQGGIVLAYEFTLPSAGRYEVWNRIGFEFVRSPFE
                     WRIDGRAWATVAPDELTTDLMEIAEWCEVAWLQLGAAELTQGNHTLEIRLTPGTRTDN
                     GEEKPDRILYASDLLCIHKGPFRPNGRFRPDEDWQTDRDREAAARVVSVVAGRQGNRS
                     QTDLSGLWQVCRYDENEVTDRNGPTTTLPDQASAFWRAITVPGNKFETNPELRLCHRL
                     VYRTRVDIPQALAGQSLFLRLPSLSMIGSVLVNGQLCGWTKAMFALFECDITPAVKFG
                     GVNEIAVVIKDGYYAFSERKTGRSCRMSFNTPVTWMGSRSYVNQGFDYPIGAEEYAQN
                     AGILEAPSLVVSGAVYASDVFVKPSVTRGELGVQVTLRNPSASARTVQVACTATPVGA
                     GDSKAWAPETVVVPAGAEATIDIAEPWADPRLWWPDEPNLYELATRVSSDGTVVDTHT
                     TRFGFREWGWSGRSFTVNGVPWQFFADTTTDRDPEAAIRTWRANGQNMWRFWGRSFAG
                     LTKQRALDLMDERGIVVRRSGIFDGQGANYLAGLSDNPALYENWVAQMQAWVREERNH
                     PSILIWSMENEITFINSRNLGQADAVEPEIAKAADALMALDPTRPVMVDGGNCLKSNA
                     LPVNGVHYMESAWRSYPDEAYTLEQAYQAHVTNPGWNIWRLVPDKPIFMGESFYVRGS
                     TPSAFAQFGGEGCFSGWGSDTRIGVGRLCKMLAEGYRWHGVAATHFWMTSGETDLHYN
                     SWRP"
BASE COUNT          905 a         1643 c         1869 g          897 t
ORIGIN      
        1 tgcgggccga gtgctgaacg gggggctggg attcgtgccg acggcactgg ggacagtgtt
       61 cggcatcttg atgctggagg gattcctggt cacgacgctc gatgtggccg tccggctgaa
      121 ccggtacctg ctcgaggagc tgtggggcgt gctgttcgcg aagccacccg ggtttctccg
      181 gcagtactgg ttcaacgccg ggctttcggt ggcgctcatg ctcgtcacgg cggccagtgg
      241 ggcggtcgaa cagatctggc cggtgttcgc atcggccaac cagctgctcg cggcgcttgg
      301 cctgctcgtc gtgtcgctgt ggctgctgaa ccgtcagaca gttccatggt tcaccatggt
      361 tcctgccatg ttcatgctgc tcaccacgct gggcgcgctc ggcatcctgg tgccgaagta
      421 cctgtcggga ggcaaatacg tgctgttggc ggccgccata ctgctcttcg ccctggcgct
      481 gggcgtggtg gtggtggcga gccaggggtg gctgcgctgg cgcctggcga gacggcctgc
      541 ggattcgcct gcctcataga gtgaggctgc cgcgaccagg ctgtggctcg accgtccctg
      601 aacatgtggc tggttgcgtg gtcgaaggac atcaaggagg tcggtgtgta tgcccccacg
      661 tgccgggaag agccccggtc agccgtttgg ggatcggttt gagcggatca tccacggctt
      721 cggaagtagc ccaacgggaa cccgtgccgt ccggttccgc tggtggtggg tggccgcccc
      781 cttcctgctc ctgttcatgg cccgggccta tgtggtggtg ccctatggcg cgggcggtgt
      841 cgtgttcgac agggtccggg gtgtgctgcc gacggcgctg tccgagggct tccactcacg
      901 tattccgctg ctgcagcagg tgcagatcta cgacaccaag gtctgcacat actcgatgac
      961 gcgtaagtcc aaagaggggg ccgccaaagg gcaagatcac cttcaggcgc tgacatcgga
     1021 cggtcagaag gtcgatgtag agatcaccgt gcgctaccgg ctgtcgcggg acacgctccc
     1081 gaagctccac caaacggtgg ggcccgacta tgccgagcgg gtgatccgac cggtgtcgcg
     1141 ggccgtggtg cgcatggtgt gcgcgcagca tccagttagc gacttcacca gccaggtccg
     1201 cgacgtgatt cagaatgata ttcgctcgca actcgccgac aagttcgctc cgtggtacgt
     1261 gaccctcgaa gacgtgatca tcgacgaggt cgagttctcc gaggagttcc gcgccgcggt
     1321 cgaggagaag cagcgggcac tgcaggaagc cgagcagatg gactaccgga tccaggaggc
     1381 ggctggcgag gccgaccggc tccgtgtcga ggccgaaggc gatgccgagc ggatccggga
     1441 gcgcggtatc gccctgcagc gcaacccctt gctgatgcgg ttcgagtacg tgaagaagat
     1501 ctcgcctaac gtgaagggcc tgatcgtcac gaaaccggag ctcgacgaga tcaaatcggc
     1561 accacgcggc ttctcgcctc cggcgccgcg tccattgccg gatctcactc ttcgggacct
     1621 gggcctcgat cacgatctgg ccgtgctccg ggccgcaggt gaggagccgg acgtgaagcc
     1681 cgatgccccg gctcgtgttg agcgggaggg gggatcccga tgaccgcaac gatcaccggt
     1741 cgccggccgg cacttccccg gttggctgtg ggtgcagccg tgctggtcgg cctcatcgcg
     1801 ctggcggcct cggtgtgcgt ggttcccgca ggctatcctg gagtggtgtt cagcgacttc
     1861 cgaggagggg tgcgccccta cacgttgcgc gagggcatga gcctgctggt gcccttcgtc
     1921 gagcatccga tgctctatga ggtgcgcagc cggacctatg tcttctcgag cagcgagttg
     1981 ggcgaagcac aacttcgcgc cggcgaggtc agcgcgaaga cctcggacgc gcaggcagtc
     2041 tacgtcgacc tgacggtgcg ctatcacccc aacccgaagc aggtgcaccg gctgcatcag
     2101 caagtgggaa ccgagtacga ggagaagatc gtcaagccga acacgaacgg cacggctcgg
     2161 tccgtcatcg ccggctacac ggccgaggaa gtcttcgcca gcaagcgcca ggagatcgag
     2221 gacgcgctca ggacgcgcct gtcgcaggtg ttcgcgcagt actacatcgt gctggacgat
     2281 gcactgatcc gcaacgtgcg gttctcaccc gagtatcacc aagccattga gcagaagcag
     2341 atcgcgcaac agcgtgccca gcgcaagcga tatgagctgc agcgtgagca gaaggataag
     2401 ctccgcaaga tcatcgaggc ccgcgggcaa gcgacggcca tcgagatccg cgggcaggct
     2461 ctggcagagc acccatcggt ggtcgagtac cagtacatcg aggacctgcc gtcgggaacg
     2521 ccggtgtacg tcatcagcgg gaacagcatc gtgaacatgt cggacctgtt caagccgcaa
     2581 tcggaaccgg tgaagccgga caaggcgcgg tgaggccggc aggcagatgg ccgatggcag
     2641 taggcagccc ggggctccga tcccgggctg ttcgcgttcg gggctgcagg gctggaggcg
     2701 gggacctgcc gcggccgacc gcagccgggt ctccgacggg gcgagtgggt agcggaggag
     2761 cgtgacgtcc cgctcgcggg gcgcggcccg gcaccccgga cagctcacaa gccggcgccg
     2821 ccaggaatgc cccccctgtg ggcaggggca cctccgcgta gcgccgaagg ccctcccggc
     2881 gttctcttca tcgggagggc gatccgccat gcgaatccgc cacacgatgc tctgcaccgc
     2941 gctgatggtg gccggcgcct gcgcgcagga ctatgtctgg ctcgagggcg agagtccgac
     3001 tcggactaac tacgaggtga gtggctccgg ctggggcaac aagcagttcc tctcgggcga
     3061 agcctggctt cacatcagca tcgatgccga gagagtcgcg gagcagtgcc cgcagggcgg
     3121 catcgtgctg gcgtacgagt tcaccctccc ctcggcggga cgctatgagg tgtggaaccg
     3181 cattgggttt gagttcgtcc ggtcgccgtt cgagtggcga atcgatggcc gggcttgggc
     3241 cacggtggcg cctgatgagc tgaccaccga cctgatggag atcgccgagt ggtgcgaggt
     3301 agcgtggctg cagctgggcg cggcggaact cacacagggg aaccatacgc tcgagatccg
     3361 cctgacgccc ggcacccgca cggacaacgg cgaggagaag ccggacagga tcctgtacgc
     3421 ctccgacctg ctgtgtatcc ataagggtcc gttccgaccg aatggcaggt tccggcctga
     3481 tgaggactgg cagaccgacc gcgaccgcga ggcggccgcg cgagtcgtgt ccgtggtggc
     3541 ggggcggcaa ggcaaccgga gccagaccga cctgtcgggc ctttggcagg tctgccggta
     3601 cgacgagaac gaggtgacgg accgcaacgg cccgaccacg acgcttcccg accaggcctc
     3661 agccttctgg cgcgccatca cggttccagg caacaagttc gagacgaacc ccgagctccg
     3721 gctgtgtcac cgcctggtct accggacccg ggtggacatc ccccaggcgc tggcgggcca
     3781 gtcgctgttc ctgaggctcc cctcgctgag catgatcggt agcgtactgg tgaacggcca
     3841 gctgtgcggc tggacgaagg cgatgttcgc gttgttcgag tgcgacatca cgccggctgt
     3901 gaagttcggc ggcgtgaacg agatcgccgt ggttatcaag gatggctact acgccttcag
     3961 cgagcggaag acaggccgca gctgccggat gagcttcaac acgcccgtga cctggatggg
     4021 ctcccgcagc tacgtgaacc agggcttcga ctaccccatc ggcgccgagg agtacgcgca
     4081 gaatgcgggc attctggagg cgccgagcct ggtggtcagc ggcgcggtgt acgcctcgga
     4141 cgtgttcgtg aagccgtcgg tcacgcgcgg agagctcggc gtccaggtca ccctccggaa
     4201 cccttcggca tcggctcgga cggttcaggt cgcctgcacc gcgacaccgg tgggcgccgg
     4261 ggactcgaag gcgtgggctc ccgagaccgt tgtggtaccg gctggggcgg aggccacgat
     4321 cgacatcgcg gaaccgtggg cggaccccag actgtggtgg ccggacgagc cgaacctcta
     4381 cgagctcgcc acgcgcgttt cctccgacgg caccgttgtc gatacccaca ccacccggtt
     4441 cggtttccgc gagtggggtt ggtcggggcg ctcgttcacg gtcaatggtg tgccgtggca
     4501 gttcttcgcc gatacgacca ccgaccggga ccccgaggct gcgatccgca cctggcgcgc
     4561 caacgggcag aacatgtggc gattctgggg gcggagcttc gccgggttga ccaaacagcg
     4621 agccctggac ttgatggacg agcggggcat cgtcgttcgc cgcagcggca tcttcgacgg
     4681 gcagggcgcg aactacctgg ccggcctgag cgacaacccg gcgctctatg agaactgggt
     4741 ggcgcagatg caggcgtggg tgcgcgagga gcgcaaccat cccagcatcc tgatctggtc
     4801 aatggagaat gagatcacgt tcatcaactc acgcaatctg ggtcaggccg atgcggtgga
     4861 gccggagatc gccaaggcgg ctgacgcgct gatggccctc gaccccaccc gacccgtaat
     4921 ggtcgatggg ggtaactgcc tcaagtccaa cgccctgccg gtaaatggtg tgcactacat
     4981 ggagagcgcg tggcgcagct atcccgatga ggcgtacacg ctcgagcaag cgtaccaggc
     5041 ccatgtgacc aaccccgggt ggaatatctg gcggcttgtg cccgacaagc ccatcttcat
     5101 gggcgagtcg ttctacgtcc gtgggtcgac gcccagcgcg ttcgcccagt ttggcggcga
     5161 ggggtgcttc agcggctggg ggagcgacac gcgcatcggt gtggggcgac tgtgcaagat
     5221 gctggccgag ggttaccgct ggcatggcgt ggccgcaacg cacttctgga tgacgtcggg
     5281 cgagaccgat cttcactaca actcgtggcg gccg
//
LOCUS       DAOSPL010000089         5307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_15620_length_5307_cov_4.706398, whole genome shotgun sequence.
ACCESSION   DAOSPL010000089 DAOSPL010000000
VERSION     DAOSPL010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15620_length_5307_cov_4.706398"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..1599)
                     /locus_tag="PLQ54_02675"
     CDS             complement(250..1599)
                     /locus_tag="PLQ54_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS92192.1"
                     /translation="MDKLDGLSRRGFLKSVATAAVAAHVGMTAVRAQDEAPAAPEAPP
                     FRFGLIGCGGQGRVALMGSAKRIPGVQFVGVCDIRQENLDRAKEQAGEGAQTFTDYRE
                     MINNLEMDAVIVATPLTLHRDITVAALEKGLPVFCEKCMAYTVDQCKDMLRAQQAAGK
                     TLQIGHHLRYHPLYHHAKKAFIGTGLLGDITAVYGQWNRNADWRRQVSEADAAGDYTA
                     WGFDTPERLVNWRLYKDLSGGLTTELAGHQIDVVNWMLDATPSAVTGVGGIDHWKDGR
                     TVPDNIHLTFEYPNGVKFDYQALTTNAFSMFGSECFEMIKGTKGTLVLTHLWGGPLKS
                     KGWFFLEKGVQEELWMDAAHKEKVGENQAIILDCTVTEGTRIAGQPYDKLMDNQGNLK
                     KPTYQLEFEEFIQSVRENKIPTCDGNVGIRSAAAALLANQAMEEQRRIEFSPDLYEL"
     gene            complement(1667..2710)
                     /locus_tag="PLQ54_02680"
     CDS             complement(1667..2710)
                     /locus_tag="PLQ54_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOS92193.1"
                     /translation="MLPDAVETEFTSQAEGAGETRPFRVRLRRPAMATQFELILDGDR
                     DPKTLGSMAEEAYALVQQLEQQLSAYLPASELCWINESAHAGPVPVEPGLFRLLLQAR
                     NIWEETSGAFDITVGPLVKSWGFFRRQGRMPAEGDLARAMESTGMDHVALDEQKRTVR
                     FLCPGVELNLGAIGKGFAIDEVMARLRSWGVTCALAQLGGSTVAVMGEPPVSQGWLLG
                     VRPGGQRSPRVGAVRLHNQAISTSGDFEQYFEVDGRRFGHIIDPRTGRPAEGRLSACS
                     ISASATRSDALSTAFVVMGSERAEAFCRSRGEAAVLMEPGADGALAARAIACTLLPAS
                     DDTADRSGQNAPG"
     gene            complement(2751..4082)
                     /locus_tag="PLQ54_02685"
     CDS             complement(2751..4082)
                     /locus_tag="PLQ54_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOS92194.1"
                     /translation="MEITVYNQNFGLVKDVRSIDLPEGRSVIQFMDVAAQIDPTSVHF
                     KSLTDPTGVEILEQNYQFDLVDRAKLMQKYLGKEIRITRYDQDGQPVETQEGVLLGAE
                     GGRPGVVRIGDEIVLDPQGTVILPKLPEGLIVRPTLVWDLATAKAGKHQVELSYMTTG
                     LTWAADYVAVVNEDDTKADLNGWVTLTNMSGARYPDATLKLIAGDVRRVAPMPPPAPE
                     APMAMPMEAGMGGGGFQEKAFFEYHMYTLQRPATIAENETKQVTLLSAPGATIKKVYV
                     LDPAVAPVAPSLGGPGDKRKVQVKIEVANKEESGLGMPLPKGKVRVYKADTDGSLQFV
                     GEDLIDHTPKDETVRLHIGDAFDLVGERKVMDHRQLGDRAQQEKIQFSLRNHKEKDTV
                     EITAVEHLWGEWTIENETTPFVKKDAQTAEFTVTVEPGQEKTIEFTVTTRW"
     gene            complement(4208..>5307)
                     /locus_tag="PLQ54_02690"
     CDS             complement(4208..>5307)
                     /locus_tag="PLQ54_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92195.1"
                     /translation="EIRITRYDQDGQPVETQEGVLLGAEGGRPGVVRIGDEIVLDPQG
                     TVILPKLPEGLIVRPTLVWDLATQKAGKHQVELSYMTGGLTWAADYVAVVNQDDTKAD
                     LNGWVTLTNMSGARYPDATLKLIAGDVRRVQPEEPEMMYAGAAVMREAVADKGFQEKA
                     FFEYHMYTLQRPATIAENETKQVTLLGAPGVAIKKVYVFDPATAPVVYSPGGDTNKRK
                     VQVKIEVANNEASGLGMPLPKGKVRVYKADTDGSLQFVGEDLIDHTPKDETVRLHIGD
                     AFDLIGERKVMDHRQLGDRAQQEKIEFSLRNHKEKDAVEITAVEHLWGDWTIEGETTP
                     FVKKDARTAEFTVTVEPGQEKTISFTVTTRW"
BASE COUNT          820 a         1807 c         1678 g         1002 t
ORIGIN      
        1 ccccgcgctt ccgcgccccc caggatacgc gacacttcca gcgaccgagt gggtacacct
       61 gccccgcgaa ggccgcttgg ggagccgggc agtcaccccg gagctccgcg ccgactcgcg
      121 cagacgcact actcgacggc ggagcgacga tggcgcacgg gagagcacag gcaaaacgaa
      181 ggtccggcca gctgggtcag ccacggcgac tgcgccgcgg cgggctggcc ggacctcttc
      241 gccacctcgc tagagctcgt acaggtcggg gctgaactcg atccggcgct gctcctccat
      301 ggcctgattg gcgagaaggg ccgcggcggc gctgcgaata cccacattgc cgtcacaggt
      361 gggaatcttg ttctcccgta cggactggat gaactcctcg aactcgagct ggtacgtcgg
      421 cttcttcagg ttaccctggt tgtccatgag cttgtcatag ggctgaccgg caatccgggt
      481 gccctccgtg accgtgcagt cgaggatgat cgcctggttc tcgcccactt tctccttgtg
      541 ggccgcatcc atccatagct cctcctgcac gcccttctcc aggaagaacc aacccttgga
      601 cttcagcggg ccgccccaaa gatgggtgag gaccagggtg cccttggtgc ccttgatcat
      661 ctcgaaacac tcggagccga acatgctgaa tgcgttcgtc gtcagcgcct gatagtcgaa
      721 cttgacgcca ttggggtact cgaatgtgag gtggatgttg tcgggcacgg tgcgcccgtc
      781 tttccagtgg tcgatgcccc ccactccggt gacagccgac ggcgtcgcat caagcatcca
      841 gttgaccacg tcaatctggt ggcctgccag ctcggtggtg agaccgcccg agaggtcctt
      901 gtacagccgc cagttcacca ggcgctcggg cgtatcgaag ccccaggccg tgtagtcccc
      961 ggccgcgtcg gcctcagaga cctgccggcg ccagtcggca ttccgattcc actggccgta
     1021 gaccgccgtg atgtcgccca gaagcccggt gcctatgaag gccttcttgg catggtgata
     1081 cagcgggtga tagcgcagat ggtggccgat ctggagtgtc ttgcctgccg cctgctgggc
     1141 ccgaagcatg tccttacact gatcgacggt gtaggccatg cacttctcac agaaaacggg
     1201 caggcctttc tcgagcgcgg cgacggtgat gtcgcgatgg agcgtgagcg gtgtcgcaac
     1261 gatgaccgcg tccatctcga ggttgttgat catctctcgg tagtcggtga acgtctgggc
     1321 gccctcgccg gcctgttcct tggcgcgatc caggttctcc tgacggatgt cgcacacgcc
     1381 gacgaactgc acgccgggta tgcgcttggc ggagcccata agcgccacgc ggccctggcc
     1441 accgcaacca atgaggccga agcggaaagg cggcgcctcc ggagcggccg gggcctcgtc
     1501 ctgggcgcgc accgcggtca tgcccacatg cgccgccacc gccgcggtcg ccaccgactt
     1561 gaggaaccct ctccggctga ggccgtccag tttgtccatt gtgtccatcc tcccccgtca
     1621 tgcgggcgtc cgctccagtc gtccgcgagc ggcatgccct ggccccctat ccgggggcgt
     1681 tctggcccga tcggtccgcg gtatcgtccg atgcgggcaa cagtgtacac gcaatcgcgc
     1741 gagccgccaa agcaccgtcg gcaccgggct ccatgagcac ggccgcctcg ccgcgggacc
     1801 ggcagaaggc ctcggccctc tccgagccca tcaccacgaa agccgtcgat agcgcatccg
     1861 accgggtggc cgaggctgag atactgcagg cgctcaggcg cccctcggcc ggacgaccgg
     1921 tccgcggatc gatgatgtgt ccgaagcgcc gcccgtcgac ctcgaagtac tgctcgaagt
     1981 ctccggaggt cgagatggct tggttgtgaa gccgcaccgc acccactcgc gggctcctct
     2041 ggcctccagg ccggacgccc agcagccacc cctgggacac gggcggctcg cccatgaccg
     2101 ccaccgtgct gccgcccagt tgtgccaggg cacacgtgac cccccaactc cgtaggcgag
     2161 ccatgacctc gtcgatggcg aagcctttgc caatggcccc aaggttcagc tcaacgcctg
     2221 ggcacaagaa ccggacggtc cgcttctgtt cgtccagcgc cacgtggtcc atcccggtgg
     2281 actccatcgc gcgtgcgagg tcgccctcgg cgggcatccg tccctgccgg cggaagaaac
     2341 cccagctctt caccagtggg ccgaccgtga tgtcgaacgc ccccgatgtc tcctcccaga
     2401 tgtttcgggc ctgcaggagc agccgaaaga gaccgggctc caccggcacc ggaccagcgt
     2461 gggcgctctc gttgatccaa cacagctcac tggcagggag gtaggcgctg agctgctgct
     2521 cgagctgctg gaccagcgcg taggcttctt cggccatgct tccaagtgtc ttcggatcgc
     2581 ggtcgccatc gaggatgagc tcgaactgcg tcgccatagc cgggcggcgc aggcgaacgc
     2641 ggaagggccg ggtctccccg gccccctcgg cctgtgacgt gaactcggtc tccacggcat
     2701 cgggcagcat gacgcacttt agacgcgtcg cacgccctgt aggttcggcc ctaccaccgc
     2761 gtggtcacgg tgaactcgat ggtcttctcc tggccgggct cgaccgtcac cgtgaactcc
     2821 gctgtctggg catccttttt gacgaacggc gtggtctcgt tctcgatggt ccattcgccc
     2881 cataggtgct ccacggcggt gatctcgacc gtgtccttct ccttgtggtt gcgcagcgag
     2941 aactggatct tctcctgctg agctcgatca ccgagctggc ggtgatccat caccttgcgc
     3001 tcgccgacaa ggtcgaacgc gtcaccgatg tgcaggcgaa cggtctcgtc cttgggcgtg
     3061 tggtcgatca ggtcctcgcc gacgaactgc agtgagccat cggtgtcggc cttgtagacg
     3121 cggaccttgc ccttgggcag gggcatgccg aggccggact cctccttgtt ggcgacctcg
     3181 atcttgacct gaaccttgcg cttgtcgccc gggccaccga gggacggagc aacgggcgcc
     3241 accgccgggt ccagcacgta caccttcttg atcgtcgcgc cgggcgccga gagcagtgtg
     3301 acttgcttgg tctcgttctc ggcgatcgtg gcagggcgct gcagcgtata catgtggtac
     3361 tcgaagaagg ccttctcctg gaacccgccg ccgcccatgc cggcctccat cggcatggcc
     3421 atgggcgcct ctggggccgg gggaggcatc ggcgcgacgc ggcgcacgtc gccggcgatg
     3481 agcttgaggg tggcatcggg ataccgcgcg ccactcatgt tcgtcagcgt gacccagccg
     3541 ttgaggtccg ccttggtgtc gtcctcgttc accacggcga cgtagtccgc agcccaggtc
     3601 aggccggtcg tcatgtagga gagctcgacc tgatgcttcc cggccttggc cgtggccaga
     3661 tcccagacca gggtgggccg gacgatcagg ccctcgggca gcttcggcag tatgaccgtg
     3721 ccctgcgggt cgagtacgat ttcgtcgccg atgcggacca cgcccggccg gccaccctcg
     3781 gcgcccagca gaacgccctc ctgcgtctcc acgggctgac cgtcctggtc ataccgcgtg
     3841 atacggatct ccttgcccag gtacttctgc atcagcttgg cgcggtcgac gaggtcgaac
     3901 tgatagttct gctcgaggat ctccacaccc gtcggatcag tcagcgactt gaagtgcacc
     3961 gacgtcgggt cgatctgggc cgccacgtcc atgaactgga tgaccgaccg cccctcaggg
     4021 agatcgatgg accgcacatc cttcaccaga ccgaagttct ggttgtaaac agtgatctcc
     4081 attttctccg ttggtccgac ctgggcgacg gagagtgagg ccgatgctgc catggcaacc
     4141 gctagcgcca gtactttcat gtgctgcccc cttatcccgt tcatcggaca tgcgggccgg
     4201 attgcgccta ccaccgcgtc gtcacggtga aactgatggt cttctcctgg ccgggctcga
     4261 cggtgacggt gaactcggcg gtccgggcgt ccttcttcac gaacggcgtg gtttcgccct
     4321 cgatggtcca gtcgccccag aggtgctcca cagcggtgat ctcgaccgcg tccttttcct
     4381 tgtggttgcg tagcgagaac tcgatcttct cctgctgagc ccggtcgccg agctgacggt
     4441 gatccatcac cttgcgctcg ccgatgaggt cgaacgcgtc gccgatgtgc aggcggaccg
     4501 tttcgtcctt gggcgtgtgg tcgatcaggt cctcgccgac gaactggagg gagccgtcgg
     4561 tgtcggcctt gtagacgcgg accttgcctt tcggcagggg catccccagg cccgacgcct
     4621 cgttgttggc cacctcgatc ttgacctgga ccttgcgctt gttcgtgtcg ccgccagggg
     4681 agtagaccac cggcgccgtg gccgggtcga agacatagac cttcttgatg gccacaccgg
     4741 gagccccaag gagagtgacc tgcttggtct cattctcggc gatggtcgcg gggcgctgca
     4801 gcgtgtacat gtggtactcg aagaaggcct tctcctggaa gcccttgtcg gccactgcct
     4861 ccctcatcac ggccgcaccg gcgtacatca tctctggctc ttcgggctgc acgcggcgca
     4921 cgtcgcccgc gatgagcttc agagtggcat cagggtaccg ggcgccgctc atgttcgtca
     4981 gtgtgaccca gccgttgaga tcggccttcg tgtcgtcctg gttcaccacg gccacgtagt
     5041 cggccgccca ggtcaggcct ccggtcatgt aggacagctc gacctggtgc ttcccggcct
     5101 tctgcgtggc gagatcccac acgagggtgg ggcgcacgat caggccctcg ggcagcttcg
     5161 gcagtatgac cgtgccctgc gggtcgagta cgatttcgtc gccgatgcgg accacgcccg
     5221 gccggccacc ctcggcgccc agcagaacgc cctcctgcgt ctccacgggc tgaccgtcct
     5281 ggtcataccg cgtgatacgg atctcct
//
LOCUS       DAOSPL010000090         5307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_15624_length_5307_cov_2.957540, whole genome shotgun sequence.
ACCESSION   DAOSPL010000090 DAOSPL010000000
VERSION     DAOSPL010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15624_length_5307_cov_2.957540"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2094
                     /locus_tag="PLQ54_02695"
     CDS             <1..2094
                     /locus_tag="PLQ54_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOS92196.1"
                     /translation="AMSALGLATCVVGRGDRVPYRPEIWLQEQVVGDCREALEAGASA
                     RRLVPERDWQEWRARVLGVVREPFPAVLFARGRDLKARVVSRHEFDTYRIENVLFESL
                     PGWEVNGSVYLPREPGVYPGVVCPTGHSSKTGPSYQQPAQVFARNGYVAISFDPPGCA
                     GELGPGNDHFTHGFLGYLTGFWSQTHFVADAIACMDYLSTRPDVDAGLGFAVTGVSGG
                     GLTSIFTAMLDERVRFVAPVCCVSTHETLHLDGLYTSCPEQFGPGYISGMVDFADYLG
                     ALVPKPLLIVSGRGDEVFKHEAVLQVADDVEAIYEAADAKGDFGLFVDQESGHAYTVA
                     MANEVVRWMNRALKGIDADALPLTADEVPLTDPANLMCHPSTAVNMATINRAEAERLL
                     ALRQRAPRDGRLGRMRAAARRLLAVDPDARPHAVIRLSDPQTSWHALVGEIGIRPLRG
                     TDGYVPGVLFTHVEDRSPRPGLLWIDDQGKWAAFRHDGFLARPLRMFEASCSPEQPRI
                     LSIDVAGLGSLSPEPTAYDLAGWNDIERNLTYLSIANARPIMGLRVRDALCAFAELRD
                     RPDVDPDRLMVGGRGIGAVVALHVALLRPEVKRLICLEGLSDYGSYTRNGAFAWRESL
                     MIPGVLKHYDLPEVVAALRGCECILANLLDSMRSPVPQAEAAALYAEAIAGGATVVCG
                     ADGGAAVAEAVSAAW"
     gene            2178..5252
                     /locus_tag="PLQ54_02700"
     CDS             2178..5252
                     /locus_tag="PLQ54_02700"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOS92197.1"
                     /translation="MPDRSTCIVATAMVCVWAAACPGQPGVRELALAGGNLESEWRVS
                     TDLARTCGKGGWAEPDVDDSVWRVLGVPGLSGDHGTTHGGPEGTRHGPVSLLPAEYVE
                     RPVLPMKPSGAGLRERFANPPADRRILKIVHSLPADPAQHETFFTTLLEQGFGGVVCH
                     VAFDQYLQSDALWDSFVHGARLAHRLGMALWLYDEEGYPSGNAGGLVLKEHPEWQAKG
                     LHIAETTTNGKTVDLPLPPGQVRLAVACPVEAGRIDAEDPTDLSRSIRDGALRWAPPA
                     GEWHVFVFAENVLHEGTHAVSNAFRAQPYINIMDPRPVKRFIELTHEEYLRRLPELKD
                     LFVATFTDEPSLMSLFLKEQQWAAVPWADDFAETFERAKGYDLLPWLPALVADAGSGG
                     HRARCDFWDHVAQRVADSFFGQIQEWCHKVGLPSGGHLLWEESIASHCALYGDLYRCA
                     ERLDYPGIDCLTSSPPNVPWHIAKLLGSVAHLHGAPVVMSETSDFAQVYRPAGNTSPI
                     EPVGVSDIVGTCNRLYVAGVNTTNSDYSWAGLDTAQMRRISEFVGRCGTMLTGGRPIT
                     DVAVLYPIESVWAHMTPATLGATRSPKVHRIDRVWQECQNVLFRGQRDFEVVPSHAIA
                     GAGIEDGVLVAGPLRFRAVVLAATDTIPTEAWQRLSEFWDRGGIVLCAGDIPANSTQS
                     LRDTRVGALSARMLGLADRDDPGEGPAIVSHGNAAGGVAAFVPEALLPAVPRLLDRLL
                     ARDFSAGDASGALRYAHWSIDGREVYYVINDSASAVRARCTFAADGEAELWDPATGTV
                     TAAETSEADGGRSVSVSLEPYGATFVVFPRAVARARRETQGALEAVVSSTPLSQAVGA
                     EVRFEAAGAEHVTRSITMAPAVMGGDRPGTVLIDATIQQGRTDSHCIASARLPAGTDL
                     RGLDAVRVCTYVPEGQGACGAALLVLLHERGGAVYLAPAGRTLSEPGWQESLVWFEGF
                     SLAEWTKDDNGRLDLDQIGAISVGWGGYSGKSNERVRFALAPVELVQFDWEG"
BASE COUNT          774 a         1742 c         1915 g          876 t
ORIGIN      
        1 gccatgagtg cactcgggct ggccacgtgt gtcgtcggcc gcggtgaccg ggttccctat
       61 cggcccgaga tctggctgca ggagcaggtt gttggtgact gccgcgaggc cctcgaggca
      121 ggcgcgtcgg cccggcggct ggtgccggag cgcgactggc aggaatggcg cgcgcgcgtt
      181 cttggcgtcg tgcgggaacc cttccccgcc gtgctcttcg cgcgggggcg tgacctgaag
      241 gctcgcgtcg tatctcggca cgagttcgac acctaccgca tcgagaatgt gctgttcgag
      301 tcgctgcccg gttgggaagt taacgggagc gtctacctcc cgagggagcc gggggtctat
      361 ccaggcgtgg tgtgccccac cggccacagc agcaagacgg ggccgagcta ccagcagccg
      421 gctcaggtgt tcgctcgaaa cggctatgtg gccatctcct tcgatccgcc gggctgcgcg
      481 ggcgagctgg gccccgggaa cgaccacttc actcacgggt tcctgggcta cctcacgggc
      541 ttctggtcgc agacgcactt cgtggccgac gcgatcgcct gcatggacta cctgtcgacg
      601 cgccccgatg tggacgccgg cctcggtttc gcggtgacgg gcgtctcggg tggcgggctg
      661 accagcatct tcactgccat gctcgacgag cgtgtccggt tcgtggcccc ggtgtgctgc
      721 gtctccaccc acgagaccct ccacctggat gggctctaca cgagctgccc cgagcagttc
      781 gggccggggt acatctcggg catggttgac ttcgcggact acctcggggc cctggtgcca
      841 aagccgctcc tgattgtctc ggggcgtggt gacgaggtgt tcaagcacga ggctgtccta
      901 caggtggccg atgatgtcga ggccatctac gaggcggccg atgcgaaggg tgacttcggg
      961 ctgttcgtcg accaggaatc gggtcatgcc tacacggtgg ccatggccaa cgaggttgtc
     1021 cggtggatga accgggcgct gaagggcatc gatgccgacg cgctgccgct cacggctgac
     1081 gaggtgcctc tgaccgaccc cgccaacctg atgtgccacc cgagcaccgc cgtgaacatg
     1141 gccaccatca accgggccga agccgaacgc cttctggccc tgcggcagcg tgctccgcgc
     1201 gacggccgac tcgggcgcat gcgcgcagcg gcccggcggc tgctcgcggt ggacccggat
     1261 gcccggccgc atgccgtcat ccggctatcc gacccccaga ccagctggca tgcgctggtg
     1321 ggggagatcg gcattcggcc gctcaggggc acggacggat acgttcccgg cgtgctgttc
     1381 acgcatgtcg aggaccgatc gccgcggccg ggtctgctgt ggatcgacga ccaggggaag
     1441 tgggcggcct tccggcacga cgggttcctc gcaaggcccc tgcgcatgtt cgaggccagc
     1501 tgttcgccgg agcagccgcg cattctgagc atcgacgtcg ccgggctggg cagtctgagc
     1561 ccggagccca cggcgtacga cctggccggg tggaacgaca tcgagcgcaa cctcacctac
     1621 ctctccatcg ccaacgctcg gccgatcatg gggttgcgcg ttcgcgacgc gctgtgtgcg
     1681 ttcgctgaac tccgggaccg gccagacgtg gatcccgacc gtctgatggt cggcggccgg
     1741 ggcatcggag cggtcgtggc gctgcacgtg gcgctactga ggcccgaggt gaagcgcctg
     1801 atctgcctcg agggcctgtc ggactacggc tcgtacacga ggaacggcgc cttcgcctgg
     1861 cgtgagagcc tgatgatccc cggtgtgctg aagcactatg acctccccga ggtggtggcg
     1921 gccctgcgcg ggtgcgagtg catcctcgcc aacctgctgg acagcatgag gtcgcccgtg
     1981 ccgcaggccg aggccgccgc gctgtacgcc gaggctatcg cggggggcgc cacggtggtc
     2041 tgcggggccg acggcggagc cgccgtggcc gaggccgtct ccgcggcatg gtagcgcgtc
     2101 ggcgggaccc acggtcacgc gagtgaaacg cctgtcgccg gccgacgtcc cgattgggag
     2161 tggaaggggc gatctcgatg ccggaccgta gcacttgcat cgtcgccacg gccatggtgt
     2221 gcgtgtgggc tgcggcgtgc ccgggtcaac ccggagttcg agagctggcg cttgcgggcg
     2281 gcaacctcga aagcgagtgg cgggtctcca ccgacctcgc gcggacgtgt gggaagggcg
     2341 ggtgggcgga gccggacgtc gacgactcgg tgtggcgggt cctgggagtg ccgggcctct
     2401 cgggggatca tggaaccacg catggcggcc cggaaggtac ccgccatggc ccggtgtcac
     2461 ttctccccgc ggagtatgtc gagaggccgg tcctgcccat gaagccatcg ggcgcagggc
     2521 tccgcgagcg gttcgccaac ccgccggccg accggcgaat cctcaagatc gtgcacagcc
     2581 tgccggccga cccggcccag cacgagacgt tcttcacgac gctgctcgaa cagggcttcg
     2641 gcggcgtggt ctgccacgtc gccttcgacc agtatctgca gagcgacgcg ttgtgggact
     2701 ccttcgtcca cggcgcacgg ctggcccaca gactgggcat ggccctgtgg ctctacgacg
     2761 aggaaggata ccccagcggc aatgccggcg gcctggtgct caaggagcac cccgagtggc
     2821 aggccaaggg actgcacatt gccgagacga ccaccaacgg caagaccgtc gatctccccc
     2881 tgccacccgg ccaggtgcgg ctcgcggtgg cgtgtccggt cgaggcaggg cggatcgatg
     2941 ccgaagaccc cactgacctg tcgagatcga tccgcgacgg cgcgcttcgc tgggcaccgc
     3001 cagctggcga gtggcacgtg ttcgtgttcg ccgagaacgt cctgcacgag ggcacccacg
     3061 cggtttcgaa cgcgtttcga gcgcagccct atatcaacat catggatccg cgcccggtaa
     3121 agcgcttcat cgagctgacc cacgaggagt atctgaggcg cctgccggag ctcaaggacc
     3181 tgtttgtggc gaccttcacc gatgagccgt cgctgatgtc gttgttcctg aaggagcagc
     3241 agtgggcggc ggtgccgtgg gcggatgatt tcgcggagac ctttgagcgc gccaagggct
     3301 atgacctgtt gccgtggctg ccggccttgg tcgccgatgc cgggtccggt ggccaccggg
     3361 cgcggtgcga cttctgggac catgtcgccc agcgggtcgc cgactcgttc ttcgggcaga
     3421 ttcaggagtg gtgccacaag gtgggcctgc cctcgggcgg gcacctgctg tgggaggaga
     3481 gcattgccag ccactgcgcc ctctatggcg acctctaccg ctgcgccgag cggctggact
     3541 atccgggcat tgactgcctg acttcgtcgc cgccgaacgt gccatggcat atcgcgaagc
     3601 tgctcggctc ggtggcgcac ctgcacggcg ctcccgtcgt catgtcggag acatcggact
     3661 tcgcgcaggt ctaccggcct gcagggaaca ccagccccat cgagcccgtc ggagtttcgg
     3721 acatcgtggg gacctgcaac cgcctctatg tcgctggcgt gaacacgacg aactccgact
     3781 actcgtgggc cggtctcgat acagcgcaga tgcgccgcat cagcgagttc gtcgggcgct
     3841 gcggcaccat gctcaccggc ggccggccca tcactgatgt ggcggtgctg taccccatcg
     3901 agagcgtgtg ggctcacatg acacccgcga ctctcggcgc cacgcgttcg ccgaaggtgc
     3961 accgcatcga ccgcgtgtgg caggaatgcc agaacgtgct cttccgtggc cagcgggact
     4021 tcgaggtggt cccgtcacat gccattgcgg gagcgggaat cgaagacggc gtcctcgtgg
     4081 ccggtccgtt gcggttccgc gcggtggtgc tcgccgccac cgacacgatt ccgaccgagg
     4141 cctggcagcg actcagcgag ttctgggacc gaggcgggat cgtcctctgc gccggggaca
     4201 tcccggcgaa cagcacgcag agcctgcgtg atacgcgcgt tggggcgcta tcggcccgta
     4261 tgctcggcct cgcggaccgc gacgacccgg gtgaaggccc agcgatcgtg tcccatggca
     4321 acgcggcagg cggggtagcc gcattcgtac ccgaggcgct gctccccgcg gtgccgcggc
     4381 tgctcgatcg acttctcgcg cgcgacttct ccgcgggcga tgcttccggc gcgcttcgct
     4441 atgctcactg gagcatcgac gggcgcgagg tctactacgt gatcaacgac tcggcaagtg
     4501 ccgtaagggc ccgctgcaca ttcgcggccg acggtgaggc cgagttgtgg gatccggcga
     4561 cgggcacggt gacggctgcg gagacctctg aggccgatgg cggacggtcc gtgtcggtct
     4621 ccctggagcc ctatggggct accttcgtcg tcttcccccg agccgtggcg cgggcgaggc
     4681 gggaaacgca gggggcgctt gaggcggtgg tcagctcgac gccgctctcg caggccgtcg
     4741 gcgccgaagt gcggttcgag gcggcgggcg ccgagcacgt cacgcgctcg atcacaatgg
     4801 cgccggcagt catgggcggc gaccggccgg gcaccgtcct catcgatgcc acgatccagc
     4861 agggccgtac cgacagccac tgcatcgcca gcgcgcggct cccggccggg accgacctcc
     4921 ggggtctcga cgcggtgagg gtctgcacct acgtgcccga gggccagggc gcttgtggcg
     4981 ccgcgctgct ggtgctcctg cacgagaggg gcggggcggt ctatctggcg cccgcggggc
     5041 gcacgctatc ggagccgggc tggcaggagt cgcttgtctg gttcgagggc ttctcgctgg
     5101 ccgagtggac gaaggatgac aacggccgac tggacctcga ccagatcggg gccatcagtg
     5161 ttggctgggg ggggtactcc ggcaagagca acgagcgcgt gaggttcgcc ctggccccgg
     5221 tcgagcttgt gcagttcgac tgggaggggt agggggcatt ggcggtaact taggcctggg
     5281 cggtgcgcgg gaggcccgta cacggca
//
LOCUS       DAOSPL010000091         5305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_15632_length_5305_cov_3.296952, whole genome shotgun sequence.
ACCESSION   DAOSPL010000091 DAOSPL010000000
VERSION     DAOSPL010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15632_length_5305_cov_3.296952"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..2647
                     /locus_tag="PLQ54_02705"
     CDS             320..2647
                     /locus_tag="PLQ54_02705"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOS92198.1"
                     /translation="MRLRFRSLAAKLSFAFIAIAVLCVALQGALAVRATRPEVTAMGR
                     ENLVAITNGVLAMCQAQQDLLNQVLDFDLRLAESTLAQYGRITEGPKVTLTVADQTTQ
                     ATTTVTVPTWRAGAQPVHDDWTIVDTVQRLAPEGTTCIIFQRTEPGLLRVSTNVKKGD
                     GSRAVGTYIPASSPVAQTVLRGQTYQGIANVMGKPYVALYKPLKGEAGEVIGALYVGV
                     PQESVTSLRRAIMDIKVGDTGYVYVLDSKGNYVISKDGKRDGENILGAKDANGREFIK
                     EIVDPESQARKTGWIEYPWQNEGEKAARVKIVRLAYFEPWNWVIGAGSYVDEFQKPVA
                     AVTRSILKSSAAALAVAAVVGLWIAFGVAKPIRRVAERLEAISGGGADLTQELEVLSV
                     DETGRLAAAFNRFVAGLAQMVRDIREATDQVSHGAQTTASSAEAAACGVQEIATTAGS
                     VAEGAAQQRASLTEASRLVGEQDSALSSLAGSASALVQQVREASLSLQQMSQAVQSAA
                     DQAQSVAQAAERAHEAAQRGGTMVRSSLQGMERTGASAEESMRRIEHLAEKSRAIGDI
                     VDVIDDVSEQTNLLALNAAIEAARAGEHGKGFAVVADEVRKLAERATHSADEIRSLIR
                     EVQNGVAEAVECQRAGTREVAEGLALASQAGEAIDEIVGSVADAAEAVQQISAATEQL
                     LAQCEETRALASEMEAVSERSQEMLAMVADVASRVRSSTEGACQLAESAAAAAEQSSA
                     ATEELAAGIEEMTAITQEVSSAAERVKDMVSRFRV"
     gene            2841..5237
                     /locus_tag="PLQ54_02710"
     CDS             2841..5237
                     /locus_tag="PLQ54_02710"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOS92199.1"
                     /translation="MKMIPRVWDHIAANGWEGLPVRRGLRSCGLAAKLLVAFVVVGAL
                     CAAAEGVFALRTVRPEVASTARQNLEAITRGVLAMCQVQQDLLSKTLDNDLRITKRII
                     AEYGGNITEGPQTTLQAIDQVTGQSTMVQVPTWRVGPQPIHGDSTIVDRVRDLTADET
                     TCTIFQRTEPGLLWVSTNVKRNDGTRAVGTYIPVSSPVTQAVLRGETYRGIANVMGKP
                     YVAVYEPLRGPAGQVIGALYVGVPQDSVTSLRQGIMDIKVGATGYAFVLDSQGNYVVS
                     AGGKRDGENILETKDATGRPFIKEICDPQSEARRTGWIRYAWKNDGEDKARMKLVRLV
                     HFEPWDWIIGAGSYEDEINSSVHAVSRSLVISAVFGLLCASLLGGWIARRVVQPIRRV
                     LERLEAISGGGADLTQQIEVSSRDEVGRLAEAFNRFVAGLAQMVRDIRDATVQVSSGC
                     QTTAASAEGAASGVQEIASSSGSVAEGAARQREALIEATQLVGEQDQAVASLVDSSCQ
                     LVAQVREAALSLKQMGQAVQSAAAQAEEVGRSASAAQEAALRGGAMVRASLEGMERMG
                     ASAEESTRRIERLAEKSGAIGDIVDVIDDVSEQTNLLALNAAIEAARAGEHGRGFAVV
                     ADEVRKLAERATQSAGEIRSLIEEVQRGVAEAVECQRSGATEMAEGLRLATQAGEAID
                     EIVASVSDAASAVQQISAATEQLLAQCEQTRGITSQVESLSERSQQILAAVAQVASRV
                     RASTEDAFGLAEGAAAAAEQSSAATEELAAGIEEMTAVSQEVSAAAERVQAMVAHFRV
                     "
BASE COUNT          929 a         1775 c         1807 g          794 t
ORIGIN      
        1 agatctccga gctccggaga acccgcgact acgaggtgct cgagatgtac atcgtccgcc
       61 agatcatggg cggctaggtc aacacgacac ccgggggccg gccgttcgcc cggccggccc
      121 tcttccgctc tgctcccgcc gccccaccag aacgcattac atcgcctaca ctgtcaaatc
      181 tgcgtcaagt ccatgcaact tgcatacaca acccgtcccc tctgctttcg caccatggta
      241 tggattcctc cgcgcccaca gccgaagcgg gcgctcgtgc gtgggggctt accatgaagt
      301 cgtgggggag gttgatgtgt tgcgtcttcg ctttcggagc ctggctgcca agctctcctt
      361 cgcgttcatc gcgatcgcag tcctgtgtgt cgctctgcag ggcgcgctcg cggtccgcgc
      421 gacgcggccc gaagtcacgg ccatgggccg cgaaaacctg gttgccatca ccaacggcgt
      481 tctggcgatg tgccaagccc agcaggatct gctcaaccag gtgctcgact tcgacctccg
      541 cttggccgag agcaccctgg cccagtacgg ccgcatcacc gaagggccca aggtcactct
      601 gacggtagcc gaccagacca cccaggcgac gaccaccgtc accgtaccga cgtggagggc
      661 gggggcacag ccggttcacg atgactggac catcgtcgac accgtccagc ggctggcccc
      721 tgaaggcaca acatgcatca tcttccagcg caccgaaccg ggtctgctgc gcgtttcgac
      781 caatgtgaag aagggcgacg ggtcccgcgc cgtcggcacc tacatccccg cgtccagccc
      841 ggtggcgcag accgtcctcc gggggcagac ctaccagggc atcgccaacg tgatgggcaa
      901 gccctacgtg gcgctctaca agccgctgaa aggtgaggcc ggggaggtca tcggtgcgct
      961 gtacgtgggc gtcccccagg agagcgtcac gtcgctgcgg cgggccatca tggacatcaa
     1021 agtcggtgac accggctacg tgtacgtcct ggacagcaag ggcaactacg tcatcagcaa
     1081 agatggcaaa cgggacggcg agaacattct cggagccaag gatgccaacg gccgcgagtt
     1141 catcaaggag atcgtcgacc cggagagcca ggcgcggaag acgggctgga tcgagtaccc
     1201 ctggcagaac gagggggaga aggccgcgcg cgtgaagatt gtacgtctcg cctacttcga
     1261 gccatggaac tgggtcatcg gtgccgggag ctacgtggat gagttccaaa agcccgtggc
     1321 cgcggtgacc cgctcgatcc tgaagagctc cgccgctgcg ctggccgtcg cggccgtggt
     1381 tggcctatgg atcgcgttcg gcgtggcgaa gcccatccgg cgcgtggctg agcggcttga
     1441 agccatctcc ggtggagggg ccgacctgac tcaggagctc gaggtgctct ccgtcgacga
     1501 gaccggccgt ctcgccgccg ccttcaaccg cttcgtcgcc ggcctggctc agatggtacg
     1561 ggacatccgc gaggccaccg accaggtctc gcacggagcc cagaccaccg cgagcagtgc
     1621 ggaggccgcg gcatgtggtg tgcaggagat cgccaccacc gcgggcagcg tggccgaggg
     1681 ggccgcgcag cagcgcgcaa gcctcaccga agcatcccgg ctcgtgggcg agcaggacag
     1741 cgccctgtcc tccctggccg ggtccgccag cgcgctggtc cagcaggtcc gcgaggcttc
     1801 cctgagcctg cagcagatga gtcaggcggt gcagagcgcc gccgaccagg ctcagtcggt
     1861 cgcccaggcg gctgagcgcg cgcacgaggc ggcacagcga gggggtacca tggtccgctc
     1921 gtccctccag ggcatggaga ggaccggggc gtcggcagag gagtccatgc gccgcatcga
     1981 acacctcgct gagaaatcgc gcgccatcgg cgacattgtt gacgtcatcg acgacgtgtc
     2041 ggagcagacg aacctgctcg ctctgaacgc tgccatcgag gccgcccgcg ccggagagca
     2101 tggcaagggc ttcgccgtcg tggccgacga ggtccgcaag ctggccgaga gagccactca
     2161 ctcggccgac gagatccgct cgctgatccg cgaggtccag aacggcgtgg ccgaggccgt
     2221 cgaatgccag cgggccggca cccgcgaagt cgccgagggg ctcgcgttgg cgtcccaggc
     2281 cggcgaggcg attgatgaga tcgtcgggtc cgtggccgat gccgccgaag cggtacagca
     2341 gatcagcgcg gccaccgagc agttgctcgc ccagtgcgag gagactcgcg ccctggcctc
     2401 ggagatggag gccgtcagcg agcggagcca ggaaatgctg gccatggtcg ccgatgtggc
     2461 gtcccgcgtg cgttccagca ccgagggcgc ctgccaactc gccgagagtg ctgcggcggc
     2521 cgccgagcag tcctccgccg ccaccgagga actcgctgcc ggcatcgagg agatgaccgc
     2581 catcacccag gaggtctcct cggccgccga gcgggtcaag gatatggtct cgcggttccg
     2641 cgtctagctc gtccgtccga ccggaatctg tcccggcggc agcgatcagc cacgagatac
     2701 ccggcgcgtc gctgccgcca ttggtatgta cgtacccgtc tgaaccccgg cgacggtccg
     2761 gccccgagcg cgagcgtcac agagacctaa cataccagcc accccacttc atcccatagc
     2821 tccccgatgg aacaatgtct atgaagatga tcccacgtgt gtgggaccac atcgccgcga
     2881 acggatggga gggtcttcct gtgcggcgag gcctgcggtc ttgcggcctg gctgccaagc
     2941 tgcttgtcgc gttcgtcgtg gtcggcgccc tgtgcgctgc cgcagagggg gtgttcgctc
     3001 ttcgaacggt tcggcccgaa gtcgccagta cggcccggca gaatctggag gccatcactc
     3061 gaggggtcct ggctatgtgc caggtccagc aggatctact gagcaagact ctggataatg
     3121 acctgcgcat cacaaagcgc atcatcgccg agtacggggg caacatcacc gaaggtcccc
     3181 agaccacact ccaggcgatc gaccaggtta ccggtcagtc caccatggtt caggtaccga
     3241 cttggcgcgt tggtccgcag ccgatccatg gggactccac catcgtcgac cgcgtccgcg
     3301 acctcacggc cgacgagacg acctgcacca tcttccagcg taccgagccg ggcctcctct
     3361 gggtatcgac caatgtcaag agaaacgacg gcactcgcgc ggtgggcacc tacattccgg
     3421 tgtccagtcc cgtgacccag gccgtgctgc ggggggagac gtaccggggc atcgccaacg
     3481 tgatgggcaa gccctacgtg gccgtctatg agccgctcag ggggccggcg ggccaagtca
     3541 tcggcgccct ctacgtcggc gtgcctcagg acagcgtcac gtccttgcgg cagggcatca
     3601 tggacatcaa ggtcggcgcg accggatacg cgttcgtact ggacagccag ggtaactacg
     3661 tcgtgagcgc gggtggaaag cgtgacggcg agaacatcct cgaaaccaag gatgccaccg
     3721 gtcgcccgtt catcaaggag atctgcgacc cgcagagcga ggcgcgtcgc accggctgga
     3781 tccgctacgc ctggaagaac gacggtgaag acaaagctcg catgaagctg gtgcggctcg
     3841 tccacttcga gccatgggac tggatcatcg gtgcgggcag ctacgaggac gagatcaact
     3901 catccgtcca tgccgtctcc cggtccctgg tcatcagcgc cgtgttcggg ttgctgtgtg
     3961 cctcgttgct cggcggatgg atagcgcgac gagtcgtgca gcccatccgg cgagtcctgg
     4021 agcggctcga ggccatttcg ggcggcggag ccgatctcac ccagcagatc gaggtctcga
     4081 gccgcgatga ggtcggccgc ctggccgagg cgttcaaccg cttcgtcgcg gggctggcac
     4141 agatggtgcg tgacatccgc gacgccaccg tacaggtctc cagcgggtgt caaacgaccg
     4201 cggccagcgc cgagggtgcc gcgtcgggcg tccaggagat tgcctcgtca tcgggcagtg
     4261 tggccgaggg ggcggcccgt cagcgcgagg ccctcatcga ggcaacccag ctggtcggcg
     4321 agcaagacca ggccgtggca tcactggtgg actcctcctg ccagctggtg gcccaggtcc
     4381 gcgaggccgc gctgagcctg aagcagatgg gccaggcggt ccaaagcgct gccgcgcagg
     4441 ctgaggaagt gggccgctcg gccagtgccg cccaggaagc tgccctgcgc ggcggggcta
     4501 tggtccgcgc atcgctcgag ggcatggagc ggatgggtgc ctccgccgag gagtcgacgc
     4561 ggcgcatcga gcgcctcgcc gagaagtccg gcgccatcgg cgacatcgtc gatgtgatcg
     4621 atgacgtgtc ggagcagacc aacctgctgg ccctcaacgc ggccattgag gccgcgcggg
     4681 cgggtgaaca cggccgaggc ttcgccgtgg tggccgatga ggtccgcaag ctcgccgaac
     4741 gggcgaccca gtcggccggc gagatccggt ccctgatcga ggaggtgcaa cgcggcgtcg
     4801 ccgaggccgt cgagtgccag cgctcgggcg ccaccgagat ggctgagggg ctacgcctgg
     4861 ccacgcaggc gggcgaggcc atcgacgaga tcgtggcatc cgtctcggac gccgccagtg
     4921 ccgttcagca gatcagtgcc gccaccgaac agcttctggc ccaatgcgag caaacgcggg
     4981 ggataacctc ccaggtcgag tccttgagcg agcgaagcca gcagatcctg gccgccgtcg
     5041 cccaagtcgc ctcgcgggtg cgcgccagca ctgaagacgc cttcgggctc gccgaaggcg
     5101 cggcggccgc cgccgaacaa tcgtcagcgg ccaccgagga gctggccgcg ggcatcgagg
     5161 agatgacggc cgtctctcag gaggtctcgg cggcggccga gcgggtccag gccatggtcg
     5221 cccatttccg cgtctgaccc caaagacgtc acgccacccc gacgatggcc acggccaggg
     5281 ccagctcggg cgcaccgagc agcgc
//
LOCUS       DAOSPL010000092         5258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_15821_length_5258_cov_3.842399, whole genome shotgun sequence.
ACCESSION   DAOSPL010000092 DAOSPL010000000
VERSION     DAOSPL010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15821_length_5258_cov_3.842399"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="PLQ54_02715"
     CDS             <1..862
                     /locus_tag="PLQ54_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOS92200.1"
                     /translation="VGGKQVGLPIGMIGKFWFYNKKLFADQGVTPPKTWQDLLDAAAK
                     FKAAGILPISYGSKDQWTIAHYVGTLNQRILDPAVTKKDYEPKTGEFTDPGYAEALQR
                     FADLLPYMTPTPNAVAHQQARDAFIAGKAAMMYLEGAEYNYFKGSNLDWGTFNFPSMP
                     GGKGDQSQLTGAPEGFMIAKNSKHPEEALKLFKFLLSESMGVEWTNQTGELTAIKSGV
                     DKSNAPASVKEMANVIAASSEMTPWLDNALDQRLVSTYLSETQLLLGGQKTPAQVMAA
                     VQATAKQVRG"
     gene            972..1853
                     /locus_tag="PLQ54_02720"
     CDS             972..1853
                     /locus_tag="PLQ54_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014305765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOS92201.1"
                     /translation="MRSAGERALDYLFLLPALIFLGVFILYPLAANVGFSFSEFTLGS
                     AEKTFVGLDNYRQLIADPIVWTAWTNNVLYALVSVLFQVFIAHILAATVLVVLPSRLA
                     VAARSFFFLPVLLSMTVVAILFTFIFDASDGLLNGLLQAVGLGQLAHPWLGDTTTAIF
                     AVISVSQWQSIGYTMLLFIVSMQNIPTELYEAAALDGAKLLRQYVSITLPQTREMIFV
                     VMVLTVSGAFTVFAEPYILTGGGPGNASQVLATYMYRVGFFQNEMGYASALATLIFVI
                     TLVLSVAQATLFRTGKE"
     gene            1859..2692
                     /locus_tag="PLQ54_02725"
     CDS             1859..2692
                     /locus_tag="PLQ54_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOS92202.1"
                     /translation="MRLPYVAGVRLLPGRLVLTAYALILAFPLLWMIVSSFKPTLEVY
                     GRPWSLPSTWRFDNYSKAWEMGVSQYFVNSLFVTAITTAAVLAVAALAAYSLVRLRNW
                     AARLTMLIVMGALVISPQVSIVPLYDLLGRFGLLDTYTAMILPYVAYRLPMAILLIRA
                     VFLEVPRELEEASRLDGCGSLRTFLNVYWPVSAGVLATAAILTIYYTWNEFLFALIFV
                     DSDNLRTIPAGLMAFRDALATDWGVLLAGLTIAAVPVVVVFLLGQRFLIAGITAGSVK
                     G"
     gene            2696..3568
                     /locus_tag="PLQ54_02730"
     CDS             2696..3568
                     /locus_tag="PLQ54_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOS92203.1"
                     /translation="MDTPRPIACIGDNVVDVFRDRRRAYPGGNAYNVAAYAALIHKAP
                     ASFTGIVGDDEFGDHIVATLRELDIDAEGIRRAHGPSGQALVDVARDGDRIFVGSNRG
                     GVQRSLSLRLTHEDEQRLAASRLIHTSIYSGIDHLLPRLAELAPVSYDFSEAPEMSAI
                     QTLLPHISVACFSGAHLGDKDRRALGRRVLAHGPEVALVTAGRAGAYAYTTAEEHHEA
                     IVPTSVVDALGAGDSFITGFLHHWTLGASVAAAMAEGARSGAAACRFEGAFGHPVAVS
                     AEQIAAITAGVDAR"
     gene            3626..4420
                     /locus_tag="PLQ54_02735"
     CDS             3626..4420
                     /locus_tag="PLQ54_02735"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOS92204.1"
                     /translation="MANSAPETEPANRQTPLFKVVRDALRRAIDEGEYDNGDLIPPEP
                     ALCELYGVSRITVRRAVAELTSEGLLEKVPGKGTYVRSSVFTSPSLVSLAGFGQGERF
                     RVGPKRLVLRSWEEKASDRLARLLGVRRGSPLYALERLMLDGERPLAIDTTRYVAELV
                     PGFLAHVRDDVSTFDLLANRYGVQLGGSAGELRVGYATAHQAELLGRVTNDPVLLVEK
                     VISTREEQPILLSEIAYNPRGVSLRFQVDSAEPLTRFRQHTRTAVS"
     gene            4474..4548
                     /locus_tag="PLQ54_02740"
                     /pseudo
     CDS             4474..4548
                     /locus_tag="PLQ54_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612336.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(5103..>5258)
                     /locus_tag="PLQ54_02745"
     CDS             complement(5103..>5258)
                     /locus_tag="PLQ54_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010924694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HOS92205.1"
                     /translation="ASVIRLVGMILTDTNDEWITDERRYLSEGSMALLYPDRDNGHVP
                     ALPAGTV"
BASE COUNT          920 a         1737 c         1626 g          975 t
ORIGIN      
        1 ggtcggcggc aagcaggtgg gcctgcccat cggcatgatc ggtaagttct ggttctacaa
       61 caagaagctc ttcgccgatc agggcgtcac tccgccgaag acgtggcagg acctgctgga
      121 cgcggcggcc aagttcaagg ccgcgggcat tctgccgatc tcctatggat ccaaagacca
      181 atggacgatc gcgcactacg tcggcacgct gaaccagcgc atcctcgacc cagccgttac
      241 caagaaggac tacgaaccga agaccggcga gttcacagac ccgggttacg ccgaggccct
      301 gcagcgcttc gccgatctgc tcccctacat gacgcccacg cccaacgccg tcgcgcacca
      361 gcaggcccgg gatgccttca tcgctggcaa ggcggccatg atgtacctgg agggggccga
      421 gtacaactac ttcaagggca gcaacctgga ttggggcacg ttcaacttcc cgagcatgcc
      481 cggggggaag ggtgatcagt cacagctgac gggggcgccc gaggggttca tgatcgcgaa
      541 gaactccaag caccctgaag aggcgctcaa gctgttcaag ttcctgttga gcgagagcat
      601 gggtgtggag tggacgaacc agaccggcga actcacggcg atcaagagcg gcgtggacaa
      661 gtccaacgct cccgcaagcg tgaaggagat ggcgaacgtg attgcggcct cgagcgagat
      721 gacgccgtgg ctcgacaacg ccctcgacca gcgccttgtg tcgacctacc tcagtgagac
      781 ccagcttctg ctcggcgggc agaagacgcc ggcccaggtc atggcggcgg tccaggctac
      841 cgccaagcag gttcgcggct gaatcgaact cccgtccggc ctcggccggg ccttcgggtc
      901 cggccgaggc tgcaccccgc gacgtgctcg gctcgtcgca tctctttgtc actccggcga
      961 aaggagacga catgcgaagt gccggcgaac gtgcgctcga ctacctgttc ctgttgcccg
     1021 cgctcatctt cctgggtgtc ttcatcctct atccgctggc cgcaaacgtc ggcttcagct
     1081 tcagcgagtt caccttgggc agcgccgaga agaccttcgt cggcctcgac aactatcgac
     1141 agctgatcgc cgacccgatc gtctggacgg cgtggacgaa caacgtcctg tacgccctgg
     1201 tgtcggtgct gttccaagtg ttcatcgcac acatccttgc cgccactgtg ctggtcgtgc
     1261 tcccgtcacg actcgccgtt gctgcccggt ccttcttctt tcttcccgtg ttgttgtcga
     1321 tgaccgtggt tgcgattctg ttcacgttca tcttcgacgc ctctgacggt ctgctcaacg
     1381 gcctgctcca agcggtcggg cttggacaat tggcgcaccc atggttgggc gacaccacaa
     1441 cggccatctt cgcggtgatc agcgtctcgc agtggcagag catcggctac acaatgctgc
     1501 tgttcatcgt gtcgatgcag aacatcccaa cagagctgta cgaggcggcc gcgctcgacg
     1561 gcgcgaaact gctgcgtcaa tacgtgagca tcaccttgcc gcaaacccgc gagatgatct
     1621 tcgtggtcat ggtgctgacc gtgagcggag cgttcacggt atttgccgag ccctacatcc
     1681 tcaccggcgg cgggcccggc aacgcgagcc aagtactcgc cacgtacatg taccgggtcg
     1741 gattcttcca gaatgagatg ggctacgcct ccgcgctggc cacgctcatc ttcgtcatca
     1801 ccttggtgct gtccgtcgcg caggcgacgt tgttccgcac cggtaaggag tgagcaacat
     1861 gcgacttccg tatgtcgccg gcgtccggct gctcccggga cgacttgtcc tgacggcgta
     1921 tgcgctcatc ctcgcgttcc cgctgctgtg gatgatcgtc agttcgttca agcccaccct
     1981 cgaggtctat ggacgaccat ggagcctgcc cagcacctgg cgcttcgaca actactcgaa
     2041 ggcctgggaa atgggcgtct cgcagtactt cgtgaacagc ctcttcgtca cggccatcac
     2101 cactgccgcc gtcttggccg tagcggccct cgccgcctac tccctggtgc gcctgcggaa
     2161 ctgggcggcg cggctgacga tgctgatcgt gatgggcgcg ctggtgatct caccccaggt
     2221 cagcatcgtc cccctctacg atctgctggg tcgtttcggt ctcctcgata cctacaccgc
     2281 gatgatcctg ccctacgtcg cctatcgact cccgatggcc atcctgctca tacgcgcggt
     2341 gttcctggag gtgccccgag aactcgaaga ggccagccgt ctcgacggct gcggctcgct
     2401 tcggaccttc ctcaacgtct actggcccgt cagtgcgggc gtcctcgcta cagccgccat
     2461 cctcacgatc tactacacgt ggaacgagtt cctcttcgcg ctgatcttcg tcgacagcga
     2521 caacctgcga accatcccgg ccggcctcat ggccttccgt gacgccttgg cgaccgattg
     2581 gggggtcctg ctggccggcc tcaccatcgc ggcggtgccc gtcgtggtcg tgttcctcct
     2641 ggggcaacgc ttcctgatcg ccggtatcac cgctggcagt gtgaaggggt aagccatgga
     2701 cacacctcgc ccaatcgcct gcatcggcga caacgtcgtc gacgtgtttc gggaccgtag
     2761 gcgcgcctat ccaggcggca acgcgtacaa cgtcgccgcc tacgccgccc tgatccacaa
     2821 agcccccgcc agcttcaccg gcatcgttgg cgacgacgag ttcggcgacc acatcgtcgc
     2881 tacccttcgc gaactggaca tcgacgccga ggggatccgc cgggctcatg ggccgtccgg
     2941 gcaggcgctg gttgacgtgg ctcgcgacgg ggaccgcatc ttcgtcggct cgaaccgcgg
     3001 cggcgtccag cgctcgctct cgctgcgcct gacgcacgaa gacgaacagc ggctggcggc
     3061 ctcacgattg atccacacat ccatctactc gggaatcgac caccttctgc cccgcctcgc
     3121 cgaacttgcg cctgtgagct acgacttctc ggaagcgccc gagatgagcg ccatccagac
     3181 cctgctaccg cacatctcag tcgcgtgctt ctccggtgcc cacctgggcg acaaggatcg
     3241 tcgggcactc ggacgccgcg tcctggcgca tggcccggag gtggcgctcg tcaccgcagg
     3301 acgcgcgggg gcgtacgcgt acaccacagc ggaggagcac catgaggcga tcgtcccgac
     3361 ctcggtagtg gacgcattgg gcgccggcga ctccttcatc actggcttcc tccaccactg
     3421 gacgctcggg gcatcggtgg cggcggcaat ggctgagggg gcgaggtccg gcgccgccgc
     3481 ctgccggttc gaaggcgcct tcggccaccc cgtcgccgta agcgcggaac agattgccgc
     3541 catcaccgcc ggggtcgacg cgcgctagca tcagcgcaac gcgcgctagc ctcggcgtga
     3601 gagttgagct ctgaaggagg atggggtggc caatagcgcg ccggagactg agcctgccaa
     3661 tcgtcagaca ccgctgttca aagtcgtgcg tgacgcgctc cgccgggcca tcgacgaggg
     3721 cgaatatgat aatggcgacc tgattccgcc cgaacccgcg ttgtgcgagc tttacggtgt
     3781 ctcacggatc accgtgagac gcgcggtcgc ggaactgacg tcggaagggc tgctagagaa
     3841 ggtccccggc aagggtacct acgtccggtc gagcgtcttc acctcgccct ccctggtatc
     3901 ccttgccggc ttcggtcagg gggagcgctt ccgagtgggg cccaagcgtc tggtgctacg
     3961 gagctgggaa gagaaggcta gtgatcgact cgcacgtctg cttggcgtac gtcgaggttc
     4021 ccccctctat gcgctcgaaa ggctcatgct cgacggcgag cgtccgcttg cgatcgacac
     4081 cactcggtac gtggcggaac tagtgccggg attcctcgca catgtgaggg acgatgtctc
     4141 gactttcgac cttctggcca accgctacgg agtgcagctg gggggctcgg ctggcgagct
     4201 ccgagtcggc tatgccacgg cgcaccaagc cgaactcctc ggccgtgtca cgaacgaccc
     4261 ggtgttgctg gtggagaagg tgatctcgac gagggaggag caacccatcc tcctgtcaga
     4321 gatcgcctac aacccgcgtg gcgtgtcgct gcgcttccag gttgacagtg ctgagccact
     4381 gacccgattc aggcagcaca cacggaccgc tgtgagctag caactagccg tcgctcaccc
     4441 tgactcgtgg gcgaaggtga gcccaccgcc tgccccgcgc tccagcacta caacaccgaa
     4501 cgccgtcaca gcgcactcgg aggacaacca ccgatcagcc gactgtagcc aacctgatgg
     4561 ccgggtatag cgaggtcggc ggcgccgccg catttcacgc tggccccgtg ggactcgcga
     4621 gctcgccgac cgcagacaca cctcatgcgc gagccgttca cgatcccgtc ctacgagggc
     4681 gacctatgcc aagctcccta agggctgagt gggctggtgc accggtatct gcaggtgggg
     4741 cctgagtacg cggccccagg ggacacctag aagaacagcc cggtcgtaca ctcgaagacg
     4801 ctgccacaag gtgatcaggt cctccttgca ggtagcgcac aaatcgaaca cggacggccg
     4861 aggcttagtc tcagtattag tctcattcct acccgttcag cggtgttcac gagaggtcgc
     4921 agtctcagtg aaacacctag tcgcggccat gtatggacat ccctgaacgg cactgtgacc
     4981 agttgtaaag cgggttgggt ttacgccctc cggagtgtga acctcgtgac ggcggacgat
     5041 gtggatccag agttcgagac agtgtcccgg gtggtggtgg actttcgagg ggtcctcggt
     5101 gtctacaccg tccctgccgg gagggcgggc acgtgcccat tatcgcggtc gggatacagc
     5161 agcgccatgg agccttcgga taggtagcgg cgctcatcag tgatccattc gtcgttggtg
     5221 tcggtcagga tcatgccgac gagccggatt acggaggc
//
LOCUS       DAOSPL010000093         5257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_15826_length_5257_cov_2.932526, whole genome shotgun sequence.
ACCESSION   DAOSPL010000093 DAOSPL010000000
VERSION     DAOSPL010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15826_length_5257_cov_2.932526"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1716)
                     /locus_tag="PLQ54_02750"
     CDS             complement(<1..1716)
                     /locus_tag="PLQ54_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92206.1"
                     /translation="MRPPLLCLLATFAATAWSQELPRYYAHEAVLDANGVIAPWYRRQ
                     NGPLDLRVRVAAETLKRYPWTERPRPAPHYVFSGAWGIAPDGTISIPPIDNWSNGDLG
                     QRAAYVLSGLVDYYRYSGDPAAISHIAITADALIDFCQTPADHPWPRFLVSVPTKGMP
                     YGVTDPHGFIQLDIVAEVGIGMVRAYELVGERRWLEAAKHWADLLVQHCDLRPGAPPW
                     PRYANPEDVPWEDHCTGGVVFMLAFFDELERVGYTGRRGELTRAREAGEAYLRDTLLP
                     RWTEDGTWGRNYWDWNDPVQAENVTEFAVRYMMEHPSRFPNWRNDCRNVLSLFLNRTS
                     VARESNGDVFSGAWAYPESSGCCGRSLWYGPFELAPIYAQYSELTGDEWAREMARRQL
                     ILATYDVHETGVVEDNIDGGPIVAGAWFKIAHPMALKHALNGMAWLPDLMGAARENHI
                     LRASAVVRHVQYGVGDIRYETAETIAPCVDVVRLAFEPAKVTADGVRLAARGDLAANG
                     FTTRRLECGDVILTIRHDGRKRVAILGPDPGQAADDTELTFEGPWRAANRAGDAGGTA
                     HAAAAA"
     gene            complement(1743..2672)
                     /locus_tag="PLQ54_02755"
     CDS             complement(1743..2672)
                     /locus_tag="PLQ54_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92207.1"
                     /translation="MSHIAYIIASPPGYEALAAAEARAVCPGSWVTPRCVVSDAGVDV
                     DRTAFVHYRIELLAHGHDYAGVCDLIGQLNLVSDRFRIEVHKHPRRTPMPRESVARDL
                     ANLITGQPDLAAPSERFVFWLEADTMLFGRIQQETANDWKQRMAKPFNVSAGIGSRMA
                     KACVNLVCSPGDRMLDPCCGSGTIPIEAALIGVRAFAFDLSWRMAHGTAANARHYGLD
                     IGVGIGDVRSLAGRFDAVVSNLPYGIAIRHSSGFYDEAIANIARLAPRCVLISNREVI
                     DVAERNGLRVDDVIRQGESDGKLPRFVHLLSRV"
     gene            complement(2718..4313)
                     /locus_tag="PLQ54_02760"
     CDS             complement(2718..4313)
                     /locus_tag="PLQ54_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOS92208.1"
                     /translation="MRVVTAAEMRELDRQTIDEVGLPGPVLMETAGRSVAHVAFALLG
                     QRRGARVAIACGRGNNGGDGFVAGRYLLNLGAAPECFVLAKRADITGDALLHLIAAER
                     SGLPIRELGPDGGGALALAVSSSDLTIDALLGTGLRGEVREPFWSGIESINRLARQVL
                     AVDVPSGLDADTGAVLGQAVRATATVTLGLPKVGLLQYPGAALCGDVKVADIGIPAAL
                     SAPRRGGLRLATADWVAGILPRRERDAHKGDAGRVMIAGGAVGLTGAPAMAARAAARM
                     GAGLVHVAVPASLHSIMEGLVLEPMSLPLPEAGPGTLGGEAAGHLLEAMRARPCHVLA
                     VGPGLGRHPGAVELVSVLVSGSSVPVVLDADALWAVAQAGEAATAWAAPVVLTPHPGE
                     LSVLLGVSVADIQRDRVAAARGAAQRFRAIVVLKGARTVTAHPDGTAVINATGNPGMA
                     SGGMGDVLTGAIAALIAQGVPLLEAAAAGVYVHGLAADLVAADGGCEAGLLASDVIEA
                     LPRARQHAAEGRELLPGRVPERV"
     gene            complement(4325..>5257)
                     /locus_tag="PLQ54_02765"
     CDS             complement(4325..>5257)
                     /locus_tag="PLQ54_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92209.1"
                     /translation="ALPSGQVAALDETGARRPIVSVPAPPTRLAAAGGAVVVGMADGR
                     VWCLADGEPQWEFACPAYRGQGSVTAVLASGDESAPLRATIGTAGGLVMGLDARGDPI
                     WARDLGLSSVRGLVALPSPIGGLLLCWSAEGVWALAPASGRAVWTEPADDRPLCGVSV
                     VPGGSDWWQAIVADAGGRIRALGPPQERQSVSAGVAWEANAGGVTTGVVPVVSGRQAR
                     LAVAATSGWLVRLELDGAEVGRTRLGSAIVGLAPVSGGRVLTLSADGVVHLVGWGGEV
                     VGRTELGRRWSGCCVAGGRVFGLGPEGLACLEPV"
BASE COUNT          888 a         1990 c         1679 g          700 t
ORIGIN      
        1 ggcggccgcc gccgcatggg ccgtccctcc cgcgtcgccc gcccggttcg ccgcgcgcca
       61 agggccctcg aacgtgagct cggtgtcatc ggcggcttgg ccaggatccg ggcccaggat
      121 cgcgacacgc tttcgtccat catggcgaat ggtcaggatc acatcgccgc actcgagacg
      181 cctggtcgtg aacccgttgg ccgcaaggtc accccgagcc gcaagcctca caccgtctgc
      241 ggtcaccttg gccggttcga aggcgagccg gacgacgtcg acacacggcg cgatggtctc
      301 ggccgtctca taccggatat cacccacgcc gtactggaca tgccgcacca ccgcgctcgc
      361 gcgcaggatg tggttctcgc gggccgcccc catcaggtcc gggagccagg ccatgccatt
      421 gagcgcatgc ttcagcgcca tgggatgggc gatcttgaac cacgcgcccg ccacgatggg
      481 cccaccatcg atgttgtcct cgaccacgcc cgtctcgtgg acgtcgtagg tcgccagaat
      541 gagctggcgc cgggccatct cgcgagccca ctcatctccg gtcagctcgg aatactgcgc
      601 atagatgggt gccagctcga aggggccgta ccatagcgac cggccgcagc agcccgacga
      661 ctccggatag gcccacgcgc ccgagaagac atcaccgttc gactctcggg ccacactggt
      721 gcggttgagg aagagcgaga gcacgttccg gcagtcgttc cgccagttcg ggaaccggct
      781 cgggtgctcc atcatgtacc gcaccgcgaa ctcggtaacg ttctccgcct gcactgggtc
      841 gttccagtcc caatagttac ggccccaggt accgtcctcg gtccaccgag gcagcagggt
      901 gtcgcggagg taggcctcgc cggcctcccg ggcgcgcgtt agctcccccc tccgaccggt
      961 gtaccccacg cgctctagct catcgaagaa cgccagcatg aacacgacgc cgcccgtgca
     1021 gtgatcctcc caaggcacat cctccgggtt ggcgtagcgc ggccacgggg gcgcgccggg
     1081 gcgcaggtcg cagtgctgca ccagaaggtc ggcccagtgc ttggcggcct cgagccagcg
     1141 cctctcaccc acaagctcgt aggcgcggac catgccaatg cccacctcag ccacgatgtc
     1201 gagctgaatg aagccgtgcg ggtcggtaac gccatacggc atgcccttgg tgggcacgct
     1261 caccaggaac ctcggccagg gatgatcagc cggtgtctgg cagaagtcga tgagcgcatc
     1321 ggccgtgatc gcgatgtggc tgatggctgc tgggtcgccc gagtagcggt agtagtccac
     1381 aagccccgag agtacgtagg cagcccgctg cccgaggtcg ccattggacc agttgtcgat
     1441 aggaggaatc gagatggtgc catccggcgc gatgccccac gctccgctga agacgtagtg
     1501 cggcgccggt ctgggcctct cggtccacgg atagcgcttc agcgtctcgg cggcgacgcg
     1561 gactctgaga tcgagcgggc cattctgccg gcggtaccag ggcgcgatga cgccgttcgc
     1621 gtcgagaacg gcctcgtggg cgtagtagcg cggcaactcc tgagaccagg cagtggcggc
     1681 gaaagtggcc agcaggcata acaggggggg cctcacgagg cgtcgcctcc cggtgagttc
     1741 gctcatacgc gactcagcag gtgaacgaac cgcggcaact tgccatcgga ctcgccctgc
     1801 cggatcacat catctacacg caggccgttc cgctcggcca cgtcaatcac ctcgcggttc
     1861 gagatgagga cgcagcgcgg tgccaggcga gcgatgttcg cgatggcctc atcgtagaag
     1921 cccgagctgt ggcggatcgc gatcccgtag ggcaggttcg acaccacggc gtcaaaccgg
     1981 cccgccaacg agcgcacatc gccgatgccc acaccgatgt ctagcccgta gtgccgtgcg
     2041 ttggccgcgg tgccgtgcgc catgcgccaa ctcaggtcga aagcaaatgc cctgacgccg
     2101 atcagggccg cctcgatcgg gatcgtgccc gacccgcagc aggggtccag catccggtcc
     2161 ccggggctgc agaccaggtt gacgcaggcc ttggccatcc gtgagccgat ccccgcgctg
     2221 acgttgaacg gcttggccat ccgctgcttc cagtcgttcg cggtctcctg ctggatccgg
     2281 ccgaacagca tggtgtccgc ttcgagccag aagacgaagc gttcgcttgg cgccgccaga
     2341 tccggctggc ctgtgatgag attggccagg tcccgcgcta cgctctcgcg cggcatcggc
     2401 gtccgccggg ggtgcttatg aacctcgatc cggaaccgat ccgacaccag attgagctgg
     2461 ccaatcaggt cacagactcc agcgtagtcg tgcccgtgcg ccaacagctc gatccgatag
     2521 tggacgaagg cggtgcggtc tacatcgacc ccggcatcgc tgaccacgca gcgcggcgtg
     2581 acccacgacc cgggacacac ggcgcgggcc tcggcggccg ccagcgcctc gtatcccggc
     2641 ggcgacgcga tgatgtaggc gatgtgagac acctgcggtc tccctagcgc gagcgcgcgc
     2701 aatgccggcc ggcatcttca cacgcgctcg ggcacccgcc cgggcaggag ctcacggccc
     2761 tcggccgcat gctgcctggc ccgcgggagc gcctcgatca cgtcgctcgc cagcaggccg
     2821 gcctcgcagc cgccatcggc cgccaccagg tcggccgcca ggccgtgcac gtacacaccg
     2881 gcggctgcgg cctccaatag gggaacgccc tgggcgatca gagccgcgat agctccggtc
     2941 agcacatcgc ccataccccc tgaagccatg cctgggttac ccgtcgcgtt gatcaccgca
     3001 gtcccgtcgg ggtgggccgt cacggtgcgc gcgcccttga gtaccacaat ggcacggaac
     3061 cgctgagccg cgccgcgcgc cgccgcgaca cggtcgcgct ggatgtcagc cacggacacg
     3121 ccgagcagca ccgagagctc gcctgggtgg ggcgtcagaa cgaccggcgc cgcccacgca
     3181 gtcgccgcct ctccggcctg cgccaccgcc cacaacgcgt cggcgtccaa caccacggga
     3241 acggacgatc cgctgaccaa caccgagacc aactcgacgg cacccggatg ccggcccagc
     3301 cccggtccaa cggcgagcac atgacaggga cgtgcccgca tcgcctcgag cagatggccc
     3361 gcggcctcgc cccccagtgt cccgggcccg gcctcgggga gtggaagcga catgggttcg
     3421 agcacgagac cctccatgat cgagtggagg ctcgcaggga ccgcgacgtg caccagcccc
     3481 gcgcccatcc gcgctgcggc acgcgccgcc atggccggcg cgcccgtgag gcccaccgcg
     3541 cccccggcga tcatcacacg gccagcatcg cccttgtgcg cgtcgcgctc gcgacgcgga
     3601 aggatgcccg cgacccaatc ggcagttgcc aaccgcaggc cgccccggcg aggtgccgac
     3661 agcgctgcgg gaatgccgat gtcggcgacc ttcacgtcgc cacagagagc ggccccgggg
     3721 tactgcagca ggccgacctt gggcaggcca agcgtcaccg ttgccgtggc ccggaccgcc
     3781 tgccccagca cggcccccgt gtcggcgtcg agccctgacg ggacgtcgac cgccaacacc
     3841 tgtcgcgcca gccggttgat gctctcaata cccgaccaaa acggctcgcg cacctcgccc
     3901 ctcagtccag tgcctagcag tgcatcaatc gtcaggtcac tcgaggatac ggccagcgcc
     3961 agggctccgc ctccgtcggg tccgagctca cggatgggca accccgaccg ttcggccgcg
     4021 atcaggtgca ggagcgcgtc gccggtgatg tcagcccgtt tcgccagcac gaagcactcc
     4081 ggcgccgcac ccaggttgag gaggtagcgt cccgcgacga agccatcccc gccgttgttg
     4141 cctcgcccgc acgcgatcgc cacccgcgcg ccgcgccgct gccccagcag cgcaaacgcc
     4201 acgtgggcca ccgaccggcc agccgtctcc atcagcacgg gcccgggcag tcccacctca
     4261 tcgatggtct gccggtcgag ctcgcgcatc tcagccgcgg tgacaaccct cacggccagg
     4321 ccccctacac cggttcgagg cacgccaggc cctctggacc tagcccgaac acccgtccgc
     4381 cggctacaca acacccggac caacgccggc ccagctcggt acggccaacg acctcgccgc
     4441 cccagccgac caggtgaacc acgccatcgg ccgatagagt caggacccgt ccgccagaca
     4501 ccggcgcaag gcccacgatg gccgaaccca gtcgcgtgcg gcccacctca gcgccatcga
     4561 gctccaggcg aaccagccag ccgctggtgg cggccaccgc cagccgcgcc tgccgccccg
     4621 agacgaccgg gacgacaccg gtcgtcacgc ctcccgcatt cgcctcccag gcgactccgg
     4681 ccgagacgga ttgcctctcc tgggggggcc cgagagcccg aatgcgacca cccgcatcgg
     4741 ccacgatggc ctgccaccaa tcggacccac ccggcaccac cgagacgcca cagagcggcc
     4801 gatcgtcagc cggctcggtc cataccgccc ggccggacgc cggagcgagc gcccacacgc
     4861 cctcagcgga ccagcagagc agaaggcccc cgatgggcga cgggagggca accaggccgc
     4921 ggaccgagga caaacccaga tccctcgccc agatcggatc gccccgtgcg tcgagaccca
     4981 tcaccagacc gcccgcggtc ccgatggtcg cccgcaacgg agcggactcg tcacccgatg
     5041 cgagcacggc cgtgaccgag ccctgacccc ggtaggctgg acatgcgaac tcccactgtg
     5101 gctcgccatc ggccaggcac cacacgcgcc catcggccat cccgaccacc acagcgcctc
     5161 cggcggctgc cagccgggtt gggggggccg gcaccgaaac aatcgggcgc cgcgccccgg
     5221 tctcgtccag ggccgccacc tgtcccgacg gcagcgc
//
LOCUS       DAOSPL010000094         5243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_15870_length_5243_cov_3.563416, whole genome shotgun sequence.
ACCESSION   DAOSPL010000094 DAOSPL010000000
VERSION     DAOSPL010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15870_length_5243_cov_3.563416"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PLQ54_02770"
     CDS             complement(<1..790)
                     /locus_tag="PLQ54_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92210.1"
                     /translation="MPGWIILSVGLGTAVLVALSAWPLTTWAYGDWVALSATTRESLV
                     YAGPWAAGWCAWTAGRYLGPRSLLCSPAASRSGTPVVRAHLAWLCGAAVTGHVLGLLP
                     VIVSTAGRATAGGLDWLVDLGSTGVLLTFCALGYLTGCVIPRVASVIAAVVITFVLIL
                     FVDTWGLALAPLWLSTPAAGQQEAPVVAAFRAVFYLGFAVILAITAGRWVADRATVRT
                     PATYATLTLLMLPVLVGATARNAAPPALRAESNPSAVCTEVQGVS"
     gene            complement(804..1388)
                     /locus_tag="PLQ54_02775"
     CDS             complement(804..1388)
                     /locus_tag="PLQ54_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS92211.1"
                     /translation="MIGGHDIHTRGGRHAARALLGWLPQRFDLAGGMTALETVAYAAW
                     ANGCDAKAADHRAAEALGLVDMSGLASTRVRRLSGGQRQRLGLAAAMAHDPSVLLLDE
                     PTVGLDPEQRVKFRASIRRAASRRTIVLSSHLLEDIAHVCDRVAVLHQGEIRFTGTPT
                     ELAALANGDVDTSDDGLTNPLEAGYLHALRSDDA"
     gene            complement(1581..1907)
                     /locus_tag="PLQ54_02780"
     CDS             complement(1581..1907)
                     /locus_tag="PLQ54_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92212.1"
                     /translation="MALPLAITFTPAWAASTASCDVFAYTPFAGGDEVGGTGGRSGCT
                     STVSVKTRLKYDRFGPDPTTAFRGGRVTNITWTAYGCSGRQSYYTNTSTDSGQVSGSL
                     NRTITC"
     gene            complement(2295..2645)
                     /locus_tag="PLQ54_02785"
     CDS             complement(2295..2645)
                     /locus_tag="PLQ54_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92213.1"
                     /translation="MTSGDFLDALGRDVPEAQSVVAEHLEDNDVLLLHLLTGDLRRFA
                     FHSFEMGRRDVLERLLALVDLALREGTDHVRGAMTVSFVGNTEWWDPAMQPFIDTWPA
                     GLRAEVHRQRSQNQ"
     gene            2955..3299
                     /locus_tag="PLQ54_02790"
     CDS             2955..3299
                     /locus_tag="PLQ54_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011208140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOS92214.1"
                     /translation="MSQFGLVVRFALRPGRAEAFDALMRDTVAGIAAHEPRTLAYAVH
                     ALAGEPDVRIFYELYTSEDALAEHESQTTTRYFLDRVDDYVTSVDVQRLRLVTATGIA
                     DLPAGAERPAPS"
     gene            complement(3377..3709)
                     /locus_tag="PLQ54_02795"
     CDS             complement(3377..3709)
                     /locus_tag="PLQ54_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOS92215.1"
                     /translation="MLRIGAVVLNVADIGDSGSFWSSALGYAPQPRNPAFLVPTQGHG
                     PRVHLDQDDRTHLDLWVDTEEEQLAEVGRLISLGASRVDWQYPDDADFVVLADPGGTL
                     FCVIDLSK"
     gene            3936..4559
                     /locus_tag="PLQ54_02800"
     CDS             3936..4559
                     /locus_tag="PLQ54_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92216.1"
                     /translation="MGRISALVGLAAAVLVVSGCAVPWSRTTTANKPEVNASELTDAG
                     GLPCPQDLPVGDDPSGHGFGTQEVADELPTLLEPQLAWVCQYNTFDVGTTPGGGTTYG
                     WRRSGQPKPVPAGDLPDLYAALNDLAPADRSGGCDADLGPRWMVVYSHAGDLTGVVVD
                     DYGCRDVRLTDNPHVTPPGAGDQDGTVGGLLDGGAAILHALGVGRSN"
     gene            4695..5018
                     /locus_tag="PLQ54_02805"
     CDS             4695..5018
                     /locus_tag="PLQ54_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92217.1"
                     /translation="MRGRPGPGALIGLVVGVVLVAVVLWLPRALADDFYEFSDPLIYM
                     GLPMIVISASVGALVGVPAPVSGEAPSDSARRSSWPSRAAVAVVSVAAVFIALWFFLT
                     ATGSI"
BASE COUNT          871 a         1750 c         1798 g          824 t
ORIGIN      
        1 ccgagactcc ttggacctcg gtgcacacag cggatgggtt ggattcggct cttagagcgg
       61 gcggggccgc gttccgtgcg gtggctccga ccaggacagg cagcatcaac agtgtgagcg
      121 tggcgtaggt cgctggcgtg cgcacggtgg cgcggtcggc cacccagcgt cccgccgtga
      181 tggccaggat gaccgcgaac ccgaggtaga agacggcgcg gaacgcggcc accacggggg
      241 cttcctgttg gccggccgca ggtgtcgaaa gccacagcgg ggcgagggcg aggccccacg
      301 tgtcgacgaa gagaatcagc acgaaggtga tgaccacagc ggcgatgacg gaggcgacgc
      361 gggggatgac gcatcccgtg aggtagccga gcgcgcagaa ggtcagcaga acgccggtag
      421 acccaaggtc gacgagccag tcgaggcctc ccgcggtggc tcgaccggcc gtcgagacga
      481 tgaccggcag cagtccgagg acgtgaccgg tgacggcggc tccgcacagc caggcaaggt
      541 gggcccggac caccggtgtg cccgatctgg aggccgccgg cgagcagagc agggatcgcg
      601 ggccgaggta tcggccggcg gtccaggcac accacccggc ggcccagggg ccggcgtaga
      661 ccaacgactc tctcgtcgtc gcggagagcg cgacccagtc gccgtaggcc caagtggtca
      721 gcggccacgc actgagggcg acgaggacgg ccgtgcccag accaaccgac aggatgatcc
      781 aaccaggcac cgccagaggt cgcctaggca tcgtcggacc ggagcgcgtg caggtagccc
      841 gcctccaagg ggttggtcag gccatcatcg gaggtgtcga catcaccgtt cgcgagggca
      901 gccagctcgg tcggggtgcc cgtgaacctg atctcacctt ggtgcaggac ggcgacgcga
      961 tcacacacgt gtgcgatgtc ttcgaggaga tggctggaca gcacgatagt ccggcggctg
     1021 gcggccctgc ggatggaggc acggaacttg acgcgttgtt cagggtccaa cccgaccgtg
     1081 ggctcatcca gaaggagaac cgacggatcg tgcgccatgg cggcggcgag cccgaggcgc
     1141 tggcgctggc caccggagag ccggcggacg cgagtcgaag ccaacccaga catgtcgacc
     1201 aaaccaaggg cctcggcggc ccggtggtcc gcggctttgg cgtcgcatcc gttggcccat
     1261 gccgcgtagg ccaccgtctc tagagccgtc atccctccgg cgaggtcgaa ccgctgcggt
     1321 agccagccga gcagggcacg cgcggcgtgg cggccgccgc gtgtgtggat gtcgtgacca
     1381 ccgatcaaga cgcttccgct gtagggcgga gtacggtcgc cagcacggac agcagcgtgg
     1441 atttaccggc cccgttaggt ccgaggagcc cgagcacccc gtgtccggcg tgaagggtga
     1501 ctccatggag cgccagttgg cggctcccgt agctgaccga gacctcgcgt gcgtcgatgg
     1561 cgggttggga gtccgtcatc tcagcaggtg atggtgcggt tcagcgaccc cgagacctgt
     1621 cctgaatcgg tgctcgtgtt cgtgtagtac gactgacgac cggaacaccc gtaggcggtc
     1681 caggtgatgt tggtgacccg cccgccccgg aaagccgttg tgggatcggg cccgaaccgg
     1741 tcgtacttca gccgggtttt gacgctgacg gttgaggtgc accccgacct gccgcccgtt
     1801 ccgcccacct cgtccccgcc ggcgaagggg gtgtacgcga agacgtcgca cgaagcggta
     1861 ctcgcggccc atgcaggcgt gaaggttatg gccagcggga gagccacagc ggatgcgacg
     1921 gccgggctga tgactcgatt cgaaattgga ttgccccgtt cgtgagcgtg cctcggaggc
     1981 tactcccgtc gatcggctcc cgcatccgag aacgattccg ctaccccaga cagcgcgagc
     2041 ggcagagcga cgcctctccg gcccactgac actcacggag tgccatcgcc ggacctgtgc
     2101 gcggcacgcc cgtcttgtat tacccaagta gcgagtatca cggtggtggt gcgccgcgtc
     2161 agcgccaact cgatgggagc ggccagcccg tcgatgcccc agactcgcat gagccgttcg
     2221 ggtagcacgc acgaccagcc gctcctcggt gcgaggcgat taccggattt gccgctcacc
     2281 gtgcgcattg gggactactg attctggctc cgctggcgat ggacctcggc ccgcagcccc
     2341 gctggccagg tgtcgatgaa gggctgcatc gcggggtccc accactcggt gttccccaca
     2401 aacgacaccg tcatggcgcc ccgaacgtga tctgttccct cgcgaagcgc aaggtctaca
     2461 agagccagaa ggcgctccag aacgtctcgc cgccccattt cgaaggagtg gaacgcgaag
     2521 cgtcggaggt cacccgtgag caggtgaaga aggaggacat cgttgtcctc cagatgctcg
     2581 gcgaccacgg actgggcctc cggcacgtcc ctgccgagcg cgtcgagaaa gtcccccgat
     2641 gtcatggcca cagccgcatg atgcccgaag aagcgcacgc acatgccgcg gatcgcgcgg
     2701 gtgttctcag cgctgatctc cgctcccgca tgggggtgac ccggcgctag gcgggagtcg
     2761 ccttcgacgg ctgaacgtag gtgcgcttcg gcgtgccgaa cctgagggac cgcagcccgc
     2821 gcgacggccg acctcggtcc tcgccggggg tgttcagcct tgtgggacgc cgacctggac
     2881 cgcgcggcga cctccgtcct caccgtgtcg cagtccccga cgtgggatgg tcggctcgta
     2941 ccgaggagga gcagatgtcc cagttcggct tggtggtccg cttcgcgctc aggcccgggc
     3001 gagccgaggc gttcgacgcg ttgatgcgcg acacggtggc gggcatcgcc gcccacgagc
     3061 cccgcacgtt ggcctacgcg gtgcacgcgc tcgctggtga gcccgacgtc cggatcttct
     3121 acgagctcta caccagcgaa gatgccctgg ccgagcacga gtcgcagacc acgacccggt
     3181 acttcctcga ccgcgtcgac gactacgtca cgagcgtcga cgtccagcga ctgcgcctgg
     3241 tgaccgcaac cggcatcgcg gacctcccgg ccggcgccga gcggccggcg cccagctgaa
     3301 cccgcatccg atcttgcacc cgaccgcagc gagcaggacg tccgtccgtg ctgcgggtaa
     3361 agaaggtctc gtcgccctac ttgctcaggt cgatgacgca gaagagcgtg ccgccaggat
     3421 cggcaagcac gacaaaatcg gcgtcgtcgg ggtactgcca atccactcgg ctggcgccga
     3481 gcgagatgag gcgcccgacc tcggccagct gctcttcctc cgtgtcgacc cagagatcga
     3541 ggtgagtgcg gtcgtcctgg tcgaggtgta ctcgcggacc atggccctgc gtgggcacca
     3601 agaatgctgg gttgcgtggt tgcggcgcat accccagtgc gctggaccag aaagagccag
     3661 agtcaccgat gtccgcgacg ttcaggacaa cggctccaat gcgcagcatg gactgagagt
     3721 ggcacggcga cggtcttcag acaacggcgt atgccgaggt catcagggtc cggcctcgtc
     3781 cgcacaccag cgtgagggct tatccggatt tgccgccgat cgtgccgatg agcgcgcgct
     3841 accccttgga accgttacct tgtggaatcc gtcgaatcgt catgcggccg cggaccgcgc
     3901 gcctctccag tgccgttatc ccccgacatc ggaccgtggg ccggattagt gcgttggtcg
     3961 gcctggcggc cgcagtcctc gtcgtcagtg gatgcgctgt gccttggtcg cgcaccacga
     4021 ccgcgaacaa gccagaagtc aacgccagcg aacttaccga cgctggtggc ctgccgtgcc
     4081 cacaggacct gccagtcggc gacgacccct ctgggcatgg cttcggcaca caggaagtcg
     4141 cggacgagct cccaacgctt ctcgagccgc agctggcatg ggtgtgccag tacaacacct
     4201 tcgacgttgg cacgacgcca ggtggtggca ccacgtacgg atggcgtcgc tccgggcagc
     4261 ccaagcccgt tcctgctggt gacctgcccg acctctatgc cgctctgaac gaccttgccc
     4321 cggctgaccg tagcggcgga tgcgacgccg acctcggccc acgttggatg gtcgtgtaca
     4381 gccacgccgg cgatctcacc ggcgtggttg tcgacgacta cggctgccgc gacgtccgcc
     4441 tcaccgacaa ccctcacgtc accccgcctg gagcgggcga ccaagatggg acggtcggcg
     4501 gcctcctcga cggaggggcc gcgatccttc atgccctcgg ggttgggcgc tcgaactgac
     4561 ccgggccccc ggaccacgtt tcccaactcc gcgctacatc ccaagagcga gatgggacgt
     4621 ccggaactgc cgcgacccgt gtatccatgg tcggacaggt tgccagggtg ggccgccgcg
     4681 gccacactcg ggccatgagg ggtcgaccag gtccgggggc gctcatcggc ctggtggtcg
     4741 gtgttgtgct cgtcgccgtg gtgctgtggc tcccgcgagc gctcgccgat gacttctacg
     4801 agttcagtga cccactcatc tacatgggcc tgccgatgat cgtgatcagc gcctcggtgg
     4861 gtgccctcgt cggtgtaccg gcgccggtgt ccggcgaggc gccatcagac tcggctcgac
     4921 gctcctcgtg gcccagccgg gctgccgtcg cggtcgtatc agtggccgcg gtgttcattg
     4981 cgttgtggtt cttcctgacg gccactggat cgatctgaca cctgccacgg ctgtccgtgc
     5041 gccagacgca gaacccgtcc cgcgactgct cgacgtgcgg aactgccgcg cagggtgcga
     5101 accgtgtcga tgtcccgtcg gtcaactcct gaagaagaag ccgctgcggt acgtccacgt
     5161 tccggagagg tcttcgtggc cagagctgtc caaggactcc tgcaggtgga ccgtcatcaa
     5221 ccgcgcggcg gcttcctgag cgg
//
LOCUS       DAOSPL010000095         5217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_15989_length_5217_cov_3.543588, whole genome shotgun sequence.
ACCESSION   DAOSPL010000095 DAOSPL010000000
VERSION     DAOSPL010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15989_length_5217_cov_3.543588"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PLQ54_02810"
     CDS             <1..325
                     /locus_tag="PLQ54_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HOS92218.1"
                     /translation="EAELHYAEEALPNMVAQSLARLRETRELRIRVSPGDLGRAEAIR
                     HQLMTVLEGVDGIDIIPDPRVDGGAVIETPMGTIDASLSGQLREIEQAMAEAATPDRG
                     ADEPA"
     gene            312..1673
                     /gene="fliI"
                     /locus_tag="PLQ54_02815"
     CDS             312..1673
                     /gene="fliI"
                     /locus_tag="PLQ54_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HOS92219.1"
                     /translation="MSQPDGAMASRLAPPPDLTPYFRAVERCDPKRLNGRTTRVVGLT
                     IESHGPACRIGEICELARRDGPPIVAEAVGFRDQFVLLMPLDDMEGIEPGSEVRATGR
                     CLRVPVGPGMLGRVLDGLGRPIDGGGDFEIETEYPVNARPPSPLLRSRITEPLEMGVR
                     AIDGPLTIGRGQRVGIFAGSGVGKSTLLGMIARNTEAAVSVVGLVGERGREVREFVER
                     DLGPQGLARSVVICATSDSPALVRLKAAFVATAVAEYFRDQGQHVLLMMDSITRVAMA
                     QREVGLAIGEPPATRGYTPSVFAMLPRLLERSGCSSRGTITALYTVLVEGDDMNEPIA
                     DQARSILDGHIVLSRRLAQRPHYPAIDVLQSVSRAMPDIVSPSHRRAADRLRDVLATY
                     QEAEDLINIGAYVPGSNPRIDYAQVMVQGANDFLKQDMLERTGYEEARLALESMFGQE
                     GVS"
     gene            1675..2163
                     /gene="fliJ"
                     /locus_tag="PLQ54_02820"
     CDS             1675..2163
                     /gene="fliJ"
                     /locus_tag="PLQ54_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOS92220.1"
                     /translation="MATRAFRFALQRVLDVRQVVERRVQTELASLETALERWRQERAS
                     LERRVEQAQDALRAAQQGEPDLVEQQLQRAHIRRLDQLIHAADAEIRLHMARIEAKRA
                     ELIQAATDRRVMERLRDRAYDAWVAEHLRREQIRLDEVGVQGFFRARNSPADPAPREG
                     TF"
     gene            2163..2753
                     /locus_tag="PLQ54_02825"
     CDS             2163..2753
                     /locus_tag="PLQ54_02825"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019626438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOS92221.1"
                     /translation="MDRPLDGLRAIEQSLSALRERLDQLHPARVLGDMAGVSPFAEAL
                     QRQMALQDEAAVDEATVESLGAGTAQWRGLIGKHAAQCGVDPTLVEAVMRVESGGAPH
                     SLSPAGAAGLMQLMPATARSLGVRDPFDPDESIQGGTRYLRQMLDRFGDLPRALAAYN
                     AGPAAVEQYGGVPPYPETQAYVRRVLDEARRLEPGG"
     gene            2757..3401
                     /locus_tag="PLQ54_02830"
     CDS             2757..3401
                     /locus_tag="PLQ54_02830"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92222.1"
                     /translation="MARAKGQAGKPAPGARGGSPDAAQADSGKPRRGCCLPRLVLALL
                     LTAMVCFVLHTLWIIDLPRVPHLRARPKRKPAAAATEVEKARAAEEAAGKPPGETAGP
                     EPAETGAVELGGLARAGAQPAPAGSSAPAAALKAQEDERKRAERRQQQKQAAIFAALS
                     PQKAAEALAAMPEDQQLQILQALEEKQAAKILEQMPVEKRVRVLTALGEPFAPL"
     gene            3577..>5217
                     /locus_tag="PLQ54_02835"
     CDS             3577..>5217
                     /locus_tag="PLQ54_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92223.1"
                     /translation="MQVMDVISALLGLSTSEGTVPGIAEGAAGFADVLQAALAGAGAL
                     GAGDATEVTAPSGSAREALAWLASALGLGSADTTSPTERGQPASVTWAALPEALRHRL
                     TQLDALAGMWLASVVARIASVEAEPPAGETAGSNPPPLPKAVDRVLKQLEEAGVVLPE
                     SLRALASMARGDEGSGTQGQSSVSAAQAVVDVTPVEARTRVFDEASAATVTIGERPET
                     VVVAVPELPRRGASPFGGTRADRWVPNAEPTRVAPAVARSEPNEGSTVRAAGVAPDDG
                     GLGPAVAAVTREASDAMQATTGSASVPVGGRLPALSSVDAAVQALLGALERTVEASDA
                     PQALQGGGAARVPTQALPEQFRVARVASELARQVGQVLAPTAGISVSPTQWLSALRTQ
                     FATGATLDGTAPAVDSPRPTGVVRAVPAEVVVAEADLPPTSAHGVSVWAEPTVSAVRP
                     AAGIVPSAGANPAWSSEGEEAGTVSQAPVAAVVASKTDPVPVTAGATASGWVARQEPS
                     LAPPATVRASDVPPVIRVGTVEFVTGAPVGPVPAPVAAE"
BASE COUNT          762 a         1758 c         1929 g          768 t
ORIGIN      
        1 cgaggccgag cttcactatg ccgaggaagc cttgcccaac atggtggccc agtcgctggc
       61 gcggctccgc gagactcgcg agctccgcat ccgcgtgagc cccggcgacc tgggccgggc
      121 cgaggcgatc cgccaccagc ttatgacggt gctcgagggt gtggacggca tcgacatcat
      181 cccggacccc cgggtcgacg gaggcgctgt catcgagacg cccatgggca ccatcgacgc
      241 ctcgctgtcc gggcaacttc gcgagatcga acaggcgatg gctgaggcag ccacaccgga
      301 tcggggggcc gatgagccag cctgatggcg ccatggcatc gcgactcgcg cctccgccgg
      361 atctgacgcc ctacttccgc gcggtcgagc ggtgtgaccc gaagcggctg aacggccgca
      421 cgacccgagt cgtcggactc accatcgagt cccacggccc cgcctgtcgg atcggcgaga
      481 tctgtgagct cgcgcggcgg gacggtcccc cgatcgtcgc cgaggccgtg gggttccgtg
      541 atcagttcgt gctgctgatg ccgctggacg acatggaggg catcgagcct ggcagcgagg
      601 tccgggcaac cggccgttgc ctgcgcgtgc cggtgggtcc gggaatgctg ggccgagtgc
      661 tggatggcct cggacggccc atcgacgggg ggggcgactt cgagatcgag accgagtacc
      721 cggtcaatgc ccggcctcca tcgccgctcc ttcgctctcg gatcaccgaa cccctcgaga
      781 tgggggtccg agccatcgac ggcccgctca ccatcggccg ggggcagcgt gtgggcatct
      841 tcgccggcag cggcgtcggc aagagcacgc ttctgggcat gatcgcccgc aataccgagg
      901 ccgcggtgag cgtggtcggg cttgtgggcg agcgcggacg cgaggtccgg gagttcgtcg
      961 agcgggatct cggaccccag ggcctggccc ggtcggtggt gatctgtgcc acgtccgact
     1021 ccccggcgct cgtccgcctc aaggcggcgt tcgtcgccac ggcggtggcc gagtacttcc
     1081 gcgatcaggg ccagcatgtg cttctcatga tggactcgat cacgcgggtg gccatggccc
     1141 agcgtgaggt gggcttggcc atcggcgagc caccggccac ccggggctac acaccatcgg
     1201 tcttcgccat gctgccgaga ctgctcgagc gctcgggctg ctcgagccgc ggcaccatca
     1261 ccgccctcta tacggtcctg gtcgagggcg atgacatgaa cgagccgatt gccgaccagg
     1321 cgcgcagcat tctggacggc cacattgttc tgtcccgccg cctggctcag cgtccgcact
     1381 atccggccat cgatgtgctg cagtcggtct ctcgggccat gcccgacatc gtgtccccat
     1441 cgcatcggcg ggccgcggac cgccttcgcg atgtactcgc cacctatcag gaggccgagg
     1501 acctgatcaa catcggcgcc tatgtcccgg gctccaaccc aaggattgac tacgctcagg
     1561 tcatggtcca gggcgcgaac gatttcctga agcaggacat gctggagcgc accggctatg
     1621 aagaggcccg cctggcactc gagtccatgt tcggccaaga gggcgtgagc tgagatggcc
     1681 acccgcgcct tccggttcgc gcttcagcgg gtgctggacg tccgccaggt ggtcgagcgg
     1741 cgggttcaga ccgagctggc cagcctcgag acggctctcg agcgctggcg gcaggagcgt
     1801 gcgtcgctcg agcgacgtgt ggagcaggcc caagacgctc tccgtgccgc ccagcaaggc
     1861 gaacccgacc tggtcgagca gcagctgcag cgcgcccata tccggcgtct ggatcagctg
     1921 atccacgccg ccgacgccga gattcggctg cacatggccc gcatcgaggc caagcgggcc
     1981 gagctcatcc aggccgcgac cgaccggcgg gtgatggagc ggctgcgtga cagggcctat
     2041 gacgcgtggg tggccgagca tctgcggcgg gagcagatcc gtctcgatga ggtgggcgtg
     2101 cagggcttct tccgtgcgcg gaactccccc gcagatcccg cgccacggga ggggaccttc
     2161 tgatggacag accactggac ggtctgcgcg ccatcgagca gagcctgagc gcgttgcggg
     2221 agcggctcga ccagctccac cccgcgcgcg tcctgggcga catggcggga gtgtccccct
     2281 tcgccgaggc tctgcagcgt cagatggccc ttcaggatga ggcggcggtc gacgaagcca
     2341 cggtcgagtc attgggagct ggaactgccc agtggcgtgg gctcatcggc aagcatgccg
     2401 cccagtgcgg ggtcgatccc acgctggttg aggcggtcat gcgagtcgag tccggcggag
     2461 cgccgcacag cctatcgccc gccggcgccg ccggcctcat gcagctcatg cccgccaccg
     2521 cccggtcgtt gggtgtgcgg gacccattcg accccgacga gagcattcag ggcggcacgc
     2581 gctacttgcg ccagatgctc gaccgcttcg gcgacctgcc gcgtgcgctg gcggcctata
     2641 acgccggacc ggccgcggtc gagcagtatg ggggcgttcc cccctaccca gagacccagg
     2701 cctatgtgag acgcgtgctc gatgaagcac ggcgtctgga gccggggggc taagccgtgg
     2761 cacgcgccaa gggacaggca ggcaagccgg ccccgggtgc ccgaggaggg tcgcctgatg
     2821 ccgcgcaggc ggattccggc aagccgcggc ggggctgctg cctcccgcgc cttgtcctcg
     2881 cgctcctgct gaccgccatg gtctgcttcg tcctccacac gctctggatc atcgacctac
     2941 cgcgagtgcc gcatcttcgg gcccggccca agcgaaagcc cgcggcagcg gccaccgagg
     3001 tcgagaaggc tcgagcggcc gaggaggcgg cgggaaagcc gccgggcgag acggcgggcc
     3061 ccgagccggc cgagacgggc gctgtggagc tcggcggact cgcccgtgcc ggggcccagc
     3121 cggcgccggc cggatcatcg gcgccggcgg cggcgctcaa agcacaggaa gacgagcgca
     3181 agagagccga gcgtaggcag caacagaaac aggcggccat cttcgcggcc ctctcgccgc
     3241 agaaggccgc cgaggccctg gcggcgatgc ctgaggacca gcagctgcag atcctgcagg
     3301 ccctggagga gaagcaggcg gccaagatcc tcgagcagat gccggtcgag aagcgggtgc
     3361 gggtcctgac ggccctcgga gagccgttcg ccccgctcta ggcagtacct gccggcgagc
     3421 atgctcgctg gcagagcgtt ctggcccgtg gagacgggtc ggaccaggtt gcggtcggtg
     3481 gcgaggtgcc ctcgcgcccg ccgctccgcc ggcaggcata ggtctgtcgg gcacgacaac
     3541 ggggctctgc cccagaggaa gggaggtgag atcatcttgc aggttatgga tgtaataagt
     3601 gccctcctgg gactatcgac ctcggagggc acggttccag ggattgcgga aggcgctgcc
     3661 ggcttcgccg atgtgctcca ggcggcgcta gccggggcgg gtgcgctcgg tgccggggac
     3721 gcgacggagg tgactgctcc gtccggaagt gcccgggagg cactggcgtg gctggcctcg
     3781 gccctgggct tgggttcggc ggacaccacg tcacccaccg agcggggaca gcccgcttcc
     3841 gtgacgtggg ccgctctgcc cgaagcccta cggcatcgtc tgacgcagct cgacgcactg
     3901 gccggcatgt ggctggccag tgtggtggct cgcatcgcct cggtggaggc cgaacccccg
     3961 gctggggaga cggccggatc gaacccaccg ccgttgccca aggccgtcga tcgggtgctg
     4021 aagcagctcg aggaggctgg tgttgtgctg cccgagtctc tccgcgccct ggccagtatg
     4081 gccaggggtg atgagggctc aggaacgcaa ggccagtcgt cggtctccgc agcgcaggcg
     4141 gtggtggacg tcacgccggt cgaggccagg acgcgagtct tcgacgaggc cagcgcggcg
     4201 acggtcacga tcggcgagcg gccggagacc gtcgtcgttg cggtccccga gctgccgcgg
     4261 cgtggcgcga gtccgttcgg ggggactcgt gccgatcggt gggtgccgaa cgcggagccc
     4321 acccgggtcg cgcctgcggt agcccggtct gagccgaacg aagggtccac cgtgcgggcg
     4381 gcgggcgtgg cgccggatga tggtggtttg ggtcccgcag tcgcggccgt cacgcgcgag
     4441 gcgagtgatg ccatgcaggc aacgaccggc tccgcgagcg tgccggtcgg cggccgcctg
     4501 ccggcgttgt cgtcggtgga tgccgcggtg caggctctcc tgggtgcgct cgagaggacc
     4561 gtcgaggcat cggacgcgcc gcaagcgcta cagggcggcg gcgctgcccg tgtgccgact
     4621 caggcgcttc ccgagcagtt ccgcgtcgct cgagtggcat ctgaactcgc gcgacaggtg
     4681 ggccaagtgc tggctcccac ggccgggata tccgtgtcgc cgacccagtg gctgagcgcc
     4741 ctgcgcaccc agttcgcgac cggcgcgacg ctggatggaa cggcgccggc agtcgactcg
     4801 ccgcgaccca caggtgtggt tcgtgcggtg ccggcggagg tggtggtggc cgaagccgat
     4861 ctgccgccga ccagcgccca cggtgtttcc gtctgggccg agccaacggt ctcggccgtg
     4921 cgaccggcgg ccgggatcgt gccttccgcc ggagcgaatc cggcgtggtc gagcgaggga
     4981 gaggaggccg gcacagtctc ccaggcgccg gtggcggccg tggtggccag caagaccgat
     5041 cccgtgcccg tcacggccgg ggctacggcc tcggggtggg tggcacggca agaaccatcg
     5101 ctcgcacccc cggcaaccgt gcgggcttcc gatgtgccgc cggtgatccg ggtcggcacg
     5161 gtggagttcg ttaccggagc gccggtgggc ccggttcctg caccggtggc agccgag
//
LOCUS       DAOSPL010000096         5184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_16144_length_5184_cov_3.226360, whole genome shotgun sequence.
ACCESSION   DAOSPL010000096 DAOSPL010000000
VERSION     DAOSPL010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16144_length_5184_cov_3.226360"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            350..1120
                     /locus_tag="PLQ54_02840"
     CDS             350..1120
                     /locus_tag="PLQ54_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOS92224.1"
                     /translation="MADLKLSACIEMIFSNVPNFVDRIDLAAECGLPAFEFWGWHGKD
                     LPAIRQRADAKGLTIAAMCCDASGALVDPSNTAAWIAAASESIEKGKEFGLPTMIVTT
                     GNEMPIPREKQHAAIVAGLKGIAPKAEAAGITIVLEPLNIRTDHMGYYLSTSDEGFEI
                     INEVGSPRVKLLFDIYHQQITEGDLTVRITRNIDLIGHFHCADVPGRHEPGTGEINYV
                     NVFRAIAKTDYAGYIGLEFSPTADHREAMASTRQIAGV"
     gene            1149..2048
                     /locus_tag="PLQ54_02845"
     CDS             1149..2048
                     /locus_tag="PLQ54_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS92225.1"
                     /translation="MTRIGMVTGIALFHGPAFGALLHHRRDRDAWAAAGFWEPGVDPI
                     EGVEITGVYDQNREAAEKLVALLPGAWVADRPDDLLGHVDGILIADDGTQQHQKYAEP
                     FLRAGIPTFIDKPLSRDWREAASILALAEELGTPCMSSSALRYAREVDEAWEQIEAVS
                     PIKLALAVGPNELIYYGIHPAELMHRVLGPGVLSVHNVGRERLNVVRLAYADGRMGVL
                     CVSEEIGYVFELRLYGEKGAVTVAVNDAAAFYRNLLAAVIGMVRTGIPPIPYSSTLEV
                     IKILDGAKRSLATGLPVELPLMD"
     gene            complement(2056..3297)
                     /locus_tag="PLQ54_02850"
     CDS             complement(2056..3297)
                     /locus_tag="PLQ54_02850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HOS92226.1"
                     /translation="MLSGLRHISSILVKPAGPDCNLACRYCFYRSAAALYPDRPVHRM
                     SDEVLESVICQYMAIAGPLASFGWQGGEPTLMGLPFFERVVQLQHRYGLGGQKVSNGL
                     QTNGVLIDDDWARFLAQWKFLVGLSLDGPAEIHDHYRRDHGGRATHARVLEALHHLQH
                     ARVDTNVLCMVTDASVTRARELFDYFYGLGIRWMQFIPCVETHPDGGWESYSLDVAEY
                     GQFLCALFDLWYGGMNLKRTYIRDFEELLINVTTGRPPSCVPSDHCGDYVVVEHNGDV
                     YACDFHVTPEWRLGNLMETPLLELVNSPTMERFAAAKRQWDRSCTTCPWLNLCHGGCP
                     KYRINANGAPARKTCFCAANLELFRHAGDTLRELADRYGPPSVLHRVPEVHDASSGVR
                     GNAPCPCGSGRKYKHCCMKRG"
     gene            complement(3418..4710)
                     /locus_tag="PLQ54_02855"
     CDS             complement(3418..4710)
                     /locus_tag="PLQ54_02855"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOS92227.1"
                     /translation="MERDDARRITELALSAATVECTEVYVGTTAAATTRFSDNSITQN
                     LSQTDRYIAIRSAIGNKVGVATANDLSDESIRQVVRRAEDIARAAEPDTEYVPPVGPQ
                     DYPDIPAFAEATAVCDPEQRADAVAAVVATATAGGMRSSGSISTSADVSAIANSLGLF
                     GFHRSSRFALNVTALTEDSSGWAESSGVDVSRLDATDVARRACEKAAMSRNPIDVDPK
                     PYVTILEPPALLEFLEFMMWDLDAKEADEGRSGFAGKEGTVIGQPSVTIRSDPSHPEC
                     PGSPWTNGGVITPKVTWVEQGTLKTLSYSRFWAQKTGKPFTGSPSNILMSGEDHDIED
                     LIASVDEGLLVTRFWYIRTVDPMALLHTGMTRDGLFWIEKGRIKCGVRNLRFNESQLR
                     MLSQVRMMGRATPTAMYSRGVVPPVLLDSFQFTSGTKF"
     gene            4950..>5184
                     /locus_tag="PLQ54_02860"
     CDS             4950..>5184
                     /locus_tag="PLQ54_02860"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOS92228.1"
                     /translation="MDVLSILGSPRRAGNTARVLDWVEEVFHEAGSAVERISIGDRDI
                     RPCTGCFACKLQPGSMCIQRDDANELFRQIRDAD"
BASE COUNT          875 a         1708 c         1686 g          915 t
ORIGIN      
        1 aacgacgcaa gtccgatcac tcccctcgcg ccccgggccc gcgtcccttt tgggggcccg
       61 gccggcggca gataaggggg gtgccacacg ggccggcctg actggccggc cttccgatcg
      121 acggtctccg gtaccaatcg ccggagacga gacaggtacc tctggctccg agcgcgctgg
      181 tttgtctgtt cggcgtgtcg ccgggcgaat cattgcgttc atgaaagatt tccgtgcctg
      241 gatcgcgccg ggcgccggat tccccgagtc ctggtgtgcg aaggtcagtc ggacggagcg
      301 gccgaaaggc tcacagacgc ggcgcgatcc aacacccgag gagaccccaa tggctgatct
      361 caagctctct gcctgcatcg agatgatctt cagcaacgtg ccgaacttcg tcgaccgcat
      421 cgacctggcc gccgagtgcg gtcttcccgc attcgagttc tggggctggc acggcaagga
      481 cctgccggcg atccgccagc gagctgacgc caagggcctc accatcgcgg ccatgtgctg
      541 cgacgcttcc ggtgcgctcg tggatcccag caacactgcg gcatggatcg ctgccgccag
      601 cgaatccatc gagaagggca aggagttcgg gcttccgacc atgatcgtca ccacgggcaa
      661 cgagatgccc attccccgcg agaagcagca cgcggccatc gtggccgggc tcaagggcat
      721 cgccccgaag gctgaggccg ccggcatcac catcgtgctc gaaccactca acatccggac
      781 tgaccatatg gggtactacc tgtcgacgtc cgatgagggc ttcgagatca tcaacgaggt
      841 cggtagccca cgggtcaagc tcctcttcga tatctaccac cagcagatca ccgagggcga
      901 ccttacggtg cgtatcacga ggaatatcga tctcatcgga cacttccatt gtgcggacgt
      961 gcctggccgg cacgagccag gcacgggtga gatcaactac gtgaatgtct tcagggccat
     1021 cgccaagacg gactacgcgg gctacatcgg cctcgagttc agccccacgg cggatcaccg
     1081 cgaggccatg gccagcacgc gccagatcgc cggcgtctga ccgcgcgcgt gagagcgagg
     1141 gaggcgctgt gacccgcatc ggtatggtga cgggcatcgc cttgttccac gggcccgcct
     1201 tcggcgcgct gctgcatcac cggcgcgacc gcgacgcctg ggcggccgcc ggcttctggg
     1261 agccgggcgt ggaccccatc gagggagtcg agatcactgg ggtctacgac cagaaccgcg
     1321 aggccgccga gaagctcgtc gcccttctgc ctggcgcctg ggtggctgac cggcccgacg
     1381 acctgctcgg gcatgtcgat ggcatcctca tcgccgatga cggcacccag cagcaccaga
     1441 agtacgccga gccgttcctc cgggccggta tccctacgtt catcgataag ccgctgtcac
     1501 gcgactggcg ggaggccgcc tcgatcctgg cgctggccga ggagctcggg acaccgtgca
     1561 tgtcctcgtc ggcccttcgg tacgcccgcg aagtcgatga ggcgtgggag cagatcgaag
     1621 ccgtctcgcc catcaagctc gccctcgccg tgggacccaa tgagctgatc tactacggca
     1681 tccacccggc tgagctgatg caccgcgttc tcggcccggg tgtgctctcg gtgcacaacg
     1741 tcggtcgcga gcggctgaac gtggtgaggc tggcctatgc cgacgggcgc atgggcgtgc
     1801 tgtgtgtgtc ggaggagatt ggctacgtct tcgagctacg cctctatggc gagaagggcg
     1861 ccgtcacggt cgcggtcaac gatgccgcgg cattctacag gaacttgctc gctgcggtga
     1921 tcgggatggt tcgcaccggc ataccgccca ttccgtacag ctcgacgctc gaggtcatca
     1981 agatcctgga cggtgcgaag cgctccctgg cgaccggtct gccggtcgag ctgccgctca
     2041 tggactgacc ggggcctacc cccgcttcat gcagcaatgc ttgtacttcc gcccactccc
     2101 gcaggggcag ggagcgttgc ctctgacgcc tgatgaggca tcgtgcacct cgggtacacg
     2161 gtgcagaaca ctcggcgggc cgtaccgatc ggcgagttcg cgtagcgtgt cgcccgcgtg
     2221 cctgaagagc tcgaggttgg cggcgcagaa gcacgtcttt cgcgccggcg cgccgttggc
     2281 attgatgcgg tacttggggc accctccgtg gcagaggttc agccacgggc atgtggtgca
     2341 tgaccgatcc cactggcgct tggcggccgc gaatcgttcc attgtcgggg agttcacgag
     2401 ttccagcagg ggagtctcca tcagattccc cagccgccac tccggtgtca cgtggaagtc
     2461 acatgcatat acatcaccat tgtgctccac cacgacatag tcgccacagt gatcgctcgg
     2521 gacgcaactg ggcggacggc cggtggtcac gttgatcagg agctcctcga agtcgcggat
     2581 gtacgtgcgc ttcaggttca tgccaccgta ccagagatca aacagggcgc aaaggaattg
     2641 tccatactcg gccacatcga gcgagtagga ctcccaaccg ccgtcgggat gggtctcaac
     2701 acatgggatg aactgcatcc accgaatgcc caggccgtag aagtagtcga aaagctcccg
     2761 cgcccgagtg acgctcgcgt cggtgaccat gcacagcacg ttcgtgtcca ctctcgcatg
     2821 ctggaggtgg tggagggcct ccagcacccg cgcgtgcgtc gctcgtcccc cgtggtctcg
     2881 ccgatagtgg tcgtggatct ctgccgggcc gtcaaggctc aagcccacca ggaacttcca
     2941 ctgcgccagg aaccgggccc agtcgtcatc gatgagcacc ccgttggtct gcagaccgtt
     3001 ggataccttc tgtccgccca ggccgtagcg gtgctgcagc tgcacgacgc gctcgaaaaa
     3061 ggggaggccc atgagtgtgg gttcgccgcc ctgccagcca aaggaagcca agggccccgc
     3121 gatggccatg tactggcaga tgaccgactc cagtacctcg tcactcatcc ggtggaccgg
     3181 ccggtccgga tacagcgcgg cagcgctgcg atagaagcag tagcggcagg ccaggttgca
     3241 gtccggtccg gccggcttca ccagaatcga cgagatgtgg cgaaggccac taagcatgag
     3301 tctgggcggc gagcatactg acggcagggc agatgccgtg agaggcgtgc gaaggggcca
     3361 tagggctgat tgagtggcac agcctccggt cggtgctccc tctcccctcc taccccctca
     3421 gaacttcgtt ccactcgtga actgaaagct gtcaagcagc acggggggca cgacgccccg
     3481 gctgtacatg gcggtgggtg tggcgcgacc catcatccgc acctggctca acatgcggag
     3541 ctggctctcg ttgaagcgca ggttgcgcac tccgcacttg atgcgaccct tctcgatcca
     3601 gaacagcccg tcccgtgtca tccccgtgtg gaggagtgcc atgggatcga ccgtccggat
     3661 gtaccagaat cgggtcacca gcaggccctc atcgacggac gcgatcaggt cctcgatgtc
     3721 gtgatcctct ccggacatga gaatgttgga gggtgacccg gtgaatggct tccccgtctt
     3781 ctgggcccag aagcgggagt aggagagcgt cttcagtgtg ccctgttcga cccaggtcac
     3841 cttgggcgtg atgacgccgc cgttggtcca gggcgagccg gggcactcgg ggtgtgatgg
     3901 gtctgagcga atggtgaccg agggctgtcc gatcactgtg ccttctttgc ccgcgaaacc
     3961 tgagcggccc tcgtcggcct ccttggcatc cagatcccac atcatgaact cgaggaactc
     4021 cagcagcgcc ggcggctcga ggatggtcac atagggcttc gggtccacgt cgatggggtt
     4081 gcgcgacatg gccgccttct cgcacgcgcg ccgggccacg tcggtggcgt ccagccggct
     4141 gacatcgacg ccggacgact ccgcccagcc ggagctgtcc tccgtcaagg cggtcacgtt
     4201 gagcgcgaag cgactcgacc ggtggaaccc aaacagtccc aacgagttgg caatcgctga
     4261 cacgtcggcg ctggtgctga tgctgcccga cgaacgcatc ccgccggctg tcgccgtcgc
     4321 cacgacggcg gccaccgcgt cggcgcgctg ttcgggatca cagaccgccg tggcttcggc
     4381 gaacgccggt atgtccgggt agtcctgcgg gccgaccgga ggcacatact ccgtgtccgg
     4441 ctccgcggcg cgggcgatgt cctccgcacg ccgcaccacc tggcggatgc tctcgtcact
     4501 caggtcgttg gccgttgcca cgccgacctt gttcccgatg gcggaacgga tggcgatgta
     4561 ccggtcggtc tgcgataggt tctgagtgat cgaattgtcg ctgaaacgcg tcgtggccgc
     4621 tgctgtggtg cccacatata cctccgtgca ctctacagtg gcggccgaca gggccagctc
     4681 ggtgatgcgg cgtgcatcgt cgcgttccac agtcgtctcc tccgtgcgca agtcgggcgc
     4741 ggccgggatt gtgtctcccg ggggtttcgg ccaacgggtg ccggaggcct ctgccctctg
     4801 gcgaggccac gggacgccga gttctcctcg gcccaacatc tgaacccggc cgtgactccc
     4861 tacgttaccc acttggcagc cagggccggg ggcggtcagt cggggtcacc tcgactggtc
     4921 gccctctctt ttcatgtggg ggtgccacca tggacgttct gtccatcctc ggcagcccgc
     4981 ggcgcgcggg gaacacggct cgggttctcg actgggtcga ggaggtcttc cacgaggccg
     5041 gctccgctgt cgaacgaatc agcatcggcg atcgcgacat ccgtccgtgc accggatgct
     5101 tcgcctgcaa gctgcagccc ggctcgatgt gcatccagcg cgacgacgcc aacgagctgt
     5161 tccgccagat ccgcgacgcc gacg
//
LOCUS       DAOSPL010000097         5165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_16210_length_5165_cov_3.248141, whole genome shotgun sequence.
ACCESSION   DAOSPL010000097 DAOSPL010000000
VERSION     DAOSPL010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16210_length_5165_cov_3.248141"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /gene="valS"
                     /locus_tag="PLQ54_02865"
     CDS             complement(<1..1269)
                     /gene="valS"
                     /locus_tag="PLQ54_02865"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOS92229.1"
                     /translation="MGLDQLKDAYDPRETEARWYERWLSDGVFTPDPGSGKPPYCIVI
                     PPPNVTGALHMGHALQDTIMDLLTRYHRMAGYESLWLPGTDHAGIATQAVVERHLARK
                     GISRHDLGRERFVDEVWAWKHQYHARITEQLKRLGCGVDWTRERFTLDDGLSRAVRLV
                     FCQLYDDDLIYRGPYIINWCPHDRTTISDEEVVADNEVDGHLWHIEYPLADGSGGIVV
                     ATTRPETMLGDTAVAVNPGDPRHADKIGKTVILPLMGREIPIIGDDRVELGFGAGALK
                     VTPAHDKTDYEIGRDHNLPQISVIGRDGRMTEAAGAYAGLDRFDARQRVLADLEAQGL
                     LRHTEDYRHTVPICSRCDTVIEPLISTEWFVRMRPLADRAIEAQRAGKMRYVPARWES
                     MYLNWLENIRDWPISRQLWWGHRIPAWHCDD"
     gene            complement(1489..4008)
                     /gene="lon"
                     /locus_tag="PLQ54_02870"
     CDS             complement(1489..4008)
                     /gene="lon"
                     /locus_tag="PLQ54_02870"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOS92230.1"
                     /translation="MRKPRDLDDILPPEGLTDRGGLEPPALTPPTTAPRGGPDRDEDE
                     VLPPLPVLPLRDQVFFPAMVMPLFFSRPKSIHAIEDAMLHGQRLVLVAQRDPNLEEYE
                     PKDIFDVGVLAQSLQVLKLPDGSLRMVAEGQARVRIQAFTHLDPFMKAFVEPIEDIPY
                     EGSEAEALMRTIVAQFERAGELSRNIAPEAVTAASRITDAGRLSDLVASNLNATIEEK
                     QDLLATADVHERLQRLHLHLAHEIEILELERKIHSRVREEIQASQREMYLREQLRVIR
                     DELREGSGEPGEIDEYERRITEAQMPEEAEAHARRELGRLERTPMMSPEGSVIRTYLD
                     ALCDLPWQATTDDSTSISVAERVLDEDHYGLRRVKERVLEFLAAKQLAGKDLQSPILC
                     FVGPPGVGKTSIGKSIARALGRKFIRVSLGGVRDEAEIRGHRRTYIGAMPGRIMQAIR
                     QVGVRNPVFMMDEIDKLGMDFRGDPSAALLEVLDPEQNNSFSDHYLEVKFDLSEVMFI
                     TTANLLDPVPPALRDRMEVIEFDGYTEFEKLEIAHRYLAPKQLKANGLTAGQLTFPKA
                     ALRDIIRSYTREAGVRSLEREIARVCRKVCRKVASAQGEPITRVRFTRADVPVYLGPA
                     KYRFGLAEHEDQVGVAAGLAWTSAGGQVIMVEVCLVPGRGQVLLTGSLGETMKESAET
                     AMTFARSWNAAVGGQERWFRNSDVHVHLPEGAVPKDGPSAGITIATALCSALSRRPVK
                     CSVSMTGEVTLRGRILPVGGVKEKVLAAHRGGLRTVLLPAENEKDIEELPAEVLRDLE
                     IVFVREMSEVLNRALAKAPIEQRAGHAKPGRAGASAAGAQS"
     gene            complement(4095..>5165)
                     /gene="clpX"
                     /locus_tag="PLQ54_02875"
     CDS             complement(4095..>5165)
                     /gene="clpX"
                     /locus_tag="PLQ54_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOS92231.1"
                     /translation="PQDIKRVLDQYVVGQELAKKTLSVAVYNHCKRIAMLDADPDVEL
                     GKSNILLIGPTGCGKTLLAQTLARILDVPFAIADSTALTEAGYVGEDVENILLKLLIA
                     ADHDIEACQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKILEGTVANVPPQG
                     GRKHPQQEYVQIDTSGILFICGGTFSGLEDIIRGRINKQSMGLGAKLTRREEQGLAEL
                     LSNVEPDDLRKYGLIPEFIGRLPVVACLDPLGEEALVQILTEPRNALIKQYSRLLAHD
                     GVELSVDEEAQRAIAREAKRRGTGARALRTIIEEIMLDVMYDIPSQKNVRRVVVNTDC
                     VLAGKPPVVEYNGGRDAEQKTA"
BASE COUNT          825 a         1671 c         1718 g          951 t
ORIGIN      
        1 gtcgtcgcag tgccatgccg ggatgcggtg cccccaccag agctggcgcg agatgggcca
       61 gtcgcgaatg ttctcgagcc agttgaggta catcgactcc cagcgggcgg gcacgtatcg
      121 catcttcccc gccctctggg cctcgatggc gcggtcggcc agggggcgca tccggacgaa
      181 ccactccgtc gagatcaggg gctcgatgac cgtatcgcaa cgcgagcaga tcggcacggt
      241 gtggcggtag tcttcggtgt gtcggagcag gccctgggcc tcgagatccg ccaggacgcg
      301 ttggcgcgca tcgaagcgat ccagacccgc gtaagccccg gcagcctccg tcatgcgacc
      361 atcgcgtccg atgacgctga tctgcggcag gttgtggtcc cggccgatct catagtccgt
      421 cttgtcatgg gccggcgtga ccttcagcgc gccggcaccg aaacccagct cgacccgatc
      481 atcgccgatg atcgggatct cgcgccccat caggggcaga atgacggtct tgccgatctt
      541 gtccgcgtgg cgcgggtcgc cggggttcac ggctaccgcg gtgtcgccca gcatggtctc
      601 cgggcgcgtc gtggccacca cgatgccccc cgagccgtcg gccagcgggt actcgatgtg
      661 ccacaggtga ccgtcgacct cgttgtcggc gacaacttcc tcgtcggaaa tggtcgtccg
      721 gtcatgaggg caccagttga tgatgtaggg tccgcggtag atcagatcgt cgtcatagag
      781 ctggcagaag actaggcgca cggcccggga gaggccgtca tcgagcgtga agcgctcgcg
      841 cgtccagtcc acgccgcagc ccaggcgctt gagctgttcg gtgatgcgcg cgtggtactg
      901 atgcttccac gcccatacct cgtcgacgaa ccgctcgcgg cccaaatcgt gccggctgat
      961 gcccttccgg gccaggtgcc gctcaaccac ggcctgggtg gcaataccgg catggtcggt
     1021 gccgggaagc catagtgact catagccggc catgcggtgg tagcgagtca gcaggtccat
     1081 gatggtgtcc tgcagggcat ggcccatatg cagagcgccg gtgacgttgg gtggcgggat
     1141 gacgatgcag tagggcggct tgccacttcc agggtctggg gtgaacactc catcgctcag
     1201 ccatcgctca taccagcggg cttcggtctc gcgggggtcg taggcgtctt tgagctgatc
     1261 taggcccatg gggaccacac tcccgaaggg ttgactcagg tgtggggcag cgacaagcgc
     1321 cccgttggag gcaggtggac tgggaacctg cgggagggtg ccggaggtca ggcacgcctg
     1381 aggcgtatgc gggtgcgggc ggcacgtcgg gacgagccgc ccccggctac agcgacatcg
     1441 tgtcgcctct gtggtcccgg tgtggcacta aggtacatgg gcgttcccct acgactgagc
     1501 cccggcggcc gaagcgccag cacgccctgg cttcgcatgg cccgcccgct gctcaatcgg
     1561 cgctttggcc agggcccggt tcagcacctc gctcatctcg cgcacgaaca cgatctcgag
     1621 atcgcggagc acctcggcgg gcagttcctc gatgtccttc tcattctcag cgggcagcag
     1681 aacggtgcgc aacccgccgc ggtgggcagc caacaccttc tctttgacgc cacccacggg
     1741 cagaatacgc ccacgaagtg tgacttcacc cgtcatcgac accgagcact tcaccggcct
     1801 gcgtgagagt gcagaacata gtgccgtggc gatggtgatc cccgccgagg gtccatcctt
     1861 gggtacggct ccttcgggaa ggtgcacgtg cacgtcagag ttgcggaacc agcgctcctg
     1921 gcctcccact gcggcgttcc atgaccgggc aaaggtcatg gccgtctcgg ccgactcctt
     1981 catggtctcg cccaagctgc cggtgagcag aacctgccct cgacccggca cgaggcagac
     2041 ctcgaccatg ataacctggc caccggccga ggtccacgcg agtccggccg cgacgccgac
     2101 ctggtcctcg tgttccgcca ggccgaaccg gtacttggcg ggtcccaggt acacgggaac
     2161 atcggcccgg gtgaaccgaa cgcgcgtgat gggctcgccc tgggcgctcg ccactttccg
     2221 gcagactttg cggcagaccc gggcgatctc gcgttcgagg cttcggacac cggcctcacg
     2281 cgtgtagctg cggatgatgt cgcgtagggc ggccttgggg aatgtgagtt ggccggcagt
     2341 gaggccgttg gccttcagtt gcttcggcgc cagatagcgg tgcgcgatct cgagcttctc
     2401 gaactcggtg tagccgtcga actcgatgac ctccatgcgg tcgcgcaggg cggggggcac
     2461 cgggtcgagc agattggcgg tggtaatgaa cattacttcg ctcaggtcaa acttcacttc
     2521 gaggtagtga tccgaaaatg agttgttctg ctcggggtcg agcacctcga gcaatgccgc
     2581 tgagggatcc ccccggaagt ccatcccgag tttgtcgatc tcgtccatca tgaatacggg
     2641 gttgcggaca cccacctggc ggatcgcctg catgatgcga ccgggcatcg cgccgatgta
     2701 cgtccgccgg tggccgcgaa tctcggcctc gtcgcgcaca ccgcccagcg atacgcggat
     2761 gaacttgcgc ccaagggcac gcgcgatcga cttgccgatg gaggtcttcc cgacacccgg
     2821 aggaccgacg aagcagagga tgggcgactg caggtccttg cccgcgagct gcttggcggc
     2881 gagaaactcg agcacccgct ccttgacgcg gcgcaggccg tagtgatctt cgtccagcac
     2941 tcgttcggcc acagaaatgc tcgtgctgtc gtccgtggtc gcctgccagg gcaggtcgca
     3001 cagagcatcg aggtaggtgc ggatcacgga cccctcgggt gacatcatgg gggtgcgctc
     3061 gagtcgcccc agttcacgcc gcgcgtgtgc ctcggcctct tcaggcatct gagcctcagt
     3121 gatgcgccgc tcgtactcat cgatctcacc gggctcgcca ctcccctcac ggagttcgtc
     3181 tcggatcacc cggagctgct cgcggaggta catctcgcgc tgcgacgcct ggatctcctc
     3241 ccgcacgcgc gagtggatct ttcgttccag ttcgaggatc tcgatctcgt gggccaagtg
     3301 caggtgcaaa cgctggagcc gctcgtgcac gtccgcggtg gcgagtaggt cctgcttctc
     3361 ctcaatggtc gcgttgagat tggaggcgac caggtccgac aggcggccgg catcggtgat
     3421 cctcgacgcg gcggtcaccg cctccggcgc aatgttacgc gacagctcgc ccgcccgttc
     3481 gaactgagcc acgatggtgc gcatgagggc ctcggcctca gatccctcgt aggggatgtc
     3541 ctcgatgggc tccacgaagg ccttcatgaa tgggtccaag tgggtaaacg cttggatgcg
     3601 gacccgtgcc tgaccctcgg cgaccatccg caggctgccg tcgggcagct tcagcacctg
     3661 caggctctgc gccagcacgc cgacatcgaa gatgtccttg ggctcatact cctcgaggtt
     3721 ggggtcgcgc tgggctacca ggaccagcct ctgaccatgg agcatggcgt cctcgatggc
     3781 gtggatcgac ttggggcgag agaagaacag cggcatgacc atggcgggga agaagacctg
     3841 gtcgcgcaag ggcagcacgg gcagcggagg caggacctcg tcctcgtcgc ggtcgggacc
     3901 gccgcgcggc gctgtcgtgg gcggagtgag tgccggaggt tcgagacctc ccctgtccgt
     3961 cagcccctcg ggcggcagaa tgtcatccag gtctctgggt tttctcacag gcacggctcc
     4021 aagctgatgc ccggcgggct gcagggcccg accgggcctc cggctcttcg gtgtccgggg
     4081 cgtgttgggg cgggttaggc cgtcttctgc tcggcatccc ggcccccgtt gtactcgact
     4141 accggaggct tcccggccag cacgcagtcg gtgtttacga ccactcgccg gacgttcttc
     4201 tgcgagggga tgtcgtacat gacgtcgagc atgatctcct caatgatggt tcgtagagcc
     4261 cgggccccag taccgcggcg tttggcctca cgggcaatgg ctctctgggc ctcttcatcc
     4321 acagagagct ccacgccgtc gtgtgccagc aggcgggagt actgcttgat cagagcattc
     4381 cgcggctcgg tcaggatctg gacgagtgcc tcctcgccca gggggtccaa acacgcgaca
     4441 acgggcaggc gtccgatgaa ctcggggatc agcccgtact tgcgcaggtc atcgggctcc
     4501 acgttggaca gcagctcagc cagcccctgc tcctcgcgcc gggtcagctt ggcccccagc
     4561 cccatggact gcttgttgat ccgtccgcgt atgatgtcct cgagcccaga gaacgtaccg
     4621 ccgcagatga acaggatgcc actggtgtcg atctgcacat actcctgctg cgggtgcttg
     4681 cgcccgccct ggggaggcac gttggccacc gtgccctcga ggatcttgag cagggcctgc
     4741 tgcacgccct cacccgacac gtcgcgggtg atggacgggt tatcgctctt ccggctgatc
     4801 ttgtcgatct cgtcgatgta gacgatgcct cgctggcacg cttcgatgtc gtgatccgca
     4861 gcgatcagga gtttgaggag gatgttctcg acatcctcgc ccacgtaccc ggcctcggtc
     4921 agggcggtcg agtcggcgat ggcgaagggc acgtcgagga tgcgcgccag cgtctgggcc
     4981 agcagcgtct tgccgcatcc ggtgggaccg atcagcagga tgttgctctt gcccagttcg
     5041 acgtcggggt cggcgtcgag catggcgatc cgcttgcagt ggttgtacac ggccaccgac
     5101 agcgtcttct tggccagttc ctggccgacc acatactggt cgagcacacg cttgatgtcc
     5161 tgggg
//
LOCUS       DAOSPL010000098         5157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_16244_length_5157_cov_3.373775, whole genome shotgun sequence.
ACCESSION   DAOSPL010000098 DAOSPL010000000
VERSION     DAOSPL010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16244_length_5157_cov_3.373775"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2102)
                     /locus_tag="PLQ54_02880"
     CDS             complement(<1..2102)
                     /locus_tag="PLQ54_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92232.1"
                     /translation="MSKVWRQSGATTQGASLEQRRAPRYASALLCAALLLLPLLATPA
                     RPQFEDLPEQLAVEFGEVPAVGGNAPAIPGRGGDRGRNRFGGSATGMGLFESQDNDDV
                     FGGLPLGADEYTRRTSSTSKSLIPSSYDYAGYGYGSDGGYGLSGSGTGARGTYRRGGR
                     NQYVLGEKIKWSGSASYAARYNHVTGDAEQFTNQFTDINATEPNASLFWAGKLFGPIY
                     STGNVRLGPYTPTVADWSVSYLQKHYGATYGDLTVDMGRSPFVRLQQSIAGFELNGEF
                     GDSRATFFTAGQKGRVRTDTIAGEGTSGPYRLQYAPIRDGSESVRVDGQTYTQAQYNL
                     DYDTGLLTFEDGIVIATTSVIVVKYEENNSSSTQGQYYGLDVSTRIGKIPLGLVYLSQ
                     EAASSRTSGTTSLQQTETFFGNNTIGPFILSRRPIDFTKPIEVYVDGIARYQSQDWEI
                     NATSGAIIFYTIVSATATVIVKYYYINEDQEASTGLKLLGFDTGVPFTNGAIALSYGH
                     SRSSSASGNAFKARGTYDLLDGRVHVDAGYIYMTPTFQRLGTTSFDRDRQGLDLSIDA
                     EPIDDLRVWFDTTRDRSDTSYSTGASSGGIGSSTSTTDVTNRVFNVTTSRWSGGFDLS
                     RRSWPDIQFNMSRINIARPTWGQSYVGTESGSVSHRFGKRLSVGAAFDQTARQFTPVT
                     ASSSSDTSSTTTTTEPEDT"
     gene            complement(2217..3290)
                     /gene="csaB"
                     /locus_tag="PLQ54_02885"
     CDS             complement(2217..3290)
                     /gene="csaB"
                     /locus_tag="PLQ54_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HOS92233.1"
                     /translation="MTRFLFVGYFGYGNLGDEAVLEALVGRLRERAPEAQVAVVSSDP
                     DATSERLGVEAVPRSPLVGVLRAVTSADVFVMGPGGVLQDATSARSILYYSGLSEAAR
                     QAGKRVCLVGNGLGPLRTKLGRLATARVVRGASCLAVRDTESARLARELGARVEPEVT
                     ADAALMLRPDVDAAARLVSHLGLEGETNVVGVSLRLWRGSATAPQWAETLASVRKTLG
                     CRFLWLPCGGEEDERLLLEVARLMGGDDPFWHPPLAPRVFLGLCHHLRGLVGMRLHSL
                     IFATSASRPVAGIAYDPKVSYFLSQLGVEPAIELSREPVAPRLWADRVCRALERDPES
                     YATQIAQMQGRSDRNCELLLQYV"
     gene            complement(3293..5140)
                     /locus_tag="PLQ54_02890"
                     /pseudo
     CDS             complement(3293..5140)
                     /locus_tag="PLQ54_02890"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
BASE COUNT          855 a         1606 c         1850 g          846 t
ORIGIN      
        1 gtgtcctcgg gttccgtggt cgtggttgtt gatgaggtgt cgctgctgga ggaagcagtc
       61 accggagtga actggcgtgc cgtctggtcg aaggccgcgc cgacggagag tcgcttgccg
      121 aaccggtgcg agaccgagcc gctctcggtc ccgacgtagc tctggcccca ggtgggccgg
      181 gcgatgttga tccggctcat gttgaactgg atgtcgggcc agctcctccg ggagaggtcg
      241 aacccacctg accagcggct cgtcgtcacg ttgaagacgc ggttggtcac atcggtggtc
      301 gacgtgctgc tgccgatccc tccgctgctg gcgcccgtcg agtaggaggt atcggaccga
      361 tcccgtgtgg tgtcgaacca tacacgcagg tcgtcgatgg gctcggcgtc gatgctcagg
      421 tcgagtccct ggcggtcgcg gtcgaagctg gtggttccca ggcgctggaa ggtgggcgtc
      481 atgtagatgt agccggcgtc gacatgcacc ctcccgtcca gcagatcgta cgtgccgcgg
      541 gccttgaacg cattacccga tgccgagctg gagcggctgt ggccgtatga gagggcgatg
      601 gcgccgttgg tgaatggcac tccggtgtcg aatccgagaa gcttcaggcc tgtgctggct
      661 tcctggtctt cgttgatata gtaatacttg acaataacgg tggccgtggc cgagacgatg
      721 gtgtagaaga tgatggcacc gctggttgcg ttgatctccc agtcctggga ctgatagcgc
      781 gcgatgccat ccacgtagac ctcgatcggt ttggtgaagt cgatcgggcg ccggctgagg
      841 atgaacggcc cgatggtgtt gttgccgaag aaggtctccg tctgctgcag cgatgtggtc
      901 cccgatgttc tgctcgatgc tgcctcctgc gacagataga ccaggcccaa ggggatcttc
      961 ccgatccggg tcgagacgtc gaggccgtag tactggccct gagtgctcga tgagttgttc
     1021 tcctcgtact tcaccacgat gaccgaggtt gtcgcgatga cgatgccatc ctcgaaggtg
     1081 aggagtccgg tgtcgtagtc gaggttgtac tgggcctggg tgtaggtctg gccgtcgacg
     1141 cggacggact cggatccatc gcggatgggc gcgtactgga gtcggtaggg tcccgaggtg
     1201 ccctcgccgg cgatggtgtc ggtccggacg cggcccttct ggccagccgt gaagaaggtg
     1261 gcccggctgt ccccgaattc gccgttgagc tcgaaacccg cgatggactg ctggagtcgc
     1321 acgaaggggc tgcgccccat gtcgacggtc aggtcgccat aggtggcgcc gtagtgtttt
     1381 tggaggtatg acacggacca atcggccaca gtgggcgtgt aggggcccag tcgcacgttg
     1441 cccgtgctgt agatgggccc gaacagcttc ccggcccaga acaggctggc gttgggctcg
     1501 gtcgcgttga tgtcggtgaa ctggttggtg aattgctcag catcgccggt gacgtggtta
     1561 tagcgtgccg cgtaggaggc ggacccgctc cacttgatct tctcacccag cacgtattgg
     1621 tttcggcctc cgcggcggta ggtgcctcgg gcgcccgtgc cgctgccgga gagaccgtac
     1681 ccgccatcgg agccgtaccc atagcccgca tagtcgtagc ttgacggtat caaggacttc
     1741 gaggtgctgg atgtgcgccg cgtgtactcg tcggcgccga gcgggaggcc gccgaagacg
     1801 tcatcgttat cctgcgattc gaagagcccc atgccggtgg cgctgccgcc gaaccgattg
     1861 cgccccctat cgccccctcg cccggggatg gccggagcgt tgccacccac cgcgggcacc
     1921 tcgccgaact ccaccgccaa ctgctcgggc aggtcctcga actggggacg tgcgggcgtg
     1981 gccaggagcg gcagcaacag aagagcggcg cacagcagag ccgaggcgta gcgaggagcc
     2041 cgccgctgct cgagcgacgc gccctgcgtg gtggctcccg actgtctcca gaccttgctc
     2101 aaccggtccg accccgtaca cggctagccg gggccacata agcgccgccc tgtcgccctc
     2161 ggcagtaatg gcagccaggc aacccgatgg caatgtgccc caagagcagc ctcttctcac
     2221 acgtactgca agagtagttc gcagttgcgg tctgaacgtc cttgcatctg agcgatctgc
     2281 gtggcgtatg actcggggtc gcgctccagc gcgcggcaga cgcggtcggc ccagagacgg
     2341 ggcgcgacgg gttcccgcga gagctcgatg gcgggttcga cgcccagttg tgagaggaag
     2401 tagctgacct tagggtcata ggcgatgccc gccactggtc tgctggcgct ggttgcgaat
     2461 atgagcgaat ggagcctcat tcccaccaga ccccgcaggt gatggcacag ccccaggaag
     2521 actcgggggg ccaggggagg atgccagaag ggatcgtccc cacccatcag acgcgcgacc
     2581 tcgagcagaa gtctctcgtc ctcctcgcct ccgcagggca gccagaggaa acggcaaccc
     2641 agggtcttcc gtacgctggc cagcgtttcg gcccactggg gggcggtggc ggagccgcgc
     2701 cagaggcgga ggcttacgcc caccacgttg gtctcgccct cgagcccaag gtggctgact
     2761 agccgggctg cggcgtcgac atcgggccgg agcatgaggg cggcgtcggc ggtgacttcg
     2821 ggttcaaccc ttgccccgag ctcgcgggcg aggcgggcgg attcggtgtc gcgcacggcg
     2881 aggcaggatg cgccgcggac cacgcgggcg gtggccaggc gccccagctt cgtccgcagc
     2941 ggtccgaggc cgttgccgac caggcagaca cgcttcccgg cctggcgcgc cgcctcggaa
     3001 aggcccgagt agtagaggat ggaccgggcg ctggtggcat cctgcagcac acccccgggt
     3061 cccatgacga agacatcggc cgaggtgacg gcgcgcagga cgccgacgag tggtgaacgg
     3121 ggaacggctt cgacgcccag ccgttcagat gtggcatcgg ggtctgatga caccacggcg
     3181 acctgggctt cgggggcgcg ttcgcgtagg cggccgacca gagcctcgag aacggcctcg
     3241 tcgcccaggt tcccgtagcc gaagtagccg acgaagagga agcgcgtcac agctagccgg
     3301 tgtcccctcc cgtgggttgg gccgagctga ggtgctcgtc cgcgtggcgg ctcgaccggc
     3361 cgcctgtcca tcgtcgagcg gcggtgacca cgatgcaggc ggccagcccg atgaggaggc
     3421 cgagggcgat gccatagccg gtccgtgtca gagtcgcggc gatgggcgtg tggatgtggc
     3481 agaaggtgtt aacgagtgaa gcctgaccaa cggccgcgat gaccagcagg ggcacgacga
     3541 aggtgcgccg gcgagatagg cccaggaagg cacccaagac gagtgccggg tgtccgagca
     3601 tgaactcctt agtgcggggc cggtatacga gcagcgactc gagtagatcg cggaggcccc
     3661 gctcgctctc gagcgttgcc gacgcggcct ggttgcccgt ccgcatgatc cagtaggccc
     3721 ccgcacccag gaccagcacc ccgatgagag catagcccac ggtgaacggc gtaccccagg
     3781 taccgcccaa gcggcgccgg gcgccctgcc accactcggg ccaggtgcat cccgtgccgc
     3841 tgagccggag gacgaggaca gtggccacgg tgatcacggg cacaagctgc gagagcttga
     3901 ttccccggaa ctggagcgcc ttggtcatga aggcggagtc ggagagcagt gcggccgcga
     3961 tcaggccgcc cagtgtggag aacagcgacg ccgccacgaa cagcccggcg gactgggcca
     4021 ggtgccggcg gaggcccccg ccgcccctgc ggatggagcg ccacacgtag accacggcga
     4081 gagtggggaa ggtgatgcat gtgacccacg ccgcgatctg gcggccggcg gtggccgaga
     4141 cggcggtcag tccgcagaac gcgatggtgg cgagggcggc gccgaggatg agtgccggac
     4201 cggacacggg ggcgatgagc gcaaccagga gcaccaggcc cccggccgcg ccgagggcca
     4261 ggaccaggcg ggcgtccgct ccgcccgtga agcgggggaa acgctccgcc ataccgagcg
     4321 ggtaacccgc gtcctggagg tccttggcca gtgcggccac atagctcaga ttgtagtcca
     4381 gtgggtcatc catggcccgg tccaggaaca ggtgaacgaa gcagcagcgc acgttgcgct
     4441 cgcgaacggc gcggacgaag cgcctgcgcg ccgcgtcggg tgagagtttg agcatctcct
     4501 tgtcggcgac ggagtgggtg cggaccactg cggacttcag caggtgagtg agggcaccgg
     4561 tgccttcctg aacgtcgagt tcgacggcgt agaaggtcag gtcgtttgcc cggaacagtt
     4621 cagcggtcag cggcagcagg tcggggttcc cgagcacgaa cttgccggcg aacatgatcc
     4681 gatccattcc ggcctacttc gcctgcgtca gcgtgtaggt caatccccga cggtcgatgc
     4741 gcgcggagtc gatggggcgc gctacgggag tcagtttggc ggcgcgcacc tgggcgatct
     4801 gctcggcctg ccagccgacg ccgatgtcct tcagggccag cagagggacc ccaaactcga
     4861 aggtcctggg gacatcggtc cccggctccc actggtcgga cggccatttg gcctcaaggc
     4921 tgcggcggac acgttcggcc cattgggggt agctgacgcc aagcaggaga tgcccggccc
     4981 cttcgaggac cgatgcgcga ccggcgtcga ccatgccggg gagggtctcc tcgttgacgg
     5041 cgatgccggt cacgccgact ttctgtaggc gtgcgagcag gtcgggtgcc gacttgcctc
     5101 ggctctcggc gagagccgct acctcgtcgt agtccagcac cacctcaacg cgccggt
//
LOCUS       DAOSPL010000099         5140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_16327_length_5140_cov_3.333137, whole genome shotgun sequence.
ACCESSION   DAOSPL010000099 DAOSPL010000000
VERSION     DAOSPL010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16327_length_5140_cov_3.333137"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1674)
                     /locus_tag="PLQ54_02895"
     CDS             complement(<1..1674)
                     /locus_tag="PLQ54_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92234.1"
                     /translation="MGPRSRRLAVFLAAALMALAQGCDTGKGTAEPREAAKPQAGIEL
                     TNEVGEKVDAFPVGAAAFVTLTNLTPYQPYDITLYAPAASAIESAQAAAEANRAQEMK
                     DTKGHGPSEGGPSGQPAGGAPASSRPPGPTTEAAGPRHVVAGPEDAAPPADAPVAAEC
                     AAPGPVPPDVPAPALGEPTTKAGEAGGSPADKAAAAQAEAAQVPAMPPAPQGTVVGGG
                     VFMSDAEGKIIPTLIARDLGLTSQLPAGKYTLSVKSVGGVDMGSKTFDVVESKDPYCY
                     PSTPDGTLKNAFQADAETLYVRAENLPPQAEVDIYVVADAGKYENGQALKDVSGSITY
                     AQADDRGHVLIDVWTGCPQPGAYDIFIDLNNNGKLDTEVDLANGVKTRDVLYGFRTVG
                     ALVQEKPVVPGEMDLVAELGCAEDVYEKGPAPRVGSDKALCVRAAPTLVGATPMAGEA
                     SIYIVRHQDTWKTGDKLENKGPGPTKVRTVGAWGMDRVRVWPAPVLPGAYDVVVDVDN
                     NGAYDRGIDLLDNTGTQENGVSVGMVVTDAGNLVTLAGRVVDSSGNPVGG"
     gene            1984..3537
                     /locus_tag="PLQ54_02900"
     CDS             1984..3537
                     /locus_tag="PLQ54_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOS92235.1"
                     /translation="MSILPFPGLHPPAMPTSELPQGWLALVGDLVPPAVFEYALAALV
                     LWVPLGGAVLAVLLPPRSGARMGRWAALVCVLLVGLLALSPAVTRGASVSLYPHGGVR
                     CATLDALGWLVCLLTAVALLVAVWWDRPAARQQEEPTPALDSWLLVCAFTAFAMATAS
                     ATPEVAALLAGVGLMPALYVVLGLPYGGRRRNVIRPAVAGLLSGALFLAGTQISDMGR
                     ARGAPLDSIFGLSHTAVLGLIVIIGAAYAGAAMFPFCLWPSRIALSPGRREAAFLLGS
                     TVFLPGIIALARVMYVATGRVWRLQWSVVEGLGVVYLLLVGFTVAYLFIEVIDEWDVG
                     RRFGVLASVHGAYLFAAVIAYTVSVGSGAQALVYGLVARTIEFGLIFLSLDWLAPELR
                     HARGLDGLRSAAERYPFVALGLVCAMASLAGLPPFIGFWSKWVLARALTDAFGSYGLG
                     IWAVYAVELMLLASWVVLPGRGKASAARYAEPWRDMPTVKRAIIGGLTLLTVAAGPAV
                     VPVLAMIHR"
     gene            3640..4119
                     /locus_tag="PLQ54_02905"
     CDS             3640..4119
                     /locus_tag="PLQ54_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOS92236.1"
                     /translation="MSAREVMVTDLVTARGEETAGAVALRMLEHDLAAVPVVDDEDHI
                     LGVVSDVELIRLVVPAYMDLLEDTDFLPDDFEPFERRATAAGATLVRDIMSDQFETVS
                     EDTSLPSIAAHLVRPGVRRVFVLRDGRLVGAIGRRDVFATMFDRAHARTGVDHGDLL"
     gene            4183..>5140
                     /locus_tag="PLQ54_02910"
     CDS             4183..>5140
                     /locus_tag="PLQ54_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOS92237.1"
                     /translation="MPEPLLKPLAGAIFLLCYVAIASDRIDKTKVALFGAAAMLVLGV
                     VTQREAFGHYEFREEIIRHFVDESAGPEGAVSEELPGGARSLPGADWNTIFLLVGMMV
                     MVGIMRHTGIFEWLAIKCAKAARGDPLRIMILLSVVTAVLSAALDNVTTVLLIAPVTL
                     VIADALGISALPLLIPEILASNIGGTATLIGDPPNIIIGSKAGISFTEFVTNLGPTIA
                     IVLAVFLATARFVYRKALTVPEERRAEVMELDETKSITNHRLLWRCLGVMTLVLLGFG
                     LHARLGWEPATVALGGGALLLLLSHLEPEEAFREVEWPTIFFF"
BASE COUNT          671 a         1697 c         1771 g         1001 t
ORIGIN      
        1 gccgccaacc gggttcccgc tgctatcgac cacgcggccg gcgagagtca ccagattacc
       61 cgcgtcggtg acgaccatgc cgaccgacac gccgttctcc tgcgtgccgg tgttgtcgag
      121 caagtcgatg ccgcggtcat aggcgccgtt gttgtcgaca tccaccacca cgtcatacgc
      181 accgggaagg acgggggcgg gccagacgcg gacgcggtcc atcccccagg cgccaacggt
      241 gcggaccttg gtcggccccg gacccttgtt ctcgagcttg tcgcccgtct tccaggtatc
      301 ctggtggcga acgatgtaga tcgatgcctc cccggccatg ggtgtggcgc ccaccagagt
      361 cggtgccgcg cggacgcaca gcgccttgtc gctgcccaca cggggagcag gccccttctc
      421 gtagacgtcc tcggcacacc caagctcggc caccaggtcc atctcgcccg gaaccacggg
      481 cttctcctgg accagcgcgc cgaccgtccg gaagccgtag agcacgtccc tggtcttcac
      541 gccattcgcg aggtccactt ccgtgtcgag cttcccgttg ttgttcaggt cgatgaagat
      601 atcgtaggcg ccaggctgcg ggcatccggt ccacacatcg atcagcacgt ggccccggtc
      661 gtcggcctga gcatacgtga tcgagcccga aacgtccttg agcgcttggc cgttctcgta
      721 cttgcccgcg tcggccacca cgtagatgtc gacctcggcc tggggcggca ggttctcggc
      781 ccggacgtag agcgtctcgg catccgcctg gaaggcgttc ttgagagtgc catcgggtgt
      841 cgacggatag cagtacgggt ccttgctctc caccacgtcg aaggtcttgg agcccatatc
      901 gacgccgccc acagacttca ccgagagcgt gtacttgcct gctggcagct ggctggtcag
      961 gcccagatcg cgagcgatca gcgtcgggat gatctttcct tctgcatccg acatgaacac
     1021 gccgccgccc acgaccgtcc cctgaggggc cgggggcatg gccggtacct gggcggcttc
     1081 ggcttgagcg gcggccgcct tgtcggcggg tgatcccccc gcctctccgg ccttggtggt
     1141 gggctctccc agagccgggg cgggcacatc gggcggtacg gggccgggcg cggcgcactc
     1201 ggccgccacc ggggcgtcgg cgggcggcgc ggcatcttcg ggtcccgcaa ccacatggcg
     1261 cggtcctgcg gcctcggtcg tgggccccgg cggccgggag gatgccgggg ctcctccggc
     1321 cggctggccc gacggtccgc cttcggaggg gccgtggcct ttggtatcct tcatctcctg
     1381 agcacggttc gcctcggcgg ctgcctgggc cgattcgatg gcggaggcgg cgggtgcgta
     1441 cagcgtgatg tcatagggct ggtacggtgt gaggttcgtg agggtaacga aggcagccgc
     1501 gcccaccggg aaggcatcga ctttctcgcc cacttcgttg gtgagttcga tgcccgcttg
     1561 tggcttcgcc gcctctcggg gctcagccgt gcccttgccc gtgtcgcatc cctgggccag
     1621 ggccatcagc gccgccgcga ggaatactgc caggcgcctc gatcggggac ccatttccga
     1681 tgcctcccgc ccggtgccgc gccccactgg ggccggcacg tctcggccaa gcaatgacag
     1741 tcagcgggcg cggccaaaac gcgctggtag cccctgcccg aaacctctaa gatggatgtc
     1801 gcttgggggt tccgcccggt cgggtgtccc agggaatgcg cccaccaagc tatctacgat
     1861 tatacgctcc actgttttgc ccgcgattcg ctttcgccgg ggatatctag gcgtgggagg
     1921 ccctagtttg cgttgccgca ctgctctgtt ggccctcgct gacctctgcg cacgagcgga
     1981 cgggtgagca ttttgccgtt cccggggctc catcctccgg ccatgccgac ctccgaactc
     2041 ccccaggggt ggctcgcgct cgtgggtgac ctggtgccac ccgcggtttt cgagtatgcc
     2101 ttggcggccc tggtgctgtg ggtgcccttg ggcggcgcgg tcttggcggt gcttctgccc
     2161 ccgcgcagcg gagcgaggat gggccgctgg gccgcactgg tgtgcgtgct cctggtcggg
     2221 cttctggccc tgtcccctgc cgtaacgcgc ggtgcaagcg tgagtctcta cccgcacggt
     2281 ggagtgcgct gcgcgaccct cgatgccctg ggctggctgg tgtgcctcct gacggcggtg
     2341 gcgttgctgg ttgcagtgtg gtgggatcga ccggcggcgc ggcaacaaga agagccgacg
     2401 ccggcactcg atagctggct gctggtgtgt gcctttacgg ccttcgccat ggccacggca
     2461 tcggcgacac ccgaggttgc ggccctgctg gcgggtgttg gcctcatgcc cgccctgtac
     2521 gttgtcctcg gtctcccata cggcggacgg cgccgcaatg tgattcgacc cgccgtcgcc
     2581 ggccttctgt cgggcgcgct gttcctcgcc gggacccaga tctccgacat gggtcgggca
     2641 cgtggggcac cgctggacag catcttcggc ctgagtcata ctgcggtctt gggtctgatc
     2701 gtgatcattg gcgcggccta cgcgggtgcc gcgatgttcc ccttctgcct gtggccatcc
     2761 cgcatcgccc tgtcgccggg acggcgtgag gcagccttcc tcctcggatc gacggtgttt
     2821 ctccctggca tcatcgctct ggcccgcgtg atgtacgttg cgacaggccg tgtgtggcgg
     2881 ctgcagtggt cagtggtcga ggggttgggc gtggtctatc tcctgctcgt cggcttcacg
     2941 gtggcctacc tgttcatcga ggtcatcgac gagtgggatg tgggccggcg cttcggggtg
     3001 ctcgccagtg tacacggcgc ctatctcttc gcggcggtga ttgcgtacac ggtctccgtt
     3061 ggctccggcg cccaggcgct ggtgtacgga ctggtggcac ggactattga gtttggcctc
     3121 atattccttt cgctggactg gctggccccg gaactccggc atgcacgcgg actcgatggc
     3181 ctgcgctcgg cagccgagcg atatccgttt gtggccctcg gtctcgtgtg tgcgatggcc
     3241 tctctggctg gtctgccgcc cttcatcggc ttctggtcga agtgggtgct ggcgcgggct
     3301 cttacggacg cgttcggctc gtacgggctg gggatctggg ccgtctatgc ggtggagctg
     3361 atgctcctcg cgtcgtgggt agtcttgccg ggccgtggca aggcctcggc cgcgcgctat
     3421 gccgagccgt ggcgggacat gccaacggtc aagagggcca tcatcggggg gctgacgctt
     3481 ctcaccgtcg ccgcgggccc ggccgttgtg ccggtgctcg caatgattca ccggtaacgc
     3541 ctcgggccgt tacccgcccg gaggctatcc gtacttccca ccggaggaag agccaacggg
     3601 cccgccaggg cgggctcgtc gcgcaggggg gtgctgtgaa tgagtgcccg ggaggtcatg
     3661 gtcaccgatc tggtgactgc gcgtggcgag gagaccgccg gagccgtggc gctgcggatg
     3721 ctggaacatg acctggccgc cgttcccgtg gtcgatgatg aagaccacat cctcggcgtc
     3781 gtgtccgatg tcgagctgat ccggctcgtg gtgccggcgt acatggacct gctggaggac
     3841 acggactttc tgccagacga tttcgagccg tttgagcgcc gggccaccgc ggcgggcgcg
     3901 accttggtgc gcgacatcat gtcggaccag ttcgagacgg tctccgagga cacatcgctc
     3961 ccgtcgatcg cggcccatct ggtcaggcca ggcgtgcggc gcgtgttcgt cctccgcgat
     4021 ggaaggttgg tgggggccat cggccggcgg gatgtgttcg ccaccatgtt cgaccgtgcc
     4081 cacgcccgga cgggggtcga ccacggcgat ctgctctgag cggcgcccga ggtacgatca
     4141 aggcgtgctc cgcggcagcg cgccgggagg aggtggattc ccatgccaga gccgcttctg
     4201 aagcccctgg ccggggccat cttccttctc tgctatgtgg ccatcgcgtc cgaccgcatc
     4261 gacaagacga aggtggcgct gttcggcgcg gcggccatgc tggtgctcgg agttgtcacc
     4321 cagcgcgagg cgtttggtca ctacgagttc cgcgaggaga taatccgcca cttcgttgac
     4381 gagagcgccg gccccgaggg cgccgtttcg gaggaactgc ccggcggtgc gcggagcctt
     4441 cccggggccg actggaacac aatcttcctg ctggtcggca tgatggtgat ggtcggcatc
     4501 atgcgtcata cggggatctt cgagtggctt gccatcaagt gtgccaaggc ggcccgaggc
     4561 gatccgctgc ggatcatgat cctgctctcg gtggtcacgg cagtactgtc ggcagcgctc
     4621 gacaatgtga ccaccgtgct gctgatcgcg ccggtgaccc tggtcattgc cgatgcactg
     4681 ggcatctcgg cgttgccgct gctgatcccg gagatcctgg cctcgaacat cggcggcacc
     4741 gcgacactca tcggcgaccc acccaacatc atcatcggaa gcaaggccgg catctccttc
     4801 accgagttcg tgacgaacct gggcccgacg atcgccatcg tactggccgt gttcctggcg
     4861 acggcgcgct tcgtgtaccg caaggccctt accgtgcccg aggagcgccg agccgaggtg
     4921 atggagctgg atgagacgaa gtccatcacc aatcacaggc tgttgtggcg gtgcctgggc
     4981 gtcatgacgc tcgtgctgct gggcttcggg ctccacgccc ggctcgggtg ggagcccgcc
     5041 accgtcgcgc tgggcggcgg cgccctgttg ttgctcctat cgcacctcga gcccgaagag
     5101 gccttccgcg aggttgagtg gccgacgatc ttcttcttcc
//
LOCUS       DAOSPL010000100         5134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_16363_length_5134_cov_2.868478, whole genome shotgun sequence.
ACCESSION   DAOSPL010000100 DAOSPL010000000
VERSION     DAOSPL010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16363_length_5134_cov_2.868478"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2025
                     /locus_tag="PLQ54_02915"
     CDS             <1..2025
                     /locus_tag="PLQ54_02915"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92238.1"
                     /translation="TLALLPCLASRWVSGSDGRVPANGGIPLGRALAVLACVLSAAPS
                     IATTPADRALAQELTASFSRTTSRVSALVLDRFDRAGVELAAYDRNRVFHYPLDSALK
                     EAPAIGVAELDLGGALVPGSVVDTVVDTPLTANDLAQAMAWRALTKYLSEWDWEASEP
                     LLAAEAKVAGETWGNGRLPKPLQQLAVAYLHEGELWAKVEFRPELTWVRADDEDGDGY
                     AEVYGRVDPGPSAEAILREIRDNYLGPKLSAAEIETVLLELASDWYGELQTIVLDPPL
                     TRPWPNEETEPDVRDLLGGRVFEDAFAVLRSKPYGEPIYNVILLGSGDAVAGPEDGTV
                     PDAGEAFGVGNPWATEPVQFGGTWDAWAQSLGPFTDDVRAQLDARPAELKALIGREGW
                     LFFRGDLEYLLSGELRGQPDERDPYPAIVDFRNQLAAKGIDLLVVFIPTKAEVYPERI
                     SAAAPPGARPYVAPYCRKLMAELAEAGVELVDLLPAFIDARAPAPDALYQDCDAHWTN
                     RAVRLAASRIAERVRRYDWYDGLAGQPEAYTTRPATFRRRGDLCDMLAPDEKLAFRPQ
                     ELTALQVVGPDGQLYVDDPSSPIVVLGDSFTGVFELEDCQHAGLTAHLARELGVPVDL
                     VMAQGSGPTIRGQFVRRGAEAIARKKLVIWTVVSRDLYHYWSPWKSIRVP"
     gene            2026..3495
                     /locus_tag="PLQ54_02920"
     CDS             2026..3495
                     /locus_tag="PLQ54_02920"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOS92239.1"
                     /translation="MVFPSHVFLFYFLPVALGLYYLCPARWHGLLLIGLSYLCYGWAN
                     PWFVLLLVFSTVLNYACGEWISGRWRMPWLWRGEPAGRGLPATRQRKAALGLCIGGNL
                     AFLGFFKYLGFVESGLNVMLGWFGGASVHFLTVALPVGISFYTFKAMSYGLDVYQGAT
                     KPARSLLDFANYVALFPQLVAGPIGRFRDLGAQLEGRTHTLEKFCQGVFFFALGMSKK
                     VLLADPMGQVADAAFAAVSVPWHAAWAGALAYSFQIYFDFSGYSDMATGLGLMMGFEF
                     RRNFDLPYLSQSITEFWRRWHISLQNWLQDFIFMPLTKRLMRTRLRGKGHAIPWIGYM
                     ITFVACGLWHGAAWTFVAWGGLHGLLLCLERLAGRKPIYAGLPPWCRTLATFALVLLS
                     WVLFRAETLGGALSYLGAMFGLTDAGPSAALVPGLVYQPHFLLVFVACLLIHILRLDT
                     WDLAQSLKPAKLVLAAAAFAASVAMLFTQSYEPFLYSRF"
     gene            3508..4692
                     /locus_tag="PLQ54_02925"
     CDS             3508..4692
                     /locus_tag="PLQ54_02925"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92240.1"
                     /translation="MTDGRWQMTGDTRQTADGGGSAPVSGVWHILLVGVFLASIVALP
                     VSQVAIECARGEAVRELRVFARFPTAESLAEYERGLERDSYVGAAVRRAVQRRLFGAL
                     HAGNEKCVVGRDGALFYRPSIDSVVAPGFMAGPQAEGHPVRAITAFRDALSTQGVELL
                     LVAVPGKESVHPEWLAPGYPAEGPPPANVDLPAFMQALGEHGVRCIDPTGALWAAKAH
                     GPMYLRRDTHWSPAGLNVVADLVAQAVPPELRGESRFDVERLTIGHGGDLHDMLDIGR
                     PPVVETVRIDRVIDESTGEPVEPDARSPVVLLGDSFTNIYSVPDLGWGDHAGLAEQLA
                     LRIGRRIDVIALNDGGVNTSRASLCRRPHPLEGKRLVIWQFAARDLVLANGNWKTMRI
                     RR"
     gene            4707..>5134
                     /locus_tag="PLQ54_02930"
     CDS             4707..>5134
                     /locus_tag="PLQ54_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92241.1"
                     /translation="MRSSRIATGLLVLLPVLALGAATGCGRKDGDGADAPVAAPRMPL
                     AAPKTSPPSGVAEGVDGPDGASPEAGAAAPTRPDEPVAVGDVQPGSLGPTTGDSDRRA
                     PGATPGEGPSQRAEPKAAAGDGLVVVGTVELVSHVPKPGEA"
BASE COUNT          720 a         1637 c         1850 g          927 t
ORIGIN      
        1 accctcgcat tgctgccgtg tctagcttcc cgatgggtgt ctggctccga tggccgtgtg
       61 cccgcgaacg gagggatacc gctgggtcgc gcactcgccg tcctcgcgtg tgttctttcg
      121 gcggcgccgt ccatcgccac aacaccggcc gaccgggcgc tggcccagga actgaccgca
      181 tcgttctcgc gaactacatc gcgcgtgtca gcgctggtgc tggaccggtt tgaccgcgcc
      241 ggcgtggagc tggcggccta cgaccgcaac cgtgtctttc actacccgct cgactccgcg
      301 ctcaaggaag cgccggctat cggcgtcgcc gagttggacc tgggtggcgc gctggtgccg
      361 ggctcggtcg tggacacggt agtcgatacg cccctcaccg cgaatgacct ggctcaggca
      421 atggcctggc gggcattgac caagtacctc tcggagtggg actgggaggc ctcggagcct
      481 ctgctggcgg cggaggcgaa agttgcaggc gagacctggg gcaacgggcg cttgccgaag
      541 ccgctccagc agctcgctgt ggcctatctg cacgagggcg agctgtgggc gaaggtcgag
      601 tttcggccgg agctgacatg ggtccgcgcc gacgacgaag atggcgacgg ctacgctgag
      661 gtgtacggca gggtggaccc gggcccgtcg gccgaggcga tcctccggga gatccgcgac
      721 aactacctcg gccccaagct ctcggcagcc gagatcgaga ccgttctcct cgagctcgcc
      781 tcggactggt acggtgagct tcagaccatc gtgcttgacc ccccgctgac tcggccctgg
      841 cccaacgagg agacggagcc ggatgtccgg gatctgctcg gtggccgtgt cttcgaggac
      901 gcctttgctg tgttgcggag taagccgtat ggcgagccga tctacaacgt catcctcctg
      961 gggtcggggg acgcggtggc cgggcccgag gatggcaccg tgccggatgc gggagaggca
     1021 ttcggtgtgg ggaacccgtg ggcgacggaa cccgtccagt tcggcggcac gtgggatgcg
     1081 tgggcgcagt cgctgggacc gttcacggac gatgtgcggg cgcagctgga tgcgcgcccc
     1141 gctgagctca aggcgctgat cggacgcgag ggctggctgt tcttccgcgg tgaccttgag
     1201 tacctgctgt ccggggagct acgcggccag ccagacgaac gggaccccta ccctgccatc
     1261 gtggacttcc gcaaccagct cgccgccaaa ggcatcgacc tgctggtcgt gttcatcccg
     1321 accaaggccg aggtctatcc cgagcggatc tcggcggctg ctcccccggg ggcgcggccc
     1381 tatgtggcgc cctactgccg gaagctgatg gccgagctgg cggaggccgg tgtcgagctc
     1441 gttgacctgc tgccggcgtt catcgatgcc cgagctccgg cgccggatgc cctctatcag
     1501 gactgcgacg cccactggac caatcgcgcg gtccgtctgg cagcctcgcg gatcgccgag
     1561 cgggtgcggc gctatgactg gtatgacggg cttgcaggcc agccggaggc ctacacgact
     1621 cgtccggcca cattcaggag acgcggcgac ctgtgcgaca tgctggcgcc tgatgagaag
     1681 ctcgcgttcc gccctcagga gctcaccgct ctgcaggtgg tcggtccaga cggtcagctc
     1741 tatgtcgacg atccctcgag cccgatcgtg gtgttgggcg acagcttcac cggcgtcttc
     1801 gagctggagg actgccagca cgcggggctg acggcgcatc tggcccggga gctcggtgtg
     1861 cctgtcgatc tggtcatggc ccaggggagt ggccccacga tccgagggca gttcgttcgg
     1921 cggggcgcgg aggcgattgc caggaagaag ctcgtgatct ggaccgtggt gtcgcgcgac
     1981 ctctaccact actggtcgcc gtggaagtcg atccgggttc cgtagatggt cttcccgtcc
     2041 cacgtgttcc tgttctactt cctgccggta gctctagggc tgtactacct ctgtccggcg
     2101 cgctggcatg ggttgttgct catcggcctg agctacctct gctacggctg ggcgaacccg
     2161 tggttcgtgc tattgctcgt gttctcgacg gtgctcaact acgcgtgtgg cgagtggatc
     2221 agcgggcggt ggcggatgcc gtggctttgg cgaggcgagc ccgcgggccg gggcctgccc
     2281 gccacgcggc agcgcaaggc ggccctcggc ctctgtatcg gcggcaacct cgcattcctg
     2341 gggttcttca agtacctggg gttcgtggag tccggcctca atgtcatgct ggggtggttc
     2401 gggggcgcgt ccgtccactt tctcactgtc gccctgccgg tgggcatctc gttctacacg
     2461 ttcaaggcga tgagctacgg tctggacgtg taccaaggcg cgaccaagcc cgccaggagc
     2521 ctgctggatt tcgccaacta tgtggcgctg ttcccccagc tcgtggcggg gccaatcgga
     2581 cgcttccggg acttgggcgc gcagcttgag ggacgcaccc acacactcga gaagttctgc
     2641 cagggcgtgt tcttcttcgc cctcggcatg tcgaagaaag tgctgctggc cgacccgatg
     2701 ggccaggttg ccgatgcggc attcgcggcc gtatcggtac cgtggcatgc ggcctgggct
     2761 ggggcgttgg cctactcgtt ccagatctac ttcgacttct cgggctactc ggacatggcc
     2821 acggggctcg ggctgatgat gggttttgag ttccgccgca acttcgacct cccctacctc
     2881 tcccagagca ttaccgagtt ctggcgccgc tggcatatct cgctccagaa ctggctgcag
     2941 gacttcatct tcatgccgct cacgaagcgg ctgatgcgta cgaggctgcg ggggaagggc
     3001 catgcgatcc cgtggatcgg ctacatgatc acgttcgtcg cgtgcggcct ctggcacggg
     3061 gccgcgtgga ccttcgtggc gtggggcggg ctccacggcc tcctgctatg tctggagcgg
     3121 ctcgcgggca ggaagcccat ctatgcaggt ctgccgccgt ggtgccggac gctagcgacg
     3181 ttcgcgctgg tgctcctctc ctgggtgctt tttcgagcgg agacgctggg tggagcactc
     3241 agctacctgg gcgcgatgtt cggtctcacc gatgccggac cgtcggccgc cttggtgccg
     3301 ggtctcgtgt accagcccca cttcctgctg gtgttcgtgg catgcctgct gatccacatc
     3361 ctccggctgg acacatggga cctggcgcag agcctgaagc cggcgaagct ggtgttggcg
     3421 gcagccgctt ttgcggcatc ggtggccatg ctgttcacgc agagctatga gccattcctg
     3481 tactcccggt tctagggcag acggcagatg acagatggca gatggcagat gacgggcgac
     3541 acacggcaga cggcagacgg cggcgggagc gcgccagtca gcggggtgtg gcacatcctg
     3601 ctcgtggggg tgttcctcgc gtcgattgtg gccctgcccg tgtcgcaggt ggccatcgag
     3661 tgcgcccggg gcgaagcggt tcgggagctt cgcgtgtttg cgcggttccc gacggctgag
     3721 tcgctcgcgg agtacgagcg tgggctcgaa cgggactcct acgtcggagc cgcggtgcgt
     3781 cgtgcggtcc agcggcggct gttcggggcg ctccacgcgg gcaacgagaa gtgtgtggtt
     3841 gggcgcgatg gggcgctgtt ctaccggcca agcatcgaca gcgtggtggc cccgggcttc
     3901 atggccggcc cccaggcgga gggtcacccc gtgcgtgcga tcaccgcgtt tcgcgatgcc
     3961 ctgtccacac agggggtcga gctgctgctg gttgccgttc cgggcaagga gagcgtgcac
     4021 cctgaatggc tagcccccgg gtacccggcc gaggggccgc ctccggcgaa tgtggaccta
     4081 ccggcgttca tgcaggccct gggcgagcac ggcgtacgat gcatcgaccc caccggggca
     4141 ctctgggcgg ccaaggcaca cggcccgatg tacctgcggc gcgacacaca ctggtcgccc
     4201 gcgggtctga atgtggtagc tgacctcgtg gctcaggcgg tgccgcccga gttgcggggc
     4261 gaatcgcggt tcgatgtcga gcgtctgacg atcgggcatg gcggggacct gcacgacatg
     4321 ttggacatcg gtcgaccgcc ggtcgtcgag acggttcgga tcgaccgtgt gatcgacgag
     4381 agcacagggg agcccgtcga gccggacgcg cggtcgccgg ttgtgctgct gggcgacagc
     4441 ttcaccaaca tctactcggt gcccgatctg ggatggggcg accacgccgg actcgcggag
     4501 caactcgcgt tgcggattgg ccggcggatc gacgtgatcg cgctcaacga cggtggcgtg
     4561 aacacctcgc gcgcgagcct gtgccggcgg cctcaccccc tcgagggcaa acggctggtc
     4621 atctggcagt ttgccgcgcg ggacctggtt ttggcgaacg gcaactggaa gaccatgcgt
     4681 atccgtaggt agcagaggag atgccgatgc gcagttcacg cattgcgaca ggcctgctgg
     4741 tgctgctgcc cgtgttggcc ctgggggccg caacgggctg cgggcggaag gacggcgatg
     4801 gagccgatgc gccggtggcg gccccccgga tgccactggc ggcgcccaag acttcgccgc
     4861 cttcgggggt cgccgagggc gttgacggcc cggacggggc gagccccgag gccggtgcgg
     4921 cggcgccgac ccgcccggac gagcccgtgg ccgtggggga tgtgcagccg ggtagcctcg
     4981 gccccacgac cggtgactcc gaccgtaggg cgcccggcgc gacgccgggc gaaggaccgt
     5041 cccagcgcgc cgagccgaag gcggcggccg gcgatggcct ggtcgtcgtc ggcaccgtcg
     5101 agctcgtgtc gcacgtcccc aagccgggtg aagc
//
LOCUS       DAOSPL010000101         5114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_16452_length_5114_cov_3.252026, whole genome shotgun sequence.
ACCESSION   DAOSPL010000101 DAOSPL010000000
VERSION     DAOSPL010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16452_length_5114_cov_3.252026"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PLQ54_02935"
     CDS             complement(<1..826)
                     /locus_tag="PLQ54_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92242.1"
                     /translation="MALTAAMVKEAAFAAGAGDVGIANIERFANAPRMMHPLNIAPDC
                     RSVITIVQPIPRGTYRGITEGTHWTNYTIYSYNRLNTLYRPLVTYETACFIEDHGHEA
                     VPLYPGVPERPGTRPPVAEGRPAPDINIQVRIAAVACGVGEIGWSKVLLTRKFGPRVR
                     LGTILTDAELEPDPLPEPSLCNRCMRCAHQCPGGAIPLPGERPTVRIQIEDRTYEWAD
                     VHMGRCTLTHHGMNWEASPFLKRDFPGFALDVRNSTLSEEAAYKLTYPLAQARWLPS"
     gene            complement(826..1746)
                     /locus_tag="PLQ54_02940"
     CDS             complement(826..1746)
                     /locus_tag="PLQ54_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92243.1"
                     /translation="MDAAELKDYARQRGADLVGIASADRFASLPPEANPLSIAPECRS
                     VIVLGRRILRGSLRGVEEGTNFGSTYGLFGFQWLEDNFLSRTTYELTLFIESHGYEGV
                     PLFGYSPEGMPKGVPVAPDKPAPNVIVDLEFAAQAAGLGEVGLGGFFLTPEFGTRQRL
                     AMVLTDAELEPDPVADRRLCGDCRACVDACPLGAINADDARPVGVPGHTMWVAAIDWR
                     LCRACPNGAMVGPGRGERPDRIAAACGRACLNQLERADKCANRFEQPFRKRTPWVLDS
                     LHRPVPPGERGYNPASAGCAKASEVGKEGG"
     gene            1893..3044
                     /locus_tag="PLQ54_02945"
     CDS             1893..3044
                     /locus_tag="PLQ54_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92244.1"
                     /translation="MTSCTLLPALAGLGSLALASFAPAEGATVPSATRPDGTPNRAVW
                     MARGTFGVMTHYLIKPEGATPEERTAELNRIVDNFDVHRYVKRIRETGADWVIFTLGQ
                     TTGYLCAPNEYLDSRAPGMTPRRDLVMELAKHLDDAGKRLILYIPSEQNSEAAIQAAL
                     DSASPGYADRYFEFLKTYAVKYGDLVDGWWFDSCGPHEDEYWLRWLTALRAGNPDTAV
                     AFSGAEFCCGGPVNPVCKLEDYHAGEIHLLEDGAIRRDFLPPGGDIIVTPERKLRLRG
                     QEARTYMPDGQFIDGVQWHGLLPLDLTFNPAVPNQFCHYTDRELLAFALAVKSVGGAI
                     TINVPIEFETGEIPEDSHASLVRLGRNLREAAKHGGKGQGNRVLTPWGT"
     gene            complement(3082..3432)
                     /locus_tag="PLQ54_02950"
     CDS             complement(3082..3432)
                     /locus_tag="PLQ54_02950"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOS92245.1"
                     /translation="MTPQPAVSRGDVWLVRLDPVRGHEQGGTRPALVVSADLLNRGAA
                     GLAIVVPLTTRDRGINLHVRLEPPEGGLAQPSFAKCEDVRSISAERLVKPLGAVGRAT
                     MAQVEDRLRILLCL"
     gene            complement(3429..3692)
                     /locus_tag="PLQ54_02955"
     CDS             complement(3429..3692)
                     /locus_tag="PLQ54_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system protein"
                     /protein_id="HOS92246.1"
                     /translation="MASTTVRLAESARQVLRELADQSGDSMSTVLEKAIECYRRQRFL
                     EDANAGFTALRANPEEWRDELEERQAWEATLGDGLDDEGGSDE"
     gene            3856..4641
                     /locus_tag="PLQ54_02960"
     CDS             3856..4641
                     /locus_tag="PLQ54_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92247.1"
                     /translation="MTDGRDGALESALAALERDADATVRTAAAAVKAAKALQKAAKLG
                     ELRRLDALLAAAGAAATAVSQQVDNTMAGWQFDAAEHLASGEFAHELIAAARAQGVAV
                     VEQDGMLYCCPSLVRVLPGELSVRIDKTRETRLRPTVLAAHLKGVQQRPPRFASEAFL
                     DSLHRAYRRVVEPAHFGRVVKLLELYELLTLLPGQAREYTKAEFARDVYLLDRSGSLT
                     TRNGAVATLHASTGTKSPSAVLRTITETGEKREYYGIAFRPAD"
     gene            4670..>5114
                     /locus_tag="PLQ54_02965"
     CDS             4670..>5114
                     /locus_tag="PLQ54_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92248.1"
                     /translation="MQTTDPRAVRPEDWLDVMKREYVSSFVRHGGAAVKVVVPRDDED
                     RHSLIAGLRSSAEEHGFAFASVDAATTKLHLVDRLFHAVARQMPWHALASSLVRRLLA
                     ASGLEVTPQADGVDLAEVARVNGRDVRWLAQDVKRQLERELVGHPG"
BASE COUNT          804 a         1771 c         1771 g          768 t
ORIGIN      
        1 gggaaggcaa ccagcgcgcc tgggccagcg ggtaagtgag cttgtacgcg gcctcctccg
       61 agagcgtgct gttgcggacg tcgagcgcga acccagggaa gtcacgcttc agaaagggcg
      121 aggcctccca gttcatcccg tggtgcgtga gcgtgcatcg gcccatgtgc acgtcggccc
      181 actcgtaggt ccggtcctcg atctggatcc ggactgtcgg ccgctcgccc ggcagcggga
      241 tggcgccacc agggcactgg tgcgcgcagc gcatgcatcg gttgcagagc gagggctcgg
      301 gcagggggtc gggctcgagc tccgcatcgg tgagaatggt tccgaggcgg acgcgcgggc
      361 cgaacttcct ggtcagcagg accttcgacc agccgatctc gcccacgccg caggccacgg
      421 ccgcgatgcg gacctggatg ttgatgtccg gcgcggggcg gccttcggcg acgggcggac
      481 gggtccctgg acgctcgggg acgccggggt agagtggcac cgcctcgtgc ccgtggtcct
      541 cgatgaagca cgcggtctcg taggtcacca gcggccgata gagcgtgttc agccggttgt
      601 aggagtagat ggtgtagttg gtccagtgcg tcccctcggt gatgccgcga taagtccccc
      661 tggggatggg ctgcacgatg gtgatgaccg accggcagtc gggcgcaatg ttgagcgggt
      721 gcatcatccg cggcgcgttg gcgaaccgct cgatgttggc gatgcccaca tcacctgcgc
      781 cagccgcgaa cgccgcttcc ttgaccatgg cggcagtgag agccattagc ctccctcctt
      841 gccaacctca ctcgctttcg cacatcccgc gctggccggg ttgtacccac gctcgcccgg
      901 aggcactggg cggtgtaggc tgtccagtac ccacggcgtg cgcttgcgga agggctgttc
      961 gaaccggttc gcgcacttgt ccgcgcgctc gagctggttg aggcacgctc ggccgcacgc
     1021 ggccgcgatg cggtccggtc gttcgccgcg acccgggccg accatggcgc cgttcgggca
     1081 tgcgcggcag aggcgccagt cgatggccgc cacccacatc gtgtgaccgg gcacgcccac
     1141 cggccgggcg tcatcggcgt tgatggcacc cagcgggcac gcatcgacgc aggcccggca
     1201 atcgccgcac agccgccggt cggccaccgg gtccggctcg agctcggcgt cggtgagaac
     1261 catggcgagg cgctgccgcg tgccgaactc gggtgtgagg aagaagccgc cgaggcccac
     1321 ctcgcccagc cctgcggcct gcgcggcgaa ctcaaggtcg acgatgacgt tcggcgcggg
     1381 cttgtcgggc gccaccggca cacccttggg catcccctct ggcgagtagc cgaacagtgg
     1441 cacgccctcg tagccgtgac tctcgatgaa cagcgtgagc tcgtacgtcg tgcgcgacag
     1501 gaagttgtcc tcgagccact ggaagccaaa gaggccatag gtgctgccga agttggtgcc
     1561 ctcctcgacg ccacgcagcg accctcggag gatccgccgg ccgagcacga tcaccgaccg
     1621 gcactcgggc gcaatggaga gcgggttggc ctcaggcggc agactggcga atctgtccgc
     1681 cgaggcgatg ccgaccagat cggccccacg ctggcgggcg tagtccttga gttcagcggc
     1741 gtccatagcg tcctccgtga gagcagagag gggctcccga ccccttcttc cgcgcagtcg
     1801 ccccgtggcc ttccggcagg ccacgtcggc gccccggacg aacacgccgc tgccgcgggc
     1861 tcgcgggtcg cggctgagag aggaccacga ggatgacatc ctgcacactc ctaccggcac
     1921 tggccgggct tggctcgctc gctctggcgt cgttcgcgcc ggctgagggc gcaacagtcc
     1981 cctcggccac ccggccggac ggcacaccca atcgcgccgt gtggatggcg cggggcacct
     2041 tcggcgtcat gacccactat ctcatcaagc ccgagggtgc cacgcccgag gagcgcaccg
     2101 ccgagctcaa ccgcatcgtc gacaacttcg acgtgcaccg ctacgtcaag cgcatccggg
     2161 agacgggcgc ggactgggtg atcttcacgc tcgggcagac caccggctat ctgtgcgccc
     2221 ccaacgagta cctcgacagc cgcgcgcccg gtatgacgcc gcggcgcgac ctcgtgatgg
     2281 agctcgcgaa gcacctcgac gatgccggca agcggctcat cctctacatt ccgtcggagc
     2341 agaactccga agccgccatc caggccgcgc tcgactcggc ctcacccggc tacgcggatc
     2401 gctacttcga gttcctcaag acctacgcag tgaagtacgg cgacctcgtc gacggctggt
     2461 ggttcgattc gtgcgggccg catgaggacg agtactggct gcgctggctg accgccttgc
     2521 gggcaggcaa ccccgacacc gccgtcgcct tcagcggtgc cgagttctgc tgtggcggcc
     2581 cggtgaaccc cgtgtgcaag ctcgaggact accacgccgg cgagatccac ctgctcgagg
     2641 acggcgccat ccgccgcgac ttcctcccac ccggcggcga catcatcgtg acgcccgagc
     2701 ggaagctccg gctccgcggg caggaagcgc gcacctacat gcccgacggg cagttcatcg
     2761 acggcgtcca gtggcacggc ctgctgccgc tcgacctgac cttcaacccc gccgtgccga
     2821 accagttctg ccactatacg gacagggagc tgctggcctt cgcgctcgcg gtgaagtccg
     2881 tgggtggcgc catcacgatc aacgtgccca tcgagttcga gacgggggag atccccgagg
     2941 atagccacgc ctcgctggtg cggctgggcc gcaacttgcg ggaggcagcg aagcacggtg
     3001 gaaagggcca ggggaacagg gtactgacgc cctggggcac ctaggtgggt gtcgcgcgcc
     3061 cagagcagcg cggagggggc atcacagaca caacaggatg cgcagccggt cctccacctg
     3121 cgccatcgtc gcgcgaccga cggcgcccaa gggcttcacc aggcgctccg ccgagatcga
     3181 ccgcacatcc tcgcacttgg cgaagctggg ctgcgcaagc ccgccctcgg gcggctcaag
     3241 gcggacatgg aggttgatgc ccctgtcgcg cgtggtcagc ggaacgacga tggccaagcc
     3301 tgccgcaccg cggttcagca gatcggccga gacgacgagc gcgggacgcg tgcctccctg
     3361 ctcatggccc cgaactgggt ccagccgcac gagccagacg tcgccgcgtg acacggcggg
     3421 ctgaggggtc actcgtccga cccgccttcg tcatcgaggc cgtcccccag cgtggcttcc
     3481 catgcctggc gttcctcgag ctcgtcgcgc cactcctccg ggttggcccg gagagccgta
     3541 aagccagcgt tcgcgtcctc gaggaaccgt tgccgccggt agcactcgat ggccttctca
     3601 aggaccgtgc tcatggagtc gccgctctgg tccgcgagct cgcgaagcac ttgccgggcc
     3661 gactccgcca accgaacagt cgtgcttgcc atggcccagg cctcctgtct acagaacagt
     3721 atacaccgcc gtagacgcgg caggtagggg gcgcgactcc gcgctctggc cctcggcccc
     3781 ggccctcgcc gggaggagaa ccccgggtct cgcgggaagg tgcgcgaggc tcgtagatgg
     3841 atcggggggc aacggatgac ggacgggcga gatggcgccc tcgagagcgc tctggcggct
     3901 ctcgagcgtg acgccgatgc gacggttcgg acggctgcgg ccgccgtgaa ggccgcgaag
     3961 gcactgcaga aggcggccaa gctcggcgag ctgcgccggc tcgacgcgct cctcgccgcc
     4021 gcgggcgcag cggcaacggc cgtgagtcag caggtcgaca acacgatggc cgggtggcag
     4081 ttcgacgcgg cggagcatct ggcgagcggc gagttcgccc acgaactcat cgcggccgcg
     4141 cgagcgcagg gcgtcgccgt ggtcgagcag gacggcatgc tctattgctg ccccagcctg
     4201 gttcgggtcc tgcccggcga gctcagtgtg cggatcgaca agacccgtga gacccgcctg
     4261 aggcctaccg tacttgccgc gcacctcaag ggtgttcagc agcgtccacc ccggttcgcc
     4321 tccgaggcct ttctcgactc gctgcacagg gcgtaccgcc gcgtggtcga gccggcccac
     4381 ttcggcaggg tggtcaagct gcttgaactc tatgaactac tcacattgct gcccgggcag
     4441 gcgcgcgagt acaccaaggc cgagtttgcg cgcgatgtct acctgctcga ccgaagcggc
     4501 tcgctcacga cccgcaacgg ggccgtggct acccttcatg cgagcaccgg gaccaagtcg
     4561 ccgagcgcgg tgctccgaac catcaccgag accggggaga agcgcgagta ctacggcatc
     4621 gcgttccgac ccgcggacta gcgcagcccg tccgtcctgg aggcatgcca tgcaaaccac
     4681 cgatccgcgc gccgtgcgcc ccgaggactg gttggacgtc atgaaacgtg agtacgtgag
     4741 cagcttcgtg cgccatgggg gagccgctgt taaggtggtc gtgccccgag acgacgagga
     4801 ccggcactcg ctcattgcgg ggctcaggtc ctcggccgag gagcacggct tcgcctttgc
     4861 gtcggtggat gcggcgacca cgaagctcca cctcgtggat cggctgttcc acgcggtggc
     4921 tcggcagatg ccctggcacg ccctggccag ctccctggtg cggcgcctgt tggcggccag
     4981 cggcctcgag gtgacgccac aggccgacgg cgtcgacctg gccgaggtcg ctcgcgtgaa
     5041 cgggcgagat gtccggtggc tggcgcagga cgtcaagagg cagctcgagc gcgagcttgt
     5101 cggccatccg ggcg
//
LOCUS       DAOSPL010000102         5065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_16664_length_5065_cov_3.111178, whole genome shotgun sequence.
ACCESSION   DAOSPL010000102 DAOSPL010000000
VERSION     DAOSPL010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16664_length_5065_cov_3.111178"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLQ54_02970"
     CDS             complement(<1..244)
                     /locus_tag="PLQ54_02970"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOS92249.1"
                     /translation="MVAEVAVVPPGIDLFVSGQGGYRTYRIPALAVTTRGTVLAFCEG
                     RRDGPSDSGDIDIVLRRSTDGGITWGTPQVVVDMGPH"
     gene            complement(263..964)
                     /locus_tag="PLQ54_02975"
     CDS             complement(263..964)
                     /locus_tag="PLQ54_02975"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOS92250.1"
                     /translation="MGRGFTMVELLVCMAIVAVLAAIVLPVVARARESARKTMCLSNL
                     RQISVAFESYVADYDECFPNNGDPYLWMGRRWRWVLQPYMGLTMQHSSIDPSNPNISE
                     RYTPAILVCPSDSTAPAAWDSTSYGYSAAFYHLPSDIQQMTTADLYSGNRFPCVTQKL
                     AHVAHPAHKALVAEWLTNHTPPTVGWWDWRGARNYLFVDGHARFVSARSVLPAMNGFP
                     DINLTRGGVEGQDLP"
     gene            complement(999..5036)
                     /locus_tag="PLQ54_02980"
     CDS             complement(999..5036)
                     /locus_tag="PLQ54_02980"
                     /inference="COORDINATES: protein motif:HMM:NF022100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HOS92251.1"
                     /translation="MSDHLVVRYHVVRAAARIGLALLACGGIGFGQEPNPVTNGGFEL
                     ATPDGLPVDWQIMGTGALVDDAHSGRRAMRLERTADETAPETGMNRAWEAHSGRRDAM
                     LDRRSGGIVFWYKLEAVAPKARVSVYAIPMGAKPLEDTGEQRAEFTIPHDHAGDGKWH
                     EGRLPYDFSANEAVKWVHISPRLVGGAATVLLDDFEWVEKVGPVLRLRRFELVDDAMR
                     PGTVGDLTLTVRNVGDEPTGRLRFEVALPKNLSASRRGSVLEHDALPPGGDATVVWRI
                     TGRREGRSRIRVSGGTQAAPIEATLVCEPVLRLEQLQTQRFILWNGASTAVNCWLTNE
                     GHAIARDVAGELSVPEGFAIRGSATRKLASVAPGQRVALTWTVSATGETPGAHARAAV
                     RAGGELVGELETLLVSGARCTGPPPVAVGAGTNDIMAIVGNDRVRLAFAKASFGYGPA
                     ELYVRGTGAFAYVGAMPRLGRLVVQGDDGPIDVFLTADQVRAEENRLVFTRTGLSVGG
                     SAWDLTWTVTVPPGSNVAEFSASAKSSGAARVLAFEGPMLYAGEGAFGEQKQEALFPG
                     LEWLEGLERSSSTLDIEPGHPDQVRYVPHPNKITIPLMSVYHNGVTVGLLWDARQKWD
                     GVHDRPSAVFASPDYFEGKQSHLMGLFAPSGAGWVKENQRLADTPFELEAGGELRLTA
                     GILARAAATDALVAVDEWIQRYGIPEPHPIPRGSYAAELDFSLQAYLRSLWIEEESIW
                     WSSRGAGPVLCPKGYPPNHLYDLLLGAALTDDAELAAQCRALADRVKGLAGIRALAQD
                     EGLRFGEAAEAQIASGHSSAASLIAGMGPDGGWRFDADQEGTGPFVGMDYHELGADNA
                     LELGLCANRAATLIRHALLTADPAIYQAALRPLELMRQFRVPRAAQVWEVPVHAPDIL
                     AAADAVDAYVAAYGYSGDKAWLADAKRWARAGLPFVYLWSDDSKPFLLYGSIPVFGAS
                     WNKWSWFGRPVQWNGLRYARAVHRLAEYDDSLPWRKIAEGITRSAQYQQSTDDDDIAL
                     WPDSISAIDGSKAAWLFGPNLILWNALPFMGLQPDPRTQVLRTTAGRIHVTATAEVSR
                     PELQGQALRFRVRYPKGDQGLVVVAGVTEPSDVVANERDLQRVPEPQALFGRDEPAWC
                     YVASDARAMIWFPTAGRQLVRLDGVAPKTVQVLPVTLRTIDFGFDQGVEGWVATNGLE
                     PLQVRDGMLVLRATNPDPYMVRSALRIDGSTVPVITVRMKVSAGTQGQLYWVTETAPS
                     YSEDRVVRFELKPGDEFVEYRIVVGGHRLWDGQVITGIRFDFTNGVPDATALVDYIRG
                     TVSGGTTAPVAEPDNVAIPGDESGL"
BASE COUNT          873 a         1812 c         1610 g          770 t
ORIGIN      
        1 tgtgggggcc catatctacg acgacctgcg gtgttcccca tgtgataccg ccatcggtgc
       61 tgcggcgcag cacaatatcg atgtcgcccg aatcggacgg gccatcgcgc ctcccctcac
      121 agaaggccag caccgtgccg cgagtcgtga cggccagggc ggggatccgg taggtgcggt
      181 agccgccctg gcctgagacg aacaggtcga tgcctggggg cacgacggca acctcagcaa
      241 ccatgatccg cctcccaatg ccctacggga gatcttgccc ctcgacgccg ccacgcgtca
      301 ggttgatgtc agggaacccg ttcatcgccg gcagaacgga tcgggcgctg acgaaccggg
      361 cgtgcccatc gacgaacagg tagttgcgag ccccgcgcca gtcccaccat cccaccgtcg
      421 gcggcgtgtg gttcgtgagc cattcggcca ccagagcctt atgcgccgga tgcgccacgt
      481 gcgccagctt ctgcgtcacg cacgggaagc ggttcccgct gtacagatcc gcggtcgtca
      541 tctgctgaat gtcggacggc aggtggtaga aagcggccga atacccatat gatgtcgagt
      601 cccatgccgc tggggcagtg ctatcgcttg ggcagaccag aatggccggg gtgtaccgct
      661 cgctgatgtt cgggttgctc gggtcaatgg agctgtgctg catcgtcagg cccatgtaag
      721 gctgcagcac ccagcgccac cggcgtccca tccagaggta ggggtcccca ttgttgggga
      781 agcactcgtc atagtcggcc acgtagctct caaaggccac actgatttgc ctcaggttcg
      841 acaggcacat cgtcttgcgg gcgctctcgc gggcccgggc cacaaccggc agcacgatgg
      901 cggccaggac cgcgacgatc gccatgcaca ccagcaactc gaccatcgtg aagccccgac
      961 ccatcgtgcg ccgacacgca ccgggcatat agtgtgggct acagaccgga ctcatctccc
     1021 gggatggcaa cattgtccgg ctccgcgacc ggtgccgtcg tcccgccgct gaccgtgcct
     1081 cggatgtagt ctaccagggc cgtggcgtca gggacgccgt tggtgaagtc aaaccgaatg
     1141 cccgtgatga cctggccatc ccaaaggcgg tggccgccga ccacgatgcg gtactcgacg
     1201 aactcgtcgc ccggcttcag ctcaaaccgc accacccggt cctcggagta actgggagcc
     1261 gtctcggtca cccagtagag ctggccctgg gtcccggccg agaccttcat gcgcaccgtg
     1321 atgaccggca ccgttgagcc atcgatcctc agggccgagc gcaccatgta cggatccggg
     1381 ttcgtcgcac gcagcacgag catcccgtcg cggacctgca gtggctcgag accattggtg
     1441 gcaacccatc cctcgactcc ctgatcgaag ccgaagtcaa tggtcctgag ggtgacgggc
     1501 aggacctgca cggtcttggg ggcaacgcca tcgagccgga ccaactggcg gccggcggtg
     1561 gggaaccaga tcatcgctcg agcatcgctg gcgacgtagc accacgccgg ctcgtcgcgt
     1621 ccaaagagcg cctgaggctc cgggacccgc tgcaggtcac gctcgttggc cacaacatcc
     1681 gagggctcgg tcacccccgc gaccaccacg aggccctggt cccccttcgg atagcgcacg
     1741 cggaagcgga gcgcctggcc ctgaagctcg ggacgggaca cctccgccgt tgcggtcaca
     1801 tggatgcgcc ccgccgtggt gcgcaacacc tgcgtccgcg gatcgggttg gaggcccatg
     1861 aagggcaatg cattccacag gatcaggttg gggccaaaga gccaggccgc tttcgagcca
     1921 tcgatcgccg agatgctgtc gggccacaga gcgatgtcgt cgtcgtcggt cgactgctgg
     1981 tactgggcgc tgcgagtgat cccctcggcg atcttccgcc acgggagtga gtcgtcgtac
     2041 tccgccaggc gatggacggc gcgggcatag cgtagtccgt tccactgcac gggccggccg
     2101 aaccagctcc acttgttcca gctcgcgccg aacaccggta tcgagccgta cagcaggaac
     2161 ggcttcgagt catcactcca caggtagaca aacgggagac cggcgcgcgc ccaacgcttg
     2221 gcatcggcga gccacgcctt atcgcccgag tacccgtacg ccgccacata ggcgtcgacg
     2281 gcatccgcgg cggccaggat gtccggcgcg tggacgggca cctcccatac ctgcgccgcg
     2341 cggggcacgc ggaactgccg catcagctcc agcggcctga gagccgcctg gtaaatggca
     2401 gggtcggccg tgagcagagc atgacgaatc agagtcgccg cccggttcgc gcacaggcca
     2461 agctccaacg cgttgtcggc gcccagctcg tggtagtcca tgcccacgaa ggggcccgtg
     2521 ccctcctggt cggcgtcgaa ccgccacccg ccgtcgggcc ccatccccgc gatgagtgac
     2581 gccgccgaag agtgcccact ggcgatctgc gcctcggccg cctcgccgaa ccgcagaccc
     2641 tcgtcctgcg cgagtgcgcg gattccggcg agtcccttca cgcggtcggc cagcgcccgg
     2701 cactgcgcgg ccagctcagc atcatccgtc agagccgcac caagcagcag atcgtacaag
     2761 tggttcggtg gatagccctt cgggcagagc acgggccccg ccccacggct cgaccaccag
     2821 atcgattcct cctcgatcca cagggatctg agatacgcct gcagcgagaa gtcgagttcg
     2881 gcggcgtatg agccacgcgg tatcgggtgc ggttccggga taccgtaccg ctggatccac
     2941 tcatccacgg cgaccagggc atcggtcgcc gcagcgcggg cgaggatgcc ggctgtgagg
     3001 cggagctccc cgcccgcctc cagctcgaac ggcgtgtccg ccagccgctg gttctccttc
     3061 acccacccgg cgcccgaagg cgcgaaaaga cccatgaggt gggactgctt gccctcgaag
     3121 tagtcaggcg acgcgaacac ggccgagggc cgatcgtgaa ctccatccca cttctgccgg
     3181 gcatcccaca gcaggcccac ggtcacgcca ttgtggtaca cgctcatcag agggatggtg
     3241 atcttgttgg gatgggggac gtagcggacc tgatccggat ggcccggctc gatgtccagc
     3301 gtgctcgacg accgctcgag cccctcaagc cactcgaggc ccgggaaaag cgcctcctgc
     3361 ttctgctcgc cgaaggcccc ctcgccggca tagagcatgg ggccctcgaa agccaggacc
     3421 cgggccgccc cgctgctctt ggccgaagcc gagaactcgg ccacgttcga ccccggcggc
     3481 accgtcaccg tccacgtcag atcccacgcg ctgcctccga cggacaagcc ggtccgggtg
     3541 aagaccaatc ggttctcctc ggcgcgcacc tggtccgccg tgaggaagac atcgatgggc
     3601 ccatcgtcac cctggacaac gagccggccc agtcgcggca tggccccgac gtaggcgaac
     3661 gcacccgtcc ccctcacata cagctcggca ggaccgtagc cgaacgacgc cttggcgaac
     3721 gccagtcgaa cgcggtcgtt gcccacaatg gccatgatgt cgttcgtgcc ggcccccacg
     3781 gcgaccgggg gcgggccggt gcagcgcgcc ccggagacca ggagcgtttc cagctcgccc
     3841 acaagctcgc ccccagcgcg gactgcggcg cgggcatggg ctcccggcgt ctcgcccgtg
     3901 gccgagaccg tccacgtgag agccaccctc tggccgggag cgaccgaagc cagcttccgc
     3961 gtggcgctcc cgcgaatggc aaagccctcc ggcacggaca gctcgcccgc cacatcgcga
     4021 gcgatggcgt gcccctcgtt ggtcagccag cagttcaccg cagtcgaggc gccgttccac
     4081 aagatgaacc gctgggtctg cagttgctcc agccgcagta cgggttcgca cacgagcgtc
     4141 gcctcgatgg gggccgcctg agtgccccca ctgacccgga tccgcgagcg gccctctcgg
     4201 cgcccggtga tccgccacac cacagtggcg tcgccacccg ggggcagagc gtcatgttcc
     4261 agaacactac ctcggcgcga cgcggacagg ttcttgggga gggccacctc gaagcggagt
     4321 ctcccggtag gctcatcgcc cacgtttcgg accgtgagcg taaggtcccc caccgtgccg
     4381 ggccgcatgg cgtcgtcgac cagctcgaac cgacgcagcc ggagcaccgg ccccaccttc
     4441 tcgacccact cgaagtcgtc gaggagtacg gtggccgcac cgcccaccag acggggcgat
     4501 atgtgaaccc acttcacggc ttcgttcgcg ctgaagtcat agggcaggcg cccctcgtgc
     4561 cacttcccgt ccccggcatg gtcatggggg atggtgaact cggcccgctg ttcgcccgta
     4621 tcctccagcg gcttggcccc catgggaatc gcgtaaacag acacgcgcgc cttcggcgcg
     4681 acggcctcca gcttgtacca gaagacgatc ccgcctgacc gccgatcgag catggcatct
     4741 cgccggcccg agtgggcctc ccatgcccgg ttcatccccg tctcgggtgc ggtctcgtcg
     4801 gcggttcgct cgagtcgcat ggctcgccgg cccgaatgag catcgtcgac cagcgccccc
     4861 gtgcccatga tctgccagtc caccggcagg ccatcgggcg tagccagctc gaacccgcca
     4921 ttggtgacgg ggttcggctc ctgaccgaac ccaatgccgc cgcatgccag gagagccagg
     4981 ccaatccggg ccgcggcgcg cacaacatgg tagcgcacaa ccaagtgatc gctcatcgga
     5041 gtctcacctc ggcgatcgaa tcacc
//
LOCUS       DAOSPL010000103         5064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_16668_length_5064_cov_2.905570, whole genome shotgun sequence.
ACCESSION   DAOSPL010000103 DAOSPL010000000
VERSION     DAOSPL010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16668_length_5064_cov_2.905570"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="PLQ54_02985"
     CDS             <1..937
                     /locus_tag="PLQ54_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOS92252.1"
                     /translation="LNATDAINTAIKGLVRPGDHVVSTLMEHNAVARPLKRLEAEGVE
                     VTYVPASAKGEVRAADVIGALRPETRLVAMIHASNVAGTLQPVEEVAAVTRCRDALLL
                     VDAAQTAGAVPVHIADMGIDLLAFAGHKGTLGPPGTGGLVLGMRADPRPLREGGTGTR
                     SESVEHPDELPERLEAGTMNVSGLVGLLEGVRHVMERSVEGIREHELAAAQAIEQGLA
                     RVPGVTLHGPRRFVRRAGVVSFSLENMDPRDVAAVLDTSFGIATRPGLHCAPLAHQSL
                     GTYPVGTVRMSPGPFTTEADVRAAVDAVAAIAGAV"
     gene            953..1585
                     /locus_tag="PLQ54_02990"
     CDS             953..1585
                     /locus_tag="PLQ54_02990"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOS92253.1"
                     /translation="MALSRIFAQFLAVGATAFGGALALIGLMQERLVERQRVVTSEEL
                     AEAVAVGQVLPGPVAVDAATHIGYRLRGLAGAVVATGGVVLPAFVIMLVLSPLYIAYG
                     KIPQAEMFFRGARAAVIAVVAAVCVRMGGKILKGKPQCVIAGLVFVLAVACRTGPGSQ
                     WVKPYVPVEAFLVAAAAVFGIVLCPPGADPAPRGGPAAASAEGRRDGEPS"
     gene            1585..2118
                     /locus_tag="PLQ54_02995"
     CDS             1585..2118
                     /locus_tag="PLQ54_02995"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOS92254.1"
                     /translation="MELLRLVGVCLKVGTLAFGGGMVMVPLMEPDVVGYGWVTHGEFM
                     DAVAMGQITPGPLLVTATYIGYKVAALPGAFLATIAMFLPSFLMTVACSRQLSRLQRS
                     PRVRSALQGIQAAVIGLMAAAAVILAYHGGLESWRGCVLALAALVAMVRFGVDASLVV
                     VGSGLLGFVLWWPGIGG"
     gene            2145..3296
                     /locus_tag="PLQ54_03000"
     CDS             2145..3296
                     /locus_tag="PLQ54_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS92255.1"
                     /translation="MIGVQITKKLRDFTLEVAFGLEDTGCVALIGPTGCGKSMTLRCI
                     AGLERPDVGRVEFAGRTLFDSANAVDEPPERRRVGFVFQDYALFPHMTVLENVSYGAR
                     ALGHSRSAAHRAARDAMALLGIGDLERQRPDRISGGQRQRTALARAVASQAEVLLLDE
                     PMAALDTRTRRRVRFELRSLFRRIRIPSVIVTHDPEDALTLGDLICVLHEGRILQAGT
                     RRELLWRPRSRFLAEFMGLNLLHGRARPISEHLSEVECGGQRFVATGRVDAPACLTVK
                     PSDVWIGREAPGHAGNRVEGTVAGLTYVGQAVRVTVEGAVALHAEVDLDEAEQMQLEP
                     GQTVWASFAPERATCYEETDGWLNSPSSKLRSTSARSSSRDSVWRHSGG"
     gene            3209..3952
                     /locus_tag="PLQ54_03005"
     CDS             3209..3952
                     /locus_tag="PLQ54_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HOS92256.1"
                     /translation="MAELTQLEIEKYKRQIQLPGFGVEAQRRLKSSAVLVTRLGGLGG
                     PTAFALAAAGVGKLVIAHGGVLTPSNLNRQILMRGDSVGQPRAPQAEETLRRFAPDLE
                     IVTYARDVSDDCVGEWVSQVDVACATTPDFREHYVLNRECVRQGKPLVHGGMNGMEGQ
                     LTTMVPGRTPCVECLVPEPPDWWDPAGFGVIGAVPGCLGNLMALEAIKVITGFGEPLA
                     GVMLTMDLEAGTFGRYSLTRRADCPVCGC"
     gene            3996..>5064
                     /locus_tag="PLQ54_03010"
     CDS             3996..>5064
                     /locus_tag="PLQ54_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOS92257.1"
                     /translation="MPHVLLALSSALAAPPPTYPGATWDLCEPAEVGLDAARLDAFRD
                     FAGGRGCVSRHGRLAYTWGDVSERADVASAAKPWYSYFLLRAIEDGRIGTLDDPIAPL
                     VPGLADLNPDLGHKDRGITWRHLATQTSCYGVTEAPGTAFDYSDWQMALFFDTLFVRA
                     YGAPWNTIDADVLHPLLTDVLQCEDAPTFLAFGPGDRPGRLSVSVRDFARFGLLFLRE
                     GRWRDRQLLSSEHARMLVSSPLPGDFPRTSGHAAAMLPGQRTMGSRTVPDDQCDHLGS
                     YSWLWWTNGTDGDGRRHWPDAPPDAYGALGHGGPRAMVVIPSLDLVASWNDARIDGRE
                     AENEALRLLVATVAGPAGQIVA"
BASE COUNT          716 a         1580 c         1889 g          879 t
ORIGIN      
        1 gctgaatgcc acggacgcga tcaacaccgc gatcaagggg ctcgtgcggc ccggggacca
       61 cgtggtatcg acgctcatgg agcacaacgc ggtggcgcgg ccgctcaagc ggctggaggc
      121 cgagggggtc gaggtaacct atgtgccggc gtcggccaag ggcgaagttc gcgccgccga
      181 tgtgatcggc gccttgcggc cggagacgcg cctggtggcc atgattcacg cctcgaatgt
      241 ggcgggcacc ctgcagccgg ttgaggaggt tgcggcggtg acgcgatgcc gcgatgccct
      301 gctcctggtc gacgcagccc agacggcagg agccgttccg gttcacatcg cagacatggg
      361 catcgacctg ttggccttcg ccggtcacaa gggcaccctg ggcccgccgg ggaccggggg
      421 cctcgtgctc gggatgcggg cggatccgcg gccactgcgc gaggggggaa cgggcacccg
      481 gtcggagtcg gtggagcatc cggacgagtt gcccgagcgg ctggaggccg gcaccatgaa
      541 cgtgtcgggc ttggtaggtc tccttgaagg tgttcgtcac gtgatggagc gcagcgtcga
      601 ggggatccgc gagcacgagc ttgcggcggc tcaggcgatc gagcagggtc tggcgcgggt
      661 tcctggcgtg acactccacg gtccacgccg gttcgtgcgc cgcgcgggcg tggtgagctt
      721 cagcctggag aacatggacc cacgcgacgt cgctgcggtg ctagacacgt cgtttggcat
      781 cgccactcgg cctgggctgc attgcgcgcc actggcccac cagtcgctgg gaacgtaccc
      841 tgtgggtacg gtccggatga gcccggggcc gttcacgaca gaggccgacg ttcgtgccgc
      901 cgtggacgcc gtggcggcca tcgcgggtgc cgtgtagaga ggggacaggg gagtggcgct
      961 gagtcggata ttcgcccagt tcctcgcagt cggagccacg gcgttcgggg gagcgttggc
     1021 gctcatcggc ctgatgcagg agcgactcgt cgagcggcag cgtgtggtta cgagtgagga
     1081 gctcgctgag gcggtggccg tcggccaggt gctgcccggc cctgtggccg tcgatgccgc
     1141 gacgcacatc ggctaccgat tgcgtggcct ggccggcgcc gtggtggcga ccggaggcgt
     1201 ggtgctcccg gcgttcgtga tcatgctggt gctttcgccc ctctacattg cctatggcaa
     1261 gatcccgcaa gccgagatgt tcttccgggg tgctcgcgcg gccgtgattg cggtcgtggc
     1321 tgccgtgtgt gtccgcatgg gtgggaagat actgaagggc aagcctcagt gtgtgatcgc
     1381 cggcctggtg ttcgtactgg cggttgcttg tcgcacgggg cccgggtcgc aatgggtgaa
     1441 gccctatgtg ccggtggagg cgttcctcgt ggcggccgct gcggttttcg ggatcgtgct
     1501 gtgcccgccg ggtgcggacc cggcgccccg tggcgggccc gccgcggcgt cggccgaggg
     1561 gcgacgggac ggggagccga gctgatggag ctccttcggc ttgtcggtgt gtgtctcaaa
     1621 gtgggcaccc tcgcctttgg cggcggcatg gtgatggtgc cgctgatgga gccggacgtg
     1681 gtgggatatg gctgggtgac tcacggcgag ttcatggatg ccgtagccat gggtcagatc
     1741 acgcccggtc cgctactggt cacggctacg tacattggct acaaggtggc ggcgctaccg
     1801 ggcgccttcc tcgccacgat cgccatgttc ctgccgtcgt ttctgatgac ggtggcctgc
     1861 tcgcggcagc tctcgcggct gcagcgcagc ccccgcgttc ggtcggcgct tcagggcatc
     1921 caggcggcag tcatcgggct gatggcggcg gcggccgtga tcctggccta tcacgggggc
     1981 ctggagagtt ggcgcggctg cgtgctggcg ttggcggctc tcgtggccat ggttcggttt
     2041 ggcgttgatg ccagtctggt cgtcgtgggc agcgggctgc tggggttcgt gctgtggtgg
     2101 cccggtatcg ggggctagag gacggccggg gcggagcggg tagtttgatc ggggtccaga
     2161 tcacgaagaa actgcgggac ttcacgctcg aagtggcgtt tggtctcgag gacacaggct
     2221 gcgtggccct catcgggccc acggggtgcg ggaagtccat gacgttgcgc tgcattgccg
     2281 ggctcgaacg gcccgacgtg gggcgggtgg agttcgcggg ccgcacgctg tttgacagcg
     2341 cgaatgcggt ggatgagccc cctgagcgcc ggcgcgtggg tttcgtgttc caggattacg
     2401 cgcttttccc ccacatgacg gtgctcgaga atgtgtcata cggggcacgg gcgctgggcc
     2461 acagccgttc ggcggcccac cgtgcggccc gggacgcgat ggccctactg gggattggag
     2521 atctcgagcg acagcggccg gaccggatct cgggcggaca acgtcaacga acagccctgg
     2581 ctcgggccgt ggcgagccag gcggaagtgc ttctgctcga cgagccaatg gccgcgttgg
     2641 acacccggac ccggcgccgg gtccggttcg agctgcggtc gctattccgg cgcatccgca
     2701 tcccttcggt gatcgtcacg catgacccag aggacgccct gacgttgggg gatctgattt
     2761 gcgtcctgca cgagggccgg atcctgcagg ccggcacccg ccgcgagttg ctgtggcggc
     2821 cgcggagccg tttcctggcc gagttcatgg ggctgaacct cctgcacggg cgagctcggc
     2881 cgatctcgga gcacctcagt gaggtcgagt gcggcgggca acggttcgtg gcgactggcc
     2941 gggtcgacgc cccggcttgt ctcaccgtca agccttcgga tgtgtggatc ggacgcgagg
     3001 cgcctggcca cgccggcaat cgtgtggagg ggaccgtggc cgggctcacg tacgtcggcc
     3061 aagccgtccg cgtgacggtc gagggggccg tggcgttgca cgccgaggtc gacctggacg
     3121 aggctgagca gatgcagctg gagccaggac aaaccgtgtg ggcatcgttt gcgcctgaac
     3181 gagccacttg ctatgaggag accgacggtt ggctgaactc acccagctcg aaattgagaa
     3241 gtacaagcgc cagatccagc tcccgggatt cggtgtggag gcacagcggc ggctgaaatc
     3301 cagcgccgtg ctggtcacgc ggcttggggg gcttggcggg ccgacggctt tcgcattagc
     3361 cgccgcgggc gtgggcaagc tcgtgatcgc acacggaggg gtgctgaccc cgtcgaacct
     3421 gaaccgccag atcctgatgc ggggcgacag cgtcggccag ccacgtgcgc cccaggcaga
     3481 ggagacactc cggcgattcg cgcccgacct ggagatcgtc acctacgcca gagatgtctc
     3541 ggacgactgc gtcggcgagt gggttagcca ggtcgatgtc gcgtgtgcaa cgacgcccga
     3601 cttcagggag cactacgtcc tgaaccgcga gtgcgtccgg caggggaaac cgctggtgca
     3661 cggcgggatg aacggcatgg agggccagct cacgacgatg gtgccgggcc gcactccctg
     3721 tgtggagtgt ctggtacccg aaccccccga ctggtgggac cccgcgggct ttggcgtcat
     3781 cggggccgtg cccgggtgcc tcggcaatct catggccctc gaggccatca aggtgatcac
     3841 ggggttcggc gagcccctgg ccggcgtgat gctgaccatg gatctggaag cgggcacatt
     3901 cgggcgctac tcgctaactc gccgtgcgga ctgccctgtc tgcggttgct gagcgaggtg
     3961 tccggtcggg gggagtcgcg gtgccgttgc tggccgtgcc gcatgtgctg ttggcgttgt
     4021 cttcggcgct cgcggcgcca ccgcccacgt acccgggcgc gacatgggac ctctgcgagc
     4081 cggccgaggt tgggctggat gccgcgaggc tggatgcctt ccgcgacttt gccggcggcc
     4141 gcggctgcgt gtcccgccac ggccggctcg cctacacgtg gggcgacgtc tcagagcgcg
     4201 ccgacgtcgc atcggccgca aagccgtggt atagctactt cctgctccgg gccattgaag
     4261 acggacgcat cggcaccctc gacgacccca tcgcaccact ggtgcccggg ctcgccgacc
     4321 tgaaccccga ccttggccac aaggatcgag gcatcacctg gcgccacctg gcgacccaaa
     4381 cgtcatgcta cggcgtcacg gaggcacccg gcaccgcctt cgattacagc gactggcaga
     4441 tggctttgtt cttcgacacg ctgttcgtcc gcgcctatgg ggcgccatgg aacaccatcg
     4501 acgcggacgt actacatccc ctgctgaccg atgtgctgca gtgcgaggac gcccccactt
     4561 tcctggcttt cggcccgggc gaccgccccg gccggctcag cgtatcggtc cgcgacttcg
     4621 cgcggttcgg actgctgttc ctgcgcgagg gacgttggcg tgaccgccag ctcctaagct
     4681 ccgagcacgc gcgaatgctg gtgtcgagtc cactacccgg cgacttccca cggacgtccg
     4741 gccacgcggc agcgatgctt cccgggcagc gcaccatggg ctcgcgcacg gtacccgatg
     4801 accagtgtga ccacctgggc agttacagct ggctgtggtg gacgaacgga actgacggcg
     4861 acggcaggcg gcactggccc gacgccccgc ccgatgcgta tggcgccttg gggcacggcg
     4921 ggccacgggc gatggtggtg atcccaagcc tggacctggt ggcaagctgg aatgacgctc
     4981 gcatcgatgg tcgcgaagcg gagaacgaag cgctgcgcct tctcgtcgcg acggttgcgg
     5041 gccccgctgg ccagatcgtg gcag
//
LOCUS       DAOSPL010000104         5057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_16695_length_5057_cov_3.519992, whole genome shotgun sequence.
ACCESSION   DAOSPL010000104 DAOSPL010000000
VERSION     DAOSPL010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16695_length_5057_cov_3.519992"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="PLQ54_03015"
     CDS             <1..964
                     /locus_tag="PLQ54_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92258.1"
                     /translation="RAFSPLGFEIDVWQVALGEDPRLSPPEPASVAGAPRLDEASGEY
                     VVRGTGYVVTVDGQTGAVRAIGLAGRPALTSMPSLMLLPANGDDVCGVQMSGVEPDIP
                     IFSDTCHNWRAKSVSARETAEGIEVRIVGAYDEAAGSFVLRFAQGSVIELGYEFTVTE
                     PGRCDPRQIGVVFDLPAGADTLSWRRRAEWSFYPNDHIGRPQGTARALVDPRTLCGLA
                     GPRSEPNWPWKDDANRYGTNDFRSTKRNILEASLARRDGGGLRVLSDGSQHVRAWVDA
                     GRVRLLVADYSNEGAAPFFSEHVVPRRPLEPGSRVQGTVRLELR"
     gene            1100..1312
                     /locus_tag="PLQ54_03020"
     CDS             1100..1312
                     /locus_tag="PLQ54_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92259.1"
                     /translation="MANWSRIMVAVDTLLEEIPGLQDEPHLEAYGDEAEDQLDGLASV
                     LLEARAADSAVCYLGRDRRHWWGRRH"
     gene            complement(1309..>1845)
                     /locus_tag="PLQ54_03025"
     CDS             complement(1309..>1845)
                     /locus_tag="PLQ54_03025"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOS92260.1"
                     /translation="GELARDPRVLAQFRPAEDNRVPNPGFEDGLADWDLRVEPAGAGS
                     CEAAAEDAHSGTRCLKVTSRGQGRAGVFSAFFPLELDARYEVSFWSKVVSHQGKGWPD
                     HGGLFAYRVRDDPEGRTGQAAFSNRAEGVWKQQRFVIHVTDPTWRFGRIMLNANEAKC
                     TTLFDDVVVRKVERAASP"
     assembly_gap    1846..1855
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1856..4317)
                     /locus_tag="PLQ54_03030"
     CDS             complement(<1856..4317)
                     /locus_tag="PLQ54_03030"
                     /inference="COORDINATES: protein motif:HMM:NF039329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92261.1"
                     /translation="MVVHLANAPGAGEPARSIPLTLENTSGVPWEGQTVGIGIPFARG
                     DLDSAEHIRVIDSGGHEVASQARQLSRWYPFGEAGIRWALVTFGPSVAAGQAPVYHVE
                     YGPGVARQVVPDPIRVDESVEAITVDTGPLRFVVSKRRASVIDQAWLEGQPIIAASDA
                     PSGSMITVLRHAGPTRYWSVGGERPVPSDVLTGSDGRPMSNPLANAKHDYRAVVEEHG
                     PLKVSIALHGCHADAHGRRFGPYTIRIHAFRGEARLGVTHQWVFSGVPDEERIQEASI
                     VLPLAFAPGRAICGADRPVALEGPAASLVQSAWNHCEVRDASGATIHMAEHGQGWLSV
                     DDGQRAVTAGLHDFWRDGPMSLACAGPRLTIGVYPSEAPPLDLKRFEGEVVDNPAPRT
                     GHGQSAQGLAKSREFELLFHAPGPEVATAFGARVQEPLWPRPDGRHVSNTGVLGHFWF
                     DASVYPRSSYGTQVLTRWTHYVRERLHPDDQLGLLNWGDIRGGWLPGMNDFIRPGVVP
                     QSYLELTQTHWGWSNSSLDAGFGLLIEYLRTGQRWQLREACAAARHMMDIDLVHYGDG
                     RGRYSMRPEDVGGIHRHGPQHWSNTAYGSYLQNYFATHLYGYYGLTGDETAREALLLS
                     GGNPLSMEAGTGDPLAVAYRFELAEDGEALHRLEAWAARLDSVGEFRWGFQDMANLAF
                     CQQLTGSETLQAALERNYQAACAKGAFDNFNGYCLRGLAQTWRLTGDELYRNALAARL
                     ADLLRGIRPKALPADPADPAIAEAMARLVETKVKHADLYAGYHQGYFTANVPFAASAL
                     EAAGITEDDVLSQKWAVTDEERG"
     gene            complement(4379..>5057)
                     /gene="ccsA"
                     /locus_tag="PLQ54_03035"
     CDS             complement(4379..>5057)
                     /gene="ccsA"
                     /locus_tag="PLQ54_03035"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOS92262.1"
                     /translation="AAAIALCIVAARHWVLTELRATLLLVGWAIPATYLIALRRAPIG
                     LATIAMTLAAAAVFGALAVPAQPLGQTVDHPWFFVVHVTAAVAGYAAFSLAFCCTVAY
                     LVQEALLKRKRLVGFSQRLPSLEAADAMGARLAALGFVLWTLMIATGALLAWLEVGEW
                     WSWTPKQTWSLLTWTAYAAYTHLRTIGDYRGRRTVLILAVGFVCAGLTYLLAGYLGDP
                     YHPFHSS"
BASE COUNT          821 a         1726 c         1749 g          751 t
ORIGIN      
        1 ccgggcattc agccctctcg gcttcgagat cgacgtgtgg caggtcgctc tcggcgagga
       61 cccgcggctc tcgccgcccg aaccggccag cgtagccggt gctccacgcc tcgacgaggc
      121 gagcggtgag tacgtggtcc gtggcacggg ctacgtcgtg acggtcgatg ggcagaccgg
      181 ggcggtgcgg gccataggtc tggcgggaag gccggccctg accagcatgc cgtcgctgat
      241 gcttctgcct gccaacggcg acgacgtctg cggtgtgcag atgtccggcg tggagccgga
      301 catcccgatc ttctccgaca cgtgccacaa ctggcgggcg aagtcagtca gcgcccgcga
      361 gacggccgag gggatcgagg tccgcatcgt gggtgcctac gacgaggcgg cgggttcctt
      421 cgtgttgcgg ttcgcgcagg gctccgtgat cgagctcggc tacgagttca cggtgaccga
      481 gccggggcga tgcgacccgc ggcagatcgg cgtggtgttc gacctccccg cgggcgccga
      541 cacgctctcg tggcgccgcc gggccgagtg gagcttctac cccaacgacc acatcggccg
      601 gccccagggg acagccaggg ccctcgtgga ccctcggaca ctgtgcgggc ttgccgggcc
      661 gcgctccgag ccgaactggc cgtggaagga cgacgccaac cgctacggga ccaacgactt
      721 ccggtcgacg aagcggaaca tcctcgaagc gtcactcgcc cgccgcgacg ggggtggctt
      781 gcgcgtgctc tctgacggga gccagcacgt cagggcgtgg gtcgacgcgg gacgtgtgcg
      841 actgctggtg gccgactact cgaacgaggg cgcggccccg ttcttcagcg agcatgtggt
      901 gccccggcgc cccctggaac ccgggagtcg cgttcagggg accgtgcggc tggagttgcg
      961 gtagaggcgt gggacgccag cagtctgtag cacctttagc cccataagac atgcaatgaa
     1021 atcttctaga ggccttgaca tgcatctgtc agcgtggtat aagaacatat gttcgcatac
     1081 cactgagagg gtgaacccca tggccaactg gagtcgcatc atggtcgctg tggacacact
     1141 gctcgaggag atcccagggc tgcaggatga gccgcatctc gaggcttacg gcgatgaggc
     1201 cgaggatcag ctcgacggtc tggccagtgt gctgctcgag gctcgggctg ccgactccgc
     1261 ggtctgctac cttggccggg accgccggca ctggtggggc cggcggcact agggcgaagc
     1321 ggcccgctcc accttccgga cgaccacatc gtcgaacagc gtcgtgcact tcgcctcgtt
     1381 cgcgttcaac atgatgcggc caaagcgcca tgtcgggtct gttacgtgga tcacgaaccg
     1441 ctgctgcttc cagacacctt cggcccggtt gctgaaagcc gcctgccccg tccggccctc
     1501 ggggtcatcc ctgactcggt aggcgaatag ccctccgtgg tcgggccacc ccttgccctg
     1561 gtgggacacg accttgctcc agaatgagac ctcgtagcgc gcgtccagct cgagcgggaa
     1621 gaaggccgag aacacgccgg cccggccctg gccacgcgag gtcaccttca ggcaccgcgt
     1681 gcccgagtgc gcatcctccg cggccgcctc acacgacccg gcgccggcgg gttccacccg
     1741 caggtcccag tcggcgaggc cgtcctcgaa ccccgggttc ggcacccggt tgtcctcggc
     1801 gggtcggaac tgggccagga ctcgcggatc gcgggccagt tcaccnnnnn nnnnnccacg
     1861 ctcctcatcg gtcacggccc acttctgcga gaggacgtca tcctccgtga tgccggccgc
     1921 ctccagcgcc gacgccgcga agggcacgtt ggcggtgaaa tagccctggt ggtagccggc
     1981 atagaggtcg gcgtgcttga ccttggtctc cacgaggcgc gccatggcct cggcgatggc
     2041 cggatcggcg ggatcggcgg gtagtgcctt cgggcgaatg ccgcgcagga ggtccgcgag
     2101 gcgagcggcc agggcgttgc ggtagagctc gtccccggtc agccgccaag tctgcgccaa
     2161 gccgcgcagg cagtagccgt tgaagttgtc gaaggcccct ttggcgcagg ccgcctggta
     2221 gttgcgctcc agcgccgctt ggagggtctc ggagcctgtc agttgctggc agaacgcgag
     2281 gttcgccatg tcctggaagc cccagcggaa ctcaccaacc gagtcgaggc gcgcggccca
     2341 ggcctccagg cggtgcagcg cctcgccatc ctcggccaac tcgaaccggt aggcgaccgc
     2401 cagggggtcg ccggtgcccg cctccatgct cagcgggttg cctccgctga ggaggagtgc
     2461 ctcgcgggcc gtctcatcgc cggtcagacc gtagtaccca tagagatggg tcgcgaagta
     2521 gttctgtagg tagctgccgt atgcggtgtt ggaccagtgc tgcggcccat gccggtggat
     2581 gccccccacg tcctcgggcc gcatcgagta ccgccctcgc ccgtcgccgt agtgcaccag
     2641 gtcgatgtcc atcatgtgcc gcgcggcagc gcacgcttcg cggagctgcc aacgctggcc
     2701 cgtgcgaaga tactcgatga gcaggccgaa cccggcgtcg agcgagctgt tcgaccagcc
     2761 ccagtgtgtc tgggtcagct cgagatagct ctgcggcacg actccgggcc ggatgaagtc
     2821 gttcatcccc ggcagccatc caccgcggat gtcgccccag ttcagcagcc cgagctggtc
     2881 atcgggatgc agccgctcac ggacgtagtg agtccatcgg gtcagtactt gggtgccgta
     2941 gctgctccgc ggatagacgc tggcgtcgaa ccagaagtgg cccagcaccc ccgtgttcga
     3001 cacatggcgg ccatcgggcc ggggccaaag cggctcctgc acccgagcgc cgaatgccgt
     3061 ggccacctcc gggcctgggg cgtggaacag gagctcgaac tcgcgggact tggccagccc
     3121 ctgagcgctc tgcccgtggc ccgtccgggg tgccgggttg tcgacgacct cgccctcgaa
     3181 gcgcttcaga tccagcggcg gcgcctcgct cgggtagacg ccgatagtca gacgtgggcc
     3241 ggcacacgcc aggctcatgg ggccatcgcg ccagaagtcg tgcagccccg ccgtcacggc
     3301 ccgttgaccg tcgtcgacgc tgagccagcc ctggccgtgc tcggccatgt gtatggtcgc
     3361 gcccgaggca tcgcggacct cgcagtgatt ccatgccgac tgcaccagcg aggccgccgg
     3421 gccttcgagc gccacgggcc gatcggcccc acaaatggcc cggcccggcg cgaaggccag
     3481 gggcagcacg atggaggcct cctggatgcg ctcctcgtcg ggaacgccgg agaagaccca
     3541 ctggtgggtt accccgaggc gagcctcgcc gcggaacgcg tggatgcgga tcgtatacgg
     3601 cccgaagcgc cgcccgtggg catcggcatg gcacccgtga agcgcgatgg agaccttcag
     3661 gggtccatgt tcctcgacca cggcgcggta gtcgtgcttc gcgttggcga gcgggttcga
     3721 catcggccgt ccatcggacc cggtcagcac gtcggatggc accggtcgct cgccaccgac
     3781 cgaccagtac cgggtggggc ccgcgtggcg cagcacggtg atcatggatc ccgacggtgc
     3841 atcggatgcg gcgatgatcg gctggccctc cagccaggcc tgatcaatga ctgacgcgcg
     3901 gcgcttcgac acgacgaacc tcagcggccc cgtatcgacg gtaatcgctt caacggactc
     3961 gtcgacgcgg atcgggtcgg gcaccacctg gcgcgcgacg ccggggccgt actcgacgtg
     4021 gtacacgggc gcctgaccgg ccgccaccga ggggccgaag gtcacgagtg cccagcggat
     4081 gccggcctcc ccgaacgggt accaccgcga gagctgccgg gcctgcgagg cgacctcatg
     4141 tccgccggag tcgatgaccc ggatatgctc cgcggagtcc agatcaccgc gggcgaaggg
     4201 gatgccgata ccgaccgtct ggccctccca tgggactccg gaggtgttct cgagcgtgag
     4261 aggaatggac ctcgccggct cgccggctcc tggcgcgttc gccagatgaa cgaccacggc
     4321 ggccgccaag cccaccaggc acacacagcc ggcgaccgcg gtacgacgcc ccgcagagct
     4381 acgaggagtg gaacgggtgg taggggtcac ccaggtagcc cgccaacagg tatgtgagtc
     4441 cggcgcagac aaagcccacc gccagaatga ggacggtcct ccgccctcgg tagtcgccaa
     4501 tggtccgcaa gtgggtgtag gcggcgtacg ccgtccacgt gaggagcgac cacgtctgct
     4561 tgggcgtcca actccaccac tcacccacct cgagccacgc caacagggcg ccggtggcga
     4621 tcatcagcgt ccacagcacg aagcccaggg cggccagacg agcccccatg gcgtcggccg
     4681 cctcgagcga cggcaggcgc tgggagaagc cgaccagccg tttgcgcttg agcagtgcct
     4741 cctgcacaag gtacgccacc gtgcaacaga aagccaggga gaacgcggca tagcccgcca
     4801 ccgccgcagt gacgtgtacg acgaagaacc aaggatgatc gacggtctgc cccaggggct
     4861 gcgcgggcac ggccaaagcg ccgaacacgg ccgcggcagc cagtgtcatg gcgatggtcg
     4921 ccagcccgat aggcgcccgg cgcaaggcaa tcaggtaggt ggccgggatg gcccatccca
     4981 ccaggagtag cgtggcgcgg agctcggtca gtacccagtg ccgcgccgcc acaatgcaga
     5041 gcgcgatcgc ggccgcg
//
LOCUS       DAOSPL010000105         5041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_16765_length_5041_cov_4.382872, whole genome shotgun sequence.
ACCESSION   DAOSPL010000105 DAOSPL010000000
VERSION     DAOSPL010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16765_length_5041_cov_4.382872"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="PLQ54_03040"
     CDS             <1..743
                     /locus_tag="PLQ54_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92263.1"
                     /translation="PLFHLSSITPNNTCLKSVPISPTNESPMAQQMFDSGDTAPVVRN
                     KLNQNAADVTAHHETQTNPHGTTLTQAHLAVTQTVSVPDGFFVRSLRKSGEPAGITDH
                     VTLSEGDGIDLTQVGQDITLVNTGISRVCLIPKLVISGVYGDGYLSIPCTDDRTAANF
                     RPVTGAAGFLSGAQTPYNGTSSGSHEYLHPQDHSGEGEPYDSFPMVWYDAVNGPVYEN
                     GQWRFIVQYRNVNNHLALWCRAERIGPE"
     gene            843..1103
                     /locus_tag="PLQ54_03045"
     CDS             843..1103
                     /locus_tag="PLQ54_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92264.1"
                     /translation="MDSPSWGDMIQLGSVGPILWFLYRMLPVLAKVGEAFTRLEQRIE
                     NHEQNQALLIHQHEEKMSALLSEQQRWCNYLLQRLNGHEPTS"
     gene            1212..1403
                     /locus_tag="PLQ54_03050"
     CDS             1212..1403
                     /locus_tag="PLQ54_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92265.1"
                     /translation="MPTITHATRLPDGGLRVVVTETRELLLPAHEAVRGGRLKPLAEL
                     KVLLAAQLAKQKGAEHARD"
     gene            1390..1590
                     /locus_tag="PLQ54_03055"
     CDS             1390..1590
                     /locus_tag="PLQ54_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92266.1"
                     /translation="MPAIELRDISRHETAWDAHFVVDGQHRHLKYRDAAPSPARALND
                     LEALLQAEADELALLALVGQEV"
     gene            1595..3316
                     /locus_tag="PLQ54_03060"
     CDS             1595..3316
                     /locus_tag="PLQ54_03060"
                     /inference="COORDINATES: protein motif:HMM:NF021665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G8 domain-containing protein"
                     /protein_id="HOS92267.1"
                     /translation="MASYTSTGSGNWSSPATWGGSGPPTNGDSVTVADTHTVVYDATI
                     NGAGLASLILNGVLSFAPATRTYLKMAGAITLGTSGELRIGTAANPIQAPSDAIPEMA
                     TLELQGNEITSANGTLSVHGECREGRDSVAQPQEPGDSSLTLVQGISLRAGDVIAVSQ
                     AAAGRLTSNATYTIQSYNAGTKMVTLTTTLGRAVAAGCSVILLSRNIAILGNNTSAKG
                     AFNGFANTPTLAGARFCDLAYGACPGRSGWTFEYCTFENCASYGAGRNAVRPTLNYCT
                     ALNGSLAHGIAPQVNHSTSHNSSSNMGGIFSTTSQGKAYATRTYNSTYGVLNDAGGQY
                     VDCVFAHCADGVQGASGTLFSGCTFTGNARDLRSLGGYQVVGCALDSSSLGAATEFSG
                     YNTTAVSGRNVYTESVNHDQVDGAFKAWMLGGVVTSVTDVKRPGRQRSYKHTLENANH
                     PVFSIWTHVVEPGQTLTISAWAYKTSSLAHLPRVWIIDPTRNPIADPTNCAPLAEDIM
                     ADVNGEWDALEVSWTHTDAGPVEVWFVTVAQGAAGDVYFDFAEAETAGPGGLPATSLL
                     GGVLQCA"
     gene            3307..4002
                     /locus_tag="PLQ54_03065"
     CDS             3307..4002
                     /locus_tag="PLQ54_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92268.1"
                     /translation="MRIAAGDVSRHLYFVAVDAADLKTRRTSLTHPAVWYSRDGGAKT
                     DVSSPLLEHLANGVFKLALDQAGMVTAAGDCCELVLHITADEMAPVTRVVEIVAQTEK
                     DHFDRLGVPAGESLAADVAAVNAVADAIQAKTANLPAQPAAVGSRMDLIDSPNATALG
                     SIADAVLDEEVQSAAAAGTSLRAAAKAAWAQGFGRWVLSGTTLTLYGPDGSTPVRAFT
                     LNDAENPTARTPA"
     gene            3999..4166
                     /locus_tag="PLQ54_03070"
     CDS             3999..4166
                     /locus_tag="PLQ54_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92269.1"
                     /translation="MKIILQGLTSSRLVTQGYHTAQWVAPSHTYPLEGTRNSRRLRAE
                     RIEGRLVARRS"
     gene            4171..4518
                     /locus_tag="PLQ54_03075"
     CDS             4171..4518
                     /locus_tag="PLQ54_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92270.1"
                     /translation="MSKSGRIERYRGDSYPETFTVTDAAGTPVNLEGAVVRFTVKDSP
                     HHTEAVLAKSSTQPGQIQLLTPGTFAVCFSPTEMSNLEVRRYLYDVEVTLGDGTIHTV
                     ACDYLDILADVTG"
     gene            4527..4892
                     /locus_tag="PLQ54_03080"
     CDS             4527..4892
                     /locus_tag="PLQ54_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HOS92271.1"
                     /translation="MKTPAVLTLAAAITLNAAANVLMKVSAMRLEARAAAGAPVGVVG
                     QYLEPVFLGGLVCFALALVAYRRSLQALPLNLAYPLMTSLGYLLVLGVSWLLLREVLS
                     PRQLLGCGVILAGVWLVAG"
BASE COUNT          900 a         1795 c         1547 g          799 t
ORIGIN      
        1 cccccctctt ccacctctcc tctatcactc cgaacaacac gtgcttgaaa tcggtgccca
       61 tttcccccac caacgagtct cccatggcac agcagatgtt cgacagcggc gacacggcgc
      121 ccgtggtccg caacaagctc aaccagaatg ccgccgatgt cactgcccac cacgaaaccc
      181 agacgaatcc ccacggtaca accctgaccc aggcgcatct ggccgtgacg caaaccgtga
      241 gcgtacccga tggcttcttc gtgcggagcc tacgcaaatc gggggagccg gccgggatca
      301 ccgatcacgt caccttgtcc gagggtgacg gtatcgacct gactcaagtg ggacaggata
      361 tcacccttgt caatacgggg atctccaggg tgtgcttgat cccgaaactg gtgatctcgg
      421 gcgtctacgg cgacggctac ctgagcatcc cctgcaccga cgaccgcacg gcagcgaact
      481 tccggccggt caccggcgcc gcaggcttcc tctccggcgc ccagacgccg tacaacggga
      541 cctcctccgg ctcccacgag tacctgcacc cgcaggacca cagcggtgag ggcgagccct
      601 acgacagctt ccccatggtc tggtacgacg ccgtgaacgg gccggtctac gagaacggcc
      661 aatggcggtt catcgtgcag taccgcaacg tcaacaatca cctggccctc tggtgtcgcg
      721 ccgagcgcat cgggccggag tagcgcagca cgcttacagc gggccggatg gcacccttcc
      781 cgcttcgcgg gccccttcca ttcggcccag agcccgccgt ttgccccaaa ggagctgatc
      841 ccatggattc cccttcctgg ggcgacatga tccaactcgg atccgtcggc cccatcctgt
      901 ggttcctcta ccgcatgctg cccgtactcg ccaaggtggg cgaagccttc acccggctcg
      961 agcagcgcat cgagaaccac gaacagaacc aggccctcct catccatcag cacgaggaga
     1021 agatgtcggc cctgctgtcg gaacagcaac gctggtgcaa ctacctcctc cagcggctga
     1081 acggtcacga gccgacatcg tgacaccctc acagcgggcc ggatggcacc cttcccgctt
     1141 cgcgggcccc ttccattcgg cccagagcca cgcagacacg gcacccctca tctacgcacc
     1201 aaggagcccc catgcccacc atcacccatg ccacccgact acccgacggc ggcctgcggg
     1261 ttgtcgtcac cgaaacgcgg gagttgctgc ttcccgccca tgaggccgtc cgaggtggcc
     1321 ggctcaagcc cctggccgag ctgaaggtcc tgctcgccgc ccagctcgcc aagcagaaag
     1381 gagccgagca tgcccgcgat tgagctccgg gacatctcgc gccacgagac cgcgtgggat
     1441 gcccacttcg tcgtcgacgg ccagcatcgt cacctgaagt accgcgatgc cgcaccgtct
     1501 cccgcccggg cactgaacga ccttgaggcc ctgctccagg ccgaggccga cgagttggcc
     1561 ctactcgcgc tcgtgggaca ggaggtgtag gcccatggcc agctacacat caacgggtag
     1621 cgggaactgg tcgagccccg ccacctgggg cggctccggc ccgcccacca atggcgacag
     1681 cgtgacggtc gccgataccc acaccgtggt ctacgatgcc accatcaacg gtgccggcct
     1741 cgccagtctg atcctgaacg gcgtgctgag cttcgccccc gccacacgca cctacctaaa
     1801 gatggccggg gccatcacct tggggacgtc gggcgagctc cgaatcggca cggccgccaa
     1861 ccccatccaa gccccttccg acgccatccc ggaaatggcg accctggagc tccagggcaa
     1921 cgagatcacc tcggccaacg gcacgctctc ggtacacggc gaatgccggg agggtcggga
     1981 ttcagtggcc cagccccagg agccgggcga tagctcactg accctcgtcc agggcatctc
     2041 cctgcgggcc ggagacgtga tcgccgtctc tcaggccgcg gcgggccggc tcacgtcgaa
     2101 tgccacctac acgatccaga gctacaacgc ggggaccaag atggtcacac tgaccacgac
     2161 cctggggcgg gccgtggccg cgggctgttc cgtcatcctg ctgagccgga acatcgccat
     2221 cctgggcaac aacacatccg ccaaaggggc attcaacgga ttcgccaaca cgcccactct
     2281 cgccggggcg cgcttctgcg atctggcata tggcgcatgc cccggccgct cggggtggac
     2341 gttcgagtac tgcacctttg agaactgcgc ctcctacggc gccggccgaa acgcggtgcg
     2401 gcctactctc aactactgca cggcgctcaa tggctcgcta gcccatggca tcgcgccgca
     2461 agtgaaccac agcaccagcc acaactcctc gagcaacatg ggcgggatct tctcaaccac
     2521 gtcccaaggc aaggcctatg ccacgcgcac ctacaacagc acctatgggg tcctcaacga
     2581 tgccggaggc cagtatgtcg actgcgtctt cgcccattgc gccgacggag tgcaaggtgc
     2641 cagcgggacc ctcttcagcg gatgtacgtt cacgggcaac gcccgcgacc tgcggtcgct
     2701 ggggggctac caggtggtgg gatgcgcgct cgatagctcc tcgctgggag ccgccaccga
     2761 gttcagcggc tacaacacca ccgcggtcag tggccgcaac gtctacaccg agagtgtgaa
     2821 ccacgaccag gtcgatggcg ccttcaaggc ctggatgctc ggcggcgtcg tgacttccgt
     2881 caccgatgtc aaacgtcccg gccggcagcg cagctacaag cacacgctcg agaacgccaa
     2941 ccacccggtg ttctcgatct ggacacacgt ggtcgagccc ggccagacgc tcaccatttc
     3001 cgcatgggcc tacaagacca gcagcctggc acatctgccc cgtgtctgga tcatcgaccc
     3061 gacccggaac cccatcgccg accccacgaa ctgcgccccg ctggccgaag acatcatggc
     3121 ggatgtcaac ggcgaatggg atgcgctgga ggtctcgtgg acccacaccg acgccgggcc
     3181 ggtcgaggtg tggttcgtga ccgtcgccca gggggcggca ggtgacgtgt acttcgactt
     3241 cgccgaggcc gagacggcgg gcccgggcgg cctgccggct acctcattgt tgggaggcgt
     3301 tctgcaatgc gcatagccgc cggtgatgtg agccggcatc tgtacttcgt cgccgtggac
     3361 gcggcggacc tgaagacgcg ccggacatcc ctgacccatc ccgccgtgtg gtactcgcgc
     3421 gacggcggcg ccaagaccga tgtgagtagc cccttgctgg aacacctggc gaacggtgtg
     3481 ttcaaactgg ccctcgatca agccggcatg gtcaccgcgg caggcgactg ctgcgagctg
     3541 gtgctacaca tcaccgccga cgagatggcc cccgtaaccc gcgtggtgga gatcgtcgcc
     3601 cagaccgaga aggaccactt cgaccgcctg ggagtgccgg cgggggagag ccttgccgcg
     3661 gatgtcgccg cggtgaacgc cgtggcggac gcgatccagg ccaagaccgc caacctgccc
     3721 gcccagccgg ccgctgtggg ctcccgaatg gacctgatcg acagtcccaa tgccacggca
     3781 ctgggttcca tcgccgatgc cgtgctcgat gaggaagtcc agtcggccgc agcggcggga
     3841 accagcctca gggcggccgc caaggcggcc tgggcccagg gcttcggtcg atgggtgctc
     3901 tcggggacca cgctcaccct ctatggcccc gacggatcca cccccgtccg cgccttcact
     3961 ctcaacgatg ccgagaaccc gacggcgagg acgcccgcat gaagatcatc ctccaaggcc
     4021 tgacgagttc gcggctggtg acccagggct atcacacggc gcaatgggtc gccccttccc
     4081 atacctatcc cctcgaaggc acgcggaact cccgtcggct ccgtgcggag cgtatcgagg
     4141 gacgtctcgt ggcgaggagg tcctgaggcc atgagcaaga gtggacgcat cgagcgctat
     4201 cgcggcgatt cgtaccccga gaccttcacg gttaccgatg ccgcgggcac ccccgtcaat
     4261 ctggagggag ccgtggtccg attcacggtc aaagactccc cccatcacac cgaagccgtc
     4321 ctggccaaga gctcgaccca gccggggcag atccaactcc tcacgcccgg aaccttcgcg
     4381 gtgtgcttct cgcccaccga aatgtcgaac ctcgaggtgc ggcgatacct gtacgatgtg
     4441 gaagtcaccc tcggggatgg cacgatccac acggtagctt gcgactatct ggacatactg
     4501 gcggatgtga cagggtaggg gagagaatga agacgccggc agtgctgacc ttggcggccg
     4561 cgatcacgct caatgccgcg gccaatgtgc tgatgaaggt cagcgccatg cgcttggagg
     4621 cccgcgccgc cgccggtgcg cccgtgggcg tggtggggca gtacctggag ccggtcttcc
     4681 tgggcggcct ggtctgcttc gccctggctt tggtcgccta ccggcgatcc ctgcaggcac
     4741 ttcccctcaa cctggcctat ccgctgatga ccagcttggg ctatctgctg gttctcggtg
     4801 tatcgtggct gctgctgcgc gaggtcctca gcccccggca gcttctcggc tgtggcgtca
     4861 tcctggccgg cgtgtggctg gtggcggggt gagggacggc gggccggatg gcgcccttcc
     4921 cgcttcgcgg gccccttcca ttcggcccct cctcgcgcga tcgcaggttt tcggaggggc
     4981 cgaatagggg cattggcggt aacttaggcc tgggcggtgc gcgggaggcc cgtacacggc
     5041 a
//
LOCUS       DAOSPL010000106         5029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_16813_length_5029_cov_2.854242, whole genome shotgun sequence.
ACCESSION   DAOSPL010000106 DAOSPL010000000
VERSION     DAOSPL010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16813_length_5029_cov_2.854242"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2658
                     /locus_tag="PLQ54_03085"
     CDS             <1..2658
                     /locus_tag="PLQ54_03085"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HOS92272.1"
                     /translation="SAVMFVRVMLLTAGLASFALPTLAVAAVPGMVVSLIAAAWLLVA
                     LALQTLYYTAFAAVYQRAFRTAGVRLGLSEMAPVVLASVFANLAAPHTGPMVFVEHAT
                     RKGHPLGRAVAGVVLAAAVDIATFALILAAGLAYLFSVHSLQPYEVAGAVGMGVTILA
                     WSTALVLGVTRPELLGRLLARLQRTANAVARRVRLPLAIADDWAARTAHEYGDAATVI
                     AQDRPGIVVTAAIAWVAHVIDLASLYAVAVAYGVALSPGGLVAAFSIGVLFWVVSVTP
                     QGVGLAEGSMALVLVSMGADADAAVAVALVFRGLSFWLPMGLGYAAVRRMPWARGPAR
                     AAAEPAGLQAIATLTAAMGVLNVVSAVTPSLRGRLLQLSEVMPLQVAYAGHLAAGLAG
                     FALLAVSGGLRRRKRAAWVIATTVLCASVASHLVKGLDYEEAALSTGLIVWLVAARRH
                     FHAASDPRTVRQALAIVAAAVAFTVFYGATGFYLHDRHYAVDYGLRDAIVQTLVMFSS
                     FRDPGIEPVTHFGRYFADSIYTVAAVTLGYGLLACLRPAVLRRPATPEERERARRIVE
                     AEGRSSLAPFALLPDKAYRFSPGGSVTAYTVKGRVAVALGDPIGPPRDARAAIADFVA
                     QCSLHDWVPAYYQTLPTYLDLYAAAGLRALCIGHEAIVRLSSFTLDGKPGRALRTPVN
                     RLTRLGYETRLHEPPLDDDLLAELRRVSDDWLALKHGREKGFSLGWFDEDYIGACPVL
                     AVHAPDGHIRAFANIISEYQLNEVTIDLMRHDAAAERGTMDLLFVRLMEWAKGRGYDG
                     FNLGLAPLAGIGDEPQDPLVEKALRFVYEHVTRFYNFRGLYDYKDKFHPEWSPRYLIY
                     PATEHLLAIGTAIVRASSGTSSLWAYLRR"
     gene            complement(2693..3042)
                     /gene="ssrA"
                     /locus_tag="PLQ54_03090"
     tmRNA           complement(2693..3042)
                     /gene="ssrA"
                     /locus_tag="PLQ54_03090"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(3054..3407)
                     /locus_tag="PLQ54_03095"
     CDS             complement(3054..3407)
                     /locus_tag="PLQ54_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92273.1"
                     /translation="MVIRARADGILTWLRLYGQQLLCTAHPEPPVVKPAEVQRSDRVA
                     DAVEAIRATGTPAWFEPTLGRAKAVAAQCPECRVYAPVGHLSLVPEPEPDLDDEEPCT
                     CRACHAAERPDLRVV"
     gene            3562..4956
                     /locus_tag="PLQ54_03100"
     CDS             3562..4956
                     /locus_tag="PLQ54_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92274.1"
                     /translation="MRVATTLVVCVWLASLAVAQETPAPQFGVEVNDRIDSTMAYTEI
                     GKNGVCSLWVIGGRRPRVLVSGDIPVAPRWVVRDRAFQWSPDGRRIAFASPRDNKFDI
                     YLAQPTGGAPSRVAHAFGPGKLAFDWDPTSRFLSIWVDDGRGGPVFVLDTAEHELAEV
                     SAGMVATRPVWATSQPKLAFVARSDKGRALYVVEAGAERAKASVCAEGGLSGNVWWSP
                     TGAELVAEAADADGTPRVLYMASASGGQVRPISQNVAQPRFADWSPDGRRVAFAGQVG
                     GTGLALYLATAPWAAPPVAIVGAPGDGMLLEQVGDAIGSEQWAAWSPSGDALCYVAGP
                     SDTVKNRWLYVAGPDGSGRRAMSEPVECRGFRWSPDGRSLLFAGRRGSQGRLSITAYV
                     VPASGSSADLRRLVPNVGHIAWSPDGRYVSYVTFDIYDRNHQLLQVADPTGAANPQRV
                     AEAMEEYAWAPVGR"
BASE COUNT          717 a         1733 c         1720 g          859 t
ORIGIN      
        1 tcggcagtga tgttcgtgcg ggtgatgctg ctgacggcag ggctggccag ctttgccctg
       61 ccgaccctgg ccgtggcggc cgtgccgggc atggtggtca gcctgatcgc cgcggcgtgg
      121 ctgctggtgg ccctcgccct ccagacgctc tactacacgg ctttcgccgc cgtgtaccag
      181 cgcgcgttcc gcacggcggg cgtcaggctc ggattgagcg agatggcccc ggtcgtcctc
      241 gcgtcggtgt tcgccaacct ggccgcgccc cacacgggcc ccatggtgtt cgtggagcat
      301 gcgacgcgga aaggccaccc gctgggtcgc gccgtggcgg gagtcgtcct cgcggccgcc
      361 gtcgacatcg ccaccttcgc cctgatcctg gcggcgggtc tcgcctacct gttctccgta
      421 cactcgctcc agccatacga ggtcgcggga gccgtgggga tgggtgtcac gatcctcgcg
      481 tggtcaacgg cgctcgtcct gggagtaacg cgacctgagc tcctcgggcg gctattggcg
      541 cgtctccagc gcacggccaa cgccgtcgcg cgccgagtga ggctgcccct ggcgatcgct
      601 gacgactggg cggcccgcac ggcgcatgag tatggagacg ccgcgaccgt catcgcgcag
      661 gaccgcccgg gcattgtggt cacggcagcc atcgcctggg tggcgcacgt gattgacctc
      721 gccagtctgt acgccgtggc cgtagcctac ggcgtcgcac tgagcccggg tggcttggtg
      781 gcggccttct ctatcggcgt gctcttttgg gtcgtctccg tcacccccca gggggtcgga
      841 ctggctgagg gcagcatggc tctcgtcctc gtgtcgatgg gcgcggacgc ggacgccgct
      901 gtggcggtcg ccctcgtgtt tcggggcctg tccttttggc tcccgatggg gctggggtac
      961 gccgctgttc ggcgcatgcc ctgggctcgg ggcccggcca gggccgcggc tgagccggcc
     1021 gggctacagg ccatcgctac actcacggcc gccatgggcg tgctcaacgt ggtgtcggca
     1081 gtgacaccct cgctccgcgg gcgcttgctt cagctctccg aagtgatgcc cctccaggtc
     1141 gcctatgccg gacacctcgc ggccgggctg gccggcttcg ccctcctggc cgtctcgggg
     1201 ggccttcgcc gtcgcaagcg cgcggcgtgg gtcatcgcga ccactgtgct ctgcgcctcc
     1261 gtggccagcc atctcgtcaa ggggctcgac tatgaggaag cggccctgtc aaccggcctc
     1321 attgtctggc tcgtggccgc gcgcaggcac ttccacgccg cctccgatcc gaggacggtt
     1381 cgccaggccc tggccatagt ggctgccgcc gtcgcgttca ccgtgttcta cggggcgacg
     1441 ggcttctatc tgcacgaccg ccactacgcc gtcgactacg gcctgcgtga cgccatcgtg
     1501 cagacgctgg tgatgttctc ctccttccgc gaccccggga tcgagccggt cacccatttc
     1561 ggccggtact tcgcggactc catttacacc gtggcagccg taacccttgg gtacgggctt
     1621 ctcgcctgcc tgcgccccgc cgtgctgcgc cgcccggcga cgcccgagga gcgcgaacga
     1681 gctcgccgca tcgtcgaggc cgagggccgg tcctcactgg cacccttcgc gctcctgccg
     1741 gacaaggcct accgcttctc gccgggaggc tcggtcacgg cctacaccgt caagggccgc
     1801 gtagccgtcg cactgggcga tcccatcggg ccgcctcgcg atgccagggc cgctatcgcc
     1861 gacttcgtgg cccaatgctc actgcacgac tgggtgcccg cctactacca gacactcccg
     1921 acctatctcg acctgtatgc ggccgcaggc ctccgcgcgc tctgcatcgg ccatgaggcc
     1981 atagtcaggc tgtccagctt cactctcgac ggcaagcccg ggcgcgcctt gcgcaccccc
     2041 gtgaaccggc tcacgaggct cggatacgag acccggctgc acgagccgcc ccttgacgac
     2101 gacctgctgg ccgagcttcg gcgcgtgagc gatgactggc tcgcgctcaa gcatggccga
     2161 gagaagggct tctcgctcgg gtggttcgac gaagactaca tcggcgcgtg cccggtgctc
     2221 gctgtccacg cacccgatgg ccacatccgc gcttttgcga acatcatcag cgagtaccaa
     2281 cttaacgagg tgaccatcga cctgatgcgg cacgacgccg cggccgagcg cggcaccatg
     2341 gacctcttgt tcgtgcgact gatggaatgg gcgaagggac gcggctacga cggcttcaac
     2401 ttgggtctgg cgcccttggc gggcatcgga gacgagccgc aggatccgct ggtggagaag
     2461 gccctccggt tcgtctacga gcacgtcacg cggttctaca acttcagggg gctgtacgac
     2521 tacaaggaca agttccaccc ggagtggagc cctcggtacc tgatctaccc ggccacggag
     2581 catcttctcg ctatcgggac ggccatcgtc cgcgccagtt ccggcacgag ctcgctgtgg
     2641 gcgtacctgc gcagatgaca acggcccgcc gggtgggcgg gccgtctgca catggtggag
     2701 gcggcgggag tcgaacccgc gtccgaggag ggaccagaag tagccactac gagcgtgtct
     2761 cgtgtttatg tctagctccg ggagccccca cgagcaggaa cccccatcgc gagttcacct
     2821 gcgatgtccc cgcgcgggcg atgaacggac ccggtagggt tagccgactt catgacgccg
     2881 tcttgacccg cgtcggcggg cggatcagac gacgctggct agctacgcag ccagaggaag
     2941 ctcagtgttg gcagttattg gttcccgcga gattaacgag cctgcgggga gctcggctcg
     3001 cgactacaac ccatccgcac cccgtcgaga ccagtcgccc cctttcacac ccattatacc
     3061 acccggaggt cgggacgttc agccgcatga cacgcgcggc aagtgcacgg ttcctcgtcg
     3121 tcgagatcag gctccggctc aggcaccagg ctcaagtgcc ctaccggagc gtagacccgg
     3181 cactccgggc actgagccgc gaccgccttc gcccggccga gtgtgggctc gaaccaggcc
     3241 ggcgtgcccg tggcccggat cgcctccacg gcgtcggcca cacgatccga gcgctgcacc
     3301 tcagcgggct tcaccacggg gggctccgga tgagccgtgc acagaagctg ctgcccgtag
     3361 aggcgcagcc aggttagaat gccgtcggca cgagcccgga tcaccatgat gggtactcgt
     3421 tcgccggtcg cgcggggccg tcctgcgtcg acccgtgccc gaggcacgga accacacccc
     3481 gctccggcgc atcctacgaa tgttggatca cccgggaatc ggcaccggtg ggctctcggg
     3541 cgagtttgag aggtgttgat catgagagta gcaacgactc tggtggtgtg cgtgtggctg
     3601 gcctcgctgg cagtggctca ggaaacgccg gcgccacagt tcggtgtcga ggtcaacgac
     3661 cgcatcgact ccaccatggc ctacacggag atcggaaaga acggtgtgtg ctcgctgtgg
     3721 gtcatcggcg gtcgcaggcc tcgggtgctc gtgagcggcg acatacccgt cgcccctcgg
     3781 tgggttgtga gagaccgcgc gttccagtgg tctccggacg ggagacggat cgccttcgcg
     3841 tcgccgcgag acaacaagtt cgacatctac ctcgcgcagc cgacgggtgg cgccccttcg
     3901 cgagtggccc acgccttcgg acccggcaag ctggccttcg actgggaccc gacatcgcgt
     3961 ttcctgtcga tctgggtcga cgacggccgg ggcggccccg tattcgtgct cgacacggcc
     4021 gagcacgagc ttgccgaggt gtcggcggga atggtggcaa cacggccggt ctgggccaca
     4081 tcccagccca aactggcatt tgtggcacgg agcgacaagg gccgcgccct gtatgtcgtc
     4141 gaggcaggag ccgagcgggc caaggcgtcg gtgtgcgccg agggcggcct ctccggcaac
     4201 gtctggtggt cgcccacggg ggccgagttg gtggccgagg cggccgacgc tgacggcacg
     4261 cctcgcgtcc tgtacatggc cagcgcctcg ggggggcagg tgaggcccat ctcgcagaac
     4321 gtcgctcagc cacgcttcgc ggactggtca cccgatgggc ggcgagtggc tttcgccggg
     4381 caggtcggcg gaaccgggct cgccctctac ctcgcgacgg ctccgtgggc cgcgccgccg
     4441 gtggccatcg tcggcgcacc cggtgacggc atgctgctgg agcaggtggg cgatgccatt
     4501 ggaagcgagc agtgggcggc ctggtcgccg tcaggcgacg cgctctgcta cgtcgccgga
     4561 ccatcggaca cggtcaagaa ccgctggctc tatgtggccg ggcccgacgg ctcgggtcgg
     4621 cgggcaatgt cagagcccgt cgagtgccgg ggcttccgat ggtcacccga cggccgttcg
     4681 ctgctgtttg ctggccgccg cggatcccag gggcggctct ccatcacggc ctacgtggtt
     4741 cccgcgtctg gctcgtcagc cgatctgcgc cgactggtgc ccaacgtggg acacattgcc
     4801 tggtcgcccg acggccggta cgtctcatat gtgacgtttg acatctacga ccgaaaccac
     4861 cagctactac aggtggctga tcccacaggc gcggccaacc cgcaacgcgt ggccgaagcc
     4921 atggaggagt atgcctgggc cccggtgggg cggtagggtc attggcggta gcttagggct
     4981 cccgtggccc atccgcctgg ttgcggaggg gcgccatggg gggaaccac
//
LOCUS       DAOSPL010000107         5025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_16828_length_5025_cov_3.428974, whole genome shotgun sequence.
ACCESSION   DAOSPL010000107 DAOSPL010000000
VERSION     DAOSPL010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16828_length_5025_cov_3.428974"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PLQ54_03105"
     CDS             complement(<1..412)
                     /locus_tag="PLQ54_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018306668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HOS92275.1"
                     /translation="MPSDPPGVDDRPQSRAYAAAGVDIDAAEETIRRFRAAVEATHGP
                     EVLGGIGHFGGLFAPDLSGLEEPVLVSGTDSVGTKIRLAFASGIHHTVGIDAVAMCVN
                     DVLTLGARPLFFLDCLSVGKVQPERDAQVVTGVAE"
     gene            complement(402..1859)
                     /gene="purF"
                     /locus_tag="PLQ54_03110"
     CDS             complement(402..1859)
                     /gene="purF"
                     /locus_tag="PLQ54_03110"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOS92276.1"
                     /translation="MDVRDPGAWRHRSDSPHEECGLIGVWTKDGADLARRCFFGLFTL
                     QHRGQESAGIAVTDGDGVACEKGMGLVTQVFTEDRIKSLRGHAGIGHTRYSTTGSTTI
                     QNAQPILGSFRGQPFALAHNGNLVNAVALRAEMEALGCRFVSTSDTELIVRLIELSPE
                     TEFEAALRGALTQVRGAYSLVILTPDRLIGLRDPWGIRPLCLGRDANDGYVMASETCA
                     LRLLQARLVREIEPGEMVVAGNDGLRAEVCEPYVRDSLCVFEFIYFARPDSRMYGREI
                     YGLRRKMGHMLAEQHPVDADLVIGIPDTGVPAAIGYSEASRIPYSEGLLKNRYIQRTF
                     IQPDPKMRRLGVSMKLAPLHDVLNGKRLVVVDDSIVRGTTTDQQVDLLRQAGAAEVHL
                     RICAPPIRYPCFYGIDMANQSELIAASLSVEQIRQKTGADSLGYLSIANLARALGIPR
                     DKFCMACFDGRYPLEIPQDVRATKFSLEKEAPDAQ"
     gene            complement(1890..2384)
                     /gene="purE"
                     /locus_tag="PLQ54_03115"
     CDS             complement(1890..2384)
                     /gene="purE"
                     /locus_tag="PLQ54_03115"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOS92277.1"
                     /translation="MKRVAIVMGSRSDLPVMEAAAETLRALGVPHSMTVASAHRTPER
                     VEELIAEYESSDVGVIIGAAGGAAHLAGAIAARTALPVIGVPVASGALNGLDALLSTV
                     QMPPGVPVAAVSIDGAVNAAVLAAQILASSDPEAMAAVRRYRAERSAAVVKSAEEVES
                     RYQG"
     gene            complement(2381..3652)
                     /gene="purD"
                     /locus_tag="PLQ54_03120"
     CDS             complement(2381..3652)
                     /gene="purD"
                     /locus_tag="PLQ54_03120"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOS92278.1"
                     /translation="MKVLVVGGGGREHALVWKIAQSPKVDRIYCAPGNAGIAACAELV
                     PLSAESIPDLMAFAQREAVDLTVVGPEAPLIAGIADEFAANGLRVFGPSRQAARLEGS
                     KVFCKNLFRRHRIPTADYQVFDCPKEAAKYVRARGVPIVIKADGLAKGKGVVVAHDLD
                     TALEAVDDMLVKKTFGSAGDRVVVEECLVGHELSLKVFTDGESIVPMEPARDYKRAHD
                     RDEGPNTGGMGAYSPVPLVSRDVANTAIRTVVRPIVDALRSEGIRYRGVLYAGLMLTN
                     SGLKALEVNCRFGDPETQVIIPRLGSDLVDVLLATCEDRLAEVVVDWSSRAAVCVVLA
                     SRGYPASAETGIPVHGLEAAGAMDDVVVFHAGTSRGTDGAIVTDGGRVLGVTAWGDSL
                     GAARKRAYEAASHVQFDGMQYRKDIAAGVTQ"
     gene            complement(3674..4102)
                     /locus_tag="PLQ54_03125"
     CDS             complement(3674..4102)
                     /locus_tag="PLQ54_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92279.1"
                     /translation="MSAFLGTYVVSFLLLGFGIVFVVGWAKAFRFRHPYVGFLGIALI
                     TFGVRQAFQFRPLVESALLGVAWVLLCWTAILMAAHSYANYRRAWAEYEKDRERRAAV
                     LMAEVQELARRARKPDRTEEPHRTESDTSQDSAPSYHSDP"
     gene            complement(4146..>5025)
                     /locus_tag="PLQ54_03130"
     CDS             complement(4146..>5025)
                     /locus_tag="PLQ54_03130"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOS92280.1"
                     /translation="ETQVQRDVAGLPTGFPDLDRIIGGLGRSTLTIVAARPSMGKTSL
                     ALNIATHVGLRPDDKRVVGVFSLEMSKEQLVEAALSSQAMINSHKLRLGDMRDEEWDK
                     LAQALDRLYDCNIYIDDTPGMTPLEMRAKARRLQAERGLSLLVVDYLQLVRYPLRTDS
                     RFEEVSAIARSLKAMARELEVPLIVVSQLSRSVERREDKRPILSDLMESGYIEAEADA
                     VVFIYRPEYYKYREAKTGQVVGGPAAQGAAEEPRDDTAEIIVAKNRTGPTGVCELVFR
                     GQYRRFDSKAGGRDGA"
BASE COUNT          852 a         1704 c         1654 g          815 t
ORIGIN      
        1 cttcagcaac accggtgacc acttgcgcgt cgcgctccgg ctggaccttc ccgaccgaca
       61 ggcagtcgag gaagaacaac ggccgcgccc cgagagtcag cacgtcgttg acgcacatgg
      121 ccacggcatc gatgcccacg gtgtggtgga tacccgaggc gaaggccagt cggatcttgg
      181 tgcccacgct gtcggtgccg gagaccagca ccggctcctc gaggccagac agatcgggcg
      241 cgaagaggcc accgaaatgc ccgatgcccc ccagcacttc gggaccgtgg gtggcctcga
      301 cggcagcccg aaagcgacgg atcgtctcct ccgcagcgtc aatatcgacc ccggctgccg
      361 cgtaggcccg gctctggggc cggtcatcca cgccgggtgg atcactgggc atcgggggcc
      421 tccttctcga gcgagaactt ggtcgcccgg acatcctgag ggatctccag cgggtagcgg
      481 ccgtcgaagc aggccatgca gaacttgtcg cgcgggatcc ccagggcacg ggccaggttc
      541 gcgatcgaca ggtagccgag cgagtccgct ccggtcttct gccgaatctg ctccacactc
      601 agcgaggcgg caatcagctc gctctggttg gccatgtcga tcccatagaa gcatgggtat
      661 cgaatgggcg gcgcgcagat ccgcagatgc acctcggcag cgccggcctg ccggagtagg
      721 tcgacctgct gatcggtggt ggtgccccgg acgatcgagt catccaccac caccagccgt
      781 ttgccattga gcacgtcgtg caggggagcg agcttcatcg agacacccag ccggcgcatt
      841 ttcgggtccg gctggatgaa ggtccgctgg atgtagcggt tcttgagtag cccctccgaa
      901 taagggatcc gcgacgcttc ggagtagccg atggccgccg ggaccccggt gtcggggatc
      961 ccgatgacca ggtcggcatc caccggatgc tgctccgcca gcatgtggcc catcttgcgg
     1021 cggaggccat agatctcgcg gccgtacatc cggctgtcgg ggcgtgcgaa gtagatgaac
     1081 tcgaacacgc acaatgagtc gcgtacatag ggctcgcaca cctcggcgcg cagaccgtcg
     1141 ttcccggcga cgaccatttc gccgggctcg atctcccgga cgaggcgcgc ctggaggagg
     1201 cgcaaggcac aggtctcgga ggccatcacg tagccgtcgt tggcgtcccg gccgaggcac
     1261 agcggccgga ttccccacgg atcccgcagt ccgatgagcc gatcgggtgt gagaatgacg
     1321 agcgaatagg ccccccggac ctgggtgagc gcgccgcgga gggccgcctc gaactcggtt
     1381 tccggcgaca gttcgataag gcggacgatc agctcggtgt ccgaggtcga gacgaaccgg
     1441 cagccaagcg cctccatctc ggcccgcagc gcgacggcgt tcaccaggtt gccgttgtgg
     1501 gccagcgcga agggctgacc gcggaagctc ccgaggatcg gctgggcgtt ctggatcgtc
     1561 gtggagccgg tggtcgagta ccgcgtgtgc ccgatacccg catggccgcg cagcgacttg
     1621 atgcggtcct cggtgaacac ctgggtgacg aggcccatgc ctttctcgca ggccactccg
     1681 tcgccgtccg tgacggcgat tcccgcgctc tcctgcccgc ggtgctgcag ggtgaacaag
     1741 ccgaagaagc accgccgggc cagatcggcg ccgtccttcg tccatacacc gatgagccca
     1801 cactcttcgt gtgggctgtc ggaccggtgg cgccaagcac ccgggtctct cacgtccatg
     1861 tccctccgga gcggctggcg agcccgctgc tagccctggt agcgcgattc tacttcctcg
     1921 gcgctcttga cgacagccgc ggaacgctcc gcccggtaac ggcggacggc ggccatggcc
     1981 tctgggtcgg acgaggccag gatctgagcg gccaacacgg ctgcgttgac ggcgccgtcg
     2041 atgctgaccg cggccacggg aacgcccggt ggcatctgca cggttgacag gagcgcatcg
     2101 agcccgttga gtgcgccaga ggccaccggt acgccgatga ccggaagggc ggtgcgggcc
     2161 gcgatggctc cggcaagatg ggcggcgccg ccggcggctc cgatgatcac gcccacgtcg
     2221 ctggactcgt actccgcgat gagttcctca acgcgctcag gcgtgcggtg tgccgaagcc
     2281 acggtcatgc tgtgcggcac accgagcgcg cgcaatgttt ccgccgccgc ctccatcacg
     2341 ggcaggtcgg accggcttcc catcacaatg gccactcgct tcactgggtc acccccgctg
     2401 cgatgtcctt ccgatactgc atcccgtcaa actgcacgtg actggcagcc tcgtaggccc
     2461 gtttgcgggc ggcccccagg ctgtcgcccc acgcagtcac gcccaataca cggccgccgt
     2521 cggtgacgat ggcgccatcg gtgcctcgcg atgttccagc gtgaaacacg accacatcgt
     2581 ccatggctcc ggccgcctcg agcccgtgga cggggatgcc tgtctcggcc gaagcagggt
     2641 agccacggga ggccagaacc acgcacacgg ccgcccgaga gctccagtcg acaacgacct
     2701 cggccaggcg gtcctcgcag gtggccagaa gcacgtcgac caagtcgctt cccaaccgag
     2761 ggatgataac ctgtgtctcc ggatcgccga agcggcagtt gacctcgagg gccttcaagc
     2821 ccgagttggt gagcatgagc cccgcataga ggaccccgcg gtaacggatc ccctcactgc
     2881 ggagggcatc gacgatggga cgaaccacgg tccggatcgc ggtattggcc acatcgcggc
     2941 tgaccagggg gacgggtgag taggcgccca tgccgccggt gttcgggcct tcgtcccggt
     3001 catgggcccg cttgtagtcc cgcgccggct ccatggggac gatggattcg ccgtcggtga
     3061 agaccttgag cgatagctcg tggccgacca ggcactcttc cacgaccacg cgatcgccgg
     3121 cggagccgaa ggtcttcttc acgagcatgt cgtcgacggc ctccagcgcg gtgtccaggt
     3181 cgtgggcgac caccacgccc ttgcccttgg ccaggccgtc ggccttgatg acgatgggaa
     3241 cgccccgggc tcggacgtac ttggccgctt ccttggggca gtcgaacacc tggtagtccg
     3301 ctgtcggtat gcggtggcga cggaacagat tcttgcagaa caccttgctg ccctcgagcc
     3361 gagccgcctg ccgcgacggc ccaaacacgc ggagcccatt cgccgcgaac tcgtccgcga
     3421 tgccggcaat gagcggcgct tcgggtccca ccacggtgag gtcaacggcc tccctctggg
     3481 cgaaggccat caagtccgga atggactcgg ccgagagagg aacaagctcg gcacacgctg
     3541 cgatgcctgc gtttcctggc gcgcagtaga tgcgatcgac cttcgggctc tgcgcgatct
     3601 tccagacgag cgcgtgctcc cggccgccgc cgccgacaac caggaccttc atccggcgtt
     3661 tcccccttag cgatcatgga tcactgtgat agctcggagc ggaatcctgc gaggtatcgg
     3721 actcggtgcg gtgtggttcc tcagtgcggt ccggcttccg cgcccggcga gcgagctcct
     3781 gcacctcggc catgagcacc gcggcccggc gttctcgatc cttctcatac tccgcccacg
     3841 cccggcggta gttggcgtac gagtgcgcag ccatgaggat ggcggtccag cacaacagca
     3901 cccaggccac acccaggaga gcggactcga cgagtgggcg gaactggaag gcctgccgga
     3961 caccgaaggt gatgagtgct atgcccagaa accccacgta gggatgacgg aaccggaacg
     4021 ccttggccca gcccactacg aacacgatgc cgaacccgag cagcaggaac gagaccacgt
     4081 aggtgccgag gaatgcgctc atgggccctc agctccgcac aagcccgggc ccattggcgg
     4141 cacggtcatg caccatcccg gcccccggcc ttgctgtcga accggcggta ttgcccccgg
     4201 aacaccagct cgcacactcc agtcggtccc gtgcggttct tggccacgat gatctccgcg
     4261 gtgtcgtcgc gaggttcctc cgcagcccct tgggctgccg gcccgcccac gacttgcccc
     4321 gtcttggcct cccggtactt gtagtactcg ggacggtaaa tgaacacgac cgcatcggcc
     4381 tcggcctcaa tgtagccgct ctccatgagg tcggacagga tcggccgctt gtcctcccgg
     4441 cgctcgacgc tgcgtgagag ctgtgagacc acgatcaggg gcacctccag ctctcgagcc
     4501 atcgctttga gcgagcgagc gatggcactc acttcctcga accggctgtc ggtgcggaga
     4561 ggataccgca cgagctgcag gtagtcgacg accagaagtg acaacccccg ctcggcctgc
     4621 agacgacggg ctttggcccg catctcgagc ggcgtcatgc cgggcgtgtc atcaatgtag
     4681 atattgcagt cgtagagcct gtcgagggcc tgggcgagct tatcccactc ctcgtcgcgc
     4741 atgtctccga ggcgcagttt gtgcgagttg atcatggcct gggaactgag tgctgcctcg
     4801 accagctgct ctttggacat ctcgagcgag aacacgccca ccacgcgctt gtcatcgggc
     4861 cgcaagccca catgggtcgc gatattgagg gcgagggagg tcttgcccat ggacggccgg
     4921 gcggccacga tggtgagggt agaccggccc agcccaccga tgatgcggtc caggtcgggg
     4981 aagccggtgg ggaggcccgc cacgtcgcgc tgaacttgcg tctcc
//
LOCUS       DAOSPL010000108         5022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_16844_length_5022_cov_3.020737, whole genome shotgun sequence.
ACCESSION   DAOSPL010000108 DAOSPL010000000
VERSION     DAOSPL010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16844_length_5022_cov_3.020737"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PLQ54_03135"
     CDS             <1..569
                     /locus_tag="PLQ54_03135"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HOS92281.1"
                     /translation="LLTETLRRSPGAVRSRHPTHSATVWGRDAAAIASGHPEATALGI
                     GSPFHRLAERGGKVLLLGVDCRRNSLIHVGEALSRPPYFGVVFYPGYHRDLLMVLPDG
                     REVVVPPRDNPGCSGGFNVVQDELASRGQLATGTVGDARSYLMLGEHVLQATRDLLAR
                     DAAALLCDDPGCAFCSAARECCDEAMRG"
     gene            complement(566..1435)
                     /gene="dapF"
                     /locus_tag="PLQ54_03140"
     CDS             complement(566..1435)
                     /gene="dapF"
                     /locus_tag="PLQ54_03140"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOS92282.1"
                     /translation="MDSAETLTALRFVKMHGTGNHFVLIDGRQGPERDWSELAEALCQ
                     ARYGVGADGLLVVLPSDRGVARMRMFNADGSEDMCGNGLRCVVRYLAGRGEMPAGHGI
                     VEALSGSREVAIVEREDQSWYRVELGEPSLAAADIPSLLPVDPAIDYALQVDGRPVRV
                     TLVSMGTPHAVVLNQMPPDDEEWRHLSCRIERHPAFPDRITATWAEVESSGSLRARFF
                     ERGVGPTLACGTGACAAVVAAALQGSCERSARVNMPGGSVDVEWRADGHVVLTGPAVT
                     VFEGEWPRVPCIP"
     gene            complement(1440..3557)
                     /locus_tag="PLQ54_03145"
     CDS             complement(1440..3557)
                     /locus_tag="PLQ54_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92283.1"
                     /translation="MGCDSSSGCCAGPRPFAVSASVRQALHRVAASGAPLLSFGQTVF
                     WDEPTKTLLIGAMESLGLQIPVVFGIHDTDYFSKLPRRGRAAAAGSSVCGMYSIAPHN
                     DGTTRELWAAVGEISALFASETPVSVRDLHHYRVPLRRLASGSPDGATSFIDRETEAW
                     GWRGLVRLDEHDIVGLDVAVDEANSRCIECMVRWGLETTLALVADEPTQAAVRAFTRQ
                     ALCHVRAALARHHGRRLSRAFRSILTRTIEAILGHVPTGLTVTACSEYFIFNTNTAQR
                     ARFAPIDWFLAPSTTEAARTAYNQAVAGSGAYTLAQFGDGALPFDVVVPNRGRGTLRL
                     AANEALVDLPGGTVSMGPTTFLRDRNDLAALVERRFGRNCMITAKAVVLPCLFLCEGV
                     MVLNEGASAYIGHGTRAFVQALRQAGVGFRLFPILRLHYGTFDSLGALSTEFTLPRHL
                     ARAFGKQRITAGEFASRWRQVVAEQEQLLGHVGWHSSPQAMVDHIRERDEATWGPVAE
                     EYRQVRQSMRAEGGRIRALAADLARARDDYRQARAHMIELERRSGCIRREILKPLWEA
                     GSNLTAEARTRLERAQEERSMLQAQIAATAAAARSASARWRALRAQIARLDREGPLAG
                     LRRRAQEIEHQVEMERFCIVRDALLTRGLVKADHRPSAWWMLALDGGRGWLPAVGESM
                     EAYLEELAPDVLPRCWSLAAGGQ"
     gene            3657..4574
                     /gene="murB"
                     /locus_tag="PLQ54_03150"
     CDS             3657..4574
                     /gene="murB"
                     /locus_tag="PLQ54_03150"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOS92284.1"
                     /translation="MHDPIATKLEELSATPIACNQPLAAYTSFRIGGPADYLASPTSA
                     DQVGSLVRAARDLSLPVTFMGQGTNLLVRDGGVRGLVIRLGPGLARIDVNPPRIRAET
                     GAKVRDLCEAAAEHSLTGLEFAVGIPGTLGGALAMNAGAYGGSIGDLVLEVRAVRTDG
                     ESRVFGRDDCAFGYRTSAFRAEGWIALEATLGLAPGDADAIRERHRDYCARRRASQPL
                     DAPSAGSVFKRPAGHFAGPLIESAGLKGTRIGGAQVSEKHANFIINTGGATAADVLAL
                     IDLVTARVLEVHGVRLEPEVIVIGEDHAA"
     gene            complement(4646..>5022)
                     /locus_tag="PLQ54_03155"
     CDS             complement(4646..>5022)
                     /locus_tag="PLQ54_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HOS92285.1"
                     /translation="VGDYSKDIAKVARRLHAEPYHWALEDIPLMGRRTHEMIRMALRS
                     FVERDLELARQVAEMDREVDSLWRTVRDQLIGHMRDDPGNVFQGTHLLLAARYLERIG
                     DHTVNVVERVSYMETGRLEHLV"
BASE COUNT          840 a         1749 c         1698 g          735 t
ORIGIN      
        1 gtctgctcac cgagacactt cggcgcagcc ccggggcggt tcggagccgc catccgaccc
       61 actcagccac ggtgtggggg cgcgacgccg ccgcgatcgc gtcagggcac cccgaagcaa
      121 cagctctggg tattgggagt ccgttccacc ggcttgccga gcgcggaggg aaagtgttgc
      181 ttttgggtgt ggactgccgt cgcaactcgc tcatccatgt gggcgaggcg ctgtcgcgtc
      241 ccccgtattt tggggtcgtg ttctaccccg gctaccaccg ggatctgctg atggtgttgc
      301 ccgacggacg tgaggtcgtg gttccacctc gcgacaaccc ggggtgcagc ggcggcttca
      361 acgtggtgca ggacgaactt gcctccagag ggcagctggc gacggggacc gtcggcgacg
      421 cccgaagcta tctgatgctc ggcgagcacg tgctccaggc cacccgggat cttctggccc
      481 gcgacgccgc ggcgctgttg tgcgatgatc caggatgcgc cttttgctcc gcggctcgcg
      541 agtgctgcga cgaggcgatg cgcggttagg ggatgcacgg cacccgcggc cactcgccct
      601 cgaacaccgt gacggccgga ccggtgagca cgacatgccc atcggcacgc cactcgacgt
      661 ctaccgagcc cccgggcatg ttcactcggg cggaccgttc gcacgagccc tggagtgccg
      721 ccgcaacaac cgcggcgcac gccccggtcc cacacgccag ggtcggaccg acaccgcgct
      781 cgaagaagcg ggctcgcagc gaacccgatg attcgacttc ggcccacgtg gccgtgatcc
      841 gatcggggaa ggcgggatgc cgttcgattc ggcaactcag atgccgccac tcctcatcat
      901 ctggcggcat ctggttcagc accacagcgt gcggcgtgcc catcgagacc agggtgaccc
      961 gcacgggtct gccatcgacc tgcaaagcgt aatcgatcgc cgggtcaact gggaggaggg
     1021 aggggatgtc cgcggcagcc aacgagggct caccgagctc aacacggtac caggactggt
     1081 cctccctctc gacgatggcc acttcgcgcg agccggagag cgcctccacg atcccatggc
     1141 cagcaggcat ctcgccgcgc ccggccagat agcgcaccac acagcggagc ccgttcccgc
     1201 acatgtcctc gctgccgtcg gcattgaaca tgcgcatgcg cgccacgccg cggtccgacg
     1261 ggagaacgac gaggaggcca tccgcgccga caccgtagcg agcttggcat agggcctcgg
     1321 ccagctccga ccagtcgcgc tcagggcctt ggcgcccgtc aatcaggacg aagtggttgc
     1381 ccgttccgtg catcttgacg aaacggagtg cggtgagtgt ctccgccgag tccacttggc
     1441 tactgccccc cggccgccag cgaccaacag cgggggagca cgtcgggagc cagctcctcc
     1501 aggtaggctt ccatggactc gcccacggcg ggcaaccagc cccggccgcc atcgagggcg
     1561 agcatccacc aagccgaagg ccggtggtcg gctttcacca gcccacgggt cagcagagcg
     1621 tcacgcacga tgcagaagcg ctccatttcc acctgatgct cgatctcctg agcccgccgc
     1681 cggaggccgg ccagggggcc ctcgcggtcc agacgagcga tctgtgccct cagggcgcgc
     1741 cagcgggccg acgccgaacg ggccgccgcg gcagtagccg cgatctgcgc ctgcagcatg
     1801 gatcgctcct cttgtgcccg ctcgagccga gtccgcgcct cggccgtgag gtttgacccg
     1861 gcctcccaca gcggcttgag gatctcccgg cggatacacc cgctgcgccg ttcgagctcg
     1921 atcatatggg cacgcgcctg gcgatagtcg tcacgcgccc gggccagatc ggcggccagc
     1981 gcccggatac gccccccctc ggccctcatg ctctgccgga cctggcggta ctcctcggcg
     2041 acaggacccc aggtggcctc gtcgcgctcg cggatgtggt cgaccatggc ctggggcgac
     2101 gaatgccagc ccacatgtcc gagcagctgc tcctgctcgg cgaccacttg gcgccagcgc
     2161 gaggcaaact cccccgccgt gatccgctgc ttgccgaacg cccgggccaa gtgacggggg
     2221 agcgtgaact ccgtcgacag ggcacccagg ctgtcgaagg tgccatagtg gagtcggagg
     2281 atggggaaca ggcggaagcc cacaccggcc tgcctgaggg cctggacgaa tgcacgcgtg
     2341 ccgtggccga tgtacgccga cgccccctca ttgagtacca tgacgccctc gcagaggaac
     2401 aggcacggca ggaccaccgc cttagccgta atcatgcagt tccttccgaa ccggcgctcg
     2461 acaagcgcgg ccaggtcgtt ccggtcccgc aggaaagtgg tcggccccat ggacacggtg
     2521 ccaccgggca gatcgaccag cgcctcgttg gcggccaggc ggagcgtccc gcgtccccga
     2581 tttgggacca cgacgtcgaa gggcaacgct ccgtctccga actgcgcaag cgtgtaggcg
     2641 cccgagcccg cgacggcctg gttgtaggcc gtgcgagccg cctcggtcgt gctgggagcc
     2701 aggaaccagt cgatgggcgc gaaacgagcc cgctgggcgg tgttggtgtt gaagatgaag
     2761 tactcgctgc acgcggtgac cgtcaggcca gtgggaacgt ggcccagaat ggcctcaatc
     2821 gtgcgcgtga ggatggaccg aaacgctcgg ctgaggcggc gcccatgatg tcgagccagg
     2881 gccgcccgaa cgtgacacag agcctggcgg gtgaacgcac gcacggccgc ctgggtcggc
     2941 tcgtccgcga ccagagcgag cgtggtctcg agcccccagc ggaccatgca ctcgatacac
     3001 cgcgagttgg cctcgtccac cgcgacatcg agccccacga tgtcgtgctc gtcgagccgc
     3061 acgagaccgc gccagcccca cgcctcggtc tcgcggtcga tgaaggaggt tgccccgtcg
     3121 ggagatcccg acgccagacg tctcaggggc acgcggtagt ggtgcaggtc gcgcacggag
     3181 accggggtct cgctggcgaa cagggcagag atctcgccga ccgcggccca cagctcgcgg
     3241 gtcgtgccat cgttgtgcgg ggcgatgctg tacatcccac acacggagct accggcggca
     3301 gccgcccggc cccgccgcgg caacttgctg aagtagtcgg tgtcgtggat gccaaagaca
     3361 accgggatct gcaggcccag ggactccatg gcgccgatca gcagagtctt ggtcggctca
     3421 tcccagaaga cggtctggcc aaacgagagc agaggggctc cggaggccgc cacccggtgc
     3481 agtgcctggc gcaccgaagc gctgacggca aatggccggg gacccgcgca acaacctgaa
     3541 ctggagtcgc atcccatggg gacccttccg ttcgtcgccc cagcgagtaa gccattatac
     3601 tgtatgaact cagccgacgg ccggcgggcc gagccaggca gaccaggacg gtaaccgtgc
     3661 acgaccccat cgccaccaag ctcgaggagc tctccgccac acctatcgcg tgtaaccaac
     3721 cgcttgcggc atacacttcg ttccgcatcg gcggcccggc cgactatctg gcatcgccca
     3781 cgtcggcgga tcaggtcggc tcactcgtgc gcgcggcgcg cgacctatcg ctaccggtga
     3841 ccttcatggg ccagggcacg aacctgctgg tccgagacgg cggcgtccgc ggtctggtga
     3901 tccgcctggg tccaggcctg gcacgcatcg acgtgaatcc gccgaggatt cgcgccgaga
     3961 caggggccaa ggtgcgcgac ctgtgcgagg ccgcagccga gcatagcctg acgggtctgg
     4021 agtttgccgt cggcatcccc ggcacgctgg ggggcgctct ggccatgaac gcgggggcct
     4081 atgggggatc catcggcgac ctcgtgctcg aggtgcgggc ggttcgcacc gacggcgaga
     4141 gccgggtgtt cgggcgggac gactgcgcct tcggctaccg gacgtcggcc ttccgcgccg
     4201 aaggctggat cgcgctggag gcgacccttg gactggcgcc cggcgatgcc gacgccattc
     4261 gcgagcgcca ccgcgactac tgcgcgcgac gacgcgcgag ccagccgctg gatgccccaa
     4321 gcgccgggag cgtgttcaaa cgcccggcgg ggcacttcgc cgggccgctc atcgagtcag
     4381 cgggcctcaa aggcacccgg atcggcggag cccaggtgtc ggagaagcac gccaacttca
     4441 tcatcaacac cggcggggcc accgcagccg acgtgctggc tctgatcgac ctcgtcacgg
     4501 cccgagtcct cgaggtgcac ggcgtgcgcc tcgaacccga ggtcatcgtc atcggcgagg
     4561 accacgcggc ttaggaacgc aagacggtcg ccgcgggcac cgcgccggct gctcccatcc
     4621 aagcaagggg accggcgccc tcggcctaca cgaggtgttc gaggcgaccg gtctccatgt
     4681 agctgacgcg ctcgacaacg ttcaccgtgt gatcgccaat gcgctcgaga taacgggccg
     4741 cgagcaacag gtgggtgccc tgaaagacgt tgcccgggtc gtcgcgcatg tggccgatga
     4801 gttggtccct cacggtgcgc cacagggaat cgacctcacg atccatctcc gcgacttgcc
     4861 gcgccagctc gaggtcccgc tccacgaacg aacgcagtgc catccgaatc atctcatggg
     4921 tgcgcctgcc catgagcggg atgtcctcga gggcccagtg atagggctcg gcgtggaggc
     4981 gccgggcgac cttggcgatg tccttagagt agtcgcccac gc
//
LOCUS       DAOSPL010000109         5015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_16873_length_5015_cov_3.258871, whole genome shotgun sequence.
ACCESSION   DAOSPL010000109 DAOSPL010000000
VERSION     DAOSPL010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16873_length_5015_cov_3.258871"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3298)
                     /locus_tag="PLQ54_03160"
     CDS             complement(<1..3298)
                     /locus_tag="PLQ54_03160"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOS92286.1"
                     /translation="MNGALCGALSVSMGLVLWPGAEGRAAAEGPADDYLSGGLFDRIE
                     AYTLAWGELGIDTAVRPTDGRAPQPLRIGDRTFTRGLGHHASGEIVVDLSGEFETFEA
                     DVGVQWQDGGTGTVVFRVLADGVEIYDSGTMTERDPARHVAVSVAGVDELELVVTDAG
                     DGITCDCANWANARLVRGTQPSRAAAERFDAAPFGEVVKSDPARVAGTQASRVEEFPA
                     ADVYLTQEILPSRDGLYAVPQWPDGRGAIGLQWAERRFVREVGICLPREARTMAGKVR
                     AQYWAGESLWQGQWQPLDGETVFEGPWLVTRAFWRGDPETRRRGTQKIRWVFDADALP
                     AEVSGFRARTRSRWREVSLEFRAAPAGPNAGGRVEIHNGWAIAAAEPVRVVNWDMAQC
                     LRVTVRATRPRSSRSDATVLRLTCPDGAFALAVDDVLEEGWAYVPAFGVLVADATRAP
                     SLDAVRSQLSRVSSYLDQVRARPDQTFQQAMAHVHRDVQDHGPTMLSLACDDCKFVAD
                     EDGTLGRYREGQVHPSYEIRGTPQFGSTAAKRQGRRLYRGWYPVPEITFADGPVTYTQ
                     RAFVAPFGAPPTDTRVGRHRSIGMAQVVLANESGASAPVRLALEFAADSEPRLALDRL
                     AGGDGVVALQGDRLVALFQRESGPELSVMTNGTRVVVSGELPARSSAEWTLALPGWTV
                     GAGSRAFSGRAQERLADTMAYWDSVLGSAMAVELPDELLTNVIRASQVHCLMAARNEG
                     DGARISPWISSDRYGPLESEANSIVLGMDQFGHHAFSRASLDYFIARYNPAGFLTTGY
                     TLMGTGWHLWVLGEHHSLTGDDDWLRSHADDVARVCRWIVAQRAKTEGVDAHGERTPE
                     SGLVPPGVVADWGMYAYRFCMEAHYCAGLRDAARALTAIGHPDGPAFAAEAEAFRDDL
                     VRAYRWAQARTPAMRVSDGTFTPGVPSMVHCFGKTGEFFAGEDWNRSWAGDVEIGPHH
                     LAALGLMDPASRDVDGMVATLEDYWFLQDGMGAYPGEASRADWYHLGGFSKVQPYYTR
                     VAEIHALRDDVKPFIRAYFNAIPSLLNCENLSFWEHFHNTGGWNKTHETGWFLTQTRT
                     MLVTERGSELW"
     gene            3544..4119
                     /locus_tag="PLQ54_03165"
     CDS             3544..4119
                     /locus_tag="PLQ54_03165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS92287.1"
                     /translation="MDPARQERIARELRELADKNYRAIYHLIHSYIGGDPDEAEDLTL
                     QTFENAFASYHRFRGEADVRTWVFRIAINVCKNRIRQKQTRRRFEALSLDATFGGDSE
                     DDTSAMDVPDVSQSPTRIAEGRELREILRAGIRKLPADYREVLLLKLQDFSYKEIADM
                     LDCTVETVKSRLFRSRVALKQLVGGYVDEAD"
     gene            4271..>5015
                     /locus_tag="PLQ54_03170"
     CDS             4271..>5015
                     /locus_tag="PLQ54_03170"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOS92288.1"
                     /translation="MRVGAERGTAEPQPGALTLRWLMLSALLAAVLTSVADAQKRPIV
                     REIRIDIGPHGQISEEVVRSELTAVSVGMEYDQAKTAAALRAVLDTGYFQRDPKLSYV
                     EAVPQGDDVALVFRLRENPIIESVTVEGNTVLDDATITALLREVLQPGRVLNSRMVDE
                     WIQEAVSQAYRDRGYAAVVLSPTLSEQGVLTIAVVEQYIEDIRLAGLRKTAEKVVRRE
                     IEARPGQLVNTHRIQEDLDRLLALGIFEDV"
BASE COUNT          851 a         1751 c         1659 g          754 t
ORIGIN      
        1 tccacagctc gctcccacgc tccgtgacga gcatggtacg cgtctgggtg aggaaccacc
       61 cggtctcatg ggtcttgttc catccgcccg tgttgtggaa gtgctcccag aacgacaggt
      121 tctcgcagtt gagaaggctg ggaatggcgt tgaagtaggc gcggatgaag ggcttcacat
      181 cgtcacgcag ggcatggatc tccgccaccc gcgtgtaata gggctggacc ttcgagaaac
      241 cgcccaggtg gtaccagtcg gcgcggctgg cctcgcccgg ataggctccc atgccgtcct
      301 ggaggaacca gtagtcctcg agggtggcca ccatgccgtc gacatcgcgg ctggccggat
      361 ccatgaggcc gagggcagcc agatggtgcg gcccgatctc gacgtcgccg gcccaggaac
      421 ggttccagtc ctcaccggcg aagaactcgc ccgtcttgcc aaagcagtga accatcgacg
      481 ggacgcccgg cgtgaatgtg ccgtcagaca cgcgcatcgc cggggtccgc gcctgcgccc
      541 agcgatacgc ccggaccagg tcgtcgcgga aggcctctgc ctcggcggcg aaggccgggc
      601 cgtctgggtg gccgatggcc gtaagcgctc gagccgcgtc ccgcagaccg gcgcagtagt
      661 gggcctccat gcagaaccgg taggcgtaca tcccccagtc agccacgacg ccggggggaa
      721 cgaggcccga ttcgggcgtc cgctcaccat gggcgtcaac accctcggtc ttggcccgct
      781 gcgccacgat ccatcggcac acgcgtgcca cgtcatcggc atgggagcgc agccagtcgt
      841 cgtcccccgt cagcgagtgg tgctcgccca ggacccatag gtgccatccg gtgcccatca
      901 gcgtgtagcc cgtggtgagg aagcccgccg ggttgtaccg cgcgatgaaa tagtcaagcg
      961 atgcccggga gaacgcgtgg tgcccgaact ggtccatccc caggacgatg gagttggcct
     1021 cgctctccag cggcccatac cggtcggaag agatccacgg cgagatccgc gcgccgtcgc
     1081 cctcattccg cgccgccatg aggcaatgca cctgtgaggc gcggatcaca ttcgtcagca
     1141 actcgtcggg gagttcgacc gccatggccg agcccagaac gctgtcccag tacgccatcg
     1201 tatccgcgag ccgctcctga gcgcgcccag agaaggcccg gctgccggcc ccgacggtcc
     1261 accccggcag ggccagtgtc cactccgccg agctgcgcgc gggaagctcc ccggacacga
     1321 ccacgcgggt cccgttggtc atgaccgaca gctccgggcc gctctctcgc tggaacagcg
     1381 cgaccagacg gtctccctgg agggcgacca cgccatcgcc gccggccagc cggtcgagcg
     1441 cgagcctcgg ctcggagtcg gcggcgaact cgagggccag gcgcacggga gcgctggcgc
     1501 ccgactcgtt cgccagaaca acctgggcca tgccgatcga ccggtgccga cccacccgcg
     1561 tatcggtcgg cggcgcccca aatggcgcca cgaacgcgcg ctgcgtgtag gtaaccggcc
     1621 cgtccgcgaa cgtgatctcg ggaaccgggt accatccgcg gtacagccgc cggccctgac
     1681 gtttcgcggc agtcgagccg aactgcggtg tcccgcggat ctcgtaggac gggtgcactt
     1741 gcccctcgcg ataccggccc agcgtgccat cctcgtcggc cacgaacttg cagtcgtcgc
     1801 acgcaagcga cagcatggtg gggccgtggt cctgcacatc ccgatgaacg tgggccatgg
     1861 cctgctggaa ggtctggtcg ggccgagcgc gcacctgatc cagatagctc gatacccggg
     1921 acagctgcga gcgcactgcg tccagggagg gcgctcgagt ggcgtcggcc accagcacgc
     1981 cgaacgccgg gacgtaggcc cacccctcct cgagcacatc gtcaaccgcg agggcgaatg
     2041 cgccatctgg gcaggtcagg cggagcacag tggcatccga acggctgctc cgagggcgcg
     2101 tggcacgcac cgtcacgcgc aggcactgcg ccatgtccca gttaaccaca cggaccggct
     2161 cggccgccgc gatagcccaa ccgttgtgga tctcgacccg gccgccggcg ttcggtccag
     2221 ccggagccgc ccgaaactcg agcgacacct cgcgccagcg cgagcgagtc ctggcgcgga
     2281 accccgagac ctcggcgggc agagcgtcgg catcgaagac ccagcggatc ttctgggtgc
     2341 cgcgtcgccg cgtctcgggg tcgccgcgcc aaaaggctcg ggtgaccagc catggcccct
     2401 caaagaccgt ctccccgtcc aggggctgcc attggccctg ccagagcgac tcacccgccc
     2461 agtactgggc ccggaccttc ccggccatgg tccgcgcctc tctgggtaga cagatgccca
     2521 cctcgcgcac gaagcggcgc tcggcccact gcagcccgat ggcaccccgc ccgtccggcc
     2581 actgtgggac ggcgtacagc ccgtcacggc tgggcaggat ctcctgcgtc aggtagacat
     2641 cggccgccgg gaactcctcg acccgactgg cttgcgtgcc ggccacccga gccgggtcgg
     2701 acttcaccac ctcgccgaag ggagccgcat caaaccgctc ggcagcagca cggctcggtt
     2761 gcgttccgcg cacgagccgg gcgttcgccc agttggcgca atcgcaggtg atgccgtctc
     2821 cggcatcggt caccaccagc tccagctcgt cgacacccgc gacagagacc gcgacatggc
     2881 gcgcggggtc tcgctcggtc atggtcccgc tatcgtagat ctcgacccca tcggcaagca
     2941 cgcggaagac gacggtgcct gtcccgccgt cctgccactg cacaccgaca tcggcctcga
     3001 aggtctcgaa ctccccggag aggtcgacga cgatctctcc ggatgcgtga tggccgaggc
     3061 cgcgggtgaa cgtgcgatcg ccgatccgaa gcggctgagg ggcgcggccg tctgtgggcc
     3121 gcaccgcggt gtcgatgccc agctcgcccc aggcgagcgt gtacgcttcg atgcggtcga
     3181 acagcccgcc gctcagataa tcgtccgccg gcccctctgc ggcagcgcgg ccctcggctc
     3241 ctggccagag gaccagcccc atgctcacac tgagggcgcc acagagtgcg ccattcacct
     3301 gcaaatccca ccgcctccag cgtccaacct agatgagtgg ccattccgcc gggcgaacgc
     3361 gcctccctgc ccgtcgccca cccagaacgt gaacactgca ttaccggcct ggcaactccg
     3421 gacggaaatg gggtagtatc ccgtgggcgt ttgcggactg cgtcaggccg ccgcagggaa
     3481 ggagggattg gtctgggggc cgatcgtcag ggccgagagc cggagccagc ccgtgacctg
     3541 gacgtggacc cggcgcgcca ggagcggatc gcgcgcgagc ttcgcgagct cgcggacaag
     3601 aactaccgcg cgatctacca tctgatccat tcatatatcg gcggcgaccc ggacgaagcc
     3661 gaggacctga ctcttcagac gtttgaaaac gcattcgcct cttatcaccg gttccgaggc
     3721 gaggcagatg tccgaacttg ggtctttcgc atcgccatca acgtgtgcaa gaaccggatc
     3781 cggcagaagc agacccggcg tcggttcgag gccctctcgc tcgacgccac cttcggcggc
     3841 gactcggagg acgacacctc ggccatggac gtgcccgacg tctctcagag ccccacccgc
     3901 atagcggagg gccgcgagct ccgcgagatc ctgcgggcgg gcatccgcaa gctccccgcc
     3961 gactaccgcg aggtgcttct cctcaaactg caggacttct catacaagga gatcgcggac
     4021 atgctagact gcacagtcga gacggttaag tcgcggctct tccgttcgag agtggccctg
     4081 aaacagctgg tgggcgggta cgtggacgag gcggactgac cgcccgaccc cgattcccct
     4141 ggcgagtgtg gccgcccgcg ggtgctgctg ccatgccccc tgtggcttcg cccctggagt
     4201 ctgcaaggaa cattctgcgg tccgggacgt cctagtctcg ggtcacgatc aggcacgaag
     4261 ggactcatcg atgcgagtgg gcgctgagcg cgggactgcc gagccgcagc ctggcgcgct
     4321 cacgttgcgg tggttgatgc tctctgcact gctggcggcg gtcctcacga gcgtggccga
     4381 cgcgcagaag cgcccgattg tacgggagat ccgcatcgac atcgggccac acggccagat
     4441 ctcggaggag gtcgtccgca gtgagctcac ggccgtgtcc gtcggcatgg agtacgacca
     4501 ggccaagacg gccgcggcac tccgagccgt tctcgacacg ggctacttcc agcgtgaccc
     4561 gaagctctcg tatgtagagg ccgttcccca gggcgacgat gtcgccctcg tgttccggct
     4621 gcgcgagaac ccgatcatcg aatcggtcac cgtcgagggg aacacggtgc tggatgacgc
     4681 cacgatcacc gcgctgctca gagaggtgct gcagcccggt cgggtgctca acagccgcat
     4741 ggtggatgag tggatccagg aggctgtatc gcaagcgtac cgtgaccgag gttacgcggc
     4801 cgtcgtgctc agtcctaccc tcagtgagca gggcgtgttg accatcgccg tggtcgagca
     4861 gtacatcgag gatattcggc tcgccggcct gcggaagacg gcggagaagg tggtgcgccg
     4921 ggagattgag gcccggcccg gacagctcgt gaacacgcac cgcatccagg aggacctgga
     4981 tcgcctgctg gcactgggga tcttcgagga cgtgt
//
LOCUS       DAOSPL010000110         5004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_16916_length_5004_cov_3.719943, whole genome shotgun sequence.
ACCESSION   DAOSPL010000110 DAOSPL010000000
VERSION     DAOSPL010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16916_length_5004_cov_3.719943"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /gene="recJ"
                     /locus_tag="PLQ54_03175"
     CDS             complement(<1..1299)
                     /gene="recJ"
                     /locus_tag="PLQ54_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOS92289.1"
                     /translation="MRARLREDWVPRACDEGAVRRLTAETGLLEPTVRVLVQRGVRTA
                     DQASEFVDTPLSALHSPWRIPDMAPAVERVGQAIDSREPILIHGDYDVDGLSATALLT
                     GFLTRLDADVSQYIPHRIEEGYGLGTTAIRQAKESGIRLIITVDCGTRDAHAISQARA
                     AGIDVIVTDHHEPGQSLPPALAVLNPKRHDSAYPFRDLAGVGVAMKLAQALVEKRGLP
                     LTSFFRGYLDLVALGTVADVVPVLGENRVLVRYGLDCLRHTRKTGLRALIRVAGYENK
                     AIDAFAVGFGLGPRLNAVGRLDTGETALRLLLTQDRTEADGLARQLDEVNYERRQEEQ
                     RTTEQALAQVDDRIDLDQERIVVVSAPDWHPGVVGIVASRVRERVYRPAIVLVELEGI
                     AKGSCRSIEGFDISAALDSTRGLLLRHGGHAMAAGLELRLE"
     gene            1424..2839
                     /locus_tag="PLQ54_03180"
     CDS             1424..2839
                     /locus_tag="PLQ54_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92290.1"
                     /translation="MRCVLAAALAACVVGAAPCGANGEAPPGAASQPSGTEAVVRACY
                     AKAAVALSSGKADEFARFLAADYIGPGGATRQALQEELAALLARLVEPEAVFTVESVL
                     ATEQDALAVVRLVLTGFDTTLALPVERVWHELHRWQPIDGGWTLVRASALAGSPVSGA
                     QGAVYSDASTGMRVPVPPGWTAFPCALTAGSQVTLVSPDLAVDFDVMATKLPQEMEPG
                     YVALIGARAMREAIPGYELQDMAQTQLGQWPAVRTRSVYTRGGRMVQADGLLAVHGRQ
                     LYSVGVATCPAERARDYRAQTDQATAAIQWGTPRTGPLPDPRGLINDVRFSHEGLGLS
                     VPIPEGWKAAAGPFWSAEVVFEGYEGQARIVLGCRERSGAATAEDVLNELDDAVRRQA
                     SAFRQLSVEDTSLGEISAVQSLGEFTVGDTSRKRILTCAVTGDVQVFVACDAAPAKAF
                     DRIEPAFRELLKRVQLKAQAE"
     gene            complement(3049..3996)
                     /locus_tag="PLQ54_03185"
     CDS             complement(3049..3996)
                     /locus_tag="PLQ54_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOS92291.1"
                     /translation="MSSAPPDIVGLGLATLDVLLRVPRMATWDDLAWVTSFGLDGGGM
                     VGTACCAAARLGASVGFIGTAGDDLSAQMKLRSFVECGVDLSRLVVRPGPEPNVMIVY
                     VDEATGERIFSPLTGFARYDVQPEALDRDYITSAQILHLDEFNRAAALQAARWMREAG
                     RTVCIDCSRTSSPGPVAPDLLELLGLTDVLISGSGFLRCLTGEPDVRRAGRQALALGP
                     RLVVETDGANGSTTITAEGDFHTPAFTVPVADTTGAGDVFHGAYAVALLRAWEPRLAA
                     QFATAVSAIKCTRLGGRRGIPTLAEVATFLAERGITLPD"
     gene            complement(4001..>5004)
                     /locus_tag="PLQ54_03190"
     CDS             complement(4001..>5004)
                     /locus_tag="PLQ54_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92292.1"
                     /translation="HYAGLYFPTEPEFAADLDAFARAVAVAKGLRNARIGHVGTRPAA
                     FETVAYDEIAMARKFGQTVVPVNLEDIVDDAKAMADDDPKVRAVIADIRQTIPHITVA
                     DDYLLKAAKLETALLAFAEENRIVAMGVQCWPTVFRTLGISLCAIYGRLTQQHLLTAC
                     ETDVLGAVSMLVSDLAAMGETVPHFIDWTIRHREDPNCLLAWHCGNAPPCLARTPGER
                     ALRSRANMTGELPVEPGDHMAGLAQFQLKPGPVTICRLVEYEGEWKMLIAAGEIVPSD
                     ETLAGTWAWVRVPDHDRLYRVLVEEGFIHHASMAHGDHVQALMLACKFLGIRPVRVG"
BASE COUNT          762 a         1621 c         1823 g          798 t
ORIGIN      
        1 ctcgagccgg agttcgaggc cggccgccat ggcgtggccg ccgtggcgga gcagcaggcc
       61 ccgggtcgag tccagcgcgg cggagatgtc aaagccttcg attgagcggc aagatccctt
      121 cgcgatgccc tcgagttcga ccagcacgat agccggacgg tagacgcgct cacggacgcg
      181 tgaggcgacg atgcccacca cgcccgggtg ccagtccggg gcgctcacga ccacgatgcg
      241 ctcctgatcg aggtcgatcc ggtcgtccac ctgggccagg gcctgttcgg tggtgcgctg
      301 ctcctcctgc cgcctctcgt agttcacctc gtcgagctga cgcgccaggc catcggcctc
      361 ggtgcggtcc tgggtgagca gcagccggag ggccgtctcc ccggtgtcga ggcggcccac
      421 cgcgttcagg cggggcccga ggccgaagcc gacggcgaag gcatcgatgg ccttgttctc
      481 gtagcccgcc acgcggatga gcgcccgcag gcccgtcttt cgcgtgtgcc tgaggcagtc
      541 gaggccgtaa cggaccagga cgcggttctc gccgagcacc gggactacgt cggctaccgt
      601 gcccagagcg acgagatcga gatagccgcg gaagaagctg gtgagcggga gcccacgctt
      661 ctcgacgagt gcttgggcga gcttcatggc gacgccgaca ccggccaggt cgcggaaggg
      721 gtaggcggaa tcgtgtcgct tggggttcag caccgccagg gcgggaggga gcgactgtcc
      781 cggctcgtgg tggtcggtga cgatcacatc gatcccggcc gcacgggcct gcgagatggc
      841 gtgggcgtcg cgggtgccac aatcgaccgt gatgatcagg cggatgccgc tctccttggc
      901 ctgccgaatc gccgtggtcc ccaggccata gccctcctcg atgcggtggg gtatgtactg
      961 tgagacatcg gcatcgaggc gtgtcaggaa tccggtgagt aacgccgtag ccgagaggcc
     1021 atcgacgtcg tagtcgccgt gtatgaggat gggctcgcgg gaatcgatcg cttggcccac
     1081 ccgctcgacg gctggggcca tgtcggggat gcgccacggc gagtggagtg cactgagggg
     1141 cgtgtcgacg aactcagagg cctgatcggc agtccggacg ccgcgctgca ccagcaccct
     1201 cacggttggt tcgaggagac cggtctcggc cgtcagccgc cgtacggctc cttcgtcaca
     1261 agcgcgaggc acccagtcct cgcgaagccg agcacgcatg cagaacccct tccgcccctc
     1321 aagcaaccct caggttatac tctcggcgtc tggttttccc ctcggagaag gggtgaggag
     1381 ccctagttct ccccctttcc cttgcatcgg gagtgtgagt gtcttgaggt gcgtactggc
     1441 ggcagcgctg gccgcctgtg tggtcggcgc ggccccgtgt ggggcgaatg gtgaggcacc
     1501 acccggggcg gcgtcccagc cttcgggtac tgaggcggtc gttcgcgcct gctacgccaa
     1561 ggcggccgtg gcgctctcct cggggaaagc tgacgagttc gcccggttcc tggccgccga
     1621 ctacatcggg ccgggagggg cgactcggca agcgttgcag gaggagctcg cggcgctgtt
     1681 ggcgaggctt gtggagcccg aagcggtgtt cacggtggag agcgtgctcg cgacggagca
     1741 ggacgcgctc gcggtggtgc ggctcgtgct gacgggtttt gacacgacgc tggcgctgcc
     1801 tgtcgaacgg gtgtggcatg agctgcaccg ttggcagccc atcgatgggg gatggaccct
     1861 cgtgcgggcg tctgctctgg ccggttcgcc ggtgtccggg gctcagggag ccgtgtactc
     1921 ggacgcctcg accgggatgc gcgttcccgt gccacctgga tggaccgcgt tcccgtgtgc
     1981 gctgactgcg ggtagtcagg tgacactggt ctctcccgac ctcgccgtcg actttgacgt
     2041 catggcgacg aagttgccgc aagagatgga gcctggctac gtggcactga tcggtgcccg
     2101 agccatgcgc gaggccatcc ccggctatga gcttcaggac atggcccaga cccagctggg
     2161 tcagtggcca gccgtgcgga cgcgatccgt ctacacacgg gggggccgca tggtgcaggc
     2221 cgacggtcta ctggccgttc acggccgcca gctctatagc gtcggcgtgg cgacttgccc
     2281 ggcggagcgg gctcgtgact atcgggctca gaccgaccag gcgacggcgg cgatccagtg
     2341 gggtacgccc cgcaccggac cactgccgga tccgcgcggc ctcatcaacg atgtccggtt
     2401 ctcgcacgag gggctggggc tcagcgttcc gatccccgag ggctggaagg cggctgccgg
     2461 cccgttctgg tcggccgagg tggtcttcga ggggtacgag ggtcaggcac gcatcgtgct
     2521 cgggtgccgt gagcgctccg gcgccgctac ggcggaggac gtgctcaacg agctcgacga
     2581 cgccgtgcgg cgtcaggcgt ctgcgttccg tcaactgtcg gtcgaggaca caagcttggg
     2641 cgagatctcc gctgtacaga gcctgggtga gttcacggtg ggggacacat cgcgcaagcg
     2701 aatcctgacg tgcgccgtta ccggggacgt gcaggtgttt gtggcctgcg acgccgctcc
     2761 ggcgaaggcg tttgatcgga tcgagccggc gttccgcgag ctgctcaagc gcgtgcagct
     2821 gaaggcgcag gcggagtgac cgtggggcgc gggtagcaca cgtcggggtt gcggaggggt
     2881 gccatggggc gaaccacgcg ggttcccagc gtggtgggcc cccaacgcgc ccgaagttgc
     2941 ccgtgtgggc ctgcggccga ccgggtcctc cgacaggctc agggcagggg ccccgcgccg
     3001 gcgctcgcca tggcagatgg ctcggtctcc gtcgcactga accggtcatc agtcgggcag
     3061 cgtgatgccg cgctccgcca ggaaggtcgc gacctcggcg agggtcggaa tgccccgtct
     3121 tccgccgagg cgggtgcact tgatcgccga gacagcggtg gcgaactggg ccgccagacg
     3181 gggctcccag gcgcgcagga gcgccaccgc gtaggcaccg tggaacacgt cgcccgcgcc
     3241 ggtggtgtcg gcgaccggaa ccgtgaaggc cggggtatgg aagtcacctt cggctgtgat
     3301 ggtggtgctt ccgttggccc cgtcggtctc cacaacgaga cgcgggccca gagccagcgc
     3361 ctggcggccg gcccggcgca cgtcgggctc acctgtcagg cagcgcagga agcctgagcc
     3421 gctgatgagc acgtcggtca gaccgagcag ttccagcaag tcgggcgcca caggcccggg
     3481 gctgctggtc cgcgagcagt cgatgcacac cgtcctcccc gcctcacgca tccaccgggc
     3541 ggcctgcagc gccgccgcgc ggttgaactc gtcgaggtgc aggatctggg ccgaggtgat
     3601 gtagtcgcgg tcgagagcct cgggctgcac gtcgtaccgc gcaaacccgg tgagcgggga
     3661 aaagatccgc tcacctgtgg cttcgtcgac gtagacgatc atcacgttgg gctcggggcc
     3721 ggggcggacg acgagccggc tcagatcgac gccgcactcg acgaaggacc gcagcttcat
     3781 ctgcgccgag aggtcatcgc ccgccgtgcc gatgaagccc acgctggccc ccagccgggc
     3841 cgcggcgcag cacgcggtgc cgaccatccc gccgccgtcg aggccaaagg atgtgaccca
     3901 ggctagatcg tcccatgtgg ccatccgcgg cacgcggaga agcacatcga gggttgccag
     3961 acccaagccg acgatatcgg gaggcgccga gctcacggcc tcagccgacc cgtaccggcc
     4021 tgatgcccag gaacttgcag gcgagcatca gggcctggac atgatcgcca tgggccatgc
     4081 tggcatggtg gatgaaaccc tcctcgacca gcacgcggta gagccggtcg tgatcgggca
     4141 cgcgtaccca cgcccaagtg ccggcgagcg tctcgtccga cgggacgatc tcgcccgcgg
     4201 cgatcagcat cttccactcg ccctcgtact ccaccagccg gcagatggtg accggcccgg
     4261 gcttgagctg gaactgggcc agtccggcca tgtggtctcc cggctcgact gggagctcgc
     4321 cggtcatgtt ggcacgcgag cggagcgctc gctcgccagg tgtgcgcgcg aggcatggcg
     4381 gcgcattgcc gcagtgccac gccagcaggc agttgggatc ctcgcggtgg cggatggtcc
     4441 agtcgatgaa atggggcacg gtctcgccca tggcggccag atcgctaacg agcatcgaca
     4501 cggcgccgag cacatcggtc tcgcatgctg tcagaagatg ctgttgcgtg aggcggccat
     4561 agatggcgca cagcgagatg ccgagcgtgc ggaagaccgt gggccagcac tgcacgccca
     4621 tggcgacgat ccggttttcc tcggcgaagg ccagtagcgc cgtctcgagc ttggcggcct
     4681 tcagcaggta gtcgtcggcc acggtgatgt gagggatcgt ctgccggata tccgcgatca
     4741 cagcccgtac ctttgggtcg tcgtcggcca tggccttggc gtcatcgaca atgtcctcga
     4801 ggttgaccgg gaccaccgtc tgaccgaact tgcgggccat ggcgatctcg tcgtaggcga
     4861 ccgtctcgaa tgcggccggg cgcgtgccca cgtggccaat gcgggcgttc cgcaggccct
     4921 tggccaccgc cacggcgcgc gcgaacgcgt cgagatcggc cgcgaactcg ggctcggtgg
     4981 gaaagtagag gccggcgtag tgga
//
LOCUS       DAOSPL010000111         4977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_17036_length_4977_cov_3.553637, whole genome shotgun sequence.
ACCESSION   DAOSPL010000111 DAOSPL010000000
VERSION     DAOSPL010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17036_length_4977_cov_3.553637"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="PLQ54_03195"
     CDS             complement(<1..1136)
                     /locus_tag="PLQ54_03195"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS92293.1"
                     /translation="MADDPIDPAPSGPGLSRRHTFSAFRHRNYRLFFFGQLVSVIGTA
                     MQGTALPWLVYTTTQSALWLGIVAALTQLPSLFLSVFGGALVDRLPKRELIVCTQSAA
                     MCLAFTLAALVHWQVVRVWHIAAIALASGLVLAIDIPARQSFVVEMVGREDLMNAIAL
                     NSSCFNMGRILGPVSAGFLILIGMDWCFFLNGLSFVAVIGGLLLMHLPPWQPSRSRPP
                     ILAHTIEGIRYARSQPRIVIALTMMATVAVFGLPYQTLTAVFAHDVLRVGAKGMGLMG
                     GAVGVGALASTLILARRQRPTWTLFLGVMLAMAGGLYCYGLSKAFRLSLAILVGVGAC
                     SVAFTITCNTTLQLASPDAMRGRVMGLYAMSFVGLGPIGSFLSGA"
     gene            1283..1459
                     /locus_tag="PLQ54_03200"
     CDS             1283..1459
                     /locus_tag="PLQ54_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92294.1"
                     /translation="MGTVAVAAAFVAVAGGQVDNGLLFRAAFDGSLIDVTSDAGALAV
                     TIDPATEACVVRVE"
     gene            1751..4450
                     /locus_tag="PLQ54_03205"
     CDS             1751..4450
                     /locus_tag="PLQ54_03205"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOS92295.1"
                     /translation="MPITYTVFMLGALFAAPVAGAGPEGAVAAWQFDADGNGDGIADG
                     WTGRSAAGGATFALVARGAGGAVQQVAGARPGEGISREATGLDPQATYLVTAEVTVTQ
                     GGVLWGPEGVRQKWLGPYGQAVESRATFDGRDRITIAFVADSPGTAFVVSSVRIERAE
                     KAPLPIEADTGRFLVPRPQRIVYEPGETSGVTLAADTPMAFAEIASDRVSLGLFREDL
                     GFAGDATLVEPGHIGDGPTIVVGTPEALRGQAKGLPASLADAVRACPEALREQGYYLR
                     VTPHGVVIAAGDAPSAQYGLQTLRQLCAPAGDGQWRVPVLTIEDWPELPFRATYQVGI
                     QPTPEALARAKYYARMKLNGVVMEDEVLYHLHEGDNLARVREYVQALRDLNLEPIPLV
                     QSFGWGMYVLSIDPTCVEGRYVADRPMRFAARDALPEELREVLPEDGHGREYLVPPEC
                     LAGVPEPFGNTSFEDLDDERPVGWQADRWAGADGVSVSDGDALDGERCLRLNRRSHGT
                     IRVWQDFEVDGATSLQISIQARGTGLTGRGAYAEIYARTEGGELIGAPAAMSNRIRET
                     SDWRPLSMTLEPRGHRWFRVYLRVQEGEGTVWFDDLRAGLAEAQLRNLTHVDESLRLT
                     DDDGNAFTEGRDYEVIAGETRFPFTADAKPWRIVRRPGGRIPAGARVLASYEYAPVGA
                     ITYCPSDPRTQEIMRETLATVIRELGVSRIHIGHDEPRWLNTCRRCRERELSNAELFA
                     DELTRMNDFAKAADPDVRVMMWADALNPHHNAPAQQLEPANDTTPKDMIQCTWTYLAG
                     DDVAEARSLAYMAGRGYETTGSPWFDLENNWDWAQECHLSRQMTGKCLGMIYTSWDDF
                     PEQDAFGGLSVTATFSWNPDDPRALEMLPWSPVEMNRTSGVLP"
     gene            complement(4682..>4977)
                     /locus_tag="PLQ54_03210"
     CDS             complement(4682..>4977)
                     /locus_tag="PLQ54_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92296.1"
                     /translation="DPPLRPFSELDPVARDLVRAAIAASEHPPTIVDATMVRLGLDLS
                     YLVEPLLLTRDEGEIVGGVASLSPSHRPGSQSVAVSSSCPHFLVDPYLLNMLP"
BASE COUNT          834 a         1642 c         1790 g          711 t
ORIGIN      
        1 gcgccgctga ggaagctgcc gatggggccg aggcccacga aggacatggc gtagagcccc
       61 atgacgcgcc cgcgcatggc atcgggtgac gccaactgga gtgtcgtgtt gcaggtgatc
      121 gtgaaggcta cactacacgc ccctacgccg acgaggatgg ccagcgatag gcgaaatgcc
      181 ttggagaggc cgtaacagta cagaccaccg gccatggcga gcatcactcc aaggaagagc
      241 gtccacgtcg gccgctggcg ccgcgcgagg atcaaagtcg atgcgagggc ccctaccccc
      301 accgcgccac ccatcaagcc catgcctttg gcgccgaccc tgagcacatc gtgagcgaac
      361 accgcggtca gcgtctggta gggaagcccg aacacggcca ccgtggccat catcgtcagc
      421 gcgatgacga tccgtggctg gctacgggca tagcggatcc cctcgatggt gtgcgccaga
      481 atgggcggac gcgaccggct gggctgccag ggcggcaggt gcatgagtag taggcccccg
      541 atgacggcaa cgaagctcag cccattgagg aagaagcacc agtccatgcc gatcaagatc
      601 aggaacccgg cagagaccgg cccgaggatg cgccccatgt tgaagcacga cgagttcagg
      661 gcgatggcgt tcatcaggtc ttccctaccg accatctcca ccacgaagga ctgccgagca
      721 gggatgtcga tggccagcac cagacccgag gccagagcga tggcggcgat gtgccacacc
      781 cgcacaacct gccagtggac caacgcggcc agtgtgaacg ccaggcacat ggcggccgac
      841 tgtgtgcaga cgatcagctc acgcttcggg agcctgtcga ccagcgcgcc accgaacacc
      901 gagaggaaca gcgacgggag ctgcgtcaga gcggccacaa tgccgagcca gagggcggat
      961 tgggtcgtcg tgtagacgag ccacggcaac gccgtcccct gcatcgccgt gccgatgacc
     1021 gacaccagct ggccgaagaa gaagagccgg tagttgcgat gacggaaagc cgagaacgtg
     1081 tgccgtcgcg agagtccggg cccggacggc gcgggatcga tgggatcgtc agccatggtg
     1141 cgtcacttcg gcccggccgg ccgttgccct gctcaatgga ccgcccagga gccgcgcgcc
     1201 gcgctcgcga acctgctacc catcgggacc cgggtggcac ttgcggcaag agggcacgcc
     1261 aacaccaggg gaggcggtca gcgtgggcac cgtcgctgtc gcagcggcgt tcgtggccgt
     1321 ggcgggcggt caggtggaca acgggctgct gttccgggcc gcgttcgacg gcagtctcat
     1381 cgacgtcacc agcgatgcgg gcgcgctggc cgtcaccatc gacccggcga ccgaggcgtg
     1441 cgtggtgcgg gtggagtagg ggcatcggcg ccaactcagg ctcggttgag gggtggacgc
     1501 ctcagtcgcg cgcccggagg cctcggccgt tagcgatacc agcgggtgca ggtccttgta
     1561 gtcccccacg ccgtcgcgca ggagctcgag gcgtaccggc ggccacacgc ccgcgccgcc
     1621 gcgcgcgggg gcggtgtcaa tggaccaggt gcctcgccgc atcgcagctg gagggatgcc
     1681 tctggtcggt gtcgaacagc aagtcagcac tacctagact gcactatctc ctgtctgtga
     1741 ggagcctcag atgccgatca cgtacaccgt cttcatgctg ggcgctctgt ttgccgcccc
     1801 tgtcgcagga gctggccccg agggagccgt cgctgcttgg cagttcgatg ccgatggcaa
     1861 cggcgatggc atcgccgacg gctggaccgg ccgctcagcg gccgggggcg cgacgtttgc
     1921 cctggtcgcc cgcggcgccg gtggcgcggt tcagcaagtc gcgggcgccc gcccgggcga
     1981 ggggatctcg cgcgaggcca ccgggctcga cccacaggcc acctacctgg tgaccgccga
     2041 ggtgacggtg acgcagggcg gcgttctgtg gggacccgag ggcgtgcggc agaagtggct
     2101 cgggccgtac ggccaagctg tggagagccg ggcgaccttc gacggtcgag accggatcac
     2161 catcgccttt gtcgccgaca gcccgggcac cgccttcgtc gtctcgagcg ttcgcatcga
     2221 gcgggccgag aaggccccgc tgcccatcga ggcggacacg ggccggttcc tcgtgcctcg
     2281 gccgcagcgc atcgtctatg agcctggtga aacctccggc gtcacgctgg ccgccgacac
     2341 gccgatggcg ttcgccgaga tcgcatccga ccgggtgagc cttgggctgt tccgggagga
     2401 cctgggcttc gccggggacg ccacgctggt cgagccgggc cacatcggcg acgggcccac
     2461 catcgtggtc ggcactcccg aggcgctgcg cggtcaggcc aagggcttgc cggcctcgct
     2521 cgccgatgcg gttcgagctt gcccggaggc actgcgggaa caagggtact acctgcgggt
     2581 cacgccccat ggcgttgtca tcgcggcggg cgacgccccg tcggcccagt atggcctgca
     2641 gacgctgagg cagctctgcg cgccggccgg cgatgggcaa tggcgcgtgc ccgtgctgac
     2701 catcgaggac tggccggagc tgcccttccg ggccacgtat caggtcggga tccagcccac
     2761 gcctgaggcc ctggcgaggg cgaagtacta cgcccggatg aagctgaacg gcgtggtgat
     2821 ggaggatgag gtgctctatc acctccacga gggagacaac ctggcgcggg tgcgcgagta
     2881 cgtccaggcc ctccgtgacc tcaacctcga gccgattccc ctggtgcaga gcttcggctg
     2941 ggggatgtac gtgctgagca tcgacccaac gtgcgtcgag ggccgctacg tggccgatcg
     3001 gcccatgcgc ttcgccgcac gcgatgcgct gcccgaggag ctccgcgagg tgctgccgga
     3061 agatggccat ggccgggagt acctggtgcc tcccgaatgc ctggcgggcg ttcccgagcc
     3121 attcggcaac acctcattcg aggacctgga cgacgagcgc ccggtcgggt ggcaagccga
     3181 tcgctgggcg ggcgcggacg gagtgtcggt gagcgatggg gacgcgctcg atggcgagcg
     3241 gtgcctccgg ctcaaccggc gtagccatgg caccatccgg gtgtggcagg acttcgaggt
     3301 ggatggcgcc acgagccttc agatcagcat ccaagcccgg ggaactggcc tcacgggcag
     3361 gggcgcctat gccgagatct acgcccggac cgagggcggt gagctcattg gagcgcctgc
     3421 cgccatgtcg aaccggatcc gcgagaccag cgactggcgg ccgctcagca tgactctcga
     3481 gcccagaggt caccgctggt tccgtgtgta cctgcgggtc caggagggcg agggtacggt
     3541 gtggttcgat gacttacggg cggggcttgc ggaggcgcaa cttcggaacc tgacccatgt
     3601 cgacgagagc ctgcgcctca cggacgatga cggcaacgcg ttcaccgagg gccgcgacta
     3661 tgaggtcatc gcgggcgaga cgcgcttccc cttcacagcg gacgccaagc cgtggcggat
     3721 cgtgcggcgg cctggtggcc gaatcccggc cggagcgcgg gtgctcgcga gctatgagta
     3781 cgcgccggtg ggggccatca cgtactgccc gtcggacccg cgaacgcagg agatcatgcg
     3841 cgagaccctg gccacggtca tccgcgagct cggcgtgagc cgcattcaca tcggccacga
     3901 tgagccgcgc tggctgaaca cgtgccggcg ctgccgcgag cgcgagctca gcaacgcgga
     3961 gctgttcgcc gacgagctga cgcggatgaa cgacttcgcc aaggccgccg accccgacgt
     4021 gcgcgtgatg atgtgggccg acgcgctcaa cccccaccac aacgccccgg cgcagcagct
     4081 cgagccggcg aatgacacga cgccgaagga catgatccag tgcacgtgga cctacctcgc
     4141 cggcgacgat gtggcggaag cgcggagctt ggcctacatg gccgggcgtg ggtacgagac
     4201 gacggggtcg ccctggttcg acctcgagaa caactgggac tgggcccagg agtgccacct
     4261 gagtcgccag atgacgggca agtgcctggg gatgatctac acctcatggg acgacttccc
     4321 ggagcaggat gcgtttggcg gcctctcggt gaccgccact ttctcgtgga atcccgacga
     4381 cccgcgggcg ctggagatgc tcccctggtc gccggtggag atgaaccgca cctccggcgt
     4441 gctgccgtag ctggcgtgcg agtgccggca ggcccgcgcc cggtgctgag gaggccctgc
     4501 ggaggggccg aatggggcgg gggccccatc cggcccgccc tattggccgc gagccgatcc
     4561 gccggtcggc tctggtggct caggcggctc gcccgagagc cgaaggccgc cgctcgggac
     4621 ccagcgacgc cctcggggaa cgccggctga accaatggtg tgccgtcgcc cgcttccacc
     4681 tctacggcag catgttgaga aggtaggggt cgacgaggaa gtgcgggcac gaggagctca
     4741 cggccacgct ctgcgaccca ggccggtgac tggggctcag tgatgcgacc cccccgacta
     4801 tctcgccctc gtccctggtc agcagtaggg gttcgaccag gtagctcagg tcaagcccga
     4861 ggcggaccat cgtggcgtcc acgatcgtcg gcgggtgttc gcttgcggca atggcggccc
     4921 gaaccagatc gcgggcgacc ggatcgagct ccgagaacgg gcgcagggga ggatcct
//
LOCUS       DAOSPL010000112         4936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_17216_length_4936_cov_3.956566, whole genome shotgun sequence.
ACCESSION   DAOSPL010000112 DAOSPL010000000
VERSION     DAOSPL010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17216_length_4936_cov_3.956566"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PLQ54_03215"
     CDS             complement(<1..523)
                     /locus_tag="PLQ54_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92297.1"
                     /translation="MAEHMDAVVVGAGPAGCAFAVGFASGGGCVTLVDGSGQGSKPCG
                     GAFPPAQLSETVSGALAATKPLWSVPSRVQFETADGQSESWPVPQGALVVCARQRLDE
                     ALRNCAAEAGARLVRGRATNIHRADGRWRLQVGRTALSAGLLVGADGAAGSHGVREAL
                     GLPPTRGLWARISH"
     gene            complement(534..749)
                     /locus_tag="PLQ54_03220"
     CDS             complement(534..749)
                     /locus_tag="PLQ54_03220"
                     /inference="COORDINATES: protein motif:HMM:NF038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Franean1_4349 family RiPP"
                     /protein_id="HOS92298.1"
                     /translation="MSREGVQKLIGLAVTDVAFRERLLDDPAAAATEYGVDLTDQEIE
                     NIRKVHPADINAFADAFVARFGDRAAY"
     gene            complement(787..1698)
                     /locus_tag="PLQ54_03225"
     CDS             complement(787..1698)
                     /locus_tag="PLQ54_03225"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 isoprenoid biosynthesis enzyme"
                     /protein_id="HOS92299.1"
                     /translation="MRARVGGLAEPLASLARPFVDGYGEATWSHPYSFPLAFMPWWIL
                     DAAGRLPQTGVEFERAVDYAYGTMAGYLYIRIQDDIYDEPDRPSDPRLLLVANELICE
                     SLCAFGRHQTPEGRFWSCFRRYWEDFGQATLWQMDAHRGRHTIPDESDLVRSGRRLSL
                     AKAPCAAVLLDLGGTSRMEQMDQGMDLLQAANQLANDVASVTRDIESRNVTYPIALAT
                     GPEFDWTAADYRESALRCVLRSEAVERALERAADLHRRALAVMDGLASPWLDAFVSAR
                     IELLSCGAKEFTGLRLESLFGLMRPMD"
     gene            complement(1784..2686)
                     /locus_tag="PLQ54_03230"
     CDS             complement(1784..2686)
                     /locus_tag="PLQ54_03230"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOS92300.1"
                     /translation="MASANPFSFPGRLLKGNLHTHTKRSDGTLSPEETVAWFRDRGRY
                     DFVVLTDHRVLAETDALCSEGFTVVPGVELHPGKNELGHAHHIVCVGVTGPAELPGLD
                     SPQEVLTTVDEAGGVCFAAHPRWHGCTFRDLIALEGYAGVEVFNTTCSALNGKGDSTV
                     IWDDLLARGRRVFGVAVDDAHLARMDYFGGWVMVRTDDASPDGIVAALKAGAFYSSCG
                     PEIRSLEVTQSHLKLECSPVRSVHVVSRGPVGVSFMDPGGALMDGVDCTLGGMDTYLR
                     VQVVDASGMSAWTNPFYLDELPPE"
     gene            complement(2731..4125)
                     /gene="cysS"
                     /locus_tag="PLQ54_03235"
     CDS             complement(2731..4125)
                     /gene="cysS"
                     /locus_tag="PLQ54_03235"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOS92301.1"
                     /translation="MNTVRLYNTLSRRVEELVPVTPGKIGLYCCGPTVYDYAHIGNLR
                     KYVCDDLLRRTLEHAGYEVRHVMNVTDIDDKIIKAAPGGIEAIRRHTAPFREAFFEDT
                     AALNILRPHVVCAATEHIDAMAALVAKLVESGHAYESEGSYYFRIAAYPEYGRLARLD
                     TAGMMAGARVDVDEYEKGDVRDFALWKAARPGEPAWETPLGPGRPGWHIECSAMSMQY
                     LGEEFDIHTGGVDNMFPHHENEIAQSCATGAGFVRVWLHHEHLLVEGRKMAKSLGNFF
                     TLRDLLSRGHDPRGIRYLYLSHHNRSQLNFTFDGLAAAERTVHGLVQFMDRLEDALPS
                     LEPGTTALAGRVAAARQGFFDALYDDLQTPQALARLHDMVRDVNASLADGSARAGDVE
                     GAREALEDFDSILGVLAHERASLEEDVERLIAERQEARARRDFARSDAIRDELLARGI
                     VLEDTPAGVRWRRV"
     gene            complement(4122..>4936)
                     /locus_tag="PLQ54_03240"
     CDS             complement(4122..>4936)
                     /locus_tag="PLQ54_03240"
                     /inference="COORDINATES: protein motif:HMM:NF016203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HOS92302.1"
                     /translation="EAAAAPRQSVDASHLVLGVECGGSDAFSGLSANPALGVAADLLV
                     ALGGTVILSETSEMFGAERDLLSRARDDAVRQRIVDFISREEEFVASLGLGDAAGSVT
                     LAPGNEDGGLTTIEEKSLGCVRKGGRSTIQDFVEYGARVRGPGLFLMDTPGHDVRSMT
                     GMAAGGANLMGFTTGRGTPTGHATVPVLKIGSNPQVFMDMPDVIDVNAGSIVEGTCSV
                     EAVGREILRHLLRVASGELTNAERNGHAEFSIAPPHHPRSANPTIGCQEACP"
BASE COUNT          874 a         1761 c         1554 g          747 t
ORIGIN      
        1 catgggagat gcgcgcccac aggccgcggg tgggcggcag accgagcgcc tcgcggacgc
       61 catggcttcc cgcggcgcca tcggccccga caagcaggcc ggcggagagc gcagttcgcc
      121 caacctgcaa ccgccaacgg ccatcggcgc gatggatgtt cgtggcgcgg ccgcggacca
      181 ggcgagcccc ggcctccgcc gcacaattgc gcagcgcctc atccagccgc tggcgggcac
      241 acacaaccag ggcaccttgt ggcacgggcc acgactcgct ctgcccgtct gccgtctcga
      301 actggactcg agacgggacg gaccagaggg gctttgtggc cgccagggcc ccggagacgg
      361 tctcggaaag ctgcgccggc gggaaggcgc cgccgcatgg cttggagccc tgtcccgagc
      421 cgtcgacgag cgtgacgcag cctccaccgg acgcgaagcc cacggcgaag gcgcacccgg
      481 ctgggccggc ccccaccaca actgcgtcca tgtgttcggc caaacagacc gcctcaatag
      541 gctgcccggt cgccgaaccg ggcgacgaag gcgtcggcga atgcattgat atcggccggg
      601 tgcaccttgc gtatgttctc gatctcctga tcggtgaggt cgaccccata ctcagtggct
      661 gctgcggcgg gatcatcaag caaccgctcg cgaaacgcca catcggtgac ggcaaggcca
      721 atgagcttct gaacgccttc gcgactcacg agagcacctc cgcggaactc cagcgcgccc
      781 accgtctcaa tccatcgggc gcatcagacc gaataggctt tcgagcctca gacccgtgaa
      841 ctccttcgcc ccacacgaga ggagctcgat ccgtgcggac acgaaggcat ccaaccacgg
      901 cgaagccagt ccgtccatga cagccagggc cctgcggtgc agatcggcgg ccctctcgag
      961 tgcccgctcc acggcctcgc tccgcagaac gcagcgcagc gccgactcgc ggtagtccgc
     1021 ggcggtccag tcgaactccg gccccgtcgc gagcgcgatg gggtaggtca cgttgcgcga
     1081 ctcgatgtca cgggtaacgc tggccacgtc gttcgccagc tggttcgcag cctggaggag
     1141 atccatgccc tgatccatct gctccatccg cgatgtgccg cccagatcga gaagcaccgc
     1201 agcgcatggg gccttggcca gcgagagcct tcggccgcta cgcacgagat ccgactcatc
     1261 ggggatcgtg tggcggccgc ggtgcgcatc catctgccac agcgtggcct gcccgaagtc
     1321 ctcccagtag cggcgaaagc agctccagaa ccggccctcg ggcgtctgat gccgcccgaa
     1381 ggcgcaaagc gactcgcaga tgagctcgtt ggccaccagc agcaggcggg ggtccgatgg
     1441 ccggtccggt tcatcgtaga tgtcatcctg gatacgaatg taaagataac ccgccatggt
     1501 gccataggca tagtcgaccg cgcgctcaaa ctcgaccccg gtctgtggga gccgtcccgc
     1561 cgcgtccagg atccaccacg gcatgaatgc cagagggaac gagtaagggt gagaccacgt
     1621 cgcctctccg tagccatcga caaacggccg ggccaatgaa gccagcggct cggccaggcc
     1681 cccaactcgc gctcgcaacc gatggcacgc agcagccagg tgggaggcga acgcctggta
     1741 gaaggggcga tccagccacc cggggagctg gccggactct cgatcactcg gggggtagct
     1801 catcgaggta gaaggggttc gtccacgcag acatcccact ggcatcgacg acctggactc
     1861 tcaggtaggt gtccatcccg cccaacgtgc agtccacgcc gtccatgagg gcgccgccgg
     1921 ggtccatgaa gctcacccca acagggccac ggctgaccac atgcacggac cgcaccggag
     1981 agcactccag cttcaggtgc gactgggtca cctccaggct gcggatctcc ggcccacaag
     2041 atgagtagaa cgcacctgcc ttgagggctg ccacgatccc atccggcgag gcgtcgtcgg
     2101 tgcggaccat gacccagccc ccgaagtagt ccatccgggc caggtgcgca tcatccaccg
     2161 ccacaccgaa cacacgtcga cctcgcgcca gcaggtcatc ccagatcacg gtcgagtcgc
     2221 ccttgccgtt gagcgcgcta caggtggtgt tgaacacctc gacgccggcg taaccctcga
     2281 gcgcgatgag gtcacggaaa gtacacccgt gccatcgcgg gtgggccgcg aagcagacgc
     2341 cgcccgcctc gtcaaccgtg gtcaggacct cctggggcga gtcgagtccg ggcagttcgg
     2401 ccgggcccgt cacgccgaca cacacgatat ggtgggcgtg ccccaactcg ttcttgccgg
     2461 ggtggagctc cacgccagga acgacggtga aaccctcgga gcagagcgcg tcggtttcgg
     2521 ccagcacccg atggtcggtc aacaccacga aatcataccg gcctcgatcg cggaaccacg
     2581 ccaccgtctc ctctggcgag agcgtcccat cggagcgctt ggtgtgagtg tggaggttcc
     2641 ccttaaggag cctgcccgga aagctgaacg ggttcgcgct agccacgagc caccccaatc
     2701 cgcactggac cgccccatgg gcggcattcc ctatacccga cgccaccgga cgcctgccgg
     2761 cgtgtcctca agcacgatgc cccgggcgag gagttcatcg cgaatcgcgt ccgaccgggc
     2821 gaagtcccgc cgggcccggg cctcctgccg ttcggcgatc aggcgctcca catcctcctc
     2881 cagcgaggct ctctcgtgcg ccaggactcc caggatcgag tcgaagtcct ccagtgcctc
     2941 gcgggcgccc tcgacatcgc cggcccgcgc cgacccgtcc gccagcgatg cgttcacatc
     3001 gcggaccata tcatgcaggc gcgccagcgc ctgcggcgtc tgcaggtcgt cgtagagcgc
     3061 gtcgaagaac ccctgtcgcg ccgcggccac acgtccggcc aaggccgtgg ttcccggctc
     3121 caggctcggc aacgcatcct cgagccggtc catgaactgg acgagaccat gcacagtgcg
     3181 ctcggccgcc gccaagccgt cgaatgtgaa gttgagttgc gaccggttgt ggtgcgagag
     3241 atagaggtac cggatgcccc tggggtcgtg ccctcttgag agcaagtcgc gtagggtgaa
     3301 gaagttgccc agtgacttgg ccatcttccg cccctcgaca agcaggtgct cgtgatggag
     3361 ccacacccgc acgaaccctg cgcccgtggc acaggactgc gcgatctcgt tctcgtggtg
     3421 cgggaacatg ttgtcgaccc cgcccgtatg gatgtcgaac tcctcgccca ggtactgcat
     3481 ggacatggcc gagcactcga tgtgccagcc gggccgtccg ggcccgaggg gcgtctccca
     3541 cgccggttca ccaggccggg cggccttcca caacgcgaag tccctcacgt cgcccttctc
     3601 atactcgtcg acatcaaccc tcgcgcccgc catcatgccc gcggtgtcca gccgcgccag
     3661 ccggccatac tcgggatagg cggcgatccg gaagtagtac gaaccctcac tctcgtaggc
     3721 atggcccgac tcgacgagct tggccacgag tgcggccatg gcatcgatgt gctcggtggc
     3781 cgcgcagacg acgtgggggc gcaggatgtt cagcgcggcg gtgtcctcga agaatgcctc
     3841 gcggaacggc gcggtgtggc gccggatcgc ctcgatccca cccggcgccg ccttgatgat
     3901 cttgtcgtcg atgtctgtca cgttcatcac atgccggacc tcatagccgg catgctccag
     3961 cgtccggcgc aacaggtcat cgcacacgta cttgcggagg ttgccgatgt gagcgtagtc
     4021 atacaccgtg gggccacagc agtagaggcc gatcttgccc ggcgttaccg ggaccagctc
     4081 ctccacgcgg cgcgacaagg tgttgtacag cctcacggtg ttcatgggca ggcctcctgg
     4141 caaccgatgg tcgggttcgc actcctcgga tggtgcggtg gggcaatcga gaactcagcg
     4201 tggccgttac gttccgcgtt ggtcagctcg cccgaggcca cacgaagtag atgccggagg
     4261 atctctcgcc ccaccgcttc caccgagcat gtgccctcaa caatagaccc cgcattcaca
     4321 tcaatgacgt cgggcatgtc catgaacacc tgcgggttgc tcccgatctt gaggaccggc
     4381 actgtggcat gccccgtggg cgttccacgc cccgtggtga agcccatcag gttggccccg
     4441 ccggccgcca tcccggtcat ggaccggacg tcgtgcccgg gcgtatccat caggaacagc
     4501 cccgggcccc gtacgcgtgc cccatactca acgaagtcct gtatcgtcga ccggcctccc
     4561 ttgcgcacgc agccgagcga cttctcctcg atggtcgtca agccgccgtc ctcattcccc
     4621 ggagcgaggg tcacgctccc ggccgcatcg ccgagcccca gggaggcgac gaactcctcc
     4681 tcgcgcgaga tgaaatcgac aatccgctgc cgcaccgcgt catcacgggc gcgagagagc
     4741 aggtcgcgct cggcaccgaa catctcactc gtctccgaga ggatcaccgt tccccccagc
     4801 gccaccagca ggtcggcggc gaccccgagc gccgggttcg ccgacagccc cgagaacgcg
     4861 tccgagccac cgcactcgac gcccagcacc aggtgactgg cgtccacgga ctggcggggc
     4921 gcggctgcgg cctcgg
//
LOCUS       DAOSPL010000113         4897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_17391_length_4897_cov_2.878563, whole genome shotgun sequence.
ACCESSION   DAOSPL010000113 DAOSPL010000000
VERSION     DAOSPL010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17391_length_4897_cov_2.878563"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..585)
                     /locus_tag="PLQ54_03245"
                     /pseudo
     CDS             complement(139..585)
                     /locus_tag="PLQ54_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014464138.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(582..1130)
                     /gene="hslV"
                     /locus_tag="PLQ54_03250"
     CDS             complement(582..1130)
                     /gene="hslV"
                     /locus_tag="PLQ54_03250"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOS92303.1"
                     /translation="MSRDTGARMHGTTIVAVRRNGVLAIAGDGQVTLQETILKATAQK
                     VQRIFRGRVVAGFAGSVADAVTLLERCEQKLEESSGNLQRAVHALARDWRTDRMLRRL
                     EAMLLVGDSEHLFLVSGQGDVLRPEDDIAAIGTGGAYALAAAKALAKHTQMGAEAIAT
                     EALGIAAGICVYTNDTIHVETI"
     gene            complement(1155..2048)
                     /locus_tag="PLQ54_03255"
     CDS             complement(1155..2048)
                     /locus_tag="PLQ54_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HOS92304.1"
                     /translation="MEEQASAFAAALMGRGLSRHTVKAYSTDVREFLEYAGQTEASGL
                     ADVGTVRRYVASLHRLGRKKRTVARKLAAVRSFLEYLRERGDLTLNPAALVRGPKLDK
                     ALPDFIYSEHIGALLGAPDDSPAGLRDRALLEVMYATGLRVGEVASLTVDQLDGSSDH
                     LRVVGKGSKERMVLVGRYALAAVEAYRARGRPVLAARSKRIEDLGRKDLWLNSRGGRL
                     SVRGIERIVRKYALRVGERAQTSPHTLRHSYATHMLENGADLRAIQELLGHSSLSTTQ
                     IYAHVTLRSLRDAYDQAHPLA"
     gene            complement(2032..3360)
                     /gene="trmFO"
                     /locus_tag="PLQ54_03260"
     CDS             complement(2032..3360)
                     /gene="trmFO"
                     /locus_tag="PLQ54_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOS92305.1"
                     /translation="MGGSAGIRVIGGGLAGCEAAWQLASRGVAVELVEMRPLVGTAAH
                     ATSGLAELVCSNSLRSNALDRAQGILKEEMRRLGSLIISCADECAVPAGSALAVDRVR
                     FSSRVESKLLALETVHLVRAEAREVCGDGVTTIVATGPMTSPAMSEAIRAVVGRGFLH
                     FYDAVSPIVSAESVDRSVAFEAARHGKGEATYLNCPLSKEEYERFVAELTAAAVSPTR
                     EIAPGAFFEGCLPVEEIARRHPDALRHGPLRPVGLTDPRTGRWPYAVVQLRPENAAGT
                     LYNLVGCQTGLRWGEQERVFRMVPGLTEAEFLRYGVMHRNVFIESPALLDPFCRLVTD
                     PRIAFAGQITGVEGYCESALSGLYVGLNSFRVAEGLDPVRLPECSVSGALLSHVTSAR
                     SGEFQPMNANFGLVPPVEGVAKRDRRAAMARRALEAIDEFARSVGCGGAG"
     gene            3444..4106
                     /locus_tag="PLQ54_03265"
     CDS             3444..4106
                     /locus_tag="PLQ54_03265"
                     /inference="COORDINATES: protein motif:HMM:NF022200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 family protein"
                     /protein_id="HOS92306.1"
                     /translation="MTRREFGKLAATGLPMLGASLAAANDDVIEGYFGAANDHLRRIR
                     SDRAGQIGRAARRLAGRVAEGGRLFIFDSRGGYVSEALGRAGGLMAIAALPADAAGVT
                     ARDAVIVVADDRPDAAAIASAQAARAAGAFVAGIGPATGTDNSLAAACHATIDNTLDG
                     GAGNPLSGVLNTAILWALIAGYIEAMEARGKPPHIWMSIKRPGSKAFNDAALADTAKV
                     GY"
     gene            4109..4666
                     /locus_tag="PLQ54_03270"
     CDS             4109..4666
                     /locus_tag="PLQ54_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92307.1"
                     /translation="MGYAEAYLDAVRANLEKVAQREAERLRQAAARIAGAIAAGHRAF
                     EFLQGHFLPIEAARGHEDRPLVFTPITLPDAAHLRPGDVLVMSHQYGVLPECVDMAFA
                     ARRHGAYIVAMCPPSDPAVIVRSHPSGKSVPDLADLVISTHIPAGDVALQVPAGGPGA
                     CPTSGTIQAAMYWALVCAVAERLRP"
BASE COUNT          825 a         1839 c         1494 g          739 t
ORIGIN      
        1 gcgtcagcga cgggcagctg ctcttccttc aaaacctgct ggccaaggcg gaggcggagc
       61 gggcggagac tgcggctgga acaggccctg taggatcccc gggaacccga tggccgtccc
      121 ctcccgcggc cgcttgcgcg ccgccggatc cggctccatt agatccacca ggcggtccag
      181 ggcgatctcc ttggcccggg cgcggacctg ttcgaggcac tcagcccgca ccatgtggat
      241 ggccacctcg accaagtcgc ggaccatgga ctccacgtcc cgccccacgt acccgacttc
      301 cgtgtacttg gtggcctcga ccttgacaaa cggagcccgc gccaacgatg ccagccttcg
      361 ggcgatctcc gtcttgccca ctcccgttgg cccaaccatc aggatgttct tcggcataac
      421 ctcctcgcgc agttcgtcgg gcagcaagcg cctccggtag cgattccgca gggcaatggc
      481 caccgcccgc ttggccttgg cctgcccgac aatgtactgg tccagcgcct ccacgatgcg
      541 ctgcggcgtg aggtcctgat cgcatccatc ccgctggggg ttcatatcgt ctccacatga
      601 atcgtgtcgt tggtatagac gcagatgccc gccgcaatgc ccagcgcctc ggtggcgatg
      661 gcctcggcgc ccatctgagt gtgcttcgcc agcgccttgg ccgccgccag ggcataggca
      721 ccgcccgtgc caatggcagc aatgtcatcc tcgggtctca acacgtcccc ctggcccgac
      781 accaggaaca gatgctcgga gtcgcccacc agcagcatcg cctcgagtcg acggagcatg
      841 cggtccgtgc gccagtcccg agccagtgcg tggacggccc gctggagatt cccactgctc
      901 tcctccagct tctgttcgca acgctccaac aaggtgaccg catccgccac actgccagcg
      961 aagccggcca cgacccggcc gcggaagatc cgctgcacct tctgcgccgt cgccttgaga
     1021 atggtctcct ggagagtcac ctggccatcc ccggcgatgg ccagaacgcc gttccggcgc
     1081 accgccacga tggtggtgcc gtgcatgcgt gctcccgtgt cccgcgacat cagtggcgcc
     1141 tccctcccat ccatctacgc cagggggtgg gcctggtcat aggcatcccg cagactccgc
     1201 aaggtgacat gggcatagat ctgagtcgtc gacagactgc tgtgccccag tagctcctgg
     1261 atggccctca gatcggcccc gttctcgagc atgtgtgtcg catagctgtg ccgcaatgtg
     1321 tgtggtgagg tctgggcacg ctccccgacc ctcagggcat acttccgcac gatccgctcg
     1381 atcccccgaa ccgagagacg cccacctcgg ctgttcagcc aaaggtcctt ccgtcccagg
     1441 tcctcgatcc tcttcgatcg tgccgccagg accggccgcc cccgcgcccg atacgcctcc
     1501 acagcagcca gagcgtaccg gccgaccaac accatccgct ccttacttcc cttacccacc
     1561 acgcggaggt ggtccgagga gccgtcaagc tgatcgacgg tcagcgacgc cacttcgccc
     1621 acccgaagcc ccgtggcgta catcacctcg agcagtgccc ggtcccgaag acccgccggc
     1681 gagtcgtctg gcgcccccag aagcgcgcca atgtgttcgg agtagatgaa gtcgggcaac
     1741 gccttgtcga gcttcgggcc ccgcaccaga gccgcaggat tgagcgtgag atcccctctc
     1801 tcccgcagat actcgaggaa gctccgcacc gctgccagct tccgcgccac cgtgcgcttc
     1861 ttccggccca gtctgtgcag tgaggccaca taccggcgca ccgtacccac atccgccagt
     1921 ccgctggcct ccgtctgtcc ggcatactcg agaaactccc gaacgtcggt cgagtaagcc
     1981 ttcaccgtgt gccgcgacag gccccggccc ataagggccg ccgcgaacgc gctagcctgc
     2041 tcctccacac cccacgctcc ttgcgaactc gtcgatggcc tccagcgcgc ggcgagccat
     2101 cgcggcccga cgatcccgct tcgccacccc ctctaccggc ggcaccagcc cgaagttcgc
     2161 gttcatgggt tggaactctc cggatctcgc cgaggtcacg tgcgacaaca gggcccccga
     2221 cacactgcat tccggcagcc gcaccggatc gagcccctcc gcgacccgaa acgagttcag
     2281 ccccacgtac agccccgaga gcgctgactc gcagtacccc tccacgccgg tgatctgccc
     2341 cgcgaatgcg atacggggat cggtcaccag ccgacagaac ggatccagaa gcgccgggct
     2401 ctcgatgaaa acgttccggt gcatgacccc gtagcgcaga aactccgcct cagtcaaacc
     2461 cgggaccatc cggaagaccc gctcctgctc gccccaacgc agccccgtct gacaccccac
     2521 caggttgtac agcgtcccgg ccgcgttctc gggccgcagc tggaccaccg catagggcca
     2581 acggccggtt cgcgggtccg tcagcccaac cggcctcaac ggaccgtgtc gcagggcgtc
     2641 ggggtgccgc cgcgcgatct cctcaaccgg caggcaaccc tcgaagaacg cccccggcgc
     2701 gatctcgcgc gtgggcgaaa ccgccgctgc agtcagctcg gcgacgaagc gctcgtactc
     2761 ctccttcgac agcgggcagt tgagataggt cgcctcgccc ttaccgtgcc gggccgcctc
     2821 gaaagccaca gaccggtcca ccgactccgc cgacacgatc ggcgagaccg catcgtagaa
     2881 gtgcaggaaa ccgcggccta cgacggcgcg gatcgcctcg gacatggcgg gagacgtcat
     2941 cggaccggtc gcgacaattg tggtcactcc atccccacaa acctcccgcg cctccgcacg
     3001 caccaggtga accgtctcaa gcgcgagcag cttcgattcc acgcgactcg agaaccgcac
     3061 tcggtccacg gccagtgccg agcccgccgg gacggcgcac tcatcggcac acgagatgat
     3121 cagcgaccca agccgccgca tctcctcctt caggatgccc tgggcccggt caagggcgtt
     3181 cgagcgaaga gagttactgc agaccaactc agccaggccg ctggtcgcat gggcggccgt
     3241 gccgaccagt gggcgcatct ccacgagctc cacggccacc ccgcgcgatg ccaactgcca
     3301 ggccgcctcg caacctgcca gcccgccgcc gatgactcga atgcccgccg atccacccat
     3361 ggtggagcga ttatacgtca ccgccccgcg caggcggctc ggcacccctc accgaatccg
     3421 cgagcgatgg aggtggtacg cccatgacac ggcgcgagtt cggaaagttg gccgcgacgg
     3481 gactgcccat gctcggcgca tccctcgccg ctgcgaacga cgacgtcatc gagggctact
     3541 ttggggccgc gaatgaccac ctgcgccgca tacggtccga ccgggccggc cagatcggcc
     3601 gcgcggcgcg gcggctcgcc ggacgggtgg cggagggagg acgcctcttc atcttcgact
     3661 cccgaggggg ctatgtgtct gaggcgctgg ggcgggccgg cggactcatg gccatcgcag
     3721 cgctcccggc ggacgccgct ggagtcaccg ccagggacgc ggtgatcgtt gtggccgacg
     3781 accggccgga cgccgccgcc atcgccagcg cccaggccgc tcgtgcagcc ggcgccttcg
     3841 tggcgggcat cggcccggcc accggcaccg acaacagcct ggcagcggcg tgccacgcca
     3901 ccatcgacaa cacccttgat ggcggcgccg gcaaccctct ctccggggtg ctcaacaccg
     3961 ccatcctctg ggcgctcatc gccggctaca tcgaggccat ggaggcccgg gggaagccgc
     4021 cacatatctg gatgagcatc aagcgcccgg gatccaaggc tttcaacgac gcggctctgg
     4081 ccgacacggc gaaggtgggg tactagccat gggctacgcc gaagcctatc tggatgcggt
     4141 ccgtgccaac ctggagaagg tggcccagcg cgaggcggag cggctgcgcc aggctgcggc
     4201 acgtatcgcc ggcgccatcg cggcggggca ccgagccttc gagttcctgc aagggcactt
     4261 cctgcccatc gaggccgcgc ggggccacga ggaccgccca ctggtgttca cgccgatcac
     4321 gctgcccgat gccgcccacc tgcggccggg cgacgtgctg gtcatgagtc accagtacgg
     4381 tgtgctcccc gagtgtgtcg acatggcatt cgccgccagg cgccatggag cctacatcgt
     4441 ggccatgtgc ccgcccagcg acccagcggt gatcgtccgg tcacacccat caggcaagtc
     4501 agtgcccgac ctggccgacc tggtcatcag cacgcacatc cccgcggggg acgtcgccct
     4561 ccaggtcccc gccggggggc cgggagcctg tcccacgtcg ggaacgatcc aggccgcgat
     4621 gtactgggca ctggtctgcg cggtggcaga acgactgaga ccatagcgaa gcaggtgccg
     4681 ggcgggcgtc taccgcccgg catatacttc ggtggccata tgagcatcta ggctgaccag
     4741 ccgagaggct agccgcgagc cgcccacacc gttccccgct gcagcagcgt cttgaagtag
     4801 ctgttctggc acgccgggcc gtcatggccc agtgcgaggt agaacacccg gccgctaccc
     4861 cacggcttgg tccatgccac ggggcgggcg attccct
//
LOCUS       DAOSPL010000114         4885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_17430_length_4885_cov_3.839337, whole genome shotgun sequence.
ACCESSION   DAOSPL010000114 DAOSPL010000000
VERSION     DAOSPL010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17430_length_4885_cov_3.839337"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="PLQ54_03275"
     CDS             complement(<1..1255)
                     /locus_tag="PLQ54_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92308.1"
                     /translation="MAQGEPSRVIELTPAHGPGDAGRQEYDLINDGLVPLCVVGMLWA
                     FSRFALDVRSVVVAGDAVVVRFVLFWFLVGVVGIAKIEALRGGSALALPYKLGLATAT
                     GFFILRYSVTDVLLGDAGGGLGASVLLNSAVFAAVWWGATRITRDCTVFAGTEEQYSQ
                     GLLDEAMARAEPGDGLLPRRARPGKSVFVFSAVALPVFGLGQLVLARGDLGLYRSSYY
                     SVGAYVFFALLLLALTNLSGLKLYLAGKGLRAPRKLVSMWAASALVLTVLVMCLAVLM
                     PRRDLRSEDTWIARGLRVSEFNAPQPRMAPVSGTAQPRAAPRVWRADEHSRAQRSAHG
                     SPRRGESGSEGRSERGESAGPEPDGAANGGGQGAEPGAGGQEGEGEGAGAGRGEGNQP
                     SQPSAPEPPPLPNELMTVLEWLLIAL"
     gene            complement(1320..4661)
                     /locus_tag="PLQ54_03280"
     CDS             complement(1320..4661)
                     /locus_tag="PLQ54_03280"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOS92309.1"
                     /translation="MLRRMSTYVVPLVLLTGAMGGAAVAEAPASALADLRARFCSPEP
                     PRSIGPLYWYHGEDDADILEGIDKMHEGGMGNFTIESRPHSDYLGPKWWHDIEISLDR
                     AKQLGMQVYIFDEKWFPSGIAGGLVQATSPRFWRHWLQETTVPVDGPQADFRCDVPEG
                     EQVAGAVAVRRGGEPSEMLDLRERVSRDGRSLAWPAPAGQWDVFVYTMHSDAGHHDVL
                     NPEATARFIELTHEETYRRFKDYFGTTLVGFFMDEPGFYNGGGQWPWTYDLAAEFRAT
                     KGYDLCAFLPALTHPEWASYRWVRYDYMDVLTRRYAAAFYKPIQDWCRAHGVVSMGHL
                     IEHESTHQGLGAGPGHALRMMKHLDMGGIDLVFHQVMPGSRNTDYWGMPKLASSPSHL
                     YGMRNDLAMNETYGASGWTTGLPTMKWLADWQMVRGINFLIPHAFNPTYPDPDCPPHF
                     WAKGNDPQWMLFRHWADYTNRVCALLTGGRHVAPAAVLYTAESSWAGHADLIELVEAE
                     LLKAQYDFDLLDFDLFLDTDACRIRDGALRIAQEQFPLLVLPGVDAIPVAVMERIARF
                     VGNGGTLLSTCRLPQHSCERGQDARVQALVEQVFGPATKPGTLRERRHGSGGRAVFVP
                     ALGGVARALEGLGRPSDCTAYPRFEDLRYIHRVKDGADVFFLTNESIDETFSGTVSLA
                     ARGMPEIWDALAGTIEPAKVYSDHEGRTRIPLTLAPYESALIVFGSAQPSAHVIDTNL
                     VEVLHLDVASGGATVTGIARGASRPYATALAGGHYWHARNDRVNPLPPIELPAEGWTI
                     TRAGKTVTGALGDWSREDPHFSGSAKYTCTFLLPHDAAGPEEVELDLGAVGEIARVWI
                     NDQLAAELICPPYRARVTRWVRDGENRLTVEVANTLANYRVSQGEPPVYARVLPSGLM
                     GPVRIARMPVVTLELAPVRGPVIEPRIPGRRFASRSSAGNYAHVLNGGTVEASSTLSG
                     NFPAESVIDGDRTGSGWEQGTGGWNDATNGQWGDWIVVAFDGEKWIDRVEVTTLQDSY
                     RGADEPSAEARFTRYGIVDFDVEAEVGNEWRVVGSVRGNDLVRRTVRFAPLRTQRIRV
                     VVHRGLEGYSRIVEIGAYGPQTRGR"
     gene            complement(4669..>4885)
                     /locus_tag="PLQ54_03285"
     CDS             complement(4669..>4885)
                     /locus_tag="PLQ54_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HOS92310.1"
                     /translation="TTQMGKYDFSSWTIHMAFIIVFSNLWGIQFREWRGAGRKTVTLV
                     WAGIGILVLSTIVVGYGNYLGSLAKGA"
BASE COUNT          833 a         1794 c         1542 g          716 t
ORIGIN      
        1 ccagggcgat cagcagccac tcgaggaccg tcatgagctc gttgggcagc ggcgggggct
       61 ccggagctga cggctgcgac ggctggttcc cctcgcctcg tcccgcgccc gcgccttcgc
      121 cctcaccctc ctgccctccc gcgcccggct ccgctccttg gcctccgccg ttggcggcac
      181 cgtcgggctc ggggccggca gactcgcctc tctccgaccg accctcactg ccggattcgc
      241 cccggcgggg cgacccgtgt gcgcttcgct gcgcccgcga gtgctcgtcg gcacgccaca
      301 cccggggcgc tgcacggggc tgagccgtcc ccgacaccgg cgccatccgg ggctgcggcg
      361 cgttgaactc ggacacccgc agcccgcggg cgatccaagt gtcctccgac cgcagatcgc
      421 gccggggcat gagcaccgcc agacacatca ccagcaccgt cagcaccagg gccgaagcgg
      481 cccacatgct cacgagcttc cggggagccc tcaggccctt gcccgcgagg tagagcttca
      541 ggcccgacag gtttgtgagc gcgagcaaca acagcgcgaa gaacacatag gcgcccaccg
      601 agtagtagct gctccggtac agccccaggt caccccgggc caacaccagc tgaccgagcc
      661 caaagacggg cagcgccacg gcggagaaca cgaacaccga cttgccgggc cgcgctcggc
      721 gcggcaacag gccatcgccc ggctcggcac gagccatggc ctcatcgagt aggccctgcg
      781 agtactgctc ctcggtgccc gcgaacaccg tacagtcacg tgtgatccgt gtcgcgcccc
      841 accacaccgc cgcaaagacc gccgagttga gcagcaccga ggcgcccaag ccgccgcccg
      901 catcgcccag cagcacatcg gtcaccgagt acctcaggat gaagaacccc gtggccgtgg
      961 ccagcccgag cttgtagggc agggccaagg ccgagccgcc gcgcagggcc tcgatcttcg
     1021 cgatccccac cacgcccacg aggaaccaga acagcacgaa acgcaccacg acggcatcgc
     1081 cggccaccac gacggagcga acgtccagtg cgaaccgcga gaaggcccac agcattccca
     1141 cgacacacag gggtaccagg ccgtcgttga tgaggtcata ctcctgccgg cccgcatccc
     1201 ccggaccgtg cgctggggtc agttcgatca ccctcgaggg ctcaccctga gccacgacac
     1261 acctcgctca ccgggggcgg gcccgattcg cgcccgaggc agtggcccga acgcccgagt
     1321 caccggcccc gtgtctgggg cccgtaggcc ccaatctcga cgatccggct gtagccctcc
     1381 agacctcgat gcacgaccac ccggatgcgt tgggtccgca agggcgcgaa gcgcaccgta
     1441 cgtcgcacca ggtcattgcc gcggaccgag cccaccactc gccactcgtt gcccacctcc
     1501 gcctcgacat cgaagtccac gatgccatac cgcgtgaacc gtgcctccgc ggagggctca
     1561 tcggctcccc ggtaggagtc ctgcagggtg gtcacctcga cccgatcgat ccacttctcg
     1621 ccatcgaagg ccacgacgat ccagtcgccc cactggccgt tggtcgcgtc gttccagccg
     1681 ccggtcccct gctcccatcc ggaccccgtg cggtcgccgt cgatcactga ttcggccggg
     1741 aagttgccgc tgagtgtcga agatgcctcc accgtgccgc cattgagcac atgggcgtag
     1801 ttgcccgccg agcttcggct cgcgaaccgc cggccgggaa tcctgggctc gatgacgggg
     1861 ccccgcaccg gagccagctc aagcgttacc accggcatcc gggcgatgcg caccggaccc
     1921 atgagcccgc tgggaagcac ccgggcatac acgggcggct cgccctggct cacccggtag
     1981 ttggcgagcg tgttggccac ttcgacggtc aggcggttct cgccatcgcg cacccagcgg
     2041 gtcacgcgag cccgataggg cgggcagatc agctccgccg ccagctggtc gttgatccag
     2101 acccgggcga tctcacccac cgcgcccaga tccagctcga cctcctcggg gccggccgcg
     2161 tcgtgtggca gcaggaaagt gcacgtgtac ttcgccgatc cagagaagtg gggatcctcg
     2221 cgtgaccagt ctcccaacgc cccggtcacc gtcttccccg cccgcgtgat ggtccacccc
     2281 tccgctggca actcgatggg cggtaacggg ttcacgcggt cgttccgagc gtgccagtag
     2341 tggccgccgg ccagcgcggt agcgtacggc cgactcgcgc cgcgggcgat gcccgtcacc
     2401 gttgccccgc cgctggccac atccaaatgg agcacctcga ccaggttcgt atcgatgaca
     2461 tgagcggagg gctgggcgga cccgaacacg atcagcgccg actcgtatgg cgccagcgtc
     2521 aaggggatgc gggtacggcc ctcgtggtcg ctgtagacct tcgcaggctc gatcgtccca
     2581 gccaaggcat cccatatctc gggcatgccg cgggccgcga ggctgacggt accgctgaag
     2641 gtctcatcga tcgactcgtt ggtcaggaag aacacgtccg cgccgtcctt gacgcggtgg
     2701 atgtagcgca ggtcctcgaa gcgtgggtag gccgtgcagt cactgggtcg ccccagaccc
     2761 tcgagcgccc gggccacacc gcccagcgcc ggcacgaaca cagcccggcc tcccgagccg
     2821 tgccggcgct cgcgcaacgt gcccggcttc gtcgccggcc cgaaaacctg ctccaccagc
     2881 gcctgcaccc gagcgtcctg cccccgctcg cacgagtgct gcggaaggcg gcaagtggac
     2941 agcagagtcc caccgttgcc gacgaagcgc gcgatccgct ccatcacggc caccggtatc
     3001 gcgtccaccc cgggcagcac cagcagcggg aactgctcct gcgcgatccg gagagcgcca
     3061 tcgcgaatcc ggcaggcatc ggtgtcgagg aacaggtcga agtccagcag gtcgaagtca
     3121 tactgagcct tgaggagctc ggcctcgacc agctcgatca gatccgcgtg gccggcccaa
     3181 ctcgactcgg ccgtgtacag caccgccgcc ggcgccacat ggcggccgcc tgttagcaga
     3241 gcgcacacac ggttcgtgta atccgcccaa tggcggaaca gcatccactg ggggtcgttc
     3301 cccttggccc agaagtgcgg ggggcagtcg ggatcggggt aggtcgggtt gaacgcatgg
     3361 ggtatgagga agttgatccc gcgcaccatc tgccaatcgg ccaaccactt catggtcgga
     3421 aggcccgtcg tccagcccga ggccccatag gtctcgttca tggccagatc gttcctcatc
     3481 ccgtacagat gcgagggcga cgaggccagc ttgggcatgc cccagtagtc ggtgtttcgc
     3541 gacccgggca tcacctggtg gaagaccagg tcgatccccc ccatgtcgag gtgcttcatc
     3601 atgcgcagcg cgtggccggg gcccgccccc aaaccctggt gcgtcgactc gtgctcgatg
     3661 aggtggccca tcgagacgac gccgtgcgca cggcaccagt cctggatggg cttgtagaac
     3721 gccgcggcat agcgccgtgt caggacatcc atgtagtcgt agcgcaccca gcggtacgag
     3781 gcccactcgg gatgggtcag cgcggggagg aaggcgcaga ggtcgtaccc cttggtggcg
     3841 cggaactcgg cggccagatc gtacgtccac ggccactgac cgcctccgtt gtagaacccg
     3901 ggctcatcca tgaagaagcc gaccagcgtc gtgccgaagt agtccttgaa ccgccggtag
     3961 gtctcctcat gggtcagttc gatgaaccga gccgtggcct caggattcag cacgtcgtgg
     4021 tgcccggcat cggagtgcat cgtgtagacg aacacatccc actgccccgc cggggccggc
     4081 cacgccagcg accgaccgtc gcgcgagacg cgctcgcgca ggtccagcat ctcggagggt
     4141 tccccgcctc ggcgcacggc gacggcaccg gccacctgct cgccctcggg gacgtcgcaa
     4201 cggaagtcgg cctggggccc atcaaccggt accgtggtct cctggagcca gtgccgccag
     4261 aacctggggc tcgtcgcctg caccagcccg ccggcgatgc cactggggaa ccacttctcg
     4321 tcgaagatgt acacctgcat gcccagctgc ttggcccggt cgagtgatat ctcgatgtcg
     4381 tgccaccact tggggcccag gtagtccgag tgcggccggc tctcgatggt gaagttgccc
     4441 atcccgccct cgtgcatctt gtcgataccc tcgaggatgt cggcgtcgtc ctcgccgtgg
     4501 taccagtaga gcggcccgat ggaccgggga ggctccggcg agcagaaccg agctcgcaga
     4561 tcagccaggg ccgaagcagg cgcctcagcc acggccgcgc cccccatggc acccgtgagc
     4621 agcacgagcg gcacgacgta cgttgacatt cgtctcagca tgcccgggct acgctccctt
     4681 cgccaatgac cccaagtagt tgccgtaccc gaccacgatg gtcgagagca cgagaatgcc
     4741 aatcccggcc cacacgaggg tcaccgtctt tcgccccgcc cctcgccact cgcggaactg
     4801 aatcccccac aggttgctga agacgatgat gaacgccatg tggatggtcc aactggagaa
     4861 gtcgtacttt cccatctggg tggtg
//
LOCUS       DAOSPL010000115         4880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_17455_length_4880_cov_2.696788, whole genome shotgun sequence.
ACCESSION   DAOSPL010000115 DAOSPL010000000
VERSION     DAOSPL010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17455_length_4880_cov_2.696788"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PLQ54_03290"
     CDS             complement(<1..710)
                     /locus_tag="PLQ54_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92311.1"
                     /translation="MAPMLEGFAGQEVALRLVANALQRDEVPHAYLVAGPRWLGKRRA
                     ALSLGRLLACHAPRLESTDGPTPCGECRSCVLPFGPGTHHDLVVIEPEAPPAKTAATD
                     EASGEASAESVIAVASGVIRVEQIRDAERRLSRSLVAGRRRVCVVPDAERMCRVASAG
                     NAFLKTLEEPPPHTAIVLTASNPADLLPTIVSRCLMLPFHPLSAEQLARAMCWVEGGA
                     GVAESEAALAAALSCGRLG"
     gene            complement(694..1344)
                     /gene="tmk"
                     /locus_tag="PLQ54_03295"
     CDS             complement(694..1344)
                     /gene="tmk"
                     /locus_tag="PLQ54_03295"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOS92312.1"
                     /translation="MRPAGRGLFVAFEGGDGTGKTTQCRLLVDALRARGVAVVATREP
                     GGTALGDRVRDLLLHSGIDIGPMAEALLFAAARAQHVTEVIAPALARGETVVCDRFLG
                     SSLVYQGIGLGLGMETVRAVNELATAGVLPDVTVVLDVPVEESLRRKHADGELPDTVE
                     SRGSRFHERVREGYRGLGSQLPGRVAVLDGTGPVGEVHGRVCAIIDAALRGYGTDA"
     gene            complement(1341..2852)
                     /locus_tag="PLQ54_03300"
     CDS             complement(1341..2852)
                     /locus_tag="PLQ54_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOS92313.1"
                     /translation="MSALPSDLQESLRGDPRGDALLEVVMDLGRLPEARYPDRIVALR
                     ATPVTEDDLRYVASSVSAFGGDNRAGIERSLHRISAIRNRGGQIVGITCRVGRAVYGT
                     IDIIQDIVETGKSILLLGRPGIGKTTKLREIARVLADTVNRRVVVVDTSNEIGGDGDI
                     PHSAIGRARRMQVPVPAAQHAVMIEAVENHMPEVIIVDEIGTEEEARAARTIAERGVQ
                     LVATAHGNTLENVLMNPTLSDLVGGVQAVTLGDDEARRRGTQKTVLERRAPPTFDIVI
                     EMVEMDELVVHEDVAQAVDALLRGMEPPRECRRRAADGSVERERVVATSEPTMLKGTA
                     IAGRWADATPVAPDEGRRTAHVYAYGISRDRLDQAIRELGVAARVTKDWKEADLVVSL
                     RAHQKRFRAKLRTLGPDTAVRFLRGNTYAQVYGCLREFFSLSRESTEEQAIEEARTAI
                     ERAIAGESDIELTPRNSYLRKLQHELASAYQIESRSVGEEPRRRVVIAGGSGR"
     assembly_gap    2854..2863
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3182..4570
                     /locus_tag="PLQ54_03305"
     CDS             3182..4570
                     /locus_tag="PLQ54_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92314.1"
                     /translation="MRPLALSVAFMVSIVIPIAWGALTGPEPERLAGAEPAALQITDT
                     RAVAAIPDLMAEGIFRQIEYLYDISFSPDGNWLAFVLDAIPDKARMHRYYVYLLQRTG
                     QNRSLMLTTKAGPALSRSFVWLGDHTPLLCSPEFKTPANAAPEQPGPTGASIQIRGTD
                     PKLSPDGGRVVFRQGDEEDASLWLADLQRLEPRQLAASASPFGDFAWSPDGSRVAFLA
                     PSKTVDGDSLWWVVPDESPSEALSVSITRFAWSPNGDLLAALSDAGEIHVIEVGGGSW
                     PVAEAARDFSWSPDGQLLAYISEAPTGTAEAVHVRSLVTGKDWIAMQGKAAEGWDFGA
                     VHFSPDGGRIYARAAAGRDVSGDGQYFDKSDRVMLWASTEPGATAAEVPIAGSVQQPA
                     VAPGGRLMAVTVEYAGKSSLWCIDLENGQVASMGDVPVRKYAYGLAWASDGANLAFED
                     QGNLMLSTVARK"
BASE COUNT          802 a         1862 c         1453 g          753 t
ORIGIN      
        1 cccagccgcc cgcacgaaag ggccgccgcc agcgccgcct cgctctccgc cacccctgcg
       61 ccaccctcga cccaacacat cgcccgcgcc agttgttccg cgctcagggg atggaatggc
      121 agcatgaggc agcgggagac gatcgtcggt agcaggtccg ccgggttgct tgcggtaagc
      181 acaatcgccg tatggggagg aggttcctcc agagtcttca ggaaagcatt ccccgcggac
      241 gccacccggc acatccgctc cgcgtccggc accacacaca cccgacgcct cccggccacc
      301 aggctgcggc tcaaccggcg ttcggcatcg cggatctgct ccacacggat cacgcccgag
      361 gccaccgcga tgaccgactc ggcgctggcc tcgccactgg cctcgtccgt ggccgccgtc
      421 ttcgcaggag gggcctccgg ctcgatgacc accagatcat ggtgcgtccc cgggccgaac
      481 ggcaggacgc acgagcggca ctcgccgcaa ggggtcggac cgtcggtgga ctcaagccga
      541 ggagcgtggc acgccaacag gcggccgagg ctcagcgccg cccgccgctt gcccagccac
      601 cgaggtcccg ccacaaggta cgcatggggc acctcgtcgc gctgcagggc gttggcaacc
      661 agccgaagcg caacctcctg cccggcgaac ccctcaagca tcggtgccat agcccctcag
      721 cgccgcgtcg atgatcgcgc atacccgccc gtgcacctcg ccgacgggcc cggtgccatc
      781 cagcacggcc acgcggcccg gcaactgact gcccaggccc cggtacccct cgcgcacccg
      841 ttcgtgaaac cggctgccgc gcgactccac cgtgtccggc agctccccgt ccgcatgttt
      901 gcgtcgaaga ctctcctcga cagggacatc gagaaccacc gtgacatccg gcagcacccc
      961 agccgtggcc agctcgttca cggcgcgtac cgtctccata ccgagcccca gcccaatgcc
     1021 ctgatagacc aggctcgacc ccagaaaccg atcgcacacc accgtctcgc cccgcgccag
     1081 ggcgggcgca atcacctcgg tcacgtgctg ggcgcgggcc gccgcgaaga gcagggcctc
     1141 cgccatggga ccgatgtcga tccccgaatg caaaagaaga tcccgaaccc gatccccgag
     1201 ggctgtgccc ccgggttccc tcgtcgcaac gacggcgaca cctcgagcgc gtaacgcatc
     1261 cacgagcaga cggcactggg tcgtcttgcc cgtcccgtcc cctccctcga aagccacgaa
     1321 cagaccccgg cccgccggcc tcatcgcccg ctcccgcccg cgatcaccac gcgacggcgc
     1381 ggctcctcgc ccacactccg cgactcgatc tggtaggcgc tggccaactc gtgctggagc
     1441 ttcctcaaat acgagttccg tggggtcagc tcgatgtcgc tctctcccgc gatggcccgc
     1501 tcgatggccg tccgggcctc ctcgatggcc tgctcctccg tgctctccct cgacagcgag
     1561 aagaactcac gcaggcatcc atacacctgg gcgtaggtgt tgccccgtaa gaagcgcacc
     1621 gccgtgtccg gcccaagcgt ccgcagtttc gcccggaaac gcttctgatg ggcccgcagc
     1681 gacacgacca ggtccgcctc cttccaatcc ttggtaaccc gagccgccac tcccagctcc
     1741 cggatcgcct ggtccagccg gtcccggctg ataccatacg catacacatg cgccgtccgg
     1801 cggccctcat ccggagccac cggcgtcgcg tcggcccacc ggcccgcaat cgccgtgccc
     1861 ttcagcatcg tgggctccga agtggccaca acgcgctcgc gctccaccga cccatctgcg
     1921 gcccgcctgc ggcactccct cggcggttcc atgccccgca gaagggcgtc caccgcctga
     1981 gccacatcct cgtgcaccac cagctcgtcc atctcgacca tctcgatgac gatgtcgaag
     2041 gtcggcggcg cccgccgctc caacaccgtc ttctgggtgc cgcgccgacg cgcctcgtca
     2101 tcacccaggg tcaccgcctg aacaccaccg accagatcag aaagtgtcgg gttcatcagc
     2161 acgttctcga gcgtgttgcc atgcgccgtg gccaccaact ggacaccgcg ctccgcaatc
     2221 gtccgggccg cccgtgcctc ctcctcggtc ccgatctcat ccacgataat gacctccggc
     2281 atgtggttct ccaccgcctc gatcatcacc gcatgttgcg ccgccggcac cggaacctgc
     2341 atgcggcgcg cccggccaat ggcgctgtga gggatgtccc catccccacc aatctcattc
     2401 gaagtatcga ccaccacgac tcggcgattc accgtgtccg ccagaacccg ggcgatctca
     2461 cgcagtttcg tcgtcttccc gataccaggc cgacccagta gcaggatgga cttgccggtc
     2521 tcaacgatgt cctggatgat atcgatggtg ccgtacaccg ctcgcccgac gcggcaggtg
     2581 atcccgacga tctggccgcc tcggttgcgg atggccgaga tgcggtgaag gctgcgctca
     2641 atgcccgccc gattgtcgcc gccaaaggcg ctcacgctgg acgccacata ccgcaggtcg
     2701 tcctccgtca ctggcgtcgc gcgcagcgct acgatacgat ccggatagcg cgcctcgggg
     2761 agtcgtccca gatccatgac cacctcgagc aatgcgtcgc ccctcggatc gccccggagg
     2821 gactcctgca ggtcactcgg aagagcgctg atgnnnnnnn nnnggtcgct ctcgatcgtc
     2881 aggccggatc gcaagtccat gttgaatacc ctatcgcctc gtctcgccgc aggcccaatg
     2941 aacgagggga tgaatcgcgt agcggatcag cgggatcggg aggggcgggc ccaggatatg
     3001 cgggcgccgg caggtgagcc gagaagccaa ccgaaggtcg atgccatcca gccgtaagac
     3061 cacgtcgagg gccgcttctc atccaaccgc agtataaaag cgccccggtc gggtgtcaag
     3121 gaaacgcccg gccagctcgt gcgtccaaat gccaaccctc gaggacttga gaggagaccc
     3181 tatgcgaccc ctagcactgt ctgtcgcatt catggtgtcc atcgtcatac cgatcgcctg
     3241 gggcgcgctg accgggcccg aacccgaacg gctggccggg gccgaaccgg cggcattgca
     3301 gatcaccgac acacgcgctg tggccgccat ccccgatctc atggccgaag ggatcttccg
     3361 acagatcgag tacctctacg acatctcatt ctcgcccgac ggcaactggt tggcattcgt
     3421 gttggacgcc atacccgaca aagcgcgcat gcaccgctac tacgtctacc tcctgcagcg
     3481 aacgggtcag aaccgatccc tcatgctgac caccaaggcc ggacccgcgc tgtcgcgctc
     3541 attcgtctgg ctgggcgacc acactccgct cctgtgctcc cccgagttca agacccccgc
     3601 caacgccgcc ccggagcaac cgggccccac gggtgcatcg atccagatcc gtggcacgga
     3661 ccccaagttg tcgcccgatg gtggccgagt ggtctttcgc cagggcgacg aggaggatgc
     3721 ctccctgtgg ttggcggatc tccagagact cgagccccgc cagctcgccg ccagcgcctc
     3781 gcccttcggc gatttcgcgt ggtcgcccga cggcagtcgc gtggccttcc tcgccccctc
     3841 caagaccgtt gacggcgaca gcctgtggtg ggtggtcccc gatgagtctc cttccgaggc
     3901 gctctccgtc tccatcacgc ggttcgcttg gtcgcccaac ggcgatctgc tcgcagctct
     3961 atccgacgcg ggtgagattc acgtcataga ggtgggtggc gggtcctggc ccgtggctga
     4021 ggcggctcgc gacttctcgt ggtcgcccga cggccagctc ctggcctaca tctccgaggc
     4081 gcccacgggc accgccgagg cggttcacgt ccgctcactg gtcacaggca aggactggat
     4141 cgccatgcag ggcaaggcgg ccgagggatg ggacttcggc gcggtccact tctcgcccga
     4201 tggcggccgc atctacgcgc gcgccgccgc aggacgagac gtcagcggtg acggccagta
     4261 cttcgacaag tcggaccgcg tcatgctgtg ggcctcgacc gagcccgggg ccaccgccgc
     4321 cgaggtcccc atcgccggat ccgtgcagca acctgccgtc gcccccggcg gccgtctcat
     4381 ggccgtgacc gtcgagtacg ccggcaagtc tagcctgtgg tgcatcgacc tcgagaacgg
     4441 acaggtcgcg agcatgggcg acgttccggt ccggaaatac gcctacggcc tcgcctgggc
     4501 ctcagacggt gccaacctgg ctttcgagga ccagggtaac ctcatgctct caaccgtggc
     4561 acggaagtag gcgatctcgg ccggcccgac ggcctgctct cgtgccgggc gcaagggcga
     4621 ccggcatgtc gccccagagc ccaccacccg aagaccccca accccgccgt tcccgagccg
     4681 tcgcgcccgc ccggcgtgac gaagggcgcc gcgacctcgc cccatcccgc aggccccgct
     4741 tcgcgggccc cttccatggt ggttcgtacg gtcttcggag gggcccccaa tgagcccggc
     4801 tggccgcata gggcgaccgt ctcggtcgcc catcaggtgt cgagcgggac gatcgggtct
     4861 caccggcctc ctgctgtctt
//
LOCUS       DAOSPL010000116         4868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_17500_length_4868_cov_3.293995, whole genome shotgun sequence.
ACCESSION   DAOSPL010000116 DAOSPL010000000
VERSION     DAOSPL010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17500_length_4868_cov_3.293995"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PLQ54_03310"
     CDS             complement(<1..936)
                     /locus_tag="PLQ54_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92315.1"
                     /translation="MAGCLRCGVPALLLFALMTSAVRAAPGASAGPPPYTFRLLAERH
                     LRSLDALPDMDADGYGELAWGVSYGMMALNVLYEATGDTRFLARQADLVERVLGKRDS
                     NLAAAHGSEAYVDYQRGRVLRSWGTSHYSAGRHTCWLVHAGMLLYPMAEYVRLVQAGG
                     DRTRPWLDRARRVVPAVEETIAEFDAEWRDGPQPGQGYYVFPSGDLAPNNQMNAPGRA
                     LFVLADVTGKTEYRDRARKLAAYFRSKLTHVADGDYYIWAYSQQTPDGPPGRGEDVSH
                     AAINAHFAYVAWEHGASFTDTDMARLARTLTHGLYL"
     gene            complement(949..1911)
                     /locus_tag="PLQ54_03315"
     CDS             complement(949..1911)
                     /locus_tag="PLQ54_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92316.1"
                     /translation="MTIRRVWSVAGAALLALTVLIGAAPQARAYNGNIMGMMWDCEKA
                     QASGNWSRAKKLVSIALLHLKDEGFTRTERALEKLSELPPDADEAQRRFADVEKAFLT
                     LRLEWLTSLRDENLWWKDELAKPDAGVLGASRQEILRKMAAVAACLTPECEATRLELK
                     SADFEKAHALRLQALGLWQAEVEESANRLRRAENEATGEGAEAAQAGARSEYLRRWLS
                     KATRYSDALGKEVAAEVDPAFLVGRMKYYEAKCQLGRRIKDEAFGSPIVEMYRQKAAW
                     AAAKAGVEVPADAEIRAPEWYCASVMEPSWFQFKWALPVVADAG"
     gene            complement(2108..3184)
                     /locus_tag="PLQ54_03320"
     CDS             complement(2108..3184)
                     /locus_tag="PLQ54_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009593018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOS92317.1"
                     /translation="MGGITAATASAIGGMALLLPAARGPEYVPPRGYVCYRAPGPVDI
                     DGRLGKAVWAAAPWTEDFGDIEGDVKPPPPLRTRAKMLWDDEFFYVGAELEEPHVWAT
                     LTEHDSVIFHDNDFEIFIDPDGDSHEYYEIELNALGTEWDLRLPKPYRDGGSAVNEWE
                     IPGLRLAVHVEGTLNDPSDIDRFWSVEIAFPWSALGGFAGVPCPPRHGDQWRINFSRV
                     EWQTTVQDGRYVKVPGRPEDNWVWSPQHVVDMHRPETWGYVQFSTAEPGRDAFVPDPS
                     GPGRWVLHRIYYAQQDFREANGVWARTLGDLGLEGLTHDSIAEGPELRQTAAGWEADV
                     RIGAEVGAAVWRIREDSRIWRPKV"
     gene            3287..>4868
                     /locus_tag="PLQ54_03325"
     CDS             3287..>4868
                     /locus_tag="PLQ54_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92318.1"
                     /translation="MSGPATPHQLDGPRRARPTRRAVVMGLALVAWTAWLTPYNDSVL
                     SNTFLAGTSFPMAAFGILVLLAVPLNALLHRVRAGIPPFSAAELITIWALMMVASGIP
                     GSGLLRMMVPQAAGYRYGATPENKWEERLGPHLRPYMVVDDKEAATGFFTGVSDAPPI
                     PWIPSDWSIANWNAIPWGAWLRPAMGYGVLTLGVFLGYICLASIVRRQWVERERYPYP
                     LADLPMELALESERGRGIPALFRNGPFLATTILITGLHGYAGLCRLHPAFGGFRFALD
                     LQSVFTEVPWRYLPGALKYWRIHPIGIGLTYAVPTDVLFSVWFMKVFIGLQEMVYGLR
                     GDYGGEVAFGWDPAYQCYPQLAAYLALAGWILFTGRHHFRQVFRSAFVGTGMDDVDEP
                     MPYRWAARGFLLAIGILMGWFLYSGVRFFVAATVVIVFFAIALGCSWLVCQAGQMVVA
                     ARTVPSDTLVGLFGKVWQPRYFGRQWWRIEMINAKELAVVPLFESPFSKDLREILMPS
                     VANLTRAADVGVCRRALLG"
BASE COUNT          751 a         1720 c         1545 g          852 t
ORIGIN      
        1 gaggtagagc ccgtgtgtca gcgtgcgcgc caggcgggcc atgtcggtgt cggtgaacga
       61 ggcaccatgc tcccacgcca cgtaggcgaa gtgcgcgttg atggccgcgt gggagacatc
      121 ctccccccgg ccagggggac catctggtgt ctgctgtgaa taggcccaga tgtagtagtc
      181 gccgtcggcg acgtgagtga gcttgctacg gaaataggcg gcaagcttcc gtgcccgatc
      241 ccggtattcc gtcttgcccg tcacgtccgc caacacgaag agcgcacggc ccggggcgtt
      301 catctggttg ttcggggcaa ggtcgccgct ggggaacacg tagtagccct ggccgggttg
      361 cggcccgtcg cgccattcgg cgtcgaactc ggcaatggtc tcctccacgg cgggcaccac
      421 gcgccgggcg cggtccagcc acggccgtgt tcggtcaccg ccggcctgga ccaggcgcac
      481 atactcggcc atgggataga gcagcatccc ggcgtggacc agccagcagg tgtgccggcc
      541 tgcactatag tggctcgtgc cccacgagcg gagaacgcgg ccgcgctggt aatcgacata
      601 ggcctccgaa ccgtgtgcag ccgccaggtt ggagtcccgc ttgccgagca ctctttcgac
      661 caggtcggcc tggcgcgcca ggaagcgtgt gtcgcccgtg gcctcgtaga gcacattgag
      721 cgccatcatc ccataggaga cgccccatgc gagctcgcca tagccgtcgg cgtccatgtc
      781 cggcagggca tcgagactcc gaagatgacg ctcagccaag aggcggaacg tgtacggagg
      841 agggcccgct gacgcgccgg gggctgcgcg caccgcggac gtcatcagcg cgaacagcaa
      901 cagggcgggg acgccgcatc ggagacaacc agccactggg tcgcgcccct atccagcatc
      961 cgccacgacc ggcagtgccc acttgaactg gaaccagctt ggctccatca cggacgcgca
     1021 gtaccactcg ggcgcgcgga tctccgcatc agccgggact tccacaccgg ccttggctgc
     1081 ggcccaggcc gccttctgcc ggtacatctc gacgatcggc gagccaaatg cctcatcctt
     1141 gatgcgccgc ccgagctgac acttggcctc gtagtacttc atgcggccga ccaggaaggc
     1201 tggatccacc tcggccgcaa cctccttgcc cagagcatcc gaatagcgcg tcgctttgct
     1261 gagccatcgc cgcagatact cggaacgcgc ccccgcctgc gccgcctcag caccctcacc
     1321 ggtagcctcg ttctcggcac gccgcaggcg gttggcgctc tcttcgacct ctgcctgcca
     1381 caaaccgagt gcctgcagac ggagcgcatg ggccttctcg aagtcggccg acttgagctc
     1441 cagcctggtg gcctcgcact ccggagtgag acaagcggca acggccgcca tcttccgcag
     1501 gatctcctgc cggctggcac ccagaacacc ggcatccggc ttggccagct cgtccttcca
     1561 ccacaggttc tcatcccgca gcgaggtcag ccattcgagc cgcaacgtga ggaacgcctt
     1621 ctcgacgtct gcgaaccgcc tctgggcctc atcggcatcc gggggcagct cagacagctt
     1681 ctccagggct cgctcggtcc gcgtgaaccc ctcgtccttg aggtgaagca gggcgatact
     1741 gaccagcttc ttcgcccggc tccaattgcc gctcgcctga gctttctcgc agtcccacat
     1801 catgcccatg atgttgccat tgtaggcgcg cgcctgcggg gcggcgccga tcagcaccgt
     1861 gagggcgagg agcgccgctc ccgccaccga ccatacccgt cgaatcgtca ctggtcgtgt
     1921 ctccttgtca tcgtggggta cgctgtccgc cttcgcggcg ggtggcaccc gagtcgtgca
     1981 ctgggacggg ctctcgatcc cgggctcccg agcccgggga gcatggtagc cgggtccagc
     2041 ccgggccggc aatagcgagc catgctgcct gcagcctgtg ccgcactccg ctagagccgc
     2101 cccatgctca gaccttgggg cgccagatcc tcgagtcctc acgaatccgc cagactgcgg
     2161 ccccaacttc ggcgccaatg cgcacgtccg cttcccatcc ggcagccgtt tggcggagtt
     2221 cgggaccctc agcgatgctg tcatgtgtca gcccctccag acccagatcg cccaatgtgc
     2281 gagcccagac gccgttggcc tcgcggaagt cctgctgggc atagtagatc cggtggagca
     2341 cccatcgccc tggacctgat ggatccggca cgaaggcgtc ccgacccggc tccgcagtcg
     2401 agaactggac gtacccccac gtctccggcc ggtgcatgtc aaccacatgc tgcggtgacc
     2461 acacccagtt gtcttcgggc cggcccggca ccttcacata ccggccatcc tgcaccgtag
     2521 tctgccactc aacgcgcgag aagttgatgc gccactggtc gccatggcgg ggagggcagg
     2581 ggacaccggc gaacccgccc agcgccgacc aggggaacgc gatctcgacc gaccagaacc
     2641 ggtcgatgtc ggacggatcg ttgagggtcc cttcgacatg cacggccagt ctcaggccgg
     2701 ggatctccca ctcgttgacc gccgaccctc cgtcgcggta cggcttgggc aaccggaggt
     2761 cccactcggt tcccagcgca ttcagctcga tctcgtagta ctcgtggctg tcaccatcgg
     2821 ggtcgatgaa gatctcgaag tcgttatcgt gaaagatgac cgaatcgtgc tccgtgagtg
     2881 tggcccagac gtgcggctcc tcgagctctg cccccacgta gaagaactcg tcatcccaca
     2941 gcattttcgc gcgcgtccgg agcggtggcg ggggtttgac gtccccctcg atgtcgccga
     3001 agtcctcggt ccacggtgcc gcggcccaca cggccttgcc caggcgcccg tcgatatcca
     3061 cgggaccagg tgcacgatag cagacgtaac cccgaggagg cacgtactct ggtccgcgcg
     3121 cggcgggcag cagcagtgcc atgcccccga tcgccgatgc agtggccgcg gtgatcccgc
     3181 ccatggtctc agcccccaag cgtggggagc tgcattcggt gcggcgtggc gcggcgcctt
     3241 cgacagccgg aggagtcggc ccgggacgag ccgaagcctc agggccgtga gcggtcccgc
     3301 caccccgcat cagctcgacg gtccgcggcg tgcacgtccc acgcgccgcg cggtcgtcat
     3361 gggcctggcg ctcgtggcgt ggacggcatg gctcacgccc tacaacgact cggtgctctc
     3421 gaacaccttc ctcgcgggta cgagtttccc catggccgcc ttcgggatcc tggtcctgct
     3481 ggccgtgcca ctcaacgctc tgctccaccg cgtgcgcgcc ggcatccccc cgttctctgc
     3541 ggccgagctc atcacgatct gggccctcat gatggtcgcg tccgggattc ccggttctgg
     3601 gctgctgcgg atgatggtgc cgcaggccgc cgggtaccgc tacggcgcga cgcccgagaa
     3661 caagtgggag gagcggcttg ggccccacct ccggccctac atggtcgtcg acgacaagga
     3721 ggcggccact ggtttcttca ctggcgtgtc cgatgcgccg ccgattccgt ggatcccgag
     3781 cgactggtcg atcgccaact ggaacgccat tccgtggggc gcgtggctcc gtccagccat
     3841 gggctacggt gtgctgacac tcggcgtctt cctcggctac atctgcctcg cgagcatcgt
     3901 ccgcagacaa tgggtcgagc gcgaacgcta cccgtaccca ctggccgacc taccgatgga
     3961 gctggccctg gagtccgaga gagggcgcgg aatccctgcc ctgttccgca acggcccttt
     4021 cctggcgacc accatcctca tcacaggatt gcacggctat gcgggcctgt gccgcctgca
     4081 ccccgccttc ggggggttcc gcttcgcgtt ggacctccag agtgtgttca ccgaggtccc
     4141 gtggcggtac ctgcccgggg cgctcaagta ctggcgcatc caccccatcg gcattggcct
     4201 gacgtacgcc gtgcccaccg atgtcctctt ctcggtctgg ttcatgaagg tgttcatcgg
     4261 cctgcaggag atggtctacg gactgcgcgg cgactacggc ggcgaggtgg ccttcggttg
     4321 ggacccggcc taccagtgtt acccgcagct cgccgcgtac ctcgctctgg ctggatggat
     4381 cctcttcacc ggacgccatc acttccgcca ggtcttccgc agtgctttcg tggggaccgg
     4441 aatggacgat gtcgacgagc ccatgcccta ccgctgggcg gcgcggggct tcctgttggc
     4501 catcggtatc ctcatgggct ggtttctcta ctcgggggtc cggttcttcg tcgcggccac
     4561 cgtcgtgatc gtcttcttcg ccatcgcgct cgggtgctcg tggctcgtct gccaggccgg
     4621 gcagatggtc gtggccgccc gcaccgtgcc gagcgacaca ctcgtcggcc tcttcggcaa
     4681 ggtctggcag ccccggtact tcgggcgcca gtggtggcga atcgagatga tcaacgcgaa
     4741 agagctcgcc gtcgtcccgc tgttcgagag cccattcagc aaggacctgc gcgagattct
     4801 catgccgagc gtcgccaacc tgactcgcgc ggccgatgtg ggagtctgcc ggagggccct
     4861 cctcggca
//
LOCUS       DAOSPL010000117         4857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_17540_length_4857_cov_3.264057, whole genome shotgun sequence.
ACCESSION   DAOSPL010000117 DAOSPL010000000
VERSION     DAOSPL010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17540_length_4857_cov_3.264057"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1548)
                     /gene="rho"
                     /locus_tag="PLQ54_03330"
     CDS             complement(<1..1548)
                     /gene="rho"
                     /locus_tag="PLQ54_03330"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOS92319.1"
                     /translation="MSASDLHSKTLPQLRALAKARGLEGITGLKKADLVARLLEAGVD
                     APAPGPVAPAEPARSEMPVSAPEAARPEAAAPAESTAPRRRGRSGRQGAPTGVRHDAE
                     ERDVEASRDLEPEPEPEQQHEPQGSIASTAPMPTAAPPAPTGFVVPSAPVTFGTPDPA
                     ESVPVPPMGAEVELPGVTMRDKPDRPDAAEEEPPRHTETVDRDRHEPRYEYTRGRLSG
                     RSRRGDRGGNGDSRHEAPRVDGRPDQRSGQRHDGRGDGRPQQDARPDRRGRGDSRDMR
                     EPRDGRGEAEGDARHLVTGILEILPEGRGFLRRAGYLPANDDVYVSESQIRRFGLRTG
                     DTVVGEARPPRDGTSERYYSLLRIESVNSAEPESCRNRPDFEDLTPIFPTERFVLETD
                     RNRLSNRLIDIISPIGKGQRGLIVAPPKAGKTTILKDIAHALITNNPEVVLLVLLVDE
                     RPEEVTDISRAVGSGGEVIASTFDQLPENHMRAADFCLERAKRLVEHGRDVTILLDSL
                     TRLARASK"
     gene            complement(2046..3239)
                     /locus_tag="PLQ54_03335"
     CDS             complement(2046..3239)
                     /locus_tag="PLQ54_03335"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOS92320.1"
                     /translation="MEWLARAVPWASPGTIPLLLAPLFLMGFGLNRADTAALLPDGTS
                     VAGVAVGGRTLEDARAAIAARAEQLVRGPVTLVCGSTARVTALASLGASFDVDGALKE
                     ASRDPGDSGPRDVPLSVRMDRTKLRQRLVAVGEAVRRAPRDATIRIAGIRAVIVPEVV
                     GVALDVDRAVTAVEKALTEGAVRRVIELPLVFTPPGRTRQALAGYRVLGHCATRFNAS
                     QANRTQNVRLAVSSVDDTWIPAGGVFSLNASTGQRSPQSGYLKANVYSGSRVVLGYGG
                     GVCQVSTTVYNAALMAGMPIVERHPHSRSVPYVPWGRDATVSWGTADMKFRNATDAAL
                     LVRSWVEGDSLHVCLVGRPKAPPVPAEPETALAGPTGGGSTTGAARPVNAAADRAVDA
                     YGSVE"
     gene            3548..>4857
                     /locus_tag="PLQ54_03340"
     CDS             3548..>4857
                     /locus_tag="PLQ54_03340"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOS92321.1"
                     /translation="MVRSSVTTVARLAWLVPSLVAFSAGLGWCQEAVAPPAGGAPAPD
                     TAPLGTPRAPVVPAAGTPAQQASLTTPLISVARTGVAPDIDGLRTEPLWSRAMRISHF
                     VRLDGAARPENQTEVFLTYDDSALYVAISCEEPQLETLSDRAKVRDSEEILRDDFVEL
                     LIAPSRTSPAYSRILVNPAGTVADLGGADGSVTWTCEGLRVGAQRGSLRWLVELAIPF
                     SALGATPTDGDVWGFNVARYRARDGEFSCYAGAGTGVGYRSPGNFATLVFAGSTIIVD
                     LVDIGDLFGELKGGAELKVRLRSTQPRRATPVARVIRGFDERRFGYSGVTVDVPALNP
                     ARIVIPYEVDDSRGAVIQLGVLDDAGATVFLSPKIPLKDTWLRPRLREVQKLLEGYLG
                     VCQEMGAGPRVSALSEAILALHGRAGAAIESLSSGYGHAARWQQ"
BASE COUNT          719 a         1743 c         1615 g          780 t
ORIGIN      
        1 tttcgaggcc cgggccaggc gcgtcaagct gtccagcagg atcgtcacgt ctcgcccatg
       61 ctcaaccagc cgcttggccc gctcgaggca gaagtcggcc gcccgcatat ggttctcggg
      121 tagctggtcg aaggtcgagg cgatcacctc acctcccgac cccaccgcgc gtgagatgtc
      181 ggtcacctcc tccgggcgct cgtcgaccaa caggaccagc agcaccacct cgggattgtt
      241 ggtgatcagg gcgtgcgcga tgtccttcag gatggtcgtc ttccccgcct tgggtggtgc
      301 gacgatcagg ccgcgctgcc cctttccaat gggggagatg atgtcgatca gccggttcga
      361 cagccggttc cgatcggttt ccagcacgaa acgctccgtt gggaagatcg gcgtcagatc
      421 ctcgaaatcg gggcggttcc ggcacgactc aggctccgcc gagttcaccg actcgatccg
      481 gagcaacgag taatagcgct cactcgtgcc gtcacggggc ggccgcgctt ctccgacgac
      541 cgtgtcaccc gtacggagtc cgaatcggcg gatctgggac tcggacacgt acacatcatc
      601 gttggcaggc aggtaacccg cgcggcggag aaatcctcgg ccctcgggca gtatctcgag
      661 gatgcccgtg accagatgcc gagcgtcgcc ctctgcctcc ccccggccat cccggggctc
      721 ccgcatgtcg cggctgtccc cccggccgcg ccggtccggc cgcgcatcct gctgaggccg
      781 gccatcgcct cgaccgtcgt ggcgctggcc actgcgctgg tccgggcgac cgtccactcg
      841 aggcgcctcg tgccgcgagt cgccgttccc accacggtcg cctcggcgcg aacgtccgct
      901 caggcgacct cgcgtgtact cgtaccgcgg ctcgtgccgg tcgcgatcca cggtctccgt
      961 gtgccgcggg ggttcctcct cggccgcgtc cggccgatcg ggcttatcgc gcatcgtaac
     1021 accggggagt tcgacctccg cccccatggg cggaaccgga accgactcgg ccggatcggg
     1081 tgtcccgaac gtcacagggg ccgacggaac cacgaagccc gtcggagccg gcggcgcggc
     1141 cgtcggcatg ggcgccgtcg aagcgatgga gccctgcggt tcatgctgct gctccggctc
     1201 gggctccggc tcgaggtccc tgctcgcctc gacatcccgc tcttccgcat cgtggcgaac
     1261 cccggtgggg gccccttggc gcccgctgcg accgcggcgt cgcggcgccg ttgactctgc
     1321 gggggccgcc gcctccggcc gcgctgcctc gggggctgac accggcatct cggaccgggc
     1381 aggctccgca ggtgccacgg gcccgggcgc gggcgcatcc acaccggcct cgagcaggcg
     1441 agccaccagg tccgccttct tcagtcccgt gatgccctcg agcccccgcg ccttggccag
     1501 cgctcggagc tggggaagag tcttcgagtg gagatcagaa gcggacatgg acaggcctcc
     1561 ggtgctcggc acgcgatcct gccattacgg acatagcgcg cgagcgagga cgtagataag
     1621 ggatcggggc ggcccaacgg gccgtagcgc ccacagcctc ctgaggagga caactaccag
     1681 aaggactcgc aaccaagcac ggggggcgtc tcaccggact gggcccctcc ggacccaaac
     1741 cccaaacggc gtaaccagaa ctccctgtgg gacacccaac ggcatgtacc ctgaaatgac
     1801 ctctcggcac cagtgggtct tgctccagtc cccgagtcgc ggctctcgct ccggcggtgc
     1861 ccgggatctg aggcgctggt ccactcccgc actcgcgtca cccggtccgg tcgccggtcc
     1921 cggaccctgc cccgccgcga ggcttcgaac atagcctttg cgcccggcca cgaggaacgg
     1981 gatgggcggc gggccagtgg gttgtgaggt tatcgagtgg ggagacgctg tgaagcgcct
     2041 gcattttact cgacggaccc atacgcgtca acggctcgat cggccgccgc gttcaccggc
     2101 cgcgccgctc cggtcgttga cccgccacct gtgggtccgg ccagtgccgt ctcgggctcc
     2161 gctggcaccg gcggcgcctt gggcctgcca acaaggcaca catgcagcga gtcaccctcg
     2221 acccaactcc gcaccagcag cgcggcgtcg gtggcgttgc ggaacttcat gtcggccgtc
     2281 ccccacgaga ccgtggcgtc ccgcccccag ggcacatagg gcacgctcct cgagtgggga
     2341 tggcgttcga cgatgggcat gcctgccatc agggccgcgt tgtagaccgt cgttgagacc
     2401 tggcagacgc cgccaccgta tcccaggaca acccggctgc ccgagtagac attggccttc
     2461 agatacccac tctgcgggct gcgctgcccg gtgctcgcgt tgagcgagaa aacgccacct
     2521 gccgggatcc aggtgtcgtc caccgagctc accgccagac ggacgttctg tgtgcggttg
     2581 gcctgcgaag cgttgaaccg ggttgcacag tgccccagca cccggtaccc ggccagcgcc
     2641 tggcgcgtcc gcccgggcgg tgtgaatacc aggggcagct cgatcacccg gcggacggcc
     2701 ccttcggtca gggctttctc gacggccgtc actgctcggt ccacatccag ggcaacgccc
     2761 acgacttcag ggacgatcac cgcccggatt ccggcgatac gaatggtggc gtcccgcggc
     2821 gcgcgccgca cggcctcgcc cacggcaacc agccgctgac ggagcttggt ccgatccatg
     2881 cgcacactca gcggcacgtc acggggcccc gagtcgcccg gatcccgcga cgcctccttc
     2941 agtgcaccgt cgacgtcgaa ggaggccccc aaggacgcga gcgccgtcac ccgagcggta
     3001 ctgccgcaca ccagcgttac ggggcctcgc acaagctgct ctgcgcgtgc ggcgatggcg
     3061 gcgcgagcgt cctcgagcgt tctgcctcca acggccacgc cggcaacgga agtcccgtca
     3121 ggcaacaggg ctgccgtgtc ggcccggttc agcccgaaac ccatcaggaa cagaggcgct
     3181 agaagcagag gtatggtacc cggactcgcc catggtacgg cccgagccag ccactccacc
     3241 ggagacggtc cctcccgccg tgccgagtcg aaccttcggg cgcgccccca accgtgtgga
     3301 tcgcgcgggt tcctataata cggtggtctg cgttccctcg ccaaccgcca ctcgttgcgg
     3361 aatcgtgtgg tgcaggaagg gagatggcgt gggaacggga actgtgcaag cactcgactt
     3421 gtccagcgtg ggaacgcgcg ggctgctggt cgccccgccc acgggggcac gaacgtcgcg
     3481 gtgcgcttgg ggtcgcctgg tcaactggat atggggggcg cctgactcgt actccggggt
     3541 gagagcaatg gtgcgtagca gtgtaacgac ggtagcccgg ctcgcgtggc ttgtcccgag
     3601 cctggttgcc ttctcggccg ggcttggctg gtgccaagag gcggtggctc ctccagcggg
     3661 tggggcacct gcgccggaca cggcgcctct gggcacccct cgggcccccg tggttccggc
     3721 cgcgggaacg ccggcccagc aggcgagcct caccaccccg ctcatcagtg tcgcccgcac
     3781 tggcgtcgcg cccgacatcg acggactccg caccgagccc ctgtggtcgc gcgccatgcg
     3841 tatctcgcac ttcgtgcgcc tcgacggcgc cgcgcggccc gagaaccaga ctgaggtctt
     3901 cttgacttat gacgatagcg cgctctatgt ggccatctcc tgcgaagagc cccaactcga
     3961 gacgctttcg gaccgggcca aggtgcgaga ctccgaggag atcctccgtg acgacttcgt
     4021 cgagctgctg attgccccga gccgtaccag tcccgcctac tcccgaatcc tggtcaaccc
     4081 cgccggtacc gtcgccgatc tgggcggggc cgacggctcg gtcacctgga catgcgaggg
     4141 gcttcgggtc ggcgcccaga gaggtagcct ccggtggctc gtcgagctcg ccattccctt
     4201 ctccgcgctg ggtgccaccc cgacggacgg ggacgtgtgg ggcttcaacg tggcccgcta
     4261 tcgcgcgcgg gacggtgagt tctcctgcta tgccggcgcg gggactggcg tgggctaccg
     4321 gagtcccggt aacttcgcca ctctggtttt tgccggatcc accatcatcg tcgacctggt
     4381 ggacattggc gacttgttcg gtgagctcaa gggcggcgcc gaactcaagg tgaggcttcg
     4441 ctcgactcag ccccgtcggg cgacgcccgt ggcccgcgtg atccgcggat tcgatgagcg
     4501 gcgcttcggc tactccggtg tcaccgtcga cgtgcccgcc ctcaatccgg cgcgaatcgt
     4561 gatcccctac gaggtcgatg attcccgggg ggccgtgatt cagctgggcg tcctcgatga
     4621 cgccggcgcc accgtgttcc tcagccccaa gatcccactc aaggacacct ggctccggcc
     4681 gcgcctgcgt gaggtgcaga agctgctcga ggggtacctc ggcgtgtgcc aggagatggg
     4741 tgctggcccc cgggtcagtg ccctctcgga ggccatactg gccctccacg gcagggcggg
     4801 ggcagccatc gaatcgctct ccagcggcta cgggcacgcg gcccgctggc agcagat
//
LOCUS       DAOSPL010000118         4831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_17645_length_4831_cov_2.964405, whole genome shotgun sequence.
ACCESSION   DAOSPL010000118 DAOSPL010000000
VERSION     DAOSPL010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17645_length_4831_cov_2.964405"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..1939
                     /locus_tag="PLQ54_03345"
     CDS             197..1939
                     /locus_tag="PLQ54_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOS92322.1"
                     /translation="MGPVTIRGNHFIQDGRPLQIISGAIHYFRVVPDYWSDRLLKLRA
                     CGFNTLETYVAWNRHEPRPGVFDFSGMLDIERYLHLAHEHGLMAIVRPGPYICSEWDL
                     GGLPSWLLADGNMQLRCAYRPYLDAVDRFLDALMPRLTPLQASRGGPIIAMQVENEYG
                     SYGSDKAYLSHLERGLRDRGFDGLLFTSDGATDWMLEGGTLPHILKVANFGSDPAGNF
                     AKLREHQPEGPMMCGEFWAGWFDHWGEKHHTRDPEDAAKCLDEMLASGASASIYMFHG
                     GTNFGFLNGANHSDRYEPTITSYDSDAALNEAGDPTPKYHAFRRVVSRYTPLPDIDVP
                     CPSAKAAYGRVELTGQAKLFDALDALSSPLERPTPDPMELVGQDYGFILYRTTVRGPR
                     EPLPVVLHEVHDRAQVFVDGEFRGVIERSGPQEDLVLGFEPGEHALDVLVENMGRINY
                     GPLLHDRKGITRGVRLGNQFLYGWTIWPLPLDDLTGLSFRPRADLSGPAFLRGTFRVD
                     DARDTFLATPGWSKGACFLNGFNLGRYWERGPQRTLYVPAPLLRPGENEIIVFELHGA
                     TEPFVEFRDVPDLG"
     gene            1976..4477
                     /locus_tag="PLQ54_03350"
     CDS             1976..4477
                     /locus_tag="PLQ54_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016161 - beta-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 14 glycosylhydrolase"
                     /protein_id="HOS92323.1"
                     /translation="MPLAMLLAWVPVSLVQQVAPEDVFVEAESAASHTFSNVADFPGI
                     VSGGRILRLWEGNDPPADGYWADLPFAVAGQGDYHVWLAASLPGSTSPFWWRVDDGPW
                     GHVTDDMLLEVGETFGVSGVMAWIELTTQRLAAGGHRLRVRVDERRPGLEKAHLLYLD
                     AMLVTPRDIMPRGLVTPADLPNLAPRPAPPVPVHRAGTPGEPMMLGTSVMGHSQNLLV
                     KSIGFSLSQTDSDHLTVNETAPGVWDWAAADAGLEACRKAGLRWQYFPHWHWPPEWYR
                     ATDRFVPCTGLRSGRKLACMSLWSPDILPWFDHCYTAMAEHYGSGADPVAAIYVGVHG
                     DFGETIFPMGFHPVERERFGPEGTGVADFWCAAEPDRAAFRVWASERYESLEALNQAW
                     GTDFAGWDVLDYPPAAYSNEADVKSTPEVRRRWLDFIDWYFGSMTQFTADVCRIARRH
                     FPEAMLVLPVGGGAESLVYGQDNTALPKIAAELGVHIRSTHGGFQPFADNYSTINRRI
                     ATASKFYGAPFWTEPPGSISATLEVSRIAESIACASWGFWDWGSNPVAAADVFREYAA
                     FFTREKPVVDVALLFPTTNYRLNWLNAHSPKLAKMGAELRDVMDYDIIDEMLIADGAL
                     DRYRVLAWLEGDWVEASTLRKLADWIEAGGVLFRVGAEPMQDVSGDAALAREFLGVTD
                     AAPGPGGGSGPLVVADFARHLKGTPEPVVAATASPLAEGAQMLAQAGGAPAAWAVPRG
                     RGWVIVWAGMCNDDRERRTASELLRDVVYNLSALDGTKADALELDTAWDSVYTTLLAN
                     GEAVVYNDSTEPRAVTIGGQTLDLPPKSLRSALAK"
     gene            4623..>4831
                     /locus_tag="PLQ54_03355"
     CDS             4623..>4831
                     /locus_tag="PLQ54_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92324.1"
                     /translation="MWTTEQLLAEAVGGRVGGRFVGMDEIDGMEADALKRAAEQALAR
                     PQASEFDPGARPLSRRLLAAQWTLVL"
BASE COUNT          717 a         1694 c         1634 g          786 t
ORIGIN      
        1 tactcggcgc cggctctcac atcggtcgac ctgccggttg ccgagatggc cgagggagct
       61 gtgcggctcc tcctgtccct ggttggctgc gacggcacgg tgccgacgcg catcatcgtc
      121 aggcctcgcc tggtggtgcg cgagtccacc gcgccgccac gaccgcactg acggaccatc
      181 tcttcgagga ggacccatgg ggccggtcac gatccgcggc aaccatttca tccaggatgg
      241 caggcctctt cagatcatct ccggcgccat ccactacttc cgtgtggtgc ccgattactg
      301 gtccgaccgg ctgttgaagc tccgcgcctg cgggttcaac acgctggaga cctacgtggc
      361 ctggaatcgc cacgagcccc gccccggtgt gttcgacttc agtggcatgc tcgacatcga
      421 acggtacctg cacctggccc acgaacacgg cctgatggcc atcgtccggc ccgggcccta
      481 catctgctcg gagtgggacc tgggcggcct gccctcgtgg ctgctggccg atgggaacat
      541 gcagctgcgc tgcgcgtacc ggccgtacct ggatgccgta gatcgcttcc tcgacgcact
      601 catgccgcgc ctgacgcccc ttcaggcatc gcggggtggg cccatcatcg ccatgcaggt
      661 cgagaacgag tacggcagct atggtagtga caaggcgtac ctctcacacc tggagcgcgg
      721 cctgagggat cgcgggttcg acggcctgct gttcacctcc gacggcgcca ccgactggat
      781 gctcgagggc gggacgctcc cgcacattct caaggtcgcc aacttcggct ctgacccagc
      841 ggggaacttc gcgaagctcc gcgagcatca gcccgagggc ccgatgatgt gcggcgagtt
      901 ctgggccggc tggttcgatc actggggcga gaaacaccac acgcgcgacc cggaggacgc
      961 cgccaagtgc ctcgacgaga tgctcgcctc aggcgcgtcg gcgagcatct acatgttcca
     1021 cggaggcacg aacttcggct tcctcaatgg cgccaaccac agcgatcgct acgagccgac
     1081 catcaccagc tacgactctg atgccgcgct gaatgaggcc ggcgacccga cacccaagta
     1141 ccatgccttc cggcgcgtcg tgtcccgcta cacgccgctg cccgacatcg acgtcccgtg
     1201 cccctcggcc aaggccgcgt acggccgtgt cgagctcacc gggcaggcca agctgttcga
     1261 cgcactcgat gccctgtcgt cgccgttgga gcgtccgacg cccgacccca tggaactcgt
     1321 gggtcaggac tacggcttca tcctctaccg aaccacggta cgcgggccgc gcgagccgct
     1381 gcctgtcgtg ctccacgagg tacacgaccg ggcccaggtg ttcgtcgacg gtgagttccg
     1441 tggcgtcatt gagcgcagcg ggccgcagga ggatctggtg ctcgggttcg agccgggcga
     1501 gcatgcgctg gacgtcctcg tcgagaacat gggccgcatc aactacggac ccctcctgca
     1561 cgaccgcaag ggcatcaccc gcggcgtgcg gctcgggaac cagttcctgt acggttggac
     1621 gatctggccc ctgccactcg acgacctgac ggggctctcg ttccggccgc gggccgatct
     1681 gtcgggtccg gccttcctcc gcggcacgtt ccgcgtcgac gacgcacgtg acactttcct
     1741 ggccacgccc ggctggagca agggcgcgtg cttcctcaac ggcttcaacc tgggacgcta
     1801 ctgggagagg gggccgcagc gcacgctcta tgtgccggct ccactcttgc gcccgggcga
     1861 gaacgagatc atcgtgttcg agctgcatgg ggctacggag cccttcgtcg agttccgcga
     1921 cgtccccgac ctgggctagg ggggcgccgc gtcgttctgt gtccgggagg tcgccatgcc
     1981 cctcgcgatg cttctcgcat gggtgccggt ctcgcttgtc cagcaggttg cgccggaaga
     2041 cgtcttcgtg gaggccgagt ccgcggccag ccacacattc agcaacgtgg cggacttccc
     2101 cggcatcgtg agcggcggcc gcatcctccg cttgtgggag ggcaacgacc cgccggccga
     2161 cgggtactgg gcggacttgc cgttcgccgt ggcgggccag ggggactacc acgtctggct
     2221 ggcggcttcg cttccgggat ccacgagccc cttctggtgg cgtgtcgacg acggcccctg
     2281 gggccatgtc accgacgaca tgcttctcga ggtcggcgag accttcggcg tgtctggcgt
     2341 gatggcgtgg atcgagctca ccacacagcg cctggctgcg ggcggccaca ggctccgggt
     2401 gcgggtggac gagcgccgcc ccggactcga gaaggcccat ctgctctacc tcgacgcgat
     2461 gctcgtcacg ccgcgggata tcatgcctcg cggccttgtg acgccggcgg acttgccgaa
     2521 cctggcgccc cggcccgctc cgccggtgcc cgttcaccga gcgggcacac caggcgagcc
     2581 gatgatgctc ggcacatcgg tgatgggcca cagccagaac ctgctcgtga agtccatcgg
     2641 gttctcgctg tcgcagaccg actcggatca cctgacggtc aacgagaccg cgcccggtgt
     2701 gtgggactgg gctgcggccg atgccgggct cgaggcctgt cgcaaggccg ggcttcgctg
     2761 gcagtacttc ccccactggc actggccgcc cgagtggtac cgcgccaccg atcggttcgt
     2821 gccgtgcacg ggcttgcgca gcggccggaa gctggcctgc atgtccctct ggagcccgga
     2881 tatcctaccg tggttcgacc actgctacac ggcgatggcc gagcactacg ggtctggggc
     2941 tgatccggtc gccgccatct acgtcggcgt ccacggcgac ttcggcgaga ccatcttccc
     3001 catgggcttc cacccggtcg agcgggaacg cttcggcccc gagggcacgg gcgtggcgga
     3061 cttctggtgt gccgctgagc cggaccgggc cgccttccgt gtctgggcga gcgaacggta
     3121 cgagtcgctc gaggccctga accaggcttg gggcaccgac ttcgccggtt gggatgtcct
     3181 ggactatccg cccgccgcct acagcaacga ggccgacgtg aagtccacac cggaggtgcg
     3241 ccggcgctgg ctcgacttca tcgactggta tttcggcagc atgacacagt tcacggccga
     3301 cgtatgccgc atcgcccgac gccacttccc cgaggccatg ctggttctgc cggtgggcgg
     3361 cggcgccgag tccttggtgt atgggcagga caacacggcg ctacccaaga tcgccgccga
     3421 actcggcgtc cacatccggt cgacccacgg cggcttccag cccttcgctg acaactacag
     3481 tacgatcaac cggcgcatcg ccacggcctc caagttctac ggcgcgccct tctggaccga
     3541 gcccccgggc tcgatctcgg ccacgctcga ggtcagccgg atcgccgagt ccatcgcctg
     3601 tgccagttgg ggcttctggg actggggcag caacccggtg gcggccgccg acgtgttccg
     3661 cgagtatgcc gccttcttca ctcgggagaa gcccgtcgtc gacgtagcgc tcctcttccc
     3721 caccaccaac taccggctca actggctcaa cgcccactcg ccgaagctcg cgaagatggg
     3781 cgccgagctg cgcgacgtga tggactatga catcatcgac gagatgctca tcgccgacgg
     3841 cgccctcgac cgctaccggg tgctggcatg gctcgaaggc gactgggtcg aggcatcgac
     3901 gctgcggaag ctggccgact ggattgaggc aggcggcgtc ctgttccggg tcggtgccga
     3961 gccgatgcag gacgtctccg gcgatgcggc gctcgcccgc gagttcctcg gtgtcaccga
     4021 cgccgcaccc gggccgggcg gggggtccgg gccgctcgtc gtcgccgact tcgcccggca
     4081 cctgaagggc acgcccgagc cggtggtcgc ggccacggcg agcccgctgg ccgagggagc
     4141 gcagatgctg gcgcaggccg gcggcgcacc cgcggcctgg gctgtgccgc gcgggcgcgg
     4201 ctgggtgatc gtctgggccg gtatgtgcaa cgatgaccgc gaacgccgta cggcctccga
     4261 gctcttgcgg gacgtggtct acaacctgtc ggcgctcgac gggaccaagg cagacgccct
     4321 ggagctcgat acggcctggg atagcgtcta caccacgctc ctggccaatg gcgaggcggt
     4381 ggtctacaac gactcgacgg agccccgcgc ggtgaccatc ggcggccaga ccctcgacct
     4441 tccgccgaag tcgctgcgga gcgcgctggc gaagtagcgg cagcggccgg ggaccccggt
     4501 cccctggtct tgcggagggg ccagataggg ggaaccgtgg gccggtaggc cgatggtggg
     4561 accctaatcg gcccgggtcg gccgtcaggc cgacggatcg acccaaggag gcaaccaaca
     4621 ccatgtggac caccgagcag ctcctggccg aagccgtcgg cgggcgcgtc ggcgggcgct
     4681 tcgtcggcat ggacgaaatc gacggcatgg aggccgatgc gctgaagcgc gccgccgagc
     4741 aggccctggc gcggccccaa gcctccgagt tcgatcccgg cgcccggccg ctcagccggc
     4801 ggctcttggc ggcccagtgg acgctggtgc t
//
LOCUS       DAOSPL010000119         4826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_17660_length_4826_cov_3.260532, whole genome shotgun sequence.
ACCESSION   DAOSPL010000119 DAOSPL010000000
VERSION     DAOSPL010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17660_length_4826_cov_3.260532"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PLQ54_03360"
     CDS             <1..978
                     /locus_tag="PLQ54_03360"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOS92325.1"
                     /translation="ALDQVPGPAEPVESLGLALDRAVSRVRRQGELEAERGRLLALIA
                     KARAAAERRREKLAAARVPEHELDRLRHAGESILAALPIIPPGARQVQVPDLYSPTGE
                     SMTVELDPAKTPQANAEVYFRRYRSARSQAEKLPALVAQVEAELVELGDLEARVRGAD
                     ERRISGAAAEVEGHRLLQPYAQRGRSGRPSSGPRFLSCSSRDGFELLVGRGASESDEL
                     LRTVAAPDDIWFHARDIHGGHVLIRTARHPERVPMATILEAAAIAAHYSKAKHSALVP
                     VDYTLRKHVRRARKGSAGLHIYERAKTVMVEPLSPAQMRQDATDMERRS"
     gene            975..1637
                     /gene="rdgB"
                     /locus_tag="PLQ54_03365"
     CDS             975..1637
                     /gene="rdgB"
                     /locus_tag="PLQ54_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOS92326.1"
                     /translation="MTIVVATRNPGKLVEIRSALERAAPGLALRSLCDYPDLAELPET
                     ADTFEGNAILKATALAGQVGLPALADDSGLSVDALGGFPGVHSARFAGPAATDADRNA
                     ALVARLRESGIPPDRWMARFVCVLAVASPRGLCATYRGECQGRIIEEPRGSNGFGYDP
                     IFLRPGDGRTMAEHTRDEKNACSHRGAALAALVADLPALLRRLGAAATAGEATQRVRG
                     IR"
     gene            1634..2206
                     /locus_tag="PLQ54_03370"
     CDS             1634..2206
                     /locus_tag="PLQ54_03370"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOS92327.1"
                     /translation="MSKKILEPSTRLFPIPAALVSCVGSDGVPNIITIAWTGVVCSEP
                     PMISVSIRPKARHSYPIVKSSNEFVLNIPSASQVRTVDWCGTVSGSRVRKFDEAGLTP
                     AVASVVRAPLIAECPVNIECEVRHRLALGTHDCFIGEVVAVHADDAVLDADGRISVGA
                     VEPLAFCDGDYWSIGSRVGVYGFSRGKLTD"
     gene            2272..3294
                     /locus_tag="PLQ54_03375"
     CDS             2272..3294
                     /locus_tag="PLQ54_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS92328.1"
                     /translation="MDVIRTQNLTKIYGHRKIALNAVELRVPKGSIFGFLGPNGAGKT
                     TAFRLMLGLQRPTAGRVEILGKRVGPNSSDVRRHVGYLPTNPEFPPDMTPITYLDFVG
                     VLCGLSREARSPRLGSLVRAVGLLGAQSQRIGTFSTGMKTRLGIAASLMNDPSILLWD
                     EPCSGLDPAGRRHTMDLITWLGRTKTIVVSSHILGDIDQVCTHVGVLSDGQLIFSGTM
                     GEMKQRIHVDTLVVELSGGDGVLERFRAASRAIEGLDGLEREGSRFHLRPVPGASLGE
                     LVAAVAGSVREAGGDILSLSTGQGRMEDAYLQMLEQDGARGFLRACGDAEGGEANGCV
                     RGTPAS"
     gene            3263..3979
                     /locus_tag="PLQ54_03380"
     CDS             3263..3979
                     /locus_tag="PLQ54_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92329.1"
                     /translation="MGAYVALLHRDLVTIARSWVARGWLVLSALAAAVAVLGQSTQGL
                     PASQVAAQIQLTFLFLWSIFVIVIGASAVAPESGVLADSVLSRPVTRSQYILAKLTAR
                     SFAVLVVFVSVAGGSAYATWRYSKLPDVSFADVSLGVVHVLTALLFLVAVSVAMSSVM
                     RTTFVAIVALVLAWYSVGALFEFLNVSFLSPFELRTSLPECLRGELDADLLWKIPLGF
                     GIPTVAVWLATLAYFNYKDL"
     gene            4063..>4826
                     /gene="folE2"
                     /locus_tag="PLQ54_03385"
     CDS             4063..>4826
                     /gene="folE2"
                     /locus_tag="PLQ54_03385"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HOS92330.1"
                     /translation="MASERDPRGIAIQRVGIRGLSLPTLVSVKSGGHTRVSATIDMSV
                     DLPREFRGTHMSRFVALLGEWQDRPFGRQEIEKLLTTAKRRFGSTSAFISVRFKYYVA
                     KQAPVSRTPFHMDYNCLFEGELNGNSLVFHLGVEVPVLTLCPCSKEIAEAGAHSQRAA
                     VRVLLQNDYGRIVWIEDVVAMLEAQGSTPVYPFLKRADEKLLTENAYANPKFVEDVVR
                     DAVIELSKLDHVPWFRVECESFESIHNHNAYAMYETP"
BASE COUNT          716 a         1525 c         1691 g          894 t
ORIGIN      
        1 gccctggacc aggtgccggg cccggccgag cccgtcgaga gtcttgggct ggcgctcgat
       61 cgagcggtga gccgtgtgcg gcgccagggg gagctcgagg cggagcgggg tcggctcctc
      121 gccctgatcg ccaaagcgag ggcggcggcg gagcggcgac gcgagaagct ggcggccgcg
      181 cgggtgccgg aacacgagct cgaccggttg cgccacgccg gtgagtcgat cctggccgct
      241 ctcccgatca ttcctcccgg cgcccgccag gtgcaggtgc cggatctcta cagccccacc
      301 ggtgagagca tgacggtcga gctcgatccg gcgaagaccc ctcaggccaa cgcggaagtc
      361 tacttccgcc ggtaccgcag cgcccggtcg caagcggaga agttgcccgc ccttgtggct
      421 caggtcgagg ccgagcttgt cgagctcggg gacctcgagg cgcgagtcag gggtgccgac
      481 gaaaggcgca tctccggggc ggccgccgaa gtcgaaggcc atcggttgct ccagccctac
      541 gcccagcgcg gccgttccgg ccggccgtcg agcggcccga ggttcctgtc gtgttcgtcg
      601 cgcgatggct tcgagctgct ggtcggccgc ggcgccagtg agagcgacga gctcctgcgc
      661 accgtggccg cgccggacga catctggttc catgcccgcg acatccatgg cgggcatgtg
      721 ctgatccgaa cggctcggca tcccgagcgg gttcccatgg caacgatcct ggaagcggca
      781 gcgattgccg ctcactactc gaaggcgaag cactcggcgc tggttccagt ggactacaca
      841 cttcgcaagc atgtgcggcg cgcccgcaag gggtcggccg ggctccacat ctacgagcgc
      901 gccaagacgg tgatggtcga gccgctctcg ccggctcaaa tgcgccaaga cgccacggat
      961 atggagcgac gttcgtgacg attgtcgtcg ccacccgcaa cccggggaag ctggttgaga
     1021 tccgctcggc gctcgagcgc gcggcccccg gcctggcact gcggagcctg tgtgactacc
     1081 ccgacctggc ggagttgccc gagacggcgg acaccttcga gggcaacgcg atactgaagg
     1141 cgaccgcgct ggccgggcag gttgggctcc ccgctttggc cgacgactcc ggtctgtccg
     1201 ttgatgcact gggtggcttc ccgggagtgc actcggcacg tttcgcgggt ccggcggcga
     1261 cggacgcgga ccgcaacgcg gccctggtgg cgcggcttcg ggagagcggc atccctccag
     1321 accgctggat ggctcggttc gtgtgtgtgc tcgcggtcgc gtcgccacgg gggctctgcg
     1381 ccacctaccg gggtgagtgc cagggccgga tcatcgagga acctcggggc agcaatggct
     1441 tcgggtatga cccgatcttc ctccggccag gtgacggccg gacgatggcg gagcatactc
     1501 gggacgagaa gaacgcctgc agccatcgtg gcgcggccct ggccgcactg gtggccgatc
     1561 tgccggcact tctgcggcgc ctcggcgcgg ccgccacggc tggagaggcg acacagagag
     1621 tgagaggaat tcgatgagta agaagatttt ggagccttcg acgcggctgt tccccatccc
     1681 ggcggcactg gtctcgtgtg tcggcagtga cggcgtgccg aacattatca ccatcgcttg
     1741 gaccggcgtg gtatgttcgg aaccgccgat gatcagtgtc tcgatccggc ccaaggcccg
     1801 ccactcgtac cccattgtga agtcgagcaa cgagttcgtc ctgaatatcc cgtcggcgtc
     1861 gcaggtgcgg acggtggact ggtgtggcac ggtctcgggc tcgcgggtgc gcaagttcga
     1921 tgaggcgggc ctcacaccgg ccgtggcctc ggtggtccgc gcgccgctca tcgccgagtg
     1981 tcccgtcaac atcgagtgcg aggtccgtca tcgtctggcg ctcggcacac acgactgctt
     2041 catcggcgag gtcgttgccg tgcacgcgga cgatgccgtc ctcgatgccg atgggcgcat
     2101 ctcggtgggt gcggtggagc ccctggcctt ctgcgacggc gattactggt cgatcggctc
     2161 gcgcgtcggc gtctacggct tcagccgggg caagctgacg gactgaccgc tactctgctg
     2221 aaacccgcgc cgcagaagcc cgtcctcgcg tggggggagg cggtggcacg gttggatgtt
     2281 atccgcacgc agaacctgac caagatctac gggcaccgga agatcgccct caatgccgtg
     2341 gagctgcggg ttccgaaggg ttcgatcttt gggtttctcg ggcccaacgg tgccggcaag
     2401 acgacggcct tccggctgat gctgggtttg cagcgcccta cggccggccg tgtggagatc
     2461 ctgggcaaac gggtcggccc gaactcgtcg gatgtacggc gccatgtggg ctacctgccc
     2521 acgaaccccg agttcccgcc cgatatgacg cccatcactt acctcgactt cgtcggggtc
     2581 ctgtgcggtc tttccaggga ggcccgaagc cctcggcttg gctccctggt ccgtgcggtg
     2641 gggctcctgg gcgcgcaatc gcagcgcatc gggaccttct cgaccggaat gaagacccgc
     2701 ctgggcatcg cggcgtcgct gatgaatgat ccctcgatcc tgctgtggga cgaaccgtgt
     2761 tcggggctcg atccggcggg tcgtcggcac accatggacc tgattacctg gctgggtcga
     2821 acgaagacca tcgtcgtttc ctcgcacatc ctgggtgata ttgaccaggt gtgcacccac
     2881 gtgggcgtac tgagcgatgg tcagctcatc ttctcgggga ccatgggcga gatgaagcag
     2941 cgcatccacg tggacacgtt ggtggtggag ctctcgggcg gcgatggtgt cctcgagcgg
     3001 ttccgcgccg cttcgagggc catcgaggga ctggatgggc tcgagcgcga gggttcccgg
     3061 tttcacttgc gcccggtgcc gggagcctcg ctgggcgagc tggtggcggc cgtggccggg
     3121 agcgttcgag aggcgggggg cgacatcctg tcgttgtcca cgggacaggg gcgtatggag
     3181 gacgcctatc tgcagatgct cgagcaggat ggcgcgcgag ggttcctgag ggcctgtggg
     3241 gacgcggagg ggggagaggc caatgggtgc gtacgtggca ctcctgcatc gtgacctggt
     3301 gaccatcgcc cggtcgtggg tggcgcgcgg gtggctggtg ctgagcgccc tggccgcggc
     3361 cgtcgcggtg ctcgggcaat ccacccaggg ccttcccgcc tcgcaggtcg cggctcagat
     3421 ccagctgacc ttcctgtttc tgtggagcat cttcgtcatt gtgatcggcg ccagtgccgt
     3481 ggcccccgag agtggcgtcc tggccgattc cgtgctgagc cgtcctgtga ctcggtctca
     3541 gtacattctg gcgaagctga cggcccggtc gttcgccgtg ctggtggtct tcgtcagcgt
     3601 cgcgggcgga tcggcctatg cgacctggcg ctactcgaag ctgcccgacg tctccttcgc
     3661 cgatgtctcg cttggcgtgg tgcatgtcct cacggccttg ctgttcctgg ttgcggtgag
     3721 tgtcgcgatg tcttcggtca tgcgaacgac ctttgtggcc atcgtggccc tggttctggc
     3781 ctggtactcc gtgggcgcgc tgtttgagtt cctcaacgtg tccttcctct ctccctttga
     3841 gcttcggacg agcctacctg agtgtctccg cggcgaactg gacgccgatc tgttgtggaa
     3901 gatccccctc ggcttcggca tccccacggt cgccgtgtgg cttgccaccc tcgcgtattt
     3961 caactacaag gacctctagg cggcggagta ttgcccgctg gaggcagccg ggccacgcgc
     4021 gtggcggccg gtgagcggcg agggtgacgg cagttgcgcg atgtggcgag cgaacgcgat
     4081 ccgagaggga tcgccatcca gcgtgtgggt atccggggcc tctccctccc gaccctggtg
     4141 tccgtcaagt ccggcggtca cacgcgggtc agcgcgacga tcgacatgag tgtcgatctg
     4201 ccgcgggagt tccgcggaac tcatatgagc cggttcgtgg cgctgctggg cgaatggcag
     4261 gaccgcccgt ttggccggca ggagatcgag aagctcctga ccacggccaa gcgccgattc
     4321 ggttcgacca gtgcgttcat ctcggtgcgg ttcaaatact atgttgcgaa acaggctccg
     4381 gtgagccgta cccctttcca catggactac aactgcctat tcgagggcga gctgaatggt
     4441 aattcgctcg tctttcacct gggtgtcgaa gtgcctgtcc tcacactctg cccctgctcg
     4501 aaggagatcg ccgaggccgg cgcgcacagc cagcgggccg ccgtgagggt tctgctgcag
     4561 aacgactacg ggcgcatcgt atggatcgaa gacgttgtgg ccatgctgga agcccagggc
     4621 agcacgcccg tgtacccctt cctcaagcgg gccgacgaga agctactcac cgagaatgct
     4681 tatgccaacc ccaagttcgt cgaggacgtg gttcgggacg ccgttattga gctctcgaag
     4741 ctggaccatg tgccgtggtt tcgtgtcgag tgtgagagct tcgagtcgat acacaatcac
     4801 aatgcgtacg cgatgtacga gacgcc
//
LOCUS       DAOSPL010000120         4821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_17685_length_4821_cov_3.323542, whole genome shotgun sequence.
ACCESSION   DAOSPL010000120 DAOSPL010000000
VERSION     DAOSPL010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17685_length_4821_cov_3.323542"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..919
                     /locus_tag="PLQ54_03390"
     CDS             134..919
                     /locus_tag="PLQ54_03390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS92331.1"
                     /translation="MVVVVTTARRPTPELRSGAEELATELGLEVVERAGESISALRQR
                     HGADGVLVVAHDGITYRVGDVTFFYHPSMAKVRVRGLQLGARDVTVDAMGLQPGWRVL
                     DCTLGRASDAAVAAHVVGTSGRVVGLEIDPIIAALTRRGLLTFDPDSRALSEALRRID
                     VICADHADYLAQASSRSFDVVYFDPFFEETVEASGAMQDLRAVGSHGAVTADSLAEAA
                     RVARRRVVFKTRRGLCPPLVAAWRAVAGGKSRVEYRVLDIEES"
     gene            922..1410
                     /locus_tag="PLQ54_03395"
     CDS             922..1410
                     /locus_tag="PLQ54_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOS92332.1"
                     /translation="MYRPGGRTVIVLLGIAVVVLAGRWAPPPPRGEIAAPRQTVWVER
                     AVDGDTLQLRGLGRVRVIGVDTPEVGEPGYAEATEFTRKTCARRLVEIEVCPVRPVDR
                     YDRVRAMVYYRDDAGREHSLADELIRRRLGHALSIQPCHIPDSHWYDLQSQRPPGEPD
                     GE"
     gene            1416..1784
                     /locus_tag="PLQ54_03400"
     CDS             1416..1784
                     /locus_tag="PLQ54_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92333.1"
                     /translation="MRCRECGASLPEDAVECAECGGRVSAPRAQPDRPVRRKTPEPSA
                     PRTGQFRWLTHGLALAIGLAIGIAIGYGIPRSMSAAPAAGRGPSQTTDGEMRLPPGHP
                     DISTMMKGGRAAPAMPMGDD"
     gene            complement(2126..3196)
                     /locus_tag="PLQ54_03405"
     CDS             complement(2126..3196)
                     /locus_tag="PLQ54_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOS92334.1"
                     /translation="MRRMAVLVVALALALAIGAAGCSKPQQEGTPAGPSATQTKMPTD
                     TGKKGTAANPWVIGMSQCNLGEPWRIRMNDDIKKADEATPQVTVQFKDAQGKEDQQQA
                     QVQEFIDAKVDLLIISPKEAIPLTAPVEKAMDAGIPVIVLDRKIASDKYTCFIGADNT
                     AIGREAGKYLVKLLGGQGNVVELRGLETSTPAGERHRGFVQGIAGSGLKIIASKDCQW
                     FEDVAQKEMATILSRYDKIDAVYGHNDPSAHGAYVAAEQEGKGREKTIKFIGIDGNVD
                     EGLRYVKEGLLAATFDYPTGGKEAIETAVKILSGEEVPKKITLGTALITPENVDRGGE
                     PIAPLVPPDEPTAAGTSTGPGQ"
     gene            complement(3279..4538)
                     /locus_tag="PLQ54_03410"
     CDS             complement(3279..4538)
                     /locus_tag="PLQ54_03410"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS92335.1"
                     /translation="MDELRPGTEGPGSSSLARRALALAGEYRGPVALVVVLVVGILIG
                     GRTPDGRPVFLTGSTHVSVFFEYAEYGILAAGMTLVILTGGIDLSVGSVLGLSSVIFA
                     ILTLGCGWSSQWAALAAAGVGAVCGLINGILISRLRFQPFVATLAMMVVARGLAKQVT
                     GHRKIAPALDPWYHVRSLSEMPPIYKGMMSPLAKHGIAVTVLVVPALIIVAAAFFRYT
                     VTGRALDARLFGSSPAGRTARATLSIGAWAIALGLTVWGLAPYLQPASFLFVSSLLLM
                     ALVVKVTRFGRHLYAIGGNEEAARLSGVPVARSKVLAYVLCGLFSGLAGIVTTCRLQL
                     GNPEAGATYELDAIAAVVIGGTSLMGGRGSVALTLVGALIMGYANKILSNIQIHGQFL
                     QDGAKLLAKGMIIVLAVLVQEARRRRA"
     gene            complement(4543..>4821)
                     /locus_tag="PLQ54_03415"
     CDS             complement(4543..>4821)
                     /locus_tag="PLQ54_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOS92336.1"
                     /translation="KVARAKWLMTVPSVLLLDEPTRGIDVGAKAEIYELMNTLVAQGK
                     GILLITSELPELLAMSDRILVMHRGRMTAELNRDEATQERIMAAAMEA"
BASE COUNT          737 a         1672 c         1626 g          786 t
ORIGIN      
        1 ggccagccgg gcggcgacgt tggcgtccgg gatctcctgc ttgccgcacc gcatgcagac
       61 gatcgcgtgc acctggtcct cgagcaccac cgggtgccag gcgtggaacc cgatcgcgca
      121 ggtcaacggc ttcatggtcg tggtcgtgac cacggcgcgg cggcccacac cggaactgcg
      181 gagcggcgcg gaggagctgg cgactgagct cgggctcgaa gtggtcgagc gagcgggcga
      241 gagcatcagc gccctgcgcc agcgccacgg agcggacggg gtcctggtcg tggctcacga
      301 cgggatcaca taccgagtgg gagacgtaac cttcttctat caccccagca tggcgaaggt
      361 ccgcgtccgg ggccttcagc ttggggcgcg ggacgtcacc gtcgatgcca tggggctgca
      421 gcccggatgg cgggtgctcg actgcaccct tggccgtgca tccgacgccg ccgtagccgc
      481 gcatgttgtc gggacctcgg gccgggtggt gggactcgaa atcgacccca tcatcgccgc
      541 tctgacgcgg cgaggactgc tgactttcga tccggacagc cgggcgctct cggaggcctt
      601 gcggcggatc gacgtcatct gcgccgacca tgccgactac ctggcccagg cgtcgagccg
      661 cagcttcgat gtggtctact tcgatccgtt cttcgaggag accgtcgagg cgtccggtgc
      721 catgcaagac ttgagggccg ttggatccca tggtgcggtc actgctgaca gtcttgccga
      781 ggccgctcga gttgcgaggc gccgggtcgt tttcaagacg cgcaggggcc tctgcccccc
      841 gctggtggcc gcctggcgcg ccgtggccgg aggcaagagc cgcgtcgagt accgggtgct
      901 cgacatcgaa gagagctagc catgtaccgg cccgggggcc gaaccgtcat cgttcttctg
      961 ggtattgccg tggtggtgct ggccggccgg tgggcgcccc ctccaccccg cggcgagatc
     1021 gcggcgccac gccagaccgt gtgggtcgaa cgcgccgtcg atggtgatac cctccaactc
     1081 cgcggcctcg ggcgagtccg cgtcatcggc gtcgacaccc ccgaagtcgg ggaaccgggt
     1141 tacgcggaag ccaccgagtt cacgcgcaag acctgtgccc ggcgactcgt cgagatcgag
     1201 gtctgcccgg tgagaccggt tgaccggtac gaccgcgtgc gcgcgatggt ctactaccgc
     1261 gatgatgcgg ggcgggagca cagcctggcc gatgagctca ttcgccggcg tctcggccat
     1321 gcgctctcca ttcagccctg ccacatccct gactcccact ggtatgatct gcagtcgcag
     1381 aggccgcctg gggagccgga tggggagtga gcacgatgcg gtgccgcgaa tgtggggcca
     1441 gcctgcctga ggacgccgtc gagtgtgccg aatgtggcgg acgagtcagc gcaccgaggg
     1501 ctcagccgga tcggccggtc cgtcggaaga ctccagaacc ctcggcgcca cggacgggcc
     1561 agttccgctg gctgacgcac ggcctggctc tggccattgg cctggccatt ggcatcgcca
     1621 tcggctatgg catcccccgg agcatgtctg ccgcgcccgc cgcgggacgc gggccgagcc
     1681 agacgacgga cggcgagatg cggctcccac cagggcatcc cgacatctcg accatgatga
     1741 agggcggccg cgccgcgccc gccatgccca tgggcgacga ctgagccggg cccctcgccc
     1801 gagctggcga aggtgaactc gaaagggcac gggcaccgga acggcccctg ggcctcgggc
     1861 ggccagaagc acacgcgaac cttcccctcg ccggtcagcc acgcgttgct gccgaacacg
     1921 agtgtggtct cgttcgctgc ggcgatgcgg gccttcagcg tctgatggtc tcgcgcgatg
     1981 ccgctcgaga gctccagccg cttgcctcgc aggcagcaag cgcagcgctc gcggacggct
     2041 cgcgctcccg tcccatgcag cgcgaacgcc cggccgagca cagggcccgg ccgggcgtcc
     2101 atgtcccaat ccgctcgagc actgcctact gccccgggcc ggtcgacgtg cccgcggccg
     2161 tgggctcgtc ggggggcacg agtggggcga tgggctcgcc gccccggtcg acgttctccg
     2221 gcgtgatgag cgcggtaccc agtgtgatct tcttcgggac ttcctcgcca ctcaggatct
     2281 tgacggccgt ttcgatggct tccttgccgc cggtcgggta gtcgaaggtt gcggccagga
     2341 ggccctcctt gacgtagcgg agcccctcgt ccacattgcc atcgatgccg atgaacttga
     2401 tggtcttctc ccggcccttg ccttcctgct ccgcggcgac ataggcaccg tgggcgctcg
     2461 ggtcgttgtg cccgtacacg gcgtcgatct tgtcatagcg tgacaggatg gtggccatct
     2521 ccttctgggc cacgtcctcg aaccactggc agtccttgga tgcgatgatc ttgagccccg
     2581 agcccgcgat tccctggaca aagccccgat ggcgctcgcc ggcgggggtg ctggtctcca
     2641 gtccgcggag ctccaccacg ttcccttggc cgcccagcag cttcaccagg tacttcccgg
     2701 cctcgcggcc gatggccgtg ttgtccgcgc cgatgaagca tgtgtacttg tcgctcgcga
     2761 tcttccggtc cagcacgatc accggtatcc cggcgtccat ggccttttcg accggtgccg
     2821 tgagcgggat ggcctccttg gggctgatga tcagcaggtc gactttggcg tcgatgaact
     2881 cctgaacctg agcctgctgc tggtcttcct tgccctgggc atctttgaac tgcaccgtaa
     2941 cctggggggt cgcctcgtcg gccttcttga tgtcgtcgtt catgcggata cgccagggct
     3001 cacccaggtt gcactgggac atgccgatga cccacgggtt cgctgccgtg cccttcttgc
     3061 ccgtatcggt gggcatcttc gtctgggttg ccgaggggcc tgccggggtc ccctcctgct
     3121 ggggcttcga gcacccggct gctccgatcg cgagagccag cgcgagcgcc acgaccagta
     3181 ctgccattcg tctcatcggg gtacctccat ggtgcccgtc cgtggggggc gatcggaccg
     3241 tgagaggccc gcagccgttg tgttcctcac ccgacgagct aggcccttct gcgccgggct
     3301 tcctgaacca gcacggccag gacgatgatc atgcccttgg ccagcagttt cgccccatcc
     3361 tggaggaact gcccgtggat ctggatgttg ctcaggatct tgttggcgta gcccatgatg
     3421 agcgcgccca ccagcgtcag cgccacgctg ccgcgcccgc ccatcaggct ggtgccgccg
     3481 atgaccacgg ccgcgatggc atcgagctcg tacgtcgcgc ccgcctcggg gttcccgagc
     3541 tgcagccggc aggtcgtcac gatgccggcg aggccggaga acagcccaca caagacgtag
     3601 gccaggacct tggacctcgc caccggcacg cccgacagtc gggcggcctc ctcgttgccg
     3661 ccgatggcat acagatgccg cccgaaccgc gtcaccttca ccaccagggc catgaggagc
     3721 agcgaactca cgaagaggaa cgaggcgggc tgcagatacg gggcgagccc ccagaccgtg
     3781 aggcccaggg ctatggccca cgcgccgatc gacagcgtcg cgcgagccgt gcggccggcg
     3841 ggagacgagc cgaacagcct ggcgtcgagg gcacggccgg tcacggtgta cctaaagaag
     3901 gccgcggcca cgatgatcag cgccgggacc accaagacgg tgaccgcgat gccatgcttg
     3961 gccagagggc tcatcatgcc cttgtagatg gggggcatct cggacagcga ccggacgtgg
     4021 taccacgggt cgagcgccgg agcgatcttc cggtggcccg tgacctgctt cgccaggccc
     4081 cgggctacga ccatcatggc gagcgtcgcc acgaagggct gaaagcgcag ccgggagatc
     4141 agaatgccgt tgatgagccc gcacacggct ccgacgccgg cggcggccag ggccgcccac
     4201 tggctcgacc agccacaccc gagagtgagg atggcgaata tcaccgagct gagccccagc
     4261 accgagccga cggacaggtc gatgccgccc gtcaggatga ccagcgtcat gccggcggcc
     4321 aagatgccgt actcggcgta ctcgaagaac acgctgacat gggtcgagcc ggtgaggaag
     4381 acggggcggc cgtcgggagt cctgccgccg atcaggatgc cgacgaccaa caccacgacc
     4441 agcgccactg ggccccggta ctcgcccgcc agcgccagtg cgcgccgggc cagactgctc
     4501 gaaccggggc cctcggtccc cggcctcagc tcatccatcg actcaggcct ccatcgccgc
     4561 cgccatgatg cgctcctggg tcgcctcgtc ccggttcagc tcggccgtca tccggccgcg
     4621 gtgcatcacc agaatccggt cgctcatcgc cagcagctcc ggcagctccg aggtgatcag
     4681 cagaatcccc ttgccctgag cgaccagcgt gttcatcagc tcgtagatct cggccttggc
     4741 gcccacatcg atcccgcgcg tgggctcatc gagcagcagc acggagggca cggtcatcag
     4801 ccacttggcg cgagccactt t
//
LOCUS       DAOSPL010000121         4814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_17714_length_4814_cov_3.060097, whole genome shotgun sequence.
ACCESSION   DAOSPL010000121 DAOSPL010000000
VERSION     DAOSPL010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17714_length_4814_cov_3.060097"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PLQ54_03420"
     CDS             <1..602
                     /locus_tag="PLQ54_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92337.1"
                     /translation="RHRVDQPFVVEPFDPADLSIPRERVREHSREWQRLRTRYYTEGL
                     RVHVGKDSSDPATWIPEYWNDTHALMAAIELAGARNAMMWYIGISGQLGVTASQDPPT
                     YTTPIETMEAYYEELKAGPWVALAWWVFGDWSRWCQGGLRYYDRTLLHYTPEHPEGEP
                     YSPEMLDYWHDEYVAMKLRMFHDVVYHQFGGLNGPAPSE"
     gene            642..1589
                     /locus_tag="PLQ54_03425"
     CDS             642..1589
                     /locus_tag="PLQ54_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS92338.1"
                     /translation="MEYRKLGQTGVDVSAMVLGAWQYGQAYWKGIDDSESVATIHAAL
                     DAGINMIDTAVGYGDGYSETIVARAVGNRRGDVLFASKCWGDPTTIPQTIDGCLQRMG
                     TDCIDLYQVHYPSPRYPVCETIGAMAEIQKAGKIRWIGVSNFSLEQMREAVATARIET
                     CQPPFNVFWRQIDDDVLPFCRENDIAVLAYSPLAQGLLAGRFRSVADVPDDIRARSKL
                     LAPDILERCLPTVDRLAQIAAAHGKSLPQAALAWALAVPGITCTIVGARRPSQLQENL
                     GGIGWSLTPEEQEEISEAGLEVSRLLDFSSNMWGWSPAT"
     gene            1660..2460
                     /locus_tag="PLQ54_03430"
     CDS             1660..2460
                     /locus_tag="PLQ54_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92339.1"
                     /translation="MRVQPVRRYTDPRYPAASALDTHPELLRLLPRRWRSNSLVLAGL
                     AATGGLLLAGCRPSWADTFEGELKVAPIFTHGDGRGAFGCVAVNPPVFLSEEEARGVI
                     IEEAKTAGIVFETDVLTLPEVRVPETTENGSVLPDEGEEPRTKVRELKLDGTDRERRI
                     SFEYVSQDDYEAWKADDGGPMSTVSSFDLIGTAKVLRDGLAVAHAAEDGTVAVLYDPM
                     ERYLEDTDQVAGGVDWQQAEKDAKERAKAQLRKQVKGFIEWLKAEGVI"
     gene            2462..3622
                     /locus_tag="PLQ54_03435"
     CDS             2462..3622
                     /locus_tag="PLQ54_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS92340.1"
                     /translation="MHLTLHLTARCNLRCRYCYAAPHAGGDMMPEVLRAAIDMAVAMN
                     QRERPGLSLGIVFFGGEPLLRRDLIHQALAWCREIETSTGQLFHYKITTNGTLLDEEF
                     LTSPETRDVFVAMSHDGLRESHDAHRVDAAGQGTFDRLEPVIDRLLARRPYAPVLLVT
                     NPDTVQYFANSVRYLHRRGFTYLITTPNYGAEWDERSLAELRRQYQSLGGWYYEAMMR
                     EEKFYFSPFEVKIGSHVRPGSCKAERCELGRTQISVAPNGKLYPCVQFVGDGTDDEYA
                     IGDVFSGIDAEARWRLYAANGEEKESCAECAIRDRCNHYCGCLNRQATGRIDRVSPVL
                     CAHERIVIPIADRVAERLFKKRNALFIHKHYNELYPIVSLLEDTSRARADMR"
     gene            complement(3723..4421)
                     /locus_tag="PLQ54_03440"
     CDS             complement(3723..4421)
                     /locus_tag="PLQ54_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOS92341.1"
                     /translation="MPTRCASPAAVPQPGGGVPRQRRNILARSGVGHLRLVDGDLVQP
                     SNINRQLMALESTLGRPKVDVAAARVVDINPQCAVEPLRLFVDADSLGPVLTAPLDLV
                     VDAIDSAGPKLELIAAALRSGIPIVSSMGAALRVDPTRVRTGVIADVQGCPLARRLRR
                     GLRGRGLGLDVPCVFSEEPATRAWIGPSEDPQGPGPTARRRALGSLPTVTGTFGLVLA
                     ALALRMLSVERPGP"
     gene            4420..>4814
                     /locus_tag="PLQ54_03445"
     CDS             4420..>4814
                     /locus_tag="PLQ54_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOS92342.1"
                     /translation="MHLGPRAESGGNRNPSAWLCGDFAALPSAAPTPADPVPSGSLFR
                     RHLYRDFHEAELQVILRNYDRLSMAHGVEVRMPLMDWRLVTYAFALPPNSLLGHGYSK
                     RILREAMRGRVPDVVLERRDKIGFASPVSA"
BASE COUNT          840 a         1573 c         1660 g          741 t
ORIGIN      
        1 gccgtcaccg cgtcgatcag cccttcgtcg tcgagccatt cgacccggcc gacctgagca
       61 tcccgcgtga gcgtgtgcgg gagcactcgc gcgagtggca acggctgcgg acccggtact
      121 atacggaggg cctgcgggtc cacgtgggca aggacagcag tgatccggcc acgtggatcc
      181 ccgagtactg gaatgacacg cacgcgctca tggccgccat tgaactggca ggcgcccgaa
      241 acgcgatgat gtggtacata gggatctcgg gccagctcgg cgtaacggcg agccaggacc
      301 cgccgacgta cacgactcca atcgagacca tggaggccta ctacgaggag ctgaaagcag
      361 ggccttgggt agcgctggcc tggtgggtgt ttggtgactg gagtcgatgg tgccagggcg
      421 gccttagata ctacgaccgc acgctgctgc actacacgcc cgagcatccc gaaggcgaac
      481 cctactcacc ggagatgctc gactactggc acgacgagta cgtggccatg aagctacgca
      541 tgttccacga cgtggtctac caccagttcg gaggtctgaa cggaccggcg ccgtccgagt
      601 gatggcggcg ggacgctgcg agagggacga ggtgcaaggc aatggagtac cgcaagttgg
      661 gccaaacagg cgtcgatgtg tcggccatgg tcctgggcgc gtggcagtac ggccaggcct
      721 actggaaggg cattgacgac agcgagtcgg tggcaaccat ccacgccgcg ctcgatgccg
      781 gcatcaacat gatcgacacc gccgtgggct atggcgacgg ctactcggag acaatcgtgg
      841 cccgggccgt gggcaaccgg cggggcgacg tgctgttcgc cagcaagtgc tggggcgatc
      901 cgaccacgat cccgcagacc atcgatggat gcctgcagcg catgggcacc gactgcatcg
      961 acctctatca ggtgcactac ccgagcccca ggtaccccgt ctgtgagacc atcggggcta
     1021 tggcagagat ccagaaagcc ggcaagatcc gctggattgg cgtgtcgaac ttctcgctgg
     1081 agcagatgcg ggaagccgtg gcgacggcgc ggatcgagac ctgtcagcca ccgttcaacg
     1141 tgttctggcg gcagatcgac gacgacgtgc tccccttctg ccgcgagaac gacatcgcgg
     1201 tgctggcgta tagccctctg gcccaagggc tcttggcggg gcgtttccgg tccgtggccg
     1261 atgtgcccga cgacatccgt gcccgcagca agctgctggc gcccgatata ctcgagcggt
     1321 gccttcccac ggttgaccgg ctggctcaga tcgcggccgc ccacggcaag tcgctgccgc
     1381 aggccgcctt ggcatgggcg ctggctgtgc cgggcatcac ctgcacgatc gtgggtgccc
     1441 ggcgcccctc gcagctccag gagaacctgg gtggcatcgg gtggagcctg acaccggaag
     1501 agcaggagga gatcagcgag gcagggctcg aggtgagccg cctgctcgac ttcagcagca
     1561 acatgtgggg ctggagtccg gcgacgtagg acgcgggtgg aaccaatccg tggcgccgag
     1621 ggtccaacct cgtgtccgta gccagggggg agtgtcccga tgcgtgtgca accggtccgc
     1681 cgctacacgg acccgcgcta cccggccgcg agcgcgctcg acacccatcc cgagctgcta
     1741 cggttgctgc cccggcggtg gcggagcaac tccctcgtgc tggccgggct ggccgctacc
     1801 ggcgggttgt tgttggcggg atgtcggccc tcttgggccg atacgttcga aggagagctg
     1861 aaggtggcgc ccatcttcac gcacggcgat gggcggggcg ccttcggttg cgtcgccgtc
     1921 aatcctccgg tgttcctgag cgaggaagag gcccggggcg tcatcatcga ggaagcgaaa
     1981 acggcgggca tagtcttcga gaccgatgtg ctcacgttgc ccgaggtgcg cgtgccagag
     2041 accacggaga acggctccgt cctgccagac gagggggagg agccccggac caaggtgcgc
     2101 gagctcaagc tcgatggcac cgatcgcgag cgccgcatct ccttcgagta cgtctcgcag
     2161 gacgactacg aggcctggaa ggcggacgac ggcgggccga tgtcgaccgt gtcgagcttc
     2221 gacctgatcg gcacggccaa ggtcctgcgc gatgggctcg ccgtagccca cgccgcggaa
     2281 gacggcacgg ttgccgtcct ctacgacccc atggagcgct acctcgagga cacggaccag
     2341 gtggccggcg gggtcgactg gcagcaggcc gagaaggacg ccaaggaacg cgccaaggcg
     2401 cagctgcgga agcaagtgaa gggcttcatc gagtggctga aggccgaagg cgtcatctga
     2461 cgtgcatctg acactgcatc tcacggctcg ctgcaaccta aggtgccgct actgctatgc
     2521 cgcgcctcac gccggcggag acatgatgcc cgaggtactc cgggccgcga tcgacatggc
     2581 cgtggcgatg aaccagcgcg agcggccggg gctgagcttg ggcatcgtct tcttcggcgg
     2641 cgagccactg ctgcggcgcg acctgatcca ccaggccctg gcctggtgcc gcgagatcga
     2701 gaccagcacg gggcagctct tccactacaa gatcacgacc aacggcacgc tgctcgacga
     2761 ggagtttctc acgagcccgg agacccgcga cgtgttcgtc gccatgagcc atgacgggct
     2821 gcgcgagtcc cacgacgccc accgggtcga cgccgccggc cagggcacct tcgaccggct
     2881 tgagccagtg atcgaccgcc ttctcgcccg gaggccctat gcgccggtcc tgctcgtcac
     2941 gaacccggac acggtgcagt acttcgccaa ctcggttcgc tacctgcacc gtcgtgggtt
     3001 cacgtacctg atcacgaccc caaactacgg ggccgagtgg gatgagagat cgctggccga
     3061 gctgaggcgg cagtatcagt ctctcggcgg ctggtactac gaagccatga tgcgcgagga
     3121 gaagttctac ttcagcccgt ttgaggtgaa gatcggctcg cacgttcggc cggggagctg
     3181 caaggctgaa cggtgcgagc tgggccgcac gcagatctcc gtggcgccga acggcaagct
     3241 ctatccgtgc gtgcagttcg ttggcgacgg gactgacgac gagtacgcca tcggcgacgt
     3301 gttctcgggc atcgacgcgg aggcgcgctg gcggctctat gccgccaacg gcgaggagaa
     3361 ggagtcgtgc gccgagtgcg cgatccgcga ccggtgtaac cactactgcg gatgcctcaa
     3421 ccgccaagcg acgggccgca tcgaccgcgt gtcgccggtc ttgtgcgccc acgagcggat
     3481 cgtgataccc atcgccgacc gggtggcgga gcggctcttc aagaagcgca acgcgctgtt
     3541 catccataag cactacaacg agctgtaccc catcgtgtcg ctgctcgagg atacctcgcg
     3601 ggcaagggcg gacatgcggt aggcgagcgt ccgccttgcc cctgcgcgtc gctcctcagg
     3661 gccgacggaa gaggggggcg acccgtctgc cgggccacaa cgaaagcacg ggctctgctg
     3721 gcctacggcc cagggcgctc cacagaaagc atccgcagcg ccagtgcagc cagcaccagg
     3781 ccgaaggtgc ccgtcactgt gggcaggcta ccgagcgccc gacggcgagc cgtcgggccc
     3841 gggccttgcg gatcctcaga aggcccgatc caggcccgtg tggccggctc ctcactgaac
     3901 acgcagggca cgtcgaggcc cagcccgcgg ccgcgcaagc ccctgcgcaa ccgccgggcc
     3961 aaggggcacc cctggacgtc ggcgatcacg ccagtacgca cacgcgtggg atcgacccgc
     4021 agggcagcgc ccatcgagga cacgatgggg atcccactcc gcaaagccgc cgcgatgagc
     4081 tcgagcttgg gcccggccga gtcgatggca tcgacaacga ggtcgagtgg ggccgtgagc
     4141 accggcccca ggctatccgc gtcgacgaac agccggaggg gctcgaccgc gcactgaggg
     4201 ttgatgtcga ccacacgcgc ggccgcgacg tcgaccttgg gtcggcccaa cgtcgactcc
     4261 agcgccatga gctgccggtt gatgttgctc ggctgcacga ggtcaccgtc gaccagtctc
     4321 agatggccga caccggaccg cgccaggatg ttacggcgct ggcgtgggac acctccacct
     4381 ggctgcggaa cagccgccgg gctcgcgcaa cgggtaggca tgcacctcgg tccccgggcg
     4441 gagtcgggtg ggaaccggaa tccgtccgcc tggctatgtg gcgactttgc tgcgctcccg
     4501 tcagctgcgc ctactccggc cgatccggtc ccgtccggtt cgctgttccg gcggcatctg
     4561 taccgtgact tccacgaagc ggagctccag gtgatcctgc gcaactacga ccgtctctcc
     4621 atggcgcacg gcgtcgaggt gcggatgccg ctgatggact ggcggctcgt gacctatgcc
     4681 tttgcactgc cgcccaacag cctgttgggg catgggtata gcaagcgcat cctgcgggag
     4741 gccatgcgcg gccgcgtgcc ggatgtcgta ttggagcgcc gggataagat cgggttcgct
     4801 tcgcccgtct ccgc
//
LOCUS       DAOSPL010000122         4801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_17772_length_4801_cov_3.596502, whole genome shotgun sequence.
ACCESSION   DAOSPL010000122 DAOSPL010000000
VERSION     DAOSPL010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17772_length_4801_cov_3.596502"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PLQ54_03450"
     CDS             <1..395
                     /locus_tag="PLQ54_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-dependent phosphohydrolase"
                     /protein_id="HOS92343.1"
                     /translation="CGATINYSVPGRLLGHIAIGFSEVDRRARAIPDFPEELRLQLLH
                     IILAHHGSSEFGSPVRPMTAEAVAVHCIENCDAQVAQFEEKIGAAVEEGREFTEYDPL
                     LERYLYAGSRAVRDPGKRIGANGQVELF"
     gene            complement(407..1657)
                     /locus_tag="PLQ54_03455"
     CDS             complement(407..1657)
                     /locus_tag="PLQ54_03455"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOS92344.1"
                     /translation="MKVIELLSPHVHSVCYSLHVGAGSLTDPPNRPGVASLTQRVVIE
                     AETMARRRSAPHGRMCYGGVDVGQAYVTVWGHRSRGEELARLVPQVLADAEFTEEALL
                     REKSRLWDTLPDTNDGWTDTTDLVNRLLLAPGLNWNVFGSRESVESITVADVATSFNE
                     HWLGSDMVLAVAGAYSREDLEQAVGELSTLVASAGSSNTAPFRGGVEAPRRAAVVPVP
                     DERFSLHLLFPGVSVLHSDAFLIRLLDCIVGGTPDARVPKRLAWDLGLADASYSMVFF
                     VNDQSILTVYVEGIYERFHEILRALVRSVESLTFEPPTKAELQEAQTVLAFERDYQLD
                     DPQTAATARGSLYFASGMDDPLAFEQLLIGEPTATRDQVFEAAQRVLSWDNLSVLYVG
                     PEVEEVVSLVQSTWPYKPIEIWGE"
     gene            complement(1668..2396)
                     /gene="rpoD"
                     /locus_tag="PLQ54_03460"
                     /pseudo
     CDS             complement(1668..2396)
                     /gene="rpoD"
                     /locus_tag="PLQ54_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
     gene            complement(2553..3914)
                     /locus_tag="PLQ54_03465"
                     /pseudo
     CDS             complement(2553..3914)
                     /locus_tag="PLQ54_03465"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(3904..>4801)
                     /locus_tag="PLQ54_03470"
     CDS             complement(3904..>4801)
                     /locus_tag="PLQ54_03470"
                     /inference="COORDINATES: protein motif:HMM:NF021895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92345.1"
                     /translation="VLPAGEDPDSLLRERGPSAFDAVVAEAVPLVHFAIDHIVASRAS
                     EGVAGRERAAEEIADLLAAIRKPVARSGYIAYAASRLAGSDPGRVASLERALGEQLRL
                     SERKRLRAHSGAAQGESSRTVARPVDPRRDRGFVVESVSQGATAELRPAVVQAERVLL
                     RSALADRGHAERIFRELPPDRFLRPVSREIARELQRAWASGAAVAGAEILSALSAAAA
                     EEFGEVVLPGSIHEAEGLPLQQLDLVRDHWEEQRFRELRERVARIPPEEQRTSDDVRE
                     FMELVRRRHAGPRGRFSPADGG"
BASE COUNT          781 a         1660 c         1554 g          806 t
ORIGIN      
        1 agtgcggtgc cacgatcaac tactcggtcc cggggcgcct gctcggacac attgccatcg
       61 ggttctccga ggtcgaccgc cgggcccgcg ccatcccgga tttccccgag gagctgcgcc
      121 tgcagctcct gcacatcatc ctcgcccatc acggctcctc ggagttcgga tcgccggttc
      181 gcccgatgac tgccgaggcc gtggccgtcc actgcatcga gaactgcgac gcccaggtgg
      241 cgcagttcga agagaagatc ggcgctgcgg tcgaggaagg gcgcgagttc accgagtatg
      301 acccgctgct cgagcggtac ctctacgccg gcagccgggc cgtgagggat ccgggtaaac
      361 ggatcggcgc caacgggcag gtcgagctgt tctgacggcg gggccgctac tcgccccaga
      421 tctcgatggg cttgtagggc caggtgctct ggaccaggga cacgacctct tcgacctcgg
      481 gacccacgta caacacactg aggttgtccc acgaaagcac acgctgcgcg gcctcgaaaa
      541 cctgatcacg cgtggccgtg ggctcgccaa tcaggagctg ctcgaaggcg agtgggtcgt
      601 ccatcccgct ggcgaagtag agcgaccccc gagccgtggc ggcggtctgg gggtcgtcga
      661 gctggtagtc acgctcgaag gccagcacgg tctgcgcctc ttgcagctcc gccttggtgg
      721 ggggctcgaa ggtcaggctc tcgacgctcc gcaccagagc tcgaaggatc tcgtggaagc
      781 gctcatagat cccctcgaca tagacagtga ggatgctctg gtcgttgacg aagaacacca
      841 tcgagtagga ggcgtcggcc aagcccaggt cccatgccag ccgcttgggc accctggcat
      901 cgggggtgcc tccgacgatg cagtcgagca gccgaatgag gaatgcatcg gagtgaagaa
      961 ccgagactcc cgggaacagc agatgcaggc tgaaccgctc gtctggaacg gggacgacgg
     1021 cggctcgcct gggcgcctcg acgccccccc ggaacggcgc ggtattgctc gaaccggccg
     1081 aggcaaccag cgtcgacagc tctccgacgg cctgttcgag gtcctcgcgt gagtaggcgc
     1141 cggccacggc aaggaccata tcactcccca gccagtgctc gttgaacgat gtggccacgt
     1201 cggccaccgt gatgctctcg acggactcgc gggagccaaa cacattccag ttcagtcccg
     1261 gagccagcag caggcggttg accaagtcgg tcgtgtccgt ccacccgtcg ttggtgtcgg
     1321 gcagggtgtc ccagaggcgc gacttctccc ggagcagggc ctcctcggtg aactcggcat
     1381 cggccagcac ctgaggcacg agccgcgcca gctcctcacc gcgggaccgg tggccccaga
     1441 ccgtcacgta ggcttgtccc acgtcgacgc cgccatagca catgcggccg tgaggcgccg
     1501 agcggcgacg cgccatggtc tccgcctcga tcaccaccct ttgggtgagc gaagccacac
     1561 ccgggcgatt gggcggatcc gtaaggctac cagcgcccac atgcagcgag taacacacgg
     1621 aatgcacgtg gggcgagagg agctcgatga ccttcatgct gggtcgctca atccaggtag
     1681 tccctcagac ggcggctgcg ggccgggtgg cgcagtttct tgagcgcctt ggcctcgatc
     1741 tggcggatgc gctcgcgggt cacctcgaac acctggccca cttcctccag tgtccgattg
     1801 tagccgtcca ccagaccaaa gcgcagcttc agcacctcgc gctcgcgctc gctgagggtc
     1861 gataggacct tctcgagctg ctcccgcagg acttggttcg atgcggcatc gaccggtgag
     1921 tgaacctctt ggtccacgat gaagtcgccc aggtagctgt tctcatcctc gcccacgggg
     1981 gcttcgagcg aaaggggctc gggcgcgatc cggatgatct cgcccacccg ctcgacgggg
     2041 aggtccatct cctcggccag ctcctccagc gtcggttccc gcccgagctg ctgaagaagt
     2101 tgtctagatg tctttacaag tcggttgatg gtctcgacca tgtgcaccgg aatacggatg
     2161 atgcggccct ggtcggcgat ggcgcgggtg atggcctgcc ggatccacca ggtagcatat
     2221 gtgctgaact tatagccttt gcggaagtcg aacttctcga ccgcccgcat gaggcccatg
     2281 ttgccctctt ggatcaggtc caggaaggac agcccacctc gtcccgtgta cttcttggcg
     2341 atgctcacca ccaggcgcag gttggcgtcg gccaggcgat ggatcgcgtc ctggtccttg
     2401 agcttgatgg tgtcctccga cacgctggtc gggtccatct tctgatcgaa gcagatctgg
     2461 atctggaggt cgacccggac ccctcgctcg ccgggacggg gccagatcca gaagatcttc
     2521 ggcttcctgg gccggctgcc gacggtgaag gtccaggtca cgtcctcctc aaggcggacg
     2581 ccgtagacgg tgcacacgcc gcgatcgccg ccgctgaccc gggccgtgta ctcctcgccc
     2641 gcctccaggg ccgccttggg cacgaacacg gccgcccggc cgtcgcaggt gagctgcacg
     2701 gtgccctgga cgcgtttccc cctcggcccc gtgagcgaga aggtacgaga cgacaccgag
     2761 ctcgaggtca tcgactcggc gaacttgacg cccactatgg cctgctgagg gacatgccga
     2821 tcgccctcaa tgggcgtccg gtgcgtgatc agtgggtcgt gggcgccgcg gaccccaaat
     2881 cggatgacga catcggcgtc gagcaggcgt ccgtcggtac cccggacccc gcggggcccg
     2941 ccccgcacgc gcaaccgata gctccggccg gtctcaagcg cgcgcttggg cttgacggtg
     3001 acggtggctt tgccctcgca cttgagcgtc gatggcagca cttgccggtc ggcgctctgg
     3061 agctgcacgg tgtgttcggt aacgctgccc gggtcgaggg ctgacgagaa cgtgatctcg
     3121 aaggccttgt gctgggcggg gatgatcttc tctcgctgtc gtgggcgcgt gcccaccacc
     3181 gagaaggggg actctcccga tccggtgcgg aactccatca catagtcatc ggcgaaggct
     3241 gaccggccgt cgagagcggc gccggcatcc agaccaggtg ggtccagagc gcgaacgcca
     3301 ttctcccccc cctcgatggt cagggtgtag gtgatcccgc cctcgaggcg cgccttgtgt
     3361 gaagcccggg gcgccgcgac aatgatgccg gtgcgggtgg tgtactcaac atcgcactcg
     3421 gcccgctgga tgcgccgggc gaggttgagc tcttcctctg gagtaagaag ggaaaccttg
     3481 ccgatctcgc gcaagtacat gcgcaccgag tcgttgatcg gaatgccctc ggccgcgtcg
     3541 aactcgtcgt cgcgatcgag cttgcggccg atgccgacct tgctcgcgac tgggccgacc
     3601 gtctcctcgc cggcctcctc ggcatccacc agctcgacac cctcgtcctg aaagagctgg
     3661 aggatatcct ccacctgttg gtcatcggcg ccctcgagga ctttcccgag ggagtcgttg
     3721 atctcttcgt agctgaggac gccatcctcg cggcccttgt ccagaagtgc ctggacttcc
     3781 ttgctgttcc tcaggtcctc tttgtccaac gccaatcctt ccgcgtcctc gcgcggccgc
     3841 ggtcgatgcc atcgccccgc tgatccggcc gaggcgtctc gtgagtgcag gtgtgtcctc
     3901 accctaaccc ccatcggcgg gcgaaaaacg accccgcggc cccgcgtggc ggcgacggac
     3961 gagctccatg aactccctga cgtcgtcgct tgttctctgc tcctcggggg gaatcctcgc
     4021 gacccgctcc cgcagctccc ggaaccgctg ttcctcccaa tgatcgcgaa ccaagtcgag
     4081 ctgctggaga gggagacctt cggcctcgtg gatgctcccc ggcaggacaa cctccccaaa
     4141 ctcctcggcc gcggcggcac tgagcgccga gaggatctcg gccccggcaa ccgcagcgcc
     4201 gctggcccag gctcgctgca gttcgcgggc gatctcccgc gagaccggtc gcaggaaccg
     4261 atcgggcggg agttcgcgga agatccgttc cgcatggccg cggtcggcca acgccgaccg
     4321 caagagcact cgctctgcct gcaccaccgc cggccgcagc tctgccgtgg cgccctgaga
     4381 caccgactcg acgacgaagc cccggtcgcg cctggggtcg accggccgcg ccacggtccg
     4441 tgagctctcg ccctgcgcgg ctcccgaatg cgcccgcagg cgcttccgct ccgacaggcg
     4501 gagctgctca cccagagctc gctccaggct ggccacccgg ccggggtctg aaccggccag
     4561 acggctcgcc gcataggcta tgtagcccga gcgagccacc ggcttgcgga tcgccgccag
     4621 cagatcggcg atctcctcgg cggcacgctc ccggcccgcc accccttcag acgcccgaga
     4681 cgcgacgatg tggtcaatgg cgaagtgcac cagaggcacc gcctcggcca cgaccgcatc
     4741 gaacgctgag ggaccgcgct cccgcaacaa ggagtccggg tcctccccgg cgggcagaac
     4801 c
//
LOCUS       DAOSPL010000123         4790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_17823_length_4790_cov_2.983316, whole genome shotgun sequence.
ACCESSION   DAOSPL010000123 DAOSPL010000000
VERSION     DAOSPL010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17823_length_4790_cov_2.983316"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLQ54_03475"
     CDS             <1..251
                     /locus_tag="PLQ54_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HOS92346.1"
                     /translation="MEIIDEVERTRRGPYTGSAGYLAYDGDMDLNIIIRTFVIKNGVA
                     YAQVGGGVVADSDPESEFEETLHKARALLRAAQVAEEG"
     gene            248..1111
                     /locus_tag="PLQ54_03480"
     CDS             248..1111
                     /locus_tag="PLQ54_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOS92347.1"
                     /translation="MSLVFLNGALVPETEARVSVRDRGFLYGDSVFETLPVYSGRVFL
                     LAEHLSRLVASASRILLPLPWTVEELAQSVTVTIGANSLDRGSLRLTISRGTGPPGID
                     PLAHCQPTLVIAPAPARDHTTDRERGVAVVISSVPKASAEAIPADAKAGNWLNHILAR
                     RDASEAGAFEALLLHADGSVAEGTVSNAFAVRGGTVATPPADGRILPGITRWAVLRLL
                     AELGIPAEERVLLPDDLHAADEVWITNSLIEILPACSLDGEPLASPWPLCRHVQQAYT
                     DWVRRECSGGR"
     gene            complement(1170..2120)
                     /locus_tag="PLQ54_03485"
     CDS             complement(1170..2120)
                     /locus_tag="PLQ54_03485"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOS92348.1"
                     /translation="MVHMLCVAAAVWLASGAAPDGAAVDTGSGWDVSWGGHPVFSYRF
                     SDRLMKPYIHPLLTPRGHAVTLDSPADHIHHHGLMFGWGNVQPATEGRDYHLVFFGEE
                     GDPHTIGRILPDPELPPKVEVADHAVRIVTHNQWRRLSDGLLVLTERREIISHRPRPG
                     RGNLLTWITEQRAEVDLSLGPTPGRDVSYYGLGLRTPRDMRWALFANSNGKRGTPACY
                     GDRADWCACGTTVEPARGFAMFDHPDNPRHPTGYFVMDNDFSYMTASFVSHEPYPMRK
                     GDVLRLCYGVLAFDGDIDAGAIADEYDRWIRLTVGRKRAR"
     gene            complement(2153..3148)
                     /locus_tag="PLQ54_03490"
     CDS             complement(2153..3148)
                     /locus_tag="PLQ54_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS92349.1"
                     /translation="MGGIGLQMRPLGQSGIQASAIGLGTWAVGGWMWGGSEEAECIRA
                     IQASLDTGVNLIDTAPMYGYGRSEEIVGKAIRGRRDQVVLATKCGLVWWDTRGTHFFD
                     KDGNAIYRYLGPDSIRHEVEQSLTRLGVDHIDLYQTHWQDDTTPREDTMSVLLDLKAQ
                     GKIRAIGVSNVQVAQLKEYQAVGAVDSAQEQFSMLDRQIEAELLPHCRATNVAVLAYS
                     PLALGLLSGRIGPDRKFEGDDLRQGNPRFSPENLAKVAAMLEEMRPVADSRSLTLAQL
                     VIAWTIAQPGLTHALVGARRPEHAAENAVPGDVVLSDDELAVINGVIERHRPGLA"
     gene            complement(3333..4052)
                     /locus_tag="PLQ54_03495"
     CDS             complement(3333..4052)
                     /locus_tag="PLQ54_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92350.1"
                     /translation="MRRWIIPVLVVAVGLSAWIAQGQPGGPGGPGGFGGPGGPMANMA
                     FGTISAVDMEAGIIAVQMGGGGGFGGGPGGGQGFTRQVVLTQDTKVLEQQPATTDEIT
                     EGLEITVVGVPSSLQANQATIADAGTDLSVGFGFGMGFGMGRRGQRGGGPGANMPQAM
                     GNATGKVVGVDPVRIDVGNGVIVEMTTSAQTNLTKLVIAPDEALKVGANVMCQGQGQD
                     DGSLVADTVRLMPAMPGRRGN"
     gene            complement(4123..>4790)
                     /locus_tag="PLQ54_03500"
     CDS             complement(4123..>4790)
                     /locus_tag="PLQ54_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020616606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS92351.1"
                     /translation="KSTIVKLLARHYDPKAGAVRVNATDIRDVALESLRGQMAIVQQE
                     THLFSGSIRDNIRYGRPDATDEQVVEAARIANAHEFISDLPRGYDTDVQERGLRLSNG
                     QRQLISFARAVLASPAVLILDEATSSVDPLTEKRIRDALRVLLHDRIAFMIAHRLSTV
                     SESSQILVIDEGRVVDRGRHSELIDRCDLYRTLYHRRFREDDTEQTLLPAPATEGPTR
                     TEP"
BASE COUNT          808 a         1734 c         1472 g          776 t
ORIGIN      
        1 gcatggagat catcgacgaa gtcgagcgca cccgccgagg gccctacacc gggtcggcgg
       61 gctacctcgc atatgacggc gacatggacc tgaacatcat catccggacc ttcgtcatca
      121 agaacggcgt cgcctatgcc caggtcggcg gaggggtcgt ggccgactca gaccccgagt
      181 cggagttcga ggagaccctc cacaaagctc gggccctgtt gcgcgcggct caggtggccg
      241 aagagggatg agcctcgtct tcctcaacgg cgccctggtg ccggagaccg aggcccgtgt
      301 gtcggtaaga gaccgaggct tcctctatgg cgacagcgtg ttcgagacac tgcccgtcta
      361 ctcgggccgg gtgttcctgc tcgccgagca cctcagtcgg ctcgtcgcgt cggcgagccg
      421 catcctactt ccgctcccct ggaccgtcga ggaactggcc cagtctgtca cggtgaccat
      481 cggcgccaac tcgctggacc gtggttctct gaggctcacc atctcccgtg ggaccggccc
      541 gccgggaatc gaccctctgg cccactgcca gcccacactc gtgatcgccc cggcccccgc
      601 ccgcgaccac accaccgacc gggagcgggg ggtcgcggtc gtcatctcat ccgtcccaaa
      661 ggcatcggcc gaggccatcc cggccgacgc caaggccggc aactggctca accacatcct
      721 cgcccgacgc gacgcttccg aagcgggcgc cttcgaggcc ctactgctcc acgccgacgg
      781 atcagtggcc gagggtaccg tctccaacgc attcgccgtc cggggtggaa cggtagccac
      841 cccgcctgcc gacggccgca tcttgcccgg catcacccga tgggccgtgt tgcgcctgct
      901 ggccgaactc ggtatcccgg ccgaggagcg agtgctacta ccggacgacc tgcacgcggc
      961 cgacgaggtg tggatcacga acagcctcat cgagatactg cccgcatgca gcctcgacgg
     1021 cgagccattg gcctccccgt ggcccctctg ccggcacgtg cagcaagcct acaccgactg
     1081 ggttcggcgc gagtgcagcg gcggccgctg accgcggccc ctggcccacg ccgcgccgat
     1141 gcccggcccc ggcggcggcg cgaggcacat taccgggcgc gcttccggcc cacggtgagc
     1201 cggatccagc gatcgtactc atcggcgatg gcgcctgcgt cgatgtcgcc atcgaaggcc
     1261 agcacgccgt agcagagacg gaggacatcg cccttcctca tgggatatgg ctcgtgcgag
     1321 acgaagctgg ccgtcatata gctgaagtcg ttgtccatca cgaagtaacc ggtcgggtgg
     1381 cgtggattgt cagggtggtc gaacatggcg aagcctcggg ccggctcgac ggtcgtgcca
     1441 catgcgcacc agtccgcccg gtctccatag catgccggcg tgccgcgctt cccattggag
     1501 ttggcaaaca gagcccagcg catgtcacgc ggcgtccgca acccaaggcc atagtaactc
     1561 acatcgcgac ccggcgtcgg gccaagcgag aggtccactt cggcccgttg ctcggtgatc
     1621 cacgtcagca agttcccgcg gcccgggcgc ggccgatggg agatgatctc tcgccgctcg
     1681 gtgagcacca gaagcccgtc gctcagacga cgccactggt tgtgcgtgac aatacggact
     1741 gcatggtcag ccacctcgac cttcggcgga agctctggat ctggcaggat gcgcccgatg
     1801 gtgtgcgggt cgccctcctc gccgaagaac acgaggtgat agtcccgacc ctcggtggcc
     1861 ggctgaacgt tgccccaccc gaacatcagc ccgtggtggt ggatgtggtc agcgggcgag
     1921 tcaagtgtca cggcgtggcc tcgcggggtc aggagtgggt ggatgtacgg cttcatgagc
     1981 cgatcagaga agcggtacga gaagaccggg tggccgcccc acgacacgtc ccaccccgaa
     2041 cccgtgtcca ccgcagcacc gtcaggggcc gcacccgagg ccaaccagac cgcagcagcc
     2101 acacacagca tatgcaccat ggatcactcc ccgcgcgggc gagccgcggt ccctacgcga
     2161 gcccgggccg gtgacgttcg atgaccccgt tgatgacggc cagctcgtcg tcgctgagca
     2221 ccacatcgcc ggggacggca ttctcggccg catgctccgg tcgccgcgca ccgaccagcg
     2281 catgcgtcag tccgggttgg gcgatggtcc aggcgatgac cagctgcgcc aatgtcaggc
     2341 tgcggctgtc agccacaggg cgcatctcct cgagcatagc cgccaccttg gccaggttct
     2401 cgggggagaa cctcgggttg ccttgccgca gatcatcgcc ctcgaacttc cgatcgggcc
     2461 caatccgccc cgatagcagg cccagcgcca acggcgaata ggcgaggacc gctacgttcg
     2521 tggcgcggca gtgaggcagc agctcggcct cgatctggcg gtcgagcata ctgaactgct
     2581 cctgcgcgct gtccacggcc ccaacggcct ggtactcctt gagttgagcc acctggacat
     2641 tgctgacccc gatggcccgg atcttgccct gggccttcag gtcgagcagg accgacatgg
     2701 tatcctcgcg gggagtcgtg tcatcctgcc agtgagtctg gtagaggtcg atatggtcca
     2761 cgccgaggcg cgtcaggctc tgctccacct cgtggcggat agagtccggt ccgaggtagc
     2821 ggtagatggc gttgccgtcc ttgtcgaaga aatgggtgcc ccgagtgtcc caccacacca
     2881 gaccgcactt cgtggccagc accacctggt cgcgccggcc gcggatcgcc ttgcccacga
     2941 tctcctccga ccgaccatag ccatacatcg gcgccgtgtc gatgaggttc acgcccgtgt
     3001 cgagtgatgc ctggatggcc cggatgcact cggcttcctc agaccctccc cacatccatc
     3061 cacccacggc ccatgtgcca agtccaatgg ccgaagcctg gatccccgac tgccccaacg
     3121 gacgcatctg caaaccgatt ccccccatct gatgcgcgcg gacgcgctcg aacagccact
     3181 gccttcccca gacagcaaca gactccctcc cggcgttccc gcggagggag tctgttcgcg
     3241 aagagactac acgcaggcgc gcgcccccag ccggcgccgg ccgccccacg ccacctctcc
     3301 cggttcagaa accagtggcc gaccgccgtg cgtcagttgc cccggcggcc gggcatggcg
     3361 ggcatcaggc gaaccgtatc agcaaccagc gatccgtcgt cctggccctg cccctggcac
     3421 atcacgttgg ccccgacctt cagcgcttcg tccggcgcaa tcaccagctt ggtgaggttc
     3481 gtctgggcgg aggtggtcat ctccacaatg actccgttgc ccacatcgat gcggaccggg
     3541 tccacgccca caactttgcc cgtggcgttg cccatggcct gtggcatgtt cgctcccggg
     3601 ccaccgccgc gctggccccg gcggcccatg ccaaacccca tgccgaaacc gaacccgacg
     3661 ctcaaatccg ttccggcgtc cgctatggtc gcctgattcg cctgcaggga agaaggaaca
     3721 cccactaccg tgatctcaag gccttcggtg atctcatccg tggtcgccgg ctgttgttcg
     3781 agaaccttgg tgtcctgcgt cagcacgacc tggcgcgtga agccctggcc gcccccgggg
     3841 ccgccgccga agcccccgcc gccacccatc tgcaccgcga tgatgccggc ttccatgtcc
     3901 actgcgctaa tggtcccgaa cgccatgttg gccatcgggc cgcccggtcc accgaagcca
     3961 ccgggcccgc cggggccgcc cggctgccct tgcgcgatcc atgccgatag ccccaccgca
     4021 accaccaaca ccgggatgat ccatctcctc atagccgctc agccccttcc atcggatcca
     4081 cgtgtgctcc gtgccaacga tgccatagac gacaccacac gcctaaggtt cggtccgcgt
     4141 cggaccctcg gtcgccgggg cgggcagtag agtctgctcg gtatcatcct cccggaaccg
     4201 gcggtggtac agcgtccggt acagatcaca ccggtcaatc agctccgagt gccttccgcg
     4261 gtccaccacc cgcccttcgt cgatcaccag gatctgcgac gactccgaga ccgtcgacag
     4321 ccggtgcgcg atcatgaagg cgatacggtc atggagcaga actcgaagcg cgtcacggat
     4381 ccgcttctcg gtcagcgggt ccacggacga cgtcgcctcg tccagaatca gtacagccgg
     4441 cgatgccagg acggcgcgcg cgaacgaaat cagctgccgt tggccattcg aaagccgcag
     4501 gcccctctcc tgcacatccg tgtcgtaccc gcgcggcagg tccgagatga actcgtgggc
     4561 attggcaatc cgagccgcct cgacgacctg ctcgtctgtg gcatccgggc ggccgtaccg
     4621 gatgttgtcc cggatggagc ccgagaacag gtgtgtctcc tgctgcacga tggccatttg
     4681 gcctcgcagc gactccagcg ccacgtcccg aatatcggta gcgttgacac gcacggcccc
     4741 ggccttcggg tcatagtggc gcgccaacag cttcacgatg gtcgacttcc
//
LOCUS       DAOSPL010000124         4735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_18077_length_4735_cov_3.210470, whole genome shotgun sequence.
ACCESSION   DAOSPL010000124 DAOSPL010000000
VERSION     DAOSPL010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18077_length_4735_cov_3.210470"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1590)
                     /locus_tag="PLQ54_03505"
     CDS             complement(121..1590)
                     /locus_tag="PLQ54_03505"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOS92352.1"
                     /translation="MGPPFAVREVLLGRDCRSPEILSPSTASRGYLLRRCASVASLIL
                     IDAVSLCVAGIAAAVSPVTVSHTFSPPHILLAVVMLVALFAIHGLYGLRECRRDRRRR
                     ALAAAWFLGTALLLSGAGGAWAPAGVILMWVVAVALLTVGRELLDYCLKAVFPLDLES
                     RRTVVVGSERALATFAARVASLPHEAHARIVGIVSEEPPGFKWPRPHGLPLLGQMRDI
                     EAIICRWKPDELVVVDHDTERLHLVELAELCRRRRMTLKLSDLDMRFQERGVSLIPGL
                     GEALFVAASSPPNGLAWAFKRWMDVGLAMVLLILTAPLWAAVAAAIKLTSAGPVFHSA
                     ERVGLGQQAFRCLKFRTMRADAEALQADLESLNEADGAIFKIRDDPRVTALGRWLRKL
                     SIDELPQLVNVLRGEMSLIGPRPLPLRDNQLLEAWHRQRHVILPGLTGLWQVNGRSDT
                     SFDEMIRLDLKYIDTWSLRLDLSIAWRTAVAVIRSRGAC"
     gene            complement(1666..2457)
                     /locus_tag="PLQ54_03510"
     CDS             complement(1666..2457)
                     /locus_tag="PLQ54_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOS92353.1"
                     /translation="MIARRVLGMRVDCLEAPAARDMIVAWAAETHDAGYRPRYICAGN
                     VHMTMETYDDPAFRAAVNRSDLVVADGQPLVWALRALRAPQRRRVRVTPDLLLELFAA
                     CEVRGVRLGLYGGTPQTLSVFLDFLQHSFPRLQVPYAWSPPFKPPTDEEDEAAVAAIR
                     AAGVQLLLVGTGCPKQEKWMAAHAGEKDIEDDRALSCVMFGVGAAFDLLGGKTSSAPR
                     WMQDLGLEWAYRLAHEPRRLWRRQLKHNPRFVVLFLAQLLRGQRA"
     gene            2714..4180
                     /locus_tag="PLQ54_03515"
     CDS             2714..4180
                     /locus_tag="PLQ54_03515"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOS92354.1"
                     /translation="MGRRVAAPGIRGAGPVLTAALIVAPVAAAGWLLIFGRTGNAVAV
                     LLCTLPVGLSVLSLSAARPLMLSLVVITPFWAIAPAARPHDLNWYAYLALAGGLLVVR
                     DGVELRRAARDPVVLLIVYLLCSFAVLFIVGVPVAIGDCVMLANGLWLYWLLRRSSEH
                     ERSLFISLVLAFAVAQASIGICQSVFGFPTFRTVIPELAIEERNPLAFFFAGLSNDGA
                     SASGTFSHFNALGALLALALPLLMYRIPRRLFRVCRPAALALIALGLWFTYSRGAWIG
                     AALGVGAALWWLVRRGLDNRQLRRQRRRRRPWLWITVGITVGIALIATAGSLYEPGRA
                     YYLETNNVSSRVTTYRFVWDQTVSSPARVAFGAGFSRFHGDLLPQHRDEHPGILGSVH
                     SVPLQILAEGGVLALTLLVLAVASPVAAPFRRGATREQAAVGGALLAILVAGLVDTAL
                     FGYTGVLAFGLLAVQRGALAAQQPCLSPPSATLHQQAR"
     gene            4255..4728
                     /locus_tag="PLQ54_03520"
     CDS             4255..4728
                     /locus_tag="PLQ54_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92355.1"
                     /translation="MPESKTSVLAIRNERPLGSHSDVYEALDRLAADGVMDEYVVYPY
                     LQRLHEGVSHSAISEGILETARQRCADLIIWMHTGSLMVSDECLESLRALPDSPTMVY
                     WEGDSYHSWFKPLPSSMLTVMRRCETVYLPCGGPIVRVLKRAGCRDIRYAPSCTS"
BASE COUNT          776 a         1518 c         1569 g          872 t
ORIGIN      
        1 aaagccggcc cctcccgtca ctaccgacgt cgtcacgctg actctccttg gttcgatcgg
       61 gaatgagggt tccctggtct ttgcggatta cggtcgcgcc gcgatgggcg catccgtttg
      121 ttagcaggcg cctcgggagc ggataacggc cactgccgtg cgccaagcga tggacagatc
      181 gagtcgtaag gaccatgtgt ctatgtactt caggtcgagg cggatcatct catcgaaaga
      241 ggtgtcgctg cggccattta cctgccacag ccccgtcaat cccggcagga tcacgtgacg
      301 ctgccgatgc catgcctcaa gcaactggtt gtcccgcagc ggcagcggcc gcgggccgat
      361 caggctcatc tcgccgcgga gcacgttgac gagctgagga agctcatcga tgctcagttt
      421 gcgaagccag cgaccgagcg ctgtgacgcg tgggtcatct ctgatcttga agatggctcc
      481 gtcggcctcg ttgagcgact cgagatcggc ttgtagggcc tccgcatccg ctcgcattgt
      541 cctgaacttg aggcaacgga acgcctgctg gccgagtccc acgcgctcgg ccgaatggaa
      601 cacggggcct gcagacgtga gcttgatagc tgcagcgacg gcagcccaga gaggcgcggt
      661 caggatgaga agcaccatcg ccagaccgac atccatccag cgtttgaacg cccatgcgag
      721 accgttcggc ggtgaggagg cggcgacgaa caacgcctcg ccgagtcccg ggatgagact
      781 gacgcctcgc tcttggaacc gcatgtcaag gtcactcagc ttgagggtca tccggcggcg
      841 ccgacacagc tcggcaagct cgaccagatg aaggcgttcg gtgtcgtgat cgacgactac
      901 gagctcgtcc ggtttccagc gacagatgat cgcctcgatg tcccgcatct gccccagcag
      961 cggtaggccg tgaggtctcg gccacttaaa gccgggcggc tcttcgctga cgatcccgac
     1021 gatcctcgcg tgcgcctcat gcgggagtga ggctacgcga gcggcgaagg ttgcgagtgc
     1081 gcgctccgag ccaaccacga cggtgcgcct gctctcgagg tcgaggggaa acaccgcctt
     1141 gaggcaatag tcgaggagtt cccggccgac ggtgagaagg gcgacggcca caacccacat
     1201 caagatcaca cccgccggtg cccaagctcc gccggctccg ctcagcagca gggccgtgcc
     1261 gaggaaccac gccgccgcga gtgctcggcg ccggcggtct cgccggcatt cgcgcagacc
     1321 gtacaatccg tggatcgcga agagggcgac gagcatgacg acggccagca agatgtgtgg
     1381 cggtgagaag gtgtgcgaca cggtgaccgg cgacactgcc gcagcgatgc ccgccacgca
     1441 cagtgagacg gcgtcgatga ggatcaggct cgcaacactc gcgcatcgac gcagcagata
     1501 accccgtgac gccgtggatg gcgacagaat ctccggggat cggcaatcac ggcccaggag
     1561 tacctcgcga acagcgaacg gtggtcccac ctacgccctc ccccctttgt gcccctcgtt
     1621 tcctggcgag actatacggc gctcgtcgga cggcgtaaag agcgcttatg cacgctgccc
     1681 ccggagcaac tgggcaagga agaggacgac gaaccgggga ttgtgtttga gctgtcttcg
     1741 ccagagccgt cgcggctcgt gagcgagccg gtaggcccac tctagaccga ggtcctgcat
     1801 ccagcgtggg gcactggacg tcttgccgcc cagtaggtcg aaggccgctc caactccgaa
     1861 catcacgcaa gacagggcac ggtcgtcctc gatgtccttc tcaccagcat gggcggccat
     1921 ccatttctcc tgcttggggc agcctgtccc caccagcagc agctgcactc ccgcagcgcg
     1981 aatcgccgcc acagctgctt catcctcctc gtccgtcggc ggtttgaagg gagggctcca
     2041 agcgtagggc acctgcagtc tcgggaacga gtgttgcagg aagtcgagaa agacggagag
     2101 agtctgaggc gtgcctccgt acaatccgag tcgcacgccg cggacctcac aggcggcgaa
     2161 gagctcaagc agcagatccg gcgtcacccg cacccgccgg cgctgaggcg cacggagggc
     2221 ccgcagggcc cacaccaagg gttgtccgtc cgcgacaacc aagtcggagc ggttcaccgc
     2281 tgcccgaaaa gcgggatcgt cataggtctc catcgtcatg tggacgttgc cggcgcagat
     2341 gtaacgcgga cggtacccgg cgtcgtgcgt ctcggccgcc cacgcgacga tcatgtcccg
     2401 ggcagcgggc gcctcgaggc aatcgacgcg catgcccaag actctgcggg caatcatgcc
     2461 gacatcccga cgactgccgg tcggggagcc tggcgttccg gcttatcgga ttgtgttgca
     2521 gcgcatggca tatatcgtca ttatctcacc gatcgccggt gtccggtgcg gccccgtccc
     2581 cgatgagccg ggcctggggg cttcgctgag ccaggcgctc cggtcgtgcc cgcgatagga
     2641 ttgttgctgg gcgaagacag ggggctgacg cacattggag cggacgtcgg cgacagaggc
     2701 cggggcggag agcatgggga gacgtgtggc cgctccgggc atccgtggag ccggcccggt
     2761 cctgaccgca gctctgatcg tcgcccccgt ggcagcggcc ggatggctgc tcatcttcgg
     2821 tcggaccggc aacgcggtcg cggttctgtt gtgtacgctg ccggtcgggc tgagtgtgct
     2881 ctcgctctcc gccgcccgcc cgctgatgct atctctcgtg gtcatcacgc cgttctgggc
     2941 catcgcgcct gcggctcggc ctcatgacct gaactggtat gcgtatcttg cgctggccgg
     3001 cggactgttg gtcgtcagag acggcgtcga gttgcggcgc gcagcgcgcg accccgttgt
     3061 tctgctgatc gtctatcttc tctgttcgtt cgcggtcctc ttcatcgttg gggtgcctgt
     3121 tgcgataggc gactgcgtga tgctggcgaa cgggctctgg ctgtattggc tgctgcgccg
     3181 ctcatcggag cacgagcgct ccctgttcat cagtctggtg ctggcgttcg ccgtcgctca
     3241 agcgagcatc ggcatttgtc agtccgtgtt cggcttcccg acgttccgga cggtcattcc
     3301 cgagctggcg attgaagaac gcaatccctt ggccttcttc ttcgccggcc tgtccaacga
     3361 cggtgcgtct gcatcgggga cgttcagtca cttcaatgcg ctgggcgcat tgctggcctt
     3421 ggcgctgcct ctcctcatgt accgaatccc ccggaggcta ttcagggtct gcaggccggc
     3481 ggcgcttgcg ctgattgccc tggggctgtg gttcacgtat tcgcgaggcg cctggatcgg
     3541 ggccgctctc ggggtgggag cggccctctg gtggctggtg cgacgaggtc tggacaaccg
     3601 gcagctgcgc cggcaacggc gccgaagacg tccgtggctc tggattacgg tcgggatcac
     3661 agtcgggata gcgttgatcg cgacggcagg ctcgctgtac gagccggggc gggcgtacta
     3721 cctggagacg aacaacgtct cctccagggt caccacctat cggttcgtgt gggaccaaac
     3781 ggtcagctcg cctgcgcgag tcgccttcgg ggcgggcttc tcgcgcttcc acggcgacct
     3841 gctgcctcaa caccgcgatg agcatccggg gattctggga tcggtgcaca gcgtgccctt
     3901 gcagatcctg gcggagggag gcgtcctcgc gttgaccctg ctcgtgctgg ccgtcgcctc
     3961 accggtcgcg gccccgttca ggcggggcgc cacccgcgaa caggcagccg tgggcggagc
     4021 tcttcttgcc atcttggtcg ccgggctggt ggacaccgca ctcttcgggt atacgggtgt
     4081 gctggcattc gggctcttgg ctgtccagcg cggcgctctc gccgcccagc agccgtgtct
     4141 cagccctccc tccgccacct tgcatcagca ggcacgatga tggcgaatga tcgccgaccg
     4201 ggatgccgcg tatggcgctc cgacggtatt ggcggcgccg acgttggagg cccgatgcct
     4261 gagtcgaaaa cgagcgtgct cgcaatccgg aacgaacggc cgctcggctc gcactcggat
     4321 gtctacgaag cgctggaccg gttggccgcc gacggggtta tggacgaata cgtggtctat
     4381 ccgtatctgc aacgccttca tgagggtgtg tcgcactccg ccataagtga ggggattctt
     4441 gagaccgccc gacagcgctg cgccgacttg atcatatgga tgcacaccgg ctctctcatg
     4501 gtctcggatg agtgcctgga gagcttgagg gctctccccg acagcccgac catggtgtac
     4561 tgggagggcg actcgtatca ttcttggttc aagcccttgc cctcgtcgat gctgacagtc
     4621 atgcgccgct gcgagacggt ctacttgccc tgcgggggcc ccatcgtgcg ggtgctgaag
     4681 agggccgggt gccgagacat caggtacgcg ccgagctgca cttcgtaagc tcgtt
//
LOCUS       DAOSPL010000125         4731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_18095_length_4731_cov_3.167451, whole genome shotgun sequence.
ACCESSION   DAOSPL010000125 DAOSPL010000000
VERSION     DAOSPL010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18095_length_4731_cov_3.167451"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..696
                     /gene="rpsE"
                     /locus_tag="PLQ54_03525"
     CDS             178..696
                     /gene="rpsE"
                     /locus_tag="PLQ54_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOS92356.1"
                     /translation="MSRKARFEDYEADEFEERVIHIDRVKKTVKGGQIISFRALVAVG
                     NKKGVVGFAVGKARGTPDAIRKGVERAKKSLVQVPLSGTTLPHEVTARVGGATVFMRP
                     ASRGTGVIAGGPVRPIVELAGVHDVLAKCLGSESRLNNAAAAFDAIRAVRNPEEVAEL
                     RGKTTDEVLAWS"
     gene            860..1285
                     /locus_tag="PLQ54_03530"
     CDS             860..1285
                     /locus_tag="PLQ54_03530"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOS92357.1"
                     /translation="MPDTAALECLRALIAGAPVSLLVTNRADGPPAIRPMQTVLVDAD
                     FAVWFATGRASDKCRQIAADPRVTVYWQQPDVWHYGEISGVAEVVDTPEARHRFWQDD
                     WSQYFPGGRDDPQYVLIRVTPERAICHHMQDGSTRNVEF"
     gene            complement(1330..2556)
                     /locus_tag="PLQ54_03535"
     CDS             complement(1330..2556)
                     /locus_tag="PLQ54_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92358.1"
                     /translation="MSRNQRLLVAAALACLVTAVAANGYEFSRAAGWSIDWPVSVTAV
                     PGFYGPNTPGTRLQLTWSDSVWGAVSDYIQSDDHSIPDGDVGEVFDVEGLFAKQQGRK
                     LHILVVLSTPESIVDVSTYDPAYRRGTYVMAGDLALNRGAAAAPEDGPARSYAYTAEL
                     GIKTAGPHAGHAERYARWGYGSQGPTADPSADWRDPAVDQGENPKGYAPGEQVGEFTG
                     YPWASNFWHGTGSEFSGIVRVSDWRLVLTGSDDPSDAAPANNTYAYEVVIDTGDVAHA
                     ITDIMVAASCLNDGLALTLDEGGSPGGGGGGGGALAALPLPALGGLLPWEGAAAAPVM
                     AGGVVDEVLDESDEQVLPEENIPDDEPPPNPPRGGGDDDPVATPEPVVLSLLFAAAGA
                     GLAANRTRRGRGARRG"
     gene            complement(2794..3354)
                     /locus_tag="PLQ54_03540"
     CDS             complement(2794..3354)
                     /locus_tag="PLQ54_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOS92359.1"
                     /translation="MHSVLIVAGLAFGLAMDAFAVAIAVGAALGRPTSRQFFRLAFHF
                     GLFQFMMPVIGWFAAEGASAVLARVDHWVALGLLSFIGGKMIVEALRGDHEQVRGDPT
                     RGASLVMLSVATSIDALAVGLSLKAAGVTLWFPCVAIGVCAASMTFMGMKLGRRGASR
                     LGQRAEVAGGIVLILIGIRIAIDHLI"
     gene            complement(3355..>4731)
                     /locus_tag="PLQ54_03545"
     CDS             complement(3355..>4731)
                     /locus_tag="PLQ54_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS92360.1"
                     /translation="HARLAAVVCLFLPLFVLGLLRFGRRIRRAFLAVREKVGVLTGTV
                     AEQIAGVRVIRAFVQEARMAARFRAVNQDNADANITATRVMAQFVPLVEAIAVLALVA
                     VMWCSGRLLAADGTASNAAEAVTLGTVTAFIGYLNRLFVPVRNLSRLYSTVQSAFAGA
                     ERVFEILDERPSVAEALDAQPLAPLSSEVRFESVSFAYPSAGDEASRGTQAPRQALSD
                     VSFALAVGQRTALVGPTGAGKSTVVKLLSRFYDPQQGRVLFDGVDVRRATFRSLRGQM
                     AVVLQETYLFSGTVAENIRYGRPEATDEETEAAARAVSAHEFILRLPQGYATPVGGDG
                     VALSTGLRQLVAFARAILCNPRLLILDEATSSVDVCTERVIRHALRQLLAGRTALIIA
                     HRLSTVMDSDRILVLDGGRLVGEGTHEELLATCPLYGRLYALQFRDADVRLAAGEGHP
                     SRRPRRSG"
BASE COUNT          809 a         1632 c         1543 g          747 t
ORIGIN      
        1 aggggccccg cgccctctgg gcgcacccct gtgcggcccc tccgcaagac caggcagggg
       61 gctgtgtgag gctgttttga caccgatccc gcctcgtggt agcatatggg tttgtgtgac
      121 atgaatgggt gcgggcgccg cgcccgcttt agcttcggtt tggagaaacc ccaacacatg
      181 agcaggaaag ccaggttcga ggactacgag gccgacgagt tcgaggagcg cgtcatccac
      241 atcgaccgtg tgaagaagac ggtcaaggga ggacagatca tcagcttccg ggcgctcgtc
      301 gccgtgggca ataagaaggg cgtcgtaggg ttcgccgtgg gcaaggctcg cggcacgcca
      361 gacgcgatcc gcaagggcgt cgagcgggcc aagaagagcc tcgtccaggt gccacttagc
      421 gggacaaccc tgccccatga ggtcaccgcg cgtgtcggcg gcgcgacggt cttcatgcgc
      481 ccggcttctc ggggtaccgg tgtcattgcc gggggtcccg tgcgtcccat cgtcgagctg
      541 gccggcgttc acgacgtcct cgccaagtgc ctgggcagtg agagccgcct gaacaatgcc
      601 gcagcggcct ttgatgccat cagggccgtc cgcaatcccg aagaggtcgc cgagcttcgg
      661 ggcaagacca ccgatgaggt cctggcctgg agctgaccgg ccgtcgggcc tgcgcttgtc
      721 ctctaggggc tgtcgccatg ggcgacggcc tctttcgtgc gtctgccggg ccatcgaagg
      781 cagacggacc gtgagtggcg aacagctcct tgcccctacc gcgcgggtga ggctgtttac
      841 agcctgagga ggatggacca tgcctgacac cgccgctctc gagtgccttc gtgccctcat
      901 cgccggtgct ccggtctcgc tcctggtgac caaccgcgcg gatggcccgc cggccatccg
      961 ccccatgcag accgtccttg tcgatgcgga cttcgccgtg tggttcgcca ccggccgtgc
     1021 ctccgacaag tgcaggcaga tcgccgccga tcccagggtc acggtctact ggcaacagcc
     1081 agacgtgtgg cactacggtg agatcagcgg tgtggcggaa gtggttgaca cgcccgaggc
     1141 gcgtcaccgc ttctggcagg atgactggtc gcagtacttc ccggggggta gggacgatcc
     1201 gcagtatgtc ctcatccgcg tcactccgga gcgcgccatc tgccaccata tgcaggacgg
     1261 gagcacacga aacgtggagt tctgagggcg tgtcctcgcc gatcgccggc ggggacgggt
     1321 cgcatgcgct cagcctcgcc tggctccgcg cccccggcga gtccggttag ccgccagtcc
     1381 ggcaccggct gcagcgaaca gcaacgagag gaccacgggc tcgggtgtcg ccacgggatc
     1441 gtcgtcgcct cccccacgtg gagggttcgg cgggggctca tcgtcaggga tgttctcctc
     1501 gggtagcacc tgctcgtcgc tctcgtcgag cacttcatcc acgacgcccc cggccatgac
     1561 gggggctgcc gcggcgccct cccacgggag tagcccgccc agcgcgggca ggggcagggc
     1621 agctagcgcg ccgccgccgc cgcctcctcc ccccggactc cccccctcgt cgagggtgag
     1681 cgccaggccg tcgttcaggc aactcgcggc caccataatg tcagtgatgg cgtgagcgac
     1741 gtcgccggtg tcgatcacga cttcgtaggc gtaggtgttg ttcgccggcg cggcgtctga
     1801 tgggtcgtcg gagccggtca gcaccagacg ccagtccgac accctcacga ttccgctgaa
     1861 ctcgctcccg gtaccgtgcc agaagttcga ggcccaaggg tagccggtga actcacccac
     1921 ttgctctccc ggagcatacc cttttgggtt ctccccctga tcaaccgccg ggtcacgcca
     1981 gtcggccgat gggtcagccg tggggccctg ggaaccgtag ccccaccggg catagcgctc
     2041 ggcgtggccc gcatggggac cggcggtctt gatgccgagc tcagccgtgt acgcgtatga
     2101 cctggccggg ccatcctcgg gcgcggcagc agctccacgg ttcagcgcga ggtcgccagc
     2161 catcacgtaa gttcctcgcc gataggcggg gtcatatgtg ctgacatcca caatgctctc
     2221 gggggtgctc aggacgacga ggatgtgaag cttgcggccc tgctgcttgg cgaacagccc
     2281 ctcaacgtca aagacctcgc ccacgtcacc atcggggatg gagtggtcgt ccgattggat
     2341 gtagtcactc acggcgcccc acacagagtc cgaccacgtc aactggagac gtgtccccgg
     2401 tgtgttcggg ccgtagaaac ccggcacagc cgtgaccgac acgggccagt cgatggacca
     2461 gcccgccgcc cgactgaact catatccgtt cgcggccacg gcggtgacga gacacgccag
     2521 agcggcagcc accagcagcc tctggttcct gctcatcgtg atccactctc ccacgggtac
     2581 cgcccccacc ccaacccact ccaacccagg cagcaaatgc cgcccgggcc actcctccat
     2641 ctcaggtatt ccccatctct ggcccagttc atcgcccgtg agctggattt ggggcgggtt
     2701 tttcagggtt ctacctgaca atcgcgccca ccttcgtgcg tctgtgggcg cggttctggc
     2761 ggtgcctgcg gcgagactga ggggcggaat cggttagatc aggtgatcga tggcgatacg
     2821 gattccaatg agtatcaaga cgataccacc ggcgacctcg gcacgctgac ccaggcgcga
     2881 cgcaccgcgg cgccccagct tcatgcccat gaaggtcatc gaagccgcac agaccccaat
     2941 cgcgacacac gggaaccaga gggtcacccc cgccgccttc aagctcaggc ccaccgccaa
     3001 ggcgtcgatg ctcgtggcca cggagagcat gaccaaggac gcgcctcgcg tcggatcacc
     3061 cctgacctgc tcgtgatcgc cgcggagcgc ctccacgatc atcttgccgc cgatgaacga
     3121 gagcaacccg agcgcgaccc agtggtcgac gcgggccagg acggctgagg cgccctcggc
     3181 ggcgaaccag ccgatcaccg gcatcatgaa ctggaacaga ccgaagtgaa aggctagtcg
     3241 aaagaactgg cggcttgtgg gtctcccaag ggcagcgcct acggcaatag ccaccgcgaa
     3301 ggcgtccatc gcgagcccga aggccaagcc cgccacgatc agcacgctgt gcaactatcc
     3361 gctccttcgg ggcctccgcg acggatggcc ttcaccggcg gcaagccgta cgtcggcgtc
     3421 gcggaactgc agagcgtaca gcctgccgta cagggggcaa gtggccagga gctcctcgtg
     3481 agtgccctcg cccaccagac gcccgccgtc gaggaccagt atacggtccg agtccatcac
     3541 tgtggacagc cggtgcgcga tgatcagcgc cgtgcggccc gccagaagct gcctcaacgc
     3601 gtgccggatg actcgctcgg tgcagacgtc gacgctcgat gtcgcctcgt ccaggatgag
     3661 cagtcgcggg ttgcacagta tggcacgcgc gaaggcaacg agttggcgta gcccggtcga
     3721 gagagccaca ccgtcgcccc ccacgggggt ggcgtagccc tgcggcagac gcaggatgaa
     3781 ctcgtgggcc gagacggcgc gcgccgcggc ctcggtctcc tcgtcagtgg cctccggccg
     3841 cccgtagcgg atgttctcgg ccacggtccc gctgaagagg tatgtctcct gcagcaccac
     3901 cgccatctgc ccgcggaggc tgcggaatgt ggcacgacgc acgtccacac catcgaacag
     3961 cacgcggcct tgctgcgggt cgtagaagcg cgagaggagt ttgaccactg tgctcttgcc
     4021 ggcacccgtg ggccccacca gggctgtgcg ctgcccgacc gccagggcga aactcacatc
     4081 cgagagcgcc tgccgggggg cttgtgtccc gcgggaggcc tcgtcgcccg cactcggata
     4141 ggcgaagctc acgctctcga accgcacctc gctggagagc ggtgccagag gctgagcatc
     4201 cagcgcttcg gccaccgacg gccgctcgtc gaggatctcg aacaccctct cggctcccgc
     4261 gaaagccgac tggaccgtcg agtagagtcg cgagaggttt ctcaccggga cgaacagacg
     4321 gttcagatag ccgatgaagg ccgtcaccgt ccctagcgtg acggcttcgg cggcattgga
     4381 ggccgttcca tccgcggcca gcagtcggcc ggagcaccac atcacggcga ccagtgctaa
     4441 caccgcgatg gcttccacca acggcacgaa ctgcgccatg acccgggtcg cggtgatgtt
     4501 cgcgtcggca ttgtcctggt tcacggcgcg gaaccgggcc gccatgcgcg cctcttgcac
     4561 gaacgcgcgt atcaccctca ccccggcgat ctgctcggcc acggttccgg tgagcactcc
     4621 caccttctcg cgcacggcca ggaaggcacg gcgaatgcgg cgcccgaagc gcagtagacc
     4681 caggacgaac agtggcagga acaggcacac cacggctgcg agcctggcgt g
//
LOCUS       DAOSPL010000126         4730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_18103_length_4730_cov_3.405989, whole genome shotgun sequence.
ACCESSION   DAOSPL010000126 DAOSPL010000000
VERSION     DAOSPL010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18103_length_4730_cov_3.405989"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PLQ54_03550"
     CDS             complement(<1..370)
                     /locus_tag="PLQ54_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92361.1"
                     /translation="MIWAVIRKELQEIRRTKLVLYTMAVTPTLFLLTLWFTLGPLCGE
                     VAKARDAAEAGSVTGATQAQEGADGAAAAGQTEQQARMAAEAAKGRDMLSRMLTYWMV
                     LMMTVPMMVPSVILAYSIIGE"
     gene            complement(367..1290)
                     /locus_tag="PLQ54_03555"
     CDS             complement(367..1290)
                     /locus_tag="PLQ54_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS92362.1"
                     /translation="MVQATGLTRRFGQHTAVDGVTLHVDSGTVFGLLGPNGAGKTTTV
                     RMLVGLIAPTSGDATVAGHRVGPGNDQIRRHVGVLTESPGLYSKLTVRQNLEYHGRLH
                     GLRGPTLSRRIKDYLNLVEMSASVNTLCGALSKGMKQKVAIARALVHEPSVLFLDEPT
                     SGLDPESAALMRDFLVTLKADHRTVFLCTHNLAEAERICDVVGVLKQRLIAVDTPANL
                     SRGSAPRVRIRLAGDDPAAYLDAVCGLTGVDGVSVDGGVLVANVADPAAQNPAIVAAL
                     VRAGAQVVFVEEERHSLEEAYLHLVREEATQ"
     gene            1443..2537
                     /locus_tag="PLQ54_03560"
     CDS             1443..2537
                     /locus_tag="PLQ54_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92363.1"
                     /translation="MSTNDASAGGASLEFKPDLAEATRHWLAFWAQDIIDRPCCCIRS
                     PRAGGSVPPGPPYLAGAQDDFGPVIEQILDHFGATYFAGDAIPAYTPSFGPDQMAAWV
                     GAELDFSADGFGTSWVKPLVEDWEAALPLAIDTASHWWRRMLAFLRALSEALRGKMII
                     SHLDLHSNMDLLSALRLPARLCMDMVDCPETVDRAMASARALYAPIYEALYEAGAMHE
                     HGTTGWVPAYHPVRTNTIQCDFAALIGPAHFRRWVLPALEEEAAYLGHCVFHLDGPEC
                     LVHLNDLCAVPGIDCIQWVHGARNKPFIEWMDLLKDIQAKGTSVWVPCGVDDIRVFHR
                     ELQPNLVFYDCWAPTQKQADETLAWLISTT"
     gene            2690..3580
                     /locus_tag="PLQ54_03565"
     CDS             2690..3580
                     /locus_tag="PLQ54_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92364.1"
                     /translation="MRLLIATLFTTAGPVLLMGCSRSDAQNTTAAAPAPPVTTAAVAA
                     AAPGPERDGEAIVIDHNCLDVAAIPDEWIEAAKSKLRIHYAHTSHGGQIMSGLAALAQ
                     TDPKFAYKAANSSLPTGAGALCIFDGQESESYITPELYWATPEGLAATQAVLDHNPSI
                     NVSLWSWCCQQDHNTAEQTQAYLDAMAGLEAKNPKVTFVYMTGNAQGRDGGGLNRHLR
                     NEQMREYCRANGKVLYDFADIECWYGGDECTAMFEGKSYPHEHTQFGKDEDSHTSKEN
                     CLNKGKAFWWLAARLAGWKG"
     gene            complement(3595..4338)
                     /locus_tag="PLQ54_03570"
     CDS             complement(3595..4338)
                     /locus_tag="PLQ54_03570"
                     /inference="COORDINATES: protein motif:HMM:NF021449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2161 family putative PD-(D/E)XK-type
                     phosphodiesterase"
                     /protein_id="HOS92365.1"
                     /translation="MSAPTASTLAETDLYAPVRDYLADQGYVVQGEVKKCDIAATRDG
                     KLVVVEMKLRLSAEALAQAAQRQEIADEVYLAIPRPKALTAWRRRSGHWLYLIRRMEL
                     GLLLVSPRARKASRVAVEQPVRIWDSVKQGGRRGAVVREVQRRAGDFNEGGSVRRRLL
                     NSYRQTAIHIACCLERFGPLTIRQLHRLGTGLETRSALENSLRIEKWFRLKPPSTYAL
                     TDEGRAGLVEFAHAAEHYRLALRDREPEP"
     gene            complement(4349..>4730)
                     /locus_tag="PLQ54_03575"
     CDS             complement(4349..>4730)
                     /locus_tag="PLQ54_03575"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOS92366.1"
                     /translation="DEMCSGSERLYVPIIPQEWNLPEMLLAVAETALLPTTCPCFSRH
                     DDRLTRIGELVDAYSADGVVYHNLRLCALYQFEATAVRDALAARSVPMLELQTDYTPE
                     DTEQMHTRVQAFLEMLEARAPVPG"
BASE COUNT          815 a         1574 c         1562 g          779 t
ORIGIN      
        1 tctcgccaat gatgctgtag gccaggatga ccgaaggcac catcatgggg accgtcatca
       61 tgaggaccat ccagtacgtg agcatccgac tgagcatgtc gcggcccttg gctgcctcgg
      121 ccgccatgcg ggcctgttgc tcggtctgcc cagcagcggc ggcgccatcg gcgccctcct
      181 gggcctgcgt cgctcccgtc acactgcccg cctcggcggc atcacgcgcc ttggcgactt
      241 ccccgcacag aggcccgagc gtgaaccaga gcgtgaggag gaacagggtg ggcgtgacag
      301 ccatggtgta tagcaccagt ttggtgcggc ggatctcctg tagctctttg cggatcacgg
      361 cccagatcat tgcgtcgcct cctctcgcac caggtgcaga taggcctctt cgagcgagtg
      421 ccgctcttcc tcgacgaaga ccacctgcgc tcccgcccgc accagcgccg caacgatggc
      481 agggttctgc gccgccggat cggccacgtt ggcgacaagt acgcccccgt cgaccgagac
      541 gccatccacg cccgtcagtc cacacacggc atcgagatag gcggccgggt catcaccggc
      601 caggcggatg cggacccgcg gggcagaccc gcgcgatagg ttggcaggcg tgtcgacggc
      661 gatgagccgc tgcttgagca cgcccacgac gtcgcagatg cgctcggcct cggccaggtt
      721 gtgggtgcac aggaacaccg tgcgatggtc ggccttgagt gtcaccagga agtccctcat
      781 gagagcagcc gactcgggat cgaggccgct cgtgggctcg tccaggaaca gcacgctcgg
      841 ctcgtgcacc agcgcccggg cgatggccac cttctgcttc atgcccttcg agagggcgcc
      901 gcacagggtg ttcacgctgg ccgacatctc gaccagattg aggtagtcct tgatgcgacg
      961 gctcagggtc gggccgcgca gaccgtgaag gcgcccgtgg tactcgaggt tctgccgcac
     1021 ggtcagcttg ctgtagagtc cgggcgactc ggtaagcacg ccgacgtgcc ggcggatctg
     1081 gtcattgccc gggccgacac gatggcccgc aaccgtggca tcaccgctcg tcggcgcgat
     1141 gaggccgacc agcatccgaa ccgtggtggt ctttcccgca ccattcgggc cgagcagccc
     1201 gaaaaccgtg cccgaatcca cgtgaagggt aacgccgtcg actgccgtgt gttggccgaa
     1261 cctccgggtg agacccgttg cctgaaccat cgcaccactg ctcctcctgg gcgtcacttc
     1321 ctctgcagac gatgacacaa ggcacaagtc gcgcgcaact tcggccgagg tggccgcggc
     1381 ggcgtggcgg aagacgacgg cgcccgactt ggggcactga ctacgagagc ggcgggatac
     1441 ttatgtcgac gaacgacgcg agcgccggtg gcgcctccct ggagttcaag cctgacctgg
     1501 cggaagccac gcgacactgg ctcgccttct gggcgcagga catcatcgac cggccctgct
     1561 gctgcatccg gtcgccgaga gccgggggct ccgtacctcc gggcccaccg tacctcgccg
     1621 gagcccagga tgacttcgga cccgtcatcg agcagattct cgaccacttt ggcgccacgt
     1681 acttcgccgg ggatgcgatt ccggcctaca ccccgagctt cggcccggac cagatggccg
     1741 cgtgggtggg tgccgagctc gacttctccg ccgacgggtt tgggacgagc tgggtgaagc
     1801 cgttggtcga ggattgggag gcagccctgc ccctggccat cgataccgcg agccactggt
     1861 ggcggcgcat gcttgccttt cttcgggcgc tgtccgaggc gctgcgcggc aagatgatca
     1921 tctcgcacct cgacctgcac agcaacatgg acctgctgag cgcactacgc cttccggcaa
     1981 gactgtgtat ggacatggtg gattgcccgg agaccgtcga ccgcgcgatg gcgagcgcac
     2041 gagccctata cgccccgatc tacgaagccc tctacgaggc aggagccatg cacgagcacg
     2101 gcaccacggg gtgggtgccg gcctatcacc cggttcggac gaacaccatc cagtgcgact
     2161 tcgccgcact catcgggccg gcgcacttcc gccgatgggt attgccggca ctcgaggaag
     2221 aggcggccta cctgggccat tgcgtgttcc atctcgacgg ccccgagtgt ctggtgcacc
     2281 tcaacgacct gtgcgctgtg cccggcatcg actgcatcca gtgggtccac ggcgcgcgga
     2341 acaagccctt catcgagtgg atggacctgc tgaaggacat ccaggcgaag gggaccagtg
     2401 tgtgggtgcc atgcggggtc gatgacatcc gcgtcttcca ccgggagctt caaccgaacc
     2461 tggtcttcta cgactgctgg gcaccgacac agaagcaggc ggacgagaca ctggcttggc
     2521 ttatatcgac cacctaataa cactactgct tcgggtccgt gctcgtcagc agcgcaagcc
     2581 aattcggggt ttggaaaccg gcggaacgag gtatatgtaa ttggcacttt gtgccggtcc
     2641 gcctttgggc gggcggtcca gtgttgaccc acctagggag agtacccctg tgagactgct
     2701 catcgccacg ctgttcacga ccgcagggcc cgttctgctc atgggctgct cgcgcagtga
     2761 cgcacagaac accaccgcag ccgcgccggc cccgccggtc acgaccgctg cggtggccgc
     2821 cgccgcgccg gggccggaac gcgatggcga ggcgatcgtg atcgaccaca actgcctcga
     2881 cgtggccgcg attcccgatg agtggatcga ggcagccaag tcgaagctgc gcatccacta
     2941 cgcccacacg tcgcatggtg ggcagatcat gtccggcctg gccgctctgg ctcagactga
     3001 cccgaagttc gcttacaagg cggcgaacag ctccctcccc accggtgctg gtgcgctgtg
     3061 catcttcgat gggcaggagt cggagagcta catcaccccc gagctctact gggcgacccc
     3121 cgagggcctg gcggccactc aggccgtgct ggaccacaac ccgtccatca acgtgtccct
     3181 gtggtcctgg tgctgccagc aggaccacaa cacggcggag cagactcagg cgtacctcga
     3241 tgccatggcc ggccttgagg cgaagaaccc caaggtgacc ttcgtctaca tgaccggcaa
     3301 cgcccagggg cgcgacgggg gcgggctcaa ccggcacctg cggaacgagc aaatgcgcga
     3361 gtactgccgg gcgaacggca aggtcctcta tgacttcgcc gacatcgagt gctggtacgg
     3421 gggagatgag tgtacggcga tgtttgaggg caagagctac ccccatgagc acacgcagtt
     3481 cggcaaggat gaagatagcc acacgtccaa ggagaactgt ctgaataagg gcaaagcctt
     3541 ctggtggctt gcggctcgcc tggcggggtg gaaagggtag gacacccgcg tccgctacgg
     3601 ttcgggctcg cggtctcgca gggccaggcg gtaatgctcc gccgcgtggg cgaactcgac
     3661 cagccccgcg cgcccctcat cggtgagagc gtacgtggag ggcggcttca gccggaacca
     3721 cttctcgatg cgcaagctgt tctcgagagc gcttcttgtc tcgaggcccg tccccaggcg
     3781 gtggagctgg cggatggtca gcgggccgaa gcgctccagg cagcaggcga tatgaatggc
     3841 cgtctggcgg taggagttca ggagcctccg ccggacgctg ccgccctcgt tgaagtcacc
     3901 ggcgcggcgc tggacctcgc gcaccacggc gccgcgccgc cctccttgct tcaccgagtc
     3961 ccagatccgc accggctgct cgacagcgac gcgcgaggcc ttccgggccc gaggtgacac
     4021 cagcagcaag cccagctcca tgcggcgaat gagatacagc caatgcccag agcggcggcg
     4081 ccaagccgtc agtgccttcg ggcgcgggat ggcgaggtag acctcgtcgg cgatctcctg
     4141 ccgctgcgcc gcctgggcca gtgcctcggc gctgagccgg agcttcatct cgaccaccac
     4201 aagcttccca tctctggtgg cggcgatgtc gcacttcttg acctcgccct gcacgacgta
     4261 gccttggtcg gccaggtagt cgcggaccgg ggcgtagagg tcggtctcgg caagggtgga
     4321 tgcggtggga gcggacatag ccggtagctt agcccggaac aggcgctctg gcctccagca
     4381 tctcgaggaa agcctgcacg cgcgtgtgca tctgctcggt gtcctcgggc gtatagtccg
     4441 tctgcagctc gagcatgggc acggaccggg ccgcaagcgc atcgcgcacg gccgttgcct
     4501 cgaactggta gagggcgcac aaccgcaggt tgtggtacac cacgccgtcg gccgagtagg
     4561 catcgaccag ctcgccgatg cgcgtcaggc ggtcatcgtg tcggctgaag catgggcacg
     4621 tggtgggcag gagcgccgtc tcggccacgg ccaggagcat ctcgggcagg ttccactcct
     4681 gcgggatgat cggcacatag agcctctcac taccggagca catctcatcg
//
LOCUS       DAOSPL010000127         4678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_18363_length_4678_cov_3.020549, whole genome shotgun sequence.
ACCESSION   DAOSPL010000127 DAOSPL010000000
VERSION     DAOSPL010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18363_length_4678_cov_3.020549"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PLQ54_03580"
     CDS             <1..331
                     /locus_tag="PLQ54_03580"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOS92367.1"
                     /translation="NPNMVKGQMCGGLVQGLGECLWEEIRFDGEGRVANIGFGDYRLP
                     TAADVPEIECHIVEGETSTSRGHGCKGVGEPAHVPALAAAANAVCAAIGRRIYESPLS
                     PERILGA"
     gene            377..2980
                     /locus_tag="PLQ54_03585"
     CDS             377..2980
                     /locus_tag="PLQ54_03585"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOS92368.1"
                     /translation="MALGSLIALLAVNATGEVREYLGSPTLYVNGQPAVPLVFFGWAG
                     GPGPQVVQLAPEWRQFHVTFVAPEDADGPAGVHFRMGGGPPGTVWVDDLRVYQGEFRE
                     NPPESLLRQSSFEGTRDEVAAAWSMFQAEYAGADASWELDRTDAVEGKQSLKVTIRNS
                     GTNTMHIHFFQSGLRVRKGDTYTYSLWMKSAETRTVDFMALHIGEPWTIYHSPSAGPY
                     ASQVRRSAAAGVHIQSFGIDMPWPRPGEEPDWRGVDDPIDLTLANDPDALLLPRFGCG
                     PPGWWLEEHPSERMLFSDGQTASWSMASALWRAEFPEHLRALVRHCEEKYGHHMLGYH
                     PCGQHTGEWFYERSWEPVLSDFSEPMSAGFRAWAATKYRTDEALRTAWGDPAATVRHI
                     RIPTAEEQQRTTLGLFRDPVRERKVIDYFEYKQLAMEEPLEQMARVIKDETERQKLVV
                     FFYGYLFDMHGIPMGPQSSGHLAMARMLRCPDVDVLTSPISYLDRELGGAGLFMSAVD
                     SVRAAGKLWLNEDDTRTYLTPEDSGFGRVDTPQGTFWVHQRNFAQLLPRRLACWYMDL
                     GGIGWLDGQDLWDNIARLREVYEDRLPTPATFAPEVAVIVDEVSPFYTQCTSALHSPL
                     VYQMRSQMARLGAPARIHLLSDLVEGRVPRAKVYVFLNCFHLSDTARRAISRTTAGRA
                     AIWFYGGGFLGDDAAPVTAEGEFERRMSDAVGLPLRRTVVQPGIARLVEDDRSIGTDL
                     TLEPCWAVDEGAGAEVIARYADGSIAAAWRQTREGLRAYVGALHCPASVLRPLLRRAG
                     VHLYATSDDVVLADDRFLGLAATSAGAKRIRLRRPATVIDLLSGEVLGANVDGFELTM
                     ALGETRLFGLR"
     gene            3058..4575
                     /locus_tag="PLQ54_03590"
     CDS             3058..4575
                     /locus_tag="PLQ54_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOS92369.1"
                     /translation="MSGAEPRGALNLLVLADLHYIHHARHQCGVPQRRADMGLELVTR
                     AVRRARRIADIDVIVLLGDLVDNGWAEGAERDLAELRDALEKPGLPVIVVPGNHDGPP
                     ERVRAVFGAETGVGQIAGYQFATFADTYGPGDRCVRPVADLVHTLCRARHQADQPIIA
                     LQHNPVHPPIESAYPYMLEERAQVMELYAESGVALSLSGHYHPGQPPTVVRGVTYATC
                     PALCEAPFRFMLVRMEGRQANVETLSLAMDPETGLVDHHCHTHYAYCAQDITAEKVIA
                     RARDMGLARTHLTEHAGQLYMAADDFWKGAFVTDPDLWIRARAERNRMPAFRAEIGPL
                     RRPEVRWGLEVECDGLGRLTLADEDREGWDVLVGALHYLPATVGAGAMDPARLTREFM
                     TFSEHLLRQGAQILAHPFRIIRKAKVPVPEWLFAPLVDMLASYGAAAELNFHTNQPDP
                     RFFAACLERGVRISLGSDSHAMYEVGDFAPHLRLLEDIGVTRDRLPDVLFGLSAS"
BASE COUNT          735 a         1616 c         1581 g          746 t
ORIGIN      
        1 caacccgaac atggtcaaag ggcagatgtg cggcgggctg gtccagggac tgggcgaatg
       61 cctctgggag gagatccggt tcgacggtga gggccgcgtc gccaacatcg gattcggcga
      121 ttaccgcctg ccgaccgccg ctgacgtgcc ggagatcgag tgccacatcg tcgaaggcga
      181 gacgtcgacc agccggggcc acggctgcaa gggggtcggc gagccggccc acgtcccggc
      241 gttggcggcc gccgccaatg cggtgtgtgc cgccatcggc cgtcgcatct atgaaagccc
      301 gctgagcccg gagcgtatcc tcggcgcctg acgagcccgc gggccggtcc ggcatgcctc
      361 ccaaaggagc cgtcccatgg cacttggcag cctcatcgcg cttctggcag tgaacgccac
      421 aggggaggtg cgggagtacc tcggttcgcc gaccctctac gtcaacggcc agcccgccgt
      481 gcccctggtg ttcttcggtt gggcgggagg acccgggccc caagtagtgc agctggcacc
      541 cgaatggcgc cagtttcacg tcacctttgt ggccccggaa gatgccgacg gcccggcggg
      601 cgtgcacttc cgcatgggcg gaggccctcc gggaacggtc tgggtggatg acctccgcgt
      661 ctaccagggc gagttccgtg agaacccacc cgagagcctg ctcagacaaa gcagcttcga
      721 gggcacgcgg gatgaggtgg ctgcggcgtg gagcatgttc caggcggagt acgcgggcgc
      781 ggacgcatcg tgggagctcg atcgcacgga cgctgtcgag ggtaagcaat cgctgaaggt
      841 caccatccgc aactccggca ccaataccat gcacatccac ttcttccagt ccgggctgcg
      901 tgtccggaag ggagacacgt acacctactc gctgtggatg aagtcggccg agacgcgtac
      961 cgtcgacttc atggccctgc acatcggaga gccctggacc atctaccact ctcccagcgc
     1021 cggcccctac gccagccagg tgcgtcggtc ggccgcggcc ggggtgcaca tccagtcctt
     1081 cggcattgac atgccgtggc ccaggccggg cgaggagccc gactggcgcg gcgttgatga
     1141 ccccatcgac ctgacgctgg ccaacgaccc cgacgcgcta ctgctgcctc gcttcggctg
     1201 tggcccgccc ggctggtggc tcgaagagca cccttcagag cggatgctgt tctccgatgg
     1261 ccagacggcg tcgtggtcca tggcttccgc gctctggcga gccgagtttc cagagcatct
     1321 gagggccctc gtgcggcact gtgaggagaa gtacggccac cacatgctcg gataccatcc
     1381 gtgcggtcag cacaccggcg aatggttcta cgagcgctcg tgggagcctg tgctcagcga
     1441 cttttccgaa cccatgtcgg ccggcttccg tgcctgggct gccaccaagt accggactga
     1501 cgaggccctg cgcacggcct ggggcgaccc cgccgccacc gtccgccaca tcaggatccc
     1561 gacggccgag gagcagcagc gcacgacgct cggactgttc cgcgaccccg tgcgcgagcg
     1621 caaggtcatc gactacttcg agtacaagca gctcgccatg gaagagcccc tcgagcagat
     1681 ggcccgggtc atcaaggacg agaccgagcg gcagaagctg gtcgtcttct tctacggcta
     1741 cttgttcgac atgcacggca tccccatggg gccgcaatcg agcgggcacc tggccatggc
     1801 gcggatgctg cgctgccccg acgtcgacgt cctgacctcg cccatctcct acctcgatcg
     1861 cgaactcggc ggggcgggcc tgttcatgag cgcggtcgat agcgtccggg cggcgggcaa
     1921 gctctggctc aacgaggacg acacgcgcac ctacctcacg cccgaggact ctggcttcgg
     1981 ccgtgtcgac actcctcagg gcaccttctg ggtccatcag cggaacttcg cgcagctgct
     2041 gccccgccgc ctggcctgct ggtacatgga tctgggcggc atcggctggc tcgacggcca
     2101 ggacctatgg gacaacatcg ctcggttgcg agaagtgtac gaggaccggc ttcccacacc
     2161 ggccactttc gcccccgaag tggccgtgat cgtcgatgag gtcagtccct tctacaccca
     2221 gtgcaccagc gcgctgcact cgccgctggt ctaccagatg cgtagccaga tggcccggct
     2281 cggggcgccg gcgcggatcc acctgctgag cgatctggtc gaggggcgtg ttccccgagc
     2341 caaggtctat gtgttcctca actgtttcca cctgagtgac acggcgcggc gggcgatcag
     2401 ccgcaccacg gccggccggg cggccatctg gttctacggc ggcgggttcc tgggcgacga
     2461 cgcggccccg gtcacggcag agggcgagtt cgagcggcgg atgagcgacg ccgtgggctt
     2521 gccgctccgc cgtaccgttg tccagcccgg catagctcga ctcgtcgagg acgaccgcag
     2581 tatcggcact gacctcacac tggagccctg ctgggccgtg gacgagggcg ccggggcgga
     2641 ggtcatcgcc cgctacgccg acggctcgat cgccgcggcc tggcgccaga cgcgtgaggg
     2701 gctgcgcgcc tatgtgggag cgctgcactg cccggccagt gtgctgcgcc ccctgcttcg
     2761 acgggccggc gtgcatctgt acgccaccag cgatgacgtg gtgctggccg atgaccgctt
     2821 cctgggtctc gccgccacca gtgcgggggc gaaacggatc cgactccggc gcccggccac
     2881 ggtgatcgac ctcctgagcg gcgaggtcct gggggcgaac gtcgacgggt tcgagctcac
     2941 catggccctg ggcgagacgc gcctgtttgg cctgcggtag aatggctccc gtctagccaa
     3001 tgcactggaa gaccgtgggg cgctggccgc cccacggtcg tttgacggga gctgctcatg
     3061 tccggcgccg aaccgcgggg cgcgctgaat ctgctcgtcc tggccgacct gcactacatc
     3121 caccatgccc gccaccaatg cggagttccc caacgccgag ctgatatggg gctcgagttg
     3181 gtcacgcgcg ccgttcgtcg cgcccggcgc atcgcggaca tcgacgtcat cgtcctcctc
     3241 ggtgacctcg tcgacaacgg ctgggccgag ggggccgagc gcgacctggc cgagctgcga
     3301 gacgccctcg agaagccagg cctgccggtc atcgtggtgc ccggcaacca cgacggcccg
     3361 cccgagcgcg ttcgggccgt cttcggcgcc gagacgggcg tcggccagat cgccggctac
     3421 cagttcgcca cgtttgcgga cacctatggc cccggcgacc gctgtgtgcg tccggtggca
     3481 gacctggtgc acacgctgtg ccgcgcgcgt caccaggccg accagcccat catcgcgctc
     3541 cagcacaacc cggtccatcc gcccatcgag agcgcctacc cgtacatgct cgaggagcgc
     3601 gcccaggtca tggagctcta tgccgagtcg ggggtcgcgc tctcgctcag cgggcactac
     3661 catccagggc agccaccgac ggtagtgcgc ggcgtcacct atgccacatg cccggcgctg
     3721 tgcgaggcgc cattccggtt catgctggtc cgtatggagg gccggcaggc gaacgtcgag
     3781 accctgtcgc tggccatgga ccctgaaacc ggactcgtgg accaccactg ccacactcac
     3841 tatgcgtact gtgcccagga catcacggcc gagaaggtga tcgcccgggc gcgggacatg
     3901 ggcctggcgc gaactcacct gacggagcac gccggacagc tctacatggc ggccgatgac
     3961 ttctggaagg gtgcgttcgt cacggatcct gacctgtgga tccgcgcgcg cgccgagcgc
     4021 aaccggatgc cagcattccg ggcggagatc gggcccctgc gtcgccccga agtccgctgg
     4081 ggactcgaag tggagtgcga tggcctcggc cgcctgacac tggccgatga ggaccgcgag
     4141 ggctgggatg tcctcgtggg ggccctgcac tacctgcctg ctacggtcgg cgccggcgcg
     4201 atggacccgg cgcggctgac ccgggagttt atgacattct ccgagcatct cctccggcag
     4261 ggcgcccaga ttctggccca cccgttccgc atcatccgaa aagccaaggt acccgtgccg
     4321 gagtggctgt tcgcgccgct ggtcgacatg ctggccagct acggcgcggc ggccgagctg
     4381 aacttccaca cgaaccagcc cgacccacgg ttcttcgcgg cgtgcctcga gcgcggagtt
     4441 cggatctcgc tgggcagcga cagccacgcc atgtacgagg tgggcgactt cgcgccacac
     4501 ctacggctgc tggaggacat cggcgtcacc cgggaccggc tgccggatgt gctgttcggg
     4561 ctctcggcct cctaaggcaa ctccgcttgg ctctgatcgg cgagcacgaa cttgtagcga
     4621 gccgggaggc ggttgcggcg ggacaagacc acctggttcc cgagaaggct cgagtcga
//
LOCUS       DAOSPL010000128         4645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_18548_length_4645_cov_2.958824, whole genome shotgun sequence.
ACCESSION   DAOSPL010000128 DAOSPL010000000
VERSION     DAOSPL010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18548_length_4645_cov_2.958824"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..588)
                     /locus_tag="PLQ54_03595"
     CDS             complement(67..588)
                     /locus_tag="PLQ54_03595"
                     /inference="COORDINATES: protein motif:HMM:NF022216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOS92370.1"
                     /translation="MTPHGDPVSRSGDASEDHPEVILEWSVCPAREHPGRTWAALAMI
                     VVVLGLVGAAFGSPFYVVLACVFLLSALFPYLTRTYYRLDPDGVQVRTLFGRHRRPWD
                     QFRIYRVSGDQILLCTRRTPSRLDTYRGCRLLLSGNEAEVRSALESVGVPYAGQPLEE
                     AGSSCETTDAPGS"
     gene            633..2618
                     /locus_tag="PLQ54_03600"
     CDS             633..2618
                     /locus_tag="PLQ54_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92371.1"
                     /translation="MSTPSVCPTGGKSLEIAQPAGESGFRFWAVCLVALLAAPFNALW
                     IVHMEGVRDLGQPTIVSVFYNVIFIVFVVTSLNHLVRLVSPRRALSQAELLVLYVMLS
                     VLTSPAGLDTLQILTCTITGPYRFGTPESGWQELFFQYLPEGMIVSDPVALNNIWAGG
                     GSLYQPENLNGVLGPMLRWWAFLSALWIAPLGLAVLFRRRWIESERLTFPIVHLPLEM
                     TRPDARLFRSRLMWMAFGLAATIQLLNGLHLIYPVVPQVPVKFQQVPAMNLASQLLDR
                     PWNAIGTFGVAFYPFIIGLALLLPMELPLSCVLFYFFFKAQMIFCSHAGLTQYPEFPY
                     LKEQSFGGYMALLVFSIWAGRSYYRDVLARAVGLLRKPIDRDEPLSYRAAVLLFLAGA
                     AYVIGVGVNQRMALWVSIAFFAQYFVMTCVVGRIRAEMGLPTHEMERLGPAVTMGNGL
                     GKRILGDQNLTSLSLFFGFTRGIRNVPFPHQVEGLKLMERSRGSSRRLLYAMAIAVVV
                     GIGCALWAEFHLAFENGFGARWRDLPGWLSREAWEQLVGWINVPKGIDRGRAEAALIG
                     FVFYFGAMVLRTQCLWWPLHPAGFALSTTYFMDHMWFPFLIAFCAKWTLTRYGGHQIS
                     RGVALFALGLILGDITIGCGWSLYGIARSFNAWQFWP"
     gene            2949..>4645
                     /locus_tag="PLQ54_03605"
     CDS             2949..>4645
                     /locus_tag="PLQ54_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92372.1"
                     /translation="MWHWSVSASAPPPGTMMMWGISGIWKKRDNGAWQSAPNIFIDPA
                     GTKGGTWLTYGDGDQNFPIDVKCSITYTLFGPGGSSQGSAETNTVTATPGNMVTHCTG
                     GNALLILHAPGDEDPNTPGAEEHVLTWNAYHQDWCHSAFANEHMHCGISVLPVTGNPA
                     SPDWQITVPQGDPGDGSYTWEGQRTPPRPSAPKGIYTWLVSASHKYDPDGTGCPVGCG
                     HVAECPPDTDKVGRPATVQSFEYLHLDPEANRLFVRVTYALATDVGECMVKVFTPSFD
                     AVTWAASGDELTAGTHTTDVLEIEGVNDDQGNVVDGDEFCAVVLSSQTAAEGLLNRDR
                     QPKPHVPHGMYGQAQTGSVTLESISFEDDIPMKWAVHPLDGNPVDLGNPEWTRADVRQ
                     DWKLLRPYCTCPRPYDFLPGSPSGACPNHGGAWSGPKRDPAAYVRPSKANRTNNVPLL
                     VTLKGPAGATGTAWATGSLGGVMAFPVEFDSNGDATDQPAHTGAEVTDTVDVLGVNWS
                     WHFQFDETAHQIALGDSEHTVYVTLDQRLAGVPVFLQLVDFSCRWADGGEAAAYVFDA
                     "
BASE COUNT          735 a         1619 c         1455 g          836 t
ORIGIN      
        1 catccacgaa cagctcggcg gccgagatgc tcggagcggc cagcgccagc agggcacatg
       61 acatggctac gagcccggcg cgtctgtggt ctcgcatgac gatcccgcct cctctagggg
      121 ctgcccggcg tacgggacgc ccaccgactc gagcgcggac cgcacttcgg cctcgttacc
      181 tgagaggagc agacggcacc cccggtacgt gtccagccgt gagggcgtcc ggcgagtgca
      241 gagcaggatc tggtcgccgc tgacccggta gatccggaac tgatcccagg gccggcgatg
      301 ccgcccgaat agggtgcgaa cctgcacccc gtcggggtcc agccggtagt acgtccgcgt
      361 caggtagggg aacagggcag acaacaggaa aacacaagca aggaccacat agaaaggtga
      421 gccgaaggcc gcgccgacga gacccaggac gacaacgatc atggcaagcg ccgcccaggt
      481 ccggcccggg tgttcccgag ccggacaaac cgaccactcg aggatgacct ccgggtggtc
      541 ctccgaggcg tcgcccgatc gactcaccgg gtccccgtgg ggtgtcatgc tgtgcgatgc
      601 tccccccaag tcgacgcccc ttccctggcc ccatgagcac tccctccgtc tgccccacag
      661 ggggcaaatc gctcgagatc gctcagccgg cgggcgagtc tggcttccgg ttctgggccg
      721 tgtgcctggt cgcgctcctg gcggccccgt tcaacgcgct ctggatcgtc cacatggagg
      781 gcgtccgcga cctggggcag ccaaccatcg tgtctgtctt ctacaacgtc atcttcatcg
      841 tcttcgtcgt gaccagcctc aaccacctgg tccgccttgt gtccccacgc cgagcgctat
      901 cgcaggccga gctgctagtc ctctatgtga tgctctccgt gctgacctcg ccggccgggc
      961 ttgacacgct ccagatcctc acctgcacca tcaccggccc ctaccggttc ggcacgcccg
     1021 agagcggctg gcaggagctg tttttccagt acctgcccga gggcatgatc gtcagcgacc
     1081 cggtggccct caacaacatc tgggcgggcg gcggcagcct ctaccagccg gagaacctga
     1141 atggtgtcct cggccccatg ctccgctggt gggcgttcct ctcggcgctg tggatcgccc
     1201 cgctgggcct ggccgtgctg ttccgccggc gctggatcga gtccgagcgg ctcaccttcc
     1261 ccatcgtgca cctgccgctc gagatgaccc ggcccgatgc acgcctcttc cgaagtcggc
     1321 tcatgtggat ggccttcggc ctcgccgcga ccattcagct cctgaacggc ttgcacctca
     1381 tctacccggt ggtgccccag gtgccggtca agttccagca ggtgccggcc atgaacctgg
     1441 cgagtcagct cctcgaccgg ccgtggaatg ccatcgggac tttcggcgtc gccttctacc
     1501 cgttcatcat tggcctggcg ctgcttctgc cgatggagct gcccctctcg tgtgtcctct
     1561 tctacttctt cttcaaggcg cagatgatct tctgctccca tgcggggctc acccagtatc
     1621 ccgagttccc ctatctcaaa gagcagtcgt tcggcggcta catggccttg ctggtcttct
     1681 cgatctgggc ggggcgtagc tactaccgcg atgtgctcgc ccgggccgtg gggctgttgc
     1741 gcaagcctat cgatcgcgat gaaccgctct cctaccgcgc tgccgtgctg ctgttcctgg
     1801 ccggcgcggc gtatgtcatc ggcgtcggtg tgaaccagcg catggccctg tgggtctcca
     1861 tcgccttctt cgcccagtac ttcgtgatga catgtgtcgt cgggcgcatc cgcgccgaga
     1921 tgggcttgcc cacgcacgag atggagcgtc ttggccccgc cgtgaccatg ggcaacggcc
     1981 tcgggaagcg catcctgggc gatcagaacc tcacctcgct gtcgctcttc ttcgggttca
     2041 cccgtggcat ccgcaacgtg cccttccccc accaggtcga gggcctgaag ctgatggagc
     2101 gctcccgggg ctcatcgcgc cggctgctct atgccatggc catcgccgtg gtcgtcggca
     2161 tcgggtgcgc gttgtgggcc gagtttcacc tcgcattcga gaacggtttc ggtgcccgct
     2221 ggcgcgacct tcccggctgg ctcagccggg aagcctggga gcaactcgtc ggctggatca
     2281 atgtgccgaa ggggatcgac cgcggccggg ccgaggcggc cctcattggg ttcgtgttct
     2341 acttcggcgc catggtgctc cggacccagt gtctctggtg gcccctgcac ccggcgggct
     2401 tcgcgctgtc gacgacctac ttcatggatc acatgtggtt cccgttcctc atcgccttct
     2461 gcgccaaatg gacgctgacg cgctatgggg ggcaccagat ctcacgcggc gtggccctgt
     2521 tcgccttggg gctgatcctg ggcgatatca ccatcggctg cggctggagc ctctacggca
     2581 tcgcccggag cttcaacgcg tggcagttct ggccgtaggg ccaaaccggg aacctggagc
     2641 ctcaacactc ggtccaaccc cctgatgcgc agcgccgcgc gtggcactga gtgcgggcaa
     2701 cgttatcagt tgcctcgcga agttacctgt gctacgctaa gcgataacgc tcgtgcagcg
     2761 gtaccctctt ctggaggagg tacctcgtca tggtatgcgg gcaccaccgg cttgtctccc
     2821 tccctccctc cctccctccc tccctcccta tcatctcgtt cctatgtgtc tgtggtctga
     2881 ctctccccgt gtccgcggaa cccaccttca cgctcacgct ccaaccgtct tcgtccacgg
     2941 tgtctgacat gtggcactgg tccgtatcgg cgtcggctcc gccgccgggc accatgatga
     3001 tgtggggcat ctccggcatc tggaagaagc gcgacaacgg cgcctggcag agcgcgccga
     3061 acatcttcat cgacccggcg ggcaccaagg ggggaacctg gctgacctac ggcgatggcg
     3121 atcagaactt ccccatcgac gtgaagtgca gcatcacgta caccctcttc ggcccgggcg
     3181 gttcgagtca gggcagtgct gagaccaaca ccgtaacggc cacaccgggc aacatggtga
     3241 cccactgcac gggcggcaac gccctgctca ttctccatgc cccgggcgat gaggacccga
     3301 acactccggg cgccgaggaa cacgtgctga cgtggaacgc ctaccaccag gactggtgcc
     3361 acagcgcgtt cgcgaacgag cacatgcact gcggcatctc ggtgctgccg gtcacgggga
     3421 acccggcatc gcccgactgg cagatcaccg tgccccaggg cgatccagga gacggctcct
     3481 acacctggga gggtcagcgc accccgccca ggccatcggc gcccaagggg atctacacct
     3541 ggctcgtctc cgcctcccac aaatacgacc ccgatggcac cggctgcccc gtgggctgcg
     3601 gtcacgtcgc cgagtgtccg cccgataccg acaaggtggg gcggcccgcc acggtccagt
     3661 cgttcgagta ccttcacctc gacccggagg cgaaccggct cttcgtgagg gtgacctacg
     3721 ccctcgcgac cgatgtcggc gagtgcatgg tgaaggtctt cacgcccagc ttcgacgcgg
     3781 tcacctgggc ggcatcgggc gacgaactga cggcggggac acacaccacc gacgtcctcg
     3841 agatcgaggg cgtcaatgac gaccagggca acgtggtcga cggcgacgag ttctgcgcgg
     3901 tggttctctc gagccagacg gctgcggagg gcctgctcaa ccgcgaccgg cagccgaagc
     3961 cccacgtgcc tcacggcatg tacggtcagg cacagaccgg gagcgtgacc ctcgagagca
     4021 tctcgttcga ggacgacatc cccatgaagt gggctgtgca ccctctcgac ggcaacccgg
     4081 tcgacctcgg gaaccccgaa tggacgcgag cggacgtccg gcaggactgg aagctcctcc
     4141 ggccctattg cacgtgcccg agaccctatg acttccttcc ggggagcccg tcaggggcct
     4201 gccccaacca tggcggcgcg tggtccgggc cgaagcggga tccggccgcg tacgttcggc
     4261 catctaaggc caaccggacg aacaacgtcc cgctgctcgt cacgctcaag ggtccggcgg
     4321 gtgcgactgg tacggcgtgg gctaccggaa gcctcggcgg agtgatggcg ttccctgtcg
     4381 agttcgactc gaacggcgac gccaccgacc agcccgctca cacgggagcc gaagtgaccg
     4441 acacggtgga tgtgctgggt gtgaactgga gctggcactt ccagttcgac gagacggccc
     4501 accagattgc tcttggcgac tcggagcaca cggtctacgt gaccttggac cagcggctcg
     4561 caggcgtacc ggtgttcttg cagctagtgg acttctcgtg ccggtgggcc gatggcggcg
     4621 aggccgccgc atacgtgttt gacgc
//
LOCUS       DAOSPL010000129         4640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_18574_length_4640_cov_3.370774, whole genome shotgun sequence.
ACCESSION   DAOSPL010000129 DAOSPL010000000
VERSION     DAOSPL010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18574_length_4640_cov_3.370774"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLQ54_03610"
     CDS             <1..313
                     /locus_tag="PLQ54_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92373.1"
                     /translation="SAIQRAVQLAGDIELKGTLRSFQAHAQSASSMIESGQGSQAIQE
                     LIAMAQAIAPVEAQKGLDRVAEHLHGAASALARGVPDVSAAELKGARARLWELLEALN
                     G"
     gene            434..4003
                     /locus_tag="PLQ54_03615"
     CDS             434..4003
                     /locus_tag="PLQ54_03615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOS92374.1"
                     /translation="MAEHRPFVHLHVHTEYSLLDGASRIPDLIARAQEYGMPALAITD
                     HGSMFGALDFYAACTQAGIKPLVGCEVYMAARTRFDRDPKEDQRRSHLVLLARDARGY
                     VNLMQMVTEAYLNGFYYKPRIDMDLLREHSEGLIGLSACIEGEVQQALLGGGQEAGRK
                     ACERYLSALGEGNFYLEAMAHGLDEEVRLVPMLERLGRDMKIPIVATNDSHYTSRGDA
                     GAHDVLLCIQTGRTVKDKSGLCFSSDQFWFKTREEMRHAFGREDWLDNTLMVADQCDL
                     RIETGVVHMPRFSGLPEGRTAPEMLRELVAQGLPQRYNPITEEVRQRAEYELEVIIGK
                     HYADYFLIVWDLSRWAHERGIPLSFRGSAGSSVVAYAIGIHDLDPIALNLVFERFLNP
                     ERDDPPDIDLDVPDDTRGEIIRYAVERYGADRVAQVVTFGTLQGRAAVRDTGRALDVP
                     LAEVDRLAKLIPSMATIDEGLEAQAELRQLYQTDTRVAELIDNARAIEGLIRHASTHA
                     AALVIAHKPLSELTPLMRHANDETITTQYSMDALKKIGVEKLDLLGLKTLTVLRNTLM
                     WIERTTGQKVKLDEVPPDDPETFASLSRGETSGVFQMESAGMRKLAVNLRPTSVAHLS
                     ALVALYRPGPMKTGMLDAYVETSNGRRRIRYLHPLLEPILSVTYGVIVYQEQVMQIAR
                     DLAGFSMGEADVLRRAMGKKQVDVMERMRPKFMEGARQRGVPEDTAKALFDTMAEFAG
                     YCFNRPHSEAYARVAFQTAYLKTHYPAQFMAAQLSSYMDDKTKVALYVEEARRLGVAV
                     MPPDVNQSDADFTPNAEGQIRFGLAAVKGVGRAAVGAILEARSAGPFRDIFDFCRRVP
                     STQVTRAVTEALIRCGGFDSLGSRAAQMAVLDMAQAQAQSLYRDRAVGQTSLFGDDDD
                     ALVGTDSQVLPDVPEWTRDQILSAEKELLGLYVTDNPLKDVVRALDGRVDHTCGELAE
                     EAADATVTVGGVITRVREYMTRANKPMMFLTIQDLEGDADIVVPPKTYESVRSLLVMD
                     AAIVVTGRNSPRDAGAPPGSGMAKVVADAIVPADRAPVRFAKRLDDTGAPLEELNGAS
                     DDPTAGFDALQNGGGNGSECGGGGAIHIRLSAADRSLLDRVRQILGACPGDRDVILHV
                     ADGGRPRVFRLPEQYRASWSEDLRRRLGAVVDDAAAVWCAAGT"
     gene            4039..>4640
                     /locus_tag="PLQ54_03620"
     CDS             4039..>4640
                     /locus_tag="PLQ54_03620"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOS92375.1"
                     /translation="MTIRKLSTQALTPAEIGRVLRPALDERYAEPMRQVQEIVDRVRA
                     EGDQAVLEYARRFDTPGLTAADLEVRPEEIDAACASAPAEHIAAIRRACDHVRRFHAH
                     RVPQSWQHTFDGATLGQRVLPLDRVGIYVPGGTPLPSSLYMAAVPARVAGVREVVVAC
                     PARRDGTLHPLVLVAARLAGVDRVFRVGSAWAVAALAFGT"
BASE COUNT          771 a         1491 c         1629 g          749 t
ORIGIN      
        1 gagcgccatc cagcgggcgg tgcagctcgc gggggatatc gagctgaagg gcaccctgag
       61 gagttttcag gcacacgcgc agagtgcatc gagcatgatc gagtccgggc agggctctca
      121 ggccatccag gagctcatcg ctatggctca ggccatcgcg ccggtcgagg cccagaaggg
      181 tctggaccgg gtggcggagc acttgcacgg agccgccagc gctttggcac gcggtgttcc
      241 ggacgtgtcg gcggccgagc tgaagggcgc gcgcgcacgc ttgtgggagc tgctcgaggc
      301 tctgaacggc tagaccggca tcgcctgctt ccatcccgcg gtggcgcctc ggcaactggg
      361 gccgctccgg tccgtttgcc aggactgacg tgactgcgac tcgctggacc gcgccgcggt
      421 acggaggcac agcttggccg aacacagacc cttcgttcac cttcacgtcc acacggagta
      481 cagcctcctc gacggtgcgt cccgcattcc cgatctgatc gcccgggccc aggagtacgg
      541 tatgccggcc ctggccatca ccgaccacgg gtccatgttc ggtgccctgg acttctatgc
      601 ggcgtgcact caggctggca tcaagcccct ggtggggtgc gaagtctata tggccgcccg
      661 tacccgtttc gaccgcgacc cgaaggagga tcagcggcgg tcgcacctgg ttctgctcgc
      721 ccgggacgcg cgcggctatg tgaatctcat gcagatggtc accgaggcgt acctgaacgg
      781 gttctactac aagccccgca tcgacatgga tctgctgcgt gagcatagcg aggggctcat
      841 cggcctgagc gcctgcattg agggcgaggt ccagcaggcg ctgttgggtg gagggcaaga
      901 ggcgggccgc aaggcgtgtg agcgctatct cagcgcgctg ggcgaaggca acttctacct
      961 cgaggccatg gctcacggac tggacgaaga ggtgcgtctg gtaccgatgc tcgagcgcct
     1021 cgggcgcgat atgaagatcc ctatcgtcgc caccaacgac agccactaca cctcgcgtgg
     1081 cgacgccggc gcgcacgatg tgctgctgtg catccagacc ggccgcacgg tgaaagacaa
     1141 gtcaggcctg tgcttcagca gcgaccagtt ctggttcaag acgcgcgagg agatgcgtca
     1201 cgcctttggc cgcgaagact ggctggacaa caccctcatg gtggccgatc agtgcgatct
     1261 gcggatcgag accggtgtcg tgcatatgcc gcgcttctcg ggactgcccg agggccggac
     1321 agccccggag atgctccgcg agctcgtggc tcaagggctg ccccagcggt acaaccccat
     1381 caccgaagaa gtgcggcagc gcgcggagta tgagctcgag gtgatcatcg gtaagcacta
     1441 cgccgattac ttcctcattg tctgggatct gtcgcgctgg gcgcacgaac ggggcattcc
     1501 cctgtcattc cgcggctccg ccgggtcgag tgtcgtggcg tatgccattg gcattcacga
     1561 cctggacccc atcgcgctga acctggtctt cgagcgcttt ctgaaccccg agcgtgacga
     1621 tccgcccgac attgacctcg acgtgcccga tgacacgcgg ggcgagatca tccgctacgc
     1681 ggtggagcgt tacggggccg accgcgtggc tcaggtggtg accttcggga ctctgcaagg
     1741 ccgagcggcc gtgcgtgata ccggccgggc cctggacgtg ccgctggccg aggtggatcg
     1801 tctggccaag ctcattccca gcatggccac catcgatgag ggactcgaag cccaagcgga
     1861 gctgcgacag ctgtaccaga ccgacacccg agtggcggag ctgatcgaca acgcccgggc
     1921 catcgagggg ctgattcggc atgcctcgac ccacgcggcc gccctggtca ttgctcacaa
     1981 gccccttagt gagctaacac cgctcatgcg gcatgccaac gacgagacca tcaccaccca
     2041 gtactcgatg gacgcgctga agaagatcgg cgttgagaaa ctggacctgc tgggtctcaa
     2101 gacgctgacg gtgctccgca acaccctgat gtggatcgag cggaccacgg ggcagaaggt
     2161 gaagctcgac gaggtgccgc cggacgaccc cgagacattc gcttccctct cgcgaggcga
     2221 gacctcgggc gtgttccaga tggagagcgc cgggatgcgg aagttggcgg tgaacctgcg
     2281 gcccaccagt gtggcgcacc tctcggccct ggtggccctc taccgaccgg ggccgatgaa
     2341 gaccggcatg ctcgatgcct atgtcgagac atcgaacggc cgcaggcgga tccggtacct
     2401 ccatcccttg ctggaaccca ttctgtctgt gacctatggc gttatcgtgt atcaggaaca
     2461 ggtcatgcag atcgcccggg acctggccgg cttctccatg ggtgaggccg atgtgttgcg
     2521 ccgcgccatg gggaagaaac aggtcgacgt gatggagcgc atgcgcccca agttcatgga
     2581 gggagcgcgc cagcgcggcg tgccggagga cacggccaag gcactgttcg acaccatggc
     2641 ggagttcgcg gggtactgct tcaaccggcc gcacagcgag gcatacgcgc gggtggcctt
     2701 ccagacggcc tatctcaaga cccactaccc ggcccagttc atggccgcgc agctctcgag
     2761 ctacatggac gacaagacca aggtggcgct ctatgtggag gaggcgcggc gtctgggcgt
     2821 ggcggtcatg ccgccggatg tcaaccagag cgacgcggac ttcaccccga acgccgaggg
     2881 acagatccgc ttcggcctcg ccgccgtgaa aggcgtgggg cgcgccgccg tgggggccat
     2941 tctcgaagcg aggtctgctg ggccgttccg cgacatcttc gacttctgcc ggcgggtgcc
     3001 gagcacccag gtgacccggg cggtgaccga ggcgctcatc cgctgcggcg gcttcgactc
     3061 gctgggctcc cgcgcggccc agatggccgt gctcgacatg gcgcaagcgc aggctcagag
     3121 cctgtatcgt gaccgggcgg tcggtcagac ctcgttgttt ggcgatgacg acgatgcgct
     3181 ggtggggacg gactcgcagg tactgcccga cgtgcccgag tggacccgcg accagatcct
     3241 ctcagccgag aaggagctgc tgggcctgta cgtcacggac aacccgctca aggatgtggt
     3301 ccgcgccctc gatgggcgag tggatcacac gtgcggcgaa ctcgccgagg aagcggcgga
     3361 cgcgaccgtc accgtgggcg gtgtcatcac gcgtgtgcgg gagtacatga ctcgcgcaaa
     3421 caagcccatg atgtttctga ccatccagga cctggaaggc gacgcagaca tcgtcgttcc
     3481 gcccaagacg tacgagtccg tgcggtcgct gctcgtgatg gacgccgcca tcgtcgtgac
     3541 ggggcgcaac tcgccccgag acgcgggcgc gccgcccgga tcgggaatgg ccaaggtggt
     3601 cgccgacgcc atcgtgccgg ccgaccgggc gcccgtccgg ttcgccaagc gcctcgacga
     3661 taccggggcg cctttggagg agctgaatgg cgcaagcgac gacccgaccg cggggttcga
     3721 tgcgctccag aacggcggcg gcaacgggag cgaatgtggc ggcggcgggg ccatccacat
     3781 tcggctgagc gcggccgacc ggtcactgct ggaccgtgtg aggcagatac tgggcgcgtg
     3841 tccgggggac cgtgatgtga ttctccacgt ggccgatggc ggacgtcctc gggtcttccg
     3901 gttgcccgag cagtaccgcg cgtcgtggag tgaggatctg cggcgccgtc tgggtgccgt
     3961 ggtggacgat gccgctgccg tgtggtgcgc ggcgggcacg tgaggccggt tggcccgtcg
     4021 ggcccagaag gagtcgcagt gaccatccgc aagctatcca cacaggcgct gacgccggcg
     4081 gagatcgggc gggtgcttcg tccagctctg gacgagcggt acgccgagcc catgcgccag
     4141 gtgcaagaga tcgtggatcg cgtccgcgcc gaaggcgacc aggccgtgct cgagtacgcg
     4201 cgccgcttcg acacgccggg tctgacggcg gccgacctcg aggtccgacc cgaagagatc
     4261 gacgcggcat gcgcgtcggc gccggccgag cacatcgccg cgatacggcg cgcctgcgat
     4321 cacgtccggc ggttccacgc gcaccgggtg ccgcagagct ggcagcacac gttcgacgga
     4381 gcgaccctgg gccaacgggt gctgccactg gatcgagtgg gcatatacgt tcccggaggc
     4441 acgccactcc cgtcgtcgct gtacatggcg gccgtaccgg cccgtgtcgc cggtgtgcgc
     4501 gaagtggtcg tcgcgtgccc cgcgcggcgg gacgggacgt tgcacccgct ggtgctcgtg
     4561 gccgcgcggc tggcgggtgt ggaccgggtc ttccgggtgg gcagcgcctg ggccgtggcg
     4621 gcgctggcat ttgggaccga
//
LOCUS       DAOSPL010000130         4638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_18583_length_4638_cov_3.141174, whole genome shotgun sequence.
ACCESSION   DAOSPL010000130 DAOSPL010000000
VERSION     DAOSPL010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18583_length_4638_cov_3.141174"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PLQ54_03625"
     CDS             complement(<1..836)
                     /locus_tag="PLQ54_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOS92376.1"
                     /translation="MLHRYDSYGWLRGLNCIPSWGARIEQAWWAYSPDQFRAEMALAR
                     TIHSNCIRLWIEFSAWMAEPEAVTASFMDAIRGIDEAGMKAMPCLFNRWHDWRYDYGG
                     TCIEHLFRDWVPQVEYVQALVTPLAADDRVLAWDLCNEPQAFGREKPEDIKEFEWLST
                     IAAAVRAAGAQQPITIGTMVGSNITNLAELMDVLCGHPYARTRDAMVQAVTELKAIQE
                     AHGKPLLVNECVPGCLDDFRRAEAARYSSELLSEAGFGWMGWSIKEGKAISTRRDRYD
                     ANG"
     gene            1014..2510
                     /locus_tag="PLQ54_03630"
     CDS             1014..2510
                     /locus_tag="PLQ54_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92377.1"
                     /translation="MLLTMSHAVIVSSVLVPADGTYVCDIGSRLELFVDDWLIERLEG
                     GADRRLHHPEPREVALVLDRSWEGNAVGYVTVFRDGEGFRMYYRGAHVVYTPEASTET
                     HPEYTCLAESPDGVHWERPVLGLHEIEGSRDNNVVVAPAEGWGLATHNFTPFIDPRPG
                     VPAAERYKALGGVGQGAGGVYAFASADGVRWRKLHDGPVITEGDFDSQNLAFWDASRG
                     EYRFYGRKFRDGRDIITCTSPDFVSWTTPQFVDYSPGRVSELYTNQVWPYPRAPHIWI
                     GFPTRYIDRGWTEAARHLPQIEYRELRAKASVREGTALTDGMLMSTRDGQHFDVWPES
                     FIRPGLRTAGNWFYGDNYQCLGLIETPSAIEGAPPELSFFATENNLQKAPARLRRFAI
                     RVDGFVSIQAPLSGGELLTRPLLFAGSRLLLNYSTSAAGSVQEEIQDTKGQPMPGFSL
                     AESEALYGDSLEQAVVWKGSPDLAALSGQAVRLRFVLRDADLYSLRFG"
     gene            2527..2772
                     /locus_tag="PLQ54_03635"
     CDS             2527..2772
                     /locus_tag="PLQ54_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92378.1"
                     /translation="MPIAWFMPLTAVVPLRGQPLHATGLIGIQRVDEVWRPVDAAGER
                     LIPIGANHIGCVAGHCRAVVGVRSTSWLNRRRRAKQR"
     gene            complement(2792..4429)
                     /locus_tag="PLQ54_03640"
     CDS             complement(2792..4429)
                     /locus_tag="PLQ54_03640"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOS92379.1"
                     /translation="MNGAELLVRELQARGIDCVFTLTGNGLAPLMAACGRLGMRVIDT
                     RNEQAAAYQADGIGRMTRRLGVCAVSSGVAHVNALSGVANAWYDGSPMLLITGGSESD
                     TRGKGHFQDMDTAGLAAPLCKYCTYVSRVDQLISHMDMAIEAATTGRPGPVHLTVPVD
                     VLVSETTAQLSGKTTAAVVSGGPPDAAAVAEAAHVLASSARPFVVAGSGVFYADAASE
                     LASLARALGAPVVVPIWDRGAVEQPLPEFVGVLGAASGQARILPDADAVLLVGARVDY
                     RVGYLEPFAVRPDAQVVRISADADELTQGVEPHVAVLGDPRRAMEGIRQRLAARGHVA
                     DGAWLAEARRRHREFLAGQPWRAALTAPSGMTTGSDIIEAIREVLTDQTYFLIDGGNI
                     GQWVHMAMADRYPGRWMTCGRSAVIGWGIPTAAAVRAYDPEAPIVLLSGDGSATFTIA
                     ELECAARQQLPFVAVVADDSAWGIVLSGIRQAGGPSVGAELGPIDFVKVAEGLGCRGL
                     RVDEPSGIADAIRRGLTETAVPTVIHVPMTTGGPADR"
BASE COUNT          708 a         1594 c         1583 g          753 t
ORIGIN      
        1 ccattggcgt cgtagcggtc tcgccgggtc gagatggcct tgccctcctt gatgctccag
       61 cccatccagc cgaagccggc ctcggagagc agctccgagg agtaccgcgc ggcctcggcc
      121 cggcgaaaat catcgaggca gccggggacg cactcattca ccagcagcgg cttgccgtgg
      181 gcctcctgga tggccttcag ctcggttacg gcctgaacca tggcgtcccg cgtccgggca
      241 taggggtgcc cgcagagcac atccatcagc tcggccaggt tcgtgatgtt gctgcccacc
      301 atggtaccga tggtgatggg ctgctgcgcc ccggcggcgc ggaccgccgc ggcgatagtg
      361 ctgagccact cgaactcctt gatgtcctcc ggcttctcgc gcccgaaggc ctgcggctcg
      421 ttgcacaggt cccacgccag cacgcggtcg tcggcagcca gcggggtcac cagcgcctgc
      481 acgtactcga cctgcggcac ccagtcgcgg aacagatgct cgatgcaggt gccgccgtag
      541 tcgtaccgcc agtcatgcca gcggttgaac aggcacggca tggccttcat gccggcctca
      601 tcgatgcctc ggatggcatc catgaagctg gccgtcacgg cctcaggctc ggccatccac
      661 gcgctgaact cgatccagag ccggatgcag ttcgagtgga tggtccgcgc cagggccatc
      721 tcggcacgga actggtccgg gctgtaggcc caccaggcct gctcgatccg cgcgccccat
      781 gaggggatgc agttgaggcc cctgagccat ccgtagctgt cgtagcggtg cagcatgacc
      841 atcagctccc cggttcgggc gatgcgtggg gagttcgccg gcgggcgccc tcgagccctc
      901 ttgccgggcc gcgcagcgcg ctggacgcag gccgaaaggg gcttcgcgag gggccgcgct
      961 ggcttgggcc gaactccggc gcccaggccc tacccaagag aggtgatctc cccatgctcc
     1021 tcacgatgag ccatgcggtg atcgtatcga gcgtgctcgt gccggcagac ggcacctatg
     1081 tgtgcgacat cgggtcgcgg ctcgagctgt tcgtcgacga ctggctgatc gaacggctcg
     1141 agggcggtgc cgacagacga ctgcaccacc ccgaaccgcg cgaggtggcc ctggtgcttg
     1201 accggtcgtg ggagggcaac gcggtaggct acgtgaccgt cttccgggat ggggaagggt
     1261 tccgtatgta ctaccggggg gcccacgtcg tctacacgcc ggaggcgtcg acggagacac
     1321 acccggagta cacatgcctg gccgagagtc cagatggcgt ccactgggag cgcccggttc
     1381 tgggactgca cgagattgag ggctcgcgcg acaacaatgt ggtcgtggcc ccggcggaag
     1441 gctggggctt ggccactcac aacttcacgc cattcatcga cccgcggcct ggggtgccgg
     1501 ccgcagagcg ctacaaggcc ctgggcggcg ttggtcaagg cgcggggggt gtctatgcgt
     1561 tcgcgtctgc cgacggcgtc cggtggcgca agctgcatga tgggcccgtg atcacggagg
     1621 gcgatttcga ttcccagaac ctggccttct gggatgccag ccgtggtgag taccgcttct
     1681 acggacgcaa gttccgcgac gggcgcgaca tcatcacatg cacgtcgcct gacttcgtga
     1741 gctggaccac gccgcagttc gtcgactact cccccggccg agtgtccgag ctctacacga
     1801 accaagtctg gccctacccc cgggctcccc atatctggat cggtttcccc acgcggtaca
     1861 tcgaccgcgg gtggacggag gcggcgcggc atctgccgca gatcgaatac cgtgagcttc
     1921 gagcgaaggc ttcggttcgc gaggggaccg cactgaccga cggcatgctc atgtcgaccc
     1981 gggacggcca gcacttcgat gtgtggcccg agtctttcat ccgaccgggc ctgcgcacgg
     2041 cgggcaactg gttctacggc gataactacc agtgcctggg gctcatcgag accccgtcag
     2101 ccatcgaggg tgcaccgccc gagctgtcgt tcttcgccac cgagaacaac ctacagaagg
     2161 cgcccgcgcg gttgcggcgg ttcgcgatcc gcgtcgatgg tttcgtgtcg atccaggcac
     2221 cgctttcggg cggtgagctt ctcacccggc cactcctctt cgcgggcagc cggctgttac
     2281 tgaactactc gacgtcggcc gccggaagcg tccaggagga gatccaggac accaagggcc
     2341 agcccatgcc cgggttctcg ctcgcggagt ctgaggcgct ttacggcgat tcgctcgaac
     2401 aggctgtcgt gtggaagggg agcccggacc tggcagcgct gtccggccaa gcggtgcgat
     2461 tgcggttcgt gcttcgggat gcggatctgt actcgctccg gtttgggtag tcccggagcc
     2521 agtcgcatgc ccatcgcctg gttcatgccc ctgacggccg ttgtgccgct gagggggcag
     2581 ccactccatg ccacagggct catcgggatc cagcgggtgg acgaggtgtg gcggccggtc
     2641 gacgcggcgg gtgagcgcct catcccgatt ggtgccaacc acatcggctg cgtggccggc
     2701 cactgccggg cggtggtcgg cgtgcgctcg acgtcctggc tgaaccggag aaggcgcgcc
     2761 aagcagaggt gacaccggct tgcctgcacc cctaccggtc cgccggtccg ccggtggtca
     2821 tgggcacatg gatgaccgtg ggcaccgcgg tctcggtgag gcctcgccgg atcgcgtcgg
     2881 cgatgcctga gggctcgtcg acgcgcaggc cccggcaccc gaggccctcg gcaaccttga
     2941 cgaagtcgat cggtccgagc tctgccccaa cggagggccc gccagcctgg cggatgccgc
     3001 tcagcacgat cccccaggcg gagtcatctg cgacgaccgc cacaaagggg agctgctggc
     3061 gcgccgcaca ctcgagctcg gcgatggtga acgtcgccga gccgtcgccc gacagcagga
     3121 cgatcggcgc ctcggggtcg tacgcgcgca cggccgcggc cgtggggatg ccccatccga
     3181 tgaccgcgct acggccgcag gtcatccacc ggcccgggta gcggtcggcc atggccatgt
     3241 ggacccattg gccgatattg ccgccgtcga tgaggaagta ggtctggtcc gtgagcacct
     3301 cccggatggc ttcgatgatg tccgaacctg tggtcatgcc ggatggcgcc gtcagggcgg
     3361 cgcgccacgg ctgtccggca aggaactcgc ggtgccgccg acgtgcttcc gccaaccagg
     3421 ctccgtcagc cacatgaccc cgggcggcca gtcgctggcg gattccctcc attgcgcgtc
     3481 tcgggtcacc caggacggcc acgtgcggtt caacgccctg tgtcagctcg tcggcgtccg
     3541 cgctgatccg gactacctgg gcgtcagggc gcaccgcgaa cggctccaga tacccgaccc
     3601 gatagtcaac ccgggcgccc acaagcagaa cggcgtcggc gtcggggagg atccgcgcct
     3661 gcccactcgc cgccccaagc acgcccacga actcgggaag cggctgctcg acagcaccgc
     3721 ggtcccagat gggcaccacc accggtgcgc cgagcgctcg agccagggac gcgagttcgc
     3781 tggcggcatc cgcgtagaag actcccgatc ctgccacgac gaaaggtcgg gcagaggagg
     3841 ccagcacatg ggccgcctct gccacggccg ccgcgtccgg cgggccgccc gagacgaccg
     3901 cggctgtcgt cttccccgac agctgagccg tggtctccga caccagcacg tcgacgggca
     3961 cggtcaggtg cacggggcca ggtcggcccg tggtggccgc ctcgatggcc atgtccatgt
     4021 ggctgatgag ctggtctacc ctcgagacat acgtgcagta cttgcacagt ggcgcggcaa
     4081 gtccggccgt gtccatgtcc tggaagtggc ccttgccacg ggtatccgat tcgctcccgc
     4141 cggtgatcag cagcatgggc gagccgtcgt accatgcgtt ggcgaccccg gacagcgcgt
     4201 tgacgtgggc gacgccactg gagacggcac agacgccgag ccggcgtgtc atccgtccga
     4261 tgccgtcggc ctggtaggcc gcagcctgct cgtttcgggt gtcgatgacc ctcatgccca
     4321 gccggccgca cgccgccatc agcggcgcca ggccgttgcc ggtcagggtg aacacacagt
     4381 caatgcctcg ggcctgcagc tcgcggacca acagctcggc gccgttcaca gctcgtcctc
     4441 catgcggggt tagggggcac ccccacgtgc ccggggcacc caccgcggga gcggcgggcg
     4501 ctgcgccatc cggcccgctc gtcgggtccg catgacgtag ggccgtcgtg catcgctatc
     4561 cgtacctttg cgcgagcgct gccagcagtg cgtccttgtg gtggctcgcc gcatacgctc
     4621 gcagttcttc cacatgtg
//
LOCUS       DAOSPL010000131         4630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_18627_length_4630_cov_3.083716, whole genome shotgun sequence.
ACCESSION   DAOSPL010000131 DAOSPL010000000
VERSION     DAOSPL010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18627_length_4630_cov_3.083716"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PLQ54_03645"
     CDS             complement(<1..531)
                     /locus_tag="PLQ54_03645"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HOS92380.1"
                     /translation="MRRAPRGLPGLCALASALVCASCWLQAAEGPGGFAAVTVRADGR
                     HLRTSVPADATIGDALRAINVAVGPCDRVKPRVGTRVHDGLCIAVFRVRVQDSVEQVT
                     VPYVVEIREDRSLPSGRVRLEDGSWCDGLVRRTTRTYFLDDGTTERFIVSEEALRPMK
                     PKVIVVGCKGGSPSRAS"
     gene            complement(614..1867)
                     /locus_tag="PLQ54_03650"
     CDS             complement(614..1867)
                     /locus_tag="PLQ54_03650"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOS92381.1"
                     /translation="MTQSESLRAEVAKCNRCGYCQETCPVFAVTGVESSVARGRIALV
                     RRILEGRAELSEEMDHPLFECLLCRGCVSVCAAGAETDRIIVESRAAWRRQHGSSAAW
                     TAARETLLDRGALIRAARVAFSASRLGLPRLARATGLLRLFPPGLQAAAELVPQPPAR
                     FLTDLWQELDLVPAHASRRVGYFVSCGMNLVQPRAATDSVRSLAALGCAVEPLDNCCC
                     GLPAYVSGETGTARLAAERNLLAIEERPGLDAVVTDCASCSSHLRRMPELVSPDLRER
                     AERVAARVLDYTQIVGQASWPTALRPVARRVTFHDPCHLARHQDLAQWPRTILRVIPG
                     LDYVELPEADWCCGGAGTYSAAHAEVAGSILARKWGHVQSTDADTVVTACPSCMIQLE
                     YGARRAGSRIRVRHISSVVAEALGC"
     gene            complement(1921..2538)
                     /gene="lexA"
                     /locus_tag="PLQ54_03655"
     CDS             complement(1921..2538)
                     /gene="lexA"
                     /locus_tag="PLQ54_03655"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003385736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOS92382.1"
                     /translation="MGSLTRRQESVLQAIRDHIRQEGYPPTVREIGERLGLSSSCTVQ
                     KHLEALIRKGIIRRDGSKSRTIEIVGEPAPPPPMSPDVVFVPLVGRVAAGQPMTAVEY
                     IEGYLPIPQALVRGDDVFMLRVHGDSMINAGIYDGDLVVVRRQNTADNGDMVVAMTAD
                     GDATVKYFHRENGHFRLQPANDAMEPLIVPDVEILGKAILAIRRL"
     gene            2710..3603
                     /locus_tag="PLQ54_03660"
     CDS             2710..3603
                     /locus_tag="PLQ54_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92383.1"
                     /translation="MPFRPAEASDDIEAPETNLGPYVLALLLVACAACAVGLWQSWPR
                     GAEICALAVGDADALVVRVAGRRAVLIDGGGADQGGSRSRALLSHFMESGIKRLDAVI
                     LTGSRARDVGGIPSVLREMPVGLVLAPADLRATPHGRIVALLCRATRTPIGVLADGQV
                     IHLGGGAEIRLRTWRVDRTSTMVGVRVMHGRASAEILNSVTSAGIRVLRGSPPPRADV
                     LKVPAQGQTWANPTELLESVGPRVAVVSRAGTSTAKQWRDVIEDLLASGVDVWMTDTD
                     GAITVRLRNGTVAVHGFRRRR"
     gene            3615..4577
                     /locus_tag="PLQ54_03665"
     CDS             3615..4577
                     /locus_tag="PLQ54_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOS92384.1"
                     /translation="MDEGQIRSASAKAERILHEVETVIVGKPHAVRQVLVGLIAGGHV
                     LLEDIPGVGKTMLARAMSRAIGGTFRRIQFAPDLLPSDITGSNLYNQSTHEFVFRPGP
                     IFANIVLADEINRATPKSQASLLEGMEESQVTVDGVTYDLPSPFFVMATANPIEFRGT
                     FPLPETQMDRFLLRIGMGYPSHDDEIEVLSRQTRGHPIAHVNAVATVEDIQMIRAVAQ
                     EVHVEPSVKDYMVSLVEATRKNPRSLLGASPRGSLGLFKASRALALLDGRSFVTPDDV
                     KRIAPMVLCHRISVDPQLAVQGVTPEHVVIEALEQVPVPLAAAS"
BASE COUNT          739 a         1467 c         1687 g          737 t
ORIGIN      
        1 gctggcccgt gacggtgacc cgcccttgca gccgacaacg atcaccttcg gcttcatggg
       61 ccggagggct tcctcggaca caatgaaccg ctcggtggtg ccgtcatcga ggaagtaggt
      121 tcgggtcgta cggcggacca ggccgtcgca ccaactgccg tcttcgaggc ggacccggcc
      181 gctcggcagg ctccggtctt cacgaatctc gacgacatag ggcacggtga cctgttcgac
      241 cgagtcctgc acgcggaccc ggaacacagc gatgcagagg ccatcgtgaa cgcgggtgcc
      301 gactcgaggc ttgacgcggt cgcatggccc cacggcgaca ttgatggcgc ggagggcgtc
      361 gccgatagtg gcatcggccg gcacggatgt gcggagatgc cgcccgtcgg cgcggacggt
      421 gacggcggcg aacccaccag ggccctccgc cgcctgcagc cagcatgagg cgcagaccag
      481 agccgaagcc agcgcgcaca gcccgggcag ccctcgcggg gctcggcgca tcgcatagcc
      541 tcccggggga tatcacccca gcggcgcaag ggtatgcgcg cacagtctgg atgtcaagat
      601 gcaccggaag ccgtcagcac ccgagggctt cggcgacgac cgaggagatg tgcctgaccc
      661 ggattcggga cccggcccgc cgagcgccgt actcgagctg gatcatacag gacgggcatg
      721 cggtgaccac ggtgtccgca tcggtggact gcacatggcc ccacttgcga gccaggatgc
      781 ttccggccac ttcggcgtgg gcggcgctgt atgtcccagc gccgccgcag caccagtcgg
      841 cctcgggcag ttcgacatag tcgaggcccg ggatcacgcg caggatggtc cggggccact
      901 gcgccaggtc ctggtgccgg gccagatggc aggggtcgtg gaaagttacc cgccgggcca
      961 cggggcggag tgccgtcggc caactggctt ggcccacgat ctgggtgtag tccaggacgc
     1021 gggcggccac tcgctcggcc cgctcacgca gatcgggcga gacgagctcg ggcatgcgcc
     1081 tgaggtgcga gctgcagctc gcgcagtcgg tcaccacggc atcgaggccg ggccgttcct
     1141 cgatggcgag gaggttacgc tcggcggcga gccgcgcagt gccggtctcg ccgctgacat
     1201 aggcgggcag gccacagcag cagttgtcga ggggctcgac cgcgcatccc agagccgcca
     1261 gggagcggac gctgtcggtg gcggcccggg gctggaccag gttcattccg caggagacga
     1321 agtagccgac gcgtcgcgat gcgtgcgccg gtaccaggtc gagctcctgc cagaggtcgg
     1381 tgagaaagcg cgcgggcggc tggggcacga gctcggccgc cgcctgaagg ccgggtggga
     1441 acaggcgcaa gagaccggtg gcacgggcca gccgcggcag gccgagcctc gatgcgctga
     1501 aggcgacacg ggcggctcgg atcagggcgc cgcgatccag gagggtctcg cgcgccgccg
     1561 tccaggcggc cgagctgccg tgttgccggc gccaagcggc ccgagactcg acgatgatgc
     1621 ggtcggtctc tgcgccggcc gcgcagaccg acacgcagcc gcgacacaga aggcactcga
     1681 acagagggtg gtccatctcc tccgagagct ctgcccggcc ctcgaggata cggcgcacca
     1741 gggcgatgcg cccccgagca accgatgact cgacacccgt gacggcgaac acggggcacg
     1801 tctcctggca gtagccgcac cggttgcact tggccacttc ggcgcggaga gactcggact
     1861 gggtcataaa gtaggcccct tgggatccgg gttcgtggga cgcgttcggg tcgacggccg
     1921 ctacagccga cggatggcga gaatggcctt gccgagtatc tcaacgtccg ggacgatgag
     1981 gggctccatg gcgtcgttgg ccggttggag gcggaagtgg ccgttctcac ggtggaagta
     2041 cttgacggtg gcatcgccgt cggcggtcat ggccaccacc atgtcgccat tgtcggccgt
     2101 gttctgcctg cggaccacta cgaggtcgcc atcgtagatt ccagcgttga tcatactgtc
     2161 gccgtggacc cggagcatga agacgtcatc gccccgcacc agcgcctgag ggatgggcag
     2221 gtagccttcg atgtactcga cggccgtcat gggctggccc gccgccacac ggccgaccag
     2281 gggcacgaag accacgtcgg gtgacatcgg gggcgggggg gcgggctcgc cgacgatctc
     2341 gatggtccgt gacttgctgc cgtcacggcg gatgataccc ttgcggatga gggcctcgag
     2401 atgcttctgg acggtacagc tggagctgag gccgaggcgc tcaccgatct cacgcacggt
     2461 cggggggtag ccctcttgcc ggatgtggtc tcggatggcc tggagcacgc tttcctggcg
     2521 gcgggtcaat gatcccacag cacatgcctc ctcccaggcg ggacgtcccg tggtgggttc
     2581 agggatgccg ggccgggtct ggctgggtcg ttgtcgcact ttcatcatac caaacggaag
     2641 cttgcatgca aacagatgtt tcttgccgtc attgcggcaa tccaccgcat attctaggat
     2701 atgctcacta tgccattccg tccggccgag gcgtccgatg acatcgaggc gcccgagacc
     2761 aatctggggc cgtatgtact ggcactgctg ctggtcgcgt gcgccgcgtg cgcggtcggg
     2821 ctctggcagt cctggccccg gggcgccgag atctgcgctc tggcggtggg cgatgccgat
     2881 gcactcgtcg ttcgggtggc cggccggcga gcggttctga ttgacggcgg cggcgctgac
     2941 cagggcgggt cccggtcgcg agcgctgctg tcccacttca tggagagcgg catcaagcgg
     3001 ctggatgcgg tgatcctcac gggttcacgg gcccgggatg tgggcggaat ccccagcgtg
     3061 ctgcgcgaga tgccggtcgg cctggttctt gcccccgctg acctgcgggc cacaccccat
     3121 ggccgcatcg tcgcactgct ctgccgtgcc acacggacac cgatcggagt tctggctgat
     3181 gggcaggtca tccacttggg tggcggggct gagattcggc ttcgcacctg gagggtggac
     3241 cggacgtcta caatggtcgg ggtgcgggtg atgcacgggc gggcatcggc ggagatcctc
     3301 aactcggtga cgtcggccgg catccgagta ctcaggggca gcccaccgcc ccgtgcggac
     3361 gtcctgaagg taccggccca gggccagacc tgggcgaacc cgacggaact gctggagtcc
     3421 gtgggtccgc gcgtggcggt ggtatcccgg gcgggcacga gcacggccaa gcaatggcgg
     3481 gacgtcatcg aggatctgct cgcctcgggt gtggacgtgt ggatgacgga cacggacggc
     3541 gcgatcaccg tgcggctccg gaacgggacc gtcgcggtcc atggcttccg gcgccgccgc
     3601 tgagggggat ggtcttggac gagggccaga tacgcagtgc gagtgccaaa gcggagcgga
     3661 ttctccacga ggtggagacg gtaatcgtcg gcaaacccca tgccgtccgg caggttctcg
     3721 tggggctcat cgccggcggg cacgtgctgc tggaggacat ccccggggtc ggcaagacca
     3781 tgctggcacg ggcgatgtcc cgcgcgattg gcgggacctt ccgccgcatt cagttcgccc
     3841 cagacctgct tccatcggac atcacgggca gcaacctcta caaccagagc acccacgagt
     3901 tcgtgttccg gcccgggccg atcttcgcga acatcgtgtt ggccgacgag atcaaccggg
     3961 cgacgcccaa gtcgcaggcg agtctcctgg agggaatgga ggagtcgcag gtcacggtcg
     4021 atggtgtgac ctacgatctg ccatcgccgt tctttgtgat ggccacggcg aaccctatcg
     4081 agttccgggg cacgttcccg ctccccgaga cacagatgga ccgtttcctg ctgcggattg
     4141 gcatggggta tcccagccac gatgacgaga tcgaggtgct gtcgcgtcag acccgggggc
     4201 atccgatcgc ccacgtgaac gcggtggcga cggtggagga cattcagatg atccgcgcgg
     4261 tggcccagga ggtgcatgtg gagccgtcgg tcaaggacta catggtgtcg ttggtcgagg
     4321 cgacacggaa gaacccccgg tcgctgctgg gggcgtcccc gcgcggctca ctgggtctgt
     4381 tcaaggcgtc gagggcgctg gccctgctgg atggccggtc gttcgtgacg cccgatgacg
     4441 tcaagcgtat cgcccccatg gtgctgtgtc accggatctc ggtggaccct cagctggcgg
     4501 tgcagggcgt cactccggag cacgtggtga tcgaggcact ggagcaggtt cccgtgcctc
     4561 tcgcggcggc gagctgaggc gaacgagttg gccgacacgg cgagagagtc ggtggatccg
     4621 gtggacgcgc
//
LOCUS       DAOSPL010000132         4626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_18644_length_4626_cov_3.190112, whole genome shotgun sequence.
ACCESSION   DAOSPL010000132 DAOSPL010000000
VERSION     DAOSPL010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18644_length_4626_cov_3.190112"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..485)
                     /locus_tag="PLQ54_03670"
     CDS             complement(147..485)
                     /locus_tag="PLQ54_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOS92385.1"
                     /translation="MAREIARIVRDRLPVEDLGRVVSVRVDVGEFSGVVPEALAFGFE
                     SVVADSAMRDARLEAVPVPVALRCRACSAGFRLAEPPFACPRCGASDVEVLGGVDVVV
                     RSIELKGEGP"
     gene            complement(507..830)
                     /locus_tag="PLQ54_03675"
     CDS             complement(507..830)
                     /locus_tag="PLQ54_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018670800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HOS92386.1"
                     /translation="MADENEAVEHPYGRVAAALRYDPDHDAAPRVAASGRGHVAERII
                     ELAREHGIPLREDRVLAAALAQLDLGELIPPQMYKAVAEVLAFVYRLDAERSRERSQS
                     LMRTE"
     gene            complement(846..3743)
                     /locus_tag="PLQ54_03680"
     CDS             complement(846..3743)
                     /locus_tag="PLQ54_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOS92387.1"
                     /translation="MNVLTTGPQVLRVDALAADLRRRGLGLSPGTRQVARVVSVSDGR
                     AVVNIGGETLVTQTDLPLREGQVVALLVEQGENGALLLRLTEPAQTIQRAGLRLAPEG
                     AISPRTDADIAEAVRAVGLPTTEDHMAAARALLDHGQALTKANISFVARNAHRFEGPT
                     EEVVAALAQARALDLPATEATIAALRAASRGTGLALAERLDGIARLASELQTALAELT
                     GAVPAAEPSGGALTTGAGELLAALSRALGLLRGSAVGADAADPRIGLAQRLSALLAAM
                     EFGGVVPIASQTLDRLASQLGQTPGKVIEESAPLLGRPLVQIAADIEESMGQLERFLT
                     DREPVTGLPSRLLAVLAAVDEALPTPAPAAAASGAPAAGSRERAEAALLPAAGEPADP
                     APPSAASELLSRLTTLRTALRAFSLPTTLSPAAADLGEMTDRAIQQLAGRVESTMVVV
                     AAEAPADVGALVTALAQRAAEVRVPSEAGIHPAVRLASLLEAVEDTLAALPADRASSS
                     DALLGLRHQLQGIAAFLRESRGSADVSLLAQRGAERVLEAVSSYAQATEERVAAVGEA
                     LAMRTHRDALIVVARVAAATARNAAQGLARVEGLLLDEGPAAPGGWITPGQLLALHDA
                     LDTATVRVAQALLGLGAITDPLSRIDPGLAQAAAALSASAEPASARSPEQLLQWLGRL
                     QPLLADPAMRAQLPTEVVRSARALAVAMALEATRSVAAESARVTRLLESAQTHPDRGT
                     PQVHEDLRRLVELVGRSLENKLLSTDAARAVERDPRAVLGRLEVALERLVRSPSTAEH
                     DPALAEQAQRLLSAVTAARSALEGQQLLNAVAPRTPLPHFAYVEVPVIVDGRQTQAQL
                     KVLRDSGRSGEPLDPDNLRVALRLDTYTLGTVTALVEMRQGILNVEFSLDSPESEAVV
                     DERLPELLSALQDGGLVVARLQTDVRQEGAADAFEALGEPVLAPFRISALA"
     gene            3773..>4626
                     /locus_tag="PLQ54_03685"
     CDS             3773..>4626
                     /locus_tag="PLQ54_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOS92388.1"
                     /translation="MRLPRPVRQTGYLLSLGAMLAGTTVLTLVTYGETDVATQFHYIP
                     IIISAFLFGTLGALICAVVAGFLAGPYLLITGRVSDPQWLPWITRFLFYAVIGLITSR
                     LSATIMQQAKEFQALFHVAQAINSSLRPSSVLRRIVRHARNRLRIRAVSIRLLDPKTG
                     ELALAASEGLSPSYIAKGRVDVDSSAIDRQALDGQTVVVEDVARNDQLQYADEVRSEG
                     IGAIMVVPLKSRRRPLGVFRAYASKPKRFRKAERSLIEAFANQAATAIENAELYDSLR
                     QSYFETVRS"
BASE COUNT          723 a         1694 c         1555 g          654 t
ORIGIN      
        1 gatggtggcc tcgagcaagg aggtcttgcc ggacccgggc gcgctgagta ggttcacggc
       61 gaggactcgc cgctcggcca gccgtctccg cgtctccgcg gcgatccggt cgttggtagc
      121 gaaggcactc tcctcgacgc tcaccctcaa ggcccctccc ccttcagctc gatggaacgg
      181 accaccacat ccacaccgcc gaggacctcg acatccgaag ctccgcatcg tgggcacgcg
      241 aaggggggct ccgcgaggcg gaagccagcc gagcacgccc tgcatcggag cgccaccggg
      301 acgggcactg cctcaagtcg cgcatcccgc atggccgagt ccgcgaccac cgactcgaac
      361 ccgaacgcca gagcctcggg caccacgcca gagaactcgc ccacgtcgac ccgcacgctc
      421 accacgcggc cgagatcctc caccggcaga cggtcgcgga cgatccgcgc gatctccctg
      481 gccagcgttg tctcgtgcag atcggatcac tccgtgcgca tcagggactg cgaacgctcg
      541 cggctgcgct cggcgtcgag ccggtagacg aaggcgagta cttcagcgac ggccttgtac
      601 atctggggcg gaatcagctc tcccagatcg agctgcgcca gggccgcggc cagcacccga
      661 tcctcacgaa gcgggatacc atgctcgcgg gccagctcga taatgcgctc ggccacatgg
      721 ccccgcccac tggccgccac gcgcggcgcc gcgtcgtggt ctgggtcata ccggagcgcg
      781 gccgccactc ggccgtaggg gtgttcgact gcctcgttct catcggccac gcgcgtcacc
      841 gctccctaag ccagcgccga tatccggaag ggggcgagca ccggctcgcc cagcgcctcg
      901 aaggcgtccg ccgcgccctc ttggcgcacg tcggtctgaa gccgcgccac caccagcccg
      961 ccatcctgaa gagcactcag gagctcgggc aggcgctcgt cgactacagc ctcggactcg
     1021 ggtgagtcca gggagaactc gacattgaga atgccctgcc gcatctcgac gagcgccgta
     1081 acggtaccca acgtgtaggt gtcgagccgg agagccaccc tcagattgtc cgggtcgagc
     1141 ggctctccag accgtcccga gtcccgcagg accttgagct gagcctgagt ctgtcgcccg
     1201 tccacaatca cgggtacctc gacataggcg aaatggggca acggggtccg aggagccacc
     1261 gcgttcagga gctgctgccc ctccagcgcc gatcgcgccg ccgtgaccgc cgagaggagt
     1321 cgctgagcct gctcggccag cgccggatcg tgctccgccg tcgatggcga ccgcaccagg
     1381 cgctcgagcg caacctcgag ccgcccgagc accgcgcgcg ggtcgcgctc gacggcgcgc
     1441 gccgcgtcgg tggacagcag cttgttctca agactgcggc ccacgagctc caccaggcgt
     1501 ctcaggtcct cgtgcacctg cggcgtaccg cgatccgggt gagtctgagc ggactcgagc
     1561 aggcgcgtca cgcgcgccga ttccgcggca acgctgcggg tcgcttccag ggccatggcg
     1621 acggccagcg cccgcgccga ccgaaccacc tcggtgggca gctgggcccg catggccgga
     1681 tcggcgagca ggggctggag ccgccccagc cactggagga gctgctcggg acttcgggcc
     1741 gatgccggtt cggcacttgc cgacagggcc gccgctgcct gggccagacc cgggtcgatc
     1801 cggctcagcg gatcggtgat tgccccgagg ccaagcagtg cctgggcgac tcgtaccgtc
     1861 gccgtgtcca gcgcgtcgtg gagggcgagc agctggcccg gtgtgatcca gcctccgggc
     1921 gcggccgggc cctcatcgag aagcaggccc tcgacgcgcg ccaagccctg ggccgcattg
     1981 cgagccgtcg cggccgccac acgagcgacg acgatgagcg cgtcgcgatg ggtccgcatg
     2041 gccagcgcct cgccgacggc cgcgacccgc tcctcggtgg cctgcgcata cgacgagacg
     2101 gcttccagca cgcgctcggc accccgctgg gccagcaagg agacatccgc cgacccgcgg
     2161 gactcccgta ggaacgccgc aatgccctgg agttgatgac gcagacccag caacgcatcg
     2221 gaactcgacg cgcggtcggc ggggagagcg gccagcgtgt cctcgacggc ttcgaggaga
     2281 ctggcgaggc gcaccgccgg atggatgccg gcctcggagg gcacccgtac ctccgcggcc
     2341 cgctgggcca gcgcggtcac cagcgccccg acatcggccg gggcctccgc agccacaacc
     2401 accatggtgg actcgactcg gccggcgagc tgctgtatgg cgcggtccgt catctcccca
     2461 aggtcagcgg cggccgggga gagcgtggtc ggaagactga aggctcggag ggcggtccgc
     2521 aaggtggtga gtcgcgagag cagctccgag gcggccgagg gtggcgctgg atcggcaggc
     2581 tccccggcgg ctggaagcag cgccgcctcc gcgcgttccc ggctgccggc agcaggcgcg
     2641 ccgctggccg cagccgccgg cgcgggcgtg ggcagagcct cgtcgaccgc ggccagaacg
     2701 gccagcagac ggctcggcag gcctgtcacg ggctccctgt ccgtgagaaa cctctcgagt
     2761 tgccccatcg actcctcaat atccgcggcg atctgcacta atgggcggcc aagcagcggc
     2821 gcagactcct cgataacctt gcccggcgtc tggcccagct gggacgccaa gcggtcgagg
     2881 gtctgactgg cgatgggcac cacgcccccg aactccatcg ccgccaacag ggccgacagg
     2941 cgctgggcca gcccgatacg tggatctgcg gcgtcggcgc ccactgcgct gccccgcaga
     3001 agacccaggg cccggctgag cgcggcgagg agctcgcccg ccccggtggt gagagccccc
     3061 cccgacggct cggccgccgg gacggccccc gtgagctccg ccagagccgt ctgcagctcg
     3121 ctggctagcc gcgcgatgcc atccaggcgt tccgcgaggg ccaggcccgt gccccgagaa
     3181 gccgccctca gcgccgcgat tgtggcctcc gtggccggca ggtcgagagc ccgggcctgc
     3241 gccagggccg cgacgacctc ctcggtgggt ccctcgaatc ggtgagcgtt gcgggccacg
     3301 aaactgatgt tcgccttggt cagggcctgg ccgtggtcca gaagggcgcg tgccgcggcc
     3361 atgtggtcct cggtggtggg cagacccacc gcccggacgg cctcggcgat gtcggcatcc
     3421 gtccgaggcg atatggcgcc ctccggggcc agccgcaacc ccgccctctg gatggtttgg
     3481 gcgggctcag tcagtcggag gaggagcgcg ccattctctc cctgctcgac gaggagagcc
     3541 accacctgcc cctcgcgcag gggcaggtcc gtctgtgtta ccagagtctc accgccaatg
     3601 ttgacaacgg ctcgcccatc gctcacactc acgacgcgcg cgacctgtcg ggtgccgggg
     3661 gacagcccaa ggccccgccg acgcaggtcc gccgccagag catcgacccg cagcacttgg
     3721 ggccccgtgg tgaggacgtt catgtaccgg ctcccctgca gggaggacta aggtgaggct
     3781 cccgaggccc gtgcgtcaga ccgggtacct cctgtccctc ggcgccatgc tcgcgggcac
     3841 gacggtgctc accctcgtga cgtatggcga gacggatgtc gcgactcagt tccattacat
     3901 ccccatcata atatcggcct tcttgttcgg cacccttggg gcgctgatct gcgcggtagt
     3961 cgccggattc ctggcggggc cctacttact gatcaccggc cgggtcagcg acccccagtg
     4021 gctgccctgg atcacccgct tcctgttcta cgccgtcatc ggcctgatca cctcacggct
     4081 ctcggccacc atcatgcagc aggccaagga gttccaggcg ctgttccatg tagcccaggc
     4141 catcaactcc tcactccggc cgtccagcgt gctccgacgc atcgtgcgac acgcccgaaa
     4201 ccggctgcgc atccgagcgg tctctatccg cctgctcgat cccaagacgg gggagctggc
     4261 tctggcggcc agcgagggcc tctcgccctc gtacatcgcc aagggcaggg tggacgtcga
     4321 cagcagcgcc attgatcgcc aggctctcga tggccagacg gtggtggtcg aagacgtcgc
     4381 tcgcaacgat cagctccagt atgccgatga ggtgcgcagc gagggtatcg gggccatcat
     4441 ggtcgtgccg ctgaagtcca ggcgccggcc tctgggcgtc ttccgcgcct atgcgtcgaa
     4501 gcccaagagg tttcgcaaag ccgagcgatc gctcatcgag gccttcgcca accaagccgc
     4561 caccgccatc gagaatgccg agctctacga cagcctgcgc cagagctact ttgagacggt
     4621 ccgatc
//
LOCUS       DAOSPL010000133         4620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_18679_length_4620_cov_2.717196, whole genome shotgun sequence.
ACCESSION   DAOSPL010000133 DAOSPL010000000
VERSION     DAOSPL010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18679_length_4620_cov_2.717196"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..2791)
                     /locus_tag="PLQ54_03690"
     CDS             complement(200..2791)
                     /locus_tag="PLQ54_03690"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HOS92389.1"
                     /translation="MNTRRSALAALALVGALAAPVSAQGVLIGRDTDRAADGPSAPLA
                     IRSYAADVVIKDTVAKTRIDQVFVNSTDRPLEGTFYYPLPQGAVVSEFTMYIGSTPVK
                     GELVEQTQARGIYNDIVRRTRDPAILEYAGRNLLKMDVFPIEAQSDKRIILEYAQQLP
                     IEGDMVRFVHPLDAGKMVKYPVGSLSIACSIESSHGIKAIYSPSHEIDTFREGDHKLT
                     AGLEASNVVTLKDFTLFYTLSDADFGLSVLSQRPKGDEDGYFMLLLAPKQEFAQEAIQ
                     AKTVVFVFDTSGSMNGEKIEQARNALTYCLGNLGKDDHFNVVTFSTAVSVLSPTVLPA
                     TPDEIAKAKEFVGKIAARGGTNIEEALKTALASIDGADGPTMVVFMTDGLPTVGVRDI
                     DEILKSVAAANTAEGGTPKSRVFVFGVGDDVNTHLLDKLASSSGAASQYVLPGEDIEV
                     AVSTFYEKVSHPVMTDVRLDFGDVYVHDYYPQDLPDLFKGSQLTVFGRYRGAGEVAVK
                     LRGDVGGKQQEYAYDARFAEIEEANDFVPRLWATRRVGHLLDEIRLHGETQELKDEVI
                     ALGKEWGLVTPYTSYLVLDEAERARYRNNTAWGVAPSPASTGASPPAVTWMDRDRTAA
                     GAMMGGPMMGPGGMPGMGGGGFGGMMGGGMGVPPIPGPAGPAGPKGDRGASGPAGPAA
                     GDVGGAEGLRFGETDIVADRLAEPEPAFSLHYGTAARQQTGRAAVELAQALGKAKQVE
                     VEGGATASQRAGGRTFYYIDGLWVDSALQEGAEITLVRCLSDAYFALAATDWVAQCLA
                     LGERVVIVLPSGKVIQTVTEEDAAQLNAAPPVAEGQVATAPAKVIETLTADEVAALMK
                     PAEAPAK"
     gene            complement(2955..3329)
                     /locus_tag="PLQ54_03695"
     CDS             complement(2955..3329)
                     /locus_tag="PLQ54_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92390.1"
                     /translation="MGPRAAGRTGQRLDDVFTPAAFRVDRRRVIGRADGVGAGRHHCR
                     CRGGLTANEFTPAPPIDDEGGGLLRDPVADNEGLLPDNVRACINFDGNGCADYPPPRN
                     HRDSEGQCGQEGVPPPSDPTGG"
     gene            3685..>4620
                     /locus_tag="PLQ54_03700"
     CDS             3685..>4620
                     /locus_tag="PLQ54_03700"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HOS92391.1"
                     /translation="MGASGILLDLVHNIALLVSLILGYQMIVRRVRQQGLRLQVVSGL
                     LFAGVGLIGMMTPVRFIPGIIFDGRSIVLSNAGLFGGPVAAAIAGSICAAYRLHLGGG
                     GALMGVSVIAESCLIGVAFHYLRRRHPAVVHPLGLLGIGVLVHVVMFLMTMLLPGGAG
                     GDAMRRLGFAILVAYPVGSMLVGMLFVGQEGNLRAEQALRESEERFRSMAEQLTDLLF
                     VTSPHGVITYASPASEHLVGYTPEEMTGRRLADFAVEDDRDAVEAALGGVARGGQRCE
                     GLALRLCHRAGSVLTAEMVASPSRLGAERGVLASSV"
BASE COUNT          675 a         1611 c         1529 g          805 t
ORIGIN      
        1 cctctccctc tgcgcaccgt cacaagtgtc ctgagccggt agaaccggct gagttcactt
       61 gttcgggagg cgaggggtat cgacctgcag gcggcgcggg agctcggggc agcgctggcc
      121 cctgcagagt acgggggcgg gtggaggccc tcgcagaact ccgaggcctg tgacccgccc
      181 ccggctctcg gcggggctgc tacttcgccg gcgcctcggc gggcttcatc agcgccgcga
      241 cctcgtcggc cgtgagggtc tcgatcacct tcgccggcgc ggtcgccacc tggccctcgg
      301 ccaccggggg cgccgcattg agttgcgcgg catcctcctc ggtcaccgtc tggatcacct
      361 tccccgacgg caggacgatg acgacccgct cgcccagcgc caggcactgg gcgacccagt
      421 ccgtagcggc cagcgcgaag taggcgtcgc taagacaccg caccagcgtg atctccgcgc
      481 cctcctgcag cgcggaatcc acccacaggc cgtcgatgta gtagaaggtt cggccgcctg
      541 cacgctggga cgcggtggcg cccccttcca cctcaacctg cttggccttt cccagggcct
      601 gcgcgagttc gacggccgcc cggccggtct gctgcctggc ggcggttccg tagtgtaggc
      661 tgaaagctgg ctcaggctca gccaagcggt cggccacgat gtccgtctca ccgaaccgga
      721 gcccctcggc tccgcccacg tcgccagccg ccggtcccgc aggaccgctt gcgccccggt
      781 cgcctttggg accggcaggg cccgcgggcc ccgggatcgg cggcacgccc atgccgccgc
      841 ccatcatgcc gccaaagccg ccaccgccca ttccaggcat gccacccggg cccatcatgg
      901 ggccacccat cattgcgccc gctgcggtgc ggtcgcgatc catccaagtc acggcggggg
      961 gcgaagcccc ggtgctggcc gggctcggtg ccacgcccca cgccgtgttg ttccggtacc
     1021 gcgccctctc ggcctcatcg agcacgagat aggaggtata cggggtcacc agaccccatt
     1081 ccttgcccag ggcgatgacc tcgtccttca gctcctgcgt ctcgccatgg agccggatct
     1141 cgtccagcaa atgcccgacg cggcgcgtgg cccacaggcg tggtacgaag tcgttggcct
     1201 cctcgatctc ggcgaaccgc gcgtcatacg cgtactcctg ctgcttcccg cccacgtcgc
     1261 cccgcagttt cacggcaacc tctccggcac cgcggtagcg cccgaacacg gtgagctgcg
     1321 agcccttgaa caggtcgggc aggtcctgcg ggtagtagtc gtgcacgtac acgtcgccga
     1381 agtccagccg cacgtccgtc atgaccggat ggctgacctt ctcatagaag gtcgacacag
     1441 cgacctcgat gtcctcgccg gggagcacgt actgcgaggc cgctcccgac gacgatgcca
     1501 gtttgtccag gagatgcgtg ttcacgtcgt cccccacgcc gaacacgaac acccgcgact
     1561 tgggcgtgcc accctctgct gtgttggccg ctgcgacgct cttgaggatc tcgtcgatgt
     1621 cgcgaacgcc cacggtgggt aagccgtcgg tcatgaagac gaccatggtg gggccgtccg
     1681 caccgtcgat agacgcgagc gctgttttca gggcctcctc aatgttcgtg ccgccgcgag
     1741 ccgcgatctt gccgacgaac tccttggcct tggcgatctc gtcgggcgtg gccggcagca
     1801 ccgtcggcga gagcacgctc accgccgtcg agaaggtgac gacgttgaag tgatcgtcct
     1861 tccccaggtt gcccaggcag tacgtgagcg cgttccgcgc ctgctcgatc ttctcgccgt
     1921 tcatcgagcc cgatgtgtcg aacacgaaca ccacggtctt cgcctggatg gcctcctggg
     1981 caaactcctg tttcggcgcc agcaggagca tgaagtagcc gtcctcatcg cccttgggcc
     2041 tctgcgacag gacgctcagg ccgaagtcgg catccgagag cgtgtagaac agagtgaagt
     2101 ccttgagcgt caccacgttc gaggcctcaa ggccggccgt gagtttgtgg tcgccctccc
     2161 ggaatgtgtc aatctcgtgc gaggggctgt agatggcctt gatgccatgg gagctctcga
     2221 tggagcacgc gatggacagg gacccgaccg ggtacttcac catcttgcca gcatcgagcg
     2281 gatggacgaa ccgcaccata tcgccctcga tgggtagctg ctgagcatac tcgaggatga
     2341 tgcgcttgtc gctctgcgcc tcgatgggga agacatccat cttcagcagg ttgcggcccg
     2401 catactcgag gatggccgga tcgcgggtcc ggcgcacgat gtcgttgtag atgccccgag
     2461 cctgggtctg ctcgaccagt tcgcccttca ccggcgtgct gccgatgtac atggtgaact
     2521 cggacaccac cgctccttgg ggtagtgggt agtagaacgt gccctcgagt ggccggtcgg
     2581 tgctgttgac gaagacctgg tcaatcctgg tctttgccac cgtgtccttg atgaccacgt
     2641 cggcggcgta ggagcgaatg gcgaggggcg cgctgggccc gtcggcggcg cggtccgtgt
     2701 cgcgtccgat aagcacgccc tgcgcggaga cgggcgctgc cagcgcgccc accagggcca
     2761 gcgcagctag tgcggatcgt cgggtgttca cctgggttca cctcgagacg gggaatgggt
     2821 cgatctcgat cgtagagtag gaccccgcag aggcccgctg tgttccagcg aactccgggg
     2881 ctcaggggcg tgcgccgtgt caactcgctt cgcacgcacc cgcggaccag ccaccccaat
     2941 gcagcagcct cggctcaccc tcccgttggg tcactcggag gcggcacacc ctcctgcccg
     3001 cactggccct cgctgtccct gtggttgcgc ggtggcgggt agtcggcgca gccgttcccg
     3061 tcgaagttga tgcatgcccg gacgttgtcc ggcagtaacc cctcgttgtc ggcgacaggg
     3121 tctcttagca gcccgccccc ctcgtcgtcg atgggcggtg ccggcgtgaa ctcgttcgcc
     3181 gtgagccccc cacggcatcg acaatgatgc cgcccggcgc cgacaccgtc agcccgccca
     3241 atgacacggc gccggtcgac gcgaaacgca gcgggtgtga agacgtcgtc cagacgctgc
     3301 ccggtccgac cagccgcccg cgggcccaac accgtcgccg gtggtgggcg gtggtccccc
     3361 acagccggcg gcgaggtgaa tcagcgccgc acataggccg agcagcggcg ccgggcgtgc
     3421 cggacgtcct gtccgggttc gcgcgggtct gtgaggcatc acacatccgt gcttcgcgtt
     3481 cgcggcgggt gaggtgatcc gagtcgtcga ttcgcgcggc gaagcgtgag tccatcgggc
     3541 gaatcccgcc accgctgcga ctcccgcgcc agaaccccca cgacgccgag gagtccacgc
     3601 accgatgagc gaaactgtca ggcggaaggt gcgccgagcg cccggcatca gcgacgggcg
     3661 gcgccaccag gtgccgtacc tgaggtgggg gcgagcggga tactcctcga tctggttcac
     3721 aatatcgcgc ttctcgtttc gcttatcctc gggtaccaga tgatcgttcg ccgcgtccgc
     3781 cagcaggggc ttcggctcca ggtggtgtcc ggactcctgt tcgccggcgt cggcctcatt
     3841 gggatgatga cccctgtccg cttcatcccc gggatcatct tcgacggccg atccatcgtg
     3901 ctcagcaacg ccggcctgtt cggaggccct gtcgcggccg ctatagccgg atccatctgc
     3961 gccgcctacc ggctgcacct gggcggcggc ggagccctca tgggtgtcag cgtcattgcc
     4021 gagtcctgcc tcatcggcgt ggcattccac tatctgcgga gacgtcaccc tgcggtggtc
     4081 caccccttgg ggctgttggg tatcggggtc ctggtgcacg tcgtgatgtt cctcatgacc
     4141 atgctcctcc ccggcggggc cggcggcgat gccatgcgac gcctcggctt cgcgatcctc
     4201 gtcgcctatc ccgtcggcag catgctggtg ggaatgctgt tcgtggggca ggaagggaac
     4261 ctccgggctg agcaggcact gcgggagagc gaggagcggt tccgctccat ggccgagcag
     4321 ctgacggatc ttctgttcgt caccagcccc catggcgtga tcacctatgc ctcgcccgca
     4381 tccgagcacc tggtggggta cacaccggag gagatgaccg ggcgccggct ggctgacttc
     4441 gccgtcgagg acgaccggga cgccgtcgag gcggctctgg gcggcgtcgc ccgggggggc
     4501 caacgctgcg agggactggc cctgcgcctc tgtcaccgtg ctgggtctgt cctcaccgcc
     4561 gagatggtcg ctagcccgtc ccgactcggc gcggaacggg gggtgctcgc gtcatccgtg
//
LOCUS       DAOSPL010000134         4613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_18717_length_4613_cov_3.195480, whole genome shotgun sequence.
ACCESSION   DAOSPL010000134 DAOSPL010000000
VERSION     DAOSPL010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18717_length_4613_cov_3.195480"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            353..586
                     /locus_tag="PLQ54_03705"
     CDS             353..586
                     /locus_tag="PLQ54_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92392.1"
                     /translation="MSLELIEHSRQKRFEHQCPGRVFAEQERHAPKAAGTSGSRRRIR
                     PIPERTSHLHDAPASLGIVTLTLAPDDAAHSLP"
     gene            789..2780
                     /locus_tag="PLQ54_03710"
     CDS             789..2780
                     /locus_tag="PLQ54_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92393.1"
                     /translation="MGPRSSLPGPLVWLALNVAVSISAVAQGGTTEPYTDARHLTAIP
                     FGGHSHWAQPWRSYIETVPAHRFVDGIGVGFTAPEGASEDLIAEMLARHGVRHVRIEI
                     GWGNLNWDDQLPPEARDRLAKRIRACASRGLRPLILLNAHHGAPCPARFFERVLAAPA
                     RRGDTTVRLTDTADLALGFSGLSNLTDYWAAEALITAIDGDTVTLSKPLPKDLGEAGA
                     SVPMATLKYRPFGPPDSADTAATTEAWQRYAGEVAGFVAEALATAGQSDLGFDMEIWN
                     ELSFGSNFTQLNAYYEPKAYDYNGDVVWGNLVRATAEYVDQHPAQFAGVRLCDGFSNT
                     IPWTASSQEPARVTALSKHPYAGRKTYPQDEQRGDAYNAVFERDAAPGFVPTYSVSFP
                     EYYATFLQTETLFRDLAPITTDIYGTKHGRLAREIDGKVAPCEVWFTEVGFAPNENGV
                     TDPERALALKAKTTARYYAFYLGKGLERLYLYAACEGDLWLGVVGDRFVGYAKTHTEY
                     PEDDWSYASPALKVIQRMSDTLSDGMDRDLAATRPIHVESVTDQHGATVFEGDGTPQH
                     PSLYHRDVLAVLPYQVNAHRFVLAYYVMTRDVLEDLPAEEFDVSLTGIRADGAKLRAH
                     DPITGATVPASAVASGADGLTLRLSATDYPRLLIIDEVE"
     gene            2796..3050
                     /locus_tag="PLQ54_03715"
     CDS             2796..3050
                     /locus_tag="PLQ54_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92394.1"
                     /translation="MPSAIQRKALIHEAFGLCVLLPKTHWRRAPEPGGSARVLLNRAE
                     VEVTVRPEECDCRGTGAHEHRFLALPAGVAGPGETLTVEV"
     gene            3084..4247
                     /locus_tag="PLQ54_03720"
     CDS             3084..4247
                     /locus_tag="PLQ54_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92395.1"
                     /translation="MRRSTTLSAVAVLAVALLAAPRAVGAEVALASVHEELDPFLTTP
                     AAGHLWEMPVLVLSYIPTADGVHVDPATTGWGSAEAPVPLADLRRRIMALTVRTKFAL
                     EEGSRFRAYSDPAAKPSLGYRIVGMVTVFEPVPPGQERPWGDPGCHFPDYNQILTRAG
                     IAHFVEDLGVKEVWLWMYHTAEREPVESNMSSPVTGDISNSSRFADDLPVLTKTYIVY
                     SYNFTRDARCALENHMHQLESQLSHVNTLRDGNNDLFDRDFVGSGSRKTLGRCGWTHC
                     PPNTLADYDVASEVEVESDIEDWTPDGSGHKKRINCRAWLDKTYAWPDGKSRFDEWEG
                     TYWFIYWMQSHPGRGNRIRYGGRWMTNWWALVGDWDGCIQSGFGLHGEQADRG"
     gene            complement(4418..>4613)
                     /locus_tag="PLQ54_03725"
     CDS             complement(4418..>4613)
                     /locus_tag="PLQ54_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92396.1"
                     /translation="QAGGPRDLRETQPPGPGPYVTANACLRPLPGYGRRFEGPLTGNQ
                     VVLAMRAVPDAARIALGSTK"
BASE COUNT          762 a         1562 c         1552 g          737 t
ORIGIN      
        1 aagctcagga agagacgcac ccgaatggtc atcgcccaat ctccactccc tcggtcgctg
       61 tggtgacgca tcgtgcccaa cgatctcgcg cggttgcgct caggtggacc gtcgcggccc
      121 ctgccatgcg tggcggtgcc gggtgttggg acgggcgggt cgagctggtc ggaaccggcg
      181 cgctcaaccc agccccggtc gccagggaca tacactggcc aagggtcggt ggcagcagag
      241 gacgtagcgc ggaacagcgc ggggacgcac cgcgctcccg caggatccgc gggacatagg
      301 ccgcatcggt gactcgagcg tcaggcagac gcgagacgag gagctgcagg tcttgagcct
      361 cgagctcatc gagcactccc gccagaagcg gttcgaacac cagtgccctg gccgggtctt
      421 cgctgagcag gagcgacacg cacccaaagc ggccggaacg agtggctcgc gccgacgcat
      481 taggccgata cccgagcgca cgagccacct ccatgacgcg ccggcaagtc tcggcatcgt
      541 gacgctcaca ctggccccgg acgatgcggc tcacagtttg ccgtgatacg ccacactgct
      601 cggcgatgtg ccggagcgac acccggtcgg ggcggcacct tgcgcgctgt ggactggtgt
      661 tcggtgtttc catgttatcg atcacactag cgggcagcat cgccagcgtc aatccttccg
      721 tcgtgcccca acttgcctcg ccgtttcgcc tcgcgtagtg taccgcccac catggaggtg
      781 gtctgctgat gggtccgaga tcgtctcttc caggtccact ggtctggctc gctctgaacg
      841 tcgcggtctc catttcggcc gtcgcccagg gcggcaccac cgaaccctac accgatgccc
      901 ggcatctcac cgcgatcccc ttcggcgggc actcgcactg ggcccaacct tggcggtcct
      961 acatcgagac cgtgcccgcg caccggttcg tcgatggcat cggcgtaggc ttcacggcac
     1021 ccgaaggtgc gtcggaagac ctgatcgccg agatgctcgc ccggcatggc gtgcgccatg
     1081 tgcggatcga gatcggctgg gggaacctga actgggacga ccagctccct ccggaggcgc
     1141 gcgaccggct ggcgaagcgc atccgcgcgt gtgcctcgcg cgggctgcgg ccgctgatcc
     1201 tgctcaacgc ccaccacggg gctccttgcc cggcgcggtt cttcgagcgc gtgttggccg
     1261 ctccggcgag gagaggcgac accaccgtcc gcctcaccga cacggcggac ttggcgctcg
     1321 gcttctcggg cctcagcaac ctgaccgact actgggcggc cgaggcgctc atcacggcga
     1381 tcgacgggga tacggtcacg ctctcgaagc cgctgcccaa ggatttgggc gaagcgggag
     1441 cgtcggtacc catggcgaca ctcaagtacc gcccattcgg cccgccagac tcggccgata
     1501 ccgcggcgac gactgaggcc tggcagcgct acgcgggcga ggtagctggc tttgtggccg
     1561 aggcgctcgc caccgcgggg cagtctgatc tcggcttcga catggagatc tggaacgagc
     1621 tgtcgtttgg ctcgaacttc acccagctca acgcctatta cgagccgaag gcgtacgact
     1681 acaatggcga cgtggtctgg ggcaacctgg tacgggcgac ggccgagtac gtcgaccagc
     1741 accccgcgca gttcgccggc gtccggctgt gcgacggttt ctcgaacacc attccgtgga
     1801 cggcttcctc gcaggagccc gcgcgcgtga cggcactcag caagcacccc tatgccggcc
     1861 ggaagaccta cccgcaggac gagcagcggg gtgatgccta caacgccgtg ttcgaacgag
     1921 acgccgcacc cggcttcgtc ccgacctact cggtctcgtt ccccgagtac tacgcgacct
     1981 tcctgcagac cgagacgctg ttccgcgacc tggcaccgat tacgaccgac atctacggca
     2041 cgaagcacgg ccggctggcg cgggagatcg acggcaaggt ggcgccctgc gaggtgtggt
     2101 tcaccgaagt agggttcgcg ccaaacgaga acggcgtcac cgaccccgag cgggcgctgg
     2161 cgcttaaggc gaagaccacg gcgcggtact atgccttcta cctcggcaag gggctcgagc
     2221 ggctctacct gtacgcggcg tgcgagggtg atctgtggct cggggtcgtt ggcgaccgct
     2281 ttgtcgggta cgccaagacg cacaccgagt atccggagga cgattggtcc tatgcgtcgc
     2341 ccgcgctgaa ggttatccag cgaatgtccg acacgctctc agatggcatg gatcgcgacc
     2401 tcgccgcgac ccgcccgatc cacgtcgagt ccgtgaccga ccagcacggc gcgacggtgt
     2461 tcgaaggtga cggcacgcct cagcacccgt cgctctacca ccgtgacgtg ttggctgttc
     2521 tgccctacca ggtgaacgca catcggttcg tcctagccta ctatgtgatg acgcgcgatg
     2581 tgctcgagga cctgccggcg gaggagttcg atgtgagcct cacgggcatc cgggcggacg
     2641 gcgcgaagct gcgcgcccac gatcccatca ccggcgcgac cgtgccggcg agtgccgtcg
     2701 ccagtggcgc agatggccta acgctgcgcc tcagcgcgac ggactaccct cgcctgctga
     2761 tcatcgacga ggtggagtga cggagggagt agcccatgcc atcggcgatc caacgcaagg
     2821 cgctcatcca cgaagccttc gggctgtgcg tgctgctgcc gaagacacac tggcggcgcg
     2881 cgccagagcc cggtgggtcg gcgagggtgc tgctcaaccg cgcggaggtc gaggtcaccg
     2941 tccgcccgga agagtgcgac tgccggggca cgggcgcgca cgagcaccgg ttccttgcgt
     3001 tgccggctgg cgttgccggc cccggtgaga cgctgaccgt ggaggtgtaa cggcagcgcg
     3061 gtgacgctga tcgggggctg agtatgcgac gatcgacaac gctgagcgcg gtggccgttc
     3121 tggccgtcgc cctcctggcg gcgccccgcg cagtgggcgc cgaggtggct ctggcgtccg
     3181 tccacgagga gttggaccca ttcctcacca cgccggctgc cggccacctg tgggagatgc
     3241 cggttctggt gctgtcctac atcccgacgg ccgacggcgt ccacgtcgac ccggcgacta
     3301 ccggctgggg ctcggccgag gcccccgtcc ccctggccga tctgcgcagg cggatcatgg
     3361 cactgacggt ccgcaccaag ttcgcgctcg aggagggcag ccgcttccgg gcttactccg
     3421 acccggcggc caagccgtca cttgggtacc gcatcgttgg aatggtgacg gtgttcgagc
     3481 cggtcccacc gggccaggag cggccctggg gcgatcccgg ctgtcacttc cccgactaca
     3541 accagatcct gacgcgcgcc ggcatcgccc acttcgtcga ggacctgggc gtgaaggaag
     3601 tctggctgtg gatgtaccac actgccgagc gcgagccagt cgagtcgaac atgtccagcc
     3661 cggtcacggg cgacatctcg aacagctcgc ggttcgcgga tgatctgccg gtgctcacca
     3721 agacgtacat cgtctacagc tacaacttca ctcgcgatgc ccgttgtgcg ctcgagaacc
     3781 acatgcacca gctcgagagc cagctcagtc atgtgaacac gctgcgcgat gggaacaacg
     3841 acctgttcga ccgcgacttc gttggctccg gcagccggaa gaccttgggc cgctgcggat
     3901 ggacccactg ccccccgaac accttagccg actacgatgt ggccagcgag gtcgaggtcg
     3961 agagcgacat cgaggactgg acgcccgatg gcagcgggca caagaagcgc atcaactgcc
     4021 gcgcctggct cgacaagacc tacgcctggc cggacggcaa gagccgcttc gacgagtggg
     4081 agggcaccta ttggttcatc tactggatgc agagccaccc gggccgcggc aaccgcatcc
     4141 ggtacggtgg ccgctggatg accaactggt gggccctggt gggcgactgg gatggctgca
     4201 tccagtcggg cttcgggctg cacggggagc aggccgatag ggggtagggg caggtggctg
     4261 gtcgtcggcc cggagcgccc ttactcccct gcccccagcc ccctgaactg cacgttcagg
     4321 ccgtcgatgg cgcggcggtg gggctggtcg gtgtcggagg cgacgagcca actccccccc
     4381 tcgggatact gggcgcggaa cgacgtgagc ccactgttca tttggtgcta ccaagggcaa
     4441 tcctcgctgc atccggtaca gcgcgcatgg ccaataccac ctggttcccc gtcagtggcc
     4501 cctcaaaccg ccttccatag ccggggaggg gccgaaggca ggcattggcg gtaacatagg
     4561 gccctgggcc gggcggctgg gtctcgcgaa ggtcgcgcgg accgcccgcc tgg
//
LOCUS       DAOSPL010000135         4612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_18725_length_4612_cov_3.674347, whole genome shotgun sequence.
ACCESSION   DAOSPL010000135 DAOSPL010000000
VERSION     DAOSPL010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18725_length_4612_cov_3.674347"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2466)
                     /locus_tag="PLQ54_03730"
     CDS             complement(<1..2466)
                     /locus_tag="PLQ54_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92397.1"
                     /translation="MTRALGNLLALAALTAVAPASEPPVSLFVSPDGRDTWTGRLAAP
                     NPARTDGPLATLAGARDRLRALRANRRLGPVTVLVRGGTYVLSEPLALSPEDSGSEAA
                     PVVYQAFPNEHPVISGGRRIAGWHRAGGGLWMAKVPRGWYFTRLFVNGEKQTRSREPD
                     TDVWAQWFRVTRGGEPEPDAPEGMGSRQFYFPPGTIPRCENTLDVEINSLPSFRYANF
                     ISSLESVDEAHSLAVLKSMAYYNFAPGDPFRVENTLEAIDEPGEWCLSSERGTVYYLP
                     LSGVDMKAAEVVAPALKQLIRLVGSDRPADTVHDVAFRGLTFRHGDRSRWHEHPAEDE
                     ANLHALDSAVYLAGAARCAVESCLFEDVGGFAARFDRWCQDCRFVGNEVVGAGCGGLQ
                     AGGYGPGTLDVNHGHQISRNHIHHSSTDFWHAGAVDVRQSGHNHIACNWVHDMPYTGI
                     CISGAHTSYFNQYRERGEGVGRAKYNFRWDEIPDDNPLTPESVKPFLHGRDNVVEHNV
                     VHDVLWQLPADGGAIYGFGQGLGNVFRDNLVYRAHCLAIYLDAEFDDVLVQGNVVIDS
                     ASPFGGSGAHPRLVDNYFPAPGQATPEIRALARQMIAAIAQAAGPDWRRPGGAAVATE
                     PSVFSGEFGGLRPGPLPGQGAWEWFGPGPGIGVEDDGGVTVAVAGGTDSWAAARHGAV
                     LSPTQGFTLELEARITDNPGPNSSFELYLNAGGIHSDAAFGPAIVAGAQDGTPTALGV
                     RQDGAGPRILSGARLTLGHWYRVRLVVAADSTACRLYLRDLTALAQEVDVTPAGGVAL
                     ARAGVWSPPLEALDTLVLRLGDSA"
     gene            2861..4003
                     /locus_tag="PLQ54_03735"
     CDS             2861..4003
                     /locus_tag="PLQ54_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS92398.1"
                     /translation="MVDFAAHNEDVREVWRRYHEGNPSRVPMIIGTSVRFTLLDRSIN
                     ETGADFERFFNDPDVMFERHLFHQWWIRHHLPQDAEMGLPEAWTVSVDFQNSYEAMWF
                     GAPIRYFADDVPDCPPIVTDENKWTFIDHGPPEPFSGWMGRAWEYWEYFREKARSYEY
                     RGRPVHVGGVPGCGTDGPFTTACALRGATEICLDMYADPDFYHALMTLIVDATIGRMR
                     AFRKRLGVPVDSTVWGFADDSIQLLSTRTYEELVLPYHERLTAEFGAEGPNSVHLCGD
                     ATRHFRVIRDRLKVNSFDTGFPVDFGALRADLGPDVQIYGGPPVALLLSGTPQAIRQE
                     VRRILGTGVTEGGRFVLREANNLAPGTPMANVAAMYDACKEFGRYE"
     gene            complement(4085..>4612)
                     /locus_tag="PLQ54_03740"
     CDS             complement(4085..>4612)
                     /locus_tag="PLQ54_03740"
                     /inference="COORDINATES: protein motif:HMM:NF037628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92399.1"
                     /translation="LELLDRFRQEINAYARQTMPDELLRPVRTVDGELNPEDVSLRLC
                     NELARLAPFGTGNPEPVFLCRQARIELLKRVGDGSHLKLRVSGPTSSMLVEGIAFSMG
                     DLAEEFHVGDTVEMCFSAQANEWNGTVLPQMLVRGIRPAARQASDTDRSRRSEYHDRH
                     DEYPRFIESGGSRRE"
BASE COUNT          741 a         1470 c         1661 g          740 t
ORIGIN      
        1 cgcgctatcg ccgaggcgga ggacgagggt gtccagggcc tcgaggggag gagaccacac
       61 tcccgcccgg gccagagcca caccgccggc gggtgtgacg tcgacctcct gagcgagtgc
      121 agtcaggtca cgaagataca gcctgcaggc cgttgagtcg gcggccacga cgaggcggac
      181 gcggtaccag tggccgaggg tcagccgggc gcccgagagg atgcggggcc cggcgccgtc
      241 ctgtcggact ccgagggccg tgggggttcc gtcctgcgcg ccggccacga tggccggacc
      301 gaaagcagcg tcggagtgaa tcccgcccgc gttgaggtag agctcgaagg acgagttggg
      361 cccgggattg tcggtaatgc gcgcctcgag ctccagcgtg aagccctggg tgggcgagag
      421 gacggcaccg tgccttgctg cggcccaaga gtcggtaccg cccgcgacgg cgaccgtcac
      481 accgccgtca tcctcgacgc cgatgcccgg acccgggccg aaccactccc atgcgccctg
      541 gcctggaaga ggaccgggcc gcaggccacc gaactcgccg gagaacacgg acggctccgt
      601 cgcgaccgct gccccgcctg gcctgcgcca gtcggggccc gcggcttggg cgatggccgc
      661 gatcatctgc cgagccaaag cgcggatctc cggtgtggcc tgtccgggcg ccgggaagta
      721 gttgtcaact agcctagggt gcgctccgct gccaccaaac ggcgacgccg agtcgatcac
      781 cacattcccc tgcacgagca catcgtcgaa ctcggcgtcg aggtagatgg ccaggcagtg
      841 ggcccggtag acgaggttgt cgcggaacac gttcccgagc ccttggccaa agccgtagat
      901 ggcgcccccg tcggccggga gctgccacag cacgtcgtga accacgttgt gctcgaccac
      961 attgtcgcgg ccgtggagga agggcttgac gctctcgggc gtgaggggat tgtcgtcggg
     1021 gatctcatcc caccggaagt tgtacttggc tcggcccacg ccctcgcccc gctcgcggta
     1081 ctggttgaag tagctcgtgt gagcgccgct gatgcagatg ccggtgtagg gcatgtcgtg
     1141 aacccagttg caggcgatgt ggttgtggcc gctctggcgg acgtcgacgg cgccggcgtg
     1201 ccagaagtcg gtcgagctgt ggtggatgtg gttccgcgag atctggtggc cgtggttgac
     1261 atcgagcgtg ccggggccgt agccgccggc ctgaaggccc ccgcagcccg cgccgacgac
     1321 ctcgttgccg acgaagcgac agtcctgaca ccaacggtca aatcgggccg cgaagccgcc
     1381 gacatcctca aagaggcagc tctcaacggc gcaccgcgcg gcgccggcga ggtagacggc
     1441 gctgtcgagg gcgtgcaggt tggcctcatc ctcggccggg tgttcgtgcc agcgtgagcg
     1501 gtcaccgtgc cggaaggtca ggccgcggaa cgccacgtca tgcaccgtgt ccgcagggcg
     1561 atcactgccc acgaggcgga tgagctgctt gagagccggc gcgacaacct cagcggcctt
     1621 catgtcgacg ccggacaggg gcaggtagta gacggtgcct cgttccgagc tcagacacca
     1681 ttccccgggc tcgtcgatgg cctcgagcgt gttctcgacc cgaaatggat ccccgggcgc
     1741 gaagttgtag taggccatgg acttgagcac ggccagcgaa tgcgcctcat cgaccgactc
     1801 gagcgatgag atgaagttgg catagcggaa cgatggcagg ctgttgatct cgacatcgag
     1861 cgtgttctcg caccgcggga ttgtcccggg cgggaagtag aactggcggc tccccatgcc
     1921 ttcgggcgca tcgggctcgg gctctcctcc tcgggtcacc cggaaccact gcgcccagac
     1981 atcggtgtcg ggttcgcggg agcgcgtctg cttctcgccg ttcacgaaca gccgggtgaa
     2041 gtaccatcca cggggcacct tggccatcca tagacccccg cccgcgcggt gccagccggc
     2101 gatgcggcgg ccgccactga tgacgggatg ctcgttgggg aacgcctggt agacgacagg
     2161 ggcagcttcg gagcccgagt cctcgggcga gagggccagc ggctcgctga gcacgtatgt
     2221 gccaccgcgg accaggacgg tgaccgggcc gaggcgacga ttcgcgcgca gcgcgcgcag
     2281 ccggtcccgc gcaccggcga gcgtggccag cggtccgtcg gtccgcgccg ggttcggggc
     2341 cgccaggcga ccggtccagg tgtcccgccc gtcgggagag acgaagagac tcactggtgg
     2401 ctccgaggcg ggcgcaacgg cggtaagggc ggccagagcg aggaggtttc cgagggctcg
     2461 tgtcatgggg ggcctccgga gtgagtgagt cgagaaccgc ttccgaggaa ggcggtatct
     2521 cgcaatccgt ttgcctttcc gcgaagcggg ctgcttctcc tgcggcgacg cgcccggcgg
     2581 gttcgccatg ccgcctgatc ggccgaggag agttccgggg cccaccgccc tccgtcacgc
     2641 ggctgcacgc cgcgtttccc ggacgctggg actgggggat acgcgactgc gggcgacaga
     2701 gcccgtcgcc ggcggggctt gccgagtgcc cgaggaggcg cgggcgccgc gcccaggcct
     2761 tggtccggcg gcgaggccca ggggccaagg cggtgcaggg ccgtgtggcg aagcacgtga
     2821 ggacgatctg atggatgggg gccgggtgcg gatggcaggc atggttgact tcgcggccca
     2881 caatgaggat gtccgcgagg tctggcgtcg ctatcacgaa ggcaatccga gccgggtgcc
     2941 gatgatcatc ggcacgtcgg tgcgtttcac actgctcgac cggagcatca atgagaccgg
     3001 cgccgacttc gagcggttct tcaacgaccc cgacgtcatg ttcgagcggc atctgttcca
     3061 ccagtggtgg atccgtcatc atctaccaca agatgcggag atggggctgc ccgaggcgtg
     3121 gacggtcagc gtggactttc agaactcgta cgaagccatg tggttcggcg cgccgatccg
     3181 ctacttcgcc gatgacgtgc ccgactgccc accgatcgtg accgacgaga acaagtggac
     3241 cttcatcgac cacggcccac ccgaaccgtt ctccggttgg atggggcggg cgtgggagta
     3301 ctgggaatac ttccgcgaga aggccagaag ctacgagtac cgcgggcggc cggtccatgt
     3361 gggcggtgta ccgggttgtg gcacagacgg accattcacc accgcttgcg cgctgcgcgg
     3421 ggcgactgag atctgcctgg acatgtacgc cgaccccgac ttctaccacg cgctgatgac
     3481 gctcatcgtc gacgcgacca tcgggcggat gagggccttc cgcaaacgcc tcggggtgcc
     3541 tgtggattcg accgtctggg gcttcgccga tgacagcatt caactcctgt ccacgaggac
     3601 ctacgaggag cttgtgctgc cctaccatga acgcctgacg gcggagttcg gggcagaggg
     3661 gccaaactcg gttcatctgt gcggtgacgc gacacggcac ttccgtgtga tccgcgaccg
     3721 gctgaaagtg aactccttcg atacgggttt cccggtggac tttggtgctc tgcgggctga
     3781 cctgggcccc gatgtgcaga tctacggcgg gccgccagtt gccctgctgc tctccgggac
     3841 gccgcaggct atccgccagg aggtgcgccg catcctcggc accggcgtga ccgagggcgg
     3901 caggttcgtg ctgcgcgagg ccaacaacct ggccccgggg actcccatgg cgaacgtggc
     3961 tgcgatgtac gatgcatgca aggagttcgg gaggtacgag taggcgctga gtcggttgtc
     4021 atgggggcgg gggtgaacca tcggtccgac ggcccggagt gtcctgacgg ctcgcagcca
     4081 ccgcctactc acgccgactc cccccgctct cgatgaaccg ggggtattcg tcatgccgat
     4141 cgtggtattc gctccggcgc gagcggtcgg tatcgctggc ctgcctggct gcggggcgga
     4201 ttccccgaac cagcatttgg ggcaggaccg tcccgttcca ctcgttggcc tgggcggaga
     4261 agcacatctc gaccgtgtcg cccacgtgga actcttcggc gaggtcgccc atggagaagg
     4321 caatgccctc gaccagcatg gatgaggtgg gacccgatac acgcagcttg aggtgtgagc
     4381 catcgcccac gcgcttgagc agctcgatgc gggcctggcg gcagaggaac acgggttcgg
     4441 ggttgccggt cccaaagggt gcgaggcgtg ccagttcgtt gcagagccgc aggctgacat
     4501 cctcggggtt gagctcgcca tccactgtcc ggacggggcg caggagctcg tcgggcatcg
     4561 tttgccgggc gtaggcgttg atctcctggc ggaatcggtc gaggagctcg ag
//
LOCUS       DAOSPL010000136         4602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_18790_length_4602_cov_2.879701, whole genome shotgun sequence.
ACCESSION   DAOSPL010000136 DAOSPL010000000
VERSION     DAOSPL010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18790_length_4602_cov_2.879701"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PLQ54_03745"
     CDS             complement(<1..775)
                     /locus_tag="PLQ54_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOS92400.1"
                     /translation="MGLDPAGSPGTLTAPDALEAVTGIIFDIKRFAIHDGPGIRTTVF
                     FKGCPLRCRWCHNPEAVHGQPQLMFFPRNCIGCDRCIENCPRGAVVVRDGIRQILRDR
                     CQDCGTCTRECYARALVMSGREATAGEVVAEAERDAPFYENSGGGVTISGGEPFAQPV
                     FLDALLSLFRRRGRHVAVDTSGEAPFSVIEPMLDRIDLLLFDVKHADAEAHREWTGSS
                     NHRLLANLAKLGTRGVPLVARVPVIPGFNGSPEAIRAIAQ"
     gene            complement(777..2207)
                     /locus_tag="PLQ54_03750"
     CDS             complement(777..2207)
                     /locus_tag="PLQ54_03750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS92401.1"
                     /translation="MGRTRSGGQIPPEELDRLFREYAKTRAPEIRDRIVAQFSGLVES
                     VARSIPNTSEPTEDLVQEGYIGLIKAVDMYDVERGVKFITYATHLILGEIKHYLRDRK
                     SIIREPGWLYELNQKVSRAIDQLTQEHGRYPSVAEICKEVNIGEDAVLEVLRTRNTFQ
                     VGSLDVSDEDEESGRAHRLDTKKIRSLQHSTLELPVEDRIVLDSSLAKLKSIEREVIE
                     YFFFRDFSQTEIARRLGVSCNYVSHLVRAALRKLRSSLAREEQREASLRLRAEIERRR
                     AQLRLIEERGARDDLTGLRGLHYFEERLREEFLRAERYSHELAALLIDIDGLAQYNRR
                     ATAAAGDEALRRVAMLLGTNIRRVDVVARLEGGTFGLILPQTGDTAARLADRLVKRVA
                     QERIGATSPRGKYLTIKAGVAVYPVDALDDAGLIEASRRALNAAREAGGNGYVRAHAL
                     FSGLRATGTPSLLRSALAATRAAELE"
     gene            complement(2401..3633)
                     /locus_tag="PLQ54_03755"
     CDS             complement(2401..3633)
                     /locus_tag="PLQ54_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOS92402.1"
                     /translation="MPVFRYEAVDAAGNTVRSTITADSRDAVIQRMRELRYHPVDIAV
                     VTSEGGKMASINARFQGVKLKELVVFTRQFSTMIDAGMTILKSLDILAAQTKNSFFRE
                     TIQGVKLDIASGLSLADALAKHPRVFNDLYVNMIRAAEIGGILDVILQRIAGYLENEQ
                     EIRTKIKSAMMYPCVVFAFALLMTIGIVFFVLPQFKTIFAGLVGEDYKLPVLTTVLLN
                     SGATARQYWWFPILVIIGVVTAWKMVGRTESGRLKLDSFKLKIPIVGDLVLKISVARV
                     CRTLATLLASGVSILRCLEIVEATAGNAVIANAIRQARTAVHGGERICEPLAQSGIFP
                     LMTTQMVQVGEETGRLDEMLNKVAVFYEEEVDAMIKGLTSLIEPIMIVGLGGFVGFVA
                     VAVLKPIYDVLETMQQQK"
     gene            complement(3708..>4602)
                     /locus_tag="PLQ54_03760"
     CDS             complement(3708..>4602)
                     /locus_tag="PLQ54_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOS92403.1"
                     /translation="FEETRELDFSYGVKGLSRFRFNVYRQRGSVGAAVRAISDRIPTM
                     EEMNLPGIDVLKAFTELPRGLVLVTGPTGSGKSTTLATMINHVNQVRNVHVMTVENPI
                     EFIHKHNKSMINQRELGKDTLSYPAALRAVLREDPDVVLIGEMRDMETIEATLTVAET
                     GHLVFSTLHTNSAPATVDRIIDVFPPDQQEQVRVQLAGAIQGVVSQQLVPLAGGTGRT
                     CAIEFMVATSGIRNLIREGKSFHIQSAIETGKELGMITMDAALAELVLSGRVTREEAE
                     LKCLDKDNFNRLCSGSFGGRH"
BASE COUNT          795 a         1536 c         1430 g          841 t
ORIGIN      
        1 cctgggcgat ggcgcggatg gcctcgggcg agccgttgaa cccgggaatc actgggacac
       61 gagccacgag cgggacgcct cgagtcccca gcttcgccag gttggccaga agccgatggt
      121 tgctgctccc ggtccactcg cggtgggcct cggcgtcggc gtgtttcaca tcgaacagaa
      181 gcaggtcgat gcggtccagc atcggctcga tcacggagaa cggtgcctca cctgaagtgt
      241 cgacggccac gtggcggcca cggcggcgga acagggagag cagagcatcg aggaacacgg
      301 gctgggcaaa gggctcgcca cccgatatgg ttacgccgcc gcccgagttc tcatagaagg
      361 gcgcgtcgcg ctcggcttcc gcaacaacct cacccgcggt ggcctctcgg ccactcatca
      421 ccagggcccg cgcatagcac tctcgggtgc acgttccaca gtcctggcac cggtcgcgca
      481 ggatctgacg gattccgtca cggacgacga cggcgccccg agggcagttc tcgatgcacc
      541 gatcgcaccc gatgcagttg cgggggaaga acatcagctg cggctgccca tgtaccgcct
      601 cggggttgtg gcaccaccgg cagcgaaggg ggcacccctt gaaaaagacc gtggtgcgga
      661 tacccgggcc gtcgtggatc gcgaaccgct tgatgtcgaa gatgatcccc gttacggctt
      721 cgagggcgtc cggggcggta agggtgccgg gtgatccggc cggatccagt cccaactcat
      781 tccaactccg cagcacgagt tgcggcgagt gcgctccgca ggagcgatgg cgttcccgtg
      841 gcgcgcagtc cgctgaagag cgcgtgagca cggacgtagc cgttgccgcc tgcttctcgt
      901 gccgcgttca gggcgcggcg cgaggcctcg atcaggcccg cgtcatccag ggcgtccacc
      961 gggtacactg ccacacccgc tttgatcgtc agatacttgc cccgcggtga tgtcgcaccg
     1021 attcgctcct gggccacacg cttcaccaga cgatcggcca gccgggccgc cgtgtcgccg
     1081 gtctggggta agatgagtcc gaaggtgcca ccttccagcc gagctaccac gtcgacgcga
     1141 cggatgttcg tcccgagcag catggccacc cggcgcaggg cctcgtcgcc cgccgccgcc
     1201 gtcgcccggc ggttgtactg ggccagaccg tcgatatcga tcagcagagc ggccagctcg
     1261 tgtgagtatc gctcggccct caggaactcc tcccgaagtc gctcctcgaa gtagtgcagc
     1321 ccgcggaggc cggtcaggtc gtcgcgtgcg ccacgctcct cgatcagccg gagctgggcc
     1381 cgccggcgct cgatctccgc gcgaaggcgc agcgaggcct cgcgctgctc ctcccgtgcc
     1441 agcgagctcc gcagcttccg cagcgccgcg cggaccaggt gggacacgta gttgcacgat
     1501 actcccagtc tgcgggcgat ctccgtctga ctgaagtcgc gaaagaagaa gtactcgatg
     1561 acctcgcgct cgatggactt gagcttcgcc agcgagctat ccagaacgat gcggtcctcc
     1621 accggcagtt ccagcgtcga gtgctgcagg ctccggatct tcttggtatc cagccggtgg
     1681 gcacgccccg actcctcgtc ctcatcggag acatccagcg aacccacctg gaaggtgttg
     1741 cgggtccgca gcacttcgag caccgcgtcc tcgccgatgt tgacctcctt gcagatctcg
     1801 gccaccgacg ggtaccgccc gtgctcttgg gtgagctgat caatggcgcg gctgaccttc
     1861 tggttgagct cgtacagcca gccgggttca cggatgatgg acttccggtc gcgcagatag
     1921 tgcttgatct caccgaggat cagatgcgtg gcataggtga tgaacttgac cccacgctcg
     1981 acatcataca tgtccacggc cttgatgagg ccaatgtagc cctcctgtac cagatcctcg
     2041 gtgggctctg aggtgttggg gatgctccgg gccacactct cgaccaggcc cgagaactgc
     2101 gcgacgatgc ggtcgcggat ctccggtgcg cgggtcttgg cgtactcccg aaacagccgg
     2161 tccagttcct caggcggtat ctgcccgccc gatcgggtac gacccatcct ctagtctccc
     2221 tcgtgctctt cgtccctcag gcagaccggc acagcggccg gacacacgcg cgcacccagc
     2281 cccgggcacg gctcccacag cgtcccgccc ccggtctggc gtgctcgcac tgcctcttcc
     2341 tcgcacgacc cactttccga gtccggtgcc cgttctcctc cggtcccggc cgccccggtg
     2401 ctacttctgc tgctgcatcg tctcgagcac atcgtagatg ggcttgagca ccgccacagc
     2461 cacgaaaccg acaaagccgc cgagtcccac gatcatgatc ggctcaatca gcgacgtgag
     2521 ccccttgatc atggcgtcga cttcctcttc gtagaacacg gcgaccttat tgagcatctc
     2581 gtccagccga ccggtctcct cgcccacctg caccatctgg gtggtcatca gggggaagat
     2641 gccggactgc gcaaggggct cgcagatccg ctcgcccccg tggaccgcag tgcgggcttg
     2701 gcggattgcg ttggcaataa cggcattccc ggccgtggcc tccacgatct cgaggcagcg
     2761 gagaatcgac accccgctcg ccagcagtgt cgccaacgtg cggcagacac gggccaccga
     2821 gatcttgaga acaagatccc ccacgatcgg gatcttgagc ttgaacgagt ccagcttcag
     2881 cctccccgac tcggtgcgtc cgaccatctt ccaggccgtc acgacgccaa tgatgaccag
     2941 gatggggaac caccagtact ggcgcgcggt ggccccgctg ttgagcagga ctgtggtgag
     3001 tacaggtagt ttgtagtctt cgcccacaag gccggcaaag atcgtcttga actgggggag
     3061 aacgaagaac acgatgccaa tggtcatgag cagcgcgaag gcgaacacca cgcagggata
     3121 catcatggcc gacttgatct tggtgcggat ctcctgctcg ttctccaggt agcccgcgat
     3181 gcgctgcagg atcacgtcca ggatgccgcc gatctcagcg gcccggatca tgttcacata
     3241 caggtcgttg aacacccgag ggtgcttcgc cagtgcgtcg gccagactca agcccgacgc
     3301 gatgtccagt ttgaccccct gaatggtctc tcggaagaag gagttcttgg tctgcgccgc
     3361 caggatatcc aggctcttga ggatcgtcat gccggcgtcg atcatggttg agaactgccg
     3421 cgtgaacacg accagttctt tgagcttgac tccctgaaag cgggcgttga tgctcgccat
     3481 cttgccgcct tcggaggtga cgacggcgat gtcgactggg tggtagcgca gctcgcgcat
     3541 ccgctggatc acggcatcgc ggctgtctgc cgtgatggtt gaccttaccg tattgcccgc
     3601 cgcgtcgacg gcctcatatc ggaataccgg catcccgatc tccccttccc ggagtgaagg
     3661 gcactggcag gagcctcgac agccccgtac ggcagggccg gtcctcgcta gtgccggcca
     3721 ccgaacgaac ccgagcagag acgattaaag ttatccttat ccaggcactt cagttcggct
     3781 tcttcccgtg tcacgcggcc cgagagcact agctcggcca gggccgcatc catcgttatc
     3841 atgccaagct ccttgcccgt ttcgatggcc gactgaatgt gaaacgactt cccctcgcgg
     3901 atgaggttgc ggatgccgga ggtggcgacc atgaactcga tggcgcaggt tcgccccgtc
     3961 ccgcccgcca gaggcaccaa ctgctgactc accacaccct ggatcgcccc cgcgagctgg
     4021 acccgaacct gctcctgctg atcgggcggg aacacgtcga tgatgcggtc gaccgtcgcc
     4081 ggagcgctgt tcgtgtgcag ggttgagaac acgagatggc cggtttcggc cacggtcagg
     4141 gtggcttcga tggtctccat gtcgcgcatc tcgccgatga ggaccacatc cgggtcctct
     4201 cgcaacacgg cgcggagtgc cgcgggatag ctcagcgtgt ccttgcccag ttccctctgg
     4261 ttgatcatgc ttttgttgtg cttgtgaata aactcgatcg ggttctcgac cgtcatcaca
     4321 tgcacgttcc ggacctggtt cacgtggttg atcatggtgg ccagcgtggt cgacttgcca
     4381 ctgcccgtgg gtcccgtgac caacaccaga ccacgcggga gctccgtgaa ggccttcagg
     4441 acgtcgatgc cgggaaggtt catctcctcc atggtcggga tgcgatccga aatggcccgg
     4501 acggcggcgc cgaccgagcc ccgttggcgg tacacgttga agcggaagcg gctcaggccc
     4561 ttgacgccgt agctgaaatc cagctcgcgg gtctcctcga ac
//
LOCUS       DAOSPL010000137         4572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_18966_length_4572_cov_2.742307, whole genome shotgun sequence.
ACCESSION   DAOSPL010000137 DAOSPL010000000
VERSION     DAOSPL010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18966_length_4572_cov_2.742307"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..753)
                     /locus_tag="PLQ54_03765"
     CDS             complement(151..753)
                     /locus_tag="PLQ54_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92404.1"
                     /translation="MALPGVVIHITDELQPEISIQRAVEGLGLSYLAFYGIRDALRAV
                     AHTHDEGRLVALVTDLSLRPPGHLHGRDEMVGVDLVRWLKGSDRVRTRFYDCLLYDLI
                     QALASSTAASAERENARHAWLDFSRLLLSCEQRRSSGDGTAPVLDAPIVVFSEHANDP
                     RVVEDLSGIGGVVLVPKSEGPEAVARVLRALPGTATAPRE"
     gene            complement(939..1196)
                     /locus_tag="PLQ54_03770"
     CDS             complement(939..1196)
                     /locus_tag="PLQ54_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92405.1"
                     /translation="MNVRAGDPLVRIRLYGLEHDVPVGDLRLTRCASDSAVLSAVARY
                     LDVSYAGLAQARVVREMAGHLTVKPKSPITRGIERRDRAFY"
     gene            1707..4196
                     /locus_tag="PLQ54_03775"
     CDS             1707..4196
                     /locus_tag="PLQ54_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOS92406.1"
                     /translation="MARAAHSAVVPLAVCLALGPWLRPASGAEHLGTASVLAYTSEID
                     GTRQEYGAFVPAVGPPRPEGYPACLHYHGYGWNVSAGFSDFQERWAYDHGWILANVNG
                     RGPTFYDGIGENDVFRVLQDLDGRFGIDKRRVYATGGSMGGTGAYRQGVRWPDSFAAV
                     VGVDGWSDFRLFHWHWYGPTYAPSGIEEFRRPLLEAISSSYIAPRARTGNIGLVTDLL
                     DTVVWPGEGQLLDESLDIFERESPHEYLHSATENPTIGHGGGFRIEEIYRYFLDKTSL
                     ERPAGIRVVTTQLKYGGLHWAAIEEFIQPGVRADLVVDASGGTVFVQATNVAGFAVNL
                     PNSPVAQHDSVRIVVNGELAYTGRPEVVTLALADPRDAASGWRRASDAPALRKTAALE
                     GPIGHAFTRPFVLAYGTAGTPEETRQNREEAEQFAESWTRYYVRYPAVRAVRDVDVLA
                     DQVRGASLILFGTEESNRLLREAAARYVLPVRVSREGIDVRDARYGDRCYRGAGFGTF
                     FVYPNPLSAFRTYMVVAHGRYPTLPDGQRPQGLGYDLEKLPWTWPDYVVFNGDPARLP
                     YVGNVNDKAEVLCYETAYFVEAGYFDQRWQLMRDLELGWVNQDPDAGAARRLHVGELA
                     VDARREGIGWVGEAALRAVDESGAPMEAVRVAVEWSGSARVSDAGVTGADGWVRFQCG
                     PVTIPRGTFTCRVLSMMGTDATYDWSRNARHQASRGFGHARDVRVWFHSPLDAVRLGS
                     VQRVDIGVANHGDAAAECTVRLIAGQGEVDPHAQTVRLAAGESRAVTFWWSTGGLEPG
                     QVRLRAQAELHGGTDSVPDDNEQERAVRLSL"
     gene            4227..>4572
                     /locus_tag="PLQ54_03780"
     CDS             4227..>4572
                     /locus_tag="PLQ54_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92407.1"
                     /translation="MRRLALVAALVGLGLAVSDVAAQVSVGVSEEPAETEVVVYAGRN
                     ATLVRQQRELPLVAGDNTVEFRWRDVTIDPASIALDPGPDAEVVSSVFPAGLDRVLRF
                     TVRSSRAGVMPAR"
BASE COUNT          713 a         1494 c         1619 g          746 t
ORIGIN      
        1 tcttcgacgt gcccaccaag acgcaggtga acctgacggt gaacagcacg tcggtcggga
       61 cagctgcgac gaccacgcaa gtggcggatc tgaccttcgc cggccccgca acaggcacgg
      121 tacgccccat cagcagcgcg acagtgagct tcactcacgt ggagccgtag cggtaccggg
      181 tagcgctctg aggacgcgcg ccacggcttc gggcccctcg gacttcggta ccaggaccac
      241 cccgcctatc ccggacaggt cctcgacgac ccgggggtcg ttggcgtgct cggagaacac
      301 gacgatgggc gcatcgagca cgggcgcggt gccgtccccg ctggacctgc gctgctcgca
      361 cgagagcagc aaccggctga aatcgagcca ggcgtggcgc gcgttctccc gctcggcgga
      421 cgcagctgtt gaggaggcca gggcctggat caggtcatac aggaggcagt cgtagaagcg
      481 ggttcggacc cggtcggagc ccttgagcca ccggaccaga tcgacaccca ccatctcatc
      541 gcggccgtgg aggtgacccg gagggcggag ggacaggtcg gtgacgagag cgaccagcct
      601 gccctcatcg tgggtatggg cgacggctct cagcgcgtcg cgaatgccgt agaaggcgag
      661 gtacgaaagg ccgaggccct cgacggcgcg ctggatgctg atctcgggtt gtagctcatc
      721 ggtgatgtga atgaccacgc cgggcaacgc catgggactt cacccttccg cttccgtgcc
      781 ccgtgctccc tcagctagcg ggattcggag cgcgaggcgg cgggccctgc cgatccacca
      841 gccgccggca tgggcggtac gcgaagcggc ccgggcgccc ggtcatgtca cggccaggtc
      901 ccgggccgca cgacggcgga gcccagcgag ccgatcgctc agtagaaggc gcgatcccga
      961 cgctcgatgc cccgggtgat ggggctcttg ggcttgacag tcaggtggcc cgccatttca
     1021 cgaaccaccc gagcctgtgc cagcccggcg tagctcacgt cgaggtagcg tgccacggcg
     1081 ctgagcacgg cggagtccga agcacaccgg gtcaagcgga ggtcaccgac gggcacatcg
     1141 tgctccagcc catacaagcg aatgcgtacc aacggatccc ctgcacgaac gttcatctcg
     1201 gttccctcct agaactgccc gggtctaggc ggcgcccggg ggctaccgcc tcaccccccc
     1261 ccaccacatc ccctccgcgg atggacagtt ggacgcttcg gcgcggggcg tggttcgagc
     1321 cagagccaac ttatctttgc ctgcaccacg gccggaacac ggggccgggt cgcgtgtcca
     1381 aaagcgcccg aacggcgctc ggcgacggtg cgcgtcgtgc gcccccgtac ctgtggcact
     1441 cctcttctgt cgtgtcccgg cggctcggac taaaggggtc atcggcccgg agagcgaacg
     1501 ggaggcggcg gccaggcgca aaaaaacgcg aggagctttt gagggctcct cgcagcctag
     1561 aggagtaagc cagtgaagcg gggagtcagg ccgcttcatc tagtacaacg tttgggcgcg
     1621 ccaaatgttt caagtctggc gcagttcttt cgtgggcggt gccctagatg tcgcccaatc
     1681 gccgtccgtc tggcgtgatc ccgtccatgg cgcgagcagc ccactctgcg gtcgtacccc
     1741 tggcggtgtg cctggccctg ggcccatggc ttcgcccggc ctccggtgct gagcatctgg
     1801 ggaccgcgtc ggtactcgcc tacacgtccg aaatcgacgg gacccggcag gagtacggcg
     1861 ccttcgttcc ggcggtaggc ccgccccggc ccgagggata tccggcgtgc cttcactacc
     1921 acggctatgg gtggaatgtg tcggccggct tttcggactt ccaagagcgg tgggcgtacg
     1981 accacggatg gatcctggcc aacgtgaatg gccgcgggcc gaccttctac gatggcattg
     2041 gcgagaacga tgtcttccgt gtgctgcagg acctcgatgg tcgcttcggc atcgacaagc
     2101 gccgggtcta cgccaccggc ggcagcatgg gtgggacggg tgcgtaccgc cagggggtgc
     2161 gctggccgga cagcttcgcg gccgtggtgg gtgtcgacgg gtggtctgac ttccggctgt
     2221 tccactggca ctggtacggc ccgacgtatg ccccctcggg catcgaagag ttccggcgcc
     2281 cgctgctcga ggcgatcagc tcgagctaca tcgccccccg cgcgcggacg gggaacatcg
     2341 gcctcgtgac cgatctgctc gatacggtgg tctggcccgg cgaggggcag cttctggatg
     2401 agtcgctgga catcttcgag cgcgagtcgc cccatgagta tctccactcg gcgacggaga
     2461 accccactat cggccacgga ggcgggttcc ggatcgagga gatctaccgc tatttcctcg
     2521 acaagacgtc gctcgagcgc ccggcgggga ttcgagtcgt gacgacccag ctgaagtacg
     2581 gcgggctgca ctgggccgcc atcgaggagt tcatccagcc aggcgtccgc gccgacttgg
     2641 tggtcgatgc ctctggcggc accgtgttcg tgcaggcgac gaacgtggcc ggattcgccg
     2701 tcaacctgcc caacagcccg gtcgcgcagc acgattcggt ccgcatcgtt gtcaacggcg
     2761 agctcgcgta caccggccga cccgaggttg tgacgctcgc tctggccgat ccgcgcgacg
     2821 cggcctcggg ctggcgccgg gcgtcagacg ctccggcgtt gcggaagacc gcggcactcg
     2881 aaggaccgat cgggcacgcg ttcacgaggc ccttcgtgct ggcttacggg actgcgggca
     2941 ctcccgaaga gacccggcag aaccgcgagg aggcggagca gttcgccgag agctggacac
     3001 ggtactacgt ccgttacccg gccgttcgtg ccgttcggga cgtcgacgtg ctcgctgacc
     3061 aggtccgggg agccagcctg attctgttcg gcaccgagga gagcaaccgg ctgctccgcg
     3121 aagccgcagc gcgctatgtg ctgcccgtgc gggtgtcaag ggagggcatc gatgttcgtg
     3181 atgcccggta cggcgatcga tgttaccgcg gcgccgggtt cgggaccttc ttcgtatatc
     3241 ccaatccgtt gtctgccttc cgcacgtaca tggtggtggc gcacgggcgg tacccgacac
     3301 tgcccgacgg ccagcggccc cagggtctcg ggtatgacct tgagaagctc ccctggacgt
     3361 ggcctgacta cgtggtcttc aacggcgatc cggctaggct gccctatgtg ggcaacgtca
     3421 atgacaaggc cgaggtactg tgctatgaga ccgcctactt cgtcgaggcc ggctacttcg
     3481 accagcgctg gcaactcatg cgcgacctgg agctcggctg ggtgaaccag gaccccgacg
     3541 cgggcgccgc gcggcggctt cacgtgggcg agcttgccgt tgatgcccgc cgcgagggta
     3601 tcgggtgggt cggggaggcg gccctgcggg ccgtggacga gtccggggcg cccatggaag
     3661 ccgtgcgcgt tgccgtcgag tggtcgggca gcgcccgcgt ctccgatgcc ggtgtgacgg
     3721 gtgcggacgg ctgggtgcgc ttccagtgcg gacccgtgac catacctcgt ggcacgttta
     3781 cctgccgcgt tctgagcatg atgggcaccg acgccaccta tgactggtcc aggaacgcgc
     3841 gacaccaggc gtcgcgcggg tttggacacg cgcgcgatgt ccgcgtgtgg ttccactcgc
     3901 cactcgacgc cgtgaggctg gggtccgtcc agcgggtcga catcggcgtc gcgaaccacg
     3961 gtgacgcggc cgcggagtgc acggtgaggc tcatcgcggg acagggcgaa gtcgatcccc
     4021 acgcacagac cgttcggctg gccgcgggag agagccgagc cgtcacgttc tggtggagca
     4081 cgggtggcct cgaaccgggg caggtccggt tacgggccca ggccgaactc cacgggggaa
     4141 ctgactcagt tcccgatgac aacgagcagg agcgggcggt gcggctctcc ctgtgacggg
     4201 cgtgccgtca ggcaggaggt ctggtcgtga ggcgactcgc cctggtggca gccttggtgg
     4261 gtctcggtct ggcagtctca gacgttgccg cgcaggtcag tgtgggcgtg tccgaggagc
     4321 cggcggagac cgaggtcgtt gtgtacgccg gccgtaacgc gacactggtg cgacagcagc
     4381 gcgagcttcc gctggtcgcg ggcgacaaca cggtcgagtt ccggtggcgg gatgtcacca
     4441 tcgacccggc ctcgattgcc ctcgatcccg gcccggatgc cgaagtggtg tccagcgtgt
     4501 tcccggcggg cctggatcgg gtgctgcggt tcacggtgcg gtcaagccgg gccggcgtca
     4561 tgcccgcccg ga
//
LOCUS       DAOSPL010000138         4551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_19093_length_4551_cov_3.228648, whole genome shotgun sequence.
ACCESSION   DAOSPL010000138 DAOSPL010000000
VERSION     DAOSPL010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19093_length_4551_cov_3.228648"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /gene="surE"
                     /locus_tag="PLQ54_03785"
     CDS             complement(<1..339)
                     /gene="surE"
                     /locus_tag="PLQ54_03785"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOS92408.1"
                     /translation="MILLTNDDGVRAEGLVRLAEALDGLGAIHVVAPDRQRSAIGHAI
                     TLDTPLKVEEVALSAGLRALACSGTPTDCVMVAVHGLLPALPDLVVSGINHGANMGED
                     LTYSGTVSAAM"
     gene            complement(413..1681)
                     /locus_tag="PLQ54_03790"
     CDS             complement(413..1681)
                     /locus_tag="PLQ54_03790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS92409.1"
                     /translation="MRQPAPSTDPIVRHPSPRRGRHNYPLLFADAVGWPLGWMFMSID
                     VILPTFLGHLTPSLRAVSYIRGIYSLGVWLPSLWAPNLIRGLRRRGPYVVVIGLIERV
                     PLLAVAVATWLWGRTNPSLVLGVFFVAWAIRSAAEGVNRPAYSQLVAEGLPPRVRGRF
                     WGYAGAICGALAVPYTLWAAGWLKRTPFPNGYVVLFLIGFTVLAVTLLPIWFVRERMS
                     GRPRSGDSGTSLRSMRLLLDDRNFGLMTLAVVVLACADMAAPFYVRQAMDHLGAPEHY
                     AALYAGVQSGAGSIAALLAGLWVSRRGNRPALFMAGLVGALAALAALAIASPIAYTAV
                     FALLGATSAVVGMYAYNLLLALPAPSRVAVYSGVHYTVVEPARSIMPVIGGEVAQRLG
                     PSAVFLPAACAYLLWCVILLAVREPEHHLG"
     gene            complement(1754..3304)
                     /locus_tag="PLQ54_03795"
     CDS             complement(1754..3304)
                     /locus_tag="PLQ54_03795"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011012073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOS92410.1"
                     /translation="MSRRVYVFDTTLRDGEQSPGASLNPREKLEIARQLDRLGVDIIE
                     AGFPVSSPAEIDSIQAICREVRRPTICGLARVVEKDIDAAAKALEGAQRSRIHVFIGT
                     SPVHRESKLKKSEAEVVAMAVHGVEYAKARCHEVEFSAEDATRTPREFLKEVFTAAIA
                     AGATTINVPDTVGYTCPTEYYDLIAWLIANVPGMANVHVSVHCHDDLGMAVANSLAAV
                     SAGATQVECTVNGIGERAGNASLEEIVMAMRTRSDLFPTETGVAPGEIMRASRMVSQF
                     TGIVVQPNKAVVGSNAFAHESGIHQDGVLKERTTYEIMDPEAVGAGGTKIVLGPRSGR
                     HALRHRLGELGYQVSDADMGPIYERFLAIADAKKQVFDEDLVAIMEEHDVSSAPGFEV
                     VGLQTISGSRAIPTATVEVRTPGGDTVRESAAGSGPVEAVYAAIDRATGLSCRLLEYR
                     LRAVRENRDAVGEARVKVEHQGRTAVGLGASTDVVEASARAYVSAVNRIRQVEANGKD
                     RPAAEGVS"
     gene            3964..>4551
                     /locus_tag="PLQ54_03800"
     CDS             3964..>4551
                     /locus_tag="PLQ54_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92411.1"
                     /translation="MGAASIEQQCFVIVLDPSGTDFATFRSCVENQFDLGDYSRAIPT
                     PGQPVAIYQGPEGRQLRIEGQRIVIEGAAWEREAGRLARCLEELCAVTGVRVTASGVN
                     PIFSVELSPAEAASELCEPGPRIRRLMGDQAAFAAVTVTAPRGSGRLSFEFCPGPGPG
                     PVLRVLGNWHVDHGSVEECVRRLQAYPELVRELQRD"
BASE COUNT          760 a         1547 c         1549 g          695 t
ORIGIN      
        1 catggcggca gacacggtcc ccgagtaggt gaggtcctca cccatattgg cgccgtggtt
       61 gatgccggac actaccagat cgggtagggc gggcagtagg ccgtgcacgg caaccatcac
      121 gcaatccgtg ggtgttccac tacaggcgag ggctcgtaga cctgcggaga gcgcgacctc
      181 ctctaccttc agcggcgtgt ccagcgtgat cgcatgccca atggcgctgc gctggcggtc
      241 cggggccaca acgtggatgg cgccgaggcc gtcgagagcc tcagcgagcc gcaccagccc
      301 ctcggcgcgc acgccatcgt cgttggtcag caggatcagt cgctcgtcgg tggtcaccag
      361 atcagagcct ttccgccaca gagtctacac ccgttgcggc gcggcgggtg actcatccca
      421 agtggtgctc gggctccctc acggccagca ggatcacaca ccaaagcagg tacgcgcagg
      481 ccgcggggag gaagacggca cttgggccca gtcgctgggc gacctcgccg ccaatgacgg
      541 gcatgatcga ccgggccggc tcgaccaccg tgtagtgaac gccggagtac acggccaccc
      601 gggacggcgc aggcagcgcc agcaacaggt tgtacgcgta catgccgact acggcggacg
      661 tggcgcccag gagcgcgaac accgcggtgt aggcgatggg gctggcgatg gccagcgccg
      721 ccaaggcggc cagagcccct acgagcccgg ccatgaacag cgccgggcga ttcccacggc
      781 ggctcaccca cagccccgcc aggagcgcgg cgatcgagcc ggcacccgac tgcacgcccg
      841 cgtacagcgc cgcgtaatgc tccggcgccc ccaggtggtc catggcctgc cgcacgtaga
      901 agggcgccgc catgtcggca cacgccagga ccaccaccgc cagagtcatg aggccgaagt
      961 tccgatcgtc gaggagcagc ctcatggatc gcaagctcgt gcccgagtcg ccggaccggg
     1021 gacgtcccga catccgttcc ctgacgaacc agatcggcag cagggtgacg gccaggacgg
     1081 tgaagccaat gaggaacagg acgacatatc cgttcgggaa cggcgttcgt ttgagccagc
     1141 ccgcggccca gagcgtgtag ggcacagcca acgcgccgca tatggctccc gcgtaacccc
     1201 agaagcggcc gcggacccgc ggcggaaggc cctcggccac gagctgcgag taggcgggcc
     1261 gattcacccc ttcggccgcg gagcggatgg cccatgcgac gaagaacacg ccgagcacga
     1321 gcgaggggtt tgttcgtccc cacagccagg tggcgaccgc taccgcgagc agcggcacgc
     1381 gctcgatcag tccaatgacc accacgtagg gtccgcgccg gcgcagtccg cggatcaggt
     1441 tcggcgccca caagctgggc agccacaccc ccaggctgta gatgccgcgg atgtaagaca
     1501 cggcccggag cgagggggtg agatggccga ggaaagtcgg aaggatgacg tcgatggaca
     1561 tgaacatcca accgagcggc cagccaacgg cgtcggcgaa cagcagcggg tagttgtgac
     1621 gcccgcggcg tggtgagggg tggcgcacga tcgggtccgt actgggggca ggttggcgca
     1681 agggtccctc gcatcaggca caacagaagc ggccggctgc ctggtgcggc cggccgcggg
     1741 agtagctctg actctaactt accccttcgg cggccgggcg atccttgcca ttggcctcga
     1801 cctgacggat gcggttcacg gccgacacat aggcccgggc gctggcctcg acgacgtcgg
     1861 tcgaggctcc cagacccacg gcagtccggc cctggtgctc caccttcacc ctcgcctcgc
     1921 ccaccgcgtc gcggttctcg cgcaccgcgc gaaggcggta ctcgagcagc cggcacgata
     1981 ggcccgtggc ccgatcgatg gcagcataca cggcctccac cggcccggag ccggcggcgg
     2041 actcacgcac cgtgtctcca cccggcgtcc ggacctccac cgtggccgtg gggatggccc
     2101 tgctgcccga gatggtctgc aagccgacga cttcgaaacc tggggccgac gagacatcgt
     2161 gctcctccat gatggccacc aggtcttcgt cgaacacctg cttcttggcg tcggcaatag
     2221 cgaggaagcg ctcgtagatg ggacccatat cggcatcgga gacctggtag cccagctctc
     2281 ccagccgatg gcggagcgca tgccggccgg atcgcggccc gaggacgatc ttggttccac
     2341 cggccccaac ggcctcgggg tccatgatct catacgtggt ccgctctttg agcacgccgt
     2401 cctggtgaat ccccgactcg tgagcgaagg cgttcgaccc caccacggcc ttgtttggtt
     2461 gcaccacgat gccggtgaac tggctcacca tccggctcgc gcgcatgatc tcgccgggcg
     2521 cgacgcctgt ctcagtcggg aacaggtctg accgcgtacg catggccatg acgatctctt
     2581 cgagggaggc gttgccggcg cgctcgccga tgccgttcac ggtgcactcg acttgcgtgg
     2641 ccccggcgct caccgcggcc agcgagttgg ccacggccat acccaagtcg tcgtggcagt
     2701 gaacactcac atgcacgttg gccatgccgg ggacgttggc gatgagccac gcgatcaggt
     2761 catagtactc ggtggggcag gtgtacccga cggtatcggg cacgttgatg gtggtcgcgc
     2821 ccgcggcgat ggctgcagtg aacacctctt tgaggaactc gcgcggagtc cgtgtggcgt
     2881 cctctgccga gaactcgacc tcgtggcacc gggccttcgc gtattcgacc ccatgcaccg
     2941 ccatggccac gacctcagcc tcggacttct tgagtttgct ctcgcgatgc acgggcgagg
     3001 taccgatgaa cacgtgaatg cggctccgct gggcaccctc cagcgccttc gccgcggcgt
     3061 cgatgtcctt ctcgacgacc cgagccaggc cgcagatggt tggccggcgg acctcgcggc
     3121 agatggcctg gatggagtcg atctcggccg gagacgagac cgggaagcct gcctcaatga
     3181 tgtcgacacc caggcggtcg agctggcggg cgatctcgag tttctcacgt gggttcaggc
     3241 tggctccggg cgattgctcg ccatcgcgca gggtggtgtc aaacacgtag acgcgcctgc
     3301 tcatggctgt gtcctctcgc atcgggattg ggtcagagcc cctcggacat gcggcagcgg
     3361 atgacttcgt ccgcacccgg ctggccgctg gacaccgggt acggcggtcg taggatgagt
     3421 gccatgcggc ccacctcccg aatcgaaaag ccccccgtct ccgttgagac gaggggccac
     3481 atccccacgc ggtaccactc aacttggcgc ccgccccgct ggcccagcca gcgaaacgga
     3541 cgtcctctct cgacacactg cctcacggca ggtgccgcgc ggctcgatgc cgggcccccg
     3601 gtaacggtgg ggcgttccgt cggtccctac tccggcgcac cagtcggcgg ccgtttcggt
     3661 ccgcggctcg agggcgagat tcggaagcgc cgacgggcac cgcctctcag ccaagggcgg
     3721 ctctctgtag ccccgccact tcctactgct ccctgtccat gcctttgaga ctattcggct
     3781 gtgattccga tgatatcctc gcacggcgcg cgtgtcaatg ctgggcgatc gaaggaggat
     3841 gaccggctcg tcgctggtga agcgcttcgg cgtccagagg gtggcgcagg gcctcgggtc
     3901 acctgtctcg aacagatgga gcgcggacag cgcagcggcg ccggatccgc agcggagggc
     3961 acagtgggcg cggcttcgat cgagcagcag tgtttcgtga ttgtcctcga cccctcgggg
     4021 acagacttcg cgaccttccg gtcgtgcgtg gagaaccagt tcgacttggg cgactactcc
     4081 cgggccatac cgacgcccgg tcagccggtg gccatctacc aggggccaga gggccgccag
     4141 ctccgcatcg aaggccagcg catcgtcatc gagggagcgg cgtgggagcg cgaggccggc
     4201 cggctggcgc ggtgcctcga ggagctgtgc gccgtcacag gcgtgcgggt cacggcttcg
     4261 ggtgtgaacc ccatcttctc agtggaactc tcaccggccg aggccgcaag tgagctctgc
     4321 gaaccgggac cgcggatccg gagactgatg ggcgaccaag cggccttcgc cgcggtgacg
     4381 gtcaccgcgc cgcgcgggag cggccgactg agcttcgagt tctgccccgg accgggaccc
     4441 gggccggtgc tgcgagtgct cggaaactgg catgtggacc atggttcggt cgaggagtgc
     4501 gtccggcgcc tccaggccta ccccgagctc gttcgcgagc tacagcggga c
//
LOCUS       DAOSPL010000139         4538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_19172_length_4538_cov_3.390141, whole genome shotgun sequence.
ACCESSION   DAOSPL010000139 DAOSPL010000000
VERSION     DAOSPL010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19172_length_4538_cov_3.390141"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="PLQ54_03805"
     CDS             <1..1038
                     /locus_tag="PLQ54_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019542590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS92412.1"
                     /translation="SIFRPLGEYVGIAGFVSVVMTGMIEISAGRLLLSELVPLALLIQ
                     RVATALNKAGRGAAMLQQASAICERAFELLDTPPEEPDPPDAVELPQGPGHVAFEEVR
                     FAYSPGEPILDGISFEVPPGRRVAVVGPSGAGKSTIANLLPRLYEVTEGRIVVNGHDI
                     RRVTRASLRSQIGVVLQDTYLFSGTIRENILVGRPEASEAEMLEAARSAHADEFIRVL
                     PQGYDTPVGERGVTLSGGQRQRVAIARALLRDPRILILDEATSSLDSESEAVVQAALE
                     VLMADRTTLVIAHRLSTISGADEILVLDQGRIVERGTHESLLAGDGQYAALWRIQSGD
                     RRVPSPGSMQP"
     gene            1035..2402
                     /locus_tag="PLQ54_03810"
     CDS             1035..2402
                     /locus_tag="PLQ54_03810"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HOS92413.1"
                     /translation="MTADSSSGSAEHPQRVGGAQWWYRLGLAALAPALCGFFAHRITL
                     GGKPTVGLRERLGWLPESVVAACRRSPGDRMVWIHAVSVGEAVPAQALAAAIQSIDSS
                     VRIVVSSTTPSGREMAQSRLPFACAFFYLPYDLPWCVERTLDAVSPDALVIMETELWP
                     NLLAAARERGIRTMIANARISDRAFGRFLRFRWLYRWVLSNVDLVAAQSPLCAQRFLD
                     LGARPGRVQVLGNLKFDEARPDVSEDQCRELRGRLGFDERDLVLVFGSTAPEENEALL
                     DAFAVIRAEEPRARLIMAPRHLERVPDCVRAAEQHGFRTILRSQLPADGDNDRIVVLD
                     TFGELATLYALSAVAFIGRSLVPLGGGNLLQPLGFGVPVVFGPHMENFREAARSALEA
                     GLAFEVASSGDLAEVCCRLLRSPDERARIAERAPAFLADNAGAAGRHAEALAGLLGWC
                     GTEAQ"
     gene            2399..3478
                     /gene="lpxK"
                     /locus_tag="PLQ54_03815"
     CDS             2399..3478
                     /gene="lpxK"
                     /locus_tag="PLQ54_03815"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOS92414.1"
                     /translation="MNLAMQWVELVEQGPRNAQGRLAHLCLRGMSVFYGLALDANLEL
                     YRRAILKRHPAGLPVISIGNLTLGGTGKTTFTQMLARQLLDLGQRPAILLRAYKRGLR
                     DHCDEYEVYRAGLPEAVQILEGRNRVASAARARSEGATVALLDDGHQYLKLSKDLRIL
                     LFDCTQPRHLDRLFPAGMLRQPLSHVATADLVGLTRCDQAGPEAVARMRARLRSAGAQ
                     GDFVECAHRPTCLVDGRTGERLGARELAGQRVVAVSGIGNNEAFGRTVASLGVEVARH
                     LRFPDHHAFSARDADHITAEVESRQVSCVVTTAKDAPRLAPVLRRVDFVAVRVEMEIL
                     SGAELLRARLSSIGLTPGEPGAAHA"
     gene            3471..>4538
                     /locus_tag="PLQ54_03820"
     CDS             3471..>4538
                     /locus_tag="PLQ54_03820"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOS92415.1"
                     /translation="MPDRDLAALHAYADWRTRRTRALLPWLRLKALTRGPQGRQRLSA
                     GLGHVTPGCMCPRSCRHLVLYCHNIGEARTALPLVVGLRKRDPMLRYVVWAKSVAAFE
                     AAPGILPEPCAVLYAPLDGTQAVRRALERCRALALVILEGDLRPTMDWGAREAGIPTA
                     FVSAGLSPVEMARHRGHREGQKQMLEALDLLALKSEADAQRAIELGVDRGHAWVSGNC
                     RFDVGRLDLTPPRGPLVEFIEGARLIGRVVMVAGSTYGREERYLAEVRSAPRLAGSLL
                     LVVAPRKPGRADEVLATFRRAGARVARRTELGTGSGPLDLPADVLVLDTMGELLTTYG
                     LADVCFVGGSLVNKGGHNLIEA"
BASE COUNT          652 a         1529 c         1594 g          763 t
ORIGIN      
        1 agcatcttcc ggccgctcgg cgagtacgtc ggtatcgccg ggtttgtgtc ggtggtcatg
       61 accggcatga tcgagatctc cgcggggcgc ctgcttctga gcgaactggt cccgctagct
      121 ctgctgatac agcgcgtcgc caccgcactc aacaaggccg ggcggggcgc ggccatgctc
      181 cagcaagcga gcgccatatg cgagcgggcg ttcgaactgc tggacacgcc ccccgaggag
      241 ccggatccgc cggatgcggt cgagctgccc cagggccccg ggcacgtggc gtttgaggag
      301 gtgcgcttcg cctactcgcc cggcgagccc atactcgacg gcatcagttt cgaggtgcca
      361 ccaggtcggc gggtggctgt cgtcggcccc agcggcgccg gcaagtccac gatcgccaac
      421 ctcttgccgc gcctctacga ggtcaccgag gggcgcatcg tcgtgaacgg ccacgacatc
      481 cggcgagtga cgcgtgcatc cctgcggtct cagattggcg tcgtcctgca ggacacgtac
      541 ctgttctccg gcacgatccg tgagaacatc ctcgtcgggc ggcccgaggc gtcggaggca
      601 gagatgctcg aggccgcgag gtccgcccat gccgacgagt tcattcgcgt gctgccccag
      661 gggtacgaca cgccggtggg agagcggggg gtcacgctca gcggcggtca gcgccagcgt
      721 gtggccattg cccgggccct tctacgcgac ccacggatcc tcatcctcga cgaggcaacc
      781 tcttccctcg attccgagtc ggaggcggtc gtccaggcgg cactcgaggt actaatggcg
      841 gaccgaacga ctctggtcat cgcccaccgg ctgtcgacca tctcgggagc cgacgagatc
      901 ctggtgctgg accaggggcg gatcgtggag cggggcacac acgagagcct tctggcgggc
      961 gacgggcagt atgcggcgct gtggcgcatc cagtctggag accgccgggt cccgtcgccc
     1021 ggctcgatgc aaccatgacg gcagacagct ccagtggcag cgcggagcat ccgcagcgcg
     1081 tgggtggcgc gcagtggtgg taccggctcg ggctggctgc gctcgcgccc gccctctgtg
     1141 gcttcttcgc ccaccggatc acgctgggag gcaagccgac cgtcggcctc agagaacgtc
     1201 tcggctggct tccagagagc gtggttgcgg cctgccgccg gtcgccgggt gaccggatgg
     1261 tgtggattca tgccgtgtcg gtcggagagg ccgttcccgc ccaggctttg gccgccgcca
     1321 tccagtcgat tgactcctcg gtgaggatcg tggtctcgag cacgacccct agcgggcgtg
     1381 agatggccca gtcgcgcttg ccattcgcct gtgccttctt ctacctgccc tacgacctgc
     1441 cctggtgcgt cgagcgcacc ctggacgccg tctctccgga tgctctcgtg atcatggaga
     1501 ctgagctgtg gcccaaccta ctggccgcgg ctcgagagcg ggggatccgc accatgatcg
     1561 ccaacgcacg gatctccgac cgagccttcg gccgattcct gcgcttcagg tggctctacc
     1621 gctgggtgct ctccaacgtg gatcttgtgg ccgcccagtc ccccctgtgc gcccaacgtt
     1681 tcctcgatct gggcgcacgg cccgggcgcg tgcaggtgct gggcaacctg aagttcgatg
     1741 aggcaaggcc cgacgtgtcc gaggaccagt gccgggagct gcgaggaagg ctcgggttcg
     1801 acgaacgcga ccttgtgctg gtgttcggat cgaccgcgcc ggaggagaac gaggcgttac
     1861 tggacgcttt cgccgtcatc cgggccgagg aaccgcgcgc gcgcctcatt atggcaccgc
     1921 ggcacctcga gcgcgttccc gactgcgtcc gggcggcaga gcagcacgga ttccgcacga
     1981 tcttgaggtc acagctcccc gccgacggcg acaacgatcg cattgtcgtg ctcgacacct
     2041 tcggggagct ggccactctg tacgcgttga gcgccgttgc cttcatcggc cggagtctgg
     2101 tgcctctggg cggaggaaac ctgcttcagc ctcttggatt cggcgtgccg gtcgtgttcg
     2161 gacctcacat ggagaacttc cgagaggcgg ctcggtcggc gctcgaggcc ggcttggctt
     2221 tcgaggtggc gtcgtcgggc gacctggccg aggtctgctg ccgcttgctc cggtcaccgg
     2281 acgagcgcgc ccggattgcg gagcgggcac cggcatttct ggcggacaac gcgggggcgg
     2341 ccggccgcca tgctgaggct ttggcgggct tgcttggttg gtgtgggact gaggcacagt
     2401 gaacctggct atgcagtggg tcgagctggt ggaacagggg ccgcggaacg cccaaggccg
     2461 gctggcgcac ttgtgcctcc gcggcatgtc ggtgttctat ggcttggcac ttgatgccaa
     2521 cctcgagctc taccggcggg ccatactgaa gcgacaccca gcgggcctcc cggtgatcag
     2581 tatcggcaac ctcacactgg gtggcaccgg gaagacgacg ttcacgcaga tgctggcccg
     2641 gcagcttctg gatctcggtc agcgccccgc catcctcctg cgggcctaca agcgaggcct
     2701 ccgcgatcac tgcgacgagt acgaggtcta ccgggcagga ctgccggagg cggttcagat
     2761 cctcgagggc cggaaccgag tggcctcggc agcccgcgcg cggtccgaag gtgccaccgt
     2821 cgccctgttg gatgatgggc accagtatct gaagctctcc aaggacctcc gcatccttct
     2881 gttcgactgc actcagcccc ggcatctcga tcgcctgttc cccgcgggaa tgctgcgcca
     2941 gcctctgagc catgtcgcca ccgcggacct ggtaggcctg acacggtgcg accaggcggg
     3001 gccggaagca gtcgcccgga tgcgcgcccg gttgcggagc gcgggcgcgc aaggggattt
     3061 cgtcgagtgc gcccatcgcc ccacttgcct tgtcgacggg cgcaccggcg aacggctggg
     3121 ggcccgggaa ctggccggcc agcgcgtcgt ggcagtgagt ggcatcggga acaacgaggc
     3181 cttcggacgg actgtggcct cgctcggcgt tgaggtggcg cgacacctcc gtttccccga
     3241 ccaccacgcg ttctcagcgc gcgacgccga ccacatcacc gccgaggtgg agtcccgcca
     3301 agtgtcgtgt gtcgtgacga ccgcgaagga cgccccacgg ctggccccag tgttgcgccg
     3361 tgtcgacttc gtggctgtcc gggtcgagat ggagatcctc tcgggggccg agctgctgcg
     3421 ggcgcggctg tcgtccatcg ggctaacgcc tggagagcct ggtgcggccc atgcctgacc
     3481 gcgacctggc tgcccttcac gcctatgccg actggcggac ccgccggacg cgggcgctcc
     3541 tgccctggct gcggttgaag gcgctgacga ggggcccgca gggccgtcag cgcctgtccg
     3601 cagggctagg ccacgtgacg ccgggctgca tgtgccccag atcgtgccgg cacctggtcc
     3661 tgtactgcca caacatcggc gaggcgcgta ccgcgctgcc cctggtagtc gggctgcgga
     3721 agcgcgatcc catgctgcgc tacgtggtct gggccaagag cgtcgcggct ttcgaagcag
     3781 caccgggcat cctgcccgag ccgtgtgccg tgctgtacgc accactcgac gggacccagg
     3841 ctgtccggcg cgcgctggag aggtgccgcg ccttggccct cgtgatcctc gagggtgacc
     3901 tacgcccgac gatggactgg ggggcccgcg aggcaggcat ccccaccgct ttcgtgagtg
     3961 ccggcttatc gcccgtggag atggcccgcc accgggggca ccgggagggc cagaagcaga
     4021 tgctcgaggc cctcgacctc ttggccctca agagcgaggc cgacgcgcag cgcgccatcg
     4081 agttgggggt cgaccggggc cacgcctggg tctccggcaa ctgccgcttt gacgtgggac
     4141 gactggatct gacgccgcct cgaggaccac tcgtcgagtt catcgaaggg gctcgcctca
     4201 tcggacgagt ggtcatggtg gccgggagca catacgggcg ggaggaacgc tacctggcgg
     4261 aggttcgcag cgccccccgt ctcgccgggt ctctgctgct agtggttgcg ccccgcaagc
     4321 ccgggcgagc ggacgaagtg ctggcgacct tcaggcgcgc cggcgcgcgc gtcgcgcgcc
     4381 gcacggagct cggcaccggt tcggggcctc tcgatctgcc ggcggacgta ctggtgctcg
     4441 acaccatggg agagctgctc accacctatg gactcgccga cgtctgcttc gtcggcggga
     4501 gcctggtcaa caagggcggg cacaacctca tcgaggct
//
LOCUS       DAOSPL010000140         4494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_19398_length_4494_cov_4.064204, whole genome shotgun sequence.
ACCESSION   DAOSPL010000140 DAOSPL010000000
VERSION     DAOSPL010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19398_length_4494_cov_4.064204"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLQ54_03825"
     CDS             <1..409
                     /locus_tag="PLQ54_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92416.1"
                     /translation="RKMGWHFTNMLAPAGIGFAVLVFAWVLVGVPVVGVLVTLGLIAL
                     ACLQLGGALMTDMGRETRPTVFWPLRRFKRWRDGGRRFSGDPWQYTNGDDTDYEAQYD
                     LLAAQHGEGAPPPAPSQHRMRHPRHRSHPRRRS"
     gene            603..1919
                     /locus_tag="PLQ54_03830"
     CDS             603..1919
                     /locus_tag="PLQ54_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS92417.1"
                     /translation="MTADRRRGLSRREFVRRSVVLGGTVVLPYVVPGRALGKDGAVAP
                     SERVVVGAIGIGPRGKTVLDSFFAQPDVQFVAICDVRADRREAVKAAADAHHGSNDCV
                     MYRDLRDLLARDDIDAVLIATGDRWHSLGTILAVKSGKDVYSEKPCALTIGLCQSMAD
                     AVHRYGRVFQAGTQRRSIGNFQAAVRLAHSGKLGRLRTLHASSYRPRVTHEWLPEEEI
                     PPREVIDWDLWLGPAPWRPYNSAYVNGGWRGHFDFDSGCRLLDWGAHTVDLCQWANQS
                     DDTLPTDYEASEDKITARYANGVELVIDFLETPFGNRDPLYGTSRGTCPVRYEGDEGW
                     VETGDSGEIAVYPESLRAELDVPPPVAGTDSMSHVRNFIDCVKSGARPNANVDVMRRS
                     HIACHAAALSWQLGRKLSIDPATEMFINDDEANRLRFRAMREPWRV"
     gene            1946..3466
                     /locus_tag="PLQ54_03835"
     CDS             1946..3466
                     /locus_tag="PLQ54_03835"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS92418.1"
                     /translation="MITRSRTAIALSISMCLMIAGSVVAQNTAPAGSATVPELIAVLR
                     SDAAAFDKAKACQGLAARGDARAVAALVPLLAEEELACYARSALEAIADPSAGAALVE
                     ALGQLRGGLLVGAINSVGARREARGVDILSKLVTGDDPEAAAAALAALGRIGTPEAVR
                     VLRRALEQAPSRLRAEAADACLVCGDALLDRGDRGEAVGLYRRVRAARVPEHIRLAAL
                     GRQMVALGPRCVPILLRHLRSGDTPERTIALRAARDIPGPEVTRALARELPSAGPALQ
                     VQLIEALAGREGEAARSAIERLIADDVREVRLAALRAVGPVDVDRLRFVPLFDGRTFE
                     GWEGDTVGSFRVEDGAIVGGNLNTPIPRNEFLCTTRTYANFVLRLECKAVSANGGIQF
                     RSQRVPGSSEVSGYQADMDSSGTYWGCLYDESRRGMLVQADPAATSKVVKLDDWNHYE
                     IRCEGNHIQLLVNDLRTVDYVESDPTIPRSGVIGVQVHAGPASETWYRNIRIAELP"
     gene            3563..4198
                     /locus_tag="PLQ54_03840"
     CDS             3563..4198
                     /locus_tag="PLQ54_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOS92419.1"
                     /translation="MGLVEFVNNYEDLSTDKGFQFKFFCEHCGNGYMTQYKVSKLGVA
                     TGLLDAAGRLFGGALNRAADSGYEIQRSIGGPAHDAALREAVEEVKAHFKQCSRCGQW
                     VCPEHCWNASRGMCERCAPDLAEEIAAAQQEAAIEQVREKVREIDYTKDLDLGTPASA
                     TCPHCGAKTGPGKFCTECGKPLAARTECPQCGQELKAGARFCSECGARIGG"
     gene            4191..>4494
                     /locus_tag="PLQ54_03845"
     CDS             4191..>4494
                     /locus_tag="PLQ54_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92420.1"
                     /translation="MAEPTPLVLNGAYMIRAGSSVTAEGSAELRIGPDGVFIAPGAGP
                     PTGIPYTDMEAILCGEHVITVPLRGGQVLQLRRLGGRFDEAFLALRQCHVDALSVAL"
BASE COUNT          707 a         1425 c         1614 g          748 t
ORIGIN      
        1 ccgcaagatg ggctggcact tcacgaacat gctggctcca gcaggcatcg gcttcgccgt
       61 cctcgtcttc gcctgggtgc tggtgggcgt gccggtggtc ggcgtgctcg tcacgttggg
      121 cctgattgcc ctggcctgtc tgcagctcgg aggcgcgttg atgaccgaca tggggaggga
      181 gacccgtccg accgtcttct ggccattgcg ccgcttcaag cgctggaggg atggcggcag
      241 acgtttctcc ggcgacccct ggcagtacac caacggcgac gacacggact acgaagccca
      301 gtatgacttg ctggccgccc agcatggcga aggcgcgccg ccaccggcgc ccagccagca
      361 ccgcatgaga cacccccggc accggagcca cccgaggcgt cgaagctgag cccggacggg
      421 agcatcccgc ctccgccgcc tccgcctccg cctccgccgc cttctgatcc ggctggttag
      481 ggcagtccgg cgagaacggc gggccgcggc agtctgccgc gacccgccgt cgtgtaccgg
      541 aggtgaaagg gcggcagcgg cgtattggtc ccctggactc ggctaccagg gaggcacatg
      601 tcatgacagc ggatcgacgt cgcggtctga gccgtcgcga gtttgtccgg aggtcggtgg
      661 tcctcggggg caccgtggtc ctaccatatg tggtccccgg tcgagcgctg gggaaggacg
      721 gcgcggtagc gccctcggag cgcgtcgtgg ttggcgccat cggcatcgga ccgcggggca
      781 agaccgttct cgactcgttc ttcgcccagc ccgatgtgca gttcgtggcc atctgcgacg
      841 tacgggccga ccggcgtgag gcggtgaagg cagcggccga tgcccaccac gggagcaatg
      901 actgcgtcat gtaccgtgat ctacgggatc tcctggcgcg cgacgacatc gacgccgtgc
      961 tgatcgctac gggggaccga tggcactcgt tgggcaccat cttggcggtg aagtcgggca
     1021 aggatgtcta cagcgagaag ccatgcgcgc tgactatcgg cctgtgccag tccatggccg
     1081 atgcggtcca tcgctatggg cgtgtctttc aggccgggac tcaacgccgc agcatcggca
     1141 acttccaggc cgccgtgcgg ctggcccatt ccgggaaact gggcagactc cgcacactcc
     1201 acgcttcgag ctaccggccc cgcgtcacgc atgaatggct acccgaggag gaaatcccgc
     1261 cccgggaagt catcgactgg gacctgtggc tgggccctgc gccttggcgg ccctacaaca
     1321 gcgcgtatgt gaacggtggc tggcgcgggc acttcgactt cgactcgggc tgccgcctgc
     1381 tcgactgggg cgcgcatacc gtcgacctgt gccagtgggc caatcagtcc gatgacactc
     1441 ttcccaccga ctacgaggca tcggaggaca agatcacggc acgctacgcc aacggcgtcg
     1501 agctggtgat cgatttcctg gagaccccgt ttggcaaccg ggatccgctc tacgggacat
     1561 cgcgagggac gtgcccggtg cggtacgagg gtgatgaggg ctgggtggag acgggcgata
     1621 gcggggagat cgcggtgtac cccgagtcac tacgggccga gctcgacgtg ccgccgccgg
     1681 tggctggaac cgactcgatg agtcatgtgc ggaacttcat cgactgcgtg aagtcgggcg
     1741 cccgtcccaa tgccaatgtg gacgtgatgc gtcggtcgca tattgcatgc cacgccgcgg
     1801 cgctgtcgtg gcagctcggc cggaagctga gcatcgatcc ggctaccgag atgttcatca
     1861 acgacgacga ggccaaccgc ctgcggttcc gcgccatgcg cgagccgtgg cgggtctgac
     1921 gcgcgcgatt gctgaggagc ctgccgtgat cacccgctcg agaaccgcca tcgccctttc
     1981 gatatcgatg tgcctcatga tcgcggggtc ggtcgttgcg cagaacacgg ctccggccgg
     2041 ctctgccacc gtgccagagc tcatcgcggt gctccgctcg gacgccgcgg ccttcgacaa
     2101 ggccaaggcc tgccagggcc tggctgcgcg gggcgacgcc cgagccgtgg cagcgctcgt
     2161 gccgctgctt gccgaggagg aactcgcctg ctacgcccgg tcagctcttg aggcgattgc
     2221 cgatccgtcg gccggcgcgg cgctggtgga ggccctgggg cagctcagag gtggtctatt
     2281 ggtcggtgcc atcaactcgg ttggggcccg gagggaagcc cgaggggttg acatcctctc
     2341 gaagctggtg accggcgacg accccgaggc ggctgccgcc gcgctcgcgg cgttggggag
     2401 gatcgggacg cccgaggctg tgcgcgtcct gcggcgcgcg cttgagcagg ccccgtctcg
     2461 gctgcgtgcc gaggccgctg acgcctgcct tgtgtgcggt gatgcgcttc ttgatcgggg
     2521 tgaccgaggc gaggcggtgg ggctgtacag gcgcgtgcga gcggcccgtg ttccggagca
     2581 catccgcctc gccgccctgg gacggcagat ggtcgccctg ggtccgcggt gtgtgcccat
     2641 cctgctgcgg cacctccggt cgggcgacac gccggaacgc accatcgcgc tccgggccgc
     2701 gcgcgacatt ccgggccccg aggtcacgag agctctggcg cgcgagctcc catcggctgg
     2761 cccggcactc caggtgcagc tgatcgaggc gttggcgggg cgtgaggggg aggcggcgcg
     2821 gtcggccatc gagaggctta tcgccgatga cgtccgcgaa gtgaggttgg cggcattgcg
     2881 ggcggttggg ccggtcgatg tcgaccggct gcggttcgtc cccctgttcg atgggaggac
     2941 attcgagggc tgggaaggcg acacagtggg atcgtttcgc gtcgaggatg gcgccatcgt
     3001 cggcggtaac ctgaacacgc ccatcccacg caacgagttt ctgtgcacca cgcgcacgta
     3061 cgcgaacttc gtcctgcggc tcgagtgcaa ggcggtctcg gccaatggcg gcatccagtt
     3121 ccgctcccag cgggtaccgg gtagctcgga agtgagcggc taccaggctg acatggacag
     3181 cagtgggacg tactggggct gcctctacga cgagtcgcgc cgaggaatgc tggtccaggc
     3241 cgacccggcg gcgacgagca aggtcgtgaa gctggacgac tggaaccatt acgagattcg
     3301 ctgcgagggc aaccacatcc agctactagt caacgacctg cgcacggtcg actacgtgga
     3361 gtcagacccg accattcccc gaagtggtgt catcggtgtg caggtccatg ccggacccgc
     3421 gagtgagacc tggtaccgca acatccggat tgcggagttg ccctagcagg acggggcccg
     3481 cggtcccgtt gaggtgccca ggaggctgtg ttacgatcgt cgcgcctggg taatgcagga
     3541 gtgcggagga gggtcggttc gcgtgggcct ggtggagttc gtcaataact atgaagacct
     3601 cagcaccgat aaggggttcc agttcaagtt cttctgcgaa cactgtggca acggatacat
     3661 gacccagtac aaggttagta agctgggcgt ggcaacgggc ctgctcgacg cggcgggacg
     3721 gctctttggc ggtgccctga accgggccgc ggatagtggc tatgagatcc agcggtccat
     3781 cggcggaccg gctcacgatg cggcgcttcg cgaggcggtt gaggaagtca aggcccactt
     3841 caagcagtgc tcgcgctgcg ggcagtgggt gtgccccgag cattgctgga acgcgagccg
     3901 cggcatgtgc gagcgctgtg ctcccgacct ggctgaggag atcgcggcgg cccagcagga
     3961 ggccgctatc gagcaggtcc gcgagaaggt ccgcgagatc gactacacca aggacctgga
     4021 cctgggcacg cccgccagcg ccacgtgtcc ccactgcggg gccaagacgg ggccggggaa
     4081 gttctgcact gagtgcggga agcctctcgc ggccagaacc gagtgcccac agtgtgggca
     4141 ggagctcaag gctggggcgc gcttctgctc ggagtgcggg gcacggatcg gtggctgagc
     4201 cgacgccgct ggtgctgaat ggcgcgtaca tgatccgcgc cggaagcagc gtgactgccg
     4261 agggttcagc ggagctacgg atcggcccgg acggcgtgtt catagcgccg ggtgcgggtc
     4321 cgccgaccgg catcccctac acggacatgg aagccatctt gtgtggcgag cacgtgatca
     4381 ccgtgccgct ccggggggga caggtgctcc aactgcgcag actcggtggc cgtttcgacg
     4441 aggcctttct cgccctccgg cagtgccacg tcgatgcgct gtccgtggca ctgc
//
LOCUS       DAOSPL010000141         4488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_19449_length_4488_cov_3.147304, whole genome shotgun sequence.
ACCESSION   DAOSPL010000141 DAOSPL010000000
VERSION     DAOSPL010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19449_length_4488_cov_3.147304"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..803)
                     /locus_tag="PLQ54_03850"
     CDS             complement(261..803)
                     /locus_tag="PLQ54_03850"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOS92421.1"
                     /translation="MSTCDECGTSPATVSVCMTINDVRFEVNLCDACARRRAEEGAIT
                     LGFVLDEAAAEPAGTRREPLVDAAAFSSVCPVCGLTAEAFSQSELLGCDTCYTAHAGI
                     LRAVLRTVQGVDVPLTEARASRGHSTRAAKMRQLTAELDRAIELEEYLRAAQLRDEIR
                     ALREPAARGRTRRARPTADE"
     gene            complement(812..1705)
                     /gene="ilvE"
                     /locus_tag="PLQ54_03855"
     CDS             complement(812..1705)
                     /gene="ilvE"
                     /locus_tag="PLQ54_03855"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOS92422.1"
                     /translation="MVYLDGRIVPAGEAMVSVYDHGLLYGDGVFEGIRVYHGRVFRLE
                     QHIRRLYRSARAIMLDIPLTPSELIDATLATLRAGECTDGYIRLIVTRGVGDLGLDPG
                     KCPKASIIIICDGISLYPAELYERGLEVITCATRRTPPTSLDPSVKSLNYLNNILAKI
                     EVKRAGLLEGIMLNSEGYVAEATGDNVFVVSEGQLLTPPTHCGILVGVTRDAVMELAR
                     REGIEVQERVLTPVDLYAADECFLTGTAAEVIAVVKIDGRVIGDGAPGPVTKRLRVAF
                     HALTETEGVPIYPEGAAGSSG"
     gene            1913..2701
                     /locus_tag="PLQ54_03860"
     CDS             1913..2701
                     /locus_tag="PLQ54_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS92423.1"
                     /translation="MSIRVEEIQCKSALTGSGGRFRLNPYCGCEHSCAYCYATYLTRW
                     RGQSAPWGSWVQVKTNIARVLECELARRRVAHVMLSTACDAYQPVEEHYRLTRRCLSV
                     LAIASQKLDGPSVFVLTKSDRVLGDLDVLRMFPEGSLRVAFSVTTHRDDVAAILEPGA
                     PSPSARIAAATALVAEGICVELFINPVLPYITERDMPALLDAARVAGCGVGGFDRTNY
                     LDRQPGARLRAVYRHLGPEAQERLEQARNERRSEVPTSWSPGGY"
     gene            2721..3509
                     /locus_tag="PLQ54_03865"
     CDS             2721..3509
                     /locus_tag="PLQ54_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92424.1"
                     /translation="MPVFQIEDRTFTRLILGHNPLLGYSYLSHARCREYAERFATYEP
                     IRDVIVAALRMGVRSIMISPGGEQSDRVAQAIEAAQEHTGITMSNLVIVGPDIPSQMD
                     YLKRVGCQVCLVHGQVTDSMFWRAEWTFRPEFAELLAAIRAAGFVPGMSSHNGGETVP
                     VAERFDVQVINTPVNKIAWRMCPCVEQVLEAVRRSTRKIIAMKPLAMGRIAPQEALEY
                     ALQVPGVDALCIGIATPAEAEETFAIGAEVLARTGCTNGRGASA"
     gene            3655..>4488
                     /locus_tag="PLQ54_03870"
     CDS             3655..>4488
                     /locus_tag="PLQ54_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS92425.1"
                     /translation="MGRVRAVVVGAGGIAGAWFPPLLKEDVEIAAVVDLNREAARRRV
                     EEYAPSAEATTDLSTALASHAPDFVVDLTVPEAHCEVTCTALEAGCHVIGEKPMASRM
                     DEARQMVRASERTGKLYMVSQSRRWNPAHEQVRRVVAEGALGTVTTVNCDFYIGAHFG
                     GFRDEMPSPLILDMAIHHFDLARYMTGEDATHVYAREFAPAGSWYRGDAAASCVFEMT
                     HGVVFTYRGSWCAEGCHTSWNGDWRIVGERGTVVYERDQEPRGQVVAGDEGFHRPLQD
                     LR"
BASE COUNT          696 a         1495 c         1537 g          760 t
ORIGIN      
        1 gtatgggagc ggggcattgg cggtcgcttg gggcgccagc ggcccatggt ccgggtcgcg
       61 gcggggcgcc atgggggccg ctggccgctt gggctgaccg tcccggccgg ctatgagatg
      121 ccgagcgggc cgctcgaccc acgcgcgttt cgggcgtacc cctgtgcggg tcctccacca
      181 gacgggtcag ggcgtcagcg cagcgtacgg gcctttcgcg caccgccccg gcccaggcta
      241 ccgcccatgc gggagcaggg ctactcgtcc gcggtcgggc gtgcgcggcg ggtgcgtccg
      301 cgggcagctg gctcgcggag cgcgcggatc tcgtcgcgca gctgggccgc tcggaggtac
      361 tcctcgagct caatggcccg gtcgagctcc gccgtcaact ggcgcatctt ggcggcgcgt
      421 gtcgagtggc ctcgcgaggc gcgcgcctct gtcagtggta cgtcaacgcc ctgtaccgtc
      481 cgaagcacgg cgcggaggat gccggcatgg gccgtgtagc aggtgtcgca gccgagcagc
      541 tcactctgcg agaacgcctc ggcggtgagc ccacacacgg ggcacacgga cgagaatgcg
      601 gccgcgtcga ccaggggctc gcgacgtgtg ccggctggtt cggcggcggc ctcgtcgaga
      661 acgaatccca gtgtgatggc gccctcctcg gctcgacggc gcgcacaggc atcgcacagg
      721 ttcacttcga accgcacatc gttgatggtc atgcacacgg agaccgtcgc cggactggtc
      781 ccacactcgt cacacgtgct catatcgatc tctagccgga tgacccggcc gctccctcgg
      841 gatagatcgg taccccttcg gtctcggtga gggcgtggaa cgcgactcgt agtcttttcg
      901 tgactggccc cggcgctcca tcgccgatca cgcggccatc gatcttgacc accgcgatca
      961 cctcggcggc cgtgccagtg aggaagcact cgtcggcggc atagaggtcc acgggtgtca
     1021 ggacccgctc ctggacctcg atgccctccc ggcgggccaa ctccatcaca gcgtcgcgcg
     1081 tcacgccgac caggatgccg cagtgcgtcg gtggcgtcag cagttgcccc tcactcacta
     1141 cgaacacatt gtcgcctgtg gcctcggcca catagccctc ggagttgagc atgatgcctt
     1201 cgagtagtcc cgcgcgcttc acttcgatct tggcaaggat gttgttgagg tagttcagcg
     1261 actttacgct cgggtccagc gatgtgggcg gcgtccgcct tgtggcacag gtgatgacct
     1321 cgagacctcg ctcatacagc tcggcggggt acagcgagat cccgtcgcag atgatgatga
     1381 tcgatgcctt cgggcacttg ccggggtcga gccccaagtc gcccacgcca cgtgtgacta
     1441 tcagccggat gtacccatcc gtgcactcgc ccgctcgcaa cgtcgcgagg gtcgcatcga
     1501 tcagctcgga gggtgtgagt gggatatcca gcatgatggc gcgggccgag cggtatagcc
     1561 gacggatgtg ttgctccagg cggaacacgc ggccatggta gacacggatg ccctcgaaaa
     1621 cgccatcgcc atagagcagg ccgtgatcgt agaccgagac catggcctcc cccgcgggca
     1681 cgatccggcc gtcgagatac accatcggtc tcacacgcat ctacctcgcc gctctacaga
     1741 gggagctgta tccgaacgcg cctgcgccgg cctcacccgg gaggcacctt cgcgccacga
     1801 tagcacaatg cggtgtgagt ggcaacgcga gtcccgtgcg cgttgcagga cggctcgggg
     1861 gcgcaggatt ggccggtgtc tgctcgaaac ccacttgggg gcgctgcccg gcatgagcat
     1921 ccgcgtcgaa gagatccagt gcaagtccgc cctcaccggt tcgggcggtc gcttccgcct
     1981 gaacccctac tgcgggtgcg agcactcatg cgcgtactgc tacgccactt acctgactcg
     2041 ctggcgcggc cagtcggccc cctggggctc gtgggtgcag gtcaagacga acatcgcaag
     2101 ggtgctcgag tgcgagctgg ctcgccggag ggtcgcccat gtcatgctct cgacggcctg
     2161 cgatgcctat cagcccgtcg aggagcacta ccgcctgacg cgccggtgcc tgtcggtgct
     2221 cgccatcgcc tcacaaaagc tcgacgggcc cagcgtgttc gtgctcacga agtccgaccg
     2281 ggtcctgggc gatctggatg tgcttcgcat gttccccgag ggcagcctca gggtcgcttt
     2341 ctcagtgacc acgcatcgcg atgatgtcgc ggccattctc gaacccggcg cgccctcccc
     2401 atcggcccgc atcgcggcgg cgacggctct ggtcgccgag ggaatctgcg tcgagctgtt
     2461 catcaacccc gtgctcccct acatcaccga gcgggacatg ccggcgctcc tcgatgcggc
     2521 ccgagttgcg ggctgtggcg tcggaggctt cgacaggacg aactacctcg accgccagcc
     2581 cggcgcccga ctgcgtgccg tctatcgtca cctgggcccc gaagcccagg agcggctcga
     2641 gcaggcccgg aatgaacgcc gaagcgaggt cccgacctcg tggtcgccag ggggctactg
     2701 aaatgcagga gtgcgccaca gtgcccgtct tccagatcga ggaccgcact ttcacgaggc
     2761 tgatcctcgg ccacaaccca cttctcggct actcctacct gagccatgcc cgctgccggg
     2821 agtacgcgga gcgcttcgcg acctatgagc ccatccgtga tgtcatcgtg gccgcgctcc
     2881 gcatgggcgt ccgaagcatc atgatcagcc ccggcggcga gcagtccgac cgtgtggccc
     2941 aggccatcga ggccgcgcag gagcacacgg gcatcaccat gtcgaacctg gtcatcgtcg
     3001 gcccggacat ccccagccag atggactacc tcaagcgcgt aggctgccag gtgtgcctcg
     3061 tccacggtca ggtgaccgac tcgatgttct ggcgcgcgga gtggacgttt cggcccgagt
     3121 tcgccgagct cctggccgcc attcgcgctg cggggttcgt gccggggatg tcgagccaca
     3181 acggcggcga gacggtcccc gtggcggagc ggttcgatgt gcaggtcatc aacacgccgg
     3241 tcaacaagat cgcctggcgg atgtgcccct gtgtcgagca ggtgctcgag gccgtccgaa
     3301 gatcgacgcg caagatcatc gctatgaaac ctctggccat ggggcgcatc gctccgcagg
     3361 aggccctgga gtatgccctc caggtccccg gtgtggatgc tctgtgcatt ggcattgcca
     3421 cccctgcgga ggctgaggag accttcgcca tcggcgccga ggtgttggcc cgcaccggct
     3481 gcacgaacgg acgcggcgcg tcggcgtgat ggagctgtgg cccgagggtt gcggcgtgcg
     3541 gccggatcgc caccatggcg tccggctccg tttacgttcg aagcgcgtgc ggggtacact
     3601 ccatccatgc gcggccggca gcgaggtgtg gggccgccac gggagggctg cttcgtggga
     3661 cgcgtacgag ccgtggttgt gggtgcgggc ggtatcgcag gggcgtggtt cccgccgctg
     3721 ctgaaagagg atgttgagat cgccgcggtg gtggacctga accgcgaggc cgcccggcgt
     3781 cgcgtcgagg agtacgcccc ctcggccgag gccactacgg acctgtccac cgcactggcg
     3841 agccatgcac cggacttcgt cgtggatctg accgtgcccg aagcacactg cgaggtgacc
     3901 tgcacggcgc tggaggccgg gtgtcacgtc attggcgaga agccgatggc ctcgcggatg
     3961 gacgaagcgc gccagatggt gcgggcttcg gagcgcacgg gcaagctgta catggtgagt
     4021 cagtcgcgcc ggtggaaccc ggctcacgag caggtgcgac gggtcgtggc cgagggtgcc
     4081 ctgggaaccg tgacgacggt caactgcgac ttctacatcg gcgctcactt tggcgggttc
     4141 cgcgatgaga tgcccagccc gctcatcctc gacatggcca tccaccattt cgatctggcg
     4201 cgctacatga ctggcgagga cgccacacac gtgtatgcgc gcgagtttgc ccccgccggc
     4261 tcgtggtacc gcggtgacgc cgcggcgagc tgcgttttcg agatgacgca cggcgtcgtg
     4321 ttcacctacc gcggcagctg gtgcgccgag ggctgccaca cgagctggaa tggcgactgg
     4381 cgcatcgtcg gcgaacgcgg tacggtggtc tatgagcggg accaggagcc tcgcggccag
     4441 gtcgtagccg gcgacgaagg gttccatcgg ccgcttcagg atctgcgt
//
LOCUS       DAOSPL010000142         4481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_19480_length_4481_cov_3.332806, whole genome shotgun sequence.
ACCESSION   DAOSPL010000142 DAOSPL010000000
VERSION     DAOSPL010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19480_length_4481_cov_3.332806"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLQ54_03875"
     CDS             complement(<1..313)
                     /locus_tag="PLQ54_03875"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOS92426.1"
                     /translation="MRASRITAEVIRCGFTESVHDIIACVRDAEGKVVLAAGDISRPA
                     MPRSSLKPLQALAPQAAGVVDAFGLTQREAAIFCGSHHGESFHVEAVESILRKIGLDG
                     SA"
     gene            complement(396..4466)
                     /gene="uvrA"
                     /locus_tag="PLQ54_03880"
     CDS             complement(396..4466)
                     /gene="uvrA"
                     /locus_tag="PLQ54_03880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOS92427.1"
                     /translation="MASGPPSAIAESPESITGAYLRGARTAIRLGPRRPAVGPAITIR
                     GARFRNLKNLDVRIPLGALVCVTGVSGSGKSTLVHGILRPALSRLLTQGRSSRPVGDH
                     DAVEGAEQIARVIEIDQQPIGRSPRSNPATYTRAFDHIRNAFAKMPEAQVRGFGLSHF
                     SFNVAGGRCEACSGAGVIRVPMQLVPDVFVECEECGGKRFSRDVLRVRYRGVSISQVL
                     EMTAEAARELFSSIPAVERAMSALCEVGLDYIRLGQPATSLSGGEAQRVKLAAHVAKP
                     RMGHTLYLLDEPTTGLHFADIDRLLHLLGRLVDEGNTVLVIEHNPDVIRSADWVIDLG
                     PEGGDGGGEIVAEGTPEDLQALGRRGDCRSWTARLLASPDDCIADAPGPRMWPAGGPQ
                     RQLCVRGARANNLQAIDVDIPKAAITCVTGPSGSGKSSLVFDTIYAACQNTFAQCLSS
                     YARQALGGMSALDATSVTGVSPAVAVDRRSRVASSRSMVGTATEVLQLLRVLWARVGV
                     RHCPQCGEAVRPLTADAIADRIAADAGGRMIQLVAPVARGARRRREVAELVADLVAGG
                     LVRVRLNGRVFNLSVEELDFGTKRRHDVDCIVDRLKPSRERLDRLAESVRLALDLGAG
                     GLVCEFVDGEGLGPATTYSSRIQCAQCRASLEPELTARHLSFGHPLGQCPQCRGTGTV
                     RAFDPGSIVPDPSRSFARGAIAPLTRAQYADPRSDWSRAFAALGRSFGFDRNTPFADL
                     DARQLRVVLYGAGDRRLSIHVNGPSGSVVRRTWKGLIPILEGAIRRDPRKAKGLARYQ
                     ADVRCSACDGERLMPEFRAVRVGGRRLPELVEMTVDEALEFVSTLTLPDAQQQTASGL
                     LSELRGRLRFMQGVGLGYVTLSRGMGTLSAGESQRVRLAAQLGGTLSGLLYVLDEPSV
                     GLHPHDMVSLVFALEELRELGNTVIAVEHDEAMMAHADYLVDMGPGPGRLGGRVMAAG
                     PRDDVLPAVLAARPALRSGSCVPASRQRCIRLTGVRHHNLRNVTVHVPIGALTCVTGV
                     SGSGKSSLVFDVLRAALRRRLQGHGPAPGEHDSIEGLAAIDHVIVVTQAALGQSMRSV
                     PATYVGFFDAVRGLFASLPESRARGLTRAHFSFNDAKGRCRACQGDGYRQVDLQLMAD
                     LTFVCEQCQGRRYQDEVLTVTYQGKSIADVLALTFDEAAVLFQGVPEIEAPIAAVCRA
                     GLGYVVLGQPSPTLSGGESQRLRLATALVRPSSGHGLYLMDEPTTGLHARDVAVLHRV
                     LRQLVDSGATVVIIEHNLDMIAAADHVIDLGPGGGPNGGRLLVEGPPEAVMGCRESVT
                     GMYLRTHVGHTAEGVRNGSPSRRRSSSRREGTAGEG"
BASE COUNT          789 a         1619 c         1475 g          598 t
ORIGIN      
        1 tggcggatcc gtccaggccg atcttccgga ggatggactc cacggcctcg acgtggaaac
       61 tctcgccatg gtgcgagccg caaaagatgg cggcctcgcg ctgagtgagg ccgaaggcgt
      121 cgacgacgcc ggccgcctgc ggggccagag cctgcagggg cttcagcgac gaccgcggca
      181 tggcaggccg agagatatcg ccggcggcga ggacgacctt cccctcggcg tcacggacgc
      241 acgcgatgat gtcgtgcaca ctctcggtga acccacagcg gatgacttcg gcggtgatgc
      301 gcgacgctct catgtgtggt gcctcctcga tgtaccggat gagccacagg ccgatcgcaa
      361 cagcccgggc caacgggccc gcacctcatg cacggctatc cctcaccagc cgtcccctca
      421 cgccgggacg aagaccggcg gcgcgacggg gagccattgc ggacaccttc ggcagtgtgc
      481 ccaacgtggg tgcggaggta catgcccgtg accgactcgc ggcagcccat gacggcctcc
      541 ggtggccctt ctaccagcag ccgccccccg tttgggccgc cgcctggtcc caggtcgata
      601 acgtggtccg ccgcggcgat catatcgagg ttgtgctcga tgatcacgac ggtggcaccc
      661 gaatcaacca gctggcgcag cacgcgatgg agcacggcta cgtcacgggc atgcaggccc
      721 gtggtgggct cgtccatcag atagagtcca tgccccgacg agggcctgac gagggcggtg
      781 gccagcctca gccgctgcga ctcgccgccg gagagcgtcg gcgagggctg ccccagcacc
      841 acatagccca ggcccgcgcg gcacacggcg gcaatggggg cctcgatctc cggcacgccc
      901 tggaataaca ccgcggcctc gtcgaaggtc agggccagca cgtcagcgat ggacttgccc
      961 tggtacgtga ccgtcagcac ctcatcctgg tagcggcgtc cctggcactg ctcgcacacg
     1021 aaggtcaggt cggccatgag ctggagatcc acctggcggt acccgtcgcc ctggcaggcg
     1081 cggcaccggc ccttggcgtc attgaacgag aagtgggcac gcgtcagccc ccgtgcccgg
     1141 gattcgggca ggctggcgaa caggccacgt accgcgtcga agaaccccac ataggtggcc
     1201 ggcacggagc gcatgctctg acccagagcg gcctgcgtca cgacgataac gtggtcgatg
     1261 gccgccagtc cctcgatgct gtcatgctcc cccggcgccg gaccgtggcc ctggagtctg
     1321 cggcgcaggg cggcgcggag cacgtcaaag accagcgagc tcttgcccga cccggacacc
     1381 ccggtcacgc aggtgagcgc tccgatcggc acgtggaccg tcacgttccg cagattgtga
     1441 tggcggacgc ccgtgagccg gatgcaacgc tgccgggatg cgggaacgca cgacccgctc
     1501 cgcagggccg gacgggccgc cagtacagcg ggcagcacgt catcgcgggg gcccgccgcc
     1561 atgacccgcc ctcccagacg cccgggcccc gggcccatgt ccaccagata gtcggcatga
     1621 gccatcatgg cctcatcgtg ctcgaccgcg atgaccgtgt tcccaagctc cctgagctcc
     1681 tcgagggcga acacgagcga gaccatgtca tgcgggtgca acccgaccga tggctcatcg
     1741 aggacgtaca gcagccccga cagcgtcccg ccgagctgtg ccgccagtcg cacgcgctgc
     1801 gattcccccg cggacagagt gcccatgccc cgactgagcg tcacataccc cagtcccacg
     1861 ccctgcatga aacgcagccg gccccgtagc tcggagagca ggcccgatgc cgtctgctgc
     1921 tgcgcatcgg gaagtgtgag ggtcgaaacg aactcgagcg cctcgtcaac ggtcatctcc
     1981 accagctcgg gaaggcgtcg gccgcccacg cggaccgccc ggaactcggg catcagacgc
     2041 tccccgtcgc aggccgaaca gcgcacatcg gcctggtagc gggccagccc cttggccttg
     2101 cgggggtcac gccgaatcgc gccctcgaga atcggtatga gccccttcca ggtgcggcgt
     2161 accaccgagc ccgacggacc gttcacatgg atcgagagtc gccgatcgcc ggcgccatac
     2221 aagacgacgc gcagctgccg ggcatccagg tcggcgaagg gcgtgttccg gtcgaatccg
     2281 aaggatcgac ccagtgccgc gaaggcccgc gaccagtccg aacgcggatc ggcatactga
     2341 gctcgcgtca atggcgcgat ggcaccccgg gcgaaactcc gagacgggtc ggggacgatg
     2401 gaccccgggt cgaaggcccg caccgtcccc gtgccccggc actgggggca ctgcccgaga
     2461 gggtggccaa acgataggtg gcgcgcggtg agctcgggtt cgagcgaagc ccggcactgg
     2521 gcacactgga tcctcgacga gtaggtggtg gccgggccga gaccctcacc gtccacgaac
     2581 tcgcagacca gcccgcccgc gcccaagtcc agggcgaggc gaacgctctc cgccagacgg
     2641 tccagtcgct cgcgcgacgg cttcagacga tcgacaatgc agtcgacatc gtgccggcgc
     2701 ttcgtgccga agtcgagctc ctcaaccgac aggttgaaca cgcgcccgtt gagccgaaca
     2761 cgcaccaacc cgccggctac cagatcagcg acgagctcgg ccacctcgcg acgccgccgg
     2821 gccccgcgag ccaccggagc caccagctgg atcatgcgcc ctccggcgtc ggcggcaatc
     2881 cgatcggcaa tggcatcggc cgtcagcgga cgaaccgctt ccccgcactg cgggcagtga
     2941 cggacgccca cgcgggccca aagcacccgc agcagctgaa ggacctcggt agccgtcccc
     3001 accatggagc gcgacgacgc gacccggctg cgccggtcca cggccaccgc gggactgacg
     3061 ccggtcaccg atgtggcatc aagagcgctc atgccgccca gagcctgccg cgcatacgaa
     3121 gacaaacact gcgcgaaggt gttctggcat gcggcataga tggtgtcgaa caccagcgat
     3181 gacttgcccg agcccgaagg accggtgaca caggtaatag cggccttggg gatgtccaca
     3241 tcgatcgcct gcaggttgtt ggcccgagcg ccgcgcacgc acagctgccg ctgcgggccg
     3301 ccggccggcc acatccgcgg acccggagca tcggcgatgc aatcatcggg tgaggccaag
     3361 agacgagccg tccacgagcg gcagtcgcca cggcgcccca gcgcctggag gtcctcgggc
     3421 gtgccctcgg ctacaatctc gccgccgcca tcgcctccct cgggacccag gtcgatcacc
     3481 caatcggccg accggatcac atcggggttg tgctcgatga ccaacaccgt gttgccctcg
     3541 tcgaccagcc gacccaggag gtgaagcagc cggtcgatgt cggcaaagtg aagacccgtg
     3601 gtcggctcgt ccagcaggta cagcgtatgc cccatgcgcg gcttggccac gtgggcagcc
     3661 aacttcacgc gctgggcctc accgcccgag agactcgtgg ccggctggcc gaggcggatg
     3721 tagtcgagtc ccacctcgca cagagccgac atggcccgct ccacggccgg gatgctcgag
     3781 aagagctcac gagcggcctc ggcggtcatc tcgagcacct ggctgatact gaccccgcgg
     3841 tagcgcacgc ggagcacatc gcgcgagaag cgcttcccgc cgcactcctc gcactccacg
     3901 aagacatcgg gtaccaactg catgggcacg cgtatgaccc cggcacccga acacgcttcg
     3961 caccgcccgc cggccacgtt gaaactgaag tggctcaggc cgaacccgcg gacctgcgct
     4021 tcgggcatct tcgcgaacgc gttccgaatg tgatcgaagg cacgggtgta cgtcgccggg
     4081 ttcgagcggg gagatcggcc gatgggctgc tggtcgatct cgatgacccg ggcgatctgc
     4141 tccgcgccct ccaccgcgtc gtggtcgccc acgggtctgc tcgagcgtcc ctgggtcagc
     4201 aagcgagaca gcgccggccg caggatccca tgaaccagcg tgctcttccc cgacccggac
     4261 accccggtaa cacacaccag cgcgcccagc ggtatccgga catccaggtt cttgaggttc
     4321 cgaaagcgtg cgccgcggat ggtgatggcc ggccccacgg ccgggcgtct cgggccgagc
     4381 cgaatcgccg tacgcgcgcc gcgcaagtag gcgcccgtga tggactccgg cgactcggcg
     4441 atggccgacg gcggccccga tgccaccact cgcccgcccc t
//
LOCUS       DAOSPL010000143         4465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_19567_length_4465_cov_3.118141, whole genome shotgun sequence.
ACCESSION   DAOSPL010000143 DAOSPL010000000
VERSION     DAOSPL010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19567_length_4465_cov_3.118141"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PLQ54_03885"
     CDS             <1..683
                     /locus_tag="PLQ54_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOS92428.1"
                     /translation="MAAMLAAVDDAVGAMLAALERHGRRGNTFVYFQSDNGPSRESRN
                     WLDDTMTPYQGGTAGALRGHKFSLFEGGIRVPALCSWPARIPAGQVIEQPGAAMDVFP
                     TFLRAAGGDPAAYKLDGRDFLPLVTDGAASAHEALFLEMDQQTAVRRGPWKLVLNGQL
                     VEDEPPAAEVHLANLEDDPGERTNLAERLPELAAELRQQALQWREGVEAHWRSTWQPM
                     ARPATGHG"
     gene            complement(724..3246)
                     /locus_tag="PLQ54_03890"
     CDS             complement(724..3246)
                     /locus_tag="PLQ54_03890"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOS92429.1"
                     /translation="MPDPNTTVHRISLDGAGWWIQSRLPNEWIWAKAQDLEPGGPGWR
                     PATVPGSVQDDLLDQGVIPDPYYELQSRACEWTWDRDWIYARVLEVPEELRQGSPARW
                     TLVFHGVDYHAHVHLNGQKLAEHEGAFIPFEVDVTEQLRADAPNRLTVVVDRAPDEQW
                     QIGWTSRVRTWKPRFAYGWDWCTRLVPLGLWGSVELVRDTGLRITGLWARPVMSDDLG
                     SAQVTVEVAYSASRAASGAARVVIRGGEGIVTEATLPFEAQPGEHTVTATLSVDHPQL
                     WWPNGHGDQPLYTVCAWIQIPGAADERSTRIGFRRVRMITNDSAPAGALPYVIEVNGR
                     RIFIKGWNWAPIHHLYRRQSPEKYHRWLELVKEAHVTLLRVWGGGLLERELFYDLCDE
                     AGIMVWQSFCQSSSGIDNEPPRDSDYLNMAVRHAEAMIPQRRNHASLVLWCGGNELMH
                     ADWTPLDSKHPALAVLKDCVERLDPDRVYLPTSASGPSANASLERLGKMHDVHGPWTY
                     GGLYDHYRLYNGMDALLHSEFGVEGAARLRAYPRFVSAERVWPPDATNEIWTHHGAWW
                     INADRLGAMFGTLHDLPTHVRASQFIQAEGLRYAIEANRRRKWRCSGTSPWQYNESWP
                     NASCTNSLDWLGQPRPAYFWVRKAYEPLHASARYDTIARHEQRRVACTLFVSNSLADA
                     APVELVARLHGMDGALLSERVLRVTAEPCACTQGGEFEWEPDPSFADVALLRLTLRTR
                     DGLETTPNEYLFTWAPAPELSPLLSLPATRVELTPTGQGITLHNGGGAVALLVWVEPE
                     DDAEWPVFGDNYVCLLPGETRDIAVRQAPAAYVVDGLNLVKG"
     gene            complement(3300..>4465)
                     /locus_tag="PLQ54_03895"
     CDS             complement(3300..>4465)
                     /locus_tag="PLQ54_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92430.1"
                     /translation="ALPMVWDYVETNPLSYSVGSFAHHIEWVADGVAGLPARTPAGQA
                     YVADATAPRPPGTRAVICTDPPYYDNIEYADLSEFFLVWLQRSLGETLIRLCGTDQAS
                     KANELVASPFRDLPDAHSHFERGMAEALARLREAHDDRFPLVLYYGLKPAAASGGASG
                     ASAGWEAMLGAIVGAGLRITAAWPLRSERQSRSLAYGTNALASAVILACRPRQDGAPA
                     TTWDQCHEALMREVPLALEALVRAGTPPVDLAQAAIGPGMAVFTAHSRVTREDGQPVA
                     IGDVLAAIAEAVDAYLRSTVEGIAGDADLRVLTLSLGQQVVAGGPVAAAEGYTAAGCP
                     AEEGVIALAYWLYREAERSGALREAHAHNALAVAWPEIRRLAQPGPRGLGGER"
BASE COUNT          685 a         1490 c         1601 g          689 t
ORIGIN      
        1 tcatggcggc catgctggcg gccgtcgatg acgccgtcgg cgccatgctc gccgcgctgg
       61 agcgccacgg gcggcgcggg aacacattcg tctacttcca gagcgacaac ggcccctcgc
      121 gcgagtcccg gaactggctc gatgacacga tgacgccata ccaaggcggc accgcaggcg
      181 cgctgcgggg acacaagttc agcctcttcg aggggggcat ccgcgtgccg gcgctgtgct
      241 cctggccggc ccggatcccc gcgggtcagg tcatcgagca gccgggcgcg gcgatggacg
      301 tgttccccac gttcctgcga gccgccggag gagacccggc ggcctacaag ctcgacgggc
      361 gcgacttcct gccgctggtc acggacggag ccgcctcggc gcacgaagcg ctcttcctcg
      421 agatggacca gcagaccgcc gtgcggcgcg gtccatggaa gctggtgctc aacggccagc
      481 tcgtcgaaga cgagcccccc gcagccgaag tgcacctggc gaacctggag gacgacccgg
      541 gcgagcggac caacctggct gaacggctgc ctgagctggc tgcagagctt cgccagcagg
      601 ctctgcagtg gcgcgagggc gtggaggccc actggcgcag cacgtggcag cccatggccc
      661 ggccggcaac gggccatggg tagcggcgag ggacgagaga gcagggggtg gcccgcgcct
      721 gccctaccct ttcaccaggt tcagaccatc gacgacatat gctgccggag cctggcgaac
      781 agcgatgtcg cgggtctctc ccggcaacag gcacacatag ttgtcgccaa acacgggcca
      841 ctcggcatcg tcctcaggct cgacccacac cagtagcgcc acggcgccgc cgccattgtg
      901 cagagtgatg ccctgaccgg tcggtgtgag ctcgactcgt gtggctggca gactcagcag
      961 cggcgatagc tctggcgcgg gagcccaggt gaacaggtac tcgtttgggg tggtctcgag
     1021 gccatcgcgg gttcggagtg tcagccgcag caacgcgaca tcggcgaagc tcgggtcggg
     1081 ctcccactcg aactcccctc cctgggtgca agcacagggc tcggcggtca ccctcaggac
     1141 gcgctcggac agcagggccc catccatgcc gtggagccgt gcgaccaact cgacgggcgc
     1201 ggcatcggct agcgagttcg agacgaacaa ggtgcaggcg acccttcgct gttcgtgccg
     1261 ggcgatggtg tcgtagcggg ccgatgcgtg aaggggttcg taggctttcc tgacccagaa
     1321 gtaggcgggc cgaggctggc cgagccagtc caggctgttg gtgcacgacg cgttgggcca
     1381 gctctcgttg tactgccagg gcgaggtacc gctgcagcgc cactttcgcc gccggttggc
     1441 ctcgatggcg taacggagtc cctcggcctg gatgaactgg ctcgcgcgca catgggtggg
     1501 gaggtcgtgc agggttccaa acatggcacc gaggcgatcc gcgttgatcc accatgcgcc
     1561 gtgatgggtc cagatctcgt tggtggcatc ggggggccac acgcgttcgg ccgagacgaa
     1621 gcgcgggtag gccctgaggc gcgcggcgcc ctcaacgccg aactcgctgt gcagcagggc
     1681 atccatgccg ttgtacagcc tgtagtggtc gtagagcccg ccgtaggtcc acggcccgtg
     1741 cacatcgtgc atcttcccca ggcgttccag cgaggcgttg gccgatgggc cgctggcgct
     1801 ggtgggcagg tagacgcggt ctgggtcgag tcgctccacg cagtccttga gtacggccag
     1861 ggccgggtgc ttgctgtcca gtggggtcca gtcggcatgc atcagctcgt tgccgccaca
     1921 ccacagcacc aagctggcgt ggttgcgccg ctgagggatc atcgcctcgg cgtgccggac
     1981 ggccatgttc aggtagtcgc tgtcgcgcgg gggctcgttg tcgatgcccg aggacgactg
     2041 gcagaaggac tgccagacca tgataccggc ctcgtcgcac aggtcgtaga acagctcgcg
     2101 ctcaagcagc cccccacccc acacgcggag cagcgtgacg tgcgcctcct tcacgagctc
     2161 gagccagcgg tggtacttct cggggctctg gcggcggtag aggtgatgga tgggggccca
     2221 gttccagccc ttgatgaaga tgcggcgccc gttcacctcg atgacgtagg gcaacgcacc
     2281 cgctggggcg ctgtcgttgg tgatcatccg cacccggcgg aagccgatgc gcgtcgaccg
     2341 ctcgtcggcg gcgccgggga tctggatcca cgcgcaaacc gtgtacagtg gctggtcgcc
     2401 gtgcccgttg ggccaccaga gctgcgggtg gtcgacggac agggttgccg tgaccgtatg
     2461 ctcaccgggc tgggcctcga agggtaaggt ggcttccgtc acgatgcctt caccgccccg
     2521 gatgaccaca cgggccgccc ccgaagccgc gcggctggcc gagtaggcca cctcgaccgt
     2581 gacctgggcc gagcccaggt cgtcggacat tactggtcgc gcccacaggc cggtgatgcg
     2641 caggccggtg tcgcgcacga gttccacact cccccagagg cccagtggga ccaggcgggt
     2701 gcaccagtcc catccgtagg cgaagcgcgg cttccatgtg cggactcgcg aggtccagcc
     2761 gatctgccac tgctcatcgg gggcccgatc gaccacgacc gtcagcctgt tcggcgcgtc
     2821 ggcgcggagc tgttcggtga cgtcgacctc gaaaggaatg aaggcgccct cgtgctcggc
     2881 aagcttctgc ccgttcaggt gcacgtgagc gtggtagtcg acaccgtgga agacgagggt
     2941 ccaccgggcc ggcgagccct gccggagctc ctcgggcacc tcgagcacgc gcgcgtagat
     3001 ccagtcgcgg tcccaggtcc actcgcaggc gcggctctgg agctcgtagt agggatcggg
     3061 gatcacgccc tggtcgagga ggtcatcctg cacgctgccg ggcaccgtgg ccggacgcca
     3121 gcccggcccg cccggctcca ggtcctgggc cttggcccag atccactcgt tgggtagtcg
     3181 gctctgaatc caccaaccag cgccgtccag tgagatgcga tgcactgtcg tgttggggtc
     3241 gggcatggga gactccgttc gtcgatggga agctcgggcg cctgcggtgg cgacacgcgt
     3301 cagcgttcgc ccccgagccc gcgaggtcct ggctgtgcga gccgtcgtat ctcgggccac
     3361 gcaacggcaa gggcgttgtg ggcgtgcgcc tcgcgcagcg cgccgctccg ctcggcctcg
     3421 cggtagagcc agtaggccag ggcgatgacg ccctcctcgg ccgggcagcc ggccgcagtg
     3481 tagccttcgg ccgccgcgac tgggccgccc gcgacgacct gctggcccag cgacagcgtg
     3541 aggacgcgca ggtccgcatc accggcgatg ccctccaccg tcgatcgcag gtaggcatca
     3601 accgcttcgg cgatggcggc gagcacgtcg ccgattgcca cgggctggcc gtcctcgcgt
     3661 gtcacgcggc tgtgggcggt gaagaccgcc atgccggggc cgatggccgc ctgggcgaga
     3721 tcgaccggcg gagtgcctgc ccgcaccagg gcctccagcg ccaggggcac ctcccgcatc
     3781 agtgcctcgt ggcactggtc ccatgtcgtg gccggtgcgc cgtcctgtct tggccggcaa
     3841 gccagtatga cagccgaggc cagcgcgttg gtcccgtagg cgaggctgcg cgactggcgc
     3901 tcacttcgca gcggccacgc ggcggtaatg cgcagcccgg cgcccacgat ggccccgagc
     3961 atggcctccc agcctgcgga tgcgccactg gcgccgcctg atgccgcggc gggcttgagg
     4021 ccgtagtaga gaacgagcgg gaaccggtcg tcgtgcgcct cgcgaagccg cgctagggcc
     4081 tcagccatcc cgcgctcaaa gtggctgtgg gcatcgggga ggtcgcggaa gggcgaggcg
     4141 acgagctcat tggccttgct ggcctggtcc gtgccgcaga gacggatgag ggtctcgccc
     4201 aacgatcgct gcagccacac gaggaagaac tcggagagat cggcatactc gatgttgtcg
     4261 tagtagggcg ggtcggtgca gatgaccgcc cgtgttccag gcggccgcgg tgccgtggca
     4321 tcggcgacat aggcttggcc agcgggagtg cgggccggga gtccggccac accgtccgcc
     4381 acccactcga tgtggtgggc gaagctgccg accgagtatg agagtgggtt ggtctcgacg
     4441 tagtcccaga ccatgggcag cgcat
//
LOCUS       DAOSPL010000144         4463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_19578_length_4463_cov_3.752722, whole genome shotgun sequence.
ACCESSION   DAOSPL010000144 DAOSPL010000000
VERSION     DAOSPL010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19578_length_4463_cov_3.752722"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..144
                     /locus_tag="PLQ54_03900"
                     /pseudo
     CDS             1..144
                     /locus_tag="PLQ54_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
     gene            complement(547..1992)
                     /locus_tag="PLQ54_03905"
                     /pseudo
     CDS             complement(547..1992)
                     /locus_tag="PLQ54_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
     gene            2166..2639
                     /locus_tag="PLQ54_03910"
     CDS             2166..2639
                     /locus_tag="PLQ54_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92431.1"
                     /translation="MTAKRIVIALAVACAAVGAYLILTRQQMPEEEKIRRGIEDAAAA
                     FERGRVGSFMRIIADDYEDAYKVTRPMLVDMVREATLGGIANNTVTIEAMDIRLAGDT
                     MANVELTVTWVRKNPQGVPIGEATGRITAVMRKGDKGWQVVSSDGYESLASAYGV"
     gene            complement(2696..4060)
                     /locus_tag="PLQ54_03915"
     CDS             complement(2696..4060)
                     /locus_tag="PLQ54_03915"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOS92432.1"
                     /translation="MDAKARSEPAVRRWLARAASLHLRHPGSAILARAPGRLDVMGGI
                     ADYSGSTVLEGTLERAAWAAIEPRDDDQLVAVSVQAAAEDWADTVSWARGDLLSAPYD
                     QLRDAFAQDRRTHWAAYVLGAWAVLAREGVLTRGGSGATLALVSDVPIGAGVSSSAAI
                     EVASMAAIDRMLGLGLTGIELARLCQIVENRIAGAPCGIMDQVTSALGERGALLALRC
                     QPHDVLGNRVLPDGWSVYGISSAVRHVVGGGRYTRTRVAAFMGLEVIAHEAGVRPEGN
                     YLCRFTPAQYSAQFRHLLPSKLLGEEFSARYGGTADPVTKVRPGVRYSVRGCTEHPIY
                     ENHRVEQFMDLMADGSRRAVEKAGALMYGSHWSYGHRAGLGCAETDLIVRLVRERGPE
                     RGLMGAKITGGGSGGTVAVLTYGDARQAVNEVAAEYARLTGHRPQIFAGSSPGAIAWL
                     AGRR"
BASE COUNT          821 a         1506 c         1492 g          644 t
ORIGIN      
        1 cgcgccatgc agcagatcgc ggacaccggg cgcggcgtga tcgtctatat gcggcaggag
       61 gggcgcggca tcgggctggc gccgaagatc aaggcttaca agttgcagga acagggttac
      121 gacaccgtcg aggcgaacca ccagccgcgc cccaccatag cccgatgtgc cccggacgtg
      181 aacgatgaac cgcccatcgc gcggatagtc gacccggaag gtaaccgggt tgtgaagatc
      241 cgggtggttg accgtgccgt ggatgatccc gggaaccgtc tcggcgtcgg tcacggtgcc
      301 gtcgcggccc accgcgtgcg tgcggggctc gttggcaggg cccggtcccc aactgggtga
      361 ggccccggtc agcgtcaggt tggtgggctc ttcagccgga gcaccggcct ggtagcgcat
      421 cgctccgaac tccacgggca ccagccgctc gttggcgaag tccacgtcgg ccaggaagcg
      481 gctcagcgcc gcatagtggt agtacagatc ccgtggttcg acgtagttgt cccaccacca
      541 gagctgggcg cagccggccg actgcgccag tgacgtcgac cagatgccgt catgcagatg
      601 gatcccgtca gagtcggccc cattgccctc ggccatggcc ccggtgccga actcgcccac
      661 gatgtgcggc ttgccgaacc gctcgatctt ccgccgcgtg tagccgagca gaccctcagc
      721 cgtgtccagc gaaccgtagt tgtgagtctg gacatagtcc atctccggca gcccgtcgat
      781 ctccgggtcg ccgttcgagt tggcgaaact cgtggtgatc aaatgatcat acgggtcgat
      841 gtcccgaaga taccgcgcca tctcctggtg ccaggctctg acctcttcag ggacataccg
      901 ctcgatgatg tccacttcgt tccagaattc ccacgagagc acatgcgtgc tgtacgcgta
      961 ccgcgcgacg aggtgacgga gccgccaccg gaacaacttg cgggctggat cgctgacgaa
     1021 gaactcctcg ggtgtcgcgc agggaccccc attggccagg ttgtatggac aactagacca
     1081 atatgggtaa gggtcactgg ctcggagcga gttgaacgag tcgatgcaga gcatcaagta
     1141 gatgcccttc tcctccgcca ggctgagcac atagtccaga gcccaggcgc tggtcaggtc
     1201 gtgccggccg gccccgagaa cgccatcacc ctccggagct cgttcgagag cgaacagatc
     1261 aaacggaccc agccacagcc gagcgtagtt gcagccggcc tcggccatcc tcgtgaacca
     1321 ccggtcgtag tcgaatgtgc ctcgcgagcc cggccaacag acgttcatac ccagagcgaa
     1381 gtaaccctta ccggagtcaa aggcgaagta gtgcggatcc ctggcccggc ggacgaagcc
     1441 ctccgagccg gacgggacgc actcgaatgt ccgctcgtcc gactccacgg tccccgagcg
     1501 gtcgcgcacg cgaatccggt agcggtacgt gccggtctcg ggcgggcaga agcggatccg
     1561 gaacgtgtcc ccctcgaggc gagagagccg ttccgtatca ccctcgagcc gacgctcgta
     1621 ggggcggtga aggtagccgg gcacggccca ggtgtgcccg ccgggacccg agaactgggc
     1681 cgagacatcg atatcttccg ggtcgaatgg gttatcgtag gttgccgaga ggtcaaggat
     1741 ggcctcgagc ttgccatacc gcgggacctc cgtgggggcc gtgacgcccc gcaactcgag
     1801 gccctcggcc cgaggcccca cgaccttgag cggcagctgc ggctcgttcg gtgcgatcac
     1861 cagagcgccc aactccttcg ccggaacagt caggccgggc cgcgctcccg cccccgaacc
     1921 ggcaacgaag ggcagagcca gcgccagggc ggatgcgagc acgcgcggcg cgcggcggcg
     1981 ccagttgacc atgggggtcg cctccactca cggcccggga ccgagggccg tgaggcgagg
     2041 cttcggctcc gccgagcgca aggcctgctg cccagcccga cccggctgcg gcgcctcgcc
     2101 gcgcatctag tgaaactgaa gcgcacggcg gaggcaccag cacggggacg gagaggaagt
     2161 gcaccatgac agccaagcgg atcgtcatcg ccctggccgt cgcatgcgcg gctgtgggcg
     2221 catatctcat actgacacgc cagcagatgc cggaggaaga gaagatccgg cgagggatcg
     2281 aagacgcggc cgccgccttc gaacggggtc gcgtcggcag cttcatgaga atcatcgccg
     2341 acgactatga ggatgcctac aaagtaacgc gacccatgct cgtcgacatg gtacgggaag
     2401 cgacactcgg cggcatcgcc aacaacaccg tcaccatcga agcgatggac atccggcttg
     2461 ccggagacac catggccaac gtcgagctca cggtgacgtg ggtccgcaag aacccgcagg
     2521 gcgtcccgat cggcgaggca accgggagga tcaccgcggt gatgcgcaaa ggcgacaaag
     2581 gctggcaggt cgtctcatca gacggctacg agtccctcgc ctcggcatat ggcgtgtagc
     2641 ggcagcaggc agatcgcaga ggatgagagt caggactcac gagtccgagc ggctctcatc
     2701 ggcggccggc aagccaggcg atggcgccgg gagatgaacc cgcgaagatc tgcggacggt
     2761 gaccggtcag gcgcgcgtac tcggcggcca cctcgttgac ggcctggcgc gcgtcgccat
     2821 aggtcaggac cgccaccgtg ccacccgagc ctccgcccgt gatcttggcg cccatgaggc
     2881 cccgctcggg tccgcgttcg cggaccaggc ggacgatgag atcggtctcg gcgcaaccga
     2941 ggccggcccg gtggccgtag ctccagtgcg agccatacat cagggcaccg gccttctcga
     3001 ccgcgcgtcg cgacccatcg gccatgagat ccatgaactg ctcgacccga tggttctcgt
     3061 agatggggtg ctcggtacaa ccgcggaccg agtagcgaac accgggccgg accttcgtga
     3121 cggggtcggc ggtacctcca tatcgcgcgc tgaactcttc gcccaataac tttgacggca
     3181 acagatggcg gaactgagcc gaatactgag caggcgtgaa gcgacacagg tagttgccct
     3241 ctgggcgtac cccggcttca tgggcgatga cctcgaggcc catgaaggcc gccacccgcg
     3301 tgcgggtgta gcggccgccg cccaccacgt ggcgtaccgc cgagctgatt ccatagaccg
     3361 accagccgtc gggcagaacg cggttaccga gcacgtcgtg gggctggcac cgcagtgcca
     3421 gcagcgcccc tcgttcgccc agggcggagg taacttggtc cataatccca cacggcgccc
     3481 cggcgatgcg gttctcgacg atctggcaca gacgggccag ctcgatcccc gtcagcccaa
     3541 gccccagcat gcggtcgatg gcggccatgg aggccacctc gatggccgcc gacgacgaca
     3601 ctccagcacc gatcggcacg tcgctgacga gcgccagcgt ggcccccgat ccgcctctgg
     3661 tcaggacgcc ctctcgagcc aacacggccc aggcgcccag cacataggcc gcccagtgcg
     3721 tcctccggtc ttgcgcgaac gcgtcccgca gttggtcata tggggccgag aggaggtcgc
     3781 cgcgcgccca ggaaaccgtg tcggcccaat cctcggccgc ggcctggacg ctcaccgcga
     3841 ccaactggtc atcgtcgcgg ggctcgatag cggcccaggc agcccgctcg agagtcccct
     3901 cgaggacggt ggatcccgag tagtcagcga tcccacccat cacgtcgaga cgtcccggtg
     3961 cacgggcgag tatggcggag ccggggtgcc tcaggtgcag agatgccgct cgggcgagcc
     4021 atcgacggac cgccggctca gaccgcgcct tcgcgtccat ttggcctcca tcttgccgct
     4081 gaatcgggca caactacagt accgcaccgt ccccggcgcg tctgcgggca tggctcgcag
     4141 gccaaacaca gcagatagcc ggccgacaac gggccagcgg gccagaaccc gttcgctgcc
     4201 caccggctat tcgcctggaa ccaaacccgg aatggtgtgg gctgggcctt agtagccgcg
     4261 ttccctgcag gcacagggag cacctcggtc acgaggggcc gggctggggg acggggcgaa
     4321 tggaccgcgt cggccctcca cggggcgaac gggggagctg cgcgatggca gggcagggcg
     4381 gcgagggcag ccagtcgcgg gtactacccg accgcgtcag gcaggtcctc agccgagtgc
     4441 aggacatgcc aaccgtctcg acg
//
LOCUS       DAOSPL010000145         4462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_19583_length_4462_cov_2.930792, whole genome shotgun sequence.
ACCESSION   DAOSPL010000145 DAOSPL010000000
VERSION     DAOSPL010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19583_length_4462_cov_2.930792"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1857
                     /locus_tag="PLQ54_03920"
     CDS             <1..1857
                     /locus_tag="PLQ54_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92433.1"
                     /translation="RQWYRLALRVDDGYVEALIDGQVALRARTAGLTLGPCGLYAVDA
                     EHAAFDDVRVVPWHFYADDPATEGDGAWQAVQGEWTRDGGKLIARNSRGGALAVCGDP
                     SWTDDEVRATVHCGKAGTVGLVFGYRDRTHTYVLRTSDGESWQAVRRADGAETPLFSG
                     RGPRDGGQGIPVRVRRAGGIIQAWAGDQLLGCAGDSTFPDGQAGVLADAADGAAFSAF
                     QTRACDPPPPLPDLTDQFTQEATMVGWASPAGLWSSPTGDPPVQWYQAQVMGDAQIAA
                     YLPNAGGQEGTLILRVHGSPEAPGTGWSAQFAVHGAQGLGVQITANGQTLATAEGRPW
                     STEGPGHELRLVHQGPMVAAFADDTCLVTVTDPTPASHTGVAIGALGLPLDLMDVEVR
                     SPSLVEYTFSEAPSDWYVGRGVWEVTDRWKCSPGWAWLCGYKDDAPTLWSKRVFEGDQ
                     VAEVYCAMRMDIAEDPHYDRPSDLNMTLCGDGVRRDSGYTLIFGGWNPGRDRWNEAKS
                     ALLRGREIVAERPDVKFNVAGGNDTWHRHWFHVRMEKRGAQVLVDIDGQRVFEFTDPK
                     PLPGGRVAFWTYKNGYMIARTRVAFARGGQIEPIPPKPADDSDDIPFSLYGK"
     gene            complement(1879..3498)
                     /locus_tag="PLQ54_03925"
     CDS             complement(1879..3498)
                     /locus_tag="PLQ54_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92434.1"
                     /translation="MGACWQLKCALRRISARLERQRRLARMMWWSALGVLSTGCLGGL
                     MALGVLDLPGWAAVLPLVVCMAAGVASSARIRVSDREAARAADAALGLREELGSAVDL
                     SIEGQKGALVDALISSAVSRAETVRPEEVVPRLRPPVRQFRLLGLLGAIAAIAFLTAF
                     LWPRPPAERAAREAMKGESRRIIEIAKQVRAQAPTALREDTERLARHLEQLGRDFGRA
                     RLSKRDALERLGELTEELRRQQDALASNEAARDLQAAADRLRDTSFASEQAQAIAEGL
                     EAGDVEAARQEVRKLAEQMKKGQLSQEELKELARDLRKMAEAMKDSRQLAEAAEQLEK
                     AASACGNGQCDQAAKAAAAAAEALSNATEGGEKLTKAEAIEQLARELQDSQDAISGCK
                     GGGGAAREKSLLSPEELERGRFDPDVPGGPGPGGDSTNEAQAAGPAGQEGSPDITAKG
                     DPREAQYEELYEPRATPHGSKNTRVRGQMRDGGSSAMVDVKAPPTRSEIFSPYFSVEQ
                     AAQAAREDALDDRSIPREYRDRVKSYFQSLN"
     gene            complement(3534..3686)
                     /locus_tag="PLQ54_03930"
     CDS             complement(3534..3686)
                     /locus_tag="PLQ54_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92435.1"
                     /translation="MIWGVISLVVLICIMCGALTLAKMSTPGDTEAAGRSGPASSEMP
                     ATPAVD"
     gene            complement(3871..>4462)
                     /locus_tag="PLQ54_03935"
     CDS             complement(3871..>4462)
                     /locus_tag="PLQ54_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92436.1"
                     /translation="TPLEAGRTYYYAVIPVIKKLPPTPFPDTTASSGGGGGTSQTDMD
                     VQGTLVVGNTQYAGPATALIRPELVSPANYPEPGSQDVDPTGLLFQWITVAGANAYVI
                     EVSTDRSFPAGHTVRSSEILYNNTAGGQQVAFEFEGADLATAFRGYTDTIFWRIGAKR
                     EADAHVPVDLSSNRSIGWVFSTANGFQLETNPPGPP"
BASE COUNT          611 a         1520 c         1558 g          773 t
ORIGIN      
        1 cggcagtggt accggttggc gctccgcgtc gatgacggct atgtcgaagc actgatcgac
       61 ggccaggtgg ctttgcgggc gcggacggcc ggtctcacgc tgggcccgtg cgggctgtat
      121 gccgtcgatg cggagcacgc cgccttcgac gacgtccgcg tcgtgccgtg gcacttctat
      181 gccgatgatc cggcgacgga gggcgacggc gcctggcagg cggttcaggg cgagtggacg
      241 cgggatggcg gcaaactgat cgcgcggaac tcgcgtggag gtgccctggc ggtctgcggc
      301 gatccgtcct ggaccgacga tgaggtccgc gccacggtgc actgcggcaa ggcgggcacg
      361 gttggtttgg tgttcggcta tcgcgaccgg acccacacct atgtgcttcg cacttctgac
      421 ggcgagtcct ggcaggcggt gcggcgggcg gatggcgccg agaccccgct tttcagtggc
      481 cgggggcccc gggacggagg ccagggcatc ccggtccggg tgcgccgtgc cgggggcatc
      541 atccaggcgt gggcgggcga ccagctgctg ggttgtgcgg gcgattcgac cttccccgat
      601 ggccaggcgg gcgtgctggc cgacgccgca gatggcgctg cgttctcggc cttccagacc
      661 cgggcgtgtg acccacctcc gccccttccg gacctcacgg atcagttcac acaggaagcc
      721 accatggtcg gttgggcgag ccccgccggt ctgtggtcgt cgcccaccgg cgacccaccc
      781 gttcagtggt accaggccca ggtgatgggc gatgcgcaga tcgccgccta cctgccgaac
      841 gcgggcggac aggagggcac cctcatccta cgcgttcacg ggagccccga ggcccccggg
      901 acgggatggt cggctcagtt cgcggtccat ggcgcccagg gcctcggtgt ccagatcacc
      961 gccaatgggc agaccctcgc caccgcggag ggccgtccgt ggtccaccga gggtcccggg
     1021 cacgaactcc gcctggtgca tcagggcccc atggtcgcag cgttcgcgga cgacacatgc
     1081 ctcgtgaccg tgaccgaccc cactccggcg agccacacgg gcgtggccat cggggcactg
     1141 ggattgccgc tcgacctgat ggatgtggag gtccgctcgc cgagtctggt cgagtacacc
     1201 ttctcggagg cccccagtga ctggtacgtg ggccggggtg tctgggaggt cactgaccgg
     1261 tggaagtgct cgcctggctg ggcatggctc tgtggctaca aggacgacgc gccgaccctc
     1321 tggagcaagc gcgtcttcga gggcgaccag gtcgccgagg tctactgtgc catgcgcatg
     1381 gacatcgccg aggaccccca ctatgaccgc cccagcgatc tgaacatgac attgtgcggc
     1441 gacggagtcc ggcgcgactc gggctacacg ctcatctttg gcgggtggaa ccctggccgc
     1501 gaccgctgga acgaggccaa gtcggctctg ctgcggggac gggagatcgt ggccgagcgc
     1561 ccggacgtga agttcaatgt ggccggcggc aatgatacgt ggcatcgcca ttggttccac
     1621 gtgcgcatgg agaagcgcgg cgcgcaggtc ctggtggaca ttgacgggca gcgcgtcttt
     1681 gagtttaccg atcccaagcc tctgcctggt ggccgcgttg ccttctggac ctacaagaac
     1741 ggctacatga tcgctcggac gcgcgtggcc ttcgcccgag gcgggcagat cgagcccatt
     1801 ccccccaagc cggccgacga ctccgacgac atcccgttca gcctctacgg gaagtagggc
     1861 ccgggctggt tttcgggatc agttcagcga ctggaagtag ctcttgacgc ggtcgcggta
     1921 ctcgcgggga atggatcggt cgtcgagagc atcttcgcgc gccgcttggg cggcttgctc
     1981 gacactgaag tagggagaga agatctccga ccgggttgga ggcgccttta catcaaccat
     2041 ggcgctcgaa ccgccgtcac gcatctggcc gcggacgcgg gtgttcttgg agccatgtgg
     2101 ggtggcccgg ggctcgtaga gctcttcgta ctgtgcctcg cgggggtcgc ccttggccgt
     2161 gatgtccggc gatccctcct gtcccgcggg tccggcagcc tgtgcctcat tggtgctgtc
     2221 gccgcccggc ccggggcctc caggcacatc ggggtcgaac ctgccccgct ccagctcttc
     2281 aggtgacagc aggctcttct ccctggcggc gccccctcca cccttgcacc ccgagatggc
     2341 gtcctggctg tcctgcagct cgcgagccag ctgctcgatg gcctcggctt tggtgagctt
     2401 ctcaccgccc tcggtcgcat tggatagggc ttcagccgcg gcggcagccg ccttcgcggc
     2461 ctggtcgcac tgcccgttcc cgcaggcgct ggcggccttc tcgagctgtt cggccgcttc
     2521 ggcgagttgc cgggagtctt tcatggcttc ggccatcttc cggaggtccc gcgcgagctc
     2581 cttgagctcc tcctgactga gttggccctt cttcatctgc tcagccagtt ttcggacttc
     2641 ctggcgcgcg gcttccacgt ccccggcttc caggccctcc gcgatcgcct gcgcctgctc
     2701 ggaggcgaag ctcgtgtcgc gcagccgatc cgcagcggcc tgcaggtcgc gtgcggcctc
     2761 gttgctggcc agggcgtcct gctggcggcg caactcctcg gtgagttcac ccagccgttc
     2821 gagagcgtct cgtttggaga gccgcgcccg gccgaagtcg cgccctaact gctcaaggtg
     2881 gcgtgccagc cgttcggtgt cctcgcggag cgcggttggt gcttgggccc ggacctgttt
     2941 ggcgatttcg ataatccgcc gactctcgcc cttcatggcc tcgcgggccg cccgctccgc
     3001 cgggggacgc ggccacagga aggccgtgag gaacgcaatg gcggcgatgg cgcccagaag
     3061 cccgagtagc cgaaactgcc ggaccggagg ccgaagccgg ggcacgacct cctcggggcg
     3121 caccgtctcg gcgcgactca ccgccgagga gatcagggcg tcgaccaggg cgcccttctg
     3181 cccctcgatg gacagatcca ccgcactgcc cagctcctcg cgcagtccga gagccgcatc
     3241 ggccgcgcgg gcggcctcgc ggtcgctcac tcggatccgg gcgctggaag cgacgcccgc
     3301 cgccatgcac acgacgaggg gcagcacggc ggcccatcca gggaggtcca gcacacccag
     3361 agccatcagg ccgcccaggc atccggttga taggacgccg agggcgctcc accacatcat
     3421 tcgggcgagc cggcgctggc gctcgagccg cgcggagatg cgccggagag cgcacttgag
     3481 ttgccagcaa gcgcccatgg ctctaccgcc cttctcgcgc cggcaggtgc ctgctagtcc
     3541 accgccggcg tggccggcat ctcgctcgat gcgggcccgg accgccccgc ggcttcggtg
     3601 tcgccggggg tgctcatctt ggccagtgtc agcgccccac acatgatgca gatgagtacc
     3661 acaagcgata tgacgcccca gatcactgcg cggttgctcg accgcgcgac tcgtggcggg
     3721 cctggcggtg ttcccattcg tgcgcgtggt gtgcctcgtc gtgcgctcac ttggtctcct
     3781 cggccggcat ggcgccaaag cgcgcgcacc gcggaaggcc atcccgcggt gcgcctgatc
     3841 gcatctctac tcccgctccg caggcccagc ctagggcggg ccgggggggt tggtctccaa
     3901 ctggaaccca ttggccgtgc tgaacaccca gccgatactt cggttcgagc tcaggtccac
     3961 gggcacatgg gcgtcggcct cgcgcttggc gccgatgcgc cagaagatgg tgtcggtgta
     4021 gcctcggaag gccgtcgcca ggtcggcgcc ctcgaactcg aaggccacct gctgaccgcc
     4081 ggccgtgttg ttgtagagga tttcgctgct ccggacggtg tgccccgcgg ggaacgaccg
     4141 gtcggtgctg acctcgatca cgtaggcatt cgcgccggcc accgtgatcc actggaacag
     4201 caggcccgtt gggtcgacat cctggctgcc gggctcgggg tagttggccg gtgagaccag
     4261 ctcggggcgg atcagagccg tggccggtcc cgcgtactgg gtgtttccga ccaccagtgt
     4321 gccctggacg tccatatcgg tctgactggt acccccgccc ccacccgaac tcgccgtcgt
     4381 gtcggggaag ggtgtcgggg gcagtttctt gatcactggg atgaccgcgt agtagtaggt
     4441 ccggccggcc tcgagtggtg tg
//
LOCUS       DAOSPL010000146         4461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_19589_length_4461_cov_2.946664, whole genome shotgun sequence.
ACCESSION   DAOSPL010000146 DAOSPL010000000
VERSION     DAOSPL010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19589_length_4461_cov_2.946664"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PLQ54_03940"
     CDS             <1..241
                     /locus_tag="PLQ54_03940"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOS92437.1"
                     /translation="RAVASLPDLHREVVVLRFFHDLSLKEIAEVCHCSIGTVGSRLHY
                     AVKRLARELGPELGDAGGIGDEVLGDQTAPAGTRG"
     gene            195..1232
                     /locus_tag="PLQ54_03945"
     CDS             195..1232
                     /locus_tag="PLQ54_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92438.1"
                     /translation="MKCSETRRLLQEPEAKREAHPRYPELAGHLEACEACRHDQAEVE
                     TVSVLLREWPSIEPTVEDRQAAVAAMVAALAIRSRRPRSLAWSARFPAWRPASAATAL
                     AICVLLVLLWPSGPGADPVAAVCEALDGVSVWSAHGTITPAIIPIGGRPTNRIEVQFV
                     RPDTMRASMDGDLVQMLQQGDTRTWYVKPMRLAVIEKTEAVDFVKLFSVNEWLTNQAV
                     LATPARVVGEEWFLPQRVKTIEVRIADTHPDLARRVGLNTIILHANVDSMLPVHAQAE
                     GFAQLRDEIRALLTQAGVPLDPVRSQPSARNTASDYLTLHLHFGYGGAADPAGLKLDL
                     PEGTTVLDQRG"
     gene            complement(1248..4232)
                     /locus_tag="PLQ54_03950"
     CDS             complement(1248..4232)
                     /locus_tag="PLQ54_03950"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOS92439.1"
                     /translation="MNVPRKVWVRSAVVLVLAGGLYVFSRLYTQWLWCKSLGDSAGPA
                     HPYETVYIKTLLTSAYLLVASALGVFLIIYPNLRLSRRTPKRDDHYIGPSLFQYVDRE
                     MIDAHIDRWVLGSAALIAIFFGLTAKTRWFLLLQFIHAQPFGQTDPLFGLDASFYVFR
                     LPFLSFALGHLLSALVLAIAGTTLLYLYQDRIFITGGTVKVTPEVRRHLSVLIAAALV
                     LKAGAYWLARYKLLNVDRGLYGGPGYADVHGRIGVLTISAVLAVACALFFLATMRSGT
                     SVRLNGIALAIVFGFAIIAGNAYPRALQVYVKGGEKKLEMPYIERNIQATRAAYGLDR
                     IVATPFPAENRLTKQDLAEKETTVRSIRLWDHRPLRDVYAQKEQLYPYYQFVDIDVDR
                     YEINGRLRQVMLSAREINVDGLPPASQTWQNRHVVYTHGYGVSMSPVDRVTSEGLPEY
                     FLYGLPTESSGPIQLTQPRIYFGEIGHGPPGGSGSSSAGYDDRFAPRPPSRSTPQFVV
                     VGTKVEENDYPLGADKAQTTRYIGKDGVLLSGLFRRLIFALRLHSGYLVVSPDITSES
                     KIILNRDIRHRIHALCPWAVLAPDVDPYISIDDNGVPFWIIDAYTRTDRYPYSSVAGR
                     YGLNYIRNSVKITIDAYDGTTRFWVTDETDPLLKAYRSMFPALFSPLSEMPMDRQKHL
                     RYPLMMFHIQSMMLMRYHVENSGVFYSGEQQWVQPMELHGPMKTQAGQAPASQQPGPP
                     QAGMPRQVEGVQEAIAMSPYYVVMDVPGQKQDNLVLMLPFTRQGRPNLAAWMGASWSP
                     GTGARLDLFEFPENRTIWGPYQFEQKVDSDQVISQQLTLWTQGGSSVIRGNTLVIPIG
                     GSLLYVEPLYIQAHGTQGQGMSFPALKQVICAFGDRLAMRDTLAQALEAVLGIAPPPV
                     AVEPTAAGADGAPSVVPQATAPAPSPAPPSTTAEPVPPPASTPGVELPPELVQRIEER
                     LQEMEESLRETRSLLRQAAPKR"
BASE COUNT          827 a         1405 c         1477 g          752 t
ORIGIN      
        1 acgggccgta gcgtcgctgc cggatctgca ccgggaggtc gtggtgctgc ggttcttcca
       61 cgacctgtcg ctcaaggaga tcgcggaggt ctgtcactgc agcatcggca ccgtggggtc
      121 acgcctgcac tacgcggtga aacgcttggc ccgcgagttg gggccggagc tgggagatgc
      181 cggaggcatc ggggatgaag tgctcggaga ccagacggct cctgcaggaa ccagaggcta
      241 agagggaggc ccacccgagg tacccggaac tggccgggca cctggaggcc tgtgaggcct
      301 gtcggcatga ccaggctgag gtcgaaacgg tcagcgtact tctgcgcgag tggccctcga
      361 tcgagcccac cgtcgaggac cgccaggccg cggtggcggc catggttgcg gccctggcga
      421 tccgttcgcg gcggccgcgg tcgctggcgt ggtccgcgcg gtttcctgcg tggcgccccg
      481 cttcggccgc cacggccctc gccatctgtg tgctgctggt tctcttgtgg ccgtcagggc
      541 cgggagccga ccccgtggcc gcagtgtgcg aggccctgga cggcgtgagt gtctggtcgg
      601 cccacggaac gatcacgccc gcgatcatcc cgatcggggg gcggcccaca aaccgaatcg
      661 aggtgcagtt cgtccggcca gacaccatgc gggccagcat ggatggcgac ctcgtgcaga
      721 tgctccagca gggcgacacc cgaacatggt acgtcaagcc catgagactg gcggtgatcg
      781 agaagaccga agccgtggac ttcgtgaagc tcttctccgt caacgaatgg ctaaccaatc
      841 aggccgtctt ggccacaccc gcccgtgtcg tgggtgagga atggttccta ccccagcgag
      901 tgaaaacgat cgaagtgcgc atcgcagaca cacaccccga tctcgcccgt cgcgtggggc
      961 tgaacaccat catcctccac gccaacgtgg actccatgtt gccggtgcac gctcaggccg
     1021 agggtttcgc gcagctccgc gatgagatcc gtgccctcct gacccaggca ggcgtgccgc
     1081 tggaccctgt gcgctcccag ccctccgcac gcaacacggc atcagactat ctgaccctcc
     1141 acctccactt cggctacgga ggggcggcgg atccagccgg tctgaaactg gacctaccgg
     1201 agggcaccac ggtcctcgac cagcgcggct aggaccgcca tgtgcttcta ccgtttgggc
     1261 gcggcctgcc ggagcaggct acgagtctcc cgcaggctct cctccatctc ctggaggcgc
     1321 tcctcgatcc gctgcacgag ctcgggcggg agctccacac caggggtgga ggccgggggc
     1381 ggaaccggtt ccgccgtggt gctcggcggt gccggagacg gtgcgggagc cgtggcctgg
     1441 ggcaccaccg atggcgcacc gtcggcgcca gccgccgtcg gctccactgc gacgggtgga
     1501 ggcgcgatcc ccagcacggc ctccagggcc tgcgccagcg tgtcgcgcat ggccagcctg
     1561 tcgccgaacg cacagatcac ctgcttgagt gccgggaacg acatgccctg tccctgagtg
     1621 ccgtgggctt ggatgtagag cggctccaca tagagcagcg acccgccgat ggggatgacc
     1681 agtgtgttgc cgcgaatgac gctggagccc ccctgagtcc acagtgtcag ctgctgagaa
     1741 atcacttggt cgctgtcgac cttctgctcg aactggtaag gcccccagat ggtgcggttc
     1801 tccgggaact cgaacaagtc cagccgggcc ccggtacccg ggctccacga ggcgcccatc
     1861 cacgccgcca gattcggacg cccctgccgc gtgaaaggca gcatgagaac caggttgtcc
     1921 tgcttctgtc ccggcacgtc catcaccacg tagtaaggcg acatggcgat ggcctcctgc
     1981 acaccctcga cctgccgagg catgccggcc tgcggaggcc cgggctgctg ggacgctggc
     2041 gcttggcctg cttgcgtctt catgggcccg tgcagctcca tgggctgcac ccactgctgc
     2101 tcgccgctgt agaacacccc gctgttttcc acgtggtagc gcatcagcat catcgactgg
     2161 atgtgaaaca tcatgagcgg gtaccgaaga tgcttctggc gatccatggg catctcggag
     2221 agcggtgaga acaatgcggg gaacatgctg cggtaagctt taaggagcgg atcggtctcg
     2281 tccgtcaccc agaatcgcgt ggttccgtcg tacgcgtcga tggtgatctt caccgagttc
     2341 cggatgtagt tcagtccata gcggcccgcc acggaggagt aggggtagcg gtccgtccgc
     2401 gtgtaggcgt cgatgatcca gaagggtacc ccgttatcgt cgatggagat gtacggatcg
     2461 acatcgggcg ccagcactgc ccacgggcac agggcgtgaa tgcggtggcg aatgtcgcgg
     2521 ttgagtatga tcttgctctc tgaagttata tcaggactca ccacaaggta gccactgtgg
     2581 aggcgcaggg cgaaaatcag gcggcggaac aggccggaga ggaggacgcc atccttaccg
     2641 atgtagcgag tcgtctgcgc cttgtcggcg cccagagggt agtcattctc ctccaccttg
     2701 gtgcccacca caacgaactg aggcgtcgaa cgcgacggag gccggggcgc gaagcgatcg
     2761 tcatagccgg cgctggagct accggagccg cccgggggac catggccgat ctccccgaag
     2821 tagattcgcg gctgggtgag ctgaatgggt cccgacgact ccgtgggtag cccatacagg
     2881 aagtactcgg gcagcccctc agaggtcacg cgatcgacgg gactcatcga aacgccgtat
     2941 ccatgagtgt aaaccacgtg gcggttctgc caggtctggc tcgccggggg gagaccgtcc
     3001 acattgatct cgcgcgcact gagcatgacc tggcggagcc ggccgttgat ctcgtaacgg
     3061 tccacgtcga tatccacgaa ttggtagtag ggatagagct gctctttttg cgcgtagaca
     3121 tcgcggagcg ggcggtgatc ccatagccgg atcgatcgga ccgtcgtctc tttctcggca
     3181 agatcctgct tggtgagccg attctccgct gggaagggag tggcgacgat gcggtcgagc
     3241 ccgtacgccg ctcgagtcgc ctggatgttg cgttcgatat acggcatctc gagcttcttc
     3301 tcgccgccct tcacatagac ttgcagggct cgcggatacg cgttgcccgc gatgatggcg
     3361 aagccgaaca cgatagccag ggcgatgccg ttgaggcgga cactggtacc cgagcgcatt
     3421 gtggcgagga agaacagcgc acacgccacc gccagcaccg ctgagattgt gagcactccg
     3481 atccggccat ggacgtcggc gtagcccggt ccgccataga gtccgcggtc gacattcaac
     3541 aacttatacc tggccaacca gtaggcgccc gccttgagca ccaaggcagc ggcgatcagg
     3601 acagacaggt ggcgccggac ctcgggggtc accttcacgg tgccgccggt gataaagatc
     3661 cggtcctgat acaggtacag gagagtggtt cccgctatgg cgaggaccag cgcgctcagc
     3721 agatgcccca gggcgaagct caggaacggc agccggaaca cgtagaagga cgcatcgagc
     3781 ccgaacagcg ggtcggtctg gccgaagggt tgcgcatgga tgaactggag gagcagaaac
     3841 caccgggtct ttgccgtgag tccgaagaag atcgcaatca gtgctgccga gcccaagacc
     3901 caacggtcta tgtgagcatc gatcatctcg cggtccacgt actggaaaag gctcgggcca
     3961 atgtagtggt cgtcgcgctt gggggtccgg cgagacagac gcaggttagg ataaatgatc
     4021 agaaacacgc ccagtgccga cgccaccagc agataggccg aggtaagcag cgtcttgatg
     4081 tagacggtct cgtaagggtg cgccggaccc gcggagtccc ccaatgactt gcaccagagc
     4141 cactgggtgt agaggcggga gaacacgtag agcccgccgg ccagaaccag gaccacagcc
     4201 gaccggaccc agaccttcct gggaacattc atcgagtggg cttccaacct tccacgtcgc
     4261 gggatcaggc gcgccggcca caccatcggc ccgagcctga ggggcagcac gcactagtcg
     4321 tcgtgcaagt ccaggtcgag cagcaggtca cggaactgcc ggagctctcg ttgccgctca
     4381 tcggagtctt ctggaccgcg aatgccgagc ccttcggctg gcaaggacgc ctcttccatg
     4441 accgccgacg agacatagac t
//
LOCUS       DAOSPL010000147         4456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_19614_length_4456_cov_3.952056, whole genome shotgun sequence.
ACCESSION   DAOSPL010000147 DAOSPL010000000
VERSION     DAOSPL010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19614_length_4456_cov_3.952056"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PLQ54_03955"
     CDS             <1..537
                     /locus_tag="PLQ54_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92440.1"
                     /translation="IVGTLGIGFLAIEVALIQPLIRFLGYPTLTLSVVVFGLLIGGAA
                     GSLVAQRWPDGELPCRAAVAAACIALIGVALRLGLGPLFAAALPLPIIARSLIGLAVV
                     LPLGFALGIPFPTGIRMARGEFDRFIPCLWGINGLTSAVGSIGAMLIAREFGFPNACL
                     AGALLYLALIGLAPRIRG"
     gene            646..2037
                     /locus_tag="PLQ54_03960"
     CDS             646..2037
                     /locus_tag="PLQ54_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOS92441.1"
                     /translation="MPTKSGTSWSGVASLVAFCAACVAQGSDRTVPGNLLHNPDFGEA
                     SVSRLPTGWEAWSPRPDLAPRIAVLRPAGKGQASAVRLASTGHKTFGGLLQTVNGVAG
                     NQWYELRYRYRAEGIECPHDAVTVCLAWLDADGQLIEKQFVFEEEPGEGDWVLVVGKR
                     QAPERARQARVELLLRGASGGSVSFGQASLAATTPPAPRVARLATVCFVPSGHTPDEN
                     RRLWAEQCEAAGQQGADLVCLSEGIAMVGTGLSSYDSRETVPGPTTRVLGEVARRHRM
                     YIVAGVYEWAEGALWNTAVLIGRDGELVGRYRKTHLPEAEYVWGITPGTEYPVFETDL
                     GKIGLEICYDTFFPECARSLAVKGAEIICLPIWGDGREENTCWDVVSRARAIDNAVYF
                     VASIYSPKRSLIIDPWGHVLADTAGEEGVRVVEVDLNKRSLTPWLSVGGDGEWRTLWQ
                     KERRPSTYQGLAR"
     gene            complement(2137..3096)
                     /locus_tag="PLQ54_03965"
     CDS             complement(2137..3096)
                     /locus_tag="PLQ54_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92442.1"
                     /translation="MKKLLVLTGIVVASLAVCGCGKGGDEKSADSPKQGMSDFQTMNA
                     LAKAGSPEETIAIAQKAGGAVGAAASSAAGGGAGAAAAAAPALGGMLAAGGGLGAAPA
                     GAAPGAGPDEVPTGPEAERLQALIPLLQPGSLPPKAFEPRKGDLTYPRFERVIRVING
                     RCIDLNDTAIVYLAGVAAPVEGQPGYETARRVLTDLAYNRLVWVTSEEDRHVHAIAVR
                     PYALADEPKFIDLNASMAANLKDLRRADDYYHLRARWNGPHLVTANADVAAELAPRYA
                     EVEAARAALAKAVASQAEAQAAAEGGKGGAKLPPVAAAPKAGG"
     gene            complement(3174..>4456)
                     /locus_tag="PLQ54_03970"
     CDS             complement(3174..>4456)
                     /locus_tag="PLQ54_03970"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HOS92443.1"
                     /translation="TTLSQFVDDVPALGMAYVPAASYAEMLEWSGGLWRNFLRRYPES
                     NQMHKLMQRVSAKVDAMPTDRPDHEAARTELYRGQCNCPYWHGVFGGIYLLHLRHANH
                     AALLRAERLADASLRGDQAWQEVTIADLDYDGTDEVLIDGRELAAVLAPAKGGHLTAL
                     SAKREAHSLLATLSRRPEAYHERLASGEGALATEARRVREYDWYLRQALVDHFLAPGT
                     TPEQFRSSQYGELGDFVDRPYRVERAGGSPAEVVLVRDGHVWEGSVLGPLGIEKRVRV
                     DDAERRLSACYVLYHDADEPRRLWFAVEWNLSMTGGAEFGRFYRVDGEQEAARGLASM
                     GHRADVRTVELVDEWLGLAVELETVQPAEAWWQPLETVSQGVERVDLNYQASTVVLSY
                     HVELPARGHWRCQMSLALRAVPRTVMHASSALAP"
BASE COUNT          669 a         1518 c         1549 g          720 t
ORIGIN      
        1 atcgtcggca ctctcggcat cgggttcctg gccatcgagg tcgcgctgat ccagccacta
       61 atccgcttcc tcggctaccc cacgctgacc ctctcggtgg tcgtcttcgg cctgctcatc
      121 gggggcgcgg cggggagtct cgtggcccag cgctggcccg atggcgaact gccctgtcgg
      181 gcggccgtgg ccgccgcgtg catcgccctg atcggcgtgg cgctgagact cggtctgggg
      241 ccgctgttcg ccgccgctct gcctctcccc atcatcgccc ggtcgctgat cggcctggcg
      301 gtggtgctgc cgctggggtt cgcgctgggg atcccgttcc ccacgggaat ccgaatggcg
      361 aggggcgagt tcgaccgctt catcccgtgc ctgtggggga tcaacggtct gacatcggcc
      421 gtggggtcga tcggcgccat gctcattgcg cgcgagttcg gtttccccaa cgcctgcctg
      481 gccggggcgt tactctacct ggcgctcatc ggcctggcgc cgaggatcag aggctgaggc
      541 ccggggctcg gccctcggtc gcggaggccg cgggtctcgg cttcaccatc gatgagccag
      601 cccagctctg ggtccgggat aggcgacctg aaggaggcga tcccgttgcc gaccaaatct
      661 ggcacatcgt ggtctggggt tgcgtcgctg gtggccttct gcgctgcctg tgtcgcccaa
      721 gggagtgatc gcaccgtgcc gggtaatctg ctgcacaatc ccgactttgg cgaggcgtcg
      781 gtcagccggc tgcccaccgg ctgggaggca tggtcgcccc gcccggatct ggcgccgcgc
      841 atcgccgtcc tccggccggc tggcaagggc caggcctccg cagtccgcct ggcatcgacg
      901 ggccacaaga ccttcggcgg tctcctgcag accgtgaacg gagtggccgg caaccagtgg
      961 tacgagctcc gataccggta tcgggccgaa ggcatcgagt gtccacacga cgcggtgacc
     1021 gtgtgcctgg cgtggctcga cgccgacgga cagctcatcg agaagcagtt cgttttcgag
     1081 gaggagccgg gcgagggcga ttgggtgctg gtcgtcggca agcgacaggc gccggagcgg
     1141 gcgaggcaag ctcgggtcga gctgttgctc agaggggcgt caggtggctc cgtgtcgttt
     1201 ggccaggcct ctctggccgc cacgacgccg ccggctccgc gcgtggccag actggccacg
     1261 gtctgtttcg tcccgagtgg gcatacgccg gacgagaacc gtcggctgtg ggccgagcag
     1321 tgcgaggccg cggggcagca gggggctgac ctggtctgcc tgagcgaggg cattgccatg
     1381 gtgggcacgg gcttgtcgtc ctacgactcc cgtgagacgg ttcccggacc caccacacgg
     1441 gtgctgggcg aggtggctcg gcgccatcgc atgtacatcg tggccggcgt ctatgagtgg
     1501 gctgagggag ccttgtggaa cacggccgtc ctcatcggcc gggacggcga actcgtgggc
     1561 cggtaccgga agacccacct gcccgaggcc gagtacgtgt ggggcatcac tcccggcacc
     1621 gagtacccgg tgttcgagac cgacctgggc aagatcggcc tggagatctg ctatgacacg
     1681 ttcttccccg aatgcgcgcg gagcctggcc gtcaaagggg cggagatcat ctgtctgccc
     1741 atctggggcg acggccgcga ggagaatacg tgctgggacg tggtttcgcg ggcgcgggcc
     1801 atcgacaatg cggtctactt cgtcgcatca atctactccc ccaagcgcag cctcatcatc
     1861 gacccctggg ggcatgtgct ggccgacacg gcgggcgaag agggtgtccg tgtggtcgag
     1921 gtcgacctca acaagcggtc actgacccca tggctgagcg tcggcggtga cggcgagtgg
     1981 cgcacgctct ggcagaagga gcgacgccca agcacctatc aggggttggc ccgatagcgg
     2041 gagggggagt gccctggggg gagcgatgac ggcccggcag cggctcctcc atacaaggac
     2101 cggcggaaga ggggagtgac gctccccggc cacaactcac ccgccagcct tgggcgcggc
     2161 agcgacgggc ggcagtttgg cccctccctt cccgccctcg gcggccgcct gggcttcggc
     2221 ctgggaggca acggccttgg ccagcgccgc gcgagcggcc tcgacctcag catagcgagg
     2281 ggcgagctcg gcggccacgt cggcgttggc agtcaccagg tgcggcccat tccaccgggc
     2341 gcgcagatgg tagtaatcgt cggcccggcg aagatccttg agattggcag ccatgctggc
     2401 gttgaggtcg atgaacttgg gctcgtccgc cagcgcgtag ggccgcacgg ctatggcatg
     2461 cacgtggcgg tcttcctcgg acgtgaccca cacgagccgg ttataggcca gatcggtgag
     2521 cacacggcgc gccgtctcgt aaccgggctg cccctcgacg ggcgccgcga cgcctgccag
     2581 atagacgatg gccgtgtcgt tgaggtcaat gcagcgcccg ttgatgacgc ggatcacgcg
     2641 ctcgaagcgg gggtaggtga ggtcaccctt ccggggctcg aacgccttcg ggggaaggct
     2701 acccggctgc aggagcggga taagcgcctg gagccgctcg gcctcggggc ctgttggcac
     2761 ctcgtcgggg cctgccccag gggccgcccc ggccggggcc gcaccgaggc ctccgccggc
     2821 cgcaagcatg ccacctagag ccggcgcagc ggcagcggcg gcaccggcgc cgcccccagc
     2881 cgcgctgctc gcggcggctc cgacggcgcc accggccttc tgcgcgatgg cgatggtctc
     2941 ctccggcgac ccggccttcg ccagggcgtt catagtctgg aaatcagaca taccctgctt
     3001 gggtgaatcg gccgatttct cgtcgccgcc cttgccgcac ccacacacgg ccaggctcgc
     3061 gaccacgatc ccggtgagga ccagaagctt cttcacaaca gacgcctccc cgcttgcgcc
     3121 ttgcacgcgg aaactccgac cattctacat tcccccgccg cggccgcggc ccttcaagga
     3181 gccagtgccg aagaggcgtg catgaccgta cgcgggacgg cccggagcgc cagtgacatc
     3241 tgacagcgcc aatggccgcg ggcgggcagt tcgacgtgat agctcaaaac caccgtgctg
     3301 gcctggtagt tcaggtccac gcgttcgacg ccctggctta ccgtctcgag cggctgccac
     3361 caggcctcgg cgggctggac ggtctcgagt tccacggcta agcccaacca ttcgtcgacg
     3421 agctcaacgg tccgcacgtc ggcgcggtgc cccatgctgg cgaggcctcg agcggcctcc
     3481 tgttcgccgt cgacccggta gaagcggccg aactcggcac cgccggtcat ggacagattc
     3541 cactccacag cgaaccacag gcggcgaggc tcgtccgcat cgtggtagag cacgtaacac
     3601 gccgacagac gccgctccgc gtcgtcgacc cgcacgcgct tctcaatacc caaaggcccc
     3661 agcaccgatc cctcccacac gtgaccgtcg cgaaccagca cgacctcggc cggcgatccg
     3721 cccgcgcgtt ccacacggta gggtcggtcg acgaagtcac ccagctcacc gtactggctc
     3781 gagcggaact gctccggcgt ggtccccggg gcaaggaagt gatcgacgag cgcctgacgg
     3841 agataccagt cgtactccct cacgcgccgc gcttcagtgg ccaaggcccc ctcgcccgat
     3901 gccagtcgtt cgtggtaggc ctcgggacgc cgcgagagcg tcgccaacaa actgtgtgcc
     3961 tctcgcttgg ctgagagcgc cgtgaggtgg ccgcccttcg ccggggcgag aacggccgcg
     4021 agctcccggc catcgatcag cacctcgtcg gtgccatcgt agtcgaggtc ggcgatggtc
     4081 acctcctgcc aggcctggtc gccccggaga gaggcgtctg ccagtctctc ggcgcggagc
     4141 agcgccgcgt ggttcgcgtg ccgcagatgg agcaggtaga tgcccccgaa gacgccgtgc
     4201 cagtaggggc agttgcactg gcctcggtag agctcggtcc gcgcggcttc atggtcgggt
     4261 cgatcggtcg gcatggcgtc caccttggcg ctgacccgtt gcatcagttt gtgcatctga
     4321 ttcgactcgg ggtagcgccg caggaagttc cgccaaagac caccgctcca ctcgagcatc
     4381 tcggcatacg aggcggccgg aacataggcc attcccaggg ccgggacatc gtcgacgaac
     4441 tgactgagcg tcgtcg
//
LOCUS       DAOSPL010000148         4441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_19708_length_4441_cov_3.211810, whole genome shotgun sequence.
ACCESSION   DAOSPL010000148 DAOSPL010000000
VERSION     DAOSPL010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19708_length_4441_cov_3.211810"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1910
                     /locus_tag="PLQ54_03975"
     CDS             <1..1910
                     /locus_tag="PLQ54_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOS92444.1"
                     /translation="TTAFADRLLDDLALLDRWPERVRTMQRNWIGRSQGVEIDFVIEP
                     VGETITVFTTRVDTIFGATFVALAAEHPMAARLASGTPREAEVLAFIDAVRNEHATTR
                     GGEEADKVGVFTGGYAINPMNGERLPVWIANYILTGYGTGAIMAVPAHDQRDLEFARK
                     YDLPVRVVIQPEGDALDAETMTEAYVGEGTQVNSGQFDGLPSEQGKEAIADYMESQGF
                     GRRAVNYRLHDWCISRQRYWGAPIPIIYCEEHGPVAVPESDLPVLLPEDADLRESGRS
                     PLASSPAFVNAPCPKCGKPGRREVDTMDTFVDSSWYFLRFASPHEETVPFDRAAVDYW
                     LPVDQYVGGVEHAVLHLYYARFVTKALHDLGYLSFVEPFAELFTQGMIVKDGAKMSKS
                     RGNVVRPDAIIDKYGADALRLFILFAGPPDQDAEWSDEGVAGCFRFLSRAFSAVANHT
                     DAYDPEWRHALASAEFSPAAAAIRRKLHQTIAKVTSEIEERFHFNTAIAALMEYVNDL
                     TPFLEGGLDGIQERIVFSEAADTLTLLLSPFAPHLADELWERLGHEGSTYSQAWIDAD
                     PEVAREESIEIPVQVNGKVRGRVVVPVDAAEDEVKRLALELDRVRAQLEGKQVRKVIV
                     AAGRLVSIVAS"
     gene            2299..3369
                     /locus_tag="PLQ54_03980"
     CDS             2299..3369
                     /locus_tag="PLQ54_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS92445.1"
                     /translation="MCTMAKAKKAIKTSPKKSLPSEIRMAVIGYGGAFNMGKAHASWG
                     QAAPGFRFVAACDIDPARAAAAAEDFPGIETYTDVDTMLRKCDFDLVTIVTPHNTHAP
                     LAIQCARAGKHVVVEKPMCITTQEGTDMIAAAKKAGTCLTVFHNRRHDGDYLAVKEIV
                     DKGMIGDVFKVEMCMGGYDAPGTWWRSDKAISGGAFYDWGAHILDWLLHVVPQRIEHV
                     MGYFHKLVWHQMTNEDHVHAVIKFVNGATADVQLSTIQRIGKPRWRILGTKGAIVDTG
                     QGKFHVVTELKGMVVEMDVPYRQSSWQNFYDNLSAHLLKGKPLEVTPESARRVIAIME
                     AAEVSSQTGQPQPVPYDDTLHL"
     gene            3431..3991
                     /locus_tag="PLQ54_03985"
     CDS             3431..3991
                     /locus_tag="PLQ54_03985"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOS92446.1"
                     /translation="MRRGTMPYLVGELVDLAPPGDDLLPYFVTWENDPALLRLSDSPS
                     QAPRGWDGARSMFDSLRSGPGLFFGIYTKGGHPIGYLGANIDERDRRACVYIVVGDAG
                     YQGRGYGTDAMRVLLRHLFDGLGLHRAYLFVHESNQRAIRCYEKCGFVWEGRLREHLR
                     EGSTYSAEIAMGVLADEFRAVDPGPP"
     gene            4076..>4441
                     /locus_tag="PLQ54_03990"
     CDS             4076..>4441
                     /locus_tag="PLQ54_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HOS92447.1"
                     /translation="MLVSIGAVIVSLAVILAGAELFTNSIEWLGRRMRLAEGAVGSIL
                     AAVGTALPETLIPIIAIVFEQGEHADSIGIGAILGAPLMLSTLAMFVTGFAILICSMR
                     GHRPREVVADAGGQVDVPLA"
BASE COUNT          752 a         1533 c         1404 g          752 t
ORIGIN      
        1 ggaccacggc gttcgcggac cgcctactcg atgatctggc gctgctcgac cgctggcccg
       61 aacgggtccg caccatgcag cggaactgga ttggccggag ccagggtgtc gagatcgact
      121 tcgtcatcga gcccgtggga gagaccatca ccgtcttcac cacccgggtc gacacgattt
      181 tcggggcgac attcgtggcc ctggcagccg agcaccccat ggcggctcga ctcgccagcg
      241 ggacccctcg cgaggccgag gtgttggcct tcatcgacgc ggtacgcaac gagcatgcca
      301 cgacccgtgg cggcgaagag gccgacaaag tgggcgtgtt cacgggcggc tacgccatca
      361 accccatgaa cggcgagcga ctgccggtct ggattgccaa ctacatcctc acgggctatg
      421 gcaccggcgc catcatggcg gttcccgcac acgaccagcg cgacctcgag ttcgctcgca
      481 agtacgacct gccggtgcgc gtggtgatcc agcccgaggg cgacgccctg gatgctgaga
      541 ccatgaccga ggcctatgtg ggggagggga cgcaggtcaa ctcgggccag ttcgacggcc
      601 ttccctccga gcagggcaag gaggccattg ccgactacat ggaatcccag ggcttcgggc
      661 ggcgcgccgt caactaccgc ctccacgact ggtgcatcag ccggcaacgc tactggggcg
      721 cacccatccc catcatctac tgcgaggagc atggccccgt ggccgtgccc gagagcgacc
      781 tgcccgtgct gctgcccgag gacgccgacc tccgcgagtc cggccggtca cctctggcct
      841 cgtcgcccgc cttcgtcaac gcgccgtgcc cgaagtgtgg caagccgggc cggcgcgagg
      901 tcgacaccat ggacaccttc gtcgactcct cctggtactt tctccgcttc gccagccccc
      961 atgaggagac tgtgcccttc gaccgggccg ctgtcgacta ctggctgccc gtcgaccagt
     1021 atgtcggcgg tgtcgagcac gcggtgctgc atctctacta cgcccggttc gtcaccaaag
     1081 cgctgcacga cctgggatac ctgagcttcg tcgaaccttt cgccgagctc ttcacccagg
     1141 gcatgatcgt caaagacggc gccaagatgt ccaagtccag gggcaatgtc gtccgtcccg
     1201 acgccatcat cgacaagtac ggcgccgatg cgctgcgcct cttcatcctg ttcgccgggc
     1261 cgcccgacca ggacgccgaa tggagtgacg agggcgtcgc gggatgcttc cgtttcctct
     1321 cccgggcttt ctctgccgtg gcgaaccaca ccgatgccta cgaccccgaa tggcggcatg
     1381 ctctagcctc ggccgagttc tcccctgccg ctgcggcgat ccgccggaag cttcaccaga
     1441 ccatcgccaa agtcacctcg gagattgagg aacgcttcca tttcaacacg gccatcgccg
     1501 cgttgatgga gtacgtcaat gatctgaccc cattcctcga gggcggcctc gacggcatcc
     1561 aggagcgcat cgtgttcagt gaggccgccg acaccctgac tctgctgctc tcgccgttcg
     1621 cgccccacct ggccgacgag ctgtgggagc ggctgggcca cgagggcagc acctactcgc
     1681 aagcctggat cgacgccgat cccgaggtgg ctcgcgagga gtccatcgag atcccggttc
     1741 aggtgaatgg caaggtgcga ggccgcgtcg tagtccccgt cgatgccgcc gaggatgagg
     1801 tcaaacgcct ggccctcgag ctcgatcgtg tcagagccca gctcgaggga aagcaggtcc
     1861 gcaaggtcat tgtggccgcg ggccgactcg tcagcatcgt ggcctcttag gcccgcgatc
     1921 ccgctgccga gcccttcgcc acccgcgcac ggagtccgat ggagccgaga cgcaggttca
     1981 tcttctacct ccgacccggg gagcggcggg tgatgtgggg actcatcgcc ctcctcgccg
     2041 tcctggcatc gctgctgctc tggcagaagt ggcacgcctg gcacatgccg ccgctgataa
     2101 ccccgccggc tccacctctc ccttaagatc gagtcgcgca cggccgatcc ggggttctgt
     2161 cttcatggcc caacccctgg gcccgcggcc cgcggggtca gttgggggcc gacgggggtg
     2221 cccatgcacg acaaggagtc cccgcgcggg cgccgaaccg agcagtgtgc gaactgatcc
     2281 gagacacgaa gggtgtgcat gtgcaccatg gccaaggcca agaaagccat caagaccagc
     2341 ccgaagaagt ccctgccatc ggagatccgc atggccgtca tcggctatgg cggcgcgttc
     2401 aacatgggta aggcccatgc ctcctggggg caagcggcac cgggtttccg cttcgtcgcc
     2461 gcgtgcgaca tcgacccggc tcgcgccgcc gccgccgccg aagacttccc cgggatcgag
     2521 acctacaccg acgtcgacac catgctgcgc aagtgtgact tcgacctggt gaccatcgtc
     2581 acgccccaca acacgcacgc gccactggcc atccagtgcg ctcgagccgg caagcacgtc
     2641 gtcgtcgaga agcccatgtg catcaccacc caagagggca ccgacatgat tgccgccgcc
     2701 aagaaggccg gcacctgcct caccgtcttt cacaaccgcc ggcacgatgg cgactacctg
     2761 gcagtcaagg agatcgtgga caagggtatg atcggcgacg tgttcaaagt cgagatgtgc
     2821 atgggcggct acgacgcacc cggaacgtgg tggcggtccg acaaagccat ctccggaggt
     2881 gccttctatg actggggcgc ccacatcctc gactggctcc tccacgtggt gccgcagcgc
     2941 atcgagcacg tcatgggtta cttccacaaa ctggtgtggc accagatgac caacgaggac
     3001 cacgtccatg ccgtgatcaa gttcgtgaac ggtgccacgg cggatgtgca gctctcgacg
     3061 atccagcgca tcggcaagcc gcgttggcgc atcctcggca ccaagggagc catcgtcgac
     3121 acgggccagg gcaagttcca cgtcgtgacc gagctcaagg gcatggttgt cgagatggat
     3181 gtgccgtacc gccagagcag ctggcagaac ttctacgaca atctgtccgc gcatctgctc
     3241 aagggcaagc cgctcgaggt aacccccgag tccgctcggc gtgtcatcgc cattatggag
     3301 gccgccgagg tctcttcgca gaccgggcag ccccaaccgg tgccctacga cgacacactg
     3361 cacctctagc gtcagtgccc gccacctgcg gcgtcgtggt gcgcggagcc aatgaaaggt
     3421 aggcgcgagc ttgcggcgcg ggaccatgcc gtatctggtg ggcgagctgg tcgacctggc
     3481 tccgccgggt gacgatctgc tgccctactt tgtgacgtgg gagaacgacc cggccctcct
     3541 gcgcctatcc gacagcccct cgcaggcccc gcgcggatgg gacggggccc ggagcatgtt
     3601 tgactccctc cgctccgggc ctgggctctt ctttgggatc tatacaaaag gggggcaccc
     3661 catcggctat ctgggcgcga acattgacga gcgcgaccgg cgggcttgcg tgtatattgt
     3721 ggtcggagac gcggggtacc aggggcgcgg ctacggcacc gatgccatgc gcgtgctcct
     3781 gcgccacctg ttcgatggac tggggcttca ccgggcctac ttgttcgtgc acgagtccaa
     3841 ccagcgggcg atccgctgct acgagaagtg cggtttcgtc tgggagggcc gcctccgcga
     3901 acacctgcga gagggctcca cttactccgc ggagattgcc atgggagtgc tggcggacga
     3961 gttccgcgcc gtcgatccgg ggcccccata gcatcgcctc tgatctgcgc ctgtggtcgg
     4021 ctctccgtgg ttggccgggc gccttcacac cgacaccgaa agcagtggtc gcttcgtgct
     4081 cgtctcgatc ggggctgtca tcgtcagcct ggccgtgatc ctcgccggcg ccgagctatt
     4141 caccaacagc atcgagtggc tgggacgccg catgcgcctc gctgaagggg ccgtcggcag
     4201 catcctcgcc gccgtgggca ccgcgctccc cgagacactc atccccatca tcgccatcgt
     4261 gtttgagcag ggcgagcacg ccgacagcat cggcatcgga gccattctcg gcgccccgct
     4321 catgctcagc actctggcga tgttcgtgac ggggttcgcc atcctgatct gctccatgcg
     4381 gggtcatcgc ccccgagagg tcgtcgccga cgctggcggg caggtcgacg tcccactggc
     4441 g
//
LOCUS       DAOSPL010000149         4440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_19712_length_4440_cov_2.926340, whole genome shotgun sequence.
ACCESSION   DAOSPL010000149 DAOSPL010000000
VERSION     DAOSPL010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19712_length_4440_cov_2.926340"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            339..2822
                     /locus_tag="PLQ54_03995"
     CDS             339..2822
                     /locus_tag="PLQ54_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOS92448.1"
                     /translation="MPSALVAVCIALALAPLAATAASTYYVSPGGDDAWSGTLPAPNG
                     ARTDGPFATPAGARDAVRAARAAGRAAGDAVVFIRAGQYCLEETLALAAEDSGLTFAA
                     YRDEKPQIIGGRLISGLKPGADGVWSVELPDVKAGRWSFRSLFVDGKRMIRARHPNFD
                     PSDPYRKGFAYAAKDPSGFGVAVGCIHNVGDWMDYTINVPAAGEYTFWVYYGALNEPF
                     GNATMDGRTAIRIDGGEPVLLNDLPDTGGWAASRWGRCATLTLTAGEHTLRWENLKGG
                     GLDLEAYALCDDPAWVPAGTNLPPAAAGKHVLTIQAENFTAYHGPQLQVAGSGATAKD
                     RFFFAPGDIRPEWAQAPEAEVHIFQTGNCRAFKEICWIDRIDADARCIYLKGPECTSD
                     LYQGDRYFVENVRDALDAPGEWYLDREAGVLYLQPPAGFTRESEVMAPTIGRIVEAEG
                     VTGISFEGLTFRGGDWDFGDGCEAYGMGTNGIIYLKDSTRCGVTGCTFANIGKDAVCV
                     EGGHGHEVVRNDVSDSAEGGININGASDVTVSRNHIHHCGQVYKHNSGVTLQNGASAC
                     RVRANVIHDMTRYGITMKMAGHDNLIEHNRVLNTSLETYDTGAIEVTQQDKEDRSGTR
                     IIGNIVGDTIGYSSTEGTPCFLSWSIYLDSFAGGYTVERNVCFRAQYGGIMFQGGKDN
                     HVTNNIFVGGQVANGHISNFAGNQTGCTLERNLFCLSNPQAQLFAAGHLTPEVIRMGS
                     NLYWCPEGGEGWQATLKAWQESGLDQGSVIADPLFVDADADDYRLKPDSPALMLGFKP
                     IDLSRVPSCDCHIEKLGPVSFGNAPWPAK"
     gene            complement(2967..>4440)
                     /locus_tag="PLQ54_04000"
     CDS             complement(2967..>4440)
                     /locus_tag="PLQ54_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HOS92449.1"
                     /translation="AILERVRRFSGKLYLEFGGKLTFDFHAARVLPGYDPNVKMRLLE
                     GLRDQIEVVLCVCSEDLERGRIRGDFGITYDLATLKAMDDLRDWGITVSAVVLTQYRE
                     GPTSRRLMGQLEAKGIRAYTHTAIPDYPNDVDLIVSDNGYGRNAYVETEKPIVVVTGT
                     GPGSGKMSTCLSQLYHDHQRGTASGYAKFETFPIWSLPVEHPVNLAYEAATADLADIV
                     MIDPFHLEAYGVKAVNYNRDIENFPILKAIIDRISGGKTTATTYRSPTDMGVNRAGSG
                     IVDDAAVSAASVQEVIRRYFRHNWERQRGLCSADTVRRVQKLMVRLGVSATDRPVVEP
                     ARAAAAEAEKNQTGNDGFFCGAALELPGGAIVTGTNSPLFHSASAALISGIKVLAGLP
                     RYIHLLPASIIESVTALKAGYLGSNSPSLNVQEVLVALGISAASNPAAKAGVEMLPQL
                     RGAQMHLTHEPGAGDETGLRKLGVDVTTDANPTSRGYFLR"
BASE COUNT          743 a         1526 c         1460 g          711 t
ORIGIN      
        1 gagcatgggc cagatctgca cgaagtcgta cccgatcgag cgcaagcccg tgaggtagct
       61 gcgccagtct tcttcgctcc atgtgcgcgc cgcatagggg tggctatagc cccagtgcat
      121 gtggatgtac atcccgagca tgggcggtct ccttctaccc ggcacaccgc atgagccgag
      181 agagcaacag ttacgccatc gggtgtccgg ggaccttgcg ggcgggcagc ccatcccgtg
      241 ggcggtacta cgtccgctcc cgcctgtggc ggtggcgcgc tcagcccccg caggtgcccc
      301 cccaccccca cgggaacccc cacactgagg tgaccaccat gcccagcgca ctcgtggccg
      361 tgtgcatcgc gctcgccctc gcccccctcg ctgccaccgc ggcttccacc tactacgtct
      421 ctcccggcgg cgacgatgcc tggtctggca cgctgcccgc gccgaatggg gccaggaccg
      481 atggcccgtt cgccacaccc gcgggtgccc gggacgccgt ccgtgccgcc cgggccgcgg
      541 gccgagcggc tggggatgcg gtggtcttca tccgggcggg ccagtactgc ctcgaagaga
      601 cgctggcgct cgccgcggaa gacagcggcc tcaccttcgc cgcctaccgg gacgagaagc
      661 cacagatcat tggcgggcgg ctcatctccg gcctgaagcc cggcgcggat ggcgtctggt
      721 ccgtcgaact gcctgatgtc aaagccggcc ggtggagttt ccggtcgctg ttcgtcgacg
      781 gcaagcgcat gatccgcgcc cggcacccga acttcgaccc gagcgatccc taccgcaagg
      841 gcttcgccta cgccgcgaag gatcccagcg gcttcggcgt cgcagtgggc tgtatccaca
      901 acgtgggcga ctggatggac tacacaatca acgtaccggc ggccggcgag tacaccttct
      961 gggtctacta cggcgcgctc aacgagccct tcggcaatgc gacgatggac ggccgcaccg
     1021 ccatcaggat cgacggcggc gagccggttc tgctcaacga cctgcccgac actggcggtt
     1081 gggcggcctc ccgctggggc cggtgcgcca cgctcactct cacggccgga gaacacacgc
     1141 tccgctggga gaacctcaag ggcggcggtc tcgatctcga ggcctatgcg ctgtgcgacg
     1201 atccggcctg ggtgcccgcg ggcaccaacc tgccgccagc cgccgccggg aagcatgtgc
     1261 tgaccatcca ggccgagaac ttcacggcct accacggccc gcaactgcag gtcgccgggt
     1321 cgggggccac ggccaaggac cggttcttct ttgcccccgg cgacatcaga cccgagtggg
     1381 cacaggcccc cgaggccgag gtgcacatct tccagaccgg caactgccgg gcgttcaagg
     1441 agatctgctg gatcgaccgg atcgatgccg atgcccgttg catctacctg aaggggcccg
     1501 agtgcacctc ggacctgtat cagggtgacc gctactttgt cgagaacgtg cgcgatgcgc
     1561 tcgacgcgcc cggcgagtgg tacctcgacc gcgaggccgg cgtgctctac ctccagccgc
     1621 cagccgggtt cacgcgggag tcggaggtca tggctcccac gatcggccgc atcgtcgagg
     1681 ccgagggcgt cacgggcatc tcgttcgagg ggctcacctt ccgcgggggc gactgggact
     1741 tcggcgacgg ctgtgaggcc tacggcatgg gcaccaatgg catcatctac ctgaaggact
     1801 ccacgcggtg cggggtcacc ggctgcacct tcgccaacat cggcaaggat gccgtctgtg
     1861 tcgagggcgg acacgggcac gaggtcgtga ggaacgacgt cagcgacagc gccgagggcg
     1921 gcatcaacat caacggtgcc agcgatgtca ccgtgtcccg caaccacatc caccactgcg
     1981 ggcaggtcta caagcacaac agcggcgtca cgctgcagaa cggcgccagc gcctgccgcg
     2041 tgcgcgccaa cgtcatccac gatatgaccc gctacggcat caccatgaag atggccgggc
     2101 atgacaacct catcgagcac aaccgcgtgc tcaacaccag cctcgagacc tacgacaccg
     2161 gggccatcga ggtgacgcaa caggacaagg aagaccgcag cggcacgcgg atcatcggca
     2221 acatcgtggg tgacaccatc ggctactcca gcaccgaagg gacgccttgt ttcctctcgt
     2281 ggagcatcta cctcgacagt ttcgccggcg gctacacggt ggagaggaat gtgtgcttcc
     2341 gcgcccagta tggcgggatc atgttccagg gcgggaagga caaccacgtc accaacaaca
     2401 tcttcgtcgg cggacaggtc gccaatgggc acatctccaa cttcgccggc aaccagacgg
     2461 gctgcacact cgagcggaac ctcttctgcc ttagcaaccc ccaggcccag ctcttcgccg
     2521 cggggcacct cacgcccgag gtgatccgca tgggctcgaa cctctattgg tgtcccgagg
     2581 gtggcgaggg ctggcaggcg acgctgaagg catggcagga gagtgggctc gaccagggct
     2641 cggtcatcgc cgacccgctg ttcgtcgacg cggacgccga cgactatcgg ctgaagcctg
     2701 actcgccggc tctcatgctc gggttcaagc ccatcgacct ctcgcgggtg ccgtcgtgcg
     2761 attgccacat cgagaagctg ggccctgtgt ccttcggcaa cgcgccctgg ccggcgaagt
     2821 gaggcgacgg tccgtagaca gacggcaccg tctcgggctc gagctggccg cgtctggcgc
     2881 tcgggcggca gccggatggg agccgcgagg ggctggcccc tcgcgcgggg tgcccggcag
     2941 agccccgcga tctccccgat gggcccttac cgcaggaagt agcctcgcga agtcgggttc
     3001 gcgtcggtcg tcacgtccac gcccagcttg cggaggccgg tctcgtcgcc cgccccgggc
     3061 tcatgggtga ggtgcatctg agcgcctcgg agctgcggga gcatctccac gccggccttc
     3121 gcggcagggt tgctggccgc actgatgcca agcgcgacca gcacctcctg cacgttgaga
     3181 ctgggggagt tcgagcccag atagcctgcc ttcagcgccg tgacactctc gatgatgctg
     3241 gcaggcagca ggtggatgta ccggggcagg cccgcgagaa ccttgatgcc gctaatgagt
     3301 gccgccgagg cggagtggaa gagaggggaa ttggtgcccg tgacgatcgc gccgcccggc
     3361 aactcgagcg cggccccgca gaagaagcca tcgttgcccg tctggttctt ctcggcctcg
     3421 gcggccgccg cgcgggccgg ctcgaccacc ggtcggtctg ttgccgacac gcccaggcgg
     3481 accatcagct tctggacacg acggaccgtg tccgccgagc acagcccgcg ctggcgctcc
     3541 cagttgtgcc ggaagtaccg gcggatcacc tcctggaccg aggctgccga tacggccgcg
     3601 tcatccacga tgcccgaccc cgcgcggttc acacccatgt ccgtcggcga ccggtaggtt
     3661 gtggcggttg tcttgccgcc cgaaatgcgg tcaatgatgg ctttgaggat ggggaagttc
     3721 tcgatgtccc ggttgtagtt gaccgccttg acgccgtacg cctccaggtg gaacggatcg
     3781 atcatgacga tgtcggccag atcagcggtc gcggcctcgt aggccaggtt caccggatgc
     3841 tcgaccggca ggctccagat ggggaaagtc tcgaacttcg cataccccga cgcggtgccg
     3901 cgctggtgat catggtagag ctgcgaaagg caggtcgaca tcttcccgct gcccgggccc
     3961 gtgccggtca cgacgacgat gggcttctca gtctccacgt acgcattacg gccatacccg
     4021 ttgtcagaca cgatcaggtc cacgtcgttg gggtagtcgg ggatggcggt gtgggtgtag
     4081 gcccggatcc ctttggcctc gagctgcccc atcagccgac gggacgtcgg tccctcgcgg
     4141 tactgggtga gcaccacggc cgagacggtg atgccccagt cgcgtagatc gtccatcgcc
     4201 ttcagcgttg ccaggtcgta ggtgatgccg aagtcgccgc ggatccgccc tcgctccagg
     4261 tcctccgagc acacacacag caccacctcg atctggtccc tcagcccttc gagcaagcgc
     4321 atcttcacgt tagggtcgta tccgggcagc acgcgtgcgg cgtggaagtc gaacgtcagc
     4381 tttcccccga actccaggta cagcttgccg gagaagcggc ggacacgctc gaggatcgcc
//
LOCUS       DAOSPL010000150         4435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_19733_length_4435_cov_3.072146, whole genome shotgun sequence.
ACCESSION   DAOSPL010000150 DAOSPL010000000
VERSION     DAOSPL010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19733_length_4435_cov_3.072146"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="PLQ54_04005"
     CDS             <1..1042
                     /locus_tag="PLQ54_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92450.1"
                     /translation="DESNFLGFLYPWDLCRETSDDNSLKAAIHVSELDLISCPCAPIT
                     FNAVWVTHGRAHGMAFQEGGGFVRGGGTSSDDATAGTVAFFRNANGASGWETIGSFEP
                     DATGRWRTPPAKELGYTYGVKHRSMASHDDMGEFRTREYQYGEVTVTEELLLGCCSIA
                     RDAMGCPIWAACELEGTVNAYALNDFDFWHCPGNPALTGAAYKYASICACDDGSIIVG
                     ATNTDQGRIDVVRSRNNGTTWEAVNTAFGTGLVNGHLFHIHGQVYLVGWASDKVWLVT
                     SSQADLTREVMDGTNTILEVCAHAVGAGEDAPQCSVIIDDTGQIIVAVAGDADTRFYS
                     CRNFRTGFSLVA"
     gene            1118..3334
                     /locus_tag="PLQ54_04010"
     CDS             1118..3334
                     /locus_tag="PLQ54_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92451.1"
                     /translation="MSLIRTEGSLVVYHPAADGIPTVQGFSRDWYPSAKWESAGGCEF
                     IKPINAIAPAGDLRTLAMTATFAIDASYNQSLRQIITPGNPPVASYEFYDNSAYASGT
                     AITTYMGVLHTNLTANFWQPNFTMRLTRYAPPPNQGIVPQTVIVLLASVRVSSSGGVY
                     YLDRNGSWTNGQVTLRLPFQSPAYKAPTLAWQAGAFAWATWAADFSEGPSGQSLDGGM
                     ARECWVFEYVQDTDRFDGGHILIRNSANPGDWWHTYNENVRLTAGPVRIGMAGCRQIL
                     NFAPIPYADSTPNAVSCWAKELKPLPGASYEDTASWAALTTDAANWTVTVADKDDATV
                     QAAIGYRPAVTFTPTVSLDTTRPVLWQVAEDHPATIAVPAGLPAAEDTDGDAALVDLS
                     LHFDDTWRNAGGTATFKRSDTALYPDWLERGKVECHLGWQPDPDPGADMVDQNVATLW
                     IAPGGIVRDRRGEEQLGAPGLRVELADLVRVKLQESCIVDLGEASGVTAGTWFDTCGN
                     RMGLPATMIDVAAAIAAQVLPSAEPCPSTPHLKPQDGQSWEQHLDEVCKASGLRWGYN
                     PHTVKLFLDGGRPEYVPGTSTISFVLDYDTLLPDQTPFDPTHIRDNSGHRNRYKILYG
                     PEDNRKAYYWTADSTEIQADGGDYWAVADETNGDTVAEMQADLDDQHRTRQSLIVFEI
                     PLRVGLRCEQFVQIGDCPEMGLEVGAIYQISELDHSRSGRRTRVAAKRVYSPGGYY"
     gene            3334..>4435
                     /locus_tag="PLQ54_04015"
     CDS             3334..>4435
                     /locus_tag="PLQ54_04015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS92452.1"
                     /translation="MPRVFGQPRKAERDSGAAVVVGPRQIVPGRRGSSACRRVAFVTP
                     GYYLGGVETVNCALADGLPRDEFAADVVCYTSTEETVAADADHWDRIVTLGPVETYGR
                     PCPDALSDLLEWVLRDYALVVAALCCEPLRAAHKLGLPAIEYWHGVCGWNSMDKPAAR
                     ILAVSRDTLDATRARRMGLLPPCEVLYNGLDVQRFSSLRRDPGAAGFGLRGAEKVLLY
                     CGRLSHEKRPEDAIAVAGLLHRRNPAWKLLLAGEVYSYNREHYLRLMQAAGLVPGRDV
                     AWVSLARDQVARAYAAADCLLHPSLEEGFGMVLAEALAAGVPIVTTGVGGSGEVVGEA
                     ALRVPVRDTAAMAEAVASIFTDDATAGWLAQAG"
BASE COUNT          742 a         1499 c         1540 g          654 t
ORIGIN      
        1 cgacgagagc aacttcctgg gcttcctcta cccgtgggac ttgtgccgcg agacctctga
       61 tgacaacagc ctcaaagcgg ccattcatgt cagcgaactg gacttgatct cgtgcccctg
      121 cgcgccgatc acgttcaacg cggtatgggt cacacacggg cgcgcgcatg gcatggcctt
      181 ccaggagggc gggggctttg tgcggggggg cggcaccagc agcgatgatg cgacagcagg
      241 gacagtcgcg ttcttccgca atgccaacgg ggccagcggt tgggagacga tagggagctt
      301 cgagcctgac gcgacggggc gctggcggac acccccggcg aaggaactgg gctataccta
      361 cggcgtcaag catcgcagca tggcgtcgca cgatgacatg ggcgagttcc gcacccgcga
      421 gtatcagtac ggcgaagtga cagtgacgga ggagttgctg ctggggtgct gctccattgc
      481 ccgcgatgcg atgggttgcc cgatctgggc cgcctgcgag ctggagggca cggtcaatgc
      541 ctacgccctc aacgacttcg acttctggca ctgccccggc aacccggccc tcaccggggc
      601 cgcctacaag tacgcctcga tctgcgcctg tgacgacggc tcgatcatcg tgggggccac
      661 caacacggac caggggcgca ttgacgtggt gcgctcccgc aacaatggca ccacctggga
      721 ggcggtcaac acggccttcg gcaccggact tgtcaacggg cacctgttcc atatccacgg
      781 acaggtctac ctcgtgggct gggccagcga caaggtgtgg ctcgtcacga gcagccaggc
      841 ggaccttacc cgcgaggtca tggacggcac gaacaccatc ctggaggtct gcgctcatgc
      901 cgtgggcgcg ggcgaggacg ccccgcaatg cagcgtcatc attgacgaca cggggcagat
      961 catcgtggca gtcgccgggg acgccgacac cagattctac tcctgccgca acttccgcac
     1021 cggcttctcg ctcgtcgcct gagccgcacg acgccgccga atatgaccgg ccacccacat
     1081 ccaggggtgg ccgctttgat tccgccggga gcatggcatg agtctcattc gcaccgaagg
     1141 gagcctggtc gtctaccatc ccgccgcaga cgggattccg acggtgcagg gcttctcgcg
     1201 cgactggtac ccgagcgcca agtgggagag cgcggggggc tgcgagttca tcaagccaat
     1261 caatgccatc gcgccggccg gggacctgcg caccttggcg atgaccgcca ccttcgcgat
     1321 tgacgccagc tacaaccagt cgctacgcca gatcatcacg ccggggaacc ccccggtcgc
     1381 gagctacgag ttctatgaca actccgccta cgccagcggc acggccatta ccacctacat
     1441 gggcgtgctg cacaccaacc tgaccgccaa cttctggcag ccgaacttca ccatgcggct
     1501 gacgcgctac gcgccaccgc ccaaccaggg gatcgtgccg cagacggtga tcgtgctgct
     1561 cgccagcgtg cgggtcagca gcagcggcgg ggtctactac ctggaccgca acggcagttg
     1621 gaccaacggg caagtcaccc tgcgcctgcc cttccagagc ccggcctaca aggcgccgac
     1681 gctggcctgg caggcgggcg ccttcgcctg ggcgacctgg gccgccgact tttccgaggg
     1741 ccccagcggc cagagcctcg acggcggcat ggcccgcgag tgctgggtct ttgagtatgt
     1801 gcaggacacc gaccgctttg acggcgggca catcttgatt cgcaacagcg ccaacccggg
     1861 cgactggtgg cacacctaca acgagaacgt gcgcctgacg gcggggccgg tgcgcatcgg
     1921 gatggcgggc tgccggcaga tcctcaactt cgcgcccatt ccctacgccg acagcacccc
     1981 gaacgcggtg agctgctggg ccaaggaact caagcccctg cccggcgctt cctacgagga
     2041 caccgcgagc tgggcggcgc tcaccaccga cgccgccaac tggaccgtca cggtcgccga
     2101 caaggacgac gcgacggtgc aggcggcgat tggctaccgc ccggcggtca cgttcacgcc
     2161 cacggtctcg ctcgacacga cgcgcccggt gttgtggcag gtggccgagg accatcccgc
     2221 caccatcgcc gtgccggcgg ggctaccggc cgccgaggac accgatgggg acgcggccct
     2281 ggtggacctc agcctgcact ttgacgacac gtggcgcaac gccgggggca cggccacctt
     2341 caagcgcagc gacaccgccc tgtatcccga ctggctggag cggggcaagg tggagtgtca
     2401 cctgggctgg cagcccgacc ccgaccccgg cgccgacatg gtggaccaga acgtcgcgac
     2461 cctgtggatt gcgccgggag gcatcgtgcg cgaccggcgc ggcgaggagc agctgggggc
     2521 gccgggcctg cgcgtcgagt tggccgacct ggtgcgcgtg aagctgcagg agagctgcat
     2581 cgtggacctc ggcgaggcct ccggggtgac ggccgggaca tggttcgaca cctgcggcaa
     2641 ccgcatgggc ctgccggcga cgatgattga cgtggcggcg gccattgccg cccaggttct
     2701 gccctcggca gagccgtgcc ccagcacccc gcacctcaag ccccaggacg gccagagctg
     2761 ggagcagcac ctggacgagg tgtgcaaggc cagcggcctg cgctggggct acaacccgca
     2821 cacggtcaag ctattcctgg atggtggccg cccggagtat gtgccgggca cgagcacaat
     2881 cagcttcgtg ctcgactatg acacgctgct gccggaccag acgcccttcg acccgacgca
     2941 catccgcgac aacagcgggc accgcaacag gtacaagatc ctctacggcc ccgaggacaa
     3001 ccgcaaggcc tactactgga cggcggactc cacggagatt caggcggacg gcggcgacta
     3061 ctgggccgtg gctgacgaga ccaacggcga cacggtagcg gagatgcagg ccgacttgga
     3121 cgaccagcac cgtacccggc aaagcctcat cgtctttgag attccgctgc gcgtgggcct
     3181 gcgctgcgag cagttcgtgc agatagggga ttgtccagaa atgggtctgg aagtgggtgc
     3241 catctaccag atcagcgaac tggaccactc ccgcagcggt cgccgtacca gggtagcggc
     3301 caagcgcgtc tactcgccgg gagggtacta ctgatgccgc gtgtcttcgg gcaaccgcgc
     3361 aaggcggaac gcgacagcgg cgcggcggtg gtagtgggac ctcgccagat cgtgccgggg
     3421 cggcggggat cctcggcgtg ccggcgtgtc gccttcgtga cgccgggcta ctacctcggc
     3481 ggcgtggaga ccgtcaactg cgccctggcc gacggcctgc cgcgcgacga gttcgcagcc
     3541 gatgtcgtct gctacacctc gaccgaggag actgtcgcgg ccgacgccga ccattgggac
     3601 cggatcgtca cgctggggcc ggtcgagacc tacgggcggc cctgcccgga cgcactgagc
     3661 gacctgctgg agtgggtgct ccgtgactat gcgctggtcg tcgccgccct gtgctgcgag
     3721 ccgttgcggg cggcccacaa gctgggcctg ccggcgattg agtactggca tggggtgtgc
     3781 ggctggaaca gcatggacaa gccggcagcg cgcatcctgg ccgtgtcgcg ggacacgctg
     3841 gacgccaccc gggcgcggcg catggggctg ctgccgcctt gcgaggtgct gtacaacggt
     3901 ctggacgtgc agcggttctc cagcctgcgg cgcgatcccg gggccgctgg ctttggcctg
     3961 cgcggcgcgg agaaggtgct gctgtactgt gggcgcctga gccacgagaa gcggccggag
     4021 gacgccatcg ccgtcgccgg actgctgcac cgccgcaatc cggcctggaa gctgctcctc
     4081 gcgggcgagg tctactccta caaccgcgag cactacttgc ggctcatgca ggccgccggt
     4141 ctcgtgccgg ggcgagacgt ggcctgggtg tcactggcgc gcgaccaggt tgcccgcgcc
     4201 tacgcagcgg cggactgcct gctgcacccg tcgctggagg agggcttcgg catggtgctc
     4261 gccgaggcgc tggctgccgg cgtgcccatc gtgacaacgg gcgtgggagg cagcggggag
     4321 gtcgtgggcg aggcggcgct ccgggtgccc gtgcgcgaca cggcggcaat ggccgaggcc
     4381 gtagccagca tcttcaccga tgacgcgacg gcggggtggc tggcacaagc cggac
//
LOCUS       DAOSPL010000151         4434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_19737_length_4434_cov_3.343686, whole genome shotgun sequence.
ACCESSION   DAOSPL010000151 DAOSPL010000000
VERSION     DAOSPL010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19737_length_4434_cov_3.343686"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="PLQ54_04020"
     CDS             <1..1372
                     /locus_tag="PLQ54_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92453.1"
                     /translation="CLDDASERVFPWGLFYPNDLVPKDVALTYRNIGLLFRLLTPKYE
                     PPRVTVLLPDRHRLGPQSGRIHDAIWRTFATLTSLRIRYNTLNEGALDVLPAATRVLV
                     YPVPYCLDDATYSRLLAFVRQGGALYVSGDVSYGPDRTRTQTGRLRELVGMAFGEELY
                     PNIGYDAAAPKASAPTGSGQALGLVPFAAAAGIRFGELATGAGGPPEVLQVTQDGEHI
                     PVVVARGLGKGRVFFVNDPVELRTDLADDVLRSIHAAFATYAEVDRLQIEPDVPDVHV
                     FRVPVEGAEEVVVAVNRGEARTVRLALGTHRVELALARAGTGMIWLDSEGRAVAVEAQ
                     GEVQVDGRRVADLPCNAAVAALDGHGLLESSSVLILPFGEGVLRVESSRDWRDRVVEV
                     GDFEDGQWRPLESLECYTQPHLNVVLDGDQAASLLFVGERGELEASRRRLSALVQRPD
                     LSLASP"
     gene            1664..2764
                     /locus_tag="PLQ54_04025"
     CDS             1664..2764
                     /locus_tag="PLQ54_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOS92454.1"
                     /translation="MAGGRGARLYPLTQDAPKPLLPMMGRPLIDHTMAYLERQGFREA
                     IITTGHQCKPLLNHLNRTSYHPCDVVCALEEFPLGTAGSVRNAESMLSETFLVMSSDI
                     VTDADLMEAVRAHRRSGALMTMLVSEVPDVSLFGMVVADAKGRVIDFREKPRSPDEVV
                     SRLANAGIYVMEPEALGMIPRSRYCDFARDVIPRMLASGRSVYAHPISGYWCDVGTAQ
                     RYRKAHLDVMKGRVQLPGFDAPSAGADGAASGATLDPSVKISGPVWVGRGASVGAGTR
                     LGPYAFLADGVHVGDGASIAWSVLNQGVVVGSSSQVDRSVIGSDTRLPSGSRVRSSVI
                     HGYTTIRIPATDSEPMAPLSRSAEGMRTAAVT"
     gene            2959..3600
                     /locus_tag="PLQ54_04030"
     CDS             2959..3600
                     /locus_tag="PLQ54_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92455.1"
                     /translation="MSDSVPLEQLQQLWDRTVEELRKGEVNLPLWEAAETCVALALED
                     DVLVLGIRSEDYHRAGHLKAPVAAHQIRAILERLAQRSLSLEVIEGTTPKHWADEKLR
                     RQQREDTARRALEKTKVDTERELGSWDEVSTHLAQVYNTLKDRNLPWVAAQYLLDSLV
                     TVAKVEWRMRQAPDADTHNIDRGVARVLHALSGRVDVPLNVVGLEYVRVKRWQ"
     gene            3657..>4434
                     /locus_tag="PLQ54_04035"
     CDS             3657..>4434
                     /locus_tag="PLQ54_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92456.1"
                     /translation="MAGRQLWALAMWVALGLPAVGQTYDLAERYAQGYEVLVTSSSVV
                     DSKVTGRGPKALEDAIARMSGGLSVTQTGQVRYRVREAVAGRPVQQEAVVLRGETRAP
                     GAPASAQVSHSKIGGVPVLVRAPRGGAKLYRGKSGGQIDQAEAARWCDDLPAELLEGE
                     DVSASRIGDSWTIKGDDLCQRLGLPEGSDGRIELTFEAVVEKEGKRLAQLKVAWTMSS
                     APAAGKAPLSGMKMDLRGYYFIDLETHLPYYLDLHGSSTMD"
BASE COUNT          700 a         1404 c         1567 g          763 t
ORIGIN      
        1 gtgcctcgac gatgcctccg agcgcgtgtt cccctggggg ctgttctacc ccaacgatct
       61 cgtacccaag gatgtcgcac tgacctaccg caacatcggg ctgctcttcc gcctgctcac
      121 gccgaagtac gaaccgcccc gggtgaccgt gttgctgccg gaccgccacc gtctgggtcc
      181 gcagtcgggg cggatccacg atgccatctg gcgtactttc gccacgctga cgagtcttcg
      241 catccgctac aacacactca acgagggagc cctcgatgtg cttccggctg cgacgcgggt
      301 tctcgtctac cccgtgccct actgcctcga cgatgcgacc tactcgcggc tgcttgcctt
      361 cgtcaggcag ggaggggcgc tttacgtctc cggcgatgtc tcctacggcc cggaccgtac
      421 gcgcacgcag accggccgcc tgcgcgagct cgtcggtatg gccttcggcg aggagctcta
      481 cccgaacatc gggtacgacg ctgccgcccc aaaggccagc gcccccacag gcagtgggca
      541 ggcgctgggc ctggtcccgt tcgcggcggc ggcaggcatc cgcttcgggg agctcgcgac
      601 cggagctggg ggcccgcccg aggtgctgca ggtgacccag gatggcgagc acattccggt
      661 ggtcgtcgcc cgcgggcttg ggaaggggcg cgtgttcttc gtcaacgatc ctgtcgagct
      721 gcgcaccgat ctggccgacg acgtgctgcg gtcgatccat gcggcgttcg ccacctatgc
      781 cgaggtcgac cggctgcaga ttgagcccga tgtacccgac gtccacgtgt tccgcgtgcc
      841 ggtcgaaggg gccgaagagg ttgtggtggc cgtgaatcgc ggcgaggcgc gtaccgtgag
      901 gcttgcgctg ggaactcacc gcgtcgaact cgcgttggca agagcgggca cgggtatgat
      961 ctggctcgac agcgagggcc gcgccgtggc cgtggaggcc cagggcgagg tacaggtcga
     1021 tggacggcgc gtggccgatc tgccctgcaa tgcggcggtc gccgccctgg acggtcatgg
     1081 gttgctggag tcgagctcgg tcctgatctt gccctttggc gagggggtgc ttcgggtcga
     1141 atcctcccgc gactggaggg accgcgtggt tgaggtgggc gatttcgagg acgggcagtg
     1201 gcgtcccctg gagagcctcg agtgctacac ccagcctcac ctcaacgtgg tgctagatgg
     1261 cgatcaggct gcctcgctgt tgttcgtggg agaacggggt gagctggagg cgtctcggcg
     1321 acggctgagc gctctggtgc agagaccgga cctgtcgttg gcgtctccat agatgcccgt
     1381 cgcggggcgg caaaaactgt gtgaattccc ggtgtactcg gaggatcgac ctggaggccg
     1441 tggaaagggg attggcttca gccgaggatc attctcgatg ccggtgatct gtggatcaag
     1501 gcgatctggg ggctcggctc ttcgacttct cgcctcggga ggaatggacc cacttcaggc
     1561 cctggcagtc tcgacaggtc tgcccgccca gttgccacac cgtgagcttc cgtctccaag
     1621 cacggctacc gcctaggggg gtggtcacgt gaaggcggtt gtcatggccg gaggtagggg
     1681 ggccaggctc tacccgctga ctcaggatgc acccaagccg ctactcccga tgatgggtcg
     1741 gccacttatc gaccacacca tggcgtatct ggagcggcaa gggttccgtg aggccatcat
     1801 cacgacaggg caccagtgca agccactcct gaaccatctc aaccgcacca gctaccatcc
     1861 gtgtgacgtc gtctgcgcgc tcgaggagtt cccactcggc acagccggaa gtgtgcggaa
     1921 cgccgagagt atgctctcgg agaccttcct ggtgatgtcg agcgacattg tcaccgatgc
     1981 cgatctcatg gaggccgtgc gcgcccatcg ccggagcggg gctctgatga ccatgctggt
     2041 gtccgaggtc cccgatgtct cgctctttgg tatggtggtt gccgacgcga aagggcgcgt
     2101 gatcgacttc cgtgagaagc ctcgctcgcc cgacgaggtg gtcagccgcc ttgcgaacgc
     2161 cggcatatac gtcatggagc ctgaggccct ggggatgatc ccgcgctcgc gctactgcga
     2221 cttcgcgcgc gacgtgatcc cgcggatgct ggcgagtggc cggtcggtct acgcgcaccc
     2281 gatctcagga tactggtgtg atgtgggcac ggcgcaacgg taccgcaagg ctcacctcga
     2341 cgtgatgaag ggccgcgttc agttgccggg gtttgacgcg ccgtcagctg gtgccgacgg
     2401 cgccgcgtcc ggggcgaccc tcgatccctc ggtcaagata tcgggcccgg tctgggtggg
     2461 tcgaggggct tcggttgggg ccggcacgcg gctggggccg tatgcgttcc tggcggacgg
     2521 ggtgcatgtg ggcgacggcg cgtcgatcgc ctggagcgtg ctcaaccagg gcgtcgtcgt
     2581 tgggtcctcg tcgcaggtgg accgatcggt catcgggagc gatacgcggc tgccaagcgg
     2641 gtcacgggtc cgctcgtcgg tgatccacgg ttacacgacg atccggatcc cggcgacgga
     2701 ctcggagccg atggcgccgc tgtcgcgttc tgcggagggg atgcggaccg ccgcggtgac
     2761 ataggggctc tcgcgcgcag tggtgatgac acgcccgggg tgcagtctcc ccgggcgtgt
     2821 ctggtctgcc tcccagcacc tcgttggtcg ccagggatgg cgccaggcag gaactcggcc
     2881 cgcgaaccgc caatccccct gtcatcctat tgggccgggg gccctgctgg ccccccatcc
     2941 ggccggagga ggcacctggt gagcgattcc gttccactgg agcaactgca gcagctatgg
     3001 gaccgcacgg tcgaggaact gaggaagggc gaggtcaacc tcccgctctg ggaggcagct
     3061 gagacgtgcg tggccctggc cctggaggac gatgttctcg ttcttggcat ccgctcggaa
     3121 gactaccatc gggcgggtca cctgaaggca cccgtggccg cgcaccagat ccgcgccatc
     3181 ctcgagcggc tggctcagcg gagtctgagt ctcgaggtca tcgagggcac cacacccaag
     3241 cattgggccg acgagaagct tcgccggcag cagcgcgagg acacggcccg cagggcgctc
     3301 gagaagacaa aggtcgacac cgaacgcgag ctgggatcat gggacgaggt ctcgactcac
     3361 ctcgcccagg tctacaacac actcaaggac cggaacctgc cctgggtggc ggctcagtac
     3421 ctcctggact ccctggtcac ggtggccaag gtcgagtggc gcatgcggca ggcccccgac
     3481 gccgataccc acaacatcga ccgaggcgtg gcacgggtcc tccatgcgct atccggccgg
     3541 gtcgacgtgc ctctgaacgt cgtcgggctc gagtacgtgc gggtgaagcg ctggcagtag
     3601 gacgagcacg acgcgcgccg gagaccatcc agccaaggcg aagggcaggc gaaaacatgg
     3661 caggtcgaca gctctgggcc ctggcgatgt gggtcgcgct cggtcttccc gcggtcggtc
     3721 agacctatga cctggccgag cgctatgccc aggggtatga ggtgctggtg acctcgagtt
     3781 cggtggtcga ctcgaaggtc actggtcgtg ggcccaaggc actcgaggat gccattgcac
     3841 gcatgtccgg cggcctctcg gtgacacaga ccggccaggt ccgttaccgg gtgcgcgagg
     3901 cggtcgcggg gaggcctgtc cagcaggagg cggttgtcct caggggggag acccgtgctc
     3961 ccggcgcccc ggcctcggcc caggtctcac acagcaagat cgggggcgta ccggtcctcg
     4021 tccgggcgcc gcgcggcggg gccaagttgt accggggcaa gagcggtggc cagatcgacc
     4081 aggccgaggc cgctcggtgg tgtgacgatc tgccggccga gctgctggag ggtgaggacg
     4141 tctcggcatc gcgcatcggc gactcgtgga ccatcaaggg cgacgacctg tgccagcgcc
     4201 ttgggctgcc cgaggggagc gacggacgga tcgagctcac gttcgaggcg gtggtcgaga
     4261 aggagggcaa gcgcctcgcg cagctcaaag tggcatggac catgtcgagt gcccccgccg
     4321 cgggcaaggc cccgctgtct gggatgaaga tggatctgag aggctactac ttcattgatc
     4381 tggagacaca tctcccctac tacctcgacc tgcatggcag ctccaccatg gacc
//
LOCUS       DAOSPL010000152         4417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_19835_length_4417_cov_3.521320, whole genome shotgun sequence.
ACCESSION   DAOSPL010000152 DAOSPL010000000
VERSION     DAOSPL010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19835_length_4417_cov_3.521320"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2944)
                     /locus_tag="PLQ54_04040"
     CDS             complement(<1..2944)
                     /locus_tag="PLQ54_04040"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOS92457.1"
                     /translation="MTRTIHLALALTFCLALEVQAALLREAISLNGEWDVQVVPALGL
                     PVTDAWGTVTVPGTISGTDGRCAWFRTRFTIPTSMAGRRLLLHFGGVKYNSRVLVNGQ
                     HVGGHFGGYDPFDVDITAVAWVGEENELLVGCRDWTGIFSHPVDFAKAPADWDSLRAY
                     PKDAILAPIGGIFSEFGPWDDVELRAQPFVYVDRVAITTSVRQRRIDVEYTLRNATPD
                     AITVDLAARVEDPSGPQGLRAEGIRIRPGGSARVQAGAPWPNARLWSHEDPQLYHLVT
                     ELRRGNEDVDALRTRFGFREFWVDGAELYLNGSKVHLLASSAWPMHSLKDRDAVRDFW
                     TRIRAGNNIAFRTHTQPWRRVFYDVADEVGILVIPEGAVWNDDESYRVNDPAFWENYA
                     EHLRAMVEQYRNHPSVVMYSLENEMYGARQNERNVFATEQLAELGRRVKRWDPTRPIT
                     YESDGDPLGVADVVGIHYPHEYPDFTQYPNTGWWLEDGTRGVGQYGGKTDGLWRWDRL
                     KPLYIGEFLWVPSPDPSWDTVFVGDQAYSDPPRYHNLAKAQAWSMQIEAYRDQGVNGI
                     CPWTLVEGGALDDTNPLYVAQRQAFRPIAAFPREYNARFYADDEVERNLTVYNDILSP
                     STLVLTWELLLDGQRAASGHERLNMAPGERMHTRIRFRVPRVAVRTPLELVLKIHRDG
                     ELRFEEHHPGTAWPRGGIAVPRGSRVVVYDPAGGLDGVIGPDVPRIASLQSVPAPPAV
                     LVVGAEAFPPPPQGRQRLGVLSNRDQAMVEFVRQGGRVIVLHQRAYPSWIGTGRLTSH
                     ASTMTFAQMPDHPALRGITDRDLKWWASPPGAPADHLVTGQECARPESGAFRAIVVSG
                     SASGIAHAPLLELPCGQGTVLLCQMLVQEKSAFEPVAAQLLTNLLDYAANYSPAPRQA
                     VLVAGSDEAAAALTDAGLRYTDQRLSRPPAGLERPGLAIADGPTADGAALHALAASGG
                     TVVLHCPSP"
     gene            3047..3892
                     /locus_tag="PLQ54_04045"
     CDS             3047..3892
                     /locus_tag="PLQ54_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOS92458.1"
                     /translation="MAVRTVPKIEPPFPGLGEIVAVMGEAGHRLAEIGASEGAAGNIS
                     VYIGWPLDPRRRFPVEEEIELPEPAPELSGGLLLVTGSGRRLREIAQDPEANIGALIV
                     NPDGRTGRFYASHRRLFARFTSELNSHLAVHRQQVPADDLNFHAVIHGQPIHLTYLSH
                     LPEYQDSLYLNRHILRWQPELIVNLPEGVAALPFRVPGSPELMELTLAAMRDHRVVVW
                     GKHGVMARSALSVKRAADLIEYAETGARYEYLNLANHERAQGLAVEDIQAICRAFSIE
                     QSIFG"
     gene            3919..>4417
                     /locus_tag="PLQ54_04050"
     CDS             3919..>4417
                     /locus_tag="PLQ54_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOS92459.1"
                     /translation="MAPTRASRGLALAAVLLVAGSALAQQAPEPTIQVLDNGLTVIVQ
                     EQHASPVVAVRFLVGTGSAYEGRYLGAGLSHLVEHAIDGGTPTRSRTDIQKLIESIGN
                     ASNAMTEYDTTQYYITTSAPCWSQALEVLADYVLHPTFPEEIVQQEQGVIRREMAMSD
                     DNPADR"
BASE COUNT          750 a         1417 c         1565 g          685 t
ORIGIN      
        1 cgggcgaggg gcagtggaga acgacggtgc cgccagaagc cgccagggcg tgcagggccg
       61 cgccatcggc cgtcggtccg tccgcgatgg ccagcccggg acgctcgagg ccggcgggtg
      121 ggcggctcag acgctggtct gtgtagcgca agcctgcgtc ggtcagcgcc gccgcggcct
      181 cgtccgaccc ggcaaccaga acggcctggc gaggcgccgg cgagtagttg gctgcgtaat
      241 ccagcaggtt ggtcagcagc tgggcggcca cgggttcgaa ggcgctcttc tcctggacga
      301 gcatctggca cagaagcacg gtgccctggc cgcagggcag ctcgagcagc ggtgcatgcg
      361 cgatgccgct ggcgctgccg gacacgacga tggcccggaa ggcccccgac tcgggacgag
      421 cacactcctg gcccgtgacg aggtgatcgg cgggcgcgcc ggggggtgag gcccaccact
      481 tgaggtcgcg gtcagtgatg ccccgcaggg cggggtgatc cggcatctgc gcgaaggtca
      541 ttgtcgaggc gtggctcgtg aggcggccag tgccgatcca gcttggatag gcccgttggt
      601 ggagcacgat gacgcgcccg ccctggcgga cgaactcgac catggcctga tctcggttac
      661 tcaggacgcc cagccgctgg cggccttggg gcggcggcgg gaaggcctcg gcgccgacca
      721 cgagcaccgc gggtggcgcg ggaaccgact ggagcgacgc aatgcgcggg acgtcggggc
      781 cgatgacccc gtcgagcccg cccgcggggt cgtatacgac gacgcgggac cccctcggaa
      841 ccgcgatgcc gccgcgcggc catgcggtgc ccgggtggtg ctcctcgaag cgcagttcac
      901 cgtcgcgatg gatcttcagc accagctcca acggggtccg gacggcgacg cgtggcacac
      961 ggaatcggat ccgcgtgtgc atgcgctcgc cgggcgccat gttgaggcgc tcgtgccccg
     1021 aggcggcgcg ctgtccgtcg aggaggagct cccatgtgag caccagggta gagggcgaga
     1081 ggatgtcgtt atagacggtc aggttccgtt cgacctcgtc gtcggcgtag aagcgggcgt
     1141 tgtactcacg agggaaggcg gcgatggggc ggaaggcctg ccgctgagcc acatagagtg
     1201 gattggtgtc atcgagagca cctccctcca cgagcgtcca tggacagatg ccgttcacgc
     1261 cctggtcgcg gtaggcctcg atctgcatcg accaggcctg agccttggcc aggttgtggt
     1321 accgaggcgg gtccgagtag gcctgatcgc ccacgaaaac cgtgtcccac gacggatcgg
     1381 gcgacgggac ccacaggaac tcaccgatgt acaggggctt gagccggtcc cagcgccaca
     1441 ggccgtccgt cttgcccccg tactgcccga ctccgcgggt tccgtcttca agccaccaac
     1501 cggtattcgg gtactgggtg aagtcgggat actcgtgcgg atagtggatc ccgaccacat
     1561 cggccacgcc cagagggtcg ccatcggact cgtaggtgat gggtcgcgtg gggtcccagc
     1621 gcttcacgcg ccggccgagc tcggcgagct gttcggtggc gaagacgttg cgctcgttct
     1681 gccgagcgcc atacatctcg ttctcgaggc tgtacatcac cactgatggg tggttccggt
     1741 actgctcgac catggcgcgg aggtgctcgg catagttctc ccagaatgcc ggatcgttga
     1801 cgcggtacga ctcatcgtca ttccagacag cgccttcagg gatgaccagg atgccgacct
     1861 cgtcggcaac atcgtagaac acgcggcgcc agggctgcgt gtgagtgcgg aaggcgatgt
     1921 tgttccccgc ccggatgcgt gtccagaagt cgcggacggc gtcgcgatcc ttcagcgagt
     1981 gcatgggcca ggccgagctg gccagcaggt gaaccttcga gccgttgagg tacagctcgg
     2041 ccccgtcgac ccagaactcg cgaaagccga agcgagtccg gagcgcgtcg acgtcctcgt
     2101 taccgcggcg aagctcggta acgaggtgat atagctgggg gtcctcatgg gaccaaagac
     2161 gggcattcgg ccacggtgca ccggcctgga cgcgagccga gcctccgggg cggatccgta
     2221 tgccttctgc cctcaggccc tgggggcccg atgggtcctc cactcgggcg gccaggtcga
     2281 ccgtgatggc atcaggggtg gcgttgcgga gcgtgtactc gacgtcgatg cgccgctggc
     2341 ggacggacgt ggtgatggcg acgcgatcga catacacgaa gggctgggcc cggagctcga
     2401 cgtcatccca cgggccgaac tcgctgaaga tgccaccgat gggggcgagg atggcgtcct
     2461 tcggatacgc tcggagcgaa tcccagtctg ccggagcctt tgcgaagtcg actgggtggc
     2521 tgaagatgcc ggtccaatca cggcatccga cgagaagctc gttctcctcg ccgacccatg
     2581 ccacggcggt gatgtcgacg tcgaacgggt cgtagccgcc gaagtggccc ccgacgtgct
     2641 gaccgttcac gagcacgcgg ctgttgtact taacgccacc gaaatgcagc agcaggcggc
     2701 ggcctgccat actcgtgggt atggtgaacc gcgtgcggaa ccaggcacac cgaccgtccg
     2761 tccccgagat ggtgcccgga acggtgaccg ttccccatgc atcggtgacg ggcagaccga
     2821 gtgccgggac cacctgcacg tcccactcgc cattgagcga gatcgcctcg cgcaagaggg
     2881 cagcctgtac ctccagcgcg aggcagaagg tgagggcaag agccaagtgg atggtccgtg
     2941 tcatggcagg tccctcccgg gggtgtggga actgaagggg gattcccccg ggtggcccag
     3001 gctcctgccg caagacgagt ggcccggcga tgagtgaggg agagcgatgg ccgtgcgaac
     3061 cgtgcccaag atcgagcccc cgttcccggg gctaggcgag atcgttgccg tgatgggtga
     3121 ggccggccat cggctggcag agattggagc cagcgagggg gccgcgggga acatctcggt
     3181 ctacatcggg tggccccttg acccccggcg gcgcttcccg gtcgaggagg agatcgagct
     3241 tcctgaaccc gctccagagc tcagcggggg gctgctgctg gtaacgggat cgggccgacg
     3301 gcttcgggag atcgcccaag acccggaggc caacatcggc gcgctgatcg tgaaccccga
     3361 cggtcgaacg gggcggttct acgcgtcgca ccgacggctg ttcgcccggt tcacgagcga
     3421 gctgaactcg cacctggccg tgcatcggca gcaggtaccg gccgacgacc tgaacttcca
     3481 cgccgtcatc cacggccagc cgatccacct gacctatctg agccatctgc ccgagtacca
     3541 ggacagcctc tacctgaacc ggcatatctt gcgctggcag ccggagctca tcgtgaatct
     3601 gcccgaaggg gtggccgcgc tacccttccg cgtcccaggg tcccccgagc tgatggagct
     3661 cacgctggcc gccatgcgtg accaccgtgt cgtggtgtgg ggcaaacacg gcgtgatggc
     3721 gcggtcggcg ttgtcggtga agcgcgcggc cgacctgatc gagtacgccg agaccggtgc
     3781 ccgatacgag tacctcaacc tggcgaacca cgaacgagcc cagggtttgg ccgtcgagga
     3841 catccaggcc atctgccggg cgttctcgat agagcagagc atcttcggct gacaccgaca
     3901 cgagggggaa ccactcctat ggctccgaca cgggcttcgc gaggactcgc gcttgccgct
     3961 gtcctgctgg tggccgggtc ggcactggcc caacaggcgc ccgagcccac gatccaggtg
     4021 ctcgacaacg ggctcacagt catcgtgcag gagcagcacg cgagtcccgt ggtggcggtc
     4081 cggttcctgg tggggacggg gagtgcctac gagggccggt acctcggggc cgggctctcg
     4141 cacctggtcg agcatgccat cgacggcggc acgccaaccc ggagccgcac cgacatccag
     4201 aagctgatcg agtccatcgg gaacgcgtcc aacgcgatga ccgagtacga cacgacgcag
     4261 tattacatca ccaccagtgc tccgtgctgg agccaggcgc tggaggtgct ggccgactac
     4321 gtccttcatc cgaccttccc cgaggagatc gtccaacagg agcaaggggt aatccggcgt
     4381 gagatggcca tgtcggatga caatcccgcc gaccgcg
//
LOCUS       DAOSPL010000153         4416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_19840_length_4416_cov_3.345563, whole genome shotgun sequence.
ACCESSION   DAOSPL010000153 DAOSPL010000000
VERSION     DAOSPL010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19840_length_4416_cov_3.345563"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1346)
                     /locus_tag="PLQ54_04055"
     CDS             complement(9..1346)
                     /locus_tag="PLQ54_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92460.1"
                     /translation="MGGPAETEGATEAETAPERPPPADSPKATVDTAQAEQMSQRLGE
                     SRLRVLEESISAAAAASGTEPQPSPQAQVDPTALKILAQISRVELAACRRALAARQVE
                     LADARAVHRELVSRLRREPRDVRAGAKLVRDARLRVEALGERGLQYESVFESLQQAYV
                     AIQRRLARYDRLLADARAVTGPSWDDAASQGSGTLGRLRRETITNLDRCRSTAEQTRQ
                     VLLESMSTVSENVELASDLALELESAIRQAGERSLLRQGATPLSAQSWLSLGTAVWLG
                     VRSLASALVHPDDRLASIGSQWQASRDSADAVHPRALFVTGVLLAAVLALSVIRRFAA
                     SGGPGGGSADDRHWRIRVGARSLVALTVAGSMGALSWGVCRPVYDLWLLTTAASLVAA
                     LVAWRWFLRVAIAPDCEWRLWLPDRDTSEAYRIYLAAGWLGALALLRRRPGRR"
     gene            complement(1807..3582)
                     /locus_tag="PLQ54_04060"
     CDS             complement(1807..3582)
                     /locus_tag="PLQ54_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOS92461.1"
                     /translation="MRNAAYTAEHVRSAAGPLLPAIASLHGSPSARPLLYDVISRCQW
                     HTYEAVPHTELGHTVRDCGRALCSMISERSEALSGFSVFSALLDVAARQDRPDLQPGF
                     HAEMVHLLEGLQGKACLGPLAEDPSGEDPGSAAQRSVALDALWTSAASWMERYEDGLS
                     PEAVARRSVRRAEVLAALGGDEADWADWQWHAAHVIAGLDSLDRAVALRADEREGVRR
                     ATAEGMPFAVTPYYASLFDEVADSGRDRALRAQVLPSCQCASAWGEHMRDGGPGAMDF
                     MREGETSPIPLVTRRYPAVAILKPYRSCSQFCVYCQRNWEVTGPGSCEALAPWEQIEA
                     ACDWIAAHPAIQELLITGGDPLAMPDDALARILRRVRGIASLNVVRIGTRIPVTLPMR
                     ITHSLADLLGSLRSPGHREILLVTHVQHPYEVTPEMVAAIARLRSRGIGIYNQVVYTF
                     YASRRFETARLRMLLRRVGVDPYYTFAPKGKAETLDYRVPIARILQEQKEEARLLPGT
                     RRTDEAVVNIPALGKVYLRAAQHRDLLAVLPNGSRVYEFHPWEKGIAPSQPYVSVDVP
                     VLDYLARLESIGEDPAEYGSIWYYF"
     gene            complement(3831..>4416)
                     /locus_tag="PLQ54_04065"
     CDS             complement(3831..>4416)
                     /locus_tag="PLQ54_04065"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOS92462.1"
                     /translation="LLALTGDSPSMGPFGDIAKRATDLRSSVELLRLVASLRDGELLN
                     GDALGDPPDFCAGCVTRGGPPQLRWLQRKVAAGAEFVFTQPIFAIEALRALATDLAGL
                     PLRALIGVLPITSQRMAESLSSGRVPGIDVPPCVLDELARLPGRDAQRRRGLDRAVEL
                     ARVAFHECRGLYLIMPFGPSCYTDAAEIVSAVRD"
BASE COUNT          701 a         1572 c         1515 g          628 t
ORIGIN      
        1 cggtcttgtc agcggcggcc ggggcgtcgg cggagcaggg cgagggctcc cagccatccg
       61 gccgccaggt agatccgata ggcctcgctc gtgtcccggt cgggcagcca cagccgccac
      121 tcacagtcgg gcgcgatggc cacccgcagg aaccagcgcc atgccaccaa cgccgccaca
      181 aggctggccg cggttgtgag gagccatagg tcgtacaccg gccggcacac gccccacgac
      241 agggcgccca tagagccggc gacagtcagg gccaccaggc ttcgcgcccc gacgcgaatc
      301 cgccaatgcc ggtcgtccgc agagccaccc ccggggccgc ccgaagccgc gaacctccgt
      361 atcactgaga gggccaggac tgccgccaaa agcacgcctg tgacgaacag cgcccgcgga
      421 tgcacggcgt cggccgaatc gcgggacgcc tgccactgac tcccgatgga cgccagccgg
      481 tcgtcggggt ggacgagagc cgaggccagg ctccggacgc ccaaccagac ggcggtgccc
      541 aagctcagcc atgactgcgc cgacagcggt gtggccccct ggcggagcaa gctcctctcg
      601 cccgcctgac gaatagccga ctccagctcc agggccaggt ccgaggccag ttccacgttc
      661 tcggagacgg tcgacatgga ctcgaggagc acctggcgag tctgctccgc cgtgctccgg
      721 caccgatcga gattggtgat cgtctcgcgg cgcagacggc ccagcgtgcc cgagccctgt
      781 gaagccgcgt cgtcccatga cggcccggtg actgcccgcg catcggccaa gagccggtcg
      841 tagcgtgcaa gccgcctctg gatggcgaca taggcctgct ggagcgactc gaacacggac
      901 tcgtactgca ggcccctctc cccgagcgcc tcgacgcgca gccgcgcgtc tcgtacgagc
      961 ttggcgccgg cgcgcacgtc gcggggctct cgcctcaggc gggacacgag ctcgcggtgc
     1021 accgcccggg catcggcgag ctcaacctgc cgcgctgcca aggcccgacg gcatgcggcc
     1081 aactccaccc gcgagatctg cgccaggatc ttcagggcgg tggggtccac ctgcgcttgc
     1141 ggcgagggct gcggctccgt gccggacgcg gcggccgccg cggagattga ctcctcgagc
     1201 acccgcaggc gactctcgcc gagtcgctgg ctcatttgct cggcctgggc cgtatcaacg
     1261 gtcgccttgg gtgagtcagc gggcggaggc cgctcgggtg cggtctccgc ctccgtggcg
     1321 ccctctgtct cggccggccc gcccatgggt ggtggctcgg aggcgtccgc tctagtgccg
     1381 gcttccccag acgtcgatga ctccggcttc atttgcggcg gcgcctcccg ccggtcgcca
     1441 gggctagccg gtggcgtctc gtcctctgtc tccggagcgg gagtcacggg aggggcgacg
     1501 tcgccctggg tctcggtctc gcgatggccg ggaaccggcg ccgggcttcg ctcatcctcg
     1561 ccagggccac caggtacctc ctcctgtgcc ggcggaggcg atccactgcc atacgacgcc
     1621 ggtgggccac tgggcgcctg gatcgccgcc gcgaccagcg gcgccacggc gacgccaggc
     1681 gggagaggcg cagcgcgcgg caggccgtgt gccagcggga cggccaccac gatgcccaac
     1741 gcaacagccg acaaggcggc cagacgccgc gcgccgcccc aaccgcgtgg ggcggccgac
     1801 cggtcgtcag aagtagtacc agatcgaccc atactcggcg ggatcctccc cgatgctctc
     1861 aagccgcgcc aggtagtcga gaaccggtac gtcaacgctg acatagggct ggctgggggc
     1921 aatgcccttt tcccacgggt ggaattcgta gacccgcgag ccgtttggga gcaccgcgag
     1981 caagtcacgg tgttgtgccg ccctcaggta taccttgccg agcgccggga tgttgacaac
     2041 cgcttcgtct gtccgccgcg ttcccgggag cagacgcgcc tcctccttct gctcctggag
     2101 tatgcgcgca atgggcactc ggtagtccag cgtctcggcc ttgcccttgg gggcgaaggt
     2161 atagtacgga tcgactccga cccgccgcag cagcatgcgg aggcgcgccg tctcgaaccg
     2221 ccgtgaagca tagaacgtgt agacgacctg gttgtagatg ccgatccctc gcgaccgcag
     2281 gcgggcgatg gcggcgacca tttcgggcgt gacctcgtag gggtgctgaa cgtgcgtgac
     2341 tagtaagatc tcgcggtgtc cgggggagcg aaggctgccc aacaggtcgg ccagggaatg
     2401 ggtgatccgc atggggagcg tcacggggat tcgcgtgccg atccggacga cgttgagcga
     2461 cgcaatcccg cgaacgcgtc ggagtatcct ggccagcgcg tcgtcgggca tggcgagggg
     2521 gtccccaccc gtgatgagca gctcctggat ggccgggtgg gccgcgatcc agtcgcaggc
     2581 ggcctcgatc tgctcccagg gcgccagggc ttcgcaggag ccgggcccgg tgacctccca
     2641 gttccgctgg cagtagacac agaactgcga gcaactgcgg tatggcttga ggatggccac
     2701 ggccgggtag cgccgagtta ccagcgggat gggagaggtc tctccctccc gcatgaagtc
     2761 catggcgccc ggcccgccgt cgcgcatgtg ctctccccac gccgacgcgc actgacagga
     2821 gggcaggacc tgcgccctga gagcccggtc tctgcccgag tctgctacct cgtcaaacag
     2881 cgaggcatag tagggggtca ccgcgaatgg cataccctcc gctgtggccc ggcgaacgcc
     2941 ctcacgctcg tcggcccgca gcgcgacggc ccgatccaga ctgtcgagtc ccgcgatcac
     3001 gtgcgctgcg tgccactgcc agtcggccca gtcagcctca tcgcccccca gggccgccag
     3061 gacctccgct cgccgcaccg acctccgcgc gacggcctcc ggagacaggc cgtcctcata
     3121 gcgctccatc catgaggctg ccgacgtcca cagcgcatcc agcgccacgc tccgctgcgc
     3181 cgcactgcca gggtcctcgc cgctcggatc ctcggccagc ggccccaggc acgcctttcc
     3241 ctgcaggccc tccaacaggt gaaccatctc ggcgtggaac cccggctgaa ggtcgggccg
     3301 atcctgtcga gcggcgacgt cgagaagggc cgagaagacg ctgaagcccg agagtgcttc
     3361 ggagcgctcg gagatcatgc tgcacagagc gcgtccgcag tccctgaccg tgtgtccgag
     3421 ctcggtgtgc ggcacggcct cataggtgtg ccactggcac cgcgagatga cgtcgtacag
     3481 gagtggacgt gctgaggggg atccgtgaag cgaggcgata gcgggcagaa ggggtcccgc
     3541 tgccgatcga acgtgctccg ccgtgtacgc ggcatttcgc atcggtgtct cctttcgagg
     3601 catcgacgcc gcaccggtgc gaccgcgcgg gccggtcgcc cggccatcag gggctccggc
     3661 gcctccgcgc gatggcgcga cgcacaccac ctccgcccca gtgcgcgccg ccccgacggc
     3721 tcgccgggcg gtaccgcact gagcagcata tgtgccacgg ccctacggcc gcggggtcta
     3781 ctatggaccc attcgccgac acagcgccga gtcctctgca cggccctccc tcagtcccgt
     3841 acggcggaga cgatctcggc cgcatcggtg tagcacgagg gcccgaaggg catgatgagg
     3901 tacagccctc ggcactcgtg gaaggccact cgcgccagtt ccacagcccg atcgagtccg
     3961 cgccgtcgct gcgcgtcgcg accaggcagt cgcgcgagct cgtccagcac gcacggtggc
     4021 acatcgatac cgggcacacg gcctgacgag agagactcgg ccattcgttg gctcgtgatg
     4081 ggtagaacgc cgatcagggc gcgaaggggc agtccggcca ggtccgtagc gagggcgcgg
     4141 agcgcctcga tcgcgaagat aggctgcgtg aacacgaact cagcccccgc cgcgaccttc
     4201 cgctggagcc agcgcagctg tggcgggcca ccgcgggtga cgcagcccgc acagaagtcg
     4261 ggtggatcgc ccaaggcatc gccgttgagc agctcgccat cgcgtagtga ggccaccaag
     4321 cgcaagagtt cgaccgagct acgcagatcg gtggcacgtt tggcgatatc gccaaacggc
     4381 cccatggagg gcgagtcgcc tgtcaacgcc agtagg
//
LOCUS       DAOSPL010000154         4409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_19876_length_4409_cov_3.269637, whole genome shotgun sequence.
ACCESSION   DAOSPL010000154 DAOSPL010000000
VERSION     DAOSPL010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19876_length_4409_cov_3.269637"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PLQ54_04070"
     CDS             complement(<1..709)
                     /locus_tag="PLQ54_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92463.1"
                     /translation="MARVGAWIAALTAVGTAQCPAGAVTVQSNAYVARIDAGGLTSLV
                     DASGHAFVTPAGDARSVGILRTAREHWAAVDAEGPDGRMTAFEGLPGTWVEASSTVDD
                     VSGDLVLTQRCSSPEGGVYGLAWVTGNIPLNMSIIVPGNSGVRLDGRAPIDSMSFDYP
                     MTWEAQLVIVEGEGRGFYVWGDDTLGVFKRVTVQRTPDGWRLQFVTMATAPFEPVLGL
                     VSVPWHVNVYEGDWRVPA"
     gene            complement(725..1813)
                     /locus_tag="PLQ54_04075"
     CDS             complement(725..1813)
                     /locus_tag="PLQ54_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS92464.1"
                     /translation="MTGRDIALGTLHREAVPFPCINYCWMTHTRYMSHVAGREYWDDP
                     EGVFIAYVRRSGANLVPQWYYPDEGHRRLEAGEVMHAIDPHVRRGFREPEDVLRYIER
                     LPSDEAVEREFNVEVVAEAYGGNIRQRMEQLGPDVLVIDGFGQADFMGGYSQWGYENY
                     LAASALWPEAMHHYYHHTAVQGRLMNHAIALACERYGLAPFVYGGQDICTAKGPIMSP
                     AALREIYFPELKWCLEPLVEAGIGIVWHCDGDIRPILGDILDLGVIGLQGFEEEHGVD
                     YGEMARLRDAKGRPIAIWGCVSVTSTLPHGTADDVRRAVERSFTIAGPGRGFVLSSTS
                     SVMPEVPLENVDALFTHGRQFGREYLGG"
     gene            1960..2997
                     /locus_tag="PLQ54_04080"
     CDS             1960..2997
                     /locus_tag="PLQ54_04080"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOS92465.1"
                     /translation="MRAIGIHEYGGLDRVQLIEVDEPTAADGEVVIDVRSAALNHLDV
                     WVRNGRPGPGLPMPHILGSDAAGVITAVGEGVPRSRLGEEVVITPGLSCRRCEACIRG
                     EHSECESFGIIGANRPGTFAERVAVPAYCAHPKPRALSFDQAAALPVAYLTSWRMLLS
                     RAKLRPGESVLVHGVGGGVALAALQIAKLIGALVIVTSSSEEKLAKARALGADHSIDY
                     GASPDVAGAVRELTDGRGVDVVVDTVGAATWPIDLSVVRKGGRVVLCGVTTGAEAPTD
                     LRTVYWRQLSLLGSTFGNDEEFRLLLNATESAGLRPVIDSTYDIEDAADAMERMEAGK
                     QFGKLVLRVPH"
     gene            3120..4169
                     /locus_tag="PLQ54_04085"
     CDS             3120..4169
                     /locus_tag="PLQ54_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOS92466.1"
                     /translation="MTRRQFVSSALSLPGALTPVATPNRCERRFRFFVWNDMHVRAQG
                     VAGRPPGYPCAEEKARWALECALGLHDIEPPDFVVSAGDIIDGELEDFDQDFRHLRTA
                     ILDRLPVPFLPCLGNHENAQGEGIEGRTDAYDCCFGPGRRNYAFACGGVAFLVLDTSG
                     AHRQPDDVTRARNALARRALERIGNMPVFLLTHVPLIPMRDEAVLKESFGFSSYKVLD
                     AGLLDVVEAHSEAVVAVLTGHLHLTAAVTRRGVAHITSGGTCGYPADFVSLDVFDDRV
                     DVAMHRAPERWLDRGGDIHGFPRYDHDYTDASHLGHEEYVWGNPDERTLSIRLERAKR
                     PRGTGPAGLQALRVE"
BASE COUNT          715 a         1518 c         1459 g          717 t
ORIGIN      
        1 gagccggcac gcgccagtca ccctcgtaca cgttgacgtg ccaaggcact gagaccaagc
       61 ccaacaccgg ctcgaatggc gcggtagcca tggtgacgaa ctgcaggcgc cacccgtcag
      121 gcgtcctctg taccgtcacc cgcttgaaca cgcccagcgt gtcgtcgccc cagacgtaga
      181 agccgcggcc ttccccctcc acgatgacca gctgcgcttc ccacgtcatc gggtagtcga
      241 agctcatgga gtcgatgggc gcccgtccgt ccagtcgcac cccggagttc cccggaacga
      301 tgatgctcat gttgagcggg atgttgcccg tcacccaggc caagccgtac acgccccctt
      361 cgggagacga gcagcgctgg gtcagcacga ggtcgcccga gacgtcatcg acggttgagg
      421 aggcctctac ccacgttccc ggcaggccct caaatgccgt catgcgcccg tctggccctt
      481 ctgcatccac ggcggcccag tgttcgcgcg cggtgcggag gatgcccaca ctccgggcat
      541 cgcccgctgg cgtcacaaac gcgtgacccg aggcatcgac cagagacgtc agtccgcccg
      601 cgtcgatccg cgccacgtag gcattgctct gaacggtcac cgcgccggca gggcactgcg
      661 ccgtgcccac agcggtcagt gccgcgatcc acgcgccgac tcgagccatg agaaccgcct
      721 ctccctaccc gccgaggtac tcccggccga actggcggcc gtgcgtgaac agcgcatcga
      781 cgttctcgag cggtacctcg ggcatgaccg agctggtcga cgacagcacg aagccccgcc
      841 cgggcccggc gatggtgaag gaacgctcga ccgcgcgccg tacatcgtcg gccgtgccgt
      901 gcggcagcgt cgaggtcacc gatacacagc cccagatagc gatggggcgg cccttcgcgt
      961 cgcggagccg ggccatctcg ccataatcga cgccgtgctc ctcctcgaag ccctgcaggc
     1021 cgatcacccc gaggtcgagg atgtcgccga ggatcggtct gatgtcgccg tcgcagtgcc
     1081 atacgatgcc gatgccggcc tcgaccaggg gctccaggca ccatttcagc tccgggaagt
     1141 agatctctct cagcgccgcc ggggacatga tggggccctt ggccgtgcag atgtcctgcc
     1201 cgccgtagac gaaaggggcg aggccgtacc gctcgcacgc cagagcgatg gcgtggttca
     1261 tgagccgccc ctggacggcc gtatggtggt agtagtggtg catcgcctcg ggccacagcg
     1321 cggacgcggc gaggtagttc tcgtaacccc attggctgta gccgcccatg aagtcggctt
     1381 ggccgaagcc atcgatgacc aacacgtctg ggccgagctg ctccattcgc tggcggatgt
     1441 tgccgccgta ggcctcggcg accacctcga cgttgaactc gcgctccacg gcctcgtcgc
     1501 tcggcaaccg ctcgatgtac ctcagcacgt cctcgggctc tcgaaagccg cggcggacgt
     1561 gcgggtcgat ggcgtgcatc acctcgcccg cctccagccg ccggtgtcct tcgtccgggt
     1621 agtaccactg cggtaccagg ttggcaccgg accggcggac atacgctatg aacactccct
     1681 cggggtcatc ccagtactcg cgccccgcga cgtggctcat gtagcgcgtg tgggtcatcc
     1741 agcagtagtt gatgcacggg aatggaacgg cttcgcggtg gagcgtgccc agtgcaatgt
     1801 cccgtccggt catccagatc cacccatccc ggcagcgttc cggcgacgaa accagggttc
     1861 gggccgtcgg gcctcggacc tgcgcccgga accccgtcgg gtccagtgcg ttgtgcctga
     1921 agggatcgcc cgcaaccaag agagaggacg gggaccgcga tgagggccat cgggatccac
     1981 gagtacggcg gtctggaccg cgtccagctc atcgaggtcg atgagcccac cgcagcggat
     2041 ggagaggtgg tgatcgacgt gcgctcggcg gctctcaatc acctggacgt gtgggtccgc
     2101 aacggccgcc cgggtccagg gctgccgatg ccccatatac tgggctcgga cgccgcgggc
     2161 gtgatcactg ccgtgggtga gggagtgccc cgatcacggc tcggcgagga ggtcgtgatc
     2221 acgccgggcc tctcgtgccg ccggtgtgag gcctgcatcc gtggcgagca cagcgagtgt
     2281 gagagcttcg gcatcatcgg cgccaatcgc cccggaacct ttgccgagcg cgtcgcggtg
     2341 ccggcctatt gcgctcatcc aaagccaagg gctctgagct tcgaccaggc tgccgccctt
     2401 cccgtggcct atctcacgtc ctggcgtatg ctcctgagcc gcgccaagct gcgccctggt
     2461 gagtcagtac ttgtacatgg cgttggcggc ggcgtcgcgc tggccgcgct ccagattgcc
     2521 aagctcatcg gtgcccttgt gattgtaact tcgtcgtcgg aggagaagct cgccaaggcg
     2581 cgagcgctgg gggccgacca ctccatcgat tacggcgcca gccccgacgt ggccggggcc
     2641 gtccgcgaac tgaccgatgg ccgcggggtc gacgtcgtcg tggacacggt tggcgcggcc
     2701 acgtggccga tcgacctcag cgtcgtgcga aagggcggcc gtgttgtgct gtgcggtgtc
     2761 acgacgggag ccgaggcccc taccgacctg cgtaccgtct actggcgcca actcagccta
     2821 ttgggctcca ccttcgggaa cgacgaggag ttccgacttc tgctgaacgc gaccgagtcc
     2881 gcaggactcc ggcccgttat cgactcgacc tacgacattg aggacgctgc ggacgccatg
     2941 gagcgcatgg aggccggcaa gcagttcggc aagctcgtgc tgcgcgtgcc tcactaggcg
     3001 ggcagtgccc tcgtagccgc cgcggcagga acgacctcgc ttcgtgcagg tcatccgggc
     3061 ttcggcaccg caatgccatc ggtgtgagag cagcccgcgc cgctctgagg tgagacgtcg
     3121 tgacgcgaag gcagttcgtc tcttcagccc tcagcttgcc cggtgcgctg acgcctgtgg
     3181 ccacgccgaa tcgatgcgag cgccggttcc gcttcttcgt ctggaatgac atgcacgtac
     3241 gggcccaggg tgtcgctgga cggccgcccg gctacccctg tgccgaggag aaggcacggt
     3301 gggcgctcga gtgtgctctc ggcctgcatg acatcgagcc gcctgacttc gtggtctcgg
     3361 ccggcgacat catcgatggc gagctcgagg acttcgatca ggacttccgg cacctccgga
     3421 cggccatcct cgaccgccta cccgtgccgt tcctcccgtg ccttggcaat cacgagaacg
     3481 cgcagggcga ggggatcgag ggcagaacgg atgcctatga ctgctgcttt ggtcctggcc
     3541 gccggaacta cgcgttcgcg tgcgggggcg tcgccttcct cgtgctggac accagtggcg
     3601 cgcatcggca accggacgac gtgacgcgag cacgcaacgc actggcgcgc cgagcgctgg
     3661 agcgcatcgg caacatgccg gtcttcctgc taacccacgt tccgctcatc cccatgcgtg
     3721 atgaggctgt cctgaaggag agcttcggct tcagcagcta caaggtgctc gatgccggcc
     3781 ttctggatgt ggtggaggcc cactccgagg ccgtggtggc cgtgctgacc gggcatctcc
     3841 atctgactgc ggcggtgacg cggcgtggag tcgctcacat cacttcgggg ggcacctgcg
     3901 gctatcccgc cgacttcgtg tccctcgacg tgttcgacga ccgcgtcgac gtcgcgatgc
     3961 accgggcgcc ggagagatgg ctcgatcgtg gcggcgacat ccacggcttc ccgcgctacg
     4021 accacgacta cacggacgcg agccacctgg gccacgagga atacgtgtgg gggaacccgg
     4081 atgagcgtac attgtcgatc cggctggaga gggcgaagcg gccccgtggc accgggcccg
     4141 ccggcctgca agccctgcgg gtcgagtgaa cgcggaccac aggcgtctcg gggcgaactg
     4201 gaccggaaac acgatcggag tgtggccggc tcgggccata ccaccccgca agagacctcg
     4261 aggcggctgc tcgaacggcg tcggggggca tcctcgccgc cgagtcacct gcccaaccag
     4321 acccgagcca gcccacccca gtggcacgcg cccccaactg gaagcacatg cccagctatc
     4381 cgggcggtgg gggcaccaat ccacacatt
//
LOCUS       DAOSPL010000155         4397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_19936_length_4397_cov_3.579226, whole genome shotgun sequence.
ACCESSION   DAOSPL010000155 DAOSPL010000000
VERSION     DAOSPL010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19936_length_4397_cov_3.579226"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PLQ54_04090"
     CDS             complement(<1..498)
                     /locus_tag="PLQ54_04090"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOS92467.1"
                     /translation="MRWARISVLVPPDATEAVWPVFEAIGCAGIEISETNGATAVSGY
                     LPVCDGMDDLVARVEQGAFDAGVLCGHARKPEVVLTFVEDEDWATSWKAHFHPTRVGR
                     RFLICPSWELTDPQPGGLRIVMDPGMAFGSGTHESTQLALEALEDTVHPGDTVIDVGC
                     GSGILA"
     gene            complement(503..1627)
                     /gene="dnaJ"
                     /locus_tag="PLQ54_04095"
     CDS             complement(503..1627)
                     /gene="dnaJ"
                     /locus_tag="PLQ54_04095"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOS92468.1"
                     /translation="MATEKRDYYEVLGVDRAATKEQVKRAYRQLARRYHPDVNPDDPQ
                     AEERFKELAEAYSVLSDDQKRAHYDRFGHDGPPAMGMGDFGFGIEDLIQSFFGGGFGA
                     RSRQVQRGADLRADVRVTLEEVLTGCEKELSSRKLDYCDDCDGSGSRGGQDLGPCRTC
                     GGAGQVGRTQNTLFGTFSSFSTCPACQGEGVEVKDPCPTCQGRGRLMQESVLTVNIPP
                     GVDTGTRVRVAGQGEAGARGAPPGDLYLYITVADHDVFYRDGSELGIEVPITFSQAAL
                     GADIEVPTLEGKQKLTVPPGTQSGTRLRIRGQGLPSLRGGGRGDLHVFINVRTPTRLS
                     ARQRELLQELAAEDGDTAPEDAQKKPDSLLARAWQKLVGE"
     gene            complement(1703..2080)
                     /gene="rpoZ"
                     /locus_tag="PLQ54_04100"
     CDS             complement(1703..2080)
                     /gene="rpoZ"
                     /locus_tag="PLQ54_04100"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOS92469.1"
                     /translation="MDDLDLNALEGKVGNKYKLVVAVARRAQQLRDGHRPLVQMKSNN
                     PITVALHEIASKEVEVDERDGLIRLKRGGELPGIFSFDREEPTMDDLDDLDLDDVADL
                     DVHGFAGVGDDEDEDLEEGVGEE"
     gene            complement(2294..3595)
                     /gene="nhaA"
                     /locus_tag="PLQ54_04105"
     CDS             complement(2294..3595)
                     /gene="nhaA"
                     /locus_tag="PLQ54_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HOS92470.1"
                     /translation="MLTPLQDFLRHEAAGGMVLMVCAAVALLCANSPWGDRYAALWQT
                     KVGVDVAGKGLSKPLLLWINDGLMAVFFFVAGLEIKREIMVGELSSVRRALVPIAAAA
                     GGMLLPAAIYALLNRGGPGARGWGIPMATDIAFAVGVLPFAGRGLPVAASVFLTSVAI
                     ADDIGATLVIALFYAGGVSGAALAAAGALFAAMCICNWAGVRAIVVYSLLGAGLWVAF
                     LKSGLHPTASGVLAALAIPARSRINADEFVEVGRSWVERFASMGEHGQSILANPEQAA
                     TVVALEETCHHAQTPLQRLEHLVHPWAAFAIIPVFALANAGVSFREAGQGGLVNATSM
                     GIVGGLLVGKVSGVAGLAWLLVRAGLAEMPEGLTWRHIIGLGFLAGVGFTMSLFIAQL
                     AFGESALLAPSKIGILVGSVLAGVIGVTVLRTARSQREWAS"
     gene            complement(3710..3949)
                     /locus_tag="PLQ54_04110"
     CDS             complement(3710..3949)
                     /locus_tag="PLQ54_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92471.1"
                     /translation="MADGTAKPAEPALASPMSLCALVGFGFGDMLGSGVCARVGKAAG
                     LMGSAVWFAVVVSRAAALLTALSCALPGPRFWPRA"
BASE COUNT          763 a         1620 c         1361 g          653 t
ORIGIN      
        1 ggccaaaatg ccactcccgc aaccgacatc gatgaccgtg tcccccgggt gcaccgtatc
       61 ctcgagggcc tccaatgcca gctgcgtgga ctcgtgggtc ccgctaccga aggccatccc
      121 gggatccatg acaatccgca gtccccccgg ctgcgggtcg gtcaactccc acgaggggca
      181 gatcaggaag cggcggccaa cacgggtcgg atggaaatgg gccttccacg acgtggccca
      241 gtcctcgtcc tcgacaaagg tgaggacaac ctcgggctta cgggcgtgac cgcacaacac
      301 acccgcgtcg aacgcgccct gctcgacgcg ggccaccagg tcatccatgc catcgcacac
      361 gggcagatac ccagacaccg cggtcgcacc gttggtctcg gagatctcga taccggcgca
      421 tcctatggcc tcgaacaccg gccacacggc ctcggtcgca tcgggaggaa ccaacacact
      481 gatgcgggcc caccgcactc gactactccc ccacgagctt ctgccaagcc cgggccagga
      541 ggctgtcggg cttcttctgt gcgtcttcgg gcgcggtgtc gccgtcttcc gcggccaact
      601 cctgcaacag ctcgcgctgc cgggcggaca gacgggtggg tgtgcgaaca ttgatgaaca
      661 cgtgcaggtc gccccggccg ccgcctcgga gcgaggggag cccctgacct cggatcctga
      721 gccgcgtgcc cgactgcgtg cctggaggca ccgtcagctt ctgcttgccc tcgagcgtcg
      781 gcacctcgat gtcagcgccc agcgctgcct gtgagaaggt gatgggtacc tcaatgccca
      841 gctctgagcc atcgcggtag aacacgtcgt gatcggccac agtgatgtag aggtacaagt
      901 cgcccggtgg cgctccccga gcacccgcct cgccttggcc cgcgacgcgg acccgagtgc
      961 ccgtgtcgac acccggcggg atgttgaccg tgagcacact ctcctgcatc agccggccac
     1021 gaccctggca cgtggggcac gggtccttaa cttcgacgcc ttcgccctga cacgcggggc
     1081 atgtcgagaa gctcgagaaa gtgccaaaca gcgtgttctg cgttcgaccg acctgcccgg
     1141 caccgccaca ggtccggcac gggcccagat cctgcccccc ccgcgagccc gacccgtcac
     1201 agtcgtcgca gtagtcgagc ttgcggctgc tgagctcctt ctcgcacccc gtgagcacct
     1261 cttcgagcgt cacccgcacg tcggcccgca gatcggctcc tcgctgcact tggcggctcc
     1321 gggcaccgaa cccgccgccg aagaaggact ggatcaggtc ctcgatgccg aagccaaagt
     1381 cgcccatacc catggccggc ggaccatcgt ggccaaagcg gtcgtagtga gcgcgcttct
     1441 ggtcatcgct caggaccgag taagcctcgg caagttcctt gaagcgttcc tcggcctgag
     1501 gatcatccgg gttcacgtcg gggtggtacc ggcgggccag ctgccgatag gcccgcttca
     1561 cttgctcctt ggtggcggcg cggtcgacgc ccagcacttc gtagtagtcg cgtttctcgg
     1621 tggccacggt cggtcctcgg tgctctagcg gttgccgttc gcgctcggac ccggggcgag
     1681 tccagccccg gcctccagga acctactcct ccccgactcc ctcttcgagg tcctcgtcct
     1741 cgtcatcgcc cacacccgcg aagccatgca cgtccagatc ggccacatcg tcgagatcca
     1801 ggtcgtccag gtcgtccatg gtcggctcct cgcggtcgaa agagaagata ccgggcaact
     1861 cgccgccgcg cttgaggcgg atcaggccgt cgcgctcgtc gacctcgacc tctttgctgg
     1921 cgatctcgtg aagcgccacc gtgatggggt tgttcgactt catctggacc aagggccggt
     1981 ggccgtctct tagctgttgg gcccgccgcg ccacggcgac aaccagcttg tacttgttcc
     2041 ctaccttgcc ctcgagtgcg ttcagatcaa gatcatccat cgggagtgca cctccgccac
     2101 ggcgcttgct tgggggcatc acgcgagccc cgaatcccac ggggaccgag gccagtgggc
     2161 tggcagccgt gccagccggt tctcgaccag tgtgccagaa cttcaagttt accggatccg
     2221 cctatgcccg tcaaaaaaga catgtctgaa aaacgaaata cttcttggtg cagccagcgc
     2281 gcttggcagt gtatcaactc gcccattccc gctgggaccg cgcggtccgg agcaccgtca
     2341 ccccgatgac acccgccagc accgagccga ccaagatgcc aatcttcgac ggagcaagca
     2401 gggccgactc gccaaatgcc agttgggcga tgaacagcga catcgtgaag ccgacacccg
     2461 ccaggaaacc gagaccgatg atgtggcgcc acgtcagccc ctcaggcatc tccgcgaggc
     2521 cagcacgcac cagcagccat gcgagtccgg ccacaccaga gaccttgccc accagcaggc
     2581 cgcccacgat ccccatggat gtggcgttca ccaggccccc ttggcctgct tcgcggaacg
     2641 acacacccgc gttggccagc gcgaacaccg gtatgatggc gaaggctgcc cacgggtgca
     2701 ccaagtgctc gagccgctga aggggagtct gggcatgatg gcatgtctcc tccagcgcga
     2761 ccacggtggc ggcctgttcg gggttcgcca ggatcgactg accgtgttcc cccatggacg
     2821 cgaaccgctc gacccacgac cggccgacct cgacgaactc gtcggcgttg atccgcgacc
     2881 gggccggaat ggccaacgcg gcgagcacgc cggaggccgt cgggtggagg ccggacttga
     2941 gaaacgcgac ccacagcccg gcgcccagca agctgtagac cacgatggcg cgaaccccgg
     3001 cccagttgca gatgcacatc gcggcgaaca gggcgcccgc ggcggccaga gcggccccgg
     3061 agacaccgcc ggcgtaaaac agggcgatca ccaacgtcgc gccgatgtcg tccgcgatgg
     3121 ccacggacgt gaggaacacg ctggcggcca ccggcaggcc gcggccggcg aagggcagca
     3181 cgcccacggc gaacgcgata tcggtggcca tggggatgcc ccagccgcgg gcccccggcc
     3241 ccccgcggtt cagtagcgcg tagatggcgg ccggcagcag catacccccg gccgccgccg
     3301 cgatgggcac gagagcacgg cgtactgacg acagctcgcc caccataatc tcgcgcttga
     3361 tctcgaggcc ggccacgaag aaaaacaccg ccatgaggcc gtcgttgatc cacaacagca
     3421 ggggtttgct cagccctttg ccggcgacgt cgacacccac cttcgtctgc cacagcgccg
     3481 cgtagcgatc accccacggg ctgttcgcgc agagcagcgc caccgcagcg cacaccatga
     3541 gaaccatccc gccagccgcc tcgtggcgca gaaagtcctg aagaggcgtc agcaagcgat
     3601 cgacgggctc ccgtcggctc gaagagcggc tgtgacccat gctctctccc ctgcgggcgt
     3661 ctgcgttcgc cctgcggctc tggctcgtcg gccaacgtcg ccatcgatgt caggcccgag
     3721 gccagaatcg cgggcccggc aaggcacatg agagtgcagt cagcagcgca gctgccctac
     3781 tgaccacgac ggcgaaccac acggcgctgc ccatgagccc cgctgctttg ccgacccggg
     3841 cgcagacccc cgacccgagc atgtcgccga agccgaagcc caccagggcg cacaggctca
     3901 tcgggctagc cagcgccggc tccgcaggct tcgccgtgcc gtctgccatc cactcacctc
     3961 accgccggca accagctcta gtcccggcgg ccaggattcc ggccacgctg cgtgatccct
     4021 cgtcagtccg tcgggccacc acccaaccga acgctcgctc gcaccgacgc cgcccccgcg
     4081 gcttccgccg cgctctttgg ggccgacgtc tgggatgccc gccggcctgt ccgtgacgac
     4141 atggccaagg ccgcgaagcg gcagggcgag cccttggcct ccgagaccgc gcaagccatc
     4201 gagcgggccg gggctcggcg cccttcgggc gcactcctgt gcggcccgtc cacgacatgg
     4261 gctcaccggg ttgcggtggg cccccaacgc gcccggtctc caggccacca caccgggccg
     4321 gatagggtcc caccgggctc cgcccctatt cggcccctcc gaacacctcg cgctggcaga
     4381 ggaggggccg aatggaa
//
LOCUS       DAOSPL010000156         4391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_19978_length_4391_cov_3.301891, whole genome shotgun sequence.
ACCESSION   DAOSPL010000156 DAOSPL010000000
VERSION     DAOSPL010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19978_length_4391_cov_3.301891"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2315)
                     /locus_tag="PLQ54_04115"
     CDS             complement(<1..2315)
                     /locus_tag="PLQ54_04115"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOS92472.1"
                     /translation="MRTACLVIAGLACAAVAAAIDVPNAGMEVGAEGPEGWTWAPGEG
                     GQGHFAVDTEHAHSGARSFRVTRVGAAGYLDLTSPRLAVEPGKTYEVAAWVYPLSRVK
                     RGVYFMVNQFAQADGALTLPNTFGFTGQTLAVGEWQRLTVRVTIREGMDHIAIHCIHA
                     MAPSDVCWDDFTVTEAGELPPPRYERPAPEALPDLAPAVETVRQRERARAAIEIRQGR
                     PRLVIDGQTVPWNFYVGPVWDTNQCQIADFRDANIRVYCVPLVMGRNVWGDRGPWRGP
                     GQFDFAEVDDLLWRVLRVDPEGYIIFYMGCDPYASWGAEHLDDVTQDQHGQHAIVDMH
                     PKRWGEDPGPGERFGPSPVSPTLRADMADAMRRLVAHVESSEPGKAVIGYHVAGFNDG
                     QWFQWERLVEGDLHLSDYCPGAVASFREWLRRRYNHDEEALRRAWARPDASFDTVTVP
                     SEARRMGPGFFLDLPQDQDIADHTRFYSEGVAETVEMLAGVLKAETKRPIICGTYYED
                     ITCNSANHIALGRHLDSPAMDYLTGPAAYGIRMAGHQGAVRNVHGSTLLHGKMYLTEQ
                     DWRSWHSEPNEPEQNFDWGRAEDAETHNAMVRRESGMMLAFGLGTWWYDMGGGWFHDP
                     AIMSAVAEARRAFDRDLAVGPNRADLAVVVSEESDAVVRPGAGGFARFRGVLEQIHEI
                     NTAGVPYKVYLLPDLARDDIPKHRAYLFLNAYQLTERDRAAIDQLKGEGRLLVFLHAP
                     GYAGAADPAATISELTGIGVRRLPEDARLSL"
     gene            2431..2964
                     /locus_tag="PLQ54_04120"
     CDS             2431..2964
                     /locus_tag="PLQ54_04120"
                     /inference="COORDINATES: protein motif:HMM:NF038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Os1348 family NHLP clan protein"
                     /protein_id="HOS92473.1"
                     /translation="MRIWIQRKARDVRHALQEIRVGDGVGWSTRSVRTTIVGGRPPGD
                     DARVEVPIGIERVLRRAAADGAFRARLWTDRDGALRASGLALTPAERAILLAAPAEQL
                     EAMVAQLAPTSPSRRSWLRRLVAAAGIVIFGAVTQPGCAVTVATGAQPDLPEGVEAAD
                     ASAPPKTRGITADSSDP"
     gene            3060..3656
                     /locus_tag="PLQ54_04125"
     CDS             3060..3656
                     /locus_tag="PLQ54_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92474.1"
                     /translation="MPGWLGRKSRDLRHALQELKAGDAIDRLRNGVRTTIVGGRPPGD
                     DAVVDVPMGIERVLRKAAADGRFRTRLMSDRAGALERSGLELSVSEHAILLAIPAKHL
                     QAMIAQLAPSSPARRGLLRRLAAAAGIALFGVAGATSQMGCRKRETTTLGITPDFPAT
                     MGISPDMSRLQGIRPDDPLRGSGKRGPNAPPADRPAGE"
     gene            3663..4109
                     /locus_tag="PLQ54_04130"
     CDS             3663..4109
                     /locus_tag="PLQ54_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92475.1"
                     /translation="MPPCPLARVLVGVGNHACDGYASREVPLRQEAFAKCLMCGERIP
                     LHTRWCPHCGRELLDRHVWYNKHASDIVGALLGDALALFWMVQIIQSTERNYRDGPLE
                     LSAEPALLTGVLLGLLVGFLAWDFVRDAMDDGLSTTVGRWQRRYFG"
     gene            4106..>4391
                     /locus_tag="PLQ54_04135"
     CDS             4106..>4391
                     /locus_tag="PLQ54_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92476.1"
                     /translation="MSADITLVNLNMLYMRYYDKVERELHVPLGSLYLTSALEQAGFE
                     VDFRDYQLNDHPEPFSAEAILEFTRDPAPVVGFSCMANLLPFAVLALQKVK"
BASE COUNT          676 a         1539 c         1484 g          692 t
ORIGIN      
        1 tccaaggaga gccgggcatc ctccggcaac cttcgcacgc cgatgcccgt caactccgat
       61 atggtcgcgg ccgggtccgc cgcgccggcg tatcccggag cgtgcaggaa cacgagcagg
      121 cgcccctcac ccttgagctg gtcgatggcc gcgcgatcgc gttcggtgag ctgataggcg
      181 ttcaagaaca ggtaggcgcg gtgcttcggg atgtcgtcgc gtgccaggtc ggggagcagg
      241 tagaccttgt agggcacccc ggccgtgttg atctcgtgga tctgctcgag cacgccacgg
      301 aaccgggcga agccgccggc gccgggcctg acgacggcgt cactttcctc gctgaccact
      361 acggccaagt cggcccggtt cggaccgaca gccagatccc ggtcgaaggc ccgccgcgcc
      421 tcagcgacgg ccgacatgat ggccggatcg tggaaccagc ctccgcccat gtcataccac
      481 caggtgccga ggccgaaggc cagcatcatg cccgattcgc ggcggaccat ggcgttgtgc
      541 gtctcggcgt cctcggcgcg gccccaatcg aagttctgct ccggctcatt cggctcggag
      601 tgccacgatc gccagtcctg ctccgtcagg tacatcttcc cgtgcagcag cgtcgagccg
      661 tgcacgttgc gcaccgcgcc ctggtggccc gccatgcgga tcccatacgc ggccgggccg
      721 gtgaggtagt ccatggccgg gctgtcgagg tgccggccca gggcgatgtg gttggcgctg
      781 ttgcaggtga tgtcctcata gtaggttccg cagatgatgg gccgcttcgt ctcggccttg
      841 agcactcccg ccagcatctc gaccgtttcg gccacgccct cgctgtagaa gcgtgtgtgg
      901 tcggcgatgt cctgatcctg aggcagatcg aggaagaagc cggggcccat gcgccgcgcc
      961 tcgctcggga ccgtcacggt gtcgaaggag gcgtccggac gtgcccacgc ccgccgcagc
     1021 gcctcctcgt cgtggttgta gcgccgccgc agccattcgc ggaagctggc cacggcgccg
     1081 gggcagtaat cggaaaggtg caggtcgccc tcgacaaggc gctcccactg gaaccactgg
     1141 ccgtcgttga agccggccac gtgatagccg atgaccgcct tgcccggctc gctggactcg
     1201 acgtgcgcca ccagtcgccg catggcgtcg gccatgtcgg cccgcagcgt cggcgacacg
     1261 ggcgagggac caaaccgctc gcccggcccc gggtcctcgc cccagcgctt ggggtgcatg
     1321 tcgacaatcg cgtgctggcc gtgttggtcc tgggtcacat cgtccagatg ctccgctccc
     1381 cagctcgcat aggggtcgca gcccatgtag aagatgatgt agccctcagg atcgacgcgc
     1441 agcacacgcc acagcaggtc gtcgacctcg gcgaagtcga actggcccgg tcctcgccac
     1501 gggcctcggt cgccccacac gttgcggccc atcaccaggg ggacgcagta gacgcggatg
     1561 ttggcatcgc ggaagtcggc gatctggcac tggttggtgt cccacaccgg cccgacgtag
     1621 aagttccacg gaacggtctg gccgtcgatc accagccgcg gccggccctg acggatctcg
     1681 atggccgcgc gggcccgctc gcgctggcgc accgtctcca ccgccggcgc caggtcggga
     1741 agcgcctccg gcgccgggcg ctcgtaccgt ggcggcggca gctcgccggc ctcggtcacc
     1801 gtgaagtcgt cccagcacac atccgagggc gccatggcgt ggatgcaatg gatggcgatg
     1861 tgatccatgc cctcgcggat ggtgactcga accgttagtc gctgccactc tccgaccgcg
     1921 agtgtctgcc cggtgaagcc gaaggtgttc gggagggtga gcgcgccgtc agcctgggcg
     1981 aactggttca ccatgaagta gaccccgcgc ttcacgcgcg acagcggata cacccaggcc
     2041 gccacctcat aggtcttgcc cggctcgacg gccagacgag ggctggtgag atcaaggtag
     2101 cccgccgctc cgacccgcgt gacccggaac gaacgggcac ccgagtgggc gtgttcggtg
     2161 tcgacggcga aatggccctg gccgccctcg cccggcgccc aagtccagcc ttccgggccc
     2221 tcggctccaa cctccatacc ggcgttgggc acatcgatgg cggccgccac ggccgcgcag
     2281 gcgaggcccg ctatcacaag gcatgccgta cgcatggcga gactcccacc ctccaggaag
     2341 cggcgctcgt ccccctttcc tcccgcgcga ggaccttcct gcccactggc gaatgctccg
     2401 gcaccattac cttctgcggg ggtgtcggcc atgcggattt ggatccaacg caaagcgcgc
     2461 gatgtgcgcc acgcactcca ggagatccgg gtcggcgacg gagtcggctg gagtacccgc
     2521 agcgtgcgca ccactatcgt gggggggcga ccgcccggcg atgatgcccg ggtcgaggtg
     2581 cccatcggca ttgaacgcgt gctgcgccgt gcggcggctg acggcgcctt ccgcgcgcgg
     2641 ctgtggactg accgcgacgg cgcactgcgc gcgtccggcc tggcgctgac acccgccgag
     2701 cgcgccatct tgttggccgc cccggccgag cagctcgaag ccatggtggc tcagctcgca
     2761 cccaccagcc cgtcgcgacg aagctggttg cgccgactgg tggctgccgc cggcatcgtc
     2821 atcttcggcg ccgtcaccca gccgggctgc gcggtgacgg tcgcgacggg agcgcagccc
     2881 gatctgcctg agggcgtcga ggcagccgac gccagcgcgc cgcccaagac ccgaggcatc
     2941 acggcggact ccagcgaccc ctgacgcagt gctgacccaa cgattcgcgc gcggagagcc
     3001 accgacacac tggtgatagc ctctctcaac cggacgccgt ccccaggagg cggttggaca
     3061 tgccaggctg gctcggacgt aagtcgcgcg acctgcgcca cgcactgcag gagctgaagg
     3121 ctggcgacgc catcgaccgg ctgcgcaacg gcgtgcggac gacgattgtg ggcggacggc
     3181 caccgggcga tgacgccgtc gtcgatgtcc ccatgggcat cgagcgcgtg ctccgcaagg
     3241 ccgcggccga tggccggttc cgcacgcggc tcatgtctga ccgcgccggc gcgctggagc
     3301 gctccggtct cgagctctcg gtctccgagc acgccatcct gttggccata ccggctaagc
     3361 acctgcaggc catgatcgcc cagcttgccc cgagcagccc agcacggcgt ggcttgctcc
     3421 gaaggctggc cgcggccgcg ggcattgccc tcttcggcgt tgccggcgcg accagtcaga
     3481 tggggtgccg gaagagggag accaccacgc tcggcatcac ccctgacttc ccggcaacca
     3541 tgggtatcag ccccgacatg tctcgcttgc agggcatccg gcctgatgac ccgttgcgcg
     3601 gatcgggcaa gcggggcccg aacgcgcctc ccgccgacag gccggccgga gagtaggtca
     3661 tcgtgccgcc gtgtcctctg gcccgcgtgc tcgtaggggt tggcaaccat gcttgcgatg
     3721 gctatgccag ccgcgaggtg ccactgaggc aagaggcgtt cgcgaaatgc ctgatgtgcg
     3781 gggagcgcat cccgttgcac acgcggtggt gtccgcactg tgggcgcgag ctgctggacc
     3841 gccacgtctg gtacaacaag cacgcatcgg acatcgtggg agcgctcctg ggcgatgccc
     3901 tggcgctctt ctggatggtg cagatcatcc agagcaccga gaggaactac cgcgatggcc
     3961 cgctcgaact ctccgcagag ccggctctgc tgaccggcgt gctgctcggg ctgctcgtgg
     4021 ggttcctcgc ctgggacttc gtgcgagatg ccatggacga tggcctgtcg acgacggtgg
     4081 gcagatggca acggaggtac ttcggatgag cgcggacatc acgctggtca acctcaacat
     4141 gctctacatg cgctactacg acaaggtcga gcgagagctg cacgtgccgc tcgggtcgct
     4201 gtacctcacg agcgcgctgg agcaggcggg cttcgaggtc gacttccgcg actaccagct
     4261 caacgaccac ccagagccct tctccgccga ggccattctc gagttcaccc gtgacccggc
     4321 gccggtggtc ggcttcagtt gcatggccaa cctgctcccc ttcgccgtac tggcactgca
     4381 gaaggtcaag g
//
LOCUS       DAOSPL010000157         4382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20037_length_4382_cov_3.055697, whole genome shotgun sequence.
ACCESSION   DAOSPL010000157 DAOSPL010000000
VERSION     DAOSPL010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20037_length_4382_cov_3.055697"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLQ54_04140"
     CDS             complement(<1..421)
                     /locus_tag="PLQ54_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92477.1"
                     /translation="MRSAHHVIIVIALSAAIAGCGSGAPVDQTVEPPSLQVQVTCTEH
                     WLDASGAAGRALLIDTPFNKVQITVDEQPEGGTLTFTPLYIKEGVQVYTPTGNTAGLR
                     QAGTWDTVFTVISPRTHCLPGHYVLTILPYVETSVIDS"
     gene            complement(471..1706)
                     /locus_tag="PLQ54_04145"
     CDS             complement(471..1706)
                     /locus_tag="PLQ54_04145"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOS92478.1"
                     /translation="MLQAKLSPTLLAALSCGGGALLIGASVAGGAETERWQIGTPIAT
                     YWAGPPMTDATAEQMADGGFNVVWCTPDELDTVQRHGLRALLASGLIAPESLDDPAKR
                     QELDALIARVKGHPALYAYFVTDEPNASRFAALGKLVAYLRERDPAHLAYINLFPSYA
                     TNEQLGTAGDHEAAYAEHLRQYVDVVKPDLISYDHYHYTSAGDGGWYFLNLAMIRQKA
                     LDAGLPFLNIVQASSWTPSMRVPNGDELAWLVYTSLAYGAQGISYFVYYYPDWQGAMA
                     NADGTTTPLYDAASHLNREFVAIAKELQPLTSLGAYHLGELPRGAEALPDDSPVKVVG
                     AQPAQGLVVGLFGRDGTASHAVIVNRDYRWGVRVTLSVPRDTRVFGGAAAGWSPTYGN
                     GSVTVELGPGRGKLVRWKR"
     gene            complement(1800..1937)
                     /locus_tag="PLQ54_04150"
     CDS             complement(1800..1937)
                     /locus_tag="PLQ54_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92479.1"
                     /translation="MMLWDWAMIVPRGRPGELSSTMRDMVARGDISPDVARRLNVLSA
                     K"
     gene            complement(2145..>4382)
                     /locus_tag="PLQ54_04155"
     CDS             complement(2145..>4382)
                     /locus_tag="PLQ54_04155"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOS92480.1"
                     /translation="SLARPAERFLARIGLDRNVDNTPASVRFHVAVGGQDVFATDVLR
                     AGAPPQDIDVSLAGATELELIVDVGPDDRGWDQANWANARVILDDGSEVWLDDLARSA
                     ELQAGPPFSFVYDGRPSSELLSTWDRSEATEERADGSIVKTLTFRDPATGLEVEATCT
                     IYTDTAGADWALRFTNGGDADTPVLEQVQAVDATLGLGPGSQVTLHRLNGAPCLVDDW
                     MPFDQPVPPGQAVAFSATNGRSSNVSPFFNVSWGGGGVITAIGWSGQWASSVGLQEDR
                     LRIRAGTENLRTVLHPGESIRSPRILQLYWTGDDSWRAYNLFRRTMLAHIVPREGDGP
                     AFPPIAHLSTSFYELNATNQENVLSHLNSVKGLGFEVFWLDAYWTGPDGFPNSMGNYG
                     LPLERVEPRDRFPDGLAAIGRAVSDVGLKFLMWFEPERVAPGTRIAREHPEWVLSPGG
                     DGSGLFHLGIPEARAYMTEYLDAAIKGYGLSWLRIDYNIDPLTFWQFGDQDRPDRLGM
                     TEMRYIEGLYQMWDDLRAANPGLLIDDCASGGRRVDLETCSRSLILWRSDNTCDMVGS
                     SRDSILMAAVKNQLMSAGLNRYLPMSIVGQMGAAPYFFRSGFNGGIAFGEDVRPADYP
                     RDQLKQAIAECRRIRKYWLGDFYQLSEATPDPRDWCVLQYHRPEQEDGIVLAFRRHLS
                     PYSGYDCALRAIDPNATYAVTVSTTYEPSPEVTMHGAELQRLNIVIHECPGSALLEYR
                     KTGGG"
BASE COUNT          709 a         1417 c         1528 g          728 t
ORIGIN      
        1 ccgagtcgat gacggacgtc tcgacgtacg gcaggatcgt cagcacgtag tggccgggca
       61 ggcagtgtgt tcgcggggag atcaccgtga agacggtgtc ccaggttccg gcctgcctga
      121 gtccggcagt gttcccggtc ggcgtgtaga cttgcacgcc ctccttgata tagagcggcg
      181 tgaaggtgag ggtgcccccc tcgggctgct catcgacagt gatctgcacc ttgttgaacg
      241 gtgtatcgat gagcagtgct ctcccggcgg ctccgctggc atcgagccag tgctcggtgc
      301 aggtcacctg gacctgaaga gacgggggct cgaccgtctg gtccacgggt gcgccgctcc
      361 cacacccggc gatggctgcg gaaagggcta ttactatgat cacgtggtgc gccgaacgca
      421 ttgcgccaac caccattcct tcctgaagcg gcccagtatg tctcgggcct ctatcgcttc
      481 caccgcacga gcttgcctcg gcccggtccc agctcgacgg tgacacttcc gttgccatag
      541 gtcgggctcc atccggccgc cgcgccgccg aacacacggg tgtcccgcgg cacggagagc
      601 gtcacacgca cgccccaccg gtagtcacgg ttgacgatga cggcgtggct ggccgtgccg
      661 tcgcggccga aaaggccaac cacgagcccc tgggcgggct gggcccccac gaccttcacg
      721 ggcgaatcgt cgggtagcgc ctcggcaccg cgcggcagtt cgccgaggtg gtacgcgccg
      781 agagacgtca gcggctggag ctccttcgcg atggccacga actccctgtt caggtgagag
      841 gcggcgtcgt agagcggcgt ggtggtgccg tcggcgttcg ccatggcgcc ctgccagtcg
      901 gggtagtagt agacgaagta ggagatgccc tgggcgccat acgccagcga cgtgtacacc
      961 agccacgcga gctcgtcgcc gttggggacg cgcatggagg gagtccacga ggaggcttgg
     1021 acgatgttga ggaacggcag gccggcgtcg agggctttct gccgaatcat ggccaggttc
     1081 aggaagtacc agccgccatc gcccgccgac gtgtagtggt agtggtcgta gctgatcaag
     1141 tcgggcttga ccacatcgac gtactgccgc aggtgctccg cgtaggccgc ctcgtggtca
     1201 ccagctgtgc cgagctgctc attggtcgcg tagctgggga agaggttgat gtaggccagg
     1261 tgggccgggt ctcgctcgcg caggtacgcc accagcttgc cgagggccgc gaagcgtgag
     1321 gcgttgggct cgtcggtgac gaagtaggcg tacagggccg ggtggccttt gacgcgggcg
     1381 atgagcgcgt cgagctcctg tcgcttggcg gggtcgtcga gggattccgg cgcgatgagg
     1441 ccactcgcca ggagggcgcg caggccgtgc cgttggacgg tgtcgagctc gtcgggagtg
     1501 caccacacga cgttgaaccc gccgtcggcc atctgctctg ccgtggcgtc cgtcatgggc
     1561 ggtccggccc agtaggtcgc gatgggggtc ccgatctgcc atcgttccgt ttcggctccg
     1621 ccggccacag acgcaccgat aaggagtgca ccaccgccgc acgacagagc cgccaggagt
     1681 gtcggcgaca acttagcctg gagcatgccc actcacctct cggtctcgcg cacgtccttg
     1741 gacactggtt gatctaccgc cctgtttcgc ccacgagcca gggactccct tggcgggcgt
     1801 cacttagcag acagcacatt cagccgtcgg gccacatccg ggctgatgtc gccccgggca
     1861 accatgtcgc gcatggtcga actcaactcg cccggccgcc cccgcgggac aatcatggcc
     1921 cagtcccaga gcatcaccga gtcgcgcttc gatgcctaca aggggggatg tccgggcatg
     1981 gaagcgcggg cgtcatgtcg gcgaaggcgc gcagccagcg gagtaggtcc tctccgcaga
     2041 caccacgtgg taccgaccca cgggcgcgcg cgtccacgtg ggcgatcgcg gcgtgccggt
     2101 tgcggcgagg gtcatgagga gcgctcgtgt caggtgagag cgctctaccc tccgccggtc
     2161 ttccggtact ccagcagcgc cgaccctggg cactcgtgga tgacgatgtt aagccgctgc
     2221 agctcggcgc cgtgcatcgt gacctccggg gagggctcgt aggtggtgga gacggtcacc
     2281 gcgtaggtcg cgttcgggtc gatcgcccgc agggcacaat cgtagccgct gtagggtgac
     2341 aagtgccggc ggaacgcgag cacgatgccg tcctcctgct ccgggcggtg gtactgcagc
     2401 acacaccagt cgcgggggtc gggggtggcc tcggagagct ggtagaagtc gcccaaccag
     2461 tacttacgga tccgcctgca ttcggcgatg gcctgcttga gctggtcccg cgggtagtcg
     2521 gcgggccgga cgtcctcgcc gaaggctatg ccgccgttga agccgctgcg gaagaagtac
     2581 ggcgcagccc ccatctggcc cacgatgctc atcggcaggt agcggttcag cccggcgctc
     2641 atgagctggt tcttgacggc ggccatgagt atcgagtcgc ggctcgaacc caccatgtcg
     2701 cacgtgttgt cgctccgcca gaggatcaga gaccgcgagc aggtctcgag gtcgacccta
     2761 cggccgccgg aggcgcagtc gtcgatcaga agacccgggt tcgcggcgcg aaggtcgtcc
     2821 cacatctggt agaggccctc gatgtagcgc atctcggtca tgcctaaccg gtcgggacgg
     2881 tcctggtcgc cgaactgcca gaatgtgaga gggtcgatgt tgtagtcgat ccggagccag
     2941 ctcagcccat agcccttgat ggccgcgtcg aggtactcgg tcatgtacgc ccgcgcttcg
     3001 gggatgccca ggtggaacag cccgctgccg tcgccgcccg gcgagaggac ccactccggg
     3061 tgttcgcggg cgatgcgtgt ccccggcgcc acgcgctcgg gctcgaacca catcaggaac
     3121 ttgagcccca catcgcttac cgctcggccg atggctgcca agccatcggg gaagcggtcc
     3181 ctgggctcga cccgctcgag gggcagaccg tagttgccca tgctgttcgg gaagccatcc
     3241 gggccggtcc agtaggcgtc caaccagaac acctcgaagc cgagaccctt caccgagttg
     3301 aggtgagaga ggacgttctc ctggttcgtc gcattgagct cgtagaagga agtgctcagg
     3361 tgtgcaatgg gagggaaggc cgggccgtcg ccctctcgcg ggacgatgtg cgccaacatg
     3421 gtgcgccgga acaggttgta ggcccgccag gaatcgtcac cggtccagta gagctgcagg
     3481 atccgcggac tgcggatcga ctcgcccggg tgcagcacgg tccgcaggtt ctccgtgccc
     3541 gcccgaatgc gcagccggtc ttcctgcagg ccgactgagg acgcccactg gcccgaccag
     3601 ccgatggccg tgatgacgcc gccgcccccc cagctcacgt tgaagaaggg cgagacgttc
     3661 gacgaccggc cgttggtggc cgagaaggcc acggcctgcc cagggggaac gggctggtcg
     3721 aaaggcatcc agtcgtccac gaggcacggt gccccgttca gccggtgaag ggtcacctgg
     3781 ctgccgggcc cgaggccgag agtcgcatcg accgcctgca cttgctcgag cacgggagtg
     3841 tctgcgtcgc ccccattcgt gaagcgcagg gcccagtcgg cgcccgccgt atcggtgtag
     3901 atggtgcatg tggcctcgac ctcgagccct gtggcggggt cgcggaacgt cagtgtcttg
     3961 acgatactgc cgtccgcccg ctcctcagtc gcctctgacc gatcccatgt gctcaggagc
     4021 tcggacgaag ggcgtccgtc gtagacgaac gagaacggcg gaccggcctg gagctccgcg
     4081 ctccgggcca ggtcgtctag ccagacttcg gagccatcat ccaggattac tcgggcgttg
     4141 gcccagttgg cctggtccca gccacgatcg tcgggcccga cgtccacgat gagctcgagc
     4201 tcagtggcgc cggcgaggga cacgtcaata tcctgcgggg gcgcgcccgc gcgcagaacg
     4261 tccgtcgcga aaacgtcctg tccccccacg gcgacatgaa agcggacgga ggcgggggtg
     4321 ttgtcgacat tacggtcgag gccgatgcgg gcgaggaacc gctcggcagg gcgtgccaga
     4381 ct
//
LOCUS       DAOSPL010000158         4366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20128_length_4366_cov_3.277662, whole genome shotgun sequence.
ACCESSION   DAOSPL010000158 DAOSPL010000000
VERSION     DAOSPL010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20128_length_4366_cov_3.277662"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1825)
                     /locus_tag="PLQ54_04160"
     CDS             complement(<1..1825)
                     /locus_tag="PLQ54_04160"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOS92481.1"
                     /translation="MSVGRLGSRALSVLAALLLSSAALHAGPVAPIRVLLLGGLNTHE
                     AQAALEPGQSTRLVKRILEDTGRFAVDVTEDPSTLRPGTLASYAVVVDNWSNYPAPDC
                     PWGEEAQRALLAFVRAGGGFVAIHASTACFPAWPQYRDLIGIYWEEGKANHARIHTFA
                     VKPAVRDHPVAKGLAEFRITDELYQRLTLGPRAKVLCTAYSTPEAGGTGEIEPMVVAT
                     HYGRGRGFSIVLGHDGVAMDSDGWRLLFARGVEWAATGQSTVADPYDVDVALLPVLGY
                     QCTDRRDRLAVVEGLVRRAAADPGRRALVAAKLAAAIEADATPDARRFLFDQLSRIAT
                     AAEVPTACRWLGDVELGSRAVQVLQRLDDPAAASALRAATLRLRGRPLVGVLTALGAK
                     RDRGAVPLLVRHAASSDREVALAAVDALGQVGGVRAVDVLRRARAEAPADWSAPIAHA
                     LIRALESLDPGDPGALAEWRSIWASAAPMPVRRAAYHLLVRGTDDASLVFDGLRSGDA
                     VTAAIAAEAMAGPGRAPLRRVAAEGLWGFSPAVQVIVLGALARDPDPTLAQQVEPLLD
                     SGDRLVRSAAREALRRCGAAVPASHSAAFTDLIDQVSEDYQI"
     gene            complement(2015..2284)
                     /locus_tag="PLQ54_04165"
     CDS             complement(2015..2284)
                     /locus_tag="PLQ54_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92482.1"
                     /translation="MRRFIAWGVMVLFCGSVLVGCSSKAKEPEKVTAKKSASEQRKGE
                     MFDAVKSTRDAMQKAGQASGVAGSGKMPGAMGGMKNQAAGAGSGE"
     gene            complement(2338..2601)
                     /locus_tag="PLQ54_04170"
     CDS             complement(2338..2601)
                     /locus_tag="PLQ54_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92483.1"
                     /translation="MTMSQQAMANITAPASVMMFIECLNAPATPNGSCAFAGSRVDWI
                     QARHNGGTNLAYADGHVKWQKHELVKNYTGKGSGIYYTDGVDR"
     gene            complement(2635..2970)
                     /locus_tag="PLQ54_04175"
     CDS             complement(2635..2970)
                     /locus_tag="PLQ54_04175"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS92484.1"
                     /translation="MRRRGFTLIELLVVIAIIAILAAILFPVFAKAREKARQTTCASN
                     LKQIALGNLMYANDYDERFMPFCGDGASWCKNPPGCFYTTGYLQPYIKNKQIWLCPST
                     TGHNCAPCC"
     gene            complement(3213..4097)
                     /locus_tag="PLQ54_04180"
     CDS             complement(3213..4097)
                     /locus_tag="PLQ54_04180"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOS92485.1"
                     /translation="MLSRDLRRKLSRIGAIPPPADGAAPQVRPPRPSRPAPWDIPEDS
                     SPVFFPVADEGDEFVDLLGGGEIDADFGPFWLTELEVEGVWPRARALLDDLATRPGAA
                     PDIPEMQGLEPSDLLVFDIETAGLMGATLFLAGVLDFLSGAPRLRFFLARDYDEEPAM
                     LDALAAHMARTRAIVTFNGKSFDVPFLADRMRYHRLRFDPPPVHIDILHAARRRWRGR
                     FENCRLQTLERELCGRHRTDDVPSDLIPAVYHEFVATQDAELLVPILQHNALDLLTLW
                     ELLCATLATPVPNRAENR"
     gene            complement(4143..>4366)
                     /locus_tag="PLQ54_04185"
     CDS             complement(4143..>4366)
                     /locus_tag="PLQ54_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92486.1"
                     /translation="EAIERLDLASVPGRGYALLTQTLVRAQRLGLRIAEVPITFTERR
                     HGHSKMSSAIIGEQLRLLGRLAWQRVRRG"
BASE COUNT          717 a         1495 c         1494 g          660 t
ORIGIN      
        1 cgatctggta atcttcggaa acctggtcga tgaggtcggt gaatgccgca ctgtgcgatg
       61 ctggcacggc cgcgccacaa cggcggagcg cttcccgggc ggcggagcgg accagacggt
      121 cgccggagtc gagcagtggc tccacctgct gggccaacgt cggatcgggg tcacgcgcca
      181 gggcgccgag gacgatgacc tgcacagccg gactgaagcc ccagagtccc tcggccgcca
      241 cccggcgcag cggcgccctg ccaggcccgg ccatcgcctc tgcagcgatc gccgccgtca
      301 ccgcatcccc gctccgcagc ccgtcgaaca ccagcgaggc gtcatccgtc ccacgaacca
      361 gcagatggta cgccgccctg cgaacgggca tcggcgccgc tgatgcccag atagaccgcc
      421 actccgccag tgcccctggg tcccccggat ccagcgactc aagcgcacgg atgagtgcat
      481 gggcgatcgg cgcactccag tcggcaggag cctcggcccg cgcgcggcgc aagacatcga
      541 ccgctctcac gccaccgacc tggccgagtg catcgaccgc ggccagggcc acttcgcggt
      601 cggaactcgc cgcgtgccgt accagcagcg gcacagcgcc ccgatcccgc ttcgccccga
      661 gcgcggtcaa cacacccacc aggggccgac cccggagcct gagggtcgcc gcccgcagcg
      721 ctgaggccgc cgccgggtcg tcgaggcgtt gcaacacctg gaccgcccgg gacccgagct
      781 caacatcgcc cagccatcgg caggccgtgg gtacttcggc ggccgtggcg atccgtgaga
      841 gctggtcgaa caggaagcga cgggcatccg gcgtggcatc cgcctcgatg gcggcggcga
      901 gcttggccgc cacgagagca cgccggcccg gatcggccgc cgcgcggcgc acgagcccct
      961 ccaccacagc caaccgatcc cggcggtcgg tgcattggta cccgagcacg ggcaacaggg
     1021 cgacatcgac gtcgtagggg tcagcgacag ttgattggcc cgtggcggcc cactcgacac
     1081 cacgagcgaa cagaagccgc cagccgtccg agtccatggc gaccccgtcg tgccccagca
     1141 cgatgctgaa gccacgcccg cgcccgtagt gtgtcgccac gaccatgggc tcgatttccc
     1201 ccgtgccgcc cgcctcaggc gtcgagtagg cggtgcacag caccttggcc cgtgggccca
     1261 gcgtcagccg ctgatagagc tcatcggtga tgcggaactc ggccaggccc ttggccaccg
     1321 ggtggtcccg caccgcgggc ttcacggcga atgtgtggat gcgggcgtgg ttcgccttgc
     1381 cctcttccca gtagatgccg atcaggtcgc ggtactgcgg ccaggcgggg aagcatgctg
     1441 tcgaggcgtg gatggcaacg aagccgcctc ctgcgcgaac gaaggccaac agcgccctct
     1501 gggcctcctc accccacggg cagtccggcg cggggtagtt cgaccagttg tcgacaacaa
     1561 ccgcatacga cgccaacgtc ccgggccgta gcgtcgatgg gtcttcggtc acgtcgacag
     1621 cgaagcgtcc cgtgtcctcc agaatgcgct tcacgaggcg tgtcgactgc ccgggctcca
     1681 gggcggcctg ggcctcgtgg gtgttcagtc caccgagcag cagcacgcgg atgggcgcaa
     1741 ccggccccgc atgcagagcc gcggagctga ggagcagtgc cgccaatacg ctcagtgccc
     1801 gcgatccgag tctgcccacg gacatggagt cacccccgct agggaagccc agagagaccg
     1861 tgcggccgct tcgtgttcac gtggagatgg gcgatggatc ggctcggcac ctgcgccgtg
     1921 ggcgacgaga caagaggcgc agaccccgtg ccggggtctg cgcctgcgtc agacaatgtt
     1981 ggtggagcgt accggtcgca tgcgacgtgg cgagctactc gccagagccc gcgcccgcgg
     2041 cttggttctt catgcccccc atggcaccgg gcatcttgcc gcttccggcg acgccagaag
     2101 cttgaccggc cttctgcatg gcgtcgcgtg tcgacttgac ggcgtcgaac atctcgccct
     2161 tcctctgctc gctggccgac ttcttggccg tgactttctc gggctccttg gccttggagc
     2221 tgcagccgac caggacgctt ccgcagaaaa ggaccatgac tccccaggcg atgaaccgcc
     2281 tcatctggca ctctccttcg cgtcgggaca ctctcgctca gtgctccggg ggaccggcta
     2341 gcgatccaca ccgtccgtat agtagatgcc gctccccttg cccgtgtagt tcttcaccag
     2401 ctcgtgcttc tgccacttca catgcccatc ggcgtaggcc agattggtac cgccattgtg
     2461 gcgcgcctgg atccagtcga ctcggctacc ggcgaatgcg cagctcccgt tgggcgtggc
     2521 cggcgcgttg aggcactcga tgaacatcat cacgctggcc ggagccgtga tgttcgccat
     2581 cgcctgctgg ctcatggtca tgcacgggta cccgtagtcg ccgtagggca gctcctagca
     2641 gcacggagcg cagttgtggc cggtggtcga cgggcacagc cagatctgct tgttcttgat
     2701 gtagggctgc agataccctg tggtgtagaa gcaccctggg gggttcttgc accacgaggc
     2761 accgtcgcca cagaagggca tgaagcgttc gtcgtagtca ttggcgtaca tcaggttgcc
     2821 cagggcgatt tgcttcaggt tagaggcgca ggtggtctgg cgggccttct ctctggcctt
     2881 ggcgaatacc gggaacaaga tggctgccag aatggcgatg atcgcgatga ccacaagcag
     2941 ttcgatcagg gtgaagccgc gccgccgcat gaccatccct ccgcaactga gtctggagca
     3001 ccgaccaatg ccacataacc tgagtaatat tcgcgcagat tcccccagaa cctccagacc
     3061 gcacacctcg tggacccccc agtatggcgc gctcggaggc cgcgcccgct cgccgcgacg
     3121 tggactggat gcagaaggcg gcgcggggct gaggaaaggc gcggcttggg taccgcgcac
     3181 catggaggcg gcctgagcac gattccggcc tgtcaccggt tctcggctct gttgggcact
     3241 ggcgtcgcca gggtcgcgca gaggagttcc cagagcgtga gcagatccag cgcgttgtgc
     3301 tggaggatgg gcacgagcag ctcggcatcc tgtgtggcca cgaactcgtg gtagacggcc
     3361 ggaatgaggt cgctcggcac atcgtcggtc cggtggcggc cgcagagctc gcgctcgagc
     3421 gtctgcagcc ggcagttctc gaaacggccc ctccagcggc ggcgggccgc gtggaggatg
     3481 tcgatgtgca ccggtggcgg gtcaaagcgt agccggtggt agcgcatgcg gtcggcgagg
     3541 aagggcacgt cgaaggactt gccgttgaac gtcacgatgg cgcgggtccg ggccatgtgc
     3601 gcggccaggg cgtccagcat ggcgggctcc tcgtcgtagt cgcgggccag gaagaagcgc
     3661 agccggggtg cccccgacag gaagtccagc actccggcga ggaacagcgt ggcgcccatc
     3721 agcccggcgg tctcgatatc gaagacgagc agatccgagg gctcgaggcc ctgcatctcg
     3781 gggatgtcgg gcgccgcgcc cggacgcgtc gccaggtcgt caagcagagc gcgggcacgc
     3841 ggccaaacgc cctcaacctc gagctcggtc agccagaagg ggccgaagtc ggcgtcgatc
     3901 tcgcccccgc cgagcagatc gacgaactcg tcgccctcgt cagccacggg gaagaagacc
     3961 ggcgaggagt cctcggggat gtcccacggc gcggggcggg acggccgggg cggccggacc
     4021 tgcggcgcag cgccatcggc cggcggcggg atggcgccga tccgtgagag cttccgtcgc
     4081 aagtcgcgtg agagcaagtc gcgggccctc ctgtgggcgg gagaccgcgt cggtgatgcc
     4141 atctagccgc gccgtaccct ctgccacgcc agccggccca ggagccgaag ctgttcgcct
     4201 atgatagccg atgacatctt cgagtgcccg tggcggcgtt cggtgaaggt gatcggcacc
     4261 tcggcgatgc gcaggccaag ccgctgcgcg cggacgaggg tctgggtcag caacgcatag
     4321 ccccggccgg gcactgaggc cagatccagc cgctcgatgg cctcgg
//
LOCUS       DAOSPL010000159         4366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20132_length_4366_cov_2.653445, whole genome shotgun sequence.
ACCESSION   DAOSPL010000159 DAOSPL010000000
VERSION     DAOSPL010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20132_length_4366_cov_2.653445"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="PLQ54_04190"
     CDS             <1..852
                     /locus_tag="PLQ54_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HOS92487.1"
                     /translation="YTMKLRGVPVATGQVVIDRFLAIGVPPEDQQIHGIRCTDPAFGL
                     SAVWVTEADRMRAEMAGYTVVDPTSVIATHVTEVIKKHAAEILGRQEVQDLVERIRQT
                     RPAAVEGLIPTVLSVADLHRILQNLLAEQVSIRDMLSIMEALGDRAPQTRDIDALTEA
                     VRLGLRRRICAQYLTEDGRLPVLTLDPRVERLILAAVQEDASGQFLALEPDQARAILE
                     ELAKGMERASRMGYQPILVVTPRLRLVVRRLTASVFENLVVLSQAELVPDITIENLGM
                     VSLPSEG"
     gene            842..2107
                     /gene="flhF"
                     /locus_tag="PLQ54_04195"
     CDS             842..2107
                     /gene="flhF"
                     /locus_tag="PLQ54_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HOS92488.1"
                     /translation="MKVKKYQASSYRDALAQVRQDLGPDAVILTTREISRSRFLGIGR
                     RRAVEIVAAQDIRLREGRPDRHDRGRARPRAPERARPHVSSPAISRDPQPLASATPSA
                     LREPFGASSPAAAAAAGDPATRERLEELERHLSTMRVALDAVVHPAEPTAPVVPPLSE
                     PGPELGPLYRILCEADVSPALAAEIVLAAGNGSGGDGRPEAVRAQLVERLVTSGPIGR
                     PEGEKPRVVALVGPTGVGKTTTIAKLAGEYTFMHNCRVGLLTVDTYRVAAVEQLRTFA
                     KIMTVPVQVAASPREARECLDKFTRPELILVDTAGRSQKHDEQVRALQKLLAAVEPDE
                     VHLVLSATTKSRDLIDVVKRFREVGVDRMIFTKLDETNSYGPVLNVAVESGLPISYVT
                     NGQMVPEDIALATPGYLADLVMGGRTRDA"
     gene            2100..2993
                     /locus_tag="PLQ54_04200"
     CDS             2100..2993
                     /locus_tag="PLQ54_04200"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOS92489.1"
                     /translation="MLDQADGLRLLASCYRKHDQRMVRDPASRLVCVTGGKGGVGKTN
                     VAANLGLALCSLGSRVVLFDADLGLANLDMILGLEPRYTLEHVVRGQVEDLRDVVMDG
                     PGGLKVVAGGSGIAELANLPGAAVQRLLRGLMGLQELGDTVVIDTSAGIHQTVLSFIV
                     SAHETLLVTTPEPTAVAAAYATMKAVSTQIQDPRFFLVVNMARSDQEGQEAIDRLTHV
                     AKQFLGIRVRSLGIIPWDSRVSDAVRLRVPFVQQFPGCAASRRLRSVAEQLCTRKPQP
                     QPGVGLATAVDRLRKFFFARA"
     gene            3355..>4366
                     /locus_tag="PLQ54_04205"
     CDS             3355..>4366
                     /locus_tag="PLQ54_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92490.1"
                     /translation="MGQDYDIYLDLTYDDGTPLWGQTARFEPGTHDWQSAQFDFDPSK
                     PVRRIEVFVLFRKAKGTVWFDDIQVCLRPFAFTGLDLTPGLFGGTSLGITANTTMPAR
                     WRMAISGPGGTRAEQRGEGSRFRAIWAGSPRAAAGTYRVSLTARDGLRGEAIRETREL
                     ELGAGGPDGPYRLWIEDSMVRVLPDAFPPSRPGRPVASLSLAGNEYESFQLAFLAAPG
                     ARVEGLSLETSDLVGRSGARIAASEIEWHQVGFVHAERLLGHPAYPEASPGWWPDPLL
                     EVGRFSLEPGRTQAIWVTVHAPAGTPAGDYEGTLAIVQPGRPDTEVEVRAHVYGFDLP
                     VQG"
BASE COUNT          652 a         1464 c         1520 g          730 t
ORIGIN      
        1 tataccatga agctgcgagg tgtcccggtg gcgaccggcc aggtggtcat cgaccgcttc
       61 ttggccatcg gtgttccgcc cgaagaccag cagatccatg gcatccggtg cacggacccg
      121 gcctttgggc tttcggccgt ctgggtcact gaggccgatc ggatgcgggc ggagatggcc
      181 gggtacacag tggtcgaccc gacatcggtg atcgcgaccc atgtgacgga ggtcatcaag
      241 aagcacgcgg ccgaaatcct gggccggcag gaggtgcagg acctggtgga gcgcatccgc
      301 cagacacgcc ccgcagcggt cgaggggctg atccccaccg tgctcagcgt tgcggatctc
      361 caccgaatcc tgcagaatct gctggccgag caggtttcga tccgagacat gctcagcatc
      421 atggaggcgc tgggtgaccg ggcaccgcag acgcgcgaca tcgacgcgtt gaccgaggct
      481 gtgcggctgg ggttgcggcg tcgcatctgt gcccagtatc tgaccgagga cggccgcctg
      541 ccggtgctca ccctcgaccc cagggtggag cgtctgattc tggccgcggt gcaggaggat
      601 gcctcgggcc agttcctggc gctcgagccc gatcaggcgc gcgcgattct tgaggagctg
      661 gccaagggga tggagcgggc gtcgcgaatg gggtaccagc ccattctggt ggtcacgccg
      721 cgattgcgtc tggtggtccg gcggctgacg gcgtcggtat ttgagaatct ggtcgtgctt
      781 tcgcaagccg agttggtacc tgacatcacc atcgagaatc taggaatggt ctcgctgccc
      841 agtgaaggtt aagaagtacc aggcatcgag ctaccgtgac gcgctggccc aggttcggca
      901 ggacctgggg ccggatgccg tgatcctgac gactcgggag atcagccgga gtcgctttct
      961 gggcatcggg cggcggcggg cagttgagat cgtggccgca caggacattc gcctgaggga
     1021 gggccgcccc gatcggcacg atcgaggccg ggctcggccg cgggcgcctg agcgggccag
     1081 acctcatgtc tcgtcgcccg cgatctcacg ggacccccag cccttggcga gcgcgacgcc
     1141 cagcgctctg cgcgagccgt ttggggcttc gtccccggcc gccgccgcgg cggcgggaga
     1201 ccccgccacc cgcgagcgcc tcgaggagct cgaaaggcac ctgagcacca tgcgcgtcgc
     1261 cctcgacgcc gtggtgcacc cggctgagcc gacggcaccg gtggtcccgc cgctttccga
     1321 gccggggccc gaactcgggc cgctgtaccg catcctctgc gaggccgacg tatcccccgc
     1381 cttggcggcc gagatcgtgt tggccgccgg caacggatcg gggggtgatg ggcgccccga
     1441 ggccgtgcgc gcccagcttg tcgagcgttt agtcacctcc ggccccatcg gtcggcccga
     1501 gggcgagaag ccgcgcgtcg tggccctcgt cggtcccacc ggcgtcggca agacgacgac
     1561 catcgccaag ctggccggcg agtacacctt tatgcacaac tgccgcgtgg gcctgctgac
     1621 cgtcgacacc taccgggtgg ccgccgtcga gcagctccgc acctttgcca agatcatgac
     1681 cgtaccggtg caggtggcgg catctccccg cgaggcccgc gagtgcctgg acaagttcac
     1741 gcgccccgaa ctgattctgg tggataccgc cgggcgcagc cagaagcacg acgaacaggt
     1801 gcgggccctg cagaagctgc tggccgccgt cgagcccgat gaggtgcacc tggtgctgtc
     1861 ggcgaccacc aagtcccgcg acctcatcga tgtggtcaag cggttcagag aggtcggggt
     1921 cgaccggatg atcttcacca agctggatga gaccaactcc tacggtcccg tgctgaacgt
     1981 tgcagtcgag agtggccttc cgatatccta tgtcaccaat ggccagatgg tgccggagga
     2041 tatcgccctc gccacaccgg gatatcttgc ggaccttgtc atgggtggac ggactcggga
     2101 tgcttgacca ggccgacggc ctcaggctgc tagcgagctg ctaccgcaag cacgaccagc
     2161 gaatggtgcg cgatccggcc tcccgcctgg tgtgtgtaac cggtggcaag ggcggcgtcg
     2221 gcaagacgaa cgtggcggcc aacctcggtc tggcgctgtg ctccctggga tcccgcgtgg
     2281 tcctgttcga tgccgatctg ggcctcgcca acctcgatat gatcctcggc ctcgagcccc
     2341 gctacaccct cgagcacgtg gtccggggcc aggttgagga cctgcgcgac gtggtgatgg
     2401 atggtccggg tgggctcaag gtcgtggccg gcgggagcgg gatcgccgag ctggcgaacc
     2461 ttcctggtgc tgcggtgcag cggcttctcc gcggactcat gggtctgcag gagctcgggg
     2521 acaccgtcgt cattgacacc agtgccggga ttcatcagac cgttctctcg ttcatcgtca
     2581 gcgctcacga gaccctcctc gtgactacgc cggagcccac ggccgtggcg gcggcctatg
     2641 ccaccatgaa ggccgtctcg acccagattc aggatccgcg gttcttcctc gtcgtgaaca
     2701 tggctcggag cgaccaagag gggcaggagg ccatcgatcg cctgactcac gtggcgaagc
     2761 agttcctggg catccgcgtg cgctcgctgg gtatcatccc atgggattcc cgggtcagcg
     2821 acgccgtgcg tctccgagtc ccctttgtgc agcagtttcc cggctgcgcc gcctcgcgcc
     2881 gcctccgctc ggtagccgag cagctgtgca cgcgcaagcc gcagccccag ccgggcgttg
     2941 gtctcgccac cgccgtcgac cggctgcgga agttcttctt tgcccgggcg tagaggctcg
     3001 tgctgcacgc tgtcgtgctt gtcgcatgag tggaccggtc ggcatgcttt gcatggtgat
     3061 cgaggaggcc tccgtggaca ctcgcatcgc tccgtggcta ctgctggctc tggctctggc
     3121 cgcgtccgca tccgcgactg cccaggagcg cggcgcgaac ctggttcgca acccgagctt
     3181 cgaggacgac tcggtctggg aggccgtgag cgggggcttc gcatacgacg cgggcgtggc
     3241 gcactcgggg aagcgctcgg ttcgcgtctc cggtgatgac ctcgaggcca gctcgatccg
     3301 cccgttgcgc acccgttccg catctccggc tggagccggg ccgagggcgc cgaagtgggg
     3361 caggactacg acatctacct cgacctcacg tatgacgacg ggacacccct gtggggccag
     3421 accgcccgct tcgagccggg cacccacgac tggcagagcg cccagttcga cttcgacccg
     3481 tccaagcccg tgcgacgcat cgaggtgttc gtgctattcc gtaaggccaa ggggacggta
     3541 tggttcgacg acatccaggt ctgcctgcgg cccttcgcgt tcaccgggct tgacctgacg
     3601 cccgggctct tcggcggcac aagtctgggc atcacggcca acaccacgat gcccgctcga
     3661 tggcgcatgg cgatcagcgg ccccggcgga accagggccg agcagagagg cgagggctcc
     3721 cgcttccggg ccatctgggc gggctcgccg agggccgcag cgggcacata ccgcgtgagc
     3781 ctgaccgcga gggacggcct ccgcggcgag gccattcgcg agacgcgcga gctggaactc
     3841 ggagccggcg gccccgatgg tccctaccgc ctttggatcg aggacagcat ggttcgcgtg
     3901 ctgcccgatg cctttccgcc atcacggccc ggcaggccgg tggccagcct ctcgttggcg
     3961 gggaacgagt atgagagctt ccagctcgcg tttctggctg ctccgggcgc gcgggtagag
     4021 gggctgtcgc tggagacctc agacctggtg gggcgctcgg gcgcccgcat cgcggcctcc
     4081 gagatcgagt ggcaccaggt cgggttcgtc cacgccgagc ggctgctcgg gcacccagcc
     4141 taccccgagg cgagtccggg ctggtggccc gatccgttgc tcgaggtcgg ccggttctcg
     4201 ctcgagccgg gccgcacgca ggccatctgg gtgacggtcc atgcgcccgc gggcacgccc
     4261 gccggcgact atgagggcac cctcgccatc gtgcagcccg gccggccgga caccgaggtc
     4321 gaggtgcgcg ctcatgtgta cggcttcgac ctgcccgtgc agggcc
//
LOCUS       DAOSPL010000160         4365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20137_length_4365_cov_3.725058, whole genome shotgun sequence.
ACCESSION   DAOSPL010000160 DAOSPL010000000
VERSION     DAOSPL010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20137_length_4365_cov_3.725058"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1796)
                     /locus_tag="PLQ54_04210"
     CDS             complement(120..1796)
                     /locus_tag="PLQ54_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOS92491.1"
                     /translation="MRRVSRREFVQAMGAVGAAAAAGRPAPAVPKRRDRPNVVLILTD
                     DQGYGDLGCHGNDRIRTPNLDRFRAQSTEFTRFYCSPVCAPTRASLMTGRYNYRTGAI
                     DTYLGRAMMHPDEMTLAQMLRAGGYRTGIFGKWHLGDNYPMRPYDRGFEEALVHKGGG
                     MCQPADPPGGSYFDPWLDHNGTWERHQGYCTDVFTTAAIDWIGAHQPHEPFFCYLATN
                     APHTPVQVDDRYVRPYRELGFDDENAGVLGMITNLDENVGLLLDHLDRSGLADDTIVI
                     FMTDNGPQQARYVCGLRGLKGSVYEGGIRVPSIWRWPGRFEAGRALGVLAAHIDVVPT
                     LLDLCGVDPPDRVSLDGTSLAPWLSGEPTEWPDRILFFQWHRGDEPEPFRACAAVTQR
                     DKLVDGRELYDLEADPGETRDLAGERPERVAELRAAYQRWFTDVSSTRGYAPPRIVVG
                     TRFENPSTLTRQDWRGPRAGWGPDDLGHWEVEVAEAGEYDITLRFAASAGNVAATLRL
                     GPIRCTEAIPPGAGQLTFSQVPLRSGPARLEAWVARSGSQVGVEYVEVRR"
     gene            1938..2618
                     /gene="queC"
                     /locus_tag="PLQ54_04215"
     CDS             1938..2618
                     /gene="queC"
                     /locus_tag="PLQ54_04215"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOS92492.1"
                     /translation="MTRALAVVLMSGGMDSCVCAAIAVLECELAALHVTYGQRTADRE
                     REAFEAQADHYGVPASRRLVTSLPSLATMGGSALTDPLIPVPEGGLEPEAIPVTYVPF
                     RNAHLLATAVSWAEVLGAQRVVIGAVQDDSSGYPDCRVEFYEAFRRVIEAGTRPETRI
                     AIDTPVIHLSKAEIVRRGIELGAPLELTWSCYQRTDVACGVCDSCRLRLRAFEQAGVR
                     DPVPYASD"
     gene            2655..4001
                     /locus_tag="PLQ54_04220"
     CDS             2655..4001
                     /locus_tag="PLQ54_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS92493.1"
                     /translation="MTVGDGGVPVAADSDVLVVGGGTAGVVAALAAARNGANTVLIEN
                     LGFLGGSQSAALVTPIMDFHCDGKPLITGIHLEMIDRVNALGENDGVWHNPEVLKRVY
                     EDMCAEASVELRYYTQAVDVLMSGKSIVGVLAAAKAGLEGFLAKQVIDCTGDADVAVA
                     AGVPCQKGRESDGMNQPASLRFIAGGVDAERLASFLRGRGHPVPDLAHIEVGYAKGGA
                     QNLGDLWEAGAARGEIPAGDEGVYIQFFALPGCPGLVAFNCPRVCRVDGTDSASLTWA
                     AIEGRRRIEKLLSFCRRNIPGFEKAFVALSAPMVGIRESRRIEGEYTLTVHDFMAAAK
                     FPDAVAKSRYPVDIHNPDREGVTVITLPPGDYHDIPYRCLVPKVVNQLLVAGRCVSAT
                     FEAQAAIRVQANCRALGEAAGTAAAMCAAEGVTPRRLNRPKLIERLRAQGARVGAD"
     gene            3998..>4365
                     /locus_tag="PLQ54_04225"
     CDS             3998..>4365
                     /locus_tag="PLQ54_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOS92494.1"
                     /translation="MSARRFGFHLSIAGGLQNVPARARRTRCDTIQIFTQSPRRWSGP
                     APTSDAIAAFRQGIAAQGIDPVTIHTIYPINLASADDGLYEGSIDLVAEDLRRAGLLG
                     VPLVNTHVGHARDLNYDEAL"
BASE COUNT          675 a         1446 c         1520 g          724 t
ORIGIN      
        1 ctccgcggct cgctccgggt cgtttcgctc ccaggtgcgg gccggcttgc gcccttcgcg
       61 cttcgcgccc cccttccgtc cggccctccc ccagatacgc aggcgcccga ccatcgatgc
      121 taccgacgca cctcgacgta ctccacaccc acttggctgc ccgaccgggc cacccaggct
      181 tcgaggcggg caggaccgga ccgtagcggg acctggctga aggtgagctg cccggcccct
      241 ggcggaatgg cttcggtgca cctgataggg cccaggcgca gagtggccgc cacgttcccg
      301 gcggacgcag cgaagcgcag ggtgatgtcg tactcgccgg cttcggcgac ctcgacttcc
      361 cagtggccca ggtcgtcggg cccccatccg gcgcgaggac cccgccagtc ctgccgcgtc
      421 agtgtggacg ggttctcaaa ccgtgtcccg accacgatgc gcggcggggc gtagccgcgg
      481 gtgcttgaga cgtccgtgaa ccaacgctga taggcggcgc ggagctcggc cacgcgctcg
      541 gggcgctccc ccgcgaggtc gcgggtctcg cccgggtcgg cctccaggtc atagagctcc
      601 cggccatcga ccagcttgtc tcgctgggtc acggcggcac aggcgcggaa gggttcgggt
      661 tcgtcgccgc ggtgccactg gaagaagagg atccgatccg gccactccgt gggttcgccg
      721 ctcagccaag gtgcgaggct ggtgccgtcg aggctcaccc ggtcgggagg atccacaccg
      781 cagaggtcga ggagcgtggg aacgacgtcg atatgggccg ccaggacacc gagcgctcgg
      841 ccggcctcga accggccggg ccagcgccag atcgacggca ctcgaatgcc gccctcgtag
      901 accgagccct taagcccgcg gaggccacag acgtagcggg cctgctgggg accgttgtcc
      961 gtcatgaaga tcacgatggt gtcatcggcc aggcccgagc ggtccagatg gtcgagcaac
     1021 aggcccacgt tctcgtcgag attggtgatc atgccaagca cgcccgcgtt ctcgtcgtcg
     1081 aacccgagct cgcggtaggg ccggacgtag cggtcatcga cctgcacggg cgtgtggggc
     1141 gcattggtgg ccaggtagca gaagaagggc tcgtgcggct ggtgggcgcc gatccagtcg
     1201 atggccgcgg tggtgaagac atcggtgcag tatccctggt ggcgctccca ggttccgttg
     1261 tggtcgagcc acggatcgaa gtaggagccc cccggcgggt cggcaggttg gcacatcccg
     1321 ccgcccttgt ggaccagggc ttcctcgaag cctctgtcgt agggccgcat ggggtagttg
     1381 tctcccagat gccacttgcc gaagatcccc gtgcggtagc cccccgcgcg gagcatctgg
     1441 gcgagggtca tctcgtcggg atgcatcatt gcccggccca ggtaggtatc gatggcgccg
     1501 gtgcggtagt tgtagcgccc ggtcatgagg gaagctcggg tgggtgcgca cacgggacta
     1561 cagtagaagc gcgtgaactc cgtgctctga gcgcgaaatc ggtcgaggtt cggggtacga
     1621 atgcggtcgt ttccgtgaca tccgagatcc ccatagccct ggtcatcggt gaggatcagt
     1681 accacattgg gccggtcgcg ccgtttcggc acggctggcg cgggccgacc tgccgccgcg
     1741 gcggctccta ctgcccccat ggcctggacg aactccctcc gcgagacccg gcgcaccccc
     1801 agcctcctgc tcgctggcta ccggatgcgc cttctggagc aaggaccctc agtcctgcga
     1861 ggccgaaggc agtccggggt cccatggagt tcccaggggg gcaccggccg gtcacaatag
     1921 agcctggagg gtgagacatg acgagggcgc tggcggttgt cctgatgagc ggtgggatgg
     1981 atagctgcgt ctgcgcggcg atagcggtgc tggaatgcga gctggccgct ctgcacgtga
     2041 cctacggcca gaggacggct gaccgcgaac gcgaggcgtt cgaggcgcag gcggaccact
     2101 atggagtgcc ggcgagccgg cggcttgtga cctcgctgcc gtcactggcc accatgggcg
     2161 gctctgccct gaccgatccg ttgatccccg tacccgaagg cggactcgag cccgaggcca
     2221 ttcccgtgac ctacgtgccc ttccgcaacg cccatctact ggccacggcc gtctcgtggg
     2281 ccgaggtgct tggggcccag cgcgtcgtca tcggcgcggt gcaggacgac agctcaggct
     2341 accccgactg ccgggtcgag ttctacgagg cattccgccg cgtcatcgag gcaggaaccc
     2401 gtccggagac gcgcatcgcc atcgacacac cggtcattca tctgtcgaag gctgagatag
     2461 tccgccgggg tatcgaactc ggcgcgccct tggaactcac ctggtcgtgc tatcagcgga
     2521 ccgacgtggc ctgcggggtg tgcgatagct gccggctgcg gctccgggcg ttcgaacagg
     2581 ccggcgtgcg cgacccggtc ccctatgcct cagactgagg agaagccatg actctgcgca
     2641 gcaggaatta ccgagtgact gtgggtgacg gaggtgtgcc cgtcgccgcc gacagcgacg
     2701 tgctggtcgt tggggggggc accgcgggcg tcgtcgccgc actggccgct gcccgcaacg
     2761 gcgccaacac cgtgctcatc gagaacctgg gcttcctcgg tggcagccag tcagccgccc
     2821 tggtgacgcc catcatggac ttccattgcg acggcaagcc gctgattacg ggcattcacc
     2881 tcgagatgat cgaccgcgtc aacgcgctcg gtgagaacga cggggtgtgg cacaaccccg
     2941 aggtcctcaa gcgtgtgtat gaggacatgt gcgctgaggc ctccgtcgaa cttcggtact
     3001 acacccaggc cgtcgatgtg ctgatgtccg gcaagtccat cgtgggcgta ctggcggcgg
     3061 cgaaagccgg actcgagggc tttctcgcca agcaggtgat cgactgcacc ggcgatgccg
     3121 atgtggccgt ggcagccggc gtcccgtgcc aaaaggggcg cgagtccgac gggatgaacc
     3181 aaccggcctc gctccggttc atcgccgggg gcgtggatgc cgagcgtctg gcctccttcc
     3241 tccgcggacg gggccatccg gtgccggatc tggcccacat cgaagtcggc tatgccaagg
     3301 gcggggcgca gaacctgggc gacttgtggg aggcgggcgc cgctcggggg gagatccccg
     3361 ccggcgatga gggcgtctac atccagttct tcgcactccc cggctgtccc gggttggtgg
     3421 cgttcaactg cccacgcgtg tgccgcgttg atgggactga ctcggcctcg ctgacctggg
     3481 cggccatcga gggccgaagg cgcatcgaga agctgctgag cttctgtcgg cggaacatac
     3541 ctggtttcga gaaggcgttc gtggccctat cggctcccat ggtcggcatc cgcgagagcc
     3601 ggcggatcga gggtgagtac actcttaccg ttcacgactt catggccgcg gcgaagttcc
     3661 ccgatgccgt ggccaagtca cggtacccgg tggacatcca caacccggat cgcgagggag
     3721 tgacagtgat aaccctcccg ccgggcgatt atcacgacat accttaccgc tgtctcgtgc
     3781 ccaaggtcgt caaccaactg ctggtggcgg gacggtgcgt ctcggccacc ttcgaggccc
     3841 aagcggccat ccgagtgcag gccaactgcc gggcgttggg tgaggccgcg ggcacggccg
     3901 ccgcgatgtg tgcggctgag ggtgtcacgc cccgccggct gaaccggccg aagctgattg
     3961 agcggcttcg tgcgcaaggt gcacgggtgg gcgccgattg agcgcccggc gtttcggctt
     4021 ccatctatcg atcgccggtg ggctccagaa cgtccccgcc cgggcgcgcc gcacccggtg
     4081 cgacaccatc cagatcttca cccagagccc aaggcgctgg tcgggcccgg cccctacctc
     4141 tgacgccatc gccgctttcc gccagggcat tgcggcgcag ggcatcgatc ccgtgacgat
     4201 ccataccatc tatccgatca acctggcgtc ggccgatgac gggctctacg agggctccat
     4261 cgacctggtg gccgaggatc tgcggcgggc cggtctgctg ggcgtgcctc tggtgaacac
     4321 acatgtgggt cacgccaggg acctgaacta cgacgaggcg ttgga
//
LOCUS       DAOSPL010000161         4364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20142_length_4364_cov_3.148526, whole genome shotgun sequence.
ACCESSION   DAOSPL010000161 DAOSPL010000000
VERSION     DAOSPL010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20142_length_4364_cov_3.148526"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="PLQ54_04230"
     CDS             <1..1005
                     /locus_tag="PLQ54_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOS92495.1"
                     /translation="ATQRALRSAERDRAALWAAANATGDGDWQQRTAELRDTLWDDVI
                     GRLPPASLPANPRARQVYDEPTWAGFDVVLDVWPDVFASGILLVPKDLKPGERRAVVV
                     CQHGLEGHAADVVGPVAEQAYRDFARQLVELGYVVFAPQNPYLGEDAFRVLQRKANPL
                     GYSLFSVIVRQHERILEWLGTLPYVDAGRIAFYGLSYGGKTAMRVPALLPGYCLSICS
                     GDFNEWIVKNASYDQSMSYVYTGEYEMFEFDLGRTFNYAEMAALIAPRPFMVERGHDD
                     GVSVDEWVAYEYAKVRRFYTKRGFADRTEIEFFDGGHWINGVGTFEFLRRHLGWPAAD
                     "
     gene            1184..2356
                     /locus_tag="PLQ54_04235"
     CDS             1184..2356
                     /locus_tag="PLQ54_04235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOS92496.1"
                     /translation="MPRTIVHVDMDAFYAAIEQRDHPELRGKPVIVGGRPGSRGVVSS
                     ASYEARAFGVRSAMPSTEAQRLCPEAIFAPVDMPKYAEASRTLMKVLESFSPTIEPLS
                     IDEAFLDASGTERLFGPPEELGRAIKCRVLETLQITASVGIAPNKFLAKLASDLRKPD
                     GLVVVGEGEAPALLDDLPIRKLFGVGEATEERLQRLGIATIGQLARYPRDILIQHFGQ
                     AGEHLSDLANGIDERPVETEREARSLSTEVTFDEDIADYERLEAVLFDLSEQVAWRLR
                     KQRLRARTFTLRVRFADFDTVSRSRSQKRASNHGPLIFRSLCEPLAGLRESRRAVRLL
                     GVAATQLCGEGDEAQLSLFGDDELEKARAVDEVLDAISARFGRGTIGRGRTLRPDS"
     gene            2437..3429
                     /locus_tag="PLQ54_04240"
     CDS             2437..3429
                     /locus_tag="PLQ54_04240"
                     /inference="COORDINATES: protein motif:HMM:NF040257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HOS92497.1"
                     /translation="MSHTQPIRLLHYGLGPIGLGVVRQVADRSDMVSVAGVDIRPEVA
                     GKDVGELAGLGRDLGASVTATVGEAVGGAQVAVVCTGSHLSDVEPQIMELVQAGLNVI
                     STCEELSYPFWHHPEESRRIDDAAKRSGVTVLGTGVNPGFVMDLLPLIASGVCLRVDS
                     VYVRRVVDAGTRRGPLQAKVGAGMSVEEFRAGVQTGKLGHKGFVESVAMIADALELGV
                     DTISENIQPKLLDRSFGPWRAGQVAGIDQTATGLRQGEAVVSLRLLMYVNADDPRDRI
                     VLRGVPEVRWTLEGGTPGDQATAAITVNTIRRVLAAPPGLTSMKDLPPAHWARG"
     gene            3510..>4364
                     /locus_tag="PLQ54_04245"
     CDS             3510..>4364
                     /locus_tag="PLQ54_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92498.1"
                     /translation="MALLLTCLLGTQAVGFVYRPSTGRFKDGFVLWNEGRFYLYSMYM
                     DAPGDDSHFRNVWLATSPDGVHWQDVGPVISNAPFNIWAMAVHRVGDRFIMNHGSFSQ
                     PGLQNVIRFWKSMDLVHWTYMGSETDLFPDTRWYDPNSRLDCMSVVRALEDGHARYFG
                     YATGPGGFLESDDGLHWSGMPEPHFDWGTVPPPPMSPEEGVFEVGGCHEIGGRYYYLG
                     GWFNYMGTTGYGVYTLTADTPRGPFRPDEAAYRLCGNSGRWVALWARFCPTDRELLVN
                     GYMYSGYTY"
BASE COUNT          665 a         1445 c         1469 g          785 t
ORIGIN      
        1 gccacccagc gcgccttgcg gtcggccgag cgcgaccgcg cggcgctgtg ggctgccgcc
       61 aatgcgaccg gtgacggcga ctggcagcag cgcacggctg agctacgcga cacgctgtgg
      121 gatgatgtca tcggccgcct gccgccggcc agtttgccgg cgaacccgcg ggcccggcag
      181 gtctacgatg agcccacatg ggccgggttc gatgtggtgc tcgacgtctg gcccgatgtg
      241 ttcgcctcgg gcatcttgct tgtgccgaag gacctgaagc ccggcgagcg ccgtgccgtg
      301 gtcgtgtgcc agcatggcct cgagggccat gcggccgatg tggtgggacc ggtggccgaa
      361 caggcctatc gcgacttcgc ccgccaactg gtcgagctgg gctatgtggt cttcgccccg
      421 cagaatccgt atctgggcga ggacgccttc cgggtcctcc agcggaaggc caatccgttg
      481 ggctactcgc tcttctccgt catcgtgcgg caacacgagc gcatcctcga gtggctgggc
      541 acgctaccct acgtggacgc cgggcgtatc gccttctatg ggctatcgta tggtgggaag
      601 accgcgatgc gggtgccggc tctcctgccg ggctactgcc tctccatctg ctcgggtgac
      661 ttcaacgagt ggatcgtcaa gaacgcgagc tacgaccagt ccatgagcta tgtctataca
      721 ggcgagtatg agatgttcga gttcgacctc ggccggacgt tcaactacgc ggagatggca
      781 gcgctgatcg cgccgaggcc cttcatggtc gagcggggcc acgatgacgg tgtctcggtc
      841 gatgaatggg tggcctacga gtacgccaag gtgcggcggt tctacacgaa gcgcggcttc
      901 gcggatcgca ccgagatcga gttcttcgac ggcggccact ggatcaacgg cgtgggcact
      961 ttcgagttcc tgcgccggca cctcggttgg cctgcggcgg attgagcccg gccgaccgga
     1021 accggttcgc ccggtggtca cggcggggaa tcctgcctcg cggcttcgcc ccaccgctct
     1081 gggcaggtcg gcctgtatga cccgccgaag gcccgatggc ccaatgcccc gcccgcggcg
     1141 tgccgaaccg ggcggagaac ggcgcggttg gaccggggac accatgccgc ggactatcgt
     1201 acacgtcgat atggatgcct tctacgccgc catcgagcag cgggaccatc ccgagctgcg
     1261 cggcaagccg gttatcgtcg gaggtcggcc gggcagcagg ggggtggtct cctccgcctc
     1321 ctacgaggcc cgcgccttcg gtgtccggtc tgccatgccg tccaccgagg cgcagcggct
     1381 gtgccctgag gccatcttcg caccggtcga tatgcccaag tacgcggagg cgtcccggac
     1441 gctcatgaag gtcctcgagt cgttctcgcc gaccattgag ccgctatcga tcgacgaggc
     1501 gttcctcgac gcatccggca cggaacgcct gtttggccct cccgaggaac tcggccgagc
     1561 gatcaagtgc cgcgttctcg agaccctcca gatcacggcg tcggtgggca ttgcgccgaa
     1621 caagttcctg gccaagctcg cctccgatct ccgcaagccg gatggcctgg tcgttgtggg
     1681 cgagggtgag gcccccgcgc tgctcgacga tctgccgatc cgtaagctgt tcggagtggg
     1741 cgaggcgacg gaggaaagac tccagcgcct gggcatcgcg actatcggcc aactggcccg
     1801 gtacccgcgc gacatcctca tccagcactt tggtcaagcc ggggagcatc ttagcgatct
     1861 ggccaatggg atcgacgagc gtccggtgga gaccgagcgc gaggcccgga gtctgtccac
     1921 cgaagtcact ttcgatgagg acatcgccga ctatgagcgg ctcgaggccg tgctgttcga
     1981 cctatcggag caggtggcgt ggcgcctgag gaagcagcgg ctccgcgccc gtacgttcac
     2041 gttacgcgtg cgtttcgcgg acttcgacac cgtctcgcgc tcgcgcagtc agaagcgcgc
     2101 ctccaaccac ggtccgctga tctttcgctc cctgtgcgag ccgctcgccg gcttgcgcga
     2161 gagccgccgc gcggtccggc ttcttggtgt ggccgccacc cagctctgcg gcgagggcga
     2221 tgaggcccag ctgtcgctct ttggcgatga cgagctcgag aaggctcgcg ccgtcgatga
     2281 ggtgctcgac gccatctccg ctcgtttcgg ccgtggcacg atcggccgag gccgcacctt
     2341 gcgtccggac tcctgaccct gcatcatgcc cgtgtgtcct gcggagaccc atagtagggg
     2401 cgtccgccac tctctgccga aggagtgctg tagcccatga gccataccca gcccatccga
     2461 ctcctccact atggccttgg acccattggc ctgggtgtcg tccgccaggt ggctgatcgg
     2521 tctgatatgg tgagcgtggc cggcgtcgac atccgtcccg aggtcgccgg taaagacgtg
     2581 ggcgagctcg ccgggctggg ccgcgacctc ggtgcctccg tgaccgcaac cgtcggcgag
     2641 gccgttgggg gagcgcaggt tgcggtcgtt tgcaccggct cccatctctc cgacgtggag
     2701 ccccagatta tggaactggt gcaggctggg ctcaacgtga tttcgacatg tgaggagctc
     2761 tcatacccgt tctggcatca tcccgaagag agccgtcgga tcgacgatgc ggccaagcgc
     2821 agcggtgtga ccgttctcgg caccggtgtg aatcccggtt tcgtcatgga cttactcccc
     2881 ctgatcgcgt ccggggtgtg cttgcgggtc gactccgtgt acgtgcggcg tgtcgtcgat
     2941 gcaggcacgc gccgcgggcc cctccaagcc aaggtgggag ccggcatgtc tgtcgaggag
     3001 tttcgcgccg gggtccagac cggcaagctg gggcacaagg gcttcgttga gtccgtggcc
     3061 atgatcgcgg acgccctgga actcggggtg gacaccatat cggagaacat ccagcccaag
     3121 ctgctcgacc ggtccttcgg cccctggagg gccggacagg tcgcgggcat cgaccaaacc
     3181 gccaccggcc tccgccaggg cgaggccgtg gtgtccctgc gccttctgat gtacgtcaac
     3241 gcggacgacc cgcgcgaccg catcgtgctc cgcggcgtgc ccgaggtccg ttggacactg
     3301 gagggcggca cccccggaga ccaggcgacg gcggccatca ccgtcaacac catccggcgt
     3361 gtgctcgctg ccccgcccgg actcacctcc atgaaggacc tgccacccgc ccactgggcc
     3421 agaggatgat ggactcgtca cttctcgtct ccgctgccct cgccgttggg cggatgtcgg
     3481 atcgccactc gggttgaggt gagtcggtat tggcactatt gctgacgtgc ctgctgggca
     3541 cccaagctgt cggctttgtg taccggccgt cgaccggccg gttcaaggac ggcttcgtgc
     3601 tgtggaatga ggggcggttc tacctctaca gcatgtacat ggacgcgccc ggggatgact
     3661 cccacttccg caacgtctgg ctggccacgt cgcccgatgg cgtgcactgg caggacgtcg
     3721 gcccggtcat cagcaacgcg cccttcaaca tctgggccat ggccgtccac cgggtcggcg
     3781 accggttcat catgaaccac ggcagcttct cgcagccggg cctacagaac gtgatccgat
     3841 tctggaagtc catggacctg gtccactgga cctacatggg cagcgagacc gacctgttcc
     3901 ccgatacccg ctggtacgat cccaacagcc ggttggattg catgagcgta gtgcgcgcgc
     3961 tcgaggacgg ccacgcgagg tacttcggat atgcgaccgg tccgggcggt ttcctcgagt
     4021 ccgacgacgg gctgcactgg tccgggatgc ccgagccgca cttcgactgg ggcacggtgc
     4081 ctccgccccc gatgtcgccc gaagagggtg tgttcgaggt cggcgggtgc cacgagatcg
     4141 gcggcaggta ctactacctc ggcggctggt tcaactacat gggaaccacg ggctacgggg
     4201 tctacacgct caccgcggac acgccgcgtg gtccgttccg ccccgacgag gcggcgtacc
     4261 ggctgtgtgg caactccgga cggtgggtcg cgctgtgggc gcgcttttgc ccgaccgacc
     4321 gagagctgct cgtgaacggc tacatgtaca gcggctacac ctat
//
LOCUS       DAOSPL010000162         4344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20261_length_4344_cov_2.982513, whole genome shotgun sequence.
ACCESSION   DAOSPL010000162 DAOSPL010000000
VERSION     DAOSPL010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20261_length_4344_cov_2.982513"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..2131)
                     /locus_tag="PLQ54_04250"
     CDS             complement(20..2131)
                     /locus_tag="PLQ54_04250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOS92499.1"
                     /translation="MASRINAARGSHVPARPSTGRLVLHWAGRVLIALEIAVLVLVVG
                     VAGAVYGAYRGARKMLPSGTDIAGYRAPSASRIYASDGQLLAVVFDQDEGYRELVPLE
                     AIPEHVREAHIAIEDRRFYQHQGVDMPRILRVAYQNYRSGRIEAGASTLTQQLARAIF
                     LSARASYGRKAQEALLAIQIEREFSKDEILEMYLNQVCYGHPAYGIQAASRLYFDKDV
                     GQLSVAEGALLAGIVWKPTEVSPWVNRDAALRRRSLVLAAMARDGYITQAEAKRADDE
                     PLRLAPRRPSGLFSYRHPYFVTQAISELVQRYGPDAVYRSGLRVYTTMNEKVQSHCEE
                     AIRRGVQRARYMNCTQAATVMMEPNTGRILAIVGGTGFGPSDQNNRATNALRQPGSSF
                     KAFVYTAAIEKGYTPQTGISGSTVTIKAGPGEYWTVKSNGGSSLSLASALAASVNPAS
                     ARLVAALGPSRVVEVAHRMGIRTELEPVYSIALGTEEVTLLEMTTAYCGFANGGYRVE
                     PTTIDRVYDHDGVLLDEPAPRRVRAISMQTAYVMNGMLQGVVRHGTGTAASGLGVPTA
                     GKTGTTDDNKDAWFVGLTPQIVCGVWAGNDARPMSGSAFGGRVCAPMFKDMVGYALDV
                     IGKRKDLEFPGPGDERARAAIRFDSALFSGAQKEESKETKPLQRDLIDLTGGGGGGGR
                     GGAVPRARALSPSVPSYVRTP"
     gene            2548..3246
                     /locus_tag="PLQ54_04255"
     CDS             2548..3246
                     /locus_tag="PLQ54_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOS92500.1"
                     /translation="MSRLRAVFLDRDDTISRVSPVQAQAMYRAIGEIVGVPDFSITND
                     DYTALFTRTMAVPGIAPVNTPEREEAFWLRWMQFIFEDHGLTEGAAELSAEVRRRFVF
                     HTMMEPFPETREVLQALKDRGLKLGVISDTYPSLKASLESMGIAHYLDSIVASMVVGI
                     SKPDPRIFEIATNALGVKPQESVFVDDLQRNVDGARAAGMTAFRIDRSAATDFSGGVI
                     ASLRDLIAFADGAA"
     gene            complement(3255..4103)
                     /gene="nadC"
                     /locus_tag="PLQ54_04260"
     CDS             complement(3255..4103)
                     /gene="nadC"
                     /locus_tag="PLQ54_04260"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018672293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOS92501.1"
                     /translation="MNDIAGLPAVRRLIDLALEEDVAHGDITTMATVPATVYADAVIQ
                     AKQRGVVAGLGIAEAVFLRVDPSLGFAAVAADGDLVEPGDRLAEVSGRARSILTAERT
                     ALNFLQQLSGVATATRTMRLLIEGTRARLVDTRKTVPGLRVLQKYAVRVGGGSNHRFH
                     LADAVLIKDNHIAAAGGIGPAVRAARDLCPHTMRVEVEVQSLSQLREALEAGADVIML
                     DNMDCAMMRQAVELTAGRALLEASGGIREETVAEVARTGVDLVSCGAITHSAPALDIS
                     LDLVAR"
     gene            complement(4107..>4344)
                     /gene="nadA"
                     /locus_tag="PLQ54_04265"
     CDS             complement(4107..>4344)
                     /gene="nadA"
                     /locus_tag="PLQ54_04265"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOS92502.1"
                     /translation="DAQEFIIATEVGLIYRLERENPGKRFYCPSYVAVCPNMKMNTLE
                     NVLSALETLAPRITVPDDIARRAHRAIDRMLELV"
BASE COUNT          720 a         1504 c         1395 g          725 t
ORIGIN      
        1 ttggtggccg gatgtggcgc tagggggtgc ggacgtacga tggcaccgat ggcgagagcg
       61 cccgagcccg cggcacggca ccacccctgc ccccaccgcc tccgccaccc gtaaggtcga
      121 tgaggtctcg ctgaagcggc ttggtctcct tagactcctc tttctgagcg ccggagaata
      181 gagcggagtc gaaccggatc gcggcgcggg cgcgctcgtc gccagggccc ggaaactcca
      241 ggtccttcct cttgccgatc acgtccagtg cgtatccgac catgtccttg aacatcggcg
      301 cgcagacacg ccccccgaac gccgagcccg acatgggtcg tgcgtcgttg ccggcccaga
      361 ccccgcagac gatctgcggc gtaaggccca caaaccaggc atccttgttg tcatcggtcg
      421 tccccgtctt gcccgcggtc ggcacgccga gccctgaagc cgcggtgccg gtcccgtgac
      481 gcacgacccc ttggagcatg ccgttcatca cgtaggcggt ctgcatcgag atggcgcgca
      541 cgcgccgggg ggcgggctcg tccaacagca cgccgtcatg gtcgtatacg cgatcaatgg
      601 tggtgggctc gacccggtag ccgccattgg cgaagccgca gtaggccgtg gtcatctcga
      661 ggagcgtgac ctcctccgtg cccagcgcga tcgagtagac aggctccagt tcggtgcgga
      721 tccccatccg gtgcgccacc tcgaccaccc ggctcggacc cagggcggcc accaggcgcg
      781 ccgatgcggg gttgaccgat gcggccagcg cactggccag cgacagggat gaccccccgt
      841 tcgacttcac cgtccaatac tcgcctggcc ccgccttgat cgtcaccgtc gagccgctga
      901 tacccgtctg cggcgtgtaa cccttctcga tggccgcagt gtagacgaag gccttgaacg
      961 agctcccagg ttgccggagc gcgttggtgg cacggttgtt ctggtcggaa ggaccaaatc
     1021 cagtgccccc aactatggcc agaatgcggc ccgtgttggg ctccatcatc acggtggcgg
     1081 cctgagtaca gttcatgtag cgagcacgct gcaccccacg gcggatggct tcctcacagt
     1141 gggactgcac cttctcgttc atggtcgtat agacccgcag gccgctccga tagaccgcgt
     1201 cagggccata gcgctggacc agttccgaga tggcctgggt gacgaaatac gggtggcggt
     1261 atgagaacag gccactgggt cgccgcgggg cgagtcgtag cggctcgtcg tcggccctct
     1321 tggcctccgc ctgcgtgatg tacccgtcgc gggccatggc cgccagcacc agacttcgcc
     1381 gtcgcagcgc ggcgtcccgg ttcacccagg gcgagacctc ggtgggcttc cagacgatgc
     1441 ccgccagcag ggcaccctcg gccaccgaca actggcccac atccttgtcg aagtacagac
     1501 gcgaggccgc ctggatccca taggccggat gaccatagca gacctggttg aggtacatct
     1561 caaggatctc gtccttggag aactcgcgct cgatctggat cgccagcagc gcctcctgag
     1621 ccttgcggcc ataggaggcc cgggccgaga ggaagatggc acgggcaagc tgctgggtga
     1681 gggtgctggc tccggcttcg atcctcccgc tgcggtagtt ttggtacgcc acgcgaagga
     1741 tgcggggcat gtccacgccc tggtgctggt agaagcgccg atcctcaatg gcgatgtggg
     1801 cttcgcgaac atgctcggga atcgcctcga gcggcaccag ctcccggtag ccctcgtcct
     1861 ggtcgaatac cacggccagg agctgtccat ccgacgcgta aatgcggctc gccgaaggag
     1921 cccggtaccc cgcgatgtcc gtacccgagg ggagcatctt ccgcgcaccg cggtatgccc
     1981 cgtagaccgc cccggccacg ccgaccacga gcaccaacac agcaatctcg agcgcgatca
     2041 gcacacggcc agcccagtgc aacaccagac gcccggtcga cggccgggcc ggcacatggc
     2101 ttccgcgagc cgcgttgatc ctgcttgcca tggttgcctc ggccctctcc ccgcttgcac
     2161 cagcctcatt ctacaaggaa ccaccgctca ttggcgtccc ccgtgccaga gccactgatg
     2221 gcggcctacg gccggttcgc ctcgccgctg agcccgaccg gacccatggg ctctccctgc
     2281 ttcacggggg catccgaccg gaacactgac ggccgctcgg ccctcatacg cgctcggtac
     2341 cccaggtctc cggccatgca tgggccccca cccaacccga gcaccccgag cctgtcgaat
     2401 ggagcctgtc caagggcggg ccctccgcgg gcgtgactgt gccgcgactc cgtcggctcc
     2461 cggtcggcct tgccgctctc cgcccgaagt ggcatcattc gccggtcttc ggagcccctc
     2521 gggtccgacc caagtaagga gctgcccatg agccgcttgc gtgccgtgtt cctcgaccgc
     2581 gacgatacca tctcgcgggt cagccccgtg caggcccagg ccatgtaccg cgccattggc
     2641 gagatcgttg gtgtgccgga cttctcaatt accaacgatg actacacggc cctcttcacg
     2701 cgtaccatgg ccgtgcccgg catcgcgccg gtcaacacgc ccgagcgcga ggaagccttc
     2761 tggctccgct ggatgcagtt catcttcgag gaccatggcc tcaccgaagg cgcagccgag
     2821 ctctcggcgg aagtccggcg gcgcttcgtc ttccacacca tgatggagcc gttccctgag
     2881 acgcgcgaag tgctccaggc actcaaggac cggggcctga agctcggcgt catcagcgac
     2941 acctacccct ccctgaaggc atcgctcgaa tcgatgggca tcgctcacta cttggattcg
     3001 atagtggcca gcatggtggt gggcatcagc aaacctgacc cgcggatctt cgagattgcc
     3061 accaatgcac tgggcgtgaa gcctcaggag tccgtgttcg tcgacgatct gcagcggaac
     3121 gtggatggtg cccgcgccgc gggaatgacg gcattccgga tcgaccgatc ggcggcgacg
     3181 gacttctcgg gcggcgtcat cgcgtcactc cgggacctca ttgcgttcgc cgacggcgct
     3241 gcttgagctt cgggtcagcg agccaccagg tccagcgaga tgtcgagtgc gggagcgctg
     3301 tgggtgatgg ctccgcagga caccaggtcg acacccgttc gcgccacctc agcgacggtc
     3361 tcctcgcgga taccgcctga ggcctccagc agagcacgtc cggcggtcag ctccacggcc
     3421 tggcgcatca tggcgcagtc catgttgtcg agcatgatga catcggcgcc agcctcaaga
     3481 gcctcacgga gctggctcag gctctgcacc tcgacctcga cacgcatggt gtgcgggcac
     3541 agatctctgg cagcccgcac ggccggcccg atgcccccag cggctgcgat gtggttgtcc
     3601 ttgatgagca ccgcatcggc gagatggaag cggtgattgc tcccgcctcc aacacgcacg
     3661 gcatacttct ggagcacgcg gaggcccggg actgtcttcc gagtatcgac gagccgcgcg
     3721 cgcgtgccct cgatcaggag ccgcatcgtc cgtgtggccg tggcgacacc agagagctgc
     3781 tgcaggaagt taagtgccgt gcgctcggcc gtgaggatcg atcgggcacg gccggaaacc
     3841 tcggccagcc ggtcgcccgg ctcgacgagg tctccatcgg cggccaccgc cgcgaaaccg
     3901 agcgacggat ccacgcgcag gaacacggcc tcagcgatgc ccaggcccgc aacgacgccg
     3961 cgctgtttgg cctgaatgac cgcatcggca tagacggtgg cggggaccgt ggccatggtg
     4021 gttatgtcgc catgggcgac gtcttcctcg agagccaggt caatcaggcg gcggacggcc
     4081 ggcaggccgg caatgtcgtt cacggctcag accagctcca gcatccgatc gatggcccgg
     4141 tgggcgcggc gggcgatgtc gtcggggacc gtgatacgcg gcgccagcgt ctcgagagcc
     4201 gagaggacgt tctccagcgt gttcatcttc atgttcgggc acacggccac atacgagggg
     4261 cagtagaagc gcttgcccgg gttctctcgc tccagccggt agatgagccc gacctcggtg
     4321 gcgatgatga actcctgtgc gtcg
//
LOCUS       DAOSPL010000163         4340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20293_length_4340_cov_3.650875, whole genome shotgun sequence.
ACCESSION   DAOSPL010000163 DAOSPL010000000
VERSION     DAOSPL010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20293_length_4340_cov_3.650875"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PLQ54_04270"
     CDS             complement(<1..304)
                     /locus_tag="PLQ54_04270"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HOS92503.1"
                     /translation="MIDTQPGAPRSVFTSELVELHDTVLRMGTFVMSMLERGLQALVE
                     QNLKLAEEVRRSDDVADALDIGIEEAALRLLALQHPMASDLRCVAGAIRIAADLERV"
     gene            complement(301..1116)
                     /gene="pstB"
                     /locus_tag="PLQ54_04275"
     CDS             complement(301..1116)
                     /gene="pstB"
                     /locus_tag="PLQ54_04275"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOS92504.1"
                     /translation="MATAPGLMVGSVDADPVSDCRCAKFETHGLTVHYGGFEALKAVD
                     VAIGSCEVTALIGPSGCGKSTFLRCLNRMNDMIDGVRISGQVVMDGQDVYDRRVDVVE
                     LRKRVGMVFQQPNPFPMSIFDNVAYGPRLHGTRKMSDLEVIVERTLREAALWDEVKDK
                     LRQSALALSGGQQQRLCIARVMAVQPEVILMDEPCSALDPNATFRIEELMLSLRENYT
                     VIIVTHNMYQAARCSQKTGFFLLGELVEFGDTEQVFQAPADRRTEDYIRGRFG"
     gene            complement(1123..2073)
                     /gene="pstA"
                     /locus_tag="PLQ54_04280"
     CDS             complement(1123..2073)
                     /gene="pstA"
                     /locus_tag="PLQ54_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOS92505.1"
                     /translation="MSTSTLPRAEAWPSTPSARRARRVQAVAFVALGASALAIMVPAV
                     LFVGFVVVRGASAINWEFITAMPRDNMRAGGIMPAIVGSLYLVVVTMALATPVGILAA
                     VYLSEYARKGPLVRAIRVAILNLAGVPSIVYGLFGMGLFVMVVMPQVLRLTRGGGESG
                     KSACLLAAAATMAVLILPIVIATTEEALRQVPQGLRAASLALGATRWQTIWRVVIPNA
                     LPGILTGLVLAVGRAAGETAPILLTGAFFWMSHLPRPAEALLEPFMALPFHLFTVSTQ
                     MPDAPEKLRWGTALVLIIMVVGANSIGIVIRSWLRTRRKV"
     gene            complement(2063..3010)
                     /gene="pstC"
                     /locus_tag="PLQ54_04285"
     CDS             complement(2063..3010)
                     /gene="pstC"
                     /locus_tag="PLQ54_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOS92506.1"
                     /translation="MKLRRIGRVLEWAIELVIRSCGVASVVFVILILAFLLRNALPAF
                     NSVSLGEALGGRLWRPTSNPPQFGFLPLILGSALVTVGSLLISVPIGIGAAVFLAEVA
                     PRRVREIAKPIVELLGAVPSVVFGFVGLMLVGAWIQRASRAAGDALPGPDWLKTALNM
                     PSSMAAITGAVLLALMTLPTIVSIAEDALDSVPERFRQGSLALGATRWQTIRGVTIPA
                     ARSGIMASVMLGVGRAVGETMTVLMVTGNAAVVPSLTTGLFRPVRTMTATIAAEMGET
                     AHQTPHFHVLFLLGLLLFLITFCVNTTADIITHRGRHEH"
     gene            complement(3089..4018)
                     /locus_tag="PLQ54_04290"
     CDS             complement(3089..4018)
                     /locus_tag="PLQ54_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOS92507.1"
                     /translation="MRRSQWTSIAISLGLVGSALLGGCAKPEETEEPAAPVVSTKKAS
                     QPGAGGAGGEIVAKGSDTLLQVAQAMAEAYQAANPGVKISVSGGGSGTGFQALIEKTC
                     DIANASRQIKNEEVEQAKAKGIEPVENLIGYDGIAVIVNKANPVNELTVDQISDLYVG
                     STNSWDKLGGKGEVVLVSRDSTSGTYEYFLEEIIKKGDKKSTREFAPSIMALQSNPDV
                     RRQVTESEGAIGYIGLGYVDDSVKVIGVIGKKGGAAVMPSVETVKDGSYPISRPLYMY
                     TDKNAPKKVSDYISWVLGDEGQAIVKEKGFVPK"
     gene            complement(4137..>4340)
                     /locus_tag="PLQ54_04295"
     CDS             complement(4137..>4340)
                     /locus_tag="PLQ54_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS92508.1"
                     /translation="KSRERVFERFYRIDKGRSRLVGGTGLGLSIVRHAIERNGGRVWV
                     ESPEHPGSRFVVLLPARPPATPP"
BASE COUNT          801 a         1440 c         1371 g          728 t
ORIGIN      
        1 ccacgcgctc gagatcggcg gcgatgcgaa tggcgccggc cacgcagcgc agatcactgg
       61 ccatggggtg ctggagcgcg aggagtctca gcgccgcctc ctcgatgccg atatcgaggg
      121 catcggccac gtcgtcgctt cggcgcactt cctccgccag cttcaggttc tgttcgacca
      181 gggcctgcaa gccgcgttcg agcatgctca tcacaaaggt gcccatgcga agcaccgtgt
      241 cgtggagctc gaccagctcg gacgtgaaga ccgatcgtgg cgcgcctggc tgagtgtcga
      301 tcatccgaac cgccccctga tgtagtcctc ggtgcgccgg tcggccggtg cctgaaagac
      361 ctgctcggta tcaccaaact caaccagctc gccaaggagg aagaacccgg tcttctgcga
      421 gcagcgcgcc gcctggtaca tattgtgggt cacgatgatg acggtgtagt tctcccgcag
      481 ggacagcatg agctcctcga tgcggaatgt ggcgttaggg tccagggcgc tgcacggctc
      541 atccatgagg atgacctcgg gctgtacggc catgacccgt gcgatacaca ggcgctgctg
      601 ctggccgccg gagagcgcga gcgccgattg gcgcagcttg tccttcacct catcccacag
      661 ggccgcctcg cgcagagtgc gctcgacaat cacctcgagg tccgacatct tgcgtgtccc
      721 gtggagccgc gggccatagg ccacgttgtc gaagatgctc atgggaaagg gattgggctg
      781 ctggaacacc atcccgaccc gtttccgcag ttcgaccacg tcgacacgtc ggtcgtagac
      841 atcctgacca tccatgacga cctgaccgct gatgcggaca ccatcaatca tgtcgttcat
      901 gcgattgagg caccgcagga aggttgactt cccgcatccc gatgggccga tgagcgccgt
      961 gacctcacag cttccgatgg cgacgtccac cgctttgagg gcctcgaatc ccccgtagtg
     1021 cacggtcaac ccgtgggtct caaacttggc gcaccggcag tcactgacgg gatccgcatc
     1081 gacactgccg accatgagtc cgggtgccgt agccatccga gcctagacct tccttctcgt
     1141 ccgcagccag ctccggatca cgatcccgat ggagttggct ccgacgacca tgatgatgag
     1201 cacgagggcg gtgccccagc gaagcttctc cggggcatcg ggcatttgcg tcgaaactgt
     1261 gaagagatgg aacgggagcg ccatgaaggg ttcgagcagt gcctcggctg gtcggggcag
     1321 gtgggacatc cagaagaagg cacctgtcag caggatcggc gccgtctcgc ccgccgcccg
     1381 ccccaccgcg agcaccagcc cagtcaggat gccgggcaag gcgttaggga tcacgacacg
     1441 ccagatcgtc tgccaccgag tggctcccag ggcgaggctg gcggcgcgga gcccctgggg
     1501 gacctgccga agcgcctctt ccgtggttgc gatgacgatg ggcaggatca gtaccgccat
     1561 ggtcgcggca gcggccagaa ggcacgcact cttgccggac tcgccaccgc cccgtgtcag
     1621 ccgcagcacc tgcggcatca cgaccatcac gaagagaccc atgccgaaga ggccatatac
     1681 gatggagggc acacccgcca ggttgagaat ggcgacgcgg atggcgcgga ccaggggccc
     1741 ctttcgcgca tactcgctca gatagacagc ggcgagtatg ccaacgggag tggccagggc
     1801 catggtgacc acgaccaggt agagggatcc cactatggcg ggcatgatgc cgcccgcccg
     1861 catgttgtcg cgcggcatgg ccgtgatgaa ctcccagttg atggccgaag ccccgcgcac
     1921 gaccacaaag cccacgaaca gcacggccgg taccatgatg gccagtgccg aggcgcccag
     1981 cgccacgaag gcgacggcct ggacccggcg ggcccgccgg gccgacgggg tactgggcca
     2041 ggcctccgct cgcgggagcg tgctagtgct catggcgccc cctgtgcgtg atgatgtcag
     2101 ccgtcgtgtt cacgcaaaag gtgatgagga agagcagcag tccgagcagg aagaggacgt
     2161 ggaagtgagg cgtctgatgg gcggtctccc ccatctcggc agcgatggtg gccgtcatgg
     2221 ttcgcacggg acggaacagg ccggtggtca atgacgggac caccgcggcg ttgcccgtga
     2281 ccatcagaac cgtcatggtc tcgccgaccg ccctgcccac tccgagcatg acggaggcca
     2341 tgatgcccga acgcgcggcc gggatggtga cgccccgaat cgtctgccag cgggtggcgc
     2401 cgagagcaag agagccctgc cggaaacgct ccggcacgct atccaacgca tcctcggcga
     2461 tcgacacgat ggtgggcaga gtcatgagtg ccaacagcac cgcgcccgtg atggccgcca
     2521 tgctcgaggg catgttcagc gcggtcttga gccaatcggg acccggcaac gcatcccccg
     2581 ctgccctcga tgctctctga atccacgcgc cgactagcat cagaccgacg aagccgaaca
     2641 cgacagatgg gactgcgccc agaagctcca cgatgggctt ggcgatctcg cgcacgcgcc
     2701 gaggcgccac ttcggccagg aacacggcag ccccgatccc gatgggaacc gagatcagca
     2761 acgagccgac cgtgacgaga gcactgccca gaatcagcgg caggaaccca aactgaggag
     2821 ggttgctcgt cggccgccac agtctgccgc ccagcgcttc acccaggctc acgctgttga
     2881 atgccggaag cgcgtttcgc agcaggaacg ccaggatcag gatcacgaac acaacggagg
     2941 ccacgccaca ggagcggatg acaagctcaa tggcccactc caacacgcgg ccaatgcggc
     3001 gcagcttcat cgggtgtcct ctgcatcgga gatggcggcc gctgccggcc gccatctccg
     3061 tcatgggtgt gcgagctctg tgcagggcct acttgggcac gaagcccttc tctttgacga
     3121 tggcttgtcc ctcatcgccc aagacccagc tgatgtagtc cgagaccttc ttaggcgcgt
     3181 tcttgtcggt gtacatgtaa agcgggcgtg agatggggta ggagccatcc ttcacggtct
     3241 cgacggacgg catcacggcc gcgccgccct tcttgccgat cacgccgatg actttcaccg
     3301 aatcgtcgac atacccgagg ccgatatagc caatggcgcc ctcagactcg gtcacctgac
     3361 gccgaacgtc agggttggac tgcagggcca tgatgctcgg ggcgaactcg cgcgtgctct
     3421 tcttgtcgcc cttcttgatg atctcctcaa ggaagtactc ataggttcca gaggtggaat
     3481 cgcgggagac caggacgacc tcgcccttgc caccgagctt gtcccacgag ttggtcgagc
     3541 caacatacag gtcggagatc tggtccacgg tgagctcgtt caccggattc gccttgttga
     3601 cgatgacggc gatgccgtcg tagccaatca ggttctcgac cggctcgatt cccttggctt
     3661 tggcctgctc aacctcttca ttcttgatct gacgcgaggc gttggcaatg tcgcacgtct
     3721 tctcgatcag cgcctgaaag ccggtgccag aacccccacc ggacacgctg atcttgacgc
     3781 ccgggttggc cgcctgatag gcctcggcca tggcctgagc gacctgcaac agggtatcgg
     3841 accccttggc cacgatctcg cccccggcgc cgccggcccc cggctgagat gcctttttag
     3901 tgctcaccac gggcgctgcc ggctcctcgg tctcctccgg cttggcgcat cccccaagca
     3961 gtgcgctgcc gacaagcccc agggatatcg cgatgctggt ccactgactt cgtctcatct
     4021 gggtctggct cccgccttat cgtgttgtgt ggccccgctc ggccttcagg agatgtcggc
     4081 cagcgagcct taacacattc tcggggcacg gcgcgtccga ggggcttaag cccgtgttaa
     4141 ggcggcgtcg ccggaggtcg ggcgggcagc aacaccacga accgcgaacc cgggtgctcg
     4201 gggctctcga cccacacgcg gcccccgttg cgctcgatgg cgtggcggac gatactcagc
     4261 ccgaggcccg tgccacccac cagacgagac cgccccttgt cgattcggta gaaacgctcg
     4321 aagacgcgct ctcggctctt
//
LOCUS       DAOSPL010000164         4338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20304_length_4338_cov_2.774224, whole genome shotgun sequence.
ACCESSION   DAOSPL010000164 DAOSPL010000000
VERSION     DAOSPL010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20304_length_4338_cov_2.774224"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2409
                     /locus_tag="PLQ54_04300"
     CDS             <1..2409
                     /locus_tag="PLQ54_04300"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92509.1"
                     /translation="VLASLRDASDEFDRRREAVTEELRALKTRLEEAATEATRAREQQ
                     QAVRDELGGLEQERQAAMERQRSASEALEARRRRGTELSEQLRSLESRLATLRELRDS
                     YHGIAHGAQSLLEARESGALQGIIGTVAERVVVEHPYQAAVAAALGDRAQYVIVADRS
                     AATSALEYLRATSGGRATLLPLDQIGAADPPSSPGPSMALRSAVTVCRCDDAVRPALQ
                     HLLGRVFIAESRDAALQALSTSRAPIAVCTPEGDCYFADGSVTGGSEESAAEDPLLRT
                     TEIAELEARVAEITALREQARREQEAAEVERREAEALLREIQSRSEALRSQQGRIAAE
                     LARLGAAAEALQRDDVRLTREVDHLATRQAEVSERIAALEAELAQAPQVAAPDADEVN
                     RELAALAAQRDDWAAQVSDNRAELATARERLESRKAELARLAEEMAHASSRAELLERE
                     IRNLDESAAENQRVVGEQDGHIAELQNRANELHQALEERNQVRADLMAQLQRLSEVLR
                     EARAAAPELQQRLQVAEVRRARNEATLATIVEHLATEYEMTVEEAARQAPPLENERRA
                     SEDARRLRVRLKELGDVNLGAIAEYDRLTQRRDFLSTQKADLDAARDNLRAIIAEIDE
                     ATRAQFMDTFNAVNAAFGEIFTKLFEEDEARANGRPRGERGVGHAELVLTDPQNLLET
                     GVEVIAQVPGKRVQNLLSLSGGERALTAISLLFAMLKVKPPPFCVLDEIDAPLDEVNT
                     RRFADLLRELSGQTQFLVITHARGTMEACDVLYGVTMQEQGVSLSLSVTLSQAETVAE
                     TGKT"
     gene            complement(2434..3885)
                     /locus_tag="PLQ54_04305"
     CDS             complement(2434..3885)
                     /locus_tag="PLQ54_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92510.1"
                     /translation="MDTMVRRILILSLLPSMPSTALSAPATAERTLDNGLHAIAQQTR
                     SSEVFCIDVVVRSDPSEDGDLPGLRCIAAGTMLAGAEGWASERTQALLQGIDEQDGST
                     GGRATDEFVEVHISGPASFLDEALAFMIAALKSGAYPEDAVKAQAQAQAVFIDHSAKE
                     PNLEHMVRRVLYPVPQVEELLGTPESLSAITRERVADLARRLFAPGRVFCAIVAPRTP
                     GEILDAVAGLCADWPGGEPAPALPDWREAVRQREQDASSALLPLPYRSQVIEAGTAPV
                     AQIMFGFEVPPVAAPDWPLADLTAALLDPAASGRLSTEPVFERSDASAGPGAAGVACS
                     SARAVAYNHMVAFAYARGPVGPQITELDWYRILSTQATQVDESLRHAIRSLAGEPPSK
                     AEMERARRLVLGSNRMRLASARDRAQYLALMRLAGVEGDPLEWYETAIRQATADDVVH
                     FAATYLTLDKLAMAVLLPAQSQAPGFTGFPQLQ"
     gene            complement(3915..>4338)
                     /locus_tag="PLQ54_04310"
     CDS             complement(3915..>4338)
                     /locus_tag="PLQ54_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92511.1"
                     /translation="RFQRRIVGERKLADEMLTIYLTQRDPGAFYVWAVTPADGADAAV
                     SAVLEEIADIRAKGLPAEELDRAKRQILGSFAVQSETYMDQAATLGFYESIGGFEFAL
                     EYEDRVSAVTSADIQRVASTYLDPELYTLVQVRPKAAH"
BASE COUNT          730 a         1440 c         1506 g          662 t
ORIGIN      
        1 gtgctggcct cgctgcgaga tgcctcggac gagttcgacc ggcgccgtga agccgtcact
       61 gaggagctcc gcgcgctgaa aacccgactc gaggaggcgg ccaccgaggc cacgcgggcc
      121 cgagagcagc agcaggcagt gcgcgatgag ctgggtggcc ttgagcagga acgccaggcc
      181 gccatggagc gccaacggag cgccagcgag gccctggagg cgaggcggcg gcgtgggacc
      241 gagctgtccg agcaactgcg gtccctggaa tcgcggttgg ccaccctgcg tgagctgcgc
      301 gacagctacc acggcatcgc gcacggtgcc cagagcctgc ttgaggcgcg ggagtccggc
      361 gcgcttcagg ggatcattgg cacggtcgcg gagcgagtgg ttgtcgagca cccctatcaa
      421 gccgctgtcg cagcggccct gggtgatcgg gcgcagtatg tcatcgtcgc cgaccggtct
      481 gcggccacat cggctctgga gtacttgcgg gccacatcgg gcggcagagc cacgctgctc
      541 ccattggacc agatcggtgc cgccgatccg ccgtcgtcgc cgggtccttc catggcgttg
      601 cgctctgcgg tcacggtatg ccggtgcgac gatgcggtga ggccggcact gcagcacctg
      661 ctcggccggg tgttcattgc ggagagccgc gatgccgcgc tccaggccct ctcgacatcc
      721 cgagcaccca tcgccgtctg cacgcccgaa ggcgactgct atttcgctga cggctccgtc
      781 accggcggga gcgaggagtc ggctgccgag gacccactcc tccgtaccac tgagatcgca
      841 gagctcgaag cgcgcgttgc ggagatcacg gccctccggg agcaggcacg gcgagagcag
      901 gaggctgccg aagtcgagag acgagaggcc gaggccctgc tgcgcgagat ccagtcgcgg
      961 tccgaggcgc tccgaagcca gcagggccgc atcgcggcgg agctggcgcg cctgggggcc
     1021 gccgctgagg cacttcagcg cgacgacgtt cggttgacgc gggaagtcga ccaccttgcg
     1081 acacgccaag ccgaggtctc tgagcgcatc gccgcactcg aggccgagtt ggcccaggcg
     1141 ccgcaggtgg cggcgcctga tgccgacgag gtcaaccggg aattggctgc cctggcggcc
     1201 cagcgggacg attgggcggc gcaggtctcg gacaaccggg ccgaactggc cacggcgaga
     1261 gagcggctcg agtcgcgtaa ggccgagctc gcgcggctcg cggaggagat ggctcatgcc
     1321 tccagccgag ccgaactact cgagcgcgag atccgtaacc tcgacgagtc agcggccgag
     1381 aaccagcggg tcgtcgggga gcaggatggg cacatcgccg agctccagaa ccgggcgaac
     1441 gagctccacc aggcgctcga ggaacgcaat caggtgcgcg cggatctgat ggcccagctg
     1501 cagaggttgt cggaggtgct ccgcgaggcg cgcgcggcgg cacccgagct ccagcagcgc
     1561 ctccaggtag ccgaggtgag gcgggcgcgt aacgaggcga cactggcgac catcgtggag
     1621 catctggcca cggagtatga gatgacggtc gaggaggccg ccagacaggc cccgcctctc
     1681 gagaatgagc ggcgtgcgtc ggaggacgca cgccggcttc gagtccgtct gaaagagctg
     1741 ggcgacgtga acctcggcgc catcgccgag tacgatcgcc tcacccagcg gcgggacttc
     1801 ctgtccacgc agaaggccga tctggacgcg gcgcgcgaca atctgagagc gatcatcgcc
     1861 gagatcgacg aggccactcg tgcccagttc atggacacgt tcaatgctgt gaatgcagcg
     1921 tttggtgaga ttttcaccaa gctcttcgag gaagatgagg cccgggccaa tggccgacct
     1981 cgcggcgaac gtggagtggg ccatgccgag ctggtgctca ccgacccgca gaacctgctc
     2041 gagaccggcg ttgaggtcat cgctcaggtc cccggcaagc gcgttcagaa ccttctgtcg
     2101 ctctccgggg gcgaaagggc cctgacggcc atctcactgt tgttcgccat gctcaaggtc
     2161 aagccgccgc ccttctgcgt gcttgacgag atcgatgccc cgctggacga ggtgaacacg
     2221 cgccggttcg ctgacctgct gcgcgagctc tcgggacaaa cgcagttcct ggtcattact
     2281 catgcccgcg gcaccatgga agcatgcgac gtgctctacg gtgtgacgat gcaggagcag
     2341 ggcgtatccc tatcgctctc ggtgaccctg agccaagccg agaccgtggc cgagacgggg
     2401 aagacctagg ccggcgtttg gcgcccctgc gccctactga agctgtggga atcctgtgaa
     2461 gcctggagcc tgcgactggg ccggcagcag gaccgccatg gccagcttgt ccagtgtcag
     2521 gtacgtcgcc gcgaaatgga cgacgtcgtc cgccgtggcc tgccggatgg ccgtctcata
     2581 ccactcgagg gggtcaccct cgacaccggc caggcgcata agggctaggt actgtgcgcg
     2641 gtcgcgggcc gacgcgagcc gcatccggtt cgacccgagg acgagccgcc gagcgcgctc
     2701 catttccgcc ttcgatggag gctcgcccgc cagcgaccgg atggcgtgcc gcagcgactc
     2761 atctacctgc gtggcttggg tcgacagaat ccggtaccag tccagctccg tgatctgggg
     2821 ccccaccggt cctcgggcat aggcaaaggc caccatgtgg ttgtaggcca cagcacgggc
     2881 cgaggagcaa gcgacccctg ctgcaccggg ccccgccgag gcatcggacc gttcgaagac
     2941 cggctcggtc gacagccggc cgctggccgc ggggtcaaga agcgccgcgg tgaggtcagc
     3001 cagcggccag tcgggggccg ccaccggcgg tacctcgaaa ccgaacatga tctgggcgac
     3061 cggcgcggta ccggcctcga tgacctggct gcggtagggc agcggcaaca gcgcactgct
     3121 cgcgtcctgc tctcgttggc gaaccgcctc tcgccagtcc ggaagtgctg gggccggctc
     3181 accccccggc cagtctgcgc agagccccgc cacggcgtcg aggatctcac cgggcgttcg
     3241 gggcgccacg atcgcgcaga aaacccggcc aggggcaaac agccgccgcg ccaggtcggc
     3301 cacccgctcc cgggtgatgg cgctcaggct ctcgggcgtg cccagcaact cctcgacctg
     3361 cggcaccggg tacaggacac ggcgaaccat atgctcgagg ttcggctcct tggcgctatg
     3421 gtcgatgaac accgcctggg cttgagcttg agctttcacg gcgtcctcgg gatacgctcc
     3481 gctcttgagg gcggctatca tgaaggcgag ggcctcgtcg agaaaggagg cgggacccga
     3541 gatgtggacc tcgacgaact cgtcggtggc tctaccgccc gtggagccgt cctgctcgtc
     3601 gatgccctga agaagcgcct gagtccgctc ggaggcccag ccctcggctc cggccagcat
     3661 cgtcccggcg gcgatgcatc gcagtccggg gagatcgcca tcctcgcttg gatctgaccg
     3721 gaccacgaca tcgatgcaga acacctcgga gcttcgtgtc tgctgcgcga tggcatgtag
     3781 gccgttgtcc agcgtgcgct cagccgtcgc gggtgccgac agggccgtcg agggcatgct
     3841 cggcagtagg ctcagtatca agatccgtcg aaccatcgtg tccacgggga gccgcctctc
     3901 tgggggcctg ggcgctagtg tgccgccttc gggcgcacct gaacgagcgt gtacagctcc
     3961 ggatccaggt acgtgctggc cacccgctgt atgtccgctg aggtgaccgc gctgactcgg
     4021 tcctcgtatt ccagtgcgaa ctcgaagcca ccgatcgatt cgtagaaccc gagcgttgcg
     4081 gcctgatcca tgtacgtctc agactgcacg gcgaagctac cgagtatttg tcgcttggcg
     4141 cgatcgagtt cttccgccgg aaggcctttc gcgcggatgt cggcaatctc ctcgagcacc
     4201 gcgctcaccg ccgcgtcggc cccgtcggcg ggagtaacgg cccagacgta gaaagcacca
     4261 gggtcgcgct gggtgagata gatggtcagc atctcatcag ccagcttgcg ctcgcccacg
     4321 atccgcctct ggaaccgc
//
LOCUS       DAOSPL010000165         4331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20350_length_4331_cov_2.960711, whole genome shotgun sequence.
ACCESSION   DAOSPL010000165 DAOSPL010000000
VERSION     DAOSPL010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20350_length_4331_cov_2.960711"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PLQ54_04315"
     CDS             complement(<1..332)
                     /locus_tag="PLQ54_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92512.1"
                     /translation="MSNWTGVACACLWCAGHSYAQQEPSFRPPAMWEYSAPLIAPEER
                     DRDPSRAQKDPTVVFFEGRWHVFMTVKLPGRSAIEYCSFADWAQADASPRTILRVSDS
                     DYYCAPQV"
     gene            complement(381..1442)
                     /locus_tag="PLQ54_04320"
     CDS             complement(381..1442)
                     /locus_tag="PLQ54_04320"
                     /inference="COORDINATES: protein motif:HMM:NF015617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-reducing end alpha-L-arabinofuranosidase
                     family hydrolase"
                     /protein_id="HOS92513.1"
                     /translation="MRRSVRAILGVALVGSVLVDPPCAPQIQETEAASSGPGVDNLLA
                     GRFAWRAGPPLISCPESTTDTYIAIKDPSVVFYQGRWHLFCSVIREGAPYQTEYLSFP
                     DWAHTAKAARHILPVHRDGRYGAPQVFYFEPQGKWYLICQAMHDTWDPQYAASCATTS
                     DIGDPDSWSQLRPMGALPADGKPGLDFWVICDDRAAHLFFTTLDGRMWREETSLDQFP
                     GGWSQPRLALQGDIFEAGHIYRLRGIGRYLALIEAQSGHGWRYYKAYLADRLDGAWEP
                     LAATADAAFASMANTEHSGERWTDCISHGELLRAGYDQHLEVDPAGMRFLFQGALDRE
                     TEGRGYGQITWRLGLLEAR"
     gene            1656..2720
                     /locus_tag="PLQ54_04325"
     CDS             1656..2720
                     /locus_tag="PLQ54_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOS92514.1"
                     /translation="MRPTRREFLQRTGMAAVTAALGMPAPARAEGETMAEHRCDWMVE
                     EGPYGMMVHWIAPGPAPEQGEWIQDLDKAVDGFRIDSFLEQFEASGAAWLIFTIGQNS
                     SRYASPNPVLDRLAGPGHCSKRDLVLELAQGVHRLGKRFIAYLPAEVNAPEDLHEPFG
                     WNPADQTTFQQRYTDFIREYSVRFGELCDGWWFDGCYTWDVFHNSLYDWPLWCGAARA
                     GNPQAALAFNDGSFCIGITKPLTTEQDYLSGEVELLVGGRIRLGRDAGSPLILPTSRF
                     AEGTRCQWHALVPIDCFWGHHTPGPMEPPKYADDDLLVLLGACQRTQAVATLNVGIYQ
                     EGHLGSATVAQLARLRKRLG"
     gene            complement(3006..4181)
                     /locus_tag="PLQ54_04330"
     CDS             complement(3006..4181)
                     /locus_tag="PLQ54_04330"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92515.1"
                     /translation="MTTTAEAAPRRITIIGAGSTGRGHLGELAFEAGWDLVFVDKDFD
                     LVSRLTRAGQYTVDLYGPAHHRTVTVDRLRAYHTSDIDGIIAASLDVPLVLTAVFSHN
                     LHQVAPLIAALIGARAGQGIATPLNVVCCENMQDSSSMLRAQVMPQLSPAQAAYAEAQ
                     VGFPDCMISRVVPLPDEDPLRLVAEDYNEWTVDAHQFVGPAVALPCMELVDNQAARLA
                     RKFFMHNGAHAVCGYMGFHRGHTYIHQAVADAVVLEWVVGAIEELAPIVARRYGLDAG
                     SVRDYGLELGARGAIAPMRDLILRVVRDPLRKLSRDERLTAPAVMALEYGLPCEHLVQ
                     TIAAVLHYHHKDDPQSLVMRARLEAEGPAAVSGFLGLPADHRLVRLVQDAYAAWRGV"
BASE COUNT          757 a         1486 c         1437 g          651 t
ORIGIN      
        1 aagacctgcg gggcgcagta gtagtcgcta tcgctcaccc tcaggatcgt gcgcggcgag
       61 gcgtcggcct gcgcccagtc cgcgaaagag cagtattcga tcgccgaccg gccgggcagc
      121 ttcaccgtca tgaagacatg ccacctgccc tcgaagaaga cgacggtcgg atccttctgc
      181 gcccggctgg gatctcggtc gcgctcctcc ggagcgatga gcggggcgct gtactcccac
      241 atggccggcg gcctgaaact gggttcctgc tgagcgtacg agtggccggc gcaccacaga
      301 cacgcacagg cgacgcccgt ccagttcgac atggagagcc tccgttcccc gccgaaaccc
      361 gacggctgag cgccccgtcc tcaacgagcc tccagcagcc cgagccgcca ggtgatctga
      421 ccgtatccgc gcccctcggt ctcgcggtcc agcgcgccct ggaacaggaa gcgcattccg
      481 gcagggtcta cctcgagatg ctggtcatag ccggcgcgaa gaagctcccc gtggctgatg
      541 cagtcggtcc agcgctcgcc agaatgctcg gtgttagcca tgctcgcaaa cgccgcgtcc
      601 gccgtggcag ccagaggctc ccatgcacca tccagccggt cagccagata ggccttgtag
      661 taccgccagc catgcccgga ctgggcctcg atcagcgcaa ggtacctgcc tattccccgg
      721 agccgataga tgtgccccgc ctcgaagatg tcgccctgca gggcgagcct gggttgggac
      781 caccctccag ggaactggtc cagactggtc tcctcgcgcc acatgcgccc gtccagcgtc
      841 gtgaagaaca gatgtgccgc ccggtcgtcg cagatgaccc agaagtcgag cccgggcttg
      901 ccgtcggccg gaagcgcgcc catgggtctg agttggctcc acgaatcggg gtccccgatg
      961 tcactcgtgg tggcacagga tgccgcatac tgagggtccc acgtgtcgtg catcgcctgg
     1021 cagatcaggt accacttgcc ctgcggctcg aagtagaaca cctggggcgc accgtaccgg
     1081 ccatccctat gaaccgggag tatgtgccgc gccgccttcg ccgtatgggc ccagtcaggg
     1141 aacgacaggt actcggtctg atacggcgcc ccttcgcgga tgaccgagca gaacaggtgc
     1201 catcggccct ggtagaagac caccgacggg tctttgatcg cgatgtacgt atcggtggtc
     1261 gactcgggac agctgatcag gggcggcccc gcgcgccagg cgaaccgccc ggcgagcagg
     1321 ttgtcgaccc cggggccact cgaggccgcc tccgtctcct ggatctgggg ggcacagggc
     1381 gggtccacga gcacagaccc caccagggcc actcccagaa tcgcgcgcac cgaacgcctc
     1441 atgaccgacc tccggctcct cccgctctgg acgcggcacg gcgatgactc aagcccgcta
     1501 cccacagcct tatggttcca gacgccgggt tgacacccgg tgtctcgcct gtactactct
     1561 gagcggtgca caccgagcgg agtacgccat cagcggtcag gaagtcctcg cacccatcgc
     1621 gacgaaccac ctgaccgagg gagatgcgcc tgaacatgag accaacgcgg cgtgagttcc
     1681 tgcagaggac aggcatggcg gcggtcacgg cagccctggg gatgccggcc ccggcgagag
     1741 cggaagggga gaccatggcg gagcatcggt gcgactggat ggtcgaagag ggcccgtacg
     1801 ggatgatggt ccactggatc gcgcccgggc ccgcgcccga acagggcgag tggatccagg
     1861 acctggacaa ggcggtcgac ggcttccgga tcgacagctt tctcgagcag ttcgaggcca
     1921 gcggcgccgc gtggctcatc ttcaccatcg gacagaactc gtcgcgctat gcgagcccca
     1981 acccggtact cgaccggctg gctggccccg ggcactgctc gaaacgcgac ctggtgctgg
     2041 agctggccca aggcgtccac cgactcggca agcgcttcat tgcgtacctg ccggcagagg
     2101 tgaacgcgcc cgaggacctg cacgaaccct tcggctggaa ccccgccgac cagaccacct
     2161 tccagcagcg ctacacggac ttcatccgcg agtactcggt gcggttcggt gagctctgtg
     2221 acggctggtg gttcgacggc tgctacacgt gggatgtgtt ccacaacagc ctctacgact
     2281 ggccgctgtg gtgcggcgca gcccgggcgg ggaacccgca agccgcgctg gctttcaacg
     2341 acggctcttt ctgcatcggc atcacgaagc ccctgacgac cgaacaggac tacctatcgg
     2401 gcgaggtgga gctgctggtc ggtggcagga ttcgactcgg tcgcgacgcg ggctccccgc
     2461 tgatcctgcc cacaagccgg ttcgccgagg gcacgaggtg ccagtggcat gcgctggtac
     2521 cgatcgactg cttctggggc caccacaccc ccggcccgat ggagccaccc aagtacgcgg
     2581 acgacgatct actcgttctg ctcggtgcgt gccagcggac tcaggcggtc gcgacactga
     2641 acgtcggcat ctaccaggag gggcatctcg gcagcgcaac ggtggctcag ctggcgcgtc
     2701 tgcgaaagcg gctggggtag cggacggggg ctctgcagac gcaagccctt tccccggccc
     2761 atgggcggtg ctccaggccc gggccgtgcg caaaaggccc gaacaccgca ccgcccggcc
     2821 tcgaggaggg gccgcacagg gatgcgcccc aggggcgggt tgggggacca cacacaacgc
     2881 ccgggtgttc gcccccatgg cgcccctccg agccaggccc atgggccgct ggagccctcg
     2941 gttaccgccc acacgtcccc ggccctcgcc gcgaacggac ggggccaaga ccaacccccg
     3001 ccgtcctaca ctccccgcca cgccgcatac gcatcctgca ccagccgtac gagccgatgg
     3061 tccgccggca ggccgaggaa gccggagacg gccgcggggc cttcggcctc gagccgggca
     3121 cgcatgacca gcgactgcgg gtcgtccttg tggtgatagt gcagcacagc ggcgatggtc
     3181 tggaccaggt gctcacacgg gagcccgtac tcgagggcca tcacggcggg cgccgtgagg
     3241 cgctcatcgc gtgaaagctt gcgcaacgga tcgcggacga cgcggaggat gaggtcgcgc
     3301 atcggcgcga tcgcgccgcg agcacccagc tcgaggccgt aatcgcgcac ggacccggcg
     3361 tcgagcccat accgtcgcgc cacaatcggg gcgagttcct cgatggcgcc aacgacccac
     3421 tcgagcacta cggcatcggc cacggcctgg tgaatgtagg tgtgtccccg gtggaatccc
     3481 atgtagccgc agacggcgtg cgcgccgttg tgcatgaaga acttccgcgc aagccgcgcc
     3541 gcctggttgt cgacgagctc catgcatggc aaggccacgg cggggcccac gaactggtgg
     3601 gcatccaccg tccactcgtt gtagtcctcg gccacgagac ggagggggtc ctcgtcgggg
     3661 agcggcacca cgcgcgagat catgcagtcg gggaaaccaa cctgcgcctc cgcgtacgcg
     3721 gcctgcgccg ggctcagttg cggcatgacc tgtgctcgga gcatggatga gctgtcctgc
     3781 atgttctcgc aacagaccac attcaggggc gtggcaatgc cctgtccggc tctcgcaccg
     3841 atgagcgcgg cgatcaacgg ggcaacctgg tgcaggttat gcgagaacac cgcggtcagc
     3901 accaggggca catcgaggct ggcggcgatg atgccatcga tgtcggacgt gtggtaggcg
     3961 cggagtcgat ccacggtcac tgtccgatgg tgagccgggc catagaggtc gaccgtgtat
     4021 tgcccggccc tggtcaggcg gctgaccaga tcgaagtcct tgtcgacgaa gaccaggtcc
     4081 cacccggcct caaaagccaa ctcgccaaga tgaccccgcc cggtactgcc ggcgccgatg
     4141 atggtgatgc gccgtggagc ggcctcagcg gttgtagtca tcttcgaccc tcacgacgtc
     4201 gtcgaggaaa ggggtctgca cctcgaacag acggaggtcc tcgagggcct cgatgcggtg
     4261 caggtcgccg ggcctgtgct ccagaatcgt acccgggccc gcgatctcag aacgcaactc
     4321 gccgtccggg c
//
LOCUS       DAOSPL010000166         4320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20415_length_4320_cov_3.236811, whole genome shotgun sequence.
ACCESSION   DAOSPL010000166 DAOSPL010000000
VERSION     DAOSPL010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20415_length_4320_cov_3.236811"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /gene="lnt"
                     /locus_tag="PLQ54_04335"
     CDS             <1..1276
                     /gene="lnt"
                     /locus_tag="PLQ54_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOS92516.1"
                     /translation="LEAAWGLILGGALYWVVGRRPSWARVVLLVPCVWVSVEWLRSLG
                     DLGIAWSDLGHSQVTAPRAVQFASVAGAYGLSYLIALTNAAIVELLTANARPVLTRVL
                     AVGPPVFLILFGQMVLALHPGDEEDAGGVQVTLVQCGEVNQPIVFGPPTPSMEQLRQF
                     IQTTQAQDIEPGGLVIWPEAAIGADLERDAVLRRAVSNLVRRKRCYLLAGCHHHTPPD
                     RSHNAAILFLPDGTIGGVYHKNRLVIMSEQTPGWLRAVAGPTLDRFGASARSLTPGDG
                     FVVFDAGDLRLAPIICFESTLTYPARRAVSRGANLIALVTNDGWFRATDGGEFHAGLS
                     IMRAVENRTPIARAACTGISMFVDAYGRISYPTPLFDMKSRAVSARIPLRPTDPPGSV
                     YTRVGEVAVWASLAAVVVSGVWGWRRRDTQAR"
     gene            1470..3299
                     /locus_tag="PLQ54_04340"
     CDS             1470..3299
                     /locus_tag="PLQ54_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015843589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOS92517.1"
                     /translation="MARCRGLPLGDVRLSGGLFAHRFALNRAYVSSLRAENLLQAFLL
                     EAGMGQWILRETRGPAPDAGDDRHWGWESPTCQVRGHFLGHWLSAAAAICVAAGDAEL
                     RARADHVVAELARCQAKNGGEWASPIPEKYMDWLAQGTPTWAPHYVHHKTLMGLHDMH
                     VLTGNAQALEVADRFADWFHRWSGQFSQKQMDDMLDVETGGMLEAWADLYGVTGSAKY
                     LDLIERYTRRRLFDPLLEGRDVLTNMHANTTIPEAHGAARCYEVTGDGRWRRAAEAYW
                     RWAVTERGTFCTGGQTSGEIWTPPMAFAARRGDKNQEHCTVYNMIRLADYLFRWTGEA
                     AYADYIERNLYNGILAQQHPGTGMVSYFLPLEAGGRKRWGHPTHDFWCCHGTLVQAHS
                     RYGRLVWFADDEGFVVSQFIPSEATAEWSGATVRLALAQDTRAGGTQALAEPRGGSTH
                     RPSAWTFRVIVTCDRPTEFGLKLRLPGWLSGPASVRVNDETPVAPPPDRGFTVLRRVW
                     SNDTVTLELPKALSVAPIPDEPGTVAFLDGPVVLAGLCDREVALECDPNDPASVLVPD
                     NEREWILWQGGYRTVKQREAIRFRPLFEVVDEPYTLYFPVRSR"
     gene            3328..4308
                     /locus_tag="PLQ54_04345"
     CDS             3328..4308
                     /locus_tag="PLQ54_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92518.1"
                     /translation="MYTSLGSAVLTSGETMDMGCVLCPDPTFAPLVPQFLAHKPESCM
                     WHIRRSVSERLDELETRFYIGRVGDEIITNIMTVESRGAGILGHVFTSVPHRRKGACK
                     LLMAEQMGDFRRRGGQCLILDTGYDTHPYYIYRGFGFESIAPEAGQMRYTVVGDDAFA
                     AEWFSAEPVSVVDLAWHHWPAISVMTSFDYGEYLRTVLPPAFGPYNFEGPFYGLKQRC
                     EERGDCQCKVLQGPNGAVLGYAALQPDVRFHEEVYVLDLMLWPGAAEHGAALVSAFDW
                     PKAKVQAYADDASEAKIRALNAAGFVDEAILRKQIHRGGKPMDVLVFARE"
BASE COUNT          651 a         1447 c         1500 g          722 t
ORIGIN      
        1 gctcgaggcc gcgtggggcc tcatcctcgg gggcgcgctg tactgggtcg tcgggcggcg
       61 cccctcgtgg gcacgcgtgg tgctcctcgt gccctgcgta tgggtgtcgg tcgagtggct
      121 ccgctcgctg ggcgacctcg gtatcgcgtg gagcgacctg gggcactccc aggtgacggc
      181 gccccgggct gttcagttcg ccagcgtggc cggtgcatac ggactctcgt acctgatcgc
      241 cctgaccaac gcggcgatcg tcgaactgct caccgccaac gcccggccgg tgctgacgcg
      301 cgtgctcgcg gtcgggccac ccgtgttcct catcctgttc ggccaaatgg tgctggcgct
      361 ccacccgggc gacgaggagg acgccggcgg cgtgcaggtg accctcgtgc agtgcggaga
      421 ggtgaaccaa cccatcgtct tcggcccacc gacgccgtcc atggagcagt tgaggcagtt
      481 catccagacc acccaagcgc aggacatcga gccggggggg ctcgtgatct ggcccgaggc
      541 cgccattggg gccgatctcg agcgcgacgc ggtcctgcgg cgagcggtga gcaacctggt
      601 ccggcggaag cggtgctatc tcctcgccgg atgccaccac cacacgccgc ccgaccgcag
      661 ccacaacgcc gccattttgt tcctgcccga cgggaccatc gggggcgtct accacaagaa
      721 ccggctcgtc atcatgagtg agcagacgcc gggctggctg cgcgccgttg ccgggccgac
      781 actggatcgt ttcggcgcat cggcccggag cctcactccg ggcgatggct tcgtcgtgtt
      841 cgatgcaggc gacctgcggt tggcgccgat catctgcttt gagtccacgc tgacctaccc
      901 ggcgcggcgg gccgtgagcc gtggggccaa cctcatcgcc ctggtgacca acgacggctg
      961 gttccgtgcc actgacggtg gcgagttcca tgccgggttg tccatcatgc gggccgtcga
     1021 gaaccgcact cccatcgccc gcgcggcgtg cacgggtatc tcgatgttcg ttgatgccta
     1081 cggccggatc tcctatccca cgccgctgtt cgacatgaag agccgggccg tcagcgcccg
     1141 catcccgctc cggcccaccg acccgcccgg gtcggtgtac acgcgcgtgg gcgaggtggc
     1201 cgtatgggcg agcctggccg ccgtggtggt gtcgggggtg tgggggtgga ggcggcgcga
     1261 tactcaggcg cgatagagcc ggatggggcc gctcatgccg cgcgtcccga ttcctcctcg
     1321 ggccctccgc ccgcccgctc gcgtgcggcc tcggtacgag gcaacaggtc aaggggagcc
     1381 ggcccgctcc gtggaacggt cagagcaaag agtgccggtg gcgcgagcga tgccgtgcgc
     1441 gaccgatccg gctttcggag gactgaggca tggcgagatg ccgtgggctg ccgctgggtg
     1501 atgtgcgtct atcgggagga ctgttcgccc atcggttcgc actgaaccgc gcctatgtat
     1561 cgagccttcg cgcggagaac ctgctccagg ccttcctcct cgaagcaggc atggggcagt
     1621 ggattctccg ggagacgcgc gggcccgcgc ccgatgccgg cgacgaccgt cactgggggt
     1681 gggagtcgcc cacctgtcag gtcaggggcc acttccttgg ccactggctc tcggccgccg
     1741 ccgcgatctg tgtcgccgca ggcgatgcgg agctgcgggc ccgcgccgac catgtggtcg
     1801 ctgagctggc ccgctgccag gccaagaacg gcggcgagtg ggccagcccc attccagaaa
     1861 agtacatgga ctggctcgct caaggcacgc ccacctgggc gccgcactac gtgcaccaca
     1921 agacgctcat gggactccac gatatgcacg tgctcaccgg gaacgctcag gcgctcgagg
     1981 tggccgaccg gttcgcggac tggttccacc ggtggtcggg ccagttctcc cagaagcaga
     2041 tggacgacat gctcgacgtc gagaccggcg ggatgctcga ggcctgggcc gacctgtatg
     2101 gtgtcaccgg ctccgccaaa tacctggacc tcatcgagcg ctacacgcgg cgccggctgt
     2161 tcgaccctct gctcgagggt cgcgacgtgc tcacgaacat gcacgcgaac accacgatcc
     2221 ctgaggccca tggcgcggcc cggtgctacg aggtgaccgg cgacggccgg tggcgccggg
     2281 ccgcggaggc ctactggcgg tgggcggtca cggagcgcgg caccttctgc accggtggac
     2341 agacctcggg cgagatctgg acccctccca tggcctttgc cgcccgccgg ggcgacaaga
     2401 accaggagca ttgcacggtc tacaacatga ttcggctggc ggactacctc ttccggtgga
     2461 ctggcgaggc ggcctatgcc gactacatcg agcgcaacct ctacaacggc atcctcgccc
     2521 agcaacaccc gggcacgggc atggtcagct acttcctgcc tctggaggcc ggtgggcgca
     2581 aacggtgggg gcaccccaca cacgacttct ggtgctgcca tgggaccctg gttcaagccc
     2641 acagccgcta cgggcggctc gtgtggttcg cggacgacga ggggttcgtc gtgtcgcagt
     2701 tcatcccgag tgaggcgacg gccgagtgga gcggcgcgac cgtgcggctg gcgctggcgc
     2761 aggacacccg cgcggggggg actcaggcgc tcgccgagcc acgtggcggc tcgacgcatc
     2821 gtccttcggc gtggacattc cgggtgatcg tcacctgcga ccgccccact gagttcgggc
     2881 tcaaactccg gctccccggc tggctgagtg ggccggcctc ggttcgcgtc aacgatgaga
     2941 cgcccgtggc ccccccaccc gatcggggct tcacagtgct ccggcgtgtc tggtcgaacg
     3001 acaccgtgac gctggagctg cccaaggcgc tgtccgtggc ccccatcccc gatgagccgg
     3061 ggaccgtggc attcctggat ggcccagtgg tgctcgccgg cttgtgcgac cgcgaggtcg
     3121 cgctggagtg cgacccgaac gatccggcca gtgtgctagt gcccgataac gagcgagagt
     3181 ggatcctgtg gcagggaggc taccgcaccg tgaagcagcg cgaggccatc cggttccgac
     3241 ctctctttga ggtggtcgat gaaccctata ctctctactt cccggtgcgc tcacgctgag
     3301 caccggtcgt gacacggagg gtgatccttg tacacgagcc tgggtagcgc tgtcctgacg
     3361 tcgggcgaga ccatggacat gggttgcgtg ctgtgtcccg atccgacgtt cgcaccgctg
     3421 gtcccgcagt tcctcgccca caagcccgag agctgcatgt ggcacatccg gcggtccgtg
     3481 agtgagcggc tggatgagct cgagacccgt ttctacatcg gccgggtggg cgatgaaatc
     3541 atcacgaaca tcatgaccgt cgagtcgcgg ggcgccggta tcctcggaca cgtcttcacc
     3601 tcggtgcccc atcgccgcaa gggcgcctgc aagttactga tggccgagca gatgggcgac
     3661 ttccgccgcc ggggtgggca gtgcctgatc ctcgacacgg gctatgacac ccacccgtac
     3721 tacatctaca gaggtttcgg cttcgagagt atcgccccag aggccggcca gatgcgctac
     3781 accgtcgtcg gcgacgatgc cttcgccgcg gagtggttct cggccgagcc ggtgagcgtg
     3841 gtggatctgg cctggcacca ctggccggct atctcggtga tgacctcatt cgactacggg
     3901 gagtatctgc gcacagtgct gccaccggcc ttcggcccgt acaacttcga ggggccgttc
     3961 tacggcctga agcagaggtg cgaggagcgc ggcgactgcc agtgcaaggt cttgcagggc
     4021 cccaacggcg ccgtgctcgg ctacgcggcc ctgcagcccg acgtgcggtt ccacgaagag
     4081 gtctacgtgc tcgacctgat gctgtggccg ggtgctgccg agcacggcgc tgccctggtg
     4141 agcgccttcg actggccgaa ggccaaggtg caagcctatg cggacgacgc gtcggaggcc
     4201 aagattcggg cgctgaacgc ggccgggttc gtcgatgagg cgattctgcg taagcagatc
     4261 caccgcggcg ggaagccgat ggacgttctc gtgttcgcgc gggagtagag acgccaaatc
//
LOCUS       DAOSPL010000167         4299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20535_length_4299_cov_2.687559, whole genome shotgun sequence.
ACCESSION   DAOSPL010000167 DAOSPL010000000
VERSION     DAOSPL010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20535_length_4299_cov_2.687559"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PLQ54_04350"
     CDS             <1..469
                     /locus_tag="PLQ54_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92519.1"
                     /translation="VRLLWGVLPVPYADVTEVWMASRRARARVYLAGPLSTLVFGSAM
                     ALAAWLIPPSPVRAIAHASATASLALAAATLCPFLGYASDGYYVLASHWGSPSLRQDA
                     LRLVARALSRRVRQKGFSPHERRLLVFAAATLGCLSAMVGCAVWGCLARVSGP"
     gene            486..1013
                     /locus_tag="PLQ54_04355"
     CDS             486..1013
                     /locus_tag="PLQ54_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOS92520.1"
                     /translation="MGSESGLGGQIAFLRTVPLLRHARLADLEALAGWASERTYGRGQ
                     IIFREGQPGRELYIVRSGEVIVSQRLRGRVENVIARFGPREFLGELGWITGSPRTATA
                     QAHCDSTLIHVPGERLFEVIETAPETACRLLLALAEEFAERISETNQRLRQAVLWGLD
                     ATGYPDDAVPPGEVT"
     gene            1014..2078
                     /locus_tag="PLQ54_04360"
     CDS             1014..2078
                     /locus_tag="PLQ54_04360"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HOS92521.1"
                     /translation="MGIRPYRSFGCRISDEFTYRGLRVCVLENELLRVSVLVDQGTDI
                     FEFQYKPVGIDLLWQSPRGVQPSAPFIASREAAHGSFSDFYEGGWQECFPNGGRSCHY
                     RGADLGLHGEVFGLPWECSVIEDSPSHVAARFCVRTPRTPFFLSKTLHLEAASPVLRI
                     EEELVNECNEAMDCMWGHHPAFGAPFLSGSCIITTGAKTAVLHDADDPQSRFAAPLSA
                     AYPRVPDRRGQPVDISKVPDRHATVSDMFYLTDLAEGWYAVTNTDLGLGFAMTFDPSV
                     FRHIWYWIACNAPSGSPFYGRAYTVALEPFTSFPAVLADAIEAGRQLTLPPYGAVRTW
                     LAAGVFEARVPPQHRTQLPC"
     gene            2268..3413
                     /locus_tag="PLQ54_04365"
     CDS             2268..3413
                     /locus_tag="PLQ54_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOS92522.1"
                     /translation="MTCREARDLVRQHLAGTLDAAWTSHLERHLATCDACETLLDELL
                     ALQPGTAGEAIPEGLHHDVLALVIDQPAGLADDCYLMAPLLSAAIDGELDHADAATLQ
                     VHLDGCVSCRGALERMRSLNASIRALPWAEPPRALDRRIHALIPMPSRSLGRRVAAWL
                     RRLPAAARPAHAALPLAAAAALALIIALPRVQQPSPTALSPTVGPASFEGSASPDVEM
                     PAVVSDTVSSPVAPQPAAPARVAAVGAPSPRPRTERAARVLLDRARSVGSRLVTVATA
                     TASAPIQARGSVETVADDVVVLKLDRHEKSPVLRYTSLARMPELYGYEPDTLRPDITG
                     LADGGAPRREPMEAVAEVVANLDHYDSFAGGPQPLPAMAMTAGLLYQ"
     gene            3533..>4299
                     /locus_tag="PLQ54_04370"
     CDS             3533..>4299
                     /locus_tag="PLQ54_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92523.1"
                     /translation="MVLRSVIHRAGSLVLVATLLLLGAQAVGALELRVDSDLVLERPR
                     TPGLNVGRASTGMANIALTDEAYRVLRSPVALSESGWMNSTTLSSSSGLSAFGPSLTQ
                     RFVASTLSDSAITEGLPKLSTYSKTAPVESMSLLDRRTGRAPEEQVYGASVTGVTLGK
                     TQVSYGLVGQAPEVLTASSLRAAERSLGGSMGPYARWLGLSFGGAESAVRGYFRSANI
                     DQGWAPSSASGDAAIVNEFRTLGFADKQDLFDGNINRT"
BASE COUNT          610 a         1417 c         1483 g          789 t
ORIGIN      
        1 tgtacgcttg ctctggggcg ttctgcccgt cccatacgcc gacgtcaccg aagtatggat
       61 ggccagccgc cgggctcgcg cgcgggtgta tttggccggg ccgctttcga ccctggtttt
      121 cggctcggcc atggcgctgg cggcctggct cattccgccg tcgcccgtcc gggccatcgc
      181 tcacgccagc gccacggcgt cgctggcact ggcggccgca acgctttgtc ccttcctggg
      241 ctacgcttcc gacgggtact acgtgctggc cagccactgg ggctccccca gcctgcgaca
      301 ggacgcgctg cggcttgtgg cgcgcgccct gtctcgtcgc gttaggcaga aggggttctc
      361 accccacgaa cgaaggctgc tggtgttcgc cgcggctacc ctgggctgcc tcagcgccat
      421 ggtgggatgt gccgtgtggg ggtgcctggc ccgcgtctca ggcccataga gcaggagaat
      481 gccgcatggg atccgagagt ggcctcggcg ggcagatcgc gttccttcgg acggttccgc
      541 tcctgcggca cgcacgcctg gccgatctcg aagcgctcgc cggctgggct tcggagcgga
      601 cctacggccg gggtcagatc attttccgcg agggccagcc gggccgggag ctctacatcg
      661 tccggtcggg agaggtgatc gtgagccagc gcctgcgtgg ccgggtcgag aacgtgatcg
      721 cccgcttcgg tccccgcgag ttcctgggcg agctgggctg gatcacgggt tctccccgca
      781 cggcgacggc acaggcccac tgtgattcca cgctcatcca cgtgcccggc gagcggctct
      841 tcgaggtgat cgagaccgct ccggagaccg cctgccggct gctcctcgct ttggcggagg
      901 agttcgccga gcgcatcagt gagaccaacc aacgcctgcg ccaggccgtt ctgtgggggc
      961 tggacgcgac cggttacccg gacgacgccg tgccgccagg agaggtgacc tgagtgggaa
     1021 tccgcccata ccgttcattt ggttgccgca tcagtgacga gttcacatac cgaggtctgc
     1081 gcgtctgcgt gctcgagaac gagctgctgc gcgtgagtgt cctggttgac caagggaccg
     1141 acatcttcga gttccagtac aaaccagtgg gtatcgacct gctgtggcag tcgccccgcg
     1201 gagtgcagcc ctccgccccg ttcattgcga gtcgggaggc ggctcacggc agcttctccg
     1261 acttttacga gggtggctgg caggagtgct tccccaacgg cggccggagc tgccattacc
     1321 gcggagcgga cctggggctg cacggcgagg ttttcggtct cccgtgggag tgctcggtca
     1381 tcgaggattc gccgtcgcac gttgccgcgc ggttctgtgt ccgaacaccg cgcactccct
     1441 ttttcctcag caagacgctg catcttgagg cggcgagccc cgttttgcgg attgaagagg
     1501 aactggtcaa cgagtgcaat gaagcgatgg attgcatgtg gggtcatcat cctgcattcg
     1561 gcgcaccttt cctatcgggc tcctgcatta tcacgaccgg tgcgaagact gcggtgctgc
     1621 acgatgccga tgacccccag agccggtttg cggcccccct cagcgccgcc tacccacgcg
     1681 tacccgaccg tcgcggtcag ccggtggaca tcagcaaggt ccccgatcgc catgcgacgg
     1741 tgtcggatat gttctacctg acggacctag cagagggatg gtatgcagtt accaatacag
     1801 atctgggtct ggggttcgcc atgacctttg atcccagcgt gttccgccac atctggtact
     1861 ggatcgcctg caacgcgccg tcgggttctc cgttctacgg ccgcgcttac acggtggctc
     1921 tcgagccctt cacgtcattt ccggccgtcc ttgcagacgc catcgaagct ggccgccagc
     1981 tcactctgcc gccctatggg gccgtgcgga catggctggc cgccggagtg ttcgaagccc
     2041 gggtcccgcc gcaacatcgg acacaactcc catgctaggc aggtgccgtg cggtgacaaa
     2101 agaatccgcg gattgggtgc aacatgcgct gccctgacgg gtccaagtcc atgcgggcac
     2161 ccgggtaggg cgggccacgg aaccggtgcc ggagacgggc tctgccccgt gagggcgcgt
     2221 gagccggtga gggtgcagca gagacgtggg agggaggcga ggggccgatg acgtgtcgtg
     2281 aggcgcgaga cctcgttcgg cagcatctgg cgggcacgct cgatgccgcg tggacgtcgc
     2341 atctcgagcg gcacctcgcc acctgcgacg cctgtgagac cctcctggat gagctgctgg
     2401 cgctgcagcc ggggactgcg ggcgaggcga ttcccgaggg acttcaccat gacgtgttgg
     2461 ccttggtgat cgaccagccg gcgggcctgg ccgacgactg ctacctcatg gctccgttgc
     2521 tgtcggctgc cattgatggc gagctcgacc acgcggatgc cgcgacgctc caggtgcacc
     2581 tggatggttg tgtgagttgc cggggtgcgt tggagcgcat gaggtccctg aacgccagca
     2641 tccgcgccct gccgtgggcc gagccgcctc gtgctttgga tcgccggatc cacgccctga
     2701 tcccgatgcc ctctcgctcg ctgggccgcc gcgtggcggc gtggctgcgt cggctgcccg
     2761 cggccgcgcg gcccgcccat gcggcactgc cgctcgccgc cgcggcggcc ctggctctca
     2821 tcatcgcact cccccgggtg cagcagccgt cgccgacggc gctgagcccg acggttggcc
     2881 ctgcgtcatt cgaggggagc gcctcgcctg acgtggagat gccggctgtg gtgagcgaca
     2941 ccgtgtcgag tccggtggcc ccacagccgg cggcgccagc ccgtgtggcg gcggtcggcg
     3001 ccccgagtcc gaggccccgc accgagcgcg ctgctcgtgt ccttctggac cgggcccgct
     3061 cggtggggtc acgcctggtg actgtggcta cggctaccgc ctcggcaccg atccaggctc
     3121 ggggctcggt cgagactgtc gctgatgatg tggtggtact gaagctcgat cggcacgaga
     3181 agagcccggt cctgcgttat acttctttgg cgcggatgcc tgagctttat gggtatgagc
     3241 cggacacgct gcggcccgac attaccgggc tggcagacgg cggcgcccca cggcgcgaac
     3301 cgatggaggc tgtggccgag gtggtggcca acctcgacca ctacgacagt ttcgcgggcg
     3361 ggccccaacc gctccccgcg atggccatga cggcggggct gctctaccaa tgaggagccc
     3421 actgccctgg agctgccaac ggaatcccgg gtggatggac tggcgtctgg gccggtctcg
     3481 aggtcccgca cttgaaccgg aacgcctggc tcgccgttgg gatgggttag atatggttct
     3541 acgaagtgtg atacatcgag ccgggtcgct tgtgctggtg gcgacgcttc tgctgctggg
     3601 tgcccaggcg gtgggcgctc tggagttgcg cgtcgacagt gaccttgtcc tcgaacggcc
     3661 ccgtactcct ggcctgaacg tgggcagagc ttccacgggc atggcgaaca ttgccctgac
     3721 cgacgaggcc tatcgtgtgc tgcgcagccc cgttgccttg agtgagagcg ggtggatgaa
     3781 ctcgaccaca ctctccagca gctcgggcct gtcggcgttc ggcccgtcgc tgactcagcg
     3841 cttcgtcgca agcacgctct cggactccgc catcacggaa gggctcccga agctatcgac
     3901 ctactcgaag acggctccgg tcgagtcgat gagcctgttg gaccgccgga ccggccgcgc
     3961 gcctgaggag caggtctatg gtgcgtcggt gaccggcgtc accctgggca agacccaggt
     4021 ctcctatggc ctcgtgggcc aggctcccga ggtgctgacc gcatcgtcgc tcagggctgc
     4081 ggagcgctcg cttggcgggt ccatggggcc atacgctcgc tggttgggct tgagtttcgg
     4141 cggggccgag agcgccgtgc gtggctactt ccgctcggcg aacatcgacc agggctgggc
     4201 gccgtcgagc gccagcggcg atgccgccat cgtcaacgag ttccgcacac tgggcttcgc
     4261 ggacaagcag gacctctttg atggcaacat taaccgcac
//
LOCUS       DAOSPL010000168         4284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20623_length_4284_cov_3.071412, whole genome shotgun sequence.
ACCESSION   DAOSPL010000168 DAOSPL010000000
VERSION     DAOSPL010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20623_length_4284_cov_3.071412"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PLQ54_04375"
     CDS             complement(<1..727)
                     /locus_tag="PLQ54_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOS92524.1"
                     /translation="MAQVTPTDGRPATSKEVGRRARVAPRVASLPPSGIREFFDLVQT
                     MDDVISLGVGEPDFRTPWHVCDEIIDSLERGRTAYTSNQGIIELRTAISGYLQRLYGV
                     EYDPNSEVLVTNGVSEGIDIAMRATLCPGDSVLVPEPCFVSYSACAWLAGGEAIPVPT
                     RIENGFRLRREDLEAAVRPNTRSLMLGYPSNPTGAVMTRAEMGEVAQFVLDHDLLVYS
                     DEIYDRLIYDGHQHVCFATLPGMR"
     gene            complement(807..1310)
                     /locus_tag="PLQ54_04380"
     CDS             complement(807..1310)
                     /locus_tag="PLQ54_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOS92525.1"
                     /translation="MADRANLKVDIARILEADCRLSPDEIGRMLAVPADVVAREIAEM
                     EASGAIVRYSARINWDKLDGPERVFAFIGVQATPEREVGFDGIARRLVHFPEVHSLYL
                     ISGAQDFDVVLQGSSMRDIAHFVARRLAVIPGVRSTATQFVLQTYKRDGDVLMDAEKP
                     DRLSVTP"
     gene            complement(1320..2900)
                     /locus_tag="PLQ54_04385"
     CDS             complement(1320..2900)
                     /locus_tag="PLQ54_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOS92526.1"
                     /translation="MPFPPTRRLRWLVQVLFLIGFVVLLDITVVPLPEGLPTDLYLRL
                     SPLNGLVTMLARREILYTTLLPGLATLLITALFGRVFCGWFCPMGTTIDVFERLALRR
                     RRYPLPDPKRLDRTRPIKYVLLTGMLAAALLSFQPAAFLDPISMVHRSYIAVVHGPIH
                     NLAQDARDVAYRPLRARGRRIQPGAAPRVYHLNAVILAMWGTVLGLSAVQKRFWCRHL
                     CPLGALLALVAHLPLLRRRVADGCTHCMRCERDCKMGCIQEKGRSYRSRECILCYNCE
                     SICPVDAVSFPLGAGPEPGRDSSHQLGRRRLLGGAAAGVLWMVGVRTSVGHARTAGRS
                     DLPNQLEENTKSPRRIRPPGALPERQFLQTCIRCGECMKVCPTNALQPALGEGGLEGL
                     FTPILVPRIGHCEERCNACSRVCPTGAIQNFRIEEKRDKFILGKASINRSTCRPWYGG
                     KKCSICDEHCSYDAIDTRMIDGAERPVVLEDKCTGCGICERMCPVTPVAAIEVSARDE
                     RRPLVDEGLSPLSGFTSQ"
     gene            complement(2906..3880)
                     /locus_tag="PLQ54_04390"
     CDS             complement(2906..3880)
                     /locus_tag="PLQ54_04390"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOS92527.1"
                     /translation="MAAATAGSLAYDLVIARKAPAAELVRLAFDQLGGMDKLVRPGQQ
                     VCLKPNMAWAREKGSGACTNPDVLGAVIDLILQAGVAAKDIVVFEHTRDAFTTAASFS
                     EIQAVCDARRIALVNGDSQQVYDAQEAPEGTKFLKPFGENEEVASDLMEADVVFNMPV
                     LKQHSATGLSMGMKNLMGAIYNPRRYHGGKDADPDSGSQELDERIVDLTVLLKPKLTL
                     TVLDATFVLREAGPKGDIGTPGDELQTLIVGTDPVAVDSAGAQIFGMAPDKVGHVKAA
                     ADRKVGEMDVDKLRASDRLAEIDGPAGPAKGQADADGAAPTDSSDKAG"
     gene            complement(4098..>4284)
                     /locus_tag="PLQ54_04395"
     CDS             complement(4098..>4284)
                     /locus_tag="PLQ54_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92528.1"
                     /translation="KCGDTDAAMETLRRAVERVAAPSVRARCHYQLSRILEAQGRAAE
                     AKEHLALARDLGYPLAR"
BASE COUNT          729 a         1482 c         1373 g          700 t
ORIGIN      
        1 cgcgcatgcc cggcaaagtg gcgaagcaca catgctgatg cccgtcatag atcaggcgat
       61 catagatctc gtccgagtag accagcaggt catggtcgag cacgaactgg gccacctcac
      121 ccatctcggc gcgggtcatg acggccccgg tcgggttgct ggggtagccg agcatgagcg
      181 agcgcgtgtt cggacgaacg gcggcctcca ggtcttcacg gcgcagccgg aatccgttct
      241 cgatccgcgt ggggaccgga attgcctcgc cgcccgccag ccaggcgcag gcgctgtagg
      301 agacgaagca gggctccggc acgagcacgc tgtcgcccgg acacagcgtg gcccgcatgg
      361 cgatgtcgat cccctcgctc accccgttcg tgacgaggac ctcggagttg ggatcatact
      421 ccacgccata caggcgctgc aggtagccgg agatggcggt acgcagttcg atgatgccct
      481 gattgctcgt gtaggccgtg cgcccgcgct ccaacgagtc gatgatctca tcgcagacgt
      541 gccacggcgt gcggaagtcg ggttcaccca cccccagcga gataacgtca tccatggtct
      601 ggacgaggtc gaagaactcg cggatccccg aaggaggcag cgaggcgacc cgtggcgcca
      661 cacgggcccg gcgccccacc tccttgctgg ttgccggccg gccgtctgtg ggtgtgactt
      721 gtgccatcct cgcattccct cctttggtgg gtgattactc tgccggcaga cgccagcagt
      781 ccgcttcgtc acgccagatg cgctggtcag ggtgtgacgg acaaccggtc gggcttctcc
      841 gcgtccatca gcacatcgcc gtcacgcttg taggtctgca gcacaaactg tgtggccgtg
      901 ctgcgaactc cggggatgac ggccagccga cgagcgacaa agtgggcgat gtcgcgcatg
      961 ctactgccct ggagcacgac gtcgaagtcc tgggcccccg agatgagata gagcgaatgg
     1021 acctcgggaa agtgaacgag ccgccgggcg ataccgtcga agcccacttc gcgctccggg
     1081 gtcgcctgca cgccgatgaa ggcgaacacg cgctcggggc cgtccagctt gtcccagttg
     1141 atgcgggccg agtaccgaac gatggcgccg gacgcctcca tctcggcaat ctcacgggcc
     1201 acaacgtccg cgggcaccgc cagcattcgg ccgatctcgt cgggcgacaa gcggcagtcg
     1261 gcctccagaa tgcgggcgat gtctaccttc aggttggcgc ggtcagccat gatgactcct
     1321 cactgactcg tgaaccccga cagcggcgac aggccctcgt ccaccagagg acgccgttcg
     1381 tctcgggcgc tcacctcaat cgccgccaca ggggtgaccg ggcacatccg ctcgcagatg
     1441 ccgcagccgg tgcacttgtc ctcgagaacc acggggcgct cggcgccgtc gatcatccgg
     1501 gtatcgatgg catcgtacga gcagtgctcg tcacagatcg agcacttctt gcccccatac
     1561 cagggccggc aggtcgaacg gttgatcgat gccttgccca ggatgaactt gtcgcgtttc
     1621 tcctcgatgc ggaagttctg gatcgcgccg gtggggcaga cccgagagca ggcgttgcac
     1681 cgctcctcac aatgcccgat gcggggaacc aggatgggcg tgaacagccc ttccagcccg
     1741 ccctcgccca aagcgggttg cagggcgttg gtcgggcaca ccttcataca ttcgccacag
     1801 cggatgcacg tctgcaggaa ctggcgctcg ggcagggccc ccggtggccg gatgcgtcgg
     1861 gggctcttcg tgttctcctc gagctggttt gggagatccg agcgtccggc ggtgcgcgcg
     1921 tgacccaccg atgtccgtac gcccaccatc cagagcaccc cggccgccgc tccgcccagc
     1981 aggcgccgtc ggcccagttg gtgggaggaa tcccgaccgg gctcgggccc ggctcccaga
     2041 gggaacgaga cggcgtccac gggacatatg gactcgcagt tgtagcagag aatgcactcc
     2101 cttgagcggt acgatcggcc cttctcctgg atgcagccca tcttgcagtc gcgctcgcac
     2161 cgcatgcagt gagtgcaccc atcggccacg cgccggcgca acaacggcag gtgcgccacc
     2221 agagccaaca gcgcccccag ggggcacaga tgacggcacc agaagcgctt ctggacggca
     2281 ctcagaccga gcactgtgcc ccacatcgcc aggatcacag cgttcagatg gtacacgcgc
     2341 ggagcggcac ccggctggat ccggcgccct cgggctctca gcggcctgta ggctacgtcc
     2401 cgggcgtcct gcgccaggtt gtggattggg ccgtgcacca ccgcgatgta gctccggtgc
     2461 accatactga tgggatccag gaacgcggcc ggctggaagg acaggagggc cgccgccagc
     2521 atcccggtca gtaggacata cttgatgggc cgcgtgcggt ccagtcgctt cgggtccggg
     2581 aggggatatc tgcgtcgccg cagggccagc cgctcgaaca cgtcaatggt cgtgcccatg
     2641 gggcagaacc atccacagaa cacccgccca aacagcgcag tgatcagcag cgtcgccagc
     2701 ccaggtagca gcgtggtgta caggatctcc cgccgggcca gcatcgtgac gaggccgttg
     2761 agtgggctca gccgcaggta cagatcggtc ggcaggccct ccggtagcgg gaccacggtg
     2821 atgtccagca ggaccacgaa accgatcaga aagaggacct gcaccagcca gcgcaggcga
     2881 cgagttggtg ggaatggcac gatggctagc ccgccttgtc cgatgagtcg gtgggggccg
     2941 ccccatccgc atcggcttgc cccttggcgg ggccggccgg cccgtcgatc tcggccagac
     3001 gatccgatgc gcgaagcttg tcgacgtcca tctccccaac cttgcggtcg gcggccgcct
     3061 tgacgtgccc caccttgtcg ggcgccatgc cgaagatctg agccccggcc gagtccaccg
     3121 ccactggatc tgtccccacg atgagcgtct gcagctcatc gcccggtgta ccgatgtccc
     3181 ccttgggccc ggcttctcga agcacgaaag tggcgtccag caccgtcaga gtgagcttcg
     3241 gcttcagcag caccgtcaga tcgacgatgc gctcgtccaa ctcctggctg ccggagtcgg
     3301 gatccgcgtc tttcccgccg tggtaccggc gtgggttgta gatggctccc atcaggttct
     3361 tcatgcccat cgacagcccg gtagccgagt gctgcttgag caccggcatg ttgaacacca
     3421 cgtcggcctc cattaggtct gaggcgactt cttcgttctc accgaatggc ttgaggaact
     3481 tggttccctc cggggcctcc tgcgcgtcgt agacctgctg gctgtctccg ttgaccagag
     3541 cgatccggcg ggcgtcacag acggcctgga tctcgctgaa cgaggccgcc gtggtaaagg
     3601 cgtcccgagt gtgctcgaac accacgatgt ccttagccgc cacaccggcc tggaggatca
     3661 ggtcgatcac ggcacccagc acgtccgggt tcgtgcaggc acccgagccc ttctcgcgtg
     3721 cccacgccat gttcggtttg aggcagacct gctgacccgg ccggaccagc ttgtccattc
     3781 ccccgagctg atcgaacgcc agacgaacca gctcagccgc cggagccttc cgcgcaatca
     3841 cgaggtcgta ggccaaggaa ccggcggtcg ccgcggccac cctcggccgg gtcccactcc
     3901 ccccggcacc ttcctgcggc gccccattgc cgcttcgctt tccacagccg agggagccaa
     3961 ccccaaggag cgcgcctcct cccacgagtg cggtggcacg cttcaaaaag tctctgcggc
     4021 agatggtgag tggaactctc atacggcgtg acatcccctc ccgcagcggc cgcccccgcg
     4081 cccgcgccgt accgtgacta ccgcgctaga gggtacccga ggtcccgagc cagggcaagg
     4141 tgctctttcg cctccgcggc gcggccctgc gcctccagga tgcggctcag ctggtaatgg
     4201 caccgcgccc tgaccgaggg cgcggccaca cgctccacgg cccgccgcag cgtctccatg
     4261 gccgcgtcgg tgtcgccaca cttc
//
LOCUS       DAOSPL010000169         4281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20645_length_4281_cov_3.001420, whole genome shotgun sequence.
ACCESSION   DAOSPL010000169 DAOSPL010000000
VERSION     DAOSPL010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20645_length_4281_cov_3.001420"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1478
                     /locus_tag="PLQ54_04400"
     CDS             <1..1478
                     /locus_tag="PLQ54_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92529.1"
                     /translation="LVSDGTVWFDDVCLSPRESVAEVTLDIERPNPRGLVRQGVDDAV
                     VRAVCLAKTPIEGASASVTLSDRAGTVLERRGLAVQDEPSEVTFDMNQAAIGGYTVHA
                     EITSADGQVLAAEAAHVDIVPADAPGVFLGAHGVAAANGTPWFPIGLTSISPLDDEAA
                     GLAAAGFNLLVPGTFSQGTPEDAQRLLDRAEELGLYVMEWNNGWVYPTGQVTAEAREQ
                     ALGKLAENVGGHPAFLGLMCDEAIWNGVPLADVAHAYHTFRRLMPTKLFWQNQAPRNT
                     IEDLARYCRFADVSGMDIYPVEGSSHSDLPNQTLSVVGDEVDKNRETVAGRKPIWAIL
                     QGFGWSVWEADPATHKRTPTWEETRFMAYDSVVHGAVGIIYWGASYEKRDSDIWTSLR
                     RIAGELRDLAPALTATEWVDIAAASEDNAVRATGRRVGDRLFVIAVNESDTAVEARLS
                     LPGGVTRLARVAEEGPALAARDATVTDAFEPWGVHVYSSVE"
     gene            complement(1768..4146)
                     /locus_tag="PLQ54_04405"
     CDS             complement(1768..4146)
                     /locus_tag="PLQ54_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92530.1"
                     /translation="MSLEAAPKLRRVQSLDGDWKLWNTDPTPAGQEAAACGQAALDAI
                     PATVPGDIHLDLMAAGRVADLFVGDNYKQALFAEEQDWWYERTFVPDTQLAGADRVEL
                     VFDGLDTFATVFLNGMAIGQSRNMWVPLRLDVTRLLRHGESNTLTVVLRSPRKSVEAD
                     MAAEHPEPINAFFSPPERVWCRKAQMSFGWDIAPRIVTCGIWRPVRLEGHFAGAIRDV
                     WARTRLVEGGQAELALSVEADALAAGGEWSARFRAACGESVVEGQLPLLATCGRLAGL
                     ATVAVPSPRLWRTWDIGTPNLYDLTVELRADGNAVDEWQGRFGIKDIRLVLTEPDDGR
                     NCFRFELNGRPTFMRGSNWIPVDALFARVTPERLEAVIDLAIDTGCNMLRVWGGGIYE
                     HPAFYRLCDERGILVWHDFMYACGLYPQSEAFLSAARREAEWVVRSLRNHPCIALWAG
                     DNECDCGYGWDGDVGRYVDNRLTRRVLSSVVAELDPDRPYLPSSPCNPSDKGDPNTAD
                     EGDVHIWSHSEHPRAPMYSQERSLFISEMGRICAANVESTKRFLPPESQWPHANPVWD
                     QHLGTIPTSDFRRRDRMDEGIGAVFGFVPDTLEEYVEASQLMQAFCLSEWIQRARRRK
                     WECGGILWWNLFDNWPQHADAIVDYYFEKKIAYAAVRDSSRLVMPTVALNEDGDGYEV
                     WLLNDTDGAVEGVVALALGVDGSSRTLLQTPGRAEANASTLAVTVPVEVLGRVDANHL
                     YLVATLDTGQGGVIATPHILNGCASREVLRAVFGAQSAGRTPDPARAYAPGT"
BASE COUNT          710 a         1528 c         1358 g          685 t
ORIGIN      
        1 agctggtctc cgatggcacg gtctggttcg acgatgtctg cctatctccc agggaatcgg
       61 tggccgaggt gacgctcgac attgagcgcc ccaacccgcg tggccttgtg cgccagggcg
      121 ttgatgacgc cgtagtccgc gctgtgtgcc tggcgaagac cccgatcgag ggtgcctcgg
      181 cctcagtgac cctctccgac cgcgccggga ccgtcctcga gcgccgcggg ttggcggtgc
      241 aggatgagcc ctccgaggtc acattcgata tgaaccaagc ggctattggc ggctacaccg
      301 tccacgcgga gatcaccagt gcggacgggc aggtactggc cgccgaggcg gcgcacgtcg
      361 atatcgtgcc ggccgacgca ccgggagtat tcctcggtgc gcacggcgtc gccgcggcaa
      421 acggcacgcc ctggttcccc atcggcctga ccagcatctc gcccctggat gacgaggcgg
      481 cgggcctcgc cgcggcgggg ttcaacctgc tggttcccgg cacgttctcc caagggactc
      541 cagaggacgc ccagaggctg ctcgaccgcg ccgaggaact cggcctctac gtcatggagt
      601 ggaacaatgg ctgggtctac ccaacgggtc aggtgacggc cgaagctcgc gagcaggcgc
      661 tcggcaagct ggccgagaac gtcggcggcc acccggcctt cctggggctc atgtgcgatg
      721 aggctatctg gaacggcgtc ccgctggccg atgtcgccca tgcctaccac accttccggc
      781 ggctgatgcc gacgaagctc ttctggcaga accaggcccc ccggaacacc atcgaggacc
      841 tggcccgcta ctgccggttc gcagacgtct cagggatgga catctacccg gtcgagggtt
      901 cctcacactc cgatctaccg aaccagacgc tcagcgtcgt cggcgacgag gttgacaaga
      961 accgtgaaac cgtcgccggt cgcaagccca tctgggccat ccttcaggga ttcggctgga
     1021 gtgtctggga ggcggatccc gccacacaca agcgtacacc cacatgggag gagactcgct
     1081 tcatggctta cgattccgtc gtccacgggg cggtcggcat catctactgg ggcgccagct
     1141 acgaaaagcg ggactccgac atctggacca gccttcgccg catcgccggc gagttgcgcg
     1201 acctggcgcc ggcgctcacg gccaccgagt gggtcgacat cgccgccgca tccgaggaca
     1261 acgccgtcag ggccaccggc cgcagggtcg gtgaccggct cttcgtcatc gccgtgaacg
     1321 agagcgacac cgctgttgag gctcgactga gcctacccgg cggtgtcacg agactggccc
     1381 gcgtcgccga agagggccca gccctggcgg cccgcgatgc gactgtcacc gacgccttcg
     1441 agccctgggg cgtgcacgtc tacagcagcg tcgagtaggg gccaccaacc ggtcgccggc
     1501 cgttcggttt ggcagccggc ggcctttgca tgtccgggca gtccggctgc ataccggatc
     1561 tacctcatgg gcgtctcggg atgagaagga agggcagagc gcggcgctct gccctcatcc
     1621 aagagacacc gaccggcgct cgtgtcgcac ccctgcagcg ccctgtcctc cggccgaata
     1681 acagggagcg tgttcctccc cgcggaagag accactgcct tgtggaggcg ccagagtgtt
     1741 acccatgtcc ccggtgcgtt ctgtcaccta tgtgcccggt gcgtacgccc tcgcagggtc
     1801 gggcgtccgc ccggcggact gcgccccaaa caccgcccgc agcacctccc ggctggcgca
     1861 tccgttcagg atgtgtggcg tcgcgatgac cccgccctgg ccggtgtcga gcgtggcgac
     1921 gaggtagaga tggttggcgt cgactcggcc gagcacttca accgggaccg tgaccgccag
     1981 ggtcgaggcg ttggcctctg cgcgccccgg tgtctgcagc agtgtgcgac tcgacccatc
     2041 cacgccgagt gcgagtgcga ccaccccctc gacggcccca tcggtgtcgt tcagcagcca
     2101 cacctcataa ccgtcgccat cctcgttcag cgcgacggta ggcatgacca ggcggctcga
     2161 atctctcacc gcggcgtacg cgatcttctt ctcgaagtag tagtccacaa tggcgtcggc
     2221 gtgttgcggc cagttgtcaa acaggttcca ccacaggatg ccgccgcact cccacttgcg
     2281 ccgccgcgct cgctggatcc actcagagag gcagaacgcc tgcatgagct gtgatgcctc
     2341 gacgtactcc tcgagtgtat ccgggacgaa cccgaacacg gccccgatgc cctcgtccat
     2401 ccggtcgcgc cggcggaagt cactggttgg gatcgtgccc aagtgttggt cccacacggg
     2461 attggcatgg ggccattggc tctcaggagg gaggaagcgc tttgtcgact ccacgttagc
     2521 ggcgcagatg cgtcccatct cgctgatgaa caggctgcgc tcctgcgagt acatcggcgc
     2581 tcgcgggtgc tcactatggc tccagatgtg cacatcgccc tcgtcggccg tattggggtc
     2641 gcccttgtcc gaagggttgc acgggctcga gggaagatag ggccggtctg gatccaactc
     2701 ggccaccaca ctcgacagca cccggcgcgt caggcggttg tccacatagc ggcccacatc
     2761 gccgtcccag ccgtagccgc aatcgcactc gttgtcgccc gcccagaggg cgatgcacgg
     2821 gtggttccgc aacgagcgga ccacccattc ggcctcgcgc cgtgccgcgg agaggaacgc
     2881 ctcgctctgc gggtagagcc cgcacgcgta catgaagtca tgccaaacga ggatcccccg
     2941 ctcatcgcac aaccggtaga acgccggatg ctcgtagatc cccccgcccc aaacgcggag
     3001 catgttgcag cccgtgtcga tcgccaggtc gatgaccgcc tccagccgct ctggcgtgac
     3061 ccgcgcgaac agcgcatcca cggggatcca gttcgacccg cgcatgaacg tcggccgccc
     3121 gttgagctca aaccggaagc agttccggcc atcgtcgggt tccgtgagca ccagccggat
     3181 gtccttgatg ccgaaacgcc cttgccattc atccacggcg ttgccatcgg cccggagctc
     3241 gaccgtcagg tcgtataggt tcggcgtgcc gatgtcccac gtccgccaca acctgggcga
     3301 cgggaccgcg acggtcgcga gccccgcgag ccggccgcac gtggccaaca gcgggagctg
     3361 gccctcgacc acgctctccc cacacgccgc gcggaaccgc gccgaccact cgccgcccgc
     3421 ggccaacgcg tccgcctcga ccgagagcgc aagctccgcc tgtccgccct cgaccagtcg
     3481 cgtgcgcgcc cacacgtcgc ggatggcgcc tgcgaagtgc ccctcgagcc gcaccggtcg
     3541 ccagatgccg cacgtcacga tccgtggcgc gatgtcccac ccgaagctca tctgcgcttt
     3601 gcggcaccac acgcgctccg gcgggctgaa gaacgcattg atcggctccg ggtgctccgc
     3661 ggccatgtcc gcctctaccg acttccgagg cgaccgcagc acgacggtga gcgtgttgga
     3721 ttcgccatgc cggagcagac gggtgacatc cagccgaagc ggaacccaca tgttccggct
     3781 ctggccgatg gccatcccat tcaggaacac cgtcgcgaac gtgtcgagtc cgtcgaacac
     3841 gagttcgacc cggtccgcgc cggccagctg ggtgtccggc acgaaggtcc gctcatacca
     3901 ccagtcctgc tcctcggcga acagcgcctg cttgtagtta tcgcccacga acaggtcggc
     3961 aacgcgcccc gccgccatga ggtcaagatg gatgtcgccc ggcacggtgg ccgggatcgc
     4021 atcgagcgcc gcctggccgc aagctgctgc ctcctgccca gccggagtgg gatcggtgtt
     4081 ccagagcttc cagtccccgt ccaaggactg cacacggcgg agcttcgggg ctgcctcgag
     4141 cgacatggcg accatcctct catcgacccg cccgattccg gcgcaccccg ctccgctcct
     4201 gccactcgga gaccggcgac tggctagaat ggcacgcact tagggtcggc acccaggcac
     4261 acccaaccgt aggcgaggct t
//
LOCUS       DAOSPL010000170         4278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20661_length_4278_cov_3.546768, whole genome shotgun sequence.
ACCESSION   DAOSPL010000170 DAOSPL010000000
VERSION     DAOSPL010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20661_length_4278_cov_3.546768"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PLQ54_04410"
     CDS             <1..216
                     /locus_tag="PLQ54_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007132362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOS92531.1"
                     /translation="EDIRQGERVREYVVEGLVGTEWRELCRGSAIGHKKIDRFPPVEV
                     AGIRLRCLESAGDPIIRKLAAYRVGGG"
     gene            317..2485
                     /locus_tag="PLQ54_04415"
     CDS             317..2485
                     /locus_tag="PLQ54_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOS92532.1"
                     /translation="MTERVSALRDRALRSRRGEDRTTPSLWQESYRRTEGLPEVTRWA
                     MATAHGYRTQPVYVQELELLVGTPPGMVYDEDGPVVPQIFGRQPFVCPWPVSDEIQRL
                     FGAGLFSPAGNHTTIDYDAIMVGGFRGLISRVDARLALPQVRADDEQTAFLGALRTIA
                     EGYIDYCGRHADEALAQAAACADPQRAQELRTIAGNCLQVVADPPRTFWQACQSAWFS
                     FVFLPDAPGRVDQYLYPFYRADMDSGRLDDDRARELLSCLWLRYLEFAGAAAAVSAFH
                     HLTLGGMRPDGSDASNQLTWLCLDVTEELRIQRPQVGLRCHADTPPALLERAVRVLRA
                     RAGNPDFCNDEQIVPALVRTGISLEDARDFSLSGCHEVIVTGRAQMGSVEGFINLPKV
                     LRAVLGLEPGLLGGATPPFPESWEQLWEQLTSAMRWVAWRAHEAALARDEEAARGAGG
                     HLQASLVVGDCVERGKGYTQGGARYNHCNWNVIGLANLTDALSAIRWLVFDQRQLTLA
                     ELAGVVSANWVGHEPLRQRVLGESPNFGNDDDRADAIATDLLRMIAAAFEGLTPFRGG
                     KYILGTLAGGENMHIEFGRMTGATPDGRRDGEPLADSAGPAQGRDRRGVTAMMNSVAK
                     LPHHLLPTATTLNVKLDPRLLETDTGLAKVAGLIRAHFAAGGQQCQFNIVDREMLLEA
                     RRDPEQYRDLMVRVAGYSAPFTSLWEDLQDEIIARTEHAL"
     gene            2621..4132
                     /locus_tag="PLQ54_04420"
     CDS             2621..4132
                     /locus_tag="PLQ54_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92533.1"
                     /translation="MFALAWALCATGASGADCFAIRVVDEATGRGVPLVALRTVDGTV
                     RYTDSAGFVAVRDPELMGQRVFFHISSHGYEYPADGFGNRGIALDVTPGGRAEVRVHR
                     VNIAERLYRLTGAGIYRDSVILGEPVPVRQPLLNARVTGQDSALAIPWRGRIYWFFGD
                     TNRIEYPLGNFHTSGATSALPGDGGLDPSIGVDIEYLVDDDGFSRAMCPMAGEGPVWL
                     DGLAVARDRDGADRLVAHYSRVRGLETLLEHGLAVFDDERQVFTKHVEFALDDTQRCP
                     RGHPVAIREDGRDWLLFPTPFASLRCEATIEALSDPGRYESFTCLAPGARFAAEHTAV
                     ERGPDGTLVWGWKPNTDAITQAQEAELAVAGLLRPDECRYQVRDGTGQAIRLHNGSLA
                     WNAYRQRWIMIVAQAGGSSSVLGEIWFAEADSPTGPWTSCTKIVTHDHYGLYNPVHHP
                     FLDQDGGRVIYFQGTYSQTFEGPVAPTPLYDYNQIMYRLDLSDARLGPPPSRR"
BASE COUNT          687 a         1405 c         1487 g          699 t
ORIGIN      
        1 gaggacatcc gccaaggcga gcgggtccgc gagtacgtgg tcgagggcct tgtgggcacc
       61 gagtggcgcg agctatgccg cggctcggcc atcgggcaca agaagatcga ccgtttcccg
      121 ccggtcgagg tcgccggcat ccggctgcga tgcctcgagt cggccggcga tccgatcata
      181 cgaaagctgg cggcgtaccg cgtcggtggg ggctagtcat ctccgaccca agagcacgac
      241 aatgagcccc aggccgtgca gtcgaaggca cgagtgctgt ttgcccggac cgacgtgtgc
      301 cgtgtggagg ccgtccatga ccgagcgcgt cagcgccctg cgggaccgtg cattgcgcag
      361 ccgtcgaggc gaggaccgga cgacgccgag cctgtggcag gagtcctatc gtcggaccga
      421 gggccttccc gaggtgacgc gctgggccat ggcgacggct cacggctacc gcacgcagcc
      481 tgtgtacgtg caggagttgg agctcctcgt cgggacgccc ccgggcatgg tgtacgacga
      541 ggacgggccg gtagtcccgc agatcttcgg tcgccagccc ttcgtgtgcc cgtggccagt
      601 ctcggacgag atccagcgcc tgtttggagc agggctgttc tcgccggcgg gaaaccatac
      661 gaccatcgac tacgatgcca tcatggtggg cgggtttcgc gggctgatca gccgtgtcga
      721 tgcgaggctc gcgttgccgc aggttcgggc ggacgacgag cagaccgctt ttctgggggc
      781 gctgcggacc atcgcggaag gctacatcga ctactgcggc cgccatgccg acgaagcgct
      841 ggcacaagcg gccgcctgcg cggaccccca gcgcgctcag gagctccgca cgatcgcggg
      901 caactgcctg caggtggttg ccgatcctcc ccggactttc tggcaggcat gtcagtcggc
      961 atggttctcg ttcgtcttcc tgcctgacgc gccggggcgt gtggatcagt acctctatcc
     1021 cttctaccgc gcggacatgg actcggggcg gctcgacgat gaccgcgcgc gcgagctact
     1081 gagctgtctg tggctgcgat atctggagtt cgcgggcgct gcggcggcgg tcagcgcgtt
     1141 ccaccacctc actctgggcg gcatgcgccc cgacggttcc gacgcgtcca accagctcac
     1201 gtggctttgc cttgacgtca cggaggaact caggatccag cgcccccagg tcgggctgcg
     1261 atgtcatgca gacaccccgc cggcattgct cgagcgggca gtgagggtgc tgcgggctcg
     1321 agcggggaac ccggacttct gcaacgacga gcagatcgtg ccggcgctgg tgcgcactgg
     1381 catttccctc gaggacgcgc gggacttctc gctctccggc tgccatgagg tcatcgtgac
     1441 gggccgtgcc caaatgggat ccgtcgaggg cttcatcaac ctgccgaagg ttctgagggc
     1501 cgtgctgggg ctcgagccgg ggctgcttgg cggggccacc cccccgttcc cggagtcgtg
     1561 ggagcagctc tgggaacagc tgacatcggc catgaggtgg gtggcctggc gtgcccatga
     1621 ggcggccctg gcgcgcgacg aggaggcggc acgtggggcg gggggccacc tccaagcatc
     1681 cctcgtggtg ggtgactgcg tcgagcgcgg caagggctac acccagggcg gcgcccggta
     1741 caaccactgc aactggaacg tcatcggcct cgccaacctc accgacgccc tcagcgccat
     1801 ccggtggctg gtgttcgacc aacgccagct cactctggcg gagctcgccg gcgtggtgag
     1861 cgccaactgg gtgggtcacg agccacttcg acagcgggtg ctcggagagt caccgaactt
     1921 cggcaacgac gatgaccggg cagacgccat cgcgaccgac ctgctgcgga tgatcgccgc
     1981 ggcctttgag ggcctcacgc catttcgagg tggcaagtac atcctcggca ctctcgcggg
     2041 tggggagaac atgcatatcg agttcggccg gatgacggga gcaacaccag acggccggcg
     2101 cgatggtgag ccgctggccg acagtgccgg gccggcgcag ggacgcgacc gccgcggtgt
     2161 gactgccatg atgaactcgg tggccaagct cccgcaccac ctgctgccga cggcgacgac
     2221 actcaatgtg aaactcgacc cgaggctgct cgaaaccgat acgggcctcg ccaaggtagc
     2281 tgggctgatc cgcgcgcact tcgccgccgg cggccagcag tgccagttca acatcgtgga
     2341 ccgcgagatg ctgctcgagg cccggcggga tccagagcag taccgcgatc tgatggtccg
     2401 cgtcgcgggc tacagcgcgc ccttcacttc gctgtgggaa gacctgcagg acgagatcat
     2461 cgcgcggacg gagcacgcgc tgtgagggga acaggaacca cgcaggggcg ctggccacat
     2521 gacgagcgag tggcgatggg actacgggtg gaatatgcgg acgcccgccc gggttaggcg
     2581 ggccacactc ggccaggcgc tcgcggcttt gggaggtctc atgttcgctc ttgcttgggc
     2641 tctgtgcgcg accggcgcct cgggcgccga ctgcttcgcc atccgcgtcg tcgatgaggc
     2701 cacgggccgc ggcgtcccgc tggtggccct acgcacggtc gacggaacgg tgcggtacac
     2761 cgacagtgcc ggcttcgtgg ccgtgcgcga ccccgagctc atgggccaac gggtcttctt
     2821 tcacatcagc agccacggct acgagtaccc ggctgatggc ttcggcaaca gggggattgc
     2881 gctagacgtg acgcccggcg gacgagccga agtgcgcgtc caccgggtga acatcgccga
     2941 gaggctgtac cggctgactg gagcgggtat ctaccgcgat agtgtcatcc tcggcgagcc
     3001 cgtccctgtc cgtcagccct tgctcaacgc cagggtcacg gggcaggact cggccctggc
     3061 catcccgtgg cgaggccgca tctactggtt cttcggcgac acgaaccgta tcgagtaccc
     3121 gctggggaac ttccacacgt ccggtgcgac ctcagccctg ccgggcgacg gcgggctgga
     3181 tccttcgatc ggggttgata tcgagtatct ggttgatgac gatggcttca gccgggccat
     3241 gtgccccatg gcaggcgagg ggcccgtgtg gctcgacggg ctggccgtgg cccgggaccg
     3301 tgacggcgcc gatcggctgg tggcccacta ctcccgggtg cgaggacttg agacgctcct
     3361 cgagcacggc ctcgccgtgt tcgacgacga gagacaagtg ttcaccaagc atgtcgagtt
     3421 cgcactcgac gacacacagc gctgtccacg gggccatccg gttgcgatac gcgaggacgg
     3481 gcgcgactgg ctcctcttcc cgacgccttt tgccagtctc cggtgtgagg caaccatcga
     3541 ggcactgtcg gaccccgggc gctatgagag cttcacgtgc ctcgcccccg gagcgcgctt
     3601 cgcggcggag cacaccgccg tcgagcgtgg ccccgatggc accctcgtat ggggatggaa
     3661 gccgaacacc gatgccatta cgcaggcaca ggaggccgag ctggcggtcg ccggcctctt
     3721 gaggccggac gagtgccgct atcaggtccg cgatggcacg gggcaggcca tccgcctgca
     3781 caacgggtcg ctcgcgtgga atgcctaccg ccagcgatgg atcatgatcg tggcacaggc
     3841 gggaggcagc agctcggtcc tcggcgagat ctggttcgcc gaagccgact cacccacggg
     3901 gccctggacg agctgcacga agatcgtgac ccatgaccac tacggcttgt acaatccggt
     3961 gcaccacccg tttctcgatc aggatggagg gcgggtgatc tacttccagg ggacatacag
     4021 ccagacgttt gaggggcccg tggcccccac gcccctgtat gactacaacc agatcatgta
     4081 ccggctcgac ctgtccgatg cccggttggg cccgccacca tcgcggcgct aggctcttcg
     4141 catcccaacg gaccgatccg cggagtctga ggaggccgat agttgctgct ccctctgatc
     4201 tgcagtgcgt ttcccgccac cgccctggcg ttgggggcct ctgagcgcca ggcggaggtg
     4261 accatccatc gcgaccaa
//
LOCUS       DAOSPL010000171         4273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20695_length_4273_cov_3.207681, whole genome shotgun sequence.
ACCESSION   DAOSPL010000171 DAOSPL010000000
VERSION     DAOSPL010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20695_length_4273_cov_3.207681"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="PLQ54_04425"
     CDS             <1..1178
                     /locus_tag="PLQ54_04425"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOS92534.1"
                     /translation="PQRIRALEEQGRWRMGQVIDRYRDYFGCLDYLHIFDFGESRVYG
                     RVGDYSHYDELGGLEAMRHAIAEAQDQGVRVGVYIEGYLCDERGVWGREHVAANEIKQ
                     ADGTGLLWAPGSTEHMMCAAAKGWRSHLAETYQRVARELGPDGMYIDQYGFTDAGKTC
                     WSREHGHPVPAAPIAGERDTLKAIRAATPPDVATLTEETPNDINSQYQDGALGYSVWN
                     ADADLAPHRIDLFRFAFPSFKVFQLVTYEPYLEGGWHLLKYPFFNGEGYWLAGGTTET
                     YCEEGHQFLKKAFAILHEYADAFTSDDVEPLVPTLEPTVYANRFRAGRKTVWTLMNGR
                     HRTFRGDVLRVPAIPGTQYREAFADRQLTPRVENGWAVISLELGPRAVGCVVARPAN"
     gene            1191..3320
                     /locus_tag="PLQ54_04430"
     CDS             1191..3320
                     /locus_tag="PLQ54_04430"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOS92535.1"
                     /translation="MRCFWPAMAAMAVLLPAAAWSGDVTVRRDDWGVPHIFARTPADA
                     AYGLGWAQAEDRLEQLLTNYRLAAGTMAEAFGPDYVEHDFQQRLVGNEEVCRRLYPSL
                     PRELRRMSESFIAGVKAYMAAHPEQVPPWAPDPEPWMPAAFGRLIILNWPLGQAKDEL
                     DRRAEVDPGFADAGAPGSPIRLAHLPMAGPFASNEWAVRPERTADGAAILLIDPHIPW
                     DGPFRFYEFRAHAGDFQVSGFAPLGVPLIGLGHNARLGWACTTGGPDTTDIYVEQLNP
                     DNPLQYRYDGEWRDAQVRTVEIQVRGGEPVRREIISTHHGPVILKEGNRAYAMACPYL
                     TEIGLVEQSYRMNFARNLQEFQDALAMCQFMEQNIMYADVEGNIYYCRTGRVPVRPSG
                     FDFSKPVPGNTSRSEWQGLHPLSALVQVLNPPQGFMQNCNISPDTMTGSGPVRASDYP
                     SWVFGDTPGRSNSRGRRAVELLSADDCVTQEDAVAIALDTHAEGAEAWQKALRQAAQR
                     AWDDPSVVRLRPALAILAAWDGHMDADSEGATLYRCLREAARRHDPPVDGEAIGQGHE
                     LDGTQQQALLNSLAAAVRQLEKDFGRLRVPWGQVHRVRRGDRSWPAAGGESGGGMTLR
                     AVGTTRDGAIYYGRSGQNWTQLVFFRRSGVESFSANPYGQSDRPDSAHYTDQAERLFS
                     RCHLKPTWFRPGALTGHVRAEQVLTAP"
     gene            complement(3448..>4273)
                     /locus_tag="PLQ54_04435"
     CDS             complement(3448..>4273)
                     /locus_tag="PLQ54_04435"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOS92536.1"
                     /translation="MGPHTAGNPAPVVDRSTGTVWLALNHNYGDRGENVIMEGKAPRT
                     VWLTHSDDDGVTWAEPTEITAQTKRPHWRWYATGPGHGIQLRDGTLLIPCDHSDHTDA
                     NHPYRSHVIVSRDHGATWQIGGVVGDGVNECMAAELADGTLYLNMRSYHGEHRRAVAR
                     STDGGRTWSAPELDSALIEPVCQASVLHVSLAPADVLLFCNPASTERERLTVRASLDG
                     GRTWPVSRLLWPGPAAYSDLAALPNGSIACLSEQGDGTAYERIRLQVFGLEWAVGT"
BASE COUNT          699 a         1494 c         1417 g          663 t
ORIGIN      
        1 cgcctcagcg catacgtgca ttggaagagc agggccgatg gcgaatgggt caggtcatcg
       61 atcgctaccg agactacttc gggtgcctcg actacctgca catcttcgac ttcggggaat
      121 cccgggtcta cggccgcgtg ggcgactaca gccactacga cgagctcggc ggcctcgaag
      181 ccatgcgcca tgccatcgcc gaggcgcaag accagggagt ccgcgtcggg gtctacatcg
      241 agggctacct ctgcgacgag cggggtgtct ggggccgcga gcacgtggcc gccaacgaga
      301 tcaagcaggc tgacgggacc ggcctgctct gggcgccggg atccaccgag cacatgatgt
      361 gcgccgccgc caaggggtgg cgttcgcact tggccgagac ataccagcga gtcgcccgcg
      421 agctcgggcc cgacggcatg tacatcgacc agtatggctt caccgatgcg gggaagacgt
      481 gctggtcgcg cgagcacggc cacccggtgc cggcggcacc catcgcgggc gagcgcgaca
      541 ccctcaaagc catccgggcc gccaccccgc cggacgtcgc cacgctcacc gaggagacgc
      601 ccaacgacat caactcccag taccaggatg gcgccctcgg ctactcggtg tggaacgccg
      661 atgccgacct cgcgccgcac cgcatcgacc tgttccgctt cgcgttcccc tcgttcaagg
      721 tcttccagct cgtgacctac gagccgtatc tcgagggcgg gtggcatctg ctcaagtatc
      781 cgttcttcaa cggcgaaggc tactggcttg ccggcggaac caccgagact tactgcgagg
      841 agggccacca gttcctcaag aaggcattcg ccatcctcca cgagtacgcc gacgcgttca
      901 cctctgacga cgttgagcca ctggtgccca ccctggagcc tacggtctat gcgaaccgtt
      961 tccgcgccgg ccgaaagacg gtgtggaccc tgatgaacgg gcgacaccgg accttccgcg
     1021 gtgatgtgtt gcgtgtgccc gccatacctg gtacgcagta ccgggaggcg ttcgccgatc
     1081 gccaactgac cccgcgggtc gagaacggct gggccgtcat ctcgctggag ctgggcccgc
     1141 gggccgtagg ctgcgtcgtc gcgcggccag cgaactgagg ggagctacgc atgcgctgct
     1201 tctggccagc catggccgcc atggcggttc ttctgcctgc cgccgcgtgg tcgggcgacg
     1261 tgactgtcag gcgcgatgac tggggcgtgc cgcatatctt cgcgcggacg ccggcggatg
     1321 cggcctacgg cctgggctgg gcccaagctg aggaccgtct cgagcagttg ctgaccaact
     1381 accgcctggc ggcggggacc atggccgagg ccttcgggcc cgactacgtc gagcacgact
     1441 tccagcaacg actcgttggc aatgaggagg tctgcaggcg gctgtacccc tcactaccgc
     1501 gggagctccg ccgcatgtcc gagtcgttca tcgccggagt caaagcgtac atggcagcac
     1561 accccgaaca ggtgccgccc tgggcacccg atcctgagcc ttggatgccc gcggccttcg
     1621 ggagactcat cattctcaac tggccgttgg gtcaggccaa ggacgagctg gaccggcgag
     1681 ccgaggtcga ccccggcttc gccgatgccg gggcgcccgg gtcacccatc cgcctcgcgc
     1741 acctgccgat ggccggcccg ttcgcctcca acgagtgggc cgtgcgcccc gagcggacgg
     1801 ccgatggcgc ggccattctg ctcatcgacc cccatatccc ctgggatggc cccttccgct
     1861 tctacgagtt ccgcgcccac gcgggggact tccaggtctc gggcttcgcg ccactcgggg
     1921 tgcccctcat cggactcggc cacaacgccc gtctgggttg ggcctgcacc acgggcggac
     1981 cggacaccac cgacatctat gtcgagcagc tgaacccgga caatccactg cagtaccgct
     2041 acgacggcga gtggcgcgac gcccaggtcc ggaccgtgga gatccaggtt cgtggcggcg
     2101 agcccgtccg gcgagagatc atctcgaccc atcacggacc ggtcatcttg aaagagggca
     2161 accgcgccta tgccatggcg tgcccgtacc tcacggagat cggcctggtg gagcagtcct
     2221 accgcatgaa cttcgcccgc aacctgcaag agttccagga cgcgctggcc atgtgccagt
     2281 tcatggagca gaacatcatg tacgccgatg tcgaaggtaa catctactac tgccggacgg
     2341 gtcgcgtgcc cgtgcgcccc agcggcttcg acttcagcaa gcccgtgcca gggaacacaa
     2401 gccgcagcga gtggcagggg ctgcacccct tgtccgccct ggtccaggtg ctgaatccgc
     2461 cccagggctt catgcagaac tgcaacatca gccccgacac catgacgggc agcggacccg
     2521 tccgcgcgag cgactatccc tcgtgggttt tcggcgacac tccgggccgc tccaactccc
     2581 gcggtcgccg cgctgtggag ctgctcagcg ccgatgactg cgtgacccag gaggacgccg
     2641 tcgccatcgc gctcgacact cacgccgagg gcgccgaggc gtggcagaag gcgctgcggc
     2701 aggcagcgca gcgcgcgtgg gatgacccat ccgttgtgcg cttgcgcccg gcgttggcca
     2761 tcctcgccgc gtgggacggc cacatggatg cggacagcga gggcgcgaca ctctaccgct
     2821 gtctgcgcga ggccgctcgg cgtcacgatc caccggtaga tggcgaggcc atcggccagg
     2881 gacacgagct cgacggaact cagcagcagg ccctgctcaa ctccctggcg gccgccgtgc
     2941 ggcagctcga gaaggacttc ggtcggcttc gcgtgccctg gggccaggtc cacagggtgc
     3001 gccgcgggga ccgttcgtgg ccggcggcgg gcggcgagtc cggcggcggg atgaccttgc
     3061 gcgccgtggg caccacgcgc gatggcgcca tctactatgg tcgctccggc cagaactgga
     3121 cacagctggt cttcttccgc aggtcggggg tagaatcgtt cagcgccaat ccctacgggc
     3181 agagcgaccg gcccgactcg gcacactaca ccgaccaggc cgagcggctg ttctcgcgct
     3241 gccacctgaa gccgacgtgg ttccggcccg gcgccctgac gggccacgtg cgggccgagc
     3301 aggtgctgac ggctccgtag cgcaggggcc tgcgaggtcg acggctacgc gcggccgtcc
     3361 tacaccatgc aaggaggcca cgggccagcg ctcacacgct cggtccgccc atcctgggaa
     3421 tgcggcaggc cgaacgccga tggcggccta cgtccccacc gcccactcga gcccgaagac
     3481 ctgcagccga atgcgctcat aggcagtgcc atccccctgc tcagataggc acgcaatgct
     3541 gccgttgggc aacgctgcca ggtccgaata cgccgccggg ccgggccaca ggagcctcga
     3601 gaccggccag gtgcgtccac cgtcgagact ggcgcggacc gtcaggcgct cccgctcggt
     3661 cgaggccggg ttgcagaaga gcagcacatc agcgggcgcg agcgacacgt ggagcacgct
     3721 cgcctggcac accggctcga tcagggccga gtcgagctcc ggcgctgacc aagttcggcc
     3781 tccatccgta ctacgcgcca ccgcacgccg gtgctcgcca tgatagctgc gcatgttgag
     3841 gtagagcgtg ccgtcggcaa gctcggctgc catacactcg ttgacaccgt cgcccacaac
     3901 gccgccgatc tgccaggtgg caccatgatc gcggctgacg atcacgtgtg aacggtacgg
     3961 atgattcgcg tcagtgtggt cggagtgatc gcaggggatc agcagggtgc cgtcgcgtag
     4021 ctggatcccg tgtcccgggc cggtggcata ccaacgccaa tggggccgct tcgtctgggc
     4081 ggtgatctcc gtcggctccg cccaggtgac cccgtcgtcg tcgctgtgcg tcagccatac
     4141 agtgcgcggc gccttcccct ccatgatgac attctcgccg cggtcaccgt agttgtggtt
     4201 cagagccagc cagaccgtgc ctgtggaccg gtcgaccacc ggtgcggggt taccggccgt
     4261 gtgggggccc ata
//
LOCUS       DAOSPL010000172         4272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20705_length_4272_cov_3.430401, whole genome shotgun sequence.
ACCESSION   DAOSPL010000172 DAOSPL010000000
VERSION     DAOSPL010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20705_length_4272_cov_3.430401"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLQ54_04440"
     CDS             complement(<1..245)
                     /locus_tag="PLQ54_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS92537.1"
                     /translation="MSKRILVVEDDRQARETLVFALEHEGYEVIEAADGEEALAKLAA
                     SKPDLVLLDLMLPRVDGLDVCKRAREMSSVPIIMLTAR"
     gene            complement(317..1225)
                     /locus_tag="PLQ54_04445"
     CDS             complement(317..1225)
                     /locus_tag="PLQ54_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOS92538.1"
                     /translation="MGQVRGTGKRVWVRRLALAVVLLGGWLASGSQARAVPGVRHVIV
                     VSVDGLRPDVLRAAAAPNVAALMGVGSWTLSARTVNPSVTLPAHCSMLSGVTPQVHGV
                     TRNDWSPGDGALGVSTAFSIARQYGLVCAAFVSKPKLRALAPQGVCDVFEVVPSDARA
                     VAARAAAYIERRRPNLCFVHLSDVDSAGHSAGWGSAAQVAACGQCDAAIGLLLGAAHR
                     AQIATESVMIVTSDHGGHGRGHGATSVADVTIPWVCAGRGVAVRYAIPTPVSVCDTAA
                     VALFGLGIARPADWTGRVVPGLFPGY"
     gene            1370..2443
                     /locus_tag="PLQ54_04450"
     CDS             1370..2443
                     /locus_tag="PLQ54_04450"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS92539.1"
                     /translation="MAKILKVGVIGLGGIARGAHIPGYKALDNVTLVAGADVDPKARK
                     AAREECGIPDVYDSYAEMLEKHPDLDLVSVCTGNRMHKDPTIAALKAGCHVLVEKPIA
                     MNAQDGQAMVDAAKKYGRRLMVGVNCRFDAKSQALKRLIDDGALGEVYAAKAIATRRR
                     GIPSWGVFSQKAESGGGPLIDIGVHCLDLAMYLMGYPKPVAVSGATYAKFGKRPPEDM
                     IDWRWNPRKFDVEDYASAFVRLENHACLTLEASWASNIDHDVFNVEILGDKGGCTWNP
                     CKLYSSRAGVLINLEPVRMPEVRSHSEEIRLYVDAILNDKETPAPAEQVLETQRILDG
                     IYASSEKNQEVWLRKPPATRKKR"
     gene            complement(2519..2995)
                     /locus_tag="PLQ54_04455"
     CDS             complement(2519..2995)
                     /locus_tag="PLQ54_04455"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS92540.1"
                     /translation="MEDREWWIRPYDGREDRAAVLSFQRETYELNFPDFVWSTSFIRD
                     FERILKMAERDPSEGLFVCEDATGRTAGFVWSSVSMRRFEGGQSVCTIKDMYVVPDLR
                     GQGVGMALMQAAEDFARRWKASRMALEVTVANRAAVRLYEKAGFTPTRYQMEKRLD"
     gene            complement(3140..>4272)
                     /gene="xylA"
                     /locus_tag="PLQ54_04460"
     CDS             complement(3140..>4272)
                     /gene="xylA"
                     /locus_tag="PLQ54_04460"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOS92541.1"
                     /translation="ATMLRPWDDGSNSLENAKNRVRAAFEFFEKLGVPFYAFHDRDVA
                     PEGESLAETNRNLDAVVEVMKEEQERTGVRLLWGTACLFSHPRFMHGASTSCNADAFA
                     YAAAQVKKALEVTHYLGGEGYVFWGGREGYTTHTNTDLKRENDHLGAFLRMAVDYKKK
                     IGFSGQFYIEPKPKEPTKHQYDSDAAACLAFLQHYGLLGDFKLNLETNHALLAGHTMA
                     HELEVALAYDALGSIDANDGDCMLGWDTDQFPTNIYLTTQIMLAVLRKGGLAPGGLNF
                     DAKVRRESFEPIDLFYAHIGGMDAFARGLKIAAAMRRDRLFEKIIADRYASWDEGIGR
                     QIESGQADFESLERYMLAKGDIAPNASGRQELIENWINDYIR"
BASE COUNT          787 a         1492 c         1314 g          679 t
ORIGIN      
        1 cgcgcggtga gcatgatgat gggcaccgaa ctcatctcac gggcgcgctt gcacacgtct
       61 agcccgtcca cgcggggcag catgaggtcc aggagcacca gatcgggctt ggacgcggca
      121 agcttggcga gcgcctcctc cccgtccgcc gcctcgatga cctcgtaccc ttcgtgctcg
      181 agggcaaaga ccagggtctc tcgagcttgc cgatcgtctt cgacaacgag gatccgcttg
      241 ctcatcgcag cccaccgtcc gctcatctgc ggtcggcacg ggagcaccgg cccgatcgct
      301 gaggtcgccc cgtgccctag taacccggga acagcccggg caccactcgt cctgtccagt
      361 ctgccgggcg ggcaatgccg agcccaaaca gcgccaccgc ggccgtatca cacacactca
      421 ccggagtcgg gatggcgtag cgcaccgcaa ctccacgccc cgcacacacc cacgggatgg
      481 tcacatcggc caccgacgtg gccccatggc cccggccgtg ccctccatgg tccgatgtga
      541 cgatcatcac gctctcggtc gcgatctgag cccggtgagc ggcccccagc agcagtccaa
      601 tggccgcgtc acactgcccg cacgccgcaa cctgtgcggc gctcccccag cctgccgaat
      661 gccctgccga gtcgacgtcg ctcaagtgca cgaagcacag gttcggtcga cgccgctcga
      721 tataagccgc agcccgagcc gccaccgcgc gcgcatcgct cggcaccacc tcgaacacgt
      781 cgcagacccc ctgcggcgcc agcgcgcgga gcttgggttt gctcacgaac gccgcacaca
      841 ccaggccgta ctgcctggca atgctgaatg cggtcgacac gccgagcgcc ccatctccgg
      901 ggctccagtc attccgggtg acaccatgaa cctggggcgt cacccccgag agcatcgagc
      961 aatgcgctgg cagagtcacg ctcgggttca cggttcgtgc tgagagggtc caggaaccca
     1021 cgcccatcag ggccgccaca ttgggcgccg cggccgctcg cagcacgtca ggccggaggc
     1081 catcgacgct cacgacgatc acgtggcgaa cgcccggcac ggccctcgct tgggagcccg
     1141 aggcgagcca cccgccgagg agcaccactg caagagcgag acgccggacc cacacacgct
     1201 tgccggtacc ccgaacctga cccatagcta gcgtttacat tctcggccat ggggcggact
     1261 cctgccaccc tggcgcgcgg tttcgggttc cacggaggat tccgtctgcc gggatccgta
     1321 cacagcggtg gtcccgagag tgggagccca cggaaggaga tggactggct tggccaagat
     1381 tctgaaggtc ggcgtgatcg gccttggcgg gattgcccgc ggggcccaca tccccggata
     1441 caaggcgctg gacaacgtca cactcgtcgc cggagccgat gtcgacccca aggcccgcaa
     1501 ggcggcgcgc gaggagtgcg gaatccccga cgtctacgac agctatgccg agatgctcga
     1561 gaaacacccg gacctggacc tggtgtcggt gtgcacgggc aaccgcatgc acaaggaccc
     1621 caccatcgcc gcgctcaaag cgggctgcca tgtgctggtc gagaaaccca tcgccatgaa
     1681 cgcccaggac ggccaggcga tggtcgacgc ggcgaagaag tacggacggc ggctgatggt
     1741 gggggtcaac tgccgtttcg acgccaagtc gcaggcactc aagcgcctca tcgacgatgg
     1801 ggccctcggc gaagtctacg cggccaaggc gatcgccacc cgccgtcgcg gcatcccgag
     1861 ctggggcgtg ttctcgcaga aagccgagtc gggtggcggg ccgctcatcg acattggcgt
     1921 ccactgcctc gatctggcca tgtacctgat gggctatccc aagcccgtcg ctgtcagcgg
     1981 cgccacctat gccaagttcg gcaagcgccc ccccgaggac atgatcgact ggcgctggaa
     2041 cccgcgcaag ttcgatgtcg aggactatgc ctccgcattc gtccgcctcg agaaccacgc
     2101 atgcctcacc ctagaggcca gttgggcctc caacatcgac cacgacgtgt tcaacgtcga
     2161 gatcctcggc gacaagggtg gatgcacctg gaacccatgc aagctgtact cgtctcgggc
     2221 cggtgtgttg atcaacctcg aacccgtgcg aatgcctgag gtgcggagcc actccgagga
     2281 gatccgcctc tacgtcgatg ccatcctcaa tgacaaggag actcccgcac cggccgagca
     2341 ggttctcgag acccagagga tcctcgacgg catctacgca tcgagcgaga agaaccagga
     2401 agtctggttg cgcaaaccgc ctgccacccg gaagaagcgt tagccgacag caggaaccca
     2461 cacatggcca tggcggagcg agggagccgc tcggcgcagt ccccctcgct ccgctgccct
     2521 aatcgaggcg cttctccatc tggtagcgag tgggcgtgaa gcccgccttc tcatagaggc
     2581 gcacggcggc ccggttcgca acagtgacct cgagagccat gcggctggcc ttccaccgcc
     2641 gggcgaagtc ctcggcggcc tgcatgagcg ccatgcccac gccctggccg cgcaggtcgg
     2701 gcaccacgta catgtcctta atggtgcaca cggactgtcc gccctcgaac cgtcgcatgc
     2761 tgacgctgga ccacacgaat cccgcggtcc gccccgttgc gtcctcacag acgaacaaac
     2821 cctcactcgg gtcacgttca gccatcttga ggatgcgctc gaaatcgcgg atgaaggacg
     2881 tcgaccagac aaagtccggg aagttcaact cgtaggtctc gcgctgaaaa ctcagaacgg
     2941 ccgcgcggtc ctcacggccg tcgtaggggc ggatccacca ctcgcgatcc tccatcgcac
     3001 cctccccaac gcagcaccgc aggggaccca ctcgcggacg cgcaatccgc ggcctcgacc
     3061 gcccgggtga tgcccaaagt cggcgcccca ggtcgccttc gccggccaaa tgctccgtac
     3121 cgcagcggcc agccgatacc tagcggatgt agtcgttgat ccagttctcg ataagctcct
     3181 ggcggccgct ggcgttgggg gcgatatcgc ccttggccag catatagcgt tcgaggctct
     3241 cgaagtccgc ctgacccgac tcgatctgcc ggccgatgcc ctcatcccac gacgcatagc
     3301 ggtcggcgat gatcttctcg aacagacggt cccgccgcat ggccgcggcg atcttgagcc
     3361 cgcgggcgaa agcatccatg cccccgatgt gggcatagaa gagatcgatg ggctcgaaac
     3421 tctcccggcg taccttggcg tcgaagttga gccctccggg cgccaggcca cccttgcgca
     3481 acacggcgag catgatctgg gtggtcaggt agatgttcgt cgggaactgg tcggtgtccc
     3541 atcccagcat gcagtcacca tcgttggcgt cgatggagcc cagggcgtcg taggccagtg
     3601 ccacctcaag ctcatgggcc atggtgtggc cggccaaaag ggcatggttg gtctcgaggt
     3661 tcagcttgaa gtcgccaagc aggccatagt gctgcaggaa ggccaggcac gcggcggcat
     3721 cgctatcgta ctggtgcttc gtcggctcct tgggcttggg ctcgatgtag aactggccgc
     3781 tgaagccgat cttcttcttg taatcgaccg ccatccgcag gaaggcccca aggtggtcgt
     3841 tctcccgctt caggtccgtg ttggtatgcg tggtgtagcc ctcacggccc ccccagaaca
     3901 cgtagccctc gccgcccaga tagtgggtga cctccagggc cttcttcacc tgggcagccg
     3961 cgtaggcgaa ggcatcggca ttgcagctcg tcgaggcccc gtgcatgaac cgaggatgcg
     4021 agaagaggca ggccgtgccc cagagcagtc ggaccccggt gcgctcctgc tcctccttca
     4081 tgacctcgac cacggcatcg agattgcggt tggtctcggc caacgactcg ccctcaggtg
     4141 ccacgtcgcg atcgtgaaag gcgtagaacg gcacgcccag cttctcgaag aactcgaacg
     4201 cggcgcgaac acgattcttg gcgttctcga gcgagttcga gccgtcatcc cacggccgca
     4261 acatcgtggc at
//
LOCUS       DAOSPL010000173         4270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20719_length_4270_cov_3.467616, whole genome shotgun sequence.
ACCESSION   DAOSPL010000173 DAOSPL010000000
VERSION     DAOSPL010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20719_length_4270_cov_3.467616"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLQ54_04465"
     CDS             <1..336
                     /locus_tag="PLQ54_04465"
                     /inference="COORDINATES: protein motif:HMM:NF036850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOS92542.1"
                     /translation="LRGCSTEPKDLHPEVADADMVVNCTPLGMSPRADAMPPVDTDWL
                     RPETVVCDLIYNPPTTRFLQSARARGCRTIDGRGMLVHQGALSLAMWTGFAPPTDTMR
                     EALDQQLGV"
     gene            435..1223
                     /locus_tag="PLQ54_04470"
     CDS             435..1223
                     /locus_tag="PLQ54_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92543.1"
                     /translation="MVPRPQASVMVARIGLLAVALLPAAGRTLHADDARSLGELVARA
                     RCVCIGTVIGDEPLDGQRRLTLNPVTALKGATASPLDVAVPQSQGQQDLPDQLVHRQL
                     IAFLGERNECIARYAVSPDGTVIAPDFLPKNRESITVDESRRSIKEIVKLREAIDAQP
                     EATPGACALASRSDLDLVRLYAAAVLDAHRAARPGLEAGQHGAPVPAEDTNDAQNGSG
                     GAGYTLATLGISALALLSFILVFRKCWREAGSAEAGSPTPARDR"
     gene            1230..1784
                     /locus_tag="PLQ54_04475"
     CDS             1230..1784
                     /locus_tag="PLQ54_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOS92544.1"
                     /translation="MPSERRADKIARVLASRQHDLHVVLDDIYHPHNISALLRTCDGA
                     GVGCIHIIGETLADGVSAAVSRGCERWSEIEYHDNIAECYARLHWSGFRVWCTGAGRG
                     SIDYRAVDLTQKVAIVLGNERRGASGAARALADGLIWIPMLGMVESLNVSVAGGVILF
                     EALRQRGLPPVGQPVDSLADNRQR"
     gene            1887..3248
                     /locus_tag="PLQ54_04480"
     CDS             1887..3248
                     /locus_tag="PLQ54_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HOS92545.1"
                     /translation="MAVQDVELLVLDTPPQQFCDTLTQLRSEWRSRLDRGDGDALDEI
                     REVTLDLESYLPKWNLLNPAREPISASDLAACIEGNYELLRSAGSLAADAVAEEAARL
                     RASNLLRLARLADEGVINARWGNDYASGLKDAMRRGAIVVTTNPQLVNIARKEHPAEW
                     APVRDRLRAAHRDPEELAKAMTMEVVLKNARLLRPVYRATGGAMGYVSFQLNPKKADD
                     ADAMISDAQTIFAELRGRFEDGEPNVVFKVPGTFAGLRVAEALTAQGIGINVTVNYAV
                     PQQLAFGEAIEKGNARHSYLTNMDGRLDDPVGAELGEGKADHPDKASSWAGIAVAKKA
                     HKLLYQTLGYRKSILLDASGRGPWHITRSLPGPDDYPVHKTVFPDKQAEFDASEVQIQ
                     PTMREEVPKDALDKLYGSKLFRQAYEVNGLHPLEFDAFEPAVDTLRSFSAQYDEFVSW
                     CEG"
     gene            3345..>4270
                     /locus_tag="PLQ54_04485"
     CDS             3345..>4270
                     /locus_tag="PLQ54_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS92546.1"
                     /translation="MIRLGVMGFGGRISSMINHVFREVEPDIRVVGIVDPDEEGARSR
                     LAECDKADAVFYTSTDEMVRQAKPDALAVGTRCNLHTPYAVEAARYDLPLFLEKPVAT
                     SMEQATALEDAFAASRCQAVVSFPLRVSPLCVLTRQYIDEGAVGPAEHILGVNYVPYG
                     TCYFDGHYRNFAVTQGLFLQKATHDFDYMSYLMGSTIIRVAATAQYGRVFGAPGARGK
                     GMPAGLVCSQCDDALVCLESPENRKRNGSGGTDQDHPCVFGVDCGSPEAGMNEDASNA
                     LLQFANGAAGVYTQVFYSRRDSGTRGSTISGY"
BASE COUNT          719 a         1434 c         1396 g          721 t
ORIGIN      
        1 ctgcggggct gttccacaga gcccaaagac ctgcatcccg aggttgctga tgccgacatg
       61 gtggtcaact gcaccccact ggggatgagc ccccgggccg atgccatgcc acccgtcgac
      121 accgactggc tccgccccga gaccgtggtg tgcgatctga tctataatcc acccacaacc
      181 cggtttctcc agagcgctcg cgcgcgcggg tgccgaacca tcgatggtcg aggcatgctc
      241 gtccatcagg gcgcgctctc gctggccatg tggacggggt tcgccccgcc aaccgacacc
      301 atgcgcgagg cgctcgacca gcagttgggg gtctgacacg ttctgggccg tggtcgccgg
      361 cgggtccgaa ggccattgcg cggcgcaaag acgccggtcc acgtggtacg ggagtgaggg
      421 gcgaagggag cggaatggtg ccccgtcccc aggctagtgt catggtcgcg cggatcggtc
      481 tgttggcggt ggctctcttg cccgccgccg gccggactct gcatgccgac gacgctcgca
      541 gcctcggcga gctcgtcgcg cgagcgcgct gcgtctgcat cggcaccgtc attggcgatg
      601 agcctttgga cggccagcgc cgtctgaccc tgaacccggt aaccgccctc aagggggcca
      661 ccgccagccc cctggacgtc gcggtacccc agtcgcaggg ccagcaggac ctgcctgacc
      721 agctggtcca tcggcagctc atcgccttcc tgggtgagcg caacgaatgc atcgcccgct
      781 atgctgtcag ccccgatggc accgtcatcg cccccgactt cctgccgaag aaccgcgaga
      841 gcatcacagt cgatgagtcc cgccggagca tcaaggagat cgtcaaactt cgtgaggcca
      901 tcgatgccca gccggaagca acccctggcg cgtgtgctct ggcatcccgg agcgacctcg
      961 atctggtgcg cctctatgcg gcggctgtgc tagacgcgca ccgtgcggct cgccccgggt
     1021 tggaggcggg tcagcatggc gctccagtac cggctgagga cacaaacgac gcgcagaatg
     1081 gctcgggtgg cgcgggctac acactggcaa ctctcggcat ctcggccctg gccctgttgt
     1141 ccttcattct ggtcttccgc aagtgctggc gcgaggccgg gtcagccgag gccggaagcc
     1201 ccacgcccgc gcgggaccgg tgaggctcca tgccgtccga gcgccgggcg gacaagatcg
     1261 cccgggtcct cgcttctcgc cagcacgacc tccacgttgt gctcgacgac atctaccacc
     1321 cgcacaacat cagcgcgctg ctccgcacgt gcgacggggc gggcgtgggc tgtatccaca
     1381 tcatcggcga gacgctggcc gacggcgtgt ctgccgcggt gtcccgcgga tgcgaacgat
     1441 ggtccgagat cgagtaccac gacaacatag cggagtgcta tgctcgtctt cactggtccg
     1501 gtttccgcgt ctggtgcacg ggtgccgggc ggggctcgat cgactaccga gctgtggacc
     1561 tgacccagaa ggttgccatc gtgctgggga atgagcgccg aggtgcatcg ggcgccgccc
     1621 gggcgttggc tgatggcctg atctggattc cgatgctggg catggttgag agcctgaacg
     1681 tctccgtggc cggaggcgtg atcctgttcg aggccctgcg tcagcgaggt ctgcctccgg
     1741 tcggccagcc tgtggactcc cttgccgaca accggcagcg atagcaaacg gatgtttggt
     1801 tatgcctccc tggtgggtag gagtaagatg ggcgccggcg tggaacgccc gacccgcgcc
     1861 gaccgctggc gaatgaaggt ggacccgtgg ctgtgcagga cgtggaactc ctcgtactcg
     1921 atacccctcc gcaacagttc tgtgataccc tcacccagct ccggtccgaa tggcgctcac
     1981 gcctcgaccg tggcgacggc gatgccctcg acgagatccg tgaggttacc ctcgatctcg
     2041 agtcgtacct ccccaagtgg aatctgctga accccgcccg ggagccgatc agcgctagtg
     2101 acctggcggc gtgcatcgag ggcaactacg aactcctccg gtccgctggc agtctcgcgg
     2161 ccgacgcggt ggctgaagaa gccgcgcggc tccgcgcgag taacctgttg cgtctggccc
     2221 ggctcgccga tgagggggtg atcaacgccc gatggggcaa cgactacgcc tccggcctca
     2281 aggatgccat gcggcgcgga gccatcgtgg tcacgaccaa cccccagctc gtcaacatcg
     2341 ctcgcaagga gcaccccgcc gagtgggccc cggtgcgcga ccggctccgc gcggcgcacc
     2401 gtgatcccga agagttggcc aaggccatga ccatggaggt cgtgctcaag aacgcgcggc
     2461 tcctccggcc agtgtaccgc gccaccggcg gagccatggg atacgtgagc ttccagctca
     2521 acccgaagaa ggcggacgat gccgatgcga tgatctccga tgcccagacc attttcgcgg
     2581 agctgcgcgg ccgctttgag gacggtgagc ccaacgtggt gttcaaagtt cccggcacct
     2641 ttgcgggctt gcgggtggcc gaggcgctca cggcccaggg cattggcatc aacgtcaccg
     2701 tgaactacgc ggtgccccag cagctcgcct tcggcgaggc catcgagaag ggcaacgccc
     2761 gccactccta cctgaccaac atggatggcc ggctcgacga tccggtgggt gccgagctgg
     2821 gcgaggggaa ggccgaccat cccgacaagg ccagcagctg ggccggtatc gcggttgcca
     2881 agaaggcgca caagctgcta taccaaacac tgggataccg gaagagtatc ctcctcgatg
     2941 cgtcgggtcg cggaccgtgg cacatcaccc ggtctctgcc cggtccggat gattacccgg
     3001 ttcacaagac tgtgttcccg gacaaacagg cggagttcga cgcgagcgag gtgcagatcc
     3061 aaccgacgat gcgggaagag gttcccaaag acgccctgga caagctctat gggagcaagc
     3121 tcttccggca ggcctacgag gtcaacgggc tccatccatt ggagttcgat gccttcgagc
     3181 ctgccgtcga tacgctgagg tcgttctccg cccagtatga cgagttcgtc agttggtgcg
     3241 agggatagct ccccggttcg ggtgcaaggc tgcgtggttc gggccttgca ccccccaccc
     3301 cccgcgactc cgtgtggttt cccgacccct gagaggtggc ctctgtgatc cggctaggtg
     3361 tgatgggctt cggcggccga atcagcagca tgatcaacca tgtgtttcgc gaggtggaac
     3421 ccgacattcg ggtcgtgggg atcgtcgatc ccgacgaaga aggcgcgcgg tcgcgtctcg
     3481 ccgagtgcga caaggctgac gccgtcttct acacatcaac cgacgagatg gtccgccagg
     3541 ccaagcccga tgccctggct gtcggcaccc gatgcaatct gcacacgccc tacgccgtcg
     3601 aggccgcccg ctacgacctg cccttgttcc tcgagaagcc cgtggcgacg tccatggagc
     3661 aggccacggc gctcgaggat gccttcgcgg catcgcgctg ccaggcggtg gtgagctttc
     3721 cgctccgggt ctcgccgctg tgcgtcctga cccgccagta catcgacgag ggcgccgtgg
     3781 gcccagcgga gcacatcctg ggcgtcaact acgtccccta tggcacctgc tacttcgacg
     3841 gccattaccg gaacttcgcg gtcacccaag ggctcttctt gcagaaggcg acacacgact
     3901 ttgactacat gagctatctg atgggctcga ccatcatccg cgtcgccgcg accgcccagt
     3961 acggccgggt cttcggcgcc ccgggagccc gcggcaaggg catgcctgcc ggcctggtgt
     4021 gcagccagtg cgacgatgcg ttggtctgcc tggagagccc ggaaaaccgg aagcggaacg
     4081 gttcgggcgg tacggaccag gatcacccgt gcgttttcgg cgtcgactgc ggaagtccgg
     4141 aagccgggat gaacgaagac gcctcgaatg ccctgcttca gttcgccaat ggcgccgccg
     4201 gagtctacac ccaggtcttc tactcgcgac gcgactcggg cacccgtggc tcgaccatca
     4261 gcggctacca
//
LOCUS       DAOSPL010000174         4269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20726_length_4269_cov_4.059563, whole genome shotgun sequence.
ACCESSION   DAOSPL010000174 DAOSPL010000000
VERSION     DAOSPL010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20726_length_4269_cov_4.059563"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            561..728
                     /locus_tag="PLQ54_04490"
     CDS             561..728
                     /locus_tag="PLQ54_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92547.1"
                     /translation="MRRLWVLAVLALAVLCAACEMQGNLQYANGDPVTSSGGSSGSGG
                     GGGTQPPAPPD"
     gene            complement(735..2237)
                     /gene="argH"
                     /locus_tag="PLQ54_04495"
     CDS             complement(735..2237)
                     /gene="argH"
                     /locus_tag="PLQ54_04495"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOS92548.1"
                     /translation="MSKGLLWGGRFAAPPSARSLAFMSGRDVEPTPPYDLALAPYDLW
                     VNRAHSLMLGRTGIVPAASVRHILRALTTLEERLAAGEFALDPAKEDVHSNVEAMVTE
                     LVGIEHAGHMHTGRSRNDQVVTDMRLYLRDRALEAADAILDLVGALIAAARDHAMTLM
                     PGFTHHQHATATTWGHVTLAYAWAGLRQAQRLLAFLGTWNRCPLGSAASYGTTLPINR
                     GLTAELLGFDGPEPNTLDSINSRWETAADLVHCLASMLTQLSSLSQTLILFSTREFRA
                     VRIPDEYSAGSSIMPQKRNPQALEAIKAHAVMAQGAASTLMSLGGHNLFGYNTDQMWT
                     KYVALDAIGGSVASIRAMAGVIAGLEVNVERLRELASRDFLGSTALAEALVSDGGVPF
                     RIAKQVVEKAVALSEQSGEEQVTEEALRCSLAERGVEVDADLCTLVESQRAEAAVARL
                     LHAGGAAPERVLESCGEAEGLRAALAVEVAGRAQAVRVARAACREACDAF"
     gene            complement(2294..3478)
                     /locus_tag="PLQ54_04500"
     CDS             complement(2294..3478)
                     /locus_tag="PLQ54_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS92549.1"
                     /translation="MIDITRLLCGTVHIGDALRYADRSEAARPDMLRFALEKKPVVVW
                     NMTRQCNLSCVHCYAHAKPCADPDELTTREAQALIDDLGQFGAPVLLFSGGEPFVRPD
                     LYDLGRRAIEHGIRAVISTNGTLITEEAALRVKEVGFSYVGVSLDGGRNTNDRFRGQP
                     GAFDAALEGIRNCHAAGVKAGLRFTVNKRNVNDLPEILDLLEEERIVRCCIYHLVYAG
                     RGTRLMDEDLSPDETRRVMDLLMERAVDFHQRGLPTELLTVDNHADGVYLYLRLRERD
                     PERAEEVLTLLRRNGGNSSGVGIGDVDQRGDVHADQFWSHYSFGNVRQRPFSQIWTDV
                     SDPIMAGLKNRRPLLKGRCGACKWQDICNGNFRVRAEAVYGDVWAQDPACYMTDEEIG
                     IA"
     gene            complement(3475..4152)
                     /locus_tag="PLQ54_04505"
     CDS             complement(3475..4152)
                     /locus_tag="PLQ54_04505"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOS92550.1"
                     /translation="MRIAAPDDFGPLDAAIDRLPSYHVAVFTSANGVDRFVQRLEHHQ
                     LDIRALAGIELVCIGPKTAEAFTRRGIRVSAVPETFVAEALVELLSARDLRGKRILLA
                     RAAEAREVLPDSLRAAGAEVDVVPAYRTIRDDTDITEVVELFESGCVDAVTFASAGTV
                     SQFVGLLGEERARTYLGRTAVAVIGPITAQAVEALGLAAAIRARESTIESLVEAIAEY
                     FAGKRRP"
BASE COUNT          664 a         1444 c         1491 g          670 t
ORIGIN      
        1 gtccagcgaa ccatgttcgc attcgccgga gcagatagca atacctcctt actgacagat
       61 tgggggtgcg ggctgccgcg cccgccgccc ttcgtcacgc cgcgcgtcgc acgggaccct
      121 tgggccggat agggtcccac cagccttcgg ctggtccgcc cctattcggc ccctccgaaa
      181 ggcattggaa caaccgagga ggggccgaat ggaaggggcc cgcgcagcgg gaagggcgcc
      241 atccggcccg gtctccagag acccataccc ccaacttcgc ctcaatccct gtctactcat
      301 tggggtggac ttcgcggttc gcacaccccc cgtcggccct gagcctgtcg aagggaggag
      361 cgcgggtagt gctcccgctg cccttcgcca cggcgcgtga acgcggcggc tcagggtcgg
      421 cgggttggga ggcgtgcggc ggtcgatggt gggcgccgag ggcgcctacg cgattcccga
      481 gacacagtac ccgcttggca ccgtcggtaa aggaggcgta cgcggtcggg cgaaggatcg
      541 tgttgcaggg aggtggcgct atgcgtcgcc tgtgggttct ggcggttctg gcgctggcgg
      601 tgctgtgtgc cgcgtgcgag atgcaaggca acctgcagta tgcaaacggc gacccggtga
      661 cgtcgtcggg cggcagttcc ggctccggcg gcgggggcgg cacccagcca cccgcaccgc
      721 ctgactagcg gccgctagaa cgcatcacac gcctcgcggc aggcggcacg ggcaacgcgg
      781 acggcctggg cccgtccggc gacctcgacg gccagcgcgg cgcgaaggcc ttccgcctca
      841 ccgcagctct cgagcacgcg ctcgggcgcg gcgccgccgg cgtgcaggag ccgcgccacg
      901 gccgcttcag cgcgctgcga ctcgacgagc gtgcagaggt ctgcatcgac ctcgacgccc
      961 cgctcggcca gcgagcagcg gagggcctcc tcggtgacct gttcctcgcc cgactgctcg
     1021 gagagcgcca cggccttctc gaccacctgc ttggcgatgc ggaacggcac tccgccatcg
     1081 ctcaccagcg cctcggccag cgctgtcgag ccgaggaagt cccggctggc cagttcacgc
     1141 aggcgctcga cgttgacctc gagcccggcg atcacgccgg ccatcgcgcg aatggacgcg
     1201 acggacccgc cgatggcgtc cagggcgacg tacttggtcc acatctggtc ggtgttgtag
     1261 ccaaacaggt tgtgaccgcc gagggacatg agggtgctgg ccgcgccctg cgccatcacc
     1321 gcatgtgcct tgatggcctc cagcgcctgc gggttgcgct tctgcggcat gatcgagctg
     1381 cccgccgagt actcatcggg gatgcgcacg gcgcggaact cccgcgtcga aaacaggatc
     1441 agcgtctgcg atagcgacga gagctgcgtg agcatcgacg ccaggcagtg gaccagatcg
     1501 gccgccgtct cccatcggct gttgatgcta tcgagcgtgt tcggctccgg tccatcgaag
     1561 ccgagcagct cagccgtcag tcctcggttg atgggaagcg tggtcccata ggaggcggcg
     1621 ctgcccaaag ggcaacggtt ccacgtgccg aggaaggcca gcaaccgctg ggcctgacgc
     1681 aggccggccc aggcataggc caaggtcacg tggccccagg tggtcgctgt ggcgtgctgg
     1741 tggtgggtga agccgggcat gagggtcatc gcatggtcgc gcgccgccgc gatgagagcg
     1801 cccacgaggt cgaggatggc atcggcggcc tccagtgccc gatcgcgcag gtagaggcgc
     1861 atgtcggtga cgacctgatc gttccgcgag cgcccggtgt gcatgtgtcc ggcgtgctcg
     1921 atgccgacga gctcggtgac catggcctcg acgttcgagt ggacgtcttc tttggccggg
     1981 tcgagggcga actcgcccgc agccaggcgc tcctcgaggg tcgtcagggc ccggaggatg
     2041 tgccgcacgc tggcggcggg cacgatgccg gtccggccaa gcatgagcga atgggcgcgg
     2101 ttcacccaca ggtcataggg cgccagcgcg aggtcgtacg gcggcgtggg ctcgacatcg
     2161 cggcccgaca tgaatgccag gctccgcgcg ctcggcggtg ccgcgaagcg cccgccccag
     2221 agaaggccct tggacatgga tcggatgcac tccctgatgg tccgccgggt gacgggcggt
     2281 ctgccacggc accctacgcg atcccgatct cttcatcggt catatagcac gccgggtcct
     2341 gagcccagac gtcgccatag accgcctcgg cacggacgcg gaagttgccg ttgcagatgt
     2401 cctgccactt gcaggcgcca catcggccct tcagcagagg ccggcggttc ttgagccccg
     2461 ccatgatcgg gtccgagacg tcggtccaga tctgagagaa cggcctctgg cgcacattgc
     2521 cgaaggagta gtggctccag aactggtcgg cgtgcacatc gccccgctgg tcgacgtcgc
     2581 cgatcccgac gcccgacgag ttgccgccgt tccggcgcag gagagtcagc acctcctcgg
     2641 cccgctcggg gtctcgctcg cgcagccgga ggtagagata cacgccatcg gcatggttgt
     2701 cgacggtgag caactccgta ggcaggccgc gctggtggaa gtcgacggcg cgctccatga
     2761 gcaggtccat cacgcgccgt gtctcatcgg gcgagaggtc ctcgtccata agccgcgtgc
     2821 cgcggccggc gtagaccaga tggtagatgc agcagcggac gatgcgctct tcctcgagca
     2881 ggtcgaggat ctcgggaagg tcgttcacgt tgcgcttgtt aaccgtaaag cggaggccgg
     2941 ccttgactcc ggcggcgtgg cagttgcgga tgccctcgag ggcggcgtcg aatgcccccg
     3001 gctggccgcg gaagcggtcg ttggtattgc ggccaccatc gaggctcacg cccacgtagg
     3061 agaacccgac ctccttgact cgcagggcag cttcctcggt gatcagtgtt ccattcgtcg
     3121 agatgacggc ccggataccg tgttcgatgg cgcgtcggcc caggtcgtac aggtcgggcc
     3181 ggacgaaggg ctcgccgccg gagaacaaca gcaccggagc gccgaactgg cccagatcat
     3241 cgatgagggc ctgcgcctcg cgcgtggtga gttcatcggg atcggcacac ggcttggcat
     3301 gggcatagca gtggacgcac gagaggttgc actggcgcgt catgttccag acgacgaccg
     3361 gcttcttctc gagcgcgaac cgcagcatgt ccggccgcgc cgcttcgctg cggtcggcgt
     3421 atctgagggc gtcaccgatg tgcacggtgc cgcagagtag tctcgtgatg tcgatcatgg
     3481 tcgtctcttc cccgcgaagt actcggcgat ggcctccacg agcgactcga tggttgactc
     3541 tcgcgcccgg atcgccgccg ccagccccag agcctccacc gcctgagccg tgatggggcc
     3601 aatgacggcc acggccgtgc gccccaagta tgtccgagcg cgctcctcgc ccagcagtcc
     3661 cacgaactgg gacacggtgc cggcgctggc gaaggtcaca gcgtccacgc agccggactc
     3721 gaacagctcg acgacctcgg tgatgtctgt gtcgtcgcgt atggtcctgt aggcggggac
     3781 gacgtccacc tcggccccgg cggctcggag tgagtcaggc aggacctcgc gcgcctcggc
     3841 agcgcgtgcc aagaggattc gcttgcccct gaggtcgcga gccgacagca gctcgaccag
     3901 cgcctcagcc acgaatgtct cgggcacggc actcacgcgg atgccccggc gggtgaacgc
     3961 ctcggccgtc ttggggccga tgcagaccag ttcgatgccg gccagcgcgc ggatgtcgag
     4021 ctggtggtgc tcgaggcgct gcacgaaccg atcgacaccg ttcgcgctgg tgaacaccgc
     4081 gacgtggtag gacggcagcc ggtctatcgc cgcgtcgagg ggtccgaagt cgtccggtgc
     4141 cgcgatgcgc acacctaccc cgccggggct gaagcgctac gcgccgccgc cgccgcggcc
     4201 ggccccctcg tcaagctcat cgacgaaccc ggccgcgttc agcgcccgaa tcttggcctc
     4261 cgacgcgtc
//
LOCUS       DAOSPL010000175         4262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20781_length_4262_cov_3.124079, whole genome shotgun sequence.
ACCESSION   DAOSPL010000175 DAOSPL010000000
VERSION     DAOSPL010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20781_length_4262_cov_3.124079"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1961
                     /locus_tag="PLQ54_04510"
     CDS             <1..1961
                     /locus_tag="PLQ54_04510"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOS92551.1"
                     /translation="DVTTADVIDRTRELLGSLPRRALKAVARESEPPQTAPRRRTIVD
                     PDLDTAYLIMAYRSIDLLHPDLYALDVLSYVLSQGASSRLVRTLRDEQSLVSHIETHS
                     YTPPRRPGVFAVEAMVDPANLVPAEAAIADELLRCQTEEVTPEELDRAKAQKSAELVF
                     ARETVEGLASQLAFDYYATGDVRFSERYVEGIRRVTAEDVKRVAATYLRPEALCVALL
                     TPTESEASQEAVGAPSAAPVQKVTLDNGLTLLLGEDHAVPMVSVVSATLGGLRAETRE
                     NNGICSLTAQTMLRGTASRSRVQIARALEDVGGHLEVSSGRNSITVTATVLAQNLDTA
                     LDTVGDVLQHATFPEQELAAEKQQALEGLAQEQDDAFAASERLFLPYVYTRHPYGMEP
                     SGTPESVGGLTREAVVSLYDQLVRPERTVLALYGDFDATAARAAVEKAFAGWARGDHA
                     FVAPEPERPLAELRSATLTRPQGQTMVMFGFPGPRLADDDFYVREVLDAMLSGKGLPG
                     GPLHNTLRERGLVYMVHAWSADGLEPGHFTIVAATAPETADQAKAAIEEVVMNFLSTV
                     PSESDLAAGKQMCIAEHTLSLQQLSSRAQEAALNELYGLGFDRSAHYAERIQEVTADD
                     ILRVGRQLLDLKHCVTVVVGPERAAPAP"
     gene            2031..2219
                     /locus_tag="PLQ54_04515"
     CDS             2031..2219
                     /locus_tag="PLQ54_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92552.1"
                     /translation="MKKPSRSPLSEIQLLMLEEILADLDAYMFQQRSIHRLDPETGAA
                     EDEMDLGDLEPPSMREAG"
     gene            2357..3349
                     /locus_tag="PLQ54_04520"
     CDS             2357..3349
                     /locus_tag="PLQ54_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS92553.1"
                     /translation="MTSRERVIRTLRFEGPDRIPVDLWSLPAAWDRHGEGLRRLLERH
                     PTDFGGSGYEDPFYDPRAYQVGEYEDVWGCHWLQLRPGMIGEVKRAPLEDYAALESYR
                     PPVHLLDRGFERVRETLAADHTRFVNGWGGNPFERMQFLRGPENLYMDIASPSDQFVA
                     LRDMVFGFFEALVDRWVETDVDGITFLDDWGSQRGLLIRPEAWRSLFRPSYERLIGRA
                     KAAGKFVFFHSDGHIFDIYGDLIDMGVDAINSQLWCMDVGAIAARYAGKVTFWGEVSR
                     QDTLPRGTPDDVRAAARRMRELFFANGGLIGEGEVGGDVPLANVEALLTAWNEP"
     gene            complement(3346..3531)
                     /locus_tag="PLQ54_04525"
     CDS             complement(3346..3531)
                     /locus_tag="PLQ54_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92554.1"
                     /translation="MKNWSRIALAGCLALLVVAVPLAAGCKKGGDKDSDAKVLDLNEK
                     RTGGGLQGSTPAPGATR"
     gene            complement(3652..>4262)
                     /locus_tag="PLQ54_04530"
     CDS             complement(3652..>4262)
                     /locus_tag="PLQ54_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92555.1"
                     /translation="MADSGCVELRFGIESGSDEVLRRTKKGFRAVDVLPVLGKAVRVF
                     PRTDAFYMWGFPFETLDDFHQTVFQMVSARMLGARVLPSLLCFLPQTDIYAELENPEA
                     LEFCDELLPEYMLTGHEECRGSRVRIADSHRPIFDFIGQHKDIFPGFFHYDLAGNVLP
                     KLAVLQEFGFYIRDDVSALATSADTESCGAHSPHMGTAQLRA"
BASE COUNT          706 a         1347 c         1502 g          707 t
ORIGIN      
        1 gggacgtgac gaccgctgac gtgatcgatc gaacccggga gctgctgggc tcgctgccac
       61 gccgggcgct caaggccgtg gctcgggaga gcgagccgcc gcaaacggct ccccgacgcc
      121 gcaccatagt ggaccccgat ctcgacacgg cgtacctcat tatggcgtac cgcagcatcg
      181 acctactcca tccggatctc tacgcccttg atgtgctgtc gtatgtgctc tcgcagggcg
      241 cgagttcgcg gctggtgcgg accctgcggg atgagcagtc gctggtgtcg cacatcgaga
      301 cccactcgta cacgccgccg cgccggcccg gcgtgttcgc cgtcgaagcg atggtagacc
      361 cggcgaacct ggtgcccgcc gaggcggcca tcgcagacga gttgctccgc tgccagactg
      421 aggaggtaac gccagaggag ctggaccggg ccaaagctca gaaatcggcc gagctcgtgt
      481 ttgcccgcga gaccgtcgaa ggcctcgcgt cgcagctcgc gttcgactac tacgccacgg
      541 gcgatgtgcg tttcagcgag aggtacgtcg agggcatccg ccgggttacg gccgaagatg
      601 tgaagcgggt ggccgcgacg tatctgcgcc cggaggcgct ctgcgtggcc ctgctcacgc
      661 ccactgagtc cgaagcgtcc caggaggccg tcggggcgcc gtcggccgcc ccggttcaga
      721 aggtaacact cgacaacggc ctgaccctgc tcttgggcga ggaccacgcg gtgcccatgg
      781 tctcagtggt cagtgctacg ctgggcggcc ttcgcgccga gaccagggag aacaatggca
      841 tctgcagtct gacggcccag accatgctgc ggggcacggc gtcgcgtagt cgggtgcaga
      901 tcgcccgggc gctcgaggac gtgggcggcc atctcgaggt ctcctctgga cgcaacagca
      961 tcaccgtgac ggccacggtg ttggcgcaga acctggacac ggcgctcgac acggtgggcg
     1021 acgtgctgca gcacgccaca ttccccgagc aggagctcgc cgccgagaag cagcaggcac
     1081 tcgagggctt ggcccaggag caggatgatg ccttcgccgc gtctgagcgc ctgttcctgc
     1141 cctacgtcta cacgcggcac ccgtacggca tggagccgtc cggcacaccc gagtccgtgg
     1201 ggggactgac tcgcgaagca gtggtcagct tgtatgacca gttggtaagg cccgagcgaa
     1261 cggttctcgc tctctatggg gacttcgatg cgaccgccgc ccgcgcggcc gtggagaagg
     1321 cgttcgcagg ctgggctcgg ggcgaccatg cctttgttgc ccccgagccg gagcggccgc
     1381 ttgcggagtt gcggtcggcg acgctgactc ggccccaggg ccagacgatg gtgatgttcg
     1441 ggttcccggg ccctcggctg gccgacgacg acttctatgt gcgcgaggtg ctggacgcca
     1501 tgctctcggg gaaggggctg ccgggggggc cgctgcacaa cacgctgcgg gagcgggggc
     1561 tggtgtacat ggtgcacgcg tggtcggcag acggtctgga gccggggcac ttcaccatcg
     1621 tcgctgccac ggcgccggag accgccgacc aggccaaagc ggcgatcgag gaagtcgtga
     1681 tgaacttcct ttctacggta ccttcggaat cagatttggc tgcaggaaag cagatgtgca
     1741 tcgccgaaca tactctgtcc ttacagcagc tctcgagccg tgcgcaggag gcggctctga
     1801 atgagctcta cggcctgggc ttcgatcggt cagcccacta cgccgagcgg atccaggagg
     1861 tcactgcgga cgacatcctg cgggtgggca ggcagttgct ggacctgaag cactgtgtga
     1921 ctgtggtggt gggaccggag cgggcggcac ccgcgccgta gcagtaggga taggcgcggc
     1981 ggtgaacagc ctgaaggaga gcgtcgctta aggcgcggag ctgaggtcgg gtgaagaagc
     2041 ccagcaggag cccgttgtcc gagatccagc ttctcatgct tgaggagatc ctggccgatc
     2101 tggacgcgta catgttccag cagcgcagta tccatcgcct cgacccggag acgggtgccg
     2161 cagaggacga gatggatctg ggtgacctcg aacccccatc gatgcgcgag gcggggtagc
     2221 cggcagggac tgagcacttt gtctcgcgtg tcgtgtccga ccgagggggc agagccgttg
     2281 gccacccagg ccgtgccgga ggccagacgg gtgcgggcga acggctgagt gcccgggcga
     2341 gagggagtcc cctgccatga ccagtcgtga gcgcgtcatt cgcaccctcc gcttcgaggg
     2401 ccctgatcgg atcccggtcg atctgtggtc gttaccggcc gcttgggacc ggcacggcga
     2461 gggcttgcgc cgacttcttg aacgacaccc caccgatttc ggcgggtcgg gctatgagga
     2521 tccgttctac gacccccgcg cctatcaggt gggcgagtac gaagacgtat ggggctgcca
     2581 ctggcttcag ctccggccgg gtatgatcgg cgaggtgaag cgggcgccgc ttgaggacta
     2641 tgccgcgctc gagtcgtacc ggccgcccgt gcacttgctg gacaggggat tcgagcgcgt
     2701 gcgagagacg ctggccgcag accacacccg gttcgtcaac ggctggggcg gcaacccctt
     2761 cgagcgcatg cagttcctcc gcgggcccga gaacctctac atggacatcg ccagcccctc
     2821 ggaccagttc gtggcgctgc gcgacatggt cttcggcttc ttcgaggcgc tggtcgaccg
     2881 gtgggtcgag accgatgtcg acggcatcac gttcctggac gactggggta gtcagagggg
     2941 gttgctgatc cggcccgagg cctggcgcag cctgttccgg cccagttacg agcggctcat
     3001 cggccgtgcc aaggcggcgg gtaagttcgt cttcttccac agcgacggtc acattttcga
     3061 catctacggc gacctcatcg acatgggcgt cgatgccatc aattcgcagc tttggtgcat
     3121 ggacgtcggg gccatcgcgg cccgctacgc ggggaaggtc accttctggg gagaagtgag
     3181 ccgccaagac acgctgccgc ggggcacgcc agacgacgtg cgcgcggcag cgcggaggat
     3241 gcgcgagctg ttcttcgcca acggcggcct catcggcgag ggcgaggtag gcggcgatgt
     3301 gccactcgcc aacgtcgagg ccctgctgac ggcgtggaat gagccctagc gcgtcgcgcc
     3361 gggggcgggg gtgctgccct gcaggccgcc acccgtgcgc ttctcgttca ggtctagcac
     3421 cttggcgtcg gagtccttat ccccaccctt cttgcatccg gcggccagtg ggacggccac
     3481 gactagcagg gcgagacatc cggccaatgc gatacgggac cagttcttca ttcgtgccac
     3541 ttcctgtcgt ctgagttcgc ggaaccggca gcccttctgc ggcgccgggc acgaaggttc
     3601 cagttgggta actggttcgg gcgggcctcg cacgactcct tgccgccaac actacgccct
     3661 gagctgtgcg gtccccatgt gcggcgagtg ggcgccgcac gactcggtgt cggcgctggt
     3721 cgccagagcc gatacgtcgt cgcggatgta gaacccgaac tcctggagca ccgcgagctt
     3781 gggcagtacg ttccccgcca ggtcgtagtg gaagaagccg gggaagatgt ccttgtgctg
     3841 gccgatgaag tcgaagatgg gccggtgcga gtcggcaatg cgcacccggc tgccgcggca
     3901 ctcctcgtgc ccggtgagca tgtactcggg gaggagctcg tcgcagaact cgagcgcctc
     3961 cgggttctcc agctcggcgt agatgtcggt ctgcggcagg aagcacagca gcgaggggag
     4021 cactcgagcg cccaacatgc gagcggagac catctggaag acagtctggt ggaagtcgtc
     4081 gagagtctcg aaggggaacc cccacatgta gaaggcatcg gttcgcggga acacgcggac
     4141 agctttcccg agcacgggga gcacgtcgac ggcgcggaag cccttcttcg tccgacggag
     4201 cacttcgtcc gagcccgact cgatgccgaa ccgcagctcg acgcagccgg agtcggccat
     4261 ca
//
LOCUS       DAOSPL010000176         4262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20782_length_4262_cov_2.978369, whole genome shotgun sequence.
ACCESSION   DAOSPL010000176 DAOSPL010000000
VERSION     DAOSPL010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20782_length_4262_cov_2.978369"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2753
                     /locus_tag="PLQ54_04535"
     CDS             <1..2753
                     /locus_tag="PLQ54_04535"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92556.1"
                     /translation="MVSAALALTIAFGAAVAVDEPFNYFRNPWTLVGLQDYPDGTRIS
                     PRGSLVFASGAEVSLLAGAPPRVLGAENLWTLTRGRLPIAKLALKRDGVRYAIEVFAA
                     PMPQGGEWRYSGGDTNYVTHVWVRAANPGREAADSGFAIRWNEVGAPTRCDIRGDRAA
                     LTNERGDVAYAMPARSLETSVDRRAASWRLRLAPGQAVDLAIALPAQPVSPDAGVVEA
                     SPLAWREQCLRYWERTLARGSRFSFPEAKAEETFYASLVYQLIGNDEGVVKAGEGFYD
                     AFFLRDGSYQIWSYHLAGFGAEAHRAIEHLRTYQKPDGQFVSQEPELDGNGQALWAIW
                     NHYAFTRDRAWLAAAYPQMRRGVEWLRANRVTQASGDLADGVLPKAAADGENLWAGNN
                     QIVGYDVWNLRGVQATAWAAEALGRREEAAEYEALFEEYRQAIIDAVRRTGLGRFPPS
                     YQPGGTSWDNLSWLHPTVLIDPQDPRVGYTFDHERYGRYGFVEGTIRWSPWAGPLIHP
                     YMSTFLTNSALVRGEQERAVTDFYWYLLHTTSAHAFCEGLDYVTKLGWGDVIPHLWAA
                     ADYIILARNMLIREQGDDLHLLSAIPPSWLEPGKRTAAERAPTQFGEMSMAVSAEAVG
                     LWITLDPPRRNPPGRIILHLPPQFAVRFVTADGRSIVRQTDGSYVLNPATRNVRVTGR
                     LGAGMPLRTFASTVAEYRGREQTSAVLPPLVRLPLAEAVPESAWQSLDLRAQATTDPF
                     RAPFGVTVAPGPGAYLFTGLQTGQVVLEGVPFEVIDPSTNDGRGLVVLNGASACSGLP
                     RVAEIPVNQPARRVFLLGAVGGWAPADSGSDGHGLVARIVIDFTDGSARTIPLINGRT
                     IDDWASAPTATDVSTVLHGPERWHLNVLGVETGGKQVRRLRFIDEGTPTSPVLAAVTV
                     QR"
     gene            complement(2772..3131)
                     /locus_tag="PLQ54_04540"
     CDS             complement(2772..3131)
                     /locus_tag="PLQ54_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOS92557.1"
                     /translation="MSPDQPPSCIFCKIASGELESDIVYSDDQCVAFRDLNPQAPVHV
                     LLVPRKHIENLNGLTPDDRGLVGALVSAAPMVAAQLGLAVDGYRVVANVGRFGGQSVD
                     HLHLHILGGRPMRWPPG"
     gene            complement(3164..4177)
                     /locus_tag="PLQ54_04545"
     CDS             complement(3164..4177)
                     /locus_tag="PLQ54_04545"
                     /inference="COORDINATES: protein motif:HMM:NF015825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HOS92558.1"
                     /translation="MPVTNRGLLACLAVLGGAWAGPARAQSGDVYAWLERALQAGDLL
                     TYRAQCVAENHDPFTQSAARALKAQFVECCDGPSGRTRTEYMAPPELVNTMVLEDREF
                     IYQVDKKRRIALKQSAAAGSMLPSVEGSRIDLLRRNYSATIAGTRVIAGRECAIVEIM
                     PNADRAGNPWRRLFIDKRTAFPLRIENANGAGLLVNTKAVTSVDYAFRPPEGFFSLPP
                     GTTVKSDPVHRSGALQADASLSAQLGFRPVMPQYVPAGYVFAGAHIIGADGAKAVHLM
                     YFDGLSTISLFEEPTRSRGRAGWAPGGPGAHALSWTSGNLSLTLIGDILPQELVQMRN
                     SMG"
BASE COUNT          682 a         1401 c         1494 g          685 t
ORIGIN      
        1 tcatggtcag tgcggcactc gcgctcacga tagccttcgg cgccgctgtt gcggtggacg
       61 agccgttcaa ctacttccga aacccgtgga cactggtagg gctgcaagac taccccgatg
      121 gcacgcgcat ctcgccgcgc ggttcgctgg tgtttgcctc cggcgccgag gtgtcattgc
      181 tggccggagc gccgccgcga gtgctgggcg cggagaacct gtggacgctg actcgtggcc
      241 gcctgccgat cgcgaagctc gcgctcaaac gcgacggcgt gcggtacgcc atcgaggtgt
      301 tcgcggcgcc gatgccgcaa ggcggcgagt ggcgctactc gggcggggac acgaactatg
      361 tcacgcatgt gtgggtgcgg gccgcgaacc cgggccggga ggcggccgac tcgggattcg
      421 ccatccggtg gaatgaagtg ggcgcgccga cccgctgcga catccgcggg gaccgtgctg
      481 cattgacgaa tgagcggggc gatgtggcct atgcgatgcc cgcgcgaagt ctcgagacga
      541 gcgtggaccg acgagcggcg tcgtggaggc tgcgactggc ccccgggcaa gccgtcgatc
      601 tggccatcgc gctccctgcg caacccgtga gccccgatgc cggggtcgtc gaagccagcc
      661 cgttggcctg gcgcgagcaa tgcctgcggt actgggagcg gaccctcgcc cggggctcgc
      721 ggttctcgtt tcccgaggcc aaggccgagg agaccttcta cgcttcgctg gtgtaccagc
      781 tcatcggcaa cgacgagggt gtcgtcaagg cgggcgaggg gttctacgac gccttcttcc
      841 tgcgtgacgg gtcctaccag atctggtcat accacctggc gggatttggg gccgaagccc
      901 atcgagccat agaacatctg cggacctacc agaagcccga tgggcagttc gtgagtcagg
      961 agcccgaact ggacggcaat ggccaggctc tatgggcgat ctggaaccac tatgccttca
     1021 cacgagaccg cgcatggctg gcggccgcgt atccgcagat gcgtcgaggc gtcgaatggc
     1081 tccgggccaa ccgggtgacg caggcctcgg gcgacctggc ggacggcgtg ctgcccaagg
     1141 ccgccgccga tggcgagaac ctttgggccg gcaacaacca gatcgtcggc tacgatgtgt
     1201 ggaacctccg cggtgttcag gccacggcct gggcggccga agcactcggc cgcagggagg
     1261 aggccgccga gtacgaggcg ctgttcgagg agtaccgtca ggcgatcatc gacgccgtcc
     1321 gccgcactgg tttggggcgc ttcccgccca gctaccagcc gggcgggacc tcatgggaca
     1381 acctctcgtg gctgcaccca acggtgttga tcgaccccca agacccgcgg gtcgggtaca
     1441 ccttcgacca cgagcgctac ggccgctacg gcttcgtcga ggggaccatc cgctggtcgc
     1501 cctgggccgg gcctctcatc cacccctaca tgtcgacctt cctcacgaac agcgcgctgg
     1561 tgcgcggcga gcaggaacgg gcggtcactg acttctactg gtacttgctg cacaccacat
     1621 cggcccacgc gttctgtgaa gggctggact atgtaaccaa gctggggtgg ggtgacgtca
     1681 tcccgcatct ctgggcggcc gccgactaca tcatcctcgc tcggaacatg ctcatccgcg
     1741 agcaggggga cgacctgcac ctgctgtcgg cgatcccgcc gtcatggctc gagcccggca
     1801 agcgaacagc tgccgagcgc gcgcccacgc agttcgggga gatgagcatg gcggtgtcgg
     1861 ccgaagcggt ggggttgtgg atcacgctgg atccgccgcg gcgcaatccg ccgggtcgca
     1921 tcattctgca cctgcctccg cagttcgcgg tacggttcgt gaccgccgat ggccggagca
     1981 tcgtccgcca gaccgatggc tcctatgtac tcaacccggc cacgcgcaac gtgcgggtca
     2041 ccggccgtct cggcgccggc atgccgctgc ggaccttcgc ctccaccgtg gccgagtacc
     2101 gcggcagaga acagacgagc gcggtcttgc ctccactggt gcggcttccg ctggcggagg
     2161 ccgtgcccga gtcggcctgg cagtcgctcg acctgcgcgc ccaagccacc acggacccgt
     2221 tccgcgctcc gtttggcgtg acggtggcgc cggggccggg cgcgtatctg ttcaccggct
     2281 tgcagacagg gcaggtggtg ctcgagggcg tgccctttga ggtgatcgat ccgtcgacca
     2341 acgatgggcg ggggctggtg gtgctgaacg gcgcgtcggc ctgttccggc ctgccgcggg
     2401 tcgccgagat acctgtcaat cagccggccc ggcgcgtgtt cctcctcggc gccgtgggcg
     2461 gatgggcccc ggccgactcg gggtccgacg ggcacggcct ggtggcccgg atcgtgatcg
     2521 acttcaccga cggtagcgcc aggacgatcc cactgatcaa cgggaggacc atcgacgact
     2581 gggcctcggc gccgacggcg acggacgtat cgacggtgct gcacggcccc gagaggtggc
     2641 acctgaacgt gctcggagtc gagacgggcg gcaagcaggt ccgccgcctc cggttcatcg
     2701 atgagggcac cccgaccagc cccgtgctgg ccgcggtgac cgtgcagaga taggcggccc
     2761 ggtcgggcgc gctatcccgg cggccagcgc atggggcgtc cgcccaggat gtgcaggtgc
     2821 aggtgatcga ccgattgccc gccgaaacgg cccacgttgg cgacaacccg gtagccgtcg
     2881 acggcaagcc ccaactgagc ggcgaccatc ggcgccgcgg agaccagtgc ccccaccagg
     2941 cccctgtcgt ccggcgtcag gccgttcagg ttctcgatgt gtttccgcgg gacaagcagc
     3001 acgtgtaccg gagcctgcgg gttgaggtcg cggaaggcga cacactggtc gtcggagtag
     3061 acgatgtcgc tctcgagctc gcctgaggcg atcttgcaga agatgcagct gggcggttgg
     3121 tctgggctca cgggcgcaac tccttcgagc gcggggccac aatctacccc atcgagttcc
     3181 gcatctgaac gagctcctga gggagaatat ccccgatcag cgtaaggctc aggttgccgg
     3241 atgtccagga cagcgcatgg gcccccggcc cccccggggc ccagcccgcg cggccgcgag
     3301 agcgtgtggg ctcctcgaag agcgagatgg tgctgaggcc atcgaagtac atgagatgaa
     3361 cggccttggc gccgtccgcg ccaatgatgt gtgccccagc gaacacatag cccgcgggga
     3421 cgtactgggg catgacgggc cggaacccca gctgagcgct gagtgacgca tcggcctgaa
     3481 gggcgccact gcgatgcacg ggatcggact tcaccgtggt acccgggggg aggctgaaga
     3541 acccctccgg gggccggaag gcgtagtcca cactggtgac ggcctttgta ttgaccaaaa
     3601 gccccgcgcc attggcgttc tcgatgcgta gcgggaaggc cgtgcgcttg tcgatgaaca
     3661 agcggcgcca tgggttgccg gcgcgatcgg cgttgggcat gatctcgaca atggcgcact
     3721 cgcggcccgc gatgacccgc gtgcccgcga tggttgccga gtagttccgt cgcaggagat
     3781 cgatacggga accttcgacc gatggcaaca tactgcccgc ggccgccgac tgcttgaggg
     3841 cgatccggcg cttcttgtcg acttggtaga tgaactcacg gtcttcgagc accatggtgt
     3901 tcaccaactc gggcggcgcc atgtactcgg tgcgcgtccg gcccgacggc ccatcgcagc
     3961 actcgacgaa ctgggctttg agggcccggg cggccgattg ggtgaatggg tcatggttct
     4021 cggcgacgca ctgggcgcga taggtcagta gatcccccgc ctgcaacgcc cgctcgagcc
     4081 aggcgtagac atcgcccgac tgggcacggg ctgggccggc ccaggcaccg cccaggacgg
     4141 ccagacaggc tagaagaccg cggttcgtga ctggcatcgt gcggtggatc cccctgttga
     4201 tggtcagctt tgcccgccag gacctactcc agtagggaca ggctttcgag gttcacggcc
     4261 ac
//
LOCUS       DAOSPL010000177         4248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20872_length_4248_cov_2.487241, whole genome shotgun sequence.
ACCESSION   DAOSPL010000177 DAOSPL010000000
VERSION     DAOSPL010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20872_length_4248_cov_2.487241"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLQ54_04550"
     CDS             complement(<1..222)
                     /locus_tag="PLQ54_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOS92559.1"
                     /translation="MNSHTAPERTPLRRTAVLQAAVELADRVGLAEVSMRTLARTLGV
                     VPMALYKHVANKDELLGGMVDAVIAEIPPA"
     gene            339..1055
                     /locus_tag="PLQ54_04555"
     CDS             339..1055
                     /locus_tag="PLQ54_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HOS92560.1"
                     /translation="MTPTRRTALIAGLFYLVTFVSIPTLTLYADAKTRGFTTGLGGDS
                     GAVLGGFLEVVVGLAGIGTAVTLYPVVRRHGEQLALGFVAARVLEAAMIFTGVTAIMS
                     LVSLHRSGSGATDSAALVVMGDSLVSTYNWAFLLGQSLMPALNALLLGTLLVRSGLVA
                     RAIPLIGLVGAPIHLTAVVLSFFGVIAQVSTWTLLAALPIAAWEFSLGVYLLVKGFRR
                     IPMAPAAESLRVAQSEVPAR"
     gene            complement(1031..1429)
                     /locus_tag="PLQ54_04560"
     CDS             complement(1031..1429)
                     /locus_tag="PLQ54_04560"
                     /inference="COORDINATES: protein motif:HMM:NF023542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HOS92561.1"
                     /translation="MPRSVLGPAPGPFFFGSKADLAIGAVISSDQVTDPTFGGGTAYL
                     HFTTALDLATWRAQLLPGEEPGRVYRVVPTGPFAAVAIGSRSGHAPWTAYPWYRTTEP
                     VRVTAEILEWEPHPESRIEGLRDHLAGTSL"
     gene            complement(1474..1842)
                     /locus_tag="PLQ54_04565"
     CDS             complement(1474..1842)
                     /locus_tag="PLQ54_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HOS92562.1"
                     /translation="MPGSSPVRVARVYDPPDPTAGQRVLVDRLWPRGLSKQRAALTLW
                     CKEVAPSTELRRWYAHDPAKFTEFATRYRSELRDDTHAAALAGIRQLMTTGPIVLLTA
                     SRRSDLSEAAVLESILSGTP"
     gene            complement(2039..2365)
                     /locus_tag="PLQ54_04570"
                     /pseudo
     CDS             complement(2039..2365)
                     /locus_tag="PLQ54_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007300330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            complement(2390..2884)
                     /locus_tag="PLQ54_04575"
     CDS             complement(2390..2884)
                     /locus_tag="PLQ54_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92563.1"
                     /translation="MSQQSVPEPSFDTPLSTAAERAASGAADRALEHIYVLLKTLFTV
                     APIVFGLDKFANVLTQWDGYLAPWINNIVPGTATQAMYAVGVIEIVAGVLVAIRPRIG
                     SLVVAAWLGGIIVNLLTYSGFYDVALRDAGLLVAALCLYVAATAHDAVAGAGRARANA
                     RGTW"
     gene            3047..3523
                     /locus_tag="PLQ54_04580"
     CDS             3047..3523
                     /locus_tag="PLQ54_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92564.1"
                     /translation="MLDVLRSWSRRRWIAAGGAAVLTVLVMAVPTALIPTPVFGREIP
                     PTPWAWPVLLVSAALSGLLFATYVRERGSVDPPPEGAGVDGRGTAGALLTFFAVGCPV
                     CNKLVLLALGYAGALQWFAPVQPYLAGLAIVLLMWALVVRLRGQVACPVTSSEVGG"
     gene            3525..4199
                     /locus_tag="PLQ54_04585"
                     /pseudo
     CDS             3525..4199
                     /locus_tag="PLQ54_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005516217.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
BASE COUNT          589 a         1452 c         1472 g          735 t
ORIGIN      
        1 tgcaggcggg atctcggcga tgacggcgtc gaccatgccg ccgaggagtt cgtccttgtt
       61 ggcgacgtgt ttgtagagcg ccatcggcac gaccccgagg gtccgggcga gggtgcgcat
      121 gctcacctcg gcgaggccga cgcgatcagc caactcgacg gccgcctgca acacggccgt
      181 ccggcgcagc ggggtccgct cgggtgcggt gtgactattc acggcgctca ctcttccgga
      241 tccacttgac gggtgtacag cgtacacgta ccgtcatctg cacctgaggt gtacgccgta
      301 cactctccat ccctgcagat cccgtctgga gctcgcccat gacccccacc cgccgcacgg
      361 ccctcatagc cggcctgttc tacctcgtca cgttcgtgtc gatccccacc ctcacgctct
      421 acgccgacgc caagacccgc gggttcacca ctggcctcgg cggcgactcg ggagcagtcc
      481 tcggcggctt tctcgaggtg gtggttgggc tggccggtat cggcaccgcc gtgaccctct
      541 accccgtggt ccggcgacat ggcgagcagc tcgccctcgg cttcgtcgcc gcgcgcgtgt
      601 tggaagcagc gatgatcttc accggggtca ccgccatcat gtcgctcgtc tccctgcacc
      661 gatccggctc tggcgcaacg gattccgcgg ccctggtggt catgggtgac tcccttgtct
      721 cgacctacaa ctgggccttc ctgctgggtc agagcctcat gccggcgctc aacgctctgc
      781 tgctcgggac cctgctcgtc cgctccggtc tggttgcccg agccatcccg ctcatcggcc
      841 tcgtcggcgc ccccatccac ctcacagccg tcgtgttgag cttcttcggc gtcatcgcgc
      901 aggtctctac ctggactctg ctcgcggccc tgcccatcgc cgcctgggag ttctccctcg
      961 gtgtctacct cctcgtcaaa ggcttccgcc gcatcccgat ggcacctgct gccgaatcgc
     1021 tgcgagtggc tcagagcgag gtgccggcca ggtgatcgcg tagtccctcg atccgcgact
     1081 ccggatgcgg ctcccactcc aggatctcgg cggtcacccg caccggctcg gtggtccggt
     1141 accacgggta tgccgtccac ggcgcgtgtc cgctcctcga cccgatggcc acggccgcga
     1201 acggcccggt cggcacgacc cgatagacgc ggccgggttc ctcgcccgga agcagctgcg
     1261 cccgccaggt cgcaaggtcc aaagccgtcg tgaagtgcag gtatgccgtg ccgccgccga
     1321 acgtggggtc ggtcacctgg tcggagctga tgacggcgcc gatggccagg tcggccttgc
     1381 tgccgaagaa gaacgggccg ggcgccggac ccagcactga tctgggcact gatctgggcg
     1441 cggagctgga cgcggagtgt gaggccgtcg atctcatggc gtgccgctca ggatcgactc
     1501 gagaacggcc gcctcgctga ggtcgctacg acgcgatgcg gtgagcagga cgatcggtcc
     1561 ggtggtcatg agttgccgga tccccgcgag cgcggccgcg tgagtgtcgt cgcggagttc
     1621 ggatcgatat cgcgtcgcga actcggtgaa cttcgcgggg tcgtgggcat accagcggcg
     1681 caactccgtg gacggggcca cctccttgca ccacagcgtc agggcggcgc gttgcttgga
     1741 aagtccgcgt ggccacagcc ggtcgacgag cacgcgctga ccggctgtgg gatcgggcgg
     1801 gtcatagacc cgggccaccc gcaccggtga gctgcccggc ataccgtcag cccccggtgc
     1861 gtggaggata gaccgggccg ccctcccaga tctcgcggga gatcgcgcgc tgctcgttga
     1921 acccagcgcc atcggaggtg tcacgacggg catccccgcc cccaggggtg ccatccgcag
     1981 gggcgtcatc cgcaggagtg tccgcacgag ggcgatcctc acgggctgct gtgggggctg
     2041 cgcggtccag ggccgtgatc gcgagcacgg agtgggcgta ggcgcccccg gcgcgcatct
     2101 cggccgcgac ccagatcgcc tccatcacct gctcgtccgt ggcgccggcc cggtgagcga
     2161 gtgtggtgtg accggtgatg cagtaggggc actgcgtcgt gtgcgcgacg gcgaccgcaa
     2221 tgagttgctt ggtgcgttcg tcgagggcgc cgtcagcaaa gaccgccgcg ctgaaggcct
     2281 cgaaggccgc gtggatatgc ggcgcgaggg ccacccgccg gccgtgcaac tgcggggtga
     2341 ctcggggata ggggctgtcg gacatctcga ttctcctgtc gcgtcgggat caccaggtgc
     2401 cccgggcatt ggcgcgggcg cgcccagcac cggcaacggc atcgtgggcg gtggccgcga
     2461 cgtagaggca cagggccgcg accaacaagc ccgcatcgcg cagcgcgacg tcgtagaagc
     2521 cggagtaggt gagcaggttg acgatgattc cgcccagcca cgcggcgacg accaacgagc
     2581 cgatgcgcgg ccggatggcc acgaggacac cagcgacgat ctcgatgacg ccgacggcat
     2641 acatcgcttg agtagcagtc ccgggcacga tgttgttgat ccacggggcg agatagccgt
     2701 cccattgggt gagcacattg gcgaacttgt ccagcccgaa gacgatcggc gccacggtga
     2761 agagcgtctt gagcaggacg tagatgtgct ccagcgcccg gtcggcggct ccggacgcgg
     2821 cgcgctcggc ggcggtcgag aggggggtgt cgaaggacgg ttccgggacg gactgttggg
     2881 acatgggtgt cgcctcctgc tggtcacagg ggttgcccgg aaccgcccca gctcaaaccc
     2941 gtcagacgtg cggcccctcg cccagcacgg cggggggcga cccggcggag gccgacacgg
     3001 cataccgggt gctcttcccc tcgcgccgga aacgtaagat cggacgatgc tcgacgtgct
     3061 gcgctcgtgg tcgcggcggc ggtggatcgc ggcggggggc gcggccgtgc tgacggtcct
     3121 ggtcatggcg gtgccgaccg ccctcatccc caccccggtg ttcggccgag aaatcccgcc
     3181 cacgccgtgg gcctggccgg tgctgctcgt gagcgcggcg ctgagcgggc tgctcttcgc
     3241 cacctatgtg cgtgagcggg ggtccgtcga tccaccaccg gagggcgccg gagtggacgg
     3301 tcgcggcaca gcgggcgcgc tgctcacctt ctttgcggtc gggtgcccgg tgtgcaacaa
     3361 actcgtgctg ctggcgctgg gctatgccgg tgcgctccag tggtttgccc cggtgcagcc
     3421 ctatctggct ggcctggcca tcgtcctgct gatgtgggcg ctggtggtgc ggttgcgcgg
     3481 tcaagtcgcc tgtccggtca cgtcttccga ggtcggaggc tgaggtggcg gtctggctgt
     3541 ccttgctcct cggagtggtg gtcgtcgtgg cgatcaccgc gctgaccgcc tacttcgtcg
     3601 cgcaggaatt cgcctacctc gccgtcgatc gaccggccct cgcagccgcg gccgctcgtg
     3661 gtgatgcgcg cgccgagcgg gcattggcga tcacccggcg cacgtcattc atgctgtcgg
     3721 gggcgcagct cggcatcacc gtgaccggtc tgctcgtggg ctatgtcgcc gagccgctca
     3781 tcggcgagtc cctcgcggcg ttgctcggtg ggtcatccac cgggtgggcg ctggccatcg
     3841 gcgcgaccgt tgccctggtc ttctcgacac tggtgcagat gcccttcggc gagttgctgc
     3901 ccaagaacta cgcgatcgcc cgggccgatg gctcggcacg cgccctggct cgctcgactc
     3961 gtctctatct ggcggtcttc gggccgctca tctgggtctt cgaccgggcc tccgagctgt
     4021 ttttgcgggt gctcgggatc caggccgttc atgacgtcga ccacagcgcg acccgagacg
     4081 acctcgagca cgtcatcgcc cgctcccgtg aggagggcca actcactgcg gagctgtcca
     4141 cggtgctgga tcgcatcgtc gacttcccac gccaagacgt cgagcacgcc atgattccgg
     4201 attatggcac caccagcatg atagcgggca acccttcatg aaaaaggt
//
LOCUS       DAOSPL010000178         4235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20972_length_4235_cov_3.806938, whole genome shotgun sequence.
ACCESSION   DAOSPL010000178 DAOSPL010000000
VERSION     DAOSPL010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20972_length_4235_cov_3.806938"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PLQ54_04590"
     CDS             <1..728
                     /locus_tag="PLQ54_04590"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOS92565.1"
                     /translation="DRMLAASVLGIDLIVGGHSHSKLDRPVQVVHPGTPSCTWIVQAG
                     DLSRFVGRVDIEAEQHEAGWRITRVGGRLMPVDGSVEEAPDIRKFLEDHQERLAQVVG
                     RNPAELPNAREGQDTVGQFAAEAVLRATGAEVALLGRDAFDAGLPAGPVTLADVYRIH
                     PWRSPVLVAQLNAEQIRQAREELDLLVAGAEVTPGQTYAVAVDGFTLGGAPLLREAPF
                     TDSGLRLDEVLIGSIRARSPAGE"
     gene            855..1712
                     /locus_tag="PLQ54_04595"
     CDS             855..1712
                     /locus_tag="PLQ54_04595"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOS92566.1"
                     /translation="MRPGLVRVGLWHRWLCALAACLCVPYVSWRATAADGFGVDGAAA
                     VPPGSRVSPSNTRAPGEAGFTVVSDIDGMELVLVPGGEFLYGEDRVACELPSFYIDKH
                     EVTNDQFARFVAATGYAPRRWHYIEGRGSHPAVNVSLLDAEAYAAWAGRAIPTEGQWE
                     KAARGTDGRPYPWGAEWHDTYAITPECGVKGDVPVGSVPLGASPYGALDMVGNVWEWT
                     VSPYRGDSDGGRRALRGGAWDAHKGKRRANCTYRSKFPPERTDGKLGFRCVWPAQPWA
                     SPVAGGERG"
     gene            1736..3661
                     /gene="mrdA"
                     /locus_tag="PLQ54_04600"
     CDS             1736..3661
                     /gene="mrdA"
                     /locus_tag="PLQ54_04600"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOS92567.1"
                     /translation="MEGSGTGSDLLQRRALLLRVAAIAALTIIWARLWHMQVWQREQH
                     RETSESKLLVKASIAAPRGQILDREGRPLANNRATWAVAVTPDQVPEYRRRHTPESFR
                     RIAAELERIFGGVLTAEEIQRRASEGLPAESFRPRLIVDDVSEVTRCRIKELGPELGG
                     VDVVQRLVRAYPHPEGANAFHAVGYASNLKAEQIGLLKQGYEYSDVVGQLGIEDVYEA
                     ELRGIKGKEVIDARGQPSVEKAPVPGRDLTLAMDARIQAAAEKALGRWVNTSGRRGGA
                     AIVMDCRNGQVLALASYPAFAPEWCTSQATPEERRRFAELASQKASGLPWVNHAISGQ
                     RCPASTFKPITLATALEMGAIDPTTHVYCSGKMKVSEEQGRVRTKNCWKKSGHGWVGV
                     REAIAFSCNVFCYHAGRELNRLWRDDPRKREFQQIGARAFGFGRRSGIDLREESAGFV
                     GDKAWIFRQRQLQDDAPPDWYVGHSMDMAIGQNVIEGTPLQVACATAAIANGGTLVWP
                     RLVKEVRQDGRIVPYRHPLAEPVKRGPIASLSPETLQLVREGMRLAVAHREGTAHAAF
                     ESFTRTEVAGKTGTDEVWDNSWFTSFAPAGNPKVVVVVFIEKGGHGSESAAPAAREIY
                     EALFAPDGRLQVHLDSQ"
     gene            3802..>4235
                     /locus_tag="PLQ54_04605"
     CDS             3802..>4235
                     /locus_tag="PLQ54_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS92568.1"
                     /translation="MGQGTWLCQIPNVIRVFGRHRQLLRWWWGSRARRLSLARECWFG
                     LDGDVRPPRSVSLRPTFLCNLNCLMCAFANSAADGDMPITRASDMLALDVAKRLTDDL
                     APHHTCLTVTGGEPFLWPPMFELFDYARSRGVLTTVTTNGTLL"
BASE COUNT          691 a         1354 c         1514 g          676 t
ORIGIN      
        1 cggaccgcat gctcgcggca tccgtcttgg gcatcgatct gatcgtcggc ggccacagcc
       61 actcgaagct cgaccggccg gtgcaggtgg ttcatcccgg tacaccgagt tgcacatgga
      121 tcgtccaggc cggcgacttg tcgcgcttcg tgggccgggt ggacatcgag gccgagcaac
      181 acgaggcagg ctggcgcatc acccgcgtgg gcggccgcct gatgccggtc gacggcagcg
      241 tggaggaggc gccggatatc cgcaagttcc tcgaggacca tcaggagcgg ctggcccaag
      301 tggtcggccg gaacccggcc gagctgccca acgcgcggga gggccaggac accgtggggc
      361 agttcgcggc cgaggcggtg ctgagagcca cgggcgcgga ggtagcgctg ctgggccggg
      421 acgcgttcga cgccggtctg ccggcggggc ccgtgactct ggccgacgtg taccgcattc
      481 acccgtggcg cagtccggtg ttggtcgccc agctcaacgc cgagcagatt cgccaagcaa
      541 gggaggagct ggacctgctg gtggccggtg ccgaggtgac acccggacag acgtacgcgg
      601 tggcggtgga cggcttcacg ctgggcggtg cgccgctcct ccgcgaggcc cccttcacgg
      661 actcgggcct ccggttggat gaggtgctga ttggctccat ccgggctcga agcccggccg
      721 gtgagtgaac atgggtccgg ggttcagccg ctgatggatg ggccggcccg gccggtataa
      781 tgggcatcga ctggccgggg cacctcactg accgaggtgg ggccgactcc ggaggcgtcc
      841 gcgcgggagg gtgtatgcga ccggggcttg tgcgtgtggg tctgtggcac cgatggctgt
      901 gtgcactggc agcgtgtctg tgtgttccgt acgtgagctg gcgggctacc gcggcagatg
      961 ggttcggcgt cgatggagcc gctgcggtcc ccccgggctc tcgcgtgagc ccgagcaaca
     1021 cgcgggcgcc gggcgaagcg ggcttcaccg ttgtgtccga catcgacggc atggagctgg
     1081 tgctggtgcc tggtggggag ttcctctacg gtgaggatcg ggttgcctgt gagctaccga
     1141 gcttctacat cgacaagcat gaggtcacca acgatcagtt tgcccgattc gtggccgcaa
     1201 ccggctatgc cccgcggcgt tggcactaca tcgaggggcg cggaagccat ccagccgtga
     1261 acgtctcgct cctggacgcc gaggcctacg ccgcctgggc gggtcgggca attccaaccg
     1321 agggacagtg ggagaaggcg gctcggggca ccgacggccg gccctacccg tggggtgccg
     1381 agtggcatga cacctatgcc atcacgcccg aatgcggcgt caagggggac gtgccggtgg
     1441 gcagtgtccc cctgggcgcc agcccctatg gagcactgga catggtgggc aatgtctggg
     1501 agtggactgt gtctccctat cgaggcgatt ccgatggtgg gaggcgagcc ttgcgcggtg
     1561 gtgcatggga cgcgcacaag ggtaagcggc gcgccaactg cacgtaccgg agcaagttcc
     1621 cgccggaacg cacggacggc aaactcggct ttcggtgtgt ctggccggcg cagccgtggg
     1681 cgtccccggt cgcgggggga gagagaggct agcacccgcg gacagcgggt caccgatgga
     1741 gggctcgggc accggttctg atctgctgca gcggcgggca ctcctgctgc gcgtggcagc
     1801 cattgcggcg ctgacgatca tctgggcgcg cctgtggcac atgcaggtct ggcagagaga
     1861 gcagcatcgg gagacgagtg agagcaagct cctggtcaag gcatcgatcg cggcgcctcg
     1921 cggccagatc ctggaccgcg agggccgtcc tcttgccaac aatcgagcca catgggcggt
     1981 ggcggtgacc ccagaccagg tgcccgaata ccggcggagg cacacgccgg aatcattccg
     2041 tcgcatcgcc gcggagctcg agcgcatctt cggcggcgtg ctgaccgccg aggagatcca
     2101 gcgccgtgcc agtgagggac tgccagcgga atccttccgc ccccgcctca ttgtcgacga
     2161 cgtctccgaa gtgacgcgtt gccgcatcaa ggagctgggc ccggagctgg gaggcgtcga
     2221 tgtcgtccag cgcctggttc gtgcctatcc acaccccgag ggcgccaatg cattccacgc
     2281 cgtggggtat gcatccaatc tcaaagccga gcagatcgga ctcctcaagc agggctatga
     2341 gtacagcgat gtcgttggcc agctcgggat cgaggacgtg tacgaggccg aactgcgcgg
     2401 catcaagggc aaggaggtca ttgacgcccg cggccagcct tccgtcgaga aggcgccggt
     2461 gcctggccgc gacctgacgc tggccatgga tgcccggatc caggcggccg ccgagaaggc
     2521 gctgggccga tgggtgaaca ccagcggccg acggggcggg gctgccatcg tcatggactg
     2581 tcgcaacggg caggtgctgg ccctggcgag ctacccggcg ttcgcccccg agtggtgcac
     2641 gtcgcaagct acccccgagg aacggcggcg gttcgcggag ctggcgtcgc agaaggcctc
     2701 cggcctgccc tgggtcaacc atgccatcag cggccagaga tgcccggcgt cgacgttcaa
     2761 gccaatcacg ctggccacgg ccctggagat gggggcgatc gacccgacca cgcacgtgta
     2821 ctgctcggga aagatgaagg tgagtgagga gcagggccgg gtacggacca agaactgctg
     2881 gaagaagagc gggcacggct gggtcggggt ccgagaggcc atcgccttct cctgcaatgt
     2941 gttctgctac catgccggcc gagagctcaa ccggctgtgg cgagacgacc cccgcaaacg
     3001 cgagttccag cagatcggag ctcgcgcgtt cggcttcggc cgccgcagcg gcattgacct
     3061 gcgcgaggag tcagcgggct tcgtcggtga caaggcgtgg atcttccggc agcgccaact
     3121 ccaggatgac gcacctcccg attggtatgt gggccactcg atggacatgg cgatcggcca
     3181 gaacgtgatt gagggaacac ctcttcaggt ggcctgcgcc acggcggcga tcgccaatgg
     3241 aggcacactc gtctggccgc gactggtcaa agaggtgcgg caagacggcc gcatcgtgcc
     3301 atatcggcac ccactcgcgg agcccgtgaa acgtggcccc atcgcgtcac tctcccccga
     3361 gaccctgcag ctcgtgcgcg agggaatgcg cctggccgtg gcgcaccgcg agggtacggc
     3421 acatgccgcc ttcgagtcgt tcacccggac cgaggtggct gggaagaccg gcacggacga
     3481 ggtctgggac aacagctggt tcacatcctt cgcccctgca ggcaacccca aggtggtggt
     3541 cgtggtgttc atcgagaaag gcggccacgg ttccgagtcg gcggcgccgg cggcgaggga
     3601 gatctacgag gcgctcttcg ccccggatgg gcgcctccag gtacacctcg attcgcagta
     3661 gtccttgccc cgtcagcccg aggagggcaa gccctcgggt aggccgaaag gtcgcgtgtc
     3721 ttctgccacg cagaaccgag ctcgcacgtg gcacacaccg gcgcgtccct cgggcgacaa
     3781 tgcgatggag tgtagtgtcc cttgggtcag ggcacttggc tgtgccagat ccccaatgtg
     3841 atccgagtct tcggccggca ccgccagttg ctccgttggt ggtgggggag ccgcgcacga
     3901 aggctgtcac tcgctcgcga gtgctggttc ggcctggatg gtgacgtgcg gcccccacgc
     3961 tcggtcagcc tccgtcccac ctttctgtgc aacctcaact gcctcatgtg cgccttcgcc
     4021 aactccgccg ccgacggcga catgcccatc acacgggcca gtgacatgct ggccctcgat
     4081 gtggcgaagc gcctgaccga cgatctggca ccgcatcaca catgcctcac ggtcaccggg
     4141 ggcgagccat tcctctggcc gcccatgttc gagctgttcg actacgcccg atctcggggt
     4201 gttctcacca ccgtcacaac caacggcacg ctact
//
LOCUS       DAOSPL010000179         4232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_20992_length_4232_cov_3.634427, whole genome shotgun sequence.
ACCESSION   DAOSPL010000179 DAOSPL010000000
VERSION     DAOSPL010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20992_length_4232_cov_3.634427"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /gene="flgK"
                     /locus_tag="PLQ54_04610"
     CDS             complement(<1..707)
                     /gene="flgK"
                     /locus_tag="PLQ54_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOS92569.1"
                     /translation="MMTRSTFFGIELGKRAIMAQRAGTDVVGHNLANQSTEGYSRQSL
                     YLVSSSPFATPTTAPYNMAGQLGTGVMAETIARAQNEYITNQIRGSTSRQARLDVLAD
                     TYSQLEGIVDVSSDASLDQLLTDFFSAWHDLSLSPEETANRTALREAASTLASEFGRI
                     DAALTAIREQLDGDIQLMVDTVNRIGADLAAVNHEIQKVNASDQVPNDLMDRRDALLL
                     ELSSYVSITSLIQEDSSV"
     gene            complement(755..1258)
                     /locus_tag="PLQ54_04615"
     CDS             complement(755..1258)
                     /locus_tag="PLQ54_04615"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HOS92570.1"
                     /translation="MPPVADLFHQLERILEEQLAGLRELLSLMGAQEEALVTGQPDRI
                     RPLVRLQEAAASRLECAEERRQAASAKLAGALGLPPDAALREIARSLPASDAQRALAL
                     LGDMTRTMGDLASSSRRNEALVRHAVASTRHAIAVLTEAQLRADGRYVPPGRPRRATQ
                     GVVDCQV"
     gene            complement(1378..1683)
                     /gene="flgM"
                     /locus_tag="PLQ54_04620"
     CDS             complement(1378..1683)
                     /gene="flgM"
                     /locus_tag="PLQ54_04620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOS92571.1"
                     /translation="MLVTNRNVQDLLRSFAQRTSRTEDVARAEVAKPAESTVVRKSDE
                     VVLSQEAQEVHRALRALKETPEVREDLVADLRQRIQDGTYKVDSEEIAELLLSDDDE"
     gene            complement(2063..2425)
                     /locus_tag="PLQ54_04625"
     CDS             complement(2063..2425)
                     /locus_tag="PLQ54_04625"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HOS92572.1"
                     /translation="MALELLGAGDVPASALNVADLNSLRKLGETSAGLRAAAQEFESF
                     LLYQLVQSMRKTIHKVEMFHGGRAEEMWEGMLDQELTRELAKAGGIGLAKMIYDQMAS
                     QVESEDSAPPGALVAEQR"
     gene            complement(2458..3600)
                     /locus_tag="PLQ54_04630"
     CDS             complement(2458..3600)
                     /locus_tag="PLQ54_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOS92573.1"
                     /translation="MLRWRVCHVWTALLLVAATAPSLAQAPIGSGTTGPRTRLKDIGK
                     VQGVRSNQLFGLGIVVGLDGTGDSQQAVFTSQALLNLLAQLGVNVEGQVRTQNVATVM
                     VTAELPAFVKPGDRIDVTVSSIGNAKSLQGGQLIQTPLQGADGDVYAVAQGPITIGGF
                     NATGGGGGERVTKNHPTAGRIPNGAIVEKEVEMEMVQERSLTVTLSHADFSTASRAAA
                     AINEAIPRASARAIDASSIRVSLPPDAEFSLIDLIAQIETVELTVDTPAKIVINEKTG
                     TVVMGGNVRVQPVAVAHANLSVQVSATPVVSQPPPLSSGQTVAGTKTKLTVTEGQSRL
                     MPVMGGDTIDDVISSLSAIGATPRDMIAILQAMKSAGAILAEIEMQ"
     gene            complement(3613..4218)
                     /locus_tag="PLQ54_04635"
     CDS             complement(3613..4218)
                     /locus_tag="PLQ54_04635"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOS92574.1"
                     /translation="MRSHRAVPALLACAAALAVARGASARSLWVAPDGLSPKGSRTLI
                     ADPVARQVGDVVTILISETTQAGNQASHETSQDSGSALSAGQGLLSFIPFFSLGAEDS
                     YGFEGTQSWRGTFVARMSAVVKEVMPGGLLVIEGSRSVDVNGEKQELTLTGTVRAMDI
                     SRANTISSQQIADARIRYKGAGPMGQKAKPGILTRLLDFLF"
BASE COUNT          659 a         1356 c         1416 g          801 t
ORIGIN      
        1 accgaggagt cctcctgtat gagtgatgtt atactaacat agctactcag ttcgagcagc
       61 aaggcgtctc tgcgatccat caggtcgttg ggcacttggt ctgaggcgtt gaccttctgg
      121 atctcgtggt tcacggcggc gaggtcggca ccgatccggt tgacggtgtc gaccatgagc
      181 tgaatgtcac catccagctg ttcccgaatg gccgtcagag ccgcgtcgat gcgtccgaac
      241 tccgacgcca gtgtgctggc cgcctcgcgc agtgccgtcc ggttggcggt ctcttcgggc
      301 gagaggctga ggtcgtgcca tgccgagaag aagtcggtca ggagttggtc cagcgaggca
      361 tccgaggaga cgtcgacgat accctcgagc tgtgagtagg tatcggccag cacgtcgagt
      421 cgcgcctgcc gcgacgtcga tccgcgtatt tggtttgtga tgtactcgtt ctgtgcacgt
      481 gcaatcgtct cggccatcac accggtgcct agctgccctg ccatgttgta gggcgcggtg
      541 gtgggcgtcg cgaaggggct cgaggacacc aggtacaggc tttggcggga atagccctcg
      601 gtgctctgat tggccaggtt gtgcccgacc acgtcggtgc cggctcgctg ggccatgatg
      661 gcccgcttgc ccagttctat cccgaagaac gtcgacctcg tcatcatgag tcatcgcacc
      721 tcgcgttcgt ggcgaacacg ggcgctgtgt ccgactagac ctggcagtcc accacgccct
      781 gagtcgcgcg cctcggtcgg cctggcggca cgtaacgccc gtcggccctc agctgagctt
      841 cggtgagcac cgcaatggcg tggcgcgtgc tcgccaccgc atggcgcacc agggcctcat
      901 tgcggcggct gctactggcc agatccccca tggtccgggt catatcgccc agtagagcga
      961 gggccctctg ggcgtctgag gccggaaggg accgcgcgat ctcacgcaga gccgcatcgg
     1021 gtggaagccc caaagcgcca gccagcttgg ccgaagcggc ctgtcgccgt tcttcggcgc
     1081 actcgagacg agaggcggcc gcttcctgaa ggcgaaccag ggggcggatc cgatccggct
     1141 ggcccgtcac cagggcttcc tcctgagccc ccatgaggct gaggagctca cgaagccccg
     1201 ccagctgttc ctcgaggatc cgctcgagct ggtggaacag gtctgccaca ggtggcacag
     1261 ggcacctctc gtccccggct cttgccgcac ggacgttggc ggaagcgatc ttggccaggt
     1321 cagtgatcgg ccgcgtgtag cccgatggag acggccggat cggccgccgc cggcgggcta
     1381 ctcgtcgtcg tcggacagca gcagttcagc gatctcctcg gagtccacct tgtaggtgcc
     1441 gtcctggatc cgctggcgca gatcggccac caggtcctct cggacctcgg gagtctcttt
     1501 gagcgcccgc aaggcccggt gcacctcctg cgcctcctgc gagaggacga cttcgtccga
     1561 cttgcggacg accgtggatt cggccggctt ggccacctcc gcgcgcgcta cgtcttcggt
     1621 tcgcgacgtg cgctgagcga aggatcggag tagatcctgt acattccggt tcgtgactag
     1681 catccccgac gccccctaga gggctggccg gtcccgaaga cctcagaagc cgcgcctcgc
     1741 aacgagttgc tgatgcctgg ccaccaagtg gcacgaacgt ggatgaacgc gacgcagtac
     1801 cgttccggtt cagaacgaac cggctgtggc cggaagttcg ccgatgcagc ctgccacatg
     1861 cgggcctcct gcttcgatgg agcaccaggg cacacttcgc cctcacggtc ctgtcgcgtt
     1921 ttgccacttg ttctatcggc acattggcga ggcctcttga gcccattggg accggaatct
     1981 ttgcttgttg ggccgtcgtg gtgtgacggc gggcctggca gggtctccgc gggggtttcg
     2041 cggcacccgg aacacggaat ccctagcgtt gttcggccac aagtgcgccc ggaggcgcag
     2101 agtcctcact ctctacctgc gaggccattt ggtcgtagat catcttggcc aggccaatcc
     2161 cacctgcctt ggcgagctcc ctggtgagct cctggtccag cataccctcc cacatctcct
     2221 ccgcccgtcc gccgtggaac atctcgactt tgtggatggt cttgcgcatg ctctggacaa
     2281 gttggtacag aaggaatgac tcaaactcct gggcggcggc ccgtaggccg gccgaggtct
     2341 cgcccagctt ccgcaagctg ttcaggtctg cgacgttcag cgctgaggcc ggcacgtccc
     2401 ccgccccgag aagctccaga gccataccca tcactcccct gacgtgggtc cagtcgccta
     2461 ctgcatctcg atctccgcca gaatggctcc ggcggacttc atggcctgta ggatcgcgat
     2521 catgtcgcgc ggcgtggcgc cgatggcgct caatgacgag atgacatcgt caatggtatc
     2581 gccccccatg accggcatga gccgggactg gccttcggtc acggtcagtt tggtcttggt
     2641 tccggcgacg gtctgccctg acgagagcgg tggcggctgg gagacgacgg gcgtggctga
     2701 gacctggacg ctcaggttgg cgtgggccac ggccacgggc tggacgcgca cgttgccccc
     2761 catgacgacg gtgccggtct tctcgttgat gacgatcttg gccggtgtat cgacggtaag
     2821 ttcgacggtc tcgatctggg cgatgagatc gatgaggctg aactcggcat ctggcgggag
     2881 gctgacgcgg atcgacgagg cgtcgatggc gcgtgccgac gcccgaggga tcgcctcgtt
     2941 gatggcggcc gccgccctcg aggcggtaga gaagtcggcg tggctcaggg tgacggtgag
     3001 ggaccgctct tgcaccatct ccatctcgac ttctttctcg acgatggcgc cattggggat
     3061 ccggccggcg gtggggtggt tcttggtcac ccgctccccg ccgccgccgc ccgtggcatt
     3121 gaacccaccg atggtgatgg gcccctgcgc tacggcgtag acgtcgccat ccgcgccctg
     3181 cagaggcgtc tggatgagct gaccgccctg gaggctcttg gcgttgccga tggacgacac
     3241 cgtgacgtca atgcggtcgc ccggcttcac gaaggcgggt agctcggcgg taaccatgac
     3301 cgtcgccacg ttctgggtgc ggacctggcc ttcgacgttg acgccaagct gagctaacag
     3361 gttgagcagt gcctgactgg tgaagacggc ctgttgggag tcgccggtcc catcaagccc
     3421 gacgacgatt ccgaggccaa agagctggtt cgaccgcacg ccctggactt tgccgatgtc
     3481 tttgagccgg gtgcgagggc cggtcgtgcc ggacccgatc ggcgcctgcg cgaggctcgg
     3541 agcggtcgcg gccacgagca gcagtgcggt ccagacatga cacacgcgcc aacgcagcaa
     3601 cgctatcgcc ccctagaaca ggaaatcgag cagccgggtg aggatccccg gcttggcctt
     3661 ctggcccatg ggccctgcgc ctttgtagcg gattctggcg tccgcaatct gttgagagct
     3721 gatggtgttg gctctcgaga tgtccatggc gcgcacggtc ccggtcagcg tgagctcctg
     3781 cttctcgcca ttgacgtcga cggaccggga gccctcgatg acgaggagcc cgccgggcat
     3841 gacctctttg accacggcgg acatgcgggc cacgaaagtg ccgcgccagc tctgggtgcc
     3901 ctcgaagcca taactgtctt cggcacccag gctgaagaat gggatgaaac tgaggagtcc
     3961 ctggccggcg ctgagtgcac tgccgctgtc ctgactggtc tcgtgactcg cctgattgcc
     4021 ggcctgggtc gtctcgctga tgagtatggt gacgacgtcg cctacctggc gggcgacggg
     4081 atcggcgatg agcgtgcggc tgcccttggg gctgagcccg tcgggggcga cccagagaga
     4141 gcgggcgctg gcgccccgag cgacggcgag tgcggccgcg caggccagca gtgccggtac
     4201 tgcgcgatgg gatctcacga tgcgtctcct tc
//
LOCUS       DAOSPL010000180         4231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21002_length_4231_cov_3.158764, whole genome shotgun sequence.
ACCESSION   DAOSPL010000180 DAOSPL010000000
VERSION     DAOSPL010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21002_length_4231_cov_3.158764"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="PLQ54_04640"
     CDS             <1..1239
                     /locus_tag="PLQ54_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase"
                     /protein_id="HOS92575.1"
                     /translation="LGVSRTAVVVGHFEAQVRHALADLEGICFVRQPQPTGTADGVLL
                     GWSALDDADVLVAYGDTVFGGQELASMADGLADGVAAAALIAPLGAEQPNDWIIAHAD
                     GESLREVVGHDRGGTHRLGGLFALTRQARAYLERNPGIFRNVPVGGMPPVEADLGQSL
                     QMMMEDGLPVRACETRGLFVDLDRPWHIEQANVQYLEYLASQSDGNVVGEGSRVSDGA
                     EISGRLHLGRDCVIGNRVVIRGSLFLGDGSRVENGAIIDGHFSAGRGCRIRDYCAIGG
                     GSAIGDRAVVAHGAELSGVLFERVYLYHYCEMAGVYGSAVDIGAATVCGTLRFDDRTQ
                     VHHVRGRAEVPAWGGNGSYMGDYSRTGVNAMLMPGCKVGCHSCVGPGVIAYEDVPSRT
                     VVLAKQELTTRPWGPEKYGW"
     gene            1242..4049
                     /locus_tag="PLQ54_04645"
     CDS             1242..4049
                     /locus_tag="PLQ54_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92576.1"
                     /translation="MSVRRTRPDAARVAAGRVWGGAAALLALAWAQAHASAMLVDGFD
                     GTSLGRDWTADMALGSAVSVEDGRLHITARENTFAHIERPVGSDLITVSALWEPGTGT
                     GASWCSSVFLYWAPGDWVQLGVLDRGGGHYYAVETGGGGTTETDLAACDLTRRHWLRI
                     ELGRDVVRYQARPEGHPQWRTLRVIGRPQGLAGAPKLLVLGKGYGRGTAPYAAPDLDN
                     DYADPGPVVEAWFGEVRVQPTPASRQRLRPGEITPASDVEGDAILARPGDPVYDEVAS
                     VFPPLQHVREALGTKGHPDEFAVTEVGSLELMGTNEKGESCAVYAQACIGAGLRPFGG
                     GGRVGDRRLLEGYMPVLIAKSTDGDLELELTVFGHSEGLSPDAPLWAYVRLQVTNRGA
                     QPATVPVRLDTPDAPGAWGAVEGQLSLAAGGSDALHARIAYRLVKGASFERIDRPCYD
                     AALEETRAFWAAELGRGMQIDVPDARLAEAWRAWLAYNYIDVDQMNGTFEPHDGSGFY
                     EAVFAYSAARYAWVLDQYGRHDDAEAYLATLMAHQAPDGLLDWNSGLLDHGAYLAALA
                     EHYRLTGNTEWLRDVAPKAVAACDWVTRRRLVERGRLTGTPPVTYGLIRWRPYCDYFE
                     PVYSYVHNAYCCVGMERLADGLAEAGLTRDAQRIAADAALYRRDIMASMRRALFDLRG
                     RQVLPMEPDTHRLLKATQYRPKDYYSIIAPILLEAHFLPTESAEARALESFLREEGGT
                     ILGACEFGGGIDHAYGYGYMEHQLRLGRPERYILGLYASLAYGMSRETYSGVEVTMLR
                     TGDNALTLPHLYSGTHQLMMLRTMLVREEGDDLILCDAAPRDWLAPGKHIEVRGAESH
                     FGVVSFRIDSEWGRGRIVATVDPPARRRPSSIVLRLRVPEPRPIVSVTADGKPWKRFD
                     AECVFLPAGLGPVRVEVDYNR"
     gene            4046..>4231
                     /locus_tag="PLQ54_04650"
     CDS             4046..>4231
                     /locus_tag="PLQ54_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92577.1"
                     /translation="MTSARLVVGLALLSMMMSLTPSWAGGTMRRPDRIGVTAADLDLS
                     RTGQGPRAGRSAVCVEDA"
BASE COUNT          621 a         1430 c         1515 g          665 t
ORIGIN      
        1 ctcggcgtgt cgcgcaccgc ggtggtcgtc ggccacttcg aggcccaggt gcgccatgcg
       61 ctggccgatc tcgagggcat ctgcttcgtc cgccaacctc agccgaccgg caccgctgat
      121 ggggttctgc tcggctggtc ggctctcgac gacgcggacg tgctcgtggc ctacggcgac
      181 actgtgttcg gcggccagga gctggcctcc atggccgacg ggctcgccga tggagtcgcg
      241 gccgcggccc tgatcgcgcc cctcggcgcg gagcagccca acgactggat catcgcccat
      301 gccgatggcg agagcctgcg cgaggtggtc gggcacgatc gcggcgggac tcaccgcctg
      361 gggggcctgt tcgccctcac gcggcaggca cgggcctacc tcgagcgcaa ccccggcatc
      421 ttccgcaacg tgcccgtcgg gggcatgccc cccgtcgagg ccgacctcgg ccagtcgctg
      481 cagatgatga tggaggacgg cctgcccgtg cgtgcgtgcg agacccgagg gctgttcgtc
      541 gatctcgacc ggccctggca catcgagcaa gcgaatgtgc agtacctcga gtacctggcc
      601 tcgcagagcg acggcaacgt cgtgggcgaa ggctcgcgcg tcagtgatgg cgccgagatc
      661 agcggccgcc ttcacctggg gcgcgactgc gtgatcggca accgtgtggt gatccggggc
      721 agcctcttcc tcggcgacgg gagtcgcgtg gagaacggcg ccattatcga cgggcacttc
      781 tcggccggcc gcgggtgccg gatccgcgac tactgcgcca tcggcggcgg ctcggccatc
      841 ggcgatcgcg cggtggtcgc ccatggagcc gagctctccg gcgtgctgtt cgagcgcgtg
      901 tacctctacc actactgcga gatggccggg gtctacggca gcgcggtcga catcggtgcg
      961 gccacggtgt gtgggacctt gcgcttcgac gaccgcaccc aggtccacca tgtgaggggc
     1021 cgggccgagg tgcccgcatg gggcggcaat ggctcctaca tgggcgacta ctcgcgcacc
     1081 ggtgtcaacg ccatgctcat gccgggctgc aaggtcgggt gccacagctg cgtcggcccg
     1141 ggcgtcatcg cctacgagga tgtgcccagc cgcaccgtgg tcctcgccaa gcaggagctc
     1201 acgacccggc cctgggggcc ggagaagtac gggtggtagg catgagcgtg cgaaggacca
     1261 ggcctgacgc cgcccgcgtg gcggcggggc gggtttgggg cggagcggct gctctcctgg
     1321 cactcgcctg ggctcaggca cacgcctcgg ccatgctcgt cgacggcttc gacgggacct
     1381 cgctggggcg ggactggacc gccgacatgg ctctgggtag tgcggtgtcg gtcgaggacg
     1441 gacgcctgca catcaccgcc agggagaaca ccttcgcgca catcgagcgc cccgtcggca
     1501 gcgacctgat cacggtgtcg gctctctggg aaccgggaac cggtacgggt gcgagctggt
     1561 gctcgagcgt gtttctctac tgggcgccgg gcgactgggt ccaactcggc gtgctcgacc
     1621 gtggaggcgg ccactactat gccgtcgaga ccgggggcgg cggcacgacg gagacagacc
     1681 tggcggcgtg tgacctgacg cggcgacatt ggctccgcat cgagcttggg cgcgacgtgg
     1741 tccgctacca ggcgcggcca gagggtcacc cgcagtggcg gacactgcgc gtcatcgggc
     1801 ggccccaggg gcttgccggc gcgccgaagc tgctggtgct cggcaaaggg tatggacgag
     1861 gcacggcccc ctacgccgct ccggatctgg acaacgacta tgccgatccg ggtccagtgg
     1921 tggaggcgtg gtttggcgaa gtgcgcgtgc agccgacacc cgcttcgcgc cagcgcctgc
     1981 ggcctggtga gatcacgccc gcgagcgacg tggagggcga cgcgatcctc gcgcgccccg
     2041 gcgaccccgt ctacgacgaa gtcgcgagtg tctttccgcc gctgcagcac gtccgcgagg
     2101 ccctgggtac gaagggccac cctgacgagt tcgccgtcac cgaggtcggc tcgcttgagc
     2161 tcatgggcac caacgagaag ggcgagtcgt gcgccgtgta cgcgcaggcc tgtatcggcg
     2221 ccggcttgag gccattcggt ggaggcggcc gggtgggcga ccggcgcctg ctcgagggct
     2281 acatgcccgt gcttatcgcg aagtccactg acggtgacct cgagttggag ctgaccgtct
     2341 tcggccactc ggaggggctg agcccggacg cgccgctctg ggcctacgtc cgcctgcagg
     2401 tcaccaaccg gggcgcgcag ccggcaaccg tgccggttcg gctcgacacg cccgacgcgc
     2461 ccggcgcgtg gggcgccgtc gaggggcagc tctcattggc cgccggaggc agcgatgcgc
     2521 tgcacgcgcg catcgcgtac cgcctggtga agggagccag cttcgagcgg atcgaccgac
     2581 cgtgctacga tgctgccctg gaggagacgc gggccttctg ggccgccgag ctcgggcgcg
     2641 ggatgcagat cgacgtcccc gacgcgcgtc tcgccgaggc ctggcgagcg tggctggcct
     2701 acaactatat cgatgtcgac cagatgaacg gcaccttcga gccccacgac ggctccgggt
     2761 tctacgaggc cgtgttcgct tacagtgcgg cgcgctacgc ctgggttctc gaccagtacg
     2821 gccggcacga cgatgccgag gcgtacctgg ctacgctgat ggcgcaccag gcacccgatg
     2881 gactccttga ctggaattcg ggtctcctcg accacggcgc gtacttggcc gccttggccg
     2941 agcactaccg gctgacgggt aacacggaat ggctgcgcga cgtggcgccg aaggcggtcg
     3001 ccgcctgtga ctgggtcacg cggcgccggc tggtcgaacg cggccggctc acggggacgc
     3061 cgccggtgac ctatgggctc atcaggtggc gcccctactg cgactacttc gagccggtct
     3121 actcctacgt gcacaatgct tactgctgtg tggggatgga gcggctggca gacggcttgg
     3181 ccgaagcggg gctaacgcgc gacgcgcagc gcatcgctgc cgatgccgcg ctctaccggc
     3241 gggatatcat ggcctcgatg cgacgagcgc tgttcgacct gcgcggccgc caggtgctgc
     3301 ccatggagcc cgatacacac cgtctgctca aggccaccca gtaccgaccg aaggactact
     3361 acagcatcat cgcgcccata ctactcgagg cccacttcct acccacggag agtgccgaag
     3421 cgcgggccct ggagagcttc ctgcgcgagg aaggcgggac gatcctcggc gcctgtgagt
     3481 tcggtggcgg catcgatcac gcctatggct acggatacat ggagcaccag ctccgcctgg
     3541 gccggcccga gcgctacatc ctcggcctct acgccagtct ggcctacggc atgtcgcgcg
     3601 agacctactc gggcgtggaa gtgaccatgc tccggacagg cgacaacgcg ctcaccctcc
     3661 cccacctcta ctcgggcacg caccagctca tgatgctccg aaccatgctc gtccgtgagg
     3721 agggggatga cctgatcctg tgcgatgcgg caccgcgtga ctggctcgcg cctgggaagc
     3781 acatcgaggt gcgcggagcc gaatcgcact ttggcgtggt gagtttccgc atcgattccg
     3841 agtgggggcg gggccgcatc gtggcaaccg tcgacccgcc ggcccgcagg cggccatcga
     3901 gcattgtcct gcggctccgg gtgccggagc ccaggcccat cgtgtcggtg accgccgacg
     3961 gcaagccctg gaagcgcttc gacgccgagt gtgtcttcct gcccgcgggc ttgggtccgg
     4021 tgcgagtcga ggtggactac aaccgatgac ctcggctcgc ctggtggtag gactggctct
     4081 gctctccatg atgatgtcac tgacgccctc ctgggccggc gggaccatgc gccgaccaga
     4141 ccgcatcgga gtgaccgctg cggatctgga cctgtcacga acgggccagg gaccccgggc
     4201 cgggcgctcg gccgtatgtg ttgaggacgc a
//
LOCUS       DAOSPL010000181         4229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21012_length_4229_cov_3.296598, whole genome shotgun sequence.
ACCESSION   DAOSPL010000181 DAOSPL010000000
VERSION     DAOSPL010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21012_length_4229_cov_3.296598"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PLQ54_04655"
     CDS             complement(<1..279)
                     /locus_tag="PLQ54_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOS92578.1"
                     /translation="MMRRMRGWWVWAAAAALSLCLLSAAPERTAHLVVLHINDTHGAL
                     LPETRADGSVAGGVARVATLARQVREQAPGRVLFLHAGDVLSRGGPLTV"
     gene            458..1576
                     /locus_tag="PLQ54_04660"
     CDS             458..1576
                     /locus_tag="PLQ54_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS92579.1"
                     /translation="MGAVIARGAWAQATDKKVRIGVVGGGFGASFQWHLDPNCIVAAV
                     SDLRTDRRDYLTQVYQCPTAYESLEKLILDDSLDAVAVFTGAPDHVRHCVAVMDTGKH
                     CICAVPAATSLEECEQLIDAKVRNNVRYMMAETSYYRWPTITMRELYRDGKFGEVLYS
                     EVEYYHPMNDAERNGLWFYNGERTWRYGYPPMLYPTHSTGFLVGVTRERLTKVSCLGY
                     GAPDGPGLSDNAYNNPYSCQAAIFRTSGGHICRCNVMWAIWAHGERAQWMGARMSAFM
                     DGWAGQPYVLKSVDGPDVLGDPNYFERLPEAMRAESGHGGSHPFLTHEFVTALVEDRE
                     PAIDVYESVAMTAPGIVAHQSAFKNGEQLEVPSFDPER"
     gene            1616..3298
                     /locus_tag="PLQ54_04665"
     CDS             1616..3298
                     /locus_tag="PLQ54_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92580.1"
                     /translation="MVSLGALGVLSTSASAAPLAGWSVLSGEGWDQVVDLQTMLLQRE
                     GQGVAAAVSDGIHGGSGAWRCVVEPSLGAQACGIWFQATKDLSAGLRAELGPPGTVGF
                     ALKDARGEVLWQDAWAPWAPYQAYVIEGIVEGDRARVQLLAYDKRTLVSQSDFVALPE
                     GAVAADGHLAVYTENGIARFWGAERADEPLAALTEDAPNRRRLAEGPNPDWALFGTGN
                     WAWTDATRTRIRQSAMAERAWAVDKTSRATRNTWQTWVRVSPGAGGAGIAFPTDDSAN
                     GGMMAWLGGNFGAGGLMLYTNSGPTGVGEALWSSPQDMWHYDEDLLIRAEAKGTEVRI
                     QLLAADGTTVITESPWIAVPADRIGHEGIMAFHTWKGSAEFWGFRQGEGPAGPAGPTA
                     SALGEGWLQAGGAWEWTDEARSALKQSGVDEVASCVHTAVRAARATWQCRVAITSTQG
                     GAGLLAQVSPALDQGFACLILPGRAEMVDLGQKRTLWSADGLHWEAGAEYLVQAIVQT
                     DRVRMRVCAADGQTVIAESPDVYIPDTNNDRIGHLGFTCRGGSAEFSGWGVH"
     gene            3298..>4229
                     /locus_tag="PLQ54_04670"
     CDS             3298..>4229
                     /locus_tag="PLQ54_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HOS92581.1"
                     /translation="MPGPSGDLIVLWPEGAPGAVGTADDDIPALTPYVVDAPGRARSA
                     VVVCPGGGYAGLAPHEAEPVALWLNALGVSAFVLRYRLAPKYRYPSAHSDASRAIRTV
                     RARAAEWDIDRAQIGILGFSAGGHLSSTVGTHFDAGNPEAADTIERASSRPDFMILIY
                     PVISLKNYAHAGSRANLLGDPADPDLVNYLSSEEQVTAETPPTFLIHTAGDTGVPCEN
                     SLLFAMALRRAGVPLELQSNTADPDITRKSRASESEVYAQIVADLDLAAKNLPDSYGS
                     SANVNKVRATKGAANALLAKVWAQRSDRDYTHVL"
BASE COUNT          701 a         1466 c         1406 g          656 t
ORIGIN      
        1 gaccgtcagc ggcccgccgc gcgagagcac atcgccggcg tgcagaaaca gcacccgccc
       61 gggagcctgc tcccgaacct gacgcgccag cgtcgccacg cgcgccacgc cgcccgccac
      121 cgacccatcg gctcgcgtct cgggcagcag cgccccatgc gtgtcattga tgtgaaggac
      181 caccaggtgg gcggtccgct cgggcgcagc gctgagcagg cagagggaca gcgccgcggc
      241 cgcggcccag acccaccacc cgcgcattcg tcgcatcatc cagtcaccag ctccggcaag
      301 ccgattcggt gcacgctacc cagctccttt ggagggccac cacccgtgcg gaccgaacta
      361 gcccccgccg cgctctgcgg ctgagggggg atccgcgttg tcccagaacg ggaatgtctc
      421 ccgtcggaac tttctcaaag cggtaggagg gggcgccttg ggagccgtca tcgctcgcgg
      481 tgcatgggct caggccaccg acaagaaggt ccgcatcggc gtggtgggcg gcggcttcgg
      541 cgcctccttc cagtggcacc ttgaccccaa ctgcattgtg gccgccgtga gcgacctgcg
      601 gaccgaccgc cgcgactacc tgacccaggt ctaccagtgc cccacggcct atgagtccct
      661 cgagaagctg attctggatg actcgctgga cgccgtggcc gtcttcaccg gtgccccgga
      721 tcacgtccgg cactgcgtcg cggtgatgga cacgggaaag cactgtatct gcgccgtgcc
      781 cgcggccacc tctctggagg agtgcgagca gctcatcgac gccaaagtgc gcaacaacgt
      841 ccgctacatg atggccgaga ccagctacta ccgctggccg accatcacca tgcgcgagct
      901 ctaccgcgac ggcaagttcg gcgaggtcct gtactctgag gtcgagtact accacccgat
      961 gaacgatgcc gaacgcaacg ggctgtggtt ctacaacggc gagcgcacct ggcgctacgg
     1021 ctacccgccc atgctctacc ccacccactc caccggcttc ctggtcggcg tcacccgcga
     1081 gcgcctgacc aaggtgtcgt gcctgggcta cggtgcgccc gacggaccgg ggttgtccga
     1141 caatgcctac aacaacccct actcgtgcca ggccgccatc ttccgcacca gcggcggcca
     1201 catctgccgc tgcaacgtca tgtgggccat ctgggcccat ggagaacggg ctcagtggat
     1261 gggggctcgc atgagcgcct tcatggatgg ctgggcgggc caaccctacg tactcaagtc
     1321 cgtcgatggg ccggatgtgc tcggcgaccc gaactacttc gagcggctcc ccgaagccat
     1381 gcgagccgag agcggccacg gtgggtccca cccattcctc acccatgagt tcgtcaccgc
     1441 attggtcgag gatcgcgagc ccgccatcga tgtctacgag tccgtcgcca tgacggcccc
     1501 cggcatcgtc gcccatcagt cggccttcaa gaacggcgag cagctcgagg tcccgtcctt
     1561 cgatccggag aggtaggtcc atcccatgtt cctgttgcga ggaggccggc ttctcatggt
     1621 cagcctgggt gctcttggtg tgttgtcaac cagtgccagt gccgcgccgt tggcaggctg
     1681 gtcggtcctt tcgggggagg ggtgggacca agtcgttgac ctccagacca tgctgctcca
     1741 gcgagagggc cagggcgtgg cggccgcggt cagcgatggc atccacgggg gatccggggc
     1801 ttggcgctgc gtagtcgaac cgtcattggg tgcccaggcc tgtggcatct ggttccaggc
     1861 tacgaaggat ctctcggccg gcctccgcgc cgagctcggg cccccgggca cggtgggctt
     1921 tgctctgaag gacgccaggg gtgaggtgct gtggcaagac gcatgggcgc cttgggcccc
     1981 ctaccaggcc tacgtcatcg agggcatcgt cgaaggggat cgagcccgcg tgcagctctt
     2041 ggcctatgac aagaggaccc tggtctcgca gagcgacttt gtggcacttc ccgagggcgc
     2101 ggtggcagcc gacggccatc tcgcagtcta caccgagaac ggaatcgctc ggttctgggg
     2161 tgccgaacgg gccgacgagc ccctcgcggc gctgaccgag gacgccccga accggaggcg
     2221 cctggccgag ggaccgaacc ccgactgggc cctgttcggc accggcaact gggcgtggac
     2281 cgatgccacc cgcacccgca tccgccagtc ggccatggcc gagcgggcgt gggctgtgga
     2341 caagaccagc cgagcgacgc gcaacacgtg gcagacttgg gtgcgcgtat ctccgggcgc
     2401 gggcggggcg gggatcgcct tccccaccga tgatagcgcc aacggcggga tgatggcctg
     2461 gctcggcggc aacttcggcg ctggcggcct catgctctac accaacagcg gacccacggg
     2521 agtcggcgag gccctctggt cgagcccgca ggacatgtgg cactacgacg aggacctgct
     2581 catccgtgcc gaagccaagg gcacagaagt ccgcatccag cttctcgccg ccgatggcac
     2641 caccgtcatc accgagagcc cctggatcgc cgtgcctgcg gaccggatcg gccacgaggg
     2701 catcatggcc ttccacacct ggaaggggag tgcggagttc tggggtttcc gccagggaga
     2761 gggcccggcg gggccggccg gacccacggc atcggcgctg ggcgaaggct ggctccaggc
     2821 aggcggggcg tgggagtgga cagatgaagc caggtcggcc ctgaagcagt ccggcgtcga
     2881 cgaggtcgcc tcgtgcgtcc atacggcggt ccgagcagct cgggcaacgt ggcagtgccg
     2941 cgtggcgatc acgagcaccc agggcggggc cggcctgctg gcgcaggtca gcccggcact
     3001 cgaccaaggg tttgcctgcc tgattctgcc cggtagagcc gagatggtcg acttgggtca
     3061 gaagcggacg ctctggtcgg ccgacggcct tcactgggag gccggtgcgg agtacctcgt
     3121 gcaggccatc gtccagacag accgtgtccg catgcgcgtc tgtgctgccg acgggcagac
     3181 ggtgatcgcc gagagcccgg atgtgtacat cccggacacg aacaacgacc gcatcgggca
     3241 cctgggcttc acctgccggg gcggttccgc ggagttctca ggttgggggg tgcactgatg
     3301 ccgggaccaa gtggtgacct catcgtgcta tggccggagg gggcgccggg tgccgtcggg
     3361 accgccgatg acgacatccc ggccctcacg ccctacgtcg tcgacgcccc cggtcgagct
     3421 cggtccgccg tggtcgtgtg cccgggtgga ggctacgcag gcctcgcgcc ccacgaggcc
     3481 gagcccgtcg ccttgtggct caacgccttg ggcgtgtcgg cgttcgtgct gcgctaccgc
     3541 ctggccccca agtaccgcta tccgagcgcc cacagcgacg cgtcgcgcgc catccggacc
     3601 gtccgcgctc gagcggcgga gtgggatatc gatcgcgccc agatcggcat cctgggcttc
     3661 tccgccggcg gacacctgtc atccacggtg ggcacccact tcgacgccgg gaacccggag
     3721 gcagccgata ccatcgaacg ggccagctcc cggcccgact tcatgatcct catctacccg
     3781 gtcatctcgc tgaagaacta cgcccacgcc ggctcccggg ccaacctgct cggcgacccg
     3841 gccgatccgg acctcgtgaa ctacctctcg agcgaggagc aggtcacggc cgagacgccg
     3901 ccgaccttcc tcatccacac tgccggagac accggcgtgc cctgcgagaa cagcttgctg
     3961 tttgccatgg cgctgcgtcg tgccggtgtt ccgctcgaac tgcaatccaa tacggccgat
     4021 ccggacatca cacgcaaaag ccgcgcttcg gaatcggagg tgtatgccca gatcgtggca
     4081 gacctcgacc tggcggccaa aaacttgccc gattcctacg gaagcagcgc aaacgtcaat
     4141 aaagtgcgtg ctaccaaagg cgctgccaat gccctgctgg caaaagtatg ggcacaacgc
     4201 agcgaccgcg attacaccca cgtgctgga
//
LOCUS       DAOSPL010000182         4222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21060_length_4222_cov_3.296856, whole genome shotgun sequence.
ACCESSION   DAOSPL010000182 DAOSPL010000000
VERSION     DAOSPL010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21060_length_4222_cov_3.296856"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1737
                     /locus_tag="PLQ54_04675"
     CDS             <1..1737
                     /locus_tag="PLQ54_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92582.1"
                     /translation="VVGDGPLWEGRVASTGSALADLVLQPVEPVRFDDDFMRGEDQEG
                     PWEVVSGEWATKAEGDPKMGVNPFNYQGQARGVGLATAGWPFWDEYEFSVSARPTQAG
                     TVGQALYYQDAANHLLFRARVSDTPNDAAGGLELVRVKGGQERIVAQGDGCLVTGHWY
                     RLTARVLGGTVSAALDGQWVLWADENSFPGGRVALYLKDCRSEFDDVEVRAATGEEAP
                     ALVELDGSVPPFAGTLDRDTWAGTALQWRPVREEPGLFWRRGRFHGDFELSFQCNFGE
                     ARAADPSLTLLLTSELEAPGDGFAIAVAPRRGERECSVELTGRGRRRRTTVTASASPV
                     VTVRRTGGALTALLDGRPLPDIPADEAAGELSCLGFRAQGFRPRLSGLELRAGNVLDD
                     CFDRAPTPWWVSSGEWAISSRWPCTAEWSWFGGRSKEVAAIWHKRRFEGDIIVDSHIG
                     PWEIDHGDGTPREICRAFNLVLCGDGEDANSGYSFVLGADEAGSGATLSRAGRVVARN
                     DEYRIYSDAHNQWLNIRAEKRGRQVGIWIADQPILVWEDPEPLPSGRVGIWTRDNGIM
                     IPRVTIYYQAMP"
     gene            complement(1802..>4222)
                     /locus_tag="PLQ54_04680"
     CDS             complement(1802..>4222)
                     /locus_tag="PLQ54_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92583.1"
                     /translation="LVVGSVFDLAPTECRISDDTPWRKALSLRGRSALHGYEWECRVE
                     ASDATPLLHFVITIHLPDGLSLPEPQPTVAMWMPGAADAVTVGQGPESIYGPDAPILG
                     FPAAYLWHAGAEAVVFFDMTPMTWMSRGGVDRFLDCRVEARERGGSLGLGLHTWKLTG
                     NAVPPGDMVLSLYLYEARRPARPTKLEALDTLARACAPLHPASAPSIAWPGGSPPPWG
                     QIAAGTLRDLMIPGVTYEEQEAGWRDAPLELVPPVDRMVVHPDRPRPPSAEGPMPWDF
                     STVNNHLSAWILYNRLRPDEPLGPELVLRKKDALPLFYDPETRLIRWGTRTPPHVAGP
                     DMAWQCLFFHHETMRVYDALPPEAFSPAIAGRFLMATRGLTALAHNTQYVIPQWFDPE
                     RKVPSVQHDVPELGAVREPWQVGTYAHLMLRAHEVSGDPAYLAEAQASLRALFEPMSY
                     SISNDRYERTYADPADFPITEIHGNALGIVAAYRLYEQTGNAVFAQYSRAFLNSLLRM
                     TFWYDDQTDAPSRSLSNLGLFLPFGGACSATPWETCEAHLALAWILKHDTQMPDDVRE
                     LLLRLSNLHRRSALLFYPPTWPDAVRELDPGATHEAWPYLPTEPFYSLEGGGHRGAPA
                     AYMAGSAVWCYWLYEALARSTDPDVLVLNLDVHEGFEEALAGHERHVYLYNPTPEPRD
                     VDVTFPGLPADVYALTVEGAAPSTAQHSSDELGTGVPIRLAARAWAPLTLHREDSAEA
                     RDRIAACKQARNALSRAYQLLQEAALSGATPAPSVELFGRAMEGYRRGDFGRARLMAQ
                     DSAKGLGP"
BASE COUNT          649 a         1347 c         1565 g          661 t
ORIGIN      
        1 gtggtgggcg acggtcctct gtgggagggg cgcgtcgcgt cgacgggctc ggccctggcc
       61 gacctcgttt tgcagcccgt ggagcccgtg cggttcgatg atgacttcat gcggggcgag
      121 gaccaggagg gcccctggga ggtcgtctcg ggcgagtggg cgacgaaggc cgagggtgac
      181 cccaagatgg gtgtgaaccc gttcaactat cagggccaag cccggggcgt cggtctggcg
      241 acagcaggct ggcccttctg ggacgagtac gagttcagcg tctcggcgcg ccccacgcag
      301 gcgggcaccg tcggccaggc gctctactac caggacgcgg cgaaccacct gctgttccgc
      361 gcccgggtga gtgacacgcc gaacgacgcg gcgggcggcc tcgagctggt gcgagtgaaa
      421 ggcggccagg agcgcatcgt ggcgcagggc gacggctgtc tcgtgaccgg tcactggtat
      481 cggcttaccg ccagggtctt gggtggtacg gtctcggcag ccctcgacgg ccagtgggtc
      541 ctgtgggcgg acgagaactc gtttcccggc ggccgggttg ctctgtatct gaaggactgc
      601 cgatccgagt tcgatgacgt cgaggtgcgc gccgcgacgg gcgaagaggc gccggccctg
      661 gtggagctcg atggcagtgt cccgcccttt gcgggtacgc tcgaccgcga cacgtgggcc
      721 gggacggccc tgcagtggcg acccgtgcga gaggaaccag ggctcttctg gcgccgaggc
      781 cgcttccacg gcgacttcga gctctcgttc cagtgcaact tcggcgaagc acgggcggcc
      841 gatccctcgc tgacactgtt actgacctcg gaactggagg cacccggtga cggcttcgcg
      901 attgccgtgg cgccccggcg cggggaacgc gagtgctccg tggagctgac agggcgcggc
      961 cgtaggaggc gcacgacggt gaccgcgtca gcctcgcccg tggtgaccgt gaggcgcacg
     1021 ggcggggcgc tcaccgcgct ccttgatggt cgcccgttgc cggacatccc cgcggacgag
     1081 gccgcgggcg agttgtcatg cctcgggttc cgggcccagg gtttccggcc ccggctctcc
     1141 gggctcgagc tgcgtgccgg gaacgtgctg gacgactgtt tcgaccgtgc gcccaccccg
     1201 tggtgggtct ccagtggcga gtgggccatc tcgagtcgat ggccgtgtac cgccgagtgg
     1261 tcctggttcg gtggccggag caaggaggtg gccgccatct ggcataagcg ccggttcgag
     1321 ggtgacatca tcgtggactc gcacatcggc ccgtgggaga tcgaccatgg cgatggcact
     1381 ccgcgcgaga tctgccgggc gttcaacctg gtactctgcg gtgacggcga ggacgccaat
     1441 agtggctaca gcttcgtgct gggagccgac gaagccgggt cgggcgcgac gctgtctcga
     1501 gccgggcggg tcgtggcgcg gaacgatgag taccgcatct acagcgacgc acacaaccag
     1561 tggctcaaca tccgggccga gaagcgcggg cgccaagtgg gcatctggat cgcggaccag
     1621 ccgatactcg tgtgggagga ccccgagccg ctaccctctg gccgggtggg gatctggacg
     1681 cgcgacaacg gcatcatgat cccgagggta acgatctact accaggcgat gccgtgagcc
     1741 actgagagcc gggcagatca gggcggcgcc tgccgctgtt gggacggaga agccgctgct
     1801 gtcaggggcc aagccccttg gccgagtcct gagccatgag cctggcgcgg ccgaagtcgc
     1861 cgcgtcggta gccctccatg gctctgccga agagctccac agatggcgcg ggtgtcgcac
     1921 ccgacaaggc ggcctcctgc agcagctgat acgcgcgcga cagggcgttg cgtgcctgct
     1981 tgcacgccgc aatccggtct cgcgcttcgg cgctatcctc gcgatggagc gtcagggggg
     2041 cccaggcgcg ggcggcgagc cggatgggta cccccgtccc cagctcgtcc gacgagtgtt
     2101 gcgcggtgga cggcgcagcg ccctcgacgg tcagcgcgta gacgtccgcc gggagcccag
     2161 ggaacgtgac atcaacgtct cttggctcgg gcgtcgggtt gtacaggtac acatggcgtt
     2221 catggccggc aagggcctcc tcgaagccct cgtgcacgtc caggttgagg accagcacgt
     2281 ccggatccgt cgaccgggcc agcgcctcgt agagccagta gcaccagacg gcggagccgg
     2341 ccatgtaggc cgcaggcgcg cctcggtgtc cccctccctc gagggagtag aacggttcag
     2401 tgggcaggta gggccaggct tcgtgggtgg cgcccgggtc gagctcgcgc acggcatccg
     2461 gccaagttgg cgggtagaac aggagggcgc tgcggcgatg gaggttcgat aggcggagca
     2521 gcagctctcg cacgtcgtct ggcatctgtg tgtcgtgctt gaggatccag gccagggcca
     2581 agtgggcctc gcaggtctcc cacggcgtag cgctgcacgc accgccaaag ggcaggaaga
     2641 gccccaggtt gctcagggac cggctcggcg cgtccgtctg gtcgtcatac cagaaggtca
     2701 tgcgcagcag gctgttcagg aaagccctcg agtactgtgc gaagacggcg ttcccggtct
     2761 gctcgtagag gcggtaggcc gcgacgatgc cgagcgcgtt gccgtgtatc tcagtgatgg
     2821 ggaagtccgc cggatcggcg taggttcgct cgtaacggtc gttgctgatg gagtagctca
     2881 tcggctcgaa caaggcccgg agggacgcct gggcctcggc caagtacgcg gggtcgcccg
     2941 acacctcgtg agcccgcagc atgaggtggg cgtacgtgcc cacttgccat ggttccctca
     3001 cggccccgag ctccggcacg tcgtgctgga ccgatggcac cttccgttcc gggtcaaacc
     3061 actgggggat cacgtactgg gtgttgtggg ccagcgccgt cagcccgcgc gtcgccatga
     3121 ggaaacggcc ggcgatggcc gggctgaacg cctcgggcgg gagcgcatcg tacacccgca
     3181 tggtctcgtg gtggaagaac aggcactgcc acgccatgtc gggtcccgcc acgtggggag
     3241 gcgtccgcgt cccccaacgt atgagacgcg tctcagggtc gtagaacagc ggcagcgcgt
     3301 ccttcttgcg gagcaccagc tccggcccca gtggttcgtc cgggcggagc cggttgtaga
     3361 ggatccacgc cgacaggtgg ttgttgacgg tggagaagtc ccatggcatg gggccttccg
     3421 cactgggcgg ccgcggccga tccgggtgga ccaccatccg gtcgacggga ggcacgagct
     3481 caagaggggc gtcccgccat ccggcctcct gctcttcgta ggtcacgccc gggatcatga
     3541 gatcgcgtag cgtcccggcc gcgatctggc cccatggagg aggcgaaccg cccggccacg
     3601 cgatcgaggg cgccgatgcc ggatgaagag gagcacaggc acgcgccagg gtgtcgagcg
     3661 cctccagctt ggtgggcctg gccggacgcc gtgcctcgta gagatagagg ctgagaacca
     3721 tgtctccggg cggtaccgcg ttaccagtga gcttccatgt gtggagcccg aggcccaggc
     3781 tcccaccgcg ttctcgcgcc tcgacccggc agtcgaggaa gcgatcgacg ccgccgcgag
     3841 acatccatgt catgggcgtc atgtcgaaga agacgacggc ctcggccccc gcgtgccaga
     3901 ggtaggctgc ggggaatccg aggatcgggg cgtccgggcc gtagatgctc tcagggccct
     3961 gcccgacggt cacggcatcg gccgccccgg gcatccacat cgccacggtc ggttggggct
     4021 cggggagcga caggccgtcc ggcagatgga tggtgatgac gaagtggagc agtggcgtcg
     4081 catcgctcgc ctcgacgcgg cactcccact cgtagccgtg gagcgcgctc cggccgcgca
     4141 ggctgagggc cttgcgccac ggcgtgtcgt cgctgatccg gcactcggtg ggcgccaggt
     4201 cgaaaaccga gccgacgaca ag
//
LOCUS       DAOSPL010000183         4222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21061_length_4222_cov_3.233981, whole genome shotgun sequence.
ACCESSION   DAOSPL010000183 DAOSPL010000000
VERSION     DAOSPL010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21061_length_4222_cov_3.233981"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="PLQ54_04685"
     CDS             complement(<1..722)
                     /locus_tag="PLQ54_04685"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOS92584.1"
                     /translation="MHAPLLACGAGEGSAGGAATGTDAALRAMLANSLQLPTIPHVAA
                     ALIRVADQPRASASDVRDVISTDQALVTKLLRVVNSPMYGMRKRVTTLTHAVAMLGFE
                     AVKNLALAASFSEVLGQSAEGYGLDPGRLWMHSIAAGCAARLAAERTGYAQPEEAFVA
                     GVIHDVGKLAASVHIQSMYSEILAKAACGKRAFNAVEREVMGFDHAQAGEAVALSWNL
                     PEPLSQSIRWHHEPGRAEHAVL"
     gene            complement(724..1191)
                     /locus_tag="PLQ54_04690"
     CDS             complement(724..1191)
                     /locus_tag="PLQ54_04690"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOS92585.1"
                     /translation="MPSRTRVEHGEVAVSNDPGDVLEARDLAACVAICMFDPIANVGG
                     LAHVVLPSSTIQDGPTRPAVFADRAVPLLIEAMTEKGAQPTHLKVTMAGGAEFIGATA
                     DDPVGGLGRRTVDAIDGLLRARRIDVTQRSVGGLGSRCVELRLGEGRVVVDPE"
     gene            complement(1268..2086)
                     /locus_tag="PLQ54_04695"
     CDS             complement(1268..2086)
                     /locus_tag="PLQ54_04695"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOS92586.1"
                     /translation="MRAGIRLPVLPHVAAAVIEVADSPRASADDLREAMSTDQALVAK
                     LLRVVNSPMYSPRQPVTTVTHAIAFLGFESVRNLALAASFSDVLTSGAAGYGLEPGQL
                     WSHSVAVGCATKLLSEAAKYPQPEEAFLAGVIHDVGKLVLSVNMEREYGGAFAGRGSG
                     DRPFDEAERACLGFCHAEAGAAVASDWGLPKLLCDAIRWHHSLDANGSGLLTHLVHVG
                     DAIGLVLGYGIGRDGLEYRFCRGALQALGVGDQQLAGLMAAVPRAVRRIAGSRT"
     gene            complement(2154..2621)
                     /locus_tag="PLQ54_04700"
     CDS             complement(2154..2621)
                     /locus_tag="PLQ54_04700"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOS92587.1"
                     /translation="MARRVLVEQSQVVVSNNPEEVLEARGIASCVAVCVHDARARAGG
                     IAYIMMPSSLVEDEGCPAGAFADRAVRTLMSQMATKEASSENCQITLVGGAEMLGATA
                     SDAVSGLGRRNIQAVESLLRTRRLGVANRHLGGHRARDVGLRIADGQIAVAER"
     gene            complement(2757..4055)
                     /locus_tag="PLQ54_04705"
     CDS             complement(2757..4055)
                     /locus_tag="PLQ54_04705"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOS92588.1"
                     /translation="MTTPVELIEAKKRGRSLSEGEIAALVDGVVTGRMSEAQAAAFLM
                     AVWFQGMSQEEVTGLTLAMARSGRMLDLSPLGFCVDKHSTGGVGDKTSLVVGPLVAAA
                     GGTVVKMAGRGLGHTGGTVDKLEAIHGMRVDLSVEEMLDVASRVGLVISGQTEELVPA
                     DRVLYAIRDVTATVDSAPLVASSVMSKKLAAGAHGIVIDVKYGVGAFMASAAEARALG
                     ELMARIGTDAGRSMAVALSPMDDPLGCAAGNALEVAEAVRCLKNEGPADVTQLCLELS
                     TRMLMLAGVASEAAEGRKAAERALASGQALERLVRMVEAQGGDPGCVEEPDRLPAAPW
                     RIPVRAQASGYVHQVDARQVGLACVAAGAGRERRSQAVDPAAGVLLRCRRGEEVRAGD
                     VVAEVHASDRPRAERAASMVSAAFRIGAGRPRATDALVWL"
     gene            complement(4052..>4222)
                     /locus_tag="PLQ54_04710"
     CDS             complement(4052..>4222)
                     /locus_tag="PLQ54_04710"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525016.1"
                     /note="catalyzes the transfer of phosphate between the C1
                     and C5 carbons of pentose; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOS92589.1"
                     /translation="REYVPVLVREPGCVVGRDLGTRPTLADVGATAAEWLGVERIGPG
                     TSLLAETDGSIA"
BASE COUNT          688 a         1618 c         1280 g          636 t
ORIGIN      
        1 tccagaaccg cgtgctcggc gcggccgggc tcgtggtgcc accggatgga ctgcgacagt
       61 ggctcgggca ggttccacga cagcgcgacg gcctcccccg cctgagcgtg gtcgaacccc
      121 atcacctcgc gctcgaccgc gttgaacgct cgcttgccgc acgcggcctt ggccaggatc
      181 tcggaataca tcgactggat gtggaccgag gctgccaact tgcccacgtc gtggatgacc
      241 cccgccacga acgcttcctc gggctgcgcg tagcccgtgc gctcggcggc cagccgcgcg
      301 gcgcaacccg cagcgatgga atgcatccac agccggccag ggtccagacc gtagccctcg
      361 gctgactggc ccaacacctc ggagaacgac gcggccagcg ccaggttctt cacggcctcg
      421 aacccgagca tggcaacagc atgcgtgagg gttgtcaccc gcttccgcat gccgtacatg
      481 ggtgagttga cgacgcgcag gagcttggtt accagcgcct gatctgtcga gatgacatcc
      541 cgcacatcgc tggcactggc ccggggctgg tccgccaccc gaatcagcgc ggccgccacg
      601 tgtggaatgg tcggcaactg caacgagttg gccagcatgg cgcgcagcgc ggcgtcggtt
      661 ccggttgccg cgccgcccgc acttccctct ccggccccgc aggccaagag cggtgcgtgc
      721 acgctactcg gggtcgacga cgaccctacc ctcaccgagt cgaagttcca cacagcggct
      781 acccaatccc cccacagagc gttgggtcac gtcgattcgt cgtgctctca acagcccgtc
      841 gatggcgtca acggtccggc ggccgagccc tcccaccggg tcatccgccg tggcaccgat
      901 gaactcggcc ccaccggcca tggtcacctt caggtgggtg ggctgggccc ctttctccgt
      961 catcgcctcg atgagcagcg gcaccgcgcg atcggcgaac accgccgggc gtgtcgggcc
     1021 gtcctggatg gtactgctcg ggagaacaac gtgagccagg ccgccgacgt tcgcgatggg
     1081 gtcaaacatg cagatcgcga cgcaggccgc caggtcgcga gcctcaagta cgtccccggg
     1141 atcattgctt acggccactt cgccatgttc cacacgtgtt cgactcggca tctgctttcc
     1201 ctccacccgg gcgccggtat ccatgcggca tcactgggcg cctcggtcac ctccgttggc
     1261 tggtgtgtca ggtccggctt cccgcgatac gtctcaccgc gcgcgggacc gccgccatca
     1321 ggcccgcaag ctgctgatct ccgacgccga gggcctgcag cgctccccgg cagaagcggt
     1381 actcgaggcc gtcacggcca atgccgtacc cgaggaccag cccgatcgcg tcaccgacgt
     1441 gcaccagatg cgtgagcagg ccactcccgt tcgcatccag gctgtggtgc cagcgtatgg
     1501 cgtcacacag cagctttgga agaccccagt ccgatgcgac cgccgcaccc gcttcggcat
     1561 ggcagaagcc caaacaggcg cgctcggcct cgtcgaatgg acggtcgccc gacccgcgcc
     1621 ccgcgaaagc tcccccgtac tcgcgctcca tgttcacact cagaacgagc ttgcccacat
     1681 cgtggatcac gccagcgaga aacgcctcct cgggttgggg gtacttggcc gcctccgaca
     1741 ggagcttcgt cgcgcacccc accgccaccg agtgcgacca caactgcccg ggctccaggc
     1801 cgtagcccgc cgcacccgac gtcagcacat cggagaaaga cgccgccagc gccagattgc
     1861 gcacggactc aaaaccgagg aaagcgatgg catgggtcac ggtcgtgacc ggctggcggg
     1921 gcgagtacat cggcgagttc acgacgcgaa gcagtttggc gaccagcgcc tgatcggtcg
     1981 acatggcctc gcggagatcg tcggcgctgg cccgcggcga gtcggcaacc tcgatcaccg
     2041 ccgcggccac atggggcagc accggcagcc ggatgcccgc ccgcaccagg cgatcgaccc
     2101 cgtgcgatgg cggcaccgtg cgcgccgagg cggatccctc gacggctgcg ccgctagcgc
     2161 tcggcgacgg cgatctggcc gtcagcgatt cgaagtccca catcccgcgc gcggtgcccc
     2221 cccaaatgcc gattcgcaac accaagacgc cgagtcctga gcaatgactc tactgcctgt
     2281 atgttccgcc ggcccaagcc actgacggcg tcggaagccg tagcgccgag catctcggcg
     2341 ccgccgacca gcgtgatctg gcagttctcg ctcgaagctt ccttcgtggc catctgcgac
     2401 atcagcgtcc gcacggccct gtccgcgaac gcccctgcgg ggcacccctc gtcttccaca
     2461 aggctactgg gcatcatgat gtaggcgatg ccccccgctc gcgcccgggc atcatgcaca
     2521 cacacggcca cacatgaggc gattccccgc gcctccagga cctcttcggg gttattgctc
     2581 accacaactt ggctctgctc caccagcact cgcctggcca tcgggcacga cctccggcgc
     2641 aacagggacg ccgtaggcca ggcgtccggt cccatcattc catctaccca gtgtgccttg
     2701 agtgccgatg ccaagcggcc tgagccgggc cgtgtcaagg ttcaaacgcg gtatggtcac
     2761 aaccacacta aggcatcggt cgccctcgga cgacctgcgc ctatgcggaa tgccgccgac
     2821 accatcgaag ccgctcgctc ggcccggggg cggtccgacg cgtgcacctc ggccaccaca
     2881 tcgcccgcac ggacttcttc gccgcgtcgg catcgcaaca agacgcctgc ggccggatcc
     2941 acggcctggc tcctgcgctc acggcccgca cccgccgcca cgcaggccag acccacctgg
     3001 cgagcgtcca cctggtgcac atagcccgac gcctgagccc ggacggggat ccgccatggc
     3061 gctgcgggga gccgatcggg ctcctcgaca cagcccgggt ctccgccctg cgcctcgacc
     3121 atgcggacca gtctctccag cgcctggccc gatgccagcg cgcgctcggc cgcctttcgc
     3181 ccctcggcgg cctcggacgc cacgccggcc agcatgagca tgcgagtgct cagctccagg
     3241 cacagctggg tcacatcggc cggcccctcg ttcttcaggc agcgcacagc ctcggccacc
     3301 tccagggcat tgccggccgc gcatccgagc ggatcgtcca tcggcgagag tgcgaccgcc
     3361 atcgaccggc cggcatcggt gccgatgcgg gccatgagtt cacccagggc gcgcgcttca
     3421 gcggcgctcg ccatgaacgc acccacaccg tacttgacgt cgatgacgat gccgtgagcg
     3481 cccgcggcca gtttcttgct catcacactt gaggccacga gcggggccga gtccaccgtc
     3541 gccgtcacgt cgcggatggc gtacagcacc cggtctgccg gtaccagctc ttcggtctgg
     3601 cccgagatca ccaggcccac acgggacgcc acatcgagca tctcctcgac actcaagtcc
     3661 acgcgcatcc cgtggatcgc ctcgagcttg tctaccgtgc ctcccgtgtg gcccagtccg
     3721 cggcccgcca tcttgacgac cgtcccgccc gccgcagcta ccagcggacc cacgaccagc
     3781 gacgtcttgt cccccacgcc tcccgtgctg tgcttgtcga cgcagaaccc gagtggcgag
     3841 aggtcaagca ttcgccccga tcgagccatg gccaacgtca gcccagtgac ctcttcctgc
     3901 gacatgccct ggaaccatac ggccatcagg aatgccgccg cctgtgcctc ggacatgcgg
     3961 cccgtgacca cgccgtccac cagagccgcg atctcgccct ccgacagcga ccggccccgc
     4021 ttcttggcct cgataagttc cacgggagtc gtcatgcgat cgaaccgtcc gtctcggcca
     4081 gcaggctcgt gccgggcccg atccgctcca cgccgagcca ttcggcggcc gtagcgccga
     4141 catcggccag cgtcgggcgc gtgcccaggt cccgccccac tacgcagccg ggctcgcgca
     4201 cgagtaccgg cacgtactcc cg
//
LOCUS       DAOSPL010000184         4220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21076_length_4220_cov_3.118848, whole genome shotgun sequence.
ACCESSION   DAOSPL010000184 DAOSPL010000000
VERSION     DAOSPL010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21076_length_4220_cov_3.118848"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            530..2248
                     /gene="recN"
                     /locus_tag="PLQ54_04715"
     CDS             530..2248
                     /gene="recN"
                     /locus_tag="PLQ54_04715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOS92590.1"
                     /translation="MLVRLWIEDFAIVDRLDVTFGGGLNVLTGETGAGKSILLDALNT
                     VLGERADVALVRSGAPGLRVCAEFEAPSSPEVREAAHELGADTTESLILDRSISAAGR
                     STCRVNGRPANVSMVRRLGEALVDLHGQHEHQSLLRVATHLEYLDAYGADAIGSLRAG
                     FAARYAEWTALRDQRERLLVSERERRQRADLLRFQVQEIESAQVSADEETELLARRDR
                     LAHAERLQQAATAAHAALTGDEATPGAEADLGRAAAQLRAVCAHDPSLQEWAVALEQA
                     LIQVEEAARAIADYRDRLEFDPEGLDACEARLRRLADLRRKYGDSLADVIAFGESAAE
                     ELRQIENADEARGEIESRIEELRERMAADAAELSAARECAARRLEAAVVANLNALGMA
                     HADFRVAVEQVGDPEGLPGATGPLAFGPTGTDRVQFLISNTPGEPPRPLARIASGGEI
                     SRTMLALKCAAACVDQVPVVVFDEVDAGLGGRAAQAVGEHLLRLAERCQVICVTHSPL
                     IAAVAHTHLSIEKTETEGRASVRVRVLSPAGRVSELERMLGGTGDRAAAEHARELAKR
                     GAALRR"
     gene            2339..3202
                     /locus_tag="PLQ54_04720"
     CDS             2339..3202
                     /locus_tag="PLQ54_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92591.1"
                     /translation="MDTYAPIAGTILIGFVCGVLGLVWTLRVLRDWRVTCRRPDGRRV
                     VTGAGLGLAVSAVLAALVAQILQFDPLIGGASWVCVLTVAIMAVAGLLADSAHPAVRP
                     GVVAPLRGRGRGTGTTGALKSLGALAAALLGARLLAPASTTELVVNAALILVWAHLLD
                     SVATGPGRALKVYWVCWFALLLFVSPAASLRTIPLAIASMLIGPYDLSGRARVGSAGA
                     LALGASLGLSACTGIASLGARVLLVAGGLCVSVYLAWRPLRAVTREHPALAVIDAWGT
                     PSAATPEAEDE"
     gene            3209..3958
                     /locus_tag="PLQ54_04725"
     CDS             3209..3958
                     /locus_tag="PLQ54_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92592.1"
                     /translation="MSGDLLAQRFSGRLTVLCGPFGVGKSEIAISLSECLASTGRDVF
                     LVDLDVVTPYFRPRDVSERLAESGVRLVSPPGEAAFLDLPAVPGGVCEVLSRGARGEA
                     SVVVDLGGRADGAQVFASVRDALTQGPHWDMLFVLNPYRPDSATAEVAGSVLRGVEEA
                     SRARVTGLVANPHLKQATNADDLVRGFARVEGAARALDLPVRLRCAPAGMAALVETGP
                     VLPLRLALLLPFESAPAGAPGRVWPGMQGAP"
     gene            3962..4171
                     /locus_tag="PLQ54_04730"
     CDS             3962..4171
                     /locus_tag="PLQ54_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOS92593.1"
                     /translation="MRGIDVRPDRCRACGLCIAVCPRQAIEYDDTLNTDGYHPIRVRD
                     AGLCSGCALCALMCPHVAITKVYRP"
BASE COUNT          577 a         1353 c         1554 g          736 t
ORIGIN      
        1 gcacgcagca gcgcccctgc aggggtgacg ggcgggtatc ggtgccggca ggcgaggatg
       61 cactgaggtg ccgccgaaac cgagcggcct cgccctgccg gtctcccgtg ccgatggcag
      121 ccacagtggc cccgctggcg ggatgcatgc gccggaatca gtgcgcttca cttcgtgaga
      181 acgggaccaa cggcgaccct ggagagcgaa gcacggtaac ggctacggga gatggtgcac
      241 gtaggtatca gtgtgtgtca gtgcctgtcc tttcgcgcat ggattctctt ataccatgga
      301 acagccccgc tcgtcgatcc cccgtctggc ccgtccgaag tcatcccctc tggccccctg
      361 gcccaatggg gagggcccgc gaagcgggaa gagtgtgctt cagcctgcac ccgagaacga
      421 agtcgccctg ccggcgcgct gcgcaggacg cccgtcgcgc gggtccgaac agactgaggc
      481 accggggggt cgcgggaccc cctggatcgg caaacgggag caaccacgca tgctggtgcg
      541 gctctggatt gaggacttcg cgatcgtcga tcggctggac gtcaccttcg gcggcggact
      601 caacgtgctg acgggggaga ccggggccgg caagtccatt ctgctcgacg ccctgaacac
      661 cgtcctgggc gagcgcgccg atgtggcgct cgtccggtcg ggcgccccgg gactgcgtgt
      721 ctgtgcggag ttcgaggccc ccagcagccc cgaggttcgc gaagcggcgc acgagcttgg
      781 ggccgatacc acggaatcgt tgatcctgga ccgttcgatc tcggcagccg gccgcagcac
      841 ttgccgcgtg aacgggcgcc ccgcaaacgt gtcgatggtg cgccgtctgg gggaagccct
      901 cgtggacctc catggccaac acgagcatca gtcgctgctg cgtgttgcta cgcatctcga
      961 gtaccttgac gcctacgggg ccgatgcgat cggcagcctc cgtgcgggat tcgccgcgcg
     1021 ttacgccgag tggaccgcgc tccgcgatca gcgcgagcgg ttgctcgtga gcgaacgcga
     1081 gcggcgtcag cgagccgacc tgctgcggtt ccaggtgcag gagatcgaga gcgctcaggt
     1141 ctcggccgac gaggagacgg agctcctggc tcggcgcgac cgactggccc atgccgagag
     1201 gcttcagcag gcggcgaccg cggctcacgc tgccctcacg ggagatgagg ccacgccggg
     1261 cgcggaggcg gacctcggaa gggccgcggc ccagcttcgc gccgtgtgtg cccatgaccc
     1321 gagcctccag gagtgggcgg tggccctcga gcaggctttg atccaggtcg aagaagcggc
     1381 tcgcgcgatc gccgactacc gggaccggct ggagtttgac ccggagggcc tggatgcatg
     1441 tgaagcccgt cttcgccgac tggcagatct ccggcggaag tacggtgact cgctagctga
     1501 tgtcatcgcc ttcggcgaga gcgctgcgga ggagctgcgc cagatcgaga acgccgatga
     1561 ggcacggggt gagatcgagt ctcgcatcga ggaactccgt gagcgcatgg cggccgacgc
     1621 ggctgagctc tcggcggcga gggagtgcgc ggcgcggcgc ctcgaagcag cggtcgttgc
     1681 gaatctgaat gctctcggga tggcccatgc ggatttccgg gtggccgtcg agcaggtagg
     1741 tgatcccgag gggctgcccg gcgcgacagg gcctctcgcg ttcgggccca cgggaactga
     1801 tcgcgtgcag ttcctgatca gcaacacgcc cggcgaacct ccgcgcccct tggctcggat
     1861 tgcctctggg ggtgagatct cgcggaccat gctggcgctc aagtgcgccg ccgcctgcgt
     1921 cgaccaggtc cccgtggtgg tcttcgacga ggtggacgcc ggcctcggcg ggcgagcggc
     1981 tcaggcggtg ggtgagcacc tgctccgtct ggccgagcgc tgccaggtca tctgtgtgac
     2041 ccactcgccc ctgattgccg cggttgcgca cactcacctg tccatcgaga agaccgagac
     2101 ggagggacga gcttcggtgc gtgtgcgggt cttgagccct gccggccgcg tgtcggagct
     2161 cgagcgcatg cttggaggaa ctggcgaccg agcggcggcg gagcacgccc gcgagctggc
     2221 caagcgcggc gcggcactcc ggcgttgagc gggcgggaag gattgcggca accaagggtg
     2281 aagcactcac gggtgagttc catggctcgg gttgtgggag ggcagttgcc gaccgcatat
     2341 ggatacgtat gcacccatcg cgggaacgat tctcatcgga ttcgtctgcg gcgttctggg
     2401 cctcgtgtgg acgctgcggg tcctgcgtga ctggcgtgtg acgtgccgcc ggccggacgg
     2461 tcgccgagtg gtgacgggtg ccggcctggg tctggcggtt tccgccgtcc tcgccgcgct
     2521 cgtcgcgcag atcctccagt tcgatccgct catcggcggg gcgtcttggg tctgcgtcct
     2581 gacggtggca atcatggccg tggcaggtct cctggccgac tcggcccatc cggccgtgcg
     2641 cccgggtgtg gtcgctcctc tgcgcggcag aggtagaggc acgggcacca cgggggccct
     2701 caagagcctg ggcgccctgg cggccgctct cctcggtgcg cggctgctgg cacccgcctc
     2761 gaccaccgag ctggtggtga atgccgcgct gatcttggtg tgggcacact tgctggattc
     2821 ggtcgccacg ggtccaggcc gggcgctcaa ggtgtactgg gtgtgctggt ttgccttgct
     2881 gctgtttgtg agcccggcgg ccagcctccg cacgatcccg ctggccattg cctcgatgct
     2941 catcggtccc tacgatctgt ccgggcgggc acgcgtcggg tcagccggtg ccctggccct
     3001 tggcgcgtcg ttggggctct cggcatgcac gggcatcgcc tcgctggggg cacgagtgct
     3061 gctcgtggcg ggcgggctct gcgtctcggt ctatctggcg tggcgtcctc tgcgcgcggt
     3121 cacccgagag catccggcgc tggcggtcat cgatgcctgg ggcacgccct cggctgccac
     3181 accggaagcc gaagacgagt gagccggggt gagtggcgat cttctggccc aacggttctc
     3241 ggggcggctt acggttctct gcggcccgtt cggggtgggc aagagcgaga ttgccatcag
     3301 tctctccgag tgcctggcgt ccaccggccg cgatgttttc ctggtggatc ttgacgtggt
     3361 gaccccctac ttccggcctc gtgatgtcag cgagaggctg gccgagagtg gtgtacggct
     3421 cgtgagtccg ccgggtgagg cggcattcct cgacctcccc gcggtgcccg gtggggtctg
     3481 tgaggtgctg tcgcgcggtg cgcgaggtga ggcgtcggtt gtggtggatc tgggtgggcg
     3541 agccgatggc gctcaggtct tcgcctccgt acgcgacgcc ttgacgcaag gaccccattg
     3601 ggacatgctg ttcgtgctca atccgtaccg ccccgacagc gccacggcgg aggtcgcggg
     3661 ctcggtgctc aggggagtgg aggaggcgtc gcgagcccga gtgacgggcc tggtggccaa
     3721 tccccacctg aagcaggcga cgaacgcgga tgacctggtg aggggcttcg cacgagttga
     3781 gggtgccgcc cgagccctgg acctgcccgt ccgtctgcga tgcgcgcccg ccggcatggc
     3841 ggcactggtc gagaccggtc ccgtcctgcc gctccgtttg gctttgctgt tgccgttcga
     3901 atcggctccc gccggcgcgc cgggccgtgt gtggccgggg atgcaaggag cgccatagcc
     3961 catgaggggc atcgatgttc ggccggatcg ttgccgtgcg tgtggcttgt gcatcgcggt
     4021 atgcccgcgc caggccatcg agtacgacga cacgctcaac accgacggct accaccccat
     4081 tcgggttcgt gatgcgggcc tgtgctccgg ctgcgcgctt tgcgcgctta tgtgccccca
     4141 cgtggccatc acaaaggtgt atcgtccgtg aggttcgtgc acgaggggga tccccgctat
     4201 ggggagcccg agctgctcaa
//
LOCUS       DAOSPL010000185         4215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21104_length_4215_cov_3.362019, whole genome shotgun sequence.
ACCESSION   DAOSPL010000185 DAOSPL010000000
VERSION     DAOSPL010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21104_length_4215_cov_3.362019"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PLQ54_04735"
     CDS             complement(<1..775)
                     /locus_tag="PLQ54_04735"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOS92594.1"
                     /translation="MDAVAAELEGFDHGRLARQLTSLTHNSEDFGFEGRARLKVRHGN
                     KDGAAFANVMMAEPPIDYSTLGEARLVRAHAYFAAQAREWIQAGGEGSVPTRAEALAK
                     ALMQGIQLVVITLSPQENSQEIFETLNARGTPLTAGDLIKNLIFQRLALEGVDTGKAY
                     QDHWRFFENPFWEKEISLGRYAVERVSLFLNHWLVAQVGEEVSTRSTYTRFKRWAEHE
                     TGQSMADILSQLHGAATAYQEWIEGASKRTGALSRQELFV"
     gene            complement(793..1056)
                     /locus_tag="PLQ54_04740"
     CDS             complement(793..1056)
                     /locus_tag="PLQ54_04740"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOS92595.1"
                     /translation="METQVKTPRQVFNLPQYLDVPLFQRPYVWDEELQWSPLWEDIRR
                     VTELRMASAPEATHFLGAIVMQEVSGSGMGDAETYSSSMANSA"
     gene            1237..1740
                     /locus_tag="PLQ54_04745"
     CDS             1237..1740
                     /locus_tag="PLQ54_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92596.1"
                     /translation="MGVMDRLAERWANRESGLEPIMHSQSILVRATAADVYAFLIDPE
                     SMRVIVADAANAFRVPGTPTGEIGEQVCVVIHHDTGHRVDLWETTDLIPEREIRQRLV
                     SVDTPWEEHLTIEEYGPAAARLTDTHEGFVSLGRGAEVRAYLDSSVNASLDRIRVALE
                     ERSKGQR"
     gene            1744..1944
                     /locus_tag="PLQ54_04750"
     CDS             1744..1944
                     /locus_tag="PLQ54_04750"
                     /inference="COORDINATES: protein motif:HMM:NF027879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MIT C-terminal domain-containing protein"
                     /protein_id="HOS92597.1"
                     /translation="MSSSILTSLEGFGTRSDTGWKNLLGRGLDIFHKGSESQCDKGAR
                     GEDFRQLVAFRVTYIHKREVSE"
     gene            2054..3637
                     /locus_tag="PLQ54_04755"
     CDS             2054..3637
                     /locus_tag="PLQ54_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92598.1"
                     /translation="MTDADALRWFWIGRLAWDVTEWAGPAWLFAEEMPTPAGVDFPIA
                     SALAISRIARRIDDGDTYRQILDDLVRAQVTDEWFGNARQQAEAVAEAISAGTWPGKV
                     PLDARGAELFFTEAWAAARSLQVGPARVQVELVQRWGLGDSRMLDYLCSLAPTPFDGH
                     VDLLTRIVEMPDQDEMKAILTNLRWQASQDPNHKAAVEGFGGLPGHIAAVAEEMAYAI
                     RLDRKLERLCQASLEQAPDRHAGFADARQVVKEQRAGLVAVMQSFTAYEVDIVREALD
                     QDASASVRTTSKVSLDTVAHLPTTNPNQALPAPVRTKASAVKRDLDPIAISVRLRRLW
                     TDEIGPWLEEIDNDLGSVWRETLLRRVGLTLDVVSPINFGDGDPMIDIREDFIARLAD
                     ATNGQVNRVRVAFLGHAKYTPPRRAKTTDLDIFDPGDTAALTASFGSDIGGRISRNLG
                     DEAAAVLSELRVAGVSRHRVWFVAPTPDAVSAVAKTPGAAHALWDAVRRTQGVAKDPL
                     YCIYSDKPVWSDSRGINLG"
     gene            3756..4181
                     /locus_tag="PLQ54_04760"
     CDS             3756..4181
                     /locus_tag="PLQ54_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92599.1"
                     /translation="MIIKSVSELKGKELLPGSIEISPGVRIQPDGLDADRTIFVEAYA
                     RQGKLVGAQLKKVAQDVLKFTLLKTAYPTAEFVLAFASAEAMDSVRGWLLEAARANGV
                     QMLLTQISDEDVTRLLLAQDRMRQGMKQTKADLPLDTEA"
BASE COUNT          795 a         1282 c         1350 g          788 t
ORIGIN      
        1 agacgaagag ctcctggcgc gacagagctc cggtgcgctt cgaggcgccc tcgatccatt
       61 cttggtaggc cgtggccgcg ccgtgaagtt gggacagtat gtcggccatc gactgccccg
      121 tctcgtgttc ggcccaccgc ttgaatcgcg tgtaggtgct ccgagtgctg acctcctctc
      181 cgacctgggc caccaaccaa tggttgagga agagcgaaac ccgctccacg gcataccgac
      241 cgagactgat ttccttctcc caaaaggggt tttcgaagaa tcgccaatgg tcctgatagg
      301 ccttgcctgt gtcgacgccc tcgagagcaa gacgttggaa gatgaggttc ttgatcaagt
      361 caccggcggt gaggggagtg ccgcgggcgt tcagggtctc gaagatctcc tgtgagttct
      421 cctgcggaga cagggtgatg acgacgagct ggatgccctg catcagtgcc ttggccagtg
      481 cctctgcacg agtaggaacg ctgccctcac ctcccgcttg gatccactcc ctggcctggg
      541 ccgcgaaata ggcgtgggcg cggacaagcc gagcctcacc caacgtcgag tagtcgatag
      601 gcggctcggc catcatgacg ttcgcgaagg ccgccccgtc cttgttgcca tgccgcacct
      661 tcaaccgggc ccgaccttcg aagccgaagt cctccgagtt gtgggtcagg ctggtcagtt
      721 ggcgggccag gcgtccgtgg tcaaagccct ccagctctgc agccaccgca tccatgaaga
      781 gttgcagggt tgtcaggcgc tgttggccat cgatgaggag taggtctcgg catcccccat
      841 ccctgacccg gagacctcct gcatgacgat ggcgcccagg aagtgagtgg cctccggtgc
      901 cgatgccatg cggagttctg tgacgcgacg gatgtcctcc cagagcggcg accactggag
      961 ttcctcgtcc cagacgtagg ggcgctggaa gagtggcaca tcgaggtact gcgggaggtt
     1021 gaagacctgc cggggtgtct tgacctgcgt ttccatccga gcctcccgca attctccgag
     1081 cgcctgcggc tcgaccctag aggaccgccg agccggcatg gagcgcatga acggcatcca
     1141 ttgccagaca gctgctcgcg gcgtggggac cttcgccgag gatgccgccc attcggatga
     1201 tgcggcgccg acgtgtgagc cggtagacag cgtgtcatgg gggtgatgga ccggctggcc
     1261 gaacgctggg ccaatcgtga gagcgggctc gagcccatca tgcatagtca gtcgatcttg
     1321 gttcgtgcca cggctgcgga tgtatacgca ttccttatcg accctgagtc catgcgcgtg
     1381 atcgtggccg acgcagccaa cgcgttccgc gtcccaggga cgcccactgg cgagattggc
     1441 gagcaggtct gcgtagtcat tcatcacgac acgggtcacc gcgtcgacct ttgggagacg
     1501 acagacctca tacccgagcg cgagatccga caacggctgg tgtccgtgga caccccgtgg
     1561 gaagagcacc tcaccatcga ggagtatggc ccggcggcag cccggctcac cgacacgcac
     1621 gagggatttg tgagcctggg gaggggggct gaggtccggg cctacttgga ctcgagtgtg
     1681 aatgcgagcc tcgaccgcat ccgtgttgcg ctcgaagagc ggtcgaaggg ccagaggtga
     1741 cctatgtcgt cttcgatact gacatcgctc gaaggattcg gcacgcggtc tgacaccggt
     1801 tggaagaacc tccttggccg cggcctcgac atcttccaca agggctccga gagtcagtgc
     1861 gacaaaggcg cccgtgggga agacttccgc cagctcgtcg cattccgcgt tacatacatc
     1921 cacaaacggg aggtgtccga gtgatcccgc caaaccgaga cgcgcgcgat tcacgcttcg
     1981 tcactggctc atggagcgag acagagcagg aggcttcgga gcttcccgaa ccccaggatg
     2041 agcgggtcaa tacatgacag atgctgacgc gctgcgatgg ttctggatcg gtcgcttggc
     2101 ttgggacgtg actgagtggg ccggaccggc ctggctgttc gcagaggaaa tgccgacccc
     2161 agcgggggtc gactttccga tcgcttcagc cttggccata tctcgtattg ctcgcaggat
     2221 cgatgatggc gatacgtaca gacagattct tgatgacttg gtgcgagcac aagtcaccga
     2281 tgaatggttc ggcaacgctc ggcaacaggc agaagcagtt gcagaggcca tctcagctgg
     2341 gacttggccc gggaaagtac cgctggatgc gcgaggcgcc gaactgttct tcaccgaagc
     2401 gtgggcggcc gctcgatcac tccaagtggg gccagcgcgg gtgcaggttg agttggtcca
     2461 acgctgggga ctgggcgatt cgcggatgct cgactatctc tgctctctcg cccccactcc
     2521 gtttgatggg catgtcgatt tgctgacccg gatagtcgag atgcctgacc aagatgagat
     2581 gaaggcgatc ttgacgaacc tccgatggca ggcaagtcaa gatccgaatc acaaagcagc
     2641 cgtcgagggg ttcggtggcc tcccgggaca catcgcggct gtcgcggagg aaatggcata
     2701 cgcgatacgg ctggaccgca agcttgagcg cctgtgtcaa gcttccttgg agcaggcgcc
     2761 ggatcgccat gcgggtttcg ctgatgcccg ccaagttgtc aaggagcagc gggcaggctt
     2821 agttgctgtg atgcagtcat tcactgccta cgaggtcgac attgtccgcg aagccctcga
     2881 tcaagacgcg tcagcaagtg tcaggacaac ttcgaaggtg tcgcttgaca cagttgcaca
     2941 cctacccacg acgaatccca accaagcgct gccagcgccc gtcagaacca aagcgtccgc
     3001 ggtgaagaga gatcttgacc ccatcgccat ctcggtgcgt ctgcgccgtc tgtggaccga
     3061 tgagattggc ccgtggcttg aggagatcga caacgacctg ggctccgtct ggcgggagac
     3121 gctcctgagg cgtgtgggcc taacgctcga cgtcgtctcc cccatcaact tcggcgacgg
     3181 cgaccccatg attgacattc gcgaggactt catcgcccga ctcgccgatg ccaccaatgg
     3241 ccaggtgaac cgggtgcgcg tcgcattcct cgggcacgcg aaatacacgc cgccacgacg
     3301 agcgaagaca acggatctgg atatctttga ccccggtgac acggctgcgc tcaccgcgtc
     3361 attcggatcg gatattggcg gccggatctc ccgtaacctc ggggatgagg ctgcggcggt
     3421 cttgtccgaa cttcgggtgg ctggagttag tcgccatcgc gtctggttcg ttgcgccgac
     3481 gccagatgct gtcagcgccg tggccaagac gccgggagca gcccacgctc tttgggatgc
     3541 ggttcggcgc acgcagggcg tagcgaagga cccgctctac tgcatctact cggataagcc
     3601 cgtctggtcg gactcacggg ggatcaacct ggggtagttc cagcgagggc gccaatggtg
     3661 aggccgacgt gcgtgcatat ccacgccata ctgatggcgt gaccgctgct catgcccacg
     3721 cttcgacctc aaccgagcac tcgctcgctg aggctttgat catcaagtcc gtgtccgaac
     3781 tcaagggcaa ggagcttctg cccggcagca ttgagatttc tcccggggtg cgcatccagc
     3841 ccgatggcct ggacgccgat cgcacgatct tcgttgaggc ctacgcgcgc cagggcaaac
     3901 tcgtcggcgc gcaactcaag aaggtcgcgc aggatgtcct gaagttcacc ctcctgaaga
     3961 cggcataccc gacggccgag ttcgtcctcg ccttcgcgag cgccgaagcc atggattccg
     4021 ttcggggatg gttgctggaa gccgctcgag caaatggggt gcagatgctt ctgacgcaga
     4081 tctcggacga ggacgtgacc cgactgctgc ttgctcagga ccggatgcgc cagggcatga
     4141 agcagaccaa ggccgacctt ccgctggaca ccgaggccta atccacttcc gaacgccggt
     4201 acagggtggc gggcc
//
LOCUS       DAOSPL010000186         4212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21124_length_4212_cov_2.914842, whole genome shotgun sequence.
ACCESSION   DAOSPL010000186 DAOSPL010000000
VERSION     DAOSPL010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21124_length_4212_cov_2.914842"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1929
                     /locus_tag="PLQ54_04765"
     CDS             <1..1929
                     /locus_tag="PLQ54_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92600.1"
                     /translation="SPCFTLRPKRLSPGQSLTFDIVLKRFDKPACRYRDVLRWYLQSA
                     ESSDPLVRNLFPWDGRRRARPFPVGNLGSGLGRISDPGVPPDRLVDVFRQRRVGGLWF
                     QGWGPWDETYPTEGEWYTEYWARLSAAQIRDEIDWERRNGIAPYLYCRQFLTEQGVYD
                     DRPPLREWLGRDENGNRQAWGDWPVPEPIAKEIGFSVLQQSCADFGNPDYLAWYEGRV
                     KACLEAYRPAGIAWDMGWGAGNTWGYSAANPRTPNGDGMLRAQADLWEWLHAEHPEMR
                     AISNEAFGTPSQLFADGILIEGGFASGKTDLDYEAAKALGTTVISYEYPEQYATRLRG
                     LPTRDARYVQMRYRAIGVNTKSDRYVVYPSQEVVGREGPAIACSNLQADGEWHVATFD
                     LRGLPSIEEVRGMAFGMDSAAEEARLWVEYIRFSATADGPALPPEPGRFPSEITAADA
                     AAFDPRPGWVQNAPEEYGVRRDGDAIEFTVRGEGKGMSWSFFPARAELAQEYLHVLSL
                     GACLGAGLRQEWGAMNAFSAEAMALAPLVGSHDVEVFPASGPVTAAAWGRAGRLLLAV
                     HNPSDEQQAVTVEVSRRAVRQAAADNVRGGLSRVMAAGAEQSRPGPGPHTTADGSLRF
                     RVRLGPGEALLWGNWQWE"
     gene            complement(1953..2183)
                     /locus_tag="PLQ54_04770"
     CDS             complement(1953..2183)
                     /locus_tag="PLQ54_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92601.1"
                     /translation="MADLTRLNQATSDLIMDIVGDLGTCTAEQVQAELLRRDGTAAEL
                     DELVRYMEFLRSGFPRKLAHAGPGRWVLVVLA"
     gene            complement(2192..3346)
                     /locus_tag="PLQ54_04775"
     CDS             complement(2192..3346)
                     /locus_tag="PLQ54_04775"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOS92602.1"
                     /translation="MGVDRFDGKELEYLGRILSSGSLAGRGDGFVGELERAFAEAYGV
                     QHAIAANAAMSLLISSTFAAGAGAGDEVLCDPIVQFHAIGALWQNAIPTWADVCADTW
                     LMDPESARANITPQTKAIFVTNLWGQPAELDALRKVADERKVVLIEDCAHAMFLPYKG
                     RYAGTWGHVGIFSFCQGKHMTTGDGGMAITDDDELAARIRSVTCFGESPPHLATVFRM
                     TEMQAAIGLAQLERVKGYIEEYQRAYAQLSAALEGCAWLQPRAIREGCGNSPYIFSFL
                     YRGDEHGIDLDAFKQALFATGDYWNVGFTQVPAYKYKLFTHPLAYQDKGCPRHQCPYY
                     EGSYEYRDGLCPTAEAVLPRLVNTNCMVSEETAKRVAEGLRRAIETTEKG"
     gene            complement(3377..4207)
                     /locus_tag="PLQ54_04780"
     CDS             complement(3377..4207)
                     /locus_tag="PLQ54_04780"
                     /inference="COORDINATES: protein motif:HMM:NF021601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HOS92603.1"
                     /translation="MSLFEHTRRFLDAEGVPASFMVVPRGEGGWQLDRQPEWLEALRS
                     AERDGHDCQLHGLDHANCEFGPIPDLIRTLDGTDAEAGFRSDSEQFGHMWRPEAYYEK
                     LQTAMAIFERAFERRPLVFRTGALSQTPELYDVMADAGMRYASNLITDPRGWAYIVER
                     YDDPGDWDPAVPPGPHRLTDQIINLPIISEYAWYLTEAKIPRHLALAIDDLERVFAAG
                     GVFVLVCHVQCVGAEDGLSRKLLHQLLQVARQDFGVEFGTLAALVQGIERGDVSLFTP
                     "
BASE COUNT          681 a         1400 c         1468 g          663 t
ORIGIN      
        1 agcccctgct tcaccttgcg gcccaagcgg ctctcacccg ggcagtcact caccttcgac
       61 attgtgctga agcggttcga caagcccgcc tgccggtacc gcgatgtgct ccgctggtat
      121 ctgcagtcgg ccgagagctc cgatccgctg gtgcgcaacc tgttcccctg ggacggtcgc
      181 aggcgggccc ggccgttccc ggtgggcaat ctgggaagcg ggctgggtcg catctcagat
      241 ccgggcgtcc cacccgatcg cctggtggac gtattccgcc aacgccgcgt cggcgggctc
      301 tggttccagg gctgggggcc ctgggatgag acctatccga ccgagggcga gtggtacacc
      361 gagtactggg cccggctctc ggcggcccag atacgcgatg agatcgactg ggagcggcgg
      421 aatggcatcg ccccatacct ctactgccgg cagttcctga ccgagcaggg cgtgtacgac
      481 gaccggccgc cgcttcgcga atggctgggt cgcgatgaga acgggaaccg ccaagcgtgg
      541 ggcgactggc cggtcccgga gcccattgcc aaggagatcg gcttctccgt tctgcagcag
      601 tcctgcgccg acttcggcaa ccccgattac ctcgcgtggt acgagggtcg agtgaaggcg
      661 tgcctggagg cgtaccggcc cgccggcatc gcctgggaca tgggctgggg ggcgggcaac
      721 acttggggct attcggccgc caacccgcgg acgcccaacg gtgacggcat gctccgcgcc
      781 caggccgacc tgtgggagtg gctccacgcc gagcatcccg agatgcgcgc catctcgaac
      841 gaggccttcg gcacaccctc gcagctgttc gcggacggca tcctgatcga gggcggcttt
      901 gcctcgggca agaccgatct cgactacgag gccgccaagg ccctcggcac gaccgtcatc
      961 agctacgagt acccggagca gtatgcgacc cgcctcagag gcctccccac ccgcgacgca
     1021 cggtacgtgc agatgcgcta tcgcgcgatc ggcgtcaaca cgaagagcga ccggtacgtg
     1081 gtctatccct cgcaggaggt cgtgggcaga gaagggccgg ccatcgcctg cagcaacctc
     1141 caagccgatg gtgagtggca cgtggccacc ttcgacctgc gcggcttgcc gagcatcgag
     1201 gaggtcaggg gaatggcctt cgggatggac tcggccgccg aggaggcccg gctctgggtg
     1261 gagtacatcc gcttcagcgc cacagccgac gggccagcac tgccccctga accgggacgc
     1321 ttcccatcgg agatcacggc ggccgacgcg gcagcctttg acccgcggcc gggatgggtg
     1381 cagaacgccc cggaggagta cggcgtccga cgcgacggcg acgccatcga gttcaccgtc
     1441 cgcggggagg ggaagggcat gagctggtcc ttcttcccgg cgcgggctga gctggctcag
     1501 gagtacctcc acgtactgag cctgggcgcg tgcctcgggg cgggcttgcg ccaggagtgg
     1561 ggggccatga acgcgttctc ggccgaggcc atggccctcg cgccgctggt ggggagccac
     1621 gacgtggagg tcttcccggc atcgggcccg gtcacggccg cagcctgggg gcgagccgga
     1681 cggctgctct tggcggtcca caaccccagc gacgagcaac aggccgtgac cgtcgaggtg
     1741 tcccgtcggg cggtgcgtca ggctgcggcg gacaacgttc gcggcggctt gagtcgcgtc
     1801 atggcagccg gtgccgagca gtcgcgcccc ggccccgggc cgcacaccac ggcggatgga
     1861 tcactccggt tccgcgtgcg cctgggcccc ggcgaggcgc tgctgtgggg caactggcaa
     1921 tgggagtgag gcgaccagcg aagggctggg gcctacgcga gcacgaccag cacccagcgc
     1981 cccgggcccg cgtgggcgag tttgcgcggg aagccggacc gcaggaactc catgtagcgg
     2041 acgagttcgt cgagctccgc cgccgtgcca tcccgccgca acagctccgc ctgaacctgc
     2101 tccgccgtac acgttcccag gtcgccgacg atgtccatga tgaggtccga ggtagcctgg
     2161 ttgaggcgcg tgagatcggc catggcccct gctatccctt ctccgtcgtc tcgatggccc
     2221 ggcgaagccc ttcggcgact cgcttggcgg tctcctcgga gaccatgcag ttggtgttga
     2281 ccaggcgcgg cagaaccgcc tcggccgtgg ggcagaggcc gtcgcggtac tcatagctcc
     2341 cctcgtagta ggggcactgg tgccgcgggc agcccttgtc ctggtaggcg agcggatggg
     2401 tgaagagctt gtacttgtag gcaggcacct gggtgaagcc cacgttccag tagtccccgg
     2461 tggcgaacag cgcttgcttg aaggcatcca ggtcgatgcc gtgctcatcg ccgcgataga
     2521 ggaatgagaa gatgtacggt gagttcccac acccctcgcg gatggcgcgc ggctggagcc
     2581 aggcacagcc ctcgagtgcc gccgagagct gggcataggc ccgctggtac tcctcgatgt
     2641 agcctttcac gcgctccagc tgcgccaggc cgatggcggc ctgcatctcg gtcatgcgga
     2701 acacggtggc caggtgcggc ggcgactcac cgaagcaggt gacgctgcga atgcgggccg
     2761 ccagttcgtc atcgtccgtg atcgccatgc cgccatcgcc ggtggtcatg tgcttgccct
     2821 ggcagaaact gaagatgccc acatgacccc aggttcccgc ataccggccc ttgtagggga
     2881 gaaacatggc gtgggcacag tcctcgatga gcaccacctt gcgctcatcg gccacttttc
     2941 tcaatgcgtc cagttcggcc ggctgccccc agaggttggt gacgaagatc gccttggtct
     3001 ggggggtgat gttggcccgc gcggactcgg gatccatgag ccacgtatcg gcacagacgt
     3061 cggcccaggt cgggatggcg ttctgccaga gggcaccgat ggcgtggaac tggacgatgg
     3121 ggtcgcacag cacctcgtcg ccggccccgg ctccggcggc gaaggtcgac gagatcagca
     3181 gggacatcgc cgcgttggcg gcaatggcgt gctggacgcc ataggcctcg gcgaatgccc
     3241 tctccagctc gcccacgaag ccgtcgccac gcccggccag cgaacccgaa ctgaggatcc
     3301 gtccgagata ctccagttcc ttaccatcga atcggtccac acccatgggg gtgcctcctt
     3361 gcgtgggtcc atgtggtcag ggagtgaaca acgacacgtc gccccgctcg atgccctgca
     3421 ccaatgccgc cagggttccg aactcgacac cgaaatcctg gcgcgcgacc tgcaggagct
     3481 ggtgcagcag tttccgcgac aacccatcct ccgccccgac gcactgcacg tgacacacga
     3541 gcacaaacac accgcctgcg gcgaagacgc gctccaggtc atcgatggcc agcgccaggt
     3601 gccgggggat cttggcctcg gtcagatacc aagcgtactc gctgatgatc ggtaggttga
     3661 tgatctgatc ggtgagccgg tggggcccgg gcggtaccgc gggatcccag tcgcccgggt
     3721 catcgtagcg ctcgacgatg taggcccagc cccgcgggtc ggtgatgagg ttcgacgcgt
     3781 agcgcatacc ggcgtcggcc atcacatcgt agagctcggg cgtctgcgac aaggcgccgg
     3841 tgcgaaagac caacggccgg cgctcgaagg cgcgttcgaa gatggccatg gcggtctgga
     3901 gcttctcgta gtaggcctcg ggccgccaca tgtggccgaa ctgctccgag tccgagcgga
     3961 agccggcctc ggcgtcggta ccgtccaggg tgcggatcag gtcggggatg gggccgaact
     4021 cgcagttggc gtggtcgagg ccgtggagct ggcagtcgtg cccgtcgcgc tcggcggagc
     4081 ggagcgcctc gagccactcc ggctgacgat cgagctgcca gccgccctca ccgcgcggca
     4141 cgaccatgaa gctggccggt acgccctcgg catcgaggaa ccggcgagtg tgctcgaaca
     4201 gggacaggtc ct
//
LOCUS       DAOSPL010000187         4209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21150_length_4209_cov_3.098941, whole genome shotgun sequence.
ACCESSION   DAOSPL010000187 DAOSPL010000000
VERSION     DAOSPL010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21150_length_4209_cov_3.098941"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="PLQ54_04785"
     CDS             complement(<1..1201)
                     /locus_tag="PLQ54_04785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS92604.1"
                     /translation="MGRAAFLYLSSSPYEATGGGQRPAQLARALARQGYDVAFYHGED
                     ACRLDGRVQILGPPELDPWCAHRRGIAISALPEHLHLSRRLAELGWGIVYESLDDWTA
                     FAAGGDVSADMLEGEAELMRLARLVTCTAPRLEERARALGAARTALIRNGGPGLFTHS
                     TASWAPGDPVRVCFVGGLEGSWLDWTALGYVAAQEDCRLTIVGELHAQVPRWPGVTYT
                     GAMGNAAAIDLMARHHVGLVPFRGPIAPSVDPIKAYEYWAAGCWVVTSEAVEPLRGRP
                     YTRSYEQPYGIGAAIHAAVQSRRRQPVDPDFVAGSSWDRRASELLAALCLAGLLPEPA
                     EASTPPRLDANESACPVCGRSSANAAMPPIGPYEIPLEFGDVIWDEPIGRSPTLDRPP
                     RADGTHAR"
     gene            1390..2298
                     /locus_tag="PLQ54_04790"
     CDS             1390..2298
                     /locus_tag="PLQ54_04790"
                     /inference="COORDINATES: protein motif:HMM:NF016349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin"
                     /protein_id="HOS92605.1"
                     /translation="MSDQTRGTGGPGRGPGTEDLTRPYNLTRQYMLAEGAEVTPTDAV
                     QATVNSLVERLLVGRRLFEVVHSGTDVPSGIVWAQTQAHGGDKAEGPTVEVSTAQIAA
                     PSTRLWAAVEIDESEIDLRACIARSSALVDVARQMALQEDRVVFKQVWERSSRLPATH
                     GGRSADGDPAATALTTALGTLRGAGHRGGVALLLAAGDGFRDIESELARTGCIARVAP
                     LFALGIHAVPLMASGDALVVAPEGSGASITVGQNYAVSCIECSGPRLRFGICTTFGVS
                     PGAPAAQCRLSVPEPPAPGGAQRKRG"
     gene            2386..3669
                     /locus_tag="PLQ54_04795"
     CDS             2386..3669
                     /locus_tag="PLQ54_04795"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOS92606.1"
                     /translation="MARHRLLLRCLLGLAVLCSVVSSVSAEATLRVNTDRPDAIYACG
                     DDATFAVGLQRDGGPVVEATVQYRLTLDGGKLLGEGELAVRDGRAEVRATLAEPGFVR
                     LSVTFAGEDGHIDAMAAAAFDPEHIVPSADEPADFVEFWARQKAELATIAPDPRLEER
                     ADLSSDQRTVYKISLANIGGSRVYGWLAVPKSPGPYPAVLTVPWAGVYPTPVGLIDWA
                     DRGFLAMAISAHDYDVDMPAEEYKKLEEGPLKGYPHQGRESRETGYFRRVFLGCVRAV
                     DYLTSREDWDGTSLIVTGSSQGGGLSLVTAGLDRRVTALAANVPALCDHLGYKAGHQP
                     GWPRLVTEGAADQEQAAGYFDAVNFARHVACPAIVGVGFIDTVCPPTGVYAAYNVLGG
                     PKQIVPTPLMGHSQSEVYGALLHRWIPEQAGRAAR"
     gene            4027..>4209
                     /locus_tag="PLQ54_04800"
     CDS             4027..>4209
                     /locus_tag="PLQ54_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92607.1"
                     /translation="MDLRAILDRSDIPEDVRRALQAHLADPSTREDRLYRELFERSSD
                     AVFLLHGTRFIDCNLAA"
BASE COUNT          634 a         1431 c         1492 g          652 t
ORIGIN      
        1 cgcgggcatg ggtgccgtct gcgcgcggcg gccggtccag cgtgggcgaa cgtccgatcg
       61 gctcgtccca gatcacgtcg ccaaactcga gcgggatctc gtaaggcccg atgggcggca
      121 tggcggcatt cgcgctggag cgtccgcaca cggggcaggc ggactcgttg gcgtcgagtc
      181 ggggcggcgt cgacgcctct gccggctccg gcagcagtcc ggccaggcag agggcggcga
      241 gcaactcgct ggctcggcgg tcccaggacg accccgcgac gaagtcgggg tcgaccggct
      301 gcctcctgcg cgactggacg gcggcatgga tggccgcccc gatcccgtaa ggctgctcgt
      361 aggagcgcgt gtacgggcgg ccacggagtg gctccaccgc ttcgctggtg accacccagc
      421 agcctgcggc ccagtactcg taggccttga tgggatccac ggacggcgca atgggtccgc
      481 ggaacggcac caagcccacg tggtgccggg ccatgaggtc gatggcggcc gcgttgccca
      541 ttgcgcccgt gtatgtgaca ccgggccacc ggggcacttg cgcgtgcagt tcgccgacga
      601 tggtgagtcg gcagtcctcc tgagcggcga cgtacccgag ggcggtccaa tcgagccagg
      661 agccctcgag gccaccgaca aagcatacgc ggacggggtc gccgggagcc caactggcgg
      721 tggagtgcgt gaagagtccc gggccaccat tgcggatgag cgccgtccgg gcggcgccca
      781 gggcccgggc gcgctcctcg agccgcggcg ccgtgcaggt cacgagccga gccagccgca
      841 tgagctcggc ttcgccctcg agcatgtcag cggagacgtc ccctcccgcc gcgaaagccg
      901 tccagtcgtc cagcgattcg tagacaatac cccacccgag ctccgccagg cggcgggaca
      961 ggtggaggtg ttcggggagc gcgctgatgg cgatcccccg ccgatgggcg caccacgggt
     1021 ccagctcggg cggtcccagg atctgcacgc ggccatcaag ccggcaagcg tcctcgccgt
     1081 ggtagaaggc gacgtcgtac ccttgccgcg ccagcgctcg ggcgagctga gccggccgct
     1141 ggccgccgcc ggtggcttca tagggtgaac tggatagata gagaaacgcc gccctgccca
     1201 tggtgcatca cccagcactt cacaatggct ttgctaagga cagtcgtacc ggtggtggct
     1261 tccacaatga gctagcaaat acctgcaagg gtttgaaggt gatatgcaca gagttcagga
     1321 aacgggccag gggagtgata atagcattac cgctggcatt gcgagcggac ccatgcggag
     1381 gtggcactca tgtccgacca gacgcgtggt acgggtggtc cgggccgcgg cccgggtacc
     1441 gaggatctca cgaggccgta caacctgact cggcagtaca tgctggccga gggcgccgag
     1501 gtgaccccca cagacgcagt ccaggccacg gtgaactcgc tcgtcgagag gctcctcgtg
     1561 gggcgcaggc tcttcgaggt ggttcactcc ggcacagacg tcccatccgg catcgtgtgg
     1621 gcgcagacgc aggcccatgg cggagacaag gccgagggcc cgaccgtgga ggtcagtacg
     1681 gcccagatcg ccgctcccag cacgcgtctg tgggcggcgg tggagatcga cgagtctgag
     1741 atcgacctcc gagcatgcat agcccggtct agcgccctgg tcgatgtggc ccggcagatg
     1801 gcgctccagg aggaccgggt ggtcttcaag caggtgtggg agcggagctc ccggctgccc
     1861 gcgacccacg gtggtcgctc tgctgatggg gatcccgccg cgacagcgct caccacggcg
     1921 ttaggcacgc tccgcggggc cggccatcgc gggggcgtcg ctctgctact cgcggctgga
     1981 gatggcttcc gcgacatcga gtcggagcta gctcggacgg gctgcatcgc tcgggtggcg
     2041 cccctgttcg ctctcgggat ccacgccgtt cccctcatgg cgtctgggga cgcgctggta
     2101 gtcgcccccg aggggtccgg tgccagcatc accgtgggcc agaactacgc cgtgtcgtgc
     2161 attgagtgca gcgggccgcg gctgaggttc gggatctgta cgacgtttgg cgtctctccc
     2221 ggcgctccag ccgcgcagtg ccggttgagc gtgcccgaac cgccggctcc cggcggcgcc
     2281 cagcggaagc gcgggtagac acggcccctc gggccattgg ccttcctgcg cgcctcgtgg
     2341 ccgcctgtgg tatcgttccc ctgacattct ggggaggtac ccgacgtggc ccgacaccgt
     2401 ttgctgctgc gctgcctgct tggtcttgcc gtactctgca gtgtcgtctc ttccgtctcg
     2461 gcggaggcga cgctgcgtgt caacaccgac cggcctgacg ccatctacgc gtgcggggat
     2521 gatgccacat tcgccgtggg tctccagcgc gatggcggcc ccgtggtcga ggccaccgtc
     2581 cagtatcggc tgaccctcga cggcggcaag ctcctcggcg agggcgagct cgcggttcgc
     2641 gacggccgag cagaggtgcg cgcgacgctg gcggagcccg gcttcgtgcg cctctcggtg
     2701 acattcgccg gggaggacgg ccacatcgac gccatggccg cggcggcctt cgaccccgag
     2761 cacatcgtgc catcggccga cgagccggcg gacttcgtcg agttctgggc gaggcagaag
     2821 gccgagttgg ccaccatcgc tcccgacccg cggctcgagg agcgggccga cctgtccagc
     2881 gaccagcgga cggtctacaa gatcagcctg gcgaacatcg gcggcagccg cgtctacggc
     2941 tggctggcag tgcccaagtc cccgggcccc tatcccgccg tgctgaccgt cccctgggca
     3001 ggtgtgtacc cgacacccgt cgggctcatc gactgggcgg accgaggctt cctcgccatg
     3061 gccatcagcg cgcacgacta cgatgtcgac atgccggcgg aggagtacaa gaagctcgag
     3121 gagggcccgc tcaagggcta tccccatcag gggcgcgaga gccgcgagac gggttacttc
     3181 cgccgggtgt tcctcggatg cgttcgggcc gtggactacc tgacatcccg cgaagactgg
     3241 gacgggacgt cgctgatcgt caccggttcg agccaggggg gagggctatc gctggtcacg
     3301 gcggggctcg accgccgagt gacggccctg gcggcgaatg tgccggcgct gtgtgaccac
     3361 ctcgggtaca aggcgggcca ccagccgggc tggccgcgcc tggtgactga gggcgccgcc
     3421 gaccaggaac aggctgcggg ctacttcgat gccgtcaact tcgcgcgcca cgtggcctgc
     3481 ccggccattg tcggagtagg cttcatcgac accgtgtgcc cccccacggg cgtctacgct
     3541 gcctacaacg tgctcggcgg cccgaagcag atcgtcccga cgcccttaat gggacactca
     3601 cagagcgagg tgtatggtgc cttgctccat cgctggatcc ccgagcaggc cggccgagcc
     3661 gcccggtaac gcgaccgtca tcactccggc ctcacacagc tccctgcccg ccacaagcga
     3721 gtgtcccccg ctgtgctgcg cgcacgcgag ggcggcgctc cgcaggtatg agagcggcag
     3781 tgacggtgag tgctgcgtgc gccctgacat tggcgtccgg cgggtcctct ccgcgcgcca
     3841 acccgccact tcgttgggaa caatgctgag tgggccgctg tttcacggaa gaactacctg
     3901 gaagcggcgc gcggccgaac ttcggcgcaa cggccctcgc ggcagggcat tgtgccctgg
     3961 cgccacggtt gcctgccccc aggcgggggt agcgtgcgtc ctggtgcgcg tggggaggac
     4021 cctgccgtgg atcttcgggc gatcctggac cgttccgaca tccccgagga cgtgaggcga
     4081 gccctgcagg cgcacctcgc ggacccgtcc acccgagagg accggctcta tcgcgagctc
     4141 ttcgagaggt ccagcgatgc ggttttcctg ctgcacggga cccgcttcat cgactgcaac
     4201 ctcgccgcc
//
LOCUS       DAOSPL010000188         4208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21157_length_4208_cov_3.343848, whole genome shotgun sequence.
ACCESSION   DAOSPL010000188 DAOSPL010000000
VERSION     DAOSPL010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21157_length_4208_cov_3.343848"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PLQ54_04805"
     CDS             <1..978
                     /locus_tag="PLQ54_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOS92608.1"
                     /translation="YTYPKTLRDEIATVAPDYRVDVEEFRTEDKHGLLRQLYDVTSGH
                     FRVVEHLMATKSWDLFWFVEMGTDRLYHGFWRYFADDHRLFEPGNPFEQAALEYHVYL
                     DQRIGAVLKHVDDDTLVLVVSDHGAASMVGGIAINDWLIREGHLVLRRPAESPRRLTA
                     DDVDWARTTAWGEGGYYGRIMLNVRGREPEGLVDPNEYERVRDEIARGLESLGDDQGQ
                     PMGTRALKPEDVYRQVRGIPPDLIVYFGNLRWRSVGTVGNPGVHVFDNDTGPDDANHA
                     EEGLLIIHDPWDPRPGELQGATLYDVAPTVLERLGLPVPADMIGRPLSW"
     gene            993..1532
                     /gene="cysC"
                     /locus_tag="PLQ54_04810"
     CDS             993..1532
                     /gene="cysC"
                     /locus_tag="PLQ54_04810"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015219735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOS92609.1"
                     /translation="MEHKGFTVWLTGLSGAGKSTIAEAVAAELRGRGFKVEVLDGDVV
                     RTNLSKGLGFSKEDRDTNIRRIAFVAHLLSRNNVVAITAAISPYRAIRDEARAMIGNF
                     VEVFADAPLAVCEERDTKGLYAKARSGELKGFTGVDDPYEAPLNAEVVCHTDKESLDE
                     SLAKVMAKLEELRYLEQVG"
     gene            1633..2808
                     /gene="sat"
                     /locus_tag="PLQ54_04815"
     CDS             1633..2808
                     /gene="sat"
                     /locus_tag="PLQ54_04815"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HOS92610.1"
                     /translation="MSSSLIAPHGGRLVAPLVPPSERAAKLSHAAGLPAVRLTSREVS
                     DLIMIGMGAFSPLAGFMGEADYRGVVTDMRMADGTLWPIPITLAPAAGVDGITPGQEV
                     ALVDDDSGEIMGTMRVEELFSYDKRAEARHVFRSEDEAHPGVAKVYAQGDTCLAGPVE
                     VLSEGGYPERFPEYARPAEVRALFAERGWQTVAAFQTRNPLHRSHEYLTKVALEISDG
                     LFIHPIVGKLKEGDIPAEVRQRCYQVLLDSYYPEDRVLLRVYPMEMRYGGPREAVLHA
                     IIRQNFGCTHLCVGRDHAGVGKYYGPFDAQAIFDEIPPGDLLIRPLKFDNTFWCRACQ
                     SMASSKTCPHPPEHHLSISGTKLREMLSAGELPPEEFSRREVLQVLVEYYRSMAAGA"
     gene            2945..>4208
                     /locus_tag="PLQ54_04820"
     CDS             2945..>4208
                     /locus_tag="PLQ54_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOS92611.1"
                     /translation="MAGRATHWPKVIIIGLDGGTLDLIRPWAKRGELPNLQSLMRSGV
                     SGPLTSTVPPITPVAWSAFLTGQPPERHGVYDFVERLPQSYRTRVTHANSRRCPSFWQ
                     TLSAAGLVVGALHTPFTYPPERVRGFMVSGFDAPRLDRSAVSPPEAMDCIADLCRGYV
                     HGSLPMRPRATYADRAGEKARRLVAMALRLQSRWPCDVLMITASSTDHVQHHFWPEGQ
                     AADQVVRAGGEILATYRSVDAAIGELLSAVRGPETTVLVMSDHGAGPLMGHVNQTRVL
                     AEAGLLRLRGAAAGSRSARASVRAVRGIRRLFRSGLARPLKPLALRLMPESAKARVAE
                     TELDAIDWSRTRAFAWGTYGKVQVNLAGNILVSKYAIPHLIRTKGKLVFFASSSYTKG
                     REGYSVYSATKAAIVNLMQALADELSGQV"
BASE COUNT          685 a         1385 c         1440 g          698 t
ORIGIN      
        1 tacacgtacc cgaagacgct gcgggacgag atcgcgaccg tggctccgga ttaccgcgtg
       61 gatgtcgaag agttccgtac cgaggacaag catggcctgc tccggcagct ctatgacgtg
      121 acgagcggcc atttccgggt ggtcgagcac ctgatggcga cgaagtcgtg ggacctgttc
      181 tggttcgtcg agatgggcac ggaccggctt taccacggct tctggcggta cttcgccgac
      241 gaccaccgcc tctttgagcc gggcaacccg ttcgagcagg cggcgctgga gtaccacgtc
      301 tatctcgatc aacgcatcgg cgccgtcctc aagcacgtcg acgacgacac actggttctc
      361 gtcgtctccg accacggtgc ggcgagcatg gtcggcggca tcgcaatcaa cgactggctc
      421 atccgcgaag gacacctggt cttgcgccgg ccggccgagt cgcctcgtcg cctcaccgcc
      481 gatgatgtgg actgggcgcg aaccacggcg tggggtgagg gcggctacta cggacgcatc
      541 atgctcaacg ttcgcggtcg agagcccgag gggctcgtcg atcccaacga gtacgagcgg
      601 gtgcgcgacg agattgcgcg cggtttggag tctctgggcg acgatcaggg gcagccgatg
      661 ggcacccgag ctctcaagcc cgaggacgtc taccgccagg tccgagggat cccacccgat
      721 ctgatcgtct acttcggcaa tctgcggtgg cgctccgtcg gcaccgtggg gaatcccggg
      781 gtgcacgtct ttgacaacga cacggggccc gatgacgcga accacgccga agagggcctg
      841 ctgatcatcc atgacccctg ggacccgcga ccgggcgagc ttcagggggc aacgctgtac
      901 gacgtagcgc ctacggtact ggagcgacta ggtctgccgg tccctgctga tatgatcggc
      961 agaccattga gctggtaggg cgaggagtgg ctatggagca caaagggttc accgtgtggc
     1021 tcacggggct gtcgggagcg gggaagtcga ccatcgccga ggcggtggcg gccgagctgc
     1081 gcggccgtgg tttcaaagtc gaggtgctgg acggcgatgt ggtccgcacc aatctgtcca
     1141 agggcctggg cttctcgaaa gaggatcggg ataccaacat ccggcgcatt gccttcgtcg
     1201 cgcatctgtt gtccaggaac aacgtggtgg ctatcacggc cgccatctcg ccctaccgcg
     1261 ccattcgcga cgaggctcgg gcgatgatcg ggaacttcgt cgaggtgttc gccgatgcgc
     1321 ccctggcggt gtgcgaggag cgagacacca aggggctcta cgcgaaggcc cgctctggcg
     1381 agctgaaggg cttcaccggc gtcgacgacc catatgaggc gcccctcaac gccgaggtcg
     1441 tgtgccacac cgacaaggag tcgctggacg agtcgctggc aaaggtcatg gcgaagctcg
     1501 aggagctgcg ctacctggag caggtcgggt agcggcaggt agcgctcctc ggttacaccc
     1561 gcatggcccc ctcttccctc gggagaggac ccgggagagg gggccggccc gcaactgaaa
     1621 ggaaccgcgc ccatgagctc ctcgttgatt gccccgcacg gcgggcgtct ggtggcgcct
     1681 ttggtccccc cgtccgagcg cgcagccaag ctcagccacg cggcggggct ccctgccgtc
     1741 cggctgacct cgcgcgaggt ttcggacttg atcatgatcg gcatgggcgc cttcagtccg
     1801 ctggccgggt tcatgggcga ggccgactac cgtggcgtgg tcaccgacat gcggatggcc
     1861 gatggaacgc tgtggcccat cccgatcacc ctggcgcccg cggctggtgt ggacggcatc
     1921 accccgggcc aggaggtcgc gctggtcgac gatgactcgg gcgagatcat gggcaccatg
     1981 cgggtcgagg agctcttctc atacgacaag cgggcggagg cccggcatgt gttccgcagc
     2041 gaggatgagg cacatccggg agtggccaag gtgtacgcgc agggcgacac ctgcctggcc
     2101 gggccggtcg aggtgctgag cgagggcggc taccccgagc gcttccccga gtacgcccgg
     2161 cccgccgagg tccgggcgct gttcgccgag cgcggatggc agacggtggc cgcgttccag
     2221 acgcggaacc cgttgcaccg ctcgcacgag tacctgacga aggtcgctct cgagatcagc
     2281 gacggactct tcatccaccc gatcgtcggg aagctcaagg agggcgacat ccctgccgag
     2341 gtccgccaac gctgctacca ggtgctgctc gactcgtact accccgagga ccgtgttctg
     2401 ctgagggtgt accccatgga gatgcgttac ggcgggccac gcgaggccgt tctgcacgcc
     2461 atcatccggc aaaacttcgg gtgcacgcac ctctgcgtgg gccgcgacca tgccggagtg
     2521 gggaagtact acggtccatt tgacgcccag gccattttcg atgagatccc gccaggcgat
     2581 ctcctgatcc ggccgctgaa gttcgacaac accttctggt gccgcgcatg ccagagcatg
     2641 gcgtcttcga agacctgtcc gcaccccccg gagcatcacc tgagcatcag cgggaccaag
     2701 cttcgggaga tgctcagcgc cggtgagctc ccgccggagg agttcagccg ccgcgaggtg
     2761 ctccaggtgc tggttgagta ctaccgatcc atggcggctg gcgcgtagcg gcgcctcgcc
     2821 tccagcacct taccgaacat tgctcggctt cgctcgtccg tagacgagag cacgcacagc
     2881 cgcgccgggt ctgggcgggg cggcctcgtt ggggcccgtg ctccgggggg cgaggcatgg
     2941 gcctatggcg gggcgcgcga ctcactggcc caaggtcatc atcatcgggc tggacggcgg
     3001 tacgctggac ctgatcaggc cctgggcgaa gcgcggtgaa ctgccgaacc tccagagcct
     3061 gatgcgttcc ggcgtctccg ggccgctaac gtccacggtt ccccccatca cacctgtggc
     3121 ctggagcgcc tttctcaccg gccagccgcc cgaacggcat ggcgtgtacg acttcgtcga
     3181 gcgccttcca cagagctaca ggacgcgggt tacccacgcg aacagccgcc gctgtccgag
     3241 cttctggcag acccttagcg ccgcgggcct cgtggtcggt gcgctgcaca cgccgttcac
     3301 ctatcctcca gaacgggtac gcggcttcat ggtgagcggt ttcgatgccc ctcgtctcga
     3361 tcgctcggcg gtgtcgccgc ccgaggccat ggactgcatc gccgacctgt gccgcggtta
     3421 tgttcacggc tcgctgccga tgcgcccaag ggccacctat gcggatcgcg cgggcgagaa
     3481 ggcccgccgg ctcgtcgcca tggcgcttcg cctccaatcg cgatggccct gcgacgtgct
     3541 gatgatcacg gcctcgtcca ccgaccacgt tcagcatcac ttctggcccg agggccaggc
     3601 ggccgaccag gtcgtacggg ccggcgggga gattctggcg acctaccgaa gcgtcgatgc
     3661 cgccatcggc gagcttctgt cggcggtccg cgggcccgag accacggtcc tggtgatgtc
     3721 tgaccacggc gcgggtccgc tcatggggca cgtgaaccag acccgggtgc tggccgaggc
     3781 cggtctactg cggctccgtg gcgctgcggc gggctcgcgg tcggcgcggg cctcagtgcg
     3841 ggccgtgcga ggaatccggc gcctgttccg gagcggactg gcgaggcccc tcaagcccct
     3901 ggctctgcgc ctcatgcccg agtcggccaa ggcgcgggtg gccgaaaccg agctcgatgc
     3961 catcgactgg tcgagaacgc gggccttcgc atggggtacc tatggcaagg tgcaggtgaa
     4021 cctggccggc aacatcctgg tctccaagta cgccatcccg cacctgatca ggaccaaggg
     4081 caagctggtc ttcttcgcct ccagctccta taccaaaggg agagaggggt actcggtcta
     4141 cagcgccacc aaggcggcca tcgtgaacct catgcaggcc ctggccgacg agctcagcgg
     4201 gcaggtgg
//
LOCUS       DAOSPL010000189         4206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21173_length_4206_cov_3.418453, whole genome shotgun sequence.
ACCESSION   DAOSPL010000189 DAOSPL010000000
VERSION     DAOSPL010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21173_length_4206_cov_3.418453"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PLQ54_04825"
     CDS             complement(<1..673)
                     /locus_tag="PLQ54_04825"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOS92612.1"
                     /translation="MRLLLAIWAAKLVAFACRALGRGGGTSLPGLVALRIHPRADSCL
                     AARLPRGCVVITGTNGKTTTAKMVATVLRTAELRVVRNVSGANLRRGAVATLVGATGP
                     LGRLDADIGLFELDEATIPEMVRALRPRIVAVTNLFRDQLDRYGELDTTAKRMNQAFR
                     ESLQREQTLVLNADDPLVAELAEGSPARVVRFGIDAPQAAGDADSHAADTKTCPICGG
                     DYDYAA"
     gene            1154..3001
                     /locus_tag="PLQ54_04830"
     CDS             1154..3001
                     /locus_tag="PLQ54_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOS92613.1"
                     /translation="MASEELGFLHRNKERITRHWRDYVINHVSSRIFDYPDFAEDRLL
                     DRLFVYITQALEQNHYYDLNRTFDRMLDTGHRHFISIGDIRGVLFGFIDATQSVLDEL
                     EDDTALTRYLKRRLTHFAADLRQVYSERLAGSSIEQLLRHRSDIEEKWVRDLPSEAVS
                     RHLVVVSEDRRRKFVRDTFDVAIAFLQGNERAPVEIGDDDGDTVTHVDAYLRDVIDYF
                     EPRGFAISHIERAIRHLEEICEPILFRFYEDEPHLYRRALWVIHSAVNDLALAFSEGY
                     NQRMMKNYYNEVSIMLHRIKNKLTAVPTSLLTIMTNTYEDIVVEGDVLSAEDGRLFAE
                     LQQLRQKALKHGLEAIELGLERLGAQADLAETLADVVAKLEAAKACFDELKAFTSENR
                     ERVKAITRKLDADAVERVDEFLRDALEGGQTTTDLTRELQDIQNELYRREPPVWRPLD
                     LNQLVQAAYDESIVDAKGKNIRYTLELCEGGTQVFGVERELKRPFAQVINNAIKYTPE
                     NGEVSVSTHVGDGYVTVSVKDSGIGIPAGEENMVFGLCERCSNAKEFNKVGSGTGLYH
                     DRKTVLQHNGDMWVESEGENKGSTFYIRLPIYKGDGSDGEEEARLADQP"
     gene            3128..>4206
                     /locus_tag="PLQ54_04835"
     CDS             3128..>4206
                     /locus_tag="PLQ54_04835"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOS92614.1"
                     /translation="MACVAPAAAARRAEDELVVWLVSNNSGFRALCREFERRSGLRLV
                     VALNTRGMDPQKILCGIAGGDPPDVLYQDRFAVGGFAARGAFMPLDDFIRRGSLNWRS
                     FYEGTRAEATYQGQVYAIPWQCDNRDLYYNKDLLSRARLVNSRGEPQPPRDWAELKDY
                     AVRLTEKDGDRFTRIGFDPNYGNVWLYMYAWQAGGEFMSPDGATCTLNAPENVRALEY
                     MLDVYDAIGGREAVMKFSSTFEAGENDPFFAGKVAMKVDGNWVLDQISRYAPNLDFGV
                     APAPMPRRTDTPISWSGGFAWTIPVGSSKPEKAWDFIEFCMSDEGLRLYHEAQGRESQ
                     SRGQPYVPEFSARQDPIATMLTQCHV"
BASE COUNT          771 a         1396 c         1337 g          702 t
ORIGIN      
        1 gcgcagcgta gtcgtagtcc ccgccgcaga tggggcatgt cttcgtgtcg gctgcgtgcg
       61 agtccgcgtc tcctgccgcc tggggtgcgt cgataccgaa ccgcaccacc cgcgccgggc
      121 tgccctcggc cagctcggcc accagtggat catcggcgtt cagcaccagc gtctgctccc
      181 gctgcaggct ctcgcggaat gcctggttca tgcgcttggc ggtcgtgtcg agctcgccat
      241 accggtcgag ctggtcgcgg aagaggttcg tcacggcgac aatgcgcggc cgcagcgcgc
      301 gcaccatctc gggtatggtg gcctcatcga gctcgaacag cccgatgtcc gcatcgagcc
      361 gacccagcgg cccggtcgcg ccgacgagtg tcgccacggc cccccgccgc aggttggctc
      421 cagagacgtt ccgcaccacc cggagctccg ccgtccggag gacggtggcg accatcttgg
      481 ccgtggtggt cttgccgttg gtcccggtga tcactacaca gccccgcggc agccgcgcgg
      541 cgaggcacga atcggcacga gggtggatcc gcagggccac taggccgggc aggctcgtgc
      601 cgccaccgcg gccgagggcg cggcacgcga acgcaaccag cttggcggcc cagatggcca
      661 gaagcagacg catgggcgcg cgactccagt cctcttgcag gttgcgccca tcttaccatc
      721 cgcaaccagc cccggggccc gccgccccga tcgggcgcag tggacgcgag atgcagatgc
      781 gagccctcca gaagagagcc gcgagcggtc gtgcggccga ggctggcgtc tcgccgcagg
      841 cctccgattc cttggattta agtaaggaac gcaaacgccc gcgcaaagcg cgttgacccc
      901 ccacccccaa gtgggtagaa tccggatgaa tggggccttc aggtagactg acgccaacac
      961 ggtgaacagg cgggggaacg ggcccggcct ggtgttgcac ggttggcgcc gggacgccaa
     1021 gccttggggc gcccaacgcg accatattgg caagctctca ttgtgctcca ccgcttatgc
     1081 ccaaggtctc gttgagcacg ggaacctcca tgcttggccc gaggcgtcca cgcgtatcag
     1141 cgggggttca tggatggcgt ccgaggaact tgggtttcta caccgcaaca aggaacgcat
     1201 cactcggcac tggcgagact acgtcatcaa ccatgtcagc tcgcggatct tcgactaccc
     1261 cgacttcgcc gaggaccgcc tgctggaccg cctatttgtc tacatcactc aggccctcga
     1321 acagaaccac tactacgacc tcaatcgcac gttcgaccgg atgctcgaca ccggacaccg
     1381 ccacttcatc agtatcggcg acatccgcgg cgtcctgttc gggttcatcg atgccaccca
     1441 gagcgtgctg gatgagctcg aggatgacac ggcccttacg cggtacctca agcgccggct
     1501 cacgcacttc gccgccgacc tccgccaggt ctactccgag cgcctcgccg gctccagcat
     1561 cgagcagctc ctccggcacc ggagcgacat cgaggagaag tgggtccgcg acctgccctc
     1621 tgaggccgtc agccggcatc tggtggtcgt gagtgaagac cggcggcgca agttcgtgag
     1681 ggacactttc gacgtggcca tcgccttcct ccagggcaac gaacgcgcgc cagtcgagat
     1741 tggcgatgac gatggcgaca cggtcacgca cgtcgatgcc tatctgcgtg acgtgatcga
     1801 ctacttcgag ccccgcgggt tcgcgatctc gcacatcgaa cgagccatcc gccacctcga
     1861 ggagatctgt gagccgatcc tgttccgctt ctacgaggat gagccccatc tgtaccgtcg
     1921 ggctctgtgg gtcattcaca gtgccgtgaa cgacctggct cttgcattca gtgagggcta
     1981 caaccaacgc atgatgaaga actactataa tgaagtctcc atcatgctgc atcgcatcaa
     2041 gaacaagctc acagccgtac cgacgtccct gctcaccatc atgaccaata cctatgagga
     2101 tatcgtcgtc gagggcgatg tcctcagtgc cgaagacggc aggctgttcg ccgaactcca
     2161 gcagctgcgg cagaaggcgc tcaaacacgg gctggaggcc attgagcttg gcctcgaacg
     2221 gctgggggcc caggcggatc tggccgagac gctcgctgac gtggtcgcga agctggaggc
     2281 cgccaaggcc tgcttcgacg agctgaaggc cttcaccagc gagaaccgtg agcgtgtcaa
     2341 ggccatcacg cggaagctcg atgccgatgc ggttgagcgg gtcgacgagt tcctccgcga
     2401 cgccctcgag gggggacaga cgaccaccga cctgactcgc gagctccagg atatccagaa
     2461 cgagctctat cgccgcgagc cgccggtgtg gcgccccctc gacctcaacc agctcgtgca
     2521 agcggcctat gacgagtcca tcgtcgatgc caaaggcaag aacatccgct acacgcttga
     2581 gttgtgtgag ggcgggacgc aggtgttcgg tgtcgagcgg gagctcaagc ggccattcgc
     2641 ccaggtcatc aacaatgcga tcaagtacac acctgagaac ggcgaggtca gcgtttccac
     2701 tcacgtgggc gatggctatg tgaccgtcag tgtcaaggac tctgggatcg gtatccccgc
     2761 gggcgaggag aacatggtgt tcggcctgtg tgagcgctgc tcgaacgcca aggagttcaa
     2821 caaggttggc tcgggcaccg ggctctacca cgatcgcaag accgtcctcc agcacaacgg
     2881 ggatatgtgg gtggagtccg aaggggagaa caagggatcg acgttctaca tccgtttgcc
     2941 catctacaag ggtgacggat ctgatggcga ggaggaggcc cggctcgcgg accagccctg
     3001 atccgtgccg ggctggcgcc gagtgtcccg cccacgccat atccgtacgc cgcatgccgc
     3061 gccgggccgg tgtcaccgcc ctgccgtgcg cccatggctc acgtgtgcag ccctggcggc
     3121 cgtcgccgtg gcttgcgtcg cacccgccgc cgcggcccgg cgagccgaag acgaactggt
     3181 cgtctggctg gtcagtaaca acagcggctt ccgcgccctc tgccgcgagt tcgagcgccg
     3241 cagtggcctc cgcctggtcg ttgccctgaa cacccgcggg atggatcccc agaagatcct
     3301 gtgcggcatc gccggcggcg acccgcccga cgtgctctat caggaccgtt tcgccgttgg
     3361 cgggttcgcg gcacgaggag ccttcatgcc gctggacgat ttcatccgtc gcggcagtct
     3421 caactggcgg tcgttctacg agggcacccg ggcagaggcg acttaccagg gccaggtcta
     3481 tgccatccca tggcagtgtg acaaccggga cctctactac aacaaggacc tgctgtcgcg
     3541 agcccggctc gtgaactcgc gcggcgagcc ccagccaccc cgggactggg ccgaactcaa
     3601 ggactacgcc gtgaggctca ccgagaaaga cggagatcgg ttcacgcgga tcggcttcga
     3661 ccccaactac ggcaacgtgt ggctctacat gtacgcctgg caggccggtg gcgagttcat
     3721 gagccctgac ggagccacat gcacgctcaa cgcgcccgag aacgtgcgag ccctggagta
     3781 catgctggac gtctatgacg ccatcggcgg ccgcgaagcc gtcatgaagt tcagttccac
     3841 gttcgaggcg ggcgagaacg acccgttctt cgcgggcaaa gtggccatga aggtggatgg
     3901 caactgggtg ctcgaccaga tcagccgcta tgcgccgaac ctcgacttcg gcgttgcacc
     3961 cgcgccgatg cccagacgga ctgacacgcc catcagctgg tccgggggct tcgcctggac
     4021 gatcccggtt ggttcgagca agcctgagaa agcctgggat ttcatcgagt tctgcatgag
     4081 cgacgaaggc cttcggttgt accacgaggc gcagggacgc gagtcgcaat cgcgtgggca
     4141 gccctacgtt cccgagttct cggcgcggca ggaccccatc gccacgatgc tcacccagtg
     4201 ccatgt
//
LOCUS       DAOSPL010000190         4192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21272_length_4192_cov_3.636935, whole genome shotgun sequence.
ACCESSION   DAOSPL010000190 DAOSPL010000000
VERSION     DAOSPL010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21272_length_4192_cov_3.636935"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..510
                     /locus_tag="PLQ54_04840"
     CDS             61..510
                     /locus_tag="PLQ54_04840"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOS92615.1"
                     /translation="MRHSSVLQRREAFRARPVPYTAPVAIRIDGLPAPLGGHVLDLSC
                     AGMRVALPCRAHTTMRSIAPGSAGVAYLAFLDPSQAWDSSGQACDGVPTVGARVVWRE
                     LASTRSELRVALALSMPNPSLRSRLARYIQDCDRETLHVRRPISRHR"
     gene            1211..1894
                     /locus_tag="PLQ54_04845"
     CDS             1211..1894
                     /locus_tag="PLQ54_04845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS92616.1"
                     /translation="MVEDVTAGSLTELLGRASIRTYALARELGGRIAHTAIREIVENL
                     VHAGCAGATISILDGGHRIVVSDQGCGIPDKERCMLPGFTTAAPWMRQYIKGVGSGFV
                     VASESMSRLQGSVRVEDNLGRGAVVTLAEPLQAPVDASAPGAPRLAAGLRPGFHLNER
                     QKRALLVLAESQHAGPSVLAKELGVALATAHREMGRMEAMGLVQYVGKGKRVLTAEGL
                     ELVRSFTGT"
     gene            1987..3408
                     /gene="dnaA"
                     /locus_tag="PLQ54_04850"
     CDS             1987..3408
                     /gene="dnaA"
                     /locus_tag="PLQ54_04850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOS92617.1"
                     /translation="MHRRESGGLTAELDRAWHGAIRDIGAQMGDWARNAYLDNLRPVS
                     LEGPILTIGATNEFARSRLEERYSRTFESVLSDQLRRAIRVKFVMLRPAASPTVEPEK
                     GPTPAAPEVSSASGSDMLGFSSLALNPRYTFDCFVVGSSNRLAHAAAQNVANSPGRTY
                     NPLFIYGGVGLGKTHLMQAIGHHALQANPGIRVVYISGETFLNHVVTAIRDGRTDAFR
                     QAYRTVDLWLVDDIQFIASKEQSRTEAEFFHTFNALYETNKQIVISSDRTPQDLQIMD
                     DRLRSRFEMGLLTDIRMPDEETRFAILQRKASSEQARVPDEVLKYIADMVRSSVRALE
                     GALTRVIASASLTGSNITLETAREWLADYASRDGHVRITPRQVRDAVARHFGLKPEEL
                     AGKRRDRAVVVPRQIAMYLARELGQASTVQIGTVFGGRDHSTVLHACQKVRDMLETDT
                     GAAQLIADITSAIRSPDSLGEGD"
     gene            3692..>4192
                     /gene="dnaN"
                     /locus_tag="PLQ54_04855"
     CDS             3692..>4192
                     /gene="dnaN"
                     /locus_tag="PLQ54_04855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOS92618.1"
                     /translation="MKIRCAKDELYEAIQTVQRAVSGRSTLPILNNVLLEADDDTLCL
                     VAYDLEFGIECTLAVKVEARGGATIPARILAEVVGSMPEAEITIEVDERQVVTITCGG
                     SRYEIHGLPAEEFPRLPLLTEGARALVNRARFRQLIRSTSFATSVDETRPILTGVLTQ
                     LTADTIM"
BASE COUNT          729 a         1346 c         1342 g          775 t
ORIGIN      
        1 tctcatgagc cgcgctcctg gcacggccgg caaccctgag tcgagcgaca aggcggcgac
       61 gtgcgacact cttcagtcct ccagcggcgt gaggcgttcc gggcacgtcc cgtgccctac
      121 acggcaccgg tcgccatccg gatcgacggc cttcccgcgc ccttgggcgg ccatgtgctt
      181 gacctgagtt gcgcgggcat gcgggtcgcc ctcccctgtc gagcccacac gacgatgcgg
      241 tcgatcgccc cgggatcggc cggtgtggcc tacctggcct tcctcgaccc gtctcaagcc
      301 tgggactcct cgggccaggc gtgcgatgga gtgcccaccg ttggcgcgcg ggtggtctgg
      361 cgcgaactcg caagcacgcg gtccgagctt cgcgtggccc tggccctcag catgccgaat
      421 ccctccctcc gatcgcgcct ggcccgctac atccaggact gcgatcgcga gacccttcac
      481 gtccgccgcc ccatttcccg ccatcgctga cggtcctctc tggcccgggg ggaaagcggg
      541 cccatcggac tgcaggagcg ccctctgatc ctgtggctga accacggcct ttgcgtccat
      601 tgcagctccc taatgggtac gctagaatcc gccacctctg gatggggagc ttggggatga
      661 gtccgtacac tgccgtggaa acgtcgtgct tgccgccggt ccggcctgcg tatgggagtc
      721 accttgccaa cgatgtggcg acgggccaaa gattcccgga cagaatcttt tccacacctg
      781 tggataactc tgtggaaaac cttccaggtg tgcgacgatc tgcctccaag gcttgtaccg
      841 ccagcgttgg aggccctgta cggtggcccg catccttcgg ggcgccgggc gcggtactcg
      901 gtcgcaccgt ggtacgggtt atccacagga ttgccatggc cttccgagcc gtgaaaggca
      961 agcaggtgac ctttcggtcc gcgtgcgaac ccgcccctgg gccggaaagc gtgccgtcca
     1021 ataggtacgg tagcccaagg tacgccgggg aggtcttggc gtgagagtcc gtatccgaaa
     1081 gcctttcgct ccccacggaa gaacctggaa acatgcccgg ctgctttccc gtcctggccc
     1141 tccgatgggc cggaaggatt cgggttcctc catccgaatt gcggtctacc gcgaggggct
     1201 ggaggctccg atggtcgagg atgtcacggc cggatcgctc acggagttgc tgggacgcgc
     1261 cagcattcgg acttacgcgc tggcccggga actcgggggc cggatcgctc acacggccat
     1321 ccgagagatc gtggagaacc tcgtccatgc ggggtgtgcc ggcgccacaa tcagcatcct
     1381 ggatggtggg caccggatcg tcgtgtcgga ccagggatgc ggtatcccgg acaaggagcg
     1441 ctgcatgttg ccagggttca cgacggctgc tccgtggatg cggcagtaca tcaaaggcgt
     1501 gggctccggg ttcgtggtgg cgtccgagag catgagccgc ctccaaggga gcgttcgggt
     1561 cgaggacaac cttggtcggg gcgccgtggt cacgctcgcc gaaccgctgc aggctccggt
     1621 cgatgcgtcc gctccgggtg cgccccgact ggctgccgga ctccgcccgg gcttccacct
     1681 caacgaacgg cagaagcggg cgcttctggt gctggccgag agtcagcatg caggcccttc
     1741 agtcctcgcc aaggagctcg gagtcgcctt ggcgacggct catcgcgaga tggggcgcat
     1801 ggaggccatg gggctggtgc agtacgtggg taaggggaag cgcgttctca ccgcggaagg
     1861 tctggagttg gtgcgctcat tcacggggac atgaccccag gttaacgagg gtggggtgag
     1921 cggcgagtgg tgcggggcgg atccgggtac gggtccgggt tgtatgggac tgaggagctg
     1981 agcgggatgc atcgcaggga gtcaggaggc ctgacggcgg agctggatcg ggcctggcat
     2041 ggcgcgatca gggacatcgg tgcccaaatg ggtgattggg cacggaacgc ttacttagac
     2101 aacctgcgtc cggttagcct tgagggcccc attctgacca tcggcgccac caacgagttc
     2161 gcccgatccc gcctcgagga acgctattcc cgtacgttcg agtccgtgct ctccgaccag
     2221 ctccgccggg ctatccgcgt gaagttcgtc atgctccgcc ccgccgcctc acccacggtc
     2281 gaacccgaga aggggcccac cccggcggcc cccgaggtct cctcggcctc gggcagcgac
     2341 atgctcgggt tcagctctct cgccctcaac cctcggtaca cgttcgactg cttcgtggtg
     2401 ggcagcagca accgtttggc ccatgccgcg gcccagaatg tggccaactc gcccggacgc
     2461 acatataacc ccctcttcat ctacgggggt gttgggctcg gcaagaccca cctgatgcag
     2521 gccatcggac accatgctct tcaggccaac cccggcatcc gcgtcgttta tatatctggg
     2581 gaaacatttc taaatcacgt ggtcactgcc atccgcgacg gccggaccga cgcgtttcgg
     2641 caggcctatc ggaccgtgga cctgtggctc gtggacgaca tccagttcat tgcctccaag
     2701 gagcagtccc gcacggaagc agagttcttt cataccttca atgcactcta cgagaccaac
     2761 aagcagattg tcatatccag tgaccgtacc ccgcaggatc tgcagatcat ggatgatcgc
     2821 ctgcggtcgc gttttgagat gggactcctc acggatatca ggatgcccga tgaggagacg
     2881 cgcttcgcca tcctgcaacg caaggcatcc agcgaacagg cacgcgttcc cgatgaggtc
     2941 ctcaaataca tcgctgacat ggtccgatcc agtgtgcggg ctctcgaggg cgctctgaca
     3001 cgggtcattg ccagtgccag tctcacggga tcgaacatta cgcttgagac cgcacgggaa
     3061 tggctggctg actatgcgtc gcgcgacggc catgttcgga ttacgccccg ccaggttcgg
     3121 gatgctgtgg cccggcactt tggcctcaag cctgaggaac ttgccggcaa acggcgcgat
     3181 cgcgcggtcg tcgtgccgcg gcagatcgcc atgtacctcg cccgagaact cggccaggcc
     3241 tcgaccgtgc agatcggcac cgtcttcggc ggccgcgacc actccaccgt gctccatgcc
     3301 tgccagaagg tgcgcgacat gttggagacg gacaccggag cggctcaact tattgccgac
     3361 atcacatcgg caatccgatc accggactca ctcggcgaag gcgactgaat catccacatc
     3421 cgcaccgtgg aaacgctgtg caccatgcgt ggatatctct atcgggaggg caaccggtgg
     3481 ataccgggga aagaacttga gggttatcca gagggttttc cacctgccat accgcatcct
     3541 agcgtgcttg cggccagtta tccacagggt ccacgctctt aagacgaggg cgtcttggga
     3601 catatactct tctcagaatc atagtgagat cttcgggttg gggataaccc gttccagcag
     3661 atagccactc tgcgtcggag gctaggaaga catgaagatc cggtgtgcca aggacgagct
     3721 ctatgaagcc atccagaccg tgcagcgcgc ggtctcggga cgcagcacgc tgcccatcct
     3781 caacaatgtg ctgctcgagg ccgatgatga cacgctctgc ctggtggcct acgacctcga
     3841 gtttggcatc gagtgcaccc tggcggtgaa ggtggaggcc cggggcgggg ccacgatccc
     3901 cgcgcgcatc ctcgcggagg ttgtggggag tatgcctgag gccgagatca ccatcgaggt
     3961 ggatgagcgc caggtggtga ccatcacctg tggtggctcc cggtacgaga tccacggcct
     4021 tccagctgag gagttcccgc gtcttccgct cctgactgaa ggtgcccgcg cgctggtgaa
     4081 ccgcgctcgc ttccggcagc taatccgtag cacctcattc gccacctcgg tggacgagac
     4141 acgccccatc ctcacgggtg ttctcacgca gctgacggca gatacgatca tg
//
LOCUS       DAOSPL010000191         4173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21397_length_4173_cov_3.400437, whole genome shotgun sequence.
ACCESSION   DAOSPL010000191 DAOSPL010000000
VERSION     DAOSPL010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21397_length_4173_cov_3.400437"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PLQ54_04860"
     CDS             <1..800
                     /locus_tag="PLQ54_04860"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOS92619.1"
                     /translation="LSSLTQLAFLDVSTNQLTSLSFASSLSNLMDLIASGNQLTSMAP
                     LVGLTHLTGLDLCSNQITDLSPLASMTQVLELRIADNGITNVAPLSNLAGLQILDIGH
                     NPIGSVSVISSLSALTQLSMAECLLTDVGTISALTNLTALNLEGNRITDILPVTLLTG
                     LRGLDLSGNGLADISTIGALTQLRNLDLSDNLIADLGPLAGLTFLSNVNLANNAIADI
                     SPLVANAGLGANDTVNLVGHRLDLTPNSDDQQDVDALVARGVNVILT"
     gene            911..1537
                     /locus_tag="PLQ54_04865"
     CDS             911..1537
                     /locus_tag="PLQ54_04865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS92620.1"
                     /translation="MEPRTVTTSVGPVVLRPMDRDYIMAADEEDAGVSVEVQCRWREE
                     PWPNPLYETYFQKLIEAYGSCAIMAWSGRTVVGYLPFRPVGTGMPQIPFCMHYYDDRD
                     LAAVEVAQPTPFDSLAPRILQVRCLSVKPSLRRGELDRAAAQYLVEWAREHGWQRIEG
                     WALAEPACAWLPDIRLWEQVGFRRGQRRHINEDNACTQRPGFEFSMDL"
     gene            1550..3613
                     /locus_tag="PLQ54_04870"
     CDS             1550..3613
                     /locus_tag="PLQ54_04870"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOS92621.1"
                     /translation="MGCLATACLAAFILPAGAPSPWDEYRAILWISELPADPGKADLL
                     FRRLREMGCNAASCYPGMDAEAFRRNGVPFYVENLTRCLFIKGDRGWDAAWQGFHDTR
                     DPAYFVRKPCLNDPAVREDDYRRIAEAVGRYGPYQPFAYDIRDEPSVTTSANPFDFCL
                     CERCLSMFREWLQAEYGGLEALNAEWGTAHAAWDSVRPTTTDEAKARVRDGDWNLAAW
                     ADHRAFMDATFADVVGDFAARIVEADPHARAGLEGTQMPHAFGGYDFPSFLPKLDWIE
                     PYDMGSSREIVRSLAPGTPCVSTLFESDENQITRRLWYLLLHGDRGAIVWWSPECIDL
                     EAEDMPLTEKGRAYARPFGELTSDIGRTVIGARPVTDPVAIHYSQASIRADWMIETQQ
                     DGDTWIRRFSSYEADHNQQAAARNAMVKLVEDLGLQAKFVDSARIEAGVLEQEGYRVL
                     LLPRSYALSDAEAQAIGRFVEAGGTAIADGVCGLFDEHCRLRPEGVLDGLFGVTGHAL
                     AFQGDPATEGAAPGGLPLLEPGLRATGGAPAVLADRRTGSGRAVYLNRDVTPYATLRL
                     HPGTAAAAIRKLMAGFLPDDVRPWAQVIVAGTMEPAPACEVHRLQTPEDMLLAVMRNP
                     QFQMSEELRAYGGNEALETPMAVEIRLPAPFAVRDARTGEERGITDRIEVTLSPWEPV
                     IYRLQ"
     gene            3795..>4173
                     /locus_tag="PLQ54_04875"
     CDS             3795..>4173
                     /locus_tag="PLQ54_04875"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HOS92622.1"
                     /translation="MTTARRGVALAALVFLGLGLPPGRAELSPHFDPATMMLPSEVTR
                     GMKAQCRTVFRGTDITEFNIELISVLPKANLGGDLIIFRALDGPLVERGAGILQGMSG
                     SPVYVDGRLLGAVSRTFGGSKEPL"
BASE COUNT          686 a         1351 c         1416 g          720 t
ORIGIN      
        1 cgctgtcgtc gctgactcag ctcgccttcc tggatgtgtc cacgaaccag ctaacgagtc
       61 tcagcttcgc atcctcgctg agcaacctca tggacctgat cgcgtcgggt aaccagctga
      121 ccagcatggc cccgctggtg gggctgaccc acctcacggg actggacctg tgcagcaatc
      181 agatcacgga cctctcgccg ctggcgagca tgacccaagt tctcgaactg cggatcgccg
      241 acaacggtat cacgaacgtg gccccgctct cgaacctggc cgggctgcag attctggaca
      301 tcggccacaa cccgatcggc agcgtgagcg tgatctccag cctgtcggct ctgacccaac
      361 tctcgatggc cgagtgcctg ctcaccgatg tggggaccat atcggcattg accaatctca
      421 cggcgctgaa cctggagggg aaccggatca ccgacatcct gcccgtcacc ttacttacgg
      481 gcctccgagg actggatctg tccggcaacg ggctggcgga catctcgacg atcggcgcac
      541 tcacccagct gaggaatctg gacctgagcg acaacctgat cgcggatctg ggccctctgg
      601 ctgggctcac gttcctgtcc aacgtcaacc tggccaacaa cgccattgcg gacatctcgc
      661 cactggtggc caacgccggg cttggggcca atgacaccgt gaacctcgtg ggacaccgct
      721 tggatctgac acccaactcg gacgatcagc aagacgtgga cgccctggtg gcgcgcggtg
      781 tgaatgtgat tctgacatag ccccagtgaa gtgatgcgcc gatagatcat gcggcctggt
      841 ggcaggccgc ggggcgggca tcgatgagcc gctcggacgc gccgggcagg gcagggaggg
      901 gaggggtgcc gtggagccca ggaccgtgac cacgagtgtg ggtcccgttg tccttcggcc
      961 tatggaccgc gactacatca tggcggcgga cgaggaggac gccggggtgt cggtggaggt
     1021 ccagtgccgg tggcgggagg agccgtggcc taacccactc tatgagacct acttccagaa
     1081 gctcatcgag gcctatggct cgtgcgccat catggcctgg agcggccgta cggtcgtcgg
     1141 atacctccct ttccgccccg tgggcacggg catgccccag atcccgtttt gcatgcacta
     1201 ctacgacgat cgtgacctgg ctgccgttga ggtcgcccaa ccgactccgt ttgactcgtt
     1261 ggcgccccgc atcctccagg tgcggtgcct gagtgttaaa ccctcactgc gccgaggcga
     1321 actggaccgg gcggccgccc agtacctggt cgagtgggcc cgcgaacatg gctggcagcg
     1381 catcgaaggg tgggcgctcg ccgagcccgc ctgtgcgtgg ctgccggaca ttcggctctg
     1441 ggagcaggtc gggttccgtc ggggccagcg gcggcacatc aacgaggaca atgcatgcac
     1501 gcagcggcct ggcttcgagt tcagcatgga cctgtgaggt cgatgtgcca tgggctgtct
     1561 cgcgaccgcg tgcctcgcgg ccttcattct gccggccggc gcgccatcac cctgggatga
     1621 gtaccgcgcg attctgtgga tcagtgagtt acccgccgac ccggggaagg ccgacctgct
     1681 gttccggcgg ttgagggaga tgggctgcaa tgcggcctca tgctacccgg ggatggacgc
     1741 cgaagcgttt cggcgcaacg gggtgccctt ctatgtcgag aacctgaccc gatgcctgtt
     1801 catcaagggt gatcgcgggt gggacgccgc ctggcagggc ttccatgaca cgcgagaccc
     1861 cgcgtacttt gtccggaagc catgcctgaa cgatcccgcg gttcgcgagg acgactatcg
     1921 ccgcatcgcg gaggcggtcg gacgatatgg cccctaccag ccgttcgcct atgacattcg
     1981 cgatgagcca tcggtgacca catcagccaa ccccttcgac ttctgcctct gcgagcgctg
     2041 cctgtcgatg tttcgggaat ggctgcaggc tgagtacggc ggcctcgagg ccctgaatgc
     2101 cgaatggggt accgcccatg cggcgtggga ctcggttcgg cccacaacca cggatgaggc
     2161 gaaggcgcgg gtgcgagacg gggactggaa tctggccgcg tgggccgacc accgggcatt
     2221 catggacgcc accttcgccg atgtcgtggg tgacttcgcg gcccgcatcg tggaggccga
     2281 tcctcacgcc cgggccggcc tcgaagggac ccagatgccc catgccttcg gcgggtacga
     2341 cttcccgagt ttcctgccga agctggattg gatcgagcca tatgacatgg ggagctcccg
     2401 ggagatcgtg cggtcgctgg cgccgggtac tccgtgcgtg tccactctat tcgaatcgga
     2461 cgagaaccag atcactcgcc ggctgtggta cctgcttctc catggcgacc gtggagccat
     2521 cgtctggtgg agccccgagt gcatcgatct cgaggccgag gacatgccgc tcacggagaa
     2581 gggccgagct tacgcccggc ccttcggcga gctcacttca gacatcgggc gaaccgtgat
     2641 cggcgcccgg cccgtcaccg accccgtcgc catccactac tcgcaggcca gcatccgtgc
     2701 tgactggatg atcgagacgc agcaagatgg ggacacgtgg attcggcggt tctcctcgta
     2761 cgaggccgac cacaaccagc aggcggccgc gcggaatgcc atggtgaaac tggtggagga
     2821 cctcggcctg caggccaagt tcgtcgactc ggcgcggatc gaggcgggag tgctcgagca
     2881 ggagggttac cgcgttctgc tactccctcg gtcgtatgcc ctgagcgatg ccgaggccca
     2941 ggccataggg cgtttcgtcg aggccggcgg caccgccatt gcggacgggg tgtgcgggct
     3001 gttcgacgag cactgccgcc tccgtcccga gggcgtcctc gatgggctgt tcggggtcac
     3061 cggacacgcg ttggcgttcc agggcgaccc ggccacggag ggagccgccc ctggcgggct
     3121 ccccctcctc gagccgggtc tgcgggcgac aggcggggca cccgctgtcc tcgcggatcg
     3181 ccgcaccggc agtggacgcg cggtgtacct gaaccgagat gtcaccccct acgcgacgct
     3241 ccggctgcac ccgggcacgg cggccgcggc catccggaag ctcatggccg ggttcctgcc
     3301 cgatgacgtg aggccctggg cgcaagtgat cgtagcgggt accatggagc ccgcgccggc
     3361 gtgcgaggtc catcggctgc aaacgccgga ggatatgctt ctcgccgtca tgcgcaatcc
     3421 gcagttccag atgtccgaag agctgcgagc ctatggtggc aacgaggctc tggagactcc
     3481 gatggcggtg gagatccgcc ttccggcccc gttcgccgtg cgggacgcgc gcacggggga
     3541 ggagcgcggc atcaccgacc ggatcgaggt tacgctctcg ccctgggagc ccgtgatcta
     3601 tcggctgcag tgacccggcc gggaggagag gccatgggcc gggtcgaagc acgatctgtt
     3661 gagagttgag aagcagttgc tcgccgcgga gcgtctggcc ctatgggctg accggcgatt
     3721 gccatcgcgc gtgtggaacg gcaccgcgcc gatgcgccgt tgtgcgagtc cttcatctac
     3781 gaggagagat cccgatgacc acagcccgcc gcggcgtcgc tctggccgcg ctagtcttcc
     3841 tggggttggg cctgccaccc ggacgcgccg agctctcgcc gcacttcgac cccgcgacca
     3901 tgatgctccc gagcgaggtc acgcgaggga tgaaggccca gtgccggacg gtgttccggg
     3961 gcacggacat caccgagttc aacatcgagc tcatcagtgt gctgcccaag gcaaatctgg
     4021 gcggcgacct gatcatcttc cgagcgctgg acggcccgct cgtcgagcgt ggggcgggca
     4081 ttctccaggg catgagcgga agtccggtct atgttgacgg aaggctgctg ggagccgtat
     4141 cgaggacctt tggcggttcg aaggagccgc tgg
//
LOCUS       DAOSPL010000192         4165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21435_length_4165_cov_2.650365, whole genome shotgun sequence.
ACCESSION   DAOSPL010000192 DAOSPL010000000
VERSION     DAOSPL010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21435_length_4165_cov_2.650365"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3637
                     /locus_tag="PLQ54_04880"
     CDS             <1..3637
                     /locus_tag="PLQ54_04880"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOS92623.1"
                     /translation="VYLYDAPAQEVAQDSEEDLQVTSEADAEDEPEGLSDDTEGEAPA
                     AGVPDMVLCCGCLSLFPPSMPRSCGCADAYPTRVVKLRRGRGTRCPICRSRYGAQDVL
                     TPVSLGNSSALTHVSRMLLRELPEESRKLLIFCDSRQDAAHQARFMEGTERHLRLRGA
                     VYRALRHEDAAHDFDWVIESVYADYVEHGWVERARSADAQRRAKDRILGQLLNEFVFS
                     PTARRGLLRLGLVGVTYAGLDDDLESDDLTALCREHEMPVELVRYAVPHLLHTMRGRR
                     AVSQEALTQKLDRGHPLALRLGLVLGRGVGLPVGYQMPGEARRKEPFYDLMPSWSPKG
                     AAAGLQSLWRHLLGEGATRESLAAVTQWLIDRGLVRWVEIGDKSAKAKALQIDYGGVL
                     LSPARTTTQCDVCQRRAVNDVAGAPCTRSGCAGTMRAWQGALAEGNLDATIIAAPAAT
                     PLMPAEHSAAVTDDARREAEEGFMAMPPRPNVLACTPTLELGVNIGDLEAVAMRNIPP
                     SPANYAQRAGRTGRHSRMGLVIGFARETPHDGYFFEKPAEVITGAIPAPRFNLANREA
                     LERHIRSLLLEVARIDFPSDLEPFITEEGQLIAPAVEELLRKLEGARGPAAARAREVF
                     RDTAGGASAELEGTIDRVIDGTAARVEQALTERAKLIEGAAARMAEVGQKVQQLRRDE
                     AEEKGYRALANKLRIDRHYAYLPRVLAEAGVLPGYAFPGDPGSLALGYDPNAVMTGRT
                     QAQREYAPGQVVYARGKRWHVGGLALHRPGARSSAEAQRFSFTRCERCGLAGPAHGAN
                     NCPRCESELPGATQTALDAGAFQAWEQEVEPEAEEERLFGGYDIGVHPQRDGAGRAYS
                     LGDWGLELRFQESVWWINHGSRELSPEGQMGPGSGFILCEACGELHRPPAPAKRKRGS
                     KTRDMAAERHARDCGGADELVAIGHQGNADTLRVVVPGLDSLGPEAPRWAWSFAHAMI
                     QGAAREFVIDPRDIEPVVLVAQSADGLTKGVIELLWADTVTGGSGILSAMAERFPAVA
                     RAALEHLNGHDCPSSCYRCLRTYTNQRVHSLLDWRIVVPQLQALADATLSQAIDLPRQ
                     WGLCQGPEWDEARAEGCESPAELRLLKALRAGGLPEPAKQHEVHSADGVLLTRADFAY
                     PEQRLLIYVDGLAFHSLLRQRVHDSRQTAALQALGWQVMRFLGTEVYRDAASRAAEVA
                     KVVGARR"
     gene            3819..>4165
                     /locus_tag="PLQ54_04885"
     CDS             3819..>4165
                     /locus_tag="PLQ54_04885"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOS92624.1"
                     /translation="MVPAYRGLALVCVVLAVAGLVSAPARATRARLLSVTTDPRGAEV
                     RVDGRFVGYTPLANCHLPDSPTGRYTIRFELPAHRPATRTVELPPGGHAQLAHIEHRL
                     DAQPDLPCLEARVL"
BASE COUNT          624 a         1441 c         1511 g          589 t
ORIGIN      
        1 tgtgtacctg tacgatgcac cggctcagga ggtcgcgcag gacagcgagg aggacctgca
       61 ggtcaccagc gaggccgacg cggaggatga gccagagggg ctgtcggacg acacagaggg
      121 cgaggccccg gcggcaggcg tcccggacat ggtgctgtgc tgcgggtgcc tgtccctatt
      181 ccctccgagc atgccccgca gttgtggctg tgcggacgcg tacccgacgc gcgtcgtgaa
      241 gctgcgccgc gggcggggta cgcgatgccc catctgccgg agccgatacg gcgcccagga
      301 cgtcctgacg cccgtcagcc taggcaactc gtcggccctc acccacgtgt cgcgtatgct
      361 gctgcgggag ctgcccgagg agagccggaa gctgcttatc ttctgcgaca gccggcagga
      421 tgcggcacac caggcgcgct tcatggaagg caccgagcgt cacctgcgcc tccgcggggc
      481 cgtctaccgc gcgctgcgac acgaggatgc cgcgcacgac ttcgactggg tcatcgagag
      541 cgtctacgcg gactacgtcg agcacggctg ggtcgagagg gcgcgctcgg ccgatgccca
      601 gagacgcgcc aaggaccgca tcctcggcca actcctgaac gagttcgtgt tctcgccaac
      661 tgctcggcgc ggccttctgc gcttggggct cgtgggggtc acgtacgcgg gactcgatga
      721 tgacctggag agcgacgacc tgaccgcgtt gtgccgggag cacgagatgc cggtcgaact
      781 cgtgcgctac gccgtccctc acctcctcca cacaatgcgc ggccgacgcg ccgtaagcca
      841 ggaggcgctc acccagaagc tcgaccgcgg ccaccccctg gccctgcggc tgggcctcgt
      901 tctcggccgt ggtgtcgggc tgcccgtggg ctaccagatg ccaggcgagg cgcgccggaa
      961 ggagcccttc tacgacctga tgccgagctg gagccccaag ggcgccgctg ccggcctgca
     1021 gtcgctctgg cggcacctgc tgggcgaggg ggcgacccgg gagtcactgg ctgccgtgac
     1081 gcagtggctc atcgaccggg ggctggtgcg ctgggttgag atcggggaca agagcgccaa
     1141 ggccaaggcc ctacagatcg actatggcgg cgtcctgctc agccctgctc gcacgacgac
     1201 ccagtgcgat gtgtgccagc gtcgggcggt caacgacgta gctggcgcgc cgtgcacccg
     1261 atcgggctgc gccggcacca tgcgcgcgtg gcagggcgcc ctggctgagg ggaacctcga
     1321 cgcgactatc atcgctgccc ctgcggcaac ccccctcatg ccggccgagc actcggcggc
     1381 ggtgaccgac gacgcccgga gggaggccga agaggggttc atggccatgc cgccgcggcc
     1441 caacgtcctg gcgtgcacgc ccacgctcga gctgggcgtg aacatcggtg acctcgaggc
     1501 cgtggccatg cgcaacatcc cgccgagccc ggcgaactac gcgcagcgcg cgggccgtac
     1561 cggccggcac tcgcggatgg ggctggtgat cggctttgcg cgcgagacgc cgcacgacgg
     1621 ctacttcttc gagaagccgg ccgaggtcat caccggcgcg atcccggccc cgcggttcaa
     1681 cctggcgaac cgcgaggcgc tcgagcgcca catccgcagc ctgctactgg aggtcgcgcg
     1741 gatcgacttc ccctcggacc tcgagccgtt catcacggag gagggccagc tcatcgcccc
     1801 tgctgtcgag gagctgctcc gcaagctcga gggcgctcgg ggcccggccg cggcgcgagc
     1861 cagggaggtc ttcagagaca cggcgggcgg agcttcggcg gagctcgaag gaacgatcga
     1921 ccgagtcatc gatggcacgg cggcacgggt ggagcaggcg ctgaccgagc gggccaagct
     1981 catagagggt gcagccgcga ggatggccga ggtgggacag aaggtgcagc agctgaggcg
     2041 cgacgaggcc gaggagaagg gctaccgcgc tctggccaac aagctccgga tcgaccgcca
     2101 ctacgcctac ctaccgcgcg ttctggccga ggccggcgtg ctcccgggct acgcgttccc
     2161 gggcgacccc ggctcgctgg ccttgggcta cgacccgaac gccgtaatga cggggcgcac
     2221 ccaggcgcag cgcgagtacg cgcccggaca ggtagtctac gcccgtggca agcgttggca
     2281 tgtcggtggc ctggccctcc accgcccggg cgcccgctcg tcggctgagg cgcagcggtt
     2341 cagcttcacg cggtgcgagc ggtgcggact ggcaggccca gcccacggcg ccaacaactg
     2401 ccctcgatgc gagtccgagc tccccggcgc cacgcagacc gccctcgacg ccggcgcgtt
     2461 ccaggcatgg gagcaggagg tcgagccaga ggccgaggag gagcggctgt tcggcggcta
     2521 cgacattggg gttcaccccc agcgggatgg ggccggccgc gcctactcgc tcggcgactg
     2581 gggcctcgag ttgcgcttcc aggagtcggt gtggtggatc aaccacgggt cgcgcgagct
     2641 gagccctgag ggccagatgg ggcccggctc tgggttcatc ctgtgtgagg cctgcggcga
     2701 gctgcaccgg ccccccgcgc cagccaagcg caagcggggg agcaagaccc gcgacatggc
     2761 cgcagagcgc cacgcaagag actgcggcgg ggccgatgag ctggtggcca tcgggcacca
     2821 gggcaatgcc gacacgcttc gggtggtcgt gcccggcctg gactcgctcg ggcccgaggc
     2881 gcccaggtgg gcgtggagct tcgcgcatgc catgatccag ggcgctgccc gggagttcgt
     2941 catcgacccg cgcgacatcg agccggtggt gctggtcgcc cagtccgccg acggcctgac
     3001 caaaggggtc atcgagctgc tgtgggccga cacggtgacg ggcgggtcag ggatcctctc
     3061 ggccatggcc gagcgcttcc cggccgtcgc ccgcgccgcc cttgagcacc tgaacgggca
     3121 cgactgcccc agctcgtgct accggtgtct gaggacgtac acgaaccagc gcgtgcactc
     3181 gctgctcgac tggcgcatcg tggtgccgca actgcaggcc ctcgcggacg ccacgctttc
     3241 tcaggcgatc gacctgccca ggcaatgggg gctctgccag gggccggaat gggatgaggc
     3301 ccgcgccgag ggctgcgaat cgccggccga gctccgcctg ctgaaggccc tgcgggctgg
     3361 tgggcttccg gagcccgcga agcagcacga ggtgcacagc gccgatggcg tgctcctcac
     3421 ccgcgccgac ttcgcgtacc cggagcagcg gctgctcatc tacgtcgacg gcctggcttt
     3481 ccactcgttg ctccgtcagc gcgtccacga ctcacggcag actgcggcgc tccaggccct
     3541 gggctggcaa gtcatgcgct tcctcggcac agaggtctac cgcgatgctg cgtcacgcgc
     3601 ggccgaggtg gcgaaggtgg tgggcgcgag gcgctgacag gcggcgagac acaagcgctt
     3661 gggagagtgg ggcgcccgta cggccgaggc ccctgtccga acgtgacctc tgggccagcg
     3721 cagggccgcc gttcgaccga gccgaaggac cacctgagcg gcgacggcat cgcggcggct
     3781 tggttggggc ccagcatcgg atgtcggagg tgtgagcggt ggttcccgca taccgagggc
     3841 ttgcgctggt gtgtgtggtg cttgccgtgg ccgggctggt gtctgccccg gcgcgggcta
     3901 cccgggcgcg cctgctgagt gtcaccaccg atcctcgggg ggccgaagtc agggtcgatg
     3961 ggcgcttcgt cgggtacacc ccgctggcta actgccacct ccctgacagc cctaccgggc
     4021 gctacaccat ccgcttcgag ctgccggccc atcgccccgc aacaaggacc gtcgagctcc
     4081 cgcctggcgg ccatgcccag cttgctcata tcgagcaccg gctcgatgcg cagccggatc
     4141 tgccctgcct cgaggcgcga gtact
//
LOCUS       DAOSPL010000193         4161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21457_length_4161_cov_3.297126, whole genome shotgun sequence.
ACCESSION   DAOSPL010000193 DAOSPL010000000
VERSION     DAOSPL010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21457_length_4161_cov_3.297126"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="PLQ54_04890"
     CDS             <1..1260
                     /locus_tag="PLQ54_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOS92625.1"
                     /translation="CTGSQGDPFRGLAAAEAAAEPGDLFRVGAGRYEGTFEVGHSGEP
                     GRPIIWRGEGEAVLDGQGQATERPGRAISASDVHDVWFEGLDVRNADYGIVAHRSARI
                     VVRGCHISGVEYGLTCTNNDDGSVQGFFICDNTIEGPSVWPRSKGIEDARGIQVTGTG
                     HVVCYNRITGFADAIDTFPSTRCSSIDLHNNDISVMTDDGIEMDYSERNTRCFENRLT
                     NVFQGISFQPVLGGPVYAFRNALYNVAVEPFKLHNSPSGALMLHNTCVKCGMPLELWT
                     NEPVRNCVSRNNLFIGTTAAYAYETTALMQGCDFDYDGFGGGPWERFLKWNDARFATL
                     DDARRKAPVYTHAVLVDPATAFASGAQAPESADVRFDVPPDLRLAADSAAVDAGAPIG
                     FCGLVSGVAPDLGAYELGEPLPHYGPR"
     assembly_gap    1374..1473
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1671..3068)
                     /locus_tag="PLQ54_04895"
     CDS             complement(1671..3068)
                     /locus_tag="PLQ54_04895"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="catalyzes the formation of D-fructose 6-phosphate
                     from D-glucose 6-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOS92626.1"
                     /translation="MVTVDISNALAERIGPEYGLSRDEITGLMGSHPASVITARKRGA
                     ERPAFTYSAEQTEGQLDAILAECSAFMGQFDRGLSDVIILGIGGSALGARALKAALLH
                     PFDDMIPVPQRDHPRLHVLDNVDPAQTAALLDVLDPSTTGLIAISKAGGTAETVAGFA
                     VARQWLSGAQRDWRKQSLIITNEPSSDGKTTLLWDLAGSEGVRRVGMPIPLGGRYSVL
                     SPVGLVPAALVGMDVKALVAGAARMYARCESTDPWENPAYLLAAVCYLYMRRPNHPRN
                     ICVLMPYSSALSYMADWFVQLWAESLGKARNLRGDLVNAGQTPVPAVGATDQHSQLQL
                     FQEGPHDKVILFIEVGDYGGDLEIPPALPGEPTYLAGKTLGQLLSAEKRGTEFALTDS
                     QRPNVTIRVPAITADTVGELFFLFWMQTAITAELMEINAFDQPGVEASKNATRALMGA
                     PGAENDELRRRMGVE"
     gene            complement(3088..3837)
                     /gene="lipB"
                     /locus_tag="PLQ54_04900"
     CDS             complement(3088..3837)
                     /gene="lipB"
                     /locus_tag="PLQ54_04900"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006634349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOS92627.1"
                     /translation="MADSVARAPCLVANLGLVPYDQALALQQRIHQARVGGAGPDVLL
                     LLEHPPTITLGRSARAEHLLSAPELLARRGIALHAVGRGGDVTYHGPGQLVGYPILDV
                     STYRGALSRYLRDLEQMLIDVLASFGVRGERAEGLTGVFVGGAKIAAIGVQVKRWVSL
                     HGFAMNVSTDLSAFGHIVPCGIRDRAVTSLAEHVHPAPCMGEVARTVVSSFATVFGRE
                     MTWGAGASWPQHPLADGRVTEGRPPTCSDRA"
     gene            3971..>4161
                     /locus_tag="PLQ54_04905"
     CDS             3971..>4161
                     /locus_tag="PLQ54_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018770048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOS92628.1"
                     /translation="MRILVIGGTGHIGSYLVPRLVLGGHEVTVVARKPSPQYTDSRLA
                     WGAVEWIVADRGAEEAAGT"
BASE COUNT          649 a         1384 c         1415 g          613 t
ORIGIN      
        1 tgcaccggca gccagggcga cccgttccgc ggactcgcgg cggccgaggc cgccgcggag
       61 cccggcgatc tgttccgggt gggagccggg cggtacgagg gcaccttcga ggtcggtcac
      121 agtggcgaac cgggcaggcc gatcatctgg cgcggcgagg gcgaggccgt gctggacggc
      181 cagggacagg ccaccgaacg ccccggccgg gccatcagtg cgtccgacgt gcacgatgtc
      241 tggttcgagg gtctggacgt ccgtaacgcc gactacggca tcgtggccca ccgctcggca
      301 cgcattgtgg tccgcgggtg tcacatcagc ggcgtcgagt acggtctgac ctgcaccaac
      361 aacgatgacg ggtccgtgca gggcttcttc atctgcgaca acacgatcga gggcccgtcc
      421 gtctggcccc gctccaaggg gatcgaggat gcccgaggca tacaggtcac cggcaccggc
      481 cacgtcgtct gctacaaccg catcaccggt ttcgccgatg ccatcgacac gttcccttcg
      541 acccgctgct catccatcga ccttcacaac aacgacatca gcgtgatgac cgatgacggc
      601 atcgagatgg actactcgga acggaacacg cgctgcttcg agaaccggct gacaaacgta
      661 ttccagggca tctccttcca gcccgtgctc ggcggcccgg tctacgcctt ccggaacgcg
      721 ctgtacaacg tggccgtcga gccattcaag ctccacaaca gcccgtccgg agccctgatg
      781 ctccacaaca cgtgcgtgaa gtgtggcatg ccgctcgagc tctggaccaa cgagccggtg
      841 cggaactgcg tgtcgcggaa caacctgttc atcggcacga ccgccgccta cgcctacgag
      901 acgacggctc taatgcaggg gtgtgacttc gactacgacg ggttcggtgg cggcccatgg
      961 gagcggttcc tcaagtggaa cgatgcccgt tttgcgacgc tcgacgacgc cagacggaag
     1021 gcacccgtct acacccacgc ggtactcgtc gatcccgcga cggctttcgc cagtggggcg
     1081 caggcccccg agagcgccga tgtccgcttc gatgtcccgc cggacctgcg gctggccgcg
     1141 gactcggcgg ccgtcgatgc cggtgccccc atcggcttct gcggcctggt ctccggcgtc
     1201 gcgccggacc tgggtgccta cgagctcggc gagccgctcc cgcactacgg gccgaggtag
     1261 gggcggctgc cattgacctg gtggcctcgg cgtacgcgca ggccggtctc ggagcgcgga
     1321 gcgcctgctc cgcgtgggca gggcaccgca acggcgcggg gcgggagccc cgcnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcggggc gggagccccg cgggcacgaa
     1501 cgaccggatc cagtgccggc ggccgtccgc gccccggcgt gagcaccagc cggtctcgga
     1561 gcgcggagcg cctgctccgc gtgggcaggg cacggtcgaa ccgcggacgc ggccgtccgc
     1621 gccccggcag gctgaagcct gggctcccgt tcggcacgcg gctgagcccg ctactccacg
     1681 cccatccttc ggcgcagctc gtcgttctcg gcgcccggcg cgcccatgag tgcacgggtg
     1741 gcgttcttgc tggcctcgac tcccggctgg tcgaaggcgt tgatctccat cagctcagcc
     1801 gtgatcgcgg tctgcatcca gaacaggaag aacagctcgc cgacggtgtc ggccgtgatc
     1861 gcggggacgc ggatggtcac gttgggccgc tgggaatcgg tgagcgcgaa ctcggtgcct
     1921 cgcttctcgg ccgagaggag ctggcccagg gtcttcccgg ccaggtaggt cggctcgccc
     1981 ggcagtgccg gcgggatctc gaggtccccc ccgtagtcgc ccacctcgat gaacaggatg
     2041 actttgtcat gaggcccttc ctggaagagc tgaagctgcg agtgctggtc ggtggcgccg
     2101 acggctggca cgggcgtctg accggcgttc accaggtcgc cgcggaggtt cctggccttg
     2161 cccaggctct cggcccagag ctggacgaac cagtcggcca tgtagctgag cgccgatgag
     2221 tagggcatga gcacgcagat gttgcgcgga tggttggggc ggcgcatgta gaggtagcac
     2281 accgcggcga gcaggtaggc gggattctcc cagggatcgg tgctctcgca ccgggcgtac
     2341 atgcgggcgg ctccagcgac gagcgccttc acatccatcc caaccagagc ggcggggacc
     2401 aaccccacgg gcgacaggac cgagtaccgc ccaccgagcg gaatgggcat ccccacacgc
     2461 cgcactccct cgcttcccgc caggtcccac aacagcgtgg tcttgccgtc agatgaaggc
     2521 tcattggtga tgatgaggct ctgcttcctc cagtcccgtt gcgctccaga tagccactga
     2581 cgtgccacgg cgaaccccgc cacggtctcg gcggtgccac cggccttact gatggcaatc
     2641 aggccggtgg tcgacgggtc gagcacgtcg agcagcgcgg cagtctgtgc cggatcgacg
     2701 ttgtcgagca catgaagcct cgggtggtcg cgctgcggga cggggatcat atcatcaaag
     2761 gggtggagca gcgcggcctt gagcgctcgc gctccgagcg ccgacccgcc gattcccagg
     2821 atgatcacat cgctcagtcc gcgatcgaac tgccccatga acgcgctgca ctcggcgagg
     2881 atggcgtcga gctgtccctc ggtctgctcg gccgagtagg tgaatgccgg acgctcggcc
     2941 ccgcgcttgc gcgcggtgat gacggacgcc gggtgggatc ccatgagtcc cgtgatctca
     3001 tcgcgggata ggccgtactc gggcccgatg cgctcggcaa gggcgttgct gatatccacg
     3061 gtcaccatgg gtgaccacca ccttccgtca tgcgcgatca ctgcaggtcg gcggccggcc
     3121 ctcggtgacc cgcccgtcgg cgagggggtg ctgcggccac gaggcgcccg ctccccacgt
     3181 catttcgcgc ccgaacactg tagcaaacga cgagaccact gtgcgagcca cctcgcccat
     3241 acacggtgcc gggtggacat gctcggccaa cgaggtgacc gcccggtcgc ggatgccgca
     3301 gggcacgatg tggccgaaag ccgacaggtc ggtcgagacg ttcatcgcga acccgtgtaa
     3361 ggatacccac cgcttcacct gcacgccgat ggcggcgatc ttggcgccgc cgacgaagac
     3421 gcccgtgagc ccctccgccc tctcgccgcg cacaccaaag gaggccagga cgtcgatgag
     3481 catctgctcg aggtcgcgca ggtaccggct cagcgcgccg cggtaggtcg agacatcgag
     3541 gatcgggtag ccgacaagtt ggcccggacc gtggtaggtg acatcgcccc cgcggccgac
     3601 cgcgtggagc gcgatgcccc tgcgcgccag cagctcgggg gcagaaagca gatgctcggc
     3661 gcgcgcgctg cgcccgagcg tgatggtcgg tgggtgctcg agcagcagca agacgtcggg
     3721 accggctcca ccgacccgcg cctggtggat ccgctgctgc agcgcgagcg cctggtcgta
     3781 gggcacgagg ccaaggttgg cgaccaggca tggtgctcga gcaacggaat cggccatggg
     3841 tgcacccctc gtgcctcgcg caggcttcct ggaggatggt agcacagcga acggcgacga
     3901 ggtcgtaagg cccctgctac ggaaccgcat cacggcaggg ccagcgcgga gctggaaggg
     3961 gccatggacg atgcggatcc ttgtcatcgg cggcaccgga cacatcggga gctacctggt
     4021 gcctcggctc gtgctcggcg gccacgaggt gaccgtggtc gcgcggaagc catctccgca
     4081 gtacaccgac tcgcggctgg cgtggggagc cgtcgagtgg atcgtcgccg accgcggggc
     4141 cgaggaggcc gccggcacgt g
//
LOCUS       DAOSPL010000194         4156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21508_length_4156_cov_2.986589, whole genome shotgun sequence.
ACCESSION   DAOSPL010000194 DAOSPL010000000
VERSION     DAOSPL010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21508_length_4156_cov_2.986589"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PLQ54_04910"
     CDS             complement(<1..656)
                     /locus_tag="PLQ54_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92629.1"
                     /translation="MSPRERMVAAYRGLPVDGVPVAPEFWYYIPARLLGVDMITFERQ
                     VPLWEAHQRTFRHYGTEGWSVAGGSIPSPGVRSREDWVDIGGGRFESRVVTETPHGVL
                     RSRTLFDRAEPSSAIERPIKDFVTDWPAYEAASLGAVEEADWSGVRRALAAVGDDYLL
                     EVGVGVPFFDYIALGREGGPTRALFDLADHGPFLQSLHERYLAHMRRLARAACEQTAA
                     "
     gene            748..1812
                     /locus_tag="PLQ54_04915"
     CDS             748..1812
                     /locus_tag="PLQ54_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS92630.1"
                     /translation="MKRLTMRERLLAVLRGHELDEVPFAVYDGTEGTRQDIWDSVGRD
                     CVGLLGWTSAHTAAAPHCHWETEDILVDGRRGLRTVLHTPAGSLVEEKILQPDLGVLA
                     AYRHFVRVPRDYEVLLAYLRDVIVSPSPQTCAATVASLGDDGLPHLAVDRTPFQQLWI
                     QWVCLEDLCLHLVDCPEIVRDCMDAMGALLRREFEAACRASAPYVVIPDNITAPAIGP
                     RLFREYCIPYYRELAAMLGEHDMRLFVHMDGDLRPLWADIADSGVGGIDSLSPPPDND
                     TSVAAALSQWPDMRLFVNFPSSVHLAPPEQVYAQTAQMLAEGKASGRLEIQISENLPP
                     GRWRVSYPAIVAAIRDLGQY"
     gene            complement(2169..3362)
                     /locus_tag="PLQ54_04920"
     CDS             complement(2169..3362)
                     /locus_tag="PLQ54_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS92631.1"
                     /translation="MSKVTFFNIPCHGHMNPALALAQELVRRGECVRFYCTREFEPRI
                     TATGAQFRPYPIDDSYVRPTGEVFEGAARLMEACTAGYGPLCQDLRESPPDYIIHDSV
                     CVWGKILAHARGIPAVCSVTQLAFSLRVLLTSVPFMSSLAKTVPGGTRWLMRYLRARR
                     RLWAEHRVAPSGILDVFTNHEPLNLVYTSRLVQPQPERFDSSYAFVGPCLGSRGSAHD
                     FPWEQLESRRVICISMGTIDTDLPRFYESCVEAFAQWDGCVVMAVGSEEQVARLRAPA
                     HFVIRASIPQLEVLQRAAVLISHGGINSIQEAFWHNVPVVLVPGTAERILMSRQVVRL
                     GAGILLSPHRATPARLAEAVHHVVTDPGIQANVAQVGASLREAGGVIRAADLIEEYKA
                     AHGVA"
     gene            3464..4087
                     /locus_tag="PLQ54_04925"
     CDS             3464..4087
                     /locus_tag="PLQ54_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92632.1"
                     /translation="MGSLIEKLFDWAYHVEPLAENSDHLNFRVCFQTYRGRSIDLPPG
                     IRVERGARVVELHQKNEMLARLHDDHPSPRAAALAYSRLMGESLAALARAWREDPRFQ
                     GVVAAYGVNLFADVAVRGGFTARPIRPEWRRKLLSWWIRRIVASHHPAGRARVIKNGV
                     PLMAQEVWISREVCQQLYGEATEQSDALDAARKSRRLQTALGQNRED"
BASE COUNT          691 a         1457 c         1362 g          646 t
ORIGIN      
        1 tccgccgccg tctgctcgca cgcggcccgt gccagccggc gcatgtgggc caggtagcgc
       61 tcgtggagcg actggagaaa cgggccatgg tccgccaggt cgaagagcgc ccgggtgggg
      121 ccgccctcgc gcccgagggc aatgtagtcg aaaaagggca cgcccacgcc cacctcgagc
      181 aggtagtcgt cgcccactgc cgccagggcg cgtctgaccc cggaccagtc ggcctcctcc
      241 acggcaccca ggcttgccgc ctcgtaggcc ggccagtcag tcacaaagtc cttaataggt
      301 cgctcaatcg cggatgacgg ctcggcccgg tcgaagaggg tacggcttcg gagcactccg
      361 tgcggagtct ccgtgacaac gcgcgattcg aaccggccgc cgccgatgtc gacccagtcc
      421 tcgcggctcc gcacgcccgg cgacgggatg ctgccccccg cgacagacca gccctcggtg
      481 ccgtagtggc ggaaggtccg ttggtgggcc tcccacagcg gcacctgccg ctcgaacgtg
      541 atcatgtcca ccccgaggag ccgcgccggg atgtagtacc agaactcagg ggccaccggc
      601 acgccatcga cgggaaggcc gcggtaggcg gcaaccatgc gctcgcgggg agacaaggca
      661 ttccctcctg ctccggaagc gacacaggca tcatagaccg acgtgccgaa ccgtgtcgcg
      721 atctacggcg cgggacggtg cgatgccatg aagcgtctga ccatgcgcga acgcctcctg
      781 gcggtgttac gcggccacga gctcgatgag gtgcccttcg ccgtctacga cgggacggaa
      841 ggcacccggc aagacatctg ggactcagtg ggccgcgact gcgtcgggct gttgggctgg
      901 accagtgccc acacggcggc cgcgccccac tgccactggg agaccgagga catccttgtc
      961 gatggccgtc ggggcctgcg gaccgtcctc cacacgccgg cgggctcgct cgtcgaagag
     1021 aagatcctcc agcccgatct cggcgttctt gcggcctacc gccatttcgt gcgtgtcccg
     1081 cgggactacg aggtccttct cgcctacctc cgagacgtga tcgtctcgcc cagcccgcag
     1141 acatgcgccg cgacggtagc cagcctgggc gatgacggtc tccctcacct cgccgtcgac
     1201 cgcacgccct tccagcagct ctggatccag tgggtgtgtc ttgaggacct gtgcctgcac
     1261 ctcgtcgact gcccggagat cgtgcgcgat tgcatggacg ccatgggggc attgcttcgc
     1321 cgcgagttcg aggccgcttg ccgagccagt gcgccctatg tggtcatccc cgacaacatc
     1381 accgcgcccg ccatcgggcc gcgcctcttc cgcgagtact gcatccccta ctaccgtgag
     1441 ctggccgcca tgctgggtga acacgacatg cggctcttcg tgcacatgga tggcgacttg
     1501 cgccccctgt gggccgacat cgcagactcc ggcgtcggtg ggatcgactc cctgtcgccg
     1561 ccgcccgata atgacacatc ggtcgccgcc gccctgtccc agtggcccga catgcggctg
     1621 ttcgtgaact tcccctcctc ggtccacctc gcaccgcccg agcaggtcta tgcccagacc
     1681 gcccagatgc tcgccgaggg gaaagcgtcc ggtcgcctcg agatccagat ctccgagaac
     1741 ctgccgcccg gccgctggcg tgtcagctac ccggccattg tggccgccat tcgggatttg
     1801 ggccagtact gaaggccgag gcagcccggc cggtcaatga cccccacagt acgccgaacg
     1861 ttccacagcc cgtcttgcgg aggggcctga taggggggaa cagccgtagg ctggtgggac
     1921 cctgatcggc ccggcgctcg acggccctcg acgggccaga gagcgccgtc gtagagcact
     1981 gacggtcttg gtcggtcggc ctggcagccg acgcgggccc attggggccc ctccgccaga
     2041 tccgaacgtg ccgtctcggc gctcctgcgg cgtcatctcg ggcctaactg agcgccagtg
     2101 gggcagagcc ccgcagttgc ccgctgtcgg cggcgtggcg gagccccatg gttgcctcgg
     2161 tcctgaccct acgctacccc gtgcgccgct ttgtactcct cgatgagatc ggccgcccgg
     2221 atcacaccgc cggcctcccg cagcgaggcg cccacctgcg ccacgttcgc ctggatgccc
     2281 gggtccgtta cgacgtggtg caccgcctcg gccagccgcg cgggcgtcgc tcggtgaggg
     2341 ctgagtagta tgccggctcc gagccgcacc acttggcggg acatgaggat ccgctctgcc
     2401 gtgccgggga ccagcaccac ggggacgttg tgccagaacg cctcctgaat gctgttgatc
     2461 ccgccatgtg agatcagcac ggccgcgcgc tgcagcacct cgagctgtgg gatggaagcg
     2521 cggatgacga agtgggcggg ggcgcggagc cgcgccactt gctcctcgga ccccacggcc
     2581 atgacgacgc acccatccca ttgcgcaaac gcctcaacac atgactcgta gaagcgcgga
     2641 agatcggtgt cgatcgtgcc catgctgatg cagatcaccc ggcggctctc cagctgctcc
     2701 cacgggaagt catgggcact gcctcgggag ccgaggcacg ggccgacgaa ggcatagctg
     2761 ctgtcgaacc gctcgggttg gggctgcacc agccgagacg tgtagaccag attcagcggc
     2821 tcgtggttgg tgaacacatc gagaatcccg ctcggcgcga cacggtgttc ggcccagagg
     2881 cgccggcggg cacgcaggta gcgcatgagc caccgcgttc cacccgggac cgtcttcgcc
     2941 aatgacgaca tgaacggtac cgatgtgagc aggacgcgca ggctgaaggc cagctgcgtc
     3001 accgagcaca cggccgggat gccgcgggcg tgcgccagga tcttccccca gacgcacacg
     3061 gagtcgtgga tgatgtagtc gggaggcgac tcccgcaggt cctggcagag cggaccgtag
     3121 cccgccgtgc acgcttccat gagcctcgct gcaccctcga acacctcgcc ggtggggcgg
     3181 acatacgagt cgtcgatggg atagggccgg aactgcgctc ccgtggcggt gatccggggc
     3241 tcgaactcgc gcgtgcagta gaaacggaca cactccccgc gccgaaccag ctcctgtgcc
     3301 agagctagcg ccgggttcat gtgtccatgg cacggtatgt tgaagaaggt aaccttcgac
     3361 acggatggcc tcgcctcttg gtgccgaagg gcgacacctg tcggctgggg cattatactg
     3421 ccccaccagc gggccgccaa agccgcgagg gggacgatcg cgggtgggat cgctcatcga
     3481 gaagctgttc gactgggcct atcacgtcga gccccttgcg gagaactcgg accatctcaa
     3541 cttccgggtc tgcttccaga cctaccgggg ccggtcgata gacctaccgc cgggcatccg
     3601 ggtcgagcga ggcgcccgcg tggtcgagct gcaccagaag aacgagatgc tcgcacgcct
     3661 gcacgacgac caccccagcc cccgtgctgc cgcactggcg tacagcagac tcatgggcga
     3721 gtccctcgcc gcgttggcac gggcttggcg tgaggacccc agattccagg gcgtcgtggc
     3781 cgcctacggc gtcaaccttt tcgccgacgt agctgtccgc ggcgggttca cggcccgccc
     3841 gatccgcccc gaatggcgca gaaagctgct cagctggtgg atccgtcgca tcgtcgcatc
     3901 acaccacccc gctggcagag cacgggtcat caagaatggc gtgccgctga tggctcagga
     3961 ggtctggatc tcgcgcgagg tctgccaaca gctatatgga gaggcaaccg agcagtcaga
     4021 cgccctcgac gcagcgcgaa agagtcgtcg cctccagacc gcgctcggcc aaaacaggga
     4081 ggactagctc caggcactgc gccacacgct caggcgtgtc gtcgaaccct ccggcatcat
     4141 gaaggcacac gatgtc
//
LOCUS       DAOSPL010000195         4153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21536_length_4153_cov_2.892631, whole genome shotgun sequence.
ACCESSION   DAOSPL010000195 DAOSPL010000000
VERSION     DAOSPL010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21536_length_4153_cov_2.892631"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PLQ54_04930"
     CDS             <1..887
                     /locus_tag="PLQ54_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOS92633.1"
                     /translation="LMDWHHPDGARCARNEKARRRFVDYIHGQIRELLTNYGKIDILW
                     YDVNWPLSPEGWESERMNQMALELQPDIIINNRNGLPGDFGTPEQHITPEAAGRMWEA
                     CMTFNTSWGYTPIDTDYKSARHVVEMLRQVAAGGGNLLLNIGPDPLGNVPAPCPSVLR
                     QVGAWLQQYGPSVYEATDPFQGEWLITGAFTRKGDTLYFHCNRWPGSELAIGGLVCEI
                     TSARLMGGPKVKFRQVKDRLVLSGLPEKAPNPLTTVIELGFKGEPRQVLGAGHVVIDK
                     DPWAAPTRKPAAKKTKAK"
     gene            complement(1042..2724)
                     /locus_tag="PLQ54_04935"
     CDS             complement(1042..2724)
                     /locus_tag="PLQ54_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92634.1"
                     /translation="MVIAPTAVEDQRQQRCAYLRTAADSSCAAASLGGSVRPATKLPG
                     EVIGMGAPYGIPLLAAAALSVGMAASATVVELGLGPPGRKVLSAAHFGANAEFFRPDL
                     YFGTLPADDPRSTRAAFVEALHRSGIRSLRFPAGNPSYYYLAESAPLTMELAHATGHW
                     EYVDSSPGSAGFVSLESLCSLAREAGIGLIYELPVLFHLDGATTPRATIRSSLSDVAG
                     NYDRDRIEDCVAYGCGVIRRLRALGAPVLAWELGNEEFALCEAGDYRRLCEAYIPAIR
                     RLDAKTPIVAVGMGAFGSALAGVSGVDALNAHYPFGNWPGPPSADRRGDAAAFAGGDL
                     CMDRWLGSPAGWRRSATPWAVSETMVMHHEFWDPSAVVTTQAHALTYARNWMTLLEHP
                     GCTMAVFHDLATPYFGMMRFNVGYDAVAGQFGWLAADALPGPWTRTFPMEYVLSPTCL
                     ANRMLSTLEGARVTPVGLRPDVPEARFTAGVLPDGTRRLVGVNWSAEPLGLCLRVRGK
                     VRAEVLTATTLAAKLPEEFGVSALPVEPSANGGWVLTVPGESVVWGEVAERG"
     gene            2723..3154
                     /locus_tag="PLQ54_04940"
     CDS             2723..3154
                     /locus_tag="PLQ54_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92635.1"
                     /translation="MAFTVRKIKIRRAKVKNQAGALAQLLAPVAEAGLDLETVVAYAQ
                     KKTGPADVYCSLVDTGKKAGAAADACGFAVDDASPALLVEGDNGPGVLRKVTQAVADA
                     GVSMRVCIALPVGCKFAALLGFDDPADADKAAKKIRAAGRA"
     gene            3368..>4153
                     /locus_tag="PLQ54_04945"
     CDS             3368..>4153
                     /locus_tag="PLQ54_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS92636.1"
                     /translation="MSTDLANFRATVEHRRPERILYHADFVEDLRRRVVDYIGTEDIA
                     GHFGFFSPVGLGPQRPEGLPPLDYTRYWEGEDLPEGTQIDGNGVAMVPSGMYHFWGYI
                     SPLRNAQSLEEIECYPMDDMRDWDWSHLDAIVADAHAQGRVVSAFPGHMYETAWQIRG
                     LEQFLVDVIERPAWAECLLERIAEQNQIRARAYARAGADLIQCGDDVASQVALIFSPT
                     VWRKMIHSRWARIWAEIKSINPQCIIWYHSDGNIIDIVPDMGEN"
BASE COUNT          737 a         1460 c         1355 g          601 t
ORIGIN      
        1 cgctcatgga ctggcaccat cccgatggcg cccgctgtgc ccgcaatgag aaggcccgcc
       61 gccgattcgt cgactacatc catgggcaga tccgcgaact cctcaccaac tacggcaaga
      121 tcgacatcct ctggtacgac gtgaactggc ctctgagccc cgaaggctgg gagtcggagc
      181 gcatgaacca gatggcgctc gaactgcagc ccgacatcat tatcaacaac cgcaatgggc
      241 tccccggcga cttcggcaca cccgagcagc acatcacccc ggaggcggcc ggccggatgt
      301 gggaagcctg catgaccttc aacacgtcgt ggggctacac gcccatcgac accgactaca
      361 agtcggcccg gcacgtcgtc gaaatgctgc gccaggtcgc ggccggcggc gggaatctct
      421 tgctcaacat cggccccgat cccctcggca atgtgcccgc gccctgccca tcggtgctcc
      481 gccaggtcgg cgcctggctt cagcagtacg ggccgtcggt ctatgaggcc acggatccct
      541 tccagggcga gtggctgatc accggcgcgt tcacgcgaaa gggcgacacc ctctacttcc
      601 actgtaaccg ctggccagga agcgagctcg cgatcggcgg cctggtgtgc gagatcacct
      661 cagcgcgcct catgggcggc ccgaaggtga agttccgcca ggtcaaggac cggctcgtgc
      721 tgtccggcct gcccgagaag gcgccaaacc cgctgaccac cgtcatcgag ctcggcttca
      781 agggcgagcc gcgacaggtc ctcggcgccg gacatgtagt catcgacaag gacccgtggg
      841 ccgcgcccac caggaagccg gccgcgaaga agaccaaggc aaagtagtca gagccgaggc
      901 ctggtagtgc cgttccccct ccccgcccgc ggtgagggcc ggggaggggg cttcgctgct
      961 gtcgtcctgc gcgaagtgag aggtcctctc gccagcgggg ccctcgaccg ttctgtcgcc
     1021 gacctcggca ggacacgccc ctcagccccg ctccgcaacc tctccccata ccaccgactc
     1081 acccggcacc gtgagcaccc accctccatt cgccgacggc tccactggca gcgccgagac
     1141 cccgaattcc tctggcagct tggccgccag cgtggtggcc gtcagcactt cggctcgcac
     1201 tttcccccgc acgcgaaggc acaggcctag cggctcggcc gaccagttca cgccgaccaa
     1261 ccggcgggtg ccatcgggta gcacgccggc ggtgaaccgc gcctctggta cgtccggcct
     1321 caacccgacg ggcgtcactc tcgccccttc cagcgtcgac agcatccggt tggccaggca
     1381 cgtcggtgag agcacgtact ccatggggaa tgtgcgggtc cacgggccgg gcagggcgtc
     1441 cgccgcgagc caaccgaact gccccgcgac ggcgtcgtac cccacgttga agcgcatcat
     1501 cccgaagtag ggcgtcgcca gatcgtggaa cacggccatc gtgcagcccg gatgctcgag
     1561 caacgtcatc cagttgcggg cgtaggtcag cgcgtgggcc tgggtggtca ccaccgcgct
     1621 cggatcccag aactcatggt gcatcaccat ggtctccgag acggcccacg gcgtggccga
     1681 ccgccgccac ccggcaggag aacccagcca ccggtccatg caaagatccc cgccggcgaa
     1741 ggcggccgcg tcaccccgcc ggtccgcact gggcggcccg ggccagttgc cgaaggggta
     1801 gtgggcattc agggcgtcca cgcccgagac gccagccaaa gcggatccga acgcccccat
     1861 acccacggcc acgatgggcg tcttcgcgtc gagccgccgg attgcgggaa tgtaggcctc
     1921 acacagccta cggtagtccc cggcctcgca cagcgcgaac tcctcgttcc cgagctccca
     1981 ggcaaggacc ggagcgccaa gcgcacgcaa ccggcgaatc acaccacagc catacgccac
     2041 gcaatcctcg atgcggtcac ggtcgtagtt gcccgccacg tcgctcaggc tcgaccggat
     2101 agtggccctc ggcgttgttg ccccgtcgag gtggaatagc acgggcaact catagatcag
     2161 gccgatgcca gcctcccggg ccagactgca cagactctct aggctgacga agccagcgga
     2221 gcccggactg ctatcgacgt actcccagtg acctgtcgcg tgggcgagct ccatggtcag
     2281 tggtgcgctc tcggccaggt agtagtagga ggggttcccg gccgggaagc gcagcgagcg
     2341 gatcccgctc cggtgcaggg cctcgacgaa cgccgcgcgc gtcgagcgtg gatcatcggc
     2401 cgggagcgta ccgaagtaca ggtcggggcg gaagaactca gcattggcac cgaaatgcgc
     2461 ggccgagagc accttgcgtc caggcggccc caggccgagc tcgaccacgg tggccgacgc
     2521 agccatcccg acagagagtg ccgcagccgc cagcagcgga atgccgtacg gggcccccat
     2581 gccgatcacc tcgccgggca gcttcgtcgc cggacggacc gaacctccga ggctcgcagc
     2641 ggcgcaggag gagtcggccg ctgttcgaag ataggcacag cgctgttggc gctggtcctc
     2701 aactgccgta ggagcgatga ccatggcatt caccgtgcgg aagatcaaga tacggcgcgc
     2761 gaaggtgaag aaccaggcag gggctctggc tcagttgttg gcaccggtgg cagaggccgg
     2821 cctcgacctc gagacggtgg tcgcgtacgc acagaagaag accggccctg cggacgtgta
     2881 ctgctcgctt gtggataccg gcaagaaggc cggcgcggct gcggacgcct gcgggttcgc
     2941 cgtcgacgat gcgtcgccgg ctctgctggt cgagggcgac aacggcccgg gcgtgctgag
     3001 gaaggtgacc caggcggtgg ccgatgcggg ggtcagcatg cgcgtctgca tcgcgctgcc
     3061 tgtcgggtgc aagttcgccg ctctgctggg ctttgacgac ccggccgacg ccgacaaagc
     3121 cgccaagaag atcagggcag ccggcagagc ctgacccgca cccgtccggg gcgcgggtgc
     3181 ctgccgaggc agatgggtgg tggccggggg cagaccgcga ctatgctgag cgcccggagc
     3241 cgcagtcggc ccccggcctt cgcctcccag cgccatcggt cgcgcggagg cctccgctcc
     3301 acgcggggcg aacccgcagt gcgcgcgatg caatgcgccg gtgcacagaa gtgagagggg
     3361 caccttcgtg tccacggacc ttgccaactt cagggctacg gtcgagcatc gccggcccga
     3421 acgcatcctc taccatgcag acttcgtcga agacctgcgg cgccgagttg tggactacat
     3481 tggcaccgag gacatcgccg gccacttcgg tttcttctct cccgtgggcc tcgggcccca
     3541 gcgccccgag ggccttccgc cactcgacta cacccgctac tgggaggggg aggaccttcc
     3601 cgagggcacg cagatcgacg gcaacggcgt cgccatggtg cccagcggca tgtaccactt
     3661 ctggggctac atctccccgc tgcgcaacgc ccagtcgttg gaggagatcg agtgctaccc
     3721 catggacgac atgcgcgact gggactggtc gcacctggac gccatcgtgg cagacgccca
     3781 tgcccagggc cgcgtcgtca gcgccttccc cgggcacatg tacgagaccg cctggcagat
     3841 ccgcggcctc gagcagttcc tggtcgacgt catcgagcgc ccggcatggg ccgagtgcct
     3901 gctggagcgc atcgccgagc agaaccagat acgcgcccgg gcctacgcca gagcgggggc
     3961 cgacctgatc cagtgtggcg atgacgtggc aagccaagtg gcgctgatat tctcacccac
     4021 cgtctggcgg aagatgatcc actcccgttg ggctcgcatc tgggccgaga tcaagagcat
     4081 caaccctcag tgcatcatct ggtaccacag cgatggcaac atcatcgaca tcgtgccaga
     4141 tatgggtgag aat
//
LOCUS       DAOSPL010000196         4137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21654_length_4137_cov_4.054140, whole genome shotgun sequence.
ACCESSION   DAOSPL010000196 DAOSPL010000000
VERSION     DAOSPL010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21654_length_4137_cov_4.054140"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1429
                     /locus_tag="PLQ54_04950"
     CDS             <1..>1429
                     /locus_tag="PLQ54_04950"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOS92637.1"
                     /translation="LVAGTYTLTPSKAEYTFAPGALEVTVGPDVAGQDFAATVVTYTI
                     RGTVTSAGKALSGVVLTADDGAGHVGTATTGADGKYIVSDLVAGTYTLTPTKPEYTFT
                     PVHSTVNLGPSISGEDFNGTLVTYSMSGRVTATGAGLAGVTVTVDNGAGGVKTATTTS
                     NGTWSLSGLLAGTYTVTPAKAQYTFTPETRSVLLGPSATAVDFEADLVTYSISGTIRL
                     NGQGLPGVEIVAGPGRTAITGPDGTYTVTGLLAGTHRVTPSMAEHAFDPPWLNVTVGP
                     DATGQDFSARIVTYSISGTISSGGVGLGGVIVEAGGQTAVSQASGAYTITGLPAGTYN
                     VTPASDDFVFDPPSAAVAVGPSVTDVNFTGDPRYRISGTVTAHGVPVAGVTVDAGGGH
                     TAITGADGTYTIAGLVAGEYTLTASKPEYTFVPATLKVTVGPSATGQDFTATQATYSI
                     GGTVTAHGVPVAGVTVDAGGGHTAIT"
     assembly_gap    1430..1529
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1530..2905
                     /locus_tag="PLQ54_04955"
     CDS             <1530..2905
                     /locus_tag="PLQ54_04955"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOS92638.1"
                     /translation="PEYTFVPATLKVTVGPSATKQDFAASRVYSRQFAAGWALFSVPV
                     STGGVTAGELFGDTRQYAAWWDAGTNSYAPLAGPPVLGRGYWFHVGSGGRTVQAAGTP
                     ASGREVEVALDAGWQMAGNPFEEAIDWSTTTVRVPGGAEVPLAQSSAVTPYAWAFDSE
                     TQSFMLVDPRVPGAETTIAPWSGFFVYAHSPVTLVLHRPGASTSSRSTVPLLRAESGG
                     FTINLSARCGTSADGFNVLGVRPQGASGDLRVAEPPPLSSDLSLTLIPPQASGGYAVD
                     MREKVSAADEWAYDAVVRSSAANRPVVLTWSDLRGLPREYEAVLTDVDAHESVLLRST
                     RAYTFRTGQNGTERRFRVAIRRNTAGLRIIAARADVLRSGGVAVAYSLTASATVSARV
                     LNAAGRVVAEPLRGETQVSGAQTLSWTGQGLSGSSLPPGQYVIELEAVGEAGARARAA
                     VRVTVGR"
     gene            2916..3578
                     /locus_tag="PLQ54_04960"
     CDS             2916..3578
                     /locus_tag="PLQ54_04960"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOS92639.1"
                     /translation="MWTRVRPVWIIIALSVAPVGCGGGPGEPGGTTYSISGSVTIDGA
                     GLAGVTVTAGGLSATTNAEGRYTIAGFSAGTYIVAASRSGYEISPASLTVTVGPSATG
                     RDFTAVAVGPSIETPTAALSVIDGIPMVTGAATVTYGTGEELEVEFVVTGPGGTAISL
                     PTVAGDGGHYQATYPIESLTPGSYSLAIRARVKGDPLRASELMLADAFLVPPRPPEEP
                     LT"
     gene            3665..>4137
                     /locus_tag="PLQ54_04965"
     CDS             3665..>4137
                     /locus_tag="PLQ54_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HOS92640.1"
                     /translation="MPEAIHLVALDLGAESGRAILATIDSGRLTLKPVHRFPNGPVRV
                     LNHLHWDVLRLYDEVKRGLALCSAEDDGPVMGLGVDTWGVDFGLLDRDGNLLGNPYHY
                     RDHRTEGMVEAACQRLSRREIFEQTGIQFMQLNTLFQLFSMGVTRSPLLDVADKLL"
BASE COUNT          646 a         1357 c         1404 g          630 t
ORIGIN      
        1 ctcgtggcgg gcacctatac gttgactccg agcaaggcag agtacacgtt tgctcccggg
       61 gcactcgagg tgaccgtcgg tccggatgtc gccgggcagg acttcgcggc gacggtagtc
      121 acgtacacca tccgcggcac cgtcacgagc gccggcaaag ccctgagtgg ggtcgtgctc
      181 acggccgatg acggggcagg tcatgttggg acggcgacca ccggggcaga tggcaagtac
      241 atcgtatcgg acctcgtggc gggcacctac acactaacgc cgacgaagcc cgagtacacg
      301 ttcacaccag tgcatagtac cgtgaacctg ggccccagca tctcgggaga ggacttcaac
      361 ggtacgctgg tgacgtacag catgtcgggg cgggtcacag ccaccggggc gggactggcc
      421 ggcgtcaccg tgacggtcga caatggggcc ggcggtgtca agacggccac tacaacatcg
      481 aacggcacat ggtcgctgtc gggtctcctg gcgggcacct acaccgtcac cccggcgaag
      541 gcgcagtaca ccttcacgcc ggaaacgcgc agtgtcctcc tcggcccgag cgccacggcg
      601 gtggacttcg aggccgactt ggtcacgtac agcatatcgg gcaccattcg actcaacggg
      661 caagggctgc ctggcgtcga gatcgtggcc ggcccgggcc gcacggcgat cacagggccg
      721 gacggcacgt acaccgtcac cggtttgctg gccggaaccc atcgggtgac acccagcatg
      781 gccgagcacg cgttcgatcc gccctggctg aacgtcaccg tggggccgga tgccaccggc
      841 caggacttca gtgcccgcat tgtcacctat tcgatatccg gcacgatctc gtccggcgga
      901 gtcggcctgg gtggggtgat cgtcgaggcc ggcggccaaa ccgcggtgtc tcaggcgagc
      961 ggtgcctaca cgatcacagg cctgcctgca ggaacgtaca acgtgacgcc ggcatcggac
     1021 gatttcgtct tcgatccccc gtcggccgct gtagccgtcg gcccgagcgt caccgacgtg
     1081 aacttcacag gcgacccacg ctaccgcatt agcgggacgg tgacggccca tggcgtgccg
     1141 gtggccgggg tcaccgtcga cgcgggcggc ggccatacgg ccatcacggg cgccgacggg
     1201 acctacacca tcgccgggct ggtggccggg gagtacacgc tgaccgccag caagcccgag
     1261 tacaccttcg tgccggccac actgaaagtc accgtggggc ccagcgccac gggacaggac
     1321 ttcacggcca cgcaggcgac ctacagtatt ggcgggacgg tgacggccca tggtgtgccg
     1381 gtggccgggg tcaccgtcga cgcgggcggc ggccatacgg ccatcacggn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gcccgagtac accttcgtgc cggccacact
     1561 gaaagtcacc gtggggccca gcgcaacaaa gcaggacttc gcggcatcgc gcgtctactc
     1621 gaggcagttc gcagcgggtt gggcgctgtt ctctgttccg gtgagcaccg gtggcgtaac
     1681 agcgggtgag ctgttcggcg atacgcggca gtacgcggcg tggtgggatg ctggcacgaa
     1741 ctcctacgcc cccctggccg ggccgccggt tctggggcgc gggtactggt tccatgtggg
     1801 ttcgggggga cgcacggtac aggcggcggg aacgccggca tcaggccgtg aggtcgaggt
     1861 ggcgctggac gccggctggc agatggccgg gaacccgttt gaggaggcca ttgactggtc
     1921 gaccaccacg gttcgcgtgc caggcggggc cgaagtaccc ctggcccagt cctccgcagt
     1981 cacgccatac gcatgggcgt tcgactcgga gacccagagc ttcatgctcg tggatcccag
     2041 agtgcccggt gcggagacga cgattgcgcc gtggtcgggc ttcttcgtct atgcccacag
     2101 cccggtgaca ctggtcctgc acaggccggg cgcctcgacg tcgagccgat cgacggtgcc
     2161 gctgctccga gccgagagtg gcgggttcac catcaacttg tccgcgcgat gcgggacatc
     2221 ggcggacggc ttcaacgtgc tgggcgtgcg gccgcagggg gcatccggcg acttgcgcgt
     2281 cgccgagccg cccccgctat caagtgacct aagtctcacc ctgatcccac cgcaggcctc
     2341 cggcggctat gccgtggaca tgcgggagaa ggtttcggcc gcagacgagt gggcctatga
     2401 cgccgtggtg cggtcttcag ccgcgaatcg gccggtcgtg ttgacctgga gcgacctgcg
     2461 cggcctcccg cgcgagtatg aagctgtgct gaccgacgtc gacgcccacg agagcgtcct
     2521 gctccgctcc acccgtgcgt acacgttccg gacggggcag aacgggacgg agcgccggtt
     2581 tcgcgtcgcg atccggcgga atacggccgg actccggatc atcgctgccc gggccgacgt
     2641 gctccgcagc ggcggggtgg cggtcgccta ctcgctgacc gcctcggcga ccgtctccgc
     2701 tcgggtgctg aacgcggccg ggcgtgtggt ggccgagcct ctgcggggtg agacgcaggt
     2761 gtcgggcgct cagacgctga gctggaccgg ccaggggctg tcgggttcgt cgctgccgcc
     2821 cggccagtat gtgatcgagc tcgaggccgt tggtgaggcg ggggcgcggg cccgggcggc
     2881 ggtacgcgtc acggtcggga ggtaggggca cctcgatgtg gactcgcgtc cgacccgtgt
     2941 ggatcatcat tgccttgtcc gtggcacccg tagggtgcgg cggcgggccg ggggagcccg
     3001 gcggcaccac ctacagcatc agcggctcgg tgaccatcga cggcgcgggg ctggccggcg
     3061 tcaccgtcac cgcgggcggc ctctctgcca ccacgaacgc cgagggccgg tataccatcg
     3121 cgggcttctc ggccggcacc tacatcgtgg ccgcctcgag gtcggggtac gagatcagcc
     3181 cggcctcgct cacggtgacc gtggggccca gcgccaccgg gcgggacttc acagccgttg
     3241 ccgtgggccc ctcgatcgag acgccgacgg cggcgctatc ggtcatcgac ggtatcccca
     3301 tggtcacagg cgccgccacg gtcacctacg gcaccggcga ggagctcgag gtcgagtttg
     3361 tggtcaccgg ccccggaggc accgccatca gcctccccac cgtggcaggc gatgggggcc
     3421 actaccaagc gacgtaccca atcgagagcc tgacacccgg gtcctactcg ctcgccattc
     3481 gcgcccgggt aaagggggat ccacttcgcg cctccgaact gatgctcgcg gatgccttcc
     3541 tggttccgcc ccggcccccc gaggagcccc tgacctaagc gccacaaccc aggcgtcgca
     3601 ggattcgcgg cggatctgac tgaacagaca ccccccgagg cacttccgcg gagggggatc
     3661 caccatgccc gaggccatcc atctggttgc gctggatctg ggagccgaga gcggccgcgc
     3721 catcctcgcg accatcgact ccggccgatt gacgctgaaa ccggtgcacc ggttcccgaa
     3781 tggtccggtg cgcgtgctga accatctgca ctgggacgtg ctgcggctct atgacgaagt
     3841 caagcgggga ctagccctct gctcggccga ggacgatggc ccggtcatgg gtctgggtgt
     3901 ggacacctgg ggcgtcgact tcggcctgct ggatcgagac gggaatctcc tggggaaccc
     3961 ctatcactac cgcgaccatc gcaccgaggg catggtggaa gccgcgtgcc aacgtctttc
     4021 acggcgcgag atattcgagc agaccggcat ccagttcatg cagctgaaca cgctcttcca
     4081 gctcttctcc atgggtgtta cccgatcccc actgctggac gtcgccgaca agctact
//
LOCUS       DAOSPL010000197         4136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21664_length_4136_cov_2.579270, whole genome shotgun sequence.
ACCESSION   DAOSPL010000197 DAOSPL010000000
VERSION     DAOSPL010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21664_length_4136_cov_2.579270"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /locus_tag="PLQ54_04970"
     CDS             <1..151
                     /locus_tag="PLQ54_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011705411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOS92641.1"
                     /translation="DEMLRMVDALQFTEEHGEVCPAGWMKGKKGMTASPDGVDKYLAE
                     NAKEL"
     gene            complement(224..697)
                     /locus_tag="PLQ54_04975"
     CDS             complement(224..697)
                     /locus_tag="PLQ54_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HOS92642.1"
                     /translation="MANVLFAGVGGQGVVTASDLLAHAALRAGWNVKKAETHGMAQRG
                     GSVVTHVRVSADQPVHSRLIPHGCADYLIALELLEGVRTLWMLAPGGDSFGIDVPHAH
                     WHTVVALQPGTVIFEAKSGPYQPLLPEEKAPWAPVEGAAGVDVYLRQIESLFPTG"
     gene            complement(697..2493)
                     /locus_tag="PLQ54_04980"
     CDS             complement(697..2493)
                     /locus_tag="PLQ54_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOS92643.1"
                     /translation="MLLSGNEAVALGAWQAGCSLAAAYPGTPSSEILPALIRWAERHG
                     GDIYCEWSVNEKVALEVASGASIAGARTLAAMKHVGLNVAADPFFTLAYTGVEGGLLV
                     ISADDPGLHSSQNEQDNRIYARHAGIPMLEPADSQEACDFARLAFELSERFDVPVLLR
                     LTTRVSHSRTPVVAGLERAEREVTGATPNPQKYVMVPAHAKTRHEVLQRSLARVARWG
                     DEAPVNRVDDGVGDVAFIASGLAYCLLREVLPEAPVLKLGMTYPLPGSLIGGFVERFA
                     RVVVVEELEPFLFEQIRALGHAVDRLPAELQLGELTPDRLVHGLRQIGIEPTGLSPAP
                     DEALGPPAGLPARPPVLCPGCAHRAVFAVLRKLKVFVTGDIGCYTLATLEPLAALHTC
                     LDMGAGISQAHGISRATGGKQKAVAVIGDSTFTHMGMPALLNAIYNGGDFVTLILDNR
                     STAMTGGQDHPGTGTTLRGESSRALDFARLAEAMGAEMVMQVPVSDPKAIEAGLRQAL
                     NHRGPAVVIASGLCPLRYRVRTTPYAIDREKCTLCRRCLALGCPALGLSVDSGLKWPV
                     IDAALCNGCGLCAQECLLGAISLADANGGDAV"
     gene            complement(2602..3255)
                     /locus_tag="PLQ54_04985"
     CDS             complement(2602..3255)
                     /locus_tag="PLQ54_04985"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS92644.1"
                     /translation="MHRSIVAVALGLVVGVWIAVAPRPASADADVAEPEDEGWIALFD
                     GETLDGWDRTGNAEWSVEDGILVGKQGPDGAPGDIWTREAYKDFELECTFKVIWPANS
                     GIWFRSDQSHGKLGYQVDILRMEEYGCTVGTIYSDGFLSQNKDQDLVRLDDWNTVKLT
                     ARGYHFRVVLNGHEVGDVTDEQSRYAEGRIGFQVHAGDQYKDMRIMVKELKLRPLED"
     gene            complement(3359..3814)
                     /locus_tag="PLQ54_04990"
     CDS             complement(3359..3814)
                     /locus_tag="PLQ54_04990"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOS92645.1"
                     /translation="MQATIQERKGFTVLGILVTIDPMAADYNDIWRNQYEPRADEIRA
                     HATDEACFGIYFDCGKPGMVDFVAGAPVAGVGAPPEGLVLREIGPCTEAVFECSMAAI
                     GQTWGAIFGQWMPASGYRIDESSPCLERFAPGCHEGTVPVTIHVPVRKA"
     gene            complement(3900..>4136)
                     /locus_tag="PLQ54_04995"
     CDS             complement(3900..>4136)
                     /locus_tag="PLQ54_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92646.1"
                     /translation="KQNSLLKQHPFVAYKALQAAAGFTAEELAEALGHLLNLDAALKS
                     TTGLEDIALLELVIIELCGQHGRGAAAIVARALD"
BASE COUNT          712 a         1387 c         1351 g          686 t
ORIGIN      
        1 cgacgaaatg ctgcgcatgg ttgatgccct gcaattcacc gaggagcacg gcgaagtctg
       61 cccggcaggc tggatgaagg gcaaaaaagg catgaccgcc tctccggacg gcgtcgacaa
      121 gtacctggcg gagaacgcca aggaactgta attccggcag acgccaacaa aaagccgctc
      181 atccgagcgg cttttttcat ggcactgcaa tctgctgaaa aaatcagcct gttgggaaaa
      241 ggctttcaat ctggcgcaga tacacgtcga cacctgcggc gccttcgacc ggcgcccatg
      301 gcgccttttc ctcgggaagc agcggctgat acgggccgga cttcgcttcg aaaatcaccg
      361 ttcccggctg cagagccacc actgtatgcc agtgtgcatg cggtacgtcg atgccaaagc
      421 tatcgccgcc cggcgccagc atccacaatg tacgcacgcc ctcgagcaac tcgagggcga
      481 tcaggtagtc cgcacagccg tgggggatca gtcgcgagtg aaccggctga tcggccgaca
      541 cgcgcacatg ggtgaccacg ctcccgccac gctgggccat gccgtgcgtc tctgccttct
      601 tcacgttcca ccctgcccgt aatgccgcat gggccagtag gtcgctggct gtcaccacgc
      661 cctggccgcc cacgccggca aacagcacgt tggccatcac accgcatccc ccccgttcgc
      721 gtcggccagt gagatggctc ccagcaggca ctcctgagcg cagagcccgc agccgttgca
      781 gagcgccgcg tcgatcaccg gccatttcaa cccggagtcg acggacagcc ccaacgccgg
      841 acaccccaag gccaggcagc gccggcagag ggtgcacttc tcccgatcga tagcataggg
      901 cgtggtccgc acccggtagc gcagcgggca cagtcccgag gcaatgacca ccgcaggccc
      961 ccggtgattc agcgcctgac ggaggcccgc ttcgatggcc ttcgggtcac tgaccggaac
     1021 ctgcatgacc atctcggccc ccatggcttc cgccaacctt gcgaagtcga gtgcccggct
     1081 gctctcacct cggagggtgg tcccggtccc gggatggtcc tgcccgcccg tcatcgccgt
     1141 gctgcggtta tccagtatga gagtcacgaa gtcgccgccg ttgtagatgg cgttgagcag
     1201 agcaggcatt cccatgtgtg tgaaagtcga gtcgccgatc acggccacag ccttctgttt
     1261 gccacccgtt gccctcgaga tgccgtgagc ctggctgatg cccgccccca tgtccaggca
     1321 cgtgtgcagg gctgccagcg gctcgagtgt ggccagtgtg tagcagccga tatcccccgt
     1381 cacgaaaacc ttgagcttcc gcagaacggc gaacacggcg cggtgcgcgc agcccggaca
     1441 gagcaccggc ggccgcgcgg gcaggccggc cggagggccc agtgcttcgt ctggtgccgg
     1501 actcagcccc gtgggctcga taccgatctg gcggaggcca tggaccagac ggtcgggagt
     1561 caactcgccc agctgcagtt cggcgggcag acggtcgacg gcgtggccca gggctcggat
     1621 ctgctcgaag aggaacggct caagctcctc gaccacgacg acgcgtgcga agcgctcgac
     1681 gaacccaccg atcaggcttc ccggcaacgg gtaggtcatc ccgagcttga ggacgggcgc
     1741 ctccggcagc acctcgcgga gcaggcagta ggcgagtccc gaggcgatga aggcgacgtc
     1801 ccccaccccg tcgtccacgc ggttcacggg cgcctcgtcg ccccagcggg ccacacgggc
     1861 caagctgcgc tggaggacct cgtgcctcgt cttggcgtgg gcgggcacca tcacgtactt
     1921 ctgggggttg ggagtggcac cagttacctc gcgctcggcc cgttccagac cggccacgac
     1981 gggggttctc gaatgggaca cgcgggtggt gaggcgcagc aggaccggca catcgaagcg
     2041 ctcgctcagc tcgaaggcca gccgcgcgaa gtcacatgcc tcttggctgt cggcgggctc
     2101 cagcatcggg atgcccgcat gtcgcgcgta gatgcgattg tcctgctcgt tctgcgagga
     2161 gtgcaggccg gggtcatcgg ctgatatgac cagcagacca ccctcgacgc ccgtgtaggc
     2221 cagagtgaag aacggatcgg ccgcgacgtt caggcccaca tgcttcatgg cggccagcgt
     2281 gcgggccccg gcgatcgagg cacctgaggc gacttcgaga gcgactttct cgttcaccga
     2341 ccactcacag tagatgtcgc caccgtgccg ttcggcccaa cgtatcaggg ccggcaggat
     2401 ctccgacgag ggggtgccgg ggtaggcggc ggccaggctg cagccggcct gccaggcacc
     2461 cagggcgacg gcctcgttgc ccgagagcag catgggctgg ggttgttcag acaatgacgg
     2521 ctcctacatc gaccacccct cagtccgcca cgcgggccgt ggcgagggct ggtgcggtat
     2581 tggggacggc ggcgagggcg gctagtcctc cagcggacgc agcttgagct ccttcaccat
     2641 gatgcgcata tccttgtact gatcccccgc atggacctgg aagccgatcc ggccctcggc
     2701 gtagcgggac tgctcatcgg tgacgtcacc cacctcgtgg ccgttgagca ccacccggaa
     2761 gtggtaccct cgggcggtga gcttcacggt gttccagtcg tccagtctga ccaggtcctg
     2821 gtctttgttc tgtgagagga agccatccga gtagatggtg cccaccgtgc aaccgtactc
     2881 ctccatgcgg aggatgtcga cctggtagcc gagcttaccg tgggactggt cggaccggaa
     2941 ccagatgccg ctattggcgg gccagatcac tttgaaggtg cactcgagct caaagtcctt
     3001 gtaggcctct cgggtccaga tgtccccggg tgcgccgtca gggccctgct tgccgaccag
     3061 aatcccgtcc tcaaccgacc actcggcatt gccggtccgg tcccacccgt cgagcgtctc
     3121 gccatcgaac agggcgatcc agccctcgtc ctcgggttcg gccacatcgg catcggccga
     3181 cgcggggcgt ggcgcaacgg cgatccagac gccgaccacc aagcccagag ccaccgccac
     3241 gatactccgg tgcattccag gtcatctccc atcaggcatg cgcggccgct gggccggcca
     3301 accgaccgga ttctacgcgg ggccccaggg agggtcaaac cgactgggcg tggatgcctc
     3361 aggccttccg cacgggcacg tggatcgtca cgggcactgt accctcgtga cagccaggcg
     3421 cgaaccgttc gaggcaggga ctggactcgt cgatgcgata ccccgaagct ggcatccact
     3481 gaccgaagat ggcgccccag gtctggccga tggcggccat actgcactcg aagaccgcct
     3541 cggtgcaggg cccgatctcg cggagcacga gcccttcggg cggcgcaccg acacccgcta
     3601 cgggcgcgcc ggcgacgaag tcgaccatac cgggcttgcc gcagtcaaag tagatgccaa
     3661 agcaggcctc atcggtggcg tgggcgcgga tctcgtcggc gcgcggctca tactggttcc
     3721 gccagatgtc gttgtagtcg gcggccatgg ggtcgatcgt gaccagaatg ccgaggacgg
     3781 tgaagccctt gcgctcctgg atcgtggcct gcatggatgc cccctctgtt tcggctcggc
     3841 tgcaattgac cgggcgcacg cattggctct tcgccgtgcc ggcgagtgag gccttccgtt
     3901 cagtcgagtg ccctggccac gatggcagcg gcgcctcggc cgtgctggcc gcagagctcg
     3961 atgatgacca gctcgagcag agcgatgtcc tcgaggccag tggtcgactt gagggctgca
     4021 tcgaggttca ggaggtgtcc cagggcctcg gcgagttcct cggcggtgaa tcccgcggct
     4081 gcttgcaggg ccttgtaggc cacgaagggg tgctgcttga gtaggctgtt ctgctt
//
LOCUS       DAOSPL010000198         4124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21730_length_4124_cov_2.806095, whole genome shotgun sequence.
ACCESSION   DAOSPL010000198 DAOSPL010000000
VERSION     DAOSPL010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21730_length_4124_cov_2.806095"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1359)
                     /gene="wecC"
                     /locus_tag="PLQ54_05000"
     CDS             complement(91..1359)
                     /gene="wecC"
                     /locus_tag="PLQ54_05000"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HOS92647.1"
                     /translation="MKPGFERVCVMGLGYIGLPTASLIANRGFHVRGVDVNRETVETI
                     NRGGVHIVEPDLDILVRSAVGSGQLVADTEPAPADVFIIAVPTPICPSHGPDLTYVEQ
                     ATRAIASHVAPGNLVILESTSPVGTTEKVAAWLSEERPDLRTPVATDGASDTTPREAQ
                     VFIAHCPERVLPGQILRELVDNDRVIGGIDRASAERAREFYECFVSGEIVLTTARTAE
                     LCKLTENASRDVGIAFANELSLICEKLGVNVWELIGLANRHPRVNILRPGPGVGGHCI
                     AVDPWFIVDSAPEEARLIRTAREVNDGKPHHVVRSIVAAAARFRDPVIACLGLAFKAN
                     VDDLRESPAVEIVKELAERHAARVLVVEPHIRKLPAPLSALGVELVPLGAALEQADVV
                     ALLVDHRVFASVDKEVLLRKVVIDTRGLWQ"
     gene            1545..1922
                     /locus_tag="PLQ54_05005"
     CDS             1545..1922
                     /locus_tag="PLQ54_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HOS92648.1"
                     /translation="MRIRIAAGDVEALAELNDSPTALEIIERLPIYARGQRWGDEIYF
                     PIPVHMDEEPGAREMVDVGALAYWPPGNAFCIFFGRTPASDGSEPCAASPVNLIGRVL
                     TDPTEFKRVVSGEAVSIEVVPEG"
     gene            2049..3581
                     /gene="xylB"
                     /locus_tag="PLQ54_05010"
     CDS             2049..3581
                     /gene="xylB"
                     /locus_tag="PLQ54_05010"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOS92649.1"
                     /translation="MGYLLGIDIGTSGTKALLIDETGRVLASATEEYPLLTPRPGWAE
                     QHPQDWWRATADATRAVLAKAGVTGADVAGVGLSGQMHGSVFLDANNEVIRPALLWCD
                     QRTAAQCQWINSTAGEGLVVEETLNPMLTGFTAPKIIWLRQEEPEHYARVRKVLLPKD
                     YIRWMLSGEFASEVSDASGTALLNVRQRQWSTRLMNAIDIDPAWMAPVFESQEPSTRV
                     SARAAEATGLAAGTPIVGGGGDQAAGAVGNGIVRTGVVSSTTGTSGVVFAFMDEPLVD
                     PRLRAHTFCHAVPGKWHTMGVMLSAGGSLRWIRDALCGPERDVAAAMGKDPYEVMCEE
                     AAAAPPGCEGLVFLPYLTGERTPYPDPDAKGVFFGLHLRHTRAHLIRAVLEGVAFGLK
                     DSFEIFAEMGVPVDQVRASGGGARSKLWLQIQADATNHAHHTINVEEGPALGVALLAG
                     VGAGVYRSVAEACDAVIRLASETKPIPGNAELYSRCHALYQRLYGHLASDFKEASALA
                     AG"
     gene            3791..>4124
                     /locus_tag="PLQ54_05015"
     CDS             3791..>4124
                     /locus_tag="PLQ54_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92650.1"
                     /translation="MRPARTDDLIRQIAALDIIDCREHLPPEKAYLETPTDACVLFGH
                     YCRTDLITAGMAPGDYDRMLDRSLPLDYRWGLLAPHMEKVRHGSYARAAFIAAREVYG
                     FEDITSANY"
BASE COUNT          639 a         1468 c         1362 g          655 t
ORIGIN      
        1 gtgctccgcg cgcccccttg gttcaggtgt caccctgagg accggaggga cgaatgatct
       61 ccgctagcac cagcttccgg gcggctgccc ctactgccac aacccccggg tgtcgataac
      121 gaccttccgc agcaggacct ctttgtcgac ggaagcgaac acgcgatggt ccaccagcag
      181 cgccaccacg tctgcctgct ccagcgccgc gcccagtggc acaagctcga caccgagggc
      241 actgagtggc gcgggcagct tacgaatgtg cggctccacg accagcacgc gtgccgcgtg
      301 cctctcggcc agctccttca cgatctcgac ggccgggctc tcacggagat catccacatt
      361 cgccttgaac gccagcccga ggcacgcaat gaccgggtcc cggaagcggg cggccgccgc
      421 gacgatgctt cgcaccacat ggtgcggctt gccgtcgttg acctcgcggg ccgtgcggat
      481 cagccgcgcc tcctcgggcg ccgagtccac gatgaaccac gggtcgaccg cgatgcagtg
      541 tccacccaca cccggccccg gccgaaggat gttcacccgc gggtgacggt tcgcgagccc
      601 gatcagctcc cacacgttca caccgagctt ctcgcagatg agtgatagct cgttggcgaa
      661 ggcgatgccc acatcgcggg aggcgttctc agtcagcttg cacagctctg ccgtgcgcgc
      721 cgtggtgagc acgatctcgc cggagacgaa gcactcatag aactcccgcg cccgctcggc
      781 cgaggcccga tcgatgccgc cgatgacgcg gtcattgtcg accagctccc gcaggatctg
      841 gccggggagc acgcgctccg ggcagtgggc gatgaacacc tgcgcctccc tcggtgtcgt
      901 gtcggaggct ccgtcggtgg cgaccggcgt gcggaggtcc ggacgctcct cactgagcca
      961 cgccgccacc ttctccgtcg tgcccaccgg gctcgtggac tcgagaatca ccaggttccc
     1021 tggcgccaca tgggaagcga tagcccgtgt agcttgctca acataggtca gatcgggccc
     1081 gtggctggga cagatgggtg tcggcacggc gatgatgaac acgtcggccg gcgcgggctc
     1141 cgtgtcggcc acgagctggc cactgcccac ggcggagcga accagaatgt ccagatcggg
     1201 ctcgacgatg tggacgccgc cgcggttgat ggtctccacc gtctcgcggt tcacatcgac
     1261 cccacggacg tggaaccccc ggttggcgat gaggctggcc gtcggcaagc cgatgtagcc
     1321 caggcccatc acacagactc gctcaaagcc cggtttcata ctgtctctcc cgtggtgaga
     1381 tgtggcccgc cgtcatcggg tcagccggga cagacccttt cgtgcgcccg aacgccttcc
     1441 cttctcgtcg tggtcgccac cgatgtaggg aacgcctcag cctatggcga acgcgccgcc
     1501 ccgcgggagc cacgctcggc tccgcgcgga ggtgtgttcc cttggtgagg atccggatcg
     1561 ccgcgggcga tgtcgaggcc ctggcggagc tcaacgactc ccccaccgcc ctcgagatca
     1621 tcgagcggct acccatctac gcccggggcc agcgctgggg cgatgagatc tacttcccca
     1681 tccccgtgca tatggacgaa gagcccggcg cgcgcgagat ggtcgatgtg ggcgcactgg
     1741 cttactggcc gcccggcaac gctttctgca tcttcttcgg ccgcacgccg gctagcgatg
     1801 gttcggagcc ctgtgcagcc agccccgtga acctcatcgg gcgggtcctg actgacccga
     1861 ccgagttcaa gcgcgttgtc tccggcgaag ccgtctccat cgaggtcgtg ccggagggct
     1921 gattgggcgg gcgaggacgc ccgccggcac tggagctgtc ccattcgtgc ccgcgggcct
     1981 cccggcccgc atcggcacca tccgggcgct cgggcgcccc acaagtcatg accgcgaggt
     2041 gagtccgcgt gggatacctg ttgggcatcg atatcggtac cagcggcacc aaggccctgc
     2101 tcatcgatga gaccggccgg gtgctcgcca gcgccaccga ggagtacccc ctgctcacgc
     2161 cgcgccccgg ctgggccgaa cagcacccgc aggactggtg gcgggccacg gccgatgcca
     2221 cccgcgccgt tctggccaag gcgggcgtca ccggggccga cgtggccggt gtcggccttt
     2281 cgggacagat gcatggctcc gtcttcctcg atgcgaacaa cgaggtcatc cggccggccc
     2341 tgctctggtg cgatcagcgc acggccgccc agtgccagtg gatcaactcg accgccggtg
     2401 agggactggt ggtcgaggag acgctgaacc ccatgctcac cggcttcacc gcccccaaga
     2461 tcatctggct gcgacaggaa gagcctgagc actacgctcg cgttcgcaag gtgctgctgc
     2521 cgaaggacta catccggtgg atgctctccg gcgagttcgc ttcggaggtc tccgacgcgt
     2581 ccggcaccgc cctgctcaac gtgcgccagc ggcagtggtc gacacggctg atgaatgcca
     2641 tcgacatcga tccggcctgg atggccccgg tgttcgagtc gcaggaaccc agcacacgtg
     2701 tcagcgcaag ggcggccgaa gctaccgggc tggcggctgg gacacccatc gtcgggggcg
     2761 ggggcgacca ggcggccggt gccgtgggca acggtattgt ccgcaccggt gtggtctcgt
     2821 caaccaccgg cacctccggc gtcgtgttcg ccttcatgga tgaaccgctc gtggatccgc
     2881 gccttcgcgc ccacaccttc tgccacgccg tgccgggcaa gtggcacacc atgggggtca
     2941 tgctctcggc cggcggctcg ctccggtgga tccgcgacgc cctctgcggc cccgagcgcg
     3001 acgtcgccgc agccatgggg aaggatccct acgaggtcat gtgcgaggag gccgccgcgg
     3061 cgccgccggg ctgcgaggga ctggtgttcc tgccttacct caccggcgag cggacaccgt
     3121 accccgaccc cgatgccaag ggcgtgttct ttggcctgca tctccgccac acccgcgcgc
     3181 atctcatccg tgcggtgttg gagggcgtcg cattcgggct caaggactct ttcgagatct
     3241 tcgccgagat gggcgtccca gtcgatcaag tccgcgcctc cggcggcggc gcccgcagca
     3301 agctgtggct ccagatccag gccgatgcca ccaaccatgc gcatcacacg atcaacgtcg
     3361 aagagggccc ggccctcggt gtcgccctcc tggccggcgt gggcgctggg gtctaccgct
     3421 cggtcgccga ggcttgcgat gcggtgatcc gacttgccag cgagaccaag cccatccccg
     3481 gcaacgccga gctctacagc cgctgccacg cgctgtacca gcgcctctac ggccacctgg
     3541 cgagtgactt caaggaagct tcggccctgg cggcggggta ggttcgccga gggtggggca
     3601 gcgcatgccg gcgcggcagc ccggtgagag tccagacgca ccggtccctc tggcggggcg
     3661 tggggcgaag ccccgctggc aattgaggtt ggggggagcc cggcggcccc gcggctgctc
     3721 gccgtaggcc atcccccgca ggccggcgaa ctccccacgc atccgcaccc acgccagggg
     3781 gtgatccggt atgcgtccgg ctcgaaccga tgatctgatc cgccagatcg ccgcgctcga
     3841 catcatcgac tgccgcgagc acctgccgcc ggagaaggcc tacctcgaga cgcctaccga
     3901 tgcgtgcgtg ctcttcgggc actactgccg gacggatctc atcacggcgg ggatggcccc
     3961 gggggactat gaccgtatgc tcgaccggag cctgccgctg gactaccgct ggggcttgct
     4021 ggcaccccac atggagaagg tccggcacgg cagctacgcc cgggctgcct tcattgcagc
     4081 ccgcgaggtc tacggcttcg aggacatcac cagtgcgaac tacg
//
LOCUS       DAOSPL010000199         4118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21770_length_4118_cov_3.102387, whole genome shotgun sequence.
ACCESSION   DAOSPL010000199 DAOSPL010000000
VERSION     DAOSPL010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21770_length_4118_cov_3.102387"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="PLQ54_05020"
     CDS             complement(<1..834)
                     /locus_tag="PLQ54_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92651.1"
                     /translation="MWLTLAIAGIVASSSVPWIGYTEYRTNLPGGRYANVATMRACVV
                     RADGRGRKALCPELAAPLGAWTQFAGWSPDGRTAIIGSGWESAGNAAWEEAHGTFRFD
                     EGAWRYDTLLVDMATGVATNVTAVDRVSHYNSGVFYWPDDPTRLGFTALIGGESHPFS
                     MDLDGRHKTDLSGGVAGFTYGFSASPGGHRIAYHRDYQVFLADADGGNAARVDTGNPF
                     NFAPQWSPDGRWLAFVSGEHYDCHPYIVRADGTGLRKLADRGGFEGVVAFLDVPDFHG
                     GS"
     gene            998..2044
                     /locus_tag="PLQ54_05025"
     CDS             998..2044
                     /locus_tag="PLQ54_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS92652.1"
                     /translation="MSRQLALDTLWLRPTPRLAHTEYSVEYHHDYITRITGLPEGHPN
                     RIARLRELWGFDFLWSVDDGLHGNWFQFGRATDMGHAEYAADGSDLHQPTESPFHTAE
                     DVWAFDPVAEYGLPDFAEQVAAYERSMQTARQSAPEQLMTGGYYKTCVSGAIQAFGWD
                     MFLLAAADRTRIAKVLERILEFTRFHMNAWAETSAEAIIQHDDFVWTEGAFMHPSLYR
                     EVIIPGFAELWKPLKAAGKKIIFCSDGNFMEFAEDIIAAGADGLSFEPCNEFGWMAER
                     FGDSTVLIGSDVDCRDLAFGTWDAVRASMDRTLELAKRCQGLIWAVGNHMPANIPGEM
                     MDRYIEYLQANWWR"
     gene            complement(2069..2905)
                     /locus_tag="PLQ54_05030"
     CDS             complement(2069..2905)
                     /locus_tag="PLQ54_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS92653.1"
                     /translation="MKIAIIPTTGGADPWRGIEVAVELGVEGVHIGGYGGPWDLEHRT
                     PSERRGILERIRSYGLEVSALIGWGGNVDLCEEENWEENIAWGQRLNETAVDMSGGLW
                     MAHAGICPEDESSPRYMRFREGLKRIADHGERVGAIMALETGPEPPRVVRKLMEDIGS
                     PALRVNFDPANLILWPPHIAKEHGVPFDYQWAMDNFDPVEGLRTLIPFVAHSHAKDSV
                     LRPDGTCEEVPLGTGMTNWPELHRVFVENGYRGFYAIERECGEDAMGDVRKAVEYLKG
                     LD"
     gene            3093..>4118
                     /locus_tag="PLQ54_05035"
     CDS             3093..>4118
                     /locus_tag="PLQ54_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOS92654.1"
                     /translation="MRIWNEEAECQPRAERAEEQTRNLERLVNRVYDDLPYYRTKMDE
                     AGLRPAHIRSLRDLSKLPFTTKEDMRANYPYGLFAVPLCDVVRIHSSSGTTGRPTVVG
                     YTRRDLEIWAEVCARFITAAGVGSDDVAQVSFGYGLFTGAFGLHYGLERVGATVVPAS
                     SGNTARQLLVMHDFGVTTLVCTPSYACHLAEAAEQQGLAGKLKLRTGLFGAESWTDQM
                     RQDLEAKLGIAATDNYGLSEVIGPGVAGECLEARNGMHIAEDHFLCEIINPDTLEPVE
                     MGETGELVITTLTKEAVPIIRYRTRDITRLDPEPCACGRTTIKMHRVVGRSDDMLVVR
                     GTNVFPSQ"
BASE COUNT          728 a         1386 c         1329 g          675 t
ORIGIN      
        1 gctcccgccg tggaagtcgg ggacgtcgag gaatgccacc acgccctcga aaccgccgcg
       61 gtcggccagc ttccgcagtc cggtgccatc ggcacggacg atgtacggat ggcagtcgta
      121 gtgctcgcct gagacgaaag ccagccaccg cccatccggc gaccactggg gcgcgaagtt
      181 gaacggattg cccgtgtcga cccgggcggc gttgcccccg tcggcatcgg ccaggaagac
      241 ctggtagtcg cggtggtagg cgatgcggtg accacccggc gacgccgaga agccatacgt
      301 gaaccccgcc actccaccgg agaggtcggt cttgtgccgt ccatcgaggt ccatgctgaa
      361 gggatgcgac tcgccgccaa tgagcgccgt gaacccgagc cgagtcgggt cgtcgggcca
      421 gtagaacacg ccggagttgt agtgactcac gcggtcgacg gcagtcacat tcgtcgcgac
      481 acccgtggcc atgtcgacga gcagcgtgtc gtaccgccag gcgccctcgt cgaagcggaa
      541 ggtcccgtgg gcctcctccc aggcggcatt ccccgcactc tcccagccac ttccgatgat
      601 ggccgtgcgg ccgtcgggcg accaaccggc gaactgcgtc caggcgccca gaggcgctgc
      661 caactccggg cacagggcct tgcggccccg gccatcggcg cggacgacgc aggcccgcat
      721 ggtcgccacg ttcgcgtaac gccctccggg aaggttggtc cgatactccg tgtagccgat
      781 ccagggcaca ctcgaactcg ccacaatgcc ggcgatcgcc aaagtcagcc acatgggctc
      841 catcgcgccc gtcgcctcac tcgtgctggc tggctcggac gacgtgcgag caccccttgc
      901 atctcaggcc gtgaacagga gtgccgccgt cggccccgaa ccactccgtc agtaccacac
      961 accgcgccct ctggggcgcc gaacgaggtt gtgactcatg agccggcaac tggcactgga
     1021 caccctctgg ctccggccca ccccgagact ggcccatacg gagtacagcg ttgagtacca
     1081 ccatgactat atcacgcgga tcaccggact gccggaaggc catcccaacc gcatcgccag
     1141 actccgcgag ctctggggct tcgacttcct ctggagcgtc gacgacggcc tccacggcaa
     1201 ctggttccag ttcggtcgcg ccaccgacat gggacatgcc gaatacgccg ccgatggcag
     1261 cgatctacac cagcccacgg agtcgccgtt tcacacggcc gaggatgtct gggcgttcga
     1321 tccagtggcg gaatacggcc tgccggactt cgccgaacag gtggcggcct atgagcgctc
     1381 aatgcagacc gcgcggcagt cagcgcccga acagctcatg actggcgggt actacaagac
     1441 ctgcgtctcc ggagccatcc aggccttcgg gtgggacatg ttcctccttg cggccgcaga
     1501 ccggacacgg atcgcgaagg tgctggagcg catcctggag ttcacacgct tccacatgaa
     1561 cgcgtgggcg gaaaccagcg ccgaggctat catccaacac gatgacttcg tctggaccga
     1621 aggcgccttc atgcacccaa gcctgtaccg cgaggtgatc atccccggct tcgcggagct
     1681 gtggaagccg ctgaaggctg cgggcaagaa gatcatcttc tgttccgacg gcaacttcat
     1741 ggagttcgcc gaggacatca tcgctgccgg cgccgacggt ctgagcttcg agccctgcaa
     1801 cgagttcggg tggatggccg agcgtttcgg cgactcgaca gtgctcatcg ggagtgatgt
     1861 cgactgccgt gacctggctt tcggcacatg ggacgcggtt cgggcgtcca tggaccgcac
     1921 gctggagctc gccaagcgct gccagggcct catctgggcc gtgggtaacc acatgcccgc
     1981 caacatcccc ggtgagatga tggaccggta tatcgagtac ctgcaggcga attggtggcg
     2041 atagcgtccc cgcgccattc cgtcgccgct agtcgagacc tttgaggtac tccaccgcct
     2101 tgcggacgtc gcccatggcg tcctcgccgc actcgcgctc gatggcgtag aagccccggt
     2161 acccgttctc gacgaagacc cggtgcagct cgggccagtt ggtcatgcca gtgcccagag
     2221 gcacttcctc acaggtgccg tccgggcgga ggaccgagtc cttagcgtgg gagtgggcca
     2281 cgaaaggaat cagcgtccgc agaccctcca cggggtcgaa gttgtccatg gcccactggt
     2341 agtcgaacgg caccccgtgc tccttggcaa tgtgaggcgg ccagaggatc aggttggcgg
     2401 ggtcgaagtt cactcgaagg gctgggctgc cgatgtcctc catgagcttc cgaaccaccc
     2461 ggggcggctc gggccccgtc tcgagggcca tgatggcccc cacgcgctcg ccatgatccg
     2521 cgatgcgctt aagcccctcg cggaaccgca tgtagcgggg gctggactcg tcctcggggc
     2581 agatgccagc atgcgccatc cacaacccgc cggacatgtc gacggccgtc tcgttgagac
     2641 gctggcccca ggcgatgttc tcctcccagt tctcctcctc gcaaagatcc acattgccgc
     2701 cccacccaat gagtgcggag acctccaaac cgtagctccg gatgcgctcc aggatgcctc
     2761 tgcgctccga cggcgtgcgg tgctccaggt cccacggtcc gccatagccg ccgatatgca
     2821 cgccctcgac gcccagctcc actgcgacct cgatcccacg ccagggatcg gcgccgcctg
     2881 tggtggggat aatcgcgatc ttcatgcctc gcgtcctccg tctcagccca gcctcccact
     2941 gccggcagtg gcacgccggt gttcgcgagg cgtagactaa gcccctgcgt ctgcatggga
     3001 gacgccgggg tccggagagg accgcccccg tgggcttgag aagggttgac ccgaaagggt
     3061 ctgcagccac tgattgcgta aggaagtccg ccgtgaggat ctggaacgag gaagccgagt
     3121 gtcagccacg cgccgagcgc gccgaggagc agacgcggaa tctggagcgg ctggtcaatc
     3181 gcgtgtatga cgacctgccc tactatcgga cgaaaatgga cgaggccggc ttgcggcccg
     3241 cgcacatccg gtcactgcgt gatctcagca agctcccgtt cacaaccaag gaggacatgc
     3301 gggccaacta cccctacggg ctgttcgccg ttccgctgtg cgatgtggtg cggatccact
     3361 cgtcgtcggg caccacggga cggccgacag tggtcggcta cacccggcgg gacctcgaga
     3421 tctgggccga ggtgtgtgcg cgtttcatca ccgcagcagg agtcggctcg gatgatgtcg
     3481 cccaggtgtc cttcggctac ggcctgttca ccggcgcttt cggtttgcac tacgggctcg
     3541 agagggttgg ggccacggtg gtaccagcct cgtcgggcaa caccgcgcgg cagctgctgg
     3601 tgatgcacga ttttggtgtc accaccctcg tgtgcacacc gtcctacgca tgtcatctgg
     3661 ctgaagcggc ggaacagcag gggctggccg gcaagctcaa gctgcgaacg ggcttgttcg
     3721 gcgccgagtc gtggaccgac cagatgcgcc aggacttgga ggcgaagctc ggtatcgcgg
     3781 ccacggacaa ctatgggctg agcgaggtca tcgggcccgg ggtggcgggc gaatgcctcg
     3841 aggcgcgcaa cggcatgcac atcgccgagg accacttcct ctgtgagatc atcaacccgg
     3901 acacgctgga gccggtcgag atgggtgaga ccggcgaact ggtcatcacg actctgacca
     3961 aagaggccgt gccgatcatc cgctaccgga ccagggacat cacccgcctg gatccggagc
     4021 cgtgcgcgtg cgggcggacg acgatcaaga tgcaccgcgt cgttggccga agcgacgaca
     4081 tgctggtcgt ccgcggtacg aacgtcttcc caagccag
//
LOCUS       DAOSPL010000200         4114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21800_length_4114_cov_3.528948, whole genome shotgun sequence.
ACCESSION   DAOSPL010000200 DAOSPL010000000
VERSION     DAOSPL010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21800_length_4114_cov_3.528948"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLQ54_05040"
     CDS             complement(<1..280)
                     /locus_tag="PLQ54_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92655.1"
                     /translation="MRDSVAFRLLLVACLSVARAQSQDNPITNGGFEQLDASGRPVDW
                     APMAPFAIETADVRSGNRALRLTLKLPGEPDPGLNRAWAEGAGQRGSMI"
     gene            632..2185
                     /locus_tag="PLQ54_05045"
     CDS             632..2185
                     /locus_tag="PLQ54_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOS92656.1"
                     /translation="MSVSRSALGMTLMVALVVLCLPAAAEVSLPSVIGSNMVLQRDMR
                     CPIWGKATPGEIVRVTIDDQVKTTMASPAGRWMVRLDRLPAGGPHTLVVSGHNRITLT
                     NVMVGEVWVCSGQSNMQMSVAGVRDADIEIANANYPDIRLLTVPCVTADKPQDDFQGS
                     WTSCSPQTVPGFSAVGYFFGRKLHQELKVPVGLINTSWGGTPAESWTSRPSLEKHDVL
                     TPILDRWDKIVADYPTAIATYNNETVPAWEREAAKARAEGKEAPPKPGPPAGPDHPWR
                     PASLYNAMIHPLVPFGIRGAIWYQGESNADRAYQYRTLFPTMIKDWREAWGQGDFAFL
                     FVQLANFLPEKPEPGDSAWAELREAQLMTLKLKHTGMATIIDIGEAADIHPKNKQDVG
                     IRLALWPLAHCFGRDIECSGPLYRSMSRKGNAIHVRFDHVGKGLVAKGSPDLVGFAIA
                     GADRRFVWADAVIDGDEVVVSSRFIDKPVAVRYAWADNPRCNLFNLDGLPASPFRTDD
                     WPMVTANAR"
     gene            complement(2271..3431)
                     /locus_tag="PLQ54_05050"
     CDS             complement(2271..3431)
                     /locus_tag="PLQ54_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92657.1"
                     /translation="MDWLEHLVEARAVTAGPRHHFFGYYDKFPWSASGRYLLSLEGPF
                     VGRQPEPSEPVVVGMTDLEKGQWIPLDETLAWNWQQGTMLQWMPQAPDRLIIYNVREA
                     EGFASIIRDVHTGEIRRLPHPVYAISHDGAWAVTPSFARLAVTRPGYGYDGLPDPFET
                     QDAPEDDGIYRMELKTGKRELVVSLAQIASTGRDPTMEGAKHWFNHLQFCTDDSRFVF
                     LHRWRHGESWLTRMFTANPDGSEVYCVSDHGMVSHFDWRDADHILAWATRRGQGDHYF
                     LFRDQTGVVDLVGPDVLTVDGHCSYSPDRRWILTDTYPDSEHKRTLLIYEVATGRRVD
                     IGHFYSPPTLAGPWRCDLHPRWSRDGRQVCIDSAHEGTRQVYVLDVGEIVGV"
     gene            3548..>4114
                     /locus_tag="PLQ54_05055"
     CDS             3548..>4114
                     /locus_tag="PLQ54_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOS92658.1"
                     /translation="MILSCTTCSLRGRGRDEIEETLRYAPEAGYSAWGLGGPFTWEPG
                     LIQWLDTDQLRERMCMAGLPTLTEVWTPPIPTESPARAEEGARHVVLSARAAEKLGCD
                     RLVQTGGPRREGGLDQTLQGLRALDAALGSLPVRVCLEPHVGSQILYPDEYNVLMAEL
                     PTRRFGITVDTGHFHSAGVDWKALIRRFP"
BASE COUNT          685 a         1381 c         1358 g          690 t
ORIGIN      
        1 cgatcatgct tccgcgttga ccggccccct cagcccaggc ccggttcagg ccgggatcgg
       61 gctctcccgg aagcttgagc gtcaacctca gagcgcggtt gcccgatcgc acatcggccg
      121 tctcgatggc gaatggtgcc atgggagccc aatcgacggg ccgcccagag gcatccaact
      181 gctcgaatcc gccattcgtg atgggattgt cctggctctg ggctcgcgcg acactcaggc
      241 aggccacaag cagcaaacga aaggcgacag agtctcgcat gggtgaacgc cctccttgag
      301 gaatggccgt tcgctgccgt ccaacgccta ccctcgcgtg gcgtgcgggt tcactcctcc
      361 cgagcctgtc gggccttcac ggccggggtg cacccggcca aaggctgccg gaatccggca
      421 tggccgagct tggggtcgcc gcaacgcgcg ccgaccgcca gggcagggac cgtgccggca
      481 ggcggcggcc acgtctgtct cttgcacccc ggtcttgccg catccggcgc tcagcgtgtg
      541 cggtcgaagg gaacctggac tgtgaggctg aataccggtc tcggtcggcg tcgagcaggc
      601 cggttcgagt tggagtgcgg aggcgttcgg tatgtctgtg tcccgaagcg cactggggat
      661 gacgctcatg gtcgccctgg tggtgctctg cttgcccgct gcggccgagg tctctctgcc
      721 atcggtcatc gggagcaaca tggttctcca gcgtgatatg cgctgcccca tctggggcaa
      781 ggccacaccg ggcgagatcg tacgtgtcac catcgacgac caggtcaaga cgaccatggc
      841 gagccccgct ggccggtgga tggtacgcct ggaccgtctg ccggctggcg gtccacacac
      901 cttggtcgtg tccgggcaca accgcatcac cttgacgaat gtgatggtcg gcgaggtgtg
      961 ggtttgctcg ggccagtcaa acatgcagat gtcggtcgcc ggggtgcgcg acgccgacat
     1021 cgagatcgcc aacgcgaact atccggatat tcggttgctg acggtcccct gcgtcactgc
     1081 tgacaagcct caggatgact tccagggcag ctggacgagc tgctcgccgc agaccgtgcc
     1141 cggcttctcg gccgtcgggt acttcttcgg ccggaagcta caccaggaac tcaaagtgcc
     1201 cgtggggctc atcaacacct cgtggggtgg cactccggcc gagtcgtgga catcgcgccc
     1261 cagtctggag aagcatgacg tcctcactcc cattctcgac cgctgggaca agatcgtggc
     1321 ggactatcca accgccatcg ccacatacaa caacgagaca gtcccggcgt gggagaggga
     1381 ggccgccaag gccagggctg agggcaagga ggcaccgccc aagcccgggc caccggccgg
     1441 gcctgaccac ccatggcgac cggcatccct ctacaacgcc atgatccatc cgctggtgcc
     1501 gttcggcatt cgcggagcca tctggtacca gggggaatcg aacgccgacc gtgcctacca
     1561 gtaccggacg ctctttccca cgatgatcaa ggactggcgg gaagcttggg gccagggcga
     1621 cttcgcgttt ctgttcgtcc aactcgcgaa cttcctcccc gagaagcccg agccgggcga
     1681 cagcgcgtgg gccgagcttc gtgaggccca gctgatgacc ctgaagctca aacataccgg
     1741 catggccacc atcatcgata tcggtgaagc cgcggacatc caccctaaga acaagcagga
     1801 tgtggggata cgcttggcgc tctggccgct ggctcattgc ttcgggcggg acatcgagtg
     1861 ctccggcccg ctctaccggt cgatgagccg caaggggaat gcgatccacg tccgcttcga
     1921 ccatgtgggc aaaggcctgg tggccaaagg ctcgcccgat ctcgtgggat tcgccatcgc
     1981 gggtgccgac cgccgcttcg tgtgggcgga tgcggtcatc gatggcgacg aggtcgtcgt
     2041 ctcgagccgg ttcatcgaca agccagtggc tgtgcgctac gcgtgggccg acaacccccg
     2101 gtgtaacctg ttcaacctcg atggccttcc cgcatcaccg ttccgcaccg atgactggcc
     2161 gatggtgacc gcgaacgcgc ggtaaggccc gaggcgctcg ctccacacgc cgggccgggc
     2221 gggtaccgca cccgcccggc ccgggttctg cctgtacctc cgggagtggc ctagacaccg
     2281 acgatctccc ccacgtccag cacgtagacc tgacgggtgc cctcatgcgc cgagtcgatg
     2341 cacacctggc ggccgtcgcg gctccatcgc gggtgaaggt cgcaccgcca aggccccgcg
     2401 agcgtgggcg gcgagtagaa gtggccgatg tccacgcgtc ggcctgtggc gacctcgtag
     2461 atcagcagcg tccgcttgtg ctcgctgtcg gggtacgtgt ccgtgagaat ccaccggcgg
     2521 tccggcgagt agctgcagtg cccgtcgacg gtcaggacgt cagggccaac taggtccaca
     2581 acgccggtct ggtcccggaa caggaagtag tgatcgccct gcccgcggcg cgtggcccag
     2641 gcaaggatgt ggtccgcgtc gcgccagtca aagtgagaga ccatcccatg gtcgctcacg
     2701 cagtagacct cgctgccgtc cggattcgcc gtgaacattc gcgtcagcca gctctctcca
     2761 tgccgccagc ggtgcaggaa cacaaagcgt gagtcgtccg tgcagaactg gaggtggttg
     2821 aaccagtgct tggcaccctc catcgtcgga tcacggcccg tggaggcgat ctgcgccagg
     2881 gagacgacga gttcgcgctt ccccgtcttc agctccatgc ggtagatacc gtcgtcctcg
     2941 ggcgcgtcct gtgtctcgaa cgggtcgggc aggccatcat acccgtaacc cggccgcgtg
     3001 acggccaggc gagcgaagct gggggtcacc gcccacgccc catcgtggct gatggcgtag
     3061 acggggtgcg gcagccgacg gatctctccc gtgtggacgt cgcggatgat ggacgcgaag
     3121 ccctccgcct cgcgtacgtt gtagatgatg agccggtcgg gcgcctgagg catccactgg
     3181 agcatggtcc cctgctgcca gttccaggcg agggtctcgt cgaggggaat ccactggccc
     3241 ttctccaggt cggtcatgcc cacgacgacg ggctcggacg gttccggctg gcgccccacg
     3301 aacggaccct ccagagagag caggtagcgc cccgacgcac tccacgggaa cttgtcgtag
     3361 taaccgaaga agtggtgtcg cgggcccgcg gtgacggccc gcgcctcgac caggtgctcc
     3421 agccagtcca tcgtggcgtt ccccccagac ggcctctgtt cgcatcgtcg acaggacccc
     3481 tcgccgcgag tgcggaacaa ccataggcgc ggatgcgaga acgcgaatcc catcgggggg
     3541 agtttccgtg atcctgtcgt gcaccacctg ctcgcttcgg ggacgaggtc gggatgagat
     3601 cgaagagaca ctccgctacg cccccgaggc gggatactca gcctggggtc tcggcgggcc
     3661 cttcacatgg gagcctgggc tcatccagtg gctcgacacc gaccaactgc gcgagcggat
     3721 gtgcatggcc ggtttgccaa ccctcaccga ggtgtggacg ccccccatcc ccaccgagtc
     3781 gcctgctcgg gcggaggagg gtgcgcgcca tgtggtcctg tctgctcggg ccgccgagaa
     3841 gttaggctgc gatcggctag tccagaccgg cggcccacgg cgcgaggggg gtctggacca
     3901 gacgctgcag gggctccgcg ccctcgacgc ggcgctgggg agcctccccg ttcgcgtgtg
     3961 cctcgagcca cacgtgggct ctcagatact ctaccccgac gagtacaacg tgctcatggc
     4021 cgaactgccc acgcggcgat tcggcatcac cgtggatacc ggccacttcc actcggccgg
     4081 tgtggactgg aaggccctca tacgccgctt cccg
//
LOCUS       DAOSPL010000201         4114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21801_length_4114_cov_3.376447, whole genome shotgun sequence.
ACCESSION   DAOSPL010000201 DAOSPL010000000
VERSION     DAOSPL010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21801_length_4114_cov_3.376447"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PLQ54_05060"
     CDS             <1..475
                     /locus_tag="PLQ54_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92659.1"
                     /translation="RLIEFAGCGVSPSGRIQYIGRGENADDPRFGVPREVQCLISACI
                     LMPITAQRAAGHMDTAYSPVQYEDLDYCYRLKELGYSCWVLPQVEMYHYEHITTAGSA
                     SINFRYVTIRNGMRFKQRWQHVFSRENGPRDEDTTWKEIDRYRLPDGETPNDAAC"
     gene            561..821
                     /locus_tag="PLQ54_05065"
     CDS             561..821
                     /locus_tag="PLQ54_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOS92660.1"
                     /translation="MATPDELIPPLKQLIVDKLFLKVDPAEMDADKPLMDTYGIDSVS
                     LFEIVVGLEEEYGIVLADDEFDIELFRSVRSIAEFAASKLDS"
     gene            826..1656
                     /locus_tag="PLQ54_05070"
     CDS             826..1656
                     /locus_tag="PLQ54_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92661.1"
                     /translation="MPSCAEVHSFVAVEGRRLYVAEHRPEGAIRSAALFCLPIHEEEK
                     SVYRPMVDAARLAAERGCMSLRFHYTGTGDSSGSPLDMTVDTLVRDTLDAAQILRQRS
                     GVEPVTLVGVRLGAAIALLAAERIGACAGVLLVEPVPSGRLYLSRARTRKRVRRMVTA
                     AEGGEGVADSLGAPDQPPAGGVFDFDGHPIAPGLENSLAALDLAQRTAQLPVRVEVIE
                     IAPRDRISGSVERLGSVLEARGARVRVRALVAEPFWNNLGVVHIPEVCQAIAGFVAGD
                     "
     gene            1799..2641
                     /locus_tag="PLQ54_05075"
     CDS             1799..2641
                     /locus_tag="PLQ54_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOS92662.1"
                     /translation="MQETLEFVSNGLAVRGIVHRPEGRTPRAGVLLLHGWGGTRSGPH
                     RILHSAGERLAGEGFVCLRFDFRGRGDSEGEVLDHDLNTMIEDAKCAAEYAHVALGLD
                     TLYLVGICSGGEVAVGAMHDGLKVAGAALWSAPVFSADATLSRRARKSASYLRDYAAK
                     LFRLETWRKLISGRIRFGVIGRVLLGGSVHQKGEGERKELSASERLRGGADSVLLIYG
                     TADPIAEEAIDRYTALFERSGARVSLHRIVGANHGFYSWAWHQEVVERTAAWMLAEAA
                     LTAP"
     gene            2638..3750
                     /locus_tag="PLQ54_05080"
     CDS             2638..3750
                     /locus_tag="PLQ54_05080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS92663.1"
                     /translation="MIRVLCVNHCSGISGAEVSLLTLLSALDRSRYEPHVAAPPGGLL
                     DHCAHLGVPVHPISLPWVRRPRGIGDALTAFSHAHVGRQSLKRLADELDPGLIHANST
                     VAQVYASALHPGRAVIWHARDMQPLGVVGPYLSSHADALIAVSRAALRYHYSRGCRPR
                     LTRAVYNGVDCGQFHPGVDGRPVRQELGVPEWATVFGCIGQAVSWKRHQDFIGAARCL
                     EHLGWHFVLVRTDPFGHGEPLKEIIGPRLHILEGHGNVPALIAALDVLVLPSAYEPFG
                     RTLIEAMALAKPVIATDTGGPPEVVVPGHTGLIVPTGDVAALAAAMERLGGDPGLRRR
                     MGEAGHDRVLSSFTAEAHARAVEAVYEEVLAARASS"
     gene            3747..>4114
                     /locus_tag="PLQ54_05085"
     CDS             3747..>4114
                     /locus_tag="PLQ54_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92664.1"
                     /translation="MRIGIDATVIYGRYTGVARAVVGLIRGLAEVDDENDYVLYCGRD
                     IPPLGELPANFRWRTRPMDVASRLRVVYWQQSRLDADAREDGVDVLHCPAYVAPMRGR
                     TKTVLSVYDAHVFTHPQYCS"
BASE COUNT          629 a         1368 c         1396 g          721 t
ORIGIN      
        1 gcggctcatc gagttcgcag ggtgtggcgt gtcgccctcg ggccggatcc agtacatcgg
       61 gcgcggcgag aacgctgacg acccccggtt cggtgtgccc cgcgaggtcc agtgcctcat
      121 cagcgcgtgc atcctgatgc ccatcactgc ccagcgggct gccggtcaca tggataccgc
      181 ctacagcccg gtgcagtatg aggatctcga ctactgctac cggctcaagg agctcggcta
      241 ctcgtgctgg gttctgcctc aggtcgagat gtaccactat gagcacatca ccacggccgg
      301 atcggcttcg atcaacttcc ggtatgtgac tatccggaat ggaatgcgtt tcaagcaacg
      361 ctggcagcat gtgttctcgc gggagaacgg cccccgcgac gaggacacga cctggaagga
      421 gatcgaccgt taccggctgc ccgatggcga gacaccgaac gacgctgcgt gctagccggc
      481 gccaccggga ggtcacccga gcgggttcat cgctttaccc cggggccgtt cgcctcgggg
      541 catgatgggg agagatggtc atggccacac cggatgaact gattccgccg ctgaaacagc
      601 tcatcgtcga caagctgttc ctcaaggtgg atccggcaga gatggacgcg gacaagccgc
      661 tcatggacac ctacggcatc gactcagtca gtctcttcga gatcgtggtg ggcctcgagg
      721 aagagtatgg catcgtgctt gccgacgacg agttcgacat cgagctcttc cggtcggttc
      781 gttctattgc cgagtttgcc gcatcgaaac tggattcgta gccccatgcc atcgtgcgcc
      841 gaggttcaca gctttgtggc ggtggaaggc cgtcgcctct acgtggccga gcatcgtccc
      901 gagggcgcca tacggtcggc ggcgctcttc tgtctaccga tccacgagga ggagaagtcg
      961 gtctatcgtc ccatggtaga cgcggctcga ctcgccgctg agcgcggatg catgagcctg
     1021 cggttccact acaccggtac gggcgacagc agcggcagcc ccctcgatat gaccgtcgac
     1081 acactggtgc gggacaccct cgatgcggcc cagatcctcc ggcagcgcag cggcgtggag
     1141 cccgtcacac tggtcggcgt ccgattgggt gctgccatcg cgctgttggc cgctgagcgg
     1201 atcggtgcct gcgccggcgt gctgctcgtt gagccggtgc cgagcggccg gctgtacctc
     1261 tcgcgtgctc gcacgcgcaa gcgcgttcgg cgcatggtca ccgccgcgga gggaggggag
     1321 ggtgtggctg actcgctcgg cgccccggat caacctccgg ccggcggagt gttcgacttc
     1381 gacgggcatc ccatcgctcc cggcctcgag aactcgctgg cggcgttgga tctggcacag
     1441 cgaaccgctc agctgccggt gcgtgtcgag gtgattgaga tcgcgcctcg cgaccgcatc
     1501 tcgggatccg tcgagagact cggatcggtc ctcgaagccc gaggcgcccg cgtgagggtt
     1561 cgggccctag tggcggagcc attctggaac aacctggggg tcgtgcacat acccgaggtg
     1621 tgccaggcca tcgcgggttt cgtggcgggc gattgacact ccgctgggtg gccccagcgg
     1681 aggacggggc ggcagcgggc tgaggcccgg agcgcgggtg agcacgctcg tgcaggcccg
     1741 ccgccaccga tcgggaatcc tcgtcctgtt acgggcagcc gctggaaggg tgcgggccat
     1801 gcaagagacc ctcgagttcg tcagcaacgg cttggccgta cggggtatcg tgcaccggcc
     1861 cgagggtcgc acgcctcgag cgggggtact tctccttcac ggctggggcg gaacgcggtc
     1921 gggtcctcac cgcatcctcc actcggccgg ggagcgcctg gcaggcgaag gtttcgtctg
     1981 cctccggttt gacttccggg gccggggaga cagcgaaggc gaagttctgg accatgacct
     2041 gaacaccatg atcgaggacg ccaagtgcgc cgccgagtat gcccacgtgg cgctgggact
     2101 ggacacgctg tacctggtcg gcatctgttc gggcggtgag gtcgccgtgg gcgcgatgca
     2161 tgacggcctg aaggtcgccg gcgcagccct gtggtcggcc cccgtgttct cggccgacgc
     2221 caccctttcc cggcgcgcgc ggaaatccgc gtcctacctg cgtgactacg cggccaagct
     2281 cttccgcctt gagacctggc gcaaactcat ctcggggcgc attcgcttcg gggtgatcgg
     2341 ccgggttctc ctcggcgggt cagttcacca gaagggcgag ggcgagcgga aggaactctc
     2401 ggccagtgag cggctccgcg gcggggccga ctcggtgctc ctgatatatg gcaccgccga
     2461 ccccattgcc gaggaggcca tcgaccgcta caccgcgctc tttgagcgct cgggagcccg
     2521 ggtcagcctc caccgcatcg tgggggccaa ccacggattc tactcatggg cgtggcacca
     2581 agaggtcgtt gagcgcacag cggcgtggat gttggccgaa gcggccctca cagcgccatg
     2641 atccgcgtgc tctgcgtcaa ccactgcagc ggcatctcgg gtgccgaggt gtcgctgctg
     2701 acactgctct cagctctcga ccggtcgcgc tatgagcccc acgtggcagc cccccccggt
     2761 gggcttctcg accactgcgc ccacctgggc gttcccgtcc accccatttc cctcccctgg
     2821 gtccgtaggc cgcgagggat cggtgatgcc ctcacggcat tctcgcatgc tcacgttggc
     2881 cgccagagcc tgaagcgcct ggctgacgag ctcgatcccg gcctcatcca tgccaacagc
     2941 accgtggcgc aggtctacgc cagcgcactg cacccgggac gcgcggttat ctggcatgca
     3001 cgcgatatgc agccactggg ggtggtgggg ccctatctct ccagccatgc cgatgccctg
     3061 attgccgtgt cgcgggccgc gctccggtac cactatagcc gtggctgccg tccccggttg
     3121 acgcgggccg tctacaatgg cgtcgactgc ggccagttcc accccggtgt cgacgggcgg
     3181 cccgtccggc aggagctcgg agtgcccgaa tgggctacgg tgttcgggtg tatcggccag
     3241 gccgtgtcgt ggaaacgcca ccaggacttc atcggcgccg cccggtgcct cgagcacctg
     3301 ggttggcact tcgtgctggt acgtaccgac ccattcggtc acggggagcc gctgaaggag
     3361 atcatcggac cgcggttgca tatcctggaa ggtcatggga atgtgcctgc cctgatcgcc
     3421 gctctggacg tcctggtcct gccgtcggcc tacgagcctt tcggccgcac gctgatcgag
     3481 gccatggcgc tggcgaagcc cgtgatcgcg acggacacgg gggggccgcc cgaagtggtt
     3541 gtgccagggc acacaggcct gatcgtgccc acaggcgatg tggcagccct ggcggcggcc
     3601 atggagcgcc tgggcggcga ccccgggcta cgccgccgga tgggcgaggc gggccatgac
     3661 cgtgtcctgt cctccttcac cgccgaggcc cacgcccgag cggtggaggc ggtctacgag
     3721 gaggtcctgg ccgccagggc aagctcttga ggatcggaat cgacgccaca gtcatctacg
     3781 gccgctatac cggcgtggcc cgtgccgtgg tcggactcat ccgcgggctg gccgaggtcg
     3841 acgacgaaaa cgactacgtc ctctactgcg gccgcgacat cccaccgctc ggtgagctgc
     3901 cagccaactt ccgctggcgc acccggccga tggatgtggc ctcacgcctg cgtgtggtct
     3961 actggcagca gagccggctc gacgccgatg cccgcgagga cggggtcgac gtgctccact
     4021 gccccgccta tgtcgccccc atgcggggca ggacgaaaac ggttctctcg gtctatgacg
     4081 cccatgtctt tacccacccc cagtattgct cgtg
//
LOCUS       DAOSPL010000202         4114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21804_length_4114_cov_2.920916, whole genome shotgun sequence.
ACCESSION   DAOSPL010000202 DAOSPL010000000
VERSION     DAOSPL010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21804_length_4114_cov_2.920916"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..918)
                     /locus_tag="PLQ54_05090"
     CDS             complement(133..918)
                     /locus_tag="PLQ54_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS92665.1"
                     /translation="MKKGICLGCLPPDLDDLGRMKLARDAGFDGVEINTPASDGQAED
                     LRAMAEQAGIEIHSVMDSLHWECPLSDPDDAVRRRGMESLKASIRAAKITGATAVLVV
                     PGVVNDTVSYEQAWQRSMAGLKELAPYAAEHRVPLCVENVWNRFLLSPREMVEFIDTI
                     GSPFVQAYFDVGNIVLYGNGDQWIRSLGPRVKKIHLKDFSRSEMRFVYLFQGDVPWQR
                     VMHALASIGYGDYLTAEMGPYRGCPGITAFDISRHIDHLFGMV"
     gene            complement(1015..1494)
                     /locus_tag="PLQ54_05095"
     CDS             complement(1015..1494)
                     /locus_tag="PLQ54_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92666.1"
                     /translation="MDAQPGTDEPDHPDVADLADAPENHRRGIRTTLVMVDQLLCRVD
                     QWAGGYAARSALIDERNTLSREQCVQLRALTEELRGLIARAAADLHLEPETPDAAQSI
                     WSECLSLRVGVTELGAHHLRRYGEVPPRLADYADALAEVLADGLSRLSDAATGGRLV"
     gene            complement(1502..2698)
                     /locus_tag="PLQ54_05100"
     CDS             complement(1502..2698)
                     /locus_tag="PLQ54_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006341662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOS92667.1"
                     /translation="MSDTFTTAAVWFALALGATLLARHLRASIALVEICVGTFAGALA
                     QHYGGPEILGSDQEWIRFLAASGAVLLTFLAGAELEPAVLRSKAREVLIVGAVGFVAP
                     FLGCAAAARFLLHWDPRASWLAGVALSTTSMAVVYAVMLETGLNRTEFGKGILGACFV
                     NDLGTVLALGIIFAPFTMRTAIFGGVTVVTLAILPGATRLLTRIYGHRTAAVRIKWVL
                     LVLFGLGALALWAGSEAVLPAYVAGMLLAGQAAEDHHWVRRVRTLTVGFLTPFYFLRA
                     GSLVSLPAIAAGALPFVVLLGGKVASKIIGLNPVIRQFRSGRGESWYYTLLMSTGLTF
                     GTISALYGLTHGIVTRAQYSLLVAVVIASAVIPTLIANAVFLPRHLMLPSEPAVAEEP
                     EGPESE"
     regulatory      complement(2789..2850)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(2882..3724)
                     /locus_tag="PLQ54_05105"
     CDS             complement(2882..3724)
                     /locus_tag="PLQ54_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006884201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOS92668.1"
                     /translation="MRFGAPVFVDSRDPAVLVEAHKKKGFTAAYCPGGITAQDPDARL
                     DEVKQAFAEADIVIAEVGAWCNCLDPNPEARERNITYVIGQLALAEKIGARCCVDYSG
                     SFSPDHPAGPHPDDLSERAFELVVANTQRIIDAVDPQSTYFTLEMMQWAWPDSPDAYL
                     DLIEAVDRDAFAVHFDPVNLVRSPRHYYENGKLIEECVLKLGDFIRSVHGKDCIMSDQ
                     YLVHITECPAGTGRLDYATLLRELDRLPQDVPLMLEHLASEDEYDQAAAYVRSVAEEV
                     GVEL"
     gene            complement(3781..>4114)
                     /locus_tag="PLQ54_05110"
     CDS             complement(3781..>4114)
                     /locus_tag="PLQ54_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92669.1"
                     /translation="NIPLPATRRGWLDLPRVRAGRGLRLKFGTVLTGPSFDGVRMSVL
                     VDGRELWAQVQPGQNLPKAHAIDLTDWAGRRVRLTLRVDALGNNGADWAHWLRPVVVV
                     TGPMALNR"
BASE COUNT          675 a         1296 c         1504 g          639 t
ORIGIN      
        1 cggatggcgc ccttcccgct gcgcgggccc cttccattcg gcccctcctc ggttgctcag
       61 aggcctgccg gaacccggcg caggctgatg ggacgctatc cgagccggcc gtcagcgggg
      121 cggagacgcg ctctacacca tgccgaacag gtgatcgatg tgccgcgaga tgtcgaaggc
      181 ggtgataccc ggacagccgc gatagggacc catctcggcg gtgaggtagt cgccgtaacc
      241 gatgctggct agggcgtgca tgacgcgctg ccagggaacg tcgccctgga agaggtagac
      301 gaagcgcatc tcgctgcggc tgaagtcttt caagtggatc ttcttgactc ggggccccag
      361 ggaccggatc cactggtcac cattgccgta gaggacgatg ttgcccacgt cgaagtaggc
      421 ctggacgaac ggggagccga tggtgtcgat gaactcgacc atctcgcggg gagaaaggag
      481 gaacctgttc cagacattct cgacgcagag gggcacccgg tgctcggcgg cgtagggtgc
      541 gagctccttg aggccagcca tggagcgctg ccaggcttgt tcgtagctca cggtgtcgtt
      601 gaccacgcct gggaccacga ggacggcggt ggctccggtg atcttggcgg cgcggatgct
      661 ggccttgagg ctctccatgc cgcggcggcg gacggcgtca tcggggtcgg atagggggca
      721 ttcccagtgc agggagtcca tgacgctgtg gatctcgata ccggcctgct ctgccatggc
      781 ccgcaggtcc tcggcctggc cgtcgctggc aggcgtgttg atctcgacgc cgtcgaaccc
      841 ggcgtcgcgg gcgagtttca tccggccgag gtcgtcgagg tccggcggga ggcaacccag
      901 gcatatcccc ttcttcacag gcggctcggt cctctcacag gccacgaagc gccgcgggga
      961 cgctcggacc ccatggcagt gtcgcgcgga ttctacgggg tgccgggagg ggcgtcaaac
     1021 gaggcgcccg cctgttgccg catccgagag ccgactgagt ccgtccgcaa gcacctctgc
     1081 cagagcgtcc gcgtagtcgg ccaatcgcgg cgggacctcg ccatagcgcc ggaggtggtg
     1141 tgcgccgagc tcggtgacgc cgacgcggag ggaaaggcac tcgctccata tggactgggc
     1201 ggcgtccggc gtttcgggtt cgaggtgcag gtccgcggcg gcgcgggcga tgaggccgcg
     1261 gagctcctcg gtcagcgcgc gcagctggac gcactgctct cgagacagcg tgttccgttc
     1321 gtcgatcagt gcggatcggg cggcgtaccc gccggcccac tggtcgacgc ggcacagtag
     1381 ctggtcgacc atgacgagcg tcgtccggat accgcgacgg tggttctcgg gagcgtcggc
     1441 gaggtcggcc acgtcggggt gatcgggctc gtccgtgcct ggttgcgcgt ccatggacgg
     1501 atcactcgct ctctggcccc tccggctctt cggcgacggc gggctccgac ggcagcatca
     1561 ggtgacgggg caggaacacc gcgttcgcga tcagggtcgg tatgaccgcg ctggcgatga
     1621 ccacggccac caggagggag tattgcgccc gggtgacgat gccgtgagtc aggccgtaga
     1681 gggccgagat ggtgccgaag gtcaggccgg tcgacatcag cagcgtgtag taccagctct
     1741 ccccacggcc gctccggaac tgccggatga ccgggttgag gccgatgatc ttcgaggcga
     1801 ctttgccccc gagcaggacc acgaagggca gtgcgcccgc ggcgatggca ggcagggaga
     1861 ccagcgaccc ggcgcgcagg aagtagaagg gcgtgaggaa gccgacggtc agggtcctca
     1921 cccggcggac ccagtggtga tcctcagccg cctggcctgc caggagcatt cccgccacgt
     1981 aggcgggcag cacggcctca ctcccggccc agagggctag agctccgagg ccgaacaaca
     2041 cgagcagcac ccatttgatg cggaccgcgg cggtgcggtg gccgtagatc cgggtgagca
     2101 ggcgggtcgc accgggcagg atggccaggg tgaccacggt cactccgccg aagatggccg
     2161 tgcgcatggt gaagggcgcg aagatgatgc ccagtgccaa gacggtgccc aggtcgttga
     2221 cgaagcacgc gccgaggatg cccttgccga actcggtgcg gttgaggccc gtctcgagca
     2281 tcactgcgta gacgacggcc atggatgtgg tcgagagcgc cacaccggcc agccagctgg
     2341 ctcgggggtc ccagtgcagc aggaaccggg cggccgccgc gcaaccgagg aagggcgcga
     2401 cgaacccgac cgcgccgacg atgagcacct cgcgggcctt ggagcggagc acggcgggct
     2461 cgagttcggc cccggcgagg aaggtcagca gcacggcgcc ggatgcggcg aggaagcgga
     2521 tccactcctg atcgctgccg aggatctcgg ggccgccgta gtgctgggcc agtgcaccgg
     2581 cgaaggtgcc gacacagatc tcgaccagtg ctatcgaggc gcgaagatga cgagcgagga
     2641 gtgttgcgcc cagggcgagg gcaaaccata cagcggcagt ggtgaaggtg tcggacacgg
     2701 gcggagtcgc cccctgcata cgccaaggct tgcgcgcgca catgggccgc gcaacgcccg
     2761 gcaaaggctg tcctgccgcg aagcggcacg agcggcagga gtcatcagcc ccgcaaggcg
     2821 gttgaggggg actccatccc cgtatgccgc gattgtaccg ccagtcggcc agggccggca
     2881 actacagctc gacgccgacc tcctcggcca ccgaccgtac gtaggcggcg gcctggtcgt
     2941 actcgtcctc ggaggccaag tgctcgagca tcaacggcac atcctggggg agccggtcga
     3001 gctcgcgcag gagcgtcgcg tagtcgagcc gcccggtacc ggcggggcat tcggtgatgt
     3061 gcacgaggta ctggtcggac atgatgcagt ccttgccgtg cacgctccga atgaagtcgc
     3121 cgagtttgag cacacactcc tcgatcagct tgccgttctc gtagtagtgc cggggactgc
     3181 ggacgaggtt cacggggtcg aagtggacgg cgaaggcatc gcgatcgacg gcctcgatga
     3241 ggtcgaggta ggcatccggc gagtcgggcc aagcccattg catcatctcg agggtgaagt
     3301 aggtgctctg tgggtcgacg gcatcgatga tgcgctgggt gttggcgacg accagctcga
     3361 aggcgcgttc ggagaggtcg tccgggtgcg gcccggccgg gtggtcgggg ctgaaggagc
     3421 cggagtagtc gacgcagcag cgtgcgccga tcttctcggc gagcgccagc tggccgatga
     3481 cataggtgat gttccgctcg cgggcctctg ggttcgggtc gaggcagttg caccaggcgc
     3541 cgacctcggc gatgacgatg tcggcttcgg caaaggcctg cttcacctca tccagtcgcg
     3601 cgtcggggtc ctgggccgtg attccgccgg ggcagtaggc agcggtgaaa cctttcttct
     3661 tgtgcgcctc caccagcacg gcgggatctc tggagtccac gaacacggga gccccgaatc
     3721 gcatggtgtc cacctcctgt gacttggcag gagggtggtt cgccgctggc cacggaggtc
     3781 ctaccggttc agagccatcg gtcctgttac cacaaccact ggacgcagcc agtgcgccca
     3841 gtcggcgccg ttgttgccga gggcgtccac gcgcagcgtt agtcgcacgc gcctgcccgc
     3901 ccagtctgtc aggtcgattg cgtgggcctt cggtaggttc tggcctggct gcacctgggc
     3961 ccagagctcc cggccgtcaa cgagcaccga catgcgaacg ccgtcgaagc tcggcccggt
     4021 gagcacggtg ccaaacttca gccgcagccc ccggccggct cgcacgcgcg gcaggtccag
     4081 ccagccgcgc cgggtagctg gcaggggaat gttg
//
LOCUS       DAOSPL010000203         4106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21857_length_4106_cov_3.061219, whole genome shotgun sequence.
ACCESSION   DAOSPL010000203 DAOSPL010000000
VERSION     DAOSPL010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21857_length_4106_cov_3.061219"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PLQ54_05115"
     CDS             <1..653
                     /locus_tag="PLQ54_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOS92670.1"
                     /translation="DSAENVRALEWLCALVERQGGSAQVDAFSAGFGEYASSQQEYFA
                     GKVAMVESGQWWPSFTERFAREMDYGVCALPYPADRPDLRNTVPFGGNFACIPAGSRH
                     PDLAWEFLAWTQTRVAQAHFARVMHGVPNLKQFLELPELTTGSRENEAFGVVCSLAGG
                     PNGRAFPVTPANTLFSRELELAVDFATHGTKTPAQAMHDVTVRVQREIDQILEAGR"
     gene            650..1543
                     /locus_tag="PLQ54_05120"
     CDS             650..1543
                     /locus_tag="PLQ54_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010493320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOS92671.1"
                     /translation="MSLADRRRLVRGLLFVSPWLVGFVGLQLYPMLLSLYLSFCRYNT
                     LTPPRFVGIENYRTMLFADEYVWTSIGNTLFMMLELPASVVLGIGTALLLNRAARAIG
                     LFRTLYCLPYVLPSVSAGLLWLWVLNSDQGLVNALLGLLHIPGPSWLKDAQWAKPGFM
                     LMDLWGIGGSTLIYLAALQGVPQHLHEAAELDGAGPWARFRHVTLPAVSPTVFFLVVT
                     GALGVLQYFAQTFVMTKGQPENSTLFFGLYLFQNAFEYFRMGFACAMAWLLFLVALLA
                     TYLIFRTSARWVYYEAQEGAR"
     gene            1540..2376
                     /locus_tag="PLQ54_05125"
     CDS             1540..2376
                     /locus_tag="PLQ54_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOS92672.1"
                     /translation="MSRSLARLAAGAALVLFAVLFILPLLWMVSTSLQPEGQVLSGTG
                     NALTHLIPRRLAPVNYVKALQTLHFVPYLLNTLYVCVLVVAGTLASCTVVAYGFSRLR
                     WPGRDTLFSIMLATIMLPPAVLLLPQFVVFRSLGWVGTYKPLIVPAFLGNAFFIFLLR
                     QFFLGIPEELTDAARIEGASEPQILWHVIAPLAKPALVTVALLCFVWSWTDFMGPLVY
                     LSDDRMYTLTVGLASFLDRHRADWSGLMAGTTLVTTPLAVLFLLGQRFFVRGIQMTGL
                     KG"
     gene            2418..>4106
                     /locus_tag="PLQ54_05130"
     CDS             2418..>4106
                     /locus_tag="PLQ54_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS92673.1"
                     /translation="MLTRLLVRNFRRLEEADIGLDAVTMLAGPNDSGKTAALQALCLW
                     ALGLRRWRERRSGHAAPGKRLGVPVNRRDLIAVPLPHTDLLWHDLRLRDTSRATQNVL
                     IEVTVSGATRGVLWECGLEFDYANEELFYVRPLRLGGGRGAERMEVPDEASDVRVALL
                     PPMAGLAAVEPRWEPGRTNVLIGEGQTAQVLRNLCYQVSQAGEGLWDRLAAHVRDLFG
                     VELLRPRHLPERGEITMQYRQGRTVFDLSSSGSGMLQTVLLLSYLYSNPGAVLLVDEP
                     DAHLEVLRQRQTLDVLVEAARANEVQLIIATHSAALLEEAVGRHHVVVLAEGHPSSLE
                     DQGPRVMEALPHVGCGQYLLARWTGWVLYLETPRALTILRAFARRLGHRALSALERPF
                     VRYVGAEPEQAIGHFRRLQCVLPGLIGVGLFATPGRDLPSTGDGLRTLQWTRAEAECY
                     LCTEAALVAWARGAQRDLFGEHRAEAMGEAIRETLSVLRALGKSDPFGGRLRVSEEFL
                     PPVFDAYRRLTGGEGRTLSKATYPELVEFLPQGAIDPEVVEKLDAIAQVADGARP"
BASE COUNT          576 a         1345 c         1366 g          819 t
ORIGIN      
        1 ccgacagcgc cgagaacgtc cgggcgctcg aatggttgtg tgccctggtc gagcgtcagg
       61 gagggagcgc ccaggtcgat gcgttctccg ccggcttcgg cgagtacgct agctcccagc
      121 aggagtactt cgccgggaag gtggcgatgg tggagtcggg ccagtggtgg ccatcgttca
      181 cggagcgttt cgcgcgggag atggactacg gcgtgtgtgc cctcccctac ccagcggatc
      241 gcccggatct gcggaacacg gtgccctttg gtggcaactt cgcgtgtatc cccgcgggct
      301 cgcggcatcc ggacctggcc tgggagttcc tggcatggac gcagacccgg gtggcccagg
      361 cgcactttgc ccgcgtcatg cacggcgtgc cgaatctcaa gcagtttctc gaactgcctg
      421 aactgaccac gggctcgcgg gagaacgagg cttttggagt cgtctgctca ctcgccggtg
      481 gtcccaacgg ccgggccttt ccggtcacgc cggcaaacac gctgttttcc cgagagctgg
      541 agcttgcggt tgacttcgca acccatggca ccaagacacc ggcgcaggcc atgcacgacg
      601 tcacggtccg cgtccagcgc gagattgacc agatcctgga ggccggacga tgagcttggc
      661 tgaccgccga cggctcgtgc gggggttgct gtttgtctcc ccgtggctgg ttgggttcgt
      721 gggtctgcag ctctatccga tgctgctctc gctctacctg agcttctgcc ggtacaacac
      781 gctgacaccg ccacgttttg tcgggatcga gaactaccgc accatgttgt tcgcggatga
      841 gtatgtctgg acgtccatcg ggaacacact cttcatgatg ctggagctgc ccgccagcgt
      901 ggtgctcggg atcggcacgg ccctgttgct caaccgcgcc gcgagggcga tcggcctgtt
      961 ccggacgctc tactgtcttc cgtatgtcct cccctcggtc agcgccggcc tgctgtggct
     1021 gtgggtgctg aactctgacc aaggtctggt gaatgccttg ctgggtctgc tgcacatccc
     1081 cgggccgagc tggctcaagg acgcccagtg ggcgaagccg gggttcatgc tcatggacct
     1141 atggggcatt gggggcagca cactcattta cctggcggcg ctccagggcg tgcctcagca
     1201 cctgcacgag gctgccgagc tcgatggtgc agggccctgg gcgcgattcc gccatgtcac
     1261 gcttcccgct gtctcgccca cagtcttctt cctcgtggtg acaggggcgc tgggcgtgct
     1321 gcagtatttc gcgcagacct tcgtcatgac caagggccaa cccgagaact caaccttgtt
     1381 cttcgggctc tacctgttcc agaacgcctt cgagtatttc cgcatgggct ttgcctgtgc
     1441 gatggcctgg ctgctgttcc ttgtggcgct cctggccacg tacctcatct tccggacctc
     1501 ggcccggtgg gtctactacg aggcacagga gggggcccgg tgagccgatc actggcgcgt
     1561 ctggcggccg gcgccgcgct tgtcctgttc gccgtgctct tcattctgcc gctgctgtgg
     1621 atggtcagca catcgctcca accagagggc caggtcctgt cggggaccgg caacgcgctg
     1681 acgcatctga tcccccgcag gctggcgccg gtcaactacg tgaaggccct gcagacgctg
     1741 cacttcgttc cctacttgct gaacacgctc tacgtgtgcg tgctggtcgt ggcgggcacg
     1801 ctggcttcgt gtaccgtggt ggcctatgga ttctcgcggc tgcggtggcc aggacgcgac
     1861 acgctgttct cgatcatgct cgcgacgatc atgctaccac cggctgtgct gcttctgccg
     1921 cagttcgtgg tgttccgaag cttgggttgg gttggcacgt acaagcccct catcgtccct
     1981 gcgtttctgg gcaatgcgtt cttcatcttc ctgctgcggc agttcttcct cgggatccct
     2041 gaggaactca ccgatgccgc tcgtatcgag ggagcgtcgg agccgcagat cctatggcat
     2101 gtcattgctc cgctggcgaa gccggctctc gtcaccgtgg cgctgctctg ctttgtttgg
     2161 tcgtggacgg acttcatggg gccgctggtg tacctctcag acgaccgcat gtacacgctt
     2221 accgtgggtc tggcgtcgtt cctcgaccgt catcgggccg actggagtgg cctgatggcg
     2281 ggcacgacgc tggtgacgac gccgttggcc gtcctgttcc tcctcggcca gcgcttcttc
     2341 gtgcgaggga ttcagatgac cgggctgaag gggtagggcg accgcagacc gcacacttcc
     2401 ggcggaggta gctcacggtg ctcacacgac tcctcgtccg taacttcagg cggctggagg
     2461 aagccgacat cggcctggat gcggtcacca tgctcgccgg ccccaacgac tccggcaaga
     2521 ccgcagcgct ccaggcgctg tgcctctggg ccctcggcct ccgtcgttgg cgagagaggc
     2581 gctcggggca cgccgccccg gggaagcgcc tcggcgtacc ggtcaaccga cgcgatctca
     2641 tcgccgttcc cttgccgcac acggacctgc tctggcatga tcttcgcctg cgtgacactt
     2701 cgcgggccac tcagaacgtg cttatcgagg tcacggtctc gggtgccact cggggcgtcc
     2761 tctgggagtg cggtctcgag ttcgactacg ccaacgagga gctcttctac gtccgtccac
     2821 tccgcctggg cggcggccgc ggcgcggaac ggatggaggt cccggacgag gcctccgacg
     2881 tacgtgtcgc ccttctgccc cccatggccg ggctggccgc cgtcgaaccg aggtgggaac
     2941 ccgggcgcac caacgtgctc atcggtgaag ggcagaccgc tcaagtcctc cgcaacctgt
     3001 gctaccaggt ttcccaggcc ggtgaaggtc tatgggatcg gctcgcggct catgttcgtg
     3061 atctgtttgg cgtcgagctg ctgcgtccgc ggcacctccc ggagcgcggg gagatcacga
     3121 tgcagtaccg gcaaggccgc acggtattcg acctgagttc gtcggggagc ggcatgcttc
     3181 agaccgttct gcttctgagc tacctctact cgaaccccgg ggccgtgctg ctcgtcgacg
     3241 aaccggatgc ccacctggag gtgctccgcc agcgccagac cctcgacgtg ctcgtggagg
     3301 cagcgcgcgc caacgaagtg cagctcatca tcgccaccca ctcggcggcc cttcttgagg
     3361 aggcggtggg tcggcaccat gttgtcgtcc tcgccgaggg ccacccgagc tctctggagg
     3421 accaggggcc acgagtgatg gaggcattgc cccatgtggg ctgcggccag tacctgctgg
     3481 cccgctggac cggttgggtg ctctatttgg agacgccgcg cgcactcacg atcctccgcg
     3541 cgttcgcccg gcgattgggg caccgtgccc tatcggcact ggagaggcct ttcgtccggt
     3601 atgtgggcgc ggagccggag caagcgatcg ggcatttccg ccggctccag tgcgtgttgc
     3661 ccggcctcat cggcgtgggt ctgttcgcca ccccggggcg ggacctgcct tcgacaggcg
     3721 atggactgcg gacgctgcag tggacccgag ccgaggccga gtgctatctc tgcaccgagg
     3781 ctgccctggt tgcctgggcc cgcggcgcgc agcgcgacct gttcggtgag caccgagccg
     3841 aggccatggg ggaggccatc cgagagacgc tgagtgtact gcgggccctg gggaagtccg
     3901 atccgttcgg cggccgtctt cgggtctcgg aggagtttct cccgccggtg ttcgacgcct
     3961 accggaggct gactggtggc gagggccgga cgctcagcaa ggcgacctac ccggaactcg
     4021 ttgagttcct gccccagggg gccatcgatc ctgaggtcgt cgagaagctg gacgccatcg
     4081 cccaagtggc cgacggtgcc cgcccc
//
LOCUS       DAOSPL010000204         4106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21859_length_4106_cov_2.754628, whole genome shotgun sequence.
ACCESSION   DAOSPL010000204 DAOSPL010000000
VERSION     DAOSPL010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21859_length_4106_cov_2.754628"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1723
                     /locus_tag="PLQ54_05135"
     CDS             <1..1723
                     /locus_tag="PLQ54_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOS92674.1"
                     /translation="PAVLLANQIFNLRHLEDDGVYWEAESKRGPMLTGAYLVSSKCID
                     VLQLAYDAKSLGGYAETRFQASIWAVRTPAPDIVSAAVRLEWLCRSQSPSLKARREEI
                     ITNLTSWQGADGGWGYRPEEASNPFTTAVVLRSLARAGTPLSSPVAQKAATYLLSNQQ
                     PDGSWRCGSPILSYSATAWAVRAFADLVEAPAELRTELASLYAAAKAGQVTGPEPRAP
                     AVAATPTGAEAPTAPASGVTSALLPNPPVLQWGVSGETTLAGSFDAALRYLGGTEDYV
                     QIMGASGGAFAIRWPVEGVQIAAGTLQGEAHVRSLLAGLGRSGTYAGPADAAARWTTL
                     KTAIDAGRPCVFLKTNGEHGLLMGYDEKAMEVVAVLYGDGADAEVQRLKADDLEYVVD
                     PGPHPQAQPDPIDTLREVITNAARDASQPAADGLAHGPAAYDAWARYLTSLPAGTPPP
                     EDLVPAVKWCLDVLIDSRTQASAFLRRAAKDVPSGSAAFLIRAANEYDRLVQLLNGRE
                     FYLYSVETGKKSAAETLATPEGRKAFAELLIQCPDADAAAMDELKIALRGLGRAATAS
                     AAAPPSG"
     gene            complement(1715..1912)
                     /locus_tag="PLQ54_05140"
     CDS             complement(1715..1912)
                     /locus_tag="PLQ54_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92675.1"
                     /translation="MSISTGFFRELYRVLRPGGRAVLLTLEKRMTERLISKHHGLRVA
                     GVRVVELSGLHPSVYELEKLA"
     gene            complement(1884..2759)
                     /locus_tag="PLQ54_05145"
     CDS             complement(1884..2759)
                     /locus_tag="PLQ54_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOS92676.1"
                     /translation="MPVYYALCCPGLEDVVSAEIGECDLEVLVAEARLGWVIFAGGGT
                     GAALPVLRSVDAVGLWLGSLDGLEPGAEGLCQIEEWAASLEVSDALAHVRAIRDLPRL
                     PHFRVSAHRVGTHSYTSMDLAAAVGGGIQRDTGWPVRMRGYDINIVLRVTGRRAMAGL
                     ELCDSAAEGAGPRPGGAARLKRGVAHCLVRMLGLAGPELVMDPMCGTGSIVLECVHLH
                     PGARVLGGDMDGRALGIAAERSRAVSPAPGWVRWDARAIPLRSGCVDGVVCNLPFGRR
                     VGSHRRNEHLYGVLP"
     gene            complement(2764..3906)
                     /locus_tag="PLQ54_05150"
     CDS             complement(2764..3906)
                     /locus_tag="PLQ54_05150"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOS92677.1"
                     /translation="MSDRSRCRRWIVQPWGLCLLLYVAALVSLGVSFGAALLAIRFAP
                     ALGFIDQPSARKIHARAMPLLGGPAIAAGFASGLAWAWIGAGGEPHAGWPPHGLLLGL
                     GAFALAVGLFDDRRPLETRGKLFTLLIPSAAAGAHLWWSGRDAGIGVADACLAAAFLL
                     GLTNAMNFQDNMDGLLGGVAFAACAGIAVTLGIGSPAVGGEASRLVPPVVLAAACAGF
                     LVLNFRPARLFMGDAGSLFVGFVAGLMAVGCAFHGPPAGGTPAASGASPLIFLCLLFG
                     LPICDGALVTASRLANRRVLTAGARDHASHRLVLAGLEDRSAVLALYGVSAAFAALAV
                     LYAATRSVAVPIVAVAIVAPLWVWLYRLPIYTEREPVRPRVRQQEL"
BASE COUNT          745 a         1574 c         1295 g          492 t
ORIGIN      
        1 ccccgccgtg ctcctcgcca accagatctt caacctgcgc cacctcgagg acgatggcgt
       61 ctactgggag gccgaatcga agcgcggccc catgctcacc ggcgcctacc tcgtctcctc
      121 aaagtgcatc gatgtcctgc agctggccta tgacgccaag tcactgggcg gatacgccga
      181 gacccggttc caggcgtcca tctgggccgt tcgcacaccg gcgcccgaca ttgtcagcgc
      241 cgcagtgcgg ctcgagtggc tctgccgctc ccagtcaccg tcgctcaaag cccgccggga
      301 agagatcatc accaacctga cctcatggca aggtgccgat ggcgggtggg gataccggcc
      361 cgaggaggca tcgaacccct tcaccaccgc cgtggtgctg cgctccctgg cccgagcggg
      421 cacgccgctg agctcccccg tcgcccagaa ggcggccacc tacctcctgt ccaaccagca
      481 gcccgatggc tcttggcgat gcgggagccc catcctgtct tactccgcca ccgcctgggc
      541 ggtgcgcgcc ttcgcggatc tggtcgaagc gcccgcggag ctccgcacgg agttggccag
      601 cctctacgct gccgccaaag cgggccaggt gaccggtcca gagccccgcg cgccggccgt
      661 cgccgccacg ccaacagggg ccgaagcccc aacggcgccg gcgtccggtg tcaccagcgc
      721 ccttctgccc aacccgccgg tgctgcagtg gggcgtcagc ggcgagacca ccctcgccgg
      781 cagtttcgac gccgccctcc ggtacctggg tggaaccgag gactacgtgc agatcatggg
      841 cgcctccgga ggcgccttcg ccatccggtg gcccgtggag ggcgtgcaga tcgccgccgg
      901 gactctccag ggcgaggcgc acgttcgcag cctgctggcc ggcctgggcc gctcgggtac
      961 ctacgcgggc cccgccgatg ccgccgcccg gtggaccacg ctgaagaccg cgatcgacgc
     1021 gggccgcccc tgcgtcttcc tcaagaccaa cggcgaacac ggcctcctga tggggtacga
     1081 tgagaaagcc atggaggtcg tcgccgtgct gtacggcgac ggcgccgatg ccgaggtcca
     1141 gcggctgaaa gccgacgacc tcgagtacgt ggtcgacccc ggccctcacc cccaggccca
     1201 gccagacccc atcgacacgc tccgcgaggt catcaccaat gcggcacgcg atgccagcca
     1261 gcccgccgcc gatggcttgg cccatggccc ggcggcctat gatgcctggg ccaggtacct
     1321 gacctcgttg cccgccggaa ccccgcctcc cgaagacctg gtgcccgcgg tgaagtggtg
     1381 cctcgacgtc ctgatcgact cccggaccca ggcctcggcc ttcctccgcc gagcggccaa
     1441 ggacgtgccg tcgggctcgg ccgccttcct catccgcgcc gccaacgagt acgaccggct
     1501 ggtccaactg ctcaatggcc gcgagttcta cctctactcg gttgagaccg gcaagaagtc
     1561 cgccgcggag acactcgcca cccccgaggg ccgcaaggca tttgccgagc tgcttatcca
     1621 gtgccctgat gccgacgccg cggccatgga cgagctcaag atcgcgctgc gcgggctcgg
     1681 ccgagccgca accgcctcag cggccgcccc accgtcaggc tagcttctcg agttcataca
     1741 ccgacggatg caaaccggac agctcgacca cacggacacc ggccacccgt aacccgtggt
     1801 gcttgctgat cagacgctcc gtcatccgct tctcgagcgt cagcagtacg gcccgccctc
     1861 cggggcgaag cacccggtac agctcacgga agaaccccgt agagatgctc attcctccga
     1921 tgggagccca cgcgccgccc aaacggtagg ttacagacca caccatcgac gcagccggat
     1981 cgcaatggaa tggcgcgggc gtcccaacgc acccagcccg gcgccggcga gaccgcccgc
     2041 gagcgctccg cagcgatgcc gagagcccgg ccgtccatgt cccccccaag aacccgcgcc
     2101 cccgggtgca gatggacgca ctcgaggacg atcgagccgg tcccgcacat gggatccatc
     2161 acgagctccg gccccgccag acccagcatc cgcaccaggc agtgagccac gccccgcttc
     2221 agtcgggccg ccccgcccgg gcgcggcccg gccccctcgg cagcactgtc gcacaactcg
     2281 aggcccgcca tggcccgtcg cccggtcact cggagcacga tgttaatgtc gtagcccctc
     2341 atgcggacgg gccagcccgt gtcccgctgg atcccccccc cgacggccgc ggccaggtcc
     2401 atcgacgtgt aggagtgcgt cccgacacga tgggcgctga ctcgaaagtg cggaagccgc
     2461 ggcagatccc ggatggcgcg gacatgcgcc agggcatcgc tgacctcgag tgaagccgcc
     2521 cactcctcga tctggcacag cccctcggcg ccaggttcaa gaccgtcgag gctgcccagc
     2581 catagaccca cggcatcgac ggaacgaagc accggtagcg cggcgccggt gccaccgcca
     2641 gcgaatatga cccatcccaa ccgggcctcg gccacgagca cctccaggtc gcactcgccg
     2701 atctcagcag agaccacatc ctcgaggccg gggcagcaca gggcgtagta gacgggcacg
     2761 gggctagagc tcctgctgac gaacacgcgg ccgaacgggc tcccgctcgg tgtagatggg
     2821 cagacggtac agccagaccc acagcggagc gacgatcgca acggcaacga tgggcacggc
     2881 caccgaccgc gtcgccgcgt agagcacggc aagagcggcg aacgcggccg acaccccata
     2941 gagcgccagc acggccgagc ggtcctcgag tccggcgagc accagccggt gcgaggcgtg
     3001 gtccctggcc cccgccgtga gcactcgccg gttcgccaga cgcgacgccg tcacaagcgc
     3061 tccatcacag atcgggaggc cgaacagcaa gcagaggaag atcagcggcg atgcgccgga
     3121 ggcggccggt gtcccccccg ccggtggccc gtggaaggcg caccccacgg ccatgaggcc
     3181 agccacgaac ccgacgaaca ggctgccggc atcgcccatg aaaagccgcg caggcctgaa
     3241 gttcagaacc aggaacccag cgcacgccgc tgccagcacc acgggcggca cgagacgaga
     3301 cgcctcgcct cccaccgctg gcgacccgat ccccagcgtc acggcgatgc ccgcacacgc
     3361 ggcgaaggcc acgccaccga gaaggccatc catgttgtcc tggaagttca tcgcgttcgt
     3421 gagacccagc agaaacgcgg cggcaagaca tgcgtcggcg accccaatgc ccgcatcccg
     3481 gccagaccac cagagatgag cgcccgccgc ggccgatgga atgagcagcg tgaacagctt
     3541 gccgcgcgtc tcgagtggac ggcggtcatc gaataggcct acggcgagcg cgaacgcgcc
     3601 caggccgaga agaagcccat ggggcggcca accggcgtgc ggctcgcctc ccgcaccgat
     3661 ccaggcccac gccaggcccg acgcgaatcc ggccgcgatg gcaggtccac cgagtaatgg
     3721 catggcccgg gcgtggatct tccgcgccga gggctggtcg atgaagccca gcgcgggagc
     3781 gaaccggatc gcaagaagcg cggcgccgaa gctcacgccg agactgacta gcgccgccac
     3841 atacaacaac aagcagagcc cccatggttg gacgatccag cgccggcagc ggctacggtc
     3901 agacacgcgc accccacaaa ggttgtgtcg gccaccaacc cgcggagggg gaagtatacc
     3961 agccaccaaa gccagcggtc gcccccgggc gaagtcggaa cccggaaccc cgggccggcg
     4021 cgaaccagca ggcccccaac ccctcccgcc cgccagctac acgatccaga gcagcgcttc
     4081 gtccggatcg tcgaaacggc cgactc
//
LOCUS       DAOSPL010000205         4105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21866_length_4105_cov_3.555309, whole genome shotgun sequence.
ACCESSION   DAOSPL010000205 DAOSPL010000000
VERSION     DAOSPL010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21866_length_4105_cov_3.555309"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>927)
                     /locus_tag="PLQ54_05155"
     CDS             complement(<1..>927)
                     /locus_tag="PLQ54_05155"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOS92678.1"
                     /translation="ENVFLGREPRTRLGFVDYAAMREHSEALLAELGTPCDVTRPAEA
                     YSIATQQMVEVAKALGREADLIVMDEPTSALAEAEAERLFEVIRRLRGRGVSVVYISH
                     KMEEIYAISDRVTVLRDGEWIGTAPVGELPQERLIEWMVGRKIEQLFPKHAAEPGAEL
                     LRVDGLCVDTADGSGRRAVDGASLTLRAGEIVGLAGLRGSGCSELLGALFGRYGRVAA
                     GSVAIRGRPVSITRPAEAIANGIALLTNDRKTTGLVLSMSVLHNATLASLSRAGPPGC
                     VLRDRELALAQPFMDMLSVRTASVDAEVSALS"
     assembly_gap    928..937
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<938..1331)
                     /locus_tag="PLQ54_05160"
     CDS             complement(<938..1331)
                     /locus_tag="PLQ54_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS92679.1"
                     /translation="MAEEPLLRMEGICKSFHGVHALRGVRLDVRRGEVHSLVGENGAG
                     KSTLMRILGGAVTLDEGTITLRGRGQVFRAPKDAEAAGVAMIHQELSLVPPMSVAENV
                     FSERLPQHGGLVDYRSLYQAAVLGQALEE"
     gene            1908..3644
                     /locus_tag="PLQ54_05165"
     CDS             1908..3644
                     /locus_tag="PLQ54_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92680.1"
                     /translation="MIRIGRRLPLVSTIALAASCLGTALPAHAGASAVIGHVYNNVFT
                     PLSGDASGALSVLVSFIPPEGRHALTMGCWEMRVIDKWGTHTTTGSPIPGDTVIVPGG
                     MGRVYPWLSQSGPNGSVTIELDGSATTGGKGGSSVTPFTVSCSTTAKNIIVSDVTNDS
                     CLFPWGLMAFTPASPTQTLTVEFQDSPDPRVQVTDKGDVHLRLLHLGPNGLGSLAWSA
                     DYTNIDLGQTLELSLPVTDGTTGPVVYAPQACAQQVDQDAHQWYSTRVHVRGLSTERV
                     EVTGTRTMSLNVTHTNVLSDAAPPSGVWVSVFSSSPLGSIVDGDLSTAAGEQTDLLTD
                     VGPLTASGTYWLAVGGSDGHGSVSKYAGNVEHKIAPAMVSFTIPSATCIAGDDYGGGP
                     YAFARARAQSQYESLGCIAPPSGSPSLGCLYSARYEYGQGWRGGYQDRPSKAHTLEVL
                     FDDSVVTFNGHGGPMTIQNRENDYVSVADVEAYATAEGDSAARFVLLQCCGAGQLLLP
                     ALRAAGVDCVVGHKMSTYPTDPGWPRLMAKMCMEAKTAQVAFWETYWATPEWQMSGAT
                     IDGDTQLWGVPL"
     gene            3785..>4105
                     /locus_tag="PLQ54_05170"
     CDS             3785..>4105
                     /locus_tag="PLQ54_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92681.1"
                     /translation="MTASDRSERGYWVTVDDRWRVGLASPSGAAVLVVDCGFSKSPHA
                     AHKLASQPTPDEIEQAGREVVARVFPDFDMSEFRRSVAEVRPRSARVEVCWRQVIREN
                     GFLGG"
BASE COUNT          645 a         1389 c         1381 g          680 t
ORIGIN      
        1 cactgagcgc actcacctcg gcatcgaccg aggccgtcct caccgagagc atgtccatga
       61 acggttgggc tagcgccaac tcgcggtcgc gcagcacgca acccggcggc cccgcacggc
      121 tcagcgatgc cagtgtcgca ttgtggagca ccgacatcga cagcaccagg ccggtggtct
      181 tgcggtcgtt cgtgagcagg gcgatgccgt tcgcgatggc ctccgcgggc cgggtgatcg
      241 acaccggacg gccccggata gcgaccgagc cggcggccac ccgtccgtac cgcccgaaca
      301 gcgccccgag cagctcagag caccccgaac cccttaggcc cgccaggccc acgatctcgc
      361 cggccctgag tgtcagactg gccccatcca cggcgcggcg cccggagcca tccgccgtgt
      421 cgacgcagag gccgtcgacg cgcagaagct cggcgccggg ctcggcggcg tgcttcggga
      481 agagctgctc aatcttccgg cccaccatcc actcgatcag ccgctcctgc ggcagttcac
      541 ccaccggcgc ggtgccgatc cactcgccat cgcgcagcac ggtgacgcgg tccgagatgg
      601 cgtagatctc ctccatcttg tgggagatgt acaccacgct gacgccgcgg ccgcgcagcc
      661 gccggatgac ctcgaacagc cgctcggctt cggcctcggc caaggcgctc gtgggctcgt
      721 ccatcacgat gaggtcggcc tcacgcccca gggccttggc cacctcgacc atctgctggg
      781 tggcgatcga gtaggcctcg gcaggccggg tgacgtcaca gggagtgccg agttcagcca
      841 gcagtgcctc ggagtgctcg cgcatggcag cgtagtcgac gaaaccgagc cgggtccgcg
      901 gctcgcgccc gaggaagacg ttctcggnnn nnnnnnnctt cttcgagcgc ctgccccagc
      961 acggcggctt ggtagagact gcggtagtcg accaagccgc cgtgctgggg caggcgctcg
     1021 gagaagacgt tctcggccac cgacatgggg gggacgaggc tcagttcctg gtggatcatg
     1081 gccacgccgg ctgcttcggc gtccttgggg gcacggaaga cttggccgcg gccgcggagt
     1141 gtgatggtcc cctcgtcgag cgttacggcg ccgccgagga tgcgcatcag ggtcgacttg
     1201 ccggcaccgt tctcgccgac cagagagtgg acttcgccgc ggcgcacgtc cagcctcacg
     1261 ccacgcaggg cgtgaacgcc gtggaatgac ttgcagatgc cttccatgcg gagcagcggt
     1321 tcttctgcca tcacatgacc tgccgccgca tcctagcacc cgcgtcacgc gagcgtcaac
     1381 gcaggccgat gccggcgcct acgaacggtg tcatcacgat acgccaacgg gccgtctgct
     1441 tggaaggagc gcctgaatca gtccggggac tgagcacgtt gcccggacat ctcctcgacc
     1501 atcctcggcc ttgctggcaa cgggctctgt cccctcggtg ggcccgatgg cggccggagc
     1561 gcggagcgcc tgctcggcag cagcaaggcg cggcagggtc gcggggcagg aggcctgcgg
     1621 gcacgaagcg catggccctc gcgccaccgc gtgtcgaagg gggtcttcgg gagcgcggac
     1681 ttcagtccgc ccggctcgct cgacggccct cgatgggccg gcgagcgcca tggcgtggcc
     1741 ggcccgcggc gcccccactg accccgttca ccgcccaacc tttcgcgcgc gaatggcgtc
     1801 tattagtgaa ggagtacccc ccccgtggcg aatgaggtgt catggcggga ccctccgcct
     1861 ggcggggact cccgcatgcc gtgcacctgg tcctgcggag gtgttcgatg atccgcattg
     1921 gccgtcggct acctcttgtg tccaccatcg ccttggccgc ttcatgtctg ggcaccgcac
     1981 tccccgccca cgcgggggcc tccgccgtca tcgggcatgt ctacaacaac gtcttcacac
     2041 ctctctcggg tgacgcttcg ggtgctctgt ccgtcctcgt cagtttcatc ccgcctgagg
     2101 gccggcatgc cctgaccatg ggctgctggg agatgcgcgt catcgacaag tggggcacgc
     2161 ataccactac gggttccccg attccgggtg acacggtcat cgtcccgggc gggatgggcc
     2221 gggtgtatcc gtggctgtcc cagtctggcc ccaacggttc ggtgaccatc gagctcgacg
     2281 gctcggcaac gacgggcggc aaaggcggat cctccgtcac ccctttcacc gtgtcgtgct
     2341 ccacgaccgc caagaacatc attgtgagtg atgtgaccaa tgactcgtgc ttgttccctt
     2401 ggggtctgat ggccttcacc ccggcctcgc cgactcagac cctcacggtc gagttccagg
     2461 actcgccgga cccgcgggtc caggtgaccg acaagggcga tgtgcatctg cgtctgctcc
     2521 atctcggccc gaacggtctg ggcagcctcg cgtggtccgc tgactacacc aacatcgacc
     2581 tgggccagac cctggagctc tcgcttccgg tgacggatgg caccacggga ccggtcgtct
     2641 acgcgccgca ggcatgtgcg caacaggtcg accaggatgc tcaccagtgg tattcgacac
     2701 gggtccatgt tcggggcttg tccacggagc gcgtggaggt caccggcaca cggactatga
     2761 gcctgaacgt gacccacacg aacgtgctct cagacgccgc tccgccgtcg ggcgtgtggg
     2821 tctcggtgtt cagcagttcg cccctcggtt cgattgtcga cggagatctg agcactgctg
     2881 ccggggagca gactgacctt ctgacggatg tcggcccgct caccgcctca ggcacctact
     2941 ggctggcggt cggaggttcg gacggtcacg gcagtgtgtc gaagtacgcg ggaaacgtcg
     3001 aacacaagat cgctccggcc atggtgtcgt tcacgatccc ctcggcgaca tgtatagcgg
     3061 gggatgacta cggtgggggt ccgtacgcct tcgcccgggc gcgggcgcag tcgcagtacg
     3121 agtctcttgg gtgtatcgcg ccgccgagcg ggtctcccag tctcggctgc ctctactccg
     3181 cccggtacga gtacggacaa gggtggcgcg ggggctacca ggaccgcccc agcaaggccc
     3241 acactctgga ggtcctcttc gacgacagtg ttgtcacgtt caacggccac ggtggcccga
     3301 tgaccatcca gaaccgcgag aacgactacg tgagcgtggc agacgtggag gcatacgcca
     3361 ccgcggaagg cgactcggcc gccaggttcg ttctcctcca atgctgtggc gccgggcagc
     3421 tccttctgcc ggcccttcgg gccgcgggcg tcgactgcgt cgtgggccac aagatgagca
     3481 cgtacccgac agatcccggc tggccccgtc tcatggcgaa gatgtgtatg gaagcgaaga
     3541 ccgcacaagt cgccttctgg gagacatact gggcgacgcc cgagtggcag atgtcggggg
     3601 ccaccataga cggggacacc caactttggg gggtgccgct gtgaacggat gggtagctgt
     3661 ggcgctcgcg gcgttcatcg gcctggggac gaccgcagaa ctctgcgcgg cgcagagtgg
     3721 caaggagcag gcggcgagag ccgccatcga agcagtgagg cgagcaggtg gcgcagcagt
     3781 gcgcgtgacg gcgagtgatc ggagcgagcg cggctactgg gtcaccgtag acgaccggtg
     3841 gcgcgttggc ctggcttcgc cctcgggtgc cgccgtcctg gtggtagact gtggcttctc
     3901 gaagtcgccg cacgccgccc ataagctcgc cagccagccc acgcccgacg agatcgaaca
     3961 ggcaggcagg gaagtggtag cgcgcgtctt tcccgacttc gacatgtccg agttccggcg
     4021 ttccgtggct gaggtacggc cacggtcggc acgcgtcgag gtgtgctggc gccaagtcat
     4081 ccgggagaac ggtttcctcg gtggt
//
LOCUS       DAOSPL010000206         4096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_21933_length_4096_cov_3.466469, whole genome shotgun sequence.
ACCESSION   DAOSPL010000206 DAOSPL010000000
VERSION     DAOSPL010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21933_length_4096_cov_3.466469"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1440)
                     /locus_tag="PLQ54_05175"
     CDS             complement(<1..1440)
                     /locus_tag="PLQ54_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92682.1"
                     /translation="MAAGARRREQTHIKRTKMQREIPLFVSGKPCACIVTPEGDDDLY
                     DQGGLISAFLEDIHGIELPVLTCDEHAGGYRTRKFNVIALGDFSSNRVLRDLYVAGRH
                     YVDRRFPGGRGHVVRTICDPGSEGRSYVVIGGSTPAGLQNAVHAFCELLVALGSRRNI
                     PPTHLCVCEHDEEYALGAAELSERIDRGLEALSVGDSASALDAAADAAVRYDVSGVPT
                     WARLFASILEAYAEASAEAGTPAVDASFDAARWLWRLVPIWDNIAEFGAFSDAERDLL
                     GLTLHSWSEWLFARMRAEAPSRSGSGPGTWRDVAAGLGLVAAGRYFRTYYHVMAGREW
                     VELGKAWIESASRAGRLMAECALDECRAMDMTLSYALRYQGGLQADEADLLLFRERML
                     MALAAEPAPAAPSVSTELEQHVGAVLRKAVFVTQSKRLRGETLGNATVLGGFHEDEPE
                     AAPVRPRPPLAIPVSPKLRPWLRAQWPTDA"
     gene            complement(1561..2511)
                     /gene="miaA"
                     /locus_tag="PLQ54_05180"
     CDS             complement(1561..2511)
                     /gene="miaA"
                     /locus_tag="PLQ54_05180"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOS92683.1"
                     /translation="MPESPPKPTLLVIVGPTATGKSQAAMELAEQVGGEIVSADSMQA
                     YRGMDIGTAKPTPEERARVHHHLVDVVEPDEPFNVATFAELARAAIAEIAGRGALPIV
                     VGGSGLYVRSITCRLDLPLVSPDDEVRARLEAEAVSEGRQALHDRLRSVNPAAAAQIH
                     PNDLKRIVRALEVYEKSGRPISEVYREAPEPTDEYRCIVYGARRERDRLYQGIEARCD
                     RMIRQGLVQEVEGLLSRGFGRDLQSMQAIGYKEVVAHLEGLYGFAEMMAVFRRNSRQF
                     AKRQMTWFGREPNVCWVDVDEENLVERIRHDHPGLSGPGA"
     gene            complement(3177..3593)
                     /locus_tag="PLQ54_05185"
     CDS             complement(3177..3593)
                     /locus_tag="PLQ54_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92684.1"
                     /translation="MKTLRWLLPCVLVLGLVGGIVGAKVLSINASKSATQAGAASAGP
                     GAGHSCPEGYQGCAAGDPCPDCQAKDAKKSEVHQTSGTKPAIEPEECEKAHAEGSGCP
                     LETKGEDAKSGGCPFEAAAKDTKDEGGHEEGGCPAH"
     gene            complement(3721..>4096)
                     /locus_tag="PLQ54_05190"
     CDS             complement(3721..>4096)
                     /locus_tag="PLQ54_05190"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOS92685.1"
                     /translation="VAKSMSVSDGDGTITNSAGAVGEEAIFWKPAEWCDYSGAVSPGV
                     VNGIALFSHPANGNHPPDWHVRSDGWMGACLSRESECVVAVDAPLLLRYGLYVHRGGV
                     DEAGVASRFEAFATSALAPCQA"
BASE COUNT          674 a         1520 c         1270 g          632 t
ORIGIN      
        1 tgcatcggtc ggccactggg cacgcagcca gggtcggagc ttcggcgaca cgggaatcgc
       61 cagcggagga cggggcctca ccggggccgc ttcaggctcg tcctcatgga acccgcccaa
      121 caccgtggcg ttccccaacg tctcgccccg caaccgcttg ctctgggtca cgaacaccgc
      181 cttgcgaagg acggcgccca cgtgctgctc cagctcggtg gacaccgagg gggcggcggg
      241 ggctggctcg gcagccaggg ccatcagcat gcgctctcgg aacaggagca gatccgcctc
      301 gtcagcctga agcccgccct ggtagcgcag ggcataggag agggtcatgt ccatggcccg
      361 gcactcgtct agtgcgcact cagccatgag gcggccggca cgggacgcgg actcgatcca
      421 ggctttgccc agctcaaccc actcacgtcc ggccatcacg tggtagtagg tccggaagta
      481 ccggcctgct gccacgaggc cgaggccggc cgccacatca cgccatgtgc cgggcccaga
      541 tcccgagcgc gacggtgcct cggcccgcat tcgcgcgaag agccattccg accacgagtg
      601 gagcgtcagg cccaggaggt cgcgctccgc gtcgctaaac gcgccgaact cggcgatgtt
      661 gtcccatatc ggaaccaggc gccacaacca gcgcgcggcg tcaaaggagg catccacggc
      721 cggggtgccg gcctcagccg acgcctcggc ataggcctcg agaatcgacg cgaacagacg
      781 cgcccatgtg ggcacgcccg agacgtcata gcgcacggcc gcatcggcag cggcatccag
      841 agccgacgcc gagtcgccaa cgctcagggc ttcgagccct cggtcgatcc gttcggagag
      901 ctcggccgcc cctagagcgt actcctcatc gtgttcgcac acgcacaggt gggtcggggg
      961 aatgttgcgc cgcgagccca atgccacgag gagctcgcaa aaagcgtgca ccgcgttctg
     1021 aagacccgcc ggcgtgctgc cgccgatgac cacatacgaa cggccctcgg atcccgggtc
     1081 acagatggtg cgaaccacgt gcccccggcc gcccggaaag cggcggtcga cgtagtgccg
     1141 cccagccacg tacaagtcgc gaagaacccg gttggacgag aagtcgccca gggcgatcac
     1201 gttgaacttg cgcgtgcggt aaccacccgc atgctcatcg caggtgagca ccggcagttc
     1261 aatcccgtgg atgtcctcga gaaacgcgga gatcagaccg ccttggtcat agaggtcgtc
     1321 gtcgccctcc ggcgtcacga tgcatgcgca gggcttgccg ctgacgaaca gcgggatctc
     1381 ccgctgcatc ttggttcgct tgatatgggt ctgctctctt ctccgcgcgc ccgccgccat
     1441 gcgccctcgc gtatcccgat tgaatcggcc gcccgtcccg gcctccgccg ccccccgcaa
     1501 cccaacagcc ggttgcagcg tccaggttcg ccccacaacg cgtcgaacct tcggaacccc
     1561 tcaagccccc ggcccactca ggccaggatg atcgtgccgg atccgctcca ccagattctc
     1621 ctcgtccaca tcaacccagc acacattggg ctcccggcca aaccaggtca tctgccgctt
     1681 cgcgaactgc cgcgagttgc gccggaacac cgccatcatc tcggcgaatc cgtacagacc
     1741 ctccaggtgc gcgacgacct ccttgtaccc gatcgcctgc atcgactgca ggtcgcgccc
     1801 gaacccgcgc gacagcagtc cctccacctc ctggaccaag ccctgccgga tcatgcggtc
     1861 gcaccgcgcc tcgatgccct gatagagccg gtcacgctcg cgacgggcac cgtagacgat
     1921 gcaccggtat tcgtcggtgg gttcgggggc ctctcggtag acctccgaga tgggcctccc
     1981 ggacttctcg tagacctcga gggcccgcac gatccgcttc aggtcgttgg ggtgaatctg
     2041 ggccgcggcc gccgggttga ccgagcgcag ccggtcatgc aacgcctgcc gcccctcgga
     2101 caccgcctcg gcctcaagcc gcgcgcgcac ctcatcgtcg ggcgatacaa gtggcagatc
     2161 gagccggcag gtgatcgacc ggacatagag gccggagcca cccaccacga tgggaagcgc
     2221 cccccgtcct gctatctccg cgatggcggc ccgggcgagc tccgcgaaag tggcgacgtt
     2281 gaacggctca tcgggctcaa caacgtcaac caggtggtga tgcactcgag cccgttcctc
     2341 gggcgtcggc ttcgcggtgc cgatgtccat cccccggtac gcctgcatcg agtcggccga
     2401 gacgatctcg ccgccgacct gctcggccag ctccatcgca gcctggctct tgccggtggc
     2461 cgtggggccg acgatgacga gcagtgtggg cttcgggggc gactcaggca tggcagacgt
     2521 gattctaggc ggtgcagaga ccgctgtcga cgggggctcg gccgcgccgc ccctcaggtt
     2581 cccgtggcca cgtgagcgta catcgattgc agcgcgacac ggcccggccc ggtgaagcgg
     2641 ttccagacga tccgtcccgc gctcgcgagc gtcgacggac tgtcccgccg ccgggtggac
     2701 ggggaacacg tgccccacgc catctctgct gaacgcgatc cgcccttgtc gcgtcgcctg
     2761 ggtcagggag atcggcagtc ccgcgaggag gcgtcggacc gctcccgcca acggccggat
     2821 gccgacctcg accgatgggt ccttccagag cagaacgtgg tgctcgaagt agatgagcat
     2881 cgaacgcagc tcgaccccaa gcgcgggcac gagctcgccc tcgatgtggt aggccccccc
     2941 gcgaccgtcg ctccgcggac tgcagtcggc atctggatgg gtaccgcccg cggcatccgg
     3001 cccctcggtg agctgctggg catacctcgg gctccacagt accccgacgc cgggcccacg
     3061 gcgcggttgc gccacgaaca tgcaagagcc cgcgcagcca cccggtaggg tatggcacgc
     3121 gggctctcac cctgtcagca ctaaggctac gctcccggct cagctttgtc ctcggcctag
     3181 tgtgcggggc agccgccttc ctcgtgccca ccctcgtcct tggtgtcctt ggcggcggcc
     3241 tcgaatgggc atccgccaga cttggcgtcc tcgcccttgg tctccagcgg gcacccgctg
     3301 ccctcggcgt gggccttctc gcactcctca ggctcgatcg cgggcttggt cccggatgtc
     3361 tggtgcacct cgctcttctt ggcgtccttg gcctggcagt cggggcacgg gtcgccggcc
     3421 gcgcacccct ggtacccctc cgggcacgag tgcccggccc cggggcctgc actggccgct
     3481 ccggcctgcg tcgcactctt tgatgcatta atgctcagta ctttcgcccc gacgattccg
     3541 cccacgaggc cgagaactag gacacatggc aagagccacc gcaaagtctt cactcgatcg
     3601 agccctccat tcgcaattgg ccgggccatg ctccccgtcg ggacggcacg gctcaggcat
     3661 acccaccatc aggacgcgca gcctcgtcgt tccacgcagc ccagcccgtc gtgcccaccg
     3721 ctaggcttgg cacggagcca acgccgacgt ggcgaatgcc tcgaacctcg acgccacacc
     3781 ggcttcgtca acgccgccgc ggtgcacata gagaccatac cgcagaagca ggggggcgtc
     3841 taccgccacc acgcactccg actccctcga taggcaggcc cccatccatc catctgagcg
     3901 aacgtgccag tcgggggggt ggttcccgtt cgccggatga ctgaacaggg caatgccatt
     3961 cacgaccccc ggcgataccg cacccgagta gtcgcaccac tccgccggct tccagaagat
     4021 ggcctcctca cccaccgccc cggccgaatt cgtgatcgtc ccatcgccat cactcaccga
     4081 catgctcttg gcgacc
//
LOCUS       DAOSPL010000207         4077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22057_length_4077_cov_2.903033, whole genome shotgun sequence.
ACCESSION   DAOSPL010000207 DAOSPL010000000
VERSION     DAOSPL010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22057_length_4077_cov_2.903033"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1411
                     /locus_tag="PLQ54_05195"
     CDS             <1..1411
                     /locus_tag="PLQ54_05195"
                     /inference="COORDINATES: protein motif:HMM:NF019229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HOS92686.1"
                     /translation="PSISAAELFVDATEASGIGLHSQALGVTAADFDGDGDVDLVFAD
                     TSDAEMLLYRNEGGLVFEPEVVAGPQTNVEFAAPIDYDGDGDLDLICGGWPRKWSLLA
                     NGGDGTFEDATATRGLATQEAVETAGIAVGDVDVDGDLDVYLGRGRLPDVLYLNDAGR
                     SFVSAEGTLWASGLLPEATGTEHALFADLTGDARPDLWVSRYGHECTYYVNDPHGTLV
                     DESERRGLWDLGPDFGSAVIDADGDGDTDLYLIRGGWHRDTNRLLLNDGKGAFLPGTE
                     ASGLGDEGTGFGCAVGDVDLDGDEDLYITNDGSGNRLFLSDGRGRFVDVTPEALRLAG
                     TSTRGAVLADLDGDGDLDLVVRCRRAHDRVFRNTTDSPNWLKVRVDGPGLNRFGIGAR
                     VRVFDGGSLGDLAHLRGQREIVAACGWCSAPPPEAHFGLPADGRYDVEVVLPGGTTWT
                     ATDVPATQTLVVSSPVAGP"
     gene            1455..2645
                     /locus_tag="PLQ54_05200"
     CDS             1455..2645
                     /locus_tag="PLQ54_05200"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:NF003242.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOS92687.1"
                     /translation="MKYVLVIPDGSADLPCPELGGRTPLEAAHIPNMDRIAREGATGL
                     VQTIPEGMRPGSEVAILGILGYDPITCFPGRGPLEALAMGIELSPTDAVYRCNLVTTD
                     GERLVDFTAGHITSEEAAELMTSLNGQPGLEDLRFYPGVAYRHIMVWAGGPTGAETRA
                     PHDHVGDLLDGVLPRGEGSGRLREAFWRAHELLSSHGVNRRRRNEGHNPGNGIWPWGQ
                     GRVKSAPSFRERFGVGGSVIGAVDLVKGIAEYGGLAHPNVPGATGFADTDLKAKLAHA
                     LDALAVEDFVAVHVEAPDEETHAGRLEGKIGTIERIDAEIVGPLLEALPSLGPHRVLV
                     LPDHFTLLSTRTHAADPVPFAIWPAPESIGPHPATDFSEPAARTTGLALSSADQLVPL
                     LVGK"
     gene            complement(2688..3029)
                     /locus_tag="PLQ54_05205"
     CDS             complement(2688..3029)
                     /locus_tag="PLQ54_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOS92688.1"
                     /translation="MRDEAYALDMVLAATEARDLCAGSTLEAFLADRVLQLAVTHVVQ
                     AIAEAASRTSEAFRAEHPSVPWRAIVGMKHRLVHDYSGTDFSLVWDVVQNHLPPLLAA
                     LSGMFPPDNEA"
     gene            complement(3022..3333)
                     /locus_tag="PLQ54_05210"
     CDS             complement(3022..3333)
                     /locus_tag="PLQ54_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOS92689.1"
                     /translation="MAHGLPVTQEGLRQFCDRHGVRRLSLFGSAVRGELGPESDVDVV
                     VEFRDGVAVTLMLLLDMEEELSKLFGRPVDLLRREAIEASPNYIRRRGILDSLEPLVD
                     A"
     gene            complement(3414..>4077)
                     /locus_tag="PLQ54_05215"
     CDS             complement(3414..>4077)
                     /locus_tag="PLQ54_05215"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS92690.1"
                     /translation="LGSPAARRVAPGAKPLGIGWPALGVLVLAGVLFSLRDFASWGIW
                     ILTPLYLQQAFEFSVARSGFYLGLNCLPGIVSTPLLGARSDGPRRWRWLLGTLVGAAA
                     AQSLVPRVPGSLCFVPLIVAGFFSTASFTISDAALAEIFPPRVRGRVFGLFITLCLGL
                     SSSAQWAVGHWVDRSSLVVTAQSAYHAPFAVLGLVFLSSASALPALRWLRRVVEKGSA
                     VG"
BASE COUNT          678 a         1373 c         1422 g          604 t
ORIGIN      
        1 tccgagcatc tcggccgccg agctgttcgt cgatgccacg gaggcgtcgg gcatcgggct
       61 gcactcgcag gctctgggtg tgaccgccgc ggacttcgat ggtgacgggg acgtcgacct
      121 ggtgttcgcc gacaccagcg atgcggagat gctcctctat cgcaatgagg gtggcctggt
      181 cttcgagccc gaggtcgttg ccggtccgca gaccaacgtc gagttcgcgg cacctatcga
      241 ctatgacggc gatggcgacc tcgacctgat ctgtggcggc tggccgcgga agtggagcct
      301 gctggccaat gggggcgatg gcaccttcga ggacgcgacc gcgacccggg gtttggccac
      361 ccaggaggcc gtggaaactg ccggaatcgc ggtgggcgat gtggatgtgg acggtgacct
      421 agacgtatac ctcggccgtg ggaggctgcc ggatgtgctc tacctcaacg acgcgggacg
      481 gagcttcgtc agcgcggaag gcaccctctg ggcgtccggg ctccttcccg aggccaccgg
      541 caccgagcac gcgctgttcg ctgacctcac cggcgatgcg cggcctgacc tgtgggtctc
      601 gcggtatggc cacgagtgca cctactacgt caacgacccg cacggcacgc tggtcgatga
      661 gtccgagcgc cgcgggctgt gggacttggg gccggacttc ggctctgccg tgatcgatgc
      721 ggatggcgac ggcgacacgg atctgtacct gatccgcggg gggtggcatc gcgacaccaa
      781 ccggctgtta ctgaacgacg gcaagggcgc gttccttccc ggcacggagg cgtcgggcct
      841 gggtgacgaa gggaccggct tcggttgcgc cgtgggcgat gtggatctcg acggagacga
      901 agacctctac atcaccaacg atggcagcgg taaccggctg ttcctgagcg atggacgcgg
      961 ccgcttcgtc gacgtcacac cggaggcgct gcgtctcgct ggcacctcga cgcggggtgc
     1021 ggtgctcgct gacctcgatg gcgacggcga cctcgacctt gtcgtgcgct gccggcgagc
     1081 gcacgaccgg gtcttccgca acacgaccga tagccccaac tggctgaagg tgcgggtcga
     1141 tggccccggg ctgaaccggt tcggcattgg cgctcgcgtg cgcgtgttcg acgggggatc
     1201 gctgggcgac ctggcgcacc tgcgtgggca gcgggagatc gtggccgctt gcggctggtg
     1261 ctcggctccg ccgccggaag cgcacttcgg cctcccggcc gacggccggt acgacgtgga
     1321 ggtcgtgctt cccggcggca ccacgtggac ggcgacggac gtgcccgcaa cccagacgtt
     1381 ggttgtgagt tcgccggtcg cggggccata atgggcgctg cagagcacac ctggcggaat
     1441 gggtcgtgtc gccgatgaag tacgtccttg tgatccccga tggctccgcc gacctcccat
     1501 gccccgagct gggcggtcgc actccgcttg aggccgcgca catccccaac atggaccgca
     1561 tcgcgcgcga gggtgcgacg ggcttggtac agaccattcc ggaaggcatg aggccgggca
     1621 gcgaggtggc gatcctcggt atcctggggt acgatccgat cacatgcttc cccggccgcg
     1681 gcccgctcga ggcactcgcc atggggatcg agctctcgcc caccgacgcc gtctatcgat
     1741 gcaacctggt caccaccgat ggcgagcgac tggtggactt cacagccggg cacatcacct
     1801 cggaggaagc cgccgagctg atgacatcgc tcaacgggca gccagggctc gaggacctgc
     1861 gcttctaccc cggcgtggcc taccggcaca tcatggtctg ggccggtggc ccgaccgggg
     1921 ccgagacccg ggcgccgcac gaccatgtgg gtgacttgct ggacggcgtc ctgccccggg
     1981 gcgagggatc agggcgtctg cgcgaggcct tctggcgcgc ccatgaactg ctcagttcgc
     2041 acggagtgaa ccgtcggcgc cggaacgagg gccacaaccc cggcaacgga atctggccgt
     2101 ggggccaggg ccgggtcaag tcggcgccgt cattccggga gcgctttggc gtgggcggca
     2161 gcgtcattgg ggccgtcgac ctggtgaagg gcatcgccga gtatggtggg ctggcccatc
     2221 cgaacgtccc cggcgccacg gggttcgcgg acaccgacct gaaggcgaag ctggctcacg
     2281 cactggacgc tctggccgtc gaggacttcg tcgcggtcca tgtcgaggcg ccggacgagg
     2341 agacccacgc cggccggctc gagggcaaga tcggcaccat cgagcgcatc gatgccgaga
     2401 tcgtgggccc gctgctcgag gcactgccct ccctgggacc acaccgggtg ctggtcctgc
     2461 cggaccactt cacactgctg tccacccgca cccatgcggc cgacccggtg cccttcgcca
     2521 tctggccggc gccggagtcc atcggcccgc acccggcgac ggacttctcc gagcccgccg
     2581 cacgcaccac ggggctcgca ctctccagcg ccgaccaact cgttccgttg ctggtgggca
     2641 agtagggccg cgccgctctc gcaccggcga atcgcgacgg cgcgtggcta tgcctcgttg
     2701 tccggcggga acatgccgct gagtgcggcc aggaggggtg ggaggtggtt ctgcaccacg
     2761 tcccagacga gggagaagtc cgtgccagag tagtcatgaa ctagccggtg cttcatgccc
     2821 acaatggccc tccacggaac cgatgggtgc tctgcgcgga acgcctccga cgttcggctg
     2881 gctgcctcgg cgatggcctg gacaacatgc gttaccgcga gctggagcac ccgatcggcc
     2941 agaaatgcct cgagtgtgct gccggcgcat aggtcccggg cttcagtggc ggcaagcacc
     3001 atgtcgagcg cataggcctc atcacgcatc aacgagaggc tccaggctgt caagaatgcc
     3061 gcgcctgcga atgtagttcg ggctggcctc aatggcctct cgcctcaaca ggtcaacggg
     3121 ccgcccgaag agcttcgaga gctcctcctc catgtccagc aggagcatga gcgtcacggc
     3181 gactccgtcg cggaactcga caacgacgtc gacatcgctc tcgggaccca actcgccgcg
     3241 gaccgcggag ccaaacagcg atagccgccg gacgccatgg cggtcgcaga actgtcgcag
     3301 gccctcctga gtcactggca gtccgtgggc cactgcagca cctcccctcg atgcgagccc
     3361 agtgtacact tccctgcgca gccacgggag acccatcgcc ccggccgacg cgactatccc
     3421 accgcactcc ccttctccac cactcgccgg agccagcgga gggccggcag ggcggaggcg
     3481 ctggagagga agacgagccc aaggactgcg aatggcgcgt ggtatgccga ctgcgccgtg
     3541 acgaccagcg acgagcggtc gacccagtgc cccacggccc actgggccga ggatgagagc
     3601 ccgagacaca aggtgatgaa cagcccgaag actcggccgc gcaccctcgg tgggaagatc
     3661 tccgccaggg ccgcatcgga tatcgtgaag ctggccgtcg agaagaagcc ggccacgatg
     3721 aggggcacga agcacaggct tcccggtacc cggggaacca acgactgggc agccgccgcg
     3781 cccaccagtg tgccgagcag ccatcgccag cggcgtggac cgtcggatct tgcacccagc
     3841 agcggcgttg agacgatgcc cggcaggcag ttcaggccga ggtagaagcc cgatcgtgcc
     3901 accgagaact cgaaggcctg ctgcaggtag agcggcgtga ggatccagat cccccacgag
     3961 gcgaagtcgc gcaagctgaa cagcacgccc gcgagcacga ggacgcccaa cgcgggccag
     4021 ccgatgccga gaggcttcgc gccgggcgcc acgcgccggg cggcaggaga cccaaga
//
LOCUS       DAOSPL010000208         4076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22070_length_4076_cov_2.778662, whole genome shotgun sequence.
ACCESSION   DAOSPL010000208 DAOSPL010000000
VERSION     DAOSPL010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22070_length_4076_cov_2.778662"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="PLQ54_05220"
     CDS             <1..804
                     /locus_tag="PLQ54_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92691.1"
                     /translation="TASWPVFALALVWMAVVFFITYFAISMAIGRIRAEMGMPAHDFH
                     FAGPDQMLPNILGTRRFPPRALAIDSMYWWFNRASRAHVLPHQAEAFKIAQRTGMSNS
                     SIVGALTVAMVIAMPVAFWAMLQCDYKYGVAAQMSGGAAGFGNEPMSKLASWLNAPTG
                     FSIQRLLAAIIGAVFLFTTMMLKTRVSWWPIHPVGYAVSASWSMQFLWCPLLIASGIK
                     WAVTRYGGHRSVQSLVPLAFGMILGDLTGGSFWTLYSLWKKVTVYSIWQ"
     gene            1068..2240
                     /locus_tag="PLQ54_05225"
     CDS             1068..2240
                     /locus_tag="PLQ54_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92692.1"
                     /translation="MTGRSQPTVVGLAVICLLALGLGTAFAVELTVAGAGATNVVSGT
                     VKLNVSAPGAEGGYVILKVDDKFISSQAPPFDFVWNSRTVEDGQHRITVTEVNADGKV
                     ADKASQVVRVENRATVTGPVTLAYQFRQGAKATYTITGQTDIKELGANRYYEFVPWNL
                     YSSYAGRFQVAAAETVTNTQAGQAVIERALTAGEVDLPEVISRLLGVGKTTQVNLTTA
                     GALKDGPGVVNTECKFGAAWIALPEKPVQEGDKWNGPLVAQFEVQDGRTVMVPAEHRV
                     VDFVKDHGVDCALIESTYSQRGPLSLRIYGQVRELDSVFVEGQRKTYFAIKQGCIVRM
                     EDTLKGEFTIKSADFGMYAATPAGPTIPGAPAPVGRGVPETVNLQLLMNLTSDMDQ"
     gene            2275..3264
                     /locus_tag="PLQ54_05230"
     CDS             2275..3264
                     /locus_tag="PLQ54_05230"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOS92693.1"
                     /translation="MDDPIFLKHRCHEPHRENPMRLIGLGAAVTEALGEPTRLPVRPA
                     TEAELRAGHTAEYIELVRRSSREHGWLDADTYLCPDSCEVASHAVGAAIDAARCVASG
                     AADGAVCLIRPPGHHAEPSRGMGFCIFNNVGVAARVALEAGWVRRVAVVDFDVHHGNG
                     TQAMLWEEPRALFVSIHQWGIYPLTGPAHEVGGPGAEGTHINVPLPRGAGDADYLAVI
                     ARVVVPMLSRFGPELVLVSAGYDAAAADPLGGARLTPRGFAAMAAALREVAPRPVLCL
                     EGGYDPPALKACLTAQVRVWAGRDAGPRTLEGEPSHAAASVIAVVREIHGIET"
     gene            3294..3716
                     /locus_tag="PLQ54_05235"
     CDS             3294..3716
                     /locus_tag="PLQ54_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92694.1"
                     /translation="MPGPGTAVLWVAVFGVGLVFFLLWELTQWRRVGTVLSRRQKALR
                     LVNIGFLGVILGLMFVGSMGWVRTVAGQLSLLMAILGLTFIVLALALRDWRELAATRA
                     ANEMRIAGDLARLLSAHAERQRLRAAGGDAGTPEDEAR"
     gene            3748..>4076
                     /locus_tag="PLQ54_05240"
     CDS             3748..>4076
                     /locus_tag="PLQ54_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92695.1"
                     /translation="MIVDLVVAVSSLWQWWNNPFLVRSLRRDWRGRDVLRWLYTYTLL
                     LGLIFGLVLVAARCIGPRGFHEHWGGSWGGLAGTLVAAVHTVLVWRASATGRWREWIA
                     VERQAGAL"
BASE COUNT          623 a         1309 c         1461 g          683 t
ORIGIN      
        1 accgccagct ggccggtgtt tgccctggcg ctggtgtgga tggccgtggt gttcttcatc
       61 acctactttg ccatctcgat ggccattggc cggattcggg ccgagatggg catgccggcc
      121 cacgacttcc acttcgccgg gccggaccaa atgctgccca acatcctcgg gacgcggcgc
      181 ttcccgccca gagccctggc catcgactcc atgtactggt ggttcaaccg agcctccaga
      241 gctcacgtgc tgccccatca ggccgaagca ttcaagatcg cccagcgcac cggtatgagc
      301 aactcaagca tcgtgggcgc gctgacggtc gccatggtca tcgccatgcc cgtggccttc
      361 tgggcgatgc tgcagtgcga ctacaagtac ggcgtggcgg cccagatgag cggcggcgcg
      421 gcgggctttg ggaacgaacc catgtcgaag ctcgcttcgt ggctgaacgc gccaaccggg
      481 ttcagcatcc aacgcctcct cgcggcgatc atcggagccg tgttcttgtt caccaccatg
      541 atgctgaaga cccgggtctc gtggtggccc atccatccgg tggggtatgc cgtgtcggcg
      601 agctggtcca tgcagtttct gtggtgcccg ctgctgatcg cgtcgggcat caagtgggcg
      661 gtaacccgct acggcggcca ccggtcggtc cagtcgctgg tgccgctggc tttcggcatg
      721 atcctgggcg acctgaccgg tggaagcttc tggacgctgt acagcctgtg gaagaaggtg
      781 acagtgtaca gcatctggca gtgagcctga tggccgcagc ggggcccggc gacctccgca
      841 ccatgggcgg cgcggccgtc ggtgtgctgg gatgagcccc cggccggccc gtggtctgtc
      901 cgccatggga ccttcggtga aggagacagc ggccggcggg tccgaatagg gcaaggacga
      961 gggcggtcat gagagccacc gccccatgtg gaatgcggat gcgggtcgcg gcccggtgga
     1021 cgcgaccgtt cggtgttacg gtgcgtgcgc ggagaggaga tcgggacatg accgggaggt
     1081 ctcaaccgac agtcgtgggg cttgccgtga tctgcctact ggctctgggg ctcggcacgg
     1141 cgttcgccgt ggagctgacc gtcgccggtg ccggggcgac caacgtggtc tcgggcacgg
     1201 tgaagctgaa tgtgtcggcg ccaggggctg agggcgggta cgtcattctg aaggtcgatg
     1261 acaagttcat ctcctctcag gcaccgccct tcgatttcgt gtggaacagc cgcaccgtcg
     1321 aggacggaca acaccgtatc accgtcaccg aggtgaacgc cgacggcaaa gtagcggaca
     1381 aagcgtccca ggtggtgcgc gtggagaacc gggccacggt gacgggtccg gtgaccctgg
     1441 cttaccagtt ccgccagggc gccaaggcga cgtacaccat cacgggacag accgacatca
     1501 aggaactcgg cgccaaccga tactacgagt tcgtgccgtg gaacctctac tcgtcctatg
     1561 ccgggcggtt ccaggtggcc gccgccgaga cggtcaccaa cacgcaggcc ggccaggcgg
     1621 tcatcgagcg ggcgctcacc gccggcgagg tggatctgcc cgaggtcatc tcgcgcctgc
     1681 tgggcgtggg gaagacgact caggtgaatc tgaccacggc cggcgccctc aaagacggcc
     1741 cgggtgtggt caacaccgag tgcaagttcg gcgcagcgtg gatcgctctc ccggagaagc
     1801 ccgtccagga aggcgacaag tggaacggac cgctcgtggc ccagttcgag gtccaggacg
     1861 gccgcacggt catggtaccg gcagagcacc gggtggtgga cttcgtcaag gaccacggcg
     1921 tcgactgcgc gctgatcgag agcacgtaca gccagcgcgg cccgctcagc ctgcgcatct
     1981 acggccaagt gcgtgagctc gacagcgtgt tcgtcgaggg ccagcggaag acgtacttcg
     2041 cgatcaagca gggctgcatc gtccgcatgg aggacacgct caagggggag ttcacgatca
     2101 agtcggcgga cttcggaatg tacgccgcca cgcctgccgg gccgaccatc cccggcgccc
     2161 cggctccggt cggccgtggt gtccccgaga cggtgaacct ccagctcctg atgaacctga
     2221 cctcagacat ggaccagtag ggccggcaga gggcgcatcg cattgtgggc cctcgtggat
     2281 gacccgatat tcctcaagca ccgctgccac gagccgcacc gggagaaccc gatgcggctc
     2341 attggactgg gcgcggccgt gaccgaggcg ttgggcgagc cgactcggct tccggtgcgc
     2401 ccggccaccg aggcagagct tcgggccggc cacacggccg agtacatcga gctcgtgcgt
     2461 cgctcgtcgc gcgaacacgg atggctggac gcggacacgt acctctgccc agactcctgc
     2521 gaggtggcgt cccacgccgt cggcgccgcg atcgatgcgg cccgctgcgt cgcttccggt
     2581 gcggccgatg gcgccgtgtg cctcattcgc ccgcccggac accacgccga accatcccga
     2641 ggcatgggat tctgcatctt caataacgtc ggtgtggccg cccgggtcgc cctcgaggcc
     2701 gggtgggtcc gtcgcgtggc cgtggtcgac ttcgatgtgc atcacggcaa cggcactcag
     2761 gccatgctct gggaggaacc ccgcgcgctg ttcgtgtcga tccaccagtg ggggatctac
     2821 ccgctgactg ggccggcgca cgaggtggga gggccggggg ccgagggcac ccacatcaat
     2881 gtgccgctgc cccggggggc gggagatgcc gattacctgg cggtgatcgc ccgggtcgtg
     2941 gtgcctatgc tgtcccggtt cgggccggag ctggtgctgg tgtcagccgg atatgacgcg
     3001 gcagccgcgg atccgctggg cggagctcgg ctgacgcctc gaggcttcgc cgccatggcc
     3061 gccgcgctcc gcgaggtggc gcccagaccg gtgctgtgcc tcgagggagg atacgacccg
     3121 ccggcgctga aggcgtgctt gaccgcccag gttcgagtgt gggcaggccg cgacgcgggc
     3181 ccacggacac tcgagggcga accatcgcac gccgccgcct cggtgatcgc ggtggtgcgg
     3241 gagatccacg ggatcgagac ataagtcgca gccgggaacg gaggtggacg gccgtgcctg
     3301 ggccgggaac cgcggtgctc tgggtggccg tcttcggtgt cggcctggtc ttcttcctgc
     3361 tgtgggaact gacgcagtgg cgccgcgtgg gcaccgtcct cagccggcgg cagaaggcgt
     3421 tgcgcctggt gaacatcgga ttcctcggcg tgatcctggg cctcatgttc gtcggaagca
     3481 tgggctgggt ccggaccgtg gccgggcagc tgtccctgct gatggcgatt ctggggctga
     3541 ccttcatcgt gttagccctg gccctccgcg actggcgtga gctggcggcc acgcgggccg
     3601 ccaacgagat gcgtatcgcc ggcgacctgg cgcggctgct gagtgctcac gccgagcgcc
     3661 aacgactccg ggcggcggga ggagacgcgg gcacccccga ggacgaggcc aggtgatggg
     3721 ctcgacagcc tctcggaccg cacctcgatg atcgtcgacc tggttgttgc cgtctcttca
     3781 ctctggcagt ggtggaacaa tcccttcctg gtgcgcagtc tccggcgcga ctggcgaggg
     3841 cgggacgttc tccgttggct ctacacgtac acgctcctgc tcggtctgat cttcggtctc
     3901 gttctggtgg ccgcacggtg catcggacct cgcgggttcc acgaacactg gggggggagc
     3961 tggggtggac tggcggggac actggtggcc gcggtgcaca ccgttctcgt gtggcgcgcg
     4021 tcggcgaccg gccgctggcg agagtggatc gcagtcgagc gtcaggcggg cgccct
//
LOCUS       DAOSPL010000209         4072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22108_length_4072_cov_3.450336, whole genome shotgun sequence.
ACCESSION   DAOSPL010000209 DAOSPL010000000
VERSION     DAOSPL010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22108_length_4072_cov_3.450336"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..2373)
                     /locus_tag="PLQ54_05245"
     CDS             complement(175..2373)
                     /locus_tag="PLQ54_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92696.1"
                     /translation="MRCPRFAALPRLYRPALAAALAVLPSAVMSAQPRDVLSDTWTAT
                     DALGRELPGFHECGPPRAGRQVGIFYFLWLGEHAGGQGPFDNSKILAADPNAINDKDH
                     PLWGPMHAFHHWGEPLFGYYQTDDAYVLRKHAQMLSDAGIDVIIFDVTNQITYRRWYL
                     ALLDAFAQVRSSGASTPQVAFLCPFWDPPRVVRELYRDLYAPGIHPELWYRWEGKPLI
                     LADPNRLPIGEGNAAHDAPVALAVGHTLGQSFVTDKAVIAVGASCPTWKERDSAVTVT
                     LYRGGPAGEPLEARRFEAVRDNAWLMVETHPPLPPGSYYLELSDASGRIGWWSHSKDV
                     YAHGQAYADGAEVAGDRTLRLLAADDETTAIRDFFTFRTPEASYFAGPSRPDMWSWLE
                     VHPQHVFQNSRGEKEQMSVGVAQNAVGNRLGSMSEPDARGRSFHNGARDARPGAVNLG
                     LNFAEQWERALEEDPQFVFVTGWNEWIAMRFDEFAGIKAPVMFVDQFDQEHSRDIEPM
                     RGGHGDNYYYQLVAYIRRYKGVRRPPAAGPPVPIDLDGDFGQWTDVTPEFRDDIGDEA
                     RRDHPGYNHCAQYTNTTGRNDFVTLKVARDESLVYFYAQTREPITPHTDPHWMLLYID
                     ADSNSATGWQGFDFVVNRTVRDGEATVLEAREAEEWRERAAVPWRVSGRELMLAIPRT
                     ELGLNVPAESVRFRFKWADNTQRDDDTDEFILNGDAAPNGRFCYVYASEP"
     gene            2516..3313
                     /locus_tag="PLQ54_05250"
     CDS             2516..3313
                     /locus_tag="PLQ54_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS92697.1"
                     /translation="MVDRYGRVISYLRISVTDTCNLRCYYCAPPSGAPRASRDPLRAR
                     EIAEVARVAASLGITKFRITGGEPTLRSDLPQIVESLAELPGLTDLAMTTNGTRLAPL
                     AARLRDAGLRRVNVSLDSVDPARYREITGGGDLQQSLEGIAAAVGAGLFPLKLNCVVS
                     ASADEADARGVAAFAARHGYEARFIPRMDLASGRYSVVLGGDGGDCARCNRLRLTCDG
                     LVVPCLFSDLRYSVRELGARAALEAAVEHKPARGGPCGTGSLYDIGG"
     gene            3342..>4072
                     /locus_tag="PLQ54_05255"
     CDS             3342..>4072
                     /locus_tag="PLQ54_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS92698.1"
                     /translation="MSQTTGKGQMGRRQFLAKTASAVTAAITVVRPSAVRGADANGRL
                     ELGIIGTGGRGRWIGELFEQHGNAKVVAVHDYFQDRVDGARERFGLEAGRCHTGLDGY
                     RKLLDGPVDAVAIESPPYFHTAQVQASVEAGKHVFLAKPMAVDVPGCQTILAAGEKAK
                     GRLSFVVDFQTRNHPIFREAVRRVHMGAIGAPVLGQCFYHTGRLNPQAKPGTPEARLR
                     NWVFDVALSGDIIVEQNIHVIDVAC"
BASE COUNT          618 a         1301 c         1468 g          685 t
ORIGIN      
        1 aagaccaggc gggcgttccg cgcgaccttc gcgagaccca gccgcccggc ccagggccct
       61 atgttaccgc ccatgcccct tgggggctca gaccagcccg gctggacccc cgaaggggcg
      121 tagggggcgt tagcccgggg tagccagagg ccgttgctcc tcgatctctg gcccctaggg
      181 ttcgctggca tacacatagc agaaccgccc attgggcgcg gcgtcgccgt tcaggatgaa
      241 ctcatcagtg tcatcgtcgc gttgggtgtt gtccgcccat ttgaagcgga accgaactga
      301 ctcggccggg acattgaggc ccagttcggt ccgcgggatc gcgagcatca gctcgcggcc
      361 gctcacccgc cagggtaccg cggcccgctc gcgccactcc tctgcctccc gcgcctcgag
      421 tactgtggct tcgccgtcgc gcaccgtacg gttcacgacg aagtcgaagc cttgccatcc
      481 cgtggcggag ttggagtcgg cgtcgatgta cagcagcatc cagtggggat cggtgtgggg
      541 cgtgatcggc tcgcgtgtct gggcgtagaa gtagacaagg gactcatcgc gggccacttt
      601 gagggtgacg aagtcgtttc ggcccgtggt gttcgtgtac tgcgcgcagt ggttatagcc
      661 cgggtggtcg cggcgggcct catcgccgat gtcgtcgcgg aactccggtg tcacgtcagt
      721 ccactggccg aagtccccat cgaggtcgat ggggacggga ggaccggcgg ccggtggcct
      781 gcgaacgccc ttgtagcgcc ggatgtaggc gacgagctgg tagtagtagt tgtcgccgtg
      841 gccgcccctc atgggctcga tgtcgcggct gtgttcctgg tcgaactggt cgacgaacat
      901 cacgggggcc ttgatgccgg cgaactcatc gaagcgcatg gcgatccact cgttccatcc
      961 ggtcacgaac acgaactgcg ggtcttcctc gagtgcccgc tcccactgtt ccgcgaagtt
     1021 gaggcccagg ttcacggcgc cggggcgggc gtcgcgggcg ccattgtgga agctccgtcc
     1081 cctggcgtcg ggctcgctca tcgatccgag gcggttcccc acggcgttct gggccacgcc
     1141 gaccgacatc tgctctttct cgccgcgtga gttctggaac acgtgctgcg gatggacctc
     1201 aagccaactc cacatatcgg gcctgctcgg gccggcgaag tagctggcct cgggcgtccg
     1261 gaaggtgaag aagtcgcgga tggccgtggt ctcgtcgtcg gcggcaagaa ggcgcagagt
     1321 gcggtcaccg gcgacttccg caccatcggc gtaggcttgg ccgtgtgcgt agacgtcttt
     1381 cgagtggctc caccagccga tgcgccccga tgcgtcggac agctcgaggt aataggatcc
     1441 cggtggcaga ggtgggtgcg tctcgaccat gagccaggcg ttgtcccgca cggcctcgaa
     1501 ccgccgggcc tccagtggct ccccggctgg gccgccgcgg tagagggtga cggtcaccgc
     1561 cgagtcgcgc tccttccagg tggggcatga ggcgccgaca gcgatgacgg ccttgtcggt
     1621 gacaaagctc tgtcccagcg tgtgtcccac ggccagagcg actggtgcat cgtgggccgc
     1681 attgccctca ccgatgggca gccggttggg gtccgcgaga atcagcggct tgccttccca
     1741 ccggtaccac agttcggggt gaatgccggg ggcgtagagg tcccggtaga gctcgcgaac
     1801 cactcggggc gggtcccaga aggggcacag gaacgccacc tgcggcgtgc tcgccccgct
     1861 cgagcggacc tgggcgaacg catccaacag cgccaggtac cagcggcggt acgtaatctg
     1921 attggtcaca tcgaagatga tgacatcgat ccccgcatcg gagagcatct gcgcatgctt
     1981 gcgcagcacg taggcatcat cggtctggta gtagccgaag agcggctcgc cccagtggtg
     2041 gaaggcgtgc atggggcccc agagggggtg gtctttgtcg ttgatggcat tggggtcggc
     2101 ggcgaggatc ttgctgttgt cgaatggccc ctggcctccg gcatgctctc cgagccacag
     2161 gaagtagaaa atgcctactt ggcgtccggc gcgtggcggt ccgcattcat ggaagcccgg
     2221 gagctcccgc cccagggcgt ctgtggccgt ccacgtatcg ctgagtacgt cacgcggctg
     2281 ggcggacatg accgccgagg gcaacacggc aagggccgcg gcgagggcgg gacggtagag
     2341 ccgcgggaga gcggcgaagc gagggcatcg catcgggcag cggcctttcg gctggccggg
     2401 tcggccagag ggatccagcg ctcggtgtgg aaccgggccg tgagtgtacc acttcctggg
     2461 ttcggcggcg gccccttcac gggagggctc ggtccgggcc cggcggagga gcaccatggt
     2521 cgaccggtac ggacgggtca tcagctacct ccgcatctcg gtgaccgaca cctgcaacct
     2581 gcggtgctac tactgcgcgc cgccgtctgg cgcgcctcgg gcgtcccggg atccgctgcg
     2641 tgcgcgggag atcgccgagg tggcgcgggt ggctgcgagt cttgggatca cgaaattccg
     2701 catcaccggt ggcgagccga ctctgcgatc ggacctcccg cagattgtgg agagtctcgc
     2761 cgaactgccc gggctcaccg atctcgcgat gacaacgaat ggcacgcggc tcgcgcccct
     2821 ggcggcgaga ctccgtgatg ccgggctacg ccgggtcaac gtcagcctcg acagcgtgga
     2881 tccggcgcgt taccgcgaga tcaccggcgg aggcgacctg cagcaatcgc tggaaggcat
     2941 cgcggccgcc gttggggctg gcctgttccc gctgaagctc aactgcgtgg tgtccgcatc
     3001 ggccgatgaa gccgacgcgc ggggagtggc agccttcgcg gcccggcacg gctacgaggc
     3061 tcgcttcatc ccacggatgg acctggcttc ggggcggtat tcggtcgtcc tgggcggcga
     3121 cggcggggac tgtgcccgct gtaaccgcct tcggctgacg tgcgatgggt tggtggtccc
     3181 gtgcctgttc tcggacctgc ggtactcggt gcgcgagctg ggagcgcgcg cggctctgga
     3241 ggccgctgtc gagcacaaac cagcgcgcgg cgggccgtgc gggaccggtt cgctatatga
     3301 cattgggggc taggcgagcc cccgtcagag gagggactcg ggtgagtcag accacgggca
     3361 agggccagat gggccggcgg cagttcctgg cgaagacggc gagcgcggtg acggcggcca
     3421 tcaccgtggt gcggccgagc gcggtgcgtg gcgcggatgc aaacggccgt ctcgagttgg
     3481 gcatcatcgg caccggcggc cgcggccggt ggattggcga gctgttcgag cagcacggga
     3541 acgccaaggt ggtggcagtg cacgactact tccaggaccg tgtcgacggc gcccgcgagc
     3601 ggttcggcct ggaagccggg cggtgccaca cgggcctcga cggctaccgg aagctcctcg
     3661 atggcccggt cgacgccgtc gccatcgaga gcccgccgta cttccacacg gcccaggtgc
     3721 aggcatcggt cgaagctggg aagcacgtgt tcctggccaa gcccatggcc gtggacgtac
     3781 ccggttgcca gacgattctg gccgccggcg agaaggccaa gggtcggctc tcgtttgtgg
     3841 tcgatttcca gacgcggaac caccccatct ttcgcgaggc ggtccggcgc gtgcatatgg
     3901 gcgccatcgg cgcgcccgtc ctcggccagt gcttctacca caccggacgc ctcaacccgc
     3961 aggccaagcc gggcacgccg gaagcgcggc tgcggaactg ggtgttcgac gtggccctct
     4021 cgggcgacat tatcgtcgag cagaacatcc acgtcatcga cgtggcctgc tg
//
LOCUS       DAOSPL010000210         4065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22155_length_4065_cov_3.402494, whole genome shotgun sequence.
ACCESSION   DAOSPL010000210 DAOSPL010000000
VERSION     DAOSPL010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22155_length_4065_cov_3.402494"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..1058
                     /locus_tag="PLQ54_05260"
     CDS             249..1058
                     /locus_tag="PLQ54_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92699.1"
                     /translation="MPRYAPADPPEDTLGGYFRAAITHHLRTWVWVTEAGEAALCIYL
                     IALGAGDMTPEVFMLYPGIFALVVAGTLAYRRTIACRSERSYVDLGEVRNRQALLFGE
                     ALACFLMSAILVAVTFVAGAVLAPGKIGTAGALAVGYGLILINAATAVMVTFLFSGLV
                     LSSDAPSVLSIVVLVLGASRSDLSREIMRSVPLEPLVFVSRLLTPPFEETIKCAMHGT
                     LALDWAYAVQTVIYGCLAACISSALFARRALAGGRTVPVRAPRTKKEPHAS"
     gene            1196..2782
                     /locus_tag="PLQ54_05265"
     CDS             1196..2782
                     /locus_tag="PLQ54_05265"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOS92700.1"
                     /translation="MTPLPGEVTAAGSVRTERDYLDSSLLVVRFTPGGAEVTLTSEAG
                     EELGRAIDRGSDAYWTAFRLFGVPRAGLPCVVRLAKPGFADASRTVTVPPRSLVHADF
                     VLARASGLERIAYCRESGQTADIWWAWPDGTHAEPLVATGDDERDPCFTPDGRALAYA
                     AGPPGGRRIMLLDLVSREARTLVDAGGDQHSPCFSPDGRLLAFVSDREGDPDIFVAAA
                     DGSGGRRVSRRGATDASPSFAPDGKQIVFATDRWGSMDLAIVSLGTGRIERLTDDPRD
                     ETDPAWSPDGETIAYTLRAGSRPILKLRSSRSPLGVGPLDDGLPARVALGASQPAWSP
                     DSRVILSSVVDGDRRRVLGLRMRTDWMIPVTPETERCSDATWGRLPTPEEGLPRLRVR
                     TSRGDIVIELDVARAPATVANFLRLVDAGFYNGLSVYRVSPGQLVQLGCPLGNGTGGP
                     AWTLPFENTGLPHHRGAISMVHGDDPNGGGSQFMICLTDLPALDGSFAAFGRVVQGLD
                     TAETLQVGDTIAGIAATNGE"
     gene            complement(2797..3438)
                     /locus_tag="PLQ54_05270"
     CDS             complement(2797..3438)
                     /locus_tag="PLQ54_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92701.1"
                     /translation="MSELECTQCGAVVQRFDLYCPSCQSDLMEAGATRRVELGQESPQ
                     PTPEAGEPLAAEPRSLYATPVKDPFVRRLAPAEPETDPFANQPPFMIQGLAAGTICGV
                     VFGAIAALLMWLMGSNARTAGIAQALLALLGFLPWMFSGVIIGLTMGFTQDFSMSGAA
                     WAIWPIAVVYSLLATMVMGTAYALMPTLMFALAEGYMVGWVRGLIWAPSWDVG"
     gene            complement(3629..>4065)
                     /locus_tag="PLQ54_05275"
     CDS             complement(3629..>4065)
                     /locus_tag="PLQ54_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92702.1"
                     /translation="TVTLQIPSAYECTVAQAAAADHEMIVNTPAVSSGHPFEIDLSPI
                     GDVGRGSLVVHTALSPASTMTTRGTTLTIGVKGSTRSSCARFGVVTSTETIAANASSG
                     TITIQFTATGFTGTATLTTEDQRVPAGLRTYTTAFQGELPED"
BASE COUNT          599 a         1433 c         1369 g          664 t
ORIGIN      
        1 atgcgtcggc gccgctggcg ggggagtttg aggtgctctc acccgagatg ccgccgccgg
       61 ggcccagcgg gctgtaggct ggcactcggt cgcgcccggg atatcccggt gtgtggctcc
      121 gactgtgtga tccgagcctc gcggcgcgtt gacagctcgc ggcgcggcgc ggtacactcg
      181 cgccaacaca gggggcctcg gggttccggg gctcccgtgg ttttgccccc tcttgagtga
      241 ggaccttcat gccgagatac gcgcctgccg atccacccga ggacaccctc ggaggttact
      301 tccgtgccgc catcacccac catctgcgca cctgggtatg ggtgaccgag gcgggcgagg
      361 cggcgctgtg catctacctc atcgccctcg gcgccggcga catgaccccg gaggtgttca
      421 tgctgtaccc ggggatcttc gcactcgtcg tcgcggggac cctggcttac cgccggacca
      481 tcgcgtgccg gtccgagcgg tcctatgtcg acctgggaga ggtgcgcaac cgccaggccc
      541 tgctgttcgg cgaggctctg gcctgcttcc tgatgtccgc catactcgtc gcggtcacgt
      601 tcgtggccgg cgcggtcctg gcacccggga agatcggcac cgcgggggct ctcgccgtcg
      661 ggtacggact gatcctgatc aatgccgcca ccgcggtgat ggtcacgttc ctgttctccg
      721 gccttgtgct ctcgagcgat gccccctcgg tgttgtctat cgtggtgctc gtgttgggcg
      781 ccagccggtc cgatctctcg agagagatca tgcggtcggt gcccctggag cctctggtct
      841 tcgtcagccg cttgctgacg cctccgttcg aggagaccat caagtgtgcc atgcacggga
      901 ctctggccct cgactgggcg tacgcagtac agacggtgat ctacggatgc ctggcggcct
      961 gtatctcgtc tgcgctcttt gcgcgccggg cccttgccgg cggacgcacc gtgcccgtgc
     1021 gcgcgccgcg cacgaagaag gagccccacg cgtcgtagcg ttacgccccg cccgtggagc
     1081 cgtcgcgcga cggacgtatg cgtgcgttgg cctcgtgcta tgtctgatct cgctggtacc
     1141 gcgccccggc tggtgtcagg ccgctggagg gcccggccgg gggctgcctc tgccggtgac
     1201 cccgctgccc ggtgaggtca ccgccgccgg ttcggtccga acggagagag actacctcga
     1261 ctcatcgctc ctggtcgtgc gcttcacgcc cggcggggca gaggtgacgc tcacttcgga
     1321 agccggcgag gagctggggc gggccattga ccgcggcagc gatgcctact ggacggcctt
     1381 ccgcctcttc ggggtacccc gggccggact gccgtgcgtc gtgcgcctcg ccaagcctgg
     1441 cttcgccgat gcgagccgca ccgtcacggt cccgccgcgt tcgctggttc acgccgactt
     1501 cgttctggcc cgggcgagcg ggctggagcg tatcgcgtac tgccgcgagt cgggccagac
     1561 ggccgacatc tggtgggcgt ggcccgatgg aacccacgcg gagccgctcg tagccaccgg
     1621 cgacgacgag cgcgatccct gtttcactcc cgacgggcgg gcactggcct atgccgcagg
     1681 cccacccggt gggcgccgga tcatgctcct cgacctcgtc tcccgcgaag cgcggacgct
     1741 cgtcgatgcc ggcggcgatc agcactcccc ctgcttctcg cccgacggac gactcctggc
     1801 tttcgtcagc gaccgtgagg gtgaccccga catattcgtg gccgccgcgg acggctcggg
     1861 cggtcgccga gtcagccgaa gaggggccac ggacgcgtcg ccgagtttcg cgcccgacgg
     1921 caaacagatc gtcttcgcca ccgatcggtg ggggtccatg gacttggcca tcgtgtcgct
     1981 cggcaccgga cgaatcgagc gcctcaccga tgacccgcgc gacgagactg acccggcgtg
     2041 gtcgcccgat ggcgagacca tcgcgtacac cttgcgcgcc ggatcgcgcc cgatcctgaa
     2101 gctgcgctcg tctcgctcgc cgctgggcgt cggcccactc gacgacggac tcccggctcg
     2161 agtggcgctg ggcgccagtc agccagcctg gtcaccggac tcgcgagtca tcctgagctc
     2221 tgtggtcgac ggagaccgtc ggcgcgtgct cggcctccgg atgcggaccg actggatgat
     2281 cccggtgacc ccggagaccg aacgctgcag cgatgccacc tggggccgcc tcccaacgcc
     2341 tgaggaaggc ctgcctcgcc tgcgggtgcg gaccagtcga ggcgacatcg tcatcgagct
     2401 cgacgtcgca cgcgcgccgg ccacggtcgc caacttcctc cgcctcgttg acgccgggtt
     2461 ctacaacggg ctatcggtgt accgggtgag ccccggccaa ctcgtccagt tgggctgccc
     2521 gctgggcaac gggaccgggg gaccggcgtg gacgctcccg ttcgagaaca cgggactgcc
     2581 ccatcaccgg ggcgccatct cgatggttca cggcgatgat cccaatggtg gcgggagcca
     2641 attcatgatc tgcctgaccg acctgccggc cctggacggc agcttcgccg cattcggccg
     2701 cgtggtccag ggcctcgaca ccgccgagac cctccaggtc ggcgacacca tcgccgggat
     2761 tgccgccacc aacggcgagt gagcccgggt ccgcgcctag cccacatccc agctcggcgc
     2821 ccagatcaac ccccgcaccc agcccaccat gtacccctcg gcgagggcga acatcagtgt
     2881 cggcatcagg gcgtaggccg tgcccatgac catggtcgcc agaagcgagt agaccacggc
     2941 gatcggccaa atggcccacg ccgcgcccga catgctgaaa tcctgggtga accccatggt
     3001 cagcccgatg atcacgccgc tgaacatcca tggcaggaac ccaagaaggg cgagcagggc
     3061 ctgggcgatc cctgccgtac gggcgttcga gcccatcagc cacatcagca acgccgcgat
     3121 agccccaaag accacgccgc agatggttcc cgcggccagg ccctggatca tgaatggcgg
     3181 ctggttcgcg aagggatccg tctcgggctc cgccggagcc agtcggcgca caaatgggtc
     3241 cttgacgggg gtggcataca gggatcgagg ttcggcggct agaggctcac cagcctcggg
     3301 tgtcggctgg ggagactcct ggcccagctc caccctgcgc gtggcgccgg cctccatcag
     3361 gtccgactgg catgagggac aatagaggtc gaaccgctgc accaccgcgc cgcactgtgt
     3421 gcactcgagc tcggacatga gacagccccc ccggcagcga gcgtacccgg cgacaagtcg
     3481 gaagccgggg acggcccagt caggcactca gtgcaatgcc ggactcgagg caacggcgcc
     3541 gcccactccc gggtgcggca cccatacaaa cggccgtccc cgcgatcggg ggcggccggt
     3601 gtcgtctgac ctggactcgc gcgcagccct agtcctcagg aagctcgccc tgaaacgcgg
     3661 tcgtataggt cctcagacct gcggggaccc gctggtcctc tgtggtcagt gtggcagtgc
     3721 cggtgaaccc ggttgcggtg aactggatcg tgatcgtgcc cgaggaggcg ttagccgcga
     3781 tcgtctcggt gctggtcacc acgccaaagc gggcacaaga cgatcgcgtg ctacctttca
     3841 ccccaatggt gagcgtggtg ccacgcgtcg tcatcgtcga ggccggtgag agcgccgtgt
     3901 gaacgacgag ggaaccccgc cccacatcac cgatcggact caggtcgatc tcgaacggat
     3961 gcccactgct cacggcaggc gtgttcacga tcatctcgtg gtcagctgcc gccgcctgag
     4021 ccaccgtgca ctcgtaggcc gacgggatct gcagggtgac cgtcg
//
LOCUS       DAOSPL010000211         4054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22232_length_4054_cov_3.262816, whole genome shotgun sequence.
ACCESSION   DAOSPL010000211 DAOSPL010000000
VERSION     DAOSPL010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22232_length_4054_cov_3.262816"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLQ54_05280"
     CDS             <1..560
                     /locus_tag="PLQ54_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOS92703.1"
                     /translation="LLLARERARANAVAGPGDLVSLAAVIGAVVELLEARAQASGVAL
                     ATDLPPDLPAIRADREALTQVFTNLIGNGIKYTPEGGAVRVSARAEDGAVAVCVADTG
                     IGIPAEDVPRVFEEFFRASNARSHAAVGTGLGLAIARSAVEALGGSIRVSSQEGVGTQ
                     FTVTLPIEPSQGPLAVSGLAPSPPE"
     gene            820..2844
                     /locus_tag="PLQ54_05285"
     CDS             820..2844
                     /locus_tag="PLQ54_05285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOS92704.1"
                     /translation="MSWLRRRAPTIALGVILIAVTVTAEMTHRQPSKPRASKSEAVTP
                     VPVQTAEAKLRRLPRTVEVSGQVEPLVRAEVAPEIMGRILEMGPREGDQVSEGTVLAR
                     IDDSAAQAEVARANAMVAAARAAKSQAEAAFKIQQTQSEVRVSQAQQDLAMAQSSREE
                     EKAQADEAVRQAESAVAAAQALVDLVKEGARKQEKAQAQERVRQAQAGLEQARAALDK
                     ARNGPRPQEVKEAEHAVAQAQSAVDQAEAALSKAREGARRQERAEADQSVILAEAQYT
                     TAEAAHRRMKALMDAEVISKQAFEESELKLQAAKSALEVARQRTNLVHEGARTQDIQS
                     AEAAVSQAKAAHSAALERLKLLQEGTRPEDVRMAEQQVAAAESAVEQARQHAEMLLEG
                     PRSQEVIQAEEALRQAEAQAEIARKARDMVHAGARPEKVKQAEDAVRMAEASTEENTI
                     RQQAVEAAQAALAQAEAAAQAANVTLAHTQVRCPYTGIVVERLADAGDMAVPGQPIVV
                     IQPNDRFQLRCDVPEAEAKLLQTNQRIDVVIDAIGAKPLSATVVTVTPSTDASSRSSV
                     VKLALPADPRLRSGQFGRARIPIGQRPGIVVPIAAVRRHESVTSVFVVDRGFARSRLV
                     RIGEDRGKQIEIIAGLEGGEQVIVSDVERLVDGIPVKTGQASPRAKRAEQ"
     gene            2852..>4054
                     /locus_tag="PLQ54_05290"
     CDS             2852..>4054
                     /locus_tag="PLQ54_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOS92705.1"
                     /translation="MSDQERPAGEGGHVQGEVRSGPGRHRYGLAGATAHMFIDSKLTP
                     LVVFASLMLGAMAALLIPREEEPQIIVPMVDIFVQMPGASAEEVEQRVTKPMEKLLWE
                     IPGVEYIYTTSSSGQCMAIVRFRVGQDEEDSLVRLCNKLYSNYDLIPPGCSEPLVKLR
                     SIDDVPVLALTLWTDAPYYERGGQVLRRVAAQLEDDLKEVPDVSSTRIIGGDRRQICV
                     RADRVRLAGHGVDLDRLIQALRGANRELPSGSFSAQDYRFQVDTGEFLKSAADVESVV
                     VTVGPGGPVYVRDVADVSDTGEEPSSYVYFGLGPRSEEKGVRPIGYDRTFDAVTISVA
                     KRKGTNAATVAHQVLRKVKTLRGAAYPSDVHITVTRNYGETALEKANELLKHLLIATL
                     SVAVLIAFA"
BASE COUNT          670 a         1334 c         1467 g          583 t
ORIGIN      
        1 tgttgctgct ggcgcgagag cgggcgcgcg ccaacgccgt ggccgggccc ggcgatctgg
       61 ttagcctcgc cgcagtgatc ggggcggtgg tggaactcct ggaggcgcga gcccaggcga
      121 gtggcgtggc gcttgcgacg gacctgccgc ccgacctgcc ggcgatccgg gccgatcgcg
      181 aggccctaac acaggtgttc accaacctga ttggcaacgg catcaagtac acgcccgagg
      241 ggggagcggt gcgcgtgtcg gctcgtgcgg aggacggtgc cgtggcggtt tgtgtcgccg
      301 acacggggat cgggatcccg gccgaggacg tgccgcgggt atttgaggag ttcttccgtg
      361 cctcgaatgc ccgaagccat gccgcggtgg gcacgggcct gggcctcgcc atcgccagga
      421 gcgccgtcga ggccctgggc ggatcgatac gggtctcgag ccaggaggga gtcggcaccc
      481 agtttaccgt caccctcccg atcgagccct cgcagggtcc cctggccgtc tccggtctgg
      541 ccccctcgcc ccctgaatga ggggtcggcg gacacccttt cgcactctca ggaaggtatc
      601 agggcccgag ggctcggaaa taggcgctag ttcccggctg tcggcggagc ccccctgggg
      661 cggcccgccc cacgtggggc cgccgttatg ggggcccgcg ttgtgttacc gcccccgcgc
      721 ctccgcgaaa cggtggggcg cctcgcttgc cgtcagccgt gatgcgtgcg ggacgggccg
      781 tgcccggccc gtgcctgcat ggagggagtc gcggccatca tgtcctggct acgacggcgc
      841 gcgccgacga tcgctctggg ggtcatcctc atcgcggtga cggtcacggc cgagatgact
      901 caccggcagc catcgaagcc ccgggccagc aagtccgagg cggtaacgcc tgtccccgtg
      961 cagaccgccg aggcgaagct ccgcagattg ccgcgtacgg tggaggtctc gggccaggtc
     1021 gagccgttgg tgcgggccga ggtggcgccc gagatcatgg gccggattct cgaaatgggg
     1081 ccgcgcgagg gcgaccaggt gtcggagggg actgtgctgg cgcgcatcga cgacagtgcg
     1141 gcccaggccg aggtcgcccg ggccaacgcc atggtcgctg cggcccgcgc agcgaagtcc
     1201 caggccgaag ccgcgttcaa gatccagcag acccagagcg aggtgcgggt ctcgcaggcc
     1261 cagcaggacc tcgccatggc gcagagctcc cgcgaggagg agaaggccca ggcggacgag
     1321 gccgtgcgcc aggccgagtc ggccgtggcg gccgcccagg cgctcgtcga cctggtcaag
     1381 gagggcgcac ggaagcagga gaaggctcag gcccaggaac gggtgcggca ggctcaggcc
     1441 gggctggagc aggcccgtgc tgccctggac aaggccagaa acgggccgcg tccccaggaa
     1501 gtcaaggaag ccgagcacgc ggtggcccag gcccagtcgg ctgtagacca ggccgaagcc
     1561 gcgctcagca aggcgaggga gggcgcacgg aggcaggagc gggccgaggc cgatcagtcg
     1621 gtgatcctcg ccgaggccca gtacaccacg gctgaggccg cccaccggcg catgaaggcc
     1681 ctgatggacg ccgaggtgat ctcgaagcag gcgttcgagg aatctgagct taagctacag
     1741 gctgctaagt ccgcactgga agtcgcccgc cagcggacca acctggtgca tgagggtgcc
     1801 cgcacacaag acatccaatc ggcggaagcg gcggtgtctc aggccaaggc ggcccacagt
     1861 gcggctctgg agcgcctcaa gcttctgcag gagggcacgc gacccgagga cgtccggatg
     1921 gccgagcagc aggtcgccgc cgccgagtcg gcggtcgaac aggccagaca gcatgccgag
     1981 atgctgctcg aaggcccccg ctcgcaggag gtcatccagg ccgaggaggc gctgcgccag
     2041 gcggaggcac aggccgagat cgcgcgcaag gctcgcgata tggtgcacgc gggagctcgg
     2101 cccgagaagg tcaagcaggc ggaggacgcc gtgcgcatgg ccgaggcgtc cactgaggag
     2161 aacaccattc gtcagcaggc ggtcgaggca gcccaagcgg ccttggcgca agccgaggcg
     2221 gcagcccagg ccgccaacgt tacactggcg cacacgcagg ttcgatgccc gtataccggg
     2281 atcgtggtcg agcggctcgc cgacgccggg gacatggcgg tgcccggtca gccgatcgtg
     2341 gtgattcagc ccaacgatcg attccagctc cgctgcgacg tccccgaggc cgaggcgaag
     2401 ctcctccaga ccaaccagcg catcgacgtg gtgatcgacg ccatcggcgc gaagccactg
     2461 tcggccaccg tggtgactgt gaccccgtcg acggatgcct cgagccgatc ctcggtggtg
     2521 aagctggcgc tgccggccga tccgcgcctg cgctcggggc agttcggccg tgcccgcatc
     2581 cccattggcc agcggccggg catcgtggtg cccatcgcgg ccgtgcgccg gcatgagtcg
     2641 gtcaccagcg tgttcgtggt ggaccgcggt ttcgcgcgca gtcgcctcgt gcgtatcggc
     2701 gaggaccgcg gcaagcagat cgagatcatt gccggcctcg agggcggcga gcaggtcatc
     2761 gtgtccgatg tcgagcggct tgtcgacggc atcccggtca agacgggaca ggccagcccc
     2821 agagccaaac gggcggagca gtgatggcag cgtgagtgac caggagcgcc ccgccggcga
     2881 gggggggcac gtgcagggcg aggtgcggtc cgggccggga cggcatcgct atggcctggc
     2941 cggcgccacg gcccacatgt tcatcgactc gaagctcact cccctggtgg tcttcgcgtc
     3001 gctgatgctt ggcgccatgg ccgcgcttct catcccccga gaggaagaac cccagatcat
     3061 cgtccccatg gtcgacatct tcgtgcagat gccgggggca tcggccgagg aggtcgagca
     3121 acgggttacc aagcccatgg agaagctcct ttgggagatc ccgggggtcg agtacatcta
     3181 cacgacatcg agctctggcc agtgcatggc cattgtgcga ttccgcgtcg ggcaagacga
     3241 ggaggacagc ctcgtccggc tctgcaacaa gctttactcc aactacgacc tcatcccccc
     3301 gggctgttcc gagcctctgg tgaagctccg ttccatcgac gatgtgcccg tgctggctct
     3361 gacgctgtgg accgatgctc cgtactatga gcgcgggggc caggttctgc ggcgggtggc
     3421 cgcccagctg gaggatgacc tgaaggaagt cccggacgtc tcgtcgacgc ggatcatcgg
     3481 tggggaccgc cggcagatct gcgtccgcgc ggaccgcgtg cggctcgccg gacacggcgt
     3541 cgatctggac cggctgatcc aggcactccg cggcgccaac cgagagctac catcgggttc
     3601 cttctcggcc caggattacc gcttccaggt cgacacaggc gagttcctga agtcggcggc
     3661 cgatgtggag tcggttgtgg tcaccgtcgg gcccggtggg ccagtctacg ttcgcgacgt
     3721 ggcggacgtc agtgacaccg gcgaggagcc gagcagctat gtctacttcg gcctgggccc
     3781 aaggagtgag gagaagggcg ttcgccccat cggctacgac cgtaccttcg acgccgtgac
     3841 catatccgtg gccaagcgca agggcaccaa tgcggctacc gtggcccacc aggtgctgcg
     3901 caaggtcaag accctccgtg gcgcggccta tccctcggat gtgcacatca ccgtcacccg
     3961 caactacggc gagacggccc tcgagaaggc caatgagcta ctgaagcacc tgctgatcgc
     4021 cacgctgtcg gtagcggtac tgatcgcctt cgcg
//
LOCUS       DAOSPL010000212         4052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22250_length_4052_cov_2.680761, whole genome shotgun sequence.
ACCESSION   DAOSPL010000212 DAOSPL010000000
VERSION     DAOSPL010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22250_length_4052_cov_2.680761"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PLQ54_05295"
     CDS             complement(<1..335)
                     /locus_tag="PLQ54_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOS92706.1"
                     /translation="MAEHRWSRRGFIRAAGTAAAASALPLSVVEMAFADPARNFRIAY
                     ISDAHIQHIRGTQFVRNWDRGLIRAVAECNLLTPAPDFVVFGGDLAQLGTKEELDHGA
                     EMLSALKYKT"
     gene            complement(328..1488)
                     /locus_tag="PLQ54_05300"
     CDS             complement(328..1488)
                     /locus_tag="PLQ54_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014893007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HOS92707.1"
                     /translation="MRGNSIRWLLAAGVLACAAAVGADQALAPAPLGLPALRVPADNP
                     QTPEKIALGKKLFEDKRFSSTGEVACANCHMAEKAFTDGPLKVSEGINKLTGTRNAPT
                     VINSAYMRSMFWDGRSPTLEDQALHPFVNPVEMGLKDHEPILRIVRSDPEYTRAFGRV
                     FDKSGNKITMTEVTKAIAAFERTIISGNSPFDRYYFGHDAKALTPAQIRGLDLFVNKA
                     RCVSCHVIEQTQALFTDNRFHNVGVGINDIQDDAPALATEFLRAKATLSEVDVKVLSD
                     KRTSELGRLAVTRSLEDIGSFKTPTLRNVAVTAPYMHDGSKATLRDVIVHYNNGGVTN
                     EGDRVNDFLSSGIRPLNLTEDEIDDLVAFMEALTSPEFADLPKATEGGAGNG"
     gene            complement(1616..2011)
                     /locus_tag="PLQ54_05305"
     CDS             complement(1616..2011)
                     /locus_tag="PLQ54_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOS92708.1"
                     /translation="MDILGLLRSHGIQPTPQRMAVAGFAIDSRNHPSAEEVYLGVREA
                     CPTVSRATVYNTLHLLVERGLVRQLALREGVCVYDSQTAPHHHFIDDDTGRIEDVPWD
                     AVSVTGTDTLQGIDVREFQVVMRGRRRPR"
     gene            2124..3695
                     /locus_tag="PLQ54_05310"
     CDS             2124..3695
                     /locus_tag="PLQ54_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOS92709.1"
                     /translation="MRHWRPISRRAALRDLGALGAAVALGEPHGAFPHADGEVRPVPP
                     NLVLFLVDDMGWQDTSVPFHSSPTPFNDRYHTPSMERLADGGVCFTQAYACCVCSPSR
                     VSLMTGMNAARHRVTNWTLWKDRATDGAQGELMLPEWNVNGIAPLGGDPRAVHARCLP
                     AILRHRGYRTIHVGKAHFGAVGTPGADPRSLGFDVNIAGHAAGGPGSYLGTQNFSGRW
                     RGAEAVWDVPGLEHYHGQDIFLTEALTLEAIREMDAAVRRRQPFFLYLSHYAVHVPFA
                     DDPRFVGKYDRAGLDHTEAQYAALLEGMDQSLGDILDRLRYHGIEQNTVVLFMSDNGG
                     LSAYGRGGEPHTHNRPLSSGKGSAHEGGIRVPMIVSWPGIVRPGTTCAHPVIIEDFYP
                     TLLELAGVPEITSPDAQSVVPLLRGEAPGTGSRALHWHYPNCWGPPGPGIGASSTIRR
                     GDWKLIYYHADQSYELFNIAEDIGEERNRARSEPHRVSELADALRDHLLAVDAQMPSL
                     RSTGEPVPLPGESVP"
BASE COUNT          645 a         1295 c         1372 g          740 t
ORIGIN      
        1 gtcttgtact tgagcgccga gagcatctcg gcaccgtgat ccagctcctc tttggtaccg
       61 agctgtgcca ggtcgccgcc gaagaccacg aagtccggcg cgggcgtgag caggttgcac
      121 tccgccacgg cgcgtatcag accccggtcc cagttgcgga cgaactgtgt gccgcggatg
      181 tgctggatgt gggcgtcaga gatgtacgcg atccggaagt tgcgggccgg gtcggcgaaa
      241 gccatctcga cgacgctcag tggcagcgcg ctggcagcag cggctgtacc ggccgcgcgg
      301 atgaagcctc gacggctcca gcggtgctca gccattgccc gcacctccct cggtggcctt
      361 tggcaggtcc gcgaactccg ggctcgtcag cgcctccatg aaagccacca agtcatcgat
      421 ctcgtcttcg gtcaggttga gtgggcggat gccgctgctc aggaagtcgt tgacgcggtc
      481 gccctcattc gtcacgccgc cgttgttgta gtggacgatc acgtcacgga gcgtcgcctt
      541 gctgccatca tgcatatagg gcgcggtcac cgcgacgttg cgcagtgtcg gcgtcttgaa
      601 cgagccgatg tcctcgagcg agcgggtcac cgcgagccgg cccagctccg aggtgcgctt
      661 gtcgctcagc accttgacgt cgacctctga gagggtcgcc ttggccctca ggaactcggt
      721 cgcgagcgcg ggcgcgtcgt cctggatgtc gttgatgccg actccgacgt tgtggaagcg
      781 gttgtcggtg aacagggcct gcgtctgctc gatgacgtgg cacgagacgc atcgggcctt
      841 gttcacgaac aggtcaagcc cgcggatctg ggccggcgtc agagccttgg cgtcgtgccc
      901 gaagtagtag cggtcgaagg gcgaattgcc ggagatgatg gttcgctcga aggcggcgat
      961 ggccttggtc acctctgtca tagtgatctt gttgccgctc ttgtcgaaca cccgtccaaa
     1021 tgccctcgtg tactccgggt ccgaccgcac gatcctcagg atgggttcgt ggtctttgag
     1081 gcccatctcg accgggttca cgaacggatg cagggcctgg tcctcgagcg tgggcgaccg
     1141 tccatcccag aacatgctgc gcatgtatgc cgagttgatg accgtggggg cattgcgcgt
     1201 ccccgtgagc ttgttgatcc cctcagagac cttcaggggc ccatcggtga acgccttctc
     1261 ggccatatgg caattggcgc aggcgacctc acccgtactg ctgaagcgct tgtcctcaaa
     1321 cagcttcttt ccgagcgcga tcttctcggg cgtctgtgga ttgtcggcgg gcacccgcaa
     1381 ggcgggcaag ccgagcggcg ccggggcgag tgcctgatcg gcacctaccg ccgcagcgca
     1441 ggccaggact cccgccgcca ggagccatcg gatcgagttc cctctcatcg ctcctcctct
     1501 ggcatgtgcg gcgccgcaga gcgacgcctc atctcacggt actgcgctac ccagccccag
     1561 accaccggcg ggctggagac cggcgcccaa cccacgcaca ttcgggcgcg gccccctagc
     1621 gggggcgcct ccttccgcgc atgactacct gaaactcgcg gacatcgatt ccttgcagtg
     1681 tgtcggtgcc cgtcacagag actgcgtccc atggcacatc ctcaatacgt cccgtgtcgt
     1741 cgtcgatgaa gtggtgatgt ggagcggtct gagagtcgta cacacacacg ccctcgcgca
     1801 gcgccagctg tcgtacgagg cccctctcga ccagcaggtg gagggtgttg tagacggtgg
     1861 cgcgcgacac ggtggggcaa gcctcacgga cccccaggta gacctcttca gccgatgggt
     1921 ggttccggct gtcgatcgcg aaccccgcca cggccatacg ctgtggcgtg ggttggatcc
     1981 cgtggctgcg gagcaggccc aggatgtcca tggcgactct agaatgcctt cagatcagga
     2041 acatgtcaag gcgcgccgcc ctggcgcccg ttccccaggt gagcactggg cggtacccga
     2101 acactgtggg ggagggttct gtcatgcgcc attggcgtcc catatcgcgg cgagcggcgc
     2161 tgagggatct gggagccctg ggagccgcgg tcgccctggg cgagccgcat ggggctttcc
     2221 cacacgcgga tggcgaggtg aggccggtgc caccgaacct ggtgctgttc ctggttgacg
     2281 acatgggctg gcaggacacg tcggtgccct ttcacagctc gcccacgccg ttcaacgatc
     2341 gctaccacac gccgagcatg gagcgactcg ccgacggcgg cgtatgcttc actcaagctt
     2401 acgcttgctg cgtgtgctct ccgagccgtg tctcgctgat gacggggatg aacgcggcgc
     2461 gccatcgggt caccaactgg acgctgtgga aggaccgcgc gacggatggg gcccaaggtg
     2521 aactcatgct gcccgagtgg aacgtgaacg ggatcgcacc gctgggcggg gacccgcggg
     2581 ccgtccacgc ccgatgcctc ccggctatcc tccgccatcg agggtaccga acgatccatg
     2641 tgggcaaggc gcactttggc gccgtcggca cgccgggggc cgatccgcgg agtctggggt
     2701 ttgatgtgaa catcgccggc catgcggcgg gtgggccggg gagctatctg ggcacgcaga
     2761 acttcagcgg ccgctggcgc ggggccgagg cggtgtggga cgttcccggg ctggagcact
     2821 accacggcca ggatatcttc ctcaccgagg cgctgaccct ggaggcgatc cgcgagatgg
     2881 acgccgccgt gaggcggcgg cagcccttct tcctgtatct atcgcactac gccgtacacg
     2941 ttccattcgc cgacgatccg cggttcgtgg ggaagtacga cagagctggc ctggaccaca
     3001 cggaggcaca gtatgcagcc ttgctcgagg gcatggacca gtctctgggg gacatcctgg
     3061 accgcctgcg gtaccacggg atcgagcaga acaccgtcgt gctgttcatg tcggataacg
     3121 gaggtctgag cgcttacggc cggggtggcg agcctcatac tcacaacagg ccgctatcga
     3181 gcggcaaggg gtcggcacac gaggggggca ttcgtgtgcc catgatagtt agctggccgg
     3241 gcatcgtgcg gcccggcacc acctgcgcgc atccggtgat catcgaggat ttctacccca
     3301 ccctactcga gcttgcgggt gttccggaga tcacttcgcc ggacgcgcag agcgtcgttc
     3361 cccttctccg cggcgaggct cccggcacgg ggtctcgtgc actgcattgg cactacccca
     3421 actgctgggg gccgcccggg cctggcattg gcgcctcgag cacgatccgt cggggcgact
     3481 ggaagcttat ctactaccac gccgaccagt cctacgagct gttcaacatc gcggaggaca
     3541 tcggcgagga gcgcaacaga gcgcggtccg agccccaccg tgtatccgag ttggccgacg
     3601 cgctacggga tcacctcctg gcagtcgatg cccagatgcc gtcgttgagg tcgacagggg
     3661 aaccggtccc gctaccgggg gagagcgtgc cgtaactggg tggccgctgc ggcgtcgctc
     3721 cgtgtgagag gcaggcgagt cctgatggcc tggtggtgac ccttcgacgc ctgggactac
     3781 tggcatcttt acatcgtggt catcgtcggg cgaggggagc gcaagcgggt ccagcgccgc
     3841 acgcagtcga tgcaggtcag gcacaggaac gggccgcctc gccggagcgg ggcggcccgt
     3901 tcaccattgg ttgttccgtc tgccccggga ttacgagggg cgggtccagt agacgagctt
     3961 gctcgtctcg gcagcactgt ttgggtagat ggcctcgggc ttgctccact tcacgtggcc
     4021 gtccaggtag ccgcagttca tgccgtcgtt at
//
LOCUS       DAOSPL010000213         4050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22274_length_4050_cov_3.081602, whole genome shotgun sequence.
ACCESSION   DAOSPL010000213 DAOSPL010000000
VERSION     DAOSPL010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22274_length_4050_cov_3.081602"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PLQ54_05315"
     CDS             <1..643
                     /locus_tag="PLQ54_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92710.1"
                     /translation="YFCTPSLYLGGGEYQSAMSNAYRGLFFLDAPVGFVTDKMLAEGR
                     ADGLKVVVLPACRWVERESLDRLRALAARGVRLVTLGDGPTFDEYRHPHPAGSLLPAD
                     EVTSVRPGPPGDIRRQMAPILDRAQVRRPIRCFRADGETPEAVECRSVVHGDGVLCSL
                     LNLGKAPVRIRLEHHGETVRSWKDLISDRESDSGWMELRPSDPRLLWIAADRT"
     gene            809..2581
                     /locus_tag="PLQ54_05320"
     CDS             809..2581
                     /locus_tag="PLQ54_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOS92711.1"
                     /translation="MGNVELTINGVRVEADSGSTIMDAADKAGIHIPRLCHHPRLSMQ
                     GACRVCIVEVEGVRNTVASCCYPVRDGMVVRTHTPEIQRLRRDIVELLLDNHPSDCNT
                     CERNGNCELQRLASAMGIRERLFAGERKRYEKDLSSPSVIRDPEKCILCGRCIRVCGE
                     IQEDAALSQVYRGFDTVVMPAHNAGFMETVCVSCGQCINVCPTAAFLEKDATAPFMKA
                     IADPDTIVVSQMAPSVRAAIGECFGLQPGRAWEGQCFAAQRALGIDYVFDTQFSADLT
                     IMEEAAEFVAKVKAGGPLPNITSCCPTWVKYAEHFHPDMLKYLSSAKSPMSMQGALVK
                     TYWAEKMGVDPDRIFSVAIMCCTCKKFEATRPELHVDGRAAVDAVLTTREFGWLIKHM
                     GIDFFNLPEEEPDSPLGSSTGAGVIFGATGGVTEAALRTAYWMLFDKDLPKEAVEFKA
                     VRGMEGVRVATVDFEGLKVKAAVAHGLANANRVLDRLRGGEEFHWIEVMGCPGGCIGG
                     GGQPYAGAYAVPLDEAMLLCRSCALYDLDAARTLRLSHANPDIQGLYRDYLDKPMSHR
                     AHELLHTHYYARVPFGIRPQEAQL"
     gene            2581..3120
                     /gene="nuoE"
                     /locus_tag="PLQ54_05325"
     CDS             2581..3120
                     /gene="nuoE"
                     /locus_tag="PLQ54_05325"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOS92712.1"
                     /translation="MAYRREVLDTPDWWRELDEYINDQREKNRGHVQSALIPVLHHVQ
                     DRYGFIPDKAINHVAQALGVPTSYVCGVASFYSYFSLTPKGSFTVAVCLGTACYVQGS
                     QAVVDEFSRRLGIRPGETTADGLFTLVEARCLGACGQAPVVMVNDEVHAAVTPEQVPN
                     IIAHYTALAPREPASSNMA"
     gene            3137..>4050
                     /locus_tag="PLQ54_05330"
     CDS             3137..>4050
                     /locus_tag="PLQ54_05330"
                     /inference="COORDINATES: protein motif:HMM:NF028511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOS92713.1"
                     /translation="MRDVPLRTKLLVAIISVASVGGVLTLLAGGFLLNAQVIQEANRR
                     VELALKTARSALETRLAGTRTACSLLADELGAMNGGPELALPPEGLLEGLRDKAEFDY
                     LHLIDARGIIRATARGNATGQADSSPLVQVALKEGRAVDGFRIVPSSFLASESQELAA
                     KSHVQVIPTPRAKGDKQVDLSEAMVLEAASPVVVAPGRVIGVVRAGMVINRNSDMVDR
                     IRNTVFTVSTYKGKNFGTVTIFQDDVRIATNVTGPDGSRAIGTRVSAEVYDQVLRDGR
                     TWLGPAFVVGNWYVSAYEPIRDGQGRAVA"
BASE COUNT          684 a         1302 c         1381 g          683 t
ORIGIN      
        1 ctacttctgc accccatcac tctatttggg cggcggcgag taccagtcgg ccatgagcaa
       61 cgcctaccgg ggcctgttct tcctcgatgc ccccgtcggg ttcgttacgg acaagatgct
      121 tgcggagggg cgagcagacg gactcaaagt ggtggtgctc cccgcgtgcc ggtgggtcga
      181 gcgggagtcc ctggaccgcc tccgcgccct ggcggcccga ggcgtgaggc tggtcaccct
      241 cggcgacggg ccgaccttcg atgagtaccg ccacccgcac cctgccggca gcctcctgcc
      301 cgcagatgag gtgacgagcg tgcgccccgg cccgcccggc gacatccgcc ggcagatggc
      361 gcccatcctc gaccgcgcgc aggtccgccg ccccatccga tgcttccgag cggacggcga
      421 gacgcccgag gccgttgagt gccggagtgt ggtccacggg gacggtgtgc tctgcagcct
      481 gctgaacctc ggcaaagctc cggtgcggat tcgcctcgag caccatggcg agaccgtgcg
      541 ttcgtggaag gacctgatca gcgaccggga gtccgattcc gggtggatgg agttgcggcc
      601 cagcgacccg cgactgctgt ggatcgccgc cgatcggacc taggaagccg ccgcgcatcg
      661 gggacaggtg cgcggcgtgg tggacaggcg aatgcctttg tgatatcttt cacaaggcgc
      721 gaggcgcaac cacgcatcac ccgatctgcc actcctcgtc tgcctgaacc gccgatcttc
      781 cgcactctag tgcatgggtg ggaacactgt gggaaatgta gaactaacga ttaatggcgt
      841 cagggtagag gctgacagcg gctcgaccat catggacgcg gccgacaagg cggggatcca
      901 catcccacgg ctgtgccatc acccccggct gtccatgcag ggggcatgcc gcgtctgcat
      961 cgtcgaggtg gagggggttc ggaacaccgt cgcttcttgc tgttacccgg tccgggacgg
     1021 catggtggtc cgcacccaca cccccgagat tcagcggctg cgccgcgaca tcgtcgagct
     1081 gctgctggac aaccatccct cggactgtaa cacgtgcgag cggaacggca actgcgagct
     1141 gcagcgcctg gcgtcggcca tgggcatccg cgagcggctg ttcgcgggcg agcgcaagcg
     1201 gtatgagaag gacctctcca gcccgtcggt gatccgggac cccgagaagt gcatcctctg
     1261 tggccggtgt atccgcgtgt gcggcgagat ccaggaggac gcggccctca gccaggtcta
     1321 ccgcggcttc gacacggtgg tcatgcccgc gcacaacgcg gggttcatgg agaccgtgtg
     1381 tgtctcgtgc gggcagtgca tcaacgtgtg cccgacggcg gcgttcctcg aaaaggacgc
     1441 cacggcaccc ttcatgaagg ccatcgccga cccagacacc atcgtggtga gccagatggc
     1501 tccatcggtg cgagccgcca tcggcgagtg cttcgggctc cagccgggca gagcgtggga
     1561 gggacagtgc ttcgccgccc agcgcgctct gggtatcgac tacgtgttcg acacccagtt
     1621 cagtgccgat ctcaccatca tggaagaggc ggcggagttc gtggcgaagg tgaaggcggg
     1681 tggcccgctt cccaacatca cgtcctgctg tcccacctgg gtgaagtacg ccgagcactt
     1741 ccaccccgac atgctcaagt acctctcgtc ggccaagtcg cccatgagca tgcagggagc
     1801 cctggtgaag acctactggg ccgagaagat gggcgtggac cccgaccgga tcttctccgt
     1861 agccatcatg tgctgcacgt gcaagaagtt cgaggccacc cgccccgaac tgcatgtgga
     1921 tggccgagcc gcggtggacg cggtactcac cacacgcgag ttcgggtggc tgatcaagca
     1981 catgggtatc gacttcttca acttgcccga ggaggagccg gacagtccgt tgggcagctc
     2041 caccggagcg ggcgtcatct ttggggccac cggtggcgtc accgaggccg cgctgcgcac
     2101 ggcctactgg atgctcttcg acaaggacct gcccaaagag gccgtcgagt tcaaagcggt
     2161 gcggggcatg gaaggcgtcc gcgtggccac ggtggacttc gagggcctga aggtcaaggc
     2221 agcggtggcg cacggtctcg ccaacgcgaa ccgggttctc gaccgtctcc ggggcgggga
     2281 ggagttccac tggatcgagg tcatggggtg tccgggtggg tgtatcggcg gtggcgggca
     2341 gccgtacgcg ggcgcgtacg cggtgccgct ggatgaggcc atgctcctgt gccgttcttg
     2401 cgccctctat gacctggacg cggcccggac gcttcgcctg tcccatgcca acccggacat
     2461 ccagggcctg taccgcgact atctggacaa accgatgagt caccgagcgc acgaactgtt
     2521 gcacacccat tactatgcac gtgtgccgtt cggcatccgt ccccaggagg cacagctctg
     2581 atggcgtacc gacgcgaggt tctggacaca cccgactggt ggcgagagct tgacgagtac
     2641 atcaacgacc agcgcgagaa gaaccgaggc cacgtacaga gcgccctgat cccggttctg
     2701 caccacgtgc aggaccggta tgggttcatc cccgacaagg cgatcaacca cgtggcccag
     2761 gcgttgggtg tacccacctc atacgtgtgt ggggtggcca gcttctattc ctacttctcg
     2821 ctgacgccca aggggtcatt caccgtcgcc gtctgtctgg gtaccgcttg ctacgttcag
     2881 ggcagccagg cggtggtgga tgagttcagc cgccgtctgg ggatccggcc aggtgagacc
     2941 accgcggacg gcctcttcac cctggtcgag gcccgttgtc tgggcgcgtg tggccaagcg
     3001 ccggttgtga tggtgaacga tgaggtccat gccgccgtca cgcccgagca ggtgccgaac
     3061 atcatcgcgc actacacggc tctcgcccca cgggagcccg cgtcttcgaa catggcctga
     3121 gggagccccg gaccgcatgc gcgacgtgcc cctgcgtacc aagttgctgg tggcgatcat
     3181 ctcggtcgcc tcggtgggcg gagtcctcac gctccttgcc ggagggttcc tgctcaacgc
     3241 gcaggtgatt caggaggcca accggcgagt cgaactggcg ctcaagaccg cgcggtcggc
     3301 cctcgagaca cgcctggccg ggacgcgcac ggcgtgctcc ctgctcgccg acgagctggg
     3361 ggccatgaac ggaggcccgg agttggcgtt gccacccgag ggcctgctcg aagggctgcg
     3421 ggacaaggcg gagttcgact acctgcatct gatcgatgct cgcggcatca tacgggccac
     3481 cgcacgagga aacgcaacgg ggcaagcgga cagcagtccg ctggtccagg tcgcactgaa
     3541 ggaaggccgt gccgtcgacg ggttccggat tgtgccgtcc tcgttcctgg cttccgagag
     3601 tcaggagctg gcagcgaagt cgcacgtgca ggtgatccca acgccccggg ccaagggcga
     3661 caagcaggtc gacttgagcg aagccatggt tctcgaggcc gcctcgccag ttgtggttgc
     3721 tccgggccgt gtcatcggcg tggtgcgtgc gggcatggtg atcaaccgca actcggacat
     3781 ggtggaccgg attcggaaca ccgtgttcac cgtctcgacc tacaagggca agaacttcgg
     3841 cacggtgacc atcttccagg atgacgtccg gatcgccacc aatgtgaccg ggcccgacgg
     3901 gagccgtgcc atcgggacgc gggtttcggc ggaggtgtac gatcaggtcc tgcgcgacgg
     3961 gcggacgtgg ttggggccgg cattcgtggt gggcaactgg tacgtgagcg cctacgagcc
     4021 catccgcgac ggccagggcc gcgccgtggc
//
LOCUS       DAOSPL010000214         4047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22298_length_4047_cov_2.700150, whole genome shotgun sequence.
ACCESSION   DAOSPL010000214 DAOSPL010000000
VERSION     DAOSPL010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22298_length_4047_cov_2.700150"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PLQ54_05335"
     CDS             complement(<1..462)
                     /locus_tag="PLQ54_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92714.1"
                     /translation="MPRLSPKENLLRAIRYDDPEWVPRCNEPTMVGIAYEGNFRVACW
                     TDAWGTHWETTRDDMVPFPKGHPLPSLDALAEFAFPDPDVMFVLSEDAKSCLARAEKE
                     DQLIFGQQTYLLFERAWALMGMEAFMEAFYTHPEEMHELLGHITDYNIRIFE"
     gene            complement(552..1394)
                     /locus_tag="PLQ54_05340"
     CDS             complement(552..1394)
                     /locus_tag="PLQ54_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92715.1"
                     /translation="MARGKVRRPNEPGYGDYLRAAFNYRVPVRGLGGVPINWLFLGAV
                     GVASIVAWPVLLVGAGAEIGFLSALANSDRFQRSVRARMLQTRDEALLDQQDVAASGL
                     SPGAQARYAAFRQRCSEIVEIARKLQPADGSELQTYADHLAELRSVYVRMITLLGVFS
                     RYSVDWNKTDPEPAMRAVEKELEREDLSDSMRRSKEATLGVLRRRAESRKAISERAHV
                     LHSEIGRLEQEVALLRDQALLTRDPSVLTQSMDSAAGILEQHNAWLQENAAVFERLEQ
                     EEMA"
     gene            complement(1381..2304)
                     /locus_tag="PLQ54_05345"
     CDS             complement(1381..2304)
                     /locus_tag="PLQ54_05345"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006519908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS92716.1"
                     /translation="MAAPPTPDPPCLEIDHVSKEFRRPGQAPFVAVRDLCATVQDSAG
                     SGEFITIVGPSGCGKSTLLQLVAGFDTHVPPTTGEIRFHGEPVTGPSADRGMLFQHYG
                     CYPHLTVLDNIAFGLRLHARELGLSRRVIRDIALDWMRKVRLSGDDATKYPHELSGGM
                     RQRVALARCLALKPKCILMDEPFSALDEPTRFEMQDLIVDLWSDLRATILLVSHSVAE
                     AVYLGDRVWVMSPAPGTIVAEYRDVPLPSPDVPAMAEQSKPEFAAAVTEISGEFLRIL
                     RTPREELQAVVADGTGRNRPVEGRAVSSGAG"
     gene            complement(2308..3291)
                     /locus_tag="PLQ54_05350"
     CDS             complement(2308..3291)
                     /locus_tag="PLQ54_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOS92717.1"
                     /translation="MRRRGWLGLLVRPPQDPGPDDSGGAGEERSDAVASGPRPARAGS
                     GGRPRGKARPGPRFFRVREPLAPQWRLLLSLLPFVAFAAWWVWATAGPSPEVRRISPY
                     ALPSPGDIGEFLPSLWYDSALMRNILVSLVRVGAGFALAAAIALPLGVAMGSFTRIGA
                     TFSLVETVLGYLPIAAIVPLTMAWWHSGEKQKVGFLALATFSYLLPLIVRHVRKVDHQ
                     YLLAAYAQGATPWQVVFRVLVPIALPDMLNSLRLGLGVGWTYIVLCEVIKEGEGMGGV
                     GNLILVFQRLGKMEGVYPTVVAIMLVGALLDRACAKLWAWFFPYRIGLEGE"
     gene            complement(3348..>4047)
                     /locus_tag="PLQ54_05355"
     CDS             complement(3348..>4047)
                     /locus_tag="PLQ54_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOS92718.1"
                     /translation="EDVRGMLQDAHSTNFAENKQFFLNAANPTNFERTWNRITFVYQQ
                     LKKLDKAIAFDQVMDFSVIKALQDENKFASQQDTYTSKFAPSDWQRVAEAPLIRQVIR
                     IHFYPNSANLFEPARDEYGHIIQGQLYDPSVNDTLERVAQLAGQFDRAMIVISGHTDD
                     SYRDQVPYELVKKLSLDRAEAVRKALIDKHKFDPGKFKAEGRAWDVPADPNDPHNHAL
                     NRRVEIGVYPPEAE"
BASE COUNT          701 a         1372 c         1281 g          693 t
ORIGIN      
        1 ttcgaagatg cggatgttgt agtccgtgat gtgcccgagc agctcgtgca tctcctccgg
       61 atgcgtgtag aaggcctcca tgaaggcctc catgcccatc agcgcccatg cccgctcgaa
      121 cagcagatag gtctgctggc cgaagatgag ctggtcctct ttctctgccc gggcaaggca
      181 gctcttcgcg tcctcagaca gcacgaacat gacgtccggg tcggggaagg cgaactcggc
      241 cagcgcgtcg aggctgggta gggggtggcc cttcggaaag ggcaccatat cgtctcgtgt
      301 ggtctcccaa tgcgtgcccc acgcgtccgt ccaacaggcc accctgaagt tgccctcata
      361 tgcgatgcca accatagtcg gttcattgca gcgcggtacc cactcagggt catcataccg
      421 gatggcgcgg agcaggttct ctttgggcga gagccggggc atgcggcagg acctcctcga
      481 tgggcggcga cagggccccg cgtccggtgc aggagggcga gggcggcggc ggatgctcca
      541 cggtgcccga gtcaggccat ctcctcctgc tcgaggcgct cgaacacggc ggcgttctcc
      601 tgaagccacg cgttgtgctg ctcgaggatg cctgccgccg aatccatgct ctgcgtgagg
      661 accgacgggt ctcgagtcag cagagcctgg tcacgcagga gcgcgacctc ttgttcgagg
      721 cggccgatct ccgagtgcag cacgtgagcc cgttcgctga tggcctttcg gctctcggcg
      781 cggcgtctca ggacccccag cgtcgcctcc ttggaccggc gcatcgagtc ggacaagtcc
      841 tcgcgctcga gctccttctc caccgccctc atcgccggct cggggtcggt cttgttccag
      901 tcgaccgagt agcgagagaa caccccaagc agcgtaatca ttcgcacata gacagaacgc
      961 agctcggcca ggtggtcggc ataggtctgc agctccgaac cgtccgcggg ttgtagcttc
     1021 cgggcgatct cgacgatctc ggagcacctc tgcctgaagg ccgcgtagcg cgcctgggca
     1081 ccgggtgaca gcccgctagc tgcgacgtcc tgctgatcca gcagggcctc gtcgcgagtc
     1141 tgaagcatcc gtgcgcgcac cgaacgctgg aaacggtcgc tgttggccag agcactcagg
     1201 aagccgatct ccgcgcccgc cccgactagc agaacgggcc acgcgacgat gctggcaacg
     1261 cctaccgcgc ccaggaacag ccagttgatc gggacgccgc cgagcccgcg gaccggcacg
     1321 cggtagttga acgcggccct caggtaatcg ccgtatcccg gctcgtttgg cctccgaacc
     1381 ttaccccgcg ccactgctca cggccctccc ctcgaccggc cgattccgtc cggtgccatc
     1441 ggccaccacg gcctgcagct cctcgcgggg cgtcctgagg atacgtagga actcgccgct
     1501 gatctccgtg accgccgcgg caaactcggg ctttgactgc tcggccatgg ccggaacgtc
     1561 cgggctcggg agcggcacgt ctctgtactc agccacgatc gtccccggcg cggggctcat
     1621 tacccataca cggtcgccga ggtacaccgc ctcggccaca ctgtggctga ccaggaggat
     1681 cgtggccctc aggtccgacc acagatccac aatcagatcc tgcatctcaa accgcgtcgg
     1741 ttcatccaac gcgctgaaag gctcatccat caaaatgcac ttgggcttga gggccaggca
     1801 acgggccagg gcaactcgct gtcgcatccc gcccgagagt tcgtgcgggt atttcgtcgc
     1861 gtcatccccc gacaatcgca ccttgcgcat ccagtcgagc gcgatgtcgc ggatcacccg
     1921 ccgtgagaga ccgagctcgc gagcgtggag cctcaacccg aacgcgatgt tgtcgagcac
     1981 agtgaggtgc gggtaacacc cgtagtgttg gaacagcatg ccgcgatctg cgctcggccc
     2041 cgtgaccggc tcaccgtgga accgaatctc gccggtcgtg ggggggacgt gggtgtcaaa
     2101 ccccgccaca agctgcagta gcgtcgactt cccacatccg ctcggaccga caatggtgat
     2161 gaactcgcct gagcccgcag agtcctgcac cgtggcgcac agatcccgga ccgccacgaa
     2221 cggcgcctgc cccggccggc gaaactcctt gctgacgtgg tcgatctcga gacaaggcgg
     2281 atccggggtc gggggggcgg ccatggctca ctccccctcc agcccaatac ggtaggggaa
     2341 gaaccaggcc caaagcttgg cgcacgcgcg gtcgagcagt gcgcccacca gcataatcgc
     2401 cacaacggtc gggtagacgc cctccatctt ccccaggcgc tggaacacca ggatcaggtt
     2461 gcccaccccg cccatgccct ctccctcttt gatgacctca cacagaacga tgtaggtcca
     2521 tcccacaccg aggcccagcc gaagcgagtt cagcatatcc ggcagcgcga tcggcaccag
     2581 cacgcggaac accacctgcc atggggtcgc gccctgggcg tacgccgcca gaaggtactg
     2641 gtggtccacc ttccgcacat gccggacaat gagcggcagg aggtaggaga acgtagccag
     2701 cgccaggaag ccgactttct gcttctcgcc actatgccac cacgccatgg tgagtgggac
     2761 gatggcggcg atagggagat atccgagcac cgtctccaca aggctgaacg tggccccaat
     2821 gcgggtgaag ctccccatgg ccacgccgag gggcagcgcg atggctgctg cgagagcgaa
     2881 cccggcgccc acacggacga ggctcaccag gatgttgcgc atcaacgcgc tgtcatacca
     2941 caggctgggg aggaattcgc caatatcgcc cgggctgggc agagcatagg gtgagatgcg
     3001 gcgcacctcc ggcgatgggc ctgccgtggc ccacacccac catgccgcga atgccacgaa
     3061 gggcagcagc gacagcagga gacgccactg cggtgccagt ggttcgcgta cgcggaagaa
     3121 acgcggcccc ggccgggctt tcccgcgtgg cctcccgccc gaacctgccc gggccggccg
     3181 tggcccggat gccacggcgt cgctccgctc ctccccggcg cctcccgagt catcgggccc
     3241 cgggtcctgg ggcgggcgga ccagtaaccc cagccaaccg cggcggcgca tccgctccgg
     3301 ctcccccgtc tccgcactca cggctgtcgg gccgtcccca tgctggacta ctcggcctcc
     3361 ggcgggtaca caccgatttc gacccgccga ttcagtgcat ggttgtgcgg gtcattgggg
     3421 tccgccggca catcccacgc cctaccctcg gctttgaact tgcctgggtc gaacttgtgc
     3481 ttgtcgataa gtgccttgcg cacggcttcc gcgcggtcga gcgagagttt cttcaccagt
     3541 tcgtaaggta cctgatcgcg gtatgagtcg tccgtgtggc ccgatatcac gatcatcgcc
     3601 cggtcaaact ggccggcaag ctgcgccacg cgttcgagcg tgtcgttgac cgacgggtcg
     3661 tagagctggc cctggatgat gtggccatac tcgtcccggg ccggttcgaa caggttcgcg
     3721 gagttggggt agaagtgaat gcggatgacc tgccggatca gcggagcctc cgcgacccgt
     3781 tgccagtccg agggagcgaa cttcgacgtg tacgtgtcct gctgggacgc gaacttgttc
     3841 tcgtcctgca gtgccttgat gaccgagaag tccatcacct ggtcaaaggc tatggccttg
     3901 tcgagcttct tgagctgctg gtagacgaag gtgatgcggt tccaggtgcg ctcgaagttg
     3961 gtcgggttcg ccgcgttcag gaagaactgc ttgttctcgg cgaagttggt cgagtgggcg
     4021 tcttggagca tgcccctgac gtcttcc
//
LOCUS       DAOSPL010000215         4042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22332_length_4042_cov_3.554301, whole genome shotgun sequence.
ACCESSION   DAOSPL010000215 DAOSPL010000000
VERSION     DAOSPL010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22332_length_4042_cov_3.554301"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..2868)
                     /locus_tag="PLQ54_05360"
     CDS             complement(67..2868)
                     /locus_tag="PLQ54_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOS92719.1"
                     /translation="MRHSFHDRDEVSLMLTHLCILIATGAAPAAPSLVTNGDFEKDLA
                     GWSLAHTWYEKPAGGGLSEAIVVDGEGRDGGKALRITGGGKRGLAMQAPVAYPGRYRV
                     TGWLKCEGLEAGQAGVLAEWMDGKGTWLRGDWAAAVTGTRDWQAFDTTVEAPATTRSV
                     HIDLITTEPNDGTVWFDDITFERIASNLLKPNPPVIAAETPEGAEGCLKVTWDPASLE
                     DGVIRLVLYCVQRGDDGEQALPRGVADAESGEATIWSLANGEYYDVSAVAVNGDDVVS
                     ERGEAVRTRVSDRQPPRAGFVEARWLGRSRVSLSWSPHPLDFDLEAIRIVAPGAAEGE
                     LVALESLDAKQIAREARPFYCTAPWAEVDVELPAGQTRVGVQTEDAAGNASPVAWVEP
                     LPVRPADAGPVPCALWTEPPTAQLARSAEPPAVPSASFELELMGGQAKGFQVAIRPQA
                     DLHDVRVVFEPLAREGGGTIGPRWIAAHFVAYCNLERNSIATPADELVWKAPGDFPDE
                     LSDAPAMDLPAGQTQPVYIRVTAPSGSEPGVYSGRGYVDCAEGRSAFDIRVQIADASL
                     PARPRLKFVYWFGWGEPCKQFGVEQSSEDGWRVLARLGQLMKAHHQNTAVVSYGLVRS
                     WRMPDGSLAHDFADFDRFVRTMQEQGVDELFCIGHMGSRTTGEWECPTMSSHGYTITR
                     ADTGASEQIDVLELLPALQAHIEDIGLLDRFCVHVADEPIPQNLESYKQLSARVHAAA
                     PKLRRIDAIHVPDLQGSLEIWVPQLNYFEQWLDPYRAAQAAGNELWFYIAWVPQGKYP
                     NRMIDSAAIKPRVLHWLNALYDTTGYLHWALNWWDISLMSLNSPGDQYITWPSERFVA
                     NSSLRYEAEREGLEDCELMFMLRDALEKTLGSREQAQAEMEAMGRKAVRSIQDYTRDW
                     RELEATRQEILRRLEDSRG"
     gene            complement(3166..>4042)
                     /locus_tag="PLQ54_05365"
     CDS             complement(3166..>4042)
                     /locus_tag="PLQ54_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOS92720.1"
                     /translation="EDKDEFLRRFDDVVRSAEKHGIGLIPSLFWHTATVPDLVGEPVG
                     EWGNLQSKTHAYMREYVRDIVARYVDSPAIWGWEFANEFNLGANLPNAAEHRPPVWPD
                     LGTAASRSERDEWTYETIRTAFAAFADEVRKYDAYRVISTGDSLPRESGWHNWAKGTW
                     TADSPDQFALLLGADNPDPVDIISIHAYAGDAQRLRDARFVAAALCKPLFVGEFGAPG
                     PRSDSEAAFSELLATIEQAQVPLAAAWVYDFGNQDDFCITGGNERAYQLEAISAANER
                     IRGQLATEEAERGQP"
BASE COUNT          650 a         1413 c         1321 g          658 t
ORIGIN      
        1 ctttcatgat gcacggcgga cggaccgcct cctgtcgttc ttctggcccg ccatctcggc
       61 gccgcttcag cctctcgagt cctccaggcg ccgcaggatc tcctgccgcg tcgcctcgag
      121 ttcgcgccag tcccgggtgt agtcctggat gcttcgaacc gccttccggc ccatggcttc
      181 catctccgcc tgagcctgtt cgcgcgatcc gagcgtcttc tcgagcgcat cgcgcaacat
      241 gaacatgagc tcgcagtcct cgagcccctc ccgctcagcc tcatagcgca gcgagctgtt
      301 cgcgacgaac cgctcgctcg gccaggtgat gtactgatcg cccggcgagt tcaggctcat
      361 gagcgagatg tcccaccagt tgagcgccca gtgcaggtag cccgtcgtgt cgtacagcgc
      421 attcaaccag tgcaacacac ggggctttat ggcggcgctg tcgatcatcc ggttcgggta
      481 cttcccctgc gggacccagg caatgtagaa ccacagctca ttccccgctg cctgggcggc
      541 tcgatacggg tccaaccact gctcgaagta gttgagctgg ggcacccaga tctcgagcga
      601 gccctgcaga tcgggcacgt ggatggcatc gatgcgccgg agcttggggg cggccgcgtg
      661 cacccgtgcg gagagctgct tgtagctctc gaggttctgc gggattggct catcggccac
      721 gtggacgcag aagcgatcga ggagacctat gtcctcgatg tgagcttgca gtgcgggcag
      781 cagttcgagt acatcgatct gctccgacgc tcccgtgtcg gcccgcgtga tcgtgtagcc
      841 gtgcgaggac atggtgggac actcccactc gccggtggtc cgcgagccca tgtggccgat
      901 gcagaagagc tcgtcgactc cctgttcctg catggtccgg acgaagcggt cgaagtccgc
      961 gaagtcgtgg gcgagcgagc catcgggcat gcgccaggac cgcacgaggc cgtacgagac
     1021 gaccgccgtg ttctggtggt gggccttcat gagctgaccg agacgggcca gcacccgcca
     1081 gccgtcctcg gagctctgct ccacgccgaa ctgcttgcac ggctcgcccc agccgaacca
     1141 gtacacgaac ttcagtctgg gccgggcagg cagactggcg tcggcgatct ggacgcgaat
     1201 gtcgaaggcc gaacgcccct cggcgcagtc cacatacccc cggccgctgt agacacccgg
     1261 ttcggacccg gaaggggctg tcacacggat gtacaccggc tgcgtttgcc ccgcgggcag
     1321 gtccatggcc ggcgcgtcgg acaactcgtc ggggaagtcg ccgggtgcct tccagaccag
     1381 ctcatcggcc ggcgtggcga tggagttgcg ctcgaggttg cagtaggcca caaagtgggc
     1441 ggcgatccac cgcgggccaa tggtaccccc gccctcgcgc gccagcggct cgaagaccac
     1501 gcggacatcg tgtaggtcgg cctgaggccg aatggcgacc tggaagccct tcgcctgacc
     1561 acccatgagc tcgagctcga acgacgcact gggtacagcc ggcggctcgg ccgaccgtgc
     1621 gagctgggcc gtcggcggct ccgtccaaag agcacagggc accggaccgg catcggcggg
     1681 ccggaccggc aggggctcaa cccaggccac cgggctcgcg ttaccggcag cgtcctcggt
     1741 ctgcacaccc acacgcgttt ggcctgcggg aagttccaca tcgacctcgg cccacggcgc
     1801 ggtgcagtag aacggtcgag cctcccgcgc gatctgcttc gcatccagcg actcgagagc
     1861 tacgagctca ccttcggccg cgccgggcgc gacgatgcgg atggcctcga gatcgaagtc
     1921 gagtggatgc ggcgaccagg agaggctcac gcgactgcgc ccgagccagc gggcttcgac
     1981 gaacccggcg cgcgggggct gacgatcgga cacacgggtg cggacggcct cgccgcgctc
     2041 cgagaccacg tcgtcgccgt tcacggccac ggcggagaca tcgtagtact cgccgttcgc
     2101 aagcgaccaa atcgtcgcct caccggactc ggcatcggca acaccccggg ggagagcttg
     2161 ttcgccgtca tcgccgcgct gtacacagta gagaaccagc cgaatcacgc catcctccaa
     2221 tgaggcggga tcccaggtga ccttcaggca tccctcggcg ccctccggtg tctcggccgc
     2281 gatgaccggc gggttgggct tgaggaggtt tgacgcgata cgctcgaagg tgatgtcgtc
     2341 gaaccacacg gtaccgtcgt tgggctccgt cgtgatcagg tcgatgtgca ccgaccgcgt
     2401 cgtggccggc gcctcaacgg tggtgtcgaa cgcctgccag tcccgggtcc cggtcactgc
     2461 ggccgcccag tcgcctcgga gccacgtgcc cttgccatcc atccactcgg cgaggactcc
     2521 ggcctgaccg gcttcgagtc cctcgcactt caaccaaccg gtcacgcggt agcggcccgg
     2581 gtaggccaca ggcgcctgca tggccaggcc gcgtttgccc cctccggtga tcctcagcgc
     2641 cttaccccca tcgcgcccct cgccgtccac tacaatcgcc tcactgaggc cccccccggc
     2701 cggcttctca taccacgtgt gagccaggct ccaccctgcc aggtccttct cgaagtcgcc
     2761 gttcgtcacc agcgatggcg ccgccggggc ggccccggtg gcgatgagga tgcacaggtg
     2821 cgtgagcatg agagacacct cgtcgcgatc gtggaatgaa tgacgcatcg accttcgccg
     2881 cgcggcactg aaaccctcgg cgaccaacac cgccggggag gaggactgcc gtgggaactc
     2941 gttgggcgcg ctcgcggcca agcagcggaa gggcgccgtg tgggtaacgc gcgggccgtg
     3001 cggccgggtg cccctggggc tggcgacccg ggggacctcg aacgccacgg cggcatcggg
     3061 ccgggcccct ttcccctctg tcagcgtctg ccccgacggc ctgtcctgca acgggccgct
     3121 gggaccgcga agcctactgg cacgctggca gcgcagcccg cactgtcatg gctgcccccg
     3181 ctcggcctcc tcggtggcaa gctgcccgcg tatgcgctcg ttggcggccg agatagcctc
     3241 aagctggtag gcccgctcgt tgccgccggt gatgcagaag tcatcctgat tcccgaagtc
     3301 gtacacccat gccgccgcca ggggcacctg ggcctgctcg atggtcgcga gcagctcgga
     3361 gaacgcagcc tcgctgtcgg atcgaggacc cggtgcaccg aactcgccaa cgaacagcgg
     3421 cttgcagagc gcagcggcta cgaaacgcgc gtcccgaaga cgttgggcgt ccccggcgta
     3481 ggcatggatg ctgatgatgt cgacaggatc ggggttatcg gcgccgagca agagcgcgaa
     3541 ctggtccggc gagtcggccg tccacgtgcc cttggcccag ttgtgccagc ccgactcgcg
     3601 aggcagggag tcaccggtgg atatcacgcg gtacgcgtcg tacttccgta cctcgtcggc
     3661 gaacgcggcg aaggccgtgc ggatggtctc atacgtccac tcgtccctct cactccggct
     3721 tgcggcagtc ccaaggtcgg gccacaccgg gggccggtgc tcggccgcgt tcggcaagtt
     3781 ggcgcccagg ttgaactcgt tcgcgaactc ccacccccag atggccggcg agtccacata
     3841 gcgtgccacg atatcacgca cgtactcacg catgtaggca tgggtcttgc tctgcaggtt
     3901 cccccactcg ccgacgggct cacccacaag gtcgggcacc gtggccgtat gccagaagag
     3961 actcgggatg agcccgatgc cgtgcttctc ggccgaccgg acgacatcgt cgaaccgccg
     4021 aaggaactcg tccttgtcct cc
//
LOCUS       DAOSPL010000216         4030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22435_length_4030_cov_3.220377, whole genome shotgun sequence.
ACCESSION   DAOSPL010000216 DAOSPL010000000
VERSION     DAOSPL010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22435_length_4030_cov_3.220377"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="PLQ54_05370"
     CDS             <1..1181
                     /locus_tag="PLQ54_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOS92721.1"
                     /translation="TVLAEGLPSRADLRPDYASLGLKPKNQNPRGCCSLFAMVGVLEF
                     ELAKATGKPVSLSEEYMNWASHQTNGRGSDGSFFSDALRGAECFGICAETLMPYQREF
                     RPDAEPSVEAFRDAETRKGVSALWIKEWDVTTGMTNAMLAQIRESVATGHPVAIGMRW
                     PKTDTYLGDQLLTPPRPEDVFDGHSVVIVAYEDDAAQPGGGTFVFRNSFGPEWGEGGY
                     ARMSYAYAQAYGNDALGLRIGGGRTVPSNRVAADPIEVETLEVAQRRGCQSADPPPLL
                     WCPALWSGGGQVAYAAEVGARLDLRLIAPAAGTYRVWLFGTAGPSFGEIRMTLDGERA
                     GRDIDLYATELRPTGRIDLGVHTLEAGARALRLDVIGKDHRSSGYDFGLDCIELERVL
                     "
     gene            1184..3748
                     /locus_tag="PLQ54_05375"
     CDS             1184..3748
                     /locus_tag="PLQ54_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92722.1"
                     /translation="MGTPEELRAKAAGGTFCIVPYCHPDFAWTHHREWHEERYAVSIS
                     EALDLMGECPQFRFCIEPWIDQVQPFLERCPERADELRERLTTGQMGVKAFTLVSPRP
                     ATCPEEAFVRNMVLGRRRYREFAPEADLSVMACPDVCIGYSQMPQVVRLAGGRMYRGW
                     RSEAAFNALGIPRDFVWRGLDGTELITSRGIYGGLCWDPAIPEDFADCWEELTSRLFG
                     AELGDAMTNSSTPLWWVAQGMDDSRPLCAWPRDKRQPVVAFIDEWNRRESSHMRFATP
                     NEYGRMLAALDLPVWDGVIDQVDVAYNSGWHGARGLWRLRQRLDTAAVIAERACAMAR
                     LAGGQAAGPDRLAELWTEVVRVQSHALQWVFEKDWEWINTRARYALRELAEATHDAVC
                     ALAGAGRGFGEDRPLVLFNPLPYARDEWIEVPWVQPRQDAGGHQVIGPDGRAVELQLG
                     EQAGESWGDQVVEAPLIFRARVPALGCAIYRVADGPFEAAPPAPEGDVVDNGRLRLAL
                     SGRGLQSVEDSVTGTEWRAPRGSAIGDCRLFEMGPGVLGVGPITAEMSGEAGQGRWIS
                     TGPIRWRYRWETRFHGHRVRQDIVVDRDEGHVDFVTRVFCIGANGFFALCFDLPVRGV
                     MHVDIPFGVEPRDLSHEVYAMDMPPGMHNIERNREDQFWARSWASVSDGEKGVAVITL
                     DGDRYWTYEAGPGRLRHILFMALHDVDTSWEAWVPKDRMALGWHEFQHRVVFHQGDWR
                     AAGLCGESDRVRLPLQAVKPVGPPGDRKLGRPLEVSPKSARLSAFYEAPQGHVLRVYE
                     SAGESVTADITLPHGFAVARRTDFNLEPMEATCLLDGDRLSVPLRPWEIATVLLEG"
BASE COUNT          604 a         1254 c         1497 g          675 t
ORIGIN      
        1 ccaccgttct ggccgagggc ctgccttcgc gagctgatct tcggcccgac tacgcgtcgc
       61 ttggcctcaa gcccaagaac cagaatccgc gcgggtgctg ctcgctcttc gccatggtcg
      121 gtgtcctcga gttcgagctg gccaaggcca ccggtaagcc cgtctcgctg tctgaggagt
      181 atatgaactg ggcatcgcac cagaccaacg gacgcgggag tgatggttcg ttcttctcgg
      241 atgcgctccg cggcgccgag tgcttcggca tctgcgccga gacgctcatg ccctaccagc
      301 gtgagttccg ccccgatgcc gagccatcgg tcgaggcgtt tcgcgatgcc gagacgcgca
      361 agggcgtgtc ggctctgtgg atcaaggagt gggacgtcac gaccggcatg accaatgcca
      421 tgctggcgca gatccgcgag agcgtcgcga cgggccaccc ggtggcgatc ggcatgcggt
      481 ggccgaagac cgacacctac ctgggcgacc agttgctcac tccgccgaga cccgaggatg
      541 tgttcgacgg ccacagcgtg gtgatcgtgg cctatgagga tgacgcggcc cagccgggcg
      601 ggggcacatt cgtcttccgc aactccttcg gccccgagtg gggcgagggc gggtacgccc
      661 gcatgtcgta cgcctacgcc caggcctacg gcaacgacgc gctgggcctg cgcattggtg
      721 gaggacggac ggtgccgagc aaccgggttg cagccgatcc catcgaggtt gagaccctcg
      781 aggtcgccca acggcgtggg tgccagtccg ccgacccacc gccccttctg tggtgccctg
      841 cgctatggag tggcggcggg caggtcgcct atgcggcgga ggtgggagcc cggctggacc
      901 tgcgactgat tgctcccgcg gcgggtacct atcgagtttg gctcttcggg acggctgggc
      961 cgagcttcgg tgagatccgg atgacgctcg acggagagcg ggcgggtcgt gacatcgacc
     1021 tgtatgccac ggagctacgg cccaccggtc gcattgacct cggggtacat acgctggagg
     1081 cgggtgcgcg ggcactccgc ctggacgtga tcgggaagga ccatcggtcg tcggggtatg
     1141 acttcggcct ggactgcatc gagctggaga gggtcttgta gccatgggaa cgcctgagga
     1201 gcttcgagcc aaggcggccg gcggtacctt ttgcatcgtc ccctactgcc acccggactt
     1261 cgcgtggacc caccaccggg agtggcacga ggagcgctat gcggtcagca tcagcgaggc
     1321 cctggacctg atgggggaat gcccccagtt ccgtttctgc attgagccat ggatcgacca
     1381 ggtgcagccg ttcctcgagc gctgccccga acgcgcggat gagctgcggg agcggctgac
     1441 gacgggacag atgggcgtga aggcgttcac gctcgtgagc ccgcgtccgg cgacgtgccc
     1501 ggaagaggca ttcgtgcgga acatggtcct cggccgccgc aggtaccgtg agttcgctcc
     1561 ggaggccgac ctgtcagtga tggcgtgccc ggatgtgtgt atcggctaca gccagatgcc
     1621 ccaggtggtg cgtttggccg gcgggcggat gtaccggggg tggcggtcag aggcggcgtt
     1681 caacgccctg ggcattccgc gcgacttcgt ctggcgtgga ctcgacggca ccgagttgat
     1741 cacgtcgcgc ggcatctacg gcgggctgtg ctgggacccc gccatcccgg aggacttcgc
     1801 cgactgctgg gaggagctca cgagccgcct cttcggcgcc gagctgggtg acgccatgac
     1861 caactcgagc acgcccctgt ggtgggttgc ccaagggatg gatgattccc ggccgctgtg
     1921 tgcgtggccg cgcgacaagc ggcaacccgt ggtggcgttc atcgacgagt ggaatcggcg
     1981 cgagtcgagc cacatgaggt tcgccacgcc caacgagtac ggccgaatgc tcgcggcgct
     2041 cgacctcccg gtgtgggacg gcgtcattga ccaggtcgac gtggcctaca acagcggttg
     2101 gcacggtgcc cgcggcctct ggcgcttgag gcagcggctc gacacggcgg ctgtcatcgc
     2161 ggaacgtgcc tgcgccatgg cccggctggc aggaggccag gcggctggcc cggatcgcct
     2221 tgccgagctg tggaccgagg tggttcgcgt ccagtcgcat gcgctgcagt gggtcttcga
     2281 gaaggactgg gagtggatca acacccgggc ccgctacgcg ctcagggagc ttgccgaggc
     2341 gacccacgat gccgtctgcg cgctggccgg tgccgggcgc ggcttcggcg aggaccggcc
     2401 tctggtgctc ttcaacccac tcccgtatgc gcgcgacgag tggatcgagg tgccgtgggt
     2461 tcagccgagg caggacgctg gcggacacca ggtcatcggc ccggacggcc gggcggtcga
     2521 gctgcagctt ggtgagcagg cgggagagag ctggggcgac caggtggtgg aggcgccgct
     2581 gatctttcgc gcgcgcgtgc cggcactcgg gtgcgcgatc tatcgggtgg ccgatggtcc
     2641 cttcgaggcg gcgccaccgg cgcccgaagg tgatgtggtg gacaacggac gactgaggct
     2701 ggcgctgtcg gggcgcggcc ttcagtcggt cgaggactct gtgactggaa ccgagtggcg
     2761 ggcccctcga gggtcggcca tcggtgactg ccggctcttc gagatgggtc ccggcgtgct
     2821 gggcgtgggg cccatcacgg cggagatgtc cggcgaggcg ggccaggggc ggtggatcag
     2881 cacggggccg atccgatggc gctatcgttg ggagacgcgc ttccacggcc atcgggttcg
     2941 gcaggacatc gtggtcgacc gcgacgaggg gcatgtggac ttcgtcaccc gcgtgttctg
     3001 catcggcgcc aacgggttct tcgcgctctg cttcgacctg ccggtccgcg gggtcatgca
     3061 tgtcgacatc ccgttcggcg tggagccgcg ggacctgtcg cacgaggtct acgccatgga
     3121 catgccgccg ggcatgcaca acattgagcg gaaccgggag gaccagttct gggcgcgaag
     3181 ctgggcatcc gtgtcagacg gcgagaaggg tgtcgcggtc atcaccctcg acggcgatcg
     3241 ctactggacc tacgaggctg ggccgggccg gctgagacac atcttgttca tggccctgca
     3301 tgacgtggac acctcgtggg aggcatgggt gcccaaggac cgcatggcac tgggctggca
     3361 cgagttccag catcgtgtcg tcttccacca gggcgattgg agggccgccg gcctatgcgg
     3421 tgaatcggac cgcgtccgac tgccgctgca ggccgtgaag ccggtagggc ctccggggga
     3481 ccgcaagctc ggccgcccac tggaggtgtc gccgaaatcc gcgcggctat cggctttcta
     3541 tgaggcgccc cagggccatg ttctccgggt gtacgagtcg gcaggcgaga gcgtgactgc
     3601 tgacatcaca ctgccgcacg ggttcgccgt cgcgcggcgg acggacttca acctggagcc
     3661 gatggaggcg acttgccttc tggacggaga ccgcctgagt gtgccgctca ggccctggga
     3721 gatcgcgacg gtgttgctgg agggttagca cgggccggcg gctgggccga ctgcaaaagc
     3781 gcccgggccg agtggaagtg gcccgcgagg cggggagggc gccatccgat ccgttcgggc
     3841 ggaaggcgct acagtaccag gcacgggaca gggcgatgac ggagcaggag cagtccggag
     3901 tcagacccgc cgaggtcgaa gcgcacccgt ccgcgcgcgt cgtgggtggg gtcacgcctc
     3961 gatccgtcgt catcagcctg gtcgtggtgt tcctgtgcgt gttctggatc cgccaggccg
     4021 aactgctctc
//
LOCUS       DAOSPL010000217         4028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22448_length_4028_cov_3.088346, whole genome shotgun sequence.
ACCESSION   DAOSPL010000217 DAOSPL010000000
VERSION     DAOSPL010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22448_length_4028_cov_3.088346"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..1031
                     /locus_tag="PLQ54_05380"
     CDS             33..1031
                     /locus_tag="PLQ54_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92723.1"
                     /translation="MPPLPWIISGLATAALAFGAERLAPPFPPVPEAERLVGIAYTTW
                     NRSTQWTGVWGTPELGDYVSTDRAIIRQHGEWLADAGVDFIWIDWSNNLDYVPGVTQN
                     RPDFDTIEGATHAIFEEYAGMQRRPRISIFIGCPGAPEAVTDGRLTRKASQVYDEFVA
                     EPRFRPLVQDYLGKPLLVVYVNTPSPWQKGLPAWDDPRFTVRFMTGFITEQPSLRTDD
                     LVSRYGYWSWEDRGKQTYSVYDGRPEAMTIVACWRPDPGIPTPGRRGGETFRQEWARA
                     REIGPRFALVVSWNEWVLSEQPSAEVSKDLEPSVEHGRLYLDLLTQEIARFKGRAV"
     gene            complement(1042..>4028)
                     /locus_tag="PLQ54_05385"
     CDS             complement(1042..>4028)
                     /locus_tag="PLQ54_05385"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOS92724.1"
                     /translation="GPNEVIVRKAPSDKNDDYLYLSIDHTERRGNSAVSFDGQSWTQD
                     ALTIPGGNGEYMVRLHLLTEPMVVAATWRPGQAQPLEDTSGLLLYAGAIGADAQPGGL
                     RLRAGQCAQMEWEPDALDRLAQAVVRVQAVGKYSLSWLDRSGKSVGAVACSGPGELTG
                     AAERAECYSGVRITAADGPVTVREVSLSAERSIRPLPRRVNMRPDIALPRAIPHRRVP
                     VCVVSRTSIRLANSGLRCRFDVGERLRLGSLYNEHTAFEMLRDPAQAHLFLVEVDGKR
                     YAGNRDFRCARLEKRGRDGFAATLELDEPRLSATLTGRMDDEGLRLGLVLSNAGGRPV
                     DFKLAFPFVAGLAASERLADDYYFFPWGGGIIADTPAVIRRGYGNHEALFQMMDLFSP
                     ARGGGLYVRADDAEGWHKGLALRKHVLGHPEENHEANSTPTAREYTWARPFEPAEGFG
                     VAYEYLRRTRQPGDRFMPAEAVLAAHPGDWHTAMAAYAAWAHRVWRFRPFPSRLAPIN
                     NMIAAGWDGDFLFRDGQYRTDFIHANTDCIELMSWWDWSPLGPWRTPFDQLSKVLSPE
                     AIRDWEPYFVKDPVTGQTMWNNQPGDYDGYNERFGGLPAFRAAIETYRQRGALVTLYT
                     DPLRLDDGSKMGQRHGKEWTVIAPNGDEVRNYDVWTPCHDVPDYRKWVADTMERVMRE
                     TGADGIRLDEYGHAGWACFSAAHAHTFQERGVSQWLKEIADTTRQVREAMDRVDRTTV
                     LTIEHPGYDYLWQYIDGCLTYDLTVQSCPLRPVEVNLQRFYFPECKVFELDHQGVDPQ
                     DKRKFFSATGSFGRYYPPPMYAALRDNQSVYQTGKAEALVPTLVRYVYANRFSDDRKT
                     IVHLFNGSGHTVSGPLIELSLAGDQHVFDLLRGREVTVSGSGDRTAAVGDLARDDVMA
                     VIVLPRQLDVAVTGDVVTVRSTAAGPGTRIVVSDADGNELVPSATGSTARFSLAALRA
                     AGAPQCVKLLQGPELVDAAELR"
BASE COUNT          687 a         1406 c         1270 g          665 t
ORIGIN      
        1 ctgtcgaggc acagcaggag gtcggcccca ccatgccccc actcccctgg atcatatccg
       61 gcctcgccac ggccgcgctg gcattcggcg cggagaggct cgcgccgccc ttcccccccg
      121 ttcccgaggc cgaacgactc gtgggcatcg cctacacgac atggaaccga tccacccaat
      181 ggacgggtgt atgggggacg cccgagttgg gcgactacgt gtccaccgac cgcgccatca
      241 tccgccagca cggcgagtgg ctggccgacg ccggcgtgga cttcatctgg atcgactggt
      301 ccaacaacct cgactacgtc cccggagtga cccagaaccg gcccgacttc gataccatcg
      361 agggggccac acatgccatc ttcgaggaat acgcaggcat gcagcgccgc ccgaggatca
      421 gcatcttcat cgggtgcccg ggggcgccgg aggccgtcac cgacggtcgg ttgacgagga
      481 aggcgagcca ggtctacgac gagttcgtgg ccgagccacg gttccgtcct ctggtgcagg
      541 actacctagg caagcccctt ctggtggtct acgtgaacac accgtcgccc tggcagaagg
      601 ggctgcccgc atgggatgat ccgcggttca ccgtgcggtt catgaccggc ttcatcaccg
      661 aacagccgag cctgcgcacc gacgacctgg tcagccgcta cggctactgg tcgtgggagg
      721 atcgtggaaa gcagacgtac tctgtctacg acggccgccc cgaggccatg accatcgtgg
      781 cctgttggcg gcccgatccc ggcatcccca caccgggccg gcgtggcggt gagacgttcc
      841 ggcaggagtg ggctcgtgcc cgtgagatcg gcccacgatt cgccctcgtg gtctcgtgga
      901 acgagtgggt actcagtgag cagcccagcg cggaagtcag caaggacctc gaaccgtccg
      961 tcgaacatgg ccggctctat ctcgacctgc tgacacagga gatcgcgcgg ttcaaaggcc
     1021 gtgccgtctg acacatgcga cctagcggag ctccgccgca tcgaccagct cgggcccttg
     1081 cagcagcttc acgcattgcg gcgctccagc ggccctcagt gccgccaggc tgaagcgtgc
     1141 cgtcgatccg gtcgccgaag gcaccagctc gtttccgtcg gcatcggaca cgacgatgcg
     1201 agtccctggc ccggcagccg tcgaacgcac cgttacgaca tcaccggtca ccgctacgtc
     1261 gagctgccgc ggcaacacga taacggccat tacgtcgtcc cgggccaagt cacccaccgc
     1321 cgcagtccgg tcgcccgagc cggagacggt cacctcgcgg ccccgcagca ggtcaaaaac
     1381 gtgctggtcg cccgccaaac tcagctcgat cagtggcccc gagaccgtgt gccccgaccc
     1441 gttgaacagg tgcacgatgg tcttgcggtc gtccgagaaa cggttggcgt agacgtagcg
     1501 aaccaacgtc ggcaccagtg cctcggcctt gcccgtctgg tagacgctct ggttgtcgcg
     1561 tagggccgcg tacatcgggg gcgggtagta ccgcccgaat gagcccgtcg cgctgaagaa
     1621 cttgcgcttg tcctgcggat ccacgccctg gtggtcgagt tcgaagacct tgcactcggg
     1681 gaagtagaac cgttgcaggt tgacctccac gggccgaagc gggcacgact gcacggtcag
     1741 gtcataggtg agacagccat cgatgtactg ccagaggtag tcgtaccccg ggtgctcgat
     1801 ggtcagcacg gtcgtacgat caacccggtc catggcctcg cgcacttggc gcgtcgtgtc
     1861 ggcgatctcc ttcagccact ggctgacccc gcgctcctgg aacgtgtgcg cgtgggccgc
     1921 actgaaacac gcccaacccg catgcccgta ctcatcgaga cggatgccat ccgcaccggt
     1981 ctcgcgcatg acacgttcca tggtgtcggc gacccacttg cggtagtcgg gcacatcgtg
     2041 gcacggcgtc cagacatcgt agttgcggac ctcatcgccg ttgggtgcga tcaccgtcca
     2101 ctccttgcca tgccgctgcc ccatcttgct gccgtcatcc aaccggagcg ggtcggtgta
     2161 gagggtgacc agggcgcccc tctggcggta ggtctcgata gccgccctga acgccggtag
     2221 gccgccgaat cgctcattgt agccgtcgta gtcgcccggc tggttgttcc acatggtctg
     2281 accggtgacc ggatccttga cgaagtaggg ctcccagtcc ctgatcgcct ccgggctgag
     2341 caccttgctg agctggtcga atggggtgcg ccacggcccc agcggcgacc agtcccacca
     2401 cgacatcagc tcgatgcagt cggtattggc gtggatgaag tccgtccggt actggccatc
     2461 gcgaaacagg aagtcgccgt cccaccccgc cgcgatcatg ttgttgatcg gcgccaggcg
     2521 cgacggaaac gggcggaacc gccacacgcg gtgcgcccag gctgcatagg cagccatcgc
     2581 ggtgtgccag tcgccgggat gcgccgcgag caccgcctcg gcgggcatga accgatcgcc
     2641 gggctgccgc gttcgccgca ggtactcata ggcaacacca aacccctcag ccggctcgaa
     2701 cggtcgagcc cacgtgtact ccctggcagt tggggtgcta tttgcctcgt ggttctcttc
     2761 ggggtgcccg agcacgtgct tccggagtgc cagccccttg tgccagccct cagcatcgtc
     2821 ggcgcggacg tacagcccac cgccgcgcgc ggggctgaac aggtccatca tctggaacaa
     2881 cgcctcatgg tttccatagc cgcgccggat caccgctggg gtgtcagcga tgatcccgcc
     2941 gccccacggg aagaagtagt agtcatcggc gaggcgctcc gaggccgcca atcctgccac
     3001 aaaggggaac gcgagcttga aatcgaccgg cctccctccc gcgttgctca gcacgaggcc
     3061 cagtctcagc ccctcgtcat ccatacggcc cgttagtgtc gccgacagcc gcggctcgtc
     3121 gagctccagt gtggccgcga agccgtcgcg gcctcgcttc tccaggcgcg cgcaccggaa
     3181 atcccggtta cccgcgtacc gcttgccgtc gacctccacc aggaagagat gcgcctgcgc
     3241 cggatcgcga agcatctcaa aggcggtgtg ctcgttgtag agcgaaccca gcctcagccg
     3301 ttcacccaca tcgaaacggc acctaaggcc tgagttcgcc agccgaatgc tcgtccggct
     3361 caccacacag acgggcacac gacggtgagg aatggcacgc ggcagggcga tgtcggggcg
     3421 catgttgacg cgccgcggca gggggcggat cgagcgttcc gctgacagac tgacctcgcg
     3481 gacagtaacc ggcccgtcgg ccgcggtgat acgcaccccg ctgtagcact cagcccgctc
     3541 ggcggctccg gtaagctccc cggggccgct gcaggcgacg gcgccgaccg acttcccaga
     3601 gcgatcgagc caggacaggc tgtacttccc cacggcctgc acgcgaacga ccgcctgcgc
     3661 aagccgatcc agtgcatccg gctcccactc catctgagca cattggccgg cccgcaatcg
     3721 aagccctccg ggctgtgcat cggcgccgat cgcccccgca tagagcaaga ggcccgatgt
     3781 gtcctcgagt ggctgggcct ggccgggacg ccatgtcgcc gctacgacca tgggctccgt
     3841 cagcaagtgc agccggacca tgtactcccc gttgcctccg gggatcgtca gggcgtcctg
     3901 cgtccacgac tgcccgtcga aagacacagc gctgttgccg cgccgctctg tgtggtcgat
     3961 gctgaggtac aggtagtcat cgttcttgtc actcggcgct ttgcgtacga tgacctcgtt
     4021 ggggccca
//
LOCUS       DAOSPL010000218         4027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22460_length_4027_cov_3.256294, whole genome shotgun sequence.
ACCESSION   DAOSPL010000218 DAOSPL010000000
VERSION     DAOSPL010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22460_length_4027_cov_3.256294"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2855)
                     /locus_tag="PLQ54_05390"
     CDS             complement(<1..2855)
                     /locus_tag="PLQ54_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HOS92725.1"
                     /translation="MACYGPPRKQGLYDPANEHDACGIGFVCDIHGRKSNAVLRQGLE
                     VLLALCHRGASGAEPDTGDGAGVLIQLPHAHLQRVCSADGITLPAPGHYGVGMVFLPQ
                     DPALRAQMEGRFETIVAEEGLTFLGWRTVPTDNATVGQTARACEPVVRQCFIARGPDI
                     TDGQAFERKLFVARKRAENAIRHSGEEGALAFYFASLSSRTIVYKGMLTSHQVEEYYT
                     DLSDPAVETAICLVHSRFSTNTFPSWDRAHPYRLIIHNGEINTLRGNVNWMRARQAML
                     ESDLFGDDLKKVLPVIDPEGSDSAMFDNCLEFLTLGGYSLPQAVMMMIPEPWSKHEGM
                     SDERRAFYEYFSCLMEPWDGPASIGFTDGIRVGAVLDRNGLRPSRYYVTRDGLVVLAS
                     EVGVLDIPADQVLRKGRLQPGRMFLVDTEQGTIVGDDELKTSMAAARPYRQWLDENLV
                     ELADLPPASATAPVAGTEAPLLQRQHAFGYTYEDLRILMAPMARDAVEPLGSMGNDVP
                     LAVLSERPQLLFSYFKQLFAQVTNPPIDAIREEIITALETTVGSERNLLSPEPASCRM
                     LRLPTPIISDEDLARIRQLDAPGLRAATFPIHFDPAEGGQGLESAMAELCARVDQAIA
                     DGVTIVVLSDRGVSPSQAPIPSLLAVSGLHHHLVRNETRTRVGIVLESGEPREVHHFA
                     VLIGYGCGAINPYLACESVVGLVEAGLVTGVSREKAVANYLKAAVKGVVKVMSKMGIS
                     TVQSYRGAQIFEAVGLNQAVIERYFSGTASRIGGIGLPEIAREAALRHQAAFARCGQL
                     PPLDSGGHYQWRRDGEHHLFNPESIHALQYATRTGSYEAFGRYSRLINEQSRRLATLR
                     GLLEFRYAERPIPIDEVESVDSICKRFKTGAMSFGSISREAHECLAIAMNRIGGKSNT
                     GEGGEDPARFVPDDNGDSRCSAIKQVASARFGVTSNYL"
     gene            complement(3199..>4027)
                     /locus_tag="PLQ54_05395"
     CDS             complement(3199..>4027)
                     /locus_tag="PLQ54_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92726.1"
                     /translation="QAPAAEVAPAEPPAAQDEAAQLEAMLQELGAPPDMRALFTAMAQ
                     EDADPMMLMMLMGMAGGPGGGGDGDAIGFAMLMRALGSQGKQPTAVANGDKLLVIEDG
                     VVYRIDLNKMAVDGQVAYRPKKSAVAGLVKLLPLIAGAARPKAEQAACLSNAKQIGLG
                     IMMYAQDHDQTLPTEDWVEELMPYIKNEQLLRCPAAGDAMPAYGFNEKLLGLTLADIA
                     KPAETVLVFEKDPADGTNVGGPEQLAARHEGSVVIGFADGHVQVMPLDQVMRLRWDP"
BASE COUNT          678 a         1349 c         1304 g          696 t
ORIGIN      
        1 aggtagttgc tggtcacgcc gaagcgcgcc gaagccacct gtttgatggc gctgcagcgt
       61 gagtcgccgt tatcatcagg gacgaaacgc gcggggtcct ctcccccctc acccgtgttg
      121 ctcttgcccc cgatgcggtt catggcgatg gccaagcact cgtgggcctc gcggctgatg
      181 gagccaaatg acatggctcc cgtcttgaaa cgcttgcaga tggagtcgac cgactcgacc
      241 tcgtcgatgg ggatgggtct ctcggcgtaa cggaactcca gcaggcctcg cagcgtggcc
      301 aggcgccgtg actgctcgtt gatcagccgc gagtagcggc cgaaggcctc atagctgccg
      361 gtacgtgtcg cgtactgcag ggcgtgaatg gactcggggt tgaacaggtg gtgctcgcca
      421 tcgcgccgcc actggtagtg cccgcccgag tcgagcggcg gcagttggcc acaccgggcg
      481 aacgccgcct ggtggcgcag tgccgcctcg cgtgcgatct ccggcaggcc gatcccgccg
      541 atgcgggatg cggtgccgct gaagtagcgt tcaataacag cctgattcag gcccacggcc
      601 tcaaagatct gagcgccgcg atagctctgg accgtcgaga tgcccatctt ggacatgacc
      661 ttgacgacgc ccttgacggc cgctttcagg tagttggcga ctgccttctc gcggctcacg
      721 cccgtcacga gacccgcctc gaccagaccg acaacggact cacatgccaa gtaaggattg
      781 atcgccccgc aaccatatcc aatgagtacg gcgaagtgat gcacttcgcg aggctctccc
      841 gactcgagaa caatgccaac ccgcgtccgg gtctcattgc gcaccaggtg atggtgcagg
      901 cccgatacgg ccagcaacga ggggatgggc gcttggctcg ggctgactcc gcggtccgac
      961 aggaccacga tggtcacccc atccgcgatg gcttggtcca cccgggcgca gagctcggcc
     1021 atggccgact cgagtccctg cccaccctcc gccggatcga agtggatcgg gaaggtagcg
     1081 gctcgcaacc ccggagcgtc gagctggcga atccgcgcca ggtcctcgtc ggagatgatc
     1141 ggagtcggca accgcagcat ccgacagctg gcgggctcgg ggctcaagag attccgctcc
     1201 gagcccacag tggtctcgag ggcggtgatg atctcctcgc ggatcgcgtc gatcggcggg
     1261 ttcgtgacct gggcgaagag ctgcttgaag taggagaaca acagctgcgg ccgctccgac
     1321 agaacggcca acggcacatc gttgcccatg gaccccaagg gctccaccgc gtccctcgcc
     1381 atgggagcca tgaggatgcg caggtcctcg taggtgtacc cgaaggcgtg ctgccgctgg
     1441 agcaagggtg cctccgtgcc agccaccggc gccgtcgccg aggccggtgg gaggtcggcc
     1501 agctccacca ggttctcgtc cagccactgc cgatagggcc gggcggccgc catggacgtc
     1561 ttgagctcat catcgcccac gatggtgccc tgctccgtgt cgacgaggaa catgcggccc
     1621 ggctgcagtc ttcccttgcg cagcacctga tcagcgggga tgtccagcac tccgacctcg
     1681 gacgccagca cgacgagccc atcgcgagtc acgtagtagc gtgaaggccg caggccgttc
     1741 cggtcgagca cggcgcccac ccggatccca tcggtgaagc cgatggaggc cggcccgtcc
     1801 cagggctcca tgaggcatga gaagtactcg tagaacgccc gccgctcgtc actcatgccc
     1861 tcgtgcttgg accacggctc ggggatcatc atcatgaccg cctgcgggag gctgtagccg
     1921 ccgagcgtga ggaactcgag gcagttgtcg aacatggccg agtcgctgcc ctctgggtcg
     1981 atgacgggaa gcaccttctt gaggtcatcg ccaaacaggt cggactcgag catagcttgc
     2041 cgggcgcgca tccagttcac attaccgcgc agggtgttga tctcgccatt gtggatgatc
     2101 aggcggtatg gatgggctcg gtcccagctc gggaatgtgt tggtcgagaa tctcgagtgg
     2161 acgaggcaga tcgcggtctc gaccgcggga tcggaaagat cggtatagta ctcctccacc
     2221 tggtgagacg tgagcatgcc cttgtagaca atggtccggg acgacaggct ggcgaagtag
     2281 aaggccagcg cgccctcctc gcctgagtgc cgaatcgcgt tctccgctcg cttccgggcc
     2341 acgaacagct tgcgctcgaa ggcttggccg tcggtgatgt ccgggccgcg ggcgatgaag
     2401 cactgccgca ccacgggctc gcaggcccgt gccgtctgcc ccacggtggc gttgtccgtc
     2461 ggcacggttc gccagcccag gaacgtcagc ccctcctccg ccacaatcgt ctcgaaccgc
     2521 ccctccatct gcgcccgcag ggcggggtcc tgggggagga acaccatgcc gaccccgtag
     2581 tgcccaggcg cggggagcgt gatgccatcc gcggagcaga cccgctgcag gtgagcgtgc
     2641 ggcagttgga tgagcacgcc ggccccgtcg cccgtgtccg gctcggcacc cgatgccccg
     2701 cggtggcaga gtgccaggag gacctccagg ccctgcctca ggacggcgtt ggacttcctc
     2761 ccgtgaatgt cgcagacaaa gccgatgccg catgcatcgt gctcgttggc cgggtcatag
     2821 agcccctgct tccggggcgg gccgtagcat gccatggcgc tgtcgcccat cctccacgta
     2881 caacgttgtg tgccaccgac gccgaaccgg taggcgggac cagagcgccg tcggagggat
     2941 tccaggacgc agcggaaccg aggccggcat gcgcggcagt tggcacaggc cgccccccct
     3001 tgcggatggc ggcctgaaca gtatctatgg cgattatacg atacggaaga gcacttccgc
     3061 aagccccggg tgctacaggc tagctctggg cctcaatccc caagcctcgc gctagccaat
     3121 cgccgaaact acgggcaaga gggacaccag atttccgcat ttctgccgga tccgggtcta
     3181 ccagtcccac gccggggcct aggggtccca tctcagccgc atcacctggt ccagcggcat
     3241 gacctgcacg tgcccatccg cgaagccgat gaccacgctt ccttcatggc gggcggcgag
     3301 ctgctcgggc ccgcccacat tggtgccatc cgcggggtcc ttttcgaaga ccaggaccgt
     3361 ctcggcgggc ttggcgatgt ccgcgagcgt gaggcccagt agcttctcgt tgaacccgta
     3421 tgccggcatc gcatcgcctg ccgcggggca ccgcaatagc tgttcattct tgatgtacgg
     3481 catgagctcc tcgacccagt cctcggttgg gagggtctga tcgtggtcct gagcgtacat
     3541 cattatgccc agcccgatct gcttcgcgtt actcaggcag gcggcctgct cggctttggg
     3601 cctcgctgct ccggctatca gcggaagcag cttcaccaag ccggcgaccg cgctcttctt
     3661 gggccgatag gctacctggc cgtcgactgc catcttattg aggtcaatgc gatacacgac
     3721 accgtcctcg atgacgagca gcttgtcgcc gttcgccacg gccgttggct gcttgccctg
     3781 ggaccccagc gctcgcatga gcatcgcgaa tccgatggcg tcaccatcgc cgccaccacc
     3841 ggggccgccg gccatgccca tcagcatcat gagcatcatc gggtcggcgt cctcctgcgc
     3901 cattgccgtg aacagcgcgc gcatgtctgg cggagccccc agctcctgaa gcatggcctc
     3961 gagttgggca gcttcgtcct gagccgccgg tggttcggcc ggggccactt cggcggccgg
     4021 agcctgg
//
LOCUS       DAOSPL010000219         4027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22461_length_4027_cov_3.182024, whole genome shotgun sequence.
ACCESSION   DAOSPL010000219 DAOSPL010000000
VERSION     DAOSPL010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22461_length_4027_cov_3.182024"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PLQ54_05400"
     CDS             <1..636
                     /locus_tag="PLQ54_05400"
                     /inference="COORDINATES: protein motif:HMM:NF025713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HOS92727.1"
                     /translation="LQALRIAQREPARARHLVDELLSALRVAGLIGSDAAGEDVDRLN
                     RALRSAGWYLTDDGHLQPFGNVDLDTGGRPALDEQLERLRRSTADPALLIGTAKELLE
                     SVSKFVLEELGMPVGNKMSYDQLWHLARERLGVLPQQVDPNLPGVDAIRAIHQSTWNI
                     ADQVNKLRNLQGTGHGRTLPTGVSEDLAMLVVREAATVADYMLARLDREKG"
     gene            638..1474
                     /locus_tag="PLQ54_05405"
     CDS             638..1474
                     /locus_tag="PLQ54_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018741183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92728.1"
                     /translation="MPSQGALFGGPDRDVPKPSRPDELEVLITVKAAPNPSAKAGETV
                     CVAGLGLSRGRTEARWIRLYPINFRFLEQDQKFKKYDIIRVRAVPATGDSRVESWQPD
                     MSTMVVIDHLDPWKRRRALLDPTASDTMCELNRQNQRAAVAPSLGLVPVREVLDLRIT
                     DHAGWTPEEQAKIDAYVNQMDLFDDSDKSPLQAPRLEGHYRWKCYDAQCKGHDQSIID
                     WEFVALQRRLGDSPAAAVRAALKEKFFDMMFKPPRAPAFFVGNQAKRRHVFHVLGVYY
                     PN"
     gene            complement(1462..2070)
                     /locus_tag="PLQ54_05410"
     CDS             complement(1462..2070)
                     /locus_tag="PLQ54_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019547831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HOS92729.1"
                     /translation="MLGSAIAQARREFGITQRQLAAASGVGLETLRKLEQGHVEQPGV
                     FRIAAIADALETTVDALLPARPNRVSSVGYEGCEIATFVDAIQAAGIDLVADVRLTPL
                     SRKQGFSKTRLGAALGDIGVRYEHLRSLGNAKENRPLFAGAELEVGRARYRAGLRTPE
                     ARRALGELASWRQEHHVALLCFERDEERCHRSVVLEELAQLG"
     gene            complement(2310..3095)
                     /locus_tag="PLQ54_05415"
     CDS             complement(2310..3095)
                     /locus_tag="PLQ54_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOS92730.1"
                     /translation="MARPRIKVAFAAGAAVGILGGLIGLGGAEFRLPLLIGLFGFAAL
                     QAVILNKAMSLIVVVTALPARLVSIPYDTLSPYAYVAVNLLAGSLAGAWLGATWATRM
                     RSSTLYRVLAALLVLIAAALAWTHFAHTDPLILTTPARTVLGVVAGVLIGIVAALMGV
                     AGGELLIPTIVLLFAVDIKIAGSLSLAVSLPTMLVAFARYSRDNSFTVLATHKTFVVA
                     MAAGSITGTLLGGLLLGIVPTTILIPVLAALLLASAVKVWHHT"
     gene            complement(3272..>4027)
                     /gene="istB"
                     /locus_tag="PLQ54_05420"
     CDS             complement(3272..>4027)
                     /gene="istB"
                     /locus_tag="PLQ54_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011599313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HOS92731.1"
                     /translation="AAQAAVDAAARELHLPTVREEAIRLADVAVRERRTHLGYLGEVL
                     AAEVDDRQERRRARRVAEAKFPRLKRLADFNVDASTIPAATLAHLASGAYLDAGEPVV
                     MLGDSGTGKSHLLIGLGLAACEQGRRVRYATTAQLVNELVEAADDRALSRVVGKYGRL
                     DLLCLDELGYVHIDPRGAELLFQVITEREERASVAIATNLPFSEWGAVFPDPRLVAAI
                     VDRVTFNAHILETGTESYRLRTSKAMNRRRGQA"
BASE COUNT          693 a         1310 c         1394 g          630 t
ORIGIN      
        1 ctccaggccc tgcggatcgc gcagcgagag ccagcacgtg cccgacacct cgttgatgag
       61 ctgctatctg ccctccgtgt agcgggcctt atcggcagcg acgctgccgg ggaagatgtt
      121 gaccgtctca accgggccct gcgctccgcc ggttggtacc tcaccgacga cggacatctt
      181 caaccattcg gcaatgttga cctggacacc ggcggtcgcc ccgccctgga cgagcagctc
      241 gagcgtctgc gccgctccac tgccgatccc gcccttctca ttggaaccgc caaggagctc
      301 ctcgagtccg tatccaagtt cgtcctcgaa gagctcggca tgccggtggg caacaagatg
      361 tcctacgacc agttatggca cctcgctagg gagcggctcg gggtgctgcc ccagcaggtc
      421 gaccccaacc tgccgggcgt cgatgccatt cgcgcgatcc atcagtcaac ctggaacatc
      481 gccgaccagg tcaacaagct ccggaacctc cagggaaccg gccacggccg gaccctgccc
      541 actggtgtgt ccgaggatct ggcaatgctc gtcgtgcgtg aagccgccac cgtcgcggac
      601 tacatgctcg ccagactcga tcgggagaag ggctgacgtg cctagccaag gtgccctgtt
      661 cggcgggcca gatcgggatg ttccaaagcc ctcccgcccg gacgaactgg aggtcctcat
      721 caccgtcaaa gctgcgccca acccttcagc gaaagcgggc gagacggtat gcgtggccgg
      781 actggggctg tcccgcggcc gcacagaagc gcggtggatc cgcctgtacc ccatcaactt
      841 ccgattcctt gagcaggacc agaagttcaa gaagtacgac atcatccgag tcagggccgt
      901 gcccgccacc ggcgactccc gcgtcgagtc atggcaaccg gacatgtcca ccatggtggt
      961 tatcgaccac ctcgatccct ggaagcgtcg tcgagcactt ctcgacccaa cggcttcgga
     1021 caccatgtgc gaactgaacc ggcagaacca gcgggcagcg gtggcgccat cgctagggct
     1081 cgtccctgta cgggaagtgc tcgatctgcg gatcaccgac cacgccggct ggacaccaga
     1141 agaacaggcc aagatcgacg cctacgtcaa ccagatggac ttgttcgacg actccgacaa
     1201 gagcccactg caggccccgc gacttgaggg tcactaccga tggaagtgct acgacgctca
     1261 atgcaagggc cacgatcaga gcatcatcga ctgggagttc gtcgcgctcc aacgccgcct
     1321 cggcgacagc cccgcggccg ccgtacgtgc cgcgctcaag gagaagttct tcgacatgat
     1381 gttcaagccg ccccgggcac ctgccttctt tgtgggcaac caagccaaac gccgccacgt
     1441 gttccatgtg ctcggtgtgt actaccccaa ctgagcgagc tcctcgagca cgactgagcg
     1501 atggcaccgc tcctcgtccc gctcgaagca gagcagggcg acatgatgct cctgcctcca
     1561 agaggcgagc tcaccaagcg cgcgtcgcgc ctcgggggtc cgaaggccag cccgatagcg
     1621 tgcccgcccg acctccaact ctgcgccggc aaacagtggc cggttctcct tggcattgcc
     1681 caaagaccgc agatgttcgt atcgcacccc gatgtctccg agagccgcgc ccaggcgcgt
     1741 cttgctgaac ccctgcttcc ggctcaacgg tgtgaggcgg acgtcggcga ccaggtcaat
     1801 gccggccgct tggatcgcgt cgacgaacgt tgcgatctcg cagccttcat acccgacgga
     1861 agacacccga ttcggccgag ccggcagcag cgcgtccacg gtcgtctcca gggcgtccgc
     1921 gatggcggcg atccgaaaca ctccaggctg ttcgacgtgc ccctgctcga gctttcgcag
     1981 tgtctctagt cccacccccg aggccgctgc gagttgcctc tgtgtgatgc cgaactcccg
     2041 acgggcttgg gcgatcgcgg acccaagcac ctgactccgg gacggatgaa tggaagactg
     2101 ggggatcacg agagtagtaa agcattacga cctcgacaga gtccgcctcg gggctgagag
     2161 gagcgctcgt gtgggtttcg tcaagttctc tggccccacc tgcggtcacg aatgttggcc
     2221 ccggttcgcg cgtgccggct gacggctggc ggaggatctc gtagcggttg aggttcgact
     2281 ccgggtgggc ggtatcggcg atcccgggct caagtgtggt gccacacctt gacggcggag
     2341 gccagcagga gggccgcgag gacggggatc aggatggtgg tgggcacgat gccgagcagg
     2401 agtccgccga gcagggttcc ggtgatggat ccggcagcca tggccaccac gaaggttttg
     2461 tgggtggcca gcacggtgaa gctgttgtcg cggctgtagc gggcgaacgc gacgagcatc
     2521 gtggggaggg acaccgccag ggagaggctg ccggcgatct tgatgtccac ggcgaagagc
     2581 agcacgatcg tggggatcag cagctcgcca ccggcgacgc ccatcagtgc ggccacgatc
     2641 ccgatcagga cgccggcgac gacgccgagg acggtccggg cgggggtggt caggatcagg
     2701 gggtcggtgt gcgcgaagtg ggtccaggcc agcgcggcgg cgatcagcac tagcagcgcg
     2761 gcgaggactc gatacagggt gctcgagcgc atccgggtgg cccaggtcgc tccgagccag
     2821 gccccggcga ggctgcccgc gaggaggttg accgcgacgt acgcgtaggg gctgagggtg
     2881 tcgtagggga tggagaccag ccgggcgggc agggcggtga cgaccacgat gaggctcatc
     2941 gccttgttca ggatgacggc ctgcagcgcg gcgaacccga acagcccgat cagcagcggt
     3001 aggcggaact ctgcgccgcc gagcccgatc agccctccaa ggatcccgac cgcggcaccg
     3061 gcggcgaaag ccaccttgat ccgtggtcgg gcgatggtgg gtgtgccccc gggtggactc
     3121 acgggtggtt cctgcgaagg gacgtggggg gccgcatcag ctggcagggg gccgtgcagg
     3181 tcggacatgc tggggacagt accggcgacg ctcgcgacgt cgcacccggt gggccgtcga
     3241 gcggcagccg cgtgaatggc cccgaccggg gtcaggcttg tccccgtcgt cggttcatcg
     3301 ctttgctggt gcgcaggcgg taggactcgg tgccggtctc gaggatgtgg gcgttgaagg
     3361 tgacgcggtc gacgatggcg gcgacaaggc gggggtcggg gaagacggcg ccccactcgg
     3421 agaacggcag gttggtcgcg atcgcgacgg acgcgcgttc ttcgcgttca gtgatgacct
     3481 gaaagagcag ctccgcgccg cggggatcga tgtggacgta gcccagctcg tccaagcaga
     3541 gcaggtcgag gcggccgtac ttgcccacga cccgggacag ggcgcggtca tcggcggctt
     3601 cgacgagctc gttgaccagt tgtgcagtcg tggcgtaccg cacccgccgg ccctgctcgc
     3661 acgcggcgag gccgagcccg atcagcaggt gcgacttgcc ggtgccggag tcaccgagca
     3721 tgaccacggg ttccccggca tcgaggtacg ccccggacgc gaggtgcgcc aacgtggcgg
     3781 ccgggatggt cgaggcgtcg acgttgaagt cggccaggcg tttgaggcgg gggaacttcg
     3841 cttccgcgac ccggcgggcc cggcggcgtt cttgccggtc gtcgacctcg gcggcgagga
     3901 cttccccgag gtatcccagg tgggtgcggc gttcccggac ggcgacgtcg gccaggcgga
     3961 tcgcctcctc tcgaacggtc gggaggtgca gctcgcgggc ggcggcgtca accgcggcct
     4021 gggcggc
//
LOCUS       DAOSPL010000220         4027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22462_length_4027_cov_3.151561, whole genome shotgun sequence.
ACCESSION   DAOSPL010000220 DAOSPL010000000
VERSION     DAOSPL010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22462_length_4027_cov_3.151561"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLQ54_05425"
     CDS             <1..220
                     /locus_tag="PLQ54_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92732.1"
                     /translation="ILFPTQLLSWESTAAVQIWASAVTQVADIALTPLVLAGAAVFYF
                     DQRCRVEALDLLAAFRSVRARSSEGRSP"
     gene            217..960
                     /locus_tag="PLQ54_05430"
     CDS             217..960
                     /locus_tag="PLQ54_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HOS92733.1"
                     /translation="MTRLLGLLAALLLLDAATGQEAPSAPGPEAWPSERAAPQAIKEA
                     ARQVLQSREFATDLRSAALRQVYERIVQAIRERLARLAGFLSASSRIAAVAAIVMAAL
                     CVVVAAYVIWLAVRGLPRARTAGGGSAASADEGEAEVIEQAFPSSAELRRRASALHTA
                     GDLTGAVRWYFQAYLSLLHELGLVFLDPRRTNAEYVGSLGDDTEHGPRLREAVERFER
                     VWYGGQPGKARDVEALEVLSRPAVGRAQA"
     gene            957..2174
                     /locus_tag="PLQ54_05435"
     CDS             957..2174
                     /locus_tag="PLQ54_05435"
                     /inference="COORDINATES: protein motif:HMM:NF025617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HOS92734.1"
                     /translation="MNALRRYRAALAGLVLLVAVQTVAVVWNPGGRMDAVPDRSTYRF
                     NRFGYCAAYRLLGRLGYPVERNLQSLDLLPDRIRQLWLVNPMVPLEAKELRRLRAWVS
                     AGGTLVCAFDSALTADDGTEAPDAFPELTEFRVRGGPYAMLGLLEAPGLVPPEPKVVD
                     YAAGAAYGTDVVRIAVPHGARLDGNAADRWRSVRDEQGTVLARRPVGEGNLVVTSETD
                     LFCNANLAREDNAVLIANLAHAAGGPIAFDEYHHGFRSLRSPGDALGVFGGKVGVYQL
                     AVAATMMLVASSRRLGSPRRLKPPSRRSALEFVDSMAALYQLSGARDTALQSVGRGAR
                     RRLARRLGLPMTSSDEEIAARIRLLEPHGTLRPDEAIGLLARSAEPVANDAQLVAVAR
                     RLDDLGGGGRPVG"
     gene            2167..3126
                     /locus_tag="PLQ54_05440"
     CDS             2167..3126
                     /locus_tag="PLQ54_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018517854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOS92735.1"
                     /translation="MASLEAVWITGEAIRNEIAKAVVGQEDVIEHLLIGLLSGGHVLL
                     EGVPGTAKTLMVKALALSVGCEFKRVQMTPDLMPSDILGVSIYDRAADRFTLRKGPVF
                     TNLLLADEINRAPAKTQAALLEAMEEGVVTIDGEDHRLSPPFMVCATQNPIEHEGTYM
                     LPEAQLDRFMFKVLVPYPPEAVEAQMLQRHHQGFDPRRLGDLGLSPKADGHTIESCRI
                     ALRDVQVHDDVARYVLAIVRGTRERQFLSLGGSPRASAVLLMDSKARAALHRRTFVTP
                     DDVKAMAKPTLRHRVLLEPEAELDGLTPDRVIDGIVSQIPVPR"
     gene            3138..>4027
                     /locus_tag="PLQ54_05445"
     CDS             3138..>4027
                     /locus_tag="PLQ54_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOS92736.1"
                     /translation="MPFPTQRLALLVAVAGLLPLAAGRVPGALPAGLALDAALVALVI
                     VDALRSVRRHNLAAFRALPTRLCTGQPNRVTLRIENRSRRAVRGVAVDDCPIDLATDR
                     ELLPWEAGAGTRVSLRYTVTASGRGEFAFGDVHLRWLSPWGLLSWQAKWPGATPVRVY
                     PRYVDFSRYHLEYRSTLLREAGPQRIRSVVRGGEFESLRDYVPGDDTRQIDWKATARH
                     QRLTVRNHDEERSKSVLLVLDAGRMMSPVSGGLSKLDHAINAALMLCSAAITKGDRVG
                     LMVFDEAVRLYLPPRTGVAQ"
BASE COUNT          571 a         1305 c         1448 g          703 t
ORIGIN      
        1 gatcctgttt cccacccagc tcctgagttg ggagtcgacc gcggcggtac agatctgggc
       61 cagcgccgtg actcaggtgg ccgacatcgc cctgacccct ctggtcctgg caggggccgc
      121 agtgttctac ttcgatcagc ggtgccgcgt ggaggccctt gacctcctgg cggcattccg
      181 gtcggtccgc gcgcggtcga gcgagggtcg gtcgccatga cacggctgct gggcctgctc
      241 gccgcgctgc tgctgctcga cgctgcaacg gggcaggagg cgccgagtgc gcccggaccg
      301 gaggcctggc cctcggagcg cgccgcacca caggcgatca aggaggccgc gcgacaggtg
      361 ctgcagtcgc gggagtttgc caccgatctg cgttcggcag cgttgcgcca ggtctacgag
      421 cggatcgtcc aggccattcg ggagcgtctg gcgcgtctgg cggggttcct atcggcatcg
      481 tccagaatcg ctgcggtggc cgcgatcgtg atggcggcgc tctgcgtggt ggtggccgcg
      541 tacgtcatct ggctggctgt gcgcgggctt ccacgtgccc gcacagcggg gggtggctcg
      601 gcggcctccg ccgatgaggg ggaggccgag gtcattgagc aggccttccc gtcctcggca
      661 gagctgagac gccgggcatc agcactgcac acagcgggtg atctcactgg ggcggtgagg
      721 tggtactttc aggcttacct gtcgctgctg cacgaactcg gcctggtgtt cctggatccg
      781 cgccgcacaa acgccgagta cgtgggctcg ctgggcgatg acaccgagca tggtccgcgc
      841 ctgcgcgagg ccgttgagcg gtttgagcgc gtctggtacg gcggccagcc cgggaaggcg
      901 cgggatgtgg aggccctcga ggtgctatcg cgccctgcag tggggagggc gcaagcgtga
      961 atgccctgcg ccggtaccgt gcggctttgg ccgggctggt cctactcgtc gcggtgcaaa
     1021 ctgtcgccgt ggtctggaac ccgggtggcc ggatggacgc cgtgcctgac cggtcgacat
     1081 atcgcttcaa ccggttcggg tactgcgccg cctaccgact gctcggccgt ctggggtacc
     1141 cggtggagcg gaacctgcag tcactggacc tgcttcccga tcggatccgt cagctctggc
     1201 tcgtgaaccc gatggtgccc cttgaggcca aggagcttcg gcggctccga gcatgggtct
     1261 cggctggggg gacgctggtg tgcgcgttcg acagtgccct gacggccgac gacggaactg
     1321 aggccccgga tgcgttcccc gagctgacgg agttccgcgt gagaggcggc ccgtatgcca
     1381 tgctgggcct cctggaggcg ccggggctcg ttccacctga acccaaggtg gtcgactatg
     1441 cggccggcgc ggcctatggt accgacgttg tgcgcatcgc ggtgccgcac ggggcgcggc
     1501 tcgatggcaa tgccgccgat cgatggcggt cggtgcgcga cgagcagggc acggttctgg
     1561 cccggcgccc ggttggtgag ggcaatctgg tggtgacgag cgagactgac ctgttctgca
     1621 acgccaacct tgcgcgcgag gataacgccg ttctcattgc caatcttgca cacgcggcgg
     1681 gcggccccat cgcattcgac gagtaccacc acgggttccg ctccctgcgc tcgcccggcg
     1741 acgcgctggg cgtgtttggc ggcaaggtgg gtgtgtatca gctggcggta gctgccacga
     1801 tgatgctggt ggcctcttcc cgccggctgg gctcgccgag gaggctgaag cctccgtcgc
     1861 gccggtcggc gctggagttc gtggactcga tggcggccct gtaccagttg tcaggggccc
     1921 gggacaccgc ccttcagtcc gtgggccgcg gcgcccggcg acgcctggca cggcgtctgg
     1981 gactaccgat gacatcatct gacgaagaga tcgctgcgcg catccggctg ctggagcccc
     2041 acgggacact ccgcccggac gaggccatcg ggctgctggc gcgctccgcc gaacctgtcg
     2101 ccaacgatgc gcagctcgtg gcggtggcac ggcgactgga tgacttggga gggggcggac
     2161 gacccgttgg ctagcctcga ggctgtctgg attaccgggg aagcgatccg caacgagatc
     2221 gccaaagccg tggtgggcca ggaggatgtg atcgagcatc ttctgatcgg cctactttcg
     2281 ggtgggcatg tgctcctcga aggcgtgccc ggcacagcga agacgctgat ggtgaaggct
     2341 ctggccctgt cggtaggctg tgagttcaag cgggtgcaga tgaccccgga cctgatgccg
     2401 tcggacatcc tcggggtgtc gatctacgac cgggcggcgg accgtttcac gctgcggaag
     2461 ggcccggtct tcacgaacct gctgctggcc gatgagatca atcgggcccc ggcgaagacc
     2521 caggccgccc ttctcgaggc catggaggag ggagtggtca ccatcgacgg tgaggaccat
     2581 cggttgtccc cgccgttcat ggtctgcgcg acacagaacc ccattgagca tgaggggacc
     2641 tacatgctcc cggaggcaca gctggaccgg ttcatgttca aggtgctcgt cccctacccg
     2701 cccgaggcag tcgaagcgca gatgctccag cggcatcacc aaggattcga cccgcgtcgg
     2761 ttgggtgacc tgggtctgtc ccccaaggcc gatggccaca cgattgagtc ctgccgcatc
     2821 gccctgaggg acgtccaggt gcatgatgat gtggcccgct acgtgctggc gatcgtgcga
     2881 ggcacgcggg agcgccagtt cctgtcgctg ggggggagcc ctcgagccag tgccgtgctg
     2941 ctgatggaca gcaaggcccg cgcggcgctg catcggcgca ccttcgtgac tccagatgac
     3001 gtcaaggcga tggcaaagcc cacgctgcgc caccgcgtgc tgctcgagcc cgaggcggag
     3061 ctcgatggcc tcacgccgga ccgcgtgatc gacggcatcg tctcgcagat ccccgtgcct
     3121 cgctgaggag cgctgccgtg ccgttcccga cacagagact ggccctgctg gtcgccgtgg
     3181 ccggactgct cccactggcg gcgggccgag tgcctggcgc cctgcccgcc ggcctggcgt
     3241 tggatgccgc actggttgcg ctggtgattg tggatgcctt gcggtcagtt cgtcggcaca
     3301 acctggcagc cttccgcgcc ctgccgacgc gcctctgcac aggccaaccg aaccgcgtga
     3361 cgctccgcat cgagaaccgt agccgacgag ctgtccgcgg cgtggccgtc gatgactgcc
     3421 ccatcgacct cgccaccgac cgtgagctgc tcccctggga ggcgggagcc gggacgcgcg
     3481 tctcactccg ctacacggtt acggcatcgg gccgcgggga gttcgccttt ggcgatgtcc
     3541 acctgcgttg gctcagtccg tgggggctgc tctcgtggca ggccaagtgg cccggtgcga
     3601 ccccggtccg cgtctatccg cgctatgtgg atttctccag ataccatctt gagtatcgct
     3661 cgacgctctt gcgggaggcc ggtccgcagc gcatccgcag cgttgtgcgc ggcggcgagt
     3721 tcgagagcct tagggactat gtccctggcg atgacacccg ccagatcgac tggaaagcca
     3781 cggcccggca ccagcggctg accgtgcgca accacgacga ggagcgcagc aagagcgtgc
     3841 tgctggtcct ggacgcgggc cggatgatgt cgccggtgtc gggcggtctg tcgaagctcg
     3901 accacgccat caacgcggcc ctgatgttgt gttcagccgc gatcaccaag ggcgaccggg
     3961 tcggcctgat ggtgttcgat gaggcggtgc ggctctacct gccgccgcgg acgggtgttg
     4021 cgcagag
//
LOCUS       DAOSPL010000221         4025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22479_length_4025_cov_3.190932, whole genome shotgun sequence.
ACCESSION   DAOSPL010000221 DAOSPL010000000
VERSION     DAOSPL010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22479_length_4025_cov_3.190932"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..1073
                     /gene="motA"
                     /locus_tag="PLQ54_05450"
     CDS             216..1073
                     /gene="motA"
                     /locus_tag="PLQ54_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HOS92737.1"
                     /translation="MFTIVGLGVVMASLVIGFTMAGGKIPVLIQINEYIVILGAATGS
                     VIIGSGIGGLTKLIQTVLGLLKPDPFGKAQFTALLRMLYEIVSQTRREGLVSLEAHID
                     KPSQSAIFKRYPAFLACHHALSFLTDGLRLIVDGIDPFMLDELMQADMQARHAESIRP
                     AQQLQKLGDAMPGYGIVAAVLGVVITMGHVSESAEVVGHHVASALVGTFLGVLMAYGV
                     LQPLSSAAEARASSEHQYLECIRSAMLAMARGDNPFLAVDFARRAVEPELRCSFEELE
                     KAVKSAGSA"
     gene            1089..1952
                     /locus_tag="PLQ54_05455"
     CDS             1089..1952
                     /locus_tag="PLQ54_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOS92738.1"
                     /translation="MKGGGGDGQRPIIVIRRRGGHEGHHGGAWKVAYADFVTAMMAFF
                     IVMWIMGMSPEVKSAISGYFQDPTGFGKQGPGGLLIGAQGGEKKVQLIELPAKERFAI
                     AKRELEKKLNASPEFGKVKDSVDITVTDDGLRVELLEGEQSLFFELGSAEMKPQTADL
                     LRMIAKELAKLPDRLAIEGHTDSVGYSGSRPDYSNWELSADRANAARRAMIADLREGQ
                     LSAVRGLADTQLRDPEHPRSPSNRRVSILVEPKELMKASSVSVDAKTAANLGGRSSSG
                     ESGGATSGAGE"
     gene            2228..2614
                     /locus_tag="PLQ54_05460"
     CDS             2228..2614
                     /locus_tag="PLQ54_05460"
                     /inference="COORDINATES: protein motif:HMM:NF021568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HOS92739.1"
                     /translation="MTRSEAEARIAESLARLEHEFTGRATDDVVAHIIGDLLVARLRG
                     GVAPAERHLVESSDRARGRALVKNAHFELVDRARPLIEATIHGLLSRRVVSLHTDIST
                     TTGERILVASLESAPEFEPEGTHPEA"
     gene            2625..>4025
                     /locus_tag="PLQ54_05465"
     CDS             2625..>4025
                     /locus_tag="PLQ54_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92740.1"
                     /translation="MAFKRFDLLGRQVILHTEGLICSSPREMLGSSVFRRIVSLFCDS
                     LRERQSPLIEPFEPPFVAGQDWMGVADFLRAVAENPIETIARILPGTEHMTAPGTRAV
                     LNDFVQELYNFWRRFDRYVVLHSEPGPTSMDRRPYRAFNSTIEGLGHIVRAAYRDIAE
                     NITGDHPNVYRQVAAGCNIGLIATPRTLPLPSPYAELLDGIPVIRQVWMTPPMIMDPP
                     TNTRSGQFVRVDENPLGRFRPERTRWLCYPARVGPMVVHVCFHAQFMGLGCALANLFE
                     LATDDQLAQPASALYFYGVPPEELAGLGDSQTVFFDDETAGLLVAAVPGAERYGYFGY
                     LKKMILTLHNASAMKQGLMPFHGAMVRIALRDGPEANVLIIGDTATGKSESLEALRLL
                     GGSRVRRIRVVADDMGSLRIGGDGRLLAYGTEIGAFVRLDDLQQGYVFGQVDRAIIMS
                     PHRTNARVVLPVTTLAE"
BASE COUNT          659 a         1282 c         1370 g          714 t
ORIGIN      
        1 cgccaccgcg accgcacgga cccgagtcgc tggcccggcc acattcgcga ccaaagcact
       61 gagtatggtt gggtgcgtgt ggaataggtt gagtgtatcc ggccgctggg gtgcggtggt
      121 ggcgaggtgg tgacggccgg cgacgcccgc tgagagcgcg gcgcctcctt gggtgctgcc
      181 tcacaggacc gggccggcga ggtgagagag agactatgtt taccattgtc gggcttggcg
      241 tcgtcatggc cagtctggtc atcggcttca ccatggccgg cggcaagatt ccggttctga
      301 tacagatcaa cgagtacatc gtcatcctgg gggccgccac cggttcggtg atcatcggca
      361 gcggcattgg cgggctcacc aagctgatcc agacggtgtt gggactgctc aagccggatc
      421 ctttcggcaa ggcccagttc accgcgctac tccggatgct ctacgagatc gtctcccaga
      481 cgcggcgtga gggtctggtc tcgctggaag cgcacattga caagccctct cagagcgcca
      541 tcttcaagcg ctaccccgct ttcctggcct gccaccatgc gctgagcttc ctgaccgacg
      601 gactccggct catcgtggac ggtatcgacc cgttcatgtt ggacgagctc atgcaggccg
      661 atatgcaggc gcggcacgcc gagtcgattc ggccggccca gcagctccag aagctggggg
      721 acgcgatgcc ggggtacggc attgtggcgg cggtgctcgg cgtggtgatc accatgggcc
      781 atgtcagcga gtcggccgaa gtggtcgggc accacgtggc ctcggctctg gtgggcacct
      841 ttctgggcgt cctgatggcc tatggcgtcc tgcagcccct ctcatcggca gccgaggcga
      901 gagccagctc ggaacaccag tatctcgagt gtatccgatc ggcgatgctg gccatggcac
      961 gtggcgataa cccgttcctg gccgtggact tcgcgcgtcg tgccgttgag ccggagctgc
     1021 ggtgctcctt cgaagagctt gagaaggccg tcaagtcggc gggatcggcg tagggggagg
     1081 gctgagccat gaagggcggc gggggagacg ggcagcgccc catcatcgtt atccggcgcc
     1141 gcggcggcca cgagggccac catggcgggg cctggaaagt ggcctacgcc gacttcgtta
     1201 cggccatgat ggcgttcttc atcgtcatgt ggatcatggg catgagcccg gaggtcaaga
     1261 gcgccatctc ggggtacttc caggacccga ccggtttcgg gaagcagggg ccgggtggtc
     1321 tgctgattgg cgcacagggc ggcgagaaga aggttcagct catcgagttg ccggccaaag
     1381 agcgtttcgc catcgcgaag cgtgagctgg agaagaagct caatgcctct cccgagttcg
     1441 gcaaggtcaa ggattcggtg gacatcaccg tgaccgacga tggtctgcgg gtcgaactcc
     1501 tcgagggtga gcagtcgcta ttcttcgagc tgggcagtgc ggagatgaag cctcagacgg
     1561 cggatctgct gcgcatgatc gccaaggaac tcgccaaact ccccgatcgc ttggccatcg
     1621 agggtcacac cgacagtgtg gggtactcgg ggagcaggcc ggactactcc aactgggagc
     1681 tctcggccga ccgggccaac gcggcgcgaa gggcgatgat cgccgatctc cgcgagggcc
     1741 agctctcggc cgttcgggga ctggccgaca cccagcttcg cgacccagag cacccgcgca
     1801 gcccgagtaa ccgccgcgtg agcatcctgg tcgagcccaa ggagctgatg aaggcgtcgt
     1861 cggtcagcgt cgatgccaag accgccgcta acttgggcgg tcgatcgtct tccggcgagt
     1921 cgggcggcgc cacatccggg gcgggtgagt gaaccgtcgg ggcggtcgcc gacacccccc
     1981 atcgacggtg acttggggct ccatcggacc gtgggcctgg ttgcggagcg gcgccatggg
     2041 aggaaccatg cgggcggtgc ggcgtacggg cttgccgcac acagcccggg ctcaagtgac
     2101 cgcccatgcg gacacccccg acgcccctgc ttcggtatcg cgcgagcggc gcccgccttg
     2161 cccgccggtg ctccctctgc tagagtttat gctctagcac cttccaccgg cgggagcacc
     2221 gatgacgatg acgcggagtg aggctgaggc ccggatcgcc gagagcttgg cccgcctgga
     2281 gcacgagttc acgggccgcg cgaccgatga cgttgtcgcc catatcatcg gcgaccttct
     2341 ggtggcccgc ctacgcggcg gcgtggcgcc tgccgagcgg catctggtcg agagcagcga
     2401 tcgggcccgg ggccgggcgt tggtgaagaa tgcgcacttc gagctggtgg accgcgcgcg
     2461 gccgttgatt gaggccacga tccacgggct gctctcgcgg cgcgttgtct cccttcacac
     2521 cgatatcagt accaccacgg gggagcgcat tctggttgcc tcgctggagt ccgcgcccga
     2581 gttcgagccc gaggggaccc atcccgaggc ttagtggagt cgccatggca ttcaagcggt
     2641 tcgatcttct gggacgtcag gtcatcctcc acacggaggg cctgatctgc tcaagccccc
     2701 gcgagatgct gggaagctcg gtgttccggc ggatcgtcag tctcttctgc gacagcctgc
     2761 gggagcggca atcaccgctg atcgagccgt ttgagccccc cttcgtggcc gggcaggact
     2821 ggatgggagt cgcggacttc ctccgagccg tcgccgagaa ccccatcgag accatcgcgc
     2881 gcatcctgcc agggaccgag cacatgacag cacccggcac acgggccgtg ctgaacgact
     2941 tcgtccagga gctgtacaac ttctggcggc gattcgaccg gtatgtggtt ctgcattcgg
     3001 agccggggcc caccagcatg gaccggcgtc cctatcgcgc cttcaactcg accatcgaag
     3061 gtctgggcca cattgttcgc gcggcgtacc gtgacatcgc tgagaacatc accggagacc
     3121 atcccaatgt ctaccggcag gtggcggcgg gatgcaacat cggcctcatt gctacaccgc
     3181 ggacgctacc cctgccgtca ccctatgccg agctgctcga cgggatcccc gtcatccggc
     3241 aggtgtggat gacacccccc atgatcatgg atcccccgac gaacacccgg tcgggccagt
     3301 ttgtgagggt cgacgagaac cctctcggcc ggtttcgccc ggagcgcacc cggtggctct
     3361 gttacccggc ccgagtcgga cccatggtgg tccacgtgtg cttccacgcc caattcatgg
     3421 ggcttggttg cgccctcgcg aacctgttcg agctggccac cgacgatcag ctcgcccagc
     3481 cagccagtgc gctgtatttc tacggcgtgc cccccgagga actcgccggc ctgggcgatt
     3541 cccagacggt gttcttcgat gacgagaccg cggggctgct ggtggccgcg gtacccggcg
     3601 ccgagaggta tggctacttc ggttatctca agaagatgat cctcaccctt cacaatgcgt
     3661 cggcgatgaa gcaggggctg atgccattcc atggcgccat ggttcgcatc gcactgcgcg
     3721 atggccccga ggcgaacgtg ctcatcatcg gcgacacggc gacgggcaag tccgagtcgc
     3781 tggaggccct gcggctgctg ggtgggtcgc gggttcggcg aattcgggtc gtagccgacg
     3841 acatggggtc gctccgcatt ggcggcgatg gccgcctgtt ggcctacggc acggagatcg
     3901 gcgctttcgt gcggctcgat gacctccagc aggggtatgt gttcggccag gtggaccgcg
     3961 caatcatcat gagcccgcat cgaaccaatg cgcgcgtcgt gctcccggta accacgctgg
     4021 ccgag
//
LOCUS       DAOSPL010000222         4015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22554_length_4015_cov_2.904545, whole genome shotgun sequence.
ACCESSION   DAOSPL010000222 DAOSPL010000000
VERSION     DAOSPL010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22554_length_4015_cov_2.904545"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLQ54_05470"
     CDS             complement(<1..183)
                     /locus_tag="PLQ54_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92741.1"
                     /translation="MLDSGLDLIIAMSYHQADAQLTAPPPVLPRCFRIDVLRRGLWET
                     HARITRNHQRLVRVPVG"
     gene            complement(147..1676)
                     /locus_tag="PLQ54_05475"
     CDS             complement(147..1676)
                     /locus_tag="PLQ54_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS92742.1"
                     /translation="MTAPRVALRHSLHRVDVCVVGGGMAGICAALAAARHGARVALLH
                     DRPVLGGNASSECRVHICGADRHGGIPNLRETGILEELRLENLARNPQRSFSVWDTVL
                     YEAVRLQPNLDLLLNCSVLDARMRHGAIGSVTGWQLTTETHHTVQADVFIDCSGDAIL
                     APLTGAAFRVGRESRHEFGESIAPEEADAKTMGMTCLFQARRHESPQPFEPPPWAYRF
                     ETDSELPYGTGGHRWWEMGYWWVELGGEHDSIHDTEALRDELLRILYGVWDHIKNRGD
                     HGADCWALEWVQFLPAKRESRRYEGELMVTQGDLESGGRFIDTVAYGGWAMDDHHPAG
                     FWSVRIGEPSTQFHPTPSPYGIPLRALISRDVANLMFAGRCASFTHAAMSSARVMGTG
                     AVMGQAAGTCAAMAAASGVRPSRMVGRAPDLQQALLRDDCYLPGVPEHLPELTRAARL
                     EASTGDPSPCSPASTAPWATTGTRGPVPLATTCRCASSSLVTLARLDSCWIAGSTSSL
                     P"
     gene            complement(1699..>4015)
                     /locus_tag="PLQ54_05480"
     CDS             complement(1699..>4015)
                     /locus_tag="PLQ54_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOS92743.1"
                     /translation="PLLLEECRDNADVLVWPGFAPFRDVIARAVGGQRTALTPANRVT
                     VLLRQPWDLPRIERPANQAPPRPDEVRELLSPGGRVAELHPDYEHRPEQIAMAERVTR
                     AFLDDEVLLVEAGTGVGKSLAYLIPALLWASRTGQRVAVSTATKNLQEQLVRKDIPFL
                     EKVLGFPFRATVVKGRENYVCARKFAEAYERARNSMLTEDRLAALKTLAWACLSSNRD
                     LSSVGEARGGGLAAGEHFAAALRSDGETCHRRACRYNAACAVERLRRVALASDLLILN
                     HALLFAAGPSTGVLPPFSRLVFDEAHLVEDVCTEFLGLTFSARQCQRVLNRLGHRGDP
                     LALLHRLEADVQRSPEALANQIDPTVLGELLSSVEAAAASSRALVDLASELTRELTGR
                     EEASRMRLKPESFAGEAGSAFAEAAVDLAGELRLVSGQARGLAKRFEEAGDELDVADA
                     QGYANELQGLADSLQEMASAVEIIVAQEDGGYVYWADVERGDDELHAAPIDVGPLLAD
                     GILAEMKTVVLTSATLTVEGDAGFFRQRLGLDRLGQGRLACEVFPSSFDYPNQAILAV
                     PTDAPLPTEDEWLSYTVRAIIDLLVASQGRGLVLFTARSVLERAYEACVGPLTAAGIP
                     VLCQYRDGTRAALQAELIRDKASVLFATRSFFAGVDVPGDSLECVILTKLPFAVPSDP
                     VIQARCESLEAEGIDAMSEYYIPQAIVTFRQAFGRLIRSRKDRGVIAVLDRRLLSRPY
                     GRRFRASIPQCGEVLCETAGMIARVQRFLRS"
BASE COUNT          702 a         1402 c         1335 g          576 t
ORIGIN      
        1 gccaacaggc acgcggacca gccgctggtg gttgcgggtg atgcgcgcat gggtctccca
       61 caggccccgg cggaggacat caatccggaa acaccggggg aggacgggtg gtggcgccgt
      121 gagctgggcg tcggcctggt ggtagctcat ggcaatgatg aggtcgagcc cgctatccag
      181 cacgagtcca acctggccaa cgtgacgagg ctcgacgaag cacagcgaca cgtggtcgcc
      241 aatggcacag ggccacgagt tccggtcgtc gcccacgggg cggttgatgc cggtgagcaa
      301 ggcgagggat cgcccgtcga cgcttcgaga cgcgcggccc gcgtcagttc gggcaagtgc
      361 tcgggcacgc cggggaggta gcagtcgtcg cggagcaggg cctgctgcag gtcgggcgcc
      421 cggcccacca ttcgcgaggg tctgacgcca ctggcggcgg ccatggcagc acaggtgccc
      481 gcggcctggc ccatgacggc gccggtgccc atcacccgcg ctgagctcat ggccgcgtgc
      541 gtgaagctgg cgcaccggcc ggcgaacatc aggttagcca catctcgcga gatcagtgcg
      601 cgcaacggga tcccataggg cgagggtgtg gggtggaact gggtcgatgg ctcgccgatc
      661 cgcacggacc agaacccggc cgggtggtga tcatccatgg cccatccgcc gtaggcgacg
      721 gtatcgatga accggccccc ggactccagg tcgccctggg tgaccatgag ttccccctcg
      781 taccgtcggc tctcgcgctt ggcgggtagg aactggaccc actcgagcgc ccagcagtcg
      841 gcgccgtggt ccccgcggtt cttgatgtgg tcccatacgc cgtagaggat ccgcagcagc
      901 tcatcgcgga gcgcctcggt gtcgtggatg ctgtcatgct cgccgcccag ctcgacccac
      961 cagtagccca tctcccacca tcggtgccct ccggtaccgt acggcagctc ggaatcggtc
     1021 tcgaagcgat aggcccaggg cggcggctcg aagggctgtg ggctctcgtg acgccgtgcc
     1081 tggaacaaac aagtcatgcc catggtcttc gcatcggcct cttcgggcgc gatcgactcg
     1141 ccgaactcgt gccgcgactc gcggccgacc cggaaggcgg caccggtcag gggcgccaga
     1201 atcgcgtcac ccgaacagtc gatgaacacg tctgcctgca ccgtgtggtg ggtctctgtc
     1261 gtgagctgcc atccagtgac gctgccgata gccccgtggc gcatccgggc atcgagcaca
     1321 ctgcagttca gcagtaggtc gagattcggc tggagccgca cggcctcgta taggacggta
     1381 tcccacaccg agaaactgcg ctgaggattg cgggccaggt tctcgagccg cagctcctcc
     1441 aggatgccgg tctcgcgcag gttcgggatg cccccgtgcc ggtcggcgcc acagatgtgg
     1501 acccggcact cactggaggc attcccgccc agcacgggcc ggtcatggag cagggcgaca
     1561 cgagcaccgt ggcgcgccgc ggccagggca gcgcagatac ccgccatgcc accgccgacc
     1621 acacagacat cgactcggtg caggctgtgc cgcagcgcaa cacgtggcgc ggtcatgagg
     1681 tgcacccccg cggtcgagct acgaacgcag aaagcgctgg acgcgggcga tcatgcccgc
     1741 ggtctcgcac aggacctcgc cacactgggg aatcgaagcc cggaaccggc gtccgtacgg
     1801 ccgggacagg agccgtcggt ccagcaccgc gatcaccccc cgatctttcc tcgaccggat
     1861 caggcggcca aaggcctgcc ggaacgtgac gatggcctgc gggatgtagt actcgctcat
     1921 ggcatcgatg ccttcggcct ccagcgactc gcaacgcgcc tggatgaccg ggtcactcgg
     1981 aacggcgaag gggagcttgg tcaggatgac gcactcgagg gaatcccccg gcacgtccac
     2041 cccggcaaag aaactacggg tggcgaaaag caccgaagcc ttgtcacgga tcagctccgc
     2101 ctgcagtgcg gctcgcgtgc cgtcgcggta ctggcacaag acgggaattc cggccgccgt
     2161 caacggaccc acacaagcct cataggcccg ctccagcacg ctcctcgcgg tgaacagcac
     2221 cagaccgcgg ccttgactcg cgaccagcag gtcgatgatg gcccgcactg tgtagctcaa
     2281 ccactcatcc tcagttggca ggggggcgtc cgtcggaacg gccagaatgg cctgattggg
     2341 gtaatcgaaa ctcgacggga acacctcgca ggcgagacgc ccctggccga gacggtccag
     2401 gcccagccgt tgccggaaga acccggcatc tccctccacc gttagtgtag ccgaggtcag
     2461 caccaccgtc ttcatctcag cgaggatccc gtcggccaga agaggaccca cgtcgatggg
     2521 cgcggcgtgg agctcgtcat cgccccgttc gacgtcggcc cagtacacgt accccccgtc
     2581 ctcctgtgcg acaatgatct cgaccgccga agccatctcc tggagcgagt cggccagtcc
     2641 ctgcaactcg ttggcatagc cctgtgcatc ggccacatcg agctcgtcac cggcctcctc
     2701 gaagcgcttc gccaggcccc gggcttgccc ggacacgaga cgcagttcgc ccgccaggtc
     2761 cacggccgcc tcggcgaacg ccgaccccgc ctccccggcg aacgactctg gcttcagcct
     2821 catgcgcgag gcctcctcgc ggcccgtgag ctcccgggtc agttccgagg cgaggtcaac
     2881 cagggcgcgt gaagacgccg ccgcggcctc gacggacgac agcagctcgc ccagaacagt
     2941 gggatcgatc tgattcgcca aagcctccgg gctccgctgg acgtcggcct cgagccgatg
     3001 aagcagagcc aggggatcgc cgcggtgccc gagccgattg agcacgcgct ggcactggcg
     3061 tgccgagaag gtcaggccca ggaactccgt gcacacgtcc tcgaccaggt gggcctcatc
     3121 gaagaccaat cgcgaaaagg ggggcaggac cccagtggac ggcccagcgg cgaagagcag
     3181 cgcgtgattc aggatcagca ggtcgctggc cagtgcgact cgccgaagcc gctcgaccgc
     3241 gcaggccgcg ttgtagcggc aggcccggcg gtggcaggtc tcaccgtccg accggagggc
     3301 cgccgcgaag tgctcccccg ccgccagccc cccaccgcga gcctccccca ccgaagacag
     3361 gtccctgttg gacgagagac acgcccacgc cagtgtcttc agggcggcca gccggtcctc
     3421 cgtgagcatc gagttccgcg cccgctcgta agcctcggcg aacttccggg cgcagacgta
     3481 gttctcgcgg cccttgacca ccgtcgcgcg aaaggggaag cccagaacct tctcgaggaa
     3541 gggaatgtcc ttccgaacca gctgctcttg caggttcttg gtggccgtcg aaaccgccac
     3601 ccgctgcccg gtccgactcg cccagagcag ggcgggtatg agataggcca gcgacttgcc
     3661 aacacccgtg cccgcctcca caaggagcac ctcgtcatcg aggaacgccc gggtgacgcg
     3721 ctcggccatg gcgatctgct cgggccggtg ctcatagtcc gggtgcagct ccgcgactcg
     3781 gccgccgggg ctgagcagtt ccctgacctc atcggggcgc ggcggcgcct gattcgcggg
     3841 ccgctcgatg cgcggcagat cccacggctg gcggagcaaa acggtcacgc ggttcgccgg
     3901 cgtaagagcg gttctctggc cacccacagc gcgggcgatg acgtctcgga agggggcgaa
     3961 cccgggccac accaacacat cggcgttgtc ccggcactcc tccagcagca gagga
//
LOCUS       DAOSPL010000223         4012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22579_length_4012_cov_3.336113, whole genome shotgun sequence.
ACCESSION   DAOSPL010000223 DAOSPL010000000
VERSION     DAOSPL010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22579_length_4012_cov_3.336113"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLQ54_05485"
     CDS             complement(<1..222)
                     /locus_tag="PLQ54_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92744.1"
                     /translation="MSEAAATLVYSSRGGIVEWVGDMAGNRWTADSIIAMIRKLAAAR
                     VDLSPTGVRESHSALFSAARSKSHFGSWRA"
     gene            complement(575..3754)
                     /locus_tag="PLQ54_05490"
     CDS             complement(575..3754)
                     /locus_tag="PLQ54_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOS92745.1"
                     /translation="MWLTDVSIRRPVAMSMVFVAIAVLGFISYRDLPLELFPDTEFPM
                     VTVVTTYPGAGPDEIERLITDPIESGTASCNGLDEMTSQSIENYSVVMLQFVYGTDVD
                     AAAADVRDKVSAVKAQLPTDAEDPVVSKFSFTSTPIIRIGVAGDRPPRDLKTLVDERV
                     KDRLSAIPGVANVTVSGGEIREIQIELHQDRLKAVGLSVLDVRNQLVAQNLDLPGGSV
                     DEGRREYSVRLLGEFKTVDEIRDVWVTTGTGARVRLTSLATIKDTIADVETTSRVNGR
                     PSVSIGVQKATGANTVKVAEAIRETVKKLDEELPGDLSFVITRDESTFTEEALHDLTT
                     SLGLGVLMAALVVWAFLRSVPATTIVFLAIPTSMIATFGPMKMAGYSLNFMSMLGLSL
                     AVGALVDDSIVVLENIYRHLSMGKSPAQAALDGRMEIGLAAVSITMTDVVVFVPIALM
                     KGIVGQIFRPFGFTVAFAVLFSLFVSFTLTPMLAARWLKSGAHHGSEEEGDAAERAIR
                     KRSLYVRMLSVAIHPAWRWPVALGTLVVLWSTFALIFPKVGKEFTPTQDQGMIDIYLE
                     LPTGVRIEETDKAVREVERLVAQLPEVESYTAVAGEGSASASGSHFGTVALKLHDRTG
                     WLGRLRNKPGTIIEGKDGKKQRVSGVFETVEKLRQMVRDVPGADVRVELGGGRGPGGA
                     DVQVQLIGRQEDGLIQVHDRVLAAMQTVPELSGVSTSWKPGKPEVQAKIDRKRAGDLG
                     FTAAELAQAIRVAVEGDTTTEYREAGVEYDVRIRLREQDRLNPQDIGGILVGVRNGRP
                     IRLEDVADIRFGRGPVRIDHKNRQQYVSVSGNFALDTATGKPIPSNVAEAAAKKVVEA
                     VALPPGIKYEMGGEAEFRTEAFGNMGEAMILAVILVFLLLSALYESLFYPAIIMTVVP
                     MAMVGGLMVLWLTGLRFGIVSIIGFIMLVGMVSKNSILLVDYTNTMRSRGMSRADALL
                     RSGPTRFRPIMMTTLSLVFALAPIASGLGKGAELRQPLAATVVGGMIVSTMLSLLVVP
                     AFYSLVDDFQVYVYGRLKRFLFRLPVDEV"
     gene            complement(3791..>4012)
                     /locus_tag="PLQ54_05495"
     CDS             complement(3791..>4012)
                     /locus_tag="PLQ54_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92746.1"
                     /translation="VVVLQGVNAGDEVVVSGQASLSDGTPIKRADLSASSSPAAPGEA
                     TPSAEAPGGAAEAASAGGPGAPKAKEEGR"
BASE COUNT          681 a         1402 c         1309 g          620 t
ORIGIN      
        1 cgcgcgccag ctcccgaagt gactcttcga gcgggcggca ctgaacaggg cgctgtggga
       61 ctcgcggact ccagtggggg acaggtcaac ccgggcggcg gcaagcttac gaatcatcgc
      121 gataatgcta tcggcggtcc accgattgcc tgccatgtcg ccgacccact ccactatgcc
      181 gccccgggac gaatagacca gagtggccgc cgcctccgac acgatcctcc tcacgagggc
      241 gagagttacc atgccatgcc caagcagcgg ctaagtcgtc tattatccct aactggctag
      301 ttaccccgtc taggctccag ggggcgcgtc tcgctccgcc agaagacgac ttcgccgcag
      361 gcgctcacgc acgcagctct gccagggccg cgaaccccac cgcccagcgg cacacgcctc
      421 gacgccgctg agggtgccga accggaacca acgcgccgca cgttctttcg aacgccgccg
      481 ggccgccacc ttcatggacg ccaactgctt tcgacttcct ccgtccgccg cccgcgtgga
      541 tcgacccatg gcgcccctcg gcgacgcccg cgagctagac ctcgtccacg ggcaggcgga
      601 acaagaagcg cttcagccga ccgtagacgt agacctggaa gtcatccacc agcgagtaga
      661 aggccgggac gaccagcaac gagagcatgg tcgagacgat catcccgccc accaccgtgg
      721 ccgccagggg ctggcgtagc tcggcgccct tgcccagccc cgaggcgatg ggcgctagcg
      781 cgaacaccag gctcagggtg gtcatcatga tgggccggaa acgcgtcggg cccgaacgga
      841 ggagcgcgtc cgcccggctc atcccccggc tcctcatggt gttggtgtag tccaccagca
      901 ggatgctgtt cttcgagacc atgccgacca gcatgatgaa cccgatgatc gacacgatgc
      961 cgaagcgcag ccccgtgagc cacaggacca tcaacccgcc caccatggcc atggggacga
     1021 ccgtcatgat gatggccggg tagaacagcg actcatacag ggccgacagc aggaggaaga
     1081 cgaggatgac cgccaggatc atggcctcgc ccatattgcc gaaggcctcg gtgcggaact
     1141 cggcctcgcc gcccatctcg tacttgatgc ccgggggcag tgcgacggcc tcgacgacct
     1201 tcttcgccgc cgcctcggcg acgttggagg ggatgggttt gcccgtcgcg gtgtccaggg
     1261 cgaagttgcc cgagaccgag acgtactgct gccggttctt gtggtcgatg cgcacgggcc
     1321 ctcggccgaa ccggatgtcg gccacgtctt ccagccggat gggacgcccg ttgcgcacgc
     1381 ccacgaggat gcccccgatg tcctgcgggt tgaggcgatc ctgctcgcga agccggatgc
     1441 ggacgtcgta ctcgaccccg gcctcacggt actcggtggt cgtatcgccc tcgacggcca
     1501 cgcggatggc ctgggcaagc tcggcagcgg tgaagcccag atcgcccgcg cgcttccggt
     1561 cgatcttggc ctgcacttcg ggcttgccgg gcttccacga cgtggacaca ccggagagct
     1621 cgggcaccgt ctgcatggcg gcgagcaccc ggtcgtgcac ctggatgagc ccgtcctcct
     1681 gccggccgat gagctgcacc tgcacgtcag cgccacccgg gccacgaccg ccgcccagct
     1741 cgacgcgcac gtcggcgccg ggcacatcgc ggaccatctg gcgcagcttc tcgacggtct
     1801 cgaacacgcc cgagacccgc tgcttcttgc catccttgcc ctcgatgatg gtgcccggct
     1861 tgttccggag acggccgagc cagcccgtcc ggtcgtgcag cttcagcgcc acggttccaa
     1921 agtgcgaccc cgaggccgac gcggacccct cgcccgccac ggcggtgtag gactcgacct
     1981 cgggcagctg ggccaccagg cgctcgacct cgcggacggc cttgtcggtc tcctcgatcc
     2041 tcacaccggt cgggagttcg aggtagatgt cgatcatgcc ctggtcctgc gtgggggtga
     2101 actccttacc cactttcggg aagatcagcg cgaaggtgct ccacagaacc accagggtgc
     2161 ccagggccac cggccatcgc caggcgggat ggatggcaac ggagagcatg cgcacgtaca
     2221 gcgatcgctt gcggatggcc cgctcggcgg cgtcgccctc ctcctcactg ccgtggtgag
     2281 ctcccgactt cagccaccgc gcagccagca tgggcgtcag cgtgaaggag acaaacaggg
     2341 agaagagcac ggcgaaggcc accgtgaagc cgaaggggcg gaagatctgg cccacgatgc
     2401 ccttcatgag ggcgatgggc acgaacacca ccacatcggt catggtgatc gagaccgccg
     2461 ccaggccgat ctccatccgg ccatccagcg cggcctgggc gggcgacttc cccatggaca
     2521 ggtgccggta gatgttctcc agcaccacga tggagtcgtc gaccagcgcg cccaccgcca
     2581 gcgagaggcc cagcatggac atgaagttga gggagtaacc cgccatcttc atgggcccga
     2641 aggtggcgat catcgaggtg gggatcgcca ggaacacgat ggtcgtggcg ggcaccgagc
     2701 gtaggaatgc ccagaccacc agggcggcca tgaggacgcc gagacccaag ctggtggtca
     2761 ggtcgtgcag cgcttcctcg gtgaacgtgg actcgtcgcg ggtgatgacg aaggacaagt
     2821 cacccggcag ttcctcgtcc agcttcttga cggtctcgcg gatggcctcg gccaccttca
     2881 ccgtgttggc gccggtcgcc ttctggaccc cgatgctcac cgaaggcctg ccattgacac
     2941 gggacgtggt ctccacatcg gcgatggtgt ccttgatggt cgccaggctg gtgagccgta
     3001 cccgcgcccc ggtgcccgtg gtgacccaga cgtcgcggat ctcatccacg gtcttgaact
     3061 cacccagcag ccgcacgctg tactcgcggc ggccctcatc cacgctcccg cccggcaggt
     3121 ccaggttctg ggccacgagc tggttgcgca catcgaggac cgacagcccc acggccttga
     3181 gccggtcctg gtggagttcg atctggatct cgcggatctc gccgccgctg acggtgacat
     3241 tggccacgcc ggggatcgcc gacagacggt ccttcacgcg ttcgtcgacc aacgtcttga
     3301 gatcacgcgg tggccggtcc cccgcgacgc cgatgcggat gatgggcgtg ctggtgaagc
     3361 tgaacttcga cacgaccgga tcctcggcgt cggtggggag ctgggccttg acggccgaga
     3421 ccttgtcacg cacatcggcg gccgccgcgt ccacgtcggt gccgtagacg aactgcagca
     3481 tgaccaccga gtagttctcg atggactgcg aggtcatctc gtcgagcccg ttacacgaag
     3541 cggtcccgga ctcgatggga tccgtgatca gccgctcgat ctcatcgggt ccggcgcccg
     3601 ggtaggtggt caccacggtg accatgggga actcggtgtc cgggaacagc tcgagcggca
     3661 ggtcgcggta ggagatgaag cccagcacgg cgatggccac gaagaccatc gacatggcca
     3721 cagggcgtcg gatcgagaca tcagtgagcc acatagtgag tgcgagcggc cggcagccct
     3781 cgggccctgc ctagcgccct tcctccttcg ccttgggggc gcccggccca ccggcgctgg
     3841 cggcctcggc cgcgccgccg ggcgcctcgg cgctcggcgt tgcctctccc ggggccgcgg
     3901 ggctactcga ggcgctcaag tccgcccgct tgatcggcgt cccgtccgag agcgaggcct
     3961 ggcccgagac gaccacctcg tcgcccgcgt tcacgccttg gagcaccacg ac
//
LOCUS       DAOSPL010000224         4005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22631_length_4005_cov_2.876203, whole genome shotgun sequence.
ACCESSION   DAOSPL010000224 DAOSPL010000000
VERSION     DAOSPL010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22631_length_4005_cov_2.876203"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PLQ54_05500"
     CDS             <1..535
                     /locus_tag="PLQ54_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92747.1"
                     /translation="ADRAAGANRLAQVAESTRRLVRGLANWARLRLVTLLFVLPSLWV
                     LLYSSRSARRLLGRRRRRGSGDPRGAAAALYDCLCSAVGSAVGVPRHSSTASGEYWEL
                     VRAPLAGISDNVAREAHGLFEALTAHLYGPIPPNGQELRRLSARLAAVITQLRQAAPP
                     PPVAVRLRRALVRLWGS"
     gene            complement(532..1314)
                     /locus_tag="PLQ54_05505"
     CDS             complement(532..1314)
                     /locus_tag="PLQ54_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOS92748.1"
                     /translation="MTAGAAAVAALKGGKVLVGASKSGALLKIVPVLLKTGGSMALSL
                     GFYAPMFGFWFAVGLLGLLLVHELGHALVLRALGVPFSAPLFIPFMGAVIGMKELPRD
                     ATKEAATAFGGPVLGTIGAAVCLVIYQVTGHDLMLGLATWGFFLNLFNLIPFTPMDGG
                     RIIGAVSPKIWLVVLPALLVLALLRGSPLLVLVAFMGIPRAIAGWRTPPELQDYFRVP
                     PRVRILCLIGYVALALALAALFFHCVALAYAEVHPHVGGWEA"
     gene            1795..3075
                     /locus_tag="PLQ54_05510"
     CDS             1795..3075
                     /locus_tag="PLQ54_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS92749.1"
                     /translation="MSRHRSMTRRTFLARSAALAAIPYVVPSGARGADGSVAPSERIT
                     MGAIGIGGRGTYDLQWFMRNPDVRFVAMCDARRDRREAAKALVDQAYGNTDCATYIDP
                     REMLARDDIDAVLIATSDRWHAMASIMAMRAGKDVFSEKPCAMNVGESQALAETAREF
                     GRIFQAGTQRRSEPNFVFIVQLARLGYLGKLKTLTAHIVHGFAPHVWLPAQPEPPKDE
                     VDYDLYLGPVPWHPYNRGYVDSRHAHADLWAGGLHEWGAHTFDLCQFANDTAHTGPVE
                     FVYPNNDTSEGLTARYANGVELVMTASGFPGTCGVRFTGEDGVTECSDGHDPTATPAS
                     LLGERDRILRDYVAKTRRSLDHVRDFLDCVKSGRQPIANADVARKAHNICHVGAICMA
                     LKRDLRWDPDREEFIGDDEANRMRFRANRAPWVL"
     gene            3100..>4005
                     /locus_tag="PLQ54_05515"
     CDS             3100..>4005
                     /locus_tag="PLQ54_05515"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOS92750.1"
                     /translation="MKNRDNRFGVLAALVALLAGTFASYAQPAQNVTAEEEALVGTLT
                     SGAEPFEKDKACRRLAVIGTARAVPALAALLADDQLTGMARHALLNIPDAAAGEALCA
                     ALPKLTGTKLIGVISTIGRRGESSAVKALAPFIASPDDEVAATAARAIGRIGGGDSRR
                     VLESALAIAPRSRRAAVAEGCIEGIRALTDRGATKDALALADTVRRSRVPAYLAAAAT
                     RAAVLLRGEAGALVLARQLDSPEPELFQMALLLTREAPGAAITRAVSERLGRLPVERQ
                     APLLAALGDRGDAGALPVVLAAVDST"
BASE COUNT          635 a         1332 c         1465 g          573 t
ORIGIN      
        1 ggccgaccgg gcggcgggag cgaaccggct ggcgcaggtg gcggagagta cgcgccgtct
       61 ggtccggggt ctcgcgaact gggcacgact ccggctggtc acgctgctgt tcgtgctgcc
      121 ctctctgtgg gtgctgctgt actcgtctcg gtccgcgagg cggctccttg gccgtcggcg
      181 gaggcgcggg tccggcgatc cgcgtggagc ggctgccgcg ctctacgact gcctgtgctc
      241 cgcagtgggc tctgccgtgg gggtcccgcg gcactcgtcg acggcatcgg gtgagtactg
      301 ggagctggtc cgggcccccc tggccggtat ctccgacaat gtagcccggg aggcccacgg
      361 cctcttcgag gcccttacgg ctcacctgta cggcccgatc ccacccaatg gccaggagct
      421 cagacgccta tcggcgcgtc tggccgcggt catcacgcag cttcggcaag cggcgccgcc
      481 cccgcctgtt gcggtccggc tgaggcgggc gttggtccgg ctgtggggct cctaggcttc
      541 ccagcccccg acgtgcggat ggacctcggc gtaggcgagg gccacacagt ggaagaacag
      601 cgccgccaac gccagcgcca gcgccacgta cccgatgagg cacaggatcc gcacgcgcgg
      661 cggcacgcgg aagtagtcct gcagctccgg cggggtccgc cagccggcga tggctcgcgg
      721 gatccccatg aacgcgacga gtacgagcag gggcgagcca cggagcaggg cgaggacgag
      781 gagggccggc agcacgacca gccagatctt gggcgagacc gcgccgataa tgcggccgcc
      841 gtccatgggc gtgaacggga tcaggttgaa caggttcagg aagaagcccc acgtggcgag
      901 gcccagcatg aggtcgtgcc cggtgacctg gtagatgacc aggcacacgg cagcgccgat
      961 ggtgcccagg accgggccac cgaaggcggt ggcggcctcc ttggtcgcgt ctcgaggcag
     1021 ctccttcatc ccgatgacgg cgcccatgaa tgggatgaac agcggggcgc tgaacgggac
     1081 ccccagggcg cgcagcacca gggcgtggcc cagctcgtga accaggagaa gccctaggag
     1141 cccgacggcg aaccagaaac cgaacatcgg tgcatagaac ccgagcgaca gcgccatgga
     1201 gccgcccgtc ttgagcagca cgggcacgat cttgagcaga gctccggact tcgacgcccc
     1261 gaccaggacc ttgcccccct tgagtgccgc gaccgcagcg gcaccggccg tcacgctcct
     1321 gccgtggccg cgggactggc ccgccgggcg gggttccgcg cccggggccg gcgcgggaag
     1381 gggtggtggg gtgggggcgg ggagcgtggc atcggcctcg acgcaggcag gcaccggccg
     1441 cccgcagtag gcgcagtgcc ggtcctgcgg ctccacgagg tagccacact cggggcaggt
     1501 cgcaggggtg cgggctccgg tgtccatgcc gtcagggtag cactcccgag gcactcaggg
     1561 aactggtgtc gttgtgggag gcgagcaggg caggggattc ccggatgcac tcggcgggac
     1621 gggccacgcg tgggaaatgt atgctatcgg agcgtgggtg ccggcgcggg gccactggct
     1681 acgcccacac gggaggcaag ggccgcggcg accggccggc gaaggagtgt ccaggcccgg
     1741 cttccgcaca tattccccaa tcgggccccg tttgtcacgg aggaggcaga ggccgtgagt
     1801 cgacatcgca gcatgacccg ccgcacattc ctggccagat cggcggcact ggcggccata
     1861 ccctatgtcg taccatcggg ggctcgaggg gcggatggca gtgttgcgcc cagcgagcgg
     1921 atcaccatgg gggccatcgg catcggcggc cgaggcacgt acgacctaca gtggttcatg
     1981 cgtaacccgg atgtgcggtt cgttgcgatg tgtgatgcgc gacgcgaccg ccgggaggcg
     2041 gccaaagccc tggtagacca ggcctatggc aacacggact gcgccaccta tattgacccg
     2101 cgcgagatgc tcgcgcggga tgacatcgat gccgtgctca tcgccacgag cgaccgctgg
     2161 catgcgatgg cgtcgatcat ggcgatgcgg gcgggcaagg acgttttcag tgagaagcct
     2221 tgcgccatga acgtgggcga gagccaagcc ctggccgaga ccgcgcggga gttcggccgg
     2281 atcttccagg ccggcacgca acgacgcagc gagccgaact ttgtgttcat cgtccagctg
     2341 gcccgcctgg ggtacctcgg caagctcaag acgctcaccg cccatatcgt gcacggcttc
     2401 gcaccgcacg tatggctgcc cgcccagccc gagccgccga aggacgaggt ggactacgac
     2461 ctgtacctgg gcccggtgcc ctggcacccc tacaaccggg gttacgtcga cagccgccac
     2521 gcgcacgcgg acctgtgggc cggcggcctg catgagtggg gtgcccacac cttcgacctg
     2581 tgccagttcg ccaatgacac ggcgcacacg gggcccgtcg agttcgtcta cccgaataac
     2641 gacaccagcg agggcctgac ggcgcggtat gccaacgggg tggagctggt catgaccgca
     2701 tcgggcttcc cgggaacctg cggtgttcgc ttcacgggcg aagacggtgt cacggagtgc
     2761 tccgacggcc acgatccgac ggcgacgccg gcgtcgctgc tgggcgaacg cgaccgcatc
     2821 cttcgggatt acgtcgccaa gacgcgccgc tcgctggatc acgtgcgtga cttcctcgac
     2881 tgcgtcaagt cgggccgcca gccgatcgcc aacgccgacg tggcccggaa ggctcacaac
     2941 atctgtcacg tcggagcgat ctgcatggcc ctgaagcgcg atctgaggtg ggacccggat
     3001 cgcgaggagt tcatcggcga cgacgaggcg aaccggatgc gcttccgcgc caaccgcgcc
     3061 ccctgggtgc tctaggtgct gtttgaggga gaagaggaca tgaagaaccg cgacaatcga
     3121 ttcggtgtgc tggctgcgct tgtggcgctg ctggcaggca ccttcgcatc ctacgcccaa
     3181 ccggcgcaga acgtgacggc cgaggaggag gcactggttg gcacccttac gtccggtgcg
     3241 gagccgttcg agaaggacaa ggcatgccgc cgcctggctg tgatcggcac ggcgagggcc
     3301 gtgcccgcgc tggccgcgtt gcttgccgat gatcagctca cgggaatggc gcgacacgca
     3361 ctgctgaaca tccccgacgc cgcggcggga gaagctctgt gtgcggccct gccgaagctg
     3421 acgggaacga agctgatcgg tgtgatcagc accatcggcc ggcggggtga gagctcggcc
     3481 gtgaaggcgc tggcgccgtt catcgccagc ccagacgacg aagtcgccgc cacggcggcg
     3541 agggccatcg gccgcatcgg cggtggcgat agccggcggg tcctcgaatc cgccctggcc
     3601 atagcgccgc gctcgcgccg ggcggccgtg gccgaggggt gcattgaggg cattcgagca
     3661 ctgacggacc ggggcgccac gaaggatgca ctcgctctcg ccgacacggt ccggcgttcc
     3721 cgggtgcccg cgtacctggc ggccgccgcc acgcgggcag ccgtgctcct gcgcggcgag
     3781 gccggggccc tcgtgctggc tcggcagctc gacagcccgg agcccgagtt gttccagatg
     3841 gcgctgctcc tgactcggga ggccccgggc gcggcgatca cccgtgccgt ctccgagaga
     3901 ctgggccgcc ttcctgtgga gcgccaggcc cctctgctgg ccgcgttggg tgaccgagga
     3961 gatgccgggg ctcttcccgt cgtactggct gcggtcgaca gcacg
//
LOCUS       DAOSPL010000225         3998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22682_length_3998_cov_3.204413, whole genome shotgun sequence.
ACCESSION   DAOSPL010000225 DAOSPL010000000
VERSION     DAOSPL010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22682_length_3998_cov_3.204413"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2104)
                     /locus_tag="PLQ54_05520"
     CDS             complement(<1..2104)
                     /locus_tag="PLQ54_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOS92751.1"
                     /translation="MARKGARSVSAPANKLVYETAGIVCLTLGAILLVALSSGSSGAV
                     TALLIRGLRCGFGVGAYAVPLGVMLAGVLLIVRYEEALRVRPAGAGLPLLCAVILTFA
                     HLPADPATRFDEEVLVSGGGVVGATLAAVLSVCFGATGAYIIAAGGALAAVAIASPKP
                     LAVLLGPAAVAAQRLWARLRAFWARKSEERRRMRVARQAKAEPRARRVKAPRPPKPAV
                     LDEPVAQCSPPAPAPASASPEPPVPAAPAEPPIPAWLIPPREGPALPGAPTPAQHPAS
                     PVDWSDEQGTLDLPLRENRPSRPTKPYELPTLDLLDDTPPFDQERHAKEAAETIRLLE
                     EALADYSIGARVVSIERGPSVTRYEVQPDKGVRGASVTKLADDLARALAAIDVRVESH
                     VPGKSVIGIEVPNRTVAIVSLKEIMESQEMQASKSPLSFALGRDISGNCVVGDLTRMP
                     HLLVAGATNSGKSVCLNGLILSILMRTKPHEVKFMMIDPKRVELALYDNIPHLISPVV
                     YDAKEAAGLLRQAIAEMEERYRLLASAGVRNIGEFNKRAERLGEEKLFYLVIVVDELA
                     DLMMQAAAEFEYSICRIAQLARAVGIHLIVATQRPSVNVVTGLIKANIPSRVAFAVSS
                     QTDSRVILDCVGAERLIGRGDMLYSPIDASKPRRIQGAYTSVEEINRVVDHLQRQGDP
                     EFLIEPVPIDDEDPSGGTP"
     gene            2324..2620
                     /locus_tag="PLQ54_05525"
     CDS             2324..2620
                     /locus_tag="PLQ54_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOS92752.1"
                     /translation="MPTYVYQCRKCGEQFERVQRITDAPLSKCESNGCRGSVFRVIQP
                     VGIQFKGSGFHINDYGRGSRRNGGNGSTKGKDDVKSDAKCESKCDGKCPAGASA"
     gene            2908..>3998
                     /locus_tag="PLQ54_05530"
     CDS             2908..>3998
                     /locus_tag="PLQ54_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92753.1"
                     /translation="MPRPWVLVCLLCLSSVCVAAAEVTIDDFESLDAWAVHADGGRGA
                     DFSADAAVVHQGDAAMRVRYAAAPPQWCNLVRPVTVPGPATEVALWVYVRTAGPKAGM
                     HLWFMEPDGDGWVAPVLADGERVGQWGPGWHDVRVPMGSFQFQPRGAKTRQLTRADRM
                     LLGFNFDDMDVTIDSLRFVLRDAEGGEAMPPRTAGLVIARGSRGSAVILKDTLPTTPG
                     AADPVRIGEALAEASWGVTYITAADLCDAAVLDPSRVDLLVLPGGPAYPARCADALRA
                     YLKAGGALIAIGGYALDRPMSFVEGAWRPVGVGLTAEQVTGAADEASRPLNTRMGQPG
                     DALVLDPEQVQVFDPQFVLKGTTAIRAAEG"
BASE COUNT          624 a         1322 c         1376 g          676 t
ORIGIN      
        1 cgggcgtgcc gcccgaggga tcctcgtcgt cgatggggac cggctcgatc aggaactcgg
       61 ggtcgccctg cctctggaga tggtcgacca cccgattgat ctcctcgacc gaggtgtagg
      121 cgccctggat gcgccgcggc ttgctggcgt cgatgggtga gtacagcatg tcgccgcggc
      181 cgatcaggcg ttcggcgccg acgcagtcca ggatgacccg cgagtcggtc tgggatgaca
      241 cggcgaaggc cacacgcgag ggaatgttgg ccttgatcag gcccgtcacc acgtttaccg
      301 acggccgttg cgtggccacg atcaggtgga tgcccaccgc gcgcgcgagc tgggcgatgc
      361 ggcagatgga gtactcgaac tcggccgctg cctgcatcat caggtccgcc aactcgtcga
      421 ccacgatgac caggtagaag agcttctcct cccccagccg ctcggcgcgc ttgttgaact
      481 cgccgatgtt ccgcacgccc gccgacgcca ggagccggta ccgctcctcc atctcggcga
      541 tggcctgacg gagcagtccg gcggcctcct tggcgtcgta gaccaccggc gagatcaggt
      601 gcggaatgtt gtcgtagagc gcgagttcga cccgctttgg gtcgatcatc atgaacttga
      661 cctcgtgggg tttggtccgc atcaggatcg acaggatcag cccattgagg cacacgctct
      721 tgcccgagtt ggtggcgccc gccacgagga ggtggggcat gcgggtgagg tcacccacga
      781 cgcagttgcc cgagatgtct cggcccagtg cgaagctcaa gggcgacttc gaggcctgca
      841 tctcctggct ttccatgatc tccttgaggc tgacgatggc gacggtccgg ttgggtacct
      901 cgatgccgat gactgacttc ccgggtacgt gtgattccac gcgcacgtcg atggcggcca
      961 gcgctcgcgc cagatcgtcg gccagcttgg tgacggacgc gccccgcaca cccttgtcgg
     1021 gctgcacctc gtaccgcgtc accgaggggc cgcgctcgat ggaaaccacc cgggcgccga
     1081 tgctgtagtc ggccagtgcc tcttccagca gacggatggt ctcggccgct tccttggcgt
     1141 ggcgctcctg gtcaaacggt ggcgtgtcgt ccaggagatc gagggtgggc aactcgtagg
     1201 gctttgtggg ccgtgacgga cggttctccc ggagcgggag atccaaggtg ccttgctcgt
     1261 cactccagtc caccggactg gcagggtgct gggcaggggt tggcgctccc ggcaatgcgg
     1321 ggccctctcg tggcggaatg agccaggcgg gtatgggggg ctcggccggt gccgccggca
     1381 ccggcggctc gggcgacgcc gaggctggag caggggccgg tggagagcac tgggcgacgg
     1441 gctcatcgag gactgcgggc ttcggcggcc ggggcgcctt gacgcgacgc gctctgggct
     1501 cggccttggc ctggcgggcg acgcgcatcc gccggcgctc ctcggacttc cgtgcccaga
     1561 aggcgcgcag gcgtgcccag agacgctggg ccgcgaccgc ggcggggccg agaagaaccg
     1621 ccagcggctt cggagacgca atggccaccg ccgccagggc ccctcccgcc gcaatgatgt
     1681 aagcccccgt ggcaccaaag cacaccgaca ggaccgcggc cagcgtcgct cccaccacgc
     1741 cgcccccgga caccagaacc tcctcgtcga accgcgtcgc cgggtcggcg ggaaggtgag
     1801 cgaaagtcag aatcaccgca cagaggagcg gcagacccgc tcccgccggc cggacccgca
     1861 gcgcctcctc ataccgaacg atcagcagaa cccccgccag catgacaccc aacgggaccg
     1921 cgtaggcacc cacgccgaag ccgcagcgca ggcccctaat cagcagggcc gtcaccgcgc
     1981 ccgatgaccc tgagctcaac gcaaccagca gaatggcgcc gagcgtcagg cacacgatgc
     2041 cagccgtctc gtagacgagt ttgttggcgg gtgccgagac gctccgggca cccttacgag
     2101 ccacgtgact ccacctcccc acgctcaacc ccgcccattg gccccgtggt tcaagagccg
     2161 tccggcccag gctatgagca tggtgctatc aatgcccttg ctattagcaa catagggttg
     2221 tagactgcta gcggtacggt agaatgcgca gtggcatgac gcatcaccgt gtggcggttc
     2281 ggcgtgggcc acgcgtgcta ttgagctagg aggggctagg gtaatgccga cctacgtcta
     2341 ccagtgtcgg aagtgtgggg agcagttcga gcgtgtccag cggatcacgg atgccccctt
     2401 gtccaagtgt gagtccaacg gttgccgggg gtcggtgttc cgcgtcatac agcccgtcgg
     2461 tatccagttc aagggctccg gattccatat caatgactat ggccgcggca gtcgcaggaa
     2521 cggcggtaac ggctccacca aaggcaagga cgacgtgaag tccgacgcca agtgcgagtc
     2581 caagtgtgac ggcaagtgcc cggctggcgc ctcagcgtaa tgcccgacgt tgcggcatca
     2641 tagacggatc gcacgccgag ggagccctgg ccggtcgggg tcggggctct ctcgctcggt
     2701 gttggtcgac ccctcgttgt cttggtcgcg gcggggcacc catgcacgcc gcgggacctc
     2761 ccggtaaccc gtgtggcgcg ctccccggcc aggctgcacc cgtctcggcc ccgaaggccg
     2821 agcgatgcgc cgcggcgaag ggccgtgtca cctgtgtgcg tcgcttggtg tgaactggcg
     2881 cggtatgcag gcgtgggagg tttggccatg ccgagaccgt gggtcctggt ctgtcttctc
     2941 tgcctatcct ctgtgtgtgt tgctgccgcc gaggtcacta tcgacgactt cgagtcgctc
     3001 gacgcctggg ccgtccatgc cgatggcggc cgaggggccg acttctcggc cgatgccgcc
     3061 gtggtccatc agggcgacgc ggccatgagg gtgcgctatg ccgcggcgcc tccccagtgg
     3121 tgcaatctag ttcgcccggt taccgtgccc ggcccggcca cggaagtggc actatgggtc
     3181 tatgtccgca ctgcggggcc caaggccggg atgcacctgt ggttcatgga gcccgacggc
     3241 gatggctggg tcgcccccgt cttggccgat ggtgagcgag ttggtcagtg ggggcccggt
     3301 tggcacgatg tacgtgttcc tatgggctcc tttcagttcc agccgcgcgg cgccaagacc
     3361 agacagctca cccgggctga ccggatgctt cttgggttca acttcgacga catggacgtc
     3421 accatcgact cgctgcgctt cgtgctacgc gacgccgagg gaggagaggc catgccgccg
     3481 cgaaccgccg gcctggtgat tgcccggggc tcccgggggt ccgccgtgat tctcaaggat
     3541 acactaccca ccacacctgg agctgcggac cccgtccgca tcggtgaggc gctggccgaa
     3601 gcgagttggg gcgtgaccta catcaccgcc gcagacctgt gtgacgcggc ggtcctcgac
     3661 ccgtcgcgag tcgatctgct ggtgctgccc ggaggtcccg cttacccggc acggtgtgcc
     3721 gatgcactcc gtgcctacct caaggccggc ggggccctga tcgccattgg cggctatgca
     3781 ctggaccggc ccatgagctt cgtggaaggc gcctggcgtc ctgtgggggt cgggttgacc
     3841 gcggaacagg tcaccggtgc ggccgatgaa gcctctcggc ccctcaacac ccggatgggc
     3901 cagcctgggg atgcgctggt tctcgacccc gagcaggttc aggtcttcga tccacagttc
     3961 gtgctcaaag ggaccaccgc catccgggct gccgaggg
//
LOCUS       DAOSPL010000226         3997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22687_length_3997_cov_2.968290, whole genome shotgun sequence.
ACCESSION   DAOSPL010000226 DAOSPL010000000
VERSION     DAOSPL010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22687_length_3997_cov_2.968290"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLQ54_05535"
     CDS             <1..343
                     /locus_tag="PLQ54_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92754.1"
                     /translation="TVEGDPVVLADLKPAQSGTVGVRVRLTTPGTADLVPTSFEVTAD
                     EIEQPLTVMVSLPCTDKGIQLASMEQAAPVGEMGVRVLRFGDYYLLLSALPLAAVLGA
                     LACRWRRQSHP"
     gene            276..980
                     /locus_tag="PLQ54_05540"
     CDS             276..980
                     /locus_tag="PLQ54_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOS92755.1"
                     /translation="MPCRWRPFSARWPVDGGGSRIPSGPDPNASLGALQGAALGLSMA
                     LGCLSSCLPVLAPYMATHTDRPRDGVGAALVFSGGRLLGYCLVLGTALALGARLPDAS
                     WVRGASAVSLLVLGGLMLAYAVHANIPGARFCKLRGFTELLRRFPAAAGMLVGLSPCP
                     PLLTAAATLAGGHAGQASAFIAAFAVGTAAVTLPLAWLGHPKAPRVVASIARFAAVFI
                     GMWFVARAAALLGSAR"
     gene            985..2358
                     /locus_tag="PLQ54_05545"
     CDS             985..2358
                     /locus_tag="PLQ54_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92756.1"
                     /translation="MSNCRCWALRTTAVVMLAVCLATSVCCRRSMAADPGPLAARGAA
                     AAVRGDGQDMPFPEALSPTQVRQLSASKVGELGVAWIGADAQARGFSAQGECLGSEVE
                     LAAWRYRTAEAQEKAWVKSVGDLVGEEVGPGGTWAESFVDGRAVCAARFERDGQPWVA
                     VAASANQPAAQEAACLMRSAAELVYPKTAGDWSLPEPAKGYDRETVYDPIDGAADQYI
                     RYHFVRFYRGRYQKGDRTVLADVYQQATSADAYGVQSAFQGKEPAYVGGWGFSTGPQV
                     TFWQGPYFCRIMAEWMGEGAGDAALEVAEAISHTIGVNGPEPSMLNVFADLDPQPARR
                     YYFHMHKDLIPLFYLSTNNLLGLDADTDCVLAKLPPDKEGPVALAVRYPDRARAEAGL
                     AGLLKVVLPDVAPTEGMRVGVWDQGRWAGAATKESVPGAWVVAAFDAVDEAQCRAMVQ
                     SLLERVP"
     gene            2355..3353
                     /locus_tag="PLQ54_05550"
     CDS             2355..3353
                     /locus_tag="PLQ54_05550"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOS92757.1"
                     /translation="MKPRKVTLVRRLVQLAFFILLLYGAFIFPHPLEAPIPSIEPGTP
                     RTSQYPRDRILWVSGKECVIDMYLPVLACRFIAQGGTFKSCSLHVLSENITWKTSIKL
                     LLPHLAFLAVLSFLFARMWCGWACPLGAMQDAMGWIRRQFGAGPWHLAQGWDRFFRVL
                     RHVLLWLSLAISALITLPLFGRTGVNDALFLIYCQLCPSRLVYPPLGGVNPCWTDFTN
                     GITTTLTFLGWFCLGVFALGFMVPRLWCRFCAVGALLSYFNRGGLVTLEKQAGKCTSC
                     GTCRRVCPVEVQRVYRDRTNPIVTDTQCTLCGRCVESCPEEGCLSMRLAGRSIVES"
     gene            3357..>3997
                     /locus_tag="PLQ54_05555"
     CDS             3357..>3997
                     /locus_tag="PLQ54_05555"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOS92758.1"
                     /translation="MPALDYFREVLETAQRAEELKTGSRPVVGTMCNFVPEALIIAAG
                     AVPVRICAGDHSAALEGEALVPRDICPVCKSTVGTLQRPGGPHGRLDLLVVPAVCDAK
                     KKLPQVLEGQAPVHVMELPVDKQGQGAERRWLDQVRLLAERLEKLTGQRITRQGLRGA
                     IDLINARQNAYRELLALRQENPPRLSGLEALLLANASFTDDVARWTHHARALAS"
BASE COUNT          537 a         1302 c         1384 g          774 t
ORIGIN      
        1 caccgttgag ggcgacccgg tggttctggc cgacctgaag ccggcgcagt cgggcaccgt
       61 gggtgtgcgc gtccggttga ccacgccggg caccgccgac ctggtaccga cctcgttcga
      121 agtgaccgct gacgagattg aacagcctct gacggttatg gtctcgctgc cgtgcactga
      181 caagggcatc cagttggcct cgatggagca agcagcgccc gtgggtgaga tgggtgtgcg
      241 cgtgctgcgg tttggtgact attatctgct gttgagtgcc ttgccgctgg cggccgttct
      301 cggcgcgctg gcctgtcgat ggcggaggca gtcgcatccc tagcggcccg gatcccaacg
      361 catcgctcgg ggccctgcag ggagcggcac tgggcctgtc gatggctctg ggatgtctca
      421 gttcgtgtct cccagttctg gcaccctaca tggcgaccca cacggaccgg ccaagggacg
      481 gcgtcggcgc cgcgctggtg ttcagcggtg gcaggctcct gggctactgc ctggtgctcg
      541 gcacggcact ggcattgggg gctcgcctgc cggacgcctc gtgggttcgt ggggcctcgg
      601 ccgtatcgtt gcttgtgctc ggcggcctga tgctggccta tgcggtgcat gcgaacatcc
      661 ccggagcacg cttctgtaag ctccggggct ttacggagct tctgaggcga ttcccggctg
      721 ccgcagggat gctggttggg ctcagcccgt gcccccccct gctgactgcg gccgcgactt
      781 tggccggcgg gcatgctggc caggcctctg ctttcatcgc ggcgtttgcg gttggcacgg
      841 cggcggtgac gctgcccctg gcgtggctgg ggcatccgaa ggcgccccgg gtggtggcca
      901 gtatcgcgag gttcgccgcc gtgttcatcg gcatgtggtt cgtcgcccgc gcggcggcac
      961 tgctgggttc agcgaggtga tcgaatgagc aattgcagat gctgggcgtt gcgcacgacc
     1021 gcggtggtga tgctcgctgt ctgcctggcc acctccgtgt gctgccgccg ctccatggcg
     1081 gcggacccgg gacctctcgc cgcccgggga gctgccgcgg ctgtgcgggg cgacggacag
     1141 gacatgccgt ttcccgaagc gctcagcccg acccaggtgc gccaactcag tgcgtccaag
     1201 gtcggcgagc ttggggtggc ctggatcggc gccgacgctc aggcgcgggg attctcggct
     1261 cagggggagt gcctcgggtc cgaggtcgag ctcgctgcgt ggcggtaccg gactgccgag
     1321 gcgcaggaga aggcctgggt gaagtcagtc ggcgatctgg tgggcgagga ggtcggtcct
     1381 ggaggcactt gggctgagtc cttcgtcgac ggccgcgccg tctgcgccgc gcggttcgag
     1441 cgcgatgggc agccgtgggt ggccgtcgct gcttcggcta accagcccgc ggcgcaggag
     1501 gctgcgtgcc tgatgcggtc ggcagccgag ctggtgtacc cgaagacggc gggcgactgg
     1561 agcctcccgg agccggccaa aggctacgat agggagacag tctacgaccc catcgatggc
     1621 gccgccgatc agtacatccg ttaccatttc gtccgtttct accggggccg gtaccagaag
     1681 ggcgaccgta ccgttctcgc cgacgtgtac cagcaagcga cctcggccga cgcctacggt
     1741 gtccagagcg cgttccaggg caaggagccg gcctacgtgg gcgggtgggg attctcgacg
     1801 ggcccgcagg tgacgttctg gcaggggccc tacttctgcc ggatcatggc cgaatggatg
     1861 ggcgagggcg cgggagacgc cgcgctcgag gtcgcggaag ccatcagcca caccatcggc
     1921 gtgaacggcc ccgagccctc aatgctcaac gtcttcgccg atctcgatcc ccagcccgca
     1981 cggcggtact actttcacat gcacaaggac ttgatcccgc tgttctacct ctcaaccaac
     2041 aacctgttgg gtctggatgc cgacacggac tgcgtgctgg ccaaactgcc gccggacaag
     2101 gaagggccgg tggctctggc tgtgcggtac cccgaccgtg cgcgggccga ggcgggcttg
     2161 gcgggtctgc tgaaggtcgt tctacccgat gtggctccga ctgaaggcat gagggtgggc
     2221 gtctgggatc aggggcgttg ggccggcgcg gctaccaagg agtcggtccc gggtgcctgg
     2281 gtggtcgcgg cgttcgacgc cgtcgatgag gcccagtgcc gtgccatggt ccagagtctc
     2341 ttggagcggg tgccgtgaag ccgcgcaagg tcacgctggt ccgccgcctc gtccaactgg
     2401 cgttcttcat cttgctgctc tatggcgcat tcatcttccc tcatccgctc gaggcgccca
     2461 tcccctcgat tgagccgggc actccccgta cgtcgcagta cccgagggac cgcatccttt
     2521 gggtgtccgg taaggagtgc gtcatcgaca tgtacctgcc cgtgttggcc tgccggttca
     2581 tcgcccaggg cggcacgttc aagtcttgtt cgttgcacgt cctgtccgag aacattacct
     2641 ggaagacctc catcaagctc ctgctccctc acctggcttt cctggcggtt ctgtcattcc
     2701 tcttcgcgcg gatgtggtgc ggctgggcct gtccccttgg ggccatgcag gatgccatgg
     2761 gctggattcg ccgccagttt ggcgccgggc catggcacct ggcgcaagga tgggaccgct
     2821 tcttccgtgt gttgcggcac gtgctgctct ggctctcgct ggccatctcc gcgctgatca
     2881 ccctgccgct gttcggccgc actggcgtca acgacgcgct gttcctgatc tactgccagc
     2941 tctgcccgtc gcgcctggtg tacccacccc ttgggggtgt caatccgtgc tggaccgact
     3001 tcacgaacgg gatcaccacc accctgactt tcctcgggtg gttctgtctg ggtgtgttcg
     3061 ccctgggctt catggtgcca cggctctggt gccgcttctg cgccgtcggt gccttgctct
     3121 cgtacttcaa tcgcggcggg ctggtgacgc tcgagaagca ggccggcaag tgcacgtctt
     3181 gtggtacctg tcgtcgagtg tgtcccgtcg aagtccagcg cgtctaccgt gaccgcacca
     3241 acccgatcgt cacggacacg cagtgcacct tgtgtggccg gtgtgtggag agctgccccg
     3301 aggaaggttg cctctcgatg cgcctcgccg gccgatcgat agtggagtcg tagcccatgc
     3361 ctgcccttga ctactttcgt gaggtcctcg agaccgctca gcgcgctgaa gaactcaaga
     3421 cgggctcgcg tcccgtcgtg ggcaccatgt gcaacttcgt gcccgaggcc ctcatcatcg
     3481 cggccggcgc cgtgcccgtc cgcatttgcg cgggcgacca ttcggcggcg ctggagggcg
     3541 aggccctggt ccctcgcgac atctgccccg tgtgcaagtc cacggtggga acgctccagc
     3601 gtccgggtgg gccgcacggc cgcctggacc tgctcgttgt tcccgccgtg tgcgatgcca
     3661 agaagaagct gccgcaggta ctcgagggcc aagcccccgt gcatgtgatg gagcttcccg
     3721 tcgacaagca gggccagggg gcggagcgtc gctggctcga tcaggttcgc ctcttggccg
     3781 agcggctgga gaagctgacc gggcagcgca tcacccggca gggcttgcgt ggagctatcg
     3841 acctcatcaa cgcgcgtcag aatgcctacc gcgagctcct ggcactgcgc caggagaacc
     3901 ctccgcgcct atcgggcctc gaggcgctct tgctggccaa tgcgagcttc accgatgatg
     3961 tggcacgctg gacccaccac gcgcgtgccc tggcctc
//
LOCUS       DAOSPL010000227         3996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22693_length_3996_cov_3.080436, whole genome shotgun sequence.
ACCESSION   DAOSPL010000227 DAOSPL010000000
VERSION     DAOSPL010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22693_length_3996_cov_3.080436"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>225
                     /locus_tag="PLQ54_05560"
     CDS             <1..>225
                     /locus_tag="PLQ54_05560"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92759.1"
                     /translation="PVRRQGSEVRVWGRSYRLGALPLPAEIETANESILAGPITLEAV
                     VDGKRQSWRPDGALHVDSARPNRLVVTARA"
     assembly_gap    226..235
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <236..2519
                     /locus_tag="PLQ54_05565"
     CDS             <236..2519
                     /locus_tag="PLQ54_05565"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92760.1"
                     /translation="QCVGHVTTEYDGLLRSDLVIQPEGARQIDHLSLDVPIRPQFALY
                     LYTFPGQWGSSRNARALPEGDWASPFKPMVWIGDNDRGLEWFAESDEGFRLADANRAL
                     EIIRSADAITLRVNLVTAPLPAENLEFSFGFQATPVKPLTPDVWDYRICHHGGYGIES
                     QPWKAPATVTYRAEGHIDLSQGTFEAWVRPRFDTQPRVDPDDPARGALNRNLFDVLFA
                     DSSRIGFYWNIDDLGMRVYCRQGTNTFPVILGSRAAWQRDEWHHVAFTWGERLAIYLD
                     GALVAETAHRGLLDAPLRDARIVLGEGESEFDLDEVRISDVSRDAFDLTHAPEADAHT
                     LLLDHLALDPGATRRTSPARGEGGVATGWVVGEGRFGPALARPDEAAQMTLLDRLAQL
                     GVRTLVFHEHWTDIQNYTSTTHGDQLRSLVRACHDRGMRLLLYFGYEISDIAPEFAAY
                     ADEMLVAPRQGGYTRQPPQTAYIVCYQSKWQDFMAQGIARVMDEYDIDGVYLDGTEYP
                     WGCANTRHGCGYTRPDGTIGTTWSIYGAREMLRRIYTIVRSRKPDGQVNVHNSTCMAM
                     PSLAFATSSWDGEQLGGMEPGPDPLTVLPLDTFRCEFMGRQWGVPAEFLCYDRPYTWS
                     QALSFTLLHDVLVRGSLGGSLEAESKLWHAMDDFGRHEATWLPYWDNGRYVTLGPDAS
                     IKSSLYWRPGKGVVAVVSNLGPKASNAEVRLNTAALKLVGDLQATDILSDDPVDISRD
                     GRMSFPLPSFGFRVVWVREQ"
     gene            3237..>3996
                     /locus_tag="PLQ54_05570"
     CDS             3237..>3996
                     /locus_tag="PLQ54_05570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS92761.1"
                     /translation="MASLDILRDPAELERFAGQWPDAAQADGGAALGTSLLWIESWFA
                     AFGAEGAPEIWSFSETGRWLGAIPLVRRREPSPWRVSPRLDVLAGAANVHTPDYGIAG
                     PSPDRVLDAFCRELAARRHRWDRVILTRIPAASALATDGPRAFACHGLSWLRLPTAFQ
                     AKIPTGQGENAWAAARRSLVRKTGQYRRALERQGDLRFDMICSPPGSDTAFEQFTTLE
                     SAGWKGARGSAISSSEVTRRFYSTLVRRAAEDRSL"
BASE COUNT          622 a         1470 c         1275 g          619 t
ORIGIN      
        1 cccccgtgcg ccggcagggc agcgaggtcc gcgtttgggg ccggtcgtac cgcttgggcg
       61 cgctgcccct cccggccgaa atcgagaccg ccaacgagtc catcctcgcg ggacccatca
      121 cgctcgaagc cgtcgtcgac ggaaagcgcc agagctggcg ccctgacggc gcactccacg
      181 tcgactcggc ccggcccaac cgcctcgtag tcaccgcccg tgcggnnnnn nnnnntcagt
      241 gcgtcggcca cgtcaccacc gagtacgacg ggctgctccg gtcagacctg gtgatccagc
      301 ccgagggcgc acggcaaatc gaccacctga gcctcgacgt gcccatccgg ccccagttcg
      361 cgctctatct gtacacgttc ccgggccagt ggggcagttc acgcaacgcc cgagcgctgc
      421 ccgagggcga ctgggcctcg cccttcaagc ccatggtctg gatcggcgac aacgatcgtg
      481 ggctcgagtg gtttgccgag tccgatgagg ggttccgtct ggccgatgcc aatcgcgccc
      541 tcgagatcat ccggtcggcc gatgccatca cgctccgtgt gaacctcgtc acggcacccc
      601 tgccggccga gaacctcgag ttcagcttcg ggttccaggc tacgcccgtg aagccgctca
      661 cccccgatgt ctgggactac cgcatctgcc atcacggtgg ctacggcatc gagagccagc
      721 cgtggaaagc cccggcgacg gtcacctatc gcgccgaagg gcacatcgac ctgagccagg
      781 gcacattcga ggcatgggtc cggccccggt tcgatacgca gccccgcgtt gacccggacg
      841 accccgcccg cggcgccctg aaccgcaacc tgttcgatgt gctttttgct gacagcagcc
      901 gcatcggctt ctactggaac attgacgacc tcggcatgcg cgtctactgc cgccagggca
      961 ccaacacgtt tcccgtgatc ctcggctcac gggcggcgtg gcagcgcgac gagtggcacc
     1021 acgtcgcctt cacgtggggt gagcgactgg ccatatactt ggacggcgcc ctggtggccg
     1081 agaccgccca tcgcggccta ctcgatgcgc cgctcaggga tgctcgcatc gtcctgggcg
     1141 agggcgagtc cgagttcgac ctggatgagg tccgcatctc ggatgtctct cgcgacgcgt
     1201 ttgacctgac gcacgcgccc gaagccgacg cgcacacgct gctcctcgac cacctcgcgc
     1261 tcgatccggg tgcaacccgc aggacgtcgc cggccagagg cgaggggggc gtggcgaccg
     1321 gttgggtcgt cggcgagggc cgtttcgggc cggccctggc ccggccggac gaggccgccc
     1381 agatgaccct tctggaccgc ctcgcccagc tgggcgttcg cacactggtg ttccacgagc
     1441 actggacgga catccagaac tacaccagca ccacccatgg tgaccaactc cgcagcctgg
     1501 tgcgagcgtg ccacgaccgc ggcatgagac tcctgctcta cttcggctat gagatctccg
     1561 acatcgcgcc cgagttcgcc gcctacgccg atgagatgct ggttgcgccc cgccagggcg
     1621 gctacacgcg gcagccgccg cagaccgcct acattgtctg ctaccagagc aagtggcagg
     1681 acttcatggc ccaaggcatc gcccgcgtga tggacgagta cgacatcgac ggcgtctacc
     1741 tcgatggaac cgagtatccc tggggctgcg ccaatacgcg ccacggatgt ggctacacgc
     1801 gccccgacgg caccatcggc accacctgga gcatctacgg ggcgcgggag atgctgcgcc
     1861 ggatctacac catcgtgaga tcgcgcaagc ccgacggcca ggtcaacgtc cacaactcca
     1921 cctgcatggc catgccctcc ctcgcattcg ccacgagtag ttgggacggc gagcagctcg
     1981 gcggcatgga gccgggaccc gacccgctca ccgtcctccc gctcgacacc ttccgctgcg
     2041 agttcatggg gcgccagtgg ggcgtccccg ccgagttcct ctgctacgac cgcccctaca
     2101 cgtggagcca ggccctctct ttcactctgc tgcacgatgt cctggtccga gggagcctgg
     2161 gcggcagcct cgaggccgag tccaagctgt ggcacgccat ggacgacttc ggccggcacg
     2221 aggccacctg gttgccctac tgggataatg gccgctatgt caccctcggg cccgatgcct
     2281 ccatcaagag cagcctctac tggcgcccgg gcaagggcgt ggtcgccgtg gtctccaacc
     2341 tcgggcccaa ggcctcgaat gccgaggtca gactcaacac cgctgccctg aagctcgtcg
     2401 gtgacctcca ggccacagac atcctctctg acgacccggt cgacatcagt cgcgacggcc
     2461 gaatgtcttt cccgctgcct tcctttggct tccgagtcgt gtgggtgcgg gagcagtagg
     2521 gcaagccccg ccggcaacgc cgcgaagccg aggcgggtga ccgtcgccaa tggtacgcgc
     2581 ctgacgccga ttccgacccg cgggccttgc tcagaccaca agctagggtg tcatcctgag
     2641 cgcgtctacg ggcggtgccc gcaggccgag gtccgcagac ggccgcgaac cggggaccgc
     2701 cacgaacgat acgcgcttaa ggccggcacc cgcgcgcccc cgaccgtagg cgaggcttca
     2761 gcctcgcgcg aagtggcttc gcaggccatt gtgagccgag gctaaagcct cggctacgaa
     2821 gggaatcaga catgcgcctt cggagctcgc cagatgcggc cgtgtccttg ggagcgcacc
     2881 gtctgcggag cgaaggatct cggctcgaac cggcggagcg ccgaaggcat cggcccgtgc
     2941 ccacgtctgg aggccgttgc cggccgagat ccttcgtcgc tgacaaacct cgggatgacc
     3001 ccatcgccag tggtgcgagg gagatcccct ggccggctcg cgtgagcgcc gcacccccgc
     3061 ggcgagcccc gggtctgcag cattcgctgc agtcacccat gccttggctt ccgggaccgt
     3121 gactgacgcc ggctcccgcc cggagatccg acccatcccc gcttcccccg cgtcaccggt
     3181 atactcctga caggcgcagc cggcgtgccc ggcgcacttc gaggagccca gggcccatgg
     3241 ccagtctcga catcctccgc gacccggcag agctggagcg tttcgccggc cagtggcccg
     3301 atgcggccca ggctgacgga ggagccgctc tcggcaccag cctgctctgg atcgagtcct
     3361 ggttcgcggc attcggcgcc gagggcgccc ccgagatctg gtcgttctcg gagactgggc
     3421 gctggctggg tgccatcccg ctcgtccgcc gccgcgagcc gagcccatgg cgcgtgtcgc
     3481 cccggctcga tgtcctggcc ggcgccgcca acgtgcatac gcccgactac ggcatcgccg
     3541 gcccatcgcc ggatcgcgtg ctcgatgcct tctgccggga gctggccgca aggcggcacc
     3601 gctgggatcg agtcatcctg acgagaatcc cggcggcatc cgcgctcgcg accgatgggc
     3661 ctcgtgcctt cgcctgccac gggctgagct ggctgcgact ccccacggcc ttccaggcca
     3721 agatccccac cggtcagggc gaaaatgcgt gggcggccgc ccgccgcagc ctcgtcagga
     3781 agaccggcca gtaccgacga gccctggagc gccagggcga cctgcggttc gacatgatct
     3841 gctccccgcc cgggtccgac acggccttcg agcagttcac gaccctcgaa tccgccggct
     3901 ggaagggcgc ccgcgggtcc gccatctcgt cgtcggaggt cacgcgacgc ttctactcga
     3961 ccctggtgcg tcgagctgcc gaggaccgct cgctcc
//
LOCUS       DAOSPL010000228         3993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22717_length_3993_cov_3.383443, whole genome shotgun sequence.
ACCESSION   DAOSPL010000228 DAOSPL010000000
VERSION     DAOSPL010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22717_length_3993_cov_3.383443"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1912
                     /locus_tag="PLQ54_05575"
     CDS             <1..1912
                     /locus_tag="PLQ54_05575"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOS92762.1"
                     /translation="VTLAAQITTLPLIALYFGRLSLVSPLVNFLILPVQSPLLILGGL
                     ATLIGLVPLHLGYGSEILARTKPTLYDLIVALAAGLAAAYATIDPKVSPALVGVAIAT
                     ALVPPLATCGICLAGHRWEWAGGAFLLFAANFLAIETVGAAAFIFVGLHRGTDADNAS
                     AATGVAPASPVRRMLVHIVALMLVGAYMTQTLLGLFRERRLTEGVRTVLRQQVLETTG
                     AWLDDVSHEERQGQLVVTATVISPRPYEAASVARMESALRRKVDARARLIVRSVVSSD
                     SDAHGPVFLRPEDERKREAVDEEARRLSVINDRLSRWLESVRGAVLVDVTQDTGGPTP
                     VVTAVVRTPTAFGPEAVARAQKDLSGVLRAPVRLVVQSVLVRTADADGFLDQRSPPAA
                     ANQAESLQRQRWERVVGAALDSSAPGVLLAGLSATDDGSSISVLAAVLSPSGVTARQV
                     GAAQTHLRLHAHPSASLRVRVVAAGTATATGYEIDPSLDQPLLPAGVAPVALRSEGAP
                     TDPVLDLSNAAADGDEEAVDRLLSKGVDIGSSALFGWTPLHRAAYGGHTGVVKLLLRR
                     GANPTARDTRGASPLHWAVAEGQGDVVRALFYAGADCGAHDSAGGTPSAWAATRGRED
                     MVALLDRLAALR"
     gene            2342..2659
                     /gene="rplU"
                     /locus_tag="PLQ54_05580"
     CDS             2342..2659
                     /gene="rplU"
                     /locus_tag="PLQ54_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOS92763.1"
                     /translation="MYAVVSTGGKQYRVAEGDVLEVERLEGRKGTELTLDRVLMVQEL
                     AETVIGNPTVPDARVVVRIMDQCRGRKIRGFKYKCKSGYKRTFGHRQELTRVRVVRIG
                     RGA"
     gene            2683..2946
                     /gene="rpmA"
                     /locus_tag="PLQ54_05585"
     CDS             2683..2946
                     /gene="rpmA"
                     /locus_tag="PLQ54_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017302787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOS92764.1"
                     /translation="MAHKKGMGSSRNGRDSASKRLGVKMFGGQAARAGSILVRQRGTK
                     FHPGRNVGRGNDDTLFALVDGTVKFEQIGGVRVVSVYPKAAAS"
     gene            3046..>3993
                     /gene="obgE"
                     /locus_tag="PLQ54_05590"
     CDS             3046..>3993
                     /gene="obgE"
                     /locus_tag="PLQ54_05590"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOS92765.1"
                     /translation="MSLFIDEAEITVQGGDGGSGLVSFRREKFMPKGGPDGGDGGDGG
                     DVVLVADKHLGSLLDLTHRHHLKADRGLPGQRNCRSGRSGRALVVPVPVGTTVTDADT
                     GERLGDLTRPGQKLTVAFGGVGGRGNDAFKGPTRQAPMFCEKGERGERRRLRLELKLL
                     ADVGLAGLPNAGKSSLLARVSAARPKIANYPFTTLVPNLGVCRLGRHGSYVIADVPGL
                     IAGAHQGIGLGHCFLRHLERTRVIVHLVDASRTERPDPLEDFATLVSELALFGRGLER
                     LPRLVALNKIDVSDRDDVAALTAELEARGETVFAISAATG"
BASE COUNT          614 a         1288 c         1436 g          655 t
ORIGIN      
        1 tgtcactctg gcggcccaga tcacgacgct gccgctcatt gccctgtact tcgggcgcct
       61 gtcgctggtc tcgccgctgg tcaacttcct gatcctgccc gtccaatccc cgctgcttat
      121 cctgggcggc ctggcgaccc tgatcgggct ggtcccgctg cacctcggct acggatccga
      181 gatcctggca cgcaccaagc cgaccctcta cgacctcatc gtcgctctcg ccgcgggact
      241 cgcggccgcg tacgcgacga tcgacccgaa agtgagccct gcgctcgtgg gggtagccat
      301 tgcgacggcg ttggtgcccc cgctcgcgac gtgcggcatc tgcctcgccg ggcatcggtg
      361 ggaatgggct ggcggcgcgt tcctcctctt cgcggccaac ttcctggcca tcgagacggt
      421 cggcgccgcc gcattcatct tcgtcggcct gcaccggggc accgatgccg acaacgcatc
      481 ggcggcaact ggcgtcgcgc ccgcctcacc ggttcgcagg atgcttgtcc acatcgtcgc
      541 cctcatgctc gttggggcct acatgaccca gacgttactc gggctgttcc gcgagcggcg
      601 actcactgaa ggcgtccgga ccgtactacg tcagcaggtc ctggagacta ccggagcgtg
      661 gctggatgac gtgagtcacg aggaacgcca gggacagctc gtggtcaccg ccaccgtcat
      721 ctcgcccagg ccgtacgagg cggccagcgt cgcccggatg gagtccgcgc ttcggcggaa
      781 ggtggacgcg cgagcgcggc tcatcgtccg gtccgtggtc tccagcgact cggacgcgca
      841 cggacccgtg ttcctccggc cagaagacga gaggaaacgt gaggccgttg acgaggaggc
      901 caggaggctc tcggtcataa acgacaggct gagccgatgg ctcgaaagtg tgcgtggagc
      961 ggtcctcgtc gatgtcaccc aggacacggg gggacccacc ccggtggtca cggccgtcgt
     1021 ccgaactccg acggcattcg gtccggaggc ggtggcacga gcccaaaagg acctgtctgg
     1081 cgttctgcgc gcgccggttc ggctggtggt ccagtccgtg ctggtacgaa ccgccgacgc
     1141 cgacggattt ctcgaccaga ggagcccacc cgccgctgcc aaccaggcgg agtcgctcca
     1201 gcgtcagcgc tgggagcgtg tggtcggcgc cgcgctggac tcgagcgcgc ccggcgttct
     1261 cctcgccggt ctatccgcga cagacgatgg ctccagcatc tcggtgcttg cggcagtgct
     1321 gtccccgagt ggcgttacag cgcgacaggt tggcgccgcg caaacgcatc tgcggctgca
     1381 tgcgcacccc tcggccagcc tgcgagtgcg cgtcgtcgcg gcgggtaccg caacggccac
     1441 ggggtacgag atcgacccgt ctctcgacca gcctctgctg ccggcgggtg tggcgcccgt
     1501 ggccctccgg tccgaagggg cacccactga tccggtcctc gacctgagca acgccgcagc
     1561 cgatggcgat gaagaagcgg tggaccggct gctctcgaag ggggtcgaca ttggctcgtc
     1621 ggctctgttc gggtggacgc ctctccaccg agcggcctac ggggggcaca cgggcgtggt
     1681 gaagctactc ctcaggcgcg gcgcgaaccc aacagcccgc gatacacgcg gagcctcccc
     1741 cctgcactgg gccgtcgcgg agggccaagg cgatgtggtg cgtgctctct tctacgccgg
     1801 cgcagactgc ggggcccatg actcggcggg cggcacgccc tcggcctggg cggcgacccg
     1861 cggccgagag gatatggtag ccttgctcga ccggctggcc gcactgcggt agacgcgtcg
     1921 gcccggacat ggcgtacaga gttcacgcgg cgcgcagggg aggcagcgaa ccacgaggcg
     1981 tgggttccta cgattgtcgt cgacgctgcg ggcagctgcg tggcgtgggt atgcgagagg
     2041 ccgccgcact gaggcggtcc gtgtcatgaa ctgcgtcggg ctgccctgcc ggacccctgc
     2101 ttccgcgcca ccgacggcct cgaatcctgt tgagttcaga caagataagc cacatgccag
     2161 tgcgcgcgtc ccgcgccgcg aatcgcctct ggacacacgc ccgccaacgt ggtaagatct
     2221 gttttcgtcc tgtgtgaccc ggctcgaacc gggtcgctgt gtgttgactg ggcaagcccg
     2281 cgttcgggcc gtggggacgc cggcccgcac tgtgacgcgt cttgagggaa cagcgatggc
     2341 gttgtatgcg gttgtgtcaa cgggtggcaa acagtaccgc gtggccgaag gcgacgtgct
     2401 tgaggtcgag cggctcgagg gccggaaggg caccgagctc acgctggacc gagttctgat
     2461 ggttcaggaa ctggccgaga cggtgatcgg gaatcccacg gtgcccgatg cccgagtggt
     2521 cgtgcgcatt atggaccagt gccggggccg caagatacgc gggttcaagt acaagtgcaa
     2581 gagcggctac aaacgcacat tcgggcaccg gcaggagctg acgcgggtgc gcgtggtgcg
     2641 aatcggccgc ggggcctagc cgaacgaccg tgaggtgaga gtatggcgca caagaaaggt
     2701 atgggaagct cgcgcaacgg gcgcgacagc gcatcgaagc gtctgggtgt gaagatgttc
     2761 ggaggccaag cggctcgcgc gggcagtatc ctggtgcgtc agcggggcac caagttccac
     2821 cccggccgga acgtggggcg cggcaacgac gacacgctgt ttgctctggt cgacggtacc
     2881 gtcaagttcg agcagattgg cggcgtgcgt gtcgtgagcg tgtaccccaa ggccgcggcg
     2941 agctagccgg ctactgccag gtattcagag tacggcattt gtctagatgt ctatacaagc
     3001 ccgggcgcgt cgcgtccggg cttggctgct tggcagggga accccatgtc gctgttcatc
     3061 gatgaagccg agatcacggt ccagggcggc gacgggggca gcggcttggt gtccttccgt
     3121 cgcgagaagt tcatgcccaa gggtgggcct gatgggggag acggcggcga cgggggcgac
     3181 gtggttctgg tggccgacaa gcacctgggc agcctgctgg acctgacgca ccggcaccac
     3241 ctgaaggcgg atagggggct gccggggcag cggaactgcc ggtcggggcg gagcggtcgc
     3301 gcgttggtcg tgccggtccc ggtcggtacg acggtcacgg atgcggacac gggcgagcgt
     3361 ctgggcgatc tgacccggcc gggccagaag ctcacggtgg ccttcggcgg ggtcggtggg
     3421 cgaggcaacg atgcgttcaa ggggccgacg cgccaggcgc ccatgttctg cgagaaaggg
     3481 gagcggggcg agcgccggcg gctgaggctc gaactgaagc tcctggccga cgtcggtctc
     3541 gcgggcttgc ccaatgcggg gaagtcaagt ctgttggctc gggtctcggc ggcaaggccc
     3601 aagattgcca actacccatt caccacgctc gtgccgaacc tgggcgtgtg ccggctcggg
     3661 cggcacggca gctacgtgat cgccgacgtt ccgggactga tcgcgggggc ccaccagggc
     3721 atcggcctcg gccactgttt tctgcggcac ctggagcgga cgcgcgtcat cgtccacttg
     3781 gtcgatgcgt cgcgtacgga gcggccagac cccctcgagg actttgcgac gctggtgagc
     3841 gagcttgccc tcttcggccg cggcctggag cgtctgccac gcctggtcgc actgaacaag
     3901 attgacgtct ccgaccgcga tgacgtggcc gcgctcacgg ccgagttgga ggcccggggg
     3961 gagacggtct ttgccatctc ggctgcaacg ggc
//
LOCUS       DAOSPL010000229         3989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22748_length_3989_cov_4.007372, whole genome shotgun sequence.
ACCESSION   DAOSPL010000229 DAOSPL010000000
VERSION     DAOSPL010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22748_length_3989_cov_4.007372"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1593)
                     /gene="carB"
                     /locus_tag="PLQ54_05595"
     CDS             complement(<1..1593)
                     /gene="carB"
                     /locus_tag="PLQ54_05595"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOS92766.1"
                     /translation="MPRRNDIDKVLLIGSGPIIIGQACEFDYSGTQACKALREEGYQV
                     VLVNSNPATIMTDPELADFTYIEPITARTVERIIATERPNALLPTLGGQTGLNVAVEL
                     SERGVLARYNVEMIGAKRKAIKKAEDRELFKDAMKAAGLEVPGSGLAYNMRGALQIAE
                     DLGFPLIVRPSSTLGGTGAGIALNEPELREIVAKGLELSPFNEVLIEESVIGWKEYEL
                     EVMRDGADNVVIICSIENFDAMGVHTGDSITVAPAQTLTDKEYQRMRDAALRVIREIG
                     VETGGSNIQFAVHPQTGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     IRNDITRETPASFEPTIDYCVVKIPRWAFEKFPGSDPTLTTQMKSVGETMAIGRTFKE
                     ALQKGLRSLEIGRFGFGADGRGRDLASLPMDEICEGLRVPNPDRLFYLRAALCRGVSV
                     DEIYRLSAVDPWFISNLREIVQYEDELRRDSRQLHLDLARADGRGNGDAHGAARVRRA
                     KELGFSDVQIGHLLGTDEMAVRR"
     gene            complement(1700..2890)
                     /gene="carA"
                     /locus_tag="PLQ54_05600"
     CDS             complement(1700..2890)
                     /gene="carA"
                     /locus_tag="PLQ54_05600"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOS92767.1"
                     /translation="MKAYLALEDGLVFEGRSFGAQGTKTGEVVFNTSMSGYQEILTDP
                     SYKGQIVTMTYPLIGNYGVNTEDVESRRSFVEGFVVKECSQIPSNWRSGQSLPDYLAE
                     QGIIGIEGIDTRMLTKHLRTYGAKKGIITTQIDEVEQLVKFAQLSPGLVGVDLVKEVT
                     CDQAYTWPINGQNGQGQLRMRLPGRSPLAPADGDTLLLQPRAISPTPLRVVAIDCGIK
                     LNIVRRAAAYGCDVTVVPADTDADAILRMEPHGLLLSNGPGDPEAVPYVIETARRLRN
                     RLPIFGICLGHQILGLAFGGRTYKLKFGHRGANHPVKNLQTLTVEITSQNHGFAVDMG
                     SLHDEGMIMTHVNLNDDTNEGMRHEVLPVFSVQYHPEASPGPHDADYLFVNFIEAMLE
                     RGAG"
     gene            complement(2903..>3989)
                     /locus_tag="PLQ54_05605"
     CDS             complement(2903..>3989)
                     /locus_tag="PLQ54_05605"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOS92768.1"
                     /translation="QTHKETVETGCRAAQAGGVGALVCMPNTDPLLARPDDVEFVLER
                     AAQLGRGVHVMVAGSVLPCRGAAELSDLAALARAGCVALSDDGFSLQDRELAREALAG
                     CAEVGLPFFAHCEDEALSASAGVLDTATGTEERLTTSCRAEVVAVERNIELADDTGAR
                     LHILHISVAESVDLLRQAKADGIRVTAEACPHHFTLTGEALLEQGSLAKMSPPLRRPR
                     DVRAVTQGLLDGTIDIIASDHAPHTADEKARGLEAAPAGIVGLETMAALAYTALCERH
                     DLGLDEFLRRLAGAPAALVGATVPSLEAGAPARLAVFRPGEWTIDEDWFLGKGRNTPF
                     IGYPVTLRPWVTVLEHALFSATSVSPH"
BASE COUNT          634 a         1320 c         1326 g          709 t
ORIGIN      
        1 acgccggacc gccatctcgt cagtccccag cagatggccg atctggacat cggagaagcc
       61 gagttctttc gctcgtcgga cccgggcggc gccatgcgcg tcaccattcc cgcggccgtc
      121 ggcccgggcc aggtcgaggt gcagctggcg gctgtcgcgg cgaagctcgt cctcgtactg
      181 cacgatctcc cgcaggttcg agataaacca cggatcgacg gccgacagcc ggtagatctc
      241 gtcgaccgac acccctcggc agagggcggc acggaggtag aagagccggt ccgggttggg
      301 gactcgcagc ccctcacaga tctcgtccat cggcagcgag gcgaggtccc tcccgcggcc
      361 atcggcgccg aagccgaacc ggccgatctc aagggagcgc agacccttct gcagggcttc
      421 cttgaaggtc cggccgatgg ccatggtctc gcccaccgac ttcatctgcg tggtgagtgt
      481 tggatcggaa ccggggaatt tctcgaaagc ccaccgtggg atcttcacaa cacagtagtc
      541 gatggtgggc tcgaaagaag ccggggtctc gcgagtgatg tcattacgga tctcgtcgag
      601 cgtgtagccg acggccagtt tggcggcgat cttggcgatg ggaaatcccg tggccttgga
      661 ggccagggcg gagctccggc tcacccgggg gttcatctcg ataaccacca tgcggcccgt
      721 ctgaggatgc acggcgaact ggatattgct cccgccggtc tcgacgccga tctcgcggat
      781 cacgcgcagc gcggcatcgc gcatccgctg gtactccttg tcagtcagtg tctgggcggg
      841 tgccacagtg atgctgtcgc cagtgtggac acccatggcg tcgaagttct cgatggagca
      901 gatgatgacg acgttgtcgg cgccgtcacg catgacctcg agttcatatt ccttccagcc
      961 gatgaccgat tcctcaatga gcacctcgtt gaagggcgag agttccaggc ccttggccac
     1021 gatctcacgc agctccggct cattcagcgc aatgccggcg cccgtgccgc ccaaggtgga
     1081 cgaggggcgc acaatgagtg ggaagcccag atcctccgca atctgcagcg ctccgcgcat
     1141 gttgtaggca agcccactgc ccggcacctc gaggccggcg gccttcatgg cgtccttgaa
     1201 gagctcacgg tcctcggctt tcttgatggc cttgcgcttg gcgccgatca tctcgacgtt
     1261 gtagcgtgcc agcaccccgc gttcggagag ctccaccgcc acgttcagtc cggtctggcc
     1321 gcccagagtc ggaagcagcg cgttgggccg ttccgtggcg atgatgcgct ccaccgtccg
     1381 ggccgtgatg ggctcgatgt aggtgaagtc ggccagctcg gggtcggtca tgatcgtggc
     1441 cgggttgctg ttgacgagga caacctgata gccctcctcg cgcaacgcct tgcaggcctg
     1501 ggtgccggag tagtcaaact cgcaagcctg cccgatgatg atcggtcccg agccgatgag
     1561 caggaccttg tcgatgtcgt tgcgccgtgg catgggatgt cctccgttgg tgctgctgcg
     1621 cggatgggtt gagcctgcca ccggtcgggc cggatggcgg ccttcgtgct ccgcgggccc
     1681 cctatctggc ccgtgctctt cagcccgcgc ctcgctcgag catggcctcg atgaagttta
     1741 caaacagata gtcggcgtcg tgcggtccgg gagatgcctc ggggtggtac tggaccgaga
     1801 acacggggag cacctcatgg cgcattccct cattggtgtc gtcattgagg ttgacgtggg
     1861 tcatgatcat gccctcgtcg tgcaggctgc ccatatcgac ggcgaacccg tggttctgcg
     1921 aggtgatctc gaccgtcagc gtctgcaggt tcttcaccgg gtgattcgcg ccgcggtggc
     1981 cgaacttgag cttgtaggtt cgcccgccga aggccaggcc caggatctgg tggccgagac
     2041 agatgccgaa gatgggcaac cggttcctca gccgccgtgc ggtctcgatg acgtacggca
     2101 cggcttccgg gtcgccggga ccgttcgaga gcagcagccc atgaggctcc atgcgcaaga
     2161 tggcatcggc gtccgtatcg gctggcacca ccgtcacgtc gcagccgtaa gcggccgccc
     2221 ggcggacgat gttgagcttg atgccgcagt cgatggccac caccctcagt ggcgtgggag
     2281 agatggcccg cggctggagg agcagcgtat cgccgtccgc gggggccagt ggcgaccggc
     2341 ccggcaacct catccggagc tggccctggc cgttttggcc attgatgggc caggtgtagg
     2401 cctggtcgca ggtcacctcc ttcaccaggt cgacgcccac caggcccggt gacagttgcg
     2461 cgaacttcac gagctgttca acctcgtcga tctgcgtcgt gatgatgccc ttcttggccc
     2521 cataggtccg caggtgcttg gtcagcatcc gtgtatcgat gccctcgata ccgatgatgc
     2581 cctgttcagc caagtagtcc ggcagcgact gcccgctgcg ccagttggag gggatctggc
     2641 tgcactcctt gacgacgaag ccctcgacga acgagcggcg ggactccaca tcctccgtgt
     2701 tcacgccgta gttcccgatc agcggatagg tcatggtgac gatctggccc ttgtaggagg
     2761 gatcggtcag gatctcctgg tagccgctca tgctcgtgtt gaagacgacc tcgcccgtct
     2821 tggtgccctg ggccccgaaa ctgcggccct cgaagacgag cccatcctcc agagccaggt
     2881 aagccttcac cgtcgccact ccctagtgcg gcgacacaga agtcgccgag aacaacgcgt
     2941 gttcgagcac cgttacccag ggccgcaggg tcaccgggta cccgatgaag ggcgtgttgc
     3001 ggcccttgcc gaggaaccag tcctcgtcga tggtccactc gccaggccgg aagactgcca
     3061 ggcgcgcggg cgccccggcc tccagcgacg ggacggtggc gcccaccaga gcggccggag
     3121 cgccggccag ccgccgcagg aactcgtcga gcccgagatc gtgccgctcg cagagcgccg
     3181 tgtaggccag cgcggccatg gtttcgaggc ccacgatgcc ggctggcgcc gcctcgaggc
     3241 cccgggcctt ctcgtccgcc gtgtgcggcg cgtggtcaga ggcgatgatg tcgatggtgc
     3301 cgtcgagcaa gccctgggtc acggcccgca cgtcccgcgg gcgcctcaac ggcgggctca
     3361 tcttggccag actcccctgt tcgagcagcg cctcgccggt gagtgtgaag tggtgtgggc
     3421 aggcctcggc tgtgacgcgg atgccgtcgg ccttggcttg gcgcagcaga tcgacagact
     3481 cggccacgct gatgtgcagg atgtgcagcc gtgcgcccgt atcatcggcc agctcgatgt
     3541 tccgctccac ggcaacgacc tcggctcggc agctcgttgt caggcgctcc tcagttcctg
     3601 tggccgtgtc gagcacgccc gcggacgccg agagtgcctc gtcctcacag tgggcgaaaa
     3661 agggcaaccc gacctcggcg cagccagcca gtgcctcgcg cgcgagttca cgatcctgca
     3721 gcgagaagcc gtcatcgctc agtgccacgc atccggcgcg agccagggcc gccagatccg
     3781 acagctcggc ggccccgcgg catggcagca ccgagccggc caccatgacg tggacgccgc
     3841 gtccgagctg ggcggcgcgc tcgaggacga actcgacatc gtccggcctc gccagaagcg
     3901 ggtcggtgtt cggcatgcac accagggcgc ccacaccgcc ggcctgagca gcccggcagc
     3961 ccgtctcgac cgtctccttg tgcgtctgg
//
LOCUS       DAOSPL010000230         3987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22765_length_3987_cov_3.108596, whole genome shotgun sequence.
ACCESSION   DAOSPL010000230 DAOSPL010000000
VERSION     DAOSPL010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22765_length_3987_cov_3.108596"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..542)
                     /locus_tag="PLQ54_05610"
     CDS             complement(21..542)
                     /locus_tag="PLQ54_05610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOS92769.1"
                     /translation="MDEEWSARAGRTVVIGVGNRLMGDEGIGVRVVEELKRRSLPDDV
                     DVVDAGTIGLDLLFVLEGYARAVIVDAAEMGLPAGEVRVLGPADLAEAGPVSDAHGMG
                     VAQVLELAEAMGLCPANVVLVGVQPDRVGPALDLSKPVEAALAGAVRCVEALVRTPGD
                     GAARVPRSGPWHE"
     gene            complement(535..1869)
                     /locus_tag="PLQ54_05615"
     CDS             complement(535..1869)
                     /locus_tag="PLQ54_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOS92770.1"
                     /translation="MASVSVNVHNITRVEGHGNIVLNVRDGTIEELRLEITESPRFYE
                     AMLVGRNWDEAQEISCRICGICSTGHSTASLRAMEAALGVTPSEQTVLLRKLALHGEH
                     NQSHVLHVLFLVAPDLLGAPSVVPLASTHRPVIEMALHLKRLANDHIALVTGRHIHGV
                     NLKVNGFHSLPKEKALAALRERLVASRDDIQATIELVASLKLPDFERAGEYVANSRAD
                     EYAFYDGTITSFRPLQCDHCGHTLKEGYREEYPIERYRDIARETVVKHSHAKHTASVH
                     GSYAVGALARVNINYDLLHPAAKQAAEALGFRAPCMNPFMNNVAQLIEVVHCTEDSIA
                     IIDKLLERGIQQEDTSVEVRAGRGAAAVEVPRGILFHEYEVDDDGKIVAANLIIPTGQ
                     NLNNIEHDMRALVPQILTLSHDEIRLMLEMLVRAYDPCISCATHLLDVRFVG"
     gene            2221..3720
                     /locus_tag="PLQ54_05620"
     CDS             2221..3720
                     /locus_tag="PLQ54_05620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOS92771.1"
                     /translation="MLALAQCALVALLAVWLLVLRWPLGYPPEWVWQASAMPTPMHGL
                     FGIARALLASGVLVGAALWMRQRGTHPRFWTAATLLLLVGSVFLCQVALSVGYPGGMA
                     WAVASQFSPVASEYFDSAFRLEDPVSYCRDYLETMRHAGYHLATHPPGMVLLHWVFVV
                     PARDIEIGRALATRFAEGVGGMSLAELGRFARRYPGAAQLPDGAVPAAAAAAVAPSLF
                     GALSLIPLFALVRRLTDISRALWVALLFATAPSLLLLGQCADQWVLFISMVLAWLAVE
                     GITRDHWVWFAAAGVVAGLGTAVTFGFLTCCVAVGVLAVVLSPSRPTVRRLAIRAVAF
                     ASGFAGAWALVSVTLGVNLIAVCEQGLNAHARATLGPQRTHAVWAWLNFVELALFLGL
                     PVAARVASMVWAQLKLWSPRRPMTAGLALAVAGLSAMVLPSALGLVRGEVGRIWLFLM
                     PLLVAGALPAPGPGQPANEVPPWLAMTASLQIAQVLLMAAVMLPMVRPY"
     gene            3776..>3987
                     /locus_tag="PLQ54_05625"
     CDS             3776..>3987
                     /locus_tag="PLQ54_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92772.1"
                     /translation="MQQFVGKTVSVVMEELWGESASIEGELVAVDERGWVIRPGSVTV
                     HDRLRRWYQSTQSDLVYVPQRLIVMAS"
BASE COUNT          584 a         1391 c         1279 g          733 t
ORIGIN      
        1 gcgactctgt tccgagcaac ctactcatgc catggccccg agcggggcac acgggctgcc
       61 ccgtcccctg gcgttcgcac cagcgcctcc acacacctca cggcaccggc cagagccgcc
      121 tccacgggct tcgacaggtc caatgcggga ccaaccctgt ccggctgtac acccacgaga
      181 accacattcg cggggcaaag ccccatggcc tccgcgagct cgagcacctg ggcaacaccc
      241 attccgtgag cgtcggacac gggaccggct tctgccagat cggccggacc cagcacgcgc
      301 acttctcccg ccggcagacc catctcggcc gcatccacga tcacggcccg cgcatacccc
      361 tcgagcacaa acagcagatc cagcccgatg gtacccgcgt ccaccacgtc cacgtcgtcg
      421 ggcagcgagc gccgcttcag ttcctccacc acccggacgc cgatcccctc atcgcccatc
      481 aaccggttgc cgaccccaat gaccaccgtc cgcccggccc gggccgacca ttcctcatcc
      541 aacgaagcgc acatccagca ggtgggtagc acacgagatg cacgggtcgt aagcacggac
      601 cagcatctcc agcatgagcc ggatctcgtc gtggctcagc gtcaggatct gcggcaccag
      661 ggcccgcatg tcgtgctcga tgttgttcag gttctggccc gtcggaatga tgagattcgc
      721 cgcgacgatc ttgccgtcgt catcgacctc gtactcgtgg aacaggatgc ctcggggtac
      781 ctcgacggcg gccgccccgc gcccggcccg cacctcgaca ctcgtgtcct cctgctgaat
      841 cccgcgctcc aggagcttgt cgatgatcgc gatggagtcc tcggtgcagt ggacgacctc
      901 gatgagttgc gccacgttgt tcatgaacgg gttcatacac ggagcccgaa acccgagcgc
      961 ctcggccgct tgcttggctg ccggatgtag caggtcgtag ttgatgttca cccgcgccag
     1021 tgcgcccaca gcataggacc cgtggaccga ggccgtgtgt ttggcatgcg agtgcttcac
     1081 caccgtctcg cgcgcgatgt cgcgataccg ctcaatcgga tactcctccc gatagccctc
     1141 tttgagcgtg tgcccgcagt ggtcgcactg gagcggccgg aacgacgtga tggtgccatc
     1201 gtagaaggca tactcatccg cacggctgtt ggccacgtac tcgcccgctc gctcgaagtc
     1261 aggcagcttc agcgacgcca ccagttcaat cgtggcctgg atatcatctc gcgacgccac
     1321 caagcgctca cgcagggccg caagcgcctt ctccttcggc agactgtgga acccattgac
     1381 cttgagattc acgccatgga tatgccggcc agtcacgagc gcaatgtggt cgttggccaa
     1441 ccgcttgaga tgcaacgcca tctcgatcac cgggcggtgg gtcgacgcca aaggcaccac
     1501 gctcggggcg cccagcaggt cgggggcgac caggaacagc acgtggagca catggctctg
     1561 gttgtgctca ccgtggagag ccagtttccg caggaggacc gtttgctcgg acggcgtcac
     1621 ccccagcgcg gcctccatgg cccgcagcga cgccgtcgag tgcccggtgg agcagatacc
     1681 acagatgcgg cacgagatct cctgcgcctc atcccagttc cggcccacga gcatggcctc
     1741 gtagaagcgc ggggattccg tgatctcaag ccgcagctct tcgatggtgc cgtcgcgaac
     1801 gttcaggacg atgttgccgt ggccttcgac gcgggtgatg ttatggacgt tgacgctcac
     1861 actcgccatg ggggcctccg gagctctcga agcaaaagac ccctcgggac gcacgtgccc
     1921 caggcggcta ctctgctccg gcggtcgcgc tgccgtccag gcctgccacc ggcgggatgt
     1981 gtcacgaggg accgactcgg accactacgc aacaaccatt cccactactg gaccgcattc
     2041 tagcgagcag cccctggcgg aggcaaggca tagttccagc aggccatgtt cggccgccac
     2101 gccgacggca ggctctgcca tctgccatct ggtccggggc acaagggtgc ctgcccccgg
     2161 ccgccgaaca cgccgagcat ggctgaaccg actccgcctt cttcgccgcg cggtgcccgg
     2221 atgctcgccc tggcacagtg cgccctcgtg gccctgctgg ccgtgtggct gttggtgctc
     2281 cgctggcccc ttgggtaccc gccggagtgg gtgtggcagg cgagtgccat gccgactccg
     2341 atgcacggcc tgttcgggat tgcgcgggct ctcctcgcgt ccggtgtcct ggtcggtgcc
     2401 gcactctgga tgcgacagcg tggaacccac ccacgcttct ggactgcggc caccctgctg
     2461 ctactggttg gctccgtgtt cctgtgtcag gtcgccctgt cggtcggcta tccgggtggg
     2521 atggcgtggg ccgtggcctc gcagttcagc cccgtcgcca gcgagtactt cgactccgca
     2581 ttccgcctcg aggatcccgt gagctactgc cgagactacc tcgagacgat gcgccacgcc
     2641 ggctaccact tggcgacaca cccgccgggt atggtgctgc tccactgggt cttcgtggtt
     2701 cctgcccgag acatcgagat cgggcgcgcc cttgccacgc gtttcgccga gggcgtgggt
     2761 ggcatgagtc tggccgagct gggtcgcttc gcccgccgct accctggcgc cgcccagctc
     2821 cccgatggcg ccgtgcccgc cgcagctgcc gcggccgtgg cgccgagtct cttcggggcg
     2881 ctctccctca tcccgctgtt cgcgctggtc cggcggctga ccgacatctc tcgggccctg
     2941 tgggtcgctc tgttgttcgc caccgcgccg agcctgctgc tgttggggca gtgcgccgat
     3001 cagtgggtcc tgttcatctc catggtcctg gcgtggctcg ctgtcgaggg gatcacgcgt
     3061 gatcactggg tgtggttcgc ggctgcgggc gtggtcgccg gactggggac ggcggtcacc
     3121 ttcggctttc tcacgtgttg cgtcgccgtc ggtgttctcg cggttgtcct ctcgccgagc
     3181 cggccgacgg tccgccggct ggcgatccga gccgtcgcct tcgcctccgg gttcgccggt
     3241 gcgtgggccc tggtgtccgt cacactcggt gtcaacctca tcgcggtgtg cgagcagggc
     3301 ctgaatgccc acgccagagc gacactgggc ccccaacgca cccatgcggt atgggcgtgg
     3361 ctcaacttcg ttgaactcgc cctgttcctc gggctgccgg tcgccgcacg ggtggcgtcc
     3421 atggtctggg cgcagctcaa gctctggagt ccgcgacggc ccatgacggc gggcttggcc
     3481 ctggccgtcg ctgggctgtc ggccatggtg ctgcccagtg ccctcggcct ggttcggggg
     3541 gaggtgggcc gaatctggct ctttctgatg ccattgcttg tggcgggtgc attgcctgct
     3601 ccaggtcccg gccagcccgc gaacgaggtg ccgccctggc tggccatgac tgcaagcctg
     3661 cagatcgccc aggttctgct gatggcggct gtcatgctac ccatggtgcg gccgtattga
     3721 ctcggcacgg tccggcagag acactcagtg gcgaagacgg caggaggtcg cggcaatgca
     3781 gcagtttgtg gggaagacgg tcagtgtcgt tatggaggag ctgtgggggg agagcgcctc
     3841 gatcgagggc gagctcgtcg ctgtcgatga gcggggctgg gtgatccggc cgggctcggt
     3901 caccgtccac gatcggctac gccggtggta ccaatcgacc cagtccgatc tggtctatgt
     3961 tccccagcgc ctgatcgtca tggcgtc
//
LOCUS       DAOSPL010000231         3981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22809_length_3981_cov_3.350993, whole genome shotgun sequence.
ACCESSION   DAOSPL010000231 DAOSPL010000000
VERSION     DAOSPL010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22809_length_3981_cov_3.350993"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..1934
                     /gene="ilvD"
                     /locus_tag="PLQ54_05630"
     CDS             261..1934
                     /gene="ilvD"
                     /locus_tag="PLQ54_05630"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOS92773.1"
                     /translation="MGSRPLEGFESTYARALYRAAGHGPRQFGGPLVGIINSYSTANP
                     GHAHLDRLGRVVREAVAGSGGMPIEFGVPAPCDGIAQGPGMHYILPMREAIAAAAELA
                     LQAHSCQAAVMIGSCDKIIPGLLLAAARCNLPTLFVLGGVMPVGQTSLGPMVASDVKE
                     AMGRAAAGTISLGQLDDVEAHACPSPGACNMMGTALTMSCVVETLGLGLPGSATVAAL
                     SDDHLAVAHDAGRRIVALADGGPRVRDCLDMRGIENAIRMGLALGGSTNMVLHVLALS
                     RELGLPLTLGDFDRLSRATPLLTRLKPSSPFTVTDFHGVGGVRALLAELQRAGVLDAT
                     APMAEGGSLGDRLNGVAGADGSVIRPATDPLASEGGLAVLYGNLAPRGAVVKQSGVAP
                     SMRRHRGPARVCESEEHVRERLLSHQVAAGDVLVIRHEGPRGGPGMRELSIPAALLVG
                     MGLGESVAMVTDARYSGATRGPCIGHVCPEAAAGGPLAAVAEGDTIVIDIPGRRLDVE
                     LPDAEIQRRLATYRGPERFASGGFLNIYRALALGADEGAGLEVHHGYEP"
     gene            1924..3369
                     /locus_tag="PLQ54_05635"
     CDS             1924..3369
                     /locus_tag="PLQ54_05635"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HOS92774.1"
                     /translation="MSPDCALYIGGEFCAAASGRTEAVINPATEEPLAHVPYGDATDA
                     RRALDAAAQALGPWGRMNAWERGAILRRAADLIRERAPEIARTMSSEVGKPVPESLGE
                     TLAAAAQFEWMGEEGKRVYGRWVPPSRDGVRRWVLHQPVGVCAAATAWNFPLLLPARK
                     IAAATAAGCTIVARPSIHAPLAAMQMVACLHEAGFPPGVINHVLGPARELMGVFMDDP
                     RCRKVSFTGSTEVGKQLMQGAAGQVKKLSLELGGSAPVLIFPDVDVERVAAATVQAKF
                     RNNGQVCIAAARFYVHQSIYDEYVREVVRRAEALVVGDPMQEGTHCGPLFAREAVERM
                     MSLVDDARTRGARILAGGRPPEGAGFERGFWFLPTVLDQVPPGARLRCEETFGPLMPL
                     FRFGTVDEAIREANDTPYGLAAYVFTRDLSRAIEVCEALRFGIVGLNDMVPATAECPF
                     GGMKESGMGREGGAEGIAEYLETQYVSVGMA"
     gene            complement(3410..>3981)
                     /locus_tag="PLQ54_05640"
     CDS             complement(3410..>3981)
                     /locus_tag="PLQ54_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS92775.1"
                     /translation="IFEPFFTTKPSGEGTGLGLSTVHGVVRSHSGGITVYSEPQVGTT
                     FHVYLPVTTEDTEAPAETGVVLPTGSERVMVVDDEPAVAAMAGEALRALGYEVEEFTD
                     SRKALREFREDPERFDLVLTDQTMPHFTGSQLRASMQFLRPGLPIIIVTGFHPRVRSE
                     PTDGGAATRGALQTLHDLGARPAGSRGAR"
BASE COUNT          582 a         1309 c         1448 g          642 t
ORIGIN      
        1 tagtggctgc agcactcgga tccgatcgct ccgcaggcgc agagcgtcca ccagcgcaat
       61 actgccatgc ccgggtattc caaccacata gggcacgcct gcctgaatca gcagctcggc
      121 gatgatctct ccaccagtca aacgcatcga caacccactc ccacaccaca gaacggaata
      181 gcccaggcgt tcggcgccgg aggttcggga accttgtggg cggggcgatc gcgcgataga
      241 ctgcccggcg gaggatgacc atgggttcgc gtccgctcga gggattcgag tcgacctacg
      301 ctcgcgcact gtaccgcgcc gccgggcatg gcccacggca gttcggcggg ccgctggtgg
      361 gcattatcaa ctcctattcc accgccaacc cgggccatgc gcacctcgac cgattgggcc
      421 gggtggtgcg cgaggccgtg gcggggtcgg ggggcatgcc cattgagttc ggcgttccag
      481 cgccctgtga cgggatcgcc caggggccag gcatgcacta catcctcccc atgcgcgagg
      541 ccattgcggc cgcggcagag ctcgcacttc aagcccacag ctgccaggcg gccgtgatga
      601 tcgggtcgtg tgacaagatc atcccgggtc ttctcctggc agccgcccga tgcaacctac
      661 ccacgctctt cgttctgggg ggcgtcatgc ccgtggggca gacgagcctg gggcccatgg
      721 tggcttcgga tgtcaaggag gccatgggac gcgccgcggc ggggaccatc agcctggggc
      781 agctcgacga cgtcgaggcg cacgcctgtc cgtcgcccgg tgcctgcaac atgatgggca
      841 cggcgctgac catgtcgtgc gtggtcgaga ccctgggcct ggggctgccc ggtagcgcca
      901 cggtggcggc cctctcggat gaccatttgg ccgtggccca cgacgcggga cgccgcatcg
      961 tggctctggc cgatggtgga ccgagggtac gcgactgcct ggacatgcgg ggtatcgaga
     1021 acgccatacg catggggctg gccctgggcg gatcgaccaa catggtgctc cacgtgctgg
     1081 ccctgtcacg cgagctgggg ctgccgctga cgcttggcga cttcgaccgg ctcagccgcg
     1141 cgacacctct cctgacccgt ctgaagccct cgagcccgtt caccgttacg gacttccacg
     1201 gcgtcggcgg cgtgcgtgcg ctcctggcgg aactgcagcg ggcgggggtg ctggacgcga
     1261 cggcgccgat ggccgaaggg ggctcgctgg gggaccggct caacggggtc gccggggccg
     1321 acgggagcgt gatccggccc gcgacggacc ccctggcttc ggagggcggc ctggccgtgc
     1381 tgtacggcaa cctggcgccg cgcggcgcgg tggtgaagca gtcgggagtg gccccctcga
     1441 tgcggcggca ccgagggcct gcccgagtgt gcgagagtga ggagcatgtc cgggagcggc
     1501 tcctgagcca tcaggtcgcg gccggcgatg tgttggtgat ccgccacgag ggcccgcgcg
     1561 gcggcccggg aatgagagag ctctcgatcc ccgcggcgtt gctggtgggc atggggcttg
     1621 gcgaatcggt ggccatggtg accgatgcgc ggtactcggg ggcaacgcgc ggtccctgta
     1681 tcggccacgt gtgccccgag gccgcggccg gaggcccttt ggccgccgtg gccgagggag
     1741 atacgatcgt catcgatatc cccggccggc gcctggacgt ggagttgccg gacgcggaga
     1801 tccagcgacg tctggcaacg taccgtgggc cggagcgctt tgcgtccggt ggttttctga
     1861 acatctaccg cgccctggcc ctgggggccg acgagggcgc cggcctggag gtccaccatg
     1921 gctatgagcc ctgattgcgc cctgtacatc ggtggtgagt tctgcgcggc ggcctccggc
     1981 cgcactgaag cggtgatcaa ccccgcaacc gaggagccgc tcgcacacgt gccctacggc
     2041 gacgcaaccg atgcccggcg agccctggac gcggcggccc aggcgctggg gccgtggggc
     2101 cgtatgaacg catgggagcg gggggccatt ctgcgccgag cggccgatct cattcgcgag
     2161 cgagcacccg agatcgctcg gaccatgtcg tcggaagtgg gcaaacccgt cccggagtcg
     2221 ctgggcgaga ccctggccgc cgccgcccag ttcgagtgga tgggagagga agggaagcgg
     2281 gtctacggca gatgggtccc cccgagcaga gacggggtgc gccgttgggt gctccaccag
     2341 cccgtgggtg tctgtgccgc cgcgacggcc tggaacttcc ccctgctgct gcccgcccgc
     2401 aagatcgcgg cggccacggc cgccggatgc accatcgtgg cgcggccgtc gatccacgcc
     2461 cccctggccg ccatgcagat ggtcgcgtgc ctgcacgagg ccggtttccc gcccggcgtg
     2521 atcaaccatg tgctggggcc cgcccgcgag ctcatgggcg ttttcatgga tgacccccgc
     2581 tgccgcaagg tgagcttcac gggctcgacc gaggttggga agcagctgat gcaaggcgcg
     2641 gcgggtcagg tgaagaagct gtcgctcgaa ctcggcggga gtgccccggt gctgatcttc
     2701 cccgatgtgg atgtcgagcg cgtcgcggcc gcgacggtac aagccaagtt ccgcaataac
     2761 ggccaggtat gtatcgcggc ggcccggttc tacgtgcacc agtcgatcta cgacgagtat
     2821 gtccgcgagg tcgtccggcg cgccgaggct ctggtggtgg gcgacccgat gcaggagggt
     2881 acacactgcg gccccctctt cgcacgggag gccgtggagc ggatgatgtc cctggtcgat
     2941 gatgcccgca cccgaggcgc tcgcatcctg gcggggggcc gccccccgga gggagcaggg
     3001 ttcgagcgcg ggttctggtt cctgcccacc gtactcgacc aggtcccgcc tggggcacgg
     3061 ctccggtgcg aggagacctt cggtccgctc atgccgctgt tccgcttcgg cacggtggac
     3121 gaggccatcc gcgaagccaa cgacacgccg tatggtctgg cggcctatgt gttcactcgg
     3181 gacctgtcgc gggcgatcga ggtgtgcgag gccctccggt tcggcatcgt cgggctcaac
     3241 gacatggtcc ccgccacagc cgagtgcccg tttggtggga tgaaggagtc gggcatgggg
     3301 cgcgaaggcg gcgccgaggg tatcgccgag tatctggaga cccagtatgt ctcggtcggg
     3361 atggcctgac cactactggg actcgacggc gctctggcgc gcattcgcct catcgagcgc
     3421 cgcgcgaacc tgccgggcga gctccgagat cgtgtagggt ttggagagca ccacgggtgg
     3481 cggccccgcc gtctgttggc tcggacctca ctcgagggtg gaaccccgtg acgatgatga
     3541 tcggtaggcc gggtctcagg aactgcatgg acgctcggag ctggctgccc gtgaagtggg
     3601 gcatggtctg gtcggtcagc accaggtcga atcgctccgg gtcctctcgg aattcgcgca
     3661 acgctttgcg gctgtcggtg aactcctcga cctcgtagcc caatgcgcgc agagcttcgc
     3721 cagccatggc ggccaccgcc ggctcgtcgt cgaccaccat gacgcgctcg cttcccgtgg
     3781 gcagcacgac cccggtctcg gcaggcgctt cggtgtcttc cgtcgtcacg gggaggtaga
     3841 cgtggaaggt cgtgccgacc tgcggctcgc tatagacggt gatgccaccc gagtggctgc
     3901 gaaccacgcc gtggaccgtg gacaggccca gccccgtgcc ttcgccgctg ggcttggtgg
     3961 tgaagaacgg ctcgaagatg c
//
LOCUS       DAOSPL010000232         3979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22824_length_3979_cov_3.352192, whole genome shotgun sequence.
ACCESSION   DAOSPL010000232 DAOSPL010000000
VERSION     DAOSPL010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22824_length_3979_cov_3.352192"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2340)
                     /locus_tag="PLQ54_05645"
     CDS             complement(<1..2340)
                     /locus_tag="PLQ54_05645"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOS92776.1"
                     /translation="MVVEPSILVLTALTCASLGAVTDDPTPTAAEIARARQWAAEHLR
                     RPPVPTAAQPPAARRPGLVVLANHGGVLINGRPDGRRLKLGDVEFEHGLICHAPSTVI
                     VRLPSRGRSFSATVGVDANAGGGSIVFSVRVEGREALRTGVLVGGGAGLPVSIDLGGA
                     TEFTIAAGDAGDGIACDHADWAEATVMLADGSELRLSDLPLLEPEPSPRAGSVPPFSF
                     TYGGKHSDDLLPIWTFVESSEELDAQRTRRVQTYTDAATGLELRCVAVEYADFPVVEW
                     TIYFRNTGAADTPIVENLQALDTRFRSTRGGEFLLHHNVGSPCQPNDYEPLETPLGPG
                     ANKRISAAGGRPTNSDLCYFNLDAVNEGLILALGWPGQWSAQFLREDDGALRVVAGQE
                     LTHFVLHPGEEIRTPLVVLQFWCGGDWIRAQNTWRRWMIAHNIPRPGGKLVPMHYGSC
                     FGNLQPLAEEEIGVIRGFIREGIRLDYYFIDAGWYPDRGNWPVTGTWEVDTTRFPNGL
                     REVADLLHDHGMKLVVWFEPERCAGGTWITENHPEWVLGGADGGLVNLGNPEAWQWVV
                     DRVDSLLTSEGIDVYRQDFNIDPLSYWRANDAEDRQGITEIQHVMGYLAFWDELLRRH
                     PNLWIDTCASGGRRNDLETLRRAVPLLRSDYFGTTTAQQAQTMGIAPWMPYFGSGMGM
                     GSVYWYRSCIFPAARVGCDARDPALDYDLLQRMIAECHEVQRHMWGDFYPLTPYSLAE
                     DAWMAWQFHQPDNGEGVIQAFRRAQCPTGSIQVKLRGLDP"
     gene            3016..3591
                     /locus_tag="PLQ54_05650"
                     /pseudo
     CDS             3016..3591
                     /locus_tag="PLQ54_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
     gene            complement(3856..3979)
                     /locus_tag="PLQ54_05655"
                     /pseudo
     CDS             complement(3856..3979)
                     /locus_tag="PLQ54_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018213255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
BASE COUNT          638 a         1286 c         1443 g          612 t
ORIGIN      
        1 tggatccagg ccgcggagct tcacctggat cgagccggtc gggcactgcg cgcggcggaa
       61 ggcctggatg acaccctcgc cgttatcggg ctggtggaac tgccaggcca tccaggcatc
      121 ctcggccagg ctgtagggcg tgagcgggta gaagtcgccc cacatgtggc gctggacctc
      181 gtggcactcg gcgatcatgc gctggagcag gtcgtagtcg agcgcggggt cacgtgcgtc
      241 gcaaccgacg cgggctgcgg ggaagatgca gctccggtac cagtagacac tgcccatacc
      301 catgcccgag ccgaagtagg gcatccacgg ggcgatgccc atggtctggg cttgctgggc
      361 cgtggtcgtg ccgaagtagt cggagcgcag cagcggcacg gcgcggcgca gtgtctcgag
      421 atcgttgcgg cggccgccac tggcgcaggt gtcgatccag aggttcgggt gacggcgcag
      481 gagctcgtcc cagaaggcca gatagcccat gacatgctgg atctcagtga tgccctggcg
      541 atcctcggcg tcattggcgc gccagtagct tagcgggtcg atgttgaagt cctggcggta
      601 gacatcgatg ccctccgagg tcaagaggct gtcgacgcgg tcgaccaccc actgccaggc
      661 ctcgggattg cccaagttca ccaagccgcc atcggcgccg ccgaggaccc actcggggtg
      721 gttctcggtg atccaggtgc cgccggcgca gcgctcgggc tcgaaccaga cgacgagctt
      781 cattccatgg tcatgcagca agtcggcgac ctctcgcagg ccgttgggaa aacgcgtggt
      841 gtcgacttcc caggtgcccg tgacgggcca gttccccctg tcggggtacc atccggcatc
      901 gatgaaatag tagtcgagcc ggatgccctc gcggatgaac ccccggatga caccgatctc
      961 ctcttcggcg agcggctgca ggttgccgaa gcaggaaccg tagtgcatcg ggacgagttt
     1021 cccgcccggg cgcgggatgt tgtgggcgat catccagcga cgccaggtgt tctgggcgcg
     1081 gatccagtcg ccgccgcacc agaactgcag aacgacgagc ggcgtgcgga tctcctcgcc
     1141 cgggtgcagg acgaagtgcg tcagctcctg cccggcgacg acgcggagcg cgccatcgtc
     1201 ctcgcggagg aactgggccg accactggcc cggccagccc agggcgagga tgaggccctc
     1261 attcaccgcg tccaggttga agtagcacag gtcgctgttg gtcggccgcc caccggcggc
     1321 gctgatacgc ttgttggcac cggggccgag cggcgtctcg agcggctcgt agtcgttggg
     1381 ttggcaaggg ctgcccacat tgtgatggag caggaactcc ccgccccgcg tggaccggaa
     1441 gcgcgtgtcg agcgcctgca ggttctcgac gataggcgtg tcggccgctc ccgtgttccg
     1501 gaagtagatg gtccactcga cgacgggaaa gtcggcgtac tcgacggcca cgcagcggag
     1561 ttcgagaccg gtcgcggcat cggtgtaggt ctgaacccgt cgggtccgtt gggcatcgag
     1621 ctcctcggac gactccacga acgtccagat cggcagtaga tcgtcggagt gcttcccacc
     1681 gtaggtgaaa gagaacggcg gtactgagcc cgcgcgtggc gacggctccg gctcgagcaa
     1741 tggcaggtcg gataggcgca gctcggaacc atcggccaac atgaccgtgg cctctgccca
     1801 gtcggcgtgg tcgcaggcga tgccatcgcc ggcgtcgcct gcggctatcg tgaactcggt
     1861 cgcaccaccg aggtcgatcg aaaccggcag gccggcgcct ccgccgacga ggacgccggt
     1921 ccggagcgcc tccctgccct ccacacggac gctgaagacg atgctccccc cgccggcgtt
     1981 ggcgtcgacg ccaactgtgg cggagaagct ccggccgcga gagggcaggc gcacgatgac
     2041 cgtgctgggt gcgtggcaga tgaggccgtg ttcgaactcc acatcgccga gcttgagccg
     2101 gcgtccatcg gggcggccgt tgatgaggac cccgccgtgg ttggcaagga ccacgaggcc
     2161 gggccttcgg gcggcagggg gctgagccgc ggtagggacc ggaggccggc ggaggtgctc
     2221 cgcagcccat tgccgggcgc gagcgatctc agccgcggtg ggagtcggat cgtcagtgac
     2281 ggcacccaag gacgcacacg tcagtgccgt gaggaccaga atgcttggtt ccacgaccat
     2341 gccagcccct tcgtctgcgt tgccgaccgt cggccaccgg tgaaggacgt cacgtggacg
     2401 cgagccgaac cgccaagcgc ggtcgcgcgg catccatgcg gtctcctccg cgcatggaag
     2461 aacaaccccc aaggctcacg gtgtggtacc ctgctgggcg agtctagcgc ggcccctcga
     2521 cggagcgcga gtcagggctc gacgcgcgaa tggccggatt gcctacaata ccagcgcgac
     2581 ggccgcgata ggcatcggcc accagggaca gcgagcggaa caaaccggcg agcacgtccg
     2641 gatgctcccc tgatggcacg tgagtagcat cggcgagacg ctggggacat tcgcgccagc
     2701 agtgctgagg tcctctgacc cgggtaccgg gacctctgcg ggacgggggc ggccatgggt
     2761 atgagagatg acggggaatc ccgcgtacgc cgggcgttcc gcctgatgcg ccgcgctcag
     2821 gggagtaccg tcgagccgtc gcaggcgtcc gaatccgcgg cggatgtgcc cacgacgggc
     2881 accgaggacc tccgggaagc ccaccaagcc ctgctcctgg agatcgagga acggaaacgg
     2941 tgcgaggccg agttggctca ggagcgtgaa ctcacccggg cgctgatcca ctcctctccc
     3001 gccggggtcc tccttatgga ccgccaaggg caagtgcgcg actgcaatga gcgctgtgcc
     3061 gagctacttc atgtgccgcg tgccgccatc gtggggcaga gcgccctgtc actgacaatg
     3121 gacatggcac tgcgctccgc tctcacccga gcgctggtgg gattcggcac cgaacaggat
     3181 ggtaccgtcg ctggacaccc cgacgctccc tcatcgttcg ttcgggcgtc cttcgtggcc
     3241 atccgtggaa acggaggcga gttcctcggg gccgtgggtt tcctgacgga tctctcgcgg
     3301 agccatgatg aggcgtctct gaacgcgcgt ctgcttgcac tggtgaagtc ggccgaggat
     3361 ggggttgtgg gcctcacacc ggagggggtg gtggcgagct ggagtcctcg ggccgaggcg
     3421 atcctggggc cgcggccggg cgaggccatc gggaagccct tcgtcgcggc ggtggcatgg
     3481 gatgacccgg gccaggtgcg cagtctcgtc gacacggccc atggcgggag tgagtcgcgg
     3541 ggagaggtcc gatggcgggc gccgaatgac gagcggcacg gcgcgcgcct gaccgtggcc
     3601 cccgtcgttg gagccggcgg tgtgacgctg gggtcgatcg tgtgggtgcg cgaggcaccc
     3661 ctctcgggcg ccagggtgcc gtcggctgag tgcccaaccg tgcctcgggc catccgatgg
     3721 gagcccggtg tgcccgcagc tccgggcgag cccctcgggg cctcagaggg accggtcgag
     3781 acggccgaca cggcatcgcc gcccgccccg gctgagccgg cttcggaacc ggcgccgagc
     3841 ccggagagcc cgcctgagct cgagcgcgcc gcgggtgagc ctgtgttcac tctcgaaggg
     3901 gttgtaggga tcgctcattg ctccagtgcc aacgacgccg cggcgccgct tcgacctgag
     3961 ctcgcgcgcg atcaacgcg
//
LOCUS       DAOSPL010000233         3961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22949_length_3961_cov_3.241935, whole genome shotgun sequence.
ACCESSION   DAOSPL010000233 DAOSPL010000000
VERSION     DAOSPL010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22949_length_3961_cov_3.241935"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1543)
                     /locus_tag="PLQ54_05660"
     CDS             complement(<1..1543)
                     /locus_tag="PLQ54_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015333797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOS92777.1"
                     /translation="MNAFIRTITSTEPAERDRPFEELCTGLTATELVSACDELDAFRR
                     STENLYHRVRAALFLYAAHRFALMESPGIPQTGVIPQQAFEDLLARRFGQAIDRLLRV
                     QRTEGPNSALSSALAEGYHRLAFQSLVDQVRRSVRASRGNQWMFRTSHVEDHPVRIRQ
                     DLLTRREGSLLYPVLVERTPVRLDLSHSGWSDIFFLGMDYPEGARVLNISVDLGVYGR
                     DRAVRPPIEAYVRVIPEPVLRLTSVDLPATKDITDLRDLFNFGDDYLGLLKAGVIASG
                     VVPPAFEGTGQSLPRLLSRVIGPGMGLELVTNVNDIPKGSRLAVSTNLLACIVSVLMR
                     ATGQTAQLTGPLLERERRLVASRAILGEWLGGSGGGWQDSGGLWPGFKIIQGAVAEPG
                     DPEFGISKGRLLPRHRILGADDLHPATAQQLADSLVLIHGGLAQNVGPILEMVTEKYL
                     LRNQAEWEARQHMREIFDHIVAALRSGDVLELARNTTRNWSGPLKTIIPWVTNAFTET
                     LIARAR"
     gene            complement(1545..2516)
                     /locus_tag="PLQ54_05665"
     CDS             complement(1545..2516)
                     /locus_tag="PLQ54_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOS92778.1"
                     /translation="MQVRKALITAAGRGARLYPAASTVQKAMLPLIDRDGVAKPVIQI
                     IAEEALASGIEEICIICAPGDEAQYLAQFGLLRQNLLAAPHSGPWAEAQADRIGDLLA
                     RLHFAVQEEPLGYGHAVYCGRHFVGAEPFMLLLGDHLYVPRENGKHGAELAIGLATQA
                     KCAVSTVQSTREHLIGNYGTLAGSHVPDLAGVYQIERIIEKPSVSLAELELQTPGLRA
                     GHYLCLLGMHVLTPEILEILEESIERGGAEEGEYQLTPALHELAQRQKCLALEVDGRW
                     CDIGQRYGLLRTQIALGLTGNDQAEVLGAIADLLAEARLGRAMERVS"
     gene            complement(2637..3362)
                     /locus_tag="PLQ54_05670"
     CDS             complement(2637..3362)
                     /locus_tag="PLQ54_05670"
                     /inference="COORDINATES: protein motif:HMM:NF024980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOS92779.1"
                     /translation="MCLVHVCMAKLPRNPQNTAALETCRMEAGPLFEAGMSGSEVARR
                     LGVSAHAACGGRRKWQEGGVEALRSIGPRGYRPALGAEELRILREELLRGPRAHGYST
                     DLWALTRIVQVIKKRFGVQRSVTQVWQIMQQMGFSCQKPSRRAKQRNEEWIAAWQAEE
                     WPRVLGGALNAHATVVFVDESGCSQRPNVRQLRPHLRWTVVVWANLRGQNWPTTRPIV
                     WARSPRRSPAANAARAEPTMAPT"
     gene            3582..>3961
                     /locus_tag="PLQ54_05675"
     CDS             3582..>3961
                     /locus_tag="PLQ54_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92780.1"
                     /translation="MRIAEQHWSGEGIRTAKGLVTGPIVLFRGAAGEVHRDLEAFAGL
                     AGDAAGRQWSRPVTVVEEGDASWDPIVMGLRGALVPHLGDLAELLEVSQVECTEDRAT
                     VSVTLWATGAAPQTGEAWLSAVRM"
BASE COUNT          633 a         1289 c         1353 g          686 t
ORIGIN      
        1 gccgggcgcg cgcgatgagg gtctcggtga aggcgttggt gacccatggg atgatggtct
       61 tcagggggcc ggaccagttg cgggtggtgt tgcgcgccag ctcgagcaca tcgccggatc
      121 gcagcgccgc cacgatgtgg tcgaagatct cgcgcatgtg ctgacgggct tcccactcgg
      181 cctggttccg cagcaggtat ttctcggtga ccatctcgag gatcggcccc acgttctgag
      241 ccaatccgcc gtggatcagg accagggagt cggccagctg ctgggccgtg gccgggtgga
      301 ggtcgtcggc acccagaatc ctgtgccgcg gcagcaggcg gcctttgctg atgccgaact
      361 cggggtcacc gggctcggcg accgcccctt ggatgatctt gaaccccggc cagagcccgc
      421 ccgagtcctg ccagccgccg cccgagcccc ccagccactc ccccaggatg gcccgtgagg
      481 ccaccaggcg ccgctcgcgt tcgagcagag gccccgtaag ctgcgcggtc tgccccgtgg
      541 cgcgcatgag gacactcaca atgcacgcca gcagattcgt ggacaccgcc agccgcgacc
      601 ctttggggat gtcgttcaca ttcgtgacga gctccagtcc catacccggc ccgatgacgc
      661 gcgagagcag gcgcggcagc gactgccccg tcccctcgaa cgccgggggg accacgccgg
      721 aagcgatgac acccgccttc agcagcccca ggtagtcgtc gccgaagttg aacaggtcac
      781 gaaggtccgt gatgtccttc gtggccggca ggtcaacact cgtgagccgc agtacgggct
      841 cggggatgac acggacatag gcctcgatgg gtggtcgaac cgctctgtcc cgtccgtaga
      901 cgccgaggtc aacggagatg ttgaggactc gcgcaccctc ggggtaatcc atgcccagga
      961 agaagatgtc cgaccagcct gagtggctga gatcgagccg gaccggcgtt cgctcgacca
     1021 gcacgggata caggagggat ccctcgcggc gtgtgagcag gtcttgccgg atccgcacgg
     1081 ggtgatcctc gacatggctg gtgcggaaca tccactggtt tccgcggctg gcacggacac
     1141 tgcgccgcac ctggtccacc agactctgga aggcgaggcg gtggtagccc tcggccaagg
     1201 cgctggatag ggccgagttt ggcccctcgg ttcgctgcac gcggaggagc cggtcgatgg
     1261 cctgaccgaa gcggcgggcc agcaggtctt cgaatgcctg ctgggggatg acgccagtct
     1321 gggggatgcc gggcgattcc atgagggcga accggtgcgc ggcgtacagg aagagggccg
     1381 cgcggacccg gtggtagagg ttctccgtcg accggcggaa cgcgtcgagc tcatcgcagg
     1441 cggagaccaa ttcggtggct gtgagccccg tgcagagctc ctcgaagggg cgatcccgtt
     1501 ccgcaggctc cgtactggtg atggtgcgga tgaaagcgtt cacgctagct cacccgctcc
     1561 atggcgcggc ccagcctggc ttccgccagg agatcggcga tggcgcccag gacctctgcc
     1621 tgatcgttgc cggtcaggcc cagggcgatc tgagttcgga ggaggccata gcgctggccg
     1681 atgtcgcacc accggccgtc gacctccagc gccaggcatt tctgccgttg ggcgagttcg
     1741 tggagggcag gagtgagttg gtactccccc tcttcggcgc cgccgcgttc gatgctctct
     1801 tcgaggatct cgaggatctc gggcgtcagc acatgcatcc caagcaggca caggtagtgc
     1861 ccggcgcgca gaccgggggt ctggagctcg agctcagcca ggcttaccga aggcttctcg
     1921 atgattcgct cgatctggta caccccggcc aggtcaggga cgtgggatcc cgctagcgtc
     1981 ccgtagttgc cgatcaggtg ttctcgggtc gactggaccg tggagacggc gcatttcgct
     2041 tgggtggcga gcccgatcgc caactcggcg ccgtgcttcc cgttctcgcg gggcacatag
     2101 aggtgatcgc cgagtaggag catgaagggc tcggcgccca cgaagtgccg gccgcagtag
     2161 accgcgtgcc cgtagcccag gggctcctcc tggaccgcaa aatgcagtcg tgccagcaga
     2221 tccccgatcc gatccgcctg ggcttcggcc cacgggccgc tgtgaggcgc ggccaggagg
     2281 ttttggcgga gaaggccaaa ctgggcgagg tattgagcct catcgccggg ggcacagatg
     2341 atgcagatct cctcgatgcc gctggcgagg gcctcctcgg cgatgatctg gatgaccggc
     2401 ttcgccacgc cgtcgcgatc gatgaggggg agcatggcct tctggacagt gctcgcggcc
     2461 gggtatagcc gggcacctcg cccggcggct gtgatgagcg ctttgcggac ctgcatggct
     2521 tgttcctctc gtggcagcag gtgctcaccg cccctgcggc agagcaggga atgcctggta
     2581 tacgacacca acctgcagat tggtggtcct gagatccagg agtccacagt gcttggtcag
     2641 gttggcgcca tggttggctc ggcgcgtgcg gcgttggccg cgggcgatct gcgcggtgag
     2701 cgcgcccaga ctatcgggcg tgtggttggc cagttctggc cgcgcaggtt cgcccacacc
     2761 actaccgtcc accggaggtg cggtcgcagc tgtcgcacgt tcggacgctg ggagcagcca
     2821 ctctcgtcga cgaaaaccac ggtggcatgc gcgttcagcg ccccccccaa gacgcgaggc
     2881 cactcctcgg cctgccatgc cgcgatccac tcctcattgc gctgtttggc gcgccgcgag
     2941 ggcttctggc aactgaaacc catctgctgc attatctgcc acacctgggt cactgaccgc
     3001 tggacaccaa accgcttctt gatgacctgg acgatccgag tcaaagccca caggtcggtg
     3061 ctgtatccat gggctcgcgg gccccgcagc agttcctccc tcagtatccg tagctcttcg
     3121 gcacccagag cgggccggta gccccgcggt ccaatgctcc gcagcgcctc gacccccccc
     3181 tcctgccatt tcctccgccc gccacacgcg gcgtgggcac tcacgcccag cctccgggcc
     3241 acctcgctgc cggacatgcc ggcctcgaac agcgggcctg cttccatgcg acaggtttcc
     3301 agcgccgccg tgttctgtgg gtttctcggt agcttggcca tgcatacatg tactagacac
     3361 atccactccg tgcctacatt cccaaaacgg gaatgtcatt agtccgcatc ccccgttccg
     3421 tcgtgttgtc ggcccacaca cgacaagcgc atgcgcctcg acctatactc tgtcatggac
     3481 ccaagagctc catcctcgtg caggcgccgc catcaagagg atcagtggtg ttgcggcggt
     3541 cggcctgtag gagccgaggg gtaccaaggg agctgaaggg tatgcggatc gccgagcagc
     3601 actggtcagg ggaaggaatc aggaccgcca agggcctggt aaccggaccc atcgtcttgt
     3661 tccgaggcgc tgctggcgag gtgcaccggg acctcgaggc attcgcgggt cttgccggtg
     3721 atgccgcggg gcggcagtgg agccgtccgg tcactgttgt cgaggagggg gacgcctcgt
     3781 gggatcccat cgtgatgggt ctgcgcggcg cgctggtccc tcacttgggt gacctggcgg
     3841 agctgctgga ggtctcccag gtcgaatgca cggaggatcg ggccacggtc agcgtgacgc
     3901 tgtgggcaac tggcgcggcg ccgcagaccg gcgaggcatg gctgtcggcc gtccggatgg
     3961 a
//
LOCUS       DAOSPL010000234         3959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22961_length_3959_cov_3.524846, whole genome shotgun sequence.
ACCESSION   DAOSPL010000234 DAOSPL010000000
VERSION     DAOSPL010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22961_length_3959_cov_3.524846"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PLQ54_05680"
     CDS             <1..529
                     /locus_tag="PLQ54_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOS92781.1"
                     /translation="VVNERMETGVPGVYAIGDVLDAKWRFAHVAEAEAKVAVRNALGQ
                     DARADYRVVPSTIFTDPLVMSVGARESDLSREDYVVGKAPFRYNSMANCSGDTRGFVK
                     ILVERGAPHRILGAAAVGQDIEIHQMAMAMASGATVHDVSEMIHFHPSRGEAVKQAAE
                     AAVRRLEAEARRAGR"
     gene            735..2045
                     /locus_tag="PLQ54_05685"
     CDS             735..2045
                     /locus_tag="PLQ54_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS92782.1"
                     /translation="MLTRICVRGYKSLEHLDLCLGPLTVIFGPNAAGKSNLLDALQLL
                     SRVGTRPTLGEAFGPPHRGDALHSFHYGKDGIAGLLRHERVGLTIEADLQLSTHTVER
                     VNAEIEGLRQVPRGDNGPGVRRGAQGGVRERRLRYRVSIEVTPADGRLRVADENLAAL
                     SDSGEPSRKRQPFISADGDEIRLRAEGRGHPTYHQRYLDHTVVSLPLYAPHYPHVAAL
                     RRELENWRFFYLEPRERMRAPSSVAEVHHIGPMGENLASFLNTLRALHPGQFQAIERA
                     LSAIVPTVTGLHVEPNRFGQLELSLLEGDAAIPATLASEGTLRVLGLLALCGAQEPPS
                     LIGFEEPENGIHPRRLRHVASLLETRASSETQIIVTTHSPLLPDLVPEPSLYVCSRRS
                     GATTVEPLAYGGLFRASAVTSALDDEAASPTSVCDRILRGDFDD"
     gene            2038..2628
                     /locus_tag="PLQ54_05690"
     CDS             2038..2628
                     /locus_tag="PLQ54_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92783.1"
                     /translation="MTDIALFCEDLGHEVVLGALVRRVARDMGVAAAVAIRSARGGRG
                     RALASLSDYQEDVKRGVARPVDVLVVGLDANCHGYARRRDEVLAHVSSAPLSCVLAVP
                     DPHVERWLLLDGQAFRKVLGHGCQPPDQKCEKARYKALLREAVRAAGVEPVLGGLEHA
                     DQLVAAMDLNRAAHLDRSLGRFLADLQAALRARKQG"
     gene            complement(2662..>3959)
                     /locus_tag="PLQ54_05695"
     CDS             complement(2662..>3959)
                     /locus_tag="PLQ54_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92784.1"
                     /translation="SLIDLADAVRAEAPETFVMWYWGLGSPLWVLYGDTVFESGLFME
                     GSGTSSTPTLYYRDSVTLAQDQNAHHARLIPPRCKDSLGVWLADTRWGNYMGRERWRE
                     ALVMDLGRGSLLFPNIWGDVYLLDDADIGFLAWISRLAKENEQAFLHRRAIGGDPARN
                     EPYGYADFAHDHGYLFVNNAHFAARPIHLELGEPLGLRAAPGAPIRITSLFPDRARVL
                     FPDGAQPRAGQTLDLWLRPFEVLMLEVDAGLPETGDLPARLTTAAQTEGFGEPLVLEP
                     LEEGGAPVEFTDAERFRAQGFEQRTVALRTTLPDLTGPQPILAVPIRLRRDGVEWRHS
                     PCVAEVVQVTAQIGGQQLQLMPVPDARQFGNTQKAGCSWVVYKSRLSREWSGQELRLT
                     VSAWLPPDVAAEPEAWLVKRWWQESTRPVPEGYFTDAPS"
BASE COUNT          610 a         1323 c         1411 g          615 t
ORIGIN      
        1 cgtggtcaac gagcggatgg agaccggcgt gccgggtgtc tatgccatcg gcgatgtgct
       61 cgacgccaag tggcgtttcg cccacgtggc cgaggccgag gcgaaggtcg cggtgcggaa
      121 cgcgctgggc caagacgccc gggccgacta ccgtgtggtg ccgagcacca tcttcaccga
      181 ccctctggtc atgagcgtgg gcgcgcgcga gtcggacctc agccgagagg actacgttgt
      241 cggcaaggcc ccgttccgct acaactccat ggcgaactgc agcggcgaca cacgaggctt
      301 cgtgaagatc ctcgtggagc gcggcgcgcc gcaccggatc ctcggggccg cggcggtggg
      361 tcaggatatc gagatccatc agatggccat ggccatggct tcgggcgcga cggtccacga
      421 cgtgtccgag atgatccact tccacccgag ccgcggcgag gcggtcaagc aagcagccga
      481 agcagccgtc cggcgcctcg aggcagaagc gcggcgggcg ggaaggtagg ggggcgcggg
      541 gcgcctctgg gggcccggct gcccacccgt gcacttcccc ggggctccgc ccccagagcc
      601 ccgcacgagg ggccagcccc tcgtggctcc catgcgttgg cgcaggcggg ggcctcgtcc
      661 ggaaaaggag ttggcctcct tgccgcgcaa taccaggcgg tagccgtggc cacgagggcg
      721 ggaggcggac gaccatgctc acacggatct gtgtgcgggg gtacaagtcg cttgagcacc
      781 tcgatctgtg tctcggaccc ctcacggtca tcttcggtcc caacgcggcc ggcaagagca
      841 acctgctcga tgctctgcag ctgctgtcca gggtaggcac tcggccgact ctgggtgagg
      901 cgttcggtcc gccgcaccgc ggggatgcgt tgcactcatt ccactacggt aaagacggaa
      961 tcgccggcct tcttcggcac gagcgggtcg ggctgacgat cgaggcagac ctgcagctct
     1021 ccacgcacac ggtcgagcgt gtgaatgccg agatcgaggg tctgcggcaa gtgcctcgcg
     1081 gcgacaatgg ccccggtgtg cgtcgcggag cccagggggg agttcgcgag cggcggctgc
     1141 gttaccgggt gtcgatcgag gtcacgccgg ccgacggtcg tctgcgggtc gccgacgaga
     1201 acttggccgc cctgagcgac tcgggcgagc cctcgaggaa gcggcagccg tttatcagtg
     1261 ccgatgggga cgagatccgg ctccgggccg agggcagggg ccacccaacc tatcaccagc
     1321 gctacctgga ccacacggta gtgtcccttc cgctctacgc gccccactac ccccacgttg
     1381 ccgctctacg gcgcgagctg gagaactggc gtttcttcta cctcgagccc cgtgagcgca
     1441 tgcgggcgcc ttcgtcagtg gccgaggtgc accatattgg gcccatgggc gagaacctgg
     1501 cctcgtttct gaacaccttg agagccctgc acccgggtca gttccaggcg attgagaggg
     1561 cgctctcggc catcgtgccg acggttaccg gcctgcacgt cgagcccaat aggttcggcc
     1621 agctcgagct ctcactgctt gagggtgacg cagccatccc ggcaaccctt gcctcggagg
     1681 gcacgctccg cgtgctgggg ttactcgctc tgtgcggagc gcaggaaccg ccgtcgctga
     1741 tcggcttcga ggaacccgag aacggcatcc atcccaggag gctccggcat gtcgccagcc
     1801 ttctggagac acgagcctcg agcgagacgc agatcatcgt aacgacccac tcgccccttc
     1861 tccctgacct ggtccccgag ccctcactct acgtgtgctc caggcgatcg ggtgcgacca
     1921 ccgtcgagcc gttggcctac ggtggactat tcagggcctc ggccgtgacg tcagcgctcg
     1981 acgacgaggc ggcctcgccg acatccgttt gcgaccggat cctccggggc gacttcgatg
     2041 actgatatcg cgttgttctg cgaggacctc ggtcatgagg tggtgcttgg cgctctggtt
     2101 cgccgcgtgg cgcgcgacat gggagtggcg gccgcagtcg ccattcggag cgctcgcggg
     2161 ggaagaggcc gagctctggc atccctgtcg gactaccaag aagacgtcaa gcgcggggtg
     2221 gcacgccccg tcgatgtgct cgtcgtgggg ctggatgcca actgccacgg gtacgcgcgg
     2281 aggcgggacg aggtgctcgc acacgtgtcc agtgcgccgc tgtcgtgcgt tctggccgtg
     2341 ccggaccctc acgtggagcg atggcttctt ctggacgggc aggcgtttcg gaaagttctc
     2401 gggcacgggt gccagccacc tgaccagaag tgtgagaagg cccgctacaa ggccctgctc
     2461 cgagaggccg tgcgggccgc cggcgtcgag cccgtgctgg gcgggctcga gcacgccgat
     2521 cagttggtgg cggcgatgga cctgaaccgg gcagcgcact tggacaggtc actcggccgg
     2581 ttcctggccg acctccaggc cgctctcagg gcccggaagc agggctgatc ccgcgatggg
     2641 cgccccggcc cctccggcgg ctcacgatgg cgcgtcggtg aagtagccct cgggcaccgg
     2701 gcgcgtcgac tcctgccacc agcgctttac gagccaggcc tccggctcgg cggcgacatc
     2761 gggcgggagc caggcgctca cggtgagccg aagctcttgc cccgaccact cgcggctcag
     2821 gcggctcttg tagaccaccc acgagcagcc ggccttctgc gtgttaccga actgccgcgc
     2881 gtcgggcacg ggcatgagct ggagctgttg gccgccgatt tgcgcggtca cctggacgac
     2941 ctcggccaca cagggcgagt gccgccactc gacaccgtcc cgccgcaagc ggatcggcac
     3001 ggccaggatc ggctgggggc cggtgaggtc ggggagcgtc gtgcgcagcg ccaccgtccg
     3061 ctgctcgaac ccctgggcgc ggaagcgctc cgcatcggtg aactcgacgg gtgccccacc
     3121 ctcctcgagg ggctccagca ccaggggctc gccgaagccc tccgtctgcg ccgccgtggt
     3181 gagacgggcc ggcaggtcgc ccgtctccgg caggccggca tccacctcaa gcatgagcac
     3241 ctcgaacggc cgcagccaga ggtcgagcgt ctggcctgca cggggctgag cgccgtcggg
     3301 gaacaggacc cgcgcgcggt cggggaagag gctggtgatg cggatcggcg cgccaggggc
     3361 cgctcgcagg ccaagcggct cgcccagctc caggtggatc ggccgcgcgg cgaagtgggc
     3421 gttgttcacg aacaggtagc cgtggtcatg ggcgaagtcc gcatagccgt agggctcgtt
     3481 ccgggccggg tcgccgccga tcgcgcgccg atgcaggaag gcctgctcgt tttccttcgc
     3541 aagcctgctg atccaggcga ggaagccaat gtcggcatcg tcgagcaggt agacgtcgcc
     3601 ccagatgttg gggaacagca ggctgccgcg gcccaggtcc atgaccagcg cctcgcgcca
     3661 gcgctccctg cccatgtagt tgccccaacg ggtgtcggcg agccagaccc cgaggctgtc
     3721 cttgcagcgc ggcgggatga gccgggcgtg gtgggcgttc tggtcctggg ccagcgtaac
     3781 cgagtcacgg tagtagagcg tgggtgtcga gctggtgccg gagccttcca tgaacaggcc
     3841 ggactcgaac acggtgtcgc cgtagagcac ccagagaggt gagccgaggc cccaatacca
     3901 catcacgaag gtctcgggcg cttccgcgcg gaccgcgtcg gcgaggtcga tgagggagt
//
LOCUS       DAOSPL010000235         3957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_22973_length_3957_cov_4.336750, whole genome shotgun sequence.
ACCESSION   DAOSPL010000235 DAOSPL010000000
VERSION     DAOSPL010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22973_length_3957_cov_4.336750"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2130)
                     /locus_tag="PLQ54_05700"
     CDS             complement(<1..2130)
                     /locus_tag="PLQ54_05700"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOS92785.1"
                     /translation="MRTHSAVHPAHALTLRTGMAVALVFTLQLPLRAQHRAPNVALAS
                     LGASAEASSQFDPVLSPANAINGTWHTWASSWSSGHGMPQWLTVTLKQPQTVQAVRIH
                     ADERPELRFELRDYTVDVRPPGTAEWRTVARVTGNTAAHRLDAFPPVQAEAVRLTVTA
                     TGGGDDVVRMNEIEVFGLGELPETQTAFAVGAIDGQYSEFAIAGDYAQFAERFPDDVT
                     VDLAGADAASTVPYILPGPQDGWAGAKPHCLEVRFPLDHVPAGEGHLLIALVGSQGQY
                     GQRQRPEINGEAATVVFPPQGTSDGVLSDPGQTRPDWRGITFSTELLRPGENTIRLTS
                     VGGSWAVLDGITLTLVEGESGAPRLPTMSVEPTPLLKDVDGRLMQALRVVVTNRGRAV
                     EGTLESAPAGGKRFRPLSHVRVPFGETARFAFIKPVEVETQLNVRFRGAGTELSSPAT
                     IRPPRKMTLYIAPSVHTDIGYTHLQQDVYEVQAKVLDDAVRFCEETATYPDDARFRWN
                     LETAWQVDNYRRKRGEAALARVMELVRQGRMEVSGLYLNMLSALCGHDELCELTARAR
                     EIRERYGAPVACATTTDNPTYTWAYPGLLNNSGIRYLAMGCNETRGPFISNGGGMPPI
                     WWQGYDGQKVLMWFANGYAHASSFICTEEEMEGRVLSYLAPFSEDRYPYDMVLAYGGF
                     GDNSLIARGFCDVVRAWNEKWAYPRLRV"
     gene            2456..2848
                     /locus_tag="PLQ54_05705"
     CDS             2456..2848
                     /locus_tag="PLQ54_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS92786.1"
                     /translation="MVPFALRILVADDEELIREALVDVLGATQHTVVAAADGQVAIEA
                     LASQEFDLVFTDVVMPRATGIQVLEAALSQKRPPRVVVVSGKEIAHSERDFLDYGAYA
                     YFRKPYRLADILSVTQRVAAELAAVCAR"
     gene            complement(3016..3753)
                     /locus_tag="PLQ54_05710"
     CDS             complement(3016..3753)
                     /locus_tag="PLQ54_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOS92787.1"
                     /translation="MDGDNDVAANLQKIAALAHEADPAVPIYSSTWRFSQRLVGALDI
                     WGTGPQGTFPVDEMARRRAAGETLWFTTDGQMCIDTPYCAIERLLPYFCFRYDHAAYE
                     FWGVSWWTYNPWERGWHQYIRQSDEGKEYYYIRYPNGDGYLTYPGDAVGVKGPVSSIR
                     LEQAREGMEDYEALRILQQRVDEARAAGRKTADADAALAAARALVSIPNNGGRFSSEL
                     LPDPEAVSRIRERVLRAADRLERANAE"
BASE COUNT          603 a         1297 c         1395 g          662 t
ORIGIN      
        1 cacgcgcagt cgagggtagg cccacttttc gttccaggcc cgcaccacgt cgcagaaccc
       61 tcgggcgatc agcgagttgt caccgaagcc gccgtaggcc agcaccatgt cataggggta
      121 ccgatcctcc gagaacgggg ccagatagct gaggacccgg ccctccatct cctcctccgt
      181 gcagatgaag ctgctggcgt gggcgtagcc gttggcgaac cacatgagca ccttctggcc
      241 gtcgtagccc tgccaccaga tgggtggcat gccgccgccg ttgctgatga agggcccgcg
      301 ggtctcgttg cagcccatgg ccaggtagcg gatgccgctg ttgttaagca gcccggggta
      361 ggcccaggtg tacgtggggt tgtccgtcgt ggtggcgcag gccaccggcg cgccatagcg
      421 ttcgcggatt tcgcgggccc gggccgtgag ctcacacagt tcatcgtgcc cacacagggc
      481 cgagagcatg ttcaggtaga gcccggagac ctccatgcgc ccctggcgca ccagctccat
      541 cacccgggca agggcggcct ctccgcgctt ccgccggtag ttgtcgacct gccacgcggt
      601 ctccaggttc caccggaacc gggcatcgtc gggataggtc gccgtctcct cgcagaaccg
      661 cacggcatca tcgagcacct tggcctggac ctcgtacacg tcctgctgta ggtgcgtgta
      721 gccgatgtcg gtgtggacgc tcggcgcgat gtagagcgtc atcttccggg gcggccggat
      781 ggttgcgggg ctcgagagct ccgtgccggc gccgcggaag cgcacattga gctgggtctc
      841 gacctccacg ggcttgatga aggcgaaccg tgcagtctcc ccaaagggga cgcgcacatg
      901 agacagcggc cggaaccgct tgccgcctgc cggcgccgac tcgagcgtgc cctccaccgc
      961 gcgcccgcgg ttggtgacca cgacgcggag cgcctgcatc aaccggccgt cgacgtcctt
     1021 gaggagcggt gtgggctcga ccgacatggt cggcaatcgc ggtgcaccgc tctcgccctc
     1081 gaccaacgtc agcgtgatgc cgtccagcac ggcccaggat ccgcccaccg atgtgagccg
     1141 gatggtgttc tctccgggcc tgagcagctc ggtgctgaag gtaatgcctc gccagtcggg
     1201 ccgggtctgg cccgggtcgg acagcacgcc atcggacgtg ccctggggcg ggaacacgac
     1261 cgtcgccgcc tcgccgttga tctcgggccg ctgccgctgg ccgtactggc cctgcgagcc
     1321 gaccagggca atcagcagat ggccctcgcc cgcggggaca tgatccagcg ggaacctgac
     1381 ctccaagcag tggggcttgg cgccggccca gccatcctgt ggcccgggca ggatgtaggg
     1441 cacggtggac gcggcatcag cgcccgcgag gtcgacggtc acgtcgtcgg ggaagcgttc
     1501 cgcgaactgc gcatagtcgc cggcgatggc gaactcgctg tactgcccat cgatggcccc
     1561 gacagcgaac gcggtctgag tctcgggtag ctctccgagc ccgaacacct cgatctcgtt
     1621 catgcggacc acatcatcgc cgccccccgt cgcggtgacg gtgagcctca cggcttccgc
     1681 ctgtacgggc gggaaggcgt cgagccggtg cgccgccgta ttgcccgtga cgcgcgcgac
     1741 cgtgcgccac tcggcggtgc ccggtggacg aacgtcgacg gtgtagtcgc gcaactcgaa
     1801 gcgcagctcg gggcgctcat cggcgtggat gcgcacggcc tggactgtct gcggttgctt
     1861 gagcgtgacc gtcagccact gaggcatgcc gtggccggac gaccacgagc tcgcccatgt
     1921 gtgccaggtt ccgttgatgg cgttggccgg ggagagaacg gggtcgaact ggctggaggc
     1981 ttccgccgag gcgcccaggg aggccaaagc aacgttgggc gcgcgatgtt gggcccgcaa
     2041 tgggagctga agtgtgaaga cgagcgccac ggccataccc gtgcgtaggg taagagcgtg
     2101 agcggggtgg actgcggagt gggttcgcat gcctagcccc ccggagcgat gcgggtcggc
     2161 cgcacggctc gggaactcgc gcggcgagac gcgtcgtgcc ctacgcgtgc ggacacgcag
     2221 gcgatgcgac gcttcgtggg ggcgtgactg atgtcctgcg ggcgggttgc ctccgtgcgg
     2281 cgtaactgaa ggttcgcctt gtggctgtgt aatggtgctg tggtcctttc gtaaaatggt
     2341 acgggtaggt ctgccgaaag acgcgtgctg tggtagtctg tgcgtgacgt cattggctcg
     2401 gggagttcga gctgcgtccg ggggggcgcc aggagggtta gggggcaccg ccagaatggt
     2461 cccatttgcc ttgcgtatcc tcgtcgccga cgacgaggaa ctgattcgcg aggccctggt
     2521 tgacgtactc ggcgccacac agcatacggt tgtcgccgca gcggatggcc aggtcgccat
     2581 cgaagccctc gccagtcagg agttcgacct cgtcttcacc gacgtggtga tgccgcgcgc
     2641 tacgggcatc caggtgctgg aggctgccct gagccagaag cgcccgcccc gcgttgtcgt
     2701 ggtctccggc aaggagatcg cccactcgga acgcgacttc ctcgactatg gcgcctacgc
     2761 gtacttccgc aagccctatc ggctggcgga catcctgtcc gtgacccaac gcgtcgccgc
     2821 cgagctggcc gctgtctgcg cgaggtagcc gtcgcatcta cccgcgcttg cacggcgcgc
     2881 tgtggggtgt cacgccctcc gggggctcag cgaacgccga gttcccagcc ccagaggcac
     2941 agcgcgggcc cgcccgttgc gcgtgctcgg ggcgggcagg gcgacggagg cgtcgtcagc
     3001 tttcggcagc cgccgttact cggcgttcgc ccgctccagc ctatcggcgg cgcgaaggac
     3061 tcgctcgcgg atgcggctga cggcttcggg gtcgggcagg agctccgacg agaagcgccc
     3121 gccgttgttc gggatgctca cgagggcccg ggccgcggcc agggcagcgt cggcatcggc
     3181 ggtcttccgg ccggcggcgc gggcctcgtc gacccgctgc tgcaggatgc gcagtgcctc
     3241 gtagtcctcc atcccctcgc gagcctgctc aagccggatg gagctgaccg gccccttcac
     3301 gccgacggca tcgcccgggt aggtcagata gccgtcgccg ttcgggtagc ggatgtagta
     3361 gtactccttc ccctcatcgc tctgacgaat gtactggtgc cagccgcgct cccacgggtt
     3421 gtacgtccac cacgaaacac cccagaactc gtaggccgcg tggtcgtagc ggaagcagaa
     3481 gtacggcagc aggcgctcga tagcacagta gggggtatcg atacacatct gcccatcggt
     3541 ggtgaaccac agggtctcgc ccgcggcgcg gcggcgcgcc atctcatcga ccgggaaggt
     3601 cccctgaggg ccggtgcccc atatgtcgag cgcgcccacg agccgctggc tgaagcgcca
     3661 ggtgctcgag tagatgggca cggcagggtc ggcttcgtgc gccagcgctg cgatcttctg
     3721 caggttggcg gctacgtcgt tgtcgccgtc catgaagggc tcatcggaga tgtagtagac
     3781 gagcctgtcg agccagccac gttcgcgcag gtgatcggag agcaggcggt aggcctgctt
     3841 gaaggccgcc tcgtactccg gcgtgaaggg ctcgaggccg aagatggctt tggggggata
     3901 cgcccaaagc catcttcggc ctcgagccct tcacgccgga gtacgaggcg gaagatg
//
LOCUS       DAOSPL010000236         3948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_23036_length_3948_cov_3.891343, whole genome shotgun sequence.
ACCESSION   DAOSPL010000236 DAOSPL010000000
VERSION     DAOSPL010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23036_length_3948_cov_3.891343"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2049
                     /gene="fusA"
                     /locus_tag="PLQ54_05715"
     CDS             <1..2049
                     /gene="fusA"
                     /locus_tag="PLQ54_05715"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOS92788.1"
                     /translation="ALVSYANAGKTSLVEALLFNAKAVERLGKVDAGTATADFDSQER
                     DRGVSIGAALCAFEHAGVKINALDTPGYADFIAEAISCLRVADGAVVLVDATAGVEVG
                     TERTWRYCDHYELPRLIVINKLDKESANFADCVSAIRQRLSNRAVPIHVPIGSAASFQ
                     GFVDLVEMKAFTAAGEMGPATAGEIPADMAGAIEEARMALVEGVAESDDTLLEKYLDT
                     GELSADEVRNALLDAVRSGGLVPILCASATRNIGARHVADAIANLLPPPRTKTLEGRT
                     PDGDGVVTREPSSDGPFCGYVFKTFIDEYVGRISLLRVYDGQARPDSPVTNTVSGQKE
                     RLGQILTLRGRNQEVLPHGDLGDIVAVGRLGNTLTNQTLCAPNQLMVLPVTELPEPMF
                     SASLQAKSREDQEKIAQVLARLTEEDVALRFFRDEETEEAIIEGMGQLHLEIAIRRLR
                     ERGVEAELGKPKIPYRETVRRKAEHTYRHKKQTGGRGQFAQVSLRIEPTERGEGFQFV
                     NEIKGAVISGGYIPGVEKGVREGMGRGILAGFPVVDVRVTVFDGKEHPVDSSELAFRI
                     AASRCFAEGARQCDPILLEPVVAVDVVTPDDSMGEIISDLNGKRGRILGMEPMGGNQI
                     VHAHVPLAELSSYGAELHSLTGGRAYYSVAPSHYEEVPPHLVDAIVREYGRKVEEEEE
                     "
     gene            2106..2474
                     /gene="rplN"
                     /locus_tag="PLQ54_05720"
     CDS             2106..2474
                     /gene="rplN"
                     /locus_tag="PLQ54_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOS92789.1"
                     /translation="MIQPMTRLTVADNTGAREVLVIRVLGKGNTHSGGIGDVFVAAVK
                     EATPTGQVREHQVVKAVVVRTKNRIRRPDGSYIRFDDNAAVIIDNNGDPRGTRIFGPV
                     ARELRDANFMKVISLAPEVW"
     gene            2494..2850
                     /gene="rplX"
                     /locus_tag="PLQ54_05725"
     CDS             2494..2850
                     /gene="rplX"
                     /locus_tag="PLQ54_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012939251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOS92790.1"
                     /translation="MAEATKLHVKKGDTVIVLSGDDKYRESDDRKRGKGRVLSADPKT
                     GRVVVEGVNIVKKHQKPRGRKPGGIVQKPAPIHASNVMLVCPNCQLPSRMSHIRGADG
                     RIQRVCKKCKKSVDQR"
     gene            2902..3447
                     /gene="rplE"
                     /locus_tag="PLQ54_05730"
     CDS             2902..3447
                     /gene="rplE"
                     /locus_tag="PLQ54_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOS92791.1"
                     /translation="MTPRFKERYKAEIVPRLMEQFGYRNVMEVPRPVKVVLNMGVTGA
                     REDRKLLDDAMRELGRIAGQAPSITRATKSVANFKIRKGMAIGCRVTLRGDRMYEFLD
                     RLITIALPRIRDFRGLNPSSFDGRGSYSLGVTEQTIFPEIHYDEIERARGMDITIVTS
                     AKTDAEARALLLELGLPLRTS"
     gene            3465..3650
                     /locus_tag="PLQ54_05735"
     CDS             3465..3650
                     /locus_tag="PLQ54_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004268593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOS92792.1"
                     /translation="MAKSALKQKCKRPPKFKVRAYTRCQVCGRPRAVLRKYGLCRICL
                     RFLALEGLIPGVTKSSW"
     gene            3730..>3948
                     /gene="rpsH"
                     /locus_tag="PLQ54_05740"
     CDS             3730..>3948
                     /gene="rpsH"
                     /locus_tag="PLQ54_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002818466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOS92793.1"
                     /translation="MSMSDPIADMLTRVRNASFARHEHVEVPASRLKIEIAKILRNEG
                     FIRNYRVLTNNKQNTIKIRLKFGPNRERV"
BASE COUNT          727 a         1204 c         1393 g          624 t
ORIGIN      
        1 gcgctggtgt cctacgccaa cgcgggaaag acgtcgctgg tggaggccct gctgttcaac
       61 gccaaggccg tggagcgcct cggcaaagtg gacgccggga cggctaccgc ggattttgac
      121 agccaagagc gggaccgcgg tgtgtcgatc ggcgccgcgc tgtgcgcgtt cgagcacgcg
      181 ggtgtgaaga tcaacgcgct ggataccccg ggatatgcgg acttcatcgc cgaggccatc
      241 agctgcctgc gcgtggccga tggtgcggtg gtcctggtgg atgccaccgc cggcgtcgag
      301 gtgggcaccg agcgcacgtg gcgctattgc gaccactatg aactacctcg cctcatcgtg
      361 atcaacaaac tcgataagga gagcgcgaac ttcgccgact gcgtttcggc catccggcag
      421 cgtctctcga accgcgcagt gcccattcat gtgcccatcg gctccgcggc cagcttccag
      481 ggcttcgttg acctggtcga gatgaaggcg ttcacggccg cgggtgagat gggccccgcc
      541 accgccggag agatccccgc cgacatggcc ggcgccatcg aggaagcgcg gatggcactg
      601 gtggagggcg tggccgagag cgacgacacg ctgctcgaaa agtacctgga caccggcgag
      661 ctgtcggccg atgaggtccg caacgcgttg ctggacgcgg tccggtcggg cgggctcgtg
      721 cccatcctgt gcgcctcggc gacccggaac atcggggccc ggcacgtggc cgacgccatc
      781 gcgaatctgc tgcccccgcc gcggaccaag accctcgaag gcagaacgcc cgatggcgat
      841 ggcgtggtga ctcgcgagcc cagcagtgac gggcccttct gcggctacgt gttcaagacg
      901 ttcatcgacg agtacgtggg acgcatctcg ctgctgcggg tctacgacgg ccaggctcgg
      961 cctgactcgc ccgtcaccaa caccgtgagc ggccagaagg agcgactagg acagatcctg
     1021 acgctccggg gccggaacca ggaggtgctg ccgcacggcg acctgggtga catcgttgcc
     1081 gtgggacgcc tcgggaacac cctcacgaac cagacgctgt gcgcgcccaa ccagctcatg
     1141 gtgttgccgg tcaccgaact ccccgaaccc atgttctcgg cgtcattgca ggcgaaatcg
     1201 cgcgaggatc aggagaagat cgcccaggtg ctcgcccgcc tgaccgagga ggatgtggcc
     1261 ctccggttct tccgcgatga ggagaccgag gaggccatca tcgaggggat gggacagctg
     1321 cacctcgaga tcgccatccg ccgcctgcgc gagcggggcg tcgaggccga gctgggcaag
     1381 cccaagatcc cgtaccgcga gacggtccgc cgcaaggccg aacacaccta tcggcacaag
     1441 aagcagaccg gtgggcgggg ccagttcgcg caggtctccc tgcgcatcga gcccacggag
     1501 cgcggcgagg gcttccagtt cgtgaatgag atcaaggggg ccgtcatctc cggcggctac
     1561 atccccgggg ttgagaaggg tgtgcgcgag ggcatggggc gcggcatcct ggcgggcttc
     1621 cccgtggtcg atgttcgcgt caccgtcttc gacggcaagg agcacccggt ggactcgtct
     1681 gagttggcgt tccgcatcgc ggcgtcgcga tgcttcgccg agggtgcccg ccagtgtgat
     1741 cccattctgc tggagccggt ggtggcggtc gatgtggtga cgcccgatga cagcatgggg
     1801 gagatcatca gcgacctgaa cggcaagcgg ggccggattc tggggatgga gcccatgggc
     1861 gggaaccaga tcgtgcacgc ccacgtcccg ctggcagagc tgtcgtccta cggcgccgaa
     1921 ctgcattcgc tgaccggcgg ccgggcgtac tactcggtgg cgccctcgca ttacgaggag
     1981 gtcccgccgc acttggtgga cgccatcgtt cgcgagtatg gccgcaaggt ggaagaggaa
     2041 gaggagtagg ccaggcggcc tgctccgcgg aggccgctgg gccccccggt tgaggaggca
     2101 cgacgatgat ccagccgatg actcggttga cggtggcgga caacaccggc gcccgggagg
     2161 tcctggtgat ccgggtcttg ggtaagggga acacgcactc gggcggcatt ggtgacgtgt
     2221 tcgtggctgc ggtgaaggaa gccacgccga cgggccaggt tcgggagcac caggtcgtca
     2281 aggccgtggt ggtccggacc aagaaccgga tccgacggcc cgatggttcg tacatccgct
     2341 tcgatgacaa cgccgcggtc atcatcgaca acaacgggga tccgaggggc acacgcatct
     2401 tcggcccagt ggcgcgcgag cttcgggatg ccaacttcat gaaggtcatc tcgctggctc
     2461 cggaagtctg gtagggcgaa ggtggtggga ccagtggccg aagcgaccaa gctgcacgtg
     2521 aaaaagggcg acaccgtcat cgtcttgtcg ggcgatgaca agtaccgcga gagcgacgac
     2581 cggaagcggg gcaagggccg tgtgctcagc gccgatccga agacggggcg cgttgtcgtc
     2641 gagggcgtga acatcgtcaa gaagcaccag aagcctcggg gccgcaagcc gggaggcatt
     2701 gtgcagaaac ccgcaccgat tcatgcatcc aacgtcatgc tggtgtgccc gaactgccaa
     2761 ctgccctcgc ggatgtccca cattcgtggg gccgacggcc ggatccagcg cgtgtgcaag
     2821 aagtgcaaga agagcgtgga ccagcgatag cctgtcgcgc gtggggatcg ggtaagggtt
     2881 gccccacggg gagtggaaga catgacaccg aggttcaaag agcgctacaa ggccgagatc
     2941 gtgccgcgac tcatggagca gtttgggtac cgcaacgtca tggaggtacc ccggccggtc
     3001 aaagtggttc tgaacatggg tgtgaccggc gcacgcgagg accgcaagct cctcgacgac
     3061 gccatgcggg agctgggccg catcgccgga caggcgccct cgatcacacg ggcgaccaag
     3121 tcggtggcca acttcaagat ccgcaaaggg atggccatcg ggtgccgcgt gacgctgcgg
     3181 ggcgaccgga tgtatgagtt cctggaccgg ctcatcacca tcgcccttcc gcggatccgg
     3241 gacttccggg ggctgaaccc gtcgtccttt gacggccggg ggagttacag cctgggcgtg
     3301 accgagcaga cgatattccc cgagatccac tacgatgaga tcgagagggc tcggggtatg
     3361 gatatcacca tcgtgacgag cgcgaagact gacgcggagg cgcgggccct gctgctggaa
     3421 ctgggcctgc cgctgcggac gagctaggga aggataacca cgggatggcc aagtcagccc
     3481 tcaagcagaa gtgcaagcgc cccccgaagt tcaaggtacg cgcgtacacg cggtgccagg
     3541 tgtgtggccg tccgcgggcc gtgctccgga agtacggcct gtgtaggatc tgcctgcgtt
     3601 tcctggccct cgagggcttg attccgggcg tgaccaagtc gtcctggtag gcatggatgc
     3661 gtgccttggg gccacgccgg tgtcgtcgtg cggcatgtcc gcggtcgtcc tcataaggag
     3721 ggtgcttcaa tgagcatgag cgatccgatt gccgatatgc tcactcgcgt gcgcaacgcg
     3781 agcttcgcgc gtcacgagca cgtcgaggtt ccggcctccc ggctgaagat cgagatcgcc
     3841 aagatccttc ggaacgaggg cttcatccgg aactatcgtg tgctgacgaa caacaagcag
     3901 aacacgatca agatccggct gaagttcgga ccgaaccggg agcgcgtg
//
LOCUS       DAOSPL010000237         3948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_23038_length_3948_cov_3.204726, whole genome shotgun sequence.
ACCESSION   DAOSPL010000237 DAOSPL010000000
VERSION     DAOSPL010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23038_length_3948_cov_3.204726"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3461)
                     /locus_tag="PLQ54_05745"
     CDS             complement(<1..3461)
                     /locus_tag="PLQ54_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOS92794.1"
                     /translation="MEAPKGSEGTRSRGPLLGAAAQGEAWLQSMIDSLPYQVYLKDAQ
                     GRFLMVNRAMVEAIGKPPGDIIGRNLSDLLPDRDAASRCQQEARILETGVPLEDCVYS
                     HTDAQGQQHWYSETEARVPLPHGGFGLVGVTRELNPQQQAAERLRIQKELAVRLLSAE
                     TIEAALSDALRAAIEGTGADGGAAYLADPASESLSLYEAVSLSPPPRLPLAGFSTLGE
                     GQLRTIHMATSQLDAYPEVRDALAAQGVRCFAAAPLGQPDHLLASLHVASRAFDRFPP
                     EAREHLEAVVALAGQALARVAAQCSLRESESRYRTMLQEASDALFVLALTDAGPGQFI
                     DANDQAVRLLGYSRAELLSMSITDVDVPEQLAHVPGIIARLRDTGSAVFETEHLTKDG
                     RRVPVEVSARLIDLQGQTVVLAAVRDVTDRHRAEAALRESQRRLMDIIDSLPDATFVL
                     DASGRVTAWNRAIQDMTGVPAEAVVGQGGYQHAIPFYGEPRPSLADLLLQGLEPAPDR
                     YRMISREGGVVVAEATVPMTGRGMRDLWARAVLLHDDRGAVVGAIETIRDVTERRRAE
                     EALRDSERQLADIIDFLPDATFVIDTHGRVVAWNRAIEEMTGIPAHEILGKGDHEYTV
                     PFYGYRRKCLADLVLRYDDVAEEYYPGIRREGGALVAEIPSVCPRGRDMAVWAKATPL
                     RDRDGRITGAIEAIRDITERRRAEEALRAREEQYRLIVETSNEGILPTDPRGVIRFAN
                     PRMAEIVGHQLAQLIGRNAAEFMDPEEAADHARRMAERATGVSAQYERRLRHSDGHEV
                     WVAISSTPIIDDDGRFTGSFAMVTDITERKRAERERELLETQLADARKLESIGQLAGG
                     VAHDLNNMLAPILGYAELLAGDPAADPRQQSALQEIIKAAQRARDLVRQLLAFARRQT
                     LNVRPIDLNKVVVDLEPMLRRTIRENVDIALDLDPALPSVQGDAGQIEQIILNLAVNA
                     QDAMPDGGVLAIGTSAAVVEGAGPDCPAGIAPGRYALLTVRDNGVGMDEATLSRAFEP
                     FFTTKATGRGTGLGLSTVYGIATQHGGHVAAQSEVGKGTVIRVYLPQAAEAPESNVSG
                     TPEGPAAGNETVMVVEDQAQVRDLVGAMLEQSGYTVLSAADGAEAVERSEGWKGPIHL
                     LVTDVIMR"
     gene            3751..>3948
                     /locus_tag="PLQ54_05750"
     CDS             3751..>3948
                     /locus_tag="PLQ54_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92795.1"
                     /translation="MSDQPESPGALIANRYRLIRLIGRGSFGSVYEAREYLVGKYVGR
                     VAVKLIPPPKNPDLVAEHQERV"
BASE COUNT          601 a         1293 c         1386 g          668 t
ORIGIN      
        1 cgcatgatca catccgtcac gaggagatga atggggcctt tccagccctc actgcgctca
       61 acggcctctg ctccatcggc ggcggagagc acggtgtacc cgctctgctc gagcatcgcg
      121 ccaaccaggt cacgcacttg ggcttggtcc tccacgacca tcacggtctc attgcccgca
      181 gcagggccct cgggcgtccc ggagacgttg gactcgggtg cctcagcggc ctgagggaga
      241 tagacgcgga taacggtgcc cttgccgacc tcactctggg ccgcgacatg gccgccgtgc
      301 tgggtggcga tgccgtagac cgtcgacagt ccgagaccgg tgccgcggcc ggtggccttg
      361 gtcgtgaaga acggctcgaa ggcccggctg agagtggctt cgtccatgcc cacgccgttg
      421 tcgcgcacgg tcagcaaggc gtatcggccg ggcgcgatgc cggctgggca gtcggggccg
      481 gccccttcga cgacggccgc tgatgtcccg atggcgagaa cgcccccgtc cggcatggca
      541 tcctgggcgt tcacggcgag gttgaggatg atttgctcga tctggccggc gtcgccctgg
      601 actgacggca gggccggatc gaggtccaga gcgatgtcca cattctcccg gatggtccgg
      661 cggagcatgg gttccaggtc gaccactacc ttgttgaggt cgatggggcg cacgttgagg
      721 gtctggcgcc gggcgaaggc gagaagctgc cgcacgagat cgcgggcccg ctgcgcggcc
      781 ttgatgatct cctgcagggc cgactgctgg cgcgggtctg ccgccgggtc gcctgccagt
      841 agctccgcat agcccaggat cggcgccagc atgttgttca ggtcgtgggc caccccgccc
      901 gccagctgcc caatggattc gagcttgcga gcgtcggcca gctgtgtctc gagcaactcg
      961 cgctcgcgct cggcgcgctt ccgctcggtg atgtcggtga ccatggcgaa cgacccagtg
     1021 aaccgcccat catcgtcgat gatcggggtg gacgagatcg cgacccagac ctcgtggccg
     1081 tcactgtggc ggagacgccg ctcgtactgg gcagagacgc cggttgcgcg ttcagccatc
     1141 cggcgcgcgt gatccgcggc ctcttcgggg tccatgaact cggccgcgtt acggccgatc
     1201 aactgcgcga gctgatgacc cacgatctcg gccattcgcg ggttggcgaa gcgaatgaca
     1261 ccccgggggt cggtgggcag gatgccctca ttggatgtct cgacgatgag gcggtactgc
     1321 tcctctcgcg cgcggagtgc ctcctcggcc cggcgacgtt cggtgatgtc gcgaatcgcc
     1381 tcgatcgccc ccgtgatgcg accgtccctg tcgcgaagcg gtgtggcctt cgcccagact
     1441 gccatgtcgc ggccccgggg acacaccgat ggtatctcag cgacgagcgc gcccccctca
     1501 cgccggatcc cggggtagta ctcctcggcg acgtcatcgt accggagcac aagatcggca
     1561 aggcacttgc ggcggtagcc atagaagggc acggtgtact cgtggtcgcc cttgcccagg
     1621 atctcgtgtg cggggatgcc cgtcatttcc tcgatggccc ggttccatgc gaccacgcgg
     1681 ccgtgggtgt cgatgacgaa ggtggcgtcg gggaggaagt cgatgatgtc ggccaactgg
     1741 cgttcgctgt cgcgcagggc ctcctcggcg cggcgccgct cggtgacatc gcggatggtc
     1801 tcgatcgcgc cgaccacagc accacgatcg tcatggagca gaacggccct ggcccagagg
     1861 tcgcgcatcc ccctaccggt catcggcaca gtggcctcgg cgaccacgac gccgccttcg
     1921 cgactgatca tccggtaccg gtcgggggcg ggctcgagtc cctgaaggag gagatcggcg
     1981 agagacggtc tgggctcgcc gtagaaaggg atggcgtgct gataaccacc ttggcccaca
     2041 accgcctccg cggggacacc ggtcatgtcc tggatggccc tgttccaggc ggtcacacgg
     2101 ccggacgcat cgagcacgaa ggtcgcatcg ggaagggagt cgataatgtc catgagccgc
     2161 cgctggctct cgcggagggc cgcttcggcc cggtgccggt cggtgacatc gcgcaccgcg
     2221 gccagaacga cggtctggcc ctgcaggtcg atcaggcgtg cgctcacctc gacgggtaca
     2281 cgtcggccgt ctttcgtgag gtgctcggtc tcgaacaccg cactcccggt gtcgcggagt
     2341 ctggcgatga tgccgggcac atgggccaac tgctccggga cgtcgacatc cgtgatgctc
     2401 atcgacagca gctccgcgcg ggagtatccc aggagtcgga ctgcctggtc gttcgcgtcg
     2461 atgaactggc ccgggcccgc gtcggtcaat gccaacacga acagggcgtc gctggcctct
     2521 tgcagcatgg tccggtagcg cgactcgctt tcgcggagcg agcactgggc cgccacgcgg
     2581 gccagagcct gccctgcgag ggcgaccacg gcctccagat gctcccgcgc ctccggaggg
     2641 aagcggtcga atgcccgcga tgccacgtgg agtgacgcga gaaggtggtc gggctgtccc
     2701 aggggcgctg cggcgaagca acggacgccc tgggcggcca atgcgtcacg gacctccggg
     2761 tatgcgtcca gttggctggt ggccatgtgg atcgtccgca gttggccctc accgagagta
     2821 gagaagccgg ccaagggcaa gcgcggtggc ggagacaggc tgacggcctc gtagagagac
     2881 agcgactcag aggccggatc ggccaagtat gccgctccac cgtcggctcc ggttccctcg
     2941 atggcggctc ggagcgcatc ggagagagcg gcctcgatgg tctcggccga cagaaggcgg
     3001 acggccagct ccttctggat gcgcagacgt tcggccgctt gctgttgggg gttgagctcg
     3061 cgggtcaccc ctacgaggcc gaatccgcca tggggcaggg ggacccgggc ttccgtctcg
     3121 gagtaccagt gctgttggcc ttgggcgtcg gtgtgagagt agacgcagtc ctcgaggggc
     3181 acaccggtct cgagtatccg tgcctcttgc tggcaccggg aggcagcatc acggtcaggc
     3241 agcagatcgg aaaggttccg gccgatgatg tcgcccggcg gcttaccgat cgcctctacc
     3301 attgcccggt tgaccatgag aaaccggcct tgggcgtcct tgaggtagac ctggtagggc
     3361 aggctgtcga tcatgctttg gagccatgcc tcgccctgcg cggccgcccc gagaagcggg
     3421 ccgcggctgc gtgttccctc gctccccttc ggtgcctcca cggccacaga cctcccggca
     3481 ccccatccgc ggcgctcccg ccgacccggc tggcgggcac tcttgtgtta gcgcttgcag
     3541 acgtagtctt tcctcggtgg cttcgcatct cctgccagtg ccggcggagc acccggtgcc
     3601 cctggagcca cggcccgggg ctgagcctgg agtcgccgag gctgcggtct ggtccctcgc
     3661 caccaatggg tggcggggcg agcccggctg caggttgccg ggtggtggct tgagaaggga
     3721 cgcccgacac cacaaagggg acgggcaagc atgtcggatc agccggaatc tcctggcgca
     3781 ctgatcgcca atcgctaccg tctcatacgg ctgatcggtc ggggcagctt cgggtcggtg
     3841 tatgaagcgc gcgagtacct cgtggggaag tacgtaggtc gcgtggccgt gaagctcatc
     3901 ccgccgccca agaacccgga cctggtggcg gagcatcagg agcgcgtc
//
LOCUS       DAOSPL010000238         3920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_23234_length_3920_cov_3.374386, whole genome shotgun sequence.
ACCESSION   DAOSPL010000238 DAOSPL010000000
VERSION     DAOSPL010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23234_length_3920_cov_3.374386"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1491
                     /locus_tag="PLQ54_05755"
     CDS             <1..1491
                     /locus_tag="PLQ54_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92796.1"
                     /translation="AAVRQAWPAFLGGLETLAGAAGVAGRRIVVHNPLPWDRGGSVGV
                     PWPWPVPSSVRTPGGPRTPVEYRGGGLFFEAPRVPAMGYVTYVPDPVPAEPETLSRTD
                     AETGVIESPYFRATLDRRRGAVASLIDKRTGRELADQAAPFGLGQLLHERFCRSQTEG
                     FREAYTRGGFSWTDEFGKPAMPVDEAAPYEALSPTDCTLEFAQHIGSVEATLRSESGH
                     GLPYVVRLRVSLPLHRPSVELLLTLEGKPADSWPEAGWMCLPLNVDRPQFRLGRVGSI
                     VDPARDLVPGSNHHLFWLQTGLTVTDPSGRGVGLCPIDHPLVALGEPGCYRYDREYTP
                     ARPYVFVNLFNNQWSTNFRLWNEGTWRSGITLWAVDGHDPDSLVTPALEALLSLAAAL
                     AEGPPGPLPPERRGLELSLPGVRVAAFGPNPDGEGTLLRLWELAGRGGPCRVRLPEGS
                     RTRVVQPVDLRGRPQGEPIRARGGQFSVQIRPFAPVSLLLDGQTQH"
     gene            1507..1920
                     /locus_tag="PLQ54_05760"
     CDS             1507..1920
                     /locus_tag="PLQ54_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOS92797.1"
                     /translation="MKSATEYLWFETRRQREYLNITPQVEEVVRKSGIQEGMVLVSAM
                     HITAGVYVNDAESGLIEDIDAWLEGLAPYREGYRHHRTGETNGDAHLKSLLIHHEVIV
                     PITGGRLDLGPWQQVYYAEFDGRRRKRVVIKAMGE"
     gene            1936..2403
                     /locus_tag="PLQ54_05765"
     CDS             1936..2403
                     /locus_tag="PLQ54_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS92798.1"
                     /translation="MQIRTYRKGDLPVLRDLTIATFGGASIDRNIEERFGLLNGTDWQ
                     WRKARHIDADVAANPAGVFVMEDEGAIIGFITTRVDPTTKLGWIPNMAVAPGHQGKGI
                     GRKLLAHAIAYFRDQGMTHAKIETLAQNAVGCHLYPSVGFEEVARQVHFVMAL"
     gene            2649..3410
                     /locus_tag="PLQ54_05770"
     CDS             2649..3410
                     /locus_tag="PLQ54_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOS92799.1"
                     /translation="MRSRCRGFTLIELLVVIAIIAILAAILFPVFAKAREKARQASCQ
                     SNLKQIGLALMQYAGDYDDCYPVGRTWCNYANAAQNLQYFVQLQPYCKNWQVFACPSC
                     RANNCSNGSIPHHAVNAMISAGYAPSTFSLSYGYSEAMWNAWRPNGDYGINCGGFPTN
                     RHKMANIPTPATAPIIADCYGLLNNGARIGWANVCAAACNADRQVDGNTRHNGGSNVC
                     FGDGHVKFFNARACVTNWDTGQWQAGCKWWGNGRD"
     gene            3723..>3920
                     /locus_tag="PLQ54_05775"
     CDS             3723..>3920
                     /locus_tag="PLQ54_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS92800.1"
                     /translation="MRSRRRGFTLIELLVVIAIIAILAAILFPVFAKAREKARQASCQ
                     SNLKQIGLALMQYAGDYDDCYP"
BASE COUNT          679 a         1271 c         1320 g          650 t
ORIGIN      
        1 gccgccgtcc gccaggcctg gcccgccttc ctcggcggtc tggagacgct ggccggtgcc
       61 gccggagtcg cggggcgacg cattgtggtg cacaacccgc taccgtggga ccgaggcggg
      121 tcggtgggtg ttccgtggcc ctggccggta cccagcagcg tccgcacgcc cggggggccg
      181 cgcaccccgg tcgagtaccg cggagggggc ctgttcttcg aggccccgcg agttcccgcc
      241 atgggctacg tcacctatgt gcccgacccg gtgcccgcgg agcccgagac cctgtcgcgc
      301 accgatgctg agaccggggt catcgagagt ccttacttcc gggccacgct cgaccggcgc
      361 cgtggcgccg tggcctcgct catcgacaag cgcaccggcc gcgagttggc ggaccaggcg
      421 gcacccttcg ggctcgggca gcttctgcac gagcggttct gccgaagtca gaccgagggg
      481 ttccgcgaag cctacacccg cggcggcttc agctggaccg acgagttcgg caagccggcg
      541 atgcccgtgg atgaggcggc accctacgag gcgctgtcgc ccaccgactg caccctggag
      601 ttcgcacagc acatcggctc ggtcgaggcg acgctccggt cggagtccgg ccatggcctg
      661 ccgtacgtgg tgaggctgcg cgtgtccttg cccctgcacc gcccatcggt tgagctgctc
      721 ctcacgctgg aaggcaagcc ggccgactct tggcccgagg cgggatggat gtgcctcccg
      781 ctcaacgtcg accggcccca gttccgcctc gggcgcgtcg ggtccatcgt cgacccggcg
      841 cgtgatctgg tgcctggatc gaaccaccac ctgttctggc tccaaacggg cctgaccgtg
      901 acagatccgt cgggcagggg tgtgggactg tgtcccatcg accatccact ggtggcactg
      961 ggcgagcccg ggtgctatcg gtacgaccgc gagtacactc cagccaggcc gtacgtcttc
     1021 gtgaacctgt tcaacaacca gtggagcacc aacttccgcc tgtggaacga gggaacgtgg
     1081 cggtccggca tcacgctctg ggccgtcgat ggccacgacc ccgattcact ggtgacccct
     1141 gcactcgagg cgttgctctc cctggcggcg gcgttggcag agggcccgcc aggcccgctg
     1201 ccgcccgaga ggcgtggcct cgagctatcg ctgccaggcg tccgggttgc cgccttcggc
     1261 cccaaccccg atggtgaggg caccctgctc cgcctgtggg agctggcggg gcgcggaggc
     1321 ccatgccggg tccgcctacc cgaggggagc cggacgcgtg tcgtccagcc ggtcgatctg
     1381 cgtgggcggc cgcaagggga gcccatacgc gcgcgggggg gccagttctc ggtccagatc
     1441 cggccgttcg cgcccgtgag cctgctgctc gacggccaaa ctcagcactg acgggaggag
     1501 tgacccgtga agtcagctac cgagtacctg tggttcgaga cccgcaggca acgcgagtac
     1561 ctgaacatca cgccccaggt cgaggaggtg gtgcggaaga gcggcatcca ggagggcatg
     1621 gttctggtct cggccatgca catcacggcg ggggtctacg tcaacgacgc ggagagcgga
     1681 ctcatcgagg acattgacgc gtggctcgag ggtctggccc cataccgcga gggctaccgg
     1741 caccaccgca cgggcgagac caatggcgac gcccacctca agagcctgct gatccaccac
     1801 gaggtgatcg ttcccatcac ggggggcagg ctcgacctcg ggccctggca gcaggtctat
     1861 tacgccgagt tcgacggccg ccgccgaaag cgcgtcgtca tcaaggccat gggcgagtag
     1921 ggaggcagtg taccgatgca gatccgcacg taccgtaagg gtgacctgcc tgtgcttcgc
     1981 gacctgacca tcgccacctt cggaggcgcg tcgatcgacc gcaacatcga ggaacgcttc
     2041 gggctgctca acggcactga ctggcagtgg cgcaaggcca gacatatcga tgccgacgtg
     2101 gccgccaatc cggcgggcgt cttcgtgatg gaggacgagg gggccatcat cggctttatc
     2161 accacccggg tggaccccac gaccaagctc ggttggatcc ccaacatggc agtcgcgcca
     2221 gggcaccagg gcaagggcat cgggcggaag ctcctggcgc acgccatcgc gtacttccgc
     2281 gaccagggca tgacccatgc caagatcgag accctcgcgc agaatgcggt cggctgccac
     2341 ctctacccat cggtggggtt tgaggaagtc gcccggcagg ttcacttcgt catggctcta
     2401 taagaatcca tagcagtcta cagaggtatc ctctcgaacc gggtggaata gagccccgag
     2461 ggggtattgc ccggggtggg gctgtgtccc caaaaaatca tagtaaccat agctggtcac
     2521 agaggaatag ccctcgatcc ggcgaatggg cagtcgatca cggggcgggc tgtgcccagc
     2581 ccgtgggccg aaacgggagt gcttggtgcg cgccgcaccc ccgagtgtcg agaggagagt
     2641 gggttgcatt gcggtcgagg tgtagaggct tcaccctgat cgagctgctt gtggttattg
     2701 cgatcattgc catcctagca gccatcctgt tcccggtctt tgcgaaggcg cgagagaagg
     2761 cgcgccaggc ctcctgtcag agcaacctca agcagatcgg cttggctctg atgcagtatg
     2821 cgggtgatta tgacgactgc tacccagtcg gtcgcacctg gtgcaactac gccaatgcag
     2881 cgcagaacct gcagtacttc gtccagcttc agccctactg caagaactgg caagtgttcg
     2941 cgtgccccag ctgccgggcc aacaactgct cgaatggctc cattccgcac catgccgtca
     3001 atgccatgat cagtgccggc tacgcgccga gtaccttcag tctcagctac gggtacagcg
     3061 aggccatgtg gaacgcttgg cgtcccaacg gcgactacgg catcaactgc ggtggtttcc
     3121 cgacgaatcg gcacaagatg gcgaacatcc ccacacccgc gactgcgccg atcatcgccg
     3181 actgctacgg gctcctcaac aacggcgccc gcatcggctg ggccaatgtc tgcgccgccg
     3241 cgtgcaacgc ggaccggcaa gtcgatggca acacgcgtca caacggtggg tccaatgtgt
     3301 gctttggtga tgggcacgtc aagttcttca atgcccgtgc ctgcgtgacg aactgggaca
     3361 cgggccagtg gcaggcagga tgcaagtggt ggggtaacgg gcgcgactag aagcggctga
     3421 ggcgcacagc accgggggtg gcggctcgaa ccggcgtcca cccctggtgc tgagattcga
     3481 tgtgggggga cggaccgacc cgcagaggtg cggggatctg agcggagcgg gagtcccggc
     3541 agcggctcgg ctgtcgggaa tggcttcgct ttggaaaaaa ggcgaaggaa tccggtaggg
     3601 atgccgggaa ttagccgagg tactccagtg ggtgcgctgg gggacccggc tgcgcttatg
     3661 cgccacaggc cgcacacccc cagccgcgat ccacccggag atcgaaggga gagtgggttg
     3721 tattgcggtc gaggcgtaga ggcttcaccc tgatcgagct gcttgtggtc atcgcgatca
     3781 ttgccatcct agcagccatc ttgttcccgg tcttcgcgaa ggcacgtgag aaggcccgtc
     3841 aggcttcctg ccagagcaat ctcaaacaga tcggcctcgc tctgatgcag tacgccggcg
     3901 attacgacga ctgctacccc
//
LOCUS       DAOSPL010000239         3917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_23261_length_3917_cov_3.212843, whole genome shotgun sequence.
ACCESSION   DAOSPL010000239 DAOSPL010000000
VERSION     DAOSPL010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23261_length_3917_cov_3.212843"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1769)
                     /locus_tag="PLQ54_05780"
     CDS             complement(<1..1769)
                     /locus_tag="PLQ54_05780"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOS92801.1"
                     /translation="MAIHKVSMDLAGRTLEIETGRMAHQAYGAVTVRAGDSMVLVTAT
                     GSDTVRDGIDFFPLTCDYEERLYAAGKIPGSFPRREGRPSDSAVLTCRLMDRGLRPLF
                     PKGYRNDVQIIAMPMSSDRQNQVDILCMIGAAAALAISGLPVQHTLGSIRIGRIDGEP
                     VVYPTHDECGEAELDLVVSATREAIVMIEVGALEIPEAELVECMKLAHSECIRIIDLI
                     EELKAKVNPEPRPYQVYLPSDEVYEAAQGYQRELIDALSLPDKAARRREYDAVLSRAK
                     EELAARFEDKEPWEFSDALDSVTKSGVRALILSGKRLDGRAPDEIRPLAGEVGLAPRA
                     HGTGLFSRGDTQTLCTVTLGMLAEAQKLDTLSLEDSKTFMHQYNMPPFANGEARPLRG
                     PNRRAIGHGALAERALLSVVPSTEEFPYTVRLVNEVLGSDGSTSMASVCSASLAMMDA
                     GIPIDAHVAGMAMGIVYSPDQHVILTDIQAQEDFNGDMDFKVAGTREGVTAIQMDVKC
                     LGLTTELLSEALEHARVARMKVLDVLEATIPAPRDELSPYAPRMLTLMVPVDKIGAVI
                     GPGGKTIRKIEALGVTIEIEDDGR"
     gene            complement(2011..2280)
                     /gene="rpsO"
                     /locus_tag="PLQ54_05785"
     CDS             complement(2011..2280)
                     /gene="rpsO"
                     /locus_tag="PLQ54_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOS92802.1"
                     /translation="MPIDPERKTEIISEHRRKVDDTGSPEVQIALLTERISQLTEHLK
                     VHKKDYHSRRGLLRMVGKRRRLLRYLEHADIERYRALVARLGLRR"
     gene            2633..3586
                     /locus_tag="PLQ54_05790"
     CDS             2633..3586
                     /locus_tag="PLQ54_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS92803.1"
                     /translation="MADDRARAMRFEHPEFIPLSIGFLPATWKRHRGALDELVARHPI
                     IFGEPSPVPRDYDAVGGTYAAGTHVDAWNCVWSNIAEGAEAYVSGHPLPERCMVRTFQ
                     PPEPGAGLPHGFMYLRLGYLRGWEEILMDFAEEPEELRILVDTVGRYNVGEVRRMLEN
                     PPAMAYFGDDLGMQDRLPISPTKWRTYLKPWYAEMYGLCHEAGTAVYMHSDGHMVPII
                     ADLIECGVDVLNPQIRANGLDRLVEECRGKVCVDLDLDRQLFPFCSPADIDAHVREAV
                     EKLALPEGGLWLHAECGPDVPLENIEAICQAFERYRAWHRE"
     gene            complement(3691..>3917)
                     /locus_tag="PLQ54_05795"
     CDS             complement(3691..>3917)
                     /locus_tag="PLQ54_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOS92804.1"
                     /translation="NFLWMWNELLFALLILQKDTARTLMVGVANLRGQYTTSIPLMSA
                     GLFLAALPVLIVFFVFQSQIQKGMTMGAVK"
BASE COUNT          664 a         1308 c         1253 g          692 t
ORIGIN      
        1 cggccgtcgt cctcgatctc gatggtgacc ccgagcgcct cgatcttgcg gatggtcttg
       61 ccgcccgggc cgatcacggc accgatcttg tcgaccggta ccatcagcgt gagcatgcgt
      121 ggggcgtacg gtgacagctc gtcgcgcgga gccggaatgg tggcctcgag cacatccaga
      181 actttcatcc gggccacgcg ggcatgttcg agcgcctcag aaagtaactc ggtagtcaac
      241 cccaaacact tgacatccat ctggatggcc gtgacgccct cgcgggtgcc cgccaccttg
      301 aagtccatgt ccccattgaa gtcctcctga gcctgaatgt cggtcaggat gacatgctgg
      361 tccggtgagt agacgatgcc catggccatg cccgcgacgt gggcgtcgat ggggatgccg
      421 gcatccatca tggccaggct cgcgctgcac acactggcca ttgaggtcga cccgtcagac
      481 ccgagtacct cgttgaccag ccgcacggtg tacgggaact cctcggtgct cgggaccacc
      541 gacagcaggg cccgctcggc cagagcgcca tgcccgatcg ctctccggtt cggcccacgc
      601 agcggccgtg cctcgccatt ggcaaagggg ggcatgttgt actggtgcat gaacgtcttc
      661 gagtcttcga gactgagcgt atcgagcttc tgcgcctccg cgagcatgcc cagcgtgacc
      721 gtacagagcg tctgggtgtc gcccctggag aacaggcccg ttccgtgcgc ccggggggcg
      781 agacccacct caccggccaa gggccggatc tcatcgggtg cacggccgtc gagacgcttg
      841 cccgagagaa tcagggcccg gacgcctgac ttggtgacgc tgtccagggc atcggagaac
      901 tcccagggct ccttgtcttc gaagcgagcg gcgagttcct ccttagctcg actgagcacg
      961 gcgtcgtatt cccggcggcg cgcggccttg tccggaagac tgagtgcgtc gataagctcc
     1021 ctctgatagc cctgggccgc ctcatagacc tcgtccgagg ggagatagac ctggtacggc
     1081 ctgggttccg ggttgacctt cgccttgagt tcctcgatga gatcgatgat gcggatgcac
     1141 tcgctgtggg ccagcttcat gcactcaacg agttccgcct cggggatctc cagcgcgccg
     1201 acctcgatca tgacgatggc ctcgcgtgtg gccgaaacga ccaagtcgag ttcggcctca
     1261 ccgcattcgt cgtgagtcgg gtagaccacg ggttcgccgt cgatccggcc gatacggatc
     1321 gaccccaggg tgtgctgcac cggtagtccg ctgatggcca gggccgcggc ggcgccgatc
     1381 atgcacagta tgtcgacctg gttctgccgg tccgacgaca tgggcatggc gatgatctga
     1441 acgtcgttgc ggtagccttt ggggaagagc gggcgcaggc cccggtccat cagccggcac
     1501 gtcagcacgg cgctgtcgct gggccggccc tctcgacggg ggaaactccc ggggatcttc
     1561 ccggcggcat agagccgctc ctcgtagtcg caggtcagcg ggaagaagtc gataccgtcc
     1621 cgcacggtgt cggacccggt ggcggtgacg aggaccatgg agtcgcccgc acgcacggtg
     1681 acggctccat acgcctgatg ggccatgcgt cctgtctcga tctcgagcgt acggcctgca
     1741 aggtccatgg agaccttatg aatggccact acaaaacctc ctgcggcagg gccgccagcc
     1801 tagcactgcg gggcggaagc ctgccacgac tctcgcagcc gcttccgtct cggagtacta
     1861 gggatcagcg gacccccacc cctcaaatcg cgggcggcaa agccgcccgt ccgttcacgt
     1921 cctgacgaac cgcgagccgg gaccacgctg cacaaggccc cggctctgcg ggaacatccc
     1981 cgatcacctg actccgaact cgtccagcgg ctagcgcctc aggcccagcc gcgccaccaa
     2041 tgcgcgatag cgctcgatgt ctgcgtgctc gagatagcga agcaggcggc gccgtttgcc
     2101 caccattcgc agcagaccgc gccgcgagtg gtagtccttc ttatggactt tgaggtgctc
     2161 ggtgagctgt gagatgcgtt ccgtcagcag cgcgatctgc acctcgggcg aaccggtgtc
     2221 gtcaaccttc ctcctgtgct cgctgatgat ctccgtcttg cgttcggggt cgatgggcac
     2281 gattcgtttc ctccgcttca catcggctcg cgcgagcgag cactccacac cgagacccct
     2341 cgaaccgagt gggcggccgc ggtagccgca gacccagttc cagggcgctt gaccaacggt
     2401 ggcagtgtac gccatgtgtg gctcgctgta aagcacggga caccgcgtcg cccgggtccc
     2461 ttcgtccctc catcgaaccg cgggccccat ctggcccgcg gtggaaacga gatgacccgg
     2521 gtcgcgcacc gcaggcattc ggccccctct gacccgagga ggccatcggc ccgtggccaa
     2581 acaacatact gtggcttgga ccagcacccc cttcgaaggg aacgcgtgac ctatggcaga
     2641 cgatcgcgcc agggcgatgc gctttgagca tcctgagttc attccactga gtatcggctt
     2701 cctccccgca acgtggaaac gccatcgcgg ggctctcgac gagctggtgg cccggcaccc
     2761 gattatcttc ggggagccca gtcctgtgcc gcgcgactat gatgccgtgg gcggcaccta
     2821 cgctgccggc acgcatgtcg acgcatggaa ctgcgtgtgg agcaacatcg ccgagggtgc
     2881 tgaggcctat gtcagcggcc atccactgcc ggagcgctgc atggtgagga ccttccagcc
     2941 gccagagccc ggtgccggcc tgccccacgg cttcatgtac ctccgtctcg gctacctccg
     3001 cggatgggag gagattctca tggacttcgc cgaggagccc gaggaactac ggatccttgt
     3061 cgacaccgtg ggccgctaca acgtgggcga agtccgccgg atgctggaga atccaccggc
     3121 gatggcgtat ttcggcgatg atcttgggat gcaggaccgc ctcccgatca gccccaccaa
     3181 gtggcgcact tacctcaagc cctggtatgc cgagatgtac ggcctctgcc acgaggccgg
     3241 gacggcggtc tatatgcact ccgatggcca catggtgccg atcatcgccg acctgatcga
     3301 gtgtggcgtc gatgtcctca atccgcagat ccgtgccaac gggctcgacc ggctcgtgga
     3361 ggagtgccgg ggcaaggtgt gcgtcgacct cgacctcgac cgtcagctct tccccttctg
     3421 ctcgccggcg gacattgatg cccacgtccg cgaagccgtc gagaagctgg ctctgcccga
     3481 gggcggcctc tggctgcacg ccgaatgcgg gcccgacgtc cccctcgaga acatcgaggc
     3541 catctgccag gcgttcgagc gctaccgcgc gtggcatcgg gagtaggcga gcggggcgtg
     3601 ggttccggcg gatccggtgc tgagccccca gagtgagcgt cgaccagtag cccgttgcgg
     3661 gagcggcggc cgacgccgca ccgccctgcc ctacttcacc gcccccatgg tcatgccttt
     3721 ctggatctgg gactggaaaa cgaagaacac gatcaatacc ggcagcgccg ccaggaacag
     3781 cccggcggac atcagtggga tgctggtggt gtactgacct cgcaggttgg ccacgccgac
     3841 catgagcgtt cgcgccgtgt ccttctggag aatcagcagc gcgaacagca gctcgttcca
     3901 catccagagg aagttga
//
LOCUS       DAOSPL010000240         3890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_23462_length_3890_cov_3.694915, whole genome shotgun sequence.
ACCESSION   DAOSPL010000240 DAOSPL010000000
VERSION     DAOSPL010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23462_length_3890_cov_3.694915"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..1254)
                     /locus_tag="PLQ54_05800"
     CDS             complement(250..1254)
                     /locus_tag="PLQ54_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92805.1"
                     /translation="MTPKRGPGVVLGGTLFLTALLALSGCEWITEVTGGSDDKSNERN
                     LVLPTDITATIPLGNEILFVVYEGTNAANHLERREGVFAIGSNGTRVERLTDVAGHSP
                     SFLADGRVVYWRTEGTPGLAVIAGGQATNLAINPAPSKARAVGLKGAASDRIAYVSDG
                     RLFVVNATGGDATLIAETANLPAALEDGRIAYVGPDGDVWAIAPDGTGAIRVLTPRGA
                     VRALCGMPDGKLLLATYDASKELSYIYTANVQENARASAVYGSFGLMSPLFDSELTGC
                     QVLSSGYVAFSYGLPGGSTRGLAFCQLSANMLANAVGLNKSSADDFEGLWILDGYGAL
                     "
     gene            1445..2020
                     /locus_tag="PLQ54_05805"
     CDS             1445..2020
                     /locus_tag="PLQ54_05805"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS92806.1"
                     /translation="MTELADRALVARARRGDTEAFGALVDRHRQRVFGVAYRMLGDVE
                     AANDVAQEAFVRAFERLESFRSSGSFVAWLVSIASNLCIDIQRRSRRTPMSLDVMQEE
                     KGYDPADPRQAAIEHRLGTDEAADAIHQALEKLPAAQRLAITLRHIEGLGYEEIGQAM
                     GVPVGTAKTHVHRGRERLARMLHRFYAEVGS"
     gene            2020..2583
                     /locus_tag="PLQ54_05810"
     CDS             2020..2583
                     /locus_tag="PLQ54_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOS92807.1"
                     /translation="MECNRGRQMIHAELDGELTEERRALLAEHLGQCAACAQFRARLM
                     ALTTDLAALPLPAQPEGFRDAVMGQVEGLARKRRGRLWGLRPALPWVGVGLSSSLIYA
                     ALATGIVLGLPQWVSGAGERLAALLSAGHGLWQSSGAIWSYASSAAGDNAPLMVCGLA
                     AYLAVLAGAGWKFRRQLLPAMVGARDA"
     gene            2636..>3890
                     /locus_tag="PLQ54_05815"
     CDS             2636..>3890
                     /locus_tag="PLQ54_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOS92808.1"
                     /translation="MNSRWINRGVVWMAASACAGLVLCVGLAYAADRQVVGSDVTVGA
                     GETQRGDLVVLNGNAVVRGTVKGSVIALAGNVVLDREAGVRGDVVALGGTVENRGATI
                     SGKVAALPGKGLRELLAHVPSDGTKLGSLAEACAAEAGEGRVVVGKPVTIGADERVNG
                     DVIVLGSDLTVNGKVRGSAVAIGGALRVGDRADVADQALAVGGEARFDSGATLRGTAL
                     AVDNPWVKETVGKAVAAAGLAEASGAPAVRAEEEPRRAATPPPAGAIGGDQTWHESHT
                     IEDGQVYAGNVTVTRGDLTIRGRVDGNVTVVKGDIKMEGDAHVAGNVTTTVGDISLRD
                     RASISGNCVTVKGDIELHNEARIDGDAVATLGKVLPHDSATVKGNSVGVGGLGIAAHT
                     KPPSPPVPPVARAVARSPHSDLCGSV"
BASE COUNT          655 a         1208 c         1407 g          620 t
ORIGIN      
        1 ccgtgaactg ataggaataa gccgtcgacg ccatccccaa catacacacg gcgaacacgc
       61 ttgccaccag agctttcttc attgcacacg ctccctcgcc agtcaccccg atagaacctc
      121 aacaggagag ctccggggcc tacaggttcc cgatgggata ccctgcgcca atccatcaag
      181 aagtataacc agacgtcaca gacgtgtcaa catgttcgcc agaagaagtt tcagcccgcg
      241 cccgcggaac tacagggcac cgtagccatc gaggatccat agcccctcga agtcatcggc
      301 cgagctcttg ttgaggccga cggcgttggc cagcatgttg gccgatagtt ggcagaaggc
      361 cagtccgcgc gtggatcctc ccggcaggcc gtagctaaag gccacgtagc cgctggacag
      421 cacctggcac cccgtcaact cggagtcaaa aagaggtgac atgaggccga atgagccgta
      481 gaccgccgag gcgcgcgcgt tctcctggac gttggccgtg tagatgtagc tgagctcctt
      541 gctggcgtca taggtggcga gcaacagctt cccgtctggc atcccgcaca gtgcccggac
      601 ggcaccgcgg ggggtgagca cccggatggc gccggtgccg tcgggcgcaa tggcccagac
      661 gtcgccgtcg ggtccgacat aggcgatccg gccatcctcg agcgcggcag gaaggttggc
      721 agtctcggcg atgagggtcg cgtcgccgcc cgtggcattg acgacgaaga ggcggccatc
      781 agacacatag gcgatccggt cactggcggc acccttgagg ccgacagcgc gggctttcga
      841 cggggcaggg ttgatcgcca ggttcgtggc ctggccgccc gcgatgaccg cgagtcccgg
      901 agttccctcg gtgcgccagt agacgacgcg gccgtcggcc aggaagctcg gactgtgccc
      961 ggccacatcg gtgaggcgct ccactcgggt gccgttggag ccgatcgcga acaccccctc
     1021 acggcgttcg aggtggttgg cggcgttggt gccctcgtag accacaaaca ggatctcgtt
     1081 gccgagggga atcgtggcgg taatatccgt cgggaggacc agattgcgct cgttggattt
     1141 gtcgtctgaa ccgccggtga cctcggtgat ccattcacag ccgctaaggg ccagtagggc
     1201 ggtcagaaag agagtgcctc cgaggaccac tccgggcccg cgcttaggcg tcaacgatat
     1261 cgagctcctt ccccaggccg aggtacgggc cggtgccgga cgattggact cgcggcacac
     1321 ctcgcaggtt ccccgcaggc caacgccgct acggtatggt acgacaggct gctgcccgaa
     1381 gaaagatgaa acgctccgcc acccaggccc gtagagactc caagcagtgc acgggaggcc
     1441 gccagtgaca gagctcgcag accgcgcact cgtggcgcga gcgcgacgag gcgacacgga
     1501 ggccttcggt gcgctggtag accggcaccg ccagagggtc ttcggggtgg cttaccgtat
     1561 gcttggggac gttgaggcag ccaatgacgt ggctcaggag gcattcgttc gggccttcga
     1621 gcggctcgag agcttccgct cctctgggag cttcgtggcc tggcttgtct ccatcgcctc
     1681 gaacctctgc atcgacatcc agcggcggtc gagacgcacg ccaatgagcc tggatgtgat
     1741 gcaggaggag aagggctacg accccgccga tccgcgccag gcggccatcg agcatcggct
     1801 ggggacagac gaagctgctg atgccatcca ccaggccctg gagaagttgc cagcggcgca
     1861 gcggctggcg atcactctcc ggcatatcga gggcctgggc tacgaagaga tcgggcaggc
     1921 catgggagtg cccgtcggca cggccaagac ccatgtccac cggggtcgag agcggttggc
     1981 gcggatgctg caccggttct acgcggaggt ggggtcgtga tggagtgcaa tcgcgggcgt
     2041 cagatgatcc atgcggagct ggacggcgag ctgactgagg agcgtcgcgc gctcctcgcg
     2101 gagcacctgg gccaatgcgc ggcctgtgcc cagttccgcg cccgcctcat ggccttgacg
     2161 accgacctgg ctgcgctgcc gctgccggcc cagcccgagg gcttccgcga cgctgtcatg
     2221 ggccaagtcg aggggttggc gcggaagcgc cggggccgcc tctggggctt gcgccccgcc
     2281 ctgccctggg tgggagtagg actgagctcg agtctgatct acgcggctct cgcgaccggg
     2341 atcgttctcg gcctaccgca gtgggtgtcc ggagccggcg agcgcctggc ggccctactc
     2401 agtgcgggtc acggcttgtg gcagtcgagc ggcgcgatct ggtcctatgc ctcgagtgcg
     2461 gccggtgata acgcaccact catggtctgc ggcttggcgg cctatctggc cgttctggcc
     2521 ggcgcgggat ggaagttccg gcggcagctg ctgcccgcca tggtcggtgc ccgcgatgcg
     2581 tagcgccgcc atgcgagaga cgtctggtgc ccctaccgtt ggggaggcga gattggtgaa
     2641 cagcaggtgg atcaatcgcg gtgtggtgtg gatggcggca tcggcgtgcg cagggcttgt
     2701 gctctgcgtg ggtctggcgt atgccgccga ccgccaggtc gtgggctcgg acgtcacggt
     2761 gggcgccggc gagacccagc gcggtgatct ggtggtgctg aacggcaacg ccgttgtcag
     2821 aggaaccgtc aagggttcgg tgattgctct ggccgggaac gtggttctcg acagggaggc
     2881 cggagtccgg ggtgacgtgg tggccctcgg tggcacggtc gagaatcgcg gggccacgat
     2941 cagcggcaag gttgcggcct tgccgggcaa ggggctgcgg gagttgctcg cgcacgttcc
     3001 gtccgatggt accaagctgg gttcactggc cgaagcatgt gccgccgagg cgggcgaggg
     3061 gcgggtggtc gtgggcaagc cggtgaccat cggtgccgac gagcgagtca acggcgatgt
     3121 catcgtgctc gggtcggacc tgaccgtgaa cgggaaggtc cggggatcgg ccgtggccat
     3181 cggtggtgcc ctgagggtgg gcgaccgcgc cgatgtggcc gaccaagccc ttgccgtcgg
     3241 cggggaggca aggttcgact ccggtgctac ccttcggggg actgcactgg ccgtcgacaa
     3301 cccgtgggtg aaggagacgg tcggcaaggc cgtggcagcc gcgggtctag ccgaggcgtc
     3361 gggtgcgccg gccgtccggg cggaggagga gcccaggagg gcggcgaccc cgccaccggc
     3421 cggggccatc ggtggggacc agacctggca cgagagccac acgatcgagg acggccaggt
     3481 ctacgccggc aacgtgacgg tgactcgagg cgatctcacc atccggggcc gcgttgacgg
     3541 caacgtgacc gtggtcaagg gcgacatcaa gatggaaggg gatgcccatg tcgccggtaa
     3601 cgtcacgacg accgtcggcg acatatcgct ccgcgaccgg gcctcgatca gcggcaactg
     3661 cgtgactgtc aagggcgaca tcgagctgca caacgaagca cggatcgatg gcgacgccgt
     3721 ggcgaccctc gggaaggtgc tgccccacga cagtgcaact gtcaagggaa actcagtggg
     3781 cgtgggtggc ctgggcattg cggcgcacac caagccgccc tctccgccgg tcccgcccgt
     3841 ggctcgggcg gtcgcccgca gtccccatag tgacctgtgt ggatcggtgg
//
LOCUS       DAOSPL010000241         3887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_23482_length_3887_cov_3.578810, whole genome shotgun sequence.
ACCESSION   DAOSPL010000241 DAOSPL010000000
VERSION     DAOSPL010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23482_length_3887_cov_3.578810"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3582)
                     /locus_tag="PLQ54_05820"
     CDS             complement(<1..3582)
                     /locus_tag="PLQ54_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS92809.1"
                     /translation="MRIHTRLWLAAALLMLFFIAALVIVQARIASTMALMSRVREREM
                     DQTLGRVVELSGSPPATFTADRCRSVEVASLVRSRSAQRVAELLRPSLSLYGVDGVWV
                     YDASCVPIAVCVGPSSAVPGDPPFGRDALRELCEGAQAGHFYMALESTVLELWYGAIQ
                     PSAAGGDRDEQTPVGWLLAGRILSDDVLQSVADVLGGEVYLLRARPPRGTPPAPLDEP
                     AGAGPSQLGPGPKGRTSLAESVRELAVDEAPPPPDKAGSPPPPPMESLSPGRTAPGPP
                     TLTSIMALCRLHDHKGRPVAVLEARVPSPATRLLRDGARASILLGSGLALGLLGAVTY
                     ILIAWMANPLRRVSRALETAQRDPIAPLMGQGHEFGKVADLIDAFFRQRHELIEALEQ
                     QRAVSEALRATEERHELAMAATSASVWEWDLVTGRMHVGARLYTMLGLEPGELDVPFG
                     AFHSRMHPDDLPSVQATIRDHLGPGAGGFTTEYRLATAWGGWVWIMSRGDVVARDDDG
                     RPIRFVGTHVDITERREMELALRESEERLRTIAAAAQDAVIMMDHEGRITYWNPAAER
                     IFGWLEHEALGRELHTLVAPPETHEAYRAGLRQFQASGTGPALGRLLQLEARVREGRT
                     IPVEVSISSVRMGGQWCAVGIVRDITERVEAQRRLESALRTAQEMAVAAEAASAAKSE
                     FLANMSHEIRTPMNAVIGMTELVLDAELTAEQREYLETVRSSGEALLDIINDVLDFSK
                     LEAGRLSIEHTNFGLRSAVEAAVSSLAVRAHEKGLELVCRIAHDVPELLIGDPGRLRQ
                     VVVNLIGNAIKFTPQGEVEFRVEVASEDDDEVELCFSVSDTGIGIAADKLERIFESFT
                     QADGSTTRQYGGTGLGLAICRRIVELMGGHIWAESEEGKGSVFRFRARFGVRERAAVT
                     PADASLEGARVLVVDDSATNRAMLRECLTAWGVEVLELSTGDEAVAFLEGEVASGRGC
                     QAVLMDRAMPGADGFEVARRIAAHPLLSHVPVMMLTSAGRRGDVARCREMGVAGYLMK
                     PVRQSELRQALRMALARGTCLEAATEPITRHTVRQARRQLRVLLAEDHPVNQKLATRL
                     LERAGHVVTVVADGQEALDVFEPGRFDVILMDVQMPRMDGVQATMAIRLKEHQHPNAA
                     HVPIIALTAHALATDRTRFIAAGMDDHVAKPVTADTLLGTVERLTRPS"
BASE COUNT          623 a         1407 c         1251 g          606 t
ORIGIN      
        1 cgagggccgg gtcagccgct caacggtgcc gagcaacgtg tcggccgtga ccggcttcgc
       61 cacatggtcg tccatcccgg cggcaatgaa ccgcgtccgg tccgtcgcca aggcgtgggc
      121 ggtcagggcg ataatgggaa catgcgccgc attcgggtgt tggtgctcct ttagccggat
      181 cgccatggtg gcctggactc cgtccatccg cggcatctgg acgtccatta ggatcacgtc
      241 gaagcgccca ggctcgaaca catcgagggc ctcctggcca tccgccacga ctgtcacgac
      301 gtggccggcc cgctccagca gtcgggtagc cagcttctgg ttgaccgggt ggtcctcggc
      361 caggagcact cgcagctgcc tccgggcctg ccgaacggtg tgccgcgtga tgggctcggt
      421 cgccgcctcg aggcatgtgc ctctcgccag ggccatccgc agcgcctgcc gcagctccga
      481 ctggcgaacc ggcttcatca ggtacccggc cacacccatc tcgcggcacc gggcgacgtc
      541 gccgcggcgt ccggccgatg tcagcatcat gactgggaca tgggaaagca gcggatgcgc
      601 cgcgatgcgg cgcgcgacct cgaagccatc ggcgcccggc atcgcgcggt ccatgagcac
      661 ggcctggcac cccctccccg acgccacctc cccttcgagg aacgccacag cctcgtcgcc
      721 cgtggacagc tcaagtacct cgactcccca ggccgtcagg cactcacgca gcatggcgcg
      781 attcgtggcg ctgtcgtcca ccaccagcac ccgagcgccc tcgagcgatg catcggccgg
      841 ggtgactgcc gcccgttccc gaaccccgaa ccgcgcccgg aaccggaaga cgctgccctt
      901 gccctcctcg ctctcggccc agatgtgccc tcccatgagc tcgacgatgc gccggcagat
      961 ggccaggccc aaccccgtgc caccatactg gcgggtggtc gagccatcgg cctgtgtgaa
     1021 ggactcgaag atcctctcga gcttgtcggc agcgatgccg atgccggtgt cggagaccga
     1081 gaaacacagc tcgacctcgt cgtcgtcctc gctcgcgacc tccactcgga actccacctc
     1141 gccctgcggt gtgaacttga tggcgttgcc gatcaggttc accacgacct gacggagcct
     1201 tccggggtct ccgatcagta gctccggcac atcgtgggcg atgcggcaca ccagctcgag
     1261 gcccttctcg tgcgcacgta ccgccaggct gcttacggcc gcctccaccg ccgaccgcag
     1321 tccgaagttc gtgtgctcga tcgaaagccg tcctgcctcg agcttcgaga agtccagcac
     1381 atcattgatg atgtcgagca gggcttcgcc agacgatcgc acggtctcga ggtactccct
     1441 ctgctcagcc gtcagctcgg cgtcgagaac cagttccgtc atgccgatga cggcgttcat
     1501 cggggtccgg atctcgtggg acatgttggc caggaactcg ctcttggcgg cgctggcggc
     1561 ctctgcggcc accgccatct cctgcgccgt acgcagcgca ctctccagcc ggcgctgtgc
     1621 ctcgacccgt tcggtgatgt cccgcacgat cccaacggcg caccactggc cacccattcg
     1681 tacgctcgag atcgacactt cgaccggtat ggtgcgacct tcccgcaccc tggcctcgag
     1741 ctgaagaaga cgtcccagtg ccggaccggt gcccgaggcc tggaactgcc tcaggccggc
     1801 ccggtacgcc tcgtgggttt ccggcggcgc caccagcgtg tggagctctc gccccaaggc
     1861 ctcgtgctcc agccagccga agatccgctc cgccgccgga ttccagtacg tgatccgacc
     1921 ctcgtggtcc atcatgatca cggcgtcctg ggcggcggcg gcgatagtac ggagccgctc
     1981 ctcgctctct cgcagagcga gctccatctc gcggcgctcg gtgatgtcca catgcgtgcc
     2041 gacgaaccgg atcggacgtc cgtcgtcgtc gcgcgccacc acatcgcctc ggctcatgat
     2101 ccacacccag ccgccccagg ccgtggcgag gcggtactcg gtggtgaacc ctcccgcacc
     2161 ggggcccaga tggtcgcgaa tcgtggcctg aaccgacgga aggtcgtcag ggtgcatccg
     2221 ggagtggaag gcgccgaagg gcacatccag ttcgccaggt tcgaggccca gcatggtgta
     2281 cagccgggca ccgacatgca tacgcccggt gaccaggtcc cactcccaga cgctcgcact
     2341 cgtggcggcc atggccagct cgtggcgctc ctcggtcgcg cggagcgcct ccgacaccgc
     2401 tcgctgctgt tcgagcgcct cgatcagctc gtgccgctgg cggaagaacg cgtcgatcag
     2461 gtccgcgacc ttgccgaact cgtgtccttg ccccatcagc ggcgcgatgg ggtcgcgctg
     2521 ggccgtctcc agggcccgcg agacccgtcg cagagggttg gccatccacg cgatcagtat
     2581 gtacgtgacg gctccgagca gtcccagagc taacccactg cccaggagta tgctggcccg
     2641 cgcgccgtcc cgcagtaggc gcgtcgccgg tgagggcacc cgggcttcga gcacggccac
     2701 aggtcgtccc ttgtggtcat ggagacggca cagggccatg atggaggtaa gcgtgggggg
     2761 cccaggcgct gtccggccgg ggctcaggct ctccatgggg ggaggcggag gcgagcccgc
     2821 cttgtcggga ggcgggggcg cctcatccac cgcgagttcg cgcacggact ccgccagact
     2881 ggtgcgtccc ttcggtcccg ggccgagctg tgaaggtcct gcccccgccg gctcgtccaa
     2941 gggagccggc ggcgtccccc gcggcggacg agcccgcaag aggtacacct cgccgcccag
     3001 gacatcggct acgctctgga gcacgtcgtc cgacaggatt cggccggcca ggagccatcc
     3061 cacgggggtc tgctcatccc tgtcgccgcc agcggctgat ggctggatgg cgccatacca
     3121 gagctcgagc accgttgact cgagcgccat gtagaagtgg cctgcctgag cgccttcaca
     3181 cagctcacgg agcgcgtccc gcccgaacgg cggatctccc ggcaccgcgg aactcggacc
     3241 cacgcacacc gcgatcggca cacacgacgc atcgtacacc cacacgccgt ccacgccata
     3301 caggctcagc gaaggccgga gcagctcggc cacccgctga gccgacctgc tgcgaacgag
     3361 cgaagcaacc tcaaccgacc ggcatcggtc ggcggtgaac gtcgcgggag ggctacccga
     3421 tagctccacg acgcgtccca gggtctggtc catctcgcgc tcccggaccc tggacatcag
     3481 ggccatcgta ctcgcaatac gtgcctgcac gatgaccagc gcggcgatga agaacagcat
     3541 cagaagcgcg gcggcgagcc agagcctggt gtgaatccgc atcagtcggt cggctccccc
     3601 ctcgcgaaca tgcgccgcca ccgctgcccc gtgttcagga gcggggcgac agtaaaagtg
     3661 tgccgtatgg gcattgtggt cggctaccgc atggcgcgcc gagcgggtgc cgcgaatgcc
     3721 cccgaaccat gtttgctcaa ctccttgccc atccttggcc acgcgttgcc ggcgcggata
     3781 cctcccccct gcgagtcact gagccgcgcg cggcgggcgc tcgcacgact gctgagagga
     3841 atcaacggag aggagaggtg gggggcatgg cgaagaggaa tgtgagc
//
LOCUS       DAOSPL010000242         3882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_23520_length_3882_cov_2.700810, whole genome shotgun sequence.
ACCESSION   DAOSPL010000242 DAOSPL010000000
VERSION     DAOSPL010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23520_length_3882_cov_2.700810"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..1714
                     /locus_tag="PLQ54_05825"
     CDS             269..1714
                     /locus_tag="PLQ54_05825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS92810.1"
                     /translation="MAKQAVLACGVVDDLWGQVLIDHLEGRSAQYRLVFDSGYEVVFD
                     AAEHFSPYFQPHELEALSFAHGRILDIGPGAGRHMLWALSKGFRPTGVDRSPLAVEAC
                     RRRGCEDVTCADILNVELPPAHFDSVLLMGNNMGLAGSVDGTAHLLRIAFEAAAPGAW
                     LIGSFVAMDHVPDGERPERGVVARHRIAYRSQVGPLFDWTFFSTEQLVSLAREQGWQI
                     RRVLSGTDIQSYLIAAKPLQGIEALLGLPERELDCDLWEGVLSRAWLGEVADLEIEGF
                     GSPPRDLVDVDAVLGPGVSDAETAALSRLRGRVLELGCGAGAALSFLTDRGCSVIGLD
                     SSDLAVGAAHMRGISRARRADWLDSLPDDGPFAGFVLLHSRAALAGDLPGVRLLLERL
                     HSTGTPDAVLVMSYEISGVSFPGLARYVHQGRAGPWWYFAQFETDEAVSDLMERAGWR
                     CVELLDTGPTGRIAIARRAVHAGLPVPGMGE"
     gene            1720..>2364
                     /locus_tag="PLQ54_05830"
     CDS             1720..>2364
                     /locus_tag="PLQ54_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS92811.1"
                     /translation="MRVAYMVNTPDVDPRGVLGMTGDVAENLGLLADLGFGAVELQVR
                     DPSRIDRPAIERALDAVGLAVCCVCTGEVLPQDGLSLTDPNPVVRDLALARLRDMIDL
                     ASTWGCCVNIGRLRGQYVAGVPERETRTWGLDAFTRAADYAEPRGVTLVLEPIAPPFC
                     NWINTTHEGISMYLKVARASLRLMLDTAHLEAVGEEPGHAVETAAHRLAHVHIAG"
     assembly_gap    2365..2374
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2556..>3882)
                     /locus_tag="PLQ54_05835"
     CDS             complement(2556..>3882)
                     /locus_tag="PLQ54_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92812.1"
                     /translation="VRLATRRGELPTCVAALADGWLTLDQAAEIARHVPAAFDADGNT
                     LGVAEIRWANGKCWLMPVEKGVRYEVKPDSGAPRAALEIAGSPTEVAPGQEVTIDVQS
                     AAPAQRDELSVSARGRTLEASVRGVEPGRSRVTFVVPADAEPGRALWVRVAPADADQA
                     AWLVLNVRRLAELSVSPATVVLRGGEATVNLSGRVGVSGQPATLAIEAPEGLSVEPRH
                     IEGGPEIHAQLELRVVGAVAGELRLVAQAGPLCEVHAFELLPGEARPNELDFADGSIP
                     FSWGQCFRGGEERRGDVQTGSNCYASTGLSSGGVERRGLFTHPPYNGGVGYTYIDFEP
                     TVVPAEPCEMHLFIGLLDGGDPSDGVDFIVEARLDDGTRVPLMKENTAARAWREVVAD
                     LEPVRGKRVSFRFIADVGPNDNSASDWGCWGEPAVRLKAAVPTVTLVPR"
BASE COUNT          562 a         1302 c         1375 g          633 t
ORIGIN      
        1 cgtagttggg acagctcgca gaacggcgcg gcgttcccgg ctcggccctc cgccggcacg
       61 agggcggcgg cgcaggcagc caggagcggg gcggtgcatc gcatggcggg tcctcctgag
      121 cggtggcgag tcatggttcg cggcgatgcc cgtccgacct cccggtgtgg ggtggcgtcg
      181 tgggtgtgga acaagggcat tgggggccgt ccgcgaatct gactggtggc tgccgcacgc
      241 ggcaaggcga tcccggaggc ggaggcgcat ggccaagcag gctgtgttag cctgcggcgt
      301 agtggatgat ctgtggggtc aggtactcat cgaccacctg gagggacggt cggcgcagta
      361 ccggctcgtg ttcgacagcg ggtacgaggt cgtgttcgat gcggccgagc acttcagccc
      421 gtactttcag ccccacgaac tggaagcgct gtcgttcgcc catgggcgca tcctcgatat
      481 cgggcccggt gcgggccgcc acatgctgtg ggcgctctcg aaaggctttc ggccgacggg
      541 cgtggaccga tcacctctgg ccgttgaggc ctgccgccgc cgtgggtgtg aggacgtcac
      601 ttgtgcggac atcctcaatg tggagctgcc ccctgcccac ttcgattctg tgctcctgat
      661 gggcaacaac atgggcctgg cgggttctgt cgatgggaca gcgcatctgc tgagaatcgc
      721 gttcgaggcc gcagcaccgg gcgcgtggct catcggctcc ttcgtagcca tggaccatgt
      781 ccccgatggc gagaggcccg agcgcggtgt cgtggcccgg catcgcatcg cctaccgcag
      841 ccaggttggg cccctcttcg actggacctt cttctcgacc gaacagcttg tgtcgctggc
      901 tcgcgagcag ggctggcaga tccgccgggt gctatcgggc accgatatcc agtcgtatct
      961 gatcgccgcc aaaccgctcc agggcattga ggcgctattg ggtctgcccg agcgggagct
     1021 ggactgtgat ctgtgggaag gcgtcctgag ccgagcctgg ttgggggagg ttgcagacct
     1081 ggagatcgag gggttcggct cgccgccgcg ggacctggtg gacgtcgacg ccgttctcgg
     1141 cccaggcgtg agcgacgccg agacggctgc tctgtctcgc ctgcgaggcc gtgtgctgga
     1201 gttgggatgc ggggccggtg cggcgctctc gttcctcacc gaccgggggt gctcggtcat
     1261 cgggctcgac tccagcgacc tggccgtggg ggccgcgcac atgcgcggga ttagccgcgc
     1321 ccgccgcgcg gactggttgg actcgctgcc cgacgacggg ccattcgctg gcttcgttct
     1381 actccacagc cgcgcagcgc tggcaggcga tctgccgggt gtccgtctgt tgctggagcg
     1441 gctgcactcg acgggaacgc ccgacgcggt gctggtgatg tcgtatgaga tctctggcgt
     1501 gtctttcccg ggtctggcgc gctacgtgca tcagggtcgg gcgggcccct ggtggtactt
     1561 cgcccagttc gagacggacg aggcggtgtc cgatctgatg gagcgcgcgg gttggcggtg
     1621 tgttgagctt ctggacaccg ggccaaccgg ccgaatcgcc atcgcccggc gcgccgtgca
     1681 cgcaggcctc ccagtgcccg gcatggggga ataggcccca tgcgggtcgc ttacatggtc
     1741 aacacaccgg acgtggaccc tcgcggcgtg ctcgggatga cgggcgatgt ggccgagaac
     1801 ctgggcctct tggccgatct tggcttcggg gccgtcgagc tgcaggtacg cgacccctcg
     1861 cgcatcgacc ggccagcgat cgagcgggcc ctggacgcag taggcctggc cgtgtgctgc
     1921 gtgtgcacgg gtgaggtgct gccgcaggac gggctgagcc tcacggaccc gaaccctgtg
     1981 gtgcgggatc tggccctcgc ccgattacgg gacatgatcg acctggcgtc cacatggggg
     2041 tgttgtgtga acatagggcg cctgcgtggc cagtatgtgg ctggagtacc cgagcgcgag
     2101 acgcggacgt ggggcctcga cgcgttcacg cgggcggctg actatgccga gccccgaggc
     2161 gtgaccctgg tgctcgagcc gatcgctcct ccattctgta actggatcaa caccacgcac
     2221 gagggcatca gcatgtacct gaaggtagcg cgcgctagtc tccggctgat gctcgatacg
     2281 gcccacctcg aggccgtggg agaggagccg ggccacgcgg tcgagacggc cgcacatcgt
     2341 ttggcccatg tgcacatcgc cggcnnnnnn nnnngtcagc gacgcgggcg attggctaca
     2401 ccggggcgct gtcagccgag ctccgggccc aggaccaccg cgaggcggcg cggcgcacga
     2461 tggaggccct gctgccactg ctgggctgac aggcgcccaa acggtcggaa acgctcggcg
     2521 gccttctggc ggacccgggg ggccagtccg ccacactatc tgggcacgag cgtcaccgtg
     2581 ggcaccgcgg ccttcagccg caccgcgggc tcgccccagc atccccagtc cgaggccgag
     2641 ttgtcattgg gcccgacgtc ggcgatgaag cggaagctca cacgcttacc gcgaaccggt
     2701 tccaagtcgg ccacgacttc acgccaggct cgggccgcag tgttctcctt catcaagggc
     2761 acccgcgtgc cgtcatcgag ccgggcttcg acaatgaaat caacaccgtc ggaggggtcg
     2821 cccccgtcca ggaggccgat aaacaggtgc atctcgcacg gctcggcggg caccacagtg
     2881 ggctcgaagt cgatgtacgt atagccgacg ccgccgttgt agggcgggtg ggtgaagagc
     2941 cccctgcgct cgactccacc cgaggagaga cccgtactgg cgtagcagtt cgagcccgtc
     3001 tgcacatcac cccgtcgctc ctcccccccg cggaagcact ggccccacga aaaggggata
     3061 gagccgtcgg cgaagtcgag ctcgttgggc cgcgcctcgc ccggcagcag ctcgaatgcg
     3121 tgcacctcgc agagcgggcc ggcctgggcc accaatctga gttccccggc cacggccccg
     3181 accacacgga gctcgagctg tgcgtggatc tcgggccctc cctcgatgtg ccgaggctcg
     3241 accgacagtc cctcgggcgc ctcgatggcg agcgtggcgg gctggcccga cacgcccacg
     3301 cggccactga ggttgaccgt cgcctcgccg ccgcggagca ccacggtggc cgggctcacg
     3361 ctcagctcgg cgagccgccg gacgttcagc accagccatg ccgcctggtc ggcgtccgcg
     3421 ggcgccaccc gcacccacaa cgctcggccg ggctccgcgt ccgccggcac aacgaaggtg
     3481 acacgacttc gaccgggctc gacgccccgt accgaagcct cgagtgtgcg gccccgggcg
     3541 gacacactga gctcatcgcg ttgcgccggc gcggcactct gaacgtcgat ggtcacctcc
     3601 tgacctggtg ccacctccgt cggtgaacct gcgatctcaa gggcggctct gggggctccc
     3661 gagtccggct tcacctcgta ccgcacaccc ttctccaccg gcatgagcca gcacttgccg
     3721 ttggcccagc gaatctcggc gacgcccaga gtgttgccgt cggcatcgaa ggcggccggc
     3781 acgtgccggg cgatctccgc ggcctggtcc aaggtgagcc agccgtcggc cagggcggcc
     3841 acacacgtgg ggagctcgcc tcggcgcgtg gccaagcgga cc
//
LOCUS       DAOSPL010000243         3879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_23546_length_3879_cov_3.521444, whole genome shotgun sequence.
ACCESSION   DAOSPL010000243 DAOSPL010000000
VERSION     DAOSPL010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23546_length_3879_cov_3.521444"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="PLQ54_05840"
     CDS             <1..980
                     /locus_tag="PLQ54_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92813.1"
                     /translation="MDDITGIYGGHHYINDRPLQDLRFYPWFESKVKWGVDLARSKGK
                     DFILGEFGCKQDGRTLDGKRADRCVYFETPEEPLVGIQLAEAVIAAINAGVYAMGNWT
                     YMDFPDDYRPDYLNKWGTFRWSGDDFSTRAHYYAYGLLTKFLRGPSTILRTEVDDPHV
                     RVAAIEHHGAGTRTIAIVNRYGGDVPVSIAMGDAANGHTFRKYVYDPEHVPQNRFGDL
                     QRPETTVTVRRGTLDDQIGRGTLTVYTTAYDDEPPAAVTGLRIETHTDGSTWAAWDEN
                     PAPDLCYYRIFRGDEQIGSTVACRFRVAAGDTADGLRIVAVDQSGNPGV"
     gene            complement(995..1720)
                     /gene="rph"
                     /locus_tag="PLQ54_05845"
     CDS             complement(995..1720)
                     /gene="rph"
                     /locus_tag="PLQ54_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010270989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOS92814.1"
                     /translation="MRTDGRQPAEMRPVTLVTPCTKHAEGSCRIEVGDTVVLCTASVT
                     DRVPPFLRDSGRGWVTAEYGMLPRSTSTRTDREAAKGKQSGRTLEIQRLIGRALRSVV
                     DLTQIGDRTITIDCDVLQADGGTRTASITGGFVALAQALQWLKTERNAFPRIPLNDPV
                     AAISVGVVQNQLLLDLNYAEDSDAMVDMNVVMTGRGCFVEVQGTAEAFPFTRQRLDDM
                     LDLSAKAIQDLIAKQREALGGLV"
     gene            complement(1692..2102)
                     /locus_tag="PLQ54_05850"
     CDS             complement(1692..2102)
                     /locus_tag="PLQ54_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92815.1"
                     /translation="MGAVERAQEVRPADWEGDWESKEFPGLTLTVTGASVEGVYAGGQ
                     GHIQGEIRGSGIDFQYWRGATEFLGAALDQRGLGSGALSTSGLSVKGTYTSDTAVEGK
                     WSMKRPGQPDEAPEAAPSKGETPSDGENRRPPAR"
     gene            complement(2374..2859)
                     /locus_tag="PLQ54_05855"
     CDS             complement(2374..2859)
                     /locus_tag="PLQ54_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92816.1"
                     /translation="MTMVEGWPKHSTSELLAVPPGMRVTQCIGLGYQSMAESIAVVLE
                     PGKGVGLGEIWRIDDLAAVVQKGARPSAKLLARFYCDPGRTRLVLPAKRGQGLAAVWV
                     LPDSPRASVRAADLSGPSARWLPVTTLPPEDGWDLRAYERARRWIERNAADSEVPVLP
                     L"
     gene            complement(2856..>3879)
                     /locus_tag="PLQ54_05860"
     CDS             complement(2856..>3879)
                     /locus_tag="PLQ54_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92817.1"
                     /translation="VLAGSPGGARSSVVESAPARTRRGPSPVAYVAVPLALIALAVIL
                     RSRRGLGPIAALAAVASLSLPGHVRAGPGGGSTPEWVMLLGQAQEAIEDAVKAFAADT
                     GCYPASLADLTLGGQVSRGVDASGNPVRLMVRPTRAYLAELPVDVLTERSDTWLMSVA
                     GGAVVQSGGLEGVVRREHTYGRQDVKSWGWSNPGFAVGRAHVGSRRDRSLPERTDSAP
                     ETRSFWASEVVREGRFAAIASPAGDKPWGVVAVGKDETAVLLGSGMTTPTTGPDAPKP
                     GYARAVEGWGSGRRSEGLTSWCGSRPRRDHRGRPNWNSATCRPKYWFSVSDAGPSSSV
                     WGTVRR"
BASE COUNT          623 a         1475 c         1233 g          548 t
ORIGIN      
        1 acatggatga catcaccggc atctacggcg gccaccacta catcaacgac cggccgttgc
       61 aggatctccg cttctacccc tggttcgagt cgaaggtgaa gtggggcgtc gatctcgctc
      121 gcagcaaggg gaaggacttc atcctcggcg agttcggctg caagcaagac ggccgcacgc
      181 tcgacggcaa gcgcgccgat cgctgcgtgt acttcgaaac ccccgaggag ccgttggtcg
      241 gtatccagct cgccgaagcc gtgatcgcgg ccatcaacgc gggcgtctac gccatgggca
      301 actggaccta catggacttc cccgacgact acagacccga ctacctcaac aagtggggca
      361 ccttccgctg gagcggcgac gacttctcca cccgcgccca ctactacgcc tacggcctgc
      421 tgaccaagtt cctccgaggc ccctcgacga tactgcgcac cgaggtagat gatccccatg
      481 tccgcgtcgc cgcaatcgaa caccacggcg ccggcacgcg taccatcgcc atcgtgaacc
      541 gctatggggg cgatgtgccc gtctccatcg ccatgggcga cgccgcgaac ggccacacgt
      601 tccgcaaata cgtctacgac cccgaacacg tgccgcagaa ccgcttcggc gacctgcagc
      661 gccccgaaac gaccgtcacc gtgcgccgtg gcacgctcga cgaccagatc gggcgcggca
      721 cgctcacggt ctacacgacc gcctacgacg acgagccgcc cgcggcagtg accggcctgc
      781 gcatcgagac ccacaccgac ggcagcacgt gggccgcatg ggatgagaac ccggcccccg
      841 acctgtgcta ctaccgcata ttccgcggag acgaacagat cggctccacc gtggcgtgcc
      901 gcttccgcgt cgcggccggt gacacggccg atgggctccg aatcgttgcc gtggaccagt
      961 cgggcaatcc cggggtgtag gaggccgctc cgtcctacac cagcccgccc aacgcctcgc
     1021 gctgtttggc gatcaggtcc tggatcgcct tggccgagag gtcgagcatg tcatcgagcc
     1081 gctggcgggt gaagggaaac gcctccgcgg tgccctgcac ctcgacgaag catcctcggc
     1141 ccgtcatgac cacgttcatg tcaaccatgg cgtcgctgtc ctcggcgtag ttgaggtcga
     1201 gcagcaactg gttctggacc acaccgacac tgatggcggc aaccgggtca ttgaggggga
     1261 tcctcggaaa ggcgttgcgt tcggtcttga gccactgcag cgcctgggcc agagccacga
     1321 acccgccggt gatcgaagcg gtgcgcgtgc cgccgtccgc ctggagcacg tcgcagtcga
     1381 tggtgatggt gcggtccccg atctgcgtca ggtcgaccac cgaccgcagc gcccggccga
     1441 tgagccgctg aatctccaga gtgcggcccg actgcttccc cttggcggcc tcccgatccg
     1501 ttcgggtcga ggtcgagcgc gggagcatgc catactcggc cgtgacccag ccgcgtccgg
     1561 aatcacgcag gaagggagga acacggtcgg tcaccgacgc cgtgcacagc acgaccgtgt
     1621 caccgacctc gatgcggcac gaaccctccg catgcttcgt gcagggggtc accagagtca
     1681 ctggccgcat ctcagcgggc tggcggccgt cggttctcac catcgctagg ggtctctcct
     1741 ttggagggtg cagcttccgg cgcctcatcc ggctgcccgg gacgcttcat ggaccacttg
     1801 ccctcgacgg cggtgtcgga cgtgtacgtg cccttcaccg aaagaccgga ggtactgagc
     1861 gccccgctgc cgaggccgcg ctggtcgagc gcggccccca ggaactcggt cgcgccgcgc
     1921 cagtactgga agtcaatgcc actgccgcgg atctcgccct ggatgtggcc ctgtccgccg
     1981 gcgtagacgc cctcaaccga tgcgccagtc actgtcaggg tgagcccggg gaactccttg
     2041 ctctcccagt cgccctccca gtcggccggg cgaacctcct gggcgcgctc gacagctccc
     2101 accgcctcgg agctggtggc cgtagcacgg tcttgtgtcg ccccggcggc agccggccgc
     2161 tccggacctg aactgaggcg cttgcccgag cgcgagcaac cgccggcggc aagcaacccc
     2221 gccgccaggc agaaagcgag ccacgcgcgc cacgcatgag ccagagccac cgcccccaga
     2281 cgacccactg cggctgaggg tagcaggccc ctgtcgcggg ggtcaaggaa cgccccgtgc
     2341 ctcggctccg cgctccgagc ccctccccac tccctacagc ggcagcacgg gcacctcgct
     2401 gtccgccgcg ttgcgctcga tccagcgccg ggcgcgctca tacgccctca ggtcccagcc
     2461 atcctcgggc gggagggtcg tcaccggcag ccagcgagcc gaggggccgc tcagatcggc
     2521 ggcgcgtacc gacgcccgtg gcgagtcggg caggacccag accgcggcca gcccctggcc
     2581 tcgcttcgcc ggcaacacca gtcgcgtccg gcctggatcg cagtagaacc gtgccagcag
     2641 cttcgccgat ggcctggcgc ccttctgcac gacggccgcc aggtcgtcga tacgccagat
     2701 ctcgcccaga cccacgccct tgcccggctc gaggaccacg gcgatcgact cggccatgct
     2761 ctggtagccg aggccgatgc actgggtcac cctcatgccc ggcgggaccg ccagaagctc
     2821 ggacgtcgag tgcttcggcc agccctcgac catcgtcaac gccggactgt cccccagacg
     2881 gacgacgatg gcccggcgtc cgaaaccgag aaccagtact tcgggcgaca cgttgctgag
     2941 ttccagttcg ggcggcctct gtgatcccgc cgcgggcgcg agccgcacca gctcgtgagc
     3001 ccctccgacc gccttccgga tccccagccc tcgacggcgc gcgcgtagcc cggcttgggg
     3061 gcgtccggcc cggtggtcgg agtcgtcatg cccgaaccca gcaggaccgc ggtctcgtcc
     3121 ttcccgacgg ccaccacacc ccagggcttg tcgcccgcgg gagacgcgat cgccgcgaag
     3181 cggccctccc gcacgacctc gctggcccag aacgagcgcg tctcgggcgc cgaatccgtc
     3241 ctctccggca ggctcctgtc gcgtctggat cccacatgcg cccggcccac cgcaaagccc
     3301 gggttgctcc agccccacga cttcacgtcc tgccggccat aggtgtgctc gcgcctcacc
     3361 acaccctcga ggccacccga ctgcaccacg gcgccgccgg ccacagacat cagccacgtg
     3421 tccgaccgct cggtcagcac gtccaccggg agctcggcga gataggctcg tgtcggccgg
     3481 accatcagac gcacggggtt cccactggcg tcgacccctc ggctgacctg cccgcccagc
     3541 gtgaggtctg ccagggaggc tgggtagcac cccgtgtcgg cggcgaacgc cttcacggcg
     3601 tcctcgatgg cctcttgcgc ttgccccagc agcatgaccc actcgggcgt gctcccgccg
     3661 cccggaccgg cacgcacgtg accggggagg ctcagcgacg cgaccgccgc cagggcggcg
     3721 atggggccga gcccgcgccg gctgcggagg atcacggcca aggcgatgag cgcgaggggc
     3781 acggccacgt aggccaccgg tgacggcccc cgccgggtcc tcgccggggc cgattccaca
     3841 accgaggacc gcgccccgcc aggcgaaccc gccagcacc
//
LOCUS       DAOSPL010000244         3873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_23587_length_3873_cov_3.350707, whole genome shotgun sequence.
ACCESSION   DAOSPL010000244 DAOSPL010000000
VERSION     DAOSPL010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23587_length_3873_cov_3.350707"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..887
                     /locus_tag="PLQ54_05865"
     CDS             42..887
                     /locus_tag="PLQ54_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92818.1"
                     /translation="MRFQSRGILGLCLACVMTLPAYAQTVSLRYQPPAGMRTAYDMEM
                     SGEGKVQVATQAGAQETPLNLVAGMRASEKVLQAGTDGTRLVEMQVLAANATYNGQSV
                     PLNLAGRTMRFKQDVLGRVYGFEGQAPSSTAVGGAAGFDPLSLVNDLSRVVRFPEKPV
                     SVGQSWRLEYQVRTPSGGAPLVSCESILRQVGNANGRPTATVDHKLRVPLSIEQGGIR
                     LVGTLNANMRSTYYTDYGMLAASQWRAAIRLTAYGVGANGATTVLGSCTIPDLRATVR
                     QSGSS"
     gene            910..1656
                     /locus_tag="PLQ54_05870"
     CDS             910..1656
                     /locus_tag="PLQ54_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS92819.1"
                     /translation="MELHPEALLVTAPSVALALYYVPRMYLDRALTGSEAAAASGIVL
                     GLCGVCHSIWGAAPGYALGVVIIAAIGCLPYARRMLSRRDELAVAERELAEWRRTIAF
                     DARNRGAYVYAAEALRNLGRYAEAVSLYERAMALGPRDPAVQRALEVTTQLKRASAGE
                     NWICATCRAENAARIRECFRCGAHLPLAGAAGSPVAAYGIAPLAALVVPASALGICGA
                     ISPSAMLLLCWLGALGVVVFYAIVSPASED"
     gene            1666..2250
                     /gene="pth"
                     /locus_tag="PLQ54_05875"
     CDS             1666..2250
                     /gene="pth"
                     /locus_tag="PLQ54_05875"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOS92820.1"
                     /translation="MTDAFEPRIQLILGLGNPPREYAGTRHNVGADVVDRVAARMGIR
                     TWSHRWRSLSAEGHIGGCKVTLVKPMTYMNRSGLAAQAALQELRLLPSQLLVLADDVN
                     LELGRLRLRRKGSAGGHLGLESIVQHLGTTDFPRLRIGVGGPVTRAQLVEHVLSTLSD
                     EELEVLSPRLDEAADAVYDCVRVGIDHAMNAHNR"
     gene            2275..>3873
                     /locus_tag="PLQ54_05880"
     CDS             2275..>3873
                     /locus_tag="PLQ54_05880"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOS92821.1"
                     /translation="MVRRTLVWLAAGYGAGAVAGLATWRGLAAEGPSIAASAAVERLM
                     SWPWIVRSLVALLCVSTIGLPRLPRPGVRAVLALAAGLLVGTTAMLRHAVTRWTLPAY
                     VPGQKVTLTGTALESAAPGWGLRRVPLRVAEFRTSPGARPTDRTVLAHMGTPSGLVVG
                     DGVRVTGYLQPIAPAAGPGEFDVEAYYRLRYDIRLELVGCDELLRLRSQRVETPAVTA
                     RVRAWLRHRLVRTMPRASPSLTTDLMLSAVAGLQSVQLPRSIEDDFRRAGTIHILVVS
                     GTQVAVLAGVVLALTRGPGPWRWVGACACVPLIAAYTLLVGPEASVSRAALMGVLAAT
                     ALALRRDYDAMTALATAALIIMAADPCSVFRPGFQLSVAASVALVWLGMGRDEVLRPG
                     HLPLRPRRRAGGPWQWVLCGLRVSSVVWLAVTPLLLLHFRQASLTSVVSNLVVVPLAG
                     ALTILGMASAVLVAPLPGLSYVLNYANSILVEGIVRTTAVFANLPWGVWQCGGPFGWG
                     HVALWYVAVVVGHELVHNDRCRRLL"
BASE COUNT          509 a         1300 c         1389 g          675 t
ORIGIN      
        1 ggggtcgcgc gtgctgatcc ccgtgtgagg aggcatccac tatgcgattc cagtcgcgtg
       61 gcatcctcgg gctctgtctg gcgtgtgtca tgacccttcc ggcatacgcc cagaccgtct
      121 ctcttcgcta ccaaccaccg gcgggcatgc gcaccgcata cgacatggag atgtccggtg
      181 agggcaaggt gcaggtcgcg acccaagccg gggcacagga gaccccgctg aacctggttg
      241 ccggcatgcg cgcgtccgag aaggtcctgc aggccggcac cgatggcacg cgcctggttg
      301 agatgcaggt gctggccgcc aacgcgacct acaacggcca gagcgtgccc ctgaacctgg
      361 ccggtcgcac catgcggttc aagcaagatg tgctgggccg ggtctatggc ttcgaggggc
      421 aggctccctc gagcacggcc gtgggtggcg ccgccggctt cgatcccttg tcgctggtga
      481 acgacctgag ccgcgtggtg cggtttccgg agaagcccgt gtctgtgggc cagtcgtggc
      541 gtctggagta ccaggtccgc acaccgtcag gcggcgcccc actggtgtca tgcgagtcga
      601 tcctgcgcca ggtgggcaac gccaacggcc ggccgacggc caccgtggac cacaagctgc
      661 gtgtgcccct gtccatcgag caggggggca tccgcctggt ggggaccctg aacgccaaca
      721 tgcggagcac gtactatacg gactacggta tgctggccgc gtcgcagtgg cgggccgcca
      781 tcaggctgac ggcctacggt gtcggggcga acggagccac cacggtgctg ggctcgtgca
      841 ccatcccgga ccttcgggcc accgtgcgac agtccggctc ctcgtgaggg aggtccgctc
      901 gggcccgcca tggagcttca ccccgaggca cttctggtca cggcaccgag cgtggcactg
      961 gcgctctact acgttccgcg gatgtacctc gatcgcgccc tgaccggatc cgaggcggcc
     1021 gcagccagcg gcattgtgct tggcctgtgt ggtgtatgcc acagcatctg gggggccgcg
     1081 ccgggctatg cgctcggggt ggtcatcatc gcggccatcg gctgcttgcc gtacgctcgc
     1141 cggatgctga gccggcgcga tgagttggct gtggcggaac gcgaactggc ggaatggcgc
     1201 cgaacgatcg cattcgacgc caggaacagg ggcgcctacg tgtacgccgc cgaagcgctc
     1261 cgcaacctgg gtcgctatgc cgaggcggtc agcttgtacg agcgggccat ggccctcggt
     1321 ccccgcgatc cggccgtcca gcgcgccctg gaggtcacca cacagctcaa gcgggcctct
     1381 gccggtgaga actggatctg cgcgacgtgc cgggcggaga acgccgccag gatccgggag
     1441 tgtttccggt gcggggcgca tctgccgctg gcgggagccg ccggcagccc cgtggcggcc
     1501 tacggcatcg cgccgctggc ggccctggtg gttccggctt ccgcactcgg gatctgcggg
     1561 gcgatcagcc cttcggcgat gctgctgctg tgctggctgg gcgcgctggg ggtcgttgtg
     1621 ttctatgcga tcgtgtcacc cgcgagtgag gactagactg gacccatgac cgacgcgttc
     1681 gagccgcgca tccagctgat cctgggactg gggaacccac cccgggagta tgcgggcacg
     1741 cgccacaacg tgggcgccga cgtggtggac cgagtggccg cccgcatggg tatccgcacg
     1801 tggtctcacc gttggcgctc cctgagtgcc gagggccaca tcggcggatg caaagtcacc
     1861 ctcgtcaaac ccatgaccta tatgaaccgc agcggcctgg cggcgcaggc cgcgctgcag
     1921 gaactccgcc tgctgcccag ccagctgctc gtgttggcgg atgacgtgaa cctcgagctc
     1981 ggccggctga ggctgcgacg gaagggaagc gccggtggtc atctggggct cgagtccatc
     2041 gttcagcatc tcggcaccac ggacttcccg cgtttgcgga tcggcgtggg ggggcccgtg
     2101 acgcgcgccc aactcgtcga gcacgtgctg tcgaccctct ccgatgaaga gctcgaagtc
     2161 ctgtctccga ggctcgacga agcggcagac gctgtgtacg attgcgtgcg ggtgggcatt
     2221 gatcatgcaa tgaacgccca taaccggtga gatggcgccc ggggggccat ggccatggtc
     2281 cggcgaacgc tggtgtggct cgcagccggc tatggggccg gggcggtcgc cggcttggcg
     2341 acatggcggg gcctggccgc tgaaggtcca tcgatcgcgg catcagcggc ggtggagcgt
     2401 ctcatgtcgt ggccgtggat cgtccggtcg ctggtggccc ttctgtgtgt gtccaccatc
     2461 gggctgcccc ggctgccgcg gcctggcgtc cgcgcggttc tggcactggc ggccgggctt
     2521 ctggtcggca ccacggccat gctgcggcac gcagtcaccc gctggaccct cccggcgtat
     2581 gttcccgggc agaaggttac gctcacgggc accgccctcg agtcggcagc gcccgggtgg
     2641 ggcctccgcc gagtgccgct ccgggttgcg gagttccgta catcaccagg ggcacggccg
     2701 acggaccgga ccgttctcgc ccacatgggg acgccgtcgg gcctcgtcgt tggggacggt
     2761 gtgagagtga cgggctacct gcagccgatc gcgccggctg ccggccctgg tgaattcgac
     2821 gtcgaggcct actaccgcct gaggtatgac atacgcctcg agctcgtcgg atgcgacgag
     2881 ctgctccggc tgcggagcca gcgtgttgag acgccggcgg tcaccgcgcg cgtccgcgcc
     2941 tggctccgcc accggctggt acgcaccatg ccacgcgcca gcccgagcct cacgacggac
     3001 ctgatgctct ccgcagtggc cggcctgcag tccgttcagc tcccccgctc catcgaggac
     3061 gactttcgcc gtgccggcac gatccatatc ctcgtggtgt cgggcaccca ggttgccgtg
     3121 ctggccggcg tggtgctggc gctgacgcgc ggcccgggac cgtggcgttg ggtgggcgcg
     3181 tgtgcctgcg tgccgctgat cgccgcctac acgctgctcg tgggaccgga ggcatcggtc
     3241 tcgcgcgccg cgctcatggg agtgctggcc gcgacggcat tggcgctgcg ccgcgactac
     3301 gacgccatga ccgccctcgc caccgccgcg ctcatcatca tggccgccga cccctgctcg
     3361 gtgttccgtc ctgggttcca gctttccgtg gcggcatcgg tggcgctggt gtggttgggg
     3421 atgggccgcg acgaggtgct ccgtcctggc cacctgccgc tgcgcccacg gcggcgcgcc
     3481 ggcgggccgt ggcagtgggt cctttgtggg ctgcgcgtgt cgagcgtcgt ctggctcgcc
     3541 gtgacgccac ttctgctgct gcactttcgg caggcatcgc tgacgtcggt ggtgtccaac
     3601 ctggtcgtgg tgcccctcgc gggcgcgctg accatcctgg ggatggcctc ggcggtgctc
     3661 gtggctcccc tgcccggcct ctcctatgtg ctgaactatg ccaacagcat cctggtagaa
     3721 ggtatcgtcc gaaccacggc cgtgttcgct aacctgccat ggggtgtgtg gcagtgcggc
     3781 gggccgttcg gctgggggca tgtggccctc tggtatgtgg ccgtggttgt gggccacgag
     3841 ctcgtccaca acgatcgctg ccgccggcta ctg
//
LOCUS       DAOSPL010000245         3856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_23726_length_3856_cov_2.842410, whole genome shotgun sequence.
ACCESSION   DAOSPL010000245 DAOSPL010000000
VERSION     DAOSPL010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23726_length_3856_cov_2.842410"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PLQ54_05885"
     CDS             <1..714
                     /locus_tag="PLQ54_05885"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS92822.1"
                     /translation="KDPPDGFDFDLWLGPAPEQPFHENIVHYNWHWFWDFGNGDLGNQ
                     GVHQMDVARWALNRGLPTRVHAQGGRLGYKDQGETPNTLNCTFKYDDGTCLVFDVRGL
                     PTNDEGGVRIGNLFYGPEGWMSEGDGFVPHIGYKGDGQAKEITDLPAVGGNGTEDPFV
                     NFIECVRSRRVEDVNCEIEEAHLSAAMCHIANISYRLGGVELAFDPVRERFTGYMADD
                     ANKLLKRKYRKPYVIPDDV"
     gene            902..2311
                     /locus_tag="PLQ54_05890"
     CDS             902..2311
                     /locus_tag="PLQ54_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOS92823.1"
                     /translation="MPAHHSRRLVCAVVSFMLPTLCLAAWVATPGHASPRVRNIVIMI
                     SDGGGYNQAQAASLYRYGRPDALVFQRFPLRLAMSTFSADESGATAGYDPFAAWAEFG
                     YFLRNATDSAAAGTAMACGIKTYNKAIGVDLARQPVENILERAEKLGMATGVVTTVPL
                     SHATPAAFVAHHPDRGAYTHIARQMLMESRADVIIGCGNPLYSDDASLVPEEKRDYQF
                     VGGRETWDALVAGTLGGSRGFEDADGDGTTDYWTLVDTLDSFRSVAAGSAPATRLCGI
                     PRVRSTLQQARGGDRQADPYVVATNTGVPDLALMTQAALAVLGRDRDGFALMVEGGAV
                     DWAAHGHQAGRMIEEELDFARAVEAVVRWVERHSNWDETVLLVTADHETGGLWGPGTG
                     GPEDDQPVFNPLENRGQGRTPGLEWHTGGHTNSLVPLYAIGSAAEGLPAHVVGRDRVR
                     GPYVDNTGLASFLCRRLGR"
     gene            2522..>3856
                     /locus_tag="PLQ54_05895"
     CDS             2522..>3856
                     /locus_tag="PLQ54_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOS92824.1"
                     /translation="MHPLRRYYRFAVLPAAMVAVAVCAISGSPQAGAAASIPTPQNVI
                     IMISDGCGYNQIAVTDLYQYNRLGRQCYESFPVRVAMSTYSTGSGTTPKGYDPSRAWS
                     EFGYVKSNATDSAAAATAMSCGVKTYDAALGLDPYKNRVKHLMERAEEVGKSTGVVTT
                     VEFSHATPAGFVAHNESRNNYLAIAQQMLTQSRTDVIMGCGHPLYTNDAVLGNNETSV
                     KDGQADGHDYQYVGGKAMFDQLAAGTLVASGDYADADGDGVADPWTLVESLSQFVALQ
                     NAPNPPKRVCGVARSYTTLHQARSGDGKAAPYKVKRNSGVPTLAQMTAGALNCLDGDT
                     DGFVLMVEGGAVDWAAHANQPGRLIEEEIDFNDAVDAVVRWIRKNSSFDETLLIVTGD
                     HETGYLWGPGSGGPGNNQPIWNPIAGSGRLQLPGMDWNSGDHTNSLVPLFAAG"
BASE COUNT          685 a         1300 c         1263 g          608 t
ORIGIN      
        1 aaggatccgc ccgacggctt cgatttcgac ctctggctgg gccctgcgcc cgagcagccg
       61 ttccacgaga acatcgtcca ctacaactgg cactggttct gggatttcgg caacggcgac
      121 ctgggtaacc aaggagtcca ccaaatggat gtggcccgtt gggcgctgaa ccgcggcctg
      181 cccacccggg tccacgccca gggcggccgt ctcggctaca aggaccaggg agagacgccg
      241 aacacgctca actgcacctt caaatacgat gacggtacct gcctggtctt tgatgtgcgg
      301 ggcctaccca cgaacgacga gggcggggtg cgcatcggca acctgttcta tggccccgag
      361 ggctggatgt cggagggcga cggcttcgtg ccccacattg gctacaaggg cgacggccag
      421 gccaaggaga tcaccgatct gccagccgtt ggcgggaacg gcaccgagga ccccttcgtg
      481 aactttatcg agtgcgtccg gagccgccga gtcgaggacg tgaactgcga gatcgaggaa
      541 gcgcacctat ccgccgccat gtgccacatc gccaacatct cgtaccgact cgggggcgtc
      601 gagctcgcgt tcgaccccgt gcgggagcgc ttcacgggtt acatggccga cgatgccaac
      661 aagctgctga agcgcaaata ccgcaagccg tacgtcatac ccgacgacgt ctagcttcat
      721 cagactcggg tggtgtatgg tgggcctccg gccgtgcggc gccggaggcg ctgtatctga
      781 cgagggagaa ggcgtgagga cccgttcccg ttgcggtgca gcggaagcgg cggtcctcac
      841 ccgtccttct gcctcgcggt cggttgctcg aaagtctgcc cgcgaggggg aatctgctcg
      901 catgcccgcc catcactcca ggcggctcgt gtgcgccgtc gtgagcttca tgctaccaac
      961 tctgtgcctg gcggcatggg tcgccactcc gggccatgca tcgccgcgcg tccggaacat
     1021 cgtcatcatg atctccgacg ggggcggcta caaccaggct caggcggcaa gcctctaccg
     1081 ctacggacgg cccgacgctc tcgtgttcca gaggttcccg ctgcggctcg ccatgagcac
     1141 cttctcggcc gatgagagcg gcgcgacggc cggctacgac cctttcgcgg cgtgggcgga
     1201 gttcggctac ttcctccgta acgccacgga ctcggccgcc gcgggcacag ccatggcctg
     1261 tggcatcaag acctacaaca aagccatcgg cgtggatctg gcacggcagc ccgtcgagaa
     1321 catcctcgag cgcgccgaga aactcgggat ggcgaccgga gtcgtcacca cggtgcccct
     1381 gtcgcacgcc accccggcgg cgttcgtcgc acaccacccc gatcgcggcg cctacaccca
     1441 catcgcccgg cagatgctta tggagagccg tgccgatgtc atcatcggct gcgggaaccc
     1501 tctgtactcc gacgatgcct ctctcgttcc cgaagagaag cgcgactatc agttcgtggg
     1561 cggacgggag acctgggacg cgctggtggc cggtacgctg ggcgggagcc gcggtttcga
     1621 ggacgccgat ggcgacggca ccacggacta ctggacgctc gtcgataccc tggattcgtt
     1681 ccggagcgtg gcagcaggct ccgcgccggc cacacgcctg tgcgggatcc cacgtgttcg
     1741 ctcaacactc cagcaggccc gcggtggcga ccgccaagcc gacccatatg tggtcgccac
     1801 aaacaccggc gtgcccgacc tcgccctgat gactcaggcc gcactggcag tcctcggccg
     1861 cgaccgtgac gggttcgctc tcatggtcga ggggggcgcc gtcgactggg cggcccacgg
     1921 ccaccaggca gggcgtatga tcgaggagga actcgacttc gcacgcgccg tcgaggccgt
     1981 cgtacgttgg gtcgagcggc acagcaactg ggacgagact gtgctgctcg tcacggccga
     2041 ccacgagacg ggggggctgt ggggcccggg caccgggggc ccagaagacg accagcccgt
     2101 cttcaacccg ctcgagaatc gaggccaggg ccgcactccg ggcctcgagt ggcacacggg
     2161 tggccacacc aacagcctgg tgccgctgta cgccattggc tcggcggccg agggactccc
     2221 cgcccatgtg gttggtcgtg accgcgttcg cgggccatat gtcgacaaca ctggccttgc
     2281 ctcgttcttg tgccggcgct tggggcgcta ggcgggtgct gctgccccgc agtccggcgt
     2341 cggcgccgca cttaacgatg agctaacgca gacctaaccg gggcataacg cgtgttgtgt
     2401 aggctacctc tcgggtgaat gggccggcca ggaacccaac tgtcccggcc ccgcgacgtg
     2461 tcggtcggca gtctccgacc tgagcggggc acttcggacg gaccgagagg agaagtctgc
     2521 cgtgcatccc ctacgcaggt attaccgctt cgccgtgctg cccgccgcca tggtggctgt
     2581 ggctgtgtgc gccatcagcg ggagccccca agccggagcg gccgcgtcga tcccgacgcc
     2641 ccagaacgtg atcatcatga tcagcgacgg gtgcgggtac aaccagatcg cggtcaccga
     2701 tctctatcag tacaaccgtt tgggccgcca gtgttacgag tcgttccccg tgcgggtcgc
     2761 gatgagcacc tattccaccg gcagcggcac cacccccaag ggctatgacc cgtcgcgcgc
     2821 gtggagcgag ttcggctatg tcaagagcaa tgccaccgac tcggccgccg cggcgacagc
     2881 catgtcctgc ggagtcaaga cctatgacgc ggcactcgga ctcgatccct acaagaaccg
     2941 ggtgaagcac ctcatggagc gggccgagga ggtcggcaag tcgaccggcg tcgtgaccac
     3001 ggtggagttc agtcacgcca ccccggcggg ctttgtggct cacaacgaga gccgcaataa
     3061 ctacctcgcc atcgcccagc agatgctcac acagagccgc acggacgtca tcatggggtg
     3121 tggccacccg ctctacacca acgacgccgt actcggaaac aacgagacgt cggtcaaaga
     3181 cggtcaggcc gacggtcatg actatcagta cgtgggcggc aaggccatgt tcgaccagct
     3241 cgctgccggc accctggtgg cctcgggtga ttatgccgac gccgatgggg acggcgtggc
     3301 cgacccctgg actctggtcg agagtctctc acagttcgtg gccctgcaga atgccccgaa
     3361 cccgcccaag cgcgtctgtg gggtggctcg gagctacacc accctacacc aggcccgctc
     3421 cggggatgga aaggccgcgc cctacaaggt gaagcgcaac agcggagtgc ccacgctcgc
     3481 gcagatgacg gcaggcgccc tgaactgcct cgatggcgac acggatggct tcgtgctcat
     3541 ggtcgagggc ggtgccgtag actgggcggc ccacgcgaac cagccaggca ggcttatcga
     3601 ggaagagatc gacttcaacg acgccgtcga cgcggtcgta cgctggatcc gcaagaacag
     3661 cagtttcgac gagacccttc tgatcgtcac cggggaccac gagaccggtt acctctgggg
     3721 cccggggagc ggtgggccag gtaacaacca gcccatctgg aacccgatcg cggggagcgg
     3781 ccgcctgcag ctgccaggca tggactggaa cagcggcgac cacacgaact ccctcgtccc
     3841 gctgttcgcg gccggc
//
LOCUS       DAOSPL010000246         3856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_23727_length_3856_cov_2.528019, whole genome shotgun sequence.
ACCESSION   DAOSPL010000246 DAOSPL010000000
VERSION     DAOSPL010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23727_length_3856_cov_2.528019"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PLQ54_05900"
     CDS             complement(<1..588)
                     /locus_tag="PLQ54_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS92825.1"
                     /translation="MIQADGVSIRLGEFTLTDISFTVATGEYFVILGPTGSGKTVLVE
                     CIVGLHQPSQGRIVLDGRDVTDLLPEKRGVAYVPQDYCLFPHMTVEENIGFGLRIRKA
                     DAAHTARQIQRLSEILQITHLLRRRPVNLSGGEKQRTALARALAVEPTLLLLDEPFAA
                     VDERTREMLCDQFKVVQRELGTTTVHVSHNFEEALS"
     gene            complement(585..1454)
                     /locus_tag="PLQ54_05905"
     CDS             complement(585..1454)
                     /locus_tag="PLQ54_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS92826.1"
                     /translation="MSAPGDAEVLTPASTHRHDRPLRGVLSALLYGYMGLVLAVILAN
                     ALWLGRDNPLTGESYLTSLFANTDLRGQVAFAFRLSLITSLITTMLAMVVGIPSAYAL
                     SRFRFRGMSVIDTVIDLPLVIPPLVAGIALLLFFRQSMAGRWFEQHVVPIVYTQRGIV
                     VAQFFIASAFAIRALKAAFDQVTPRYESVARSLGCSAWQAMWIVALPLARNGIIAGAI
                     MTWARAMGEFAPTMMVAGATPGRTDVLPVAAFLNMSAGNVEAAIAIVVLMIAIAAVTL
                     VTFKRLGGQGYIW"
     gene            complement(1451..2971)
                     /locus_tag="PLQ54_05910"
     CDS             complement(1451..2971)
                     /locus_tag="PLQ54_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOS92827.1"
                     /translation="MRKRTAPLTIALVALALVATLPDVVGCRARTTTGSVTLYARTEL
                     EPALEAIRRAIEKDYPRLSVTTVTKPFHELVEAAKQPSTLGVVIATGAEDLTLLDKAG
                     RLEEGSGITLGEIPLVMVVAPGNPQGLRSPEDVARVGKPLGMVKKSDGGAWATSSESA
                     TQAAGLKGSVAIVAMESGRLVPAVSSGELGAALVGAHMVTPGVEAVPLPGACGQPAGR
                     LLAARLVGTPDDAAYSTLARVLRSQNLRAELVRAVGMRDTSKPDGTGGELLLFCGAGL
                     RLPAEDLITEFKAKTGIAVRPTFTGSGCLLAQITIGETGDLYMPGEDYYMAMAAERGY
                     VTDQRIVAYFVPVIMVQRGNPHNIRGLRDLMKPGVRVGIGEPKACAIGDFTPKVLRAN
                     GISPEEFERNVSAHFATAPELGNAMKIQAVDAVIQWDSLASLYLDAVDVVPIPTDEKT
                     ISPVPLGTLRFSKHPEEAKRFLDFVSGPDGKAIFAKHSYTLDPAHPTFPVAKEDGS"
     gene            3599..>3856
                     /locus_tag="PLQ54_05915"
     CDS             3599..>3856
                     /locus_tag="PLQ54_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92828.1"
                     /translation="MRGPIFNQDDSEFFSVRDPSEVSGEAVDAWVDGLAQAGIGTLMS
                     CVCAMVANYASSVWETRWTRYDPEGPDDQPLLAHLAPESVPA"
BASE COUNT          683 a         1311 c         1262 g          600 t
ORIGIN      
        1 cgagagggcc tcctcgaagt tgtgggagac gtggactgtt gtggtcccca gctcacgctg
       61 aaccaccttg aactggtcac acagcatctc acgagtgcgt tcgtccactg cggcgaatgg
      121 ctcatcgagc aacagcagcg tgggctcgac ggccagcgca cgggccagcg ccgtgcgctg
      181 cttctcaccc ccactcaggt taacgggacg ccgtcgaaga aggtgtgtga tctgcagaat
      241 ctccgagaga cgctggatct gtcgtgcggt gtgcgcggcg tccgccttgc ggatccgcag
      301 gccgaacccg atgttctcct cgacggtcat gtgggggaac aggcagtagt cctgcggcac
      361 ataggccact ccgcgcttct ccgggagcag atccgtcacg tcacggccgt cgagaacgat
      421 ccggccctgg ctcggctggt gcaggcccac aatgcattcg accagcacgg tcttcccgga
      481 cccggttggc ccgaggatca cgaagtactc gccggtagcc accgtgaaac tgatgtccgt
      541 gagcgtgaac tcgcccaggc gaatggagac accgtccgct tggatcacca gatgtacccc
      601 tgtccaccca ggcgcttgaa cgtgaccagg gtcacggccg caatcgcgat catgagcacg
      661 acgatggcaa ttgccgcctc cacgttgccg gcggacatgt tgaggaatgc agccaccggc
      721 agaacgtccg tccggcccgg agtggccccc gcgaccatca tggtgggtgc gaactcgccc
      781 atggcccgcg cccaggtcat gatcgctccc gcgatgatcc cattccgggc cagtggcagc
      841 gccacgatcc acatcgcctg ccatgccgag cagcccaggc tccgcgccac cgactcatag
      901 cgcggagtca cctggtcaaa agccgccttc agcgcacgaa tggcgaatgc cgatgcgatg
      961 aagaactgcg cgaccacaat gcctcgctgg gtgtacacga tgggcacgac gtgctgctcg
     1021 aaccagcggc ccgccatgga ctgacggaag aacagcagca gcgcgatccc cgccaccagc
     1081 ggcggaatca ccagtggcag gtcgatgacg gtgtcgatca cgctcatgcc ccgaaagcga
     1141 aagcgggaga gcgcgtaggc cgaaggaatg cccaccacca tcgcaagcat ggtcgtgatc
     1201 agggaggtga tcaggctcaa ccgaaacgca aacgccacct ggccccgcaa gtccgtgttc
     1261 gcgaagaggc tcgtcagata tgactcgccg gtcaggggat tgtcgcgccc gagccacagg
     1321 gcattggcca ggatgaccgc cagcaccaga cccatgtagc catacaggag agccgacaac
     1381 accccgcgga ggggccggtc gtggcggtgg gtggaggccg gagtcaggac ctccgcatcg
     1441 cccggagcgc tcacgaacca tcctccttcg cgaccgggaa cgtggggtga gccgggtcga
     1501 gcgtgtacga gtgcttcgcg aagatggcct tgccgtctgg cccggacacg aagtcgagga
     1561 aacgcttcgc ttcctccggg tgcttcgaga acctgagtgt accgagaggc accgggctga
     1621 tcgtcttctc gtcggtgggt atgggtacaa catcgactgc atcgaggtag agggaggcca
     1681 gcgagtccca ctggatgacc gcatcgacgg cctggatctt catcgcgttc ccgagctcgg
     1741 gggccgtggc aaaatgggcc gacacattgc gctcgaactc ctcgggcgat atcccgttcg
     1801 ctctgagcac tttgggggtg aagtcgccga tagcacacgc cttgggttcg ccgatgccga
     1861 cacgaacgcc gggcttcatc agatcacgca ggccccgtat gttgtggggg ttaccgcgct
     1921 ggaccatgat cacgggcacg aagtaggcca cgatccgctg gtcggtgacg taaccccgct
     1981 ccgccgccat ggccatgtag tagtcctcgc ctggcatgta gaggtccccg gtctcgccga
     2041 tggtgatctg ggcgagcaga cagcctgatc cggtgaacgt gggccgcacc gcgatgcccg
     2101 tctttgcttt gaactccgtg atgaggtcct ctgcgggcaa gcggaggccg gcgccgcaaa
     2161 agagcagtag ctcgccgccc gtgccatccg gcttggatgt gtcgcgcatg cccacggctc
     2221 tcacgagttc tgcacgcagg ttctgactac ggagcacacg agccagcgtt gagtaggcgg
     2281 catcgtcagg ggttcccacg agccgagccg ccaggagtct ccctgccggc tgcccgcacg
     2341 ccccgggcag gggcacggcc tcgacgccgg gcgtgaccat gtgcgcgccc acgagtgccg
     2401 ccccaagttc ccccgacgag acagccggaa caagtcggcc cgactccatg gcaacgatgg
     2461 cgaccgagcc cttgaggccc gcggcctgcg tggcgctctc cgagctcgtg gcccaggcgc
     2521 ccccgtcgct cttcttgacc atgccaaggg gtttcccgac ccgcgccacg tcctcgggtg
     2581 accggaggcc ctgtgggttc ccgggggcca ccaccatgac gagaggtatc tcgccgagcg
     2641 tgatcccgct gccctcctcc aggcgccccg ccttgtcgag cagcgtgagg tcttcggctc
     2701 ccgtcgcgat aacgactccc agtgtgctgg gctgcttcgc ggcttcgacc agttcgtgga
     2761 agggcttggt caccgtcgtg acactgaggc gtgggtagtc cttctcgatg gcacgccgga
     2821 tcgcctcgag ggccggctcg agctcagttc tggcatagag ggtcaccgat ccggtcgtgg
     2881 tccgagctcg gcaacccaca acgtccggca aagtggccac gagggccaac gccaccagtg
     2941 cgatggtcag cggcgcagtc cgcttcctca caggccgcgc ggagcccctc tcgtcgctcc
     3001 cagacactgc aaccccccca cggtacagac caccggccac cgagccaccg cgtggaaacc
     3061 gtcagccaag gcgccttcgg aagatgggca ccgcgtcggc ggatccaggc ggcgcagacc
     3121 ggggaacggc catggccgac ccggtgagtg tacgcgcatg cgctacctgc ggcaactgca
     3181 ctcgccgcgg tcggccggag tgatcccatc agacggaggc ctggcctcga cagccgcggc
     3241 ccggacgcac caagacctag gtaccaattc gcgcgtggag gctcctttgt gacaccaatc
     3301 tgtaggggcg acggcgggac ggcaggccaa gcgcccaatg tatggggggg ccagcaggcg
     3361 ccgccggcac gggggcggaa gggaacccgg ccggggtgcg tgccctggcc ccggggccgc
     3421 cccgggttca ctcaccgatt ggagccgcgc ggcgccgccg ggagcctgtg gcccgcgcac
     3481 cacgcccatg acacgtcgcg aatcgctcaa gaccgttgct ctggctgcag ccagcgcgtg
     3541 ggccggtggc ccatcggcct ccgccgagtc cagcccgaag tactcaggag ggaacccctt
     3601 gcgcggcccc atcttcaacc aggatgacag cgagttcttc tccgtccgcg acccgtcgga
     3661 ggtcagcggc gaggctgtcg acgcctgggt cgacgggctt gcccaagccg gcattggcac
     3721 cctcatgtcg tgcgtgtgcg ccatggtggc caactacgcc agcagtgtgt gggagactcg
     3781 ctggacccgc tacgacccag aaggccccga cgatcagcca ctgctcgcac acctggcgcc
     3841 cgagagcgtg ccggcg
//
LOCUS       DAOSPL010000247         3852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_23750_length_3852_cov_2.942850, whole genome shotgun sequence.
ACCESSION   DAOSPL010000247 DAOSPL010000000
VERSION     DAOSPL010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23750_length_3852_cov_2.942850"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..2592
                     /locus_tag="PLQ54_05920"
     CDS             298..2592
                     /locus_tag="PLQ54_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92829.1"
                     /translation="MESGSTRREFIQAAGVVVMGVVAGSGTAKAREELVLATTELSLS
                     LDHTGRLISLLDRAGGRELLPAGVTPPLLRLVFADRQLAPVSMTRAERESQLALEFEG
                     GISATVVVAVQPSHLTFRLEVLEGERPEQAHWGPFPCTTADTIGETVGVVRDSQIAVG
                     IQSLGIETIGGARAEGGGSALFAYAIEHDGGVVGSAIALFACPATDALARIGEIEVAE
                     GLPHPMLDGEWGKVSRTAKLSYLISPFGEQTIGAVLDRAEAAGLTYVYHPGPFLNWGH
                     FELNQVEFPEGDASMNRCVETAAERGVRLGVHTLTGFITTNDPYVSPVPDSRLARWGS
                     STLTAAIDAVATELPIADPASFRERGTLGAAIIGPELVQYGSVSDTEPWRLLDCARGA
                     FGTMAAAHEAGADIGHLADHAYRTLYPGIDNGLMDELTDRLVRLFNDTGLRQISFDGL
                     EGLSTYGYGEYARNRFVKQCYDGWQGEVISDASNLLHYLWHIHTRMNWGEPWGKATRD
                     GMAEYRFANQDYFERNLWPKMLGWFQLRLAGDGVEATSLDDIEWVLSKAAAYDAGFAL
                     VSSLAEMDANGQTDAILAAVREWETARLAGAFSAEQRERLRGAGEWHLEPAGEGRWQL
                     VPLVFASLAHTPQAGQPGQPMESVWDVTNPFAEQPLWFALQASPTAVGPLQDPSLQVA
                     GRTVTFRCSLSAGRYLVCQGERAATICDANWKPVGTVEADTPPPMLRSGAQKVTFSSS
                     PMAGPSPAAKLRIRMAGEPEMVGR"
     gene            complement(2817..>3852)
                     /locus_tag="PLQ54_05925"
     CDS             complement(2817..>3852)
                     /locus_tag="PLQ54_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92830.1"
                     /translation="SVILAQVQAPLRREGDRALVDFATSDPFVDAYREAGFTDPLVYN
                     PFHDRLATLLLEQFGLAERFPSIENYGETIRVFGEDEYPPVLHEIYRQVVRDIRNHAR
                     EANWPPMLFYPVDEPNELGDWRIPAARLEYRLTREAVPGLRTFCTAYSVPAMERLDPW
                     LDVRTVPISSLIADERTNTEFRDYLRRAGGEIWGIEWPAMWDDFWRAREIAGFLPAKA
                     GVTGMTSWTYYTPAPVADEYDDLRGEFKHCLFVYRDTDGSLVPTVTWEGIRAGATDWR
                     YFATLEEEAARATGSERDRAMRALHEVRDAVPWSVDGREGWGDDRATQLRAKLAEAIA
                     RLQRRAAREG"
BASE COUNT          603 a         1314 c         1343 g          592 t
ORIGIN      
        1 gcggcccctc cgaaagacca ggcgggcggt ccgcgcgacc ttcgcgagac ccagctggtc
       61 ggccccaggc cggccgtacc ggtcggctgt gaggtgtgga gtgggacgct ccacctacgc
      121 gccgttcggg cgcacccctg tgcggccccg ccaccagaca gggcaggtcg ttgtacgggc
      181 cgtcgcgcgc accgccctgg cccaagctgc cgccagtgcc ctgcgaggac taccagggcc
      241 gctgccgaaa ccctctgctg cctcaccgga cccgacagcg gcgaagaggg tgcccatatg
      301 gaatccggct cgacacgcag ggagttcatt caggcggcgg gggtggtggt catgggcgtc
      361 gttgcaggct cgggcacggc caaggcccgg gaggaactgg tgctcgccac aaccgagctc
      421 tctctcagcc tggaccacac cgggcgcctg atcagcctcc tcgaccgcgc tggcggccgc
      481 gagcttctgc ctgcgggcgt cacaccgccc ctgctgcgac tggtgttcgc agaccgccag
      541 ctcgcaccgg tctcgatgac tcgagccgag cgagagagcc agctggcgct ggagttcgag
      601 ggcggcatca gcgcgacggt ggtcgtcgcg gtccagccca gtcatctgac gttccgcctg
      661 gaggtactcg agggcgagag acccgagcag gcgcactggg ggccgtttcc ctgcacgacc
      721 gcggatacca tcggcgagac ggtgggtgtg gtccgcgaca gccagatcgc tgtcggcatc
      781 cagagtctcg gcatcgagac catcggcggc gcgcgagccg aagggggcgg cagcgcgcta
      841 ttcgcctacg ccatcgagca cgacggcggg gtggtcggat ccgccatcgc cctgtttgcg
      901 tgcccggcga ccgatgcgtt ggcgcggatc ggcgagatag aagtcgccga gggcctcccc
      961 caccccatgc tcgatggcga atggggcaag gtgtcgcgga ccgccaagct ttcgtatctg
     1021 atctcgccct tcggcgagca gaccatcggg gcggtgctcg atcgggccga ggctgccggg
     1081 ctgacctatg tctaccaccc gggaccgttc ctcaactggg ggcacttcga gctcaaccag
     1141 gtcgagttcc cggaaggcga tgcaagcatg aaccgctgtg tcgagaccgc ggcggagcgc
     1201 ggggtccggc tcggggtgca cacgctgacg ggtttcatca cgaccaacga cccctatgtg
     1261 tcccccgtgc ccgattcgcg tctcgcccgg tggggctcgt cgacgctgac ggcggccatc
     1321 gacgccgtgg ccacggagct gcccatcgcc gacccggcgt ccttccgtga gaggggcacg
     1381 ctcggggcgg cgatcatcgg cccggagctc gtgcagtacg gctcggtctc ggacaccgag
     1441 ccgtggcggc tactcgactg cgcgcgcgga gcgttcggga ccatggcagc cgcgcacgaa
     1501 gcgggagcgg acatcggcca cctggccgac cacgcatacc gcacgctcta cccgggcatc
     1561 gacaacggct tgatggacga gctgaccgac cggctcgtgc ggttgttcaa cgacaccggg
     1621 ctaaggcaga tctcgttcga tggcctcgag gggctctcga cctacggcta cggcgagtac
     1681 gcgcggaacc ggttcgtgaa gcagtgctac gacggctggc agggcgaggt catcagcgac
     1741 gccagcaatc ttctgcacta tctgtggcac atccatacgc ggatgaactg gggcgagccg
     1801 tggggcaagg cgacgcgcga cggcatggcg gagtaccgat tcgccaacca ggactacttc
     1861 gagcgcaacc tgtggccgaa gatgctcggg tggttccagc tcaggttggc gggcgatggc
     1921 gtcgaggcca cctcgctgga tgacatcgag tgggtgctgt cgaaggcggc cgcttatgac
     1981 gccggattcg ctttggtcag cagcctcgcc gagatggatg ccaacgggca gaccgacgcc
     2041 atcctcgcgg ccgtccgcga atgggaaact gcccgcctgg ccggagcctt cagcgccgag
     2101 cagcgggagc ggctccgcgg ggcgggcgag tggcacctcg aaccggcggg cgagggccga
     2161 tggcagctcg tgccgctggt gtttgcctcg ttggcgcaca cgccgcaggc gggccagccg
     2221 gggcagccga tggagtcggt gtgggacgtc acgaacccgt tcgccgagca gccgttgtgg
     2281 ttcgcactcc aggcctcgcc cacggccgtc gggccgttgc aggacccgtc gctgcaggtc
     2341 gccgggcgaa cggtgacctt tcgctgcagc ctgagcgcgg gccggtatct ggtgtgccag
     2401 ggcgagcgcg cggccacgat ctgcgatgcg aactggaagc cagtgggcac ggtggaggcc
     2461 gacaccccgc cgcccatgct tcgttcgggc gcgcagaagg tcacgttttc gtcgtcgccg
     2521 atggcagggc catcgccggc ggcgaagctg cggattcgga tggcgggtga gcccgagatg
     2581 gtgggcaggt agggaggtgg ccgactccgc ggagatcggg ttgggtgcgg cggggagcag
     2641 gtgatcggag ccattgtctg ctgccgcgct tgctccgctc ttgtccacga aagacgcgaa
     2701 acacacgaaa ggaaccccga cacccaccaa gccctgccct gtcgctccct atcctctctc
     2761 gtgtctttcg cgggcatcca cgggaactcc gcccaagccc cggggcgccc cggaggtcac
     2821 ccctcccgcg ccgcccgcct ctgcaaacgc gcgatggcct cagccagctt cgcccggagt
     2881 tgcgtggcgc ggtcgtcgcc ccacccctcc cggccgtcga cgctccacgg cacagcatcg
     2941 cgcacctcgt gaagcgctcg catcgcgcga tcacgttccg accccgtggc ccgcgcggct
     3001 tcctcctcga gcgtcgcgaa gtaccgccag tcagtggcgc cggcccggat gccctcccag
     3061 gtcacggtcg gaacgaggct cccatcggtg tcacggtaga cgaacaggca gtgcttgaac
     3121 tccccacgca ggtcatcgta ctcatccgcc acaggcgccg gggtgtagta ggtccaggat
     3181 gtcatcccgg tcacacctgc cttcgcgggc aggaacccgg cgatctcccg tgcgcgccag
     3241 aagtcgtccc acatggccgg ccactcgatc ccccagatct ctccgccagc gcgacggagg
     3301 tagtcgcgga actcagtgtt cgtcctctca tcggcgatga gtgagctgat ggggaccgtc
     3361 cgcacgtcga gccacggatc gagccgctcc atcgcgggaa ctgagtacgc cgtgcagaac
     3421 gtgcgcagcc ctggcaccgc ctccctagtg aggcggtact cgaggcgcgc ggcagggatg
     3481 cgccagtcac cgagctcgtt cggctcgtcc accgggtaga acagcatcgg cggccagttg
     3541 gcttcccgtg cgtggttgcg gatgtcccgc acaacctgcc ggtagatctc gtgcaggacc
     3601 ggcgggtact cgtcctcgcc gaacacgcgg atcgtctcgc cgtagttctc gatcgacggg
     3661 aagcgctcgg ccagcccgaa ctgctcgagc aagagcgttg ctagccggtc gtggaacggg
     3721 ttgtacacca ggggatcggt gaagccggcc tcgcgatacg catcgacgaa cgggtccgat
     3781 gtggcgaagt ccacgagggc gcggtcgccc tcgcgccgca gtggagcctg cacctgggcc
     3841 aggatcaccg ag
//
LOCUS       DAOSPL010000248         3848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_23776_length_3848_cov_3.292381, whole genome shotgun sequence.
ACCESSION   DAOSPL010000248 DAOSPL010000000
VERSION     DAOSPL010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23776_length_3848_cov_3.292381"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLQ54_05930"
     CDS             complement(<1..273)
                     /locus_tag="PLQ54_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92831.1"
                     /translation="MRGPRLLLQCGLCAVGVGATAAAVWLAMPMSTRTTPAEFSRAFI
                     TCAAVAITLVVGFVAPGATAGAFTLERERQTLEALLVCPLPRRSLVL"
     gene            complement(830..2911)
                     /locus_tag="PLQ54_05935"
     CDS             complement(830..2911)
                     /locus_tag="PLQ54_05935"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOS92832.1"
                     /translation="MGDVRSRDRRMLAGSFLAALMAHVGLALCVTVARPTAAPERPEP
                     IQVADVSIIEPPKPPAPPVVRSRPVVRRRVVVRRIVRRPAPSKPVAVVSLAPRPDVRP
                     QSMPETAPAPSAAPADTRPVEVAAAPEAPAETPPELAPDRLEGPSNTATDAPSAVPRP
                     SPEPAPEGAIKLAKAEPAWARESLMPEPPSPVDRPSPVAGTLEEPEGRGEVPVASQPE
                     GPSGAPGELGTPGDGPLQVAMDTSGLAPGGGGGPSSLPGAPGEGQGGPSGLQRMVIEF
                     ADVDLGFDSGGPGGLGQGAGGGEGGGIGEGRGTGSGDGSGDGGGPVAFAWTGGGGGGA
                     GLGGPGTGPGMGADGNGTGWFTIGVPGGTSGGMPGAMGGGGGGGPILGKLDPGGLGDG
                     VPGALGWGLGGRGGPGLGGGAGGGTGGSGTGPGNAPLWTAGLPGGSGGSGFGDSLGLP
                     GGLGGLGGLGAGGGAGMGGLWGTGVGSAPGTSLALGGNLGQGQVASLIPQATGRFTMV
                     VPRTNTDYDANPMATRNLLQEVSKRTLIPATVEERFVPLEWEAIKDAPVVYLSGHHPV
                     AFTDEQRRMLRRYVEEGGTILAEGGGPLFDMTFQKEVQAIFGAAPQKIGPQHQIYQSY
                     YDMHRTGDDETLRGVELGGRLGVIYTDRPLGAAWVGSDSTITPEQREKAFRTGINVYQ
                     YVVENWIKRRP"
     gene            complement(2996..3400)
                     /locus_tag="PLQ54_05940"
     CDS             complement(2996..3400)
                     /locus_tag="PLQ54_05940"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOS92833.1"
                     /translation="MRRERFSRLRPEGGVPVISMADIAFLLILFFLLTSSMTKETGVD
                     VKLPRAQQADMLPKRDISIEVSKSGDIQLNKRAVSLAQLPAALHAELGETSSKKVTIR
                     GDQDSPYGTMVQVMAAVRGEKAEILLAAEMER"
     gene            complement(3390..>3848)
                     /locus_tag="PLQ54_05945"
     CDS             complement(3390..>3848)
                     /locus_tag="PLQ54_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOS92834.1"
                     /translation="GRTEAAIVAAAEKSPVAIVARAGLECANGSAGKTREAMDECARL
                     QVSRVEQYLSLLSTVAYIAPLLGFLGTVTGMINAFEQISMTNEVTPRLVAAGVSEALI
                     TTAFGLMIAIPTYAAYNLFVARVASIENDIETVTSHIIAELHPEALSVAA"
BASE COUNT          534 a         1368 c         1365 g          581 t
ORIGIN      
        1 gagcaccagg ctgcgccgag ggagcgggca gactagcagg gcctcgagcg tctggcgctc
       61 gcgctcgagg gtgaaagctc cagccgtggc cccgggggca acgaacccga cgacgagggt
      121 gatggcgacc gcggcgcatg tgatgaacgc tcggctgaac tcggccggtg tcgtgcgcgt
      181 gctcatcggc atcgcgagcc agactgcggc ggcggtggcg ccaacgccca cggcgcacaa
      241 cccgcattgc agtagcagcc gcggtccgcg catgcgcgac ctcagctcgc ggacgaacac
      301 cgggttcaga tcgatggcac gcgtggtgtg tacccgcctc tccggtggcc ggggatggga
      361 gcctctcccg cgcaagggtg cggcggggag cgctcccacg cgcctcgcgc ttcggccggc
      421 tagcggccga tcgaggccgg tcgtgatgtg ccctcggctc ccccttcaga ttcacgtcct
      481 cgaacagtca gtgagagccc gacccgactc cgatgatgat aaccgccggt gccccgtctt
      541 tgggcaacgt gagccgcacg cgtcaaaggg cacattgggg ctggagccgc cgaggtggcg
      601 gcagtaggcc gagagcggac aggtcgcgcc gatcgtggcg gtcatcgacc tggaggtcac
      661 gcccagctct cccgggaagc cgtcggaggg agtccagccg ggagcgccct cctcggccct
      721 gccgctctcc acgcacgggt cgaggctgag cgtccacgtg ccacaccacc gttgcatgct
      781 cggcaaccaa cggcggcgcg ttggcggaac cggcgttctg cgctccggtc tacggtcttc
      841 gcttgatcca gttctcgacg acgtactggt agacgttgat gcccgtgcgg aaggccttct
      901 cgcgctgctc gggggtgatg gtagagtcgc tacccaccca ggcggcgccc agaggcctgt
      961 cggtgtagat cacgcccagg cggcccccaa gctcgacgcc gcgcagcgtc tcgtcgtctc
     1021 cggtccggtg catgtcgtag tagctctggt agatctgatg ctgtgggccg atcttctggg
     1081 gtgcggcacc gaagatggcc tggacctcct tctggaaggt catgtcgaac agcggcccgc
     1141 cgccctcggc gaggatcgtg ccgccctcct cgacatagcg cctcagcatc cggcgctgct
     1201 cgtccgtgaa cgccacgggg tgatggcccg acaggtatac gacgggtgcg tccttgatgg
     1261 cctcccactc gagcgggacg aaccgctctt cgaccgtggc cgggatcagc gtgcgtttgg
     1321 acacctcctg cagaaggttc cgcgtggcca tgggattggc gtcgtagtcg gtgttggtgc
     1381 ggggcacgac catggtgaac cggcccgtgg cctggggtat gagcgaggca acctggccct
     1441 gaccaaggtt ccctcccaga gcgaggctgg ttccgggtgc ggagccgaca ccggtacccc
     1501 agaggccgcc catgcccgcg cctcctcctg ctccgagccc gccgagcccg ccgagcccgc
     1561 cgggcaggcc gaggctgtcg ccgaagccgc tgccgccact gccgccgggg agtccggcgg
     1621 tccatagtgg cgcgttcccc ggccccgtgc cgctgccgcc ggtaccgccg cctgcgcctc
     1681 cgccgaggcc tggcccgcca cggccgccga ggccccaccc gagggcaccg ggcacaccat
     1741 cgcccaggcc gcccggatcc agcttcccaa ggatcggccc accgccccct ccgccaccca
     1801 tcgcgccggg cattccaccg ctcgtcccac cgggcacgcc gatggtgaac caccccgtgc
     1861 cgttgccgtc ggcgcccatg ccgggtccgg tgcctgggcc gcccaggccc gcgccgccac
     1921 ctccgccgcc agtccatgcg aaagctaccg gcccgccgcc atcgcccgaa ccatcgcccg
     1981 aacccgtgcc gcgcccttcg ccgatgccgc ccccctcgcc cccgccggcg ccctggccga
     2041 ggccgcccgg cccgccggag tcgaagccca ggtccacatc ggcgaactcg atcaccatcc
     2101 gctggaggcc gctcggtccc ccttggcctt cacccggggc cccgggcagt gaactcggcc
     2161 ctccgccccc gcccggcgcc aggccggagg tatccatggc gacctggagc ggcccgtcac
     2221 cgggtgtgcc caactcgccg ggcgcgcccg atgggccctc gggctgggag gccaccggca
     2281 cctccccccg gccttccggc tcctcgaggg tccccgcgac agggctcggc cggtcgaccg
     2341 gggagggggg ctcaggcatg agcgactctc tggcccaggc tggctcggcc ttggcgagct
     2401 tgatggcccc ttcgggcgcc ggctcggggg atgggcgcgg aaccgcagag ggcgcgtcag
     2461 ttgccgtgtt ggaggggccc tcgagccggt caggggcgag ttcgggcggc gtctccgctg
     2521 gggcttctgg ggccgccgct acctcgacgg gtctcgtgtc cgcaggggcg gcgctcggcg
     2581 ccggggccgt ctcgggcatg gactgcggcc gcacgtcggg gcgcggagcc aacgagacca
     2641 cggccacggg cttgctcggc gcggggcggc ggacgatgcg gcgcacaacc acccgacggc
     2701 gcacaacggg gcggcttctg acgactggcg gagcgggggg cttggggggc tcgatgatgc
     2761 tcacatcggc gacctggatc ggctcggggc gttccggcgc ggcggtcggc cgcgcgacgg
     2821 tcacgcacag ggccaggccc acgtgggcca tcagggccgc caggaagctg ccggccagca
     2881 tgcgccggtc tcgcgagcgg acatcgccca tagccgtact cggctcctcc atccagtggg
     2941 cccggacccg ggcccccgtc tcagccctct tcccatggcg gacggggcac gatgcctacc
     3001 gttccatctc ggcggcgagg aggatctcgg ccttctctcc gcggacggcg gccatgacct
     3061 ggaccatggt gccgtagggg gagtcctggt cgccgcgaat ggtgaccttc ttgctcgatg
     3121 tctcgcccag ctcggcatgg agcgcggcgg ggagctgggc gaggctcacc gcacgcttgt
     3181 tcagctgtat gtcgccggac ttggagacct cgatcgagat gtcacgcttc gggagcatgt
     3241 ctgcctgctg ggcacgcggc agtttgacgt cgactcccgt ctccttggtc atgctgctgg
     3301 tcaggaggaa gaagaggatc agcaggaatg cgatgtcggc catggagatc acggggacac
     3361 cgccctcggg ccgaagtcgg ctgaagcgtt cacgccgcaa cgctcaacgc ctccgggtgc
     3421 agctcagcga tgatgtgtga cgtgacggtc tcgatatcgt tctcgatgga tgccactcgg
     3481 gccacgaaca ggttgtaggc ggcataggtc gggatggcga tcatgaggcc aaaggccgtg
     3541 gtgatgagcg cttcggagac gccggcagcc accagcctgg gggtgacctc gttcgtcatg
     3601 ctgatctgct cgaaggcgtt gatcataccc gtgacggtgc cgaggaaccc gagcaggggt
     3661 gcaatgtagg ccacggtcga gagtagggag aggtactgct cgacccgaga gacctgcagg
     3721 cgcgcgcact catccatggc ttcgcgcgtc tttccggcgg acccgttggc acattcgagg
     3781 ccggcgcggg cgacgatggc gacggggctc ttctcggcgg cggccacgat ggcggcttca
     3841 gtgcgtcc
//
LOCUS       DAOSPL010000249         3847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_23783_length_3847_cov_4.865243, whole genome shotgun sequence.
ACCESSION   DAOSPL010000249 DAOSPL010000000
VERSION     DAOSPL010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23783_length_3847_cov_4.865243"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PLQ54_05950"
     CDS             complement(<1..515)
                     /locus_tag="PLQ54_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92835.1"
                     /translation="MTDENRLREAFLAKQRHLLAALEVVPEFTPHGTTMGDDSEANWA
                     RVLREFLPGRYGVAKGHVMDSRGTTSDQIDLLVYDAQYTPLLAQTSSGDLFVPAEAVY
                     AVFEVKQEMNKTFMDYAGTKIASVRRLHRTSVPIAHAGGVYPPKRPIAILGGLLTTRS
                     GWADLQGEAAV"
     gene            complement(967..2478)
                     /locus_tag="PLQ54_05955"
     CDS             complement(967..2478)
                     /locus_tag="PLQ54_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOS92836.1"
                     /translation="MNDAATEFVEAAVRRYDEELNRAGFRTTRCGESPADWSWVGHVE
                     PEHEELTVSLKEGFPFAAPRVALPARAGTADWHQTADGVLCLWDTHAQGDLPWLDAAE
                     LLGRVQQWIRNAADGWVADAPQLDLEAYNSPRVITRRGQSVLPILIIDRWADLADHWF
                     RATMPTDTGLMELKGYPRRTPPATSSVRSRRRRRGRGRPDRFVNGIAVDLGEMTHPLI
                     PTDDLLDALGVQTPTVGGLLTAGRPVLVAARYSRAGVHGLMGFWLEPQGDGVIRGCLP
                     VVEREASQHRRAGWHASSIADRTVTVIGAGSIGSYVADLLHRSGVHDLHVHDSDLLLP
                     GNLVRHAASAAQVGAMKADAVRDTAAQRDPSWPITARPRINSLAEAVELLRDRDLVVD
                     CTGDRRIWHLMRAAADIAGKSFVHVAVMGHGQYGRVDICPPLDGAEPLDEDPVVGVAL
                     TEWEGGCGDPVSPTPPIAVLETAAMGARFALRMLAGEQVPPAGESRALFPDVA"
     gene            complement(2475..>2858)
                     /locus_tag="PLQ54_05960"
     CDS             complement(2475..>2858)
                     /locus_tag="PLQ54_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92837.1"
                     /translation="DAPGSTAEYYTVALETMAPILRAHAQGSEPLMNPALPNQQLHVR
                     ATQVELDAVAEAWEQAAVDARAALETDDEQQAARTFLRLLGQNSDGDDVFTVPAASAA
                     ATQSAAAAVAAGHRKLPSGDSPNFA"
     assembly_gap    2859..2868
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2869..3470)
                     /locus_tag="PLQ54_05965"
     CDS             complement(<2869..3470)
                     /locus_tag="PLQ54_05965"
                     /inference="COORDINATES: protein motif:HMM:NF037736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOS92838.1"
                     /translation="MAILSKQFKDALSMIEPGVDAAHAAEAHTEVRDYLERDAELSDW
                     GLHTVLIGSYKREVSIRRVKDVDVFCELEDLPEDADPQELLDKFVDLLTDEYDDRVCK
                     NDRSVKVEFPDYDMHVDLVPARYVSGVWEIPDKDGGWEATHPIRFGELTTSRNGDHDG
                     HYVPIVKLMRQTRRALLGDAKPGGLFIEVAAYHAFADIPNA"
BASE COUNT          614 a         1273 c         1257 g          693 t
ORIGIN      
        1 ttaacagccg cttccccctg caggtcggcc catccgctcc tggtcgtgag gaggccacct
       61 agaatggcga tgggccgttt tggcggatag accccgccag catgggcgat gggcacactg
      121 gtgcggtgga gtctcctgac actcgcgatc ttggttcctg cgtagtccat gaacgtcttg
      181 ttcatttcct gcttcacctc gaagaccgcg tacacagctt cggcggggac gaacaggtca
      241 ccgctactgg tctgggcgag aagaggcgtg tactgcgcgt catagacaag caggtcgatc
      301 tggtcactgg tggttccgcg cgaatccatg acgtgccctt tggcgacccc gtaacgaccg
      361 ggcaggaact cccgcaagac tcgtgcccag ttcgcctccg agtcgtcgcc catcgtggtg
      421 ccgtggggcg tgaactccgg cacgacctct agcgccgcaa gcagatgtcg ctgtttggcg
      481 aggaaggcct ctctgaggcg gttctcgtct gtcatggtgc gactccttcc acggctacgc
      541 gaatcgtgtg caagtcgcct tcggctgtca cgttccgggc gtggccgacc cagccgctag
      601 ggccgagagc cattaccagt agcgccacgt cgtgccctcc agcttgggca agtgcgcgca
      661 cagcatcgat gtccgtcccg ctcggtccga tgggcaaggg atggctgtgc cattcgccaa
      721 cgtagccggt cagtcctgct tggtcacggt gcacgtgctc gtcgaggatt tgcttggctg
      781 ccgtcgcgtc gcgcctgtag cgaacgcgtg tcgcccgaga gtcccggacc acgggtgcgg
      841 cgaggacaag tggtccaccc acggtgtagt agccaaggag taggccgccc acctctcgtg
      901 gaagggcacg gctgctggag cgagttaccg ctcgccaagc cggtcgggtg aacgtgagtg
      961 agctcgtcat gctacgtccg ggaacaatgc cctggattcg ccggctggtg gcacctgctc
     1021 accggcgagc attcgtagag cgaagcgggc acccatggca gcggtctcca gcacggcgat
     1081 cggaggcgtg ggactgactg ggtcaccaca acctccttcc cactcggtga gcgctacgcc
     1141 cacaactgga tcctcgtcga gcggttcggc gccgtcgagc ggggggcaaa tgtcgacccg
     1201 gccgtactgg ccgtggccca ttacggcgac gtgcacgaac gactttcccg cgatgtcggc
     1261 ggcggcgcgc atgaggtgcc atatgcgcct atcgccggtg cagtccacga ccaagtcgcg
     1321 gtcacgcagg agctccaccg cctcagcgag gctgttgatt cgagggcgcg ccgtgatcgg
     1381 ccacgatggg tccctttggg cggcggtatc tctcaccgca tcggccttca tggcgcctac
     1441 ctgtgcggcc gatgccgcgt gacgtacgag gtttccgggt aaaagcaggt ccgagtcgtg
     1501 cacgtggaga tcgtgaacgc cgctgcggtg tagcagatcg gcgacatagg acccgatact
     1561 gccggcaccg atcaccgtga ccgttcggtc agcgatggac gaggcgtgcc agccagctcg
     1621 acggtgctgt gatgcctcgc gctccacgac gggcaggcac ccgcggatga ccccgtcccc
     1681 ttgtggttcg agccagaagc ccatgaggcc atgcacgcca gcgcggctgt aacgcgcggc
     1741 gaccagcacg ggtcggcctg ccgtcaggag gcccccgaca gttggcgtct gtacgccaag
     1801 ggcgtccagg agatcgtcgg tcgggatcag ggggtgcgtc atctcgccca ggtcgaccgc
     1861 gatgccgttg acgaacctgt cgggtctacc ccgccccctc cgtcgcctgc gcgaacggac
     1921 gcttgaagtc gctggaggag tcctgcgcgg ataccccttc aactccataa gacccgtgtc
     1981 cgtcggcatg gtggcgcgga accaatggtc ggcgaggtcg gcccacctgt cgatgatcag
     2041 tattggcagg acactctggc ctcttcgagt aatgacgcgg gggctgttgt acgcctctag
     2101 gtcgagctgg ggcgcatccg cgacccaccc gtcagccgcg ttccggatcc actgctggac
     2161 gcgcccgagg agctcggccg cgtccagcca cgggaggtcg ccctgggcgt gtgtgtccca
     2221 cagacagagg acaccgtccg cagtctggtg ccagtcggcg gttccggcac gggctggcag
     2281 cgccaccctt ggggccgcga aaggaaagcc ttccttcagg ctgacggtca gctcttcgtg
     2341 ctcgggctcc acgtgcccga cccacgacca gtccgcgggc gactccccgc agcgagtcgt
     2401 ccggaatcct gcccggttga gctcctcgtc gtagcggcgg acagcagcct cgacgaactc
     2461 ggtcgcagcg tcgttcatgc gaagttggga gaatccccgc tgggaagctt gcggtggccg
     2521 gccgccactg cagcggccgc agactgcgtc gcagcggcag aggcggctgg gaccgtgaac
     2581 acatcgtcgc cgtcgctgtt ctgtcccagc aggcgcagga acgtccgggc ggcctgctgc
     2641 tcatcatcag tctcaagagc tgcgcgggcg tcgacggcag cctgctccca ggcctcggcg
     2701 accgcgtcca actcgacctg cgtggcgcgt acatgtagtt gttggttcgg cagtgctggg
     2761 ttcatcaacg gctctgaccc ctgggcgtgt gcgcgcagaa tgggcgccat cgtctccagg
     2821 gccaccgtgt agtactcagc ggtcgagcca ggagcatcnn nnnnnnnngc gttcgggatg
     2881 tcggcgaagg cgtggtaggc ggcaacttcg atgaaaaggc ctcccggctt ggcgtccccc
     2941 agcagggctc gccgtgtctg cctcatgagc ttgacgatcg ggacgtagtg cccgtcgtga
     3001 tcgccgttcc gtgaagtggt gagttcaccg aacctgatcg gatgcgttgc ctcccaccct
     3061 ccgtccttgt cggggatctc ccagacgcca ctgacatacc gagcgggaac gagatcgacg
     3121 tgcatgtcgt agtcggggaa ctcgaccttc accgagcgat cgttcttgca caccctgtcg
     3181 tcgtactcgt ccgtcagcag gtcaacgaac ttatcgagca actcctgtgg atcggcatcc
     3241 tccggcaggt cctccaactc gcagaacacg tcaacgtcct tcacacgccg aattgacacc
     3301 tctcgcttgt acgagccgat aagaaccgtg tgaaggcccc agtcggacag ttcggcgtcg
     3361 cgctccaaat agtcacgtac ctcggtgtgt gcttcggcgg cgtgagcggc gtcgacacca
     3421 ggctcgatca tggagagtgc gtctttgaat tgtttgctga ggatcgccat cgggacccct
     3481 tcctcccgca cgctaggcgg gctccttgtc caacgaccaa cagtagggac aaccactgac
     3541 agtcctcccc cctttgggcc cacgggctgc ccgcctcgag ctggccaccc gatctgccga
     3601 gccgcggtct tgcctgcgcc gcgaacggcg cgttcgggct ggcctcgccc gacgccgcgg
     3661 gccgcgaggc ggcgctcgac atgctgcgcg acgtcgcggc gacggtgcgc gccttgaacg
     3721 agcaggcggg ccggcaggtg gtgttcgcac tggagatcca ctgcgccccc ggcttcgaca
     3781 agcgcacggc gatgcccgac gccgacgcct tccgccgctc gctcgacgtc gcggccacgc
     3841 tcgactg
//
LOCUS       DAOSPL010000250         3837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_23855_length_3837_cov_2.948176, whole genome shotgun sequence.
ACCESSION   DAOSPL010000250 DAOSPL010000000
VERSION     DAOSPL010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23855_length_3837_cov_2.948176"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PLQ54_05970"
     CDS             <1..326
                     /locus_tag="PLQ54_05970"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOS92839.1"
                     /translation="QAGAIILVAALLLAVLAPVIAGLIRMAISRKRELLADAEAAKIT
                     RYPKGLADALRKLEYDTEPLEAANKATAHMYIVNPLKDLGGFVNGLYSTHPPIAERVN
                     ALMAM"
     gene            337..1371
                     /locus_tag="PLQ54_05975"
     CDS             337..1371
                     /locus_tag="PLQ54_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92840.1"
                     /translation="MLLTCLILALGAGAWAAPDAALVRALAERILACRHEPTGVFLMA
                     PIAEGTPAGLSPYFAHFAAYGLLRAYEVVPDPEYLAAADAWVDWYREHMQADGTVTDY
                     RLEPDGRLTSAGDMDSTDSYAALYLWLIAERWRAKPEPAEWLRERELSCFRAYSAIML
                     TLQADGLTVAKPSYPIEYMMDNAEVIVGLWSGVSVFEALGNEGMATQCRRLGDRMEAE
                     LRTFYSPEHGYFAIYRDLQGRKGYQLTQWYPDVMAQVLALMRIGRAQEPDRSVFARVA
                     RDFLEKEFDLTRPLRHAWYGLAAQRVGDTDWADRIIGALGRLTPESVSNLDSVECNTV
                     LEAITGGTRP"
     gene            1532..1927
                     /locus_tag="PLQ54_05980"
     CDS             1532..1927
                     /locus_tag="PLQ54_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92841.1"
                     /translation="MLVLSALFAMGVGGLRSATWADDDDTDRRPVVRGGGFYRSGEGA
                     GVRDRATGRVYAPRGYYRSESWYRGGDRHRSRHHDDDVVIRYVHPYPRWRYSPGYWGH
                     WPYRSHWYGGLSLGYHGRHGHFGLTLGWP"
     gene            1982..>3837
                     /locus_tag="PLQ54_05985"
     CDS             1982..>3837
                     /locus_tag="PLQ54_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92842.1"
                     /translation="MGRGWCLAVVLGTAVPSWAQLVASIEPGGMAQYQVLISSGGTLR
                     EVIAPLEADAFSLAPSGRVAVLARADDLSIVHVNRGAVTRLHLEAPGPTMFVGWDATG
                     GRLWLTRGRALWLVDVAESRIRRMSGPGEAHEVRLSRDGSVAYYVDRDLASAAPGGTC
                     ALWLADSAQPEPAKLSGRLLVEAICGPETAGRLVFRATDPAAGASAFVVLDRERRRVL
                     PAAGSQSASKASLSPDGKWLALATAGGLMVSDLSGPPRVVAGAQDPWEVGDFLWVGPS
                     RLVWTQTSGGTVRALAVADVSPEGGPAPRLVLQRPAGRQAWRLCPSPTGGACLVSVPA
                     EPAGAETYLVGLDGLQEPIRLPLTAWPRDVAWAPDGSLVAFASGHEPAEEQMYVYDTE
                     AAGPGAFAGLGRARRPLWSADSTRLLFLACGPNERAPTAYLYDHRRGEPGRLGNGLPV
                     AGLEWEPDGRTARLCVHTRDTRGRALYALYAATPGRAPVLLSGPHRVAQWARTADGDL
                     VMVSVPRPVFAALRVHDLASGEVWSPSGELDVRHARWSPDGARICFTADVFEGRSALY
                     VAGARDVSARLVTERFLVSASSPPEWSPDGRRLALCSSGPNPTVWIYDAETG"
BASE COUNT          520 a         1275 c         1362 g          680 t
ORIGIN      
        1 gccaggcggg tgccatcatc ctggtggcgg ccctcttgct cgcggtcctg gctccggtca
       61 tcgccgggct catccggatg gcgatctcgc ggaagcggga gttgctggcc gacgccgagg
      121 cggcgaagat cactcgctat cccaagggcc tggccgatgc cctccgcaag ctcgagtacg
      181 acaccgaacc gctcgaggct gccaacaagg cgacagccca catgtacatc gtgaacccac
      241 tcaaggatct gggcgggttc gtgaacggcc tctactcgac gcatcccccc atcgccgagc
      301 gtgtcaacgc gctgatggcg atgtgaggtc cgccccatgc tgctcacctg cctcattctc
      361 gccctcggag ccggcgcttg ggctgctccc gacgccgctc tagtccgtgc gttggccgag
      421 cgcatccttg cctgccggca tgaacccact ggcgtgttcc tgatggcgcc gattgccgag
      481 ggcacgcccg cgggcctttc gccctacttc gcccacttcg cggcatacgg actgctgcgc
      541 gcctacgagg tggtgcccga ccccgagtat ctcgcggccg ctgatgcgtg ggtcgattgg
      601 tatcgcgagc atatgcaggc cgatggcacg gtcaccgact accgcctgga gcccgatggc
      661 cgcctcacgt cggccggcga catggactcc accgactcct acgcggccct ctatctgtgg
      721 ctcatcgccg agcggtggcg agcgaaaccc gagccggctg agtggcttcg cgagcgcgaa
      781 ctgagctgct tccgcgccta ctcggcgatc atgctgaccc tgcaggccga tggcctcacc
      841 gttgccaagc cctcgtaccc catcgagtac atgatggata acgccgaggt gatcgtcggc
      901 ctgtggagcg gcgtcagcgt gttcgaggcg ctaggcaatg agggcatggc cacccagtgc
      961 cggcgcttgg gcgaccggat ggaggccgag ttgcggacct tctacagccc cgagcacggc
     1021 tacttcgcca tctaccgcga cctgcagggg cgaaagggct accaactgac ccagtggtac
     1081 cccgacgtga tggcccaggt gctggcgctc atgcgcatcg gccgggcaca ggagcctgac
     1141 cggtcggtct tcgctcgcgt ggcgcgcgac ttcctcgaga aggagttcga cttgacccgt
     1201 cccttgaggc acgcgtggta tggcctcgct gcccagcgcg tgggtgacac cgattgggcg
     1261 gaccgcatca tcggtgccct cgggcggctc actcccgagt cagtctcgaa cctggattcg
     1321 gtcgagtgca acacggttct ggaggccatc accggcggca cccggccctg aggggatcac
     1381 gaggttctga ccgagtccga tgcacccttt cggcgcgtta ccgcgtcgta taatgtggga
     1441 gagagcctcg gtggagtggt ggcggggcag ttcatccctc aggcgaagtc gtgcctgaca
     1501 ggagaagggg gccccttggg tgagaatgct catgttggta ctgtctgctc tgtttgccat
     1561 gggggttggt ggcctgcgga gcgcgacctg ggccgacgat gacgacacgg accggaggcc
     1621 ggtggtgcgc ggcggcgggt tctaccgcag cggcgagggt gccggggtcc gcgaccgtgc
     1681 aaccggccga gtctatgccc cccgcgggta ctaccgcagc gagtcatggt accggggcgg
     1741 ggaccggcac cgcagccggc accacgatga cgatgtggtt atccgctacg ttcaccccta
     1801 cccccggtgg cgctactcgc cgggttactg ggggcactgg ccgtaccgaa gccactggta
     1861 cggcggtctg agcctcggct accacggacg ccacgggcac ttcggtctca cattagggtg
     1921 gccgtagggt ggcgtgtgag tccgggcgag ttggcgctcg cccaccgagg tggggccgct
     1981 gatggggcgc ggctggtgtc ttgccgttgt ccttgggaca gcggttccat cgtgggcgca
     2041 gctcgtggca tcgatcgagc ccggtgggat ggcgcagtac caggtgctta tctcgtcggg
     2101 agggactttg cgcgaggtga tcgcgccgct cgaggcagac gcgttctccc tggcgccttc
     2161 cggtcgcgtc gcggtgttgg cccgggcgga cgatctctct attgtccacg tgaatcgcgg
     2221 cgcggtgacg cgtcttcatc tcgaggctcc gggtcctacc atgtttgtgg ggtgggacgc
     2281 gaccggtgga cgcctttggc tcactcgggg ccgggcactc tggctggtcg acgtcgcgga
     2341 atcccggatc cgccgcatga gcggaccggg ggaggcccat gaggtcaggc tgagccgcga
     2401 tggctcagtc gcctactatg tggaccgcga cctggctagc gcggcccctg gcggcacatg
     2461 cgcgctgtgg ctcgccgatt ctgcacagcc cgagccggcc aagttgagtg ggcggcttct
     2521 ggttgaagcc atctgcggac cagaaaccgc cggccggctt gtgttccgcg ccacggaccc
     2581 tgcggccgga gcctctgcgt ttgtggtcct ggatcgtgag cgccggcggg tgctccccgc
     2641 ggcgggtagt cagtctgcgt ccaaagcctc gctatcgccc gatgggaagt ggctcgccct
     2701 cgccacggct ggcgggctaa tggtgagcga cctgtctggc ccgccccggg ttgtcgccgg
     2761 ggcccaggat ccgtgggagg tcggcgactt cctctgggtg gggccgtcgc ggctcgtttg
     2821 gacccagacc tcgggtggta cggttcgggc gctcgctgtg gccgatgtga gcccggaggg
     2881 cggtccggca ccccgtctcg tgctgcagcg gccggcgggc aggcaggcat ggcgcctgtg
     2941 cccgagccca acaggcggcg cgtgcctggt gtcagtcccg gccgagcccg ctggggccga
     3001 gacctacctc gtgggcctcg atgggctgca ggaacccatc cgcctgccgc tgacagcgtg
     3061 gccgcgcgat gtggcctggg cgccggacgg tagcctggtc gccttcgcga gtggccacga
     3121 acccgccgag gagcagatgt acgtctacga caccgaagcg gctggccccg gtgccttcgc
     3181 cggcctgggt cgggcgcgcc gtcccctgtg gtcggcggac agcacgcgac tgctcttcct
     3241 cgcctgtggc cccaacgagc gagcgccgac ggcatacctc tatgaccatc ggcgtggtga
     3301 gccgggacgc ctgggtaacg gcctcccggt cgccggcctc gaatgggagc ctgacggtcg
     3361 cacggcacgc ctgtgtgtgc atacccgcga tacgcgaggt cgggcactgt atgccctcta
     3421 cgcggccaca cccggccggg caccagtcct gctctcggga cctcaccgtg tggcgcagtg
     3481 ggctcgcact gccgacggcg acctggtgat ggtgtccgtc cctcgtcccg tctttgcggc
     3541 gctgagggtg cacgatctgg cgtcgggtga ggtgtggtct ccgagtggcg agctcgacgt
     3601 gaggcacgct cgatggtcgc cggacggagc gcgcatctgc ttcaccgccg atgtgttcga
     3661 ggggcgttcg gccctgtacg tggcgggcgc acgtgacgtc tcagccaggc tggtgacgga
     3721 gcggttcctc gtgagcgcct caagcccgcc cgagtggtcg cccgatggga ggcggctcgc
     3781 cctgtgttcc tccggcccca accccaccgt gtggatctac gacgccgaga cgggcag
//
LOCUS       DAOSPL010000251         3830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_23909_length_3830_cov_3.862517, whole genome shotgun sequence.
ACCESSION   DAOSPL010000251 DAOSPL010000000
VERSION     DAOSPL010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23909_length_3830_cov_3.862517"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PLQ54_05990"
     CDS             complement(<1..569)
                     /locus_tag="PLQ54_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92843.1"
                     /translation="MQAGHGTVTRAALAALLFLGPHCAASGTSGDEPTYLTILGQPEE
                     FAAEFPPTPKDASRQVGFAILLYTLKTPPEHMAWQVEQALDKAEQTGYPVLIHLDDWN
                     YPRASADPQVVEWTAFPQKGEYHGPLVRRRWINWGSWFTTEAPPNFESPRFRADVRQR
                     LKAIARPIAQRLRCWRVEGRAHLFAGLVVG"
     gene            692..2686
                     /locus_tag="PLQ54_05995"
     CDS             692..2686
                     /locus_tag="PLQ54_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92844.1"
                     /translation="MNRLVLALFLGVSWAAAGARSDEPGYRDIYPDTWVATDALGRTM
                     PAYSDVGPVKTDQRRVVGIFYITWHSDALGTLKSPYTADVTRILATDPKARLDAKHPL
                     WTEGSYHWGEPECGYFLSKDEYVIRKDMSMLADAGVDVLVMDVTNAVRYWDEWDVVFP
                     VMQKMKAEGNRVPQFVFWAFNGPCITVVQDLYDRIYKEGRYKDLWFYWDGKPLLLCNG
                     TPSVDANTGGIRHPNPHYDPAARTDPNHPHFGDPDYTEEFYRDYTKEVKEFFTLRTMW
                     WGYYEWAGKRFVGTEDNWSFGLDLGDPRVRALSPEELLSTHNGRREQAAVTPAQHPST
                     LTGKSWTREHGEPELNEYDLPVPSFVPWLGKTVANPEGYGIYFQDRWDEALQANPQFL
                     YINDWNEWTAGKYHPAEGQTTPFMRRQSTYFFVDQYNSEFNRCLQPMKGGYTDNYYMQ
                     MAQNIRRYKGVRPIPELRGLSSVRIDGDFADWDTATVEYRDTVGDVFHRDYPGYGGLH
                     YTNDSGRNDIITSKVAMGSTAVWFYAQTKDALTPHTGGNWMLLFIDADCDATTGWCGY
                     DFLVNRHIADAKTTTLMRYSRYAPREPWVRVATLDYRYSGNELELAIPRKLLGLIGNA
                     VTFDFHWCDNPSDLKDAISLCVNGDSAPNRRFNYRCVWRR"
     gene            complement(2882..>3830)
                     /locus_tag="PLQ54_06000"
     CDS             complement(2882..>3830)
                     /locus_tag="PLQ54_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOS92845.1"
                     /translation="EDLRLTPTIPEGPMGRVWMDFVSTHIPVEQWRIRDTLPEKAFQR
                     FALTRHGSVEAINSAYGLRLVSLEQLGIPFGPALLVTFANREGAFVRDNLIGNYAAVI
                     DYLVHRGLAVRNTVILVVLAVLIALTVNPLAAYALSRFRLRASEQIIVFCLATMAFPA
                     AVAAIPGFLLLRDLGLLNTFAALVLPSAANGMTIFLLKGFFDSLPKELYEAATMDGAP
                     EWTVFLRISLPLVKPILAVGMLNAFIAAYNGWEWAIIVAQDPKIWTIAVWTYEFSQTL
                     TGAPHLIMASFIVNSIPVLLVFLFCQKIILRGIILPQMK"
BASE COUNT          715 a         1256 c         1206 g          653 t
ORIGIN      
        1 ccgacgacca ggccggcaaa caggtgagcc cgcccctcga cccgccagca ccgcaaccgc
       61 tgtgcgatgg gccgggcgat ggccttcagc cgctgcctca cgtcggcgcg aaaccgcggg
      121 ctctcgaagt tggggggcgc ctcggtggtg aaccacgaac cccagttgat ccaccgccgc
      181 ctgacgaggg gcccgtggta ctcgcccttc tgggggaacg ctgtccactc cacgacctgg
      241 ggatcagcgg aggcgcgggg gtagttccag tcgtccaggt ggatcagcac cgggtaaccc
      301 gtctgctccg ccttgtcgag cgcctgctct acctgccatg ccatgtgctc gggcggcgtc
      361 ttcagcgtgt agagcaggat ggcgaacccg acctggcgcg acgcgtcctt cggggtgggc
      421 gggaactcgg cggcgaactc ctccggctga ccgaggatcg tgaggtacgt gggttcgtcc
      481 ccactcgttc cgctcgccgc acagtggggg ccgaggaaca gcagtgccgc cagcgctgcg
      541 cgcgtcacgg ttccgtgacc tgcctgcatg gctcgcacct ccgtagtccg caccgggccg
      601 atacaggacc cttgccaccc atggcagaac ctggcggggt cgagcgggtc ttcggtcccg
      661 catggttcat ccagagccat tggagcttgc catgaacagg ctcgtcctcg cactgtttct
      721 cggcgtgtcc tgggccgctg ctggagcgcg gtctgacgaa ccaggctatc gcgacatcta
      781 ccccgacacc tgggtcgcta ctgacgccct cggacggacc atgccggcgt actcggacgt
      841 cgggcccgtc aagacggacc agcgccgcgt cgtcggcatc ttctacatca catggcatag
      901 cgacgcgctg ggcacgctca agagccccta cacggctgat gtgacccgca tactcgccac
      961 cgaccccaag gcgcgcctcg acgccaagca cccactctgg accgagggtt cctaccactg
     1021 gggcgagccc gagtgcggct actttctgag caaagacgag tacgtcatcc gcaaggacat
     1081 gtccatgctc gccgacgctg gagttgatgt cctcgtgatg gacgtcacca atgcggttcg
     1141 ctactgggat gagtgggacg tcgtcttccc ggtgatgcag aagatgaagg cggaggggaa
     1201 ccgggttccc cagttcgtct tctgggcgtt caacggtccg tgcatcaccg tggtacagga
     1261 cctctacgac cgcatctaca aggagggcag gtacaaggac ctgtggttct actgggacgg
     1321 caagccactc ttgctctgca acggcacacc tagcgtcgac gcgaacaccg ggggtatccg
     1381 gcaccccaat ccccattacg accccgccgc caggacggac cccaatcatc ctcacttcgg
     1441 cgacccggat tacaccgagg agttctacag ggactatacc aaggaagtaa aggagttctt
     1501 taccctgcgc accatgtggt ggggctacta cgaatgggcc ggcaagcggt tcgtcgggac
     1561 cgaggacaac tggagcttcg gcctcgacct gggcgatccg cgggtccgcg ccctgagccc
     1621 ggaggagctc ctctcgacgc acaacggccg gagggagcag gcggccgtca ctcctgcgca
     1681 gcatccgtcg actctgacgg ggaaatcgtg gacgcgagag cacggtgaac cggaactcaa
     1741 cgagtatgat ctgcctgtgc cctccttcgt cccctggctc ggcaagacag tcgccaatcc
     1801 ggaggggtac ggcatctact ttcaggaccg gtgggatgag gctctccagg ccaacccgca
     1861 gtttctgtac atcaatgact ggaatgagtg gaccgcgggc aagtaccacc ctgccgaggg
     1921 acaaaccacg cccttcatgc gccggcagag cacctacttc ttcgtggatc agtacaactc
     1981 cgagttcaac cgctgcctcc agccgatgaa gggtggctat accgacaact actacatgca
     2041 gatggctcag aacatccggc ggtacaaggg cgtgcggccg attcctgagc tacgcggact
     2101 cagctcggtg cgcatcgatg gcgacttcgc cgactgggat acggccacgg tcgagtaccg
     2161 cgataccgtg ggcgacgtgt ttcatcggga ctaccccggc tatggtggtc tccactacac
     2221 caatgactcg ggccggaacg acatcatcac cagcaaggtc gccatgggct cgactgccgt
     2281 gtggttctac gctcagacga aagacgcact gacgccgcac accggcggca actggatgct
     2341 cctgttcatc gatgcagact gcgatgcgac caccggttgg tgcggctacg actttctggt
     2401 gaaccgccat atcgccgacg cgaagacaac aaccctcatg cgttactcac gctatgcccc
     2461 acgtgagccg tgggtccgtg tggccacgct ggactatcgc tattccggca acgagctcga
     2521 gctcgccatc ccgcgcaagc tgctcgggct gatcggcaat gccgtcacgt tcgacttcca
     2581 ctggtgcgac aacccgtcgg acctgaagga cgccatctcg ctgtgcgtca acggcgacag
     2641 cgcccccaac cgccgcttca actaccggtg tgtctggaga aggtaggggg aggccgcaga
     2701 ttgcccgggc cggatagggt cccaccatcc tccggatggt cctccctatg cggcctctcc
     2761 gaacaccttc cgcgcgccga ggaggggccg aatggaaggg gcccgcgaag cgggaagggc
     2821 gccatccggc ccgcgtttgg cgagcctcgc gtgcggggcc tgacgcccgg ggctcccgcc
     2881 ctcacttcat ctgcggcaag atgatccccc gcaggatgat cttctggcag aagaggaaca
     2941 ccagcagcac ggggatcgag ttcacgatga aggacgccat gatgagatgc ggcgctccgg
     3001 tgagcgtctg cgagaactca tacgtccaca cggcgatggt ccagatcttc gggtcctggg
     3061 ccacgatgat cgcccattcc cacccgttgt aggctgcaat gaaggcgttc aacatgccca
     3121 cggccaggat gggcttcacc aggggtagcg agatccgcag gaagaccgtc cactcggggg
     3181 cgccgtccat agtcgctgcc tcgtagagct ccttgggcag tgagtcgaag aagcccttga
     3241 gtaggaagat ggtcatgccg tttgccgcgc tgggcagcac cagggcggcg aaggtgttca
     3301 gcagccccag gtccctgagc agcagaaagc ccgggatcgc cgccacggcc gccgggaagg
     3361 ccatcgtcgc caggcagaac acgatgatct gctccgaagc cctcaggcgg aatcggctca
     3421 gcgcatatgc cgccagcggg ttgaccgtta gtgcgatgag aaccgcgagc acgacgagga
     3481 tcaccgtgtt ccgcaccgcg agcccgcggt ggaccaggta gtcgatcact gccgcgtagt
     3541 tcccaatcag gttgtctcgg acgaatgcgc cctctctgtt ggcgaacgtc acgagcagtg
     3601 cgggtccgaa tgggatgccg agttgctcca ggctcaccag cctgagcccg taggccgagt
     3661 tgatggcctc gacgctcccg tgccgggtca gggcgaacct ctgaaaggcc ttctcgggca
     3721 gcgtgtcgcg gatgcgccac tgctccacgg ggatatgcgt cgagacgaag tccatccaca
     3781 cccggcccat gggaccttcc gggatcgttg gcgtcaggcg aagatcctca
//
LOCUS       DAOSPL010000252         3817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_24026_length_3817_cov_3.143541, whole genome shotgun sequence.
ACCESSION   DAOSPL010000252 DAOSPL010000000
VERSION     DAOSPL010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24026_length_3817_cov_3.143541"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /gene="murJ"
                     /locus_tag="PLQ54_06005"
     CDS             <1..1387
                     /gene="murJ"
                     /locus_tag="PLQ54_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOS92846.1"
                     /translation="VPFFSEHIAKGEEDKAWRSFSAILTALLLGLVLLVGAMILLVPC
                     LVEVFVPSSEFAPEHQRAWVEMTRIMMPAQVFFVTGGLFMGVLNSYQHFWAPAVGPTV
                     YNLVVIGGIMLLGEHLGVKGPALTVPLGAFVGQWVIQIPVLVRKGARFRPNLDFTDPG
                     LRRVFLFMAPVTLGLSVIHINMLVARALSGQVSAEGPTYFENAYRIVMLPVGVFAMGA
                     AMAAYPTIASAAARTDAHGYRRALGEALRRVMFLTIPSMAILMVARRAAVDILFGHGK
                     FSPADVDATASALLFYAPGVIGMSAQQVLIRGFYALNDSRTPVYAGVAGVGVALGLGA
                     ALQPVMGVEGLCLALSVSAILVAAGMWCLLGRRPLARGALADSLPSMARCLLASVVTA
                     AAALAGQLALDGAWPVSRDLARQAAAALVPIATGLAGLAVGVTVFRVEEGVALGRVLA
                     RKLLPRRGGSG"
     gene            complement(1440..>3817)
                     /locus_tag="PLQ54_06010"
     CDS             complement(1440..>3817)
                     /locus_tag="PLQ54_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOS92847.1"
                     /translation="LLKICPKKPPPPDGKFSMSFRMAEIQDFLQFMADSTGYVFLVPP
                     ELTGKVTITTQVDVAADAAFHILDAWLSVKGFGMTRDDAAKLVIVETTKRAKTRGSEL
                     GTGLDPDAVPEGDQYVTHLALLKTIDAQAAVDLLKPLVDEQAGIVSASGDLNAIVISD
                     AASNVRRLVTVLSLLEREVDQTEVHTVAVVRLQNADAGSVANMLTQLFQTGMSSEQME
                     AAMRRGMPPPEAGGMEIPGSTLVGLRGQVRVAADARTNSVIVLASAEKIDMVKTILAK
                     LDDVTGREVIHKVFTIENADPADVVESLNELWEQPRGVVSSRSSPFSGFMSRMLMREG
                     KAFYGLKENVAIADTRTSKIVVTASPENMPVFEAMVKALDRPKPLQSLTRVFRLRNAD
                     AEAVATAINDALQGRQGGRGFLTMLFGGTANSGTPLDPLRQVTATAETITNQVIVTAL
                     PQAFPVVERMINDLDKRVPQVFVRVIIADVTLSNSETYGVEWNWFAPNVGGDAGTSGD
                     VSWTATDPLSSRDGLRWGVVSESIQAYLSAVKSKGNVRIISTPHIMTLDNQWGSIEIG
                     ERIPVLTGISESTSGRITQQIEYQEATIGLYVMPQVGESGFISLRLYQRIDDVERGRG
                     DANTPAGQPTFIQRRATSTVLVRDGQTVIIGGILQQAKRKVVDKVPILSEIPLLGNLF
                     RHKREEKGKTELMVFLTPYVVLDEDDAARHTREEADQMRVNVPGMDTSAKKETRWDKE
                     APGEAIPPSAPGAPAAPEAEPSALQPAPEPTSETVPAFRRPGDRLAWREAG"
BASE COUNT          513 a         1259 c         1294 g          751 t
ORIGIN      
        1 ggtgcccttc ttcagcgagc atatcgccaa gggcgaggag gacaaggcct ggcggtcctt
       61 ttcagcgatt ctcactgccc tgctgcttgg actggtcttg ctcgtcgggg ccatgatcct
      121 gctcgtgccc tgcctggtcg aggtgtttgt gccgtcctcc gagttcgcgc cggaacacca
      181 gcgagcgtgg gtcgagatga cccggattat gatgcccgct caggtcttct ttgtgaccgg
      241 cgggctgttc atgggggtgc tcaactccta ccaacacttc tgggccccgg cggttgggcc
      301 gacggtctac aacctggtgg tcatcggtgg catcatgctc ctcggtgagc acttgggggt
      361 gaaggggcca gccctcaccg tgcccctcgg cgcgttcgtc ggccagtggg tgatccagat
      421 cccggtgctg gttcgcaagg gcgcccggtt tcggcccaat ctggacttca ctgaccctgg
      481 tcttcggcgc gtgttcctgt tcatggctcc cgtaacgctc ggcctgtcgg tcattcacat
      541 caacatgctg gtggcccgcg cgctgtcggg ccaggtctcg gctgagggtc cgacctattt
      601 cgagaacgcg taccggatcg tgatgctgcc cgtgggcgta tttgccatgg gcgccgccat
      661 ggcggcctac ccgaccattg cgtcggccgc ggcccgcacg gacgcccacg ggtaccgccg
      721 cgccctgggc gaggcgctgc gacgcgtcat gtttcttacg attccctcca tggcgattct
      781 catggtcgcg cgccgcgcgg cggtggacat cttgttcggg catgggaagt tctcgccggc
      841 cgacgtggat gccacggcct cggcccttct gttctatgcg cccggcgtga tcgggatgtc
      901 ggcgcagcag gtgctcattc gcggcttcta tgccctgaac gactcgcgca cccccgtgta
      961 cgccggcgtt gcgggggtcg gtgtcgcgct gggccttgga gctgcgctgc agccggtcat
     1021 gggcgttgag ggcctgtgcc tggcgctgtc ggtctccgcg atcctggttg ccgcggggat
     1081 gtggtgcctg ctggggcgtc gtccgctggc ccgcggcgcg ttggccgact ctttgccctc
     1141 gatggcccgg tgcctgttgg cctcggtcgt cactgcggct gccgcgctgg cgggacagct
     1201 cgcgctcgat ggcgcgtggc cggtcagccg cgatctcgct cgccaggccg cggcggccct
     1261 ggtgcccatt gccaccggcc tggccggtct ggccgttggc gtgacggtgt tccgcgttga
     1321 agagggagtc gcgctgggcc gtgtgctggc gcggaagctc ctgccgcgtc gcggcggttc
     1381 gggctagcct ctcccgcggc ctcgggacct caacggacgg ccaggcggcc gcccgggttc
     1441 tagcccgcct cccgccacgc caaccggtcg ccgggccggc ggaaggccgg gaccgtctct
     1501 gaggtgggtt ccggggcggg ctgtagggcc gacggctccg cctccggcgc ggcgggcgcg
     1561 cccggtgccg acgggggtat ggcctcgccg ggagcctctt tgtcccagcg ggtttccttc
     1621 ttggccgacg tgtccatgcc cggcacgttc acccgcatct ggtccgcctc ttcccgtgtg
     1681 tgccgcgccg cgtcgtcctc gtcgagcacc acataggggg tgaggaagac catcagctcc
     1741 gtcttgccct tctcctcacg cttgtgccgg aacaggttgc cgaggagagg gatctcgctc
     1801 aggatcggta ctttgtcgac gaccttgcgc ttcgcctgct ggaggatgcc gccgatgatg
     1861 accgtctgcc catcgcgcac gagcacggtg ctcgtggcgc ggcgctggat gaaggtgggc
     1921 tgccccgcgg gtgtgttcgc atcgccccgg ccgcgctcaa catcgtcgat gcgctggtat
     1981 agccggagcg agatgaagcc gctttcgcct acctgcggca tgacgtagag gccgatggta
     2041 gcctcctggt actcgatttg ctgggtgatc cggccactgg tgctctcact gatcccggtg
     2101 aggacgggaa tccgctcccc gatctcgatg gagccccact ggttgtcgag cgtcatgatg
     2161 tgcggtgttg agatgatccg gacgttgccc ttgcttttca ccgccgagag gtaagcctgg
     2221 atggactcgg acacgacgcc ccagcgcagg ccatcacggc tcgagagggg atcggtggcc
     2281 gtccagctca catcgcccga ggttcccgca tcgcccccga cgttgggcgc gaaccagttc
     2341 cactcgaccc cgtacgtctc tgagttgctg agcgtgacgt cggcgatgat gactcgcacg
     2401 aacacctggg gcacgcgctt gtcgaggtcg ttgatcatgc gctcaacgac cgggaaggcc
     2461 tggggcaatg cggtcacgat cacctggttc gtgatggtct cggccgtggc agtaacctga
     2521 cgcagcggat ccagcggcgt gcctgagttc gccgtcccgc caaacagcat ggtcaggaac
     2581 ccgcgaccgc cctgccggcc ctggagtgcg tcgttgatgg ccgtggccac ggcctcggcg
     2641 tcggcattcc gcagccggaa cacgcgcgtg aggctctgga ggggcttggg gcggtccagg
     2701 gccttgacca tggcctcgaa gaccggcatg ttctcgggcg aagctgtcac cacgatcttg
     2761 ctggttcgcg tgtctgcaat cgccacgttc tccttgagtc cgtagaacgc cttgccctca
     2821 cgcatgagca tccgcgacat gaagcccgag aagggcgaac tgcggctgct caccacgccg
     2881 cggggctgct cccacagctc attgagcgac tcgacaacat cggcagggtc ggcattctcg
     2941 atcgtgaaca ccttgtgaat gacctcgcgg cccgtcacat catcgagctt ggccaggata
     3001 gtcttcacca tgtcgatctt ctcggccgag gcgaggacga taaccgagtt ggtgcgtgcg
     3061 tcggcggcca cccgaacctg gccccgaagg cccacgagag tgctgcccgg gatctccatg
     3121 ccgccggcct cgggcggagg catcccccgc ctcatggccg cctccatctg ctcggaggac
     3181 atccccgtct ggaacagctg ggtcagcatg ttggcgacgg agccggcatc ggcgttctga
     3241 agccgcacca ccgccaccgt gtgcacctcg gtttgatcga cttccctctc gaggagcgag
     3301 agcacggtga ccagtcgtcg tacgttgctg gccgcgtcgc tgatgacgat ggcgttcaga
     3361 tcgccggagg cgctcacgat gcccgcctgc tcgtccacca gcggtttcag gaggtcgacg
     3421 gccgcctggg catcgatggt cttcaggagc gccaggtgtg tcacgtactg gtcgccctcg
     3481 ggcacggcat cgggatccag cccggtgccc agctccgagc cgcgcgtctt ggcccgcttg
     3541 gtcgtctcga cgatcacgag cttcgcggcg tcatcccggg tcatcccgaa gcccttgacg
     3601 gacagccagg cgtccagaat gtggaatgcg gcatcggcgg ccacatccac ctgggtggtg
     3661 atggtgacct tgccggtcag ctcggggggc acgaggaaca catagcccgt actgtcggcc
     3721 atgaactgca ggaagtcctg gatctcggcc atgcggaagc tcatggagaa cttcccgtcg
     3781 ggcggtggcg gcttcttggg acagatcttg agcagtt
//
LOCUS       DAOSPL010000253         3816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_24033_length_3816_cov_3.909864, whole genome shotgun sequence.
ACCESSION   DAOSPL010000253 DAOSPL010000000
VERSION     DAOSPL010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24033_length_3816_cov_3.909864"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLQ54_06015"
     CDS             complement(<1..661)
                     /locus_tag="PLQ54_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOS92848.1"
                     /translation="MPAKPRYLSPYDSVQAWIANETSELTFAGTTLEDYAQWRAAFDA
                     RLRQYLGRAPLPVPLDSEVLGVEDCGDHLREKVVFDSEKYASVIAYVLVPKGLAPGQK
                     LPAVLAAHGHGRGKLDVCGVTTTDDEAEFVRALNYDYARQFVRRGYVVIAPDWRGFGE
                     RSSPAEWARQGRDACNVNYMACGYIGYHLLALQIWDGMRCIDYLETRPEVDATRIGVA
                     GL"
     gene            complement(782..>3816)
                     /locus_tag="PLQ54_06020"
     CDS             complement(782..>3816)
                     /locus_tag="PLQ54_06020"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOS92849.1"
                     /translation="VRVRSARETMDAIAAKASIGDDEARLLARWLVAHQKLGDAEKLL
                     ARDLPRGVKADIACVLAKATPPGPKRARFLAAMVANGATSLNDGRKRLAEVSEGLPSD
                     ALWSAVLSAIQDPATDAQSVDSLVAYAGDLTLATSSTQTLADRRRSADSLRKLVDAAQ
                     GKTAASSPARAALDKVAAALAAVRAELDAAQAQLGSCTIAIGGRVVRAESHSIVIPAK
                     PTPQEALAARDLQAHLEKITGSAPPVVTEGKAKTPFRIVVGKCQAVRNLGVAVDYAAL
                     GADGIRIKTSGPNLVLTGNKRGVLYAVYRFLEDQLGCRWFTPDCTTWPREGRINVGRL
                     DITYVPPLEYRATDYPDSRPPEFAVRNQYNGTQIEASEEWGGKISYRGFVHTFNYLVP
                     PEVYFDAHPEYFSEINGKRTRDYTQLCLTNPDVLRIAIATVRKWIQESPEATIISVSQ
                     NDWHNYCTCPKCSALAEEEGSQSGPLLHFVNAIADDIAQDYPHIIIDTLAYQYTRKPP
                     KHVKPRPNVAIRLCSIECEFNRPLETSPFNKSFVDDIRGWNQICDRLHIWDYVINYAH
                     CVQPFPNLRVLQPNIRFFIENGVTGIYEEANYFSRGGEMAELRTYLMAKTLWDPGYDI
                     EKGIDEFLAAYYGPAAPVIRRYIDDIHDLAVSDDDFHMPIYVGPKSPFQTDEALTRYE
                     GWFDEAEALVKDDAIRLHRVEVARLPIIYTRIAQASDRAYKLGPDALEADANDRITAL
                     IDRFERVARAEGLTAVSEGGVGSNLDRWLAHVRSRPQREPVARLQGGGLEVVVIPGMG
                     GRILSLKRLADGREFMDVNSTAAGIDPTVGGYEEFSESNYRSPGWNERYEVADRSDRA
                     ITLRANLANGLAVERRYALDSVQPVLRVSTTLTNASPEPREACIRVHPCFRMDDAGQA
                     VVRLGPPGAKEVGLASGQSQETELWLRGNDRPAGEWALIDQAAGVALVSRFDPAQVEI
                     CYLNWSGPDRRANLELWSPSARLEPGESITLAHAYEIA"
BASE COUNT          626 a         1253 c         1304 g          633 t
ORIGIN      
        1 acaggccagc gacgccgatc cgcgtagcgt cgacctcggg ccgggtctcg aggtagtcga
       61 tgcagcgcat gccgtcccag atctgaagtg cgagcaggtg gtaaccgatg tagccgcagg
      121 ccatgtagtt gacattgcac gcgtcacggc cctgccgcgc ccattcggcc gggctgctcc
      181 gctcgccgaa cccacgccag tcgggggcga tgacaacata tccccggcga acgaactgac
      241 gggcgtagtc gtagttcagg gcccgcacga actccgcctc gtcgtccgtc gtcgtcacgc
      301 cacagacatc gagcttaccg cgaccatgcc catgggcggc cagaacagcc ggcagcttct
      361 gccccggagc cagacccttg ggcaccagga cgtaggcgat gaccgacgcg tacttctcgc
      421 tgtcgaatac caccttctcg cgcagatggt cgccgcagtc ctcgacgccg agcacctccg
      481 aatcgagggg caccggcaag ggcgcgcggc ccagatactg ccggagccgg gcatcgaatg
      541 cggctcgcca ctgggcgtag tcctcaagag tggtcccagc gaaggtcagc tccgacgtct
      601 cgttggcaat ccaggcctgg acgctgtcat agggcgagag gtaacgcggc ttggcgggca
      661 tcgtgggtct cctattcggc ggcccaggcg accgcatcgg gaatccgggc cggacggggc
      721 ccacccggct tggcctggtt cccccatgcg gtgcgtctga accgcccgcg ggcagtccgg
      781 cctaggcgat ctcgtacgcg tgggcgagag tgatgctctc ccccggctcg aggcgggccg
      841 atggcgacca gagctccagg ttggcccggc ggtccgggcc gctccagttg aggtagcaga
      901 tctcgacctg agccggatcg aaccgcgaga ccaacgccac accggcagcc tgatcgatca
      961 gtgcccactc accggcgggg cggtcattcc cgcgtagcca gagctcggtc tcctggctct
     1021 ggccggaagc caagccaacc tccttggccc ctggcggccc cagacgcacc accgcctgcc
     1081 ccgcatcatc catccggaag caggggtgga cgcgaataca ggcctcccgc ggctccgggc
     1141 tggcattcgt cagcgtcgtc gaaacacgga gcacgggctg gacggagtcc agagcatacc
     1201 tgcgttccac ggcaagcccg ttcgccaggt tggctcgcag cgtgatagcg cggtccgacc
     1261 ggtcggcgac ctcgtagcgc tcgttccagc caggcgagcg atagttcgac tcactgaact
     1321 cctcataccc cccaaccgtg gggtcaatgc cagcggccgt ggagttgaca tccatgaact
     1381 cgcgcccgtc ggccagacgc ttgaggctga ggattcgtcc gcccatgccc ggtatgacca
     1441 cgacctcgag gcccccgccc tgaaggcggg ccacaggctc gcgctggggt cgggagcgca
     1501 cgtgggcgag ccaacggtcg aggttgctgc cgacaccgcc ctccgacacg gccgtaaggc
     1561 cctcggcccg ggcaacacgc tcgaagcggt caatgagcgc ggtgattcgg tcgttggcgt
     1621 cggcctcgag cgcgtcgggg ccgagcttgt aagcccggtc cgatgcctgc gcgatacgcg
     1681 tgtagataat cggcagccgc gcgacctcca cacggtggag acggatggca tcgtccttca
     1741 ccagggcctc ggcctcatcg aaccagccct cataccgcgt cagggcctca tcggtctgga
     1801 acggcgactt cggacccacg tagatcggca tgtggaagtc gtcgtcggag acggccaggt
     1861 cgtggatgtc gtcaatgtac cggcggatga caggagcagc cggcccgtag tacgcggcga
     1921 ggaactcatc gatgcccttc tcgatgtcgt agccgggatc ccacagggtc ttggccatca
     1981 ggtacgtccg gagctccgcc atctccccgc ctcgggagaa gtagttggcc tcctcgtaga
     2041 tccccgtcac gccgttctcg atgaaaaacc ggatgttcgg ttgcagcacg cggaggttcg
     2101 ggaagggctg cacgcagtgg gcgtagttga tgacgtagtc ccagatgtgc agacgatcgc
     2161 agatctgatt ccacccgcgg atgtcgtcga cgaagctctt gttgaagggc gatgtctcga
     2221 gaggccggtt gaactcacac tcgatgctgc acaaccggat ggccacgttg gggcggggct
     2281 tcacatgctt gggcggcttc cgcgtgtact ggtaggccaa ggtgtcgatg atgatgtgcg
     2341 ggtagtcctg ggcgatgtcg tcggcaatgg cattcacgaa gtggagcagc gggccgctct
     2401 ggcttccttc ctcctcggca agcgcgctgc acttggggca ggtgcagtag ttgtgccagt
     2461 cgttctgcga gaccgagata atggtggctt cgggcgactc ctggatccac ttccgaacgg
     2521 tcgcaatggc aatccgaagt acatccgggt tcgtcagaca gagctgggtg tagtcgcggg
     2581 tacgcttccc gttgatctcg gagaagtact cggggtgggc gtcgaagtac acctcggggg
     2641 gcaccaggta gttgaacgtg tgcacgaagc cccggtagct gatcttgccg ccccactcct
     2701 cgctggcctc gatctgcgtc ccgttgtact ggttgcggac ggcgaactcg ggcgggcgcg
     2761 agtccgggta gtcggtggca cggtactcga gcggcgggac gtaggtgatg tcgagccggc
     2821 cgacattgat gcgcccctcc cgtggccacg tggtgcagtc cggcgtgaac cagcggcagc
     2881 cgagctggtc ctcgaggaag cggtagacgg catagagcac gccgcgcttg ttgccggtca
     2941 gcaccaggtt gggaccactc gtcttgatgc ggatgccgtc agcccccagg gcggcatagt
     3001 ccaccgcgac gccgaggttc ctcacggcct ggcacttgcc gaccacgatg cggaacggcg
     3061 tcttggcctt gccctcggtg accacgggcg gggcgctgcc ggtgatcttc tcaagatgcg
     3121 cctgcaaatc acgcgccgcc agcgcctctt ggggcgtggg cttggcgggg atgacgatcg
     3181 agtgcgactc ggcccggacc acccgcccgc cgatggcgat ggtgcagctc ccgagctgtg
     3241 cctgagcggc gtcgagctcg gcacgaaccg cggccagcgc cgcggcgacc ttgtcgagag
     3301 cagcgcgggc gggggagctg gccgcggtct tgccctgcgc ggcgtcgacg agcttcctca
     3361 ggctgtcagc cgagcgccgg cggtccgcga gggtctgcgt cgacgatgta gcgagagtca
     3421 gatcgccagc gtaggcgacc agggagtcca ccgactgcgc gtcggttgcg ggatcctgga
     3481 tggcgctgag cacggcggac cacagggcgt cggaggggag gccctcgctc acctcggcga
     3541 ggcgcttccg gccatcgttg agcgacgtgg caccgttggc caccatcgcc gccaggaagc
     3601 gggctcgctt cgggccgggc ggagtcgcct tggccaagac acacgcgatg tcggccttca
     3661 ccccgcgcgg taggtcgcgg gcgaggagct tctcagcgtc accgagcttc tgatgcgcca
     3721 ccagccaacg ggcgagcagg cgggcctcat cgtcgccgat ggacgccttg gccgcgatgg
     3781 cgtccatcgt ctcccgagcc gaacggaccc gcacaa
//
LOCUS       DAOSPL010000254         3814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_24045_length_3814_cov_4.064113, whole genome shotgun sequence.
ACCESSION   DAOSPL010000254 DAOSPL010000000
VERSION     DAOSPL010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24045_length_3814_cov_4.064113"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /gene="gltX"
                     /locus_tag="PLQ54_06025"
     CDS             <1..1138
                     /gene="gltX"
                     /locus_tag="PLQ54_06025"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOS92850.1"
                     /translation="AAGRVPRYDGRCRGLDPDEAARRAEAGEPCVLRFRMPDMERLKV
                     PDLIRGEVVFEAREFDDFIIVKSDGFPVYNFANVVDDAGMKMTHVIRGEDLLPSTPRQ
                     LVLYDALECPPPQFAHAPMLLTPERRKLSKRDLPEGALAYRDEGYLPVAVVNFLSLLG
                     WSPGDDREVFTLDEIVQAFSIDRISPAPAVFDKQKMEWLNAQHLKRTPGSELAPLVRE
                     RMVAAGMLGPGEPSDEEAGYLVAVVDLMKERARTLNQLVEHSRFFFSDEYAFDEVGVR
                     KRLLKPEGMADILVGLAGSLDAVAPWTAEAIETSFRASADAQGVAHGTAIHGARVAVS
                     GQTVGPGLFDLLAVLGRERTTARLRAAAEKLRAGAFLSGSGTTA"
     gene            1185..3422
                     /locus_tag="PLQ54_06030"
     CDS             1185..3422
                     /locus_tag="PLQ54_06030"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOS92851.1"
                     /translation="MAALARGLIRTWLVRLVVAAGAAGAWAQNDPTEGASPGGGLSLA
                     LILLLAVVGIGIVALMAFLAFYSNTKRREQAGRSAAALAAKQIEARHQRALGRLAQER
                     ELLHRELEKAQAQARKADRQVEGRPDDPVDRGEVRVRAWIDSVVDLLWHPDIPGAKLT
                     RAQALAIDRDVSRWVPYDAALSGRRSLSQDETGVLPPQSRRLADVLRETTRAVLIGGP
                     GSGKTMALRTIALAYARDRTKEVFGFRESRLAAYIRLGSLAPEDLTRPLPQVLADQTF
                     PEGVGAAAFVETHLKAGSFILLFDDLHLVPSHELREQALRWIEAAETAYRKNVFIIAT
                     RPGDYGGHLRLPVQFREYSLVPLGPTEVEHVTASVATALALTHHPDGASAEECAGEVQ
                     RICATITNALADWGISPEATSPLDIVAAALAPVAGLETPRSEAERLQRLVTALCGGHA
                     TPEAPRPVTDAGTTVRLLAMLAYRLHTTGRSDVSSAKVLGFLEEALVGTPIDPSEARQ
                     VLRFAIDGATVLRADAGGRVRFVIPSVQEILTAVHARYATDPMALVSRLHEPFWHEVI
                     VHVCCLLDSRDMIQRLAYVPEEEFAANWPVVWRCACHVLGEGVRSHAFARRALEALSQ
                     GATSSDRIIRAAAVRALGDLRIAGCSEVLSRASEDRHSVVRLCAARAMGVMPDTAGAQ
                     SLVALLTDRDELPEVRCAAASSLAMLGYVDAVPHLASMADDPNPRVSECARLAVASLT
                     PAEEP"
     gene            3575..>3814
                     /locus_tag="PLQ54_06035"
     CDS             3575..>3814
                     /locus_tag="PLQ54_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92852.1"
                     /translation="MEALDVSTAVPNDRNPSRLRFRAVIVGLLLIPLNAYWLAIVELV
                     RNTQTPTSVSLFFNVIFTVVLVSLANAPLRRWVPAL"
BASE COUNT          604 a         1277 c         1297 g          636 t
ORIGIN      
        1 ggcagcggga cgggtacccc gctacgatgg ccggtgccgc ggtctggacc ccgatgaggc
       61 cgcgcggcgc gccgaggccg gagaaccgtg cgtcctccgg ttccggatgc ccgacatgga
      121 acgcctcaag gtccccgatc tcatccgggg cgaggtcgtg ttcgaggccc gcgagttcga
      181 tgacttcatc atcgtcaagt ccgatggctt ccccgtctac aacttcgcca acgtcgtgga
      241 tgacgcgggg atgaagatga ctcacgtcat tcgcggcgag gaccttctcc ccagcacgcc
      301 gcgccaactt gtgctctatg acgccctcga atgcccaccg ccgcagttcg cccacgcccc
      361 catgttgttg actcccgaac ggcggaagct ctccaaacga gacctgcccg aaggcgcgct
      421 cgcataccgc gatgagggct acctaccggt ggcggtcgtc aacttcctat cgctactcgg
      481 atggtctccc ggagacgacc gtgaagtgtt cacgctggac gagatcgtcc aggcattcag
      541 catcgaccgc atctcgccgg ctcctgcggt gttcgacaag cagaagatgg agtggctcaa
      601 cgctcagcat ctgaaacgga cgccaggatc ggagctggct cccctggtca gggagcggat
      661 ggtcgcggcc gggatgctcg gtccgggcga gccgagcgac gaggaggccg ggtaccttgt
      721 ggcagtcgtc gacctgatga aggaacgcgc gcgcaccctg aaccagctcg tggagcactc
      781 gcgtttcttc ttcagcgacg aatacgcgtt cgatgaggtc ggtgtgcgca agcgccttct
      841 gaagccagag ggcatggcgg acatattggt ggggctggcc ggctcgctcg acgctgtggc
      901 accgtggacg gccgaggcca tcgagacgtc ctttcgggcc agtgcggacg cccagggagt
      961 tgcgcacggc accgccatcc acggagcaag ggtcgccgtg agtggacaaa ccgtggggcc
     1021 aggcctcttc gacttgctgg cggtgctggg acgcgagcgg acaactgcac gactgcgtgc
     1081 ggcggccgag aagctgcgtg ccggcgcatt cctgtcgggc tccggcacca cggcgtgaac
     1141 cgttggccac cccctcggcg acacgccgga gcgggaggat gtgcgtggcg gcgctggcgc
     1201 ggggtctgat acgcacctgg ctcgttcggc tggtagtggc ggcgggcgcg gccggtgcgt
     1261 gggcgcagaa cgaccccacg gaaggcgcat cgcccggcgg cggtctcagt ctggccctca
     1321 tcctactcct cgctgtcgtc ggcatcggca tcgtggctct catggcgttt ctcgcgttct
     1381 actcgaacac gaagcgccgg gagcaggccg ggcgatcagc ggccgccttg gcggccaagc
     1441 agatagaagc ccggcaccag agagctctgg gccgcctcgc ccaagagcgg gagctgcttc
     1501 accgggagct cgagaaagct caggcacagg cacgcaaggc cgaccggcag gtcgagggac
     1561 ggcccgatga cccggttgac cggggcgagg tccgcgtgcg cgcatggatc gactcggttg
     1621 tcgatctctt gtggcatcct gacatccccg gggcgaagct cacgcgagct caggcgctgg
     1681 ctatcgaccg cgatgtgagc cggtgggtcc catacgatgc cgcgctatcg ggacgacgtt
     1741 cgctgtcgca ggatgagacc ggtgtcctgc ccccgcaatc gagacgactt gctgatgtgc
     1801 tccgcgagac gacccgtgcg gtgctcatcg gtgggccggg atccggcaag accatggcgc
     1861 tgcgcaccat agccctcgcg tacgctcggg accgcaccaa agaggtcttc gggttccgcg
     1921 agtcgcggct cgcggcatat atccggctcg gatcactggc ccccgaggac ctgacacgtc
     1981 cactcccaca ggtactggcg gaccaaacct tccccgaagg cgtgggggct gccgccttcg
     2041 tggagacgca cctgaaggcc ggtagcttca tcctcctgtt cgatgaccta cacctcgtcc
     2101 ccagccatga gctccgcgag caggccctcc gatggatcga ggcagccgag actgcgtacc
     2161 ggaagaacgt gttcatcatc gccacacggc cgggcgacta tggcgggcac cttcgactcc
     2221 ccgtccagtt tcgggagtac tcactcgtgc ccctgggccc caccgaggtc gagcatgtga
     2281 cggcaagcgt cgccacggca ctggcgctca cgcatcatcc ggacggagcg tcggctgagg
     2341 aatgtgcggg cgaagtccag cggatctgcg ccaccattac caacgccctg gcagactggg
     2401 ggatctcgcc ggaggcgacc tccccgctgg acatcgtggc tgccgcgctg gctcccgtgg
     2461 cgggactcga gacgccccga agtgaagcgg agcgcctcca gcgcttggtc accgcgctct
     2521 gcggggggca tgcgacgccc gaggcaccgc ggccagtgac cgatgccggt acgacggtgc
     2581 ggctcctcgc gatgctggcg taccgcttgc acacgaccgg gcgatcggat gtgagttccg
     2641 ccaaggtcct cgggttcctc gaggaggccc ttgtcgggac tcccatcgac cccagtgagg
     2701 cacgacaggt cctgcgattc gccatcgacg gggccaccgt gctccgggca gacgccggag
     2761 gacgcgtgcg attcgtgatc ccctccgtcc aggagatcct gacggcggtt cacgccagat
     2821 acgcgaccga cccgatggct ctggtgtcgc ggctgcatga gccgttctgg cacgaggtga
     2881 tcgtccatgt gtgctgcttg ctcgattcga gagacatgat ccagcggctt gcctacgtcc
     2941 ccgaggagga gtttgcggcc aactggccag tggtgtggcg atgcgcgtgc catgtcctcg
     3001 gtgagggcgt ccgttcacat gccttcgccc ggcgtgcgct tgaggcgctg tcgcaggggg
     3061 cgaccagttc cgatcgcatc atccgcgccg ccgcggtacg cgcgctggga gacctgcgca
     3121 tcgcggggtg cagcgaggta ctctcgcgag catccgagga tcgccacagc gtggtacgac
     3181 tgtgcgccgc tcgggccatg ggcgtcatgc cagacacggc gggcgcgcag tcgctcgtgg
     3241 cgctgctcac cgatcgggat gaactgccgg aggtccgatg tgccgcggcg tcgtcactcg
     3301 ccatgctggg ctatgtggac gccgtacccc acctcgcgtc aatggcggac gatccgaacc
     3361 cgagggtctc cgagtgtgca cggctcgcgg tggctagcct gaccccggcc gaagaaccct
     3421 aacgcggccc cggcgggagg gtggactacc gaggggtccg aacaccccgg cgcccgtgcg
     3481 ttccggccgg cgcgaccgac aacccaaacc ggagccgaga ggggggtgtc ccgacggccc
     3541 gctgcgtggc ggtgcctacc gggacccgtt gccgttggag gccctggacg tcagcactgc
     3601 cgtgcccaac gaccgcaacc cgtcgcgtct caggttccgt gcagtgatcg tgggcttgct
     3661 gctcatccct ctcaacgcgt actggctggc gatcgtcgag ctggttcgga acacgcagac
     3721 accgacctcg gtctccctct tcttcaatgt catcttcacc gtcgtcctgg tgtctctggc
     3781 caacgctcca ctccggcgat gggtgccggc gttg
//
LOCUS       DAOSPL010000255         3805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_24128_length_3805_cov_3.917867, whole genome shotgun sequence.
ACCESSION   DAOSPL010000255 DAOSPL010000000
VERSION     DAOSPL010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24128_length_3805_cov_3.917867"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PLQ54_06040"
     CDS             complement(<1..210)
                     /locus_tag="PLQ54_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92853.1"
                     /translation="MLRQVEPADVHIPSGPRDAARAGQFRRIQGTTAYVAGLGARLAK
                     PHPFEPIIGWSEGHWNGHAWRVAFGN"
     gene            complement(286..>3805)
                     /locus_tag="PLQ54_06045"
     CDS             complement(286..>3805)
                     /locus_tag="PLQ54_06045"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOS92854.1"
                     /translation="AIGLSAVPPNLAAEEPPREVAEWPDGLGNHRAVIQVSEPAEAVR
                     ARIPWRRRDPDPEAKRVIVVEAASGQPVANTVAASVTREAGDLVFEARTAGEYYAYYM
                     PFETTGWEYSPTCTYLKPEDTADPTWRQRVAGGVGLLPEARVTAIQARTDFHRFDPME
                     VIATEAETQALLAAHPGKPYLVFPEDRLHPIRMASDLPLRWIEAGPSTSFRCEAKRGE
                     LLAFQIGLYAARQAIEGLTAEPAPLAGGGGAFLPARGLTCINTSGSDWLGRPIAKTVS
                     VDQGRVQALWFVFAVPRDARPGAYEGPLTLKPANAPSTQVTLRLTVLDEVSPDGGVGN
                     VSNHSRLAWLNSRIGLDDEVVAPYTPVEVSGNTLRVLGRSVTLDHTGLPAQIHCPGGD
                     LLARPMAFSVERRGVEGRWTGGLPRLVNQAPGKVAYHAASAGSGLALSCDVDAECDGY
                     LNYRLVLRADRDTEVSDVRLDIPFRRDQATYMMGLKRKGGFRPDRWEWKWETAPHNSF
                     WIGTAQAGLHCRLKTSDQSWDLGNSGFPPSWHNEGKGGASVFEEGDTVVARVYTGRRS
                     LRAGEELEFRFGLLITPVKPLDPAHWNQRYYHMYAPPEEAVANGATVINIHHGNDLNQ
                     HINYPFLQTRELSDYVTRAHEKGLKVKIYYTVRELSDFCAEIWALRSLGDEIFVDGPG
                     GQKDSWLQEHLIDHYIPAWHQPLGPARIDAAIATVGLSRWHNYYLEGLAWLIRNVGID
                     GLYLDGIGYDREIMKRARKAMDRARPGCLIDFHSGNDYVYMDRKVSPACLYAEHFPYV
                     NSLWFGEMYDYNEPPDYWLVEVSGIPFGLYGEMLQDNGNPWRGMVYGMTARYYSGADP
                     KHIWKLWDEFGIQDSETLGYWDPRCPVRTGRDDIPATAYRKKGRTLIALASWAQERAK
                     VRLAIDWSALGLKAERACLYAPAIEGFQREAVFRPGDEIPVVPGRGWLLVVDESEREA
                     PAREDATAGLRVLLEDDFDGTALSPEWKTKLSMRPGTALSVADGTMQIHAMANVCALT
                     ERPLPPGATLVQCRVFSGTDAGASWGPGLGLVWPNGWWRVNVRAEGRFGADNGQAQTF
                     GGWSAPRRWYDLRIRLAGDTWVAEVGEDGEYWEIIGTRPAAELDGLPTAVRIGKMAGD
                     GSNTDYEVLGPEGDCQIDTLRVWGRQ"
BASE COUNT          577 a         1304 c         1278 g          646 t
ORIGIN      
        1 gttcccgaac gctaccctcc acgcgtggcc gttccagtgc ccctccgacc agccgatgat
       61 cggctcgaac ggatgcggct tcgcgagcct cgcgcccagg ccggcgacgt acgccgtcgt
      121 gccctggatc cggcggaact ggcccgctcg ggccgcatcg cgcggtcccg aggggatgtg
      181 cacgtctgcc ggctcgacct gccgcagcac acgtcgcagt gcgccgccgc ccggctcgga
      241 gactcgcgat gacgatgacg cacctcactg tcgccgggat ggcgcttact ggcgccccca
      301 gacccgcagc gtgtcaatct ggcagtcacc ttcgggcccc aagacctcgt agtccgtatt
      361 gctgccgtcg cccgccatct tgccgatgcg gaccgcggtg ggcaaaccat cgagttcggc
      421 cgccggccgc gtgccgatga tctcccagta ctcgccgtcc tcgcccacct cggccaccca
      481 cgtgtcgccc gccaggcgaa tacgtaggtc ataccaccgc cggggcgcgg accatccgcc
      541 gaaggtctgc gcctgaccgt tgtcggcccc gaaccggccc tcggcgcgca cgttcacgcg
      601 ccaccatccg ttcggccaca ccagtccgag cccaggcccc cacgatgcgc cggcatcggt
      661 gcccgagaag acccggcact gcacgagggt cgctccaggg ggcagtggcc gctccgtcag
      721 ggcgcagacg ttcgccatgg cgtggatctg catggttccg tcagcgacgg agagtgcggt
      781 tcccggacgc atggagagtt ttgtcttcca ctcggggctg agtgccgtgc catcgaagtc
      841 gtcctcgaga aggacccgca gccccgcagt ggcgtcctcc cgtgccggcg cctcccgctc
      901 ggattcgtcg acgacgagca gccagccgcg acctggtacc acggggatct catcaccggg
      961 cctgaacacc gcttctcgct ggaagccctc gatcgccggc gcatagaggc aggctcgctc
     1021 cgccttgagc ccgagggcgg accagtcgat ggcgagccta actttggcac gctcctgggc
     1081 ccaactcgcc agcgcgatga gtgtgcggcc cttcttccga taggcggtcg cggggatgtc
     1141 gtcccgcccg gtgcgaacgg ggcatcgcgg gtcccagtag cccaacgttt cgctgtcctg
     1201 gatgccgaac tcgtcccaga gcttccagat gtgcttcggg tcggcccccg agtagtaccg
     1261 cgccgtcatg ccgtagacca ttccgcgcca cggattgccg ttgtcctgga gcatctcgcc
     1321 atagaggccg aaggggatgc cggacacctc gacgagccag tagtcgggcg gttcgttgta
     1381 gtcgtacatc tcgccgaacc acaggctgtt cacgtagggg aagtgctccg cgtagaggca
     1441 cgccgggctg accttgcggt ccatgtagac gtagtcattg cccgagtgga agtcgatgag
     1501 gcatccaggc ctcgcccggt ccatggcctt gcgcgcacgc ttcatgatct cacggtcgta
     1561 gccgatgccg tccaggtaga ggccgtcgat gcccacgttg cggatgagcc acgccagccc
     1621 ctcgaggtag tagttgtgcc agcgcgacag gccgacggtg gcgatggcgg cgtcgatcct
     1681 cgcggggccg agaggttgat gccaggcggg gatgtagtgg tcgatcagat gctcttgtag
     1741 ccaggagtcc ttctgaccgc ccggaccgtc gacaaagatc tcgtcgccca ggctgcgcag
     1801 ggcccagatc tcggcgcaga agtcgctcag ctcccttacg gtgtaataga tcttcacctt
     1861 cagccccttc tcgtgagccc gtgtcacgta gtcgctcagc tcccgcgtct ggaggaaggg
     1921 gtagttgatg tgctggttca gatcgttgcc atggtggatg ttgatgacgg tggcaccgtt
     1981 ggcaacggcc tcttcgggag gggcgtacat gtggtagtag cgctggttcc aatgggccgg
     2041 gtcgagtggc ttcaccggtg tgatcagcag gccgaagcgg aactccagtt cctcgccagc
     2101 cctcaggctc cgccgtccgg tgtagactcg ggcgacaacg gtgtcgccct cctcgaacac
     2161 tgacgccccg cccttgccct cgttgtgcca cgatggcggg aaccccgagt ttcccaggtc
     2221 ccagctttgg tccgaggtct tcagccggca gtgaagcccg gcttgggccg tgccgatcca
     2281 gaagctgttg tgcggcgcgg tctcccactt ccattcccat cggtccggcc ggaagccgcc
     2341 cttgcgcttc agtcccatca tgtacgtggc ttggtcgcga cggaatggga tgtcgaggcg
     2401 cacatccgac acctcggtat cccggtccgc gcgcaggacg aggcggtagt tgaggtagcc
     2461 gtcgcactcg gcatccacat cgcaggagag ggccagaccc gaacccgcac tggcggcatg
     2521 gtaggcgacc ttgcccgggg cctggttcac gagccttggc agcccgccgg tccaccggcc
     2581 ctccaccccc cgccgctcga cggagaaggc catcggccgg gcgagtagat cgccgccggg
     2641 gcagtggatc tgcgcgggga ggccggtgtg atcgagtgtc accgaccggc ccagcactcg
     2701 cagtgtgttg cccgagacct cgacgggggt gtagggggcg accacttcat cgtcgaggcc
     2761 gatacgcgag ttgagccagg ccagtcgtga gtgattcgaa acattaccta ccccgccgtc
     2821 gggcgatacc tcgtcgagca cggtgaggcg aagcgtgacc tgcgtcgacg gtgcgttggc
     2881 gggcttcagg gtcaacggcc cctcgtaggc gcccgggcgg gcatcacggg gcacggcgaa
     2941 gacgaaccat agcgcctgca cccggccttg gtcgacgctc accgtcttcg cgatcggccg
     3001 gccgagccag tctgagcccg acgtgttgat gcacgtcagg cctcgggcgg ggaggaacgc
     3061 cccgccacct ccggccagcg gagcgggctc ggcggtgaga ccttcgatgg cctggcgtgc
     3121 ggcgtagagg ccaatctgga atgccaggag ctcgcccctc ttcgcctcgc accgaaacga
     3181 ggtgctcgga cccgcttcga tccagcggag cggcaggtca gaggccatgc ggatggggtg
     3241 cagtcggtcc tccgggaaga ccaggtacgg cttgccggga tgcgccgcga gcagggcttg
     3301 ggtctcggcc tcggtcgcga tcacctccat cgggtcgaac cgatggaagt cggtccgtgc
     3361 ctgaatggcc gtgacccgag cctccgggag cagcccgacc ccgccggcga cgcgttgccg
     3421 ccatgtcgga tcggcggtgt cctcgggctt gagatacgtg catgtgggcg agtactccca
     3481 cccggtggtc tcgaagggca tgtagtaggc gtagtactcg ccggccgtac gcgcctcgaa
     3541 gacgagatct ccggcctcgc gtgtgacgga cgccgccacg gtgttcgcta ctggctgacc
     3601 cgaggcagcc tcgacgacga tgacgcgctt ggcttcggga tccgggtccc ggcggcgcca
     3661 cgggatgcgc gcccgcaccg cctccgctgg ctcggagacc tgaatgaccg cccggtggtt
     3721 ccccagaccg tcgggccact cggccacttc gcggggcggc tcctcggccg ccaggttggg
     3781 cgggaccgcg ctcagaccaa tggcc
//
LOCUS       DAOSPL010000256         3802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_24151_length_3802_cov_2.973045, whole genome shotgun sequence.
ACCESSION   DAOSPL010000256 DAOSPL010000000
VERSION     DAOSPL010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24151_length_3802_cov_2.973045"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="PLQ54_06050"
     CDS             complement(<1..1382)
                     /locus_tag="PLQ54_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92855.1"
                     /translation="MAKFGVVAGRAVGPVACLVAIAGGWLYLLAPSPVPQRRPLTLPA
                     PRIAPTHEETVSPRLSDGRDWRAKLDRPIQWGHTWPGVDGRWQPAPEDRDDCDRVARV
                     CRAMGVPAIIQFAPRQRAMDGLTLHPGQSGHQALARALESVGGGDWSAQAPGVCIGIG
                     PGVGVPESLAGDTAVGPLTLVAPDAESMVRLLACAFDVDIAIEPSVLGKRRELIRAAS
                     VAHELWPLSMGDPAPALQCTIPRASRFPEILSSVAIYLGGEVDTSEGWTIRQWVTGQQ
                     LTAAAQSYVARLAEPSAATDPRLIEAIAVLGDVAIPVLEAALGGAERQVLVPAVQAMG
                     VIGSKRAARPVVDVLVWQPVCASVWEESIRLQAIRALGAVQSASARGLLLDLIRADQW
                     PQGTLEARVALGRIDPSAVPELPMGGTLAEPAGSVTENAIFSDGPLGRPRARGERRAA
                     AIVRRAGEAWA"
     gene            1654..3498
                     /gene="dnaK"
                     /locus_tag="PLQ54_06055"
     CDS             1654..3498
                     /gene="dnaK"
                     /locus_tag="PLQ54_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOS92856.1"
                     /translation="MGKVIGIDLGTTNSVVAVMEGGDPVVIPNKEGSRTTPSVVGFTP
                     QGERLVGAPAKSQAVVHPDRTISSIKRKMGTRDRITIDGKEYSPEQISAMILQKLKAD
                     AEAYLGEKVTQAVITVPAYFDDAQRTATKNAGEIAGLEVLRIINEPTAASLAYGLDKK
                     EDETILVYDLGGGTFDVSLLEVGGGVFEVKATSGDTRLGGDDFDDRIVTWLADEFQKQ
                     NGIDLRKDRQALKRLKEAAEKAKCELSSTVTTTITLPFITADQDGPKHLEMTLTRAQL
                     EEMLGELFAKTLGPFRQAMKDAGLSKSDIDEVVLVGGSTRIPAIQDIVRREAGREPCK
                     GVNPDEVVAVGAAIQGAVLAGEIKDVVLLDVTPLSLGIETLGGVFTKLIDRNTTIPTH
                     KAETFTTAADGQTSVEVHVLQGEREMARFNKTLGRFQLVGIPPAPRGVPQIDVSFDID
                     ANGILNVSAKDKATGKEQRITITGTGQLSDEEIERMVKEAEQHADEDRRMREVADVRN
                     TADSLIYQTERTMKDLGSEVSDDDRRRVEDALQKVKDLKEKEDVPALKSAIEELQQAS
                     YAMSEVLYRRATAQAEAGAGASGDGSGAEAPSEAGGTDDVIDAEFKPK"
     gene            3576..>3802
                     /locus_tag="PLQ54_06060"
     CDS             3576..>3802
                     /locus_tag="PLQ54_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92857.1"
                     /translation="MSDDTAKGTTRKRTTARATAKTTAAGGSRTRKRTAPARPADENT
                     ASAQAVGAAAEAEPQGAEGEEVATRPFWDRKA"
BASE COUNT          707 a         1317 c         1260 g          518 t
ORIGIN      
        1 gcccacgcct cgccggctct gcgcacgatg gcggccgccc gacgctcgcc acgggcacgc
       61 ggccggccca gcgggccgtc gctgaaaatg gcgttctcgg tcaccgatcc cgccggctcg
      121 gcaagcgtgc cgcccatggg gagctccgga actgccgagg gatcgatgcg gcccagggcc
      181 acgcgcgcct caagagtgcc ttgcggccac tggtccgccc ggatcagatc gagcaggagc
      241 ccgcgcgcac tggcgctctg cacagccccc agcgcgcgga tggcctgcag gcgtatggac
      301 tcctcccaca cgctggcgca gaccggctgc cacacgagta cgtccaccac cggtctggcc
      361 gcccgcttgc tcccgatcac acccatggcc tggacggccg gcacaagcac ctggcgctcc
      421 gcaccgccga gggccgcctc aagaaccgga atggcgacat caccaagcac cgcgatggct
      481 tcgatgagcc gcggatccgt cgcggcactc ggctcggcca gccgtgcgac atagctctgc
      541 gccgccgcgg tcagttgctg cccggtgacc cactgccgta tggtccagcc ttcactcgtg
      601 tccacctcgc cccccaggta gatcgccacg ctcgagagga tctcggggaa ccggctcgcc
      661 cgggggatgg tgcactggag agcgggggcg ggatcaccca tggacagcgg ccacagctcg
      721 tgggccaccg acgccgcccg gatcaactct ctccgcttac caagcaccga gggctcgatg
      781 gcgatgtcca catcgaacgc gcaggccagc agccgcacca tcgactcagc gtccggagcg
      841 accagggtca gcgggccgac ggccgtgtcc ccggccaggc tctccggcac acccacgccc
      901 ggcccgatgc cgatgcagac cccgggcgcc tgggccgacc agtccccccc gccgacactc
      961 tccagcgccc tcgccaaagc ctggtggccg ctctgccccg gatggagtgt cagcccgtcc
     1021 atggcccgct gtctcggtgc gaactggatg atggcgggca cacccatggc ccggcagacc
     1081 cgcgctaccc ggtcacagtc gtcgcgatcc tcgggggccg gctgccaccg gccatccacg
     1141 cccggccagg tatgccccca ctggatgggc cgatccagct tcgcacgcca atcgcgacca
     1201 tcggacagac ggggcgagac ggtctcctcg tgggtcggtg cgatgcgtgg tgccggcagt
     1261 gtcagaggtc ggcgctgcgg aaccggcgag ggcgccagca ggtagagcca gccgccggca
     1321 atggcaacga ggcacgcgac cgggcccaca gccctacccg ccacgactcc gaacttcgcc
     1381 actccacgac tccttgaacg gggcggtgcc gagcggcgcc ccaatcggca acctcgggac
     1441 gcacttcggc gccgccctcc ggctgccctc ccctgctcgc acccataact atgattggac
     1501 caagatagat tgcttgacag tattgcactc aagtcttata gtaacggtgg ttcgcggtgg
     1561 cggcgccacc acggaccctg ggtgcagtca cccatcgatc gcgctatcca gggcgcagag
     1621 aaggcgccct gtgacctggg atggtgagcg gcattgggaa aggtcatcgg aattgacctc
     1681 gggaccacca actcggtggt cgccgtcatg gagggtggcg accccgtcgt tatcccgaac
     1741 aaggaaggga gtcgaacgac gccttctgtt gtgggcttca cgccgcaggg cgagcggctg
     1801 gtgggtgcgc ctgccaagag ccaggccgtg gtgcatcccg atcgtaccat ctcgtccatc
     1861 aagcgcaaga tgggcacccg tgaccggatt accatcgatg gtaaggagta ctcgcccgaa
     1921 cagatctcgg ccatgatcct ccagaagctc aaggccgatg ccgaggccta cttgggggag
     1981 aaggtcactc aggcggtcat caccgtgccg gcgtatttcg acgacgccca gcggacggca
     2041 acgaagaacg ctggcgagat cgcgggcctc gaggtgctcc gcatcatcaa cgagcccacc
     2101 gccgcgtcac tcgcctacgg actcgacaag aaggaggatg agaccatcct ggtctatgac
     2161 ctgggtggcg gcacattcga cgtctcgttg ctcgaagtgg gcggcggcgt cttcgaggtc
     2221 aaagccacct cgggcgacac gcgcctgggc ggcgacgact tcgacgaccg catcgtcacc
     2281 tggctcgccg atgagttcca gaagcaaaac gggatcgacc tgcgcaagga ccgccaggcc
     2341 ctcaagcgcc tcaaggaggc ggccgagaag gccaagtgtg agctctcgag cacggtgacc
     2401 acgaccatca ccttgccgtt catcacggcc gatcaggacg gcccgaagca tctcgagatg
     2461 accctcaccc gggcccaact cgaggagatg ctgggcgagc tgttcgcgaa gaccctcggg
     2521 cctttccgac aggccatgaa ggacgcgggg ctgagcaagt ccgacatcga cgaggtcgtg
     2581 ctcgtaggcg gttcgacacg catccccgcc atccaggaca tcgtccggcg ggaggccggt
     2641 cgcgagccgt gcaagggcgt gaaccccgac gaggtggtcg ccgtgggagc cgccattcag
     2701 ggcgccgtgc tcgccggcga gatcaaagat gtcgtgctgc tcgacgtgac gccgctctct
     2761 ctgggcatcg agaccctggg cggagtgttc accaagctga tcgaccgcaa cacgacgatc
     2821 ccgacccaca aggcggagac gttcaccacg gccgctgacg gccagaccag tgtcgaagtc
     2881 cacgtgctgc agggcgagcg cgagatggcg cgcttcaaca agaccctggg gcggttccag
     2941 ctggtgggta tccctcccgc gcctcggggc gtgccgcaga tcgacgtgtc gttcgatatc
     3001 gacgccaacg gcatcctgaa tgtgtccgcc aaagacaaag cgacgggcaa ggagcagcgc
     3061 atcaccatca cgggtaccgg tcagctctcc gatgaggaga tcgagcggat ggtcaaggaa
     3121 gccgaacagc atgccgacga agaccgtcgc atgcgcgagg ttgccgatgt gaggaacacc
     3181 gccgactcgc tcatctacca gaccgagcgc accatgaagg acctgggctc ggaagtgtcg
     3241 gatgacgacc gccgccgtgt cgaggatgct ctgcagaagg tcaaggacct gaaggagaaa
     3301 gaggacgtcc cggcactcaa gtcggcgatc gaggagctcc agcaggcctc atatgccatg
     3361 tccgaagtgc tgtaccgccg ggccaccgcc caggcggaag ccggggccgg tgcctcgggg
     3421 gacggcagcg gcgccgaggc gccgtcggag gccggtggca ccgatgacgt gatcgatgcg
     3481 gagttcaagc ccaaatagac ggcgcggcca aggcgccgtc cttcgcggac acggcagagt
     3541 ctgcgtgagc cgcggcgaat gcgagagggg ttaccgtgag cgacgacaca gccaagggca
     3601 ccacgcgcaa gcgtaccacg gccagagcca ccgccaagac gacggcggcc ggcggttccc
     3661 gcactcgcaa gaggacggcg cccgcccggc cggccgacga gaacaccgct tccgcgcagg
     3721 ccgtcggcgc agcggctgag gcagagccgc aaggcgccga gggcgaagag gtcgccacgc
     3781 ggccgttctg ggatcgcaaa gc
//
LOCUS       DAOSPL010000257         3802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_24153_length_3802_cov_2.480117, whole genome shotgun sequence.
ACCESSION   DAOSPL010000257 DAOSPL010000000
VERSION     DAOSPL010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24153_length_3802_cov_2.480117"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..1184)
                     /locus_tag="PLQ54_06065"
     CDS             complement(147..1184)
                     /locus_tag="PLQ54_06065"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOS92858.1"
                     /translation="MNDIAWRMAVIDPDTEGRSFAQRAFEDRGWVVQSFDGGAEAVAA
                     CRQKPFDVVLLDLGITDVDGLDVLRDLREACVDTEVIVVTSHVSLDSAIRALELDAYA
                     YIAKPVEISDLVRSVENAQDKIALQRLNSELIEHLCRNQAELERAIEEATRELARANA
                     RLQEMAVRDGLTNLYNRRYFDERLGDEVSRSKRYGSRLTLVLLDIDHFKAHNDAYGHL
                     RGDDALITLAQVLQDNVRRNDVVARFGGEEFAILLIEATEDHVATVCERIRSAVEATA
                     FMGHTPSHSVSLTVSLGYASCPTDTSDMAGLIESADEALYAAKASGRNCIRSYAGGRL
                     IDPLVGTDAAG"
     gene            complement(1305..3689)
                     /locus_tag="PLQ54_06070"
     CDS             complement(1305..3689)
                     /locus_tag="PLQ54_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92859.1"
                     /translation="MFARGSVRIWAPAVLAWAVLMCSAQAEETSAGVRPLALPRPVFL
                     AHGVPSLVDLARYQDYGFNAVYVVLSPPLEGDARTRLLDMLKACEELALPVIVELGAA
                     PHGTMSLVSPYDPEYVVRADEWIGGMVRDLRRHAVICAWAVASGASRVYTPDDVDFRE
                     YLTQRYETLTALRQAWGATLPTWDSVSLARATPLGASPMLSVSRSALDAAEYRMSAQR
                     ALLAQWLQTIRQSDPSRPVWVGSEASPAALACVPEGFAGVFVAPPVGTEGADWRDFLP
                     AVALGRRGGRLDAIIEVPARRAVIESYRALVLQALAQGAAGVALDSWRWARTNAAIRD
                     GLRDAREVCAEQDMGRFEPRPSVAVYHLPYADGTVTPEGDGLFGYLAGFSPGEPSLLA
                     STYAMGHRYGLVDYIGPESVLAGRLRQYGVVLAPHCVDLPPQIIRAFMDFVNAGGILV
                     LDIGAGVAQTNNFLLFPPGLEMLTGVTRLGRVFMAPMNVTVKDVPPPFRSLPPNAVSM
                     GVGGGAAFTGGIGDAILSLGARVVAESHGRRAELGHLYRGGILANSFGAGYCVFCTGR
                     LFASWSPDDPLYQAVFADLFAHGARIENVTAEGLFDRALTVSGGANGFIVHNLSDRPL
                     KARLRLSAAEGMLTRSVWMHYPSASAGRAAVDAVADLAAGQCLYAAAVPVTIEGTSWW
                     ACVERYGPDEVRLQLVNGQAPDPLADHHLAAGPDAQPPAGDLEIRVASGLYAVEPSSR
                     HFVTVRSASGGGMAEAARLVTADDKGVIHLSVGSGGGTVILRSESTGVPGSDGG"
BASE COUNT          713 a         1323 c         1226 g          540 t
ORIGIN      
        1 acgccaatgc ctgaaggttc gcacgggccg tccgcttggc ttcaggggaa ccgggcagtg
       61 agagcgacgc gccggccgac tgggaccgga tggtccgtat gcggtacggg caggccgccc
      121 aatccgccgt caggcgccga ggcagatcag ccggccgcgt cggtgccaac cagcggatcg
      181 atcaggcgtc cgccggcgta cgaccgaatg cagttgcgtc cactcgcctt ggcggcatag
      241 agcgcttcgt ccgccgactc gatcagcccg gccatgtcgc tcgtatccgt ggggcacgac
      301 gcgtaaccca gactcaccgt caggctgacc gagtgcgatg gcgtgtggcc catgaaagcc
      361 gtcgcttcga cggcggaccg aatgcgctca cagaccgtgg cgacatggtc ctcggtcgcc
      421 tcaatgagaa ggatagcgaa ctcctcgcca ccaaaccgcg ccaccacgtc gttgcggcgc
      481 acgttgtcct gaagaacctg ggcgagcgtg atcagagcat cgtcgccgcg aaggtgcccg
      541 taggcatcgt tgtgtgcctt gaagtgatca atgtcgagga gcaccagtgt cagccgagaa
      601 ccgtaccgct tcgagcgcga gacctcatcg ccgagccgct cgtcgaagta gcgccggtta
      661 tagaggttag tcaagccgtc gcgaacggcc atctcctgga ggcgggcgtt ggcacgcgcc
      721 agttcccgag tcgcctcctc gatggcgcgc tcgagttccg cctggttgcg gcacaggtgc
      781 tcaatcagct ccgagttcag gcgctgtagg gcgatcttgt cctgggcgtt ctcgactgac
      841 cggaccaggt cgctgatctc gacgggtttg gcgatgtagg cgtatgcgtc gagctcgagg
      901 gcgcggatcg ccgagtcaag cgacacgtgg ctcgtgacca cgatcacctc agtgtccaca
      961 catgcctctc gtagatcgcg cagcacatcc aggccgtcga cgtccgtgat ccccagatcc
     1021 agcaggacta cgtcgaaagg cttctgccgg cacgcggcga cggcctcggc ccctccgtca
     1081 aatgactgga caacccagcc tcgatcctca aacgcgcgtt gggcgaagga gcggccttca
     1141 gtatcggggt cgatgaccgc catacgccag gcgatgtcat tcatgcaaag actcccgcga
     1201 ccgagctcct ccccgatcac ccgagccaca gacagagtcg ctgccacacg atgactcggt
     1261 acgggctcac tgggcacccg ccgccgctcg ggcagccgag gcgctcatcc accgtcagaa
     1321 cccggtaccc ctgtgctttc ggaccgcagg atcacggtcc ctccgccgga gcccacagaa
     1381 aggtggatga ctcctttgtc gtccgccgta accagccggg cggcttccgc catgcccccc
     1441 ccggaagcgg accggaccgt aacgaaatgc ctggacgatg gctcgacggc atagagccca
     1501 ctcgcgacac ggatctccag gtcgcctgca gggggctgtg cgtcgggccc tgccgccaga
     1561 tggtggtccg ccagcggatc gggggcctgc ccgttcacga gctgcaggcg gacctcatcc
     1621 ggaccatagc gctccacgca cgcccaccag gacgtgccct cgatagtcac gggcaccgca
     1681 gccgcgtaga gacactggcc tgccgccaga tcggcgacgg cgtccaccgc cgcacgtccc
     1741 gccgaagcag acggatagtg catccagacc gagcgtgtca acatgccctc cgcagccgag
     1801 agcctcagcc gagccttaag cggcctgtcg gagagattat gaacgatgaa gccattggcg
     1861 ccacccgaga ccgtcagcgc tcggtcgaac aggccttcgg ccgtgacgtt ctcgatccgg
     1921 gcaccgtgag cgaagaggtc ggcaaacacc gcctggtaga gggggtcatc gggcgaccag
     1981 gatgcgaaga gcctcccggt gcagaacacg cagtaccccg cgccgaagga gttcgccagg
     2041 attcccccgc ggtacaggtg acccaactcg gcgcgacgac catgggactc agcgaccacg
     2101 cgcgcaccca ggctcagaat ggcgtcccca atccccccgg tgaacgcggc tcctcccccg
     2161 acgcccatcg acacggcgtt ggggggcaaa gagcggaacg gcgggggcac atccttcacc
     2221 gtgacattca tcggggccat gaacacgcgt cccagacgtg tcacgccggt cagcatctcg
     2281 aggccggggg ggaacaggag gaagttgttc gtctgagcca cgcccgcgcc gatgtcgaga
     2341 accaggatcc cgcccgcgtt gacgaagtcc atgaatgcgc ggatgatctg tggtggcagg
     2401 tcaacgcagt gaggggcgag gaccacaccg tactgccgaa gccggccagc gagcacggat
     2461 tcggggccga tgtagtccac cagcccatag cggtggccca tggcgtaggt ggaggccagc
     2521 agagacggct cgcccggtga gaatccggcc aggtagccaa acaagccatc gccttccggg
     2581 gtgaccgtcc cgtcggcgta cggcaggtgg tagacggcca ccgagggccg cggctcgaag
     2641 cggcccatgt cctgctcggc acacacctcc cgggcatccc gcagcccgtc gcggatggcg
     2701 gcgttcgttc tcgcccaacg ccacgagtcg agggccacgc cggccgcgcc ctgtgccaat
     2761 gcctgcagca ccaaagcgcg gtaactctcg atcacggccc gtcgcgcggg tacctcgatg
     2821 atggcatcga gccgcccccc gcgtcgcccc agcgccacag caggcaggaa gtcgcgccag
     2881 tcggcgcctt ccgtgccgac gggtggcgcc acaaacacgc cggcgaaacc ctcgggaaca
     2941 caggccagag ccgcgggact ggcctcggag cccacccaga cagggcggga tggatccgac
     3001 tgcctgatgg tctggagcca ctgggccagc agggcgcgct gggcggacat gcggtactcg
     3061 gcggcgtcca gcgccgatcg cgacacactc agcatcggag acgcgcccag tggcgtggca
     3121 cgcgcgagcg acacggagtc ccaggtgggc aacgtcgcgc cccacgcctg ccgaagtgcg
     3181 gtcagggtct catagcgctg ggtaaggtac tcacggaagt ccacgtcatc cggcgtgtac
     3241 acacggctcg cgcccgaggc cactgcccac gcgcagatca ccgcgtgacg ccgcaggtcg
     3301 cggaccatgc ccccaatcca ctcatcggcc cgcaccacat actcggggtc atagggcgag
     3361 accaacgaca ttgtgccgtg cggggccgct cccagctcca cgatcacagg cagcgccagc
     3421 tcctcgcaag ccttgagcat atccaggagg cgagtgcgag cgtctccctc cagaggcggc
     3481 gagagcacaa cgtagacggc gttgaagccg tagtcctgat agcgcgcgag atccacgagc
     3541 gagggaacgc cgtgggccaa gaacacgggc ctggggagag cgaggggacg gacaccggcc
     3601 gatgtctcct ccgcttgagc ggagcacatc agcacagccc acgccagcac cgcaggcgcc
     3661 catatcctga cggagcccct cgcgaacacg atcaagcctc ctgatgcccg cagccggagt
     3721 gatcgcggag cacacgaatc gccatacgag ccgcctcgcg ctcggcctcc ttcttgctgc
     3781 ggccactgcc agtccccatg at
//
LOCUS       DAOSPL010000258         3799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_24185_length_3799_cov_2.809028, whole genome shotgun sequence.
ACCESSION   DAOSPL010000258 DAOSPL010000000
VERSION     DAOSPL010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24185_length_3799_cov_2.809028"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLQ54_06075"
     CDS             complement(<1..350)
                     /locus_tag="PLQ54_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HOS92860.1"
                     /translation="MLTVVHSAAVFGIDAYGVRVEVDLSPGLPHVTLVGLPDGAVKES
                     VDRVFVALKNSSFETPSLRLTVNLAPADRRKDGPAFDLPIAMGILASSGQIRADCMAD
                     VGFTGELSLDGGIRR"
     gene            complement(392..2287)
                     /locus_tag="PLQ54_06080"
     CDS             complement(392..2287)
                     /locus_tag="PLQ54_06080"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOS92861.1"
                     /translation="MRHRAGSVLVLLPVLVSGQSPAEAAGAALDPARVEAIVAMLPEA
                     PTGLGRPADDRAAWQELAARPAFQGVVARAEEIAASPLPDLPDELFLDFSRTGNRRRW
                     EMVNNSRRGRLAPLVLGECIEDGGRFIAPIEELVRALCAEPTWVMPAHDGTLANFEGK
                     VIDIDLASSALGRELATAAYLLGDRLSEDTRALLRDNLTRRIFDPFIAMVRGDRTPNW
                     WLLTTNNWNAVCLAGVTGAALAGLESRGDRALVVAAAEALSLNFLKGFTADGYCSEGV
                     GYWNYGFGHYVELAETIGQATGGQIDLFERDAAKSAASYATRIQIVNGVCPAFADCSI
                     TEQPSAQLVEFLDRRYGWGLRQYEDNDMSTSGGGLSSSMLYSFPNSATLAPPAPAAAA
                     GPGLRSWFEQAGILICRPAPGGDRPFAVALKGGHNEEHHNHNDVGSYVVVLGDQPVLL
                     DPGAEVYTKRTFSAQRYDSSLLNSFGHPVPLVGGQLQRAGREARADVLAAEFSDAADT
                     LTLDITSAYRVEGLRTLTRRFTYSREGSGSLEVSDHATFVSPQTFETALITRGTVEQS
                     GPTTLRVTDGGQSVEVEIRAPGDFALVTDDLRDDSKEEPVAKRVGIRLVAPVTDATVT
                     ATIRPAE"
     gene            complement(2324..3259)
                     /locus_tag="PLQ54_06085"
     CDS             complement(2324..3259)
                     /locus_tag="PLQ54_06085"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOS92862.1"
                     /translation="MPDSRIQALIGQVAYDRLRHDLFALCQDPLPFRKLNYTVPGHEK
                     CSLYEADDFIAAELAAAGYAVEREPCQVQCYRCDSSKPKAHQYSPPEPDDPWYTAYNL
                     LARKAGTDRADEIIVIVAHKDSQSWVDSPGALDNAVGTVGALEIARLVAGQTLRRSVW
                     LIFCNEEHTPWTSAAAAQACRDRGDNLVAIVNLDAIAGRTQEAIDQGIPAAFTAYTEE
                     PGRAIAEHIHRVNEEYGIGLVHTIARREFANDDDGSFVKAGFPAAVVHIGSWPYGDPN
                     YHTEEDTPERVDVRVVQMSIQAVLGTVLEIAELCR"
     gene            complement(3372..>3799)
                     /locus_tag="PLQ54_06090"
     CDS             complement(3372..>3799)
                     /locus_tag="PLQ54_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92863.1"
                     /translation="VERDLKQKANQKKVTACLVIVPPEVGFEIVGRGGVGVEVLEKDL
                     GVALYVRTDPNLTADQYRIEAGARQDIEQQLRILRHGQMFHVRPTKAEIDADTVTLAS
                     VDGMMVEIDTNKDPEDLGDLVTVSLSDIGRSWARGTLLE"
BASE COUNT          629 a         1268 c         1264 g          638 t
ORIGIN      
        1 cgccggatac cgccatcgag cgaaagctca cccgtgaaac ccacatcggc catgcagtca
       61 gcccggatct ggccgctcga ggccaggatc cccatggcga tgggcagatc gaacgcaggc
      121 ccatccttgc gtcgatcggc cggcgccaag ttcaccgtca gccggaggga aggcgtctcg
      181 aagctcgagt tcttgagggc cacgaacaca cggtcgacgc tctccttgac cgcgccgtca
      241 ggcaggccga ccagcgtgac gtgcggcaga cccggcgaca ggtcgacctc gacacgcact
      301 ccgtaggcat cgatgccgaa cacggcagcc gagtgaacaa ccgttagcat gggaaccagt
      361 tcgggccgcg aacgcccgtt ccctccccgc cctactccgc gggccgtatc gtggccgtca
      421 ccgtggcatc ggtcactggg gcaaccagcc gaatgccgac ccgtttcgcc acaggctctt
      481 ccttcgagtc atccctcagg tcatccgtca ccagggcgaa gtcgcccggc gcccggatct
      541 cgacctcgac cgactggccc ccatcggtga ccctgagcgt ggtcggcccg gactgctcga
      601 cggtcccccg agtgatcagt gcggtctcga atgtctgggg cgaaacgaac gtggcatgat
      661 cgctcacctc gagcgagccc gagccctcgc gcgagtaggt gaaccgccgc gtcagcgtcc
      721 gcaggccctc aacgcggtac gcggacgtga tgtcaagggt gagggtatcg gcggcgtccg
      781 agaactcggc agcgagcacg tcggctcggg cctcccgtcc tgcccgctgg agctgaccac
      841 ccacgagtgg cacgggatgc ccaaacgaat tgaggagtga gctgtcatag cgctgcgcgc
      901 tgaacgtccg cttggtgtac acctcggcac ccgggtcgag cagcaccggc tggtcgccca
      961 agacgacgac gtaggagccc acatcgttgt ggttgtggtg ctcctcgtta tgcccgccct
     1021 tgagggccac ggcaaacggg cggtcaccgc ccggcgccgg acggcagatc aggatgccgg
     1081 cctgctcgaa ccatgaacgg aggcccgggc ccgctgcggc cgctggggcg ggtggtgcca
     1141 gcgtggccga gttcgggaag ctgtagagca tcgagctgct caggcctccg ccgctggtgc
     1201 tcatgtcgtt gtcttcatac tgcctcaatc cccagccgta gcggcggtcg aggaactcca
     1261 cgagctgtgc cgagggctgc tcggtaatgc tgcaatccgc aaatgccggg cacacgccgt
     1321 tgacgatctg aatgcgggtg gcgtagctgg ccgccgactt ggccgcatca cgctcgaaca
     1381 agtcgatctg ccccccggtg gcctggccga ttgtctcggc cagctccaca tagtggccga
     1441 agccgtagtt ccagtacccg acgccctcac tgcagtaccc gtcagcggtg aagcccttga
     1501 ggaagttgag cgagagtgcc tcggctgcgg cgacaacgag cgcgcggtca ccgcgcgact
     1561 cgaggccagc cagcgcagcc ccggttaccc cggccaggca caccgcgttc cagttattgg
     1621 tggtgagaag ccaccagttc ggcgtccggt caccacggac catggcgatg aacgggtcga
     1681 agatgcgtcg cgtcaggttg tcgcgcagca gagcccgggt gtcctcactc agacggtcgc
     1741 ccagcagata cgccgcggtc gccagttccc ggcccagcgc cgacgaggcc aggtcgatgt
     1801 caatgacctt cccctcaaag ttggccaggg taccgtcgtg ggcgggcatc acccaggtgg
     1861 gctcggcaca caaggcccga acgagttcct cgatcggggc aatgaaacgc ccgccgtcct
     1921 cgatacactc accgaggacc agaggcgcca gccgaccgcg acggctgttg ttgaccatct
     1981 cccaccggcg gcggttgccg gtccgcgaga agtcgaggaa gagctcatcg ggcaggtcgg
     2041 gcagtgggga ggccgcgatc tcctcagcgc gagccaccac gccctggaat gcaggccggg
     2101 cggccagctc ctgccacgcc gcgcgatcgt cggccggtcg gccgaggccc gtgggggcct
     2161 ccggaagcat cgcgacgatg gcctcgacgc gggccgggtc cagcgccgcg cccgccgcct
     2221 cggcgggcga ctgaccggag accaggaccg gaaggaggac cagcacgctg cctgctcggt
     2281 gtctcattga cagccacctc cgcgaggagt caggtcgcga cctctagcgg cagagctcgg
     2341 cgatctccag aaccgtgccc aggaccgctt gtatagacat ctgtacaact ctcacatcga
     2401 cgcgctccgg cgtgtcctct tcggtgtggt agttcggatc accgtagggc cacgagccaa
     2461 tgtggacgac ggccgccggg aacccggctt tcacgaacga cccgtcatca tcgttcgcga
     2521 actcccgccg cgcgatggtg tggaccagcc cgatgccgta ttcctcgttc actcggtgga
     2581 tgtgctccgc gatggcccgg ccaggctcct cggtgtaggc ggtgaatgct gccgggatgc
     2641 cctggtcgat ggcctcctgg gtgcggccgg cgatggcgtc gagattgacg attgccacca
     2701 ggttgtcgcc tcggtctctg cacgcctggg ctgcggccgc gctggtccag ggggtgtgct
     2761 cttcattaca gaagataagc catacactgc gccgtagcgt ctggcccgcg acgagccggg
     2821 cgatctcgag cgcgccaacg gtgcccaccg cattgtccag cgcgccaggg gagtccaccc
     2881 agctctgcga gtccttgtga gcgacgatga cgatgatctc atcggctcga tccgtgcccg
     2941 cctttcgcgc gagcaggttg taggccgtgt accacgggtc atcgggctcc ggcggcgagt
     3001 actgatgcgc tttgggtttg gagctgtcgc agcggtagca ctggacttgg cagggctcgc
     3061 gctcgacagc gtacccggcc gcggcgagct ccgcggcgat gaagtcatca gcctcgtaca
     3121 gcgaacactt ttcatgcccg ggcacggtgt agttgagctt gcggaagggc agagggtctt
     3181 ggcagagcgc gaagaggtca tggcgcagcc ggtcgtaggc gacctggcca atcagggctt
     3241 ggatccgtga gtcaggcatg gggctcccct tcggtgaacg tcgtctcgag gatttcgcgc
     3301 ccagcggcac atggccctgc cgatccgcgg cgcgtgtctt cagccatgcg cgccgcggat
     3361 cggcgagcca gctactcgag cagagtgccc cgagcccatg agcggccgat gtccgagagg
     3421 gacacggtga caaggtcgcc gaggtcttcg ggatccttgt tggtgtcgat ctcgaccatc
     3481 atcccgtcga cgctggccaa ggtgaccgtg tcggcgtcga tctcagcttt ggtcgggcgg
     3541 acatggaaca tctgcccgtg gcgcaggatg cggagctgct gttcgatatc ctgccgggct
     3601 ccggcctcga tgcggtactg gtccgcggtg aggttcgggt cggtgcgcac atagagggcg
     3661 acgcccaggt ccttctcgag cacctcgacg cccacaccgc cgcggcccac aatctcgaag
     3721 ccgacctcgg gtggtacgat gacgaggcat gcggttacct ttttttgatt tgccttctgt
     3781 ttcaggtcgc gctcgacgc
//
LOCUS       DAOSPL010000259         3786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_24291_length_3786_cov_2.946127, whole genome shotgun sequence.
ACCESSION   DAOSPL010000259 DAOSPL010000000
VERSION     DAOSPL010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24291_length_3786_cov_2.946127"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PLQ54_06095"
     CDS             <1..328
                     /locus_tag="PLQ54_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92864.1"
                     /translation="YAWAACTVALAGYSALWGASTHPRGQGGTGRALSCLITLHLCHW
                     WYVSAVRGALDQVAHAPSGSVWQQAGLYLPMLAVALGLALLARRGAALGVERRKRVNP
                     YGPADA"
     gene            complement(422..1477)
                     /locus_tag="PLQ54_06100"
     CDS             complement(422..1477)
                     /locus_tag="PLQ54_06100"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS92865.1"
                     /translation="MEPVAVGIVGCGVIGSVHVDVASKLDTVKVVAVADLRQDRARSA
                     ADQYGIGTVHPDGDSLIDDPQVEAVILALPANQRTYMGLRAFARGKHVLTEKPVAMNA
                     DEVRRLIAAKGNLVGACCSSRYAFSEATRVAKEFVASGALGQLRLIRCRSVNCPGKRV
                     EGERPYWRVSQSSNAGGILVNWGSYDLDYLMNLTGWSLRPVAALGALWPAGPRYQWFV
                     APGSDAETHVTALVTFEQGVALAYERGDAVAGSHDETWEIVGDCGTVHLKMTSGSGKR
                     LTWDQADTEQGLTSHVLWEGDDDGSTVHSGPVDDFAKAIRQGRPPQTGLEQALLVQRI
                     CDAIYRSASTGTAAPVV"
     gene            complement(1778..3328)
                     /locus_tag="PLQ54_06105"
     CDS             complement(1778..3328)
                     /locus_tag="PLQ54_06105"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOS92866.1"
                     /translation="MRAHAVVGIAVVAVASGAAFGAPSVIVEGTEAPTALWASNDTPG
                     AMPDCRVEWTLIYADGSHYTEAAIVDLPAEGSVQVSDCREWWDERRSASLTVVTELFD
                     AGGARLSGSAYGPFRSVPRRGLPVDGWTAKSSRGAGEDLAFDGNPQTRWDTGAKQRSG
                     DWYLLDLGRTQQVSGVILDARGSAEDYPGGLRVEGSTDGKTWQSLAEIADVPAINRGG
                     LVRVEFDPTPIRAVQLTLTAPHGDSWFWSIHELSVLSPDAPEASAPAGAAEEPVVAKA
                     APAVAPEKSVLYFSCTRGFRHSAAPYMKPILTRLGEENGFSVLCSEDPRMISKERLAG
                     LDCVIFGNTTGSFLNDSQKVALLDFVSAGGAFVGIHAATDTHYDWPSYRDLVGGWFDG
                     HPWNEDVKLLVEVPDHPACEPVPQGWVVADEIYQQRDWSRDDVCVLMSLDPSGTDMTK
                     PGIHRKDGDFGIAWCKRYGKGRSFYTALGHREEVCDSPVYQAHLLGGMRWAMGQAEGT
                     STPHPRPE"
     gene            complement(3361..>3786)
                     /locus_tag="PLQ54_06110"
     CDS             complement(3361..>3786)
                     /locus_tag="PLQ54_06110"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004378330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOS92867.1"
                     /translation="APGTTEPSGQADTKAQAPAEGESSAIGKRVTTASGLQYEDIKVG
                     TGKSPKMGDSVTVDYRGTLKDTGKEFDSSYERGQPATFTLGQVIEGWNEGLQTMKEGG
                     KRKLIIPAKLGYGERGAPPDIPPNADLVFEVELLEVTPR"
BASE COUNT          590 a         1343 c         1260 g          593 t
ORIGIN      
        1 ctacgcctgg gcggcctgca cggtggccct ggcggggtac tccgccctct ggggcgcgag
       61 cacgcacccc aggggccaag gggggaccgg acgtgccctg tcctgcctca taactctgca
      121 cctgtgccac tggtggtacg tgtcagccgt ccggggtgcg ctcgaccagg tggcgcacgc
      181 accatcgggc tcggtatggc aacaggccgg gctgtacctg ccgatgctgg ctgttgccct
      241 gggactcgca ctactggcgc ggcggggggc ggcgctgggc gtggaacggc gcaagcgtgt
      301 caatccctat gggccggcgg acgcgtaggt cgggcgtccc gcccgacacc gatcacgagc
      361 cgaccgagac gctcggcccg cgagcgcgct cggcgaccgg aacggtcgcc ctacgcggcc
      421 cctacaccac gggcgccgcc gtgcccgtgc tcgccgagcg gtagatggcg tcgcagatgc
      481 gctggacgag cagggcttgc tcgaggcccg tctgcggcgg ccggccctgg cggatggcct
      541 tggcgaagtc gtccactggc cccgagtgaa ccgtcgatcc gtcgtcgtca ccctcccaca
      601 gcacgtggga ggtcagcccc tgctcggtat cggcctgatc ccaggtgagc cgtttccccg
      661 agccgctggt catcttcagg tggacagtgc cgcagtcacc gacgatctcc cacgtctcgt
      721 catggctccc cgcgacggca tctccgcgct cgtaagccag agcgacaccc tgctcgaacg
      781 tgaccagggc cgtcacatgc gtctcggcat cggatccggg cgccacgaac cactggtacc
      841 ttggtccggc gggccagaga gcgcccaggg cggccaccgg ccggagcgac cagccggtca
      901 ggttcatgag gtagtcgagg tcgtacgacc cccagttgac gaggatgcca cccgcgttgg
      961 atgactggct gacgcgccaa taggggcgct cgccctcgac gcgcttcccc gggcagttca
     1021 ccgagcggca gcggatgagc cggagctgac cgagggcacc cgacgcgacg aattccttcg
     1081 ccacccgcgt cgcctcggag aaggcatagc ggctcgagca gcacgcgcca accaggttcc
     1141 ccttggccgc gatgagccgg cgcacctcgt cggcgttcat ggccaccggc ttctcggtca
     1201 gcacgtgctt gcctcgggcg aaggcgcgca gacccatgta agtgcgctgg ttggcgggga
     1261 gggccagaat gaccgcttcg acctgcgggt cgtcgatcag gctgtcgcca tcggggtgca
     1321 cggtgccgat gccatactgg tcggccgccg agcgggcgcg atcctgccgc aggtccgcga
     1381 ccgccaccac cttcaccgtg tcgagcttcg atgccacatc gacgtggacg ctcccgatga
     1441 ctccacaacc cacgatgccg acggcgactg gctccactgg actcgaacct cccatgtctg
     1501 ggtgcgctga acgcggccgg tgttcgcccg aaggagcgac ggctcctgtg agcagcgcgc
     1561 cgggggcagt gatctcgccc gatcgcgttc ggccgatatg cggccaggag gagacaaagc
     1621 aagaagcgag ccggagacgt ctggcgtcct cgatctcaca cccgtccacc atgcgatccc
     1681 ggcgtggaca cgcctggatc ccaaccgccc cggcgttcca atccagggcg cacgccctgg
     1741 cctccccccg gctccggtgg cgccgccatg gccagcccta ctccggccgt gggtgcggag
     1801 tcgaggtgcc ttcggcctgg cccatggccc agcgcatgcc cccgagcagg tgtgcctggt
     1861 agacgggact gtcgcagacc tcctcgcggt ggccgagagc cgtgtagaag ctgcgcccct
     1921 tgccgtagcg cttgcaccag gcgatgccga aatcaccgtc cttgcggtgg atccccggct
     1981 tcgtcatgtc ggtgcctgag gggtccagac tcatcagcac gcacacgtcg tctcgagacc
     2041 agtcgcgctg ctggtagatc tcgtcggcca cgacccagcc ttgcggaacg ggctcgcagg
     2101 cggggtggtc cggcacctcc acgaggagct tcacgtcctc gttccacggg tgtccgtcga
     2161 accaaccacc caccaggtcc cggtacgagg gccagtcgta gtgcgtgtcg gtggccgcat
     2221 ggatgccgac gaaggcgcca ccggccgaga cgaagtcgag cagcgcgacc ttctgcgagt
     2281 cattgagaaa tgacccggtg gtgttgccaa agatgacgca gtcgagccct gccagccgct
     2341 cctttgagat catccgggga tcctcggagc acaggaccga gaagccgttc tcctcgccca
     2401 agcgcgtgag gatgggtttc atgtacggcg cggcggagtg acggaagccc cgggtgcacg
     2461 agaagtagag cacggacttc tccggcgcca cggcgggcgc cgccttcgcc acgaccggct
     2521 cctcggcggc cccagcgggc gccgacgcct caggggcatc cggggagagc accgagagct
     2581 cgtggatcga ccagaaccag ctatccccat ggggcgccgt gagcgtgagc tgaaccgcgc
     2641 ggatcggagt cgggtcgaac tcgacccgga cgagcccgcc ccggttgatg gcgggcacat
     2701 cggcgatctc agccagcgac tgccacgtct tgccatcggt cgagccttcg accctcaagc
     2761 cgccgggata gtcctcggcc gacccacgcg cgtccagaat gacgcccgac acctgctgag
     2821 tcctgcccag gtcgagcagg taccagtcgc ccgacctctg cttggcgccc gtgtcccagc
     2881 gcgtctgcgg gttgccgtcg aaagccaggt cctcaccagc gccccgcgac gatttggccg
     2941 tccagccatc gacgggaagc ccccgtcgtg ggaccgatcg gaatgggcca taggccgatc
     3001 cggacagccg ggctcccccg gcatcgaaca gctccgtgac cacggtgagc gaggcgctgc
     3061 gccgctcgtc ccaccactcg cgacagtccg aaacctggac cgacccttcc gccgggaggt
     3121 cgacaatggc cgcctcggtg tagtggctgc catcagcgta gatcagcgtc cactcgactc
     3181 ggcagtccgg catggcgccg ggtgtgtcat tgctggccca gagggcggtg ggcgcctccg
     3241 tgccctccac gatcaccgag ggagcgccaa aggcggcacc cgacgcgacc gcgacaaccg
     3301 cgatacccac gacagcatgc gcgcgcatgg gacatcctcc gttcgcggga gcttcccgtc
     3361 ctaccgcggg gtgacctcaa gcagttcaac ctcaaacacg aggtcggcat tcggcggaat
     3421 gtcgggaggg gccccacgct cgccgtagcc cagcttcgcg gggatgatca gtttacgctt
     3481 cccgccctcc ttcatggtct ggagaccttc attccagccc tcgatgacct ggccgagcgt
     3541 gaaggtggcc ggttggcccc gctcgtacga agagtcgaac tccttgccgg tgtccttgag
     3601 tgtgccgcgg tagtcgacgg tcaccgagtc acccatcttg ggtgacttgc ccgtgcccac
     3661 cttgatgtcc tcatactgca gccccgacgc ggtcgtgact cgcttgccga tggccgatga
     3721 ctcgccctcg gctggcgcct gagccttggt atccgcctgg ccgctcggct cggtggtgcc
     3781 gggagc
//
LOCUS       DAOSPL010000260         3783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_24306_length_3783_cov_3.469957, whole genome shotgun sequence.
ACCESSION   DAOSPL010000260 DAOSPL010000000
VERSION     DAOSPL010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24306_length_3783_cov_3.469957"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLQ54_06115"
     CDS             complement(<1..488)
                     /locus_tag="PLQ54_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004052294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HOS92868.1"
                     /translation="MSAENHGTVPQNHVMEVTFESEGDRADPFNQVELDAIFTAPDGT
                     ERRVPGFWRGGRAWAMRYSSSIAGEHRFRTECSDVSDQGLHGQAGTVTVTPYDGDNPL
                     YRHGPITIAPDRRHFVHEDGTPFLWLGDTWWMGLCNRLHWPEDFHTLAADRVAKGFTV
                     IQ"
     gene            complement(634..3747)
                     /locus_tag="PLQ54_06120"
     CDS             complement(634..3747)
                     /locus_tag="PLQ54_06120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS92869.1"
                     /translation="MPDGPESTRGSSPREHIDVGLRAVLTAVDELAACPDLDTLYRRG
                     IELARERLGVERCSLFVCDGPCIRGTFGTDLHGQTTDERAYARNTTEYPDGRVVPSGP
                     DADRWIYWPSEVSEWEDGDFRAVRRGWVVCTPLWQGSELAGVFYNDTAITGAEVDSEL
                     QDRLAVYVSFLSSLIAQRQSERTQEELFRAALISVRELAECPNLDMLCRRAVELARER
                     LGVERCALYLVDGDVLRGTYGTDSEGRTTDEHALSRPLEEGSKAALSQAARSELPWTI
                     EQGVRLTALQGDDVVVVGHGWVAETVLAAHGEPIALFWNDAALSGAPPNQTRQWILSL
                     YCSVLAALIQRRRAEDTRWRMADGLERVIGAAAELMECPDPDTLYRRGVELARERLGI
                     ERCGLFLWDGERLHGTYGTDSSGRTTDERALVLERTGAWGRALHLRAPGERPYDLEEV
                     PLSHYDGKTQVMGPRGWVAMTTLATADRVIGGLANDAAISGSPPDEVQQQVLSVYCSI
                     LASLIELKRAEAERRDSQLRFQALIENSNDVIMILSAENTITYIAPTCRRILRWDPSE
                     VVGRHAREFIHPDDWPRVATALRRRLTAPGPGEYHEFRIRCGDGTWLDAEGLGYQPGP
                     GSPVDGTIVTVRDVAERKRLESHLRQTSKLEAVGTLVGGVAHDFNNLLTGILGFSDLL
                     LMNVTDTRHASQLRLIRDLAERGAGLVRQLLLFSRAQTGEQGPVDLNRVIEDLTRLLG
                     RVIGEDIHADLALAPDLVTVYADAAQMEQVLMNLAVNARDAMPNGGTLTIRTHNVKLH
                     REPPGALIRVSPGTYACVSVSDTGQGMDEATRERIFEPFFTTKEVGKGTGLGLATVYG
                     IVRQHQGTIEVDSRPGIGTTFRVYLPMGTGPASVTAERAATAAAPEGSETVLVVEDEP
                     SVLDLVCQVLEQQGYQVLPAATPSEAMSLFKAHGGHVDLLITDVVMPQMTGAALHQIL
                     VQEAPALRVLYMSGYPSHGGAGAAADLHGQPFLAKPFGPAILVARVREVLDGSGPSAQ
                     HGPCPYV"
BASE COUNT          610 a         1285 c         1258 g          630 t
ORIGIN      
        1 atctggatga ccgtgaaccc cttggccacg cggtcggcgg ctagtgtgtg gaagtcctcg
       61 ggccagtgca ggcggttgca gaggcccatc caccaggtgt cacccaacca aaggaacggc
      121 gttccgtcct catgaacgaa gtgccgccgg tctggcgcaa tggtgatggg gccatggcgg
      181 tacagggggt tgtctccgtc gtacggagta acggtgaccg ttccggcctg gccgtggagg
      241 ccctggtcgc tgacatcgga gcactccgtg cgaaaacgat gctcgccggc aattgatgag
      301 gagtaccgca tcgcccaggc tcggccgccg cgccagaaac cgggcacacg tcgctccgta
      361 ccatcgggcg ctgtgaagat ggcatcgagt tcgacctggt tgaacgggtc ggcgcgatcc
      421 ccttccgact cgaatgtcac ttccatgaca tggttctgcg gtaccgtccc gtggttctcc
      481 gcgctcatgg cgcccgctcc cccaatccag aaagccgtca gcatcgccag cggcctgacg
      541 tggcgtgcgg cggcaggttc tgaagccgtc agagtggagg tcagcatccg gtggtgcccg
      601 tcccgtccaa cacacgatgg gttccgggga cagctacaca tatgggcatg gcccatgctg
      661 ggcggacggc ccggacccat cgagtacctc gcgaactcgg gcgacgagga tggccggccc
      721 aaagggtttc gcgaggaacg gctggccgtg caagtccgcg gcggcaccag cgccaccatg
      781 gcttgggtag ccggacatgt agagcactct cagagccggc gcctcctgga cgagtatctg
      841 gtgcagggca gcaccggtca tctgtggcat gaccacgtcg gtgatcagca ggtcaacatg
      901 cccgccgtgg gccttgaaca gcgacatcgc ctccgaggga gtggctgcgg gaagcacctg
      961 gtagccctgc tgctcgagca cctggcatac caggtcgagt accgacggct cgtcttccac
     1021 cacgagaacc gtctcgcttc cctccggagc cgccgccgtg gcggcccgct ccgcagtgac
     1081 ggaggcaggg ccggtgccca tgggtaggta gacgcggaac gtggtgccga tccccggccg
     1141 cgagtccacc tcgatggtgc cctggtgctg gcgcacgatc ccatagacgg tggccagacc
     1201 cagtcccgtg cctttgccga cctctttggt tgtgaagaag ggctcgaaga ttcgctcgcg
     1261 cgtcgcctcg tccatcccct ggcccgtgtc cgagaccgag acgcaggcat aggttcctgg
     1321 gctcactcgg atcagcgcgc ccggaggttc gcggtgcagc ttgacgttgt gggttcggat
     1381 cgtgagtgtc ccgccgttgg gcatggcgtc tcgcgcgttg acggccaggt tcatgagcac
     1441 ctgttccatc tgggccgcgt cggcgtagac cgtgacgaga tcgggggcca gcgccaggtc
     1501 cgcatggatg tcttcaccaa tgacccgccc caggagccga gtgaggtcct cgatcacccg
     1561 attcaggtca accggacctt gctcgccggt ctgcgcgcgg ctgaacagca agagctgccg
     1621 gacaagccca gccccgcgtt cggccaggtc gcggatgaga cgcaactggc tcgcatgccg
     1681 tgtgtcggtc acgttcatga ggaggagatc ggagaacccc agtatgcccg tgagcaggtt
     1741 gttgaagtcg tgggcgacgc cgccgaccag ggtgccgacg gcctcgagct tcgaggtctg
     1801 ccgcagatgc gattcgagac gctttcgctc ggcaacgtcg cgcacggtaa cgatggtgcc
     1861 gtcgaccgga ctacccgggc cgggttggta gcccagccct tccgcatcca gccacgtgcc
     1921 atcgccgcac cggatgcgga actcgtggta ctcgccggga ccgggggcag tcagcctccg
     1981 ccgcagcgcc gtggccacac ggggccagtc atcggggtgg atgaactccc tggcgtggcg
     2041 cccgacgacc tcggatgggt cccatcgcag gatccggcgg caggtcggcg cgatgtacgt
     2101 aatcgtgttc tcggcgctga ggatcatgat gacgtcgttg gagttctcga tcagtgcctg
     2161 gaaccgcagc tggctgtcac gacgctcagc ctcggcacgc ttgagttcga tgaggctcgc
     2221 gaggatggag cagtagaccg acaggacctg ctgctgcacc tcgtcaggcg gcgatccgga
     2281 gatcgcggca tcgttggcga gccctccaat gacccgatcc gcagtcgcca gggtggtcat
     2341 cgcgacccag cctcgggggc ccatcacctg ggtcttcccg tcatagtggc tcagcggcac
     2401 ctcctcgagg tcgtagggcc gctcgcctgg tgcccggaga tgcagagccc tgccccaggc
     2461 tccggtccgc tcgaggacca aggcccgctc atcggtggtc cgcccgctgc tatcggtgcc
     2521 ataggttccg tgcagccgct ctccatccca gagaaacagg ccacagcgtt cgatgcccag
     2581 gcgctcccgc gcgagctcca cgccgcgccg atatagggtg tcggggtccg ggcactccat
     2641 gagctcggca gcggcgccga tgacccgttc gaggccgtca gccatccgcc accgggtgtc
     2701 ctccgcccgg cggcgctgga tgagagcggc caacacggag cagtacaggg acagtatcca
     2761 ctgccgcgtc tggttcgggg gggctcccga gagggccgcg tcgttccaga acagcgcgat
     2821 gggctcccca tgggctgcca gcaccgtctc ggccacccac ccgtgaccga caaccacgac
     2881 gtcgtcgccc tggagggcgg tgagcctcac gccctgctcg atcgtccagg gcagctcact
     2941 ccttgccgct tggctcaagg cggccttcga cccctcctcc aggggcctgg acagggcgtg
     3001 ctcatcggtc gtgcgcccct cggagtcagt cccgtaggtc ccgcggagga catcgccgtc
     3061 gacgaggtag agagcgcagc gctcgacccc gaggcgctcg cgcgcgagtt cgactgcgcg
     3121 gcggcagagc atgtcgagat tggggcactc cgcaagctca cgcacactga tcagcgcggc
     3181 cctgaacagc tcctcctggg tccgctctga ctggcgctgc gcgatgagcg acgagaggaa
     3241 cgacacgtac acggccagcc ggtcctggag ctccgagtcc acctcggcac cggtgatggc
     3301 cgtgtcgttg tagaacaccc ctgcaagctc tgagccctgc cagagggggg tgcacaccac
     3361 ccatcctctg cggacggccc ggaagtcgcc atcctcccac tcgcttacct ccgagggcca
     3421 gtagatccag cggtccgcat ccggtcccga cggtactacc cggccatccg ggtactcggt
     3481 cgtgttccgc gcataggccc tttcatcggt ggtctgtccg tgcaggtccg tgccaaacgt
     3541 gccgcggatg catgggccgt cgcagacgaa caacgagcac cgttccaccc cgagccgctc
     3601 gcgggcgagc tcgatccctc gccggtagag cgtgtccagg tcaggacagg cggccagttc
     3661 gtcgacagcc gtgagcaccg cccgcagccc gacgtcaata tgctcccgcg ggctcgaacc
     3721 ccgggtgctc tccggcccat ccggcatggg tgcccctcat cggcggcgac acgggttcgg
     3781 agc
//
LOCUS       DAOSPL010000261         3776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_24371_length_3776_cov_2.587208, whole genome shotgun sequence.
ACCESSION   DAOSPL010000261 DAOSPL010000000
VERSION     DAOSPL010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24371_length_3776_cov_2.587208"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PLQ54_06125"
     CDS             complement(<1..631)
                     /locus_tag="PLQ54_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92870.1"
                     /translation="MSIHTPSRIADRESIRGRANHAAPFWLATIAVLAIPWLIPLDYV
                     FVQPVVRLVYVATLAAGAVLFFFLPAQTPDRRWVLLFGLLTVVACTFAVTSFGSTGTV
                     LLVLRVLSGFALFRLVRNPTVVLALVVMFGTASLAVDYVLEDFAYLRLFDGLRFAPNT
                     EVSLRPRGFVGQAVPAGILAIVASVALAAAHSTQGRGAARAVVMIAAGVG"
     gene            complement(628..1824)
                     /locus_tag="PLQ54_06130"
     CDS             complement(628..1824)
                     /locus_tag="PLQ54_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92871.1"
                     /translation="MTGTALPVLMASGMIYVLPLMARPFLSASEYATWALGATVLSIT
                     LVFDLGSTAFVLTTAGTPLLTQKRLLASTGLAVAGALAPGLALAALWPWYSEGRGMQM
                     AGASGITYFLAITAAASLRSAALVSLAVMLATGRLRRRARALLGQAILQVVATASALY
                     LGAGIWALPIGVFLSGTALLVYVRSGFTAPVAVRAATQEVRLRSYARARLGLSALGLY
                     LTQLDRWVVGLIVPVTALAPYDTASRVAGLPRLVIIALSGVLVADSAHAHAAGVKAVA
                     LVYRKAFMATAGVGLALAVVVVMASSLVPVQSFAPVGLVAALTVAGTVNGITAPGTQI
                     LNGIGAPEVEYRYLLPCALSTTACWAIAAATANEAAALYVGAANLAVWSAYYVARMPS
                     ALRGAM"
     gene            complement(1905..2951)
                     /locus_tag="PLQ54_06135"
     CDS             complement(1905..2951)
                     /locus_tag="PLQ54_06135"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOS92872.1"
                     /translation="MTQILLLHAYSAANRGDGLLVDEALELLHQAFGDSCTVTLVARA
                     PETFAATGAKVLAAPPGPSFIRDLPQVLAAWRRADLVVGVGGGFARGDRALPFLKFFI
                     GHGWQLVLARARGRHAVYLPQSVGPFHPAIWPALRWAYGGLALAAVRDGRSLTDLGLQ
                     DTSRMPDCAVLTLDAAPTATAVDPVPVFTARPTGPRGDARCRALAARLGTFDSFVQSS
                     TLGNDDTSFVTSLGARAVVPAESLADPTAPRRVVVAVRLHAALMALRAGHLVIHLSYE
                     RKGFGAFEDLGLDDWVHNVNDVDVDLVANQVRALVEDTRARERYAAAVASALPAAVRH
                     RERLVHHLRGARRA"
     gene            complement(2948..>3776)
                     /locus_tag="PLQ54_06140"
     CDS             complement(2948..>3776)
                     /locus_tag="PLQ54_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOS92873.1"
                     /translation="TADTGPALGCRLPLVVTAHGVASRHTSVARTRTQETVWRARVRA
                     AVHQASLVLSVSHSAADDVAAVFGIDPASILVIPHGIDHLANHGGELSPRVERLLAST
                     EPFMLYLGNIEPRKNVDNLVRAVTEDGGVGMRLVVAGKPAWNFTPTLELMAQRPEVEY
                     LGFVTDADRAALLAGCRLFVFPSHYEGFGFPVLEAMSKGAAVICSNEGSLAEVAGPAL
                     RLPGLDPGSIREGLRTALSDGPTLSAVAAAGPAWAAQFTWEVSARRHAAAYESVLGS"
BASE COUNT          702 a         1205 c         1389 g          480 t
ORIGIN      
        1 ccccaacgcc tgccgcgatc atgacgacgg cgcgggcggc cccgcgccct tgcgtgctgt
       61 gtgccgcggc gagagccaca cttgccacga tcgcgagaat gccagcggga acggcttggc
      121 cgacgaaccc gcggggtcgc agggagactt cggtgttagg agcgaaccgt agcccgtcga
      181 acaaccggag gtaggcgaag tcctccagca cgtaatccac cgcgagggat gccgtgccga
      241 acatgacgac cagggcaagc accacggtcg gattccggac gagccggaag agcgcgaagc
      301 cggacagcac ccgcaggaca aggagcacgg tgccagtgga cccgaaggac gtgacggcga
      361 atgtgcaggc gacaacagtg agcaggccga acaggaggac ccaccgccgg tcgggcgtct
      421 gagcgggcag gaagaagaac aggacggcac ccgccgccag ggtcgccacg tagacgaggc
      481 gcaccaccgg ctggacgaag acgtagtcga gcggtatgag ccaggggatg gcgagcacag
      541 caatcgttgc gagccagaag ggggctgcgt ggttggcccg gccgcggatg ctctctcgat
      601 cggcgatccg gctgggtgtg tggatactca catcgcacct cgaagggctg atggcatccg
      661 ggccacgtag taggccgacc acacggcaag gtttgctgcg cccacgtaca gcgcggcagc
      721 ctcgttcgca gtggctgcgg caatggccca gcacgcggtt gtgctgaggg cgcagggcaa
      781 caggtagcgg tactccactt caggagcccc gatgccgttg aggatctgag tacccggggc
      841 ggtgatgccg ttcacggtgc cggcgacggt cagggcggcc acgagtccta ctggggcgaa
      901 agattgcacg gggaccagag acgacgccat caccaccacg acggcgagtg cgagaccgac
      961 ccccgcagtt gccatgaacg ccttgcgata gaccagcgcc acggccttca caccggccgc
     1021 gtgggcgtga gcgctgtcgg cgacgagcac tccgctcagc gcgatgatca ccaggcgtgg
     1081 cagcccggcg actcggctgg ccgtgtcgta cggcgccagc gccgtgaccg gcacgatcag
     1141 ccccacgacc catcggtcga gctgggtgag gtatagcccg agggccgaca gcccgagccg
     1201 ggctcgcgcg taggaccgca ggcgcacctc ttgcgttgcg gctcgcacgg ccacgggagc
     1261 ggtgaagccg gaccgcacgt agacaagcaa cgcggtgccc gagaggaata caccgatggg
     1321 caacgcccat attcccgcgc ctagatacag ggcggatgcc gtcgcgacga cctgcaagat
     1381 cgcctgcccg agcagggctc gggcacgccg gcgcaaccga ccggtcgcca gcatcacggc
     1441 gaggctaacc agcgcggcac tgcgaagcga ggcggctgcg gtgatcgcca ggaagtaggt
     1501 gatgcccgag gcgcccgcca tctgcatgcc acgcccttcg gagtaccagg gccagagagc
     1561 ggccaatgcc aatcctgggg ccagcgctcc ggcgactgcc agcccggtac ttgcgagcag
     1621 gcgcttctgg gtgagcaggg gcgtccccgc ggtggtcagg acaaaggcgg tggagccgag
     1681 gtcgaagacc agggtgatgg acagcaccgt ggcgccgagc gcccacgtgg cgtactcgga
     1741 ggcactgagg aacggccggg ccatgagggg aaggacgtag atcatgcctg acgccatgag
     1801 caccggtagc gcggtgcccg tcatgcgccg gagcccgccc gcaggtgtcc gcacccgcga
     1861 ggccgcggat gaaggggctg cgtcaccggg tctggaccgg gccatcacgc gcgcctcgcc
     1921 ccgcggaggt gatgaaccag acgttcgcgg tggcgaaccg ccgcaggcag tgcggacgca
     1981 acagccgcgg cgtatcgctc gcgggcccgc gtatcttcga ccagcgccct gacttggttg
     2041 gcgacgaggt cgacgtcgac gtcattgacg ttgtggaccc aatcgtcgag cccgaggtcc
     2101 tcgaaggcgc cgaagccctt gcgctcgtag gacaggtgga tgactaggtg accggcgcgc
     2161 agggccataa gggccgcgtg gaggcgcacc gctacgacaa ctcgtcgtgg cgcggtgggg
     2221 tcggcgagcg actcggcggg aacgacggcc cgggccccca acgacgtgac gaaggacgtg
     2281 tcgtcgttgc cgagggtcga gctctggacg aagctgtcga aggtgcccag ccgggccgct
     2341 agggcacggc accgggcgtc accccgaggg ccggtcgggc gcgcggtgaa gaccgggacc
     2401 gggtccacag ctgtggctgt cggcgcggca tccagggtga ggactgcgca gtccggcatc
     2461 cgcgatgtgt cttgcagccc gaggtccgtg agtgagcgtc cgtcgcgcac ggcggcgagg
     2521 gccagcccgc cataggccca gcgcagcgcg ggccagatcg cggggtggaa agggccgacg
     2581 gactggggca gatagacggc gtgacgacca cgagcgcgcg ccaagaccag ctgccatccg
     2641 tgcccgatga agaactttag gaacggcagg gcccggtcac ctcgggcgaa cccgccgccc
     2701 acgccgacga cgaggtcagc gcgacgccag gcggcgagga cctgcggcag gtcccggatg
     2761 aacgatggtc ctggaggggc tgcgagaacc ttggcgccag ttgcggcaaa ggtctcaggt
     2821 gcgcgcgcca cgagggtgac ggtgcaggag tccccgaacg cctggtgaag cagttccagc
     2881 gcttcgtcaa cgagaaggcc gtcgccgcgg ttcgcggcac tgtaggcatg cagcagcagg
     2941 atctgggtca cgaaccgagc accgactcat aggccgcagc gtggcggcgg gcgctgacct
     3001 cccacgtgaa ctgtgccgcc caagcgggtc cggccgcagc caccgccgac aatgtcgggc
     3061 cgtcgctgag cgcggtgcgc agcccttcgc gaatcgagcc cgggtcgagg cccggaagtc
     3121 gcagggccgg accggccacc tcggcgaggg acccttcgtt ggagcagatg acggcagcgc
     3181 ctttggacat cgcctcgaga acaggaaagc cgaagccctc gtagtggctt gggaacacga
     3241 agagacgaca gccggcgagt agggcggcgc ggtcggcatc cgtgacgaaa cccaggtact
     3301 cgacctcggg acgctgagcc atgagctcaa gcgtgggggt gaagttccaa gcaggtttac
     3361 cggccacgac gaggcgcata cccacaccac cgtcctcggt caccgctcgc acgaggttgt
     3421 cgacgttctt gcgcggttcg atgtttccca gatacagcat gaagggctca gttgaggcaa
     3481 gaagtcgctc gactctggga gagagctcgc caccgtggtt ggcgaggtgg tcgataccgt
     3541 gggggatcac gaggatactc gcagggtcga tgccgaagac tgcagctacg tcgtcagctg
     3601 ccgagtgcga cacggacagc accagcgagg cttggtgaac agcggcgcgc acccgcgctc
     3661 gccacaccgt ctcctgggtg cgggtgcgcg ccaccgaggt gtgccggctg gcgacaccgt
     3721 gagctgtgac cactaggggg agtcggcagc cgagggctgg gccggtatca gcggtg
//
LOCUS       DAOSPL010000262         3760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_24491_length_3760_cov_3.100405, whole genome shotgun sequence.
ACCESSION   DAOSPL010000262 DAOSPL010000000
VERSION     DAOSPL010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24491_length_3760_cov_3.100405"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PLQ54_06145"
     CDS             complement(<1..483)
                     /locus_tag="PLQ54_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92874.1"
                     /translation="MSHRFEFPLTALEGYGPQGVVPIRQRGTGRGRPRPPMATPVLQT
                     ALEMLEALEARGLGAAADARRRWFTRSGGANAKITFVSGNDVLLDVMGDGPSATVRLD
                     LGMLRTLAGHAGLGPLLAVGFASAAVYLRTASETDGLLAILDSYDRLAPEERSAVLDA
                     L"
     gene            complement(508..2106)
                     /locus_tag="PLQ54_06150"
     CDS             complement(508..2106)
                     /locus_tag="PLQ54_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOS92875.1"
                     /translation="MLRGIYGSDETLLEDRRQAWLAVLRRHARDGGTGAPALLARSPS
                     RVNVMGVHIDHRKGEVNYMTHRRETLMVATPRADDVVRLRNMRADTFPPREFSITEAL
                     GPRPWPDWVTLIESPHVADQVRESQGDWQNYVKAAVLRLQHFASDHPLHGLDLTVWGD
                     IPMAAGLSSSSALVVATAIAAVAANGLDIAPAHLVDLCGEGEWLVGTRGGAGDHSAMI
                     FGRRGLIAHVGFFPCRLLRYVPAPAGCDVVLCNSLRMAAKSAAELSAYNATIAAYGAA
                     LLVLKQVLVKDLGVDPSRLDDEIEHLGDFRLHARRYPDALLYRALKQIPERLSRAQLL
                     ERLPESREALERMFRTHDAPPEGYRVRAVAMFGLAEIARGAGCVEMLRHGDMRGFGEL
                     MYISHDGDRVVVHDDAGRVRPWDNERSRVSDAYLDGLVADLEGEDRACAMRAELMWQP
                     GGYGCSCEELDALVDICKRQPGVYGAGLTGAGLGGCVLALVAREQTPFLLAVLEKEYY
                     TARGLPMAAEPCVSVEGAGVVRTD"
     gene            complement(2260..3294)
                     /locus_tag="PLQ54_06155"
     CDS             complement(2260..3294)
                     /locus_tag="PLQ54_06155"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS92876.1"
                     /translation="MPRKLGCAIHGAGWVAGEHLKAYRANPHCEVVAVSSRRAEGARQ
                     CAAYAGCGEAAIYTSLDDLLADDRVDVVSICTPHDRHASETIQAARAGKHVLIEKPLC
                     TTVDDMRAMRDAVRAAGVKTLVSFCLRWNPLFHNIRCLLDQGAIGRVFYAEVDYWNGI
                     TPSYAGYSWIITRKQGGSALLAAGCHAVDALRYFVGSEAVEVSAYSVPATSDTPWEYD
                     PTMVLTVRFANGAVGKVSACLDTLIPYSFNINLLGSEGAIRNERVFSTRLFPQQTDYA
                     SVPTIQPTTRDVTHHPFVAEIAALVDAIRLDHRALPDLDDAVRTHEICFGAAQAAETG
                     KPVALPLLAD"
     gene            complement(3415..>3760)
                     /locus_tag="PLQ54_06160"
     CDS             complement(3415..>3760)
                     /locus_tag="PLQ54_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92877.1"
                     /translation="TVWMHSCGYVRDIIPDLVGIGVDVFQFDQPDLHTIDFLAQFADR
                     ATFWCPVDIQTTLQSGDEGTIKAKAREMVEKLGRGGGFIAKDYGDNFSIGVDPLWQHW
                     AYEAFVEAGTFR"
BASE COUNT          630 a         1293 c         1241 g          596 t
ORIGIN      
        1 cagagcatcg agcacggcgc ttcgctcctc gggggcaagc cgatcgtagg agtccagtat
       61 ggccaggagc ccatcagttt cggatgccgt ccgcaggtac acggctgcgg atgcgaagcc
      121 gacggccagc aggggcccca agccggcatg ccccgccaga gtccgcagca tgcccaggtc
      181 gagccgcacc gtggcgctgg ggccatcgcc catcacgtcg agcaggacgt cgtttcccga
      241 gacgaaggtg atcttcgcgt tggcaccacc ggaccgggtg aaccatcggc gccgggcatc
      301 ggccgcagct ccgagccctc gggcctcgag ggcctcgagc atctccagcg ctgtctgcag
      361 cacgggtgtg gccatggggg ggcgcggccg accgcgtccc gtacctctct ggcgtatggg
      421 gacaacgccc tgggggccgt agccctcgag cgcggtgagc ggaaactcga atcggtgcga
      481 cattcacaca gcctctctca cggtgcatca atcagtccgg accactccgg cgccttctac
      541 cgacacgcac ggctcggcgg ccatggggag tccacgggca gtgtagtact ccttctccag
      601 caccgccagg aggaacggcg tctgctcgcg cgcaaccagt gccagcacgc agccgcccag
      661 cccggcgccg gtgagcccgg ccccgtacac tcctggctgt cgcttgcaga tgtccaccag
      721 cgcgtccagc tcctcacagc tacagccgta gccacctggc tgccacatga gctctgcgcg
      781 catggcgcag gccctgtcct cgccctcgag gtcagccacg aggccatcga ggtacgcgtc
      841 ggacacgcgg gacctctcgt tatcccaagg ccgcacgcgt ccggcatcgt cgtgcaccac
      901 gacgcggtca ccgtcgtgcg agatgtacat gagctcaccg aaacctcgca tgtcgccgtg
      961 gcgcagcatc tcgacgcagc ccgccccccg ggcaatctcg gccagtccaa acatggcgac
     1021 ggcacgcact cggtatccct cgggcggcgc gtcgtgcgtg cggaacattc gctcgagcgc
     1081 ctcgcgggat tccggcagcc tctcgaggag ctgtgcgcgc gacagccgct cggggatctg
     1141 tttcagagcc cggtacagca gggcatccgg ataccgtcgt gcgtgcagcc ggaagtcgcc
     1201 caggtgctcg atctcgtcat cgagcctgga cggatcgacg cccaagtcct tcacgagcac
     1261 ctgcttgagg accagcagtg cggcaccata ggccgcgatg gtcgcattgt aggcactcaa
     1321 ttcggctgcg gacttggccg ccatgcgcag ggagttgcag aggaccacgt cgcacccggc
     1381 cggcgccggg acgtagcgca acagacgaca ggggaagaag cccacgtggg cgatcagacc
     1441 tcggcggccg aagatcatgg ccgagtggtc gcccgccccg ccacgcgtgc ccaccagcca
     1501 ctcgccctcg ccgcagaggt cgaccaggtg cgcgggcgcg atatcgagcc cgttggcggc
     1561 cactgcggct atggcagtcg ccacaaccag agccgacgac gaactcaggc ccgccgccat
     1621 gggtatgtcg ccccacaccg tgaggtcgag cccgtgcagc gggtgatcgc tggcgaagtg
     1681 ctgcagccgg agcacggccg ccttcacgta gttctgccag tccccttggc tctctcgcac
     1741 ctgatccgcc acgtgggggc tctcgatgag cgtgacccaa tcgggccacg gccgggggcc
     1801 cagggcttcg gtgatggaga actcgcgcgg ggggaatgtg tcggcgcgca tgttgcgcag
     1861 tcttaccacg tcatcggctc gaggcgtagc gaccatcagt gtctcgcgcc ggtgggtcat
     1921 atagttgacc tcgcccttgc ggtggtcgat gtggacgccc atgacgttca cgcgcgacgg
     1981 agaccgggcc agcagggctg gcgccccagt cccaccgtca cgggcgtggc gccgcaagac
     2041 cgccagccac gcctgccgac ggtcctccag cagggtctcg tcgctgccgt atatcccgcg
     2101 cagcactcgc ctccacgcac gggggtcctc aaaacgccgg atccactccc ccaccggcca
     2161 gacgcatgac atgcccggtc gcgggtatcc tggcagattc gtcggccgga ggcctcgggc
     2221 cattgggtcc tccccatcca tcgtagccgg gagcggcact cagtcggcga gcaggggcaa
     2281 ggcgaccggt ttccccgtct cggcggcctg cgcggcgccg aagcagatct cgtgcgtccg
     2341 gaccgcgtca tccaggtcgg gcagcgcccg gtggtccagc cggatggcgt caaccagagc
     2401 agcgatctcc gccacgaatg gatgatgggt gacgtcccgc gtcgtcggct ggatggtcgg
     2461 tacggatgcg tagtccgtct gctgcgggaa cagccgggtt gagaagaccc gttcgttgcg
     2521 gatggcaccc tcggaaccca gcaggttgat gttgaacgag tatgggatca gcgtgtcgag
     2581 acatgccgag actttgccca cggccccgtt cgcgaagcgc acggtcaaca ccatggtcgg
     2641 gtcgtactcc cagggcgtgt cgctggtggc cggcaccgaa tacgccgaca cctcaacggc
     2701 ctcgctgccc acaaagtacc gcagcgcgtc gaccgcgtgg catccggcgg cgagcagggc
     2761 gctcccgccc tgcttgcgcg tgatgatcca cgaatagccc gcgtacgacg gcgtgatccc
     2821 gttccagtag tccacctcgg cgtagaagac gcgaccaatg gccccttggt ccagcaggca
     2881 ccggatgttg tgaaacagcg ggttccagcg gaggcagaag ctgaccaggg tcttcacgcc
     2941 ggccgcgcgc accgcatcgc gcatggcccg catgtcatcg accgtcgtgc acaacggctt
     3001 ctcgatcagc acatgcttgc ctgccctcgc agcctggatg gtctcggacg cgtggcgatc
     3061 gtgaggtgtg cagatggaga ccacatcgac gcgatcgtcc gccagtaggt cgtccagtga
     3121 tgtgtagatc gcggcttcgc cacagccggc gtaggcggcg cattgacggg ccccctcggc
     3181 cctgcggctg ctgaccgcca cgacctcgca gtgcgggttg gcgcggtaag ccttcaggtg
     3241 ctcgccagcc acccagccgg cgccatgaat cgcgcacccc agctttctgg gcatcgagat
     3301 gatcctccgg tgtagagacg tcttggatca cggggttcgg caagtgaggc caggaggcct
     3361 gcggacaggc gaggccggca aacgtgcgcg catggtgggc aacgtcagag atcgctaccg
     3421 gaacgttccc gcctcgacga acgcctcgta ggcccagtgc tgccacaggg gatcgactcc
     3481 gatggagaag ttgtccccgt agtccttggc gatgaacccg ccgcctcggc cgagcttctc
     3541 gaccatctcc cgtgctttag ccttgatggt gccctcgtcg ccgctctgca gtgtggtctg
     3601 gatgtccacg gggcaccaga aggttgcccg gtcggcgaac tgggccagga agtcgatggt
     3661 gtgtagatcg ggctggtcga actggaacac gtccacgcca ataccgacga ggtcggggat
     3721 gatatcgcgc acgtacccgc acgagtgcat ccacacggtg
//
LOCUS       DAOSPL010000263         3757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_24521_length_3757_cov_2.939762, whole genome shotgun sequence.
ACCESSION   DAOSPL010000263 DAOSPL010000000
VERSION     DAOSPL010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24521_length_3757_cov_2.939762"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2945)
                     /locus_tag="PLQ54_06165"
     CDS             complement(<1..2945)
                     /locus_tag="PLQ54_06165"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOS92878.1"
                     /translation="MPHTRACWIWVVALCAAAAGEGGLTALPYVGPDPAEWTTALDAA
                     RLAEELVAFGVEAVDDDAEGRVLRWSFEMRPAAPRFNDLFCMEPIERRFAALRIHLRN
                     AGAGFALAVKLLEADGSEYTARRVALDTNGDWTDVEWPWTEFVVAPWSRDDNDTLNPP
                     LRGLALIAFDVSPGKQYDLRVTGVELMRETPPIVDLKASGVPERVRAGDEFGMDLSLT
                     PRSPITEDAACAVELARHGRPVWSHPVRLLRPTSEWRPGEVIELPRQTLRVPLYLWGG
                     EYDVRVVLGDCHVRREGHPAGQVATLEVQQRHPRPCQAQVRPHNGVPTLFINGRPNSA
                     MSYMTYQPTPRYFGQFGEAGVHVYSFPSTCTRHTWQSFARQAWLAPGDHFDFSQLDEQ
                     VLRILEADPEAYVFPRVYVNSPDWWDKEHPDDLVMYDDGTGEPKLFLEAEGKPCPSWA
                     SQAWRDDAGYALRRYIQHVRSSPYADRVLGYHVASGTTEEWMYWGANEGRFCDYSPVA
                     RQAFRRWLRDRYGSDAALQAAWGRADVTLDTADVPSFARRSATEHFAFRDPTAARDVI
                     DLHLFLANITTEAIRALARVVKDETDGQALMGTFYGYVLQLVGEQRQQNAGHLDLMGV
                     LDCPDIDFVTSPTSYAFRTLGDGYSHFMSLLDSVKLHGKLWFDENDIRTWLVDAPAGQ
                     WGRYDTFEDSLRMQQREFANVIGNGCGMWWFDMGGGWYDDERMLAAIERMRAIADETV
                     HWDRSPVAEVAMVVDDASLAAMQTGNAISWPLLMQQMLELGRIGAPIATYALADIGAM
                     PDMKLYLFANAFAPTEDQVEAIRRKVRRNGAWAVWIYAPGVVDGGQLRPEAAERLTGF
                     RLGMVDEQAPLTVSLTADGHAATGLRDMTYGPATAFGPVFFADDPAIEAWGTLGANAK
                     CGLAARRFHDWTSVYSAAPALPTALLRRLAAAAGVHLYADAGDAVYANRSVLAVAFGS
                     PGEKVIRLP"
     regulatory      3171..3299
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3453..>3757
                     /locus_tag="PLQ54_06170"
     CDS             3453..>3757
                     /locus_tag="PLQ54_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS92879.1"
                     /translation="MLRRVTDRQRGFTLIELLVVIAIIAILAAILFPVFAKAREKARQ
                     ASCASNVRQLALAWQMYAQDYDETACLSYYYSADFMVEYAWDFIVDYGAGSANLGLLG
                     "
BASE COUNT          623 a         1275 c         1258 g          601 t
ORIGIN      
        1 tggggcaggc ggatgacctt ctcgcccggc gaaccaaagg cgacggcgag cacactgcgg
       61 ttggcgtaga cggcatcgcc ggcgtcggcg tagaggtgaa ctcccgcagc cgcggccagt
      121 cgtcgcagca gggcggtcgg tagcgccggc gccgccgagt agaccgacgt ccagtcatgg
      181 aaacgccgcg cggccagccc acacttggcg ttcgcgccga gcgtgcccca agcttcgatg
      241 gcgggatcgt cggcgaagaa cactgggccg aaggccgtgg cgggcccgta cgtcatatcg
      301 cgcagcccgg tcgccgcgtg gccatcggcc gtcaggctca ccgtcagcgg ggcctgctca
      361 tcgaccatgc ccagccggaa tcccgtcagg cgttcagccg cctccggccg gagctggccc
      421 ccgtcgacca cgcccggcgc atagatccac accgcccacg cgccgttacg cctgactttc
      481 ctgcggatgg cctcgacctg gtcctccgtg ggcgcgaatg cgttggcaaa gaggtacagc
      541 ttcatgtccg gcatggcccc gatgtcggcg agtgcgtacg tggcgatggg cgcgccgatg
      601 cggccgagct ccagcatctg ctgcatcaac agcggccacg agatcgcgtt gccggtctgc
      661 atggcggcca ggctggcatc gtcgaccacc atagccactt cggcgaccgg cgaacggtcc
      721 cagtggacgg tctcgtcggc gatggcgcgc atccgctcga tggcggccag catgcgctcg
      781 tcgtcatacc aaccacctcc catgtcgaac caccacatgc cacatccgtt gccgatgacg
      841 ttcgcgaact cgcgctgctg catgcggagc gagtcctcga acgtgtcata gcgcccccac
      901 tggccggccg gcgcgtcgac cagccacgtc cggatgtcgt tctcgtcgaa ccacagcttg
      961 ccgtgcagct tcaccgagtc cagcagcgac atgaagtggc tatagccgtc gcccagcgtg
     1021 cggaatgcgt aactggtggg gctggtcacg aagtcgatat ccgggcagtc gaggacgccc
     1081 atgagatcga ggtggcccgc gttctgttgg cgctgctcgc cgacgagctg gagcacgtag
     1141 ccgtagaagg tgcccatcag cgcctggccg tccgtctcgt ccttgacgac gcgcgccagt
     1201 gcacgaatgg cctctgtcgt gatgttcgcc aggaaaagat ggaggtcgat gacatccctc
     1261 gccgccgtgg ggtcacggaa ggcgaagtgc tcggtggccg accgccgcgc gaaggacggc
     1321 acgtcggccg tgtcgagcgt tacgtcggcc cgtccccatg cggcctggag ggcggcatcg
     1381 gagccgtagc ggtcccggag ccaccggcga aaggcctgcc gggccacggg gctgtagtca
     1441 cagaagcgtc cctcgttggc accccagtac atccactcct cggttgtgcc actcgcgaca
     1501 tggtagccga ggactcggtc ggcatagggg gagctccgca cgtgctggat gtagcgccgc
     1561 aaggcgtagc ccgcgtcatc gcgccatgcc tgggaggccc acgaggggca tggcttgcct
     1621 tcggcttcga ggaagagctt cggttctccg gtgccgtcgt catacatgac caggtcgtcg
     1681 gggtgctcct tgtcccacca gtcgggcgag ttcacgtaga cccgcgggaa cacgtacgcc
     1741 tccgggtcgg cctcgaggat ccgcagaacc tgctcgtcga gttgggagaa gtcgaagtgg
     1801 tcgccgggcg cgagccacgc ctggcgcgcg aagctctgcc aggtgtggcg cgtacacgtc
     1861 gacgggaagg agtagacgtg caccccggct tcgccgaact ggccgaagta cctgggcgtc
     1921 ggctggtagg tcatgtacga catggcggag ttcggacggc cattgatgaa cagcgtgggc
     1981 acgccgttgt gcggcctcac ctgggcttgg catggcctcg ggtgacgctg ctggacttcc
     2041 agtgtggcga cttggcccgc cggatgcccc tcccgccgca catgacagtc gcccagcacc
     2101 actcgaacgt cgtactcgcc cccccacagg tacaacggca cccggagcgt ctggcgcggt
     2161 agttcgatga cctcgccggg tctccactcg ctcgtggggc gtaacagacg caccgggtgg
     2221 gaccagaccg ggcgaccatg gcgcgcgagc tcgacggcgc aagcggcgtc ctcggtgatg
     2281 ggcgaccggg gagtcaacga tagatccata ccgaactcat cgcccgcacg gacccgctcc
     2341 ggcacgcctg aggccttcag atccacgatt ggcggcgtct cgcgcatcag ctccactccc
     2401 gtcacgcgca ggtcatactg cttgccaggg ctcacgtcga aggcgatcag cgccagtcca
     2461 cgcaatggtg ggttgagcgt gtcgttatca tcccgggacc atggagccac aacaaactcc
     2521 gtccacggcc attcgacgtc ggtccagtcg ccgttcgtgt cgagggcgac gcgccgggcg
     2581 gtgtactcgg agccatcggc ttcgagaagc ttcacggcca gcgcgaaccc ggcgccggca
     2641 ttgcggaggt ggatcctcag ggcggcgaag cgccgctcga tgggctccat acagaacagg
     2701 tcgttgaacc ggggtgccgc gggtcgcatc tcgaagctcc agcggaggac gcgcccctca
     2761 gcatcatcat caacggcctc cacgccgaag gccaccagct cctcggccag acgcgcggcg
     2821 tcgagggccg tagtccattc cgccgggtca gggccgacgt acggtagggc agtgagaccg
     2881 ccctcccccg cggcggccgc gcagagcgcc accacccaga tccagcaggc gcgagtgtgc
     2941 ggcacggaac ccactccccc cggggccaga taacgaccag agggtggttc gccgagtggg
     3001 cggcgatgac ccttgcccgg agacgaggcg gtacccagac cacgccagtg gcgccgtcat
     3061 cacctgctca ccccttgttg cacctgcact caccgagtac aatcctggca tagctgaatg
     3121 cacttgagcc ggcgtggcac ggtggtgcgg ccctgcgggt tgcgccatcc ggaagacggt
     3181 gcgatgccgt cgctgccccg caactgtaac cggggacgaa cccccgcacg gaacgccact
     3241 gggaccgttg gtggtcctgg gaaggcgcgg ggagtagggc gatccgggag ccaggagacg
     3301 accacgccgg cggtcagcca gcgctccttc gtgggaaggg gagcggccgt ttgtttggct
     3361 gccacatccg cgttactcag aggggccacc cgttgcggtc ggggcacgca acgggtgcgc
     3421 ccctcttgtc gtctccgaga ggatgggctt ccgtgctccg acgcgtcacc gaccgccaaa
     3481 gaggcttcac gctcatcgaa ctactcgtcg tcatcgccat catcgcgatc ctggccgcga
     3541 tcctgttccc cgttttcgcc aaagctcggg agaaggcgcg gcaggccagc tgtgcctcga
     3601 acgtccgtca gctcgcactc gcctggcaga tgtacgccca agactatgac gagaccgcct
     3661 gcctctcgta ctactactcg gccgacttca tggtcgagta tgcgtgggac ttcatcgtcg
     3721 attacggcgc gggcagcgcg aacctgggcc tattggg
//
LOCUS       DAOSPL010000264         3754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_24548_length_3754_cov_2.751014, whole genome shotgun sequence.
ACCESSION   DAOSPL010000264 DAOSPL010000000
VERSION     DAOSPL010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24548_length_3754_cov_2.751014"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PLQ54_06175"
     CDS             complement(<1..789)
                     /locus_tag="PLQ54_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOS92880.1"
                     /translation="MPDEEREARRALACVVEAGEPRLASALGKAEPQVIWERVAAMAG
                     RRLEDGSHDPWVRRASAIDLDAVRAGEVAHGLRFIIPGDDEWPAGLSALEGCPAVQGI
                     AGAPIGLWVRGPRPLAALLEVSVSIVGSRAATSYGERVASSLAGGLAEAGVGVISGGA
                     YGIDAAAHRGALGEKGTTVAMLACGLDDPYPKLNVGLLESVARAGALVSEYPPGAHPT
                     RPRFLARNRLIAALSQGTVLVEAAARSGARNTVSWATGCGRPVMA"
     gene            complement(782..2323)
                     /locus_tag="PLQ54_06180"
     CDS             complement(782..2323)
                     /locus_tag="PLQ54_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOS92881.1"
                     /translation="MRLARAWSIALVGMEATLVEVEVSIGGGLPNTMIVGLGDTAVKE
                     ASQRCRSAVAAAGFAWPPSSVTVNLTPADMPKSGSHYDLPLVAATMAASGVVPRRALE
                     ATLFLGELGLDGRVRSVRGVLPAVLGASRSGFERAVVPLGQIREAQLVEGIGVVGVES
                     LGHLVAVLKGEPYDVDVVDPAAGSDEVAGSKDLADVVGQPEARWALEVAAAGGHHVSM
                     VGPPGVGKTLLAERLPGILPDLAVDEALEVSAIHSMLGASLEGGLIRRPPYADPHHSA
                     SLPALVGGGPHLARPGAVSRAHRGVLFLDEASEFSPAALEALRTPLEKGVIVLARSQG
                     AATYPARFQLVLASNPCPCGWYNVAGFASRCRCQPYAIKRYSSRLSGPIIDRIDIQLR
                     LQPLSKSHLRGNHPTEETSAVVAQRVLEARSRQRHRLDRHGFSRNAEVPGSLLHRDLP
                     NPTGVEDVDAAVRRGVLSSRGVDKVLRLAWTIADLMGHGVPDRGDVHTALAMRQGETE
                     EVNCA"
     gene            complement(2320..2697)
                     /locus_tag="PLQ54_06185"
     CDS             complement(2320..2697)
                     /locus_tag="PLQ54_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOS92882.1"
                     /translation="MTHGVSDRRETGVSGEDIAAVHLARRGWLIVARNWRCRSGEIDI
                     IARDPDGVLVVVEVKTRRGLGFGDPLESLTYAKVRRLKGLAMEWVRSQEDAVGPVRLD
                     AIGVLLDADAAPRLRHVRNLEQP"
     gene            complement(2783..3097)
                     /locus_tag="PLQ54_06190"
     CDS             complement(2783..3097)
                     /locus_tag="PLQ54_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HOS92883.1"
                     /translation="MSAEDLEQYESELELQLYREYKDVVGIFTYAVETERRFYLCNAV
                     DLKVRTEGGDVYYEVSMGDAWVWDMYRPARFVKSAKVLTFRDVSIEEIAHADLKIPDD
                     LR"
     gene            complement(3094..3732)
                     /locus_tag="PLQ54_06195"
     CDS             complement(3094..3732)
                     /locus_tag="PLQ54_06195"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOS92884.1"
                     /translation="MAFVIRRAAGLYGYERALARAGFVRVAGADEAGRGACAGPLVAG
                     ACILGSRAIEGLDDSKKLTAPARERLYDIITKRAVAWSVVSISPAECDALGMHVANLE
                     ALRRAVWRLGVQPDFVLTDGFSVDGLGQPSLAMWKGDAVAASVAAASILAKVTRDRIM
                     ADYHADYPDYRFDVHKGYCTKLHQDALDAVGPCAIHRLKWDNVARTIRLGEL"
BASE COUNT          611 a         1443 c         1184 g          516 t
ORIGIN      
        1 ggccatcacg gggcgaccgc acccggtcgc ccagctcacg gtgttgcggg cgccgctcct
       61 ggcggcggcc tcgacgagca ccgtgccctg ggatagagcc gcgatcaggc gattgcgggc
      121 taggaaccgg ggtctcgtgg gatgtgctcc gggcgggtac tcggacacca acgcccccgc
      181 ccgcgccacc gactccagga gtcccacgtt gagcttcgga tacggatcat cgagcccgca
      241 ggccagcatc gcgaccgtcg tccccttctc ccccagtgcg ccccggtgcg cggcggcatc
      301 gatgccgtac gcgccgccgg agatcacgcc gacaccggct tcggcgagcc ctcccgccag
      361 cgaggacgcg acccgctccc cgtaggacgt cgcagcccgc gaccccacga tggacactga
      421 cacctccaac aacgccgcca gcggacgggg accacgcacc cacagaccga tcggcgcccc
      481 tgcgatgccc tgaaccgcgg ggcacccctc gagagcggac agaccagccg gccactcgtc
      541 gtcgccaggg atgatgaacc gcagcccatg ggccacctca cctgcacgga ccgcgtccag
      601 atcgatcgcg gacgcccggc gcacccaagg gtcgtggctc ccatcctcca gccgtctgcc
      661 cgccatggcc gcgacgcgct cccagatcac ctgcggctcc gccttgccca gcgcggacgc
      721 cagccgcggc tcacccgcct ccaccacgca ggcgagagcc ctgcgtgcct ctcgctcttc
      781 gtcaggcaca gttcacctcc tcggtctcgc cctgtctcat ggccagcgcg gtgtgtacgt
      841 cgccccgatc gggaaccccg tgccccatga gatcggcgat cgtccaggcc agccggagca
      901 ccttgtcgac gcctctcgaa ctgagcacgc cccgccgaac ggcggcgtcg acgtcctcca
      961 caccggtcgg gttgggcagg tcccgatgaa gcagagagcc ggggacctcc gcgttgcgcg
     1021 agaacccgtg ccggtcgagg cggtgtcgct gccgcgacct ggcctccagc actcgctgcg
     1081 ccacgacggc agaggtctct tcggtggggt ggttgccccg caggtgagac ttgctcaagg
     1141 gctgcagccg cagctggatg tcgatccgat cgatgatcgg cccggagagt cggctcgagt
     1201 accgcttgat cgcgtacggc tggcaccggc accgggacgc gaatcccgcc acgttgtacc
     1261 agccacacgg gcacgggttg gaggccaaca ccagctggaa ccgggcgggg tacgtggctg
     1321 ccccctggga ccgcgccagc acgatcaccc ccttctccaa cggcgtccgc agcgcctcca
     1381 gggccgccgg cgagaactct gacgcctcat ccaggaacag gacgcctcgg tgggccctcg
     1441 acaccgctcc cggacgtgcc aggtgcggac ccccgcccac cagcgcgggg agggacgccg
     1501 agtggtgggg gtcggcatag gggggccgcc gaatgagtcc gccctcgagg gacgcaccca
     1561 gcatcgagtg gatcgccgac acttcgagag cctcgtccac ggccagatcg ggaaggatgc
     1621 ccggcagccg ctcggccagc agcgtcttgc ccaccccagg agggccgacc atcgacacgt
     1681 gatggccccc tgccgcagcg acctcgagcg cccaccgcgc ctcgggctga ccgacgacgt
     1741 cggccaggtc tttgctcccc gccacctcgt cactgccagc ggcaggatcg accacgtcca
     1801 cgtcataggg ctcgcccttc agaaccgcca cgaggtgccc cagcgactcc acacccacca
     1861 cgccgatgcc ctcgacgagc tgcgcctcac ggatctggcc cagcggcacc accgcccgct
     1921 cgaacccgga ccggctcgcg cccagcaccg ccggcagcac cccgcggacc gatcgcaccc
     1981 gcccgtccag acccagctcg cccaggaaca gcgtcgcctc cagcgcgcgc cggggcacca
     2041 caccggacgc ggccatcgtg gccgccacca ggggcaggtc gtaatgcgat ccgctcttgg
     2101 gcatgtccgc gggggtgagg ttgacggtga ccgacgacgg cggccaggcg aaaccggccg
     2161 ctgcgaccgc ggaccgacac cgctggctcg cttccttcac ggccgtgtca cccagcccga
     2221 cgatcatcgt gttgggcagg ccgccaccga tcgacacctc gacttcgacc agtgtcgcct
     2281 ccatgccgac cagcgcgatc gaccacgccc gcgcgagcct cacggctgct ccaggttgcg
     2341 gacgtgccgc agccggggag ccgcgtcggc gtccaacaag accccgatgg cgtccaggcg
     2401 aaccgggccg acggcgtcct cctgcgatcg gacccactcc atggccaggc ccttgagtcg
     2461 gcggaccttg gcgtaggtca acgactccaa cgggtcgccg aagccgagcc cgcgccgcgt
     2521 cttgacctcg acgacgacca gcacgccgtc gggatccctc gcgatgatgt cgatctcgcc
     2581 ggacctgcac cgccagttgc gggccacgat cagccagccg cgacgcgcca ggtgcacggc
     2641 cgcgatgtcc tcgcccgaaa cgccggtctc gcgccggtca ctcaccccat gtgtcatctg
     2701 ccacctccgc gcacacattg ggcgcgggag ggcgaatatt tgcagcctgt ggataactct
     2761 tgttcagtta cccggctcaa gctcagcgga ggtcgtcggg aatcttcaga tccgcgtgcg
     2821 cgatctcctc gatcgagacg tcgcggaagg tcaacacctt cgccgacttc acgaagcgcg
     2881 ccggacggta catgtcccag acccaggcat cccccatgga cacctcgtag tagacgtcac
     2941 cgccttcggt gcgaaccttg aggtcgaccg cgttgcataa gtagaagcgc cgctcggtct
     3001 cgacggcgta ggtgaagatg ccgacgacgt ccttgtactc gcggtacagc tggagttcca
     3061 gctcgctctc gtactgctca agatcctcgg cgctcataac tcccccagcc taatagtccg
     3121 agccacgttg tcccacttca ggcggtggat cgcgcacggc cccaccgcat ccagcgcgtc
     3181 ctggtgcagc ttggtgcagt agcccttgtg gacgtcgaac cggtaatcgg ggtagtcagc
     3241 gtgataatca gccatgatcc ggtctcgggt caccttcgcc agaatggacg ccgccgccac
     3301 ggacgccgcc acggcgtccc ccttccacat cgccagcgaa ggctgcccca gaccgtcgac
     3361 gctgaagccg tcggtgagca caaaatccgg ttggacgccg aggcgccaga ccgctcggcg
     3421 cagggcttcc aggttcgcga cgtgcatgcc gagcgcgtcg cattccgctg gggagatgct
     3481 caccacggac caggccacgg cgcgcttcgt gatgatgtcg tacagccgct cgcgggcggg
     3541 cgccgtgagc ttcttggagt cgtccaagcc ctcgatagcc cggctgccca ggatgcacgc
     3601 gccggccacc agcgggccag cgcaggcccc gcgtccggcc tcgtcggccc ccgcgacgcg
     3661 gacgaacccg gcccgggcga gcgcacgctc gtagccgtac agaccggcag cccgccggat
     3721 cacgaacgcc atggcccccc taacaggcgg gcgg
//
LOCUS       DAOSPL010000265         3751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_24579_length_3751_cov_2.780844, whole genome shotgun sequence.
ACCESSION   DAOSPL010000265 DAOSPL010000000
VERSION     DAOSPL010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24579_length_3751_cov_2.780844"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PLQ54_06200"
     CDS             <1..724
                     /locus_tag="PLQ54_06200"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOS92885.1"
                     /translation="GATPAHMVSDLLSVSLPAANLAKADAMGLDVLLPCAACYNRLAV
                     ARERLHAEPTLRAQVEQALGQPVAAAQKTLHPLSVIDSAEVARRVTRPLSRVRAVAYY
                     GCLLTRPASVSIDANVVNPTVMDELMSACGVQMLDWPLKTECCGATLTMGRREAAVRL
                     TARILQCAQAVGADCVVTACPLCQSNLDVRQDQVARLLGTPMNMPVVFFTEVLALALG
                     VDAARLDLDRHIVSAVHLAAAR"
     gene            808..2871
                     /locus_tag="PLQ54_06205"
     CDS             808..2871
                     /locus_tag="PLQ54_06205"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOS92886.1"
                     /translation="MMGLIGAIPRVRYAGWVLVGVAVMVFEANAHVGPREEELATANR
                     WLGEHLLQPEASLPFSFVYGGRGSATLLRDWPVERETARLDAVRTRHVLRWTDPASEL
                     QVRCESIEYADYPAVEWTVYLRNTGTQNSAVLERIQGLDTRFDRGAEGEFTLHGIKGD
                     SCSADSFEPYARVLAPNTEARFGPPGSGKSSDGPDGWPYYNLQFTGGGTIIAVGWPGQ
                     WASSFVRDEATGLGVRAGQEFTRLYLRPGEEVRTPLIALLFWSGDDVVRSQNLWRRWY
                     VAHNMPRVDGRTQRAVAQIQVGGSEADIAYVQSFLDAGIRVDLCWRDAGGSRDSVWFP
                     VGDGPFHEPDPAWLNSGTWDVDLTKYPRGFRPFTEWIHAHGMEFVLWFEPERVGDPDS
                     WLGRNHPEWLLPNNWGGAILNEGDPAARQWLIDHIGDIIAREGVDWYREDMNGGGPAP
                     AWRAHDAPDRQGITENLYVQGHLAYWDALRRRHPRLRIDSCASGGRRNDLETMRRAVP
                     LLRSDFQWPEMGDVAEGNQGHTYGLSAWLPFQGTGVYQYDPYSYRSFYLPSFGMGVLT
                     PENREAQSRAYSECRQVGPRMLGDYYPLTPYSRDLDRWMAWQFDRPNEGDGVVQAFRR
                     RQCAEDVINVRLSGLDPYARYGLTDLDRAGTAEASGRELMTEGLRVEVSEKPGAAIIL
                     YSKVR"
     gene            complement(2968..3378)
                     /locus_tag="PLQ54_06210"
     CDS             complement(2968..3378)
                     /locus_tag="PLQ54_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HOS92887.1"
                     /translation="MRRILTVAMAGVLVAGGLMAWMARADEFTTEDQERWQGGFMSVV
                     KEGRALWTSGELGTNGVACAQCHPNAANPPPETYPKFQQQLGRVVPLRDMINWCLQHP
                     LEGQALALDDPKMIALESYITYERRGVKLAPGKH"
     gene            complement(3394..>3751)
                     /locus_tag="PLQ54_06215"
     CDS             complement(3394..>3751)
                     /locus_tag="PLQ54_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013817673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HOS92888.1"
                     /translation="RKPTVVYGHVHQIQYNQIGNIAFQAVMATAWPWPYPETYAQAEP
                     DFPPITVPMNRANPFFERDATGWQGIDVEAGRVTLNYRLFNNTDRTIGFDPAKKRPVD
                     RDFPDKDKRIPPQDHF"
BASE COUNT          560 a         1173 c         1335 g          683 t
ORIGIN      
        1 tggcgccact ccggcgcaca tggtgagtga cctgctgtcg gtctcgctgc ccgcggcgaa
       61 cctggcgaag gccgacgcaa tggggcttga cgtgttgctc ccgtgtgcgg cgtgctacaa
      121 ccgtctggcg gtggcgcggg agcggctcca cgccgagccg actctgaggg ctcaagtcga
      181 gcaggctctc ggtcagccgg tggccgcagc ccagaagacg ctgcacccgc tgagtgtcat
      241 cgactcggct gaagttgcgc gccgggtgac gcggccgctg tcgcgggtcc gggcggtggc
      301 ttactacggc tgtctgctga cgcggcccgc ctcggtatcg atcgatgcca atgtggtcaa
      361 tccgacggtg atggatgagt tgatgtcggc ctgcggcgtg cagatgctcg actggccgct
      421 gaagaccgaa tgctgcggcg cgacgctgac catggggcgt cgtgaggccg cggtgcggct
      481 caccgcccgc atccttcagt gcgcccaggc cgtgggcgcc gactgcgtgg tcactgcatg
      541 ccctctgtgt cagtccaacc tcgatgtgcg ccaggaccaa gtggcccggt tgctcggcac
      601 cccgatgaac atgccggtgg tgttcttcac ggaagtgctc gccctggcgt tgggcgtcga
      661 cgcggcgagg ctcgacctgg atcggcacat cgttagcgcg gtgcacctgg ccgcggcgcg
      721 gtaggtcccg gggccgcgcc acgcggcgcc acacttggta cgtggccgcc atggctcctg
      781 gaccaagctc gaggaggggc tctgcgcatg atgggactga tcggagccat accgcgtgtg
      841 cgctatgcgg gctgggtgct ggtgggggtg gcggtgatgg tgttcgaagc gaacgcgcac
      901 gtggggccga gggaagagga gctggccacc gcgaaccgct ggctcggtga gcacctcctg
      961 cagcccgagg catcgctccc cttctctttc gtctacggag gccgggggtc ggcgacgctg
     1021 ctgcgggact ggccggtcga acgggagacg gcacggctcg atgcggttcg cacgcgccat
     1081 gtgctccggt ggacggatcc cgcgtcggag cttcaggtgc gctgcgagtc gatcgagtat
     1141 gccgactatc cggccgtcga gtggaccgtc tacctccgga acaccgggac ccagaactca
     1201 gccgtcctcg agcgcatcca gggcctggac acgcgctttg accgaggcgc ggagggtgag
     1261 ttcaccctcc atggcatcaa gggcgactcg tgcagtgccg atagctttga accctatgcg
     1321 cgggttctag cgcccaacac cgaggcccgt ttcggtccgc ccgggtccgg caagtcgagc
     1381 gacggtccgg atgggtggcc ctattacaac ctgcagttca ctggcggagg caccatcatc
     1441 gcggttggct ggcccggcca gtgggccagc tcgttcgtcc gggacgaggc gacgggactc
     1501 ggggtgcgtg ccgggcagga gttcacgcgg ctgtacctta gaccgggtga ggaggtccgt
     1561 acgccgctca ttgcgctgtt gttctggtcg ggcgacgatg tagttcggtc gcagaaccta
     1621 tggcggcgtt ggtatgtcgc gcataacatg ccccgggtgg acgggcggac gcagcgtgcg
     1681 gtggcccaga tccaggtcgg cggctctgag gccgacatcg cctatgtcca gtcctttctg
     1741 gacgcgggga ttcgggtgga cctgtgctgg cgcgatgcgg gcggctcgcg tgactcggtc
     1801 tggttccccg tgggcgatgg accctttcac gagccggacc ccgcgtggct gaactcgggc
     1861 acttgggatg tggacctaac caagtatccc cggggcttcc ggccgttcac tgagtggatc
     1921 cacgcgcatg gcatggagtt cgtgctgtgg ttcgagccgg agagggttgg cgaccccgac
     1981 tcgtggctgg gccggaacca ccctgaatgg ctgctcccga acaactgggg cggggccata
     2041 ctcaacgagg gggatccggc agcacgccag tggctgatcg atcacatcgg tgacatcatc
     2101 gcccgcgagg gagtggactg gtaccgggag gatatgaacg gagggggacc tgcaccagcc
     2161 tggcgcgccc acgatgcgcc ggaccgacag ggcatcacgg agaatctgta cgtgcagggg
     2221 cacttggcct actgggacgc gcttcgccgc cgccatccgc ggttgagaat cgactcgtgc
     2281 gcgtcgggcg gacgtcgcaa tgatctcgag accatgcgcc gggcggtccc tctgctccga
     2341 agcgacttcc agtggccgga gatgggcgat gtggccgaag ggaatcaggg ccatacctac
     2401 ggcctctcgg cgtggctgcc gttccagggt acgggcgtgt accagtatga cccctactcg
     2461 taccgaagct tttaccttcc ctcgttcggc atgggggtgc tcacgcccga gaaccgtgag
     2521 gcccagagca gggcctacag cgagtgccgg caagtgggac cgcggatgct gggcgactac
     2581 taccctctga ctccctacag ccgggatctg gaccgctgga tggcctggca gtttgaccgc
     2641 ccgaacgagg gcgacggggt cgtacaggct ttccgtcgtc ggcagtgcgc cgaggatgtc
     2701 atcaacgtgc ggctgtccgg cctcgacccg tatgcgcgct atgggcttac cgatctcgac
     2761 cgggcaggca cggctgaggc gagcggccgc gagctgatga ctgagggcct gcgggtggag
     2821 gtcagcgaga agcccggggc ggccatcatt ctgtacagca aggtgcgata gaccggccgc
     2881 gcgcggctcc gcgttgcccg tgcacagaga aaggcgcgcc ccatggcgga acgttgggac
     2941 gcgcctttca cgttggtggg gttcggtcta gtgcttgccg ggcgccagtt tgactccccg
     3001 gcgctcgtag gtgatgtatg actcgagcgc aatcatcttc gggtcgtcga gtgccagtgc
     3061 ctgaccctca agggggtgct gcagacacca attgatcatg tcgcgcagcg ggaccactcg
     3121 gccgagctgc tgctggaact tggggtacgt ctccgggggt gggttcgcgg cgttgggatg
     3181 gcactgcgcg caggccacgc cgttggttcc gagctcgccg ctggtccaca gcgcgcgtcc
     3241 ctctttgacc acggacatga acccgccctg ccagcgctcc tggtcctccg tcgtgaactc
     3301 atcggcccgg gccatccatg ccatcagccc gcccgccacc aatacacccg ccatggccac
     3361 agtcaggata cgtctcatcg tcgctctccc tttctagaag tggtcttgcg gcgggatccg
     3421 cttgtccttg tccgggaagt cgcggtccac gggtcgcttc ttcgccggat cgaagccgat
     3481 ggtccggtcg gtgttgttga agagcctgta attgagcgtc acgcggccgg cctccacgtc
     3541 gatcccctgc cacccggtgg cgtcgcgctc gaagaacggg ttggcccggt tcatcgggac
     3601 cgtgatcggc gggaagtcgg gctccgcttg ggcataggtc tccgggtacg gccacggcca
     3661 tgcggtggcc atcaccgcct ggaaggcgat gttcccgatc tggttgtact ggatctggtg
     3721 gacgtgccca tagaccaccg tgggcttacg g
//
LOCUS       DAOSPL010000266         3729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_24757_length_3729_cov_3.302123, whole genome shotgun sequence.
ACCESSION   DAOSPL010000266 DAOSPL010000000
VERSION     DAOSPL010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24757_length_3729_cov_3.302123"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /locus_tag="PLQ54_06220"
     CDS             <1..1313
                     /locus_tag="PLQ54_06220"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOS92889.1"
                     /translation="ARRREAAASADAGPAPSRNQEAKKGTRMAEHAARGARASGVMIS
                     RGSVVGGFVFLLFFALACAMQVSDLGKRQVSDDHDHDHEAEQKPENDPKLQQYMKAKG
                     GEAPAPGPTESIRHKRLAAEADAAAEAYDEARVAALAQADARGLSSDDARLAQGYKYK
                     QAMMTGDIGARVDAVLHLREDAKWWHHLRTGLQSPRAEIRRACANMLAFAYWPATAPD
                     LERIAQSDPDPDVREAAVAALGTGPRVVSSLDVLESLLRDRDAHIVKAAGIALASIGL
                     PEAIPYLLRAARHPDADVRGQAIAGLYDALRYSMAGRSGYPQEVISDAVKQAFADALS
                     DESIGARRSAVQAITALKWLEQEDRLLAMIDYEKEHDWKVRHLASRALGQLFPQQADA
                     KWAIVRGLRRANIMDPDQSVRFAAMAALREMGYDPGKWKAHESS"
     gene            1300..1818
                     /locus_tag="PLQ54_06225"
     CDS             1300..1818
                     /locus_tag="PLQ54_06225"
                     /inference="COORDINATES: protein motif:HMM:NF025210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOS92890.1"
                     /translation="MSRARPADRSGSRVAFERDRGVTHLAARHSVSHVSVCLRDPAAE
                     MVGVFRRLAEAEVNVYFVKIHEDCICFAVDTPALDTALRVLAEGSLEARSERDCTIVS
                     TVARSMRDMSGIMSRIMGCLNEAAIEVREFADSYNSVSCLVSASDAGAAIAALSAEFG
                     VALQAPSSEDPW"
     gene            1938..2984
                     /locus_tag="PLQ54_06230"
     CDS             1938..2984
                     /locus_tag="PLQ54_06230"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS92891.1"
                     /translation="MADAARVLIVDDEDLVVEAIRAALRDEGYEFSRAADGLQALALV
                     AERVPDLIILDVLIPGITGYEVCSILKNDPIGRFIPIMLVTGLSSTDEMVHGFDRGAD
                     DFIGKPFNAHELRARVRSLLRFKNLTDRLEHVSAVIETLANAVEARDRYTVGHARRVA
                     TLSAELARACGASDTELDDIRSAGILHDVGKIGTPEAVLNKPGALTAAERRQIEAHPV
                     LGGRICRPLRTSANFRSIIRHHHERLDGSGYPDGLKGDEIHMAARTVALCDIFDALTT
                     DRPYRKGLPQEVGLQIIRDEVKRGYWDRELFEVFVDLVGDGWHYEGTEFGMETEPAMP
                     MQGAIVPPVPVAAP"
     gene            2981..3295
                     /locus_tag="PLQ54_06235"
     CDS             2981..3295
                     /locus_tag="PLQ54_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014011770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HOS92892.1"
                     /translation="MTIGEFQQAIRSIYYERDRARGVLAAYAWLNEEAGELARAIRSG
                     DAGALTTEMSDVLAWLTTLANLLDVDLEAAASRFAQGCPKCGNTPCSCPDGAQSTLAE
                     CP"
     gene            complement(3303..>3729)
                     /locus_tag="PLQ54_06240"
     CDS             complement(3303..>3729)
                     /locus_tag="PLQ54_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOS92893.1"
                     /translation="FRALDLDMIGVGPYIPHPDTPLGQQVACQSPVSWSVPPTEQMAY
                     KVVALARIVRPDANIPSTTALATINQVEGRELGLQRGANVVMPNLTPPEYRMRYEIYP
                     GKACVGETAEQCRTCLRGRIESIGRTIGLGQGGRAKMAV"
BASE COUNT          621 a         1227 c         1264 g          617 t
ORIGIN      
        1 tggcgcgccg gcgcgaggcc gcggcctcgg ccgatgccgg cccggcgccg agccggaatc
       61 aggaagccaa gaagggcacc cgcatggccg aacacgcggc gcggggcgcc cgggcgtcgg
      121 gcgtgatgat ctcgcgtggc agtgtggtcg gagggttcgt ctttcttctg ttctttgcac
      181 tcgcgtgcgc gatgcaggtc agcgacctcg gcaagcgcca ggtctcggac gaccacgacc
      241 acgaccacga agcagagcag aagcccgaga acgaccccaa gctccagcag tatatgaagg
      301 ccaaaggtgg cgaggcgccc gcgccgggac ccaccgagtc gatccgtcac aaacggctcg
      361 cggccgaggc cgacgccgcg gccgaggctt acgacgaggc ccgcgtagct gccctcgccc
      421 aggctgacgc tcgagggctg tcctccgacg acgcccggct ggcccagggc tacaagtaca
      481 agcaggccat gatgacgggc gacatcgggg ctcgagtgga cgcggttctg cacctacgcg
      541 aggatgccaa gtggtggcac catctgcgca cagggctcca gtctccccgg gccgagatcc
      601 gccgtgcctg cgcgaacatg ctcgctttcg cctactggcc ggcgacggct cccgacctgg
      661 agcgcatcgc ccagtccgac cccgatcccg atgtccgcga ggctgccgtg gcggcgctgg
      721 gtaccggtcc gcgcgtggtg tcgagcctgg atgtcctcga gagcctactc cgcgacagag
      781 acgcgcatat cgtcaaggcg gcgggaatcg cgctggcgag cattgggttg ccggaagcca
      841 taccctatct gttgagggcc gccaggcacc cggacgccga tgtgcgaggt caggcgatcg
      901 ccggcctcta cgacgccttg cggtactcga tggccgggcg gtcgggttac ccgcaagagg
      961 tgatcagtga cgcagtcaag caggcctttg cggacgccct gagcgatgag tccatcggcg
     1021 cgcgccgctc cgcggtccag gcgatcacgg cgctgaagtg gctggagcag gaagatcgcc
     1081 tgctggcgat gatcgattac gagaaggagc acgactggaa ggtacgccac ctggcttcac
     1141 gcgcgctggg ccagctcttc ccccaacagg cagacgccaa gtgggcgatc gttcgaggcc
     1201 tccgccgggc caacatcatg gatccggacc agtcggtccg cttcgcggcc atggccgcac
     1261 tccgggagat gggctatgac ccggggaagt ggaaggccca tgagtcgagc taggccggcc
     1321 gaccggtcgg gttcgcgggt ggccttcgag cgcgaccgcg gcgtgactca tctggcagca
     1381 cggcacagcg tgagccacgt gagcgtctgt ctccgcgatc cggcagccga aatggtgggg
     1441 gtgttccggc ggctcgcgga ggccgaggtc aatgtctact tcgtgaagat acacgaggac
     1501 tgcatctgct tcgcggtcga caccccggcc ctggacaccg cgctacgcgt cctcgccgag
     1561 ggcagcctgg aggcgcggtc cgagcgcgac tgcaccattg tgtccacagt ggcccggtcg
     1621 atgcgtgaca tgtcgggcat catgtcacgg atcatgggat gcctgaatga ggcggcgatc
     1681 gaggtgcgtg agttcgccga ctcgtacaac tccgtctcct gcctggtctc cgccagcgac
     1741 gcgggcgcgg ccattgcggc gctgtctgcc gagtttggcg tagcgctgca ggcccccagc
     1801 tcggaggacc cctggtaagc cagccgcgtg tcggccacaa ctgcgatggc ggatctcacg
     1861 aactcagttt gtacatggcc cctgccatgg aactcttcta gcgatggaga gagccgtgtg
     1921 gttctgggga ggcggccatg gcggacgcag ccagagtcct cattgtcgac gatgaggact
     1981 tggtggtaga ggccattcga gcggcgttga gagacgaggg ctacgagttt tcgcgcgcgg
     2041 ccgacggcct ccaggcactg gccctcgtcg cggaacgggt gcctgatctc atcatcctcg
     2101 atgttctgat ccccgggatc acggggtatg aggtctgctc gatcctgaag aacgatccca
     2161 ttggtcggtt cataccgatc atgttggtga ctggattgtc atcaactgac gagatggttc
     2221 acggtttcga tcgcggcgcc gacgacttca tcggtaaacc cttcaacgcc cacgagctgc
     2281 gtgccagagt tcgctcgttg cttcgcttca agaatcttac cgatcgactc gagcacgtgt
     2341 cggcggtcat cgagacactg gcgaatgccg tcgaagcccg ggaccggtac accgtgggcc
     2401 acgcacgacg cgtggcaacc ctgagcgccg agctcgcccg agcgtgcggc gcatcggaca
     2461 ccgagctcga cgatatccgc tcggccggga tcctccacga cgtcgggaag attgggaccc
     2521 ctgaggccgt gctcaacaag cccggcgcac tgaccgccgc tgagcggcgt cagatcgagg
     2581 cccatcccgt gctgggtggc cggatctgcc gcccgctgcg gaccagcgcc aacttccggt
     2641 cgatcatccg gcaccaccac gagcgcctcg atggctcggg ctatcccgat ggcctcaagg
     2701 gcgacgagat ccacatggcc gcccgcacgg tggccctgtg cgacatcttc gatgcgctga
     2761 cgacggaccg gccataccgc aagggcctcc cgcaggaagt cggcctgcag atcatccgcg
     2821 acgaggttaa gcgcggctat tgggaccgag agctgttcga ggttttcgtc gacttggtcg
     2881 gcgatgggtg gcactatgag ggtaccgagt tcgggatgga gacggagccg gcgatgccca
     2941 tgcagggcgc cattgtcccc cccgtgcccg ttgcagcccc atgaccatcg gcgaattcca
     3001 gcaggcgatc agatccatct attacgagcg cgaccgcgcc cggggcgttc tggccgccta
     3061 tgcttggctg aacgaggaag ccggggaact ggcgcgggcc atccgttcgg gcgacgcagg
     3121 ggcgctcacc acagagatgt ccgatgtgct ggcttggctg acgaccctgg ccaatctact
     3181 cgacgtggac ctcgaggccg ccgccagccg ctttgctcag gggtgtccca agtgcgggaa
     3241 cactccctgc tcctgtcccg acggcgcgca gtcgacgctg gccgagtgcc cgtaggtggg
     3301 ccctataccg ccatcttcgc cctaccgccc tggcccaacc cgatggtccg gccgatgctc
     3361 tcgatccggc cccgcaggca cgtccggcac tgctcggccg tctctccgac acacgctttc
     3421 cccgggtaga tctcgtaccg catgcggtac tccggcggcg tcagattcgg catcaccaca
     3481 ttggcgccgc gctggaggcc cagctcacgg ccctcgacct ggttgatggt ggccagcgcg
     3541 gtggtgctgg ggatgttggc atcgggccgg acgatccgtg ccagggcaac caccttgtag
     3601 gccatctgct cggtgggagg cacactccaa ctgaccggcg actggcacgc gacttgctgg
     3661 ccgagcggcg tgtccgggtg cgggatgtag ggccccacgc cgatcatgtc gagatccaag
     3721 gcgcggaac
//
LOCUS       DAOSPL010000267         3727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_24771_length_3727_cov_3.176743, whole genome shotgun sequence.
ACCESSION   DAOSPL010000267 DAOSPL010000000
VERSION     DAOSPL010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24771_length_3727_cov_3.176743"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLQ54_06245"
     CDS             complement(<1..233)
                     /locus_tag="PLQ54_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOS92894.1"
                     /translation="MSPIVFDLNEDQQAILDAVREFCDDELVPKAEATDAAGVWPAEN
                     VAKLGELDLMGITVPEEYGGLGMDHITWTLVAE"
     gene            complement(558..1385)
                     /locus_tag="PLQ54_06250"
     CDS             complement(558..1385)
                     /locus_tag="PLQ54_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HOS92895.1"
                     /translation="MGVANEHSIAWGIAEAFRREGARLAFSYAVDSMERRVRPLGESI
                     GAAYIGPCDVREDEQLDAFFEEASAALGRIDTVVHAIAFSNKDDLRRPVHEVSRAGYL
                     EAIDISAYSLIGVAQAALPYMGTGSNLLTITYMGAEKVVPGYGLMGPVKADLEAIARY
                     LAASLGAYGIRVNAISAGPVRTLAARGIPRFGGLLDVAATASPLGCNVSTQCIGDVAA
                     CLCSPMFCSVTGEVIHVDCGFHIMGVPAQLEAWKEAGRAEALKEQEERASGVQSEAE"
     gene            complement(1856..2680)
                     /locus_tag="PLQ54_06255"
     CDS             complement(1856..2680)
                     /locus_tag="PLQ54_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS92896.1"
                     /translation="MKPWIMFTKHLEGWDLARIIDGLKRAGVQGADLCVRPGYPVNPE
                     NAATELPAAARRFADEGLCIPLVTTPGDFGDAGITYAEPLFAACAEAGVGLVKLGYWY
                     VEEGGYWETVDRCRTRLEGFAKLAERTGVKALVHNHSGATMGLNSSSVMNLVDGFDPD
                     HIGVFADVGHLSIVGEPYPMAFDIVGDYMAAVAFKDLVRTRIPQADGTAPWGIDVWPL
                     GMGYGDFPLVMRLLKERGFAGPISFHCEYSRMPAESIIHQCAIDTRYIQQILDAQA"
     gene            complement(2808..>3727)
                     /locus_tag="PLQ54_06260"
     CDS             complement(2808..>3727)
                     /locus_tag="PLQ54_06260"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOS92897.1"
                     /translation="TPPLGPCVTVRERDGDAEVCVEGEFFTRYVTHGGPKPYLYPIIG
                     PTGKPVTRDYPMKEDTPGEDHDHVHHRSLWFTHDQVNGVNFWAEGEQAGRTVHRAFES
                     LESGPVMGRVVATTDWIGPDGRKICEDRRDMRIYATAGARVLDFDITWRASEGPLHLG
                     DTKEGVFGVRVAETMKVDRGQGHIVMSTGYRDGEAWGKAAEWCDYSGPVDGQTVGIAI
                     LDHPGNPGHPTHWHVRTYGLFCANIFGLSYFEPGSGKDGSLDLKPGEALRFRYRVLIH
                     PGGAEEAGLSDAYAVYATPPEVTVRPMER"
BASE COUNT          641 a         1343 c         1142 g          601 t
ORIGIN      
        1 tcctcggcca ccaacgtcca ggtgatatgg tccatgccca agccgccgta ctcctcgggc
       61 acggtgatgc ccatcaggtc gagctctccg agcttggcga cgttctccgc gggccagacg
      121 cccgccgcat ccgtcgcctc ggccttgggc acgagttcat catcacaaaa ctcccgcacg
      181 gcatccagga tggcctgctg gtcctcgttg aggtcgaata cgatcggact caaccccctt
      241 ctgtctgccc agcaaccgcc actcaggcag acctcttccc ccggcggagc gtctgggggc
      301 cgggcgtcat gattgtccag gttcaggatg cgtgtcaagg cagaccacgc tcccccgggc
      361 gcgcacgcac cacgcgctct gactcggcaa tggactgcca cgccccggcg cgagttggcc
      421 gtacattgac ccggtaccta cccgaagaca ggaacccgga cgctggcgag gttggcctcc
      481 acgatcatct gctcggcggc tcgtgtgggg tcgtgctggc ctcacacacg gagcagccgg
      541 tggctggcaa ggccccctca ctccgcctca gactggacgc ccgacgcgcg ctcctcctgc
      601 tctttgaggg cctcggcgcg accggcttcc ttccacgcct cgagctgcgc gggaacaccc
      661 atgatgtgaa atccgcagtc cacgtggatc acctcgcccg tcaccgaaca gaacatgggc
      721 gagcacaggc atgcggccac atcgccgatg cactgagtcg acacgttgca cccgagaggc
      781 gaagccgtcg ccgccacatc cagcagtccg ccgaagcgcg ggatgccccg ggcggccaac
      841 gtgcgcaccg gccccgccga gatggcgttc acgcgaatgc cgtacgcgcc caggctggcg
      901 gccagatagc gggcgatggc ctccagatcc gccttcacgg gccccatgag gccataaccc
      961 ggcaccacct tctcggcacc catgtaggtg atggtgagaa ggtttgaccc tgttcccata
     1021 tagggcagag ccgcctgggc cacgccgatg agagagtagg cggagatatc gatggcctcc
     1081 aggtagcccg cgcggctcac ctcgtggacc ggtcgccgca ggtcatcctt gttcgagaag
     1141 gcgatggcat gaacgaccgt gtcgatccgg cccagcgcgg cagaggcctc ctcgaagaag
     1201 gcgtccagct gctcatcctc ccgcacatca catggcccga tgtacgcggc cccgatggac
     1261 tcgccgagag gccgaacgcg ccgctccatg ctatccacgg catagctgaa cgccagccgc
     1321 gcaccctcgc gccggaacgc ttcagcgatg ccccaggcga tggaatgctc attggccacg
     1381 cccatgatca cggcattcct gccggataga agacccattg gtacggcacc tttcccatct
     1441 ctggcccgcg aacgaatgag cagtacgcgc acagccagtg gcatacgacg cggccctcgg
     1501 ccgacaccca atgggcgcgc gatccatgag gagtacccgc acgcagtccc cgaaggcggc
     1561 cgcgccggcg aaggggagtg actccggtgg aacccgcaca gaacccgtcg ttcgcgctgc
     1621 gcctcaacta ccgaaagcag tatatcctac tacagtctgt gctgacgaga agccgccccg
     1681 gggcggccgt ccgcccgccg ccagactcgg caacgatcgg atacacgcac tccgagttgc
     1741 gcttcagccc ggcccctgct acggcgacga tgctgacgtg ttcgacgccg cgccatgcag
     1801 accaagagcg atggccatgg tcagggccgg atcagcagca tggctctcgc tcctgctacg
     1861 cttgcgcgtc caggatctgc tggatgtacc gggtgtcgat cgcgcactgg tggatgatcg
     1921 actcggccgg catgcggctg tactcgcagt ggaagctgat cggccccgcg aagccgcgtt
     1981 ccttcaggag gcgcatcacc agaggaaagt cgccgtagcc catgcccagc ggccacacat
     2041 cgatccccca cggggctgtg ccgtcggctt gggggatccg ggtgcgaacc aggtccttga
     2101 acgccacggc cgccatgtag tcgcccacga tgtcgaaggc catcgggtag ggttcgccca
     2161 caatcgatag atgcccgaca tcggcgaaca cgccgatgtg gtccgggtcg aagccatcca
     2221 ccaggttcat caccgacgac gagttcaacc ccatggtcgc gcccgaatgg ttgtgaacca
     2281 gggccttgac gcccgttctc tcagccagct tggcgaaacc ctccaggcgc gtccggcacc
     2341 ggtctacggt ctcccagtag cccccctctt ccacatacca atagcccagc ttcaccaggc
     2401 ccacccccgc ctccgcacac gcggcgaaca gcggctcggc gtaggtgata cccgcatcgc
     2461 caaagtcacc gggtgtggtg acgagcggga tgcagagccc ctcatcggcg aagcggcgtg
     2521 cggccgcggg cagttccgtg gccgcattct ccgggttcac cggatagccg ggccggacac
     2581 acagatccgc gccctgcacg ccggcgcgct tcagaccgtc gatgatccgc gccagatccc
     2641 agccctcgag gtgcttcgtg aacatgatcc acggcttcaa ggcacgtcct cttctctacc
     2701 cctgcgtggc ctccggccgc catacagcgc ccgggtagcg ttcggccgtc gtgcagggcc
     2761 atccccgccg tctcattggc cgtgcggcat cgtccggact ccggccccta gcgctccatc
     2821 ggccgcaccg tcacttcggg cggcgtcgcg tagaccgcgt aggcatcaga gaggccggcc
     2881 tcctccgcgc cacccgggtg gatcaggacc cgatagcgga agcgcagggc ctcgcccggc
     2941 ttcaggtcga ggctgccatc ctttcccgac cccggctcga agtacgacag cccgaagatg
     3001 ttggcgcaga acaggccgta ggtccgcaca tgccagtggg ttggatggcc gggattgccc
     3061 gggtggtcca gaatggcaat gcccaccgtc tggccgtcga ctgggcccga atagtcgcac
     3121 cactcggccg ccttgcccca ggcctcgccg tctcggtaac cggtggacat gacgatgtgg
     3181 ccctgccccc ggtccacctt catggtctca gccacgcgga cgccgaacac gccctccttg
     3241 gtgtcgccca ggtgcagcgg gccctcgctc gcgcgccagg tgatatcgaa gtcgagcacc
     3301 cgtgccccgg ccgtcgcgta gatgcgcata tcgcgccggt cctcgcagat cttcctgcca
     3361 tcggggccga tccagtccgt cgtggcgacc acacgcccca tcacgggccc actctcgagc
     3421 gactcgaacg cgcggtgcac cgtgcggccc gcctgctccc cttcggccca gaagttgacg
     3481 ccgttgacct ggtcgtgagt gaaccacagc gatcggtggt gcacgtggtc atggtcctca
     3541 ccgggcgtgt cctctttcat cggatagtcc cgagtcacgg gcttcccggt gggcccgatg
     3601 atgggataga ggtacggctt gggcccgcca tgggtgacgt aacgtgtgaa gaactcgcct
     3661 tcgacacaga cctccgcatc accgtcacgc tccctgacgg ttacgcaggg gcccagtgga
     3721 ggagtgg
//
LOCUS       DAOSPL010000268         3722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_24808_length_3722_cov_4.638942, whole genome shotgun sequence.
ACCESSION   DAOSPL010000268 DAOSPL010000000
VERSION     DAOSPL010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24808_length_3722_cov_4.638942"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLQ54_06265"
     CDS             <1..215
                     /locus_tag="PLQ54_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92898.1"
                     /translation="PAADVLADRHLEIITQLQRGLATQAWAAPRLASINLALDLMRHP
                     SQRALAWSRTDTWPEVRNVREQRLTA"
     gene            301..591
                     /locus_tag="PLQ54_06270"
     CDS             301..591
                     /locus_tag="PLQ54_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92899.1"
                     /translation="MTDREKRQILKLTARWRRIAKRTADALERMKWGTTSLVRTLEPL
                     KHMPVLDGLYLTALRWDCRVGDDLRDLRDEIAAATQQTRKYNPPGQLTLLPV"
     gene            764..1255
                     /locus_tag="PLQ54_06275"
     CDS             764..1255
                     /locus_tag="PLQ54_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92900.1"
                     /translation="MRKPTQFETLRCAASHTWQAPLRDAVLGDCTRYGVKLVCEGHET
                     CPDCGLLPGTQEYCWVCDEPLSQERIGEIAAAADHPEALGPRDFLCAAHAEAVAGDDC
                     NARRVSAMRLGALAAFVELRLDEAELAGDAEAMCEECPAQHPDCALGCATRLLAWLRE
                     GER"
     gene            1258..2070
                     /locus_tag="PLQ54_06280"
     CDS             1258..2070
                     /locus_tag="PLQ54_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOS92901.1"
                     /translation="MVAAVAEPTYMPDSYHVGSPCLGASKIADYLKCPLSYRLKYVDK
                     IPAPKSPHAFAGTVIHQVAHHINMCDWTADHAQEAADMLWGLWEQGRLSTSDPDNPEI
                     VRSIGEAANVWLPWYLHWREGQRQLVSEHHWLLQIEPNELGCDHDRIVLEGTIDRAYR
                     EGGLVIVSDMKSGARKPSQADLDRDLQLSVYSFAARRQGLREDAMELVWLRTQETLRT
                     TRTDEYLLAVMRQVVLPVARAIAGGVFPANPASKYGCGYCDHQEHCEVGRAA"
     gene            2105..3235
                     /locus_tag="PLQ54_06285"
     CDS             2105..3235
                     /locus_tag="PLQ54_06285"
                     /inference="COORDINATES: protein motif:HMM:NF039495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92902.1"
                     /translation="MLKGFYRSDQLLRLGKIRLGEKTISAGSGREHPKDLQHFLVPPE
                     VEAVTGPNPTTLKVMLPSADWEQVCPTALEKWVKWGEDRRLMCKGDGQLVIKRRDENM
                     NWLDGDDDLPCAYKACPEYLAGNCTEVGRLNVILPDVNLLGVYTIDTGSFYGLNQVYA
                     AFASAVMLLENLIGNPELITRAECVLSREMTSLEYQDPKSGQRKQVSKAILKLTIPPI
                     SMPAVRSLQAASPLQQLPAGELPDLPPAPGYTPGPVAAPEMPPPDESCPEDIAPGMAP
                     VGPDLGQKAAWDVLVEQVQALGKNAVVFERSVCEAIRPDAARFEDLAGETEAAQALTQ
                     GAQMIEGWQQASQPTQAAEARKQAARKAAAPKPRSGGEGLQF"
     gene            3279..>3722
                     /locus_tag="PLQ54_06290"
     CDS             3279..>3722
                     /locus_tag="PLQ54_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS92903.1"
                     /translation="MIDQVLARNFRGLSAADIHLDPCLTLVLGPNGSGKSSLCGAIEY
                     ALTGACEWTDGAGRGASVLIAHGSAGASVSVGGAQLGTIERQITAKGSSVAFDSRTGK
                     EAEAAIGAALPGSALLPAMLRSDGFLRLSGKEQQDLLFGLSGGETD"
BASE COUNT          643 a         1283 c         1302 g          494 t
ORIGIN      
        1 gaccggccgc cgacgtgctg gccgaccggc acctggagat catcacgcaa ctgcagcgcg
       61 gcctggcgac gcaggcctgg gcggcgccga gactggcaag catcaacctg gcgctcgacc
      121 tgatgcggca tccgtcgcag cgggcattgg cgtggagcag gaccgacacg tggccggaag
      181 tgcggaacgt gcgggagcag agattgacgg cgtagtaccc gcgccgaggg ggcgcgttca
      241 catagccgcc tgggttcccg cttgtgcggg agcattcgac aggggaagga gggccacgtc
      301 atgaccgacc gcgagaaacg ccagatcctc aagctgaccg cgcgctggcg ccgcattgcc
      361 aagcgcaccg ccgatgctct ggagcggatg aagtggggca ccacctcgct cgtgcgcacg
      421 ctggagccgc tgaagcacat gccggtgctg gacgggctgt acctgacggc cctccggtgg
      481 gactgtcgtg ttggcgacga cttgcgcgac ctgcgtgacg agatcgccgc cgcgacgcag
      541 caaacgcgga agtacaaccc accggggcag ttgacgctgc tgccggtctg acacaccgcc
      601 gttcttacgc tcgtcacgct cttcccgaga ggggtgacac gagtatgggg cgaccgtaag
      661 acggccctga gccacacgcc ctaacgagat gcgagtttct ggagtggggc gggggatggt
      721 ggggcgatca ccttcccccg ccctgagcaa agagagagac accatgcgca aaccaacaca
      781 gttcgagacc ctccgctgcg ccgccagcca cacctggcag gccccgctgc gtgacgcggt
      841 cctgggcgac tgcaccaggt acggcgtcaa gctcgtgtgc gaggggcacg agacctgccc
      901 cgactgcggg ctgctgcccg gaacgcagga gtactgctgg gtctgtgacg agccgctgag
      961 ccaggagcgc atcggcgaga ttgccgccgc cgcggaccat cccgaggccc tggggccgcg
     1021 cgacttcctc tgcgccgccc atgccgaggc ggttgccggc gacgactgca acgcgcggcg
     1081 tgtcagcgcc atgcgcctgg gcgcgctggc cgcgttcgtc gagctgcggc tggacgaagc
     1141 ggagctggcc ggcgacgccg aggccatgtg cgaggaatgc ccggcgcagc accccgactg
     1201 cgccctcggc tgcgcgacgc gcctgctggc gtggctgcga gagggggagc ggtagagatg
     1261 gtcgctgccg tcgccgagcc cacctacatg cccgacagct accacgtggg cagcccgtgc
     1321 ctgggggcga gcaagatcgc tgactacctc aagtgcccgc tgtcctaccg gctcaagtac
     1381 gtggacaaga ttcccgcgcc caagtcgccc cacgctttcg ccggcacggt catccaccag
     1441 gtcgcccacc acatcaacat gtgcgactgg acggcggacc atgcgcagga agccgcagac
     1501 atgctgtggg gcctgtggga gcaggggcgc ctgtctacca gcgaccccga caaccccgag
     1561 atcgtgcgca gcatcggcga ggccgcgaac gtctggctgc cctggtacct gcactggcgg
     1621 gaggggcaga ggcagctcgt cagcgagcac cactggctgc tgcagatcga gccgaacgag
     1681 ctgggctgcg accatgaccg catcgtgctc gagggcacga ttgaccgggc ctaccgcgag
     1741 ggcggactgg tgatcgtctc cgacatgaag agcggcgccc gcaagcccag tcaggcagac
     1801 ctcgaccgcg acctgcagct ctcggtctac tcgttcgcgg cccgccgcca ggggctgcgc
     1861 gaggacgcca tggagctggt ctggctgcgc acccaggaga cgctgcgcac gacccgcacc
     1921 gacgagtacc tgctggcggt catgcgacag gtggtgctgc cggtggcgcg ggccatcgcg
     1981 ggcggcgtct tcccggccaa cccggccagc aagtacggct gtgggtactg tgaccaccag
     2041 gaacactgcg aggtaggccg cgccgcgtag cgcgccaccc aacacgacag ggagagtgaa
     2101 gacgatgctc aaaggcttct accgtagcga ccaactgctg cgcctgggga agatcaggct
     2161 gggggagaag accatctcgg ccggcagcgg gcgcgaacat cccaaggacc tgcagcactt
     2221 cctggtcccg cccgaggtcg aggccgtcac cggccccaac ccgacgacgc tgaaggtcat
     2281 gctgccgagc gcggattggg aacaagtgtg cccgacggcc cttgagaagt gggtcaagtg
     2341 gggcgaggac cgccggctca tgtgcaaggg cgacgggcaa ctcgtcatca agcgccgcga
     2401 cgagaacatg aactggctcg acggcgacga cgatctgccc tgcgcctaca aggcctgccc
     2461 tgagtacctg gccggcaact gcacggaggt cgggcgcctg aacgtcatcc tgccggatgt
     2521 gaacctgctg ggcgtctaca ccattgacac cggcagcttc tacgggctga accaggtgta
     2581 cgcggccttc gcctccgccg tgatgctgct ggagaacctc atcggcaacc ccgaactcat
     2641 cacccgcgcc gagtgcgtcc tgtcccgcga gatgacatca ctggagtacc aggaccccaa
     2701 gagcggccag cgcaagcagg tcagcaaggc tatcctcaag ctgaccattc cgccgatctc
     2761 catgcccgcc gtgcgctcac tgcaggcggc cagcccgctg cagcagttgc cggccggcga
     2821 gctgccggac ctgccgccgg ctccgggcta cacgcccggc cccgtggccg ctcccgagat
     2881 gccgccgccc gacgagtcct gccccgagga catcgccccg ggcatggcgc ccgtcggccc
     2941 cgacctgggc cagaaggccg cctgggacgt cctggtcgag caggtgcagg ccctggggaa
     3001 gaatgccgtg gtcttcgaga ggtcggtctg cgaggccatc cggcccgacg cggcgcgctt
     3061 cgaggacctc gccggcgaga cggaggccgc gcaggccctc acgcagggcg cgcagatgat
     3121 cgagggctgg cagcaggcca gccagccgac tcaggccgcc gaggcccgca agcaggcggc
     3181 gcgcaaagcc gccgcaccca agcccaggtc cggcggcgag gggctgcagt tctgaggacc
     3241 gcagggcaag ctgagcacat cacagggaga gacctgacat gatagatcaa gtgctggctc
     3301 gcaacttccg cggcctgagc gccgccgaca tacacctcga cccgtgcctg acgctggtgc
     3361 tcgggccgaa cggatcgggc aagtccagcc tctgcggggc catcgagtac gccctgacgg
     3421 gggcctgcga gtggaccgac ggagcggggc gtggcgccag cgtcctgatt gcccacggct
     3481 ccgccggcgc gtccgtcagt gtgggcggcg cgcagctcgg caccatcgag cggcagatca
     3541 ccgccaaggg cagcagcgtc gccttcgact cccgcaccgg caaggaggcc gaggctgcca
     3601 tcggggcggc gctgcccggc tccgccctgc tgcccgcgat gctgcgctcc gacggcttcc
     3661 tgaggctgtc ggggaaggag cagcaggacc tgctcttcgg cctcagcggc ggggagacgg
     3721 ac
//
LOCUS       DAOSPL010000269         3721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_24820_length_3721_cov_3.125477, whole genome shotgun sequence.
ACCESSION   DAOSPL010000269 DAOSPL010000000
VERSION     DAOSPL010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24820_length_3721_cov_3.125477"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PLQ54_06295"
     CDS             <1..389
                     /locus_tag="PLQ54_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92904.1"
                     /translation="WRRWSEWGAYPADSISRVEGTASAHREPGGGPDIEGTPPRWPWA
                     LDETELGTTDFRSVKLNVYRASLKAPNGQGVALDADADAHVRACLAPGGVWMHLLKTC
                     RLGPVTVHDGDRLQGEFALSLLAGRS"
     gene            complement(610..3492)
                     /locus_tag="PLQ54_06300"
     CDS             complement(610..3492)
                     /locus_tag="PLQ54_06300"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOS92905.1"
                     /translation="MAQDIAVPSGAASAKAASDRTLAWAFAGCFFFSGCTGLVYQNVW
                     VRILGLHFGNTAVAISAVLTAFMAGLALGSYIIGERADRLRHPLRAYAIIEILIGLYC
                     VISPVLLTAARHAYFGFIRGGDSGVVARTLIQFTLCFVVLVIPTALMGATLPVLSAGL
                     QRVMGTVAKTVGNLYAVNTFGAVAGTLLTAFFLLPTIGVQFTIYAGAIVNVSIGILLL
                     IVGRKAPAPEPVADVTRVSIEEAEGDPGSSPDGAPPVAVAVRPSVIHRFMLWGFFVAG
                     FCSLCSEVVWTRVLSMTIGSSVYAFAVILTAFLLGIALGSYLFALIWGKRPVGIHTFG
                     LTEVLIGILCILLTQAFAWMPEVALSVLRGLKDSAPGALLAVQLIVAFLLVAPSTILM
                     GIAFPLVTKILTDAGGPLGRSVGRAYAANTAGCILGSALSSLLLVPLLGMRVTILGAA
                     VLYVLVGCGAFLIATERRALWRTWAGVSVAAAALLVAVLPSWSHSAFAFGVFQSNVVM
                     GDDPAGGLAYARSNPERVKFYREGPSAVVSVVSWGDGKYALGVNGKTDASTSKGDMVT
                     QVMTGHVPCLLAKELKRVVLVGCGSGISAACIARHPSVQSVECVEIEPNVGEAARQWF
                     GDLNDHIFDREPRFKFHVDDGRSFIERVTEPYDVIISEPSNPWIAGVGNLYTVENYEY
                     CRRALAEGGIMVQWTQIYSMGNDSLRMILNTFSSVFPHVAVFHTTSSDLLLVGSNEPL
                     VLDRNKLQERLNALKDRADLAEFGCDTPEGFSSSYLLAGKDVQKLITKARKNTDDLPL
                     LEFWAPRSLYSWTARENFQCLLAEQSSDLPPMQGFATRGPRGAHVRLLRGRSFRLRLE
                     NESGLARREFEKALELDPTSVPVLLELAQCAQDESRSVAALDYLDRALAIDPGEAEVH
                     YRRALILADQGLTGNAIEALRLATALAPTNRDYQDKLDELREQSRR"
BASE COUNT          684 a         1177 c         1325 g          535 t
ORIGIN      
        1 gctggcgccg ctggtcggag tggggggcct acccggccga tagcatcagc cgcgtcgagg
       61 gcacggccag cgcgcaccgt gaacccggtg gcgggccgga catcgagggc acaccgccgc
      121 gctggccgtg ggcactggac gagaccgagt tgggcacgac cgacttccgc tccgtgaagc
      181 tcaacgtgta ccgtgcgtcg ctgaaggcgc cgaacgggca gggcgtggca ctcgatgccg
      241 acgcggacgc ccacgtgcgg gcatgcctgg ccccgggcgg cgtctggatg cacctgctga
      301 agacgtgccg cctcggcccg gtgactgtgc acgatggcga ccggctgcag ggcgagttcg
      361 cgctaagcct gctggcgggg cggagctgac ccgcggggcg ctgccccgca ccgcgccgga
      421 cgacgcagaa ccgcgggccg ctgccccgca ccccgccagg ggacccgtcc cctggacccc
      481 agccgactgc cgcgcggttc cccgagggct ccggccgccc gatcgccgag caggggcgac
      541 cggcaggtcg cccagggcgg gcgttgtggc cgacacagca gggcgcagca ccacgcgagt
      601 ccgcctctgc taccgtcgtg actgctccct cagctcgtcg agcttgtcct ggtagtcccg
      661 gttggtcggg gcgagggcag tcgccaagcg gagggcctca atggcgttgc cagtgaggcc
      721 ctgatcggcc aggatcagcg cgcgacggta gtggacctcg gcctcgcccg ggtcgatggc
      781 caaagcgcgg tcgaggtagt cgagcgccgc caccgagcgg ctttcatcct gcgcgcactg
      841 ggccagttcg aggagcacgg gtacggacgt cggatcgagt tcgagggcct tctcgaactc
      901 acgacgggcc aggcccgact cgttctcgag gcgcagccgg aacgagcgcc cacgaaggag
      961 gcggacgtgg gcgccgcgag gaccacgggt cgcgaagccc tgcatgggcg gcagatcgct
     1021 gctctgctcg gccagcaggc actggaagtt ctcgcgagcg gtccacgagt acagtgaccg
     1081 aggtgcccag aactcgagca gcggcaggtc gtcggtgttc ttccgtgctt tggtaatgag
     1141 cttctgaaca tccttgccgg cgagcaggta ggaggacgag aagccctcgg gcgtatcgca
     1201 accgaactcg gccagatcgg ctcggtcctt gagtgcgttc aggcgctcct ggagcttgtt
     1261 gcggtcgagc accaggggtt cgttcgagcc cacgagcagc aggtcggacg atgtggtgtg
     1321 gaacacggcc acgtgtggga agacgctgct gaaggtgttc aggatcatcc gcaggctgtc
     1381 gttgcccatg gagtagatct gagtccactg gaccatgatg ccgccctcgg caagggcgcg
     1441 ccggcagtac tcgtagttct cgacggtgta gaggttgccg acgccggcga tccacgggtt
     1501 cgagggctcg gagatgatca catcgtaggg ttcggtgacg cgctcgatga agctgcggcc
     1561 gtcgtcgacg tggaacttga agcgaggttc ccggtcgaag atgtgatcgt tgaggtcgcc
     1621 gaaccactgc cgggccgcct cacccacgtt gggttcgatc tcgacgcact cgacggactg
     1681 gacgctgggg tgccgggcga tgcatgcggc actgatgcca ctcccgcagc cgacgagcac
     1741 cacccgtttg agctccttgg ccaggaggca gggcacatgg cccgtcatga cctgggtgac
     1801 catgtcgccc ttggaggtcg aggcatccgt cttgccgttc acacccaagg catacttgcc
     1861 atcgccccag ctcacgacgc tcaccacggc actggggccc tcgcggtaga acttcacgcg
     1921 ctcagggttc gaccgcgcgt aggcgagccc gccagccggg tcgtccccca tgacgacatt
     1981 ggactggaag acaccgaatg cgaatgccga gtggctccag gagggcagca cggcgaccaa
     2041 cagggccgcc gcggcgacgc tgacgcccgc ccaggtccgc cacagggccc ggcgctcggt
     2101 ggcgatcaga aacgctccgc acccgacgag gacgtagagc accgcagcac cgaggatcgt
     2161 gaccctcatg cccaggagtg gcaccaggag cagtgaggag agggccgagc ccaagatgca
     2221 gcccgcagtg ttggcggcat aggctcgacc cacactgcgc cccaacgggc cgccggcgtc
     2281 ggtgaggatc ttggtgacca gcgggaacgc aatgcccatc aggatggtcg acggtgccac
     2341 caggaggaaa gcgacgatga gctggacggc aaggagcgca ccgggcgccg agtccttcag
     2401 tccgcgcagg accgagagcg cgacctcggg catccacgcg aatgcctggg tcaacaggat
     2461 gcacagaatg ccgatgagca cctcggtcag tccaaaggtg tgaatgccca cgggccgctt
     2521 gccccagatg agcgcaaaga ggtagctgcc gagggcgatg cccaagagga atgcggtgag
     2581 gatcacggcg aaggcgtaga ccgaactacc aatggtcatg ctgagtacgc gagtccagac
     2641 gacctcggag cacagcgaac agaagcccgc gacgaagaag ccccacagca tgaaccggtg
     2701 gataacgctg gggcgaaccg ccacggccac aggcggagct ccatcggggg aagaccccgg
     2761 gtcgccttcg gcctcctcga tcgacacgcg ggtgacatcg gccacgggct cgggcgccgg
     2821 ggctttgcgg ccgacgatca gcagcagaat gccgatggag acattgacga tggctccggc
     2881 gtagatggtg aactgcacac cgatggtggg cagcaggaag aaggcggtca acaacgttcc
     2941 ggctaccgcg ccgaatgtgt tcacggcata gaggttgccc accgtcttgg ccaccgtgcc
     3001 catgacgcgc tgcaggccgg cgctgagaac gggcagagtg gctcccatga gtgccgtggg
     3061 gatgacgagc acgacgaagc agagcgtgaa ctggatgagc gtgcgggcca cgacacccga
     3121 gtcgccgccg cggatgaagc caaagtaggc gtgccgtgcc gcggtgagga gcaccggcga
     3181 tatgacgcag tagaggccga tgaggatctc gatgatcgcg taggcgcgta gcggatggcg
     3241 cagccggtcg gcacgctcgc caatgatata gctgcccagg gcgagcccgg ccatgaatgc
     3301 ggtgagaacg gcactgatgg ccaccgcggt gttgccgaaa tggagaccca gaatcctgac
     3361 ccagacgttc tggtagacca gaccggtgca gcccgagaag aagaagcagc cggcgaacgc
     3421 ccacgcgagg gtgcggtcgc ttgccgcctt ggccgatgcg gctcccgatg gaaccgcgat
     3481 gtcttgtgcc acgatgacgc ctcctggtcc gagagagacg gcggggggtg acgaagcacc
     3541 gcaccgccac gtgctggtct ggcaatgcct cttcgggtaa ccgggctcgt ccccttcaag
     3601 gcgttgccgg acagctggat agacattcag ccgctgatag cccgcgcacc tcccaccgca
     3661 cgcctacgtg agagctcaga gttggtgtgg gtgaagttgg tgtgggcgtg gggtgtcagg
     3721 c
//
LOCUS       DAOSPL010000270         3717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_24852_length_3717_cov_2.861278, whole genome shotgun sequence.
ACCESSION   DAOSPL010000270 DAOSPL010000000
VERSION     DAOSPL010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24852_length_3717_cov_2.861278"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PLQ54_06305"
     CDS             complement(<1..641)
                     /locus_tag="PLQ54_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOS92906.1"
                     /translation="MNVLVLLAVGVGALWLGSHFYGGYVSRRVGLSPDQPTPARQLND
                     GRDYVPTRTPVVFAHHFSAIAGAGPIVGPTMALIYGFAPSLLWVLLGGVLIGAVHDFT
                     ALFASLREGGRSMAQIARRTMGRSGFALFISFTTVMIVLVTAAFLNLTKTALTSVYPL
                     TALGLDPSQTLLRVEMKDGVMVGRIGGVASTSVLIITGLSPLMGYLLYRRRIAT"
     gene            complement(742..1743)
                     /locus_tag="PLQ54_06310"
     CDS             complement(742..1743)
                     /locus_tag="PLQ54_06310"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HOS92907.1"
                     /translation="MRRDRFDFLEIAEAPQAPVEEEILLPSINDLSSQSTAPTARHHA
                     SGGRLALLETIGEAGQEIGRFSSPSGLCIDGAENLYVADTMRHRIQKITPRDEAYGYG
                     GVGEGAGQFRMPSDVVVDPRLFIYVLEMGNHRITKLTQDGTYMCSIGGRGEGFGRFRS
                     PEAVCMDRFNNLLVADTGNHLIQVLDARGRFMEQYAFERAGGLRSPRGVWAGPDGEII
                     VADTGNGRLVHADGSGTVTGVFRGVEQCPLRAPVSACRDADHSIWVVDAEHHAILKFA
                     VDGALLARVGPMLGPNGNLCSPSGMVFAPNGDFYVADTGNHRLLRFGYRSARNRRTG"
     gene            complement(1754..3403)
                     /locus_tag="PLQ54_06315"
     CDS             complement(1754..3403)
                     /locus_tag="PLQ54_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS92908.1"
                     /translation="MPESQAREIEILVRARYPLVYVVSWEEARVEDVLAAVARARSKK
                     LFIWTVTDGLRPFNTPRGDEESRDPLVVLDRIVASKDQAVFVLKDFHPFLHDPQYATA
                     VIRKLRDATFALKMSYKTIIIVSPVLRLPPELEKAITVVDFGLPTFEDMGALLDRIIE
                     SVKGNKQINTDLSPEERDKLLKAALGLTLAEAENVFARSIVERKCLDVDVVLLEKEQI
                     IRKTEVLEYLRTETDVGSVGGLDLLKSWLGKRTRAFEESARQFGLPEPKGLLLLGVQG
                     CGKSLTAKSIAHLWRLPLLKMDVGRIFAGLVGASEENMRKAIRIAESVAPCVLWIDEI
                     EKGLSGVQSSSSSDAGTTARVFGTLLTWLQEKTAPVFVIATANDISQLPPELLRKGRF
                     DDIFFVDLPGITERKEIFRIHLARRGRDVERFDLERLAAVSAGFSGAEIEQAVIAGMY
                     DAFDKGAEVTTDDIVTAVQQTFPLCRTMAAEIDFLRRWAQNRARPSSSEPSEPLPDLG
                     PEDYGIMMQPGVAEAAAAAYRAFEAERRATAVGPPDVEDEE"
     gene            complement(3509..>3717)
                     /locus_tag="PLQ54_06320"
     CDS             complement(3509..>3717)
                     /locus_tag="PLQ54_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOS92909.1"
                     /translation="HISRLLVGPDHRRLLPVAALGGAVLLLIADLLSRTMPGFQEVPV
                     GIVTSVLGCPFFLLLLCQSRQTRF"
BASE COUNT          678 a         1274 c         1187 g          578 t
ORIGIN      
        1 gtggcgatcc ggcggcggta cagcaggtag cccatcagcg gcgagagtcc cgtgatgatc
       61 aacacggagg tcgacgcaac gcccccgatg cggccgacca tcacgccatc cttcatctcg
      121 acgcggagca gcgtctgcga cggatcgagc cccagggccg tgagcgggta gaccgaggtg
      181 agggccgtct tggtcaggtt gaggaaggct gccgtgacga ggacgatcat caccgtcgtg
      241 aagctgatga acaacgcgaa gccggatcga cccatggtgc gtcgggcgat ctgggccatg
      301 gaccgcccgc cctcgcgaag gctcgcaaac agcgccgtga agtcatgcac cgcaccgatc
      361 aagacgccgc cgaggaggac ccaaagcagc gacggggcga agccgtagat cagcgccatg
      421 gtgggtccca cgatgggccc cgcgccggca atcgcagaga agtggtgggc gaagaccacg
      481 ggagtgcgtg tcggtacgta gtcgcgtcca tcgttgagct gacgcgccgg agtcggctga
      541 tccggcgaca gccccacccg gcgcgacacg taccctccgt agaaatggct ccccagccac
      601 agcgcgccca cacccacagc cagcaacacc agtacgttca tgccccggcc tccctgcggc
      661 gcatgatacc agttccgcgg gacagccacc caggtggcgc gggctcggga gcgcgacgcg
      721 ggcggctccg cctctcggcc ctcaccccgt ccggcggttg cgggccgagc ggtagccgaa
      781 gcgcagcagg cggtggttgc ccgtatcggc aacgtagaag tcaccgttgg gcgcgaacac
      841 catccccgag ggcgagcaca ggtttccgtt cggccccagc atcggcccga ctcgagccaa
      901 cagagcgcca tcgaccgcaa acttgaggat ggcgtgatgc tccgcatcca ccacccagat
      961 gctgtggtcg gcgtcgcggc atgcggacac gggcgcccgg agcgggcact gctccacccc
     1021 gcggaaaacg cccgtcaccg tgccggagcc gtcggcatga accagacggc cattgccggt
     1081 gtcggccacg atgatctcgc catcaggccc ggcccagacg ccgcgtggcg agcggagacc
     1141 gcccgcacgc tcgaaagcgt actgctccat gaacctcccg cgcgcatcca gcacctggat
     1201 cagatggttc cccgtatcgg ccacgagcag gttgttgaac cggtccatgc acactgcctc
     1261 gggagaccgg aaacgcccga agccctcgcc ccgcccgccg atggagcaca tgtacgtgcc
     1321 atcctgggtg agcttggtga tgcggtggtt gcccatctcg agcacgtaga tgaacaaccg
     1381 gggatctacc accacatcac tcggcatccg gaactgcccg gctccttcgc ccaccccacc
     1441 gtagccgtag gcctcatccc gcggcgtgat cttctggatg cggtggcgca tggtgtcggc
     1501 cacatagagg ttctcggcgc catcgatgca cagccccgag ggtgacgaga aacggccgat
     1561 ctcctgcccg gcctcgccga tggtctcgag caatgccagc cgcccgccgc tggcatggtg
     1621 gcgggccgtg ggggccgtgc tctgcgaaga caagtcgttg atcgatggga gaaggatctc
     1681 ctcctcaacc ggcgcctgtg gggcctcggc gatctccaga aagtcgaacc ggtcgcgacg
     1741 catgcggtcg ccgtcactcc tcgtcttcga catccggcgg gccgacggcc gtggcgcggc
     1801 gctcggcctc gaaggcgcga taggccgcag ccgctgcctc ggccacgccc ggctgcatca
     1861 tgatgccgta gtcctctggc cccagatcgg gcaatggttc ggatggctcg cttgacgacg
     1921 gccgcgcgcg gttctgcgcc cagcggcgca ggaagtcgat ctcggcagcc atggtccggc
     1981 acagtgggaa agtttgctgg acggcggtga cgatatcgtc ggtcgtcacc tcagcgccct
     2041 tgtcaaatgc atcgtacatg cccgcgatca ccgcctgttc gatctccgcg cccgagaagc
     2101 ccgccgagac ggcggcgaga cgctcaagat cgaaccgctc cacgtcgcgg ccacgccggg
     2161 cgaggtgaat gcggaagatc tccttcctct ccgtaatgcc cgggaggtcg acgaagaaga
     2221 tgtcgtcgaa ccggcccttg cggagcaact cgggcggcag ctgtgagatg tcgttggcgg
     2281 tggcgatgac gaacaccggc gctgtcttct cctgcaacca agttagcagt gtgccgaaca
     2341 cccgggcggt agttcccgcg tccgatgagc tgctgctctg cacgccagac aggcccttct
     2401 cgatctcatc gatccacaac acgcatggcg ccacggactc ggcgatgcgg atggccttcc
     2461 gcatgttctc ttctgaggcc cccaccagcc ccgcgaagat gcgccccacg tccatcttca
     2521 gcaacggcag ccgccacagg tgcgcgatgg acttggctgt cagtgacttg ccgcagccct
     2581 gaacccccag gagcagcagt cccttgggct ccggcaggcc aaactgccgt gcggattcct
     2641 cgaacgcacg cgtgcgcttg cccagccagc tcttgaggag gtccagccca cccacgctgc
     2701 ccacatcagt ctcggtgcgc agatactcga gtacctcggt cttgcggatg atctgctcct
     2761 tctccagcag aaccacatcg acgtcgagac acttgcgctc gacaatggac cgcgcgaaca
     2821 cgttctcggc ctcggccagt gtgagcccca aggccgcttt gaggagcttg tcccgttcct
     2881 cgggcgagag atcggtgttg atctgcttat tgcctttcac cgactcgatg atgcggtcca
     2941 gcagcgcccc catgtcctcg aaggtcggca agccgaagtc cacgacggtg atggccttct
     3001 cgagctcggg gggcaagcgg agcactggcg agacgataat gatcgtcttg tagctcatct
     3061 tcaacgcgaa cgtcgcatcc cgcagcttgc gtatgaccgc cgtcgcgtac tgcgggtcgt
     3121 gaaggaacgg atggaaatcc ttgagcacga acacggcctg gtccttcgag gccacgatcc
     3181 ggtcgaggac gaccaacgga tcgcggctct cctcatcgcc tcgaggcgtg ttgaacggac
     3241 gcagaccgtc agtgaccgtc cagatgaaca gcttcttgct ccgtgcccgg gccacggcgg
     3301 cgaggacatc ctcgacccgg gcctcctccc acgagacgac atagacaaga gggtaccggg
     3361 cacgaacgag gatctcgatc tcgcgggctt ggctctccgg catgggttcc ccgctctccc
     3421 gcggcaacgc gtggcagtgc ggcgattata gcgcggaagc gggccgccgc tgtgcccgtg
     3481 gaggaggcag cgtccgcccc catggccgct agaagcgcgt ctgccgcgac tggcagagga
     3541 gcagcaggaa gaatggacac cccagaaccg atgtcacaat gccgacgggc acctcctgga
     3601 agcccggcat cgtccgcgat agcaggtcgg cgatcaacag cagcacggcc cctcccagcg
     3661 ccgccacagg caacaggcga cggtggtccg gacccaccag caggcgcgag atatgcg
//
LOCUS       DAOSPL010000271         3703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_24967_length_3703_cov_3.302357, whole genome shotgun sequence.
ACCESSION   DAOSPL010000271 DAOSPL010000000
VERSION     DAOSPL010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24967_length_3703_cov_3.302357"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1024)
                     /gene="fliP"
                     /locus_tag="PLQ54_06325"
     CDS             complement(191..1024)
                     /gene="fliP"
                     /locus_tag="PLQ54_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOS92910.1"
                     /translation="MTRRAGAWVVLGLMLVSLGAAAWAQTDSRRPSPGATAPGKTPAA
                     VDPANPDTGFRFSLDPKASDDGMTLQLSGPLRVLFLLTLITLIPAVVMMMTSFTRIVI
                     VLGFLRSALGTQQIPPNQVMVGLALFLTAFVMAPTLRDVNEKALKPYLANEIGVEAAI
                     SRGLTPLRVMMYRQTREADIALMVRLAKLDRPKSLADVPTHVLVPAFIISELKTAFEM
                     GFIIYVPFLIIDMVVASTLMSMGMMMLPPVIISMPFKLLLFVLVDGWHVIVRSLVTSF
                     G"
     gene            complement(1024..1926)
                     /locus_tag="PLQ54_06330"
     CDS             complement(1024..1926)
                     /locus_tag="PLQ54_06330"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOS92911.1"
                     /translation="MAAKPDVFSLVGWMHMSAPVVFARSLGALLLVAAFAAPATAQGE
                     GPRDQGKSGATAARSAVAEAEEQLRREAEGQFTIDSSTEPEAKQAEGSRLLRPTKPQP
                     MWAVGLDAAWRLALVIGLICGVAWVLRRLRSPSVSDASTPGLRVVDTVRLSADRALHV
                     VQARGHTFLIGSAPQSINLVAVLDGHTGYEPASDADALPAYSDSRRWPEDRAAAGPED
                     FFDRLVAAERGFELDDLPEPELEPPAPAPPAPPVRRSPALRPSDALERAMGVISSMEG
                     RLDAPSAGRRHARPAGTYDRQGRL"
     gene            complement(2148..3341)
                     /gene="fliN"
                     /locus_tag="PLQ54_06335"
     CDS             complement(2148..3341)
                     /gene="fliN"
                     /locus_tag="PLQ54_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006694623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HOS92912.1"
                     /translation="MSAPDERTGLAATALAPCWERVADKLTGAFGRTVSLVDMVVRPC
                     TPEELAEALPFAVVGCLHRADGGITGDGLVGLSEEQARAVADLMMGGDGVNFGDEFGD
                     LQVSAVAEALNQMADALAQGLGSAVGNPVSIVCEEAATGVLGEQLDGLRRMAGESPLD
                     LVTARVDVDGALSLSFYHILQSTFVDQLTGGASAAGEAVAASSAPGGMPEPAPAEASD
                     ALMSQDEVAALLGRTGGAAAEEEASPLSARATATDSLAASAAMSQDEIASLLAGISTP
                     APPSPAPSAVTATEARPARFEPLGAPPSVSGESGYELILDIPVQVSVELGRATMRVRD
                     ILGLGSGAIVELDKASGEPVDVLAGGRRIATGEVVVVDENFGVRILQILRVSPDSGTS
                     TDSSR"
     gene            complement(3338..>3703)
                     /locus_tag="PLQ54_06340"
     CDS             complement(3338..>3703)
                     /locus_tag="PLQ54_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HOS92913.1"
                     /translation="LCMPCVVLEPIASKLSAQQWLTSTRKPATEQTIAALQARLAQTT
                     AEASVVLGEARLSMRELVHLKPGDVIVLDRSTNDPLVMRIAGRPKFHVRPGLVGKRMA
                     VRVESLIRSTAPDEEEGNP"
BASE COUNT          637 a         1282 c         1246 g          538 t
ORIGIN      
        1 tgtagagggc tgtcagcatc gccttgctgc cgatgcgcag caccataccc tcgtccatga
       61 tcgactcgct cctcgatctg ccaccacgac caggaccgga cttaccgcgg cagacactgg
      121 tgcgccgaca cgaaccatcc ggcccaatgc cggcgagtgt cctcgtccga cgcgggcccg
      181 cctcgcgcgc ctatccgaaa ctcgtgacca gcgaacgtac gatcacatgc cagccgtcga
      241 cgagcacgaa caacaacaac ttgaagggca tcgagatgat caccggcggg agcatcatca
      301 tgcccatgga catcagcgtg ctggcaacca ccatgtcgat gatcaggaac ggcacgtaga
      361 tgatgaagcc catctcaaac gccgtcttga gctcactgat gatgaatgca ggcacgagca
      421 catgggtggg cacatccgcc aacgacttgg gacggtccaa cttggccaga cgcaccataa
      481 gcgcgatgtc ggcttcccgg gtctgccggt acatcatcac gcgaagggga gtaaggccac
      541 ggctgatggc cgcctccacc ccgatctcgt tggccaggta gggcttcaat gccttctcgt
      601 tcacatcccg cagcgtcggg gccatgacga aagcggtcag gaagagcgcc agaccgacca
      661 tgacctggtt gggcgggatc tgctgggtcc ccagggcgct tcggaggaac ccgaggacga
      721 tgacgatgcg agtgaacgag gtcatcatca tgacgaccgc ggggatgagg gtgatcaggg
      781 tgaggaggaa cagcacccgg agcggccccg agagctggag ggtcatccca tcgtcggacg
      841 ccttcgggtc cagggagaag cgaaaacccg tgtccgggtt ggcggggtcc accgcggccg
      901 gcgttttgcc cggggcggtg gccccaggcg atggccgacg ggagtccgtc tgcgcccatg
      961 cagccgcccc caggctcacc agcatgagcc cgagcactac ccacgcgccg gcgcgcctgg
     1021 tcactagagc cgtccttgac gatcgtacgt cccggcgggc cgggcgtggc gccggcctgc
     1081 cgagggcgcg tccaggcgcc cctccatcga tgagatgacc cccatggcgc gctcgagcgc
     1141 atcgctggga cgcagggccg gcgatcgacg gaccgggggc gcaggagggg cgggggcggg
     1201 cggctccagc tctggctccg gcaggtcatc gagctcgaaa ccacgctcgg cggccaccag
     1261 ccggtcgaag aaatcctcgg ggccggccgc cgctcggtcc tcgggccagc ggcgcgaatc
     1321 actgtaggcg ggcagggcat cggcgtcgct ggcaggctcg tagccggtgt ggccgtcgag
     1381 gacggccacg aggttgatag actgcggggc cgagccgatg aggaacgtgt gtcctcgcgc
     1441 ctgaaccacg tgaagcgcac ggtcggccga cagccggacg gtgtcaacca cccggagccc
     1501 cggcgtggac gcgtcggaca ccgacggact cctgagccgc ctcagcaccc aggcgacgcc
     1561 gcagatcagg ccgatgacca gcgccagacg ccaggcggca tccagaccca cagcccacat
     1621 gggctggggc tttgtcgggc ggagcagcct ggatccctca gcctgtttcg cctcgggctc
     1681 ggtgctggaa tcgatggtga actggccctc ggcctcccgg cggagctgct cttccgcttc
     1741 ggccacggcc gaccgggctg ccgtggcgcc cgacttcccc tggtcccggg ggccctcgcc
     1801 ctgggctgtg gctggagcgg caaaggccgc aaccaacagc agggccccga gagaacgcgc
     1861 aaacaccacg ggggcactca tgtgcatcca ccccaccaga ctgaacacgt cgggcttggc
     1921 agccatcgac gaaatgccag agctccgctt cgcgccatct cgcgccgcac gagagatccc
     1981 tgcggggagg gcgagaagtg cggtctcggt cccggcctcg gtccgtcaag cccactggac
     2041 ccgagggcgc ccacctgagg ggccacccag ccagggcgaa tatggcggcc cgttgttgac
     2101 aaacgccgat cgggtctgcc acacgccatt cgcgggactt gccggtccta cctgctcgag
     2161 tcggtacttg tcccggaatc gggtgagacg cggagaatct ggaggatccg caccccgaag
     2221 ttctcatcca ccacaacgac ctcgcccgtg gcgatgcgcc ggcctccggc gagtacgtcc
     2281 acgggttcac ccgacgcttt gtccagctcg acgatggcgc cagaccccag gcccagtatg
     2341 tcccgcactc tcatggtcgc gcggcccaac tcaacactca cctggacagg gatatcgagg
     2401 atgagctcgt accccgactc accgctgacc gaaggagggg cgccgagcgg ttcgaaccgc
     2461 gcgggccgcg cttcggtggc cgtgacggcc gatggcgcgg gcgagggcgg cgcgggtgtg
     2521 gagatgccgg cgagcagcga ggcgatctcg tcctgcgaca tggccgccga cgcggcgagt
     2581 gagtcggtcg ccgttgccct ggcggagagc ggcgaagcct cttcttcggc tgcggcccca
     2641 ccagtccggc cgaggagagc ggccacctcg tcctgactca tgagggcgtc gctcgcctcc
     2701 gcgggggccg gctcgggcat cccaccgggt gcggacgagg cggcgaccgc ctctccggcg
     2761 gcggatgcgc cgccggtcag ctgatcgacg aacgtggact ggaggatgtg atagaaggac
     2821 agcgagagag cgccgtcgac atcgacgcgc gcggtcacaa ggtccaaggg actctccccg
     2881 gccatccggc gcagcccgtc gagctgctcg ccgaggacgc ccgtcgcagc ctcctcgcac
     2941 acgatgctga ccgggttccc gaccgcactg cccagtcctt gtgccaaggc gtcggccatc
     3001 tggttcagcg cctcggccac agccgagacc tgtaggtcgc cgaactcatc gccaaagttg
     3061 accccgtcgc cgcccatcat gaggtcggct acggcccggg cctgctcctc actcagcccg
     3121 acgaggccat caccggtgat cccgccatcg gcccggtgga ggcatcccac cacggcgaac
     3181 ggcagtgcct cggccagctc ctcgggcgtg cacgggcgca ccaccatatc gaccagggag
     3241 accgtgcggc cgaaggcacc ggtgagcttg tcggcgacgc gttcccagca cggagcgagt
     3301 gcggtggcgg ccaggcccgt ccgctcgtcc ggagcgctca tggattccct tcttcctcat
     3361 ccggcgctgt ggatcgaatg agtgattcca cgcgcaccgc catgcgtttc cccaccaaac
     3421 ccggtcggac gtggaacttg ggacgtcctg cgatgcgcat cacgaggggg tcgttggtac
     3481 tccggtccag gacgatcaca tcgcccggtt tgagatgaac gagctcacgc atggacagac
     3541 gggcttcgcc gagtaccacc gaagcctccg ccgtggtctg cgcgagccgc gcctgcagtg
     3601 cggcgatggt ctgctcggtc gccggcttgc gcgtgctggt gagccactgc tgcgcgctca
     3661 gcttcgaggc aatgggctcg agcaccacgc acggcatgca cag
//
LOCUS       DAOSPL010000272         3703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_24968_length_3703_cov_3.236842, whole genome shotgun sequence.
ACCESSION   DAOSPL010000272 DAOSPL010000000
VERSION     DAOSPL010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24968_length_3703_cov_3.236842"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLQ54_06345"
     CDS             <1..323
                     /locus_tag="PLQ54_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOS92914.1"
                     /translation="GTPIYDQLLAEDRITCSDWSKYTMANVVFRPRSMTAEQLGEARA
                     YAYGHFYSLPCIARRMLVWRGGRPKYVMRTAANLSYRRLHRGGRIWSHIPHDGAIAGV
                     PSRA"
     gene            334..1773
                     /locus_tag="PLQ54_06350"
     CDS             334..1773
                     /locus_tag="PLQ54_06350"
                     /inference="COORDINATES: protein motif:HMM:NF040048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HOS92915.1"
                     /translation="MLCAAILMPMMLVDAGAAVRTFSAGPGPHFRINGESTFLIGNRE
                     GFVGGDWNHEYWNQYDPELDYGEYIARMVDAGINWVRANGISMAEGAGRVHAANTRPD
                     APNYPPKPPFTRRPWLRTGPGVAWDGLPKYDLTRFDPRYFERLTDFVDQCNASGIVVE
                     FILFHQHNIYQIPDHYADNPFRPENNINDVELPTGQPTAYPDFYDETKTRLRGLQIAY
                     VDRVLEAIGGRAVIFTIADEYNWTAEWIRGWARHIREFGRARGLDLVVQLGAAHEPTM
                     TLAAVGGVDSFDLSHYAITPQGAVHSVPIREHTRLSNALSDADFELETEHSTLLAMVQ
                     GPGRPCVREGRADRQAFWTALVSGAAGFYYHGFWQYGGEASTNRSIRRVLERLGDLSG
                     LAPHDDLVDSEQGRAWALAEPGKRYLVYVEGTEAITLRTGGAQGLVAQWFDPRGSTFG
                     PETEVGVDPGVRLPVPDRQDWVLLVRPGR"
     gene            1891..>3703
                     /locus_tag="PLQ54_06355"
     CDS             1891..>3703
                     /locus_tag="PLQ54_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOS92916.1"
                     /translation="MVSYLFVAGLALTIWASAAMGASAQLICSYEQLREDWLTQQRVR
                     GVASGGLAPTLEEDALGGCDGVKDGRWGFHTDAEPNPWWQVDLGEPTPIARIDVYNRA
                     DGAAERAARILCWLSDDGQTWREVYRHNGQVFYGYTDGKPLRIALGGEVARYVRLGLE
                     GTSYLHLDEVEVYRPGDGAPNVALGKVATQSSLSQWSAKHYRTLGDPLTPDALADLVR
                     RGLLLADDLAARGVDVSAARGELQAVAEAVRGLPDDTAYADREALLLRTRAATRSLAL
                     ANPLLDFDDLLFAKRVPGSFSHMSDQNYGWWSRPGGGLFVLEGFRTDTPQLRCLTEGF
                     APGTFANPDLSYDGTRVLFAYCRHYADLAAKPDKLDKASIPEDAFYHVYEVGIDGSGL
                     RQLTTGRYDDFDARYLPNGEIVFLSTRRGQFLQCGLESAMATLTAPFPDSFVRCGGDA
                     YRPVSVYTLHVMDADGQRTRAISPFENFEWTPSVAADGRVLYARWDYVDRSNMPYMGL
                     WATTPDGTEPVAVYGNFTPSPHCVFEARPVPGSRKLMFTASAHHCVTGGSVVLLDPAR
                     GVDGTEPMVRLTPEVCFPEAEGWPNTYFANPYPLSEEY"
BASE COUNT          595 a         1252 c         1253 g          603 t
ORIGIN      
        1 ccggcacccc catctacgac cagctcttgg ccgaggaccg tattacctgc agtgactggt
       61 ccaagtacac catggccaac gtggtctttc gcccgcgaag catgaccgcc gagcagctgg
      121 gcgaggcgcg ggcctacgcc tacgggcact tctactcgct gccgtgcatc gcgcgccgga
      181 tgctagtatg gcgtggcggg aggccgaagt acgtgatgcg tacggcggcg aatctgagct
      241 accggcggct ccaccgcggc ggccgcatct ggagccacat tccccacgac ggcgccatcg
      301 ccggagtacc gagcagagcg taggtggttt tcgatgctgt gcgctgcgat cctgatgccg
      361 atgatgctcg tcgatgcggg tgcagcggtg cgcacattct cggcgggccc gggcccccac
      421 ttccgcatca atggcgaatc cacgttcctg atcggcaacc gtgaggggtt cgtcggcggc
      481 gactggaatc acgaatactg gaaccagtac gatcccgagc tggactacgg cgagtacatc
      541 gcccggatgg tcgacgcggg gatcaactgg gtgcgcgcca acggcatctc gatggcggag
      601 ggcgcggggc gcgtccacgc cgccaacacg cggcctgacg ctcccaacta cccacctaag
      661 ccgccgttca cccgaaggcc ttggctccgg acggggccgg gggtcgcctg ggacggcctg
      721 cccaagtacg acctcactcg cttcgacccg cgctacttcg agcggctcac tgacttcgtg
      781 gaccagtgca acgcatcagg catcgtggtg gagttcatcc tcttccacca gcacaacatc
      841 tatcagatac ccgaccacta cgccgacaac ccgttccgcc cggagaacaa catcaacgac
      901 gtcgagctcc ccacgggcca acctaccgca tatcccgact tctacgacga gaccaagacg
      961 cgcctgaggg gacttcagat cgcgtacgtc gatcgggtgc tcgaggccat cggcggccgc
     1021 gcggtcatct tcaccatcgc cgacgagtac aactggacgg ccgagtggat ccgggggtgg
     1081 gcaaggcaca tccgcgagtt cggccgggcg cgcggtctcg atctcgtcgt ccagctcggg
     1141 gccgcccatg agcccaccat gaccctggcc gcggtcggcg gcgtggactc gttcgatctg
     1201 agccactacg ccatcacgcc ccagggcgca gtccactcgg tgccgatacg cgaacacact
     1261 cggctcagca acgcgctgtc cgacgcagac ttcgagctcg agacagaaca ctcgacgctc
     1321 ctcgccatgg tgcagggccc cgggcgcccc tgcgttcgag aggggcgggc cgatcgccag
     1381 gcattctgga ccgcactggt cagcggggcg gcgggcttct actaccatgg cttctggcaa
     1441 tacggcgggg aagccagcac caatcggagc atacgacggg tcctcgagcg tctcggcgat
     1501 ctgtcggggc ttgcacccca cgatgacctg gtcgactcgg agcagggccg agcctgggcc
     1561 ctggccgagc cagggaagcg gtacctggtc tacgtggaag gcaccgaggc aatcacgctc
     1621 cgcaccgggg gcgcccaggg actcgtcgcc cagtggttcg acccccgcgg gagcaccttc
     1681 ggccctgaga ccgaggtcgg cgtagacccg ggagtccggc ttccggtgcc tgaccggcag
     1741 gactgggtgc tgctggtgcg cccggggcgc tgacccgctc tgccccgccg aggggcgcac
     1801 ggtaggtgtt cgtaggggcc gcgagggacg tcccaagccg atggcgaatg ggcggcggga
     1861 acggcgtgtt cgggcggggg gggatgccga gtggtgtctt acctctttgt ggcggggctc
     1921 gctctcacga tatgggcatc ggcggccatg ggcgcgagtg cgcagctgat atgctcatat
     1981 gagcagttgc gcgaggactg gcttacacaa cagcgtgtac gcggcgtggc ttcgggcggt
     2041 ctggccccca cgctcgaaga ggatgcattg ggcggctgtg acggggtcaa ggacggccgt
     2101 tggggcttcc acaccgacgc cgaaccaaac ccgtggtggc aggtggatct cggggaaccc
     2161 accccgatcg cccggatcga cgtctacaac cgcgcggatg gggcggccga gcgggccgcg
     2221 cgcatcctct gctggctctc cgacgacggt cagacctggc gcgaggtcta tcgtcacaac
     2281 ggccaggtgt tctacgggta caccgacggc aagcccctcc gcattgcgct cgggggcgag
     2341 gtcgcccggt acgtgcggct tgggctggag ggcacctcgt acttgcatct cgacgaggtc
     2401 gaggtctacc gaccgggcga cggggcgccc aatgtggccc tgggcaaggt ggcgacgcag
     2461 agcagcttga gccagtggtc ggccaagcac taccgcaccc tcggtgatcc gttgactcct
     2521 gatgcgctgg cggacctggt ccggcggggc ttgctgcttg cagatgacct ggccgctcgc
     2581 ggagtggatg tctcggccgc gcggggcgag cttcaggcag tggctgaggc cgtccgcggc
     2641 ttgccagacg ataccgccta cgccgaccgc gaagcgctgc tgttgcgcac ccgtgcggcc
     2701 acccggtctc tggcactggc caacccgctg ctggatttcg acgacctgct gtttgccaag
     2761 cgcgtgccgg gcagcttcag ccacatgtcc gaccagaact acggatggtg gtcacgccct
     2821 ggcggtgggc tgttcgttct cgaggggttc cggaccgaca caccccagct gaggtgcctg
     2881 accgagggct tcgcgcccgg gacgttcgcc aacccggacc tgtcgtacga cggcacgcgg
     2941 gtgctcttcg cctactgccg acactacgcg gacctggcgg ccaagcccga caagctcgac
     3001 aaggcgagca tcccggagga tgccttctac cacgtctacg aggtcggcat cgacgggtcc
     3061 gggctgcgcc agctcacgac cggccgctac gacgacttcg acgcgcggta tctgccgaac
     3121 ggcgagatcg tgtttctctc gacgcggcgg gggcagttcc tgcagtgcgg cctcgaaagc
     3181 gcgatggcga ccctcaccgc gccattcccg gacagcttcg tccggtgtgg gggcgatgcg
     3241 taccgcccgg tcagcgtcta tacgctgcat gtcatggacg ccgacgggca gcgtacccgg
     3301 gccatctcgc ccttcgagaa cttcgagtgg accccatcgg tcgcggccga tgggcgcgtc
     3361 ttgtatgccc gctgggacta tgtggaccgg agcaacatgc cctacatggg gctgtgggcc
     3421 acgacccccg atggcacgga acccgttgcc gtctacggga acttcacccc cagcccgcac
     3481 tgcgtgttcg aggctcgtcc ggtgcccggg tcgcgcaagc tgatgttcac agcgtcggcc
     3541 caccactgcg tcaccggcgg gagcgtcgtc ctgctcgacc cggcgcgcgg cgttgacggc
     3601 accgagccca tggtgcggct gacgcccgag gtgtgtttcc ccgaggctga gggctggccc
     3661 aacacgtact tcgccaatcc gtacccgctc tcggaggagt act
//
LOCUS       DAOSPL010000273         3695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25041_length_3695_cov_3.131044, whole genome shotgun sequence.
ACCESSION   DAOSPL010000273 DAOSPL010000000
VERSION     DAOSPL010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25041_length_3695_cov_3.131044"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2452)
                     /locus_tag="PLQ54_06360"
     CDS             complement(<1..2452)
                     /locus_tag="PLQ54_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOS92917.1"
                     /translation="MRRLATPIVAAAVFASATPSAYASEWVRVAYDDAGSAGAQPHLT
                     TGTNWRFEGPADVDEALRTVAFGERVTFAYAGMNPAAPYRAILRFCSDGPREMRVWVG
                     ATVAFESVALGAGSVEVREIDLSPVAYAPGSLVLAVERVQGPNAVVADIEILSTDPTP
                     LGVRRLPDVPPPRLTPRPERVLGGPATHLDLNGRWRFHPAPPGDFAEGKPSGSGWSDI
                     TVPGEWAMQGFEVTRETPAAYVRTFRIGSKPAGERWKLRFSAVYSLCRAWVNGVAVGE
                     HEGGFVPFEFDVTEALRPGENTLAVSVQSESMLDRLSCGSQYAAHTLGGITRKVELFA
                     VPEVHVADLAIETRLDLMARDAVLTARVAIRNESNQASVGRAQFTIEPVGRSTRVDAA
                     SAAVAWSGLAPGETRVGPVRIPVRSPSLWDHEHPNLYRLMVRLSGPQGATELVSETFG
                     FREVRRDGTQVLLNGQPIKLRGVCRHEVHPLLGRALTPELWHRDAELYRAGNCNFIRT
                     SHYPPAEEFIAECDRLGLLVELEAPLCWVGHGASSAYTSIPMEDEVFVRLAQANLDTV
                     YGYRNHPSVILRSLANESAWSPLWARVHALVREADPTRLTTFHDQCWGGYNNQGSSEM
                     PVGIIHYPGPGGPAQTEAETRPIQFGEYCHLNSYNRRELVTDPGLRDVWGPGLAAMWE
                     AMWAAPACLGGSIWAAMDDTFFLPSGETVGYGTWGPLDGWRRPKPEYWHMKKAYSPLR
                     LREQVIPLPRAGEPVLLTVENRFAFTDLAEVGFEWTVGERHGRARARGAPGETGRLRI
                     PVGAAKPGELLTLRAFSPL"
     gene            complement(2455..>3695)
                     /locus_tag="PLQ54_06365"
     CDS             complement(2455..>3695)
                     /locus_tag="PLQ54_06365"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOS92918.1"
                     /translation="LGEPHRELVCIHVAGTNGKGSVCAMAESVLRVSGLRTGLFTSPH
                     LIEPRERIRLDGVAVSPEQFAAQVACIRQAVDALPGEAAPTFFELYTALALRLFWQQK
                     VDVAVVEVGMGGRLDSTNVLSPAVSVITSVGLDHAAHLGGTLPQVAREKAGIIKTGVP
                     VVCGISANDPAAQPALEVIRGVSTRCGSELWLRDAEFGGTRRPAGGVDLWVGPRRFDT
                     PVLGLAGTFQAANAATAVASLVRLEDSRAVRVPGWRLRGSDVSAGLTSVQWPGRFQVV
                     RPPGWPVVVLDGAMNAEAASALRQALEDRFPGRRIVFVIAMSADKQPVEFARALFPGL
                     ARALIVTRAQTPRAMPVEDLREAVLCVLGPGVAVGAASTVVEALDAARQASGDDGVVC
                     LTGSLYLVGEALACLDGLEG"
BASE COUNT          606 a         1321 c         1255 g          513 t
ORIGIN      
        1 cgagagggct gaatgcccgg agtgtcagca gctcgcccgg ctttgcggct ccaaccggga
       61 tcctgagcct gccggtctcg ccgggcgctc ctcgggcgcg ggcccggccg tgccgttcgc
      121 ccacggtcca ttcgaagccg acctcggcga gatcggtgaa cgcgaatcgg ttctcaaccg
      181 tcagcagcac gggctcgccg gcccggggca gcgggatgac ctgctcccga agccgcagag
      241 gtgaatacgc ctttttcatg tgccagtact cgggcttcgg ccggcgccag ccgtcgagcg
      301 ggccccaggt gccatagcca acggtctcgc ctgagggcag gaagaacgtg tcatccatgg
      361 ccgcccagat ggacccaccc agacacgccg gcgccgccca catcgcctcc cacatggccg
      421 ccagccctgg tccccacaca tcgcgcagcc ccgggtcggt gaccagctcg cgccggttgt
      481 agctgttgag gtggcagtac tcgccgaact gtatcggccg cgtctcggcc tcagtctgtg
      541 cggggccgcc gggcccaggg tagtgaatga tccccacggg catctccgac gagccctggt
      601 tgttgtagcc tccccagcac tggtcgtgga aggtcgtcag gcgtgtggga tcggcctccc
      661 gcacgagcgc gtgtacccgc gcccagagcg ggctccaggc cgactcgttg gccagcgagc
      721 gcaggatgac cgaagggtgg ttgcggtagc cgtagaccgt gtcaaggttg gcctgggcga
      781 ggcgcacgaa gacctcgtcc tccatcggga tcgacgtgta ggcgctgctc gcgccatggc
      841 cgacccagca caggggcgcc tcgagctcga ccagcaggcc caggcggtcg cactcggcga
      901 tgaactcctc ggccggcggg tagtgagagg tgcggatgaa gttgcagttg cccgcccggt
      961 acagctccgc atccctgtgc cacaactcgg gggtgagcgc ccggccgagc agcgggtgca
     1021 cctcgtgtcg gcacacgcca cggagcttga tgggctgccc gttcagcagg acctgcgtgc
     1081 cgtcgcgccg gacctcccgg aatccgaatg tctcgctcac caactcggtg gcaccctggg
     1141 gcccggacag ccgcaccatc agacgataca ggttcggatg ctcatgatcc cacagagagg
     1201 ggctccgaac cggtatccga accgggccga cccgcgtttc gcccggcgcc aagcccgacc
     1261 acgccaccgc tgccgaagca gcatccaccc gcgtcgatcg cccgaccggc tcgatggtga
     1321 actgggcccg gcccaccgac gcctgattcg actcgttccg gatcgccacc cgcgccgtca
     1381 gaacggcgtc acgcgccatg agatccagcc gtgtctcgat ggcgaggtct gcgacgtgca
     1441 cctcgggcac agcgaacagc tcgaccttgc gcgtgatgcc gcccaacgtg tgcgcggcat
     1501 actgcgagcc gcacgagagg cgatcgagca tcgactcgga ctgcacgctg acggcgaggg
     1561 tgttctcgcc gggccgcagg gcctcggtta catcgaactc gaagggtacg aagccgcctt
     1621 cgtgctcgcc cacggccacg ccgttgaccc acgcgcggca gaggctgtag acggccgaga
     1681 agcggagctt ccaccgttcg cccgccggct tggagccgat gcggaatgtt cgcacatagg
     1741 cggcgggcgt ctcgcgggtc acctcaaagc cctgcatcgc ccactcgccg ggtacggtga
     1801 tatcagacca gcctgaaccc gagggtttgc cttcagcgaa gtcacccggt ggcgcgggat
     1861 ggaagcgcca tcgcccgttg aggtcgagat gcgtggccgg accgcccagc acccgctcgg
     1921 gtctcggagt gagccgcggg ggagggacat cggggagccg tcgcacgccc aggggcgtgg
     1981 ggtcagtcga taggatctcg atgtcggcaa ccacggcgtt cgggccctgc acgcgctcga
     2041 cggccaggac cagcgagccc ggtgcgtacg ccactggact caggtcgatc tctcgcacct
     2101 cgaccgatcc ggcgcccagc gccacggact cgaatgcgac ggtggcaccc acccacacgc
     2161 gcatctcgcg cgggccgtcg gagcagaacc tgagtatggc cctatagggt gcggccgggt
     2221 tcatcccggc gtacgcgaat gtgacgcgct cgccaaacgc gacggtccgc agcgcctcgt
     2281 caacgtccgc cgggccctcg aaccgccagt tggtgcccgt ggtgaggtgg ggctgtgcgc
     2341 cggccgatcc agcgtcgtcg tatgccacgc gcacccactc ggaggcatag gcggatggcg
     2401 ttgccgaggc gaacacagcg gctgccacga tgggggtggc cagacgtctc acgatcatcc
     2461 ctccaggccg tcgagacacg ccaatgcctc gcccaccaga tagagcgagc cggtcaggca
     2521 caccacccca tcatccccgg atgcctggcg tgcggcgtcg agagcctcga cgacggtgct
     2581 cgccgcgcca acggcaacgc cgggccccag cacgcacagc acggcctcgc ggaggtcctc
     2641 caccggcatg gcgcgcggag tctgggctcg cgtgactatc agggcccgtg ccagccctgg
     2701 gaacagcgcc cgcgcgaact cgaccggttg cttgtcggcg ctcatggcga tgacgaagac
     2761 gatccggcgt ccggggaacc ggtcctcaag agcctgacga agggccgaag ccgcctcggc
     2821 gttcatggcg ccatcaagca ccacgacggg ccagccggga ggccgcacca cctggaagcg
     2881 gcccggccac tggaccgagg tcagaccggc actcacgtca gacccgcgta gtctccagcc
     2941 cggtacccgg acggcccggc tgtcctcgag ccgcaccagg ctggccacgg ccgtggccgc
     3001 gttggccgcc tggaaggtgc ccgccaggcc caggaccgga gtatcgaagc ggcgcggccc
     3061 cacccacaga tcgaccccgc cggccggccg gcgcgttccc ccgaactcgg catcacggag
     3121 ccacagctca ctgccgcacc gggtgctgac tcctcggatg acctcgaggg ccggctgcgc
     3181 ggccggatcg ttggccgaga tcccgcacac cacgggcacg cccgtcttga tgatgccggc
     3241 cttctcgcgg gcgacctggg gcagggtgcc gcccaagtgc gcggcgtgat cgaggccgac
     3301 cgacgtgatg acgctgaccg cggggctcag cacattggtg gaatcgagcc ggccgcccat
     3361 gccgacctcg accacagcga cgtcgacctt ctgctgccaa aacagccgca gcgcgagggc
     3421 ggtgtagagc tcgaagaagg tcggcgcagc ctccccgggc aaagcatcca ccgcctggcg
     3481 aatgcacgcc acttgagccg cgaactgctc gggcgagacg gccacgccgt cgaggcgaat
     3541 acgctcccgg ggctcgatca ggtgaggtga cgtgaacagg ccggtgcgga gcccgctgac
     3601 cctcaggacg gactccgcca tggcgcagac cgagcctttg ccgttggtgc ccgcgacatg
     3661 gatgcagacc agctcgcggt ggggctcgcc gaggg
//
LOCUS       DAOSPL010000274         3681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25180_length_3681_cov_3.638445, whole genome shotgun sequence.
ACCESSION   DAOSPL010000274 DAOSPL010000000
VERSION     DAOSPL010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25180_length_3681_cov_3.638445"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PLQ54_06370"
     CDS             <1..795
                     /locus_tag="PLQ54_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92919.1"
                     /translation="LLAGSGELDVLAATRVLAPALGLHTTEAARLVVSSHGVPATELD
                     GVRAQQILMALAAQGVPAALVPTSELVAVPQPHEFCSLNWADAEHLVSANREGEVRRL
                     LWADIVALCVARVEPPVSRHGSLDQVLPTTPLARLMTKSIRAWAESESVPNRPGLSRE
                     PETLLSLVSRDAHDHVEHWRAAAERMSYACLGDRASPHAATNFHAVVTALHARLGGAG
                     TNLTERMIAQPNLFPWPVVDHPSRLDRLLFRLVNLPRPERESRSVR"
     gene            1039..2694
                     /locus_tag="PLQ54_06375"
     CDS             1039..2694
                     /locus_tag="PLQ54_06375"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HOS92920.1"
                     /translation="MRRLLEPYAADWRSLCRFYDVPLSCQRLQRLSRFNDAWRQRLAE
                     VDFAALDGDGQVDYLLLRNHLDHERFLLQLQEERRSEVAGLLPALGEITDLEESRRAM
                     AHPAPELLAQRLSSLADSVEASRRRVGQVDASGPSRNECHRAARSLEQLLPVFDSWHR
                     HYHDFKPEYRWWTGQPFGRARKALRRCLKDLRSRARGGAGEDSPRSLVGDPIGRRALE
                     AALAHEMLPYGPEDLVRLGERELAWCTREMERASAELGYGTNWRHALNHVKERHVAVG
                     QQDLLVARLAREAIDFVTTRDLVTVDDLCRETWYVDMIDEKGQQTLPYQGYGGQRLLA
                     SYPTATMDPAAALTSLRSNNVHFSRAVTHHELIPGHHLQAYVAERSREHRGIFATPFL
                     SEGWCVYWEMLLWDLGYARGPEDRVGMLFWRMHRCARIVVSLRFHLGEMEPPDMVDYL
                     IEHVGHEREAAEGEVRRYISDDYPPLYQCAYLIGALQLRALRRECVDSGRMSDRVFHD
                     AVLRENAIPIELIRSRLLALPLTRDFRPSWRFEASAFEGHDGT"
     gene            2789..3361
                     /locus_tag="PLQ54_06380"
     CDS             2789..3361
                     /locus_tag="PLQ54_06380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOS92921.1"
                     /translation="MGRSDADLLRAVGAGDVSAFSAFVDRHKDRVFSFAVEVIGNRAD
                     AEDASQETFVEAYRFARRFQGEGSAVAWLLGIAWHRCLTWKYRWRRHAVSLDDLPSPE
                     GVVGNGTDRRVDDEAEATAVRLDVRRALAKLPPKYRVPALLRYQQGLSHEEIGAALGI
                     SPATAAQRVHRAKVILRTALHHLDPRAGGG"
     gene            3367..>3681
                     /locus_tag="PLQ54_06385"
     CDS             3367..>3681
                     /locus_tag="PLQ54_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92922.1"
                     /translation="MDCREARRYLGRPSGPEPGTAHWARAEQHAAACPVCRAYVESLQ
                     RIDEILSASLCPHLAPADFTARVVEAVARPHQPRTAPVGLRPALAWALAAERRPLGLL
                     GIR"
BASE COUNT          578 a         1186 c         1300 g          617 t
ORIGIN      
        1 ctgcttgccg gatccggcga gctggacgtg ctggctgcca cacgagtgct ggctccggcg
       61 ttggggttgc acaccaccga agcggcgcgg ctggtggtct cgtcgcacgg cgtacccgcg
      121 acggaactcg atggggtccg ggcccagcag atcttgatgg cactggcggc ccagggcgta
      181 ccggccgcgc tggtgccgac ctcggagctg gtggcggtcc cgcagcccca tgagttctgc
      241 tcgctgaact gggccgatgc cgaacacctc gtatcggcca accgcgaggg cgaggttcgc
      301 cggctgttgt gggcggacat cgtcgcgctg tgcgtcgcgc gtgtcgaacc accggtgagc
      361 cgacacggga gtctcgacca ggtgcttccc accacaccgc tagcccggct gatgacgaag
      421 tccatccgcg cctgggcgga gagcgagagc gtcccgaacc gcccggggct ctcgcgcgaa
      481 cccgagacac ttctgagcct ggtgtcgcgc gacgcccatg accacgtcga gcactggcgt
      541 gccgccgccg aacgcatgtc ttatgcttgc ctgggggacc gcgcctcgcc gcacgcggcc
      601 acgaacttcc acgcggtggt gaccgccctc cacgcacggc tgggcggggc cggcaccaac
      661 ttgaccgagc gcatgatcgc gcaacccaat ctcttcccgt ggccggttgt ggatcacccg
      721 tcacggctcg atcgcctact cttccggctc gtcaacctgc cgaggccgga acgggagagc
      781 cgatccgtcc gctgacactc gaccgggcac cctcggcctc ggctgagcgc cacccggggc
      841 gacggcggta ctcaccgggt atgcgaggac cgccagctca accgactggc actcgatccc
      901 gagtggccga ccacgacggg tgccccgcaa tgcgcgcagc cgcgcatcgg gccggcaaag
      961 gcgatgcgcc gggagttggg gaacacgggg cgatgggggg cggaccgccc gctcgtttcg
     1021 cgccggtggg gaggcgccgt gagacgactt ctggagccct acgccgctga ctggcggagc
     1081 ttgtgccggt tctatgatgt gccactctcg tgccagcgtc tacagcggtt gtcgcgcttc
     1141 aacgacgcgt ggcggcagcg cctcgcggag gtcgatttcg cggctctcga tggcgacggc
     1201 caggttgact atctactgct ccgcaatcat ctggaccatg agcgcttctt gttgcagctc
     1261 caggaggagc gaagaagtga ggtggccggg cttctgcccg ctctcggtga gatcacggat
     1321 ctcgaggaat cgcggcgagc catggcccac ccggcacccg agcttctggc ccaacggttg
     1381 agctcgctgg ctgactcggt cgaggcgtcg cgacggcggg tcgggcaagt cgatgcctcg
     1441 ggcccctcgc gcaacgagtg ccatcgagct gcgcgaagcc tcgagcagct gctccccgtg
     1501 ttcgactcat ggcatcggca ctaccacgat ttcaagcccg agtaccgctg gtggacaggg
     1561 cagccctttg ggcgcgcacg caaggcgttg cggcgctgcc tcaaggacct tcgcagccgc
     1621 gcccgaggcg gtgccggcga agactcgcca agatcactgg tcggggatcc gatcggccgg
     1681 cgggcgcttg aggcggctct ggcgcacgag atgctcccct atggccccga ggacctggtg
     1741 aggttgggcg agcgggagct cgcatggtgc acccgcgaga tggagcgggc atcggccgag
     1801 ctcggctacg ggacgaactg gcgccatgct ctgaaccatg tgaaggagcg tcacgtcgcg
     1861 gtgggccagc aggacttgct cgtggcccgc ctggcgcgcg aggctatcga cttcgtgaca
     1921 actcgcgacc tggtgaccgt cgacgacctg tgtcgggaga cgtggtacgt ggacatgatc
     1981 gacgagaaag gccagcagac gctgccctat cagggctatg gcggccagag gctgctggcg
     2041 tcgtacccca cggccaccat ggacccggcg gcggcgctca ccagcttgcg gagcaacaac
     2101 gttcacttca gccgagcggt cacgcaccat gaactcattc ctggtcatca tctccaagca
     2161 tatgtggccg agcggtcaag agagcaccgt ggcatcttcg ccaccccttt cctcagcgag
     2221 gggtggtgtg tgtactggga gatgttgctc tgggacctgg ggtacgcacg gggacctgaa
     2281 gaccgtgtgg gcatgctgtt ctggcgcatg caccggtgcg cccgcatcgt ggtatcgctt
     2341 cggttccacc tcggtgagat ggaaccacct gacatggtcg attacctgat cgagcatgtg
     2401 ggacacgagc gcgaggccgc tgagggggaa gttcggcggt acatcagcga cgactaccca
     2461 ccgctgtacc agtgtgccta cctgatcggg gcgctgcagc tccgggctct ccggcgcgag
     2521 tgtgtcgact ccgggcgtat gtcggaccgc gtcttccatg acgccgtgct ccgcgagaac
     2581 gccatcccca tcgagctgat ccggtcgcgg ttgttggcac tccccctgac ccgggacttc
     2641 cgcccgagct ggcgattcga ggcaagcgca ttcgagggcc acgatgggac ctaggcgccc
     2701 gccaacagac ctgcctgctc gatctcgtca agaagcggcc gagtccagcg aaatggatta
     2761 tggcagggag cattcagggg ggcagtggat ggggcgctcc gatgccgacc tgctaagggc
     2821 ggttggggct ggcgacgtga gtgccttctc cgccttcgtt gatcggcaca aagaccgggt
     2881 cttcagcttt gccgtcgaag tgatcgggaa ccgagcggat gccgaggatg cgtcgcagga
     2941 gacgtttgtc gaagcctacc ggttcgcccg gcggttccag ggcgagggct cggcggtggc
     3001 gtggctcctg ggcatcgcct ggcatcggtg cctgacctgg aagtaccgct ggcgacggca
     3061 tgccgtctcg ctggatgact tgccgtcgcc cgagggcgtg gtcggtaatg gcaccgaccg
     3121 gcgagtcgac gatgaggctg aggcgacagc ggtgcggctc gacgtgaggc gggcgctggc
     3181 aaagctaccg ccgaagtacc gggtgcccgc gctgttgcgg tatcagcagg ggttgagtca
     3241 tgaggagatc ggtgcggctc tgggtatcag cccggcgacg gcggcccagc gggtccaccg
     3301 agccaaggtc attctgcgca cggcactgca ccatttggac ccacgcgcag ggggagggtg
     3361 atcggcatgg actgccgtga ggcacggcgg tacctcggga ggccatctgg acccgagccg
     3421 ggcactgcgc actgggcccg ggctgaacag catgccgccg cgtgcccggt gtgccgggct
     3481 tatgtggagt cgcttcagcg aatcgatgag atcctctcgg cctcgctatg cccacacttg
     3541 gcaccggctg atttcacggc acgggttgtt gaggcagtag cgcgaccaca ccagccgcga
     3601 acggcgccag tgggcctccg tccggctctg gcgtgggcgc tggcggcgga aaggcgcccg
     3661 cttgggctgc tgggcattcg g
//
LOCUS       DAOSPL010000275         3680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25192_length_3680_cov_3.311724, whole genome shotgun sequence.
ACCESSION   DAOSPL010000275 DAOSPL010000000
VERSION     DAOSPL010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25192_length_3680_cov_3.311724"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..>3336)
                     /locus_tag="PLQ54_06390"
     CDS             complement(260..>3336)
                     /locus_tag="PLQ54_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOS92923.1"
                     /translation="VMSVGRFALTRSRGVLAIAVLLVAPRLWALWHVPLAILPDFPYP
                     RIAVIADAGDVAIETMVQRVTRPLESAAAVVPGVTRVRSRTARGSVEMSVDFDWNEDM
                     FQALTYLRGSIDSIRDDLPSGVSLIVERQDPSSFPVIGYSLTSQTVDQATLRELADLQ
                     VAPTLSRLRGVYRVLVQGGDVREFTVAARPEALAAAGVSIADVTAAIQDTNQVSSVGR
                     FDKDHMKYLVVGTAEMRGVDDVKSAVVATKGDVPIRVRDVADVFESSEERTTAVTANG
                     QPAVLVSVLKQPGASVVAVSDELRESFADLVHSLPAGIEIRPFYDESDLVREAIGSVR
                     DAVILGAVLAAIVLVAFLGSGRASAAVLLTLPITVLITLLLLRVLGETLNLMTLGGLA
                     VGLGLIIDDVVVTVENIHRHVQEGKGSRQAAVDGTSEITRPMIGSSLTTICVFLPLLA
                     VGGITGAFFAPLALTLTVMLLASMVLAITVAPALCARLLRVEAGHDAASGRMYWLVRL
                     VRRGYAPVLRQVLRHRWETVGASILLALATVAIYRGLPTGFMPEMDEGAFILDYWTPD
                     GTSLAETDRQLRIVEQILLSQPEIEAYSRRTGLELGFFATEQNTGDFAVKLRPRSQRQ
                     RSIQEVIEDTRARVAREVPGLLTEFVLIIQDRVADMAGVPEPVEVKVFGSDPVLLQEL
                     APRVSEVVREVPGVVDVSPGITRSGPELVVRIDPARASRAGFTTNDVIDQLSTSMFGR
                     CETFVRRGERTIPIRVTYPPGRRRTEDQVRRIELVSHTGKLVPLSAIADVSEGAGSAE
                     QEREDQKPLIAVTASLEGVDLGTGVRRVQQAVAENIDLPPGYSVHYGGLFETQQQSFR
                     RLLLVFLFGMALVFVVAVCQYEAFAEPMALFFAAAFSEVGVVLALRLTHTPFNASSFT
                     GAIMVFGMVLTNGIVLMDYIRQRTRLGMPLAEAAAEAGRIRVRPVLMTSTIAILALLP
                     LALGIGAGAEMQKPLAIAVIGGLTVSPFFALLLAPALLTVFRWRQFARGPQP"
     assembly_gap    3337..3346
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3347..3663)
                     /locus_tag="PLQ54_06395"
     CDS             complement(<3347..3663)
                     /locus_tag="PLQ54_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HOS92924.1"
                     /translation="MVGADVDTETNTGEVVVWLENAQYVLKEGAFATATIATAGVERV
                     TVPAQALVSEEAEQFVFVADADDVARKVAVTVGRRQGDMAEIVSGLKAGERVVTVGAF
                     GLE"
BASE COUNT          685 a         1315 c         1159 g          511 t
ORIGIN      
        1 tgatgtgaga ccctactcgc tcggctcgcc cgtgaccatg atcgaggagc acatggccga
       61 caccagcacc ggcgacggcc ccgtcgtcct tcaccggcgg gtggtcgagg cccccgaccc
      121 cgtcgagctg ttcatgcgct tctacggcta tccgcggtag ggtaggcgac cgcactgggt
      181 cgcggcgcgg accggcaggt ccgcagccgc cgaagcttgc ccccctcgcg gcccccgtcc
      241 cgcgcgtcac ggggccgacc tatggctgcg gaccccgagc gaactgcctc cagcggaaca
      301 ccgtcagcag cgccggcgcc agcaggaggg caaagaaggg agacaccgtc agacccccaa
      361 tcaccgcgat cgccagcggt ttctgcatct ccgccccggc gccgatgccc agtgccagcg
      421 gcaggagagc gagtatggcg atggtcgagg tcatcaggac gggccggacg cggatcctgc
      481 ccgcctcggc tgcggcctcg gccaggggca tgcccaggcg cgtccgctgc cggatgtagt
      541 ccatcagcac gatgccgttg gtcaggacca ttccgaagac catgatggca ccggtgaaag
      601 aggacgcgtt gaacggggtg tgcgtcaggc gcagggcaag cacaaccccg acctcggaga
      661 acgcggcggc gaagaacaac gccatgggct ccgcgaaggc ttcatattgg caaacggcga
      721 ccacgaacac gagggccatg ccgaacagga acacgaggag caagcggcgg aacgactgct
      781 gctgcgtctc aaacagccca ccatagtgca ccgagtagcc gggcggcaga tcgatgttct
      841 cagcgacagc ttgctggacg cggcgcacac cagtgcccag atccacgccc tccagggatg
      901 ccgtcacggc gatgagcggc ttctggtcct cacgctcctg ctcggccgat ccggcgccct
      961 cggacacatc cgcgatggcc gacagcggaa ccagcttccc cgtgtgcgag accagctcga
     1021 tacgcctcac ctggtcctcg gtgcgccgcc ggcccggagg gtaggtcacc cggatgggaa
     1081 tggtgcgctc gccgcgccgc acgaacgtct cacaccggcc gaacatggac gtcgagagct
     1141 gatcgatgac atcgttggtc gtgaagccgg cccggctcgc tcgagccgga tcgatccgca
     1201 cgacgagctc ggggccgctc ctcgtgatgc cgggagacac atcgacaacg ccggggacct
     1261 cacgaaccac ttcggagacc cttggggcga gctcctgaag aaggaccgga tcgctgccga
     1321 agaccttgac ctcgacgggc tcgggaacgc ctgccatgtc ggccacgcgg tcctggatga
     1381 tgagcacgaa ctcggtgaga agccccggca cctcccgggc gacccgcgcg cgggtgtcct
     1441 cgatgacctc ctggatactt cgctggcgct gagatcgagg cctcagcttc acggcgaagt
     1501 cacccgtgtt ctgctcggtg gcgaagaaac caagctccaa gcccgttcgg cgcgagtagg
     1561 cctcaatctc gggctggctc aagaggatct gttcgacgat ccgaagctgc cggtcggtct
     1621 cggccagcga ggtgccgtcc ggtgtccagt agtccaggat gaaggccccc tcgtccatct
     1681 cgggcatgaa accagtcggc agaccgcggt agatggcgac ggtcgcgagc gccagcagga
     1741 tactcgcccc gaccgtctcc caccgatgcc tgagcacctg gcgcaatacc ggcgcgtagc
     1801 ccctacggac caggcggacg agccagtaca tccgccctga ggccgcgtcg tgccccgcct
     1861 cgacgcgcag cagccgggca cacagcgccg gagcgacggt gatcgccaac accatcgaag
     1921 ccagcagcat gaccgtcaac gtcagagcca agggagcaaa gaacgcgccc gtgatgcctc
     1981 ccacggccag gagcggaaga aacacgcaga tggtcgtcaa cgacgacccg atcatcggcc
     2041 gcgtgatctc actcgtcccg tccacggccg cctggcggct gcccttcccc tcctgaacgt
     2101 gccgatgaat gttctcaacg gtcaccacca cgtcgtcaat gatgagaccc aaccccaccg
     2161 ccaggccgcc cagtgtcatc aggttcagcg tctcgcccag gacacgcagc aacaagagcg
     2221 tgatgagtac ggtgatgggg agggtgagca acactgcggc cgacgcccgc ccgcttccga
     2281 ggaacgccac gagcacgatg gcggccagca cggcgcccag tatgacggca tcacggacac
     2341 tcccgatggc ctcccgcacc aggtcggact cgtcgtagaa aggccggatc tcgattccgg
     2401 cgggaagcga gtggaccaga tcggcgaatg actcccttag ctcgtcagac accgcgacga
     2461 cgctggcccc gggctgcttg agaacactca ccagcacggc cggctgaccg ttggcggtca
     2521 cggcggtggt acgctcctcg ctggactcga agacgtcagc cacgtcccgg acacggatcg
     2581 gaacgtcacc cttggtcgcc accaccgcgg acttcacatc gtcgacgccg cgcatctcgg
     2641 ctgtcccgac gaccaggtac ttcatgtggt ccttgtcgaa gcgccccacg gacgacacct
     2701 gattggtgtc ctggatcgcg gcggtcacgt cggcgatcga gacacccgcc gccgccagcg
     2761 cttcgggcct cgccgccacc gtgaactctc gcacgtcccc gccctgcacc aacacccggt
     2821 acacgccacg aagccggctc agggtcgggg cgacctgcag gtccgccaac tcgcgaagcg
     2881 ttgcctggtc caccgtctgc gaggtcaggc tgtagccgat aacggggaag gacgacgggt
     2941 cctgccgctc cacaatgaga ctgacgcccg acgggagatc gtcgcggatg gagtcaatgc
     3001 tgccgcggag gtaggtcagg gcctggaaca tgtcctcgtt ccagtcgaag tcgaccgaca
     3061 tctccaccga cccgcgggcg gtgcgcgagc gcacacgggt gacccccggc accacggccg
     3121 ccgccgactc gaggggccgc gtcacccgct gcaccatggt ctcgatggcc acatcaccgg
     3181 cgtcagcgat gaccgcaatg cgcgggtacg ggaagtcggg caggatggcc agcggcacgt
     3241 gccacagggc ccagaggcgc ggcgcgacca acaggactgc aatggcgaga accccacgcg
     3301 agcgggtcag ggcgaaccgg ccgacgctca tgactcnnnn nnnnnntcct caagaccaaa
     3361 tgcccccact gtaaccacgc gctctccggc cttcagcccc gagacgatct cggccatatc
     3421 cccctgccgg cgccccaccg tcaccgccac cttgcgcgcc acgtcatcgg cgtccgcgac
     3481 gaacacgaac tgctcagcct cctcggagac cagggcctga gccggcaccg taacgcgttc
     3541 cacccccgcc gtcgcgatgg tggccgtggc gaatgcgccc tccttgagca cgtactgggc
     3601 gttctccagc caaaccacca cttcgccggt attggtctcg gtgtcgacat cggcgccgac
     3661 cacggcgacg cgtccctcga
//
LOCUS       DAOSPL010000276         3676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25221_length_3676_cov_2.929025, whole genome shotgun sequence.
ACCESSION   DAOSPL010000276 DAOSPL010000000
VERSION     DAOSPL010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25221_length_3676_cov_2.929025"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2716
                     /locus_tag="PLQ54_06400"
     CDS             <1..2716
                     /locus_tag="PLQ54_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS92925.1"
                     /translation="LETLEQLFVYDARTDSEPLSFGYALPQFNPPAGELGVPVEPCAT
                     LFCDPTKSVKLAMGMGIFRARLVLLNGSPEKPMGIGFNPLENPRIPLTLHRAAWDVWH
                     LDEVRMKTFRARGVEDPRLNTLHRAAQAELKAADEAREARQWGAVLSHARAGWAFESN
                     AYPDVLKSARDVVSGALFYLALLMSFAYFVERLLISASSVQGRVGWTFGIFLVIFVAL
                     MNVHPAFSLAINPYIILLAFIVLALGLIVTSIVVTKFNEQLEQLKQEVGGIHKADVGR
                     MGAASLAFALGVANMRRRKMRTALTCVTLILLTFTVLSFTSVSSGTKINRIRRAGTPA
                     YYGFMIRDKVWAPLEDSIPAIIDNEFGQTYPVAPRGWVVSGDIAKKAYLTVFSARDPA
                     TEFTVNSILGLSPNERNITDLPKALLPGGRWFEETDGNVCILPQSVAEGLKINPDEVA
                     RWNQRVPERRPGQTDAEYFSSGPFIQFSGTNFRVVGILPDDAIDKFKDLDGETLAPVN
                     YADMKPEIIAQIREMAQQRFKLGRTGVESLLQDYKHFTARELFILPYERLLRVGGTTR
                     SVIMGVRDYAVEQDLIKSFMDRFAISMYAGEVAHGDEPAKVMLYSSIGRQRLDGLETV
                     IIPILIAAMIVLNTMLGAVYERIGEIHIFSAVGLNPTHIGTLFLAESCVYANIGAIVG
                     YLMGQILAKVLFLTGNIEGSGISLNYSSKAAVASTMVVVATVLLSTLWPARRAAEISV
                     PDVDRKWKLPEPDGDNLVLPMPFTVTGLNGRAANMFLVEFFEAYVGYAGGDFYTEGVS
                     FSTIECEHGEGYVVELLMWLAPYDLGVSQHLRIVTEPTGEMNVYAITLHMHREAGDIS
                     SWKKTNWLFLNVFRKQLLIWRTLTPEQREVYAERATEYLSREAETVTA"
     gene            2746..3588
                     /locus_tag="PLQ54_06405"
     CDS             2746..3588
                     /locus_tag="PLQ54_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92926.1"
                     /translation="MHVSLSVESYAASPRVLRGLGELLAGAFPSYSLAIEADIPPHRT
                     VGDLCTTARVLEAAGIPFGLDGPWVQADPGHLRRRRILTVEELGRIHEAAPRQLKWVR
                     IGVGPGSPTTRPVRFADVLGFCRDAGLGAQVVVSEVDWGPGGWARLARSRGPSAVTAL
                     HGLAVASPMNPPRFLVTGCRRGDLTAWGYSLELGRGFPYGEADLHAVLDVIERNMRAG
                     ATLLNVYPPPALVDGLVALPPHLHAWPGIDADALLRPGHGLRLTELGQWLAEHAASGN
                     GAGA"
BASE COUNT          604 a         1249 c         1165 g          658 t
ORIGIN      
        1 cctggagacg ctcgagcagc tcttcgtgta tgacgcgcgc acggattccg aaccactctc
       61 ctttgggtac gctctgcccc agttcaaccc gcctgccggt gagctcggcg tcccggtcga
      121 gccgtgcgcc acgctgtttt gtgacccaac gaagtccgtg aagttggcca tgggcatggg
      181 gatcttccgc gcgcgtctgg tgctgctgaa tggttcgccc gagaagccca tggggatcgg
      241 cttcaacccc ctcgagaacc ctcgcatccc tctcacgctg caccgtgcgg cgtgggatgt
      301 gtggcatctc gacgaggtgc ggatgaagac gttccgggcc cgaggcgtcg aggatccgcg
      361 cctgaacacg ctccacagag ccgcccaggc cgagctcaag gctgccgatg aggcgcgcga
      421 ggcgcgccag tggggggctg tgctctcgca tgcgagagcc ggatgggcct tcgagtccaa
      481 cgcctatccc gacgtgctca agtccgcccg tgacgtggtc agcggtgcgc tgttctacct
      541 ggcactactc atgagcttcg cctatttcgt cgagcgtctg ctgatctcgg cgagctcggt
      601 ccagggccga gtcgggtgga cattcggcat cttcctggtc attttcgtcg ctttgatgaa
      661 cgtccacccg gccttcagcc tggccatcaa tccctacatc atcctcctgg ccttcatcgt
      721 gctggctctc gggctcatcg tgacatccat cgtcgtcacc aagttcaacg agcagctcga
      781 acagctcaag caggaggtgg gcgggattca caaggccgac gtcggccgta tgggtgcggc
      841 gagcctggcc ttcgccctcg gcgtggccaa catgcgccgc cgcaagatgc gcacggcgct
      901 aacgtgtgtc accctcatcc tgctcacctt caccgtgctc tccttcacat cggtgagctc
      961 gggcaccaag atcaaccgga tccgccgtgc cggcacgccg gcctactatg gcttcatgat
     1021 ccgcgacaag gtctgggcgc cgctcgagga ctcgatcccc gccatcatcg acaacgagtt
     1081 cggtcagacc taccctgtgg caccgcgcgg gtgggtggtg tcgggcgaca tcgccaagaa
     1141 ggcatatctg acggtgttct cggctcgcga tccggccacc gagttcaccg tgaactccat
     1201 cctcgggctc tcaccgaacg aacgcaacat caccgacttg ccgaaggctc tgctgccggg
     1261 cggcagatgg ttcgaggaga ccgacggcaa tgtctgcatc cttccgcaga gcgtggcgga
     1321 gggcctgaag atcaacccgg atgaagtggc gcggtggaac cagcgcgtac ccgaacgccg
     1381 ccccggccag accgacgctg agtacttttc gagcgggccc ttcatccagt tctcaggcac
     1441 caacttccga gtagtcggca ttctgccgga cgacgccatc gacaagttca aggacctgga
     1501 tggtgagacg ctggctcccg tgaactatgc ggacatgaag cccgagatca tcgcccagat
     1561 ccgcgagatg gctcagcagc gcttcaaact cgggcgcacc ggcgtcgaat cgctcctgca
     1621 ggactacaag cacttcacgg cccgggagct gttcatccta ccctatgagc gtctgcttcg
     1681 ggttgggggc accacgcggt cggtcatcat gggggtgcgc gattacgccg tcgagcaaga
     1741 cctcatcaag tccttcatgg atcggtttgc catcagcatg tacgcgggtg aggttgccca
     1801 tggggacgag ccagccaagg tcatgctcta cagctccatt ggacgccagc ggctggacgg
     1861 gctcgagacg gtgatcatcc ccatcctcat cgccgccatg atcgtgctca acaccatgct
     1921 cggagcggtc tacgagcgta tcggcgagat ccacatcttc agcgccgtgg ggctcaaccc
     1981 cacgcacatc ggcaccctgt tcctggccga gtcctgcgtc tacgccaaca tcggagccat
     2041 tgtcggctat ctcatgggac agatcctggc caaggtgctg ttcctgacgg gcaacatcga
     2101 gggcagtggc atctcgctga actactcctc caaggcagcc gtggcttcga cgatggtcgt
     2161 ggtggcgaca gtgctcctgt cgaccctctg gcccgcgcgc cgggccgccg agatctcggt
     2221 gcccgacgtc gaccgcaagt ggaagctgcc cgagcccgat ggcgacaacc tggtgctgcc
     2281 catgcccttc acggtgaccg ggctcaatgg ccgggccgcc aacatgttcc tcgtcgagtt
     2341 cttcgaggcg tacgtcggct atgccggcgg cgacttctac accgagggcg tgagcttctc
     2401 gaccatcgag tgcgaacacg gtgagggcta tgtcgtcgag ctgctgatgt ggctggcgcc
     2461 ttacgacctc ggagtaagcc aacaccttcg cattgttacc gagccgacgg gtgagatgaa
     2521 cgtgtacgcc atcaccctac acatgcaccg cgaagccggg gacatcagct cgtggaagaa
     2581 gaccaactgg ctgttcctca acgtgttccg caagcaactt ctcatctggc gcacgctcac
     2641 gcccgagcag cgtgaggtat acgcggagcg ggccacggag tatctcagcc gcgaggccga
     2701 gaccgtcacg gcctagcgta gcgcctggag tcacgggccg gcacgatgca cgtctcgctg
     2761 agcgtggaga gctatgcggc gagtccccga gtgctccgtg gcctgggcga gctcctggcg
     2821 ggggcgttcc cgtcatacag cctggccatc gaggccgaca tcccacctca ccgcaccgtg
     2881 ggggacctct gcacgacggc ccgcgtgctg gaggccgcgg gcatcccatt tggcctcgac
     2941 gggccctggg tccaagcgga ccccgggcat ctgcggcggc gacgcattct gacggttgag
     3001 gagctggggc gcattcacga ggccgcaccg cgccagctca agtgggtgcg catcggggtc
     3061 ggaccaggca gtcccaccac ccggccggta cgcttcgccg acgttctcgg attctgccgc
     3121 gacgccggac tgggcgccca ggtggtcgtc agcgaggtcg actggggccc gggcggatgg
     3181 gcgcggctcg cccggtcgcg aggtccctct gccgtcacgg ctcttcacgg gctcgccgtg
     3241 gcgagcccga tgaacccgcc ccgttttctg gtgactggct gccgccgcgg agacctgacg
     3301 gcctggggct actcgctgga gctgggacgc ggcttcccct atggcgaagc cgacctgcat
     3361 gccgtcctcg acgtcatcga gcgcaacatg agagctggcg cgacactact caacgtctac
     3421 ccgccgcccg cactggtcga cggcctcgtg gcgctcccgc ctcacctgca tgcgtggccc
     3481 ggcatcgacg ccgatgcgct cctgcgccct gggcacgggc tccggctgac tgagctcggc
     3541 cagtggctgg ccgaacacgc ggcatcgggc aacggggcgg gcgcgtaggc gtcccgtgcc
     3601 gcggcccacg ccccacttca cggagctcgt cgcacgccga cccgccgggc ctactgcgct
     3661 accggcgtgg ccgtcg
//
LOCUS       DAOSPL010000277         3672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25261_length_3672_cov_3.518385, whole genome shotgun sequence.
ACCESSION   DAOSPL010000277 DAOSPL010000000
VERSION     DAOSPL010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25261_length_3672_cov_3.518385"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..2399)
                     /locus_tag="PLQ54_06410"
     CDS             complement(249..2399)
                     /locus_tag="PLQ54_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92927.1"
                     /translation="MNRVRVQWWLGRRLLRAPSQRIGLLTVFLVSVLAGLLLVSLCAV
                     PGTLAAARHAEADRTVVNTGSPTGVAASTAIWDWRDEQSVTTVRVGFQGASEAPRVRL
                     PGVPNLPQNGTVWLSPQLMTDIRTDPDLERAFAGRVAGLISEDALTSSQERLAYVGVP
                     VDRLTASGVPLTSAFGAQEPETRGTPLTTSALVLLTVLLFLGVPISALFVGTYGALMA
                     TRSRQIALLRSLGLTPARAQVTASADGVLVGLVGATLSWVCFEVLTSVLTSLPFTPAQ
                     WTAGRPSITPLGAVLSLMTPLLAAGWAARRSVSSPTSRRSNVRVQTRLKADHLWVAVL
                     AASVVAVLASSLLRVDQSPWATAIVVSALTLLIVSAPKAVMALQRQMGRRLLRRGNNG
                     SALIVGARLARTVPNGARTMAMLLPALLLALAVAPLLSQMAPRDSLTAETNQRLGRVV
                     ALVSPVPDPSAREHIRALPGVLAVSAPSEGPNPRYRFDCQAFKTLLAQRPCDQPLDAS
                     QMNGDLSAGWPYLGSWTFTTDPTTRPATDPDQEVPAIVLMRNTDTYWQAVAAIQGLDP
                     LLSVSGNIGGLIGGPDQQYRLLQRWLEVGVALMLITGGGLLVLGVTTDVSESMRLRRG
                     LSALGATPRTLSRHVGGEVLARSLVVLGLAVTSGLAVVVASHGWSNTDTVALPWAAAA
                     LATLATSLMASTGAAALLAARPQIAEHSAADAYR"
     gene            complement(2431..3036)
                     /locus_tag="PLQ54_06415"
     CDS             complement(2431..3036)
                     /locus_tag="PLQ54_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS92928.1"
                     /translation="MRVQRDGRTVFEDVSLAVSAASSLAVTGPSGVGKTTLLDVIAGI
                     LAPDEGQIVFNGFKVTSATRQQAAAWRLRHVGVVHQFAELLPELSLAENAQLPLLLAG
                     FGAQESCRRAEWALDAVGIGALAGAFPSQVSGGERQRAAIARAVVHRPALILADEPTG
                     ALDVQNAHTAARVLIDATASVGATLVMATHNLRSRTFLSAD"
     gene            complement(3130..3405)
                     /locus_tag="PLQ54_06420"
     CDS             complement(3130..3405)
                     /locus_tag="PLQ54_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92929.1"
                     /translation="MAYDKDSNRLTGRDFKDGDYMYIQYAFTNTQCGTTGNACAPPRY
                     STFRLQKPDKDYSEGINVSPTATYILLKLCNDDTPPDTCSNWKRTTA"
BASE COUNT          652 a         1186 c         1238 g          596 t
ORIGIN      
        1 atcaacgtgg tgcagcatcg cgaataggca cgcggcgcga ggcctgtccc cttgctggct
       61 atgtcaaggc aaactggccc cacggtgacg gcctgatctg gccccaagtg agggcgcccc
      121 cagcgctact gcctacgctt tcggcgccaa gttatcggcg gaaatcaggg ctgctgcata
      181 ggtcagtgac agggtaggcc gtaggacccc gcggggccgg cgtgatcgcg accgctgatc
      241 gctcgtcctt agcggtaggc gtccgccgct gagtgttccg caatctgggg acgagcggcc
      301 aggagcgcag cggctcctgt cgatgccatc agtgatgtgg caagggttgc aagtgcagct
      361 gcggcccatg ggagggccac ggtgtcggtg ttgctccacc cgtgtgatgc gacgacgact
      421 gccaagccgc tggtgacggc aagtcccagg acgacgaggg accgtgccag gacttcacct
      481 ccgacatgtc gggacagggt tcgtggcgtc gctccgagag cgctgagtcc tcggcgtagg
      541 cgcatcgact cgctgacatc ggtcgtgacg ccgagcacga gcagtccacc gccggtgatc
      601 agcatgagcg cgaccccgac ctcgagccat cgttggagca gtcggtactg ctggtcaggc
      661 ccgccgatga ggccaccaat gttgccgctg acgctgagca gcggatcgag accttggatg
      721 gcggcgacag cctgccagta ggtatcggtg ttcctcatga ggacgatggc tggaacctcc
      781 tgatcgggat ccgtcgccgg tcgggtcgtc gggtccgtgg tgaaggtcca cgagccgaga
      841 tacggccaac ccgcagagag atcgccattc atctgggagg catcgagggg ctggtcgcat
      901 ggccgttggg cgagtagggt cttgaaggct tggcagtcga accggtatct ggggttgggt
      961 ccttcgctcg gtgctgaaac ggcgaggaca ccaggcaggg cgcggatgtg ctcgcgcgcg
     1021 gaggggtcgg gaacgggcga taccagagcc acaactcgtc ccagccgctg gttggtctcg
     1081 gccgtgaggc tgtcccgtgg agccatctgg gacaggagtg gtgctactgc tagggcgagc
     1141 aggagtgcgg gcagcagcat cgccatggtc cgggctccgt tcgggacggt tcgagccagg
     1201 cgtgctccga cgatcagtgc ggacccgttg ttgcccctcc tcaagaggcg tctacccatc
     1261 tgtcgctgca acgccatgac tgctttcggc gcgctcacga tgaggagcgt caaggcgctg
     1321 accacgattg cagtcgccca cggtgactgg tcgacacgca gtagggagga tgccagaacg
     1381 gcgacgacgc tcgcagccag cactgctacc cacaggtggt ctgctttgag gcgggtctgc
     1441 acccggacgt tgctacggcg gctggtgggt gagctgaccg aacggcgagc cgcccagcct
     1501 gccgcaagaa gcggcgtcat gaggctgagc accgcaccca ggggtgtgat cgacggccgt
     1561 cctgcggtcc attgggcggg tgtgaacgga agcgacgtga gcaccgaggt caggacctcg
     1621 aagcaaaccc acgaaagggt ggcacccacc agtcccacga gcactccgtc tgctgatgcg
     1681 gtcacctgag cgcgggcggg cgtcagcccc aacgacctga gcagagcgat ctgtcgcgag
     1741 cgtgtcgcca tgagcgctcc ataggtgccc acgaacaagg ccgaaatcgg aactccgagg
     1801 aacagcagga ctgtgagcag cacgagggcg ctcgtcgtga gcggtgttcc ccgggtctcg
     1861 ggctcctgcg caccgaaagc agaggtcagt ggcacccctg acgcggtcag ccggtcaacc
     1921 gggacgccga cataggccag tcgctcctgg ctcgaagtca gggcgtcttc gctgatcagg
     1981 cccgctacgc gcccggcaaa agcgcgctcc aagtccgggt cggtcctgat gtcggtcatg
     2041 agctgcggtg aaagccaaac ggtcccgttc tgcggcaggt tgggcactcc gggaagccgg
     2101 acgcgggggg cttcgctggc cccttggaac ccgacccgca ccgtggtcac gctctgctcg
     2161 tcccgccagt cccagatcgc ggtggaggcc gccactcccg tcggtgaacc ggtgttgacg
     2221 acggtgcgat cagcctccgc gtgacgggcc gctgccaatg tgccgggaac cgcacacaaa
     2281 ctcaccagca gcagacccgc aagcaccgac acgaggaaca cggtcagcag cccgatccgt
     2341 tgactgggcg cccgcaacaa ccgtcgccct agccaccact ggacgcggac acggttcacg
     2401 aggcgaccac ccgagacgcg tcggccaggt ctagtcggca ctcaagaagg tccgcgatct
     2461 aaggttgtgc gttgccatga caagcgtggc acccacagag gccgtcgcgt cgatcaggac
     2521 ccgcgccgcc gtgtgcgcgt tctggacatc cagggcaccg gtcggctcgt cggcgaggat
     2581 cagggctggg cggtgtacca ccgcccgagc gatggccgcc cgttgacgct ccccacccga
     2641 cacctgagac ggaaacgctc cagcaagggc gccgatcccg acggcgtcga gagcccattc
     2701 cgcacgacga caagactcct gggctccaaa gcccgcgagc aggagcggca gctgcgcgtt
     2761 ctcggccaac gacagttcgg gaagcagctc cgcgaactga tgcacgacgc ctacatgccg
     2821 gagccgccac gcagcggcct gttgccgtgt ggcggatgtc accttgaagc cgttgaagac
     2881 gatttgcccc tcatcagggg ccaggatccc cgcgattacg tccagaagcg tggtcttgcc
     2941 gacaccgctc ggaccggtca cggcgaggct cgacgcggcc gacaccgcga gactgacgtc
     3001 ctcaaagacg gtgcgaccgt ctcgctgaac gcgcaaccca gagacctcca ggccggctga
     3061 ctccatacga tctccctcgt aacacgctgg tagccaggcc cgcttaagcg ggcccggcca
     3121 ccagtcgact caggccgtcg tccgcttcca gttcgagcag gtgtccggcg gtgtgtcgtc
     3181 gttgcacagc ttgagcagga tgtacgtcgc ggtcggtgac acgttgatcc cctccgagta
     3241 gtccttgtcg ggcttctgca ggcggaacgt gctgtatcgc ggtggcgcgc acgcgttccc
     3301 cgtggtcccg cactgggtgt tggtgaaggc gtactggatg tacatgtagt cgccgtcctt
     3361 gaagtcccta ccggtcaggc gattgctgtc cttgtcgtag gccatctggc cgttgcccga
     3421 aagcgtgtag ctgaatgact acgcggcgaa cgcacccacg gtgctcatcc acgcgatgcc
     3481 gatcgcgacc atgctggcca cgacggcgcg agtagctgac ttccccattg aagctcctct
     3541 gtctggttcc ggtgaaggag gtgcgcccgg atgcgcgagg cccccgtcac ggaagttaga
     3601 cccaagccgt gcacgccgcg agttgaacga agccgccccc accgcgggac ttccgtagac
     3661 cctaccctca gg
//
LOCUS       DAOSPL010000278         3670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25274_length_3670_cov_3.380360, whole genome shotgun sequence.
ACCESSION   DAOSPL010000278 DAOSPL010000000
VERSION     DAOSPL010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25274_length_3670_cov_3.380360"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PLQ54_06425"
     CDS             complement(<1..406)
                     /locus_tag="PLQ54_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOS92930.1"
                     /translation="MGPHEPEESLPRTVALMFTDIEGSVARWESGGKRFAQALALHNA
                     VVRRVVGQHDGFEVRFQGDGFMCAFCRASDALRCAVALGPALAAERWPEGVGVPAIRV
                     AIHAGEVDVVDPRAEFRGVVVAQVRGIELLQVR"
     gene            complement(575..1183)
                     /locus_tag="PLQ54_06430"
     CDS             complement(575..1183)
                     /locus_tag="PLQ54_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOS92931.1"
                     /translation="MNFDATRGRRRYQPPGRPSPFHRPIRRSPFEREGEPVADGAEER
                     PAPDHGFVKGTIADSALFIGLSEGERDDIAAFAEPLVFAPGTCIVREGEPGDALFLLA
                     EGTVEIVTGEGTPHQKVVARLSGGSSLQAGYAGAFFGEMSLIDVEPRSATVRAQTDVK
                     LVRLPAPALRQYYQTHRDAHLAMVSNLARSLSRRLRLLTESV"
     gene            1341..2129
                     /locus_tag="PLQ54_06435"
     CDS             1341..2129
                     /locus_tag="PLQ54_06435"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOS92932.1"
                     /translation="MGRTVIGVTLASALLAAALPCLGGDGGMASAARARAVAASARAY
                     TEGTPARAESARAAARWAFRAATEFLSSDDRTAERWKPIEEALARASEVCGPRFSVAD
                     VPYVRQTDPSLCAIACALMGLRFVGVPATEDALLEMAGPRARVDGVHVSRLADFVAHY
                     GARAWIGEGSEDLVRCSIAIGRPVLVYQYAALDNDVEHMRVVTGFDDRREVYQCWDPA
                     PDLGPSREIPYADFGTLWSIPFREDGASHWLCIIYRGGPGATGP"
     gene            complement(2170..2568)
                     /locus_tag="PLQ54_06440"
     CDS             complement(2170..2568)
                     /locus_tag="PLQ54_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92933.1"
                     /translation="MLESLFGNLWFWIFGVIVGGSLLAKMVRTVADHRLAMARLEAEE
                     RARLRQQELETERRLLGVDDDQNTLGLKLRIEALEKDVTQLRQWVEMVYRALTDGRER
                     SEHEMPQMPRRVTVQANEAAHEAAEHQVTS"
     gene            complement(2619..2966)
                     /locus_tag="PLQ54_06445"
     CDS             complement(2619..2966)
                     /locus_tag="PLQ54_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92934.1"
                     /translation="MVHDFMELLDSPVFWIFGGGTVIALVAIVVDGIIKSQKLRAQER
                     EQERERERESNRALLGLTESDDMAGLKLRIEALEQDVARLEKLLEGPAPRRLNSASAS
                     APAGVEPERLVDA"
     gene            complement(3020..>3670)
                     /locus_tag="PLQ54_06450"
     CDS             complement(3020..>3670)
                     /locus_tag="PLQ54_06450"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOS92935.1"
                     /translation="TFETPYATIERPAHGDEEPGQQWLDLSGVAASTSQPYGAALLNT
                     CKYGFDVLGTEARISVLRSPIYAFHEPKEIDPNETYRYIDQGLQSFTWALLPHTGTWR
                     ESGVVERARELNAVPLVYLDTPHEGEWLAESANVSVEPAHVHVTVLKRAEDGDGVIVR
                     LVETRGQAASARLGLGFLDQTWEGSIGAWEIKTLLFPGPGADGDAVETDLLERPLS"
BASE COUNT          542 a         1382 c         1179 g          567 t
ORIGIN      
        1 cacgtacctg gagcagctcg atccctcgta cttgcgctac tactacgcct cgaaactcag
       61 cccgcgggtc gacgacatcg acctcacccg catggatcgc cacgcggatc gcgggcacac
      121 cgactccctc cggccacctc tccgccgcca gcgccggacc cagagccacc gcacaccgca
      181 gcgcatccga cgcgcggcag aacgcgcaca tgaaaccgtc gccctggaac cgcacctcga
      241 aaccgtcgtg ctgccccacg acccggcgca ccaccgcgtt gtgcagcgcc agtgcctgcg
      301 cgaagcgctt gcccccggac tcccaccggg ccacagaacc ctcgatgtcc gtgaacatca
      361 gcgcaaccgt gcgggggagt gactcttccg gctcatgagg acccatagga gcgcccttcc
      421 gtgcaacccc actcgacgcc gacccgctgc gccatcctgt tcgcagtggg gcctccgaac
      481 gcctccgaac ggaacccgcg cacacaccag cgcccatccc acatcgcgcg gaggggggca
      541 tgggcgcccg cggccggcgt tggccgggac ggtcctagac cgactcggtc aggagccgga
      601 gccgcctgga gagactgcgg gccagattgc tcaccatggc caggtgggca tcgcggtgcg
      661 tctggtagta ctgccgcaga gccggagccg gcaggcgaac cagtttcacg tcagtctgcg
      721 cgcgcacggt ggcgctccgg ggttccacgt cgatgagcga catctcgcca aagaaggcgc
      781 cggcatagcc ggcctggagg gagctgcccc cactgaggcg cgccaccacc ttctgatggg
      841 gcgtcccctc gccggtcacg atctcgaccg tgccttcggc cagcaggaac agggcatcgc
      901 ccggctcgcc ctcgcggacg atacacgtgc caggcgcgaa caccaagggc tcggcgaatg
      961 cggcgatgtc gtcccgctct ccttcgctga ggccgatgaa gagagccgag tccgcgatgg
     1021 tgcccttcac gaacccgtga tccggggccg ggcgctcttc ggccccatcg gcgaccggct
     1081 cgccctcccg ttcgaagggc gaccgccgga tcggccggtg gaatggggaa gggcggcccg
     1141 ggggctgata gcgtcgacgg ccgcgcgtcg catcgaagtt caacgtcgcc acctcgctct
     1201 gtgcccccgc aagccccggg acaggctcct cgatcgtcgg gacccacacc ggattcggca
     1261 cgcccttccc cgttgcctcc ccgcacacct cgctcccggc gcgcgcccgg ctataatgcc
     1321 cgtgtgaggg gggacgggcg ttgggtcgca cggtcatcgg ggtcacgctc gcgagcgcac
     1381 tgctcgccgc ggcccttccg tgcctcgggg gcgatggcgg gatggcctca gccgctcgag
     1441 cgcgtgccgt cgcggcctcg gcccgggcct acaccgaggg cacccccgcc cgcgccgagt
     1501 ccgcgcgggc ggcggctcga tgggccttcc gggccgctac cgagttcctg tcctcagacg
     1561 atcgcacggc ggagcgctgg aagcccatcg aggaggcgct ggcgcgcgcc agtgaggtct
     1621 gcggcccgcg gttctcggtg gccgacgtgc cctatgtccg ccagacggac ccatccctgt
     1681 gcgccattgc ctgcgcgctg atgggcctgc ggttcgtggg ggtacccgcc accgaggacg
     1741 cgctgctgga gatggccggg ccccgcgcgc gcgtcgatgg cgttcatgtg agccgtctgg
     1801 cggacttcgt ggcccactac ggggctcgcg catggatcgg ggagggctcc gaggacctgg
     1861 tgcgatgcag catcgccatc ggccggcccg ttctcgtcta ccagtacgcc gcgctcgaca
     1921 acgacgtcga acacatgcgc gtggtcaccg gcttcgacga ccggcgcgag gtctaccagt
     1981 gctgggaccc ggcccccgat ctgggcccca gccgcgagat cccctacgcc gacttcggca
     2041 cactctggtc catcccgttc cgcgaggacg gcgcctcgca ttggctgtgc atcatctacc
     2101 gagggggccc aggcgcgacg gggccgtagg ggtgagtcct ccaccccgca aaccgcagcc
     2161 ggccgcccgc tacgaggtca cctgatgctc ggccgcctcg tgcgcggcct cgttggcctg
     2221 aacggtcacg cgccggggca tctggggcat ctcgtgctcg gatcgctccc ggccatcggt
     2281 caacgctcgg tagaccatct cgacccactg tcggagctgg gttacgtcct tctcgagcgc
     2341 ttcgatccgc agcttcagtc ccagggtgtt ctgatcgtcg tccaccccga ggaggcggcg
     2401 ctccgtctcg agctcctgct ggcgcaggcg ggcacgctcc tccgcctcga gccgtgccat
     2461 ggccagacgg tggtcggcca ccgttcgaac catcttggca agcagtgagc cgccgacgat
     2521 cacgccgaat atccagaacc acaagttccc gaagagactc tccagcacgc ccatgtgacc
     2581 tccgcctctc cacggtacgg acaggcctcg cggtccgatc acgcgtcgac aagccgctcc
     2641 ggctcgaccc cagccggtgc cgaggctgag gccgagttga gacgccgcgg cgctggcccc
     2701 tcgagcagct tctcgagcct cgccacatcc tgctccaggg cctcgatccg gagcttcagc
     2761 cccgccatgt cgtccgactc cgtcagtccc aggagggccc gattcgactc gcgctcgcgc
     2821 tcgcgctcct gctcccgctc ctgggcacgc agcttctggc tcttgatgat gccgtccacc
     2881 acgatggcca ccagcgcgat gaccgttccg cccccgaaga tccagaagac cgggctgtcc
     2941 agcagctcca tgaagtcgtg caccatgctg actgacctcg ctcccccaga cgggcatggg
     3001 cgactggcgg catctgtccc tagctcagcg ggcgctccag caggtcggtc tcgacggcat
     3061 cgccgtccgc gccggggccg gggaagagga gcgtcttgat ctcccaggcc ccgatggagc
     3121 cctcccaggt ctggtccagg aaccccagcc ccagtcgggc cgatgcagcc tgcccgcgtg
     3181 tctccaccag ccggacgatg accccatcgc catcctcggc ccgcttcagc accgtcacgt
     3241 gcacgtgggc aggctccacg ctcacattcg cgctctcggc gagccattcc ccctcatggg
     3301 gggtatccag gtacaccaaa ggcacggcgt tgagctcccg cgcccgttcg accaccccgc
     3361 tctcgcgcca ggtgcccgtg tggggcagca gtgcccacgt gaagctctgc agtccctggt
     3421 cgatgtaccg gtaggtctcg tttgggtcga tctccttggg ctcatggaac gcgtagatcg
     3481 ggctacggag cacactgatg cgtgcctcgg tgcccagcac atcgaacccg tacttgcatg
     3541 tgttcagcag cgccgcacca tagggctgcg aggtgctcgc cgcgacgccc gacaggtcga
     3601 gccactgctg gccgggctcc tcgtcgccgt gggcgggccg ctcgatggtc gcgtagggcg
     3661 tctcgaaagt
//
LOCUS       DAOSPL010000279         3669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25284_length_3669_cov_3.438849, whole genome shotgun sequence.
ACCESSION   DAOSPL010000279 DAOSPL010000000
VERSION     DAOSPL010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25284_length_3669_cov_3.438849"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PLQ54_06455"
     CDS             <1..812
                     /locus_tag="PLQ54_06455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS92936.1"
                     /translation="LGHWDPQATWHFYLGISDEIESGHLDSGEREHMARYYAEAGLLR
                     AYRRPYFRQHFCATFVRAGRFLLDGAPQPHILDLGCGCGTQSLLLALAGARVTALDRD
                     PLALRILEKRKRYYEELVGRSLDIRLHAADALTFPYVDLAPLTGVYSMFALNMMQPTS
                     SLMSALTPGLAPGARLAVLDGNNQAWLPRLYPPRRRRVWSPTEFRRELEAIGFRIHEH
                     VGGVALPPFFWVSGDWRPVRALDAQLCKNWLFPISHQILAELQRPVPNQAE"
     gene            859..2052
                     /locus_tag="PLQ54_06460"
     CDS             859..2052
                     /locus_tag="PLQ54_06460"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOS92937.1"
                     /translation="MAILDRVFARLSRVTSTGRFVPQIDGLRFVAIAVVILFHTARVL
                     EEFVPTRVAQWPEDPLWILYKQGFCGVQLFFGISGFILALPFAEQHIAGQRPVSLARY
                     FLRRVTRLEPPYFINLFSFFILRTVLIGESAQQLLPHLLASIFYVHNIAYDTSSTMVN
                     PVAWSLEIEVQFYIMAPLISRLFTIRNTSRRRAAIVALALAFGALRAALVRPESEHGW
                     NLITELPFFLVGFLLADLYLVDWHKQPKQTFLWDVFSGLAWAALFPWIAGTYLDGLVT
                     PILIFIAYVGAFRGVLGSRVFSNPWLVTIGGMCYTIYLYHYAILMTLGPRTVHLWAGP
                     GRWADALFQTALLFPPVILVSAGLFAAFEKPFMQRDWPSVWRTTIRGWLRRPSVEASP
                     GGGEG"
     gene            2073..>3669
                     /locus_tag="PLQ54_06465"
     CDS             2073..>3669
                     /locus_tag="PLQ54_06465"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOS92938.1"
                     /translation="MAGKLARGIGIARHMGIGWCAYRGVYELARRSGWLRLRSPARPW
                     EAFSLDRVLSDPSLAEPESLLDRRRQGSRFLFDSARLTGAKGRFEAWDAGEETPQSIQ
                     LAESVLAGRIRFFEHSVHALGFPPDWHRDPYTGARVSAEAHWSQVPLLGASDIKAVWE
                     ANRFGWAYALARAYARTHDDRYAEAFWVALEDWCDRNPPGLGPHYACGQEVSLRVMAW
                     AFALHAFMDSPATTADRVARMLQALGASGQRIEANIGYALSQRNNHGLSEGAGLFTLG
                     AVFPEFRAARRWLTRGHRVIERLSRELIDEDGAFSQHSANYHRVALQVLSWTVALGDR
                     AGHPLSDVARERLGRLASLLRAIQDETSGRVPCFGQNDGALVLPLSNCGYEDFRPALA
                     GAWHVATGGRLYEDGPWDEECWWLGGATEPGAGCDGPTARAVGDTSALVSGYFAIRSN
                     EGMAFTRCGRFRHRPAQADVLHVDIWWRGTNVACDAGTYSYNQPPPWDGGLGGTSYHN
                     AVTVDGCDQMERAERFLWLPWVRG"
BASE COUNT          543 a         1217 c         1209 g          700 t
ORIGIN      
        1 ccttagggca ctgggacccc caagccacgt ggcatttcta cctggggatc tccgatgaga
       61 tcgagtccgg ccacctcgat tcgggcgagc gtgagcacat ggcccgctac tatgccgagg
      121 ctggccttct gcgggcttac cgccgtccgt actttcgtca gcacttctgc gccacgttcg
      181 tgcgtgccgg ccggttcctt ctggacggag cgccacagcc gcacattctg gacttgggct
      241 gcggctgcgg gacccagagc ctcctgctgg ccttggccgg ggctcgggtg accgcgctgg
      301 accgggatcc cctggccctt cgcatcctcg agaagcgcaa gaggtactac gaggagctgg
      361 tgggccgctc cctcgatatc cgtctgcacg ccgccgatgc gctcacgttc ccctacgtcg
      421 atctagcgcc gctgaccgga gtctactcca tgttcgcgct gaacatgatg cagccgacgt
      481 cgagtctgat gtcggccctg accccgggct tggcccccgg tgcacggctg gcggtcctcg
      541 acggaaacaa ccaggcatgg ctcccgagac tctacccgcc acgccggcgc cgtgtctggt
      601 cccccaccga gtttcgccgg gagctggagg ctatcggatt ccggatccat gaacatgtcg
      661 gtggagtggc cctgccgccg ttcttctggg tgtccggcga ctggcggcca gtccgggcac
      721 tggacgcgca actctgcaag aactggttgt tccccatctc ccaccagatt ctggccgaac
      781 tgcaaaggcc cgtgcctaac caggccgaat aggcccgcgg cccagaggga gaggtggacg
      841 gcagccggcg ttccgtctat ggctatactc gaccgcgtct tcgctagact atcgcgcgtc
      901 acctcgacgg gccgcttcgt gccccagatc gatggcctgc ggtttgtcgc catcgccgtg
      961 gtcatcctgt tccacacggc gcgggtgctt gaagagttcg tgcccacccg ggtggcgcaa
     1021 tggcctgagg accccctctg gatcctctac aagcagggat tctgcggagt ccagctcttc
     1081 ttcggcatca gtgggttcat tctggcgctg cccttcgcgg aacagcacat cgcaggccag
     1141 cgacccgtct cgctcgcacg atacttcctg cggcgcgtca ctcggctgga gccgccctac
     1201 ttcatcaacc tgttctcctt cttcatcctg cgaacggtgc tgattgggga gtcggcacag
     1261 cagcttctac cacacctgtt ggccagcatc ttctacgtcc acaacatcgc ctacgacaca
     1321 tcgagcacca tggtgaatcc ggtggcatgg tctctcgaga tcgaggtcca gttctacatc
     1381 atggctcccc tgatctctcg cctgttcacc atccggaaca cgtcacggcg ccgggccgcc
     1441 attgttgcac tggctctggc gttcggtgct ctgcgcgccg cattggtgcg gccggagagc
     1501 gagcatggat ggaacctgat caccgagctg ccattcttcc tcgttgggtt cctgctcgcc
     1561 gatctttacc tggtggactg gcacaagcag ccgaagcaga cgttcctctg ggatgtgttc
     1621 tccggcctcg cgtgggcggc tctgttccct tggatcgcgg gcacatatct tgacggtctg
     1681 gtgacaccga tcctgatctt catcgcctat gtgggtgcat tccgcggggt actcggaagc
     1741 cgggttttct ccaatccttg gctggtgacc atcggcggca tgtgctacac gatctacctc
     1801 taccactatg cgatcctgat gacactcggc ccaaggaccg tgcatttgtg ggccggtccg
     1861 ggccgttggg cggatgctct attccagacg gccctgctgt tcccgccggt catcctcgtg
     1921 tcagcgggtc tcttcgcggc gttcgagaag ccgttcatgc agcgcgactg gccgtcggtg
     1981 tggcgcacaa cgattcgcgg ctggctgaga cggccttcgg tcgaggcttc cccgggagga
     2041 ggcgaagggt aggctcggct gacccgtggc tcatggcagg taagctcgcg cgtggcatcg
     2101 ggatcgcccg gcatatgggg atcgggtggt gtgcctaccg gggcgtgtat gaactggcgc
     2161 gtcgatccgg ctggctccgc ctccggtcgc cggctcgccc gtgggaggcg ttctcgctcg
     2221 atcgggtgct gtcagacccg tcgctggccg aacccgagtc actgctggac cgtcgacgcc
     2281 aggggtcccg cttcctgttc gactccgcgc gactcaccgg ggcgaagggt cgtttcgagg
     2341 cctgggacgc gggagaggag acacctcagt cgatccagct ggcggagtcc gtgctggccg
     2401 gccgcatccg gttcttcgag cactcggtgc acgcactggg tttcccccct gactggcaca
     2461 gggaccccta cacgggtgcg cgcgtctccg ccgaggccca ctggtcccaa gtgcccctgc
     2521 tgggtgcctc cgatatcaag gccgtatggg aggcgaaccg tttcggctgg gcgtacgctc
     2581 tggcccgggc ctacgcgcgc acgcacgatg accggtatgc cgaggcattc tgggtggcac
     2641 tcgaggactg gtgcgaccgg aacccgcccg gactcggacc gcactatgcc tgcggccagg
     2701 aagtcagcct gcgcgtgatg gcgtgggcct tcgccctgca cgctttcatg gacagcccag
     2761 cgaccacggc tgaccgggtg gcacggatgc tgcaggcact cggcgcgtcc ggtcagcgca
     2821 tcgaggccaa catcggctat gcgctgagcc agaggaacaa ccacggactc agtgagggcg
     2881 cgggcctctt caccctcggt gccgtgttcc ctgagttccg cgcggcgcgg cgatggctca
     2941 cccgaggcca ccgggtgatc gagcgcctgt cacgggagct gatcgatgag gacggggcgt
     3001 tcagccagca ttcggccaac taccaccggg tggcgctgca ggtgctctcg tggacggtgg
     3061 ccctgggcga ccgtgccggc catccgctat ccgatgtcgc gcgggagcgc ctgggccgtc
     3121 tggcctctct cctgcgagcg atacaggacg agaccagtgg ccgcgtgccg tgctttggcc
     3181 agaatgacgg cgcactggtg ctgccgctga gtaactgcgg gtacgaggac ttccggccgg
     3241 cgctggcggg agcatggcat gtggccacgg gcggccgtct gtacgaggac gggccatggg
     3301 acgaggagtg ctggtggctc ggcggcgcca ccgagccagg cgcgggatgc gacggcccca
     3361 cggcgcgggc cgtcggagac acgagcgcgc tcgtgagtgg ctacttcgcc atccgaagca
     3421 atgagggaat ggccttcacc cgctgcgggc gattccgcca ccgccccgcc caggccgacg
     3481 tgctgcatgt cgatatctgg tggcgcggga ccaacgtcgc atgtgacgcg ggcacctaca
     3541 gctacaacca gccaccaccg tgggatgggg gactgggagg gacatcgtac cacaatgccg
     3601 tcacggtcga tggctgcgac cagatggagc gcgccgaacg gttcctctgg ctgccctggg
     3661 tgcggggct
//
LOCUS       DAOSPL010000280         3665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25316_length_3665_cov_5.125485, whole genome shotgun sequence.
ACCESSION   DAOSPL010000280 DAOSPL010000000
VERSION     DAOSPL010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25316_length_3665_cov_5.125485"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..612
                     /locus_tag="PLQ54_06470"
     CDS             193..612
                     /locus_tag="PLQ54_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOS92939.1"
                     /translation="MAVCTRMISVRSRGDCDVTDITPQVAEAIEDSGLLEGLVHVFVT
                     GSTAGVTTIEFESGLLSDVKGFFERIAPQGDVYAHNERWHDGNGHSHVRASLLGPSIC
                     VPVIGGKMTLGAWQQIVLVDFDNRPRNRELVVQVIGE"
     gene            646..1545
                     /locus_tag="PLQ54_06475"
     CDS             646..1545
                     /locus_tag="PLQ54_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOS92940.1"
                     /translation="MQLRIAQAQISPVFGDYAANLARFGELAAQAVDEGCDLLTLPET
                     ALSGYFLEGAVIEVARPATTVLADLCASYRAACPAGRPLDVLVGFYELYESNVYNSAL
                     YATLSAADEPALLHVHRKVFLPTYGVFDEQRFVARGHRIGAFDTRFGRTAILICEDVW
                     HSMTAAIAALRGARFIHTVSSSPARGFAGEVPWNVGKWLELLQSIAQEHAVFVTHTAR
                     VGFENGKGFAGGSAAVAPDGRIMARGPSLGEALLCATLDLDDLSVARANQPLGTDLAE
                     AAGDLATEFADAVREARGEGTWA"
     gene            1536..2426
                     /locus_tag="PLQ54_06480"
     CDS             1536..2426
                     /locus_tag="PLQ54_06480"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013456985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOS92941.1"
                     /translation="MGVRLPVVRGTQPDPADHSVLAVNAALLTDWLVAFLRDEVTVRR
                     PFRTVVVGVSGGVDSAVTAHLCARALDPANVLGVRMPYRTSSPDSLEHAQLVIEGLGI
                     RSVTVDISDAVDGLLRSEPDASPLRRGNVMARMRMIVLFDQAAKHHAIPVGTGNKTER
                     MLGYYTWHGDDAPPVNPLGDLLKTQVRALARHLGVPEAVVAKPPSADLVRGQTDEADI
                     GVAYENADMILHYLLQGYSAARLFEWGFCPADVHVVERRVNQTRWKRHLPATAMISST
                     AIGESHLRPTDYAPPAALPG"
     gene            2602..3441
                     /locus_tag="PLQ54_06485"
     CDS             2602..3441
                     /locus_tag="PLQ54_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015847111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS92942.1"
                     /translation="MKKSINIWSFPGDLPLAEKMRMAKDAGFAGIELEMADEGPVSLT
                     STEADLRAVADMAAEAGLAVSSLATGLFWGNCGTSDNPATRAKAREIAEKMIAAAPLL
                     GTDAILLIPGAVDVFFMPDSEVIPYDVAYDRALELVKSLAPSAEKHQVHIGVENVWNK
                     LFMSPLELAGFIDAAGSPWIGSYFDVGNVVKFGYPEQWIRILGKRIKRVHFKDYRIDA
                     GFPQGFVDLLAGDVNWPEVMKAFREVGYDGWVAPEMIPPYKHCTQQIIYNSSAAVDAI
                     LGL"
BASE COUNT          534 a         1262 c         1280 g          589 t
ORIGIN      
        1 ccatgacgac ctcctggccc tgctccacgg cgagcatctg gcgccgcccg agccggatgg
       61 cgggatgtga gttcgcgccc gcgccgcgcc atcggcacgc gggcgaccga tggggtatgc
      121 tatcggggcc gatcgttgcc ggcggcctgg ggcccatgag gtgcccgctg ccgcacggag
      181 ggagaggaca ccatggctgt ctgcacccgg atgatctcgg tgcgttcgcg gggcgactgc
      241 gacgtcacgg acatcacacc gcaggtcgcc gaggccatcg aggactcggg actgctggag
      301 ggtctcgtcc acgtcttcgt caccggatcg accgccggcg tcacgaccat cgagttcgag
      361 tccggcctgc tgtccgatgt gaagggattc ttcgaacgca ttgcgcccca gggcgacgtg
      421 tacgcccaca atgagcgctg gcacgacggc aacggccatt cccacgtgcg cgcctccctg
      481 ctcgggccat ccatctgcgt gccggtcatc ggcgggaaga tgacgctggg cgcatggcag
      541 cagatcgtgt tggtggactt cgacaaccgg ccccgcaacc gggagctcgt ggtgcaggtc
      601 atcggcgagt gatcgcggcc ggtgggtggc tccggaggta ctggagtgca gctacggatc
      661 gctcaggcgc agatctcccc ggtcttcggc gactatgccg ccaacttggc ccgattcggc
      721 gaactcgccg cccaggctgt ggacgagggc tgtgacctgc tgactctgcc cgagaccgcc
      781 ctctcgggct acttcctcga aggtgccgtc atcgaggtgg cgcgccccgc gaccacggtg
      841 ctggccgacc tctgcgcatc gtaccgcgcg gcctgcccgg cgggccggcc cctggacgtt
      901 ctcgtcgggt tctacgagct ctacgagagt aacgtgtaca actccgcact gtacgcgacg
      961 ctgagtgccg cggatgagcc cgcgctgctg catgtgcacc gcaaggtgtt tctgccgacc
     1021 tacggcgtgt tcgacgagca gcgtttcgtg gcgcgcgggc atcggatcgg tgccttcgac
     1081 acgcggttcg gacggacggc catcctgatc tgcgaggacg tttggcactc catgaccgcc
     1141 gccatcgcgg cgcttcgggg cgcccggttc atccacacgg tgtcgtcgag ccccgcccgg
     1201 ggcttcgcgg gcgaggtgcc ctggaatgtg ggcaagtggc tcgagctgct gcagagcatc
     1261 gcccaggagc atgccgtgtt cgtgacgcac acggcccgtg tgggtttcga gaacggcaag
     1321 ggtttcgccg gcgggtcggc cgcggtggcg cccgacggcc ggatcatggc ccgaggcccc
     1381 tcactcggcg aggcgctgct gtgtgccacg ctcgatctcg atgacctgag tgtggcccgc
     1441 gcgaaccagc ctctgggcac cgacctggcc gaggcggcgg gcgacctggc caccgagttc
     1501 gccgatgcgg tccgcgaggc ccgcggagag gggacgtggg cgtgagactg cccgtggtgc
     1561 gcggaacaca gcccgatccg gccgaccact cggtgttggc cgtcaatgcc gcgctgctca
     1621 ccgactggct cgtggccttc ctccgggacg aggtcaccgt ccgccgcccg ttccggaccg
     1681 tggtcgtggg cgtctccggc ggcgtcgact cggccgtcac cgcccatctg tgcgcccgcg
     1741 ctctggaccc cgccaacgtc ctcggtgtgc ggatgcccta tcggaccagc agccccgata
     1801 gcctggagca cgcgcaactg gtcatcgagg gcctgggcat ccggtcggtg accgtggaca
     1861 tctccgatgc ggtggacggc ctgctccgct ccgagcccga tgcctccccc ctgcgccggg
     1921 gcaatgtcat ggcgcgcatg aggatgatcg tgctcttcga ccaggccgcc aagcaccacg
     1981 ccatcccggt gggcaccggc aacaagacgg agcgcatgct cgggtactac acctggcacg
     2041 gcgacgacgc gcctccggtg aatccgctgg gagacttgct caagacccag gtgcgggccc
     2101 tcgcccggca tcttggggtg cccgaggccg ttgtggccaa gcccccatcg gccgacctgg
     2161 tccgcggtca gaccgacgag gccgacatcg gggtggccta tgagaacgcc gacatgatcc
     2221 tgcactacct gctgcagggc tactcggccg cccggttgtt cgagtggggt ttctgccccg
     2281 ccgatgtgca cgtggtcgag cgtcgcgtca accagacccg ctggaagcgt cacctgccgg
     2341 ctacggccat gatctcatcg acagccatcg gcgagtcgca cctgcggccc acggactacg
     2401 ccccgccggc ggccctgccc ggatagcgcg ggcggtcgtc tgccggccgg cggcgtcggg
     2461 gcgagccggc ccacccgtgc gggcagcagg gccatcggcc gtcgcgcgcg aactgcatcc
     2521 ctcgccagcc cgtgagggct gccgcgtata cgtcgggcgg tccggaagct cgctgggccg
     2581 ctcccggagg cgagacggcg catgaagaag agcatcaaca tctggtcctt ccccggcgac
     2641 ctgcccctgg cggagaagat gcgcatggcc aaggacgccg gcttcgcagg tatcgagctc
     2701 gagatggccg acgagggccc cgtgagcctg acatcgaccg aggccgacct ccgagcggtc
     2761 gccgacatgg cggccgaggc cgggctggcc gtctcctcac tggccaccgg cctgttctgg
     2821 ggcaactgcg gcaccagcga caaccccgcc acgcgggcca aggcccggga gatcgcggag
     2881 aagatgatcg ccgccgcgcc gctgctcggc accgatgcca tcctgctgat ccccggcgcc
     2941 gtcgatgtct tcttcatgcc cgactccgag gtcatcccct acgatgtggc ctacgatcgc
     3001 gccctcgagt tggtgaagtc cctggcgccc tcggccgaga agcaccaggt gcacatcggt
     3061 gtcgagaatg tctggaacaa gctgttcatg agccccctgg agctggccgg gttcatcgat
     3121 gcggcgggca gcccgtggat cgggtcgtac ttcgatgtgg gcaatgtggt gaagttcggc
     3181 tatcccgagc agtggatccg catcctcggc aagcgcatca agcgggtgca cttcaaggac
     3241 tatcggatcg acgccgggtt cccgcagggg tttgtggacc tgctggcggg cgacgtgaac
     3301 tggcccgagg tcatgaaggc gttccgggag gtgggttacg acggctgggt ggcgccggag
     3361 atgatcccgc cgtacaagca ctgcacccag cagatcatct acaacagctc ggctgcggtg
     3421 gacgcgatcc tggggctgta ggatcagggg cggtccgaat gcctttcgga ggggccgaat
     3481 aggggcattg gcggtcactt gggggcgccc gtggcccatc agcctggttg cggaggggcg
     3541 ccatgggggg aaccacgcgt ctcccagcgt ggtgggcccc caacgcgccc gatccgcacg
     3601 cctcaacctc gggccggtca ggggccccgc gccctccggg cgcacccctg tgcggcccct
     3661 ccacg
//
LOCUS       DAOSPL010000281         3665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25321_length_3665_cov_2.768421, whole genome shotgun sequence.
ACCESSION   DAOSPL010000281 DAOSPL010000000
VERSION     DAOSPL010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25321_length_3665_cov_2.768421"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="PLQ54_06490"
     CDS             complement(<1..1308)
                     /locus_tag="PLQ54_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOS92943.1"
                     /translation="MVPTRTYIKTERYPHRMIPAPQTLADTLVTQLIDAGIDTVFGIP
                     GLYNMPLFDALRRAEGIRVVTVRHEQGAAFMADGYARATGRPAAICLLPGCGVLNAMT
                     GISEAFFDSSPMLVLASQVATEHIDQDRGLLHELTGQLDVVGSVTKHSVRLTDVESAP
                     AVFADALRRLRTGRPRPVQVEIPLDLQSADVSGRPEPTGPADATGRLDHDLIRRAAAN
                     LAAASRPLIYVGGGAVDAASITDIAEHLGAPTFTTGMGVGALPGDHPLWAGVPWVASG
                     DIRPLFEASDCVLFVGTRLNHGMTAEWGIGAPAGSVRIDVDPVEITKTLDVDVQIVAD
                     AAAATEALLTELRRLGPAKGPSAEMAALLEQRRAALAGRVGTTTPWFEALRSALPADG
                     IIAADMSLFWGDMIGSFPVAGPRQILFPWGMGTLGFGVPSALGA"
     gene            1292..2047
                     /locus_tag="PLQ54_06495"
     CDS             1292..2047
                     /locus_tag="PLQ54_06495"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOS92944.1"
                     /translation="MRVGTITSGIRCVSGKVAGHCRPPWALCCDLTKGTTVSAAAWDA
                     GTPLTGTPGQRIRTRRLALGLRLADLAEQAGVSVSFLSQVERDRASASVQRLHMISEA
                     MGLPMAALFGETPAEAHGTDSEDVHEHGQPRRTLASVVRADRRLTVTYPGSLIRNELL
                     VPDLRRALSMMYVVIPVGADTGSEPLVHEGEECGFVVSGQVGVWVGEGDDLQYFELGP
                     GDAISQSSEIPHRSKNIGDVPAVIVTAQTPPSL"
     gene            2243..>3665
                     /locus_tag="PLQ54_06500"
     CDS             2243..>3665
                     /locus_tag="PLQ54_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020551932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOS92945.1"
                     /translation="METYDVVIAGGGHNGLMCGAVLAKEGLKVLVLERHVRLGGGCTT
                     AEVTLPGFKHDLYGSSHVWIHCNPEFRAMLPELERFGLKYIWANNHITGHPDKVGKGI
                     VVYRDVDRTCASIAQYSEKDAKRYREIYSGFAEIEDGFLTGMFSPPNPPSLMQSMLES
                     TDDGQEWLRNYAMSPHDFTMENFEHPVVRTMIMGWATAPGIRPDQEGRGEMFYIMIPA
                     IHSYGESIPEGGSIELPNALARYIEHYGGTVLLEAEVSKFTVDEKGVATGVELSDGRS
                     FRARKAVVTGLNPKLTYAMFDDGVLTPSFQKKVDGYLPGDFTIVRAHFAIKEPPKYTC
                     GDEVNHTPFQRIYGSVESITKQWADISVGELPLDPFLWVACWTTKDPTRAPAGKHTLI
                     MDTFVPIDLADGRRWDAVADDYIWKVQLPKLREYAPNMTESNILGSFIQTGPSIEADN
                     PCFVNGSTTGGAMRMFQSGYFRPF"
BASE COUNT          645 a         1224 c         1224 g          572 t
ORIGIN      
        1 cgcgccgagc gcgctgggga cgccgaagcc gagcgtcccc atgccccacg ggaagaggat
       61 ctggcgcggt cccgccaccg ggaaactacc gatcatgtcg ccccagaaca ggctcatgtc
      121 ggcggcgatg atgccgtcgg cgggcagtgc gctgcggagt gcttcgaacc agggcgtcgt
      181 cgttccgacc cgtccggcga gcgcggcgcg acgctgctcg agcagggccg ccatctcagc
      241 cgacgggccc ttcgccggcc ccagcctgcg cagttcggtg agcaacgcct cggtcgcagc
      301 ggccgcgtcg gccacgatct ggacgtcgac gtcgagggtc ttggtgatct cgacggggtc
      361 gacatcgatg cggacggacc cggccggtgc cccgatgccc cactcggcgg tcatgccgtg
      421 gttgaggcgg gtgccgacga acagcacgca gtcgctggcc tcgaacagcg gccgaatgtc
      481 gcccgacgcc acccacggaa cccccgccca caaggggtga tcgccaggca aagcgcccac
      541 gcccatgccc gtcgtgaacg tcggcgcgcc caggtgctct gcgatgtcgg tgatggacgc
      601 cgcgtccacc gcgccgccgc ccacgtaaat caggggcctg gacgccgccg ccagattcgc
      661 ggcggccctg cggatgaggt cgtggtcgag tcgtccggtg gcgtcggcgg gcccggtcgg
      721 ctcggggcga ccggagacgt cagcgctctg caggtcgagg gggatctcaa cttggacggg
      781 gcgcgggcgt ccggtgcgga gccggcgaag ggcgtccgcg aaaacggcgg gagccgactc
      841 gacgtcggtg aggcgcaccg aatgcttggt gacagaaccg acgacgtcga gctggccggt
      901 gagttcgtgg aggagcccgc gatcctgatc gatgtgctcg gtcgcaacct ggctggccag
      961 cacgagcatg ggcgaggagt cgaagaacgc ctcggagatg cccgtcatcg cgttcagcac
     1021 cccgcagccg ggcagcaggc agatcgcagc ggggcgaccg gtcgctcggg cgtaaccgtc
     1081 agccatgaac gctgcaccct gctcgtgacg caccgtcacc actcggatgc cctcggcccg
     1141 acgtagcgcg tcgaacagcg gcatgttgta gaggccggga atgccgaaga cggtgtcgat
     1201 gccggcgtcg atcaactgcg tgaccagggt gtctgccaga gtctggggag cggggatcat
     1261 tcgatggggg tacctttccg ttttgatata ggtgcgtgtc ggcaccataa catccgggat
     1321 cagatgtgtc agcggcaaag tggccgggca ctgcagaccc ccttgggcgc tatgttgtga
     1381 cctgacgaaa ggcaccacag tgagtgcagc agcgtgggac gcaggcaccc ccctcaccgg
     1441 cacgccaggg caacgcatcc ggacgcgccg gctcgcccta ggcctgcgtc tggccgacct
     1501 cgccgagcag gccggcgtgt cggtgtcgtt cctcagccag gtggagcgcg accgcgcgtc
     1561 ggcgtccgtt caacgactgc acatgatcag cgaggcgatg gggcttccga tggcggctct
     1621 cttcggcgag acgccggccg aggcacacgg cacggacagt gaggacgtgc acgagcacgg
     1681 gcagccgcga cgcacgctgg cgtccgttgt gcgggccgat cggaggctca ccgtgacgta
     1741 tccggggtcg ctgatccgca acgagctgct cgtgcccgac ctgcgccgag cgctctcgat
     1801 gatgtacgtg gtgatccccg tcggggccga caccggcagc gaaccgctgg tgcacgaggg
     1861 tgaggagtgc ggcttcgtcg tctccggcca ggtgggcgtg tgggtcggcg agggcgacga
     1921 tctgcagtac ttcgagctgg gcccgggtga tgcgatcagc cagtcgagcg agatcccgca
     1981 ccgcagcaaa aacatcggcg acgttcccgc ggtgatcgtc accgcccaga cgccgccgtc
     2041 gctctgaacc tccaaacgtc gtaagtcgcc gcgaacccac cggccaaacg cgtgtgggga
     2101 ctccacctga ccccgtctcg gacgcgggtg gatggcctgc tgacgtaaag gatttcccta
     2161 tcctcgcagc gttgactcac catgccgcct cgaccatgct tcatttcgat atcactgaag
     2221 tccggtgaag tgaggtttca acgtggagac atacgatgtc gtcattgctg gcggtggaca
     2281 caacggtctg atgtgcgggg ccgtgctcgc caaggaggga ctcaaggtgc tcgtgctcga
     2341 gcgccatgtg cgcctcggcg ggggctgcac gaccgcggag gtgacgctgc cgggcttcaa
     2401 gcacgacctg tacggcagct cccacgtctg gatccactgc aacccagagt tccgggccat
     2461 gctgcccgaa ctcgaacggt tcggcctcaa gtacatctgg gccaacaacc acatcaccgg
     2521 acaccccgac aaggtcggca agggcatcgt cgtgtaccgc gacgtcgacc ggacctgcgc
     2581 ctcgatcgcg cagtactccg agaaggacgc caagcggtac agggagatct actccggctt
     2641 cgccgagatc gaggacggct tcctcaccgg catgttcagc ccaccgaacc cgccgagcct
     2701 catgcagtcg atgctggaga gcaccgacga cggccaggaa tggctccgca actacgcgat
     2761 gagcccccac gacttcacga tggaaaactt cgagcacccc gtcgtccgga cgatgatcat
     2821 ggggtgggcg accgcgcccg ggatccgtcc tgatcaagag ggtcgcggcg agatgttcta
     2881 catcatgatc cctgcgatcc actcgtacgg ggagtcgatc cccgagggcg gcagcatcga
     2941 actgccgaac gccctggccc gctacatcga gcactacggc gggacggtcc tgctcgaggc
     3001 cgaggtgtcg aagttcaccg tggacgagaa gggcgttgcc accggtgtcg agctgtccga
     3061 cggacgctcc ttccgggccc gtaaggctgt cgtcaccggc ctcaacccca agctcaccta
     3121 cgcgatgttc gacgacggcg tcctgacccc ctcattccag aagaaggtcg acggctacct
     3181 tccgggcgac ttcacgatcg tccgggcgca tttcgcgatc aaagagcccc cgaagtacac
     3241 gtgcggtgac gaggtcaacc acaccccgtt ccagcgcatc tacggctcgg tcgagtcgat
     3301 caccaaacag tgggccgaca tctccgtcgg cgagttgccg ctcgacccat tcctgtgggt
     3361 cgcctgctgg accaccaagg atccgacgcg ggcaccagcc ggcaagcaca ccctgatcat
     3421 ggacaccttc gtgcccatcg acctggccga cgggcgccgc tgggacgccg tcgccgacga
     3481 ctacatctgg aaggtgcagc tgccgaagct gcgcgagtat gcgcccaaca tgaccgagtc
     3541 gaacattctt ggctcgttca tccaaacggg tccgagcatc gaggccgaca acccctgctt
     3601 cgtcaacggc agcaccaccg gtggagcgat gcgtatgttc cagtcgggct acttccgtcc
     3661 gttcc
//
LOCUS       DAOSPL010000282         3661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25350_length_3661_cov_3.356073, whole genome shotgun sequence.
ACCESSION   DAOSPL010000282 DAOSPL010000000
VERSION     DAOSPL010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25350_length_3661_cov_3.356073"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLQ54_06505"
     CDS             complement(<1..280)
                     /locus_tag="PLQ54_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOS92946.1"
                     /translation="MELQMSTRERDRLKVMHQVEQGQLSRGVAARQLGVSERQLRRLL
                     RRYREEGDAGLIHRLRGRASNRRTGEEVRERAVEVLGGELRGFGPTLAS"
     gene            453..680
                     /locus_tag="PLQ54_06510"
     CDS             453..680
                     /locus_tag="PLQ54_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018140642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOS92947.1"
                     /translation="MQLVQGTVKWFSNDKGYGFITPQDGSEDVFVHFSAIQGSGFRSL
                     DEGEEVEFDVEQGAKGPQAANVKRQGRSASF"
     gene            complement(775..1692)
                     /locus_tag="PLQ54_06515"
     CDS             complement(775..1692)
                     /locus_tag="PLQ54_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOS92948.1"
                     /translation="MQLGFASAILPDLDRNEVLAFAREGNFTCVELMCWPKGAAERRY
                     AGVTHIEVETVDDEAAKRIRDEAASAGVRISGLGYYPNALSPDPAEAEAAVAHIQKVI
                     EASARLGVGLVNTFVGRDWTKSVDENWPRFLDTWRPLIAFAEERGVRVAIENCPMLFS
                     RDEWPGGKNLAHSPAVWRRMFEDIPSPSFGLNFDPSHLVWQGLDIPSAIREFGSRIFH
                     THAKDARIDRHKLADVGILAFPLQFHTPKLPGLGDVDWGAFFSALTDTGYDGPVCIEV
                     EDRAYEGSLETRKASLRQSADFLRQFVPD"
     gene            complement(1704..2738)
                     /locus_tag="PLQ54_06520"
     CDS             complement(1704..2738)
                     /locus_tag="PLQ54_06520"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS92949.1"
                     /translation="MDSAGVGIIGLGMGRSQLQAFRDNPLTRVVGICDTDEARLRDVA
                     EQVHPDVATVDYRDLMARDDISIICVASPDPRHREHSVAALEAGKHVFCEKPLALDYA
                     DCEAIVRAADRAPGVFMIGQVCRFAPGFVAAKRLIEQGTIGDLFLVESEYAHDYTHVG
                     GTGGWRKDPKRHPVIGGGCHAVDLLRWMAGNAHEVCAFANHKVLTDWPVDDCTVAILR
                     FGNDIIGRAMVSIGCKRDYTMRSCFYGTKGTIICDNTSTHITLHTQDTKRIGLPALGD
                     PQSVGVRIPVGSQAKPVAAEVDHFVHLVTTGAANEMDAREGARTVATCLAIVESSRAG
                     GRPAAVRNEF"
     gene            2995..>3661
                     /locus_tag="PLQ54_06525"
     CDS             2995..>3661
                     /locus_tag="PLQ54_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS92950.1"
                     /translation="METRLSRRGFLRRAATASAGMAIPWIVSGRALGADGKVPASERV
                     TLGFIGTGGMNNAHLGGILGNPRCTVLAVCDVDQGRRLAAQQRVGGQCAAYNDYRELL
                     DRQDIDAVFVATPDHWHTLISIDACKAGKDIYCEKPLTLTIGEGKALVDAVRRYGRVL
                     QTGSQQRSSWEFRQACELVRNGRIGRVHTVWTNIGSAPGGEWAPDEPVPAGLDWDMWL
                     GPAP"
BASE COUNT          600 a         1188 c         1224 g          649 t
ORIGIN      
        1 cggatgccag cgtcggacca aaaccccgca gctcaccccc cagcacctcc accgcccgct
       61 cgcgcacctc ctctccggtc cggcgattcg atgcccgacc ccgcaaccgg tggatcagcc
      121 ccgcgtcccc ctcctcacgg taacgccgca acaggcgcct caactggcgc tcgctcacac
      181 ccaactgcct cgccgctact ccccggctca actggccctg ctccacctgg tgcatcacct
      241 tcagccgatc ccgctcccga gtgctcatct gcagctccat gtcctgtcat cgccctacag
      301 gacattccta ctttgcacac cccggacagc actacgttgc gccgacacga caagaaaaac
      361 attgacgggc tggtacgctc gtgttataag tgctttcctg agggtaaagc acatgcgtag
      421 ctcgtggccc tcagaaaatc tacgtgtagg aagtgcaatt agtgcaggga acagtcaagt
      481 ggttcagcaa cgacaagggt tatggcttca taaccccgca ggacggcagc gaggacgtgt
      541 tcgtccactt ctcggccatc cagggctccg gcttccggtc tctggacgag ggtgaagagg
      601 ttgagttcga cgttgagcag ggcgcgaagg gcccgcaagc ggcgaacgtg aagcgccagg
      661 gccgttcggc ctccttctaa ccagcgcccg attacatatc gcgcacaaca ggcagacatg
      721 ctgagacagc tccggacatc cgcccggagc tgttctccgt acgggcttta tggcctagtc
      781 tggcacgaac tgccgcagga agtccgcgct ttgcctgagg gaggccttcc gcgtctcgag
      841 ggagccctca taggcccggt cctcgacctc gatgcagacg ggtccgtcgt agccggtgtc
      901 ggtcagtgcg gagaagaagg ctccccagtc cacatcgccg aggccgggta gcttcggtgt
      961 gtggaactgc agggggaaag ccaggatacc gacgtcggcg agcttgtggc ggtcgatgcg
     1021 cgcatctttg gcgtgcgtgt ggaagatgcg cgagccgaat tcgcgaattg ccgacggtat
     1081 gtcgagccct tgccagacca ggtgagacgg gtcgaagttg agtccgaagc ttgggctcgg
     1141 gatgtcctcg aacattcggc gccacactgc ggggctgtgg gccaggttct tgccgcccgg
     1201 ccactcgtcg cgcgagaaca gcatggggca gttctcgatg gccacccgga caccgcgttc
     1261 ctcggcgaac gcgatcagtg ggcgccaggt atcgaggaac cgcggccagt tctcgtcgac
     1321 cgacttggtc cagtcgcgcc cgacgaaggt gttgaccagg ccgacgccca gtcgggcgga
     1381 tgcctcgatc accttttgga tgtgcgccac cgccgcctcg gcctcggcgg gatcgggcga
     1441 aagagcgttc gggtagtagc cgaggccgct gatgcggacg ccggctgagg ccgcctcgtc
     1501 acggatgcgc ttcgcggcct cgtcgtcgac tgtctctacc tcgatgtgtg tgactcccgc
     1561 atatcgccgc tccgcggccc ccttgggcca gcacatgagc tcgacgcacg tgaagttgcc
     1621 ctcgcgcgcg aaggccagga cttcgttccg atcgagatct ggcagtatgg cactcgcgaa
     1681 tcccagttgc atgtggggcc tccctagaac tcgttccgca cggcggcagg ccggccgccg
     1741 gcgcggctgg actcgacaat ggccaagcat gtggcgacgg tgcgcgcacc ctcgcgggcg
     1801 tccatctcgt tggcggcacc ggtggtgacc aggtgcacga agtggtcgac ttcggcggcc
     1861 acgggcttgg cttgactccc caccgggatc ctgaccccta ccgactgcgg gtcgccaagg
     1921 gcgggtagtc cgattctctt ggtgtcctga gtgtgtaggg tgatgtgtgt tgacgtgttg
     1981 tcacagatga tggtcccttt ggttccgtag aagcatgagc gcatggtgta gtcgcgtttg
     2041 cacccgatgg acaccatcgc cctgccgatg atgtcgttcc cgaaacgcag tatggcgacg
     2101 gtgcagtcgt ccaccggcca gtcggtgagc accttgtggt tcgcgaaggc gcagacttcg
     2161 tgcgcgttgc cggccatcca gcgcaacaag tcgacggcgt ggcagccgcc gccgatgacg
     2221 gggtggcgct tgggatcctt gcgccaaccg cccgttccac ccacgtgcgt gtagtcgtgg
     2281 gcgtactcgg actcgacgag gaacaggtcg ccaatggttc cctgctcgat gagccgtttg
     2341 gccgcgacga agccgggagc gaaccggcat acttgtccga tcatgaagac tccgggcgct
     2401 cggtcggcgg cccgtacgat ggcctcgcag tcagcgtagt cgagggccag gggtttctcg
     2461 cagaacacgt gcttgccggc ttcgagcgcg gccaccgagt gctcacggtg ccgcgggtcg
     2521 ggcgacgcga cgcagatgat gctgatgtcg tcccgggcca tcaggtcacg atagtccacg
     2581 gtcgccacgt caggatggac ctgctcggcc acgtcccgca ggcgtgcctc gtcggtgtcg
     2641 cagatgccca ccacgcgggt gagcgggttg tcgcggaaag cctggagctg cgaccgcccc
     2701 atgcccaggc cgatgattcc cacgccagct gaatccatcg aggactcctc ctgccccgcg
     2761 cccggagagg catcgggcgc ggtgtctggg ccagttcggt ggcttggccc ggcggtcctg
     2821 cggaatcggc gcctctcgag ggcgaacggc ggcccatcgc gcattgacag cgcggaggtg
     2881 tggctctaag gtgccctccg aggtgccgcg ggaccccgtg ttcctgcgcg gcggcggtcc
     2941 ccgaccgaca cacccgcacc ggcccactgg gttggggcgg agctgaggtg gagcatggag
     3001 accaggctga gcagacgagg tttcctgagg cgggctgcga cggcctcggc gggcatggcg
     3061 atcccttgga tcgtctccgg ccgggctctc ggggccgacg gcaaggtgcc agccagcgag
     3121 agggtcacgc tgggtttcat tgggaccggc gggatgaaca acgcccatct gggcggtatc
     3181 ctcggcaacc cgcgttgcac ggttcttgcc gtctgcgacg tggatcaggg ccgccgcctg
     3241 gcggcccagc agcgcgtcgg aggccagtgc gccgcctaca atgattaccg ggagctgctg
     3301 gaccgccagg acatcgacgc tgtcttcgtc gccacaccgg atcactggca cacgttgatc
     3361 tcgatcgacg cctgcaaggc gggcaaggac atctactgcg agaaaccatt gaccctcacg
     3421 atcggcgagg gcaaggcgct ggttgatgcc gtccggcgct atgggcgtgt gctccagacc
     3481 gggagtcagc agcggtccag ttgggagttc cgccaggcgt gcgagctcgt tcgcaatggc
     3541 cggatcggcc gcgttcacac ggtgtggacg aacatcggta gcgctcccgg tggggagtgg
     3601 gcacctgacg agccggtacc cgcgggtctg gactgggaca tgtggctcgg gccggcgccc
     3661 t
//
LOCUS       DAOSPL010000283         3660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25360_length_3660_cov_2.956449, whole genome shotgun sequence.
ACCESSION   DAOSPL010000283 DAOSPL010000000
VERSION     DAOSPL010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25360_length_3660_cov_2.956449"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="PLQ54_06530"
     CDS             <1..902
                     /locus_tag="PLQ54_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92951.1"
                     /translation="DLRYVARLASQRGGQPIQDVALINPLNQKENAIGWVGTPTNRDA
                     VLNYAVELRRIDNGGQYLAVFHLRAIRYEGVDEPPRAIATVSGPDGRELVQQELQTTG
                     NVQLAAVPVNAPMPGEYRLRLRCGGARYFMGAAASCAYLLPAESPSDSPSEGLARMGI
                     SARTLYQVAEGVTGEVTPASLRELVDPLKQAQNALMWSYRGFDRHRFGVHWRFTADAN
                     IADRKLRMGLQIRVPVGTPQLVLRGPGRAEICRWEVYASPELRTLWFEVRFPSVGEYT
                     LELLDEGVGRADVCLYNALMFDG"
     gene            954..1871
                     /locus_tag="PLQ54_06535"
     CDS             954..1871
                     /locus_tag="PLQ54_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOS92952.1"
                     /translation="MRLLVVGDLNVDLIIAGLPALPALGTEQVGTDCSLSPGGSAANV
                     AAGLAALGAEVSLAARVGEDLFGDYLVQAVAELGVGTAPISRDARHRTGITVALACAS
                     DRAFATYSGAVRELVPEHIADSALATSDHVHVASVYLVPGVGGRIEELLARARHYGCT
                     TSLDTGFDPAEEWEAERIRRALRHCDVFLPNEVELRELTGESDAETAAGGLAALGPTV
                     VAKLGAEGSLACSPDGCVRRAPAKPVRAIDTTGCGDTFNAAFLLRWLERAPLDDCMRF
                     ANAAGALCATRLGGSGVLRGRDEVEAFAG"
     gene            complement(1888..>3660)
                     /locus_tag="PLQ54_06540"
     CDS             complement(1888..>3660)
                     /locus_tag="PLQ54_06540"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92953.1"
                     /translation="ADGTGLRQLTDGPYDDLDPIYLPDGHIMFCTTRGNAYVRCMPPT
                     NAYMLARCDGDGRNIYLVSPNVEPEYLPAVASDGRVMYTRWEYTDKPLWRAQGLWTIN
                     PDGTHTSTLWGNQSVWPDLLKDVRSIPGSSRVMFTGSAHHGWFSGSVGIIDPSKGFNF
                     PNGLTKVTADVEWPESGNGPVDPVESPHYHSSGVYSAYYSPYPLTEKDFLVSANRNGK
                     FVLYLMDVDGNRELIYEGTHHILHAIPLRPRTRPPVLPDSVAWPSREERLNPKPGIIY
                     SSNVYSGAPEALRGKAKFLRVLQVDAKTYTYWNRRPYISTGPVVSILQSEGVKRVLGT
                     VPIRPDGSVSFWAPSGRPLHFQLLDGEQRALQTMRSFVGVMPGEKRGCLGCHELHSTA
                     PAYESLADAVISEPERITPPPWGEDTVSFARYVQPVLDRYCGACHQGEGEARKVLDLT
                     RRPGFLDFDEPYVTLTGHPTWAAPYQRPDPEPPGWGIADTIMTEAYSTVDPKAYVTPE
                     PMQRLSYRSRLIQRASSGEHHGVKVDAESLLRLILWVDTMCPYLGEEEVRAIPDPDFQ
                     GIDWLAIRPRIATAPRVVRPGPVD"
BASE COUNT          578 a         1161 c         1286 g          635 t
ORIGIN      
        1 tcgatctgcg ttacgtggct cgcctcgcga gccagcgcgg cgggcagccc atacaggacg
       61 tcgctctgat caacccgctc aaccagaagg agaacgccat cggctgggtg ggcacgccga
      121 ccaaccggga tgccgtgctg aactacgcgg tggaactgcg ccgcatcgac aatggcgggc
      181 agtacctggc cgtctttcac ctgcgtgcca ttcggtatga gggcgtcgac gagcctcccc
      241 gggccatcgc gacggtcagc gggccggatg ggcgcgagct ggttcagcag gagctgcaga
      301 ccacgggcaa tgtgcagctc gccgcggtgc cggtcaacgc ccccatgccc ggggagtatc
      361 gccttcggtt gcgctgcggt ggcgcgcggt acttcatggg agcggccgct tcgtgcgcct
      421 acctgctgcc tgcagagtcg ccctcggact cgccttcgga gggactggca cggatgggca
      481 tcagtgcccg gacgctctac caggtcgcag agggtgtcac gggtgaggtc acgccggcga
      541 gtctgcgcga gctggtcgac ccactcaaac aggcgcagaa cgcgctcatg tggtcctaca
      601 ggggtttcga ccgtcaccgc ttcggcgtcc actggcggtt cacggccgat gccaacatcg
      661 ctgacaggaa gctacgaatg ggtctgcaga tccgggtgcc ggtgggcacg ccgcaactgg
      721 ttctgcgtgg cccaggcagg gccgagatct gccggtggga ggtctacgca tcgccggagc
      781 tacgcacgct ctggttcgag gtgcgcttcc cctctgtcgg cgagtacacc ctggagcttc
      841 tggatgaggg cgtcgggcgc gccgatgtct gcctgtacaa cgcgctaatg ttcgacggct
      901 agccggcgcg gcgggtgcga gccggggcct ggggtagggg gatggtggcg ccgatgcgcc
      961 tgctggtggt cggcgacctg aacgtcgatc tgatcatcgc cggactgcct gcgctgccgg
     1021 ctcttggtac cgagcaagta ggcaccgatt gcagcctgtc accgggggga tcggcagcca
     1081 acgtggcggc tggactggcg gccctggggg ccgaggtctc attggcggcc cgtgtgggtg
     1141 aggacctgtt cggtgattat ctcgttcagg cggttgcgga gctgggcgtg ggcaccgcgc
     1201 cgatctcgcg ggacgcaagg caccgtaccg gcatcaccgt ggccctggcg tgcgccagcg
     1261 atcgggcctt cgccacgtac tcgggcgccg ttcgggagtt ggtgcccgag cacatcgccg
     1321 actccgctct ggccacgtcc gaccatgtgc acgtggccag tgtgtacctc gttccgggcg
     1381 tgggggggcg catcgaggag ctcctggccc gggcgcgcca ctacggctgc acgacgagcc
     1441 tcgatacggg cttcgaccca gccgaggaat gggaggcgga gcggatccgg cgggcactgc
     1501 gccactgcga cgtgttcctc cccaacgagg tcgagctccg tgagttgacg ggagagtctg
     1561 acgccgagac cgcagcaggc ggccttgccg cgctgggccc cactgtggtg gccaagctgg
     1621 gagccgaggg atccctggcg tgctccccgg atgggtgcgt gcggcgagct ccggcgaagc
     1681 ccgtgcgggc catcgatacg accggctgcg gcgatacctt caatgcggca ttcctgctgc
     1741 gctggctcga acgcgctccg ctggacgact gcatgcgctt cgccaacgcg gcgggggcgc
     1801 tgtgcgccac gcgcctgggc ggaagcggtg tgctgcgcgg gcgtgacgag gtcgaggcat
     1861 ttgctgggtg aggacggccg ttgcgcctca gtcgaccggc cccgggcgaa ccacccgcgg
     1921 cgccgtggcg atgcgcggac ggatcgccaa ccagtcgatg ccctggaagt ccggatcggg
     1981 aattgcccgc acttcctcct cgcccagata gggacacatg gtgtcgaccc acaggatcag
     2041 ccgcagcagg ctctcggcgt cgaccttcac cccgtggtgc tcgccgctgc tggcgcgctg
     2101 gattagacgg ctccggtagg agagcctctg catcggctcg ggtgtcacgt aggccttggg
     2161 atccacggtg ctataggcct cggtcatgat cgtgtcggcg atgccccatc ccgggggctc
     2221 ggggtcgggc ctctggtagg gcgccgccca ggttggatgg ccggtgaggg tgacataggg
     2281 ctcatcgaaa tcgaggaatc cggggcgccg cgtaaggtcc agcaccttgc gggcctcgcc
     2341 ctcgccctgg tggcacgcac cgcagtaacg atcgagcacc ggctgcacgt accgggcgaa
     2401 gctcacggtg tcctcgcccc atggcggcgg cgtgatccgt tcgggctcgc tgatgaccgc
     2461 atcggccaac gactcataag cgggggccgt gctgtgaagt tcatggcagc ccagacagcc
     2521 cctcttctcg ccgggcatga cgccgacgaa gctacgcatg gtctgcagtg cccgttgctc
     2581 accatcgagc aactggaaat gcagcggcct gcccgacggg gcccagaacg atacggagcc
     2641 atccgggcgg atgggcacgg tgccgagcac cctcttgacc ccctcggact ggaggatcga
     2701 cacaaccggt ccggtggaga tgtatggccg ccggttccag taggtgtatg tcttggcgtc
     2761 cacctggagg acccgaagaa acttggcctt gccgcgcagc gcctctggcg ctccgctgta
     2821 cacgttgctg ctgtagatga tgccaggctt ggggttcagc cgctcctccc gtgacggcca
     2881 agccaccgag tcggggagta ctggcggccg ggtccggggc ctcagcggga tggcatgcag
     2941 aatgtggtga gtgccctcgt agatgagctc gcggttgccg tcgacatcca ttagatagag
     3001 cacgaacttg ccgttgcggt tggccgagac cagaaagtcc ttctcggtca gcgggtacgg
     3061 cgagtaatag gcgctgtaga cgccgctcga gtggtagtga ggtgactcga cgggatcgac
     3121 tgggccgttt cccgactcgg gccactcgac gtcggccgtg accttggtca gaccattcgg
     3181 gaagttgaac cccttgctgg gatcgatgat acccaccgag ccactgaacc agccatggtg
     3241 cgcactgccg gtgaacatga cccgcgaact cccgggaatg ctcctgacat ccttcaacag
     3301 atccggccac acgctctggt taccccacaa ggtgctggtg tgtgtgccat cggggttgat
     3361 ggtccagagc ccctgagccc gccacagagg tttgtcggtg tactcccatc tggtgtacat
     3421 cacacggcca tcactcgcca cggcggggag gtactccggc tcgacattcg gggaaaccag
     3481 atagatgtta cgaccgtccc cgtcacaccg ggccagcatg taggcgttag tagggggcat
     3541 acaccgaaca tacgcgttgc cgcgagtcgt gcagaacatg atgtggccgt cgggcaagta
     3601 gatggggtcc aggtcgtcat aggggccgtc cgtgagctgc ctcaagcccg tcccatcggc
//
LOCUS       DAOSPL010000284         3657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25381_length_3657_cov_2.658801, whole genome shotgun sequence.
ACCESSION   DAOSPL010000284 DAOSPL010000000
VERSION     DAOSPL010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25381_length_3657_cov_2.658801"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>549)
                     /locus_tag="PLQ54_06545"
     CDS             complement(<1..>549)
                     /locus_tag="PLQ54_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOS92954.1"
                     /translation="VPMRWGAIPANQTIDLAVRGMTCAHCARAVERALSGRVPGVSSA
                     SVNLATELVRVSYDPAAASVEAMASAVREAGFELVLPAFSDDDAEARRSETRALARQL
                     AVGMACTIPLVVLSMGRDGGLWGQWSHATWVNPLLLGLAIPVQFYTGWGYVTGAIRSL
                     RARQPNMDVLVALGSSAAFAYSA"
     assembly_gap    550..559
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            798..1427
                     /locus_tag="PLQ54_06550"
     CDS             798..1427
                     /locus_tag="PLQ54_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92955.1"
                     /translation="MQTGRLCEFALAALAIGASSEPQWPLDVETGLARNPHNRFGIPG
                     TTGTMVSVPDEFSTADSAFGRYRFTYSPAPGVSWSLSVAPPAFDSHDTAPKDISFLGS
                     ASTGSASAAGTALDISYVFNSCWLTYRRTFRPRGRLTWGPGLTAKVRDAEVHPTSDTQ
                     TASRGDLGVVPLTNFHADRWIGEDLSVVLGGDALAAPQGRAEHVMLALK"
     gene            1818..2348
                     /gene="idi"
                     /locus_tag="PLQ54_06555"
     CDS             1818..2348
                     /gene="idi"
                     /locus_tag="PLQ54_06555"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF002995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOS92956.1"
                     /translation="MGQHVVLVNAAGEDLGSMDVQAAHDGEGVRHRAFMVLIRNARGQ
                     VLLCRRSAHKRLWPRYWENSCSSHPMPGESHREAAERRLQEELGFTTALEPIGVFEYH
                     ALDGAAGAEHEVCAVLTGLHEGDVTPDSAEVEQCVWKDLSQLLTADDTDHWAPWLRPG
                     LEMLALKLSVNRQARG"
     gene            2355..2723
                     /locus_tag="PLQ54_06560"
     CDS             2355..2723
                     /locus_tag="PLQ54_06560"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS92957.1"
                     /translation="MRVAVIGGGLGGLAAAARLARAGLEVTVFEQNPAVGGKAAELRI
                     AGCRFDTGPSLLTMPQVVDDLFTDLGERREAHLRLRPLDVLCRYQFADGAVLRSCPDA
                     GAFGDRLAAATTDTAEAVVR"
     gene            2724..>3657
                     /locus_tag="PLQ54_06565"
     CDS             2724..>3657
                     /locus_tag="PLQ54_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS92958.1"
                     /translation="MARSETVYRLAADFFLYRPIAGWASFGSGISPAKGLWALRQLPR
                     LGFGQTMHGLHRRHFRDPRTIQLFDRYATYTGSDPHRCPATLALIPHVEHRLGACLVE
                     GGIYRLVEAVRDLALRQGVRLQTKTRVERIVTRNGRVRGVTAMGETFDFDAAVSATDM
                     NTTYRKLLADCPSGLGARQMRRPLSSSALVFYWAMRGEYPQLDIHNIFFSGDYRREFE
                     ELRQGACPVDPTVYVYRSCGYVRSDAPVGHENWHVMVNAPPDTGQDWRAEVERTRGRI
                     LSRLGSALDMDPALAIVEEGLLTPGRRGCRGGCRS"
BASE COUNT          604 a         1211 c         1267 g          565 t
ORIGIN      
        1 ggcggagtag gcaaacgcgg ccgaggagcc gagagcgacg agcacgtcca tgttcggctg
       61 gcgcgcgcgg agactgcgga tcgccccggt cacatagccc cagccagtgt agaactggac
      121 gggaatggcg aggcccagga gcagtgggtt cacccaggtg gcatgggacc actggcccca
      181 caggccgcca tcgcggccca tgctgaggac gacgaggggg atggtgcatg ccatgccgac
      241 ggcaagctgg cgtgcgaggg cgcgcgtctc actccggcgc gcctcggcat cgtcgtcact
      301 gaacgcagga agcaccagct cgaacccggc ttcgcgcacg gcgctggcca tggcctccac
      361 actggcggca gccgggtcgt atgagacgcg cacgagctcg gtggccaggt tcaccgaggc
      421 ggaggagacg cccggcacgc ggccactgag cgcgcgttcg actgcgcgag cgcagtgggc
      481 gcaggtcatg cctcgcaccg caagatcgat ggtctggttg gccggaatcg ccccccaccg
      541 catcggcacn nnnnnnnnng gcaccactat ccagtatcaa catgcgggag gccgcaaacg
      601 actccgcccc ggaccgcggg cacgcggcga ggcgccgtgg aggaaccacg ggcaatgccg
      661 gtggacctga aacgggtctc gcatgcccgg cgcgcgcagg tgaaacctcg gttgcaggga
      721 atgggcccat tgccacagag gcgccgcaga gggccccggt gggtgcggac tgccgctgcg
      781 agcgaaggag acccgacatg cagacaggca ggctctgtga gtttgccctt gcagccctcg
      841 ctattggtgc atcgtccgag cctcaatggc ccctcgacgt ggagacggga ctggctcgga
      901 acccgcacaa ccgcttcggc atccccggca ctacagggac gatggtgtct gtacccgatg
      961 agttcagcac tgctgacagc gcattcggcc ggtaccggtt cacctactcc cccgcccccg
     1021 gggtcagctg gtcactgtcg gtcgccccgc cggccttcga cagccacgac acggccccga
     1081 aagacatcag cttccttggc tccgcctcca ctggctccgc ctccgcggcc gggacggccc
     1141 tcgacatcag ctatgtcttc aactcgtgtt ggctcaccta ccggcgcacg ttccgcccca
     1201 ggggccggct gacctggggc ccgggtctga ccgccaaggt acgagatgcg gaggtacatc
     1261 cgaccagcga tacccagacg gccagccggg gagaccttgg agtggtgccg ctgaccaact
     1321 tccacgctga ccggtggatt ggcgaagacc tgtctgtggt gttaggcggc gatgccctgg
     1381 cggctcccca ggggcgagcc gagcacgtca tgctcgccct gaagtagtag ctttcgccca
     1441 cgacccgggt gcgtctgggc taccgcatac tcgagggcgg tgtggacgac gactcggtct
     1501 acagcttctc gcgcgtcgac tatgcgttgg tcggggtcga gtacggattc tgaacgacgc
     1561 caacgcaacg gcggcggacc tgcagaagct cacatgggtc tctggaagcg gggcgagaca
     1621 tcactcctcc tgccggccac gagctgttcg gccggcagga gatttgtcgg gattgcggcc
     1681 cgggccaact ggatccccgc cgcccgaagg gcccggcaca cagccggacc aaacggcctg
     1741 ggaatccccg tcatgccgtg actcaccagc cccgacagcg ggctgcctga cggacttgca
     1801 cagcagggag gaactagttg ggtcagcacg tggtactcgt caacgcagcg ggggaagacc
     1861 tgggcagcat ggatgtccag gccgcccatg atggtgaggg cgtgcgacat cgtgccttca
     1921 tggtgctgat ccggaacgca cggggacagg tcctgctctg ccggcgcagc gcgcacaagc
     1981 gcctgtggcc gaggtactgg gagaacagct gctcgagcca ccccatgccg ggcgagagcc
     2041 accgcgaggc tgctgagcgc cggctgcagg aggagctggg cttcaccact gcgctggagc
     2101 ccatcggggt gttcgagtac cacgcgctgg atggcgctgc gggagcagag cacgaggtct
     2161 gcgcggtcct gaccggtctg cacgagggtg acgtgacgcc ggacagcgcg gaagtcgagc
     2221 aatgcgtctg gaaggacctg agccagctgc tgacggcgga cgacaccgac cactgggccc
     2281 catggttacg gcccgggctg gagatgctcg ccctgaagct ttccgtcaat cggcaggcca
     2341 gggggtgaac gcccttgagg gtcgcggtga tcggtggtgg tctggggggg ctggcggctg
     2401 cggccagact ggcccgggcc ggcctggagg tgacggtctt cgagcagaac ccggccgtcg
     2461 gtgggaaagc cgcggagttg cgcattgctg gttgccgctt cgacaccggc ccctcgcttc
     2521 tgacgatgcc gcaggttgtg gacgacctgt tcaccgacct cggagaacgc cgcgaagcgc
     2581 acctccgcct gcgacctctg gacgtgcttt gccgctacca gttcgcggac ggggctgtct
     2641 tgcggagttg ccctgacgca ggcgcctttg gggacagact ggccgccgcg actaccgaca
     2701 ccgcggaggc tgtcgtccgg taaatggccc gcagcgagac ggtctaccgg cttgccgcgg
     2761 acttcttcct ctatcgtccg atcgccggct gggcgtcctt cgggagcggc atctctccgg
     2821 ccaaaggcct gtgggccctg cgccagttgc cccgcctggg attcggccag accatgcacg
     2881 gccttcaccg caggcacttc cgggaccccc gcacgatcca gctcttcgac cgctacgcca
     2941 cctacacggg ctccgacccg caccgctgcc cggctaccct ggcgctgatc ccccatgtgg
     3001 agcacaggct gggcgcctgc ctggtggagg gaggcatcta ccggctggtg gaggcggtgc
     3061 gcgatctggc gctgaggcaa ggtgtgagac tgcagaccaa gacccgagta gagcgcatcg
     3121 tgacgcggaa tgggcgggtg cgtggcgtca ccgccatggg agagaccttc gacttcgatg
     3181 ccgcggtgtc ggcgactgac atgaacacga cgtaccggaa gcttctggcc gactgtccca
     3241 gcggcttagg ggcgcgccag atgcggcggc ccctgtccag ctctgccctg gtgttctact
     3301 gggcgatgcg gggtgagtac ccccaactcg acattcacaa catcttcttc tcgggcgact
     3361 accggcgcga gttcgaagag ctgcgccagg gagcttgccc agtggacccc accgtctacg
     3421 tgtaccgctc gtgcggctac gttcgctccg atgcgccggt cggccacgag aactggcatg
     3481 tcatggtcaa cgccccaccc gacaccggcc aggactggag ggcggaggtc gagcgcacgc
     3541 gaggccgcat cctcagccgc ctgggcagtg cactggacat ggaccccgct ctggcgattg
     3601 tcgaagaagg gcttctgacg ccgggccgta gaggctgccg cggtggctgc cggtccg
//
LOCUS       DAOSPL010000285         3653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25418_length_3653_cov_3.145914, whole genome shotgun sequence.
ACCESSION   DAOSPL010000285 DAOSPL010000000
VERSION     DAOSPL010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25418_length_3653_cov_3.145914"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PLQ54_06570"
     CDS             <1..792
                     /locus_tag="PLQ54_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92959.1"
                     /translation="IHELADPRVTAVQEAYVREVIRTVNDLDNVLFEISNENHPPSTD
                     WQYHMIELIRRTERAMPKQHPVGMTFQYQGGSNQTLFDSPADWISPNPDGGYRDDPPA
                     ADGTKVIINDTDHLWGIGGNAQWVWKSFCRGLNPIFMDPYDGAVIGGRFEPRWDPVRK
                     SLGQALALAERLDLARMTPHNALASTGFCLADPGVDYIVYLPDGGVAELDLSAAQGGL
                     SVEWIDASTGAATPGASVDGGGRIRLAAPFAGNAVIHVWVPPAER"
     gene            complement(876..1427)
                     /locus_tag="PLQ54_06575"
     CDS             complement(876..1427)
                     /locus_tag="PLQ54_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92960.1"
                     /translation="MRATAGVVLVLVALGSCVLRGARAQEVTAVDGIWPATNAPWPND
                     PIDRARAAQHEADRRAGGKHDGSTVDGIWPATNAPWPNDPIDRARAAQHEAARVRRGY
                     GATQQYYRGLEWYREYMLSNGLPYPTDGFVVVRPEWGYRFPGSSLQGWPYSQLPIDHY
                     SGYRYPLRYEPGGWPYYGPGWSR"
     gene            1714..2577
                     /locus_tag="PLQ54_06580"
     CDS             1714..2577
                     /locus_tag="PLQ54_06580"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOS92961.1"
                     /translation="MLATRALTALVGLPAGIALMYLGGWYWMAVIAVMMIKGQHELLS
                     AARKRGCQPCDALVYVFGLSLLLTVQAAPGNWELRAVLSIVFVMAIMTLEVFRKSQTH
                     FANAGVALLAFFYIAWLMGYLVLMQRLEASPSLERPSWPWTMPFGFSVLLYTFGVTWI
                     TDTGAYLVGKNLGRSKLCPSISPGKTVEGSYGAFLTAIVASAVLGTGFIGLPIAHAVL
                     LGVIMGVLGQLGDLAKSVLKREAGIKDFGAVFPGHGGVLDRFDAVLFNAIIIYYYVAA
                     LNPAAAVASSL"
     gene            2617..3102
                     /locus_tag="PLQ54_06585"
     CDS             2617..3102
                     /locus_tag="PLQ54_06585"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOS92962.1"
                     /translation="MHELALRYVEALPLPPAALVCLASMVPVIESRYAIMFACALGIP
                     FAQALALTTAGNTLPMVPVLLLLGPISDRLRVWPVADRFFTWLFARARKRRDLIDRYG
                     PLGLALFVAVPLPGSGGYSGAILAFVLGLRFWPSLIAIIAGILASAIACGAGAYGLAS
                     L"
     gene            complement(3081..>3653)
                     /locus_tag="PLQ54_06590"
     CDS             complement(3081..>3653)
                     /locus_tag="PLQ54_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HOS92963.1"
                     /translation="WQADGAAQSAQPAFAPPPPVPAGGMEWTAPSERDGGGAPYSVWA
                     IVAFASVFLCPCLLIQGPMALVALAEISNKGMRGRGLAIAALICSLFLTVATVFVVVA
                     AVHGAQRQAEELQEQFEGLTDMDVYEQDEVAPGSQGFTAPGGPQMPGGFPGPAVQPPR
                     SMPGMNYQGGWLGLPGGAEALRRPYRLASP"
BASE COUNT          542 a         1292 c         1177 g          642 t
ORIGIN      
        1 atccacgagc tggccgaccc tcgggtcacc gcggtccagg aagcatacgt gcgcgaggtg
       61 atccgcacgg tcaacgatct cgacaacgtg ctcttcgaga tctccaacga gaaccatccc
      121 ccatccaccg attggcagta ccacatgatc gaactgatcc gtcgcaccga acgcgccatg
      181 cccaagcagc acccggtcgg gatgaccttc cagtatcagg gcgggtcgaa tcagacgcta
      241 tttgatagtc ccgccgactg gatctcgccc aaccccgatg gcgggtaccg cgatgacccc
      301 ccggccgctg atggcaccaa ggtcatcatc aacgacaccg atcacctgtg gggcatcggc
      361 ggcaacgcgc agtgggtgtg gaagagcttc tgccgtggcc tcaacccgat cttcatggat
      421 ccctacgacg gggcggtgat cggggggcgc ttcgagcccc ggtgggaccc ggtccgcaag
      481 agcctgggcc aggccctggc cctcgccgag cgcctcgacc tggcccggat gacgccgcac
      541 aacgccctcg cctcaaccgg cttctgcttg gccgaccccg gcgtcgatta catcgtctat
      601 ctgcccgatg ggggcgttgc cgagctcgat ctctccgctg cccaaggcgg cctttcagtc
      661 gagtggatcg acgcctcgac cggcgcagca acccccggcg cctccgtcga cggcggtggg
      721 aggattcgac tcgcggcccc cttcgcgggg aacgcggtca tccatgtctg ggtccccccg
      781 gccgagcgct aggcggccgg tgatgagccg tggccgcggt cccaggcgag gtcagggagt
      841 ccggtttcgg accgccgcgt cgtgcatgcc ctcgactagc ggctccaacc aggtccgtag
      901 tagggccagc cgcccggctc atacctcaac gggtaccggt agccgctgta gtggtcgatc
      961 ggcagctgcg agtatggcca accctgcaac gacgacccgg ggaatcggta gccccattcg
     1021 ggcctcacga ccacaaagcc atcggtcggg taggggagtc cgttcgagag catgtactcc
     1081 cggtaccact ccaagccccg gtaatactgc tgcgtcgccc catagccgcg acgcacacgg
     1141 gcagcctcat gctgggcggc ccgtgcccgg tcgatcgggt cgttgggcca cggcgcgttg
     1201 gtggcgggcc agatgccgtc cacggtggat ccatcatgct tccccccggc gcggcggtcg
     1261 gcctcgtgct gggcagcccg cgcccggtcg atcgggtcgt tgggccacgg cgcgttggtg
     1321 gcgggccaga tgccgtccac ggccgtcacc tcttgagccc gagcaccccg gaggacacag
     1381 gacccgagag ccaccaacac caacacgact ccggctgttg cccgcatggg acatgccacc
     1441 tccccaggcg ccgccggttc acgcgacacc atccggccgc gagaacggtc ggtaccctat
     1501 caccttggac tgtgtacatc ggtcgcgggt tcccatggaa tgcacgggcg cttcggggcg
     1561 gcgccgcggc ccagtgattc ccctcgaggg gcgaccgccc tgctgggtgg cgggactcgg
     1621 aggcacgggc aggggacctg gagcctcgcg ctgaagctta tgcccgtcag gtagcgtcgc
     1681 cgctggcaat ctgacacgag cgggaggcgt cgcatgctcg cgacacgcgc gctcacggcg
     1741 ctggtcggcc tgccggccgg tattgccctc atgtacctcg gcgggtggta ctggatggcc
     1801 gtcatcgccg tgatgatgat caagggccaa cacgagttgc tgtctgcagc ccgcaagaga
     1861 ggctgccagc cctgtgatgc attggtctat gtcttcgggt taagcttgct gctgacagtc
     1921 caggcggctc ccgggaactg ggagctccgg gcggtcctgt ccatcgtgtt tgtcatggcg
     1981 atcatgaccc tcgaagtctt ccgcaagtca cagacccact ttgcgaatgc gggcgttgcc
     2041 ctgctggcgt tcttctacat cgcctggctc atggggtacc tggtcctcat gcagaggctg
     2101 gaggcgtcgc catcgctcga acgtccctca tggccttgga ccatgccctt tggtttctca
     2161 gtgctcctct ataccttcgg cgtcacctgg atcaccgaca cgggcgccta tcttgtgggc
     2221 aagaacctgg gccgcagcaa gctatgccct tccatcagtc ctggcaagac ggtcgaggga
     2281 agctatggcg ctttcctgac cgcgatcgtc gcttccgcag tgctggggac cggtttcatc
     2341 gggctgccca tcgcccacgc cgtgctgctc ggcgtcatca tgggcgtcct cggccagctg
     2401 ggtgatctgg ccaagtcggt tctcaaacgc gaggccggta tcaaggactt cggtgccgtc
     2461 ttccccggcc atggcggggt gctcgaccga ttcgacgccg tgctgttcaa cgccatcatc
     2521 atctactact atgtggcggc cctgaacccc gcggcggctg tggcatcctc gctctagtca
     2581 gtccgcggcc cgtgtgcccc ggaggccccc cagtccatgc atgagttggc gctgcgctat
     2641 gtcgaggccc tgccgctgcc gccagcggcg ctggtatgcc tcgcgtcgat ggtccccgtg
     2701 atcgagtccc gctatgccat catgttcgcc tgcgccctgg ggatcccgtt cgcccaggcc
     2761 ctggcactca ccaccgcagg caacacgctg cccatggtgc cggtactgct gctgctgggc
     2821 ccgatctccg atcgattgcg cgtctggcct gtggcggatc gattcttcac ctggctgttc
     2881 gcgcgcgccc ggaagcgtcg cgatctcatc gaccgctatg gcccgctggg gctcgccctc
     2941 ttcgtcgccg tccctcttcc gggctccggg ggctacagcg gcgccatcct ggctttcgtc
     3001 ctcggcctcc gattctggcc atcgctgatc gccatcattg cggggatcct cgcctcggcc
     3061 atcgcctgcg gcgcgggcgc ctacggactc gcgagtctgt agggccgccg gagcgcttcc
     3121 gccccacccg gaagacccag ccacccgccc tggtagttca tgcccggcat cgatcgtggc
     3181 ggctgcacgg cggggcccgg gaagcccccg ggcatctgtg ggccgcccgg agccgtgaag
     3241 ccttgcgagc ccggcgcaac ctcatcctgc tcgtagacgt ccatgtcggt caggccctcg
     3301 aactgctcct gcagctcctc ggcctggcgt tgggcgccgt gcacggccgc caccaccaca
     3361 aagacggtgg cgaccgtcag gaagaggctg cagatgagtg ccgcgatggc gaggcctcgc
     3421 ccgcgcatgc ccttgttcga gatctcggcc aacgccacca gcgccatggg gccctggatg
     3481 aggaggcacg ggcagagaaa caccgacgcg aacgccacga tggcccacac cgagtagggc
     3541 gcgccgccgc cgtcgcgctc ggagggggct gtccactcca tgccaccagc cggtaccggc
     3601 ggcggcgggg cgaacgctgg ctgtgcggac tgggcggcgc catccgcctg cca
//
LOCUS       DAOSPL010000286         3644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25502_length_3644_cov_4.231262, whole genome shotgun sequence.
ACCESSION   DAOSPL010000286 DAOSPL010000000
VERSION     DAOSPL010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25502_length_3644_cov_4.231262"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /gene="thyX"
                     /locus_tag="PLQ54_06595"
     CDS             complement(<1..756)
                     /gene="thyX"
                     /locus_tag="PLQ54_06595"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOS92964.1"
                     /translation="MQPVTARVTLVRYTPDAELVVAAAARLCYAAETDGLMAQDPEGV
                     RRLVARLRRMGHLSPIEHVGFTFYLEGVSRAMSHQLVRHRIASYSQRSQRYVDHDGFD
                     YVVPPQLAGHVVDTPGGPVPATEYFIETMGLIAARYAALNDALGRRGEASNEDARYVL
                     PNACETRLFVTMNGRELLHFFEERLCLRAQWEIRDVAGRMLDCVREVCPAVFAGVGPK
                     CVRRGGCPEGKMSCGRYIEMLERYGDESGGAHDA"
     gene            complement(816..1637)
                     /locus_tag="PLQ54_06600"
     CDS             complement(816..1637)
                     /locus_tag="PLQ54_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92965.1"
                     /translation="MLLATTGIALALCGAEGAEALITPPTDYGRSYVTTAGGAGNEPV
                     FWLESRCRISDPATGEVRDYYQCASCKSEDTFATHDLFLDPNYDFLPVFSGTESVIFR
                     RRLPAAEEYRQVVPDSPWGAITPLLRTAQMRVLTTPDEVVAAIGAAVPIVCQTELRDE
                     VSGRTAVIEYPVKTMNMNSIRRIYQVDTGPVLLPDLTVPSDQWSASLRLAFIAFNEDG
                     WADFVVDEPTPAGGEVKVHHFSRRLHFAARNVILALDDGSLPWEAPARAAKAAGD"
     gene            1768..>3644
                     /locus_tag="PLQ54_06605"
     CDS             1768..>3644
                     /locus_tag="PLQ54_06605"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOS92966.1"
                     /translation="MATRLLAGAVSALYLITPGLCAAEGDEAADVLAGLRPEHPRIML
                     TDERLAELKARAAEDSPLRRYVDDVLTAANGLLEADELRHVLPDGIRLLSVSRACLSR
                     VSTLALAYRWTSNERYLSAALRDLRSVCAFKDWNPDHFLDTAEMTHAVAIGYDWLYAA
                     LDDETRQILRAAILHLGLEPGLRGYRGEKYSFPRAENNWNQVCNSGLTVGALAVADTD
                     PDVAREIIACAIASLPTAMRHYAPDGAWMEGPGYWDYATSYICYGIAALDSALGRDLG
                     TSEYPGLADSGYFPIYGAGPTGCYLSYADAGMMSRATPKACLFFLAGKFGRPEFADAE
                     HAILRDQTASAFHVVWYQPPSGQTFERDLDRLFRGPVEVAFLRSAWGDPSALWVGAKA
                     GYNQVPHGHLDLGNFELDALGVRWAIDLGADDYNLPGYWSGREDGQRWTYYRLNSQSH
                     NVCTIDGNNQLVNARAQIVRWQSRPESGAVVIDLTNAYAHVERAMRGVQLLRREGAIL
                     IQDEFTLAGPRNLVWGMTTRAEITVDDDGSARLAQDGQQLRVRVLEPAGAGFEEGSAQ
                     RDRPEAENKGFRRLLLPLRNATGDQRIALLFVPVTADAQGEPRLTIVPLADWSGDAAN
                     DP"
BASE COUNT          604 a         1262 c         1197 g          581 t
ORIGIN      
        1 ggcatcgtgt gcgccgccgg actcgtcacc gtagcgttcc agcatctcga tgtaccgtcc
       61 acagctcatc ttgccctcgg ggcagccacc gcgccgcaca cacttggggc cgactccagc
      121 gaagaccgcg gggcagacct cccgcacgca atccagcatc cgaccggcta cgtctcggat
      181 ctcccactgc gcgcggaggc acagccgttc ctcgaagaag tgcagtagct cccgcccgtt
      241 catagtcacg aacagccgcg tctcgcaggc attgggcagg acatagcgcg cgtcttcatt
      301 gctggcctcg cctcgcctcc cgagggcgtc gttgagcgcg gcatagcgtg cggcgatcag
      361 ccccatggtc tcgatgaagt actcagtggc cggcaccggg ccgccgggcg tgtccaccac
      421 atggcccgcc agctgggggg ggacgacata gtcgaatccg tcgtggtcca cataacgttg
      481 gctgcgctgg gagtagctgg cgatgcggtg ccggaccagc tggtggctca tcgcccgcga
      541 cacaccctcc aagtagaacg tgaacccgac gtgttcgatg gggctcagat ggcccatccg
      601 cctgaggcgc gccaccaacc ggcggacgcc ctcagggtcc tgcgccatga gaccgtcggt
      661 ttcagcggca tagcacagcc gggcggcggc ggcgacgacc aactcggcgt cgggtgtgta
      721 gcgcaccagt gtgactcgcg ccgtaacggg ctgcacgtag cctcaacccc tcgtggtgtg
      781 tccgggcccg ctgatcgccc aggatcggtg gcccctcaat cccctgcggc cttggcggca
      841 cgagccgggg cctcccaggg cagagaccca tcgtccaggg ccagaatcac gttccgcgcg
      901 gcgaagtgca accgcctgga gaagtgatgg accttcacct cgccaccagc cggcgtgggc
      961 tcgtcaacca cgaagtcggc ccacccgtcc tcgttgaaag cgatgaaggc caaccgtagg
     1021 ctggccgacc actggtccga cggcaccgtc aggtcgggca gcagcaccgg gccggtgtcg
     1081 acctggtaga tccgccggat ggagttcatg ttcatggtct tcaccggata ctcgatgacc
     1141 gccgttcggc ccgagacttc gtcgcggagt tccgtctggc acacgatggg taccgccgcc
     1201 ccaatggccg ccacgacctc gtcgggggtg gtcagcacgc gcatctgggc ggtccgcagg
     1261 aggggcgtga tcgcgcccca gggcgagtcc ggcaccacct gccgatactc ctcggcggcc
     1321 ggtaggcggc ggcggaagat gaccgactcg gtcccgctga agaccggcag gaagtcgtag
     1381 ttcgggtcca ggaataggtc gtgggtggcg aaggtgtcct cgctcttgca cgaggcgcac
     1441 tggtagtagt cgcgcacctc gccggtcgcg ggatcgctga tgcggcagcg cgactcaagc
     1501 cagaacaccg gctcgttgcc cgcgcctcca gcggtggtga cgtaggagcg gccgtagtca
     1561 gtcggtggtg tgatcagcgc ttcggcgcct tcggcgccgc acagggcgag tgcgatgccc
     1621 gtggttgcga gtaacatgcg acgacctccc acagccgtaa cgtcactgtg tctaacgcca
     1681 agcgagggga aggatacggc agccaccgaa tcggtggcaa tgcgggaggc ccgtccgagg
     1741 cccgcgacag gaggtctggc cacgacgatg gcaactcgac tactggccgg ggctgtatcg
     1801 gcgctctact tgataacccc gggcctgtgc gccgccgagg gcgatgaggc ggcggacgtg
     1861 cttgcggggc tccgcccgga gcacccgagg atcatgctca ccgacgagcg gctggcggag
     1921 ctcaaagccc gcgcggccga ggactctccg ttgcgacgct atgtcgatga cgtgctcacc
     1981 gccgccaatg gtctgctcga ggccgacgag ctccgccacg tcttgcccga tggcatccgg
     2041 ctcctgagcg tcagccgcgc gtgcctgagc cgtgtgagca cactcgccct ggcctatcgc
     2101 tggacgagta acgagcgcta cctcagcgcc gcgctccgcg acctgcgttc ggtctgcgcc
     2161 ttcaaggact ggaaccccga ccacttcctc gacacggccg aaatgaccca cgccgtcgcc
     2221 attggctacg actggctcta cgccgccctc gatgacgaaa cacgccagat tctccgcgcg
     2281 gcgatcctgc acctcggcct cgagcccggg ctacggggct atcgcggcga gaaatacagc
     2341 ttcccccgcg ccgagaacaa ctggaaccaa gtgtgcaact ccggcctgac cgtgggggca
     2401 ttggccgtcg ctgacactga ccccgacgtc gctcgcgaga tcattgcttg cgccatcgcc
     2461 tcactcccca cggccatgcg gcactatgcc ccggatggtg cctggatgga gggcccgggc
     2521 tactgggact acgcgacgag ctacatctgc tacggcatcg cggcactcga tagcgccctg
     2581 ggccgcgacc tgggaacgtc cgagtatccg ggtctggcgg actcgggcta cttccccatc
     2641 tacggcgccg gacccaccgg ctgctatctg agctatgccg atgcgggcat gatgtcgcgc
     2701 gccacgccca aggcctgcct gttcttcctg gccggcaagt tcggacgccc cgagttcgcc
     2761 gatgccgaac atgccatctt gcgcgaccag acggcgagcg cgttccatgt cgtctggtac
     2821 cagccgccct ccggccagac cttcgagcgc gatctcgacc gcctcttccg agggccagtc
     2881 gaggtggcgt tcctgcgctc ggcgtggggc gaccccagcg ctctctgggt gggcgccaag
     2941 gcgggctaca accaggtccc gcacgggcat ctcgatctcg gcaacttcga gctcgatgcg
     3001 ctcggcgtgc gctgggccat cgaccttggt gccgacgact acaacctgcc agggtactgg
     3061 agcggccgcg aagacgggca gcgctggacc tactatcgcc tgaactctca gagccataac
     3121 gtctgcacca tcgacggcaa caaccagttg gtgaatgcaa gggcgcagat cgtccgctgg
     3181 cagagtcgcc ccgagtccgg cgcggtcgtc atcgacctga cgaatgccta cgctcacgtt
     3241 gagcgtgcca tgcgcggggt ccagctcttg cgccgagaag gtgcgatcct catccaggat
     3301 gagttcacgc tcgccgggcc tcgcaacctc gtgtggggaa tgaccactcg agccgagatc
     3361 acggtggacg acgacggttc ggcacggctg gcccaggacg gccagcaact ccgtgtgcgc
     3421 gtgctcgagc ccgcgggagc gggcttcgag gagggttcgg cccagcgcga taggcccgag
     3481 gctgagaaca agggcttccg gcggctgctc ctgccgctca ggaacgccac cggcgaccaa
     3541 cgcatcgcgc tgctcttcgt acccgtaacg gccgacgccc aaggggagcc gcggctcacc
     3601 atcgttccgc tggccgactg gtcgggcgac gcggcgaacg atcc
//
LOCUS       DAOSPL010000287         3643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25520_length_3643_cov_2.741639, whole genome shotgun sequence.
ACCESSION   DAOSPL010000287 DAOSPL010000000
VERSION     DAOSPL010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25520_length_3643_cov_2.741639"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PLQ54_06610"
     CDS             complement(<1..393)
                     /locus_tag="PLQ54_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOS92967.1"
                     /translation="MFQRILVPMDVSPVDQTVLPHVRALAGAFGSEIILLRVAHYHTR
                     DAREHEVTEAQGYLQTIADALASDGFRVRHLVEHGEPVDAIIGTAEREGADLIAMAGH
                     GHRHMVGWFLGSTIEDVRHRCCVPLLLVR"
     gene            complement(432..1757)
                     /locus_tag="PLQ54_06615"
     CDS             complement(432..1757)
                     /locus_tag="PLQ54_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOS92968.1"
                     /translation="MDPASTVAAAAPERTWRRQMAARSPVARALGRFARSPILRYLGP
                     GFIVTVGFIDPGNWATNIAGGSEFGYTLLWVITLSTAMLILLQSMSARLGVVTGHSLA
                     ANIRSRAPRWVSHSLGATVIVACAATDVAEYLGAALGFHLLFGLPLWLGAIVTVAIVV
                     TAILGQRYHSLERMILVFLAVIAGCYLIELWIVKPNWGTLAPCVVTPRIDSRSILVAM
                     GMLGAVVMPHNVFLHSNVIQSREWGDDDASKRRLIRFELVDTIVAMGMGWVVNSSMII
                     VAAAVFFQHGTVVRSIEEASRTLQPLAGPLAQVLFGVALLFAGVGSSITSSLAEANVV
                     CGFLGKPEDTHSRFYRLGLVVTSVPAMVVIAAGFDSYKTLILSQVALSLQLPFTIIPL
                     LILARSRKVMGSFRSGPGEFLLAVIVAGAVMVLNALLLYQTFGGTFDFG"
     gene            1934..>3643
                     /locus_tag="PLQ54_06620"
     CDS             1934..>3643
                     /locus_tag="PLQ54_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92969.1"
                     /translation="MSRQPRVAPLLRSAWLIAAPWIVLLTPGLGGAQEVFTVNSFESE
                     DELASWQITCAGTRLVQEGVTHGHHALEMTFDPNGEWAAVSMYWNRVVRDWSPYDALV
                     IDVLNPNDGPISGYELVADEAWRDSGGSYWNRHNGNVTFAPGRGQWVIPVRGLYRGEA
                     GSRNNDIKTDIDPARIVRLDLNFGSRGQTGRIVIDHIRFVKASRPASVQAFDFGPPSQ
                     PVMLGWTPVSHETAYTAEQGYGWGPQGGAPWDGADRDTTFGTMLTQDFCEAGGYRFHV
                     DVPPGQYEALVIFENCGYWGGEQARCSERSISADGELAWSERRPHGRSTALFRFEDVE
                     PLGRDLWTTYMAEEITRPVRFTVAAGADGITLGFASDAPWGAKLSALALYPSADAAAR
                     EWVDGQWKAVENEFRAKAVCLDAPGPAFDAPREWVPLGLVAWPVSLDDDLSPSAVPEA
                     PVGPGDLKLQRHAMRGEFEPFCLALRPQQDLGRCALSIEPDGPALPATASVVWYNTSR
                     DFGSMAYHVVPHTLRPQDAIDLPAGITREVVVTVHVPEDAAPGEYRFRLRLRTPGGRE
                     LLSV"
BASE COUNT          633 a         1225 c         1219 g          566 t
ORIGIN      
        1 ccgcacgagc agcaggggca cgcagcagcg gtggcggacg tcctcgatgg tgctcccgag
       61 gaaccatcca accatatgcc ggtgaccatg gccggccatg gcaatgaggt ccgcgccctc
      121 gcgctcggcc gtgccaataa tggcgtcgac cggctcgccg tgctcgacaa ggtgcctgac
      181 cctgaagccg tcactcgcca gcgcgtcggc gatggtctgc aggtacccct gggcctcggt
      241 cacctcgtgt tctctcgcat ctcgggtatg gtagtgggcc actcgaagca ggatgatctc
      301 gctgccgaag gcgccggcca gggctcgcac gtgcgggagc acggtctggt cgaccggtga
      361 tacgtccatc ggtaccagga tcctctggaa catggcgcac ctccctctcc cgggagcggg
      421 cagtccggcc gctagccgaa gtcgaacgtg cccccgaatg tctggtagag cagcagggcg
      481 ttgagcacca tcacggctcc agcgacgatg acggccagga ggaactcccc cgggccgctc
      541 cggaagctgc ccattacctt gcggcttcgc gccaggatca gaagcggaat gatggtgaac
      601 ggcaactgca ggctcagcgc gacctgactg aggattagcg tcttgtagct atcgaacccg
      661 gcggcaatca ctaccatggc cggaaccgac gtaaccacca ggccgaggcg gtagaagcgg
      721 ctgtgggtgt cctccggttt ccccaggaac ccacacacga cgttggcttc ggctagcgac
      781 gacgtgatgg acgagcccac gcccgcgaac aggagggcga cgccaaagag cacctgagcc
      841 agagggcccg caagcggctg gagggtccgg ctggcctcct cgatactccg caccacggtc
      901 ccatgctgga agaacacggc cgcggccacg atgatcatgg aggagttgac gacccagccc
      961 attcccatgg ctacgatggt gtccacgagc tcgaaccgaa tcagccggcg cttcgaggca
     1021 tcgtcgtcgc cccactcgcg gctctggatc acgttggagt ggaggaacac gttgtggggc
     1081 atcactacgg cgccgagcat tcccatggca accagaatgc tcctggagtc gatgcgtggc
     1141 gtgaccacac acggcgccag cgtgccccag ttgggcttca cgatccagag ctcgatgagg
     1201 tagcagccag cgatgacggc caggaagacg aggatcatac gctccagcga gtgatacctc
     1261 tgcccgagga tcgccgtcac cacgatagcc acggtgacaa tggccccgag ccagagcggg
     1321 aggccaaaga gcaagtggaa acccagagcg gcccccaggt actcggccac gtccgtggcg
     1381 gcgcaggcca cgatcacagt cgcgccaagg gaatgagaga cccagcgcgg cgcgcggctg
     1441 cggatgttcg ccgccagcga gtgcccggtg accacgccga ggcgggccga catgctctgg
     1501 agcaggatca gcattgcggt gctgagcgtg atgacccaga gcagtgtgta gccgaactcg
     1561 gaccccccgg cgatgttcgt ggcccagttg ccggggtcga tgaaccccac ggtcacgatg
     1621 aaaccgggac ccaggtagcg gagaatgggt gacctggcga accgcccgag cgcccgtgcc
     1681 acgggcgacc tcgccgccat ctgtcggcgc caggtgcgct cgggggctgc agcggccacg
     1741 gtcgaggctg ggtccatcaa cgccctccgt cgggtgccct ggtcacgggt cgatgtagac
     1801 cctcagcttc ggcggtccgc cgcgcggcgc cttcagcgct gaggcaggtc ccagaccctc
     1861 ggtcgagcga acacgccccc gttcggcgga tgcgggcccc agagccctca cgtcacagtg
     1921 caggaggcgg accatgtcgc gccaaccgcg ggtcgccccg ttgctccgtt ccgcctggct
     1981 gatcgccgct ccctggatcg tcctgctgac cccagggctc ggaggagcgc aggaggtatt
     2041 caccgtcaac agcttcgagt ccgaggacga gcttgcgagc tggcagatca cctgtgccgg
     2101 cacacgtctc gtccaagagg gggtcacaca cggccaccac gccctcgaga tgaccttcga
     2161 tcccaacggc gagtgggcgg ctgtcagcat gtactggaac cgagtggtcc gcgattggtc
     2221 gccctatgat gccctggtca tcgacgtact caacccgaac gacgggccca tcagcgggta
     2281 cgagttggtg gccgatgagg cgtggcgcga ctcgggaggg tcctactgga accggcacaa
     2341 cggcaatgtc actttcgcgc ccggacgcgg ccagtgggtc atccccgtcc gcgggctcta
     2401 ccggggtgag gccggtagcc gcaacaacga catcaaaacg gacatcgacc cggcccgcat
     2461 cgtccggctc gatctgaact tcggcagtag gggccagacc ggccgcatcg tcatcgacca
     2521 catccgcttc gtgaaggcta gccggcccgc gagcgttcag gcgttcgact tcggacctcc
     2581 gagccagccg gtgatgctcg gatggacgcc agtttcccac gagaccgcct acacggccga
     2641 acaaggctat ggttggggcc cgcaaggtgg cgctccgtgg gacggggccg atcgcgacac
     2701 caccttcggc accatgctga cccaggactt ctgcgaggcc ggcgggtatc ggttccatgt
     2761 cgacgtgccg ccgggccaat acgaggccct cgtgatcttc gagaactgcg gctactgggg
     2821 cggcgagcaa gcgcgctgct ccgagcgctc gatctcggca gacggcgagc tggcatggtc
     2881 ggagcgacgg ccccatggtc gtagtacggc tctgttccgg tttgaggacg tcgagccact
     2941 cgggcgcgac ctgtggacga cctacatggc tgaggagatc acccgacccg tacgcttcac
     3001 tgtggctgcg ggcgccgatg gcatcacgct ggggttcgct tcggacgcgc catggggcgc
     3061 caagctctcg gccctggcac tgtacccttc agccgacgcg gcggccagag agtgggttga
     3121 tggccagtgg aaggccgtcg agaacgagtt ccgcgccaag gccgtgtgtc tcgacgcgcc
     3181 cggcccagcg tttgacgcac cgcgcgagtg ggtaccgctc ggcctggtcg cttggccggt
     3241 ttcgctggat gacgacctgt cgccctcggc ggtgccggag gcgcccgtcg gccccggcga
     3301 cttgaagctg cagcgccatg ccatgcgtgg cgagtttgag ccgttctgcc tggccctccg
     3361 gccccagcag gacctgggcc ggtgcgcact ctccatcgaa cccgatggcc cggcgttacc
     3421 cgccaccgcc agtgtggtct ggtacaacac cagccgggac ttcggcagca tggcttacca
     3481 cgtcgtgccg cacacgctga ggcctcagga cgcgatcgac ctccctgccg gcatcacgcg
     3541 tgaggtcgtc gtcaccgtcc acgtgcccga ggacgccgct ccaggcgagt accggttccg
     3601 gctgaggctt cgcacgccgg gaggccgtga gctcctttcg gtg
//
LOCUS       DAOSPL010000288         3637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25578_length_3637_cov_3.795645, whole genome shotgun sequence.
ACCESSION   DAOSPL010000288 DAOSPL010000000
VERSION     DAOSPL010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25578_length_3637_cov_3.795645"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2378)
                     /locus_tag="PLQ54_06625"
     CDS             complement(<1..2378)
                     /locus_tag="PLQ54_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92970.1"
                     /translation="MALLMLVPLLGPLVGAGLACEFSTGVGWTPQPSYLSNPAASVDT
                     RAENGAAVLRVAGPGRGMKFRLDLGGARVRPEDFLALRYRARGLAHGYALHAEPGDRL
                     LDVVDLCQDGQWHTLALDLHSLGVRKPISALLTEVQCLDEPAEIAFDWIRAAPDPPDD
                     ADVYPPLPSASAEYAVDLSRLAALDPKPSWLGSPAESFGCQWVGADGIHLWASGAGRG
                     MKFSGALPQPVDLSGYRYAAIRYRATGTAPWADYVLWLAGEASGEADQWAQPMSLNDV
                     WADGEWHTHVARLPAGFVATHLALQVSSAAERGDLWMGGIRFCATRPRLPVGEVLPFE
                     RGWRGLRLSSGHFVTLNLGPDARASADEVVGLLHVAEWLPGGPIQVNGIPFRIPSSQG
                     PLLATGEDGEPMAPVRVGTKGTEAYLLLISRLPALAYGEPGVEPGPMTRTDQPERFVV
                     SIEYVDGLVDEVFPLRVASNRFELLDGVSVYGVPALRGVPVRRMALRNRMDSARFWLA
                     AATLNQGPPQLEEPPTLALPAPPEPEREVPLPEPARISSRGPVWTLHAGELHLSLHIG
                     DGIRLVDVRNGYMSPRPLCSHPTPIFELGDGTRTLTSAQVSTEEPRVDTGPGGVVLTV
                     PFDARAAGIAATGQLIVAAAETGGICLRLTVQHMGSQSWTPQIEFPLIGPLTTGRADD
                     DWYLWCRKGGLVSNRSTHQRQSYGGAYPLQVADFFCPSGGGLALLTQDQTGSHRDWEL
                     SKDERGIQWRLSAWAREHAAGESVDIAPVLLWAHPGDWRAALRLYQRWAHKW"
     gene            complement(2432..>3637)
                     /locus_tag="PLQ54_06630"
     CDS             complement(2432..>3637)
                     /locus_tag="PLQ54_06630"
                     /inference="COORDINATES: protein motif:HMM:NF014948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HOS92971.1"
                     /translation="VDEQAAAAAVREAGLGVPVLIFGCQEEEVLRRSTPRRDAFCGLL
                     SIGDALRQIGVPYSIGQRPICFPTDDSFSRDLDWFVRVCRVVNGVSGGRYAQIGARPE
                     GFWTCRYSERELQAIGATTVVADLSEAIAGANAIASDDPQVRNTLDQIRTYADTSAIT
                     PEALDRMARFEVYLRRFREEHDIDAFAIQCWNSIQKNYGICTCSTMSRLGDDGIPCAC
                     EVDVLGAVSMHALQLASGTPAALADWNNLHNTDEELANIWHCGVFPKSFAQSHPTMGC
                     NDILCGSGACTRDQGQGVLSFRMRPGPLTLFRIGEDAGEPLKALVAQGVVEDNEAETF
                     GAYGWCRIPNLQRLYRDVLLNHSPHHVAITQGHVGNVLWEAFGKYLSLDVFHATQETP
                     GLYTPRLPF"
BASE COUNT          636 a         1194 c         1270 g          537 t
ORIGIN      
        1 taccacttgt gggcccagcg ctgatagagc ctcaacgcgg cccgccagtc ccccgggtga
       61 gcccagagaa gcaccggagc gatatcgacc gactcgcccg ccgcgtgttc gcgcgcccaa
      121 gccgacaggc gccattggat gccccgctcg tccttggaaa gctcccagtc gcgatgcgag
      181 ccggtctggt cctgcgtcag caacgccagg ccgccgcccg atggacagaa gaagtcagcg
      241 acctggagtg gataggcccc gccataggat tgacgctggt gtgtggatcg gttggacacg
      301 aggccgccct tacggcacca caggtaccag tcgtcatcgg cccgccccgt ggtaagcggg
      361 ccgatgagcg gaaactcgat ctgcggggtc caggactggc tgcccatgtg ctgaaccgtc
      421 agccgcaggc agatgccgcc cgtctccgcc gcggcgacga tgagctggcc agtcgcagca
      481 atgccggccg cgcgggcatc gaagggcacc gtgagcacca cccctcctgg gcccgtgtcg
      541 acccggggct cctcagtgct cacttgggcc gatgtcagcg tccgcgtgcc atcgccgagc
      601 tcgaagatcg gcgttgggtg cgaacagagg gggcgaggcg acatgtagcc attccgcaca
      661 tccaccaacc ggattccgtc accgatgtgg aggctcagat ggagttcacc tgcgtgcagc
      721 gtccacacgg ggccccgcga ggagatgcgg gcgggctcgg gcagaggcac ctcgcgttcg
      781 ggttcgggtg gcgccggcaa cgccagagtg ggtggctcct caagctgcgg cgggccctgg
      841 ttgagcgtgg ccgcggccaa ccagaatcgg gcggagtcca tccggttgcg cagcgccatc
      901 cgtcggacgg gtacaccccg gagcgccgga acaccgtaga cgctcacgcc gtcgagcagc
      961 tcgaaccgat tcgaggccac ccggagaggg aacacctcat cgacgagacc gtcgacatac
     1021 tctatgctca ccacaaatcg ctcgggctgg tcggttcggg tcatgggccc cggctcgacc
     1081 cccggctcgc cataggcgag agccggcagg cgcgagatca gcagtaggta ggcctccgtt
     1141 cccttggtgc ccacgcgtac cggggccatc ggctctccgt cctccccagt ggccaacagg
     1201 gggccctggc ttgaggggat acggaaagga atcccgttga cctggatggg tccgccgggg
     1261 agccactccg ccacgtggag cagtccgacg acctcgtcag ccgacgctcg ggcatctgga
     1321 cccaggttca gcgttacgaa gtgccccgac gacagccgga ggcccctcca tcccctttcg
     1381 aatgggagaa cctcgcccac gggcagacgc ggccgggtgg cgcagaagcg aatgcccccc
     1441 atccacaagt cgcctcgctc tgcggcggag ctgacctgaa gggcgaggtg cgtggccaca
     1501 aatccagccg gaaggcgtgc gacatgcgta tgccactcgc cgtccgccca gacgtcgttg
     1561 agcgacatgg gctgggccca ctggtcggct tcgcctgagg cctcgcccgc caaccacagc
     1621 acgtagtcgg cccagggcgc ggtcccggtg gcccggtagc ggatggcggc gtaccggtag
     1681 ccgctgaggt ccaccggctg gggcagagcg ccgctgaact tcatcccgcg ccccgcgccc
     1741 gacgcccaga ggtggatgcc atcggccccc acccactggc acccaaagga ctcagcgggc
     1801 gagccgagcc aggagggttt ggggtccagc gcggccaagc gagacaggtc tacggcgtac
     1861 tcggccgagg cggacgggag cgggggatag acgtcggcat cgtcgggtgg gtcgggcgcg
     1921 gctcggatcc agtcgaaggc gatctctgct ggttcgtcca agcactgaac ctcggtcagg
     1981 agtgccgaga tcggcttccg gacacccagg gagtggagat cgagagccag cgtgtgccac
     2041 tgcccatcct ggcacaggtc cacgacgtct agaagccggt cgcccggctc ggcgtggagg
     2101 gcatagccgt gggcgagacc tcgggcccgg tagcggagag ccaggaaatc ctccggacgg
     2161 acccgtgcac cgccgagatc gaggcggaac ttcatccccc ggccgggccc ggccacgcgg
     2221 agcacggccg cgccattctc ggctcgggtg tccactgagg cagcggggtt cgagaggtag
     2281 ctgggctggg gcgtccaacc cacaccggta ctgaactcgc acgcaagtcc cgctcccacg
     2341 agtgggccaa gcagcggaac gagcatcagc agagccattg aggactccct gcgtttcgga
     2401 gcgagggcgg cgggcccgat cgctcaccgg gtcagaaagg caagcgcggt gtgtacaggc
     2461 ccggggtctc ctgagtggca tggaagacgt ccagactcag gtacttgccg aaggcctccc
     2521 acagcacatt gccgacatgc ccttgggtga tggcgacatg gtggggactg tggttgagaa
     2581 ggacatcgcg gtagagccgc tgcagattgg ggatccggca ccatccatag gcgccgaagg
     2641 tctctgcctc gttgtcctcg accacgccct gagcaacgag agctttgagg ggctcgcccg
     2701 catcctcccc gatgcggaac agcgtgaggg ggcctggacg catccggaac gagagcacgc
     2761 cttggccctg atcccgggtg cacgcgccgg acccgcagag gatgtcgttg catcccatgg
     2821 tgggatgcga ctgggcgaag ctctttggga acacgccaca gtgccagatg ttggcgagct
     2881 cctcatcggt gttgtggagg ttgttccagt cggcgagcgc cgccggcgtt ccgctggcca
     2941 gctgcagggc gtgcatcgag accgcgccca gcacatcgac ctcacaagca cacgggatgc
     3001 cgtcgtcacc caggcggctc atggtggaac aggtgcatat gccgtagttc ttctggatgg
     3061 agttccagca ctggatggcg aaggcgtcga tgtcatgctc ctcgcggaag cgacggaggt
     3121 agacctcgaa tcgggccatg cggtccaggg cttcgggggt gatggccgac gtgtcggcat
     3181 aggtccggat ctggtcgagg gtgttgcgga cttgcgggtc gtcgctggca atggcgttgg
     3241 cgccggcgat ggcctcggag agatcggcga cgacggtagt cgcgccgatg gcctgaagtt
     3301 cgcgctcgga gtagcgacag gtccagaaac cctcgggccg cgcgccgatc tgcgcatagc
     3361 ggccgccact cacaccgttg acgacgcggc agacgcggac gaaccagtcg aggtcccggc
     3421 tgaaggagtc gtcggtgggg aagcagatgg gccgctggcc gatggagtaa gggaccccga
     3481 tctgccgcag ggcatcgcca atggaaagca ggccgcagaa cgcgtctcgg cgcggtgtgg
     3541 acctcctgag cacctcctcc tcctggcacc cgaagatcag aacgggcaca cccaacccgg
     3601 cctcacgcac ggcggcggcg gccgcctgct cgtcaac
//
LOCUS       DAOSPL010000289         3635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25599_length_3635_cov_3.867039, whole genome shotgun sequence.
ACCESSION   DAOSPL010000289 DAOSPL010000000
VERSION     DAOSPL010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25599_length_3635_cov_3.867039"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLQ54_06635"
     CDS             <1..405
                     /locus_tag="PLQ54_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92972.1"
                     /translation="LAEVRVGLLWCSEEPPARPDEGIALGITAGYNTQGLFTVRGGPA
                     FHIGDSFLVAGDLTWIQNQAALGYNVGAEGEIGDHVALRIGTHNSLGTTGAVLCLGAG
                     YKSDDDGWRVDVGWEYLNNATQGLMVSAGTTF"
     gene            868..2067
                     /locus_tag="PLQ54_06640"
     CDS             868..2067
                     /locus_tag="PLQ54_06640"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOS92973.1"
                     /translation="MSRYGRHKARYGLAMAVVLALLPLLAGCSVLEGIGNGNDEADGS
                     VDKDTNRGPFLFDVSPTTATIVGVAHVPLARQAGRAAEVRLDISPPATVADTSTATDG
                     SYWFELTGLTPATLYTYVVELGGEACSEAYQFRTAPLGSSSTRLAVFGDTRDNPRVLS
                     NLAALIRSWDPDLVIHVGDFVGDDEASEWQEQWYTPTQELTAQYWLLPVQGNHEEDSR
                     EYATWLGRAKLHSDTLLSGSVYSEVCGLVWIGVLNANHRMNEGSAQYAWLEGQLESAA
                     ARNATWRVVAVHEPPFHSSTKHQPGNEDVAQDLLPLLKQNEVQLLVTGHVHAYERLQD
                     DDLTIIITGAGGAPLHGDRISSPPPQSQVFVAEYEACLLDMTRTRLTASVQTPSGTQL
                     DQFVLTP"
     gene            2064..>2362
                     /locus_tag="PLQ54_06645"
     CDS             2064..>2362
                     /locus_tag="PLQ54_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92974.1"
                     /translation="MTGFVPAAVLGRHCGADAYDAGQEARGHGVLGFVPAAVLGRHCG
                     ADAYDAGQEARGHGVLGFVPAAVLGRHCGADAYDAGQEARGHGVLGFVPAAVLGR"
     assembly_gap    2363..2462
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2602..3006)
                     /locus_tag="PLQ54_06650"
     CDS             complement(2602..3006)
                     /locus_tag="PLQ54_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92975.1"
                     /translation="MRLTLGLAIVGVALLAGAVCAEPAWEVYLSKTQDHQAIDIKDPV
                     PVRNTVFVAMDDVAAAAGATIQSDEERNRVVVVKGDHTRSIRCNFTDLGARKINGNWY
                     CAHDVMAGLLFGKSYFSRGQSEINMVVDVEGD"
     gene            complement(3399..>3635)
                     /locus_tag="PLQ54_06655"
     CDS             complement(3399..>3635)
                     /locus_tag="PLQ54_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92976.1"
                     /translation="GDTLTLSVSFRADPRLCRVAAESSEPFAVAGAGPRPGGGLGLGL
                     FLAKQSAERLGGSLLVGADAERVEWNVTLPRRAS"
BASE COUNT          558 a         1228 c         1185 g          564 t
ORIGIN      
        1 ctggccgagg ttcgggttgg cttgttgtgg tgctcggagg agcccccggc acggcctgat
       61 gagggcatcg ctctgggcat tacggcgggg tacaacaccc aaggcctgtt tacggtccgt
      121 ggcggcccgg catttcacat cggtgacagc ttcctggtgg cgggcgacct cacgtggatc
      181 cagaatcagg ccgccctcgg ctacaacgtg ggcgccgagg gtgagatcgg cgaccacgtg
      241 gccctgcgca ttggcaccca caactcgctg ggcaccaccg gcgcagtgct ctgcctcggt
      301 gcgggctaca agagcgatga cgacggctgg cgtgtcgatg tcggctggga gtatctcaac
      361 aacgcaaccc agggtctgat ggtgagcgcg gggacgacct tctagcacga cccactcggc
      421 acactgacct tcgcgggggc gctccggttc ggagggcccc cgttttgcat ctgggggcgg
      481 catggcgcga cgttgggcgc agcccgctgc cagtgcgagg tcggcgagtt cgaggaggga
      541 ctcgaggcgt tccgcaaggc cgatgccatc gccccgcgcc tccgcccatg gccgatcgag
      601 ctggcgtagt tgctcctgct gttggggatg cgcgacgagg ctcgagggga gctcgaggac
      661 ctggaccgat tggacccgcc gagtcccggc cccacgggcg gcctgcggtc tcgcatggcc
      721 tgaggtatcg cgctcgcgtc acgccgcgcc ggtgcttgtg tgcccgcccc gggttagggg
      781 tctgcgcgcc cggggtaacc tctgtgtgtg ccggggccgc gatgccgggc cgggcgtacc
      841 ccactccaag gacaggggca cactcacatg agcagatacg gacggcacaa ggcccgttac
      901 gggttggcca tggctgtggt gctggcactg ctgccattgc tggccggctg cagcgtgctc
      961 gaggggattg ggaacggcaa cgacgaggcc gatggctcgg tcgacaagga caccaaccgc
     1021 gggcccttct tgttcgatgt gtcgccgacg actgccacca tcgtcggagt ggcccacgtc
     1081 ccactcgcgc gtcaggcggg ccgggcggcc gaggtcaggc tcgacatctc cccgccggcc
     1141 acagtcgccg acaccagcac ggccaccgac ggctcctact ggttcgagct cacaggtctc
     1201 accccggcga cactgtatac ctacgtggtc gaactcggcg gcgaggcctg ctccgaagcc
     1261 taccagttcc gtaccgcacc cctggggagt tcgagtaccc gtttggccgt gttcggcgac
     1321 acgcgcgaca acccgcgcgt cctctcgaac ctggcggcgc tgatccggtc gtgggatccc
     1381 gacctggtca tccatgtggg cgacttcgtg ggcgacgacg aggccagcga gtggcaggag
     1441 cagtggtaca cacccacgca ggaactcacc gcccagtact ggctcctccc cgtccagggc
     1501 aaccatgagg aggacagccg cgagtacgcc acttggctcg gccgcgccaa gctccacagt
     1561 gacacgctcc tcagtgggag tgtctattct gaggtctgcg gcctggtctg gatcggcgtc
     1621 ctgaacgcca accatcggat gaacgagggt tcggcccagt acgcctggct ggaaggccaa
     1681 cttgagtccg cagccgcccg caacgccaca tggcgcgtgg tggcggtcca cgaaccgcct
     1741 ttccactcgt ccaccaagca ccagccgggc aacgaggatg tggctcagga tctgctcccg
     1801 ctcctgaagc agaacgaggt gcagctcctg gtgaccgggc atgtccacgc ctacgagcgc
     1861 ctgcaggacg acgacctgac gatcatcatc acgggggcgg gaggtgcgcc gcttcatggc
     1921 gaccggatca gctcgccgcc gccacagagc caggtcttcg tcgccgagta tgaggcctgc
     1981 ctgttggaca tgacccggac gaggctgacg gccagcgtcc agacgcccag cggtacacag
     2041 ctcgaccagt tcgtgctcac gccgtgacgg gtttcgtgcc ggcggccgtc ctcggccgcc
     2101 actgcggggc cgacgcctac gatgcgggcc aggaggcccg cgggcacgga gtactgggtt
     2161 tcgtgccggc ggccgtcctc ggccgccact gtggggccga cgcctacgat gcgggccagg
     2221 aggcccgcgg ccacggggta ctgggtttcg tgccggcggc cgtcctcggc cgccactgcg
     2281 gggccgacgc ctacgatgcg ggccaggagg cccgcgggca cggagtactg ggtttcgtgc
     2341 cggcggccgt cctcggccgc cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnaggcccgc gggcacggag tactgggttt cgtgccggcg gccgtcctcg gccgccagtt
     2521 tcctacccca ccaaaggctg cgggctcacc cgaaggtgag cccgcaatcc cccgacgatt
     2581 agtccggact aatcgcctcg actagtcacc ctccacgtcc acgaccatgt tgatctcgct
     2641 ctggccgcgg ctgaagtagg acttgccgaa cagcagccca gccatgacgt cgtgagcgca
     2701 gtaccagttg ccgttgatct tgcgggcgcc caggtcggtg aagttgcagc ggatcgaccg
     2761 ggtgtgatcg cccttgacca cgaccacgcg gttgcgctcc tcgtcactct ggatggtcgc
     2821 gccagcggcg gcggcgacgt catccatcgc cacgaacacg gtgttgcgca ccggaaccgg
     2881 atccttgatg tcaatcgcct ggtggtcctg agtcttgctc aggtacacct cccaggccgg
     2941 ctcggcacac accgcacccg ccagaagggc tacgcccaca atcgcgagtc cgagggtcag
     3001 cctcatgctc tcgattcacc tcctctgcca catgccgtca gttgccaggg caccgagcta
     3061 catcggaggc cggtggaccg ttggtcccag cgcatccgag cctcgtccct cagctcctat
     3121 ggccagcccc ggcgtgccag ctgcggtgcg gcaggctcag gttggccttc cggcaggttg
     3181 ttccggcggc gacaggcttt caccttctcc gggcgcaaaa gacccacccc agggcagacc
     3241 cggcacccca agtgcaggcg ccgccgcccg gcccgccggt ggagttctgc cccaaccggt
     3301 ctaaatccac caacaggatg ggcggttcat tgcgtcccaa gtcctccgcg gacccatccc
     3361 accgagcgag ccgggtatac gtccgggcag tctctttgtc aagatgcacg ccgcgggagg
     3421 gtcacattcc attcgacacg ctcggcgtcc gccccgacca ggagagatcc gcccagccgc
     3481 tccgccgact gcttggcaag gaacaatccc aggcccagcc ccccaccggg gcgagggccc
     3541 gctccggcca cggcaaaggg ttcggaggac tcggcggcca cccggcacag cctggggtcg
     3601 gcgcggaacg agacggagag ggtgagcgtg tcacc
//
LOCUS       DAOSPL010000290         3626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25682_length_3626_cov_3.545785, whole genome shotgun sequence.
ACCESSION   DAOSPL010000290 DAOSPL010000000
VERSION     DAOSPL010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25682_length_3626_cov_3.545785"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PLQ54_06660"
     CDS             complement(<1..798)
                     /locus_tag="PLQ54_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOS92977.1"
                     /translation="MTAYRVTFSIRGMDCAACARHVENRLRAIDGVSDATVDAVTEHC
                     IVLCDGAAVSEADLYAAVRELGYHPAATLEPRRHPIRRQEQRLLLRIGLAAVLLPLAW
                     KGPEATSTPLAIAATIVTGYSILWQAGRSVRRRAMDVETFLSLGIVACCAVGEFLAAA
                     TIGVFVLVAQVLEIHTSDRSRRGIRELVAATPHSALVRRGGDVARIPADQLTCGDTVL
                     VRPGTRIPADGHVLSGQAAVNEAPITGESTPKEKGPGDRVFAGSITDA"
     gene            993..2132
                     /locus_tag="PLQ54_06665"
     CDS             993..2132
                     /locus_tag="PLQ54_06665"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS92978.1"
                     /translation="MDRVRMGIIGTGGMGSAHVAIARELDCVTLAAVCDIDEAVCRAK
                     SEECGVPGFTNAEELLDSGLVDAVIIATPHYFHPPIAIAAFRRGIHVLSEKPIAVTVS
                     AADAMIAAAKASGCKFAVMYQMRTESIHIAARRIIEEGGLGDIYRTSMIMGWYRSQAY
                     YDSGGWRATWTGEGGGVLINQAPHMLDIFTWLGGLPTMIHGRTRTRLHEIEVEDEAFA
                     VLEYANGAHGYLYASTTEAPGTDVLEIAGDRGKIRIQEGRLRYWRLEEARSEHAAGAT
                     EMWSSPKSEEVEVPLPEVQSGHKAVQANFVDAILHDTPLVTPGEEGINALEMINGMIL
                     SGHKGQPVTVPVDRAEYDALIESLKASSSGKARVREQRVTDPKFQ"
     gene            2143..3540
                     /gene="mnmE"
                     /locus_tag="PLQ54_06670"
     CDS             2143..3540
                     /gene="mnmE"
                     /locus_tag="PLQ54_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOS92979.1"
                     /translation="MGDHGLNPDTIVQRATPAGEGGLAVVRVSGPEARSMAEAVFRRA
                     TAPARPLRWRHAYYGRLVDPASGRDVDEVVLTLYAPGASFTGEAMAEIGCHAGPIAVG
                     RVIELFQRLGARPAEPGEFSKRAFLNGRLDLCQAEAICDLVRARSDEAAQAALRQLDG
                     GLSERVRGLRDSLMGLLAEIEVTIDFPDDDLVPVTTDHAERRCEEAMRALDDLLSRAE
                     VGRSLREGVVVAIAGRPNVGKSSLLNGLLGRQRAIVTSEPGTTRDLIEEQITLAGIPF
                     RLVDTAGLRAASGEVEAMGVDLALRAIETADICLVVLDASMPIEAEDRRVMDQVRERC
                     ARRVGVLNKRDLPARLTAEQASAALACSAWVHTSAIEPGGTTELERALAELVAGGGIS
                     AEGELLTNARHAHLMRMARAELDSAAAAAREGVPVDLVSIRIRAAAEHLGEITGETLV
                     PELVDRIFSTFCLGK"
BASE COUNT          577 a         1141 c         1335 g          573 t
ORIGIN      
        1 tgcatccgtg atactaccgg cgaagacgcg gtcacccggc cccttctcct tgggtgtgga
       61 ttcgccggtg atgggtgctt cgttgacggc cgcctgcccg ctgagcacgt ggccgtcggc
      121 cgggatgcgg gtgcccgggc gtacgagcac cgtgtcgccg caggtgagtt ggtcggccgg
      181 gatgcgggcc acgtcgcccc cgcggcgcac gagggccgag tggggtgtgg cggccacgag
      241 ctctcggatc ccgcggcggc tccggtcgga tgtgtggatc tccagtactt gggcaacgag
      301 cacgaagaca ccgatggtgg cggcggccag gaactcgccc acggcgcagc atgcgacaat
      361 gcccagcgac aggaacgtct cgacatccat ggcccggcgg cggacggagc gcccggcctg
      421 ccagaggatc gagtagccgg tgacgatggt ggccgcgatc gcgagcgggg tcgaggtggc
      481 ctccggcccc ttccaggcga gggggagcag gacggcagcg aggccgatcc gcagaagaag
      541 cctctgctct tggcggcgga tgggatggcg gcggggctcg agggtggctg ccgggtggta
      601 gccgagttcg cggacggccg cgtacaggtc agcctccgag acggcggctc catcgcacag
      661 cacgatgcaa tgctcggtca ccgcgtcaac ggtggcatca ctcacgccgt caatggcgcg
      721 cagacggttc tcgacgtgcc gcgcgcacgc cgcgcagtcc atgccccgga tgctgaaagt
      781 gacgcgatag gcagtcacgg gttactccgt ccggcctcca gtgccatcgg cgaggcccat
      841 ggggatcaac gccatcgacc tggcggcagt tccccgcgtt cattgtgccg catggcggca
      901 cgcaggagac ccggtttggc cctgggaaga tccggacgaa cacaccccgc ggctgccccc
      961 gagggggcgc gctcggcctg gaggagcaca tcgtggacag ggtacgtatg ggcatcattg
     1021 gtaccggcgg gatgggcagc gcccacgtcg ccattgcgag ggagctggac tgcgtcacac
     1081 tggcggccgt gtgcgatatc gacgaggccg tatgccgcgc caagtcggag gagtgtggcg
     1141 ttccgggctt cacgaacgct gaggagttac tcgacagcgg tctggtggac gcggtcatca
     1201 tcgccacgcc gcactatttc catccgccca ttgccattgc ggcttttcgc cgggggatcc
     1261 atgtgctgtc cgagaaaccc atcgcggtca ccgtttcggc cgcggacgcg atgatcgcag
     1321 ccgccaaggc atcgggctgt aagttcgccg ttatgtacca gatgcgtacg gagtccatcc
     1381 acatcgcggc gcgccggatt attgaggaag gcggactcgg cgacatctac cgcacatcga
     1441 tgatcatggg ctggtaccgg agccaggcct actacgactc ggggggctgg cgggccacat
     1501 ggaccggtga gggcggtggc gtgctgatca accaggcgcc gcatatgctc gatatcttca
     1561 catggctggg tggcctcccg acgatgatcc acggccggac gcgcacgcgc ctgcacgaga
     1621 tcgaggtgga ggacgaggcc ttcgcggtcc tcgagtatgc caacggcgct cacgggtacc
     1681 tctatgccag cactactgaa gcgccgggga ccgatgtgct cgagatcgcc ggggaccggg
     1741 gcaagatccg gattcaggaa ggccggctgc gctactggcg tctggaggag gcgcggagcg
     1801 agcatgccgc cggcgcgacc gagatgtgga gctcgcccaa gagcgaggag gttgaggtcc
     1861 cgttgcccga ggtgcagtcg ggccacaagg cggtgcaggc caacttcgtc gacgccattc
     1921 ttcatgacac accgctggtg acgccaggcg aggaggggat caacgcactg gagatgatca
     1981 atggcatgat cctctcgggc cacaagggcc agccggtgac tgtgccggtt gaccgcgccg
     2041 agtacgacgc cctgatcgag tcgctcaagg cgtcgtcgag cgggaaggcg cgggtccgcg
     2101 agcagcgggt cacggatccg aagttccagt agccgcgcac gcatgggaga ccacggcctg
     2161 aaccccgaca cgatcgtcca gcgggccacg ccagccggcg agggtggcct ggcggtcgtg
     2221 cgcgtgagcg gcccagaggc ccggagcatg gccgaggccg tgttccgccg ggccacagcg
     2281 ccagcccggc cactccgctg gcggcatgcg tactatggac gcctcgtcga tccggcctcg
     2341 ggccgggatg tggacgaggt cgttctgacg ctgtatgccc ccggggccag cttcacgggc
     2401 gaggccatgg cggagatcgg gtgtcacgcc gggccgatcg cggtgggccg cgtgatcgag
     2461 ctgttccagc ggctcggggc gcgcccggcc gaaccgggcg agttctcgaa acgggccttt
     2521 ctcaatggcc ggctcgatct gtgccaggcc gaggccatat gcgacctggt ccgcgcccgg
     2581 agcgacgagg cggcccaggc tgcccttcgg cagctcgatg gcggcctgtc ggaacgcgtg
     2641 cgtggcctgc gcgactcgct gatgggtcta ctggcggaga tcgaggtcac catcgacttc
     2701 cccgatgacg atctggtgcc cgtgaccacg gatcatgccg aacggcggtg cgaggaggcc
     2761 atgcgggcgc tcgatgactt gctgtcacga gcggaggtgg ggcggtcgct gagggagggt
     2821 gtggtggttg ccatcgccgg gcggcccaat gtgggcaagt ccagcttgct caatgggctg
     2881 cttgggcgcc agagggccat cgtaacgtcc gagccgggga cgacgcggga tctgatcgag
     2941 gagcagatta cgcttgccgg tatccccttc cggcttgtgg ataccgccgg cctgcgggcg
     3001 gcgtccggtg aggtcgaggc catgggggtc gatctcgcgt tgagggccat cgagacggcg
     3061 gatatatgcc tcgtggtgct cgatgcgtcg atgcctatcg aggccgagga ccggcgagtg
     3121 atggaccagg tacgcgagcg ctgcgcccgg cgcgttggtg tgctcaacaa gcgtgacctc
     3181 ccggcgagac tgaccgcgga acaggcgtcg gccgcactgg cgtgtagcgc ctgggtgcac
     3241 acgtccgcca tcgagccggg cggtaccaca gaactcgaga gggccctggc tgagctcgtg
     3301 gcgggcgggg ggatctcggc tgagggcgag ttgcttacca acgctcggca cgcacacctg
     3361 atgcgcatgg cccgggccga gctggattcg gcagcggccg cagcgcgcga gggcgtgccc
     3421 gtcgacctcg tgtcgattcg gattcgcgct gcggccgagc acctgggcga gatcacgggc
     3481 gagaccctcg tgcccgagct tgtcgaccgg atcttctcga cgttctgcct ggggaaatag
     3541 ggccgtcagc gttcggtatc cccgggtgcg gctacgcctc cgcatccctc atccaggcca
     3601 ggaactcttc accgaaccac cgctcg
//
LOCUS       DAOSPL010000291         3624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25707_length_3624_cov_3.317176, whole genome shotgun sequence.
ACCESSION   DAOSPL010000291 DAOSPL010000000
VERSION     DAOSPL010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25707_length_3624_cov_3.317176"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLQ54_06675"
     CDS             <1..310
                     /locus_tag="PLQ54_06675"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOS92980.1"
                     /translation="RRVAGAGFRVDTLPATIARDGSATVSFRLSETAQVDCVIKNAAG
                     RIVRRVAVDKTLEGGTRTLVWDGRTDTGSVCPRGMYTVEIVARNDRMERAHAVQALSR
                     "
     gene            350..3238
                     /locus_tag="PLQ54_06680"
     CDS             350..3238
                     /locus_tag="PLQ54_06680"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOS92981.1"
                     /translation="MTDGLRLRGVLALATLGALGVVVGAWADSFPLIQPYTIQNDFVR
                     IRVGDILAGDEISSTENYPGHVRQKARWVMDTTGGEPTVTGDENQDLTSPRWIADGSD
                     IIQGINGYTRIAIGDPSEAEPEYTYAIYGAVGDGDDEGTFVSGGGVIQNDLIETYWRP
                     NAQNYVEITQALQIVRDKVRVEYIVHNQSTTQYALIGLRPLIDCHRGATDSGPIYFAQ
                     HAENGQAVGGKEESFRVEREWGTKTNPLRRWFWFFAPDSVDSPETVCGGIVESPPEPS
                     SGSKDASVPPDRVLLANWARVFGTEWDATADDSRFAGDDLCLLIYWSPEDRNGRLRTI
                     PPGGKRRYVFYYGLSSATEDVTAKSVAGRPALVGATMAPFALHLTADQQAYDLPSPNG
                     FPIRVDVTTGPGTTSLTGNAAALSLPDGLTTTDARSQSIANLGPYATGSAQWIVRPTG
                     ERYGTFTYTVTVTSQGGGSKSIVRTIEVPALPTLTTADVAPLDPPYAGEVPGACYRMF
                     SVPYGLDDSDSLYGLNYVAKANAARQVTPRIFAYDPATRRYDEYPEGAKAREITPGRG
                     YWLLVDEAVQIQVQAGNARPLNAATYVAVPLEAAGGGFNQVGSPFTYAVRLRDVSFQF
                     GEKQLTYEEAVAAGWVRGTVWYWDPTGRSGRGSYGYFGTPDNTLDPWVGYWIKALVDC
                     RMVLTPPTSLGALAPTSALGVRSSAGSALKARSGGTREDWLMQLTATTAEAEDSANFI
                     GVAPSARTGYDPSDVEEPPVIGPYVQLSFPHTNWGRDSGLFTQDIRSAKSGTVSWDAQ
                     VRTNLVGQDVTIAWPTIGKLPRDLEAYLEDLDTGKRVFMRSSAGYTFRAGESGVRNFR
                     VVVTTAGQGLVISGMEVEQLARGAGAQIAFALSAPAHVDAVVYNAAGRQVAQVLSDYA
                     GVAGRNTISWNGRGTSGTALPAGQYRVQVTARSVDGGQTTATRMLSIR"
     gene            3427..>3624
                     /locus_tag="PLQ54_06685"
     CDS             3427..>3624
                     /locus_tag="PLQ54_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92982.1"
                     /translation="MGNRSRPARRWATGRGISACLALGVFISHGAGRALGQEVQRIEL
                     QCARTEIIADGRDSAIIEALVT"
BASE COUNT          628 a         1200 c         1226 g          570 t
ORIGIN      
        1 gcgccgcgtg gccggggccg gcttccgggt ggacactttg cccgcgacca tcgcccgcga
       61 tggcagcgcc acggtgtcat tccggctcag cgagacggcg caggtcgact gtgtcatcaa
      121 gaacgcggca ggacgcatcg tgcgccgtgt ggcagtcgac aagacgctcg aaggcggcac
      181 gcgcactctg gtgtgggacg gccgcacgga cacgggcagt gtctgccccc ggggtatgta
      241 cacggtcgag atcgtggccc ggaacgatcg catggagcgg gctcacgcgg ttcaggcatt
      301 gtcgcgatag ccgacgtggc tgcttgcgat gggtacggag gggtatgtca tgacggatgg
      361 gctccggctt cgcggagtgc tcgcgctggc aacgcttggg gcactagggg tcgttgtggg
      421 cgcgtgggcg gactccttcc cactgatcca gccgtatacg atccagaacg acttcgtgag
      481 gatccgggtg ggcgatattc tggccggcga tgagatctcg tcgaccgaga actaccccgg
      541 tcacgttcgc cagaaggctc ggtgggtgat ggacaccacg ggcggcgaac ccacggtgac
      601 cggcgacgag aatcaagacc tcacatcgcc ccgctggatc gccgatggca gtgacatcat
      661 ccagggcatc aacgggtaca cccgcatcgc catcggggat ccgtcggagg ccgagcccga
      721 gtacacctat gccatctatg gcgccgtggg ggatggcgac gacgagggca ccttcgtctc
      781 gggcggcggg gtcatccaga acgacctgat cgagacctac tggcgaccca acgcgcagaa
      841 ctacgtagag atcacgcagg ccctgcagat cgttcgagac aaggtgcgcg tcgagtacat
      901 cgttcacaac cagagcacca cacagtatgc cctcatcgga ctccgtcccc tgatcgattg
      961 ccaccgcggc gccaccgact cgggccccat ctacttcgcc cagcatgccg agaacggcca
     1021 agccgtcggc ggcaaggaag agagcttccg cgtcgaacgc gagtggggga cgaagacgaa
     1081 ccctttgcgc cgctggttct ggttcttcgc cccggactct gtcgactctc cggagaccgt
     1141 gtgtgggggc atcgtggaaa gccctccgga gccatcgagt gggagcaagg atgcctcagt
     1201 tccccccgac cgggtgctct tggccaactg ggcccgggtg ttcggcaccg agtgggatgc
     1261 caccgccgac gacagccggt tcgcgggcga cgatctgtgc ttgctgatct actggtcgcc
     1321 ggaggaccgt aacggccggc tccgcacgat tccccctggg ggaaagcgcc gctacgtctt
     1381 ctactatggc ctctccagcg ccaccgagga tgtcacggcc aagagtgttg ccggtcggcc
     1441 cgcgctggtt ggcgcgacca tggccccctt tgcactgcac ctgacagccg atcagcaggc
     1501 gtacgacctc ccctcgccca atggctttcc cattcgcgtc gacgtgacga cgggtccggg
     1561 gacaacctcc ctgaccggca atgccgctgc cctgtcgctc ccagacggtc tcaccaccac
     1621 cgacgcccga agccagtcca tcgcgaacct tgggccatac gcaacggggt cagcccagtg
     1681 gatcgtgcgc cccaccggcg agcgctacgg gacgttcaca tacacagtga ccgtcacatc
     1741 ccaggggggt gggtcgaaga gcatcgtccg caccatcgag gtacccgcgc tgccgacact
     1801 aaccactgcc gacgtcgcgc ccctggaccc gccctacgcg ggagaggttc ccggagcctg
     1861 ctaccgcatg ttctcggtac cctatggact cgatgactcc gactcgctct atggcctcaa
     1921 ctacgttgcc aaggccaacg cggcccggca agtgacgcca cgcatcttcg cctatgaccc
     1981 cgccacccgg cggtatgatg agtatccgga gggagccaag gcccgggaga tcacgccggg
     2041 tcgcgggtac tggctgctgg tggacgaggc ggttcagatc caggtgcagg ccggtaatgc
     2101 acgccctctg aatgcggcca cctacgtcgc cgtgccgctg gaagcggccg ggggcgggtt
     2161 caaccaggta ggatcaccct tcacctacgc ggttcgcctg cgcgacgtga gcttccagtt
     2221 tggcgagaag caactgactt acgaggaagc cgtcgctgcc ggatgggtcc gcggtacggt
     2281 ctggtactgg gatcccaccg ggcgcagcgg ccgggggagc tatggctact tcggcacccc
     2341 ggacaacaca ctagacccct gggtcggcta ctggatcaag gccctggtgg actgccgaat
     2401 ggtcctgaca ccaccaacct cccttggggc actggctccc acgagtgcac tcggcgtccg
     2461 cagcagcgcg gggagcgccc tgaaagctcg gtcgggcggg acccgcgagg actggctgat
     2521 gcagctgacg gcaaccacgg ccgaggctga agactccgct aacttcatcg gtgtggcgcc
     2581 ctcggccaga acaggctacg acccatcgga cgtcgaggaa cctccggtga tcgggccgta
     2641 cgtacagctg agcttccccc acaccaactg gggccgtgac tcggggctgt tcacgcagga
     2701 catccgcagc gccaagagcg gcaccgtgtc gtgggacgcc caggtgcgga ccaatctggt
     2761 cgggcaggat gtgacgattg cctggccgac catcggcaaa ctaccgcggg acctcgaggc
     2821 ctacctggag gatctggaca cggggaagcg cgtgttcatg cgctcgtcgg ccggctacac
     2881 gttccgggcc ggcgagagcg gcgtccggaa cttccgcgtc gtcgtgacga ctgcaggcca
     2941 gggactggtg attagcggca tggaggttga gcagctcgcg cgaggcgccg gtgcgcagat
     3001 tgccttcgcg ctctcggccc ccgcccatgt cgatgcggtg gtctacaacg ccgcggggcg
     3061 tcaggtggcc caggtgctct cggactacgc cggtgtggcc ggtcggaaca ccatctcgtg
     3121 gaacgggcgg ggaacctcgg gcaccgctct gccggccggc cagtaccgcg tgcaggtcac
     3181 cgcccggtcg gtggacgggg gacagacgac cgccacccgc atgttgtcga tccgctagag
     3241 cacgcggcac agccaagaga cggcttcagg ccgccccccc tgggggcggc ctgaactgct
     3301 tgtaccggag cacggttggt tcactgaccg tccgagccgt gcaggagcgg cgcgaagggc
     3361 ggccgaagtg aagcgccatg tggggccctg acgccgccag cgctggggcc cgccctggac
     3421 ggtgtgatgg ggaaccggag tcgaccagcg cggcggtggg cgacggggcg aggcatctct
     3481 gcatgcctgg ccctgggcgt gttcatatcg cacggagccg gccgcgccct gggacaagag
     3541 gtacagcgca tcgagctgca gtgcgcccga acggagatca tcgccgacgg ccgcgactcg
     3601 gcaatcatcg aagccctggt cacc
//
LOCUS       DAOSPL010000292         3621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25744_length_3621_cov_3.092541, whole genome shotgun sequence.
ACCESSION   DAOSPL010000292 DAOSPL010000000
VERSION     DAOSPL010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25744_length_3621_cov_3.092541"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PLQ54_06690"
     CDS             complement(<1..664)
                     /locus_tag="PLQ54_06690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS92983.1"
                     /translation="MRIGHVTFSYHPITGGGDTYLAELLKVLEADGHDQRVYQRPVPG
                     PPPDGVRYIPDPLGCRLRRGQFWTVPLGLRRLRGELASEDILIAHYPNYHWAIEWHPR
                     TVLISHGVFWDDRPGALRSQIKRRLALRAYRSAGQVVANDTFYLREMGEDVPIGADPF
                     VEVAPGRWFIPNCVDTERFRPVMPMEGLSRLAPVLVPRNLFRNRGVHLAIQAFALLAP
                     DFP"
     gene            complement(765..2738)
                     /locus_tag="PLQ54_06695"
     CDS             complement(765..2738)
                     /locus_tag="PLQ54_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOS92984.1"
                     /translation="MNWYARVTRKMHFDMHTPTAVEDVGVDFDAERFADELEAAGVEA
                     ICFFAKCAYGWSYYPTRVGRRHPHLVTDTFPPAVDACHRRGIRVLGYYHVSGCEWAGD
                     HPEWHGRNADGSSQGDGSIPYSLCPIGPASDELIIPQLVEIVTLQPADGLFLDDLIGW
                     KTCYCESCRAGFGRTMPAGPADEGWDDYLAWRRAAASAFFGRAAAAVHAARPDALFGV
                     NYAGSYRHPDFLPPGLDYLTADVPEPESSSLNASLLLRQHATQPLPCDAMNSRMVHWW
                     ADWTQKPVTAIRQEFATVLANGGTTFLGDICYHRTAMPDPAVLANAGRAFALAREVEP
                     WVHDAEPVPDIAILNSAASHYLSSRSPNADPVPVKGVHLALLEAGLHTHILTEATLAE
                     HLPAYRGLVVPEQAHISAQACNAIREFVRAGGGLVVVGDSPLADILGVERIGTFDDDR
                     AYLVCEAPDLSPRDEAACPPRLVHGRGIRVRPTTAEVLAPHVAPLGGGPPHSGPPPDR
                     PSGDPAITLNDYGGGRAALVALPVATDYFGRGHAALAPLMAGLVRRVCTPLVEVRTPA
                     PVEVSLLRRGSSLLVHLVNYTASRHPGRPATVDHITPVHEVVVRLRLAGEPHLVGGSD
                     EAWAAVQVEGGLDLVLPRLDLWACAEVSLAGAP"
     gene            3016..3513
                     /locus_tag="PLQ54_06700"
     CDS             3016..3513
                     /locus_tag="PLQ54_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92985.1"
                     /translation="MTSTMCSAQVVLPTVVLVTSKVFSPTTGTYAAGQLVTGATQHKA
                     VFNTSQANYLPCRLRLDWEQYPSWAQSDRVSNGHYQYTYTRNAAVTMYSSPNPILKSW
                     YIPPDSGPRSYAFVAATTARPDVCSVQYPPPPPAPPADPDKQYAMGEEVQGDSDVTQI
                     NTPAP"
BASE COUNT          626 a         1167 c         1288 g          540 t
ORIGIN      
        1 cggggaagtc cggcgccaga agcgcgaaag cctggatcgc caggtgaacg ccccgattgc
       61 ggaacaggtt gcggggcaca aggaccggag ccaggcggga cagcccctcc atcggcatca
      121 ccggccgaaa cctctcggtg tccacgcagt tggggatgaa ccaccggccg ggggccacct
      181 cgacaaaggg atccgcacca atgggcacat cctcgcccat ctcgcggagg tagaaggtgt
      241 cattggcaac gacttgcccc gcggaccggt aggcccgaag tgcgagacgg cgcttgatct
      301 ggctgcgcag cgccccgggc cggtcatccc agaacacgcc gtgcgagatg agcacggtac
      361 gcgggtgcca ctcgatggcc caatggtagt ttgggtagtg agcaataagg atgtcctcgg
      421 aggccaactc gccgcgcagg cggcggaggc ccaggggtac tgtccagaac tgcccgcggc
      481 ggagccggca gcccaggggg tcgggtatgt agcgcacgcc gtccggtggc gggccgggca
      541 cgggacgctg gtagacgcgc tggtcgtggc cgtcggcttc gagcaccttc agcaactcgg
      601 cgaggtaagt gtcacccccg ccggtgattg ggtggtagct gaatgtaacg tggccgatgc
      661 gcatggtacc ccattcgagg ccgacccggg ccccagatca gcggattccc cacaagccga
      721 ctcagtgttc ccggctggtg ttcgccgaac ctgcgccggc ccaactacgg ggccccggcc
      781 agactcacct cagcgcaggc ccacaggtcc agacgtggca gcaccaggtc cagcccgccc
      841 tcaacttgga cggccgccca agcctcatcg ctgccgccca ccaagtgcgg ctctcctgcg
      901 aggcgtaggc gtacgaccac ctcgtgcacg ggcgtgatgt ggtcgacggt ggccggccgg
      961 cccgggtggc gtgaagccgt atagttcacc agatggacca gcagagagga tccccggcgc
     1021 agcagcgaca cctcgacggg tgccggagtg cggacctcga ccaggggtgt gcagacccgg
     1081 cgaacgaggc ccgccatgag cggcgcgagc gccgcgtgcc ctcgaccgaa gtagtcagtg
     1141 gccacgggga gggcgaccag tgcagcccgg ccgcccccat agtcattgag ggtgatggcc
     1201 ggatcgccgc tgggtctgtc agggggtggg cccgagtgag gcggcccgcc ccccaacggc
     1261 gccacgtggg gtgccagcac ctcggcggtc gtgggccgca ctcggatgcc ccggccatgg
     1321 acgagccggg gagggcaagc ggcctcgtcg cgcggggaca ggtcgggcgc ttcacacacg
     1381 aggtaggcgc ggtcgtcgtc gaatgtcccg atgcgctcaa cgccgaggat atcggcgaga
     1441 ggcgagtcac cgacgacgac aagcccgccg ccggcgcgca cgaactcgcg tatggcattg
     1501 cacgcctgcg cggagatgtg ggcctgctcg ggcaccacca ggccccggta ggccggcaga
     1561 tgctctgcca gggtcgcctc ggtgaggatg tgggtgtgca agcccgcctc gagcagcgcc
     1621 agatggacgc ccttgacggg aactgggtcg gcgttcgggc tccgcgacga caaatagtga
     1681 gacgcggcgc tgttgaggat ggcgatgtcg gggaccggct cggcgtcgtg gacccacggc
     1741 tcgacctcgc gtgcgagggc gaatgcccgg cccgcattgg cgagcacggc gggatcgggc
     1801 atggcagtgc ggtgatagca gatgtcgccg aggaacgtcg tgccgccgtt ggcgagcacg
     1861 gtggcaaact cttgccggat ggccgtgacg ggcttctggg tccagtcggc ccaccagtgc
     1921 accatgcgcg agttcatggc atcgcacggc aatggctggg tcgcgtgctg gcggagcagg
     1981 aggctggcgt tgagtgagct gctttcgggc tcgggcacat cggccgtaag gtagtcgagg
     2041 cccgggggca ggaagtccgg gtgccggtac gaacccgcgt agttgacgcc gaacagggca
     2101 tcgggccgcg cggcatggac ggccgcggcc gcgcgcccga aaaacgctga cgccgcggca
     2161 cggcgccaag ccaggtagtc atcccacccc tcgtcggccg gcccagccgg catggtgcgg
     2221 ccgaagccgg cgcggcagga ctcgcagtag caggtcttcc agccgatgag gtcatcgagg
     2281 aacaggccgt cggcgggctg gagggtcaca atctcgacca actgagggat gatgagctcg
     2341 tcgctggccg ggccgatggg gcaaagtgag taggggatcg agccgtcgcc ctgcgaggag
     2401 ccgtcggcgt tgcggccatg ccactcgggg tgatcgccgg cccactcgca tccgctgacg
     2461 tggtagtagc cgagcacgcg gataccacgc cgatggcagg cgtcgaccgc gggcgggaag
     2521 gtatcggtaa ccaggtgggg gtgcctgcgg cccacccggg tggggtagta gctccagccg
     2581 taggcgcact tggcgaagaa gcagatggcc tcgacaccgg cggcctcgag ttcgtcggcg
     2641 aaccgctcgg cgtcgaagtc gactcccacg tcctccacgg cagtcggagt gtgcatgtcg
     2701 aagtgcatct tccgcgtgac gcgcgcgtac cagttcatgg cgtctctccc gtcccgggtg
     2761 gtctctcgcg gaagggttcg cggtgccgtg gcggatccct gggtgggggg gctgcgttca
     2821 tcggcttcgc accgacctat agactacttg accaacaggt cgtccaggcc gtatggttac
     2881 ctgcctgtgt tggtgtcata agctatggcg atcacgcgct gaagggatgg agttgaatga
     2941 ccaagagaga gagagagagc tagttccccg gtatcttggc ctgggaccct cgtcgctgcg
     3001 ctggctttgt tggtgatgac aagcaccatg tgctcggctc aggttgtcct acccacagtg
     3061 gtgctcgtca catcgaaggt cttctcgccg accacgggca cgtatgcggc aggccagctc
     3121 gtgaccggtg ctacgcaaca caaagccgtg ttcaacacct cgcaagcgaa ctacctgcca
     3181 tgcaggctcc ggctcgactg ggagcagtat ccctcctggg cgcagagcga ccgggtctcg
     3241 aatggccact accagtacac ctacacgcgg aatgcggccg tgacgatgta ctcctccccg
     3301 aacccgatcc tcaagtcctg gtacatccca ccggactcgg gtccccgatc ctatgcgttc
     3361 gtggccgcga cgaccgcaag gccagacgtc tgctcggtgc agtacccacc ccctccgccc
     3421 gcacctccgg cggaccccga caagcagtac gcgatgggcg aggaagtcca gggggattcg
     3481 gacgtgaccc agatcaacac cccagcgccg tagccgagca gcgataggct gccttgcgcg
     3541 ggcagcggca ggccagagac cgccactgcc cgcgcatcct agaggggagc gtgccagcat
     3601 gtggtctccg agcatcgtag a
//
LOCUS       DAOSPL010000293         3621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25745_length_3621_cov_2.658721, whole genome shotgun sequence.
ACCESSION   DAOSPL010000293 DAOSPL010000000
VERSION     DAOSPL010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25745_length_3621_cov_2.658721"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PLQ54_06705"
     CDS             <1..432
                     /locus_tag="PLQ54_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92986.1"
                     /translation="HHMMCMACWWSGGETIGPRPDDTIAELFGRLRRAPETPVTLIEG
                     CCEVCNCCDGFHPATTRCVHAGGLIRDYKKDLDVLQKLGLAPGATMPARDLVHLMFER
                     IPTTKDICAYGDGVVRSTEWSICGDPNGSPGYARSRERGML"
     gene            complement(479..1510)
                     /locus_tag="PLQ54_06710"
     CDS             complement(479..1510)
                     /locus_tag="PLQ54_06710"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS92987.1"
                     /translation="MTPRERVVASIRRGPTDALPWQFDMTSAIRAKLRHYYQTEDLLT
                     ATGDHLVWTGLGTPRGYVPPAAAPGLYRTEFGAVWRREARDTQIGDWGDLIDYPLKEP
                     SLAGYAFPDGSAPGRADHVPLARQRYPDHFLVVSGPSLFEAAWALCGFENYLAYVMAE
                     PAFVGELTGRLAEFHCAQIAQLAGTNVDAVRLGDDWGFQDRLMVRHETWRRLFRESYR
                     RVFEAVHRVCPFVMLHSCGNITDILPDLIDVGLDVVHPLQPEAMDVERCQREFGRDIT
                     FWGGLGCQSTLPLGTPADVRAEVRHRLRLFAGGGYILAPAGAVPTDTPVENVVAIIEE
                     AKGQLGDRA"
     gene            complement(1507..2658)
                     /locus_tag="PLQ54_06715"
     CDS             complement(1507..2658)
                     /locus_tag="PLQ54_06715"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOS92988.1"
                     /translation="MEFAIRCPNQVVFGWGMIDQLPKFTSGLGRRPLMVTGRSALRAS
                     GRLDDILGALSASGAVVALFDQVEAEPSLDTVLRGIETARVHEADVVVAIGGGSALDV
                     GKAIAAVAYADGELAEFHAGREVTGRSLPIVAAPTTSGTGAEVTPNSVLTDRVAGVKA
                     SIRGAGLIPRVAIVDPELTVSLPPKETAYTGLDAFTQALEAFISTGASPYSDALALEA
                     IRATGSWLREAVAAGRNREARTQMALGSLLAGLALASARLGLVHGLAHPVGARYALPH
                     GLVCGRFLPAVLAFNQAAAAPRYHRAAQALGLESGTALVPWVTTLCEDLGASMPLSTV
                     GASEADIDALVDPVLSAGSTAYNPRKPTAGDVRELLRQAIDGERPRGQA"
     gene            2783..3460
                     /locus_tag="PLQ54_06720"
     CDS             2783..3460
                     /locus_tag="PLQ54_06720"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HOS92989.1"
                     /translation="MPSVERLRRPALAALVTVSAFCVAAVLVSAAGVPGVPSRRLLLA
                     ASTGFVASALVAGGLGSAAGRLMTLGLVGCWFGDYLGAIRFEAGALAFLAAHVLFCAA
                     FAVAGLSGLRVRVAVPVWLAITGGIAVWLVPLVPPENRPLVIGYCVAITAMVVLACGL
                     DSWDRRQLALLGAVCFYVSDIFVARWKFVDGAQWNAFGCYPLYYGACFLLALSSGGRR
                     TGGPAGA"
BASE COUNT          542 a         1272 c         1248 g          559 t
ORIGIN      
        1 caccatatga tgtgcatggc ctgctggtgg tcgggtggcg agacgatcgg cccccggccc
       61 gacgacacca tcgccgagct gtttgggcgt ctccggcgcg cgccggagac acccgtcacc
      121 ctgatcgagg ggtgctgcga agtctgcaac tgctgcgatg gcttccaccc ggcgaccacg
      181 cgatgcgtgc acgctggtgg gctcatccgc gactacaaga aggacctcga cgtgctccag
      241 aagctcggct tggcgcccgg tgcgaccatg ccggctcgcg acctcgtgca cctgatgttc
      301 gagcggatac ccacgaccaa ggacatctgt gcttacggcg acggcgtcgt acggtcaacg
      361 gagtggagca tatgcggcga cccgaacggc agccccggct acgcccggag tcgtgagagg
      421 ggcatgctgt gagaggtcgg cggctgcctg tctctcctcg ccgccgagcg ccgatgggct
      481 atgccctatc ccccaactgc cccttggcct cctcgatgat cgcgaccacg ttctcaacgg
      541 gggtatcggt cggcaccgcg ccggccggag ccaggatgta cccgcccccg gcgaacagcc
      601 gcagtcgatg tcgcacctcg gcccgcacgt cggccggcgt cccgagtggg agggtgctct
      661 gacagcccaa cccgccccag aaggtgatat cgcggccgaa ctccctctgg cagcgctcga
      721 cgtccatggc ctcgggctgt agcggatgga cgacatcgag cccgacgtcg ataaggtcgg
      781 gcaggatatc ggtgatgttt ccgcacgagt gcagcatcac gaagggacac acacgatgga
      841 ccgcctcgaa cacgcgccga taggactcgc ggaacagccg ccgccaggtc tcgtgccgca
      901 ccatgaggcg gtcctggaaa ccccagtcgt cgccgagccg cactgcatcc acgttggtgc
      961 ccgcgagctg ggcgatctgc gcgcagtgga actcggccag ccgaccggtc aactcaccca
     1021 cgaacgcggg ctcggccatc acatacgcca gatagttctc gaacccgcac agcgcccagg
     1081 cggcctcgaa caggctgggg cccgagacga ccaggaagtg atcgggatac cgctggcgcg
     1141 ccaaaggcac gtgatcggcc cggcccggcg ctgagccatc ggggaaggcg tacccggcca
     1201 gagatggctc tttcaatggg tagtcaatca ggtcacccca gtcgccgatc tgtgtgtccc
     1261 gcgcctcccg ccgccagacg gcgccaaact cagtccggta gagccctggc gctgcggctg
     1321 gcggcacgta cccgcgaggc gtcccgagcc cggtccagac caggtgatcg ccggtggccg
     1381 tgagcaggtc ctcggtctgg tagtagtgtc gcagcttggc gcggatggcg ctggtcatgt
     1441 cgaactgcca cggcagcgcg tccgttggcc cgcgccggat cgacgccacc actcgctccc
     1501 gcggggtcat gcctgacctc gcggccgctc gccatcgatc gcctgccgca acagctcccg
     1561 cacatcgcct gctgtgggct tccgcgggtt gtaggccgta gagcccgccg acagcaccgg
     1621 gtccacgagc gcgtcgatgt ccgcctcaga ggcgccgacg gtcgagagag gcatcgaggc
     1681 gccgagatcc tcgcacagcg tggtcaccca gggcacgagc gccgtacccg actcgaggcc
     1741 cagagcctgg gccgcccgat ggtacctggg cgccgccgcc gcctggttga acgccagcac
     1801 ggcgggcaag aaccgaccgc acaccaggcc atgcgggagg gcgtaacgag cccccaccgg
     1861 gtgggccaga ccatggacca gccccaggcg cgcgctcgcc agcgccagtc cggccagcag
     1921 gctgcccaga gccatctgcg tccgcgcctc gcggttccgt ccagcggcca cagcctcacg
     1981 aagccagctg ccagtcgccc ggatggcctc cagcgccagg gcgtcgctgt aggggctggc
     2041 accggtcgag atgaaggctt ccagcgcctg ggtgaaagca tcgagccccg tgtaggccgt
     2101 ctccttcgga gggagcgaga ccgtgagttc ggggtcgaca atggccacgc gcgggatcag
     2161 cccggcgccc cggatactcg ccttgacacc agccacccgg tcggtcaaca ccgagttcgg
     2221 cgtgacctcg gctccggtgc ccgaggtcgt cggcgccgcg acaatgggca gggatcggcc
     2281 ggtgacctct ctcccggcgt ggaactcggc cagctcgccg tcggcatagg ccacggcggc
     2341 gatggctttc cccacatcca gcgcgctccc gccaccgatg gcaacgacca cgtcggcctc
     2401 gtgaactcgc gccgtctcaa tgcctcgcag cacggtatcg agggacggtt cggcctcgac
     2461 ctggtcgaac agcgccacga ccgcaccgga ggcggacagt gcaccgagaa tgtcatccag
     2521 gcgccccgac gctcgaagcg ccgagcggcc ggtgaccatc agcggacgtc gtcccaggcc
     2581 cgatgtgaac ttcggcagct ggtcgatcat gccccatccg aagacgacct ggttcgggca
     2641 gcgaatggca aactccaaca gcgggcacct ccccgaggag ttcggaggtt cctctggctt
     2701 cgggacggcg agagtgagac ccttcccgcc ccgcgccccg ctatactgtg gccggccacg
     2761 ctgctgcaga agggaggctg cggtgccatc ggttgagaga cttcgccggc ctgctttggc
     2821 ggcactggtc accgtatcgg cgttctgtgt ggcggccgtc ctcgtctcgg cggccggggt
     2881 gccgggcgtt ccctcccgcc gtctgctctt ggccgcctcg acgggattcg tcgcctcggc
     2941 cctggtcgcc gggggactcg ggtccgcagc tgggcggctg atgaccctcg ggctggtggg
     3001 ctgctggttc ggcgactacc tcggggccat ccggttcgag gccggcgccc tggccttcct
     3061 ggcggcgcac gtgctgttct gtgcggcgtt cgctgtcgcg ggcctctccg gactgcgcgt
     3121 tcgcgtcgcc gtgccggtgt ggctcgccat cacaggcggc atcgcggtgt ggctcgtgcc
     3181 cctggttccg ccagagaacc gtccgctcgt catcggctac tgcgtcgcga tcacggcgat
     3241 ggtggtgctc gcctgcgggc tggactcgtg ggaccggcgg caactcgcac tgttgggcgc
     3301 ggtgtgcttc tatgtctcgg acatcttcgt ggcgcgctgg aagttcgtcg acggggccca
     3361 gtggaacgcc ttcggctgct acccgctgta ctatggggcc tgcttcttgc tggcgctgag
     3421 cagcggcggg aggcggacgg gcggcccagc gggcgcgtga gccgcggcgc ccggaacgct
     3481 ccgggtcgcc gccccctccc gggagctcaa gcgagctggt ggtcagcccc aaaaggggcg
     3541 atgtctgtgg tagcccgggg cgagagccct ggacggcgcg tgcggcccat ggtgcgtcgg
     3601 tgagaggacg cgtacggatg c
//
LOCUS       DAOSPL010000294         3606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25880_length_3606_cov_2.870177, whole genome shotgun sequence.
ACCESSION   DAOSPL010000294 DAOSPL010000000
VERSION     DAOSPL010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25880_length_3606_cov_2.870177"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..633
                     /locus_tag="PLQ54_06725"
     CDS             55..633
                     /locus_tag="PLQ54_06725"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOS92990.1"
                     /translation="MAECDGTIGETEARKTLLLNVDGGSRGNPGPAGIGVVIREPSGR
                     VVQEISESIGRTTNNVAEYRAMIRGLETCRALNAQRVLVCSDSELLVRQMVGTYRVKA
                     QHLQPLHEQATRLARSFSQVAFRQIPREQNAHADRLASEAIEGRAAASGDTSGEFELV
                     EGDPAIWIQRGSEPGESPPAAGPATDVGRGAS"
     gene            630..1844
                     /locus_tag="PLQ54_06730"
     CDS             630..1844
                     /locus_tag="PLQ54_06730"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOS92991.1"
                     /translation="MKDKCVLAYSGGLDTSVILKWLVETYDYDVIAFCANVGQGDDEL
                     EGIERKALDSGACDVVVRDVREEFVRDFVFPILRAGAVYEGAYLLGTSIARPLLAKEQ
                     IMVARQSGATAVAHGATGKGNDQVRFELTYRAFEPTIRIIAPWRDDQWPIKGRQDAIE
                     YAQRHGIPIKATASKPYSQDGNLLHLSYEGGILEDPWASPPEDMFERTVAPTEAPDTP
                     EEVDIEFEKGTPVAVNGQRLGPAALLERLNEMAGRHGVGRVDMVENRYVGMKSRGVYE
                     TPGGTVIREAHRAVESITMDREVMRIRDRMLPDYAALVYNGYWYSPEMRVLNTFMDAT
                     QENVSGTARVRLFKGNCTILGRKSPVSLYQPQIASFDDDQGAYQQALATGFIELNALR
                     LTVGKMVHGDPL"
     gene            2030..2545
                     /locus_tag="PLQ54_06735"
     CDS             2030..2545
                     /locus_tag="PLQ54_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOS92992.1"
                     /translation="MFEGAKEVQLVVFGLADESYGVHVLAVEEIIRLQEITAIPHAPD
                     FVEGVINLRGRVIPVLDLRKRFGLPPAEATKSTRIVVVEASGMTVGLVVDSVDEVRNL
                     PTDTIEPPSPIVTTVDSDFLMGVGKLPAGGGQEQLVILLDLDRVTSVAERQALSALGA
                     GEEAAALPSGN"
     gene            2711..3511
                     /locus_tag="PLQ54_06740"
     CDS             2711..3511
                     /locus_tag="PLQ54_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS92993.1"
                     /translation="MAKLGLIHYNAPGPDLEGFLNYAAETGFAYVELQCPDVWPEGVD
                     HPEAHAESVRKLVESKGLAVSAVAAHNDFVVLGADEIEFQVTRMQRVCKLAQIVGTSI
                     IRTEGGSPKESVPPDRQVEAMAGCLTRCREFIEPMGIKLAVDNHGWVTNDGDLLLDLF
                     GRVNSPNVGSNLDTMNLRWFGHDLATIDRFYEILAPYVFHTHLKDGTGSRENYVGAAL
                     GDGEIHLTYAVERLKNAGFDGVWCAEYEGREDSAIGYAKCLKWMKANL"
BASE COUNT          685 a         1115 c         1204 g          602 t
ORIGIN      
        1 cattccgacg acgagtagct cacgcgcgtt ggagagccaa gcgacatagc ccgtatggcg
       61 gagtgtgatg gcaccatcgg cgagaccgag gcaaggaaga cgctgcttct gaacgtggat
      121 ggcggatcgc gcggcaatcc cggcccggct gggattgggg ttgtgattcg agaaccatcg
      181 gggcgcgtgg ttcaggagat cagcgagagc atcgggcgca cgacgaacaa cgtggcggaa
      241 taccgggcca tgatacgcgg cctcgagacc tgtcgtgcgc tcaacgcgca gcgcgtactc
      301 gtctgctccg acagcgagct tctggtgcgg cagatggtgg gcacctaccg ggtcaaggcc
      361 caacacctcc agcctctcca cgagcaagcc actcggcttg cccgcagctt ctctcaggtg
      421 gcgttccggc agatccctcg ggaacagaac gctcacgcgg accgcctggc cagcgaggcc
      481 atcgaaggcc gagcggcggc cagcggcgac acttcagggg agttcgagct ggtcgaaggc
      541 gaccccgcta tttggatcca gcggggaagt gagcctggcg aaagcccccc ggccgccggg
      601 cccgcaaccg acgtcggaag gggagcctcg tgaaggataa gtgtgttctc gcgtattctg
      661 gtggcctcga tacttcagtg atcctcaagt ggttggtgga gacatatgac tatgacgtga
      721 ttgcgttctg cgccaacgtg gggcagggcg acgacgagct cgagggcatc gagcgcaagg
      781 cgctcgacag cggcgcttgt gacgtcgtcg tgcgcgatgt ccgcgaggag ttcgtacgcg
      841 acttcgtatt cccgatcctg agagctggag cggtgtacga gggcgcctat ctcctgggga
      901 ccagcattgc ccggccgctg ctggcgaagg agcagatcat ggtggcccgg cagtcgggcg
      961 ccactgcggt ggcgcacggc gccaccggca aggggaacga ccaggtgcgc ttcgagctca
     1021 cctaccgcgc ctttgagccc accatcagaa tcattgctcc ttggcgagat gaccaatggc
     1081 ccatcaaggg ccgccaggat gccatcgagt acgcccagcg ccacggaatc ccgatcaagg
     1141 cgacggccag taagccctac agccaggacg gcaacttgct ccacctgagc tatgagggcg
     1201 ggatcctcga ggacccatgg gcctcgccgc ctgaggacat gttcgagcgc accgtggcgc
     1261 cgaccgaggc ccccgacacc ccagaagagg tggatatcga gttcgagaaa ggcactcccg
     1321 tcgcggtgaa cggtcagcgt ctcggtcccg ccgcccttct cgagcgcctc aacgagatgg
     1381 cggggcggca tggcgtggga cgcgtcgata tggtcgagaa ccgctacgtg gggatgaagt
     1441 cacgaggcgt ctatgagact ccgggcggca ccgtgatccg ggaggcccac cgtgccgtcg
     1501 agtccatcac gatggaccgc gaagtgatgc ggattcgcga ccggatgctg cccgactacg
     1561 ctgcactggt ctacaacggc tactggtact cccctgagat gcgcgtcctc aacacattca
     1621 tggacgccac acaggagaac gtctcgggca cggcccgggt gcggctattc aaggggaact
     1681 gcacaatcct cgggcgcaag tctccggtgt ccctctacca gccgcagatc gcgagcttcg
     1741 atgacgacca gggcgcctat cagcaggccc tggccactgg tttcatcgaa ctgaacgccc
     1801 ttcggctcac cgtgggcaag atggtacacg gagaccccct gtaggccggt cagggcgacg
     1861 agaagtacgt tgtgcgcgtt ccgacccata gttggcgcga ccccgcttcc gacggggagc
     1921 tcaccatggc aggatctcct acaggagact gcgacaccct ctggcgaatg ggaccaggga
     1981 gtgtacccac tgcagtgccc ggtgatgagg aggtagcgcc ttgagcgaca tgttcgaggg
     2041 ggccaaagag gtgcagctgg tggtcttcgg cctggcggac gaatcctacg gggtccacgt
     2101 gctggccgtc gaggagatca tccgccttca ggagatcacg gccattcccc acgcgcccga
     2161 cttcgtggag ggcgtcatca acctacgggg cagggtgatc cccgttctcg atctgcgcaa
     2221 gcgcttcggg ctcccaccgg cagaagccac gaagtcaact cgcatcgtcg tcgtcgaggc
     2281 cagcgggatg actgtgggcc tggtcgtgga ctcggtcgac gaggtccgga atctcccgac
     2341 ggacaccatc gagccgccct cgcccatcgt gaccacggtg gactcggact tcctgatggg
     2401 cgtgggcaaa ctccctgccg gcggcgggca ggaacagctg gtgatcctgc tcgatctcga
     2461 ccgggtcacg agcgttgccg agcggcaggc cctgtcagcc ctgggggcag gagaggaagc
     2521 tgcggcgttg ccgtcgggca actaagcagt ccctctcaca accggtctcg acatgcatcg
     2581 cgacgcacgg ccagctgcaa cggggctggc cgcacaaggt gtatgggcgc gttcgcccga
     2641 atgccctgta gcctgccctg cagcctcgcg gggcgggctt cactttcgag ccgatcaagg
     2701 aggatgaccc gtggccaagc tagggttgat ccactacaac gctcccggcc cggacctgga
     2761 ggggtttctc aactacgccg cggagacggg cttcgcctat gtcgagctgc agtgccctga
     2821 tgtctggccg gagggagtcg accaccccga ggcccatgcc gagagcgtcc ggaagctcgt
     2881 ggagtccaaa ggcctggccg tctccgccgt agcggcacac aacgacttcg tcgtgctcgg
     2941 agctgacgag atcgagttcc aggtcacccg gatgcagcgc gtctgcaaac tcgcacagat
     3001 cgtcgggacg agcatcattc gtaccgaagg tggatcgccc aaagagagcg tcccccccga
     3061 ccggcaggtc gaagccatgg ccgggtgtct gacgcgctgc cgggagttca ttgagcccat
     3121 gggcattaag ttggccgtcg acaaccatgg ctgggtgacc aacgacggag acctgctact
     3181 ggacttgttt gggcgcgtaa actcaccgaa cgtcgggtcc aacctcgaca ccatgaacct
     3241 gcggtggttc gggcatgacc tggcgaccat cgaccggttc tatgagatcc tcgcccctta
     3301 cgtgtttcac acacatctca aagatggcac gggcagccgc gagaactatg tgggagccgc
     3361 tttgggtgat ggcgagatcc acctgaccta cgccgtcgag cggctgaaga atgccggctt
     3421 tgacggtgtc tggtgcgcgg agtatgaggg gcgggaagac tcggccatcg gctatgccaa
     3481 gtgcctgaag tggatgaaag ccaacctgta gccgcgtcgc ggtgcgggag gtcgaggtcg
     3541 aatgagcggc tgggatcggg tccacatgac ggtgttcacc aagccgtgga agacgctgac
     3601 catacc
//
LOCUS       DAOSPL010000295         3599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25941_length_3599_cov_2.928612, whole genome shotgun sequence.
ACCESSION   DAOSPL010000295 DAOSPL010000000
VERSION     DAOSPL010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25941_length_3599_cov_2.928612"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3246
                     /locus_tag="PLQ54_06745"
     CDS             <1..3246
                     /locus_tag="PLQ54_06745"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOS92994.1"
                     /translation="LFRQGEAHTITFSIDPETVPDGIDEVTLILSRSDGRGHPAPVPP
                     EARLLRAEGFRATLAVPDEPGFSRLSYWPTFLAPGDRRNLARGTLDYFVQGSPAAEET
                     TAVQVAEPMHWTPSAVVSLDGEGWLLATDATNAGVDAGWFNAPVPEAKPTKVPWIIQD
                     PFVGYHGVAWYWRRFNAPANPHAGGRTLLRFGAVDYKADVWVNGQPVGGHEGGETPFE
                     LDITNAVRIAEPNLLAVRVLNPTHEPIDGITLNEAPRRAKVIPYSAGASYNHGGIVES
                     VVLASVPQAYISDLYLRPDTASGRVVAEATIHNAASEALTGTLSLLVAPAASGTTLDA
                     RAQAVELPPGDSVVRTSLSVGVPHLWDLADPYLYRVTARVVVDGSGDERSDRCGFRDF
                     RFENGAFRLNGRRVLLRSAHTVNSTPVGQQVPGDPALFQRDILFMKTMGFNCIRFIWG
                     GATRRQLDMCDEMGLMAYVEHAASNPMAPSPQMAGRFDRSVAETIRRDRNHPSVVMWG
                     LLNETPDGPVFRHATDILPLVRSLDETRVVMLNSGRWDGQLEIGSLANPGGLGWDTYL
                     GEEGPDGGVTTWGQFGGYFPRAGDIHVYPRVPHTTGILDGLRRLGEGGGPVFLSEYGI
                     GSAVDLWRVTRHFEQLGKPEAEDALYYRERLNRFLADWDQWRLSECFISPQGFLAESL
                     RKMARQRTLGLNIIRANPRIVAYNLTGMFDHVNCGEGLFTLFRELKPGTTDALFEGLA
                     PLRLCLFAEPAHVRRGGRVALEGVLASEDVLAPGTYPVRLQVIGPDMARVLDRVVDVV
                     VPPTTPEGEPPLAIPFFRDEVTVDGPGGPYRFMATMESGGAPTGGDAAFTVWDVADLP
                     AVEAEVTLCGDSPGLREWLAARGVRVREYGNGPAPGRAVILVAGKPGADDAGAWRDLA
                     RRVASGATALFLRPDVFRRGNDPTGWLPLERKGTIRAIYGWLYLKDEWAKAHPIFHGL
                     QSGGLMDYDLYRDIIPDLVLSGQDAPDDAICGAIKASQDYDSGLMVAEYRLGAGRFLV
                     STLQILENLPRDPVAERLLANALVWAADEQGEAPAPLPEGFEEQLDRFGYQ"
     gene            3406..>3599
                     /locus_tag="PLQ54_06750"
     CDS             3406..>3599
                     /locus_tag="PLQ54_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92995.1"
                     /translation="MPLPEGFEERLNRFATGKGRLVPPGTASRVCDHTARARHGLPAH
                     SPALGRAEEQGIGSVRWPATV"
BASE COUNT          540 a         1213 c         1278 g          568 t
ORIGIN      
        1 ctgttccggc agggtgaggc ccacacgatc acgttcagca tcgatccgga gacagtcccc
       61 gacggcatag acgaggtcac gctgatcctc tcgcgaagcg acggccgtgg ccatcccgcg
      121 ccggtccccc ctgaggcgcg actcctcagg gccgagggct tccgcgccac gttggcggta
      181 cccgacgagc cgggtttctc gcgcctctcg tactggccca cgttcctcgc tcccggcgac
      241 cggcggaacc tggccagggg gacgctggac tacttcgttc agggctcgcc cgcggcggag
      301 gagaccaccg ctgtgcaggt agccgagccc atgcactgga cgccgagcgc cgtggttagc
      361 ctggacggcg aggggtggct gctcgccacg gacgcgacca acgcgggggt cgacgctggc
      421 tggttcaacg cgcccgtgcc tgaggccaag ccaaccaagg tgccgtggat cattcaggat
      481 ccgttcgtcg gttaccacgg cgtcgcctgg tactggcggc ggtttaacgc accggcgaac
      541 ccgcatgccg gcggacgcac gctccttcgt ttcggcgccg tggactacaa agccgatgtg
      601 tgggtcaacg ggcagccggt gggggggcac gagggcggcg agactccctt cgagctcgat
      661 atcaccaatg cggttcgcat cgccgagccc aacctgctag ccgttcgcgt gctgaacccc
      721 acgcacgagc ccatcgacgg catcacgctg aacgaggcgc cacgccgggc caaggtgatc
      781 ccctacagcg ccggggcctc ctacaaccat ggcgggatcg tcgagagcgt ggtgctggcg
      841 tcggtccctc aggcgtatat cagcgacctg tacctgcgcc cggacacagc gtcgggccgc
      901 gtcgtggcag aggccaccat ccacaacgcg gcgagtgagg cgctgacggg cacgctatcg
      961 ctcctcgtgg cgccggcggc ttcggggacc acgctcgacg cccgcgcgca ggcggtcgag
     1021 ctgccccccg gcgacagtgt ggtccgcacc tcgctgtcgg ttggcgtacc gcacctgtgg
     1081 gatctggccg acccgtacct ctaccgcgtg actgcccgcg tcgtcgtgga cggctcgggc
     1141 gatgagcgtt cggatcgttg tgggttccgc gacttccgct tcgagaacgg agccttccgg
     1201 ctgaacggtc gccgtgttct cctccgcagc gcgcacaccg ttaacagcac gcccgtgggg
     1261 cagcaggtcc ccggcgaccc ggcactgttc cagcgcgaca tcctgttcat gaagaccatg
     1321 ggcttcaact gcattcggtt catctggggc ggagcgacac ggcggcagct tgacatgtgt
     1381 gacgagatgg gcctgatggc gtatgtcgag cacgcggcct cgaacccgat ggccccgtcg
     1441 ccgcagatgg ccgggcggtt cgaccgctcg gtggcggaga ccatccggcg agaccggaac
     1501 caccccagtg tggtcatgtg ggggctgctg aacgagacgc cggacgggcc ggtgttccgt
     1561 catgctacgg acatcctacc cctggtgcgg tcgctggatg agacgcgcgt ggtaatgctc
     1621 aacagcggcc ggtgggacgg ccaactcgag atcggcagcc tggcgaatcc cggcggcctg
     1681 ggttgggaca cctatctagg cgaggaaggc cccgatgggg gcgtcacgac gtggggccag
     1741 ttcggcggct acttcccgcg ggccggggac atccacgtct acccgcgggt gccccacacg
     1801 acaggcatcc tcgatggcct gcgcaggctg ggcgagggcg ggggaccggt cttcctgtcc
     1861 gagtatggca tcggcagcgc ggtggacctc tggcgcgtga cccggcactt cgagcagctg
     1921 gggaagcccg aggcggaaga cgccctgtac taccgcgagc gtctgaatcg tttcctggcc
     1981 gattgggacc agtggcggct gtcggagtgc ttcatatcac cacagggttt cctcgccgag
     2041 agcctacgga agatggcgcg tcagcggacc ctggggctca acatcatccg ggccaaccca
     2101 cgcatcgtgg cctacaacct gaccggcatg ttcgaccacg tgaactgcgg cgaggggctc
     2161 ttcaccctgt tccgggaact gaaaccgggc acgaccgatg ctctgttcga ggggctggcg
     2221 ccgctgcggc tgtgcctgtt cgccgagccg gcccatgtgc gtcgaggagg gcgtgtagcc
     2281 ctcgagggag tgctcgccag cgaggacgtc ctggccccgg gcacctaccc ggtgaggctg
     2341 caggtgatcg ggccggacat ggcccgggtg ctcgaccgcg tcgtcgacgt cgtggttcct
     2401 ccaacgacgc cggaaggcga gccgccgttg gccatcccct tcttccggga cgaggtgacc
     2461 gtggatggcc cgggtggtcc gtaccgcttt atggccacga tggagtcggg tggggcaccc
     2521 actggcggcg acgcggcgtt caccgtgtgg gacgtcgctg atctgcccgc ggtggaggca
     2581 gaggtgactc tgtgcggcga tagccccggt ctgcgtgaat ggcttgccgc tcgcggcgtg
     2641 cgtgtgcgcg agtacggcaa cggccccgcg ccgggccgcg cggtgatcct ggtggccggg
     2701 aagcccggcg ccgatgacgc cggggcctgg cgcgacctgg cccgccgcgt tgcgtccggt
     2761 gcgacggcgc tgttcctcag acccgacgtg ttccgacggg gcaacgaccc gacgggctgg
     2821 ttgcccctgg agcgcaaggg caccattcgc gccatctacg gctggcttta tctcaaagac
     2881 gagtgggcca aggcgcaccc gatcttccat ggcctgcagt cgggtgggct gatggactac
     2941 gacctctacc gggacatcat ccccgacctc gtactctccg ggcaggacgc gccggatgac
     3001 gcgatctgcg gcgccatcaa ggcctcacag gactacgact cggggctgat ggtcgctgag
     3061 taccggctcg gcgcggggcg attcctggtc agcacgctgc agatcctcga gaatctcccc
     3121 cgcgacccgg tggccgagcg gctgctggcc aacgcactgg tgtgggccgc tgacgaacag
     3181 ggggaggccc cggcgccgct gccggagggg tttgaggagc agctggaccg gttcggctac
     3241 cagtagggac ccgctggtgg tagctcaggc ccgggccgca gagcgtccgg gctctgcggg
     3301 aagctcagga cagccgggtc ccagctgcgg ccggaacgcc gccagccgcc gatgcccctg
     3361 tcaggtgcct ccgcgcgacg aggcagacca cgtgacccgg ccccagtgcc gctccccgag
     3421 gggttcgagg agcggctcaa ccggttcgcg accggtaagg gccggctggt gccaccaggc
     3481 acggcatcgc gagtgtgtga ccacacggca cgagcacgcc acggcttgcc agcgcattcg
     3541 cccgctctcg gccgcgcgga ggaacagggc atcggttcag tgcgttggcc cgccacggt
//
LOCUS       DAOSPL010000296         3596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_25972_length_3596_cov_2.988139, whole genome shotgun sequence.
ACCESSION   DAOSPL010000296 DAOSPL010000000
VERSION     DAOSPL010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25972_length_3596_cov_2.988139"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="PLQ54_06755"
     CDS             <1..1116
                     /locus_tag="PLQ54_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOS92996.1"
                     /translation="RGVTIPASEAVRILLVDDDEDDFVMTRALLAEVTGSIRFRLEWA
                     PTYDQGRALILEGRHDVYLLDYQLGGRDGLELLQEAIAAGCKAPIILLTGQGDDAVDR
                     DAMKAGAADFLVKGQADAHLLERSIRYALERRRIEQMKDRLLALASHELRTPLTAIKG
                     YALTILQGGALAPDALETECLQSIIQGADHLTRLIDGFLDLSRVEAGRAIDLVCEDFD
                     VRAMVDEAVGIAAMAARRCTYVVACDEGIGALHGDRYKLLLVLTNLLSNADKYSPGGG
                     EVTLRVTRAPGEVRFAVEDHGMGIPADAHDALFTPFHRVRDPRALDIRGTGLGLSLSK
                     HLVEAHGGRIWIDSEVGRGTTVSFTIPARTSPAPQSA"
     assembly_gap    1200..1299
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1557..2894
                     /locus_tag="PLQ54_06760"
     CDS             1557..2894
                     /locus_tag="PLQ54_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92997.1"
                     /translation="MAQVPDRLQHQSAQLDVSRAGGHSGRWTSCLTWVAVGFVLLLVA
                     AYVALIAAFGVHTGRGWIDSNRKTIAPYSKPVVAGNGLDVYTNAAAATDAVRSSLPLP
                     AAAPAPSSAPLDTWDDAIASALSGTGDVGRCAPVLRASSRALSLLHDAADKPYVSPIV
                     PEYDAKLPHLAQFRQLARLGAARVRYLHLRGQDAEALAVARDVLALGVNMPQRGAMVE
                     LLVGDVCIEIGHRQAMAVIEQSDLPAVEYLAHARYVRKLRERVYPLGKAIELEYHWAA
                     ELAEEVRRGNSAVLRDFGWDSDDGLDAARRRARLALWHPKQSLPWIEDRLARLAEVAD
                     ALYNSPEYAQIDGRTEDDLVARNDWFAELIVPVFGKARGRHAEARAPWPSRRWPPYWP
                     PTGLSMGTTRRRCNRSCRGTRRISPMTHSTARRSATNGRERATGCGRRAWTRT"
     gene            3087..>3596
                     /locus_tag="PLQ54_06765"
     CDS             3087..>3596
                     /locus_tag="PLQ54_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS92998.1"
                     /translation="MSGALLLFPVSLCAMAMGQTSPWEADFARQPVSSRPRLETRYVT
                     SARTAAEVLAQTYQLRLDFGSPAGHRATAHTTDGTQFAELWLEVEAADGTVYSSRRSR
                     DRSRINLYRRGPYCHEVHWFDVTCTTGEGKVLPVRGEIVLHCYPEKAHMQAILHAEGE
                     RQARILTAEG"
BASE COUNT          525 a         1195 c         1212 g          564 t
ORIGIN      
        1 aggggtgtga ccattccagc ctcagaggcc gtgcgcattt tgttggtgga cgacgatgag
       61 gacgacttcg tcatgacccg cgcgctcctg gccgaggtga cgggcagcat ccgcttccgg
      121 ctcgagtggg cgccgaccta cgaccaaggg cgggcgctca tcctcgaggg ccggcacgat
      181 gtgtatctgc tcgactacca gttgggcggg agggacggcc tcgagctgct ccaggaggcc
      241 atcgcggccg gctgcaaggc gcccatcatc ctcttgaccg gccagggcga cgatgccgtc
      301 gatcgtgacg ccatgaaagc gggcgccgcg gacttcctcg tcaaaggtca ggcggacgct
      361 cacctgctcg agcggtcgat ccgctatgcc ctcgagcgcc ggcggatcga gcagatgaag
      421 gaccgcttgc tggccctggc ctcgcacgaa ctccgcacgc ccctgacggc catcaagggc
      481 tacgccctga ccatcctcca gggaggcgct ctggcgcccg acgcgctcga gaccgaatgc
      541 ctgcagagca ttatccaggg cgccgatcat ctgacccggc tcatcgatgg cttcctggat
      601 ctgtcgcgag tcgaggccgg ccgcgcgatc gatctggttt gcgaggactt cgatgtccga
      661 gccatggtgg acgaggccgt cgggatcgcc gccatggcgg cgcgccggtg cacttacgtg
      721 gtggcatgcg acgagggcat cggggctctg cacggcgacc gctacaagct cctgctggtg
      781 ctcacgaacc tgctgtccaa cgcggacaag tactcgcccg gcggtggcga ggtcaccctc
      841 cgcgtgaccc gggccccggg cgaggtgcgg ttcgcggtcg aggaccacgg catggggatc
      901 cccgccgacg cgcacgatgc gctgttcacc cccttccacc gtgtccgcga cccgcgggcg
      961 ctggacatcc gtgggacggg gctcgggctg tcgctgtcga agcacctggt tgaggcccac
     1021 ggtggtcgca tctggatcga ctccgaggtc ggcaggggaa cgaccgtgtc gttcacgatt
     1081 ccggcgcgaa cgagcccggc tccgcagtcc gcctgacgca cgggtcccct tcgcgcgatc
     1141 catgcccgcg ccgttgccct ggcgggcgag gacgcccgcc ggcacaggac atggtcgttn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cccgccggca caggacatgg
     1321 tcgttcgtgc ctgcggggct cctgccccgc gccgcatccg cggagacggc cgtgtgcttc
     1381 cgcctgcagc ggcgaagcgc cttcacccga gcgcccgcgc acccatcctg ggcgcatctt
     1441 ggcgcatctg attcgccgag ggtgggacat ggttgacgct tcagatgctg tatggttgtg
     1501 aggcactcaa ccaaagagga tccgctctcc gtttggtccc tgggagagga cgcgccatgg
     1561 cccaagttcc cgatcggcta caacaccagt cggcccaact cgatgtcagt cgtgcagggg
     1621 gtcactcggg ccggtggacc tcgtgcctga cttgggtcgc cgtcggcttc gtgcttttgc
     1681 tggtcgctgc ctacgtggca ctcattgctg cgttcggcgt acacaccggc agaggctgga
     1741 tcgattcgaa tcggaagacg atcgctccgt acagcaagcc cgtggtggct ggcaatggtc
     1801 tagatgtcta taccaatgcc gccgcggcca cggatgccgt ccgctcgtcg ctgccgctcc
     1861 cggcggcggc accagcgcca tcctcggcac cgctcgacac ctgggatgat gccatcgcct
     1921 ccgcgctctc gggcacgggt gacgttgggc gctgcgcccc ggtgctccgc gccagctcgc
     1981 gggccctgtc gctgttgcac gacgcggccg acaagcccta tgtgtcgccc atcgtgccgg
     2041 agtacgacgc gaagctgccc catctcgctc agttccgcca actcgcacgc ctgggggcgg
     2101 cgcgggtgcg gtacctacac ctgcgaggcc aggacgctga ggccttggcc gtggcccgcg
     2161 atgtgctggc cctcggcgtg aacatgcccc agcgaggggc gatggtcgag ctgctggtcg
     2221 gggacgtgtg catcgagatc ggtcatcggc aggcgatggc ggtgatcgag cagagcgacc
     2281 tccccgccgt ggagtacctg gcccacgcgc ggtacgtgag gaagctcagg gagcgtgtct
     2341 accccctggg caaggcgatc gagctcgagt accactgggc cgcagagctg gccgaggagg
     2401 tgcgccgagg gaactcggcc gtgctgaggg acttcggctg ggattcggac gacgggctcg
     2461 atgcggcacg caggcgcgcc cgcctcgcac tgtggcatcc caagcagtcg ctgccgtgga
     2521 tcgaggaccg cctggcccgg ctggccgagg tggccgacgc gctgtacaac agcccggaat
     2581 acgcgcagat cgacggccga accgaggacg acctggtggc tcgcaacgac tggttcgccg
     2641 aactgatcgt ccccgtgttc ggcaaggcgc gcggccgaca cgccgaggca cgtgctccct
     2701 ggccctcgag gaggtggccg ccctactggc cgcctaccgg gctgagcatg ggcactaccc
     2761 ggagacgctg caaccgctcg tgccggggta cgcgaaggat ctccccgatg acccattcga
     2821 cggcacgccg ttccgctacg aacggacgag aacgggctac cggctgtggt cgccgggcat
     2881 ggacgcgtac ctagccgccc gccgcgctgc agcggcggcg accggcgcgc ccgcgccggc
     2941 gaaggcccct ccttacccgc cgacgaaggc gctgtagcgg gaagtggatg agcgtcggca
     3001 cgggcggcga gggcatcatc tctcggggct cctcgtagcc gcaacggtgc tccctagcca
     3061 ggctccacgg tacggaggcg caggatatga gtggtgctct tctgcttttt cccgtgtctc
     3121 tctgtgcaat ggcgatgggg cagacgtcgc cgtgggaggc ggatttcgcg cgccaacctg
     3181 tctcatcacg cccgaggctt gagacgcgct acgtcacttc ggcccgcacg gcggctgagg
     3241 tgctcgcgca gacctaccag cttcgtctcg acttcggctc cccggccggg caccgggcaa
     3301 cggcccacac caccgacggg acgcagttcg ccgagctgtg gctggaggtg gaagccgctg
     3361 acggcaccgt ctactcctcc cggcggtcac gcgaccggtc tcgtatcaac ctgtaccgcc
     3421 gggggccgta ttgccacgag gtccactggt tcgacgtgac gtgcacgacc ggcgagggga
     3481 aggtccttcc ggtcaggggc gagatcgtgc tgcactgcta ccccgagaag gcccacatgc
     3541 aggcgatcct gcacgcggag ggcgagcggc aggcgcgcat cctcaccgcg gagggc
//
LOCUS       DAOSPL010000297         3588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26056_length_3588_cov_2.745259, whole genome shotgun sequence.
ACCESSION   DAOSPL010000297 DAOSPL010000000
VERSION     DAOSPL010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26056_length_3588_cov_2.745259"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="PLQ54_06770"
     CDS             <1..1047
                     /locus_tag="PLQ54_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOS92999.1"
                     /translation="IRDAADKARDMRRFHGRRTILFVDEIHRFNKAQQDAFLPVVEDG
                     TFILIGATTENPYFEVNAPLISRSTVLRLEALAVEDIRGLLERALADAERGLGANPVH
                     VDDDAMDHLVLLSNGDARRALNALEQAAMTATLVDGERHVDLECARAATQARALTYDK
                     TGDQHYDVISAFIKSMRGSDPDAAVYWLARMLAAGEDPLFIARRIVICASEDVGNADP
                     MALVVANAAAQAVQFVGLPEGRIPLSQAAIYVACAEKSNAAYLAIDRATADLQKRPPL
                     PVPLHLRNASFEAQRQAGISVGYQYPHDFPRGWVAQSYLPDGMEDVRYYEPTDRGREA
                     RLAERLRALRGDRD"
     gene            1089..2012
                     /locus_tag="PLQ54_06775"
     CDS             1089..2012
                     /locus_tag="PLQ54_06775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOS93000.1"
                     /translation="MRWIWQRRWWVLAALVIVALIVRAQLRSAAHQVSTAEAIRGALY
                     LEIAASGRVEAPGADLGFRGSGELIGLYVDEGQRVTASQRLARIARFDPPSGGADSGD
                     VIRAPYDGRIVEIYQRTGAVVAAGNPVLRITADQGAWVTAFVNSEDALYLNRGDHLLC
                     RVGGYLSEPWPITVEAVGSEAVQRRDLVGSAAQVRVRCRIDSPSNTPPAGTEVDVDGR
                     VCLLADGVLIPAAAVVHDGPEDRVWVVETGTAHPRAVTLGRNNFDQIEIRHGIEPGEI
                     VIVRGKPGLTDGMRVRAKPMPPEAEQVSGGE"
     gene            2016..2738
                     /locus_tag="PLQ54_06780"
     CDS             2016..2738
                     /locus_tag="PLQ54_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS93001.1"
                     /translation="MSAPGTATGSALTARGLWKRYAMGDVVVEALRGVHLDLPAGRFV
                     VLLGPSGSGKTTLLNLAGGLDVPSEGSLCVCGHELHGMNEAQLTEFRRTTVGFVFQLF
                     NLVPTLTALENVRLVAQLVGRDDYARTVLEAVGLAGQAHHLPSQLSGGEQQRVAIARA
                     LVKQPRLLLADEPTGALDAETGRRVISLLWEQTRESDMTVLMVTHNEDLAGMGDLVVR
                     LRSGEVVEVSDGPAARLREAPP"
     gene            2735..>3588
                     /locus_tag="PLQ54_06785"
     CDS             2735..>3588
                     /locus_tag="PLQ54_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93002.1"
                     /translation="MSGNVPPRVPMLARKIVADTRYLGRRALGLVVLMGLGSMLFTGL
                     YQSYQNLTTSYGYVYRVGRFADASVLVDSAPEGLVRAAEGIPHVLSAIGRPARDGAIK
                     QPGRPHERAMGRFIGVPRGRRPKINDLLLTEGRYIRGVDEAVLEQQFAQANDYELGDR
                     IQGVYRATEREFTIVGFATSPEYLYPVPSEEMSLAMPGTFGVLFIDDDRAREWFGLGR
                     RITEIHCRTDPGHQREVLAKLKGLANPYGLQTGYIQDDQPSKKLLTMDQQGLATLSVF
                     FPVLFLGAS"
BASE COUNT          561 a         1304 c         1165 g          558 t
ORIGIN      
        1 atccgcgacg ccgccgacaa ggcccgcgac atgcggcgct tccacggccg gcgaaccatt
       61 ttgttcgtcg acgagatcca ccggttcaac aaggcccagc aggacgcgtt cctccccgtg
      121 gtcgaggacg gtaccttcat cctgatcggt gcgacgaccg agaaccctta cttcgaggtc
      181 aacgcgcccc tgatctcgcg ctccaccgtg cttcgactcg aggctctcgc cgtcgaggac
      241 atccgagggc ttctggagcg cgccctggcc gacgccgagc gcgggctggg ggcgaacccg
      301 gtccacgtcg acgatgatgc catggaccat ctggtgctcc tatccaacgg tgacgcccgc
      361 cgcgcactca atgccctgga gcaggccgcc atgacggcta ctctcgtcga cggcgagcgg
      421 cacgtggacc tcgagtgcgc gcgggccgcg acgcaggccc gggccctaac ctacgacaag
      481 accggcgatc agcattacga cgtcatctcg gccttcatca agagcatgag gggctccgat
      541 cccgacgccg ccgtctactg gctggcgcgg atgctcgcgg caggcgagga ccccctcttc
      601 atcgcgcgcc gcatcgtcat ctgcgcgtcc gaggacgtcg gcaacgccga tcccatggcc
      661 ctcgtcgtgg ccaatgccgc ggcccaggcg gttcagttcg ttgggctccc cgagggacgg
      721 atccccctct cccaggctgc catctacgtc gcctgtgccg agaagagcaa tgcggcctac
      781 ctcgccatcg accgcgcaac cgctgacctg cagaagcgtc ctcctctacc ggtgcctctc
      841 cacctgcgca acgcctcctt cgaggcccag cgccaggccg gcatcagcgt cggataccag
      901 tatccgcacg acttccctcg cgggtgggtc gcccagagct acctacccga cggcatggag
      961 gatgtgcgat actacgagcc aaccgatcgc ggccgtgagg ccagactcgc agagcgcctc
     1021 cgggcgctcc ggggcgatcg cgactagccg cgcgccggac gcatcacgcc cgggagcgga
     1081 ggaatggcgt gcgctggatc tggcaacgaa gatggtgggt cctcgccgct ctcgtcatcg
     1141 tcgcgctcat cgtgcgcgca caactccggt ctgccgcaca ccaggtctcc acggctgagg
     1201 ccatccgcgg agctctctac ctcgagatcg ccgcctcggg acgcgtcgag gcacccgggg
     1261 ccgacctcgg gttccgaggc tcgggggagc tcattgggct ctacgtcgac gagggccagc
     1321 gtgtgaccgc ctcccagcgc ctcgcccgca tcgcgcggtt cgatccgccc tccggtggcg
     1381 ccgactccgg cgacgtcatc cgcgcgccct acgacggccg catcgtcgag atctatcagc
     1441 gaacgggcgc cgtcgtcgcc gccggcaacc ccgtgctccg catcaccgcc gaccagggcg
     1501 cgtgggtcac cgccttcgtc aacagcgagg acgccctcta cctcaaccgc ggcgaccacc
     1561 tcctgtgccg cgtggggggc tatctgtccg agccatggcc catcaccgtc gaggccgtgg
     1621 ggagtgaggc cgtccagcgg cgcgacctcg tcggctcggc ggcccaggtc cgggtccggt
     1681 gccgcatcga cagcccctcg aacaccccac cggccggaac cgaggtcgat gtcgatggcc
     1741 gcgtgtgcct actggctgac ggggtgctca tcccggccgc cgccgtggtc catgacggcc
     1801 ccgaggaccg cgtctgggtc gtcgagacgg gcactgcgca tccacgggcc gtgactttgg
     1861 ggcgcaacaa cttcgaccag atcgagatcc gccacggcat cgagccgggc gagatcgtca
     1921 tcgtgcgcgg caagcccggc ttgaccgacg gcatgcgcgt ccgggcgaag cccatgccgc
     1981 ccgaggcgga acaggtgtcg gggggcgagt agccgatgag cgcccccggg accgcgacgg
     2041 gctcagcact caccgcccgt ggcctgtgga agcgctacgc catgggcgac gtggtcgtcg
     2101 aggccctccg cggcgtccat ctcgacctgc cagccgggcg gttcgtcgtg ctgctcggcc
     2161 ccagcggatc cggaaagacc acgctcttga accttgccgg cgggctcgat gtgccctctg
     2221 agggctcgct ctgtgtctgc ggccacgagc tccacggcat gaatgaagcc cagctcaccg
     2281 agttccgccg aaccacggtt ggcttcgtct tccagctgtt caacctggtg ccgaccctga
     2341 ccgcgctcga aaacgttcgc ctggtcgccc agctggtcgg gcgcgacgac tatgcccgga
     2401 ccgtactcga ggccgtgggc ctggccggcc aagcccatca cctgccatca cagctgagcg
     2461 gcggcgagca gcagcgcgtc gccatcgcgc gcgcattggt caagcagccg cgactgctct
     2521 tggcggatga gcccacgggg gccctcgacg cagaaaccgg ccgccgtgtg atctctctgc
     2581 tgtgggagca gacccgagag agcgacatga cggtgctgat ggtgacccac aacgaggatc
     2641 tggctggcat gggcgatctc gtggtgcgcc tccgcagcgg ggaggtcgtc gaagtctctg
     2701 atgggcccgc ggctcggctc agggaggcgc ccccgtgagc ggcaacgtcc cgccgcgcgt
     2761 gccgatgctc gcgcgcaaga tcgtggctga cacccgctac ctgggtcgga gagccctcgg
     2821 cctcgtggtg ctcatgggct tgggctccat gctgttcacg gggctgtacc agtcatacca
     2881 gaacctgacg acaagctacg gctacgtcta ccgtgtgggt cgcttcgccg atgcatccgt
     2941 tctggtggac tcggcccccg agggactcgt gcgcgccgcc gagggcatcc cccacgtcct
     3001 cagcgccatc ggccgtccgg cgcgcgacgg tgccatcaag caacccggcc gaccccacga
     3061 gagggccatg ggccggttca tcggagtgcc ccgcggccgc cggcccaaga tcaacgacct
     3121 gctgttgacc gagggccgct acatccgcgg ggtcgacgag gcggtactcg agcagcagtt
     3181 cgcccaggcg aacgactacg agttgggtga ccgcatccag ggcgtctatc gtgcaaccga
     3241 gcgggagttc accatcgttg gcttcgcgac cagccccgag tacctctatc ccgtcccctc
     3301 cgaggagatg tccctggcca tgccgggaac cttcggcgtg ctcttcatcg acgatgaccg
     3361 ggcccgcgag tggttcgggc ttgggcgcag aatcaccgag atccattgtc gcaccgaccc
     3421 cggacaccag cgcgaggtgc tggccaagct caagggcctg gccaacccct acggcctcca
     3481 aacgggctac atccaggacg accagcccag caagaagctg ctgaccatgg accagcaagg
     3541 actggccacg ctcagcgtgt tcttccccgt gctgttcctg ggggcttc
//
LOCUS       DAOSPL010000298         3581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26127_length_3581_cov_2.939592, whole genome shotgun sequence.
ACCESSION   DAOSPL010000298 DAOSPL010000000
VERSION     DAOSPL010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26127_length_3581_cov_2.939592"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1732)
                     /locus_tag="PLQ54_06790"
     CDS             complement(<1..1732)
                     /locus_tag="PLQ54_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93003.1"
                     /translation="MKLKLWGFATLLIVVAASAVLAVPGDTTGTAVRLEHEEALGAAA
                     DAAAAVGASDTATSLADLEGLGDEPVSLELQDVPVSQVLRAVAEWRRLPIMIGADVKG
                     EIGYLSIPSATADEVLQAILGNPELRLASETRNGILCVYSKDSEAGRTVAERAGTGVA
                     ASDAPELRRAAEVLNVSAAPDLKLQPKGSVEGIRSASDAIGAGASGGEQKAEEPRHYE
                     VIEVKYADLPTVVAAVGGTVVSTGPRGLGPLQEAQMAALRREGMRGSKFASANINRMA
                     ALNGVGGAGYGQFGGWGGGGNFGGGGFGGRGGGGWGGGGFGGGGLGGFGGGGLGGFGG
                     GFGGGYGGGGNLVLPDDVDSIQAVLELNSVIVHGAQQGIDQVRELIEILDQPIKQVAV
                     ELIVADVSMSDTKSKAIRVSFTDGTVEIGSNPAATTGGSLSIGYVNGDFRAALSANKL
                     ETVADVLQMPRVITQNMTPAFIGEYRTVPVVIPTLTRDAFGNVIQGLEQVDELEIGVM
                     FNITPRINADNSVTMYVVPEISVPGETLVVNGTSIPTESEQYIETVVQVPDGKTVVLG
                     GVIDRSRSVSR"
     gene            complement(1934..2824)
                     /locus_tag="PLQ54_06795"
     CDS             complement(1934..2824)
                     /locus_tag="PLQ54_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93004.1"
                     /translation="MRRFLPGLTLVAVGLLVCGGTAFAQASNDLITVKCYDEGLKEVV
                     QAIAKFGLPKIEFAKDVRDQDVTVLLSEVTPAVALQFILDKYGLEKVEADGGLTIRNK
                     AGATNKRAEGASEGKGKSVLAGDDSEIVYRVAPLQIAQGSGMMGGGTTNRRGGTSSGR
                     TGRSGGLGLGAGGGTGPGAGGQGGTASEEETEDYLYEVIEAKHIGGYSAALLFGGGYV
                     LMNTDVMQMGGGGYGGGGYGGGYGGGGYGGGGYGGRGGGYGGRGGGYSSNRGGNSWGG
                     NRGGGGWGGNRGGGGWGGGW"
     gene            complement(2952..>3581)
                     /locus_tag="PLQ54_06800"
     CDS             complement(2952..>3581)
                     /locus_tag="PLQ54_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93005.1"
                     /translation="EGAATMQGQLAVTDFGDPLFPFKENPFASKRSSLSASLPMHDPA
                     DVFAERGFPRPRKVRQPVLAGPEPIGIQPAGWFVPNPTIPRAQPPKLGGESVRPVVGT
                     SQEEAPRVVAGGAQSVGAVGPRVTRKPPDWSFRRVSGILHDGVSLAIIEVYEGDKPVG
                     RIVKPGDRIRAGGRQFTVRAIGADSVSLRETGSTEDVVVRLRGVTANEG"
BASE COUNT          596 a         1338 c         1073 g          574 t
ORIGIN      
        1 ccctgctgac gctgcgggat cggtcgatga cgccgccgag cacgaccgtc ttgccgtccg
       61 gcacctgcac aaccgtctcg atgtactgct cggactcggt cgggatggaa gtcccgttga
      121 cgaccagcgt ctcgcccgga actgagatct cgggcaccac atacatggtc accgagttat
      181 ccgcattgat gcgcggggtg atgttgaaca tgacgccgat ctcgagttcg tcgacctgct
      241 ccaggccctg gatgacattc ccgaaggcgt cgcgcgtcag tgtagggatt accaccggga
      301 ccgttcggta ctcgccaatg aaggcggggg tcatgttctg cgtgatgacc cgtggcatct
      361 ggagaacgtc cgcaacggtc tcgagcttgt tcgccgagag tgccgcccgg aaatcgccat
      421 tcacgtagcc gatcgataga ctgccgcccg tcgtcgctgc cgggttagac ccgatctcca
      481 ccgtcccgtc agtgaagctc acccggatgg ccttgctctt cgtgtcgctc atcgatacat
      541 cagccacgat aagctctacc gccacctgct tgatcggctg atccagaatc tcgatgagct
      601 cgcggacctg gtcgatcccc tgctgggcac cgtgaacaat gaccgagttc agctcgagga
      661 cggcctggat gctgtcgacg tcatccggga gcaccaggtt gccgccgccg ccgtagccgc
      721 ccccgaagcc gccgccgaac ccaccgaggc cgccgccgcc gaacccaccg aggccgccgc
      781 caccaaaccc accgccaccc cagccaccgc cgccgcgacc accaaagccc ccaccgccaa
      841 agttgccgcc accgccccag cccccgaact ggccgtagcc ggcgccgcca actccgttga
      901 gcgcggccat ccggttgatg ttcgcgctgg cgaacttcga gccacgcatg ccctcgcggc
      961 ggagagcggc catctgggcc tcctgaagcg gccccagccc gcgtggtccc gtgctcacta
     1021 cggtgccacc caccgcggcg acaacagtcg gcaggtccgc atacttgacc tcgatgacct
     1081 cataatggcg aggctcctcg gccttctgct ctccgcccga cgcacccgcg ccaatggcgt
     1141 cggacgcaga gcggatgccc tcgaccgacc ctttgggctg gagcttcaga tcgggcgcgg
     1201 cgctgacgtt cagcacctcg gcagcccttc gcaactccgg ggcgtcagag gccgccaccc
     1261 cggtgcccgc tctctccgcc acggtccggc ccgcctcgga gtccttcgag tacacacaca
     1321 gtatgccgtt gcgggtttcg gaggccaggc gcaactccgg gttgcccaga atcgcctgaa
     1381 gcacctcgtc ggccgtggcg ctcgggatgc tcagatagcc aatctcgccc ttgacgtcgg
     1441 ccccaatcat gatgggcagc cggcgccact cggccacggc acgcaggacc tgcgacacgg
     1501 gcacatcctg gagttcgagc gagacgggct catcgccgag gccctcaaga tcggcgaggc
     1561 tggtcgccgt gtcgctggcg ccgactgcgg cggccgcatc cgcggctgcg cccaacgcct
     1621 cctcatgctc gagccggact gccgtgccgg tggtgtcgcc gggtaccgca agaaccgccg
     1681 atgcggccac gacgatgagt agtgtcgcga agccccagag cttaagcttc aacgctctga
     1741 cccctcccct agtccagcac gaggagcccg tgccgtgcct ctcacgaggg aacgacgccc
     1801 gtaacacaac ggtggccggg agattcccgt tcacccccgt cgagagacca atacatccgc
     1861 acatattcca gcagaggcgg cagcgcacct tccggcggct gccgcccgat ctcggcgccc
     1921 aaaacagggg atgctaccag ccgccgcccc atccgccgcc accacggttg ccgccccatc
     1981 cgccgccacc gcggttgccg ccccagctgt ttccgccacg gttcgacgag tagccgccac
     2041 cacggccacc gtagccaccg cctcggccgc catagccgcc accaccgtag ccgccaccac
     2101 cgtagccgcc gccatagccg ccgccaccgt agccgccgcc acccatctgc atcacgtcgg
     2161 tgttcatcag cacgtaaccg ccaccgaaga gaagggccgc gctgtagccg ccgatgtgct
     2221 tggcctcgat gacttcgtac aggtaatcct cggtctcctc ttcggaggct gttccgccct
     2281 gcccaccggc gcccgggccg gttccgccgc cggcgcccag accgaggccc ccggatcggc
     2341 cggtgcgtcc actcgacgtg ccgccgcgcc gattggtggt cccgccaccc atcatgccgc
     2401 tgccctgcgc gatctgcagc ggcgcgacgc ggtagacgat ctcggagtca tcaccggcaa
     2461 ggacgctctt gcccttgccc tcagaggcgc cctcagcacg cttgttcgtg gcacccgcct
     2521 tgttccggat cgttagcccc ccatcggcct cgaccttctc gagcccgtac ttgtccagga
     2581 tgaactgcag cgcgacggcc ggggtgacct cgctgagcag cacggtaacg tcctggtcac
     2641 ggacgtcctt ggcgaactcg atcttgggga gcccgaactt ggcgatggcc tgcacgactt
     2701 ccttgaggcc ctcgtcgtag cacttcactg tgatgaggtc gttgctggcc tgagcgaagg
     2761 cagttccgcc gcagaccagg agccccacgg ccaccagtgt gagacccggg agaaagcgtc
     2821 gcatccctac accactcctc ttctcgcctc gcgccgtccg gccgaaccgc acgccgcata
     2881 cggcgtggaa gcaccgcgcg atgcccccaa gagacggtcg agctgccctc gggttcgcgg
     2941 cagcagtacc gtcaaccttc gttggcggtt acgccccgca ggcgcacgac aacatcctcc
     3001 gtcgacccgg tctctcggag cgacacggag tctgcgccaa tggcacgcac cgtaaactgg
     3061 cgaccgccgg cgcggattcg gtcgcccggc ttgacaattc gccctaccgg cttgtcgccc
     3121 tcgtacacct cgatgatcgc gagcgaaaca ccatcatgaa ggataccgga caccctgcgg
     3181 aacgaccagt ctggcggttt gcgggtcacg cgcggcccca cggcccccac gctttgcgct
     3241 ccacccgcaa cgacgcgcgg agcttcttct tgcgatgtgc ccacaaccgg acggacggac
     3301 tcgccaccga gcttcggcgg ttgggcgcgc gggatggttg ggttcgggac aaaccatcca
     3361 gccggctgga tgccgatggg ctcggggccc gcaagcacgg gctggcgaac cttccgtggc
     3421 cgggggaatc cgcgctcggc gaagacatcg gccggatcgt gcattggcag agatgcactc
     3481 aggctagaac gcttggaggc gaaggggttc tccttgaacg ggaacagtgg gtcaccgaag
     3541 tccgtgacgg ccaactggcc ctgcatcgtt gccgcgccct c
//
LOCUS       DAOSPL010000299         3576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26180_length_3576_cov_3.009940, whole genome shotgun sequence.
ACCESSION   DAOSPL010000299 DAOSPL010000000
VERSION     DAOSPL010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26180_length_3576_cov_3.009940"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PLQ54_06805"
     CDS             <1..837
                     /locus_tag="PLQ54_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93006.1"
                     /translation="RVACRRGRALGFAAVWVAAAMVSAGWADPVTLKYAPEKGAKRTY
                     QVTMQGTVDVSEYAAGVGAEGRAVYTEEVTESEAGKVVSSVVIDKGHLSAAGAYLPST
                     SADETLVCTRDATRRVVAVEGTAVAYAGEDPVVLCLWALDGFLLPKDPVEPGATWDCS
                     HTTIGPDGTPRKVTGEATLVEVKDVEGTPVAVVRSTLTTPVSYAYGDPMTGDIRAEVT
                     SELVLESGEIRAQDGVMSGTIATGPEAFQKLSVTFNDVQVTVRRVEPKAEGQPVTAPQ
                     PN"
     gene            863..3115
                     /locus_tag="PLQ54_06810"
     CDS             863..3115
                     /locus_tag="PLQ54_06810"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOS93007.1"
                     /translation="MEASPPNRLRRWIPYAAFLVLAIAACPGVIVGRALVPAHYQQHW
                     APWGDPPVATAGEPIWNPLAADGVLQFYPWRHFARVTLRSGEVPLWNPYQFCGTPFAA
                     NGQSAVFYPPNLLFWLLPTASAFGWSAVVHLFMAGTFTYWLGRAVELSDAAASLAGVA
                     FMLCGWLIAWLPLPTFMNVIVWLPLAMCAGKRHFDGTGGLAWPLVMGSSIACMVLAGH
                     FQIAYYALVTLVAWLIALKGVTWRRLGFRATLGLEVQEKSGFVLGLLAGAIQLLPSAE
                     LVHYGHRIGAPPFASLAEGALGRYDLAALVAPNLLGNPSHGWYLTPLTQNRMEMCGYV
                     GTMALVLAGAGLAACRRNWAARFFAALAVGALLVAAGTPLAHTLYVGLPGFNRFSGLP
                     RVLCLFCLGAALLAGYGVDELRRAREGRDWALAGSVAVAVLGLLCLARVGPEAVATDR
                     RAALPVAVGGLGLVLTLLLVRFGGTRRLGWMWVCLAGVELLGLGRGTNLTIEPSNIYA
                     ETALTNELRQLPVSQRVLALTDDWTFGRPPLTVLPPNSATVYGIRDVQGYDSLFAAHT
                     KSYLMRANGGRQPCPAINGNMVLLGRNGPDPVAAIDPKLLRQMGVTRVLSRRPLPSGW
                     GARHLGERGHGVSLYELPSGRDPGPMVTGLAANSVTLAGTMTGEGMWPTRESYYPGWY
                     SVGPDGQQPCGMSDRMFRTVQPGATRAIYLPTTYWVGAFVSLVALAGLCALASWAYAG
                     ARQSRAGGDG"
     gene            3115..>3576
                     /locus_tag="PLQ54_06815"
     CDS             3115..>3576
                     /locus_tag="PLQ54_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93008.1"
                     /translation="MRRASGPGPGVAAVAVLVFVPALLLARSLFTPRILLPADVLLLM
                     EPWRSEARGLVPGFQRPQNPMLDPIQQQLPWRTHAARELRQGTVPLWNPYMFSGTAFV
                     ANLQSSIYYPPNLLFCVLPVPQAFEWVAYLHLVMAGLFAFGFARGLGLGHWP"
BASE COUNT          462 a         1199 c         1263 g          652 t
ORIGIN      
        1 cgtgttgcat gccgcagggg gcgggctctc gggttcgccg cagtgtgggt tgccgccgcg
       61 atggtttctg cgggttgggc tgatccggtg accctcaagt acgccccgga gaaaggagcc
      121 aagcgcacct accaagtgac catgcaaggc acggtggatg tgtcagagta tgccgcgggc
      181 gtaggggccg aggggcgcgc cgtgtatacc gaggaggtta ccgagtccga ggccggcaag
      241 gtggtcagca gtgtggtgat cgacaagggc cacctcagtg cggctggcgc ttacctgcca
      301 tcgacctcgg cggatgagac gctggtgtgc acgcgggacg cgactcgccg ggtggtggcc
      361 gtggagggaa ccgccgtggc ctacgcgggc gaggatccgg tcgtgctgtg cctctgggcg
      421 ctggatggct tcctgctgcc caaggacccg gtcgagcccg gcgccacatg ggactgcagt
      481 cacaccacca tcgggcccga cggcacgccg cggaaggtca cgggggaggc caccctggtc
      541 gaggtgaagg acgtcgaggg cacaccagtc gccgtcgttc gctcgacgct gaccacaccg
      601 gtctcgtacg catatggtga ccctatgacg ggagacatcc gcgccgaggt aacctcggag
      661 ctcgttctgg agtcgggcga gatccgggcg caggatggtg tgatgagcgg taccatcgcc
      721 acgggccccg aggccttcca gaagctcagt gtgacgttca acgacgttca ggtcacggtt
      781 cgccgggtcg agcccaaggc cgagggccaa cccgtcacgg cgcctcagcc gaactaggtt
      841 gcgacggggc tgacgagaga ctttggaggc ctccccgcca aaccggctcc gccgctggat
      901 tccctacgcg gcgttcctgg tcctggccat cgcggcgtgc cccggcgtga tcgtggggcg
      961 cgccttggtt ccggctcact accagcagca ttgggctccg tggggcgatc cgccagtggc
     1021 cacggccggc gagcccatct ggaacccgtt ggccgcggat ggggtacttc agttctaccc
     1081 ctggcggcac ttcgcgcgcg tgacgctccg gtcgggcgag gtgccgctgt ggaacccgta
     1141 ccagttctgc ggcacgccct tcgccgccaa tgggcagtcc gccgtcttct acccgccgaa
     1201 cctcctcttc tggctcctgc ccacggcctc ggccttcggg tggtccgccg tggtgcatct
     1261 cttcatggcc ggcacgttca cctactggct gggccgggct gtggagctca gcgatgccgc
     1321 cgcgtccctg gctggcgtgg cgttcatgtt gtgcggctgg ctgatcgcct ggctgccgct
     1381 gccgacgttc atgaatgtca ttgtgtggct cccgctggcc atgtgcgccg ggaagaggca
     1441 cttcgacggc accggagggt tggcgtggcc gctggtgatg gggtcttcga tcgcctgcat
     1501 ggtgctcgcc ggccacttcc agatcgccta ctacgccctg gtcaccctgg tggcgtggct
     1561 catcgcgctg aagggggtca catggcgccg cctggggttc cgggcgactc tggggctgga
     1621 ggttcaggaa aagtcgggct tcgtgctggg gctcctggcc ggggccatcc agctgttgcc
     1681 ttcggccgag ctggtccact acggccaccg catcggagca ccgccgtttg cctcgttggc
     1741 cgaaggggcg ctaggccgat acgacctggc ggcgctggtg gccccgaatc tgctcgggaa
     1801 cccgtcccat gggtggtacc tcacgccgct gacgcagaac cggatggaga tgtgcggcta
     1861 cgtgggcacc atggctctcg tgctggccgg tgccggcctc gcggcatgcc ggcgcaactg
     1921 ggcggcgcgc ttcttcgccg ccctggcggt cggcgccctg ctggtcgccg cgggcacgcc
     1981 gctcgcccat acgctgtatg tggggctgcc cgggttcaac cgcttctcgg ggctgcctcg
     2041 ggtactgtgt ctgttctgcc tcggggcagc gctgctcgcg gggtacggag tcgatgaact
     2101 caggcgggcc cgcgagggcc gcgactgggc cctggccggg agtgtcgccg tcgcggttct
     2161 gggcctcttg tgcctggccc gtgtgggccc cgaggctgtc gccacggatc gccgggccgc
     2221 gcttcctgtg gccgtgggcg gcctcgggct cgttctcacg ctcctgttgg tccgattcgg
     2281 cggcacccgt cggctcgggt ggatgtgggt gtgcctcgct ggagtcgaac tgcttgggct
     2341 ggggcgcggc actaacctga ccatcgaacc gtcgaacatc tacgccgaga cggcgctcac
     2401 gaacgagctg cgccaactgc ccgtgtccca gcgcgttctg gcgctgaccg atgactggac
     2461 cttcggccgg ccgccactca cagtgctgcc gcccaacagc gcgacagtgt atgggattcg
     2521 cgacgtgcag ggctacgact cgttgtttgc ggcccacacg aagagctatc tcatgcgggc
     2581 gaacggaggg cggcagccct gcccggccat caatgggaac atggtgctcc tggggcggaa
     2641 tggcccggat cccgtggcgg ccatcgaccc caagctactg cggcagatgg gcgtgacccg
     2701 cgtcctgagc cggcgtccac ttccctcggg ctggggggcg cgccacctcg gcgagcgcgg
     2761 gcacggcgtc tctttgtatg agctgccttc cggacgcgat ccgggaccca tggtgaccgg
     2821 tctggccgcg aactccgtga ctcttgccgg cacgatgacc ggcgagggta tgtggccgac
     2881 gcgggaaagc tactacccgg ggtggtactc ggttgggcca gacggccagc agccgtgtgg
     2941 gatgagcgac cggatgtttc ggaccgtaca gcccggcgcc acccgggcga tctacctgcc
     3001 caccacatac tgggtgggcg ccttcgtctc gctggtggct ctcgccggcc tctgtgccct
     3061 ggcctcgtgg gcgtatgccg gcgccaggca gtcccgggcg gggggcgatg gctgatgcga
     3121 cgtgcttccg gccccgggcc aggcgtcgcg gccgtcgctg ttctggtctt cgtgccggcg
     3181 ctgttgctcg cacggtcgct gttcactcct cgcatcctgc tccccgccga cgtgctgctg
     3241 ctcatggaac cgtggcgctc ggaggcgcgc ggactggtcc ccggcttcca gcggccccag
     3301 aacccaatgc tcgaccctat ccagcagcag ctcccatggc gcacacacgc cgcccgcgaa
     3361 ctccgccagg gcaccgtccc cctgtggaac ccgtacatgt tctcgggcac cgcctttgtc
     3421 gcgaacctgc aatcatcaat ctactacccg ccgaatctgc tcttttgcgt gcttcccgtg
     3481 ccccaggcat tcgagtgggt ggcgtaccta cacctggtga tggccgggct gttcgcattc
     3541 gggttcgccc gaggtctcgg gctcgggcac tggccg
//
LOCUS       DAOSPL010000300         3576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26181_length_3576_cov_2.980119, whole genome shotgun sequence.
ACCESSION   DAOSPL010000300 DAOSPL010000000
VERSION     DAOSPL010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26181_length_3576_cov_2.980119"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PLQ54_06820"
     CDS             <1..415
                     /locus_tag="PLQ54_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93009.1"
                     /translation="SLVVIDEGAPEPWVGHLKYYLNADRTAFREWALSQPIAPGQLTQ
                     AKLERLMMRYAGRPYREVRAVPEGRLAPMTLTTLDFPEAERADVLRSLRTYAKDAACA
                     ERLVALYRKLPKRLRVLGERIGSVQAMRQAIFGAE"
     gene            complement(462..1541)
                     /locus_tag="PLQ54_06825"
     CDS             complement(462..1541)
                     /locus_tag="PLQ54_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOS93010.1"
                     /translation="MAMPPNVLLQGLATAKPFIAKRKSSWDVTGGNWDAWRIEPGETK
                     VLADIDGPGVISHIWFTISSPDALHLRKLLLRAYWDGEDNPSIDCPVGDFFGLGHART
                     YSYQCALFNTSCHQDGGVGGGCAMNSWVPMPFHRNARIEIVNEQDDPVHSFYFYIDHQ
                     LYPELPDDALWFHAKWRRDNPCEGWKGKGSVWFSPEWHDRQNGPEGKNLSDKGNYLIL
                     EAEGRGHYIGANFSIDHLYKGWYGEGDDMIFVDGEPWPPSLHGTGTEDYLGHAWGMQK
                     NAHLYNGQAWAEMDNFNEWGKVCVYRYHVVDPVPFTKSIRVSIEHGHANDRSDDYSSV
                     AYWYQTEPHRDFAPMPPVKARIPNG"
     gene            1709..2971
                     /locus_tag="PLQ54_06830"
     CDS             1709..2971
                     /locus_tag="PLQ54_06830"
                     /inference="COORDINATES: protein motif:HMM:NF015616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 76 protein"
                     /protein_id="HOS93011.1"
                     /translation="MLAAVGPDLVTAGPDDAARYLEAARRLTDTFQSTFWDPAARMYR
                     APQRSAETVDSDPQHNNGYVLWPSVEALHALVEANRSLPGRYRAALREVFGGLERYFD
                     AERKAYNAWLNFPGNNDTYYDDNAIVVIALTKAFRVTRAKAYIDRAWDVMSRFCLSGW
                     DDGEPGGVRWGTDPTRAGTADRAACSTGLAALAALLLADTGVAVESNIEWGRRALQWL
                     VEHLLDTDGLVMDALEPPDWRLRRVKWTYNTGIAICAHVLLYRLATDTRALGIARRLA
                     DATLHRGGALYDSLVHDMEHRFLYDSSYFVPWVIEGWLALYRETRDPLLLREAQRHAD
                     FALDYVRDPADGLFFRNWRLWRISEEQLRMWERLTGQEGHLDPDEDERSKASGHLALP
                     VAQRPLVKTLLANAGCARLLWLVGRASR"
     gene            complement(2976..>3576)
                     /locus_tag="PLQ54_06835"
     CDS             complement(2976..>3576)
                     /locus_tag="PLQ54_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93012.1"
                     /translation="VEESAESLYDESLMGGLYIGGLVGALAGYFQAKGSGGSLWGGPI
                     VGAVIGALIGLAIGVAVGRGGAAGVRTHRGQAFLIGVGIVLLAGPIIAVLVALMAWGF
                     GKHPLWAALSYLVLAFAFFALTWQDAKRRQADSPLTWGLIVAALPPLLGLYYSRRPHG
                     ELVQCGSPQCGEWHLSTHTKCPHCGYARRAGVIEKHVEL"
BASE COUNT          582 a         1223 c         1159 g          612 t
ORIGIN      
        1 cagcctggtc gtcatcgatg agggggcgcc ggagccctgg gtcggtcatc tcaagtacta
       61 tctgaatgcc gatcgcacgg ccttccggga gtgggccctg agtcagccca tcgcccccgg
      121 ccagctcacg caggccaagc tcgagcggct catgatgcgc tacgccggcc ggccttaccg
      181 ggaggtccgg gccgttccgg agggcaggct tgcgccgatg acgctcacca cgctggactt
      241 ccccgaggcc gagagggccg acgtgctccg ctcgctgcga acctacgcca aagacgccgc
      301 gtgcgccgag cgcctcgtgg cgctgtaccg caagctgccc aagcggctgc gggtcctcgg
      361 agagcgcatc ggcagtgtcc aggcgatgcg gcaggccatc ttcggggcgg agtaggtctg
      421 acgccagagg ccgccgggcg gcctctggcg tcgttgctcc tctacccgtt gggtatgcgc
      481 gccttcaccg gcggcatggg tgcgaagtcc cggtgcggtt cggtctggta ccagtaggct
      541 actgagctgt agtcgtcgct ccggtcattg gcgtggccgt gttcgatgga tacccggatg
      601 ctcttcgtga agggtacggg atcgaccacg tggtaccggt acacacagac cttcccccac
      661 tcgttgaagt tgtccatctc cgcccaggcc tgcccgttgt agaggtgagc gttcttctgc
      721 atcccccagg cgtgacccag gtagtcctcg gtgcccgtcc cgtgtagcga tggcggccag
      781 ggctcaccat cgacgaagat catgtcatcg ccctcgccat accagccctt gtagaggtgg
      841 tcgatgctga agttggcacc aatgtagtgc cctcgccctt cggcctcgag gatcaggtag
      901 ttgcctttgt ccgagaggtt cttcccctcc gggccgttct gccggtcgtg ccactcgggc
      961 gagaaccaca cgctgccctt gcccttccac ccctcgcacg ggttgtcgcg ccgccacttg
     1021 gcatggaacc agagggcatc gtcgggcaac tctggataga gctgatggtc tatgtagaag
     1081 tagaagctgt gcactggatc gtcttgctcg ttgacgattt cgatgcgggc gttgcggtgg
     1141 aagggcatgg gcacccacga gttcatggcg cacccgccac ccactccgcc atcctggtgg
     1201 cagcttgtgt tgaacagggc gcactggtag ctgtacgtcc gggcgtggcc caggccgaag
     1261 aagtcgccca ccgggcagtc gatggaaggg ttgtcctcgc catcccagta ggcgcgcaga
     1321 agcagcttgc ggaggtgcaa tgcgtcgggc gatgagatgg taaaccagat gtgcgaaatg
     1381 acaccagggc cgtcgatgtc ggccaggact ttggtctcgc cgggctcgat acgccaggca
     1441 tcccagttcc cgcccgtgac atcccacgag ctcttgcgct tggcgatgaa tggtttggcc
     1501 gtggccaggc cctgcagcag cacgttcggt ggcattgcca tgtggggacg tccctccgga
     1561 gcgatgtcgg attgggcggt ctaccagagc cgccaggcgc ccagatggtt ggcgccctga
     1621 gcttggaaga ccttctcggc acagggagtg gttgcccatg gtatggccca gcaggctgag
     1681 ccgacgcgag ttcctcagga ggggcgccgt gctcgcggcc gtcggccccg acctcgtcac
     1741 cgcgggtccc gacgatgccg cgcggtatct ggaggccgcc cgacgactga ctgacacctt
     1801 tcagagcacg ttctgggatc ctgcggcgcg catgtaccgg gcgccccagc ggagcgccga
     1861 gacggttgac agtgatcccc agcacaacaa cggctatgtg ctctggccct ccgtcgaagc
     1921 ccttcacgcc ctcgtcgaag ccaaccgtag cctgcccggc cgctaccgag ccgcccttcg
     1981 cgaggtcttc ggcgggctcg agcggtactt cgacgcggag cgcaaggcct acaatgcctg
     2041 gctcaacttc ccggggaaca acgacacgta ctacgacgac aatgccatcg tggtcatcgc
     2101 gctgaccaag gcgtttcggg tgacacgcgc caaggcctac atcgaccggg catgggacgt
     2161 aatgtcgagg ttctgcctga gcggctggga cgatggtgag cccggtggcg tacggtgggg
     2221 caccgacccg acccgagccg gcaccgcgga ccgcgcggcc tgctccacgg gcctcgccgc
     2281 actagcggcg ctgctgcttg ccgacaccgg cgtagcggtc gagtcgaaca tcgagtgggg
     2341 ccggcgggct ctgcagtggc tggtcgaaca cctgctcgac actgacggcc tggtgatgga
     2401 tgctctcgaa ccgcccgact ggcggctgcg ccgggtgaaa tggacctaca acaccggtat
     2461 cgccatctgc gctcacgtcc tgctctaccg gctggccacc gatacccgcg ctctcggcat
     2521 cgcccgtcgt cttgccgatg ccaccctcca ccgtgggggc gcgctctacg acagcctggt
     2581 gcacgacatg gagcatcggt tcctctatga ctcgagctac ttcgtgcctt gggtgattga
     2641 gggatggctg gccctctacc gcgagacacg cgacccacta ctgctccgcg aggcgcagcg
     2701 ccacgcggac ttcgcgctgg actacgtgcg cgacccggcc gatggcctgt tcttccgcaa
     2761 ctggcggctc tggcgcatca gcgaggagca gctccgcatg tgggagcggc tcacggggca
     2821 agagggccat ctcgaccccg atgaggacga gcggagcaaa gcttccgggc acctcgcgct
     2881 ccccgtggcc cagcggccgc tggtgaagac actcctcgcc aacgccgggt gcgcacgcct
     2941 gctctggctc gtggggaggg cgagtcggta ggcctctaca gctccacatg tttctcgatg
     3001 acgccggcgc ggcgcgcata gccacagtgc gggcacttgg tgtgcgtcga gagatgccac
     3061 tcgccacact ggggagaccc acactggacc agctccccat ggggcctgcg agagtagtac
     3121 agacccagca acggcggcag cgcggccacg atcagccccc aggtcaaggg gctgtcggcc
     3181 tgcctgcgtt tcgcatcctg ccaggtcagc gcgaagaaag cgaaagccag gaccaggtat
     3241 gacagtgccg cccacagggg atgcttcccg aatccccagg ccatcagcgc caccagcacc
     3301 gcgatgatgg gcccggccag cagcacgatg cccacaccga tcaggaacgc ttgtccccga
     3361 tgggtccgca cccccgccgc tcccccgcgc cctacggcga cgccgatcgc cagaccgatc
     3421 agggcgccga tcaccgctcc cacgatcggc ccgccccaga gactcccccc gcttcccttc
     3481 gcctggaagt agcccgccag tgcgcccacc agcccgccga tgtacagtcc ccccatgagg
     3541 ctctcgtcat atagcgactc ggcggactcc tcgacg
//
LOCUS       DAOSPL010000301         3572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26218_length_3572_cov_4.076770, whole genome shotgun sequence.
ACCESSION   DAOSPL010000301 DAOSPL010000000
VERSION     DAOSPL010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26218_length_3572_cov_4.076770"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..958)
                     /gene="prmC"
                     /locus_tag="PLQ54_06840"
     CDS             complement(92..958)
                     /gene="prmC"
                     /locus_tag="PLQ54_06840"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOS93013.1"
                     /translation="MITSTDGLSARELHRALTSLLTGSPYVTAPGSEAAWIVGHLSGR
                     SWAQLLAGTGTPLPPDVSAQAEAIALRRRAGEPLQYLLGDAYFYGHRFLADRRVLIPR
                     PETELLVSVCAERLRDRRAPVFADIATGSGCIAISVVLELPHARGVATDLSASALEVA
                     LKNARLHGVEDRVEFRQGHLLEPLKGLLFDALLINPPYIPSGDIASLGPELAAEPVMA
                     LDGGPDGLDFYRAIARHWRDALFPGGFMACEVGAGQMPEVLRLMGPGRYRVVPDLSGV
                     ARALVAERGAVR"
     gene            complement(955..2016)
                     /gene="prfA"
                     /locus_tag="PLQ54_06845"
     CDS             complement(955..2016)
                     /gene="prfA"
                     /locus_tag="PLQ54_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOS93014.1"
                     /translation="MLDRLLTYRTRYEEIGARLADEAVLSDPSAYRALSKEYSDLEPL
                     ASMHREAERLREELEGARELTADPELREEAELEAQRLVQALAELEERAARLLLADNDP
                     DSDRNAIIEVRAGTGGEEAALFARDLYRMYSRYAERKGWKTELIDLNETGIGGIKEVV
                     FSLQGRGAYGQMQFESGVHRVQRVPETESSGRIHTSAATVAILPEAEEVDIEIDPADL
                     EVDTYRSSSAGGQHVNKTDSAVRITHVPTGIVVQCQDERSQRQNRVKAMRVLRAKLLD
                     KQRQEQEAAIASSRKSQVRTGDRSEKIRTYNFPQSRITDHRIGYSTYNLEAFLDGDIE
                     DMIERLRAAAAEEALAARE"
     gene            complement(2006..3310)
                     /locus_tag="PLQ54_06850"
     CDS             complement(2006..3310)
                     /locus_tag="PLQ54_06850"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOS93015.1"
                     /translation="MLYRHVAAEAVAEVARRDAVEEPSARGAAELCGFRSDKPVYGGQ
                     AVIEGVMMRGKRFAATSVRREDGEIIVHTEEISGFVSRHPWINRPLLRGVFTLFDSLL
                     AGYRSLRFSADVLTEEMRQQESSEFDASAVREELFGDAGVRMVHPETGLSVESAREAD
                     HERNRARALRLLRGKVARHRALGGAREPGPSAPGGLAIEPATPERPVEQNAASGMPGW
                     STAVMFLGLAFAVIFVVVVPHLIGKYLSTRAGLEAYAYQAGPVRVNLGSNIIEGLIRL
                     GLFLGYVWVIGFMPDIRRVFQYHGAEHMTVYGVEAGVELTPANVRRFSRLHPRCGTNF
                     IFIVICVKIVLFSFLEWDNPTLRIVGRLALLPVVAAVSYEILRLAGRHRDSRVTRALI
                     APGLWVQRLTTREPDDSQIEVAILSMRRVLDLELAAREPHAG"
BASE COUNT          644 a         1346 c         1018 g          564 t
ORIGIN      
        1 cgcgcgtagg accatgcgcg gcggaacgac gacgcgcgtt tctggcccgt ctccatcccg
       61 aacgttccac ttcccccgcc gtggtcaggc cctagcgcac cgctccccgc tccgccacca
      121 gcgccctcgc cacacccgat aggtcgggga caacccgata ccgccccgga cccatcaacc
      181 tcaagacctc cggcatctgc cccgccccaa cctcgcacgc catgaagcca cccggaaaca
      241 gagcatctcg ccaatggcgc gcgatggcac ggtagaaatc gaggccgtcc ggaccgccat
      301 cgagcgccat caccggctcg gcggccagtt cgggacccaa ggaggcgatg tcgccagagg
      361 ggatgtacgg tgggttaatg agcagcgcat cgaacagcag gcccttcagt ggctcgagca
      421 gatgcccctg ccggaactcc acacggtctt cgacgccatg caacctggca ttcttcaggg
      481 cgacctccag ggcgctggcc gacaggtcgg tggccacgcc tcgggcatgg ggcagctcca
      541 gcaccaccga aatggcaatg catccgctgc ccgtcgcgat gtcggcgaac accggcgctc
      601 ggcgatccct cagccgctcg gcgcacacgc tcaccaacaa ctccgtctcc ggccggggga
      661 tcagcacgcg acggtcggcg aggaaacggt gcccatagaa gtacgcatcg cccagcagat
      721 actgcagcgg ctcgcccgca cgccggcgca gagcaatggc ctcggcctgc gccgaaacgt
      781 cgggcggcaa cggcgtccct gtgcccgcga ggagctgagc ccagctccgg ccgctcagat
      841 ggcccacaat ccatgccgct tcggagcccg gcgccgtcac ataagggcta ccagtcagga
      901 gggatgtcag cgcgcgatgc agctcccggg ccgacaggcc gtctgtcgag gtgatcactc
      961 cctggcggcc aaggcctcct cggccgcggc cgcacgcaac cgctcaatca tgtcctcgat
     1021 gtcgccatcg aggaatgcct caagattgta agttgagtag ccaatgcggt ggtctgtgat
     1081 ccggctctgc ggaaagttgt acgtgcggat cttttcgctc cgatcaccgg tgcgaacctg
     1141 ggacttccgt gacgatgcga tcgccgcctc ctgctcctgc cgctgcttgt ccagcaactt
     1201 ggctcgcagg acccgcatgg ccttgacccg attctgacgc tggctccgct catcctgaca
     1261 ctgcaccacg atgccggtag gcacgtgggt gatgcgcaca gcggaatccg tcttgttcac
     1321 atgctgccca ccggcactgc tcgatcgata agtgtcgacc tccagatccg ccggatcgat
     1381 ctcaatgtcg acctcctcgg cctcggggag gatggctacg gttgccgccg acgtgtggat
     1441 ccgcccgctc gactccgtct cgggtacccg ctgcacacga tgcacgccac tctcaaactg
     1501 catctgtcca tacgcgcccc gtccctgtag cgagaacacg acctccttga tgccgccgat
     1561 ccccgtctca ttcaggtcga tcagctcggt cttccacccc ttgcgctcgg catagcggct
     1621 gtacatccgg tacaggtcac gcgcgaacag cgccgcttcc tcaccgcccg ttcccgcgcg
     1681 cacctcgatg atcgcgttac ggtccgagtc ggggtcgttg tcggccagca acagccgggc
     1741 ggcccgctcc tcgagctcgg ccagtgcctg taccagccgt tgcgcctcaa gctccgcttc
     1801 ctccctcagt tcaggatccg cggtgagctc ccgggcaccc tcgagctcct cccgcagccg
     1861 ctctgcctcc cggtgcatcg acgccagcgg ctccaggtcc gagtactcct tcgacagcgc
     1921 gcgatacgcc gacgggtcgg agagcaccgc ctcatcggcc aaccgcgcgc cgatctcctc
     1981 ataccgtgtt ctgtacgtca gcagtctatc cagcatgggg ctccctcgcc gccagctcga
     2041 ggtcgagcac gcgcctcatc gacaggatcg caacctcgat ctggctgtcg tccggttcgc
     2101 gcgtggtcag ccgctgcacc cacagaccgg gcgcaatcag cgcgcgcgtc acgcggctgt
     2161 cccggtgccg tccggccagc cgcagaatct cataggacac cgccgccacc acggggagca
     2221 acgccaggcg ccccacaatc cgcagcgtcg ggttgtccca ctccagaaac gagaacaaca
     2281 cgatcttcac gcagatcacg atgaagatga agttcgtccc acaccgagga tgcagtcgcg
     2341 agaaccgccg aacattcgcc ggcgtcagct caacaccggc ctcgactcca tacacggtca
     2401 tgtgctccgc cccatggtac tggaacacac ggcggatgtc gggcatgaac ccgatcaccc
     2461 acacgtagcc aaggaacaga ccgagtcgaa tcagtccctc aatgatgttc gaccccaagt
     2521 tcacccgcac cggccccgcc tgataggcat aggcctcgag acccgcccgg gtcgacagat
     2581 acttcccgat gagatgtggc acaaccacaa cgaagatcac ggcgaacgcc agcccgagga
     2641 acatgacggc cgtcgaccat cccggcatcc ccgacgcggc gttctgctcc accggccgct
     2701 cgggagtggc gggctcgatt gcgagacccc ccggggccga gggtccgggc tctctggcgc
     2761 cccccagcgc ccgatggcga gccaccttgc ccctgagcaa ccgcaatgcc cgcgcgcggt
     2821 tgcgttcatg atcggcctca cgcgccgact cgacgctcag acccgtctcg ggatgcacca
     2881 tccgaacgcc ggcatcgcca aaaagctcct cgcgaaccgc cgaggcatcg aactccgagc
     2941 tctcttgctg ccgcatctcc tcggtcagca catcggccga gaagcgcaac gagcggtagc
     3001 cggccaggag cgagtcgaac agcgtgaaca ctccccgcaa caggggccgg ttgatccacg
     3061 ggtgacgcga tacgaaaccg gagatctcct cggtgtggac gatgatctct ccatcctcac
     3121 gccgcaccga tgtcgcggcg aagcgcttcc cgcgcatcat gacgccctcg atcaccgcct
     3181 gcccaccgta gacaggcttg tcgctccgga aaccacacag ttcggccgcg cctcgtgcgc
     3241 tgggctcctc taccgcatca cggcgagcca cctcggccac tgcctctgct gcaacatgac
     3301 gatacagcaa gtcataacct cgcttcgtct cgccgtcccc cggctccaca aacccacgcc
     3361 gggccagtgt ctcagtatac ctcgaactgc ccgggccagc ccaagcaggg cattggcggt
     3421 gactcagcgc tccagcggcc catggaacgc ctcgccgagc ggcgccatag ggcgaacctc
     3481 gcgctccgcg cgccgcccgg gcagggaccc ttcccctctg tggcgcatta ccaatcgagt
     3541 ctcgcctgtc tcctgccgca gaccaccatc gg
//
LOCUS       DAOSPL010000302         3569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26254_length_3569_cov_2.348606, whole genome shotgun sequence.
ACCESSION   DAOSPL010000302 DAOSPL010000000
VERSION     DAOSPL010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26254_length_3569_cov_2.348606"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PLQ54_06855"
     CDS             complement(<1..396)
                     /locus_tag="PLQ54_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93016.1"
                     /translation="MSATTSQTHNGPRTYGNWTRPKSPGLLGLGAIGTGVLFVGAGIT
                     IVVSIIGGLLAGFVVAVVTIGFLLLIALRDKHGQSTLARTATRFGWANTRARRKNIYR
                     SGPLGRADWGTMQLPGLAAASRLVEYKDSY"
     gene            complement(405..1952)
                     /locus_tag="PLQ54_06860"
     CDS             complement(405..1952)
                     /locus_tag="PLQ54_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93017.1"
                     /translation="MACDPIGDPFGCITDGISGIVSAAANNTLQELTNQALQGFGQAV
                     GSLGTMWVYLPSPVLTNGTGQAGGGTPPGSAGVTTILGYVTWIALVICVLSIIAIGAM
                     LAVRIRRGDGEQLLGRLGIVLAAVILISGGTGLVAGLLPNQAPGGSASAVGFLQNSLW
                     WYVGAMAVVSVIIAGIRMAWEQRANPGKELLQSLLTLIVVSGAGLTIIGLAVAATDAF
                     SVWVLNGATQCDVSAAGESNCFGTNVAAMIGLSSTSPLALLAILILALLAMLMSWVQV
                     ALMIVRGGLLVILAGVLPITASFTNTQMGRQWFQKAIGWTLAFILYKPAAALIYAAAF
                     RLTGSDAFGANGVGIVSILTGMGLMILALFALPALLRFVAPMTSAAIGGGSGGLAMGA
                     MGAAMGVEAATGAISRTSSSSVTAGASSAAPSGASAGGGSAAGAGGGAAVGGGAAGGG
                     AAAGGGAAAGGAAAGGAAAASAATGVGIPVAAGIAVAQGASQVGGAIKSGVQSAAADS
                     AGEPSGA"
     gene            complement(1954..2679)
                     /locus_tag="PLQ54_06865"
     CDS             complement(1954..2679)
                     /locus_tag="PLQ54_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93018.1"
                     /translation="MSDDSTQPSNPFASRGFIAAAIVIGVILLAGVVVLVTALTAPHT
                     PVAQPTSTPSGPVASGDDKSVCGLPGFETESSLTAAPATKWELVGTVAAPSDPKVGPG
                     KVSSDGIRSCYAHTAEGALYAIVNYMALASDARTLPHLPELVEPGKGRDAAVAATSPD
                     AAPSNSRLQVAGFKVNSYSCDEAVIDVAWTVTSSGNQLVSLPLVMHWVDGDWKVSLTD
                     DGKFPFASAPLESLGGYIPWAGV"
     gene            complement(2700..2996)
                     /locus_tag="PLQ54_06870"
     CDS             complement(2700..2996)
                     /locus_tag="PLQ54_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017607246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93019.1"
                     /translation="MSLLAHSFDLINSLPADIPDPGPVAPPGFAGPVAVIMGWVKWVG
                     LIVAVLGVIIIGGKLAINVRRGEAAGELGGLLYVGLGCILIGAAASIVGFISGS"
     gene            complement(3017..>3569)
                     /locus_tag="PLQ54_06875"
     CDS             complement(3017..>3569)
                     /locus_tag="PLQ54_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93020.1"
                     /translation="IVEPPGTTGPKPASVTAPQDGVPVPDQADRLPQRKTPHGATVWW
                     LGVTGGAGESTLASLARGSRSADHAWPIPENRGSLCRVVLVARTNYAGLAAAQRAAIE
                     WASGVLGDAVRVDGLVLIPDMPGRMPKELRHLAQVVSGGLPHTWSLPWVDAWRFGPLD
                     PAADLPKEFHALLADLHLNSTAP"
BASE COUNT          632 a         1244 c         1194 g          499 t
ORIGIN      
        1 gtacgagtcc ttgtactcga cgaggcggga tgccgcggca aggcccggca gctgcatggt
       61 gccccagtcg gcacgaccca gcgggcccga gcggtagatg ttcttccggc gtgcccgcgt
      121 gttcgcccag ccgaaccggg tggctgtgcg ggcgagcgtg ctctgcccgt gcttgtcgcg
      181 caacgcgatc agcagcagga agccgatcgt gacgacagcc acgacgaagc cggccagcag
      241 cccgccgatg atcgagacga cgatcgtaat gccggcgccg acgaagagaa cgccggtgcc
      301 gatcgcaccg aggcccagca ggcccggcga cttcggccgc gtccagttgc cgtaggttcg
      361 tggcccgttg tgtgtctggc tcgtggtggc gctcatgact ccccttatgc tccgcttggt
      421 tcaccggctg agtccgcggc tgcggactga acaccggact tgatagctcc gccgacctgc
      481 gaggcaccct gcgctacagc gatgccagcc gcgaccggga tcccgacacc cgtagcggcg
      541 gatgccgcag ctgcaccccc ggcagctgca cccccagccg cagcgccacc gcccgcagcg
      601 gccccgccgc ctgcagcacc accgccaacc gcggcgcccc caccggcgcc ggcagctgaa
      661 ccgcccccgg ccgaggctcc ggacggggca gctgaggatg caccggcggt aaccgacgac
      721 gagctggtgc gactgatcgc gcccgtcgcg gcctcgacgc ccatcgccgc gcccatggca
      781 cccatggcca gcccgccgct gccgccgccg atcgcggcgc tcgtcatcgg ggcgacgaag
      841 cgcagcagcg cgggcagggc gaacagcgca aggatcatca ggcccatgcc ggtcaggatc
      901 gacacgatgc cgacaccgtt ggccccgaac gcatctgagc ccgtgagccg gaacgcggcc
      961 gcgtagatca gtgccgcggc gggcttgtag aggatgaagg cgagagtcca gccgatcgcc
     1021 ttctggaacc actggcggcc catctgcgtg ttcgtgaagc tcgccgtgat cgggagcacc
     1081 ccggcgagga tgaccagcag cccaccgcgc acgatcatga gcgcgacctg cacccatgac
     1141 atgagcatcg ccaggagggc gaggatcaga attgccagca gcgcgagcgg cgacgtcgag
     1201 ctgagcccga tcatcgcagc gacgttcgtg ccaaagcagt tcgactcccc cgcggccgac
     1261 acgtcgcatt gggtcgcgcc gttcagcacc cagaccgaga aagcgtcggt cgccgcgacc
     1321 gcaagaccga tgatcgtcag gcccgctccg gacacgacga tcaaggtcaa gagcgactgc
     1381 agcaactcct tgccggggtt cgcgcgttgc tcccacgcca tgcggatgcc ggcgatgatt
     1441 accgacacca ccgccatggc gcctacgtac caccacaagg agttctgcaa gaacccgacc
     1501 gcggaggccg accctcctgg tgcctggttc ggcagcaggc cggcgaccaa gcctgtccct
     1561 ccggatatga ggatcaccgc agccaggacg atgccgagac gaccaagcaa ctgctcaccg
     1621 tcaccacggc ggatgcgcac cgcgagcatc gccccaatgg cgatgatcga cagcacgcag
     1681 atcaccagcg cgatccacgt cacgtagccg aggatcgtgg tcacgccggc agagccagga
     1741 ggcgtgccgc ccccggcctg gcccgtcccg tttgtcagca cgggcgacgg caggtagacc
     1801 cacatcgttc cgaggctgcc gacagcctgc ccgaaccctt gaagagcctg gttcgtcagc
     1861 tcctgcaggg tgttgttcgc cgcagccgac acgatgcccg aaatgccgtc ggttatgcag
     1921 ccgaacggat ctccgatcgg atcgcaggcc atgtcaaacc cccgcccacg ggatgtatcc
     1981 gccgaggctc tcgagaggtg ccgaggcgaa cgggaacttg ccgtcgtcgg ttagcgaaac
     2041 cttccagtcg ccgtcgaccc agtgcatgac aagcgggagg ctcacgagct ggttgcccga
     2101 agaggtgact gtccacgcga cgtcgatcac cgcctcgtcg cagctgtacg agttcacctt
     2161 gaagcctgcg acttgcaggc gagagttcga cggtgccgcg tccggggatg tggctgcaac
     2221 cgcggcgtcc ctgcccttcc cgggctctac gagttcggga aggtgcggga gcgttcgagc
     2281 atccgacgcg agggccatgt agttgacgat cgcgtacagg gcgccctcgg ccgtgtgcgc
     2341 gtagcacgag cgaatgccgt cactgctgac cttgccaggg ccgaccttcg ggtcgctagg
     2401 cgccgcaacc gtgccgacta gctcccactt cgtggcgggc gcggccgtca ggctgctctc
     2461 cgtctcgaac cccggcaggc cgcacacgct cttgtcgtcg ccggatgcga ctggcccgct
     2521 cggcgtcgac gtcggctggg cgacaggagt gtgtggggcg gtgagcgcgg tcaccaggac
     2581 gaccaccccc gcgagaagga tcaccccaat tacaatcgcc gcggctatga agccgcggct
     2641 ggcgaagggg ttactcggct gtgtgctgtc gtccgacacg gttcgactcc cttgctaggt
     2701 cagctgccgg aaatgaagcc gacgatcgac gcggccgcgc cgatcaggat gcagccgagg
     2761 ccgacgtaca gcaggccacc gagttcgccg gcggcttcac cgcggcgcac gttgatcgcg
     2821 agcttgccgc cgatgatgat gacgccgagg acggcgacga tcaggccgac ccacttcacc
     2881 cagcccatga tcacggcgac cggacccgcg aagcccggag gggccaccgg gccagggtca
     2941 gggatgtcag ctggaaggga gttgatgaga tcgaacgagt gagcgagtag cgacatgagt
     3001 gtgaaccttt cagtgcttac ggagcggtgg agttgaggtg aagatcggca aggagcgcgt
     3061 gaaactcctt gggaagatca gcggccgggt cgagcgggcc gaatcgccaa gcgtcgaccc
     3121 acggcaatga ccaggtgtgc gggagcccac cggacacgac ctgcgcgaga tgccgtagtt
     3181 ccttcggcat ccggcccggc atgtccggga tgagcaccag gccgtcgacg cgtaccgcgt
     3241 cgcccagcac tccggacgcc cactcgatcg cggcccgctg cgcggccgcg aggccggcat
     3301 agttcgtgcg agccacgagg acaacgcggc agagcgagcc acggttctcc ggaatcggcc
     3361 aggcgtggtc ggccgatcgg gaacctcgag ccagagacgc cagagtggac tcccccgctc
     3421 ccccggtgac accgagccac cagaccgtcg ccccgtgcgg cgtcttccgc tgcggaagac
     3481 ggtcggcctg gtcgggcacc ggcaccccat cctgcggagc agtgacgctg gccggcttcg
     3541 ggcccgtcgt accaggcggc tcgacgatc
//
LOCUS       DAOSPL010000303         3567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26272_length_3567_cov_3.534738, whole genome shotgun sequence.
ACCESSION   DAOSPL010000303 DAOSPL010000000
VERSION     DAOSPL010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26272_length_3567_cov_3.534738"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PLQ54_06880"
     CDS             complement(<1..370)
                     /locus_tag="PLQ54_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93021.1"
                     /translation="MTEPLPIRRHRGVVRWHQGPWLLSARGPHIARSEDGGNSWTPLC
                     TVPLPPSGRVRGAFRWSARLFRAQTHHIVAVGANDLVVVAHRMVYHYDLEAGRWTRPA
                     TPLRGSRPLAMCVTPEGRVYY"
     gene            complement(363..1556)
                     /locus_tag="PLQ54_06885"
     CDS             complement(363..1556)
                     /locus_tag="PLQ54_06885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS93022.1"
                     /translation="MRAATLSSAFVRATPPDAGHPVAYLLPIPRFFSDHRGVGGHVAH
                     AGGMIGGLAQCGFEVTVLAEESHPVFDRDGVTVRLCPARSSSTLERQRWAPAFIRATR
                     DEVRVTPPLFAYIRYSVGFCPWLSRLRRALEGVPLVIEVNSFAAQDRWWMGWIERRAL
                     EMGDLIVCVSERVQDLVRARLGPGLFRRSMVVPNAVDTSRFGSECWRERRTPDELRIG
                     YAGILKPGYGLELLVDTFAAVRGQVPQAVLHVYGDGPLRGELEARAARCEGILFHGAV
                     PFLDMPRVLAGLDIAAYTMAPETSFGSSTKVYEYMAAGLPIVAADTPGVRQALGDGEH
                     GLLYPLGDGDALVRQIVRLAHDPGLARTLAQRGRDAVNAHHSWTARVQSLTSEMQRRG
                     LIRLD"
     gene            complement(1560..>3567)
                     /locus_tag="PLQ54_06890"
     CDS             complement(1560..>3567)
                     /locus_tag="PLQ54_06890"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOS93023.1"
                     /translation="LIALVMVMVVGLVAAVVFTRQPQYESQARVQFTSPKSISGMGAL
                     GSMPSLLGITFGSSSVETQRLLLIGETMVVEAAKSLGIHEPWRELKKRVKASVVPNSD
                     LIDITVFDTDPDRAADLANKVANRYIDAAREYDRQSSSEAAAYIKDEMARVREQLQHS
                     RDSVRDYKRDRKIIDVEAQTRSSMDILTRMLADQAETHGALVAAQEVADEYRRRLSEQ
                     SATYVSASTIGRNPVVTSLEQRLAELETEKAGQEAIYTAEHESVKQLEQRIAQVKREL
                     EDAVRTVVESEVTSTNPVYVDLAGKLAAAESDRRAARAREAAEEAMIAKHRRSMSSLP
                     EVELELAGLQQETSALAQVYMMLVTRYNEMKLNETLTLSNIRLVEPAKAAQEPSRPKK
                     VVNMTLGLILAFLLSIMAVGAAEALDHSVRSRAHAESAAGAPCLGMVPRAGRPEALAI
                     GADGASASFEALAISDTFHSVAARLRARCESEGAMTVLVASPAPEDGRTTVAAHLAAS
                     IARGGRRTLAIDGNLRRPALAAMWLGEGAATSPGLADVLSGLADASDVVVATDLPNLW
                     IVPPGRAASHPATLLASPTARAAIDALRGQFDAVVVDSPPASLFADSALLAGICGRVV
                     LVARVYKTPHDGLADAAARFAEAGAQVVGLVANAAPEARRERYT"
BASE COUNT          582 a         1230 c         1201 g          554 t
ORIGIN      
        1 cgtagtatac ccggccttct ggggtcacac acatggccaa cggccgcgag ccgcggagcg
       61 gagtggcggg ccgtgtccat cggcctgcct ccaggtcgta gtggtacacc attcggtggg
      121 caacgaccac caggtcgttc gcaccgacag ccacgatgtg gtgtgtctga gcgcggaaca
      181 gccgggccga ccaccggaat gcgccgcgga cgcgcccgga aggcgggagg ggcaccgtac
      241 acagcggtgt ccacgagttc ccgccgtcct cgctccgcgc gatgtgtggg cctcgggccg
      301 acaggagcca ggggccctgg tgccagcgga ccactccccg gtgacgccgg atcggcagcg
      361 gctcagtcaa ggcggatcag cccgcggcgc tgcatctcgc tcgtcagact ctgaactcgg
      421 gcggtccagg agtggtgcgc gttcaccgcg tcacgccccc gctgcgccag tgtccgcgcg
      481 agcccgggat cgtgcgccag ccgcacaatc tggcgaacca gggcatcacc atcccccaac
      541 gggtagagaa ggccgtgctc gccgtcgccc agggcctggc ggacgccggg cgtgtccgcg
      601 gcgacaatcg ggagccctgc cgccatgtac tcatagacct tggtcgacga tccaaacgac
      661 gtctccggcg ccatggtata ggccgcgatg tcgaggccag cgaggactct gggcatgtcc
      721 aggaagggca ctgctccgtg gaacaggatg ccctcgcacc gcgccgcgcg ggcctccagc
      781 tcgcctcgga gcggcccgtc gccgtataca tgaaggactg cctggggaac ttgcccgcgg
      841 acggcggcga aggtgtccac cagaagctcg agaccgtagc ccggtttgag gatgccggcg
      901 tagccgattc ggagctcgtc gggggtccgc cgctcgcgcc agcactcgga gccaaaccgg
      961 ctggtgtcga cggcgttcgg gaccaccatg gaccggcgga atagccccgg acccaggcgc
     1021 gccctgacga ggtcctgcac ccgttccgag acgcacacga tgaggtcgcc catttcgagc
     1081 gcccggcgct cgatccaccc catccaccac cggtcctggg cggcgaagct gttcacctcg
     1141 atcaccaggg gaacgccttc cagcgcgcgc cggagccggg agagccacgg acagaatcca
     1201 acggagtacc ggatgtaggc gaacagagga ggtgtcaccc gcacttcatc tcgtgtcgca
     1261 cggatgaatg ccggcgccca tcgttgccgt tcgagcgtcg acgaggaccg ggccgggcac
     1321 aaccgcaccg tcacgccgtc ccggtcgaac acggggtggg actcctcggc caacacggtc
     1381 acttcgaagc cgcactgggc gagcccgccg atcattccgc ccgcatgggc cacatgcccc
     1441 cccacccccc ggtggtcgga gaagaagcgg ggaatgggga ggaggtacgc cacgggatgc
     1501 ccggcgtccg ggggcgtggc gcgcacgaag gcgctcgaga gcgtcgcggc ccgcatcagt
     1561 taggtatacc gctcccgccg cgcttcggga gccgcgttcg ccaccagccc caccacctgc
     1621 gcacctgcct ccgcgaaccg agcggcggcg tcggccaggc catcgtgggg cgtcttgtag
     1681 acccgtgcga caagcacgac gcgcccacag atccccgcca gaagtgcgga atccgcgaag
     1741 agcgaggcgg gaggcgagtc gacaaccacc gcgtcaaact ggccgcgcaa tgcatcgatg
     1801 gcagcccgcg ccgttgggga ggccaacagg gtggccgggt ggctcgcagc ccggccggga
     1861 ggcacgatcc acagattggg caagtcggtg gccacgacga catctgaagc gtccgccagg
     1921 ccgctcagga cgtccgccag gccgggagac gtggcggcgc cctctccgag ccacatggcg
     1981 gcaagagcgg gcctgcggag gttcccatcg atggccagtg tgcgccgtcc accgcgcgcg
     2041 atcgacgcgg cgagatgggc ggccacggtg gtccggccgt cctcgggggc cgggctggcc
     2101 actaggaccg tcatggctcc ctctgattcg caccgtgcac gcagccgagc cgccaccgag
     2161 tggaacgtgt cgctaatggc gagggcctcg aatgacgcgg aggcaccgtc ggcaccaatg
     2221 gccagggcct cggggcgacc cgctcgcggc accatgccca gacacggcgc tcccgccgcc
     2281 gactcggcat gggcccgcga gcgaaccgag tggtccaggg cttccgcagc gccgaccgcc
     2341 atgatgctta gcaggaacgc caggatgagg ccgagcgtca tgttgacgac cttcttcggc
     2401 ctggacggct cctgcgctgc cttcgccggc tcgacaaggc ggatgttgct cagcgtcaac
     2461 gtctcgttga gcttcatttc attatagcgc gtgacgagca tcatatagac ttgggcgagg
     2521 gcgctcgtct cctgctgcag gccggcaagc tcgagctcga cttcgggcag ggaactcatg
     2581 cttcggcgat gcttggcgat catggcttcc tcagccgctt cgcgcgcccg cgctgcccgg
     2641 cgatcggact ccgctgccgc gagcttgccc gcgagatcca cgtacaccgg gttcgtcgag
     2701 gtgacttcag actcgactac ggtgcgcacc gcgtcttcga gctcccgctt cacctgggcg
     2761 atacgctgct cgagctgctt cacactctca tgctcggccg tgtagatggc ctcctgaccg
     2821 gccttctccg tctccagctc ggcgaggcgc tgctcgaggg aagtcaccac ggggttacgg
     2881 ccgatggtcg atgcggacac gtaggtggcg ctttgctcgc tcaggcgcct gcgatactcg
     2941 tcagcgactt cctgggcggc caccagggct ccgtgagtct ccgcctggtc ggccagcatc
     3001 cgggtgagaa tgtccatgct tgagcgggtc tgggcttcga cgtcgatgat cttccgatcg
     3061 cgcttgtagt ctcgcacgct gtcgcgggag tgctggagct gctcgcgaac ccgtgccatc
     3121 tcgtccttga tgtacgcggc ggcctcgctc gatgactgcc ggtcatactc gcgggccgcg
     3181 tcgatatacc ggttcgccac cttgttggcc aggtcggcgg cccggtcggg gtctgtgtcg
     3241 aagacggtga tgtcgatcaa gtcggagttg ggtacgacac ttgctttgac ccgcttcttc
     3301 agttcgcgcc acggctcgtg gatgcccagg ctcttggcgg cctcgaccac catcgtctcg
     3361 ccgatgagca agagcctctg ggtctcgacc gaactcgagc cgaaggtgat gcccaggaga
     3421 gacggcatac tgcccagcgc acccatgccg ctgatggact tgggcgacgt gaactgcacc
     3481 cgggcctgtg actcatactg tggttggcgg gtgaacacca cagcggccac caggcccacc
     3541 accatgacca tcaccagcgc gatcaaa
//
LOCUS       DAOSPL010000304         3558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26355_length_3558_cov_3.145304, whole genome shotgun sequence.
ACCESSION   DAOSPL010000304 DAOSPL010000000
VERSION     DAOSPL010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26355_length_3558_cov_3.145304"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PLQ54_06895"
     CDS             <1..542
                     /locus_tag="PLQ54_06895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS93024.1"
                     /translation="LVQVGMLGLWEAIMDFREDKEVSFRCFAQMCVQRELITAIKAAT
                     RQRQTPLNSSLSLDAHVYPDSPEKSLCDFLPCLKNKDPQEVWTDAESATHLKRSIRQR
                     LSGFEWKVFKAYLEGMSYNEVAGRLGCKVKSIDNALTRIRRKLSRVVLEGDEGDDLGE
                     HRLSAIAKRLETERRIAVR"
     gene            539..1420
                     /gene="glyQ"
                     /locus_tag="PLQ54_06900"
     CDS             539..1420
                     /gene="glyQ"
                     /locus_tag="PLQ54_06900"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOS93025.1"
                     /translation="MTFQQLLLALHGYWAEQGCVIEQPYDVEMGAGTMAPATFFRALG
                     PEPYRVAYVQPSRRPTDGRYGDNPYRFQRYFQYQVLLKPSPDDAQERYLESLKAVGID
                     PQVHDIRFLEDEWDAPTLGASGVGWEVWLDGMEITQFTYFQQAGSIECHPVCVEITYG
                     PERLAMYLQGVDHYAQMKWNDELTYADIRQMEERQASTYNFEHADVDRLWQLFCLFEE
                     ESRSCLDRGLTWPAYDLVLKCSHTFNLLDARGALSVTERMGVIDRVRRLARACARAYV
                     DQREALGFPLLRQPGAA"
     gene            1541..>3558
                     /gene="glyS"
                     /locus_tag="PLQ54_06905"
     CDS             1541..>3558
                     /gene="glyS"
                     /locus_tag="PLQ54_06905"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOS93026.1"
                     /translation="MSQRTFVLEVGTEELPTAWVEPALDAIGEVLCRRLADAGLAHGP
                     VRTMGTPRRLAVLIEGLAGRQADAMREDRGPPRSACFDGQGTPTKALVGFAASKGVPV
                     ESIVFRADAKGEYAYARVHVPGRTATEVLADILPDAVLAPPFPKTMRWGHRRVRYGRP
                     IQWLLALFGAEVVTFGVEDLVSGRVSRGHRFLGAPEFSIASADEYVAALEAQYVMVDH
                     RRRRAEITRQVRELAAGLGGTALYEDDLVDENLHLVEWPTALVGSFPETYLGIPHVVL
                     TTVMRKHQRYFPVADSSGCLLPYFIAVRNGGSEGTDTVRRGNEWVLVGRLEDGRFSFE
                     QDRRQRLADRVPQLGRITFVEGLGTLDDKRRRLVRLGEAVAAELGLGPSDSVALARAA
                     ELCKADLACRLVIEFPSLQGIVGGLYAEADGEPSLVAGAIRDHYLPVAVGDELPETVV
                     GRALSLVDKMDHVAGCLSLGFVPSGAGDPQGLRRRAQGMLSLVIDGRLPVDLVALFER
                     ACVELSIEAEGIRGEFMELLEQRAHAVLERHGLPRHVREAVLCDWGLLHTAVERGRVL
                     ESMLAARDAEFLDAARAATRAGNIVSKRESDRIEVDPALFEHPAEAPLLAAIRDLESS
                     LATVSADAFAERVRLFAKVAPAIDALFDGPMVMAEEPELRANRLAMLYR"
BASE COUNT          558 a         1070 c         1292 g          638 t
ORIGIN      
        1 acctggttca ggttgggatg ctcggccttt gggaagccat catggacttc cgtgaggaca
       61 aagaggtctc tttccgatgc ttcgcgcaga tgtgcgttca gcgcgagctc atcaccgcga
      121 tcaaggcggc gacccgtcaa cggcagactc ccctcaactc atccctctcg ctcgatgctc
      181 acgtttaccc cgattcgccg gagaagagcc tatgcgactt cctgccctgc ctgaagaaca
      241 aggatcccca ggaggtctgg acggacgcgg agtcggccac tcacttgaag cggagcatac
      301 ggcagcggct atcgggtttc gagtggaagg ttttcaaggc gtatctcgag ggcatgtcct
      361 acaatgaggt ggcggggcgc ctgggctgca aggtgaagtc catcgacaac gcgctcacgc
      421 ggatccggcg caagctttct cgcgtggtgc tcgagggcga cgagggcgac gacctggggg
      481 agcatcgcct atcggcgatc gccaagcgct tggagacgga gagaaggatt gccgtaagat
      541 gacgtttcag caactgctcc tggccctaca tggctactgg gccgagcagg ggtgtgtcat
      601 tgaacagcca tatgacgtgg agatgggcgc cggcaccatg gcgcccgcta cgtttttccg
      661 tgcattgggt cctgagccgt accgcgtggc ctacgtccag ccgtcgcggc ggcccaccga
      721 cggccggtac ggcgacaacc cctatcggtt tcagcgctac ttccagtatc aggtgctact
      781 caaaccatcg cccgatgatg cccaggagcg gtatcttgag agtctcaaag ccgtcggcat
      841 cgatccgcag gtgcatgaca ttcggttcct cgaggacgag tgggacgcgc cgacgcttgg
      901 agcctcgggg gtcggctggg aagtctggct cgatggcatg gagattaccc agttcacgta
      961 cttccagcag gccggctcca ttgagtgcca cccggtgtgt gtggagatca cgtacggccc
     1021 cgaacgcctg gccatgtatc tgcaaggcgt ggaccactac gcacagatga agtggaacga
     1081 cgagctgacg tacgcggata tccgacagat ggaagagcgc caggcatcca cgtacaactt
     1141 cgagcatgcc gacgtggacc ggctgtggca gctgttctgt ctgttcgagg aagagagccg
     1201 atcgtgcctc gatcgggggt tgacctggcc ggcatacgac ctggtactca agtgctcgca
     1261 cacgttcaac cttctcgatg cgcgcggcgc gctcagcgtc accgaacgga tgggcgtgat
     1321 tgaccgggtg cggcggctgg cgcgcgcatg cgcccgggcc tacgtggatc aacgcgaggc
     1381 gctcggcttc cctctgctgc gccagcccgg cgcggcgtag ggcgcggtgg gtagacgggg
     1441 gcgacctggc ggtctctccc acggcgcccc aacggaacgg agatcggggg ctgacctggc
     1501 gacgggttgg cccctcactg gtatgcaggg agatcgcatc gtgtcgcagc gcaccttcgt
     1561 gttggaggtc gggactgagg agttgccaac ggcgtgggtc gagccggcgc tcgatgccat
     1621 cggcgaggtc ctgtgtcggc ggctggccga tgcggggctt gcccatggcc ccgtgcggac
     1681 catgggaaca ccgcgccgcc tggccgttct catcgaggga ctggcgggac gccaggccga
     1741 cgccatgcgg gaggaccgtg ggccgccccg gtcggcgtgc ttcgatgggc aggggacgcc
     1801 gacgaaggcg ctggtggggt tcgcggcgtc caagggcgtc cccgtggagt ccatcgtgtt
     1861 ccgtgcggac gcgaaggggg agtacgcgta tgccagggtc catgtgccgg ggcgcacagc
     1921 gaccgaggtc ctggctgaca tcctgccgga tgcggtactc gccccgccgt tccccaagac
     1981 gatgcgctgg gggcatcggc gcgtgcgcta cgggcggccc atccagtggc tgctggcgct
     2041 gtttggcgcg gaggtcgtga ccttcggggt cgaggacctg gtgtcgggcc gggtgagccg
     2101 gggccatcgg ttcctgggcg cgcccgagtt ctccatcgcg tcggccgatg agtacgtggc
     2161 ggcgctggaa gcccagtacg tgatggtgga ccaccggcgg cggcgggcgg agatcacgcg
     2221 tcaggtacgc gagctcgctg cgggccttgg tggcactgcg ctctacgagg acgacctcgt
     2281 cgacgagaac ctgcacttgg ttgagtggcc cacggctctt gtgggcagct tcccggagac
     2341 ctaccttggc atcccccacg tggtcctgac cacggtcatg cggaaacatc agaggtactt
     2401 cccggtggcc gattcctcgg gctgcctgct tccgtacttc attgccgtcc gcaacggcgg
     2461 tagcgagggg accgacaccg tccggcgggg gaacgagtgg gtgttggtgg gccggctgga
     2521 ggatggacgg ttctcattcg aacaggatcg ccgccagcgg ttggccgacc gtgtgccgca
     2581 actcggtcgc atcacatttg ttgagggact cggcacgctg gacgacaaga ggcggcgcct
     2641 ggtccgtctg ggcgaggccg tggcggccga actgggcctt ggcccgagcg atagtgtggc
     2701 gctggcgcga gccgcggagc tgtgcaaggc ggacctggcg tgtcggctcg tgatcgagtt
     2761 cccatcgttg caggggatcg tgggcgggct ctatgccgag gcggacggcg agccgtcgct
     2821 ggtggcgggg gcgatccgag atcactacct tccggtggcg gtgggggacg aactcccgga
     2881 gaccgtggtt ggacgcgcgc tgagcttggt ggacaagatg gaccacgtgg ccggatgcct
     2941 gagcctgggc ttcgtgccca gtggggccgg agatccccag gggcttcgcc gacgcgcgca
     3001 gggaatgttg tctctggtca tcgacggaag gctgccggtg gacctcgtcg ccctgttcga
     3061 gcgagcgtgc gtcgagctgt cgatcgaggc cgagggcatc cgtggggagt tcatggagct
     3121 gctggagcag cgggcacacg ccgtgctcga gcggcacgga ctgccgaggc acgtgcggga
     3181 agcggtgctc tgcgactggg gtctgctgca cactgccgtt gaacgcggga gagtactgga
     3241 gtcgatgctt gccgcgcgcg atgccgagtt cctcgacgcc gctcgggcgg cgactcgggc
     3301 ggggaacatc gtctcgaagc gcgagtcgga ccgcatcgag gtggatcccg cgctgttcga
     3361 acaccctgcc gaggcaccct tgcttgcggc cattcgggat ctcgagtcct cactcgctac
     3421 ggtgtcggcc gacgcgttcg ccgagcgtgt acggttgttc gccaaggtcg cgcccgccat
     3481 cgatgccctg ttcgatggcc ctatggtcat ggccgaggag cctgagctcc gggcgaatcg
     3541 gctggcgatg ctgtaccg
//
LOCUS       DAOSPL010000305         3556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26369_length_3556_cov_3.145958, whole genome shotgun sequence.
ACCESSION   DAOSPL010000305 DAOSPL010000000
VERSION     DAOSPL010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26369_length_3556_cov_3.145958"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2387
                     /locus_tag="PLQ54_06910"
     CDS             <1..2387
                     /locus_tag="PLQ54_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOS93027.1"
                     /translation="GGSMDMSAAGKSGSSARRGWSMGSRVIALVLVSIAASAAGPAYL
                     AYRAFSAYAVTSAGTELQRYGGEVLLRSGGLVEGHILALQALATSPDVVSIVRSSNAA
                     NAERDPAELEAWIAATDKAWAENLPQVADTVESVVGNSVSSHFKSFISAVAGQVEVFV
                     TDSEGVAVAESNRTSDLMQADEDWWQATWAGGSGAVFIGDVEYDESSKAWGMDIGVPV
                     REPGGKAIGVLRGTVNVSVLVDALGQIVIGQEGHVGLLAKDATIISAPGHEASGDEKT
                     PAPAGIAALAQEGKSTWREGIPDFSGQPAVVAVATMKEGMASSLGWVLVATLPLAEAH
                     APALAASLNSVKVTALVAVVLMVFALVSGRALAAPLRSATEAARALGIGDSERAVELV
                     TRFACRRDEVGELSRAFVELAARTSAIAKVAHQVAGGDLTASVTARSDRDELSLAVAE
                     MVEQMRDLVGQVAESTRQLLEGVGQVDEAMSQVARSAMSQTESVETATNAMERLMQGV
                     GDVAAGAREQAESVGRCSASTDAISAGLAGVLQVAQAGAQAARAAADKARGAAQTVRA
                     TAEGMNDIREGTDLAAQRVREMGERSEQIGEIIETVEDIAAQTNLLALNAAIEAARAG
                     EHGRGFAVVADEVRKLAEGAGSAAQEIGGLVRTMREAIEQAMAAMNRGNAEVEKGMAQ
                     ATRASEAIDGILAEVDDVARQVQEITSAAEAMADGARDLVSVMGDLAAIVERYSEATD
                     TIEAEATGISDQMSGIVAISEENSAAAQEVAATTDLLRGMAGQVDALVGRFRLS"
     gene            complement(2611..>3556)
                     /locus_tag="PLQ54_06915"
     CDS             complement(2611..>3556)
                     /locus_tag="PLQ54_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93028.1"
                     /translation="DAGGRSDALLSGYVTRVLFAAPTVKGGQVVPDPARLVHGRTVSV
                     HKLSYRWLQGKYLTRQDEFELLASGDRELVRTLVSWNAGEGYGSTIEESVIGRGLWED
                     ERVDATAVAQINESPRESEGWLRGYLQLDTSGQGLATTYARAEVVGIEDVDGLECVHL
                     VSDTTDPESGLWGAEHVWVAPAHGYRRARREAFVALPDGRVNTSVEMVTAFEEVVPGL
                     WMPAEIHSVGYLRKPTGEWTWTRAAHVICHSAKAPNPGALLLTPLMPNNTLLLWPDGV
                     QDHWGHNTVDLEASILAGQTPFDFALPELRSGLPEAIR"
BASE COUNT          567 a         1100 c         1313 g          576 t
ORIGIN      
        1 gagggggatc gatggacatg tcggcggcgg gtaagagtgg cagttcggca cgtcgcgggt
       61 ggagcatggg ctcccgcgtg atcgcgctgg tcctggtctc gatcgcggcc tcagccgcgg
      121 gccccgcata cctcgcctac cgtgccttct cggcctacgc cgttacgagc gctggcacgg
      181 agctgcagcg gtatggcggc gaagtgctac tgcggtcggg cggtctggtc gaggggcaca
      241 tcctggcgct ccaggcgttg gcgacctcgc ccgatgtggt cagtatcgtt cggagctcga
      301 atgccgccaa tgctgagcgg gaccctgcgg agctcgaggc ctggatcgcg gccaccgaca
      361 aggcctgggc cgagaacctg ccccaggtgg ccgacacggt cgagtcagtt gttggcaact
      421 ctgtcagcag ccacttcaag agcttcatca gtgcggtcgc gggccaggtc gaagtcttcg
      481 tgacggactc tgagggcgtg gccgtcgccg aaagcaaccg cacgagcgac ctcatgcagg
      541 ctgacgagga ctggtggcag gccacttggg ccgggggctc gggggctgtc tttatcggcg
      601 atgtcgagta cgacgagagc tcgaaagcct gggggatgga catcggcgta ccggtgcgcg
      661 agccgggagg gaaggcgatc ggtgtcctgc gcgggaccgt caatgtgtcg gtgctggtgg
      721 atgcactggg gcagatcgtg atcggtcagg agggtcatgt gggcctccta gccaaggacg
      781 ccacgatcat ctccgccccg gggcatgagg ccagtggcga cgagaagacc ccggcgcctg
      841 ctggcatcgc cgcgctggct caggagggca agagcacgtg gcgtgagggc atacccgact
      901 tctccggcca accggctgtc gtcgccgtgg cgaccatgaa ggaggggatg gcgagcagcc
      961 tggggtgggt gctggtggct accctgccac tggcggaggc ccatgctccg gctctcgcgg
     1021 cgtcgctcaa cagtgtgaaa gtgaccgcat tggtggcggt cgttctgatg gtgttcgctt
     1081 tggtgtccgg ccgggctctc gcggcgcctc tgcgctccgc tacggaggcg gctcgcgcgc
     1141 tgggcatcgg ggattccgag cgtgcggtgg agctcgtgac ccggttcgct tgccgtcgcg
     1201 acgaggtggg tgagctgagt cgtgccttcg tcgagcttgc cgctcgcact tcggccatcg
     1261 ccaaggtcgc gcatcaagtg gccggtggcg atctcactgc ctccgtcacc gcccggagcg
     1321 accgggacga gctgagcctg gctgtggcgg agatggtcga gcagatgcgg gacctcgtcg
     1381 gccaggtcgc cgagagcacg cgccaactgc tggagggcgt tggccaagtc gacgaggcca
     1441 tgtcgcaggt ggcgcgaagt gccatgagtc agaccgagag cgtcgaaacg gcaaccaatg
     1501 ccatggaacg cctgatgcag ggggtgggtg acgtggccgc gggcgcccgc gagcaagctg
     1561 aatcggtggg gcgatgtagc gcgtcgaccg acgcgatctc agctggcctg gcgggcgtgt
     1621 tacaggtcgc gcaggcgggc gctcaggcgg cccgcgcggc tgcggacaag gcgcgtggtg
     1681 ccgcccagac tgtccgggcg acggctgaag gcatgaacga catccgcgag ggaacagacc
     1741 ttgccgcgca acgggtccgc gagatgggcg aacgttcgga gcagatcggc gagatcatcg
     1801 agaccgtgga ggacatcgcg gcccagacga acttgctggc tctcaacgcg gcgatcgagg
     1861 cggcccgtgc cggcgagcac gggcgcggct tcgccgtggt cgccgacgag gtgcgcaagc
     1921 tggctgaggg ggccggatcg gcagctcagg agatcggcgg cctcgtccgc accatgcgtg
     1981 aggccatcga gcaagcgatg gctgccatga accggggcaa tgccgaggtg gaaaagggca
     2041 tggctcaggc cacgcgggcc agtgaggcga tcgacggcat tctcgccgag gtcgacgatg
     2101 tggcgcgcca ggttcaggag atcacctcgg cggcggaggc catggcggat ggggctcgcg
     2161 acctggtgag cgtaatgggt gatctggccg ccattgttga gcggtactcg gaggccactg
     2221 acaccatcga ggcggaggcg acgggtatct cggatcagat gagtggcatt gtcgcgatct
     2281 cagaagagaa cagcgccgcc gcccaagagg tggccgcgac aaccgatctc ctccgcggga
     2341 tggcagggca ggtggacgct ctggtgggcc gcttccggct gagctgagcg cgatggcgca
     2401 gccgatggct gccgtggcct agcgggctgg cttcctccca acggtcgccc gatagatgat
     2461 gcactgctcg tcggggtcca ggtcgaggag cgtggccatg ggtgaccggg gactgagccc
     2521 tctgccacga cgggcattgg cgtgaggcag cgccccccat ggcggagggc tctgtcccca
     2581 tgggcgtggg cggctacctg gggctctcgc ctaacggatc gcctccggca ggccggaccg
     2641 tagctcagga agcgcgaagt cgaacggcgt ctggccggcg aggatgctgg cttcgagatc
     2701 gacggtgttg tgcccccagt ggtcctgcac gccgtcaggc cagagcaaga gcgtgttgtt
     2761 cggcatcagt ggtgtgagca gcagggcccc tgggttgggt gccttggctg agtggcagat
     2821 cacatgggcc gcacgcgtcc acgtccactc gccagtaggc ttgcgcaggt agcccacgct
     2881 gtggatctcg gcgggcatcc agaggcccgg gacgacctct tcgaaagccg tgaccatctc
     2941 gacgctggtg ttcacgcggc catcggggag agcgacgaac gcctcgcgtc gcgcgcgccg
     3001 gtatccgtgg gcgggggcca cccatacgtg ctctgctccc cagagcccgg actcaggatc
     3061 ggtggtgtcc gaaaccaggt gaacgcactc caggccatcg acgtcctcga tgccgacgac
     3121 ctccgcgcgg gcgtacgtgg ttgccagtcc ttgtccagac gtatccaact gcaggtaccc
     3181 gcgcaaccac ccttcggact cgcgcggcga ctcgttgatc tgagccacgg cagtcgcgtc
     3241 cactcgttcg tcttcccaga gaccgcggcc gatcacgctc tcctcgatcg ttgagccgta
     3301 gccctccccg gcgttccagg agacgagagt ccgcacgagc tcccgatccc ccgacgccag
     3361 cagctcgaac tcatcttgcc gcgtcaggta cttcccttgg agccagcggt agctcagctt
     3421 gtgaaccgag acggtgcgcc cgtgaacgag ccgggccgga tctgggacca cttggccgcc
     3481 cttgacggtc ggcgccgcga ataggacccg cgtcacatag ccggagagca gcgcgtcact
     3541 gcggccaccg gcgtct
//
LOCUS       DAOSPL010000306         3547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26466_length_3547_cov_2.989118, whole genome shotgun sequence.
ACCESSION   DAOSPL010000306 DAOSPL010000000
VERSION     DAOSPL010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26466_length_3547_cov_2.989118"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..1140
                     /locus_tag="PLQ54_06920"
     CDS             52..1140
                     /locus_tag="PLQ54_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93029.1"
                     /translation="MIQRTYGQVKDFLSTYALNNGVPVDDTRLIDYTNRAVRELMNEG
                     DWPGVVDTWYLRYDSASGMLTLPGHLDLLLNVSIDDTPKEIRSPWFEFVQYGPGSVRD
                     EEKDSYGNTLGKRQNWTGWLVDRGMACTQYELPTKSEDSATQAAAPWYLRVYATVDED
                     IDSVSPVLHVTGLDEDGHPVRTTPDGGTTWISGENLDIDFAAVGGFTQSTHAYSQISA
                     IVKPVTNQAVRLTAWNGTTEIELSRYEWDETTPAYRRYYVPELAKPAASLGLRDRVVR
                     ARCRRRFIPVAADNDVLIIGNELALVEMMIAQWKRQNGNVDEYASHKMVAVDLMRKES
                     LAHNGKTRTPAITFSRGWGLGSDFNPVR"
     gene            1359..1559
                     /locus_tag="PLQ54_06925"
     CDS             1359..1559
                     /locus_tag="PLQ54_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93030.1"
                     /translation="MAYEDLAGPVGADAKFAVGAPFAAGTGVFFEAYPLGEGVLRLVA
                     VNLSGATVTLPGTNFTLFILNG"
     gene            1552..3288
                     /locus_tag="PLQ54_06930"
     CDS             1552..3288
                     /locus_tag="PLQ54_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93031.1"
                     /translation="MAKVTPAWLSEGFTSALTGVNSAVAPDLVGTSALAWGYNVANRG
                     SRPHTRPYFIRRMTLPSGRVQGASYFGVQGGMGVAVIDGRMYRLRPGEARDSSDSSYE
                     EISLPSLGSSSTQRVWMCQTVESLVVQDFLNAPIIYDGSSARLAGALEVPRGKQMAYG
                     NGRLWVAVNDTTLAAGNIRTATPGSELVFTETNYLSGGGTFTFPDGITALGFIATTGT
                     SDYGSLLVFSGNRVDSLRADITARDDWQSMPGFITTVLRHGGTPGQTCLAEVNQDIYW
                     RDSDGGIRSVRSSLADESGAGNTPISREVSRLTDFDTKDRLIDCPAVNFNNRLLVGSS
                     PYINFYGRTAWKSLIALDFAPVSTMAGKSSPAYDGEWTGLGVTHMFAGRIGGKTRCFM
                     VSCSDAGENSLWEVLDDNSGEIADRTLDCSTNELVDSRVTSYFETARRSFDTLGTRKR
                     LERIDAYVSGILGRVDMSIYWRADQNQKWRLADSMVFCAQVNDPEGATPHVFKNLVAQ
                     HRSQVKTFTLAPSRDLISKYATHIGFEFQFRVVWTGHCEVTRIVAHASPLDESVYAHR
                     VWDDSCVPEDVT"
     gene            3285..>3547
                     /locus_tag="PLQ54_06935"
     CDS             3285..>3547
                     /locus_tag="PLQ54_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93032.1"
                     /translation="MSNTVGYSLGCVTSVKYNLTQGEDATGNGLGYALGGCPGCWNGD
                     YRDRRYWAQIPLSATATSGTDYYGALAPTLKFQGGGTYREICPT"
BASE COUNT          606 a         1236 c         1111 g          594 t
ORIGIN      
        1 cctgtgacgg tggcttgact cccgccgccc tcaaggcata agttccctgg cgtgatccag
       61 cgcacctacg gacaggtcaa ggacttcctc tccacctacg ccttgaacaa cggggtgcca
      121 gtggatgaca cccggctgat cgactacacc aatcgcgccg tgcgggagct gatgaacgag
      181 ggcgactggc cgggcgtcgt ggacacatgg tacctgcgct acgactccgc cagcgggatg
      241 ctcacgcttc ccgggcactt ggatctcctg ctcaacgtct ccatcgacga cacccccaag
      301 gagattcgca gcccgtggtt cgagttcgtg cagtacggcc cgggcagtgt gcgcgacgag
      361 gagaaggaca gttacggcaa caccttgggc aaacggcaga actggacggg gtggctggtg
      421 gatcgcggca tggcctgcac gcagtacgag cttcccacca agtccgagga ctccgccacc
      481 caagccgccg cgccgtggta cctccgagtc tacgccaccg tggacgagga catcgactcc
      541 gtctccccgg tgctccacgt cacgggactg gatgaggacg gccacccggt caggaccacc
      601 cccgacgggg gtacgacttg gatctcgggc gagaacctcg acatcgactt cgcggccgtc
      661 ggcgggttca cccaatccac ccacgcctac tcgcagatca gcgccatcgt caagccggtc
      721 accaaccagg cggtcagact gacggcttgg aacggcacca cggagatcga gctttcgcgc
      781 tacgagtggg acgagaccac ccccgcctac cgccgctact acgtccccga gttggccaag
      841 cccgcggcct ccctcggcct cagggaccgg gtcgtccgcg ctcggtgccg gcgtcgcttc
      901 atccccgtcg ccgctgacaa cgatgtgctc atcatcggca acgagctggc gctggtcgag
      961 atgatgatcg cgcagtggaa acggcagaac ggcaacgtgg acgagtacgc cagccacaag
     1021 atggtcgccg tcgatctcat gcgcaaggag tccctcgccc acaacggcaa gacccgcacg
     1081 ccggccatca ccttctcccg aggctggggg ctgggcagcg acttcaaccc tgtgcgatga
     1141 aagtcacccg agcagactgg gcggggtcgg tcccttggtc cggcatcacc ggcaagccgg
     1201 cagggctcgc cggctccgct gtcacggacc tgaccgcact gaccgcccgc gtcgcggcgc
     1261 ttgaggcggc tgtcaattcc ctctcttccg cccctgccgg ctcctcgctc gcgtcccttg
     1321 atgtctggtg ggcggtcggc accttagagc ccctccagat ggcctacgag gacctcgcgg
     1381 gtccggtcgg ggccgatgcc aagttcgcgg tcggggcgcc tttcgccgca gggaccgggg
     1441 tgttcttcga ggcgtatccc ctcggagagg gggtcctccg cctcgtggcc gtcaacctat
     1501 caggggctac ggtgacgctc ccggggacca acttcaccct tttcatcctc aatggctaag
     1561 gttaccccag cttggctctc cgagggattc acctccgctc tcacgggggt caactccgcc
     1621 gtcgcccccg acctcgttgg cacctcggcc ttggcgtggg gctacaacgt cgccaaccga
     1681 ggtagccggc cgcacacccg tccctacttc atccgacgga tgactctccc ctcgggccgg
     1741 gtgcaggggg cgagctactt cggggtgcag ggcggcatgg gggtggccgt aatcgacggt
     1801 cggatgtacc ggctgcggcc gggggaggcc cgcgactcgt ccgacagctc ctacgaggag
     1861 atttcgctcc cgtcgctcgg ctcgtcgtcc acccagcgcg tctggatgtg ccagacggtc
     1921 gaaagcctcg tggtgcagga tttcctgaac gccccgatca tctatgacgg ctcctccgcc
     1981 cggctggccg gggcgctgga ggtgccccgg ggcaagcaga tggcttacgg caacgggcgg
     2041 ctgtgggtgg cggtcaacga caccacgctg gcggccggca acattcgcac cgctacgccg
     2101 gggtcggagc tggtcttcac cgagaccaac tacctctccg gtggcggcac cttcaccttt
     2161 cccgacggca tcaccgccct cggcttcatc gccaccaccg gcacgagcga ctacggctcc
     2221 ctgctggtct tcagcggcaa tcgggtcgat tctctccgcg ccgacatcac cgcccgcgac
     2281 gactggcaat ccatgcccgg gttcatcacc acggtcctgc ggcacggagg cacgccgggg
     2341 cagacctgcc tcgccgaggt caaccaggac atctactggc gcgacagtga cggcggcatt
     2401 cgcagcgtgc ggtcctcgct ggccgacgag tccggtgccg gcaacacgcc catcagccgc
     2461 gaggtgtcac ggctcaccga cttcgacacc aaggaccggc tgatcgactg cccggcagtc
     2521 aacttcaaca accgcctgct ggtgggctcc tcgccctaca tcaacttcta cggtcggacg
     2581 gcgtggaaat ccctcatcgc cctcgacttc gcacccgtca gcacgatggc gggcaaatcg
     2641 agcccggcct acgatggcga gtggaccggg ctcggggtca cccacatgtt cgccgggcgc
     2701 atcggtggaa agacacgttg cttcatggtg tcctgctcgg atgcgggcga gaactccctg
     2761 tgggaggtcc ttgacgacaa cagcggcgag atcgccgacc gcaccctcga ctgctcaact
     2821 aacgagttag ttgactcacg ggtgacgagc tacttcgaga cggcgcggcg gtccttcgat
     2881 accctcggga ctcgcaagcg cctggagcgg atcgatgcct acgtctctgg gattctcgga
     2941 cgggtggaca tgagcatcta ctggcgggcc gaccagaacc agaagtggcg gctggccgac
     3001 agcatggtct tctgcgctca ggtcaacgat cccgaggggg cgacgccgca cgtcttcaag
     3061 aacctcgtgg cccagcaccg cagtcaagtg aagactttca cgctggctcc cagccgcgat
     3121 ctcatcagca agtacgccac ccacatcggc ttcgagttcc agttccgggt cgtctggacc
     3181 gggcactgcg aggtcacccg catcgtggcc catgccagcc cgctcgacga gtccgtctac
     3241 gcccaccggg tctgggatga ctcctgcgtc ccggaggatg taacgtgagc aacaccgtcg
     3301 gctactccct cggctgcgtt acctcggtga agtacaacct cacgcaagga gaggacgcga
     3361 cggggaacgg cttggggtat gccctcgggg gatgcccggg gtgctggaat ggcgactacc
     3421 gagatcggcg gtactgggcg cagatccccc tgtcggccac cgccacctcg ggcacggact
     3481 actacggcgc cttggctccg actctgaagt tccaaggtgg aggcacctat cgggaaatct
     3541 gccccac
//
LOCUS       DAOSPL010000307         3543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26508_length_3543_cov_2.876147, whole genome shotgun sequence.
ACCESSION   DAOSPL010000307 DAOSPL010000000
VERSION     DAOSPL010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26508_length_3543_cov_2.876147"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PLQ54_06940"
     CDS             <1..576
                     /locus_tag="PLQ54_06940"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOS93033.1"
                     /translation="CPNGALELVPALTGHCYVSGTPYGPLAHARLRPGGGNSGKLVTE
                     VRALAQETATERAAPLVIIDGPPGIGCSAIAAIADADMVLIVAEPTASSLHDLERVVK
                     LVRHFGLPAAVILNKADLSEPQAAAVRGFCSVQEVPLVGEIPFDETVPKAVSQGVPVV
                     TVPGSPAGDALREIWKWVARALGADGSPSLS"
     gene            complement(719..1282)
                     /locus_tag="PLQ54_06945"
     CDS             complement(719..1282)
                     /locus_tag="PLQ54_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93034.1"
                     /translation="MRHHSLWSVVVGATLGLLGLAVHGSVSAQQAGRVPQVRAAEGHA
                     LKTITLNTGAYSVEPGTDRRSESIVATEDMHIVSISHFTGVQTGAFPSDNGHVLSTSP
                     ENPWEKWADKATGMEPTGTRGYFGYCGRDYYSECTGIGDIMWQETMPAGCHVLVRAGE
                     TLYMHTYTGNGTGGPRMFHHLARVYYW"
     gene            complement(1391..2284)
                     /locus_tag="PLQ54_06950"
     CDS             complement(1391..2284)
                     /locus_tag="PLQ54_06950"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOS93035.1"
                     /translation="MLPIAAALCLGTVWGDDEVLLTGQIHMRDPWVLPYDGIYHLIGT
                     TGDAWGKEGGGFIGYRSTDLVHWTVTGQALPAEGKPAWADYHFWAPELYERDGRFFLC
                     FSGKTDHSCRGTGMAVSDSPLGPFRPLSDMPLTPPDWECLDGQLFRDTDGTEWFLFVH
                     EWVQAPLGEMWIQQISPDYTQLVGERHLLFRGEDAPWSNKVIDGPMMVAEDGKYHLFW
                     SSFNDRDGYCCGVATADSLLGPYTQSPEPVIRDDGGHNCVFRGFDGQLYTSFHRPNNT
                     PDERVCIQKLEYVDGKWGLGE"
     gene            complement(2339..>3543)
                     /locus_tag="PLQ54_06955"
     CDS             complement(2339..>3543)
                     /locus_tag="PLQ54_06955"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOS93036.1"
                     /translation="AATIEALNWGLEVVGILDGFKWLSKNDTSHTVKLSYADVEQTRF
                     RGGSMLHTSRENPTKDEQKMQNVVAALKTLGIDYLVTIGGDDTAFSASKTSQYGAGAI
                     KVAHVPKTIDNDLPLPGNMPTFGFQTARHYGGELVRNLVNDARTTRRWYLVVAMGRTA
                     GHLALGMGTCGDANLTIIPEELPDEGYSLDLLCDTIECAMLKTKVTRGDDCGVVVVAE
                     GVAEFCAEDLKDHPLVALKYDEHGHWRLAEVPFALILKRKLQERAEARGEKTTYVDVT
                     VGYELRCSDPISVDVQYTEQLGWGAVRYLLGIGEESYERGGAMISIQAGDIVPIPFSD
                     ILDPKTGKTSVRRVNVHSDYYRCARSFMTRLEREDLSNEATLGKLAEAAGMAPEDFRA
                     KFGPVVGM"
BASE COUNT          564 a         1207 c         1150 g          622 t
ORIGIN      
        1 tgccccaacg gggcccttga gctcgttccg gctctcacgg gccactgcta cgtgtccggc
       61 acaccatacg gtcccctggc ccacgctcgg ctccggccgg gaggcggcaa ctcgggcaag
      121 ctcgtgacgg aagtccgtgc cctcgcccag gagacagcca cggagcgggc cgccccactc
      181 gtgatcatcg atggcccgcc cggcatcggc tgcagcgcca tcgcggccat tgccgacgcc
      241 gatatggtgc tcattgtcgc cgagccgacg gcctcaagcc ttcacgacct cgaacgcgtg
      301 gtcaagctgg tgaggcactt cggtctgccg gctgcggtga tcctcaacaa ggccgatctg
      361 agcgaaccac aggcggcggc cgtgcgaggt ttctgttcgg ttcaagaggt gccactggtg
      421 ggcgagattc ccttcgacga aaccgtgccg aaggcggtct cacaaggggt accggtcgtg
      481 acagtacccg gttctcccgc aggcgatgcc cttcgggaga tctggaagtg ggttgcgcga
      541 gccctcggcg ccgatggctc cccgtccctc agctaggcgc ttctactgag gggcagtcgg
      601 cgtccactgg ggcggcttgg ctggagagga tccggcccat acctcgccaa gacgcccggc
      661 gagacggggc aggcgcgggt tcgcggacga cggtggaccc gtgtctgccc ctcgcgtcct
      721 accagtagta gactcgggcc aggtggtgga acatccgcgg cccaccggtt ccgttgccgg
      781 tgtaggtgtg catgtagagc gtctcgccgg cgcgcacgag cacatggcac ccggcgggca
      841 tcgtctcctg ccacatgatg tcgccgatgc ccgtgcactc gctgtagtag tcgcgcccgc
      901 agtaaccgaa gtagccccgg gtgcccgtcg gctccatgcc cgttgctttg tcggcccact
      961 tctcccaagg gttctccggc gaggtcgaca gcacgtgccc attgtccgag gggaacgcac
     1021 cggtctggac gccggtgaag tggctgatcg acacgatgtg catgtcctcc gtggcgacaa
     1081 tgctctcgct gcggcggtcg gttcccggct ccacggagta ggccccggtg ttgagggtga
     1141 tggtcttcag cgcatgcccc tcggccgcgc gcacctgcgg tactcggccc gcctgttgcg
     1201 cggatacaga gccgtgcact gccaggccca ggagtcccag ggtggcaccg acgacaacgg
     1261 accagagcga atggtgacgc atcgccacat ctcccccttg tgtggagtca tgtggcggga
     1321 gttcggcgac gactcgaagc tggccttgcg gaatccgggg ggcagacacc ggtctccggg
     1381 cgcggttgtg ctactccccc aacccccact ttccatcgac gtactcaagc ttctgaatgc
     1441 atacccgctc atctggtgtg ttgttcggcc ggtggaaact cgtgtagagc tggccgtcga
     1501 acccgcggaa cacgcagttg tggccgccat cgtcccggat gaccggctcg ggcgactgcg
     1561 tgtaggggcc cagcaatgag tcggccgtgg ccaccccgca gcagtagccg tcccggtcgt
     1621 tgaagcttga ccagaacagg tggtacttgc cgtcttcagc caccatcata gggccatcga
     1681 tgaccttatt ggaccacggg gcatcctcgc cccggaacag caggtgcctc tcgccaacga
     1741 gctgcgtgta gtcgggcgag atctgctgga tccacatctc gcccagcggg gcctgcaccc
     1801 actcgtgaac gaagaggaac cattcggtgc cgtcggtgtc gcggaacaac tgcccgtcga
     1861 ggcactccca gtccggtggg gtcagtggca tgtcggacag cggccggaac ggcccgagcg
     1921 gtgagtcgga gaccgccatg cccgtgccgc ggcacgagtg gtctgtcttg cccgagaagc
     1981 agaggaagaa ccgcccgtcg cgctcataaa gctcaggggc ccagaagtgg tagtcggccc
     2041 acgcgggctt cccctcggcg ggcaaggcct gccccgtgac ggtccagtgt accaagtcgg
     2101 tcgaacggta gccgatgaag cccccgccct ccttgcccca ggcgtcgccc gtggtcccga
     2161 tgaggtggta gatcccgtca tagggcagga cccacgggtc gcgcatgtgg atctggccgg
     2221 tgagcagcac ttcgtcgtcc ccccagaccg tgccgagaca gagagcggcg gcgataggca
     2281 gcacggccgc cgctctcggc ccacgaagcc tccgactccg tcccgcagtc acagagccct
     2341 acatccccac cacggggccg aacttggccc tgaagtcttc cggcgccatc cccgctgcct
     2401 cggccagctt gcccagcgtg gcctcgttcg agaggtcctc gcgctcgagg cgcgtcatga
     2461 acgagcgggc gcagcggtag tagtccgagt gcacgttcac acggcgaacg gacgtcttgc
     2521 cggtcttggg gtcgaggata tcgctgaacg ggatcggcac aatgtccccg gcctggatcg
     2581 agatcatggc tccgccgcgc tcgtagctct cctcgccgat ccccagcaag taccgcacgg
     2641 cgccccatcc cagttgctcg gtgtactgca catcgaccga gatggggtcg gagcaacgca
     2701 gctcgtagcc cacggtgacg tcgacatagg tcgtcttctc gccccgggcc tcagcccgct
     2761 cctggagctt acgcttgagg atcagcgcga aaggcacttc ggccagccgc cagtgcccgt
     2821 gctcgtcgta cttgagggcg accagagggt ggtccttcag gtcctcggcg cagaactccg
     2881 ccacgccctc ggccaccacc acgacgccgc agtcgtcgcc gcgggtcacc ttcgtcttga
     2941 gcatggcgca ctcgatggtg tcgcacagca ggtccagcga gtagccctcg tcgggcagct
     3001 cctcggggat gatggtcagg ttcgcgtcgc cgcaggtgcc catgcccagc gccaggtgac
     3061 ctgccgtccg ccccatggcc acgaccaggt accagcgccg agtcgtccgg gcgtcgttca
     3121 ccaggttccg caccagctcg ccaccatagt ggcgggcggt ctggaaaccg aaggtcggca
     3181 tgttgcccgg cagcggcagg tcgttgtcga tggtcttcgg cacgtgggcc accttaatgg
     3241 cacccgcccc atactgggat gtcttgctgg cgctgaatgc ggtgtcgtct ccgccaatgg
     3301 tgaccaggta gtcgatgccc aatgtcttga gcgcggccac cacgttctgc atcttctgct
     3361 cgtccttggt ggggttctcg cgcgacgtgt gcagcatcga cccgccacgg aaccgcgtct
     3421 gctcgacgtc ggcatacgaa agcttcacgg tgtggctggt gtcgttcttc gatagccact
     3481 tgaagccgtc caggatgccg accacctcaa gcccccagtt cagggcctcg atggtcgcag
     3541 cgc
//
LOCUS       DAOSPL010000308         3537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26561_length_3537_cov_3.285468, whole genome shotgun sequence.
ACCESSION   DAOSPL010000308 DAOSPL010000000
VERSION     DAOSPL010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26561_length_3537_cov_3.285468"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLQ54_06960"
     CDS             <1..245
                     /locus_tag="PLQ54_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HOS93037.1"
                     /translation="RADRLRSLAQIWDEMRARLRADLPTVPEHRQRLEAAGAPTAPEQ
                     VGQSPESARAAVRYGRYLRARYTVLDLAADLAVLED"
     gene            256..921
                     /gene="nth"
                     /locus_tag="PLQ54_06965"
     CDS             256..921
                     /gene="nth"
                     /locus_tag="PLQ54_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOS93038.1"
                     /translation="MPGATELDATTRARKIVARLIQEYPDARCALDYTNPLELLIATI
                     LSAQCTDERVNIVTKDLFIKYTRAIDYAVATQEQLEQDIKSIGLFRRKAQSLRATCQA
                     LVERFDGEVPDTLEELVTLPGVARKTANVVLGNAFGVTSGVVVDTHVFRLAHRMGLTT
                     QLYADKVEEDLMALLPKKRWVMAAHVLIFHGRQVCTALRPHCAECVVARWCPRLLDAP
                     KPK"
     gene            1005..2399
                     /locus_tag="PLQ54_06970"
     CDS             1005..2399
                     /locus_tag="PLQ54_06970"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS93039.1"
                     /translation="MKRGISLCVVVAGVVAAAGAWAAPFVCVLPGPDGDPALSPGALA
                     VAESLLRRDPALAMANRRQAQWVLAQAGVAAGRPPTGAQAEQACDRIGCSAAVLVSLR
                     ADGADYLARASVLVAGQAAAEEVGPAAHASDPVAAIAQAIASGAETLLEGAGSAGTLA
                     ALPSVTSEAFASFCEGCRLDGDPATQQEARAAFERAIELSPGFTLARKELGALLLDLG
                     LWEEAAAQYRTALEQEPNSGVLLTNLGRALAEMDDTAGAESVLRQAIGLGRDSMASAY
                     AYNNLGVAYLRAKRYEEALTAFERARRMLPRYAMAHANVARTYMLQDQFGRAADAAQR
                     AVEFANDRWALSYAHRLLGDIALRQNDLVAAVGHYHEAVAVRPEYAMVYNNLGVALKR
                     QGNIAGAEEAFSKAIALDNDHTAAAYAHTNRGNLCRDRGDRERAIAEYEAALRLKPDY
                     AAASDALRKLRGGE"
     gene            2425..2748
                     /locus_tag="PLQ54_06975"
     CDS             2425..2748
                     /locus_tag="PLQ54_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93040.1"
                     /translation="MKTIACRMVLVSALVALILGLGSLVCGCGIVVGEWGDPGWSWPD
                     DRWESDDPWDSHDDDYGYDGGSADYGDEDWGGDTSSSGSDSSSGDSGDDAIGEDDDGA
                     STAGL"
     gene            3246..>3537
                     /locus_tag="PLQ54_06980"
     CDS             3246..>3537
                     /locus_tag="PLQ54_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93041.1"
                     /translation="MTWSACVTPTVRALVLTALISCARAQQPTEPPQPAMPPSVTSPV
                     VGEDGRITFRLFAPAATAVRVSSGDMLGFGPGAGMEKNAEGIWEATVGPVGPG"
BASE COUNT          536 a         1200 c         1240 g          561 t
ORIGIN      
        1 ccagagccga ccgcctgcgc agcttggccc agatctggga cgagatgcgc gcgcgactgc
       61 gggccgacct gcccacggtc cccgagcacc ggcagcgcct cgaggcggcc ggcgcgccga
      121 ctgcgcccga gcaggttgga cagagccccg agagcgcccg ggcggccgta cgctacgggc
      181 ggtacctgcg agcgcggtac accgtgctcg atcttgccgc cgatctcgct gtcttggagg
      241 actgagagag acgccatgcc cggtgccacc gagctcgacg ccacgacccg cgcgaggaag
      301 atcgtcgccc ggctcatcca ggagtacccc gacgcgcggt gtgcgctgga ctacacgaac
      361 cccctggagc ttctgatcgc gaccatcctc tcggcccagt gcaccgatga gcgggtcaac
      421 atcgtcacca aggacttatt catcaaatat acccgggcca tcgactacgc cgtggcgacc
      481 caggagcagc tggagcaaga catcaagagc atcggcctct tccggcgcaa ggctcagtcg
      541 ctccgggcga cctgccaggc actcgtggag cggttcgacg gagaggtgcc cgacacgctc
      601 gaggagctgg tcacgcttcc cggtgtggcg cggaagacgg ccaatgtggt cctcggcaac
      661 gcattcggcg tgaccagtgg agtggtggtc gacacccacg tcttccgact ggcgcatcgc
      721 atgggcctca ccacgcagct ctatgcggac aaggtcgagg aagacctgat ggctctgtta
      781 ccgaagaaac ggtgggtaat ggcggctcac gtactgatct tccacggccg gcaggtgtgc
      841 acggcactcc ggccccattg cgccgagtgt gtggtggcgc ggtggtgtcc gcggttgctc
      901 gacgcgccga agcccaagta gcgcccgagc ggccagggag ccggggagtt gtccatacgt
      961 ctatacaagt ggagcgggcc cgggcccgct gagggggatc tgaggtgaag cgtggaatct
     1021 cgctctgcgt ggtggtggca ggtgtggttg cggcggctgg cgcctgggcc gctccctttg
     1081 tgtgtgtcct acccggcccc gatggtgacc ccgcgctttc tccgggcgct ctggccgtgg
     1141 ccgagtcgct cctgcgtcgt gacccggccc tcgcgatggc caatcgacgc caagcccagt
     1201 gggttctcgc ccaggcgggc gtcgcggcgg ggcggccgcc caccggcgcc caggccgagc
     1261 aggcgtgcga tcgtatcggc tgcagtgccg ctgtcctggt tagcctccgc gccgatggtg
     1321 ccgactacct cgcgcgggcc agcgttctcg tcgcgggcca ggcggcggcc gaagaggtcg
     1381 ggcctgccgc ccacgctagc gacccggttg ccgccatcgc ccaagccatc gcgtccggtg
     1441 ccgaaacgct tctcgagggc gccgggtcgg ccggcacact cgccgcgctg ccgtccgtta
     1501 cctcggaagc cttcgcgtcg ttctgcgagg gctgccggct cgatggcgat cccgcgacgc
     1561 aacaggaggc ccgggccgct ttcgagcgtg ccatcgagct gagcccgggg ttcacgctgg
     1621 cccggaagga gctgggggcg ctgctgctcg acctcgggct gtgggaggag gccgccgccc
     1681 agtaccggac ggcactggag caggaaccca atagtggcgt tctactcacg aatcttggcc
     1741 gtgcgctggc ggagatggat gacacggcgg gggccgagtc ggtgctgcgg caggccattg
     1801 gcttgggccg agactccatg gcatcggcct acgcgtacaa caacctggga gtggcctatc
     1861 tgagggccaa acggtatgag gaggccctca ccgccttcga gcgggcgcgg cggatgctgc
     1921 cccggtacgc catggcccat gccaacgtgg cgcggaccta catgctgcag gaccagttcg
     1981 gcagagctgc ggacgcggcc caacgggccg tcgagttcgc gaatgaccgc tgggcactga
     2041 gctatgcgca tcggttgctg ggcgacattg ccctgaggca gaacgacctc gtcgccgccg
     2101 tcggccacta ccacgaggct gtggccgtgc ggcccgagta tgccatggtg tacaataacc
     2161 tcggagtggc gctgaagcgg caggggaaca tcgccggggc cgaggaggcg ttctcaaagg
     2221 ccatcgcgct cgataatgac cacaccgccg ccgcctatgc ccacaccaac cggggtaatc
     2281 tgtgtcgtga ccgaggggac agggaacgcg ccatcgcgga gtacgaggcg gcactccggc
     2341 tgaagccgga ttacgcagcg gcgagcgacg cgctccgcaa gctgagaggg ggcgagtagg
     2401 gtccttcccg gggccctgaa ggccatgaag accattgcct gtcgcatggt tctcgtctca
     2461 gcgctggttg cgctgatcct cgggctcggc tccctggtct gcggttgtgg catcgtcgtc
     2521 ggtgagtggg gcgaccccgg ctggtcctgg cccgacgacc ggtgggagtc ggatgacccc
     2581 tgggactccc acgacgatga ctacggctac gacggcggca gcgctgacta tggggatgag
     2641 gactggggcg gcgacacgag ctcaagcggg tccgactcct catcgggcga ctctggcgac
     2701 gacgccatcg gtgaagacga cgacggcgca tcgacggcag gcctctgagg ccgaggccgc
     2761 ggacgacagc ccaatgttgt gatgactggg caactgagca tccttcctgc tgctggggcg
     2821 atgtccctgg tcgtgatact cgccgactgc gcgcacggtc actccttctg ctcttccttg
     2881 gtgcccgctg acctgtgcct cggtccctgt tgccgggcag agaactagac ccgtcacgcc
     2941 tcagccatcg tcgcccagag tggccaccgt gccgggggct cagcgtgttg cgcactgggg
     3001 gccaccgtcg agtggggctt cgtgggctgt cccgaggcca gccgtgtcac cgttctcagc
     3061 ggtgcggtgg cgcctgcccc ccttctgttc cactcgctgt ccgtggcggc cgcgtcggcg
     3121 catgcctcgg cgttcgggtt cgccccttcg gacgccctgc ccaccgacga ggcatcgccg
     3181 gcgcccgatg cgaaccctgg ccactgaaga tgatcggcca tcccacgagc caaggtgaac
     3241 ccgacatgac atggagcgcg tgcgtcaccc ccacggttcg agccctggtg ctcacggccc
     3301 tgatctcgtg cgcccgagcc cagcagccca ccgagccgcc gcagccggcc atgccgccct
     3361 cggtaacgtc cccggtggtg ggcgaggatg gccgcatcac cttccgcctc ttcgccccgg
     3421 ccgccacggc ggtgagggtc agcagcggcg acatgctggg cttcggcccc ggcgccggca
     3481 tggagaagaa cgccgagggc atctgggagg ccaccgtggg tcccgtcggc cccggcg
//
LOCUS       DAOSPL010000309         3532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26605_length_3532_cov_3.907391, whole genome shotgun sequence.
ACCESSION   DAOSPL010000309 DAOSPL010000000
VERSION     DAOSPL010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26605_length_3532_cov_3.907391"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="PLQ54_06985"
     CDS             complement(<1..1134)
                     /locus_tag="PLQ54_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93042.1"
                     /translation="MVYATIGAVIALAMPAATAPPYDIVVPVPAGEVTVREVWEGIVH
                     AEAQVVAGLPNPERFDEQAALPGDHPAEISLERALDAIAEPLNLIWFCHPSGLVLMYE
                     RPTQLTPVAWAGVHRDMLATLCTLWELVEALPSDEDRARFLAGEWVQPLSGDDPFARV
                     TARAVRNFGGGKVAPLDPGRPPAVWLCCLAVAMRRAGPSDPIAVQRPVLGKEDVIPPR
                     DRLEATGSPLAGGPRSFCTAAWRDRIAALARVQAVPYEPWDLSAFAPEGVSADRIVHG
                     QPVRGAEVAPTGAETVVRACTTPGSRSAVSALFDRYRVLISLREQPAQTVLGALMTVL
                     GAEMRGLDGTVVVAPADVMTDAWVTATANQVLHDILRPSARSLL"
     gene            complement(1139..2443)
                     /locus_tag="PLQ54_06990"
     CDS             complement(1139..2443)
                     /locus_tag="PLQ54_06990"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOS93043.1"
                     /translation="MRSWLLWLALLSGSSAARAGQAPSAPDVPFARIIYSSDIEGYLE
                     PCGCGGVNEGGIARRATLVERLRAEVPSVVISGGNFGGVAEAVSSMLSALSRMRYDYV
                     IPNSADLGILPTALDEAARLGLPAVPRSRPGAQVEPHQIQLGGLRIQLLAVGPIGPDE
                     PTLCSGFAAEACDLLLVVAEPMPAAARDRLARALGDQGFTSVLLASASPRAAQWLQAR
                     GHCLVPELPDRGRALVLIDCWPASDAAGFRISVRREPVSAALAPDAEVQRIATEYYDA
                     RRRASARGDRTAAVSPGGPSPTPSKPWLDPRVAYAPAEACGSCHPRSYERWRGSRHAR
                     ALQTLAAGDRVLPECLRCHSTPYALTGELPPPPYGAGDGVECAACHGDGVVHALTRRP
                     RFVTRCAPAERCMECHTPQDSPAFGGSYFDRLSCSAGKDGEG"
     gene            2762..>3532
                     /locus_tag="PLQ54_06995"
     CDS             2762..>3532
                     /locus_tag="PLQ54_06995"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOS93044.1"
                     /translation="MPFNDMLGRLAQPMQGRAGRISSNEQPEWNNANFDMTYIAPGET
                     FELPVIEGPAVINHIWFTSHAGHADTLDMLVLRIYWDGSHEPAVEVPMDSFFACGHGK
                     KLVVDSLPVQVSESGSLTCYWRMPFRQSARITVTNEHPSTTTGLYWQVDYLQLPVADD
                     QPYFYARYRQEYPCVTGRDYLICQAQGKGHYVGSVMSITMCQDGWFGEGDDFFYIDGE
                     EIPSLQGTGSEDYFNDAWGFRPRTTHSFGQPLWEGYAAG"
BASE COUNT          604 a         1275 c         1137 g          516 t
ORIGIN      
        1 caacaacgag cgtgccgacg gccgtaagat atcgtgcaag acctggttgg cggtggccgt
       61 tacccaggcg tcggtcatga cgtcggccgg agcgacgacg acggttccat ccagaccgcg
      121 catctccgcg ccgagcacgg tcatcaatgc ccccaggacg gtctgggccg gctgctcccg
      181 cagtgagatg agcacccgat aacggtcaaa cagcgcggag acggctgacc ggctgccagg
      241 agtcgtgcag gcgcggacca ccgtctcggc accggttgga gccacctcgg ctccccgcac
      301 tggttgcccg tggacgatcc gatcggcgct cacaccctcg ggagcgaaag cgctgaggtc
      361 ccacggctcg taaggcaccg cctggaccct tgcgagggcc gctatgcggt cacgccaagc
      421 cgcagtgcag aaggaccgcg gtccccccgc gagcggagag ccagtggcct cgagccgatc
      481 tctcggcggg atcacgtcct ccttgcccag cacgggacgc tgcacggcga tggggtcact
      541 gggaccggcc cgccgcatcg ccaccgcgag acagcacaac cacacggccg gtgggcgccc
      601 cgggtccagc ggcgcgacct tgccgccacc gaagttgcgg accgcgcgcg cggtgacccg
      661 ggcgaaaggg tcgtctcccg acagcggctg gacccactcc ccggccagga accgcgcccg
      721 atcctcgtcg ctcgggaggg cctccacgag ctcccagagc gtgcagagcg tcgcaagcat
      781 gtcccgatga acgccagccc aggcaacggg cgtcagttgc gtgggccttt cgtacatcaa
      841 tacgaggccc gaggggtgac agaaccagat caggttgagc ggctcggcga tggcatccag
      901 ggcccgctcc agcgatatct cggcaggatg atcccccggc agagccgcct gctcgtcaaa
      961 ccgctcggga ttgggcaggc cagccacgac ctgcgcctct gcatgcacga tgccttccca
     1021 gacctcgcgg actgtcactt caccagccgg gaccggtacc acgatgtcat agggtggcgc
     1081 ggtggccgcg ggcattgcga gcgcgatgac ggcaccgata gtggcgtaca ccatctctct
     1141 agccctcccc gtctttcccg gccgagcagc tcagccggtc gaagtaggat ccgccaaacg
     1201 ccgggctgtc ctgcggcgtg tggcactcca tgcagcgttc cgctggcgca cagcgcgtga
     1261 cgaagcgcgg ccggcgcgtg agcgcgtgca ccacgccgtc tccgtggcag gctgcgcact
     1321 ctacgccgtc gcccgcacca tacggcggcg gaggcaactc acctgtcagg gcgtatgggg
     1381 tcgagtggca ccgcaggcac tccggcagaa cgcgatctcc cgcggccagg gtctggagcg
     1441 cgcgggcgtg gcgcgatccg cgccagcgct catacgaacg cgggtggcag gagccgcacg
     1501 cctccgccgg cgcgtatgcc acacgaggat ccagccacgg cttgctgggt gtcgggcttg
     1561 ggccgcccgg tgagacggcg gccgtccggt cgcctcgcgc cgacgctctg cggcgcgcgt
     1621 cgtagtactc cgtggcgatc cgctgcacct ctgcatcggg ggccagcgcg gccgagacgg
     1681 gctcgcgccg taccgagatg cgaaagccgg ccgcgtcact cgcgggccag caatcaatca
     1741 gcacaagcgc acggcccctg tcggggagct caggcaccag gcagtggccc cgggcctgga
     1801 gccactgggc cgcccgggga gatgcgctcg ccaggagcac actggtgaac ccctggtcgc
     1861 cgagagcgcg tgcgaggcgg tcgcgcgcgg ctgccggcat tggctcggcc acgaccaata
     1921 gcaggtcaca ggcttctgcc gcaaacccgc tgcagagcgt tggttcgtcg ggcccgatgg
     1981 gcccgaccgc gagcaactgg atgcgcaggc cacctagctg gatctgatgc ggttcgacct
     2041 gggctccggg gcgactgcga gggaccgcgg gcaggccaag tcgggcggcc tcatccagag
     2101 cggtcgggag gatcccgagg tccgcgctgt tggggatgac gtagtcatag cgcatccgcg
     2161 agagggcgct gagcatggac gagacagcct cggcgacccc gccgaaattg ccgcccgata
     2221 tcacgaccga cggcacctcg gcgcggagcc gctccaccag cgtggctcgc cgcgcgatgc
     2281 cgccctcgtt cacgccaccg caaccacaag gctcgaggta accctcgatg tcggacgagt
     2341 aaatgatgcg tgcaaatggg acgtcaggcg ccgacggcgc ctgacccgcg cgagccgcgc
     2401 tcgatccgga aagcaacgcg agccatagta gccaactgcg caccggcaac gcccccgtcc
     2461 gggaacccat gccatccgcg cgacccggcc gcccgcaccc atccgcttca gccagctcgt
     2521 tccaccgttc cggtcctcac acacccctct gccctgccag accccgaata cccaagccag
     2581 aaactaagaa aacattgcat cattagtgta ttcgatgtgg tctccccccc cctcttggtg
     2641 tgcgagtttt ttttctgcaa ccgggccaag cagcgtcggt gccctcagcg caggtcctcg
     2701 cgtcccatgc ccggaacccg ccctctctgc ccctgggggg cacagggaag agggtgcagc
     2761 attgccattc aacgacatgc tcggccgcct cgcccagccc atgcagggcc gcgccggccg
     2821 catctccagc aacgagcagc ccgagtggaa caacgccaac ttcgacatga cgtacatcgc
     2881 gcccggcgag acgttcgagc tgccggtcat cgagggccca gcggtcatca accacatctg
     2941 gttcacctcg cacgccggtc acgccgacac cctcgacatg ctcgtgctcc gcatctactg
     3001 ggacggttcc cacgagccgg ccgtcgaagt gcccatggac agcttcttcg cttgcgggca
     3061 tggcaagaag ctcgtcgtcg attccctgcc cgttcaggtc tccgagtccg gttccctgac
     3121 ctgctactgg cgcatgccct tccgccagtc ggctcgaatc acggtgacga acgagcaccc
     3181 gtcgaccacc accggcctct actggcaggt cgactacctc caactcccgg tcgcggacga
     3241 ccagccctac ttctacgccc ggtaccggca ggagtaccct tgcgtcacgg ggcgcgacta
     3301 cctcatctgc caggcccaag gcaaggggca ctacgtcggc tccgtgatga gcatcaccat
     3361 gtgtcaagat ggctggttcg gcgagggcga cgacttcttc tacatcgacg gtgaagagat
     3421 cccctcactt cagggaaccg gttccgagga ctacttcaac gatgcctggg gcttccgccc
     3481 gcgcacgacg cactcgttcg gccagccgct gtgggagggc tacgccgccg gc
//
LOCUS       DAOSPL010000310         3530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26624_length_3530_cov_3.453525, whole genome shotgun sequence.
ACCESSION   DAOSPL010000310 DAOSPL010000000
VERSION     DAOSPL010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26624_length_3530_cov_3.453525"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="PLQ54_07000"
     CDS             <1..738
                     /locus_tag="PLQ54_07000"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS93045.1"
                     /translation="LSGALISLFTGGLGQAKRSVVIERKIHSQAERHELLEELLDMVE
                     QNPTDGEANFRLAFELDRACKYPEKAARHYDMAIRSLFGACDRRRVVEAYVAFWKAHS
                     FDELPPSTHLAVAACYETMGQYDAAIMVCRQILTHYPHADAEVESALMKIGVTCLEHT
                     GDHAMAKEALEALLARFPGSPWAAQAKALVGQLAGRSIAARSSSHRPFGSDEGLAGIR
                     PPGLPGVIPPGSDEGPRDRPANTDCSG"
     gene            827..2212
                     /locus_tag="PLQ54_07005"
     CDS             827..2212
                     /locus_tag="PLQ54_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOS93046.1"
                     /translation="MQLPTPAVAPPLDPRFRPAALANRALRQAAEAAGKSAPLAIALE
                     RGDGSISTYRTTAVAPGGPQHEANLEYAERIVKTLLWQRGGWRVIIGGPDGIGEHIRQ
                     VYSPSGPRAFDHEFMGGVYEKPFTVDVTDADRVPDTQEATAPLGRHLEGCRIGFDLGA
                     SDYKIAAVIDGESVFSDEIVWHPREQTDPDYHYEKIMEGLKMAMAHLPRVDAIGGSSA
                     GVYINNRVRVASLFRGIPKDLFDAKIAGMFVRIGQELGVPLEVINDGEVTALAGSMSL
                     EDNPVLGIAMGSSEAGGYVTPEGNVTGWLNELAFVPFDYQPEAAVDEWSGDRGVGSQY
                     LCQTGVIRLAPAAGIDLSAWATPAEKLKAVQDLMAAGDERVPPIYESIGSYLGYAIAH
                     YADFYELKHVLVLGRVTSGAGGQLIIDRANRVLGEEFPELAARITVSLPDERSRRVGQ
                     AIAAASLPAIG"
     gene            2224..3072
                     /locus_tag="PLQ54_07010"
     CDS             2224..3072
                     /locus_tag="PLQ54_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOS93047.1"
                     /translation="MKLHNATAEIFVPDGLPVDAALKRTTHMGIAAHQDDLEIMTYDG
                     ILKCFGRDDLWYCAITCTNGSGSPRDDLYADYTDEQMQVVRRKEQKKAAVVGEYGAQA
                     LLDYPSSGVKDPSNPALVEDLKAIIDVARPQVIYTHNLADKHDTHVGVALRTIRALRE
                     LPAEARPEVLYGCEVWRDLDWMADADKVGFDVSAHENLAAALLGVFDSQICGGKRYDL
                     ATMGRRRANATYFASHGVDEALSLAFGMDLTPLIRDDQMDPAALLDEHIQRFRAEVLE
                     RVRKFS"
     gene            3211..>3530
                     /locus_tag="PLQ54_07015"
     CDS             3211..>3530
                     /locus_tag="PLQ54_07015"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOS93048.1"
                     /translation="MVLIPWITAAYTPVATSFAVEILDRGVVCADAGAGGYQAFPDVV
                     RLADGDLLCVFYAGYDHISFPRESLPKGARVCACRSRDDGRTWEPPTIVADTPWDDRD
                     PSIV"
BASE COUNT          618 a         1212 c         1123 g          577 t
ORIGIN      
        1 ctctccggtg cactcataag tctcttcacc ggcggactcg gacaggcgaa gcggtctgtg
       61 gtcatcgagc gcaagatcca ttcgcaggcc gagcgccatg agctgctaga ggagctcctg
      121 gatatggtcg agcagaaccc cactgacggc gaggcgaact tccgcctggc cttcgagctg
      181 gatcgcgcgt gcaagtaccc tgagaaggca gctcggcatt acgacatggc catccggagc
      241 ctgtttggcg catgcgaccg ccggcgcgtt gtggaggcct acgtcgcgtt ctggaaggcc
      301 cactccttcg atgagcttcc gccgtccacc catctggccg tggccgcctg ctacgaaacc
      361 atgggccagt acgacgcggc gatcatggta tgccgacaga tcctgaccca ctatccccat
      421 gccgatgccg aggtcgagag cgccctcatg aagatcggtg tgacctgcct cgagcacacc
      481 ggcgaccacg ctatggcgaa ggaggcgttg gaagcgttac tcgcccgctt ccccggcagt
      541 ccctgggcag cgcaagccaa agccctcgtc ggccagctgg cgggacgctc gatcgccgcc
      601 cgaagctcga gtcaccggcc gttcggatcc gacgaaggac tcgcagggat ccgtcctccg
      661 ggacttcccg gagtgattcc gcccggatcc gacgaaggtc ctcgggatcg cccggcgaac
      721 actgactgct ccggctgact gtggcgggcc gtgtgaggcc cctgtgtggc atcgctgggt
      781 ctcgcaggca gcgcccggat tccgtgatga gagaagtgaa cgacccatgc aacttcccac
      841 cccagccgtg gctccccctc tggatccgcg ctttcgcccg gctgcgttgg ccaaccgcgc
      901 tctgcggcag gccgccgaag ccgcaggcaa gtccgcaccc ttggccatcg ccctcgagcg
      961 gggcgatgga tcgatctcca cgtaccgcac cacggccgtt gctccgggcg ggccccagca
     1021 cgaggccaat ctcgagtacg ccgagcgcat cgtcaagacc ctgctgtggc agcgtggtgg
     1081 ctggcgagtc atcatcggcg gacccgacgg catcggtgag cacatccgcc aggtctactc
     1141 cccttcgggc ccccgggcct ttgatcacga gttcatgggc ggcgtctacg agaagccctt
     1201 caccgtcgac gtcaccgacg ccgatcgggt gccggacacc caggaggcca cggcgcccct
     1261 gggccgtcac ctcgaggggt gccgcatcgg cttcgacctc ggcgccagcg actacaagat
     1321 cgccgcggtc attgatggcg agtccgtctt cagtgacgag atcgtttggc atccgcgcga
     1381 gcagaccgat cccgactacc actacgagaa gatcatggaa ggcctgaaga tggccatggc
     1441 ccacctgcct cgagtcgacg ccatcggagg cagctcggcg ggcgtctaca tcaacaaccg
     1501 cgtccgcgtg gcctcgttgt tccgcggcat acccaaggac cttttcgatg cgaagattgc
     1561 cggcatgttc gttcgcatcg gacaggagct cggcgtgcca ctcgaggtca tcaacgatgg
     1621 tgaggtgacg gccctggcgg gctcgatgtc cctcgaggac aacccggtgc tcggcatcgc
     1681 catgggctcc tcagaggcgg gcggctacgt cactcccgag gggaacgtaa ccggctggct
     1741 taacgaactc gccttcgtgc ccttcgacta ccagccggag gccgccgtag acgaatggtc
     1801 cggggaccgc ggcgtgggca gccagtacct gtgccagacg ggtgtaatcc ggctggcccc
     1861 cgccgccggc atcgacctct cggcctgggc cacccccgcc gagaagctca aggccgtcca
     1921 ggacctgatg gcggcgggcg acgagcgtgt cccgccgatc tacgagtcca ttggttccta
     1981 cctgggatac gccatcgccc actacgcgga cttctacgaa ctcaagcatg tgctagtcct
     2041 ggggcgagtc acctcaggtg cgggcggtca gctcatcatc gaccgcgcca atcgggtgtt
     2101 gggtgaggag ttcccggagc tggctgcccg catcacggtc agccttccgg acgagcggag
     2161 ccggcgcgtc ggtcaagcca tcgccgcggc cagcctgccg gccattggtt aaggggagcc
     2221 accatgaagc tacacaacgc aaccgccgag atcttcgtgc ccgacggact gccggtcgac
     2281 gcggcgctca agcgcaccac ccatatgggc atcgcggcgc atcaggatga cctcgagatc
     2341 atgacgtacg acggcatcct caagtgcttc gggcgcgacg acctgtggta ctgcgcgatc
     2401 acctgcacca acggcagcgg gagcccgcgc gatgacctct acgcggacta caccgacgag
     2461 cagatgcaag tcgtgcgccg caaggagcag aagaaggcag ctgtggtcgg cgagtacggt
     2521 gcccaggcac tgcttgacta tccgagttca ggggtcaagg atccctccaa ccccgcactg
     2581 gtcgaggacc tgaaggccat catcgatgtg gcccgacccc aggtcatcta cacccacaac
     2641 ctggccgaca agcatgatac ccacgtgggt gtggccctgc ggacgatccg cgccctgcgc
     2701 gaactacccg ccgaggcccg gcctgaggtc ctctatggct gtgaggtctg gcgcgatctc
     2761 gactggatgg ccgatgccga caaggtcggc ttcgacgtca gcgcccacga gaacctcgcc
     2821 gcggcgctgc tgggggtgtt cgactcccag atctgcggcg ggaagcgcta tgacctggcc
     2881 accatgggcc gccgtcgcgc caatgcgacc tacttcgcgt cgcacggtgt cgacgaggct
     2941 ctgagcctgg ccttcgggat ggatctcacg ccgctcatcc gcgatgacca gatggacccc
     3001 gcggccctcc tggatgaaca catccagcgg ttccgtgccg aggtactcga gcgggtcagg
     3061 aagttcagct aggcaagcga gagcccgaat accctgtgcg catcaaccag cggccggatg
     3121 gaaggggccc gcagtgcgac ggacaccatc cggcacgctg tgcgcggccg cggaaccggg
     3181 cctggagcgc ctccctggag ggcgaacacc atggttctga tcccctggat cacggcggca
     3241 tacaccccag ttgccacatc ttttgccgtc gagatactgg accgaggcgt ggtctgtgcc
     3301 gacgcggggg ctgggggcta ccaggcgttc cccgacgtgg tccgcctggc ggatggcgac
     3361 ctgctgtgcg tcttctacgc cggctatgac cacatctcat ttccacgcga gagcctaccc
     3421 aagggcgcac gcgtctgtgc ctgccgctcg cgtgacgacg ggcgcacctg ggaaccaccc
     3481 accatcgtcg ccgacacgcc atgggacgac cgcgatccat ccatcgtcca
//
LOCUS       DAOSPL010000311         3524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26682_length_3524_cov_4.081580, whole genome shotgun sequence.
ACCESSION   DAOSPL010000311 DAOSPL010000000
VERSION     DAOSPL010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26682_length_3524_cov_4.081580"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="PLQ54_07020"
     CDS             <1..1167
                     /locus_tag="PLQ54_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93049.1"
                     /translation="APKQGPAPPKTPRIQLARTARPETDQLLRDLVHEVSERSARSLA
                     AREMDRREREERAGAAFGVSLQKRGSPLAELARDESVLAPSESPDAEEITLGRAKRHG
                     PVTPVRARDNELLRVVGEMADSAVFSLDNIIRGSRGYSVLAADMDAEHTVHVEGGPAL
                     STAMADLVRGSRDATMACARAVESTDARERVGLSSAFGGLEIRFDSYASVMPADEDTS
                     GEDIVPVAASGEAGSVRDLMGQVVAYTTSAGPADDSEEKISASTEGPSISFAELIGRA
                     VRKIYELSDEERRNLTDGLDGALAGLDDAGTPAKRARAMLRCFDHWRRLLQVVDELSS
                     KGMSDPPPLGLLFAAMAEYVVTQEEEHAADGSAARTKAAAALRAYRDRIASKEA"
     gene            1170..1931
                     /gene="kdsB"
                     /locus_tag="PLQ54_07025"
     CDS             1170..1931
                     /gene="kdsB"
                     /locus_tag="PLQ54_07025"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012939023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOS93050.1"
                     /translation="MAPLTAAGIIPARFASTRLPGKALLDICGKPMIQWVQEGACATG
                     VLDRVIVATDDERIARVVRSYGGEVVLTRPDHPTGTDRIAEVAADIDLDVVVNIQGDE
                     PFIEADMIRQLVEPFGADAQLAMTTLRAPIAPEELHDPSTVKVVVDQRGYALYFSRAT
                     IPHQRSESPEDRCPVWKHLGLYGYRRDFLLRYPSLPQTPLQRAEQLEQLRALENGYRI
                     WVGLTQHESIGVDTESDLRRARELMARRLAEEGRR"
     gene            1928..2749
                     /gene="kdsA"
                     /locus_tag="PLQ54_07030"
     CDS             1928..2749
                     /gene="kdsA"
                     /locus_tag="PLQ54_07030"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012629562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOS93051.1"
                     /translation="MNPVRVGPVAIGPGHPLALLAGPCVIESVDHCLRMADALREVAQ
                     RLAMPFVFKASYDKANRTSVDSYRGPGLEAGLSVLARVRTECGVPTLTDVHSPPEATA
                     AGAVVDLLQIPAFLCRQTDLLVAAGATGRPVNVKKGQFVSPETMVQAARKVEAGGTGG
                     VLLCERGSVFGYGNLVVDMRGLVIMRELGYPVVFDATHSVQLPSAGGTVSGGDRRFVA
                     HLSRAATAVGIDALFLEVHDDPDHALCDGPNMVALAELPEMLEAVLRVRESVTSR"
     gene            2896..>3524
                     /locus_tag="PLQ54_07035"
     CDS             2896..>3524
                     /locus_tag="PLQ54_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOS93052.1"
                     /translation="MFKRLARVVRAMFGWLVRRAEDPELILRQHIDDLRSRIPEFNRQ
                     TAEVVKLEKMLAMQAERLRQKAADLEKQVVAAVKLGPSRKEIAKSLISQLETTKLDLA
                     ETEQQLAQAKQNSEQVKRARLSYERKIQQEIQESMRQISRAKRAEMQRQMASLMMSFE
                     VGDDNQVLDQMTQKIDEDLARAEARTEIAQESVGAQMVDISIDAMDQEA"
BASE COUNT          599 a         1219 c         1156 g          550 t
ORIGIN      
        1 gccccgaagc aaggacctgc cccgcccaag accccacgaa tccagctggc tcgcacggcc
       61 cggcccgaga ccgaccagct cctgcgtgac ctggtgcatg aggtgtccga gcgcagtgcc
      121 cggagcctcg cggcgcggga aatggaccgc cgcgaacgcg aagagcgagc cggcgccgcc
      181 ttcggtgtct cactccagaa gcgcgggtcg ccactggccg agttggcgcg tgatgaatcc
      241 gtgctcgctc cgtccgagag cccggatgcc gaagagatca ccctcggccg tgccaagcgc
      301 cacggccctg tgacccccgt ccgggcccgc gacaatgagc tgctccgagt cgttggcgag
      361 atggccgata gcgccgtgtt ctctctcgac aacatcatcc gcggctctcg tggctacagc
      421 gtcctggcgg cggacatgga tgccgaacac acggtccatg tcgagggcgg acccgcgctc
      481 tcgacggcca tggccgacct ggtacggggc tcccgcgacg ccacgatggc ctgcgcgcgt
      541 gccgtcgaga gcaccgacgc acgcgagcgc gtgggtctca gcagtgcctt cggcgggctc
      601 gagatccggt tcgactccta cgcctcggtg atgccggccg acgaagacac gtccggagag
      661 gacatcgtgc ccgtggccgc ttctggcgaa gccggttccg ttcgcgacct gatgggccag
      721 gtcgtcgcgt acaccacctc ggcgggcccc gcagatgact ccgaagagaa gatctcggcc
      781 agcaccgagg gcccatcgat cagcttcgcc gaactgatcg ggcgcgccgt ccggaagatc
      841 tacgagctct ccgacgagga gcgccggaac ctcaccgatg gcctggatgg ggctctggcc
      901 ggcctcgacg acgccggcac ccccgccaag cgcgcccgtg ccatgctccg gtgcttcgac
      961 cactggcgac gtctgctgca ggttgtggac gagttgagct cgaagggaat gtccgatccg
     1021 ccgcctctcg ggctcctctt cgcggccatg gccgagtatg tggtcaccca ggaggaggag
     1081 cacgcggcgg acgggtcggc tgcccgaacc aaagccgctg ccgcattgcg ggcctatcgc
     1141 gaccgcatcg ccagcaagga ggcatagaca tcgctcccct caccgctgcc ggcatcatcc
     1201 cggcccgctt cgcatcgacc cggctccccg gcaaggccct cctcgatatc tgcgggaagc
     1261 ccatgattca gtgggtgcag gaaggcgcct gcgccaccgg agtcctcgac cgggtcatcg
     1321 tggccacgga cgacgagcga atcgcccgcg tggtgcgttc gtacggaggc gaggtggtgc
     1381 tcacgcggcc cgaccatccc accggcaccg accgcatcgc cgaagtcgct gcggacatcg
     1441 acttggatgt ggtcgtcaac atccagggcg atgagccctt catcgaggcc gacatgatcc
     1501 ggcaactcgt cgagcccttc ggcgccgatg cccagttggc gatgacgact ctgcgcgcgc
     1561 ccattgcgcc cgaggagctc catgatccgt ccacggtcaa agtggtcgtc gaccagcgcg
     1621 gctatgcgct ctatttctcc cgagccacca taccgcacca gcgttccgag tctcccgagg
     1681 accgctgccc cgtctggaag cacctgggcc tctacggcta ccgacgtgac ttcctcctgc
     1741 gctacccgtc gctcccccag acacccctgc agcgcgccga gcagctcgag cagctccggg
     1801 ccctcgagaa cggctatcgc atctgggtcg gcctcaccca gcacgaatcc atcggagtcg
     1861 acaccgagag cgacctgcgg cgggcccggg agctcatggc ccgccgtctg gctgaggagg
     1921 ggcggcgatg aacccggtcc gggtcggccc cgtcgccatc ggtcccgggc acccgctcgc
     1981 cctcctggcg gggccctgcg tcatcgagtc cgtcgatcac tgcctgcgga tggcggacgc
     2041 gctgcgcgag gtcgcccagc gcctggcgat gccgttcgtt ttcaaagcat cgtacgacaa
     2101 agcgaaccga acttcagtcg actcctaccg cggaccgggc ctcgaggcgg gcctatcggt
     2161 gttggcccgg gtccgcaccg agtgtggagt tccaacactt accgatgtgc atagtccacc
     2221 ggaagccacg gccgccggcg ccgtcgtcga cctcctccag atcccggcat tcctgtgccg
     2281 acagaccgac ctgctcgtgg ccgctggtgc caccggtcgt cccgtcaatg tgaagaaagg
     2341 gcagttcgtg agccccgaga ccatggtcca ggccgcccgc aaagtggaag caggaggaac
     2401 gggaggcgtt ctcctgtgcg agcgtggctc ggtcttcggc tatggaaacc tggtcgtcga
     2461 catgcgcggg ctggtgatca tgagagagct ggggtacccg gtggtgttcg acgccaccca
     2521 ctccgtgcag ctccccagcg cgggcggcac cgtctcgggc ggcgaccggc gcttcgtggc
     2581 gcatttgtcc cgcgcggcta cggcggtagg aatcgacgcc ctcttcctgg aagttcatga
     2641 tgatccggat catgccctgt gcgacgggcc aaacatggtg gcgctggcgg agctgcccga
     2701 gatgctcgag gcagtgctga gggtccgcga gagcgtcacc tcccgctagt gcggttttgg
     2761 atccaccggg accgtcgact tcatcggact cgggccgggg tcgatccgaa actggacggt
     2821 ggggtcggcg tctcgtctga ctggggctcg cggccgcgag cggtctagcc ccatcccgat
     2881 tacggaggca cacgcatgtt caagcgtctc gccagggtcg tgcgcgccat gtttgggtgg
     2941 ttggtccgcc gcgccgagga cccggagctc atcctccgcc agcacatcga cgatctgcga
     3001 tcgcgaatcc ccgagttcaa ccgccagacc gctgaggtgg tgaagcttga gaagatgctc
     3061 gcgatgcagg ccgagcgcct ccgtcagaag gccgcagacc tcgagaagca ggtcgtggcc
     3121 gcggtcaagc tcggcccctc gcgcaaggag attgccaagt cgctgatctc gcagctcgag
     3181 accaccaagc tcgatctggc cgagaccgag cagcaactgg cccaggccaa gcaaaactcg
     3241 gagcaggtga agcgcgcccg tctgtcttac gagcgcaaga tccagcagga gatccaggag
     3301 tccatgcgcc agatctcccg cgccaagcgc gccgagatgc agcggcagat ggcgagtctg
     3361 atgatgagtt tcgaggtcgg tgacgacaac caggtcctcg accaaatgac ccagaagatc
     3421 gacgaggacc tggcccgcgc cgaggcgcgc accgagatcg cgcaggagag cgttggtgcc
     3481 cagatggtcg acatcagcat cgatgccatg gaccaagagg caga
//
LOCUS       DAOSPL010000312         3523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26696_length_3523_cov_2.806228, whole genome shotgun sequence.
ACCESSION   DAOSPL010000312 DAOSPL010000000
VERSION     DAOSPL010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26696_length_3523_cov_2.806228"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PLQ54_07040"
     CDS             complement(<1..632)
                     /locus_tag="PLQ54_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93053.1"
                     /translation="MYPRREFLKRVAAGAAGIAAGVTAGCPAGSREEADGATRAAPTP
                     VASAAPGGPGPKVVMATHSRATLEGEVQPEAVEKLVDAAVVALTGEKTAQAAWNKLFS
                     SDESVGVKVNGLGGPFISTSRHLTAAVVSRLLQVGVKAERITIWDQNPEFLRNCGLIV
                     DSKEWGTNIVPVNIEWTDAPVEAGKFRGRITTIVTKRVDCFVNLPIMKDHG"
     gene            complement(686..1540)
                     /locus_tag="PLQ54_07045"
     CDS             complement(686..1540)
                     /locus_tag="PLQ54_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS93054.1"
                     /translation="MRVGRREFIGRAVGALGAITLGPAGAEPGSPPIGMCDWSLGQMA
                     SVGAINLAAEIGLDGAEVSLCTPAMPEWLQRAEVREQYLASAGEKGIRIPSIALGVLN
                     EVPLKSEERAARWVADAIPAAQALGAKVILLAFFGRGELSMEDAAGVDRVVGVLKDMA
                     PDARKAGVVLGLESYLTAEEHLVLLDRVGHDSVQVYYDCKNAAHRRHDPVTEIRQLGR
                     GRLCQVHLKNGDLLLSQRRDVDFAAVVQALKDVGYDGWIVLETSAPNSLVADTRANMA
                     YVRELWSA"
     gene            1692..>3523
                     /locus_tag="PLQ54_07050"
     CDS             1692..>3523
                     /locus_tag="PLQ54_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93055.1"
                     /translation="MNFGLGSRVRLASLPCLVAVLVTGLQVAGHAESFGDSARAVTRL
                     CERFSHWDENRDGTVEVDRLSPLGVPRAATTALRGTVLVLVEDRLLSSGPELPDLREE
                     LDGYAVQLAADGWNVLCVAARVYAGPRHQDGRTLLALRRFFTAVKHRVPSFSGAVLVG
                     SFPEAYLVRQYNWRQHNPFTINAGTSREKVFSEPVHLIRSRAEPVATRCELVLADLDG
                     RWDRAYFEKPTALPSAIAVYGTEERPAGGPTGDYELGSDTFEDFFFVNDGRYSLTMRQ
                     DGQIDFRALPTENRECSPRDLRLPNLMARPDICVSRINPRGVALRPKTDVRGTAGEGL
                     LGADGLPQSVTFASKEERPRWTAVWEPDPALERRLIIEFLRRDRRYRRGEFAAACKPA
                     SISTEFGSSLEEFRTAFPAWRDFSEEGYDIDGREATVVAAIEWLRRPALLRAFKAHSS
                     PWGSDFFPTEDVTQIERAVGGRAWSWQEGEAVLTPGLKHSTSRLDFAVYRSLYENHAL
                     PDAANLFIHTGCESIAPPGAHNRPYSAPEYGSWQGAEALMFYCNGLALIGRAKVFYDE
                     PREFAAQLGKGRTWGEAWRHYFDIEARAASEDEVGGGIGRKRAY"
BASE COUNT          557 a         1243 c         1102 g          621 t
ORIGIN      
        1 ccgtggtctt tcatgatcgg caggttcacg aagcagtcca cgcgcttcgt gacgatggtg
       61 gtgatccgcc cccggaactt gccggcctcc actggggcgt ccgtccactc gatgttgacg
      121 ggcacgatgt tcgtgcccca ctccttcgag tcgacaatga ggccgcaatt cctcaggaac
      181 tcggggttct ggtcccagat ggtgatccgc tccgccttca cccccacctg gagcaggcga
      241 gagaccactg ccgcagtcaa gtgccggctc gtcgagatga acggtccgcc caggccgttc
      301 accttcaccc cgacggactc atccgagctg aacagcttgt tccaggccgc ctgggcagtc
      361 ttctcaccag tcagagcgac cacggcggcg tccacgagct tctcgacggc ctccggctgc
      421 acctcaccct cgagggtcgc cctgctgtgc gtggccatga cgaccttggg cccgggcccg
      481 cccggggcag cgctggctac tggagttggg gccgcccgcg tcgcgccgtc agcctcttcc
      541 ctggagcccg ccgggcaccc cgcggtgacg cctgccgcaa tgcccgcagc tccggccgcg
      601 actctcttca ggaactcccg acgggggtac acgcatctgt ctcccatcat ctacctcccg
      661 gggcggcgtg tgccgctttc cgatctcagg cactccacag ctcgcgtaca taggccatgt
      721 tggcccgcgt gtccgcgact aggctattgg gggcggaggt ctccagcacg atccacccgt
      781 cgtatccgac atccttcaac gcctggacaa ccgcggcgaa atccacgtct cgcctctgag
      841 atagcagaag gtctccgttc ttgaggtgca cctggcacag ccgcccgcgg cccagttggc
      901 ggatctccgt aacagggtcg tgtcgccggt gcgccgcgtt cttgcagtca tagtagacct
      961 gcaccgagtc atgcccgacg cgatcgagaa gcaccaggtg ttcctcggcg gtgaggtagc
     1021 tctcgagccc cagcaccacc ccggctttgc gtgcgtccgg tgccatgtcc ttgagcacgc
     1081 ccaccacgcg gtcaacgccc gccgcatcct ccatcgagag ttcaccgcgt ccaaagaacg
     1141 ccagtaggat gaccttggca cccagtgcct gggccgccgg gatcgcgtcg gccacccacc
     1201 gcgccgcgcg ctcctctgac ttgagcggca cttcattcag cacacccagg gcaatggagg
     1261 ggatgcgaat acccttctct ccagcagagg ccaggtactg ctctcgcacc tccgcgcgct
     1321 ggagccactc cggcatcgct ggtgtgcaca ggctcacctc ggcgccatcc agcccgatct
     1381 ccgccgccag gttgatggcg ccgacggacg ccatttgacc caggctccag tcgcacatcc
     1441 cgatgggcgg cgagcccggc tcggctccgg cagggccaag cgtgatggca cccagagctc
     1501 ccaccgccct cccgatgaac tcacgtcggc ccacgcgcat gccacacctc gcattccggg
     1561 agttcccact ggtcctcccc tattcgccgt catgtgccgg tacgccttgc ttgtgccgcc
     1621 gggcaggcca gcgcttcccc atcgcgaaca tggcccctga ctgctagtgt ccgtatcgtg
     1681 tggagggtgc gatgaacttc gggttgggtt cgcgagtccg cctggcatca ctaccgtgtc
     1741 tcgtcgccgt actggtcacg ggcttgcagg tcgctggcca tgccgagagc ttcggtgatt
     1801 cagcgcgtgc ggtcactcgg ctctgtgagc gtttcagcca ctgggatgag aaccgggatg
     1861 ggacggtcga agtcgaccgt ctctcgccgc tcggcgtgcc ccgcgctgcc acgaccgcgc
     1921 tgcggggcac cgtgctggtc ttggtcgagg accgcctcct ctcaagcggt cccgagctcc
     1981 cggatttgcg tgaggagctc gacgggtacg ccgtgcagct cgcggccgac ggctggaacg
     2041 tcctgtgcgt ggccgctcgc gtctacgccg ggccgaggca ccaggatggg cggaccctac
     2101 tggcgctccg gcgtttcttc acagcggtga agcaccgcgt gcccagcttc tctggagccg
     2161 tgctggtggg cagcttcccg gaagcctacc tcgttcggca gtacaactgg cggcagcaca
     2221 accccttcac cattaacgcg ggtacgtccc gcgagaaggt cttctcggag cccgtccacc
     2281 tcatccgatc gcgagcggaa ccggtggcga cgcgctgcga gctggtgctc gccgacctgg
     2341 acggccgttg ggatcgagcc tacttcgaga agcccactgc tcttccatcg gccatcgccg
     2401 tctacggcac cgaggaacgc cccgcgggag gccccacagg ggactatgag ttggggagcg
     2461 acacattcga ggatttcttc ttcgtcaacg acggccgcta ctcgctgacg atgaggcagg
     2521 acgggcagat cgacttccgt gccctaccca ccgagaaccg cgagtgctcg ccgcgggacc
     2581 tcagactgcc taacctcatg gcgcgcccgg acatttgtgt gtcgcggatc aacccgaggg
     2641 gtgtcgccct gcgcccgaag accgatgtcc gcgggaccgc cggcgagggt ctcctcggcg
     2701 ccgacgggct gcctcagagc gtcaccttcg cctccaagga ggagagaccc aggtggaccg
     2761 ccgtctggga gcccgacccg gctctcgaac gacggctcat catcgagttc cttcggcggg
     2821 accgacgcta tcgcaggggc gagttcgcgg ctgcgtgcaa acccgcgtcg atctcgaccg
     2881 agttcggctc atcgctcgag gagttcagaa ccgcctttcc ggcatggcgt gatttcagcg
     2941 aggagggcta cgacatcgat ggccgcgagg ccacggtcgt ggctgccatc gagtggctca
     3001 gacgcccggc ccttctgcgg gcattcaagg cccatagcag cccgtggggc agtgacttct
     3061 tccccaccga ggatgtcacc cagatcgagc gggctgttgg cggccgggct tggagttggc
     3121 aggagggcga ggcggtgctg accccggggc tgaagcactc caccagccgg ctcgacttcg
     3181 ccgtctaccg cagcctctat gagaaccacg ccctacccga cgcggccaac ctcttcatcc
     3241 acaccgggtg tgagtccatc gctccaccgg gagcccacaa ccgaccctac tcggcacctg
     3301 agtacggcag ttggcagggg gctgaggccc tgatgttcta ctgcaacggt ctggcgctca
     3361 tcggccgcgc gaaggtgttc tacgacgagc ccagggagtt cgcggcccag ctcggcaagg
     3421 gacgaacctg gggtgaagca tggcgtcact acttcgatat agaagcccga gccgcttccg
     3481 aggacgaggt cgggggcggc attggccgca aacgcgccta ttt
//
LOCUS       DAOSPL010000313         3521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26715_length_3521_cov_2.665897, whole genome shotgun sequence.
ACCESSION   DAOSPL010000313 DAOSPL010000000
VERSION     DAOSPL010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26715_length_3521_cov_2.665897"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="PLQ54_07055"
     CDS             complement(<1..959)
                     /locus_tag="PLQ54_07055"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOS93056.1"
                     /translation="MRCPRAVALTAAAALAFGLLALGCQKPEAEPAPTPAPGPTAKAA
                     TDQPGEGMKIALVLKTMTNPFFQRMRDGAQKKAAELGAEIVSSATSTESSFDDQAKYV
                     QDMIQSKVDAILIAPEGSKEIVPVLVEAKKAGIIVINLDNRVDAEAAKAAGLELDAYV
                     GADNAEGGYLAGKYLAEKLGGKGVVTMIEGIPSADNAIARREGFEKAMAENPGIRVLK
                     PFQSGQWEQDPAYNIMTNLLSTHPDLNGLFCANDMMALGAMRAIAGAHKTGKITVVSY
                     DNLEEVQPALRKGELAATIEQHPDEMGSVGVEYAVRIKSGKPLT"
     gene            complement(974..1936)
                     /locus_tag="PLQ54_07060"
     CDS             complement(974..1936)
                     /locus_tag="PLQ54_07060"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS93057.1"
                     /translation="MQLLWRRLKPLLGSRRSVQALGLVIALVAVMVYFRLKVDQFWGP
                     DNLLDLLRQSSINTLLAVGVTFVILTAGIDLSVGSMLCLSTIVLALALRQGAPPAVGI
                     GIGMLAGTLCGALNGSLVVAGRLSPFIATLGTLWALRGLARWICNDRTILIASEPFMA
                     MGTARPLGVPMPVVVACIAVIVAEVILQRTVFGRCIFAVGGSEEASWVSGVRVGWIKT
                     SVYAISGLTAGIAAVLSSMKLGTGSPTVGVAYELDAIAAVVLGGTSLMGGEGSAVGTF
                     VGAVFIQALRKGMSMTGVEDYRQNIAIGVAIVIGALVDRSMRRR"
     gene            complement(2042..2860)
                     /locus_tag="PLQ54_07065"
     CDS             complement(2042..2860)
                     /locus_tag="PLQ54_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93058.1"
                     /translation="MSETSEPLTSSLASCRAPRALSRAKVPLAAAALLLTGSLLPAPA
                     GSDSAPSPKAVVGYVNVFRLQTNARIYGESLAEMDRRRSAAETRKALEREWRFLTGDK
                     AARAVSLAMKGTARTPEETQEFEALRQEHSDLDLEWYTLKSKDYLDSRELGRLRDLQA
                     IADARREELRQINAALDAELEQARQAMTARGNEKLIDALAATVKKHDLDVVLSSHVRS
                     WQPSAAGDSLVQVWENVVLYGGQDVTDDVLATMNAAGPAPAPGENAAAERGSRQ"
     gene            3226..>3521
                     /locus_tag="PLQ54_07070"
     CDS             3226..>3521
                     /locus_tag="PLQ54_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93059.1"
                     /translation="MCRWFRAKCVLGALAVWVMAGASPGQPAAEGGPARPGLHARADG
                     TLTRSGRLYRGIGVNYFDAFYRHLKDPADTTYDAGFAALAAAQIPFARLMGCGF"
BASE COUNT          516 a         1152 c         1216 g          637 t
ORIGIN      
        1 gtgaggggct ttccgctctt gatgcggacc gcgtattcga ctccgaccga gcccatctcg
       61 tcggggtgct gctcgatggt ggctgccagt tcacctttcc tcagtgctgg ctggacctcc
      121 tcgagattgt catagctcac cacggtgatc ttgccggtct tgtgggctcc ggcaatggcc
      181 cgcatcgctc ccagggccat catgtcgttg gcgcagaaga gcccattgag gtccggatgg
      241 gtgctgagca ggttggtcat gatgttgtac gcggggtcct gctcccactg gccggattgg
      301 aagggcttga ggacgcgaat accggggttc tcggccatgg ccttctcgaa cccctcccgg
      361 cgggcgatgg cgttgtcggc cgaggggatc ccctcgatca tggtcaccac gccctttccc
      421 ccgagcttct ccgccaggta cttgccggcg agatagcccc cttcggcgtt gtcggcgccg
      481 acataggcgt cgagctcgag cccggccgcc ttcgccgcct cggcatccac gcggttgtcg
      541 aggttgatga cgatgatgcc ggccttcttg gcctcgacca gcacgggaac gatctccttg
      601 gatccttcgg gcgcgatcag gatggcgtcg accttgctct ggatcatgtc ctgcacgtac
      661 ttggcttggt cgtcgaagct gctctcggtg cttgtggctg acgagacgat ctccgctccc
      721 agctcggccg ccttcttctg cgcgccatcg cgcatccgct ggaagaaggg gttggtcatc
      781 gtcttgagga ccagcgcgat cttcatccct tcgcccggct ggtcggtcgc ggccttggcc
      841 gtggggccgg gggcgggcgt gggtgctggt tcggcttcgg gcttctggca gcccagagcg
      901 aggaggccga acgccaaggc cgcggcggcc gtgagtgcga cggcacgagg gcaacgcatg
      961 tgactacaca cccctatcgt cttcgcatgg atcgatccac cagggcgcca atgacgatgg
     1021 cgaccccgat ggcgatgttc tgccggtagt cttcgacgcc ggtcatgctc atgcccttgc
     1081 ggagggcctg gatgaacacg gcacccacga aggtgccgac ggccgagcct tcgccgccca
     1141 tcaggctcgt gcctcccaag accacggcgg cgatggcgtc gagctcgtag gccaccccca
     1201 ccgtcggtga gccggtgccg agtttcatcg atgacagcac ggccgcgatg cccgcggtca
     1261 ggccgctgat ggcgtagacg ctggtcttga tccatccgac acgcacgccc gagacccacg
     1321 aggcctcctc gctgccgccc acggcgaaga tgcatcgccc aaacacggta cgctgtagga
     1381 tgacctcggc cacgatcacg gcgatgcagg ccaccacgac gggcatcggc acgcccagcg
     1441 gtcgtgccgt acccatggcc atgaagggtt cacttgcgat gaggatggtg cggtcgttac
     1501 agatccaacg ggcgaggccg cggagcgccc agagcgtgcc gagggtggcg atgaacggtg
     1561 agagccttcc ggcgaccacg aggcttccgt tgagggcgcc gcagagcgtc ccagccagca
     1621 tcccgatgcc gatgcccacg gctggcggcg ctccctggcg gagcgccagc gccagcacga
     1681 tggtcgagag acacagcatc gagccgaccg agaggtcgat gcctgccgtc aggatgacga
     1741 aggtgacgcc cacggccagc agggtgttga tggagctctg gcggagtagg tccagcaggt
     1801 tgtccggacc ccagaactgg tcgactttca gccgaaagta gaccatcacg gcaaccaggg
     1861 cgatgacgag ccccagtgct tggacggacc ggcgggagcc caggagcggt ttgagccttc
     1921 gccacagaag ctgcatggat cggcagacca cctcagccgt gctcgggggc cgggtcgacc
     1981 ccggtgtggc gtctcttttc ggtccacccc gaggggcagt cctgctccgg cgtcagaggc
     2041 cctactgtct cgatcctcgc tctgcagcgg cgttctcgcc gggggccggc gcgggaccgg
     2101 cggcgttcat cgtggcgagc acatcatcgg tgacgtcttg gccgccgtag aggaccacgt
     2161 tctcccagac ctgcacgagc gagtcgccgg cggccgaggg ttgccacgag cgcacatggc
     2221 tgctcagcac cacatcgagg tcatgcttct tgacggtcgc agcaagtgca tcgatgagct
     2281 tctcgttgcc ccgagcggtc atggcttggc gcgcttgttc cagctcggca tcgagcgccg
     2341 cattgatctg gcgcagctcc tcacgtcggg cgtcggcgat ggcttggagg tcgcgaagcc
     2401 ggccgagctc tcgcgagtcg agatagtcct tggacttgag cgtgtaccac tcgagatcga
     2461 ggtcactgtg ttcctgccga agcgcttcga actcctgcgt ttcctccggt gtgcgcgcgg
     2521 tacccttcat ggcgagggac acggcgcggg cggccttgtc gcccgtgagg aaccgccact
     2581 cccgttcgag tgccttgcgc gtctcggccg cgctgcggcg ccggtccatc tcggccaggc
     2641 tctcaccata gatccgcgca ttggtctgca gtcggaacac attcacgtac ccgacgaccg
     2701 cttttggtga tggtgcgctg tccgagcctg ccggcgccgg gagcagactt ccggtcagga
     2761 gcagggccgc tgccgcgagg ggaaccttgg cccggctcag ggcccgcgga gcacggcacg
     2821 aggcgaggga ggaggtgagg ggctcgctgg tctcagacat gaggtgtcct ccgggcatga
     2881 tggttacctc ttggaccttc gacgcgcacg gaatgttgcc tgtgtcgaac cgtggatgcg
     2941 ggtgcggcgt cgcgcggcac ccgggggacg aaggatcccg gccggctcgg cgctgcggtc
     3001 aggacgccgc gcgccgggat ccctcgtcgc cgtccaggcc gcagccgatc gcgccgcagc
     3061 atcggccctc ggtctggtct cccacgaagc acaccggggc gtgtgggctc tcccgcgcat
     3121 ggtcgcgagg ccgagggcca gcggcactct tgcgctcccc aggtggcgtg ggttgccaca
     3181 cggaatgtgg tcagccggtc gtgcaacgtt ggaggtgtct gcgcgatgtg ccgatggttt
     3241 cgggccaagt gtgtgctggg tgcgttggcc gtgtgggtga tggcgggcgc gagtccgggc
     3301 cagcccgccg ccgagggcgg tcctgcgcgg ccgggcttgc acgcgcgagc cgacggtacc
     3361 ctcacccgca gtgggcggct ctaccgtggg atcggggtga actacttcga tgccttctac
     3421 cggcacctga aggaccccgc ggacacgacc tacgatgccg gcttcgctgc tctcgcggcc
     3481 gcgcagatcc ccttcgcgcg actgatgggg tgcggtttct g
//
LOCUS       DAOSPL010000314         3516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26754_length_3516_cov_4.651546, whole genome shotgun sequence.
ACCESSION   DAOSPL010000314 DAOSPL010000000
VERSION     DAOSPL010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26754_length_3516_cov_4.651546"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="PLQ54_07075"
     CDS             complement(<1..1251)
                     /locus_tag="PLQ54_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOS93060.1"
                     /translation="MTASDLETLNDGGLNRGLKDRHIQLIALGGIIGSCFFLGTGEVV
                     GQVGPGAFLAYVLGGVIIFCVTISLGELAVALPISGSFVTYASEFISRSWAAGVGWTY
                     WINWVAYIPAECVAAGIIMHNFVPAVSEWIWAVAFGLLITVINLLNVKFFGEMEFWLA
                     LTKIFGLVLFVALALLIWVGLIGNRGSDGFIGFRYITAGGKGLFPLGLFTLLNTMVLL
                     LVNYQGSEIIGLTAGEAKEPAKTIPRAVRNVVIRICTLYIVPVFTLVLIMPWTEAGVS
                     ESVFAAALQRYGLGWAGTLFSIVALTAALSCANSGMYGTIRSLWSLSREGMAPKFLTK
                     LNKHAVPGNATIATMVGIWAFLAGSYFFSASAVFVALLSVSGFTGTMCWLSLCWSQIN
                     FRRRLRTAGYSAQELQYRAPGYPWT"
     gene            2005..3093
                     /locus_tag="PLQ54_07080"
     CDS             2005..3093
                     /locus_tag="PLQ54_07080"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HOS93061.1"
                     /translation="MTVLGTRPDGLGLLEALCALPGVSGQEAAVTAALKQHLCGVAEV
                     EQDRLGNAWAYAGGPDTRGVALIAHADQIGFIVTDVDDRGFVCMERVGEAFGDLLPGR
                     ELVVHASSGPVHGVVGRTPRDLVVPEELDKVADVRDQWLDIGARTREEALEVLRIGDP
                     VTFVPRFLRLKNGLLASPALDDRAGLYVVLRAFEEWVGAGGQRGDTGHENRVAVVATA
                     REETGFLGARALARLLACEVAIVVDVIFASDDPGVSSRRAGGRVELGKGPVIARGTAS
                     AEHLVRLAEGVAEEKHIPFQLRAAAGLAGTDADEMLTAHGCSVLSVSIPLRYMHSAME
                     VVAEADLEACQGLLVGVIGAWERRRGGA"
BASE COUNT          577 a         1107 c         1254 g          578 t
ORIGIN      
        1 cgtccaggga tagcccgggg cgcggtactg gagttcctgc gcggagtagc cggcggtccg
       61 caggcggcgg cggaagttga tctgcgacca gcagagcgag agccaacaca tcgtcccggt
      121 gaagccgctc accgagagca gggcgacgaa gaccgcgctc gccgagaaga agtagctccc
      181 ggcaaggaag gcccagatgc cgaccatggt ggcgatcgtc gcgttaccgg gcacggcatg
      241 cttgttgagc ttggtgagga acttcggcgc catgccctcg cgcgacagcg accacaggga
      301 gcggatcgtc ccgtacatgc cgctgttggc gcaggagagg gcggcggtga gggcgacgat
      361 cgagaacagg gtcccggccc agccgaggcc gtagcgctgc agggcggcgg cgaagaccga
      421 ctcggacacg cccgcctcgg tccacggcat gatgaggacg agtgtgaaca cggggacgat
      481 gtagagcgtg cagatgcgga tgacgacgtt gcgcaccgcc ctggggatcg tcttcgccgg
      541 ctccttggcc tcaccggcgg tcagaccgat gatctccgag ccctggtagt tcacgagcag
      601 caggaccatg gtgttgagga gggtgaacag gccgaggggg aagagaccct tgcctccggc
      661 ggtgatgtag cggaaaccga tgaagccgtc cgagcccctg ttgccgatga ggccgaccca
      721 gatgaggagg gcgagggcga cgaagaggac gaggccgaag atcttggtga gggcgagcca
      781 gaactccatc tcgccgaaga acttgacgtt cagcaggttg atgacggtga tgaggagtcc
      841 gaaagcgacc gcccagatcc actcgctcac cgcgggcacg aagttgtgca tgatgatgcc
      901 ggcggcgacg cactcggcgg ggatgtaggc cacccagttg atccagtatg tccaccccac
      961 gccggcggcc caggagcgcg agatgaactc cgaggcatag gtcacgaacg agccgctgat
     1021 cggcagggcc acggcgagct cgccgagtga gatggtgaca cagaagatga tgacgccgcc
     1081 gaggacgtag gcgaggaacg cccccggacc cacctggccg accacttctc ccgtcccgag
     1141 gaagaaacac gaaccgatga tccccccaag ggcgatgagc tggatgtgcc ggtctttgag
     1201 gcctcgattc agccccccgt cattcagggt ctcgagatca cttgccgtca gggtgtcacc
     1261 aacgccttgc tgtgccaccg tcccttcgac gtcctggtgt gccactgctg ccctcctctc
     1321 gcgtcttacc ggccgctgtc tgcggggctg gaccgctgga ccgggccagc ccacttcgta
     1381 catcacgcga agtgttgcat tgctgattcc tacctcgtca atcgccagga tgtcctattc
     1441 ggcaggtatt cggctccatg atggcaatat cggcagaacg cgcatgtgcg tctgggcatc
     1501 cttcacgctg agacgagcgg gttgcggccc cgggcgcgcg aggctctggg caggcgccga
     1561 cttcgccatc ttcgacgtgc agtgcggtcc cgggcgcgcg aggctcggcg tcttgtatcg
     1621 gtcgccggtc gctatgcgtc gctactcatg ggtctgcgac ggccactggg gtgtcgcttg
     1681 ccggcgaggt ggggcaacgg gctacctgga ccggccggca ccatccagtc ctcctcaagg
     1741 gggcgagcgt cgcctggcag cctcgacgcg gacgggtagc acagctccga agcgcagagc
     1801 ccctgcggga ggaggcagag gctgcgaccg gccggaacaa cggcctgcgc tctacgctaa
     1861 gatgcagcgg atcatcatga gcgatgccgc cgtggcattg ccgcgtcagc cggtcgagag
     1921 cggcacgaat cgggaggacg gcggaaggtt cgacaccgac gcccgcccgc ttttcacacc
     1981 tgtcggccac cggaaggggt cggattgacg gttctgggga ctcggcctga cggcctcgga
     2041 cttcttgagg cgctgtgcgc tctgccgggc gttagcggac aggaagccgc cgtgacggcg
     2101 gcgctcaagc agcacctgtg cggtgtcgcc gaggtcgagc aggatcggct cggcaatgct
     2161 tgggcgtacg ccggcggtcc ggacacacgc ggggtggcgc tcatcgccca tgccgaccag
     2221 atcggcttca tcgtcacgga cgtcgacgac cgcggcttcg tctgtatgga gcgtgtcggc
     2281 gaggcgttcg gtgaccttct gcccggaagg gagctggtgg tgcacgcctc gtccggcccg
     2341 gtgcacggtg tcgtcggccg gacgccgagg gaccttgtcg ttcctgagga gctcgacaag
     2401 gtcgcggacg tccgcgacca atggctcgac atcggggctc gcacgaggga ggaggccctt
     2461 gaggtgctcc gcatcggtga ccctgtcacc ttcgtgcccc ggttcttgcg tctgaagaac
     2521 gggctcctcg cttcgcctgc actcgacgat cgtgccggcc tgtacgttgt ccttcgggct
     2581 ttcgaggaat gggtcggcgc cggcgggcag agaggtgaca ccggtcacga gaaccgcgtc
     2641 gcggtggtgg cgactgcgag ggaagagacc ggattcctgg gtgcgcgcgc gctcgcgaga
     2701 ttgttggcgt gcgaggtggc catcgtcgtc gatgtgatct ttgccagcga cgatcccggc
     2761 gtgtcctcgc ggcgagccgg cggccgggtg gagctcggca aggggccggt gatcgcgcgg
     2821 ggaacggcga gcgccgagca tctcgtgcgg ctggccgagg gcgtggcaga agagaagcac
     2881 atcccctttc agttgcgtgc ggcggccggt ctcgctggga cggacgccga cgagatgctc
     2941 accgcgcacg gatgttcggt cctctcggtg agcatcccgc tgcgctacat gcactccgcc
     3001 atggaggtcg tggccgaagc cgacctggag gcctgtcagg gactgcttgt gggggtcatt
     3061 ggcgcctggg aacgacgccg cggcggcgcc tgacccggga tcccgcgtaa gtgcgcgaga
     3121 gcatcgggtc ggaagatcga cgcggccatc gcgtgccccc tgtcgaccca gtcaccgccg
     3181 accccacttg ccgacctgac aggtcgggcg ccccctccct tcgacgtcac gcgagcggga
     3241 ggcgcggggc ggaagacgac acttgccgat aagcactcgg gtgacacctg cgggcaagtc
     3301 gcaggcgcgg ccggcctcac ctgtcggctg acccgcccgt gccgctcgct tgtccgcttc
     3361 ggcttccgct gccgtgcccc tcgagcgatc cgccgcgtgt ccgctgtcgt gccctctcaa
     3421 caagggatcc ggcctgatct gcgggtagta gcccggatgc ggtatcttcg gcgtctttgc
     3481 cattgacatt ggcgtttctt cgggatataa gcgcgg
//
LOCUS       DAOSPL010000315         3507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26855_length_3507_cov_3.277520, whole genome shotgun sequence.
ACCESSION   DAOSPL010000315 DAOSPL010000000
VERSION     DAOSPL010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26855_length_3507_cov_3.277520"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLQ54_07085"
     CDS             complement(<1..273)
                     /locus_tag="PLQ54_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93062.1"
                     /translation="MEPGPDLRGDPLPHEDIRVAFGEIEYVPRRSLRAEGRSTFRLVA
                     GILALVFGTFTSMLALSTILPIVLSTLSMAPIPPSLVAVALTFVGVS"
     gene            complement(357..893)
                     /locus_tag="PLQ54_07090"
     CDS             complement(357..893)
                     /locus_tag="PLQ54_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOS93063.1"
                     /translation="MTASNLRSAHGGESMAHMRYKVWADKAEKDGFPNVARLFRAVSH
                     AEHVHSWNHFSRLRVEPGDHTVIAGAGFGLASTSENLAGACGGEEFEVTEMYPAYIAI
                     AEAQGEAGAAQSMKFAFEAEKVHLEMYCKAKESVDAGQDPALGVIQVCDMCGHTLEGD
                     APDKCPVCGASKDKFIAF"
     gene            1107..2540
                     /gene="melA"
                     /locus_tag="PLQ54_07095"
     CDS             1107..2540
                     /gene="melA"
                     /locus_tag="PLQ54_07095"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOS93064.1"
                     /translation="MPKIAMIGAGSLVFTRTLMNDLLATPALADSEFCLMSRTAPKLQ
                     RMEAFIKRVIAENGLGASVWSTLDRREAIKDADYVIVMIQVGGMDAFAVDYQIPLKYG
                     VDQCIGDTLGPGGIFRALRTIPPVVDIARDMDGLCPRALLLNYANPMAMVCWSLGRES
                     RISFVGLCHGVQTTLDLISGYVGVPKDQIRYVAAGINHMAWFLRLEKDGQDLYPVLRA
                     NIEKPGYYVNEKVRCEVMRQFGYFMTESTGHLSEYLPWFRSSQRALELYCDEPAFGGE
                     SGAYYKWGKTVAEKFEQTDPLAHETPTLEPRSAEYCSYIIEAIETDQVFRLNGNVRND
                     GYITNLPTGCCVEVPCYTDGTGIHPTHVGALPPQLAALNQSNVTVQGLAVEAALSGDP
                     ELIVAAVALDPLTSAVLTPREARDMAAEMLEAEKPWLPQFEGKSIQPTPIISIPADVQ
                     RADVPLDPALAIGKRFVKLMEIATQQG"
     gene            2634..>3507
                     /locus_tag="PLQ54_07100"
     CDS             2634..>3507
                     /locus_tag="PLQ54_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93065.1"
                     /translation="MTRTIGQVVIGIGLASGIAFPADLLPNSGFGDPGDGLAAGWMLQ
                     TDVGAATASRVDEDGPFLRLERADRGQADLRPSAGQVPVRATQAYLLTARVRTSNVAE
                     GSHSAELQWFGPAGFLSRHIGTAHVAGRWVTVAIGPVRPPDGATSVAVLLRCYDPGVY
                     DFDDVQLVEVEPMPRNLIANPGFESDADGDGAPDGWTLAPAGVIWDAENPLTGARSMR
                     LQRAAADAPVASIRQDGIRVDAGRAYELSAGARSDVFGRELRLAVEWTKGPQVLETAE
                     FRDQTFQTWQRKTLR"
BASE COUNT          583 a         1239 c         1115 g          570 t
ORIGIN      
        1 cgacacgccc acgaacgtca gcgccacagc cacgaggctg ggcgggatgg gggccatact
       61 gagcgtggac agcacgatcg ggaggatggt gctgagggcc aacatgctgg tgaacgtgcc
      121 gaagaccagg gccaggatgc ctgccacgag ccggaacgtc gaccggccct ccgcgcggag
      181 cgaccggcgg ggcacgtact cgatctcgcc gaacgccaca cggatgtcct catgggggag
      241 ggggtcaccg cggagatcgg ggccgggctc catcggacac ccttccgtcg caacacgaga
      301 gggccggagc cggtcggacg ctccagccct ccctcgtctc tgttcggcgc ggacgcctag
      361 aacgcgatga acttgtcctt actagctcca cagaccgggc acttgtccgg agcgtcgccc
      421 tcgagcgtgt gcccgcacat gtcgcacact tgtatgacac cgagggccgg gtcttgcccc
      481 gcgtcgacgg actccttggc cttgcagtac atctcgaggt gcaccttctc agcctcgaag
      541 gcgaacttca tgctctgcgc ggcacccgcc tcgccctgag cctcggcgat cgcgatatag
      601 gcggggtaca tctcggtaac ttcgaactcc tcgccgccac acgcaccagc taggttctcc
      661 gacgtgctcg ccagcccgaa accggcaccg gcgatcaccg tatggtcgcc cggctccacg
      721 cggaggcggc tgaagtggtt ccaagagtgg acgtgttcag cgtgtgacac ggctctaaag
      781 agccgcgcga cgttcgggaa cccgtccttc tccgccttgt cagcccatac tttgtagcgc
      841 atgtgagcca tggattcgcc gccatgggca ctgcgaagat tcgaagcggt cattgcacgc
      901 accggagtga cctccctgag ccgtcgccac tgcggcgacc gcgttccctg ctaaggtact
      961 gcactgcgcc cggccagtgc tgcagcgcca tgctgttcca cgactgccga gggcatccct
     1021 ctcgccgccc gaggttcgag catgccgcac accgaatgct actcgggcga cgcctgggcg
     1081 cgccaccttc tcctgggggg accaacatgc cgaagatcgc gatgattggg gcagggagcc
     1141 tcgtgttcac ccgcacgctg atgaacgacc tactcgcgac gcccgccctg gcggacagcg
     1201 agttctgtct catgagccgc acggcgccga agctgcagcg catggaagcc ttcatcaagc
     1261 gcgtcatcgc cgagaacggg ctcggcgcca gcgtctggtc caccctcgac cgccgcgagg
     1321 ccatcaagga cgccgactac gtcatcgtga tgatccaggt cggcgggatg gatgcctttg
     1381 cggtggatta ccagatcccc ctcaagtatg gggtggacca gtgcatcggc gacaccctcg
     1441 ggccgggcgg catcttccgc gcactgcgga ccatcccgcc cgtggtcgac atcgctcgcg
     1501 atatggatgg gctgtgccct cgtgcactgc tgctcaacta cgccaacccg atggccatgg
     1561 tctgttggag cctcgggcgc gagagccgga tttcgttcgt cggcctgtgc cacggcgtcc
     1621 agacgaccct cgacctgatc tcgggctacg tgggcgtgcc gaaggaccag atccgctacg
     1681 tcgcagccgg gatcaaccac atggcatggt tcctccgtct tgagaaggat gggcaggacc
     1741 tctatccggt tctgcgcgcc aacatcgaga agcccggcta ctacgtcaac gagaaggtcc
     1801 gctgcgaggt catgcggcag ttcgggtact tcatgaccga gagcaccggg catctttcgg
     1861 agtacctgcc ctggttccgg agcagccagc gggcgctgga gctctactgc gacgagcccg
     1921 ccttcggagg cgagtccggc gcctactaca agtggggcaa gacagtggcc gagaagttcg
     1981 agcagaccga cccactggcc cacgagaccc ccacgctcga gccgcgcagt gccgagtatt
     2041 gctcgtacat catcgaagcc attgagaccg atcaggtgtt ccggctcaac ggtaacgtcc
     2101 gcaacgatgg ctatatcacc aacctgccca cgggctgctg cgtcgaggtc ccgtgctaca
     2161 ccgacgggac cggcatccac ccgacccacg tcggtgcgct gcctccccaa ctggcggccc
     2221 tcaaccagag caacgtgact gtccaggggc ttgccgttga ggccgcgctc tcaggtgatc
     2281 ccgagctcat cgtcgccgcg gtggctctcg atccgctcac ctcggctgtg ctcaccccgc
     2341 gagaggcgcg cgatatggct gccgagatgc tcgaggccga gaagccatgg ctgccgcagt
     2401 tcgagggcaa gtccatccag cccacgccca tcatcagcat ccccgctgac gttcagcgcg
     2461 ccgatgtccc cctcgacccg gccctggcga tcggcaagcg cttcgtgaag ctgatggaga
     2521 tcgcgacaca gcaggggtag ccggccgacg cccgcgctgg gggatcccgg cgcccccgat
     2581 cagctggagt ggcgactggg gccgcgctgg cccccatgag ggagtgactc gccatgaccc
     2641 gcaccatcgg ccaagtcgtg atcggcatcg gcctggcgtc gggtatcgcc ttccccgccg
     2701 acctgctgcc gaactcgggc ttcggtgacc ccggcgacgg cctggccgcc ggttggatgc
     2761 tccagaccga tgtcggcgcc gcaaccgcga gtcgggtcga cgaggacggc ccattcctgc
     2821 gcctcgagcg tgccgacagg ggccaggccg acctccggcc gtcggccggg caggtccccg
     2881 tgcgagcaac gcaggcctat ctgctcaccg ctcgcgtccg cacatccaac gtggccgagg
     2941 gctcccactc ggctgagctt cagtggttcg ggcccgcggg gttcctgagc cgccacatcg
     3001 gcaccgcaca cgtggccggc cgctgggtca ccgtcgccat tggcccggtc cggccgcccg
     3061 acggcgccac atccgtggcg gtactgctcc gatgctacga tcccggcgtc tacgacttcg
     3121 atgacgtcca gctagttgag gtggaaccca tgccacgcaa cctcatcgcg aaccccggtt
     3181 tcgagtccga cgccgacggc gatggcgcgc ccgatggctg gaccctcgcc cccgctggcg
     3241 tcatatggga cgccgagaac ccgttaaccg gcgcacgttc tatgcggctg cagcgcgccg
     3301 ccgctgacgc cccggtcgcc tccatccggc aagacggcat ccgcgtcgac gccggtcgcg
     3361 cctatgagct ctccgccggc gctaggtcgg acgtcttcgg gcgcgagttg cgtctggctg
     3421 tcgagtggac caagggaccg caggtgctcg aaaccgccga gttccgcgac cagactttcc
     3481 agacatggca gcgcaagacc ctccgcg
//
LOCUS       DAOSPL010000316         3505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26873_length_3505_cov_3.597971, whole genome shotgun sequence.
ACCESSION   DAOSPL010000316 DAOSPL010000000
VERSION     DAOSPL010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26873_length_3505_cov_3.597971"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PLQ54_07105"
     CDS             complement(<1..396)
                     /locus_tag="PLQ54_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS93066.1"
                     /translation="MSRIRWEPVPYDVVVVGAGHAGCEAALAAARLGARTLLLTLSLD
                     RVAHMACNCSIGGPGKSHLVREIDALGGQMARTTDATLTHIRVLNTRKGPAVQSLRAQ
                     VDKRAYEATMKLALERQPGRARRRRRRLPG"
     gene            complement(435..701)
                     /locus_tag="PLQ54_07110"
     CDS             complement(435..701)
                     /locus_tag="PLQ54_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93067.1"
                     /translation="MSDRTDSAVQESLDLEVAPDIVVLVKRLTELMECIRRAVGESQD
                     ARERSHRMGLEEITRELDMGRLRLTRARQILEQYLVNGPKGDRP"
     gene            complement(866..938)
                     /locus_tag="PLQ54_07115"
     tRNA            complement(866..938)
                     /locus_tag="PLQ54_07115"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(903..905),aa:Thr,seq:cgt)
     gene            1316..3247
                     /locus_tag="PLQ54_07120"
     CDS             1316..3247
                     /locus_tag="PLQ54_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93068.1"
                     /translation="MPVLLFMLCCALSPTGSRNLVANPSFEDAFGTPLWPTAWTQLDG
                     GPIELREKGGHTGRRYVHFSDGRTDQGSALESRHAPAIPGATYRMQAFVRTHNECQPG
                     IYIQFHDGRGARISEVHARFPGPWDGWMPFEVRAVAPPEAVEVSALLYAFVGDVGDFD
                     FDDVELVLESWPTSGGVRPAPPYATSRQLESVAGRWASLSSAMPGGKSMVDIGSRLEL
                     FVDSFLVDGLAGSAERRLHHPAPQEAVFYFDRPWEGSSSAYVTVMQDTDRIRLYYRGS
                     DLKGTHELTCYAESEDGIHFERPSLGLFEFEGSTANNIIWTGTGVHNFAPFKDTNPEC
                     AADQQYKAVAGGPLIALVSEDGVHWRQLVEDPIITEGAFDSQNLAFYDNLRGEYVCFL
                     RDFANGVRAIRRSTSRDFVHWTAPEWINHGTAPPEHLYTNATVPYFRAPHIYLAFPKR
                     FLPERTKDPSHGDPGVSDGVLMSSRDGMHWERWIEGFLRPGPDPRNWTQRSNMICWGL
                     IPSSEREISIYWSEHYCHDSAHLRRGTIRTDGFVSVHAGVPGGEMLTRPFTFTGNSLV
                     VNYATSAAGSVRFELCNEPGEPIEGFAMADSELLFGDEIAHTVAWRGGSDVASLAGRP
                     VRLRVRLEDADLYSFRFAP"
BASE COUNT          572 a         1123 c         1189 g          621 t
ORIGIN      
        1 gcccggtaga cggcgtcgac gtcgccgtgc tcggccgggc tggcgctcga gcgccagttt
       61 catggtggcc tcgtaagccc tcttgtcgac ctgggcacga agcgactgga cggcgggtcc
      121 ctttcgagtg ttgagcacac ggatgtgtgt gagagtggca tcagtggtcc gtgccatctg
      181 gccgcccagg gcatcgatct cccgcacgag gtggctcttt ccgggcccgc cgatgctgca
      241 gttgcaggcc atatgggcca cgcggtcgag gctcagtgtg agcagcaacg tgcgggcccc
      301 aagccgcgcg gcggccaggg ccgcctcgca accggcatgg ccggcgccga ccaccaccac
      361 gtcatacggg acaggctccc agcggatgcg actcatggct gcttccggcg cccagccgtg
      421 cgggatggct cccgctaggg tcggtcgccc ttcggaccgt tgacgaggta ctgctcgagg
      481 atttggcggg cgcgcgtcag tcggaggcga cccatgtcga gttcgcgggt gatctcctcg
      541 aggcccatgc ggtgagaacg ctcgcgagca tcctgggact ccccgacggc gcgccgaatg
      601 cactccatga gctcagtgag gcgcttgacc aggaccacga tgtcgggcgc gacctccagg
      661 tcgaggctct cttgcactgc gctgtcggtt cggtcagaca tggcgagagt gggccctgct
      721 cctgtgggta tccatctggc tcgcgggcga tgttcgcgcc tcgccgaggg aatcctacct
      781 tcggattgcg cccagtgcga agggatgcca gaacggaaac ggagcacgcc ggcgcagcgc
      841 gtgctccctc gatggctccg atcggagccg acgacccgac tcgaacgggt aaccggctga
      901 ttacgattca gctgctctgc caattgagct acgtcggccg gaacaaactc agcatagaag
      961 gataccgcac gccgtgctga cctgtcaacg cccgctcggg gtcatttcgt tggcccgccg
     1021 ggccgtgagc ctcaccagcg cagctggcct tgggccggat tgactcccac cagcccggag
     1081 cgggttcgcc atgggcagtc gctctgaggg ttcccggggc gccctgctca cgaccatctc
     1141 gtcgtcgggg acgtcggacg ggaacgttga ccatgactca gtggagtcag ccgacggacc
     1201 tcgacgaggt agctcgcgcc caccgatggg ttcgggtaac agcgcgagga gacggagggg
     1261 acgccagccg ccacggggaa ggggtgtctc gacgccatgc ggggaggatg ggcccatgcc
     1321 ggtcctgttg ttcatgctct gctgcgccct gagccccacc ggatcgcgga atctggtggc
     1381 gaatccttca tttgaggatg cgttcgggac cccactgtgg ccgacagcct ggacacagct
     1441 cgatggcgga cccattgagt tgcgcgagaa gggtggccac acgggtcggc ggtacgttca
     1501 cttttcggac ggcaggaccg accagggctc tgccctggag tcgcgccatg ccccagccat
     1561 tcccggggcc acgtaccgga tgcaggcgtt cgtgcgcacg cacaacgaat gccaacccgg
     1621 tatctacatc cagttccacg atggccgggg cgcacgtatc agcgaggtgc atgcgcgctt
     1681 tccggggccg tgggacggct ggatgccctt cgaggtccgg gcagtggcgc cgccggaggc
     1741 cgtcgaggtt tcggcgctgc tctacgcctt cgtgggtgat gtgggtgact tcgacttcga
     1801 cgacgtggaa ctcgtcctcg agtcgtggcc cacgagcggg ggagtgaggc cagcgccgcc
     1861 ctatgccacc tcgcgccagc tggagtcggt tgcggggcgt tgggcgtccc tatcgagcgc
     1921 catgcctgga gggaagtcca tggtggacat cggatcgaga ctcgagctgt tcgtggactc
     1981 attcctggtg gatggactcg ccggcagcgc cgagcgccgg ctgcatcatc cggcgccgca
     2041 ggaggcagtg ttctacttcg acaggccgtg ggaggggtcg agcagcgcct atgtgacggt
     2101 gatgcaggac acggaccgga tccggcttta ctatcggggc tcagacctaa aaggcactca
     2161 cgaactaacc tgctatgccg agagcgagga tggcattcac ttcgagcggc cgagtctggg
     2221 gctgttcgag ttcgagggat ccacggcgaa caacatcatc tggactggga cgggagtgca
     2281 caacttcgct ccgttcaagg acacaaaccc cgagtgcgcg gccgatcagc agtacaaggc
     2341 ggtagccggg gggccgctga tcgctctcgt ttcggaggac ggggtacatt ggcgccaact
     2401 cgttgaggat cccatcatca ccgaaggtgc cttcgactcg cagaacctag ccttctatga
     2461 caacctccgc ggtgagtacg tatgcttctt gcgcgacttc gccaacgggg tccgcgccat
     2521 ccgccgctcg acatcacgcg acttcgttca ctggaccgct cccgagtgga tcaaccatgg
     2581 gactgcaccg ccggagcacc tctacaccaa cgcgacggtg ccgtacttcc gggcgccgca
     2641 catctatctc gccttcccca agcgattcct gcccgagcgc accaaggacc cgagtcacgg
     2701 tgaccccggg gtgtcggatg gcgtcctgat gagcagtcgc gacgggatgc actgggagcg
     2761 gtggatcgag ggtttcctgc ggcccgggcc ggatccgagg aactggacac agcgcagcaa
     2821 catgatctgc tgggggctga taccgtcttc ggagcgggag atctcgatct actggagcga
     2881 gcattactgc cacgactcgg cccaccttcg gcgcgggacc attcgcactg acggattcgt
     2941 ctcggtgcac gcgggagtgc cgggcggcga gatgctaaca cggccgttca cgttcaccgg
     3001 aaactcactc gtggtgaact acgcaacctc ggcggccggg tcggtacgat tcgagctgtg
     3061 caacgagccc ggtgaaccca tcgagggctt cgccatggct gactccgagc tgctgttcgg
     3121 ggacgagatc gcgcacaccg tcgcctggcg aggcggctcg gacgtggctt cactggcggg
     3181 gaggccggtc cggctgcggg tgcgactcga ggatgccgac ctgtactcgt tccgctttgc
     3241 cccgtaagcc gagcggatcg accggccccc gcacatattc taaaatcggt gaaggccctt
     3301 gacacggagg cgtcgaatgg gccggacgcc atgcgcttcg tacgtcgaca cgccctggcg
     3361 tgttcctttg ccattctggc tactctgcta tggggaacgg accctgcgct gccgcagacc
     3421 ggctccgtcc cgtcgtggct gtccgcgtcg cgaccggctc ccgccttcgg tctggcggcc
     3481 ctggccggca tcgatgcggc ctgcg
//
LOCUS       DAOSPL010000317         3503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26901_length_3503_cov_3.099188, whole genome shotgun sequence.
ACCESSION   DAOSPL010000317 DAOSPL010000000
VERSION     DAOSPL010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26901_length_3503_cov_3.099188"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PLQ54_07125"
     CDS             <1..325
                     /locus_tag="PLQ54_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93069.1"
                     /translation="KAAGSVELLKESIALCRPGGTVSVVAFYERIVEQFDIDGLVFGD
                     VTLRAVAGSLGMYKPILRLMACGMLDLTPLITGRYPLLNAHKAMADIRDRNDTRVKIM
                     MEGEG"
     gene            complement(335..1909)
                     /locus_tag="PLQ54_07130"
     CDS             complement(335..1909)
                     /locus_tag="PLQ54_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOS93070.1"
                     /translation="MRPLSTHRGVWRPCAVLLGVGLAGLASGCSTGNLPLLPLPAASP
                     GAETALGHVGARERLAAHRQRILTMTGAVVTEPAARQTVSFELVVGGSADVSTRGTTP
                     QKGGVSLTSTDPGDSGSGRPTWSGNRVTGWVRVNNNTGATMFATKARITAITGGATCD
                     DSEWNYGSIAHGSSSDWLPWVFTDDADHVFTFTVQVTWLTMSGMPRAAWVKDNGEGTW
                     IYVSALDGLPAVPITAVPTTYYDCPALSADGTRMAMLRDTLMGLDVFIGSADGTSTWA
                     NVSNDDVNVEIDVWLSGDGTRVAWSAEDTYTNESDVYRSDDAGFLVENISNDPARDDF
                     WPSLSNDGRVVVWTKYDPTARTWQVYRYASGTITNLSSNPSVADSSAVVSADGLNVVW
                     QRYAGGDNEVMRWSNGTVTNISNDASSSDESPSMSADGTVIVWAKRAASGDYDIYAKK
                     GSAAAVNMSADPTRSDRHPSLSADGSTVVWQKLGVGAGGDSEVYAAPLSGGAFNVSSD
                     PSGYDSVLSARGKGPR"
     gene            complement(1948..>3503)
                     /locus_tag="PLQ54_07135"
     CDS             complement(1948..>3503)
                     /locus_tag="PLQ54_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93071.1"
                     /translation="RRAWFAALFVPVALLFACGCGLQSDSPAGAPDIPIVGPPSAEVA
                     RLHLEEKLRALREHDRNIAVPPEEETSSAQPGRHAATFELTFGDVSSRGTTPPNAAGV
                     SLTSTDPGNDGSGIPEWAGDVVTGWVRVNNGTGATMYGVRARVDSVSGGATYDDSAWN
                     YGTIEAGASSGWLPWSFTDADHAFSFVVSVTWLTLSGAPTALWSKQNSAGEWLYASVL
                     SGSDTPVPLTNDPTHTYRYPALSADGSTMALVYNNPQTDVFVGRADGTGTWVNVSNDP
                     GVTEMDVWLSADGSKVAWAGENDATDEVEVYLANADGSGKTNISNDSTNDDFWPSLSA
                     NGSVVVWNKFYPSTSGWQVFRWVSGVTTNITNSTAVADLAPVVSSSGTKIVWFRFSGA
                     DNEVYLWDNGTATNISNDGLSSDETVTMSADGGLIVWEKYYWATGDYELFQYTTAGGV
                     VNLSHDAAHKDIHPSCSSDGSGVLWLKDLGVSESELYASIAGGPPINVSNDALGKDIH
                     PTTRGRGYR"
BASE COUNT          580 a         1172 c         1114 g          637 t
ORIGIN      
        1 caaggccgcc ggatcggtcg agctgctcaa ggaatccatc gcgctctgcc gtcccggcgg
       61 aaccgtatcc gtggtggcat tctacgagcg gatcgtcgag cagttcgata tcgacggcct
      121 ggtgttcggc gatgtcacgc tccgcgccgt ggcgggcagc ctcgggatgt acaaacccat
      181 cctccgcctg atggcctgcg gtatgctgga cctgacgccc ctcatcaccg ggcggtatcc
      241 gctgttgaac gcccacaagg ccatggcaga catccgagac cgcaacgaca cgcgggtgaa
      301 aatcatgatg gaaggcgagg gatagccgag aggactaccg cgggcccttg ccgcgggcgg
      361 acaggacgct atcgtagccg ctcgggtcgc tgctgacgtt gaaagcgccg ccggagagcg
      421 gggcggcgta cacctccgag tctcccccgg cgcccacacc cagcttctgc cacaccacag
      481 tactgccatc ggccgataga gaagggtgcc gatcggaccg agtcgggtcg gcgctcatgt
      541 tcacggccgc tgcggatccc ttcttggcgt agatgtcgta gtcgcccgac gcggcgcgct
      601 tggcccagac aatcaccgtg ccgtccgcac tcattgacgg gctctcgtcc gacgacgacg
      661 cgtcgttgct gatgttcgtc acggtgccgt tgctccagcg catcacttcg ttgtcgccgc
      721 ccgcgtaccg ttgccaaacc acattcaggc cgtcagcgct gaccaccgcg gacgagtcgg
      781 ctaccgatgg gttgctgctc aggttggtga tggtgcctga agcgtagcgg tagacctgcc
      841 acgtgcgagc agtggggtcg tacttggtcc acacgacaac gcggccatcg ttgctgagcg
      901 acggccagaa gtcgtcacgg gccggatcat tgctgatgtt ctcgaccagg aatcccgcgt
      961 cgtcgctgcg gtagacgtcg ctttcgttcg tgtacgtgtc ctccgccgac caggccacgc
     1021 gcgtcccgtc gccgctcagc cagacgtcga tctcgacatt cacgtcatca ttgctcacat
     1081 tggcccatgt gctcgtgcca tcggcactgc cgatgaacac atccagtccc atgagcgtgt
     1141 cgcggagcat ggccatgcgg gtaccatcgg cgctcagcgc ggggcagtcg taataggtcg
     1201 tcggcacggc cgtgatcgga acggccggca agccatccag ggcactgaca tatatccagg
     1261 tgccctcgcc attgtccttg acccacgccg cccgcggcat cccgctcatg gtcagccatg
     1321 tcacctgaac ggtgaaggtg aacacgtggt ctgcatcgtc ggtgaagacc cacgggagcc
     1381 agtcgctgct ggagccatgg gcgatgctgc catagttcca ctcactatcg tcacaggtgg
     1441 cgccccccgt gatcgccgtg atacgtgcct tcgtggcgaa catggtggcg ccggtgttgt
     1501 tgttgaccct cacccaaccc gtcacccggt tcccactcca ggtgggccgc ccactgcccg
     1561 agtcgccagg gtcggtgctg gtcagcgaca cgcccccctt ctgaggagtg gtgccgcgcg
     1621 tggagacgtc ggccgacccg cccacaacga gctcgaagct gactgtctgg cgcgctgccg
     1681 gctcggtcac caccgcgccg gtcatggtca gaatgcgctg gcggtgcgcc gccaggcgct
     1741 cgcgcgcccc gacatgcccc aatgccgtct cggcgcccgg gctcgccgcg ggaagcggga
     1801 gcaacgggag gttgcctgtc gagcaaccgc tggccaggcc agccaagccg acaccgagaa
     1861 gcacggcgca cggacgccag acgccacgat gagtggacag tggccgcact gagccccgct
     1921 ccttccccca gccactcttc ccctacgcta gcggtagccc cggccgcggg tcgtgggatg
     1981 gatgtccttg cccaaggcat cgttgctgac gttgatcggc ggccccccgg caatggaggc
     2041 atagagctcg gattcgctca cgcccaggtc cttgagccac agcactccac tcccgtcgct
     2101 cgaacagctc gggtggatat ccttgtgtgc cgcgtcgtgg ctgaggttca ccacaccacc
     2161 cgcggtagtg tactggaaga gctcatagtc gccggttgcc cagtagtact tctcccacac
     2221 aataaggccg ccatcggcgc tcatggtgac ggtctcgtcg gacgagagcc cgtcgttgct
     2281 gatgttcgtg gctgtgccgt tatcccacag atatacctcg ttgtcggctc cggagaaccg
     2341 gaaccagacg atctttgtcc cgctggagct gaccaccggg gccaagtcgg ccactgccgt
     2401 gctgttggtg atgttcgtgg tgactccgga tacccatcga aacacctgcc aacctgacgt
     2461 ggacgggtag aacttgttcc agacgacgac gctgccgttg gcgctgaggg acggccagaa
     2521 gtcatcgttc gtcgaatcgt tgctgatgtt cgtcttgcca ctgccgtcgg cgttggcaag
     2581 gtagacctcc acctcgtccg tggcgtcgtt ctcgccggcc cacgcgacct tggagccgtc
     2641 ggcgctgagc cagacatcca tctcggtcac tccagggtcg ttgctcacat tcacccacgt
     2701 ccccgtgccg tcggccctcc cgacgaacac gtcggtctgc gggttgttgt agaccagggc
     2761 catggtcgag ccatcggccg atagtgcggg atagcggtac gtgtgtgtcg ggtcgttggt
     2821 caggggcacc ggtgtgtcgc tgccgctcaa cacgctggcg tagagccact cgccagcgga
     2881 gttctgcttg gaccacaacg ccgtcggggc cccgctgagg gtgagccagg ttacactcac
     2941 gacaaacgag aaggcatgat cggcgtcggt gaagctccat gggagccaac cgctcgacgc
     3001 gccggcttcg atagttccgt agttccatgc actgtcatca taggtcgcgc ccccgctcac
     3061 cgagtcaacc cgggcgcgca cgccatacat ggtggccccg gtcccgttgt tcactcgcac
     3121 ccagcccgta accacatcac cggcccattc gggaatgccg gatccgtcgt tgccagggtc
     3181 agtgctcgtg agggacactc cggccgcatt cggtggtgtt gtgccgcggg atgagacgtc
     3241 gccgaaggtg agctcaaagg tcgcggcgtg cctgccgggc tgtgcggagg acgtctcttc
     3301 ctcgggcggg acggcaatgt ttctgtcgtg ctccctgagc gcgcgcagtt tctcctcaag
     3361 atgcagccgc gcgacctccg cagacggagg gccgacgatc gggatgtcgg gcgcaccggc
     3421 cggggagtcg ctctgcaggc cgcagccgca cgcgaacagc agggcgacgg gcacgaagag
     3481 ggcggcaaac cacgctcgtc gag
//
LOCUS       DAOSPL010000318         3501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26920_length_3501_cov_2.899013, whole genome shotgun sequence.
ACCESSION   DAOSPL010000318 DAOSPL010000000
VERSION     DAOSPL010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26920_length_3501_cov_2.899013"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1703)
                     /locus_tag="PLQ54_07140"
     CDS             complement(<1..1703)
                     /locus_tag="PLQ54_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOS93072.1"
                     /translation="MGDGTERLPVVYTDKAQCRDCYRCVRVCPVKAIRMQGGQASVDP
                     GRCIVCGTCIRECPQGAKRYRSDLARAQALVAGDRPVAVSVAPSFASVYTPWEQRRLA
                     TALRLLGFAHVAETAVGAFYVAQATAEYCRARPGQSHIGTACPAVVSYVERHESELAD
                     RLVPVVSPMVAHGRLIREKLGADTPVVFVGPCVAKKAEADRPENNAVVDCVLTFTELA
                     EWLQAAGVSLAACEESGFDDEPLGHARLFPLEGGCLRTACIKADGLSAEVVAVSGVDE
                     VRDLLSALREGDRPLIVEPLFCAMGCVNGPAVSSALPVFRRRAAVLGYGALPGAPAAP
                     AARELPGLTTTFSPSAPACEPVSEDQIRAALEATGKFSDEDELNCGACGYASCRDKAI
                     AVVHGMAEPEMCIPYMRRLAEQRTDRIIETSPNGIVILDEDLAIVTMNPAFRRLFMCT
                     DAVCGRPISYLMDPAGFERVASGQESVVNTVVSHERYGLVCRHILYALPEDRQYVGIF
                     VNITSGETARRKLGELRARTLMQAQELLEHQIGMAQEIARYLGESAARGEQLVENLMS
                     LA"
     gene            complement(1733..1990)
                     /locus_tag="PLQ54_07145"
     CDS             complement(1733..1990)
                     /locus_tag="PLQ54_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOS93073.1"
                     /translation="MAGPGAEKVKVRVCVGTGCYLRGSQDLLHGLIRRIGELGLSDRV
                     DVQATFCFEQCDRGPSVAVGDQVLERCSLDTVQEALVSLVT"
     gene            complement(2087..>3501)
                     /locus_tag="PLQ54_07150"
     CDS             complement(2087..>3501)
                     /locus_tag="PLQ54_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOS93074.1"
                     /translation="EEIRQIRRLTVELLLANHDESCPTCPKSGTCKLQDLARRLGLEE
                     IRFKPTLRRAAQDRSSPSLVRDPNRCVLCGDCVRVCDEVQGIGAIDFIHRGSHAAVGP
                     AFGKDLNGVECVLCGQCARVCPTGAITPAQEIPAVWRDLEDPSKTVVAQIAPAVRVAL
                     GEAFGLEPGTGLSGQIAAALRVLGFSKVYDTSFAADLTVIEEGAEFVRRATADGPLPQ
                     FTSCCPAWVKFAEQYYPDLLPNLSSCRSPQQMFGSLAKAMLPDLLGVRPEDLVVVSIM
                     PCTAKKFEARREEFATGGVPHVDHVLTTQELARMMEQMGIRLTEMAPESFDMPLGFKT
                     GAGVIFGASGGVTEAVLRYAAEKLGGAAVEEVAFPEVRGDAGLREATVNVGGRRLKLA
                     VAHGLKQARQIAELVRAGRCEYDLVEVMACPGGCVGGAGQPIPGDFDAVRQRAHGLYE
                     TDRGLQFRKSQDNPQLAACY"
BASE COUNT          589 a         1281 c         1098 g          533 t
ORIGIN      
        1 gccaggctca tcaggttctc gaccaactgc tcgccccgcg ccgcactctc gcccaggtag
       61 cgggcgatct cctgggccat gccgatctgg tgctccagca gttcctgggc ctgcatgagc
      121 gtgcgagccc gaagctcgcc gagcttcctt ctcgccgtct cgccactggt gatgttcacg
      181 aagatcccca cgtactgcct gtcctcaggc aacgcgtaca ggatgtggcg gcagaccagg
      241 ccgtaccgct cgtgggagac cacggtgttc accaccgact cctggccgga ggccactcgc
      301 tcgaagccgg cgggatccat gaggtaggag atggggcggc cgcagacggc atcggtgcac
      361 atgaacaggc gacggaatgc cgggttcatg gtcacgatgg ccaggtcctc atcgaggatg
      421 acgatgccgt tcgggctcgt ctcgatgatg cggtcggtcc gctgctcggc cagccggcgc
      481 atgtagggga tgcacatctc cggctcggcc atgccgtgca ccaccgcgat ggccttgtcg
      541 cggcatgagg cgtacccaca cgcgccgcag ttgagctcat cctcgtccga gaacttgccc
      601 gtggcctcca gggcggcacg gatctggtcc tcggatacgg gctcgcaggc cggagccgag
      661 ggagagaacg tggtggtcag tcctgggagc tccctggccg ctggcgcggc cggtgcgccg
      721 ggcagggcac cgtagcccag caccgccgct cggcgccgga ataccgggag ggcggacgag
      781 acggccgggc cgttgacaca ccccatcgcg cagaacagcg gctccacgat cagaggccgg
      841 tcaccctcgc gcagagcgga gagcagatcc cggacctcgt ccaccccgct gacggccacc
      901 acttccgccg agaggccgtc cgccttgata cacgcagtcc gcaggcaacc gccctcgagc
      961 gggaacaacc gcgcatggcc caatggctcg tcatcgaatc cgctttcctc gcaggccgcc
     1021 agggaaacgc ccgcggcctg gagccattcg gcgagctctg tgaaagtcag cacacagtca
     1081 actacagcgt tgttctccgg tcggtccgcc tcagccttct tcgcgacaca cggacccaca
     1141 aacaccaccg gtgtgtcggc gccgagcttc tcgcggatca gccgcccatg ggcgaccata
     1201 ggcgacacca ctggcaccag gcggtccgca agctcggact catgccgctc cacataggag
     1261 accacggcag gacaggcggt cccgatgtgg gactgcccgg ggcgcgcccg gcagtactcg
     1321 gccgtcgcct gggcgacgta gaaagctccc acggccgtct ccgccacgtg cgcgaagccc
     1381 agcagccgga gcgccgtcgc cagacggcgc tgctcccaag gcgtgtagac cgaggcaaac
     1441 gacggcgcca cgctcacggc caccgggcgg tcccccgcca ccagtgcctg agctcgggcc
     1501 aggtcactcc gataccgctt cgcgccctgg gggcactcac ggatgcacgt gccgcacaca
     1561 atgcagcggc cgggatcgac cgatgcctgc ccgccctgca tccgtatcgc cttgacgggg
     1621 cagacccgca cacatcggta gcaatcccgg cactgcgcct tgtcggtgta gaccacgggg
     1681 agccgttcgg taccgtctcc cactgcgcga gcacctcaca ttgtgagaga tctcacgtga
     1741 cgagactcac aagcgcttcc tgcaccgtat cgagcgaaca cctctccaat acctggtccc
     1801 ccacggccac ggatggcccg cgatcacact gctcgaagca gaaggtggcc tgcacgtcca
     1861 cccggtcgct caaacccagc tcgccgatcc tgcggatcag cccgtgcagc aggtcctgtg
     1921 acccccgaag gtagcacccg gttcccacac acacgcggac cttcaccttc tcggccccgg
     1981 ggcccgccat cagcgtgatg tcctcgtccg cgattcgctt ccgactctgg tagtgggtgt
     2041 gcaggaggca gtgggctttc tcgccgccta cttctcccag tgtcgtctag tagcacgcgg
     2101 cgagctgcgg gttgtcctgg gacttccgga attgcagtcc tcggtccgtc tcgtaaagcc
     2161 cgtgggctcg ctgccgaacc gcatcgaagt ccccggggat gggctgtccc gcgccgccaa
     2221 cgcacccgcc cgggcaggcc atgacctcca ccaggtcata ctcacaccgg cccgcgcgca
     2281 ccaactcggc gatctggcgc gcctgtttca gcccgtgcgc gacggctaac ttcagccggc
     2341 gcccgccgac gttgacggtc gcctcgcgca ggcccgcatc accgcggacc tccgggaaag
     2401 cgacctcctc gactgcggcg ccccccaact tctccgccgc atagcgcagc acggcctcgg
     2461 ttactccgcc cgacgcgccg aagatcacgc ctgcacccgt cttgaagccc aggggcatgt
     2521 cgaaggattc cggggccatc tccgtgagcc ggatacccat ctgctccatc atccgcgcca
     2581 gctcctgggt ggtcagcacg tggtcgacgt gcggtacgcc gcccgtggcg aactcctccc
     2641 gcctggcctc gaacttcttc gccgtacacg gcatgatcga caccacaacc aggtcctcgg
     2701 gccgtacgcc gagcagatcg gggagcatgg ccttggcgag cgagccgaac atctgctgcg
     2761 gcgaccggca cgacgacagg ttggggagca aatcggggta gtactgctcg gcgaacttca
     2821 cccaggctgg acagcatgac gtgaattgcg gcagtgggcc gtcggcagtc gcccgccgca
     2881 cgaactccgc gccctcctca attaccgtca ggtcggccgc gaacgacgtg tcgtagacct
     2941 tcgagaagcc cagcacgcga agcgccgcgg cgatctgacc cgacagtccc gtgcccggtt
     3001 cgagcccaaa cgcctcgccc agggccaccc gcaccgctgg cgcgatctgg gccaccaccg
     3061 tcttggatgg atcctccagg tcacgccaca cggcggggat ctcctgcgct ggcgtgatgg
     3121 caccggtggg gcagacccga gcacactggc cgcagagcac gcactcgacg ccgttcagat
     3181 ccttgccgaa ggccgggccc acggcagcgt gactcccacg gtggatgaag tcgatggccc
     3241 cgatgccctg gacctcgtcg cacacccgca cgcagtcacc gcacagcacg caccggttcg
     3301 ggtcgcgaac cagcgacggc gacgaacggt cctgagcggc tctgcggagc gtgggcttga
     3361 accggatctc ctcgagcccc agccggcggg ccaggtcctg aagcttgcag gtgccactct
     3421 tggggcacgt ggggcagctc tcgtcgtggt tggccagcaa gagctcgacc gtcagccggc
     3481 ggatctggcg gatctcctcg g
//
LOCUS       DAOSPL010000319         3496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_26973_length_3496_cov_3.288288, whole genome shotgun sequence.
ACCESSION   DAOSPL010000319 DAOSPL010000000
VERSION     DAOSPL010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26973_length_3496_cov_3.288288"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="PLQ54_07155"
     CDS             complement(<1..1485)
                     /locus_tag="PLQ54_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS93075.1"
                     /translation="MRGRSNFRRFLGYLKPYRWRASELLFWTFVSALLGLPMPVLLGI
                     AVDRVPGLVKDGNAREAWGVVVWTVGARIALGVGASLLSLRHRALTLSIGQRLIFDLR
                     QQLYRHLQRLSMRYYETMQTGQIMSRVLWDVEAVQAISTGQLAQLIVDFVRLVVIATY
                     LFIRDWRIGLLTCIVLPLYVINYYFFRNKIHEVAAETREKFSQITGSLQEKVAGARIV
                     KSFVRERSESRQFVHETRENLFLNIRLGLWSGGLNTTASFISDLGTQLAVLYCWYRVV
                     WGQHMTAGDVVVISSFVAQMYGPVLNITTINDAIVRASTALDRIFGVLDTTPDVAEAP
                     DALPVENVQGRIEFRDVYFSYQPDELVLKNVNFVAEPGTVNALVGPSGGGKSTIINLI
                     PRFYDPIAGEILLDGIPLTKLKLNALRQQIGIVLQETFLFTGTIKENIKYGKPDARDD
                     QVEAAAKAANAHEFIMEQRDGYETEIGERGLRLSGGQKQRIAIAR"
     gene            1735..3081
                     /locus_tag="PLQ54_07160"
     CDS             1735..3081
                     /locus_tag="PLQ54_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOS93076.1"
                     /translation="MPVAEGGGSDMVTRNSRMALAVVLLAAWAAYPAVAAEPPQWIQG
                     DLTRAKSEAARANRPILLLLYAPGSRACESAQERVFSQPEFARAAEGWILAKLDVSRD
                     KGLVEKQELEALPTTAFITAEGEELGRIVGVSSIKSYLSAMADGLLIFRLKPIDPPKL
                     STADADEYVAALLRLERGGIAAAVLKSRVESGQLSEAELRRSQILLGSLQCLLGDVEA
                     GRQTLAEVLAGARVVEPVERVRQAVDHLTALGLMEPGAVVPAEIDKPAGEPIGAIVAR
                     KVTPEELVQTVEQMGALARGETPAGMTEADLGAFFLRLGNALEAMACLDRALASASDG
                     EAEETLITLRLAAACIIGPDPGRGVRLIDELLTKYPESGQRPFAMYLAAIGCWRVGDE
                     TKYRIVVDYLHEHFPDTVWARRAEGLTATLKALKQQYEAPAGTEGAPEQPEATPKP"
     gene            3188..>3496
                     /locus_tag="PLQ54_07165"
     CDS             3188..>3496
                     /locus_tag="PLQ54_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS93077.1"
                     /translation="MIELGIGVIGYGFIGKVHTYAYRNLHFYYDPVPARVRLVGVCTS
                     RAETAERARAHGGFDVGVTRIEELLSRDDVHAVHICTPNALHHDAALAAIAAGKHVYC
                     D"
BASE COUNT          638 a         1117 c         1141 g          600 t
ORIGIN      
        1 gcgggcaata gcaatgcgct gcttctgccc ccccgagagc cgcaggccgc gctcgcctat
       61 ctcggtctca tagccatcgc gctgttccat gatgaactcg tgggcgttag cggccttcgc
      121 cgccgcctca acctggtcgt cccgggcgtc tggcttgccg tatttaatat tctccttgat
      181 cgttcctgtg aacaagaacg tctcctgaag cacaatgccg atctgctggc gaagcgcgtt
      241 tagtttgagc ttggttaagg gaatgccgtc cagcagtatc tcgccggcaa tcgggtcgta
      301 gaagcgcggg atcaggttga tgatcgtgct cttgccaccg ccgctaggcc caaccagggc
      361 gttgaccgtg cccggctccg cgacgaagtt gacgttcttg agaaccagtt catcgggttg
      421 atatgagaag tagacatcgc ggaactcaat ccggccctga acgttctcga caggaagcgc
      481 atcgggagcc tccgccacat ccggggtggt atcgagcacg ccgaagatgc ggtcgagggc
      541 ggtggaagcc cgcacgatgg cgtcgttgat cgtggtgatg ttgagcacgg gcccatacat
      601 ctgcgccaca aacgaggaga tcacgaccac gtcacctgcg gtcatatgct ggccccacac
      661 cacacggtac cagcagtaga gcacggcgag ctgtgttccc agatcggaga tgaacgacgc
      721 ggtggtgttg aggccgccac tccacagccc caggcggatg ttcaggaaga ggttctcgcg
      781 agtctcgtgg acgaactggc ggctctcact ccgctcgcga acgaacgatt tgacgatacg
      841 agcaccggcc actttctcct ggaggctgcc ggtgatctgc gagaacttct cgcgcgtctc
      901 ggccgcaacc tcgtggatct tgttgcgaaa aaagtagtag ttgatgacat acagaggaag
      961 cacgatgcac gtcagcagac cgatgcgcca gtctcggatg aacagatagg tcgcgatgac
     1021 caccaggcga acgaagtcga cgatgagctg ggcgagttga cccgtggaga tagcctggac
     1081 tgcctcgaca tcccacagaa ctcggctcat aatctgcccg gtctgcatcg tctcgtagta
     1141 ccgcatcgag agccgctgca gatggcgata gagctgctgc cgaagatcga agatgaggcg
     1201 ctgcccgatg ctgagcgtca gggcccggtg ccgaagtgac agcagtgagg ccccgacccc
     1261 gagggcaatc cgggcgccga ccgtccagac gaccaccccc cacgcctccc gagcgttgcc
     1321 atccttaacg agccccggta cccggtcgac cgcgatgcca agcagcacgg gcatgggaag
     1381 accaagcagg gcgctaacga aggtccagaa cagcagctcg gacgcgcgcc atcggtaggg
     1441 tttgaggtac ccgagaaacc tacggaagtt gctgcgtccc cgcacgggtc accccccgct
     1501 ccgcccctcg agagccatca tcgtgggagc gcgtcccaac gcccatgagc tgtcggacgc
     1561 accccgcaac tataccctct ctgccttcca cgagccaccc cgcaagcgcc gtgcggtgct
     1621 tgcgggagtc gtgcatgcgc cggcatggga ttcggtggcc gtgtgcgaac atacctccga
     1681 caggggcaag ggaactcgcg aagccgccgg cgcgtcagcg aagaggtcat gtccatgccg
     1741 gtagccgaag gcggtgggtc tgacatggtt acgcggaact cccgtatggc gttggccgtc
     1801 gttctcctgg ccgcgtgggc ggcgtacccg gcggtggctg ccgaaccgcc ccaatggatc
     1861 cagggcgacc ttacgcgggc caagtccgag gcggctcgtg ccaaccggcc catcctgctg
     1921 ctgctgtatg cgccggggtc acgggcgtgt gagtccgccc aggaacgtgt gttcagccag
     1981 ccggagttcg cccgggccgc cgagggctgg atcctggcca agctcgacgt ctcccgcgac
     2041 aaggggctcg tcgagaagca ggagcttgag gccctgccga ccacggcttt catcaccgcc
     2101 gaaggtgagg aattgggccg catcgtcggt gtcagctcca tcaagagcta tttgtctgca
     2161 atggccgacg ggttgctgat cttccggctc aagcccatcg acccgccgaa gctcagcact
     2221 gccgacgccg acgagtacgt agcggccctc ctgcggctcg agagaggtgg cattgcggcc
     2281 gctgtgctca agtcacgggt ggaatcggga cagctgagcg aagccgagct tcggcgatcg
     2341 cagatcctgc tcgggtcatt gcagtgcctc ctgggcgacg tcgaggccgg gcgccagacg
     2401 ttggccgagg tattggcggg agcgcgcgtt gttgagcccg tcgagcgggt gcgccaggcg
     2461 gtggatcatc tgacggcctt ggggctgatg gagcccggtg cggttgtgcc cgctgagatc
     2521 gacaaacccg ccggggagcc catcggtgcc atcgtcgctc ggaaagtcac accggaggag
     2581 cttgtgcaga ccgtcgagca gatgggcgct ctggcgcgag gcgaaacccc tgctggaatg
     2641 accgaagccg atctgggggc gttctttctg cggctgggca atgcgctcga ggccatggcg
     2701 tgtctcgacc gcgcgctggc ctcggcttcc gacggcgaag ccgaggagac actcatcacc
     2761 ctccggttgg cggccgcatg cattatcggg cccgacccag gccgaggcgt tcggctaatc
     2821 gacgagctgc tgaccaagta cccggagtcg ggtcagcgcc cattcgccat gtaccttgca
     2881 gccatcgggt gctggcgtgt gggcgatgag accaagtacc ggattgtcgt ggactacctc
     2941 catgaacact tcccggacac cgtctgggcc cgccgtgccg aaggcttgac ggccaccctc
     3001 aaggcactca agcagcaata cgaggccccc gcgggcaccg agggagcccc cgagcagccg
     3061 gaagcaaccc ccaagcccta acgagcgagc gtcgggccgg cctccccgtt gacgctccgg
     3121 gggggcgtgc ctagaatgcg ttcgaggtcg ccaccccacg catcggttcg cctcgatcgg
     3181 agcgcccatg atagagctcg gtatcggcgt gatcggctac ggcttcatcg gcaaggttca
     3241 tacctatgcc taccgcaacc tgcatttcta ctacgatccg gtgccggccc gggtccggct
     3301 ggttggcgtc tgcaccagcc gggcggagac cgcggaacgt gcccgggccc acgggggctt
     3361 cgacgtgggt gtcacgcgga tcgaggaact cttgtcgcgg gacgacgtgc acgcggtgca
     3421 catctgcacg ccgaacgcgc tccatcacga tgcggccctg gccgcgatcg cggccgggaa
     3481 gcatgtctac tgcgac
//
LOCUS       DAOSPL010000320         3489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_27031_length_3489_cov_4.013978, whole genome shotgun sequence.
ACCESSION   DAOSPL010000320 DAOSPL010000000
VERSION     DAOSPL010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27031_length_3489_cov_4.013978"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PLQ54_07170"
     CDS             <1..914
                     /locus_tag="PLQ54_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93078.1"
                     /translation="HNDAMDALAETGVPGLILAIALVGTCLMPAIRRSALEGRGLAVA
                     VSAALIGWCANGLLNSSLANPATEPACLLILGLAVSASSERRPWAVPRPLAWFAPFLV
                     LLPLSLAGQRVIRTVWAWSVVSQIGLASEQRAILREGGEPPLSRGSAGPGAAAALRRA
                     IRIDPAYNALWQLLAAVTAASEPGVARAALLRSIELVPSYAGSHHALGLLLAAEGHAV
                     RAREELRTAVRLDPYNPDYRIDLAEQLGPDQRFAALRNLARAAQLLLMTQEVVTARFG
                     PASREAEAVNEGFERVNRVRRQVMGGR"
     gene            981..1916
                     /locus_tag="PLQ54_07175"
     CDS             981..1916
                     /locus_tag="PLQ54_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS93079.1"
                     /translation="MTESFENLVLTGIIHPMAFPAAAKGDGPVLDTLATIAHDDFFTA
                     VEVRRAASPDILEGERKLLSEAMMEVIVAGQPPLLGAKLNLNALDDSARQAAVADVRL
                     SIDEAYALGAPIVAVLSGPDPGDADREAGMAALTQSLVELADYSTSRAAGKKPVFISL
                     ETFDRAIDKRCLIGPTALAAELMDRVKQSVANVGLTVDLSHLPLLGETADEMLETAAP
                     HLIHVHIGNAYIDQTDDPAYGDQHPRFGYPGSLNGVTAVTRFLRVLKRIGYFEAKVPT
                     RKPVISFEIKPVPGESSEAIIAGAKRVFKEAWYAA"
     gene            1968..2807
                     /locus_tag="PLQ54_07180"
     CDS             1968..2807
                     /locus_tag="PLQ54_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOS93080.1"
                     /translation="MQIGILTAPFGNEPVANAIQFAAKAGAEALEITTGRGSGHFDAV
                     TATDADIAALGKLVADNGLTISSFAHYRQVFSEDPADEAAAAADLKAVIDTAAKLKVG
                     VVCVLAGMPYGGKSKMEIIETRAPEFWPPVLAYARERGIKLAMENWFATLLQGLDTFQ
                     RIFEVVPDENFGLNFDPSHLYWQGIDYLAAVEQFAPRIFHTHAKDTEIREHKLRYVGS
                     LASGWWRYVIPGYGGIDWGQYIARLRSNGYNGVLSIEHEDSAVGREEGFIQGIKYLRQ
                     FCS"
     gene            complement(2993..>3489)
                     /locus_tag="PLQ54_07185"
     CDS             complement(2993..>3489)
                     /locus_tag="PLQ54_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93081.1"
                     /translation="SNIIKGLRERPPDAPVSYVVTQWPAGQEPPTQLSEQGRFECIQA
                     LGELRPLTPRVIDALLLNIGFVPETWPYRVSPAAVAAAKTGLPVRWRIQCMGLHASRE
                     KRNKLLDALGVMLGRFAAEPMKWEARERGSYPELEADIEYLETTYAGTKALHEVIPEA
                     PPEG"
BASE COUNT          547 a         1286 c         1084 g          572 t
ORIGIN      
        1 cccacaacga cgcgatggat gccctggccg agacgggcgt ccccgggctc attcttgcca
       61 tcgccctggt cggcacatgc ctcatgccgg cgatccgccg gagcgccctc gaggggcgcg
      121 ggctggccgt cgcggtctcg gccgccctca ttggctggtg cgccaacggc ctcctcaaca
      181 gctcgctcgc caacccggcc actgagccgg cgtgcctgct aatacttggc ctcgccgtat
      241 ccgcttcatc ggagcgtcga ccgtgggccg tgccccgccc attggcctgg ttcgccccgt
      301 ttctggtgct gctgccgctg tccttggccg gccagcgagt catccgcacc gtgtgggcat
      361 ggtccgtcgt gtctcagatc gggctggcat ccgagcagag ggccatactc agagaaggcg
      421 gcgagccccc gttgtcccgc ggctccgctg gacccggtgc cgccgcggcg ctccgccgag
      481 ccatccgcat cgacccagcc tacaatgctc tgtggcagct cctcgcggcg gtcaccgcgg
      541 cctccgaacc cggcgtggcc cgggccgcgc tcctgcgctc tatcgagctc gtgccctcct
      601 acgccggctc acaccatgcc ctcggcttgc tgctggccgc cgagggccac gccgtgcgcg
      661 cacgggagga gctccgcacc gccgtgaggc tcgatccata caacccggac taccgcatcg
      721 acctggctga gcaattgggg cccgaccagc ggtttgcggc actccggaac ctggccagag
      781 ccgcccagct cctactgatg acgcaggagg tcgttacggc ccggttcggc ccggccagcc
      841 gcgaggccga ggcagtaaac gagggcttcg agcgcgtcaa ccgcgtgcgc aggcaggtca
      901 tggggggccg ctagaccctg tgctataatg ccgcccgttc gtcttaccgc ctccgtccgc
      961 actcacggga gggaccccga ttgactgaga gtttcgagaa tctcgttctc actggcatca
     1021 tccatcctat ggcattccca gccgcagcga agggtgacgg tcccgtcctc gataccctcg
     1081 cgaccatcgc ccacgatgac ttcttcaccg ccgtcgaagt ccgccgggcg gcgtcacccg
     1141 acatcctgga gggcgagagg aagctgctct ccgaggccat gatggaggtc atcgttgccg
     1201 gccagccccc gctcctgggc gccaagctca acctcaacgc tctcgacgac tccgcccgtc
     1261 aggccgccgt ggctgatgtg cggctctcca tcgacgaggc gtacgcgctg ggtgcgccca
     1321 tcgtcgctgt gctttccggg cccgatcccg gcgatgccga ccgcgaggcc ggcatggcgg
     1381 ccctcacgca gtcgctggtc gaactcgccg actactccac gtctcgcgcc gcggggaaga
     1441 aaccggtctt catctcgctc gagaccttcg accgcgccat cgacaagcgc tgcctcatcg
     1501 gcccaaccgc actggcggcc gagctgatgg atcgcgtgaa gcagtctgtc gccaacgtcg
     1561 gtctgaccgt cgacctcagt catctgccgc tcctcggcga gaccgccgac gagatgctcg
     1621 agaccgccgc gccgcacctg atccacgtcc acatcggcaa cgcctacatc gaccagaccg
     1681 acgacccggc ctacggcgac cagcacccgc gcttcggcta tccgggtagc ctcaacggtg
     1741 tcacggcagt cacgcggttc ctgcgggtgc tcaagcgcat cggctacttc gaggccaagg
     1801 tgcccacgcg caagcccgtc atcagcttcg agatcaagcc cgtacccggt gagagctccg
     1861 aggcgatcat cgcgggcgcg aagagagtgt tcaaggaagc ctggtacgcc gcttagccgc
     1921 gtccctgcgg cttgcgatat cggaagagag acagggggac accgttcgtg cagatcggta
     1981 ttctgaccgc tccattcggc aatgagcccg tcgccaacgc catccagttc gccgccaagg
     2041 ctggggccga ggcgctggag atcaccaccg gccgcggcag tggccacttc gacgccgtga
     2101 cggcgaccga tgcggacatc gccgctctcg gcaagctcgt ggcggacaac ggcctgacca
     2161 tcagcagctt cgcccactac cgtcaagtct tcagcgagga ccccgccgat gaggcggccg
     2221 ccgccgcgga cctcaaggcc gtcatcgaca cggcggccaa gctcaaggtc ggcgtcgtct
     2281 gcgtgctcgc tggcatgcct tacggcggca agtcgaagat ggagatcatc gagacccgcg
     2341 cgcccgagtt ctggccgccg gtcctcgcct acgctcgcga gcgcggcatc aagctcgcca
     2401 tggagaactg gttcgccacg ctgcttcagg gcctcgacac cttccagcgc atcttcgagg
     2461 tcgtccccga cgagaacttc ggcctcaact tcgacccgtc acacctctac tggcagggaa
     2521 tcgactacct ggcggccgtc gagcagttcg ccccgcgcat cttccacacc catgccaagg
     2581 acacggagat cagagagcac aagctgcgct acgtcggcag cctggccagc ggctggtggc
     2641 gctatgtgat cccgggctat ggcgggatcg actgggggca gtacatcgct cgcctgcgaa
     2701 gcaacggcta caacggcgtg ctgagcatcg agcatgagga ctcggccgtc ggccgcgaag
     2761 agggcttcat ccagggcatc aagtaccttc gccagttctg ctcgtaggta gctggatcgg
     2821 caccggggcg ccgtcggctc agcctggcgg cgccctgcgg attccacccc cgccccgcac
     2881 cccgctgaca tcgagccgcc gcgtccacgc ggcgtgacgc ccttcgacag ccacaggagg
     2941 cgccgcgaat gacgatcccc tgatctctga cccctgaccc ctgcctttcc cgctaccctt
     3001 ccggcggcgc ctcggggatc acctcatgca gggctttggt gcctgcatag gtcgtctcga
     3061 gatactcgat gtccgcctca agctcggggt acgagccccg ctcccgcgcc tcccacttca
     3121 tcggttcggc ggcgaaacgc cccagcatca cgcccagggc gtccagcagc ttgttgcgct
     3181 tctctcggct ggcgtggagc cccatgcact ggatacgcca ccgcaccggc aagcccgtct
     3241 tggccgcggc aacggcggcg ggcgatacgc gatacggcca cgtctctggc acgaacccga
     3301 tgttcagcag cagtgcgtcg atgactcgcg gcgtcagcgg ccggagctcg cccagcgcct
     3361 ggatgcactc gaaccgtccc tgctccgaga gctgggtcgg cggctcctgc ccggccggcc
     3421 actgggtcac gacataggac accggcgcat cgggtgggcg ctcgcgcagg cccttgatga
     3481 tgttgctgt
//
LOCUS       DAOSPL010000321         3488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_27046_length_3488_cov_3.119720, whole genome shotgun sequence.
ACCESSION   DAOSPL010000321 DAOSPL010000000
VERSION     DAOSPL010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27046_length_3488_cov_3.119720"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PLQ54_07190"
     CDS             <1..562
                     /locus_tag="PLQ54_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93082.1"
                     /translation="GTIQAVFDGRALLHQIRADIDGTPVTMPTPSGYALFQPEHWEIT
                     WNIGGEWDVVDGGELVHYSLDARGGADRDSDSNALIQAFQLDLDAGTVQRRASINVDG
                     TLTRTDSEGTTTSHWSASYGTAGLLPAETLPANWDIPARSFSRVDDGTTVTIHWTHIA
                     PDNPPPTGAAAYPSSIARTRMLRSPG"
     gene            780..1973
                     /locus_tag="PLQ54_07195"
     CDS             780..1973
                     /locus_tag="PLQ54_07195"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HOS93083.1"
                     /translation="MRAIAWAIGALALSAGSSWGQPPTPAAFDHPCMYLNADEIARLR
                     QRIASDAPARTMADRLVAGADQLLARTPQPVTGPFRNVLIPPLPSDPSQVTLVTTHGQ
                     LARRDLAAARDLGIAYALTGDERYAAKVAEFASAWASAMEAKLPNTYSAWLVVAQTLP
                     ALYYAVDLCWDAAPMTDELKGQIKAWAGAIAAQAKEKHPLCTPYLTAFNLNLIASSGI
                     ICEDRGLVEFAYNAPDNPDTFQGLLRGSKDAATGKKMPGLFDSNGKANYEANQDAPVS
                     RAIQVIKALTYVAELARHQGQDLYAFEYKGAGLRSAYLYYAPDFDGTRKAPDLSPFRG
                     AKGDAAVYELAFARYGEPAMLSVLRALGRDLVDPHVLGPIGLTHGVPTDLAGAVRPPS
                     TAGRR"
     gene            2142..2462
                     /locus_tag="PLQ54_07200"
     CDS             2142..2462
                     /locus_tag="PLQ54_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93084.1"
                     /translation="METFGKAIGIAVFVAGMALLGYVFYLAYMFPVLFHHALGSGTPQ
                     WLPIPSARVTTPSALEGMSPLVQFMAEAGVYLLTLLVLGGLAALIAHKGASLIGVLGG
                     KKGE"
     gene            2469..>3488
                     /locus_tag="PLQ54_07205"
     CDS             2469..>3488
                     /locus_tag="PLQ54_07205"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOS93085.1"
                     /translation="MAPRQHSRGLCMAMAGLLISWAGSAWDAWAADAGAGTAALARQF
                     LDQGARALAEGADEVALQAFAQATQVEPDCQIARLGSGVAQLRLGRRAEADRSFRLAG
                     GRLPAAATVGQALTSLAAGEVLAARSLLQQSIDLGVQPVAAVRATLAYCALLAGDCAD
                     ARAEARLVLDSDPANYLALMVMAEAGTRLGTAPEAGNTLRQILDFRARSLITPARQLA
                     LLGPEALVSGRSPIEGMGAVSAAARFERPGIRIITPLTGTTVRGKVPLLASVIGEARP
                     LYVTFYVNGGRIHATDKPPYRYTWDVYDLVPGEYLISVRAQESYLGAELYHDQVVVHV
                     TRESGL"
BASE COUNT          533 a         1261 c         1142 g          552 t
ORIGIN      
        1 tggaaccatc caggccgtct tcgacggacg cgccctcctc caccagattc gggccgacat
       61 cgacggtacg cccgtaacca tgcccacccc gtccggctat gccctgttcc aacccgagca
      121 ctgggagatc acctggaaca tcggcggcga gtgggatgtg gtcgacggcg gggagctcgt
      181 ccactactcg ctggacgccc gcggcggcgc agatcgtgat agcgactcga atgccctgat
      241 ccaggcattc cagctcgatc tcgacgccgg taccgtgcag cgccgcgcct ccatcaacgt
      301 cgacggcacc ctgactcgta ccgactcgga ggggaccacg acctcacact ggtcggcgag
      361 ttacggcacc gccggcctgc tgccggcgga gacgcttccc gcgaactggg acatccccgc
      421 ccgcagcttc tcacgcgtcg acgacggcac gaccgtcacc atccattgga cccacatcgc
      481 gccggacaat ccacccccga cgggcgctgc cgcctatcca tcatcgatcg cccgcacgcg
      541 tatgctcaga agccccggct agcgcggcag ggccgcatga ggcgtcgcga cggccgcact
      601 cgcggccgtg tcactccgcc ggcgctttga cagccccact gggccgcgca tatagtgccg
      661 accggcgtct gcaccgggcg cggggcacct cgccgggctc cagaacgtcc tactgctggt
      721 gggcgcggcc cgctgtggcg gcctggccgc aagtggcacg gcatcggagg cgtgtcgggt
      781 tgagggctat cgcttgggcg atcggtgcac tggcgctatc cgccggctcc tcgtgggggc
      841 agccgccgac gccggcggcg ttcgaccatc catgcatgta cctcaacgcg gacgagatcg
      901 cgcgtctgcg gcagcggatc gccagcgacg cgccagcgcg gaccatggcc gaccgactcg
      961 tcgccggtgc cgaccagctc ctcgcgcgca ccccacagcc agtcaccggg ccattcagga
     1021 atgtgctcat tccgccgctg ccgtccgatc catcccaggt gaccttggtc accacccacg
     1081 ggcagctggc gcgccgcgat ctcgctgccg ctcgtgacct cggcatcgcc tacgccctga
     1141 cgggcgacga gagatatgcg gccaaagtgg ccgagttcgc gtccgcctgg gcgagcgcca
     1201 tggaagcgaa gctgccgaac acgtactcgg cctggctcgt ggtggcgcaa accctcccgg
     1261 ccctctacta cgccgtggac ctgtgctggg acgccgcccc tatgaccgac gagctcaagg
     1321 ggcagatcaa ggcctgggcc ggcgccattg cggctcaggc caaagagaag cacccactct
     1381 gcacgcccta tttgaccgcg ttcaacctca atctgattgc gtcctcgggg atcatctgcg
     1441 aggaccgtgg gctggtggag tttgcctaca acgccccgga caatccggac acgttccagg
     1501 ggctgctccg cgggtccaaa gatgcggcga ctgggaagaa gatgcccggg ctgttcgaca
     1561 gcaatggcaa ggccaactac gaggcaaacc aggacgcgcc ggtgtcacgc gcgatccagg
     1621 tgatcaaggc cctcacctat gtggccgagc tggcgcgcca tcagggacag gacctgtatg
     1681 cgtttgagta caagggcgcc gggttgcgca gcgcgtacct ctactacgcg cccgacttcg
     1741 atggcactcg caaggcgccc gatctgtccc cgttccgagg cgccaagggc gacgccgcgg
     1801 tctacgaact ggccttcgcc cgctacggtg agccggccat gctctccgtg ctccgggcgc
     1861 tgggccgcga cctggtggac ccgcacgtcc tcgggcccat cggtctcacg catggtgtgc
     1921 cgacggatct ggctggcgcg gtccggcccc cctccactgc cggaagacgg tgactcgggg
     1981 gcaggacatc ccatcccgct gcccaaacaa cacggagagt gtgcgctgcc gcagcgattg
     2041 agccgcgccg ggcgcgtcgg atatactgcc agcgccttca ccgagtggcg tcgggctgaa
     2101 gcggccattc ggagtttgga cgagcgggga ccgaggcggg cttggagacc ttcggcaagg
     2161 ccatcggaat agcagtattc gtggcaggca tggccctgct cgggtacgtg ttctacctgg
     2221 cctacatgtt ccccgtgctg tttcatcatg ccctgggctc cgggactccc cagtggctgc
     2281 cgatcccctc ggcccgggtc accacgccga gtgcgctgga ggggatgagc ccgctcgtcc
     2341 agttcatggc cgaggcgggc gtctatctcc tgacgctcct cgttctaggt ggacttgccg
     2401 cacttatcgc ccataagggc gcctcgctga tcggtgtcct gggcggcaaa aagggagaat
     2461 gaccctcggt ggcacctcga cagcattcgc gggggctctg catggccatg gcaggcctcc
     2521 tgatctcatg ggcgggatcc gcctgggacg cctgggccgc ggatgcgggg gccggtacgg
     2581 ccgccctggc ccgtcagttc ctcgaccaag gcgctcgcgc gctggccgag ggtgccgacg
     2641 aggtggccct ccaagccttc gcccaggcca cccaggtcga gccggactgc cagatcgcgc
     2701 gccttggcag cggcgtggcg cagctgcgcc tggggcgccg ggccgaggcg gatcggagct
     2761 tccgcctggc aggcggccgc ctgccggccg cggccacggt gggccaagcc ctcacctccc
     2821 tggccgcggg cgaggtgctg gccgcgcgtt cgctgctcca gcagtccatc gacctcggtg
     2881 tgcagcccgt cgccgccgtg cgcgccacgc tggcctactg cgccctgctg gccggcgact
     2941 gcgccgacgc gcgcgcagag gcccggctcg tcctcgacag cgatcccgcc aactacctgg
     3001 ctctcatggt aatggccgag gctggcaccc gtctcggcac cgcccccgaa gcggggaaca
     3061 cgctgcggca aatcctggat ttccgcgccc gaagcctgat cacgccggcc cggcaactcg
     3121 cgcttctggg acccgaagct ctggtgtccg gccgcagtcc gatcgagggt atgggagccg
     3181 tctcggccgc cgcccggttc gaacgccccg ggatccgaat catcaccccg ctgaccggta
     3241 ccacggtgcg cgggaaagtc cccctcctgg cctcggtcat tggcgaggcg cggccactgt
     3301 atgtcacctt ctacgtgaat ggcggccgca tccacgccac cgacaagcct ccgtaccggt
     3361 acacctggga tgtctacgac ctcgtccccg gcgagtacct gatctccgtc cgcgcccagg
     3421 agagctatct gggggccgag ctctatcacg accaggtcgt ggtccatgtg acccgtgaga
     3481 gcggtctg
//
LOCUS       DAOSPL010000322         3487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_27058_length_3487_cov_3.465909, whole genome shotgun sequence.
ACCESSION   DAOSPL010000322 DAOSPL010000000
VERSION     DAOSPL010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27058_length_3487_cov_3.465909"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PLQ54_07210"
     CDS             complement(<1..545)
                     /locus_tag="PLQ54_07210"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOS93086.1"
                     /translation="MPSFIRFQKSAGSRRASKIILAIIAAIFGVGAFFSFGGSPGRNP
                     MGSIPDAFRVNGKTINMAEFHQALENYENQMQMYGGSKGDPTRERAMQLAVMKELIVD
                     TALQQDAARRKLSASSEDIEKARLEWIQQQTASLGEGAAKQKALIEENMTEAEFNERI
                     QQRARSLGMQQYFTHKIVMEKL"
     gene            935..1615
                     /locus_tag="PLQ54_07215"
     CDS             935..1615
                     /locus_tag="PLQ54_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93087.1"
                     /translation="MNARNRFLVSTVFVSLVCVLGAAEAFDLLFVPLADTMPKGKAEL
                     QSAYIDKPLGAPAGGVVLNNLYYGITDRLEISAHSAVFMGVPGNPPPMFQVNAEYRLL
                     DSKKPNHPTVSVGAYNLNGGNFLNYNAVQPYIVVSHNVNIPDKGKPEWDDPLVRLTAG
                     VGTAGHGLFGSALSVFDPHFAAEAGWHGGRYHVCVNWMLGDKSQYHVTPAIWNGSPAL
                     KVAYQHKF"
     gene            1737..2843
                     /locus_tag="PLQ54_07220"
     CDS             1737..2843
                     /locus_tag="PLQ54_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS93088.1"
                     /translation="MTHRERLLTALDLGVPDQVPVTWELVGRCAYAHTGDAGWRGQCD
                     THRMVGSSIFNLQGVGPEIECELPAGFSVEREELGVEDGWTLTETRIVTPSGTLRECR
                     KTGGIPGDPLLGKCTRYFVQNREDYEILATYLEARARCARPRTHESSVALDYVRDDGL
                     VNHWMGDSLYGLANYRDSEEFLVDLVEIPGVLKELLRHLHVEREIGLEAFNSSAADVL
                     VWDICWSSTSLLNPQMVHEFVLPEARWVVENLAPGKRVVFFTTGRIRGVLDDLARCRP
                     HGIQHLDVLGDCDLAEVKQTFGRRFCLMGNYSPVVLAHGTPEEAQREAKRCLDAAMDG
                     GGYIITTSDEVPADARLENMRAVVEYVGREGRYA"
     gene            2905..>3487
                     /gene="deoC"
                     /locus_tag="PLQ54_07225"
     CDS             2905..>3487
                     /gene="deoC"
                     /locus_tag="PLQ54_07225"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010046504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOS93089.1"
                     /translation="MIDHSLLGPALTDAELTAGCELAARYRVATVCIKPYFVSRSVEL
                     LDGSGVKPTTTIGFPHGGHATAIKVAEAAQAMDDGALELDMVVNVGRVLSEDWGFVRS
                     DIGAVADAAHARGVLLKVIFENCFLHDTHKIRLCEICGEVRADFVKTSTGYGEGGATL
                     HDLRLMRENSPAHVQIKAAGGVRTLDGLLAVREV"
BASE COUNT          584 a         1012 c         1215 g          676 t
ORIGIN      
        1 agcttctcca tgacgatctt gtgggtaaag tactgctgca tacccagcga ccgagcgcgc
       61 tgttggattc gctcgttgaa ctccgcttcg gtcatgttct cctcgatgag cgccttttgc
      121 ttggccgctc cttcgccgag actggcggtc tgctgctgga tccactcgag gcgggctttc
      181 tcgatgtcct cgctgctcgc actgagcttg cgccttgcgg catcctgctg cagggccgtg
      241 tcgacgatga gctctttcat gacggccagt tgcatggcgc gctcacgggt cggatcgcct
      301 ttggatcccc catacatctg catctggttc tcgtagttct cgagtgcctg gtggaactcg
      361 gccatgttga tggtcttgcc gttgactctg aaggcgtcgg ggatggagcc catggggttc
      421 cgccccgggc tcccaccgaa gctgaagaag gcgcccactc cgaaaatcgc cgcgatgatc
      481 gcgaggatga tcttggaggc ccttctggag ccggcgctct tctggaaccg aatgaacgat
      541 ggcatgtaca tcctccgcgg cccctggacc catgcaacca acggctcgcg cgaggcgacc
      601 ggaggggggc cagacggcga ggtacgaact gcaccaacca gcacgccagc tcggcggtgc
      661 agactcgcag cgcagttcgg catccatcat aacgcaaacg gagggtgccg tgtagtccgt
      721 ggggccaccg ggccaccgcg ctctggcggc gagcgcagga gtcggcgcag cggcaccgaa
      781 tgataactcc ggataggggg taagccagcg agagggtcag cttggcctcc caccgcatgg
      841 cgttgagtcg cgccgaccgg tgggttttgg gactgagctg ccgttggccg ggtccagccg
      901 gctggcgtgg tgcctcgggg ccggaatcca gttgatgaac gcacgcaacc gcttcctcgt
      961 gtcgacggtg tttgtcagtt tggtgtgtgt ccttggagct gctgaggcct ttgacctttt
     1021 gttcgtcccg ctcgccgaca ccatgcccaa gggaaaagcg gagctacagt cagcgtacat
     1081 tgacaagcct ctcggtgcgc cggctggtgg ggttgttctg aacaacctct actacgggat
     1141 cacggaccgg ctcgagatct ccgcacactc ggcggtgttc atgggtgttc cgggtaaccc
     1201 gccgccgatg ttccaggtca atgccgagta ccgtctcctg gacagcaaga agccgaatca
     1261 cccgacggta tcggtcgggg cgtacaactt gaatggcggt aactttctca actacaatgc
     1321 ggttcagccg tacattgtgg tctcgcacaa tgtgaacatc cccgacaagg gcaagccgga
     1381 atgggacgat ccgctggttc ggctgactgc gggtgtgggt acggcaggac acggcctgtt
     1441 tggcagcgct ctcagcgtgt ttgatccgca cttcgcggcg gaggccggct ggcacggggg
     1501 gcgttaccac gtgtgtgtca actggatgtt gggcgacaag tcacagtacc acgtcacacc
     1561 ggccatctgg aacgggtcgc ctgcactgaa agtggcctat cagcacaagt tctagccaag
     1621 caaggctccg gtcgggacgg acgcggagtg ccctcgcgtg tgccaagcgc gagggctctt
     1681 gttgctttgc ccggacgggg tgcgcggcgg gttgtcaatg atgaggggct ggtatcatga
     1741 cgcaccgcga acggttgctt acggctctcg acctgggtgt gccggatcag gttcccgtca
     1801 cctgggagtt ggtgggacga tgcgcctatg cgcacacggg tgacgccggc tggcgcgggc
     1861 agtgcgacac ccaccgcatg gtcgggtcgt cgattttcaa ccttcagggc gtcgggcctg
     1921 agatcgagtg cgagctgccg gctggattct cggtggagcg cgaggagctg ggcgtcgaag
     1981 acggatggac gctgaccgag acgcgtatcg tgacgccttc ggggacgctg cgggagtgcc
     2041 ggaagaccgg cgggattccc ggtgatccac tgcttgggaa gtgcactcgg tatttcgtgc
     2101 agaacagaga ggactacgag atactcgcca cctacctcga ggctcgggcc cggtgtgcac
     2161 gaccgcggac ccacgagagc agtgtagcgc tggactatgt ccgcgatgat ggtctcgtga
     2221 accactggat gggggattct ctgtacggct tggccaacta tcgcgactcc gaggagttcc
     2281 tggtggatct ggtggagatc cccggtgtgc tgaaggagct gctgcgccac ctgcacgtcg
     2341 agcgagagat agggctggag gcgttcaact cgtcggcggc cgatgtgctg gtgtgggaca
     2401 tctgttggtc ttcgacgagc ctgctcaacc ctcagatggt gcacgagttc gtgctccccg
     2461 aggcgcgctg ggttgtggag aacctggcgc cagggaagcg cgtggttttc ttcacgaccg
     2521 ggcgcatccg cggggtcctt gatgatctgg cccgctgccg gccgcatggg atccagcacc
     2581 tggatgtgct gggtgactgc gatctggccg aagtgaaaca gaccttcggc cgacggttct
     2641 gcctcatggg caactacagc ccggtggtgc tggctcacgg cacgccggag gaagcccaga
     2701 gggaggccaa gcgttgcctc gatgcggcga tggacggcgg cgggtacatc atcaccacct
     2761 cggacgaggt gccggccgac gcccgactcg agaacatgcg ggccgtcgtc gagtacgtgg
     2821 gccgtgaggg acgttacgcg tgagccgtca ggcggacggg agggcccgtt ggacgccgac
     2881 tacacctatg agcagattgc ggggatgatc gatcattcgc tgctgggtcc cgcgctgacc
     2941 gatgccgagt tgacagcggg gtgcgagctg gccgcgcgct accgcgtggc cacggtgtgc
     3001 atcaagccgt acttcgtcag ccggagtgtc gagctgctgg acggcagcgg ggtcaagccg
     3061 acgacgacga tcggtttccc ccacggcggg cacgccaccg cgatcaaggt ggctgaggcc
     3121 gcgcaggcga tggatgacgg cgcgctcgag ctcgacatgg tggtgaacgt tggccgcgtg
     3181 ttgagtgagg actgggggtt cgtgcggtcg gacatcgggg cggtggccga cgcggctcac
     3241 gctcgcggtg ttctgctcaa ggtgatcttc gagaactgct tcctgcatga cacgcacaag
     3301 atccgtctgt gcgagatctg tggcgaggtg cgggcggact tcgtcaagac ctcgacgggc
     3361 tacggcgagg gcggagccac tcttcatgat cttcggctca tgcgcgagaa ctccccggcg
     3421 cacgtgcaga tcaaggcggc gggtggagtc cgaacactcg acgggctgct tgcggtacgc
     3481 gaggtgg
//
LOCUS       DAOSPL010000323         3487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_27060_length_3487_cov_2.711247, whole genome shotgun sequence.
ACCESSION   DAOSPL010000323 DAOSPL010000000
VERSION     DAOSPL010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27060_length_3487_cov_2.711247"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            358..2952
                     /locus_tag="PLQ54_07230"
     CDS             358..2952
                     /locus_tag="PLQ54_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93090.1"
                     /translation="MTTLPLNGTWHVRPDSIDCIGLDGLRKVREAIDGWLPAQVPGEI
                     HLDLIRAGQMPEPTIGAHMPECRWPETRSWWYRRAFEVDERFLSYERQQLVFDGLDLY
                     AQIFVNGRLVGEATNAFVPHAFHVRDLLVAGENELVVRLTAGSETAADATLPGQGQAL
                     QPNTAAGGAIPNPPIDGDPVRARMWPGKKWLRKPQFTYGWDWVDALPNIGIGRGVRLE
                     GRSLAVLDDIRLDTVFHGQQVLLELEAVVENLHPWAERRCVLTVEITAPEGPPPARRE
                     YDLDAPPGRAPVRDWIEILQPRLWWPNGMGEQPLYGVRACTTDADGTVCDERAFHIGL
                     RAIQIDRTRMEEGSRFCIRVNGEDVFCRGGNIGPHDPILARVSDAKYEALVAEARNAN
                     MTMIRINGCSIYEAPAFYDACDRTGILVWQDFMLTCTTYPEHDRSFRDAIQAEARAIV
                     PQLRHHPSIALWCGNNECTWGFRDWWNPDKTQPLDLGGQLFYNQMLPDICRQLDPKRP
                     YWPGSPAGGEDPNDELTGDCHWWHPFFMNADMNRRIRHEVFDECRSRFVSEYGAIGPA
                     HLDSMREYLAPDEMQPGTLAWRLHTSMFEKDTIPAAIRLHYADPEGLDIEHYVRYGQM
                     FQALAHGGAMEALRFRKQDGRDDCAGALIWSYSDCWGETGWSILDYYLRRKASYYAVR
                     RACAPLKVIVRPRGDRLVTRVVNDTLSAVAVTVQTGWWRLDGAARQVESHAIQVPANG
                     MVEAASAPRDPETYDPRHWLYAAVMHHADGTPFDQSIWTLLPHRELALADPVIRVDDT
                     GDGWLRVSSPVYCHAVFTEDGGRELVSENWFDLLPGVPCRTRLADGRTPDEVSFATVP
                     IMPRNPWA"
     gene            2997..>3487
                     /locus_tag="PLQ54_07235"
     CDS             2997..>3487
                     /locus_tag="PLQ54_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93091.1"
                     /translation="MWLLPVVVMLLSAAGAFAAQYSPRVVSPHHADAYSLRTFSEYPR
                     WKDLAGDARAWEIYRYLVDPRTGLFHMNEVIEGDDDLSEYRTIRDPIKIINVYGYAYC
                     AILGPVMAGICEGAGLGEARTLVLPGWSHVAAETFYDGSWHYLDLDVRAAFRRPDGRL
                     ASM"
BASE COUNT          572 a         1231 c         1166 g          518 t
ORIGIN      
        1 ccagccacag caacatggca gtggtgggta gtagcgccga ttacaacatc tcaacggacc
       61 gtgcgaacat ggtcatcccc ctcagtctga gcggcacgaa acatcccctg ttacggttcg
      121 gcaccgccag agacctccgt cggcacgaac gcagagggcg tcggagcccc gcagggcctg
      181 accgagcgga ccacggtccg gtcatgggat ccagccatgg ctcatcggcg agacgcctca
      241 gccccctcgg cggcggacgc ccggcctcgt cagcccggca gggatgccgg cgctcccggg
      301 gaagaccttc cggcggcgcg ctccccgcgc cccagggcct ccagggcagg tgacaccatg
      361 accaccctcc cgctgaacgg cacatggcac gtccgaccgg actcgatcga ttgcatcggt
      421 ctcgacggac tccggaaggt ccgcgaggcc atcgatggtt ggctgcccgc ccaggtgccg
      481 ggcgagatcc acctcgatct catccgcgcc ggccagatgc ccgagcccac catcggcgcc
      541 catatgcccg agtgccggtg gcccgagacc cggtcgtggt ggtaccgaag ggcattcgag
      601 gtcgacgagc gcttcctctc gtacgagcgc cagcagttgg tcttcgatgg cctggacctc
      661 tacgcgcaaa tcttcgtcaa cggccggctc gtcggcgagg cgacgaacgc tttcgtgccg
      721 cacgcgttcc atgtgcggga tctgctggtc gcgggcgaga acgagctggt ggttcggctc
      781 acggcaggaa gcgagacggc ggcagacgca acgttaccgg ggcaggggca ggctctgcag
      841 ccgaacacgg cggccggcgg agcgatcccc aacccgccca tcgacggcga tccagtgcgc
      901 gcccggatgt ggccgggcaa gaagtggctg cgcaagccgc agttcaccta cggctgggac
      961 tgggtcgacg cgctccccaa catcggcatc gggcgcggcg tccgcctcga gggccgcagc
     1021 ctcgccgtcc tggacgacat ccgcctggat accgtgttcc acggccagca ggtgctgctg
     1081 gagctcgagg ccgttgtcga gaacctccac ccgtgggccg agcggcgctg cgttctgacg
     1141 gtcgagatca ccgctccgga gggcccgccg ccggccaggc gggagtacga cctcgatgcc
     1201 ccgccagggc gggcaccggt ccgcgactgg atcgagatcc tgcagccgcg cctgtggtgg
     1261 cccaacggca tgggcgagca gccactctat ggcgtcaggg cctgcaccac cgatgccgac
     1321 gggaccgtgt gcgatgagcg cgccttccac atcgggctgc gggccatcca gatcgaccgc
     1381 acgcgcatgg aggaaggcag ccggttctgc atccgcgtga acggcgaaga cgtgttctgc
     1441 cggggaggca acatcggacc gcacgacccg atcctcgccc gcgtctccga cgccaagtac
     1501 gaggccctgg tcgccgaggc ccgcaacgcg aacatgacca tgatccgcat caacgggtgc
     1561 tcgatctacg aggcgccggc cttctacgat gcatgcgacc gaacgggcat cctcgtctgg
     1621 caggacttca tgctgacctg tacgacctac ccggagcacg accggtcgtt ccgcgacgcg
     1681 atccaggccg aggcacgtgc gatcgtcccg cagctgaggc accacccgag catcgcgctc
     1741 tggtgtggca acaacgagtg cacctggggc ttccgcgact ggtggaaccc ggacaagacg
     1801 cagccgctcg atctcggcgg ccagctcttc tacaaccaga tgctgcccga catctgcagg
     1861 cagctcgacc cgaagcggcc ctattggccg ggcagcccgg cgggcgggga ggatcccaac
     1921 gacgagctca ccggcgactg ccactggtgg cacccgttct tcatgaacgc cgacatgaac
     1981 cgccgcatcc ggcacgaggt gttcgacgag tgccgctcac gcttcgtcag cgagtacggg
     2041 gccattggcc cggcccacct cgactcgatg cgcgagtacc tggctccaga cgagatgcag
     2101 cctggcaccc tggcctggcg cctgcacacc agcatgttcg agaaagacac catcccggcg
     2161 gccatacggc tgcactacgc cgaccccgag gggctggata tcgagcacta cgtgcgctat
     2221 ggccagatgt ttcaggcgct ggctcatggg ggggccatgg aggccctgcg gttccgcaag
     2281 caggacggtc gggacgactg cgccggcgcg ctgatctggt cgtactccga ctgctggggc
     2341 gagaccggct ggtcgatcct cgactactac ctccgccgga aggccagcta ctacgccgtc
     2401 cgccgggcct gcgcgccgct gaaggttatc gtgcgcccac gcggcgaccg gctggtcacg
     2461 cgcgtggtga acgacacgct ctcggccgtg gccgtcacgg tccagaccgg ctggtggcgc
     2521 ctcgacggcg ccgcgcgcca ggtcgagagc cacgccatcc aggtgcccgc gaacggaatg
     2581 gtcgaggcgg cgtcggcgcc acgggacccc gaaacgtacg acccgcgaca ctggctctat
     2641 gcggccgtca tgcatcacgc cgacggcacg ccgttcgacc aatcgatctg gacgctgctg
     2701 ccgcaccgcg agctggctct ggccgacccc gtgatccgcg tcgacgacac cggtgatggc
     2761 tggctgcggg tctcgagccc ggtctactgc cacgcggtgt tcactgagga tggcgggcgc
     2821 gagctggtgt cggagaactg gttcgacctg ctgccggggg tgccgtgccg gactcgcctg
     2881 gccgacggca gaacgcccga cgaggtgtcg ttcgccaccg tgcccatcat gccgcggaac
     2941 ccttgggcct gagcggcccg gatcccgcgg cagtccgacc accgaggtga tccaccgtgt
     3001 ggctcctacc tgtcgtagtg atgcttctct ccgcagccgg cgccttcgcg gcccagtact
     3061 cgccccgcgt ggtgtcgccg catcacgccg atgcctacag cctcagaacg ttctcggagt
     3121 acccccgctg gaaggacctg gcgggcgacg cgcgggcgtg ggagatctac cggtacctgg
     3181 tggacccgcg cacggggctg ttccacatga acgaggtcat tgagggcgat gacgacctca
     3241 gcgagtaccg caccattcgg gacccgatca agatcatcaa cgtctacggc tacgcctact
     3301 gcgccatcct cggaccggtc atggcgggca tctgcgaggg cgccgggctc ggggaggcgc
     3361 gcacgctggt cctccccggc tggagccacg tggctgccga gacgttctac gacggcagtt
     3421 ggcactacct ggacctggat gtccgcgccg ccttccggag acccgacggc cgcctggcat
     3481 cgatgga
//
LOCUS       DAOSPL010000324         3485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_27073_length_3485_cov_2.654227, whole genome shotgun sequence.
ACCESSION   DAOSPL010000324 DAOSPL010000000
VERSION     DAOSPL010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27073_length_3485_cov_2.654227"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..2679
                     /locus_tag="PLQ54_07240"
     CDS             238..2679
                     /locus_tag="PLQ54_07240"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOS93092.1"
                     /translation="MEWHASWIWTAEDPALPNRYMRARRAFVLDDVPSEAVVHCCADS
                     RYCFYVNGHFVGRGPARYHVSTPSYDTYDIAPYLQTGENVLAFLVHHLGCPVFNFMPV
                     RGALIGQAAVQGVDLSTGPEWRVCPAEDYQADVPRLCAQQGYPEIRDTRREPVGWEHP
                     GYDDSGWESATVLGPAALDPWQELEPRGIPHLYEMPLYPESVREVGHCVAPAGMDPMA
                     PGSVAHLVASGHHIGLGSESIRHRCAALAPDERETIIQPGAAGEAVYMVVDFAREVAG
                     YPVLVINGADGAIVDIAYSEALVDGRVDCCRQQLNYADRVILRDGLQTYEPVMPRAFR
                     FMHIEVRNASRPIGLRAVYVSFCGYPVEYYGSFESSDPVLNQIWDIGTYTTQLCMEDA
                     YIDCPWRERGQWWGDARIQALITYYAFGDTRLMRRGLRQIAQSQRPDGLVHACYPAAT
                     DQLIPDFACLWIISLWEHYLYSGDGELLAELMPAVARVVGWFESLIDAHGVLGNVPHW
                     VFIDWADVDKRGEVAALNCFYLGALRHAELIASATGRGEQAAHYHHRAQELRQAIRER
                     FLCPERGALVDCREGEEWSAKIGQQANALAVLFGVFDNPEEASRALEAVREPSPGVVQ
                     VGSPYFSFYLLQALYQQGQHEAALDYIRQRWGAMLDAGATTCWETWRPVWSLCHAWSG
                     GPTYDLSAEFLGVRPLRPGYATFAVRPRCAGVRWMKGIVPSPHGEIAASWVGDPAGDA
                     FTLHSTVPEGTVGRLCIPEITPNHWETIELNGALVWRAGGLGAAKPEVRSAWSERGYI
                     VFELEPGNHTLQARA"
     gene            complement(2688..3404)
                     /locus_tag="PLQ54_07245"
     CDS             complement(2688..3404)
                     /locus_tag="PLQ54_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOS93093.1"
                     /translation="MVGRIAIPPITPQVPHPRLYDLCRRIIGAFCWLCRCEVTGLEHV
                     PATGPVIIASNHQSYADPPLLSRITRRPLSYIAKEELFRIPLMGRFIVWVNAFPVRRG
                     TADLRALRAGLQVLAAGRCLVIFPEGMRSPDGRLLQPELGVALIALKSRAPVLPVALD
                     GSNRVLPRKPPILRPARFRIRVGAPITFEALYDKRHWEREDLEYVARTIMERIAELLG
                     QTFRWEPRGADEGATSSSPE"
BASE COUNT          532 a         1224 c         1166 g          563 t
ORIGIN      
        1 ccagcgctga tcgtcggttc ggcggccgat gttggccctt tcggccggtc tgcttcgccc
       61 cacacctccc cgtgcgccgg gttggggaca tgcggtagcc tgccacggcc cccgccgccg
      121 gctcgctccc cccgggaact gtcaggggca gcgcggcgtt gaccgctcag atggagctct
      181 ggcgggctcg ttcggtgtgc gggcgagcgc ttgagggaac gcgggtgagg cgctgccgtg
      241 gagtggcacg catcgtggat ctggactgcc gaggaccccg ccctacccaa ccgctatatg
      301 cgcgcgcggc gtgcgttcgt gctcgacgac gtaccctccg aagccgtcgt acactgctgc
      361 gcggacagcc gctattgctt ctacgtgaat ggtcactttg tgggccgggg ccccgcacgc
      421 tatcatgtct ccaccccctc ctatgacacg tacgacatcg ctccctacct gcagaccggg
      481 gagaacgtgc tcgccttcct cgtgcaccac ctgggctgcc ccgtgttcaa cttcatgccc
      541 gtccgcggag cgctgatcgg ccaagcagcc gtgcaaggcg tcgatctgag caccggaccc
      601 gagtggcgcg tctgcccggc ggaggattac caggctgatg tgccccggct ctgtgcccaa
      661 cagggctatc ccgagatccg cgacacgcgc cgggagcccg tgggctggga gcacccggga
      721 tacgatgact ccggctggga gagcgccacg gtcttgggac cggccgcgct ggacccgtgg
      781 caggagctcg agccacgcgg cattccgcac ctctatgaga tgccgctcta ccccgagtcg
      841 gtgcgcgagg tcggccactg cgtggcgccc gccggcatgg acccaatggc cccgggttct
      901 gtggcccatc tggtcgcctc gggccatcac atcggactgg ggagcgagtc catacgccac
      961 cgctgtgccg ctctggcgcc ggacgagcgc gagaccatca tccagcccgg cgcggccggt
     1021 gaagcggtct acatggtggt cgacttcgct cgcgaggtgg ccggctaccc ggtgcttgtg
     1081 atcaacggag ccgatggcgc catcgtggac attgcctaca gcgaagcgct ggtcgatggc
     1141 cgtgtggatt gctgccgcca gcagctcaac tacgccgacc gggtgatcct gcgcgatggg
     1201 ctccagacct acgagccggt catgccccgg gcgttccggt tcatgcacat cgaggtccgc
     1261 aatgcgtcca gacccatcgg cctacgcgcc gtgtacgtga gcttctgtgg ctatcccgtc
     1321 gagtactatg ggagcttcga gagctcggac ccggtgctga accagatctg ggacatcggc
     1381 acctacacga cccagctctg catggaggac gcgtacatcg actgcccctg gcgggagcgg
     1441 ggccagtggt ggggcgatgc gcggatccag gcgctcatca cctactacgc tttcggcgat
     1501 acccggctga tgcggcgcgg gctccggcag atcgctcagt cgcagcggcc cgacgggctc
     1561 gtccatgcct gctacccggc ggccaccgac cagctcatcc ccgatttcgc ctgcctgtgg
     1621 atcatctcgt tgtgggagca ctatctgtac tcgggcgatg gcgagctcct ggccgagctc
     1681 atgccggcgg tggcgcgagt cgtcgggtgg ttcgagtccc tcatcgacgc tcacggcgta
     1741 ctcggcaacg tgcctcactg ggtgttcatc gactgggccg acgtggacaa gcgcggtgag
     1801 gtcgcggccc tcaactgctt ctacctcggt gcgctccggc acgccgaact catcgcctcg
     1861 gccacgggcc ggggcgagca ggcggcccac taccatcacc gggcacagga gcttcgccaa
     1921 gccatccgcg agcggttcct gtgtcccgag aggggggctc tggtcgactg ccgtgagggc
     1981 gaggaatgga gcgccaagat cggccaacag gccaacgccc tggcggtgct cttcggcgtg
     2041 ttcgacaatc ccgaagaggc gtcgcgcgcg ctcgaagccg ttcgcgaacc ctcgccgggc
     2101 gtggtgcagg tggggtcgcc ctacttctct ttctacctcc tgcaggccct ataccaacag
     2161 gggcagcacg aggcggcact ggactacata cggcagcgct ggggggccat gctcgacgcg
     2221 ggagccacca cgtgctggga gacctggcgg cccgtgtgga gcctctgcca cgcgtggtcc
     2281 ggcgggccca cctatgacct gtcggccgag tttctgggcg tccgtcccct tcgcccgggc
     2341 tatgccacct tcgcggtgcg cccccgctgc gccggggtca ggtggatgaa gggcatcgtg
     2401 ccctcgcccc acggtgagat cgccgcctcg tgggtgggcg atccggccgg ggacgcgttc
     2461 acgctccact ccaccgtgcc cgagggcacc gtgggacggt tgtgcattcc ggagatcacg
     2521 cccaaccatt gggagaccat cgagctcaac ggagctctgg tctggcgagc tggcgggctg
     2581 ggcgccgcca agcccgaggt gcggtcggcc tggtccgagc ggggctacat cgtgttcgag
     2641 ctcgaacccg ggaaccacac gctccaggcc cgcgcctgac accaccgcta ctccggagac
     2701 gacgatgtgg cgccctcatc cgcgccgcgc ggctcccacc ggaacgtctg cccgagcagc
     2761 tcggcgatgc gctccatgat cgtccgagcc acatactcca ggtcctcgcg ctcccagtgc
     2821 cgcttgtcgt agagcgcctc gaaggtgatg ggcgccccca cccggatgcg gaaccgggcc
     2881 ggccgcagta tgggcggctt gcgcggcagc acgcggttcg agccatcgag agccaccggc
     2941 aggaccggcg ctcgcgactt gagcgcaatc agcgccactc ccagctccgg ctgcaacagg
     3001 cggccatcgg ggctccgcat cccctcgggg aagatcacca gacaccgccc ggccgccagc
     3061 acctggaggc ccgcacgaag cgcgcgcagg tccgccgttc cccggcgcac ggggaaggca
     3121 ttcacccaca cgatgaaccg ccccatcaag gggatccgga atagctcctc cttggcaatg
     3181 tagctgagag gccgccgggt gatgcgcgac agcaaggggg gatcggcata gctctggtgg
     3241 ttcgaggcga tgatcaccgg ccccgtggcc ggaacgtgct cgagccccgt cacctcgcat
     3301 cgacacaacc agcagaacgc cccgatgatc cggcggcaca ggtcgtacag ccgcggatgg
     3361 ggcacctggg gggtgatcgg tgggatcgca atcctaccca ccacccgcac ccgccttcac
     3421 tgccaaggaa gtgaccgcgt cgaccacctc gtcgaccggc atcccgtcag tgaggatctc
     3481 gacgg
//
LOCUS       DAOSPL010000325         3477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_27146_length_3477_cov_3.172122, whole genome shotgun sequence.
ACCESSION   DAOSPL010000325 DAOSPL010000000
VERSION     DAOSPL010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27146_length_3477_cov_3.172122"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1455)
                     /locus_tag="PLQ54_07250"
     CDS             complement(16..1455)
                     /locus_tag="PLQ54_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOS93094.1"
                     /translation="MLSTCLSLAASLALAPLLPGAVSVRDHGAVGDGVSDDTDAFQAA
                     IEEAAGTGAAVIVEPVEPGKGYVLTHTVTLKQGVSLIGSLAGMPFIAWEGVPREIQTG
                     PVILARPRASEYEDQRKAPLLRLEGGNTLRGLYILYDQQPWPSDAEFDAPGSPYAYAS
                     LEELTQRFIPDHVAPCGPTIEIRPGVASTTVEDITCARYRDFLYTAAGGKIVVSRCYL
                     FGYGRAFAIREAKDVVRISEIHIVPNVEGPISWQHAKLQAAITSSPANTAFDLGSVDG
                     YSISDVAVFLCHTGFRLGASEASPFVDPVTGELVTHQWGLGPWGSMHNIKLDNCVVGF
                     DCINGTILPNQMTNLMVHASINTPARFAAAEGDLARQAAFLIEPGFVGATLQIANLSL
                     SSFAPRNVVATGRMVAEANGRAFLIDCPPADRVDFAVRNTAQVEVFGLAISNIPETHF
                     VAAAAGTTPSIRVRGFVHNGVPKDDGPLR"
     gene            1862..>3477
                     /locus_tag="PLQ54_07255"
     CDS             1862..>3477
                     /locus_tag="PLQ54_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93095.1"
                     /translation="MRVVIACIAMTAPAALAQPSNCRFYIAGQYDFGGRLGFYLDFEG
                     TELVRLPLILGVADGTNWRFLSHAPGFAPDRDYTIRVVLAPDRAELWVDGERVGSSPG
                     TWRAAPVPLEANQRPSWATERGDWIAIVSNLVVSVERNGTTADRREFDFGDIAARPVA
                     LQLVEEGRPSSGPLETRDGDTLTIEVTLRFGDADLTRYAPFIDRYGQYRYADFPGKVR
                     SDDDLRADIASEDDRLEAMPPPDDRDEYGGVTGFWTEAPTGFFRVVQRDGVWWLISPR
                     GNPCFLTGVSGPPAQLWEKTPTTGREFLFEWLPPRDGPYGVAWAADVWGEGQGTDYVC
                     LHTANLIRKYGDDWQAKSSERTVRRLRAWGFVCGKWGAPVTEVQTPVLHLGATPRIDR
                     HPDVFDDAILATMREELEKQIAPRRDDPRIVGWSVGNEYDEIITPAEVRAILAKPESA
                     ACGALVRHALEVLHAGSVAKLAEAWQVDAADLAGVLAGDPTVPDVDLEALRRVYADRY
                     YRALYETIKGIDPNHLYLGNWIVPGWWVNE"
BASE COUNT          534 a         1163 c         1211 g          569 t
ORIGIN      
        1 cgttcgggtc gccccctacc gcagcggccc gtcgtccttg ggcacgccat tgtggacgaa
       61 ccctcgcacg cggatcgagg gcgttgtgcc tgccgcggcg gcgacgaagt gtgtctctgg
      121 tatgttggag atcgccaggc cgaacacctc gacctgagcc gtgttgcgaa cggcgaagtc
      181 cacccgatcc gccgggggac aatcgatcag gaaggcccgg ccgttggcct cggcgaccat
      241 tcgcccggtg gcgaccacgt tccgcggcgc aaacgagctg agcgacaggt tcgcgatctg
      301 gagtgtcgcg ccgacgaaac ccggctcgat caggaaagcc gcttggcggg ccaggtcccc
      361 ttcggccgcc gcgaaccggg ccggagtgtt gatgctcgcg tgcaccatga ggttggtcat
      421 ctggttcggt aggatggtgc cgttgatgca gtcgaagccg acgacacagt tgtcgagctt
      481 gatgttgtgc atcgagcccc acggaccgag gccccactgg tgggtcacca gttcgccggt
      541 cactggatca acgaatgggc tcgcctccga tgcgccgagc cggaacccgg tgtggcagag
      601 gaacaccgcc acatcgctga tcgagtagcc atcgacggag cccaagtcga acgctgtgtt
      661 ggcggggctt gacgtgatgg cggcctgcag cttggcatgt tgccacgaga tgggaccctc
      721 gacattgggc acgatgtgga tctcggagat gcggacgacg tcctttgctt cgcggatggc
      781 gaacgcccgc ccatacccga acaggtagca gcgggagacg acgatcttcc cgccggcggc
      841 ggtgtacagg aagtcgcgat agcgtgcaca ggtgatgtcc tcgacagttg tcgaggccac
      901 gcccggccgg atctcgatgg tgggaccgca gggcgccacg tgatcgggga tgaagcgctg
      961 cgtcagctcc tcgaggctgg cgtaggcgta gggcgagccc ggcgcgtcga actcggcatc
     1021 ggagggccat ggctgctggt cgtagaggat gtacagacca cggagtgtgt tgccgccctc
     1081 gagccggagc agcggagcct tgcgttggtc ctcgtattcg gaggcgcgcg gccgcgccag
     1141 gatgaccgga ccggtctgga tctcgcgggg gacaccttcc cacgcgatga agggcatccc
     1201 cgccaggctc ccgatgaggc tgacgccctg cttcagggtg accgtgtggg tgagcacgta
     1261 gcccttgccg ggctccacag gctcgacgat cacggcggcg ccggtcccag ccgcttcctc
     1321 aatggccgcc tggaaggcgt cggtgtcatc gctcacgccg tcgccgacgg cgccgtgatc
     1381 gcggaccgac acggcaccgg gcaacagcgg cgccagggca aggctagcgg cgagcgacag
     1441 gcatgtgctg agcatggggg ctccttccgc ggcacgcgtc tttgcggttc agcgggccca
     1501 tggcgggttc ctgcgaaccc cgccgccacg cgccgagggg gcaggagtcg gcggtaccac
     1561 tggtggcccg ggggccggag cgtgggggcg gtgcggcttt ccccacccga ggctccatac
     1621 cacattgccg gtcgggtgga tacgcgttca ggagcccctg ccgccgagtc gtgccgtggc
     1681 ggtgcctcat acgcgttcct gccggacgcc gatccatcgg ccctgagccg gtcgaagggc
     1741 ggcccctcga acccttgccc gctgccaggt cctcggccgt cgcgggccga atgccctcac
     1801 gcctggccga cgtctccatc catgcagtcg ggcttgagcg ccaagtaccg ggaggcaacc
     1861 catgcgagta gtcatagcct gcattgccat gacggccccg gccgcgctcg cgcagccgtc
     1921 gaactgccgg ttctacatcg cgggtcagta cgacttcggc ggcaggctcg ggttctacct
     1981 cgacttcgag ggcacggaac tggttcggct acccctgatc ctcggcgtcg ctgacggcac
     2041 caactggcgt ttcctgtccc acgctcccgg cttcgcaccg gaccgcgact acaccatccg
     2101 cgttgtgctg gccccggacc gcgccgagtt gtgggtcgac ggcgaacggg tcggctcgag
     2161 cccgggcact tggcgtgcgg cgccggtgcc gctcgaggcc aaccagcgtc cctcttgggc
     2221 caccgagcgg ggggactgga tcgccatcgt ctcgaatctc gtcgtctccg tggagcgcaa
     2281 cgggactacg gccgatcggc gcgagttcga cttcggcgac atcgccgccc ggccggtggc
     2341 cctccagctg gtcgaagagg ggcgcccctc ttccggccca ttggagactc gcgatggcga
     2401 tacgctcacg atcgaggtca ctctccggtt tggtgatgca gacctcacgc gctatgcgcc
     2461 cttcatcgac cgctacggtc agtaccgata tgcggacttc ccgggcaagg taagatccga
     2521 tgacgacctg cgggccgaca tcgccagcga ggatgaccgg ctcgaagcca tgccgccccc
     2581 ggacgatcgc gatgagtacg ggggcgtgac ggggttctgg accgaggcgc ccaccggctt
     2641 cttccgcgtg gttcagagag acggcgtgtg gtggctcatc agcccgcggg gcaatccgtg
     2701 ctttctgaca ggtgtcagcg gccccccggc acagctgtgg gagaagaccc ccaccaccgg
     2761 tcgcgagttc ctgttcgagt ggctcccgcc ccgcgatggg ccttacggcg tcgcctgggc
     2821 cgcggacgtc tggggagagg gccagggcac cgactatgtc tgtctccaca cggccaatct
     2881 catccgcaag tacggcgatg actggcaggc caagtccagc gagcggaccg tccggcgcct
     2941 gcgggcctgg gggttcgtgt gtggcaagtg gggcgctccg gtcacggagg tgcagacacc
     3001 ggtgttgcac ctgggggcca cgccccggat tgaccggcac cccgacgtgt tcgacgacgc
     3061 catcctcgcc accatgcggg aggagcttga gaaacagatc gcgccgcggc gggacgaccc
     3121 ccgcatcgtc ggctggtctg tgggtaacga gtacgatgag atcatcaccc ccgccgaggt
     3181 ccgggccatc ctcgcgaagc ccgaatcggc ggcctgtggc gcgctggtcc gccatgcgct
     3241 cgaggtcctc catgccggct cggtggccaa gctggccgag gcgtggcagg tcgacgccgc
     3301 cgacctggct ggcgttctgg ccggcgaccc cacggtgccc gatgttgacc tcgaggccct
     3361 gcgccgcgtc tacgctgacc gctactaccg ggcgctgtac gagaccatca agggcatcga
     3421 cccgaaccac ctgtacctcg gcaactggat cgtccccggg tggtgggtca acgagga
//
LOCUS       DAOSPL010000326         3474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_27188_length_3474_cov_2.899386, whole genome shotgun sequence.
ACCESSION   DAOSPL010000326 DAOSPL010000000
VERSION     DAOSPL010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27188_length_3474_cov_2.899386"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..813)
                     /locus_tag="PLQ54_07260"
     CDS             complement(202..813)
                     /locus_tag="PLQ54_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93096.1"
                     /translation="MPRPPNADFFDLREIALFRPGGDVEWKRAWSARVEQAGLRMSLT
                     TDGKYWGVAASDYPDRPFSTWVIVGDARDGRVLWQTPDTRAPGLHVQFSILGAAEAVI
                     VSPVEGGCTGFYGKDGAVVLLPGPSGQENSAGDRVYVLAGVEPEGSCLLVCYDIYGQP
                     LWRMRDARPRATAPGGLGGFALSPSGEWLADLGVDPAAIYRAR"
     gene            complement(950..1660)
                     /locus_tag="PLQ54_07265"
     CDS             complement(950..1660)
                     /locus_tag="PLQ54_07265"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOS93097.1"
                     /translation="MHTWVLLACLALPAAPAVPVDGLVAEFQFEEGGARRPVDASGLG
                     QQARSAGVERVRGHGGWGLRFAEAGSVVRVASRPSLSLDRAITLEAWVLPGPISDESR
                     VIIAKNDEYLLRIDKASEGGRVSFFVHVGSPGVTWEPRVSSVGPISPGVWHHIAGTWD
                     GRKLRLYVDGDAQGEVDRSGSPNPNPYPVMIGNFEYPSCHGGAFGGAIDEVRIYQRAL
                     RPEEVRQGAREGQPQPAE"
     gene            1880..>3474
                     /locus_tag="PLQ54_07270"
     CDS             1880..>3474
                     /locus_tag="PLQ54_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOS93098.1"
                     /translation="MSDTMSRRTFIRGMGAAAASALAGRAHAQTREATVGNRPNVLFI
                     MTDQQRADTIAALGNDRIYTPNLDRLVRRGVSFTNAYTTCPVCVAARLTIRTGRQPRR
                     TGVYGNSPPTLVDGEPLAPEERCGPYLARTMRGLGYRTFGIGKFHSMPWDEDLGYDTQ
                     LHSEELYGSPDQRGRDAYAAFVAREHPEYDFIEGLMGERTEMYYMPQMSPMPKECTVE
                     AWAADRAVESIGADDDRPYFGFVSFVGPHPPLAPPIPFNRMYDPDRMPNPIRGDRDTD
                     LADEQIRWMNYAIWAEDINDPHARVLRARYYGEVSYIDWCLGRILDAVDARSDADNTV
                     ICFFSDHGDHLGDHHAWQKESYFEQSCRIPYLVSWPARLPQDTRRADLVCHSDLFGIA
                     TGAAGARDTRDGVDLLGAIEDGAPTRETLVGTYSDPGTPEFKTMVRHGHWKYIYMANG
                     AREQLFDLAEDPGELRELSARHRSTTRRLRELAVDYLAGSPNSARALDGADLLGFPPT
                     ERPLQRIYQFDYSRGVRGFPESP"
BASE COUNT          575 a         1268 c         1151 g          480 t
ORIGIN      
        1 ccccaaccct ccccgcggac ggggagggag ccggctcctc acctcccccg ttcgcggggg
       61 aggccgggag agggcttccc ctcctacaag cccaggacga gtgcgcacca gcccggcgca
      121 cgtcatcgtc agcctccgac ctagccgatg ccgggcgacg gcgagccaga ccgcccacct
      181 cacgcggcag cccccgcccc tctaccgcgc cctgtagatg gcggccggat ccacacccag
      241 gtcggcgagc cactcgcccg agggcgacag cgcgaatccg ccgagcccgc ccggagcagt
      301 ggctcgcggc cgcgcgtcgc gcatgcgcca gaggggctgc ccgtagatat cgtagcacac
      361 cagtaggcag ctgccctcgg gctcgacacc ggcgagaacg tacacgcggt cgcccgccga
      421 gttctcctgg cccgacggtc ccgggagtag gacgactgcc ccgtctttcc cgtagaagcc
      481 cgtacagccg ccctcgacgg gcgacacgat gaccgcctcg gccgcgccga ggatgctgaa
      541 ctgcacgtgc aggcccgggg cgcgtgtgtc cggtgtctgc cagagcacgc gcccgtcacg
      601 agcgtccccc acgatcaccc acgtcgagaa ggggcggtct ggatagtccg atgcggccac
      661 gccccagtac ttgccgtcgg tcgtgagcga catgcggagg ccggcctgct ccactctggc
      721 cgaccaggcc cttttccact ccacatcgcc gcccgggcgg aagagggcga tctcgcgcag
      781 gtcgaagaag tcggcattcg ggggacgcgg caacggcgtc acatcgcggg gctccgcccc
      841 gccccccgcg cgaggggcca gcccctcgcg gctcccatcc ggctgccgcc cgaccggcga
      901 tggccctgcc agagcatgag ggcggccgtc ccgctacgcg gcccgcccct cactccgcgg
      961 gctgcggctg cccctccctc gccccctggc gcacctcttc tggacgcagc gcccgctggt
     1021 agatccgcac ctcgtcgata gcgcctccga acgccccgcc gtggcagctc gggtactcga
     1081 agttgccgat catgacgggg tatgggttcg ggttggggga gcccgaacga tctacttcgc
     1141 cctgcgcgtc accgtcgacg tacaagcgca gcttgcgtcc gtcccacgtg ccagcgatgt
     1201 ggtgccatac gccgggcgag atggggccca cgctggacac cctcggctcc cacgtcacgc
     1261 cggggctccc gacgtggacg aagaaggaga ctcgcccgcc ctcggaagcc ttatcgatgc
     1321 gcagcaggta ctcatcgttc ttggcgatga tgacgcggga ctcgtcggag atggggccgg
     1381 gcaggaccca cgcctcgagg gtgatggccc tgtcgagact caggctcggg cgcgacgcga
     1441 cccggacgac ggatccggcc tcggcgaagc gcagccccca cccgccgtgc ccgcgcaccc
     1501 gctcgacgcc ggcggaacgc gcctgttgcc cgagcccgga cgcgtcgacg ggtctgcgcg
     1561 ctccaccttc ctcgaactgg aactcggcca ccaggccatc gaccgggaca gccggggctg
     1621 ccggtagcgc caggcaggct agcagcaccc acgtgtgcac gggccacacc tccagatagt
     1681 ggcatgatac ggcggccgag ccgacaagcc tgcctgggaa cacaggccag gcgcctctcg
     1741 tctcgcggtg ccggaaaggc aggtagcggc gacgcccttc cgccagccca cgccacggaa
     1801 ccaggcgtcg ctcgttccca cggcgaagca gcccgcgcgg ctgggcccca agggcccgac
     1861 gcgataggga gttctggaca tgagcgacac gatgagccgc cggaccttca tcaggggaat
     1921 gggcgcggcc gccgcctcgg ctctggcggg gcgcgcccat gcccagacaa gggaggccac
     1981 agtgggtaac cggcccaatg tgctcttcat catgaccgac cagcagcgcg cggacaccat
     2041 cgccgcgctg gggaatgacc ggatctacac accgaacttg gaccgtctgg tccgccgcgg
     2101 cgtgtccttc accaacgctt acacgacatg cccggtctgc gtcgcggcgc gattgaccat
     2161 ccgaacgggc cgccagccgc gccgtaccgg cgtctatggg aactcacctc cgacgctggt
     2221 ggatggcgag ccgctcgctc cggaggagcg ctgcgggccg tacctcgccc ggaccatgcg
     2281 gggtctcggc taccgcacgt tcgggatcgg caagttccac tcgatgccgt gggacgagga
     2341 cctcgggtat gacacccagc tccacagtga ggagctctac ggttcaccgg accagcgcgg
     2401 ccgcgatgcc tacgcggctt tcgtcgccag agagcacccg gagtatgact tcatcgaagg
     2461 cctaatgggc gagcgcaccg agatgtacta catgccccag atgagcccca tgcccaagga
     2521 gtgcaccgtc gaagcctggg cggccgaccg ggccgtcgag agcatcggtg ccgacgacga
     2581 caggccttac ttcggcttcg tgtccttcgt cggcccgcac ccgcctttgg caccgccgat
     2641 accgttcaac cgcatgtatg accccgaccg gatgccgaac ccgatcaggg gtgaccgcga
     2701 caccgatctg gcggacgagc agatccgctg gatgaactac gcgatctggg cggaggacat
     2761 caacgacccg cacgcgcgcg tgctgagagc acgctactac ggcgaggtgt cctacatcga
     2821 ctggtgcctg gggcggatcc tcgatgccgt cgacgcacga tcggacgcgg acaacacggt
     2881 catttgcttc ttcagcgacc atggtgacca cctgggcgac caccatgcat ggcagaaaga
     2941 gagctacttc gagcaatcgt gccggatccc gtatctcgtg agttggccgg cccggctccc
     3001 gcaggacact cgccgtgcag acttggtctg tcacagtgac ctgttcggga tcgccaccgg
     3061 cgccgcgggg gcacgggaca cgcgggacgg cgtggacctg ctcggtgcca tcgaggacgg
     3121 ggcgcccacg agggagacgc tggtcgggac gtatagcgac ccagggacac cggagttcaa
     3181 gaccatggtt cggcacggcc actggaagta catctacatg gccaacggcg cgcgcgagca
     3241 gctcttcgac ctggcggagg accccggtga gctgagggag ctatcggcgc gccaccggag
     3301 cacgacgagg cgcctgcgtg agctggccgt cgactacctg gcgggatcgc ccaactccgc
     3361 tcgggcgctc gacggcgcgg acctgctcgg ctttcccccc accgagaggc ccctgcagcg
     3421 gatctaccag ttcgactact cgcgcggcgt tcgagggttc ccagagagcc ccga
//
LOCUS       DAOSPL010000327         3461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_27317_length_3461_cov_3.272754, whole genome shotgun sequence.
ACCESSION   DAOSPL010000327 DAOSPL010000000
VERSION     DAOSPL010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27317_length_3461_cov_3.272754"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2059)
                     /gene="mutS"
                     /locus_tag="PLQ54_07275"
     CDS             complement(<1..2059)
                     /gene="mutS"
                     /locus_tag="PLQ54_07275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOS93099.1"
                     /translation="MSAPDSANLTPMFRQWEAAKAEYPDALVLFRMGDFYELFGEDAE
                     VASRILDLTLTSRECGQRNKIPMCGVPFHSVDRYLGRLIDQGLKAAICEQIGDPKLAR
                     GLVERRITRVITKGTAIEDFLLEARANNYLAALYRDPADTRHIGVAWADVSTGEFEAC
                     EVNGGLVEAVEELGRLCPSEVVLDESLAAEDGVSDSLAGRLGARVIAAKADPHGRRGR
                     QTLCRHFGVASLAGFGLEDAAAACEAAAIVLEYLRESKLDVANHLRAISLRAPGERMY
                     LDAATRRNLELTHSIRGGGREGTLLTLIDLTLTPMGSRRLRQWLLAPLRAVEPILARQ
                     EAVASLAQAAGVRRHIREALERVGDLERLMARIGTGSASARDLVALGRSLERLPAIRA
                     AIEGQTGKKLLELSQALDAVPEVSSAIRSALVDEPPVSLREGGLIRPGHCADLDELRD
                     VASSGKDWIAALEAQERDRTGIKSLRVGFNSVFGYYIEVSNANRDLVPEDYIRKQTLA
                     NAERYITPGLKEYESKVLGAEEKINALEYELFLALRQHVAGFAGRVLDSAAALAEVDA
                     LAALAEVAVRRNYVRPEVDDGDAIEIEDGRHPTVESLMPEGEFVPNDCRLDSLRQQMA
                     IVTGPNMAGKSTYLRQVALTVLLAQIGSFVPARRARLGVVDRIFTRVGAHDDLAQGQS
                     TFMV"
     gene            complement(2046..3398)
                     /gene="miaB"
                     /locus_tag="PLQ54_07280"
     CDS             complement(2046..3398)
                     /gene="miaB"
                     /locus_tag="PLQ54_07280"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOS93100.1"
                     /translation="MRRIHVVTYGCQMNVHDSEMILGVLQAAGHQVAPSIEEADVVLL
                     NTCCVREKPERKVLSKVAELKRMREQQRPELIIGVCGCMAQSLQEELFSLAPAVDLAS
                     GPDEIGRLPELVAAVEAGERHVSAMRLGHTRPASDLPKARSDDLQAWVTIMHGCTNRC
                     SYCIVPDVRGPERSRPIGEIREEVAQLVASQVREVTLLGQNVNAYGMDLPPGSPRFPR
                     LLAELDGIAGLARIRFTTSHPKDFGDDLIEAVAGLPRVCEHVHLPVQAGSDEVLARMN
                     RRYTRDQYLALVDRIRAAIPDVSITTDVMVGFPGETDAQFEQTLDVFERVRFDQAFMF
                     IFSPRRGTRAERMADPVPREVKRRRLERLSARQNEIAREKNQTHVGRAFEVLVEGPSA
                     RDPSRLSGRTRTNKIAVFGPLPGRASPARGRFVWVRVTRGYLWGYEAEVIDTACHECS
                     "
BASE COUNT          588 a         1148 c         1151 g          574 t
ORIGIN      
        1 cgaccatgaa ggtcgactgc ccctgagcca agtcatcatg ggcgccgacg cgagtgaaga
       61 tgcggtcgac cacgcccagt ctggcccgcc gggcgggtac gaagctaccg atctgggcca
      121 gcagcacggt cagggccacc tggcgcaggt aggtcgactt gcccgccatg ttggggccgg
      181 tgacgatggc catctgctgc cgcagggagt cgagccggca gtcgttgggc acgaactcac
      241 cctcgggcat gaggctttca acggtgggat ggcgcccgtc ctcgatctcg atggcgtcgc
      301 cgtcgtcgac ttcgggccgg acgtagttgc gccgcacggc gacttcggcc aacgccgcca
      361 gcgcatccac ttcggccagg gctgcagctg agtcgagcac ccggccggcg aaccccgcca
      421 catgctggcg cagggccaga aacagctcgt actcgagcgc gttgatcttc tcctcggccc
      481 caaggacttt cgactcatac tccttgaggc cgggcgtgat gtaccgctcg gcattggcca
      541 gggtctgctt tcgaatatag tcctcgggca ccaggtctct gttggcattg ctcacctcga
      601 tgtagtaacc gaacaccgag ttgaagccca ctcgtaacga cttgatgccg gtgcggtcgc
      661 gttcttgagc ctcgagcgcc gcgatccagt ccttgccgct gctggcgaca tcacgcagtt
      721 cgtcgaggtc ggcacagtgg ccgggacgga tcaggccccc ttcgcggagc gacacgggcg
      781 gctcgtcgac cagggccgag cggatggccg aggagacctc gggcactgca tcgagggcct
      841 ggctgagctc caggagcttc ttgccggttt ggccctcgat cgccgcgcgg atagccggga
      901 gccgttcgag agaccttccg agtgccacca ggtcgcgggc cgatgccgag ccggttccga
      961 tgcgcgccat cagacgctcg aggtcaccga cgcgctcgag cgcttcacga atgtggcggc
     1021 ggacgccggc ggcctgggcc agactcgcca cagcctcctg ccgcgccagg atcggctcga
     1081 cggcccgcag cggcgccagc agccactggc ggagccgccg cgagcccatg ggggtgaggg
     1141 tcaggtcgat cagcgtcaac agcgtgccct cgcgaccgcc gccccggatc gaatgggtga
     1201 gctccaggtt gcgtcgtgtg gcagcgtcga ggtacatccg ctcgccaggc gctcgaagcg
     1261 agatggctct gaggtggttg gccacgtcga gcttgctctc acggaggtac tcgagcacga
     1321 tggcggccgc ctcgcaggct gccgcggcgt cctcgagtcc aaatcctgcc aatgaggcga
     1381 cgccgaagtg ccggcatagc gtctggcggc ctcgccggcc gtgtgggtcc gctttggccg
     1441 cgatcacacg cgcacccagc ctgcccgcaa gactatccga aacgccatcc tcggcggcca
     1501 ggctctcgtc gagcacaacc tcagatggac acagccgccc gagctcctcg accgcctcga
     1561 caagcccccc gttgacctca cacgcctcga actcgccggt ggacacatcc gcccatgcca
     1621 ctccgatgtg gcgtgtatca gcgggatcgc ggtagagcgc tgccaggtag ttgttcgccc
     1681 gcgcctcgag caggaagtcc tcgatggcgg tgcccttggt gatcacgcga gtgatgcggc
     1741 gctcgaccag gcctctggcg agtttaggat caccgatctg ctcacagatg gcagctttga
     1801 gtccctgatc aatcagccgg cccaggtagc ggtcgaccga atggaagggc acgccgcaca
     1861 tgggaatctt gttcctctgg ccacactcgc gagacgtcag ggttagatcg aggatccgag
     1921 aggccacctc ggcgtcctca ccaaagagct cgtagaagtc gcccatacga aagagcacca
     1981 gcgcatcggg atactcggcc ttggcggcct cccactggcg gaacatgggc gtcaggttgg
     2041 cggaatcagg agcactcatg gcaagctgtg tcgatgacct ccgcctcgta gccccacagg
     2101 tagccgcggg tgacccggac ccagacgaag cgaccgcgcg cggggctggc gcgccccggg
     2161 agcgggccaa acaccgcgat cttgttcgtg cgcgtgcgtc cagacagccg tgaagggtcc
     2221 cgtgcactgg ggccttcgac cagcacctcg aaggccctgc ccacatgagt ctggttcttc
     2281 tcgcgggcga tttcgttctg ccgggccgag aggcgctcca agcggcgtct cttcacctct
     2341 cgcggcaccg ggtcggccat ccgctcggcc cgagtgccgc ggcgcgggga gaagatgaac
     2401 atgaaggcct ggtcgaatcg cacgcgctcg aagacatcca gcgtctgctc gaactgagca
     2461 tcggtctctc ccgggaaccc gaccatcacg tccgttgtga tcgatacatc aggtatggca
     2521 gcccggatgc ggtcgaccaa tgccaggtac tggtcgcggg tgtagcgccg gttcatacgg
     2581 gcgaggactt catcggaacc ggcctggacc ggcaggtgaa cgtgttcgca gaccctaggc
     2641 agccccgcca cagcctcgat gaggtcatcg ccaaagtcct tgggatgcga ggtggtgaac
     2701 cggatgcgcg ccaggccagc gatgccatca agctcggcga gcaaccgggg aaaccggggg
     2761 gacccggggg gcaggtccat cccgtacgcg ttgacgttct ggcccaggag ggtcacctcg
     2821 cgcacttggg aggccaccag ttgcgctact tcctcgcgaa tctcgccgat gggccggctc
     2881 cgctcgggcc cgcggacatc ggggacgatg cagtagctgc aacggttggt gcagccatgc
     2941 atgatcgtca cccaagcctg taggtcgtcc gaccgcgctt tgggcaggtc gctagccggc
     3001 cgcgtgtgtc ccaaccgcat ggcggagacg tgtcgctccc ccgcctcgac ggcggccacc
     3061 agctcgggca gacggccgat ctcgtcgggc ccgcttgcca ggtcgacggc gggcgccagg
     3121 gagaacagct cctcctgcag ggactgggcc atgcatccgc agactccgat gatgagctcc
     3181 ggcctctgct gttcgcgcat ccgcttgagt tcggcgacct tactgaggac cttccgttca
     3241 ggcttctcgc ggacgcagca ggtgttgagg agcacgacat cggcctcctc gatagagggg
     3301 gccacctggt gtccggcagc ctgaagtacc ccgaggatca tctccgagtc gtggacgttc
     3361 atttggcagc cgtaggtgac gacgtggatg cgtctcatag ggaagaaggg gcgggaccga
     3421 tcagcctcta gcaaccgatt ataggaagcg cgacgacggc g
//
LOCUS       DAOSPL010000328         3461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_27319_length_3461_cov_3.083676, whole genome shotgun sequence.
ACCESSION   DAOSPL010000328 DAOSPL010000000
VERSION     DAOSPL010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27319_length_3461_cov_3.083676"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2372
                     /locus_tag="PLQ54_07285"
     CDS             <1..2372
                     /locus_tag="PLQ54_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93101.1"
                     /translation="RFRDPAGRVIYLGRATDLRSRVRSYAGDLGARRHLRRMVPQVAG
                     VEALVCASVPEAAWLERNLLERSLPRWNRIRGGTELVGWLVVTDDPARPGLDLVVGEE
                     QPTGPAFGPYLGVDRLAVARDALLRAWPVPLTGTRIDRAGRALAEARGVTDNEREDYL
                     GKVRAVLARDAAATATLHALLGEASDLESQFLCLTLDRERRALYGCAANPARIYRADM
                     GQALEGTLESAVFDATAQARWTSLEYTADMPEGTEVTVEVRSGGTAVPDATWSTWSRP
                     YSKPDRQRLDRPDGRYCQYRATLRSQGGGALPVLKAVRVSYLKHNARPTIAFDKEIPR
                     AASGELELSWKAEDPNGDKLTYSIDISRDGGRTWKTAKSDIEESTAKWDTSGEEDGTI
                     VLRVVASDAKRNPTDPLTNEAISKPMILDNSGPTVSLLKGNIQPIGNGAIRVRAWAED
                     TGSGVGSAQYRVDEGKWVALPAEDGLFDSSYEALEFDIKDLSGGEHTIQVAAFNAAGK
                     EASASVKCTVEGPPAEGKPADSDVVSTADTAATGDAKSGDETTGEPPADEGAEAGPSL
                     PVATEEPAHGPVPEAPADAAELPSGRDIVARTLERSRGIEDFTVRGRGRLRLPKGASF
                     NGQSGTYKGDFVGRFKRPDKIATQGDPVGSLFETVLAFVAPETIIEEDDRVKVLGVEE
                     VDGTEADLVWIEDPDGDQARLWISRADSSILKLVLSSVVEPGSRSRIEMKFTWTSVND
                     MPVPMTVEVVLPPEFVEGARGPVSAALTLTGWQINTGLNDELFPKADDE"
     gene            complement(2383..2871)
                     /locus_tag="PLQ54_07290"
     CDS             complement(2383..2871)
                     /locus_tag="PLQ54_07290"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOS93102.1"
                     /translation="MGANESDLRRLEFVERAQFGSCFIIRIPPGENLIRRIQELLAAK
                     EARRAAILSGIGSLRDVTFRNLAEGVELPVNKDKTIDTKREGPFELVSLVGNLIPMLG
                     RPVTHLHAVLGTPDGQLVGGHLFDATVFDTVELVVAEIAGSRVVKGRDESTGLAEMKI
                     AE"
     gene            complement(3057..3133)
                     /locus_tag="PLQ54_07295"
     tRNA            complement(3057..3133)
                     /locus_tag="PLQ54_07295"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3097..3099),aa:Met,seq:cat)
     gene            complement(3157..3330)
                     /locus_tag="PLQ54_07300"
     CDS             complement(3157..3330)
                     /locus_tag="PLQ54_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93103.1"
                     /translation="MATANIQRLRGFSADRFLALERQLFEAVVRAEEARKRAIEEAKA
                     KKRKSKKKKRRAA"
BASE COUNT          613 a         1155 c         1181 g          512 t
ORIGIN      
        1 accggttccg cgacccggcc ggcagggtga tctacctcgg ccgcgcgacg gatctccggt
       61 cgcgggtgcg ctcctatgcc ggtgacctcg gcgcccgcag gcacctgcgg cgcatggtgc
      121 cgcaggtcgc aggggtcgag gccctcgtct gtgcctccgt gcccgaggcc gcctggctcg
      181 agcgcaacct gctggaacgc tcgctgccgc gctggaaccg gatccggggc ggcacggaac
      241 tggtcggctg gctggtggtg acggacgacc cggcccggcc agggctcgac ctcgtcgtcg
      301 gcgaagagca gcccaccggc ccggccttcg gcccgtacct cggtgtcgat cgcctcgccg
      361 tggcccgcga cgccctgctg cgcgcctggc cggttcccct caccggcacc cgtatcgacc
      421 gcgccggccg cgcgctggcc gaggcacgcg gcgtgacgga caacgagcgc gaggactacc
      481 tgggcaaggt gcgggctgtg ctggcccgcg acgcggcggc gacagcgacc ctgcacgcgt
      541 tgctcggcga ggccagcgat ctcgagagcc agttcctgtg tctcactcta gaccgggaga
      601 ggcgggcgct ctacggatgt gcggccaacc cggcgaggat ctaccgggcg gacatgggac
      661 aggctctgga aggcacgctc gagtccgccg tgttcgatgc caccgcccag gcacgatgga
      721 ccagcctcga gtacacggcg gacatgcccg aaggcaccga agtgaccgtg gaggtgcgct
      781 ccggcggcac cgccgtgccc gacgcgacct ggagcacgtg gtcgaggccc tactcgaaac
      841 ccgaccggca gcggctcgat cgtcccgatg ggcgttactg ccaataccgc gcgacgctga
      901 gaagccaggg cgggggcgcc ctgcccgtgc tcaaggccgt ccgggtctcg tacctcaagc
      961 acaacgccag accgaccatc gccttcgaca aggaaatccc ccgtgccgct tcgggcgaac
     1021 tcgagttgtc ctggaaggct gaggatccca acggggacaa gctgacgtac tccatcgaca
     1081 tctcgcgcga cggtgggcgg acatggaaga cggcgaagtc cgacattgag gaatccactg
     1141 ccaagtggga cacctcaggc gaggaggacg gcaccatcgt cctgcgtgtg gttgccagcg
     1201 acgccaagcg aaaccccacg gatccgctga caaacgaagc catcagcaag ccgatgatcc
     1261 tcgacaacag cggccccacg gtgtcgctgc tcaaggggaa catccagccc atcggcaacg
     1321 gggcgattcg tgtccgggcg tgggccgagg acaccgggtc cggtgtcggc agcgcccaat
     1381 accgggtcga tgagggcaag tgggtagctc tccctgcgga agacggcctg ttcgactcca
     1441 gctacgaggc attagagttc gacatcaagg acctgtcggg cggcgagcac accatccagg
     1501 tggccgcctt caatgccgcc ggtaaggaag cgtccgcgtc ggtcaagtgc accgtcgaag
     1561 gcccacccgc cgaggggaaa ccggccgaca gcgatgtggt gtccacggca gacacggccg
     1621 ccaccggaga tgccaagagc ggggacgaga cgaccggcga gccgccggcc gatgaggggg
     1681 ccgaagccgg tccttcgctg cccgtggcca cggaggagcc cgcccatggg ccagtgcccg
     1741 aggcgcctgc cgacgcggca gaactcccct ccggccgcga catcgtggcg aggacactgg
     1801 agcgcagcag gggcatcgag gacttcactg tcagggggcg tggacgactg aggctgccca
     1861 agggggccag cttcaacggc cagtcgggca cctacaaggg cgacttcgtt gggcggttca
     1921 agcgcccgga caagatcgcg acccaagggg atcccgtggg ctcgctgttt gagacggtgc
     1981 tggccttcgt ggcccccgag accatcatcg aggaggacga ccgcgtcaag gtccttggcg
     2041 tcgaggaggt cgatggcacc gaggccgacc tggtatggat cgaagatccc gacggcgacc
     2101 aagcgcggct ctggatcagc cgagccgatt catcgatcct caagctcgta ctatcgtcgg
     2161 ttgtcgaacc cggaagccgc tcgcggatcg agatgaagtt cacctggacc tcggtgaacg
     2221 atatgccagt cccgatgacg gtcgaggtcg tcctgcctcc cgagttcgtc gagggcgccc
     2281 ggggtccggt cagcgccgcg ctcacgctca cagggtggca gatcaacacc ggcctgaacg
     2341 acgagctgtt cccgaaggca gacgacgagt agcgagcgga agctactcgg cgatcttcat
     2401 ctcggccaga cccgtggact cgtcgcggcc cttcacaacg cggctgccgg caatctcggc
     2461 gacgacgagt tccaccgtgt cgaagacggt ggcgtcgaac aggtgccctc ccacgagctg
     2521 gccgtctggc gtgccaagca cggcatggag atgagtgacc gggcgcccga gcatcgggat
     2581 caggttcccg acgaggctca ccagctcgaa aggcccctcg cgcttggtgt cgatggtctt
     2641 gtccttgttc accggtagct cgacaccctc agcgaggttg cggaaagtca catcgcggag
     2701 cgaaccgatg ccggagagaa tcgcggcccg gcgggcctcc ttggcggcga gcagttcctg
     2761 gatccggcgg atcagattct caccgggcgg gatgcggatg atgaagcagc tcccgaactg
     2821 ggcacgctcg acgaactcca gtcggcgcag gtcggactcg ttggctccca tccagcgctc
     2881 cttccgggca atgccggtcg gctgaccgag tcgctggtgg cgcgacccgg cacccataga
     2941 acgccaacac cggcttcgcg gatcatgcgg gccggactgg gtggtgccgc gacgcgtaca
     3001 aaacaacaca cccggctcgc gtcgagcggc gcctggtgcg ttcgcgaggg gaaggatggt
     3061 agcaggggtg ggattcgaac ccacgacgtc acgggtatga accgtgtgct ctgaccaact
     3121 gagctaccct gccggaatgg ggctgccccg gaggggctat gccgcccttc gcttcttctt
     3181 cttggactta cgcttcttgg ccttggcttc ctcgatggcc cgcttgcgag cctcttccgc
     3241 gcggaccaca gcctcaaaga gctgacgctc gagggccagg aaccgatcgg cactgaaacc
     3301 acgaaggcgc tgaatgttcg ccgttgccaa ccaaatcacc ggggacagag tgtagcattc
     3361 gatacgtccg gttgtcaaac accggcgcga ttccgcgatt cctcctacgg gcccgcctgg
     3421 gccgcggcgg gggcgcggaa ccaacaccgg tcggactcat a
//
LOCUS       DAOSPL010000329         3459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_27337_length_3459_cov_3.367509, whole genome shotgun sequence.
ACCESSION   DAOSPL010000329 DAOSPL010000000
VERSION     DAOSPL010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27337_length_3459_cov_3.367509"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3412
                     /locus_tag="PLQ54_07305"
     CDS             <1..3412
                     /locus_tag="PLQ54_07305"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS93104.1"
                     /translation="DLSTHGRGVGTFWKGSFYGISEAHTYKFSDVGMACGSLGRAAAR
                     ALGVAPELSVRDTVNTGAERHWSTFLPLALCLEGEAATLAGYSGITLASIEDARPYLD
                     TPFDTPERVDFANLALQARAICATVPNLLADVSVPGLFTADPGNGFALVHGGVFEFGR
                     SISFLPQDKVPGAIAAWLRQPKSVGGVRRTVLDEVDEKSQYRFAGMVNQSHRGGGDEL
                     GAFHCDPETGDIDYAADYGPTGVKALGFDNFAVASNDAGKDIVVFRCCPLEIYDIFDP
                     ASFSHLSQVKVLDRQTNATPFDWGRIVPSFQSFEPAAVVFGKPGKHLIVTLRPGGLYA
                     RMLVLNGTDDNPLGNGMRMGQDRAVFYGAYSAVKDLWRLDETRINKFSAWGVGDKRAL
                     QLHAETAALIAEAEQARDAGRIDVFIATSRKALAIENRVYPKVEGTARDVVNGVLFYL
                     VLLVPFSYLAERLLVAARTLKWQAAWTFIIFAIVFLTLVCVHPAFRVTTTPVVVLLAF
                     ILAGMAVLVTLLVIRHFDQLMEDFRYKKSGQHTADVARLSTAGSAFALGVANMRRRTL
                     RTSLTSITLILLTFTVLSFTAVTTSQTVRAIRQSYPPAYDGLLLRTPNLGAFAENTYV
                     ALRNEIDPQYPMAPRAWLLSVGSGNESVVPIWHGDQRYQATALLGCHADEPKVTPVLQ
                     TLVGPRSRWFNGSQAKRFNIGEDDVGTATVSINGYQLRVIGVFDESAMDAIKDLDDEQ
                     LMPVDEIREANLRKSGESSRKEETSQDPSALQTRVRIPSQSVVLVPYDLCLRCGGPLR
                     SISVKFGAGDVARQAQDDMVYRLDLSSLSGYDGQVYYRTSIAAAKVSGLEGSLVPMLI
                     AALIVLNTMLGSVYERIKEIGIYSSLGLSPAHVGFLFVAESAVYSVLGAMVGYVLGQG
                     AAKIVVANPEWSKYLSLNYSSMAAVGSTLIIMAVVFLSTLYPARRAADLAAPGVERRW
                     RLPQPDGDSLRVRLPFTVARAEAAGCFLYVGEYLDAHSDYTLGEFSTDGCAYVPPTDG
                     GVGHRLVFTAWLSPYDLGVSERVVLETVETPEEEVLGIDALVFRVSGDEGAWLRTTRS
                     FVYLLRRQFLLWRVLGPELKASYHEQAAAAAKGAGSTERVGASGERGE"
BASE COUNT          565 a         1133 c         1144 g          617 t
ORIGIN      
        1 ggatctgtcc acccacggcc gcggcgtcgg caccttctgg aagggcagct tctacggcat
       61 ctccgaggcc cacacataca agttttcgga tgtggggatg gcctgcggca gcttgggcag
      121 ggcggcagcc cgggccctgg gggtcgcccc cgagctctcg gtgcgggaca cggtcaacac
      181 cggtgccgag cgccactggt cgacttttct gccgctggcg ctctgcctcg aaggcgaggc
      241 agccacactg gccggctact cagggataac tctggccagc atcgaagatg cccggcccta
      301 cctcgacacg cccttcgaca cacccgagcg cgtcgacttc gcgaacctgg cgctccaggc
      361 cagagccatc tgcgcgaccg tgccgaacct gctggccgat gtgagtgtgc ccgggctgtt
      421 cacggccgac ccagggaacg ggttcgcgct ggtccatggc ggcgtcttcg agtttgggcg
      481 cagcatctcg ttcctgccgc aggacaaggt gccgggcgcc atcgcggcat ggctgcgaca
      541 gcccaagtcc gtggggggtg tgcgccgcac ggtgctggat gaggtcgacg agaagagcca
      601 gtaccgcttc gccggcatgg tgaaccagtc gcatcgtggc gggggagacg agctgggcgc
      661 cttccactgc gacccggaga ccggcgacat cgactacgcc gcggactatg ggccgaccgg
      721 tgtcaaagcg ctcggtttcg acaacttcgc cgttgcctcc aacgatgccg gcaaggacat
      781 tgtcgtgttc cggtgctgcc cgctggagat ctacgacatc ttcgatcccg ccagcttcag
      841 ccacctgagc caggtgaaag tcctcgaccg gcagaccaac gccacgcctt ttgactgggg
      901 ccgcatcgtg ccgagcttcc agagcttcga accggccgcc gtggtcttcg ggaagccggg
      961 caaacatctg atagtgaccc tgcgccccgg tgggctctat gcccgcatgt tggtgctcaa
     1021 cggcaccgac gacaacccgc tgggcaatgg catgcggatg gggcaggacc gcgccgtgtt
     1081 ctatggtgcc tactcggccg tgaaggatct gtggcggctc gacgagacac gcatcaacaa
     1141 gttctccgcc tggggcgtgg gcgacaagag agccctccag ctccatgcgg agaccgccgc
     1201 gctcatcgca gaggcagagc aggcgcgcga cgcgggccgg atcgacgtgt tcatcgctac
     1261 ctctcgcaag gccctggcca tcgagaaccg ggtctacccc aaggtggagg gtacggcacg
     1321 cgatgtggtc aacggcgtgc tgttctacct ggtcttgctg gtgccgttct catacctggc
     1381 ggagcgcctc ttggtcgcgg cacgcacgct caagtggcag gcggcatgga cgttcatcat
     1441 cttcgccatc gtcttcctca cgctggtctg cgtgcacccg gctttccggg tcaccacgac
     1501 gccggtggta gttctcctcg cattcatcct ggcaggtatg gcggtgttgg tgactctgct
     1561 cgtcatccgc cacttcgatc agctcatgga ggacttccgg tacaagaagt ctgggcagca
     1621 cacggctgac gtggcgcggc tcagcacggc gggatcggcg ttcgcgctcg gtgtggccaa
     1681 catgcggcgc cgcacgctcc gaacctcgct cacctcgatc accctgatcc tgctcacctt
     1741 taccgtgctg tccttcaccg cggtgaccac cagccagacg gtccgggcca tccggcagag
     1801 ctaccccccg gcgtacgatg gcctgctgct acgcacaccg aaccttgggg ccttcgccga
     1861 gaacacgtac gtggcgttgc ggaacgagat cgacccgcag taccccatgg cccctcgcgc
     1921 ctggctcctc tccgtcgggt ccggcaatga gtccgtcgtg ccgatctggc atggtgacca
     1981 gagataccag gccaccgccc tgctcggctg ccatgccgat gagcccaagg tcaccccggt
     2041 gctgcagaca ctcgttggcc caaggagccg gtggttcaac ggttcgcagg ccaagcggtt
     2101 caacatcggc gaggacgatg tcggcacggc cacggtcagc atcaacggat accagctccg
     2161 tgtgatcggg gtcttcgacg agtcggccat ggatgccatc aaggacctcg acgacgagca
     2221 gctcatgccc gtagacgaga tccgcgaggc caacctgcga aagagtggcg agtcgagcag
     2281 gaaggaggag accagccagg acccatcggc cctccagacg cgagtccgca tcccctcgca
     2341 gtcggttgtg ctggtgccct acgacctgtg ccttcggtgt ggcggtcccc tgcgatccat
     2401 tagcgtgaag ttcggcgcgg gcgatgtggc ccggcaggcg caggatgaca tggtctatcg
     2461 gctcgacctg agcagcctgt cgggatacga cggccaggtc tactaccgca cgtccatcgc
     2521 ggcggccaag gtcagtggtc tcgaaggcag tctcgtgccg atgctcatcg ccgcgctcat
     2581 cgtcctcaac accatgttgg ggagtgtgta cgagcgcatc aaggagatcg gtatctacag
     2641 ctcgctcggg ctctcgcctg cccatgtggg cttcctgttc gtggccgaga gcgccgtata
     2701 ctcggtgttg ggcgccatgg tcggctatgt gctgggccag ggcgccgcca agatcgtggt
     2761 tgcgaacccc gagtggagca agtacctgag cctcaactac agctcgatgg ccgcagtggg
     2821 ctcgacgctg atcatcatgg ccgtggtgtt cctctcgact ctgtacccgg cccgcagggc
     2881 cgccgacttg gccgctccgg gggtcgaacg tcgttggcgt ctgccgcagc ccgatggcga
     2941 ctcgctacgc gtgcgcctcc ccttcacggt ggcccgcgcc gaggccgccg ggtgcttcct
     3001 ctacgtgggc gagtacctgg acgctcactc cgactacacc ctcggcgagt tctcgactga
     3061 cggctgtgcg tacgtgccgc ccaccgacgg cggggtgggg catcggctgg ttttcacggc
     3121 atggctgtct ccctatgatc taggagtgag tgagcgagta gttcttgaaa cggtggagac
     3181 gccggaagag gaggtcctcg gcatcgacgc actcgtgttc cgggtcagcg gcgatgaggg
     3241 agcctggctg cggaccactc ggagcttcgt ctacctgctg cgtcggcaat tcctgctgtg
     3301 gcgcgtcctc gggccggagc tcaaagcgag ttaccacgag caggccgcgg ccgcggcgaa
     3361 gggcgcgggg agcaccgaac gcgtcggggc cagcggggag cgaggtgagt agccatggcg
     3421 gagcgcgcac aggccgacct tgaggagtat cggcaactg
//
LOCUS       DAOSPL010000330         3457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_27356_length_3457_cov_4.329806, whole genome shotgun sequence.
ACCESSION   DAOSPL010000330 DAOSPL010000000
VERSION     DAOSPL010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27356_length_3457_cov_4.329806"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PLQ54_07310"
     CDS             <1..609
                     /locus_tag="PLQ54_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93105.1"
                     /translation="YTVRDVQRLVTGEAREFEFLRATAVPVRVVYRINQWAYNGQPRR
                     VLVVRNDPALGLGLAPLPGGHVWLLAPGDATAGPLLEGDLAQIAPGQEAELDTGPSRL
                     VTARRTLMNYSLASLQYDPTGAISGYDRQEEYRLELRSVSGSPVAVEVFEDIPGVWTL
                     RTAEAFSKVNDSTVVFPVTLAPGELRTLSFTIVRREGTRVRG"
     gene            606..1742
                     /locus_tag="PLQ54_07315"
     CDS             606..1742
                     /locus_tag="PLQ54_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93106.1"
                     /translation="MNGAARDVRWVARVVAAAVVALAAGTVYASVSLTSQAQRGPIRL
                     LLTAPPRALVIEQRTVQLAPGPNRLTVQWDRARVDRASVFLRAPEAGDGIDLSGAIFP
                     RGMDNAVCWDVVATRAQPVTLEALYRLDGLTWAPEYRIEMTAGAEQGAFRATALLANN
                     TGDDLPSAPYFVGDQCIGEQPLANGETVRLGFFERPALALAPQTVFDASRYGNAVVLR
                     ARLTNTAESGLGCAALHRGRVRTYLRTEEGALALSGEDELPDLPIGCTADVFLCYQDD
                     LRATRSELPSTDVNVRRDSRGRTVVYDSDQNRRIELENPRPHPVAIRVIEHADGQWQI
                     GRSTHAYTRTDATTLEFTVDVPAAGETNGKAVLEYRLRRQNLAP"
     gene            2079..2651
                     /locus_tag="PLQ54_07320"
     CDS             2079..2651
                     /locus_tag="PLQ54_07320"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOS93107.1"
                     /translation="MAGLRDRVRPLDDRLRVRVRAALCDAIEYAAQVCDVPLALVALM
                     DRGTVWFTALALMEDDETSRCGQLCMQSIMGREPLIVGDTLLDERFATNPLVARAPAI
                     RFFAGFPILSDGQRVIGSLNVLDLRPRQLTDEQVRGLQTLGRLLAVQLDILRSALAEA
                     DLEDEALGSPEPSGSWARAPDADRRGGSHM"
     gene            2743..>3457
                     /locus_tag="PLQ54_07325"
     CDS             2743..>3457
                     /locus_tag="PLQ54_07325"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HOS93108.1"
                     /translation="MNILSRLSVKTKLLGLVILLIIAMGAIGWTGMSGIKQLNAQSAA
                     IQEDCVQPSLHALEASTIAATWYKDLVGHSLTNDMKLKKDKYEPEMKQFEADAETTLR
                     LIIDSKANVSKETREGATAALEAFGKLVPIKEKVIALSEQLKDQEAFNVMLTEMRDPM
                     TELTGGLEELAKKTQDEIKSAQKNAEAKAASVTKMAIIVTAVAAVIGLLVGLVISFSL
                     SRGIGAVVDGMQELAAGNLT"
BASE COUNT          595 a         1112 c         1177 g          573 t
ORIGIN      
        1 tacacggtgc gagacgtgca gcgcctggtc accggcgagg cccgcgagtt cgagttcctg
       61 cgcgccacgg cggtgcctgt cagggtggtg taccgcatca accagtgggc ctacaacggg
      121 cagccccggc gcgtgttggt tgtgaggaac gatccggctc tggggctcgg actggccccc
      181 ctgcccgggg gccatgtgtg gctcctggca cccggcgatg cgacggccgg cccgctgctc
      241 gagggcgatc tggcccagat cgcgcccggc caggaggcgg agctcgacac cgggccgagc
      301 cgcctggtca ctgccagacg caccctgatg aactactcgc tggcgagcct gcagtacgac
      361 ccgacgggcg ccatctccgg ctatgaccgc caggaggagt accgcctgga gctccgcagc
      421 gtgtcgggta gtcccgtggc ggtcgaggtg ttcgaggaca ttccgggtgt ctggacactg
      481 aggaccgcgg aggccttcag caaggtcaac gacagtaccg tggtgttccc agtgacgctg
      541 gctcccggcg aactacggac gctgagcttc accattgttc ggcgcgaggg aacgagggtg
      601 cgcggatgaa cggagccgca agagacgtga gatgggtcgc gcgtgtggtc gctgccgcgg
      661 tcgtggctct cgcggcgggg accgtatacg cctcggtatc gctgacgagc caggcgcagc
      721 ggggcccgat ccggctgctt ctgacggcgc cgcctcgggc tctggtgatc gagcagcgaa
      781 cggtccagct ggcgcccggc ccgaatcgcc tgacggtaca gtgggaccgg gctcgcgtgg
      841 accgggcttc ggtcttcctg cgcgcgcccg aggcgggtga tggcatcgac ctcagtggcg
      901 ccatcttccc gcggggcatg gacaacgccg tgtgctggga cgtggtcgcg acgcgcgcgc
      961 agccggtgac gctcgaggcc ctctaccggc tcgatgggct gacctgggcg cccgagtacc
     1021 gcatcgagat gaccgccggg gcggagcagg gcgcgttccg tgccacggca ctgctggcca
     1081 acaacacggg tgatgatctg ccctcggccc catacttcgt gggcgatcag tgcatcggcg
     1141 agcagccgct ggccaacggc gagacagtga ggctcgggtt cttcgagcgg ccggctctgg
     1201 ccctggctcc tcagacggtg ttcgatgcga gccggtatgg caatgccgtg gtgctacgag
     1261 cgcggctgac taacaccgcg gagagcgggc tcgggtgcgc ggcgctccat cggggcagag
     1321 tgcggaccta tctgcggacc gaggaaggtg ccctagccct gtcgggcgag gacgaactgc
     1381 cggacctgcc catcgggtgc acagccgatg tgtttctgtg ctaccaggat gatctccgag
     1441 ccacgcgttc cgagttgccc tcgactgacg tgaacgttcg gcgcgacagc cgggggcgaa
     1501 cggtggtcta tgactcagac cagaaccgcc ggatcgagct cgagaacccg cggccacacc
     1561 cggtggcgat ccgggtgatc gagcatgccg atgggcagtg gcagatcgga cggtcgactc
     1621 acgcctacac ccggaccgat gcgacgacgc tggagttcac cgtcgacgtg ccggcggccg
     1681 gcgagaccaa tggcaaggcc gtcctggagt accgcctcag gcgacagaac ctggcgccat
     1741 agggcacagg ctccccctgg gcggtcattg ggggctccag tggcccgagg ttggggcgtg
     1801 cggatcgggc gcgttggggg cccaccacgc tgggggacgc gtggttcccc ccatggcgcc
     1861 cctccgcaac caggctgatg ggccacggga gccctcagtt accgccaatg cccctatccg
     1921 gcccgttctg caccgggaat cctcacccgc cccatcaggg aattcccatg cccagttccg
     1981 ggattccccc gataacgcgt tcgcccattt cccggcaccc tgttgtctgg tgaccggccc
     2041 gtggggccgg caccagattc cgaggggcgg gatcgggaat ggccgggctt cgtgaccgtg
     2101 tcaggcctct agatgaccgc cttcgcgttc gtgttcgtgc cgccctgtgt gatgccatcg
     2161 agtatgccgc acaggtctgc gatgtgcccc tggccctggt cgcgctgatg gatcgaggca
     2221 ccgtgtggtt tacggcccta gccctcatgg aagacgatga gacctctcga tgcgggcagt
     2281 tgtgtatgca gagcatcatg gggcgagagc ccctcatcgt gggcgacacc ctgctcgatg
     2341 agcgcttcgc gacgaacccc ttggtggcac gcgcccccgc gatccgattc ttcgccggct
     2401 tccccattct ctccgatggc caacgggtca tcgggagcct caacgtgttg gacttgcgtc
     2461 cccggcagct cacggacgag caggtccgag gccttcagac cttgggccga ctcctggctg
     2521 tacagctcga catcctccgt tccgcccttg ccgaggcgga tctcgaggat gaggcattgg
     2581 gctccccgga gcccagcgga tcctgggcac gcgctccgga tgcggacagg agaggggggt
     2641 ctcacatgtg aggaccccac tcaagttcac cgacctcccc tgagctctga gagctcgaac
     2701 tgagcgatct ccccgtaagc ccgctggaaa ggcgtggtga cgatgaacat cctgtcccgg
     2761 ctctcggtca agaccaagct tctcggactc gtgatcctac tgatcatcgc catgggggcg
     2821 attggttgga ccggaatgag cggcatcaag cagctcaatg cccagagcgc ggctatccaa
     2881 gaagactgtg tccagccgtc gcttcatgct ctggaagcct cgacgatagc ggcgacctgg
     2941 tacaaggact tggtcgggca ctctctgacc aacgacatga agctcaagaa agacaaatac
     3001 gagcccgaaa tgaagcagtt cgaggcggat gcggaaacga ccctcaggct catcatcgac
     3061 tccaaggcga acgtcagtaa ggagacgcgc gaaggggcaa cggccgccct cgaagcattc
     3121 ggcaagctgg tacccatcaa ggagaaggtc atcgcactct cggagcagtt gaaggaccaa
     3181 gaagcgttca atgtgatgct cacagagatg cgcgacccca tgaccgagct taccggcggg
     3241 cttgaagagc tggccaagaa gacgcaggat gagatcaagt ctgcccaaaa gaacgccgag
     3301 gccaaagccg ccagcgtcac caagatggcc atcatcgtaa ccgccgtggc ggcggtgatc
     3361 ggcctgctcg tcggcctggt tatcagcttc agtctgtcgc ggggcatcgg cgccgtggtg
     3421 gacggcatgc aggagctggc cgcgggcaac ctcaccc
//
LOCUS       DAOSPL010000331         3456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_27374_length_3456_cov_3.193767, whole genome shotgun sequence.
ACCESSION   DAOSPL010000331 DAOSPL010000000
VERSION     DAOSPL010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27374_length_3456_cov_3.193767"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PLQ54_07330"
     CDS             complement(<1..557)
                     /locus_tag="PLQ54_07330"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HOS93109.1"
                     /translation="MPEIDRKHVAILRVLERAERPLGAGKVAKELLSHGVELTERAVR
                     YHLEYMDRLGLTEPMGRPGRRITDAGRRELAEARVADKSSLVFAHLEAFAYETTFDVE
                     TGRGRVAVNVSLLPADAGRQALRVMRPVLNSRMATSRRIRIYQPGEWVGNQVVPRDSV
                     GIGTVCSVTINGILLKRGIPVQSLFG"
     gene            783..2867
                     /locus_tag="PLQ54_07335"
     CDS             783..2867
                     /locus_tag="PLQ54_07335"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOS93110.1"
                     /translation="MHRSAYPPQLVRGRALAAGLVLLVLMLESSEGAGGPVQVFGDVL
                     PRLADRRFTQVRDGNTAYTLSVPVSVAQWEARAAELREHILACTGLWPPPEPAPLNAE
                     VFGRIERDGYSIEKVYFESFPGFYVTGNLYRPLGRTGPFPGVISPHGHWARGRIHDED
                     LGSIPGRCITMARQGYVVFAYDMIGYNDSDQVAHSIGGEAEYLWGISAMGLQLRDSIR
                     AVDFLRSLPDVDPARIGCTGASGGGTQTFMVAAVDDRIVAAAPVNMISAHFQGGCICE
                     NAPNLRIDTFNVEIGALMAPRPLLMVSCTGDWTANTPKVEFPAIQSVYRLLGAEDRVG
                     WVQIDAPHNYNQASRLAVYKHLGKWLLGEDDESKLGEPPFGLEPDEAMLVWAGHERPA
                     GALDQRGVIEALKAQARAQIAASVPRDAKELAALPGSLGVTLAHSLNVTRPAPGDVRA
                     ESLGPVTTGSLRGDRLVLGSTSRGDAVPAVAWMPPRGPRSGRAALVVAERGIASLLGQ
                     GGDPRPLLRGLLDAGALVLAIDAFGTGASIAANPASQREGVKFFTTFNRTDTAERVQD
                     VLTAIAYLQSRVDVDRVDLVGLEKAGLWCLLARAFAPSVSRAALDLDGFPGTDEAYLS
                     DLYVPLLLRAGGVQTAGALAAPGALLLHHVSPDLDLSMTRAAYAAADAGANLRAERER
                     LSVSEVLRWLNR"
     gene            complement(2874..>3456)
                     /locus_tag="PLQ54_07340"
     CDS             complement(2874..>3456)
                     /locus_tag="PLQ54_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93111.1"
                     /translation="KGNHVSIIVAGRRVTEADMRAFIASPRVRELLPSAASHGDCFRG
                     AFPAGRARGIFSERCIIVGDAAGMVRPFKGGGIRAALLTGRAAGRALLEFGPCSQAGA
                     SYVAACGDLASDVAYGRLLRALVALLSGPFRLEAVIALARHSPELQRALYDCVSGRTS
                     YRQVFREQLTPRLLSRIAWDCLTWPLRSRGPGR"
BASE COUNT          525 a         1149 c         1217 g          565 t
ORIGIN      
        1 ccgaacagtg actgcacggg aataccccgc ttgagaagaa tcccgttgat cgtcacgctg
       61 catacggtgc cgatgcccac cgagtcacga gggaccacct ggttgccgac ccattcgccc
      121 ggttggtaga tccggatacg gcggctggtc gccatgcgcg agttgagtac ggggcgcatc
      181 acacggagcg cctgacgccc cgcgtcggcc gggagtagcg acacattgac ggccactcgc
      241 ccgcggccgg tctcgacatc gaacgtcgtc tcatatgcga aagcctcaag gtgggcgaac
      301 acgagactgg acttatcggc cacgcgggct tcagccagct cgcggcgtcc cgcgtcggtg
      361 atccggcggc cggggcgtcc catgggctcg gtgagtccga ggcggtccat gtactcgagg
      421 tggtagcgga ccgcccgctc ggtcagctcg acgccatggc tcagcagctc cttggcgacc
      481 ttgccggctc cgagagggcg ttcggcacgt tcgagtaccc tcaggattgc tacatgcttc
      541 cggtcaattt ccggcaagcc gtgatcctcc tcacctggcg tctgagagat gatgtcattc
      601 tacttctgca ttcggagcgt ttcaacggca gcggtgtccg gatgggtctg tcgcggaagg
      661 gcctggtctg ttgacggatg gttgccggta atggattgcc gtcactggag tccaagagtc
      721 tcgggactgc cggcgaggca cgggacccgc gcgggccgaa taggggggtg gaggtgggcc
      781 cggtgcatcg ctcggcgtat cccccacagc tcgttcgggg ccgtgccctc gcggccggtc
      841 tggtcctgtt ggtactcatg ctcgaatcgt cggagggagc gggtggcccc gtgcaggtct
      901 ttggtgatgt tctgccgcgc ttggcggacc gccgtttcac tcaggttcgc gacggaaaca
      961 cggcctacac cttgagcgtg ccggtgtcgg tcgcccagtg ggaggcccgg gccgccgagc
     1021 tccgcgagca catactcgcg tgcacggggc tgtggccacc gcccgagccg gcgccgctca
     1081 acgccgaggt cttcggccgc atcgagcgcg atgggtacag catcgagaag gtctacttcg
     1141 agagcttccc gggcttctac gtcaccggca atctgtaccg tcccctgggc cgtacggggc
     1201 cgttccccgg ggtcatcagc ccgcacggac actgggcccg gggccgcatc cacgatgagg
     1261 accttgggtc gattcccggc cgctgcatca ccatggcacg gcagggctac gtggtctttg
     1321 cctatgacat gatcggctac aacgacagcg accaggtcgc ccacagcatt ggcggcgagg
     1381 ctgagtacct ctggggcatc tcggccatgg gacttcagct gcgggacagc attcgagccg
     1441 tggacttcct gcgctcactt cccgacgtcg atccggcccg gattggctgc acgggggcgt
     1501 ccggaggggg tacgcagacg ttcatggtcg cggccgtcga tgatcgcatc gtggctgccg
     1561 cgcccgtgaa catgatttcg gcgcacttcc agggcggctg catctgcgag aatgccccaa
     1621 acctccggat cgacaccttc aacgtcgaga tcggcgccct gatggctccg cgcccgctgc
     1681 tgatggtgag ttgcacgggg gactggacgg cgaacacgcc caaggtcgag ttccccgcca
     1741 tccagagcgt ctaccgcctg ctcggagccg aagaccgcgt gggctgggtc cagatcgatg
     1801 cgccgcacaa ctacaaccag gccagccgtc tggccgtcta caagcacttg ggcaagtggc
     1861 tgctcggcga ggacgacgag tcgaagctcg gcgagccccc gttcggtctc gagccggacg
     1921 aagccatgct cgtgtgggcc ggccacgaga gacccgcggg ggcgctcgat caacgggggg
     1981 tcattgaggc cctcaaagct caggccagag cgcagattgc cgcgtcagtg ccccgcgacg
     2041 ccaaggagct cgccgcgctg cccgggagcc tgggggtgac gctggcccac agcctgaacg
     2101 tgacgcgacc ggccccgggc gatgtgaggg ccgagtcgct ggggcctgtg acgactggat
     2161 cgctccgcgg cgaccggctg gtgcttggat ccacctcccg gggcgatgcg gtgcctgcgg
     2221 tggcctggat gcccccacgg gggcctcgat ccggccgggc tgccctcgtg gtggccgagc
     2281 gtggcatcgc gtccctgctc ggtcaagggg gggatcctcg ccctctgctg cgcggtctac
     2341 tcgatgcggg cgcgctcgtg ctggccatcg acgcgttcgg cacgggggcc agtattgccg
     2401 cgaatccggc gagccagcgc gagggagtga agttcttcac gacgttcaac cgcaccgaca
     2461 cggcagagcg cgtgcaagat gtccttaccg ccatcgccta tctgcagtcg cgcgtcgacg
     2521 tggatcgggt ggatctggtg ggactcgaga aggccggcct gtggtgcttg ctggcgcggg
     2581 cattcgcgcc gtccgtgagt cgtgcggctc tcgacctcga tgggttcccg ggaaccgatg
     2641 aggcgtatct gtcggacctc tacgtgcccc tactcctccg ggccggcggc gtgcagaccg
     2701 ccggcgcgtt ggccgcaccg ggcgcgctgt tgctccacca tgtgagcccc gacctcgacc
     2761 tgtcgatgac ccgcgccgcg tacgcggccg cggatgccgg cgcgaacctg agagcggagc
     2821 gcgagaggct ctccgtcagt gaggtgctcc ggtggctgaa ccgctaggcc ggttcaccgc
     2881 cccgggcccc gtgatcgcag gggccaggtg aggcaatccc aggcgatccg gctgagcagc
     2941 cggggcgtga gctgttctcg gaacacctgg cggtacgagg tgcgaccgga aacgcagtca
     3001 tagagcgctc tctggagctc gggtgagtgc cgcgccaggg caatcacggc ctcgagccgg
     3061 aagggccccg agagcagggc tacgagcgcc cgcagcaggc ggccgtaggc gacgtcgctg
     3121 gcgagatcgc cgcatgccgc cacgtacgag gccccggcct ggctgcacgg cccgaactcg
     3181 agcagggcgc gtccggcggc ccggccggtg agcaacgccg cgcggatgcc cccgcctttg
     3241 aagggacgca ccatgccggc ggcatcgccg acgatgatgc agcgctcgct gaagatccca
     3301 cgcgcccggc cggcggggaa cgccccgcgg aagcagtcgc cgtgcgaggc agccgagggc
     3361 agcagctccc gaacccgcgg gcttgcgatg aacgctcgca tgtccgcctc ggtgacacgg
     3421 cggccggcca cgatgatcga cacgtggttc cccttg
//
LOCUS       DAOSPL010000332         3456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_27375_length_3456_cov_2.845928, whole genome shotgun sequence.
ACCESSION   DAOSPL010000332 DAOSPL010000000
VERSION     DAOSPL010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27375_length_3456_cov_2.845928"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="PLQ54_07345"
     CDS             complement(<1..1060)
                     /locus_tag="PLQ54_07345"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOS93112.1"
                     /translation="MTNREIADVLDQIGDMLELQGENVFKVRSYRRAAETIGRCSFAI
                     TGATASEQLRELPGIGESLSQLILEVAATGTCPLREQLLAVIPQGLLDVLRVPGVGPK
                     TAGALFHQLHVTDIKSLEEACRSGAVAALPRMGAKSQERILEGIALLQRSAGRILLSE
                     AAAIAARVIEQLPPPCEPVVAGSFRRGRETVGDIDILAVEVQGLDPMAAFTGMPEVEQ
                     VLLHGDTRSSVVVKGGVQVDLRVIPRPSMGAALQYFTGSQQHNIRVRERAGAMGLRLS
                     EYGLFDADDRQIAGASEAEVYGALGLDWIPPELREDAGEVARAAEHALPRLVELHDVR
                     GDLQMHSQWSDGAHTIRQM"
     gene            complement(1057..1815)
                     /gene="recO"
                     /locus_tag="PLQ54_07350"
     CDS             complement(1057..1815)
                     /gene="recO"
                     /locus_tag="PLQ54_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOS93113.1"
                     /translation="MATHSMRAITLVRRPVRDADQLVTFLVAERGVAVALARGSRKSG
                     SKLAPAVQPFAESDVLLASGRGGHLVLAQAQVVESHRGIRDNLRKLAHASYWVDLVAR
                     SVEEGQPAEGVFRLLHTSLRIASVVEDLWPATAYFQLHLLRLLGFEVSSARCASCGGE
                     IAAGPTAWSAAAGGCMCGACAASTPGSRLMSDRALDCIRHWAVTRPTAAYDESLDRQA
                     VAEAEGLVRAFVRYHIDAEPKTLSFLLQVEGLLA"
     gene            1992..2222
                     /locus_tag="PLQ54_07355"
     CDS             1992..2222
                     /locus_tag="PLQ54_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOS93114.1"
                     /translation="MCTMRGTVKWFNNAKGYGFLQPEGGGKDVFVHFSAIVADGYRSL
                     AEGDEVEFEIEDTEKGPQAINVSKTGSAADLA"
     gene            complement(2319..3110)
                     /locus_tag="PLQ54_07360"
     CDS             complement(2319..3110)
                     /locus_tag="PLQ54_07360"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOS93115.1"
                     /translation="MGTSGYSYDDWIGPFYPPDLPKSQFLTHYAERFQCVEINMTYYR
                     IPAPRQCAAWVRRTPSDFLFAVKANRLMTHERDNPDSLDAFPQFREAIQPIQEAGRLG
                     CVLLQFPQSFGLSDANSDHLVFVRDQLPDMPLVCEFRSAQWADDSTLGLLRQLDLGYC
                     CVDEPSIPGLMPRVAVATSPLGYLRFHGRNAAKWYDHEEAFERYDYLYQPHELAEWVP
                     KVGELAAATDHLFVFFNNHYNAQAVANADLFMEMLVEANLDVAGA"
     gene            complement(3135..>3456)
                     /locus_tag="PLQ54_07365"
     CDS             complement(3135..>3456)
                     /locus_tag="PLQ54_07365"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOS93116.1"
                     /translation="PVAVEATTGGQGQLQAQTRNISSRGAYFEVSRPIPELTRLEVTL
                     CVPSGSAESHQLHCGGVVVRCDARTTADGAQTHAIALFFDRIGPDEQALLDAFIAAQL
                     AAGA"
BASE COUNT          605 a         1074 c         1226 g          551 t
ORIGIN      
        1 ccatctgacg gatggtgtga gcgccatcgc tccactggga gtgcatctga aggtccccgc
       61 ggacgtcgtg gagttccacc agtcgcggca gggcatgctc ggcagcccgg gcgacttccc
      121 cggcgtcttc acggagctcc ggagggatcc agtccaggcc cagtgcccca tacacctcgg
      181 cctcagaggc accggcgatt tgccgatcat cggcatcgaa cagcccatac tcgctgagcc
      241 gaagacccat ggccccggca cgttcgcgga cgcggatgtt gtgttgctgt gagcccgtga
      301 aatactggag ggccgcgccc atgctgggcc gggggatgac gcgaagatcg acctggacgc
      361 cccccttcac gacgacgctg gagcgggtgt cgccgtgtag gagcacctgc tcgacctcgg
      421 gcatgccggt gaatgcagcc atggggtcca ggccttggac ttcgactgcc aggatgtcga
      481 tatcgccgac ggtctcacgc ccacggcgga agctgcccgc caccacgggc tcgcacgggg
      541 gggggagctg ctcgatgacg cgggcggcga tggcagcggc ttcgctgagc aggatgcgcc
      601 cggcggaccg ctggagcagc gcgatcccct cgaggatgcg ctcctggctc ttggcgccca
      661 tacgcggaag cgcggcgacg gcccccgacc ggcaggcctc ctcgagcgac ttgatgtcgg
      721 tgacgtggag ctggtggaag agggccccgg cagtcttggg gccgacgccg ggcactctca
      781 gcacatcgag gaggccctgc gggatgacgg caaggagctg ctcgcgcagt gggcacgtgc
      841 cggtggccgc tacctcaagg atgagctggg acagcgactc gccgatgccg gggagttcgc
      901 gaagctgctc ggacgcggtg gcgccggtga tggcgaagct gcaacggccg atggtctcgg
      961 ccgcgcggcg gtaggagcgg actttgaaga cgttctcgcc ctggagctcg agcatatcgc
     1021 cgatctggtc caggacatcc gcgatctcgc ggttggtcat gcgagcagcc cctcaacttg
     1081 aaggaggaag gagagtgtct tgggttcggc gtcgatatga taccgcacga aggcccgcac
     1141 gaggccctcg gcctcggcca ccgcctggcg gtcgagcgac tcatcgtagg ctgcggtggg
     1201 ccgggtgacg gcccagtgcc gaatgcagtc cagcgcccgg tcggacatga gccgagatcc
     1261 gggcgtcgag gcggcacagg cgccgcacat gcaccctccg gcggcggcgg accatgccgt
     1321 ggggcccgcc gcgatctccc cgccgcaact cgcgcagcgt gccgacgaca cctcgaatcc
     1381 gaggaggcgc agcagatgca gctggaaata ggcggtcgcg ggccacaggt cctcgaccac
     1441 cgaggcgatg cgaaggcttg tgtgcaggag gcggaacacg ccctccgccg gctggccttc
     1501 ctcgacactc cgcgcgacga ggtcgaccca gtaggacgcg tgcgccagct tccgcaggtt
     1561 gtcccggatt ccccgatggg attccaccac ctgagcttgt gcgagcacta gatggccacc
     1621 gcgcccggag gcgagcagga cgtcggactc ggcgaaaggc tggacggcgg gcgcaagctt
     1681 actccctgac tttcggctgc cacgggccaa tgcgaccgcc acaccgcgct ctgcgacgag
     1741 gaaggtgacg agctgatcgg cgtcgcgaac gggccgccgc acgagcgtga tggcgcgcat
     1801 gctgtgggta gccaccgctg tcctccatcc cggactggtg tgggaactcg ccggcgagcg
     1861 cccgtcgacg gcgtacgaac gccggacctc ttggtctcga cctttctggt tacggtagaa
     1921 gattgacacc agtaggggga gatgctaagc tccgtactgg agttttggac cagattgaac
     1981 ccaaggtagg tatgtgcact atgcgcggta cggtgaagtg gttcaacaac gccaaaggct
     2041 atggctttct gcagccggaa ggcggcggga aggacgtgtt cgtccacttc tccgccatcg
     2101 tggccgacgg ctaccggtcg ctggcagagg gcgatgaggt ggagttcgag atcgaggaca
     2161 ccgaaaaggg cccccaagcc atcaacgtgt ccaagacggg ctctgcggcg gatctcgcct
     2221 agccgtcggg cttccggacc acgggccacc cggctcgcgc cgaccgccga cagggcgctg
     2281 tggcgcgggg aggctgcgct tacggcccac atcccacatc aagctcccgc gacatcgagg
     2341 ttcgcctcca ccagcatctc catgaacagg tcggcgttgg ccacggcctg ggcgttgtag
     2401 tggttgttga agaacacgaa tagatgatcg gtagccgcgg cgagctcccc gactttcggt
     2461 acccactcgg caagctcgtg cggttggtag aggtagtcat accgttcgaa cgcctcctca
     2521 tgatcgtacc acttggctgc gttccggccg tggaatcgca ggtagcccag cggcgacgtg
     2581 gccaccgcaa cccggggcat gagaccggga atggatggtt catccacgca gcagtagccc
     2641 agatcgagct ggcgcagcag gcccagggtg ctgtcgtcgg cccactgagc gctgcggaac
     2701 tcgcagacga gtggcatatc ggggagctgg tcgcgcacga aaaccaggtg gtcgctgttg
     2761 gcgtccgata gcccaaagga ctgagggaac tggagaagga cgcacccgag gcggcccgcc
     2821 tcctggatgg gctggatggc ctcccgaaac tgcgggaagg catcgagact gtccgggttg
     2881 tcgcgctcat gggtcatgag ccggtttgcc ttgactgcaa agaggaagtc agacggcgta
     2941 cggcggaccc atgcggcgca ctggcgtggc gcggggattc gatagtaggt catgttgatc
     3001 tcgacgcact ggaagcgctc cgcgtagtga gttaagaatt ggctcttggg aaggtcaggc
     3061 gggtagaagg ggccgatcca gtcgtcgtag ctgtacccgg aagtgcccac atagaccaag
     3121 ggatcccccc ctgttcaggc tccggcggcg agctgcgcgg cgatgaaggc atcgagcagt
     3181 gcctgctcgt cgggcccgat gcgatcgaag aacagagcga tggcgtgggt ctgggcaccg
     3241 tcggcggtgg tccgggcatc acatcggact acgactccgc cgcagtggag ctggtgggat
     3301 tcggctgacc cgctgggcac gcagagcgtc acctcgaggc gggtgagctc ggggatgggg
     3361 cgagacacct cgaagtaggc gccacggctg ctgatgttgc gtgtctgagc ctgaagctgg
     3421 ccctgaccac cggtggtggc ctcgacggcg acaggc
//
LOCUS       DAOSPL010000333         3448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_27471_length_3448_cov_3.605659, whole genome shotgun sequence.
ACCESSION   DAOSPL010000333 DAOSPL010000000
VERSION     DAOSPL010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27471_length_3448_cov_3.605659"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..3326
                     /locus_tag="PLQ54_07370"
     CDS             240..3326
                     /locus_tag="PLQ54_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93117.1"
                     /translation="MNSWAVRVSVCLGVVGLSMPIHADSVTLKRALATAAVVGEERMT
                     NGGFETEEAGLAVGWARWSLGYTLEPDAARTGARGIRVISEDVNDQHGATYTARLGQP
                     RPFPIVVTGWSRAREVSGTPDNGYAIYLDITYIDGTPCWGVISSFATGTHDWEQRRVF
                     FVPEKPIRDVAVHALFRNHTGSADFDDFSLREMAGIEGGAFDSVPLLPANAPLDRAAE
                     GQVIPLASLAGLCAWDQATGALADTQGVLRGGLFVRDVAAGGDFVQLRGAMLRDGGAL
                     TMEASDEALGLRMTLRSEPLGGSTMVRGEVTDTTGRDRALSIYFSLPIDAAGWTWSDD
                     VRSDRAIEDGQTYLNGVLYGVGSTGRASRYPLAAIAGPETGVALAVPLDCPRVCRLAY
                     DAASRELYAAFDMALVPDAVAMPSRADFRLVVYRFDPAWRFRAALKGYYELFPDHFRR
                     VARHEGLWMPFTDIATVQSPEDFGFAYHEGDNNVAWDDAHDVLSFVYTEPMTNWMPMP
                     KETPRTYDAAVALMQERAEGGDSLAQATRTSAAFAADGRYDVAVLTAPWCDGAVFTLN
                     ADPGLRSSADQPSAAGVKLDAVQHAFATTASGVQGWQGRGDVVFADGAVKCRAPDPGG
                     ECMVIQSVTLNQAEPNALIARASSKAEGVTGTQDVDYSLYVDLTYADGDHLWSQAAPF
                     DVGTHDWQTREVRIEPAKPVQSATVHLLLRREHAGTAWFDDVFLGEADSMANRVVNGG
                     FDEGQPVAAVLDGTYIDSFEGWGTRPNYRREHFATVRVPLTFDTSSLQPVILTYFSTY
                     EFAAELRKRMSAQGKLVMANATPWTFPWAGHLLDVLGTETNWAPEGKYQPESDAVFNY
                     RRAVCYQKPYLLLQNTVYDTFTPEMIERYFKRSIFYACFPGFFSHNAADAPYWQNPVL
                     YNRDRPLFLKYIPLCKQISAAGWEPITHARTHDESVWIERYGYADQGNLHFTVFNAAT
                     AERQVTVTLDLEALGLAEAPATVRELVRGEDLQMTPAGEVRLSLGSEDLAILRPGG"
BASE COUNT          583 a         1203 c         1103 g          559 t
ORIGIN      
        1 cgcccctccg caaccaggcg gatgggccac gggagccctc agttaccacc aatgccttcg
       61 ggcggagccc caacccgggc ggcaaccagt agcccggtac cgaagccccg ggcaacatgc
      121 ttcgccgctc ccggcgctgc tactcgccac cgaggccccg gcgcgtccaa gggcgaaccc
      181 gagtcacgac gcccaggcga gccgcctgtg ccgccagtcc aagcggaggg ggactcacca
      241 tgaactcatg ggctgttcgg gtatctgtgt gcctcggagt cgtcggcctt tcgatgccga
      301 ttcacgccga ctccgtaacc ctcaagcgtg ccctcgccac ggcggctgtt gtcggcgagg
      361 agcgcatgac gaatggcgga ttcgagaccg aggaagccgg ccttgccgta ggctgggccc
      421 ggtggtctct tggctacacg ctcgaaccgg acgccgcccg aactggcgcg cgcggcatcc
      481 gtgtgatttc cgaggacgtc aacgaccagc acggagctac ctacacggcg cggctcggcc
      541 agccgaggcc gttccccatc gtcgtcacgg gctggagccg cgctcgcgag gtctccggga
      601 caccagacaa tggctatgcg atctatctgg acataaccta tatcgacggc accccgtgct
      661 ggggagtcat cagcagcttc gcgaccggca cccatgactg ggagcagcgg cgcgtcttct
      721 tcgtccccga gaagcccatc cgcgacgtcg cggtccatgc gctgttccga aaccacacgg
      781 gctcggctga cttcgatgac ttctcgctcc gcgagatggc cggcatcgag ggcggcgcat
      841 ttgattcagt gccgctacta cccgcaaacg ctcctctgga tcgcgccgct gaggggcagg
      901 tcatcccgct cgccagcctg gcgggcctct gcgcgtggga ccaggccacc ggcgcgttgg
      961 ccgatacgca gggcgtgctg cgcggaggat tgttcgtccg cgacgttgcc gccggcgggg
     1021 acttcgtcca gctccgtggc gccatgctgc gggacggcgg ggcgctgacc atggaggcct
     1081 ccgacgaagc cctcggcctc cgcatgaccc tccgctccga gcccctgggc ggcagcacca
     1141 tggtccgagg cgaagtcacc gacaccaccg ggcgagaccg agccctctcg atctacttct
     1201 cgctgcccat cgacgcagcc gggtggacct ggtccgatga cgtgcgcagc gatcgagcca
     1261 tcgaggacgg ccagacctac ctcaatggcg tgctctacgg cgtcggcagc acgggcaggg
     1321 cgtcccggta ccctctggcc gcgattgcgg gtcccgagac gggagtagcc ctcgccgtgc
     1381 ccctcgactg cccgcgcgtc tgccggctgg cctacgatgc cgccagccgt gagctctacg
     1441 ccgcctttga catggctctg gtgcccgatg ccgtggccat gccctcccgg gcggacttcc
     1501 gcctggtggt ctaccgcttc gacccggcat ggcgtttccg cgccgccctc aaaggctact
     1561 acgagctgtt ccccgatcac ttccggcgtg tcgcccggca cgaaggcctg tggatgccct
     1621 tcaccgacat cgcgaccgtg cagagcccag aggacttcgg cttcgcttac cacgaaggtg
     1681 acaacaacgt ggcgtgggac gacgctcacg atgtgctctc gttcgtctac accgaaccca
     1741 tgacgaactg gatgccgatg cccaaggaga ccccacggac ctatgacgcc gcagtggcgc
     1801 tcatgcagga gcgcgctgag ggaggagaca gcctggccca ggccacgcgc acgtcggccg
     1861 ccttcgctgc cgacggacgg tatgacgtgg ccgtgctcac cgccccctgg tgcgatggcg
     1921 ccgtgttcac gctcaacgca gacccgggac tgcggtcatc ggccgatcag cccagtgccg
     1981 caggcgtgaa gctcgatgcc gtccagcacg cgttcgcgac gaccgccagc ggtgtgcaag
     2041 gctggcaggg ccgcggggac gtagtcttcg ccgatggagc cgtcaaatgc cgtgcgccgg
     2101 accctggtgg cgagtgcatg gtcatccagt cggtcacgct gaaccaggcc gagcccaacg
     2161 ccctgatcgc ccgagcctcg agcaaggccg agggtgtcac gggcacccag gatgtcgact
     2221 actcactcta cgtcgacctg acgtacgcgg atggcgatca cctgtggagt caggcagctc
     2281 ccttcgacgt gggcacccac gactggcaga cccgcgaggt gcgcatcgaa cccgcgaagc
     2341 cggtccagtc cgcgacggtg cacttgctgc tgcgccgcga gcatgcgggc acggcctggt
     2401 tcgatgacgt gttcctcggc gaggccgact ccatggcgaa tcgggtcgtg aacggcggct
     2461 ttgacgaagg gcagccggtc gccgcggtgc tggatgggac ctacattgac tcattcgagg
     2521 gctggggcac gcgccccaac taccgccgag agcacttcgc cacggtccgt gtgccgctga
     2581 cattcgacac gagtagcctg cagcccgtga ttctgaccta cttctcgacc tatgagttcg
     2641 ctgccgagct ccgcaagcgc atgagcgctc agggcaagct ggtcatggcc aacgccacgc
     2701 cgtggacttt cccctgggcc ggccacctgc tcgatgtgtt ggggaccgag accaactggg
     2761 cgccggaagg caagtaccag cccgaatccg acgccgtctt caactaccgc cgggccgtgt
     2821 gctatcagaa gccctacctg ctgctgcaga acaccgtcta cgacaccttc acaccggaga
     2881 tgatcgagcg gtacttcaag cggtcgatct tctacgcatg tttcccgggt ttcttcagcc
     2941 acaacgccgc cgatgcgccc tactggcaga accccgtgct ctacaaccgc gaccggccgc
     3001 tgttcctcaa gtacatcccc ctgtgcaagc agatcagcgc ggcgggttgg gagccgatca
     3061 cccatgcacg gacccatgat gagtcggtgt ggatcgagcg gtacggctat gccgaccagg
     3121 gtaacctaca cttcacggtg ttcaacgccg ccaccgccga acgacaggtc actgtgacac
     3181 tcgacctcga ggctctgggg ttggccgagg ccccagccac cgtgcgtgag ctggtgcgcg
     3241 gcgaggacct gcagatgacg cccgctggcg aggtgcggct atcactcggc tccgaggacc
     3301 tggcgatcct gaggcccggg ggatagcggg cggcagaggc ggaaaccggt gacagtcacc
     3361 catttctagc attcaagaaa tacgtgactg tcgcgaatgg cacgcacttg gggtcggcac
     3421 ccacgcacac ccaaccgtag gcgaggct
//
LOCUS       DAOSPL010000334         3446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_27497_length_3446_cov_3.073725, whole genome shotgun sequence.
ACCESSION   DAOSPL010000334 DAOSPL010000000
VERSION     DAOSPL010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27497_length_3446_cov_3.073725"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="PLQ54_07375"
     CDS             <1..891
                     /locus_tag="PLQ54_07375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS93118.1"
                     /translation="HDRHAHLERHRVAQAIVGVGCGALEALVNALVADIHWRSPAGPL
                     NIVNGMYSVGLVAAALVTGEMVHAGYAWRATPWPWVFPALAVAMLFATRGYPVAQSDG
                     RGRAGMAFLRRPLFWVLMLAMVIGGGCEAGLTIWCTNFTEAELGATPRMGALITAFFG
                     AFMAAGRFGGSAAVRRVAAPLLVAGSSVGCAAGTAGLSFVQDTVAAWCLFALGGLLVA
                     CFWPTLLAIAVERVQAGSTAMMALLGAAGITGCTVFPWAIGALGDRVGLRGGLAVMPV
                     SLLVLASLCGAIWWRGRRGR"
     gene            complement(888..>3446)
                     /locus_tag="PLQ54_07380"
     CDS             complement(888..>3446)
                     /locus_tag="PLQ54_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93119.1"
                     /translation="AQEHGIHAVQISGWQQGGHDNGYPDYTPDRRLGTWDELAAGIRY
                     CHQLGLKVYFFANYQPMMVESDWYQNDLKRYREMTPDGGYTWMAGWGMGTLWARMGHP
                     KLMTWADLGFPEYRKIIVDQFARLAAIGADGVHVDKMFPTAVDYNPDIPLPPDISTWE
                     GAILLSREIMAACRARNPDWAMSFECNWDRMLEFGRATWWVGNQLITRQVFPENAETT
                     SITMAYDTLGINNLVRDGHIVMVTPQSFSRGLDWPPAAGLADYIKEVKRLRDGLADTV
                     FYGQSLGATGLRPDQHMPDGVQYSVFGNRTTGRHVCALTNARMEDRTLTFRGFEGSSS
                     WLVRIVTPFERPSQLVLPAEVRVPAERIVFVEELPTKGPGPIAASGAPKRVAPPAQSS
                     QSPANGGFETGDFTGWEADPNWVVVDNSCGYYSGWAGKWWAWSGGKGEPATGVLRSKP
                     FVLDQGGVALRVSGWASVRGTGQPRRWNYVTLNAEDGRELDRVYAPDTTAFVPAWLDG
                     SGHRGERVTIVAVDDADQPTFSMLCIDDVHTADPPGDFAQSVAPLPAFDPMRSIRLED
                     ERVLIEASRANGSITRIRDKQGGLELIREPRLAGSFRFSLVIPGKEPWQTMEANWVYG
                     HDQRLTSASADGSRLTLHWEGPLRNYLGEEFGVSATMTVRLTGDGARFDLAISNHTAL
                     PVGETYFPVLGGIQGLGTTRGQLKATRMVRPMPDGGTASDEIFRIFANQSPFGDQGPE
                     QFYACPAPQPEPWLAFVAPKANRSALIGAVDAAKRDLVFRLELVPSSSGTPRDDGNWP
                     RPEELNGQPVGVEMSLVDTSMGRAGDDHEAAPAFLQFCEGSDTGLRPAYESWQD"
BASE COUNT          484 a         1124 c         1234 g          604 t
ORIGIN      
        1 catgatcgac acgcgcatct tgaacgacac cgggtcgccc aggccatcgt cggggtgggc
       61 tgtggcgcgc tcgaggccct ggttaacgcc ctggtggccg acatccactg gcgaagtccc
      121 gccgggccgc tgaacatcgt caacggcatg tactcggtcg gcctggttgc cgccgccttg
      181 gtaaccggcg agatggtgca tgccgggtat gcctggcgcg ccacgccctg gccgtgggtg
      241 ttccccgcgt tggccgttgc catgctgttc gccactcgcg gttatcccgt tgcccagtcg
      301 gacggccggg gtagagccgg tatggcattc cttcgccggc cgctcttctg ggtcctcatg
      361 ctagcgatgg tcataggggg gggctgtgag gccggtctga ccatctggtg taccaacttc
      421 acagaggcgg agctgggcgc cacgccacgg atgggcgcgc tgatcaccgc cttcttcggc
      481 gcattcatgg ctgcgggccg cttcgggggc agcgcggcgg tgaggcgcgt ggcggcccca
      541 ctcctcgtcg ctggctcctc cgtcgggtgc gcggctggca cggccggcct cagtttcgtt
      601 caggacacgg tggccgcctg gtgcctgttc gccctgggcg gcctgctcgt cgcctgcttc
      661 tggccaacgc tcctcgccat cgccgtcgag cgcgtgcagg ccggttcgac ggccatgatg
      721 gctctgctcg gcgctgccgg gatcaccggg tgcaccgtct tcccgtgggc catcggcgct
      781 ctgggtgatc gagtgggcct ccggggtgga ctcgccgtca tgcccgtgtc gctgctggtt
      841 ctggcctcgc tgtgcggcgc gatctggtgg cgagggcgcc ggggacgcta gtcctgccag
      901 gactcgtagg ccggccgaag tcctgtgtcg cttccctcgc agaactgcag gaacgccggt
      961 gcggcctcat ggtcgtcgcc tgcccggccc atggaagtgt cgacaaggga catctcgaca
     1021 ccaaccggtt gtccattcag ctcttctggc cggggccagt tgccatcgtc gcgcggggtg
     1081 ccggagctgg acggcacgag ctcgaggcgg aacaccaggt cgcgcttggc cgcgtcgacg
     1141 gcgccgatga gggcggagcg gttggccttg ggagcgacga acgccagcca gggctctggc
     1201 tgcggtgcgg ggcatgcgta gaactgctcc ggcccctgat ccccgaaggg cgactggttg
     1261 gcgaagatgc ggaagatctc gtcgctggcc gtgcccccgt cgggcatggg gcgaaccatc
     1321 cgcgtggcct tgagctgccc tcgggtggtc cccaggccct ggatgccccc gagcacgggg
     1381 aagtaggtct cgcccaccgg cagtgccgtg tggttgctga tggccaggtc gaatcgcgcg
     1441 ccgtccccgg tgagcctgac ggtcattgtc gctgagacac caaactcctc gccgaggtag
     1501 ttccgcagcg gcccctccca gtgcagcgtc agccggctgc cgtcggcgct ggccgaggtg
     1561 aggcgctgat catggccgta gacccagttg gcctccatgg tctgccaggg ctccttgccc
     1621 gggatcacga gggagaagcg gaagcttcca gcgagacgcg gttcgcggat gagttcgagt
     1681 ccgccctgct tgtcgcggat gcgcgtgatg gagccgttgg cgcggctggc ctcaatgagc
     1741 acacgctcat cctcgagccg gatcgacctc atcgggtcga acgctggcag cggggcaacg
     1801 gactgggcga agtcgccggg cggatcggcc gtgtggacat cgtcgatgca gagcatggag
     1861 aaggtcggct ggtcggcgtc gtccacggcc acaatcgtga cgcgttcgcc cctgtggccg
     1921 gagccgtcga gccaggcggg cacgaacgca gtggtgtcgg gcgcgtagac acgatcgagc
     1981 tcgcggccat cctcggcgtt gagggtcacg tagttccacc ttcgcggctg gcccgtgccg
     2041 cggaccgatg cccagcccga gacgcgcagc gccacgccgc cttggtcgag cacgaagggc
     2101 ttcgagcgga gcacgcccgt ggctggctcg cctttgccgc cgctccacgc ccaccacttc
     2161 ccggcccagc cggagtagta gccgcaggag ttgtcgacga cgacccagtt ggggtcggcc
     2221 tcccagccgg tgaagtcgcc cgtctcgaag ccgccgttgg ccggcgactg cgaagactgg
     2281 gccggaggcg ccaccctctt gggcgcaccg ctggcagcga tagggccagg ccccttcgtg
     2341 gggagctcct cgacgaacac gatccgctcg gcgggaaccc gtacctcggc cggtaggacg
     2401 agttggctcg gcctctcgaa tggcgtcacg atgcggacga gccagctcga ggagccctcg
     2461 aagccgcgga acgtcagtgt gcggtcctcc atccgcgcgt tggtcagcgc acacacgtgc
     2521 cgaccggtgg tgcggttccc gaagacggag tactggacgc cgtcgggcat gtgctgatcg
     2581 ggcctcaggc cggtggcgcc aagtgattgg ccgtagaaga ccgtgtcggc caggccgtcg
     2641 cgaagccgct tgacctcctt gatgtagtcg gcgaggccag cagcgggggg ccagtccagt
     2701 ccacgagaga agctctgggg cgtgaccatc acaatgtggc catcgcgcac caggttgttg
     2761 atgccgaggg tgtcgtaggc catggtgatg ctcgtggtct cagcgttctc ggggaacacc
     2821 tgccgggtga tgagctggtt gccgacccac cacgttgccc gcccgaactc gagcatgcgg
     2881 tcccagttgc actcgaacga catggcccag tcgggattgc gcgcgcggca ggctgccatg
     2941 atctcgcgag agagtaggat ggcgccctcc catgtggaga tgtccggtgg cagcgggatg
     3001 tccggattgt agtcgaccgc ggtggggaac atcttgtcga cgtggacgcc atcggccccg
     3061 atggccgcca gccgcgcgaa ctggtccacg atgatcttgc ggtactcggg gaagccgaga
     3121 tcggcccagg tcatcagctt cggatggccc atccgggccc agagggtgcc catcccccac
     3181 cccgccatcc aggtgtagcc gccgtcgggc gtcatctcgc ggtagcgttt caggtcgttc
     3241 tggtaccagt cggactcgac catcatgggc tggtagttcg cgaagaagta gaccttgagg
     3301 cccaactggt ggcagtagcg gatgccggcg gcgagctcgt cccaagtgcc gaggcggcgg
     3361 tcgggggtgt aatcgggata gccgttgtcg tgcccgccct gctgccagcc gctgatctgc
     3421 acggcgtgga tgccatgctc ctgggc
//
LOCUS       DAOSPL010000335         3443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_27522_length_3443_cov_3.414699, whole genome shotgun sequence.
ACCESSION   DAOSPL010000335 DAOSPL010000000
VERSION     DAOSPL010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27522_length_3443_cov_3.414699"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3443
                     /locus_tag="PLQ54_07385"
     CDS             <1..>3443
                     /locus_tag="PLQ54_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93120.1"
                     /translation="RIVAVAAAICGVASVVYVRGRAVEWDLEDLRPFAEQEASAALGR
                     PVRMGRITGNVFRGITLHGLSIGRVAADGGAGDLLTAQRVVIRYSLWAVLRGRVSFQR
                     SIERIEVHRPQGEIRKLKDGSWQLARYVRQPKKKRPRTADRFAGIVQVYQGDLRFCDE
                     STPPGPGGRGLDYRGTGIDAIVDCHEVGLTQGTATMAVVDVADRATLTARYLSQGHVI
                     DILVDADGLRGDALFNWVVADDDPSFRFLAGTIPHVRAGFTWSDRASLEYFARGEVDG
                     MSFELPGVGAQPFAVEGDLVATSDHVASDRLWLTYGHSRAFVSGSVLGLNSQKEPKGP
                     ARIDLEISSKSMDFGEILQERPLPRETFERFVTADTGSADLRVVGSVEEVVVDGRIRL
                     GALYAKTTNGWEVSAPPMDIEGQLLCKGEYLADLTIPPSRMSVRVPEGLPYMGPRHPG
                     EAPPSREVRASGLMAARVTGPLSSPRIVGDLRLASASALGIGLTQLRGRVEAVQAPVP
                     PGSEVPPASESQSPLPATRWLVRLSDLESQVLEGSVRGAAAVEVMDGEVTWALTTRLE
                     DVDLAQLNRSVEVTNLPLEGRAFGTLVVSATPGDPMPDVALKARSPAVLVDRYDLGQV
                     VATAQLLDNGRVEVTQLSSSGQFGHFEASGTVPLFGDERTLDLAFESGTIDLAEFSRN
                     VTALSLDGEGLVRGSVQGTLDSPRVVGAAQALNGCVSWARAADGALIPASLSRPGQPS
                     VVYFDSAQARFEYHDRVISLADAELRRGPGRVRVLPVWVPQGMALAPFVGFEDDGTVR
                     FGMGLEAQDLPAGEVSQVLGIAEATQVDGLLSLERGMVAGQAQDYQFAGEVRLADATM
                     HGYGVEYASAAVLTQGDDLVIEDFSARVEETRVDGEGRVAQFRTAPEFVGVAWRADRI
                     SLARIDANVRRAHGLRGIASAMGGVDGPIQAPVITASAESDSVWLQGEEFTNVRLEMQ
                     YFVDPSDQEHVLWLGYPNPLVAHVGGGVLQAECEYELNARRISATGRMEGIATGPLSS
                     VTRAYLFEAARRGQETEAQRRVANLVRSLGGAVSGRFEVSGPVDDLEVRAQRIELADA
                     TYRGQPIPALAGSLRYQGQTVEVHELSLADPNGTASGSVWADLGPGGTILGNLTATGV
                     RL"
BASE COUNT          492 a         1090 c         1256 g          605 t
ORIGIN      
        1 cggatcgtgg ccgttgctgc cgcgatctgt ggtgtggcgt ccgtcgtgta tgtccgcgga
       61 cgtgcggtcg agtgggacct cgaggacctc cggcccttcg ccgagcaaga ggcgagtgcc
      121 gccctgggac ggccggtgcg gatgggccgg atcaccggta atgtgttccg cgggatcacg
      181 ctacatggcc tgagcatcgg ccgggtggct gcggatggcg gggcgggcga cttgctcacg
      241 gcgcagcggg tggtgatccg ctattcgctg tgggcggtgc ttcgtggtcg ggtcagcttc
      301 cagcgttcga tcgagcgcat cgaggttcat cggccgcaag gcgagatccg caagctcaag
      361 gatggctcgt ggcagctggc ccggtatgtc cgacagccca agaagaagag gcccaggacg
      421 gccgaccgtt tcgctggcat cgttcaggta taccagggcg acctgcgctt ctgtgacgag
      481 tccacaccac ccggtcccgg cggccgtggc ctggactacc gtggtacggg tatcgacgcc
      541 atcgtggact gccacgaagt cggtctgacg caaggcaccg ccaccatggc cgtcgtggat
      601 gtcgccgacc gggcgaccct gactgctcgg tacctctccc agggccatgt cattgacatc
      661 ctcgtggacg ccgatggtct gcggggtgat gccctgttca actgggtggt ggcggacgac
      721 gatccgagct tccggttcct ggccggcacc atcccccacg tccgggccgg ctttacgtgg
      781 tcagaccgcg cgtcgctcga gtacttcgcg cggggcgagg tcgacgggat gtccttcgag
      841 ctccccggtg tgggagcaca gcctttcgcc gttgagggcg atctggtggc gacttcggac
      901 cacgtcgcca gcgataggct gtggctcacc tacggccact cgcgggcctt cgtctcgggc
      961 agcgtgctcg gcctcaacag ccagaaggag cccaaggggc cggcccgtat cgacctcgag
     1021 atcagctcga agagcatgga cttcggggag atcctgcaag agcgccctct gcccagggag
     1081 accttcgaac gtttcgtgac ggccgacacg ggctcggccg atctgagggt cgttgggtcg
     1141 gtcgaggagg tggtcgtcga cggccggatc cgcctgggag ctttgtacgc caagaccacg
     1201 aacgggtggg aggtctcggc tccgcccatg gacatcgagg gtcagttgct ctgcaagggt
     1261 gagtacctgg ctgacctgac gattcccccc agccggatga gtgtccgggt gcccgaggga
     1321 ctgccctaca tggggccccg gcatcctggc gaggcgccgc cgagccggga ggttagggcc
     1381 agtggcctga tggcggcccg ggtgaccgga ccgctgtcat ccccgcgcat cgtgggcgat
     1441 ctgaggttgg catcggcctc agccctcggg attggcctga cccagttgcg gggccgagtc
     1501 gaggccgtgc aggctccggt ccctccgggt tccgaggtgc cacccgcgtc ggaaagccaa
     1561 tctccgcttc cggccacccg ctggctggtg cgcctgagcg acctcgagtc acaggtgctc
     1621 gaagggagcg ttcgcggggc ggcggccgtc gaggttatgg acggagaggt cacctgggcg
     1681 ctcacgacgc gactggagga tgtcgacctg gcccagctca accgttcggt cgaggtcacg
     1741 aatctgccgc tggaggggag ggccttcggc acactggtcg tctcggcgac cccgggcgat
     1801 ccgatgcccg acgtcgccct gaaggcccgg tcgccggccg tgcttgtcga ccgctacgac
     1861 ctgggccagg tggtggccac cgctcagctc ctggacaacg gccgagtcga ggtgacgcag
     1921 ctctcatcct ccgggcagtt tgggcacttc gaggcgagtg gtactgtgcc tttgttcggc
     1981 gatgaacgca ctttggacct tgccttcgag agcggcacca tcgacctggc cgagttcagc
     2041 aggaacgtca cggctctctc cttggatggg gaggggttgg ttcgtggatc ggtgcagggc
     2101 acactagact cgccgcgagt cgtgggtgcg gcccaagcgc tcaacgggtg cgtgtcgtgg
     2161 gctcgcgccg ccgacggcgc tttgatcccc gcttccctca gccggcccgg gcagccgagc
     2221 gtggtctatt tcgattcggc ccaggcccgc ttcgagtacc acgaccgggt catctcgctg
     2281 gccgacgccg agctgcgacg ggggccgggc cgggtccgtg ttctgcccgt atgggtgccc
     2341 caggggatgg cgctggcacc cttcgttggg ttcgaggatg acggcacagt gcgcttcggc
     2401 atgggcctcg aggcccagga tctgcccgcc ggcgaggtga gccaggtcct cggcattgcc
     2461 gaggccacgc aggtggacgg gctgctctca ctggagcgcg ggatggttgc tggtcaggca
     2521 caggactacc agtttgccgg cgaggttcgg ctggcggatg ccaccatgca tggttacggc
     2581 gtggagtacg cctcggccgc cgttctcaca cagggcgacg acctggtgat cgaggacttc
     2641 agtgcgcggg tcgaggagac ccgtgtcgat ggcgagggcc gcgtggcgca gttccggacc
     2701 gcgcccgagt tcgtcggagt ggcatggagg gccgatcgga tctcgcttgc gcgcatcgat
     2761 gccaacgtgc gccgcgccca cgggctgcgc ggcattgcgt cggccatggg aggcgttgac
     2821 ggccccattc aggccccggt catcaccgcg tcggctgaga gcgactcagt gtggctccag
     2881 ggcgaggagt tcaccaacgt ccggctcgag atgcagtact tcgtcgaccc gtccgatcag
     2941 gagcacgtcc tttggctggg gtacccgaac cctctggtgg cccatgtggg cggcggcgtg
     3001 ctgcaggccg agtgcgagta tgagctgaac gcccggcgga tctcggcgac ggggcggatg
     3061 gagggcatcg ccaccgggcc gctgtcatcg gtcacccgtg cctatctgtt cgaggcggct
     3121 cgacggggtc aggagaccga agcgcagcgc cgagtggcga acctggtgcg ttccttgggc
     3181 ggcgcggtct cgggccgctt cgaggtgtcc ggccccgtcg atgatctcga ggtccgcgcc
     3241 caacggatcg agctggctga cgcaacctac cggggccagc ccattccggc gctggcgggc
     3301 tcgttgcggt accagggaca gacggtggag gtccacgagc tatcgcttgc tgaccccaac
     3361 gggacggcca gcggatcggt ctgggccgat ctgggtcccg gagggaccat tctcgggaac
     3421 ctgaccgcca ccggggtacg act
//
LOCUS       DAOSPL010000336         3442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_27540_length_3442_cov_2.986123, whole genome shotgun sequence.
ACCESSION   DAOSPL010000336 DAOSPL010000000
VERSION     DAOSPL010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27540_length_3442_cov_2.986123"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLQ54_07390"
     CDS             <1..474
                     /locus_tag="PLQ54_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93121.1"
                     /translation="TPLCIGLTAADGNPRDDLVATSTPEGVIHLLLEGCYLFILGDQK
                     EGERLCRAALVDPPSETSWVRFVSCLKEKQIIFYSYAQGSTNEGQYVNFDPTSFTVKI
                     TRTEKLGEEIKAFIASSGADNPRPFRSKVTPRGQLRIVEWSSIYVDVKPVPKATW"
     gene            521..2863
                     /locus_tag="PLQ54_07395"
     CDS             521..2863
                     /locus_tag="PLQ54_07395"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOS93122.1"
                     /translation="MSRQAAYVGLLAIAGGIVQAADLTLVAEGRPSATIVVREGAPEK
                     VTVAAEELRTYVERISGAQLPLATDGERPDGAVVLVGRSRLTDQMGVVIPGGLTDGRR
                     DEGYTIVCRGNRLVLAGNESGPYHGTEYAVYEFLHRLGVRWYMPGPNGEVVPHSETIR
                     FPEMDRSDTPDFAMRNWWLHIKPELAEHERRWKLRNKMNPDPLFATPGDSSARNIVRP
                     ELLETRPELFARNADGTPNPYLPNLTNPEAVTVAADIVKEYFRQHPEANSYGFAPDDG
                     LPRDYTPETQAINRGFTDLLGRPGVPGEVSTTEEWLAFVNAVTREVRREFPDRYIATN
                     GYANRNIPPQGIELDDHIIIMFAAIWSCTLHAYDNPHCWQKERQAQMLRRWAELCPNV
                     WVYGYNYQMLVSGLTPLPEFTKLRRDFPLMHRWGLLGFLDENRNVWAEAGIASRYLRA
                     RLEWDAEADVDAILDDFFRDWYGPAAAHMKAYYFALDEAITESPMHAHEDRALPDVYS
                     DRLLERLSDHISQAEDLATAEPFRSRLQADLLMFDHLVAYRAMNAAERDADFTRAARH
                     AQEMLDLRKRLFAIDPFYIWEDEDGYHTGVWYWKIGDRRDWYRSLAERMTGPTGELAA
                     TLPERASFRTDPHDEGLHAGWYAVADPPGEWPEVSVTQPFFTQGYLDDRGFPYVGYVW
                     YRFGLDLPANVAGRSVRLLIPTLTTEGWLWVNGQYVAHRPYLEAYTRPASLEADISAQ
                     VHPGARNVVAVRVSTSLCESQAAEGILGRPFVYTPLEPTE"
     gene            2907..>3442
                     /locus_tag="PLQ54_07400"
     CDS             2907..>3442
                     /locus_tag="PLQ54_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93123.1"
                     /translation="MIAFVRCLTLVPLILTGAVGAAGAPLTARIEPNGSMTIRDGRVV
                     LASVSAGFFEDEWQGRSLSGASAAGAELAPGERTAHFALPDDRGTIDLRVAFQTADGR
                     LVARYALKPSVELKVNSIHVSLDLPLRLWVGGTWAAGDRQGEFTPERGPTGVYSGRPQ
                     ALKLTAAGPVWDEIKIVPP"
BASE COUNT          591 a         1183 c         1118 g          550 t
ORIGIN      
        1 accccgctgt gcatcggcct gacggccgcg gacggcaacc cgcgtgacga cctcgtagcc
       61 acaagcacgc ccgagggcgt gatccacctg ctgctcgaag gctgctatct gttcattctg
      121 ggcgaccaga aggagggcga gagactctgc cgcgccgctc tcgtcgatcc gcccagcgag
      181 acctcgtggg tccgcttcgt cagctgcctc aaagagaagc agatcatctt ctacagctat
      241 gcccagggct caaccaacga ggggcagtat gtgaacttcg accccacgag cttcaccgtg
      301 aagatcaccc gcaccgagaa gctcggcgag gagatcaagg cgttcatcgc cagcagcggc
      361 gccgacaacc ccaggccgtt ccggtcgaag gtcacgccac ggggccagct gaggatcgtc
      421 gagtggtcgt cgatctatgt cgacgtgaaa cccgtcccga aggcgacgtg gtagcgcgag
      481 gccgctcgcc cggcggggca gcagtaggag gtcgccgcgc atgtcccgac aggctgccta
      541 cgtgggcttg ctggccatcg ccggcgggat cgtgcaggcc gcagatctca ccttggtggc
      601 ggaggggcgt ccctccgcca ccatcgtcgt acgtgagggc gctcccgaaa aggtgaccgt
      661 ggccgccgag gagctccgca cctacgtgga gcgcatctcc ggcgctcaac tgcctctcgc
      721 tacggacggg gagaggcccg acggtgccgt agtgctggtg gggcgcagca gattgaccga
      781 ccagatgggc gtcgtgattc ccggcggcct gaccgacggc cggcgcgacg aggggtatac
      841 catcgtctgc cggggcaacc ggctcgtgtt ggcgggcaac gagagcgggc cttaccacgg
      901 cacggagtac gccgtctacg agttcctaca ccgccttggc gtccgctggt acatgcccgg
      961 gccgaatggg gaagtcgttc cgcacagcga gaccatccga ttccctgaga tggaccggag
     1021 cgacacgccc gacttcgcca tgcggaactg gtggctgcac atcaagcccg agctggctga
     1081 gcacgagcgc cgatggaagc tccgcaacaa gatgaacccc gacccactct tcgccacgcc
     1141 aggcgactcg tcggcgcgga acatcgtccg ccccgagctg ctcgagacgc gcccggagct
     1201 gttcgcccga aatgccgatg gcacgccgaa cccgtatctg ccgaacctca cgaaccccga
     1261 ggcggtcacc gtggccgccg acattgtcaa ggagtacttc cgccagcacc ccgaggccaa
     1321 cagctacggg ttcgcccccg atgacggcct cccgcgcgac tacaccccgg agacgcaggc
     1381 catcaaccgc ggtttcaccg atctgttggg gcggcccgga gtgccgggcg aggtcagcac
     1441 caccgaggag tggctcgcct tcgtcaacgc ggtcacacgt gaggttcgga gggagttccc
     1501 ggacaggtac atcgccacca acggctacgc caaccggaac attccgcccc agggcatcga
     1561 gcttgacgac cacatcatca tcatgttcgc ggcgatctgg agctgcacgc tccacgccta
     1621 tgacaacccc cactgttggc aaaaggagcg ccaggcacag atgctgcggc gctgggccga
     1681 gctgtgcccg aacgtgtggg tgtacggcta caactaccag atgctcgtct cgggcctcac
     1741 tccgctccct gagttcacca agctgcggcg cgacttcccc ctgatgcacc ggtggggcct
     1801 gttgggtttc ctggatgaga atcgcaacgt gtgggccgag gcgggcatcg ccagccggta
     1861 ccttcgcgcg cgtctcgagt gggacgccga ggccgacgtc gatgccatcc tcgatgattt
     1921 cttccgcgac tggtacggcc ccgccgcggc gcacatgaag gcgtactact tcgccctcga
     1981 cgaggccatc accgagagcc ccatgcacgc ccacgaggac cgagcgctgc cggacgtcta
     2041 ctccgatcgg ctcctggagc gactgagtga ccacatctcg caggccgaag acctggccac
     2101 ggcggagccg ttccgcagcc gtctgcaggc cgacctgctc atgttcgacc atctcgtcgc
     2161 ctaccgcgcg atgaacgcgg cggagcgcga tgctgacttc accagggctg cgcggcatgc
     2221 ccaggagatg ctcgatctac ggaaacggct cttcgccatc gaccccttct acatctggga
     2281 ggacgaagac ggctaccaca ccggggtgtg gtactggaag atcggcgacc gacgcgattg
     2341 gtaccggtca ctcgcggagc ggatgaccgg ccccaccggc gagctcgcgg ccacgttgcc
     2401 cgagcgcgcg tcattccgaa cggacccgca cgatgagggg cttcacgcgg gctggtacgc
     2461 cgttgccgac ccgccgggcg agtggccgga ggtctcggtc acccagccgt tcttcaccca
     2521 gggctacctt gacgaccgcg gcttccccta tgtgggttac gtctggtacc gcttcggtct
     2581 cgatctgccc gcgaacgtgg ccggcaggtc tgtgcggctg ctgatcccga cactgactac
     2641 cgagggctgg ctctgggtca acggccagta tgtggcccat cgtccgtacc tggaggccta
     2701 cacacgtccg gcgagcctcg aggccgacat cagcgcccag gttcacccgg gcgcgcggaa
     2761 tgtggttgcg gtccgagtca gcaccagtct ctgtgaatcg caggcggccg aaggcatctt
     2821 gggccgcccg tttgtctaca caccgttgga gccaacggag tagtgatcct ccgcgcaacg
     2881 cacctgccga atgggggcat tgggtcatga tcgccttcgt acgttgcctc acactcgtgc
     2941 cgctgatcct gaccggggca gtcggcgcgg ccggcgcgcc attgacggct cgtatcgagc
     3001 ccaacgggtc catgaccatt cgcgacggcc gggttgtgct cgccagtgtg tccgccggct
     3061 tcttcgagga tgagtggcag ggccgaagcc tctcgggcgc gtcggcggca ggtgccgagc
     3121 tggcgccggg tgagcggacg gcacacttcg ccctgcccga tgaccgcggg acgatcgatc
     3181 tgcgcgtcgc attccagacc gccgatggca ggctcgtcgc ccgttacgcg ctgaagccca
     3241 gcgtcgaact gaaggtcaac agcatccacg tttcactcga tctaccactc cggctctggg
     3301 tcggcgggac ctgggccgcc ggggaccgcc agggcgagtt cacgcccgaa cgcgggccga
     3361 cgggtgtcta ctccggccgg ccgcaggcgc tgaagctcac cgcagccggc ccggtctggg
     3421 acgagatcaa gatcgtcccg cc
//
LOCUS       DAOSPL010000337         3435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_27598_length_3435_cov_3.327811, whole genome shotgun sequence.
ACCESSION   DAOSPL010000337 DAOSPL010000000
VERSION     DAOSPL010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27598_length_3435_cov_3.327811"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PLQ54_07405"
     CDS             <1..556
                     /locus_tag="PLQ54_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93124.1"
                     /translation="TATDPIEGGNTTTYTYPQDPINAADLSGDRYTIKAMKYFSEFGG
                     LSNNIGSVGEAAGFGGSGGLRANQANGAAARNSMARAANGKNVLIDMEVGIATSAGRR
                     VVDVLSSSFRNGAVRLRAFESKVGRTGLNKRTRAQIAKDRALLERGIRVTWVFSRSPV
                     TGKGGATLKLQDALTDVGINFISR"
     gene            774..1703
                     /locus_tag="PLQ54_07410"
     CDS             774..1703
                     /locus_tag="PLQ54_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93125.1"
                     /translation="MREPRWVMSITVEGSGAAGIRVAQKLSREVAKLTGAAVYDQQAG
                     RVLVDGKLRRPQRVDDDEGSLQVKFGWYSLVSQVKDLPPLPLHWLDLATSLLPEAAPV
                     RYGDLEPPRYRLDRDGTEPLARLWHSPDGWRPSVYADLARPALLASWGQQPVGAGDFM
                     MWERASLLVEPEAVRDPFWQAAMRRFFLAVAVERRCVYALSQPDHTSPYEPHARVLTP
                     GGRWYGLHPRPQWWCWYGPPYADVVADRLPEAAYERHGSGIFVVTGSGPFDVDGAGPD
                     PLADLRAAPSSRERRGHHDPNLLPAQVVPAGLM"
     gene            complement(2323..2529)
                     /locus_tag="PLQ54_07415"
     CDS             complement(2323..2529)
                     /locus_tag="PLQ54_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93126.1"
                     /translation="MRTPIVTTSPTPPTHNTPHHATFPQVTALPDDFAEALRAFRVTV
                     VWLDAPKIRTEHPSSTIRWNPLAE"
     gene            2562..3047
                     /locus_tag="PLQ54_07420"
     CDS             2562..3047
                     /locus_tag="PLQ54_07420"
                     /inference="COORDINATES: protein motif:HMM:NF025180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HOS93127.1"
                     /translation="MSSSPTAAASRISLVQVFTGVPDPRSRRGVRHALPVVLTVAAAA
                     VLAGARSLLAIGEWVAEADRDALSQLGIGVDVVLSTESTIRRTLAGLDADDLDARLGA
                     WMATRVGQVAGRRVIAVDGKNMRGAVADGVMPHLLAVLDHQHRVVLSSGSNSLRPERH
                     L"
BASE COUNT          560 a          980 c         1228 g          667 t
ORIGIN      
        1 caccgccacc gaccccatcg aaggcggcaa caccaccacc tacacctacc cccaagaccc
       61 catcaacgct gcggatctgt ctggcgacag gtatacgatc aaggcgatga aatacttcag
      121 cgagtttggg ggcctgtcca acaacatcgg cagcgtcgga gaagccgcgg gattcggtgg
      181 aagcggagga ttgagagcca atcaagccaa cggggcggcc gcgcgcaatt ctatggcgcg
      241 tgctgcgaat ggcaagaacg ttctcattga catggaagtc gggatagcga cttccgcagg
      301 gcggcgggtg gtggatgttc tttccagctc cttccgtaac ggtgcggtac gcctgcgggc
      361 gttcgagtcg aaggtcggtc gtaccgggct caataaaagg actcgagccc aaatagctaa
      421 ggaccgggct ttgcttgaaa gaggtattcg cgtgacgtgg gtgttctcca ggagtccagt
      481 gaccgggaag ggcggagcaa cgctgaagct ccaagatgcg ctaactgacg tcggcatcaa
      541 cttcatctca aggtagtacg gagcgagcca atatgtctta tgaccttcaa gtgtttgctc
      601 gttcggggtt gacgccgact gtgattgctg cggtggtggc tgatcttggg ttggtgtggc
      661 gcgcatcggt tgatacgtgg gatgccctcg acgctcgggg agttccggtg tgtggcgggg
      721 atgtcccggt gccgatggaa gttgaggatc tcccggagga gttgtccggg atcgtgcgtg
      781 agcctcggtg ggtcatgtcg atcaccgtcg agggcagcgg tgcggctggt atccgcgtcg
      841 cccagaagtt gtctcgcgaa gtggccaagc tgaccggggc agcggtgtac gaccagcagg
      901 cgggacgggt tctggtcgac ggcaagctgc gccgtcctca gcgggtggac gacgacgaag
      961 gctcactcca ggtcaagttc ggatggtatt cgctggtctc gcaggtcaag gatttacctc
     1021 cgttgccgct gcactggttg gacctggcga cctccctgct tccggaggcg gcgccggttc
     1081 gttacggcga cctcgagccg ccgcggtatc ggcttgaccg ggacgggacc gagccgctgg
     1141 cgaggctgtg gcacagtcct gacggctgga ggccgtcggt gtacgccgat ctggcacggc
     1201 cggcgctctt ggcgtcgtgg ggccaacagc ctgtgggggc gggggatttc atgatgtggg
     1261 aaagagcctc gcttcttgtc gagccggagg cggtgcgtga cccgttctgg caggctgcga
     1321 tgcgtcggtt ctttctggcg gtggctgtgg agcggcgctg tgtgtatgcg ttgagtcaac
     1381 cggaccatac gagtccctac gagccgcacg cgcgggtgct cacccccggg ggccggtggt
     1441 atgggttgca tccgcggccg cagtggtggt gttggtatgg cccgccgtat gccgatgtcg
     1501 tggccgatcg gttaccggag gcggcctatg agcgtcatgg gtcggggatc ttcgtggtca
     1561 ccgggtcggg gcccttcgac gtcgatggcg cagggccgga cccgctggcg gatctgcggg
     1621 cagcgccgag ttcccgcgag cgccgtggcc accatgaccc gaaccttctc ccagcccagg
     1681 tcgtccctgc cgggctcatg tgaccaccgg cccaggcacc tccatcactc ccgggttgct
     1741 tcttgtaggt agatctgtgg gctgggcgag cggaaggggc gaagatggtg accatcgcgg
     1801 aggaaggcaa ctcggggagt tcgtttccca gccgaggtgt cagggcgcgt gctccacggg
     1861 tgagcaagca ggcaggtttg taaggagcct cctcccgaag tggggcggcg acgtgatcga
     1921 cttcgtgggc ggcttgtgat gacaacaggg acctctggga acagcgaatc agctccatcc
     1981 aaaccgggcc tgaactttgg cacggttctg atcgcattgg gattagtggc ttgggtggga
     2041 tccatgctat ccgtaccgat gtcggccata ttcggcggtc gcgagaccta caactggagc
     2101 atattggtgg ctgttcactt gggcagttac cctattgtag ggctcggcgc gtggctagat
     2161 gcgagaatct caggctccac cattagacag agcgttcggc gcgcactcag cgcgatattc
     2221 gaaatgataa cgtttccata atagctcgag gagtagcaaa cgacggacat cctcgagttc
     2281 atacaagagc ccaaagtcgg agacccggtt tatcgcgtcg cactattcgg ccagggggtt
     2341 ccagcggatc gtcgagcttg ggtgttccgt acggatcttt ggggcatcga gccaaacgac
     2401 ggtgacccgg aaggcccgca gggcctcggc aaagtcgtcc ggtagggcgg tgacctgcgg
     2461 aaacgtggcg tggtgcggcg tgttgtgtgt ggggggcgtg gggctggtgg tgacgatggg
     2521 agttctcaca ccacccttgt cgccgggagc cccacgccgc catgtcatct tcccccaccg
     2581 ctgccgcttc tcgcatctcg ctggtccagg tgttcaccgg tgtgcccgac ccccgcagcc
     2641 gacgcggggt gcgccacgcg ctgccggtgg tgttgacggt ggccgctgcg gccgtgctgg
     2701 ccggcgcgag atcattgctg gcgatcggcg agtgggtcgc cgaggccgac cgggacgcat
     2761 tgtcccagtt gggaatcggc gtggatgtgg tgctgtcgac ggagtcgacg atccgacgga
     2821 ccttggccgg gttggatgcc gacgacctcg atgctcgcct cggggcgtgg atggccaccc
     2881 gagtggggca ggtcgctggc cggcgggtca tcgccgtgga cggcaagaac atgcgcggcg
     2941 cggtagccga cggggtgatg ccgcacctgc tggctgtcct ggaccaccag catcgggtcg
     3001 tgctgtccag cggcagcaat tcactgcgac cggaacggca cctgtaaccg gtgtccgtcg
     3061 cggccggggc gtgatggttc gtcctggtcg accttcgcgg cggaagcgat gcccagtgag
     3121 ggcattcaag gagatgagtg gggtgcctgc agccgtgctc gatacgtcca tcggagacga
     3181 acgtgggatc accgtgacag cggatacggg aaactgcccg gaggcggcca tgtagctgcc
     3241 cggtagcggt cacgagtgtt gcccgctggt ggtcatggga tctgcccgac acgagttcgt
     3301 ctgccttgcc gcgtcccgcg gttgaggccc ctcctgggct gcggttggac tcaacctcac
     3361 cacggctacc gggtacgagg ctcccggtgt cggctacctg cggcgcagca cccggaccct
     3421 gccgtctggg acgtc
//
LOCUS       DAOSPL010000338         3429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_27665_length_3429_cov_3.031713, whole genome shotgun sequence.
ACCESSION   DAOSPL010000338 DAOSPL010000000
VERSION     DAOSPL010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27665_length_3429_cov_3.031713"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="PLQ54_07425"
     CDS             <1..1087
                     /locus_tag="PLQ54_07425"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HOS93128.1"
                     /translation="AGYQWNTLDCAVGGSTSWLGSLYLAALAASERMAALQGDPQRAE
                     RYRAIRNRGAVTQDATLFNGEYYIQLPDPEPRNDYVDGCAIDQLLGEWWATQVGLPTA
                     YPDDHARRALSALIEHNFRPTLAGVTQSPRKFVADEDAGLQMIQWPKGDRPSPCILYG
                     DEIMTGFEYAAAATMVQKGMLREGLMVAKAVSDRYDGRLREGLTPGDTASWGYSGNPF
                     GDDECGKYYGRAMSVWSLLLACQGFSYDGPAHRIGFAPIWQPEDHSSFFTAAEGWGVY
                     RQRRMPGSLRAVLDVRFGRVAVRELTLALPEGVNCSGGRVAMGGSEVPATIAGGGRGV
                     QVRIGKDRLVTAGQELVVELAFEAPDG"
     gene            1153..1908
                     /locus_tag="PLQ54_07430"
     CDS             1153..1908
                     /locus_tag="PLQ54_07430"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOS93129.1"
                     /translation="MLLDGKVVIVTGGGRGIGANVSRVLARHGARVAVVYSTSKSKAQ
                     TVVTEIAKAGGTAKAFQADVRDVDSVETMVKAVKRQFKRIDGVVNNAIAGRQHAPFDQ
                     ATWTDYQDMFDFGCKAVVNLIRAVRPIMKAQGGGRIVNIVTELWNMGSAEWSTYLAGK
                     GAMVGLSRSLASELGPEGITINMVAPGWMADEKVDPSSDASIRFGQTLPVRFHGSADE
                     IGNGCVFFLSDLARYVTGAYLPVTGGRVTQMGA"
     gene            1936..3066
                     /locus_tag="PLQ54_07435"
     CDS             1936..3066
                     /locus_tag="PLQ54_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS93130.1"
                     /translation="MSRPIGMGVVGAGSIGIRGALQHLCLPDVQDRVRLAAVCDPVPG
                     RAAAAAAKYGVPHAYQSFEELLADDSVDAVTLGSPIGLHYEQGLAAVRAGKHVHFNKT
                     MTTTASEATKIINEAAAHRVRLVASPGQMLRPINQRIRRLVQDGALGRLAWAATGAGF
                     GTYHENEGCRQGDDVLSNINPAWYWRRPGGGPLFDMTVYGLHSLTGVLGPAQRVAGMS
                     GVAVREREFQGTRYACDADDNTLLLLDFGNQTYAFVYGALSGMLTEFGLASYFGTRGH
                     IAGRTMNGEPIDYPGRTEAEAAGGDNVLLPHVVGPHRELDEAHVFEDIMQLVDWAREG
                     IPSPATAEHARHVIEIFEAGYRAAETGETQTLTTTFELPDQG"
     gene            3128..>3429
                     /locus_tag="PLQ54_07440"
     CDS             3128..>3429
                     /locus_tag="PLQ54_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93131.1"
                     /translation="MRGRDCTMDTTVGSPVPKRAGRLIWFQAAGAVVTSMGAFLVLCL
                     CVIIPSMRASLIDGKREAIADHTSVAVAILAESYAKERAGKATREEAQRDALRVLRG"
BASE COUNT          589 a         1021 c         1265 g          554 t
ORIGIN      
        1 ggcggggtac cagtggaaca cgctcgactg cgcggtgggc gggagcacgt cgtggttggg
       61 gagcctgtat ctggctgcgc tggcagccag cgagcgcatg gcggctctgc agggcgatcc
      121 gcagcgcgcc gaacgctacc gagccatccg caaccgcggc gccgtgacgc aagatgccac
      181 gctgttcaac ggggagtact acatccagtt gccagatccc gagccgcgca acgactacgt
      241 cgacggatgc gccatcgacc agcttctggg cgagtggtgg gccacccagg tgggactgcc
      301 gaccgcctat cccgacgacc atgcgcgccg cgctctgtcg gcgctaatcg agcacaactt
      361 ccggccaacg cttgcgggcg tgactcagtc gcctcggaag ttcgtcgccg atgaggatgc
      421 gggcctgcag atgatccagt ggccgaaggg agacagaccc tcgccgtgca tcctctatgg
      481 ggacgagatc atgactggct tcgagtatgc cgccgcggcg acgatggtgc agaagggtat
      541 gctccgtgag gggctcatgg tggccaaggc ggtcagcgat cggtacgacg gacggcttcg
      601 ggaggggctg acaccgggcg acacggcatc gtggggatac agcgggaacc cgtttgggga
      661 cgatgagtgt ggcaagtact acggccgagc gatgagtgtc tggtcgctcc tgctcgcctg
      721 ccagggattc tcatacgacg gcccggcgca ccgcatcggc ttcgctccca tctggcagcc
      781 ggaggaccac tcatcgttct tcacggcagc ggaggggtgg ggcgtgtacc gtcagcgtcg
      841 catgcccggg tcgctccgcg cagtgctgga cgtgcgcttc ggccgggtgg ccgtccgcga
      901 gctgacacta gcgctgcccg agggcgtgaa ctgcagcgga ggccgggttg cgatgggcgg
      961 gagcgaggtt cccgcgacga ttgcgggcgg tggccgtggc gtgcaagtcc gtatcggcaa
     1021 ggaccgactg gtcaccgcag gtcaggagct ggtggtggaa ctggcgttcg aggcgccaga
     1081 cgggtagtcc gtcgtcaggc ggcgcttcgt ctggatgact ctgcatgact gcaaccctgg
     1141 gaggatcgag cgatgctgct ggacggtaag gtcgtcatcg tcacgggggg cgggcgcggg
     1201 atcggtgcga acgtgtcgcg ggtgctggcg cgccatgggg cgcgggtggc cgtggtgtac
     1261 tcgacgagca agagcaaggc gcagacggtg gtgacggaga tcgcgaaggc cggcggaacg
     1321 gcgaaagcgt tccaggcgga tgtgcgcgat gtggactccg tcgagaccat ggtgaaggca
     1381 gtgaagcgcc agttcaagcg catcgatggc gtggtcaaca atgccattgc gggccggcag
     1441 cacgcgccgt tcgaccaggc cacctggacc gactaccagg acatgttcga ctttggctgc
     1501 aaggcagtcg tgaacctgat tcgggccgtc cggccgatca tgaaggctca gggcgggggg
     1561 cggatcgtga acatcgtgac cgagctgtgg aacatgggct cggccgagtg gtctacctac
     1621 ttggccggca aaggggcgat ggtgggcctc tcgcgctccc tggcgagcga gctcggaccg
     1681 gaggggatca ccatcaacat ggtggccccg ggctggatgg ccgacgagaa ggtggacccg
     1741 tcctcggacg cgtcgatccg cttcgggcag acgttgccgg ttcggttcca cggcagtgcc
     1801 gacgagattg gcaatggctg cgtgttcttc ctgagcgacc tggcacggta tgtcacgggt
     1861 gcctatctgc ccgtgacggg cggccgcgtg acacagatgg gcgcgtagcg gacgagaggg
     1921 aaggggcccg tcgagatgag cagacccatc ggtatgggcg tcgtgggggc cggaagcatc
     1981 gggatccgtg gagcgctcca gcacctgtgc ctgcccgacg ttcaggatcg cgtgagactg
     2041 gcagcggtgt gcgatccggt tccggggcgg gcggcggccg ccgccgccaa gtatggagtg
     2101 ccacacgcct accagagctt cgaggagctg ctggccgacg actcggtcga tgcggtcaca
     2161 ctgggatcac ccattggtct gcactatgaa cagggcttgg ccgcggtgcg tgccggcaag
     2221 cacgttcact tcaacaagac gatgacgacg accgcaagtg aggccacgaa gatcatcaac
     2281 gaagccgcgg cccatcgcgt gagactggtt gcatcgcctg ggcagatgct gcggcccatc
     2341 aaccagcgca tccgcaggct cgtgcaggat ggagccctcg gacgcctggc ctgggccgcc
     2401 acgggagcgg gctttggaac gtaccatgag aacgagggat gccgccaggg cgacgatgtg
     2461 ctcagcaaca tcaacccggc atggtactgg cggagacccg ggggcgggcc actctttgac
     2521 atgaccgtgt acgggctgca ctcgcttacg ggcgtgctcg gaccggccca acgcgtggcg
     2581 ggcatgtcgg gagtggccgt gcgcgagcgc gagttccagg ggacgcggta cgcctgcgac
     2641 gccgacgaca acacactgct gttgctggac ttcggcaatc agacctacgc attcgtctac
     2701 ggcgctctct cagggatgct gacggagttc ggcctggcca gctacttcgg tactcgcggg
     2761 cacatcgcgg gccgcacgat gaacggagag ccaatcgact atccgggccg gaccgaggcc
     2821 gaggcggcgg gcggcgacaa cgtgctgttg ccccatgtgg tgggccccca tcgagagctc
     2881 gatgaggccc acgtcttcga ggacatcatg cagctcgtgg actgggctcg cgaggggatc
     2941 ccctcgcccg cgaccgccga gcatgcgcgc catgtaatcg agatcttcga ggccgggtac
     3001 cgagccgctg agaccggcga gactcagacg ctgaccacga ccttcgagct gccagaccag
     3061 gggtgagcgg gcgcggcagg aggatcgtcc ggcgggctgc gcgaagcctt gaggacggta
     3121 tgggggcatg cgggggcgtg actgcaccat ggacaccact gtggggtcgc ctgtgccgaa
     3181 gcgtgcgggc cggctgatct ggttccaggc tgccggcgct gtcgtgacca gcatgggcgc
     3241 gttcctggta ctgtgtctgt gtgtgatcat cccctcgatg cgcgccagtc tgatcgacgg
     3301 caagcgcgaa gcgattgcag accacacctc ggtggccgtg gcgatcctgg cagagtccta
     3361 tgccaaggag cgcgctggca aagcaacacg agaggaagct cagcgcgatg ccctccgtgt
     3421 tcttcgagg
//
LOCUS       DAOSPL010000339         3423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_27726_length_3423_cov_2.502375, whole genome shotgun sequence.
ACCESSION   DAOSPL010000339 DAOSPL010000000
VERSION     DAOSPL010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27726_length_3423_cov_2.502375"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /gene="recA"
                     /locus_tag="PLQ54_07445"
     CDS             complement(<1..732)
                     /gene="recA"
                     /locus_tag="PLQ54_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOS93132.1"
                     /translation="MEVDRNKERALADALAGIEKQYGKGSIMRLGSAPRRAESGVIPT
                     GSLSLDLALGIGGIPRGRIIEVYGQESSGKTTLVLHILANTQKLGGTVAFIDAEHALD
                     PDYARDIGVNIDDLIVSQPDTGEQALEIADALVRSGAIDAVVVDSVAALVPRAEIEGE
                     MGDSHVGLQARLMSQALRKLGGSLSRSNTAAIFTNQVREKIGVVYGNPEVTPGGRALK
                     FWSSVRIEIRRGEALKRGTDILGVRV"
     gene            complement(746..1408)
                     /gene="thpR"
                     /locus_tag="PLQ54_07450"
     CDS             complement(746..1408)
                     /gene="thpR"
                     /locus_tag="PLQ54_07450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOS93133.1"
                     /translation="MSGLPWVREARERGQSRVAEVYRCFIAVPLPPAFREAVAGLQRQ
                     VRDACDTVMGRADMLRWVRPDDMHMTVRFLGDTPTRKMAPLGDLLEQSAARARCVDLS
                     ATGVDAFPSAARPRVLLLRMSDPTDVLSSLAKEIERGVRRLGFTREERPFTPHVTLAR
                     ARRDSRLPSLEGIVSGQEVAGLGPLRVEALVLYRSELAPGGPHYTVLATHTLPQDAVA
                     PA"
     gene            complement(1486..>1728)
                     /locus_tag="PLQ54_07455"
     CDS             complement(1486..>1728)
                     /locus_tag="PLQ54_07455"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOS93134.1"
                     /translation="AMATGVRRLCGSTYGLAATGIAGPAGGSPEKPVGLVYTAVAWPD
                     GVESAEHRFHMDRLDNKQRASQAVLLMLHHRLRRTA"
     assembly_gap    1729..1738
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1739..2867)
                     /locus_tag="PLQ54_07460"
     CDS             complement(<1739..2867)
                     /locus_tag="PLQ54_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOS93135.1"
                     /translation="MVAEIMSVGTELLLGQITDTNATYISQQLAAHGVDVYYRTTFGD
                     NLERVTEGLRLALSRADAVIITGGLGPTTDDVTREAIAAAFGRELVMDESARAHIERF
                     FAARGYAMTSSNLKQALVPQGGELIANPKGTAPGVLVEADGKLVFAVPGVPREMQAMV
                     DEAVIPRLVAALPKSLVIRSRVIRLAGIGESAMAKKVEDILVGQANPTVAPLVGRGDV
                     ILRITAKGESEHEVERSITDVESRIRERLGEYIYAVGEEPLEYWLVQALTERKETLSI
                     AESCTGGLVSDRITNVSGSSACYLGSVVSYDNEVKATVLGVSRDLLAARGAVSAEVAA
                     VDYDGMVREAEVLRKIAKNVAIKVPLTPDGLRTCRRLSDQGT"
     gene            complement(3004..>3423)
                     /locus_tag="PLQ54_07465"
     CDS             complement(3004..>3423)
                     /locus_tag="PLQ54_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93136.1"
                     /translation="APLTLGEGIAVVAPDGLRAVPVATARREGTLPFTFTSLLLPFRG
                     AETPDVVLRELPVEPPGAWARGTWVQWATGEALLVFVAPLESDDPDARYVVRGPDGFD
                     AAMTAACAAIWLRREGDRWQAEETLGVGMRSDSPEAG"
BASE COUNT          582 a         1300 c          983 g          548 t
ORIGIN      
        1 gactctcact ccgaggatgt cggtgcccct cttcagcgcc tcgccacggc ggatctcgat
       61 gcgcacagac gaccagaact tcagggcgcg cccgcctggc gttacctccg gattgccgta
      121 gactacgccg atcttctcgc gaacttggtt cgtgaaaatc gccgccgtgt tcgaccgcga
      181 tagcgaaccg cccagcttcc gtaacgcctg cgacatcagc cgcgcctgca ggcccacgtg
      241 cgagtccccc atctcgccct cgatctccgc tcgggggacc aacgcggcga cggaatccac
      301 caccaccgca tcgatggcgc cgctccgtac cagcgcatcg gcgatctcca gtgcctgctc
      361 gcccgtgtct ggctgggaga cgatcaagtc gtcgatgttc acgccgatat cccgggcgta
      421 gtcggggtcc agcgcgtgct cggcgtcgat gaaggccacg gttccgccca gcttctgggt
      481 gttcgccagg atgtggagca ccagtgtcgt cttgcccgac gactcctggc cgtatacctc
      541 gatgatgcgt ccccggggaa tgccgccaat ccccaacgcc aggtcgagcg atagcgaccc
      601 cgttgggatc accccgctct cggctcggcg cggcgccgaa cccagccgca tgatgctgcc
      661 cttgccatac tgcttctcga tccccgccag agcgtcggcc agcgcgcgct ccttgttgcg
      721 atccacttcc atcagggctt cctcctcagg ctggggccac cgcgtcctgc ggcaacgtgt
      781 gtgtagccag aacggtgtag tgcggccccc cgggcgccaa ctcgctccga tacagaacca
      841 acgcctccac ccgcagcggc cccagacccg cgacctcctg gccactcacg atgccctcca
      901 gcgacggcag acggctgtca cggcgtgccc tcgccagcgt cacgtgtggg gtgaatggcc
      961 tctcctcccg ggtgaagccc agccggcgaa cgcccctctc gatctccttg gccagagagg
     1021 acaacacgtc cgtcgggtcg gacatccgca acaacagtac ccgagggcga gccgcggagg
     1081 ggaaagcatc cacgcccgtg gcagacaggt ccacgcaacg cgcgcgggcc gcgctctgtt
     1141 cgagcagatc cccaaggggc gccatcttcc gcgtgggcgt atcgccgagg aaccgtacgg
     1201 tcatgtgcat atcgtccggg cggacccagc gcagcatgtc agcgcggccc atgaccgtat
     1261 cacaggcgtc ccgaacctgc cgctgcagcc cggcaaccgc ctcacggaag gccggcggta
     1321 gcggtaccgc gatgaagcac cgatagacct cagccactcg gctctgccct cgctcgcgag
     1381 cttcgcgcac ccaaggcaac ccgctcatcg gcacccttca ccaggagccg tgcggtagcc
     1441 gagcacaggc actctgccgc ccgggctgga ccccgggcag acacttcagg cggtccttct
     1501 caggcggtgg tgaagcatga gaagcacggc ttgtgaggcc ctctgtttgt tgtccagtcg
     1561 atccatgtgg aagcgatgct ccgccgactc caccccatcc ggccacgcca cggccgtata
     1621 caccagtccc accggcttct cggggctgcc ccctgccggc ccggcgatgc ccgtggcagc
     1681 caggccatac gtgctgccac acagcctccg gacgcctgtc gccattgcnn nnnnnnnntc
     1741 gtgccctgat cgctcagccg gcggcaggtg cgcagcccgt cgggcgtcag cggcaccttg
     1801 atcgcgacgt tcttggcgat cttgcgcagc acctcagcct ctcggaccat gccgtcataa
     1861 tcgacggcgg ccacttccgc gctgacggca ccgcgtgccg ccagcaagtc gcgactcacc
     1921 ccaagtacgg tcgccttcac ctcgttatcg tagctcacca cggaacccag atagcacgct
     1981 gagctcccac tgacgtttgt gatgcggtcc gaaaccaacc cacccgtaca cgactccgcg
     2041 atcgacagcg tctctttccg ctccgtcaga gcctgcacga gccaatactc cagcggctcc
     2101 tcgccgacgg catagatgta ctcgcccagc cgctcgcgaa tccgcgactc cacgtcggta
     2161 atcgaccgct caacctcgtg ctcggactct cccttggccg tgatccgaag gatcacatcg
     2221 cctcggccca ccagtggagc cactgttgga ttggcctggc caaccagaat gtcctccacc
     2281 tttttcgcca tggcagactc gccgatcccc gccaggcgaa tcaccctgga ccggatcacc
     2341 aggctcttgg ggagtgccgc aacgagcctc gggatcacag cctcgtccac catggcctgc
     2401 atctctcgcg gcacacctgg taccgcgaac acgagcttcc catccgcctc aaccagtaca
     2461 ccgggtgccg tacccttcgg gttggcgatc agctcgccac cttgggggac gagcgcctgt
     2521 ttgagattgg agctcgtcat cgcatacccg cgcgccgcaa aaaaccgctc gatatgagcc
     2581 cgagcactct cgtccatcac cagctctcgc ccgaaggcgg ccgcgatggc ctcccgtgtc
     2641 acgtcatccg tggtcggccc gaggccaccg gtgatgatga ccgcatcagc ccgtgagagg
     2701 gccagacgaa gcccctcggt gacccgctcg aggttgtcac caaaagtggt ccgatagtag
     2761 acatccaccc catgcgccgc gagctgctgg ctgatgtacg tggcattcgt atccgtgatc
     2821 tgccccagca gcagctccgt acccacagac atgatctcgg cgaccaaggg tccatcacct
     2881 aacacgtgcc ggccgccggc cacgggcgag cggggacgca cgaacgttgc ccgcattata
     2941 ggtagggacg ccccagccca caacggcccc tgccccggtg ccgcccgcaa cgccggccgt
     3001 ggcctatccc gcctccggcg agtcagacct catacccact ccgagcgtct cctcggcctg
     3061 ccaccgatcc ccctcacgcc gtagccatat ggccgcgcag gcggcggtca tggccgcatc
     3121 gaagccatcc ggtcccctga ccacataccg ggcatcgggg tcgtccgact ccagcggcgc
     3181 cacgaagacg agcagcgcct cgcccgtcgc ccactgcacc cacgtccccc gagcccaggc
     3241 gccgggcggc tccaccggca gttcccgcag cacgacgtca ggcgtctcag ctccgcgaaa
     3301 cggcagcagc agggatgtga atgtgaacgg gagtgtacct tcgcgcctcg ctgtggccac
     3361 gggcaccgcc cggaggccgt ccggagccac gaccgcgatc ccctcaccca gtgtcagcgg
     3421 cgc
//
LOCUS       DAOSPL010000340         3403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_27938_length_3403_cov_3.262545, whole genome shotgun sequence.
ACCESSION   DAOSPL010000340 DAOSPL010000000
VERSION     DAOSPL010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27938_length_3403_cov_3.262545"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLQ54_07470"
     CDS             complement(<1..611)
                     /locus_tag="PLQ54_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93137.1"
                     /translation="MRSRVMFCAAVGLALMLGGTAWAQLVPNQGGGAAAGGSAGGGLM
                     RGPGGGGINPMMMGGSGGGMMMGGMGGMMGAGMQQTADPKKARRLMNEALALLEKADK
                     QMEDGNLQAARDAYKQAKEKMDEVRGTSSKSQAGPGGGMGGGAMPGMMGAGAMAGGAA
                     GSGGGMMPGGGAMMGGGMMGGGMGGGGLIAHDLAPENQKLLQIL"
     gene            complement(923..1996)
                     /locus_tag="PLQ54_07475"
     CDS             complement(923..1996)
                     /locus_tag="PLQ54_07475"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS93138.1"
                     /translation="MTPACGGPIRVGLAGYGLGGRVFHAPLIRRVPGLQLTAVMSRSE
                     DKRRQAEQELGVRTFDHFSTLANSPETDLVVIVTPHDTHCALAVEALRAGKHVIVDKV
                     MSLTVAEADRMIEAAENANRLLSVYQNRRWDGDFLTLRRVVDEGLLGRLRVVESCVGR
                     WGLSRRTAWRLSDAHGGGYFRDWGAHLVDQALQIGGTPRRVFGDMLFTDPSVDIETYG
                     YARIEFASGLRFCVETGCLNRAPKPRFWARGDLGSFVKYGLDPQEAALARGDCGWPGD
                     DPAHRALLHVDVGGQPAEVLVETVPGGYVRYYENIAAVLHEGAVLAVTAAQGREVVRV
                     LEAARQSAHTGAWVRLDASPSGA"
     gene            complement(1993..2691)
                     /locus_tag="PLQ54_07480"
     CDS             complement(1993..2691)
                     /locus_tag="PLQ54_07480"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018760912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HOS93139.1"
                     /translation="MPELMDSLRGRLIVSCQAAPDSPLGKPETLAALAECAVLGGAAA
                     IRADGPENIRAITGRVDVPVLGIYKKHYEGFEPYITPTVAEAMAVIEAGAKLLAVDAT
                     PRPRPGACALADIVTVAHDCGVPVMADCSAVDDARFAQDAGADLLGTTLAGYTPYSRQ
                     IAGPDFELIGEIAEVCDRPVVAEGRIHSPAEAARALIEGAFAVVVGTAITAPQAITSW
                     YANACAAAAEAVAK"
     gene            complement(2755..>3403)
                     /locus_tag="PLQ54_07485"
     CDS             complement(2755..>3403)
                     /locus_tag="PLQ54_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOS93140.1"
                     /translation="VLLGDEGSGFSLGWEAIRATMRALDGRAPATLLATAVCRHLLGH
                     ADPIPDSGIDGSHRRDLAGRIRLATLESSRAKVAELAPAVSAAAAAGDAVARGILEAA
                     GRELALNAESLLRGWSEARPPRVGGLGGVFRAGPPLTHAFRDGVARSAPGAEARILEV
                     DLVAGAVLLVLEGRPDAQKHVLDQWPATRTRIAALPRLTAPADEPSLRSMLGVIR"
BASE COUNT          496 a         1207 c         1162 g          538 t
ORIGIN      
        1 aggatctgca gcagcttctg gttctcgggg gccagatcgt gggcgatgag cccgccgccc
       61 cccataccgc cgcccatcat gccgccaccc atcatggcgc cgccacccgg catcattccg
      121 ccgccggacc cggcggcgcc cccggccatg gccccagcgc ccatcatccc aggcatggcg
      181 ccgccaccca tgccgccacc gggacccgcc tgcgacttcg acgacgtgcc gcggacctcg
      241 tccatcttct ccttggcctg cttgtaggca tcgcgggcgg cctggaggtt gccgtcctcc
      301 atttgcttgt cggctttctc gagcaacgcg agggcctcgt tcatgagccg gcgggccttc
      361 ttggggtcgg ccgtctgctg catgccggcg cccatcatgc cgcccatacc gcccatcatc
      421 atcccgccgc ccgagccgcc catcatcatg gggttgatgc caccgccacc agggccacgc
      481 atcaggccgc cgccggcgga gccccccgcg gcggcgccgc ccccttggtt cggcacaagc
      541 tgcgcccagg cggttccgcc cagcatgagt gccaggccca ctgcggcgca gaacatcact
      601 ctcgaacgca tcggcgctcc accctctccc ggctgccgtc ggtacggcag cgacccaagt
      661 cagccagcag aggcagacag cgacacagac accggctgtc gagccaggct ctctgcgctg
      721 cccgcaccat cttgtccagc acgctctcgc gatcgtaccc ggctgcccgc cgtgcctttt
      781 tggactccta atcgggcttg ccggttcctg ctacggggag gttcgtaggc ttatggcacg
      841 actccccgtg tccggatatt tcaaggtccg catctggcgt tccttctcgg ccaggactaa
      901 ggtttcccgg gtccaggccg ttctatgcac cggatggact ggcgtctagc ctgacccacg
      961 ccccggtgtg ggcagactgt cgtgccgctt cgaggacgcg cacgacctcg cgcccttggg
     1021 cggccgtgac cgcgagcacc gctccctcgt gcagcaccgc ggcgatgttc tcatagtacc
     1081 gcacgtaccc tcccggcacg gtctcgacga gcacctcagc cggttggcct cccacgtcga
     1141 catggaggag cgcccgatgc gccggatcat cgcccggcca accgcagtcg ccacgggcga
     1201 gcgccgcttc ttgcggatct agaccatact taacgaacga acccaggtcg ccccttgccc
     1261 agaaccttgg cttgggcgcg cggttcaaac agcccgtctc gacacagaag cgcaggccgc
     1321 tggcgaactc gatccgggcg tacccgtagg tctcgatgtc cacggacggg tcggtgaaga
     1381 gcatatcacc gaacacgcgc cgcggtgtgc cgccgatctg cagtgcctgg tcgaccaggt
     1441 gggctcccca gtcgcggaag tatccgccac cgtgcgcgtc ggagagccgc caggccgtgc
     1501 gccggcttag gccccatcgg cccacgcacg attcgacgac gcgcagacgc ccgagcagcc
     1561 cctcgtcgac gacgcggcgc agggtgagga agtcaccgtc ccatcgccga ttctggtaga
     1621 cgctgagaag ccggttggcg ttctccgccg cttcgatcat ccggtcggcc tcggccacgg
     1681 tcagggacat gaccttgtcg acgatcacgt gcttgccggc acggagcgcc tcaaccgcca
     1741 gggcgcagtg ggtgtcgtgc ggggtgacaa tcacgaccag gtcggtctca gggctgttgg
     1801 cgagggtgga gaagtggtcg aaggttcgaa cgcccagttc ctgctcggct tggcggcgct
     1861 tgtcctcgct gcgcgacatg actgcggtga gttggaggcc cggcactcgg cggatgagcg
     1921 gagcgtggaa cacgcgcccg ccgagcccgt agccggccag gccaacgcgg atgggcccac
     1981 cgcatgcggg ggtcatttgg ccaccgcctc ggcggccgcg gcgcaggcgt tggcgtacca
     2041 tgacgtaatg gcttggggcg ccgtgatggc cgtgcccacg acgacggcga aggcgccctc
     2101 gatcagcgct cgggctgcct cggcgggcga gtggattcgg ccttcggcca cgacgggacg
     2161 gtcgcacact tccgcgatct cgccgatgag ttcgaagtcg gggccggcga tctggcgcga
     2221 gtagggggtg tagcccgcca gcgtcgtgcc caacagatcg gcaccggcgt cttgggcgaa
     2281 ccgggcgtca tcgacggccg agcagtcggc catcacaggg acgccgcagt cgtgggccac
     2341 ggtcacgatg tcagccaggg cgcacgcacc gggccggggc ctgggtgtgg catcgacggc
     2401 caagagtttg gcgccggcct cgatgacggc catggcctcc gcaaccgtgg gcgtgatgta
     2461 gggctcgaag ccttcgtagt gcttcttgta tatgccgagg accggcacgt ccacgcggcc
     2521 ggtgatggcc cggatgttct cggggccatc ggctcggatg gcggccgcgc cacccaggac
     2581 ggcacactcg gccagagccg ccagtgtctc gggcttgccc aggggtgagt ccggtgctgc
     2641 ctggcatgag acgatcagcc tgccgcgcag cgagtccatg agctcgggca tcggatggca
     2701 cctctctcag cgcgtgagtg catatggtgt gtgccacgca gggccgtatc cccgctagcg
     2761 tatcactccg agcatggatc ggagcgaggg ttcgtcggcc ggagcggtca gtcggggcag
     2821 cgccgcgatg cgggttcgag tcgcgggcca ctggtccagc acgtgtttct gggcgtctgg
     2881 ccggccctcg agaacgagga gcacggcgcc ggccaccaga tcgacctcaa ggattcgagc
     2941 ctcggcgccc ggggccgagc gtgccacgcc gtcgcggaag gcatgggtga gcggaggccc
     3001 ggcacggaag acccctccga ggccgccgac gcgggggggg cgggcttcgg accagcctct
     3061 gagcagactc tcggcattga gcgccagctc gcgcccagcg gcctcgagga tgccgcgcgc
     3121 cacggcgtcg ccggctgcgg ccgctgccga cacggcgggg gccagttcgg ccacctttgc
     3181 gcggcttgat tcgagtgttg ccagccggat gcgacccgcg aggtcgcgcc ggtggctgcc
     3241 gtcgatgccg ctgtccggga tcggatccgc gtggcccagg aggtgccggc agacggccgt
     3301 tgccagcaat gtcgcgggtg ctcggccgtc cagcgcgcgc atggtggcgc gaatggcttc
     3361 ccagccgagg ctgaagccgc tgccctcgtc gcccagaagc acg
//
LOCUS       DAOSPL010000341         3390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28073_length_3390_cov_2.925637, whole genome shotgun sequence.
ACCESSION   DAOSPL010000341 DAOSPL010000000
VERSION     DAOSPL010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28073_length_3390_cov_2.925637"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="PLQ54_07490"
     CDS             <1..1097
                     /locus_tag="PLQ54_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOS93141.1"
                     /translation="VAALFIGAASLRNAALAIEVAREMGLLGEDILGTGFSFDLDIVT
                     GAGAFVCGEETALIASIEGFAGEPRPRPPFPAQRGLFGKPTNINNVETWANIPLIIRH
                     GAAWFSEIGYERSRGTKIFSLVGEVQNTGLVEVPVGMTLNEIVFEVGGGPKPGRTIKA
                     VQTGGPSGGCIPASLFDVRVDYESLAQVGSIMGSGGLIVMDDRTCIVDVARYYLRFLK
                     SESCGKCTPCREGVPRMLEVLDRIASGEGSDADIDLLGQLGRTVKGASLCGLGQTAAN
                     PVLSSIRHFRHEFDAHIHEKRCPAAVCTALITFHIDAEKCTGCTACARACPQQCITGE
                     RKQPHVIDPERCIKCGACLDACRFDAVVKE"
     gene            1120..1869
                     /locus_tag="PLQ54_07495"
     CDS             1120..1869
                     /locus_tag="PLQ54_07495"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOS93142.1"
                     /translation="MIRIEIDGNAVEVAEGSTVLEAARKLGIDIPTLCHHAGLTPYGA
                     CRLCVVEVEQRGRKRIESSCTRPVEPDMVVRTDTDDLREYRRTTAELLVAQCPDSKPI
                     RELAERLGVQNVRFEGRGMECILCGLCTRACQQKIGTPAISFIHRGVDRKVATPFEIQ
                     SDVCIGCGACARVCPTGCIAIEDMGNKRVLRVFNTELELQACSECGAYFAPQRMIDRL
                     KTETEVTEGLLTRCPACRRQAIGAVLRQAMR"
     gene            1876..2418
                     /locus_tag="PLQ54_07500"
     CDS             1876..2418
                     /locus_tag="PLQ54_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOS93143.1"
                     /translation="MLLRPLERSSNEGPELKRVYLVPNRCLGCEECIEACKREHDWVA
                     CNYIEWVNRIYPVQMVCNHCAEAPCELICPAGAIHRTETGAVVVEDDKCIGCTLCNIV
                     CPFGIPKFSEEDRHAIKCDQCAERVAAGKEPACVEACPMKALTFAEVEDYEAERRERT
                     ARRILEVGTCLRRFVAKKES"
     gene            2422..2814
                     /locus_tag="PLQ54_07505"
     CDS             2422..2814
                     /locus_tag="PLQ54_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93144.1"
                     /translation="MGAQTEAVPYEEIVAQLEPDEKVALVTCNTCVRVCETGGEAFME
                     EMARRLRADGFEVTDEVVTITTCNRDYVFDAPMSGRQTATIVLACDVGWMAVVQRFRD
                     RKCVKACKTTGLMSSVPKSRPKAGAAAS"
     gene            2836..3357
                     /locus_tag="PLQ54_07510"
     CDS             2836..3357
                     /locus_tag="PLQ54_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93145.1"
                     /translation="MVVSLLKLTYNELVEQLNDDDRIVVWTCNDCVRYCGLGGRDPLD
                     ALADRLEADGYNVIHRELCGISCNRGLVRNRQTHPATKPIYQDATVIIPLVCREGLET
                     LAFVFKKQRIIDTVETLGLGVYSEETGMRLNVPLEETGLEPSIDGYDLAELAEQRGLH
                     TGPFVAGQKAKAK"
BASE COUNT          583 a         1095 c         1166 g          546 t
ORIGIN      
        1 gcgtggccgc gctgtttatc ggtgctgcat cgctgcgcaa cgcggcgctg gccatcgagg
       61 tcgctcgcga gatgggctta ctcggtgagg acatcctcgg cacggggttc tcgttcgatc
      121 tggacatcgt caccggcgcg ggtgccttcg tctgcggcga ggagaccgct ctcatcgcat
      181 ccatcgaggg cttcgccggc gaaccgcggc cccggccacc gtttccggcg cagcgcggcc
      241 tgttcgggaa acccaccaac atcaacaacg tcgagacttg ggccaacata ccgctcatca
      301 ttcgccacgg cgcggcctgg ttcagcgaga tcggctacga gcgcagtcgc gggaccaaga
      361 tcttctctct cgtcggcgag gtccagaaca cgggcctggt tgaggtgccc gtaggcatga
      421 cgctgaacga gatcgtcttc gaagtgggtg gcggcccgaa gccgggccga acgatcaagg
      481 cggtgcagac gggcggcccc tcgggcggct gcatccccgc atcgctcttc gatgtgcgtg
      541 tcgactacga gagcctggcg caggtgggct ccatcatggg ctcgggcggc ctgatcgtga
      601 tggatgaccg cacctgcatc gtggatgtgg cccgctacta cctccgtttc ctgaagtccg
      661 agtcgtgcgg caagtgcacg ccgtgccgtg aaggcgtgcc ccggatgctg gaggtgctcg
      721 accgcatcgc gtccggcgag ggctccgacg ccgacatcga cctgctcggc caactcggcc
      781 gcacggtcaa aggcgcatca ctgtgtgggc tcggccagac ggccgccaac ccggtactga
      841 gcagcatccg gcacttccgc cacgagttcg atgctcacat acatgagaag cgctgcccgg
      901 cggcggtgtg cacggcactc atcacgttcc acatcgacgc cgagaagtgc acgggctgta
      961 ccgcatgcgc ccgggcgtgc ccacagcagt gcattacggg ggagcgcaag cagccccatg
     1021 tgatcgaccc ggagcggtgc atcaagtgcg gcgcgtgcct cgacgcgtgc cgcttcgacg
     1081 cggtagtgaa ggagtaggcc tctggtggag gccctggcga tgatccgtat cgagatcgac
     1141 ggaaatgcag tggaagtggc cgagggctcc acggtgctgg aggccgctcg aaagctcggc
     1201 atcgacatcc cgaccctgtg ccatcatgcc gggctgacgc cctatggcgc gtgccggctg
     1261 tgcgtggtcg aggtcgaaca gcggggccgg aagcgcatcg agagctcctg cacgcgtccc
     1321 gtggagccgg acatggtcgt gcgcaccgat accgacgacc tgcgcgagta ccgtcggacc
     1381 acggccgagt tgctcgtggc ccagtgcccg gactcgaagc ccatccgcga gctggccgag
     1441 cgtctggggg tccagaacgt gcgcttcgag ggccgcggga tggagtgcat cctctgcgga
     1501 ctctgcacgc gggcttgcca gcagaagatc ggcacaccgg ccatcagctt catccatcgg
     1561 ggagtggacc ggaaagtggc cacgcccttc gagatccagt ccgatgtgtg catcggttgc
     1621 ggcgcgtgcg cccgcgtgtg cccaacgggc tgcatcgcca tcgaggacat ggggaacaag
     1681 cgcgtcctgc gcgtcttcaa caccgagctc gagctacagg cctgctccga atgtggggcc
     1741 tacttcgccc cccagcggat gatcgaccgg ctcaagacag agacggaggt caccgaaggc
     1801 ctgttgaccc ggtgcccagc ctgccggcga caggcgatcg gcgcggttct ccgacaggcc
     1861 atgaggtagt gcgccatgct gctccgcccc ctggagcgga gctcgaatga gggacccgag
     1921 ttgaagcgag tctacctggt gccgaaccga tgcctcgggt gcgaggagtg catcgaggcc
     1981 tgcaagcggg agcatgactg ggttgcctgc aactacattg agtgggtgaa ccgcatctac
     2041 cccgtgcaga tggtgtgtaa tcactgcgcc gaggcgccct gcgagctgat ctgcccggcg
     2101 ggcgccatcc atcgcaccga gaccggcgcc gttgtggtcg aggacgacaa gtgcatcggc
     2161 tgcacgctgt gcaacatcgt gtgccctttc ggtatcccca agttcagtga ggaagaccgg
     2221 cacgcgatca agtgcgacca gtgcgccgag cgtgtggccg cgggcaagga accggcatgt
     2281 gtcgaggcgt gccccatgaa ggccctgacc ttcgccgagg tcgaggacta cgaggccgag
     2341 cgccgcgagc gcacggcccg gcggattctt gaggtgggca cctgcctgcg acggttcgtt
     2401 gcgaagaagg agagctaggc gatgggagcc cagaccgagg ccgtccccta tgaggagatc
     2461 gtggcccagc tcgagccgga cgagaaggtc gcgctggtga cgtgcaacac gtgcgtgcgc
     2521 gtatgcgaga ccggcggcga ggcgttcatg gaggagatgg cccgccgcct ccgcgccgac
     2581 ggattcgagg tcaccgacga agtggtcacc atcaccacct gcaaccgcga ctacgtcttc
     2641 gatgccccca tgagcggccg tcagaccgcg acgatcgtcc tcgcctgcga cgtgggctgg
     2701 atggccgtcg tccagcgctt ccgcgaccgg aagtgcgtca aggcctgcaa gaccacgggc
     2761 ctgatgtcga gtgtccccaa gtcccggccc aaggccggcg ccgcggcctc gtagcggcgg
     2821 gttggaggag tccgggtggt cgtctctcta ctcaaactga cctacaacga gctggtcgag
     2881 cagctcaacg atgacgaccg gatcgtcgtc tggacctgca acgactgcgt ccgctactgc
     2941 ggcctgggtg gccgcgatcc gctcgacgcc ctggccgacc ggctcgaggc tgatggttac
     3001 aacgtcatcc accgggagct ctgcggcatc tcgtgcaacc gcggcctcgt gcgcaaccgc
     3061 cagacgcatc cggccacgaa gccgatctac caggatgcga cggtcatcat cccgctggtc
     3121 tgccgcgagg gtctggagac gctggccttc gtgttcaaga agcagcgcat catcgacacg
     3181 gtcgagacgc tggggctcgg cgtgtactcc gaggagaccg gcatgcgcct caacgtcccg
     3241 ctcgaggaga cgggcctcga accgtcgatc gacggctacg acctcgccga actggccgag
     3301 cagcgtgggc tacacaccgg gccgtttgtg gcgggccaga aggccaaggc gaagtagagg
     3361 gccacgtctg cggggggctg gatcggtgcg
//
LOCUS       DAOSPL010000342         3388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28091_length_3388_cov_3.432643, whole genome shotgun sequence.
ACCESSION   DAOSPL010000342 DAOSPL010000000
VERSION     DAOSPL010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28091_length_3388_cov_3.432643"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PLQ54_07515"
     CDS             complement(<1..619)
                     /locus_tag="PLQ54_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93146.1"
                     /translation="MRAAFLTMTTIAIVSVLSLSGCGGSPPAGNNGNNNGGVPTAVIV
                     VTEGDTVVAGSTLHLAGEQSQPSAGQTITGYQWTVEAPNGAAGAFVPSAQAANPTFVV
                     DVVGQYTFRLTVFQGATSSATPAEAVVQAQAPTTPVPVISVQEGLQVAPLTTLHLSAA
                     QSVPVPGGTIDRWEWTVEGPAGSVSTLSPGANVQSPTLEANVAGSY"
     gene            835..2103
                     /locus_tag="PLQ54_07520"
     CDS             835..2103
                     /locus_tag="PLQ54_07520"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS93147.1"
                     /translation="MSDATSGQGPNVSRRRFIQAVGTTAASVAAGLHIGAPARAQSRS
                     ANGRLGVGFIGCGGRMGAHIGAVQQLKNEGQPLDLVAVSDVYKPRVDAAVQATGGKPY
                     RDYRELLEDRHVDLVCIASPDRHHGPQALDAVEAGKHVYCEKPMTHWSQFEVAKKLAR
                     RVKERGVAFQLGTQRLADSIWRQGAELIRQGVIGKPLHAQVGYYRQGDWGERGMPILD
                     PSAKPGPDLDWKAFLGDAPEVEFSVQRFFCWRMYLDYAGGPPTDLYPHVFTPLATVLG
                     LGMPTRVSAAGGKLFYNHEREVPDTVNILMDYPQGLTVACMGTQVNAWEMEWVIRGAE
                     GTMTFSGPGIQIFPADGRREPVKEVAREASGDVREMWLNLIECIHSGGKPWSPVDMGY
                     CVQTAMNMGMLSLFEGKTAHFDTDRERIRI"
     gene            complement(2276..>3388)
                     /locus_tag="PLQ54_07525"
     CDS             complement(2276..>3388)
                     /locus_tag="PLQ54_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93148.1"
                     /translation="DLSTGKLLGLLYGVARIYALTWAPDGSELAMCTDEGAWLVPTDG
                     SARARPIGSSKTGDAAFPASGRYVGMGFHPPFDRTEALVLDRQEGTYYHIPCSSSTEL
                     AWAGDRLVRLTGGAKPCLEELRLDSGAASQAWLAWAAAESAPPLGWNTLGPTRRLLAA
                     GDRIFAAPIDPRRAAVTDTAAVVLRVDLVSRRVWQAPLGIRVMDRLPEFSVSPDGRWL
                     AVTFTGVDEEAARMDPAAADGPRLLLFEAVDDPRGLVRRGCIALSGTGVTAVRPSWSP
                     DSHYMVVRGLDLVDIKTRRIVCGLHDRVASQIGYAEWTPPHGLIVTRVGPDESGIYLT
                     QPDSPGARLLFETQPIMPARGQSFVERLLRALRGGP"
BASE COUNT          544 a         1149 c         1189 g          506 t
ORIGIN      
        1 ggtagctgcc ggccacgttc gcctcgagcg tcgggctctg gacgttggcg cccggcgaga
       61 gcgtggagac cgaacccgcc ggtccttcga ccgtccactc ccacctgtcg atggtgccgc
      121 cgggaacggg caccgactgg gcggcactta ggtgcagcgt ggtgaggggc gcgacctgga
      181 gtccctcctg gacggagatg acggggaccg gcgtcgtggg cgcttgggcc tggacgacgg
      241 cctcggccgg ggtggccgag ctcgtggcgc cctggaacac ggtgaggcgg aaggtgtact
      301 gccccaccac atcgaccaca aaggtggggt ttgcggcctg cgccgacggc acgaaggcac
      361 cggcggcgcc gtttggtgcc tcgacggtcc actgatatcc cgtgatggtc tgccccgcgg
      421 aaggttggga ctgctcgccc gccaggtgga gggtcgaccc ggcgacaact gtgtccccct
      481 cggtcaccac gatgactgcg gtcggcacgc cgccgttgtt gttgccgttg ttgcccgcgg
      541 gcggactgcc gccgcagcca ctgagggaca agacgctgac gatggctatt gtcgtcatgg
      601 tgaggaatgc agctcgcact ggcacatcac ccccatgcac tggataggtc gtggcttccg
      661 tacggcgagt ggaatacctc taaggtcgcg agtcattaag cactcgacac gccacggccg
      721 cgcgggtagc gaatgctgcc gcatgggggg ccatgcggcg ggaggccccg gtcgcgcgac
      781 aggcgaactt cgccctcacg cccgatgccg ggcgcccatg gggaggggta catcatgagc
      841 gatgcgacgt caggtcaggg tccgaacgtg tcccgtcgcc ggttcatcca agccgtgggg
      901 acgaccgcgg ccagtgtggc ggccggcctc cacatcggcg cgcctgcccg cgcccaatcc
      961 cggagtgcga atgggcggct gggtgtcggt ttcattgggt gcggcgggcg catgggcgcg
     1021 cacatcggtg ccgttcaaca gctgaagaac gagggccaac ccctcgacct cgtggcggta
     1081 tcagatgtgt acaagccccg cgtcgatgcg gcggtgcaag ccacgggagg taagccctac
     1141 cgcgactacc gcgagctact cgaagatcgc cacgtcgatc tcgtgtgcat cgcctcgccc
     1201 gaccgacacc acgggccaca ggcgctcgat gctgtcgagg ccggcaagca cgtctactgc
     1261 gagaagccca tgacgcactg gagccagttc gaggtcgcca agaagctggc ccgacgggtc
     1321 aaggagaggg gcgtcgcctt ccagctcggc acccagcggc tggccgatag catctggcga
     1381 cagggggcgg aactgattcg gcagggagtc atcggcaaac cgctgcacgc ccaggtcggc
     1441 tactaccgcc agggtgactg gggcgagcgc ggcatgccca tcctcgaccc gagcgccaag
     1501 cccgggccgg atctcgactg gaaggccttt cttggcgatg cgcccgaggt cgagttctcg
     1561 gttcagcgct tcttctgctg gcgcatgtac ctcgactacg ccggaggccc gcccactgac
     1621 ctctatcccc acgtgttcac tccgctggcc acggtgcttg gactcggcat gccgactcgt
     1681 gtctcggccg cgggcgggaa gttgttctac aaccacgagc gggaggtgcc cgacacggtg
     1741 aacatcctga tggactatcc gcagggcctc accgtggcct gcatgggcac ccaggtgaat
     1801 gcgtgggaga tggagtgggt gatccgtggc gccgagggca ccatgacctt ctcgggcccc
     1861 ggcatccaga tcttccccgc cgacggccgg cgcgagcccg tcaaggaagt cgcccgagag
     1921 gcctccggcg acgtccggga gatgtggctc aacctgatcg agtgcatcca ctcgggcggt
     1981 aagccctgga gccccgttga catgggctac tgtgtccaga ccgccatgaa catgggcatg
     2041 ctgtcgctgt tcgagggcaa gacggcgcac ttcgacaccg acagggagcg gatcaggatc
     2101 tagcgcgcgc agcccgaggc caagacggcg gggccccggc cgctggcgcc cggccggtcg
     2161 cagacgcggc cctccgtttc gctggtgctg cgctcaggct gacatactcg cacggacggc
     2221 gggagtaccg gggccgtggg cgcgcgcggg caacgtgtcc agtcactgcc gcctcctacg
     2281 gcccgccgcg caacgctcgg aggagccgct ccacgaacga ctggccccgc gccggcatga
     2341 tgggctgggt ctcgaacagg agccgggctc ccggcgagtc gggttgcgtc aggtagatcc
     2401 ccgactcatc cgggcccacc cgggtgacga tgaggccatg gggcggagtc cactcggcat
     2461 acccgatctg gctcgcgacg cgatcgtgaa ggccgcacac gatccgccgc gtcttgatgt
     2521 cgaccaggtc aagccctcga acgaccatgt agtgcgagtc gggcgaccac gaggggcgca
     2581 cggccgtgac cccggtccct gagagtgcga tgcatcctcg ccgcacgagc ccccgcgggt
     2641 cgtcgacagc ctcgaacagg agcagccgcg gcccgtcagc tgccgcgggg tccattcgag
     2701 ccgcctcttc gtcgacgccc gtgaacgtca cggcaagcca ccgaccgtcc ggtgatacgc
     2761 tgaactcggg gagccggtcc atcaccctga tcccgagcgg cgcctgccac acgcggcggg
     2821 acacgaggtc gacccgcagg acgacggccg cagtgtccgt gacggcggct cggcgcggat
     2881 cgatgggcgc cgcgaagatc cggtcacccg cagccagcag tcgccgtgtg ggccccaggg
     2941 tattccaacc cagcggcggg gcagattcgg ccgccgccca agccagccac gcctgtgatg
     3001 ccgcgccact atcaaggcga agctcctcaa ggcagggctt ggctccgccc gtgagtcgca
     3061 cgagccggtc gcccgcccag gccagctcgg tgctggagct gcaggggatg tggtagtagg
     3121 tgccctcctg gcggtccagc accagggcct cggtgcggtc gaaaggcggg tggaagccca
     3181 tgccgacata ccggccggat gcggggaatg cggcatcccc ggtcttgctg ctgccgatgg
     3241 gtcgggcccg ggcagagcca tcggtcggca cgagccacgc gccctcgtcc gtgcacatcg
     3301 ccagctccga gccgtccggc gcccaggtca gcgcgtagat gcgagctacg ccgtagagca
     3361 ggccaagcag cttccctgtg ctcagatc
//
LOCUS       DAOSPL010000343         3388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28094_length_3388_cov_2.877288, whole genome shotgun sequence.
ACCESSION   DAOSPL010000343 DAOSPL010000000
VERSION     DAOSPL010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28094_length_3388_cov_2.877288"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            430..1392
                     /locus_tag="PLQ54_07530"
     CDS             430..1392
                     /locus_tag="PLQ54_07530"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOS93149.1"
                     /translation="MCDRCEQDHGGDVGCDPNCTARCAPPGQVDRRRFILATSRVALA
                     AAVGLSGVRDLAWGQQALQTTKNALGMELTLIAPGTFRMGSEDNDDEKPPHDVTISKP
                     FWLAVCETSNADYRRFVEAAGHGEPGVAVPSKKGIVPWKNPMYRADEQPVVCVTHDDA
                     VAFCAWLSETEGATYRLPTEAEWEYAYRGGTTSRFYWGDEPAGREAAYFSKPWPEETQ
                     QAPGYQEIPWGLLSVLPVSCEERNAHGLCHMAGNVWEWVADWYGPYAADAQTDPIGPA
                     EGTQRVTRGGSRFHASRVATASVRRPMDPDTCCHNRGFRVVREA"
     gene            1513..2565
                     /gene="arsB"
                     /locus_tag="PLQ54_07535"
     CDS             1513..2565
                     /gene="arsB"
                     /locus_tag="PLQ54_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOS93150.1"
                     /translation="MPSVAKQLSVLDRYLTAWIFAAMVLGVALGWAAQSTVEAFNQAT
                     QVGTTSLPIAVGLILMMYPPLAKVRYEAMGPVFRDVKVLALSLIQNWVIGPVLMFALA
                     VLLLRDKPDYMVGLIMIGLARCIAMVIVWNDLAQGDREYCAALVAFNSVFQVVFYSIY
                     AYVFVTVLPPLVGLAGARVEVTIGQIAKSVFIYLGIPFLAGIITRFSLLRLKGLDWYE
                     HRFVPRISPITLVALLFTIVVMFSLKGEKIVEVPFDVIRIAIPLLIYFVVMFLASFGM
                     AARLKSDYGKSTTIAFTAASNNFELAIAVAVAVFGIDSGAAFAAVIGPLVEVPVLIGL
                     VNVAFYFRRRYFAEAV"
     gene            2582..3106
                     /locus_tag="PLQ54_07540"
     CDS             2582..3106
                     /locus_tag="PLQ54_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOS93151.1"
                     /translation="MNVSRATRIQAALIGLLALGLASAAFAAIQPGSAPPDFTLNVAT
                     KGNINRPNDFRARRVILLDVDHRACAPCRKVLGVMQKLEKRHAGEGIQISPANSGGPL
                     AERVVPKDTKDPRISFPVLLDRDYAVAGSCGVETIPSIVLGDTKGVARLVHIGYQDDL
                     EVRLTKLIDQYRPK"
     gene            3115..>3388
                     /locus_tag="PLQ54_07545"
     CDS             3115..>3388
                     /locus_tag="PLQ54_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93152.1"
                     /translation="MGDRMRILFLCTGNSCRSQMAEGWARHLKVDTIEPYSAGLEAHG
                     LNPLAVRAMAEVGVDISGQHSKTIADLPTREFDVVVSDIRMPGADGM"
BASE COUNT          552 a         1167 c         1089 g          580 t
ORIGIN      
        1 atcgtgccca tctcgtcggc gtgatggcac ggcgtgcggt ggatgcggcg gccgccctac
       61 ggccctctcc acgccttcag ccgcacacac gaatggatgt gcagcggccg cacggtgaag
      121 gatagccgtc cgtcacgcca gtccgcgtgc aggggctcat gcccgggagc cggggtcatc
      181 gactcggcag agcgccacac cggatggaga tgccagacgg cgctagcgtc gggatgccgt
      241 agacactccg tcccgacccg gcctctgaat gtggtgacgc cactgtgtcc tttggcgtag
      301 cggaggatga acttcggccg aaccggacgg gagcgcgtcc accgccgttg gcaggtgtct
      361 gcccgcgcgg cggcgcattc ccgcagcacc acaacgtcgg cacctccgac gcctggaggg
      421 ggctacgcca tgtgtgaccg gtgtgagcag gaccacggcg gcgacgtggg ttgcgacccc
      481 aactgcacag cacgatgcgc accgccgggc caggttgacc gacgccggtt catcttggcg
      541 acctcccgtg tcgccctggc cgcggcggtc ggcctcagcg gcgtgcgcga tctggcgtgg
      601 ggccaacagg cactgcagac caccaagaac gccctcggca tggagctgac gctcatcgcg
      661 cccggcacgt tccgcatggg cagcgaggac aacgacgacg agaagccccc gcatgatgtg
      721 accatcagca agcccttctg gctcgcagtg tgcgagacca gcaacgcgga ctatcgccgc
      781 ttcgtcgagg ccgccggcca cggcgagccc ggcgtcgccg tcccctcgaa gaagggcatc
      841 gtgccctgga agaaccccat gtaccgggcc gacgagcagc ccgtggtctg tgtgacccac
      901 gatgacgccg tcgccttctg tgcatggctg agcgagaccg agggtgccac ctaccgactg
      961 cctaccgagg ccgagtggga gtacgcctac cgtggtggca caaccagccg cttctattgg
     1021 ggcgacgagc cggccgggag agaggccgca tacttctcga agccttggcc cgaagagacc
     1081 cagcaggcgc cgggctacca ggagatccct tggggcctgc tcagtgtcct ccccgtgtcg
     1141 tgcgaggaac ggaacgccca cgggctctgc cacatggcgg ggaacgtctg ggagtgggtc
     1201 gccgactggt atggccccta cgccgcagac gcccagaccg acccgatagg ccccgccgag
     1261 ggcacccagc gggtaacgcg cgggggctcg cggttccacg cgagccgcgt cgcgactgcc
     1321 tccgtgcgcc ggccgatgga cccggacacc tgctgccaca accgcggctt ccgcgtcgtg
     1381 cgcgaggcgt agcttctcca ggaccatccc ccgtggagcc ctgcggggcc cgacagcgac
     1441 ttgagcccca ttcgctttat cgtataatag cgatgatgcg gttgtgcgat gatgtctcgt
     1501 ggaggtcgcg ccatgccttc agttgccaag cagctgtccg tgctcgaccg gtacctcacc
     1561 gcgtggatct tcgccgccat ggtcctcggc gtggcgctcg ggtgggctgc ccagtcgacc
     1621 gtcgaggcct tcaaccaggc gacgcaggtt ggcactacct cgctgcccat cgccgtcggc
     1681 ctcatcctga tgatgtaccc gcccctggcg aaggtccgct acgaggccat gggcccggtg
     1741 ttccgcgacg tcaaggtgct ggccctgtcg ctcatccaga actgggtcat cggcccggtg
     1801 ctgatgttcg cactggcggt cctgctgctc cgcgacaagc ccgactacat ggtcggcctc
     1861 atcatgatcg gcctggcgcg gtgcatcgcc atggtcatcg tctggaacga cctggcgcag
     1921 ggcgaccgcg agtactgcgc cgcgctcgtc gccttcaact ccgtgttcca ggttgtcttc
     1981 tactcaatct acgcctacgt cttcgtcaca gtcctgcccc cgttggtcgg actggccggg
     2041 gcgcgggttg aggtcaccat cgggcagatc gccaagagcg tgttcatcta cctcggcata
     2101 ccgttcctgg cgggcatcat cacgcgcttc tcgctgctga ggctcaaggg actcgactgg
     2161 tacgagcacc gattcgtccc ccgcatcagc cccatcaccc tggttgccct gctgttcacg
     2221 atcgtggtca tgttctcgct caaaggcgag aagatcgtcg aggtgccatt cgacgtgatc
     2281 cgcatcgcca tcccactgct catctacttc gtggtgatgt tcctggcgtc gttcgggatg
     2341 gccgcgcggc tcaagtccga ctacggcaag tccaccacca ttgccttcac cgcggccagc
     2401 aacaacttcg agctcgccat cgccgtcgcg gtcgcggtat tcggcatcga ttcgggggcc
     2461 gcgttcgccg ccgtcatcgg gccgctggtc gaggtgcccg tgctgatcgg gctggtcaac
     2521 gtcgccttct acttccggcg ccgctacttc gcggaagccg tctagctcag gggggtatcc
     2581 cgtgaacgtc tcacgcgcca cgcggatcca agccgcgctc atcggtctgc tggccctcgg
     2641 gctcgcctca gccgcgtttg cggccatcca gccgggttct gcgccccccg acttcacgct
     2701 caatgttgcg acgaagggca acatcaacag gccaaacgac ttccgagcca ggagggtcat
     2761 actcctcgat gtcgaccacc gagcatgcgc tccatgccgc aaggtcctcg gcgtgatgca
     2821 gaagctcgag aagcggcatg cgggcgaggg catccagatc tcccccgcaa acagcggggg
     2881 acccttggcc gagcgggtcg tgcccaagga caccaaggac ccgaggatct ccttcccagt
     2941 cctgctggat cgcgactacg cggtggccgg atcctgtggc gtcgagacga tcccatcgat
     3001 cgtcctgggc gatacgaaag gcgtggcgcg cctggttcac attggctacc aggacgacct
     3061 cgaagtgagg ctgacgaagc tcatcgacca gtaccggccg aagtaggggg tagcatgggc
     3121 gaccgcatgc gcatcctgtt cctctgcacc ggcaactcgt gccgaagcca gatggccgaa
     3181 ggctgggcgc gccatctcaa ggtggacacg atcgagccat actcggccgg cctcgaggcc
     3241 catggtctca acccgctggc cgtcagggcc atggccgagg tgggcgtcga catctccggc
     3301 cagcactcca agacgatcgc cgatctaccg accagggagt tcgacgtggt cgtcagcgac
     3361 atccggatgc cgggcgccga cggtatga
//
LOCUS       DAOSPL010000344         3385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28126_length_3385_cov_2.575676, whole genome shotgun sequence.
ACCESSION   DAOSPL010000344 DAOSPL010000000
VERSION     DAOSPL010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28126_length_3385_cov_2.575676"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="PLQ54_07550"
     CDS             <1..1234
                     /locus_tag="PLQ54_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93153.1"
                     /translation="DWARAHDPGADGRRDNRLSLSSRLARFRWDGTPAVAVRAGVAPK
                     VRLDAVVDALAWYFDVATPTDDTLHLLLGAEDGADPAALSDQLGAIGTLATSIVDGPQ
                     IRVWLLGPNGEPQHRPVRAATFDSSVVARWTEWLLTEARRPVSGLARHLVQTVDTPAF
                     ALYPKLSSKDESRPWQMRLDGLEIGRIGAESATLALATSDPTKPGEPRDTWRDVVGTE
                     PRTVGAADLGSLRALIAELVGRWSDPEHPTAVLRHGQAEHALEAHLLTGRTRLTSATA
                     GVLRPAGLIVSGVVRSAQFPTLWGGPPRARYLDALLADDAGRPWAIELKDQDAGGGMG
                     AYLRHGIGQAVLYRHFISAATALDPYFAELGLGRAGCKAALAFPTAAPSAAVKIDGHR
                     RVAAAFGVEVIEFPRPGQ"
     gene            complement(1287..1496)
                     /locus_tag="PLQ54_07555"
     CDS             complement(1287..1496)
                     /locus_tag="PLQ54_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOS93154.1"
                     /translation="MGRRLDVDLLVGSAEIAHRLSLSHQNVVHVWQERHPDFPQPVVR
                     LRAAKVWYWPDVEAWAQATGRLPGP"
     gene            1612..2073
                     /locus_tag="PLQ54_07560"
     CDS             1612..2073
                     /locus_tag="PLQ54_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93155.1"
                     /translation="MSITPLGAGLRKVEALVAALEPGHRAVAEVLLEYQHEIELVQPH
                     AFGILFEPAGAFSTLQQLRIRPDFVLIGPFGAAAIEVDGTSHNGRYASDRSRDRLILN
                     SGVQTHRIPIEETRDRSDLVEVLRHPIWQVCEVDVRRLDLWWDNAPGRGAA"
     gene            complement(2103..>3385)
                     /locus_tag="PLQ54_07565"
     CDS             complement(2103..>3385)
                     /locus_tag="PLQ54_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93156.1"
                     /translation="IEDFTVGDKATLPLRPLARASTGAHLNLWERDDPFYAPGIFEGS
                     GPWWCHWKIEPSPPGDPVGTRKVPDFSTDTTLMRRMAGLLAAQRVVAALAGWHQLSSK
                     QLSAIVGYPFTKLSRILKPLFELGIVERGHYPKEQVFLRTDYLYRLRIDKPLRTFLGL
                     LDDETRYSVTLGIAPVPAGAAVRHDLLAAEFAIRALELIPGVVAVFPESVSSPSLLLR
                     HLGRKETWRGDLTLLRSDGLRIVIEITRARHPGEIEFKMRRWGRLLMTHNWHETGLMV
                     VFVNAAVPNSGLESIEGEHRMQARLLRDTYTRALSQECLSSDEVRAMWRHLARARAQI
                     HLASWMDWFPAPGLVSGSFPSLPTVFNAGDGRWRRRGLSDPNGFWDFDPWPRTARIVN
                     AHLANYQKAYSCPRFRWQPAANRSLHPVVSRDKA"
BASE COUNT          505 a         1154 c         1194 g          532 t
ORIGIN      
        1 cgactgggcc cgggcccacg accccggtgc cgacggccga cgggacaacc ggctctcgct
       61 gtcctcgcgc ctggcccgct tccgctggga cgggacaccc gccgttgccg tgcgggccgg
      121 cgtggctccc aaggtccgac tcgacgcggt cgtcgacgcg ctcgcctggt acttcgatgt
      181 cgccacaccg accgatgaca cgctccatct gctcctgggc gccgaggacg gcgccgaccc
      241 cgcagcgctg agcgaccagc tcggcgccat cggcactctc gccacctcga tcgtcgacgg
      301 accgcagatc cgggtgtggc tgctcggccc gaacggcgag ccgcagcacc gaccggtgag
      361 ggcggcgaca ttcgactcgt cggtggttgc gcgctggacc gagtggctgc tcaccgaggc
      421 caggcgcccg gtgagcggcc tcgcccgcca cctcgtccag accgtcgaca ctccggcctt
      481 cgccctgtac cccaagctgt catcgaagga cgagagccgg ccgtggcaga tgcgtctcga
      541 cggcctcgag atcggccgca tcggcgccga gagcgccacg ctggcgctgg cgacgagcga
      601 ccccaccaag cccggcgagc cccgggacac ctggcgggac gtcgtcggca ccgagcctcg
      661 gaccgtcggt gcggccgatc tcggctccct gagggccctt atcgctgagc tggtcggccg
      721 ctggtccgat cccgagcacc cgaccgccgt gctgcgccac ggccaagccg aacacgccct
      781 cgaggcgcac ctcctcaccg gtcgcacccg gctcaccagc gccacggccg gcgtgctcag
      841 gccagccggc ctgatcgtgt cgggcgtggt gcgaagcgcc cagttcccga ccctgtgggg
      901 cggaccacca cgggcccgct acctggacgc cctcctggct gatgacgcag ggcggccctg
      961 ggccatcgag ctgaaggacc aggacgccgg cggcggcatg ggcgcttatc tccgccacgg
     1021 catcggccaa gccgtgctct accggcattt catcagcgcc gccacggccc tcgaccccta
     1081 cttcgccgag ctcggtcttg ggcgggccgg gtgcaaggcg gccttggcct tcccgaccgc
     1141 tgcgccctct gctgctgtga agatcgatgg tcaccgccgg gtcgcggccg ccttcggcgt
     1201 tgaggtcatc gagttccccc gccccggcca gtagagcggt ggcgcgaagg cggtagccgc
     1261 cggacgggag ccgcaggtgg gtgtgcctac gggccgggga gtcggcccgt ggcttgggcc
     1321 caggcttcga catccggcca gtaccagacc ttggcggccc tgaggcgaac gacgggctgg
     1381 gggaagtccg ggtggcgctc ctgccagacg tggacgacgt tctggtggct gagcgagagg
     1441 cgatgcgcga tctcggcgga accgacgagg agatccacgt cgagtcgtcg gcccacgacg
     1501 gcggaaccta cgcgtccggc cagcggcgtg ccagatgttt gatcgaggat tcgatcaccc
     1561 ggtactgtta gagaacgtaa acagtagagg tgggggagaa ggagtcggta catgtccatc
     1621 accccgctgg gcgcgggtct tcggaaggtc gaggcgctcg tggccgcgct cgagccgggc
     1681 caccgtgccg ttgccgaggt gctgctcgaa taccagcacg agatcgagct ggtccagccg
     1741 cacgcgttcg gcatcctgtt cgaaccggcc ggcgccttct ccaccttgca gcaactacgg
     1801 atccgacccg acttcgttct catcggcccc ttcggtgctg cggccatcga ggtcgacggg
     1861 accagccaca acggccgcta cgcgtcggac cgttcccgcg accggctcat cttgaacagc
     1921 ggcgtgcaga cccatcgcat ccccatcgag gagacccgag accgctccga cctggtcgag
     1981 gtgctccgtc acccgatctg gcaggtgtgc gaggtcgatg tccggcgcct cgatctgtgg
     2041 tgggacaatg ctcccggccg cggcgccgca taggcggcag ccggggctga ctcgtgtgtg
     2101 gttcaggcct tgtctctcga gaccacgggg tggaggctcc ggttcgcggc cggttgccac
     2161 ctgaagcgcg ggcatgaata cgccttctgg tagttggcca ggtgagcatt cacaatccgt
     2221 gcggtcctgg gccaaggatc gaagtcccag aagccgttcg ggtcgctgag ccctcgacgc
     2281 cgccatcggc cgtcgccggc gttgaacact gtggggaggc tggggaagga gccgctgacc
     2341 aggccggggg ccgggaacca gtccatccag gaggcgaggt ggatctgggc tcgggcgcgc
     2401 gcgaggtgtc gccacatggc gcgaacctcg tcgctgctga ggcactcctg gctgagggcg
     2461 cgggtgtagg tgtcgcgcag taggcgggcc tgcatccggt gttcgccctc gatggattcg
     2521 agaccgctgt tgggaaccgc cgcgttgacg aacaccacca tgaggccggt ctcgtgccag
     2581 ttatgggtca tcagcagccg cccccagcgg cgcatcttga actcgatttc gccggggtgt
     2641 cgggcccggg tgatctcgat gacgatgcgc agcccgtcgg agcgcagcag tgtgagatcg
     2701 ccacgccatg tctccttgcg cccgaggtgc ctgagcagca gggaagggct ggacacggat
     2761 tcggggaaca cggcgaccac cccggggatc agctcaagcg ctcggatggc gaactcggcg
     2821 gcgagcaggt cgtggcgcac agcggcgccg gcggggacgg gagcgattcc gagggtgacc
     2881 gagtaccggg tctcgtcgtc cagtagcccc aggaacgtgc gcagcggctt gtcgatccgg
     2941 agtcggtata ggtagtccgt tcgcaggaac acctgctctt tggggtagtg gcctcgttca
     3001 acgatgccga gctcgaagag cggcttgagg atcctcgaca gcttcgtgaa cgggtagccg
     3061 acgatggcgg agagctgctt gctcgacagc tggtgccatc cggccagcgc cgccaccaca
     3121 cgctgtgcgg ccaacagccc ggccatcctc cgcatcagcg tggtgtcggt cgagaagtca
     3181 gggacctttc tggtgccgac cgggtcgcct ggtgggctcg gctcgatctt ccagtggcac
     3241 caccagggac cgctgccttc gaagatccct ggtgcgtaga aggggtcgtc gcgttcccac
     3301 aggttcaggt gggccccggt gctggcgcgg gcaagcgggc gaagcggcag cgtcgctttg
     3361 tccccgaccg tgaagtcctc gatga
//
LOCUS       DAOSPL010000345         3382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28151_length_3382_cov_2.956718, whole genome shotgun sequence.
ACCESSION   DAOSPL010000345 DAOSPL010000000
VERSION     DAOSPL010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28151_length_3382_cov_2.956718"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="PLQ54_07570"
     CDS             complement(<1..761)
                     /locus_tag="PLQ54_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOS93157.1"
                     /translation="MLALVRIELSKLYRRRGTYVSFACLAALVLLVAVPISCYKEKVD
                     HSLRDELSSGMGDDWVVGGKVLAGPFVPRVMLFWPMRKLVVNILLALFAAMAGGSAIA
                     GEYSAGTLRTILTRPPARWKVFVAKFVVMAIYAASLAIFFGLFAFIVGYALFGRGALV
                     FADMPADMGSGPRPMGAPIIQEGAAVQMLGQAYLLMGVSIIAAMSLALFVSSFTSHPL
                     TAAGAALGILLLSTIVGTLGSAPKEIEFLAFFRTIR"
     gene            complement(739..1671)
                     /locus_tag="PLQ54_07575"
     CDS             complement(739..1671)
                     /locus_tag="PLQ54_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS93158.1"
                     /translation="MEPVIECQGLTKRYGSLLAVDHVDLAVGRGEVFGFLGPNGAGKS
                     TTIRMLLGLIRPDAGRALLFGQSVAAGQRRVLKRVGALVESPALYDYLSGRKNLEILS
                     DMSGGCPRKRIDEVLELVGLSGRQKDRVGTYSHGMRQRLGLAQALLPKPELLVLDEPA
                     TGLDPEGIVEIRNLLRYLGESEQMTIFVSSHLLHEVQATCTDVGLLSRGRLVTRGRVA
                     DLLRQGHLEATIGIDDAARGREVLGSLPFVLAVEGEDSALRVRVEAERLADANRALVA
                     AGLAVSALVPRQGNLEELYMSVMTEAGHACSSAD"
     gene            complement(1786..2757)
                     /locus_tag="PLQ54_07580"
     CDS             complement(1786..2757)
                     /locus_tag="PLQ54_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS93159.1"
                     /translation="MPEVIRAAAIGAGSMANAVHYPSLASFDDVSLVGICDLDVSRLN
                     ATADRYGVEGRYTNYQAMIEDLAPDAVYAIGQPHILFDVWMWCLSHGLNLYIEKPMGL
                     SLHQARALAHVAERNGCITQVSFQRRTCPMVVQLREACLKRGPITHGVCQFYKWAPEP
                     FLGARDHMMDDGVHAIDTLRWMCGGEVADIQCVTRRVGTPDINFIVATLGFDNGATGV
                     LMNSWTSGRRVFRVEMHAPGICAEAEHEGTGRLFADGDAAGTEYDTREVAGSDEFHVY
                     AGFSAKHREFIDALKTGTQPGSHFGDALKTMEVAERILARSLLGREC"
     gene            complement(2802..>3382)
                     /locus_tag="PLQ54_07585"
     CDS             complement(2802..>3382)
                     /locus_tag="PLQ54_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOS93160.1"
                     /translation="GPTDEATIAACGEAGVSIIRICEAIDDDGYLATEARLQRKYAAL
                     VPLLDKHRVTLGIQNHSGNHVASAMGLRHLIEQFDPRHIAAVWDPAHTAVSGEEPHLA
                     IDILASHLCMVNLKNVFWQRTNGPEAPVAEWSMYWTTGRHGLASWPRVVELLRARGYA
                     GPVCLCAEYSSGHDVNRLIAQDIAFAKSIFEG"
BASE COUNT          611 a         1135 c         1101 g          535 t
ORIGIN      
        1 cggatggtcc tgaagaaggc caggaactcg atctccttgg gcgcactacc gagggtgccg
       61 acgatcgtgc tgagcaggag gatcccaagg gcggcgccgg cggcagtcag ggggtgcgat
      121 gtgaaggacg agacgaacag cgccagtgac atggcggcaa tgatggagac tcccatcagc
      181 agataggcct gccccagcat ctgcacggcg gcaccctcct ggatgatggg tgcgcccatc
      241 ggccgaggcc ccgaacccat gtctgccggc atgtcggcaa agacgagggc tcctcgcccg
      301 aacagggcat agcccacgat gaaggcgaac agcccgaaga agatggccag ggacgccgcg
      361 tagatggcca tcaccacaaa cttggcgacg aacaccttcc atcgcgccgg aggacgggtc
      421 aggatcgtcc ggagcgttcc ggctgagtac tcccccgcaa tggcgctgcc gccggccatg
      481 gcggcgaaca gtgccaggag gatgttcacc acgagcttcc gcatgggcca aaacagcatg
      541 acgcgcggca cgaacggccc ggccaggacc ttgcccccga ccacccagtc gtcgcccatg
      601 ccgcttgaga gctcgtcgcg cagactgtgg tcgaccttct ccttgtagca cgagatcgga
      661 actgccacca gcagcacgag cgcggccagg cacgcgaagc tgacataggt cccgcgtcgg
      721 cgatagagct tgctgagctc aatccgcact agagcaagca tggcccgctt ccgtcatcac
      781 agacatgtag agctcctcga ggttgccctg gcgaggtacc agggccgaca cggccaggcc
      841 ggccgccact agcgcccggt tcgcgtctgc cagccgctcg gcctcgacgc gtacgcgcag
      901 cgccgagtct tcaccctcga ccgcgaggac gaacggcagc gagccgagca cctcgcggcc
      961 gcgggccgcg tcgtcgatgc cgatcgtcgc ctccaggtgt ccctgccgca gcaggtcggc
     1021 cacgcgaccc cgagtcacca gtcgcccgcg ggacagcagc cccacatcgg tgcatgtcgc
     1081 ctggacctcg tggagcaggt ggctcgacac gaagatcgtc atctgctcgg actctcccaa
     1141 gtaccgtaga aggttgcgga tctcgacgat gccctcaggg tccagccccg ttgcgggctc
     1201 gtcgagtacc agaagctccg gcttgggcag cagcgcctgg gccagtccca ggcgctgcct
     1261 catgccatgg gagtaggtcc cgacgcggtc cttctggcgg cccgacaggc ccacgagctc
     1321 gagcacttcg tcgattcgct tgcgcgggca gccgcccgac atgtcggaga ggatctcgag
     1381 gttcttccgg ccggacaggt agtcgtacag ggccggagac tccaccagag cccccacacg
     1441 cttgagtacc cgcctctggc cggcggccac cgactgcccg aacagcagtg cacgaccggc
     1501 atcggggcga atcaggccga ggagcatgcg gatggtggtg ctcttgcccg cgccgtttgg
     1561 gcccaggaac ccaaacacct cgccgcgacc gacggccagg tccacgtggt cgacggccag
     1621 caagctgccg tagcgcttgg tgagcccctg gcactcgatg accggttcca tggcactccc
     1681 gggaacgctg acgcggcagg gacaggagtc acccccgccg cgctgtgtgg atgtcagtag
     1741 agacgcactg gcgccgggcc ggttgcggct tcctcgatcc gggatctagc actcgcgtcc
     1801 gagcagcgac cgcgccagga tccgctccgc gacctccatg gtcttcaggg catcgccgaa
     1861 atgcgaaccg ggctgggtgc ccgtcttcag tgcgtcgatg aactcacggt gcttggcgga
     1921 gaagccggcg tagacgtgaa actcatcgct tcccgctacc tcacgcgtat catactcggt
     1981 cccggcggca tcgccgtcgg cgaacagccg gcctgtgccc tcgtgctcgg cctccgcaca
     2041 gatgcccggg gcgtgcatct cgacgcgaaa cacgcgccgg ccgctcgtcc agctgttcat
     2101 cagaacaccg gtggcgccgt tgtcgaaccc gagtgttgcc acgatgaagt tgatgtcggg
     2161 ggtacccaca cggcgcgtga cacactgaat gtcggccact tcgccgccgc acatccaccg
     2221 gagtgtatcg atggcgtgca caccgtcgtc catcatgtga tctcgggcac cgaggaacgg
     2281 ctcgggggcc cacttgtaga actggcagac cccatgggtg attggtccgc gcttgaggca
     2341 tgcctccctc agttgcacca ccatgggaca cgtgcggcgc tggaaactca cttgagtgat
     2401 gcatccatta cgctccgcga cgtgggccag agcccgggct tggtgtaatg acagacccat
     2461 gggcttctcg atgtacaagt tcaggccatg cgagagacac cacatccaga catcgaacag
     2521 gatgtggggt tgcccgatgg catagacggc atcgggagcc agatcctcga tcatggcctg
     2581 gtagttcgtg tagcgcccct cgacaccgta gcgatcggcc gtggcattga gccggctcac
     2641 atccaggtcg cagatgccca caagggagac gtcatcgaac gacgcgagtg acgggtagtg
     2701 gacggcattg gccatgctgc ccgcaccaat ggcggccgcg cggatgactt cgggcaccga
     2761 tcgagcactc cctcactggc cggaagggtg gggcgcgggc ctcatccctc gaagatcgac
     2821 ttggcgaagg cgatgtcctg cgcgatgagc cggttcacgt cgtggccgct gctatactcg
     2881 gcacacaggc acaccggccc ggcataccct cgggcccgca ggagctcgac cacgcgaggc
     2941 catgaggcca gaccgtgccg gcccgtggtc cagtacatac tccattccgc caccggggcc
     3001 tcggggccat tcgtgcgttg ccagaacacg ttcttgaggt tcaccatgca caggtgcgac
     3061 gcaaggatgt cgatggccag gtgcggctcc tcgccactga ccgcggtgtg ggcggggtcc
     3121 cacaccgcgg cgatgtggcg cgggtcgaac tgctcgatga ggtgccggag tcccatggcg
     3181 ctcgccacgt ggttcccgga atggttctgg atgcccagcg tcacccgatg cttgtccagg
     3241 agcggcacca aggcggcata cttgcgctgt agccgggcct cggtcgcgag gtagccatca
     3301 tcatcgatgg cttcgcagat gcgaatgatg gagacgccgg cctcaccgca cgcggcgatg
     3361 gtggcctcat ccgtcggtcc gg
//
LOCUS       DAOSPL010000346         3377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28213_length_3377_cov_3.469898, whole genome shotgun sequence.
ACCESSION   DAOSPL010000346 DAOSPL010000000
VERSION     DAOSPL010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28213_length_3377_cov_3.469898"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="PLQ54_07590"
     CDS             <1..1294
                     /locus_tag="PLQ54_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93161.1"
                     /translation="EKTMYQGSAEISRLATSVAAAANSVAEVTRGLRQFTTDGTLAAD
                     AKKGVSALAKTGEHLTRVAETLDDLLSSEGGKSRMQTVLESAERTATELERITRDVSA
                     ITGDTGNRAHLKSMLEDAAKASANLAAMSDDVRQMFGREGLKDEVARIMASVESASAK
                     LDRVAGSLDELTSDDRLKADVRATASGLASAAGRLDRMMDEELESGVREVSRSLHQLT
                     EDLTVTAREARRLLVDSGTSGNMEQAMRSLREAADLILDMARDVDQATGPSQSGGLGQ
                     FTRDAVGIMPDVKEALKVAKKVEHSTLDIHEDLLYSVRDRRFSTYFDVDWMGRNDTGA
                     VLGMGDVGRTNWVRAQLSHRAGEFTGRFGLNHTTAGVGVDWRPSRRSVLSLDVYNGNA
                     PDLDLRAVQMIGDHVRLVGGADQLGRSPNWLLGVGYRF"
     gene            complement(1401..>3377)
                     /locus_tag="PLQ54_07595"
     CDS             complement(1401..>3377)
                     /locus_tag="PLQ54_07595"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOS93162.1"
                     /translation="ATVLSCVGRHHELLLGTATAPSPPEEVRAATEAHVLFGGGYCVN
                     HSVRDYGLVVCRGFRALAEEIDTALAACRIEDPDDIQKLVFYRGASAVAHAAEGLGRR
                     YAEVARAEAATCADPARRAELERMAEACERVPAEPARTLFEAVQAIWLAHAITCCEDH
                     INANSIGRLDQILQPYYEADVTAGRMDREGAIQLIELLWLKLYRDYDVQQVVLSGQRP
                     DGTDATNDMTYICLEATRRLGLVRCLSVRLHKGSPRKLLEEATDLLSQGGGIPFFFND
                     DVIVPALCDKGIPLEDARDYAVIGCVEITIPGRTNPHAVSHNTNLAKCLELALHDGFD
                     PATGRQVGPHTGLLESFGSMREVFEAYKRQVEHFTRIGAFLSNAGEQQQESTFPLPYE
                     SILTADCIARGRDITAGGARYNYHSCSAIGIPNVADSLAALDRLVFGTRTIGAEELRR
                     ALLADFEGYEPLRQMLLHSAPKYGNDREEVDALAAEVSAHFCETLAGHRARFDGRFHA
                     HLFSFLWHVDPFGRSTGALPDGRKAGQPLAYSLSPTQGRDEQGVTAVLNSLAAIPHDM
                     AAASSSAIIELAPSFFAGEGRNRFVDLLQSAIARGIGQMQFNVVTAERLKLAQEDPEN
                     YGNIVVRVSGFSQRFASIGRELQDHIIARVKHDH"
BASE COUNT          582 a         1085 c         1181 g          529 t
ORIGIN      
        1 cgagaagacc atgtaccagg ggagtgccga gatctcgagg ctggccacga gtgtggccgc
       61 cgcggcgaac tcggttgccg aggtgacccg cggcctccgg cagttcacga ccgacgggac
      121 actcgccgcc gacgccaaga agggtgtctc cgccttggcc aagaccggtg agcatctgac
      181 tcgcgtggcc gagacgcttg acgacctcct gtcgagcgaa ggcggcaagt cgcgaatgca
      241 gacggtgttg gagtcggccg aacggaccgc cacggagctc gagcgcatca cgcgcgacgt
      301 cagtgccatc acgggcgaca ccggcaaccg cgcccacctc aagtcgatgc tcgaggatgc
      361 ggccaaggcg tccgccaacc tggcggccat gagcgatgac gttcgccaga tgttcgggcg
      421 ggaaggactc aaggacgagg tggcccgcat catggcctcg gtggaatcgg cctcggccaa
      481 actggatcga gtcgcgggtt cactcgatga gctgaccagc gacgaccggc tgaaggccga
      541 cgtcagggcc acggcctcgg gattggcgag cgccgccggc cggctcgacc ggatgatgga
      601 cgaggagctg gagtcgggtg tgcgcgaggt atcgaggagc ctgcaccagc tcaccgagga
      661 cctgacggtg accgcccgag aggcccggcg cctgttggtg gactcgggca cgagcgggaa
      721 tatggagcag gccatgagaa gcctgcgtga ggcagcggac ctgatcctcg acatggcacg
      781 agacgtcgac caagccacgg gtccgtcgca gagcggtggc ctggggcagt tcactcgcga
      841 cgcagtcggg atcatgccgg acgtcaaaga ggcgctcaag gttgccaaga aggtcgagca
      901 ctcgacgctc gacatccacg aggacctgct ctattcggtc cgcgaccgac gcttcagcac
      961 gtacttcgat gtcgactgga tgggccgcaa cgataccggt gcggtactcg gcatgggcga
     1021 cgtcggccgc accaactggg tgagggcgca gctcagccac cgcgccggcg agttcacggg
     1081 gcggttcggc ctcaaccaca ccaccgcagg ggtgggcgtc gactggcggc cgtcgcggcg
     1141 gtcggtgctg agcctcgacg tctacaacgg caacgcgccc gacctggacc tgcgggccgt
     1201 ccagatgatc ggcgaccacg tccgcctggt gggcggtgcc gaccagctgg gccgtagccc
     1261 gaactggctg ctcggggtcg ggtaccggtt ctgagacgcg gcgtctgccc gccatggacg
     1321 gctggggcgc cgacgcagtg tccgttcggc gagaggcgca gtccccgggc agagcgcgac
     1381 cccttcgggt ctcggcctcc tcagtgatcg tgtttgacgc gggcgatgat gtgatcctgc
     1441 agctccctgc cgatcgaggc gaaccgctgg ctgaatccgg agacgcggac cacgatattg
     1501 ccatagttct cgggatcttc ctgggcgagc ttcagcctct cggcagtgac cacgttgaac
     1561 tgcatctgcc cgatccctcg tgcgatggct gactgcagca gatcgacgaa ccgattccgt
     1621 ccttcgccgg cgaagaatga cggcgcgagc tcgatgatgg ccgagctcga cgcggcggcc
     1681 atgtcatgcg gaatggcggc gaggctgttg agcacggcgg tgacgccttg ctcatcccgc
     1741 ccctgggtgg gtgaaaggct gtaggccagc ggctgccccg ccttccggcc atcggggagc
     1801 gccccggtgc tccgcccgaa gggatcgaca tgccacagga aggagaacag gtgtgcgtgg
     1861 aaccggccgt caaaccgcgc gcggtggccg gccagggtct cgcagaagtg ggccgagacc
     1921 tcggccgcca gcgcatccac ttcctcccgg tcattcccgt acttgggggc cgagtggagc
     1981 agcatctgcc gcagaggttc gtagccctcg aagtcggcca gcagcgcccg tcgtagctct
     2041 tccgccccga tcgtccgtgt gccaaacacc aagcggtcca gcgccgccag cgagtccgcc
     2101 acattcggga tcccgatggc cgagcacgaa tggtagttgt accgtgcgcc tccggccgtg
     2161 atgtccctcc cacgagcgat gcagtcagcg gtcaggatgg actcatacgg gagcggaaaa
     2221 gtggactcct gttgctgttc ccccgcgttg gacaggaacg cgccgatgcg ggtgaagtgc
     2281 tcgacctgac gcttgtaggc ctcaaacacc tcgcgcatgg agccgaagga ctcgagaaga
     2341 cccgtgtgcg gtccgacttg ccgaccggtg gccgggtcga agccatcgtg cagggcaagt
     2401 tcgagacact tggccaagtt ggtgttgtgg ctcaccgcgt gggggttggt tcgaccggga
     2461 atcgtgatct caacacaccc gatgacggcg tagtcgcgag cgtcctcgag ggggatcccc
     2521 ttgtcgcaga gggccggtac gatgacgtcg tcgttgaaga agaacggaat gccgcctccc
     2581 tggctcagca ggtcggtggc ctcctcgagc agcttccgcg gcgaaccctt gtgcaggcgc
     2641 accgacaggc atcgcaccaa gcccaggcgg cgggtggcct ccaggcagat gtaggtcatg
     2701 tcgttggtgg cgtcggtgcc atcgggacgc tgaccgctca gcacgacctg ctgcacgtca
     2761 tagtcgcggt acagcttcag ccatagcagc tcaatgagct ggatggcacc ctcgcggtcc
     2821 atccggcctg cggtgacatc ggcctcatag tacggctgga ggatctgatc gagccggccg
     2881 atggagttgg cgttgatgtg gtcctcgcag caggtgatgg cgtgggccaa ccagatggcc
     2941 tggacggcct cgaagagcgt gcgggccggc tccgccggca cgcgctcgca tgcttccgcc
     3001 attcgttcga gttcggcgcg acgggcgggg tcggcacacg tcgccgcctc ggcccgggcg
     3061 acctcggcat agcgcctgcc caggccctcg gccgcgtgtg ccaccgccga ggcaccacgg
     3121 tagaagacca gcttctggat gtcgtcgggg tcctcgatcc ggcacgcggc cagggcggtg
     3181 tcgatctcct ccgccagggc gcggaagccc ctgcagacca cgaggccata gtcgcgaaca
     3241 ctatgattca cgcaatagcc cccaccgaag agaacgtggg cctcggtcgc cgcgcggacc
     3301 tcttcgggcg gtgagggagc agtcgccgtg ccgaggagaa gctcgtggtg acggcccaca
     3361 cacgagagga ctgtcgc
//
LOCUS       DAOSPL010000347         3377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28215_length_3377_cov_3.251656, whole genome shotgun sequence.
ACCESSION   DAOSPL010000347 DAOSPL010000000
VERSION     DAOSPL010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28215_length_3377_cov_3.251656"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLQ54_07600"
     CDS             complement(<1..188)
                     /locus_tag="PLQ54_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93163.1"
                     /translation="MTMVPVARALFLAAPLLAAGCGPYASGGPTIVSLTPTATSVRVG
                     GTIDVTAEVSSPSLGTLT"
     gene            335..3175
                     /locus_tag="PLQ54_07605"
     CDS             335..3175
                     /locus_tag="PLQ54_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HOS93164.1"
                     /translation="MRCLILVFVASIGLGCRATAAPEATVDGASLVANPNATFEVQVA
                     GKLAVRTGLAGWHGEWTYFTEASNEGAEERQDGDARVVTTRIPKDEGVSPLAVTARIA
                     SVPDGVRIEYEFAKTGELVLRRGVLGTLYLRHDPMRGRRIFLEGTRCARIPAAVSGST
                     RRMGVEVANGLFLTAEFDRPFRVELGEAGGDGCTVRMELTPADFPVGETQRASVTYRL
                     ERIAGALAGEVTGGAAPLAIRAIHLTQATVPRFGRMEATVDLSAMYANPFDPDQVTLD
                     ASIRTPSGAKVIVPGFYLIDYDRRVEDGAEVLTAQSGRWCIRYTPAEMGRHELTAMLH
                     DATGTVISDPVGFDVGPSEAQGFVRVSKDDPHYLVFDTGTTFFPIGHNLPTYHTSGQL
                     ADDALRRMAAAGENYNRWWMYSGGLGLEWEHAVGWYRMDEAYRLDHALDLAEELGLYY
                     MLCFDTHQDFIGTNPWDRWFQNPYNKSMGGPCEKASDWFTSPEARKAYQKRLRYIVAR
                     YGYSPHVLAWEFGNEFEGWPDTPHESLVAWHREMGDYLKSIDPFHHMVTTSFWSQTGP
                     ADIWELPQMDIVQTHFYLNSDDDMAPRVAEFCKHQYRRFDKPHIFGEFGIRSHESTED
                     KDPKGWGLHNTFWPAMATFCAGAPMPWWHENYIDPLDLYFHFTSIARFTEGLDFARER
                     FQPVRIQALANADGTPLPRGDLVLRPGNRFTRSEDSRFEVQPEGTVTNAGELNGLLHG
                     QGHTDLRNPPTFVAEFPEESQFTMHIGRVSNSGHVRIWLDGALVNETDLPCGGNIGTS
                     WKYQEQWKLWESVYDTDITIPVPAGRHEIKVDNDGRDWVAVTEYRFGGLLPPGSGALV
                     AEGMRGEHTAVLWLQNPASTWKAQATGAEVAPVGPLRLTLADLPDGPCTVEFWETWKG
                     ALQETRQATVAGGRLALDLPAIASDIALKVRY"
BASE COUNT          547 a         1165 c         1156 g          509 t
ORIGIN      
        1 taggttagcg tccccaacga gggactgctg acctcggccg tgacatcgat cgtgcccccg
       61 acgcgaacgc tcgtcgccgt cggagtgagc gagacgatcg tcggcccccc gctggcatag
      121 gggccgcaac cggcagcgag cagcggcgcc gctaggaaca gggcacgtgc gaccggcacc
      181 atcgtcatgg gcccacccac ctccgacggc tggttgactg gatagttggt tgagcgaggc
      241 gagactcctc cccggggcgc cacgggatcg tgacgggagg tgaggcggcg gttcgcgagc
      301 gaaccaagtc tccagactct ggggggtggt tcgcgtgcga tgcctgatcc tggtcttcgt
      361 cgcgtcgatc ggcctgggct gtcgagcgac ggccgcgccg gaagctaccg tcgacggtgc
      421 ctcgctcgtg gcgaacccga atgccacatt cgaggtgcag gtcgccggca agctggccgt
      481 ccggacgggg ctggccggat ggcacggcga gtggacctac ttcaccgagg cgagcaacga
      541 gggcgccgag gaacggcagg atggcgatgc gcgggtcgtg accacgcgca tccccaagga
      601 cgagggcgtg tcgccgctgg ccgtgaccgc taggatcgcg tccgtgcccg atggcgtccg
      661 catcgagtac gagttcgcca agaccggcga gctggtactc cggcgcggcg tgctcggcac
      721 gctctacctg cgacacgatc ccatgcgggg ccggaggatc ttcctcgagg ggacgcgctg
      781 tgctcggatc ccggctgccg tgtctggctc gacgcggcgc atgggcgtcg aggtcgccaa
      841 tggcctcttc ctcacggcag agttcgaccg ccctttccgc gtggagcttg gcgaggccgg
      901 tggcgacgga tgcacggtcc gcatggagct cacgcccgcc gacttccccg tgggcgagac
      961 gcaacgcgct tcggtgacct accgcctcga gcgcatcgcg ggtgcgctcg ccggcgaggt
     1021 gaccgggggc gcggcgccgt tggccatcag ggccatccac ctgactcagg ccaccgtgcc
     1081 gcgttttggc cgcatggagg cgacggtcga cctctcggcg atgtacgcca acccgttcga
     1141 ccccgaccag gtgacgctcg acgcgagcat ccgcacgccc tcgggcgcca aggtcatcgt
     1201 acccggcttc tacctcatcg actacgaccg ccgcgttgag gatggcgccg aggtgctcac
     1261 cgcccagagc gggcggtggt gcatccgcta cacgccggcg gagatggggc gccatgagct
     1321 cacggccatg ctgcacgacg ccacgggcac ggtgatctcg gacccggttg gattcgatgt
     1381 ggggccgagc gaggcccaag ggttcgttcg cgtgtcgaag gatgacccgc actatctggt
     1441 cttcgacacc gggacgacct tcttccccat cggccacaat ctgccaacct atcatacctc
     1501 tggccagctc gccgacgacg ccctgcgtcg catggccgcg gcgggcgaga actataaccg
     1561 ctggtggatg tactcgggtg gcctcgggct tgagtgggag cacgccgtgg gctggtaccg
     1621 catggacgag gcctaccggc tcgaccacgc gctcgacctg gccgaggagc tcggcctgta
     1681 ctacatgctc tgcttcgaca cccaccagga tttcatcggc accaacccgt gggaccggtg
     1741 gttccagaac ccctacaaca agtcgatggg cggcccgtgc gagaaggcgt cggactggtt
     1801 cacctcgccc gaggcacgga aggcctacca gaagcgcctg cggtacatcg tggcgcgcta
     1861 cggctactcg ccccacgtgc tcgcgtggga gttcggcaac gagttcgagg gctggcccga
     1921 tacgccgcac gagtcgctcg tcgcctggca ccgcgagatg ggcgactacc tgaagtccat
     1981 cgatcccttc caccacatgg tgacaaccag cttctggagc caaacgggcc cagcggacat
     2041 ctgggagctc ccgcagatgg acatcgtcca gacgcacttc tacttgaaca gcgatgacga
     2101 tatggcgccc cgcgtggccg agttctgcaa gcaccagtac cggcggttcg acaagcccca
     2161 catcttcggc gagttcggca tccgcagcca cgagagcacc gaggacaagg atcccaaggg
     2221 ctggggactg cacaacacgt tctggccggc gatggccacc ttctgcgccg gggcgccgat
     2281 gccgtggtgg cacgagaact acatcgaccc gctggacctg tacttccact tcacgagcat
     2341 cgcgcgcttc accgaggggc tcgacttcgc ccgcgagcgc ttccagcccg tgcgtatcca
     2401 ggcgctcgcg aacgccgacg gcacgccgct gccgcggggc gacctcgtgc tgcgcccggg
     2461 gaaccgcttc acgcgctccg aggactcgcg gttcgaggtc cagcccgaag gcaccgtgac
     2521 caacgcgggc gagctcaacg gcctgctcca cggccagggc cacactgacc tgcggaaccc
     2581 gcccacattc gtggcggagt tccccgagga atcccagttc acgatgcaca tcggccgagt
     2641 aagcaacagc gggcatgtgc gcatctggct cgatggtgcc ctcgtcaacg agacggacct
     2701 gccctgcggc gggaacatcg gcacctcgtg gaagtaccag gagcagtgga agctctggga
     2761 gtcggtctac gacacagaca tcaccattcc agtcccggcg gggcggcacg agatcaaggt
     2821 cgacaacgac gggcgcgact gggtggcggt caccgagtac cgcttcggcg gcctgctccc
     2881 gccgggaagc ggcgccctcg tggccgaggg catgcgcggc gagcacacgg ccgtgctgtg
     2941 gctacagaac ccggccagca cgtggaaggc ccaggccacg ggggccgagg tcgcgcccgt
     3001 tggcccgctc cggctcaccc tcgccgatct gcccgacggc ccatgcactg tggagttctg
     3061 ggagacctgg aagggcgccc tgcaggagac gcgccaggcg acggtcgcgg gcggccggct
     3121 cgctctcgac ctccccgcta tcgcgtccga catcgcgctg aaggtgcgtt actgaaggga
     3181 gcccccctac cccgtcgtcc ctgaggagcc gcgcccgcgc ggcgtcacga agggcgacgg
     3241 gccgcggccc ggataccact caggcctccc tgcggtggca caggcgtccc tgcccggttg
     3301 ggtggggcgg ctgccgctgc cccattggcg gtaactgagg gctcccgtgg cccatcagcc
     3361 tggttgcgga ggggcgc
//
LOCUS       DAOSPL010000348         3376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28228_length_3376_cov_3.126769, whole genome shotgun sequence.
ACCESSION   DAOSPL010000348 DAOSPL010000000
VERSION     DAOSPL010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28228_length_3376_cov_3.126769"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..2686
                     /locus_tag="PLQ54_07610"
     CDS             314..2686
                     /locus_tag="PLQ54_07610"
                     /inference="COORDINATES: protein motif:HMM:NF027541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HOS93165.1"
                     /translation="MLACLVLAASSTGAVGDPAVVFDPAALGYELRCTVEALQGLVNR
                     TGPRLFLGTPDDPWLDIYAERNGIVYETETDLAALFRRFGGEARGLVVYDPEVDGGRY
                     VAATIAGAEDLLPVAPEFLSEASPIGALGLPTVRDLRGQFADSVAAYDWAIAEVFPEC
                     NRHLGLAVDATCEGIVTGLCGPLAGLDWSVQNRGVVFNLGVCPEPMPSYGDTKVGGSP
                     EQAACYRRILAALEAPAQINGYGEPEDYWCKLISEYGHYSFHYGYNWSFHSKVPETRP
                     LKQRVSYTPENVTPETDRYAVCFMTSEGDTMKGPLPFFMGSWFDTERGKVPVNWGINP
                     LMADLFPAMLSYYYDTATEQDCFFAGCSGAGYAYPDYMPNLEQFASVTGEMCRKADIS
                     CVDLWMAAKDDVRRRYAEASGAMGLSANVGRARFTMPIPGVPMIDHGLMYWQTTATAP
                     ETWFKAFADDTKRAKAIDWLVGRIEDIARHHEAPFVILVYGDLHSYAHHATCYREVAD
                     QLDPARFRPMRLDEAMAGLAAWSKGRVLVDAGGINERIQWAALEGVPTGVPLGLVSNA
                     SEPADARITARVGQFQTRTAVNVAPGARTSADGLMLLLAGGPTTREADLTIEGPWGED
                     VRHPTVTLVPANGVAASSASLVGVWDAVSLNHSGGMERPEPDALYGRAWASPDGEGSP
                     QSHIICGPYVDAPAGRCLAAFRLKLLAPMNDPEAVVATLDVNPGGYEGTGKPLGTRPL
                     RAREFAAVGEWQWFAIEVDWPGAPNRMETRVYWAAKAGLAVDRVAAFALE"
     gene            complement(2723..2998)
                     /locus_tag="PLQ54_07615"
     CDS             complement(2723..2998)
                     /locus_tag="PLQ54_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93166.1"
                     /translation="MNRAHADLAISVSLTLHDRVGREETEEQPAARWAAVRFTTPARP
                     EGQRSVLWAVADLGHRYDVRFLIWFVCRDHDALWTRAQDQAPDILVL"
BASE COUNT          536 a         1111 c         1204 g          525 t
ORIGIN      
        1 ccccaggccc gggttggccg gtccaaggtc gatccacagc aactcggccg gagcttgggc
       61 gatcagcggc gttgcggcaa tgagtgcggc gcagagcagc atcacgtagc ctcccgagcg
      121 cgcgccgatg cgcggcatga ccattctacg cgccgcatcc ctcctcccgc caggcgcttg
      181 ggtcggcatg cgtcgcgagt cgggacccac ggaggcccgc tcgacggctg gcatggggcg
      241 cggaggacgc tccgggcgcc cggccgaaca cacgagccac ttggcccgcc gccgatgggc
      301 tccggaggtc atcatgctgg cttgtctcgt tctcgccgcg tcatcgacgg gcgccgtggg
      361 ggatcccgcg gtcgtgttcg acccggccgc tcttggctac gagcttcgct gcacggtgga
      421 ggcactacag ggcctcgtga accgaacggg gcctcgcctg ttcctcggca cgcccgatga
      481 cccctggctg gacatctacg ccgagcgcaa tgggatcgtg tatgagaccg agaccgacct
      541 ggctgcccta ttccgcaggt tcggcggcga ggcccggggg ctggtcgtgt atgaccccga
      601 ggtcgacggc ggtcgctacg tcgccgcgac catcgcgggg gcggaggacc tgctgccggt
      661 ggcgcctgag ttcctctccg aggcctcgcc catcggggcg ctggggctgc cgaccgtgcg
      721 cgatctgcgg ggccagttcg cggacagcgt tgccgcctac gattgggcca tcgccgaggt
      781 gttccccgag tgcaaccggc acctggggct ggccgtggac gccacgtgcg agggcatcgt
      841 cacgggcctg tgcggcccgc tggctggact ggactggtcg gtgcagaacc gcggggttgt
      901 tttcaacctc ggcgtgtgcc cggagcccat gccgagctat ggcgacacca aggtgggcgg
      961 gagccccgag caggccgcgt gctaccggcg gatcctcgcg gcactcgaag ccccggcgca
     1021 gatcaacggc tacggcgaac ccgaggacta ctggtgcaag ctgatcagcg agtacggaca
     1081 ctactcgttc cactacgggt ataactggag tttccacagc aaggtccccg agacacgtcc
     1141 actcaagcag agggtcagct acacgcccga gaacgtgacg ccggagaccg atcgctacgc
     1201 ggtgtgcttc atgaccagcg agggcgacac aatgaagggg ccgctgccct tcttcatggg
     1261 gagctggttc gacaccgaac ggggcaaggt gccggtgaac tggggcatca acccgctgat
     1321 ggccgatctg ttccctgcca tgctcagcta ctactacgac acggcaacag agcaggactg
     1381 cttcttcgcc gggtgttcgg gggccggcta cgcgtacccc gactacatgc cgaacctcga
     1441 gcagttcgcg agcgtcaccg gcgagatgtg ccggaaggcg gacatcagct gcgtcgacct
     1501 gtggatggcg gcgaaggacg acgtgcggcg gcgctacgcg gaggcctcgg gcgccatggg
     1561 tctcagcgcc aacgtcggcc gcgcccggtt caccatgccg atacccggcg tgccgatgat
     1621 cgatcacggg ctcatgtact ggcagaccac ggcgaccgcg ccggagacgt ggttcaaggc
     1681 cttcgcggat gacaccaagc gcgccaaggc catcgactgg cttgtggggc gcatcgagga
     1741 catcgcccgg caccacgagg cgcccttcgt gatcctcgtc tatggtgacc tgcacagcta
     1801 cgcccaccac gccacgtgct accgcgaggt ggcggaccag ctcgacccgg cgcgtttcag
     1861 gcccatgcgg ctcgatgagg ccatggcagg acttgcggcg tggtcgaagg ggcgcgtgct
     1921 ggtcgacgcc ggcggaatca acgagcgcat ccagtgggcg gcgctcgagg gggtgccaac
     1981 cggagttccg ctcggcctgg tcagcaacgc aagcgagccg gcggatgcgc ggatcacggc
     2041 acgcgtgggc cagttccaaa ctcgaacggc tgtcaacgtc gcgcccggcg cccggacatc
     2101 ggccgacggt ctgatgctgt tgctcgcggg cggcccaacg acccgcgagg ctgatctcac
     2161 catcgaaggc ccctggggcg aggatgtgcg ccatccgacg gtgaccctgg ttccggccaa
     2221 cggtgtggcc gcgagcagtg cctcactggt cggcgtgtgg gacgccgtga gcctgaacca
     2281 ttccggcggc atggagcggc ctgagcccga cgcgctctac ggccgcgcgt gggcctcacc
     2341 cgatggagag ggctcaccgc agagtcacat catctgcggc ccctacgtcg atgcgccggc
     2401 agggcggtgc ctggcggcct tccggctgaa gctcctggcg ccaatgaacg atccggaggc
     2461 cgtggtagcg acgctggacg tgaatcccgg cggctacgag ggcacgggga agccattggg
     2521 tactcgcccg ctacgggcca gggagttcgc cgccgtcggc gagtggcagt ggttcgcgat
     2581 cgaagtcgac tggcctggcg cgccgaaccg gatggaaacc cgcgtctact gggccgccaa
     2641 ggccggcctg gccgtcgacc gcgtggcggc gtttgcattg gagtagggca ggggcgggga
     2701 cgtcgggaag cccacagtcg cgtcagagta cgaggatgtc cggtgcctgg tcctgggccc
     2761 tggtccagag cgcgtcgtgg tcgcggcaca cgaaccagat gaggaaccgg acatcgtacc
     2821 ggtgcccgag atccgccacc gcccacagca cggaccgctg accttcgggt ctcgcggggg
     2881 tcgtaaagcg caccgccgcc caacgcgccg cgggctgttc ctcggtctct tcgcgcccca
     2941 cccggtcgtg caacgtgagc gacacagaga tcgccagatc ggcgtgcgca cggttcatgg
     3001 ccgcgtagac ctgccggtgg aggcggacgt aactcggcca ctcgtcggtt gagttccgga
     3061 acggctcgtt ggcctggatg tgttcatgca ggacacggtg cagacgcgcg acgagtcgga
     3121 gctcttcgca tggggggcca tgtccggcaa cacgccgctt ccgtctgcgt gatgcatccc
     3181 gaaggcatgg gggattcgat gcggaatgtc agggctcgcc cgcggtggtc ctcggttcta
     3241 ccgcctaact ggccgccaga cctgagcttc ggggatgccg aggccgtcga ggagaccctc
     3301 gacatactcg gtggtgacca ggtggctgat gtcgtgggcg tcggatgcga ctgagaagag
     3361 agcgcccgcg tcgcgg
//
LOCUS       DAOSPL010000349         3369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28290_length_3369_cov_3.265540, whole genome shotgun sequence.
ACCESSION   DAOSPL010000349 DAOSPL010000000
VERSION     DAOSPL010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28290_length_3369_cov_3.265540"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..1862
                     /locus_tag="PLQ54_07620"
     CDS             21..1862
                     /locus_tag="PLQ54_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS93167.1"
                     /translation="MPGPGPGRGPFAHMVDAAKPENVRAVLFRMWSYLGRYWSRLVIV
                     SLLVAGSTGLGLLGPALTRRAIDGCIAPGRMEGLATVVGLMIACSILSAAFAWMQTII
                     MVRVSQETLLDIRRDLFRRLQALPLRFFDSHPHGEVMSRITNDTEAINSALANTVIQL
                     VGCVLGIVGAGAAMVAMNWRFAIVAVATTPLVFALTRWIAKASREEFRQRQEDLGVLN
                     GLIEETITGQRVVRAFRGERRAIADFEGANSRLRRSATRAAIVAGAMGPFMNLSRNIA
                     FAILACAGGWMVLRQWVTVGTVAAFLSYAEQFSRPINALASLWASVQFAIAGAERVFA
                     IMDEPPEPEDPPDAAALVPVRGDVTFDHVDFGYLPEQPVLRDVTFHAQAGQTIALVGP
                     TGAGKTTIINLLTRFYDVGSGSILIDGCDIRHVRRDELRRALGVVLQDTFVFADTVRG
                     NIRYGRLDATDEEIEEAARLANADGFIRHLPHGLDTPLSEAGGSLSQGQRQLLAIARA
                     VLADPAILILDEATSSVDTRTEIHIQEAMRRLMRGRTAFVIAHRLATVRQADCILVID
                     GGRVVEQGSHAELMAARGAYWQLHELQFGNVVQAAPQGSAEAQRASG"
     gene            complement(1852..2328)
                     /locus_tag="PLQ54_07625"
     CDS             complement(1852..2328)
                     /locus_tag="PLQ54_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93168.1"
                     /translation="MGICQAVLRAAIVLVPTILAGCNPVGWITDQLETGDILDKLAGE
                     TRIQYTVQMSGGAAIAGATMSFYALTTGGDFTNIDDWETVADASVTTGSDGTVIVRVL
                     SSPNEAGDAIVRPETHYRIVYEAPGFRRSVASQAPYDLNTGLKSKTLELTPETLSR"
     gene            2477..>3369
                     /locus_tag="PLQ54_07630"
     CDS             2477..>3369
                     /locus_tag="PLQ54_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOS93169.1"
                     /translation="MRELLTMWQSTKMVVLAALTAALYGGLTVPFKALVILPGFTEIR
                     PTVVIPVLMGIIAGPAGAWGAAIGNLIGDILGGTQGPSSAFGFIGNLGFAYTAYKLYR
                     GPVSRELALAEVPNEMGATGRGPAPTWLGRLILGVLGALVAVAAVVTWSPGEGSQAGW
                     THYAGRVALLLAGAGLVATATLRRWGESMLPVLPTAVASSMMCGGIIGFGTEVLGSQP
                     FGFLASIIAINNAVAIALLLPLLLPAVLPRAKRWGLLVADVLPPDDISVPRAPRMGRC
                     LMWIGLVGLYAVGLGLYSGEFA"
BASE COUNT          517 a         1069 c         1187 g          596 t
ORIGIN      
        1 cggcaggaca tcccggcccc atgcccggcc ccggcccggg gcgtggtcct ttcgcgcaca
       61 tggtcgacgc tgccaaaccc gaaaacgtgc gtgcagtgct gttccgcatg tggtcgtatc
      121 tgggccggta ctggtctcgg ctggtcatcg tgtctctgct ggtcgcggga agcacgggct
      181 tgggactgct tggtccggcc ctgacccgtc gcgccatcga cggatgcatc gcgccgggtc
      241 gcatggaggg cctggccacg gtcgtggggc tgatgattgc gtgcagcatc ctctcagcgg
      301 ctttcgcgtg gatgcaaacc atcatcatgg tgcgtgtctc acaggagaca ctcctcgata
      361 tccgccgcga cctgttccgc cgattgcagg ccctgccgct ccggttcttc gacagccatc
      421 ctcatggcga ggtcatgagc cgtatcacca acgacaccga agccattaac agcgcgttgg
      481 cgaacacggt gatccagctg gtggggtgcg tgctgggaat cgtgggcgcg ggcgccgcga
      541 tggtggcgat gaactggcga ttcgccatcg tggcggtggc gacgacgccg ctggtgttcg
      601 cgctgacacg gtggatcgcc aaggcgagtc gcgaggagtt ccgtcaacgg caggaggact
      661 tgggtgtcct gaacgggctg atcgaggaga ccatcaccgg ccaacgggtg gtgcgggcgt
      721 ttcggggaga gcggcgggcc atcgccgact tcgagggcgc taactcgcgc ctgaggcgga
      781 gcgcgacgcg cgcggccatc gtggccggcg ccatggggcc tttcatgaac ctctcgcgga
      841 acatcgcctt cgccatactc gcatgtgcgg gagggtggat ggtacttcgc cagtgggtga
      901 cggtggggac ggtggcggct ttcctgagct acgcggagca gttctctcgt ccgatcaacg
      961 cgctggcgag cctgtgggcc tcggtccagt tcgccatcgc gggggccgag cgcgtgttcg
     1021 ccatcatgga tgaacctccg gagccggagg acccgcccga cgcggcggcg ctggtgccag
     1081 tgcgcggcga tgtgaccttc gaccacgtgg acttcgggta tctcccagag cagccggtgc
     1141 tgcgcgacgt cacctttcac gcccaggccg gacagaccat cgcgctggtc ggccccacgg
     1201 gcgcgggcaa gacgaccatc atcaacctac tcacgcgctt ctatgatgtg ggctcgggat
     1261 cgattctgat cgacgggtgc gacatccggc atgtgcggcg cgatgaactc cgccgggccc
     1321 tgggggtcgt gctgcaggac acctttgtct tcgccgacac cgtgcggggc aacatccgct
     1381 atggccggct cgacgcgacc gatgaggaga tcgaggaagc ggcgcggctg gccaatgcgg
     1441 acgggttcat ccgccatctg ccgcacgggc tggatacgcc gctctcggag gccggcggca
     1501 gtctgagcca ggggcaacgc cagctgctag ccatcgcgcg tgccgtgctg gcggacccgg
     1561 cgatcctgat cctcgatgag gccacgagca gcgtcgacac gcggaccgag attcacatcc
     1621 aggaagccat gcgacgactc atgcgcgggc gcacggcgtt cgtgatcgcc catcgactgg
     1681 cgacagtacg gcaagccgac tgcattctgg tgattgacgg cggccgcgtc gttgagcaag
     1741 gtagccacgc cgagctgatg gccgcgcgcg gggcctactg gcaactacac gagctgcagt
     1801 tcggcaacgt ggtgcaggcg gccccccagg gttcggcaga ggcccagcga gctagcggct
     1861 gagtgtctca ggcgtgagct cgagcgtctt tgacttgagc cccgtgttga ggtcataggg
     1921 ggcctgggac gcgaccgacc gccggaaacc cggagcttca tagacgatgc gatagtgggt
     1981 ctccggacgc acgatggcgt caccggcctc gttgggcgac gacagcacgc gcacaatgac
     2041 cgtgccatcg ctgcccgtgg tcaccgaggc gtcggccacc gtctcccagt catcgatgtt
     2101 ggtgaagtcg cccccggtgg tcagggcata gaagctcatc gtagcccccg cgatggccgc
     2161 gcccccggac atctgaaccg tgtactgaat ccgggtctcc cccgccagct tgtcgagaat
     2221 gtcacccgtc tcgagctggt ccgtgatcca gcctaccgga ttgcacccgg cgaggatcgt
     2281 gggcaccaaa acgatggcgg cgcgcaacac agcctggcag atccccatga agccctccac
     2341 agtccccgaa tgagactgca agtaggttct ctatggtcgc caggcctcct tccgagggcg
     2401 gcgcaccgcc acggcatcgg caggagggtt cccggcgcaa cacgaaacag attccgccac
     2461 gagatggagg ttcccggtgc gcgagctgtt gacgatgtgg cagagcacga agatggtcgt
     2521 gcttgccgct ctgaccgcgg cgctgtatgg cggactgacg gtgcccttca aggcgctggt
     2581 catccttccg ggattcacgg agatccggcc gacagtggtg atcccggtac tgatggggat
     2641 catcgcgggc ccggcggggg cgtggggggc cgccatcggc aacctgatcg gagacatcct
     2701 cggggggacc cagggcccat cgtctgcctt cggattcatc ggcaacctgg gattcgcgta
     2761 caccgcgtac aagctgtatc gggggccggt gtctcgcgag ctggccctcg cagaggtccc
     2821 gaatgagatg ggcgcgacgg gaagaggtcc ggcccccacc tggcttggcc gactgatcct
     2881 cggcgtgctc ggcgccctcg tggctgtggc ggccgtggtg acgtggtcgc caggcgaggg
     2941 gagtcaggcc gggtggacgc actatgccgg acgagtggcg ctcctgttgg ccggggccgg
     3001 cctggtcgcc acggccaccc tgcggcgctg gggggagagc atgctccccg tgctgcccac
     3061 ggccgttgcg agttcaatga tgtgcggcgg gatcatcggc ttcggcaccg aggtgctggg
     3121 tagccagccg ttcggcttcc tggcgtccat catcgccatc aacaacgcgg tggcgatcgc
     3181 cttgttgctc ccgttgctcc tgcccgcggt tctgccgcgc gcgaagcgat gggggctcct
     3241 ggtggcggat gttcttccgc ccgatgacat tagcgtccct cgggcgccgc gcatggggcg
     3301 gtgtctgatg tggatcggac tcgttggtct gtacgccgtc gggctgggtc tctactcggg
     3361 tgagttcgc
//
LOCUS       DAOSPL010000350         3365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28337_length_3365_cov_3.275227, whole genome shotgun sequence.
ACCESSION   DAOSPL010000350 DAOSPL010000000
VERSION     DAOSPL010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28337_length_3365_cov_3.275227"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..878
                     /locus_tag="PLQ54_07635"
     CDS             114..878
                     /locus_tag="PLQ54_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93170.1"
                     /translation="MRDRVIPVPTAVAAGFVLMGLVTFLAPVRSQTGPVIGLVNMARV
                     QRYARAIQTARDQRAQERLDRQTLLDRHEQFAMLVGDEARRAVELDLKDVRRTAQESQ
                     ELLALRRKSSEMDLDRVKLIGRANPSDQEAARRDELQAVYNRRCDELEALRKQLEEEL
                     LRVDERLQKEVDDAFAAALRSIAEQTGVQVIMASHILVPQPGVEGRDAGYRWENVVYY
                     GGQDVTDALITTMNGGEAPPPPSPDEQGIDLPGTTR"
     gene            1193..1957
                     /locus_tag="PLQ54_07640"
     CDS             1193..1957
                     /locus_tag="PLQ54_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOS93171.1"
                     /translation="MRDRMIPLRTAVVGGFVIVGLVAFLAPVRSQAGPSVGFVNMVRV
                     QKYVKVFQAARDQLFQEQRSRLELLDRHRQFAFLVGDEAKRAVELDMKSVQRTNEENQ
                     ELTALRRKTSDMELEHVKLVGRNNPSEQDMARLNELQTIRNKRADEIDALQQQLQAEL
                     SQAEERLQKDADDRFVAALKSTAEQNGLQIIVASHILVPQNFADGRPTDLRWENVVYY
                     GGRDVTDSLITVMNGGEAPPAPGPDEQGTIIPGAVK"
     gene            2127..3116
                     /locus_tag="PLQ54_07645"
     CDS             2127..3116
                     /locus_tag="PLQ54_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93172.1"
                     /translation="MSRRGYANVESLASEHPMWRALLDARASLAEAVASTPPPRRHPD
                     AVEPSYLLEPEPGRVADTEAWRQEAAASVNTAPASPRPTTGPTAAETDRSAARLAERV
                     ETLSQATGRQADEATQRIAAGRRARSGAARTASAARGAGEWARREARSEAARLRAESA
                     RELDQAEREEREALRGSTSSAEQRWVARAKTRTGEADRVAGFMPDSGAAQGGKAVQVD
                     TRRAHESDRALTARSAQIERERAAVASHRVASLEAQVTTLHARILGDIERLSRAVARQ
                     SHIELVFEPAPGIPDVTDEIRGLLRRMYRLDPHGSEPGDQPARPGGADETNAE"
     gene            3157..>3365
                     /locus_tag="PLQ54_07650"
     CDS             3157..>3365
                     /locus_tag="PLQ54_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HOS93173.1"
                     /translation="MPGDALVLLLRQVLELVDGTTDLPPDLELDGVASLDRARPGHVA
                     FADTGPPERLLACQASAILVRRDFPP"
BASE COUNT          550 a         1128 c         1185 g          502 t
ORIGIN      
        1 ccctgcggac tccgctcgga cccggcgcat gatcggagca gttccctgcc gcgggcgtgg
       61 cgcgcaggcg ccaccccgcg ggcgcggcaa tgtcgtggaa cggaggcaga gccatgcgag
      121 atcgggtgat cccggtgccc accgcggtgg cggccgggtt cgttctcatg ggcttggtca
      181 cattcctggc ccctgtgcgg tcccagaccg ggccagtgat cggcctcgtg aacatggcgc
      241 gcgttcagcg gtatgcccgg gcgattcaga ccgcgcgcga ccagcgcgcc caagagcgcc
      301 tggaccgtca gacgctgctc gaccggcacg agcagttcgc catgctggtc ggtgacgagg
      361 ccaggcgggc cgtcgaactc gacctgaaag acgttcggcg cacggcccag gagagccagg
      421 agctgctggc gctccgccgg aagagctccg agatggatct cgaccgcgtg aagctgatcg
      481 gccgtgccaa cccgagcgac caggaggccg cgcggcggga tgaactccaa gccgtgtaca
      541 accgccgctg tgatgagctc gaagcactcc gcaagcagct cgaggaggag ctgttgcggg
      601 tggacgagcg cctccagaag gaggtcgacg atgcgttcgc ggcggcgctc agatccatcg
      661 ccgaacagac cggcgttcag gtcatcatgg catcgcacat cctggtgccc caaccgggcg
      721 tcgaggggcg cgacgcgggg taccgatggg agaatgttgt gtactacgga ggccaggatg
      781 ttaccgacgc cctgatcacc accatgaacg gtggtgaggc tcctcctcca ccgtctccag
      841 acgagcaggg gatcgatctc ccgggtacga cgcggtagct cggtcattcg ccgcggggat
      901 gcgtcggccc ggggcgggag cctcatgacg tcgccccgcc ggcacgcctt ggcgtccccg
      961 tccgcccttc gataggccca gagcggatgg ggacgggggt ccatgagggg gggtgcggtg
     1021 gattctcggc acccgggttt tctgctatca tgcaccgtgc gtgcgatagc cggcgctgca
     1081 ccggatgccg cctgagccca cgtcgtactt gacgtcgctc ccgatccgtg cctgagtggg
     1141 tgtgcgtgtg taccgctcgg cctggagggg gcggcgaaac ggaggcatag caatgcgaga
     1201 tcggatgatc ccactgcgga ctgccgtggt gggaggcttt gtgatcgtgg gcttggtcgc
     1261 cttcctggca ccggtgcgct cgcaggcggg gccgtctgtt ggcttcgtca acatggtgcg
     1321 ggtgcagaag tacgtgaaag tgttccaggc ggcgcgcgat cagctcttcc aggagcagag
     1381 gtctcgcctc gagcttctcg atcggcatcg ccagttcgcc tttctggtgg gcgatgaggc
     1441 caagcgagcc gtggagctgg acatgaagag cgtccagcgc acgaatgagg agaaccagga
     1501 gctcaccgcg ctccgccgga agacctcgga catggaactc gaacacgtga agctcgtggg
     1561 gcgcaacaat cccagcgagc aggacatggc ccggctcaac gagctccaga ccatccggaa
     1621 caagcgggcc gacgagatcg atgccctcca gcagcagctt caggccgagc tctcgcaggc
     1681 cgaggagagg ctgcagaagg atgccgacga ccggttcgtt gcggctctga agtcaacggc
     1741 cgagcagaac ggcctccaga tcatcgtggc ctcgcatatc ctggtcccac agaatttcgc
     1801 cgacgggcgc cccacggacc tgcgatggga gaatgtggtc tactacggtg gccgcgatgt
     1861 caccgatagc ttgatcacgg tgatgaacgg cggcgaggcg ccacccgcac cggggccgga
     1921 cgaacaggga accatcattc ccggagcggt gaagtagtct cacgaagccc tgcgtcgcac
     1981 cgggtgagcc gcacacggtc gtgataggca tgagccaatc cctgttgcgc cgcacaggcc
     2041 tccttctgct ggtggtcctc ggcgcctcgg ttgtgcttgg gtgctctggt tgccggcgcg
     2101 accgccctct ggtcgagtca gagccggtgt ctcgacgagg ctacgccaac gtggagtctc
     2161 tcgcgtccga gcaccccatg tggcgggcct tgctcgatgc gcgagcctcg ttggcggagg
     2221 ccgttgccag tacgccgccg cctcggaggc accccgacgc tgttgagccc tcctatctgc
     2281 tcgaacccga acccggccgt gttgcggaca cggaagcgtg gcgacaggaa gcggcggctt
     2341 cggtgaacac ggcgcccgca tcgccccggc caacgaccgg ccccacggcc gcggagaccg
     2401 accggtcggc cgcccggctg gccgagcgtg tcgagaccct cagccaggcg acgggccgcc
     2461 aggccgacga ggctacccag cggatcgctg ccggccgccg ggctcgatcc ggggcggcgc
     2521 gcacggcatc ggccgcccgg ggcgccggcg agtgggcccg tcgcgaggca cggtccgaag
     2581 cggcccgcct gcgggcggaa tctgctcgcg agctcgatca ggcggagcgc gaggagcgcg
     2641 aggcgcttcg gggaagcacc tccagcgccg agcagcgctg ggtggcgcga gcaaagaccc
     2701 gcaccggcga ggccgaccgc gttgcaggct tcatgccgga ctctggcgct gcccaggggg
     2761 gcaaggctgt gcaggtcgac acgcgtcgcg cccatgagtc cgatcgggca ctgacggcga
     2821 ggtcggcgca gatcgagcgc gagcgcgcgg ctgtggccag ccaccgggtg gcctcgctcg
     2881 aggcccaagt caccacactg cacgcacgca ttctgggcga catcgagcgg ctgtcccggg
     2941 ccgtcgcgcg ccagagccat atcgagctcg tgttcgagcc agccccaggg atacccgacg
     3001 tcaccgatga gatccgtggg cttctgcgcc gcatgtaccg cctcgacccc cacggctcgg
     3061 agcccgggga ccaaccggca cgcccgggag gcgccgacga aaccaacgcc gaataggccg
     3121 ccggtccttc tgttcacgaa ccaccctaag ctggggatgc cgggtgatgc tctggtgctt
     3181 ctgctccgcc aggtgctcga actcgtcgac ggcacgactg acctgccgcc cgacctggaa
     3241 ctggacggcg tggcgagtct cgatcgagcg cgccccggcc acgtggcatt cgccgacacg
     3301 ggccctcccg aacgcctact agcgtgccag gcttccgcca tcctggtgcg acgcgacttc
     3361 ccgcc
//
LOCUS       DAOSPL010000351         3365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28338_length_3365_cov_2.803625, whole genome shotgun sequence.
ACCESSION   DAOSPL010000351 DAOSPL010000000
VERSION     DAOSPL010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28338_length_3365_cov_2.803625"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PLQ54_07655"
     CDS             complement(<1..480)
                     /locus_tag="PLQ54_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93174.1"
                     /translation="MPHVTYSLAGGLSDEQIGRMHDEALGLIETVGLHVGHREIRGIL
                     ADHEGVTVDGEVVRFAPDRVEAAVAAQYYPPELSQFDYEMVAGAYALNVTDPGDGHTR
                     PATTDDLRFLVKLADRLGCWGSAPARPTDIPSPELQEIALQRIAWENSPRKDCGPLDA
                     "
     gene            complement(569..1597)
                     /locus_tag="PLQ54_07660"
     CDS             complement(569..1597)
                     /locus_tag="PLQ54_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010043753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOS93175.1"
                     /translation="MTRDRLLELLRGFEKATIGVIGDFFLDKYLIIDPALAERSLETG
                     LEAFQVVEKRPSPGAAGTVCSNLAALRVGRLLAIGAVGDDGDGYELRQGLSARGVDHT
                     HLLSLSERYTPTYTKPMVIDADHPGGEREINRMDVKWRQPTTPEIERAIMAQLDACLD
                     QLDAVVIADQVQERNHGVITDAVREGLAQRAAARPGTVFFVDSRMHIGEFRHAIIKPN
                     SHEAAAAVGLESTDALEDLARGARSLMERSGRPVFVTAGERGIVVVDEGGMAHVPAIP
                     VTGPIDIVGAGDSTTAGIVSALCAGASLTEAALVGNLVASITIQQLGTTGTATPEQVL
                     ERLGAVGP"
     gene            complement(1605..2753)
                     /locus_tag="PLQ54_07665"
     CDS             complement(1605..2753)
                     /locus_tag="PLQ54_07665"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS93176.1"
                     /translation="MTARERVRAAFDCRIPDRVPIYQAGFSATVASQILGRPAYVGGG
                     HAQYLEACALWEGPDAHEEYLTRCWRDAVDLCRALELDVVRTTYWRMPERPTRRIDEH
                     TFAYGDETRGPWRIMRHDPASELYQVVARSPEPEATIDGLEPVVEGAEAHAEAYRPGP
                     ETFDAVLRAQAEFGDDGVPYGGIPVGLGIPREPVWLEATALGPDLVGRYLATQAIIAE
                     RNVALGAALGLTYAYGGGDFCSPRGPMYSPRAFHELVLPQLERVSRACDAAGALHGFA
                     SDGNLWPVADDLFGQSGVRFFYEVDCLAGMDLTRLRTTFPHLALLGGINSATLHQGTV
                     EQVIGETRRALRAAREHGGCVIGCSYQIVAGTPMANFWAMMETLEAER"
BASE COUNT          508 a         1205 c         1154 g          498 t
ORIGIN      
        1 ggcgtccagg gggccgcaat ccttgcgggg gctgttctcc caggcaatgc gctgcagcgc
       61 gatctcctgc aactcggggc tcggtatgtc cgtcggccgc gccggcgccg agccccaaca
      121 acccaagcga tcggccagct tcaccaggaa gcgcagatcg tccgtcgtcg cgggccgggt
      181 gtgaccgtcg ccggggtcgg tgacattgag ggcgtaggcg ccagcgacca tctcgtagtc
      241 gaactggctc agctcgggcg ggtagtactg ggccgccacg gccgcctcga cacggtcagg
      301 agcgaagcgc acgacctcgc catcgaccgt gacgccctcg tggtcggcca ggatgccgcg
      361 gatctcccgg tggcccacat ggaggccaac ggtctcgatg aggcccaggg cttcgtcgtg
      421 catccggccg atctgctcgt cgctcaggcc tccggcaagc gagtacgtga cgtgtggcaa
      481 cgcgtgggtc ctctccttga ccggccccgg cctggcgccc catggcgctc ttggcgccaa
      541 gccaccactg atggtctgac agccgcccct acgggccgac cgcgcccagc cgctcgagta
      601 cctgctcggg cgtggcggtg ccggtggtgc cgagttgctg gatcgtgatc gatgccacca
      661 ggttgccgac gagggccgcc tcggtgagcg aagcccccgc gcagagcgca ctcacgatcc
      721 ccgcggtggt gctgtcgccc gcgccgacga tgtcgatggg cccggtcacc gggatcgccg
      781 ggacgtgagc catcccgccc tcatccacca ccacgattcc ccgttcaccc gcggtgacga
      841 agaccggtcg gcccgagcgc tccatcaggc tccgggctcc gcgggccaga tcctccaggg
      901 cgtcggtcga ctcgagcccc accgccgcgg cggcctcatg agagttcggc ttgatgatgg
      961 cgtgccggaa ctccccgata tgcatccgcg agtcgacgaa gaacaccgtg ccgggccggg
     1021 ccgcggcgcg ctgggccagc ccctcgcgta ccgcatcggt gatgacgccg tggttccgct
     1081 cctgcacctg gtcggcgatg accactgcgt cgagctgatc gaggcacgca tcgagctggg
     1141 ccatgatggc ccgctcgatc tccggtgtcg tgggctgccg ccacttcacg tccatccgat
     1201 tgatctcgcg ctcgccgcca ggatggtcgg cgtcgataac catgggcttg gtgtaggtcg
     1261 gcgtgtagcg ctcgctcagc gacagcaggt gggtgtgatc cacgccgcgc gcggagaggc
     1321 cctgccggag ctcatagccg tccccatcgt cgcccacggc gccaatggcc agcaggcgac
     1381 ccacgcggag tgcggccagg ttgctgcaca cggttcccgc ggcgccgggg ctggggcgct
     1441 tctcgacgac ctggaaggcc tcgagcccgg tctccagcga ccgctcggcc agtgccggat
     1501 cgatgatcaa gtacttgtcg aggaaaaagt ctccaatgac gccgatggtg gccttctcga
     1561 agcctcgcag gagctcaagt agccgatccc gggtcatgtg cgttctacct ctccgcctcc
     1621 agtgtctcca tcatggccca gaagttggcc atgggcgtcc ctgccacgat ctggtacgag
     1681 caaccaatga cgcaccctcc gtgctcacgc gcggcccgga gggcacgccg agtctcgccg
     1741 atcacctgct caacggttcc ctggtgcagc gtcgccgagt tgatgccacc gagcagggcc
     1801 aggtgcggga acgtggtccg cagccgcgtc aggtccatcc cggccaggca gtctacctca
     1861 tagaagaacc gcacaccgga ctggccgaag aggtcatcgg cgacgggcca caggttgcca
     1921 tcgctggcga aaccgtgcaa cgcacccgcg gcgtcgcagg cccgagagac gcgctcgagc
     1981 tgcggcagca ccagctcgtg gaacgcacgg ggcgagtaca tcggcccacg gggcgagcag
     2041 aagtcgcctc cgccgtaggc gtaggtcagc ccgagcgcgg caccgagagc cacgttccgc
     2101 tcggcgatga tggcctgcgt cgccaggtag cgcccgacca ggtcaggccc gagggccgtg
     2161 gcctccagcc acacgggctc tcttggaatg cccaggccga ctgggatgcc gccgtagggc
     2221 acgccgtcgt cgccgaactc ggcctgcgcc cgcaggaccg cgtcgaaggt ctccgggcca
     2281 ggccggtagg cctcggcatg tgcctccgca ccctcgacga cgggctcgag gccatcgatt
     2341 gtggcctcgg gctccgggct gcgggcgact acctggtaga gctcgctggc cggatcgtgc
     2401 cgcatgatcc gccacggtcc gcgtgtctcg tcaccgtagg cgaaggtgtg ctcgtcgatg
     2461 cggcgagtcg gccgctccgg catgcgccag taggtcgtgc gcacgacgtc gagttcgagg
     2521 gcgcggcaga ggtcgacggc gtcgcgccag cagcgggtga ggtactcctc gtgggcatcg
     2581 gggccctccc acagcgcgca ggcctcgaga tactgcgcat ggccaccgcc gacataggcg
     2641 gggcgcccca ggatctgcga ggcgacggta gccgagaacc cggcctggta gatgggcacc
     2701 cggtcgggga tccggcagtc gaaggcggcg cggacgcgct cgcgtgcggt caccccactc
     2761 cacccccaaa gctcccagtg ccggcgggcg tcctcgcccg ccgcccgtgg gtcagaacca
     2821 aacacgcggg ggcccgcggg cccagggact ccgggcatgg ccttgccgca gccccattcg
     2881 cgccccgtgc cacgcgagtc ctcctcaccg cagcggcatc gagggcggcc gcacgcccaa
     2941 cgcgattggg acggccgggc cgccagatgg cctcgggtgc cgtagagccg ggacaggatc
     3001 cccctcgccc gcccggcggc caggcggggg aaccacatgg ccagcgtggc ccatgaaggc
     3061 accatcgcac ggccgagtgg cagcatcggg aagcgcgcga aggcacgtcg ggcaagatca
     3121 ggcatgccgt cagcgtcgcg tcatattccg tgggtcttcc tccagcttgg ccgcgcacgc
     3181 atgtgccaga cagatcccgt ccaaggtttc gatcgctggg gcagaggccc tcgggcggta
     3241 atccccgagt ggaggccgtt gggccccgac ccctctgccc ggggcaggcg ctcctggcgg
     3301 cccactcgaa ccatgtccgt atggctcagc tctctctcgt cgatgcccat gcgcacctcc
     3361 aggag
//
LOCUS       DAOSPL010000352         3361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28375_length_3361_cov_3.670599, whole genome shotgun sequence.
ACCESSION   DAOSPL010000352 DAOSPL010000000
VERSION     DAOSPL010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28375_length_3361_cov_3.670599"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1507)
                     /locus_tag="PLQ54_07670"
     CDS             complement(107..1507)
                     /locus_tag="PLQ54_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93177.1"
                     /translation="MAREMTSSPRETRTDDPILTGLVLLLVVLMPTQRSIKANVTPAD
                     LVLGLAFVWLVGSAVWRFLLRRPNRPSLLWPPVPFWIYLFVAGLSCLRLPSDLEHILQ
                     DAGSQYQAPLRILVAKEILQTVELFFIAPLVLVNGLRTRRMTAVAACLLVGVTCLVVL
                     WGGAQAGSHGSAEAVRATFGSRLAYGGFLAVALPLVWALALGLSAPGGAMRMTLISVG
                     LCAMVFVGLYSMPAFGPFWAAALGLVVVSSALPGSAGARWGRTAAALVLIVVPWALAP
                     HRSEFRTPSVALQVTNEDGEKAIADRYAEWQAAHAMTMANEVGGTVTQEPGYPLYAGG
                     NAVVGLGPGRYQDKISTYGEFLPVNKIEADSQNQYLVIAPTLGLTGVFAFAILLGEGV
                     RSGVRGREDETNPPLARVVSAGLVGSLASLAVACLYLTALVRGLGLVVMLVLVMAMTL
                     GPRPAEPPTPPGPDGE"
     gene            complement(1497..2180)
                     /locus_tag="PLQ54_07675"
     CDS             complement(1497..2180)
                     /locus_tag="PLQ54_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93178.1"
                     /translation="MTNAQAVPESSPGESPGLGTRAAETLAEGLVLILAAMAVVDRVP
                     LPDGYALPMLILAGLLVVLLVARRSAGLLRPDVGMVTALGLAVLLTCAKGSRRIGEVE
                     LFPIEGFGGVPVPWAEPVGLAFVFVVVARSLLRRGQDLRQSPYAPSVLVAAVCLVAVE
                     LVLATSVRRAEATLPMDVPLAFALHTLEFAALLWLCSTLTRLGMARRLALIAVLGLVA
                     KVVWASHGA"
     gene            complement(2275..>3361)
                     /locus_tag="PLQ54_07680"
     CDS             complement(2275..>3361)
                     /locus_tag="PLQ54_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93179.1"
                     /translation="NYGGDKLWPAPQGPADGRHWPGPGDPVLDGGRFESQILRPGGDT
                     AAVRLVSPPDTAYTGVRFSREITLHAGESRVHIVSGMRNVIDRPVRWSIWEVAQIDAA
                     GPAGPGYNDALSAYVPASPVSRHPRGFYVMYGLWRNPAWQRPTPRSPIRVDFSYQIGK
                     IGVDSDAGWLSVIDGRAGRTFTQVFPHEPGTAYPDQGSSVEFWINGAGSFNFLGSHVS
                     VPADPRMTPPYLEAEVLSPLVTLAPGEEAEAAMTWGLGSAQGEPISATSEALVCGPAE
                     ANADAGAVRVRMALTPFIGGRLHAVLVDGQGQVVAERELAVAVPGARRCVDVRMADRS
                     GAAAGARVVIVDRAGKSWTLAAMPVNR"
BASE COUNT          606 a         1173 c         1132 g          450 t
ORIGIN      
        1 tccccttcag cttgcgctgg cctgccaccc ggccatgcgc acgtacctct agacactgaa
       61 tgcgccagaa ggttccgttg aacgggtgtc caggtggctc agctggtcat tcgccgtcgg
      121 gcccgggagg agtcggtggc tcggccgggc gaggtcccag cgtcatggcc atcaccagga
      181 cgagcatgac gaccagtccg agtccgcgta ccagggccgt caagtacaga caggccacgg
      241 cgaggctggc cagtgagccc accaggcccg ccgagaccac tcgggcaagg ggcgggttcg
      301 tttcgtcttc gcggccgcgc acgcccgagc gaacaccctc tcccagaaga atcgcgaaag
      361 caaacacccc ggtcaggccg agcgttgggg cgatgaccag gtactggttc tgtgagtccg
      421 cctcgatctt gttgacgggg aggaactcgc cataggtcga tatcttgtcc tgatagcggc
      481 cgggcccgag tcccaccacc gcgttgccgc cggcgtagag ggggtagccg ggttcctggg
      541 tcaccgtccc gccgacctcg ttcgccatgg tcatcgcgtg ggcggcctgc cactcggcat
      601 accgatcggc aatggctttc tcgccgtcct cgttagtcac ctggagcgcc acactgggcg
      661 tgcggaactc cgaccggtgg ggcgcgaggg cccatgggac cacgatgagc accagggcag
      721 ccgccgtgcg gccccaacgc gctccggcgc tgcccggcag ggctgaggag accaccacca
      781 ggcccaaagc ggcggcccag aacggcccga aggccggcat cgagtacagg cccacgaata
      841 ccatggcgca caggcccaca ctgatcagcg tcatgcgcat ggcgccgccg ggggccgaga
      901 ggccaagcgc aagcgcccag accaagggga gtgccacggc caggaacccg ccataggcca
      961 agcggctgcc gaaagtggcc ctcaccgcct cggcgctgcc atggctgccg gcctgggcgc
     1021 cgccccacag caccacgagg catgtgacgc ccaccagcag gcaggctgcc actgcagtca
     1081 tgcgtcgagt gcgcaggccg ttcaccagga ccaggggcgc gatgaagaac agctcgaccg
     1141 tctgcaggat ctccttggcc accagtatcc tgaggggggc ctggtactgg cttccggcgt
     1201 cctgaaggat gtgctccagg tccgacggga gccgcaggca tgacaacccg gccacgaaga
     1261 ggtagatcca gaagggcacc ggcggccata gaagactcgg gcgattgggg cgtcggagca
     1321 ggaagcgcca cacggcggac cctacgagcc agacgaaggc gaggcccagg accaggtccg
     1381 ccggcgtcac gttggccttg atggaccgct gagtcggcat gagaacgacc agcaacagca
     1441 ccagacccgt gaggatgggg tcgtccgtgc gggtctctcg aggtgaagat gtcatctcac
     1501 gcgccatggc tggcccacac caccttagcc accaggccaa gcaccgcaat gagcgccagc
     1561 cggcgggcca ttccgaggcg cgtcagtgtg gagcagagcc atagcagcgc ggcgaactcg
     1621 agggtgtgca gggcgaacgc cagtggcaca tccatgggca gtgtggcctc cgcgcgccgg
     1681 acggaggttg ccaacacgag ctcgacggcc accagacaga cggccgcgac caagaccgag
     1741 ggcgcgtagg ggctctggcg caggtcctga ccgcgccgga gcaggctgcg cgcgaccacg
     1801 acgaagacga acgccagccc caccggctca gcccacggga cgggaacgcc gccgaagcct
     1861 tcgatcggga acagctcgac ctcaccgatc cgccgggagc ccttggcaca ggtcagcagg
     1921 accgccaggc ccagagcggt caccatcccc acgtcgggcc tcagcagccc ggccgagcgt
     1981 ctggcaacca gaagcacgac cagaagaccc gcgaggatca gcatgggcag ggcgtagcca
     2041 tcgggaagtg gcacacggtc gaccacggcc atggccgcga ggatgagcac aagcccctcg
     2101 gccagcgtct cggccgcgcg tgtgccgaga cccggggatt cgccgggcga ggactccgga
     2161 actgcttgtg cgttcgtcat cggcaccgcc tcgggtcacc ggtcgttacc ggcacttcag
     2221 tcaggttcac ggtgggcaca cctcagatga taggcaactt ggcgccgccg gccgtcaccg
     2281 gttcacgggc attgccgcta gcgtccacga cttccccgca cggtccacga tgaccacgcg
     2341 ggcgccagca gcggcgcccg atcggtccgc cattcgcacg tcgacgcacc gccgggcgcc
     2401 gggaaccgca acggccagct cgcgttccgc cacgacctga ccttggccgt cgaccaacac
     2461 agcatgcagc cgacccccga tgaatggtgt gagcgccatg cgtacccgga ccgcccctgc
     2521 atcggcattc gcctcggccg gcccgcacac caaagcttcg gaagtggccg agattggctc
     2581 gccttgagca gaccccagac cccaggtcat cgcggcctcg gcctcttccc ccggagccag
     2641 tgtcaccagt gggctgagca cctcggcttc gaggtaggga ggcgtcatcc gcgggtccgc
     2701 gggtacggac acatgactgc ccaaaaagtt gaaactcccg gcgccattga tccagaactc
     2761 caccgaggag ccctggtccg ggtatgcggt accaggttcg tgcgggaaga cttgggtgaa
     2821 tgtccgtccc gctcgcccgt cgatgacgct cagccagccc gcgtccgaat ccacgccgat
     2881 cttgccgatc tggtagctga aatcgacccg gatcggactc cgcggagtgg gtcgctgcca
     2941 ggcggggttc cgccacaggc cgtacatcac atagaagcct cgcgggtggc ggctgaccgg
     3001 cgaggcaggg acgtaggcgc tcagcgcgtc gttgtagccg ggcccggccg ggcccgcggc
     3061 atcgatctgc gcgacttccc agatggacca ccgcacgggc cggtcgatga cgttccgcat
     3121 gccggataca atgtgcaccc gggactcccc cgcgtgaaga gtgatctcgc gagagaaccg
     3181 gacacccgtg taggcggtat cgggtgggct gacgagtcgt accgcggccg tgtccccccc
     3241 ggggcgcagg atctgggact cgaaccgccc gccgtcgagc accggatcgc ccgggccggg
     3301 ccagtgccgg ccgtccgccg gcccctgcgg ggccggccag agcttgtcgc cgccgtagtt
     3361 g
//
LOCUS       DAOSPL010000353         3354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28460_length_3354_cov_3.271901, whole genome shotgun sequence.
ACCESSION   DAOSPL010000353 DAOSPL010000000
VERSION     DAOSPL010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28460_length_3354_cov_3.271901"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLQ54_07685"
     CDS             <1..231
                     /locus_tag="PLQ54_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93180.1"
                     /translation="HPLEADAQVSAEPGTVVAVTREGLRVRCGEGDLLLTRLQPAGGR
                     VMDARAFANGYRVAVGDRFEGEPSTGTGGDLT"
     gene            273..1475
                     /locus_tag="PLQ54_07690"
     CDS             273..1475
                     /locus_tag="PLQ54_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93181.1"
                     /translation="MAVVVGVGRAEPLPVAFAESVGQPAPASTPALAESIKGRRPVLL
                     DFAGDAGPDRVKQDLVLNALLPRYERCMAVVRVWNETEDGDPVFDDYRVKRIPSLCVI
                     GSDGRIATVFEGPVTAEALATALDRVLAAGGVAPPPATAPGASPARPGTPVEQLTGRA
                     LPLVTPAVAQAVAAGRPVALLFHADWGRYSREQVQVLERLRQRWGASVAIELVRDGTP
                     EAVAAFSEFGVRAVPVLVIADSGGAVRHVFPRVASEGEVAACLEALLAAPAPPPAAQL
                     LGEGETPEGSAELVGKRAVASTEILSYALQSERPTVVFFQRARDVQASEVAELLAHVK
                     KAFAEDANVLTVIEGAPGSAAAWADYAVGETPAVVAIGADGVIKRVCLVRPPAEVLIA
                     AIRGAIRP"
     gene            complement(1476..1775)
                     /locus_tag="PLQ54_07695"
     CDS             complement(1476..1775)
                     /locus_tag="PLQ54_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOS93182.1"
                     /translation="MDDPIRRIRLSIDGAVQGVGFRFFISRHAQDLGLCGYVRNMPDG
                     SVEIVAEGAMSRLRQLAERAERGPSMSRVTDVDLEWGPATGEFSGFDIRRGPLGR"
     gene            1909..2580
                     /locus_tag="PLQ54_07700"
     CDS             1909..2580
                     /locus_tag="PLQ54_07700"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOS93183.1"
                     /translation="MVRCLLATGVLAMLTSCAKREEPAPPSAGPGPAEAGTPVPRAAH
                     TGGSQPTVAMIIAHSRFRDEELLAPKQALEKAGARVDVVSTALGEAKGMKGATVQAGR
                     TIDEISPGDYDAIVFVGGTGAQDYWDSKVAHDLCRGTVESGKVLAAICIAPVTLARAG
                     VLEGKQAVVWADCRDELSKRGARLAPPSTHVAVDGRIITGDGPDAAEEFGQELCRALG
                     LGEGA"
     gene            complement(2596..2886)
                     /gene="rpsT"
                     /locus_tag="PLQ54_07705"
     CDS             complement(2596..2886)
                     /gene="rpsT"
                     /locus_tag="PLQ54_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017822404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOS93184.1"
                     /translation="MPLKGTALKRWKIETRNRRRNQAVKTELKTRIKKARTAVASGDE
                     TAAEALKLALRKLDKAATKGVIHKRNAARRKSVLQRLYNKAQQQPASETGAE"
     gene            3130..>3354
                     /locus_tag="PLQ54_07710"
     CDS             3130..>3354
                     /locus_tag="PLQ54_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor MviN"
                     /protein_id="HOS93185.1"
                     /translation="MSPPPDPEAPESSTSSARRQVVIASLILIGAIFVSRISGMARVV
                     AFSYRFGASGMTSAYNQAFGLPDLVNMLIGG"
BASE COUNT          446 a         1121 c         1217 g          570 t
ORIGIN      
        1 catcccctcg aggccgacgc gcaggtctcg gccgaaccgg gcacggtggt cgccgtaacg
       61 cgcgagggtc tgcgggtccg ttgtggcgag ggcgacctgc ttctgacccg gctccagccg
      121 gccggaggac gggtcatgga tgcgcgagcc ttcgccaacg gctatcgcgt ggccgtgggc
      181 gaccggttcg agggcgagcc gagtaccggc accgggggtg acctcacatg atggcatggg
      241 tcatgcgctc tgggccctgg gcggcggtcg ccgtggcagt agtggtggga gtcggccggg
      301 cggagccgct tcccgtcgcc ttcgccgaat cggtaggcca gcccgccccg gccagcactc
      361 ccgcattggc cgaatccatc aagggccgtc ggcccgtgtt gctggacttc gccggtgacg
      421 ccggaccgga tcgggtgaag caggatctgg ttctgaacgc cctgctccct cgctatgagc
      481 gctgcatggc cgtggtccgc gtctggaacg agacggaaga cggggatccc gttttcgacg
      541 attaccgcgt gaagcgcatt ccgagcctgt gtgtgatcgg gtccgatggg cgcatcgcga
      601 cggtgttcga ggggccggtc acggccgagg cgcttgccac agccctcgat cgggttctgg
      661 ccgccggagg ggtggcgccg ccgcccgcga cggcgccggg ggcgagtccg gcgcggccag
      721 gcacgcccgt ggagcagctc acggggcggg ccctgcctct ggtgactccg gccgtggcgc
      781 aagccgtcgc ggcgggccgg cccgtggccc tgttgttcca cgccgactgg ggccgctaca
      841 gtcgggagca ggttcaggtt ctcgagcgcc tgcggcagcg gtggggcgcg tcggtggcta
      901 ttgagctcgt gcgcgacggc acgccggagg ccgtcgcggc cttttccgag tttggcgtgc
      961 gcgccgttcc cgttctggtc atcgccgact cggggggcgc ggtgcgccat gtgttcccac
     1021 gtgtcgcgtc tgagggcgag gtggccgcgt gcctggaggc gctgctcgcg gccccggcgc
     1081 cgccgcccgc ggcccagctc ctcggcgagg gcgagacccc cgagggtagc gccgaactgg
     1141 tgggcaagcg tgcggtcgcg agcaccgaga tcctctcgta cgcgctccag tccgaacggc
     1201 ccacggtcgt gtttttccag agggctcgcg acgtccaggc atccgaagtt gccgagctgc
     1261 tggcccatgt taagaaggcg ttcgcggaag acgccaacgt gctcacggtt atcgagggcg
     1321 cgccgggtag tgccgcggcc tgggcggact atgcagtggg tgagacaccg gcggtggtgg
     1381 cgatcggcgc cgacggtgtc atcaagcggg tgtgcctggt ccggccgccg gccgaagtgc
     1441 tcatcgcggc catccggggg gccattcggc cgtgactagc gccccagggg gccgcgacgg
     1501 atgtcgaacc cgctgaactc tccggtggct ggtccccact cgaggtcgac gtcggtgacc
     1561 ctcgacatgg agggaccgcg ctccgcgcgc tcggccagct ggcgcagacg gctcatggcg
     1621 ccctcggcca cgatctcgac actgccgtcg ggcatgttcc gcacgtagcc acacagcccg
     1681 agatcctggg catggcggct gatgaagaag cggaatccca cgccctgaac ggcgccgtcg
     1741 atactgagcc ggatgcggcg gatcgggtcg tccatcactt accggtcccc cggggtcagc
     1801 actccgcccg acgtgggcca gatggtggcg agcgtcttca caccatgccg ccttggtgat
     1861 aacgtcgtgg ggtagcgcat ggttctcgct gtcccggagg tgtgacccat ggttcgctgc
     1921 ttgctcgcca cgggtgtgct cgcgatgctc accagctgtg ccaagcggga ggaacccgcg
     1981 ccgccgtccg ctggccccgg cccggccgag gccggcactc ccgtcccgag ggcggcccac
     2041 accgggggat cgcaacccac ggtcgccatg atcatcgccc atagccggtt tcgcgacgag
     2101 gaactcctcg cgcccaaaca ggcccttgag aaggcgggtg cccgcgttga cgtggtctcg
     2161 acggccctcg gcgaggccaa ggggatgaag ggtgccacgg tccaggcagg ccgcaccatc
     2221 gacgagatct cgcccggcga ctacgacgcc atcgtcttcg tcggtgggac cggagcccag
     2281 gactactggg actcgaaggt ggcccatgac ctgtgccggg gcaccgtcga gtccggtaag
     2341 gtcctggcgg ccatctgcat cgcgcccgta actctggccc gcgcgggcgt gctcgagggc
     2401 aagcaggccg tggtatgggc cgactgtcgc gacgagctct cgaagcgcgg cgcgaggctc
     2461 gctcccccga gcacccatgt ggcggtcgac ggccgcatca tcactggcga tggcccagat
     2521 gcggccgagg agttcggtca ggagctgtgc cgggccctgg gtctgggcga gggagcctag
     2581 gccgcgaacg ggcgcctact cggcgcctgt ctcgctcgca ggctgctgct gtgccttgtt
     2641 gtacaggcgc tggagcaccg acttgcggcg ggcggcgttt cgcttgtgga tgacgccctt
     2701 ggtggcggcc ttgtcgagct tgcgcagggc gagtttcagc gcctcggccg cggtctcgtc
     2761 gccgctggcg acggccgtcc gtgccttctt gatgcgcgtc ttgagctcgg tcttcaccgc
     2821 ctggttgcgg cggcgattgc gggtctcgat cttccagcgc ttgagtgcgg ttcccttgag
     2881 tggcattatc ggtccttctc tcgagcaacg gccgcttcgg ggctccggcc tggcaggcga
     2941 tcacggagcc acgcgagtga cgagcgacga ataggcgccc cctgccctgc cggtggagca
     3001 gcttgcctcg agcctgaccg aaggtgctcc ggggacaagt gggcattata gcgagcacgt
     3061 tgacactccg caaggtgcct tgctacaatc gcgcggcctg ccggccaggt gtgggagcgt
     3121 tccctgagca tgtcgccacc gccagacccc gaagcccccg agtcgtctac ctcatcggcg
     3181 cggcgacagg tggttatcgc cagtctcatt ctcatcggcg ccatcttcgt cagccgcatc
     3241 tcgggcatgg cgcgtgtggt ggcgttcagt taccgctttg gcgcatcggg catgaccagt
     3301 gcctacaacc aggccttcgg cctgcctgac ctggtcaaca tgctcatcgg cggt
//
LOCUS       DAOSPL010000354         3353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28471_length_3353_cov_2.481504, whole genome shotgun sequence.
ACCESSION   DAOSPL010000354 DAOSPL010000000
VERSION     DAOSPL010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28471_length_3353_cov_2.481504"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2057)
                     /locus_tag="PLQ54_07715"
     CDS             complement(<1..2057)
                     /locus_tag="PLQ54_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93186.1"
                     /translation="MNPFVLLCLAAVGSAAGAQPLQFLRNGTLEVTEGWSLSVGSAVS
                     DAGRRGRCLRVERGVCSQDVVLAAPGGDYTCSVWMKVEQVTPSAPGGFAFAAVYQYSL
                     DGALVEFRDFAQLTEPGGWTRQSFSFRANPLADIVSMRLGFFNASGVAYFDDAVLTPG
                     MDAADPVEVAREQPGLPPRVAILTDPAFPTYGAPSDPAYLAELLQAAGIEVEALTADQ
                     LSSPGQLSPDRQALLVVPTGQTFPAEARRNLVAFLHGGGDLLTTGGYPFQRPLVRDGA
                     GWIAASEWRDRRLQEGLAGQGGLVPDPGFEQDARSTWEAADGNATRVAQGARSGQRCA
                     RVQMEPGQPAGHSMWRCSFTPKTGQDYLVVGWLRTASIEGDGFAYLAVYQYGEDGKLV
                     DFHDICHVRGTTDWARHTYGFRAAGGATRVELQCGIYRASGTAWFDDVVAIESPPRHQ
                     INTSDGQPGDGLVTSPAQIGLCDPSAPLKRVARLTASPGQYVVPPAVSLRDAFEGYMA
                     TGVTGDDQARWVPLLYAYDRLGRPRGAAGALLIHYAGLYAGSTWACFSVESRDVFARG
                     DRTMGETFVRTVRHMLRGLFLTHLKPEPASVSDGEAVVIYAGVRNGGTATQTARLRFV
                     FVGEDGLTPFATEERTVELGPGADERVQARVSPERYPGAFCTVRCELSTAEEPVDLME
                     AGWV"
     gene            2189..2647
                     /locus_tag="PLQ54_07720"
     CDS             2189..2647
                     /locus_tag="PLQ54_07720"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOS93187.1"
                     /translation="MTRDELLAIYSRIPTESTCRDCHECAIRCSGAIPLARSEWEGIA
                     AYIETHMSDLEFESLLAQDKRFEVADGVWEEFCILYDVVALRCAVYPVRPLVCRLLGH
                     VEFMPCPLSRVPSVLPDGPEIMRRYAQLERKTVAEWLAGEPRRGCGPRGA"
     gene            2665..2820
                     /locus_tag="PLQ54_07725"
                     /pseudo
     CDS             2665..2820
                     /locus_tag="PLQ54_07725"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
     gene            2824..3303
                     /locus_tag="PLQ54_07730"
                     /pseudo
     CDS             2824..3303
                     /locus_tag="PLQ54_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
BASE COUNT          590 a         1110 c         1102 g          551 t
ORIGIN      
        1 acccatcccg cctccatgag gtccacgggc tcctccgccg tgctcagctc acagcgaacg
       61 gtgcagaaag ccccggggta gcgctcgggt gagacacggg cctggacacg ctcatcagcc
      121 cccggcccca gctccaccgt ccgctcctcg gtggcgaatg gcgtcagacc gtcctcgccc
      181 acgaagacga accggaggcg ggccgtctgt gtcgcggttc cgccgttgcg cacgcccgca
      241 taaatgacaa ccgcctcacc gtcgctcaca ctggccggct cgggcttcag atgcgtgagg
      301 aacagaccgc ggagcatgtg ccgtaccgtc cgcacgaacg tctcgcccat cgtgcggtcg
      361 ccgcgggcga acacgtcgcg gctctcgacg gagaagcatg cccaggtgga cccggcatag
      421 agccctgcat agtgaatgag caacgcgccc gccgcccctc gaggccggcc gaggcggtcg
      481 tacgcgtaga gcaggggtac ccaccgtgcc tgatcgtccc cggtgacgcc tgtggccatg
      541 tagccctcga atgcatcacg gagcgacacg gccggcggga ccacgtactg ccccggagac
      601 gcggtaagtc gcgcgactct cttgagcggc gccgacgggt cgcacaggcc gatctgcgcg
      661 ggcgaagtca ccagaccatc accgggctgc ccgtcgctgg tgttgatctg atgccgcgga
      721 gggctctcga tggcgaccac atcatcgaac cacgcggtgc cggaagcgcg atagatccca
      781 cactgcagtt caacgcgtgt cgcgccaccc gccgcccgga agccgtaagt gtggcgagcc
      841 cagtcggttg tcccgcgcac atggcagatg tcatggaaat ccaccagctt gccgtcctcg
      901 ccatactggt agacggcgag gtacgcgaag ccgtcgccct caatggatgc cgtgcggagc
      961 caacccacaa ccaggtagtc ttgcccggtc ttgggcgtga atgagcagcg ccacatcgaa
     1021 tgccccgcgg gctgaccggg ctccatctgg actcgagcac acctctggcc cgaccgggcc
     1081 ccctgtgcga cacgtgttgc gttgccgtcg gcggcctccc aggtcgagcg cgcgtcctgc
     1141 tcaaacccgg ggtcgggcac caggccaccc tgtccggcca ggccctcctg aagccgtctg
     1201 tcccgccact cggacgcggc gatccagccg gccccatctc tcacgagcgg gcgctggaat
     1261 gggtagccgc ctgtggtcag cagatcaccc ccaccatgga ggaaggcgac caggttgcgt
     1321 cgggcctcgg cggggaaggt ctggcccgtc ggcaccacca gcagggcctg ccggtccggc
     1381 gagagctggc cggggctgct gagttgatct gccgtgaggg cttctacctc gatcccggcc
     1441 gcctgtagca gctcggccag atacgcggga tccgacggtg cgccataggt gggaaatgca
     1501 ggatcagtca gaatcgccac cctggggggc aacccgggtt gctcccgcgc cacctcgacc
     1561 gggtcggcag catccatacc cggggtcagc acggcgtcgt cgaagtaggc gacacccgaa
     1621 gcattgaaaa agcccaggcg catggagacg atgtccgcca gtgggttggc gcggaacgag
     1681 aagctctgcc gcgtccagcc tcccggttcc gtgagttgag cgaaatcacg gaactccaca
     1741 agagcgccat cgaggctgta ctggtagacc gcggcgaacg cgaaaccacc gggagcactt
     1801 ggcgtcacct gctcgacctt catccacacc gagcaggtgt aatcaccccc tggcgcagcg
     1861 agcacgacgt cttgggagca gacgccccgt tcgacacgga ggcagcgccc cctgcgcccg
     1921 gcatccgaga ccgcgctgcc gaccgagagc gaccacccct cagtcacctc cagcgtgccg
     1981 ttgcgcagga attggagcgg ctgcgcgccc gccgcggagc caacagcggc aagacagagg
     2041 aggacgaagg gattcaagga agagaccccc tgaagcccgc cggatcgtgt ccgcgggcag
     2101 tctggtggac gagttcgtcc catgcgttgg atccccctgg cccaggcagc gagggagttc
     2161 cggccgaacg gggcggaatg gagtgccgat gactcgagac gagttgctgg ccatttacag
     2221 ccgcatcccc accgagtcca cgtgccgcga ctgccacgaa tgcgccattc gctgtagcgg
     2281 ggcgatcccg ttggcccgaa gcgaatggga ggggatcgcg gcctatatcg agacacatat
     2341 gtccgatctc gagttcgaga gcctcctggc gcaggacaag cgcttcgagg tcgctgatgg
     2401 agtctgggag gagttctgta tactctatga cgtggtcgcc ctgcgctgcg ccgtgtaccc
     2461 ggttcggcca ctcgtgtgcc ggctgctggg gcacgtcgag ttcatgccat gccccctcag
     2521 ccgtgtgccc agcgtcctgc cggatggccc cgagatcatg aggcgctatg cccagctcga
     2581 gcggaagact gtggcggagt ggctggcggg ggaaccccgc cgtggctgtg ggccgagagg
     2641 agcctgagcg tggctgtttt gaccgtggaa cgccgcattg gtgcccccat cgaccgcgtc
     2701 tacgccttgg cgcgcgacgt ggagcgattc ccggagatca tgccggatgt cgagagcgtg
     2761 gagatcctct cgcgcgacgg gagccacacc acgtcgcgct gggtcggcgt agttcgccag
     2821 ttcaaccgcc gcttggtgtg ggtggcctac atcggcggca agcgcgagag ccgtcggttg
     2881 ctgggtgatg tgatcctgca gcagcaggat attgaaacag gcaaagcata tcccgatgcc
     2941 tgtgtcacca cgacgtgggg gattgacctt cactatccgc atccagagaa cagccgccac
     3001 ttccccggga tggaattccg tgccgtctct aaaaccaaga gcatcaagcc gtacgcgatt
     3061 ccataccgtt gcctttacag ccgctcgctg gacaatttgt tcatggcggg gcgcaacatc
     3121 agcgtcaccc atgtggcgct cggctcggtg cgcgtgatgc gcacgggcgg catgatgggg
     3181 gaggtggtcg gcatgtcggc tgcgctctgc acgcgccacg gcaccacgcc acgcggtgtg
     3241 ttcgaagctc acctgcctgc gttgcaggaa cagatgcggc gcggcgtcgg gcggtttcgt
     3301 tagttgatta gttaattagt tgattagttg attagttgat tttcctccct taa
//
LOCUS       DAOSPL010000355         3352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28480_length_3352_cov_3.125265, whole genome shotgun sequence.
ACCESSION   DAOSPL010000355 DAOSPL010000000
VERSION     DAOSPL010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28480_length_3352_cov_3.125265"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..219
                     /gene="rpmD"
                     /locus_tag="PLQ54_07735"
     CDS             40..219
                     /gene="rpmD"
                     /locus_tag="PLQ54_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOS93188.1"
                     /translation="MGRIRVTLKKSPIGHEKDQRETARLLGLRRLNASVVRPDTPVVR
                     GMVRKIRHLVEVEEA"
     gene            250..706
                     /gene="rplO"
                     /locus_tag="PLQ54_07740"
                     /pseudo
     CDS             250..706
                     /gene="rplO"
                     /locus_tag="PLQ54_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896438.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            709..2043
                     /gene="secY"
                     /locus_tag="PLQ54_07745"
     CDS             709..2043
                     /gene="secY"
                     /locus_tag="PLQ54_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOS93189.1"
                     /translation="MARAQVTQTSKMRQTLVDAVSLPDVRKRILFVGAMFAVYVGCIH
                     IPVPGIRHDALENIFGGAGGSNILDLMNAFAGGALKRMSVIGLGIMPYINASIIFQLL
                     TIAVPSLERLQRDEGDYGRKLIAKYVRWLTVFLAFVQAVGLITVLRGMNAVEPGVGLG
                     FLMVISLAAGTSFLMWIAEELTDKGIGNGVSLVIFCGIMCNMPQEIGRTLAQARAGAI
                     NVPQLLLLAICLVMIIALIIFVQQGQRRLRVQYAKRVVGHRVYGGHSSFLPLKVNAAG
                     VIPIIFAISIFMFPATVVQYVPAQSEWWRVWASRITSFHPGVDVVGIAAALVYFVLVV
                     IFTYFYTAVTFKPTEIADSMKKNGGFIPGIRPGKPTAEYLDRLMQRITLPGALFLGLI
                     ALTPYYLPALTKVTTFSLVGGTSLLIVVGVAMDTMQQMEAHLLMRNYEGFLR"
     gene            2049..2699
                     /locus_tag="PLQ54_07750"
     CDS             2049..2699
                     /locus_tag="PLQ54_07750"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015590328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOS93190.1"
                     /translation="MDIILLGPPGSGKGTQGVEIASKLGIPKLSTGDLLREAVAAGTE
                     LGRQAEPIMKSGGLVPDEIVIGMVRERAVSPECAKGMLLDGFPRTLPQAEGLDAMLSS
                     LGRSVTAVIDVMVPDDEIVGRLSGRWSCPVCGAVYHVTANPPKRQGRCDQDDAELRQR
                     ADDRAESVRERLRVYHAQTKPLAEVYQSRGLLRQVDGLGPIGEVRERVAQAIADLA"
     gene            2738..>3352
                     /gene="map"
                     /locus_tag="PLQ54_07755"
     CDS             2738..>3352
                     /gene="map"
                     /locus_tag="PLQ54_07755"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOS93191.1"
                     /translation="MTGQAGRRPAEIPLKTPEQIEIMREGGRILARALAAAGEMIEPG
                     VTTAALDRRVAEVLRESGAQASFLGLYGFPASVCVAVNDVVVHGIPHEEVRLRDGDII
                     GIDLGVFWGGLHVDGASTYEVGAVGRAVRKLLTTTRTALNLGIAAARPGARIRDISRA
                     IQDHVERRRYSCVRALCGHGIGFEVHEAPHVPNFVSREAGPVIVP"
BASE COUNT          578 a          991 c         1190 g          593 t
ORIGIN      
        1 acgggaaagc gagatcggac aatggcagat aacgaggccg tgggccggat cagagtgacc
       61 ctgaagaaga gccccatcgg tcatgagaag gaccagcggg agaccgcccg gctcttgggg
      121 cttcgacggt tgaacgccag cgtggtgcga ccagacacac cggtggtgcg gggcatggtc
      181 cgcaagatca ggcatctggt cgaggttgag gaagcctagt cggccgacac gagggggcga
      241 cacgtggtgt tgagtctcga tagtattagc cctctgccgg gcgcgcggaa gaagcggacc
      301 cgcgtgggcc gcggccatgc ggcgcaaggg caagacctgc ggccgcggca tgaagggcca
      361 gaaggcgcgc aacaaggtcc gccgcggctt cgagggcggc cagacgccac tgcagcggcg
      421 gctccccatg cgccgaggcg tcagtaataa gtccatgaac attgggatct tccgcaagga
      481 gttcgccgag gtcaacgtcg gcgtcctcga cgccgccttc gaggccgatg ccgagatcac
      541 tccccaggtc ctgctggagc ggcggatcat ccggcagttg cgggatggcg tgaaggtcct
      601 gggtgtcggc cgtgtgacca aaaggctgat cgtgaaggcc aacgccttct cccaatccgc
      661 ccgagcgaag gtcgaagccg ccggaggcgt ggcggaggag gtctagcgat ggcgagggca
      721 caggtcaccc agacgagcaa gatgcggcag acgctggtcg acgcggtctc gctgccggac
      781 gtgcggaagc ggatcctgtt cgtcggggcc atgttcgcgg tctacgtcgg gtgtatccac
      841 attccggtgc ccggcatccg gcacgacgca ttggagaaca tcttcggcgg cgccggtggg
      901 agtaacatcc tggacctgat gaacgccttc gccggtggcg cgctgaagcg gatgtcggtc
      961 atcggcctgg gcatcatgcc ctacatcaat gcttcgatca tcttccagct gctgaccata
     1021 gctgtgccga gtctcgagag gctccagcga gacgagggcg actacggacg caagctgatt
     1081 gcaaagtacg tgcgctggct cacggtgttc ctggccttcg ttcaggctgt tggcctgatc
     1141 accgtgctgc ggggcatgaa tgccgttgag cccggcgtgg ggctggggtt cttgatggtc
     1201 atctcactgg cggcgggcac gtcgttcctg atgtggatcg ccgaggagct gaccgacaaa
     1261 ggcatcggca acggggtgtc actggtgatc ttctgcggca tcatgtgcaa catgccccag
     1321 gagatcggac gcactctggc gcaggcgcgc gcgggggcca tcaatgtgcc gcagctgctg
     1381 ctgttggcca tctgtctggt catgatcatc gcgctgatca tcttcgtcca gcaaggccag
     1441 cgccggctgc gggttcagta tgccaagcgc gtggttgggc accgggtcta cggcgggcat
     1501 tcgagcttcc tgccgctgaa ggtcaatgca gccggcgtca ttccgatcat cttcgccatc
     1561 tcgatcttca tgttccccgc cacagtggtt cagtacgttc ccgctcagag cgagtggtgg
     1621 cgtgtctggg ccagccggat cacgtcgttc catccgggtg tcgatgtggt gggcatcgcg
     1681 gcggccttgg tctacttcgt gctcgtcgtc atattcacgt acttctacac tgcggtcacg
     1741 ttcaagccca ctgagatcgc ggacagcatg aagaagaatg gcgggttcat accagggatt
     1801 cgacccggca aaccgacggc cgagtatctg gaccgcctga tgcagcggat cacactgccg
     1861 ggcgccttgt tcctgggcct catcgccctg acgccgtact acctaccggc cctgaccaag
     1921 gtgacgacat tcagcctggt gggcggcacc tcactgctga tcgtggtggg cgtcgccatg
     1981 gacacgatgc agcagatgga ggcgcatctg ctgatgcgga actacgaagg gttcctgcgc
     2041 taatggccat cgacatcatt ctgctggggc ccccgggttc ggggaagggc acacagggcg
     2101 tggagatcgc ctcaaagttg ggtattccca aactctccac aggcgacctc ttgcgcgagg
     2161 ccgtcgccgc cggcaccgag ctgggccggc aagccgagcc gatcatgaag tcgggtgggt
     2221 tggtgccgga cgagattgtc atcggcatgg ttcgggagcg cgcggtttcg cccgagtgcg
     2281 ccaagggaat gctgctcgat gggttcccgc ggaccctgcc ccaggccgag gggctcgatg
     2341 ccatgctgtc gagcctgggg cgaagtgtca cggcagtcat tgacgtgatg gttcccgacg
     2401 acgagattgt gggacggctg tccggacgct ggtcgtgccc ggtctgtggg gccgtctacc
     2461 atgtcacggc aaaccccccg aagcggcagg ggcggtgcga ccaggacgac gcggagctcc
     2521 gacagcgcgc cgatgaccgc gcagagtcgg ttcgcgagcg gttgcgggtg taccacgcgc
     2581 agacgaaacc gttggctgag gtataccagt cccgaggtct gctccggcag gtggacggcc
     2641 tgggccccat cggggaggtc cgcgagcgcg tcgctcaggc gatcgcggac ttggcctaga
     2701 ggcgtgaggc ggccaaggcc tcgggaagtg ggccgaagtg accgggcagg caggccggcg
     2761 accggcggag atcccgctga agacgccgga gcagatcgag atcatgcgcg agggcggccg
     2821 gatcctggcc cgggccctgg cagcggccgg cgagatgatt gagccaggag tcacgacggc
     2881 tgcactggac cggcgggtgg ccgaggtgct tcgcgagtcg ggcgctcagg cctcgttcct
     2941 gggcctctat ggcttcccgg ccagcgtttg cgttgcggtc aacgatgtgg tagtacatgg
     3001 aattccccac gaggaggtcc gcctccgcga tggggacatc attgggatcg acctgggagt
     3061 cttctggggc ggcctccatg tggacggtgc gtcgacctat gaggtcggcg cggtgggcag
     3121 agcggttagg aaactgctca ccacgacacg gacagctctg aacctgggaa tcgcggcagc
     3181 acgtccgggg gcccggatcc gggatatatc acgggccatc caggatcacg tcgagaggcg
     3241 acgctattcg tgcgtgcggg cgctgtgcgg ccacgggatc gggtttgagg tccacgaggc
     3301 accccatgtg cccaactttg tctcacgcga ggcgggtccg gtcatcgtgc cg
//
LOCUS       DAOSPL010000356         3350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28500_length_3350_cov_4.168134, whole genome shotgun sequence.
ACCESSION   DAOSPL010000356 DAOSPL010000000
VERSION     DAOSPL010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28500_length_3350_cov_4.168134"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="PLQ54_07760"
     CDS             complement(<1..814)
                     /locus_tag="PLQ54_07760"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOS93192.1"
                     /translation="MMRALARPDHAFAAGLLFLAMACVGGCGGDGSGGGNPDVVGPGN
                     TTIQGTVVDSANTGNVVGNAYVYVPPRVRGEAGSRTTVVAATHSAANGTYTLQGVPEG
                     NQTIMVVPPDGSNYAQEALDIQVPASGTVNLRLTLTDRTVALGVSEVRVTPSPAALNV
                     GGQQQFTATVIGPNSQVLALTPTWIVTGNIGTVDQNGLFTATSAGSGQVTAVVAGVTG
                     SADVTATASLIADAGLEAAIREHLGNPAGSLTAAQLQSMTGTLNASDRGIRSL"
     gene            1042..2325
                     /locus_tag="PLQ54_07765"
     CDS             1042..2325
                     /locus_tag="PLQ54_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOS93193.1"
                     /translation="MVRPKPTVGDTSWFVRDRFGLFIHWGLYALPARHEWVKHVEQIP
                     DEQYDVYFRHFDPDLYDPRLWAEAAAGAGMKYFVVTTKHHDGFCLWDSQLTDYKAPNT
                     PCGKDLIRPMVEAFRERGLRVGFYHSVIDWHHPDFVVDNLHSMRGRPDREALNATRDQ
                     TRYAAYLHGQVRELLTGYGKIDVLFYDFSYPPEKGRNDWNSLELLKMTRELQPGIIVN
                     DRLDLQDVEGGWDIRTPEQFMPREWVQVDGQPVVWETCQTFSGSWGYHRDEASWKSVE
                     QLVQMLIGVVSRGGNLLLNVGPTGRGEFDDRALERLRGMGEWMKRHSRSIYGCTQAPD
                     GIPCPQDCRLTYNPETNRLYVHIFAWPFVQLHIDGLAGKVAYAQLLNDASEVKMGEVP
                     EHQASMAGPSPDTLTLHLPVRKPDVVVPVVELFLK"
     gene            2536..3213
                     /locus_tag="PLQ54_07770"
     CDS             2536..3213
                     /locus_tag="PLQ54_07770"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS93194.1"
                     /translation="MRNRGFTLIELLVVMAIIAILASILFPVFAKAREKARSISCLSN
                     IKQLSMATAIYMSDWDQRFPMLRYYPVGGYSYRFFDGQGTIRCYFWVEALQPCLRNYE
                     IFQCPSRDVSGNRDGSATFRSMSFRHAYALNAYLDGGSEGSIQDASGTIMYCETDWPC
                     PDLGLWSTNGIPVRNGEGIHAGMVNWAFCDGHTKAMKLSSTLRPSASGPNMWVWYDTG
                     VQPPVVW"
BASE COUNT          556 a         1107 c         1063 g          624 t
ORIGIN      
        1 tcaggcttcg gatgccccgg tcgctggcgt taagcgtccc ggtcatgctc tgcagttggg
       61 ccgccgtcag actgccggca gggttgccca ggtgctcgcg gatggctgct tcgagccctg
      121 catctgcgat gagcgacgcc gtagcggtaa catccgccga gcccgtcacg cccgccacca
      181 cggccgtgac ttgcccgctc ccggcgctcg tcgcggtgaa caggccattc tggtccaccg
      241 tgccgatatt gccggtcacg atccacgtgg gagtgagggc gagtacctgc gagttgggac
      301 caatcacggt tgcggtgaac tgctgctgcc cgcccacgtt cagtgcagca gggctgggag
      361 taacccggac ctcggacacg ccgagggcca cggtccggtc cgtcaaggtc agcctcaggt
      421 tcaccgttcc cgaggcgggc acttgaatgt ccagtgcctc ctgtgcgtag ttggacccgt
      481 cgggggggac gaccatgatg gtctggttgc cttccggcac gccctggagg gtgtaggtgc
      541 cgttggccgc cgagtgggtc gcggccacta cagtcgtacg ggaccccgcc tcgccacgca
      601 ctcgtggcgg cacatagaca tacgcgttcc ctacgacatt gccggtgttg gccgagtcga
      661 ccacggtccc ctggatcgtg gtgttgcccg gtccaaccac atcggggtta ccgcctccgc
      721 tgccgtcgcc accgcagccg ccaacgcagg ccatagcgag aaacaggagt ccggccgcaa
      781 acgcatggtc tggtcgagcc agtgcgcgca tcatgcgtct ctcctccttc gccctccact
      841 gacccattcg ccagtccgtc ctccattccc tatgccggag gatacggccg ccgtcgttcg
      901 gccgacgaag gcgctggtgg cggttggcgc gaatgggtca gcgttccgtt cgcgatggtc
      961 accgggccct gggcggcgcc gtctctgcat cctgaggggc ctcgctggcg ccgcggcggt
     1021 gaactgagag gagcctgtgc catggtgcgg cccaaaccca cggtcggcga taccagttgg
     1081 ttcgtccgcg accgattcgg cctgttcatc cactggggac tgtacgcact gccggctcgg
     1141 cacgagtggg tcaagcatgt cgagcagatc cccgacgagc agtacgacgt ctacttccgt
     1201 cactttgacc ccgacctcta tgacccacgc ctgtgggctg aggctgcggc aggtgccgga
     1261 atgaaatact tcgtggtgac caccaagcat cacgatgggt tctgcctgtg ggactcccag
     1321 ctcaccgact acaaggctcc gaacacgccg tgcgggaaag acctgattcg ccccatggtc
     1381 gaggcctttc gtgagcgggg cctgcgtgtc gggttctacc attcggtcat cgactggcac
     1441 catcccgact tcgtcgtcga caacctgcac tcgatgcgcg gccgcccgga ccgcgaggcg
     1501 ctcaatgcaa cgcgcgatca gacccgctat gcggcctacc tccatggcca ggtgcgcgag
     1561 ttgctcaccg gctatggcaa gatcgacgtg cttttctacg acttcagcta tcctccggag
     1621 aaaggccgca acgactggaa cagcctcgag ctcctcaaga tgactcggga gctccagccc
     1681 ggcatcatcg tcaatgatcg cctcgacctc caggacgtcg agggggggtg ggatatccgc
     1741 acgcccgagc agttcatgcc ccgcgaatgg gtccaggtgg atggtcaacc cgtcgtgtgg
     1801 gagacctgcc agaccttctc cggctcctgg ggctatcacc gtgacgaggc ctcctggaag
     1861 agcgtcgagc agctggttca gatgctgatc ggggtcgtga gccgcggcgg caacctgctg
     1921 ctgaatgtcg gcccaaccgg ccggggcgag ttcgacgacc gagctctgga gcgcctccgc
     1981 ggcatggggg agtggatgaa gcgccacagc cgctccatct acggctgcac ccaggcaccg
     2041 gatggtattc catgcccaca ggattgccgg ctcacctaca atcctgagac caaccggctc
     2101 tacgttcaca tcttcgcctg gccgtttgtc caactgcaca ttgacggact ggccggcaag
     2161 gtggcgtacg cccagctcct gaatgatgcg tctgaggtga agatgggtga ggtgcccgag
     2221 catcaggcga gcatggccgg cccctcgccg gacacactga ccctgcatct gcccgtacgc
     2281 aagcccgacg tagtcgtgcc cgtcgttgag cttttcctga aatagaaccc aactggacga
     2341 gctgcaggga cgtcatactg ggtggtgggc gggccgcgcg gcgcagtcgc cggcacacat
     2401 gccgctggcg cgctagactg gtactgggac ccgttgcggc cgcgaaaccc cactcgaacc
     2461 ggcatccggt ccgagcacgg gacatacgta atgggtctcg gcgggctgtg gcccgcctag
     2521 ccggaggtgg cgcacgtgag gaaccgcgga ttcaccctga tcgagctact ggtcgtcatg
     2581 gcaatcatcg cgatcctggc gtcgatcctg ttccccgtgt tcgccaaagc acgggagaag
     2641 gcccggtcca tcagttgcct ttccaacatc aagcagctca gcatggccac agccatctac
     2701 atgagtgact gggatcagcg gttccccatg ctgcgttact acccggtggg cggatactcg
     2761 taccggttct tcgacggaca gggaaccatt cgctgctact tctgggtcga ggcgctgcag
     2821 ccgtgtctgc gcaactatga gattttccag tgtccttcgc gcgacgtgtc cggcaatcgc
     2881 gatgggtcgg ccacgttccg ctcgatgtcc ttccgtcacg cctacgcgct caacgcctac
     2941 ctggacggcg gatcggaggg ttcgatccag gatgcgtccg gcaccatcat gtactgtgag
     3001 acggactggc cgtgtcccga tcttggcctc tggtccacca acgggatccc ggtgcgcaat
     3061 ggtgagggga tccatgcggg gatggtcaac tgggcgttct gcgacgggca caccaaagcg
     3121 atgaaactca gctcgacact gcgtccctcg gcctcgggcc ccaacatgtg ggtgtggtat
     3181 gacaccgggg tgcagccacc cgtggtgtgg tgaaccgcgg gcgcgacgtg gctacggcgc
     3241 gcccgcgccc aaccccagct ggctctcgat ccctcgcatg gcctcgatga cgttctcgat
     3301 gtgctcgtcc accggcacgc ccagcactgc ggctccggct tcgatggctt
//
LOCUS       DAOSPL010000357         3345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28560_length_3345_cov_3.057143, whole genome shotgun sequence.
ACCESSION   DAOSPL010000357 DAOSPL010000000
VERSION     DAOSPL010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28560_length_3345_cov_3.057143"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2793
                     /locus_tag="PLQ54_07775"
     CDS             <1..2793
                     /locus_tag="PLQ54_07775"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOS93195.1"
                     /translation="VRDLNGVGKTIRSAIKKAKSHGWTPDEFRDRMLAACDRFWQALP
                     SPDNPPAPDDVFEEVRNGLCRAGLSRTGPDAGDKGNEVRKLYHGTASPPITSEALRER
                     FEREARSERTAVRLCAAAYRVYQAGLEEQGLLDFDDQILRTVRLLSENDDLRQEYRAR
                     FEYVLVDEFQDTSPVQMELIRLLARPEPCATPCERRSTCGGLRLTNVMIVGDKKQSIY
                     AFRNARKENLDDLIPCTGRDVVHELTRNYRSGPAVIQVANIVARAVEPADPDLICEVS
                     HDAIVVRGPDFPSGGGISAEAARDAESAYIAERIRALVDGGEFAYGDIAVLVRGKTRF
                     PSLKRAFDARRIPYVSLGGLGFFSEPLVRDALATLRLISDPCDDLSLIRLLTRPPVGL
                     TDRQLFLLHAWEPREQAEEGVPRPALPLILRLRECLETVGPCEPELAPCLPAVRELLA
                     RLGDWAPLRHTAPARELLEGVIGCPAFLQGRSPAELVGWPTCQRILEGVLAEAEEAGD
                     DDLQGFLRRIHLYEGLGEVDVPSPDPAEPEAVRVLTAHKAKGLEFPVVFLPTAAAGNS
                     ERDAWTFDDAWGLVPSRIWGSTSLKGLLFRRLSPRSEAEEEEERRLFYVGVTRARQRL
                     FLSSTKTENHPYAELIQALADLRNETDLVLPESPRRGEALRIPAPAGRPLPLEPTEPA
                     VPRVVRTSFTQLDTMLACPVRFIIQHVWGLPELGDALGPGGQAAGSAFHHLVADFLRQ
                     PFPSAQDDAALRRALADLGIEGGTAMEALISRWQSFRQSRWATLRPDPADIERRIEWI
                     QTTPQGCRAIVTGFIDLVHRSNGAVEVVDFKTHRDLTEADRQRYAVQLSLYAWALSAE
                     LGAAEIGRRLVWVRESGVEEFETPPVDEAALVSLLEQRVEMERMRELPPKPDDAPCDW
                     CTASAMCPYGQMTDGK"
     gene            2812..>3345
                     /locus_tag="PLQ54_07780"
     CDS             2812..>3345
                     /locus_tag="PLQ54_07780"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOS93196.1"
                     /translation="MTGSGWIVPCALSVACCAIGRTVAAPLATVTGEARDVSVPITVP
                     LPMGAEEGSGELLPAAGVGAVVWQSSTRGPWAGGQPWAGAVLAPGPALDGARFAVVRG
                     KEARPAAFRFEEVDNGVVRLLENDRTVLVYHSRPTLAEGVPEQFRRAGYVHPIYGLDG
                     EVLTDDFPKDHYHHRGLY"
BASE COUNT          561 a         1100 c         1164 g          520 t
ORIGIN      
        1 gtgcgggatc tcaacggggt agggaagacc atccggtcgg cgatcaagaa ggccaagagc
       61 cacgggtgga cgcccgacga gtttcgggac cgcatgttgg ccgcgtgcga ccggttctgg
      121 caggcgctgc cgtcaccaga taaccccccg gctccggacg acgtcttcga ggaggtccgc
      181 aacgggctgt gccgtgcggg cttgtcgagg acgggccccg atgccggaga caagggcaac
      241 gaagtccgca agctctacca cggtaccgcc agcccgccga tcaccagtga ggcgctccgt
      301 gagagattcg agcgagaggc acgttcagag cggacggccg tccggctctg tgcggccgct
      361 taccgcgtct accaggccgg cctggaggag caaggcctgc tggacttcga tgaccagatc
      421 ctacgcaccg tgcggctgct ctcggagaac gatgacctgc gccaggagta ccgggcacgc
      481 ttcgaatacg tgctggtcga cgagttccag gacacctctc ccgtccagat ggagctcatt
      541 cggctgctgg ccaggccaga gccctgcgcc accccgtgcg agcggcggag cacgtgcggc
      601 gggttgcggc tcacgaacgt gatgatcgtc ggcgacaaga aacaatcgat ctacgcgttc
      661 cgcaacgccc gcaaggagaa cctggacgac ctcatcccat gcacgggccg cgatgtggtc
      721 catgagctga cgcgcaatta ccggtcggga ccggcggtca tccaagtcgc caacatcgtg
      781 gcgcgcgcgg tcgagccggc cgatccggac ctgatctgtg aggtctcgca cgacgccatc
      841 gtggtccgcg gtcccgactt cccttcgggc ggaggcatca gtgccgaagc cgcgcgcgac
      901 gccgagagcg cgtacatcgc cgaacggatc cgggccttgg ttgacggcgg cgagtttgcc
      961 tacggggaca ttgcggtgct ggtgcggggc aaaacgcgct tcccgtcact caaacgggca
     1021 ttcgatgcgc ggcgcattcc gtacgtgagc ctgggaggac tggggttctt ctcggagccg
     1081 ttggtccgcg acgctctggc cacactcagg ctcatcagcg acccgtgcga cgatctgtcg
     1141 ctcattcggc tactgactcg gccgcccgtc gggctgaccg accgccagct ctttctgctg
     1201 cacgcctggg agccccgaga gcaggccgaa gagggcgtgc ctcggccagc cctgccactg
     1261 atcctccggc tgcgtgagtg tctagagacg gtaggcccgt gcgaaccgga gctcgccccc
     1321 tgcctgccgg ccgtgcgcga gctcctggcg cgcctgggcg actgggcgcc gctgcgacac
     1381 acggcaccgg cgcgcgagct gctcgaggga gtcatcgggt gtcccgcctt cctgcagggg
     1441 agatctccag ccgagctggt cggatggccc acatgccagc ggattctcga aggcgtgttg
     1501 gccgaagccg aggaggccgg cgatgatgac ctccagggct tcctccgccg aatccacctc
     1561 tacgagggct tgggcgaggt cgacgtgccg agccctgacc ccgccgagcc cgaggcggtg
     1621 cgcgtcctga cggcccacaa agccaaagga ctcgagttcc ccgtcgtgtt cttgcccacc
     1681 gcggcggcgg gcaactctga gcgcgacgcg tggaccttcg atgatgcctg gggactcgtc
     1741 cccagcagga tctggggctc aaccagcctc aaaggactgc tgttccgccg tctctccccc
     1801 cgaagcgaag ccgaggaaga ggaggaacgg cggctgttct acgtcggtgt gacccgagcc
     1861 cggcagaggc tgttcctcag ctccaccaag acagagaacc acccgtatgc cgagctgatc
     1921 caagcactgg cggatctccg gaacgagacc gatctcgtct tgccggagtc gcccaggaga
     1981 ggggaagcac tccggatacc ggcaccggct ggccgtccgc tgcccctcga accgacagaa
     2041 cccgcagtac cccgtgtggt gcggaccagc ttcacccagc tcgataccat gctggcatgt
     2101 ccggtgagat tcatcatcca gcacgtgtgg ggcctgccgg aactcggtga cgccttgggg
     2161 ccaggcgggc aggccgccgg gtccgcgttc caccacctgg tggcggactt cctccggcag
     2221 cccttcccca gcgcccaaga cgatgcggcg ctgcggcggg cgctggcgga tctcggcatc
     2281 gagggcggga ccgcgatgga ggcgctgatc agccggtggc agtcgttccg ccagagccgg
     2341 tgggctacac ttcgccccga cccggcggac atcgagcggc ggatcgagtg gatccaaacc
     2401 acaccgcagg gatgccgcgc catagtgacg gggttcatcg acctggtaca ccggagcaac
     2461 ggagccgtcg aggttgtcga cttcaagacg caccgcgacc tgaccgaggc cgaccgccaa
     2521 cggtacgcgg tccagctctc gctgtacgcg tgggcgctct cggccgagct gggagccgcc
     2581 gagatcgggc ggcgtctggt ctgggtccga gagagtggcg tcgaggagtt cgagacgcca
     2641 ccggtggacg aggcggcact ggtgagccta ttggaacagc gcgtcgagat ggaacggatg
     2701 cgcgagctcc cgcctaagcc cgatgacgcg ccctgtgact ggtgcacggc gtcggccatg
     2761 tgcccatacg ggcagatgac agatggcaag tgacaggagc gcggtatcgc catgacggga
     2821 tccggctgga tcgtgccctg cgcactgagt gtggcctgct gtgctatcgg tcggacggtg
     2881 gctgcaccac tggcgacggt cactggggag gcgcgggatg tgtccgtccc gatcaccgtg
     2941 ccgttgccga tgggtgccga ggagggctcg ggtgaactcc tgccggccgc cggcgttggc
     3001 gccgtggtgt ggcagagcag tacgcgagga ccttgggctg ggggacagcc atgggccgga
     3061 gcggtgctgg cccctggccc ggcgctggac ggcgcgaggt tcgcggtcgt gcgcggcaaa
     3121 gaagcccgac cggccgcatt ccgattcgaa gaggtcgaca acggcgtggt ccgtctgctc
     3181 gagaacgacc ggacagtgct cgtctaccac tcgcgcccga cgttggccga aggggtaccc
     3241 gagcagttcc gccgggccgg gtacgtccac ccgatctacg gactcgatgg ggaagtgttg
     3301 accgacgatt tcccgaaaga ccattaccac caccgagggc tctac
//
LOCUS       DAOSPL010000358         3344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28567_length_3344_cov_3.284585, whole genome shotgun sequence.
ACCESSION   DAOSPL010000358 DAOSPL010000000
VERSION     DAOSPL010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28567_length_3344_cov_3.284585"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PLQ54_07785"
     CDS             <1..609
                     /locus_tag="PLQ54_07785"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOS93197.1"
                     /translation="TYEPDVVLLQEAPTAEVLDEVAARHPGYEALTRGELAIVSRGHL
                     TSVPVDPPLEDFVLAADLVAAGRRLIVASVHFSVPFRGMDFWRAETRHEARLVWEDHH
                     RQVELLAQWVRTLDPATPLIVGGDMNTAAGDSLLRDMPARLADTFRIAGIGWGNTVRN
                     RKPFLRVDYIWADTRLRPVTVVARRTEHSDHRAVVADLVWAQ"
     gene            complement(666..2021)
                     /locus_tag="PLQ54_07790"
     CDS             complement(666..2021)
                     /locus_tag="PLQ54_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS93198.1"
                     /translation="MADASVCRDRRITTGGMAAYGLGHFGASALMEAMLTLLVAFYAT
                     HDPDTGGPYLSIGAATFAVGVGRFLDAFLDPLVGYWTDRIRTRWGRRAPVLAATLPPM
                     CLFFALMWLPLTPSPSVANLLALTASATLFVMAFTAYMVPYLAWLPDLARSVGDRTRA
                     MGWMAFFNVLGVALGSTGAGQLAAVPALGFGGMGLVLAGVTLATGGVTLATGLHRLEQ
                     AAPERPALPIVAAVRTALANPHFRHYLPSFALFWVGLRLLLYSLPFFIMTVFEVPKGQ
                     AAAAMGTLMGCAIGVGALLFPVIPRLAVRYGKARLYRAGLGLFCLTAPLLPLVGLVGD
                     AGTGLHQAMVLMAVAGIPTGIMFVIPYTLLADVIDYDEDQTGTRREALYFGVQGFFVK
                     VAQLAGLGLGGLLVGGLGTTSASGPRLAGPMAAVFVAAGLAWFRTYALQPRDAPTADE
                     R"
     gene            2144..2509
                     /locus_tag="PLQ54_07795"
     CDS             2144..2509
                     /locus_tag="PLQ54_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93199.1"
                     /translation="MSYSPQAPGAMPPQWTPSHVPTSDANRPLIIALIVVGCLLLLAI
                     GGCMVLGFHLARQAQRATESITQTMVPLMETQTEQEIQRLERRLDATSAGSPERADLE
                     RQLRAMRELRDSLKERPTR"
     gene            complement(2550..>3344)
                     /locus_tag="PLQ54_07800"
     CDS             complement(2550..>3344)
                     /locus_tag="PLQ54_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOS93200.1"
                     /translation="QEAVDAARALGLDVGAGAFGENLVSSGLDTEALGLGSRIRVGVS
                     AELELTQRGKICHDPCAIQEITGECIMPKAGLFFTAVQGGPVRPGDAIEIRALVPREL
                     FQVGLVTVSDRSASGERPDETGPALKQMVEEALQSRVADARVVPDDIDQICQALIDLC
                     ERGLDLILTAGGTGFAPRDVTPEATRQVVERLAPGLDEAMRAASLRVTPRAMLSRGIS
                     GTRGATLIVNLPGSPKGSRENLGVLIPVLPHALGLLRGTDRDCGGR"
BASE COUNT          551 a         1203 c         1121 g          469 t
ORIGIN      
        1 acctacgagc ccgacgtggt gctcttgcag gaggcgccga cagcggaggt cctcgacgag
       61 gtcgcggcgc gccacccggg ctacgaggcc ctcacgcgtg gggagctcgc catcgtgtcc
      121 cgtggccacc tgacgtccgt tccggtcgat ccgccgctcg aggacttcgt cctggcggcg
      181 gacctggtcg ccgccggccg tcggctgatc gtagccagcg ttcatttcag tgtgcccttc
      241 cggggcatgg acttctggcg ggcggagacg cgccacgagg cacgcctggt gtgggaggac
      301 catcaccggc aggtcgaact gctggcccag tgggtccgca ccctcgaccc cgccaccccc
      361 ctgatcgtgg gcggcgacat gaacactgcg gccggcgaca gcctcctccg cgacatgccg
      421 gccaggctgg ccgatacctt ccgcatcgcc ggcatcggct ggggcaacac cgtccggaac
      481 cgcaagccgt tcttgcgggt ggactacatc tgggcggaca cgcgccttcg tccggtaacc
      541 gtcgtggcgc ggcgaaccga acactcggac caccgtgccg ttgtcgccga cctcgtatgg
      601 gcccagtgac cggccaggcg ggcgccgcat ccgtttcgcg ccgccggatc ccccctgaga
      661 cggggctagc gctcgtcggc cgtcggcgca tcccggggct gcagcgcata ggtgcggaac
      721 cacgcgagcc ctgcggccac gaacacagcc gccatcgggc ccgccaggcg cggacccgat
      781 gcactcgtgg tccccagtcc acccacgagc aggcccccga ggccgagtcc ggccagttgg
      841 gcgaccttca cgaagaaccc ctgcacgccg aagtagagtg cctcgcgccg cgtccccgtc
      901 tgatcttcgt cgtaatcgat cacgtcggcc agaagcgtgt acgggatgac gaacatgatg
      961 cccgttggga tgcccgccac ggccatgagc accatggctt ggtgaagccc tgtgccggcg
     1021 tcccccacca ggccaacgag cggcagcagg ggggcagtga ggcagaaaag ccctagcccc
     1081 gcccggtaca gccgtgcttt gccatatcgg accgcgagcc gagggatcac cgggaacaga
     1141 agcgctccga ccccgatcgc gcagcccatg agggtcccca tggccgccgc ggcctgtccc
     1201 ttgggcacct cgaacacggt catgatgaag aacggcagcg agtagagcag cagtcgcagg
     1261 ccgacccaga acagcgcgaa gctcggcaag tagtgccgga agtgggggtt cgccagggcc
     1321 gtgcggaccg cggcgacaat cggcagggcc gggcgttcgg gcgcggcctg ttcgagtcgg
     1381 tgtaggcccg tcgcaagtgt gacgcctccg gttgcgagcg tgacacccgc caagaccagc
     1441 cccatacccc cgaaccccag cgcgggcacc gcagcaagct gtcccgcgcc cgtgctcccg
     1501 agcgcgaccc cgagcacgtt gaagaaggcc atccagccca tcgccctcgt gcgatcgccg
     1561 accgatcgag ccaagtcggg cagccaggcg agatagggca ccatgtaggc cgtgaaggcc
     1621 atgacgaata gcgtggctga ggccgtcaag gccagcaggt tcgccacgga tggcgacggt
     1681 gtgagcggca gccacatcag cgcgaagaac aggcacatgg ggggcagtgt ggccgcgagc
     1741 acgggggccc ggcggcccca acgggtgcgg atccggtccg tccagtaccc gaccagtggg
     1801 tcgaggaacg catcgaggaa gcgtccgacc cccacggcga aggtggcggc gccgatgctg
     1861 aggtagggcc cgccggtgtc ggggtcatgt gtggcgtaga acgccacgag cagtgtcagc
     1921 atggcttcca tgagcgcaga ggccccaaag tgccccagcc cataggcagc catgccgccc
     1981 gttgtgatcc gccgatcccg gcacacgctg gcgtcggcca tagcccctcg gagttcggcc
     2041 cgcgccggtc cggacctgcc tccggcgagg ccctcgggtc attccgcgcg aactcttgag
     2101 cccacggagc gcaccaaagg ccgtgcgacg ggagggactc atcgtgagct actcaccgca
     2161 ggcccccggg gccatgccgc cgcagtggac accgtcccat gtgcctacct ctgatgccaa
     2221 ccgtccactc atcatcgcgc tcattgtggt gggctgccta ctcctgttgg ccatcggcgg
     2281 gtgtatggtc cttggcttcc atctggcgcg ccaggcccaa agggcaactg agagcatcac
     2341 ccagaccatg gtgcccctga tggagacgca gaccgagcag gagatacagc gcctggagcg
     2401 gcggctcgat gccacgtcgg ctggttctcc cgagcgcgca gacctcgagc ggcagttgcg
     2461 cgccatgcgc gagttgcggg actcgctcaa ggagcgtccg acgcggtgag ccccctccgc
     2521 gggcggccgc gtgcggagcc cgctgaccct catcggcccc cgcagtccct gtcggtcccc
     2581 cggagcaggc ccaatgcatg gggcagaacg ggaatcagca cacccaggtt ctcgcgcgag
     2641 cccttggggc tcccgggtag attcacgatg agcgtcgccc cgcgtgtgcc ggagatgccg
     2701 cgggacagca tggccctcgg ggtcacccgt agactggcgg cccgcatggc ctcatcgagt
     2761 cccggggcca agcgttcgac cacctgtcgg gtggcctcgg gcgtcacgtc ccggggcgcg
     2821 aaacccgtgc cgcctgccgt caggatcaga tccagccccc gctcgcacag atcgatcagg
     2881 gcctggcaga tctgatcgat gtcatcaggc acaacccgcg cgtcggccac ccgcgactgg
     2941 agcgcttctt cgaccatctg cttcagagcc ggccccgtct cgtcaggccg ctcgcccgaa
     3001 gcgctccggt cgctcaccgt gaccaggccc acctgaaaca actcgcgcgg aaccagtgcc
     3061 cgaatctcga tcgcatcgcc ggggcgcacg gggccgccct ggactgccgt gaagaacaac
     3121 cccgccttgg gcatgatgca ctcgccggtg atctcctgga tggcgcaggg gtcgtggcag
     3181 atcttgcctc gctgagtcag ctcgagctca gcggagaccc ccacgcggat ccgggacccg
     3241 agccccaggg cttcggtgtc cagaccggag gagaccaggt tttcgccgaa cgcgcccgcg
     3301 cccacgtcca ggcccagcgc gcgcgccgcg tcgactgcct cctg
//
LOCUS       DAOSPL010000359         3342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28598_length_3342_cov_3.016124, whole genome shotgun sequence.
ACCESSION   DAOSPL010000359 DAOSPL010000000
VERSION     DAOSPL010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28598_length_3342_cov_3.016124"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            293..751
                     /locus_tag="PLQ54_07805"
     CDS             293..751
                     /locus_tag="PLQ54_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOS93201.1"
                     /translation="MRITERSHYGLRAMICLAQHSPGRPMSAREISAREGIPPRYLEQ
                     VLNRLKNGRLIMGHRGPGGGYVLARDASEISARDILAVVEDSDGGRGGFLAEIECAAA
                     RGPDGAKVCGPGCLSKRVWVTLAAKVDEVLSQFRLADLVAGAGGAEDVGE"
     gene            748..1917
                     /gene="nifS"
                     /locus_tag="PLQ54_07810"
     CDS             748..1917
                     /gene="nifS"
                     /locus_tag="PLQ54_07810"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOS93202.1"
                     /translation="MKTIYLDHSATTPVDPEVVQAMLPFFTERYGNASSIHHAGRDAY
                     DAMGAARESVASLIGASPDEIYFTGGGTESDNLAIQGYAGHPVNRKRGKHIITSKIEH
                     HAVLHTCQFLERQRGHQVTYCGVDGNGVIDLDELAASIADDTILISVMLANNEVGTIE
                     PLREIRRIVGDRPIAIHTDAVQALGEMDIDVNELGVDMMTLSAHKVYGPKGVGALYIR
                     RGTRVGPIMHGGGHERRKRPGTENVPGIVGFGKACELLARDGAAVRERVARLRDRLVE
                     GVMARVDDVVLNGHPTRRIPRNASLCFRGCEGEALLLHLDMAGICASSGSACTSQSLE
                     PSHVLAAMGVPIEIAHGVLRLTLGKGTTEEEIDYTVDTLTSVVDKIRGISAALGV"
     gene            1984..2361
                     /gene="nifU"
                     /locus_tag="PLQ54_07815"
     CDS             1984..2361
                     /gene="nifU"
                     /locus_tag="PLQ54_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HOS93203.1"
                     /translation="MYTEKVMEHIASPRNVGVLEDADGVGTVGNPVCGDMMKIYIKVN
                     GDRIDDIRFQTFGCGAAIATSSILTELAKGKSLDEAMAITRDTVARELGGLPAQKMHC
                     SNLAADGLHEAIRDYQARREGQE"
     gene            2514..>3342
                     /gene="hrcA"
                     /locus_tag="PLQ54_07820"
     CDS             2514..>3342
                     /gene="hrcA"
                     /locus_tag="PLQ54_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOS93204.1"
                     /translation="MRLELDERRRRVLAAIVRDYVSTAEPVGSKRLVERHLFRVSSAT
                     IRNDMSALEAVGLLAQPHTSAGRIPSDRGYRFFVDELMEIPVLSPAAERILRSAREHT
                     TDIDDMLREICTILSKVTQHTAVALVPGLEKAELRHFQISKVDGARCLIILVTDAGHV
                     VHEFIDAGESYSAREIEVMSGILRRRLVGMKIDDIEALDTRHLRAEPQLRGLLLEESL
                     RVVRDHLERGSYWVFVDGAMHIVTQPEFNDVERLQRVLHALEQEQLLAQLLSECLRTQ
                     "
BASE COUNT          573 a         1014 c         1212 g          543 t
ORIGIN      
        1 ccgcgtactt tcggcgcgag ctgcgcgtgt acctcgcacg aagggcggca agcctgcggt
       61 gcaacgggcc cggggcctgc ttgtttcttt gccggccaga cgccgagccg gacagagacg
      121 cctgcgggcg ccattcccga tccacagggg aaggatttat ctttacccgc cgggtgggaa
      181 aatgctatac ttgacccgtg cagtagggaa ctcatcgggg atgcggcgcg ctctgggaat
      241 agggcagccg atcgggacat gccctctccg gttgctggtg gaggcttgag ccttgcggat
      301 caccgagcga agtcactacg gactgcgggc catgatctgc ctggcccagc acagtccggg
      361 gcggccgatg tcagcgcggg agatctcggc gcgggagggg atcccgcccc gctacctcga
      421 gcaggtactg aaccgactca agaacggccg gctgatcatg gggcatcgcg gccccggtgg
      481 cggttacgtg ctggcccgcg atgcatcgga gatcagcgcc cgtgacatcc tggccgtggt
      541 ggaggacagc gacggcggtc gcggcggttt cctcgcggag atcgagtgcg ccgccgctcg
      601 ggggccggat ggcgcgaagg tgtgtggccc ggggtgcctg tcgaagcgcg tgtgggtgac
      661 cttggcggcg aaggtcgacg aagtgctgag ccagttccgg ctcgccgacc tggtcgcggg
      721 cgccggcggt gcggaggacg tgggcgaatg aagaccatct acttggacca cagcgccacg
      781 acaccggtcg acccggaagt cgtccaggcc atgttgccgt tcttcaccga gcggtacggg
      841 aatgcatcga gcatccatca cgccggacgc gatgcctacg acgcgatggg ggccgcgcgg
      901 gagtcggtgg cgtccctgat cggcgcatcg ccggacgaga tctacttcac cggtgggggc
      961 accgagtccg acaacctggc gatccagggc tacgcggggc acccggtcaa ccggaagcgt
     1021 ggcaaacaca tcatcacatc gaagatcgag caccacgccg tgctgcacac ctgtcagttc
     1081 ctcgagaggc agcgtggcca ccaggtgacg tactgcggcg tggatggcaa cggggtcatc
     1141 gacctggacg agctggcggc cagcatcgcc gatgacacca tcttgatcag cgtcatgctg
     1201 gcgaacaacg aagtggggac cattgaaccg ctgcgggaga tccgccggat cgtgggcgac
     1261 cggcccatcg cgatccacac cgacgcggtg caggcgctgg gcgagatgga catcgacgtg
     1321 aacgagctgg gcgtcgacat gatgaccctc tcggcccaca aggtctatgg cccgaagggg
     1381 gtcggggcgc tctatatccg gcgcgggacc cgggtggggc cgatcatgca cggcggcggc
     1441 cacgagcggc ggaagcggcc cgggaccgag aacgtgcccg gcattgtggg attcggcaag
     1501 gcgtgcgaac tgctggcgcg cgatggggcc gcggttcgcg agcgagtggc gcgtctgcgc
     1561 gatcgcttgg tcgaaggagt gatggcccgc gtggacgacg tggtcctcaa cggccatccg
     1621 acacggcgga tcccgcggaa cgccagtctg tgtttccgcg gctgcgaggg agaggcccta
     1681 ctgcttcatc tggatatggc gggcatctgc gcttcgagcg gctcggcgtg cacgtcccag
     1741 tcgctggagc cctcgcacgt gctggcggcg atgggagtgc ccatcgagat cgcgcatggg
     1801 gtgctccgtc tgaccctggg caagggcacc accgaggaag agattgatta cacggtcgat
     1861 acgttgacct cggtggtgga taagatacgc ggtatctcgg cggccctggg tgtgtgaggg
     1921 cccgggggcc gcgcccggtg gggcatcgag ggctcgaagc cagaaggtga gactagagtg
     1981 gcgatctaca cggagaaggt catggagcac atcgcctcac cgcggaacgt gggcgtgctc
     2041 gaggatgccg atggtgtggg aacggtcggc aacccggtgt gtggcgatat gatgaaaatc
     2101 tacatcaagg tcaatggcga ccggatcgac gacatccggt tccagacctt tggctgcggc
     2161 gcggcgatcg ccacgagctc gatcctgacc gagctcgcca agggcaagtc gctcgacgag
     2221 gccatggcca tcacgcgcga cacggtggcg cgcgagctgg ggggtctgcc cgcccagaag
     2281 atgcattgct cgaacctggc ggccgacggg ctacatgagg ccatccgcga ttaccaggcg
     2341 cggcgcgagg gccaggagtg aagcgccggc aggcgtgatg gcccccgagg gccggccgcc
     2401 atgcggacgg gtagccccgg ttgggttaca atggtctggg aaggtgccct cgcttctctg
     2461 gactctcccc gccgtgccac ggcgtgaagg agaccgacac cggggggtct accatgcggc
     2521 tggagctcga tgaacgcagg cggcgcgtcc tggccgccat cgtacgagac tatgtcagca
     2581 ccgcggagcc tgtggggtcc aagcgtctgg tggagcgcca cttgttccgg gtgagctcgg
     2641 cgacgattcg aaacgacatg tcggccctgg aggccgtggg gctcctggcc cagccgcaca
     2701 cctcggcggg ccggatcccg tcggaccgcg gctaccgctt cttcgtcgat gaactgatgg
     2761 agatcccggt gctatcaccc gctgcggagc gcattctccg gtcggcccgg gaacacacca
     2821 ccgacatcga cgacatgttg cgcgagatct gtaccatcct ctcgaaggtc actcagcaca
     2881 ccgcggtggc cctcgtgccc gggctcgaga aggcggagct ccggcatttc cagatcagca
     2941 aggtggacgg ggcgcggtgc ctgatcatcc tggtgacgga tgccgggcat gtggtccacg
     3001 agttcattga tgcaggcgag tcgtactcgg cccgcgagat cgaggtaatg agtggcattc
     3061 tgcggcggcg cctggtgggc atgaagatcg acgacattga ggcgctggac acgcgccacc
     3121 ttcgcgccga gccgcagctg aggggtctgc tgctcgagga gtcgttgcgc gtggtgcgtg
     3181 atcacctcga gcgcggcagt tactgggtgt tcgtggacgg tgccatgcac atcgtcaccc
     3241 agccggagtt caacgacgtg gagcgtctcc agcgcgtgct ccatgccctc gaacaagagc
     3301 agctcctggc gcagttgctc tccgagtgct tgaggacaca gg
//
LOCUS       DAOSPL010000360         3341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28608_length_3341_cov_2.844796, whole genome shotgun sequence.
ACCESSION   DAOSPL010000360 DAOSPL010000000
VERSION     DAOSPL010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28608_length_3341_cov_2.844796"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="PLQ54_07825"
     CDS             complement(<1..1258)
                     /locus_tag="PLQ54_07825"
                     /inference="COORDINATES: protein motif:HMM:NF024839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOS93205.1"
                     /translation="MHHARFATCRMGSTIVLALAVSTTGPWAQEAVEPIVSDATAVCL
                     VCHGPGGTGLAAGAPAIAQQWTGSAHATVGVGCAECHVVPKPGTGRDIGNPRYVVDTQ
                     WDRATGLKSVALVADDGKPRERPDLWAHQGAEVVVNVSPRTCATCHEAEAREFHGSRH
                     ASAAQFVGSIDNFLGRYAEGPAAAISGCQQCHGSEVRVSRAADGESPPEYAADTWPNT
                     GMGRVNADGSWGSCTACHSRHAFSPGVARRPDNCGRCHLGPDHPQAEVYRESKHGIAF
                     RATEASIGIDRPGLQWVLGKDYSQAPTCSSCHMGPVAARGQSPGLPMTHNAGARIAWT
                     LRPEVSVKPGAITEPDGRVVLSGPEQRRADMTTACQACHAPSWASNFFIQARADGEGF
                     SGATNVVATGFALTATYLGLALHQIGA"
     gene            complement(1292..2017)
                     /locus_tag="PLQ54_07830"
     CDS             complement(1292..2017)
                     /locus_tag="PLQ54_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOS93206.1"
                     /translation="MKSVRQIVHIDEDKCNGCGLCVPACHEGAIHIVDGKARLASDIL
                     CDGLGDCLGECPQGAITIEEREAEAYDADAVKARLAAHSSRATDVAQGGCPSARAWSR
                     ESAASAVAGSSEASASQLLNWPVQLHLVPPAAPYLRGARLLICADCVPFACADFHRDL
                     LAGHVVVTGCPKLDDGQLYIDKLAVLIAQNDLESIDIAFMEVPCCHGLVHIVRHAALQ
                     SGKEVPIQLTRVGIQGGLDHPEI"
     gene            complement(2014..2439)
                     /locus_tag="PLQ54_07835"
     CDS             complement(2014..2439)
                     /locus_tag="PLQ54_07835"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOS93207.1"
                     /translation="MSNALRISEAASIGLHTVLLLAQEGQQFLSARDMARTMGVSEAH
                     LSKVLQRLVRAGIAESARGPHGGFRLAAGAEALSLLAVYEAIDGPLRPLPCLLGRPMC
                     DGKSCILGGLLASVNEQIRDQLASVRLSQFLARASGEAQ"
     gene            complement(2626..>3341)
                     /locus_tag="PLQ54_07840"
     CDS             complement(2626..>3341)
                     /locus_tag="PLQ54_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOS93208.1"
                     /translation="FYHDLMHVHNVVPTAAQLASISSQGGWGSGGLTLFSGGKAAMIS
                     IGRWYIIQVPNYPQIAGALGAVRLPRVGNRESSGMTDARAAGINIKSHHIQEALKFLQ
                     YLASPEYSALIVKDGDSLPPNPLLAQSGKALVNPAVPDPAFHQPFVDAEKSARPVDTS
                     PFVDALQVQRWLVEHIDKVENKLMTPKEAMKELTHAINQQIRINLERQPHLQRKYERV
                     TGRPYEPGWWRQYQGTRVD"
BASE COUNT          554 a         1134 c         1110 g          543 t
ORIGIN      
        1 acgcgccgat ctgatgcagc gcgaggccta ggtaggtggc ggtcagtgcg aacccggtcg
       61 ccacgacgtt ggtcgccccg gagaatcctt cgccgtcggc ccgggcctgg atgaagaagt
      121 tgctggccca gctcggtgca tggcacgcct ggcacgcggt cgtcatgtcg gcacgtcgct
      181 gttcgggccc actcagcaca acgcggccat cgggttcagt gatggcaccg ggcttcaccg
      241 acacctcagg gcggagggtc cacgcgatgc gcgccccggc gttgtgggtc atggggagcc
      301 cgggcgactg gccccgcgcc gccacaggcc ccatgtggca cgaggagcac gtaggggctt
      361 gggagtagtc cttgcccaac acccactgca gcccgggccg gtcgattccg atcgatgcct
      421 cggtcgcgcg gaaggcgatg ccgtgcttgg actcacgata gacttcggcc tgagggtggt
      481 ccggtcccag atgacaccgg ccgcagttat caggcctccg cgccacaccc ggtgagaacg
      541 catggcgtga gtggcaggcg gtgcacgacc cccacgaacc atcggcgttc acgcgcccca
      601 tgcccgtgtt gggccacgtg tcggcagcat actccggcgg ggactcacca tcggcggcgc
      661 gggagacacg cacctcgctg ccgtggcact gctggcaccc gctgatcgcc gcggccgggc
      721 cctcggcata gcggccgaga aagttatcaa tactgccgac gaactgcgcg gccgaagcat
      781 gccgggagcc gtggaactcg cgcgcttcgg cctcatgaca cgtggcgcac gtccgcggcg
      841 atacgttgac cacaacctcg gcgccctgat gcgcccacaa gtccggtcgc tcccggggct
      901 tgccgtcgtc cgccaccagt gccaccgatt tcaggcccgt ggcacggtcc cactgggtat
      961 cgaccacgta gcgtgggttc ccgatgtccc tgccggtgcc aggcttcggc acgacgtggc
     1021 actcggcgca acccactccc acggtggcgt gggcgctccc cgtccactgc tgcgcgatgg
     1081 ccggcgcccc cgcggccagc ccagtgccgc ccggtccgtg gcacacaagg cagacggccg
     1141 tcgcgtcgct gacgatcggt tcgacggcct cctgggccca cgggcccgtg gtcgaaacgg
     1201 ccagcgcgag cacgatcgtt gatcccatgc gacatgtggc gaacctagcg tggtgcatca
     1261 gagcctccgt tcgcagtccc ggagcccgac cctagatctc cgggtgatcg agtccgccct
     1321 ggatgccgac tcgcgtcaac tgaataggca cttccttgcc cgactgcagg gcggcgtggc
     1381 gaacgatgtg aaccagccca tggcagcagg gcacctccat gaacgcgata tcgatggact
     1441 ccagatcgtt ctgcgcgatg agcacggcga gtttgtcgat gtagagctgc ccgtcgtcca
     1501 gcttggggca tccggtgacc acaacatgac cggcgagaag atcccggtgg aagtcggcgc
     1561 aggcgaaagg aacacagtcg gcacagatca ggagcctggc accccgcagg tacggtgccg
     1621 cggggggaac aaggtggagc tgaacaggcc agttcaacaa ctgtgaggcc gaagcctccg
     1681 aactgccggc cacggccgac gcagccgatt cgcgagacca tgcccgcgcc gaagggcaac
     1741 ctccctgagc cacatcggtg gcgcgagatg agtgggcggc cagacgggcc ttcaccgcat
     1801 ccgcgtcata ggcttcggcc tcgcgctcct caatggtgat cgcgccctgg gggcactcac
     1861 ccaggcagtc gccgaggccg tcacacagga tgtcgctcgc gagccgcgcc ttaccatcca
     1921 cgatgtggat ggcgccttcg tggcacgccg gcacgcacag gccacagcca ttgcacttgt
     1981 cttcgtcgat gtggacgatc tggcgaacgc tcttcattgc gcctcccccg aagcccgggc
     2041 taggaactgc gacagtcgga ctgacgccaa ctggtcgcgg atctgctcgt tgacgctggc
     2101 cagcaagcca ccgaggatgc agctcttgcc gtcgcacatg ggccgcccga gtaggcacgg
     2161 cagaggccgg agcggcccat cgatggcctc atagacggcc agtagcgaca gggcctcggc
     2221 accggcggcc aggcggaaac cgccgtgggg gccgcgtgcc gactcggcga tgcccgctcg
     2281 cacgagccgc tggagcacct tcgacaggtg cgcctcggag acgcccatcg tccgggccat
     2341 gtccctggcc gagaggaact gctggccctc ctgggccagc agtaggacgg tatgcagccc
     2401 gatggacgcc gcttccgaga tcctcaatgc gttggacatg ccgcatactc caatgccgaa
     2461 ttgcagtacc gtaatacctg aatgcactcg ccagacgaag agttcccgga tcggcacgaa
     2521 tctgctggcc ccgggttcag ccgcgccctg ggccggaccc aagtccgtgg ccgatcatag
     2581 agcgatcgcg tgaagccggg gtgatgggcg atggacgggg tggtgctagt ccacgcgagt
     2641 gccctggtac tgtcgccacc aacccggttc gtagggacgg cccgtgaccc gctcgtactt
     2701 gcgctgcaag tggggctgcc gttcgaggtt gatccgtatc tgctggttga tggcgtgggt
     2761 cagttccttc atggcctctt tgggcgtcat gagcttgttc tcgaccttgt cgatgtgctc
     2821 aacgagccat cgctgcacct ggagcgcatc gacgaacggg ctcgtatcca ccggccgggc
     2881 gctcttctcc gcgtccacga agggctgatg gaaggccggg tcaggcacgg cgggattcac
     2941 cagcgccttg cccgactggg ccaggagcgg gttgggtggc agcgagtccc cgtccttcac
     3001 gatcagcgcg ctgtactcgg gactggccag gtactgaagg aacttgagcg cttcctggat
     3061 gtggtggctc ttgatgttga tgcccgccgc ccgggcgtcc gtcatccccg atgactcccg
     3121 gttgcccacc cgcggtagcc gcaccgctcc cagggcgccc gcaatctgcg ggtagttcgg
     3181 cacctggatg atgtaccacc ggccgatgct gatcatggcg gctttgccgc ccgagaacag
     3241 cgtcagcccg ccactacccc agccgccctg ggacgagatg gaggccaact gcgccgcggt
     3301 cggcaccacg ttgtgcacgt gcatcaggtc gtggtagaac c
//
LOCUS       DAOSPL010000361         3335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28661_length_3335_cov_3.308232, whole genome shotgun sequence.
ACCESSION   DAOSPL010000361 DAOSPL010000000
VERSION     DAOSPL010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28661_length_3335_cov_3.308232"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..>1612)
                     /locus_tag="PLQ54_07845"
     CDS             complement(119..>1612)
                     /locus_tag="PLQ54_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93209.1"
                     /translation="DGVCTIDSDPGGWLGSPVSDYMKILRGCRALLDKHNIHGRDAKI
                     INWLWTGWGHRLEIPGNLSVIADTVRAMKAEMPEPWRLIAGTEAYLPICQAEGVLDHT
                     IYVPYGAIEGEPSYPWTNIGLGGIDGLFAGAAKYPGLLGVMGNTQCPLLQFPRVRAFL
                     QLAWDPSAAQRSEEDRLLDVSRELYGDHAELIARCFAAVCTGGPEQLEAVANRLDRLV
                     RAGELGPPGVLGQSLFPDPGFVARSLLMQLRVRLAAERLYDAVADGAKAAECLQRVEA
                     LLDAYLAWDDAHGWHELWGPGPWQLGNLPADPRFVAALSYLRWVIGDDAALNAFFDQV
                     AEGLSAGRDAASVTANAIEPMRTRVLSAVVLKPNAAREATVSASALPDAAKYPPEHAI
                     DGDISTLYWPGALVQDNEEWFEIAWPEPRTFRRVDAYFLRHESMWNRTIHLQRPSGAG
                     AWEDIATCTPTDTTAHALACFELSAPVTAGRIRLVNLLDLFEVEVLE"
     assembly_gap    1613..1622
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1623..2470)
                     /locus_tag="PLQ54_07850"
     CDS             complement(<1623..2470)
                     /locus_tag="PLQ54_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93210.1"
                     /translation="MQEMIALPYLVLTAMAAQSAPDVSLEGCTIVAPAFQPTHLARAV
                     KDLRNRLLQGRLVPVRTHLPEGTGPAIIVGADAARRVGIELPPGEELGPEGYVLRVTK
                     GAGRTLLVAAGATPRGTQQAVIALTREIRTEGRNQYIPANLDRTTKPAYAVRGMHLNG
                     WPMHRPYSFRSWNENDWCRYIDMLAHMGANLVYLWPFMDIMPVPLSAEDEEYLHEFRR
                     VVDYAQKQHGMEVWIMQSANRVARDTCGVADPRHRPYWRPSQADRNPADPAEFAAIME
                     SREALYR"
     gene            complement(2580..3176)
                     /locus_tag="PLQ54_07855"
     CDS             complement(2580..3176)
                     /locus_tag="PLQ54_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93211.1"
                     /translation="MSNPITVDLFVEDAGHENLLRPLVIRVARRQRREVRVRVRSGRG
                     GHGKALQEFVAFQKILERGVGDPQPDLIVVAIDANCLAFTEARKSVLRATLPKFQSIC
                     VPACPDPHVERWYLADAQAFGAVVGGTPTVPRRKCERDVYKRALAQAVVDAGHPPELG
                     GIEFGPELAEAIDLHRAGRSDSSLKHFIDDLAGRLKRL"
BASE COUNT          509 a         1136 c         1140 g          540 t
ORIGIN      
        1 cgaccactcg gcgccccagg ggcagctcac gtgggtcgaa tgggtcgacg agggccatct
       61 gtggccggac ttcgcggcca agctgagggc atggtcgccg cggtagactt gggtcccctc
      121 actccagcac ctcgacctcg aacaggtcca gcaggttcac caaccggatc cggcccgccg
      181 tcacgggcgc cgacagctcg aagcacgcga gcgcgtgcgc ggtcgtgtct gtaggcgtgc
      241 aggtcgcgat gtcctcccaa gccccggcgc cgctgggccg ttgcaggtgg atcgttcggt
      301 tccacatcga ctcgtgtcgg aggaagtagg cgtcgacgcg gcggaaggtc ctgggttcgg
      361 gccacgcgat ctcgaaccac tcctcgttgt cctgcaccag cgcgcccggc cagtagaggg
      421 tggagatgtc gccgtcgatg gcgtgctcgg gtgggtactt ggcggcgtcg ggcagtgcgg
      481 acgcggagac cgtggcctcc cgcgccgcgt tgggcttgag cacaacagcc gagaggacgc
      541 gggtgcgcat cggctcgatg gcgttggcgg taacgctcgc ggcgtcgcgt ccggcagaga
      601 gcccctcagc gacctggtcg aagaaggcgt tcagggcggc gtcgtcgcca atcacccacc
      661 gcaagtaaga gagtgccgcg acgaagcgcg gatcggccgg caggttgcca agctgccacg
      721 gccccgggcc ccagagctcg tgccagccgt gggcgtcgtc ccaggcgagg tacgcgtcga
      781 ggagcgcctc gacgcgctgc aagcactcgg ccgctttggc cccgtccgcc accgcgtcgt
      841 agagccgctc cgcggccagg cgaacccgca actgcatgag cagcgatcgt gcgacgaagc
      901 ccgggtcggg gaacagcgac tggcccagca cgcccggcgg gccgagctcg ccggcacgga
      961 ccagtcggtc cagccggttt gccaccgcct ccagctgctc agggccgcct gtgcacaccg
     1021 cggcaaagca ccgggcgatg agctccgcgt ggtcgccata gagctcgcga gacacgtcga
     1081 gcagccgatc ctcctccgac cgttgggcag ccgaagggtc ccacgccaac tggaggaatg
     1141 cgcgtacgcg cgggaactga agcagggggc actgcgtgtt gcccatcacg cccagcagac
     1201 ccgggtactt ggctgcgccc gcgaagaggc cgtcgatgcc cccaagaccg atgttcgtcc
     1261 acgggtatga gggctcgccc tcgatcgctc cgtagggtac gtagatggtg tggtcgagca
     1321 cgccctcggc ctggcagatc ggcaggtagg cctccgtgcc ggcgatgagc cgccatggct
     1381 cgggcatctc ggccttcatg gcgcgcaccg tgtcggcgat gacggacagg ttccccggga
     1441 tctcgagccg gtgcccccag ccggtccaca gccagttgat gatcttcgcg tcgcggccgt
     1501 ggatgttgtg cttgtccagc agtgctcggc agcctctgag gatcttcatg tagtcggaga
     1561 ccggactgcc gagccagccg ccgggatccg agtcgatggt gcatacgcca tcnnnnnnnn
     1621 nncggtagag cgcctcacgc gactccatga tggcggcgaa ctcggcgggg tctgccgggt
     1681 tgcgatccgc ctgcgagggt cgccagtagg gccgatgccg cggatcggcc accccgcacg
     1741 tgtctctggc cacgcggttg gccgactgca tgatccagac ttccatgccg tgctgcttct
     1801 gtgcgtaatc gaccacgcgg cggaactcgt gcaggtactc ctcgtcctcg gcggatagcg
     1861 gcacgggcat gatgtccatg aagggccaga ggtacaccag gttggccccc atgtgtgcga
     1921 gcatatcaat gtagcggcac cagtcattct cgttccacga gcggaagctg tagggccggt
     1981 gcatcggcca cccgttgagg tgcatgcctc gtaccgcgta ggccggcttg gtggtgcggt
     2041 cgaggttggc cgggatgtac tggttccggc cctccgtgcg gatctcgcgt gtgagcgcga
     2101 tcaccgcctg ctgcgtgccc ctgggagtcg ctccggcggc gacaaggagc gtgcggcctg
     2161 cccccttggt gacccggagc acgtagccct cggggcccag ttcctcgccc ggaggcagtt
     2221 cgatgcccac gcgccgcgct gcatccgctc cgacgatgat ggcgggcccg gtgccttcgg
     2281 gcaggtgcgt ccgcaccggg accaggcgcc cctgcagcag tcgatttcgt aggtccttca
     2341 cggcccgcgc caggtgcgtt ggctggaacg cgggtgccac gatggtgcac ccttcgagcg
     2401 atacgtcggg cgccgactgc gccgccatcg cggtgagtac caagtatgga agggcgatca
     2461 tctcctgcat cgcaggtcct ctccccgacc acgaacccgg cggcagcgcc gcccgacgtg
     2521 cgctcgacat gctcgcgatc cccgcggttg ccgccgcatc attcgtggga gtgtggccgt
     2581 cacagccgct tgagcctacc ggcaaggtca tcgataaagt gcttcaggct gctgtctgag
     2641 cgtccagccc ggtggaggtc gatcgcctcg gcgagctcgg ggccgaactc gatgccccca
     2701 agctccgggg ggtgcccggc gtcaacgacg gcctgtgcca gcgcccgctt gtagacgtcg
     2761 cgctcgcact ttcgcctcgg aaccgtcggc gtccccccca ccaccgcgcc gaaggcctgg
     2821 gcatccgcca ggtaccaccg ctcgacgtga ggatcggggc aggccggtac gcagatgctc
     2881 tggaacttgg gcaaggtggc gcgcaatact gatttccggg cctcggtgaa cgcgaggcag
     2941 ttggcatcga ttgccaccac gatcaggtca ggctgcgggt caccgacgcc ccgctctaga
     3001 atcttctgga acgccacgaa ctcctggaga gctttgccgt gtcctcccct tccggaccga
     3061 acgcgcaccc ggacctccct gcgctgcctg cgggcaactc gtatgaccag aggccggagc
     3121 aggttctcgt gccccgcgtc ctccacgaac aggtcgacgg tgatggggtt actcatcgag
     3181 ccacccccgg agcagcatct cctggaacag gccatcctcg gccggcgagg tgagcccgtc
     3241 ggcgagcccg gtgtcgacga aaagcgttcc cggttcctcg gcgaaggggg tcaccttggt
     3301 cccttcccta tcgcggcgga ccgcgtagag cccaa
//
LOCUS       DAOSPL010000362         3335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28664_length_3335_cov_2.939634, whole genome shotgun sequence.
ACCESSION   DAOSPL010000362 DAOSPL010000000
VERSION     DAOSPL010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28664_length_3335_cov_2.939634"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLQ54_07860"
     CDS             complement(<1..222)
                     /locus_tag="PLQ54_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS93212.1"
                     /translation="MRRGFTLIELLVVIAIIAILAAILFPVFAKAREKARQASCLSNV
                     KQMSLSLDRYTHDADECLPPMPCGCNYVPG"
     gene            385..1221
                     /locus_tag="PLQ54_07865"
     CDS             385..1221
                     /locus_tag="PLQ54_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOS93213.1"
                     /translation="MAPLTLALSAALTLQADARPQPPEALPAPTRETFNCTADGSCWP
                     YLIQTPEAPQAVVLYLHGHYGDEYQGMAEGSYNDCFGKLRRECIRRQWAYVTTWYGGN
                     TWMGPLAEQGVAELITALRVRWPGVPVYLTGGSMGGSSALVFAHVRPDLVDGVVALCP
                     AGDIETYYRFASASAEPVLANIASAIRIHYTIDGHELMAELDRRSAARHAENLTMPVY
                     IIHGAKDTLIPIDGTRALVERLRALKRPIEYIEQPEGEHDTPVTVVDWPAALDFISAA
                     DH"
     gene            complement(1312..2397)
                     /locus_tag="PLQ54_07870"
     CDS             complement(1312..2397)
                     /locus_tag="PLQ54_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93214.1"
                     /translation="MCARTTWLARLTAALVLALLVAGAPPGWVQSGTLSPAELAKVIL
                     DAAKLPGLIEGYITEVQVLPPGPPLPPPAKPDDPVQVYEFPQDEAFQIAGRIVLMTMT
                     SFRCAPGAYLVDDTRFQVTSEGPGATPKRELYWFDGAGGERFFMPGYVAQHVAPGDVS
                     VTPEPGRGLDRLESALQADLGLEQGQIRRLLKRWDGAVVTVTPGITRLGLECVRVSCR
                     KTDPIPVEWVEWFAPSRSYHCVERIQRSHPLNGHTKFHQMRVERFAEVIDGYWVPAMT
                     RYTGTDLAPDGVSVVVINSVTTCTGIRTRDAATLAMEPLLPNGTAVYEGTNRSVIGDD
                     TRDLELQLRSGQLPPVLPVPDLEAEGL"
     gene            2699..>3335
                     /locus_tag="PLQ54_07875"
     CDS             2699..>3335
                     /locus_tag="PLQ54_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS93215.1"
                     /translation="MLYRPFGKTGLEVSALGFGAMRLPEDTDEAVAVLTRAFDQGVNF
                     LDSARGYPNSEAKCGKALKGRREQIVVSTKVPSWIEPVVEGWRERFEESLTALDTSYI
                     DVLQILHDLKWEAFEKHVAPKGKVLDLVTQARDEGLVRYFSFSSHDTPENVMKLIDTG
                     VFDAMIVQYNLLDLQYADAIDHACARGMGVVAMGPVGGGRLAGLSPELARMA"
BASE COUNT          598 a         1074 c         1102 g          561 t
ORIGIN      
        1 gccggggacg tagttgcagc cgcagggcat gggtggcagg cattcgtcgg catcgtgcgt
       61 gtacctgtcc agtgacagtg acatctgctt gacgttggag aggcaactgg cttggcgggc
      121 cttctctcgc gctttggcga agacagggaa caagatggcc gccaggatgg cgatgatggc
      181 gataacgacc agcaactcga tcagcgtgaa gcctcgtcgc atcgtgtgcc ctccctacgt
      241 gtcctgccgt cgatgtcgga ctgccatatc caagttcgcc actcaggatc gtcagccctt
      301 cagctccggg gcggtgttcg aagggtcgag agggaaccgt cgcgaaccat acccaagctc
      361 ccgccagccc ccaggaggca atcgatggca ccactgacac ttgcgctctc cgccgccctg
      421 acgctccagg cagacgcccg accgcaaccg cctgaggcgc tgcccgcccc caccagagag
      481 accttcaact gcacggccga cggatcgtgc tggccgtacc tgatccagac tccggaggcg
      541 ccccaagctg tcgtcctcta cctccacggg cactacggcg acgagtacca aggcatggcc
      601 gagggcagct acaacgactg cttcggcaag ctccggcgcg agtgcatccg ccgtcagtgg
      661 gcgtatgtga ccacctggta tggcggcaac acatggatgg gcccgctggc cgagcaaggc
      721 gtggccgagc tcatcacggc gttgagggtg cgctggcccg gcgtgccggt gtacctcacc
      781 ggtggctcca tgggcggatc ctcagcactg gtcttcgccc atgtccgtcc tgacctggtt
      841 gacggtgtcg tcgccctctg cccggccggc gatatcgaga cctactaccg cttcgcatcc
      901 gcatcagccg agccggtgct ggcgaacatc gcctcggcca ttcgtatcca ctacaccatt
      961 gacggccacg agctcatggc cgagctcgac cgccgctcgg cggcccgcca tgccgagaac
     1021 ctgacgatgc ccgtctacat cattcatggg gccaaggaca cgctcatccc catcgatggt
     1081 acacgggcac tggtcgagag gctgcgcgct ctgaagcggc cgatcgagta catcgagcag
     1141 ccagaaggcg agcatgacac cccggtcacc gtagttgact ggccggccgc actggacttc
     1201 atatcggccg ccgatcacta gctacctgtg cttcgatcgg tcacacagcg gcctggtcga
     1261 acggcgcgga gctgcagctg caggtcgcgc gtgtaagcgc cgatggcgct ctcataggcc
     1321 ctcggcctcc aggtcgggta ccggcagtac cggcggaagc tgaccggagc ggagctgcag
     1381 ctccaggtcg cgcgtgtcat cgccaatgac ggaacgattg gtcccctcgt agactgcggt
     1441 gccgttcggc aacagcggct ccatcgcaag agtggcggcg tcacgcgtac ggatgcccgt
     1501 gcacgtcgtg accgagttga tgacaactac cgacacgcca tcgggtgcca agtcggtgcc
     1561 ggtataccgg gtcatggcgg gcacccagta gccgtcgatc acctccgcga agcgctcgac
     1621 ccgcatttgg tggaacttcg tgtgcccgtt gagcgggtga ctccgctgga tgcgctcgac
     1681 gcagtggtag gaacggctcg gcgcgaacca ctccacccac tccacgggga ttggatcggt
     1741 cttccggcag gacacacgga cacactcgag cccgaggcgg gtgatgcccg gtgtgaccgt
     1801 aacgacggcc ccgtcccagc gcttgagcag gcggcggatc tgtccctgct cgaggcccag
     1861 gtcggcctgc aacgcagact ccaggcggtc gagtcctctc ccaggctcgg gcgtgacgct
     1921 cacgtctccc ggtgccacat gctgcgccac atagcccggc atgaagaagc gttcgccacc
     1981 ggcgccgtcg aaccagtaga gctcgcgctt tggggtcgca ccggggccct ctgaggtcac
     2041 ctggaagcgg gtgtcatcga ctaggtaggc cccgggggcg cagcggaacg aggtcatagt
     2101 catcaggacg atccggccgg cgatctggaa tgcctcatcc tgtgggaact cgtagacctg
     2161 cacggggtca tccggcttgg ccggtggcgg cagcggcggg ccgggtggca ggacctggac
     2221 ctcagtgatg tagccctcga tgagcccggg cagcttggcg gcgtcgagga tgacctttgc
     2281 gagctcggcg gggctgaggg tcccggattg tacccagcct ggcggcgctc cggcgacgag
     2341 cagcgcaagg acaagagcag cggtgagtcg cgccaaccag gtggtgcgcg cacacatggc
     2401 tacagacctc ctctgcggcg ttgcggcatg gtggccgacg acgaacccat tgacgatcgg
     2461 cacggcattc cggatgccct gcgaggcgga ggggttgggt gaagacgcta taactgcgga
     2521 atcccgacat cagcaatccg gagtcaggag tcggggagga ccggcaacgc catggcgctg
     2581 cttgggccca acggctatcg ccatcgggtg catgcgtgta gaatgggacc ccgtaagcgc
     2641 gatggtgcag gctgcagtaa ctccatggat tccccactcc ggaaggtggc acgcgcgcat
     2701 gctgtaccgt ccatttggca agaccggcct tgaggtgtcg gccctcggtt tcggagcgat
     2761 gcgtctgccc gaggataccg acgaggccgt ggccgtcctc acccgggctt tcgaccaggg
     2821 cgtgaacttc ctggactccg cccggggcta ccccaacagc gaggccaagt gcggcaaggc
     2881 actgaagggc cgacgcgagc agatcgtcgt ctcgaccaaa gtcccgagct ggatcgagcc
     2941 cgtggttgag ggctggcggg aacggttcga ggaatcgcta acggctttgg acaccagcta
     3001 cattgacgtg ctgcagatcc tccacgacct gaaatgggaa gcattcgaga agcacgtggc
     3061 accaaagggc aaggtcttgg acctggtcac ccaggcgcga gacgaagggt tagtacggta
     3121 cttctcattc tcttcgcacg acacgcccga gaatgtcatg aagctgatcg acaccggtgt
     3181 cttcgacgcg atgatcgtgc agtacaacct gctggacctg cagtatgccg acgccatcga
     3241 ccatgcctgt gcgaggggca tgggcgttgt cgccatgggc cccgtgggtg gcggccggct
     3301 ggccgggctg tcgcccgagc tcgccagaat ggcgc
//
LOCUS       DAOSPL010000363         3333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28681_length_3333_cov_4.120195, whole genome shotgun sequence.
ACCESSION   DAOSPL010000363 DAOSPL010000000
VERSION     DAOSPL010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28681_length_3333_cov_4.120195"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..3332)
                     /gene="carB"
                     /locus_tag="PLQ54_07880"
     CDS             complement(126..3332)
                     /gene="carB"
                     /locus_tag="PLQ54_07880"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOS93216.1"
                     /translation="MPKRDDVHKVLIIGSGPIIIGQACEFDYSGTQACKALRALGYQI
                     VLVNSNPATIMTDPGMADATYVEPLTPESIEQIIARERPDALLPNLGGQTGLNLTAEL
                     HRRGILDRYGVQVIGVQVDAIERGEDRIAFKDTMSSLGIPMPESSPCFSVEEAEAIAA
                     RLGYPVVLRPAYTLGGTGGGIAWNVEDLRTIVARGLAASLVGQVLVEESVLGWEELEL
                     EVVRDADNNKITVCFIENVDPMGVHTGDSYCTAPMLTVAPELQERLQEISYRIVEAIG
                     VIGGTNIQFAHDPATGRVVVIEINPRTSRSSALASKATGFPIALVSAKLAGGLTLSDI
                     PYWRDGTLDKYTPSGDYVVVKFARWAFEKFDDAADQLGTQMRAVGEVMSIGKTYKEAF
                     QKSIRSLEIGRYGLGFAKNYHDLPLADLRRLLAEPTSERQFVMYEALRKGMPVDELHS
                     LTHIGRWFIEQMAELVALEERILKHAGSLPPDDLLLQAKRDGFSDRYLGQILGVGEEA
                     VRERRRALGLAQAWEAVPVSGANASYYFSTYNASDSVPVSDRKKIMILGGGPNRIGQG
                     IEFDYTCVHAAFALRDLGYESIMVNCNPETVSTDYDTSDKLYFEPLTVEDVLSIYDKE
                     KPEGVIIQFGGQTPLNLAAELAAAGVRILGTSPDSIDLAEDRKRFAEVMRQLHIPQPE
                     SGTASSLEEGLEVARRIGYPLMVRPSYVLGGRGMQVVYDEEMFVRYVTAAVGITPEHP
                     ILIDKFLENAVEAEADAIADGEDAFVPSVMEHIELAGIHSGDSACVIPPLTIAPEHLA
                     TIEDYTRRIARALHVVGLMNMQYAICDGTVYVLEANPRASRTVPLVSKVTGVSMARMA
                     TQVMLGAKLADLDTRRRHYSHFGVKESVFPFNMFPEVDPVLGPEMRSTGEVLGMADCF
                     GLAFYNAQLGAGLGLPVSGTVLVTVADRDKEAVLPSVRKLADMGFRVMATAGTAAYLE
                     SHGVAAEVARKLYEGRPHILDALHNREVAMVINTPAGKASAHDDSYIRKAAIRYKIPY
                     VTTPAAAAAAAEGIRAARERASAVKSLQQYHKDVTTSG"
BASE COUNT          548 a         1064 c         1108 g          613 t
ORIGIN      
        1 ccctggccgc ggccagggcc gcgcagacac cactcacgcc cgcgccggcg accagaacgt
       61 cggccgagtc ggccacacgg acccgcctcg ccggcacgac cagggcctcc ataaccactc
      121 ctccgctacc ccgaggtcgt cacatccttg tggtactgct gcagtgattt caccgctgag
      181 gccctttcgc gggccgcgcg gataccttcg gccgccgccg cggctgctgc gggggtggtg
      241 acgtagggga tcttgtaacg gatggcggcc ttgcggatgt agctgtcgtc gtgggcgctc
      301 gccttgcccg ccggggtgtt gatcaccatc gcaacctcgc ggttgtggag cgcatcgagg
      361 atgtgcggcc ggccctcgta cagcttcctg gccacctcgg cggccacacc atggctttcg
      421 agataggcgg ccgtacctgc ggtggccatc acgcggaagc ccatgtcggc cagctttcgc
      481 accgagggga gtacggcctc tttgtcgcgg tccgccacgg tgaccagtac ggtcccgctg
      541 accggaaggc ccagtcccgc tccgagctgt gcattgtaga acgccagtcc gaagcagtcg
      601 gccatcccga gcacctcgcc cgtcgatcgc atctcgggcc ccaggaccgg atcgacttcg
      661 gggaacatgt tgaaggggaa caccgactct ttcacaccga agtggctgta atgccggcgc
      721 cgcgtatcca ggtcggccag cttcgccccc agcatgacct gggtagccat gcgtgccatc
      781 gatacgccgg tcactttcga caccagcggc accgttcggg atgcacgcgg gttggcttcg
      841 aggacgtaca cggtgccgtc acagatggcg tactgcatgt tcataagccc caccacatgc
      901 agggcccggg cgatccgtcg tgtgtagtct tcgatggttg ccaggtgctc cggagcgatg
      961 gtgagcggcg ggatgacgca cgcgctgtca cccgaatgga tacccgccag ctcgatgtgt
     1021 tccatgaccg agggcacaaa ggcatcctcg ccgtcggcga tggcgtcggc ctcggcttcg
     1081 acggcgtttt cgaggaactt gtcgatcaga atcgggtgct cgggcgtgat ccccaccgcg
     1141 gcggtgacgt agcggacgaa catctcctca tcgtagacca cctgcatacc tcggccgccc
     1201 agcacgtagg acggacggac catcaggggg tagccgatgc ggcgagccac ctcgaggcct
     1261 tcttcgagcg atgaggcggt gccgctctca ggttgtggaa tgtgcagctg ccgcatgacc
     1321 tcggcgaagc gcttgcggtc ctcggcgagg tcaatgctgt ctggagacgt gcccagaatc
     1381 cgcaccccgg cggcggccaa ttccgcggcc agattcagcg gtgtctggcc cccgaactgg
     1441 atgatcaccc cctctggttt ctctttgtcg tagatggaca gcacatcctc gacggtgagg
     1501 ggctcgaagt agagcttgtc tgaggtgtcg tagtccgttg agaccgtctc cgggttgcag
     1561 ttgaccataa tcgactcgta acccaggtcc ctgagggcaa aggcggcatg gacacacgtg
     1621 tagtcgaact cgatgccctg cccgatgcga ttgggcccgc cgccgaggat catgatcttc
     1681 ttccgatcgg atacgggcac ggagtcggaa gcgttgtagg tcgagaagta ataggaggcg
     1741 tttgccccgc tcacgggcac cgcctcccag gcctgagcca gacccagggc ccgccgccgt
     1801 tcgcgaacgg cctcctcacc gacgccgaga atctggccca ggtagcggtc ggagaacccg
     1861 tcgcgcttcg cctgcagcag caggtcgtca gggggcaggg atccggcgtg cttcaggatc
     1921 cgctcctcga gggccaccag ctcggccatc tgctcgatga accaccgccc gatatgtgtc
     1981 aagctgtgga gctcgtcaac cggcatacct ttgcggagcg cctcgtacat cacgaactgg
     2041 cgttctgacg tgggctcggc caggaggcgt cgaaggtcgg cgagtggcag gtcgtggtag
     2101 ttcttggcga agcccagccc gtaacgtccg atttcgagcg accggatcga cttttggaat
     2161 gcctccttgt acgtcttgcc gatgctcatg acctcgccga cggcgcgcat ctgggtgccc
     2221 agctggtcgg cggcgtcgtc gaacttctcg aaggcccatc gggcgaactt cacgaccacg
     2281 tagtcgcccg agggggtgta cttgtcgagg gtgccgtcgc gccagtaggg gatgtccgag
     2341 agggtcagcc cgccggccag tttggccgac acgagggcga tggggaagcc cgtagccttc
     2401 gacgccagag ccgacgacct ggaggtgcgc gggttgatct cgatcacgac aactcgcccg
     2461 gtcgcggggt cgtgcgcgaa ctggatgttg gtgcccccga tcacaccgat ggcctcgaca
     2521 atgcggtagg agatctcctg aaggcgctcc tggagttccg gggcaacggt cagcatgggc
     2581 gcggtgcagt atgagtcgcc cgtgtgcacg cccatggggt cgacgttctc gatgaagcac
     2641 accgtgatct tgttgttgtc ggcatcgcgg acgacctcga gttcgagctc ctcccagccg
     2701 aggaccgact cctcgaccag cacctggccc acgaggctag ccgcgaggcc tcgggccacg
     2761 atggtgcgca agtcctccac gttccaggcg attccgccgc cggtgccgcc cagtgtgtag
     2821 gcgggccgca agacgacggg atagcccagg cgagccgcga tggcttctgc ctcctcgacg
     2881 ctgaagcagg gtgagctctc cggcatgggg atgccgaggc tgctcatggt gtccttgaag
     2941 gcgatccggt cctcgccgcg ttcgatggcg tccacctgga caccgataac ctgaacgccg
     3001 tagcggtcga ggatgccgcg tcgatggagc tcggccgtga ggttcaggcc ggtctggccg
     3061 cccaggttgg ggagtagggc gtcgggcctc tcgcgggcga tgatctgctc gatgctctcc
     3121 ggggtcaggg gctctacgta agtggcatcg gccatgccgg ggtcggtcat gatggtggcc
     3181 gggttcgagt tcaccagcac gatctggtat cccagcgctc ggagagcctt gcacgcctga
     3241 gtaccggagt agtcgaactc gcaggcctgc ccaatgatga tggggccgct gccaatgatg
     3301 agtactttat gaacgtcgtc tcgcttgggc atg
//
LOCUS       DAOSPL010000364         3323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28788_length_3323_cov_2.713892, whole genome shotgun sequence.
ACCESSION   DAOSPL010000364 DAOSPL010000000
VERSION     DAOSPL010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28788_length_3323_cov_2.713892"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1593)
                     /locus_tag="PLQ54_07885"
     CDS             complement(130..1593)
                     /locus_tag="PLQ54_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOS93217.1"
                     /translation="MRNTSQRPDEHRITRRDFVRSAALTAGAVGLAGTMGHAQEADPV
                     AEQGPIRSRFLWTWDHCTTWALNRAGGHDMGASNDYGRTTESFIADYSSALRWCGKHG
                     IEGIVVWGLLRDRHGGLESVKRLCEVAKESGTKLLAGVGLNAYGGVYYEGNSPWSLEQ
                     HLKANPGLYGIDAKGERMIFDFGFAGQKLCHHACPSRPENQEYAAESLRWLMETTDID
                     GVQMEAGDTGVCQCPTCKARRRHPVSGFSWEDMALIYAKATEAIRSVRPEATVIVETY
                     SHPQPHESPEEAPGFGGGAPEWAGECLAQFPEGVFVQWVCDQYIAPTRAREWTDAGRP
                     PAGPRRHIMRAHMGTYWHRYRDELAIDWIAEMAGRSATHGFDALSIFGESSPFHAGSE
                     LNYLALADYGSDSNPACELSSFLDRVAAPLLGGPERARRFLEIARLIDHREQIPEALA
                     DGRRIAADLSGRPAQRWTWLCNYLASFIYPEPPLGDD"
     gene            complement(1761..>3323)
                     /locus_tag="PLQ54_07890"
     CDS             complement(1761..>3323)
                     /locus_tag="PLQ54_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93218.1"
                     /translation="DLPPPAIWVPAGAHLWETRPAESLGEPILGTPGAPGPVRWSLYC
                     ENGAQATMAYDAATGECRIHCDASGGVGSDIQLIAAPFAVSSNVALQLRFRARASQPF
                     HLPGPVLMKAGPPWTGYSTTRYERIGDVGTEWVEMSRLYGISVNADDGRLTLFLGGAL
                     PAGADLFLADLTLSPCDPSAIPLVDLGNLIFDDEAECGVKVFEPDQLDGQGKYWYDEA
                     RCVVRMWSAGNPGEHYRHIEGALRQHIVDQSGVGYVTYDGLCLKYGGAHGFGGGSTQH
                     ITIRDCDLGYIGGGDQMGGDQTVRFGNGIEFWGAAHDCLVENCRLWQIYDAALTNQNL
                     GSVVEEANITYRNNVIWDSEYSFEYWNRPAESITRNIVFEHNTCVRAGFGWGHTQRPD
                     PSGRHLCFYTSDATASDIIIRDNIFCEAREHAFSAQWWNREQLPSLVMDHNLWFQSEG
                     AVISYADARYTVDQFAAYQAELGLDANSVAAEARFVNADAFDFRLAPGSPGIGAASDG
                     TDMGAAGPVLQP"
BASE COUNT          503 a         1108 c         1175 g          537 t
ORIGIN      
        1 ccctctgcag cggcgccctc gagcacggcg tcgagcagca gtacggtgtt gccctgcgcg
       61 tgcggtgatc ccaggatccc gaggaccttc accatcgcca cctccaatgc gggtgctagc
      121 gcggccgcgt cagtcgtccc caagcggcgg ctcggggtag atgaacgagg ccaggtagtt
      181 gcacagccac gtccagcgct gcgccgggcg gccagacagg tcggccgcga tgcgccggcc
      241 gtcggccagg gcctccggga tctgctcccg gtggtcgatg agccgcgcga tctcgaggaa
      301 ccgccgtgcc cgctccggcc cgcccaaaag gggcgccgcg acccggtcga ggaacgaact
      361 gagctcacac gccgggttgc tgtccgaacc gtagtcggcc agggccaggt agttgagctc
      421 cgatccggcg tggaacggcg agctctcgcc gaagatcgac agcgcatcga acccgtgggt
      481 ggcgctccgc ccggccatct cagcgatcca gtcaatcgcc agctcgtcac ggtagcgatg
      541 ccagtaggtc cccatgtggg cacgcatgat gtggcgcctg gggccggccg gcggtcgtcc
      601 cgcatccgtc cactcacggg cacgcgtggg cgcgatgtac tggtcacaca cccactggac
      661 gaacacgccc tcggggaact gagccaggca ttcgcccgcc cactcggggg cgcccccgcc
      721 gaacccgggg gcctcttcag ggctttcgtg gggctgcgga tgtgagtagg tctcgacgat
      781 gacggtcgcc tcgggccgca ccgaccggat ggcctctgtg gccttggcgt agatcagggc
      841 catgtcctcc cacgagaagc ccgacacggg gtgcctgcgc cgcgccttgc aggtcgggca
      901 ctggcagacg cccgtatcgc cggcctccat ctggacgccg tcgatgtcgg tggtctccat
      961 cagccagcgg agcgactcgg ccgcgtactc ctggttctcg gggcgcgagg ggcacgcgtg
     1021 atggcacagc ttctgcccgg cgaagccgaa gtcgaagatc atccgctcgc ccttggcgtc
     1081 gatcccatag agccccgggt tggccttcag gtgctgctcg agtgaccacg gtgagttgcc
     1141 ctcgtagtaa acgccgccgt aggcgttgag cccgacgccg gccaacagct tggtcccgct
     1201 ctccttggcg acctcgcaga ggcgcttcac cgattcgagc ccgccgtggc ggtccctgag
     1261 cagcccccag acgacgatgc cctcgatgcc gtgcttgccg caccagcgga gcgcagatga
     1321 gtagtcggcg atgaacgatt cggtggtgcg tccgtaatcg ttcgaggcgc ccatgtcgtg
     1381 tccgccggcg cggttgagcg cccatgtggt gcagtggtcc cacgtccaga ggaatcgcga
     1441 tcggatcggt ccctgttctg ccaccgggtc tgcctcctgt gcgtgcccca tcgtgccggc
     1501 gaggccgacc gcgccggccg tcagggcggc agagcggacg aagtcgcggc gggtgatgcg
     1561 atgctcatcg ggtcgttggc tggtgttgcg catacggcca acattcgcca tggggggtac
     1621 gggtggcctg cgtaggacgc gcaagcgtta gcgcagaggc ggcgcgcccc tgtctggcct
     1681 cccagctcgc aggtcgcccc catgcacacg gcctgggtgg agtggggctc agggccaggg
     1741 ctgcgcccgg ctccatggac ctatggctgg aggactggcc cggcggcgcc catgtcggtg
     1801 ccgtcactgg cggcgccgat gccgggggag ccgggggcga ggcgaaagtc gaacgcgtcg
     1861 gcgttcacga accgggcctc ggccgcgacg gagttggcgt cgagcccgag ctcagcctga
     1921 taggccgcga actgatccac cgtgtaccgc gcatcggcgt acgagatcac ggcaccctcg
     1981 ctctggaacc acaggttgtg gtccatcacc agcgagggga gctgctcgcg gttccaccac
     2041 tgggcgctga acgcgtgctc ccgcgcctcg cagaagatgt tgtcgcggat gatgatgtcc
     2101 gacgcggtgg cgtcggacgt gtagaaacac aggtgccgcc cgcttggatc cggtcgctgc
     2161 gtgtgccccc agccgaaacc agcgcggacg cacgtgttgt gctcgaacac gatgttgcgg
     2221 gtgatggact ctgccgggcg gttccagtac tcgaaggagt actcggaatc ccagatcacg
     2281 ttgttgcggt aggtgatgtt ggcctcttcg accacgctgc cgaggttctg gttcgtgagt
     2341 gcggcatcgt agatctgcca gagccggcag ttctcgacca ggcagtcgtg ggcggcgccc
     2401 cagaactcga tgccgttgcc gaagcggacc gtctggtccc cgcccatctg atcgccgcca
     2461 ccgatgtagc ccaggtcgca gtcgcggatc gtgatgtgct gggtgctgcc gccaccgaag
     2521 ccgtgggcgc cgccgtactt gaggcacagg ccgtcgtagg tgacatagcc cacgccgctt
     2581 tggtcgacga tgtgctgccg aagggctccc tcgatgtgcc gatagtgctc gccggggttg
     2641 ccggcggacc acattcggac cacgcagcgt gcctcgtcgt accagtactt gccctggccg
     2701 tcgagctggt ccggctcgaa gaccttcacg ccgcactcgg cctcgtcgtc gaagatcaga
     2761 ttgcccagat caacgagtgg aattgccgag ggatcgcacg ggctgagtgt caggtcggcg
     2821 aggaagaggt cggcccccgc aggcagggcc ccgccgagga acagggtgag ccggccgtcg
     2881 tcggcgttca ccgagatgcc gtagaggcgc gacatctcga cccactcggt gcccacatcg
     2941 ccgatgcgct cgtagcgggt ggtggagtag ccggtccagg gcggcccggc cttcatgaga
     3001 accgggccgg gcaggtgaaa cggctgactg gccctggcac ggaaccgcag ctgcagggcg
     3061 acgttgctcg agacggcaaa cggggcggcg atgagctgga tgtcggaccc gaccccgccc
     3121 gaggcatcgc agtggatgcg acactccccc gtggcggcat cgtaggccat ggtggcctga
     3181 gcgccgttct cgcagtagag cgaccaccgt accgggcctg gggcgcccgg cgtgccgagg
     3241 attggctcgc ccagcgactc ggcaggccgt gtctcccaca ggtgggcgcc ggccggcacc
     3301 catatggcgg gcggcgggag gtc
//
LOCUS       DAOSPL010000365         3319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28826_length_3319_cov_3.293199, whole genome shotgun sequence.
ACCESSION   DAOSPL010000365 DAOSPL010000000
VERSION     DAOSPL010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28826_length_3319_cov_3.293199"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1240)
                     /locus_tag="PLQ54_07895"
     CDS             complement(146..1240)
                     /locus_tag="PLQ54_07895"
                     /inference="COORDINATES: protein motif:HMM:NF033572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
                     /protein_id="HOS93219.1"
                     /translation="MTRVFESLCGQVRIQRPRYRCRRCQASFYAPQRFLDTLGGRRVG
                     PKLLELATQLALYVPYSQASHLLGECLGVSVTGTTLRRDLVQRGEEESWRQGEQAHDC
                     ASFRYEAAPGAPSTARNRQYVEMDGCFVHQWHKKSGFELKVGEMFSDPILTESKKRHW
                     ISRKQYVAHSGSAEAFGERLYAATQPWGLDDAEELYVLGDGASWIKSLWSQYYPHAEF
                     IVDWWHVQNAVWRAVRSLIRDENSRKQWGRRITALLFDGQCQAALSAVERLPASTPQQ
                     IETRQNLSHYLDQNREGLHNYRAVQAQGIQIGSGVIEDACMDVVGRRFKHRGMSWSPR
                     GAEALLCLRTLHLNGQWHHYRHPDALQPAV"
     gene            1806..>3319
                     /locus_tag="PLQ54_07900"
     CDS             1806..>3319
                     /locus_tag="PLQ54_07900"
                     /inference="COORDINATES: protein motif:HMM:NF016450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOS93220.1"
                     /translation="MADTGNGKVIVAPAARDSVAEVPDLIAIQVESYERFLNEGLKEL
                     FETFSPIEDFTGTLSLEFLDYQLGDAKYSVEECRERDITYELPLKAKVRLVTKETGDV
                     LESEVYMGEIPFMTEKGTFLINGAERVVVSQLARSPGVYFKESIDFSGRQLFSAQLIP
                     TEGVWIEFETDANDVIWVKIGQARKFPATTFLRAVNVFDDPDPAHAVECGRDPDLLEL
                     FGERVTLNHPSPEELIGKIAVDEMRDGDRLIVGPYSRIDEAAAEEIAALRRGKFEVLD
                     VNRYIPSTMAQDPTHSDEEAILDIYRKIRPGDPPTKEGAATLCYSMFFDVKRYDLNKV
                     GRHKLNQKLGLNLPLNIRWVTKHDIIAIIKYLLRLSEGAEGYGVDDIDHLENKRIRGV
                     GELLLNQFRVGFLRMERVARERMTSLDPEKLTAQAIISIKPITAAVRSFFGSGQLSQF
                     MDEVNPLSELTHKRRVSALGPGGLSRQSAKLEVRDVHHSHYGRICPIETPEGPN"
BASE COUNT          586 a         1099 c         1004 g          630 t
ORIGIN      
        1 gccgtggtga agggctccgt cccccggcgt gtgccccaag gagtgggctg gacgcctgcg
       61 cccggcgctg cggccctgcg aaggcatgcc cccgtcgcgt cgctgtgtgg cgctgtgtga
      121 ggctgcaaga acttgggtag gtatctcaaa cggcgggttg tagtgcgtcg gggtgccggt
      181 agtggtgcca ttggccgttg aggtggaggg ttcgcaggca caggagcgcc tcagcccctc
      241 gggggctcca gctcatgcct cggtgcttga agcggcgccc gacgacgtcc atgcacgcgt
      301 cctcgatgac acctgacccg atctgaatcc cttgggcctg cacggccctg tagttgtgca
      361 ggccctcccg gttctggtcc agatagtgac tcagattctg ccgtgtctcg atctgctgag
      421 gcgtcgaggc cggcagccgt tcgacggccg acagggccgc ttggcattgg ccatcgaaca
      481 gcagagccgt gatccgtctc ccccactgct tccggctgtt ctcgtcgcgg atcagcgatc
      541 gcaccgcgcg ccacactgca ttctgcacgt gccaccagtc cacaatgaac tccgcatggg
      601 ggtagtattg gctccacagg ctcttgatcc aactcgcacc gtcgcccagc acatacagtt
      661 cctcggcgtc atccagaccc cagggctgcg tcgctgcata gaggcgctcc ccgaaagcct
      721 cggcgctacc cgaatgcgcc acatactgct tccgggagat ccagtgccgc ttcttgctct
      781 ccgtgaggat gggatctgaa aacatctctc cgactttgag ctcaaaacca gacttcttgt
      841 gccattgatg aacgaagcaa ccgtccatct cgacgtattg ccggttccgc gccgtcgacg
      901 gcgcgcccgg ggccgcttcg tagcggaagc tggcacaatc gtgagcctgt tctccctggc
      961 gccacgattc ctcctcgccc cgctgaacca gatcccgccg gagagtcgtg cccgtgacgc
     1021 tcaccccaag acactccccc aggagatggc tcgcttggct gtagggcacg tacagtgcca
     1081 gttgggtcgc caactccaga agcttggggc ccacccgtcg gccacccagc gtgtccagaa
     1141 aacgctgcgg ggcatagaag ctggcctggc accggcggca ccgataccga gggcgctgta
     1201 tccggacttg cccacacagc gactcgaaca cccgagtcac cgagccgccc cggactgacc
     1261 ccccttttta caccgggggc attccgtcgg acgctccttc tctccacccg cttcgactag
     1321 ggcctgtcgt tgagccccct gcgccgcacg gaagatgaga tcctccacct catacgcgtc
     1381 cgcgtcctcg gggatctcca cctcaagcac gacccgtact cggcgacccg ccatccctca
     1441 tcagctcctt ggcttagcct cagagccgat gtacgctccc ctctctcgcg tacctccccg
     1501 tacctaccct ttgcactgca gccccggccc aacagctcta ttgactcagt gatgggacgc
     1561 gggtatatta cctaatcgtg tcgcatagac tgagcagtgt gacccggcag ccgccagctc
     1621 ccccggcgtc cgccgcgagt atgcgtgcgt ttccgaccag gacgtcttcc ccacctgggc
     1681 cgttttcgtt tgtgtgcgcc gccttcatgc gactaccggc acggtaaggg ctcctcttgc
     1741 ccctcggcct ggcgtgggcg cgccgagggc cgtgaacttg gatctgcgag gtgtgttctc
     1801 cgaacatggc agacaccggc aatgggaagg tcatcgtcgc ccccgcggcc agagactccg
     1861 tggcagaagt cccggatctg atcgccatcc aggtcgagtc ctacgagcgg ttcctcaacg
     1921 agggactgaa ggagctgttc gagaccttct cccccatcga ggacttcacg gggactctct
     1981 cgctggagtt cctcgactac cagctgggtg atgccaagta ctcggtcgag gaatgccgcg
     2041 agcgcgatat cacgtatgaa ctgccgctga aggccaaggt tcgcctggtc accaaagaga
     2101 cgggtgacgt gctcgagtct gaggtctata tgggcgagat cccattcatg accgagaagg
     2161 gcacgttcct catcaatggc gccgagcgcg tggtggtcag tcagttggcg cggtcgccgg
     2221 gggtctactt caaggagtcc atcgacttct caggccggca gttgttctcg gcgcagctga
     2281 tccccaccga gggggtgtgg atcgagtttg agaccgacgc caatgatgtg atctgggtca
     2341 agattggcca agcgcgcaag ttcccggcca ccacattcct ccgggccgtc aacgtgttcg
     2401 acgaccccga cccggcccac gcggtcgagt gcggacgtga cccggacctc ctcgagctgt
     2461 ttggcgaacg ggtcacgctg aaccatccga gtcccgagga gctcatcggg aagatcgccg
     2521 tcgacgagat gcgcgacggt gatcgtttga ttgtcggtcc gtactcgcgg atcgacgagg
     2581 cggctgcgga ggagattgct gctctccggc gaggcaagtt cgaggtgttg gatgtcaatc
     2641 gttacatccc cagcaccatg gcccaggatc cgacgcacag cgatgaggaa gccatcctcg
     2701 acatctaccg caagattcgc ccgggtgacc ctcccaccaa ggaaggggcc gccacgctgt
     2761 gctattccat gttctttgat gtgaagcgct atgacctgaa caaggtcggc cgccacaagc
     2821 tcaaccagaa actgggcctc aacctgcctc tcaacatccg ttgggtgacg aagcacgaca
     2881 tcatcgcgat catcaagtac ctcctgcgcc tgagtgaagg cgctgagggg tatggcgtgg
     2941 atgacattga ccacctggag aacaagcgga tccgcggtgt gggtgagctg ctcctgaatc
     3001 agttccgtgt ggggttcctg cggatggagc gggtcgcgcg ggagcgcatg acgagtctcg
     3061 accctgagaa gctgaccgcc caggccatca tcagcatcaa gcccatcacg gcggccgtgc
     3121 ggagcttctt cggcagcggg cagctctcgc agttcatgga cgaggtcaat ccgctgtccg
     3181 aactgaccca caagcgccgg gtctcggccc tgggcccggg cgggctgtcc cgccagagcg
     3241 ccaagctcga ggtccgcgac gtgcaccata gccactatgg gcgtatctgc cccattgaga
     3301 cgcccgaggg gccgaacat
//
LOCUS       DAOSPL010000366         3318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28836_length_3318_cov_3.941771, whole genome shotgun sequence.
ACCESSION   DAOSPL010000366 DAOSPL010000000
VERSION     DAOSPL010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28836_length_3318_cov_3.941771"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLQ54_07905"
     CDS             <1..228
                     /locus_tag="PLQ54_07905"
                     /inference="COORDINATES: protein motif:HMM:NF015748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOS93221.1"
                     /translation="KLGVPVCIGVGGSFDVIAGHVQRAPEWVRRCWGEFLWRIGHDPK
                     RVGRLLAIARFFWQFLLEAPAALRKRRLHGS"
     gene            237..1214
                     /locus_tag="PLQ54_07910"
     CDS             237..1214
                     /locus_tag="PLQ54_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013915186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOS93222.1"
                     /translation="MNLAQVVCLGEALIDFVSQRSGVDLAGAPGFEKAPGGAPANVAV
                     ALARLGTSSAFMGKVGDDPFGRFLADTFAAAGVSTASMVFDPVVKTGLAFVSLMADGE
                     RDFLFYRDPSADMMLRPDEIDETLIRDARAFHFGSITRISEPSRSATTHAIAVARAAG
                     LLISYDPNLRMSLWDTAGHARAEMLAGIPEADVVKVCEEELTFLLGTHDIAAGARALL
                     DMGPSLVAVTRGASGCAVWNHGAHVDLPGFRVDTVDTTGAGDGFVAGALHWLLANAEP
                     SGIAELGEHDLREAFTFANAVGALATTKKGAIPAMPDLATAQSLAATAA"
     gene            1243..2277
                     /locus_tag="PLQ54_07915"
     CDS             1243..2277
                     /locus_tag="PLQ54_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOS93223.1"
                     /translation="MTALLLCCTGLSITAPPARPWRGLHISVGSPEAADDLKRLVVDV
                     LAPMGINTVIAEVNYSFQFQSHPELSNGTLTAEAAADLAATCEGEGVRLIPLFNCLGH
                     QSWADVTFPLLAKHPEFDETPQIPLDNPGIYCRSWCPQHPQVNTVVFELIDEILSAFH
                     ADAFHVGMDEVFLIASDQCPRCRGGDPAALFAQTTNDLHEFLVKRRGVEMLMWGDRLL
                     DAATTGYGQWEASANGTAAAIDRIPKDIIICDWHYEPMADYPSVRYFQEQGFRVWPAT
                     WNKVEAAEALLECALRDPTDRMMGYLFTGWGVNSPDLRDRLQGRTTGKDCPVADVILA
                     CLPRLNANAL"
     gene            2493..>3318
                     /locus_tag="PLQ54_07920"
     CDS             2493..>3318
                     /locus_tag="PLQ54_07920"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOS93224.1"
                     /translation="MAAPRGRRDLARVLIQRGVITEQQAREALERQASDRSQKLERVI
                     VDAGFAPALEVYGVLAELLDVPLANFDNLDIDEDVVNLVPHDVIERVNAIPVKRAPDG
                     GLVFVMAEPDEIEHIDELRRRAGVSVTPMLGVPQQIEEMRGRIYGAVAAATDETDTAF
                     AEIAATRAASGVGAHTAGIGMVADISAIKGTDEEAANTAPIIRIVNTLLKQAIKMGAS
                     DVHIEPQRGNVRIRYRVDGDLREIMTPPKHVHAPLISRMKIMADMDIAEKRVPQDGR"
BASE COUNT          571 a         1163 c         1035 g          549 t
ORIGIN      
        1 aagctaggcg tgccagtgtg catcggcgtc ggcggcagct tcgacgtcat cgcggggcat
       61 gtccagcgcg cgccggagtg ggtgcgcagg tgctggggcg agttcctgtg gcgcatcggg
      121 cacgacccca aacgcgtcgg gcggctgctc gccatcgccc ggttcttctg gcagttcctc
      181 ctcgaagcgc ctgccgcact gcggaagcgg cgcctgcatg gttcttgagg gagtcgatga
      241 acttggcgca ggtcgtctgt ctgggtgagg ctctgatcga tttcgtctcc caacggtcgg
      301 gcgtcgacct ggccggcgcc ccggggttcg agaaggctcc gggtggggct ccggccaacg
      361 tggctgtggc gctggcgcgc ctcgggacct ccagcgcctt catgggcaaa gtcggcgatg
      421 acccattcgg ccgcttcctg gctgacacgt tcgcggccgc tggagtcagt accgcgtcca
      481 tggtcttcga ccccgtggtc aagaccggac tcgcctttgt ctcgctcatg gctgacggcg
      541 aacgggactt cctcttctac cgcgacccaa gcgccgacat gatgctccgc cccgatgaga
      601 tcgacgaaac actcatccgc gacgcccggg ccttccactt tggctccatc acgcggatct
      661 ccgaaccctc ccggtccgcc accacgcacg ccatcgcggt cgcgagggct gccggcctgc
      721 tgatctccta cgaccccaat ctccgcatgt cgctgtggga tacggccggc cacgccaggg
      781 cggagatgct cgccggcata cccgaagccg atgtggtgaa agtgtgcgaa gaagaactca
      841 cattccttct gggcacccat gacatcgccg ccggagcgag agcgctgctg gacatgggcc
      901 ccagcctggt agccgtgacc cgcggagcgt cgggctgcgc cgtctggaat catggggccc
      961 acgtcgatct gcccggcttc cgggtcgaca ccgtcgatac gaccggggcc ggcgacggct
     1021 tcgtcgccgg cgcactgcac tggctgctgg ccaatgccga accgtctggc atcgctgagc
     1081 tgggggagca cgacctacgc gaggcgttca cgttcgccaa cgccgttggc gcgttggcca
     1141 caaccaagaa gggggccatc cccgcgatgc ccgacttggc cacagcccag tcgttggccg
     1201 caacggccgc atagcggccc gcccgcgctc cggaggtgcg acatgaccgc tcttctgctg
     1261 tgctgcaccg gcctctcgat caccgcgccg ccagcgcgcc cgtggcgcgg cctacacatc
     1321 agcgtcggta gccccgaggc cgcggacgat ctcaagcggc tggtcgtcga tgtgctggct
     1381 cccatgggca tcaacaccgt gatcgctgag gtgaactaca gcttccagtt ccagtcccat
     1441 cccgaactat caaacggcac gctgaccgcc gaggcggcgg ccgaccttgc ggccacttgc
     1501 gagggcgaag gcgtccgact gatccccctc ttcaactgcc tgggccacca gtcgtgggca
     1561 gacgtgacgt ttcctttgct tgccaagcat cccgagttcg acgagacgcc acagatccca
     1621 ctcgacaacc ccggcatcta ctgccggagc tggtgccccc agcaccccca ggtgaacacc
     1681 gtggtcttcg agctcatcga cgagatcctc agcgcattcc atgccgacgc cttccacgtg
     1741 ggaatggatg aagtcttcct catcgcgagc gaccagtgcc cccgctgccg aggcggcgac
     1801 cccgccgcgc tcttcgccca gaccaccaac gacctgcacg agttcctggt caagcgcaga
     1861 ggtgtcgaga tgctcatgtg gggcgatcgg cttctcgatg ccgccaccac cggctacggc
     1921 cagtgggaag ccagcgccaa cggaacggca gcggcgatcg accggatccc caaggacatc
     1981 atcatctgtg actggcacta cgaacccatg gccgactatc cctcggtgcg ctacttccag
     2041 gagcagggct tccgcgtatg gccggccacc tggaacaaag tggaggcggc tgaggccctg
     2101 ctcgaatgtg cccttcgcga ccccacggac cgaatgatgg gttacctgtt caccggatgg
     2161 ggtgttaaca gcccagacct gcgcgaccgg ctgcagggcc ggaccaccgg taaggactgc
     2221 ccggtcgccg atgtcatcct ggcgtgcctg ccccggctga acgcgaacgc cctatagctg
     2281 cagatgggtg ctgatgaggt gccggccgcg cccccctgca tgtgctataa tcgctcctgc
     2341 ccacggccgc gaggctgctc gcttgccatt tggggcatcc agtccaagga gttcgcccat
     2401 cggacacaga aagtgctcgc cgctgaacac gtatgcgccc gcggaagctc tgatgctgtc
     2461 cgcgttgaac tgtatacctg gaggtgccca ggatggctgc gccacgtggc cggcgtgacc
     2521 tggctcgcgt cctgatccag cggggcgtca tcaccgagca gcaggcgagg gaggcactgg
     2581 agcgccaagc ctccgaccga tcccaaaaac tcgaacgcgt catcgtcgat gcgggattcg
     2641 cgccagccct cgaggtctac ggcgtgctcg ccgagctcct cgatgtcccc ttggcgaact
     2701 tcgacaacct cgacatcgac gaggacgtgg tcaatctcgt cccccatgat gtgatcgaac
     2761 gcgtcaacgc catacccgtc aagcgcgctc ccgacggggg cctcgtcttc gtcatggccg
     2821 agcccgacga gatcgagcac atcgacgaac tccgccggcg cgccggcgtc agcgtgaccc
     2881 ccatgctcgg ggtgccccag cagatcgagg agatgcgcgg ccgaatctat ggcgctgtcg
     2941 ccgccgctac cgacgagacg gacaccgcct tcgccgagat cgccgcaact cgggcggcct
     3001 ccggcgtcgg ggcccacacc gccggtatcg gtatggtggc cgacatcagc gccatcaaag
     3061 gcaccgacga ggaagcggcc aacaccgcgc ccatcatccg cattgtgaac accctgctca
     3121 agcaggccat caagatgggt gccagcgacg tccacatcga gccccagcgc gggaacgtgc
     3181 gaatccggta ccgcgtggat ggtgaccttc gtgagatcat gacaccaccc aaacacgtcc
     3241 acgcgcccct gatctcgcgc atgaagatca tggccgacat ggatatcgcc gagaaacgag
     3301 tcccgcagga cggccgtg
//
LOCUS       DAOSPL010000367         3318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28843_length_3318_cov_2.690469, whole genome shotgun sequence.
ACCESSION   DAOSPL010000367 DAOSPL010000000
VERSION     DAOSPL010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28843_length_3318_cov_2.690469"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1804
                     /locus_tag="PLQ54_07925"
     CDS             <1..1804
                     /locus_tag="PLQ54_07925"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93225.1"
                     /translation="LAAIEALHRAGILTPEQSTHLSRGYVLLRRIEHALQIVQESRET
                     VLPADAQARAILGARLGFASLASFDHAYREATRAVRGAFEALFLGDLGATFAEHTAIQ
                     RWIRGDEELAAEARAALEAAGLRDVDAAQARLVELSGTTPDSRERLSAIVEELLVEAS
                     GSGDPDACIANLARIVRSTRAPASLYWFLYDYPAAIHRLCYVCGRSDVLSELMARRPE
                     LMDAMLEPGALDSPKSRAALRQELAQRLSFERRHEGRLRAIRLFRARESLRIGLRDLT
                     MDVRLEDTLAALSDLAEVCLDASLEVEARELHAPEPHGGLAILGLGKLGGRELHYQSD
                     LDVVFVAATDDAAGYDRAYAVARRLMQDLPARAPEGMIYAIDPRLRPGGREGPLVATL
                     AGLRTYYGTRGHEWERLASVRARFVCGDPIVGQAALEAIREFAYSGEWTPEEKAEIHR
                     IRGLIAREKGRPAPGEFDVKLGPGGTLDLEVAVQAIQAETGSTDPSVRVPSTLAALAA
                     LGRSGALDPESASAAIAAYRLLRIVESRLRILRERSTSRVSLAEEDLHVLAAKLPLEG
                     RHKPMTGTDLAAELGHASQAARRLYRTAIAELDW"
     gene            1907..2497
                     /locus_tag="PLQ54_07930"
     CDS             1907..2497
                     /locus_tag="PLQ54_07930"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS93226.1"
                     /translation="MARPDSIASVAPTRVEALAHRAARGDVDAFDELVSLHAGKVVNL
                     AFRLLGDATDADDVAQEAFVKAYRSLPRLRDRSQFTAWMLKITTNACRDYLRRRGRRD
                     EVGLDEAINHAGGDATAHVVEQQETQARIRHLMNTLPQKHQIVLYLRDIEGLSYDEIA
                     TTLGCTVSSVKNRLHRAREAFRIRAMPYVDEVLPWE"
     gene            2509..3315
                     /locus_tag="PLQ54_07935"
     CDS             2509..3315
                     /locus_tag="PLQ54_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOS93227.1"
                     /translation="MSRTEFQGFAALVDEYMDGELDPRLRAACEAHVASCPECGQWLA
                     RARRAQELLASGPRVEPGRGFRLALRGRLEVEQLRRRRRERVIRSAAAFLAPATVAAL
                     VLVQHMPAGRDRLPEASRVTASAAHGPTTGPGTPWMAPPQTADTGAAMVRGADPAPVP
                     RLVVPSVNTPRSRRGVAPHIPKPTPAAASPEGPTTSSDSGDGLDSVLEAVPAPSVHAP
                     IQFAADRHSPSYVTPALAQTAPATPAPPLVRPLPAPDGVFAAGRDTTYEV"
BASE COUNT          518 a         1197 c         1120 g          483 t
ORIGIN      
        1 cctggcggcc atcgaggcgc tccaccgcgc cggtattctc acacccgagc aatcgaccca
       61 cctttcccgc ggttacgtgc tcctccgccg gatcgagcac gcgctgcaga tcgtgcagga
      121 gagccgcgag acggtcctcc ctgcggacgc gcaggcccgg gccattctcg gtgctcgact
      181 cgggttcgcg tcgctcgcca gtttcgacca tgcataccgc gaggcgacgc gagcagttcg
      241 aggagccttc gaggccctat tcctcggtga ccttggcgcc acattcgcgg agcacacggc
      301 catccagcga tggatccgcg gcgatgagga actggcggcc gaggctcggg ccgccctcga
      361 ggctgctgga ctcagagacg tcgatgcggc ccaggcgcga ctggtggaac tgtccggcac
      421 gacacccgac tcgcgggagc gactgagcgc catcgtcgag gagctgctgg tcgaggcctc
      481 gggatccggt gacccggacg cgtgcatcgc caacctcgcg cgcattgtac ggtcgactcg
      541 cgcccccgcc tcgctgtact ggttcctata tgactaccct gcggcgatcc accgtctctg
      601 ctatgtgtgc ggacggagcg atgtgctctc cgagctaatg gcccggcgcc cggagctgat
      661 ggacgccatg ctcgaacccg gcgccctcga tagccccaag tcgcgcgctg ccctgcgtca
      721 ggaactcgcc cagcggctgt ccttcgagcg gcgccacgag gggcgtctgc gcgcgatccg
      781 cctcttccgg gccagggaaa gcctgcggat cgggctccgc gacctgacca tggacgtgcg
      841 actcgaggat accctcgccg cactgagcga cctcgccgag gtgtgcctcg atgcatccct
      901 cgaggtcgag gcgcgggagc tgcatgcacc cgaaccccac gggggcttgg cgatcctggg
      961 actgggcaag ctcggcgggc gtgagctcca ctaccagtcc gatctggacg tggtgttcgt
     1021 ggcagccacg gacgacgccg ctgggtacga cagggcctac gccgtcgcgc gccggctgat
     1081 gcaggacctg cccgcgcgtg cccccgaggg catgatctat gccatcgatc caaggctccg
     1141 gcccggggga agagagggcc ctctcgtggc aaccctggcc ggcctgcgga cctactatgg
     1201 cacgcgcgga cacgagtggg aacgcctggc ttcggtgcgc gcccgattcg tgtgtggtga
     1261 tcccatcgtc ggtcaggcgg ccctcgaagc cattcgcgag ttcgcatatt cgggcgaatg
     1321 gaccccggaa gagaaggccg agatccatcg catccgcggc ctgatcgccc gggagaaggg
     1381 acggccggcg ccaggcgagt tcgatgtgaa gctcgggccc gggggaaccc tggacctgga
     1441 ggtggccgtc caagccatcc aggcggagac cggttccacg gacccctcgg tacgagtgcc
     1501 gagcactctg gcggccctcg cggccctggg gcgtagcggg gccctcgacc ctgagagcgc
     1561 gtcggccgcc atcgcggcct accggctgct gcggatcgtg gagagccggt tgcggattct
     1621 gcgggagcga tcgacgagtc gggtctcgct ggccgaggag gatctgcacg tcctggcggc
     1681 taagcttcct ctggaggggc ggcacaagcc gatgaccggg accgacttgg ccgccgaact
     1741 cggacacgcg tcgcaggcgg cgcgccggct ctaccgcacg gcgattgccg agctcgactg
     1801 gtgagatacg tgagactcct ccacggccgg aagcgtctac cctgcaaaag gcgggaagcg
     1861 cgtcccggaa gggagtggcc cgtgcatggc gctggaggcc ataggggtgg cgcgccctga
     1921 ctcgatagcc tcggttgccc ccacgcgcgt ggaggcgctg gctcaccgtg cggctcgcgg
     1981 cgacgtcgac gccttcgacg agctcgtctc actgcacgcc ggcaaagtgg tgaatctcgc
     2041 ctttcggctg ctcggcgacg ccacggacgc cgacgacgtg gcccaggagg ccttcgtcaa
     2101 agcctatcgg tccctgccgc gcctcaggga ccggtcccag ttcaccgcct ggatgctgaa
     2161 gatcacaacc aacgcctgcc gcgactacct ccggcggcgc ggccgccgcg acgaggtcgg
     2221 gctcgacgag gccatcaacc acgcgggcgg cgacgccacc gcccatgtgg tcgagcagca
     2281 ggaaacgcag gcgcgcattc gccacctgat gaacacgctc ccccagaaac accagatcgt
     2341 gctctaccta cgtgacatcg aagggctctc atacgacgag atcgccacca cgctcggatg
     2401 cacggtctcg agcgtgaaaa accgcctgca ccgagcccgc gaagccttca ggatccgcgc
     2461 catgccctac gtcgatgaag tgcttccctg ggagtgatga ctgagacaat gagccgcaca
     2521 gagttccagg gctttgccgc cttggtagac gagtacatgg acggcgagct ggacccgcgc
     2581 ctgcgagcgg cgtgcgaggc ccacgtcgca tcatgccccg agtgcgggca gtggctggcg
     2641 cgggcacgcc gagcccaaga gctcctggcc tctggcccaa gggtcgagcc ggggcgcgga
     2701 ttccgcctgg ccctccgcgg acgcctcgag gtcgagcagc tccgccggag acgccgggag
     2761 cgggtcatcc gatcggccgc cgcgttcctc gcgccggcaa cggtggccgc cctggtgctc
     2821 gttcagcaca tgccagccgg ccgcgaccgg cttcccgagg cgtcgcgggt cacggcctct
     2881 gcggcgcacg gccccacaac gggccccgga accccgtgga tggcgccacc ccagacggcc
     2941 gacaccggcg cggccatggt ccggggcgcc gacccggctc cggtgccacg gctcgtcgtt
     3001 ccctcagtga acacgccccg ttcgcgaaga ggcgtcgcgc cccacatccc caaacccacg
     3061 ccagcagccg cctcccctga gggaccgacc acctcctccg actcggggga cggcctggac
     3121 tccgtactcg aggcggtccc ggcaccgtcg gttcacgccc cgatccagtt cgccgccgat
     3181 cgccactcgc cgtcctatgt gaccccggcg ctggcccaga cggcaccggc gacaccggca
     3241 cccccactcg tccggcctct cccggctccc gacggcgtgt tcgccgccgg gagggacacc
     3301 acgtacgagg tctgaagg
//
LOCUS       DAOSPL010000368         3316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28865_length_3316_cov_3.034039, whole genome shotgun sequence.
ACCESSION   DAOSPL010000368 DAOSPL010000000
VERSION     DAOSPL010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28865_length_3316_cov_3.034039"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PLQ54_07940"
     CDS             <1..671
                     /locus_tag="PLQ54_07940"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOS93228.1"
                     /translation="LLMALIWGRKDRVRQAEAVLQYAPSAGGPRRLPLVFAFLLALMI
                     YGGIEMPTLPKVVGMVALALGLALTAAKSMDRDELMSWLRETGLLVKLVVPVLVPAVL
                     IIGAIAYYTPILFIDKYLGYRNPFATVYSAVFGSLMYFPILTEVAFVKAFLKNHMDVG
                     PAFALLLTGSGLSLPGMILIGRAMGAGRVVTYVLIVLALVLVTSAIFAQSIGAYICPC
                     QSGP"
     gene            765..1745
                     /gene="modA"
                     /locus_tag="PLQ54_07945"
     CDS             765..1745
                     /gene="modA"
                     /locus_tag="PLQ54_07945"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOS93229.1"
                     /translation="MRRLAFVVAGVLVVGSLAGCPSKAPDDGGGVTAATGGKGAQVAK
                     AGGAAPGGALTGKLVLYIPCGLTIPMKAVVQAFKAANPGLEVTEEYDSAVVLARRIID
                     KKERPDVLVSPGPTELGLVAEKGLIDTANQKSLGSFKLCVIAPKANKAKMVAIEDLAR
                     VKSISCPDPKLNSVGAAAQEALTNMGLWDKIQKQLVFAEIGHESHNHVAAGRVDAGIS
                     FLACPLDTNPEKLSKSKVTIVGVFPDGTYKEPSVEVAPLTEAKNPAAAKAFVEYLVSP
                     EAQKILSEKELPGLSAETTGEGWPKLKAPEGTAADKASPAAKGEAKGAGE"
     gene            1790..3037
                     /locus_tag="PLQ54_07950"
     CDS             1790..3037
                     /locus_tag="PLQ54_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOS93230.1"
                     /translation="MKRWLPVVLLAGAMVLLAGCKGRGDSEEPQAAMPKAPTKATKAV
                     AAGELGPGVYKLDGFVCVTAFYPGNEDHQWVRDYLKQLEDKHPGKVKTRFVAFNTDEG
                     ATEWQEAGLECGTWFIDGESEVTFTQDGKEKTVWFVKEEGGEWSEADLFAAVADRVSK
                     LSAGGAAASSTGPLSGTVVVLVPCGMQGPYGKARALFKESHPDLKVEQDVQNIEVLIH
                     GIVSGKFTGDIVLSMGDRETAFLNDKGKLARDPVRLARNTLGMIVPNSNPAGIRGFKD
                     LANAKVKTLGLPDPELISAGYYGKQALEKAGLWESLAKRVSLVKSPAALKGDLASGKM
                     QAAITFGTCMTEAKTVGGEPTKSDKTDAIDYVPQEYYDEFYCTGAVLKSAKNPSAAEA
                     FLQFMTTEPVQKIFAEYGLKTIE"
BASE COUNT          578 a          929 c         1239 g          570 t
ORIGIN      
        1 ggctgctgat ggcgctgatc tggggccgca aggaccgagt ccgccaggcc gaggcggtgc
       61 tgcagtatgc gccgagcgct ggcgggcccc ggcggctccc cctggtgttc gcattcctgc
      121 tggcgctgat gatctatggc gggatcgaga tgccgaccct gccgaaggtc gtcgggatgg
      181 ttgccctggc gctcgggttg gcgctcacgg cggcgaagag catggaccgc gacgagttga
      241 tgtcctggct gcgcgagacg ggcctgctcg tgaagctggt ggttccggtt ctggtgccgg
      301 ccgtgttgat catcggggcc atcgcctact acacgccgat cctgttcatc gataagtatc
      361 tcggctatcg gaacccgttt gccacggtct attcggccgt cttcgggtcg ctaatgtatt
      421 tcccgattct aacagaggtc gcgtttgtaa aggcgtttct caagaatcat atggatgtcg
      481 ggcccgcgtt cgcgctgttg ctgacgggtt cggggcttag cctgccgggg atgatcctca
      541 tcggccgggc gatgggcgcg gggcgcgtcg tcacctatgt gctgatcgtg ttggccctgg
      601 tcctggtcac ctcggcgatc ttcgcccagt cgatcggcgc gtacatctgc ccgtgtcaga
      661 gcgggccgtg atgtaggggc accgcaggtg cgctacaatc gtgaggttat ggccggtagc
      721 ccttgggttg cgcactcatt cgagactaat gaggagagag actgatgcgg cgattggcgt
      781 tcgttgtggc cggagtgctc gtggtcggat ctctggctgg ctgtccgagt aaggcgccgg
      841 acgacggtgg cggggttacg gcagccaccg gaggcaaggg ggcgcaggta gccaaggccg
      901 gtggcgcggc gccggggggg gctttgaccg ggaagctggt tctgtacatt ccatgcggtc
      961 tcaccatccc gatgaaggcg gtggtgcagg ccttcaaggc ggctaacccc gggctcgagg
     1021 tcacggagga gtacgatagc gccgtggtgc tggcgcggcg aatcatcgac aagaaggagc
     1081 ggcccgacgt cctggtgtcg cctgggccga cggagttggg gttggtggcg gaaaagggcc
     1141 tgatcgatac cgccaatcag aagtcgctcg ggtccttcaa gctgtgcgtg atcgccccga
     1201 aggcgaacaa ggcgaagatg gtcgccatcg aggacttggc tcgggtgaag tccatctcgt
     1261 gccccgatcc caagctcaac tccgtgggtg cggccgccca ggaggccctg acgaacatgg
     1321 ggctatggga caagatccag aagcagttgg tcttcgcgga gatcggccac gagtcgcaca
     1381 accatgtggc ggcagggcgg gttgatgcgg gcatctcgtt cctggcgtgc ccgttggaca
     1441 cgaaccctga gaagctctcg aagagcaagg tgacgattgt gggcgtgttc cctgacggca
     1501 cctacaagga gccgtcggtg gaggtggcac cgctgacgga ggccaagaac ccggcggctg
     1561 ccaaggcgtt cgtcgagtat ctggtcagtc cggaagcaca aaagatcctg tcggagaagg
     1621 agcttccggg tctcagtgct gagacgaccg gcgaggggtg gccgaagctg aaggcccccg
     1681 agggcacggc ggcagacaag gcaagtccgg cggcgaaggg tgaggcgaag ggtgccggcg
     1741 aatagccggg tctgatcgac cgtagcgatg ggaggcgttc tgtttgccga tgaagcgttg
     1801 gctacctgtg gtgctcctgg ccggagcgat ggtgctccta gccggctgca aggggcgtgg
     1861 cgactccgag gagccgcagg ccgctatgcc caaggccccg acgaaggcga caaaggcggt
     1921 tgcggcgggc gagctcggcc ccggtgtcta caagctcgat ggtttcgtgt gcgtgacggc
     1981 attctacccg ggcaatgagg accaccagtg ggtccgggac tacctgaagc agctcgagga
     2041 caagcacccg ggcaaggtca agacccggtt cgtggcgttc aacacggacg agggcgccac
     2101 ggagtggcag gaggcggggc tcgagtgcgg gacgtggttc atcgacggcg agtcggaggt
     2161 cacgttcacc caggacggca aggagaagac ggtctggttc gtcaaggagg agggcggcga
     2221 gtggagcgag gccgacctgt tcgcggccgt ggcggatcgg gtgtcgaagc tctcggcggg
     2281 cggcgccgcg gccagttcga cgggtccgct cagtggcacg gtggtggtgt tggttccgtg
     2341 cggtatgcag gggccgtatg ggaaggctcg ggccctgttc aaggagtcgc accccgacct
     2401 gaaggtcgag caggatgtgc agaacatcga ggtgctcatc cacggcatcg tctctgggaa
     2461 gttcacgggc gacatcgtgc tctcgatggg cgatcgcgag acggcgttcc tcaatgacaa
     2521 gggcaagctg gcgcgcgacc cggtgcggtt ggcccggaac acgctgggga tgattgttcc
     2581 caacagcaac ccggctggta tccggggctt caaggacctc gccaacgcga aggtcaagac
     2641 gctgggctta ccagaccccg aactcatttc ggcgggctac tacggcaagc aggcgctcga
     2701 gaaggccggc ctgtgggagt cgctggcgaa gcgcgtatcg ctggtgaagt ccccggcggc
     2761 gctcaagggc gacttggcgt cgggcaagat gcaggcggcc atcacctttg gcacctgcat
     2821 gacggaagcg aagactgtgg ggggcgagcc gacgaagtcg gacaagaccg acgccatcga
     2881 ctacgtgccg caggagtact acgacgagtt ctactgcacg ggtgccgtgc tcaagtcggc
     2941 gaagaacccg tcggcggcgg aggcgttcct ccagttcatg acgaccgagc cggtgcagaa
     3001 gatcttcgcg gagtacgggc tgaagacgat cgagtagcga ctgggctcag gaatgaggaa
     3061 tcaggggtca cggtgccata ggcgctaaca tagggccctt ggccgggcgg ctgggtctcg
     3121 cgaaggtcgc gcggaacgcc cgcctggtct tgcggagggc cgcacagagg tgcgcccaga
     3181 gggcgcgggg cccctgtcct gagcctgtcg aaggacccag tcggccggac gacgacagag
     3241 cggcccggga tgcggcagca agccgcatcc cggcctccag atcgggcgcg ttgggggccc
     3301 accacgctgg gagacg
//
LOCUS       DAOSPL010000369         3311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28921_length_3311_cov_3.682125, whole genome shotgun sequence.
ACCESSION   DAOSPL010000369 DAOSPL010000000
VERSION     DAOSPL010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28921_length_3311_cov_3.682125"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PLQ54_07955"
     CDS             complement(<1..696)
                     /locus_tag="PLQ54_07955"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOS93231.1"
                     /translation="MAQPLDDGLGAAVRAALRAPPGQMDMGALRSLAARCPALRCLRD
                     CLGVIQGGHHRFDVFEHSVHALTRLEEVSSRLIEVFPATHGAVEAYLRAPGRRSALRL
                     ATLLHDVGKPARRTPGQGRYRFFGHEHESARLVEDAAAGLGFGVVGLRTVVLLVRNHM
                     RPPNLADMRDDGTLTERALRRLGRDMGSHLPGLMALFIADAAATRDLPILGPELAGAR
                     ELAEQALAARAAPA"
     gene            951..1520
                     /locus_tag="PLQ54_07960"
     CDS             951..1520
                     /locus_tag="PLQ54_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93232.1"
                     /translation="MGTAVADEPTPQEGPKVPPEAPRETDRRRGARVEWLHKDSGQRF
                     LGPKRWVWRDPDNHFKVNKIAMPPDARSYMPCVILRIKPLDKGVTFPMIVKYAEYRLR
                     KLIDVDKVIENAPTTLPFENQGAHVLHVTGKIERRGTPVECRQIVYLRGQWFTVIACG
                     SALGDFGDHQVEFDELISGITFDPPKPGR"
     gene            complement(1590..2738)
                     /locus_tag="PLQ54_07965"
     CDS             complement(1590..2738)
                     /locus_tag="PLQ54_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93233.1"
                     /translation="MLAALLCRSAFVAQGGTDVVTRTVVQTEDRLARRRGVSPLDLRQ
                     HMRRHLGMVVPVYFPEDFDPVQTQDLLLATLADTELFVDPSRQVLVLDGVRWVNETAR
                     RVADQVERRAGAPLRVVCLDDNRGKGGAVCAGMIELLADPAVTHIGIRDDDRDHAIWD
                     LPELYALSLQMAEDEATDLTLANGRRAEIHRPLGMARGFLEALVGEVTYDAVRFALAR
                     ENRALNTRWHSAYGPVPDMESGYKIYSRRAAGLMIHGIQAAATRLPSLNLGHFGVEIV
                     PTVEVLLAGGIVGEVMRTTCQAQPLTTFAAGTRLPQLFGAELVWLFTRLDTPAHVADT
                     LLLNAQARCGYGQCSEGRQALAELRAFVLDHLGVPEAQRPEVRLPERF"
     gene            complement(2958..3143)
                     /locus_tag="PLQ54_07970"
     CDS             complement(2958..3143)
                     /locus_tag="PLQ54_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93234.1"
                     /translation="MPSKYQVKYDQASRTFGVWRWTDLPRRAQPVAQQFRRESDAYEW
                     IRQNTTREDKVYPTLFN"
BASE COUNT          566 a         1077 c         1165 g          503 t
ORIGIN      
        1 ggcgggggcc gcccgcgcgg ccagtgcctg ctcggccagc tcgcgggccc ccgccagctc
       61 gggaccgagg atcggcaggt cgcgggttgc ggccgcgtcg gcgatgaaga gggccatgag
      121 gcccggcagg tgcgaaccca tgtcccggcc gagccggcgc agggcccgct cggtgagggt
      181 gccgtcatcg cgcatgtcgg ccaggttagg cgggcgcatg tggttacgga cgagtagcac
      241 cacggttctg agccccacca caccgaagcc tagtccagcc gccgcgtcct cgacgagccg
      301 agcgctctcg tgctcgtggc cgaaaaaccg gtagcggccc tggccgggcg tgcggcgcgc
      361 cggcttcccg acatcgtgga gcagggtggc gagccgcaga gccgaccggc ggcctggggc
      421 acggagatag gcctccacgg ccccatgcgt tgcggggaac acctcgatca gccgcgagct
      481 tacctcctcg aggcgggtca gggcgtgtac cgagtgctcg aagacatcga agcggtggtg
      541 accgccctgg atgacgccga ggcagtcgcg aaggcagcgc aatgcgggac accgggcggc
      601 gagggatcgc agcgcgccca tgtccatttg cccaggcggg gcgcgcagcg cggcgcggac
      661 tgcggcgccc agtccgtcgt cgagaggctg tgccattcga tctccctcac tgctgtactg
      721 gtgggaactg cggctgcccg gcgggcattg acacgagcgg cgccgcaact gacgatgtgc
      781 actgcgccac cggtgtccag atcccattat cagccggcgg gtgcatcgga acaacgaagg
      841 ggatggtagg agtgcgcgtt gtgacggttt gggggatctc ggccgccgtt gtggtggcat
      901 tgtccgcatg ttgggcgcaa ggcccttctg tcatggcggg cggcaggtct gtgggaacgg
      961 cggtggccga tgagccgacc ccgcaggagg ggccgaaggt tccgcccgaa gcgccgcgcg
     1021 agaccgatcg gcggcgggga gcccgggtgg agtggctcca caaggacagc ggccagcgtt
     1081 tcctgggacc gaagcgttgg gtgtggcggg atccggacaa ccatttcaag gtgaacaaga
     1141 tcgcgatgcc gccggacgcc cgcagctaca tgccgtgcgt gattctgcgc atcaagccac
     1201 tcgacaaggg cgtcacgttc ccgatgatcg tgaagtacgc cgagtaccgg ctgcgaaagc
     1261 tcatcgatgt agacaaagtg atcgagaacg cgccgaccac cttgccgttc gagaaccagg
     1321 gcgcgcacgt gcttcacgtc accgggaaga tcgagcgccg cggaacaccg gtggagtgtc
     1381 ggcagatcgt gtaccttcgg ggccagtggt tcacggtcat cgcgtgcggg agcgcgctcg
     1441 gggacttcgg cgatcaccag gtggaattcg acgagctgat cagcgggatc acgttcgacc
     1501 caccgaagcc gggaaggtag gcgatgggtc ccggggacac ccaacacagc cggcgcacct
     1561 ctggtgcgga gtgaggtgtc gccgagggat cagaaccgct cgggcaggcg cacctccggc
     1621 cgctgggcct cgggcacgcc caggtggtcg aggacgaagg cccgcagctc ggctaacgcc
     1681 tgacgccctt cgctacactg accatatccg cagcgtgcct gtgcgttcag cagcagcgta
     1741 tcggccacat gggccggggt gtccagccgc gtgaacagcc agaccagctc ggcgccgaag
     1801 agctgcggga gtcgagtgcc ggcggcgaac gtggtcaggg gctgggcctg gcaagtggtc
     1861 cgcatcacct ctcccacgat gccgccggcc aagagtacct caaccgtggg gacgatctcg
     1921 acaccgaagt ggcccaagtt cagcgaaggc agccgggtgg cggcggcctg gatgccatgg
     1981 atcatcagcc cggccgcacg acgagagtag atcttgtagc cgctctccat atcgggcacg
     2041 ggcccgtaag ccgagtgcca tcgggtgttg agggcacggt tctcgcgggc cagggcgaac
     2101 cgcaccgcgt cgtatgtcac ctcgcccacg agcgcctcga gaaacccacg ggccataccc
     2161 agtggacggt ggatctcggc gcgcctcccg ttggccagag tcaggtcggt cgcctcgtcc
     2221 tcggccatct gaagggagag ggcgtacagc tcgggcagat cccagatggc gtggtcacgg
     2281 tcgtcatcgc gaatgccgat gtgggtcacg gccggatcgg cgagtagctc gatcatgccg
     2341 gcgcagacgg cgccgccctt gccgcggttg tcgtcgagac agacgacccg cagcggagcc
     2401 ccggcccggc gctcaacctg gtcggccacc cggcgcgccg tctcgttgac ccaccggacg
     2461 ccgtcgagga ccagcacctg gcgtgagggg tcgacgaaca gctcggtgtc ggcaagggtg
     2521 gcgagtagca gatcctgggt ttgcaccgga tcgaagtcct cgggaaagta cacgggcacc
     2581 accatgccca ggtgccgcct catgtgctgc cgcagatcca gagggctcac tcctcttcgg
     2641 cgcgccaatc ggtcctccgt ttggaccacc gtccgcgtga cgacatcggt gccaccctgg
     2701 gcaacgaagg cggaccggca cagaagcgcc gccagcatag gcatactccc agccagtgtg
     2761 aacgggcagg agagaggcac atctgctgaa cggttcgtga ttcgacggca gcggaccagg
     2821 gcctgctcgc ctgcaagctg gcggcggctc agcagacagc catcggcaag acgagggccc
     2881 ggccacaaga tgggccgggc ccagcccgtg cagtcatgcg accgtggcag tcaagtgtgc
     2941 ggcgcacgag gggaggttca gttgaacaga gtagggtaga ctttgtcctc gcgcgtggtg
     3001 ttctggcgga tccattcgta cgcgtcactc tcgcgccgga actgctgagc caccggctga
     3061 gcccgacggg gcagatcggt ccaccgccac acaccgaatg tcctggaggc ctggtcgtac
     3121 ttgacctgat acttgctggg catcgtccca ccccattccg cttctcacat tcccccgctc
     3181 ctggccgact cccgcatgca gtattccccg atctcgcggc ttccaaaccc cgggcgcggg
     3241 ttccacgggt atggaatctg gtaaccaacc cttgccgccg ctttgcggca catgtacgct
     3301 ggcctcggtc c
//
LOCUS       DAOSPL010000370         3309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28944_length_3309_cov_2.935464, whole genome shotgun sequence.
ACCESSION   DAOSPL010000370 DAOSPL010000000
VERSION     DAOSPL010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28944_length_3309_cov_2.935464"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(482..1843)
                     /locus_tag="PLQ54_07975"
     CDS             complement(482..1843)
                     /locus_tag="PLQ54_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOS93235.1"
                     /translation="MSASIRYLLPPSDMPSAWYNVLPRLSTPLAPPLNPATGQPAGPA
                     DLLPIFPEGLVMQEMCPDPWVDIPGPVLDIYRTYRPSPLVRAIRLEEALETPAHIYYK
                     YEGVSAAGSHKPNTAIAQAYVNKQEGVRRLATETGAGQWGSALAHACAMFGLECTVYM
                     VRVSYNQKPYRRSFMQVFGAAVHSSPSTVTEIGRKVLADDPDTPGSLGIAISEALEDT
                     VKHEDCKYSLGSVLNHVLLHQTIIGLEAKQQLEKLGEQPDVVIGCHGGGSNFAGIALP
                     FVDDKLAGRTVRLLAVEPTACPTLTRGPYAYDFGDLGQMTPLLKMHTLGHSFVPAGIH
                     AGGLRYHGAAPICSKLLADGMIEAKAYPQVPVFDAAVQFARAEGLVPAPESAHAIRAV
                     VDEALEAKELGEERVILFNLSGHGMFDLGAYDAYFAGELVDYDLPAEALDRALKCLPA
                     VGG"
     gene            2050..3222
                     /locus_tag="PLQ54_07980"
     CDS             2050..3222
                     /locus_tag="PLQ54_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93236.1"
                     /translation="MRRATVWVIAVGALSFPAWPQSPEVLAEVEAVIGPWRVAAPADN
                     GFADLVAAIQRVTEGDSVDLLNGPDAGPAAEELIATNAEALAAVRAAMGRECRVPPVT
                     SFTTPLPYLANARVTARVLCVEGRCREVDGDPSGALGSYLDAVQLGGAISRGGTLIHG
                     LVDAAVIRIASAEVRRLCESGPAFPDATLADAIDRLTRLERAGPSPQQFLATELEASV
                     AGLREIAENPQLLEQIATDAGMSRSRGTIELDAEARALIEYYGRLIELLDLPYRDSLG
                     VAQPPEAAETPLAAMVAPSADRASERLRGGAAVLRGVRVLLAIVRFRQAREAYPDTLS
                     QLVPDYLPAAVVDPYTLEPFVYAGTPNGRALYSPGPDGVDDGADEERDVVIAALPE"
BASE COUNT          521 a         1171 c         1080 g          537 t
ORIGIN      
        1 gaagggcgtg gtagccgtcg gcaagccgga tcagcagcac gggcatgcgg ccgaccttga
       61 cagaccgcat ctgcccgacg gccatctcgc cctgctccgc gactcgctgc tccatgatgc
      121 ctgccccttc cgtcgtcgtc cgggcggacc cgccgcccgc accgcggcga ggcccacggc
      181 ccccaggccg cccagcacgc aggcagtggt ccctcgcacg cctaggctcg tcccctgaca
      241 cggggccatc gcggcccggc cgggccggcc gtctcaagtt gcggtgtgcg cacgggctgt
      301 tgctccgcga catggtgact tcagagagac gtgacctccg acgccctcgg cgcttcgcgt
      361 gcccgtgcgc cgcaacctgc acgcacaagg gcccgacctg ctgctccccc acggcgagcc
      421 ggggcggccc gacttcccga gccgccccca aaaaccagcg cgccggtctc gacttcccgc
      481 tctaccctcc cacagccgga aggcacttca gcgcacggtc gagggcctcg gcgggaaggt
      541 cgtagtccac cagctcacct gcgaagtagg cgtcgtaggc acccagatcg aacatgccgt
      601 gcccgctgag gttgaacagg atcactcgct cctcgcccag ctccttggct tccagcgcct
      661 cgtccaccac cgcacggatg gcgtgagcgg attccggcgc cgggacgagc ccctcggccc
      721 gcgcgaactg caccgcggca tcgaacacgg ggacttgtgg gtatgccttg gcctcgatca
      781 tcccgtcggc cagcagcttg ctgcagatcg gtgccgctcc gtgatagcgc agcccacccg
      841 catgaatgcc ggccggtacg aagctgtggc ccagggtgtg catcttcagc agaggcgtca
      901 tctgacccag atcgccaaag tcgtaggcgt agggcccgcg agtcagggtc ggacaagccg
      961 tcggctccac ggcgagcagc cgcaccgtcc gtcctgccag cttatcatcc acgaagggca
     1021 gggcaatgcc cgcgaagttg ctgcctccgc cgtggcatcc gatgaccaca tccggctgct
     1081 cccctagctt ctcgagctgc tgcttggcct cgaggccaat gatggtctgg tgcaggagca
     1141 cgtggttgag caccgagccc agtgagtatt tgcagtcctc gtgcttcacg gtgtcctcga
     1201 gagcctcact gatggcgatg cccaggctcc ccggggtatc cgggtcatcg gcgagaacct
     1261 tccggccgat ctcggtcacc gtgctgggac tcgagtgaac cgcggcaccg aacacctgca
     1321 tgaaggaccg acggtacggc ttctggttgt agctaacgcg caccatgtag acggtgcact
     1381 cgagaccgaa catggcgcac gcgtgagcca acgcgctgcc ccactgcccc gctccggtct
     1441 ccgtggcgag acggcgaacg ccttcctgtt tgttcacgta ggcttgagcg atggcggtat
     1501 tcggcttgtg gcttcccgcg gcgctcacgc cctcgtactt gtagtagatg tgggcgggcg
     1561 tctcgagcgc ttcctcgagc cgaatcgcgc gcaccaacgg cgacggacgg taggtacgat
     1621 agatgtcgag cacgggcccc gggatgtcca cccaggggtc cgggcacatc tcctgcatga
     1681 ccaggccttc agggaagatg ggcaacagat ccgctgggcc ggcaggctgc cccgtggccg
     1741 ggttcagtgg aggggctagc ggagtcgaca acctgggcaa cacgttgtac caggcgcttg
     1801 gcatatcact agggggcaac aaatagcgaa tggacgcaga cacgtgaacg cctctcctta
     1861 tggtcgcatg gatatgcgcg cagggcgcgt gggctcggga gccctcagcc ggccgccaga
     1921 ccgcgcggca cacaatggtg tagtgtattc ccgcgggttc gcgtcacacc cttccacgtg
     1981 tgcgcatgca accaacccgc gtcgccccgg gcgcatctac tcacaaagag gccaagcgga
     2041 ggtgactcga tgcggcgcgc gacggtctgg gtcatagcgg tgggtgccct ctcgttcccc
     2101 gcctggcccc agtctcctga agtcctcgcc gaggtggagg ccgttattgg cccttggcgc
     2161 gtcgcggcgc cggccgacaa cgggttcgct gacttggtgg cggcgatcca gcgcgtcaca
     2221 gagggtgact ccgtcgatct cctcaatggc cccgacgccg ggccggcagc cgaggagctc
     2281 atcgccacga atgctgaggc cctggcggcg gttcgcgcgg ccatggggcg ggagtgtcgc
     2341 gtgccgcccg tcacgtcgtt cacgactccc ctcccgtacc tagccaatgc gcgtgtgacg
     2401 gcccgcgtac tgtgtgttga gggccgatgc cgggaggtcg acggcgaccc gtccggggct
     2461 cttggctcgt acctggacgc cgtccagctc ggcggcgcca tctcacgggg cggcaccctc
     2521 attcacgggc tggtggacgc agccgtcatc cggatcgcct ccgctgaagt gcggcggcta
     2581 tgcgaatctg gaccggcctt ccccgacgcg actctggccg acgccatcga ccggctgact
     2641 cggctcgagc gcgcaggtcc ttcgccccag cagttcctcg cgaccgagct ggaggcgagc
     2701 gtggcgggcc tgagagagat agcggagaac ccgcaactgc tcgagcagat cgccacggac
     2761 gcgggcatga gcaggtcgcg cggtaccatc gagctcgatg ccgaagcccg cgcgctcatt
     2821 gagtactatg ggcgccttat cgagctcctc gaccttccgt accgcgactc cctgggcgtc
     2881 gcgcagcctc ccgaggccgc agagacaccc ctggccgcca tggtagcgcc ctctgcagac
     2941 agggccagcg aacgtctccg cggcggcgcc gcagtgctcc gcggtgtgcg cgtcctcctg
     3001 gccatcgtgc ggttccgcca ggcacgagag gcctaccccg acaccctgag ccagcttgtt
     3061 cctgactact tgcccgccgc ggtagttgac ccctacacgc tcgagccctt cgtctacgca
     3121 ggcacaccga acgggagagc gctctactcg cccgggcccg atggggtgga cgacggcgcc
     3181 gatgaggaac gcgatgtggt gatcgccgcc ctgccggaat aggccctggc aaagccagtg
     3241 agccccggag gcagcccccg gggctccgtc tgatggccca gcgcgttccc agttatgccc
     3301 agtaggcct
//
LOCUS       DAOSPL010000371         3308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_28961_length_3308_cov_3.455272, whole genome shotgun sequence.
ACCESSION   DAOSPL010000371 DAOSPL010000000
VERSION     DAOSPL010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28961_length_3308_cov_3.455272"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PLQ54_07985"
     CDS             complement(<1..927)
                     /locus_tag="PLQ54_07985"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOS93237.1"
                     /translation="MNHQTHCSIALFALATAIGAEAEAPGDEALPLQMQAYLVREASR
                     ISSHALDGIHTAADWEAARAGVRRKLAFMMGLDLTAARSPLKAHVMGRLEREAFSVEK
                     VTFQSVPGLYVTGNLYVPKGLRGRAPAVLYVCGHGGKPPTGAKAQYQHHGIWLASHGY
                     VTLMIDTLQLGEVGGLHHGTHRENLWNWVSAGYAPGAVEVWNAVRALDYLQSRPEVDP
                     GTIGMTGRSGGGAYTWYTAALDDRVQVAIPVNSANTIYSHIEQDTLRGHCDCMFIHNC
                     YGLDFVDMDACIAPRPLLILESTDDGIFPRQGH"
     gene            complement(1201..2259)
                     /locus_tag="PLQ54_07990"
     CDS             complement(1201..2259)
                     /locus_tag="PLQ54_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS93238.1"
                     /translation="MAKKTLNVGIIGVGGIAGVHFPGWRESPLTQMYAFADLDGATLK
                     RVGEAQGVSVLYEDPMDLIADPNVDIVDVCTPNMYHCPLTVAALEAGKDVICEKPLAP
                     TPEDIKKMIAARDKSGRLLMTAQHFRFSGGAKALKTEVEAGMLGDIYHARSWMLRRSG
                     APTRPGFIEKKHSGGGPCIDIGVHILDLTLWMMGNPKPVSVSGVTKDILAHKPGAFSN
                     WGGPIPKSYDVEEFAAAFVRFETGATLVIEVSWLLNHNTPSEDMQMWLYGDKAGAHWP
                     SNEVYSTNNKTKQHFNTKLQREEGGAPHAKECMAFAEAVVKKLPSPVPAEQSLDVIAI
                     LDGLYRSAASGKEVVLKY"
     gene            2427..3164
                     /locus_tag="PLQ54_07995"
     CDS             2427..3164
                     /locus_tag="PLQ54_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOS93239.1"
                     /translation="MGIGRDFMNRTRYRFLEESEQQRGLPMPPLQWPVEPGAALIDLP
                     HPSGLEIAPVSVRELIEQRTSVRRYSAARLSLLELSYLLWCTQGVKEVGPGYLLRTVP
                     SAGAGHALEAYLIVNSVQGLEAGLYRFVAVGHRLARLKAPADLGERAVAACFGQSFVG
                     TSSATFVWVAIPHRMTWRYSERGYRYLHLDAGHACQNLYLAAEAIGGGVCAIGAFDDD
                     AMATLLDLDPDEQFVIYLATVGKKVVR"
BASE COUNT          502 a         1126 c         1090 g          590 t
ORIGIN      
        1 gtgcccctgg cgtgggaaga tcccgtcgtc agtcgattcg agtatgagca gcggccgagg
       61 cgcgatgcag gcgtccatgt cgacgaagtc cagcccatag cagttgtgga tgaacatgca
      121 gtcgcagtgg cccctgagcg tgtcctgctc gatgtggctg tagatcgtgt tggcgctgtt
      181 gacggggatg gcgacctgca cgcggtcgtc cagtgccgcg gtgtaccagg tatacgcgcc
      241 gccgccgctc cgcccggtca tgccgatcgt cccagggtcg acttcgggcc ggctctggag
      301 gtagtcgagg gcgcggacgg cgttccacac ctcgacggcc ccgggcgcat agccagccga
      361 cacccagttc cacaggttct cgcgatgtgt gccgtggtgc agcccgccga cctcacccaa
      421 ctgcaatgtg tcgatcatca gcgtgacgta gccgtgtgac gccagccaga tgccgtggtg
      481 ctgatactgc gccttggcgc ccgttggcgg cttgccgcca tggccgcaca catagagcac
      541 cgccggcgcg cggccgcgca gccccttcgg cacgtagagg ttgccggtca cgtagaggcc
      601 gggcacgctc tggaaggtca ccttctcgac cgagaatgcc tcgcgttcga ggcggcccat
      661 gacgtgcgcc ttcagcggcg accgggccgc ggtcagatcc aggcccatca tgaacgcgag
      721 cttccgccgg acgcccgccc gggccgcctc ccagtccgcg gccgtgtgaa tgccatccag
      781 cgcgtgcgag ctgatccggc tggcctctcg gacaaggtac gcctgcatct gcagcggcaa
      841 ggcctcgtcg cccggtgcct cggcctctgc cccgatagcg gtggccagtg cgaacagcgc
      901 gatggagcag tgggtctgat ggttcatggg cctcccccta acgcggcctt cccccccgtc
      961 gccttgcacc agcccggtcg gaggcccggc ttcggccggg cgcgcaccac caccgcgacg
     1021 gggtcttcgg gagcgcggac ttcagtccgc ccgggccctc tcgttggccc tcaatgggcc
     1081 agcgagaggg agccagcaga caccgtcacc gcgccagcca ccgctgcagc cccgagcttg
     1141 ccgaagagct gcgctcccga agccccgctc cggctgtggc ctcgcgcaat actgcgcacc
     1201 ctaatacttc agcacgacct ctttgcctga cgccgcgctc cggtacagcc cgtcaaggat
     1261 ggcaatgaca tccagcgact gctcggccgg caccggtgac gggagcttct tcacgaccgc
     1321 ctcggcgaat gccatgcact ccttggcgtg gggcgccccg ccttcctcgc gctgcagctt
     1381 ggtgttgaag tgctgcttgg tcttgttgtt ggtcgagtag acttcattgc tcggccagtg
     1441 ggctccggct ttgtccccgt agagccacat ctgcatgtct tcactcgggg tgttatggtt
     1501 gagcagccag ctcacctcga tgaccagcgt ggctccggtc tcgaagcgca cgaaggcggc
     1561 agcgaactcc tcgacatcat agctcttggg gataggaccg ccccagttgc tgaaagcgcc
     1621 aggcttgtgt gccagaatgt ccttcgtcac cccggagacg ctgacgggct tggggttgcc
     1681 catcatccag agggtcagat cgaggatgtg cacgccaata tcgatgcagg gtccgccccc
     1741 gctgtgcttc ttctcgatga acccgggccg cgtgggggcg ccgctgcggc ggagcatcca
     1801 gctccgggcg tggtagatgt cacccagcat accggcctct acctcggtct tgagcgcctt
     1861 cgcaccgccg ctgaaacgga agtgctgtgc ggtcatgagc agccggccgc tcttgtcgcg
     1921 ggccgcgatc atcttcttga tgtcctcagg agtgggtgcc agcggcttct cgcatatgac
     1981 gtccttgccg gcctcgagcg cggccacggt cagggggcag tggtacatgt tcggagtgca
     2041 gacgtcgacg atgtccacat tggggtcggc gatgaggtcc atgggatcct catagagcac
     2101 gctgaccccc tgcgcttcgc caactcgctt cagcgtcgcg ccatcgagat ccgcaaaggc
     2161 gtacatctgg gtcaggggac tctcgcgcca accggggaaa tggacgccgg caatgccgcc
     2221 gacgccgatg atgccgacat tcagggtctt cttggccatg ggtgtgtgtc ctcggtgtcg
     2281 atgggatagt tgcagcgcag ccttgcctgc gccacggtgc tcttgtgcgc tcgcaaccgc
     2341 cgcctttcct tcgccgccga cgagaaagac gggcaggatg cggccgggcg ggggagaaga
     2401 gcctcccatc acgagggggc actgccatgg gcatcggccg ggacttcatg aaccgcacgc
     2461 gctaccgctt cctcgaagag tccgagcagc agcgagggct gcccatgccc ccgctccagt
     2521 ggccggtcga gcccggcgcc gcgctgatcg atctacccca cccgtcgggc ctcgagatcg
     2581 cgcccgtcag cgtgcgggag ctgatcgagc agcgcaccag cgtgcggcgc tactcggcag
     2641 cgcgactcag cctcctcgag ctctcatacc tgctctggtg tactcaggga gtcaaggaag
     2701 tcgggccagg ctacctcttg cggacggtgc cctccgccgg agccgggcac gcactcgagg
     2761 cgtacctgat cgtcaactcg gtgcaggggc tcgaggccgg cctgtaccga ttcgtggccg
     2821 tggggcaccg gctggcacgc ctgaaggcac ccgccgacct gggcgagcgc gccgtggccg
     2881 cgtgcttcgg gcagagcttc gtcggcacat cgtcggcgac gttcgtgtgg gtggcgatcc
     2941 cgcaccggat gacctggcgg tactctgagc gcggctaccg ctacctgcac ctcgatgccg
     3001 gccacgcgtg ccagaacctc tacctggctg ccgaggccat cggcgggggc gtgtgcgcca
     3061 tcggcgcctt cgacgatgac gccatggcca cactcctcga cctcgacccc gacgagcagt
     3121 tcgtcatcta cctggcaacg gtagggaaga aggtggtacg ataacatagg aacgtgcatt
     3181 gccgcgggga ggccgtcgat ggcgcgaagg atgctctccc gaacccggtg tctgcggttc
     3241 ttcgttgctg ccgccgctct atgtgctgcg gctgcctggg gcgcgcccaa tgagcgtcct
     3301 cccgacct
//
LOCUS       DAOSPL010000372         3304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29009_length_3304_cov_2.790397, whole genome shotgun sequence.
ACCESSION   DAOSPL010000372 DAOSPL010000000
VERSION     DAOSPL010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29009_length_3304_cov_2.790397"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2529
                     /locus_tag="PLQ54_08000"
     CDS             <1..2529
                     /locus_tag="PLQ54_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93240.1"
                     /translation="LRERSRAFAAAQAHIAEATRAGGFHAAYAGAMAALGAPTNVIGD
                     TAFGTMIAWTRAAAECLARDVLHMGPATDPALGALIAAPLSGAEVGIQAYHALATALA
                     KAAERVEGDPTRLEQIAMTLELLDIALAIEKTQSADFGEELTLEIARFFDVTLNNHIF
                     DCIPYHYHAERGAGFQRLDRLEKIALAERRHRWLYTYLRGLLAADSDLAPLGAEYADA
                     WLGDADRGLLPLGVYGDGAEARWFSYARLRDASVLRHEGYPAPELLLDVDPAVLRCDQ
                     RANVVIVYPHGNTTVPVALEQGGRLAEEEGVNLFLCAWPAIVDAGPLPVLQLRDAFGY
                     ICPRDFERALIATGVDRAEAKARAKAAGTDGVLIAARFSRPVTAHGIFFHFTHPLRPR
                     IGSVRAPLIQPIIWEAATHLKCRLPEMLRGSGTRTAPQTNWLRADSESMTEAEAKARI
                     EAALRKMARSHDALIVKPEKESGGRNARMRPVREDGRVIDDHIIELRDLTYEISKTDN
                     VAIQEVIKSDVRRLYTREFLEGLVDRFARIGVPVLLDRDPPTPLFSYFRQVLVRGGED
                     YRISHHITVVSTRRIANVGQGGLLCEYTDDIIEPRYRRDLRNALTEAARGAMQAQRRY
                     LREHWREILDEYLAVHPEYFGSVPREIGEDLTGFSDCDIPYEMGDFMPVWLVDANDNL
                     VRAFDFDREELVPLFDRKGMPTDFRLFTIGGKPVRRFDGSGRPLSLPTSDPYGLPIPY
                     VDHRGRRVRTLVCYKIEANPGAGLWRPHNDQLPPERRGEGVRILFTALGQRAEAYKAA
                     LAERIPPTKPASRVPAGGRHVPASTPAAPAVREAVERAHHELRR"
     gene            2513..>3304
                     /locus_tag="PLQ54_08005"
     CDS             2513..>3304
                     /locus_tag="PLQ54_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93241.1"
                     /translation="MSFVADDLAVALQHYFERRPGLDVAIGSGRLEEFVARELAQPGL
                     LDQFVREMGLSKVGSGPVSHAEPGEGALTPFLAEYIAERARIGPDAPAMRIRPEPSGA
                     PQPIVAVVFTAEAVLIIDTVSALAEDAAPSGLAVVAVRDTWLDERGRALRAIVHDPGG
                     PADVRFSSPAAMAAAWILHLAPPEHETHRELSARLDRAGVRVLNPYQTSQRLDDKAET
                     HRLWRTAGVETPDWTLLPFGTSSVAAATMVADAVGRWGGAFILPND"
BASE COUNT          520 a         1193 c         1070 g          521 t
ORIGIN      
        1 ctgagggagc ggtcgcgcgc cttcgcggcg gcgcaagcgc acatcgctga agctacgcgg
       61 gccggcgggt tccacgccgc gtacgccggg gccatggcgg ccctgggggc acccacgaac
      121 gtcatcggcg acaccgcctt cggcacgatg attgcctgga cgcgggccgc agccgagtgc
      181 ctggcgcgcg atgtgctcca catgggcccc gccacggatc ccgcactcgg cgccctgatc
      241 gccgcccctc tctcgggcgc cgaggtgggc atccaggcct accacgccct cgccaccgcg
      301 ctggccaagg ccgccgagcg ggtcgagggc gacccgacca ggctcgaaca gatcgccatg
      361 accctcgagc ttctcgatat cgccttggcc atcgagaaga cccagtccgc ggatttcggc
      421 gaggagctca ctctcgagat cgcgcggttc ttcgatgtga ccctgaacaa tcacatcttc
      481 gactgcattc cctaccatta ccacgcagag cgcggggccg gcttccagcg cctcgatcgg
      541 ctggagaaga tcgccctggc cgagcgccgc catcgctggc tgtacaccta tctgcggggc
      601 ctcctggccg ccgatagcga cctggccccc ctcggggccg agtacgccga cgcgtggctg
      661 ggcgatgccg atcgggggct actgccgctc ggagtctacg gcgacggcgc cgaggcgcgg
      721 tggttctcct atgcccggct ccgtgacgcg tccgtcctcc gccacgaggg gtatcccgcg
      781 cccgagctcc tgctcgatgt cgatcccgct gtgcttcgct gcgaccagcg ggccaacgtc
      841 gtgatcgtgt acccccacgg caacaccacg gtgcccgtgg cactcgagca aggcgggcgg
      901 ctggcggaag aggaaggcgt caacctgttc ctctgcgcgt ggccggccat cgtcgacgcc
      961 ggcccactgc ccgtgctgca gctcagggat gcgtttgggt acatctgccc gcgagacttc
     1021 gaacgggcgc tgatcgccac cggagtcgac cgtgccgaag caaaggcacg agccaaggcg
     1081 gccggtaccg atggtgtgct cattgccgcg cggttctcgc ggcctgtcac ggctcatggc
     1141 atcttcttcc acttcaccca tccgctgcgt ccccgcatcg gcagcgtgcg cgcgccgctg
     1201 atccagccca tcatctggga ggcggccaca cacctcaagt gccggctgcc cgagatgctg
     1261 cgcggcagcg gcacccgcac agcgccgcag acgaactggc tcagggccga ctcggaatcc
     1321 atgaccgaag ccgaggccaa agccagaatc gaggcagcgc tccgcaagat ggcgaggtcg
     1381 cacgacgcgc tcatcgtcaa acccgagaag gagtcgggag ggcgcaatgc gcgcatgcgc
     1441 cccgtccgcg aggatggccg cgtgatcgat gaccacatca tcgagttgcg cgacctcacc
     1501 tacgagatct cgaagaccga caacgtcgca atacaggaag tcatcaagtc cgacgtccgc
     1561 cgcctgtaca cgcgcgagtt cctcgaaggc ctggtcgacc gcttcgcacg catcggagtg
     1621 cccgtgctgc tcgatcgcga ccccccgaca ccgctgttct cctacttccg tcaggtgctg
     1681 gtgcgcgggg gcgaggacta ccgcatctca caccacatca cggtggtcag cacccggcgc
     1741 atcgccaacg tcggccaagg ggggctgctg tgcgagtaca ccgacgacat catcgaaccg
     1801 cgctaccgtc gggacctgcg taacgccctg accgaggcgg ctcgcggagc catgcaggca
     1861 cagcgccgct atctgcgcga gcactggcgc gagatcctcg atgagtacct ggccgtccat
     1921 ccggagtact ttggcagcgt gccccgagag atcggcgagg acctgaccgg gttctccgac
     1981 tgcgacatcc cctatgagat gggcgatttc atgcccgtct ggttggtcga tgcgaacgac
     2041 aacctggtcc gcgccttcga cttcgatcgc gaggagctgg tcccgctgtt cgaccgcaag
     2101 ggcatgccca cggatttccg gctattcacg atcggtggca aaccagtgcg acgcttcgat
     2161 ggatccggcc ggccgctgtc cctgcccaca tccgatcctt acggtctgcc catcccctat
     2221 gtcgaccacc gcggtcgacg tgtccgcacg ttggtgtgct acaagatcga ggccaacccg
     2281 ggggcaggac tctggcggcc gcacaacgac cagctcccgc ccgagcgccg tggagagggc
     2341 gtccgcatac tgttcaccgc cctcgggcag agggccgagg cctacaaggc agccttggcc
     2401 gagcggatcc cgcccaccaa gccggcaagc cgggtgcctg cgggtgggcg ccatgtgccc
     2461 gcatcgactc ccgcagcccc tgcagttcgc gaggccgtgg agcgagcgca ccatgagctt
     2521 cgtcgctgac gatctcgccg ttgccctcca gcactatttc gagcgcaggc cgggactcga
     2581 cgtcgccatc ggctcgggtc gcctcgagga gttcgtggcc cgagagcttg cccagccagg
     2641 gcttctggac cagttcgtgc gggagatggg cctgtcgaaa gtgggatcgg gacccgtttc
     2701 ccacgcagag ccaggggaag gggccctgac gccgtttctg gccgagtaca tcgccgaacg
     2761 tgcccgcatc ggaccggacg cgcccgccat gcgcatccgg ccggagccct ccggcgcgcc
     2821 tcagcccatc gtcgctgtcg tcttcacggc cgaggccgtc ctgatcatcg acacggtctc
     2881 ggctctcgcc gaggatgccg caccctccgg gctcgccgtt gtggccgtgc gcgacacatg
     2941 gctcgacgag cgcggtcggg cgttgcgtgc tatcgtgcat gatcccggag gtccagcgga
     3001 cgtccgcttc tcgtcgcccg cggccatggc cgccgcctgg atcctgcacc tggctccgcc
     3061 ggagcacgag acacaccgcg aactgtccgc gcggctcgat cgggccggcg tgcgggtact
     3121 gaacccctac cagacgtccc agcgcctgga cgacaaggcc gagactcacc gcctgtggcg
     3181 gaccgccggg gttgagaccc ccgactggac gctgctgccc ttcggcacga gttcggtcgc
     3241 cgctgcgacc atggtcgccg atgccgtcgg ccgctggggc ggggcgttca tcctcccgaa
     3301 cgat
//
LOCUS       DAOSPL010000373         3303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29012_length_3303_cov_4.436884, whole genome shotgun sequence.
ACCESSION   DAOSPL010000373 DAOSPL010000000
VERSION     DAOSPL010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29012_length_3303_cov_4.436884"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..99)
                     /locus_tag="PLQ54_08010"
                     /pseudo
     CDS             complement(1..99)
                     /locus_tag="PLQ54_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009356261.1"
                     /note="catalyzes the formation of 5-phospho-alpha-D-ribose
                     1-phosphate from D-ribose 5-phosphate and ATP; incomplete;
                     partial in the middle of a contig; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
     gene            164..1318
                     /locus_tag="PLQ54_08015"
     CDS             164..1318
                     /locus_tag="PLQ54_08015"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOS93242.1"
                     /translation="MTAAPISGPRATRRFAYIDALRGFAALWVFGLHVHQHWLDDVRP
                     PILSADGILVRLIGFGGAGVDLFIVLSGFCLTLPLTRAGTAALSSINVRGFFRRRAFR
                     LLPAYYAALAAVIALELIPAIRPALVARDLTGLDVITHLTLTFPLSEATIGAVNGSLW
                     SIPLEATLYLGFPLLLWLAARRGISAVVLATLGIAAVWGLVTVSAIWPLPGGWLPAAD
                     KYLPPRWLGFAMGMYAATQVRGIPHPRAATHAVWACGLGLPAGLYGYAQGPDIVRTLG
                     FGALGAGLLVLLQRVPERWFGRGPLAWLVGLGTISYSFYLLHQPIILLLNGWGHSLGL
                     GPIPLFFVALATSGTLTFAVATALYRGVERPFLVRGSMSAVVIEGDRRAG"
     gene            1388..1489
                     /locus_tag="PLQ54_08020"
                     /pseudo
     CDS             1388..1489
                     /locus_tag="PLQ54_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020169766.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1577..2989
                     /locus_tag="PLQ54_08025"
     CDS             1577..2989
                     /locus_tag="PLQ54_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93243.1"
                     /translation="MIGQVLTAMLLGLGTCGMGAAFGRWVSRRIALPTSTNAAVSLLL
                     GIVGVGVLTLAIGHLGLLGPWVPWLIAGFGLIAGWPARKDALRFVQRAAAAIAAEART
                     RPLLAAGAAVAGLTALVATLAPPQRIDEVEYHWPAPLDWAASGGWTDSPFRHVDGFPL
                     MEIIYTAAATQGSYVAAHAMSLLTMVGIGLAAAGMVQSLGLTARLPVAAAAVLMPVVW
                     DGSYAAYNDTPGAAFTIAAAAVAVGIQRPSRAATALISALLAAAISIKPFHVVAVCCV
                     ALLLWHRFRRASLRSSATMAAVAAATVAFWSVRQWAYTGHLIDPLMFGGASGEAQARL
                     PSRIQHLLAPIFPFVSGVLGAQEPWGARTSAVLQVFLIPSLVYLVWSGRSAFGRFGVL
                     SGPAYLHWVVLGFVAVRTRFHIFTWVLLVPAVAYAVHHASKRWPRLGPWLDLAWTALV
                     LLGALDVSFEMWRAINTIRT"
     gene            3114..>3303
                     /locus_tag="PLQ54_08030"
     CDS             3114..>3303
                     /locus_tag="PLQ54_08030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016443149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS93244.1"
                     /translation="MKLIVQVPCLNEELTLAAVLTSIPTKIPGITSIEVVVIDDGCSD
                     RTVEIARAHGVQHIIRHAR"
BASE COUNT          464 a         1124 c         1062 g          653 t
ORIGIN      
        1 ggcgagcatc ggcaaggcca tgaggtggtc gaccggcccg tcgaagaagc cctgaatctg
       61 agcggtgtgc aggtcgaccg ccatgagccg accggccccg ccgtgcggaa caggccgccg
      121 ttcctcgatc gggacatggt ggaacgctga tatcgtccgt cgaatgacgg ccgccccgat
      181 atcgggccca cgagccacgc gtcgtttcgc ctacatcgat gctctccgcg gattcgccgc
      241 cctgtgggtt ttcggtctgc acgtccacca gcactggctc gatgacgtcc gcccgcccat
      301 tctcagcgcc gatgggatcc tcgtacgcct catcggcttc ggcggcgcag gcgtggatct
      361 gttcatcgtc ctgtccggct tctgtctcac cctgcctctc acgcgagcgg gcacggctgc
      421 gctctccagc atcaacgtcc gcggtttctt ccgccgccgg gccttccggc tgctgcccgc
      481 ctactacgcc gcgctcgccg cagttatcgc ccttgagttg atccccgcga tccggccggc
      541 gctggtcgcg agggacctca ccggccttga tgtcatcacc cacctcacgc tgaccttccc
      601 actgtcagag gccacgatcg gagccgtcaa cggctcgctc tggtcgattc ccctcgaagc
      661 caccctctac ctcgggttcc cgctgctgct ctggctcgcc gcccggcgag gcatcagcgc
      721 cgtggtcctg gccaccctgg gcatcgccgc ggtctggggg ctcgtcaccg tgtcggccat
      781 ctggccgctg cccggcggct ggttgccggc ggccgacaag tacctcccgc cccgttggct
      841 ggggttcgcg atggggatgt atgccgcgac gcaggtgcgc ggcatacctc acccgcgggc
      901 tgcgacccac gcggtctggg cgtgcgggct cggtcttccg gccggtctct acgggtatgc
      961 ccaggggccc gacatcgtgc gcacgctcgg tttcggggcg ctcggggcgg gcctgctcgt
     1021 cctgcttcag cgggtccccg agcggtggtt tgggcgcggc ccgctcgcct ggctggttgg
     1081 gctcgggacg atctcgtaca gtttctacct gctgcaccag ccgatcatcc tgttgctgaa
     1141 cggctggggc cacagcttgg ggctgggacc aatccccctg ttcttcgtcg cgctggccac
     1201 gtcgggcacg ctcacgttcg ccgtcgcgac ggcgctctac cgcggggtcg agcgaccctt
     1261 cctcgtgcgc gggagcatgt cagccgttgt catagagggc gaccgacgcg cggggtgact
     1321 ccgatggtga cgcgcgaccg tcgaagagga cgatgagctc gcgagcgctc gatgcccgcc
     1381 gtcggagatc cgtcagtcga agggccgggt cgggtcgtgt ttcgacaatg ccgccgccga
     1441 ggcgttcttc tccaccctgg aatgggaagt cctctctcgg cacgagttca ccgaccccga
     1501 ccatgcctgc aaagtggggt tgaccccgtt tcctctactc tgaggtggaa catgccaccg
     1561 ttgaggacgg agacaggtga taggccaggt gctcacggcc atgctccttg ggctcgggac
     1621 atgtggaatg ggggcggcat tcggccgttg ggtctcgcga cgcatcgccc tcccgacatc
     1681 aacgaacgcg gctgtatccc ttcttttggg catcgtaggg gtcggtgtcc tgacgctcgc
     1741 aatcgggcac ctcggcctgt tgggaccgtg ggttccttgg ctgatcgcgg gattcgggct
     1801 cattgctggg tggccagcgc gcaaggacgc ccttcggttt gttcagcgag ccgccgcggc
     1861 gatagcggcc gaagcccgaa cgcggccgtt gcttgccgcc ggagctgcag tggcggggct
     1921 cactgctttg gtcgcaaccc tggccccacc gcaacgaatc gacgaggtcg aataccactg
     1981 gccagcaccg cttgactggg cggcgtcagg cgggtggacc gacagcccct ttcgacacgt
     2041 tgacggcttc cccctgatgg agatcatcta caccgccgcg gcaacccaag ggtcctacgt
     2101 tgcggcgcac gccatgagtc tgttgacaat ggtcggcatt ggtttggccg ctgcgggcat
     2161 ggtgcagtcc ctcgggttga cagcgcgcct gcccgtggct gccgccgcag tcctcatgcc
     2221 cgtcgtgtgg gacgggtcat acgcggcata caacgacacg cccggcgcgg ccttcaccat
     2281 cgcggccgcc gcggtggccg tcggcataca gcggccaagt cgcgctgcaa cagcgctcat
     2341 ctcagcgctt ttggctgcag cgatcagcat caaaccgttt cacgtcgtcg cggtttgctg
     2401 tgtggctctc ctgctctggc atcgcttccg acgggcaagc ctacgatcgt cggcgaccat
     2461 ggccgccgtt gccgcagcga ccgttgcgtt ctggtcagtc cggcaatggg cttataccgg
     2521 tcatctcatc gacccattga tgttcggcgg agcatcaggc gaggcgcagg cgcgactccc
     2581 gtcacggatc caacacctcc tcgctccgat cttccctttc gtcagcggcg tgctaggcgc
     2641 gcaagagccc tggggtgccc gaacgtctgc cgtgttgcag gtgttcctca taccgagctt
     2701 ggtgtatctc gtatggagcg gtcggtcagc atttggtcga ttcggtgtgc tctctggacc
     2761 tgcctacctc cattgggtgg tcctgggctt cgtggcggtg cggactcggt tccacatctt
     2821 cacctgggtg ctccttgtcc ccgcagtggc gtatgccgtc catcacgcga gcaagaggtg
     2881 gccccggcta gggccatggc tcgacctggc gtggaccgcg ctggtgcttc ttggcgctct
     2941 tgatgtgtcc ttcgagatgt ggcgagccat caacaccatc agaacctgag ccggccagcg
     3001 aggggccacc ttcctcggag cctgtggagg caccgacagc acggcgttcg ggaccgattc
     3061 tcaagatact gagagcacgc ctgccgaggg cgtcatgatg cgatgatcat cgcgtgaagc
     3121 tcattgtcca ggtcccctgt ctgaacgagg agctgacact tgccgccgtc ctcacaagca
     3181 tcccgaccaa gatcccgggg atcacctcca tcgaggtcgt cgtcatcgat gacggttgca
     3241 gcgaccgaac cgtcgagatc gctcgtgcgc acggggtcca acacatcatc cgccatgccc
     3301 gca
//
LOCUS       DAOSPL010000374         3297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29097_length_3297_cov_3.093152, whole genome shotgun sequence.
ACCESSION   DAOSPL010000374 DAOSPL010000000
VERSION     DAOSPL010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29097_length_3297_cov_3.093152"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..2675
                     /locus_tag="PLQ54_08035"
     CDS             219..2675
                     /locus_tag="PLQ54_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93245.1"
                     /translation="MATDMLAVALIVTLWQAASSDRLPPNLLANPGFEDARADNPVPG
                     WQTRVSGDVYIRSESRPVAYGPDWPCDVASGAESVTIAARRGGGARLWQEVPAAAGAL
                     YSAAVWVKTYDADGQGFGHSPRDHASLRVDEISADGRIIHTHRTAGPRPATNRWVRLA
                     TSLQTDAATTRLRLTLKASIRFNHWHGRVKFDDASLCQVAALPSLQPEWRPPGGPEPV
                     SARHSEEPAPTIVVAERGGPPVSVVTSSSEPALAPVAEWCRSFLVQRGFSVLPCGQSA
                     SRHRNGPVWALEVARDSPVARSLDVRTGFLEHARGDAFALAAGRRDGRPTLGIVAKDV
                     AGVRAGMARLVAELWDEGSSFTQRSGRREYEPFIPVRLLSVAPTGRDAQGGPWADTLW
                     TNWTDERLRRYAEQIWLCGYNGIEVAEIRGYRAVYSDQELTRSITPKIRVLCSAARDY
                     GLKVRQFIWGQSLFVEGQNLCWNAPDERSRMLAEYRRLAQTYGDLVDELIVHVGDPGG
                     CDRNGCDAYKTTQEIAMALLAEYRRVNPKVTAVLSTWANAGFWRGHPNAQLLDETYSP
                     KELGIALHRWYDRDKVDCVRASGRAVDLWTWYLSDFEMAVDLTLAMRVVDRYYRALPA
                     SARGQIRGATADLCFHGWPQILGAYVAAQKMWDPQRGLSEIEHEFCAATFGERNADAM
                     VALYQACEAHVHPGRYAYFRPETDCPPVVLGTPDYDAQLRTALEQAGHVDLRLPHEPR
                     LVSATDPAALYQRLLDYGRLIGVASHAMVLLREARQTGAAANDLQAIVDAARTEAEPY
                     AADPGFGPLMDAVAAAARGT"
     gene            2693..>3297
                     /locus_tag="PLQ54_08040"
     CDS             2693..>3297
                     /locus_tag="PLQ54_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93246.1"
                     /translation="MRLCPLTALFVLALLTAARSSGAGNERALYNGIVLPDQWPPHPA
                     PLTDEPLPVPAYLATPPPVIPIDVGRQLFVDDFLIEETTLSRTYHAATYIEDNPVLTP
                     DKPWEFQGGHPCAMVFSDGVWFDPADRLYKMWYLGGYCQATCYATSEDGLHWSKPSLD
                     VVPGTNIVHNARRDSGTVWLDLEEKGPSRRFKMALYELDPPR"
BASE COUNT          554 a         1174 c         1063 g          506 t
ORIGIN      
        1 cgagcacaga accaatcccg ccacaactgc gcccagcgcg gccaggcaca gacacagtgg
       61 ctggctcagc cagttcggca aacccgagtc ccccttccgg acggcgggct ggtgcccgat
      121 ggcggacact ccggccttcg cccccccacc acgggcggcc tgcacaacgt ggcgccgcgt
      181 gaaggtgtcg actagaacga gctgcgaagc aggatgacat ggcaaccgac atgctcgccg
      241 tcgcgctgat cgtgaccctc tggcaggccg cgtcgtccga cagactgcca ccaaacctcc
      301 tggcgaaccc gggtttcgag gatgcccggg ccgacaaccc ggtacccgga tggcagaccc
      361 gcgtttccgg tgacgtctac atccgttccg agtcgcgccc cgtcgcctac ggacccgact
      421 ggccctgcga tgtggcctcc ggggcggagt ccgttaccat tgccgcgcgg agaggcggtg
      481 gtgcccggct gtggcaggag gtgcctgcgg cagccggcgc actgtactcc gcggccgtgt
      541 gggtgaagac gtacgacgca gacggccaag gcttcggcca ctcgccccgc gaccacgcct
      601 ccctgcgcgt cgacgagatc agcgccgacg gccgcatcat ccatacccac cggacggccg
      661 gaccccggcc cgccacgaac cgctgggtcc gattggccac ttcattgcaa acagacgccg
      721 cgaccacgcg tctgcggctc acactgaagg ccagcatccg cttcaaccac tggcatggcc
      781 gcgtgaagtt cgacgacgca tccctgtgcc aagtagcggc cctgccttcc ctgcagccgg
      841 agtggcgtcc ccccggcggg cccgaaccgg tgtcggccag gcattcggag gagccggcgc
      901 caaccatcgt cgtcgccgaa cggggtggcc cccccgtgtc ggtagtcacg tcgtcgagcg
      961 agccggcgct cgcacccgtt gcggagtggt gccgaagctt tctcgtccag cggggcttct
     1021 ctgtcctccc atgcgggcag agcgcctcac ggcaccgcaa cggccccgta tgggcgctgg
     1081 aggtcgcccg tgacagcccg gtggcccgat cactggatgt gcggaccggg ttcctcgaac
     1141 acgcccgcgg cgacgcgttc gcactggccg ccggccgccg tgatgggcgc ccgacgcttg
     1201 gcatcgtggc aaaagacgtt gccggagtgc gtgcgggcat ggcacgactg gtggccgagc
     1261 tgtgggacga ggggagttca ttcacacagc gctccgggcg gcgcgagtat gaacccttca
     1321 tcccagtccg cctgctctcg gtggcaccca cgggccgtga tgcccaaggc ggaccttggg
     1381 cggacacgct gtggacgaac tggaccgacg agcgcctccg acgctatgca gagcagattt
     1441 ggctctgcgg ctacaacggc atcgaggtcg ccgagatccg gggctaccga gccgtgtaca
     1501 gcgaccagga actgacccgg agcatcaccc cgaagatccg cgtcctgtgc tcagccgccc
     1561 gcgactacgg cctcaaggtc cgccagttca tctggggcca gagcctgttc gtcgaggggc
     1621 agaacctgtg ctggaacgcg cccgatgagc gatcccgaat gcttgcggag taccgccgcc
     1681 tggcccagac atacggcgat ctggttgatg agctcatcgt acacgtgggt gaccccggag
     1741 gctgcgaccg caacgggtgc gatgcctaca agaccactca ggagatcgcc atggcattgc
     1801 tggccgagta ccggcgggtg aacccgaagg tgaccgccgt actcagcacc tgggccaacg
     1861 ccgggttctg gagaggacat cccaacgccc aactactgga cgagacctac tcgcccaagg
     1921 agctgggcat cgcgctgcac cgctggtacg atcgcgacaa ggtcgattgc gtgcgggcct
     1981 cggggcgcgc cgtcgaccta tggacctggt acctgagtga cttcgagatg gccgttgacc
     2041 tgacgttggc catgcgcgtc gtcgaccgct actaccgggc gctgccggcc agcgcgcgtg
     2101 gccagatccg cggggcgacg gccgacctgt gcttccatgg ctggccacag atcctggggg
     2161 cctacgtagc cgctcagaag atgtgggacc cccagcgcgg tctgagcgag atcgagcacg
     2221 agttctgcgc ggccacattc ggtgagcgga atgccgacgc catggtggcg ctctatcagg
     2281 cgtgcgaggc ccatgtgcac cccgggcgct acgcctactt ccgtcccgaa accgactgcc
     2341 caccggtggt gctgggcacg cccgactacg acgcccagct ccgcacggcc ctcgagcagg
     2401 ccggccatgt cgaccttcgc ttgcctcacg aaccccggtt ggtctcggcc acggacccgg
     2461 ccgcgctgta ccagcgcctc ttggactacg gcaggctcat cggcgtggcc tcgcatgcaa
     2521 tggtccttct gcgcgaagct cggcagacag gtgccgcggc gaatgacctg caggccatcg
     2581 tcgacgcggc gcgtaccgag gccgagccgt acgccgccga tcccggcttt gggcccctga
     2641 tggacgcggt cgccgccgcg gcccgaggaa cgtgaggaag gacgtgtcac ccatgagact
     2701 ctgccctctg acggccctgt tcgtcctcgc acttctgact gcggcgcggt cgtcgggtgc
     2761 cggcaacgag agagcactgt acaacggcat cgtgcttccc gaccaatggc cgccccatcc
     2821 ggcacccctc acagacgaac cactgccggt gcctgcgtac ctggccaccc cccctcccgt
     2881 gatcccgatc gatgtgggcc gccagctctt cgtcgacgac ttcctcatcg aggagacaac
     2941 actctctcgc acctaccacg cggccaccta catcgaggac aatcccgtgt tgacgcccga
     3001 caagccatgg gagttccagg ggggccaccc gtgcgccatg gtgttcagcg atggcgtatg
     3061 gttcgacccg gccgaccggc tctacaagat gtggtacctg ggtggctact gccaagccac
     3121 ctgctacgcc acatcggagg atggcctgca ctggagcaaa ccgagcctgg atgtcgtgcc
     3181 cggcaccaac atcgtccaca acgcgagacg cgactcaggc accgtgtggc tcgacctgga
     3241 ggagaaaggc ccgtcgcgcc ggttcaagat ggccctctac gagctcgatc ccccccg
//
LOCUS       DAOSPL010000375         3294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29136_length_3294_cov_3.380673, whole genome shotgun sequence.
ACCESSION   DAOSPL010000375 DAOSPL010000000
VERSION     DAOSPL010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29136_length_3294_cov_3.380673"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="PLQ54_08045"
     CDS             complement(<1..1054)
                     /locus_tag="PLQ54_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS93247.1"
                     /translation="MRNTPVIKWLDHADLAVRLTPTVGRAEYLDHMTFVRNDRPLFTE
                     PFGPLIGLKEEWEAQGASPGELDLSAFRFRCPAEAYVHVNTGWMGETEEEVIEETDQL
                     RIYRDSMGRTMKLIKGFATLALPLDFPVKTMDDWRRIRHHYEFSEQRFAAGWEAAARE
                     HLARDRVLCAAIPGGYDTPRQLMGDAAACVAYHDDPELMHDILDTLTETAVRVLDRVS
                     RTVAIDQLSVHEDMAGKSGPLAGPSQVREFIAPYYRRVWDMLADRGTRLFDQDSDGNM
                     NAVIDEFLDAGINLMHPIEPAAGMDIVALRERYGRRLAFCGGLDKHVLRRSREEIDAE
                     LERKIPPLVATGGCMLG"
     gene            complement(1139..1819)
                     /locus_tag="PLQ54_08050"
     CDS             complement(1139..1819)
                     /locus_tag="PLQ54_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOS93248.1"
                     /translation="MRRSRGFTLIELLVVIAIIAILAAILFPVFAKAREKARQASCSS
                     NLKQLGLAWMMYATDESERIPPWLLLAGTSTAHCSTANTLWAHHVLQPYVKNWQLNIC
                     PSTGYTHSAGCSFIIPPVRDYGTSYSYNCKAIGCWCTSKSLGQFARPAELALMADGGF
                     GCMRPFFGQNGACGTTYIEPHNGGVNICFFDGHVKWMKSSKFYAPNSTVFSNYLPWAN
                     AESYPHGW"
     gene            complement(1918..2145)
                     /locus_tag="PLQ54_08055"
     CDS             complement(1918..2145)
                     /locus_tag="PLQ54_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93249.1"
                     /translation="MLSCLLLVALGCARTEQGTKAIPKQISPDEFKASIDKTKMMGGG
                     DASKTRGASGPAAPSAPTSSDAGQGTGQTGE"
     gene            complement(2660..3292)
                     /locus_tag="PLQ54_08060"
     CDS             complement(2660..3292)
                     /locus_tag="PLQ54_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOS93250.1"
                     /translation="MAIIAILAAILFPVFAKAREKARQTSCLSNLKQLGLAWNMYVTD
                     QSESVPPWLLRTGPSAGCNGSGQTLWVHHVLQPYMKNWEMNVCPSTGYKHGAGCGFIH
                     PSASPYGISYAYNCRAIGCWCNDKRLGQFSRVSELALFADGAWGCMRPWMRGGGCGTD
                     YIEPHNGGVNVAFFDGHTKWMKSSRFWAPNQATFNAYLPWSNAESYMPGW"
BASE COUNT          576 a         1064 c         1059 g          595 t
ORIGIN      
        1 gaccaagcat gcacccgccg gtggccacca gaggaggtat cttccgttcg agctcggcgt
       61 cgatctcctc gcgtgatcgg cgcagcacat gcttgtcgag cccaccacag aaggcgagac
      121 gccgaccgta gcgttcccgc agtgcgacaa tgtccatccc cgctgccggc tcaatggggt
      181 gcatcaggtt gatacccgcg tccaggaact cgtcgatcac cgcattcatg ttgccgtccg
      241 aatcctgatc gaacagccga gttccgcggt cggcgagcat gtcccacacg cggcggtagt
      301 aaggggcaat gaactcgcgc acctggctgg gtcccgccag gggtccgctc ttcccggcca
      361 tatcttcgtg gaccgagagc tggtcgatgg cgacggtccg cgagacgcgg tcaagcacgc
      421 gaacggcggt ctccgtcagc gtgtcgagta tgtcgtgcat gagctcgggg tcgtcgtggt
      481 aagccacaca tgcggcggca tcacccatca gctggcgggg ggtatcgtag ccgccaggga
      541 tggcggcaca caggacgcgg tcgcgggcca ggtgttctcg tgctgcggcc tcccagccgg
      601 ccgcaaagcg ctgctctgag aactcgtagt ggtggcgaat ccgcctccag tcatccatgg
      661 tcttcacggg aaagtcgagc gggagggcca gggtggcgaa gcccttgatc agcttcatgg
      721 tccgccccat gctgtcgcgg tagatgcgca actgatctgt ttcctcaatg acctcctctt
      781 cggtctcgcc catccaaccc gtgttgacat gcacatacgc ctcagccgga caacggaagc
      841 gaaaagcaga caagtcgagt tcgccgggtg acgcgccctg ggcctcccac tcctccttga
      901 ggccgatgag tggcccaaag ggctccgtga acagtgggcg atcgttgcgg acgaacgtca
      961 tgtgatccag atactcggcc cgccccacgg tgggggtcag gcgtaccgcc aggtcggcgt
     1021 gatcgagcca cttgatgacg ggcgtgttac gcacacctcc catgtaccag cggggccggg
     1081 aaccggccgg gccccgcaag tggtgcgggg cccggccatg tcactcgcag gtcatcacct
     1141 accacccgtg cgggtaggac tcggcgttcg cccaagggag gtagttgctg aagacggtgg
     1201 agttcggcgc gtagaacttg ctgctcttca tccacttcac atggccgtca aagaagcaga
     1261 tgttgacgcc accattgtgg ggctcgatgt aggtcgtccc acaggcaccg ttctgaccga
     1321 agaacgggcg catgcacccg aagccaccgt cagccatcag cgccagttcg gccggccgag
     1381 cgaactggcc cagcgatttg ctggtgcacc agcagccgat cgctttgcag ttgtaggagt
     1441 agctggtgcc gtaatcacga acgggcggga tgatgaagct gcaccccgcg gagtgagtgt
     1501 agccagtgct cgggcagatg ttcagctgcc agttcttcac atagggctga agcacatggt
     1561 gtgcccatag ggtgttcgcc gtgctgcagt gggcggtgct ggtccctgcc agcagcagcc
     1621 agggaggaat ccgttcgctc tcgtccgtgg cgtacatcat ccaggccaga cccagctgct
     1681 tgaggttgct cgaacacgat gcttggcgcg ctttctcacg agctttcgca aacacgggga
     1741 acaggatcgc cgccaggatg gcgatgatcg cgatgaccac caggagttcg atgagcgtga
     1801 agcctcttga cctacgcacc gcatatcctc cttcgctccg ccgccgaggg ccgtcgcgca
     1861 gttggcttgt ggtgctcgga ccggccctgg cctgatccac gtctcaggtt gggtgctcta
     1921 ctcgcccgtc tgtcccgtac cctgtcccgc atcggaactc gtgggtgccg agggggcagc
     1981 cggacctgac gcgccgcgtg tcttgctcgc gtcgcctccg cccatcatct tggtcttgtc
     2041 gatgctcgcc ttgaactcgt cggggctgat ctgtttgggg atggccttgg tcccctgctc
     2101 tgtccgggcg catcccagag ctaccaggag aagacatgag agcatacata tcgcgacact
     2161 gaccgaaaca cacctagcga gaagcgaacc cctctccaca gcagacctcc ttacgcggat
     2221 ggcgcggctg cacccgcagg gtatagccac gcagacgtcc ccaaccgcac caaatgccgc
     2281 ccctgccacg cccgaggtgc cccttcgcgg tgccggagag caaccgtgca actacttccc
     2341 gccgcgtcag gggaaacctc tacgtgccgc caagatccct gcggtcttgc agagaggcgg
     2401 tattggaggt cgactgggct gcagcgtggg gccgagcctc tgtgtcgacg cgtcccccgg
     2461 gcccgggcgc atccggcgcc tcggtgccgc tggagactgg gggctaagct ctgcgcgagc
     2521 gtgcgagcag tgccgacgcc cgcgggctcg ccaggcaagg aggggcggca gggtcacctc
     2581 catcgagtcg tggtggtctt tgggcctccg ggcgcgggcc gcccgcgtcc ggaggccccg
     2641 agcagttgcc gggcgtggcc taccagcctg gcatgtaact ctcagcgtta gaccagggca
     2701 ggtaggcgtt gaatgtcgcc tggttcggtg cccagaagcg gctgctcttc atccacttcg
     2761 tgtggccatc gaagaacgcg acattgacgc cgccgttgtg cggctcgatg tagtcggtcc
     2821 cgcagccgcc gccacgcatc caggggcgca tgcaccccca cgcgccgtcc gcgaacagcg
     2881 ccagctccga aacccggctg aactgaccca gccgcttgtc gttacaccag cacccgatgg
     2941 cacggcagtt gtaggcatag ctgatgccat agggactggc gctgggatgg atgaagccac
     3001 agcccgctcc gtgcttgtag cccgtcgagg ggcacacgtt catctcccag ttcttcatat
     3061 acggctgaag gacgtgatgc acccacagag tctggccact tccgttgcat ccggccgagg
     3121 ggccggtccg aaggagccag ggcgggactg actcgctctg atcggtcacg tacatgttcc
     3181 aagccagacc gagctgcttc aggttgctca gacaggaggt ctggcgtgcc ttctcgcgag
     3241 ccttggcgaa cacggggaac aggatcgccg ccagaatggc gatgatcgcg atga
//
LOCUS       DAOSPL010000376         3280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29293_length_3280_cov_3.391628, whole genome shotgun sequence.
ACCESSION   DAOSPL010000376 DAOSPL010000000
VERSION     DAOSPL010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29293_length_3280_cov_3.391628"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PLQ54_08065"
     CDS             <1..559
                     /locus_tag="PLQ54_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93251.1"
                     /translation="IRTGKFEPWVEIASRDKTCGWISPCDLYVAPDGAVHVLWTERAI
                     DERLRDRFFPEAKQRHALEYAIVRDGQVTHRRTLLEAGEGLGGEVPGQHRFQVTPDGR
                     LFVFGYVSGVNAAGEGLSEDRLLELRDGAPAGPAVRVPLEYPFSSFFTATVRAGSPPS
                     YTLDLLGECPGESHTIRYARVRLAS"
     gene            complement(665..1585)
                     /locus_tag="PLQ54_08070"
     CDS             complement(665..1585)
                     /locus_tag="PLQ54_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93252.1"
                     /translation="MRVSRLIWTAAAVGLALGVPGTSAQAPGDSAPERVLFAEPFEDA
                     GWAARGWYDGPHMELTADEHAPGSVHSCAWHWAEAGAVSPRGGGARVRLEPVDNVTLS
                     FWIKHSANWAWTGLGWHPHEFHFTTTEDDEYVGPAYTHLTFYVEAVDGKPRVAIQDGR
                     NIDQDRIGQDLVGITEHRAVAGGNGDSDGTGAGDHYRNGDVYWNGKSWDADGIYFGDE
                     AGPRYKGDWHHVKVRLKLNSVVDGVGQKDGVLQYWFDGELLMDHSRLVFRTGQHPNMR
                     INQFLMAPYYGPGVPHAQTIWVDDLTITTQ"
     gene            1807..2799
                     /locus_tag="PLQ54_08075"
     CDS             1807..2799
                     /locus_tag="PLQ54_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOS93253.1"
                     /translation="MGDQSGELPTTRVRCAPVECLRHSTARCICEVTTALLPSYVQDG
                     SDVPMGASGRIPSLQHVVQALHCLQDVLFPGRMSSELTEQTSLEAFVEERLSQAYRLL
                     LVEVEKALPFRWTGALAASEGKTEPMSDARAEANRITAEFLTRIPAIRAMLVKDVEAA
                     YRGDPAATTYAEIMVSYPGLKAITTHRIAHELYRLQVPLIPRIMSEHAHSHTGIDIHP
                     GAEIGESFFIDHGTGVVIGETCEIGRNVKLYQGVTLGARSFKVADDGTLVKGIKRHPT
                     LEDDVVVYANATILGGDTVIGKGAVIGSSVWILSSVPPGATVVIRDANTEVRTP"
     gene            2814..>3280
                     /locus_tag="PLQ54_08080"
     CDS             2814..>3280
                     /locus_tag="PLQ54_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93254.1"
                     /translation="MPSSLRQGGLIWSVAVFLLTWVPTSHCLAQADAKVRAIVVSPKP
                     LVIYINQRQSMSARIEFDGAADPNLRPAWTVAGPIGSIDADGTFTAGPRRAHGTLSAA
                     AGGQTDTVDVHVVPYAYGACHADGRIVYSVQDGGDSEYDVMLMDADGSNPRNLT"
BASE COUNT          549 a         1047 c         1115 g          569 t
ORIGIN      
        1 cattcgcacg ggcaagttcg agccgtgggt ggagatcgcc agccgcgaca agacctgcgg
       61 atggatctcg ccctgcgacc tctacgtggc tccagacggc gccgtccatg tcctgtggac
      121 cgaacgggcc atcgacgagc ggctccgcga ccgcttcttc cccgaggcga agcagcgaca
      181 cgcactggag tacgccatcg tccgcgatgg ccaggtgacg caccggcgaa ccctgctcga
      241 agccggcgag ggtctgggcg gtgaggtgcc cgggcagcat cgattccagg tcacccccga
      301 tggccgcctc ttcgtgttcg gctatgtcag cggagtgaac gcggcgggcg aggggctctc
      361 cgaggatcgg ctcctcgagc tgcgggacgg tgctccggcg gggccagccg ttcgagtgcc
      421 cctagagtat cctttttctt cattcttcac agccactgtc cgggccggtt cgcccccctc
      481 gtacaccctg gatctgctcg gcgagtgccc cggagagagc cataccattc gctatgctcg
      541 cgtacgtctc gcgagctaga ttcgggcccc gaatggtaag gaaatggacg agattcctgt
      601 tctcggaggc atgcgactgg tcatatgagc atgtgagtat accaggcgcg cccgtgacga
      661 cggcctactg ggtggtgatc gtgagatcat cgacccagat ggtctgagcg tgcggcacac
      721 ccgggccata gtacggtgcc atgaggaact ggttgatcct catgttcggg tgctgccccg
      781 tgcggaaaac gagccggcta tggtccatca gcagctcgcc gtcgaaccag tactgcaaca
      841 ccccatcctt ctgaccgacg ccgtccacca ctgagttcag cttcagcctg accttcacat
      901 ggtgccagtc gcccttgtag cgggggccag cttcgtcgcc gaagtagatg ccatcggcat
      961 cccagctctt cccgttccaa tacacgtcgc cgttccggta gtggtccccg gccccggtgc
     1021 cgtcgctgtc cccgttgccg ccggccacgg cgcgatgctc tgtgatgccg accaggtcct
     1081 gcccgatgcg gtcctgatcg atgttgcggc catcctgaat ggcgacccgc ggcttgccat
     1141 cgacggcttc gacgtagaag gtgagatgag tataggcggg accgacgtac tcatcatctt
     1201 ctgtggtggt aaaatgaaac tcgtgtggat gccacccgag cccggtccag gcccagtttg
     1261 cgctgtgctt gatccagaag ctcagcgtga cgttgtcgac gggttccagg cggacccggg
     1321 cgccaccgcc ccgcggagac acggcgccgg cctccgccca gtgccacgcg caggagtgga
     1381 cgctgccagg cgcgtgctcg tccgccgtga gctccatgtg ggggccgtcg taccagcccc
     1441 tcgctgccca gccggcgtcc tcgaagggtt cggcgaagag cactcgctcg ggggccgaat
     1501 cgccgggagc ctgggccgag gtgccgggca cgcccagggc gaggccgacg gcggccgcgg
     1561 tccagatgag ccgagacacg cgcatgggac gcacctccgt ggactggttt cgccgaaagg
     1621 ccatcgccgt cctgccccgt agccgcccct gttgtgtgtc gaggcgtcac gtggctacaa
     1681 tcggcttccg ccaatcgcct gggcggagga actcctgcct ccggggccgc gttggtggcc
     1741 gaaagcggcc ctcgcggctg gccgagcagc cggtggtaat ctggcgggac gaaggcggta
     1801 ggatcgatgg gagatcaatc cggtgagttg ccgaccactc gagtgcgttg cgcgcccgtg
     1861 gagtgcctga ggcacagcac cgcgcggtgc atctgtgagg ttaccacggc gctgctgccg
     1921 agctacgtgc aggacggctc cgacgtgccc atgggcgcct cgggccgcat cccgtcgctc
     1981 cagcacgtgg tgcaggcgct gcactgcctg caagacgtgc tgttccccgg gcgaatgtcg
     2041 tccgagctga cggagcagac cagtctggag gctttcgtcg aggagcggct gagccaggcg
     2101 taccggctcc tgctggtcga ggtggagaag gcgttaccgt tccgctggac cggggccctg
     2161 gcggcatcgg aggggaagac cgaaccgatg tctgacgcgc gggccgaggc gaatcgcatc
     2221 accgcggagt tcctgacgcg cattccggcg atccgcgcta tgctggtgaa agatgtggag
     2281 gccgcatatc gaggcgatcc ggcggcgacc acctatgcgg agatcatggt gagctatccg
     2341 ggactgaagg ccatcacgac acaccgcatt gctcacgagc tctaccggct tcaggttccg
     2401 ctgattcccc ggatcatgag cgagcatgcc cactcgcaca cgggaattga catccacccg
     2461 ggcgccgaga ttggcgagag cttcttcatc gaccacggaa cgggtgtggt cattggcgag
     2521 acgtgcgaga tcggacggaa tgtgaagctg taccagggag tgacactggg cgcgcggagt
     2581 ttcaaggttg ctgacgacgg gacgctggtc aaggggatca agcgccatcc gacgctcgag
     2641 gatgacgtgg tggtgtacgc caacgcgacc atcctgggcg gcgacacggt gatcggtaag
     2701 ggcgcggtga tcggcagcag cgtgtggatc ctcagctcgg taccaccggg cgcaaccgtg
     2761 gtgatccgcg atgcgaacac cgaggtgcgt accccatagg aatgaggttc accatgccaa
     2821 gttcgctccg ccaaggcggt ctgatctggt cggtcgcagt tttcctgctg acttgggtcc
     2881 ccacgtccca ctgcctggct caggccgacg cgaaggtccg ggcgatcgtg gtctcgccga
     2941 agccattggt gatctacatc aaccagaggc agtcgatgag cgcccggatc gagttcgacg
     3001 gcgcggccga cccgaacttg aggcccgcct ggacggtcgc gggcccgatc ggctccatcg
     3061 atgccgacgg gaccttcacg gcggggcccc ggcgcgccca cgggaccctg tcggcggctg
     3121 cgggcgggca gacggacacg gtcgacgtgc atgtggtccc ctatgcatac ggggcgtgcc
     3181 acgccgacgg acggatcgtc tactcggtgc aagacggcgg tgatagcgag tacgacgtga
     3241 tgctgatgga cgccgatggc tcgaacccga ggaacctgac
//
LOCUS       DAOSPL010000377         3278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29321_length_3278_cov_2.851381, whole genome shotgun sequence.
ACCESSION   DAOSPL010000377 DAOSPL010000000
VERSION     DAOSPL010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29321_length_3278_cov_2.851381"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="PLQ54_08085"
     CDS             <1..1150
                     /locus_tag="PLQ54_08085"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOS93255.1"
                     /translation="KVQGFERIARETFGRKYAIGVTSGTAALHAAFVAAGVGPGTEVI
                     VPAIGFFATAAAVVHSKAVPVFCDVDESLAMDPAKIESRITPRTVALAPTHVMGSVCD
                     MESIMAVARKHNLRVIEDCAQSCGGRQKGRYVGTFGDLGCFSISAYKIVGGGEGGLII
                     TDDERLYERAQGMVECGGLWRPDRFAPPRYPRELFCGTNYRMSELEAAIDQVQLRRMP
                     GTVARFHKVKTSILRQLRTFREITPQKLNDPDGEIGYTLRFFPETFELGAQIVEALNA
                     EGIGCGMRGPNGAPDWHVYHDMFPVTTKTGATEDDCPYQCPLYLQRGGNISYQRGDCP
                     VADDLFDRCISVGLNQWYTENDCNNIAAGINKVLSAFCTEDPNATRWM"
     gene            1312..2475
                     /locus_tag="PLQ54_08090"
     CDS             1312..2475
                     /locus_tag="PLQ54_08090"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOS93256.1"
                     /translation="MYRVGQKEIDAVARVLLSGELFRYHKGGECEKFEKRYARKLGVK
                     HVRMTSSGTTALTAALAGLQIGPGDEVLVPACTYMATAVSVLAVGAIPVVVDVDASIT
                     ISPKAIEAAIGPRTRAVIPVHMWGLACDMNRIMRIAKKHDLLVIEDACQGVGGAYEGR
                     MLGSIGHAGAFSFNYFKNMTCGEGGAVVTNDDEVIRRAGCMIDCCSFYWSGREEDFVP
                     FTASGSRATEIEGAIMNAQLDRIDGMIRAMRAQKKRIIEETADSGLKPVPAHSLDWEC
                     GSHVIYQLPTEAKADQFAALVGGTVCGKTGRHVYTEWDPLFHHQGAHHPALNPFALPQ
                     NKDCRMVYSKDMCSASLGFLNRSVMIGNHPDRTAGQVKALIRRINQAAAQVLA"
     gene            2540..>3278
                     /locus_tag="PLQ54_08095"
     CDS             2540..>3278
                     /locus_tag="PLQ54_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93257.1"
                     /translation="MGAASLGGMAWMGGGATRACVPPSFCVPTVLYPGCPIEHILEQI
                     SRTGATGVDVWDTGSAGPANPMQWMATHGPEALARRAGRFGLRIRAFSLFWTPGADLA
                     GRLEWLRDAGGRLVVLNGGGAPGRPPWEALRPLEWVVEAAAARDLEVAIVNHRGTSLC
                     SVQSIRQFTGACGWPRLGLALAPHHLAGGDTIEDAIAAAGDRLRLRYVVDGAGDALDR
                     MHPDTEICLFTRERMPARQMTRALPWAR"
BASE COUNT          522 a         1089 c         1106 g          561 t
ORIGIN      
        1 caaggtgcag ggctttgagc gcatcgcccg tgagaccttc ggccgcaagt acgccatcgg
       61 tgtgacgtcg ggcacggcgg cgctgcacgc ggctttcgtg gcggcgggcg tcgggccggg
      121 caccgaggtg atcgtccccg ccatcgggtt cttcgccacc gcggccgccg tggtccactc
      181 caaggccgtg ccggtcttct gcgatgtcga cgagtccctg gccatggatc ctgccaagat
      241 cgagtcgagg atcacccctc gcaccgtcgc cttggcgccg acgcacgtca tgggcagcgt
      301 gtgtgacatg gagtccatta tggccgtggc gcgcaagcac aatctgcgcg tcatcgagga
      361 ttgcgcccag tcctgtggtg ggaggcagaa ggggcgctac gttgggacct tcggcgatct
      421 gggctgcttc agcatctcag cctacaagat cgtcggaggt ggcgagggtg gcctgatcat
      481 caccgacgac gagcggctct acgagcgcgc ccagggcatg gtcgagtgcg gaggtctgtg
      541 gcgccccgac cgtttcgctc ccccgcgcta cccgcgcgag ctcttctgcg gcaccaacta
      601 ccgcatgtcg gagctcgaag cggctatcga ccaggttcag ctcaggcgca tgcccggtac
      661 ggtggcccgc ttccacaagg tgaagacgag catactcagg caacttcgca ccttccgcga
      721 gatcacgccc cagaagctca atgatcccga cggcgagatc gggtacacac ttcgcttctt
      781 ccctgagacg ttcgagctcg gggcccagat cgtcgaggcc ctgaacgccg aaggcatcgg
      841 gtgcggcatg cgtggcccca acggcgcgcc cgactggcac gtctatcacg acatgttccc
      901 ggtcacgacg aagaccggtg ctaccgagga tgactgcccc taccagtgcc ccttgtacct
      961 gcagcgaggc ggcaacatct cgtaccagag gggcgactgc ccggtggccg acgacctgtt
     1021 cgaccgctgc atcagtgtcg ggctcaacca gtggtacacc gagaacgact gcaacaacat
     1081 cgctgccggc atcaacaagg tcctctcggc attctgcacc gaggacccca acgcgacgag
     1141 gtggatgtag gtggctgtcg ttcgtgtcgg cgggccccct ggtccgcgct tgatgtcgtc
     1201 gtgcccgcgg gtctcctggc ccgcgccatt gacctaggcc ccgcgatggc gggcgagtgg
     1261 cgggcgagga cgcccgccgg cacgggacag ggttagctac ggaggcgaga catgtaccgc
     1321 gtgggccaga aggagatcga cgccgtcgcc agggtgctgc tgagcggcga gctgttccgc
     1381 taccacaagg gtggcgagtg cgagaagttc gagaagcgct atgcccgcaa gctcggcgtg
     1441 aagcacgtcc gcatgacctc cagcggtacc acggccctca cggcggccct ggccggtctt
     1501 cagatcggcc cgggcgatga ggtgctcgtg cccgcatgca cctatatggc gacggctgtc
     1561 tcggtcctcg ccgtgggggc cattccggtc gtggtcgacg tcgacgcctc tatcaccatc
     1621 agtcccaagg ccatcgaagc ggcgatcggc ccgcggacgc gtgccgtgat ccccgtgcac
     1681 atgtggggtc tcgcgtgcga catgaaccgc atcatgcgca tcgccaagaa gcatgacctg
     1741 ctggtcatcg aggatgcctg ccagggcgtc ggcggcgcct atgaaggccg catgctcggt
     1801 tctatcggcc atgcgggggc atttagcttt aactatttca agaacatgac atgtggcgag
     1861 ggcggtgccg tggtcaccaa cgatgacgag gtcatccgcc gcgccgggtg tatgatcgac
     1921 tgctgcagct tctactggtc gggccgcgaa gaggactttg tccccttcac cgccagtggc
     1981 tcccgagcca ccgagatcga gggggccatc atgaacgccc agctcgaccg catcgatggc
     2041 atgatccggg ccatgcgcgc gcagaagaag cgcatcatcg aggagaccgc cgactcgggc
     2101 ctgaagcccg ttccggccca cagtctcgac tgggagtgcg gctcccacgt gatctaccag
     2161 ctcccgaccg aggccaaggc cgaccagttc gccgccctgg tgggcggcac cgtgtgcggc
     2221 aagaccggcc ggcatgtgta caccgaatgg gatccgctct tccatcacca gggggcgcac
     2281 caccctgccc tgaacccgtt cgcgctgcct cagaacaagg actgccgcat ggtctacagc
     2341 aaggacatgt gcagcgcgtc cctgggattc ctgaaccgca gtgtcatgat tggcaaccat
     2401 ccggaccgta ccgcgggcca ggtgaaggcc ctcatccgca ggatcaacca ggccgcggca
     2461 caggtgctcg cgtagccctc gggcgacggc cgacggaggc aggcgtggga ggttgctgac
     2521 gcggcgaggg tttctgcgca tgggtgcggc ctctctgggg ggcatggcat ggatgggcgg
     2581 cggcgcgacg cgcgcttgtg tgccgccgtc gttctgcgtc ccgaccgtgc tctacccggg
     2641 ttgcccgatc gagcacatcc tcgagcagat ctcgcgcacg ggtgccaccg gtgttgatgt
     2701 ctgggacacc ggctctgccg gcccggcgaa ccccatgcag tggatggcca cgcacgggcc
     2761 cgaggcgctg gcccgtcgag cgggccgctt tggcctccgc atccgcgcct tctctttgtt
     2821 ctggacgcca ggagcggatc tggcaggtcg tctggagtgg ttgcgggacg cggggggccg
     2881 gctcgtcgtc ctcaatggtg gcggcgcgcc gggtcgcccg ccgtgggagg cgttgcgccc
     2941 tctcgaatgg gtggttgagg ccgccgctgc tcgagatctc gaggttgcca tcgtgaacca
     3001 ccgtggcacg tccctctgtt cggtgcagtc catccgccag ttcactggcg catgtggctg
     3061 gccccggctt ggcctcgctc tggcacccca ccatctggcg ggcggagaca ccatcgagga
     3121 cgccatagcc gccgcgggcg atcgactccg gctgcgctac gtggtcgacg gggcgggaga
     3181 tgccctggac cggatgcatc ccgacaccga gatctgcttg ttcactcgcg aacgcatgcc
     3241 cgctcgccag atgactcgcg ccctgccgtg ggcgcgcg
//
LOCUS       DAOSPL010000378         3277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29327_length_3277_cov_3.758535, whole genome shotgun sequence.
ACCESSION   DAOSPL010000378 DAOSPL010000000
VERSION     DAOSPL010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29327_length_3277_cov_3.758535"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="PLQ54_08100"
     CDS             complement(<1..924)
                     /locus_tag="PLQ54_08100"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOS93258.1"
                     /translation="MTPRQGAALLRELQPEALLDSIGEGAYLVDVDRTIVFWNQSAER
                     ITGFGRAEVVGRHCQENLLRHVSLDGRPLCETECPLSAALADGQPRESRVFLHHKTGH
                     RVPVDVRIGVLRDGASRVVGALEVFSDASNQAALEDQVEQLSQLAFLDALTQLPNRRF
                     VEVRLESACNELNRYGWTFGLLFVDVDHFKLINDVYGHEAGDDMLCMVARTLSAGLRP
                     SDVLARWGGEEFLAVIRNAEGGALEACAERCRSLVEHSGLRRGPGVPPVVVTVSVGGT
                     VARPGELPADMVSRADAAMYRSKNEGRNRVTI"
     gene            complement(964..1491)
                     /locus_tag="PLQ54_08105"
     CDS             complement(964..1491)
                     /locus_tag="PLQ54_08105"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOS93259.1"
                     /translation="MVEELVGVKDRIRSVPDFPAPGILFRDITPVLGAPELLRQSVEA
                     LTGYARSRRAEVIAGIESRGFVFGVPVALALGVPFVPIRKAGKLPADTVSAEYALEYG
                     TAKIEMHRDAFGPGRRVVIVDDLLATGGTAAAAAQLVSQLEGEVAGMAFLIELVSLNG
                     RSRLTAWDVASFVRY"
     gene            complement(1507..2310)
                     /locus_tag="PLQ54_08110"
     CDS             complement(1507..2310)
                     /locus_tag="PLQ54_08110"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOS93260.1"
                     /translation="MELRADIGVFGGSGFYEFAEDLRETKIETPYGATSDPVALGTVG
                     GRRVAFLPRHGKDHRLPPHMINYRANVWAMKSLGVRRIISPTAAGSLQAHIKPGEFVI
                     VDQFVDRTSGRKDTFYDGPVTTHIAGAEPYCPQLRAQAVRCAAELGIPCHDGGTVVVV
                     QGPRFSTKAESRWFTSNGWQVINMTQYPEVVLARELEMCYQAIALITDFDAGVVAEGE
                     AQPVSADAFLEVFRANIDNAKRLCEAVIRAIPNERTCACGMALQGARLD"
     gene            complement(2324..>3277)
                     /gene="mtnA"
                     /locus_tag="PLQ54_08115"
     CDS             complement(2324..>3277)
                     /gene="mtnA"
                     /locus_tag="PLQ54_08115"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOS93261.1"
                     /translation="PEICEAIRVLRVRGAPAIGCAAAYGLALRAVHGPEATPEELLPG
                     LESAARELAATRPTAVNLFWAIDRIMSLAGGRSWSSLDELRQALVDDARSIQAEDDAA
                     CRAIGAYGAGLLPDPCRILTHCNAGGLATSGYGTAVGVIRAAHEQGKRVRVWVDETRP
                     LLQGARLTAWELSQLEIPATLITDSMAAHVMARGQVDAVVVGADRIAANGDVANKIGT
                     QGVAILAEYFGVPFYVAAPLSTVDLSLPTGAGIPIEERSADEVTTPRGTRFAPELTAT
                     LTVYNPAFDVTPAHLIRAIVTEKGVLHPPYGPALARWAGSK"
BASE COUNT          612 a         1228 c          967 g          470 t
ORIGIN      
        1 gatcgtgacg cgattgcgac cctcgttctt gctgcgatac atggccgcat cggcgcgcga
       61 gaccatatcc gcgggaagct cgccaggacg cgccactgtg ccgcccaccg acaccgtgac
      121 gaccacggga ggaacaccgg gaccacggcg aagacccgag tgctccacga gtgaccggca
      181 tcgctcggcg cacgcctcaa gcgccccgcc ctcggcattg cggatcactg ccagaaactc
      241 ctcgccgccc catcgagcca gcacatccga cggccgcaga cccgccgaca gggtacgcgc
      301 gaccatgcat agcatgtcgt cccccgcctc gtggccgtag acgtcattga tcagcttgaa
      361 gtggtccaca tcgacgaaca gcagcccgaa agtccagcca taccggttca gctcgttgca
      421 ggccgactcc agacgcacct cgacaaaccg gcggttcgga agctgcgtca gcgcgtcgag
      481 aaacgccagc tgcgacagct gctccacctg gtcctcgagg gccgcctggt tcgacgcatc
      541 gctgaatacc tcgagtgccc ccaccacgcg actggcccca tcgcgaagga ccccaatccg
      601 cacatcaacc ggcacacggt ggcccgtctt gtggtgcagg aacacccggc tctcgcgagg
      661 ttgcccatcg gcgagcgcgg ccgatagggg gcactcggtc tcgcacagag gacgcccgtc
      721 caatgacacg tggcggagca ggttctcctg acagtgccgc ccgaccactt cggcgcgccc
      781 aaagcccgtg atccgctccg cggactggtt ccagaaaacg atggtccgat ccacgtccac
      841 cagataagcg ccctcaccga tcgagtcgag cagcgcctcg ggttgaagct cgcgcagcag
      901 tgcggcccct tgcctcggag tcactcgatc gcctccccgc cccgcgatgc ggtgtgatca
      961 cctctaatag cgaacgaatg aagccacgtc ccacgccgtc agtcgcgacc gcccgttcag
     1021 cgacaccagc tcaatcagga aggccatgcc ggcaacctcg ccctcaagct gactcacaag
     1081 ttgggcggcc gctgccgcag tcccgccggt ggccagcagg tcgtcgacga tgacaacccg
     1141 cctccccggc ccgaaggcat cgcggtgcat ctcgatcttc gccgtgccat actccagcgc
     1201 atactcggcc gagaccgtgt ccgccggaag cttacccgcc ttgcggatcg gcacaaacgg
     1261 aacccccaac gccagggcga ccggaacacc gaacacgaac ccccgcgact cgatgccggc
     1321 gatgacctcg gcccggcgcg atcgcgcata gcccgtgagg gcctccacgg actgacgcaa
     1381 cagctcaggg gcgcccagga cgggcgtaat atcccggaac agaatgccgg gcgccggaaa
     1441 atccggtacc gaacggatgc ggtccttgac gccgacgagc tcctccacca acgacaccct
     1501 cccagactag tcgagccgcg cgccctgcag cgccatccca catgcacacg tgcgctcgtt
     1561 ggggatcgcc cggatcacgg cctcacagag ccgcttggca ttgtcaatgt tcgcgcggaa
     1621 cacctcgagg aaggcatcgg cactcacggg ctgagcctcc ccctcagcca caacgcccgc
     1681 gtcgaagtcc gtgatcagcg caatggcctg atagcacatc tcgagttccc gggccaagac
     1741 aacctcgggg tactgagtca tgttaatgac ctgccagcca ttgctggtga accagcggct
     1801 ctcggccttg gtcgagaaac gcgggccctg gaccaccacc accgtgccgc catcgtgaca
     1861 cggaatcccc agctccgccg cacaccgcac cgcctgggcg cgaagctggg ggcagtacgg
     1921 ctcggcaccg gcgatgtgcg tcgtcaccgg gccgtcgtag aacgtgtcct tccgacccga
     1981 cgtgcgatcg acgaactggt ccacgatgac aaactcgccg ggcttgatat gggcctgcag
     2041 gctgcccgcg gccgtgggac tgataatgcg ccgcacaccc agcgacttca tggcccacac
     2101 gttggcacgg tagttgatca tgtggggagg cagccggtgg tccttgccat gccgcggcag
     2161 gaaagccacc cgccggcccc ccacagtgcc cagggccacc ggatcgctcg tggcaccgta
     2221 aggtgtctcg atcttggtct cgcgaaggtc ctcggcgaac tcgtagaacc ccgaaccacc
     2281 aaagacccct atgtccgcac gcaactccac gtctgccgct cctctacttg gatcccgccc
     2341 accgcgccaa cgccggtccg tagggcggat ggagcacgcc cttctcggtg acaatcgccc
     2401 ggatcagatg agccggggtc acgtcgaaag ccgggttgta caccgtcagt gttgccgtca
     2461 gctcgggggc gaaccgcgtc ccccgtggcg tcgtcacctc gtccgccgat cgctcctcga
     2521 tcgggatacc cgcccccgtg ggcagcgaga gatccacggt cgacaggggc gcggccacat
     2581 agaagggcac gccgaaatat tccgccagta tggcaacgcc ctgagtgcca atcttgtttg
     2641 ccacatcccc attggccgcg atgcggtcgg cgccgacgac caccgcatcc acctggcccc
     2701 tcgccatcac atgggccgcc atgctatcgg tgatgagcgt cgccgggatc tccagctgcg
     2761 acagctccca cgccgtcagc cgcgcgccct gaagcagcgg ccgggtctcg tcaacccaga
     2821 cgcgaacacg cttcccctgc tcgtgcgccg cgcgaatcac ccccacggcc gtaccatatc
     2881 cactggtcgc caggcccccc gcattgcagt gcgtcaggat tcggcacggg tccggcagca
     2941 gcccggcacc atacgcgccg atggcccggc acgcggcgtc gtcctccgcc tgaatggacc
     3001 gcgcgtcatc caccagggcc tgccgcagct catccaacga cgaccacgaa cggccgcccg
     3061 cgagcgacat gatgcggtca atcgcccaaa acagattcac ggccgtgggc cgtgtagcgg
     3121 ccagttcgcg cgcggccgac tccagaccgg gcagaagctc ctcaggcgtc gcttccgggc
     3181 catgaaccgc ccgcagcgcc agcccgtaag cggcggcgca gccgatggcc ggcgcaccgc
     3241 gaacgcgcag cacccggatc gcctcgcaga tctcggg
//
LOCUS       DAOSPL010000379         3274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29363_length_3274_cov_2.968002, whole genome shotgun sequence.
ACCESSION   DAOSPL010000379 DAOSPL010000000
VERSION     DAOSPL010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29363_length_3274_cov_2.968002"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="PLQ54_08120"
     CDS             complement(<1..986)
                     /locus_tag="PLQ54_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93262.1"
                     /translation="MPEVSQEILEECAYMYVEDLVHECIHSSDEPVRTNVIVLWLRER
                     GLSVSSSFIRAFFLGSERFVSDGRRWEVACRKEAEGAAIDGAARALLRGVGRPLPLRA
                     LAHQVALAKTMGPKETMVQVERLVRSRDSYFVTEEGMVGLSEWLLRVGSGSHEDVVLD
                     NFFEDSSEVDAISEAFDQIRRDDDSATMLAAMEVVGCPLTIRALQYLCWRRDPAAFDA
                     QAHYVALLRSDDFLVFSDQSAAPSSLRPPIIELIQAAAQEAAEEDGAAHEGLTGGSEF
                     RLSDEDMGEVIALLEQKRRSMSPDALVMEALEVFPEEERFAAAVARLEDALEV"
     gene            complement(1106..1915)
                     /locus_tag="PLQ54_08125"
     CDS             complement(1106..1915)
                     /locus_tag="PLQ54_08125"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HOS93263.1"
                     /translation="MLPLRVGTDTDNQSIAAAIAAAMERDLPPGAAARVAVDGPPGGL
                     GTGDGASITLCISGVEASAMPLPRLAPRGQSGMEGRLSLLRLRLEDVRLPEGALDLVE
                     MSFPGAGYWLGADGDRTHLRLVNLPGGTARVLLSTASLRRLVSARAPTIANPEIRLAD
                     GGWVEATGGIRLGPFPARARLRGRIVCRTGNALCIEDLELSAGSLALPAPLVRSVETA
                     VNPLVDLARDVPLPFDVRITQVTTEDGNLRLDARVSPLVADGAKKDWWQLS"
     gene            complement(1975..2844)
                     /locus_tag="PLQ54_08130"
     CDS             complement(1975..2844)
                     /locus_tag="PLQ54_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOS93264.1"
                     /translation="MPDLLARLSDRPLVADGATGTELQKRLGNPGECYEALNQTHPDV
                     VRDLAAAYAAAGSDIITTNSFGGSRLKLARVGLGSQARALSITAARLAREGAGSDVLI
                     AGSMGSTGRLIEPLGDLSEEEAVECFSEQAAALAEGGADLILVETMMSLEEALAAVRG
                     ARQAADIPVACTFAFVMSHRTMFGVGVEDAVRSLEQAGAAAVGFNCGELTLESALTLV
                     EEFAAVASVPVIAQPNAGLPELVAGETVFRETPESMGEYARRFVAAGAAIVGGCCGST
                     PEHIRSMVKALRP"
     gene            complement(2886..>3274)
                     /locus_tag="PLQ54_08135"
     CDS             complement(2886..>3274)
                     /locus_tag="PLQ54_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012365921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOS93265.1"
                     /translation="SAPRLVTEAMVAAMKPGAAIVDVAIDQGGCVETMRPTSHSNPVF
                     IHSGVVHYGVTNMPAAVPRTSTWALTNATMPYAQALAALSWERAAALDPALRRGIQIV
                     DGQVVYDAVAQSLNLPLGSLPASVAS"
BASE COUNT          566 a         1166 c         1015 g          527 t
ORIGIN      
        1 acctcgagcg catcctcgag gcgggccacg gcagccgcga acctctcttc ctcggggaat
       61 acctcgagag cctccatgac cagcgcatcg ggagacatcg atcgtcgctt ttgctcgagc
      121 agcgcgatga cctcacccat gtcctcatcg ctgaggcgga actcgcttcc ccctgtgagc
      181 ccctcgtgag ccgcaccgtc ttcttcagcc gcctcctgcg cggcagcctg gataagttcg
      241 atgatcggtg gacgcaggct gctgggagcc gcagactggt cggagaaaac gaggaagtca
      301 tcgcttcgca gtagcgccac atagtgggcc tgtgcatcga acgcagcggg atcgcggcgc
      361 cagcacaggt actgcagcgc gcggatcgtc aggggacagc cgactacctc catggccgct
      421 agcatggttg ccgagtcgtc gtcgcgccgg atctggtcga atgcctcaga tatggcatcc
      481 acctcggagg agtcctcgaa gaagttgtcg agcacgacat cctcgtggga cccggatccg
      541 actctgagca accattcgct cagccccacc atgccctctt cggtgacgaa gtatgagtcg
      601 cgggagcgca ccaaccgctc gacctgcacc atggtctcct tgggccccat ggttttcgca
      661 agcgcaacct ggtgggccag ggcgcgcagt ggcaggggcc ggcccacgcc gcgcagaagg
      721 gcccgagccg cgccgtcgat cgccgcgccc tcggcttcct tgcgacacgc gacttcccat
      781 ctgcgcccat ccgacacgaa ccgttcgcta ccgaggaaga atgcgcggat gaacgatgac
      841 gatacgctca gcccccgctc cctcagccac agaacgatga cgttcgtgcg gaccggttca
      901 tctgagctgt ggatgcactc gtggaccagg tcctcgacgt acatgtatgc acactcttcc
      961 aggatctcct gggacacctc aggcaataaa acacacacct ccccacgcgg gccacttgct
     1021 gggttctgtg acccacgcgc cacacacgcc taccatggcg atccggtttg ccgttcgcca
     1081 cattcgtctg tgtgatacgg ctatttcaag acaactgcca ccaatccttc ttggccccat
     1141 ccgccacgag ggggctcacg cgggcatcga gacgcaggtt gccgtcctcg gttgtcacct
     1201 gcgtgatgcg cacgtcgaac ggaagcggta cgtcccgcgc caggtccacg agtggattca
     1261 ccgccgtctc cacgctccgc accagaggag ccggcagtgc cagcgagccc gcgctgagct
     1321 ccaggtcctc gatgcacagg gcgttgcctg tccggcacac gatccgcccg cgcaagcgcg
     1381 ctcttgccgg aaacgggccg agccggattc ccccggtggc ttccacccag ccgccatcgg
     1441 ccaggcggat ctctggattc gcgatcgtag gtgctcgcgc cgacaccaac cggcgcaggc
     1501 tggcagtcga gagcaagacc cgcgcggttc cgccaggcag gttcacaagc ctcaggtgtg
     1561 tgcgatcgcc atcggcgccc aaccagtagc cggcaccggg gaaggacatc tcgacgaggt
     1621 caagcgcgcc ttcgggcaga cgtacatcct caagccgcag ccggagcagg ctgagccggc
     1681 cctccatgcc cgactgcccg cggggcgcca ggcgtggaag cggcatcgct gatgcctcga
     1741 cgcccgagat gcacaaggtg atgctggcgc catcaccagt ccccagcccc ccgggtggcc
     1801 cgtcgacggc gacccgagcc gcggcgcccg gaggcagatc ccgctccatt gccgccgcga
     1861 tcgccgcggc aatactctgg ttatcggtgt cggtccccac gcgaaggggc agcacgatct
     1921 ctgttcggga gtgcgattcc atcctggggc ctactcgggc gagagagtgc ggcactaggg
     1981 ccgcagcgcc ttcaccatac tgcggatgtg ctcgggtgtc gacccacagc agccccccac
     2041 gatggcggca ccggccgcca caaaccgcct ggcatactcc cccatggact ccggtgtctc
     2101 gcggaacacc gtctcgccgg cgacaagctc aggcagaccc gcgttcggct gggcgatcac
     2161 cggtaccgaa gcaactgctg cgaactcctc gaccaatgtc agcgcgctct ccaatgtgag
     2221 ttcgccgcag ttgaacccca cagcggccgc gccggcctgc tccagcgacc ggacggcgtc
     2281 ctccaccccc acgccgaaca tggtgcggtg gctcatcacg aatgcgaagg tgcaggcgac
     2341 tgggatatcg gccgcttggc gcgcccctcg caccgccgcg agggcctctt cgagcgacat
     2401 catcgtctcc acgaggatca ggtcggcccc gccttcggcc aatgccgccg cctgctcgga
     2461 gaagcactcc acggcttcct cctcggacag gtcgcccagc ggctcgatca ggcgccccgt
     2521 ggagcccatg gaaccggcga tgagcacatc ggacccggcg ccctctcggg ccaagcgcgc
     2581 tgcggtgata ctcagtgcgc gagcctggct gcccagtccc acccgggcga gcttgaggcg
     2641 cgatccaccg aagctgttgg tcgtgatgat gtccgaccct gcggcggcat aggcggccgc
     2701 gaggtcacgg acgacgtcgg gatgggtctg attcagtgcc tcgtaacact cacccgggtt
     2761 gcccaggcgc ttctgaagct ccgtgcccgt ggccccgtcg gccacgagtg gcctatcact
     2821 cagacgcgcc agcagatctg gcatttcgtg tcctccctct ccccatcgcc agcggcaccc
     2881 cagacctacg aggccaccga tgccggcaac gacccaagcg gcaggttcag cgattgggcg
     2941 actgcatcat acacgacctg gccgtcgacg atttggatcc cgcgtctcag ggccgggtcg
     3001 agggcggccg cgcgctccca actgagggcg gccaaagctt gcgcgtaggg catcgttgca
     3061 ttggtcagcg cccatgtgga cgtacgcggc acggcagcgg gcatgttggt cacgccgtag
     3121 tgaacgacgc cactgtggat gaagaccggg ttcgagtgcg acgtcggccg catggtctca
     3181 acgcagcctc cctggtcgat agcgacgtcc acgatggccg cgccgggctt catggcggcc
     3241 accattgcct cggtcacgag ccgtggcgcc gagg
//
LOCUS       DAOSPL010000380         3271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29391_length_3271_cov_3.400187, whole genome shotgun sequence.
ACCESSION   DAOSPL010000380 DAOSPL010000000
VERSION     DAOSPL010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29391_length_3271_cov_3.400187"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="PLQ54_08140"
     CDS             complement(<1..1103)
                     /locus_tag="PLQ54_08140"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HOS93266.1"
                     /translation="MSELIERALRDATETRLAIIGEGAVERVGEAFKQLFGDATAIVV
                     GDERTMAVAGDRVVEVLREAGVPLLESYVFPGDPELYASYDNVEVIRDALAELDATAV
                     AVGSGSLNDLVKRAVGELGRPYLQVGTAASMDGYTAFGASITNKGFKITHDCPAPAGS
                     ISDTAIMAAAPQVMTASGFGDLIGKVPAAADWILADAVGVEPIEQNVWDLVQIPLRAS
                     LAHPDLLAKGDVAAISDLAEGLIMSGLAMQAMQSSRPASGAEHQFSHLWEMEGLGLHT
                     KPRRLSHGFKVGLGTVCVSALYERFLARDITQLDTATAVAKWPSREELEAKVRAAFPI
                     PGMVDDVVGQQLGKWVTPEELADRIELLKRAWP"
     gene            complement(1118..2674)
                     /locus_tag="PLQ54_08145"
     CDS             complement(1118..2674)
                     /locus_tag="PLQ54_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOS93267.1"
                     /translation="MDDTYVLGIDYGTESCRVALFDLAGRPLTFAATPYPTTHPRAGW
                     AEQNPDDWWQALRVSARRALAASGVRPEQIVGIGSDATTMTVVAMDADGRELRPAIMW
                     MDVRATEQAARAASSTSVARRYNGGGTAPATAEWYPFKAAWLREREPETYRRARYLLD
                     APDWLTHKLTGEWTVNINSAALRMYYDRDHGGWPTDFYEHVGCGDVFDKIPDRVVALG
                     VPVAGLSADAAEWLGLRAGTPVAQGPADAWAGQIGLGVVTPGRMALITGSSHVLTGQT
                     DRPASGRGFFGAYTDGVVPGQYTIEAGQASTGSVLKWFKDGFAPDAAAEAERRGVSVY
                     DLLNDRAAGIRPGCDGLIVNEYFQGNRTPYTDSRARGMFWGLSLAHTPAHLYRAIQEG
                     ICFGTAHNLGVMEEAGVRVDELVACGGFTKSRSLMQLHADIAGKPITLTEVGDAVALG
                     AAMVAAVGAGVYGDLQQAAAAMVHDRETIAPDPDRHEEYAYYLGLYRSAYPRMADLNH
                     QLVEHETSSY"
     gene            complement(2717..3187)
                     /locus_tag="PLQ54_08150"
     CDS             complement(2717..3187)
                     /locus_tag="PLQ54_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HOS93268.1"
                     /translation="MGFRIVLACDDAGVEYKEILKADLEADPRVDEVVDVGVFSSEDK
                     SPYAHAAVEGARLIAAGRADRGLFICGTGMGVAMSANKVPGIRASVAHDSFSVERLVK
                     SNNAQVLTLGARVIGIELARRLAKEWLGYEFDPASPSAPKVAAIDSYDRPTAAE"
BASE COUNT          494 a         1153 c         1120 g          504 t
ORIGIN      
        1 tcaggccacg cccgcttcaa caactcgatc cgatcggcca gctcttcggg ggtgacccac
       61 ttccccagct gctggccgac cacgtcgtcc accatccccg ggatcgggaa ggcggcacgc
      121 accttggcct cgagctcctc gcggctgggc cacttggcca cggcggtcgc cgtatcgagc
      181 tgggtgatgt cgcgggcaag gaagcgctcg tagagcgcgg agacacacac ggtgccgaga
      241 ccgaccttga agccgtggct gaggcgtcgg ggtttcgtgt ggagtccgag cccctccatc
      301 tcccaaagat ggctgaactg gtgctcggcg cccgaggcgg gccgcgacga ctgcatcgcc
      361 tgcatcgcga gacccgacat gatcagaccc tcggcgaggt cgctgatcgc cgccacgtcg
      421 cccttcgcga gcaggtcggg gtgggccagc gaggcgcgca acgggatctg gaccaggtcc
      481 cagacgttct gctcgatggg ctcgacgccg acggcgtccg ccaggatcca gtccgcggcc
      541 gccggcacct taccgatgag gtcgccgaag ccggacgccg tcatcacctg cggcgccgcg
      601 gccatgatgg cggtgtcgga gatgctcccg gcgggtgccg ggcagtcgtg ggtgatcttg
      661 aaacccttgt tcgtgatcga ggcgccgaag gccgtgtacc cgtccatgga cgccgccgtg
      721 ccgacctgca ggtaggggcg tcccaactcg ccgacggcgc gcttcaccag gtcgttcaac
      781 gaaccggagc ccaccgccac ggccgtggcg tcgagctcgg cgagggcgtc ccggatcacc
      841 tcgacgttgt cgtagctggc gtacagctcc gggtcgccgg ggaagacgta ggactcaagc
      901 aggggcacgc ccgcctcgcg gaggacctcg accacgcggt cgcccgccac ggccattgtc
      961 cgctcgtcgc cgacgacgat cgcggtggcg tccccgaaca actgcttgaa cgcctccccc
     1021 acccgttcga cggcgccctc accgatgatg gcgagtctcg tttcggtggc gtcccgcagt
     1081 gcccgctcga tgagctccga catggtgtgt gccctttcta gtaggaagac gtctcgtgtt
     1141 cgacaagctg gtggttgagg tcggccatcc ggggataggc cgaacggtag aggcccaggt
     1201 agtacgcgta ctcctcgtgc cggtccggat cgggcgcaat ggtctcgcgg tcgtgcacca
     1261 tcgccgccgc ggcctgctga agatcgccgt agacgcccgc tcccaccgcg gcgaccatgg
     1321 ccgccccgag ggccacggcg tccccgacct ccgtgagggt gatgggcttg cccgcgatgt
     1381 cggcgtggag ttgcatgagg ctgcggctct tggtgaagcc cccgcaggcc accagctcgt
     1441 cgacccgcac gcccgcctcc tccatgaccc ccaggttgtg ggcagtcccg aagcagatcc
     1501 cctcctggat ggcccgatag aggtgcgcgg gggtgtgggc gagagacagc ccccagaaca
     1561 tgccacgcgc ccgcgaatcg gtgtatgggg tgcggttgcc ctggaagtac tcgttgacga
     1621 tcagcccgtc gcagcccgga cggatgcccg cggcccgatc gttgagcagg tcataaacgc
     1681 tcacgccgcg gcgttcggcc tcggcggcgg cgtccggggc gaagccgtcc ttgaaccact
     1741 tcagcaccga accggtcgat gcctggccgg cctcgatcgt gtactggccg ggaacgaccc
     1801 cgtcggtgta ggcgccgaag aacccgcggc ccgacgccgg gcggtccgtc tgacccgtga
     1861 gcacgtgcga ggagccggtg atgagggcca tgcggccagg ggtgacgacc cccaatccga
     1921 tctgcccggc ccaggcgtcc gccggaccct gcgccaccgg cgtcccggcc cgcaggccga
     1981 gccattcggc ggcgtccgcg ctgaggccgg ccaccgggac gcccagggcg acgacccggt
     2041 cggggatctt gtcgaagacg tctccgcagc ccacatgctc gtagaagtcc gtcggccagc
     2101 cgccgtgatc ccggtcgtag tacatgcgca gggccgccga gttgatgttg acggtccact
     2161 cgccggtgag cttgtgggtc agccagtccg gggcgtcgag gaggtacctg gcccggcgat
     2221 aggtctccgg ctcacgctcg cgcaaccatg ccgccttgaa ggggtaccac tccgccgtcg
     2281 cgggggcggt gccgcccccg ttgtagcgcc gggccaccga cgtcgaggac gccgcccgcg
     2341 cggcctgctc ggtcgcgcgc acgtccatcc acatgatcgc cggacgcagt tcgcgaccgt
     2401 cggcgtccat cgcgacgacg gtcatcgtgg tggcgtccga cccgatgccg acgatttgct
     2461 ccggccggac gccggacgcc gcgagcgcgc ggcgcgccga gacgcggagt gcctgccacc
     2521 agtcgtcggg gttctgctcg gcccacccgg cgcgagggtg cgtcgtcgga tagggagtcg
     2581 ccgcgaacgt cagcggccga cccgccaggt cgaacagggc gacccggcaa ctctcggtgc
     2641 cgtaatcgat gccgaggacg tacgtgtcgt ccatctggtc cccctctccg cgcctcggcc
     2701 tccgccttgt cctgagctac tcggcggcgg tcggccggtc gtaggaatcg atcgccgcca
     2761 ccttcggagc ggacggcgag gcgggatcga attcgtagcc cagccattcc ttggcgagcc
     2821 gacgtgccaa ctcgatgccg atcactcggg caccgagtgt gagtacctga gcgttgttcg
     2881 acttcacaag gcgttcgacc gagaacgagt cgtgcgcgac cgacgcacgg atcccgggaa
     2941 ccttgttcgc cgacatggcg acgcccatgc cggtaccgca gatgaagagg ccacgatcgg
     3001 cccggcccgc cgcgatcagg cgcgcaccct cgacggcggc gtgcgcatag gggctcttgt
     3061 cctcggaact gaagacgccg acgtccacga cctcgtcgac ccgggggtcg gcttcgaggt
     3121 cggccttcag tatctccttg tactcgacgc ccgcgtcatc gcaggcgagc acgatgcgaa
     3181 atcccatggc aaagtccttt caagtcgaac gagggcgaag cggaccagga accggcccgt
     3241 cctaccggat ccggaggccg gtttcggcgt c
//
LOCUS       DAOSPL010000381         3267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29431_length_3267_cov_3.743773, whole genome shotgun sequence.
ACCESSION   DAOSPL010000381 DAOSPL010000000
VERSION     DAOSPL010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29431_length_3267_cov_3.743773"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLQ54_08155"
     CDS             <1..250
                     /locus_tag="PLQ54_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93269.1"
                     /translation="RVLEGVASDPEVVVREIPTERHGTYYAMVNTSMSRKDAVRVKLG
                     AIGAITFAASSEPLVARDGALELSLYPGELLAVHVAAP"
     gene            complement(413..2137)
                     /locus_tag="PLQ54_08160"
     CDS             complement(413..2137)
                     /locus_tag="PLQ54_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOS93270.1"
                     /translation="MAKPTLEDDSLIEGPLVAEIVFEGLRGVSEEEARQTIALEIGRK
                     FTPARGDADLRRLLALGAFRTRAEESYVHTEPVGDQVRVIFHLTENPAVRGIVFEGNT
                     VLSAEALTAAVQGVVRPGDVYRTDIGGKVAEQASGAYLDAGYAALVLHPTLDDAGVLH
                     VPLIEQYIEDVEIEGLRKTKRKLVMSEIRTRPGQLYNLGLIRRDLDRLIDLGLFDSVA
                     VADPRPGTQTGAVILVYQLDERKTGTFDAGLGYGPKDGLIGYVAVSENNLGGHGRKLG
                     VRAEFGGTYAFDLHFSDPFLDSHHTSIELDVYSRRTRTDLVGFAGLSSPNQVFGSETH
                     TGGVVSLSRPLDRDRKRLVNVSYMNEYVNNQDARLINILDPSALRGRVGKLSAGFIND
                     SRDMPSNPARGGREAIQIEQAAGVLGSSFTFTKFGVDLRRYFEIGDRSVIALRGMYGL
                     VRGQNTPIFEAFRAGGMDTIRGYREDRFVGSNVVLLNAEYRYRLFGDDPRKSLQALAF
                     VDYGDAYGGTWPGLGNAVFYSEHQQFTGNLGVGVGLRWNTRLGPIGVDYGVGREGGRG
                     YLTFRQTF"
     gene            complement(2733..>3267)
                     /locus_tag="PLQ54_08165"
     CDS             complement(2733..>3267)
                     /locus_tag="PLQ54_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOS93271.1"
                     /translation="ENPRRGGLHTASLEYADRWLGGASRFSKFEVDLRQYFSSGDKGV
                     FALRGNYGSALGSEPPVYEAFRVGGADTVRGYLEDRWYGMNRLLVSAEYRYQISGHSA
                     RSGIQLVLFADYGDAYGGRWASQDGSILFAEHQTFQGSLGYGLGVRVNTPLGPIRLDY
                     GIGSEGGRTHFGISQTF"
BASE COUNT          555 a         1135 c         1065 g          512 t
ORIGIN      
        1 ccgggtgctc gaaggcgttg cttctgaccc tgaggtcgtc gtccgcgaga tccccaccga
       61 gcgccacggc acctactacg ccatggtcaa tacgagcatg tcacggaagg acgcggtccg
      121 cgtcaaactc ggggccatcg gcgccataac gttcgcggcc tcgagcgaac cgctggtggc
      181 tcgcgatggc gcactggagc tatcgctcta tcctggcgag cttctggccg tgcatgttgc
      241 cgcgccgtag gctcatcgtt acgagcctgg gggtggggcc gcaggtacct cgagcgcgga
      301 tgcggacatg atgggcgggc cacacggaga cgaccgctcg ccgcaggcgg atacggggcc
      361 ccaaagggtc cacatgccgc gcacggcggt ggcggtacct tcccccactc gcctagaacg
      421 tctgccggaa tgtcagatag ccccggccgc cctcgcgccc cacgccgtag tccaccccga
      481 tgggcccgag gcgcgtgttc cagcgcagac caacaccgac accgaggttc ccggtgaact
      541 gctgatgctc ggagtagaac accgcgttcc cgaggccggg ccacgttccg ccgtaggcat
      601 cgccgtagtc gacgaaggcc agcgcctgca ggctcttgcg aggatcatcg ccgaacaggc
      661 ggtagcggta ctcggcattc aggagcacca cattcgagcc gacgaaccgg tcctcgcggt
      721 agccccggat cgtgtccatg cctccggccc tgaaggcctc gaagatgggc gtgttctgcc
      781 cacggaccag cccgtacatg cctcggagcg ctatgacgct gcggtcgcca atctcgaagt
      841 accgacggag gtcaacgcca aatttggtga aggtaaatga actacccagg acgccggcgg
      901 cctgctcgat ctggatggcc tcacgaccac cccgagcggg gttggacggc atatcgcgcg
      961 agtcattgat gaacccagcc gagagcttgc ccacccgccc cctaagggcc gacggatcca
     1021 ggatgttgat caaccgcgcg tcctggttgt tcacatactc gttcatatag gacacgttca
     1081 ccaggcgctt gcggtctcgg tccagaggcc gcgacagcga caccacccct cccgtgtgag
     1141 tctccgaccc aaacacctgg ttgggcgagc tcagacccgc gaaccccacc aggtccgtcc
     1201 gggtccgccg tgaatacacg tcgagctcga tggatgtgtg atggctgtcc aggaacgggt
     1261 ccgagaagtg taggtcaaag gcataggtcc cgccgaactc ggctcgcacg cccagcttcc
     1321 ggccatgtcc ccccaggttg ttctcggaga cggccacata gccgatgagt ccgtccttgg
     1381 ggccgtagcc gagaccggcg tcgaatgtcc ccgtcttgcg ctcatccagt tggtagacca
     1441 ggatcaccgc cccggtctgt gtgccgggac gcgggtcggc gacggcgacc gagtcgaaca
     1501 gccccaggtc gatcagccgg tccaggtcgc gccggatcag tcccaggttg tagagctgcc
     1561 cgggacgtgt ccggatctcg gacatcacca gcttgcgttt ggtcttccgg agtccctcga
     1621 tctcgacgtc ctcgatgtac tgctcgataa gcggcacgtg caggacgccc gcgtcgtcca
     1681 gcgtgggatg cagcacaagg gccgcgtacc cggcatccag ataggcgccc gaagcctgct
     1741 ccgccacctt gccgccaatg tcggtgcggt agacgtcgcc aggccggacc acgccctgaa
     1801 ccgcggcggt cagggcctcg gccgacagca cggtattgcc ttcgaagact atgccccgta
     1861 ccgccgggtt ctccgtgagg tggaagatca ctcgcacttg gtcccccacg ggctcggtgt
     1921 gaacatacga ctcctcggcg cgagtccgga acgctcccag agccagcagg cggcgcaggt
     1981 ccgcgtcccc gcgggccggc gtgaacttgc ggccgatctc gagagcgatc gtctgccggg
     2041 cctcctcctc gctcacaccg cgcaggccct cgaacacgat ctcagccacc agcggcccct
     2101 cgatcaggct gtcgtcctcg agggtgggct tggccatggg cgagggcacc gactggcccg
     2161 gagggctcat cacggccccg gccggttgcc ctggcggaag ccctggtgtc tcgccaggcg
     2221 gcggggcggc cggtggctca ttggccaggc cttcttcccc atcagggacg ggctcggggg
     2281 gaacagcggg cggcggaagg gcctcgccaa cctcgccggg tacggccggc gggaccggct
     2341 ccggagctgg tgtgtcgggt ggcggagtgg gtcgggtcgc atcccctggc gtcctcggcg
     2401 gcgccttgat acccggaacc gcctcgcaac gatccgcgga cagcatcatg gccccggcaa
     2461 ggaggccgac aagtgacacg gtgacgaaga gagcccgtac cgatcggctc ccggctgatg
     2521 caggcagtcc catgtgggcc ccgcctcccc ttcaaactgc cgagtctcgc caggcgtgag
     2581 actcgactgc cccgggcccc gtgttggggc cccgaggcct ggcagccggg gtaggggtct
     2641 gggccagacg gccgtagacc cccgggcggg tcctgactcc cctggcggcg agatcgcttc
     2701 gggccggact cacggccccc tcgccgcacg tcctagaagg tctggcttat cccgaagtgc
     2761 gttcgaccgc cctcggagcc gatgccgtaa tccaggcgga tcggcccgag aggcgtgttg
     2821 acccgaacgc cgagcccgta gcccagggag ccctggaagg tctggtgctc ggcgaacaag
     2881 atcgaaccat cctgcgatgc ccaacgccca ccataggcat cgccataatc cgcgaacagg
     2941 acgagctgaa tgccgctgcg ggcactatgc ccggagatct ggtagcggta ctcggcgctg
     3001 accagcagcc ggttcatacc gtaccagcga tcctccagat agccccggac cgtatcagcc
     3061 ccaccgacgc ggaacgcctc gtagacgggc ggctcgctgc cgagcgcgct gccgtagttg
     3121 ccgcgcagcg cgaagacgcc cttgtcaccc gaagagaagt actggcgcag atccacttcg
     3181 aacttcgaga agcgcgaagc gccgcccagc caccggtccg cgtactccag cgatgcggtg
     3241 tgcagaccgc cacggcgagg gttctca
//
LOCUS       DAOSPL010000382         3262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29491_length_3262_cov_3.432180, whole genome shotgun sequence.
ACCESSION   DAOSPL010000382 DAOSPL010000000
VERSION     DAOSPL010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29491_length_3262_cov_3.432180"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..752)
                     /locus_tag="PLQ54_08170"
     CDS             complement(144..752)
                     /locus_tag="PLQ54_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOS93272.1"
                     /translation="MTPPAEDQSEATVAVNTGPSVCPVAGAARDLTLGGLFGALAIVL
                     PIVFHAVGMGKVFLPMYLPILALGLLASWRTAALVGLLVPLISAILTGMPPLAPPIAF
                     IMMAELAALGLIASVARSVGLGIWLAAVLGLLAARAVGVLALLTIGRALGYEQGLYEF
                     AVAGFVASWPGIALQLTVVPSAVYLIERTSIFGRPARGMRCV"
     gene            complement(736..1395)
                     /locus_tag="PLQ54_08175"
     CDS             complement(736..1395)
                     /locus_tag="PLQ54_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOS93273.1"
                     /translation="MARRGFTLIELLVVIAIIAILAAILFPVFAKAREKARQASCASN
                     MRQIGLAFFMYTSDYDGTFPMVYQWKTNLQPYVKNTQVNYCPSRPNLPWYYGQGYNIG
                     CSSPPVTGVPLASESIIVSPSQKILVAEWDRCNAGPPCGPVGLFSGGATCYWAVTRIH
                     NGGSHLLFCDGHVKWFRPDEYHSTTEYADSAGQPVPSTVTVVPEPQWRKYWDVTYDAA
                     S"
     gene            complement(1477..2394)
                     /locus_tag="PLQ54_08180"
     CDS             complement(1477..2394)
                     /locus_tag="PLQ54_08180"
                     /inference="COORDINATES: protein motif:HMM:NF021501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HOS93274.1"
                     /translation="MTAIDVARKPWLVERLSRLLGGQPLLVERYLNQFGLVLSPHYVA
                     EIAGAPPGKDPIFHAVGRCPVCDQPVSTWTVRASTQRLTTNEFQVVTRAEGTQGHASV
                     NPVLYDVTGCQACLYVSSARSEFKSETGSDSSSPLADRVKKGLDGARAEREAVLVEIP
                     FESIFDRPRDLTSAMLAYQMAVRCEQVKVGFDVPGSHMRLASTRFRLATLEDLAGRTD
                     SANETIAAALTDVRDAYSRTEDPRQAAPAAYLALAAAIRLGEASVASEYLQVLRGLEK
                     TLQADPKLKSSLRWCSQARDLWTDWRQGQ"
     gene            complement(2536..>3262)
                     /locus_tag="PLQ54_08185"
     CDS             complement(2536..>3262)
                     /locus_tag="PLQ54_08185"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOS93275.1"
                     /translation="NLLVNAGFETLDADGRPAGWAPVWARDAGTATVTVETTGAPEGT
                     RCVKVTHTGSQDWSFAFPDRFAVRPMDILRISAWVRSQDLAGSAEIGVVVTDARGEAL
                     NWVYGARRVPRSAEWSRIGGRLVMPPGANHIQFRLTGAGIGTVWIDDAELRRVGNVDD
                     LRAQWPAEDASVETESVRVRLDARTGVIAVTDHRTGIVWRQAAAGTSVVLREVRTVGN
                     VLTIAMEDVANDLVIRATIAAKP"
BASE COUNT          605 a         1086 c         1092 g          479 t
ORIGIN      
        1 caacctcaat gccaaaatgc gcctgggcct gcagccgatt caccgcgaac cggccgcacc
       61 gcaggccagc gacaacatag ggtcccacgt ggccgtggaa ggcgttgagg atggcgagat
      121 cgctctccga gaccgcggca gtgtcagaca cagcgcattc ccctcgcggg ccgcccgaag
      181 atgctggtgc gctcgataag gtagacggcg ctcggcacga ccgtcaactg caaggcgatg
      241 ccgggccagg aggcaacgaa cccggcgacg gcgaactcgt agaggccctg ttcgtagccg
      301 agggcccggc cgatggtcag caacgccaac acgccgacgg ccctggcggc caagagaccg
      361 aggaccgccg ccagccagat ccccaacccc acagatcggg ccacactggc gatcagcccg
      421 agcgccgcca actcggccat catgatgaaa gcaatggggg gcgccagggg gggcatgccc
      481 gtcaggatcg ccgagatcag aggcacgagc agcccgacca gagcagccgt ccgccacgag
      541 gcgagtagcc ccagagccag gatagggagg tacatgggca ggaacacctt gcccatgccc
      601 acggcatgaa acacgatggg caggacgatg gccagggctc caaagagccc tccgagggtc
      661 aggtcacgcg cggcgccggc gaccggacat accgacgggc cggtgttcac agccaccgtc
      721 gcttcagact gatcctcagc tggcggcgtc ataggtcaca tcccagtact tgcgccactg
      781 tggttccggg accaccgtca cggtcgaggg gaccggctgg ccggcgctgt ccgcgtactc
      841 ggtggtgctg tggtactcat cgggccggaa ccacttgacg tggccgtcac agaacagcag
      901 gtgcgagccg ccgttgtgga tccgcgtgac cgcccagtag cacgtagcgc cgcccgagaa
      961 cagaccaacc ggcccgcacg gaggccccgc gttgcaccgg tcccactcgg cgaccaggat
     1021 cttctgcgag ggggacacga tgatgctctc gctggccagc ggaacgccgg tcaccggggg
     1081 cgaactgcaa ccgatattgt agccctgtcc atagtaccac ggcaggttcg gccggctcgg
     1141 gcagtagttg acctgcgtgt tcttcacgta gggctgcaag ttggttttcc actgatagac
     1201 catggggaac gtgccgtcgt agtcggaggt gtacatgaag aaggcgagcc cgatctgccg
     1261 catgttgctg gcgcagcttg cctgccgcgc cttctcacga gccttggcga agacggggaa
     1321 gagaatggcg gcgaggatcg cgatgatggc gatcaccacg aggagctcga tgagggtgaa
     1381 cccgcggcgg gccatggtgg cctcctagta acacgatttc gacagtcgta acaccactgt
     1441 acgagccaaa agcgcggatg tcaatcgcgc gaatggtcat tgcccctgac gccagtcggt
     1501 ccagagatcc cgggcttggg aacaccatcg gagcgaggac ttgagtttgg ggtcggcctg
     1561 gagagtcttc tcgaggccac ggaggacctg gaggtattcg gaggccacgc tggcttctcc
     1621 gagccggatc gcggcagcca gagccaggta cgcggcgggt gcggcctggc gcgggtcttc
     1681 ggtccggctg taggcgtcac ggacgtccgt cagcgcggcc gcaatggtct cgttggccga
     1741 atccgtgcgc cccgcgaggt cctcgagcgt ggccagacgg aagcgggtcg acgccaggcg
     1801 catgtgcgaa cccggtacgt cgaatccgac tttcacctgc tcgcaccgca cggccatctg
     1861 gtacgcgagc atggccgaag tgaggtcccg agggcggtcg aagatggact cgaaggggat
     1921 ctcgacgagc acggcctccc gctcggcccg cgcaccatcg aggcccttct tgacgcggtc
     1981 cgcaagcggc gaggacgagt cggatccggt ctcgctcttg aactccgacc gggcactgga
     2041 cacatagagg caagcctggc aaccggtaac gtcgtagagc acggggttga cactggcatg
     2101 cccctgggtg ccctcggccc gcgtgaccac ctggaactcg ttggtcgtca ggcgctgcgt
     2161 actcgctcgc acggtccagg tggagaccgg ctggtcgcag accgggcaac gccccacagc
     2221 gtggaatatg gggtccttac ccggaggggc gcccgcgatc tcagccacat agtgaggcga
     2281 gagcaccagc ccaaactggt tgaggtagcg ctcgaccagg agaggctggc ctccgaggag
     2341 ccgagagagc cgttccacca gccaaggttt tcgggctaca tcgatcgcgg tcatgggcag
     2401 ccctccccgg cagggcctgg cacgacggct gcgccggcca ggcattcgag ttcgagggtt
     2461 ccacagcgtg accgctgcct gagcagctgc gagtaacaac tcccaaaaca acggtagact
     2521 cgacggaggc gcccgctacg gcttggcggc gatagtcgca cggatcacga ggtcgttggc
     2581 aacatcctcc atggcaatgg tcaggacatt ccccacggtg cggacttcgc gcaacaccac
     2641 agaggtaccc gccgccgcct gtcgccagac gatgccggta cggtggtcgg tcacggcaat
     2701 gactcccgtg cgagcgtcga gccgcacccg gaccgactcc gtctcgacgg aggcgtcctc
     2761 cgcaggccac tgcgcccgca ggtcatcgac gttgccgacc cgtcggagct ccgcgtcatc
     2821 gatccatacc gtgccaatgc ccgcacccgt gagcctgaac tggatgtggt tcgcccccgg
     2881 tggcatcacg aggcggccgc cgatccggga ccactcggcc gatcgcggca cgcgccgggc
     2941 gccgtagacc cagttcaggg cctctccgcg ggcatccgtc accaccacgc cgatctctgc
     3001 cgagccagcc agatcctgac tgcgcaccca tgccgagatc cggaggatgt ccatggggcg
     3061 caccgcgaac cggtcgggga aggcgaagct ccagtcctgg ctcccggtgt gcgtgacctt
     3121 cacacaccga gtgccctcgg gcgctccggt ggtctcgacg gtgacagtcg ccgtccctgc
     3181 atcacgcgcc cacaccgggg cccagcccgc gggacggccg tcggcgtcga gggtctcgaa
     3241 gcccgcgttg acaagcaggt tc
//
LOCUS       DAOSPL010000383         3261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29503_length_3261_cov_3.690892, whole genome shotgun sequence.
ACCESSION   DAOSPL010000383 DAOSPL010000000
VERSION     DAOSPL010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29503_length_3261_cov_3.690892"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /gene="ilvC"
                     /locus_tag="PLQ54_08190"
     CDS             complement(<1..954)
                     /gene="ilvC"
                     /locus_tag="PLQ54_08190"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOS93276.1"
                     /translation="MAKLYYDDDASLDALKDKTIAIIGYGIQGRGQGLNLRDSGCKVV
                     VADIPGSPAWENAKADGQTLLSTEEAAEAGDIVQILTEDHVQARVYETQVKAHLKDGN
                     ALFFSHGFNIHYNQIVPPKTIDVLMVAPKGPGSLVRQQFEKGGGVPCLLAIHQDYTGK
                     AKEIGLAYAKAIGGTRGGVFETTFREETETDLFGEQAVLCGGLSALIKAGFDTLVEAG
                     YQPEMAYFECCHELKLIMDMVFKYGISGMRKRVSNTAAYGDMSRGPRVIDDYVREVMR
                     EMLREVQTGQFAKEWILECMAGRPVFKRVWEQDSEHPIESIG"
     gene            1239..2549
                     /locus_tag="PLQ54_08195"
     CDS             1239..2549
                     /locus_tag="PLQ54_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOS93277.1"
                     /translation="MLQSSLALLGGSKAVQSDPGDLFAWPIITQEDEDACLEVLRRGA
                     MSDTDVTREFEREFAAWHGIEYALAHSSGTAALEAAMWASGVRAGDEIICPSLTYWAS
                     ALPAFCLGATVVFAEVQAHSMCLDPNDIEHRITDRTRAIVAVHYCGHPCDMDPIMSIA
                     ERHGVKVIEDVSHAHGGEYHGRLLGTIGHCNAMSTMSGKALPVGEGGFLLTRDRLMYE
                     RAVAWGHYERTAEVLRDPSLAPYTGLPWGAHKYRMHQLSSAVGRVQLRHYRSRMEEIR
                     RSMDHFWDLLEGVPGVRAHRPPKGSGSHNGGWYAPHGMYVPEELGGLPVGRFCEAVQA
                     EGAPTHPGANLLLHLHPLLNDADIYGHGKPTRIAHAGRDVRQPSGSLPVTESIPERTY
                     AVPWFKHFRPSVIAEYALAFRKVAEHADDLLGAPERPGDPECRG"
     gene            2981..>3261
                     /locus_tag="PLQ54_08200"
     CDS             2981..>3261
                     /locus_tag="PLQ54_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93278.1"
                     /translation="MRARRGKGLLAADEVRLSRRIRRGACSVRYDPDTCTIHLIPSAP
                     LRADTLYAVWRADLTGIPSGPGAEGLPVEPFILEFRTAGNAPRRPNAPLP"
BASE COUNT          534 a         1117 c         1047 g          563 t
ORIGIN      
        1 cccgatgctc tcgatgggat gctcgctatc ctgctcccac acccgcttga agaccgggcg
       61 gccagccatg cattcgagga tccactcctt ggcgaactga cccgtctgga cctcgcgcag
      121 catctcgcgc atgacctcgc ggacgtaatc atcaatcacc ctggggccgc gactcatgtc
      181 gccatacgcc gccgtgttgg acacgcgctt gcgcatgccc gaaatcccat acttgaacac
      241 catgtccatg atcagcttga gttcatggca gcactcaaag taggccatct cgggctgata
      301 gccggcctcg accagggtgt cgaaccccgc cttgatcaga gcggacagtc caccgcacag
      361 cacggcctgt tcaccgaaca ggtcggtctc agtctcctcg cggaacgtgg tctcaaacac
      421 accgcctcgc gttccgccga tggccttggc gtaggccagg ccgatctcct tggccttgcc
      481 ggtgtagtcc tgatggatgg ccagaaggca cgggacgccg ccgcccttct cgaactgctg
      541 ccggaccaga ctgccggggc ccttcggcgc taccatcagc acgtcgatgg tcttgggggg
      601 gacgatctgg ttgtagtgga tgttgaaccc gtggctgaag aacagcgcgt tgccgtcctt
      661 gagatgggcc ttgacctggg tctcgtacac gcgagcctga acatgatcct cggtcaggat
      721 ctgcacgatg tcgcccgctt cagcggcttc ctcggttgag agaagcgtct gcccatcggc
      781 cttggcgttc tcccaggccg ggctaccggg gatgtcggcc acgacgacct tgcagccgga
      841 atcgcgcagg ttcaggccct ggccccgccc ctggataccg tagccgatga tcgcgatggt
      901 cttgtccttg agcgcatcca gagaagcgtc gtcgtcgtaa tacagcttcg ccatggtgtt
      961 gggtcctttc gggtagacac agagacccca gacgccgtga cgtcgatccg tgggggcctc
     1021 cggcagcatc ggtgctggcg cgttgtcgcg cccctagtct agcacgaggc cgccgtcggc
     1081 gcgaaggcag ccggcaacgt cgggaccccg gacgtctgca ctggccgagc gcgtacggcg
     1141 aggctccgcg ccgcgcaggc cccggaaggc cgacggcgaa acgccgcact gccgcggcat
     1201 gcggcgcaag cgctcccatg ttcctggagg cggtacccat gctccagagc tccctggccc
     1261 tactcggcgg atccaaagcc gtccagtcgg acccaggcga cctcttcgcc tggcccatca
     1321 tcacccaaga ggacgaggat gcgtgtctcg aggtgctccg tcgtggtgcc atgtctgaca
     1381 ccgacgtgac ccgcgagttc gagcgggagt tcgccgcgtg gcacggcatc gagtacgccc
     1441 tggctcactc gagcggcacc gccgcgctcg aggcagccat gtgggcctcc ggcgtccgtg
     1501 ccggcgacga aatcatctgt ccgagcctga cctactgggc ctccgcacta cccgcgttct
     1561 gcctgggagc caccgtcgtg ttcgccgaag tccaggccca cagcatgtgc ctcgacccca
     1621 acgacatcga gcatcgcatc accgaccgca cccgcgccat cgtcgcggtg cactactgcg
     1681 ggcatccctg cgacatggat cccatcatga gcattgccga acgacacggt gtcaaggtca
     1741 tcgaggacgt gtcccatgcc cacggcggcg aataccatgg tcggctgctc ggaaccatcg
     1801 gtcactgcaa cgccatgtcc accatgtcgg gcaaggccct gcctgtgggc gaaggaggct
     1861 tcctgctgac ccgcgaccgg ctcatgtacg agcgtgccgt cgcctgggga cactacgagc
     1921 ggacggccga ggtgcttcga gatccttcac tcgctccata cacgggcctt ccgtggggtg
     1981 cacacaagta ccgcatgcac cagctatcat ccgccgtcgg acgggtccag ctccgccact
     2041 accgctcgcg catggaagag atccgccggt caatggacca cttctgggac ctgctggagg
     2101 gtgtacccgg cgtccgcgcc caccggccac ccaaggggag tggctcgcac aatggcggct
     2161 ggtatgcccc tcacggaatg tacgtgcccg aggagctcgg agggctgccc gtggggcgct
     2221 tctgcgaggc cgtgcaggcc gagggcgcgc ccacccatcc tggtgccaac ctgctgctcc
     2281 atcttcatcc attactcaat gatgcggata tctacggcca cggtaagccc acccggattg
     2341 cccatgcggg gcgcgacgtg cgccagccgt cgggtagcct cccggtcacc gagtcgatac
     2401 ccgagcgcac ctatgccgtc ccatggttca agcacttccg gcccagcgtg atcgccgagt
     2461 atgccctggc tttccgcaag gtggcggagc atgccgatga tctccttgga gcacccgagc
     2521 gcccgggtga tcccgaatgc cggggctgac gcgaagggag cggagactgg tggccccggg
     2581 ccgtaacgac gacgcgcggg aatgcgaacc ttcagggaac tcccggcccc ccctggggtc
     2641 ctattgatag cgaagaacga cagccgttcg gtcttggcct gcttggaatc accgccgttt
     2701 gtggaagcct agtggtatgg gatggcctcg cctgccgagg ccggagccag agcaggggct
     2761 tcggagcgca gaggcgggcg tgacctgggg ccggcgcggg aaagcacggg gctgtggatt
     2821 ggcggtcatg gggtgggagg tggatgcggt gccgaggcaa cacgcatccc gtacggtggt
     2881 ggctgagtcc gtcaaaacgc tgggggcgtc cataacgccg gttcccgcag ctcaccgagc
     2941 agcaggttgc ggtcaccggt cgtcccccag gcgtctggag ttgcgtgcgc ggcgcggcaa
     3001 gggtctgctg gctgccgatg aggtgaggct gtcccgacgg atccgtcgtg gcgcctgcag
     3061 cgtccggtac gaccccgaca cctgtacgat ccacctgatc ccgtccgccc cgttgcgtgc
     3121 cgacacgctg tacgccgtct ggcgggccga tctgaccggg attccctccg ggccaggcgc
     3181 cgaggggctc cccgtcgagc ctttcatact cgagttccgg accgctggca acgcgccgcg
     3241 ccgccccaac gcgccgctgc c
//
LOCUS       DAOSPL010000384         3260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29523_length_3260_cov_2.713573, whole genome shotgun sequence.
ACCESSION   DAOSPL010000384 DAOSPL010000000
VERSION     DAOSPL010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29523_length_3260_cov_2.713573"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3260)
                     /locus_tag="PLQ54_08205"
     CDS             complement(<1..>3260)
                     /locus_tag="PLQ54_08205"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOS93279.1"
                     /translation="TESDQGGDEDDRGSSIFLQGFSFGWSLWRIGEGNQSDNYGVSRW
                     PTGYRVVHVQDMDATTAQPDNDGFTVFSTVVDTGSPTSTGRPWERQDYYLGIYVEADA
                     DADGHGAADFLFDNLTGFTLRPWSRQYDILVVHDFTCGQHCKYWLAPVRGISNPVEES
                     SYANVGMPVESYFGFPGTGRYGSRNRSDPPGQVGEGSTHTITGLNTMGIPDWNTWEPG
                     YDKWRIASRGRILPGVMAEYLPANHYPGDEGYGSVDPEYPIWAVSVDAQGFATAPNRT
                     GTNPDPPLSNLYVSDRCAFWFAPYTGNLLLGKESGTISDAQTQADLTSFVNQGGRILV
                     SGQDIGWALTLNGSIGTPAFYTNVLGATFVRDDVGGNSNNSLNNDGASLAAAGSGFAN
                     NPVVYGPRANWHGDNRVCGDDQPTSLYFGWTDTSSNATWHREGAMQQAWPDVITAAGG
                     ASPVYTYTPGGTAGVYRTGFPRGGRTVYLSFGLEAVHRGFYYTERCGIRSDNFRHKIL
                     SNTSNWMRTASIRGLVTAGPASADYPPGMPIANALVVARSLSGGLSYGTFTTAKGYYQ
                     FRGLPVDQYAMTAQIRGHLGDHQAGVTTWGKGFHPQEEPAPGWVPYDETFVAREDFNL
                     LPAPPGAIDGYVYSSIDLDGDTTNDPDPVANIKVRATSRTGDPAWVGEAITDAKGYYL
                     IRNVPVDTYDVVANPDEELNFSSDTQEDITVQSGQTVRVDFTLDPAPGNLQGTVTDQA
                     TTDPVQGAEVEIQGIVRPDGSAYSATTGADGRYRIQDIPAGVRTVIAGAPGYLESTGQ
                     ATIPRNGTATLNFALEAVPDGTVTGRVTDSTGTNGIAGITVQGLFASVVKGETTTDAQ
                     GYYELSLPAARYTVKAVHPTGDYAFSPEAGTAVTVVSNETVSGINFQAVAQTSLRPGL
                     HIIGPAYDYLDKAPPAVFGREAATFKMARWVPAAGAYSVYSPGTADPAVSRFLRGRGY
                     FVSFTTAAEVLAVGTPTDSQARRAPVEVPLSAASGGWNLIGNPYDWDIDFSLSTVRVG
                     GQVISMQEAAAQGVISPVLFAYDNGYVETSVLREWRGGWVQARQDVGLILSSEPVL"
BASE COUNT          516 a         1071 c         1073 g          600 t
ORIGIN      
        1 cagcacgggc tcgctcgaca ggatcaaacc cacgtcctgc cgggcctgca cccatccgcc
       61 acgccactcg cgcaaaacac tcgtctccac gtacccgttg tcataggcga acaggacggg
      121 cgagatcacg ccttgggcgg cggcttcctg catgctgatc acttggccgc cgacccggac
      181 cgtggacaag ctgaagtcga tgtcccagtc gtaaggattg ccgatcaggt tccatccccc
      241 gctggccgcg ctcaggggta cttcgaccgg agcccggcgc gcctggctgt ccgtcggcgt
      301 tccgacggcc agtacctcgg cggccgtcgt gaaactcacg aagtagccac gtccacgcag
      361 aaaccggctc acggccgggt cagccgtccc cggcgagtag acggagtacg caccggcagc
      421 cggtacccag cgagccatct tgaacgttgc cgcctcccgc ccgaacactg cgggtggcgc
      481 cttgtcgagg tagtcgtagg ccgggccgat gatgtgcagt ccggggcgaa gcgaagtctg
      541 ggccacggcc tggaagttga tgcccgagac ggtctcgttg gagacgacgg tcaccgcggt
      601 gccggcctcc gggctgaatg catagtcacc cgtcggatga acagctttga ccgtgtaccg
      661 cgcggcggga aggctgagtt cgtagtagcc ttgtgcgtcc gtggtggtct cacccttgac
      721 cacactggcg aacagcccct gcaccgtgat ccccgcgata ccgttggtcc ccgtcgagtc
      781 ggtgactcgt cccgtgaccg tgccatctgg caccgcttcc agcgcgaagt tgagcgtggc
      841 cgtaccgttt ctcggtatgg tcgcttggcc cgtgctctcg aggtagcccg gggcccccgc
      901 tatcaccgtg cgcaccccag ccgggatgtc ctggatgcgg tagcggccat cggcaccggt
      961 ggtggccgaa tacgccgagc cgtcgggtcg aacgatgccc tggatctcca cctccgcgcc
     1021 ctggacggga tcggtggttg cctggtcggt cacggttccc tggaggttgc cgggtgccgg
     1081 atccagcgtg aagtccaccc gcaccgtctg gcccgactgg accgttatgt cttcctgggt
     1141 gtcgctgctg aagttcagct cctcgtccgg gttggccacc acatcatagg tgtccaccgg
     1201 cacgttgcgg atcaggtagt accccttggc gtccgtgatg gcctccccga cccaggctgg
     1261 atcgccggtt cggctggtgg cacggacctt gatgttggcc actgggtccg ggtcattcgt
     1321 cgtgtcgcca tcgaggtcga tgctggagta gacgtatcca tcgatggctc cgggcggcgc
     1381 cggtagaagg ttgaagtcct cgcgcgccac gaaggtctcg tcatagggca cccagcccgg
     1441 tgccggttcc tcctgcggat ggaagccctt gccccaggtc gtgacgccgg cctggtggtc
     1501 gccgagatgc ccacggatct gcgcggtcat cgcatactgg tccacgggga gtccacggaa
     1561 ctggtagtag cccttcgcgg tggtgaacgt gccgtaggag agcccgccgc tgagcgatcg
     1621 ggccaccacc agtgcattcg cgatcggcat gccgggtggg taatccgccg aagccggacc
     1681 cgcggtcacc aagccgcgga tgctcgcggt ccgcatccag ttggatgtgt tactcaggat
     1741 cttgtgacgg aagttgtcgg accgaatacc gcacctctcg gtgtagtaga agccgcggtg
     1801 cacggcctcg agaccgaagg acaggtagac tgtgcggccc cctcgcggaa accctgtgcg
     1861 gtagacgccg gcggtgccgc caggcgtgta ggtgtagacg gggctcgcgc cacctgcagc
     1921 cgtgatcaca tccggccacg cctgctgcat cgcgccctcg cgatgccacg tggcgttgct
     1981 gctggtgtcc gtccagccga agtacaggct ggtgggctgg tcatcaccac acactctgtt
     2041 gtctccatgc cagttggcgc gcgggccgta gacgacgggg ttgttggcga agccgctccc
     2101 ggcggccgcc agggacgcgc catcgttgtt caagctgttg ttgctgttcc cgcccacgtc
     2161 gtcgcggacg aacgtggccc caagaacatt ggtgtagaac gccggggtcc cgatcgaccc
     2221 gttgagggtc aacgcccacc cgatatcctg gccggagacc aggatgcgcc cgccctggtt
     2281 cacgaagctg gtgagatcgg cctgggtctg cgcgtctgag atggtgccac tctctttgcc
     2341 caacagcagg ttgccggtgt agggcgcaaa ccagaaggcg caccgatcgg agacgtagag
     2401 gttgctcagt ggcggatccg ggttggttcc cgtacggttg ggagcggtag cgaagccctg
     2461 cgcatcgacc gagaccgccc agatcgggta ctccgggtcc acggagccat agccctcgtc
     2521 tcccggatag tggttggcag gaaggtactc ggccatcaca ccgggcagga tgcgcccgcg
     2581 gctcgcgatg cgccacttgt cataccccgg ttcccaggtg ttccagtcgg ggatgcccat
     2641 ggtgttgagg ccggtgatgg tgtgcgtcga tccctcaccg acctggccgg gcgggtcgct
     2701 tcggttccgg ctgccgtacc gtccggttcc ggggaatccg aagtacgact cgaccggcat
     2761 acccacattc gcgtaggagg actcctccac agggttcgag atcccgcgga cgggcgccag
     2821 ccagtacttg cagtgctggc cgcacgtgaa gtcatgcacg accaggatgt cgtactggcg
     2881 actccatggc ctcagcgtga accccgtgag gttgtcgaaa aggaagtcgg cggcaccatg
     2941 cccatcggcg tcggcgtctg cctcaacgta gatgcccagg tagtaatcct gacgctccca
     3001 aggcctcccc gtgctcgtcg gtgacccggt gtccaccacg gtggagaaga ccgtgaaccc
     3061 atcattgtcg ggctgcgcgg tggtcgcgtc catgtcctgc acgtggacaa cccggtaccc
     3121 ggtgggccag cggctcacgc cgtagttgtc cgactggttc ccctcgccaa ttcgccacag
     3181 actccagccg aaggagaagc cttgcaggaa gatgctgctt ccgcggtcgt cctcatcgcc
     3241 gccctggtcc gactccgtgg
//
LOCUS       DAOSPL010000385         3253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29597_length_3253_cov_2.954034, whole genome shotgun sequence.
ACCESSION   DAOSPL010000385 DAOSPL010000000
VERSION     DAOSPL010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29597_length_3253_cov_2.954034"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PLQ54_08210"
     CDS             <1..606
                     /locus_tag="PLQ54_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOS93280.1"
                     /translation="PFATGWPRAVREVVSERYLANIHGTFYEVPREPGPDGHEPDFER
                     MKPVASHGKVITDFCTWRGLLVLSGVRRHAAGDGQCFSGPDGSGLWFGAIDDLWKLGK
                     PVGRGGPWFHTSVEADQASDPYLMANYDEMRVELSHDAPTAVSFAIEVNFDHMGFREY
                     GQVSVPPSETQVHRFPRGFRAHWVRFRVGAACRATAMLIYE"
     gene            complement(608..1405)
                     /locus_tag="PLQ54_08215"
     CDS             complement(608..1405)
                     /locus_tag="PLQ54_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS93281.1"
                     /translation="MMRIARGVSAFVLCSAVVASGLWAQDEAEEGWVSLFNGQDLEGW
                     TPKFAGSDLGVNYRNTFRVEDGLLRVAYDEWDRFSGEFGHLFYKEPFSHYRLRIEYRF
                     VGEQVTEGPGWALRNSGVMLHCQPPETMGIDQSFPVSVEVQFLGGDGTNERHTANLCT
                     PGTNVVMGGELITRHCTDSASKTYHGDQWVTVEIEVDGGTTIRHLLDGQVVLEYDQPQ
                     LDPNDGDAKPLIEAAKGELALTGGYIALQAESHPVEFRRVEILNLEK"
     gene            1579..3183
                     /locus_tag="PLQ54_08220"
     CDS             1579..3183
                     /locus_tag="PLQ54_08220"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOS93282.1"
                     /translation="MVRWTGPCCVRFAAVAAAMVLVGAPVAADLLGDLARPLDGRSMR
                     STSTQRNEDGSYAHGNWDNSNVAPGATKVVLDAKGPGMVTHMWFTFLGPEKQAWAPEG
                     SANHQELLLRVFYDGSDRPGVEAPFGDFFANSFGKRVPVISIPVVVEDADSYNCYWRM
                     PFRRSIRAEIVNESTKPLSLLYYNIDWIQLKRLDRDTPYFYAQYRQEYPVEPDKDYVV
                     LDTRGRGHYVGTVLSVRTRSPSWFGEGDEKVYIDGEPTASIWGTGTEDYFLSAWGLQT
                     TSTPYFGTPYFDQWGIVGGHTSAYRWHLNDPFVFQKSIRVTLEHWGWISPDENAKGET
                     HSWNERQDDYSSVAFWYQTGTPTFESRAPHARERALPNLDIVVPARDFADAAHHGIGD
                     AVVQENLGFFPQGQLLYHPDAREGAWVEIPFAVTVKEPRRLLIRGTRADDYGTYQPYL
                     NGVKVGGPIDLYHPSVSEWEWHLLDFWPDPGQYTLRLECVGKNPASRGYLLGIEAVRL
                     RERRPRVKEWARDAARDWRTEPILYR"
BASE COUNT          539 a         1045 c         1097 g          572 t
ORIGIN      
        1 cccttcgcga ccggatggcc gcgggcagtg cgtgaggtgg tctcggagcg gtacctcgcc
       61 aacatccacg gtaccttcta cgaggtgccg cgcgaacccg ggcccgatgg gcacgagccc
      121 gacttcgagc ggatgaagcc cgtggccagc cacggcaagg tcatcacgga cttctgcacg
      181 tggcggggct tgctggtgct cagtggcgtc cggcggcatg ctgcgggcga cggacagtgc
      241 ttctcgggcc cggacggctc gggcctgtgg ttcggggcca ttgacgacct gtggaagctc
      301 ggaaaacccg tgggacgcgg cggaccgtgg tttcacacgt ccgtcgaagc ggaccaggca
      361 tccgatccgt acctcatggc gaactacgac gaaatgcgtg tcgagctatc gcatgatgcc
      421 cctacggcgg ttagcttcgc catcgaagtg aacttcgacc acatgggctt ccgcgagtac
      481 ggacaagtgt ccgtgccgcc gagtgagacg caggtgcacc gtttcccgcg cgggttccgg
      541 gcgcactggg tgaggttccg cgtcggcgca gcgtgccggg cgaccgcgat gctgatctat
      601 gagtgattta cttctccagg ttcaggatct ccacccggcg gaactcgacg ggatggctct
      661 cggcctgtag cgcgatgtag cccccggtga gagcgagctc gcctttggca gcctcgatca
      721 gcggcttggc gtctccatcg ttcgggtcaa gctgcggctg gtcgtactcg agcaccactt
      781 ggccgtccag gagatgccgg atggtcgtgc cgccgtcgac ctcgatctcg acggtgaccc
      841 actggtcgcc gtggtaggtc ttcgaggcgg agtcggtgca gtggcgggtg atgagctcgc
      901 cgcccatgac cacatttgtc ccgggagtgc agaggttggc cgtgtggcgc tcgttggtgc
      961 cgtcgccccc caggaactgg acttcaacgg acacggggaa gctctggtcg atgcccatgg
     1021 tctcgggcgg ctggcagtgg agcatgacgc cgctgttcct gagggcccac cccggccctt
     1081 cggttacctg ctcgccgacg aaccggtact cgatccggag ccggtagtgc gagaagggtt
     1141 ccttgtagaa gaggtggccg aactcgcccg agaagcggtc ccactcgtcg tacgcgacgc
     1201 gcagaagacc atcctcgacg cggaaggtgt tccggtagtt cacgccgagg tcggatccgg
     1261 cgaacttggg cgtccagccc tccaggtcct gcccgttgaa gaggctgacc cagccttcct
     1321 cggcctcatc ctgcgcccat agccccgagg ccactacagc cgagcacagc acaaacgccg
     1381 acacgcctcg cgcgatccgc atcatgcacg ctcctcctcg ttcccgaccc atgaccagtt
     1441 caccgcggcg tgtcgtcgtc ctcccccgcc gggagggaca gcccgggctc gttaggaacg
     1501 atcgagcagc cgcccgtcac tggcggtgta gctcgggtcc acaagaccgt gacagcgggt
     1561 gatcgcgtgc cgaatgccat ggtacgatgg accgggccct gctgcgtccg gtttgctgcc
     1621 gtcgcggcgg ccatggtcct ggtcggggcc ccagtcgccg ccgacctcct tggggatctg
     1681 gcccggccgc tggatggccg cagcatgcgc tcaacgtcga cacagcggaa cgaggatgga
     1741 tcctacgccc acgggaactg ggacaactcg aacgtcgccc ccggcgccac gaaggtcgtg
     1801 ctcgatgcca aggggccggg catggttacg cacatgtggt ttacctttct cgggccggag
     1861 aagcaggcgt gggcgccgga gggttcggcg aaccatcagg agctgctgct gcgcgtcttc
     1921 tacgacggtt ccgaccggcc gggtgtcgag gccccgttcg gcgatttctt cgccaactcg
     1981 ttcgggaagc gcgtcccagt catcagcatc cccgtggtgg tggaggatgc agattcctac
     2041 aactgctact ggcggatgcc gtttcggaga tcgattcgag cggagatcgt gaacgagagc
     2101 acgaagcctc tgagcctgct ctactacaac atcgactgga tccagctcaa gaggctcgac
     2161 cgggacaccc cttacttcta cgcccagtac cgccaggagt accccgtcga gccggacaag
     2221 gattacgtcg tgctggatac caggggcaga ggccactacg tcggtacggt gctgtcggta
     2281 cgaacgcgga gcccatcatg gttcggtgag ggcgacgaga aggtctacat cgacggtgag
     2341 ccgacggcgt ccatctgggg cacgggtacc gaggattact tcttgtccgc ctggggtttg
     2401 cagacgacca gcacgcccta cttcgggacg ccctacttcg atcagtgggg catcgtcggc
     2461 gggcacacca gcgcctatcg gtggcacctg aacgacccct ttgtgttcca gaagtccatc
     2521 cgagttacgc tagagcactg gggttggatc tcgcccgacg agaacgccaa gggcgagact
     2581 catagctgga atgagcggca ggatgactac tcgagcgtgg cgttctggta tcagacgggc
     2641 acaccgacct tcgagtctcg ggcgccccac gcccgggagc gcgccctgcc gaatctggac
     2701 atcgtggtac cggctcgcga cttcgcggac gctgcccacc acggcatcgg tgacgccgtg
     2761 gtgcaggaga acctcggctt tttcccgcag gggcagctcc tgtaccaccc ggacgctcgg
     2821 gagggcgcgt gggtggagat cccgttcgcc gtgacggtga aggagccgcg gcggctgctg
     2881 atccgcggca cgagggccga tgactatggc acataccagc cctacctgaa cggggtgaag
     2941 gtgggcggac ccatcgacct ctaccacccg tcggtgtccg agtgggagtg gcatctgctg
     3001 gacttctggc ccgacccggg gcagtacacg ctgcggcttg aatgcgtggg caagaacccg
     3061 gcgtcgcgcg ggtacctgct cggcattgag gccgtgcggc tccgcgaacg ccgcccccgc
     3121 gtgaaggagt gggcccgcga cgccgcccga gattggcgga ccgagccgat cctgtaccga
     3181 taggcatccg actcggtcta ccgcgtgagg gtcaccttct tcagcccgcg gggatggtcg
     3241 ggatcgaggc cct
//
LOCUS       DAOSPL010000386         3249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29656_length_3249_cov_2.974953, whole genome shotgun sequence.
ACCESSION   DAOSPL010000386 DAOSPL010000000
VERSION     DAOSPL010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29656_length_3249_cov_2.974953"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3249)
                     /locus_tag="PLQ54_08225"
     CDS             complement(<1..>3249)
                     /locus_tag="PLQ54_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93283.1"
                     /translation="QWVPLSGEWKVLDQHLTCEAAGSSVCVLWAPERDAQSITGTVRI
                     DRRIDKGTWAFAGIGIRLDEANYWRLELVEGPDGRRYADMVESSNGQWQAQASGPGKL
                     TEAESAEMPPWEYNRDYTFRFTVDPDRVAGTIEPATGGGGGFRKTFLVPAGVSAVTAG
                     RPALYASGMAAAFSAMNVTADAQLEREVRYGSTRIGVLCGMGSEAVDTLAQRIADSLS
                     RPRWSPRMLTASELADPGVLRPGTIDILVLPDARTFPAPAVDNLYRFLRRRGKLIAVG
                     LPALSNLVWPDDGRWVSRAEALAQAPETHPIFELTPVTIGEWVRGAADMSRPASRELD
                     AAPPGVAGGSIHVRADLRGWDVLIHDTRDGALDGESPLTSFWAKGSPETPRLLIEWQE
                     RDGSRWTTRVELTTEWKRYVLAPEDLEYWPDNPSSGRGGATDHLVPENVARFSVGLAA
                     GHGGAREGAQEYWIAGLAAAKRPPGLKMPEIRPLDGLTPWYKCYPLGPVSRFAVPSAT
                     GWPQQSWIRDLGLAPGEAVASRPRMQGFGGAAGRPVRFVPLVVAEDGRGRSRGSVVSA
                     YVGVDSTYGGAVWAAVGLEPFEAAHSAGVATLVAALAKRLASGLFVLNAGTEHFSYFV
                     GEEIGLVARLARFDRALAPGYGHYDNVALRMEIHDGRTGAKLHGKIWFRGLRPFVIER
                     FEDRTGLRDLKPGLYTVTAELVDRNTVVDRITHEFSVVEDPADTPDSEIVRVRDGEFV
                     IDGRRWHPHGVNYWPSYVTGIEAPDWVRWLSPAQYDPATVDRDLEMLESLGVNSVSIT
                     FSPPDDVRSLHDFLARCHRHRIRVSLFTPHTHPLAFEPEAARQALVESKLYRHPDIWT
                     YDIAWEPHVGRYADRCRLDPLWQQWLIEQYGAVELAEADWGFAVPRTSEGGVTGPSDN
                     QITNDGDWARMVAAYRRFLDDTISRGYGRVCRLIRSIDPSHLIGARSGYGGTGGAGVD
                     VQMPFDLIAGAAHLDYISPEAYNLSGDWSTFQSGGFTTAYARWAGNGKPVFWAEYGYT
                     VFADGISRDLDEQGRHYQNMLRMVLESGANGHAAWWYPGGFRVGENSDYGII"
BASE COUNT          518 a         1120 c         1120 g          491 t
ORIGIN      
        1 cgatgatgcc gtagtcgctg ttctcgccga cgcggaaccc gccggggtac caccaggcgg
       61 catggccatt ggcgccggac tcaagcacca tgcggagcat gttctggtag tgccgcccct
      121 gctcgtccag gtcgcgcgag ataccgtcgg cgaagaccgt gtagccgtac tcggcccaga
      181 acacgggctt gccgttaccg gcccagcggg cataggcagt agtgaaccca cccgactgaa
      241 aagtgctcca gtcaccactg aggttgtagg cctccggcga gatgtagtcg agatgggccg
      301 caccagcaat gaggtcgaag ggcatctgga cgtcgacacc ggcgccgcca gtaccgccgt
      361 agcccgaccg ggcgccgatg aggtgtgagg ggtcgatgga tcggatcagc cggcacacgc
      421 gcccgtagcc gcggctgatg gtgtcgtcca ggaaccgccg gtaggcggcg accatgcgtg
      481 cccagtcgcc gtcgttggtg atctggttgt ctgacggccc ggtgacaccg ccttcggacg
      541 tccgagggac tgcgaagccc cagtcggcct cagccaactc aacagcgccg tactgctcga
      601 tgagccattg ctgccacagc ggatccagcc gacagcggtc agcatagcgg ccgacatggg
      661 gctcccacgc gatgtcatag gtccagatgt ctggatgtct atacaacttc gactcgacca
      721 gcgcttgccg tgcggcttcg ggctcgaacg ccagcgggtg tgtgtgggga gtgaacaggc
      781 tcacgcggat ccggtgccgg tggcaccggg cgaggaagtc atgcagcgag cgcacatcgt
      841 caggcgggga gaatgtaatg ctcactgagt tgacgcccag gctctccagc atctcgaggt
      901 cgcggtcgac ggtggctggg tcgtactggg cgggcgaaag ccagcggacc cagtctgggg
      961 cctcgatgcc cgtgacgtac gagggccagt agttgacgcc gtgcggatgc caccggcgtc
     1021 cgtcgatgac gaactcgcca tcgcggacac gcacgatctc gctgtctggt gtgtccgccg
     1081 ggtcctcaac aaccgagaac tcatgggtga tgcggtcgac cacggtgttc cggtccacca
     1141 gttctgccgt gacggtgtac aggcccggct tgaggtccct caagcctgtc cgatcctcga
     1201 agcgctcaat cacgaagggg cgcaggccgc ggaaccatat cttgccatgg agcttggctc
     1261 cggttcgccc gtcgtggatc tccatccgta gggccacgtt gtcgtagtgg ccatagccgg
     1321 gggccagggc ccggtcgaac cgggccagcc tggcgactag gccgatctcc tcgccgacga
     1381 agtagctgaa gtgctcggta cccgcattga ggacaaataa cccgctggcg agccgcttcg
     1441 cgagagccgc caccagtgta gcgacgcccg ctgaatgggc cgcctcgaac ggctcaagcc
     1501 ccacggccgc ccacacggcc ccgccatatg tggagtcgac gcccacatag gccgacacca
     1561 cggatcctcg cgagcggccc cggccgtcct cggcgaccac cagaggcacg aaccgcacgg
     1621 gccggcccgc ggcgccaccg aagccctgca tgcgcgggcg gctggccacg gcttcgccag
     1681 gcgccaggcc aaggtccctg atccagctct gctgcggcca accggtagcc gacggcacgg
     1741 cgaacctgct gaccggtccc agcgggtagc acttgtacca gggcgtcagc ccgtccagcg
     1801 gccggatctc gggcatcttc agcccgggtg ggcgcttggc ggcggccaag cccgcgatcc
     1861 agtactcctg cgccccctcg cgcgctccgc cgtgtcccgc cgcgaggccc accgagaacc
     1921 gggccacgtt ctccggcacc aagtggtcgg tggcaccgcc acgaccggag ctcgggttgt
     1981 cgggccagta ctcgagatcc tcgggggcga gcacgtagcg tttccactcg gtggtgagct
     2041 caacacgcgt ggtccaccgc gagccatcgc gctcctgcca ctcgatgagc aaccgcggtg
     2101 tctccggact tcccttagcc cagaagctgg tgagcgggct ctcgccgtcc agcgcgccgt
     2161 ctcgcgtgtc gtggatgagc acatcccagc ccctcaggtc ggctcggaca tggatcgagc
     2221 cgcctgcgac cccgggcggg gcggcgtcaa gctcgcggct ggccggccgc gacatgtcgg
     2281 cggcgccccg gacccactcc ccgatcgtga ctggggtcag ctcgaagatg gggtgcgtct
     2341 cgggggcttg cgccagggcc tcggcccggc tcacccaccg cccatcgtcc ggccacacga
     2401 ggttcgacag tgcaggcaga ccgacggcga tgagcttccc gcgccgccgc aggaagcggt
     2461 acaggttgtc gacggccggt gccgggaagg tgcgggcatc agggaggacc aggatgtcga
     2521 tggtgcccgg ccgcaggacg ccggggtccg ccagctcgga cgccgtcagc atccgagggg
     2581 accagcgtgg gcgcgacagg ctgtcggcga tccgctgggc gagggtatcc accgcctccg
     2641 accccatgcc gcagaggacg ccgatgcggg ttgagccgta gcgcacctcc cgctcgagct
     2701 gggcatcggc cgtcacattc atcgcggaga aggccgccgc catgcccgag gcgtagagcg
     2761 ccggccggcc ggctgtgacg gcgctgacgc cggcgggcac caggaaggtc ttccggaagc
     2821 cccctccccc accggtggcg ggctcgatcg tgccggcgac gcgatcgggg tccacggtga
     2881 agcggaatgt gtagtcccga ttgtactccc aaggcggcat ctcggccgac tcggcctcgg
     2941 tcagcttgcc ggggcccgag gcctgggcct gccactggcc gttgctgctc tcgaccatgt
     3001 cggcgtagcg gcggccatcg gggccctcga cgagctcgag gcgccagtag ttggcctcgt
     3061 cgaggcggat gccgatgccg gcgaaggccc aggtgccctt gtcgatgcgc cggtcgattc
     3121 gtaccgtgcc ggtgatggac tgcgcgtcgc gctccggggc ccacagcacg cagaccgacg
     3181 acccagctgc ctcgcaggtg aggtgctggt cgaggacctt ccactcgcca gagagcggca
     3241 cccactggc
//
LOCUS       DAOSPL010000387         3249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29657_length_3249_cov_2.861616, whole genome shotgun sequence.
ACCESSION   DAOSPL010000387 DAOSPL010000000
VERSION     DAOSPL010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29657_length_3249_cov_2.861616"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..1383
                     /locus_tag="PLQ54_08230"
     CDS             232..1383
                     /locus_tag="PLQ54_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93284.1"
                     /translation="MKYLIGSGLLLCVALPHPARADLVLLDEYWSPEIAGNDVVVAEV
                     DTQETGDPTEAKFGECSAKLWNESGSPNVRFRGAAPMTLSQIPPGDCELSLWYRTNGW
                     TGPWRVEVWVWHEALHNAPAPAPVRVLQTTLDGGGPDGQLLADDQWHEARGRLTAGES
                     YEATPKEEHMGAAYVWLVPVGGWNVAHRTYIDRISIEVVDGDRKGEPAPAPARRIRPK
                     PGAQTDGPGWIWWEGEDAFEHNVPTGGATVPMTEAQQALLSGNAWLSHHGVEDLQVQW
                     EITVPEDGRYTLWCRGQGAPFRWCWDDGTWSECGFEAVWTDHVPYQDHGAYGWLRLCW
                     VCLGEVQMSRGRHVLAVGGVPDDQAVGFDCWLVTRGGFMPDGARRPADE"
     gene            1489..1860
                     /locus_tag="PLQ54_08235"
     CDS             1489..1860
                     /locus_tag="PLQ54_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93285.1"
                     /translation="MPLFRRLPLSFMPVAGLPVIGLGTYLLVSERSEATTDELDGLLA
                     LSQRLADRIDVHVSANQGLVRHLAATDHVVGFLAKRPRSEADRDTFDRWLALQSALSS
                     EPIDIYAMDRTGPCVASTNRS"
     gene            1903..2445
                     /locus_tag="PLQ54_08240"
     CDS             1903..2445
                     /locus_tag="PLQ54_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93286.1"
                     /translation="MGGDYFQTDWLVGSLTRVPGVFSASPVVRGETITGVLAVKLRCT
                     HIRKAVTELGTQGRNAFLINHQGIVLVHSQPSHEYTSLVELSEDEQDSLRTSRQFMQE
                     RIRQYPVGPELTQALNNVLQSGRPQTVAYSLGCVPHWYGAWTRTGWRAFRLRTRPSTP
                     PPGEFRGTPWCSPRSHCSCV"
     gene            2409..2930
                     /locus_tag="PLQ54_08245"
     CDS             2409..2930
                     /locus_tag="PLQ54_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOS93287.1"
                     /translation="MVFAALALLVCLAASLVMSHRLSRPIARLTEAVEQFGAGDSDAR
                     AARVDGGEVGLLAARSNEMADAISSNTEVLERRVRERTHELTRASEESARTIDELQKA
                     LAPVRTLGGLLPICSSCTRIRNDEGYWTRIEAYIRDHSEAEFTHGICPECAKQLYPEA
                     APRADRTPGMKDG"
BASE COUNT          522 a         1031 c         1140 g          556 t
ORIGIN      
        1 ggaccgcggc aggtcgcaag gcacggagac acggtgcggt gggtggtgcc ccccgcgagt
       61 gccgtggtgt taaccgttgc cgcggagcgc tagaggctcc cattccgggc gcctgagagc
      121 gatacaggtt cctgggggcg cggcccaaag tcgcgggcga gcgcggcggc cttgtctgct
      181 gcccgaccat ccgtgagtgc ggagtgcttg ctccgcgggg aggggcttgc agtgaagtac
      241 ctcattgggt ctggccttct gctttgcgtc gcgttgcctc acccggcacg cgctgatctg
      301 gtcctgctgg atgagtactg gtcgcccgag atagcaggga atgatgtggt cgtggcggag
      361 gtcgacacgc aggagaccgg cgacccgacc gaggccaagt tcggcgagtg cagcgcgaag
      421 ctctggaacg agtccggttc gcccaacgtg cgcttccgcg gtgccgcacc gatgacgctc
      481 tcccagattc cgcccggtga ctgcgagctg tcgctgtggt accgcaccaa cggatggaca
      541 ggcccgtggc gcgtagaggt gtgggtctgg catgaagcac tgcacaacgc accggcccct
      601 gcaccggtga gggtgctgca aaccacgctc gatggtggcg ggccggacgg ccagcttctg
      661 gccgacgacc agtggcacga ggcccggggc agactgacgg caggcgagtc ctacgaggcc
      721 acgccgaagg aagagcacat gggcgccgcc tacgtgtggc ttgttcccgt aggtggctgg
      781 aatgtggccc atcgaacgta catcgaccgc atcagcatcg aggtggtaga cggtgaccgg
      841 aagggtgaac cagcgcctgc tcccgctcgc cggatccggc cgaagcccgg agcgcagacc
      901 gatgggcccg gctggatctg gtgggaaggc gaggacgctt tcgagcacaa cgtgcccacc
      961 ggcggcgcga cggtgccgat gaccgaggcc cagcaggctc tgctctccgg caacgcgtgg
     1021 ctcagccacc acggagtcga ggacctgcag gtgcagtggg agatcaccgt gccggaagac
     1081 ggacgctaca cgctctggtg ccgcgggcag ggcgcgccct ttcgctggtg ttgggacgat
     1141 gggacgtgga gtgagtgcgg cttcgaggcc gtctggaccg atcatgtccc gtaccaggac
     1201 catggcgcct acggatggct ccggctctgc tgggtgtgcc tgggcgaggt ccagatgtcg
     1261 cgcggccgac acgtgctcgc agtgggcggc gtgcccgatg accaagccgt ggggttcgac
     1321 tgctggctgg tcacgcgggg gggcttcatg ccggacggcg cgcggcggcc tgccgatgag
     1381 tagcccgccg cggatcggcg tgatgcgagc cgggccgggc gcggggaggg ggagggcttg
     1441 ctgagcggtt ccgaccgacg cccccgcctc tcgcgcgctg cggtgggcgt gcctctcttt
     1501 cgacgcttgc cgctgtcgtt catgccggtt gcggggcttc cggttatcgg gctcggcacc
     1561 tatttgctgg tctcggaacg gtccgaagct accaccgacg aactcgacgg gctcttggcg
     1621 ctgtcccagc ggctcgccga caggatcgac gtccacgtat cggcgaacca ggggcttgtt
     1681 cggcatctgg cggccaccga ccacgtggtt ggcttcctgg cgaaacgacc gcggtcagag
     1741 gccgaccggg atacgttcga ccgatggctc gcgcttcaga gcgctctgtc ttcggagccg
     1801 atcgacatct acgccatgga ccgcacgggc ccttgtgtcg cgtccaccaa ccggtcgtag
     1861 ctcggcagca acttcgggtt ccgcccctac ttcaaggccg cgttgggcgg cgactacttc
     1921 cagacagact ggctggtcgg ttcgctgacg cgggtgcctg gggtcttctc ggcatcacca
     1981 gtggtgcgcg gagagacgat caccggtgtc ctcgcggtaa agctcaggtg cactcacatc
     2041 cgcaaagcgg tgaccgagct gggcacccag ggacgcaacg cattcctcat caatcatcaa
     2101 ggcatcgtgt tggttcacag tcagccgtcc cacgagtaca cgtctctcgt cgagctatcg
     2161 gaggacgagc aggactcact ccgcacgagc cggcagttca tgcaagagcg catccgccag
     2221 taccccgtcg ggcccgagct gacgcaggcc ctcaacaacg tcctgcagtc cggtcggccc
     2281 cagacggtgg cctactcgct cggatgcgtg ccgcactggt acggtgcgtg gaccaggact
     2341 gggtggcggg cgtttcgtct ccggactcgg ccgtctacgc ctcctccggg cgaattccgc
     2401 gggacaccgt ggtgttcgcc gcgctcgcat tgctcgtgtg tctagcggcc agcctggtga
     2461 tgagccaccg cctgtcccgt cctatcgctc gcctgacaga agcggtcgag cagttcggag
     2521 ccggagacag cgacgcccgg gccgcacgag tcgacggggg cgaggtaggt ctcttggccg
     2581 cgcggtccaa tgagatggcc gatgccatct catcgaacac cgaggttctg gagcggcgcg
     2641 tgcgcgagcg aactcatgag ctcactcggg cgagtgagga gagcgcgcgg accatcgatg
     2701 agctacagaa ggctctggcc ccggtccgga cacttggcgg actgctaccc atctgtagtt
     2761 cgtgcacgag gatccgcaac gatgagggct actggacgcg gatcgaggcc tacattcgcg
     2821 atcactctga ggctgagttc acgcacggga tctgcccgga gtgtgcgaag cagctctatc
     2881 cggaggcggc gccgagggct gaccgcacgc cggggatgaa agacggatga atcaccgcac
     2941 ggcctccatc ggccgcgtca acacccctgc tttgcagttc cggtcggccc ctctcctggc
     3001 tgatcggctg gacagcttac actcagtaag ttacactgat ggtgccgggg ggcgatggcg
     3061 ggcggatccc gccagggaca cactactcgc cctccgcacg gaggcgaaag tgcgcagttc
     3121 agccccttcc gaagaggaac tcattctttt ccgcgcgaga ctggcatcgg tcctcgccgc
     3181 gctaggtgtt tcgcgcgccg agttgggcag acgacttggg ggcaagtcaa gccagtatgt
     3241 ctcccagct
//
LOCUS       DAOSPL010000388         3241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29740_length_3241_cov_3.605148, whole genome shotgun sequence.
ACCESSION   DAOSPL010000388 DAOSPL010000000
VERSION     DAOSPL010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29740_length_3241_cov_3.605148"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="PLQ54_08250"
     CDS             complement(<1..1221)
                     /locus_tag="PLQ54_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HOS93288.1"
                     /translation="MIWFAAMSVPLLGLAAGEPDGAAVVRAEGFRGIWFTLGQLEGPY
                     GDKYSGGLGTYTADHVPIAIHVPWVGKTFFVFGGTPGPAERHLLAMISYYDHVRWVVA
                     RPVVVHDKGGVNDPHDNPSLAIDGRGHLWVFVSGRAEKRPGFIYRSLSPYSIDGFERV
                     AEFQFAYPQPWWMGDSGFLFCLTRYTQGRELYWQTSPDGRTWAPQRKLAGFGGHYQVT
                     ATRGTQLVTAFNWHPGGDVDRRTNLYFLQTDDGGQSWRTVTGDPVATPLADVANPALV
                     RDYAAEGRLVYINDVNLDAEGRPVVSYITSGSFRPGPEGEPREWTIAHWTGGEWSYSM
                     VCTTDHNYDMGSLRIEPDAWRIIGPTDPGPQPWCTGGEMVLWESRDQGATWRRACQVT
                     HDSPRNHSYARCPVN"
     gene            1470..2075
                     /locus_tag="PLQ54_08255"
     CDS             1470..2075
                     /locus_tag="PLQ54_08255"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HOS93289.1"
                     /translation="MTAASTVPSVDDVQKFHGHMCLGLALGYRVAIEGLAELGVARSE
                     DEELVAVVENDSCAVDAIQVVLGCTFGKGNLVFRDWGRRVYTFYSRHRETGVRVSEHY
                     NGFADDPEWAALRERAAAGDESPETREALATRTEAHIRAILNGPRDAVLTVSSVPFDP
                     PAMAQIESSEPCEACGEPTMASRLVEHGGRRICRACVPAGG"
     gene            complement(2072..>3241)
                     /locus_tag="PLQ54_08260"
     CDS             complement(2072..>3241)
                     /locus_tag="PLQ54_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93290.1"
                     /translation="TPPRCSSYDGHRHRHESHRAEDRGKLMFVVLLRLLTGPLALAPT
                     DAPYPPSPVIAGVSFDWATHQRAAPGSDNWPLTWADDGHQYTAWGDGGGFGGTNSEGR
                     VSLGVARIEGDFEGHRGINLWGGRGNEERNSPTFPGKSYGILCVGGVLTMWVGMFEPD
                     ADPWRDVRLAVSRDHGFSWQLADWVLTREDGVMLPTMCQFGQDYAGARDEYVYHYWPR
                     YRSAHGPDGYSDKVPWIVVQRPGAIDLARVHRDRILDRGAYEFFAGVDDHGAPHWTRD
                     LSAREPVFEDPNGVGWCVSVSHNAGLQRYLLCTEHTETHRGKLGIFDAPEPWGPWTTV
                     AYEDAWGEGHVPVNTFYWNFANRWLSDDGRRFVMTFTGRQENDSWNALRGAFLLR"
BASE COUNT          534 a         1138 c         1053 g          516 t
ORIGIN      
        1 gttgaccggg catcgagcat acgagtggtt gcgtgggctg tcgtgcgtca cctggcacgc
       61 ccgccgccac gtcgctccct ggtcgcggct ctcccacaga accatctcgc cgccggtgca
      121 ccacggctgc gggccgggat cggtcgggcc gatgattcgc cacgcatcgg gctcgatccg
      181 tagagacccc atgtcgtagt tgtggtccgt ggtgcacacc atgctgtacg accattcgcc
      241 ccccgtccag tgggcgatgg tccactcgcg cggttcgccc tccgggccgg gccggaaact
      301 gcctgacgtg atgtaggaga cgacgggccg cccctcggcg tcgaggttca cgtcgttgat
      361 gtagaccagc cgcccttcgg ctgcgtagtc gcggaccagc gccgggttgg cgacatcggc
      421 cagtggcgtg gcaacaggat cgccggttac cgtacgccaa ctctgcccgc cgtcatccgt
      481 ctggaggaag tacaggttcg tccgcctgtc gacatcgccg ccgggatgcc agttgaacgc
      541 cgtaaccagc tgggtaccgc gagtcgcggt cacctggtag tgccccccga aaccggccag
      601 cttccgttgg ggcgcccacg ttcggccatc ggggctcgtc tgccagtaga gctcgcgacc
      661 ctgggtgtag cgcgtcaggc agaacaagaa gccggagtcc cccatccacc atggctgcgg
      721 gtaggcgaac tggaactcgg ccacgcgctc gaaaccgtcg atggagtagg gtgacaggct
      781 ccggtagatg aagccgggcc tcttctcggc ccgcccgctg acgaagaccc acagatggcc
      841 ccggccgtcg atcgccaggc tggggttgtc gtgcgggtcg ttcacgccgc ccttgtcgtg
      901 caccaccacg ggccgcgcca caacccagcg gacgtggtcg tagtaggaga tcatcgccag
      961 caggtgccgc tccgctggcc ccggggtgcc gccgaacacg aagaaagtct tcccgaccca
     1021 tggtacgtgg atcgcgatgg gcacatggtc ggccgtgtag gtgcccagac cgccgctata
     1081 cttgtcgcca taggggccct cgagctgacc gagcgtgaac cagatgcccc ggaagccctc
     1141 ggcgcgcacc actgcggccc cgtcgggctc gccggccgcg agaccgagca gcggcaccga
     1201 catcgccgcg aaccagatca tccctctacc tccgtcaaca ccccagcgcc ccaaaccacc
     1261 gtgtgtctcc cctcactgcc ggtgcctcca gtgtccaatc ggggcgacac cgtctccgat
     1321 cggaatgggt ggcatctcgc cgattgcaga cagcgatcat acctgccggg actcaccaaa
     1381 cccaaggtgc aggggcaccc gcatcttgcc acgatccctg gactcgtgct acggttccaa
     1441 ctccgcatca ggcgtagggg ggtaccgtca tgaccgcggc gtccaccgtg cccagcgtcg
     1501 atgacgttca gaagttccat gggcacatgt gcctggggct ggcgctgggc taccgggtgg
     1561 ccatcgaggg gctggccgaa ctgggagtcg ctcggtccga ggatgaggag ctcgtggctg
     1621 ttgtcgagaa cgactcgtgc gccgtcgatg ccattcaggt cgtgctcggc tgcaccttcg
     1681 gcaagggcaa tctggtgttc cgcgattggg gcaggcgggt ctacaccttc tactcccgcc
     1741 accgcgagac cggcgtccgc gtgagcgagc actacaacgg cttcgctgat gaccctgagt
     1801 gggcggccct ccgcgagcgg gctgccgctg gcgatgaatc cccggagacc cgggaggcac
     1861 tcgccacccg caccgaggca cacatacgtg ccatcctgaa tgggccgcgc gacgcggtcc
     1921 tcacggtcag ctcggtgcca ttcgacccgc cggccatggc gcagatcgag tcctcggagc
     1981 catgcgaggc ctgcggggag cccaccatgg cttctcgcct cgtggagcac ggaggccggc
     2041 ggatctgccg agcctgcgtc ccggcggggg gctaacgcag caaaaacgcc ccgcgcagcg
     2101 cgttccacga gtcgttctcc tggcgcccgg tgaaggtcat cacgaaccgc ctgccgtcat
     2161 cgctcagcca ccggttggcg aagttccagt agaaagtgtt gaccggcacg tgaccctcgc
     2221 cccaagcgtc ctcatacgcc accgtggtcc acggacccca cggctccggc gcatcgaaga
     2281 tgcccagctt accacggtgc gtttctgtat gctcggtgca caacaggtac cgctggagcc
     2341 ccgcgttgtg gctgacactc acgcaccaac cgacgccatt ggggtcctcg aagaccggct
     2401 ctcgggccga gaggtcgcgg gtccagtgcg gggcgccgtg gtcatcgacg cccgcgaaga
     2461 actcataggc cccccggtcg aggatgcggt cgcgatgcac gcgtgccagg tcgatcgccc
     2521 cgggccgctg caccacgatc cacgggacct tgtccgaata gccgtcgggg ccgtgggccg
     2581 accgatagcg gggccagtag tggtagacgt actcgtcccg cgcaccggcg taatcctgcc
     2641 cgaactggca catcgtgggc agcatcacgc cgtcctcgcg ggtgaggacc cagtccgcaa
     2701 gctgccacga gaagccgtgg tcgcgcgaca cggccaggcg gacatcacgc cagggatcgg
     2761 cgtcgggctc gaacatgccc acccacatgg tgaggacacc cccaacacag aggatcccgt
     2821 agctcttgcc cgggaatgtg ggcgagttgc gctcctcgtt cccgcggccg ccccagaggt
     2881 tgatgccgcg gtgcccctca aagtcgccct cgatgcgggc aacccccaga ctcacgcggc
     2941 cctcagagtt ggtcccacca aacccgccgc catcgcccca ggccgtgtac tgatggccgt
     3001 catcggccca ggtcagcggc caattgtcgc tgcccggggc ggcccgctgg tgggtcgccc
     3061 aatcaaacga cacacccgcg atgaccgggc tcggcggata gggcgcgtct gtcggcgcga
     3121 gggccagagg cccggtgagc aggcgtagca ggaccacgaa catgagcttc ccccgatcct
     3181 cggccctatg agactcgtgc ctgtgcctgt gtccatcata cgacgagcat cgcggtgggg
     3241 t
//
LOCUS       DAOSPL010000389         3241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29744_length_3241_cov_2.739171, whole genome shotgun sequence.
ACCESSION   DAOSPL010000389 DAOSPL010000000
VERSION     DAOSPL010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29744_length_3241_cov_2.739171"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1028
                     /locus_tag="PLQ54_08265"
     CDS             72..1028
                     /locus_tag="PLQ54_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOS93291.1"
                     /translation="MDERAREVLAIESEAIAQAADRLDASFAKARDLVLACRGRVVVT
                     GLGKSGAMARKIASTLASTGTPSFYLHSAEAPHGDFGMVTPGDVLIAISYSGATQEVL
                     DMLPYARSVGVPVIAMTGRLESPLGREADAVINCYVEREADPNNLAPTASAVVTLALG
                     DALAITVMAHRRFGPQDFARFHPGGSLGKRLARCRDIMRTGDANPLVAPETRVFDVLF
                     VMTAARSAGVATVVDADGRLVGLFTDGDLRRGLARDPGILDRTVADVMTRNPTCIGQD
                     HLAAEALRILHDHKWDNMPVVDADGRPVGIIDVQDLLDANIL"
     gene            1155..>3241
                     /locus_tag="PLQ54_08270"
     CDS             1155..>3241
                     /locus_tag="PLQ54_08270"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOS93292.1"
                     /translation="MHRIFRRCWWLQVACAVASSLGVAWCAPIGVLSGASHDMGSAAF
                     VEAALTALGQTLVLPPADPLGGKETPAPAVVVLRGYDFPKDTPGRLAEYLRRGGKVLV
                     LGPLDETVAASLGLKPVPPNSAAARPSLVAPVAGLTPGAPNRVRQNPSVVTLYEADGA
                     ERLASWLVEDGTDTGQPAAIAFANGIALGWEPQPLDGVRMGQFLISLIGRIDPDALKA
                     AADAASASVGKVGRFASMADSFAFLQNACGEHPGIRGPLDEAVARNGDALRAIQQQSW
                     AEGLAAAADAALAFQTAYATSFSSRSCEVRAVWCQTLPSAGQWGPLLQALANAGVNAL
                     FLGTSTPAMAAYPSQVVPASPRVTPGDDPLQAGLDLAPRFDVDLHAWHSVMALGDITP
                     EQVEELAKAHRIQTDPSGKRQPWLCPSHGENLELNKRIIAELLGRYPLRGIVLDYVRY
                     GGAGACYCPSCRESFAREIGAEQITDWPRCVTQGPLRQRYAEFRNAQIGRIVAALARA
                     AKEARPTAVVSVTIAPSPLDSAFGQSDWPSWLQSGDIDVIIPMNYDADAQALRTLVEG
                     QVRSADGRAEVCASIWARTPPGASPDPTRILDLAAAARLGGATGFAVRQVGPELLHEQ
                     LQLLGCGIGQLAAVPPYDGPRTRFVLLDEPVPRTHPKTYGEGDVIRATIDAGPGVPFE
                     TLRPGIENAETGEV"
BASE COUNT          507 a         1173 c         1057 g          504 t
ORIGIN      
        1 cgcatgggcc tagtaccgcg agaggagctg accgccgaac ccctcgacgg tcagaacaag
       61 tgagatctgc catggatgaa agagcacgtg aggtactcgc catcgagtcg gaggccatcg
      121 cgcaagccgc ggatcgcctg gacgcgtcat tcgccaaggc ccgcgatctg gtgctcgcct
      181 gccggggccg cgtcgtggtc acgggactcg gcaagtccgg tgccatggcc cgcaagatcg
      241 cctcgacctt ggccagcacg ggcactccgt cattctacct gcactccgcc gaggccccgc
      301 atggcgactt cggcatggtg acgcccggtg atgtgctcat cgccatctcc tactccggcg
      361 cgactcaaga ggtactcgac atgctaccct acgcccggtc ggttggcgtg cccgtgatcg
      421 ccatgaccgg ccggctcgag tcgccgttgg gccgtgaggc cgatgcggtc atcaactgct
      481 acgtcgagcg tgaggccgac ccgaacaacc tcgcgcccac ggccagcgcc gtcgtgactc
      541 tcgccctggg cgacgctctg gccattacgg tcatggccca tcgacggttc ggcccgcagg
      601 atttcgcgcg gttccacccc ggcgggagcc tgggcaaacg cctcgcccgg tgccgcgaca
      661 tcatgcgcac cggcgatgcg aaccctctgg tcgctccgga gacccgggta ttcgacgtgc
      721 tcttcgtcat gaccgcggcg cgtagcgccg gagtcgccac cgtggtcgac gccgatggcc
      781 gtctcgttgg gctgttcacc gatggagacc tccgccgagg gctcgcgcgc gaccccggaa
      841 tcctggaccg aacggtggcc gatgtgatga cccgcaaccc cacctgcatc ggccaggacc
      901 acctggccgc cgaggccctg cgaatcctgc acgaccacaa gtgggataac atgcccgtcg
      961 tggatgccga cggcaggccg gtggggatca ttgacgtcca ggatctgctc gacgccaaca
     1021 tcctctagcg acagctccct catgcggccg agcgccagcc gtgtgagacg cgtggactcg
     1081 tctcgacgtc gctagtagcg acaggggccg cgccatggcc atggctgcgg gcccccgcga
     1141 gactggggag gacggtgcac cgcatcttcc gccggtgttg gtggcttcag gtggcctgtg
     1201 ctgtggcctc gtcgctgggc gtcgcctggt gcgctcccat cggcgttctc agcggggcgt
     1261 cccatgatat gggcagcgcc gcgttcgttg aagcggcgct gacggcgctg gggcagactc
     1321 tcgtcctccc cccggccgac ccgttgggag gcaaagagac cccggcgccc gctgtcgtgg
     1381 tgctccgcgg atacgacttc cccaaagaca ccccgggtcg actcgccgag tacctcagac
     1441 ggggcgggaa ggtgctcgtg ctcggccccc tcgacgagac ggtggccgcc agtctggggc
     1501 tcaagcctgt gccccccaac tcagcggcag cccgcccctc ccttgtggcg ccggtcgcag
     1561 ggctgactcc cggtgccccc aaccgggtgc ggcagaaccc cagcgtggtg actctctacg
     1621 aagccgatgg cgctgaacga ctagcctcct ggctcgtaga ggacggcacc gataccggcc
     1681 agcccgcagc catcgcgttc gccaacggca tcgccctcgg gtgggagcct caacccctcg
     1741 atggggtgcg tatgggccag tttctgatca gcctcatcgg caggatcgac cccgatgcgc
     1801 tgaaggccgc cgccgatgca gcaagcgcct cggtgggaaa ggtcggccga ttcgcctcca
     1861 tggccgacag cttcgccttc ctgcaaaacg catgcggaga gcacccggga atccgcggcc
     1921 cgctggatga ggcggtcgcc cggaatggcg acgcactgcg cgccatccaa cagcagagct
     1981 gggccgaagg cttggccgcg gccgccgacg ccgcgctcgc attccagacc gcctatgcca
     2041 cgagcttctc atcgcgcagc tgcgaggtgc gagccgtctg gtgccaaacc ctcccgtcgg
     2101 cgggccaatg gggccccctg ctacaggccc tggccaacgc cggggtgaac gcgctcttcc
     2161 tgggcacgtc gacacccgcc atggccgcct acccgagcca ggtggtgccg gcctctcccc
     2221 gtgtcacccc cggcgatgac ccgctccagg cgggcctgga cctcgccccg cgcttcgacg
     2281 tcgaccttca cgcatggcat tcggtcatgg ccttgggcga cattaccccg gagcaggttg
     2341 aggaactcgc caaggcccac cgcatccaga ccgacccgtc cggcaaacgc cagccgtggc
     2401 tgtgcccctc gcacggcgag aacctcgagc tcaacaagcg gattatcgcg gaactcctcg
     2461 gccgctatcc tctccgtggc atcgtgctcg actacgtacg ctatggcggg gcaggcgcct
     2521 gctactgccc gtcgtgccgg gaatccttcg cgagagagat cggtgctgag cagatcaccg
     2581 attggccccg atgtgtcacg cagggcccgt tgcgccagcg ctacgccgag ttccggaacg
     2641 cacagatcgg gcgcatcgtc gccgccctgg cccgggccgc caaggaggcc agacccaccg
     2701 cagtggtctc ggtaaccatc gcgcccagcc ccctcgattc cgcctttggg cagtccgact
     2761 ggccatcctg gctgcagtcg ggcgatatcg acgtcatcat accgatgaac tacgacgccg
     2821 acgcccaggc cctccggact ctggtcgaag ggcaggtccg atctgccgat ggacgggccg
     2881 aggtgtgcgc ctccatctgg gcccgaacac cgccaggggc ctcgcccgac cccacccgga
     2941 tcctcgacct cgcggccgcg gccaggctgg gcggcgccac aggattcgcc gttcgccagg
     3001 tcggccccga gctgctccac gagcagctcc agctgctggg ctgcggcatc ggccaactgg
     3061 cagccgtgcc cccctacgat gggccgagga cacgctttgt ccttctcgat gaaccggtgc
     3121 cgcggacgca tccgaagacg tacggagagg gggacgtgat ccgcgccacg atcgacgcgg
     3181 gccccggcgt gccctttgag acactgaggc cggggattga gaacgcggag acgggtgagg
     3241 t
//
LOCUS       DAOSPL010000390         3236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29805_length_3236_cov_2.995599, whole genome shotgun sequence.
ACCESSION   DAOSPL010000390 DAOSPL010000000
VERSION     DAOSPL010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29805_length_3236_cov_2.995599"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1032
                     /locus_tag="PLQ54_08275"
     CDS             196..1032
                     /locus_tag="PLQ54_08275"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS93293.1"
                     /translation="MRIGCGTVCFRALPLDEALGRIARAGYTWVETQATAPFCPHIDP
                     WRDDPEGFRRLVAEHGFEGVTALWAPHGAILADAEAVAGISAALRWAQAAGIPVVNAG
                     DGQKPDGMSDADALAVLGERMAAILDVAASCRVYLAIEPHGSFSLTPRGLEAIMELSD
                     SPWLGINYDTANVHRATYVETAAGAYSWKPVGESADEVATLERVVGRVVHCHAKDVIG
                     ARSVALGEGEVDVTGCLRVLENHGYEGAISLETEGEEAPDQMQQLIERSRRFLEHALG
                     PR"
     gene            complement(1093..1314)
                     /locus_tag="PLQ54_08280"
     CDS             complement(1093..1314)
                     /locus_tag="PLQ54_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93294.1"
                     /translation="MREKTAVDVVCEHPPRGAEGERARHPVRLPVRPGAGFSGGIAKQ
                     SRRIGTVSGSGVREFTTAEGLDDSLLIVQ"
     gene            1450..2631
                     /locus_tag="PLQ54_08285"
     CDS             1450..2631
                     /locus_tag="PLQ54_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS93295.1"
                     /translation="MDGQGISRRTFIRTAAVAAGFAAVAGPTPARCQERTLPKRRMAG
                     TELDVSVLGYGTEFMQDRAVADHLVSFGVNHIDTAPVYQEGEAERRLVPVIRDHGDSL
                     VLTTKWGHHSDLAAPKDEFLASFAASCERMETDHVDIMYVHDARTPEKAAFASPYEAF
                     RELRDAGKIGYYGLSTHLGQEATVLKCIELGWYSVLMVAWNFFSPPSLTDALKRAADN
                     GMGIVVIKALKGLSEGQDWFQRATPEQRAVLDTFEGSMYQRAIRWTLSHDFVSSIIMS
                     VSNLDQVEENVVGGFEASRVGDARQLRRYARAVSNQVCRGCATCLGACPVGVAVPDIL
                     RYRAYHRAYGEAHTARRLYADLPESMRANACTGCGICAAHCPYGLAVPELIAEAHRLL
                     A"
     gene            complement(2674..>3236)
                     /locus_tag="PLQ54_08290"
     CDS             complement(2674..>3236)
                     /locus_tag="PLQ54_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOS93296.1"
                     /translation="RAVDEWGRLDILVCNAGILFAGGLTDIEIDRWRKVIDVNLVGYF
                     VCAREAARVMVPQQSGVIIQINSKSGKKGSFRNSAYAASKFGGIGLTQSLALELAPDG
                     IRVNSVCPGNLLDSPLWVDSLYGQYAQRWGITVEEVRAKYEQQVPMGRGCEYADVANV
                     VEFLASDDSSYMTGQAINVTGGQQMD"
BASE COUNT          551 a         1090 c         1090 g          505 t
ORIGIN      
        1 gcccaccctc ctcaaccggc gatatgctcg cctggggaat gaccaccggc caatacccag
       61 tagcggctgg aggtcatccg cccccgggtc catcacgcat ggaccgaggg agacgtagca
      121 acagaccacc gcaggcgccg ccggctgact cctgcggcac gccggggcac acgccggcaa
      181 tgggagatgg tgaccatgcg cattggctgc ggaacggtgt gcttccgggc gctgccgctt
      241 gacgaggcac tcgggcgaat cgcccgggcg gggtacacat gggtcgagac gcaggcgaca
      301 gcgcctttct gcccccacat cgacccatgg cgcgatgatc cggagggctt ccggcgcctg
      361 gtcgccgagc acggcttcga gggcgtcacg gcgctctggg cgccgcatgg cgcgatcctg
      421 gccgatgccg aggcggtagc gggtatcagc gcggcgctcc gttgggccca ggctgctggt
      481 atccctgtgg tgaacgccgg agatggccag aagccggacg gcatgtccga tgccgacgcg
      541 ttggcggtgc tgggagagcg catggcggcc atcctcgatg tggcggcaag ctgccgggtc
      601 tacctcgcga tcgagccgca cgggtcattc tcgctgacgc caagggggct cgaggccatc
      661 atggagctat cggactcgcc ctggctcggg atcaactacg acaccgcgaa cgtccaccgg
      721 gccacctatg tggagacggc cgcgggagcc tactcgtgga agccggtcgg cgagagcgcc
      781 gacgaggtcg cgacgctcga acgagtcgtc ggacgcgtgg tgcactgcca tgcgaaggac
      841 gtgatcggcg cccggtcggt ggccttgggc gagggtgaag tggatgtcac cgggtgcctg
      901 cgggtgctcg agaaccacgg ctacgagggc gcgatctcgc tggagaccga gggtgaggag
      961 gcgcccgacc agatgcagca gctcatcgaa cgcagtcgtc gcttcctcga gcacgcactg
     1021 gggccccgct agatctggga ggcgatcggg cccgaacaga actccagccc ctcgcccgtg
     1081 tgcccgcgcg ccctactgga cgatcagcag cgagtcatcc agaccctccg ccgtggtgaa
     1141 ctcccgcacg cccgaacccg ataccgtgcc gatgcggcgg ctctgcttag cgatccctcc
     1201 actgaagccc gcccctggac gtaccggcaa ccggacggga tgccgcgcgc gctcgccctc
     1261 ggctcccctg ggggggtgtt cgcagaccac atcgacggca gtcttctctc gcacggcgcg
     1321 ggcccggccg ctcctccctc gagcatgccg ggaggaccgt ctggcaggcc gacggaactc
     1381 tgagcccgcc ggaccgacta actccgtgcc cgtgtctgga gagcggagtg ggccgagagg
     1441 acgttgcgca tggacggcca ggggatctcg cgcaggacct tcatacgcac cgccgccgtg
     1501 gcggcaggct ttgcggccgt ggccggaccc acgccggccc ggtgccagga gcgtactctg
     1561 cccaagcgcc ggatggcggg cacggaactc gacgtgagtg tgctcggcta cggcaccgag
     1621 ttcatgcagg accgggccgt ggccgaccat ctcgtctcgt tcggcgtcaa ccacatcgac
     1681 accgcacccg tgtaccagga gggcgaggcc gaacggcggc tcgtaccggt gatccgagac
     1741 cacggggatt cgctggtgct aaccacgaag tgggggcacc actcggacct ggccgcgccc
     1801 aaggacgagt tcctggccag cttcgccgcg agttgcgagc gcatggagac ggaccatgtg
     1861 gacatcatgt atgtccatga cgcccgcacg ccggagaagg cggcgttcgc cagcccctat
     1921 gaggcgttca gagagctacg cgacgccggc aagataggat attacggact gagcacccat
     1981 cttgggcagg aagcgaccgt gctcaagtgc atcgagcttg gctggtacag cgtgctcatg
     2041 gtcgcctgga acttcttctc tcctccgtcg ctcaccgacg ccctcaaaag ggccgccgac
     2101 aacggcatgg gcatcgtggt catcaaggcg ctgaagggcc tcagtgaagg tcaggactgg
     2161 ttccagcgcg cgacgccaga gcagcgcgcc gtgctggaca ccttcgaggg gtccatgtac
     2221 cagcgcgcca tccggtggac gctctcgcac gacttcgtgt ccagcatcat catgtcggta
     2281 tcgaacctgg accaggtgga ggagaacgtg gtcgggggat tcgaggcgtc ccgggtgggt
     2341 gacgcccggc agctgcgacg atacgcccgt gccgtctcga accaggtgtg tcggggctgc
     2401 gccacgtgtc ttggtgcctg ccctgtaggg gtggccgtgc ccgacatcct gaggtaccgc
     2461 gcgtaccatc gggcatatgg tgaagcacac acggcacggc gactatacgc ggacctgccc
     2521 gagagcatgc gagccaacgc ctgcaccgga tgcggcatct gtgccgccca ctgcccctac
     2581 ggcctcgccg tgcctgagct gatcgccgag gcacatcggc tgctggccta gtgaggctcc
     2641 ggggccggaa ccgcccgttc cccgaaggtc gcgctagtcc atctgctggc cgccagtgac
     2701 gttgatggcc tgaccggtca tatagctcga gtcatccgag gccaggaact ccaccacgtt
     2761 ggccacgtcc gcgtactcgc aaccccggcc catcggaacc tgctgctcgt acttggcgcg
     2821 gacctcctcc acggtgatac cccaccgctg ggcatactgg ccatacaggc tatcgaccca
     2881 taggggcgag tcgagaaggt tcccggggca gaccgagttg acgcggatcc catcgggcgc
     2941 cagctccagg gccaggctct gcgtcaggcc gatcccgccg aacttgctgg cggcataggc
     3001 tgagttgcgg aagctgccct tcttgcccga cttcgagttg atctggatga tgacaccgct
     3061 ctgctgcggt accatgaccc gagccgcctc gcgggcacag acgaagtaac ccacaaggtt
     3121 cacgtcgatg accttgcgcc atcggtcgat ctcaatatct gtcaggccgc cggcgaaaag
     3181 aatgcccgcg ttgcacacca ggatgtccag gcggccccac tcgtcaacgg cccgcc
//
LOCUS       DAOSPL010000391         3232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29846_length_3232_cov_3.039031, whole genome shotgun sequence.
ACCESSION   DAOSPL010000391 DAOSPL010000000
VERSION     DAOSPL010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29846_length_3232_cov_3.039031"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PLQ54_08295"
     CDS             complement(<1..417)
                     /locus_tag="PLQ54_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOS93297.1"
                     /translation="MGAVRVIPCLDIKDGRVVKGVHFVELRDAADPVEAARAYSEGGA
                     DEIAFLDITATVEGRRTVFDLVTRVAEAISVPLTLGGGIKSLADVGTALAAGAAHVSI
                     SSAAYRDPGMVREAVARYGSDTIIVAIDADANPSCPS"
     gene            626..1381
                     /locus_tag="PLQ54_08300"
     CDS             626..1381
                     /locus_tag="PLQ54_08300"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOS93298.1"
                     /translation="MRVLVLCGVLGLAAVTGPSPAADEATVVERIRAVLWVGGFAHDF
                     DAIAEITREFLPRQAPIDISVVHDGGFLDEAELPDLIIMNHCIESTEGILTEARQKRL
                     LDAIRGGVGVVAIHASYYSFTKWPEFRELFGATFMQHAESDIQIEVRVVDAKHPVAAG
                     IPTTFEVHSELYESTPLADDCRLIAVAKQVGGDKEWPSVWAKPYGKGRVVTLLPAHWP
                     DAYRVDAFQRLIVNACLYAAGRGAAAEAAEEAK"
     gene            1381..2433
                     /locus_tag="PLQ54_08305"
     CDS             1381..2433
                     /locus_tag="PLQ54_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS93299.1"
                     /translation="MDPTRFSRRDFLRATSAAALAASLAPAGGADAQTTSTPLNDNGR
                     MKYRRLGKTEFMISEVSLGGHGGSSVDDRVAVLDKAVELGINYVDNNIDTECDLYGQA
                     MAASAHAQREKWFIGFASWPEKITTEYEDQLTPDGMMANIEARLQSYRTDMLDLWRPV
                     GATWGEGQTDMATMLMVSDKTLDMVVQVFEKAREQGKVRFLGISAHNPKVFRRVLEAY
                     PQFSVVIFPYLFLTEEFGGGSLLELAKAKDVGVIGLKPFGAGTIFAQRPSEIQGRVDA
                     RARVLIKEMLREPRISAVIPGVTAPEQLEENVLGSYEREAMATPEDRVILAQCAEAFE
                     RNLTPEYHWLREWRVV"
     gene            2430..2927
                     /locus_tag="PLQ54_08310"
     CDS             2430..2927
                     /locus_tag="PLQ54_08310"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOS93300.1"
                     /translation="MRAHWSWIAATAVALCLAALAQDQDAPPPAPEPTAPADTDVLDP
                     MGPNGACYVCHMTFLREDLAKTHLKAKVTCIRCHGLSAAHANDENIGATKPDRLYERT
                     QVSDSCRECHATHEAAPERVIQLWLDRELTEVPPVCTDCHGTHRIDAPAPQQPEVPPT
                     QGGAG"
BASE COUNT          546 a         1104 c         1078 g          504 t
ORIGIN      
        1 cgacgggcag ctcgggttgg catcggcatc gatggccacg atgatggtgt cgcttccgta
       61 ccgcgccacg gcctcgcgga ccatgcccgg gtctcggtag gccgcgctcg agatcgagac
      121 gtgcgcggcg ccggcggcca atgccgtgcc gacatccgcg agcgacttga tgccgccccc
      181 cagcgtcaga ggcacgctga tggcctcagc cacacgcgtc acgaggtcga acacggtcct
      241 tcgtccctcc acggtggccg tgatgtccag gaacgcgatc tcatcggcgc cgccctctga
      301 gtacgctcga gccgcctcga ccgggtcagc cgcatcacgg agctcgacga agtgaacacc
      361 tttcaccacc cggccatcct tgatgtccag gcacgggatc actctcacag cacccatggt
      421 ggaagctccc tccggcccgc ccgacgccat gccggccgaa gcggcacggg cacgtgcggg
      481 ccaaggcttg ggttcggcgg ggcgcgggcg ccgtcctccg cctggtctcg ccatgcggat
      541 gtgtgcggga ggcctcgccg cgcgagccgc gaatgcgttg ggtggccgcc gaccggcggc
      601 gcccgatgta ggacgggagg ccaccatgcg tgtactggtg ctgtgcggag tgctggggct
      661 cgcggccgtc accgggccga gtccggccgc tgacgaggcg accgtcgtcg aacgcattcg
      721 cgctgtgctg tgggtcggcg gcttcgccca tgacttcgat gccatcgccg agatcacacg
      781 ggagttcctg ccgcggcagg cgcccatcga catcagcgtc gtgcacgacg gcggcttcct
      841 cgacgaggca gagcttccag atctgatcat catgaaccac tgcatcgagt cgaccgaggg
      901 catcctcacc gaggcgcggc agaagaggct actcgacgcg attcggggcg gtgtcggtgt
      961 ggtggccatt cacgcctcgt actactcatt cacgaagtgg cccgagttcc gcgagctctt
     1021 cggggccacg ttcatgcagc acgccgaatc cgacatccag atcgaggtgc gtgtcgttga
     1081 cgcgaagcac ccggtggccg cggggatccc aacgaccttc gaggtgcaca gtgagctgta
     1141 cgagtcgacg cccctcgctg acgattgccg cctcatcgcg gtggcgaagc aggtcggcgg
     1201 cgacaaggaa tggccctcgg tctgggccaa gccctatggc aaaggtcgcg tggtgaccct
     1261 gctgccggcc cactggccgg acgcctatcg cgtggacgcg ttccagcgcc tgatcgtgaa
     1321 cgcttgcctc tacgcagcgg gacgaggtgc ggccgccgag gccgcggagg aggccaagta
     1381 gtggacccga cacgcttcag cagacgcgac ttcctgaggg ccacgtcggc ggccgcattg
     1441 gcggcctcac tcgcgccggc gggtggggct gacgcccaaa cgacgtccac accgctgaac
     1501 gacaacggtc ggatgaagta ccggcgactg ggtaagactg agttcatgat ctccgaggtc
     1561 agcctcggtg gccatggcgg gtcgtctgtt gacgatcgcg tggcggtgct cgacaaagcc
     1621 gtcgagctgg gcatcaacta cgtcgacaac aacatcgaca ccgagtgcga cctatacggc
     1681 caggccatgg ccgcatcggc tcacgcccag cgcgagaagt ggttcatcgg cttcgcctca
     1741 tggcccgaga agatcaccac cgagtacgag gaccagctca cgcccgacgg catgatggcc
     1801 aacatcgagg cccggctcca gtcgtatcgc acggacatgc tcgacctgtg gcgtcccgtt
     1861 ggcgccacat ggggcgaggg ccagaccgac atggccacca tgctgatggt cagcgacaag
     1921 acgctcgata tggtcgtgca ggtgttcgag aaggccagag agcagggcaa ggtgcgcttc
     1981 ctcgggatct ccgcgcacaa ccccaaggtg ttccggcgcg tgctcgaagc ctatccgcag
     2041 ttctccgtcg tcattttccc atacctgttc ctgaccgagg agttcggcgg cgggagcttg
     2101 ctggagctgg cgaaggcgaa ggatgtcgga gtcatcggcc tgaagccctt cggcgccggc
     2161 accatcttcg cgcagcgccc cagcgagatc cagggccggg tcgatgcgcg ggcccgggtg
     2221 ctgatcaagg agatgctccg cgagccgcgt atctcagccg tcatacccgg cgtcacggca
     2281 cccgagcagc tcgaggagaa cgtgctcggc tcctatgagc gcgaggccat ggccacaccc
     2341 gaagatcgtg tcatcctcgc ccagtgcgcc gaggctttcg agcgcaatct gacgccagag
     2401 taccactggc tacgcgagtg gagagtcgta tgagggcgca ctggagttgg atcgccgcca
     2461 ctgccgttgc gctgtgcctg gcggccctgg ctcaggacca ggacgctccg ccgcccgcgc
     2521 cggagcctac ggcgcctgca gatacggacg tcctcgaccc catggggccg aacggggcct
     2581 gctatgtctg ccacatgacg ttcctgcgcg aggatctggc gaagacgcat ctcaaggcga
     2641 aggtaacctg tatccggtgc cacgggctga gcgcggcgca cgcgaacgat gagaatattg
     2701 gagccacgaa gcccgaccgc ctctacgaac gcacccaggt gagcgactcg tgccgcgagt
     2761 gccacgcgac tcacgaggcc gcgccggaga gggtcatcca gctctggttg gaccgcgaac
     2821 tgaccgaggt gccgcccgtc tgcacggatt gccacggcac gcaccggatc gatgccccgg
     2881 caccacagca gcctgaggtg ccgccgaccc aaggcggggc gggataggcg gccgcacgcg
     2941 caggatccgg agccgcctga ccggcgacca tcccacgcga ccacagtccc cggccgggct
     3001 tggcctcgcg tccttcgtgt gcttcgcagc tgacagtgat cctcgctctg cccgcgctac
     3061 gcgccgactc tgcccatcac ctcggccacg atggcctccg ccgtgattcc gtgctcgcgc
     3121 ttcacatcgg cgtggctgcc cgacatgatg gcgaacacgt cctgcaggcc caggcgacgc
     3181 aggggcgtcg cggagcccgc ctcggccagg atctcggcca cggcgctccc aa
//
LOCUS       DAOSPL010000392         3229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29868_length_3229_cov_2.846251, whole genome shotgun sequence.
ACCESSION   DAOSPL010000392 DAOSPL010000000
VERSION     DAOSPL010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29868_length_3229_cov_2.846251"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1251
                     /locus_tag="PLQ54_08315"
     CDS             172..1251
                     /locus_tag="PLQ54_08315"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOS93301.1"
                     /translation="MLRLVYRYLASELIVPFFSWIIGLVLLLVGNFLFLLLKSAEGRP
                     LPWDLTLRYLVFRVPFALVLCAPMAFLFAVALTINRFARDGELTGLRMGGMGPRRILL
                     PFFAAGLLLSVIDFTIYDAVVPWANHVSDNAVRGILLQQAELKPSEDLVIKPPDGDVI
                     FFAKDMDLAHDLLKGVVLCRLLPQSFPEFVVASQAELKDNVWHLSDGCMFQFDDEGYL
                     RAGMVADRVTIDLKEVFAEYLEDRWNPEQMSVGQLSQRVHELKRGGRKASMEISEMHS
                     KFAIPFACLAFTLLAGPLALRFGTGASAFTGLLMAAVVLFIYYLLLAWSKVLGPTGRM
                     DPVLAAWMPNAVFAVLGAVALVRSR"
     gene            1272..2033
                     /locus_tag="PLQ54_08320"
     CDS             1272..2033
                     /locus_tag="PLQ54_08320"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOS93302.1"
                     /translation="MTRCPKCLRQFGDQCTECPECGEPTVRGLAPLGPVEKREERLVC
                     VHRLEDRVAAGAIMGLLEQAGIRAIVREDSAEHVFGVSSLGVGRYGGICVLEPDAVRA
                     TELIEEYLRAVGSPESTGEEGDGETAMTHCPRCLQCFADHCTECPDCGQPTVRGPVPI
                     GRDRKREQRLVSVYQPTDALEAGNIVAILEQGGLRATAIEDRTVNLRVAPPPAITPWG
                     VVCVLEHDASRAAQLIEEYLRAVSAPGASSDPEAG"
     gene            2033..3010
                     /locus_tag="PLQ54_08325"
     CDS             2033..3010
                     /locus_tag="PLQ54_08325"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HOS93303.1"
                     /translation="MAASRSVAFALLLSVALPAGAGSATEPFEMSRDGFTIQYHARRR
                     SATGEIFRVLSEARAAVSARLGTEAPPPVTVRFCESAQEFHESIGLRVPWYVAAVALP
                     EERRIAVEWDVAFPSLRQVLAHELCHIYLHESLGPHARAVPLWMEEAIAKHVSGEWTE
                     SDEVVLQEAVLGGKLLGFGSIERDFPSQHRAATVAYAQSATFLAFAEQRHGPLVLRAY
                     LDGVRGGLGHREALEAATGRDLDSLEAEWKEYLRLRFRRRMLADRVDELIFSFMAVLL
                     LVCAGVVWVRRRRWLAEQDAEEFPEELGNGPSMTGYPPRRRGRYFGPRF"
     gene            3031..>3229
                     /locus_tag="PLQ54_08330"
     CDS             3031..>3229
                     /locus_tag="PLQ54_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93304.1"
                     /translation="MPVFLIFLLSQVSAPSMLADAQRLPVIQGARFPALSPDAKEIAF
                     SYRGDLWVADIASRVARRLTVS"
BASE COUNT          474 a          978 c         1155 g          622 t
ORIGIN      
        1 aaccctgact tcatcgtgat gggggacccg gatctcgcct ggggcaagat cctggggcac
       61 gccggccgta tggacccggc gcttggcgcg tggatccaga acatcgtgta cctcgtcgcc
      121 ggttccttcc tgatctggcg cagcgagtga ggaggccgga ggggggcgga catgctcaga
      181 ctcgtgtacc ggtacctggc ctcggagctg attgttccct tcttctcgtg gatcatcggc
      241 ctggtactcc tgctcgttgg caacttcctg ttcctgctgc tcaagtcggc cgaggggcgc
      301 cccttgccct gggatctgac actgcggtat ctggtcttcc gcgtcccctt cgcgctggtg
      361 ctgtgcgcgc ccatggcgtt cctgttcgcg gtggccctga cgatcaaccg gttcgcccgc
      421 gatggagagc tcactggcct gcgcatgggt gggatgggac cgcggcgcat cctgctgccc
      481 ttcttcgccg ctggcctctt gctctcggtg atcgacttca ccatctatga tgccgtggtg
      541 ccgtgggcca atcacgtgtc tgacaacgcc gtccgaggca tactcctcca gcaggcagaa
      601 ctcaaaccca gtgaggacct ggtgatcaag ccgccggatg gcgatgtgat cttcttcgcc
      661 aaagacatgg acctcgcgca tgatctgctc aagggggtcg tcctctgccg gctcctgccc
      721 cagtcgttcc ctgagtttgt ggtggcgagc caggctgagc tcaaggacaa cgtctggcac
      781 ctctcggatg ggtgcatgtt ccagtttgat gatgagggct atctgagggc cgggatggtg
      841 gccgataggg tcaccatcga cctgaaggag gtgtttgccg agtacctgga ggaccgatgg
      901 aacccggagc agatgagtgt cggtcagctc agccagaggg tgcatgagct gaagcgcgga
      961 ggtcgaaagg cctcgatgga gatcagcgag atgcactcga agttcgcgat tccgttcgcg
     1021 tgtctggcgt tcacactgct ggccggtccg ctggcattgc ggttcgggac gggcgccagc
     1081 gccttcacgg gcctcctgat ggcggcagtt gtgctcttta tctactacct tcttctcgct
     1141 tggagcaagg tgctcggccc cacgggaagg atggacccgg tactggcggc ttggatgccc
     1201 aacgcagtct tcgccgtgct gggcgcggtc gcgttggtgc ggagccgcta acggccgaat
     1261 gggaggccgc catgacgcgt tgccccaagt gcctgcggca gttcggcgat cagtgcacgg
     1321 agtgccccga gtgcggagag cccacggtca gggggctcgc ccctcttgga ccggtcgaga
     1381 agcgtgagga gcgattggtg tgcgttcacc gtctcgagga ccgcgtggcg gcgggggcca
     1441 tcatgggtct gctcgagcag gcgggcatcc gggccatcgt gcgcgaggac tcggcggagc
     1501 acgtcttcgg ggtctcctcg ctcggggtgg gccgctacgg cgggatatgc gtcctcgaac
     1561 cggatgccgt tcgagcgacc gagctcatcg aggagtacct tcgggcagta gggagccccg
     1621 aatcgacggg ggaggagggg gacggggaga cagccatgac gcattgcccg cggtgtctac
     1681 agtgtttcgc cgaccactgc acggagtgtc ccgattgcgg acaacccacg gtgagggggc
     1741 ctgttccgat cgggagggac cgtaagcgag agcagcgcct ggtgagtgtc tatcagccca
     1801 cggacgcgct cgaggcgggc aacatcgtgg cgattctgga gcaaggaggc cttcgggcga
     1861 ccgcgatcga ggaccgcacg gtgaacctgc gggtggcgcc ccctccggcg atcaccccgt
     1921 ggggggttgt ctgcgttctg gagcacgatg catcgagggc cgcccagctc atcgaggagt
     1981 acctgcgggc ggtgtcggcg ccgggggcga gcagcgaccc ggaggcaggc tagtggcggc
     2041 gtctcgatcc gtggcgttcg cgctgctgct gtcggtcgca ctgccggccg gggcaggatc
     2101 cgccacagag ccctttgaga tgtcacgcga tggtttcacc atccagtacc atgcccgccg
     2161 gcgttcggcg accggcgaga tcttccgggt actgagcgaa gcccgggcgg cggtgagcgc
     2221 ccggctgggt accgaggccc cgcccccggt gacggttcgc ttctgcgagt ccgcgcagga
     2281 gttccacgag tccattgggc tgcgcgtgcc ctggtacgtg gccgcagtgg ccctcccgga
     2341 ggaacggcgg atcgccgtgg agtgggacgt ggcattcccg agtttgcgcc aggttcttgc
     2401 ccacgagctc tgccacatct acctccacga gtcccttgga ccgcacgcgc gagctgtgcc
     2461 gctgtggatg gaggaggcga tcgccaagca cgtgtccggc gaatggaccg agagcgacga
     2521 ggtagtcctc caggaggccg tgctcggtgg taagcttctc gggtttggat cgatcgagcg
     2581 cgacttcccg tcgcagcacc gggccgcgac ggtggcgtat gctcagagcg ccacgttcct
     2641 cgccttcgcg gagcagcggc acgggcctct ggtgctcaga gcctatctgg atggcgtgcg
     2701 cggaggcctg ggccatcgcg aagcgctgga ggcggcgacg ggtcgggatc tggactcact
     2761 ggaagctgag tggaaggagt acctgcggct ccgattccgc cggcggatgc tggcggaccg
     2821 cgtggacgaa ctgatcttca gcttcatggc ggtgctcctt ctcgtgtgtg ccggcgtggt
     2881 gtgggtgcgg cggcggcgct ggctggccga gcaggacgcc gaggagttcc cggaggaact
     2941 ggggaacggc ccaagcatga ccggctatcc acccaggcgt cgtgggcgct acttcggccc
     3001 gcgtttctga tgatacgagg agcggtatcc atgcccgtgt tcctgatctt cctgttgtcg
     3061 caggtctcgg cgccatcgat gttggccgat gcgcagcgcc tccctgtcat ccaaggcgct
     3121 cgttttcccg cgctgtcgcc ggacgccaag gagatcgctt tcagctaccg tggtgacttg
     3181 tgggttgcgg acatcgcgtc gcgtgtggcc cggcgcttga cggtgagtg
//
LOCUS       DAOSPL010000393         3228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29876_length_3228_cov_3.466751, whole genome shotgun sequence.
ACCESSION   DAOSPL010000393 DAOSPL010000000
VERSION     DAOSPL010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29876_length_3228_cov_3.466751"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PLQ54_08335"
     CDS             complement(<1..634)
                     /locus_tag="PLQ54_08335"
                     /inference="COORDINATES: protein motif:HMM:NF022727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3084 domain-containing protein"
                     /protein_id="HOS93305.1"
                     /translation="MTPTVIIVAIALVAAGGLIAYIGDTIGKRLGRRRATLFGLRPRW
                     TAILVSTTTGVLIALMSLGILLTFSGRVREALTRYDALKSDIDQAQKDLSAAKQAVAA
                     ARQSAVRAREQRDDALSARREAEGALAKTENELGAATENLSEREGQLRSAEGELKRKR
                     IELSASQAAASSLAYGVKYELFDSRPVTFPRGDELARTTLTAGLSKAEIRS"
     gene            complement(665..1789)
                     /locus_tag="PLQ54_08340"
     CDS             complement(665..1789)
                     /locus_tag="PLQ54_08340"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOS93306.1"
                     /translation="MIRILDRYVVRELLPLVVVGTALFGGIIILFSPLKEALELAQVT
                     GLSQGTMWRVLGLRSLGVVSFCLPMGVLFGAALLSGRWASTGELLAAHSAGVAVGSLM
                     RWVFVTGALVGVVGVAFHEYVAVPGLASARRVEDGAQFGKRARGRHNVHFLEADENTG
                     APRYLIFAREMDLDSQTLTGVTALVFPPAPAAAEPSRPTILQAAQTVYLGDGKWEARR
                     IYAWGPTDTRPVPLATQVLEVGRAPRDLVHDKREIQATAARQLQQRIARLAPQARVEP
                     DVARLVAQLRMELYGRWSVPLACLPFALLGAPLGARRQGGGTSLALGLSVLVCFLYYT
                     VLRYAEMAGREGYLPPAFAAALTPALALVLGAALLYRAQR"
     gene            complement(1786..2616)
                     /gene="lptB"
                     /locus_tag="PLQ54_08345"
     CDS             complement(1786..2616)
                     /gene="lptB"
                     /locus_tag="PLQ54_08345"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018860724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOS93307.1"
                     /translation="MADVNRDHAVLVGTEESAPPSLGGGGLRGEQLVKSYGRRTVVDG
                     VSLDVKPGEVVGLLGPNGAGKTTTFYMLVGVVRANAGRVFIGDQDVTRLPMHRRARLG
                     IGYLPQHASIFQKLTVWENVMAIAELMPISREERVERVQRVLARFGVDGLSSSRGYQL
                     SGGERRRVEIARCLASEPKFMLLDEPFTGVDPKAVRDIQSIVQGLRDDGIGVLITDHS
                     VRETLDIADRAYIIEGGSILTEGKPEELKRDELAARAYFGDVFAHLLQAKHEEPPEAP
                     "
     gene            complement(2674..>3228)
                     /locus_tag="PLQ54_08350"
     CDS             complement(2674..>3228)
                     /locus_tag="PLQ54_08350"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOS93308.1"
                     /translation="RVTGFHHSEPVAWLSGRLFSELAALHELGTTSELTLHAAAVLHD
                     IGWVQGKRKHHKVSMQMILETPIACLDAHEQRRVACLARYHRRAFPDAGHAVFGNLSR
                     DEQDEITRLAALLRVADGLDRGQVGRVRGVRTLRIDPASVNLELRAASHCPEELLSAT
                     RKSDLFRHVFGRALFVRWRPDGPR"
BASE COUNT          555 a         1135 c         1059 g          479 t
ORIGIN      
        1 ccgaccggat ctccgccttg gacagtccgg ccgtgagtgt agtgcgagcc agctcatcgc
       61 cacgcgggaa ggtgacgggg cggctgtcga agagctcata cttcacgccg taggccaggc
      121 tcgaggccgc cgcctggctg gccgagagct cgatccgctt gcgcttcagc tcgccctccg
      181 cactgcggag ctgcccctcg cgctcgctca ggttctcggt cgccgcaccc agctcgttct
      241 cggtcttggc gagggccccc tcggcctccc ggcgggccga caacgcgtca tcccgctgtt
      301 cgcgggcccg cacggccgac tggcgggcgg ccgccactgc ctgcttcgcc gcgctgaggt
      361 ccttctgggc ttgatcaatg tcgctcttga gggcatcgta ccgggtcagt gcctcccgga
      421 cgcggccgga gaaggtgagc agaatgccca gagacatgag ggcgatgagt accccagtgg
      481 tggtgctgac gagaatggcg gtccagcgcg gccggagccc gaacagcgtg gcgcgacggc
      541 ggcccagccg cttcccgatg gtgtcgccga tgtacgcgat gaggccgccg gctgctacca
      601 gcgcgatcgc gacgatgatc accgtcggtg tcatgggccc tctcctgcct ggatggttgc
      661 gcggttaccg ctgcgcgcgg tacagcagtg cggccccgag gacgagtgcc agcgccggcg
      721 tcagcgccgc cgcgaatgcc ggggggagat acccctctct gcccgccatc tcggcatacc
      781 ggagcacagt gtaatacagg aagcacacca acacactgag tcccagcgcc agagacgtcc
      841 cgccgccctg gcggcgggcc ccaaggggcg cacccagaag cgcgaagggg agacaggcca
      901 ggggcacgga ccagcggcca tacagctcca ttcgcagctg cgccaccagt cgcgcgacgt
      961 cgggctcgac ccgtgcctgg ggagcgagcc gggcgatccg ctgctggagt tggcgggcgg
     1021 ccgtggcctg gatctcccgc ttgtcatgca ccaggtccct cggggcgcga ccaacctcga
     1081 ggacttgcgt ggccagcggc acggggcgcg tgtcggtcgg cccccaagcg tagatccgcc
     1141 gggcctccca cttgccgtcg cccaggtaca ccgtctgggc cgcctgcagg atggtggggc
     1201 gggagggctc ggccgctgcc ggggctggcg ggaagaccag ggccgtcact ccagtcagtg
     1261 tctggctgtc gaggtccatc tcacgggcga agatcagata cctcggggca ccggtgttct
     1321 cgtcggcctc caggaagtga acgttgtgcc gaccgcgcgc tctcttgccg aactgggccc
     1381 cgtcctcgac acgtcgggcg ctcgccaacc cgggcaccgc cacatactcg tgaaacgcaa
     1441 cacccaccac gcccaccaga gcgcccgtca cgaacaccca acgcatgagc gagcccacgg
     1501 ccactcctgc actgtgcgcg gccagcaact cgcccgtgct cgcccagcgt cctgacagca
     1561 gtgccgcgcc gaagagcacg cccatgggca agcagaagga cacgaccccc aggctcctca
     1621 gccccagcac tcgccacatg gtgccctgag acagcccggt gacctgagcg agttcgagtg
     1681 cctccttcag cggagagaac aggatgatga tgccgccaaa caaggccgtg cccaccacga
     1741 caagcggcag cagctcgcga accacatagc ggtcgaggat cctgatcacg gcgcctccgg
     1801 cggctcctcg tgcttcgcct gaagcaggtg ggcgaacacg tcgccgaaat aggcgcgggc
     1861 ggcgagctcg tcgcgcttca gctcctcagg cttaccctcg gtcaggatac tgccgccctc
     1921 gatgatgtag gcgcggtcgg cgatgtcgag ggtctcccgg acggaatggt ccgtgatcaa
     1981 cacaccgatc ccgtcatcgc gcaacccctg gacgatgctc tgaatgtcgc gcacggcctt
     2041 cggatcgaca cctgtgaacg gctcgtcgag cagcatgaac ttgggttcgg acgccaagca
     2101 acgcgcgatc tcgacccgac ggcgttcgcc tcccgagagc tgatagcctc gggaactgct
     2161 caatccgtcc acgccaaagc gcgccagtac ccgctggacg cgctcgacgc gctcctctcg
     2221 agagatgggc atcagctcgg cgatggccat gacgttctcc cagaccgtga gcttctggaa
     2281 gatcgatgca tgctggggca ggtagccgat gccgaggcgg gcacggcgat gcatgggcaa
     2341 ccgagtcaca tcctgatcac cgatgaagac tcggccagcg ttcgcgcgga cgacgcccac
     2401 gagcatgtag aacgtggtcg ttttccccgc cccgttgggc ccgagcagac ccacgacctc
     2461 gccgggcttc acgtcgaggg agacgccgtc gacgaccgtc cggcgtccat aggacttgac
     2521 gagctgctcg ccgcgaagac ccccgccgcc aagtgaagga ggcgcgctct cctcagttcc
     2581 cacaagaact gcatgatcac ggttcacatc ggccatgtcc ggatgttaga agccaccctc
     2641 ctgacaacca gacgaaccgg agcgagcgcg ccttcagcgc ggcccgtccg gccgccatcg
     2701 aacgaagagc gcacgcccga agacgtgccg gaacaggtct gacttcctgg tggcgctcag
     2761 cagttcctcg gggcagtggg acgccgcgcg gagctccagg ttgacagatg ccgggtcgat
     2821 gcgcaaggtc cgcacccctc gcacgcgccc cacctgcccg cggtccagcc catcggccac
     2881 gcgcagaagc gccgcgaggc gagtgatctc gtcctgctcg tcccgcgata ggttgccaaa
     2941 cacggcgtgg ccggcgtcgg gaaaagcccg gcggtggtac cgggcgaggc acgcgacacg
     3001 acgctgctca tgggcgtcca ggcacgcgat aggtgtctcg agtatcatct gcatcgatac
     3061 cttgtgatgc ttcctcttcc cctggaccca gccgatgtca tgcagcaccg cggccgcgtg
     3121 cagggtgagc tccgacgtcg tgcccagctc gtggagcgcc gcaagctcgg agaacaggcg
     3181 gcctgagagc caagccacgg gttcggaatg gtggaagccg gtcacccg
//
LOCUS       DAOSPL010000394         3226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29897_length_3226_cov_2.464207, whole genome shotgun sequence.
ACCESSION   DAOSPL010000394 DAOSPL010000000
VERSION     DAOSPL010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29897_length_3226_cov_2.464207"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="PLQ54_08355"
     CDS             <1..1234
                     /locus_tag="PLQ54_08355"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOS93309.1"
                     /translation="GFIMRHAATPWAPPTAQQIGLIGTHWSKGWIIEDNVVSHSVCVG
                     IALGKHGDPYDNTSANSAEGYVKTIERAHAFAIPWTKARIGHHVVRRNTISHCEQAGV
                     VGSLGAAFSTIEGNVIHDIHLRHLFGGAEMAGIKIHGAIDTVIRRNHVYRTCLGLWLD
                     WMAQGTRVSANLFHDNTGQDLFVEVNHGPFIVDNNLFLSSTSLLDMSEGGAYAHNLFA
                     GRITNCPEPNRETPYHRAHSTRLAGLARITGGDDRFYNNMFIGDGKALTGAEKGDVEE
                     VRWISGHGLWGYDGRPFPLEAAGNVYYHGAEPYAGEADPVVLLQHDPQLDLEVDEDGG
                     AHLSLRLTGLPGGTGPAIVTTALLGKTRVAGLPYENRDRSPLEIDTDYLGHRRSRSRS
                     VAGPFASLRGGSVRLRVW"
     gene            complement(1273..2340)
                     /locus_tag="PLQ54_08360"
     CDS             complement(1273..2340)
                     /locus_tag="PLQ54_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS93310.1"
                     /translation="MTPRERVLQAIDHRETDRVPIQIYLTPEMHGSLVERFAGRSPIE
                     VFEVDIRGIAAPYVGPARKPPPIATGKAELYDEWGIGYSAVANATGGVYHEASDLTLA
                     RLQTMDEVERYPWPNPDDYDYSAIPDAIARERPFCVHAGHAGAPDIINGVSRGRGMEQ
                     VLCDIALQDEVGCAIIDHRVDFYYEVLKRTLEAGRGEIDILWLGEDLGTQNGPVISPA
                     TFDAFFRPRLQRFIELAHHYGARAAMHSCGSTRALQPRLIEMGLDLLDAVQPEPAGMD
                     PEELKREFGDRLAYCGMISTQETLPHGTVEQCREEARHRLRVIAKGGGYIFSPAHCIQ
                     PDTPVENVVAIYEEALGRPLE"
     gene            complement(2410..>3226)
                     /locus_tag="PLQ54_08365"
     CDS             complement(2410..>3226)
                     /locus_tag="PLQ54_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93311.1"
                     /translation="SALDDARFYHLNTDREPVSYFYNLLLWDRFSESNFGSALRRLRA
                     AGVGTWLLGVAILVALRLAWGRARRWSRSTADRADSAVAIFACGLAAMGFEVVYLFSF
                     QNLFGSLYTRLALVVAAFMCGLALGGMWATKGLSLWARPPRTWLIGVCMASAAFAVGI
                     TPEVLSVARVLAASWPPVAAGVLVFGVALAGLLTGAAFPLAAAVHLESGSGATARSAG
                     AMQAADHLGGALGAAAIGSLLIPVLGVGSALWVIAAWSLAGAIVLVRRRHPQP"
BASE COUNT          541 a         1039 c         1164 g          482 t
ORIGIN      
        1 cgggttcatc atgcggcacg cggccacgcc ctgggctccg ccgacggccc agcagatcgg
       61 cctgatcggc acccactgga gcaaggggtg gatcatcgaa gacaacgtcg tcagccactc
      121 ggtctgtgtc ggcatcgcgc tgggcaagca cggcgacccg tacgacaaca catcggccaa
      181 cagcgccgag ggctatgtga aaaccatcga gcgagctcac gcatttgcca tcccgtggac
      241 gaaggcgcgc atcggccacc acgtggtgcg ccgcaacacg atctcgcact gcgagcaggc
      301 gggcgtcgtc ggcagcctcg gtgccgcgtt cagcaccatc gaggggaacg tcatccacga
      361 catccatctg cgccacctgt tcgggggcgc cgagatggcc ggcatcaaga tccacggcgc
      421 cattgacacg gtgatccggc gcaaccacgt ctaccgcacg tgcctggggc tctggctgga
      481 ctggatggcc caggggacac gggtgtcggc caacctgttc catgacaaca cggggcagga
      541 tctgttcgtc gaggtcaacc acgggccatt catcgtcgac aacaacctgt tcctgtcgtc
      601 caccagcctg ctcgacatgt cggagggtgg ggcctatgcc cacaacctgt tcgcgggacg
      661 gatcacgaac tgccctgaac cgaaccgtga gacgccttac caccgtgcgc acagcacgcg
      721 gctggccggg ctcgcccgga tcacgggcgg cgatgatcgc ttctacaaca acatgttcat
      781 cggcgacggg aaggcgctca ccggggcgga gaagggcgac gtggaggagg tgcgctggat
      841 cagcggccat gggctctggg gctacgatgg ccggccgttc ccgctcgagg cggccggcaa
      901 cgtgtactac cacggcgccg agccctacgc gggtgaggcg gatccggtcg tcctgctcca
      961 acacgacccg cagctggacc tggaggtaga cgaggatggt ggggcccatc tgagcctgag
     1021 gctgacgggt ctgccgggtg gcacgggtcc ggccatcgtg acgacggcgc tgctcgggaa
     1081 gacccgagtc gccggcctcc cgtatgagaa ccgcgaccgg tcgcccctgg agatcgacac
     1141 cgactacctc ggtcaccgtc gcagccgttc gcggtcggtc gccggtccgt tcgcgtctct
     1201 tcgcggcggc tcggtgcgac tacgggtgtg gtgagtagcg gggctccgtg ctcagccctg
     1261 ggccgccccc ggctactcca acggcctgcc cagggcttcc tcatagatgg cgacgacgtt
     1321 ctcgacgggg gtgtcgggct ggatgcagtg ggcgggtgag aagatgtagc caccgccctt
     1381 ggcgatgacg cggagccggt gccgagcctc ctcacggcac tgctcgaccg tgccgtgggg
     1441 aagtgtctct tgcgtggaga tcatcccgca gtaggccaag cggtcgccga actcgcgttt
     1501 gagctcctcg gggtccatgc cggccggctc gggctggacg gcatcgagca ggtcgaggcc
     1561 catttcgatg agtctcggct ggagcgcccg ggtggagccg caggagtgca tggccgccct
     1621 ggcgccgtag tggtgggcca gctcgatgaa ccgctggagg cgcgggcgga agaaggcatc
     1681 gaaggtggcc gggctgatga ccgggccgtt ctgggtgccg agatcctcgc cgagccagag
     1741 gatgtcgatc tcgccgcgtc cggcttcgag ggtccgcttg agtacctcat agtagaagtc
     1801 gacgcgatgg tcgatgatgg cgcagccgac ctcgtcctgc agtgcgatat cacacaggac
     1861 ctgttccatg cctctcccac gcgagacccc gttgatgatg tctggtgctc cggcatggcc
     1921 ggcgtggaca cagaagggcc gctcccgggc gatggcatcg gggatggcgg agtagtcgta
     1981 gtcgtctggg ttgggccacg ggtagcgctc gacctcgtcc atcgtctgga gccgcgccag
     2041 ggtgaggtcg gaggcctcgt ggtagacccc gccggtggcg ttggccacgg cggagtagcc
     2101 gatgccccac tcatcgtaga gctcggcctt gccggtggcg atgggcggag gcttccgtgc
     2161 gggaccgacg tagggcgcgg cgataccgcg gatgtcgact tcaaacacct cgatgggcga
     2221 gcggccggcg aaccgttcga cgagggaccc gtgcatctcg ggcgtcaggt agatctgaat
     2281 ggggacccgg tcggtctctc tgtggtcgat ggcctgtagt acgcgttcgc gcggggtcat
     2341 ggtcggcgac acccttccgc ggggtatctg gctcgcccgg cggctgagca ccggacgaag
     2401 ggcgattcgc tagggctgcg gatgacgcct gcggaccagc acgatcgcgc cagccagtga
     2461 ccaggcggcg atcacccaga gggcggaacc gacaccgagc acggggatca gtaggctgcc
     2521 gatggcggcg gcgccgagcg ccccgccgag gtggtcggcc gcctgcatcg cgccggcaga
     2581 gcgggcggtt gcgccggatc ccgattcgag atgcacggcg gcggccagcg ggaacgcagc
     2641 gcccgtcagg aggcctgcca gggcgacgcc gaacacgagt actcctgccg cgaccggggg
     2701 ccagctcgcc gcgagcactc gggccaccga cagcacctct ggggtgatgc cgacggcgaa
     2761 ggcggccgag gccatgcaca ccccgatgag ccacgtgcgg ggtggccgtg cccagagcga
     2821 gaggcccttc gttgcccaca tgccaccgag ggccaggccg cacatgaagg cggctacgac
     2881 gagggcaaga cgggtgtaga gcgatccgaa gaggttctgg aaactgaaga ggtacacaac
     2941 ctcgaagccc atggcggcga ggccgcaggc gaagatggcg acggcggagt cggcccgatc
     3001 tgccgtgctc cgcgaccacc gcctcgcccg gccccaggcc agccggagcg ccaccaggat
     3061 ggcgactccg agcagccacg tgcctacacc ggctgcgcgg agtctccgga gcgcggaccc
     3121 gaagttggac tcggagaacc ggtcccacag cagcaggttg tagaagtagg acaccggctc
     3181 acggtcggtg ttgaggtggt agaagcgggc gtcgtccagg gccgag
//
LOCUS       DAOSPL010000395         3218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_29999_length_3218_cov_3.024976, whole genome shotgun sequence.
ACCESSION   DAOSPL010000395 DAOSPL010000000
VERSION     DAOSPL010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29999_length_3218_cov_3.024976"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1087
                     /locus_tag="PLQ54_08370"
     CDS             170..1087
                     /locus_tag="PLQ54_08370"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOS93312.1"
                     /translation="MDQVTQLFVHNVLPVLCIMAAGYVAQKRLNLDIVTLNRLNLYVF
                     VPGLLLACLMNPGLDLGASGVIAVLCAAHQVALLGLGWFLCRVFRVDRDSAATASLAA
                     MSGNCGNFGIPLIELAFGPAGVALQAVVIAISNLGCYTLGVAAVSYGKHKTGGLVASL
                     LRLPSLYAVALALALRHCGRALPGPVAVAVDYVYRGLVPVALVTLGAQLAAARGRGQP
                     VPLTVAVVLRLVVAPLMMYGLVRLVGVNGLAARVLVVGAAVPAAVNTVVLAVEFDNQP
                     SLAAHIVLVTTLLAAVTVSVALYGVSIAL"
     gene            1162..>3218
                     /locus_tag="PLQ54_08375"
     CDS             1162..>3218
                     /locus_tag="PLQ54_08375"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS93313.1"
                     /translation="MVDLQTRFVGIDLSSPILVASAGITEKVDLLRRAQEHGAAGAVM
                     KSLFERETSRQSPSPRFRVIKHDLKRRPTFSLYSYEQASEWGPERYAEEVHRATEELD
                     MLVIPSINCLTPEGWARYARAMEEAGARALELNTSCPHGSITFSGTEVEKTIYESVRA
                     AREATTIPLIVKLSPMVTNPGNIAAELERIGVQAVTLFNRSTGLEVDVEAERPVMHGG
                     YAGHGGPWAIQYPLRWISQLAPQLGIDIAGTSGITSGEDVVKYLLCGAAVVQICTAVV
                     MNGYEAIGQIRRELTAWMERKGYRSPDEFRGRVCDRILGLEEVDRTTRWMARVARDVA
                     PPCQVDCPIGTSIQGYVQAIASGKPELAYEIASARNPFPSICGRVCHHPCEAACTRVD
                     IDDPLAIAALKRYAADRARELGIAPPTQPVRSTRPARVAVIGGGPAGLTAARELRRLG
                     YPVELFEAAPALGGMMMLGIPRFRLPKGIIEEEIQAVIDLGVAVHTGARLGRDFTVES
                     LRMQGFDAVLLAIGAHKPMPLGVPGEHYPHVLRGLDVLRGASLGQPIELAGHVVVFGG
                     GDVAFDAARTAVRLGAESVTVAYRRSSDEMPASRQEREDAQEEGVRILYLCAPLEVSP
                     GFVRCVRNRLGDVGTDGRRRPEPIEGSEFELAADWVIAAVGQEPDLAAQPELAALLER
                     LRAP"
BASE COUNT          464 a         1087 c         1108 g          559 t
ORIGIN      
        1 ccgagaaggc cccagagtag acgcgtgtcg acccctccgg gggtcaccag ttggcatcgg
       61 aagacgcagc tcgcccacgc ggcgggctgc gccttcttgt gcccgctctc gggccacagt
      121 cccgcggccc cggccgcccg tctgaagggg ctccgcccgc ttgcctgccg tggaccaggt
      181 tacgcagctg ttcgtgcaca acgtgctgcc cgtgctgtgc atcatggccg ccggctacgt
      241 tgcgcagaag cgcctcaatc tcgacatcgt caccctcaat cgcctcaacc tgtacgtctt
      301 cgttcccggg ctcctgctgg catgcctcat gaatccaggg ctcgaccttg gagccagcgg
      361 cgtgatcgcc gtgctctgcg ccgcccatca ggtcgcgctg ctggggctgg gttggtttct
      421 gtgccgcgtc ttccgcgtcg accgagacag tgcggctacg gccagcctcg cggccatgag
      481 cggcaactgc ggcaacttcg gtattcccct gatcgagctc gccttcgggc ctgcgggcgt
      541 ggccctgcag gcggtggtca tcgcgatcag caacctggga tgctacacgc tgggcgtggc
      601 agccgtctcg tacggcaagc acaagaccgg cggtctggtg gcctcgttgc tgcggctccc
      661 cagcctgtac gctgtggccc tggccctggc tctccgccac tgcgggcggg cgctccccgg
      721 ccccgtggcc gtggccgtcg actatgtcta tcgcggcctg gtgcccgtgg ctctggtgac
      781 attgggagcc caactcgctg cggcccgagg gcggggacag cccgtgccgc tcacagttgc
      841 cgtggtgctg cggctggtcg tggcacccct gatgatgtac gggctcgtgc gtctggtggg
      901 agtgaacggt ctcgcggccc gggtgctcgt ggtcggcgcc gccgtgcccg cggccgtcaa
      961 caccgtggtc ctcgccgtcg agttcgataa ccagccctcg ctggccgcgc acatcgtgct
     1021 cgtgaccacc ctgctcgccg ctgtcacggt gagcgtggct ctctacggcg tctccatcgc
     1081 cctgtagacg cccaccagtc ggctataatc ggccacgcgt tccaccagcc cgcgactccc
     1141 tccgcgagag gcgaatgcgc cgtggtcgat cttcagaccc gtttcgttgg catcgatctg
     1201 tcatcaccaa tcctcgtggc cagcgcaggc atcactgaga aggtggacct gctccgccgg
     1261 gcccaggaac acggcgcggc cggggcggtg atgaaatccc tctttgagcg tgagacatcg
     1321 cgccagtcgc cctcgccgcg cttccgggtc atcaagcatg acctgaagcg gcggcctacc
     1381 ttctctctct actcgtacga acaggccagc gagtggggcc ccgagcggta tgccgaggag
     1441 gtgcaccggg cgactgagga gctggacatg ctcgtgatcc cctcgatcaa ctgcctgacc
     1501 cccgagggct gggcgcgcta tgcccgcgcc atggaggagg cgggggcgcg agcactcgag
     1561 ctcaatactt cctgcccaca cggatcgatc acattcagtg gcacggaggt ggagaagacg
     1621 atctacgagt cggttcgcgc cgcgcgcgaa gccaccacca tcccgctcat cgtgaaactg
     1681 agccccatgg tgacgaaccc gggcaacata gcggccgagc tggagcgcat tggcgttcag
     1741 gccgtgacgc tgttcaaccg gtcgacaggc cttgaggttg acgtcgaggc cgaacgtccg
     1801 gtgatgcacg gtggctatgc cggccacgga gggccttggg cgatccagta cccactccgg
     1861 tggatcagtc agctcgcgcc ccaactgggt atcgacattg caggcaccag cggtatcact
     1921 tcgggcgagg acgtggtgaa gtacctcctg tgtggcgcgg ccgtggtgca gatctgcacc
     1981 gccgtggtga tgaacggtta cgaggccatc ggccagatcc ggcgagagct gaccgcgtgg
     2041 atggagcgca agggctaccg gagccctgac gagttcaggg gccgcgtgtg cgaccgcatt
     2101 ctggggctcg aggaggtcga ccggaccaca cgctggatgg ctcgggtcgc tcgcgatgtg
     2161 gccccaccgt gccaagtcga ctgccccatc ggcacgtcga ttcaggggta tgtccaggcc
     2221 atcgccagcg gcaagcccga acttgcctac gagatcgcgt cggcgcggaa ccccttccca
     2281 tccatctgcg ggcgcgtctg ccatcaccca tgcgaggcgg cgtgcactcg cgtggatatc
     2341 gacgacccgc tggcgatcgc ggcactcaag cgctacgccg cggaccgcgc cagggagctc
     2401 gggatcgcgc cgcctacgca gccggtgagg tccacgcgcc ctgcgcgggt cgccgtcatc
     2461 ggcggcggcc cggccgggct cacggcggct cgcgaactga ggcggctcgg gtacccggtc
     2521 gagcttttcg aggctgcccc cgcgctgggc ggcatgatga tgctgggcat cccccgattc
     2581 cggctgccca agggcatcat cgaggaggag atccaggcgg tcattgacct cggggtcgcg
     2641 gtgcacactg gcgcgaggct cgggcgggac ttcacggtcg agagcctgcg aatgcagggc
     2701 ttcgacgccg tgcttctcgc catcggagct cacaagccca tgccgttggg agtccccggc
     2761 gagcattacc cgcacgtgct gagggggctg gacgtgctcc gcggcgccag cctggggcaa
     2821 cccatcgaac tcgctggcca tgtggtcgtg tttggcggag gcgatgtggc tttcgatgca
     2881 gctcggacgg cggtacggct tggggcggag tcggtgaccg tggcctatcg gcggtcgtcc
     2941 gatgagatgc ccgccagccg tcaggagcgc gaggacgcgc aggaggaggg ggtgcgtatc
     3001 ctctacctct gtgcgccgct ggaggtgtct ccggggtttg tgcgctgcgt tcggaaccgg
     3061 ttgggcgacg ttggcaccga cggccggcgc cgtcccgagc ccatcgaagg ctcggagttt
     3121 gagctggccg ccgactgggt gatcgccgcg gtggggcagg aacccgactt agccgctcag
     3181 ccggagctgg cggcgctcct cgaacggctt cgtgcccc
//
LOCUS       DAOSPL010000396         3211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_30065_length_3211_cov_3.737009, whole genome shotgun sequence.
ACCESSION   DAOSPL010000396 DAOSPL010000000
VERSION     DAOSPL010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30065_length_3211_cov_3.737009"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="PLQ54_08380"
     CDS             complement(<1..1459)
                     /locus_tag="PLQ54_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93314.1"
                     /translation="MHDHVMFVGDQRLTFGLSLGGGRLRERDYRVSGTEMAVSLGEPW
                     VVEADGEALTADHGPARVLRDNATEARFVGENHAFGWELAYQLLGAGRVTKTLTLTPR
                     RRVRIERVWLWSAPSPEPPGVARTGLQEIAAFYRQGADGLFVSLDFPWSRITATDGEA
                     RVGYVPFDPVAAGEVYTCHSLTVGATRVTGRVRHGFHEGEVDAVDAYVQERFAPRFNR
                     PLFSTACINNRYTQVEGDWVFYSMRDHPTLTMNRELLRGELRLSARLGLEYYQVFPGV
                     FDWGPDDPSPEMVREAVEWAHDEGIRMGDYSGANVVFCGHYNQHRNRLDRPEWRIIDA
                     NGNPGAFCLGHPDFVDHYAGVVARTCRRFGFELHALDFLSIAPCHADDHGHPPGPDSV
                     YHQVRGLVRFMDEVNAVSPEMLIWPNSGNWSEFLPKLAWWAPSLYLTDPFVATPWQGL
                     NQTRILDDCRREQMVGLHHSHFVPYRFFTNCQYFFS"
     gene            complement(1755..>3211)
                     /gene="feoB"
                     /locus_tag="PLQ54_08385"
     CDS             complement(1755..>3211)
                     /gene="feoB"
                     /locus_tag="PLQ54_08385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOS93315.1"
                     /translation="PRIRVRSSPRRLVWLAMSLLVGEEPAWPGLAADDPALARVRSTA
                     RAAARHLEEVTGDTAEAVITGGRYGAIQGVYREAVDEPREAGPTPSDRIDRVLLHRVW
                     ALPILLATVAAMFELTYVVGEYPSGWIGEGVAALGGCVSAWMPDGPLRSLLVDGVIGG
                     VGGVLVFVPQIFILFAIIALLEDSGYMARAAFVMDRVMHRMRLHGKAFIPMVMGFGCN
                     VPAIMATRTLESPRDRLVTILVAPLMTCSARLPVYALIAGALFGPHAGLAIFSMYVLG
                     VVLAALVARILRHHVLRGEEEPFVMELPPYHRPTAKGIAIHTWERGKLFLQKAGTVIL
                     AGSVIMWCLSALPWGVEVAGPQSYAGQLGKALEPAVRPLGLEWQEAVALVSGFVAKEI
                     VVSTMGVLYGVRAEAGDAELGRAIASSHLTPLSGYALMAFVLVYSPCIAAIAAMRRET
                     GSWKWAIIAVAYLTALAWVVAFVIFQGGRLLGLG"
BASE COUNT          572 a         1155 c         1027 g          457 t
ORIGIN      
        1 ggctgaagaa gtactggcag ttcgtgaaga agcggtatgg gacgaagtgc gagtgatgta
       61 ggccgaccat ctgctcgcgg cggcagtcat cgaggatccg cgtctggttc agcccctgcc
      121 agggcgtcgc gacgaacggg tcggtcagat agagcgacgg cgcccaccag gccagcttcg
      181 gcaggaactc ggaccagttg cccgagttcg gccagatgag catctcggga gagacagcat
      241 tcacctcgtc catgaaccga accagcccgc gaacctggtg atagacgctg tccggccccg
      301 gcgggtggcc gtgatcgtcc gcgtggcatg gcgcgatgct aaggaagtcc agggcgtgca
      361 gctcgaagcc gaaccggcgg cacgtgcgcg cgacgacacc cgcgtagtgg tcgacgaagt
      421 ccggatggcc caggcagaaa gctcccgggt tgccattggc gtcgatgatc cgccactcgg
      481 gccgatccag ccggttccgg tgctgattgt agtggccaca gaagaccacg ttggcgcccg
      541 aatagtcgcc catgcggata ccctcgtcgt gggcccactc gacggcctcc cgaaccatct
      601 ccgggctggg gtcatccggt ccccagtcga acacacccgg gaacacctgg tagtactcga
      661 gccccagccg cgccgacaac cgcaactcgc cccggagcag ctcgcggttc atcgtgagtg
      721 tcgggtggtc gcgcatgctg tagaacaccc agtcgccttc gacctgcgtg taccggttgt
      781 tgatgcaggc ggtgctgaag agaggacggt tgaagcgggg agcgaagcgc tcctggacgt
      841 acgcatccac ggcgtccact tcgccctcgt ggaacccgtg gcgaacgcgg ccggtcacgc
      901 gcgtcgcgcc gacggtcagc gagtggcacg tgtagacctc gcccgcggcg accgggtcga
      961 acggcacgta gccaacccgg gcctcgccgt cggtggcggt gatccgcgac catgggaagt
     1021 ccagcgagac gaacagccca tcggcaccct ggcggtagaa ggccgcgatc tcctgcagcc
     1081 cggtgcgggc gacccctggc ggctcgggcg agggggcgct ccacagccag acgcgctcga
     1141 tccgaacccg ccgcctcggt gtgagggtga gcgtcttggt gacgcgcccg gcgcccagga
     1201 gctggtacgc caactcccag ccgaatgcat ggttctcgcc gacgaaccgg gcctccgtgg
     1261 cgttgtcgcg cagcacgcgc gccggcccat ggtccgccgt aagcgcctcg ccgtcggcct
     1321 cgaccaccca cggctctccc aacgagacgg ccatctcagt ccccgagacg cggtagtcgc
     1381 gctcccgcag tcggccgccc ccaagagaca gcccgaacgt gagccgctgg tcgcccacga
     1441 acatgacgtg gtcatgcatg gagacccctc cgcctctatg gctccgcctc gtgcacccgt
     1501 ggcgccacgg agttacacag acccgccggc tcgtcggctc cggctgtcca accctcgagc
     1561 ccgcactcga ggtccggatg ctcacggcca gatcggcaac tggcggtcct cgctgtcctt
     1621 cgggagccag gggtggcgac gcatgccacg gtacgggctg gggatcggcc gcgtcgccgt
     1681 cgggtaacgc tgcccggtgg ctggcgcgcc atcatgacga acaccggtgg tgtccgcaac
     1741 tacgccgtcg catgctagcc cagcccgagc aggcggcccc cctggaagat cacgaaggcg
     1801 accacccagg ctagagccgt gaggtaggcc accgcgatga tcgcccactt ccacgaaccc
     1861 gtctcccgcc tcatggcggc aatcgccgcg atgcagggcg agtagaccag cacgaacgcc
     1921 atcagggcat agcccgacag cggcgtcagg tggctgctcg cgatagcccg gccgagttcc
     1981 gcatcgcccg cctcggccct gacgccatac agcacgccca tggtcgagac cacgatctcc
     2041 ttcgccacga agcccgacac cagggccacg gcctcctgcc actcgaggcc caggggcctc
     2101 acggccggct ccagcgcttt cccaagctgc cccgcgtaac tctgggggcc cgccacttcc
     2161 accccccaag gcaacgcact gaggcaccac atgatcactg agcccgccag gatcacagtg
     2221 cccgccttct gcaagaagag cttcccacgt tcccatgtgt gaatggcaat ccccttggcc
     2281 gttggcctgt ggtacggagg aagctccatc acgaatggtt cttcctcgcc ccggagcaca
     2341 tgatgccgca ggatccgtgc caccagcgcg gccagcacca cgccgagcac gtacatcgag
     2401 aagatcgcca gtccggcatg ggggccgaac agggctccgg cgatcaatgc atagaccggc
     2461 agtcgggcgc tgcaggtcat caagggagcc accaggatcg tcaccaggcg gtcgcgcggc
     2521 gattcgagtg tccgggtcgc catgatggcc ggcacgttgc acccgaagcc catcaccatc
     2581 gggatgaacg ccttcccgtg caggcgcatc cggtgcatca ctcggtccat cacgaaggcc
     2641 gcccgcgcca tgtacccgct gtcttcgagc agcgcaatga tggcgaacag gatgaagatc
     2701 tggggaacga acaccaggac accgcccacg ccgccgatca cgccatccac cagaagcgat
     2761 cggaggggcc cgtctggcat ccaggccgag acacagcctc cgagcgcggc cacgccctcc
     2821 ccaatccagc cgctggggta ctccccaacg acataggtga gctcgaacat tgccgccacg
     2881 gtcgccagga ggatcggcag ggcccagacg cggtgcagta ggacccggtc aatgcggtct
     2941 gacggcgtgg gaccggcctc gcgcggctca tccaccgcct cgcggtacac tccctggatc
     3001 gcgccgtagc ggcctccggt gatcacggcc tcggccgtgt cgccggtcac ttcctccaaa
     3061 tgccgggccg ccgcccgggc cgtggaccgc acccgcgcga gggctgggtc gtctgcagcc
     3121 agccctggcc acgcgggctc ctcacccaca agcaagctca tcgcaagcca caccagccgc
     3181 cgaggtgacg accgaactcg aatccgtggt c
//
LOCUS       DAOSPL010000397         3206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_30110_length_3206_cov_3.106315, whole genome shotgun sequence.
ACCESSION   DAOSPL010000397 DAOSPL010000000
VERSION     DAOSPL010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30110_length_3206_cov_3.106315"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLQ54_08390"
     CDS             <1..324
                     /locus_tag="PLQ54_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93316.1"
                     /translation="RTQCGLCGGDCVAQPELSDEVVGPHRVGRRVMSVVADLRTQCPV
                     PFLNAREEASPDDPAVAPWRSEIRTLRDHARAYQEEQAALEAPIAMRALTDRPRVCVR
                     SNGLR"
     gene            321..653
                     /locus_tag="PLQ54_08395"
     CDS             321..653
                     /locus_tag="PLQ54_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93317.1"
                     /translation="MKLARPRLCRKRGPCRALAARVRMRKFLQEPFVFVERPKGPSEN
                     QAAELAIRPLGTARKVFGATRSARGSQTMAVLQTLFGTWSLPAVSIPSMPAWLCLPPP
                     EATHPHRL"
     gene            852..>3206
                     /locus_tag="PLQ54_08400"
     CDS             852..>3206
                     /locus_tag="PLQ54_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93318.1"
                     /translation="MPSHDRTAGILGLLRGLNGLDPLKRLFWTELNYDRINQPIPRRQ
                     WTENQRALLAEDPILFAGAGDGHGFQIIYSKLDSDRLLLTPERTIVSKLQPEHPYALF
                     IFSNRDQDRWHFVNVKQQARDAAPQREGSRKAPQLFRRITVGPEERLRTASQRVAMLD
                     VATIPAGPFAVSHLDIQARHDEAFDVEAVSKQFFAEYQTVFRSLQADLQKQTRDAVWA
                     HDYALQFLNRLMFLYFVQRKRWLGEDTEFLRAFWEAYQDAKQPRNSFFAQWLSVLFFE
                     AFNNRFHGGHRQFPDDIRNALQMAPYLNGGLFRENRLDTSPPGAFTISDERFEEAYRF
                     LESYNFTIAEDSPLDQEVAVDPEMIGKVYESLVNVSSEADERGDAGIFYTPRTEIDLM
                     CRLALVDNLANHLGQQHKNLLYEVVFALEPEDKETADCRVQKAGLWDHLNDRLRRTTV
                     VDPACGSGSFLVGMLHVLDDLQARAQSRLGIHEDSYDRKKRIIGQSLYGVDVMEWACH
                     VAELRLWLALIIDADIDTTAAHVRREPLLPHFSFKIRCGDSLVQEVGGLNMAHIKGSS
                     LVPPPVKRKITIHKERKLQFYNNDEDREYKTEEQVKHAELRLFREILDAQDKAAEQQV
                     VQLNQRIANPKARQIRLDGTVETTPQMALEAAELRRQVVELEGRRKWLQETRQALQPV
                     HDVPFVWDIAFVEVFEGDSDGFDIVVGNPPYVRQEKIADPRERQEDFTADEWRERRKA
                     YKAKLARSVYQAYPGFFRYDPAGDAAANKIDSKSDLYVYFYLHAL"
BASE COUNT          572 a         1076 c         1050 g          508 t
ORIGIN      
        1 cggacacagt gcgggctgtg cggcggggac tgcgtggccc agccggagct gtctgatgag
       61 gtcgtggggc cgcaccgagt gggccgtcgg gtcatgagcg tggtggccga ccttcggacc
      121 cagtgcccgg tgccatttct gaatgcgcgg gaggaggcgt ccccggacga cccggcggtc
      181 gcgccgtggc gatcggagat ccggacgctg cgcgatcacg ccagagcgta tcaggaggaa
      241 caggcggctt tggaggcgcc catcgcgatg cgcgcgttga cggaccggcc tcgggtgtgc
      301 gtacgttcga acgggctccg atgaagctgg cccggccgcg tctgtgccgg aagcgaggtc
      361 cgtgccgggc gttggcggcg cgagtgcgga tgcggaagtt cctgcaggag ccgttcgtct
      421 tcgtggagcg ccccaaaggc ccgtcggaga accaggcggc ggagttggcg atccggccgt
      481 tgggtaccgc gcggaaggtg ttcggggcga cgcgcagcgc ccgcgggtcg cagacgatgg
      541 cggtcctgca gaccctgttc gggacgtggt cattacccgc ggtctcgatc ccctcgatgc
      601 ctgcatggct atgcttgcct cctcctgagg ccacacaccc acaccgactc tgagctctca
      661 cacctgtgaa gttaggaagg acacgggaca cacgacggcg aagaagatag tgccgtgagc
      721 cctggcacgg gtgggactca gctggcagcc ttcacccgcg aacctcggtc acgctgttcc
      781 tgcgtcctcg gcgccatcgg cggtcgcccg ctcgaatggt tgcgttggct cgcggtctgg
      841 agggcgggct catgccgagt cacgaccgca ccgctggcat cctggggctc ctcagggggc
      901 tgaacggcct cgatcccctc aagcggctct tctggacaga gctgaactac gaccgcatca
      961 accagcccat cccgcggcgg cagtggaccg agaaccagcg ggccctgctc gccgaggacc
     1021 ccatcctctt cgccggggcc ggtgacggcc acggcttcca gatcatctac tcgaagctgg
     1081 actcggaccg tctcctgctg acccccgaac gtaccatcgt ctcgaagctc cagcccgagc
     1141 atccctacgc cctcttcatc ttctccaatc gcgaccagga ccgctggcac ttcgtcaatg
     1201 tgaagcagca ggccagggac gccgctcccc agagagaggg ttcccgcaag gccccgcagc
     1261 tcttccgccg catcacggtg ggcccggagg agcggctgcg gacggcatcc cagcgtgtcg
     1321 cgatgctcga tgtcgcaacc attccggccg ggccgttcgc cgtgtcccac ctcgacatcc
     1381 aggcccggca cgacgaggcg ttcgacgtcg aggccgtcag caagcagttc ttcgccgagt
     1441 accagaccgt gttccggtcg ctgcaggccg acctccagaa gcagacgcgg gatgcagtct
     1501 gggcccatga ctacgccctg cagtttctca accgcctgat gttcctctac ttcgtccagc
     1561 ggaagcgttg gctgggcgag gacaccgagt tcctgcgggc cttctgggaa gcctaccagg
     1621 atgccaagca gcccaggaac agcttcttcg ctcaatggct ctcggtcctg ttcttcgaag
     1681 cgttcaacaa tcgcttccac ggcggacacc ggcagttccc ggacgacatc cgcaatgccc
     1741 tgcagatggc tccctacctc aacggcggcc tgttcaggga gaaccgtctc gacacgagcc
     1801 cgccgggggc cttcacgatc tcggacgagc ggttcgagga ggcctaccgg ttcctcgaga
     1861 gctacaactt caccatcgcc gaggacagcc cgctcgacca ggaggtcgcc gtcgatcccg
     1921 agatgatcgg gaaggtctac gagagcctgg tcaacgtctc ctccgaggcg gacgagcggg
     1981 gcgatgcggg catcttctac acgccccgca ccgagattga cctcatgtgc cgcctggccc
     2041 tggtggacaa cctcgccaac cacctcgggc agcagcacaa gaacctgctc tacgaggtcg
     2101 tcttcgccct ggagcccgaa gataaggaaa cggcggactg ccgggtgcag aaggcgggcc
     2161 tgtgggatca cctcaacgac cggctgcgga ggaccaccgt cgtggacccc gcctgcggct
     2221 ccggctcctt cctggtgggg atgctgcacg tcctcgacga cctgcaggcc cgggcccaga
     2281 gccgcctcgg gatccacgag gactcctacg accgcaagaa gcggatcatc ggccagagcc
     2341 tctacggcgt ggatgtgatg gagtgggcct gccacgtcgc cgagctgcgg ctctggctgg
     2401 ccctcatcat cgacgccgac atcgacacca cagcggccca cgttcggcgg gagccgctgc
     2461 tcccgcactt cagcttcaag attcgctgcg gcgacagcct cgtgcaggag gtcggtgggc
     2521 tgaacatggc ccacatcaag ggctcctcgc tcgtgccccc gccggtcaag cggaagatca
     2581 ccatccacaa ggagcggaag ctgcagttct acaacaacga tgaggaccgt gagtacaaga
     2641 ccgaggagca ggtcaaacac gcggagctgc ggctcttccg cgagatactc gacgcccagg
     2701 acaaggcggc agagcagcag gtcgtccagc tcaaccagcg gatcgccaac ccgaaggcga
     2761 ggcagattag gctcgacggc accgtggaga cgacgcccca gatggcgttg gaagcggcgg
     2821 agctgcggcg tcaggtggtg gagctggagg gtcgccgcaa gtggctgcag gagacccgcc
     2881 aggctctcca gccggtccac gatgtgccct tcgtctggga catcgccttc gtggaggtct
     2941 tcgagggcga cagcgatggg ttcgacatcg tggtcggcaa cccgccatac gtgagacagg
     3001 agaagatcgc cgaccccagg gagcgacagg aggacttcac agccgacgag tggcgagagc
     3061 ggaggaaagc ctacaaggcc aagctggccc gctcggtcta ccaggcgtac cccggcttct
     3121 tccgttatga cccggccgga gacgccgccg ccaacaagat cgactccaag agcgacctgt
     3181 acgtctactt ctacctgcac gccctg
//
LOCUS       DAOSPL010000398         3203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_30143_length_3203_cov_3.308450, whole genome shotgun sequence.
ACCESSION   DAOSPL010000398 DAOSPL010000000
VERSION     DAOSPL010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30143_length_3203_cov_3.308450"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2603)
                     /locus_tag="PLQ54_08405"
     CDS             complement(<1..2603)
                     /locus_tag="PLQ54_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93319.1"
                     /translation="MRTAGWAVVAAIVTASVAGAARTGPDAPLIAVLREAGLPSAARA
                     DGLLRAFRHGGMRAEPISYAEACDPAVLSAARVRLLVVPDSPHFPAAAAEALLRYLRS
                     GGHVALLGGRAFSDPAVWVGRQWLSQSDLARRRAETPVQHMLFDFEPGDAAQWQRNTN
                     KPEPASRALDVEGPHGDALLLELNGMGPWQWDAFGADLAAAPPPGHNVVCLWAKGGPT
                     TRQAALEIDERDGSRWVATVDTPEQWTRQVVRAADFRFLADGSPAGRGGDGDSLRLDE
                     GTRLSLGLATGLTNHPDGDHRVWVAEIGTATSDLPDPPGVELPLDAFSDYEPYVLRDV
                     TGVRPWAGQAIVRCSPAGRCAASGLSAVGFALARKSEFSPLLECVDEYGRGRGWALGM
                     LTHYEGDYRGGSWLLSGVTTPEFYETPAFQAAIVESATALLSRDLASEARQRDEASRA
                     TQIRLTTPAPHAFVRLSADRRRFVTPDGKPLFLTGCNYANHFDRQFHWGWAFDAARVE
                     ADFRRAKAAGHNAFRLWSGALGGPDEVEAIRELARRYGIYLIIHCGAGGTMEQITESV
                     RSTAEAWRDEPMVIGYDLMNEPYVETVGAITSGGRLSPIVELRPSARFAGMLDPSWVE
                     QCVRQPPSWPPMPGGLSDDVRRELYVAGLLWGRVISGRTAGGADYSTLTGADGLIRAQ
                     GDLADLVTAVDRTFARWIGAQVDAIRSVDSNHLITVGYNTALACLPANGTLDFVCHHI
                     YQPPTSYQDIVKNATALDRLATVWPDRPVMLGEFGYSNGVRLGESCLDWHASSVGEMT
                     VYLAAMAHGHAGALKWLLDEWPEAVMRDNAGWMSPDNAYEQRFGSFAYDGTPEMKPKP
                     IAYALRFLREY"
     gene            complement(2630..>3203)
                     /locus_tag="PLQ54_08410"
     CDS             complement(2630..>3203)
                     /locus_tag="PLQ54_08410"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOS93320.1"
                     /translation="PAGAEGVDDDRRLAEFSGADLRQSFTPEELAVLKRAWSPRPPEP
                     TQTPADIPAVVTSVGLTEAKWEAAQVGWQAPARDLLEGGFPLRSSERVHERGLYAHAP
                     SRYVFDLGGGWGQLDAFYGLQSGNTGSVVFVVMGDGAELFRSERVTDAVERAVQVDVT
                     GVRMLELVVEDGGDGRSGDWGIWFSPVLSR"
BASE COUNT          526 a         1143 c         1098 g          436 t
ORIGIN      
        1 atgtactctc ggaggaagcg caaggcgtac gcgatgggct tgggcttcat ctccggcgtc
       61 ccgtcgtacg cgaacgaccc aaaccgctgc tcataggcgt tatcgggcga catccacccc
      121 gcgttgtccc gcatgacagc ctccggccac tcgtcgagca gccacttgag cgcgccggcg
      181 tgcccgtgag ccatggcggc caggtacacg gtcatctcgc ccaccgacga ggcgtgccag
      241 tcgaggcagc tctcgccgag ccggacacca ttcgagtacc cgaactcgcc cagcatcacg
      301 ggccggtccg gccacacggt ggccagtcgg tccaaggccg tggcgttctt gacaatgtcc
      361 tggtaggagg tcggcggctg gtagatgtgg tgacagacga agtccagtgt gccgttcgcc
      421 ggaaggcagg cgagggcggt attgtagccg accgtgatga ggtggttcga gtcgacagac
      481 cggatggcat cgacctgcgc gccgatccac cgggcgaagg tgcgatcaac cgcggtcacc
      541 agatcagcaa gatccccctg ggcccggatg aggccatccg caccggtgag ggtcgagtag
      601 tcggcgccgc cggcggtgcg cccgctgatc acgcggcccc agagcagccc ggcgacgtag
      661 agctcgcgcc gcacgtcgtc cgacagcccg ccaggcatcg gcggccagga gggcggttgc
      721 cgaacgcact gctcgaccca gctcgggtcg agcatgcctg cgaaccgggc cgacgggcgg
      781 agctcgacga tggggctcag ccgtccgccg gaggtgatgg cgccgacggt ctcgacgtag
      841 ggctcgttca tcaggtcata gccgatgacc atcggctcat cgcgccacgc ctcggcggtg
      901 gaccgcacgc tctccgtgat ctgctccatc gtgccgccgg cgccgcagtg gatgatcagg
      961 tagatgccgt agcgccgggc cagctcgcgg atagcctcga cctcatccgg acctccgagc
     1021 gcaccgctcc agagccggaa ggcgttgtgg ccggcggcct tggcccggcg gaagtcggcc
     1081 tcgacccggg ccgcgtcgaa ggcccagccc cagtggaact ggcggtcgaa gtggttcgcg
     1141 tagttgcagc ccgtcaggaa cagcggcttc ccgtcaggag tgacgaagcg acggcggtcg
     1201 gcgctgaggc ggacgaacgc gtgtggtgca ggggtggtca gtctgatctg cgtggcgcgg
     1261 ctggcctcgt cgcgctggcg cgcctcggaa gcgaggtcgc gcgagagcag tgccgtcgct
     1321 gactccacga tagccgcctg gaaagcgggg gtctcataga actccggcgt cgtgacgccg
     1381 ctgagcagcc aggatccgcc gcggtagtcg ccctcgtagt gagtgagcat gcccagcgcc
     1441 cagccacgcc ctcggccgta ctcgtcgacg cactcgagaa ggggactgaa ctcggacttc
     1501 cgggccaggg cgaagccgac ggccgacagg ccgctggcgg cacaacgacc ggcagggctg
     1561 cagcgcacga tggcctgccc tgcccatggg cgcacgccgg tcacgtcacg cagcacgtag
     1621 ggctcgtagt cgctgaaggc gtccagcggt agctcgaccc cgggcggatc gggcagatcg
     1681 ctcgtcgccg tgccgatctc ggcaacccac acccggtggt cgccatcggg gtgattcgtc
     1741 agtccggtgg cgaggcccaa tgacagccgc gtaccctcgt ccaggcgcag cgaatcgccg
     1801 tcgccaccgc gcccagcggg cgagccgtca gcgaggaagc ggaagtcggc ggcccggacc
     1861 acctgacggg tccattgctc gggcgtatcc actgtggcaa cccaccgcga gccatcgcgt
     1921 tcgtcgatct cgagcgcggc ctgccgcgtg gtcggtccgc ccttcgccca caagcacacg
     1981 acgttgtggc ccggcggcgg cgccgccgcc aggtcggccc cgaaggcgtc ccactgccac
     2041 gggcccatgc cgttgagctc cagcaggagc gcatcgccgt gcgggccctc aacatcgagc
     2101 gcgcgcgaag cgggttctgg cttgttcgta ttccgctgcc actgcgcggc gtcgcccggt
     2161 tcgaagtcga agagcatgtg ctggactggc gtctcggcgc gcctccgcgc gagatcggac
     2221 tgcgagagcc actgacggcc gacccaaacg gctggatccg agaatgccct cccgccaagc
     2281 aaggcgacgt ggccgcccga gcggaggtac cgaagcaacg cctctgctgc ggctgccggg
     2341 aagtgcgggc tatcgggaac gaccaggagc cggaccctgg ccgcggacag cacggccggg
     2401 tcgcaggcct ccgcgtagct gatgggctcg gcccgcatgc cgccatgccg gaacgccctc
     2461 agcaacccgt cggcccgggc cgcagagggg aggccggcct cgcgaaggac cgcgatcagg
     2521 ggggcatcgg gcccagtgcg cgccgcgccc gcgaccgatg cggtcacgat agccgccaca
     2581 accgcccacc ccgctgttcg cataccgagc ccctctgctc gcgacgggcc taccggctca
     2641 gtacggggct gaaccaaatg ccccagtcgc cgctacggcc atcgccgcca tcctcgacga
     2701 cgagctcgag catccgcacg ccggtcacgt ccacctgcac ggcccgctca acggcatcgg
     2761 tcacgcgttc ggagcggaac agctcggcac catcgcccat gacgacgaac acaaccgaac
     2821 ccgtgttacc gctctggaga ccgtagaacg cgtcgagctg gccccacccg ccgcccagat
     2881 cgaacacata gcgcgacggc gcgtgagcat acaaccctcg ctcgtgtacc cgttcggacg
     2941 accgcagcgg gaagccaccc tcaagcaaat cccgcgccgg cgcctgccat cccacctggg
     3001 ccgcctccca cttggcttcg gtcagaccca cggacgtgac aacggccggg atgtcagcag
     3061 gagtctgcgt gggctcggga ggacgcgggg accaggcgcg cttcagcacg gccagctctt
     3121 cgggcgtgaa cgactggcgc aggtcggcac ccgagaactc ggccagccgg cggtcgtcgt
     3181 ccacaccctc ggcacccgcc ggg
//
LOCUS       DAOSPL010000399         3203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_30145_length_3203_cov_2.515565, whole genome shotgun sequence.
ACCESSION   DAOSPL010000399 DAOSPL010000000
VERSION     DAOSPL010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30145_length_3203_cov_2.515565"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PLQ54_08415"
     CDS             complement(<1..271)
                     /locus_tag="PLQ54_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOS93321.1"
                     /translation="MGDALAIVLGLICCLGLAIVGGIILMYNGLITLRGRAENAWSQI
                     DVQLRRRYDLIPNLVETVKGYAKHESQTLEAVIAARNTAMNATGVG"
     gene            complement(335..1426)
                     /locus_tag="PLQ54_08420"
     CDS             complement(335..1426)
                     /locus_tag="PLQ54_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOS93322.1"
                     /translation="MAETMLAAVLEGPRQLVVRPVPRPRCGPGEVLVRMAACGVCGSD
                     LRYFEGENPWAIHTLGIDKPNRPGMILGHEVSGTIVEVGDGVSPDRIGERVVAEAFRT
                     CGSCPDCLRGRANLCANTEHLGHGAGWEGAEFNPGGMAETFTIWADKALPLPDAVSFD
                     EAAFLDGLGVAVHAVRRAGVREGDAVLIWGAGPIGLCLAQVARARGARLVACVDVYSV
                     ALRLAEELGADVVADGRCAEPLTELMERTSGRGADAAFDTTGHLAAQQGALAALAPGG
                     TCLLMAGPAPGLQVGLRGLAGERTLTTAANFAEGDLAEAVRLVESGQVRVRPMITHRV
                     PLARAPEAFELMRQKEHRGTVKVLIVPDG"
     gene            complement(1464..2204)
                     /locus_tag="PLQ54_08425"
     CDS             complement(1464..2204)
                     /locus_tag="PLQ54_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS93323.1"
                     /translation="MGSICISASLEVFPDPPYFDIWSVEQVRCVAAALDETAIRIYAI
                     HVPFGDAVDLSSASTEAVENGLRHAKRVIDLCQQFEARFAVIHPGHILGEGADEAGRY
                     RQATRSLKALVSRADSAGVVLAVENLPPDHVGSDLDRVVDLVAEVGSPAVGVCFDTGH
                     ANLYSPPQRPCAAGKHLVWIHAYDNRGQRDEHLMPGLGTVDWSQFRADLDAVGYSNPI
                     MLECRPAEDMTVQGMIDRFFELTGYEPS"
     gene            complement(2378..2785)
                     /locus_tag="PLQ54_08430"
     CDS             complement(2378..2785)
                     /locus_tag="PLQ54_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93324.1"
                     /translation="MRRFAPVVLLITSGLIVLGCDRVNPNTAAPAARPTVPVARPPEP
                     ADPVREAPLEDQAPPTATRRGPTLRPRESFALDAARALKPDYPGRAIEWHSPDWREVI
                     VALGTTEDNLDLRVRLRWDDVWGYRYVGEERGF"
     gene            2918..>3203
                     /locus_tag="PLQ54_08435"
     CDS             2918..>3203
                     /locus_tag="PLQ54_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93325.1"
                     /translation="MTNKERVYTAFAHEQPDRTPYMIGFTQKAHSAMVDRYGPDYADA
                     IDNCFHGVHADPGPKDHWLDENTWQDEFGVRWDRRIDRDIGNVCNQVLPER"
BASE COUNT          523 a         1122 c         1075 g          483 t
ORIGIN      
        1 cgcccacgcc tgtggcgttc attgcggtgt tccgcgcggc gatgaccgct tcgagcgtct
       61 ggctctcgtg cttcgcgtag cctttgaccg tctcgaccag gttcgggatc aggtcatagc
      121 gccggcgaag ctgcacgtcg atctgtgacc atgcgttctc cgcccggccc cgcagtgtga
      181 taaggccgtt gtacatcagg atgatgccgc ccacgatcgc caggccgaga cagcagatca
      241 agcccagcac gatggccaga gcgtccccca tcgtataccc tccctcgtcg cgatcaccgt
      301 gcccgaagcg gggcggaccc actccgggag ctggctaccc atccgggaca atcagcacct
      361 tcactgtccc gcggtgttcc ttctgccgca tcagctcgaa tgcctcgggg gcgcgtgcca
      421 gcggcactcg gtgcgtgatc atcggccgca ccctcacctg cccgctctcg accaagcgca
      481 ccgcctcagc gagatcaccc tcggcgaagt tcgcggccgt ggtgagcgtc cgctctcccg
      541 ccagccctcg cagccccacc tggagcccgg gcgccgggcc agccatcaga aggcatgtcc
      601 cgcccggtgc cagcgcggcc agcgcgccct gctgcgcagc caggtggccg gtggtgtcga
      661 acgcggcatc ggctccccgg ccgctcgtcc gttccatcag ctcggtcagg ggctccgcac
      721 accggccgtc ggccaccacg tccgccccga gctcctcggc cagacgcaac gccactgagt
      781 agacgtccac gcacgccacg agtcgcgcac ctcttgcgcg agcgacctgg gcgaggcaca
      841 gcccgatcgg tcccgcgccc cagatgagca ccgcgtcgcc ctcgcgcacc ccggcgcgac
      901 ggacggcgtg gaccgcgacg cccaggccat cgaggaaggc ggcctcgtcg aaggagacgg
      961 catcgggcag cgggagcgcc ttgtccgccc agatggtgaa ggtctcggcc atgccgcccg
     1021 ggttgaactc ggcaccctcc cacccggccc cgtgcccgag atgctcggtg ttggcacaca
     1081 ggttcgcccg gccccggagg caatcaggac agcttccgca ggtgcggaac gcctcggcga
     1141 cgacccgctc gccgatgcgg tcgggcgaga cgccgtcacc cacctccacg atggtcccgc
     1201 tgacttcgtg gccaaggatc atgccgggcc ggttcggctt gtcaatgcca agcgtatgga
     1261 tggcccaggg gttctcgccc tcgaagtagc gcaggtcact gccgcagacg ccacaggcgg
     1321 ccatccggac gagcacctcg cccgggccac agcgcggccg cgggacgggg cgcaccacca
     1381 actggcgcgg gccttcgagc acggctgcga gcatggtctc ggccatcggg gtcacctcgg
     1441 gcgcgggtgg tcgtgtcccg agcctagctc ggctcgtacc ccgtgagttc gaagaagcgg
     1501 tcgatcatgc cttgtacggt catgtcttcg gcgggccggc actcgagcat gatggggttc
     1561 gaatagccca ccgcgtcgag atccgctcgg aactgggacc agtcgacggt tccgagaccc
     1621 ggcatgaggt gttcgtccct ctgtccccgg ttgtcgtagg cgtggatcca gaccaggtgc
     1681 ttcccggccg cgcacggccg ctggggagga gagtacaggt tcgcgtggcc cgtatcgaaa
     1741 cacacaccca cggcgggact gccgacctcg gccacgagat cgaccacgcg gtcgagatcg
     1801 gatccgacat ggtcgggcgg cagattctcg acggccagaa caaccccagc gctgtccgct
     1861 cgggagacca gcgccttgag tgaccgtgtg gcctggcggt agcggccggc ctcgtctgcg
     1921 ccctcgccca ggatgtggcc gggatggatc accgcgaagc gggcctcaaa ctgctgacag
     1981 agatcgatga cccgcttggc gtgccggaga ccgttctcga cggcctcggt cgaggcagag
     2041 gacagatcga ccgcgtcgcc aaagggcacg tgaatggcat agatgcggat ggccgtctcg
     2101 tccagcgcgg cggccacgca gcggacctgc tccaccgacc agatgtcgaa gtagggcgga
     2161 tctgggaaga cctcgagcga cgcgctgata cagatgctac ccatatcccg aagactctca
     2221 ggtagcgtgt tagcgccgaa aacggttgga gggaccacga tatccaccgg gcggacagga
     2281 cctcgtctcc aggtgcagtg ggccgagtcg ggagacgctc ctggccccat tccatgatac
     2341 cgcaatgcgt gcggggcgtc agcgtgggcg gcggcagtca gaaaccccgc tcctcgccga
     2401 cgtagcggta gccccagacg tcatcccacc ggagccggac gcggaggtcg aggttgtcct
     2461 ctgtggtgcc gagggcgacg atgacctcgc gccagtcggg gctgtgccac tcaatggccc
     2521 tgccggggta gtcgggcttg agggcgcgcg cggcatcgag ggcgaaggac tcgcgcggac
     2581 gcagtgtggg cccacggcgg gtggccgtcg gaggagcctg gtcctcgagg ggcgcctcgc
     2641 gcacgggatc ggcaggctct gggggtctgg caactggcac cgtcggccgg gccgccgggg
     2701 ccgcggtgtt gggattcact cgatcgcagc ccagcacgat gaggccgctg gtgatcagca
     2761 gcacaacggg tgcgaagcgc cgcacggtaa gccctccact tcggctactc ctcctcttcc
     2821 gtggccgcat tgtaccaggt ccgcccgcgc aggttcggag ggtggcaccg acgaaccggc
     2881 cacccagtgc tcacttcccg cgctgggggc gtcacgcatg acgaacaagg aacgggtcta
     2941 caccgcgttc gcccacgaac agcctgaccg gacgccctac atgatcgggt tcacgcagaa
     3001 ggcccattcg gccatggtcg accgctacgg tcccgactat gcggacgcca tcgataactg
     3061 cttccacggc gtccacgccg atcccgggcc caaggaccac tggctcgacg agaacacctg
     3121 gcaggatgag tttggcgtgc ggtgggaccg gcgtatcgat cgggacatcg gcaacgtctg
     3181 caaccaggtg ctaccggagc gcg
//
LOCUS       DAOSPL010000400         3200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_30179_length_3200_cov_2.925278, whole genome shotgun sequence.
ACCESSION   DAOSPL010000400 DAOSPL010000000
VERSION     DAOSPL010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30179_length_3200_cov_2.925278"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PLQ54_08440"
     CDS             complement(<1..449)
                     /locus_tag="PLQ54_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93326.1"
                     /translation="MRPSRVLVLALAALGMMAVPGSARIRYMNFQPIEKPIYRAPSAL
                     PAQPNPATVTDRPAWVPVSDPSAGANPYTGVAGGNTLPALIHDWVTLDVDRTGGAEGT
                     YVFRWGDLRRIKIDVDLDLVWGTVNTTDPGSPAYTPGSAADLDGDGTR"
     gene            complement(559..>3200)
                     /locus_tag="PLQ54_08445"
     CDS             complement(559..>3200)
                     /locus_tag="PLQ54_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93327.1"
                     /translation="SGPITRPSYSGGQIADADDVDDPLEFDTNDADNNPGIVPFDIDF
                     ETGIVSFTIDALGASDTTSGIPGDIEPVRDADMDGVSADPSPDYVVSAIGSPTISSDK
                     AIVPVTYSHAPDPLTNTRSEALLVFAMDPRDSVYYDEANEPWPGASVPWDTTLSPPWP
                     SYRYIDDGEANGWVSYWTNQVGALGQPHNPSYDLLSVVNGLTSGEGIASTQAVVGRGR
                     IYTVSVAPRAAAANQWNWFYRVYEAGTSGLVIGDLNRVLSVDAAGSPSWEVASARYLY
                     VSDPTGNAADIVSLPVRISRPQSVLALDYAGKPNNYLVADSGNDRVIELRKDGVADWM
                     LQNRWDPNHLGSGQGAEAAFRDTPDYAPPDGGPAYPNLPPGASHQIREPHDAYRWAWY
                     DYSGPEPVLREWTFVADTGNFRIIAVEQLTNEDGDRLPPLDESGGEAIYAYDQTQAVI
                     DPTAAGEEVLPVDLDGDGTPDTGAQGQLKTAGKYEFITVKPLMNPGLFGGPDNPMGFS
                     GVLAAVANYALSTSPIVQPVDVGGQNRTPDPPDPNVPASYDGPHGFPHPYPRELGPGA
                     SVVKIGRADPEAPDGSMPDGRIDWAFSTIYRWDQASGTLAPYGKLDRIYHIQGRLDPG
                     YMGTDQAAYFIEICASLSRMDGPVPIKEDGLYPVTYFPAVGPETGTYSMTAAGTNVEG
                     YAILGLPPGSLGGPADLAHGGLSLNQWGVDATLAAGDPVYYPWRFTGEQYAVALRELY
                     AQDNGLLPYTGRPASPYAAFRAVHVEYQPGGGYLIVNGHEDKGEVLVVVPPSVNAATP
                     TNRLRLILPRPEKYGTTNAAAQLWAARDQVDLWPPAPGSAASDRSADERRKRPWIGPA
                     AVEGRPATYRLTQPLAATTGSHY"
BASE COUNT          479 a         1020 c         1144 g          557 t
ORIGIN      
        1 cgggttccat ctccgtcgag gtcggcagca ctccccggtg tgtacgccgg gctccctgga
       61 tcggtggtat tgacggttcc ccagacgagg tccaggtcga cgtcgatctt gatgcgccgc
      121 aggtctcccc atcgaaacac ataggtgccc tcggcgccgc cggtgcggtc cacatccagg
      181 gtcacccagt cgtggatgag cgccggcagc gtgttgccgc cggccacacc cgtatagggg
      241 ttcgcgccgg cggatgggtc ggacaccggc acccaggccg gccggtccgt caccgtggcg
      301 gggttaggct gagccgggag ggcggacggc gcgcggtaga taggcttctc gatcggctgg
      361 aagttcatat accggatgcg cgcgctcccg gggaccgcca tcatacccag agccgccagg
      421 gcgagcacca ggactctgga aggtctcatg ccagttttcg gccgtcccat ggctctcgta
      481 cctccatcac cctcacaggc ccggtccgcc gttcgcgggc cggatctgtc acaacggatc
      541 cacattcagt ctggcgccct agtagtgcga ccccgtggtg gcggccagag gctgagtcag
      601 gcggtatgtc gctggccttc cttcgacggc ggcggggccg atccacgggc gtttgcggcg
      661 ctcgtcggcg gagcgatcgg aggctgccga tccgggggcg ggtggccaca ggtctacctg
      721 atcccgcgcg gcccacagct gggccgccgc attggtcgtg ccatacttct cgggtcgagg
      781 caggatcagc cgcagccggt tggtgggtgt agccgcgttc accgatggtg gcacaacgac
      841 gagcacctca cccttgtcct cgtggccgtt gacgatcaga tagcctcctc cgggctggta
      901 ctcgacatgg accgccctga acgcggcgta gggcgacgcc gggcgccccg tgtagggcag
      961 caggccattg tcctgggcgt acagctcgcg caaggccacc gcgtactgct cgcccgtgaa
     1021 gcgccacggg tagtagaccg ggtctccggc cgccaaggtg gcgtccacac cccactggtt
     1081 gaggctgagc ccaccgtggg ccagatcggc gggaccgccc agcgagccag gcgggagtcc
     1141 gaggatggca taaccctcga cgttggttcc ggcggcggtc atggagtagg ttccggtctc
     1201 gggaccgacg gccgggaagt aggtcaccgg gtagagtccg tcttccttga tgggcacggg
     1261 cccatccatc cggctcaggg aggcgcagat ctcgatgaag taggcggcct ggtcggttcc
     1321 catgtacccg gggtcgagcc gtccctggat atggtagatg cggtcgagct tgccataggg
     1381 cgccagcgta ccgctggcct ggtcccagcg atagatggtg ctgaaggccc agtcgatccg
     1441 cccgtcgggc atcgatccat cgggggcttc gggatcggcg cggccgatct tgactaccga
     1501 ggctcccggg cccagctcgc gcgggtacgg gtgggggaaa ccgtgggggc cgtcatagct
     1561 cgccggaacg ttcgggtcgg gcggatcggg cgtccgattc tgcccaccca catcgacggg
     1621 ctgaacgatg ggcgatgtgc tcaatgcata gttcgccacg gccgccagca cgccgctgaa
     1681 gcccatgggg ttgtccggcc cgccgaacag tcccgggttc atcagcggct ttacagtgat
     1741 gaactcatac ttacccgccg tcttgagctg cccctgggcg ccggtgtcgg gcgttccatc
     1801 gccgtcgaga tccacgggca ggacttcttc gccggccgcg gtggggtcga tgacggcctg
     1861 ggtctgatca taggcgtaga tggcctcgcc gcccgactcg tccaacggag gcaatcggtc
     1921 gccgtcctcg ttggtcagtt gctcgaccgc gatgatgcgg aagttgccgg tgtcggcgac
     1981 gaaggtccac tcgcgtagca ccggctcggg cccggagtag tcgtaccagg cccaccggta
     2041 ggcatcgtgg ggctcgcgaa tctggtggct ggccccggga ggcaggttcg ggtaggccgg
     2101 ccctccatcg ggcggcgcgt agtcgggcgt gtcgcggaag gccgcctcgg ccccctggcc
     2161 ggatcccagg tggttcggat cccagcggtt ctgcagcatc cagtcagcca cgccgtcctt
     2221 gcgcaactcg atgactcgat cgttgccgct gtcggccacc aggtagttgt ttggtttgcc
     2281 ggcgtagtcg agcgccagga ccgattgggg gcgcgagatg cgcaccggca gtgacacgat
     2341 gtcggcggca ttgcccgtgg ggtcgctcac atagagatag cgggccgaag cgacctccca
     2401 gctcggggag ccagccgcgt cgaccgagag cacacggttg aggtcgccga tgacgagccc
     2461 cgaagttccg gcctcgtaga cccggtagaa ccagttccac tggttggcgg cggccgcgcg
     2521 gggcgccacg gagacggtgt agatcctgcc ccggccgacc acggcctgtg tgctggcgat
     2581 cccctcgccc gacgtgagcc cgttcacgac gctgagcagg tcgtacgatg ggttgtgggg
     2641 ctggccgagc gctccgacct gattggtcca gtaggagacc cagccgttgg cctcgccgtc
     2701 atcgatatac cggtagctgg gccacggcgg cgagagggtg gtatcccacg ggaccgacgc
     2761 gccgggccag ggctcgttgg cttcgtcata gtagacgctg tcgcgcgggt ccatggcgaa
     2821 caccagcagc gcctcgctcc gcgtgttggt cagcgggtct ggggcatggg agtaggttac
     2881 gggcacgatg gccttgtcgc tggagatggt gggggagcca atggcggaga ccacataatc
     2941 gggcgatggg tcggcggaca caccgtccat gtcggcatca cgcaccggct cgatgtcgcc
     3001 cgggatgcct gaggtcgtgt cgctggcgcc gagggcatcg atggtgaacg agacgatgcc
     3061 cgtctcgaag tcgatgtcga agggcacgat gccggggttg ttgtcggcat cgttggtgtc
     3121 gaactcgagc ggatcatcga cgtcatcggc atcggcgatc tgcccaccgc tatagctcgg
     3181 ccgcgtgatc gggccgctcg
//
LOCUS       DAOSPL010000401         3199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_30192_length_3199_cov_3.190204, whole genome shotgun sequence.
ACCESSION   DAOSPL010000401 DAOSPL010000000
VERSION     DAOSPL010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30192_length_3199_cov_3.190204"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(303..3014)
                     /locus_tag="PLQ54_08450"
     CDS             complement(303..3014)
                     /locus_tag="PLQ54_08450"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOS93328.1"
                     /translation="MNLAHWGLMALASMFVGLAHAAEPATLFVAPDGSDLSPGTRARP
                     LASLKGARDAIRARRQQGPLPEGGIMVLVDPGLYEISETLTLDIQDSGTAGAPIVYRA
                     ATAEKPTLIGGRVITGWQPDRGAVLRADLRAQGLEGARFRQLFCNGRRVQLARYPDYD
                     AENPVTGGWAYVDGDYVPMYQDLPDEPQDTLRYKPADQREWAHPEEGEVLVFPRYNWW
                     NNIIAIRAVDRDQRTITLAANASYAIRPGDRYFVQGLREELDAPGEWYLDPATSTLYF
                     WPPDGVDPDTMTVYAPVVRDIIRVGRGASHITLRGFLLECCEGTAIALEGTDDCTVAG
                     CLIRNVGDYNGSGVHVEGGRGNGVVGCDIHDVGRDGIALNGGGDPNTLEPGGNYAENN
                     YIHHVGVYYKQGVGVSCSGVGNRVAHNLIHDCPRFGIVWGGNDHVLEYNEIRHTNLET
                     ADCGAIYSWQVDWSKRGTQIRYNYLHDVIGFGWENGKWTSPHMNWGVYLDDGTCGVHV
                     YGNIVVRTILGGVHVHGGRDNVIENNILVDGRDSQVQYSGYVRGGHPVPMMTDTWNQF
                     HGTPAYEKYPGYVELTQSLEDAWQMAGNRFIRNIVCYREPSARLYAHYNLPFDKTESD
                     WNTIWHYGAPLLTGVTKVKESTGPNIAPNPGFEEGEAGGLPAEWQWGVRPKDSDARLD
                     TEVKHSGNQSIRIEGRGTITDASGQSLVVNFNSMPVTLKQGQTYRLTAYIRSDRPGTH
                     CAILPQAYEPGKFWWGKPFGLAAGPEWQQVEGVFRFPAPGDPEWHEDMGSVQIRIDVS
                     GDAASPGIGTIWVDDIELREAEVLNEWEAWQALGLDTHSVMADPLFVDADADDYRLKP
                     ESPAFALGFEPIPVDQIGPYEDDRRASWPIVQAPGAREQMALDWSRR"
BASE COUNT          479 a         1063 c         1082 g          575 t
ORIGIN      
        1 ggcccggcca ccgtctcgct ccagtgcacg aagaaggcgg cctttgccgt gcttaacctc
       61 cgtgccattc gcctcacccc ggccgctcgc taggcgagcg gccgacggcc gggttactga
      121 gcggccaccc acgccctgca ccggcacaca gccggaccat gccgagcctc ctccggccgg
      181 ctcagtgggg gcggcgtttg ggtgaggcgc gcgaatgcac gggccttcgc cggcgctcgc
      241 gggactcgcg gtcgagggtc gggcgatgcg gggcgggaag cgcgtcgggt ccgggcccgc
      301 ccctaccgcc tcgaccagtc caatgccatc tgctccctcg caccgggcgc ctgaacgatg
      361 ggccacgagg cgcgccggtc gtcctcgtag gggccgatct ggtcgaccgg gatcggctcg
      421 aagcccagcg cgaacgccgg cgactccggc ttcaggcggt agtcatcggc gtcggcgtcc
      481 acgaacagcg ggtcggccat caccgagtgg gtgtccaggc cgagcgcctg ccaggcctcc
      541 cactcgttca gcacctctgc ctcccgcaac tcgatgtcgt ccacccagat cgttccgatg
      601 ccgggagacg cggcatcacc cgacacgtcg atgcggatct gcacgctccc catgtcctcg
      661 tgccattcgg ggtcgcccgg cgcaggaaag cggaacacgc cctcgacctg ctgccactcg
      721 gggccggcgg ccaatccgaa gggcttgccc caccagaact tgccgggctc ataggcctga
      781 ggcaggatgg cgcagtgggt gccgggtctg tctgaccgga tgtaggctgt caggcgatat
      841 gtctgccctt gtttgagggt tactggcatg ctgttgaagt tgacgaccag actctggccg
      901 ctcgcgtcgg tgatcgtgcc cctgccctcg atccggatcg attggttgcc cgagtgcttc
      961 acctcagtgt cgaggcgggc atcgctgtct ttgggacgga ctccccactg ccactccgcc
     1021 gggagtcctc cggcctctcc ctcctcgaag cccgggttcg gggcgatgtt gggacccgtg
     1081 gactctttga ccttcgtgac tccggtgagc aacggcgcac catagtgcca gatggtgttc
     1141 cagtccgact cagtcttatc gaacggcagg ttgtagtgtg cgtacagtct ggcggagggt
     1201 tcgcggtagc acacaatgtt gcgaatgaac cggtttcccg ccatttgcca ggcgtcctcg
     1261 aggctctggg tcagctcaac gtagccggga tacttctcgt aggccggagt gccgtggaac
     1321 tggttccagg tgtcggtcat catgggcacc gggtgtccac cacggacata gccgctgtac
     1381 tgcacctgcg agtcccggcc atcgaccagg atgttgttct cgatcacgtt gtcgcgtccg
     1441 ccgtggacgt gcacgcctcc caggatcgtc cgcaccacga tgttgccgta cacgtggacg
     1501 ccgcaggtac cgtcgtcgag gtagacgccc cagttcatgt ggggagaggt ccacttgccg
     1561 ttctcccatc cgaagccgat gacatcgtgc aggtagttgt agcggatctg tgtgccgcgc
     1621 ttgctccagt ccacctgcca cgagtagatg gcgccacagt ctgcggtctc gaggttggtg
     1681 tgacggatct cgttgtactc gagcacgtgg tcgttcccgc cccacacgat gccgaagcgc
     1741 gggcagtcgt ggatgaggtt gtgggcgacg cggttgccga ctcccgagca ggacacgccg
     1801 acgccctgtt tgtagtagac ccccacgtgg tggatgtagt tgttctcggc gtagttcccg
     1861 cccggttcga gggtgttcgg gtctccgcct ccgttcaggg cgatgccgtc ccggcccaca
     1921 tcgtggatgt cgcatcccac gacaccgttg ccgcggccgc cctccacgtg caccccggaa
     1981 ccgttgtagt cgccgacgtt gcggatgagg cacccggcca cggtgcagtc gtcagtcccc
     2041 tcgagagcga tggcggtccc ctcacagcac tcgaggagga agccgcggag ggtgatgtgc
     2101 gaggcgcccc tgcccacccg aatgatgtcg cgcaccacgg gtgcgtacac ggtcatcgta
     2161 tccggatcca cgccgtcggg cggccagaag tagagggtgg acgtagccgg atcgaggtac
     2221 cactcgcccg gcgcgtcgag ttcctcgcga aggccctgga cgaagtatcg atcgccgggt
     2281 cggatggcgt aggatgcgtt cgccgccagc gtgatggtcc gctggtcacg gtctaccgcc
     2341 cggatcgcga tgatgttgtt ccaccagttg tagcgaggga acaccagcac ctcaccctct
     2401 tcggggtgcg cccactcacg ttggtcggcc ggcttgtagc ggagcgtgtc ctggggctca
     2461 tccgggaggt cctggtacat cggcacgtag tcgccgtcga cataggccca gccgccggtg
     2521 accgggttct ccgcatcgta gtccgggtaa cgggcgagtt ggacccgcct gccattgcag
     2581 aagagctggc ggaatcgcgc gccctcaagg ccctgcgccc gaaggtcggc ccggagaacc
     2641 gcgccgcgat ccggctgcca gccggtgatc acccggccgc cgatgagggt cggcttctcc
     2701 gccgtcgccg cgcggtagac gatcggcgcc ccggccgtac ccgagtcctg gatgtcgagc
     2761 gtcagcgtct cggagatctc gtacaacccc gggtcgacga gcaccatgat gccgccttcg
     2821 ggcagcgggc cctgctgccg acgggcgcgg atggcatctc tggccccctt gagggacgca
     2881 agcggccggg cccgggtgcc cgggctgaga tcgctcccgt ccggtgccac gaacagcgtg
     2941 gcgggctcag ccgcatgcgc taggcccacg aacatgctgg cgagtgccat gagtccccag
     3001 tgggcgaggt tcatcggaca acagctcctc tcggtgtctg tcgttcgggc gactggcgcg
     3061 tggtgcctgc aggctgtacg ggatgtaggg aatgagcgcg ggaggaagga tacggggcag
     3121 tcgtgggccg aaatcctctc cagaccaggt cccgggcctc cggtcgacgg gtatgccatg
     3181 ccacggagag aggtcgagg
//
LOCUS       DAOSPL010000402         3195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_30251_length_3195_cov_3.093949, whole genome shotgun sequence.
ACCESSION   DAOSPL010000402 DAOSPL010000000
VERSION     DAOSPL010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30251_length_3195_cov_3.093949"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1438
                     /locus_tag="PLQ54_08455"
     CDS             <1..1438
                     /locus_tag="PLQ54_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93329.1"
                     /translation="LFVSPPMPCPPDSWLSIAVPLYAAPPTPTEPTELADLHLFDTNR
                     SPHDLLIVSLEVSDQLSPAADRVLYPEQWVSRLEERRKAADGTPEAPVLELALARACA
                     WAGDLARAEMLYGGLLAAQALTDAEPAMDLERAELTIEGRQPTDRIRALQAISAKYAG
                     TPEAAWASLALAALSRQTGDVQQAVRMAGSVLNTFNTGPFAGMAMEEAARVRAAQGDF
                     PAAADIWKQSLPLWTNTYDLVAGRERGRRFIADLLAPRGDATPNPAAAVWPAAALFLA
                     PTSTDADLRRIQSEFADSLFAAPAAVKLGRSLKAANDTAGALAQYALTTKQYPFDTEA
                     CAQSRLYTAYALTDDTKRLAELQRAIVDYPDQPRVVGAAYLNIGIRQTAQGKRAEALG
                     TLVQVAQARSGVGEFDLRACWLLAENYEALGETAAALEHWRRVASMAASRSGIAPFDE
                     AGAQAREAIQRLAPSSATAPLPDTMRRP"
     gene            1440..1727
                     /locus_tag="PLQ54_08460"
     CDS             1440..1727
                     /locus_tag="PLQ54_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93330.1"
                     /translation="MTRAHWTRWALAAALLAGGGWFVSTPRPAQADAADDLVRQVAQV
                     ARTLEDTNRELESLRREVEGLRKAVESARLLQVDPTSSSDGPGIPVVIQNP"
     gene            complement(1735..>3195)
                     /locus_tag="PLQ54_08465"
     CDS             complement(1735..>3195)
                     /locus_tag="PLQ54_08465"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOS93331.1"
                     /translation="REHLGSDYAPSWYSPIFPDREDCASIVNSGASRWYNYYLEGLQW
                     LVRYVGIDGLYVDDVSYDRRIMRRVRKILDRNREGCLVDLHSNTGFSRNPAIQYMEFF
                     PFIDRLWFGESFDYDRSPDYWLTEICGIPYGLMGDMLQNGGNPWRGMIYGMTGRMPWS
                     GQPGPMWKAWDDFGIAEARMVGYWDPECPVRTDHDDVLATAYVREGRTMVAVASWATL
                     PVRVRLTVDWEALGIDAAEAHFYAPAIKDFQDEMLFGPDDALVVMPGRGWVLVADELP
                     HEGAPRWTGDVHDTARDARQLAWEQGFDGGDLPAGWTAHVTAKPTAHMSISDGQLHIH
                     CEAHRSAFIERTLSEGATMVEALVSPGTDRGETWGIGLGLAWPDRFLRINIRATEKRV
                     GIDTGQDQWLFPSFVEPRQPVYLRIRLESDLIFLELSADGELWFPVRTLPREAYPGAP
                     TSVRVGKMSGAGRNEDAGLESPEGDCHVEHVWVYAG"
BASE COUNT          549 a         1162 c          999 g          485 t
ORIGIN      
        1 tctattcgtg tcaccgccga tgccgtgtcc ccccgacagc tggctgtcca ttgcggtgcc
       61 tctctacgcg gcgccgccca cacccaccga gcccaccgag ctcgcggatc tccatctctt
      121 cgacacaaac cgctcacccc atgacctgct cattgtgtca ctcgaggtct ccgaccagct
      181 gtcgcccgca gccgaccgcg ttctataccc cgagcagtgg gtgtcacggc tcgaggagcg
      241 tcggaaggcg gccgacggga ctcccgaggc accggtgctc gagctggcgc tggcccgtgc
      301 gtgcgcgtgg gcaggggacc tcgcccgagc cgagatgctc tacggcggcc tgctggcggc
      361 ccaggccctc accgacgcgg aaccggccat ggaccttgaa cgggccgaac tcaccatcga
      421 aggacgccag cccaccgacc gcatccgcgc cctccaggcc atctcggcga agtacgccgg
      481 gacgcccgag gctgcatggg cctcgctggc gctcgccgcc ctcagccgcc agaccggcga
      541 cgtgcaacag gcagtccgaa tggccggatc ggtcctgaac acgttcaaca cgggaccgtt
      601 tgccggcatg gccatggaag aggccgcacg cgttcgagcg gctcagggcg acttccccgc
      661 cgccgcggac atctggaagc agagcctgcc tctatggacc aacacctacg atctggtcgc
      721 cggccgcgaa cggggccgac ggttcatcgc cgacctactg gcaccccgcg gcgacgctac
      781 cccaaacccc gccgcggccg tctggcccgc cgcagccctg ttcctggcac ccacatccac
      841 cgacgccgac ctccgccgca tccagtctga gtttgccgac tcgctcttcg ccgcccctgc
      901 cgccgtgaag ctcggccgat cgctcaaagc ggcgaacgac acggccggcg cgctggccca
      961 gtatgccctg acgaccaagc agtacccctt cgacaccgag gcgtgtgctc aaagccgtct
     1021 gtacaccgcc tatgcgctca ccgatgacac caagcgactc gccgaactcc agcgggccat
     1081 cgtcgactac ccggaccagc cacgcgtcgt cggtgcggcc tacctgaaca tcggcatccg
     1141 ccaaacggcc cagggcaagc gggcggaggc tctcggcact ctcgtacaag tcgcccaggc
     1201 gcggtcgggc gttggcgagt tcgacctccg cgcgtgctgg ctgctcgcgg agaactatga
     1261 ggctctcggc gagacagccg cggccctcga acactggcga cgggtcgcca gcatggccgc
     1321 cagccgcagc ggcatcgcgc ccttcgacga ggcgggcgcc caagctcgcg aggccatcca
     1381 acgcctggcg ccgagctccg cgactgcccc cctgcccgac acgatgagga gaccatagca
     1441 tgacgcgcgc gcactggact cgctgggctc tggcggccgc actgctggcc ggaggcggct
     1501 ggttcgtctc gacaccacgt cccgctcagg ccgacgcggc cgatgacctc gtccgccagg
     1561 tggcccaagt ggcgcggacc ctcgaggaca ccaatcgaga actcgagagc ctccgacgcg
     1621 aggtggaggg cttgcgcaag gcagtcgaga gcgcccggct gctgcaggtg gaccccacca
     1681 gttcgtccga cggccccggc atccccgtcg tcatccagaa cccataaccc tcggctaacc
     1741 agcgtagacc cagacgtgct cgacgtggca atcgccctcg ggcgactcga ggcccgcgtc
     1801 ctcgttccgg cctgcgccac tcatcttgcc cacccgcacc gatgtgggcg cccccgggta
     1861 ggcctcgcgg ggcagcgttc gaacggggaa ccacagctcg ccgtccgccg agagctcgag
     1921 gaagatgagg tcggactcca gccggatccg cagatagacc ggctggcgtg gttcgacgaa
     1981 cgaggggaag agccactggt cctggccggt gtcgatgccg accctcttct cggtcgctcg
     2041 aatgttaata cggagaaacc ggtctggcca ggcgaggccc agaccgatcc cccacgtctc
     2101 gccacggtcg gttccaggac ttaccaatgc ttccaccatc gtggcgccct ctgatagggt
     2161 tctctcaatg aaagcgcttc gatgagcctc gcaatggatg tgcaactggc catctgatat
     2221 gctcatatga gcagtgggct tcgcggtcac atgggccgtc catccggccg gcagatcacc
     2281 gccatcgaag ccttgctccc atgccagctg gcgggcatcc cgcgccgtgt cgtggacgtc
     2341 ccctgtccac cgcggggctc cctcgtgcgg caactcatcg gccaccaaca cccagccgcg
     2401 gcccggcatc accacgagag cgtcgtcggg cccgaagagc atctcgtcct ggaagtcctt
     2461 gatggccggc gcatagaagt gagcctcggc ggcgtcgatg cccagggctt cccagtcgac
     2521 ggtgagccgc acccggaccg gcaacgtggc ccaactggcc acggcgacca tggtcctgcc
     2581 ctcccggaca taggccgtcg ccagcacatc gtcgtggtct gtccgcaccg ggcactcggg
     2641 atcccagtag cccaccatgc gggcctcggc gatgccgaag tcgtcccacg ccttccacat
     2701 cggcccgggt tggcctgacc acggcatccg gccggtcatg ccgtagatca tgccgcgcca
     2761 cggattgcca ccattctgga gcatgtcgcc catgaggccg tagggaatgc cgcagatctc
     2821 ggtgagccag tagtcgggtg agcgatcgta gtcgaaggac tcgccgaacc agagccgatc
     2881 gataaagggg aagaactcca tgtactgtat ggccggattg cgggagaagc cggtgttcga
     2941 gtggaggtcg accaagcagc cctcgcggtt gcggtcgagg atcttgcgga cgcgccgcat
     3001 gatgcgccgg tcgtacgaga cgtcatcgac atacagccca tcgatgccga cataccgcac
     3061 cagccactga aggccctcga ggtagtagtt gtaccagcgc gaggccccgc tgttgacgat
     3121 ggacgcgcag tcttctcggt cggggaagat gggcgagtac catgagggtg cgtagtctga
     3181 cccgaggtgc tcccg
//
LOCUS       DAOSPL010000403         3195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_30253_length_3195_cov_2.635032, whole genome shotgun sequence.
ACCESSION   DAOSPL010000403 DAOSPL010000000
VERSION     DAOSPL010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30253_length_3195_cov_2.635032"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="PLQ54_08470"
     CDS             <1..805
                     /locus_tag="PLQ54_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93332.1"
                     /translation="TVEDCRRIVAAVKKSKRTYMMAENYCYFHYVREWQKLVEAGKLG
                     KIFYAEGEYLHEIINLLVNAKTGQYYWRHERPPIWYCAHCLGPLLTLMDDRIVQACGS
                     HAGFHKHPDQKDHLGFLDAEVGLFRTAKGALIKILRSQVAPRYPHLVYYSLYGQRGYL
                     ENGRFGWGDTDGQVWIQGETPDSPNGHPEAAPMHCALSDPNAPEEALHGGHGTSEYYM
                     IRDFLDAVESGTRPPIDVVRAMDFTVPGIVAHDSAMSDGNWLDVPLFDC"
     gene            833..2188
                     /locus_tag="PLQ54_08475"
     CDS             833..2188
                     /locus_tag="PLQ54_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93333.1"
                     /translation="MRPIRAGFVGFGEVNTPREIIERKCAEAQRALAAEGIELVATAP
                     VSDDPAGADVARAIAELSASDFDALVLCIAGWIPSHAVIAVADVFRHKPMVLWGLTGW
                     TEGGRFITTADQAATTALRKPMVDMGYRFAYIATPKGAPLPVGRVVSYLTAARAAALL
                     RSARIGSMGYRDMRLYGTMHDGVSLRSRIGPEVECFEMLEMTQTIDRLDPAAVSELCE
                     ELPSRWEFVRPPQPATLERTVRLYLAVSGKVRERGYQAVTLIDVDGVKKLLGFAPAGV
                     FMLLHEEAGVCTVPENDILGAVTQLVTRYLTGQVGAYMEFYEFLESGMLMGVPDYVPT
                     EVVDGPVTVLPTAFGDFGEGLLNVSRVKTGRVTLARLTSSGDRYELHLATGTAITPGP
                     WEEAGWVPPAPQLPGLEITLDGDAEAFFQNVASQHYIISYGEHTQVFRDLASILEVGV
                     V"
     gene            complement(2294..>3195)
                     /locus_tag="PLQ54_08480"
     CDS             complement(2294..>3195)
                     /locus_tag="PLQ54_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOS93334.1"
                     /translation="PVGPGAYRYTFSIDGVTVVDPANAATSETNSGVQSLVVVPGSDV
                     MDARDVPHGAVAEVFYQSKSLGRLRRMHVYTPPGYERSEEQYPVLYLLHGAFDCDDAW
                     TTVGRAGFILDNLIAAGKAKPMVVVMPAGHTGPFQFGPSFGADLGKQIGEFASDFLMD
                     IKPYIESHYRIEADQAHRAIAGLSMGGLQTLNIAIPYLADYGYIGVFSSGIFGRQGAA
                     TDTAWEERNKGALDNAEAKQGLRLVWFSTGKDDFLLRTSTATVDMLRKHGFEVTYEES
                     EGGHTWANWRDYLAKFAPLLFGD"
BASE COUNT          517 a         1100 c         1052 g          526 t
ORIGIN      
        1 cacggtcgag gactgccggc gcatcgtggc ggctgtcaag aagagcaaac ggacctacat
       61 gatggccgag aattattgct actttcacta cgtccgcgag tggcagaaac ttgtcgaggc
      121 cggcaagttg ggcaagattt tctatgccga gggcgagtac ctgcacgaaa tcatcaatct
      181 gctggtgaac gcgaagaccg gccagtatta ttggcggcac gagcggccgc cgatctggta
      241 ctgcgctcac tgcctcggcc cgctgctgac gctcatggat gaccgcatcg tgcaggcatg
      301 cgggagccac gccgggttcc acaagcatcc ggatcagaag gatcacctag gcttcctgga
      361 cgccgaggtg ggcctcttcc gcaccgccaa gggcgcgctc atcaagatcc tccgcagcca
      421 ggttgcgccc cgctacccgc acctcgtcta ctactcgctc tacggccagc gcggctacct
      481 cgaaaatggc cggttcggct ggggcgatac ggacggccag gtttggatcc agggcgagac
      541 gccggacagc ccgaacgggc acccggaggc cgctcccatg cactgcgccc tctcggaccc
      601 caatgcgccg gaggaggccc tgcacggcgg ccacgggact tcggagtatt acatgatccg
      661 agacttcctc gatgccgtgg agagcggcac tcggccaccc atcgatgtcg tgcgcgccat
      721 ggacttcacc gtccccggga tcgtggccca cgactcggcc atgagcgacg gcaactggct
      781 ggacgtaccc ctgttcgact gctagctgcc ataccgcccc cgggaggtcg ccatgcgccc
      841 catccgagcc ggattcgtag gcttcggcga agtgaacaca ccgcgggaga tcatcgagcg
      901 caagtgcgcg gaggcccagc gggctctcgc cgccgagggc atcgagctcg tcgccacagc
      961 ccccgtcagc gacgatccgg cgggcgcgga cgtggcccga gccattgcgg agctgtctgc
     1021 cagcgacttc gatgccctcg tgctgtgcat cgcggggtgg atcccctcgc acgccgtgat
     1081 cgctgtggcc gatgtcttcc gccacaagcc catggtgctc tggggcctga cgggctggac
     1141 cgagggcggg cggttcatca ccacggccga tcaagcggcg actaccgcgt tgcggaagcc
     1201 catggtcgac atgggctacc ggttcgccta catcgcgacg cccaaaggcg ctccgctgcc
     1261 ggtcggccgt gtggtcagtt acctcactgc cgctcgggcg gcggcgctgc tccgcagcgc
     1321 gcgcatcggg agcatgggct accgcgacat gcggctctac ggcacgatgc acgatggcgt
     1381 ctcgctccgg tcgcgcatcg ggcccgaggt cgagtgcttc gagatgctcg agatgacaca
     1441 gaccatcgac cgactcgatc cggccgcggt gtcggagctg tgtgaggagc tgccgagccg
     1501 gtgggagttc gtccgaccgc cacagccggc cacgcttgaa cgcacggtgc ggctgtacct
     1561 cgccgtcagc ggcaaggtgc gggagcgggg ctaccaggct gtcacgctca tcgacgtcga
     1621 tggcgtgaag aaactgctgg ggttcgcgcc ggccggcgtg ttcatgctgc tccacgaaga
     1681 ggccggcgtg tgcacggtgc ccgagaacga catcctcggc gccgtgacgc agctcgtgac
     1741 tcgctacctc acgggccagg ttggcgccta catggagttc tacgagttcc tcgagagcgg
     1801 catgctgatg ggcgtccccg actacgtacc caccgaggtc gtggacggcc ccgtcacggt
     1861 tctgcccacg gccttcgggg acttcggcga gggcttgctc aacgtgtcga gagtgaagac
     1921 cggccgagtc accctcgcca ggctgacctc gtccggcgat cgctacgagc tccacctggc
     1981 cacgggcacg gcgatcacgc ccggcccgtg ggaggaggct ggctgggtgc ccccggcgcc
     2041 acagctcccg ggcctcgaga tcacgctcga cggcgacgcc gaggccttct tccagaacgt
     2101 cgccagccag cactacatca tctcgtacgg cgagcacacg caggtcttcc gcgacctggc
     2161 aagcatcctg gaggttgggg tggtatagca ggcgaccgcg ggccgcggcg ctccccatcc
     2221 ccgctgttcc tgaggagccg cgcccgcgcg gcgtcacgaa aggcggcggg cccctcgccc
     2281 cacgcgccgc ccgctagtcg ccaaacagca atggcgcgaa cttggccagg tagtcgcgcc
     2341 agttggccca cgtgtggccg ccctctgact cctcgtaggt gacctcgaac ccgtgcttcc
     2401 tgagcatgtc cacggttgcg gtcgacgtcc tgagcaggaa gtcgtccttg cccgtcgaga
     2461 accagactag ccgcaggccc tgcttcgcct cggcattgtc cagcgccccc ttgttccgct
     2521 cctcccaggc cgtgtcggtg gcggcgccct gcctgccgaa gatgcccgag ctgaacacgc
     2581 cgatgtagcc atagtccgcc aggtagggta tggcgatgtt gagcgtctgc aggccgccca
     2641 tcgagaggcc cgcgatggcg cggtgagcct gatcggcctc gatgcggtag tgcgactcga
     2701 tgtatggctt gatgtccatc aggaagtcgc tcgcgaactc gccgatctgc ttgccgaggt
     2761 ccgcgccgaa tgacggcccg aactggaacg gccccgtgtg ccccgccggc atgaccacga
     2821 ccatcggctt ggccttcccg gccgcgatca ggttgtcgag gatgaagcct gcgcgcccga
     2881 cggtggtcca ggcgtcatcg cagtcgaagg cgccgtggag caggtagagg accgggtact
     2941 gctcctcact ccgctcgtag cccggcggcg tgtagacatg catgcgcctg aggcgtccca
     3001 ggctcttcga ctggtagaac acctccgcta ccgcaccgtg cggtacgtcc cgcgcatcca
     3061 tcacgtccga gcccggcacg accacgaggc tctgcacgcc cgaattggtc tcgctggtcg
     3121 ccgcgttggc cggatccacc accgtgacgc cgtcgatgct gaacgtgtac cggtaggcgc
     3181 cggggccgac gggac
//
LOCUS       DAOSPL010000404         3186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_30352_length_3186_cov_2.988821, whole genome shotgun sequence.
ACCESSION   DAOSPL010000404 DAOSPL010000000
VERSION     DAOSPL010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30352_length_3186_cov_2.988821"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..>3186)
                     /locus_tag="PLQ54_08485"
     CDS             complement(253..>3186)
                     /locus_tag="PLQ54_08485"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOS93335.1"
                     /translation="GATVAVDALVQNAAALKDSEERASVARVLVAVAEGGTQVGIRRY
                     ALRQLSLVGGEESVAPLSRLMKDPQVGEMARWALARIPGRAALQALLVALRTAPEPMQ
                     VGILNALGERGDTEAVEPIAEVMGGAPASVRTAAIAALGHLPSVAAEKVLRPLLTRVS
                     KAERRAARAAYLQLSELLLACGMIAEAEAASLRAYREFPELQDRCAGLAGYAKAAGER
                     AVPALLDAIEAGPADLRGVAMHALAEMPGAGVTPAVIAAMGGASGEAREALLWVVGRR
                     GDGESSTAASAALRSTDERVQLAALRALAALRDPATAEAILAALRRGSERVRAAAEAA
                     LARVPGDEATVAIANAVATAEPAVRVILVRSLGLRPGAVSAAALSSAAGDADEAVRMA
                     CIDAMGRLGDVSTIPALLGRLDAGKEAEWRAAQGALTAMRSADATEALRLAASQGPET
                     IRARVIEALGNRGDAGLYDAFVVLASDPSDAVGAAALDALGKLRDMRAEPVLRRFIQE
                     GTTPKRAAAIRGYLQLGQSVQQKDPGGALAVYLEALDLAPGDAERRLALDRIAEVGEP
                     RALPAVMQWLEKGSPEVAKSAASAAIAIGGKLAQTGQREQAIELLKRAVALSDNREAV
                     RAGAAQLRALGVPLELAKKDGYITHFWVCGPIGSRDELTKSDAFDPARPIDLAVPVRM
                     GDVTRSWRFAPVDDPSGLLDLEKAAARQDNTGAYAYSEITVDQAQDVVLKVGSDDDVF
                     VWVNGKLAGKFVGNRGWTADQDTYEAHLEAGRNTVLLKVLNGGAQWSASCRVLTRDGK
                     PADFAQLQPGEVIGLSGLVSEYWVAAPLPGHDALLKQDALDTTKPVDTSRAVSFGGQS
                     VPWRYTTVGDASGVLNLEAMLGALTDVGAYAYAEVESDRARDVLLKIGSDDEVFIWLN
                     GELVHANETARPVAPDQDTAEAHLEAGVNRVLCKVLQQAGGWGLVVRITDRDGKPIAV
                     KQPRP"
BASE COUNT          521 a         1219 c         1000 g          446 t
ORIGIN      
        1 tgtactgcag tccccaggcg ggcagcgcgc tgcaggccac aagaaccaca agactgccgg
       61 caatcgcggc gcgatgggtc atgttggcat ccgttcctgt ctgtgaggag ccactgcaaa
      121 caagcgaggt gtgcgagcac aatggcccac gccagtgatg ccgccccgcc cgctcgtcca
      181 ctacagaaac cgccggacgg gtgcagggac ccccgcgtcc ggcccagagc ccagacagtg
      241 tggccgcaac acctacggcc gcggctgctt caccgcgatg ggcttgccat cgcggtcggt
      301 gatgcggacc accaggcccc acccgccggc ctgctgaagc accttacaca gaacacgatt
      361 cacgccggcc tcgagatgcg cctcggctgt gtcctggtct ggggccactg gccgggccgt
      421 ctcattggca tgcaccagtt ccccgttcag ccagatgaag acctcatcgt cactaccgat
      481 cttgagcagg acgtcgcgcg ctcgatcact ctcaacctcg gcataggcgt aagcacccac
      541 gtcggtcaac gccccgagca tggcctccag gttgagcacg cccgacgcat cccccactgt
      601 ggtgtagcgc cacggcacag actggccgcc gaaagacacc gcacggctcg tgtcaacggg
      661 cttcgtggta tcgagcgcgt cctgcttgag cagagcatcg tgaccaggca acggcgccgc
      721 aacccagtac tcggacacga gtcccgagag gccgatgact tctccaggct ggagctgcgc
      781 gaaatcggcc ggcttgccgt ctctggtgag cacgcggcaa ctggccgacc attgcgcgcc
      841 gccattcagc accttgagca gcaccgtgtt gcgtccggcc tcgaggtggg cctcgtaggt
      901 gtcctggtcg gccgtccacc cccgattacc cacgaacttg ccggccagct tgccgttgac
      961 ccacacgaac acatcgtcgt cgctccccac cttgaggacc acgtcctgag cctggtccac
     1021 ggtgatctcg gagtacgcgt aagcaccggt gttatcctgc cgagccgcgg ccttctccag
     1081 atcgagcagc ccggacggat catcaaccgg cgcgaaccgc cagctccgcg tcacatcccc
     1141 catcctcacg ggaacggcca agtcgatcgg tctggcgggg tcaaacgcat cgctcttggt
     1201 cagttcgtcc ctcgagccaa tcggtccaca tacccagaag tgcgtgatgt acccatcctt
     1261 cttcgccagc tccagcggca ctccgagggc ccggagctgc gcggcgcccg cacgaaccgc
     1321 ctcgcggttg tccgagagcg cgaccgcccg cttgagcagc tcgatggcct gctcgcgctg
     1381 acccgtctgc gccagcttgc ccccaatggc gatggcggcc gacgccgccg acttcgccac
     1441 ctcgggactg cccttctcga gccactgcat gacggccggc agcgcccgag gctccccgac
     1501 ctcggcaatg cggtcgaggg ccaaccggcg ctcggcatcg cccggggcga ggtccagagc
     1561 ctcaaggtac acggccagcg cgccgccggg gtccttctgc tgcaccgact gacccagctg
     1621 cagataaccg cggattgcgg cggccctctt gggcgtggtg ccctcttgga tgaaacggcg
     1681 cagcacgggc tcggcccgca tatcgcggag cttgcccagc gcgtcgagcg ccgcggcacc
     1741 gactgcatca ctcgggtcac tcgcgagaac cacgaaagca tcgtaaaggc ccgcgtcgcc
     1801 ccggttccca agtgcctcaa tgacccgcgc ccggatggtc tcaggaccct gtgaggcggc
     1861 cagccgcagc gcctcggtag cgtcggcgct ccgcatcgcg gtcagggcac cctgcgcggc
     1921 ccgccactcg gcttccttgc cggcgtcgag ccgacccagc agcgcgggaa tcgtcgacac
     1981 atcccccagc cggcccatgg catcgatgca ggccatgcga acggcctcgt ccgcatcgcc
     2041 cgctgcggat gagagcgccg ccgccgaaac cgcacccggc ctgagtccga ggctgcgaac
     2101 caggatcaca cggaccgccg gctccgccgt ggcgacggcg ttcgcgatgg ccaccgtcgc
     2161 ctcgtctccg gggacgcggg ccagcgcggc ctcggccgcg gcccggacgc gctcgcttcc
     2221 tcgccgcagg gcggccagaa tcgcctcggc cgtcgccgga tcgcgcaacg ccgccaacgc
     2281 cctcagcgcg gcaagctgga cacgctcgtc cgtggaccgg agggcagccg aggccgccgt
     2341 cgacgactcc ccatcgccgc gtcggccaac gacccagagc agagcctccc tggcctcgcc
     2401 gctggcgccg cccatggccg cgatgacggc gggcgtcacg ccagcgcccg gcatctctgc
     2461 cagtgcgtgc atggccacgc cccggaggtc ggctgggccg gcctcgatgg cgtcgagaag
     2521 ggcaggcacc gcacgttcgc ccgcggcctt ggcgtagccc gcaagaccgg cgcaccggtc
     2581 ctggagctcg gggaactcgc ggtaggcacg gagggacgcg gcctcggcct ccgcaatcat
     2641 gccgcacgcc aggagcagct cagagagctg cagataggcc gcacgcgccg ccctccgctc
     2701 ggccttggac acgcgtgtga gcagcggacg cagcaccttc tcggcggcga ccgaaggtag
     2761 gtgccccagc gccgctatgg ccgccgtccg caccgaagcc ggagcgccac ccatcacctc
     2821 ggcgatgggc tccacggcct cggtatcgcc acgctcgccc agggcgttga ggatcccaac
     2881 ctgcatgggc tccggagccg tgcggagcgc gaccaacagc gcctgaagcg ccgccctgcc
     2941 cggtatccgc gccagcgccc atcgggccat ctcgcccacc tgggggtcct tcatcagccg
     3001 gctgagagga gccacggact cctcgccgcc aacgagcgag agctggcgga gcgcgtagcg
     3061 acgaataccc acctgcgttc caccctcggc cacagcgacg agaacccggg ccaccgaagc
     3121 ccgctcctcg ctgtccttca acgcagcggc attttgcacc agggcatcca cagccaccgt
     3181 cgcacc
//
LOCUS       DAOSPL010000405         3181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_30394_length_3181_cov_3.616763, whole genome shotgun sequence.
ACCESSION   DAOSPL010000405 DAOSPL010000000
VERSION     DAOSPL010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30394_length_3181_cov_3.616763"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1975
                     /locus_tag="PLQ54_08490"
     CDS             <1..1975
                     /locus_tag="PLQ54_08490"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS93336.1"
                     /translation="AALPGGSASMADSLTTDERVGHLACWARAFIELAGCDLVCVPSI
                     EVWADQAGVRLHEGAPCQPDSPPTMLSSGERRYWVAGAGAEGFLIARPVDDQRGDGVP
                     QALVAAARAVEMQRHCADRLSLLAAQSRLFQRMGEAASSADGARAAMEELLSGVLDLL
                     RARFGSVMLIDDEIPETMTIFHAKGLPDDIVTSTRVRLGRGVAGTVAQTGEAVLLRRG
                     EAHPLSAGRPEGAYEAAICVPLRVQQRVIGVLNVRGRHDVGDFTPADLELVQALAGLA
                     GPIIEAARLLEAREADIQSALADLQRTNQLLSQAREQFERVLESAPSPAIVVDGNRVV
                     MWVNLRAEKLIGLARHKVQFRPLEEGIAHSSAGAAMVAALDAFETGQPLPRVTLDAPV
                     HRVFQPHVAPLHGDPEQGGGHVILFADVTEIEELSQLKTDLVSVTSHEFRTPLTVMKG
                     AASTLMAHWAVVDEATRSEFLGEIVKQCDRLDRMTRHILDISRIEAGRALDLQRSDVR
                     PDELIGAVVALQTTASDSHRIELRLADNLPIVCVDRDKVEQVLINLVSNAIKYSPDGG
                     VVTIQAEVRGDMLAVSVSDQGIGISEEDQTRLFQRYQRVGGSRSQSIRGTGLGLYLSK
                     GFVESHGGTLTVESTPGAGSTFTFTLPVGSCPS"
     gene            2009..3049
                     /locus_tag="PLQ54_08495"
     CDS             2009..3049
                     /locus_tag="PLQ54_08495"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HOS93337.1"
                     /translation="MIKLFTFPDSVSLANRPTRLYRPRRSLADYSGGREILVKRDDET
                     GCGTSGNKIRKLEFYAAEALQQGADTIITAGHRISNHTRTTALVARELGMDLHLILAG
                     EGECRVDGNLFLEMVAGAEIHFVPPQDRSELNIRLAELADDLRAIGRRPYVVPAGGSS
                     PLGVLGYARCAQEIKQQVDEAGLRIDAITVALGSGSTTAGLILGAEMFGLGCPVYGVH
                     VTSKPELYGPLLRNLIEETRDRFHIATEVPDDRIRVITGYVGNGYGQSSTEDLAFIQD
                     IARRTGLLLDPTYTGKAFRGTLHEMRKGVLQNCERVLFIHTGGLFALYNWRSQFQFAE
                     PSELAPRAQASV"
BASE COUNT          515 a         1057 c         1076 g          533 t
ORIGIN      
        1 cgcggccctg ccgggcggta gcgcgtccat ggccgattcc ctgacaacag acgaacgcgt
       61 gggccatctg gcttgctggg cgagggcttt catcgaactg gccggatgcg atttggtgtg
      121 tgtgccctcg atcgaggtct gggcggatca ggcgggagtc cggctacacg agggggcccc
      181 ctgccagccc gacagcccac ccacgatgct cagctccggt gagcgccggt actgggtcgc
      241 gggcgcgggc gccgaaggct tcctcatcgc ccggcccgtc gatgaccaac ggggcgacgg
      301 cgtcccgcaa gccctggtgg ccgccgcccg agccgtggag atgcagcgcc actgcgccga
      361 tcgcctgtct cttctggccg cacagtcccg gctcttccag cgtatgggcg aagccgcctc
      421 ctccgccgac ggagccaggg ctgccatgga ggagctgctc tcgggcgttc tcgatctgct
      481 ccgggcccgg ttcggctccg tgatgctcat cgacgatgag attcccgaga cgatgacgat
      541 cttccatgcc aaggggctgc ccgacgacat cgttacgagt acccgggttc gtctcggacg
      601 cggtgtcgcg ggcaccgtcg cgcagacggg cgaggcggtg ctgctgaggc gtggcgaggc
      661 ccatccactc tcggcaggcc ggcccgaggg ggcctatgag gctgccatct gtgtccctct
      721 gcgggtccag cagcgggtca ttggagtcct caacgttcgc ggcagacacg acgtggggga
      781 cttcacgccg gcggatctcg agctggtgca ggccctggcc ggcctggccg gacccatcat
      841 cgaggcggcg cggctgctcg aagctcggga ggccgacatc cagtcggcgt tggcggatct
      901 ccagcggacg aaccagctcc tgtcgcaggc gagagagcag ttcgagcgcg tgctcgagtc
      961 ggcgcccagc ccggccatcg tcgtggatgg aaaccgggtg gtcatgtggg tcaacctccg
     1021 ggccgagaag ctgatcggcc tggcgcgcca caaggtgcag tttcgacccc tcgaagaggg
     1081 gatcgcccat tcgtcagcgg gtgcggccat ggtggcggcg ctggatgcat tcgagacggg
     1141 gcagccgctc ccacgggtca cgctggacgc gccggttcac cgcgtgttcc agccccatgt
     1201 cgcgccgctg catggcgatc cagagcaggg cggcggccac gtcatcctct tcgccgatgt
     1261 gacggagatc gaggagctca gccagctcaa gaccgacctc gtctcggtca cctcgcacga
     1321 gttccgcacg ccgctgaccg tgatgaaggg cgcggcgagc acgctgatgg cacactgggc
     1381 ggtggtggat gaagcgacgc gcagcgagtt cctgggcgag attgtgaagc agtgcgaccg
     1441 cctcgaccgg atgacgcggc acattctgga catctcgcgc atcgaggcgg gacgagccct
     1501 ggacctccag cgctcggacg tgcggcccga cgagctcatc ggagccgtcg tggctctgca
     1561 gacgacggcg tcggactcac accgcatcga gttgcggctg gccgacaacc tgcccatcgt
     1621 gtgcgtcgac cgcgacaagg tggagcaggt actcatcaac ctggtctcga atgccatcaa
     1681 gtactcgccc gacggtggcg tcgtgacgat ccaggcggag gtccgcggcg acatgcttgc
     1741 cgtgagcgtc tccgaccagg gcatcgggat cagtgaggag gaccagacac gcctgttcca
     1801 gcgctaccag cgtgtgggcg gcagccgctc gcagtccata cggggtactg gtttggggct
     1861 atacttgtcc aaggggttcg tcgaatcgca cggcgggacc ctcaccgtcg agagcacgcc
     1921 gggggccggg tcgaccttca ccttcactct acccgttggg agttgcccct cctaagcgcc
     1981 gggctctcag ttccccgcat caatggagat gatcaagctg ttcacgtttc cggattcggt
     2041 aagcttggcc aatcggccca cgcgtctgta ccgcccacgc cgatccctgg ccgactactc
     2101 gggcgggcgc gagatcctgg tcaagcgcga tgatgaaacc ggttgcggca catcgggcaa
     2161 caagatccgc aagctggagt tctacgcggc cgaagcgctc caacagggcg ccgataccat
     2221 catcaccgca ggtcaccgca tcagcaacca cacccgcacc acggctctgg tcgcccgcga
     2281 actcgggatg gacctgcacc tgatcctcgc cggtgagggc gagtgtcggg tcgatggcaa
     2341 cctgtttctc gagatggtgg ctggcgctga gatccacttc gtcccgcctc aggaccgctc
     2401 agagctcaac atccgcctgg ccgagctggc ggatgacctc cgggcaatcg ggcggagacc
     2461 ctacgtggtg cccgccggcg gcagttcccc gctcggcgtg ttgggctatg ctcgctgtgc
     2521 gcaggagatc aaacagcagg ttgacgaggc gggacttcgc atcgacgcca tcactgtcgc
     2581 cctgggcagc ggaagcacga ctgcgggcct tatcctcggc gccgagatgt tcggcctggg
     2641 gtgcccggtc tacggcgtgc acgtcacctc gaagcccgag ctctacggcc cgttactgcg
     2701 caacctgatc gaagagacgc gcgaccgttt ccacattgcc accgaggtgc ccgacgaccg
     2761 gatccgggtg atcacagggt atgtgggcaa cggttacggc cagtcaagca ccgaggatct
     2821 cgcgttcatt caggatatcg cccgtcgaac gggtctgctg ctcgacccga cgtacaccgg
     2881 taaggcattc cgcgggacgc tccacgagat gcgcaagggc gtcctgcaga actgtgagcg
     2941 agtgctcttc atccataccg gcggcctctt cgcgctctac aactggaggt cccagttcca
     3001 gttcgcggaa ccctccgagc tcgccccgcg ggcccaggca tcggtgtagc ccgacgctgc
     3061 ggggaccagg ctgccgttcc ggaggaggcc gctgcgtgcc cgtgctggtc tacaatgacg
     3121 acggctggtc cacgtacatg cggtacccgc ggtccgtggg cacccgcccc gcggaggtgt
     3181 g
//
LOCUS       DAOSPL010000406         3177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_30454_length_3177_cov_2.795003, whole genome shotgun sequence.
ACCESSION   DAOSPL010000406 DAOSPL010000000
VERSION     DAOSPL010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30454_length_3177_cov_2.795003"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3091
                     /locus_tag="PLQ54_08500"
     CDS             <1..3091
                     /locus_tag="PLQ54_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOS93338.1"
                     /translation="AELGGTGAGRADVVAVVCTGHGWGVIPAAALREAGDEPAEVRLQ
                     PGGTVRARCRGADGSVRAGTSVALRSVHGWEVGSQSRTGEAGADGLAVFADLPPGVYE
                     LDVPSGRTATQTVVLAPGDDVTTDVWVASDGGSGTGAPRMASGHTPEAGDLSGVVVAA
                     DDGPPGPVSVTLDVFPLDGMAAGSSPRHQEIAGGTGPFRFDSVLEGYHVLGVQSGERT
                     VALRPLYVPPPDERKPIRITVSRQFGSVAGRVVDSASGQGVAGVTVLAIERHYVYGVH
                     QQYAHAVHYDTRTREVRPGSLGSEAAPLNLAAILPRASSDADGRYMIGGLLPGAYILV
                     AIDEQQRRDELHPMAVRANATTEAPDLRLPSPEGIPLAVRLVTESGEAVSGESIEVAT
                     MSGPPGGTLRPDGPTDGSGRATLLARGPGWYGFSLRLANGAQASCWGAVVPTAGTAPE
                     VTLTVSDPGPGEATVQLLDVDGSPPKGRVWVVPLVYRPAHGAVVAETHLGRFADERGR
                     CELRDLRPGIYAFLARTPSPAGTDSLGVLMGVSDTVPVAPGAPAGVTVVLEPCSRIAG
                     RVTAPDGSVLTRLFVSADQSYGMLPGHTSRHSVVTDEAGEFVLDGLPPARWSVQCQPW
                     DTAEGLVFPSTEVVPAPGEMTWLDVAGRDASDVETTGATEPSQYLTVTGKVSWPDGSP
                     ANGVVVGALDSDWGVPKHSITAEDGRFVLAGLPRWNAGLFAWSPEGVSARVSLDRRGD
                     ADDAPPLELVLEPGRSIRGRVHMEVGDPAAGGLEAMAVRADRHWATQELWPGEGSCVR
                     VPVDPDGSFSIHGLAPGRYQVRAVWGNLVRSAPVTVELAAGDAEGLSLAVPPLVPELR
                     VALQDADGRPLAQGLSVNVAVHDIHIAGNLHVVAGNLVLSHVPPGRYSFHGQSADNRS
                     IQLGSADVEPGATTVTVQQPSTNRVEGRVVVPEGFALHGGLRVAMCREGDWLGYSTRY
                     ASIGPDGSYRLAADGGGEFEVCLVAGRATRLAVRKVSLRGEQMAEVPDLVYEGPVAR"
BASE COUNT          435 a         1023 c         1203 g          516 t
ORIGIN      
        1 ggcggagctg ggcggtacgg gtgctggccg cgccgatgta gtggcagtgg tgtgcacggg
       61 ccacgggtgg ggagtgatcc ccgctgccgc acttcgcgaa gcgggggacg aaccagcaga
      121 ggtgcgtctg caaccagggg gcacggtgcg tgcccgttgc cgcggcgccg acggcagcgt
      181 tcgggccggc acctcggtgg ccctacgcag cgtgcatggc tgggaggtcg ggagccagtc
      241 gcggaccggg gaggccggcg ccgacggtct cgcggtcttc gctgatctgc ctcccggagt
      301 ctatgagttg gacgtcccaa gtggccggac tgccacccag accgtggtcc tcgcgccggg
      361 tgatgacgtc acgaccgatg tgtgggtggc ctccgatggc ggctccggaa ctggggctcc
      421 gcggatggcc tcaggccaca cacctgaagc cggggatttg tcgggtgtgg tggtggcggc
      481 cgatgacggc ccgccgggcc ccgtgagcgt caccctggac gtgttccctc tcgacggaat
      541 ggcggcgggg tcgagcccgc gccaccagga gatcgcgggc ggcacgggac cgttccgctt
      601 cgattcggtc ctcgagggct accatgttct tggtgtgcag tccggagaga ggacggtcgc
      661 gcttcggcca ctatacgtgc cgccgcccga tgagcggaag ccgatcagga tcacggtctc
      721 ccggcagttc ggcagcgtcg cgggacgagt ggtggattcg gcgtcgggtc agggcgtggc
      781 gggggtgacc gtgctcgcca tcgagcgaca ctatgtgtac ggggttcacc agcagtatgc
      841 gcacgccgtc cattacgata cccgcactcg cgaggtgcgc cccggcagtc tgggctccga
      901 agcggcgccc ctgaacctgg ccgccattct ccccagggca tcgagtgacg ccgacgggcg
      961 gtacatgatt gggggtctcc tgccgggtgc gtacattctg gtcgccatcg atgagcagca
     1021 gcgccgtgac gaactccatc cgatggccgt ccgggccaac gcgacgaccg aggcgcccga
     1081 cctgcgcctt ccctcacccg aaggcatccc actggccgtg cggctggtta ccgagtcggg
     1141 ggaggccgtc tctggcgaga gcatcgaagt ggcaacgatg tcgggccctc cggggggcac
     1201 cctgcgccca gatggcccga ccgacggctc cggtcgagcc accttgctgg cgcgaggccc
     1261 cggctggtat gggttcagcc tccggctggc caacggcgcc caggcgagtt gctggggtgc
     1321 ggtggttcca acggctggca cggcgccgga ggtgacgctc accgtgtcag accccgggcc
     1381 cggcgaggcc acggtgcagc tgctcgacgt agacggcagc ccgcccaagg gccgggtgtg
     1441 ggtcgttccg cttgtctacc ggcccgccca cggggcggtg gttgctgaga cccacctcgg
     1501 ccgtttcgca gacgaacggg gtcgctgcga gctgagagac ctgcgtccgg ggatctacgc
     1561 attcctcgcg cgcaccccat cacccgcggg gaccgacagc ctgggtgtac tgatgggggt
     1621 ctccgacacc gtaccggtgg cgccaggtgc tccagcaggc gtgacggtcg tgctcgagcc
     1681 ctgcagccgg atcgcggggc gcgtcacggc accggacggc tcagttctca cgcggctctt
     1741 cgtctccgcc gaccagagct acggcatgct gccgggccac acgtcgcggc actcggtggt
     1801 caccgacgag gcgggcgagt tcgtgctcga cggtctgccg ccggctcgtt ggagcgtgca
     1861 gtgtcagccc tgggacacgg ccgagggtct cgttttcccc tccaccgagg tcgtgccggc
     1921 accgggggag atgacatggc tcgatgtggc tgggcgtgac gcctccgacg ttgagacgac
     1981 cggagccacg gagccatcgc agtacctcac ggtaaccggg aaggtgagct ggcctgatgg
     2041 gagcccggcg aacggcgtgg tcgtgggcgc cctcgacagc gactggggtg tcccgaagca
     2101 ctcgatcacg gcggaggatg gccgcttcgt cctggcgggc ctcccacgat ggaacgctgg
     2161 cctgttcgcg tggagccccg agggagtctc ggcccgggta tcgctggacc ggcggggtga
     2221 tgcggacgac gctccacccc tggagctggt gctcgagccc ggccggtcga ttcggggccg
     2281 tgtgcacatg gaggtcgggg acccggcagc cggggggctc gaggcgatgg cagtccgtgc
     2341 cgatcggcat tgggccactc aggagctctg gccgggtgaa gggtcgtgcg tacgggtccc
     2401 ggttgaccct gatggttcat tctcaatcca tggacttgcg ccaggccggt accaggtcag
     2461 ggccgtctgg ggcaacctgg tgcgctcagc cccggtcacc gtcgagctcg ctgcgggcga
     2521 cgccgagggc ctgagcttgg ccgtgccgcc gctcgtgccg gaacttcggg tcgcgctcca
     2581 ggatgcggac ggcagaccgc tggcccaagg cctttccgtc aacgtggccg tgcatgacat
     2641 tcacatcgcc gggaatcttc acgtggtggc cggcaacctg gttctcagcc acgttcctcc
     2701 tggacggtac agcttccacg ggcagtccgc ggacaaccgg tcgatccagc tgggctcggc
     2761 cgatgtggaa cccggggcta ccacggtgac ggtccagcag ccctccacga accgggtcga
     2821 gggtagggtg gtggtgccag agggcttcgc cctgcacggc gggcttcgcg tcgccatgtg
     2881 ccgcgagggg gactggctgg ggtactcgac gcgctatgcg agcatcgggc ccgatgggag
     2941 ctaccggctc gcggccgacg ggggcggcga gttcgaggtg tgcttggtcg cgggccgcgc
     3001 cacacgcctc gcagtgcgca aggtgagcct gcggggggag cagatggcag aggtgcccga
     3061 tctggtctac gagggcccgg tggctcgcta ggagccccgg tgactgggcg tgcgcctcga
     3121 tcggaggtag tcggatggta cggtgcatcg gtggtctgct ggcttggtcg atgatcg
//
LOCUS       DAOSPL010000407         3168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_30578_length_3168_cov_3.814006, whole genome shotgun sequence.
ACCESSION   DAOSPL010000407 DAOSPL010000000
VERSION     DAOSPL010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30578_length_3168_cov_3.814006"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="PLQ54_08505"
     CDS             complement(<1..1488)
                     /locus_tag="PLQ54_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93339.1"
                     /translation="MGGVLLAAIPAVLVAGALTVLLSAPVVRALSERTGDRADPQTAS
                     AVTATLVCVLALVAALGAWHSPALVVFGSSVSDRWGLCMDGLAGPVLAAMLALFAVAC
                     ATGLLRGTSHAVPFPLLLALAAAGTLLVLAQNLLMLALAIEAAVALGALLVARGGPQQ
                     ASGPSTPLRYLAVSQVGALLLVIVLVRLSSLAHAPVAGLPELPLYELGDLRARVSMGG
                     PHVLGTLAALWVLGFVAWAGAANPWVLPDAARRYSSPVLVSIAGGLFAAQGMVFIRVA
                     LSVFPYECWESASAVTRSLAVVLAVAGAAGALWARRPGSAFAWALLSHVGVLALAVQL
                     GDRPGAAGVAFALIGLAAARAGLAASASEGPWCFAAPSALTGGAAAALAGLTLVLGAA
                     ALPLASTGHGWRPGAVALAMLAVALAAYAVRWGLYCWHEWSRRAHGRIGLVAVCALTA
                     LAAVPLPGRDAVLRQIEATLSLERRIIAPPIHGGGGAGGPSAPGPG"
     gene            1794..2156
                     /locus_tag="PLQ54_08510"
     CDS             1794..2156
                     /locus_tag="PLQ54_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS93340.1"
                     /translation="MTSEERDEIFKRAEKLRINGDYEAARPLYEELLGADGNDHGAVL
                     GLAYCQLNIGEFEEAIETFQRAKEVDPTHVKGRLELAKSMAMLAMYDEAKAEFLGVLE
                     LDPENEDALEQLEYFPDD"
     gene            2266..>3168
                     /locus_tag="PLQ54_08515"
     CDS             2266..>3168
                     /locus_tag="PLQ54_08515"
                     /inference="COORDINATES: protein motif:HMM:NF036965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOS93341.1"
                     /translation="MHSVNVKFEPDNVRGQVAAGSTVAEAASHLAVPIAQPCGALGQC
                     GKCQCVVRAARVEETGDQRAVLGDSLYDRGVRLACQTRLLSDAEIEILPVARVGEQRI
                     LVDASTDLAAVEPDVRLSKTYLELDPPTLTDQRADLDRIVDALAAEGVRVEVTEAPRM
                     VRGLAHLCRASQYRVTAVAADGGLVAVEPGDTRAHAYAVAVDIGTTTVVAYLADLRSG
                     HRRAVASAVNPQVAYGDDVVSRIRYLQDHEDGIVTLQGAVVSTVDGLIGELVTEAGID
                     DAWIYRVVVVGNATMTHLLWGADPS"
BASE COUNT          590 a         1045 c         1126 g          407 t
ORIGIN      
        1 tccggggccg ggcgcgctgg gccccccggc gcctccacca ccatggatgg gtggggctat
       61 gatccgcctt tcgaggctaa gggtcgcctc aatctgacgc aacacagcgt ctcgccccgg
      121 gaggggcacg gcggccaggg cggtgagcgc gcacacggcg accaggccga tccggccgtg
      181 cgccctgcgg gaccactcat gccagcagta gagcccccag cgaaccgcgt acgctgccag
      241 tgcgaccgcc agcatggcga gagcgacggc gcccggccgc caaccatggc cggtggaggc
      301 caggggtaga gccgccgctc cgagcaccag ggtcaggccg gccaacgcgg cggctgctcc
      361 cccagtcagg gcggaagggg ccgcaaagca ccaggggccc tcggaggcgg aggcggcgag
      421 acccgctcgg gccgccgcaa ggccgatcaa cgcgaaagcc actccggccg cgccagggcg
      481 gtcgccgagt tgaacggcca gggcgagcac acccacgtgt gacaagagcg cccaggcgaa
      541 cgcgcttcca ggccgccggg cccagagagc gcccgccgca cctgccaccg caagcaccac
      601 ggccaggctg cgcgtcacag ccgaggcaga ctcccagcac tcatagggaa acacgctcag
      661 agccacgcgg atgaacacca tgccctgcgc cgcgaacagc cccccggcga tcgagaccag
      721 gaccggcgag ctgtaacgcc gggcggcgtc ggggaggacc cagggattcg cggcgccggc
      781 ccatgccacg aagccgagga cccagagcgc cgcgagggta cccaggacgt gggggccgcc
      841 catcgacacg cgggcgcgca agtcccccag ttcgtagagc ggaagctcgg gcaggcccgc
      901 aaccggcgcg tgtgccaggg acgacagtcg aaccaagacg atgaccagaa ggagagcgcc
      961 cacctgggac acggccagat aacgcagagg agtcgatgga ccgctcgcct gctggggtcc
     1021 ccctcgagca accaggaggg ccccaagggc gaccgccgcc tcgatggcca gggcgagcat
     1081 gagaagattc tgagccagaa cgagaagcgt accggcggcg gccagggcaa ggaggagcgg
     1141 gaaagggact gcatggctcg tgccacgcag aagacccgtg gcacaggcga cggcgaagag
     1201 agcgagcatg gccgcaagaa cggggcccgc aagaccatcc atgcagaggc cccagcgatc
     1261 gctgactgat gagccaaaca ctacaagcgc aggggaatgc cacgctccca gtgcggcgac
     1321 gagagccaga acacacacaa gggtcgcggt cactgcggag gccgtctgtg ggtcggcccg
     1381 gtcaccggtg cgctcggaaa gcgctcgcac caccggggca ctgagtagga ccgtcagcgc
     1441 tccagccacc aacacggcgg ggattgccgc caacaacacg ccacccatgg gtcgaccgcc
     1501 cttaccgacg cggagaggcg gatgtccgcc ctcgctgcca ttatagcagt cgttgttcgt
     1561 gcgacggcgc ggcgggacga gcgtcgcgaa agcgcaagaa agatcagcga gcgcagaggc
     1621 cttttccggc acgaggcaga acatggcggt gttgaacagg gggcgatgca ggggccggga
     1681 tattccaggc tcttaccggc cgcgcagcgc tacgctgatg caactcggcg aggttggggc
     1741 ggcaagcctt gacttcggcc cgcgtttggg gccagggcga agctgggaga gagatgacgt
     1801 ccgaagagcg cgacgagatc ttcaagcgcg ccgagaagct gcgtatcaac ggcgactatg
     1861 aggcggcgcg ccctctgtac gaagagctgc tgggcgcgga cggcaatgac catggtgccg
     1921 tgctcggact ggcctactgc cagctgaaca tcggcgagtt cgaggaggcc atcgagacgt
     1981 tccagcgcgc caaagaggtg gaccccaccc atgtcaaagg ccggctggag ctcgccaagt
     2041 ccatggcgat gctggccatg tacgatgagg ccaaggccga gttcctgggt gttctggagc
     2101 tggatcctga gaacgaggat gccctcgagc agcttgagta cttccccgac gactaggctg
     2161 taccgcagca tgacatgact gctggcgtcg accccaaagg cccatcgggg tcgtcttgtt
     2221 gtacccggag cctccccatg cagttccggt agtggtgggt gaaccgtgca cagcgtcaac
     2281 gtcaagttcg agccggacaa cgtacgtggc caagtggcag cggggtccac ggtggccgag
     2341 gccgcgtccc acctggcggt gccgatcgcg cagccttgtg gcgccctggg gcagtgcggc
     2401 aagtgccagt gtgtcgtccg cgccgcgcgc gtcgaggaga ccggtgacca gcgcgccgtt
     2461 ctcggcgatt cgctgtacga ccggggagtg cggcttgcat gtcagacccg gctcctctcc
     2521 gacgcggaga tcgagattct gcccgttgcc cgagttggcg agcagcggat cctggtggac
     2581 gcctcgacgg acctggccgc ggttgagccc gatgtgcggc tctcgaagac gtacctggag
     2641 cttgatcctc ctacgctgac cgaccagaga gccgacctcg accggatcgt cgacgccctg
     2701 gcggccgagg gagtccgggt cgaggtcacc gaggctccgc gcatggttcg tggtctcgcc
     2761 catctctgcc gtgcgagcca gtaccgagtc acggcggtag cggccgacgg cggcctggtc
     2821 gccgtcgagc cgggcgacac gcgcgcccac gcctacgcgg tggccgtgga catcggcaca
     2881 accacggtgg tggcgtacct ggccgacctg cgctcgggcc atcgccgggc cgtcgcatcg
     2941 gcggtaaacc cccaggttgc atacggcgac gacgtggtat cgcgtatccg ctatctgcag
     3001 gatcacgaag acggcatcgt gacgcttcag ggcgctgttg tgtcgaccgt cgacgggctg
     3061 atcggcgagc tggtgactga ggccggcatt gacgacgcct ggatctaccg ggtcgtggtg
     3121 gtcggcaatg ccaccatgac gcacctgctg tggggcgccg atccgtcg
//
LOCUS       DAOSPL010000408         3167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_30594_length_3167_cov_3.390424, whole genome shotgun sequence.
ACCESSION   DAOSPL010000408 DAOSPL010000000
VERSION     DAOSPL010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30594_length_3167_cov_3.390424"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2069)
                     /locus_tag="PLQ54_08520"
     CDS             complement(<1..2069)
                     /locus_tag="PLQ54_08520"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOS93342.1"
                     /translation="MSSSELGNDWSDVLLTEGAEPTITYRSGLVVYEESLTQGRLVGR
                     GWNASGYVNFYDGRLNPAEHATPQAFWLEVDGQLLAWDWEWQGLEEVATEAHTRHMVV
                     SLRHLVRPVTAKVHTRLDGTPILTRWLEVTNTGTQPAALSSVASWSGVLQKTSRWRLL
                     MGGGDAPLYSIGYFANSHWGNEGDFQWFDLPQAGYRVDGRYRRDRHRHPMVVVRNNAT
                     GEHFIWQLAWTGGYSFEFDLDADAGTMDQAARLAFRAGPDAPAPLRIIAPGETIATPE
                     VHLGAAFGDLDTVIQAMHDHLRKSVFMPQPRGRGGWVESGIGPEIEITSDQVIHAIDA
                     AAEFGAEVFFIDASWYAKPGGNWWSTVGDWREDRERFPEGLASFRKRVHEKGMLWGLW
                     MDAERIGNESGVCKEHPDWLAAGYDGQRNLGGLLDLTNPDVARWMEGTIARVIEEYGL
                     EFFRLDHNTHPGPGLQTRRDGFVENGYFRYYEALYGIYDRLRRRFPDVIFENCAGGGG
                     RTDIGLVRHFSHTWVTDWQIAPRSFAITNGMTMALPPEYVDRLIGGQSGHTTAEFEFQ
                     TRLLLFVRPTIGFPKPMGSNWNPALLERLKHAVGLYKDFVRPFMSTGRIYHHTPCVPN
                     PEPRGWGVLELASHDRTRAICGLFQLAAPTEPEYVLRLRGLDRSRRYRVMFDNSGQTA
                     EIEGMVL"
     gene            complement(2187..>3167)
                     /locus_tag="PLQ54_08525"
     CDS             complement(2187..>3167)
                     /locus_tag="PLQ54_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93343.1"
                     /translation="YVGVDREHPPEESGYSEFDIREFEKDRGEPVGGTAGDAQSRYDW
                     LKANAWDEWIEWRCQRIHDHWLRLAAAVRAVDPAKQLIVFTKIPANDPGEKRDWESGP
                     VDLLTLHKYHGYDPALYTADRGLTLSRVMGVNADRYWLQRWNKEFFFQPPIDGFFRTA
                     SPSGVELYYIYWELPDHPLGFRVGPASPRGRAFYEPMTHALRYQNPGSFCFYNWFRAT
                     MGHETDLREFCRAYRGLPMVDPQPFPGRVEPAAAAADERLCVRMFRDRISIVNDGPEA
                     REITLVLPAGYAMRGLRDLALNTRCDIERRGGRREVRLQIRPWDIRTLAP"
BASE COUNT          512 a         1079 c         1034 g          542 t
ORIGIN      
        1 atcagaacca tcccctcgat ctcggcggtc tggccgctgt tgtcgaacat cacccggtac
       61 cggcgtgatc gatccaggcc ccgcagtctg agcacgtact cgggctccgt cggtgcggcg
      121 agctggaaca ggccacagat ggcccgggtg cggtcatgtg aggcgagttc cagaacaccc
      181 caccctcgcg gctccggatt gggcacgcac ggggtgtggt ggtagatgcg ccccgtgctc
      241 atgaaaggcc gcacgaagtc cttgtagagg ccaacggcgt gcttgaggcg ttccagcagt
      301 gctgggttcc agttggagcc catgggcttg gggaagccga tcgtcggccg gacgaacagc
      361 agcaggcggg tctggaactc aaactcggcc gtggtgtggc cgctctggcc gccgatgagt
      421 cgatccacgt attccggcgg cagggccatg gtcatgccat tcgtgatcgc gaacgagcgg
      481 ggcgcgatct gccagtcagt cacccatgtg tggctgaagt gtcgcaccag gccgatgtcg
      541 gtccggccgc cgccgccggc gcagttctcg aaaatcacgt cggggaaccg cctgcgcagc
      601 cggtcataga tgccgtacag ggcttcgtag taccggaagt acccgttctc gacgaacccg
      661 tcgcggcggg tctgcagacc ggggcccgga tgggtgttgt ggtcgaggcg gaagaactcg
      721 agaccatact cctcgatgac ccgcgcgatg gtcccctcca tccaccgtgc cacgtcgggg
      781 ttggtcaggt cgaggagtcc acccaggttc cgctgtccgt cgtacccggc cgcgagccag
      841 tccgggtgtt ccttgcagac gccgctctcg ttgccgatgc gctcggcgtc catccatagg
      901 ccccagagca tgcccttctc gtgcacccgc ttgcggaatg acgccagacc ctccgggaaa
      961 cgctctcggt cctcccgcca gtcgcccacc gtgctccacc aattgccgcc aggcttggcg
     1021 taccaactcg cgtcgatgaa gaagacctcg gccccgaact cggcagccgc gtcgatggcg
     1081 tgaatgacct gatcagacgt gatctcgatc tcgggcccga tgcccgactc cacccatccg
     1141 ccgcggcctc ttggttgagg catgaatacg ctctttcgga gatgatcgtg catggcctgg
     1201 atgacggtgt ccaggtcgcc gaaggccgct cccaggtgca cctccggcgt ggcgatggtc
     1261 tccccgggag cgatgattcg tagcggcgcc ggtgcgtcgg gcccggcacg aaatgccaat
     1321 cgtgccgcct ggtccatcgt gcccgcatcc gcgtcgagat cgaattcgaa gctgtaccca
     1381 cccgtccagg ccaactgcca gatgaagtgt tcgcccgtgg cgttgttccg gaccactacc
     1441 atcgggtgcc gatgacggtc tcggcgatag cggccgtcca cccggtaacc ggcctggggg
     1501 aggtcgaacc actggaagtc accctcgttg ccccagtggc tgttggcgaa gtagccgatc
     1561 gagtaaagag gggcgtcgcc gccgcccatg agcaagcgcc agcggctcgt cttctgcagc
     1621 actccgctcc acgaggccac tgacgagagt gccgcgggct gggtccccgt gttcgtgacc
     1681 tcgagccaac gcgtcaggat gggcgtgccg tcgagcctcg tgtggacctt cgccgtgacc
     1741 ggtcgcacca gatgacgcag gctgacgacc atgtggcgtg tgtgggcctc ggtagccacc
     1801 tcctcgaggc cctgccactc ccagtcccac gcgagcagtt gcccgtcgac ctccagccag
     1861 aatgcctggg gcgtcgcatg ctcggcgggg ttgagtcggc cgtcgtagaa gttcacgtac
     1921 cctgaggcat tccacccgcg gcccacgaga cgtccctgag tcagcgactc ctcatatacg
     1981 accaagccgc tgcggtaggt gatcgtcggc tcggccccct cagtcagcag gacatcggac
     2041 cagtcgttac caagctcgct gctgctcatg tcgggctccc tctactcggc catcggccaa
     2101 cgaccacgca ctgcggcaca cgccgcccag ggcatcggtt tcggcagcaa tccgagccgt
     2161 cctgccaggg cagacggcag cggagctcac ggcgccagag tgcggatgtc ccagggcctg
     2221 atctgcagcc gcacctcgcg ccgtccgccc cggcgctcga tgtcgcatcg cgtgttgagg
     2281 gcgagatcgc gcaggccgcg catcgcgtag cccgcgggca acacgagggt gatctcgcgt
     2341 gcctccgggc cgtcgttcac gatgctgatg cggtcgcgga acatccgcac acagaggcgc
     2401 tcatcggcgg ctgcggcagc cggctcgacc cgaccgggga acggctgcgg gtcgaccatt
     2461 ggcagcccgc ggtaggcgcg gcagaactcc cgcaggtccg tctcgtggcc catggtggcg
     2521 cggaaccagt tgtagaagca gaagctgccg gggttctgat accgcagggc gtgcgtcatc
     2581 ggctcataga acgcgcggcc acgcggtgag gccggcccga cgcgaaagcc caacgggtga
     2641 tcgggcagct cccagtagat gtagtagagc tcgacgccgc tgggcgaggc cgtccggaag
     2701 aacccgtcga tcggtggctg aaagaagaac tccttgttcc agcgctgcag ccagtaccgg
     2761 tcggcgttga cgcccatcac ccgtgagagg gtcaggccgc gatctgcggt gtacagcgcc
     2821 gggtcgtagc catggtactt gtggagcgtc agtaggtcga cgggcccact ctcccagtcg
     2881 cgcttctcgc ccgggtcgtt cgcggggatc ttcgtgaaga cgatgagctg cttcgccgga
     2941 tcgaccgcgc gcacggccgc ggccaggcgt agccagtggt catggatccg ctggcaccgc
     3001 cactcgatcc actcatccca cgcattcgcc ttcagccagt cgtaccggct ctgggcgtcg
     3061 cccgcagtgc cgcccacggg ctccccacgg tccttctcga actcgcggat gtcgaactcg
     3121 ctgtaccccg actcctccgg cgggtgctcg cggtccacgc ccacata
//
LOCUS       DAOSPL010000409         3167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_30596_length_3167_cov_2.925129, whole genome shotgun sequence.
ACCESSION   DAOSPL010000409 DAOSPL010000000
VERSION     DAOSPL010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30596_length_3167_cov_2.925129"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..1895)
                     /locus_tag="PLQ54_08530"
     CDS             complement(168..1895)
                     /locus_tag="PLQ54_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS93344.1"
                     /translation="MLDLEYRKLAAIMITDMAGYSDLARRDEEGALRALDLYRRIVRD
                     AVASHGGTEVKTMGDGFLLEFPSALDAVRCAVQIQQRLRDLNAQRGPNEPIGVRIGVH
                     LGEVIHKESDILGDGVNIAYRIEPLAEPGGIWLSEDVARQVEAKVAWPLRRLGTAELT
                     GPSILIGIYRVVLPWEAPAAGEADAPPGRRCPRLILGLVAVCLILAVAAGSLWLTRGR
                     GGRGLGPAGTAPLSPAKPMAPSGAPPAKTATAPTPGSAVPEAYRDYLAGRQIWADNAE
                     PGGCKEAAAHFAEATQKDPRFAEAWAALARCYALMGAYGQSAPSDAFVKARAAAAKAL
                     SLDPQSAEALIALALVQGCHEGNWQEAGASMQKALSLASGSAYAHDAQAYWLALEGRA
                     AESAAESAEAQKIDPVNRSLPLNAGYLALAAGDAPRALGLVAALASQSGTSPQALTLL
                     ARAQWASGNLAEASATLAGVPQPALLPPTFAVLSFLVRAPGGDAASASQALAALEQRA
                     ASGEYVSAALIAACHAAVGNRPQAQAWLERARTERSIELLFAHTWPELAPIRNDPALA
                     PVLLNAGGG"
     gene            complement(1947..3032)
                     /locus_tag="PLQ54_08535"
     CDS             complement(1947..3032)
                     /locus_tag="PLQ54_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOS93345.1"
                     /translation="MPAAQSTAQAVAWCVALLCLPGCGAERVQVLPPSTPDRLVLDPV
                     RLTADDLVQRLSFFSGPSSALHSDPGPEEESIAEANSYLAAHLTSGSGYFVQISDPHI
                     GGGVDGSPKGFDALFSRATPVPRFQRVLREIARLSPLPEFIVITGDLSDTGARQELMQ
                     CRDRLPSSGPPIYLVRGNHDADLNTFMHVFGELPYIDADRAAEAGFCYTFDALGAHFV
                     VLDSETISPNGAELEWLDGDLAAHAGQPCVVFGHRHLVHVDPIIEAYLGLAQPYADDL
                     LGVLGRHAGVGWFFAGHIHLNSLKALGELRLATITSTFYDLSDGRCVAPGYAGLFAHL
                     VCLRDGQVQWVALKEFGEHTRTIWPTG"
BASE COUNT          527 a         1068 c         1117 g          455 t
ORIGIN      
        1 tccgtgccat gtagcccgcg gccaagtgct caccggccac atggagcgcc tcggtgaggg
       61 tgccgcccgc catcggggcc acccgatctt gatgacgcgg agccaccggc accgcatcca
      121 ccgcctctca ccgcatccct ctcccgcagg aagcgggacc tcgaagccta tcccccgcca
      181 gcgttcagca gaaccggggc cagcgccggg tcgttgcgta tgggcgcgag ctcgggccag
      241 gtgtgggcga agagcagctc gatggaacgc tcggtccggg cccgttcgag ccaggcctgt
      301 gcctgcggac gattgcccac ggcagcgtga cacgcggcga tcagggcggc ggacacgtac
      361 tcgccactgg cggcgcgctg ctcgagtgcc gccaaggcct gactggccga tgcggcatcg
      421 cccccggggg cacggaccag gaatgatagt accgcgaagg ttggcgggag cagagcgggt
      481 tgcggaacgc cggccagcgt ggccgaagct tccgccagat tcccgctcgc ccactgggct
      541 cgcgccagga gagtcagcgc ctggggactg gtgcccgact gcgaagccaa cgcggcgacc
      601 aggcccaggg cccgcggggc atcaccggcg gccagggcca ggtagcccgc gttgaggggg
      661 agcgagcggt tgacgggatc gatcttctgg gcctcggcgc tctcagcggc gctttcggca
      721 gcccgccctt ccagagcgag ccagtaggcc tgtgcgtcgt gcgcgtacgc gctccccgat
      781 gccaggctga gggctttctg catggacgcg cccgcctctt gccagttgcc ctcatggcac
      841 ccctggacca gcgccagggc gatcagcgcc tcggccgact gggggtcgag gctcaatgcc
      901 ttcgcggcgg ccgcccgggc tttgacgaat gcatcggaag gcgcgctctg cccgtaggcg
      961 cccatgaggg cgtagcatcg cgcgagagcc gcccacgcct cggcgaaacg tgggtccttt
     1021 tgggtcgctt cggcgaagtg ggccgcggcc tctttgcagc cgcctggctc cgcgttgtcg
     1081 gcccaaatct gcctcccggc caagtagtcg cggtaggcct cgggaacagc ggaccctgga
     1141 gtgggggcgg tcgccgtttt ggccggtggc gcccccgacg gagccatcgg cttcgccggc
     1201 gacaggggtg cggtgccggc cggccccaag cccctgccgc cgcggccccg ggtgagccac
     1261 agggatccgg cggcgacggc cagaatgagg cacacggcca ccaggccgag gatgagccgc
     1321 gggcagcggc ggcccggcgg cgcgtccgct tcgcccgcag ccggcgcctc ccaggggagc
     1381 acgacgcggt agatgccaat gaggatggag ggaccggtca gctcggcggt acccagccgc
     1441 cgaagcggcc aagcgacctt ggcctcgacc tgccgggcca catcctccga gagccatatc
     1501 ccgcccggct cggccagggg ttcgatgcgg taggcgatgt tcacgccatc gcccaggata
     1561 tcgctctcct tatgtatgac ctcgcccaga tgcacaccga tgcgcacgcc gatgggctcg
     1621 ttgggtcctc gctgagcatt gagatcgcgc agccgttgct ggatctggac cgcgcatcgg
     1681 acggcatcca acgcgctggg gaactcgagc aggaaaccat cgcccatggt cttgacttcg
     1741 gttccgccat gggaggcaac ggcgtcacgg acgatacggc gatagaggtc cagcgcgcgg
     1801 agcgcgccct cctcgtcgcg acgagccagg tcggagtagc ccgccatgtc cgtgatcatg
     1861 atcgccgcga gcttgcggta ctcgaggtcc agcaccggca accacccagt cctcgcgctg
     1921 catcgagcag atgtctctgt accagcctac cccgttggcc agatcgtgcg cgtgtgctcg
     1981 ccgaactcct tcagggccac ccattgtacc tgcccatcac gcaggcacac caggtgggcg
     2041 aagaggccgg cgtagccggg ggccacgcag cgcccgtcgc tcaggtcata gaaggtcgac
     2101 gtgatcgtcg ccaagcgcag ctcgcccagg gccttcagcg agttcaggtg gatgtggccg
     2161 gcgaagaacc agcccacgcc ggcgtggcgg cccagcacgc ccagcaggtc gtctgcataa
     2221 ggctgcgcca agcccaggta cgcctcgatg atgggatcga catgcaccag atgccgatgg
     2281 ccaaacacga cgcatggctg gccggcgtgg gccgccagat ccccgtcaag ccactcgagc
     2341 tccgcgccgt tgggggaaat ggtctccgag tcgagcacga cgaagtgggc acccagcgca
     2401 tcgaaggtgt agcagaagcc cgcctcggcg gctcgatctg catcgatgta gggcagctcg
     2461 ccgaagacgt gcatgaaggt gttgagatcc gcatcatggt tgccgcgcac gaggtagatg
     2521 ggcggcccgc ttgatggcag ccgatcacgg cactgcatca gctcctggcg ggcgccggtg
     2581 tcggacaggt cgcccgtgat gacgatgaac tcgggcagcg gtgacaggcg ggcgatctcg
     2641 cgaaggacgc gctggaatcg tggaaccggt gtcgctcgac tgaagagggc gtcgaacccc
     2701 ttgggagagc catcgacccc gccgccgatg tgtggatcgg agatctggac gaagtagccc
     2761 gaccccgatg tgaggtgggc ggcgaggtag gagttggcct cggcgatgct ctcctcctcg
     2821 ggacccggat cggagtggag cgccgacgag ggacccgaga agaagctcaa tcgctggaca
     2881 aggtcgtctg cggtaaggcg gaccggatct agaaccaggc ggtcgggcgt cgagggcgga
     2941 agcacttgga cgcgctcggc gccgcacccg ggcaggcaga gcagggccac acaccaggca
     3001 acggcctgtg ccgttgactg agcagctggc atcccgtggc gcttctgcaa gggaagcccc
     3061 cgtatggcag cagacgccta gaccggcgtc gagcccacct gacggacatc gacgaaccgg
     3121 ccggtctcgg ccgactcctt ggcagccagg caggtataga cgctctg
//
LOCUS       DAOSPL010000410         3166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_30609_length_3166_cov_3.608807, whole genome shotgun sequence.
ACCESSION   DAOSPL010000410 DAOSPL010000000
VERSION     DAOSPL010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30609_length_3166_cov_3.608807"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..839)
                     /locus_tag="PLQ54_08540"
     CDS             complement(90..839)
                     /locus_tag="PLQ54_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93346.1"
                     /translation="MPCASFALTVSASKRLIAKAVAQLPCVRHALAHSIVAISKGTTN
                     GYVVEEILGRPIDKAQYVLGTTQPQVHRKPMTDQRMADVILERGTVVEDTSVVDVIAQ
                     MGEGDVLIKGANALSADRKVAGILVGDPQGGTWGKTCGRTGGSFVTRVIPVGLEKVIA
                     GDVLSAAATLQSGTGRRGATPNLMPVQGVIVTEIEAIELLAGVSATQIGAGGINGAEG
                     AVWLFVEGSERAVARARELVEAVQGEPPFVS"
     gene            complement(856..1656)
                     /locus_tag="PLQ54_08545"
     CDS             complement(856..1656)
                     /locus_tag="PLQ54_08545"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOS93347.1"
                     /translation="MRDERASAPTGMPAPTRAGEAMSAERYIEKVQTSIQLVLETQRE
                     PIAEAARILVTAIRDGKRLYSFGCSHSFILTEELVYRTGGLMLVNPIYPHGMNLSVRP
                     MTMTSQLERLEGLGNILLRNSGAGQGDALLLASTSGRNAVAIDMALEAKARGVSVIGI
                     TSMAYSRSVSSRHSSGKKLYEIADVILDNCVPAGDAAVQFEGFAQAVGPLSTVTGVAI
                     VNALVCQVVQELIEAGVTPPVFVSANLDGGDEHNARLLAENADRILYM"
     gene            complement(1693..2427)
                     /locus_tag="PLQ54_08550"
     CDS             complement(1693..2427)
                     /locus_tag="PLQ54_08550"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HOS93348.1"
                     /translation="MLALDYLNQLRALLDRIETTQLQKIDQAAEMIADSLANGGSFLI
                     HDSGHMLNSELIHRAGGLMALRAFTFGLSINAAVPAATARKVEPLDQRDDYFDLIIDA
                     SPLKAGDVLIIGSVSGRNPAQVGLALAARRRGIKLIAITSLSYSQSVTSRHHSGQRLF
                     EVVDIAIDNCGVLGDACVAVEGLPVKALPTSGIGAATVAWLICAQVIERLLARGIRPH
                     VYKSVNTDDGADFNARETEDFEKTGI"
     gene            complement(2477..>3166)
                     /locus_tag="PLQ54_08555"
     CDS             complement(2477..>3166)
                     /locus_tag="PLQ54_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOS93349.1"
                     /translation="EWDAILAASRLEYGLVVLAGTGSGVFARNREGTAVHMGGSGPVI
                     GDEGSAYDTGVRGLRACVRADYSQARSTSLRAAVLAEVGVTAPHELIDWVYARPASRR
                     DIAALARVVDREAQAGDAVACGILANAAAALADLALEALAVASMLDEPYAMVPAGSVA
                     TRSRGFRQALADRVCQGAPLIEPKPPGLSPAVGAGLLALRDLGVPWTDSLYARARETA
                     AGFEEPPPNNL"
BASE COUNT          510 a         1042 c         1066 g          548 t
ORIGIN      
        1 agccaggatc cagagccccg ccagcagaga gactcccagg cgatgttttg tatgcaatac
       61 catcacttac cactcctcct cgcgtgaacc tacgagacga acggcggctc gccctggacc
      121 gcctcgacca gctcccgggc ccgagctacg gcacgctcgc tgccctcgac gaacaaccac
      181 actgccccct cggcgccgtt gatcccgccg gcgccgatct gggtcgccga cacgccggcg
      241 agcaactcga tggcctcgat ctcggtgacg atgaccccct gaacgggcat gaggtttggt
      301 gtggcgcccc gccggcccgt ccccgactgc agcgtcgcag ccgccgagag cacgtcgccg
      361 gcgatgacct tctcgagccc gacgggtatg acccgggtca cgaaggaccc acccgtacgg
      421 ccacaagtct tgccccatgt gccgccttgg gggtcgccca ccagaatgcc ggccaccttg
      481 cggtcagccg agagcgcgtt ggcccccttg atcagcacgt ccccctcgcc catctgagcg
      541 atcacgtcga ccaccgacgt atcttctacg acagtgccgc gctcgagtat cacgtcggcc
      601 atgcgttggt cggtcatcgg tttgcggtgc acctgtggct gggtggtacc gaggacatac
      661 tgcgccttgt cgatgggccg tccgaggatc tcctcgacca catatccgtt cgttgtgcct
      721 ttgctgatgg ccacgatgct gtgggccagg gcgtgccgga cgcacgggag ttgggcgacg
      781 gctttggcga taagccgttt cgaggcggag acggtcagcg cgaacgacgc acaaggcaca
      841 gagacttcct cctcactaca tgtaaaggat ccgatcagcg ttctcggcca ggaggcgcgc
      901 gttgtgttca tcgccaccgt cgagattggc gctgacgaac acgggaggag tcactccagc
      961 ctcgatcagc tcctgcacca cctggcagac gagcgcgttg acgatggcaa ccccggtgac
     1021 cgttgagagt ggtcccacgg cctgggcaaa gccctcgaac tgcactgccg catcgccggc
     1081 gggcacgcag ttgtcgagga tgacgtcggc gatctcgtac agcttcttcc cgctggagtg
     1141 ccggctgctc accgagcggg agtaggccat cgacgtgatc ccgataacgg atacacctct
     1201 cgccttggct tcgagggcca tgtcgatggc cacagcgttg cggccggagg tcgatgcgag
     1261 cagcagggcg tcgccctggc cggcccccga gttcctaaga aggatgttcc cgaggccctc
     1321 gagtcgctcg agttgggagg tcatcgtcat gggccgcacg ctcaggttca tgccgtgcgg
     1381 gtagatgggg ttgacgagca tcagcccgcc ggtgcggtag accagctcct cggtcaggat
     1441 gaacgaatgg ctgcatccga aggagtagag gcgcttgccg tcgcggatcg ccgtgaccag
     1501 gatccgagcg gcctcggcga tgggctcccg ctgtgtctcg agtacgagtt gtatggatgt
     1561 ctgtaccttt tcgatgtacc gctcggcgga catggcttcc cccgctctcg tcggcgccgg
     1621 cattccggtc ggcgctgacg cccgctcatc gcgcacgagt ctcctcctgt ggcggcgcgg
     1681 cgtccccagg ctctagatcc ccgttttttc gaagtcttcc gtctcgcggg cgttgaagtc
     1741 cgcgccgtca tctgtgttga cactcttgta tacgtggggc cgtatccctc gggccaggag
     1801 cctctcgatc acctgggcgc agatcagcca tgccacggtt gccgcgccaa tgccggacgt
     1861 ggggagggct ttcaccggga gaccctcgac ggcgacacag gcatcgccca gcacgccgca
     1921 gttgtcgatg gcgatatcga cgacctcgaa cagccgctgc ccactgtggt gccgtgacgt
     1981 gaccgactgg gagtaggaga gtgaggtgat ggcgatgagc ttgatccctc ggcgcctggc
     2041 ggccagggcc agaccgacct gtgccgggtt ccggccggat actgagccaa tgatgagcac
     2101 gtcaccggcc ttcagtgggc tggcatcgat gatgaggtcg aagtagtcgt cgcgctggtc
     2161 gaggggctca accttgcggg cggtggctgc gggcacggcg gcgttgatgc tcaggccgaa
     2221 ggtgaaagcg cggagagcca tcagtcctcc ggcgcggtgg atcaactcgg agttgagcat
     2281 gtgtccgctg tcgtggatca ggaacgaacc tccgttggcc agggagtcgg caatcatctc
     2341 cgcggcctgg tcgatcttct gaagctgggt cgtctcgata cgatccagca gagcgcgtaa
     2401 ctggttgaga tagtcgagcg cgagcatcga tttggctccg tctccggtcg gtgtggcagc
     2461 ccgttggcaa ggggctctag aggttgttcg gcggtggctc ttcaaagcct gcggccgtct
     2521 ctcgggcccg ggcgtagagc gagtcggtcc acgggacacc cagatccctg agtgccagca
     2581 gccccgcccc gacagccggc gagaggcccg ggggcttggg ctcgatcagc ggggcgccct
     2641 ggcacacgcg atcggccaac gcctgacgga atccgcgcga gcgggtggcc accgagccgg
     2701 cgggcaccat ggcgtagggc tcgtcgagca tcgacgcgac ggccagggcc tcgagtgcca
     2761 gatcggccaa ggccgcggcc gcgttcgcga ggatcccgca tgcgacggca tcgccggcct
     2821 gggcctcccg gtcgacgact cgagccaggg cggcgatgtc acgccgagag gcggggcggg
     2881 catagaccca gtcgatgagc tcgtgcgggg ccgtcacgcc cacctcggct agcacggccg
     2941 cacggagcga tgtggatcgc gcttgggagt agtctgcccg cacgcaggcg cggaggccgc
     3001 ggacgccggt gtcgtaggca ctcccctcgt cgccgatgac gggccccgag ccacccatgt
     3061 ggaccgcggt accctctcgg ttgcgcgcga acacgccgct gcccgttccc gcgagcacca
     3121 cgaggccgta ttcgaggcgg gaggcggcca ggatcgcgtc ccattc
//
LOCUS       DAOSPL010000411         3163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_30659_length_3163_cov_3.549550, whole genome shotgun sequence.
ACCESSION   DAOSPL010000411 DAOSPL010000000
VERSION     DAOSPL010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30659_length_3163_cov_3.549550"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLQ54_08560"
     CDS             complement(<1..255)
                     /locus_tag="PLQ54_08560"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOS93350.1"
                     /translation="MLVIVAVLGLAGVRGTEPSVRLVAVGDVLLDRGVAAVAAQDGWG
                     SILGRVGDLLGTADIAYANLECPVTHRYDPVPKKARFRAEP"
     gene            complement(257..2479)
                     /locus_tag="PLQ54_08565"
     CDS             complement(257..2479)
                     /locus_tag="PLQ54_08565"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HOS93351.1"
                     /translation="MRCLAVVLALVLAGLTWWLAVRDGLGVSQALAQDEPEAEAQAGA
                     GDDDGTALFSGEALRRPPADFGTVRAEVKPYRVTATLNTVGNLRAFTKARPLSTDERS
                     MLARNLFVVTPTDQLQLFYIYENNDYLNLPSFVTVDAALQVHHEVFDYVLRTTEAERL
                     LPVLEGFVSGVAAKTAELAAGPAGPSAAWNEACQRNAALIEVAARLLDRPTGNELPAS
                     VTAWVEAEMTLLGAHDADSMSPILGYKFDYTQFIPRGHYVRSDDLKRFFLAMMWLGRA
                     GLPTRDLDGNVLEVPVLQSLMLSEMVRDEAIRAKWQAIYEPTAQLVGIADDLTPIEWD
                     DTRARVGGKDSRARDFADPGKLSRFVADVERVRPPRVLSHPGGPNAASQVCQFRLFGQ
                     RYTPDGEVFSRVTHATERPFPVGLDAMAALGSERAAAILDADPAEYNPGGWAAYVPRR
                     QEITAEFAAMSPEFWTKNVYCGWLNALRALFVALPEGYPSFMRSQAWQDKSANTALAS
                     WAELRHDTILYAKATTAECGDEPPPELRGYVEPNVVLYDRLLRSTAKTYEVLRAHDLL
                     TDAVVERFTLAEDLLTFLISVSEKELRNEPLTPQEYEKIRYLGSDIEWLTLMSVGADV
                     SSWSDMSESDQDMAVVADVHSGYGFALEEAVGRANEILVIVPIEGKLTLTRGACLSYY
                     EFKEPASNRLTDQQWRERLSAGQAPPPPKWTSSFLLPAEKSGAKGVNPEEYKAYSSGC
                     "
     gene            complement(2476..3117)
                     /locus_tag="PLQ54_08570"
     CDS             complement(2476..3117)
                     /locus_tag="PLQ54_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOS93352.1"
                     /translation="MPGPHRPSVARTCAALALSLAAAYPWRTPELAWPEAVQTDLDGD
                     GTVETLWFDRDADQTVRLCRPGRPDWVGIPHSLKPWKMVVADVEGDGVLEVAIGVHKP
                     TPYRPFPHNCLYVYGVNREGLFPKWRGSALSHPFTNYTFAEQDGTPGDELVALETDLD
                     GLRCVTVYHWKGFGFAGETLPGRWREAELVETGHGGVTVRAEGRWVDIPGGAR"
BASE COUNT          509 a         1073 c         1055 g          526 t
ORIGIN      
        1 tggctcggcg cggaagcgcg ccttcttcgg gaccgggtcg taccggtggg tgaccgggca
       61 ctcgaggttg gcataggcaa tgtccgccgt gccgaggaga tcccccaccc gaccgaggat
      121 gctgccccag ccgtcttggg cggcaaccgc ggccaccccg cggtcgagca gcacatcgcc
      181 gaccgccacc aggcgcacgc ttggctcggt tccccggaca ccggcgaggc caagcaccgc
      241 cacgatcacc agcacgctag cagccgctgc tgtaggcctt gtactcctcc ggattgacac
      301 ccttcgcacc actcttctcg gcaggcaaca ggaaactgct ggtccacttg ggaggaggag
      361 gggcctggcc cgcgctcagg cgctcgcgcc attgctggtc agtcagccgg tttgaggctg
      421 gctctttgaa ctcgtagtag ctcaggcatg cgccccgcgt cagtgtgagc ttgccttcaa
      481 tgggcacgat gaccaggatc tcgttggcgc gcccgacagc ctcctccagt gcaaagccgt
      541 agcctgagtg cacgtcggca acgactgcca tgtcttggtc ggactcgctc atgtcggacc
      601 atgaactgac gtcggcgccg accgacatca gagtcagcca ctcgatgtcc gatccgaggt
      661 atcggatctt ctcatactcc tgcggggtca gcggctcgtt tcgaagttcc ttctccgaga
      721 cagagatcag gaacgtgagc aggtcctccg ccagtgtgaa gcgctcgacg accgcgtcgg
      781 tgagcaggtc gtgggctcgg agtacctcgt aggtcttggc tgtggaccgc agtagccggt
      841 cgtagagcac cacgttcggc tccacgtagc cgcgtagctc cggtgggggc tcgtcgccgc
      901 actcggcggt agtcgccttt gcgtagagaa tcgtgtcatg gcgcagttcg gcccacgagg
      961 ccagcgcggt gttggcggac ttgtcctgcc acgcctggct ccgcatgaat gaggggtacc
     1021 cctcgggcag ggcgacgaac agggcccgca gggcgttcag ccagccacag tagacattct
     1081 tcgtccaaaa ctcgggtgac atggcggcga actcggcggt gatctcctgg cgtcggggga
     1141 cgtaggccgc ccagcccccg gggttgtact cagccgggtc ggcatcgagg atcgcggccg
     1201 cccgctcgct gcccagtgcc gccatggcgt ccaggccgac ggggaagggg cgctcagtcg
     1261 cgtgcgtgac ccggctgaag acctcgccgt cgggcgtata gcgctggccg aagagccgga
     1321 actggcatac ctggctcgcc gcattcggcc cacccggatg cgagagcacc ctgggtggcc
     1381 gcactctctc gacatcggcg acgaaacggc tgagcttccc cgggtccgca aagtcccggg
     1441 ctctgctgtc cttgccaccc acacgggcgc gggtgtcgtc ccactcgatg ggagtgaggt
     1501 cgtcggcgat gcccacgagc tgcgcggtag gttcgtagat ggcctgccac ttggctcgga
     1561 tcgcctcgtc gcgcaccatc tcgctcagca tcaggctctg caggacaggc acctcgagga
     1621 cattcccgtc caggtcgcgc gtcggcagcc cagcccttcc gagccacatc atggcgagga
     1681 agaatcgctt gagatcatcg ctccgcacgt aatggccgcg ggggatgaac tgggtgtagt
     1741 cgaacttgta gccgagtatg gggctcatgg agtcggcgtc gtgggcgccc agcagcgtca
     1801 tctcggcctc gacccaggcc gtgaccgacg cggggagctc gttcccggtg ggtctgtcga
     1861 ggagccgcgc ggccacctcg atgagggccg cgttgcgctg gcaggcctcg ttccatgccg
     1921 cggacggtcc ggccgggccg gctgccagtt ctgcggtctt cgccgccaca ccggagacga
     1981 acccctcgag cacgggcagg agtctctccg cctcggtggt ccgcagcacg tagtcgaaca
     2041 cctcgtggtg gacctgcaga gccgcatcga cggtgacgaa cgacggtagg ttgaggtagt
     2101 cgttgttctc gtagatgtag aagagctgca actggtcggt gggggtcacg acgaacagat
     2161 tccgggccag catacttcgc tcgtccgtac tcagcggtcg ggctttggtg aaggccctga
     2221 ggttcccgac cgtgttcagc gtcgcggtca ccctgtatgg cttgacctcg gctcggaccg
     2281 tgccgaagtc ggcgggggga cggcgcagcg cctcgccgct gaacagagcc gtcccatcat
     2341 catcgcccgc gcccgcttgg gcctcggcct ccggttcatc ctgcgccagc gcctgcgaga
     2401 cgcccagacc gtccctgacc gccagccacc acgtcagccc cgcgagaacc agagcgagca
     2461 ccacagccag acacctcatc gcgcacctcc tgggatatcg acccagcggc cctccgcccg
     2521 cacggtgact cccccgtgcc cggtctcgac gagttcggcc tcgcgccagc ggccgggcag
     2581 cgtttcaccg gcgaacccga agcccttcca gtggtagacc gtgacacacc gcaagccatc
     2641 gaggtcggtc tcgagcgcca cgagctcatc gcccggcgta ccgtcctgct cggcaaaggt
     2701 gtagttggtg aacgggtgac tcagcgccga gccgcgccac ttggggaaca ggccctcgcg
     2761 gttcaccccg tagacgtaca ggcagttgtg aggaaacggg cggtagggag tgggtttgtg
     2821 aactccgatg gcaacctcga ggaccccatc gccctcgaca tcggccacga ccatcttcca
     2881 cggcttgagt gagtgcggga tacccaccca gtcgggccgc ccgggccggc ataggcgcac
     2941 ggtctggtcc gcatcgcggt cgaaccagag cgtctcaacc gttccgtcgc catccagatc
     3001 ggtctggacg gcctcgggcc aggcaagctc tggcgtccgc caggggtacg cggcggccaa
     3061 ggagagagcc agagcggcgc aggttcgggc cacgctcggc cggtggggtc ccggcatggg
     3121 tgcacctccc cgcggcgcac agtccggccc agacgggcag agg
//
LOCUS       DAOSPL010000412         3158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_30725_length_3158_cov_3.307444, whole genome shotgun sequence.
ACCESSION   DAOSPL010000412 DAOSPL010000000
VERSION     DAOSPL010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30725_length_3158_cov_3.307444"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2347
                     /locus_tag="PLQ54_08575"
     CDS             <1..2347
                     /locus_tag="PLQ54_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93353.1"
                     /translation="EADLGAAPAIRRVEERFATQQALVRAIGAIDARAAGPVVDQALE
                     SARALRPAARGLEARVVLAQSVAAFDRSRAVALLDHVVVDCDALPAAELPVTDRLSVL
                     QNVAAALFGLDPSRAWGVLEDILRPTPGGGDAPWVPEPALWDRAVGVDAARTAECARR
                     SAVPALSLGRVAEALCIRDPAQASALGAEALRLAGDSADLCDEVAGRLAPRLAPTSSQ
                     GALSMARTVGPTRRDAVLAETLAAAPPDAAAGCLDLAQSVVREAERQVALAAVISGVA
                     TQDPDRALELASGFAADERLLADVLIALAPLAPGAARARLGTLHDARSLDEVRAALAL
                     ALARRGTPGGEEVAEGITDAGLRARCLADMSEGVPALAAQAAALAASLPDQRAQAAAC
                     AYVAHGCAGNGRVAAAAADRAFALASELGVGAFAPAAWCELVADLAAASPDQAMALAG
                     LSEPADTAAWAYSRVAGALAGSDSERAARARQRAMEVAARIEDAYERSQRLTACVTAW
                     WATAPEAARSAAQAALEAAFAVPEPVEQARARADVSIAMARLDPSTAQSWAYAVGALD
                     AVEAIRALMTIGEVASEQNPLGPSFPYDQAAHLAEQIGPNEDLDAGQAAALRVRQLLL
                     VARELSAVPGAGGRSRELAEQASAALKALGPDVEPELAATAARDAAVVAFRLDPANGL
                     AAVEKLPAPIRAETLCALASGPPARADLLDRAIEAVRQVTDRDEADRLMGGLISSVGP
                     TVPDRVKALAALLPDDMARADVLLRVSKALASGVGQSTPQG"
     gene            2382..>3158
                     /locus_tag="PLQ54_08580"
     CDS             2382..>3158
                     /locus_tag="PLQ54_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOS93354.1"
                     /translation="MGEPVLLGLDLGGTKISAVAGTPDGRIIRKAVVPTRGEEGPERV
                     VSRIIDLLRLVRESAQVSAASVAVLGVSHGGPVDPRTGLCSSPNIPGWRDIDLGSLLG
                     DALSSPVALDNDANAGAVAEFLYGAGRGLRHICYMTMGTGIGGGIVSDGRVFRGAFGM
                     AGEIGHVTIVRDGRECSCGRRGCLQAYASGPAIEAIAAERAAADHDTELLPLVDSVGR
                     IPCEKLCELARQGNANARAVLQEAAAAMGTGVANLIKVVDP"
BASE COUNT          393 a         1024 c         1265 g          476 t
ORIGIN      
        1 ggaggccgac ctgggcgcgg cgccggcgat ccggcgcgtc gaagagcgtt tcgcgacgca
       61 gcaggctctc gttcgggcca tcggggccat agacgcgcgt gccgcgggac cggtggtaga
      121 tcaggcactg gagtccgccc gcgcgttgcg gccggcggcg cgcgggctcg aggcgcgcgt
      181 ggtcctggcc cagtcggtgg cggctttcga tcgctcgagg gcggtcgccc tgctggacca
      241 cgtggtcgtg gactgcgacg cgctacctgc cgccgagttg ccagtcaccg accgcttgag
      301 cgtgctgcag aacgtcgcgg cggcgctgtt tgggctggat cccagccggg cgtggggcgt
      361 gctggaggac attctacgtc cgacgcccgg cgggggcgac gctccgtggg tgcccgaacc
      421 agccctctgg gatcgggccg ttggcgtgga tgccgcgaga acggcggaat gcgcccggcg
      481 atcggccgtg cccgcgctga gcctcggccg ggtcgctgag gccctgtgca tccgtgaccc
      541 ggctcaagcg tcggcactgg gcgccgaggc gctccggttg gccggcgact cggcggacct
      601 gtgtgacgag gtggctggcc ggctggctcc acgtctggcc ccgacgagct cgcagggcgc
      661 tctgtcgatg gcgaggaccg tgggaccgac gcggcgcgac gccgtgctgg cggaaacgct
      721 ggcggcggcg ccgcccgatg cagcagcggg atgcctcgat ctggcgcagt ccgtcgtccg
      781 cgaggccgag cgccaggtcg cgctggccgc cgtgatctcg ggcgtcgcga ctcaggaccc
      841 cgatagggcc ctggagctgg cctcaggctt tgcggccgac gagcgcctgt tggccgacgt
      901 gttgatcgcc ttggccccgt tggcgccggg ggcggcccgg gcgcgtctcg gtaccctgca
      961 cgacgcgcgg agcctggacg aggtgcgggc cgcgttggcg ctggcgttgg cacgccgggg
     1021 aacgccgggc ggggaggagg tcgccgaggg gatcaccgac gcgggtctgc gggcccggtg
     1081 cctggccgac atgtccgagg gggttcccgc gctggcggcc caagctgccg ctctcgccgc
     1141 cagcctaccc gaccagcgtg cgcaggcagc ggcgtgcgcc tatgtggccc atgggtgcgc
     1201 gggcaacgga cgggtggcgg ccgcggctgc cgatcgggct ttcgccctgg cgtcggagct
     1261 cggcgtgggc gccttcgcgc ccgcagcctg gtgtgagctg gtggccgatc tcgcggcggc
     1321 ttctcccgac caagccatgg cgttggccgg cctgtcggag cccgccgaca cggccgcatg
     1381 ggcctactcg cgtgtcgccg gggcgttggc cggctccgac tcggagaggg cggcgagggc
     1441 tcggcagcgg gcgatggagg tggcggcgcg catcgaggac gcctatgaac gcagtcagcg
     1501 cctcacagcc tgcgtgactg cctggtgggc cacggccccc gaggccgctc ggtcggcagc
     1561 gcaggcggcg ctcgaggcag ccttcgccgt gcccgagccg gtcgagcaag ctcgagcccg
     1621 agcggatgtc tccatcgcga tggctcggct ggacccgagc accgcgcaga gctgggccta
     1681 cgcggtgggg gctctcgacg ccgtcgaggc aatccgggct ctcatgacca tcggcgaggt
     1741 ggcgtctgag cagaaccccc tggggccgtc cttcccctac gatcaggcgg cgcacctggc
     1801 tgagcagatc ggcccgaacg aagacctgga tgccggacag gcggctgccc tgcgggttcg
     1861 gcagctgctg ctggtggccc gggagctgtc tgcggttccc ggggcgggag ggcgctcgcg
     1921 ggagctggcg gagcaggcgt cggcggcgct caaggcgctg ggcccggatg tcgagcccga
     1981 gctggcagcc acggcggcgc gggacgcggc ggtggtcgca ttccggctgg accccgcgaa
     2041 tgggctggcc gcagtggaga agctaccagc ccccatccga gctgagacgt tgtgcgccct
     2101 ggcttccggg ccgccggccc gggccgatct gctggaccgg gcgatcgagg cagtccggca
     2161 ggtcacagac cgcgacgagg cggatagact gatggggggc ctgatcagtt cggtcggtcc
     2221 gacggttccc gaccgggtga aggccctggc ggctttgctg cccgatgaca tggcgcgggc
     2281 agacgtgttg ctgcgggttt cgaaggccct ggcatccggg gtcgggcaga gcacgccgca
     2341 gggctagccg gccgcagctc aggggagagg gttcaggagc attgggtgaa ccagtactgc
     2401 tcgggctcga tttgggtggc acgaagatct cagcggtggc ggggacaccg gatggccgca
     2461 tcatccgcaa ggccgtggtg ccgactcgcg gcgaggaggg gcccgagcgt gtcgtgagtc
     2521 gcatcatcga tctgctccgg ctggtgaggg agtcggccca ggtgtcggcc gcgtcggtcg
     2581 ccgtcctggg ggtgtcgcat ggaggtccgg tggacccgcg cacggggctt tgctcgtcgc
     2641 ccaacatccc cggctggcgc gacatcgact tgggctcgct gttgggcgat gccctgagct
     2701 ccccggtggc gctggacaac gacgcgaacg ccggcgccgt ggccgagttt ctctatggtg
     2761 ccgggcgggg actccggcac atctgctaca tgaccatggg cacgggcatt ggtggaggca
     2821 tcgtatcgga tggccgggta ttccgcggcg cgttcggaat ggcgggcgag atcggtcatg
     2881 tcaccattgt ccgtgacggc cgggagtgca gctgtgggcg gcgcggatgt cttcaggcct
     2941 atgcgtcggg acctgccatc gaggcgatcg ccgccgagag agcggccgcc gaccatgaca
     3001 ccgaactgct ccctctggtg gacagcgtgg gccggattcc gtgtgagaag ctctgcgagc
     3061 tggctcggca ggggaacgcc aatgcgcggg cggtcctgca ggaggcggca gcggccatgg
     3121 gaacgggtgt cgcgaatctg atcaaggtgg tcgatccc
//
LOCUS       DAOSPL010000413         3153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_30790_length_3153_cov_2.785991, whole genome shotgun sequence.
ACCESSION   DAOSPL010000413 DAOSPL010000000
VERSION     DAOSPL010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30790_length_3153_cov_2.785991"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1305)
                     /locus_tag="PLQ54_08585"
     CDS             complement(136..1305)
                     /locus_tag="PLQ54_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002537795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS93355.1"
                     /translation="MPGKRRVLVINHYAFPREYGGLTRNFDMFSRLKNWQFRIIATSR
                     NHYTGEVMVVKDPRWTLLSIPSYSGNGSRRILGWILFAIKATLKALRTRADVYFGSSP
                     HPLAAAGAMVVAAVRRRPFVLEVRDLWPESIVTGGTLRAGSGVHTALVWLERLLYRRA
                     AKIVVVTKGWENHFESLGIDIGKVTVISNGADLADYDVPDSKDALRQRYGISGFTAVF
                     TGNHSPYVGLELILDAAAALPDVNFVCVGNGSRKAWAISESANRGLTNVEFRDPIGKP
                     ELPWLLKACDVGLHTISPQSVFDKGMSPNKLFDYMASGIPIVSNARIPLRDVVKDDQA
                     GPVVEPDELAQGIARVRDADPQTRVRWSQEGRRIMEDRFSLRAAAARLEAVLDSV"
     gene            complement(1307..2368)
                     /gene="wecB"
                     /locus_tag="PLQ54_08590"
     CDS             complement(1307..2368)
                     /gene="wecB"
                     /locus_tag="PLQ54_08590"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOS93356.1"
                     /translation="MRILSIVGARPQFVKLGPIARAFRERSDIDHVIVHTGQHYDPLL
                     SDVFFSDLGIGKPHVHLGVGSGSHGEQTGAILSALDSVLSEYAPDWVLVYGDTNSTLA
                     GAVAAVKLHLPLAHLEAGLRSFNRRMPEEHNRVLTDHAADLLLAPTQVAMQHLKDEGL
                     SSRARLVGDVMTDVCFATRDAVANQNPTLPEGVLGDYVVATIHRADNTDDPDRLSAII
                     EALAVLSVPVVLLTHPRLRAKASAFGIPLTQGSIRPTDSLAYPQMLRTVQCAAGVVTD
                     SGGLQKEAFLLQTPCTTIRTETEWVETLADGWNVLDPTLALVREAAARPRPTAKQAMP
                     YGEGKAAELVAQTLVQEGR"
     gene            complement(2391..>3153)
                     /locus_tag="PLQ54_08595"
     CDS             complement(2391..>3153)
                     /locus_tag="PLQ54_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOS93357.1"
                     /translation="FAGHRPDVVFHAAALKHLPMLEQYPLEGWKTNTLGTLNVLRAAE
                     RYGVERFVNISTDKAADATSVLGKTKRLAEELTAYFATRTNRTFLSVRFGNVLGSRGS
                     MLHTFTAQIDAGGPLTVTHPDITRFFMTIPEACELTIQAGAIGNPGDVLVLDMGEPVK
                     ILDVARGLIARSGRSIDIVFTGLRPNEKLHEVLFSGDEERTTTSHELISRVAVPPLDP
                     EDLAGVDGSDPDSMSTLTLQRDAQAAARRAAADRG"
BASE COUNT          602 a          950 c         1038 g          563 t
ORIGIN      
        1 gccgcgagcg cgccaacgca ccagcgatag ggcatggctg ccccattggg caagtagaga
       61 gcaagagaag ccagggggat caacgcggcc cacaggacgt cagtgacgac ggccagcacg
      121 cgttgccggg tcgactcaca cactgtctag taccgcttcg aggcgggcag ctgccgccct
      181 gagggagaaa cggtcctcca tgatgcgtcg cccttcctgg ctccaacgga cgcgagtctg
      241 ggggtcggca tctcgaacgc gagcgatacc ttgggcgagt tcgtccggct cgacgaccgg
      301 acccgcctga tcatccttga ccacatcccg cagcgggatc ctcgcgttac tcacgatggg
      361 aattccggac gccatgtaat cgaacagctt attcgggctc atgcccttgt cgaatacgga
      421 ctggggggag atggtgtgga gtcccacgtc gcaagctttc agcagccacg gaagttccgg
      481 ctttccgatc gggtcacgga actcgacgtt ggtgaggcct cgattcgcag actcgctgat
      541 ggcccacgcc ttccgtgatc cattgccgac acacacaaag ttcacgtcag ggagcgccgc
      601 cgcggcgtcc agaatcaatt ccagtcctac gtagggtgaa tgattcccgg tgaagacggc
      661 cgtgaagccc gaaatgccgt agcgctgccg gagggcatct ttgctgtcgg gcacgtcata
      721 atcggccaag tctgcgccgt tgctgatgac ggtgaccttg ccgatatcga tgccgaggga
      781 ctcgaagtgg ttctcccagc ctttggtcac caccacgatc ttggctgccc gtcggtacag
      841 gagcctttcg agccaaacca aggccgtgtg cacgccggat ccggccctca aggtgccccc
      901 cgtcacgatc gattccggcc acaagtcacg gacttccaag acgaacgggc gccgacggac
      961 ggccgcgacc accatggcgc cggcagcggc cagcggatgg ggggaggagc cgaagtacac
     1021 gtctgctcgg gtcctcagcg ccttgagcgt cgccttgatg gcgaacaaga tccagcccag
     1081 gatgcgtcga ctcccgttgc cggagtacga ggggatggaa agcagggtcc accgtggatc
     1141 cttaaccacc atgacctcgc cggtgtagtg gttgcgggac gtagcgatga tccggaactg
     1201 ccagttcttg agccggctga acatgtcgaa gttccgggtg aggccgccgt actcacgtgg
     1261 gaaggcgtaa tggttgatca ccaatacccg tcgtttcccc ggcatctcag cgaccttcct
     1321 ggacaagcgt ctgcgctacc agctctgctg ccttgccctc cccatacggc atagcctgct
     1381 tggccgtggg ccgtggccga gcggcggcct cgcggacaag cgccaaggtg ggatctagca
     1441 cgttccagcc gtcagcgagt gtctccaccc attcggtttc ggtcctgatc gtggtgcacg
     1501 gagtctggag aagaaatgcc tccttctgca agcctccgga gtctgtgacc acaccagccg
     1561 cacattgcac cgttctgagc atctgaggat aggcgaggga gtcggttggg cggatgctcc
     1621 cttgcgtcag tgggatgccg aaggcggacg ccttggctcg caggcgtgga tgggtgagga
     1681 ggacgactgg gacagacagg acggccaagg cctcgatgat cgccgaaagc cggtcggggt
     1741 cgtcggtgtt atcggcccgg tgaatggtgg caaccacata gtcacccaga actccttcgg
     1801 gaagggtggg gttctggttc gccactgcat cgcgggtcgc gaagcaaacg tcagtcatga
     1861 catcacccac gaggcgagcg cgagacgaaa gcccctcgtc cttgaggtgt tgcatcgcaa
     1921 cctgagtcgg ggcaaggagc aggtcggcag catgatcggt caagacacgg ttgtgctctt
     1981 ccggcatcct ccggttgaac gagcgcagcc cggcttcgag atgggccaga ggtaggtgga
     2041 gcttgacagc ggccaccgcg cccgcgagcg tggagttggt gtccccgtac acgagcaccc
     2101 agtcgggcgc gtattcggac agcacgctgt cgagggcgct caagatggcg ccggtctgct
     2161 caccatggct gccggagccc acacccaagt ggacatgggg cttgccgata cccagatcgg
     2221 aaaagaaaac gtcggagagc agcgggtcat agtgctgtcc ggtgtgcacg atgacgtggt
     2281 cgatgtcgga ccgctcgcga aatgcgcgag cgatcggacc gagcttcacg aactgcgggc
     2341 gggctccgac gatagagagg atgcgcaccc ggtcatccta ggggatcgaa tcagccgcga
     2401 tccgcagctg ctcgccgggc cgcggcctgg gcgtcgcgct gcaaggtcag ggtggacatg
     2461 gaatcggggt ctgatccgtc aacacctgcg aggtcctcgg ggtcgagggg cggaacggcg
     2521 acccgactga tgagttcgtg tgaggtggtc gtccgctcct cgtccccgga aaacagcacc
     2581 tcgtgcagct tttcgttggg tcgaaggccc gtgaagacga tgtcgatgct tcggccagag
     2641 cgtgcgatca gacctcgggc cacgtcgagg atcttcacgg gctcgcccat gtccagaacg
     2701 aggacatctc ccggatttcc aatcgcccca gcctgaatcg tcagctcaca tgcctccgga
     2761 attgtcatga agaatcgagt gatatcaggg tgggtgacgg taaggggacc gccagcgtcg
     2821 atctgggccg tgaaggtatg cagcatcgag ccgcgcgaac ccaggacgtt gccgaagcgg
     2881 acagacaaga acgtacggtt cgtcctcgtg gcgaagtagg cggtgagctc ttccgccagc
     2941 cgtttcgtct ttccgagcac gctggtggcg tcagcggcct tatcggtcga aatgttgacg
     3001 aagcgctcca ccccatagcg ctccgccgcg cgcagcacat tgagcgtgcc gagggtgttc
     3061 gttttccagc cctcgagggg atactgctca agcatgggga ggtgcttcag cgctgcggca
     3121 tggaacacga catcgggccg gtggcccgcg aag
//
LOCUS       DAOSPL010000414         3146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_30875_length_3146_cov_3.010029, whole genome shotgun sequence.
ACCESSION   DAOSPL010000414 DAOSPL010000000
VERSION     DAOSPL010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30875_length_3146_cov_3.010029"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PLQ54_08600"
     CDS             <1..441
                     /locus_tag="PLQ54_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOS93358.1"
                     /translation="RPDLANTSIVEEDILVIPAEAKHPDEAFEFLKFVASEPGMELLC
                     TGQGKNSPRVRTSPKFMAEHPNPYIRVFSDLAKSPNAVTTPAFSLWQEYSTEMTTAFD
                     DIWLLRASPAAALQKVQARMQAKLDREIRQWQRRGKSYIPDEVR"
     gene            444..1388
                     /locus_tag="PLQ54_08605"
     CDS             444..1388
                     /locus_tag="PLQ54_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010495466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOS93359.1"
                     /translation="MASPVAARGGAQSRRNLRNGLFFVSPWLIGLSVFMLYPIVASFY
                     YSLCEYSVLQPAEFIGLGNYQDLFSDELFLKSLSNTLVYAAISLPLGLIVSLGLALLL
                     NTGVPFMSWYRTVFFLPALVPQVAIAIVWLWIFNGKFGVLNQALQPMLDVVGRALPGD
                     PKLTAPGWLSDPLWSKPALVLMGAWGVGNSVVIYLAGLQDVPKQLYEAAELDGASWSQ
                     RIRHVTLPALSPVILFNFIMGIIGSLQFFAVPYVLSPAGSPSRSIYFLTMYLYDSAFT
                     HLRMGYASAMAWILFLIIFVLTLLALRLSERHVYYGGE"
     gene            1532..2251
                     /locus_tag="PLQ54_08610"
     CDS             1532..2251
                     /locus_tag="PLQ54_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOS93360.1"
                     /translation="MDSLRAAVENYSKAIMYQWDRAEDVVPPFARYALNTLYVVVLAV
                     AGTVISNALVAYGFARIPWRGRNVLFALTLATMMIPFPVLMVSVFAIFRSLGWVGSFK
                     PLWIPAWFGSAFNIFLLRQFFRTIPNELSDAARIDGCSEVGIFRHVVLPLSKPALAVV
                     ALFCFMYTWNDFIGPLIYLTDKKMFTLSLGLQFYQSQHGGTQWNMLMAASTIVVLPLI
                     VVFFLTQRTFIQGITLTGLKG"
     gene            2423..2818
                     /locus_tag="PLQ54_08615"
     CDS             2423..2818
                     /locus_tag="PLQ54_08615"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOS93361.1"
                     /translation="MRSDRLIHTPWAVLTTMAACLALGGCEDMTDVIFPSNSNAAPTV
                     DAGPDQVITLPATAPLDGTVTRDGGPVLAGWTYTWTKVSGPGMVSFADPNAVDTVVSF
                     SAPGTYVLQLTATDGRQSASDQVTITVQG"
BASE COUNT          482 a         1066 c          962 g          636 t
ORIGIN      
        1 cggcccgacc tggcgaacac gtccatcgtc gaggaggaca tcctcgtcat accagccgaa
       61 gccaagcacc cggacgaggc attcgagttc ctgaagttcg tcgcctcaga acccgggatg
      121 gagctgctgt gtactggcca ggggaagaac tcgccgcggg tgaggaccag ccccaagttc
      181 atggccgagc acccgaaccc gtacattcgg gtgttctcgg acctggcgaa gagcccgaac
      241 gcggtgacca ctccggcttt cagtctctgg caggagtatt cgacggagat gacgacggct
      301 ttcgatgata tctggctctt gcgcgcatcg ccggcggcgg cgctgcagaa ggtccaggcg
      361 cggatgcagg ccaaactcga ccgcgaaatc cgccagtggc agcgccgcgg gaagtcctac
      421 attcccgacg aggtgcgcta gtcgtggcct ccccggtggc cgcccgtggc ggggctcagt
      481 cgcgccgcaa tctgcgcaac ggcctcttct tcgtcagccc ctggctcatc ggcttgtcgg
      541 tcttcatgct ctacccgatc gtcgcctcgt tctactatag cctgtgcgag tactcggtgc
      601 tccagcccgc cgagttcatc gggctcggga actaccagga cctcttcagt gacgagctgt
      661 tcctcaaatc cctctcgaac acactggtct atgccgccat ctcactgccg cttggcttga
      721 tcgtctcgct cggactggcc ctgctgctga acaccggagt gcccttcatg tcgtggtacc
      781 ggacggtgtt cttcctcccc gctctggtgc cccaggtggc catcgccatc gtgtggctgt
      841 ggatcttcaa cggcaagttt ggcgtgctca accaggccct gcagcccatg ctcgacgtgg
      901 tcgggcgggc tctgccgggc gatccgaagc ttacggcgcc cggctggctc tccgatccac
      961 tgtggtcgaa gcccgctctg gtgctcatgg gcgcgtgggg agtggggaac tccgtcgtca
     1021 tctacctggc tggtctgcag gacgtgccca agcagctgta cgaggccgcc gaactcgatg
     1081 gggcgagttg gtctcagcgc atccgccacg tcaccctgcc ggccctctcg cccgtcatcc
     1141 tgttcaactt catcatgggc atcatcggct cgctgcagtt cttcgccgtg ccctacgtgc
     1201 tttccccggc cgggtcgccc tcgcggtcca tctacttcct gacgatgtac ctgtacgaca
     1261 gtgctttcac ccacctgcgc atgggctatg cctcagccat ggcctggatc ctgttcctca
     1321 tcatcttcgt actcacgctg ctggccctcc ggctcagcga gcgacacgtc tactatgggg
     1381 gcgagtgacg ggtggacgta cggtccgcgt gggggaagct ggtggtcctg ctcatcctga
     1441 cggcgctggg tctcacattc ctggccccgc tgctctggat gatctccacc tcgctgaagc
     1501 ccatcgagga gacgatgacc ctcccgcccc agtggattcc ctcagagctg cggtggagaa
     1561 ctactccaag gccatcatgt accagtggga ccgggccgag gatgtcgtgc cgccctttgc
     1621 ccgctatgcg ctgaacacgc tctacgtggt tgttctggcg gtcgcgggga cggtcatctc
     1681 gaacgccctg gtcgcctacg gcttcgcgcg catcccgtgg cgcgggcgca acgtgctgtt
     1741 cgccctgact ctggcaacga tgatgatccc cttccccgtg ctgatggtct ccgtcttcgc
     1801 catcttccgc agcttggggt gggtcggcag cttcaagccc ctctggatcc ccgcgtggtt
     1861 cgggagcgca ttcaacatct tcctgttgcg tcagttcttc cgcaccatcc cgaacgagct
     1921 ctccgatgcg gcccgcatcg acggctgttc cgaggtcggc atcttccgtc atgtggtcct
     1981 gccgttgtcg aagccggctc tggccgtcgt cgcgctgttc tgcttcatgt acacctggaa
     2041 tgacttcatc ggtccgctca tctatctgac cgacaagaag atgttcaccc tctcactggg
     2101 cctgcagttc taccagagcc agcatggcgg cacgcagtgg aacatgctca tggccgcctc
     2161 gactatcgtc gtcctgcccc tgatcgtggt cttcttcctg acccagcgca cgttcatcca
     2221 gggcataacg ctgaccggcc tgaagggcta ggtcgctcta gcgcacgcac gggcccatgc
     2281 caataccgcg tggcggcgcg gtgcagttac ggctttcagg gaacggagga ggtcgcagcc
     2341 gttgtggacg aaaatgccta cggcgatacc tcagtcaccc ggtgggtgaa cgtcgacgcc
     2401 gctcatcgta ggagggatcg tcatgagaag tgaccgtctc attcatactc cttgggccgt
     2461 cctgaccacc atggcggcat gcctggctct cgggggctgt gaggacatga ctgatgtcat
     2521 ctttccctcg aactcgaatg ccgcgcccac ggtggatgcc ggccccgatc aggtcatcac
     2581 gctacccgct acggcccccc tcgacggtac cgtcactaga gacggcggcc cagtgctcgc
     2641 aggatggacg tacacctgga ccaaggtcag tggacccgga atggtgagtt ttgctgatcc
     2701 aaacgccgtc gacaccgtcg tatcgttctc agcgccgggc acctatgtgt tgcagctcac
     2761 ggcgaccgac ggacggcagt cggcctccga ccaggtgacc atcactgtcc agggctagtg
     2821 gccgggccct gcgtgggacc gacccctcgc cacatcgagc gcgggtcgcc gctagggccg
     2881 gtgcggcgag ggcgtgtcgc gcccgggcgg ttcggtacat gcgcttacgg acggcatggg
     2941 ggccgacggc ggtcaccggc tgcaggctgc cgcccgacct gtgggttgag tgcgcctccc
     3001 ccgactgaga ctacgccagg aactccacca ggtccgcgat gtccatctcg tgcgccgcgc
     3061 ccggcgtgac gtgcatcgag acggcatcgc ggggcgcgcc ggccgcggcg tatatggccc
     3121 ggagctcggc cagcgccggc tccacc
//
LOCUS       DAOSPL010000415         3144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_30902_length_3144_cov_3.645516, whole genome shotgun sequence.
ACCESSION   DAOSPL010000415 DAOSPL010000000
VERSION     DAOSPL010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30902_length_3144_cov_3.645516"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PLQ54_08620"
     CDS             complement(<1..588)
                     /locus_tag="PLQ54_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOS93362.1"
                     /translation="MTEMRRLARLAMVAALGAISASTIYPLVFMLLSALKSKREYLLN
                     RYGLPHEPTLVHFANAWTYGDMGQYLANSAIVVSAGVLLSWVVCGLAAYALSHLRFPG
                     RRAVFLAILGTMVIAPQVIIIPLYVTLIRCHLHDQHSGLILVYVTFATPFGTYLLTAY
                     YRAVPAELVEAAQVDGASHAQILWRIMVPIGRPAFL"
     gene            complement(581..1456)
                     /locus_tag="PLQ54_08625"
     CDS             complement(581..1456)
                     /locus_tag="PLQ54_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOS93363.1"
                     /translation="MRAQRRSAMVFAFLFLAPAAALLLTFRVVPAAGALWHSFTEWDG
                     VAPATYVGGANFARLVGDNTFWRAVLNNLVLVLCVPMWLGVSLVLALLIHQQVPGWRF
                     FRATFFLPSVLSPVIIGTLFSAILRRDGPVNRGLETAGLGALAHDWLAEPATALPTLV
                     MVILWGVFGMGVIVFLAGLSAVPSEILEAARLDGARGWTFVRYIIVPSLRHVIEFWAV
                     NLVVWSFTSLFAFVYVMTGGGPGYATMLVEYQLYLEAFQFNHMGYACALGSALFLMVL
                     GVVLIQIRLMTGGDD"
     gene            complement(1461..2792)
                     /locus_tag="PLQ54_08630"
     CDS             complement(1461..2792)
                     /locus_tag="PLQ54_08630"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOS93364.1"
                     /translation="MRGVILAATVVFGLALTSGCRKAGPPPAGERPLPSGPRAGAGGS
                     LTVWDWLSADPSQGVGHWLIDVDKAFEKAHPGVRVNHVAQSHTEYYQILKAAEAAAGE
                     AGGPDVVMIHQGSRVLDHKASLVPLDRYLKPELRKAIVGWELTSEGLSADGTPYAVPV
                     AVQGNAWYYNKSILTKAGVDPEAPPATWDEFLAVCDKVKASGVAPIACGEKEGDWADW
                     FLNSSSFLELSADEAAKLQRGEMAWTDPKVAALFTRLEQLGKRGMFQEGFLSTPLFPD
                     AGEVFMRGEAAFCLGLLSDVAHWKEFGEILGEGNLGVMPCPGTGMIPVGGGFAYGITS
                     WSQQPDLAFEYITLIVSDANANEFLKQAGSFPANQNFDPRLIADPNAKIIASWLAEKR
                     VAAPLTGKIPTEVVESIKREGQRILSGQTDVPGALKAIEATAARTKAGRAK"
     gene            complement(2804..>3144)
                     /locus_tag="PLQ54_08635"
     CDS             complement(2804..>3144)
                     /locus_tag="PLQ54_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93365.1"
                     /translation="AWMPGRIPDWAVQWHPQQASLFGQRWHVYVVARCDPAADQGSAF
                     QIGLYDGPNRIGLAQQTVSLADAGDGAYHTYDLGVHELQPGRYVWVAPPNSTVVNAVY
                     VDRVFVVREP"
BASE COUNT          578 a         1059 c         1046 g          461 t
ORIGIN      
        1 caggaacgct ggacgcccga tgggcaccat gattcgccac aggatctgag cgtggctcgc
       61 accgtcaacc tgggcggcct cgaccagctc ggccggcacg gcccgatagt aggccgtcag
      121 taggtatgtg ccgaatggcg tcgcaaaggt cacgtagacg aggatcagcc ccgagtgctg
      181 gtcgtgcagg tggcagcgga tcagtgtcac gtacagtggg atgatgatca cctggggcgc
      241 gatcaccatc gtgccgagga tggccaggaa cacggctctg cggcccggga agcgtaggtg
      301 cgagagggca taggccgcca ggccgcacac aacccacgag agcagcacac ccgcagacac
      361 gacgatggcc gagttggcca ggtactgccc catgtcgcca tacgtccatg cgttcgcgaa
      421 gtggacgagc gttggctcat gcggtaggcc gtaacggttg agcagatact cccgcttgct
      481 cttcagcgcc gatagcagca tgaagaccag cggatagatg gtcgatgcgc tgatggcgcc
      541 gagagcggcg accatcgcca ggcgggccag ccgccgcatc tcagtcatcg cctccggtca
      601 tgaggcggat ctggatcagc accacgccca ggaccatcag gaacagcgcg ctccctaacg
      661 cgcaggcgta gcccatgtgg ttgaactgga atgcttcgag atagagctgg tactcaacca
      721 gcatggtggc gtagccgggc ccgccgcccg tcatgacgta gacgaacgca aacaggctgg
      781 tgaaggacca caccacgagg ttcaccgccc agaactcgat cacatgtcgt aggctcggga
      841 cgatgatgta ccgcacgaag gtccacccgc gtgcaccatc gagccgggcg gcctcgagga
      901 tctcgctcgg caccgccgac aggccggcca ggaacacgat gacacccatc ccgaagacgc
      961 cccagaggat caccatcacc agggtgggca gggccgtggc gggctccgcc agccagtcgt
     1021 gcgccagagc gccgagacca gccgtctcga gcccgcggtt cacgggccca tcgcgccgga
     1081 ggatcgcgga gaacagcgtc ccgatgatca cgggagacag gaccgacggc aggaagaaag
     1141 tggcgcgaaa gaagcgccag ccgggcacct gctggtggat gagcagcgcg agcaccaacg
     1201 acacgcccag ccacataggg acgcagagga ccagcacgag gttgttcagc accgcgcgcc
     1261 agaaggtgtt gtcgcccaca agccgagcga agttcgcgcc gcccacatag gtcgcgggag
     1321 cgacaccatc ccactcggtg aacgagtgcc acagcgcccc ggcggcgggc acgacgcgga
     1381 atgtcagcag cagggccgcg gccggcgcca gaaacaggaa cgcgaaaacc atcgccgaac
     1441 ggcgttgcgc gcgcatgggg ctacttggcc cggccggctt tcgttcgcgc tgccgtcgcc
     1501 tcgatggcct tcagggcgcc gggaacgtcc gtctgaccac tcaggatgcg ctgcccctcg
     1561 cgcttgatgg actccaccac ctcggtgggg atcttacccg tgagcggcgc tgccacgcgc
     1621 ttctcggcca gccagctcgc gatgatcttg gcgttcgggt ccgcgatgag ccgcgggtcg
     1681 aagttctggt tcgcggggaa ggagcccgcc tgcttcagga actcattggc gttcgcgtcg
     1741 ctgacgatca gcgtgatgta ctcgaaggcc agatcgggct gctgcgacca cgacgtgatg
     1801 ccataggcga agccgccgcc caccggaatc atccctgtcc ccgggcaggg catcacgccg
     1861 aggttgccct cgcccaggat ctcgccgaac tctttccagt gcgcgacgtc ggataacaac
     1921 cccaggcaga aagcggcttc gccgcgcatg aacacctcgc cggcgtccgg gaagagtggg
     1981 gtcgacagga agccctcctg gaacatgccg cgtttcccaa gctgctcgag gcgggtgaac
     2041 agagcagcca ccttggggtc agtccacgcc atctcgccgc gctgcagctt cgcggcttca
     2101 tcagcgctca gttcgaggaa gctcgaggag ttgaggaacc agtcggccca gtcgccctcc
     2161 ttctccccgc aggcgatggg ggcgacgccc gaggccttca ccttatcgca gacggcgagg
     2221 aactcatccc acgtcgccgg tggcgcctcc gggtcgacgc ccgccttggt caggatgctc
     2281 ttgttgtagt accacgcgtt cccctggacg gcgacaggga cggcataggg cgtcccgtct
     2341 gccgagaggc cctcgctggt gagctcccac ccgacgatgg ctttcctcag ctcgggcttc
     2401 aggtagcggt ccagcggaac caggctggcc ttgtggtcga gtacgcggct gccctggtgg
     2461 atcatcacca cgtcgggccc gccggcctcc cccgcggcgg cctcggccgc cttgaggatc
     2521 tggtaatact ccgtgtgtga ctgggcgaca tggttgaccc tcacgcccgg gtgcgctttc
     2581 tcgaaggcct tgtcgacatc gatgagccag tggccgacgc cttgcgaggg atcggccgac
     2641 agccagtccc acaccgtgag cgacccgccg gccccggcgc gagggccgga cgggaggggc
     2701 ctctccccgg caggaggcgg gccagccttg cggcatccgc tggtgagtgc caggccgaag
     2761 accaccgtgg ccgccaggat cacaccgcgc atcggacgcc ctcctacggc tcacgcacca
     2821 cgaacacgcg gtcgacgtag accgcgttca ccacggtgct gttcggcgga gccacccaca
     2881 cataccggcc aggctggagc tcgtgaacgc ccaggtcata ggtgtgatac gcgccgtcac
     2941 cggcgtcggc aagcgacacc gtctgctggg cgagcccgat acggttcggg ccgtcgtaca
     3001 ggccgatctg gaatgcgctg ccctggtcgg cggccggatc gcatcgtgcg acgacgtaca
     3061 cgtgccaccg ctgaccgaac aggctcgcct gctgcgggtg ccactggacg gcccaatcgg
     3121 ggatgcggcc cggcatccag gcgg
//
LOCUS       DAOSPL010000416         3144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_30905_length_3144_cov_3.395274, whole genome shotgun sequence.
ACCESSION   DAOSPL010000416 DAOSPL010000000
VERSION     DAOSPL010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30905_length_3144_cov_3.395274"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1910
                     /locus_tag="PLQ54_08640"
     CDS             <1..1910
                     /locus_tag="PLQ54_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93366.1"
                     /translation="YLRLLETARRLFVPNPETQNLPMLYMPAWNGFVEGPTWGAWWIQ
                     NSYGTTYCALPFYEEPYVTFLQNSHDLWFDQMGDGQRAGANGWVAPDGCLCDAAAPGW
                     IVYRQGDGRTDIHDWGMEFTAAGLLMQSELLLISRDAAAIDHYLPMLERCAHFIESRR
                     DPANDLFLAGPAGNLLAPSYAGWARPDGTYDQAYLTGLSVTYIAALDRLIELERLARR
                     SFVADVLEGRRESARRGLSRLTTDEGYLIRSLDPDGTRHGVFGAQKHGYFEASPNHDA
                     ICFRVVDDIQAKRIYDKIASIPQLRPHAFILPNYPSYDDMYTAPEGLWGFGTWVNGGH
                     WSTCEGRAVMAYCRLGQFEDVRRSMNQLLTFVRRFRMDNPLVKMGSDVYQPNEPINLC
                     YDTFAPAAAMIRGLFEYLYKANGLTLIPHIPPGITELEQLDPIRFGAKRLYLATRGNG
                     PITGVRVNGAPWTRFSASQVDLPYDSVPGTAHVVVLMGGAAYRPSTLPWRSPEPPALE
                     ADGSVPEELEALVARAQKLRAFSQRLFGALLGDSYEAAHARLAVECVATALERARLRA
                     AGKIELLPEPSQTAADQSYIVTAAKLCDGLEAVLRTYEGSADPRKQQVLRLWQAEQGL
                     PSAHGPGTPSR"
     gene            2257..>3144
                     /locus_tag="PLQ54_08645"
     CDS             2257..>3144
                     /locus_tag="PLQ54_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOS93367.1"
                     /translation="MLHFDKRSRLFYVNLRPTRLEDVPEPNLYREIFPYSQFPKIPFS
                     NERVPTLIPEETWVTDTTFRDGQQSRPPYTVDQIVTLYKFLHRLGGPKGLIRQSEFFL
                     YSKRDREAVEKCLELGYEWPQITGWIRSVKSDFELVKQMGLKETGILMSCSDYHIFLK
                     LRRSRGEAMAMYLDLARAALDEGITPRCHFEDITRADFEGFVVPFAVELMKLAETYDA
                     PVKVRMCDTLGFGVPYVGADLPRSVAKITYLLHQRAGVPSEWLEWHGHNDFHKVLVNA
                     TTAWLHGCCASNGTLLSIGE"
BASE COUNT          527 a         1060 c          984 g          573 t
ORIGIN      
        1 agtacctgcg gctcctcgag accgcccgga ggctctttgt acccaacccc gagacgcaga
       61 acctgcccat gctctacatg cccgcctgga atgggttcgt ggagggcccc acttggggcg
      121 cctggtggat ccagaatagc tacggcacga cctactgcgc gctgcccttc tacgaggaac
      181 cctatgtcac gttcctccag aactcgcacg acctgtggtt cgaccagatg ggtgacggcc
      241 agcgggccgg cgccaatggc tgggttgcgc ccgatggctg tctgtgcgat gcggccgcgc
      301 ccggctggat cgtctaccgc cagggcgatg gacgtacgga catccacgac tgggggatgg
      361 agttcacggc ggcgggcctg ctgatgcagt cagagctgtt gctcatcagc cgcgatgccg
      421 cggccatcga ccactaccta cccatgctcg agcgatgcgc ccacttcatc gaaagccggc
      481 gcgaccctgc gaacgacctc ttcctggctg gtccggcggg caacctgctg gctcccagct
      541 atgctggttg ggcccggccc gacggcacct atgaccaggc ctatctcacg ggcctttcgg
      601 tgacctacat cgccgcgctc gaccgtctga tcgaactcga gcgattggcc cggcggagct
      661 tcgtcgccga tgtgctcgag ggccgacgcg agtcggcccg gcgcgggctg tcccgactga
      721 cgaccgacga gggttacctc atccgatcgc tcgatcccga cggcactcgc cacggcgtgt
      781 tcggtgccca gaagcatggc tacttcgaag cctcgcccaa tcatgacgcc atctgcttcc
      841 gggtcgtgga tgatatccag gccaagcgca tctacgacaa gatcgcgtcg atcccccagc
      901 tacggcccca cgccttcatc ctgcccaact acccctcgta tgacgatatg tacacggcgc
      961 ccgaaggact gtgggggttc ggcacgtggg tcaacggcgg ccactggtcg acgtgcgagg
     1021 ggcgcgcggt catggcctac tgccggctcg ggcagtttga ggacgtccgg aggtcgatga
     1081 accagcttct caccttcgtc cggcggttcc gcatggacaa cccgctggtg aagatgggca
     1141 gcgatgtcta tcaacccaac gaacccatca acctctgcta cgacaccttc gccccggctg
     1201 ccgccatgat ccgcgggctg tttgagtacc tctacaaggc caatgggctc accctgattc
     1261 cgcacatccc gccgggcatc accgagctgg agcagctcga tcccatacgc ttcggggcca
     1321 agcggctcta cctggccacc cgcggcaacg ggccgatcac cggagtgcgc gtcaacggcg
     1381 cgccgtggac gcgcttcagc gcgagccagg tggacctgcc ctatgacagc gttcccggta
     1441 ccgcgcacgt cgtggtactc atggggggcg cggcctaccg gccctcgacg ctcccctggc
     1501 ggtcgccgga acccccggcc ctcgaggcgg acggctcggt cccggaggag ctggaggcac
     1561 tggtagcccg agcacagaag ctacgtgcct tcagtcagcg gttgttcggc gctcttctgg
     1621 gcgactcata tgaagcagcg cacgcgcggc tggcggtcga gtgtgtagcc acggctctcg
     1681 agcgtgcgcg gctccgggct gcggggaaga tcgaactcct ccccgagcca tcgcagacgg
     1741 ccgcggatca gtcctacatc gtaacggccg ccaagctgtg tgatggcctg gaggctgtgc
     1801 tgcgaaccta tgagggctcc gccgatcccc gcaagcagca ggtgctgagg ctgtggcagg
     1861 ctgagcaggg gctgccgtct gctcatggcc ctggcactcc gagtcggtga cgcggggatc
     1921 caggagccca ctgggctcgg tgaactccag ggtctgctta accctgccca tgcggcgtct
     1981 gccgcgggcg agcgccatct atgagctcga gggctgcgct gccgccctct tctgtgtgcc
     2041 tttccctgac tgggccatgc ctgtgccagg ccgactcctt tcgcgctctc aacgccgcgc
     2101 gagagaagtt atgaattgat gctgaatata gtctcgcggg cttggctgct gcccaaaccg
     2161 gctctcgcct tgacagccgc cccgacgcgc gggttaccct gagagagtgc ccaatccgcc
     2221 gggcgccgga tcgcacggcg ccgagaggag cccgcggtgc tgcatttcga caagcgctcg
     2281 cgcctgttct acgtcaacct gcgtcccaca cggctggagg atgtgcccga gcccaatctc
     2341 taccgtgaga tcttcccgta cagccagttc cccaaaatcc cattcagcaa cgagcgggtg
     2401 cccacactca tccccgagga gacctgggtc accgacacga ccttccgcga tggacagcag
     2461 tcgcgtccgc cgtacacggt cgaccagatc gtcacgctct acaagttcct ccaccgcctg
     2521 ggaggcccga aagggctgat tcggcagtcg gagttcttcc tctactcgaa acgtgatcgc
     2581 gaggcagtcg agaagtgcct cgaactgggg tacgagtggc cgcagatcac cggctggatc
     2641 cgctccgtca agagcgactt cgagctggtg aaacagatgg ggctcaagga gaccggcatt
     2701 ctcatgtcgt gctccgacta ccacatcttc ctgaagcttc ggcgaagccg cggtgaggcc
     2761 atggccatgt acctggatct cgctcgcgcc gctctggacg aggggatcac gcctcgctgc
     2821 catttcgagg acatcacccg ggccgacttc gagggcttcg tggtgccctt cgccgtcgaa
     2881 ctcatgaagc tggccgaaac gtacgacgct cctgtcaagg ttcgtatgtg tgacactctg
     2941 ggcttcggtg tgccctatgt aggtgccgac ttgccgcggt ctgtggccaa gatcacctat
     3001 ctgctgcacc agcgggccgg tgtgccgtcg gagtggctcg agtggcatgg gcacaatgac
     3061 tttcacaagg tgctcgtcaa cgcgaccacg gcctggctgc atgggtgctg cgcctcgaac
     3121 ggcacactgc tttcgattgg ggag
//
LOCUS       DAOSPL010000417         3140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_30959_length_3140_cov_6.234360, whole genome shotgun sequence.
ACCESSION   DAOSPL010000417 DAOSPL010000000
VERSION     DAOSPL010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30959_length_3140_cov_6.234360"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..>1201)
                     /locus_tag="PLQ54_08650"
     CDS             complement(165..>1201)
                     /locus_tag="PLQ54_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93368.1"
                     /translation="EATMRYSRHLRTMTLCAVALLLLGGLSRAQAPGLAGAEWELRAL
                     GVTTPERLEALRADANKRPVTLAIVGQGGVSKSLLEAKLIEGNTLEYRQWPEGQDPDP
                     RASTHDTQAAHVILDLTLKLGLKVRVLVYQPGESTQSVAQAFAAAGAEADIVALYQSF
                     WGDVLPMAEAIGQAERALFISPYVEVGEPATNTCLQSYAAKPWADGYDHFVTVIPLAR
                     SQPDALISPLNRPELDTEVVNFIAPSFYASSAGGTCPSAEVAAAVAAWVVAASPEKPS
                     PEAIVELMRQGSVVDRESLTSLPEFTEEAVTAVERHISALSNPPAGERRRLDAAGVLN
                     LWAVREALGR"
     assembly_gap    1202..1301
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1711..2208
                     /locus_tag="PLQ54_08655"
     CDS             1711..2208
                     /locus_tag="PLQ54_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93369.1"
                     /translation="MRYWAVAASVLGLSSAMGGVQEPTPATDAPLFTIVYSADIDGYL
                     EPCGCGGINEGGIARQATLLKQLRAQGPVLAISGGGLGGIPEAVSAMVNALATMRYDY
                     LIPSAADLEICPAVLDEAASLGIRALRRAAPSSAVAPEVVEVAGLRVAIAGVSLPPPN
                     SRGLL"
     gene            2244..3023
                     /locus_tag="PLQ54_08660"
     CDS             2244..3023
                     /locus_tag="PLQ54_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOS93370.1"
                     /translation="MADRASRARLEELARVLGTLPVDSVLLPAAGAAGPAPVTARERC
                     LLPSLVARGRRLIALRCWRGPGDTGLRVAVSDEVVSADLAPDEELRALATDYYQKRQQ
                     TVLPTLAPLGAEGPGGATDLPLVLRVSEGTAYVPALGCGLCHPAQLERWRNTAHARAV
                     QALDEAKRELPECLRCHSTLFAFAGELEAPPDAPGGVECASCHGDGRRHLATRLPKDV
                     VAPVPANRCARCHTAEESPRFSDEYRSRLTCAESAVEEVEP"
BASE COUNT          419 a         1066 c         1073 g          482 t
ORIGIN      
        1 cccctattcg gcccctccga acacctcgcg ctggcagagg aggggccgaa tggaaggggc
       61 ccgcgtagcg ggaagggcgc catccggccc gctgtcggag tcgttctgag cgcagcgaat
      121 gatcttggcc cggggtcggt ccttgccacc ccccgccctg cgccctaccg ccccagcgcc
      181 tcccgcaccg cccacaggtt gagcacgccc gcggcatcga gccggcggcg ctcgcccgcg
      241 ggtgggttcg agagggcgct gatgtgccgc tcgacggccg tgacggcttc ctcggtgaac
      301 tcgggcaggg aggtcagcga ctcgcggtcg accaccgatc cctggcgcat gagctccacg
      361 atggcctcgg gcgagggctt ctccggactt gcggccacaa cccacgccgc cacggccgcg
      421 gcgacctcgg ccgaggggca tgtcccgccc gcagagctgg cgtagaaaga tggcgcgatg
      481 aagttcacca cttcggtatc caactcgggc cggttgagcg gcgagatcag ggcatccggc
      541 tggctccgcg ccagggggat caccgtgacg aagtggtcgt acccgtccgc ccagggcttg
      601 gctgcgtagg actggagaca cgtgttcgtc gctggctcgc ccacttcgac atagggcgag
      661 atgaacagcg cccgctccgc ctggccgatg gcctcggcca tgggcagcac atcaccccag
      721 aacgactggt agagggccac gatgtcggcc tcggccccgg ccgcggcgaa ggcctgcgcc
      781 acgctctggg tcgactcgcc gggctggtag acgaggaccc gcaccttgag cccgagcttg
      841 agggtcaggt cgaggatgac gtgcgcggcc tgcgtgtcgt gcgtgctggc gcgcgggtcg
      901 gggtcttgcc cttccggcca ctgccggtac tcgagcgtgt tgccctcgat gagcttcgcc
      961 tcgagcagcg acttgctgac cccgccctgc ccgacaatgg cgagggtaac cgggcgcttg
     1021 ttcgcgtcgg cacggagggc ttccagccgc tcgggcgtgg tgaccccgag tgcgcgcaac
     1081 tcccattcgg ctccggccaa gcccggcgcc tgggcgcggg agaggccgcc caggagcagt
     1141 agcgcgacgg cgcagagcgt catcgtgcgc aggtggcgcg agtacctcat cgtggcctcc
     1201 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncaccgacag agccgaactg
     1321 ggcattacca tcgtagtagc cccagttcga gccgactctg tctcgctccc cgtaccttgc
     1381 gaggcggttc ggagccaccg gagcgttcac ctactcggca cgaggtatcc accaacggcc
     1441 gccggggctg cgcgctggag ccgtgcccgc cctgaggctc gcaggctggt cgttggcaac
     1501 gtgtacctaa gaatcctcat cggtcttgac aggcatgcga ggcagccagt agattacgta
     1561 tgctcaactc acgcaaccgc gcccctcccg ggttggggat gctgagttga ccgaagtggg
     1621 tgccagggcg tggcgtgttg aggagtgcct ggggccggaa ccgtcccatc gcttggcgat
     1681 gagccggtag tctcgtccgg ggggcttgcg atgcgctact gggctgtggc cgcttcggta
     1741 ctcgggttga gctccgccat gggcggcgtt caggagccca cgcccgctac ggacgcccca
     1801 ctcttcacca tcgtctactc agccgacatc gatggctacc tcgagccgtg cggctgcggc
     1861 ggcatcaatg agggcgggat cgcccgccag gccacactcc tgaagcagct ccgcgcgcaa
     1921 ggcccggttc tggccatctc cggcggcgga ctcgggggca tccccgaggc tgtctccgcc
     1981 atggtcaacg ccctggcaac gatgcggtac gactacctga tccccagcgc ggccgatctg
     2041 gagatctgtc cggctgtcct tgacgaggcc gccagcctgg ggatcagagc gctccgacgg
     2101 gccgccccgt cctccgctgt tgcgcccgaa gtggtggaag tcgcggggct gcgcgtggcc
     2161 atcgccggtg tgtcgctccc ccccccgaac agccgtggcc tgctataggg ctgccggagg
     2221 ccggctgcga tctcctgctg gtcgtggctg accgggcgtc gcgagcccgg ctcgaggagc
     2281 ttgcgcgggt cctgggcacg ctgcccgtcg acagcgttct gctcccggcc gcaggtgcgg
     2341 ccggcccggc gccggtcact gcgcgggagc gatgcctgct cccgtctctc gtagcacgtg
     2401 ggcggcggct cattgcgttg cgctgctggc gcgggccggg agacacgggg ttgcgcgtgg
     2461 ccgtgtccga cgaggtcgtg tcggcggact tggccccgga cgaggagctg cgcgcgctcg
     2521 ccacagacta ctaccagaag cgtcagcaga ccgtgttgcc taccttggct ccgctcggag
     2581 ccgaggggcc gggcggcgcc acggacctgc cgctggtgct gcgcgtcagc gagggtaccg
     2641 cctatgtccc ggcgctgggc tgtggcctgt gccacccggc acagctcgag agatggcgga
     2701 acacggcgca tgctcgcgcc gtccaagcgc tcgacgaggc gaagcgcgag ctccccgagt
     2761 gcctgcggtg tcactccacg ctgttcgcct ttgccggcga gctcgaggcg ccgcccgatg
     2821 ctcccggtgg cgtcgagtgc gcctcgtgcc acggcgatgg ccggcgtcac ctggccacgc
     2881 gactccccaa ggacgtggtc gcgccggtgc cggcgaaccg gtgcgcccgg tgccatacgg
     2941 cagaggagag ccccaggttc tccgacgagt accggtccag actgacctgt gccgagtcgg
     3001 ccgtcgagga ggttgaacca tgacccaagc catgcttgcc gcgatcgtac tcctcgcagg
     3061 gagtgccgcc gcgacttcgc cgccgcacga gatcatggtg cccgtgccgc ccggcgaggt
     3121 gaccctccgc caggtctggg
//
LOCUS       DAOSPL010000418         3129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31129_length_3129_cov_3.729343, whole genome shotgun sequence.
ACCESSION   DAOSPL010000418 DAOSPL010000000
VERSION     DAOSPL010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31129_length_3129_cov_3.729343"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..1045)
                     /locus_tag="PLQ54_08665"
     CDS             complement(71..1045)
                     /locus_tag="PLQ54_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOS93371.1"
                     /translation="MARPATRQLLQGLGFCSPSIIGLAALMAYPMAASLYYSFCNYSV
                     LRPARFIGTANYRHLLTEDELFRTALYNTAVYAVWAVPLGIVFAFVLALLLNVKVRGM
                     AFYRTIFFIPSIVPLVASSVLWIWLLNPQIGGINFLLDRIGVDGLLTRFAETAPATAR
                     WLGFEGGRLPGWLSDPQWAKRGIILWSIWGVGGSVVIYLAGLQGVPKELYEAAEVDGA
                     GAWHRVRHITVPFMTPYLFFTLVMGLIGAFQFFTPAYVMTNGQGGPVDSTTFYSLYLF
                     NVAFADFKMGYASAMAWILFAIIVVGTVLVFKTSARFVYYGEGAGEGR"
     gene            complement(1149..2207)
                     /locus_tag="PLQ54_08670"
     CDS             complement(1149..2207)
                     /locus_tag="PLQ54_08670"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HOS93372.1"
                     /translation="MLALLLVPAVVLAAPPAGITKAVFENGDCVSGTLESVSGGQVRL
                     QTPYAGTVQLAIEQMVRLEVSQTVYVSPASGGDPIVADRMVLSDSKAVLVLADGTQRT
                     LALDAIAGIFVQPPQPPPPPEAPAAPPAPSKDEPEEEWAGNVVLGIGALTGNVNQVNW
                     RAGGQATRQSGRNQYTLNASWQRSDSEGAGSNTRVYGRGDYRRMGSDRLYYSGFVSGE
                     YDSAEQLEYRLEAGAALGYRLTASQWNRLGVELGLARTGERFQGTPGLGKWNLTTAID
                     GDRKFGSRTQLSGRLAHFANLASLSDYRVTADITVFRTLVKPYSLTVTLLDQYTNQPP
                     AGIRPNDLRLDIGLGYTF"
     gene            complement(2246..2899)
                     /locus_tag="PLQ54_08675"
     CDS             complement(2246..2899)
                     /locus_tag="PLQ54_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93373.1"
                     /translation="MPRPCRKCGMENEDQAQACAFCGARLGPGGLAGPGAATGPPRGG
                     MGLEETGRPGRQSSRALTVALWAIVIVLSCAVVFQVARRPRAVASQGPDDSSPLMGAK
                     AYMEALTGGDWAGLYQTLASPVQKVLARPALESRAAAAAQRGMACAMPQTGCAAIDQG
                     EIEVASGTIAYVAFECPTGTPQTVMLIDEAGHWKVCWSPLLDEVCGVPLPYIPPAAS"
BASE COUNT          535 a         1081 c         1021 g          492 t
ORIGIN      
        1 ctgaacacca ccagccccag gtgggccacc atgcggagcc acaggggctg ggttcggcca
       61 ccctcttcca tcagcgaccc tccccagcgc cttcgccgta gtagacgaac cgggccgatg
      121 tcttgaacac cagcacggtt cctacgacga tgatggcgaa cagaatccac gccatggccg
      181 aggcatagcc catcttgaag tcggcgaagg ccacgttgaa caggtagagc gagtaaaagg
      241 tggttgagtc caccggtccg ccctggccgt tcgtcatgac ataggccggg gtgaagaact
      301 ggaacgctcc aatgagcccc attaccaggg tgaagaacag gtagggtgtc atgaagggca
      361 cggtaatgtg gcggacccgg tgccaggctc cggcaccgtc cacttcggcg gcctcataga
      421 gctccttcgg cacgccctgc aagcctgcca ggtagatgac caccgatccg cccacgcccc
      481 agatgctcca caggatgatg ccccgcttgg cccattgcgg gtccgacagc cagccgggca
      541 gccggccgcc ctcgaatccg agccatctcg ccgtggccgg cgccgtctct gcgaaccgag
      601 tcagcagacc gtcgactccg atccggtcga gcaggaagtt gatgcctcca atctgcgggt
      661 tcagcagcca gatccacagc accgaactcg ccaccaacgg cacgatggat gggatgaaga
      721 atatcgtccg gtagaaggcc atgccgcgga ctttcacgtt cagcaacagg gcgagcacga
      781 aggcgaagac gatgcccagc ggaaccgccc agacggcgta caccgcggtg ttgtagaggg
      841 cggtccggaa cagctcgtct tccgtcagca gatggcggta gttggccgtg ccgatgaaac
      901 gcgccggccg gagcacgctg tagttgcaga agctgtagta cagcgacgcg gccatcgggt
      961 aggccatcag ggccgcgagg ccgatgatcg agggcgagca gaaccccagg ccctgcagga
     1021 gctgtcgggt ggccggacgt gccatggaga gtccttccgc tccgaacgtg gtgttccgcg
     1081 tctgtgccca cttcaagcag gggcccattg agacctgcgg gctgatcctc acgcgccgcg
     1141 tcagcgcgct agaacgtgta cccgagtccg atgtccagcc ggaggtcgtt gggccggatg
     1201 cccgctggcg gctggttggt gtactggtcg agcagcgtga cggtgagcga gtagggcttc
     1261 acgagcgtgc ggaacaccgt gatgtcggcc gtgacccggt agtcggatag actggccagg
     1321 ttggcgaaat gggccagacg cccggacaac tgcgttcgac tgccgaactt ccggtcacca
     1381 tcgatcgcgg tcgtcagatt ccatttgccg agcccggggg tgccctggaa acgttcgccg
     1441 gtccgggcca ggccgagctc cacccccaga cggttccact ggctggccgt gagccggtac
     1501 ccgagggccg cgccggcctc caggcggtac tcgagttgct cggccgagtc gtactcgccc
     1561 gagacaaacc cgctgtaata gaggcgatcc gagcccatcc ggcggtagtc ccccctgccg
     1621 tacacccgag tgttcgatcc cgcgccttcc gagtcgctcc gctgccacga tgcgttcaaa
     1681 gtgtactggt tccggccgct ctggcgggtc gcctgtcccc cggcgcgcca gttgacctgg
     1741 ttcacgttgc cggtcagggc gccgatgccc agcaccacgt tgcccgccca ctcctcctcc
     1801 ggctcgtcct tcgaaggggc cgggggtgcc gccggcgcct cgggtggcgg cgggggctgc
     1861 ggcggctgga cgaagatccc cgcgatcgcg tcgagcgcca gggttcgctg tgtgccatcg
     1921 gcgaggacga gtacggcctt gctgtcggac aggaccatcc gatcggccac gatgggatcg
     1981 cctccggaag ccggggagac gtacaccgtc tggctgacct caaggcggac catctgctcg
     2041 atggcgagct gcaccgtgcc cgcgtagggc gtctgaagcc ggacctgccc cccggagacc
     2101 gactcgaggg tcccggacac gcagtcgccg ttctcgaaga cggctttggt gatccccgcg
     2161 ggcggtgccg ccaggactac ggccggcacc agaagcaatg cgagcatggg tagagcaccc
     2221 tcacgcgacc ggagttcacc ggtgctcacg atgccgccgg cggtatgtag ggaagtggta
     2281 caccacagac ctcgtcgagg agcgggctcc agcagacctt ccagtgaccg gcttcatcga
     2341 tcagcatcac cgtctgcggc gttccggtgg ggcactcgaa ggccacgtag gcgatggtcc
     2401 cgctcgcgac ctctatctct ccctggtcga tggccgcgca acccgtctga ggcatcgcac
     2461 atgccattcc ccgctgtgcc gccgcagcgg ctcgcgactc cagggcagga cgggccaaaa
     2521 ccttctgcac ggggctcgcc agcgtctggt agagccccgc ccagtcgccg ccggtcaatg
     2581 cttccatgta ggccttggcg cccatcagcg ggctcgagtc gtccggcccc tgcgacgcga
     2641 ctgcgcgggg ccgacgggcc acctggaaca ccaccgcgca cgacagcacg atgacgatgg
     2701 cccacagtgc caccgtcagc gcccggctcg attgccgacc gggcctccca gtctcctcga
     2761 ggcccattcc tccgcgtgga ggccctgtcg cagcgcctgg cccggccagc ccaccgggcc
     2821 cgagccgcgc cccacagaag gcgcaggcct gggcctggtc ctcattctcc atcccgcact
     2881 tgcggcatgg tctgggcatt gccccagggt accctccgct cacttccggc gtcggccgcc
     2941 aaggtaccag acgccgggga gcaggtcaac gctggccgtc gcccgaagcc ctggggcacc
     3001 atggcgatcc gactgggatt ctgcaccgca ccatgccctg acattgagcc cggcgagctg
     3061 tgcgagctcc tacgccatgc cgggtaccgg gctatcgaac tgtgcgcgtg gaccgggcac
     3121 ccttgccaa
//
LOCUS       DAOSPL010000419         3128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31144_length_3128_cov_3.079401, whole genome shotgun sequence.
ACCESSION   DAOSPL010000419 DAOSPL010000000
VERSION     DAOSPL010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31144_length_3128_cov_3.079401"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..634
                     /locus_tag="PLQ54_08680"
     CDS             53..634
                     /locus_tag="PLQ54_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93374.1"
                     /translation="MPRQAIAYLITRCSEDRDSYLGAVLTTDDKSLPIEFFHTSPPVR
                     PTRLQRVLYGGALDAYIRRDVIGSPLLKALKERPALVISSDDLVLDLDGEAGCPVVRV
                     QQTNSEPLAHVGDTQELSPTQRLVQLRPKSGPIRVTGPRDDPALLSRVGELLAQLTET
                     MDILEPLDRVAQAVELIWEAEMNGKGVAESSTT"
     gene            583..1416
                     /locus_tag="PLQ54_08685"
     CDS             583..1416
                     /locus_tag="PLQ54_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93375.1"
                     /translation="MGSRDEREGRRGIVDNLIDTLRRALGLPSLVEIVPRIPEADVAG
                     VPQWVPGLVRPVDMRPAGGIHGGAATLETASAVPGDLDLSGQVVCTPPPSETVSVSGP
                     VPLELTAGVRQPEGTLEPVAVLALGDGLEVACIEPNGVRPSVPPPAVSAVRARPKTVS
                     VLRRPLRSACGVVAADEVAAALPVRRRALSHIRGHGAGLAAGMRALVRLSGLAPRDIR
                     WQGFYGPLPDAEPARMEVDADSGCLLIWYARGAAAKATLTLAVGRRSQDGTLIAARFP
                     Q"
     gene            1421..2029
                     /locus_tag="PLQ54_08690"
     CDS             1421..2029
                     /locus_tag="PLQ54_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93376.1"
                     /translation="MDRLLIAALLIANLAIIAYLCRLVIVVKRSLIHGTRDMDAEERV
                     HALHEALKVAVAEVDRVARESSRALDERQRELLDLLASCERVLREAQEVLDSLAPTRA
                     PGLAGSASAEPEAPPAQEPAALAEAPEAPPRELVARWRGFEKRHQRVLALSEEGLSME
                     AIAAEVDMSVGEVETVLCLERQFRGRDRGVAASAVSGSNLDS"
     gene            2161..>3128
                     /locus_tag="PLQ54_08695"
     CDS             2161..>3128
                     /locus_tag="PLQ54_08695"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOS93377.1"
                     /translation="MAVVALAAWVVGCGRQGSGSKPVAVVNGQNVTLNDVWRAVPMSQ
                     KSAALSFAVERVLWEGEAKKAGIEVTDADIEKEIQLQFGSAEDMKQYAEHNGMSADDL
                     KARLRLGVIMTRICTRNVKLTEDDIKAYFEKHRNEGNWDQPAQARLHVVYTKTKEQAN
                     DALRKLKTGADFDAVATEYALEGSPRGDIGWVMQPALQPRAAAEAIFDAKAKAGDLIG
                     PIPFAPPVQNETGTQVYCLFRIDELKPAKLASLDDPKVRAMVERQALISSPKAEKQEA
                     VAARLRSEAKVQILSPDFPQMQQQAGEGKTEGAAGTGASAGKNAAGG"
BASE COUNT          516 a         1009 c         1092 g          511 t
ORIGIN      
        1 cgggcctcgt gtgtggccct tcggcgccct ggggtgccga ggggcgtgac cgatgcctcg
       61 ccaagccatc gcctacctga tcacccgctg ctccgaagac cgtgactcgt acctgggggc
      121 tgtgctcacc accgacgaca agagtctgcc tatcgagttc tttcacacca gccctccggt
      181 gcgccccact cgcctgcagc gtgtcctgta cggtggcgct ctggacgcat atattcgccg
      241 cgacgtcatc ggctcgccct tactgaaagc gctcaaagag cggcctgctc ttgtcatcag
      301 ctccgacgac ctcgtgctcg acctcgatgg cgaggcgggt tgcccagtcg tgcgggtgca
      361 gcagaccaac tcagagcccc tcgcccacgt gggcgacacc caggagctta gcccaacgca
      421 gcgcctggtc cagcttcgcc ccaagagcgg gccgatccgc gtcaccggac cgcgcgacga
      481 tccggcgctc ctctcccgcg tcggcgagct gctggcgcag ctcacggaga ccatggacat
      541 cctggagccg ctggatcggg tcgcgcaggc agtggagctg atatgggaag ccgagatgaa
      601 cgggaagggc gtcgcggaat cgtcgacaac ctgatcgaca ctctccgccg cgcgctcggc
      661 ctgccctcac tggtggagat cgtcccccgg atacccgaag ccgacgtggc cggcgttccg
      721 cagtgggtgc ccggcctcgt acgcccggtc gacatgcggc ccgctggcgg catccatggc
      781 ggagcagcca cgttggagac cgcgagtgct gtgccaggtg acctcgatct ctccggccag
      841 gtggtgtgca cgcccccccc ctcggaaacc gtgtcagtgt cgggacccgt gcctctcgaa
      901 ctcacggccg gcgtgcgcca acccgaggga acgctggaac cagtggctgt acttgcactc
      961 ggcgatggcc tggaggttgc ctgcatcgag ccaaatggag tgcggccaag cgtgccgcct
     1021 cccgcagtgt cggccgtccg cgctcgcccc aagacggtgt cggtgctgcg caggccgctc
     1081 cgttcggcct gtggcgtcgt cgcagccgat gaggtcgctg ccgcgctccc ggttaggcgg
     1141 cgcgccctct cccacatccg aggtcatggt gccgggctgg ccgccgggat gcgggcactg
     1201 gtgaggctat ccggcctcgc cccgagggat atccggtggc aagggttcta cggcccgctg
     1261 cccgacgcgg agcccgcgcg aatggaagtg gacgccgact cgggctgttt gctaatatgg
     1321 tatgctcgtg gggctgcggc aaaggcgacg cttaccctcg cggtcggacg ccgctcgcag
     1381 gacggaactc tcattgccgc ccggttcccc cagtagggac atggatcgcc ttctcatcgc
     1441 ggctcttctg attgccaacc tggctatcat cgcgtatctg tgccgactgg tcatcgtggt
     1501 gaagcgcagc ctcatacacg gcaccaggga catggatgcc gaggagcgtg tccacgccct
     1561 gcatgaggca ctgaaagttg ccgtggctga ggtggaccgc gtcgcgcggg agtcttcacg
     1621 ggccctcgat gagcgacaga gagagctcct ggatctcctg gcctcgtgcg agcgtgtcct
     1681 gcgggaggcc caggaagttc tggattcctt agccccaacc cgagcccccg gcctggccgg
     1741 ttcggcatcg gccgagcctg aggcgccgcc ggcacaagag cctgccgctc tggcggaggc
     1801 tcccgaggcc ccgccccggg agctggtggc ccgttggcgc gggtttgaga agcgccatca
     1861 gcgcgtgctt gctctatcgg aagagggcct ttccatggag gccatcgcgg cggaagtgga
     1921 catgtctgtg ggagaggtcg agaccgtgct gtgtctggag cgccagttcc ggggccggga
     1981 caggggagtc gcggcatcgg cggtctccgg ctcgaacctg gactcgtagg gatggcagtg
     2041 tgggcgccac aggccgatgt cgtgcggtgg ccacggggcg ccctcgtggt gtgactcgga
     2101 agggtgttgg actcgcttat ggggcagttg cggtccgcgg cacgttgcgg gtggttggcg
     2161 gtggcagttg tggcgctggc ggcctgggtg gtggggtgcg gtcgccaggg ctccgggagt
     2221 aagcccgtgg cggtggtcaa cgggcagaac gtcaccctga acgacgtgtg gcgcgccgtc
     2281 ccgatgtctc agaagagtgc ggccctgagc ttcgccgtcg agcgagttct ctgggaaggc
     2341 gaggccaaga aggcgggtat tgaggtcacc gatgcggaca tcgagaagga gatccagctt
     2401 cagttcggat ccgccgagga catgaagcag tacgccgagc acaacggcat gagcgctgac
     2461 gacctcaagg ccagactgcg cctgggggtc atcatgacac ggatctgcac ccgcaatgtc
     2521 aagctcactg aggatgacat caaggcgtac ttcgagaagc acaggaacga aggtaactgg
     2581 gaccagccgg cccaggctcg actccatgtg gtctacacga agaccaagga acaggcgaac
     2641 gatgccctcc gcaagctcaa gaccggtgcc gacttcgatg cagtggccac cgagtatgcg
     2701 ctggaaggca gtccgagggg cgacatcggc tgggtgatgc agccggccct gcagcctcgg
     2761 gccgccgctg aggccatttt cgacgccaag gccaaagcgg gcgacctgat cggcccgatt
     2821 ccctttgcgc cacccgtgca gaacgagacc ggcacccagg tctactgcct gttccggatc
     2881 gacgagttga agccggccaa gctcgcatcg ctcgatgacc cgaaggtccg cgccatggtg
     2941 gagcgccagg cgctgatctc ctcgcccaag gcggagaagc aagaggccgt ggccgcccga
     3001 ctgcggagtg aggccaaggt gcagatcctg agccccgact tcccgcagat gcagcaacag
     3061 gcgggggaag gcaagacgga gggagctgca ggtaccgggg cgtcggccgg caagaacgcg
     3121 gccggggg
//
LOCUS       DAOSPL010000420         3127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31158_length_3127_cov_2.771159, whole genome shotgun sequence.
ACCESSION   DAOSPL010000420 DAOSPL010000000
VERSION     DAOSPL010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31158_length_3127_cov_2.771159"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLQ54_08700"
     CDS             <1..196
                     /locus_tag="PLQ54_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93378.1"
                     /translation="ELGLLNRDCPMIKLTMQDRHVIQTVALPGYPFVPMQLLVLPASF
                     ANVLAQASGDLSLRTGGRVA"
     gene            196..1212
                     /locus_tag="PLQ54_08705"
     CDS             196..1212
                     /locus_tag="PLQ54_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008360658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOS93379.1"
                     /translation="MRAVRLTTAQLDRAWGAVLGSAVGDALGAGYEFGSATVGSGGPA
                     MIGGGLGGFAPGEWTDDTSMAVAILEVAASGVDLRDESSLDRIASGFRAWFDTHPPDI
                     GVQTRRVLADAGPEPTAAKLTAAAYDLHVRMGRTAGNGGLMRTSPVALAHLDDDAATA
                     EAARRIGELTHFDPRSGEACTLWSLAIRHAILHAEFDIRSGLAQLDVESAAYWTERLD
                     EAEVGPASRFTNNEWVVEALQAAWSAIVHTPVPHGAGDGDAGMPCAHLPAALSTAIGI
                     GHDTDTVAAIAGALLGARWGASAVPAHWRRMLHGYPGINGERLVELATLAARGESRSA
                     TGGR"
     gene            2288..2848
                     /locus_tag="PLQ54_08710"
     CDS             2288..2848
                     /locus_tag="PLQ54_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93380.1"
                     /translation="MDEIIQAKQAVDLARARLEDAVHAARAQGRSWNEIGTLLGMSRQ
                     AAFKRFGRPADPRNGDPMTTRTITGLIEATEATFRLIADGSHDELQARLNPRVREQLT
                     PQLIGDTWVVALGMVGELEGFEGTHVELHDGTPLDADERVIGTVIGATVLRCEGGELV
                     GRVAFDETSAIVGILIVPTDHGPLPF"
     gene            2885..2989
                     /locus_tag="PLQ54_08715"
                     /pseudo
     CDS             2885..2989
                     /locus_tag="PLQ54_08715"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          475 a         1117 c         1050 g          485 t
ORIGIN      
        1 cgagctcggg ctcctcaacc gcgactgccc gatgatcaag ttgacgatgc aggaccgaca
       61 cgtgatccag accgtggcac tgcccggcta cccgttcgtg ccaatgcagc tcctggtgct
      121 gccggcgtcc ttcgcgaatg tgctcgccca ggcctcgggc gacctttcgc tgcgcaccgg
      181 tgggcgggtc gcctgatgag ggccgtcaga ctgacgacgg cgcagctcga tcgcgcctgg
      241 ggtgcggtgc tcggctcggc cgtcggtgac gcgcttgggg cggggtacga gttcggttct
      301 gccacggtgg gatcgggcgg gccggcgatg atcggcggcg gcctcggcgg cttcgccccc
      361 ggcgaatgga ccgacgacac gtccatggcg gtcgccatcc tcgaggtggc agcgtccggg
      421 gtggacctgc gcgacgagtc gtccctcgac cggatcgcgt cgggcttccg agcctggttc
      481 gacacccacc cgccggacat cggggtccag acccggcgcg tcctcgccga cgctggaccc
      541 gagcccaccg cagcgaagct gacggccgcg gcatacgacc tgcatgtgcg catgggccgc
      601 acggcgggca acggtggcct catgcgcacc tcgcccgtcg ctctggcaca cctcgacgac
      661 gacgcggcca ccgcagaggc cgcccgacgg atcggtgagt tgacgcactt cgacccacgc
      721 tccggagagg cttgcactct ctggtcgctg gccatccggc acgccatcct gcacgcggag
      781 ttcgacattc gctctggtct ggctcagctg gatgtcgagt cggccgccta ctggaccgaa
      841 cgtctcgacg aggcggaggt cggcccagcg tcgcgcttca ccaacaacga gtgggtcgtg
      901 gaagccctcc aggccgcctg gtcggcgatc gtccacactc ccgtccccca cggggccggc
      961 gatggcgacg ccggtatgcc gtgcgctcac ctcccagccg ccctctccac cgcgatcggc
     1021 atcggtcacg acaccgacac ggtcgccgcg atcgccgggg cgctgctcgg ggcgcgatgg
     1081 ggcgcctccg ccgttccggc gcactggcgg cggatgctgc acggctaccc gggaatcaac
     1141 ggcgaacggc tcgtcgagct cgcgaccctg gcagcccgcg gggaaagccg ctcggcaacg
     1201 ggtggccgct gaccgctcga gtcgactaca cggcatacac cggcaggaag gcgctggccc
     1261 gacagccgtt cgacgacgga gtctggctcg gcgacgtcga cgcgctcgag cagctgccgg
     1321 agggcgtctc cgccgtcatc tccctgtgtc tgctcggacg ccagcaggtg ccgccgggag
     1381 tcgagcacat cgcgttccgg ctcatcgacc gggctgaaac caggagaacc cccacctcga
     1441 gctcatcctg ggcgacgccg ccgcgaccgt caggaccttg cgtcgccgag gggcacgagg
     1501 ttcttgtccg ctgcgttgcg gccgagagcc ggacccccgc cgtggggatc gcctacgcgc
     1561 ttgccctcgg ggtccccttc gaggcttccc gcgacgccgt atgtgctgcg ctcccggcgg
     1621 ctcccccgaa cgccgccttc ctgcgcaccc tggccggacc cggggtcgaa agttacttcg
     1681 cagtccagga gtacatcggc gactgatcct caccaggtcg ccggcgacgg cgatctgagg
     1741 agcacatctt ggggccatcg tctgcacata gagcccgacg acgccatcgt cctcgaggcg
     1801 gaccgactcg cccgggcgca gcgcggccga gcatctcctc aggggcctct tcatacttcc
     1861 gatcaccgtg gaggcgggcc ggcgactggc gttggtcata tgccgcgcgc ggagtcacac
     1921 tccagcaggc ggactgcctc atcgcagcga ccccggctgc gcaccgggcc gtgctggcca
     1981 cggccaaccc gaaggacttc ccccacaggg aaaccgtggg tcgcgtgtct gtggcagggt
     2041 ggttctaggg tgttgttttt tccctttcca tgagaaacac ccacgacgtg cagcaggcga
     2101 gccagtggcg gtacgcggtg cccggctacg cgacgttcga cccggccccg atggggccgg
     2161 cggcataaat gtcgccccat agagccagcg caggatccac gccagtggcg cggcctcggt
     2221 ttctttgcgg caggggcatg tcaaccgatg gttgacacat gtgtcaacca tcggtttaca
     2281 tggagtcgtg gacgaaatca ttcaggcgaa acaggcggtc gacctcgcac gggcgaggct
     2341 cgaggacgcc gtccacgccg ctcgcgctca gggccgctcg tggaacgaga tcggcaccct
     2401 gctcggaatg tcacgtcagg ccgccttcaa gcggttcggg cgccccgccg acccacgaaa
     2461 cggagacccc atgaccactc gcacgatcac gggcctgata gaggcgaccg aggcaacgtt
     2521 ccgcctcatc gcggacggct cgcacgacga actccaggcc cgccttaacc cccgggtgcg
     2581 cgagcagttg acgccgcagt tgatcggcga cacctgggtc gtcgcgctcg gcatggtggg
     2641 cgagttggaa ggcttcgaag gcacccacgt cgagctgcac gacggcaccc cgctcgacgc
     2701 tgacgagcgc gtcatcggca ctgttatcgg cgccaccgtg ctgcgctgcg agggaggcga
     2761 gctggtcggc cgcgtcgcct tcgacgagac gagcgccatc gtcggcatcc tcattgtgcc
     2821 caccgaccac gggccgctgc cgttctgagc gaacggcacc accccggccg cgaagcggct
     2881 ccactcggcg cgacggggtg acttccgagt catctatcgg atcgatgacg agctagtcca
     2941 catccacgtc gtttccctcc ggcacagccg cgacgcctac cgcgcctgac cactgctcag
     3001 tggccgacgg gccagtgttg gacttccagc ccctgcatcg tgcttcgcgg ggcgacgtcc
     3061 tgcgggcagg tcggcgagca cgtcgtcggt ggtcaggaca tcacctgcag caagctcgtt
     3121 gagggag
//
LOCUS       DAOSPL010000421         3126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31168_length_3126_cov_3.413220, whole genome shotgun sequence.
ACCESSION   DAOSPL010000421 DAOSPL010000000
VERSION     DAOSPL010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31168_length_3126_cov_3.413220"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PLQ54_08720"
     CDS             complement(<1..232)
                     /locus_tag="PLQ54_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOS93381.1"
                     /translation="MRVLGIETSCDESAAAVAEDGVRLLSNVVASQVDLHARFGGVVP
                     ELASRAHLETINGCVEEALERAVAGLPTPAQVV"
     gene            complement(229..762)
                     /gene="rimI"
                     /locus_tag="PLQ54_08725"
     CDS             complement(229..762)
                     /gene="rimI"
                     /locus_tag="PLQ54_08725"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOS93382.1"
                     /translation="MTPEPIAIGPLTPAQLDDACEVERLCFATPWGREALEAELGRSS
                     MCCYVSATLPSGRTVGYAGMWVVGDEAHIGTLGVHPDYRRSGIGERLLVNLLLRAARR
                     GVGRVTLEVRASNVAAMSLYGKYRFRALGVRRGYYRDNGEDAVLMALEELDTERYQAF
                     IADRADALDCAPGGEDS"
     gene            complement(759..1475)
                     /gene="tsaB"
                     /locus_tag="PLQ54_08730"
     CDS             complement(759..1475)
                     /gene="tsaB"
                     /locus_tag="PLQ54_08730"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOS93383.1"
                     /translation="MMLAIETSGPVTSLVLWDGRVVGVESFESAQQLARRLVPAISGL
                     LSSRGLALSDLTKVAVGLGPGSFTGLRIGVTTVRALAHGLGLPLVGISSLRVLAEQAW
                     TGAGPPDGARVLAVARSKRGEVYAGCFGPGRELVEPFLLVQEEDLRALVSGLPRPLVL
                     AGRELLVRSAELGRDGGLIIPSSGDADYPDAAMLAALAWRQPAMSLASVTPIYVRRSQ
                     AEHARGEGPSPWLPGPVVSS"
     gene            complement(1472..1999)
                     /gene="tsaE"
                     /locus_tag="PLQ54_08735"
     CDS             complement(1472..1999)
                     /gene="tsaE"
                     /locus_tag="PLQ54_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOS93384.1"
                     /translation="MFRHSGGKQEGESSLWLPSPRDTLRLGQALGRCLEAGDCVALFG
                     PVGAGKTTLVQGMAEGTGSEAHATSPSFVLVHRYAGTPNLLHADLYRIERVSEFEDLA
                     LEEMAEAEGAAMAIEWCERCRGALPAVRLEVHMSYAAQGRQALPWAIGSRAEALLDAW
                     RRAGFGLRGATGDRP"
     gene            2317..>3126
                     /locus_tag="PLQ54_08740"
     CDS             2317..>3126
                     /locus_tag="PLQ54_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93385.1"
                     /translation="MPLLALAAVLVWTVPGSAQGAKKQLALAEVTNGSYYRGPDIADA
                     ARTALLTALMDSANTVVVEQAKVDEKIRELGLKSPLSDSELRQLGEALGCDVVVSGRV
                     LACSINDYSHTASARLRVGIYDAGAQASIRTTMVKGTGRSDNARATDRDLVAASLKDA
                     AYQAVQEIGQNLCIAATLLSAASEDRVLVSLPEGSGAKVGTELAVYRGTTEVARLIVY
                     KVTPGHSVCKLKTKTPLSQLQAGLRVLVVSTPSASLDEKDVKPKKKHHSSAL"
BASE COUNT          553 a         1156 c          980 g          437 t
ORIGIN      
        1 ccaccacctg ggccggcgtg ggcaggccgg ccacggcccg ctccagcgct tcctcaacac
       61 aaccattgat cgtctcgaga tgagcgcgcg aggccagctc gggcaccacg ccgccgaagc
      121 gcgcgtgcag gtccacctgg gacgccacca cgttggagag caggcgcacc ccgtcctcgg
      181 ccaccgcggc cgcagactcg tcgcacgagg tctcgatgcc caggaccctc acgagtcctc
      241 cccacctggc gcgcagtcca gggcgtcagc gcgatcggcg atgaaggcct ggtaccgctc
      301 ggtgtccagc tcctcgagcg ccatcagcac ggcgtcctcg ccattgtcgc ggtaatagcc
      361 ccgacggaca ccgagtgccc ggaagcggta cttgccgtag agcgacatag ccgcgacgtt
      421 ggaggcacga acctccaggg tgacgcgccc gactccgcgg cgcgctgccc gcagcagcag
      481 gttcaccagc agcctctcgc cgataccgga ccggcgatag tctgggtgaa cacccagggt
      541 gccgatgtgc gcctcatcgc cgaccaccca catgcccgca tagccgaccg tacggcccga
      601 cggcagcgtc gccgaaacgt agcagcacat cgagctgcgg cccagttcgg cctcgagcgc
      661 ctctcgcccc cacggcgtgg cgaagcacag tcgttcgacc tcgcacgcat cgtctagttg
      721 ggcgggcgtg agtggcccaa tcgcgatcgg ctcgggcgtc atgaagagac caccggcccc
      781 ggcagccacg gggaggggcc ctcaccccgg gcgtgctcgg cctgcgaccg ccgcacgtag
      841 atcggcgtta cgcttgccag actcatcgcc ggctggcgcc atgccagggc ggccagcatg
      901 gccgcatccg ggtagtccgc atcgccgctc gaagggatga tcagcccacc atccctacca
      961 agctcggccg aacgaaccag cagctcccgg cccgccagca ccagcggtct cgggagaccg
     1021 gagaccagcg ctctcaggtc ctcttcctgc accaacagga acggctcaac caactcgcgc
     1081 ccgggaccga aacaccccgc atagacctcg ccgcgcttcg atcgggccac tgcgagcact
     1141 cgggcaccat cgggcgggcc cgcgcccgtc cacgcctgtt cggcgaggac ccgcaagctg
     1201 ctgatgccaa ccaggggcag cccaagcccg tgcgccaaag ctcgaaccgt cgtcaccccg
     1261 atccgcagcc ccgtgaagga accaggcccc aacccaacgg ccaccttggt cagatcagac
     1321 agggcgagac cccgcgacga cagcagtccc gagatcgcgg gcacgaggcg ccgggccaac
     1381 tgctgggcgc tctcaaacga ctccacaccc acgacccggc catcccacaa gaccaggctg
     1441 gtcacagggc cggaggtctc gatggcgagc atcatgggcg atcacccgtg gcaccgcgaa
     1501 ggccaaagcc cgcacgcctc cacgcgtcga gaagtgcctc ggcccgcgac ccgatggccc
     1561 acggcagcgc ctgccgcccc tgggccgcgt aactcatgtg cacctcgagc cggacggctg
     1621 gaagcgcgcc acgacaccgc tcacaccact cgatggccat cgccgcgcct tcggcctccg
     1681 ccatctcctc caatgccagg tcctcgaact ccgacactcg ctcgatgcgg tagaggtccg
     1741 catggagcag gttcggcgtg cctgcatagc ggtgaacgag cacaaaggat gggctcgtgg
     1801 cgtgggcctc gctaccagtg ccttctgcca tgccctgcac caaagtggtc ttacctgccc
     1861 ccacgggtcc gaacaacgcc acgcagtcgc ccgcctcgag gcagcgcccc agagcttggc
     1921 ccaaccggag cgtatcgcgg ggggagggaa gccacaggga ggattccccc tcctgcttcc
     1981 cccccgaatg cctgaacatc ggcaagtcct cggggctttt ataggcggtc gcactggaag
     2041 gttaggcgcc gggtgtgggt gtgtcaagcg gcctgggacc gggtcgcggg acctccggcg
     2101 ccccatgctc catccctccg aagccactcc tggcagcttc gaggggcata tgccttggct
     2161 gcaggagtga gcggtccgct cccgggtctt cggggcccga gagacggcct caggtcgtgt
     2221 tcgccggcct ggtgtaggag gaatgcagcg atgccatcac gcgacgcagg ctccccatgc
     2281 gtgcggcgag ccagcgcgac gagagcggcc catctcgtgc ccctactcgc tctcgcggcc
     2341 gtgcttgtgt ggaccgtgcc cggctcggct cagggcgcca agaagcagct cgccctcgcc
     2401 gaagtgacca acgggtccta ctaccgcggt ccggatatcg ccgacgccgc gcgcaccgcg
     2461 ctgctcacgg ccctcatgga ctcggccaac accgtagtgg tcgagcaggc caaggtcgac
     2521 gagaagatcc gggagctggg cctcaagtcg cctctgtcgg attccgagct gcgccaactc
     2581 ggcgaggccc tgggatgcga cgtggtcgtc agcggacgcg tgttggcctg ctccataaac
     2641 gactactcac acacggccag cgcgcgactc cgcgtcggta tctatgacgc cggggcccaa
     2701 gcctcgattc gcaccaccat ggtgaagggg acgggccggt cggacaacgc ccgagccacc
     2761 gatcgtgacc tcgtggcggc gtcgctcaag gacgcggcct atcaggcggt ccaggagatc
     2821 ggacagaacc tctgcatcgc cgccacgttg ctctccgccg cctccgagga ccgcgtgttg
     2881 gtcagcctcc ctgaggggtc gggggccaag gtcggcaccg aactcgctgt gtaccggggc
     2941 accaccgaag tggctcggct catcgtctac aaggtcacac cgggtcattc ggtctgcaaa
     3001 ctcaagacca aaacgcctct ctctcagctc caggcgggcc tccgtgtgct cgtggtgtca
     3061 acaccatcgg cctcgctgga cgagaaagat gtgaagccca agaagaagca ccactcgagc
     3121 gcccta
//
LOCUS       DAOSPL010000422         3124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31193_length_3124_cov_3.069078, whole genome shotgun sequence.
ACCESSION   DAOSPL010000422 DAOSPL010000000
VERSION     DAOSPL010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31193_length_3124_cov_3.069078"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PLQ54_08745"
     CDS             <1..358
                     /locus_tag="PLQ54_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93386.1"
                     /translation="YFENEMRPDLTVVDVGARLEIEPWTGNREDITVHLSPAVSSIRG
                     EDPITGMPVLSVRRAEATVRVRAGEPIVLGGLSMFSDESTHHRLNLPVIQSIFRGRGR
                     TEQNAEFSFVVTPRVL"
     gene            369..2111
                     /locus_tag="PLQ54_08750"
     CDS             369..2111
                     /locus_tag="PLQ54_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93387.1"
                     /translation="MRRRGTWLSLWLLGLISAQAQAIVYCNITDVRCDVLSNGIQVTV
                     ASDGILEYQHPTEQWQPRYWSGKSLTLLFPGARSKFGRNFIDVSKFPVSFIQLSIPTT
                     AQEGVGILLTVSAMANTDFRISQAANRQSVIITVASTRTLEKGAGATATSGGEAQLTV
                     ETRNGTASVHAVNADVHEVFARVAQALNLDVAVDDGVDRRISVFMADVPGEDLLRAIA
                     RAAGLALVKTPDGTFMVSAGVVEDLATYRLTETRSFRMHNIQASSAAQLLPTFIFPYL
                     HQNKPQNAVVVTAQEDMLEKIEADLRAIDKAPPQIMIEVLAVEFIDTREAERSLALLH
                     NTAGSADTYDSTLGDISYSTIGVLPEEFLANVNWLKSKGKAVIKAQPKMAVLNGGQAR
                     IFIGSTRFIEVEVSQWGSVSRRIQGVNVGVTLDISPWTGESREITTTIAPTVSNISEL
                     DSETGLPVLSTRDAKTQVRALSGETIMIGGLSLEQAQVTSHKTKVLGDLPLIGKLFRR
                     KATQRTESLLVIFVTPRLLDERGRLPEAERESIHKRFLESDHGWQEPATIEVKRSGDR
                     LSWRETVATPASGS"
     gene            2132..>3124
                     /locus_tag="PLQ54_08755"
     CDS             2132..>3124
                     /locus_tag="PLQ54_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93388.1"
                     /translation="MTWRARTRGKPGRVCAGIAWLSLVCTCGCGLSAGGGEGAAGSDY
                     EVLYVRDGRLVAVHPDGSDARVVNSALTGCREPQGSPDGRFIACVSGAFVYIMARDGS
                     GAHAVAGRVATAEPVYPNWSPDGRKLCFAGLDQRVHVLELDATMGPERETAIAAGPGG
                     APATPSFLTNQRLLFAARAPEAAGPGQGDGWLYTVNIDGSGLLPFFSSATSVYGVDPT
                     RGNVRRAVRANRADWIVFESDVRGGYDLWLAYLDGDVYGCVTQYDYPVGESARGTAAW
                     ERNVAGRWTPDGRIAYESAHYDALGWGVPQIWITDKFGSYPVQLTHDPEGAHQPG"
BASE COUNT          529 a         1089 c         1008 g          498 t
ORIGIN      
        1 ctacttcgag aacgagatgc gaccggatct gacggtggtc gacgtcggcg cccggctcga
       61 gatcgagccg tggaccggca accgcgagga catcaccgtc cacctctccc ccgcggtctc
      121 gagcatccgc ggcgaggatc cgatcaccgg tatgcccgtg ctgagtgtgc gcagggccga
      181 ggccaccgtc cgcgtgcggg ccggcgagcc aatcgtgctg ggcgggctca gcatgttctc
      241 cgacgagagc acacaccacc ggctcaacct gccggtcatt cagtccatat tccggggtcg
      301 aggccgcacg gagcagaacg ccgagttcag cttcgtggtc acccccaggg tgctgtaggg
      361 gcggcgagat gagacgtaga ggaacatggc tgtcgttgtg gctgctcggc ctgatctctg
      421 cccaggctca agccatcgtc tactgcaaca tcaccgacgt ccggtgtgac gtgctctcca
      481 acggtatcca ggtcaccgtg gcatcggatg gcattctgga gtatcagcat cccaccgagc
      541 agtggcagcc gcgttactgg agcggcaaga gcctgacgct cctgttcccc ggcgctcgct
      601 ccaagttcgg acgcaacttc atcgacgtga gcaagttccc cgtgagcttc attcagctgt
      661 ccattcccac cacggcccag gagggcgttg gcattctcct gaccgtatcg gcgatggcca
      721 acacggactt ccgcatctcc caggccgcca accgccaaag cgtcatcatc acggtggcca
      781 gtacgcgcac gctcgagaag ggagccggtg cgacagccac ctcgggtggc gaggcccagc
      841 tcaccgtcga gacccggaac ggcaccgcgt ccgtccatgc cgtgaacgcc gacgtgcacg
      901 aggtgttcgc ccgagtggcg caggcgctca acctcgacgt cgccgtcgat gacggcgtcg
      961 accgtcgcat cagcgtcttc atggccgacg ttccgggtga ggacctgctg cgagccattg
     1021 cccgggccgc cgggctcgcc ctcgtcaaga cccccgatgg caccttcatg gtcagcgcgg
     1081 gtgtggtcga ggatctggcg acctatcgtc tcaccgagac gcgatccttc cggatgcaca
     1141 acatccaggc gtcctcggcc gcccagctgc tgcccacctt catcttcccc tacctgcacc
     1201 agaacaagcc ccagaacgcc gtggtcgtca ccgcccagga ggatatgctc gagaagatcg
     1261 aggcggacct gagggccatc gacaaggcgc cgccccagat catgatcgag gtcctggccg
     1321 tggagttcat cgacaccagg gaggccgagc ggtccctggc gctcctccac aacacggcgg
     1381 gctccgccga cacctacgac tcgaccctcg gcgatatcag ctactcgacc atcggcgtgc
     1441 tgcccgagga gttcctcgcc aatgtgaact ggctcaagag caagggcaag gcggtcatca
     1501 aagcgcagcc gaagatggct gtcctcaacg ggggtcaagc gaggattttc atcgggtcga
     1561 cccggttcat cgaggtcgag gtgagtcagt ggggcagtgt gtcccgtcgc atccagggag
     1621 tcaacgtggg cgtgacgctc gacatctcac cgtggaccgg cgagagccgg gagatcacga
     1681 ccaccatcgc gcccaccgtc tcgaacatct ccgagctcga ctcggaaacc ggcctccccg
     1741 tactcagcac gcgcgatgcc aagacgcagg tccgggcgct cagcggcgag accatcatga
     1801 tcggaggcct ctccctcgag caggcgcaag tcacgagcca caagacgaag gtccttggtg
     1861 atctgcctct catcggcaaa ctcttccgcc gcaaagcgac ccagcgaacc gagtccctgc
     1921 tggtcatctt cgtcacaccc aggctgctcg atgagcgcgg ccgactgccg gaggccgagc
     1981 gcgagtcgat ccacaagcgg ttcctggagt ccgaccacgg gtggcaggaa cccgcgacca
     2041 tcgaggtgaa gcgctcgggt gaccgcctga gctggaggga gaccgtcgcg actccggctt
     2101 cggggtcgtg atcgggaggc gagaggcccc gatgacctgg agggcgcgca cgcgaggaaa
     2161 acccgggcga gtttgtgcgg gcatcgcctg gctttccctg gtctgcacct gtggctgcgg
     2221 gctgtccgcg ggtgggggtg agggagccgc cggctcggac tacgaggtgc tctacgtccg
     2281 cgatggccgc ctcgtcgccg ttcaccccga tgggtccgac gcccgcgtgg tgaactctgc
     2341 actcaccggc tgccgcgagc cccaggggtc gcccgatggg cggttcatcg cctgcgtctc
     2401 cggtgccttc gtctacatca tggcccgcga cggctccggg gctcacgccg tcgctgggcg
     2461 cgtcgccacc gccgagccgg tctatcccaa ctggtcgccc gacgggcgga agctgtgttt
     2521 cgccgggctg gaccaacgcg tccacgtgct cgagctcgac gccaccatgg gcccggagcg
     2581 cgagacggcc atcgcggcag gcccgggcgg cgcgcccgcc acaccgagct tcctgaccaa
     2641 ccagagactg ctcttcgccg cgcgcgcccc cgaggcggcc gggcccggcc agggcgacgg
     2701 ctggctctac acggtgaaca tcgatggcag cggcctgctg cccttcttct cctccgccac
     2761 ctcggtctac ggcgtcgacc cgactcgcgg caacgtccgg cgcgccgtcc gcgcgaaccg
     2821 cgcggactgg atcgtgttcg agagcgatgt tcgcggcggc tacgacctgt ggctcgccta
     2881 cctcgatggc gatgtctacg gctgtgttac gcaatacgac tacccggttg gcgagtcggc
     2941 gcgaggcacc gcggcctggg agcgcaatgt ggccgggcgc tggacacccg acggccgaat
     3001 cgcctacgag tccgcccact acgatgccct gggatggggc gtgccgcaga tctggatcac
     3061 cgacaagttc ggctcatacc ccgtgcagct cacccacgac cccgagggtg cacaccagcc
     3121 aggg
//
LOCUS       DAOSPL010000423         3120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31256_length_3120_cov_3.205546, whole genome shotgun sequence.
ACCESSION   DAOSPL010000423 DAOSPL010000000
VERSION     DAOSPL010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31256_length_3120_cov_3.205546"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1575
                     /locus_tag="PLQ54_08760"
     CDS             <1..1575
                     /locus_tag="PLQ54_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93389.1"
                     /translation="MRIRRNGALALLGVAVALGSAPEGARADEGPQTGLWVYPAPGQD
                     EYRSEHYRVTVSLGGEAREAFCYRMRSIWHREFAGDWAAQFFPKDNNWVSFSFAGAVT
                     VVVDPLDVQAIEVEVRPDPPGVSASVREGKVVVEISRPGQYYVMVKAAENPQHDFDHP
                     LFLFANPPETDVPTLDTPGVHYFGPGVHDIGFQYRVTGGETVYIAGGAMVRGSLYYEG
                     DGVRILGRGILTDRQLMLERVNSALAEKAAGKANAWDLNHDAMYRDKWATIYGDVDAD
                     GALIEGITIIESPFYMVRTHGAGTVFRNLKLMSDLYNNNGIVAGPNHQILDCFFKVED
                     DVFCWMAPVSETRNCVIWKQDNSCMVQLGYGYAYATHGHVFADNWVIRDVTSVQVQAR
                     GLFGLAASIGTTFADCRIENLKVWGDVLNFLAIDNWDRPTPWAAKPDGEVRLRPVDLT
                     FRNVEITGTEKGDYWGPDMADLVGSPMRSRLRTEKEGSIHIVLENVRIHGKLLRSDAD
                     WPNGLLQQGQVTVEYR"
     gene            complement(1629..>3120)
                     /locus_tag="PLQ54_08765"
     CDS             complement(1629..>3120)
                     /locus_tag="PLQ54_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOS93390.1"
                     /translation="AAGETVIVTGADVLTGWARVGGDAPIYRVPWTHEFVIDMRDGTP
                     VRHHPGDEEHSRSGRAEQVIVDGEVWDYPQLVLSLAEMTPGTFFPDPDSGFLYIRMAD
                     DSDPAVHQIEGATRGLVFGANPWMQGASFDHVVVRGFTFRYGATFPQRPGVWLLGEAN
                     ALEDCTIEWMAGGGAGVGPKRGAMRRCIIRHCGHTGGCAGGEDFVNEGCTWEDNCRKP
                     ISRGWDAGGVKLAVSRHGLFDRCVFRDNGGPGLWFDIDVSDTVVRQCLFEGNEQQGLF
                     IEISRDSTVEDCAFFGNGLRSGEGWSTAGLTLAESQHCTIVHNLLVGNLDGLSLREQG
                     PRYLDTADLGPLAFQNVGHIVAGNISANNQRYQLGLWYDTAFFGMHPGDKGKYASEEE
                     FAAVIQRDQPDRWFDPLQQGMTFAHNAYFAGPGQKLFLYGVPWRARFREFDDVASLTA
                     ATGFEAGSVVADPGLRDLGEGRFELPRTGDLFAQGFGPRWKPAGMR"
BASE COUNT          552 a         1063 c          997 g          508 t
ORIGIN      
        1 atgcggatca gacgaaatgg agccctcgct ctcctgggag tcgccgtcgc cctggggagt
       61 gccccagagg gggcgcgcgc cgatgagggg cctcagactg gcctgtgggt gtaccccgcg
      121 ccggggcagg acgaataccg ctcggagcac taccgggtca ccgtgtcact cggcggggag
      181 gccagggagg cattctgcta ccgtatgagg tcaatctggc accgcgaatt cgcgggcgat
      241 tgggcggcgc agttcttccc gaaggacaac aactgggtga gcttctcctt tgccggcgcc
      301 gtgaccgtgg tggtcgatcc cctcgacgtg caggccatcg aagtcgaggt ccggcctgat
      361 ccgccaggcg tctccgcatc agtccgcgag ggcaaagtag tcgtcgagat cagcaggccc
      421 gggcagtact acgtgatggt gaaggccgcg gagaaccctc agcatgactt cgaccacccg
      481 ctcttcctgt tcgcgaaccc gccggagacc gacgtaccaa cgcttgacac tcccggtgtc
      541 cattacttcg gccccggcgt gcatgacatc ggcttccaat accgtgtgac tggtggcgag
      601 acggtctaca tcgccggcgg cgccatggtc cgcgggtcgc tctactacga gggcgacgga
      661 gtacggatac tgggccgcgg aatcctcacg gacaggcagc tcatgctcga gcgagtcaac
      721 tcggcactgg cggagaaggc ggccgggaaa gccaacgcct gggacctcaa ccacgacgcc
      781 atgtaccgcg acaagtgggc gacgatctat ggggatgtgg acgccgacgg ggcgctgatc
      841 gagggcatta cgatcatcga gtcgcccttc tacatggtgc ggacacacgg ggcgggcacg
      901 gtcttccgca acctcaagct catgtcggac ctgtacaaca acaacggtat cgtcgccgga
      961 ccaaaccacc agatccttga ctgcttcttc aaggtcgagg acgacgtctt ctgctggatg
     1021 gcgcccgtct cagagacgcg caactgcgtg atctggaagc aggacaactc ctgcatggtt
     1081 cagctcggct acggctacgc ctacgccacc cacggtcacg tgttcgccga caactgggtg
     1141 atccgcgacg tcacgagcgt ccaggtccag gcacgcgggc tgttcgggct tgcggcgtcc
     1201 atcgggacga ccttcgccga ctgccgtatc gagaacctga aggtgtgggg cgatgtcctc
     1261 aacttccttg ccatcgacaa ctgggatcgc ccgacgccct gggccgccaa gcccgacgga
     1321 gaggtgcggc tccgtccggt agacctcacc ttccgaaacg tggagatcac cgggacagag
     1381 aagggcgact actgggggcc ggacatggcc gacctggtgg gcagcccgat gcgctcccgt
     1441 ctacgtacag agaaggaggg ctccattcac atcgttctcg agaacgtgcg catccatggg
     1501 aagctcctcc gcagcgatgc cgactggccc aatggtcttc tccagcaagg gcaggtcacg
     1561 gtcgaatacc gatagcgtcg ccccgggcca tccgccgcga ggcccggcag gtccaggcca
     1621 gaccccgcct agcgcatgcc cgcaggcttc cagcgcggcc cgaacccctg cgcgaacagg
     1681 tcgcccgtcc gcggcagctc gaagcgcccc tcgccgaggt ctctgaggcc cggatccgct
     1741 accacgcttc ctgcctcgaa cccagtggcc gccgtcagtg aagccacgtc gtcgaactcc
     1801 cggaagcgtg cccgccatgg cacaccgtac aggaacagct tctggcccgg gccggcgaag
     1861 taggcgttgt gcgcgaaggt catcccctgc tgtagcgggt cgaaccaccg gtcgggctgg
     1921 tcgcgctgga tgaccgcggc gaactcctcc tcgctggcgt acttgccctt gtcacccggg
     1981 tgcatgccga agaaggccgt gtcataccaa agcccgagct ggtaccgctg gttgttggcg
     2041 ctgatgttgc cagcgacgat gtggcccacg ttctggaaag ccagtgggcc gaggtccgcc
     2101 gtgtcgaggt accttggccc ctgctcgcgg agcgagaggc catcgaggtt gcccaccaac
     2161 aggttgtgta cgatggtgca gtgctggctc tcggcgaggg tcagtcccgc cgtgctccat
     2221 ccctcgcccg accggaggcc gttgccgaag aacgcgcagt cctccaccgt gctgtcgcgg
     2281 ctgatctcga tgaacaaccc ctgctgctcg ttgccctcga acaggcactg gcgaaccacc
     2341 gtgtccgaca cgtcgatgtc gaaccacaag cccggcccgc cgttgtcgcg gaagacgcac
     2401 cgatcgaaca ggccgtggcg cgagacggcg agcttcacac cacccgcatc ccacccacgc
     2461 gagatgggct tccggcagtt gtcttcccag gtgcagccct cgttgacgaa gtcctccccg
     2521 cccgcgcaac cgcccgtgtg accgcagtgg cggatgatgc accgccgcat ggcacctcgc
     2581 ttcggcccca cccccgcgcc gcccccggcc atccattcga tggtgcagtc ctccaacgcg
     2641 ttagcttcgc cgagcagcca cacccccggc cgctgcggga acgtggcgcc atagcggaac
     2701 gtgaacccac ggaccaccac gtggtcgaag ctcgcgccct gcatccaagg gttcgcgccg
     2761 aacaccagac cgcgtgtcgc tccctcgatc tggtgcacgg ccggatcgga gtcgtcggcc
     2821 atgcggatgt agaggaaccc agagtcagga tcggggaaga aggtcccggg tgtcatctcg
     2881 gccagggaca gaacgagctg gggatagtcc caaacctcgc cgtcgacgat gacctgctcg
     2941 gctcggccgc tgcgggagtg ttcctcgtca cccggatggt gccgaaccgg cgtcccgtcg
     3001 cgcatgtcga tgacgaactc atgtgtccat ggcacgcggt agatgggcgc atcgccccca
     3061 acgcgcgccc atccggtgag cacatcggcg ccggtgacga tgacggtttc gcccgcggcc
//
LOCUS       DAOSPL010000424         3117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31294_length_3117_cov_3.120509, whole genome shotgun sequence.
ACCESSION   DAOSPL010000424 DAOSPL010000000
VERSION     DAOSPL010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31294_length_3117_cov_3.120509"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PLQ54_08770"
     CDS             complement(<1..642)
                     /locus_tag="PLQ54_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93391.1"
                     /translation="MARAVRRRGNRVWIDGVPILGFSRGIECAYIGALAAALSVTDHP
                     SPYAELMGFSGLAFRVRWWVSPQEPGNRGWCPSTPVGEFPEEGDAIQRNTGWRFRPIA
                     RFTHPGGPHMEELIPDIVASIDAGIPVLAYPSIHNLNMGTIYGYDDGGVVWLLRDYFS
                     VDGMTLVPAPDLGPVVLIPTHWEPPPPRRKALLDSIAMALRHWARRRGPDPDGP"
     gene            complement(787..1848)
                     /locus_tag="PLQ54_08775"
     CDS             complement(787..1848)
                     /locus_tag="PLQ54_08775"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS93392.1"
                     /translation="MAKKLKAAVIGTGMGRFHARSYAANKKVDLVALCDLNKPEAEWL
                     AGELGVPHVFDNYAQMLDSVKLDIVSVVVPNHLHAPMTLDALERGIHVLCEKPLATTV
                     ADGEAMVALAEKQNLRLMVNLCSRFDPVNATVAKMVRDGDLGEVYYVRGGMMRRRGTP
                     RLDFDSSGDLGRGAWFVQAEKAGGGALMDIGVHILDLCWWLAGCPAPVAVSGATFPDF
                     RQAEFAKQKVRGDVDNGAAATIRCENGAQIFFEATWASNMKDEHYCKVYGTKAGASAM
                     GEAMFYTTKAGKPADKPITPIPGITKDSYEHFVDAVLDPSIELNASGAGGLTILRILD
                     AIRQAGRDGADTYLCDCCC"
     gene            2021..>3117
                     /locus_tag="PLQ54_08780"
     CDS             2021..>3117
                     /locus_tag="PLQ54_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93393.1"
                     /translation="MWTASGRAVGDFPGGVGFAVVPPVVVSLTRWTAVFILFLAALSS
                     HAARAALPDGFNHSEYRWKVLETAHFRVIYHQGMESTAEVTAREAEWIYPRVTAIMRV
                     EPGDKTDIIVSDYTSVGIFAGSSYPLSHRIWLYGLTIYGARGDRDDVLRSVLYHEFVH
                     ICTYWAVRRGTFSTLWEGLNSGTLPGWFLEGLAEYVSEKAASPDPEAYDYKGDALVRA
                     AVLEDDVTKLPGLAVEDWDDIYDISLTYRVGQSLVAYIAREFGGDKTLVRLMREHSKF
                     PLFDSACNEVLGIHEEELFRRWRDYTRGYYEELVAGRTDTRESASRVPVPIQDVFGVR
                     WAPHGRALAALGYLDGEEYDLQLFTVRADGTG"
BASE COUNT          498 a          893 c         1167 g          559 t
ORIGIN      
        1 cgggccatcc gggtcgggtc cgcggcgacg ggcccagtgg cggagggcca tggcgatgga
       61 gtcgaggagg gccttccggc gcggcggggg cggctcccag tgagttggga tgaggacgac
      121 gggcccgaga tccggtgcgg gcaccagggt catgccatcg acggagaagt agtcgcggag
      181 gagccacacg acgccgccgt cgtcatagcc gtagatggtg cccatgttga ggttatggat
      241 actggggtag gcgagaacgg gaatgccggc gtcgatggag gcgacgatat cggggatgag
      301 ttcctccatg tggggcccgc cgggatgggt gaagcgggcg atgggacgga agcgccatcc
      361 ggtgtttcgc tggatggcgt cgccctcttc ggggaactca ccgacggggg tggaggggca
      421 ccagccgcgg tttccaggtt cctggggtga aacccaccag cggacccgga aagcgaggcc
      481 ggagaatccc atgagttccg cgtagggtga ggggtgatcg gtgacgctga gtgcggcggc
      541 gagtgcgccg atgtaagcgc actcgatgcc ccggctgaaa cccaatatgg gcacaccgtc
      601 gatccagacc cggttgcctc tgcgccgaac tgcccgcgcc atggccggac gacccttccg
      661 tcgcacgtgg tgccatcggt acccgtattc gctaatccat agcgtattgc ctgcctattg
      721 gcgtggtggg cctcgggatg gagagggatt cccgaggccc atcttggatt tgaagtaagg
      781 gagcgatcag cagcagcagt cgcagaggta ggtgtcggcg ccgtcccggc cggcctggcg
      841 gatggcatcg aggattcgga ggatggtgag gccacccgcg ccggacgcgt tgagctcgat
      901 ggacgggtcg agcacggcat cgacaaagtg ctcgtacgag tccttggtga tgcccgggat
      961 gggtgtgatg ggcttgtcgg cgggcttgcc ggctttggtg gtgtagaaca tggcttcgcc
     1021 catggcgctg gctccggcct tggtgccgta gaccttgcag tagtgctcgt ccttcatgtt
     1081 ggaggcccaa gtggcctcaa agaagatctg ggcaccattc tcgcagcgga tggtggctgc
     1141 ggcgccgttg tcgacatcgc cgcgcacctt ctgcttggcg aactcggcct ggcggaagtc
     1201 ggggaaggta gcgccggaga cggccacggg ggcggggcat ccggccagcc accagcagag
     1261 gtcgaggata tggacgccga tgtccatgag agcgccgccg cccgctttct cggcctggac
     1321 gaaccaggcg ccgcggccga ggtcgccgct ggagtcgaag tcgaggcggg gagtcccgcg
     1381 gcggcgcatc ataccgccgc gcacgtagta gacctcgccc aggtcgccgt cgcggaccat
     1441 ctttgcgacg gtggcattga cggggtcgaa acggctgcag aggttgacca tgaggcgcag
     1501 gttctgcttc tcggccaggg cgaccatggc ctcgccatcg gccacggtgg tggcgagggg
     1561 cttctcgcag agaacatgga tgcctctctc gagcgcgtcg agggtcatgg gggcgtggag
     1621 atggttgggg acgacgacgc tgacgatatc gagcttcacg gagtcgagca tctgggcgta
     1681 gttgtcgaac acatggggga ctccgagctc accggcgagc cattcggcct cgggcttgtt
     1741 gaggtcgcag agggctacga ggtcgacttt cttgttagcg gcgtagctgc gggcatggaa
     1801 ccggcccatg ccggtaccga ttacggcggc tttgagcttc ttggccatca gggtctcctc
     1861 tagacgcacg cggtgtgggg caagactgtg cccgtaagtg ttcgggtcgg acgccgggac
     1921 tccttgcggg ggcaggggat gcccggcatc gtcccgaaag cgctggcgcg gcggccggct
     1981 tgggcgaggt ccgcgacccc agtataatgg cgcgatgcgt atgtggactg caagcggccg
     2041 agctgtcggc gacttcccgg gtggcgtggg gttcgccgtc gtcccgccgg tcgtggtgag
     2101 cctcacccgt tggaccgcgg tgttcatcct cttcctcgcg gcgctgagct cgcatgcggc
     2161 gcgggcggct ctcccggatg ggttcaacca cagcgagtac cgctggaagg tgctggagac
     2221 ggcgcacttc cgcgtgatct accatcaggg catggagtcc acggccgagg taacggcacg
     2281 tgaggctgag tggatctacc cgcgcgtcac ggccatcatg cgggtcgagc caggcgacaa
     2341 gaccgacatc atcgtcagcg actacacatc ggtggggatc ttcgcgggca gttcgtaccc
     2401 gctgagccac cgtatctggc tctacggcct gaccatctac ggtgcccggg gcgaccggga
     2461 tgacgtactg cgctcggtgc tgtaccacga gttcgttcac atttgcacct actgggcggt
     2521 gcgtcgcggg acgttcagca cgttgtggga gggtctgaac tcgggcacac tgcccggctg
     2581 gttcctcgag gggctggcgg agtatgtgtc ggagaaggcc gcgagtcccg atccggaggc
     2641 ctatgactac aagggcgacg cgctcgtgcg tgcggccgtg ctcgaggatg atgtgacgaa
     2701 gctgccgggt ttggcggtcg aggactggga cgacatctac gacatctcgc tcacctaccg
     2761 cgtggggcag tcgctggtgg cgtacatcgc tcgcgagttt ggcggcgaca agacactggt
     2821 gcgcctcatg cgtgagcact cgaagttccc gctgttcgac tcggcgtgta acgaggtgct
     2881 cggcattcac gaggaggagc tcttcaggcg ctggcgcgac tacacgcgcg ggtactatga
     2941 ggagctcgtc gcgggacgga cggacacccg ggagtctgcg tcgcgagtgc cggtgccgat
     3001 tcaggacgtg ttcggggtgc gatgggcgcc gcatgggcgg gctctcgccg cgctgggtta
     3061 cctggatggc gaggagtatg acctgcagct cttcactgtg agagccgacg gcaccgg
//
LOCUS       DAOSPL010000425         3109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31392_length_3109_cov_3.224296, whole genome shotgun sequence.
ACCESSION   DAOSPL010000425 DAOSPL010000000
VERSION     DAOSPL010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31392_length_3109_cov_3.224296"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PLQ54_08785"
     CDS             <1..462
                     /locus_tag="PLQ54_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOS93394.1"
                     /translation="RVYVSTTDPEAGEKAVQFINTLVRDPEIGEVYDGTVTRLMSFGA
                     FVELVPGKEGLLHVSEYAWERTPSIADVLKVGDPVRVKVSEIDDQGRINVSRKQLLDR
                     PEGVSDSPSSSRGREGGDHPRTPRRRGGSAGREEGPRGGAGGGRTRFREKR"
     gene            459..1709
                     /locus_tag="PLQ54_08790"
     CDS             459..1709
                     /locus_tag="PLQ54_08790"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOS93395.1"
                     /translation="MTRCDTLANGIRVVTDRMEHVATATVGFYVAAGSRHERAEESGV
                     SHFIEHLVFKGTPTRSPRDIVLAFDAVGGDVNAATEREITYYYGKVLGQHVPMAIEVL
                     SDMLLHPKLAEEDVDLELLVIEEEISRYEDTPSELVHDQFAKALWPGQAIGRSILGDV
                     ECLRALGPEGVRSYMTGHYTGPNIVVSAAGDVHHEQVVDLIAAHWSELDVPSSGPAAL
                     PAPVASPDVVCDERDIEQVHFCLGGEAYSMRDEGRYAGAIIDCALGGAASSRLFQEIR
                     EKRGLVYSVYSSTAMHLDSGYESIYAGTRPENLTRVLELIREQFADLLAHGLSSEEVR
                     AAKEYHRGATLMALDGVGHRAARNARSLIAFDRVVPTQEVIAGIEAVTTDQVHEAIHR
                     LFASKPCLAAIGPVKRSDLAAAVL"
     gene            1791..2567
                     /gene="dapB"
                     /locus_tag="PLQ54_08795"
     CDS             1791..2567
                     /gene="dapB"
                     /locus_tag="PLQ54_08795"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOS93396.1"
                     /translation="MSVRVVVSGAAGRMGQETCRAVASDPALELVGAVDVVQSGRSLA
                     DLVGGGVPGLPMRGDLVAVVRDTQAQVVVDFSVPESRMKVFSAAIAAGARPVVGTTGW
                     TEADIAACRTLCQRHGLGAIIAPNFSIGAVLMMKFAAQAARYMPSVEIIELHHNQKLD
                     APSGTAVKTAALIAAARGDREPGEPHPARGQEFHGVPIHSVRLPGLLAHQQVIFGGLG
                     QTLTLVHDSLSRESFMPGVILACKRVLELNELVYGLENLL"
     gene            2602..>3109
                     /locus_tag="PLQ54_08800"
     CDS             2602..>3109
                     /locus_tag="PLQ54_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HOS93397.1"
                     /translation="MGFRVAVVGIGAVGQTMLRVLRERGFPADHVKVLARRERIETVD
                     GVDYPVRAISEAEFDDVDVALFAGTEGVGGASETFAPAAVARGCFVIDNSSAFRMFPN
                     VPLVVPEVNFHAIGPQHRHIANPNCSTIQMVVALAPLHRAARIRRIVVSTYQSVSGTG
                     LAAIDELMA"
BASE COUNT          501 a         1003 c         1093 g          512 t
ORIGIN      
        1 cgcgtatacg tgtcgacaac ggacccagaa gcgggcgaga aggccgttca gttcatcaac
       61 acgctggtcc gcgaccccga gatcggcgag gtgtacgacg gcacggtcac acgcctcatg
      121 agcttcggcg ccttcgtcga gctagtaccc ggcaaggagg ggcttctgca cgtatcggaa
      181 tacgcctggg aacgcacgcc cagcattgcc gacgtgctca aggtcggcga cccggtacgt
      241 gtgaaggtca gtgagatcga tgatcagggc cggatcaacg tcagccggaa gcagctcctg
      301 gatcggccgg agggtgtgag cgatagcccc agttcctcgc gcggccgtga gggcggagac
      361 catccccgca cgccgcgacg gcgcggcgga tccgcaggcc gcgaggaggg tccgcgcggt
      421 ggggccggtg gcggccgcac gcgtttccgg gagaagcgat gacccgctgc gacaccctgg
      481 cgaacggcat ccgagtggtc accgaccgga tggaacacgt ggcgacggcc accgtgggct
      541 tctacgttgc tgccggatca cgccatgagc gagccgagga gtcgggcgtc tcccatttca
      601 tcgagcacct ggtgttcaag gggacgccca ctcgcagccc tcgcgatatc gtcctcgcgt
      661 tcgacgccgt gggcggcgat gtcaacgcgg ctaccgagcg ggagatcacc tactactacg
      721 gcaaggtcct gggccagcac gtgcccatgg ccatcgaggt gctcagtgac atgctgctgc
      781 acccgaagct ggctgaggag gacgtcgacc tcgagctcct ggtgatcgag gaggagatca
      841 gccgctacga ggacacgccg tcggaactgg tgcacgacca gtttgccaag gctctatggc
      901 ccggccaggc catcggccgc tcaatcctgg gtgacgtcga gtgcctgcgg gcgctgggac
      961 cggagggggt ccgcagctac atgacggggc actacacggg ccccaacatc gtggtatcgg
     1021 ctgcgggcga tgtgcaccac gagcaggtcg tcgacctgat cgccgcacac tggtccgagc
     1081 tcgacgtgcc ttcctccggg cccgcggcgc tcccggcgcc cgtggcatcg cccgacgtcg
     1141 tctgtgacga gcgtgacatc gagcaagtgc acttctgcct gggcggcgag gcctactcga
     1201 tgcgcgacga ggggcgctac gctggggcca tcattgactg cgcgctgggc ggggcggcta
     1261 gctcgcgctt gttccaagag atccgtgaga agcgcggact ggtctacagc gtctactcgt
     1321 caacggccat gcacttggac tcaggctacg agtcgatcta tgcggggacc cggccggaga
     1381 acctgacccg ggtcctcgag ctgatccgcg agcagtttgc cgatctcctg gcgcacggtt
     1441 tgtcctccga ggaggtccga gcggcaaagg aataccaccg gggcgcgacg ctgatggccc
     1501 tcgacggcgt gggtcaccgg gccgcgcgca acgcccggtc gctgatcgcg ttcgatcggg
     1561 tcgtgccgac ccaggaggtc attgcgggga tcgaggctgt gaccaccgac caagtgcatg
     1621 aggccatcca ccgtcttttc gccagcaagc cgtgcctggc ggcgatcgga ccggtcaaac
     1681 ggagtgacct ggccgccgcg gtgttgtaga ccgcggcggc ggtcgcgccc tggcgcctcg
     1741 atcgccatct ccccgggctc atccccagtc tcgctagagg gaggaccacc atgtcggttc
     1801 gtgtcgtcgt gtcgggcgcc gcggggcgga tgggccagga gacatgccgg gccgtggcct
     1861 ccgaccccgc cctcgaactg gtgggcgccg tggacgtcgt ccagtccgga cgcagcctcg
     1921 ccgatctggt gggcggaggg gtgcctgggt tgcccatgcg aggcgacctg gtggccgtcg
     1981 tgcgtgatac gcaggcacag gtcgtggtcg acttcagcgt gcccgagtcg cgtatgaagg
     2041 tgttctcagc cgccatagcg gcgggcgccc gcccggtggt gggaaccacg ggctggaccg
     2101 aagccgacat tgccgcgtgc cgcacgctgt gccagcggca cgggctggga gcgatcatcg
     2161 cgccgaactt ctccatcggc gcggtgctca tgatgaagtt tgcggcccag gccgcccggt
     2221 atatgcccag cgtcgagatc atcgagttgc accacaacca gaagctcgat gcaccctccg
     2281 gcacggcggt gaagaccgcg gcgctcatcg ccgcggcgcg cggtgatcgg gagccgggcg
     2341 agccgcatcc ggcacgaggt caggagttcc acggtgtgcc catccacagc gtcaggctcc
     2401 ccggtctcct ggcccatcag caggtgatct tcgggggtct gggacagacc ctgacgctcg
     2461 tccatgactc gctgtcacgc gagtcgttca tgcccggcgt catcctcgcg tgcaagcggg
     2521 tgctggagct gaacgaactg gtctacgggc tcgagaatct tctgtaggaa atcacgaggt
     2581 cgtgaagggg aggttggggc catgggcttc agggtagcgg tggtggggat cggcgcggtg
     2641 gggcagacaa tgctccgggt gctccgcgag cgcggatttc cggccgacca tgtcaaggtg
     2701 ctcgctcgac gcgagcgcat cgagaccgtc gacggagtgg actacccggt ccgggcgatc
     2761 agcgaagctg agttcgacga tgtggacgtc gccctgttcg cgggaaccga gggggtgggg
     2821 ggcgccagcg agaccttcgc tccggccgcg gtcgcgcgcg gatgcttcgt gatcgacaac
     2881 tcgtcggcat tccgcatgtt cccgaatgtc cctctggtgg tacctgaggt gaactttcac
     2941 gccatcggcc cgcagcatcg gcacattgcc aacccgaact gctcgaccat tcagatggtc
     3001 gtcgccctgg caccgctgca ccgcgccgcg cggattcgcc gcatcgtcgt ctccacctat
     3061 cagtcggtgt cgggcaccgg cctggcggcc atcgacgagc tcatggcgc
//
LOCUS       DAOSPL010000426         3106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31431_length_3106_cov_4.032448, whole genome shotgun sequence.
ACCESSION   DAOSPL010000426 DAOSPL010000000
VERSION     DAOSPL010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31431_length_3106_cov_4.032448"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..1487
                     /locus_tag="PLQ54_08805"
     CDS             36..1487
                     /locus_tag="PLQ54_08805"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HOS93398.1"
                     /translation="MNPHGCEIPMYWWSNTGMLEHEGGRVITPADTTYHGLTVHDCPV
                     INGLDYSYTTRVRRAYDLFFRIPPDRRPWEVYVDRDGRGYVHTSTRRLRGRKIFAWGM
                     AQGGRRWGEYLAVENMPYVEIQAGLAYTQFHTARMPAKTEWSWTEAMGYFEADATRLH
                     SENWQEAYAEAGSVLERMLPEAELDQTHAALAAVATSTPEEILYRGHGWGALEKLRAQ
                     AAGEDSGIPPELPFDAADLGPDQEPWLQLLETGVFPPRGVDEDPGQFQVQPEWERLLR
                     ASVESGKSDHWLAWLHLGVMAMEAGDTERARLAWETSHARTPNGWALRNLAVIESRAG
                     NDEAAGELLARAVKTGPAVVPLVAEYASVLLRRKEFEAVDALLASLPTEVRQAERLRL
                     AGGWAALHAGRFEEVEEVLAGDFATIQEGEVSLSELWFGLQERRLAEAEGVEVDEDLK
                     ARVRRDFPPPYEIDFRMSAEGDDKYVPPQAAGG"
     gene            complement(1682..2869)
                     /locus_tag="PLQ54_08810"
     CDS             complement(1682..2869)
                     /locus_tag="PLQ54_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93399.1"
                     /translation="MCEAHIGETDHFWYRLQPEGLYIDSQRDGKAFAHAPGRVMLSED
                     CGRTWPHSLAFADADNVTFSCILRNGNVVFATRERLYLSTDSLRTCREIIVQGLDGAD
                     YLPHTPRNPDCPGWYYHTIPGVNTFDVHGTEMLIWGNYCNVVGGAAPVNIYYSVDGGE
                     TVRIAYAFGQSPYTRDDGSPGGGTEGTPLGNPDNPLCCRHIHTVAYNPWEDAFYTCTG
                     DHDQPEGLECHWLRGTYDWAADRWDWQLLISDHLNSRYKAGGITFVDGQLYWISDSNG
                     PEPYDRGIFRCAPEDLARPEKHTRLFNPEVESGNMIIQDGVFLASHCAPASPLDTGII
                     ISTDGGETFAQYDLKEFGKRSPTRLHHKNSEGWFRMDLRSGWVEYADVLFLKPKTTGS
                     RLV"
BASE COUNT          502 a         1025 c         1058 g          521 t
ORIGIN      
        1 gggagtgatg acgcggccgc cttcgtgttc gcctcatcaa cccccacggt tgcgagatac
       61 ccatgtactg gtggtccaac accgggatgc tcgagcacga aggcggccgc gtcatcactc
      121 ccgcggacac cacttaccac ggcttgacgg tccatgactg cccggtcatc aacggcctcg
      181 attacagcta cacgacccgc gtccgccgcg cgtacgacct attcttccgc atccctcctg
      241 accggcgccc gtgggaggtc tacgtggacc gcgacggtcg cggctatgtc cacacctcca
      301 cgcggcgcct tcgcggccgc aagatcttcg cctggggcat ggcgcagggc ggacgacgct
      361 ggggcgagta cctcgcggtt gagaacatgc cctatgtcga gatccaggcc ggtcttgcct
      421 acacccagtt ccacacggcc cggatgcccg cgaagaccga gtggtcctgg actgaggcca
      481 tgggctactt cgaggccgat gccacacgcc tccattccga gaactggcaa gaggcctatg
      541 ccgaagccgg cagcgtcctc gagcggatgt tgcccgaggc ggagctggac cagactcacg
      601 ccgccctcgc ggccgttgcc acgtcgactc ccgaggagat cctctaccgc ggtcacggtt
      661 ggggcgcgct cgagaagctc cgcgcccagg ccgccggcga ggactcgggc atcccccctg
      721 agctgccttt cgacgcggct gacctgggcc ccgaccagga gccctggctg cagttgctcg
      781 agacgggcgt cttcccgccg cgtggggtcg atgaggaccc cggccagttc caggtccagc
      841 ccgagtggga gcgactcctc cgggcgtctg tggagagcgg caagagcgat cactggctcg
      901 cctggctgca cctgggcgtg atggccatgg aggctggtga cacggaacgc gcccggttag
      961 cctgggagac ctcacacgcc cgcacgccca acggctgggc tttgcggaat ctggcggtga
     1021 tcgagtcgcg tgcgggcaac gatgaggccg cgggcgagct tctcgctcgc gcggtcaaga
     1081 cggggccagc ggtcgtgcct ctcgtggccg agtatgcctc ggttctcctg cggcgcaagg
     1141 agttcgaggc cgttgatgcg ctgctcgcgt ccctgcccac cgaggtgcgc caggccgaac
     1201 gcctccgcct ggcggggggc tgggcggcgc ttcacgccgg acggttcgag gaggtcgaag
     1261 aggtactcgc gggcgacttc gccacgatcc aggagggcga ggtgagtctg agcgagctgt
     1321 ggttcggcct tcaggagcgc cgcctggccg aggccgaggg tgtcgaggtt gatgaggatc
     1381 tcaaggcacg cgtccggcgc gacttcccgc cgccctacga gatcgacttc cgcatgtccg
     1441 ccgagggcga cgacaagtac gtaccgcccc aggccgccgg cggctgagcc tggggccgcc
     1501 atcacggtcc ggagcgatga cgccagggag atctccgctg cacagtcggc gggacccgaa
     1561 ctaccggcgt acgggcctgg gcgccgaggg ctaccgctac gaccttgccc atggcgaggc
     1621 ggtgtgcatt gtcggcagcg gcctgcagca agtgcccggt gccggtcacg accccgggcg
     1681 gctacaccag gcgacttccc gtggtcttcg gcttcaggaa cagcacgtcc gcgtactcga
     1741 cccagcccga ccggaggtcc atgcggaacc aaccctcgct gttcttgtgg tgcaggcgcg
     1801 tgggcgaccg tttgccgaac tccttcaggt cgtactgcgc gaacgtctcg ccgccgtcgg
     1861 tcgagatgat aataccagta tcgagcggcg aggccggcgc gcagtgtgag gcgagaaaca
     1921 cgccatcctg gatgatcatg ttccccgact cgacctcggg gttgaagaga cgggtgtgct
     1981 tctccggccg ggccagatcc tcgggcgcac accggaagat gcctcgatcg tagggctcgg
     2041 ggccgttcga gtcactgatc cagtagagct gtccatcgac gaacgtgatc ccgcccgcct
     2101 tgtagcgcga gttcaggtgg tccgagatca gaagctgcca gtcccaacgg tccgccgccc
     2161 agtcataggt gcccctcagc cagtggcact caagcccttc tggctgatcg tggtcccccg
     2221 tgcacgtgta gaaggcatcc tcccatgggt tgtaggccac cgtgtggatg tgccgacagc
     2281 agagtgggtt atccgggttg ccgagcggcg tgccctcggt tcctccgccc ggagagccgt
     2341 cgtcgcgggt gtaggggctc tgaccgaagg cataggcgat cctcaccgtc tcgccgccat
     2401 cgaccgagta gtagatgttg accggcgccg ctccacccac cacgttgcag tagttgcccc
     2461 agatgagcat ctcggtgccg tgcacgtcga acgtattcac gccggggatg gtgtggtagt
     2521 accagcctgg gcaatcggga ttccgcggcg tgtgggggag gtagtcggcg ccgtcgaggc
     2581 cctggacgat gatctcgcgg caggtccgca ggctgtccgt gctgaggtag agccgctcgc
     2641 gcgtggcgaa gaccacgttt ccgttcctca ggatgcaact gaacgtgacg ttgtccgcgt
     2701 cggcgaaggc aaggctatgg ggccacgtgc ggccacaatc ctcagacagc atgactcggc
     2761 ccggcgcatg ggcgaaggcc ttgccgtcgc gctgtgagtc gatgtagagg ccttctggct
     2821 gcaggcgata ccagaagtgg tctgtctcgc cgatgtgggc ttcgcacatc acgaagcact
     2881 cccttgggat tcggtagggt gtataccacc tcggcgagtt ccattggcgg ggtagggcga
     2941 cctctcgggg cacgaggagc catccaacct gtggaggcaa cgcgcgagtc tcggagcgct
     3001 ccgcttccac gaggacaccg cccccatgcc gccgaacgcc cgcctacgga tagggggcgc
     3061 cacaacatga gcagcgccat caggatgggc tgggcgcacc gggaca
//
LOCUS       DAOSPL010000427         3099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31517_length_3099_cov_3.752628, whole genome shotgun sequence.
ACCESSION   DAOSPL010000427 DAOSPL010000000
VERSION     DAOSPL010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31517_length_3099_cov_3.752628"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..>3099)
                     /locus_tag="PLQ54_08815"
     CDS             complement(22..>3099)
                     /locus_tag="PLQ54_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93400.1"
                     /translation="GGHAMTAPAARVALVGIALSVLCPGAPAAGEIWLGPDEPSPFVP
                     DAARWIHAPQTPSEPWDHWAYFRVDVQVPAKVQRAVLALAPNQYVELCLDGQLVSTAY
                     DHHECLPVFTDVTRRLAPGTHRLAARVYSEWMPALYAQLRMEGTDGRVRDQCTGVDWD
                     SCLDAGDGWRHATEGSWSPARDGGGYHWRPGDSWIGREFALLPRQQVREHFEAHNSRL
                     RDSWREEQRQPRSEFRGRYEKPEYADRYRRFRTLDRRTRQLLDADGREIHPFFTIYGQ
                     IRDGTTVLSIESFDFDQLERDLDLMERAHVNPYMRNLGWHVLLDEHGQWAKVKAQPPG
                     SDLPEFRLMRDVWDYFLDRVEAHGLHVVLEADFYWSANGGVVWAPYRSRYHLYPEALE
                     AQALATRKIMRYFSSRPCVMAYMAGEEDIGLAHDMTSPHVAEAYHRHLERQYGTVERF
                     RETHPGNYDTRKPARLKPASRTPEFIAKSPPLEVLVPEYDWDPGAFEGLTSLADVPLP
                     VWPRFLMPEAPEVAATAHRSYNDFTPDDPLWIDYFETAEEQQLFDMLVRWATIVREGC
                     PDQLLFYSNAQDFTANWHFLHLLRRAELPFDVVGVGCHDSGIEPQDLEPWATTRKAIK
                     NVSAYRPFVYARGARPVAIASGEGEGGHTPKGVLDYYRGGLFDEIGGGLAWTQTYNWL
                     HMSGAVAGAGPHETPLLAWMAEFMPEHERDPFPLRGQAPILIVENANLQHSNRSGLNY
                     GNVLSVAHYLTQLNVDFDIVNDQAIGYGRGEKLKVNLSPYRLIILPALACDLDPAGIE
                     AIDRWLSDRRFAGRRTVCLGLSENRTPYLYPHPGLHPTLAAWLGIADYESRAELSGTQ
                     RLYCPRRIGSLRPGEAISLTLAGGSRCAFFAPDHALLTLEDGRAIATSATYAGNRVIG
                     FGLFLGRNYSDLWAADPRQTPYDAPTALFEAMVDEAGIDRPIRAPHHVRVYPSADGRT
                     LLIRERYSLATDAAIEVRLPEGVRYAGGQRLGYGRTRFEVHLEPYEGIVLSAE"
BASE COUNT          506 a         1026 c         1084 g          483 t
ORIGIN      
        1 ggagccggtc tccggggtcg cctactccgc gctcagcacg atgccctcgt agggctcaag
       61 gtgcacctcg aagcgcgtgc gcccgtagcc caggcgctgt cctccggcat agcgcacgcc
      121 ttcgggcaga cgcacctcga tggccgcgtc ggtcgccagc gagtagcgct cgcggatcag
      181 cagcgtgcgc ccatcggcgc tcggatagac tcggacatga tgcggcgcac ggatgggacg
      241 gtcgatgccg gcctcatcga ccatggcctc gaagagggcc gtcggtgcgt catagggggt
      301 ctggcggggg tccgccgccc acagatcact gtagttgcgc ccaaggaaca ggccgaagcc
      361 gatgacgcga ttgcccgcgt aggtagccga cgtggcgatg gctctcccat cctccagcgt
      421 cagcagcgcg tgatcgggcg cgaagaaggc acagcgggaa ccgccggcga gcgtcagcga
      481 gatggcctcg ccgggcctca gggagccgat acgccgcggg cagtagagcc tctgggtccc
      541 cgagagctcc gcgcgcgact cgtagtccgc gatcccgagc cacgcggcga gggtggggtg
      601 aaggccgggg tgcgggtaca ggtagggcgt ccggttctcc gagaggccca ggcacaccgt
      661 cctccggccc gcgaagcggc ggtcggacag ccaccggtcg atggcctcga tcccggcggg
      721 atcgaggtcg cacgccaggg cgggcaggat gatcagccgg taaggactca ggttgacctt
      781 gagcttctcg ccacggccat agccgatggc ctggtcgttg acgatatcga agtcgacatt
      841 gagttgcgtc aggtagtgcg ccactgacag cacattgccg tagttgagcc cgctgcggtt
      901 cgagtgctgc agattcgcgt tctccacgat caggatgggc gcttggccac gcagcgggaa
      961 cgggtcgcgc tcatgctcgg gcatgaactc ggccatccac gccagcagcg gcgtctcgtg
     1021 gggccccgcg ccggccacgg ctccggacat gtgcagccag ttgtaggtct gtgtccacgc
     1081 cagcccgccg ccgatctcat cgaacaggcc gccccggtag tagtcgagca ctcccttggg
     1141 cgtatggcct ccctcgccct cgccggacgc gatggccacc ggccgcgcgc cgcgcgcgta
     1201 cacgaaggga cggtaggccg agacgttctt gatggccttg cgcgtcgtgg cccacggctc
     1261 caggtcttgg ggctcgatgc cggagtcatg gcagcccacc ccgaccacat cgaaaggcag
     1321 ctcggctcgc cgcagcagat gcaggaagtg ccagttggcc gtgaaatcct gcgcgttcga
     1381 gtagaacagg agctggtcgg gacagccttc gcggacgatg gtcgcccagc gcaccagcat
     1441 gtcgaacagc tgctgctctt ctgcggtctc gaagtagtcg atccacagcg ggtcatccgg
     1501 cgtgaagtcg ttgtaggacc ggtgggccgt ggccgccacc tcgggcgcct cgggcatgag
     1561 gaaccggggc cagaccggca gggggacatc ggccaggctc gtcaggccct caaatgcacc
     1621 gggatcccag tcgtactcgg gcaccaggac ctcgagcggc ggcgatttgg cgatgaactc
     1681 gggcgtgcgc gacgcgggct tcagcctggc cggcttgcgg gtgtcgtagt tgcccggatg
     1741 agtctcgcgg aagcgctcca ccgtaccgta ctggcgctcg agatgcctat ggtaggcttc
     1801 ggccacgtga gggctcgtca tgtcatgggc caagccgatg tcctcctcgc cggccatgta
     1861 ggccatgaca caggggcgag acgagaagta ccgcatgatc ttgcgcgtgg ccaacgcctg
     1921 cgcctcgagc gcttcggggt agaggtggta ccgcgaccga tagggtgccc acacgacccc
     1981 accgttcgcc gaccagtaga agtcggcctc gagcaccacg tgcaggccgt gggcctcgac
     2041 gcggtcgagg aagtagtccc acacatcgcg catcagccgg aactcgggca gatccgaccc
     2101 tgggggctgg gccttcactt tggcccactg cccatgctca tccagcagca cgtgccaacc
     2161 caggttccgc atgtaggggt tcacatgcgc ccgctccatc aggtcgaggt cgcgctccag
     2221 ctggtcgaag tcgaagctct cgatgctgag gaccgtcgtg ccatcgcgga tctgaccgta
     2281 gatggtgaag aagggatgga tctcgcgccc gtcggcatcg agcaactgcc gtgtgcggcg
     2341 gtcgagtgtg cggaagcggc ggtaccggtc cgcgtactcg ggcttctcgt agcggccgcg
     2401 gaactcggag cgcggctggc gctgctcctc ccgccatgag tcccgcaggc gcgagttgtg
     2461 ggcctcgaag tgctcgcgaa cctgctggcg cggcagcagg gcgaactcgc ggccgatcca
     2521 gctgtcgccc ggacgccagt ggtacccgcc tccatcgcgg gcgggagacc acgatccctc
     2581 ggtggcgtga cgccagccgt ctcccgcgtc gaggcacgaa tcccagtcca cgcccgtgca
     2641 ctggtcgcgc acacgcccgt ccgtgccctc catccggagc tgtgcgtaga gcgcgggcat
     2701 ccactccgag tacacccgtg ccgccaatcg atgggttccc ggcgcaagcc gtcgggtcac
     2761 atcggtgaag accggcaggc actcgtggtg gtcataggcc gtggacacga gctgaccatc
     2821 gaggcacagc tccacgtact ggttgggcgc cagggcgagt acggcgcgct gcaccttggc
     2881 gggcacctga acgtctacac ggaagtaggc ccagtggtcc catggttcac tcggtgtctg
     2941 gggcgcatgg atccagcggg cagcgtcggg gacgaagggt gacggctcgt ccgggccgag
     3001 ccagatctcg cccgccgccg gggcgccggg acacagcacc gacagggcaa tgccaacgag
     3061 agcgacgcga gccgcaggtg cggtcatggc gtgacctcc
//
LOCUS       DAOSPL010000428         3097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31548_length_3097_cov_2.732084, whole genome shotgun sequence.
ACCESSION   DAOSPL010000428 DAOSPL010000000
VERSION     DAOSPL010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31548_length_3097_cov_2.732084"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..695)
                     /locus_tag="PLQ54_08820"
     CDS             complement(123..695)
                     /locus_tag="PLQ54_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOS93401.1"
                     /translation="MAGMVEVRWHGRGGQGAKTAAYLLAEAVMGEGQNFQAFPEYGPE
                     RMGAPIRAFVRISDTPIRMHCPVTSPGVVVVLDPTLIGKVDVLEGVPEDGTIIINSDQ
                     SPQELAKLLGAGNRKVWTVAATQIAMDTIRRPIPNTVMLGALARATGLLDLERLKSKV
                     THKFGTKLKAEVLQGNLTAIDRAYQEVQST"
     gene            complement(1243..2202)
                     /locus_tag="PLQ54_08825"
     CDS             complement(1243..2202)
                     /locus_tag="PLQ54_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93402.1"
                     /translation="MNQRVLEAMVLVCLAVGLCQADVTDQHRQQAALAAVRRAAGTAK
                     AVTGIKRFPPSTWVIVDGRWHVTFLDGSGEVRSVADDDFAKSEHARHVLNESPESAEI
                     LRVGQKLVQRVFPGIDMREMTLTSTEVVAGTGRVRAGWARVISENGFRDSATCRVEYC
                     WQDRAIFEVAARCPPTPDEWRRPETVTAAQAEEAARRALGDLGGGWLEVCKDRCVINR
                     TNVRPAWQVYLRRRWYGAHGGTYVDRGVWVDPWTGEVLRTATFDSAAGAPGQAPRSPI
                     GPATTDMPAHAAPRPWVPWAGGAAALLVLVLSVALVCLRRRRG"
     gene            complement(2199..2345)
                     /locus_tag="PLQ54_08830"
     CDS             complement(2199..2345)
                     /locus_tag="PLQ54_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93403.1"
                     /translation="MHCLCDWGMTVNEAIKHMKDTDPEWDIHGKYNVWISGGGHRGLW
                     EVQT"
     gene            complement(2365..>3097)
                     /locus_tag="PLQ54_08835"
     CDS             complement(2365..>3097)
                     /locus_tag="PLQ54_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93404.1"
                     /translation="VAHTNVVTDATPPSTLAATVVRTVPIEPVASSALVCSPGEHTDY
                     VPNVGPLEAAGTYWVAVTGYDGHGGVSKYAGNVEHPVEPALVSFGIPSATCIAGDNQG
                     GAPYNDARARAQEWYEALGLVDAPSQQPSDAEFFCTRYEYGPQGPVARGGYQVEPTKA
                     ETLEALFTDDVVVFNGHGTFDAIFNRHSDPTTAQDVLDRAQTAPSQARFVFLKCCLSA
                     TDVRTGPVVQRGPLRRRFRWRDRFG"
BASE COUNT          504 a         1068 c         1000 g          525 t
ORIGIN      
        1 gtcttcatgc cagacgggcc gcatggcgcg ccagtcaccc gtgtggaact cgagggccgt
       61 ggcgggttcg ttgatgaagc caccggcggg cagatccttc cagccggcac ccggtttgat
      121 cttcacgttg actgtacctc ctggtaggcg cggtcgatgg ccgtcagatt gccctggagc
      181 acctcggctt tgagcttggt gccgaacttg tgggtgacct tgctcttgag ccgctcgagg
      241 tcgagcaggc cggtcgccct ggccagcgcg cctagcatca ccgtgtttgg aatgggccgg
      301 cggatggtgt ccatggcgat ctgtgtggcc gccaccgtcc acaccttgcg attgccggca
      361 ccgaggagtt tggccagctc ctgcggcgac tgatcgctgt tgatgatgat ggtgccatcc
      421 tcgggcacgc cctccagcac atcgactttt ccgatgagtg tggggtcgag cacgacgacg
      481 acacccggag acgtgactgg gcagtgcatg cggatcgggg tgtcactgat ccggacgaag
      541 gcgcggatgg gcgcccccat acgctcgggg ccgtactcgg ggaacgcctg gaagttctgg
      601 ccctccccca tgactgcttc cgccagcaag tatgcggcgg tcttggcgcc ctgaccaccg
      661 cgtccgtgcc agcgtacttc gaccatgccc gccatgagca tcctcccact ggagtggcta
      721 gtctacagcc gcccgcgcgg gggcggccac gggttacgga acagcccgtc cccggagccc
      781 aatcacctca cggcgagcag cccacgccat cgccgattgg ggcgggcgca ccaggggtct
      841 ggcaccacta cactagcagg ggaggctaaa tggcaaataa gaacatctct gtcgcgggca
      901 ccaaccgcac cagtagtttc gtgggccaca agagcccatc ttgggcagac gatagggtta
      961 ggtgctgcgc gcagcctctc tcctgcagtg ccggagagcc cgaccgacaa agggtaccct
     1021 ccgcctgagg gtgtgtcaac agcaggggcg aggggtcagg ccctccgcgc tctagaacca
     1081 gcctacgcgc gcgccggagc gcggacggcc tcgtttgcgg tgtcgagcgg gacgcccaac
     1141 ctacgcgccg atggctgagc caggtccttt gacaagctca ggacggtcgg gtcccgcctg
     1201 ccaagttgct ccgcaagccc gtggagtgcg ttgcgtgcgg cctcagcccc tccttcgccg
     1261 caggcagacg agggccacgc tgagcacgag caccagcagt gcggcggctc cgccggccca
     1321 ggggacccac gggcgcggcg cggcatgtgc cggcatgtca gttgtggctg ggccgatagg
     1381 gctccgcggc gcctgacccg gtgcaccggc cgcgctgtcg aaggtcgccg tacgcagtac
     1441 ctcgccggtc cacggatcca cccacacgcc gcgatcgacg taggttcccc catgggcacc
     1501 gtaccaccgg cgcctgaggt acacctgcca ggcgggccgc acgttcgttc ggttgatgac
     1561 gcatcggtcc ttgcatactt ccagccaccc cccgcccagg tcgccgagcg cgcggcgggc
     1621 ggcttcctca gcctgagcgg ccgtcacagt ctcggggcgc cgccactcgt cgggagtggg
     1681 tgggcagcgt gccgcaacct cgaagatggc acggtcctgc cagcagtact cgacccggca
     1741 cgttgcgctg tcgcgaaacc cgttctcgga gatgacccgt gcccagcccg ctcgcacacg
     1801 ccctgttccg gcgaccacct cggtggacgt cagtgtcatc tcgcgcatat cgatcccggg
     1861 gaagacacgc tgtacgagct tctggccaac gcggaggatc tcggccgact ctggtgactc
     1921 attgaggaca tgacgggcgt gctccgactt ggcgaagtcg tcatcggcga ccgagcgcac
     1981 ctcgccgctg ccgtccagaa aggtcacgtg ccatctcccg tccacaatga cccaggtgga
     2041 cggcggaaag cgcttgatgc cggtaactgc tttcgccgtc ccagcggccc gccgaaccgc
     2101 ggcaagcgcg gcctgctggc ggtgctggtc cgtcacatcg gcctggcaca ggcccaccgc
     2161 caggcacacc agcaccattg cctcgagcac ccgttggttc atgtctgcac ctcccaaagg
     2221 ccacggtggc cgccgccact gatccagacg ttgtacttgc cgtggatatc ccactcgggg
     2281 tcagtgtcct tcatgtgctt gatggcctcg ttgacggtca tcccccagtc gcacagacaa
     2341 tgcatgaact cgatccagcc cgtctcatcc gaatcgatct cgccagcgaa agcgacgacg
     2401 cagagggccc cgctgtacga cagggccggt ccgaacgtct gtggccgaca ggcagcactt
     2461 gaggaacacg aacctggcct gagatggggc tgtctgcgct cgatccagga catcctgtgc
     2521 ggtagtcggg tcgctgtggc ggttgaagat cgcgtcgaag gtcccgtggc cgttgaagac
     2581 cacgacatca tccgtgaaca gcgcctcgag tgtctccgct ttggtcggct cgacttgata
     2641 gccgccacgg gcaaccggcc cctgtgggcc atactcgtac cgagtgcaga agaactcagc
     2701 atcactcggc tgctgactgg gcgcatccac cagtcctagg gcctcgtacc actcctgagc
     2761 ccgggcccgt gcgtcgttgt agggtgcccc gccctggttg tcccccgcga tgcacgtcgc
     2821 cgacggtata ccgaacgaca ccagagccgg ctccaccggg tgctcgacgt tgccggcgta
     2881 cttggacacc cctccatggc catcgtagcc ggtcacggcg acccagtagg tgccggccgc
     2941 ctcgagcgga cccacgttgg gcacgtagtc cgtgtgctcc ccgggcgagc agaccagcgc
     3001 cgagctcgcc accggctcga tcgggacggt ccgtaccacc gtggcagcga gcgtcgaggg
     3061 aggagtcgcg tccgtcacga cgttcgtgtg cgccacc
//
LOCUS       DAOSPL010000429         3096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31566_length_3096_cov_2.845774, whole genome shotgun sequence.
ACCESSION   DAOSPL010000429 DAOSPL010000000
VERSION     DAOSPL010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31566_length_3096_cov_2.845774"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PLQ54_08840"
     CDS             <1..567
                     /locus_tag="PLQ54_08840"
                     /inference="COORDINATES: protein motif:HMM:NF036831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOS93405.1"
                     /translation="PEAQLDRFMFKILVRFPSRDELGYIIGRTTGTYDQPIQKVTGPE
                     RLNEMRLLVREVPIAEYVRDYTVRLVLATHPGSPFAPPRVTQFVSWGSSPRGAQALIL
                     AGKTRALLQGRFNVSFEDIRAVVHSALRHRMILNFEGEAESIQTDSLIDDILAHVPEA
                     AEPPKPADLPAAPPAAPAASEAEAASTR"
     gene            580..1467
                     /locus_tag="PLQ54_08845"
     CDS             580..1467
                     /locus_tag="PLQ54_08845"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOS93406.1"
                     /translation="MAEPLLDPGFLSQLERLCLLTRKVFRGTLKGEKRSRKQGTSIEF
                     ADYRDYVPGDDFRRIDWCLYGRLDRLFIKLFVEEEDLNVYLLIDQSESMSFGSPAKFR
                     YARQVASALAYVALCNLDRVMVAAFSWQLDATFGPVRGRASVFRLLEHLERLEPGGAT
                     RLSACMKAAALRMKRPGLLVVLSDFLDPSSYGPGLSALLGRGFDVGVIQVLDRTELVP
                     DLVGDLRLVDSETGDAREVTVGGGLLRRYQRTVDAYCGELRAWCMSHGASYVLASTAT
                     PVSDLVLRSLRQQRLIAQR"
     gene            1563..2300
                     /locus_tag="PLQ54_08850"
     CDS             1563..2300
                     /locus_tag="PLQ54_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93407.1"
                     /translation="MRVGGIGSYPQLTTIAAWRQAMAEPAAHGMMAFDGHGMTEHCTE
                     GLSVAHVSAHERGHLNSYRMRALAEGYEVVSEDIDIRFEMRDGRLVAVAGEAKARLEP
                     RESHQGTDTAAAHGGATAPATQAASGEASAAEAEGESRDGGTVPSASAEHELKRERQE
                     AAGQVRSAEAALSSLPATPRTEDERRRIADAQTDKVEGEERLRSADLALQAEQLARMI
                     AQQSAVISGAVEAQTQLAARIAYTARA"
     gene            2412..>3096
                     /locus_tag="PLQ54_08855"
     CDS             2412..>3096
                     /locus_tag="PLQ54_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOS93408.1"
                     /translation="MPRTHTFLVACMAAALAPCARADVTLPHVFGSHMVLQRDQAIPV
                     WGWADAGEEVAVKLGDLPVATATADATGAWRVDLPAQPAGGPLTLTVTGANTLTLDDI
                     LIGDVWLCSGQSNMEWTVNGCTDAELARVEANYPGIRHIQVPKVPAGTPARDFEGAWQ
                     VCSPETVGGFTAVGYFFARHLHRELNVPIGLINTSWGGTPAESWASEAALRREPSAGY
                     AFERWDRWQR"
BASE COUNT          479 a         1034 c         1073 g          510 t
ORIGIN      
        1 cccgaggccc agttggaccg cttcatgttc aagatcctgg tgaggttccc cagccgcgat
       61 gagctggggt acatcatcgg ccgcaccacg ggcacctacg accagccgat ccagaaggtc
      121 acgggccccg agcggctcaa cgagatgcga ttgttggtga gggaggtacc catcgccgag
      181 tacgtgcgcg attacacggt gcgcttggtg ctggccacgc acccgggttc gcccttcgcc
      241 ccgccacggg tcacccagtt cgtttcgtgg ggctcctcgc cccgcggcgc ccaggcgctt
      301 atcctggctg ggaagacccg ggcgctgctg caggggcggt tcaacgtctc cttcgaggac
      361 atccgggccg tggtccattc agccctacgc caccggatga tcctgaactt tgagggtgag
      421 gccgagagca tccagaccga ttcgctcatc gatgacatcc tggcccacgt gcccgaggcg
      481 gcagaaccgc cgaagccagc cgatctcccc gcggcgccgc ccgctgctcc ggctgcgtct
      541 gaggccgaag cggcgtcgac ccgatagcgg ggacggagca tggccgagcc tcttctcgac
      601 cctggctttc tgtctcaact cgagcgcctc tgtctgctca cccgcaaggt gttccgcggg
      661 acgctgaagg gtgagaagcg gagcaggaag caaggcacgt ccattgagtt cgccgactac
      721 cgggattacg tgcccggcga cgacttccgg cggatcgatt ggtgcctcta cggccggctg
      781 gaccggctgt tcatcaagct cttcgttgag gaagaggatc tcaacgtcta cctgctcatc
      841 gaccagagcg agtcgatgag tttcggcagc cccgccaagt tccgctacgc caggcaggtg
      901 gcttcggcgc tggcctacgt ggccctgtgc aacctcgatc gggtcatggt ggccgcgttc
      961 tcgtggcagc tggatgccac attcggcccg gtccggggca gagcgtccgt gttccggctt
     1021 ctcgagcacc tggagcgcct ggaaccgggt ggcgcgacgc gcctctcggc ctgcatgaag
     1081 gccgccgcgc tgcggatgaa gcgtccgggg ctgctcgttg ttctttcgga cttcctggac
     1141 ccctccagct atggcccggg gctctcagcc cttctcgggc gcgggttcga tgtgggtgtc
     1201 atccaggtgc tcgatcgcac cgagctggtg ccggacctgg tgggtgacct ccggctggtc
     1261 gattccgaga cgggcgacgc cagggaggtc acggtcggcg gcgggttact gcgacgttac
     1321 caacggacgg tcgacgccta ctgcggcgag cttcgtgcat ggtgcatgag ccacggcgcg
     1381 agctacgtgt tggcctccac tgccacaccc gtcagcgatt tggtgctccg ctcactgagg
     1441 cagcagcgac tcatcgcaca gcggtgagcg cgggttctgc gggggtgggg atgatcgccc
     1501 tcaagttccg cgccccctct gccgacaacc gatgtagtag aggtgtgcgc gaggtgtgac
     1561 cgttgcgcgt tggtgggatc ggctcctacc ctcagttgac gacgatcgcg gcgtggcggc
     1621 aggccatggc tgagccggcc gcgcacggga tgatggcctt cgacggccac gggatgaccg
     1681 agcactgcac tgagggtctg tcggtcgctc acgtctctgc tcacgaacgc ggtcatctca
     1741 acagctatcg gatgcgggca ctggccgagg gctacgaggt cgtctccgag gacatcgaca
     1801 tccggttcga gatgcgcgat gggcggcttg tggcggtggc gggtgaggcg aaggccaggc
     1861 tcgagccgag ggagagtcac caggggactg acaccgccgc cgcgcatggt ggcgccaccg
     1921 caccggcaac gcaggccgcg agcggcgagg cctccgccgc ggaggccgag ggcgagagcc
     1981 gtgacggcgg caccgtgccc tccgcgtcgg cggagcacga gctcaagcgc gaacggcagg
     2041 aggccgccgg tcaggtgcgg tccgccgagg cggcgctctc gtcgttgcca gccacaccgc
     2101 gcacagaaga tgagcggcgc cgaatcgcgg acgcgcagac agacaaggtc gagggcgaag
     2161 agcgcctccg gtcagccgac ctggccctcc aagccgagca gctcgcccgc atgatcgctc
     2221 agcagagcgc ggtcatttca ggcgcggttg aggcccagac ccagctcgcc gcccggatcg
     2281 cctacacggc gagggcgtag atgccctcgg aggcaaccgt ctctcctcgc cgcagcgacg
     2341 cttgaggtag actgtgccca agggccgcgc ctgccgctcc ccgcattcgt ttcatgagag
     2401 aggccaacat tatgccccga acccacacgt tccttgtcgc ctgcatggcg gcagccctgg
     2461 cgccctgcgc ccgggccgat gtcactctac cgcatgtgtt cggcagccac atggtgctgc
     2521 agcgcgacca ggccatcccg gtctggggat gggccgacgc cggcgaggag gtcgcggtga
     2581 agctcggcga cctgcccgtc gctacggcca cggccgacgc cactggcgcc tggcgcgtcg
     2641 acctgccggc ccagcccgcc ggcgggccgc tgaccctcac cgtcaccggc gcgaacacgc
     2701 tcacactgga cgacatcctg atcggcgatg tctggctttg ctcgggccag tcgaacatgg
     2761 agtggaccgt gaacgggtgt accgacgccg agttagcgag ggtggaggcc aactaccccg
     2821 gcattcgtca catccaggtg cccaaggtcc cggcggggac tcccgcgaga gacttcgagg
     2881 gcgcctggca ggtctgcagc ccggagacgg tgggcgggtt cactgctgtc ggctacttct
     2941 tcgcgaggca cctgcaccgc gagctcaatg tgcccatcgg cctgatcaac acttcctggg
     3001 gcgggacgcc ggcggagtcc tgggcgagcg aggcggcgct tcggcgcgaa ccgtctgccg
     3061 ggtatgcctt cgagcgctgg gataggtggc agcgcg
//
LOCUS       DAOSPL010000430         3093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31602_length_3093_cov_3.025675, whole genome shotgun sequence.
ACCESSION   DAOSPL010000430 DAOSPL010000000
VERSION     DAOSPL010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31602_length_3093_cov_3.025675"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PLQ54_08860"
     CDS             complement(<1..793)
                     /locus_tag="PLQ54_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93409.1"
                     /translation="MMVRALCAFELVMVLAPAGASVYNLHVVTDASPDYSSLDDFIGS
                     ATTRWDTPERKLWALFYWNHIARRQCAPMVLHGIALTDPIRQFNDYGFTMCSTVAGIN
                     CSIWDAMGLGVRYWDISNHTVPEVEYGGRWHMYDSSLSALYTLCDGETIAGVEDIGAT
                     HGCEASGGRQEPGHIAKYHCLTATSPNGFLTGSDTTRSLDDEYRCFNPNGLMYRSYFN
                     DWDRGHRYILNLRDGESYSRFYQKQGDTPAYYVPLDGKDPDDGRFG"
     gene            complement(916..2148)
                     /gene="rhaI"
                     /locus_tag="PLQ54_08865"
     CDS             complement(916..2148)
                     /gene="rhaI"
                     /locus_tag="PLQ54_08865"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOS93410.1"
                     /translation="MDQLDMQCYAALEERLAAKGIDVEGVKRRLKAQEIETPSWGYAD
                     SGTRFGKTCQPGAAVTIQEKIEDAAEVHKLTGVAPRVAVHVLWDFPDGLDSVGDVAGY
                     AASLGIGIGAINPNVFQDQAYRYGSITHHDAAVRQRAVDHMLDSIEIGKALDSKHLSL
                     WFADGTDYPGQADFRRRKHWMESCLKTVHDAMPAGMTMLVEYKLFEPGFYHTDLADWG
                     MSYVVSKKCGPQAKVLVDLGHHAHGVNIEHIVAFLLDEGMLGGFHFNNRKSADDDLTV
                     GSINPYEFFLIYNELVAAEDDPAIDCPVAYCVDQSHIMKPKLEAMIQTIVMCQTTLAK
                     ALIVDRKALQAAQSVGDIVSAENVLVEAFNTDVSPLLRRVREEMDLPPDPLAALRASG
                     YQERIAAARGVRAGAGLG"
     gene            complement(2178..>3093)
                     /locus_tag="PLQ54_08870"
     CDS             complement(2178..>3093)
                     /locus_tag="PLQ54_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93411.1"
                     /translation="RALRGRRIGILGHTFDGMGDLYVDASTLLATTGVEVTHLSFAAL
                     ARHLGAVPAEAVRVSMERDRQEFAIDPDVADEDHERSARLELAIRALIAEERLSALCA
                     LFTTATDHPAIRTQPFLAVSKLLAEGFGYGGEGDPTSATAVMLMQQLTKETNFAEMFT
                     IDFAGGGVFMNHMAETNYRLGRPDMPVRLIRNAAAFLPGEPSVCAFTVQKPGPVTLMN
                     LTAAAGGRLKMIGMCGEVADWGPAPHVRTPHFMFRPRSADGVEAFLTRYSLEGGSHHL
                     ALGYGDQRGPLAAAARMMGIEWVEVTDL"
BASE COUNT          524 a         1050 c          979 g          540 t
ORIGIN      
        1 gcccaaagcg cccgtcgtcc ggatccttcc cgtcaagcgg cacgtagtag gccggagtat
       61 cgccctgctt ctggtagaag cgcgagtagc tctctccgtc gcggaggttg aggatgtacc
      121 tatgcccgcg gtcccagtcg ttgaagtagg agcggtacat cagcccgttt gggttgaagc
      181 accggtactc gtcatccaga ctgcgggtgg tgtcggagcc cgtgaggaaa ccgttcggac
      241 tcgtggccgt caggcagtgg tacttggcga tgtggcccgg ctcctggcgt ccgccggaag
      301 cctcgcagcc gtgcgtggcg ccgatgtcct ccacgcctgc gatggtctcg ccatcgcaca
      361 gcgtgtagag ggccgaaagg ctgctgtcat acatgtgcca gcgcccgccg tactcgactt
      421 cgggcaccgt gtggtttgat atgtcccagt agcggacgcc gagccccatg gcgtcccaga
      481 tcgagcagtt gatgcccgcg accgtgctgc acatggtgaa gccatagtcg ttgaactgcc
      541 ggatcgggtc ggtcagggcg atgccgtgaa gcaccatagg cgcgcactgc cggcgcgcga
      601 tatggttcca gtagaacagc gcccacagct ttcgctcagg cgtgtcccat cgggtggtag
      661 cgctgcctat gaaatcgtcg agggatgagt aatcggggga ggcgtcggtc accacgtgga
      721 ggttatagac cgaggcgcca gccggcgcga ggaccatcac caactcaaac gcacagagag
      781 cacgcaccat catgcatccc ccctgccaat gccgaccggg gaccgactgg agtctgcggc
      841 acctgtcgca gatccgatcg tgtccccggt ttgcgcgttc catcccatcg cgggccacct
      901 acccgcgagg gagtcctaac ccagccccgc ccccgcgcgc acgccgcggg ccgcggcgat
      961 ccgctcctgg tacccgctgg cgcgcagggc cgccaacgga tcaggcggca ggtccatctc
     1021 ctcgcggact ctcctcagca gcgggctcac atcggtgttg aaggcctcga ccagaacgtt
     1081 ctcggccgac acgatgtcgc ccacgctctg ggcggcctgg agcgccttcc gatccacgat
     1141 cagggccttt gccagtgtgg tctggcacat gacgatggtc tggatcatgg cctcgagttt
     1201 cggcttcatg atgtgcgact ggtccacgca gtaggcgacg gggcagtcga tggcggggtc
     1261 atcctcggct gccacgagct cgttgtagat caggaagaac tcgtaggggt tgatcgaacc
     1321 caccgtcagg tcatcatcgg ccgacttccg gttgttgaag tggaacccgc ccaacatgcc
     1381 ctcgtccagc aggaacgcca cgatgtgctc gatgttgacc ccatgggcat ggtgacccaa
     1441 gtccaccagc accttagcct gggggccgca cttcttcgag accacatagg acatgcccca
     1501 gtccgccaag tcggtgtggt agaacccggg ctcaaacagc ttgtactcga ccaacatggt
     1561 catgcccgcg ggcatggcat cgtgaacggt cttcaggcag ctctccatcc agtgcttgcg
     1621 gcgccggaag tccgcttggc ccggatagtc tgtgccgtcg gcaaaccaca ggctcaggtg
     1681 cttcgagtcc agcgccttgc cgatctcaat gctgtccagc atgtgatcca ccgcccgctg
     1741 ccggacggcc gcatcgtggt gggtgatgct cccgtagcgg taggcctggt cctggaacac
     1801 attcgggttg atggcgccga tcccgatccc taacgatgcc gcatagcccg cgacatcgcc
     1861 gaccgagtcc aggccgtccg ggaaatccca cagcacgtgc acggccacac gcggggccac
     1921 gcccgtcagc ttgtggactt ccgccgcgtc ctcaatcttc tcctggatcg tcaccgccgc
     1981 gcccggttgg cacgtcttgc caaagcgcgt gcccgagtcg gcgtagcccc acgagggggt
     2041 ctcgatctcc tgcgccttca gccggcgctt cacgccctca acgtcaatcc ctttcgccgc
     2101 cagccgctcc tcgagcgcgg cgtagcactg catgtcgagc tggtccatcc gtggagcctc
     2161 ctccagactg gttcgggtca gaggtccgtg acctcgaccc actcgatgcc catcatcctc
     2221 gcggcggccg ccagcggacc ccgctgatca ccgtacccca acgccaggtg gtgtgacccg
     2281 ccctcgaggc tgtagcgtgt gaggaaggcc tcgacgccat ccgctgaacg agggcgaaac
     2341 atgaagtgcg gcgtgcgaac atgcggcgcc ggcccccagt cggccacctc gccgcacatg
     2401 ccgatcatct tgaggcggcc gcccgccgcg gccgtaaggt tcatcagcgt caccggtccc
     2461 ggtttctgga ccgtgaaggc gcacaccgac ggctcgcccg gcaggaatgc cgctgcgttc
     2521 cgtatcaggc gcaccggcat gtccggccgg ccgagacggt agttcgtctc ggccatgtgg
     2581 ttcatgaaga ccccgccgcc cgcgaagtcg atggtgaaca tctcggcgaa attggtctcc
     2641 ttggtgagct gctgcatgag catcaccgcg gtggccgagg taggatcgcc ctcgccgccg
     2701 tacccaaagc cctccgcgag gagcttcgag accgccagga acggctgcgt gcggatcgcc
     2761 gggtggtcgg tcgccgtggt gaagagcgcg cacagggccg atagacgctc ctcggcgatg
     2821 agcgcacgaa tggccagctc gaggcgcgcc gatcgctcgt gatcctcgtc ggctacgtcc
     2881 ggatcgatgg cgaactcctg ccggtcgcgc tccatcgaga cccgaaccgc ctcggctggc
     2941 accgcgccca gatggcgcgc cagggcggcg aacgagaggt gcgtgacctc aacacccgtt
     3001 gtggccagaa gtgtcgaggc atccacatag aggtcgccca tgccatcgaa cgtgtggccc
     3061 agaatcccga tgcgccgccc acggagtgcc cgg
//
LOCUS       DAOSPL010000431         3090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31629_length_3090_cov_4.519605, whole genome shotgun sequence.
ACCESSION   DAOSPL010000431 DAOSPL010000000
VERSION     DAOSPL010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31629_length_3090_cov_4.519605"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLQ54_08875"
     CDS             complement(<1..509)
                     /locus_tag="PLQ54_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOS93412.1"
                     /translation="MSRYILSLDQGTTSSRAIILDHQGAIVAQAQREITQIYPQPGWV
                     EHDPREILGTQRVAAREALARAGLAAAEIIAIGITNQRETTIVWDRHTGEPVCNAIVW
                     QCRRTAPLCEELARQGAADLIRARTGLVIDPYFSATKVRWILEHVPDAERRAQAGDLL
                     FGTVDSWLI"
     gene            607..1371
                     /locus_tag="PLQ54_08880"
     CDS             607..1371
                     /locus_tag="PLQ54_08880"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HOS93413.1"
                     /translation="MDQRDAAARIAEGLIGAGVRSGGTVLVHSSLKSLGRVPGGPETV
                     ILGLLEAIGPEGTLLLPALSYATVHAANPVFDVRRTPSCVGAIPEYFRTRPGTLRSVS
                     PTHSVCGVGPRAEELLAKQQLDDTPCGPHSAFRALPDIGGQIVMLGCGLAPNTSMHAV
                     EEVAEAPYLFGATVEYRVVLFDGRETRLRVRRHAFAGWRQRYERLAWILPPEALRAGP
                     VLEATAHVIEAAVMWPMAVETIGREPYYFVEREERS"
     gene            1371..2624
                     /locus_tag="PLQ54_08885"
     CDS             1371..2624
                     /locus_tag="PLQ54_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93414.1"
                     /translation="MISRSDRNRLRVVAQRYAEMTMSAAMDARRHVWRRTNQLEERTV
                     PFQIEDNGTFFADLTPPPECEGEFERGLEYGLLRALTNHELIDDDRVFPPFCGINWVI
                     DRTSICPELGVRRAPDATGRDLGYETNTPLADLANSFHKLRPTECSVDRAETQRRAEM
                     AQDAFGDILPVRIVGHDVTFAGTGLVGRAVHLVGMDDLYIAMVDQPETVHRLLEFITQ
                     DAERFIDWLEAERLITPNSHEMDVGSGSCGLTDELPRSDRAQGEPLGAMDCWVWIEAQ
                     EAVGLSPAMYADFIYPYQKRLADRFGLVYYGCCEAVHAFWPTLRGMTNIRKVTMSPWC
                     DQHSLAAAVGDTVVLSRKPHPMKLCGARFDAAEFEAHIRETLGAAEGRFVELIFRDTC
                     TLDGTMKDRVAEACRIVRRLIGRGG"
     gene            2804..>3090
                     /locus_tag="PLQ54_08890"
     CDS             2804..>3090
                     /locus_tag="PLQ54_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93415.1"
                     /translation="MQIALTTLAAMAAATQVAGAVAATPQELAASHAWVAEKILGEGV
                     AEGPAGLIVLANNDPVQPNERNGGKLRIVDQVFDRGLFCHAVSHVVVRLPG"
BASE COUNT          487 a          972 c         1145 g          486 t
ORIGIN      
        1 cagatgagcc atgagtccac ggtgccaaac agcaggtcgc ccgcttgggc cctccgctca
       61 gcatcgggaa cgtgttcgag gatccaccgc actttggttg ccgagaagta gggatcgatg
      121 acgaggccgg tgcgagcgcg gatgaggtcg gcggcgccct gccgggcgag ttcctcgcac
      181 agcggcgcag ttcgccggca ctgccagacg atggcgttgc acacgggctc gccggtgtgg
      241 cgatcccata cgatcgtggt ctcacgctgg ttggtgatcc cgatggcgat gatctcggca
      301 gcggccaggc cagcccgagc cagggcctcg cgcgcagcca cgcgctgggt gccgaggatc
      361 tcgcgcgggt cgtgttccac ccatccaggc tgagggtaga tttgggtgat ctcgcgctgg
      421 gcctgggcga cgatggcgcc ctgatgatcg aggatgatgg cgcgggagct ggtggtgccc
      481 tgatcgagtg acaggatgta ccgggacaac ggcgaagccc cttccccgcg gtctgcggtg
      541 cgtgatacgg gattccggac atgggtcgcc gaggccttcg ggcatcggac ggggaggtcg
      601 tgctggatgg atcagcgaga cgcggcagca cgcattgccg aggggcttat cggcgcggga
      661 gtccggagtg gcgggacggt actggtccac tcgtcgctca agtcgttggg gcgggtgccc
      721 ggtgggcccg agacggtgat cctcgggctc ctcgaggcga taggaccgga aggcacgctg
      781 ctcctgccgg ccctcagtta cgcgacggtc cacgcggcca acccggtgtt cgacgtgcgg
      841 aggacgccct cgtgcgttgg ggcgatcccc gagtacttcc gcacgcggcc cgggacgctc
      901 cgcagcgtga gcccaacgca ctcggtgtgc ggcgtggggc cacgggccga ggagctgctc
      961 gcgaagcagc agctcgacga cacgccgtgt gggccacatt cggcattccg ggcgcttccg
     1021 gacatcgggg ggcagatcgt catgctgggc tgcggcctgg caccgaacac gtcgatgcac
     1081 gccgtcgagg aggtggccga ggctccgtat ctgttcgggg caacagttga gtaccgcgtg
     1141 gtcctgtttg acggccgcga aacacgcctg cgcgttcgtc gccatgcgtt tgcgggctgg
     1201 cggcagcggt acgagaggct ggcgtggatt ctgccccccg aagcactgcg cgcggggccg
     1261 gtgctcgaag cgacggccca tgtgatcgag gcggcagtca tgtggccgat ggctgtggag
     1321 accatcgggc gggaaccgta ctatttcgtc gagcgcgagg agcgaagttg atgatatcgc
     1381 ggagcgaccg gaaccgcttg cgtgtcgtgg cgcagcgata tgcggagatg accatgagtg
     1441 ccgccatgga cgcgcggcgt catgtctggc gcaggacgaa ccagctcgag gagcgcaccg
     1501 tcccgttcca gatcgaggac aacggcacgt tcttcgccga cctgacgccc ccgcctgagt
     1561 gcgagggcga gttcgagcgg gggctcgagt atgggctgct ccgagcactg acgaaccacg
     1621 agctcatcga cgatgaccgc gtctttcccc ccttctgcgg catcaactgg gtcatcgacc
     1681 gcacatcgat ctgcccggag ctgggcgttc ggcgggcgcc cgacgccacg gggcgggatc
     1741 ttgggtatga gacgaacacg cccctcgccg acctggcgaa cagcttccac aagctgcggc
     1801 cgacggagtg ctccgtcgac cgggcggaga cccagcgtcg ggctgagatg gcgcaggacg
     1861 ccttcggcga cattctgccc gtgcggatcg ttggacacga tgtgaccttc gcgggaaccg
     1921 gcctggtggg gcgcgccgtg catctggttg gcatggacga cctctacatc gccatggtgg
     1981 accagcccga gaccgtgcac cggctgctgg agttcatcac ccaggacgcg gagcggttca
     2041 tcgactggct cgaggctgag cggctgatca cgccgaacag ccacgagatg gacgtgggct
     2101 cggggagttg cgggctcacc gacgaactgc cgaggtcgga ccgcgcacag ggggaaccgc
     2161 tcggcgcgat ggactgctgg gtgtggatcg aggcgcagga ggccgtgggg ctctcgcccg
     2221 cgatgtacgc ggacttcatc tatccctatc agaagcgcct cgcggatcgg ttcggcctcg
     2281 tctactacgg ctgctgcgag gcggtacacg ctttctggcc gacgctgcgc ggaatgacaa
     2341 acatccgcaa ggtgacgatg tcgccctggt gcgaccagca ctcactggcc gcagcggtcg
     2401 gcgacacggt ggtgctctcg cgcaagccgc acccgatgaa gctgtgcggg gcgcggttcg
     2461 acgcggcgga gttcgaagcg cacatccggg agacgctcgg ggcggccgag ggccggttcg
     2521 tcgagctcat cttccgcgac acgtgcacgc tcgacggcac gatgaaggac cgcgtggccg
     2581 aggcgtgccg gatcgtgcgg cggctcatcg ggcggggtgg gtaggcgttg agcggagccg
     2641 gccgagtcgg cggagccagc gggggtagcc aggctccccg ggcgggccgc ctgggccacc
     2701 ggcacggagt ccggtgccgc tcgagggttg cgctggaggc ggcgacgcgc gcgcgggcga
     2761 acctcgctag tgaacccgac acacacaccg cgcgaggtga accatgcaga ttgcactgac
     2821 cacactggcc gcgatggccg ccgcaacaca ggtggccgga gctgtcgcag ccacccctca
     2881 ggagctcgcc gcctcccacg cttgggtggc tgagaagatc ctcggcgagg gggttgccga
     2941 gggcccggcc gggctcatcg tgctggccaa caacgacccc gttcagccga acgagcgcaa
     3001 cggcgggaag ctgcgcatcg tcgaccaggt cttcgaccgt ggcctgttct gccatgcggt
     3061 gagccacgtc gttgtgcggc tgcccggcca
//
LOCUS       DAOSPL010000432         3089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31655_length_3089_cov_2.719842, whole genome shotgun sequence.
ACCESSION   DAOSPL010000432 DAOSPL010000000
VERSION     DAOSPL010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31655_length_3089_cov_2.719842"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="PLQ54_08895"
     CDS             complement(<1..939)
                     /locus_tag="PLQ54_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93416.1"
                     /translation="MWRHARGVCLLVLTLALVSAGCGPGTVTAGDLTFQQRSDAIDAV
                     EAHAASLPGGDHAATLASVAAYMRSRPEFAESGVSEEYGCTWARFVDGRILVFRDDPP
                     PTAGESIVGDRGCTGTLRTGQPVGKARVLQCLSGYVDATPWLRDWLRSAGYDVSADLE
                     NPTVERLRSVQGQDGVFFISTHGGGGVAERGQGPLFCLMTTTPVTDENDRRYESDLKD
                     HSLMILDCLGTPTTRVRRYAITAKFVRDHMRFGPNSLVYVDACYSNDREFREACFDAG
                     ASLYAGWTGAVLDWVSARAALFMFDRMLGANATTASE"
     gene            1135..2181
                     /locus_tag="PLQ54_08900"
     CDS             1135..2181
                     /locus_tag="PLQ54_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS93417.1"
                     /translation="MSRVPDNDFDRLRMALTLTGKPDRVPLMELDVSRAVMGAYLGRP
                     IRGIEDIVEFHVQAGYDFAPTQVGLMRVGSPIATEATTVKSAHYGVYSDEPTAREWAA
                     EGRGVVATHEDIDAFAWDHGHALDLTVLDQAAAVLPESMQLFAIVGKVFTAVWMLMGF
                     EGFAFALKENPGVVGRLFRIVGEIQLEVVRRAAAHPAVAVLVHPDDIAYTEGLMVHPD
                     ILREHVFPWYQRMGEVCRAHGKIMIYHSDGDLWPVFEDIIACGFSAVHPLEPKAMDAR
                     EVKSAYGDRLCLLGGVELDRLSRGTPGEIRHMSSEMIRDLAYDGGYCLGSSNSVTDYV
                     PLEIYRAMIETVWL"
     gene            complement(2277..>3089)
                     /locus_tag="PLQ54_08905"
     CDS             complement(2277..>3089)
                     /locus_tag="PLQ54_08905"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOS93418.1"
                     /translation="PAVQVNGVGNRVTHNLINDGPHMAIMWGGNDHVFEYNEIHSVVH
                     SANDAGIMYAGYDPAMRGHMIRHNYFHHVYGFQNRGCNGVYLDDMFCSATIYGNVFYK
                     VPRAAFIGGGHDNVVENNIFVDCNPALHVDARMMNWAAASVPIMRERLEAVPYREEPW
                     RSRYPQLLTYLDGNYAQPRGNLVARNICWGGSWDEIEESARPGVELVDNLVGVDPLFV
                     DAEGGDFRLREDSPAWKLGFQRIPTERIGLYESPLRASWPVVTTVRPPWEGE"
BASE COUNT          509 a         1088 c          973 g          519 t
ORIGIN      
        1 ctcggacgcc gtcgtggcgt tcgcgccaag catgcggtcg aacatgaaga gcgccgcccg
       61 cgccgacacc cagtcgagta ccgctccggt ccagcccgcg taaagcgaag ctccggcgtc
      121 gaagcaggcc tctcggaact cccggtcgtt gctgtagcac gcgtcaacgt agaccaggct
      181 gttcgggccg aacctcatgt ggtcccggac gaacttggcc gtgatggcat accgccgcac
      241 ccgagtggtg ggagtcccga ggcagtcgag aatcatcaga gagtggtcct tcagatccga
      301 ctcgtaacgc cggtcattct catcggtgac gggcgtggtc gtcatgaggc agaacagcgg
      361 gccctggccg cgttcggcca cgcctccccc gccatgggtg ctgatgaaga agacgccgtc
      421 ctggccctgg acgcttcgca gcctctctac ggtggggttc tcgagatcgg cactcacatc
      481 gtacccggcc gacctcaacc agtcgcggag ccacggcgtt gcatcgacgt agcccgagag
      541 gcactggagc actcgagcct tgcccacggg ctgccccgtg cgcagcgtgc ccgtgcaccc
      601 tcggtccccg acaatggact caccggcagt tgggggcgga tcgtcgcgga agaccaggat
      661 ccggccgtcc acgaatcgtg cccacgtgca gccatactcc tcggatacgc ccgactccgc
      721 gaactccgga cgggatctca tgtacgcggc caccgacgcc agcgtggctg catggtcccc
      781 tcccggcagc gaagccgcat gcgcctcaac ggcgtcgatg gcgtcggacc gctgctggaa
      841 agtcaagtcc cccgccgtga ccgtccccgg cccgcatcct gccgagacca gagcgagagt
      901 caggaccagc aggcatacgc cgcgcgcgtg gcgccacatg accagccccc ccgggtcccc
      961 ggcgaagacc ccgcgggcgc gatgcccgcg gccctccaac ggtttcggcg cagccgagcc
     1021 ggtgtccttt gggatccgtt ggtcgcgccg gcctcgccgt gctctccgag gagcgccggc
     1081 ctggcgagtc gaagactccg gcggcacctc ttcctcacgc caaggaggcc cgagatgtca
     1141 cgggttcccg acaacgactt cgaccgcctg cgaatggccc tcacactcac cggcaagccg
     1201 gaccgcgtgc cgctgatgga gctcgacgtc agccgcgcgg tcatgggagc ctacctcggc
     1261 cgtcccattc ggggcatcga agacatcgtc gagttccacg tgcaggccgg gtacgacttc
     1321 gcgcccaccc aggtgggcct catgcgtgtg gggagcccga tcgccaccga ggccaccacc
     1381 gtgaagtcgg cgcactatgg cgtctactcc gacgagccca ccgctcgcga gtgggcagcc
     1441 gagggccgcg gtgtcgtcgc cacgcacgag gacatcgatg ccttcgcctg ggatcacggc
     1501 cacgcactgg acctgacggt cctcgaccag gccgcagccg tccttcccga gtccatgcag
     1561 ctcttcgcca tagtcggcaa ggtcttcact gcggtgtgga tgctcatggg cttcgagggg
     1621 ttcgcgttcg cactgaagga gaatcccgga gtggtcggtc gcctgtttcg aatcgtgggg
     1681 gagatccagc ttgaggtggt gcgacgcgcg gccgcgcacc cggctgttgc cgtgctggtc
     1741 cacccggatg acatcgccta taccgagggg ctcatggtcc accccgacat actccgcgag
     1801 cacgtgttcc cctggtacca gcgcatggga gaggtgtgtc gggcccacgg gaagatcatg
     1861 atctaccact ccgatggcga tctttggccg gtctttgagg acatcatcgc gtgcggcttc
     1921 agtgccgtcc atccactcga gcccaaggca atggacgccc gcgaggtgaa gtccgcctat
     1981 ggcgaccggc tgtgcctgct tggcggcgtc gagctcgatc ggctatcgcg cggcacaccc
     2041 ggggagatcc ggcacatgtc gagcgagatg atccgcgacc tggcctacga cggcggctac
     2101 tgcctgggct catcgaactc ggtgaccgac tacgtcccgc tcgagatcta ccgggcaatg
     2161 atcgagaccg tgtggcttta ggagtctccc ggcgcctgga gctcgcgggt tcctcccggc
     2221 accccttcgc aacccggccc atgggtccct ggagcctcaa gataccgcca atgcacctac
     2281 tccccctccc atggcggccg caccgtcgtc accaccggcc acgacgcccg cagggggctc
     2341 tcgtagagcc cgatccgctc cgtcgggatg cgctggaagc caagcttcca cgccggtgag
     2401 tcctcgcgca ggcgaaagtc gcccccttcg gcatcgacga acagcggatc cacgcccacc
     2461 aggttgtcga cgagctcgac gcccggcctc gcgctctcct cgatctcatc ccaactgccg
     2521 ccccagcaga tgttccgcgc caccaggttc ccgcgcggct gggcgtagtt gccatccaga
     2581 taggtcagga gctgcggata gcggctccgc cagggctcct cgcggtacgg cacggcctcg
     2641 aggcgctccc tcatgatcgg caccgacgcc gcggcccagt tcatcatgcg ggcatcgacg
     2701 tgcagcgccg ggttacagtc gacgaagatg ttgttctcga ccacgttgtc atgcccgccc
     2761 ccgatgaacg cagcccgcgg caccttgtag aacacgttcc cgtagatggt ggccgagcag
     2821 aacatgtcat cgaggtagac gccgttgcac cccctgttct ggaagccata gacgtgatgg
     2881 aagtagttgt ggcggatcat gtgcccgcgc atggccgggt cgtagcccgc atacatgatg
     2941 cccgcatcat tggcactgtg gaccacgctg tggatctcgt tgtactcgaa cacgtgatcg
     3001 ttcccgcccc acatgatggc catgtgcggg ccatcgttga tcaggttgtg cgtcacgcgg
     3061 ttgcccacgc cgttcacctg cacggcggg
//
LOCUS       DAOSPL010000433         3086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31686_length_3086_cov_3.184758, whole genome shotgun sequence.
ACCESSION   DAOSPL010000433 DAOSPL010000000
VERSION     DAOSPL010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31686_length_3086_cov_3.184758"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="PLQ54_08910"
     CDS             <1..925
                     /locus_tag="PLQ54_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOS93419.1"
                     /translation="FAFASPWFIGFLLFTGGPIVVSIVLSLCDYDVLHAPRYVGLDNY
                     RALLSVERVEMPAEEGKPARTRLQANEPTFWKSLFNTTFMIIGVPISMAAGLAIAMLL
                     NMKVGGLSVYRTIYYLPAVVPIVASSILWIWVLNPENGLLNNILRFFLPPSVAAPGWL
                     TSDKLWTGSKMALVVMGLWGAGAGMIIWLAGLKGIPQHLYEAATIDGAGPWARFWHVT
                     VPQLSPYIFFNLVMGIIGTFQIFDQAYVMTEGGPADSTLFYVYFLFKNAFQYFKMGMA
                     SAQAWILFVIVLILTLIQLKLAPRWVHYESE"
     gene            912..2627
                     /locus_tag="PLQ54_08915"
     CDS             912..2627
                     /locus_tag="PLQ54_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOS93420.1"
                     /translation="MRANDTTVTALASRPPARRVWSLLAAHLVLLAGCVVFSLPFYWL
                     LSTSVKETDEIQRDPPVWVPAWPRGAKQSPFIELRDSDEPKVPEGVDEGRFRAFWPAV
                     ESALWEWVRPRLAEEAAALPEARVRRALTGTLWNDIQAGTPKEIWSQPPAGIAKDLVA
                     GLDPEDLGKAWDRTYRFLGFRDVVVEDQGFVRHVIRLGGGQRQADLWAPASDSVTLSP
                     ARPGQKPYVAVAYDFGSEGGVSWSVDLPTEVTADQFRGVIIPVRHDHSWNRLWLTVDV
                     GDRRFAATEPLVLSDRAWLDVTWRLPDKGRTTFEVHDFQELEAQGRGEAAPGTLRLTA
                     RLERVGTLGAVWGKWSRNYRDALRYVPFWTLVANTVWIVAMCILGQVFACSLVAFSFA
                     RLNWPFRDACFGLLLATMMLPGQVTMIPQFLVFKYVGWYNTLKPLWVPAFFGSAFFIF
                     MLRQFMKGIPKDLEDAAKIDGCSYFGIYWRIMLPLIKPALAAIAIFTFMGSWNNFMGP
                     LIYVSDQRLYPLALGLFQFRSEHSADFGMLMAASALMTVPVVGIFFGAQRYFIQGVTL
                     TGMKG"
     gene            complement(2624..>3086)
                     /locus_tag="PLQ54_08920"
     CDS             complement(2624..>3086)
                     /locus_tag="PLQ54_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOS93421.1"
                     /translation="RLAEREAFVDQIVQDLRVSGHRVTPQRVAIIREFVGRADHPSAE
                     AIHQRVVAEFPMIAISTVYSTIRLLVEMGVAAEVSPGFTGARFDPNTSNHCHVVCTHC
                     GSIADVPAGPELPTAEACAETLGSGFRVERLTLDIHGVCARCAAGGSDAPD"
BASE COUNT          468 a         1047 c          989 g          582 t
ORIGIN      
        1 cttcgccttc gcctcgccct ggttcatcgg cttcctgctg ttcaccggcg ggccgatcgt
       61 ggtctccatc gtcctgagcc tgtgcgacta cgacgtgctt catgcgcccc gctacgtggg
      121 cctcgacaac taccgggcgc tcttgagcgt cgagcgcgtc gaaatgccag cagaagaggg
      181 caagccggca cggactcggc tccaagctaa cgagccgacg ttctggaaga gcctgttcaa
      241 caccacgttc atgatcatcg gcgtccccat cagcatggcg gcgggtctgg ccatagccat
      301 gctgctcaac atgaaggtgg gcgggctctc cgtctaccgg acgatctact acctgcccgc
      361 agtcgtgccg atcgtggcgt cgtcgattct gtggatctgg gtactcaatc ccgagaatgg
      421 tcttctcaac aacatcctga ggttcttcct ccccccttcc gtcgccgctc cgggctggct
      481 cacgtccgac aagctctgga caggcagcaa gatggccctg gtggtcatgg ggttgtgggg
      541 cgccggtgcc ggcatgatca tctggctcgc cggcctcaaa ggcatacccc agcatctgta
      601 cgaagcagcc actatcgatg gagcggggcc ctgggcacgc ttctggcacg tgacggtccc
      661 ccagctctcg ccctacatct tcttcaacct ggtgatgggc atcatcggaa ccttccagat
      721 cttcgaccag gcgtacgtga tgaccgaagg cggccccgcg gactcgactc tgttctacgt
      781 gtacttcctg ttcaagaacg cctttcagta ctttaagatg gggatggcct cagcccaggc
      841 gtggatcctg ttcgtcatcg tgctgatcct gacgctgatc cagctcaagc tggcgccgcg
      901 gtgggtgcac tatgagagcg aatgacacca ccgtgacggc gctcgcaagc cggccccccg
      961 cccggcgcgt gtggtcactg ctggccgcac acctggtgct gctcgccggc tgcgtcgtgt
     1021 tttcgctgcc cttctactgg ctcctgagca cgagcgtgaa ggagaccgac gagattcagc
     1081 gcgatccccc ggtctgggtg ccagcctggc cccgtggagc caagcagtcg cccttcatcg
     1141 agctgcgcga ctctgacgag cccaaggtcc ccgagggtgt cgacgagggc cgcttccgcg
     1201 cgttctggcc ggcggtcgag tccgcgctat gggagtgggt gcggccccgg ctggccgagg
     1261 aggccgcggc cctgcccgag gcacgggtgc gccgggccct gactgggacg ctatggaacg
     1321 acattcaggc cgggactccc aaggagatct ggtcgcagcc gccggccggc atcgcgaagg
     1381 acctggtggc tgggctcgac ccggaggacc tcggcaaggc gtgggaccgt acctaccgct
     1441 tcctcggctt ccgcgatgtg gtcgttgagg accagggctt cgtccgccac gtcattcgcc
     1501 tcggcggcgg ccagcgtcag gccgatctct gggcacccgc atcggacagc gtcacgctca
     1561 gccctgctcg gcccggccag aagccctacg tcgccgtggc ctacgacttc ggctccgaag
     1621 gcggcgtgtc gtggtccgtc gaccttccca ccgaggtgac ggccgaccag ttccgcggcg
     1681 tgatcatccc cgtgcggcac gaccactcgt ggaaccgact gtggctcacc gtcgatgtcg
     1741 gtgaccggcg cttcgcggcc accgagcccc tggtgctcag cgatcgggcg tggcttgatg
     1801 tcacctggcg gctgccggac aaggggagga ccacgttcga ggtccacgac ttccaggaac
     1861 tggaggccca gggcaggggc gaggccgcgc cgggcacctt gcgcctcacg gcccggctcg
     1921 agcgcgtcgg taccctcggc gccgtctggg gcaagtggtc gcgcaactac cgtgatgccc
     1981 tgcgctacgt gcccttctgg actctcgtgg ccaacacggt ctggatcgtg gccatgtgca
     2041 tactcggcca ggtgttcgcg tgctcgctgg tggcgttctc cttcgctcgc ctgaactggc
     2101 ccttccgcga cgcctgcttc gggctgttgc tggcgacgat gatgctgccc ggccaggtca
     2161 cgatgatccc gcagttcctg gtgttcaagt acgtgggctg gtacaacacc ctcaagcccc
     2221 tctgggtccc cgcgttcttt ggcagcgcct tcttcatctt tatgctgcgg cagttcatga
     2281 aggggatccc gaaggatctg gaggatgcgg ccaagatcga tggctgcagc tacttcggca
     2341 tctactggcg catcatgctg ccgctcatca agcccgctct ggcggccatc gcgatcttca
     2401 ccttcatggg gagctggaac aacttcatgg gtccgctgat ctacgtcagc gatcagcgcc
     2461 tgtacccgct ggccctcggg ctgttccagt tccgctcgga gcacagcgcc gacttcggca
     2521 tgctgatggc ggcctcggcg ctgatgaccg tcccggtggt cggcatcttc ttcggggcac
     2581 aacgctactt catccagggc gtcacgctca cggggatgaa gggctaatcg ggtgcgtcgc
     2641 tgccaccggc ggcgcaccgg gcgcagacgc cgtggatgtc cagtgtcagc cgctcgacac
     2701 ggaagcccga acccaaggtc tcggcgcacg cctcggccgt cggcagctcc gggcctgcgg
     2761 gcacatcggc gatcgagccg cagtgcgtgc agaccacgtg gcaatggttt gacgtgttcg
     2821 ggtcgaaccg agctccggtg aagccggggg agacctcggc cgcgacaccc atctcgacca
     2881 gcagacggat cgtgctgtag accgtcgaga tggcgatcat cgggaactcg gccacgaccc
     2941 tctggtggat ggcctccgcc gatgggtggt ccgcccggcc cacaaactcg cggatgatgg
     3001 ccacgcgctg cggcgtcacg cggtgcccgc tcacgcgcag gtcctgcacg atctgatcca
     3061 cgaacgcttc acgctccgcc aaccgg
//
LOCUS       DAOSPL010000434         3081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31743_length_3081_cov_3.138797, whole genome shotgun sequence.
ACCESSION   DAOSPL010000434 DAOSPL010000000
VERSION     DAOSPL010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31743_length_3081_cov_3.138797"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..949)
                     /locus_tag="PLQ54_08925"
     CDS             complement(131..949)
                     /locus_tag="PLQ54_08925"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOS93422.1"
                     /translation="MRIWDCHCHARGNETADQVLRWMDDARIDRINLFARYPGPVDGQ
                     YRRDDAHESIDHIAQIQAADPDRIYGLIWAEPRTPGMVEEIEYGIVEKGLRGIKMIPR
                     HWSPTDEMLFPIYEKMQALRKPIQFHSGILYGFEDSSRFCQPVLYEVLVNFPGLRFSL
                     AHIGWPWVDECIAVFGRFRAAVGYQTANCQMWVDTCRGTPDAWREEALRKTIAFCGSE
                     HVMFGVDATPATLVGNAPVHVAKDLALLRDIIGLSETQIDQFFWGACEAFYSPQ"
     gene            complement(950..1876)
                     /locus_tag="PLQ54_08930"
     CDS             complement(950..1876)
                     /locus_tag="PLQ54_08930"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS93423.1"
                     /translation="MTSRERVLTTIARREPDRVPYNLRPSHELLLQFRAHVGEGDFAD
                     HFGHDVRYVSLALPERPAHVPEDQWTPTPTPADIAATAVATARLHGRGLAVCGAYACG
                     VFEQAKAWLGDEGALVAPFEDPAGLARTLDRITEWKASIYGAYAEAGVDIVWMGDDLG
                     SQRSLVMSPEHYRQWYRPRHQELVEHIRWRGPSAQIAFHCCGHVTPLIPDLIEMGIDV
                     LEAVQAEAMDIAQLKREFGRDISFWGGVGAQSILARTEPDEVMAGVRDALAIMAPGGG
                     YIAAPCHTLTDEVPWASVIAFHEAVVRFGGYG"
     gene            2144..>3081
                     /locus_tag="PLQ54_08935"
     CDS             2144..>3081
                     /locus_tag="PLQ54_08935"
                     /inference="COORDINATES: protein motif:HMM:NF019219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HOS93424.1"
                     /translation="MRGALWGAVAFIAVSSAVDARAPAVAPFERPDEVVPATRIDELV
                     GASLQVAGIESAYLCSDEVFVRRAHLDIIGTLPEPLVVRAFLQDRRPEKRARLIDSLL
                     ERPEFADYWAMKWCDVLRVKAEFPINLWPNAVQCYQRWVREAMATNKPYDLFARELLT
                     SSGSAFRVPPANFYRAVQGRKPEDIAGAVALTFMGTRLASWPQTERENLARFFFRVAY
                     KKTDEWKEEIVCLDPTPLGPFDATLPDGTAVHIAADQDPRQVFADWLLADGNPWFARA
                     IVNRVWAWLLGRGIVEEPDDIRPDNPPSNPELLAYL"
BASE COUNT          487 a          930 c         1123 g          541 t
ORIGIN      
        1 ccatggggac ctccgatatg ggggcgcggc ggctcgcaac ccgcgccagg agacgtcgcc
       61 ggatcctttc gccgtgtgcc attcgccggc cttggcgtac aggcgacgcg ctcgggatgg
      121 cagcggccgg ctactggggc gagtagaacg cttcacaggc accccagaag aactggtcga
      181 tctgagtctc gctgaggccg atgatgtcgc gcaagagggc caagtccttg gccacgtgca
      241 ccggagcgtt tccgaccaag gttgccggcg tggcgtcgac tccgaacatc acgtgctccg
      301 agccgcagaa ggcgatggtc ttgcggaggg cctcttcgcg ccaagcgtcc ggcgtgccgc
      361 ggcacgtgtc cacccacatc tggcagttgg cggtctggta gccgacggcc gcgcggaagc
      421 ggccgaacac ggcgatgcac tcatcgaccc acggccaacc gatgtgggcc aaggagaacc
      481 gcaggccggg gaagttgacg agcacctcgt agagcaccgg ttggcagaag cgcgagctgt
      541 cttcgaagcc atagaggatg cccgagtgga attggatcgg cttcctgagg gcctgcatct
      601 tttcgtagat ggggaagagc atctcgtcgg tgggcgacca gtggcgcggg atcatcttga
      661 tcccacgcag gcccttctcg acgatgccgt actcgatctc ctcgaccatg cccggtgtgc
      721 gcggctcggc ccagatcagg ccgtagatgc ggtcgggatc ggcggcctgg atctgggcga
      781 tgtggtcgat ggactcatga gcgtcgtcgc gacggtactg gccgtctacc ggaccgggat
      841 agcgggcgaa gaggttgatg cggtcgatcc tggcgtcgtc catccagcgc aggacctggt
      901 cggcggtctc gttgcctcgg gcgtggcagt ggcagtccca aatcctcatc tacccatatc
      961 ctccaaatcg cacgacggct tcatggaacg cgatgacgct ggcccaaggg acctcgtcgg
     1021 tgagggtgtg acacggggcg gcgatgtagc cgccgccggg ggccatgatg gccagggcat
     1081 cccggacgcc ggccatcacc tcgtcgggct cggtccgggc gaggatgctc tgggccccta
     1141 ccccacccca gaagctgatg tcgcgtccga actcgcgctt gagttgagcg atgtccatgg
     1201 cctcggcctg gaccgcttcg agcacgtcga tgcccatctc gatcagatcg gggatcagcg
     1261 gcgtgacgtg cccgcagcag tggaaggcga tctgggccga cgggccgcgc cagcggatat
     1321 gctcgacgag ctcctggtgt cgtggacggt accactggcg gtagtgctcg gggctcatga
     1381 cgagtgagcg ctggcttccg aggtcatcgc ccatccacac gatgtcgacg ccagcctctg
     1441 cgtaggcgcc gtagatggag gctttccatt cggtgatccg gtcgagcgtt cgcgcaaggc
     1501 cggcggggtc ctcgaaaggc gccacgaggg cgccctcgtc accgagccag gctttggcct
     1561 gctcgaacac gccgcacgcg taggccccgc agacggccag cccgcgaccg tgaagtcggg
     1621 cggtggcgac ggctgttgcg gcgatgtcag cgggcgttgg ggtgggcgtc cactggtctt
     1681 ccgggacgtg ggcgggcctc tcggggagtg ccaggctcac ataccggaca tcatggccga
     1741 agtggtcggc gaagtcgccc tcgcccacgt gtgcacggaa ctgcaggagt agctcgtggg
     1801 aagggcgcag gttgtagggc acgcgatcgg gttcgcggcg ggcgatggtc gtgagaacac
     1861 gctcgcggga agtcatggag tgaggtccta tcgtcggcta ccgcacccgg tgtcacggcg
     1921 ggtggggagt tcggcttcgg agccggtgtg tcctctgggc ggggatttgg cgtccgggac
     1981 aagtatggcc gatgggtgcc gtcaggactg cgcggggttg gttgcgagac ccgtaggggt
     2041 tgtggagggt tacaggagat ggcagcagta tggctgagaa ccgtcgtgtt gtcgtcgcag
     2101 ggcgtgtggt aggccttcgg gcgggagtgg gaggattcga cgcgtgaggg gagccttgtg
     2161 gggcgcggtg gcgtttattg ccgtctcgag cgcggtcgac gcacgggcgc cggccgtggc
     2221 gcctttcgaa cggccggatg aggtcgtgcc cgcgacgcgc attgatgagc tcgtcggtgc
     2281 ctcgctgcag gttgcgggca tcgagtcggc ctacctctgc tccgacgagg tgttcgtgcg
     2341 ccgggcacac ctggatatca tcggcacgct gccggagccc ctggttgtgc gcgccttcct
     2401 gcaagaccgg cggccggaga agcgcgcgag actgatcgac tcgctgctgg agcgcccgga
     2461 gttcgccgac tactgggcga tgaagtggtg tgatgtgctc cgggtcaagg cggagtttcc
     2521 catcaacctg tggcccaacg cggttcagtg ttaccagcgc tgggtccgcg aggccatggc
     2581 caccaacaag ccctacgacc tgttcgcgcg cgagctgctg acctcgagtg gcagcgcttt
     2641 ccgggtaccg ccggcgaact tctaccgtgc agtgcagggc cggaagcccg aggacatcgc
     2701 cggagccgtg gcgctgacgt tcatggggac gcggcttgcg agttggccgc agaccgagcg
     2761 ggagaacctg gcgcgcttct tctttcgggt ggcgtacaag aagaccgacg aatggaaaga
     2821 ggagatcgtg tgcctcgacc cgacgccgct ggggccgttc gacgccactc tcccggatgg
     2881 cacagcggtg catattgcgg ccgaccaaga cccgcgacag gtcttcgccg actggctgct
     2941 ggcggatggc aatccatggt tcgcgcgagc tatcgtcaac cgcgtgtggg cctggttgct
     3001 cggccgtggg attgtcgagg agccggatga catccggcct gacaacccgc catcgaaccc
     3061 cgagctgctg gcctaccttg a
//
LOCUS       DAOSPL010000435         3079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31764_length_3079_cov_3.350860, whole genome shotgun sequence.
ACCESSION   DAOSPL010000435 DAOSPL010000000
VERSION     DAOSPL010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31764_length_3079_cov_3.350860"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..523
                     /gene="rnpB"
                     /locus_tag="PLQ54_08940"
     ncRNA           140..523
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLQ54_08940"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            692..1915
                     /locus_tag="PLQ54_08945"
     CDS             692..1915
                     /locus_tag="PLQ54_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS93425.1"
                     /translation="MGISIGMVGLGSFGTAFADMFMSHPMVDRIALCDREPDRMAPYL
                     HKESWRAKLKPTDLYPSLDEILRTDLDALVIITQPWLHAEQCMKALEAGKHVYSAVPI
                     VSLPDGQEILDWCDRLVETCRRTGLHYMLGETTYYRPTAQFCRRMAAQGAFGDFVYAE
                     GEYFHDVDCPGCDLREVQRSRTSSTSGKEWLEEEKRYERRGVEGGPMHYPTHSTSGPV
                     SVMRAHAVKVTCYGYRNRNGDGYFGNGQISNETALFEMSNGAAVRICEFRECAGELGM
                     DGETFRIMGTRATFRENQWICNHRDTPPADMSKFERVTLTDEEMFEPLPPEVAAAFKD
                     ALGIQGEGDFIPSGHGGSHPYLVHEFVDAVAHDRHPTTNIWEAARYMVMGVMAHQSAL
                     KDGERLDVPDWGDAP"
     gene            complement(1937..>3079)
                     /locus_tag="PLQ54_08950"
     CDS             complement(1937..>3079)
                     /locus_tag="PLQ54_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOS93426.1"
                     /translation="IGFRDGITGLVPADWEVADRVRAAGKPTILAINKMESPKRDEYE
                     FAAFGIDPAIPVSAKARMNIPYLLDTIVAKLPSEDEGEPGAEIAYDFAATIVGRPNVG
                     KSSLLNRLLGQERSIVSEIPGTTRDAIDSLLEWDGHRILLTDTAGMRRKAKVKEDVEY
                     YSVVRALRSIDRSDLVLLMIDATEGITEQDQRIAGYAHEKGRLVILLVNKWDLLTDPV
                     ELDLPYKLDDPLTPTQVAKLERRLQGDWKHSVRDKLVFIDYSPVLFISALKGRGIDAI
                     LPQMVESIGQYRTRVPTPKLNRLMVDAAAAHPAPMRRGRRLKVYYATQAETSPPTIIL
                     FVNDPELMHFSYERYLRNRIREVFGLTGTPIRLMVRQRRRVERDGA"
BASE COUNT          530 a          972 c         1054 g          523 t
ORIGIN      
        1 cgaggggatg gttctgatgc aacaggggct caccgtccga ctggaagggg ccctcacctc
       61 ggagctgctc gtgatcgagg gtgtgtagcg tggcgctgcc cttccccctc gccggcccgt
      121 agggtacact cgcctatgga gagtgggccg tgcggccgcg ggccatgccg ttcgcggcag
      181 gctcgaggaa agtccgaact ccagagggcg ggacgcctgc taacggcagg gaggggcgac
      241 cctacggaaa gtgccacaga aacataccgc cgggccggct cgccgggccg gtaagggtga
      301 aatggtgagg taagagctca ccggcgcgat ggagacatcg cggccgggca aaccccgtcc
      361 ggagcaagac cgtgcagggg agccaggccc tggggtctgg cagcggtggc ccgccgctcc
      421 ccgggtaggt cgctagaggt gtctggcaac agccacccaa gatagatggc cgcagccggg
      481 cttcggcccg gtacagaatt cggcttacag gcccactctc ccaagccgcc cctgcctctc
      541 acctcgatga ccgtgccgtg ggcggccctt gtcaccgccg tcggcggtcc gcgacaaacg
      601 ctcccggcca cgcgcccgtt ggaggcgccg aggccccacg gggcgaagcg ccgctggcca
      661 ctgccccatc tcatctggag gtttcgccat catgggaatc agcatcggca tggtcggtct
      721 tggatccttc ggcacagcct tcgcggatat gttcatgagc cacccgatgg tcgaccgcat
      781 cgcgctctgc gaccgggagc ccgatcgcat ggctccgtac ctgcacaagg agtcatggcg
      841 tgcgaagctt aagcccaccg acctataccc ctcgctcgac gagatcctca ggaccgacct
      901 cgatgccctg gtcatcatca cgcagccctg gctccatgcc gagcagtgca tgaaggccct
      961 cgaggccggg aagcacgtct atagcgccgt gcccatcgtg tcgcttcccg acggccagga
     1021 gatcctcgac tggtgcgacc ggctggtgga gacgtgtcgg cgcacagggc tgcactacat
     1081 gctcggcgag acgacctact accggcccac ggcgcagttc tgccggagga tggccgcgca
     1141 gggcgcgttt ggagacttcg tctacgcgga gggcgagtac ttccacgatg tggactgccc
     1201 cggatgtgac ctgcgcgagg tgcagcgatc gcgaacctca agtacctccg gcaaagagtg
     1261 gctcgaggag gagaagcgct acgagcggcg aggcgtcgag ggcggcccga tgcactatcc
     1321 gactcactcg acatcggggc ccgtatcggt gatgcgcgcg cacgccgtga aggtcacgtg
     1381 ctacggttac cgcaaccgga acggcgacgg ctacttcggt aatggacaga ttagcaacga
     1441 gaccgcgctg ttcgagatga gcaatggtgc cgcggtgcgg atctgcgagt tccgcgagtg
     1501 tgcgggtgag ctgggcatgg atggcgagac tttccggatc atgggcaccc gggcgacctt
     1561 ccgcgagaac cagtggatct gcaaccaccg cgacacgcca cctgccgaca tgtcaaagtt
     1621 cgagcgcgtg acactcaccg atgaggagat gttcgagccg ctgccgccgg aggtcgcagc
     1681 agccttcaaa gatgccttgg gcatccaggg cgagggcgac ttcatcccat cgggccacgg
     1741 cgggtcgcat ccgtacttgg tgcacgagtt cgtcgatgcc gttgcgcacg accggcaccc
     1801 gacgaccaac atctgggagg ctgcgcggta catggtgatg ggcgtcatgg cccaccagtc
     1861 ggcgctcaag gatggcgaac ggctggatgt gccggactgg ggcgatgcgc cgtagggagc
     1921 cgggcgcggc cggcgctcac gccccgtctc gttccacccg ccgcctctgc cggaccataa
     1981 gccggatcgg tgtgccggtc aggccgaaga cctcacggat gcggttgcgc aggtaacgtt
     2041 cataggagaa gtgcatcagc tcggggtcgt tgacgaacag gataatggtc gggggcgagg
     2101 tctccgcctg agtggcgtag tagactttga gccgacggcc gcgtcgcatg ggggccggat
     2161 gggcggccgc tgcatcgacc atgaggcggt tgagcttcgg cgtcgggact cgagtgcggt
     2221 actggccgat ggactccacc atctgcggga gtatcgcgtc gatgccgcgc cccttcaggg
     2281 ccgagatgaa gaggacgggg ctatagtcga tgaacacgag cttgtcgcgc accgagtgct
     2341 tccagtcgcc ctgcaggcgc cgctcgagct tcgcgacctg ggtcggcgtt agcggatcgt
     2401 ccagcttgta ggggaggtcc agctccaccg gatcggtcag cagatcccac ttgttgacga
     2461 gcaggatcac caggcgaccc ttctcgtggg cgtagcccgc gatccgctgg tcctgctcgg
     2521 tgatcccctc ggtggcgtca atcatgagga gcaccaggtc ggatcggtcg atcgacctga
     2581 gcgcccggac cacgctgtag tactcgacgt cttccttgac cttcgccttc cgccgcatgc
     2641 ctgccgtgtc cgtgagcagg attcggtggc cgtcccactc gagcaggctg tcgatggcat
     2701 cccgcgtggt gcccgggatc tcgctgacga tggagcgctc ctgccccagg agccggttca
     2761 gaagcgatga cttgcccacg ttcggtcggc cgacgatggt ggcggcgaag tcataggcga
     2821 tctcggcgcc gggttcgccc tcgtcctcag acggcagctt ggcaacgatt gtgtcgagta
     2881 ggtagggaat gttcattctg gccttggccg acaccgggat ggccggatcg atgccaaacg
     2941 cggcaaactc atactcgtcg cgcttgggag actccatctt gttgatggcg agaatggtgg
     3001 gtttgcccgc agcccgtacg cggtccgcga cctcccagtc ggcaggaacg aggcccgtga
     3061 tgccgtcgcg gaagccgat
//
LOCUS       DAOSPL010000436         3071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31877_length_3071_cov_2.642573, whole genome shotgun sequence.
ACCESSION   DAOSPL010000436 DAOSPL010000000
VERSION     DAOSPL010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31877_length_3071_cov_2.642573"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLQ54_08955"
     CDS             <1..185
                     /locus_tag="PLQ54_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93427.1"
                     /translation="AWLGVFAHNQTVHLVAGEYEILPENALKGTPARPVEVKPLQNTT
                     IALEAPTRPVAPAAEH"
     gene            542..2140
                     /locus_tag="PLQ54_08960"
     CDS             542..2140
                     /locus_tag="PLQ54_08960"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HOS93428.1"
                     /translation="MNLQRPNASDVTGTSNRSRDVAPSSGLCARCLDSCKGNCEVFKA
                     SMRGRELIYPAPFGELTAGGDKEYPVDYSHLNILGYALGAEAIDEANPDKAIFPEVDT
                     TAVYGHMNPVKMRVPIFTGALGSTDIAMKHWESFAVGAAISGVSLVCGENVCGIDPGL
                     ERDANGRVRHSPEMARRVEVYRRWQEGFGDIIVQLNVEDTRLGVAEYVVEKLGVETIE
                     LKWGQGAKCIGGEIKIRELERAQELQRRGYIVTPNPSDPAVQSAFRQGAIKELERHSR
                     LGFIDQEGFCRAVEYLRRNVGVKRVSLKTGAYGMRELAMAIKWSSQARVDLVTIDGAG
                     GGTGMSPWRMMQEWGIPTFYLQAMAYECAKRLASRGEWVPDLAMAGGFSTEDHIFKVL
                     AMGAPYFKAVCMGRALMIPGFVGDNIAAVLNSGQTEWDGLPQSVSRFGETPEQIYVTY
                     ATLKEKYGDRIKDIPLGAIGLYTFIDKLRTGLTQLMAGARSFRLDTLGRDDLVALTED
                     AAKVSGIQYIMEAQREEALAIMDS"
     gene            2498..>3071
                     /locus_tag="PLQ54_08965"
     CDS             2498..>3071
                     /locus_tag="PLQ54_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93429.1"
                     /translation="MANALAFALFRALSLPATDLASFDGQLPAGWLIPEPTSWVVSGG
                     VLRCTDDSGERLALAPSDPLAEAMVQVDFRFIGGPRRSAGLVFGYTDPGEFSLLRRYD
                     SKAWLERLQIRGGEVTRVGGNHWLAQSGPGSAPVADGQWYTLKLCLVARTEDSHTWER
                     PENLGVFESMMGCAVSAGGKSSMSTDEHDVP"
BASE COUNT          529 a         1024 c         1000 g          518 t
ORIGIN      
        1 gtgcctggct cggcgtgttc gcccacaatc agacggttca tctcgttgcc ggcgagtacg
       61 agatccttcc cgagaacgct ctcaagggca cacctgcccg gcctgtcgaa gtcaaaccgc
      121 tccagaacac caccatcgcg ctcgaggcac cgacgcggcc cgtggcgcca gcagccgagc
      181 attagccggc accggttggc ggtcccacga ccctgtgcca cccctgaggc ccacccctcc
      241 gtcgctcggg gcacggtagc ttccggcgaa tgaccggcga attgcggcac gcaagcacat
      301 gaaggccagt ggatatccgg aaggccttcc ggtccaacgg ccatggaaga cggcgatcac
      361 gctctggtac gcctctaata cgacggaaat ataccgcttt ggccgtcttg accaggcgag
      421 gccgtgtcca tagactcggc cactaattgc cggaaagcaa tgtgcggctg ggaaccagcc
      481 accatggcgc cgggacacat ccaagagtgc cctacggctg ctcgaaccgg aggccaaacc
      541 catgaacttg cagagaccga acgccagcga tgtcaccgga acgtcgaacc gctctcggga
      601 tgtcgctccg agctcagggc tgtgtgctcg ctgcctggac agctgcaagg gcaactgcga
      661 ggtcttcaaa gcatcgatgc gtgggcgcga gctcatctac cccgctccct tcggtgagct
      721 caccgccggg ggcgacaagg agtacccggt cgactactcc catctcaaca tcctgggcta
      781 tgccctgggc gccgaagcca tcgacgaagc caaccccgac aaggcgatct tccccgaggt
      841 cgacaccacc gccgtctacg gccacatgaa cccggttaag atgcgggtcc ccatcttcac
      901 cggtgccctc ggctcgacgg acatcgctat gaagcactgg gagagcttcg ccgtgggcgc
      961 tgccatctcg ggtgtgtcac tggtgtgtgg cgagaacgtc tgtggcatcg atcccgggct
     1021 cgagcgtgac gccaatggcc gagtccgcca ctcacccgag atggcccgac gtgtcgaggt
     1081 ctatcgccgg tggcaggagg gcttcggcga catcatcgtc cagctcaatg tcgaggacac
     1141 gcggctgggt gtggccgagt atgtcgtcga gaagctcgga gtggagacca tcgagctcaa
     1201 gtggggccag ggcgccaagt gcattggtgg cgagatcaag atccgcgagc tcgaacgtgc
     1261 ccaggagctc cagcggcgcg gctacatcgt gactccgaac ccgtccgacc ccgcggtcca
     1321 atctgccttc cggcagggcg ccatcaagga gctcgagcgg cattcacgtc tggggttcat
     1381 cgaccaggag gggttctgcc gtgccgtcga gtacctccgc cggaacgtcg gcgtgaagcg
     1441 cgtgtcgctc aagaccggcg cctatggcat gcgcgagctc gccatggcca tcaagtggtc
     1501 ctcacaggcc cgggtcgatc tggtgaccat cgatggtgcc ggcggcggca cgggcatgag
     1561 cccgtggcgc atgatgcagg aatgggggat cccgaccttc tacctccagg ccatggccta
     1621 tgaatgtgcc aagcgtctgg cttcgcgcgg cgagtgggtt cccgacctcg ccatggctgg
     1681 cggcttctcc accgaggatc acatcttcaa agtcctcgcc atgggtgcgc catacttcaa
     1741 ggccgtctgc atgggccggg ctctcatgat cccgggcttc gtcggcgaca acatcgccgc
     1801 ggtcctcaac tcaggccaga ccgagtggga cggcctgccg cagagcgtgt ctcgcttcgg
     1861 agagacaccc gagcagatct atgtcaccta cgcgaccctc aaggagaagt acggcgaccg
     1921 catcaaggat atcccgctgg gggccatcgg actgtacacg tttatcgata agctccgcac
     1981 gggattgacc cagctcatgg ccggcgcccg aagcttccga ctcgataccc tgggccggga
     2041 cgacctcgtg gcccttacgg aggacgccgc caaggtctca ggcatccagt acatcatgga
     2101 ggcccagcgc gaggaggcct tggccatcat ggactcctag ctggaatgac tggccgtggt
     2161 cgtggcgcac gactcggtcg cgctgaatgt cctgactggt cctgcggtgg ccgcacaggg
     2221 gctgcccgca ggaggtgggg cctctgaccc tgagcctgtc gtaggacgcg gcgccgacgg
     2281 cccttgatgg gccggggagc gccatcgcgg agcactgacg gcccgggtcg gtcgccaggt
     2341 cgatcgatcc gggcccattg gcgcccacca tagggcttcg cgcgaccgtc ttccccatgg
     2401 ggcgcccccc ggtggggggc tcgaggtgga ccgcggagga gcgcgctcgc cggcgacgaa
     2461 gctcccgcgc agccccagtg gagaaggagt tgtcgccgtg gccaacgctc tggcgttcgc
     2521 gctgttccgc gctctcagcc tgcctgccac cgacctggcc tcgttcgatg gccaactacc
     2581 tgccgggtgg ctgatccccg agcccaccag ctgggtggtg tccggcggcg tcctccgctg
     2641 cactgacgac tcgggcgagc ggctggcgct tgctccctcg gatcctctgg cggaggccat
     2701 ggtgcaggtc gactttcgct ttatcggcgg tccgcggcgg agtgccgggc tcgtcttcgg
     2761 gtacacggac cccggcgagt tctccctgct ccggcgttac gacagcaagg cctggctcga
     2821 gcggttgcag atccgcgggg gcgaggtcac tcgcgtcggc ggcaatcact ggctcgccca
     2881 gtcgggacct ggctcggcgc ccgtcgccga cggacagtgg tacacgctca agctctgtct
     2941 cgtcgcaagg actgaagatt cgcacacctg ggaacgaccc gaaaacctcg gcgtcttcga
     3001 gagcatgatg ggttgcgccg ttagcgccgg cggaaagtcc agcatgtcca ccgatgagca
     3061 cgacgtgccg g
//
LOCUS       DAOSPL010000437         3067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31906_length_3067_cov_6.361886, whole genome shotgun sequence.
ACCESSION   DAOSPL010000437 DAOSPL010000000
VERSION     DAOSPL010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31906_length_3067_cov_6.361886"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PLQ54_08970"
     CDS             complement(<1..284)
                     /locus_tag="PLQ54_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93430.1"
                     /translation="MSAARRVVGCVATVVLVLMPGLLGGCHRQTGPNVSRSVAVPTAQ
                     SVPTRMAPPGDVVAVGAALAVAANGPHIVMPTASGSNITPSTAYPHAFVRA"
     gene            complement(829..2859)
                     /locus_tag="PLQ54_08975"
     CDS             complement(829..2859)
                     /locus_tag="PLQ54_08975"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOS93431.1"
                     /translation="MTECRWGRGARALAPALLVVLGILAPGGLASAQGRVTVDDLLRE
                     MVDMERLATAPEPGVECDQQSSYDRASTKPHAPDWFANGDAGQFLREEERNGAKEWVM
                     SEMTGPGAILRLWSANPDGGGTVRVYIDDMTTPALEEDFLALTSAQLAEFPSPFSGRR
                     SLGANLYFPMPYQTKCKVTVSKPGLYHHVGYRTFPAGTQVEPFSMAVLPRVKATMDEV
                     GALLSDPLTRLAATKGCRREGTTIVLGPRQSSRWVLKGPAAVTRFEVRPLTSKDVLTD
                     PLKKSILMVRWDDEDNPSIWAPLGDFFGSTPGINPYSSLPVGMTEDGLMYSNWFMPFE
                     KRAEVVIRNDSKTPLTLQVAAWLKPIDWDAKPWIHFHAKWWNQWLPAEPVFVDWPLLE
                     CDGGPGRFVGTMLGVMNTHQGWWGEGDEKFWVDNDTFPSYFGTGSEDYFGYAWCNPEL
                     FTHAYHNQSICTGPANFGYTAVARYHILDDIPFRHRLRMYIEKWDAAPREYCSTSYWY
                     APLGGTDMYRRPPEIVRGVLPLPEPFHVEGAIEGEKLEILEVTGGTTEVQGLSGDFSH
                     AEHLWWRNPKEGDTLELKLPVDKAGRYQLILGLVKSWDYGIHQFLLDGKPMGDPQDLI
                     ADQVTPFRLDLGSVDLAKGDHTLTVKCMGTNPNARPHNFMCGLDYVLLKPAG"
BASE COUNT          495 a         1046 c         1015 g          511 t
ORIGIN      
        1 gcccggacga aggcatgggg gtacgccgtg ctcggggtga tgttggagcc gcttgccgtc
       61 ggcatgacga tgtgcggccc gttcgccgcc acggcgagcg cagcaccgac ggctaccaca
      121 tccccgggtg gggccatgcg cgtaggcacg gactgggccg tgggtaccgc gacgctgcgg
      181 ctcacgttcg gccccgtctg ccgatggcag ccccccagga ggcccggcat gaggaccaga
      241 accacagtgg cgacacagcc gacgacacgt ctggctgcgg acaaggctcg cactcccctg
      301 cacggcggtt ctcgccgacc acctccggcc gcgctcaggc ttcgactgtc actcggggct
      361 gcgggttcca tacgccttgc cggctttggt ggcccacggc cgagggtagg tatggcttgc
      421 gacatggatc ggcaaacgac gcgatcctga acggcgttcc tccgctcgcc aaggtccctc
      481 gctccgcttc cgagcagacg gtctctggct aacgaggagc gtcgggcgga aaccgatgac
      541 agtcacgaat ggcacgcact tggggtcggc acccacgcac acccaaccgt aggcgaggct
      601 tcagcctcgc gcgaagtggc tctacaggcc attgtgagcc gaggctaaag ccccggctac
      661 agaggtgatg cgacatacgc ctctggcatt gggcttaagt gcggaccatt catgacagtc
      721 acccatctcc ggaattcaag agatgggtga ctgtcaccgg tttgcgctct gtgccgcctg
      781 ctgatccatg cctgcgggcc gtctggccac acctgcactt gcgccgcgct acccggccgg
      841 cttgagcagc acgtagtcca gcccgcacat gaagttgtgc gggcgtgcgt tcgggttcgt
      901 gcccatgcac ttgacggtca gcgtgtggtc ccccttggcc aagtccacgc tgccgaggtc
      961 cagcctgaac ggcgtcacct ggtcggcgat cagatcctga ggatcgccca tgggcttccc
     1021 gtcgagcagg aactggtgga tgccgtagtc ccatgacttc accaacccca ggatgagctg
     1081 ataccggccc gccttgtcga cggggagctt gagctcgagc gtgtcgccct ccttcgggtt
     1141 cctccaccac aggtgctcgg catggctgaa gtcccccgac agcccctgga cctcggttgt
     1201 gccgcccgtg acctctagga tctccagctt ctcgccctcg atggcgccct caacgtggaa
     1261 cggctcgggc aggggcagca cgccgcgcac gatctccggc gggcggcggt acatgtccgt
     1321 cccgcccaac ggcgcatacc agtaagacgt cgagcagtac tcccgcggtg ccgcgtccca
     1381 tttctcgatg tacatccgca gccggtggcg gaagggaatg tcgtcgagga tgtgatagcg
     1441 cgcgacagcc gtgtacccga agttcgccgg ccccgtgcag atgctctgat tgtggtaggc
     1501 gtgcgtgaac agctcggggt tgcaccaggc gtagccgaag tagtcttccg atccggtgcc
     1561 gaagtagctc gggaaggtgt cgttgtcgac ccagaacttc tcgtcgccct cgccccacca
     1621 gccctgatgg gtgttcatga ccccgagcat ggtaccgacg aagcgccccg ggccgccgtc
     1681 gcactcgagc aggggccagt ccacgaaaac cggctcggcc ggaagccact gattccacca
     1741 cttggcatgg aaatggatcc atggcttggc gtcccagtcg atgggcttca gccacgcggc
     1801 cacctgcagc gtcagcgggg tcttggagtc gttccggatc acgacttcgg cacgcttctc
     1861 aaacggcata aaccagttcg agtacatcag gccgtcctcg gtcatcccca caggcagcga
     1921 cgagtagggg ttaatgcccg gcgtcgagcc gaagaagtcg ccgagcggcg cccagatcga
     1981 gggattgtcc tcgtcatccc accggaccat caggatggac ttcttgagcg ggtcggtcag
     2041 cacgtccttg ctggtcagcg gccggacctc gaaccgcgtc acggccgccg ggcctttgag
     2101 cacccagcgt gaggactgcc tcggaccgag aacgatggtg gtcccctcgc gccggcagcc
     2161 cttggtcgcg gcgaggcggg tcagcggatc cgacagcagc gcgccgacct catccatggt
     2221 cgccttcacc cgcggcagca cggccatcga gaacggctcg acctgggtgc cggccgggaa
     2281 agtgcggtag cccacatggt ggtacagccc cggcttcgac accgtgacct tgcacttggt
     2341 ctggtagggc atggggaagt agaggttcgc gccgagcgac cggcgcccgc tgaaggggga
     2401 ggggaactcg gccaactggg cgctggtcag cgccaggaag tcctcctcga gcgcgggggt
     2461 ggtcatgtca tcgatgtaca cccgcaccgt tccgccaccg tcggggttgg ccgaccacag
     2521 acggaggatt gcgccggggc ccgtcatctc cgacatgacc cattccttgg cgccgttgcg
     2581 ctcctcctcg cgcaggaact gcccggcatc gccgttggcg aaccagtccg gcgcatgggg
     2641 cttggtgctc gcgcggtcgt agctcgactg ctgatcgcac tcgacgccgg gctccggcgc
     2701 cgtggccagg cgctccatgt cgaccatctc gcgcagcagg tcatccaccg tgacgcgccc
     2761 ctgggccgag gcgagcccgc ctggcgcgag gatcccgagt accacaagca acgcgggggc
     2821 cagcgcacgc gcgccgcgcc cccatctgca ttccgtcatc gtgagttccc ctccatcggt
     2881 gatgcacaaa tcgccagctg cagggttcgc ggcaggctac ggggagccct ccgtcgccgg
     2941 gcgacggcgt gagttggagg gcggccccga ggcgcccggg cggctcgggg ccagggttac
     3001 cgcgctcctc cacccggccg tgagccccct cgggcacagg tagtccgggc tggacagggc
     3061 actggcg
//
LOCUS       DAOSPL010000438         3066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31932_length_3066_cov_3.759548, whole genome shotgun sequence.
ACCESSION   DAOSPL010000438 DAOSPL010000000
VERSION     DAOSPL010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31932_length_3066_cov_3.759548"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLQ54_08980"
     CDS             <1..256
                     /locus_tag="PLQ54_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93432.1"
                     /translation="AMGGRPQCLCAHLKRIGDDRSTTRPEDVSSRDSGPEQANWVCTH
                     GIYDRAWQAGSRPRPSVCFGPRPGNCPEFEPGPPSEEAGE"
     gene            253..2337
                     /locus_tag="PLQ54_08985"
     CDS             253..2337
                     /locus_tag="PLQ54_08985"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOS93433.1"
                     /translation="MNRSGRPEAELQTSLPKFLAGLSQTCREENRSARESIAAPEDWR
                     KARGNLEGALLDCLGGLPELREPLNSRLCGEVQGPGYRMAQVAYESLPGLHVSASLYL
                     PETEGQHPGVTIQCGLTSAGKAEAAIQRLAIELATNGMAALVADPVGQGERASYRTTD
                     GMVLPGGGACEQAYLDGPCRLAGASLVSYMVYECSRALDLLAALPAVDADHLGVVGHE
                     QGCLVALLAAALDERVGSTCLVAAPAWHEQVEAGRVLAPGGLLRSGLDLAALCALVAP
                     RPLRIIRGGPESDDGDDLSFARRVYALEGQPGNLDCVWCDGARDLSSSARARVREWLA
                     RSSGVGVVEEPLADPPWLPVEDTWVTPEGRIEELGGHSFFHIHRALARAQITARPSQD
                     PEQVAAAVSSIVGRGPAVASEPLTRPRSQEDGLQVEDVWLEVDEGLRCAGVVVGPEDR
                     RGGPGVVWLHGRGDAGLAAHWDRVRDLAGSGVRVLAFWPRGTGPEAPREIAGQSRLAA
                     SLLGVESQWARQALMCGQSLAAMRARDITSAVAYLISRPEVDSASVYVCAEGRPSLWA
                     LLAGVVESGIAGLVLKGLRSSFDGIATHSRHRFVLADVLPGVLSLLDVPDLVRSIAPL
                     PVALVSPLDHMGEPVSDHRAHVAFGHARRAYRALGAEDWFEIRTRTTPGSPDLDATAS
                     LRDLLRRAEGHP"
     gene            2334..>3066
                     /locus_tag="PLQ54_08990"
     CDS             2334..>3066
                     /locus_tag="PLQ54_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOS93434.1"
                     /translation="MRAPTVGDIADFMDRWAPPHLAEDWDRVGLQLGSRDQPVGRVLL
                     AVDLSSAVVEEALARAADLIVCHHPAIFRPVASLTDSSAQGRKLLSLARAGVSVFVAH
                     TNLDAAHAHSTSDALARAIGLFADVNPLVPPAQPPTLKLVTYVPPTSADTVFAALAEA
                     GAGCIGQYSHCSYRVTGHGTFLPGPGTHPAVGEVGRLETTDETRLETIVPPGALERVL
                     SALHSAHPYEEPAYDVFPLHTSASPA"
BASE COUNT          421 a         1060 c         1092 g          493 t
ORIGIN      
        1 tgccatgggc ggaagacctc agtgtctgtg cgctcacctg aaacgcatcg gggacgacag
       61 gagcacaacg cggcccgagg acgtctcgtc gcgtgactcg ggacccgagc aggcgaactg
      121 ggtatgcacg cacgggatct acgaccgcgc ctggcaggcg ggctctcggc ccaggccatc
      181 ggtctgcttc gggccccggc cgggcaactg cccggagttc gaaccggggc cgccgagtga
      241 agaggcaggg gaatgaaccg atccggcagg cccgaggccg agttgcagac gagcctccca
      301 aagttcctgg cgggcctgtc gcagacctgc cgagaggaga accgctcggc ccgcgagagc
      361 atcgcggccc ctgaggactg gcgcaaggct cgaggtaacc tcgagggcgc acttctcgac
      421 tgcctcgggg gcctccccga gctgcgggag cccctcaaca gccggctgtg tggcgaggtg
      481 caggggccag gctaccgcat ggcccaggtc gcgtacgaga gtctgccggg tctccacgtg
      541 tccgcgagtc tgtacctgcc cgagaccgag ggacagcatc ccggggtcac catccaatgc
      601 gggctcacca gcgccgggaa ggctgaggcg gcgatccaga ggctggcgat tgagttggcc
      661 accaacggca tggccgccct ggtggctgat ccggtcgggc aaggcgagag ggcatcctac
      721 cggaccaccg acgggatggt gcttccaggc ggaggagcgt gcgagcaggc ctatctcgat
      781 gggccttgcc ggctcgccgg cgccagcctg gtgtcgtaca tggtctacga gtgctcgcgg
      841 gcgctggacc tgctggcggc actgccggcc gtcgatgcgg accacctcgg tgtggtcggc
      901 cacgagcagg gctgcctcgt ggcactgctg gccgccgccc tggacgaacg cgttggatcc
      961 acgtgcctgg tggctgcacc cgcctggcac gagcaggttg aggcgggccg agtgctggcc
     1021 cccggtgggc tgctccgatc tggtctcgat ctggcggctc tgtgtgccct ggtggctccg
     1081 cgcccgctgc gcatcatccg tggaggcccg gaaagcgacg acggtgacga cctgagcttc
     1141 gctcgccgcg tatacgccct cgaaggccag cccggcaacc tggactgcgt gtggtgcgac
     1201 ggcgcccgcg acctctcgtc atcggcacgc gcccgcgttc gcgagtggtt ggcgcggtcg
     1261 tccggtgtcg gagtggtcga agagccactc gccgatccgc cctggcttcc ggtcgaggac
     1321 acctgggtta ccccggaggg ccgcatcgag gaactgggcg gtcattcgtt ctttcacatc
     1381 catcgggcgc tggcccgcgc gcagatcacg gcgcgaccga gccaggatcc cgagcaggtc
     1441 gctgcggcgg tctccagcat cgtgggtcgc ggcccggcgg tggcctcgga gcccctgacc
     1501 cgcccgcgga gccaggaaga cggactccag gtcgaggacg tgtggctgga ggtcgacgag
     1561 ggcctgcggt gcgccggggt ggtcgtcggg ccagaggacc ggcggggcgg cccgggcgtc
     1621 gtgtggctcc acggccgcgg cgatgcggga cttgccgcgc actgggatcg tgtgcgtgac
     1681 ctggccggta gcggcgtccg ggttctcgcg ttctggcccc ggggcaccgg ccccgaagcg
     1741 cctcgcgaaa tcgccgggca gtcgcgcctg gcagcgtcgc tcctcggggt cgagtcgcag
     1801 tgggcacgcc aggcgctcat gtgcgggcag tcattggccg ccatgcgcgc ccgggacatc
     1861 acgtcggcgg tcgcctacct gatctcgagg cccgaagtgg actccgcatc ggtctacgtg
     1921 tgcgctgaag ggcgaccatc gctgtgggca ctattggctg gggtggtcga atcgggcatc
     1981 gcggggctcg tgctcaaggg attgcggtcg tcgttcgacg gcatcgcaac ccactcacgc
     2041 caccggttcg ttctggcgga cgtcctgccc ggcgtcctgt cgcttctcga cgtgcccgac
     2101 ctggtgcggt ccatcgcccc cctgccggtg gcgctggtct ctccgcttga ccacatgggc
     2161 gagcccgtct ccgaccaccg tgcgcatgtc gcgttcggtc acgcgcgtcg agcctaccgg
     2221 gcgctgggtg ccgaggactg gttcgagatc cggacccgca ccacgccggg ctctccggat
     2281 ctggatgcca cggccagcct ccgcgacctg ctgcggcgag cggagggtca tccatgaggg
     2341 cccccaccgt gggtgacatc gcggacttca tggaccgctg ggcaccgcct cacctggccg
     2401 aggactggga ccgcgttggc ctgcagctgg gcagtcgcga tcagcccgtc ggccgggtac
     2461 tcctggccgt ggacctgagc agcgcggtgg tggaggaggc gctcgctcgc gctgcggacc
     2521 tcattgtttg tcatcacccg gcgatcttcc ggccggtcgc ctcgctgact gattcctcgg
     2581 cccagggccg gaagcttctc tcattggctc gggcgggcgt ctcggttttc gtcgcgcaca
     2641 ccaatctcga cgctgcccat gcccacagca cctccgacgc cctggcgcga gcgatcgggc
     2701 tgttcgctga cgtgaacccg ttagttccgc cagcccaacc accgacactg aaactggtca
     2761 cctatgtacc tcccacatcc gccgacaccg tgtttgccgc gctggccgag gcgggggcgg
     2821 gatgtatcgg ccagtacagc cactgctcct accgggtcac cgggcacggg acgttcctgc
     2881 ccggaccggg gacccacccg gccgtgggcg aggtggggcg cctcgagacg accgacgaaa
     2941 cgcggctcga gacgatcgtt ccgcccggcg cgctcgagcg cgttctgtcg gcgctccatt
     3001 cggcacaccc ctacgaggaa cccgcctatg acgtgtttcc acttcacacg tcggcttccc
     3061 ccgcgc
//
LOCUS       DAOSPL010000439         3066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31934_length_3066_cov_3.594487, whole genome shotgun sequence.
ACCESSION   DAOSPL010000439 DAOSPL010000000
VERSION     DAOSPL010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31934_length_3066_cov_3.594487"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PLQ54_08995"
     CDS             <1..372
                     /locus_tag="PLQ54_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93435.1"
                     /translation="NRLYDFGRHHPCMVLMANGDLVMTYVVRLGYPRNADGLPQFGIE
                     AIVSRDNGASWDIAHRYVLAEWSGNRTGPNEWWASSQATSTVLLPDGSLLTAYGTGYR
                     SEPMENGLPGPRDVGLIHWRP"
     gene            491..1555
                     /locus_tag="PLQ54_09000"
     CDS             491..1555
                     /locus_tag="PLQ54_09000"
                     /inference="COORDINATES: protein motif:HMM:NF015368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93436.1"
                     /translation="MTRMPARIVLPRTPLLCVVSAALCLSAACMRQASGQETTSASAC
                     GQPGPHQVVTMTFDWADPARAGRRVPAKLYVPASTEAPCPVIIFSHGLGGTREGYEYL
                     GRHWASHGYVSVHIQHVGSDDSAWRGQRDTVAAMRRAAADPRNAIERPRDVRFAIDQL
                     ERLNTEEGELRGRLDLAHIGMAGHSFGAHTTLWVAGQPMLGPAGLQLPMGDPRVTAAI
                     AMSPAPPPRASDAAIRRAFGQIRLPCMHMTGTEDQSIITSTTPAQRRIPYDCCSGSDQ
                     YLCIYSGGDHMVYSGRGRLADRPRDPAFRQHTLELTTAFWDAYLRNDPAARTWLDGGA
                     AAASLGADGTLEQKHPAPQE"
     gene            complement(1963..>3066)
                     /locus_tag="PLQ54_09005"
     CDS             complement(1963..>3066)
                     /locus_tag="PLQ54_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93437.1"
                     /translation="RLGEFWDQGGLVICAGDIPANSTEDLRDARVGAASARMLGLAAR
                     EEAGEGGEIIANTNAAGGVAVYVPETLLPAVPRLLDQLLERDFSAGDASGALRYAHWS
                     IEGREAYYVINDSAAPVQAECTFSADGDAELWDPATGTISVGDASEASGGRAVSLSLE
                     PYSATFVVFPTAVERKRRELQGPLGPVASSTPLTDVVGAEVRFEAAGPEHVTRSTAPA
                     TAGMGGDRPGSVVIDATIQKGGIDSHCFASAKLPASTDLRGFEALRLRTFAPDDQAGC
                     GTSLLVLLHEKGGAVYLASAGRALSEPGWQESVIWLESFSLAGWSKDTNSRLDLDAIS
                     AISVGWGGYFGKDDERVRFVLAPVELIQFDWKA"
BASE COUNT          476 a         1107 c         1012 g          471 t
ORIGIN      
        1 aaccggctct acgacttcgg gcggcaccac ccctgcatgg tactgatggc caacggcgat
       61 ctggtgatga cctacgtcgt ccgcctgggc tacccgcgca acgccgacgg gctgcctcag
      121 tttgggatcg aggccatcgt gagccgcgac aacggcgcca gttgggacat cgcgcatcgg
      181 tacgtcctcg ccgagtggtc gggcaatcgc actgggccga acgagtggtg ggccagcagc
      241 caggctacgt cgaccgtact gctcccggat ggatctctgc tgactgccta cggaacgggc
      301 taccggagcg agccgatgga gaacggcctg cctggcccga gggacgtcgg cctcatccac
      361 tggcggccgt aggcatccgc gagcctccgc tggacagccg ccagggtcgg caacgggtag
      421 gtttccaggg ccgcccactt tccgagacct gcacggctct cgcgggttct atggaggaca
      481 ggaggtcgcc atgactcgca tgccagcccg gatagtcctg cctcgcacgc cgctcctatg
      541 tgtggtctcc gcggcgctct gcctcagcgc cgcgtgcatg cgccaggcat cgggccagga
      601 gaccacttcg gcgtcggcct gtggccagcc cggacctcac caggtcgtca cgatgacttt
      661 cgactgggcc gaccccgccc gcgcgggccg tcgtgtgccg gcgaagctct acgttcccgc
      721 gagcacggaa gctccgtgcc ccgtcatcat cttctcccac gggctgggtg gaacacggga
      781 gggctacgag taccttgggc gccattgggc gagtcatggc tatgtctccg ttcacatcca
      841 gcatgttggc agtgacgact cagcctggcg tggacagcgg gacactgtgg ccgcgatgcg
      901 ccgcgccgcg gctgacccgc gtaacgcgat cgagcgcccg agggatgtcc gcttcgccat
      961 cgaccagctc gagcggctga acacagagga aggcgaactc aggggccggc tcgacctggc
     1021 tcacatcggg atggccggcc acagcttcgg agcccacacg acgctctggg tggccggcca
     1081 acccatgctg ggcccggcgg gcctgcaact acctatgggt gacccgcgtg taacggcggc
     1141 catcgccatg agcccggcgc caccacctcg tgccagcgac gcggccatcc ggcgcgcgtt
     1201 cgggcagatc cgccttccgt gtatgcacat gaccggaacc gaggatcaga gcatcatcac
     1261 ctcgaccacg ccggcgcagc gacgcattcc gtatgactgc tgctcggggt cagatcagta
     1321 cctgtgcatc tactcggggg gcgaccacat ggtctattcg ggccgcggcc gcctggccga
     1381 tcggcccagg gaccccgcct tccgccagca cacgctcgag ctgaccacgg cattctggga
     1441 tgcctacttg cgcaacgacc ccgcggcccg aacgtggcta gacggcggcg cggccgcagc
     1501 ctcgctcggc gcggacggga ctcttgagca gaagcatcca gcgccccagg agtagccagc
     1561 gtgcggcgac cggctccgtt gccgatgtgg tgtcgttcct cggcacgtca ccgcagtcgt
     1621 gccgtccgat gctgccctgc cactgtatgg cggtgtagtc ctctccccca ccacgacaga
     1681 ggccgtcgtt tgggcctggc agacacacga gcggtgcacg gcggcgccgc cagcgtgtcc
     1741 aacgccaacg acatggtgca cctcattcac cgcccgccac ctcagtcgcc ctgcggacga
     1801 cctgagccgc ggaggcctcg ggctcctggg ggcgagtggt gcgggctcca tccgcaacgg
     1861 ccaacgcgcg cgagacgcga tccatcaggc aggcactggg ggggagacaa gccccctccc
     1921 ggcctctccc gcacacgagg gcggtgaaca gccagcgccg ctctatgcct tccagtcgaa
     1981 ctggatcagc tccacgggcg ccaggacgaa cctcactcgc tcgtcatcct tgccgaagta
     2041 cccgccccac cccacgctga tcgcgctgat cgcgtcgaga tcgagccgac tgttggtatc
     2101 cttcgaccat ccggccagcg agaagctctc gagccagatg accgactcct gccagcccgg
     2161 ttccgacagc gcgcggcccg ccgacgccag gtagaccgcg ccgcccttct cgtgtagcag
     2221 aaccaggagt gaggtgccgc atccagcctg gtcgtcgggc gcgaacgtac gcagcctgag
     2281 ggcttcgaag ccccgcaagt cagtgcttgc cggaagcttt gcgctggcga agcagtggct
     2341 gtcgatgccg cctttctgga tggtggcatc gatgacgacg gagcccggcc ggtcgccgcc
     2401 catgcctgcc gtcgcgggcg cggtcgagcg tgtgacatgc tcgggccccg cggcctcgaa
     2461 ccgcacctcg gcgcccacga cgtctgtcag tggcgtcgag ctggccacgg ggccgagcgg
     2521 cccttgcagc tcccgccgct tgcgttccac cgcggtcggg aacacgacga acgtggcgct
     2581 gtagggctcc agggacagcg acaccgcccg tccgccactg gcttcggagg cgtccccaac
     2641 cgagatcgtg cctgtggccg ggtcccacag ctccgcgtcg ccgtcggccg agaaggtgca
     2701 ctcagcctgg accggcgccg ccgagtcgtt gatgacgtag taggcttcgc gcccttcgat
     2761 gctccagtgg gcgtagcgga gcgcgccgga agcatcgccc gcggagaagt cgcgctcgag
     2821 caactggtcg agcagccgag gcacggccgg gaggagcgtc tcgggcacgt agaccgccac
     2881 gccgccggcg gcgttggtgt tggcgatgat ctctccgccc tcgcccgcct cctcgcgggc
     2941 cgcgagtccc agcatcctcg ccgacgcggc tcccacgcga gcgtcgcgca ggtcctccgt
     3001 gctgttcgcc gggatgtcac cggcacagat cacgagcccg ccctggtccc agaactcgcc
     3061 gagccg
//
LOCUS       DAOSPL010000440         3064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31969_length_3064_cov_3.090395, whole genome shotgun sequence.
ACCESSION   DAOSPL010000440 DAOSPL010000000
VERSION     DAOSPL010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31969_length_3064_cov_3.090395"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2159)
                     /locus_tag="PLQ54_09010"
     CDS             complement(<1..2159)
                     /locus_tag="PLQ54_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93438.1"
                     /translation="MRHWHALAALGVASLTGGSAAQESPSEFALEGVPYIGNDWQAHL
                     DGPQDLMFASAMASVTKFLGTAPPDAYRFFMGVCGVAQQQLWHPTEWRTAFDDVWRLS
                     DDPTESVRRTFEAAGYEYRLLGNQAVRDARGLPADYPEYVIPDVLKQQVCESLRSGVP
                     VIAFGLKSGAAVIAGYEENGDVLLGWAMLENEGASERGLENYFRLRQWTDETQGVALI
                     GQRVEPPSTDESCQRALLWALQNGRRARIGEYLSGLSALAAWADALVREPFPDPADLA
                     TLRRQHVSHFMTSLNVAEGRAFAYAMTDPVEQAHPEVAEDVGVTIQCHRLMHDLVWRL
                     WQTKEPGTEEEQLRRFADPAVRQELRRIVLLERDADAHAFAHIDHMLRTLGVDPKEIP
                     EPSADERDIVARADAREQASGGDRSALEHQEVNLWVGGVPRIAWHQGKDCTFIGALEA
                     AMAPTFRPCEYSDLMGYSGLAFRTRWFRNPEGVATPYGTLRWHPVSPHGEGPDEVAAI
                     SRATGWDLRRVDFAEDDHLARMKAITDFVLSINEALPVVIGYNTDLAVAYGYHIHSMS
                     LFLRDYQRPDADDIRVGSEDAKLGPVAVFLGGIGEAPTPADALLDGLAMAVSQGRQAR
                     GDGFVYGPDALRAWAGDLAGYDGYSPEEQSLLYQTNWWTLMHLADARRAAVAFLEANT
                     ELLPEAARSALAPALECYSQEAELLDGFVTANAQFLP"
     gene            complement(2186..>3064)
                     /locus_tag="PLQ54_09015"
     CDS             complement(2186..>3064)
                     /locus_tag="PLQ54_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93439.1"
                     /translation="SGACFRRVWNRDDGGNVDLMYFEPEPYERILNALGYEFRTVPRS
                     DKAAMVAAVKESIAAGRPVLATVLVGPPEVGVITGYDADGETLIGWSYFQDAALPGYC
                     EVPGWFEKLEGGPKVGLIVLGEKTGMVPTAEETLLSSLEWAVDLARTETRPGIPDHIL
                     GLAAYDGWADGLEVDADFTADDARVLETRRMVHGDQSVMLVERHSAAGFLRDMAAEVP
                     EVAEPLEEAARLYDEVGDLIGKVWPWADDKGPAVQQALVDSEQRRQVAEHIRAARGRE
                     AQAIGFLEQAIGMLKQ"
BASE COUNT          498 a         1114 c          969 g          483 t
ORIGIN      
        1 cagggaagga actgggcgtt cgcggtcacg aacccgtcca gcagctccgc ctcctgcgag
       61 tagcactcga gtgccggcgc cagagccgat cgggccgcct caggcagtag ttcggtattc
      121 gcctccagga acgccacagc cgccctacgc gcatctgcca ggtgcatcag cgtccaccag
      181 ttcgtctggt agagcaggct ctgctcctcc ggcgagtagc catcgtagcc agccaggtca
      241 cctgcccacg cccggagtgc gtccggcccg tagacgaatc cgtcgcccct cgcctgccgc
      301 ccttggctca cggccatggc gaggccatcg agcaatgcat cggccggagt cggcgcctcg
      361 ccaatgccac cgaggaacac ggccactggg ccgagcttcg catcctcgga acccacgcgg
      421 atgtcgtcag catccggccg ctggtagtcg cgcaggaaga ggctcatgct gtggatgtgg
      481 tacccatacg ccacggccag gtcggtgttg tagccgatca ccacgggcag ggcctcgttg
      541 atggacagca cgaagtccgt gatggccttc atccgggcca ggtgatcgtc ctcggcgaag
      601 tcgacccggc gcaggtccca ccccgtggcc cgactgatgg cagcgacctc gtcggggcct
      661 tcgccatggg ggctcaccgg gtgccaccgc agtgtgccgt aaggagtggc gacgccctcg
      721 gggttccgga accaccgcgt gcggaacgcc agccccgagt agcccatcag gtcgctgtac
      781 tcgcacgggc ggaaggtcgg tgccatggct gcctcgagtg cgccaatgaa cgtgcagtcc
      841 ttcccctggt gccacgcgat ccgcggaacg ccgcccaccc acagattgac ttcctgatgc
      901 tccagcgccg accggtcgcc gccgctcgct tgctccctcg cgtcggctcg ggccacaatg
      961 tcccgctcat cagccgaagg ctcggggatc tctttcggat cgactccgag cgtgcgcagc
     1021 atgtggtcaa tgtgcgcgaa ggcgtgcgcg tccgcatccc gctcgagcag cacgatgcgg
     1081 cgcagctcct gtcgcaccgc ggggtcagcg aaccgcctca gttgttcctc ctcggtgccg
     1141 ggctccttgg tctgccagag ccgccacacc aggtcgtgca tcagcctgtg acattggatg
     1201 gtcacgccca cgtcctccgc gacctccggg tgagcctgct ccaccggatc cgtcatggca
     1261 taggcgaagg cacgcccctc ggccacattc agtgaggtca tgaagtgtga aacgtgctgg
     1321 cggcggaggg tggccaggtc ggccgggtct ggaaagggct cccgcaccag cgcatcggcc
     1381 caggcagcca gggcggagag gccggagaga tactcgccaa tgcgcgcgcg ccgaccattc
     1441 tgtagggccc agagcagcgc ccgctggcat gactcatcgg tcgacggcgg ctcgacccgc
     1501 tgcccgatca gcgccacgcc ttgggtctca tcggtccact ggcgcaaccg gaagtagttc
     1561 tcaagcccgc gctcgctcgc gccctcgttc tcgagcatgg cccagccgag gagcacatcg
     1621 ccgttctcct cgtagcctgc gatcaccgcc gcgccgctct tcagcccgaa ggcgatgacc
     1681 ggcacaccag aacgcaggct ctcacacacc tgttgcttca gcacgtcggg tatcacgtac
     1741 tccgggtagt cggcagggag cccacgagca tcgcggaccg cctgattgcc caagaggcgg
     1801 tactcgtacc cggcggcctc gaacgtgcgg cgaaccgact cggtggggtc gtccgatagc
     1861 cgccacacgt cgtcgaacgc cgtgcgccat tccgtcgggt gccacagctg ctgctgcgcg
     1921 acgccgcaca cgcccatgaa gaaccggtac gcgtcgggcg gagcggttcc caggaacttc
     1981 gtcaccgacg ccatggccga agcgaacatg aggtcctgcg gcccgtcgag gtgcgcctgc
     2041 cagtcgttgc cgatgtaggg gacgccctcg agagcgaact cggacggcga ttcctgtgcg
     2101 gcggagccac ccgttagcga cgccacaccc agtgcggcca gcgcgtgcca gtggcgcatg
     2161 cgacgcctcc catcgctgtc acgcgctact gcttcagcat cccaatggcc tgttcgagga
     2221 acccgatggc ttgggcctcg cgcccccggg ccgcacgaat gtgctcggcg acctggcgcc
     2281 gctgttcgga gtcgactagc gcctgctgga cggcaggacc cttgtcatcc gcccacggcc
     2341 acaccttgcc gatgagatcg cccacctcgt cgtagagccg ggccgcctcc tcgagaggct
     2401 cagcgacctc aggcacctcg gcagccatgt cgcgcaggaa cccggcggcc gagtggcgct
     2461 cgaccagcat cacactctgg tcgccatgga ccatgcggcg cgtctccagc accctggcgt
     2521 cgtcagccgt gaaatcggca tccacctcga gcccgtccgc ccagccgtcg taggccgcca
     2581 ggccgagtat gtggtcgggg atgccgggac gcgtctcggt gcgggccagg tcgacggccc
     2641 actcgagcga tgagaggagc gtctcctcag ccgtcggcac catgcccgtc ttctccccga
     2701 gcacaatgag cccaaccttc ggcccgccct cgagcttctc gaaccagcca gggacctcgc
     2761 agtagccggg cagggccgca tcctggaagt aactccagcc gatgagcgtc tcaccatctg
     2821 cgtcgtaacc ggtgatgacg cccacctcgg gaggcccgac tagcacagtc gccaacaccg
     2881 gccgccccgc ggcgatcgac tccttgacgg ccgcaaccat cgccgccttg tcgctgcgcg
     2941 gaacggtgcg gaactcgtac cccagcgcat tgaggatgcg ctcgtagggc tcgggctcga
     3001 agtacatcag gtccacattg ccgccgtcgt ctcggttcca gacccgccgg aagcacgccc
     3061 cgct
//
LOCUS       DAOSPL010000441         3062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_31994_length_3062_cov_3.605920, whole genome shotgun sequence.
ACCESSION   DAOSPL010000441 DAOSPL010000000
VERSION     DAOSPL010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31994_length_3062_cov_3.605920"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="PLQ54_09020"
     CDS             <1..921
                     /locus_tag="PLQ54_09020"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS93440.1"
                     /translation="YDLERLGETFDVVICGGVLHHLSDPVRGLKCLRSVLKDDGVLSI
                     MVYGRYGRTGVYVLQDLIRMVSGGRSQSELAPWTRKLVGALPPAHAFHRRKFGDLDMA
                     GDSGLVDLLLHPQDRSYTVPEIYEWLEQASLELLQWCPRRQYEPALYVRDHDVRDSLS
                     ALSERDRAIAAELLSSDHAKHHFLACKPGFRVERPSIDDADWLSSRPVSNPHWALHQE
                     GEPGPDGSVTRGRLSAINLVAGVALRVDLAEEEARILRLCNGDHTAGEIADSRELRRR
                     FSRIGSEGRRQLLRRFLRRAIEHELVYLCP"
     gene            912..2228
                     /locus_tag="PLQ54_09025"
     CDS             912..2228
                     /locus_tag="PLQ54_09025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS93441.1"
                     /translation="MSVIAELAMDSPVAIGGSPPHWLPLAAGDRLGDLARHYDEYPHP
                     PYADHAPHRGVPPRDLLDLARYVLWPRRKVLADARVLDAGCGTGAQLAAMGMRHPDVE
                     LWGVDVSARSLDDAADRARRCNVGSVHLRQLPVERVGELGLEFDLVVLQGVLSASSDP
                     RATLSAAASVVRPEGGILVSVAAPYGRLGAEGFRELVDLARGGQPRAERLRFARKLRA
                     SREWHSRLLEAVLDSREEDDASLAELAVLDPPLRAYSVPELTGLLASAGLRIWRFHPR
                     TPYEPERYLPAPHHGCLRAVPEVLRPAVAELLHSAMHTHCFFAVREGFEPERVDLDGE
                     DWLDLQPIANGLWDFAASDGPVPEQAGRAGVATREFCGRCECIALTPWQRIALELCES
                     GTRVRDILRSRRLRQELAGLGRGDYCEAVRSFLRRAAHDELVYLVG"
     gene            2254..>3062
                     /locus_tag="PLQ54_09030"
     CDS             2254..>3062
                     /locus_tag="PLQ54_09030"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS93442.1"
                     /translation="MAVTPVVSAPGGLSDAVLAQYEAYPCPGHPPGTAEPEVPDWLGY
                     LRPLLWPRRQDLSGLKVLDAGCGTGAMTSGLARCHPEVNVLAIDISARSLEVAARRLG
                     GCQGGNVRLRQLPIERANELDERFDLIVVWDVLHCVIDAPRVLRTLGDLLTPDGALIA
                     SVRAPHGRAGAVALRSLMEALGLGLECPEAIPRARSIIDNLSATHLFQRSEWQQDLAA
                     GGDAAVVDLLLPARQRLYNALEVFELVAESGLEFLRWFCPNMYCPRRYLADA"
BASE COUNT          419 a         1023 c         1087 g          533 t
ORIGIN      
        1 tacgatctcg agcgtctcgg cgagacgttc gatgtggtga tctgcggcgg ggttctgcac
       61 catctctcgg accccgtgcg gggcctgaag tgcttgcgct cggtgctcaa agacgatggt
      121 gtcctctcga tcatggtgta cggccgctat ggccgcacgg gcgtgtacgt gctgcaggac
      181 ctgatccgga tggtctccgg cgggcgctcg cagtccgaac tggccccctg gacccggaaa
      241 ctcgttggcg ccctgccgcc ggcacacgcc ttccatcggc gcaagttcgg cgacctcgac
      301 atggcgggcg attccgggct ggtggacctg ttgctgcacc cccaggaccg cagctacacg
      361 gtgcccgaga tctacgagtg gctggagcag gcatcgctgg agctgctgca gtggtgtccg
      421 cgtcgccagt atgagccagc gttgtacgtg cgcgaccatg acgtgcgcga ctcgctgtcg
      481 gcgttgagcg aacgtgaccg ggccatcgcg gccgagctgc tgtcgtccga tcacgccaaa
      541 caccactttc tggcctgcaa gcccggtttc cgcgtggagc ggccctcgat cgacgacgcg
      601 gactggctct cgagccggcc ggtctcgaac ccacactggg ccctccacca ggaaggcgaa
      661 ccgggccccg acggatcggt gacccgaggc cggctttcgg cgatcaacct cgtcgccggc
      721 gtcgccctgc gtgtcgatct ggccgaagag gaagcgcgga tacttcgact ctgcaacggt
      781 gaccatacgg ccggcgagat cgccgacagc cgcgaactcc gccgccgctt cagccgcatc
      841 gggtccgaag gccgtcgaca gctgctgcgg cggttcctcc gccgcgccat cgaacacgag
      901 ttggtgtacc tatgtccgtg atcgcggagc tggccatgga ttcgcccgtc gccatcggcg
      961 gttccccgcc gcattggctg ccgcttgctg ccggcgaccg cctgggcgac cttgcgcgtc
     1021 actatgacga gtacccgcac cctccctatg ccgaccacgc gccgcaccga ggcgtgccgc
     1081 cacgagatct gctcgatctg gcgcggtatg tactgtggcc acggcgcaag gtgctggccg
     1141 acgcccgagt gctggatgcc ggctgcggca cgggcgcgca gctcgcggcc atgggcatgc
     1201 gtcacccgga tgtggagctc tggggcgtgg atgtgagcgc acggtcgctc gacgacgcgg
     1261 ccgaccgcgc ccggcgctgc aatgtcggct cggtccatct ccgccagctt cccgtcgagc
     1321 gagtggggga gctggggctc gagttcgacc tcgtggtact ccagggcgtc ctgtcggcga
     1381 gctcggaccc acgggccaca ctcagcgccg cggcctcggt ggttcgtccg gagggcggga
     1441 tactggtctc tgtggcggca ccctacggcc ggctcggtgc cgagggcttc cgcgaactgg
     1501 tcgacctcgc ccgaggcggg cagccgcgcg ccgagcggct gcggttcgcc cggaagctcc
     1561 gggccagccg agagtggcac tcgagactgc tcgaggccgt gctcgactcg cgcgaggagg
     1621 atgacgcgtc tctggccgaa ctggcggtgc tcgatccccc cttgcgagcg tactcggtgc
     1681 ctgagttaac cggtctgctg gcgtcggccg gcctgcggat ctggcgcttc catccgagga
     1741 cgccttacga gcccgagcgc tacctgccgg cgccccacca tgggtgcctc cgcgctgtgc
     1801 cggaagtgct tcgacctgcg gtcgccgagc tactccattc ggcgatgcac acccactgct
     1861 tcttcgcagt gcgggagggc ttcgaacccg aacgcgtgga cctggacggt gaggactggc
     1921 tcgatctaca gcccattgcc aacgggctat gggacttcgc agcctccgat gggcccgtgc
     1981 cggagcaggc tgggcgcgcc ggagtggcaa ctcgcgagtt ctgcgggcgg tgcgagtgca
     2041 ttgcgctcac gccgtggcag cggatcgctc tggagctgtg tgaatccggc acgcgtgtgc
     2101 gcgacattct gaggtccagg cggctccggc aggagttggc ggggctcggt cggggcgact
     2161 attgcgaggc ggtccggtca ttcctgcgcc gcgcggcgca cgacgaactc gtctacctgg
     2221 ttggctaggc cagaggcaag ggagaacaca gacatggcag tgacgccggt ggtcagcgcc
     2281 cctgggggcc tgagcgacgc ggtcctcgcc cagtatgagg cctatccgtg cccggggcac
     2341 ccacccggga cggcggagcc agaggtgccg gactggttgg gctatctccg tcccctgctg
     2401 tggccccgtc ggcaggatct atcggggctg aaggtgttgg acgccggttg tgggaccggt
     2461 gcgatgacct cggggctcgc ccgctgccat cccgaagtga acgtactggc catcgacatc
     2521 agtgcgcggt cgctcgaggt ggccgcccgg cgcctgggcg gctgccaggg cgggaatgtg
     2581 cgactccgtc agcttccgat cgagcgggcg aacgagctcg atgagcgctt tgacctgatc
     2641 gtggtctggg atgtgctgca ctgtgttatc gacgccccgc gagtcttgcg gacactgggt
     2701 gatctcttga cgcccgatgg cgcgctcatc gcgtcggtgc gggccccgca cgggcgcgcg
     2761 ggggcggtgg cgctccggtc tctcatggag gcactgggcc tggggctcga gtgccccgag
     2821 gcgatcccgc gggcgcgatc catcatcgac aatctctcgg ccactcacct gttccagcgg
     2881 tccgaatggc aacaggacct ggcagccggc ggggacgcgg cggtggtgga tctgctgctg
     2941 ccggcgcgtc agcggcttta caacgcgctc gaggtcttcg aactcgtggc cgagtcgggg
     3001 ctggagtttc tgcggtggtt ctgcccgaac atgtactgcc cacgccgcta cctggccgat
     3061 gc
//
LOCUS       DAOSPL010000442         3060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_32018_length_3060_cov_2.776705, whole genome shotgun sequence.
ACCESSION   DAOSPL010000442 DAOSPL010000000
VERSION     DAOSPL010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32018_length_3060_cov_2.776705"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PLQ54_09035"
     CDS             complement(<1..644)
                     /locus_tag="PLQ54_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93443.1"
                     /translation="MRRSACGVMLWALMAVGPSSTAAQANGLAAPVQLVAYKHGYGFV
                     LSEGRTRATDGWITTGQVPQASLGTFWLYSTDEGVRVDRAMTEENQGTESAAVQTYED
                     LLRANVGSEVKVRLTDGKAVEGTLVAVLPALPPATSPNDPVPSLVRPEYAYRYGYGPV
                     PVAPGGPVTHLVVQSGQETTTVSVGTVSTVQFGNEPPKEFARPSRQKKLSAHLLR"
     gene            765..2534
                     /locus_tag="PLQ54_09040"
     CDS             765..2534
                     /locus_tag="PLQ54_09040"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HOS93444.1"
                     /translation="MGLSAARRLGFQLIAAVLMLLAVAGAGWCVLPVSMLEPGMRGYG
                     KTVLAGTEVTTFDVRIVDVVRSNGRVPDLILIKVGGTAIEQAGGISAGMSGSPVYIRD
                     QLIGGIAYTTPFADTTYGYVAPIEYMLPLLDWPAEREDPWAVRGQLTGSLLDDLSRAN
                     SPVMVSGLSSRAFERLSDRLAGRGLRAVQGGAMGEFAGDQPLVPGSSVGIQLVRGDVN
                     VTAVGTMTHVDGDRFIAFGHSFIHQGPCQFPIVATKVAAVVPSTQLPFKMASPGTRVA
                     GTVTQDRQMGLGGRLGAAPSMVKLDLTVSGEHLAQPTPVTMDLVWDPELTAELVGTAS
                     LGAIDSCADREGVGSALVTLQVSLADGTSVTRADVVSTREDVGGYAALEAKDIVEALV
                     LNSIGPVLPKRISLGVHISRRPALARIAAVRVEPPTASPGDTVLVTAELVPFRAAAET
                     VLFSLRVPADAAPGFPRIRVHGKTAQSLLVYDDHETVTPTTLPEFLQMVEGSGKRRSL
                     VCDLVFSPFDRLPLSLPFSAFVPVQPEDAESLRDLPSRPPVLRDMATQGLLQLIRVGA
                     RVVQDTAYVIEGDVSVPLRIVAR"
     gene            2546..>3060
                     /locus_tag="PLQ54_09045"
     CDS             2546..>3060
                     /locus_tag="PLQ54_09045"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS93445.1"
                     /translation="MRDVFQLAPLVARVSAAVTHGIGFTGEAALLGWAAMGYAWRRRV
                     DWREVLRQLCVIGWSSLPAVLVTVFFSGAVAALHTADQFVQFGLSQVIGGAVAVSAAR
                     EIAPVLGAVIVLARAGAGMTAEIGSMKVTDQLDAMRSLGVSPTEYLVSPRLLAGMVAL
                     PGGKFKMGSDR"
BASE COUNT          403 a          987 c         1096 g          574 t
ORIGIN      
        1 cgcagcaggt gggccgagag cttcttctgc cgagatggcc gggcgaactc cttggggggc
       61 tcgttgccga actggacggt ggagacggtt cccaccgaga ccgtcgttgt ctcctggccc
      121 gattgaacga cgaggtgggt caccggtccg cccggggcca cagggactgg gccgtagccg
      181 tagcggtagg cgtactccgg tcgcaccagc gaggggacgg ggtcattcgg gctcgtcgcc
      241 ggcggcaggg ccggcaacac ggccaccaag gtgccctcga ccgccttgcc gtcggtcagt
      301 cggaccttga cctcagagcc gacattggcg cgcaacaggt cctcataggt ctgaacggcc
      361 gccgactcgg tgccctggtt ctcctccgtc atcgcgcggt cgacccggac gccctcgtcg
      421 gtcgaataga gccagaaggt gccgagcgag gcctggggga cttggccggt ggtgatccag
      481 ccgtcggtcg ccctagtgcg cccctcggac agcacgaacc cgtagccgtg cttgtaggcc
      541 acgagctgca ccggtgccgc caggccgttg gcctgggcag ccgtgcttga gggcccgacc
      601 gccatcaggg cccacagcat cacgccgcat gcactcctgc gcatcgtctt acttcctctc
      661 gccgagatgc catcggggcc cgagcgggcg tccgacccgc tgtagcccgg cacggcttct
      721 gccgctacac tgttcgaatg cctcggactt gggtggtgcg tcccatgggt ttgtctgccg
      781 ccaggcgtct cgggttccag ctgattgccg ctgtgctcat gttgctcgca gtcgctgggg
      841 ccggctggtg cgtcctcccc gtatcgatgc tcgagccggg gatgcgcggc tatgggaaga
      901 cggtgcttgc gggcactgag gtcacgacgt tcgacgtgcg cattgtcgat gtcgtccgta
      961 gcaacggccg ggtacctgac ctgatcctca tcaaagtggg cggaacggcc atcgagcagg
     1021 cgggcggcat ctcggcgggg atgagtggca gccccgtgta cattcgcgac cagctcatcg
     1081 gcgggatcgc ctacacgacg ccgttcgccg acacgaccta tggctatgtc gctccgatcg
     1141 agtacatgct tccgctactg gactggccgg ccgagcgcga ggatccctgg gcggtgcggg
     1201 ggcagctcac cgggtcgctc cttgacgatc tgtcccgtgc caacagcccg gtgatggtat
     1261 ccggtctttc gtcgcgggcc ttcgagcgcc tgagcgaccg tctggcgggc cgcgggcttc
     1321 gggcggtcca gggcggcgcc atgggggagt tcgcggggga ccagccgctg gtgccggggt
     1381 cgagcgtcgg catccagctc gtccgcggcg atgtgaacgt cactgccgtg ggtaccatga
     1441 cccacgtgga tggtgatcga ttcatcgcct ttggccactc gttcatccac cagggcccgt
     1501 gccagttccc tatcgtggct accaaggtgg cggccgtggt tccgagcacg cagctgccgt
     1561 tcaagatggc aagcccgggc acgcgggtgg ccggtacggt gacgcaggac cgccagatgg
     1621 ggctgggcgg gcggttgggc gccgcgccca gcatggtgaa gctcgacctg acggtctcgg
     1681 gcgagcatct ggcccaacct acgccggtga cgatggacct cgtgtgggat cccgaactca
     1741 cggccgagct cgtcggcacc gcctcgctgg gcgccatcga ctcgtgcgct gaccgggagg
     1801 gtgtgggttc ggctttggtg actctccagg tctcccttgc cgatggcaca tcggtgacgc
     1861 gggccgatgt ggtgtcgacg cgggaggatg tggggggcta tgccgcactc gaggcgaagg
     1921 atatcgtcga ggcccttgtc ctgaactcca tcggcccggt gctcccaaag cggattagcc
     1981 tcggtgtgca catctcgcgg cgtccggcgc tggcgcgcat cgcggcggtg agggttgagc
     2041 cccctacggc ctcgcctggc gacacggtgt tggtgacggc ggagctggtt ccctttcgag
     2101 cggccgccga gacggtcctg ttctcgctgc gcgtccctgc cgatgccgcg ccgggattcc
     2161 cgcggattcg tgtgcacggc aaaaccgccc agtcgcttct tgtctacgat gatcatgaga
     2221 cggtcacccc taccactctg cccgagttcc tacagatggt cgaggggagt ggcaagcgcc
     2281 gcagcctggt gtgcgacctg gttttctcgc ctttcgaccg gctgccgctg agcttgccat
     2341 tcagtgcctt cgtgcccgtg cagccggagg acgccgagtc gctgcgcgac ttgccatcgc
     2401 ggcccccggt gttgcgcgac atggcaacgc agggcctgct gcagctcatc cgcgttggtg
     2461 cgcgagtcgt gcaggacacg gcctacgtga tcgaggggga tgtttccgtt ccgttgcgca
     2521 tcgtggcccg atgaggggag gagccgtgcg tgacgtgttc caactggcgc cgttggtggc
     2581 gcgggtctcg gccgcagtga cccatggcat tgggttcacg ggcgaggccg ccctgctcgg
     2641 ctgggcagcc atgggatatg cctggcgccg gcgcgtcgac tggcgcgagg tgcttcgcca
     2701 gctgtgcgtg atcggatgga gttcgctccc cgcggtgctg gtgaccgtgt tcttctcggg
     2761 cgccgtggcg gcgcttcata ccgccgacca gttcgtgcag ttcggcctct cccaggtcat
     2821 cggtggcgcg gttgccgtgt cggcggcgcg cgagatcgcg ccggtgctcg gggcggtgat
     2881 cgttctggcc cgggccggcg cggggatgac ggccgagatc ggctcgatga aggtcaccga
     2941 ccaactcgat gctatgcggt ctctcggggt gagcccgacc gagtacctgg tgtcgccgcg
     3001 tctcctcgcc ggcatggtgg cgttgcccgg cggcaagttc aagatgggtt cggaccgctt
//
LOCUS       DAOSPL010000443         3059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_32035_length_3059_cov_2.900466, whole genome shotgun sequence.
ACCESSION   DAOSPL010000443 DAOSPL010000000
VERSION     DAOSPL010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32035_length_3059_cov_2.900466"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLQ54_09050"
     CDS             complement(<1..290)
                     /locus_tag="PLQ54_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93446.1"
                     /translation="MGTATSLTNAGSWGRPLRFWGTVTAGLLVVFGLSYAVGLGVGRR
                     LESQAVPVRQLKTWDAGKGFTLAERGEGLLERVFGPMGPDKAQVQIAVGEYLT"
     gene            complement(311..1492)
                     /locus_tag="PLQ54_09055"
     CDS             complement(311..1492)
                     /locus_tag="PLQ54_09055"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOS93447.1"
                     /translation="MRLSTRATNVSPSATLAISRKAKELRSKGIDVIGFGAGEPDFDT
                     PAHIAAAAALAVTSGDTRYAPSKLPELHSAVCEAVREATGVSYTPKQVLVSCGAKHTL
                     YNVMQALIDPGDEVILFAPYWVTYPEQVALAGGRPVIVETSVEDDFAPDLEAVRAAIT
                     GRTRAIIVNSPSNPCGSVYGESVLEGLAAIAVEHDLMVVSDEIYDRLTYDGRPHRSIL
                     ECGAGMAERTVYVNGPSKTYAMTGWRLGWALGPEPLISAMADLQDHCTSNPASFAIAG
                     CVEALTGPQDCVEAMRAEFEQRRDFMYGRLSSMSCCRVSKPRGAFYALPDFSPIMGTT
                     RAGVTIQDSMDLAGFLLEHAGVALVPGTPFGAPTSARLSFATSMAHIEEGLDRIERAL
                     C"
     gene            1655..>3059
                     /locus_tag="PLQ54_09060"
     CDS             1655..>3059
                     /locus_tag="PLQ54_09060"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOS93448.1"
                     /translation="MVARVLALFLCGCAAVAAPTELRGVWSHATAMDTPEKAAALLAR
                     LEDARLNAIYPLCFYWGGTAYWSSRIAPQAEVVRRGFDPVAHWLSIARPRGIATHVRL
                     VVGNYGTSEAGALFPAHPEWRLVDVSGRPGNWYDLGNPEVREFMVRLATEVAATYAVD
                     GVQLDFVRYPGHEWGYTAAAKRGFRETTGYDLDPLLGSSLPSIICASSNRVGPVSTAR
                     PLAVFDNGHPAVLVNALGSGTAAVLNWQAFASRTPASDEILKRIATSFGASPATLRYT
                     YPAQTRARYGQRAVDQMQAWLRSVLGYAAQPVDAQRLEELSRRCLLLLPGCYLMDNTA
                     ANGLELFVRGGGRAIFVDGPAPTMGNAHLRAVTGAQGGLGWSSTSAVLLPTSPDPRIP
                     SRGAPMSPEEAGALIRAWDTYRESCITDLVRQTAARVHEARPGAQVTAAVFRSRASAA
                     ERLQDWPRWLREGLVDGV"
BASE COUNT          499 a         1104 c          969 g          487 t
ORIGIN      
        1 gtcagatact ctcccaccgc aatctgcact tgcgccttat cagggcccat cggcccgaag
       61 acgcgctcga gcagcccctc gcctcgctcg gccaacgtga accccttgcc ggcgtcccag
      121 gtcttgagtt gccgcaccgg cacggcctga gactcgagcc gccgccccac gcccaggccc
      181 acggcgtacg acaggccgaa cacaaccagc aagccagcag tgacggtccc ccagaacctc
      241 aagggacgtc cccacgagcc agcgttcgtc agcgacgtcg cagtgcccat gcatcctcca
      301 ttcgcgaaac ctaacacagg gctcgctcga tgcggtccag gccctcctcg atgtgagcca
      361 tggacgtcgc gaacgacagc cgcgcagagg tcggcgcccc gaacggcgtg cccggcacca
      421 acgcaactcc agcgtgctcg agcagaaagc cggccaggtc catagagtct tggatggtca
      481 cgcccgcccg ggtggttccc atgatgggcg agaagtcggg aagtgcgtag aacgcacccc
      541 gcggcttcga gacccggcag cagctcatcg aggacagacg accgtacatg aaatccctgc
      601 gctgctcgaa ctcagcgcgc atggcctcga cgcagtcctg tgggcccgtg agtgcctcca
      661 cgcagcctgc gatggcgaac gaggccggat tcgacgtgca gtgatcctga aggtcggcca
      721 tggcgctgat caatggctct gggcccagcg cccaccccag ccgccatccc gtcatcgcgt
      781 acgtcttcga cgggccattg acgtacaccg tgcgctcagc catccccgcc ccacactcaa
      841 ggatgctgcg gtgcggccgg ccatcgtagg taagtcgatc atagatctcg tcggaaacga
      901 ccatcagatc gtgctcaacg gcaatcgccg ccaggccctc cagcacggac tcaccataca
      961 cgctgccaca gggattgctc gggctattga cgatgatggc ccgggtgcga ccggtaatcg
     1021 cggcgcggac cgcctccagg tccggcgcga aatcatcctc gacggacgtc tctacgatca
     1081 ctggtcttcc gccggccagc gcgacctgtt caggataagt cacccagtag ggcgcaaaca
     1141 ggatgacctc atcgcccggg tcgatcaggg cctgcatgac attgtagaga gtatgttttg
     1201 ctccacacga aaccaacacc tgcttcggcg tgtagctcac gcccgtggcc tcgcggacgg
     1261 cctcgcagac ggcgctgtgc agctccggga gcttgctcgg ggcataccgg gtgtcaccgc
     1321 tcgtcacggc gagggccgct gccgctgcga tgtgagccgg cgtgtcaaag tcaggctccc
     1381 ccgccccgaa accaataacg tcgatgccct tcgaccgcag ctccttcgct ttgcggctga
     1441 tcgccagggt cgccgacggc gacacgttgg tggcgcgggt ggacagtctc atggcacgat
     1501 cctccactcg ggccgacctc gcgcccggca ggacgcggtg gccacgaacc atacggtggc
     1561 tagcgtagcc gagggcgccc gcaccgtcaa cgctctccag ccgccgttga cgcctctggg
     1621 cgggcgtcca cataatgccc gcatgtgggg acccatggtc gcgagggtgc ttgcactatt
     1681 cctgtgcggc tgcgccgctg tggccgcgcc gacagagctt cgtggcgtct ggtcccacgc
     1741 caccgcgatg gacacgccgg agaaggcggc ggccctactc gccaggctcg aggacgcccg
     1801 cctcaatgcc atataccccc tgtgcttcta ttggggcggg acggcctact ggtcgagccg
     1861 catcgcaccg caggccgagg tcgtgcgccg gggattcgac ccggtggccc actggctgtc
     1921 catcgcgagg ccccggggca tcgccaccca cgtccggttg gtcgttggga actacggtac
     1981 gagtgaggca ggtgcgctgt tcccggccca tccggagtgg cgcctcgtcg acgtctcggg
     2041 ccgccccggc aactggtatg acctgggcaa ccccgaggtg cgcgagttca tggtccgact
     2101 ggcaaccgag gtcgccgcga cctacgccgt cgatggcgtc cagcttgact tcgtccgcta
     2161 cccgggccac gagtggggat acacggctgc cgccaagcgc ggcttccgag agacaaccgg
     2221 gtacgacctc gatcccctcc tcggctcctc gcttccctcc atcatctgcg ccagctcgaa
     2281 ccgcgtcggt ccggtgagca ccgcacggcc gctggcggtg ttcgacaacg ggcacccggc
     2341 cgtgttggtc aacgcgctgg gctccgggac ggccgccgtc ctgaactggc aggcgttcgc
     2401 ctcgcggacc cccgccagtg atgagatcct caaaaggatt gcgacgagct tcggcgcgag
     2461 cccggcaacg ctccgctaca cctatcccgc ccagacgcga gcccgctacg ggcagcgtgc
     2521 ggtggaccag atgcaggcgt ggctccggtc ggtcctgggc tatgcagcgc agcccgttga
     2581 cgcccaacgc ctcgaggagc tcagccgcag gtgccttctc ctgcttcccg ggtgttacct
     2641 catggataac accgccgcca acggcctcga gctgttcgtt cgaggcggcg gcagggccat
     2701 attcgtcgac ggcccggcac ccaccatggg caacgcgcat ctccgggctg tcaccggcgc
     2761 ccaggggggc ctgggctggt ccagcaccag cgccgtgctc cttcctacca gcccggatcc
     2821 tcggatcccg tcgcgcggcg cgcccatgtc gcccgaagag gctggcgccc tgatccgggc
     2881 ttgggatacg taccgcgaaa gctgtatcac cgacctcgtg cgccagacgg ccgctcgggt
     2941 gcacgaagcg cgcccgggcg ctcaggtgac ggcggcggtg ttccgctcgc gtgcctcggc
     3001 ggccgagcgc cttcaggact ggccccgctg gctccgcgag ggcttggtcg atggcgtct
//
LOCUS       DAOSPL010000444         3055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_32081_length_3055_cov_3.601000, whole genome shotgun sequence.
ACCESSION   DAOSPL010000444 DAOSPL010000000
VERSION     DAOSPL010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32081_length_3055_cov_3.601000"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..603
                     /locus_tag="PLQ54_09065"
     CDS             118..603
                     /locus_tag="PLQ54_09065"
                     /inference="COORDINATES: protein motif:HMM:NF014053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS93449.1"
                     /translation="MSLFAPVTEKSITRGIVERFTRELLSVTDSDVIIVGAGPSGLMA
                     GRDLARRGARTVIFESNNYLGGGFWIGGYLMNQLTIRDPGHRVLDELGVPYEAYEPGL
                     YTAFAPHACSKLIAATCDAGCKVISMTSVDDVVLRDGPLLLMEPEPTRAARNTTGPLS
                     R"
     gene            complement(727..1446)
                     /locus_tag="PLQ54_09070"
     CDS             complement(727..1446)
                     /locus_tag="PLQ54_09070"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOS93450.1"
                     /translation="MDSPLPSRRVNIEQLVDRRAKHWDHARETQAGLQAVRRTGLGPY
                     VAISRAIGTHGRTISQLVADLLGWDYYEREIVEFIAKRAQVREHMVKSLGETQREEAM
                     NWVRSMIDGHAVAETVYFRHLVAVITGIAAHGNAVILGRGAQFILPPSAGLRVRLYAP
                     KPWRIETAMVRYNTTREEAEKLIRDSDRAQARFYKDRFHKDMLDPLGYDLMVNVSQMD
                     VEGAARSIVCALRGKLGSEAL"
     gene            1712..2806
                     /locus_tag="PLQ54_09075"
     CDS             1712..2806
                     /locus_tag="PLQ54_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93451.1"
                     /translation="MANSALRCPDPLQAQPCSLLAADASASCCRPDGAAGPDLFWIGT
                     GPSAGMPPSHRPFTAAVVVTAPRVGGRLERDAIATIDRLRRALSAGEPLHGPKLDLRE
                     RLRLAFVASQPPRGCPLMACIVSGKHVAFAWQGDCTAYVIRDASPSAAYEVLQGPSAG
                     RPSASLWGAPAAAPHTHELLAAPGDVLVLTAGGIGQVLLGPADASRRIAGCETLVDAC
                     SRLLALRPSTAPVRARTAALVQMRFNLDARPATAPNAEPQLVALAGGAPREGGRPGRH
                     RSELRRHRGRRPTGWRAVEVGLLTAALCASTALIWWQHEAGWPARDYLSQVASFTASQ
                     VPDATISGAPELLLEFNDGLLGGFLSDSAF"
BASE COUNT          485 a         1077 c          990 g          503 t
ORIGIN      
        1 agccggacgg cgacgcaata aagttgatgt attaccctaa ctagcctatc attttgcggc
       61 gccccggtgg tccgggcgta ggcgggtgcc gcatctctct ttccgcggag gtaccccatg
      121 agcctgttcg ctccagtaac cgagaagagc atcacccggg gcatcgtcga gcggttcact
      181 cgcgagctcc tgtccgtcac cgacagcgac gtcatcatcg tcggtgccgg acccagtggc
      241 ctgatggccg gccgcgacct ggcacgccgt ggcgcgcgca ccgtcatctt cgagagcaac
      301 aactacctgg gaggcgggtt ctggatcggc ggctacctga tgaaccaact gaccatccgc
      361 gaccccggcc accgggtgct ggacgagctc ggggtcccct acgaagcgta tgagcctggc
      421 ctgtacaccg ccttcgcacc ccatgcgtgc tcgaagctca tcgcggccac ctgcgatgcc
      481 gggtgcaagg tcatcagtat gaccagtgtg gatgacgtgg tgctccgtga cgggccgcta
      541 ctcctgatgg agccagaacc aacgcgggcg gcccggaaca ccaccgggcc gctgtctcga
      601 tgactccgct gcctgcgggc taggtccaca ggctgcaaga gcagcaggcc gggcactcgg
      661 tccggggcaa cagcgacgcc agcctgcttc cggaagacat cccccagtgc ctcacgcgga
      721 gtggcatcaa agagcttcag acccaagctt cccacgaagg gcgcaaacga tcgaccgcgc
      781 ggcaccttcc acatccattt gtgatacatt caccataaga tcgtagccga gagggtcgag
      841 catgtctttg tggaagcgat ccttgtagaa cctcgcttgc gcccgatcgg agtctctgat
      901 gagcttctcg gcttcctccc gagtcgtgtt gtaacgaacc atggccgtct cgatccgcca
      961 cggcttgggg gcgtatagcc gcacccggag tcccgcggat ggaggaagga tgaactgggc
     1021 gccccgcccc aggattacgg cgtttccatg ggccgcaatc cccgtgatga ccgcaaccaa
     1081 gtgccggaag tagaccgtct ctgccaccgc atgaccatcg atcatgctcc ggacccagtt
     1141 catggcctcc tcccgctgcg tctccccgag agacttcacc atgtgctcgc ggacctgagc
     1201 ccgtttggcg atgaactcga caatctcccg ctcatagtag tcccagccca gaaggtcggc
     1261 caccagctgg gagatggtgc ggccgtgggt gccgatggcg cgcgagatgg ccacataggg
     1321 ccccagaccc gtgcgccgga cagcctggag acccgcctgg gtctcgcggg catggtccca
     1381 gtgcttggcg cggcgatcca cgagctgctc gatgttgacc cgtctgctcg gcagaggcga
     1441 atccatggga acacctcccg aggatgcctt acacccatgt tcctaactac gccgtccgga
     1501 aacgaacacc tccgcacaac tggtgtgagc gcgcgagtcc ggcttggggc gtaacttcac
     1561 ctcgctttga gcccgagcgc ccgcgaaggc gttcggggta cacagaggcc gtgccgcggc
     1621 acgccctccc cctggttgcc ctggcgaaag accgttccgt cgagcacttg cgctgaggcg
     1681 ctggaagccg gcggcaaggc agggacctat catggccaac tcggcgcttc ggtgcccgga
     1741 cccccttcag gcccagccct gtagcctgtt ggcggccgat gcatccgcat cgtgctgtcg
     1801 tccggacggg gctgcagggc ccgacctgtt ctggatcggc acgggcccga gcgcgggcat
     1861 gcctccgtcg caccgtccct ttacggcggc cgtggtcgtg accgcgcctc gggtgggcgg
     1921 gcgcctggag cgcgatgcca tcgccaccat cgaccgcctc cgccgggccc tctcagcggg
     1981 agaacccctg cacggcccca aactcgatct gcgggagcga ctgcgcctcg cgttcgtcgc
     2041 cagccagccg ccccgcggtt gcccgctcat ggcatgcatc gtcagcggta agcatgtggc
     2101 cttcgcctgg cagggcgact gcacggccta tgtcatccgc gatgcatcgc cttcggccgc
     2161 gtacgaggtg ctccagggcc cgtcggccgg acgcccctcg gccagtctct ggggcgcgcc
     2221 cgcagcggcg ccgcacaccc atgagctgtt ggccgcgccc ggcgatgtgc tggtgctgac
     2281 ggccggcggc atcggtcagg ttctcctcgg acccgccgac gccagccggc ggatcgctgg
     2341 gtgcgagaca ctggttgacg cctgctcgcg cctcctggca ttgcggccct cgaccgcacc
     2401 ggtccgggct cggaccgcgg ccctggtgca gatgcggttc aacctcgacg ctcgcccggc
     2461 cacggcgccg aatgccgagc cccaactcgt cgcactggcg ggcggggcac cccgggaggg
     2521 cggccgcccc gggcgacacc gcagcgagct gcgcagacac cgcggacggc gacccaccgg
     2581 gtggagagcc gtcgaggtcg gactcctgac cgccgccctc tgcgcgtcga ctgccctcat
     2641 atggtggcag cacgaagctg gctggcctgc ccgggactac ctcagtcaag tggcctcatt
     2701 cacggcgagc caggtgcccg acgccaccat cagtggcgcc cccgagcttc tgctcgagtt
     2761 caacgatggg ctgctcgggg gattcctgtc cgactcggcg ttctagcgac cgctccgtcg
     2821 tgggactgag ggcacaagag gggcctgcct ggtgcaggca tggtcccgtc atcgaaggac
     2881 ccacagcaca cggccgcggc ccatcgggct cccgggttgc gccctgacgc gccaaacagt
     2941 gcgatgagaa gccggtcgcg gcatcgcagg ccaacggttg gcctcgggtg ttggggggca
     3001 ctgacggcga atgccaccgt ggctttcgtc gacgcgcgcg ctttcgcccc gcgtg
//
LOCUS       DAOSPL010000445         3052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_32141_length_3052_cov_3.119453, whole genome shotgun sequence.
ACCESSION   DAOSPL010000445 DAOSPL010000000
VERSION     DAOSPL010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32141_length_3052_cov_3.119453"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            251..1243
                     /gene="rsmH"
                     /locus_tag="PLQ54_09080"
     CDS             251..1243
                     /gene="rsmH"
                     /locus_tag="PLQ54_09080"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOS93452.1"
                     /translation="MIGPCDHDYTPHDPVMMAEVLALLEPRPGQVVLDATAGSGAATA
                     ALLEAVSPGGRVVALDRDPEAVAHCRARFGHTAGRVLVIHANFGAVGEVLHGTEAGGF
                     DQALFDLGVSAHQLSGRANRGFAFSHDSPLDMRMDPRAPLSARDLLASLSEQELSALI
                     RDLGEDRHHARIARAICRARQEHAIETTAQLARIISDAVPSGPPQRIHPATRTFMALR
                     MHLNNELGELSAALEALGRIMRPGGRVAFLAYHSLEDRMVKEYFRTAQRGCICPPRVP
                     ACGCGRSPSLRVLTPKPVRPTEAEVRRNPRARSARLRVAEWIGGTAGYAQGRQP"
     gene            1240..1695
                     /locus_tag="PLQ54_09085"
     CDS             1240..1695
                     /locus_tag="PLQ54_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93453.1"
                     /translation="MRRERFEGSARVQWERAVAYRRWMVVVVVLTLVCLARVGLGAHL
                     TQLGRRTQALEKRHAELAERHARLTTELSRKSSTASVRERLAAAGITVVAGGSDCTPL
                     RLPVVAQTACSSPDLASRVSWAIADAGRAAWPEAPRPVTPRAVAWARGR"
     gene            1762..3048
                     /locus_tag="PLQ54_09090"
     CDS             1762..3048
                     /locus_tag="PLQ54_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOS93454.1"
                     /translation="MDAVHVLIVLGFVLVVGRLFQVQVLQHEAWVRAVHDYVWASVTK
                     PAHRGSIYDRSGIELAVDQVSYSIGIDPKVIRTQGTDLAAVAMSTGLDLGVLRRRAAA
                     NTRYEYVARDVSEETAKRVREAAKVHAGLNVEEKATRLYPQHSLAAALIGVAGADRVG
                     LSGIERSMNRPLAGVAGRSRVGLDPKGQPIPGTEEVITPVVHGDSLWLTLDAGIQAAC
                     ERELAKCMKQHHAQRATALVIAPQTGAIVAAASLPGFDPNCIRPGEVSRMTMLFSQFV
                     FEPGSTMKPLVLAEAIDRGIIGPSEVFRCRGAERVGPDEIHCDHPPRGGHGAVDAQAI
                     LRVSCNLGAMHVGLRMKNDRMIQALRAFGLLSPTGVEVDEQVGSTPRRETLWPSWCGT
                     AAFGQSVAVTPLGLAMGYACLANDGSSCVRTWLRSE"
BASE COUNT          446 a          960 c         1160 g          486 t
ORIGIN      
        1 tggatcgcgc ttatgtccgc cggcacctgg aggaagcgct ggccgacgag gacattgggc
       61 ggttcatctt gtaggacgca agccggccca tccgatcgag cttgcgtgtg cccgcggtga
      121 gtggccggcg cccctggtca ctctcggtgc acatggcggc cggggtagcc ggggcccagc
      181 gcgcgggcag tccggctgcg gagccaagcc ggcccgacca ctggcgcaac gccaaggagc
      241 cgcagcgggc atgattgggc cctgtgatca cgactacacc ccgcatgacc ccgtgatgat
      301 ggcggaggtg ctggccctac tggagccccg gccgggccag gtggtactgg acgccacggc
      361 gggcagtggg gccgcgaccg cggcattgct tgaggcggtc agccccggcg ggcgcgtggt
      421 agctctggac cgggatcccg aagcggtcgc ccattgccgg gcgaggttcg ggcacactgc
      481 cggccgggtt ctcgtgatcc acgcgaactt cggcgccgtc ggcgaggttc tacatggaac
      541 cgaggccggt ggattcgacc aggcgctgtt cgacctgggc gtttcggcac atcagctgtc
      601 cgggcgcgcg aaccgagggt tcgccttctc ccacgactcg ccgctggaca tgcggatgga
      661 cccgagggcc cccctgagcg cccgggacct actggcctcg ctgtcggaac aggagttgtc
      721 ggccctgatc cgggatctgg gagaagatcg gcatcatgct cggatcgccc gtgccatctg
      781 ccgggcgagg caggagcacg cgatcgagac cacggcgcag ctggcccgga tcatatccga
      841 cgctgtccct tcggggccgc cgcagaggat ccatccggcg acgcggacat tcatggcgct
      901 gcgcatgcac ctgaacaacg agctcggcga gctctcggcg gcgctggagg ccctgggacg
      961 catcatgcgc ccgggaggac gcgtggcctt cctcgcgtac cactcgttgg aggaccggat
     1021 ggtgaaggag tacttccgga cggcgcagcg aggctgcatc tgcccgcccc gagtccccgc
     1081 gtgcgggtgc gggcggagcc cgtctctgag ggtactgacg cccaagccgg ttcgtccgac
     1141 ggaagcagag gtccgtcgca acccacgggc ccgaagcgcg cggctgagag tcgcggagtg
     1201 gatcggcggc acggctggct acgcccaagg gaggcagcca tgaggcggga gcggttcgag
     1261 ggatcagcgc gcgtccagtg ggagcgtgcc gtggcatatc gccgctggat ggtggtggtg
     1321 gtggtattga ctttggtctg cctcgcccgg gtcgggctgg gtgcacacct gacgcagttg
     1381 gggcggcgga cccaggctct cgagaagcgg catgccgaac tggccgaacg ccatgcgcgg
     1441 ctgaccacgg agctaagtcg caagagctcg accgcgagcg tgcgggagcg gctggcggcc
     1501 gccggcatca ccgtggtggc cgggggaagc gactgcacgc cacttcggct gccggtggtg
     1561 gcgcagacgg cgtgctcgag cccggacctg gcgtcacgcg ttagctgggc catcgcagac
     1621 gccggccgcg cggcgtggcc cgaggctccg cggccagtca cgccgcgagc ggtggcctgg
     1681 gctcgaggac ggtaggccat gggcgcccgc gctcacactc ggctcccgag cggcccgtcg
     1741 ccatggctgg agacctggcg agtggatgcg gtgcacgttc tgatcgttct ggggttcgtc
     1801 ctggtcgtgg gtcggctctt ccaggtgcag gttctccagc atgaggcgtg ggtgcgggcg
     1861 gtccacgact atgtgtgggc ttcggtgacg aagccggccc accgcggttc catctacgat
     1921 cgctcgggga tcgagttggc cgtcgaccag gtgtcgtact cgatcggtat cgatccgaag
     1981 gtcatccgga cgcagggcac ggacctggcg gcggtggcca tgagcacagg gctggacctt
     2041 ggcgtgctgc ggcgccgggc ggctgcgaac acgcgctatg agtatgtggc ccgggatgtc
     2101 tccgaggaga cggcgaagcg ggttcgggag gcggcgaagg tccatgccgg gctcaacgtg
     2161 gaggagaagg cgacacgcct ctacccgcag cactcgctgg ccgcagcgct gatcggcgtg
     2221 gcgggggcgg accgcgtggg gttgtccggg attgagcgat cgatgaaccg tcctctggcc
     2281 ggcgtggctg gcaggtcgcg ggtggggctt gaccccaagg gtcagccgat cccgggcacg
     2341 gaggaggtca tcacaccggt cgttcacggt gactcgttgt ggctcactct cgacgcgggc
     2401 atccaggcgg cctgcgagcg cgagctggcc aagtgcatga aacagcacca tgcgcagcgg
     2461 gccacggcgc tggtcattgc cccgcagacg ggggccatcg tggcggcggc ttcgctgccc
     2521 ggcttcgacc cgaattgcat cagaccgggc gaggtcagtc gcatgaccat gctgttctcg
     2581 cagtttgtgt tcgagcccgg gtcgacgatg aagccgctgg tgctggcgga ggccatagac
     2641 cggggtatca tcggcccgag cgaggtgttc cggtgccggg gtgcggagcg cgtgggcccc
     2701 gacgagattc actgcgacca ccccccccgg ggagggcacg gtgccgtcga cgcgcaggcg
     2761 attctccggg tctcgtgcaa tctgggcgcc atgcacgtgg gtctccgcat gaagaacgat
     2821 cgcatgatcc aggcgcttcg ggcctttggt ttgctgtcgc cgacgggagt ggaggtcgac
     2881 gagcaggtcg gctcgactcc gcggcgagag acactgtggc cgagctggtg tggcacggct
     2941 gccttcggtc agtcggttgc cgtgacacca ctgggcctcg ccatggggta cgcgtgcctg
     3001 gcgaacgacg gcagctcatg cgtccgcact tggttgcgaa gcgagtgaca cc
//
LOCUS       DAOSPL010000446         3052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_32146_length_3052_cov_2.903904, whole genome shotgun sequence.
ACCESSION   DAOSPL010000446 DAOSPL010000000
VERSION     DAOSPL010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32146_length_3052_cov_2.903904"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..994)
                     /locus_tag="PLQ54_09095"
     CDS             complement(38..994)
                     /locus_tag="PLQ54_09095"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOS93455.1"
                     /translation="MDDGRANLLIFSGNGCRQLAERVAQHIGTKVGDAVVSTWTNGEI
                     RVQLREHVRGRDVFIFQSFGRNVNDRVMELLVMLDAARRASAARITVVAPYFPYSKQE
                     KKFRGREPISARLVADLLTSAGATRLLTMDLHEGAIQGFFNIPVDHLPSIPYVSRFLA
                     DRGLCGPDLVVVAPDEGAVEEAVLLSGLLGCGIAVVFKRHMEAAPEAVQTVEVVGDID
                     GKRALIWDDMILGGSTLVNAADMALERGARSVHACITHPVLCGDAVKRIEESSLAELI
                     VTDSIPLPEGLESTKITTISISSLLGDAIARISRNDSVSELL"
     gene            1205..2176
                     /locus_tag="PLQ54_09100"
     CDS             1205..2176
                     /locus_tag="PLQ54_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS93456.1"
                     /translation="MSVMPRERAIAALELRPPYPGLVPAMELEFQLTLERFGRQYHSA
                     AEYAEAASQSELSRLTREDAQLFVDTCRAYDLCGVLLPNHTGPPGVPIEERTRQLVGA
                     IRDISGDEFLMMCHGDATLSLPDGNAMWDLSMRMAEDKDALLAELGRWVDGALDGGER
                     LLAAGIEAFALCADYCYNSGPWCSPRMFAEFVTPFLARLTQGYRDMGAYVIKHTDGNI
                     MPILDQLVSTRPHALHSLDPQGGVDIAEVKRICGDQVCLIGNVNCGLVQTGTEDEVIA
                     SARYALDHGMPGGGFIFALSNVAFRGMPLERYELVQRVRHDHGIYAG"
     gene            2310..>3052
                     /locus_tag="PLQ54_09105"
     CDS             2310..>3052
                     /locus_tag="PLQ54_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93457.1"
                     /translation="MSTTNNRLGLVEAALADGGGILRCDPAWVARSFLPTGFRLGLSE
                     EEATVGERGFISERWLCSTTRADNEIGPPDEGLSYLRLDGGARMTLKEAVEIAGPFIL
                     GEEYAATHGHTLGRLAKIYDFGARIPYHIHQRACDAAKVGRHPKEEAYYFPEGIDLGP
                     HPETFFGVHPYIVREGKQELLLPYLVEWNSDLILRHSRAYLLVPGEGFHLPAGVLHAP
                     GTAVTIELQEDSDVFAFLQAKVHGKIVSK"
BASE COUNT          537 a         1034 c          956 g          525 t
ORIGIN      
        1 cggccctcgc caagtccggg cccacgcccg cccggctcta caggagctca ctcaccgagt
       61 cgttgcggct aatccgagcg atcgcgtcac caagtaggct actgatggat atggtggtga
      121 tcttcgtcga ctccagaccc tctggcaggg ggatggagtc ggtcacgatc agttcggcga
      181 ggctggactc ctcgattcgc ttcaccgcat cgccgcacag cacgggatgc gtgatacacg
      241 cgtgcaccga ccgcgcgccc cgctccaacg ccatgtcagc cgcattcacc agggtcgagc
      301 cacccaagat catatcgtcc cagatcaggg cacgcttgcc gtcgatgtcg cccacgacct
      361 cgacggtctg gaccgcctca ggagcggcct ccatgtggcg cttgaacacc acggcgatcc
      421 cacacccgag caagcccgac aggagcaccg cttcctcgac ggccccttcg tctggtgcca
      481 cgacgacgag atccggaccg cacagccccc ggtccgccag gaaacgcgag acatatggga
      541 tcgatggcag atggtcaacg ggaatgttga agaaaccttg aatagcaccc tcgtgcaagt
      601 ccatggtcag cagccgagtc gcccccgccg atgtgagcag gtcagccacc aggcgggccg
      661 agatgggttc ccgtccacgg aacttcttct cctgcttcga gtacgggaag tacggcgcga
      721 ccacggtgat gcgcgccgcc gacgcacgtc gggcggcgtc cagcatgacg agcagctcca
      781 tcacacggtc gttcacgtta cgtccgaagg actggaagat gaacacatcg cgcccccgaa
      841 cgtgctcgcg gagctggacg cgtatctccc cgttggtcca ggtcgagacc acggcgtcgc
      901 ccaccttggt cccgatatgc tgcgcgaccc gctccgccag ctgccggcat ccgttgccgg
      961 agaagatgag caagttcgcg cgcccgtcgt ccatcgtggt gccccctcag tcggtcgtac
     1021 aacctctcgg aagcgcattc gccccggggg tcgggctagt ataccacaac cggggccaag
     1081 cgacggcccg tcgaccatgc aacgttctcg ccatgttccc acgcaggagg gagtggtcgc
     1141 tgcgcccggc aatataccgt ccagtctggc caaccataac attcccccgg acggagtggg
     1201 ccccatgtcc gttatgccac gcgaacgcgc catcgccgct ctcgagctcc gcccgcccta
     1261 tccggggctt gtgcccgcca tggagctcga gtttcagctc accctcgagc ggttcggacg
     1321 gcagtaccat tcggccgccg agtatgccga ggccgcatcg caaagtgagc tcagccggct
     1381 cacccgcgag gacgcccagc tgttcgtcga cacctgccgg gcctatgatc tctgtggcgt
     1441 gctgctgccc aaccatacgg gaccgcccgg ggtgccgatc gaggagcgca ccaggcaact
     1501 ggttggcgcc attcgcgaca tctcgggcga cgagttcctc atgatgtgcc acggtgacgc
     1561 gacgctgtct cttccggacg gcaacgccat gtgggacctt tcgatgcgca tggccgagga
     1621 caaggacgcc ctgctggccg agttgggccg atgggtcgat ggcgcgctgg acggcggcga
     1681 gaggctcttg gccgcgggta ttgaggcgtt cgcgctctgt gccgactact gctacaacag
     1741 cggcccctgg tgctctcccc gcatgttcgc ggagttcgta accccttttc tcgcccgctt
     1801 gacccagggc tatcgtgaca tgggagccta tgtcatcaag cacaccgacg gtaacatcat
     1861 gcccatcttg gaccagctcg tctccacgcg gccgcacgca ctccattcgc tggacccgca
     1921 gggcggtgtc gacatcgcgg aagtcaagcg catctgcggt gaccaggtct gcctcatcgg
     1981 caatgtgaac tgcgggcttg tgcagaccgg caccgaggac gaagtcatcg ccagtgcccg
     2041 atatgccctc gaccacggga tgccgggcgg cgggttcatc ttcgcgctca gcaacgtggc
     2101 cttccgcggc atgccgctgg agcggtatga gctggtccag cgtgtgcgcc acgatcacgg
     2161 catctatgcc ggctagctcg tggcacagga aggggcgcgt gccgcggttg gcgcacactc
     2221 ggtgggggga gctacagcgc ggagcttcag gccggctcgc gccggggacg gcgggtgccc
     2281 ggcccatgtg attgaggaag tgattgatta tgtcaacaac aaacaacagg ctgggactcg
     2341 tggaggcggc tctcgcagac gggggtggta tcctccgctg cgatccggct tgggtggccc
     2401 gcagcttcct gcctaccggc ttccgtctgg gcctcagcga ggaagaggcc accgtaggcg
     2461 aacggggctt catctctgag cggtggctgt gctcgaccac cagagccgac aacgagatcg
     2521 gcccgccgga cgagggcctg agctacctcc ggctcgatgg cggcgcccgc atgactctca
     2581 aagaggccgt cgagattgct ggtccattca ttctcggcga ggagtacgcg gcaactcatg
     2641 ggcacacgct ggggcgcctg gcgaagatct acgacttcgg cgcccgcatc ccctaccaca
     2701 tccaccagcg agcgtgcgac gcggccaaag tcggacggca ccccaaagag gaagcgtact
     2761 acttccccga gggcatcgac ctgggccccc accctgagac gttcttcggc gtgcatccct
     2821 acatcgttcg cgagggcaag caggagctgc tgctgcccta cctggttgag tggaacagcg
     2881 acctgatcct ccggcactcg cgcgcctact tgctggtgcc cggagagggc ttccatctgc
     2941 ccgccggcgt cctccatgct cccggaactg cggtcacgat cgagctccag gaggactcgg
     3001 atgtgtttgc cttccttcag gccaaagtgc acgggaagat cgtgtccaag ga
//
LOCUS       DAOSPL010000447         3052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_32147_length_3052_cov_2.643644, whole genome shotgun sequence.
ACCESSION   DAOSPL010000447 DAOSPL010000000
VERSION     DAOSPL010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32147_length_3052_cov_2.643644"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2199)
                     /locus_tag="PLQ54_09110"
     CDS             complement(<1..2199)
                     /locus_tag="PLQ54_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93458.1"
                     /translation="MSRLSARSPLRRWRAFRPLPMAVVALAWAGAAWGQPGVAELGDP
                     GATSFDVVIAVPNPAALEGRVVLAGGVNALEVAFAGGRATIGETKGGPPRDLASGALP
                     ATEAPASVVVHRDDALIRVVAGPATVCEAYSRAVSQCVVQYDLPGLPEGAAMVQPLGD
                     ITFSEDFTWENRSQAAEAWERTLGSWTTAKAGDWTNPEAIRRQQAQTSTNLFAYVGHG
                     EPMALSVTGYPHWTNYEVQTGAKSLTGATVGLVAYYTGPNDYLVFRATSQTSGQDDAG
                     RYQLVLVRDGQPSILAEAPGGLVTNQWYKLRLRLHDGAVRADVDGVNVLAVDGLTALS
                     RGKIGLCADGPGGAKFDDVSVRSYRVFVDDYASGVTAQWDAPADGWQVAKVGDSRGHV
                     LVRKSAETGTALAGDETWSDYSVRADVDTKSGAGALLLNYEGSDAHDAVRWQTGGAAE
                     LIARRNGQDRVLASGQLVAGPDGWTTLDVSIDGNFLRVRSGGTIVLEGIDDAATGGRF
                     GLQAGAGTRFDKFAAYYREPNEAEEITRQFTREDTMREWAQVAGAWLTPRDAAADFGI
                     WWNKGDFYGDVSLTGEISAAREGDHWAGLILCSDGSSADAGYQLTIRGAGDRGLNLQL
                     NRGGAIATDAVTVEGQAFPAHVSFAKRGSIVSAAVGDRTVLEFRDPQPLSGSRVGVKQ
                     GGLPVSMNSMVAISSDQRDYTFSSSPTDWFVRAGTWDITSRWTCQPEWTWF"
     gene            complement(2469..>3052)
                     /locus_tag="PLQ54_09115"
     CDS             complement(2469..>3052)
                     /locus_tag="PLQ54_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93459.1"
                     /translation="FGRTCQGRPWLRLAVLIGVAALVLAPLVATGAGSSVCFEAEKGT
                     LSGQMKAGFTVSGGGVTAKMQKDIKEQASGRNYIGVPEDGNAEKTPIAGSCTIQFTAP
                     AKAKYTVYLRAWWLDSCANSFKLKFDALPELEIGQDTTYKSWHWVKLNRPIELDGGAH
                     TLVIKNTEDGALADQFYLTAGGPVPQGKMAPTK"
BASE COUNT          470 a         1085 c          987 g          510 t
ORIGIN      
        1 gaaccaggtc cactcgggtt ggcaggtcca gcggctggtg atgtcccacg tgcccgcgcg
       61 cacgaaccag tctgtgggcg acgacgagaa cgtgtagtcc ctctggtcgc ttgagatggc
      121 gaccatcgag ttcatcgaga ccggcaggcc cccttgcttt acgcccacgc gcgaccccga
      181 cagcggttgc ggatcgcgga actcgagcac cgtccggtcg cccacggccg ccgagacgat
      241 actcccgcgc ttggcaaagg agacatgggc cgggaatgcc tgaccctcga ctgtcaccgc
      301 gtccgtggcg atggccccgc cgcgattgag ctgaaggttg agcccccggt cgcccgcacc
      361 ccgaatggtc agctggtatc ccgcatcggc cgacgagccg tcggagcata ggatgagccc
      421 ggcccagtga tccccttccc tggccgcgga gatctcgccg gtcagagaga cgtcgccgta
      481 gaagtcgcct ttgttccacc agatgccgaa gtccgcggcg gcatcgcgcg gggtgagcca
      541 ggcgccggcc acctgggccc actctcgcat ggtgtcctcc cgggtgaact gccgggtgat
      601 ctcctcggcc tcgttgggct cgcgatagta ggcggcgaac ttatcgaacc gagtccccgc
      661 ccccgcctga aggccgaatc ggccgcccgt ggccgcatcg tcgatcccct cgagtacgat
      721 ggtgccaccc gagcgcaccc gcaggaagtt gccgtcaatg gacacatcga gtgttgtcca
      781 cccatcgggg ccggccacga gctggccgga ggccaacaca cgatcctgac cgttccggcg
      841 agcgatgagt tccgctgcgc ctcccgtctg ccagcgcacg gcgtcatgcg cgtcggagcc
      901 ctcgtaattg agcaagagag ccccggcgcc agacttggtg tccacatcgg cacgcaccga
      961 gtagtccgac cacgtctcgt cgccggcaag tgccgtgccc gtttcggcgc tcttccgcac
     1021 aagcacgtgg ccccggctgt cgccaacctt ggccacttgc cagccgtcgg ccggcgcatc
     1081 ccattgggct gtcactccgc tggcatagtc atccacgaag acccggtagc ttctcacgga
     1141 cacgtcgtcg aacttggcgc ccccgggccc gtccgcacac agaccaatct tgccccggct
     1201 cagggccgtt agcccatcga cggccagcac gttcacgccg tccacgtcgg cccggaccgc
     1261 gccatcgtgc agccgaaggc ggagcttgta ccactggttg gtcacgaggc cgccaggagc
     1321 ctcggccagg atcgatggtt gcccgtcacg gaccaggacc agctgatacc ggccggcgtc
     1381 atcctggccc gacgtctgcg acgtcgctcg gaataccagg tagtcgttcg gcccggtgta
     1441 gtaagcgacg agtcccacgg tggccccggt gaggctcttg gcacccgtct gcacctcgta
     1501 gttggtccag tgtgggtagc cggtgacgct cagggccatg ggttcgccgt ggccgacata
     1561 ggcgaacagg ttggtgctcg tctgcgcctg ctgccgccga atcgcctccg ggttcgtcca
     1621 gtcgcccgcc ttggccgtgg tccacgagcc gagggtcctc tcccaggcct ccgccgcctg
     1681 gctgcggttc tcccaggtga agtcctctga gaaggtgatg tcgcccagcg gctgtaccat
     1741 ggcggcacct tcgggaagcc cggggaggtc gtattggacg acgcactggc tgaccgctcg
     1801 cgagtaggcc tcgcacaccg ttgccgggcc cgccaccacc cggatcagtg cgtcatcgcg
     1861 gtggacgacg acggaggcgg gcgcctcggt ggcgggaagc gctccggagg cgagatccct
     1921 gggcggcccg cccttggtct cgccgatcgt cgccctgcca cccgcgaagg cgacctccaa
     1981 agcattcact ccaccggcga gtacgacgcg accctccaga gcggcggggt tgggcacggc
     2041 gatcacgaca tcgaagctcg tggcgccggg atcgcccagt tcggccacgc cgggctgacc
     2101 ccaggccgct ccggcccatg cgagcgctac cacggccatc ggtagtgggc ggaaggcgcg
     2161 ccagcgcctc agcggggagc gcgcactcag tcgcgacatc cgaagtgcct cctcggaacg
     2221 gcggagtcgg cccgaacggg cctacgtatt gccgcgggtg atccatcacg gcacgggtcg
     2281 tcgaacggca ggacggcagg gattgagacg cccaccccga cccgaggttc acgtgcgggt
     2341 caccagcacg gccgcgaagc cccaaccgtg gtcctttgga ccccggccgg ggcttccgag
     2401 ttcctactcc gtctgatgcc ctaccgcaag gacgtgttgg atccctgcgg gttgcggtga
     2461 tctctgcgct acttggtcgg cgccatcttg ccctggggca cgggtccgcc ggccgtgagg
     2521 tagaactggt ctgccagcgc gccgtcctcg gtgttcttga tcaccagggt gtgcgctccg
     2581 ccatcgagct cgatgggccg gttgagcttc acccagtgcc agctcttgta ggtggtgtcc
     2641 tgcccgatct cgagctccgg gagggcatca aacttgagct tgaacgagtt cgcacacgag
     2701 tcgagccacc atgcgcgcag atagaccgtg tacttcgcct tggccggggc cgtgaactgg
     2761 atggtgcacg agcccgcgat gggtgtcttc tccgcattgc catcctcggg cacaccgatg
     2821 tagttcctgc cggaggcctg ttccttgata tccttctgca tcttcgccgt cacgccgccg
     2881 ccactgacgg tgaagcccgc cttcatctgc ccgctgagtg tgcccttctc tgcctcaaag
     2941 cacaccgagc tgcccgcgcc ggtcgcgacc agcggagcga ggaccagggc cgccacaccg
     3001 atgagcaccg ccaggcgcag ccatggccgc ccctggcacg ttcggccgaa cc
//
LOCUS       DAOSPL010000448         3042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_32295_length_3042_cov_2.640777, whole genome shotgun sequence.
ACCESSION   DAOSPL010000448 DAOSPL010000000
VERSION     DAOSPL010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32295_length_3042_cov_2.640777"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..944)
                     /gene="flgG"
                     /locus_tag="PLQ54_09120"
     CDS             complement(159..944)
                     /gene="flgG"
                     /locus_tag="PLQ54_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HOS93460.1"
                     /translation="MMRSLFTAAGGMAAMQLHVDVTANNLSNVNTWGYKRARIDFQDL
                     LSQTLRGPGALSSGGQMIPTGLTVGLGVRPAATVRDFSQGMFVETGLPTDVVIEGQGF
                     YQILLPDGTTAYSRDGSFKLDANGQLVTADGNYLQPQITIPANTIQTTIDSSGTVTVT
                     LPGNVQQNVGQFEHARFVNPAGLLSVGKNLFLETPASGPPITGTPGTDGMGTITQGYL
                     EGSNVQVVSEMVNLIIGQRAFESNSNTIRTSDDMLGTATALRR"
     gene            complement(1223..1375)
                     /locus_tag="PLQ54_09125"
     CDS             complement(1223..1375)
                     /locus_tag="PLQ54_09125"
                     /inference="COORDINATES: protein motif:HMM:NF018168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HOS93461.1"
                     /translation="MKQGYLEGSAVNGIRETVELIQIERAYQAGQRSIEAVDDSLGRL
                     FEKVAG"
     gene            complement(1372..1995)
                     /locus_tag="PLQ54_09130"
     CDS             complement(1372..1995)
                     /locus_tag="PLQ54_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HOS93462.1"
                     /translation="MTRGLYTSASGMLALQVALDATANNLANVATVGYKQDRAVYHDF
                     RDLLISRLYDTPEGPVPPVPWGTPPIGRLGTGVYVEDIATDFTAQGPLQQTGGDLDVA
                     LVGSGFLVVRTPSGERYTRAGQLVRDAQGFLVNDDGHLVLGENGPIRLAADTPTSEIR
                     IAENGEVFERDQRIGRLRIEDLLPGAVKEGYTLVRGTSTGQASGSTV"
     gene            2254..>3042
                     /locus_tag="PLQ54_09135"
     CDS             2254..>3042
                     /locus_tag="PLQ54_09135"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS93463.1"
                     /translation="MEHGPVHGGNGSDARAQQSGPRLVFWELTPKCNLRCVHCRAWAQ
                     EERSADELTTEQGRAFIDSLVAWADPILVLTGGEPLYRPDWRDLAQHASDSGLTVALA
                     TNGTLVDDSTARQIATSGIRRVSISLDGADATTHDGFRGVPGSYAQAVAGLRRLRDLG
                     VSIQINMTVARHNVDQLDEALDRAIALGCDAFHLFLLVPVGCGIRIAETQQIEAERYE
                     DVLAWLAERSKTAQIGLRATCAPHYFRVVRQRGGQMMSPHGHRSR"
BASE COUNT          467 a         1013 c         1027 g          535 t
ORIGIN      
        1 atccgggcca cgtcgcccaa agtcacgggg cccgaggcca cttcgggctg gaggttcagc
       61 gcgatctggg tcttcgccca cgacgcggag gtcatcgcca gggcggcggt gacccacagc
      121 gcagtgcgac acagcgcttt catggtgcat cacccaccct agcgtctcag agccgtggcg
      181 gtgccgagca tgtcatccga cgtgcggatg gtgttggagt tggactcgaa ggcccgctgg
      241 ccgatgatca ggttgaccat ctcgctgaca acctggacgt tggagccctc gaggtagccc
      301 tgggtgatgg tgcccatgcc gtccgtgccc ggtgtccccg tgatgggcgg gccggaggcc
      361 ggcgtctcga ggaagaggtt cttgcccacc gagagcaggc cggccgggtt gacgaatcgc
      421 gcgtgttcga actgcccgac gttctgctgg acattgccgg gcagggtcac ggtcaccgtg
      481 cccgaggaat cgatcgtggt ctggatagta ttggcgggga tcgtgatctg gggctggagg
      541 tagttgccat cggcggtgac cagctggccg ttggcgtcca gcttgaacga tccgtcgcgg
      601 gagtaggcgg tggtaccgtc gggcaggagg atctggtaga agccctggcc ctcgatgacc
      661 acatcggtgg gcagcccggt ctcgacgaac atgccttgcg agaaatcgcg gacggtggcg
      721 gcggggcgga cgccgaggcc cacggtgagc ccggttggga tcatctgccc ccccgacgag
      781 agcgcccccg gccccctcag cgtctggctg agcaggtcct ggaagtcgat tcgagcacgc
      841 ttgtagcccc aggtgttgac gttggatagg ttgttggccg tgacgtcgac gtgcagctgc
      901 atggctgcca tgccgcccgc cgccgtgaac agcgaccgca tcataccgac tcacacctcc
      961 agtggtggtg cagggactct gtcccgagcc ggctgacggt ctggtggcac tgatcggtgg
     1021 gtcgccgaga gtgaggcgag gtgctggaga cccggggaag cccgacgagc cgctccgaag
     1081 gccgcttcgg aactcgccta gccctgccac gggttggggc accgcttctg tcagtccgcg
     1141 gtgcgcggct tccggctccg cgaggagctc ggtccagccg tcgtgcctcg actctcgttg
     1201 gcggcgaatc cgccgctggc gactacccgg ccactttctc gaacagccgg cctagactgt
     1261 catctaccgc ttcgatggat ctctgtcctg cctgatacgc tcgctcgatc tggatcagtt
     1321 cgacggtctc gcggatgccg ttgactgcgg acccttccag gtacccctgc ttcacacagt
     1381 cgagccggac gcctgacccg tcgacgtacc acgaaccaga gtgtacccct ctttgactgc
     1441 ccccggcaac aggtcttcga tccgaaggcg cccgatccgc tggtctcgct cgaagacctc
     1501 accgttctcg gcgatccgga tctccgacgt cggcgtgtcg gccgccagcc ggatgggtcc
     1561 gttctccccc agcaccaggt ggccgtcatc gttcaccagg aacccctgcg cgtctctcac
     1621 gagctggcca gccctcgtgt acctctcgcc cgatggcgtg cgcaccacca ggaagcccga
     1681 accgaccaac gcgacatcca gatcgccccc ggtctgctgc agcgggccct gggcggtgaa
     1741 gtccgtggcg atgtcctcca catagacgcc cgttcccaga cggccgatgg gcggggtccc
     1801 ccacggaact gggggcaccg ggccctccgg cgtatcgtag agcctcgaga tcagcagatc
     1861 ccggaagtcg tgatagaccg ctcgatcctg tttgtacccg accgtggcca cgttggccag
     1921 gttgttggcc gtcgcgtcca gggccacttg cagagcgagc ataccggagg ctgacgtgta
     1981 cagcccccgc gtcaagcgct cacctcctct cccctaagca tgccgtaccc tcgcgatccg
     2041 gggcggagcg cggccgctcc tgagtcccgg tgttgctggg ttttccccgg ggggcccgac
     2101 ttcacctgcc ccgcccgaag attcacgtga ctgtgccccc gccgttgtca ttctgttgcg
     2161 cccggaccgg gagcgttctg catgggggcg gcggttgcgt atgatagggc cggtgatatt
     2221 ggaccacgac gctttccata cgaaggagct cacatggagc atggtcctgt gcacggcggg
     2281 aacgggtcgg acgctcgggc ccagcagtcc gggcctcggc tggtcttctg ggagctgacg
     2341 cccaagtgca acctacgctg cgtccactgt cgcgcatggg cgcaggagga gcgcagcgcc
     2401 gacgagttga cgacggagca gggccgggct ttcatcgata gcctggtcgc atgggccgac
     2461 ccgattctgg ttctcacggg cggggagcct ctgtaccggc cggactggcg cgacctggcc
     2521 cagcacgcat cggacagcgg cctgaccgtc gcgctggcga ccaacgggac gctggtggac
     2581 gactcgacgg cgcggcagat cgcgacgtcg ggcattcgac gagtgagcat cagcctcgac
     2641 ggcgcggatg cgacgaccca tgatggcttc cggggcgtac cgggatcgta cgcccaggcg
     2701 gtggcgggtc tccgccgtct gcgcgatctg ggggtcagca tccagatcaa catgaccgtg
     2761 gcgcggcaca acgtggacca gcttgatgaa gccctcgacc gcgccatcgc cctgggatgc
     2821 gacgcgttcc acctcttcct gttggtgccc gtcggctgtg ggatccgcat cgctgagacg
     2881 cagcagatcg aggctgagcg ctacgaggac gtactcgcgt ggctcgcgga gcgctcgaag
     2941 accgcccaga tcggcctgcg tgcgacctgc gcgccccact acttccgcgt cgtgcgccag
     3001 cgtggcggtc agatgatgtc accccacggc caccgaagcc gt
//
LOCUS       DAOSPL010000449         3038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_32347_length_3038_cov_4.516594, whole genome shotgun sequence.
ACCESSION   DAOSPL010000449 DAOSPL010000000
VERSION     DAOSPL010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32347_length_3038_cov_4.516594"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLQ54_09140"
     CDS             <1..409
                     /locus_tag="PLQ54_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOS93464.1"
                     /translation="QGATEASKTMTALFSSVAAVSLIVGGIGIMNIMLVSVTERTREI
                     GLRKAVGATPGNIQTQFLIEAVTLSLVGGVLGIAAGLVGSFVVKHIGLNAKVSITWIV
                     IAFSFAAAVGVVFGLLPARKAAGLDPVEALRYE"
     gene            402..1778
                     /locus_tag="PLQ54_09145"
     CDS             402..1778
                     /locus_tag="PLQ54_09145"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOS93465.1"
                     /translation="MSESRTHPSTPRCLLGLACLWLCQAVAWSQPGRAEGATAAEASA
                     PKPWTLERCIAVAFRNSPTAWISAEGIASAEAALRQARSGYYPTAELSASATVGSSST
                     SGTGVTSSGNSADADIAVDWTLFQTGRSDLVGESRSELTVARLAHAITLRDLARDVAV
                     DYYAVLASRELVRVAEDGLEAAREHLRDVEARIRHGTAAEVDRFSADADVAQAEVDLI
                     DARTGVRLAVARLRNRIGLDHTAAFDVGPADSEPETPAPSLEEAIAEALQRRPEIAES
                     EAARRAREYAAERAEKARKPSLTVGTSLGYGLSTGTSKGASWRLSGGFSWPLFSRSLE
                     ASEDSARSALRRAQASQRSQALGVGLEVEEALIEVERTAQQIQAGEKAAAAAEARLRA
                     ARGRYANEVGILLDLTDARADLTAAQASLVSVRFDHQVALVSLRHAMGNLPIPSPAET
                     ADGGDRRD"
     gene            1771..2493
                     /locus_tag="PLQ54_09150"
     CDS             1771..2493
                     /locus_tag="PLQ54_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS93466.1"
                     /translation="MIEARGLTKHYRMGDITVEALNDVDLKVEAGEFVAIMGPSGSGK
                     STFMHLIGCLDRPTRGSCRIAGIEVSSLDSDDLAHVRNEKIGFVFQSYNLLATATAIR
                     NVELPLLYARRRLPKEERQARARAALEAVGLGDRMTHRPRELSGGQQQRVAIARALIC
                     QPDIIMGDEPTGNLSSAQGDEIMAILQGLNRKGTTVVIVTHELDIAQHAQRIVHFHDG
                     RVNSDEVVAEPRIARARAHASP"
     gene            complement(2514..>3038)
                     /locus_tag="PLQ54_09155"
     CDS             complement(2514..>3038)
                     /locus_tag="PLQ54_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010046634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS93467.1"
                     /translation="LSDEPVPQSELLEAVRMVLARRGDEATGRDPVTRHTVRQARRQL
                     RVLVAEDHPVNQRLITRLLEKAGHVVTVASDGREAIGAYAPGRFDLVLMDVQMPEMDG
                     LEATAVIREAEQASGIGQRVPVIALTAHALGADRERFLAAGMDEHVPKPVTADALFDT
                     IERLVRPNEPGFRA"
BASE COUNT          477 a         1048 c         1030 g          483 t
ORIGIN      
        1 ccagggggcc accgaggcca gcaagaccat gactgccctg ttcagctccg tggccgccgt
       61 gtctctgatc gtgggcggta ttggcatcat gaacatcatg ctggtctcgg tcaccgagcg
      121 gacccgcgag atcggcctcc gcaaagccgt gggggccact cccggcaaca tccagacgca
      181 gtttctgatt gaggccgtca cgttgagcct cgtgggcggg gttctcggaa tcgcggcagg
      241 tcttgtcggc tcgttcgtcg tgaagcacat cggactcaat gcgaaggtgt ccatcacgtg
      301 gatcgtcatc gcgttctctt tcgccgcggc ggtgggtgtc gtgttcggac tcctgcccgc
      361 caggaaagcg gcgggcctag acccagtgga ggccctgagg tatgagtgag tcgagaaccc
      421 acccgagtac accgaggtgc ctgctcggcc tcgcgtgcct gtggctctgc caggcagtag
      481 cctggtccca gccgggccgt gccgagggtg ccactgctgc cgaggcgtcg gccccgaagc
      541 cctggacgct ggagcgatgc atcgccgtgg cctttcggaa cagcccgacg gcatggatct
      601 ccgccgaagg catcgccagc gccgaagccg cgcttcggca ggcgcgtagc ggctactatc
      661 ccaccgccga gctgtcggcc tcggctacgg tgggtagctc ctcgacatcg ggcaccggag
      721 tgacgtcctc ggggaactcg gccgacgccg acatcgccgt cgactggacg ctcttccaga
      781 ctggccgtag cgatctggtt ggcgagagtc gttcggagct caccgtggcg cgtcttgccc
      841 acgccatcac cctccgggat ctggcgcgtg atgtcgccgt tgactactac gccgttctgg
      901 cgtctcgcga gcttgtgcga gtggccgagg atggcctgga agcggcccgc gagcacctcc
      961 gcgatgtgga ggcgcgtatc cgccacggca cggccgctga agtagaccgg ttctcagccg
     1021 acgcggatgt ggcccaggcc gaggttgacc tgatcgatgc ccggacgggt gtccgactgg
     1081 cggtcgctcg gctgcggaac cggatcggcc tcgatcacac ggccgccttc gacgtcggtc
     1141 ccgcggattc cgagccggag acgcccgccc cctcgctcga ggaggccatc gccgaggcgc
     1201 tgcagcgccg gcccgagatc gccgagagcg aggccgcccg gcgtgcccgc gagtacgccg
     1261 ctgaacgggc cgaaaaggcc cggaaaccct cgctgaccgt gggcacctcc ttgggctacg
     1321 gtctgagtac cggcaccagc aagggggcgt cgtggcgtct gtcggggggc ttctcgtggc
     1381 cgctgttctc acgctcgctg gaggcctccg aggacagcgc gaggtccgcc ctgcggcgtg
     1441 cccaggccag ccagcgttcg caagcgctgg gcgtgggcct cgaggtcgag gaggcactca
     1501 ttgaggtcga gcgcaccgct cagcagatcc aagccggcga gaaggccgcc gccgcggccg
     1561 aggcccggct ccgagcggcg agaggacgct atgccaacga ggtgggcatc ctgctggatc
     1621 tcactgacgc ccgggccgac ctgaccgccg cgcaggcgag cctggtcagt gtccggttcg
     1681 accaccaggt cgcgctggtc tcgctccgcc atgccatggg caacctgccc attccctcgc
     1741 ccgcagagac ggccgacggg ggcgaccgcc gtgattgagg cccgcggact gaccaagcac
     1801 taccggatgg gcgacatcac ggttgaggca ctcaacgacg tggacctcaa ggtcgaagcg
     1861 ggcgagttcg tagccatcat ggggccatct gggtccggca agtcgacctt catgcacctg
     1921 atcggatgtc tcgatcgccc cacgcgggga agctgccgca tcgctgggat cgaggtctcc
     1981 tcgctggata gtgatgacct cgcccacgtg cgcaacgaga agatcggctt cgtcttccag
     2041 agctacaatc tgctcgccac ggccaccgcc atccggaacg tggagctgcc cctgctctac
     2101 gcgcgccgcc ggctgcccaa ggaggaacgg caggcccgtg cgcgcgccgc gctggaggcg
     2161 gtggggctgg gtgatcggat gacccacagg ccccgggagc tctcgggcgg ccaacagcag
     2221 cgcgtggcca ttgctcgtgc gctcatctgt cagccggaca tcatcatggg tgacgagccg
     2281 accggaaatc tctccagcgc acaaggcgac gagatcatgg ccatcctgca gggcctgaac
     2341 cggaagggca ccactgtggt catcgtcacc cacgagctgg acatcgccca gcacgcccaa
     2401 cgcatcgtcc acttccacga cggccgcgtc aacagcgacg aggtggtggc cgagccgcgc
     2461 atcgcccgcg cccgagccca tgcgagccca tgacggccac ccatcacagt cggctaggcc
     2521 cgaaagcccg gctcattggg ccggaccagc cgctcgatgg tgtcgaacag cgcgtcggcc
     2581 gtgacgggtt tgggcacgtg ctcatccatt cccgccgcga ggaaccgctc gcgatcggcg
     2641 ccgagcgcgt gagccgtgag cgcgatcacg ggcacccgct ggccgatgcc gctggcttgc
     2701 tcggcctctc ggatcaccgc ggtggcctcc aggccatcca tctctggcat ctggacatcc
     2761 atcaggacca agtcgaagcg tccgggcgca tatgccccga tggcttcgcg tccgtcgctc
     2821 gctacggtaa cgacgtgccc ggccttctcg aggagccggg tgatcagtct ctggttcaca
     2881 gggtgatcct cggccaccag gacccggagt tggcggcgtg cttgacgcac cgtgtgtcgg
     2941 gtgaccgggt cgcgcccggt cgcctcatcc ccgcggcgag ccagaaccat gcgcaccgct
     3001 tccagcagct ccgactgcgg gacgggctcg tcagatag
//
LOCUS       DAOSPL010000450         3036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_32384_length_3036_cov_2.840657, whole genome shotgun sequence.
ACCESSION   DAOSPL010000450 DAOSPL010000000
VERSION     DAOSPL010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32384_length_3036_cov_2.840657"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..135)
                     /locus_tag="PLQ54_09160"
     CDS             complement(<1..135)
                     /locus_tag="PLQ54_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
                     /protein_id="HOS93468.1"
                     /translation="MVSVLLESTTKAFGNTIAVDDVTLNIGAGELFFLLGPSGCGKTT
                     C"
     gene            337..939
                     /locus_tag="PLQ54_09165"
     CDS             337..939
                     /locus_tag="PLQ54_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93469.1"
                     /translation="MARADSRLVMICAIIGLVGLACGVAAAISTGSMTTQQVGVAQVG
                     QSPPPAPVAPAAKPDLKSRLTQGGMSEAEADALQKQLQGLGVTDDMIAKFLSGDLKAA
                     EAAAKSAGIEANAVLAAVSPYLAKYPGIVAALQGKGGPTASAPPEGAAISRLKWRFPD
                     REFTQISPKYIVDDQGNRYVVGATNFCFPVDAHNIIGRAI"
     gene            961..1539
                     /locus_tag="PLQ54_09170"
     CDS             961..1539
                     /locus_tag="PLQ54_09170"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOS93470.1"
                     /translation="MDASAAKSELRARAIAFRRGLSPGEVRDRGWRMWALLGNVPRYA
                     LAQGILVCASFDNEPDTCAVLERAPRVKRVYVPRAIVASRELAIHPYPTELHPSRWGV
                     PEPLPTAPVVAPQEVRRVVDVAVLVGLAFGRGNLVRLGYGAGFFDRFLSAHPVYAIGL
                     CYDELLVDGLPRSEWDVPMHAVVTDRRVLFRV"
     gene            complement(2053..>3036)
                     /locus_tag="PLQ54_09175"
     CDS             complement(2053..>3036)
                     /locus_tag="PLQ54_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93471.1"
                     /translation="RPTHTGRVRLRYGSPFGVVSGREGFYVDTEFEFHTNGGGDGKLV
                     VDGLLTPTYGATLRYTHEFDDGKRGSAYISWPQHSYLTANANLWRSGRSVDNIINARV
                     MGGGSLSTTYSLDSSWNFKPKSTIGGWRWQYSLDTQFARDSYDTRPYLEGGVRLRLHP
                     GKLYQFGNKVSLSPRISGGAWLDTLGRTETLASGSADLNFNITNGTVLGLSYSAEHRH
                     GGRFTTGFSHSVTARLNAFSGERWWTSASATYNLTNGSSFGVAYGSWEFIPKWHLDAS
                     ALFQRYGLASFSDYMVALGRDIGGQQARIVYSTSEGRLYFELRRAGGFFTF"
BASE COUNT          481 a          962 c         1071 g          522 t
ORIGIN      
        1 gcaagtcgtc ttgccgcacc cggatgggcc gagcaggaag aacagctcgc ccgcgccgat
       61 attgagtgtc acgtcatcga cggcgatggt gttgccgaat gctttggtgg tggactcgag
      121 caagacggag accacggcca cacccctgag gcaatgttcg gctccatggg cgcccccacc
      181 tgtcgcgcgt ccggggctct gccaggagca ggagcatgcg cgtgcgcgga gaaggtatac
      241 aagcctggcc gtgaacagcg tgccagacct agcgttttgc tcggtgagcc cgaatggggt
      301 ctgagagcgc ccgatgggca ggagggctgc acgtctatgg cacgcgcgga cagccgtttg
      361 gtcatgatat gcgccatcat tggtctggtg ggtctggctt gcggggtggc ggcggccatc
      421 tcaacgggga gcatgaccac ccagcaggtg ggtgtggctc aggtcggtca gagtccgccg
      481 cccgcgccgg tggcccctgc cgccaaacca gacctgaagt cgcggctgac gcagggcggc
      541 atgtcggaag ccgaggcgga cgccctgcag aagcagcttc aggggctggg ggtcaccgat
      601 gatatgatcg cgaagttcct atcgggcgac ctcaaggcgg ctgaagcggc cgcgaagtcg
      661 gccggcatcg aggcgaacgc cgttctggcc gcggtgagcc cctatctggc gaagtatcct
      721 ggcatcgtgg cggcgctcca gggcaagggc ggacccacgg cctcggcgcc tcccgagggt
      781 gcggccattt cgcggctgaa gtggcggttc ccggaccgcg agttcacgca gatctcgccc
      841 aagtacatcg tcgatgatca gggcaaccgg tatgtggtgg gcgccaccaa cttctgtttc
      901 ccggtggatg cgcacaacat cattgggcgg gccatctagc ccgccggcca ttccagcgta
      961 gtggacgcct ctgcagccaa gtccgagctg cgcgcgcgcg ccattgcctt tcgccggggc
     1021 ctctcacccg gcgaggttcg ggaccgcggc tggcgtatgt gggccctgct cggcaacgtg
     1081 ccgcggtacg ccctcgctca gggcattctg gtctgtgcct ccttcgacaa cgagccggac
     1141 acgtgtgcgg tgttggagcg ggcgccgcgc gtgaagcgcg tgtatgtccc ccgagctatc
     1201 gttgcctcac gcgaactcgc gatccatccc taccccaccg agctgcaccc aagcagatgg
     1261 ggagtgcctg agcccctgcc tacggccccg gtagtcgcgc cgcaggaggt ccggagggtg
     1321 gtggacgtgg cggtgctggt cggcttggca ttcgggcgcg gcaacctcgt tcgactgggc
     1381 tacggggcgg gcttcttcga ccgatttctc agtgcccatc cggtctacgc catcggcctg
     1441 tgctacgacg agctgctggt ggacggactg ccacgctcag agtgggatgt gccgatgcac
     1501 gcagtggtga cagaccgacg ggtcctgttc cgcgtctgag gaggcggggc tgtgggcgga
     1561 gcgccggtga gcgtgtgacg cccctgcgga cgccgcttgg ggcgtgggga gaggtgctgc
     1621 ccgcgctgcg gtcgcgaacg gggacccgag tgcgtgcctc caggccgcgc ggcgggtgtc
     1681 tggcggatcg ggcgcagtcc cgacgcctac gcggcggaca acgatggact gctccccgag
     1741 tacggcacgg acctcgcggc cgccatggcg ccctatctgc cgtggcgcga ctaccgctgc
     1801 cggctggtgc caccccgcag cgcgccctcg ttcaccgtgg cccccgggcc cagccgcgcg
     1861 ctcgcctctg attcccgcgg ggacccagag gccccttact catcgagacg gcattccggc
     1921 acgagttcag gggagaccag atgatggcgg tggtgttcgc cgacggccgt gggagaacgg
     1981 caagcccaga tgccctgtgg cttccgccac agccgccatg gtccgagccc gcgtccgacc
     2041 agcggatggg cctcagaagg tgaagaaacc gcccgctcga cggagctcga agtagagccg
     2101 gccctcggag gtcgagtaga cgatgcgggc ttgctgtccg ccgatgtcgc ggcccagggc
     2161 gaccatgtag tcgctgaagg atgccaaccc atacctctgg aaaagggcgc tggcgtcgag
     2221 gtgccacttg ggtatgaact cccacgagcc ataggccacg ccgaatgagg agccattcgt
     2281 caggttgtag gtggccgatg ccgacgtcca ccagcgctcg ccggagaagg cgttcagacg
     2341 ggccgtcaca ctgtggctga aaccggtggt gaatcggcct ccatgccggt gctcggccga
     2401 gtagctcagt ccaaggaccg ttccgttggt gatgttgaag ttgaggtcgg ccgagccact
     2461 tgccagcgtt tcggtccggc cgagagtgtc cagccaagcg ccgccgctga tgcggggcga
     2521 caaggagacc ttgttgccga actggtagag cttccccggg tggagccgaa ggcgaacccc
     2581 gccctcaagg tatggccgcg tgtcgtacga gtctcgggcg aactgcgtgt ccaggctgta
     2641 ctgccaccgc cagccaccga tggtgctctt gggtttgaag ttccaagagg agtcgagcga
     2701 gtacgtcgtg gagaggctgc cgccgcccat gacccttgca ttgatgatgt tgtctacact
     2761 tcggccggac cgccagaggt ttgcgttggc agtcaggtag ctgtgctgcg gccacgagat
     2821 gtacgcggat cctcgcttgc catcatcgaa ctcgtgcgtg tagcggagtg tggcgccata
     2881 ggttggggtc agcagcccgt ccacgacgag cttgccgtcc ccgcctccgt tggtgtggaa
     2941 ctcgaactcg gtgtcgacgt agaacccctc gcggcccgag acgacaccga agggactgcc
     3001 gtatcgcagc cgcactcgtc ccgtgtgggt cggtcg
//
LOCUS       DAOSPL010000451         3033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_32424_length_3033_cov_2.742780, whole genome shotgun sequence.
ACCESSION   DAOSPL010000451 DAOSPL010000000
VERSION     DAOSPL010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32424_length_3033_cov_2.742780"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PLQ54_09180"
     CDS             complement(<1..520)
                     /locus_tag="PLQ54_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOS93472.1"
                     /translation="MSWCRDISAEVEVESSVREASGQISGASGDVARGVSETTAAAEQ
                     IATGVQGISSEMESVSAAASRGVEVAARGSEAALSTVASIGSVRESITTIVEGMRALN
                     AQAEQIGSIIDVIDGVADQTNLLALNAAIEAARAGEHGKGFAVVADEVRKLAERATQA
                     TDEVNQLLQGIRQ"
     gene            complement(480..1349)
                     /locus_tag="PLQ54_09185"
     CDS             complement(480..1349)
                     /locus_tag="PLQ54_09185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOS93473.1"
                     /translation="MTRSDTRAPWLSHLQATLADPIVDTRCGWKDRRRRIEDLATTLE
                     GAARVLHDLPAASFVADERLSLQLVNPALAELAGRDAEQIAGSLSVSDLLTAAEGEWH
                     SMVVPCLGHGQVLRGIEAELAAPGQKAGRVSVDLGPLRDARGVVCGIVGTVRDVSAER
                     EREREAADKAAWVKGILERIPDPFFQVDSNLTVTYMNPACAAAVGYDASEVVGKMRCA
                     DVFRSNICETNCAIKHCMRTGESITGARVTIQNRRGDRIPILCSAAALTDSQGQIIGG
                     YELVSGHLSRGRG"
     gene            2493..>3033
                     /locus_tag="PLQ54_09190"
     CDS             2493..>3033
                     /locus_tag="PLQ54_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93474.1"
                     /translation="MTMRCVGLLLGAVACAGLVSGCSPAVTVLGPASISETAGMVTLP
                     EVDQPDYVVREDLWRRSHTGGVALRVDLPSMPAAGLDCVAMTGRSTLSSSGEGRVDAV
                     DPSAAIMAILTPRGSREPAAVVYVPAAAAGQTRALGAREVALGCVATHPSLLLSPAAA
                     RDAALRWVQGQPGFEALVAD"
BASE COUNT          477 a         1063 c         1010 g          483 t
ORIGIN      
        1 gttgccggat gccctggagg agctggttga cctcatcagt ggcctgagtc gcccgctcgg
       61 cgagtttgcg cacctcgtcc gccacgacgg cgaaaccctt cccatgctcg ccggcccggg
      121 cggcctcgat ggcggcgttg agggcgagca ggttggtctg gtccgccacg ccgtcgatga
      181 catcaatgat gctcccgatc tgctccgcct gggcgttgag agccctcata ccttcaacga
      241 tggtggtaat agactcacga acacttccaa tcgacgccac cgtcgacagc gccgcctcgc
      301 ttcctcgcgc ggcaacctcg acgccgcgtg acgccgccgc ggacacgctc tccatctcac
      361 tcgagatccc ctgaacgccc gtggcgatct gctcggcggc ggcggtggtc tcgctgacgc
      421 cccgagcgac atccccactg gcccccgaga tctgcccaga ggcctcgcgg accgacgact
      481 caacctcgac ctcggctgag atgtcccgac accagctcat accccccgat gatctggccc
      541 tggctgtccg tcagagcggc ggccgagcac agaatgggga tccggtcgcc gcgccggttc
      601 tggatggtga cccgggcacc ggtgatgctc tcacccgttc tcatgcagtg tttgatggcg
      661 cagttggtct cacagatgtt gcttcggaac acgtcggcgc acctcatctt gccgacgacc
      721 tcggaggcgt cgtacccgac cgcggcggca caagccgggt tcatgtacgt gactgtgagg
      781 ttcgaatcga cctgaaagaa cgggtcgggg atgcgttcca gaatgccctt cacccatgcg
      841 gccttgtcgg ctgcttcccg ctcgcgttca cgctcggccg acacatcacg cacggttccg
      901 acgatgccac agacgacccc acgagcatcg cgcagcgggc ccaggtcgac cgaaacgcga
      961 ccagccttct gaccgggggc cgcgagctcg gcctcgattc cacgcagcac ttggccgtgg
     1021 ccgaggcagg gaaccaccat agagtgccac tcgccctccg ccgccgtgag cagatcgctt
     1081 acggagaggc tgcccgcgat ctgctcggcg tcgcggccgg cgagctccgc cagagcgggg
     1141 ttcacgagct gcagactcag gcgctcgtca gccacgaacg acgcggccgg aaggtcgtgc
     1201 aggactcgcg cggccccctc gagtgtggtg gcaagatcct caattctcct acggcggtcc
     1261 ttccatccgc accgagtgtc gacaatgggg tcggctagcg tggcctgcag gtgcgacagc
     1321 cagggcgctc tcgtgtcact acgggtcata cttcgtaccc cccctaagcc tcgggcctgt
     1381 cccgctcaca gggcaaggca ccgatgttct gggggtagtt atgccggggg cgaattgccg
     1441 attggagggg gatcgccatt atccgcttgc tgcaagtaat gtggtggatg cacggggccg
     1501 gcattcgaac gctcgaccgc gaggacctag ctcggcctgg cctccctcgg gcagatagat
     1561 gatacactca acgccggtgc ccgccgtcgc ggccgagcgg gatccaggcg gcgcaggtgt
     1621 ccagcgactt tcccgtctcg cgaccgaacc cagcgaatgg gtctgcccag cccatacaag
     1681 cccgaccaga ctggcgaaag acccgcagaa gacggctgtg acgctggaag cccgtcgcgc
     1741 ggaagacccg tacaccgcac tgccctgtct cgtggagggg cgcccgccgg ccccaactgg
     1801 aggcccgcgg gctactgcac gcctcgggcc gatccggtgc cccgcaccct tcgggtgcac
     1861 ccctgtgcgg accccccgaa catcctccag gcaaccgagg agggaccgaa tggaaggggc
     1921 ccgcgaagcg ggaagggcgc catccggccc atgggctgcc ggagtcccag gttaccgcca
     1981 acgggagcac ggggacccgc agtcgcgaac ccgctcggtg gccggaggag ctgcgggagc
     2041 tcagcgaggc actcctgcag gaccccacca ccctcggata ccgcgctgac ctctgggccc
     2101 tggctcccct cgccccgctc accaagaagc acgtcgctgt gcgacggtcc gtgacccggg
     2161 tgtggcgaga gctgcaggac atggacctga ggcccgcgcg acgagccgtg tgagccgatg
     2221 agcagcggat cgcggcgata gctccagagc ggcgggctcc cctcggtcca acacagcttc
     2281 ttcgcctcgc tgccgagatc tcgggtgccc aactcaggat ggcaccccag ggccaacgcc
     2341 gagcccgggt cttatccacg gagcgctctg cagccgcgcg taggccgagc ggctgagcgt
     2401 tgtgagtacc cctctggatg ggttaccatc aggagggccg caacacccct gcgctcgagc
     2461 atgctgaagc ccgcgaaagg ggaacggttc ccatgaccat gaggtgcgtc ggcttgctgc
     2521 tcggcgcggt ggcgtgcgcc ggcctggtgt cgggctgctc cccggcggtc acagtgctgg
     2581 gcccggcctc catctcggag accgctggga tggtgacgct cccggaggtc gaccagcccg
     2641 actacgtggt gcgcgaggac ctgtggcgcc ggtcccacac aggcggtgtc gcactgcggg
     2701 tggatcttcc ctccatgccc gcggccggtc tggactgtgt ggccatgaca ggccgtagta
     2761 ccctcagttc gtcgggcgag ggacgcgtcg atgcggtcga tccgtccgcg gcgatcatgg
     2821 ccatcctgac gcctcgaggc tcgcgggagc cggcggccgt ggtctacgtg ccggcggcgg
     2881 ctgcgggcca gacccgggcc ctgggcgcgc gtgaggtcgc attggggtgc gtggcgaccc
     2941 acccatcgct actcctatcg ccagcggcgg ctcgtgacgc tgcattgcgg tgggtccagg
     3001 ggcagccggg attcgaagcc ctggtcgccg acg
//
LOCUS       DAOSPL010000452         3029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_32467_length_3029_cov_2.980834, whole genome shotgun sequence.
ACCESSION   DAOSPL010000452 DAOSPL010000000
VERSION     DAOSPL010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32467_length_3029_cov_2.980834"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1846)
                     /locus_tag="PLQ54_09195"
     CDS             complement(<1..1846)
                     /locus_tag="PLQ54_09195"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS93475.1"
                     /translation="MGSCWPSRWLVVVLLGCATPLWPAAEEVPGAFTDELIEAVVWRA
                     SESEVRLVTDPAPGIDRALEWVVTTQAGRVNTLERELPVRPDAAGVIHFRIHCGTRAA
                     VEVQLAGSGDARLYHIVQLDGSDWHDVSVPIARMARFGGFTDDMLAGPLALRFWIDGA
                     PGWAGGAHQPMRQLTFTIADIRIDAPLAGRPEGVGALIEVDPSHLEGRIDPNVYGHFL
                     EHIYHSVGDGLCGELIRNRSFVPPAGGFELADGVLTQTSPRTDEKLLAGDPQWTDYEF
                     SLRARKTGGQEGFLILVRAASDDDFYWWNLGGWGNTQHALECEVAGARRVITEPVRGA
                     IEPDRWYAVRVRVEGDRIEGWLDGEKLLDARDVSHPRGGVGLGTWATQAEFAGMRVRD
                     SAGRELPLAFEPPVQEQRVPEFWEPVPVGAPVSQTRTDSPNTGWSVRVELAGPREWRG
                     IRQGRIHAVAEHRFVGRAWLRHEGLLEGAKAVLRGASGSVISECRFSPPPGEGGWTEC
                     RFELAATVDDPEAWLEILGKGYGTLWVDQVSLMRDDSIGTGCRRDLLEAVRNLQPPII
                     RWPGGCFASIYRWKSSVGPQNERKPFYNAPWGEWDDASFGTDEFIRLCR"
     gene            1944..3002
                     /locus_tag="PLQ54_09200"
     CDS             1944..3002
                     /locus_tag="PLQ54_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93476.1"
                     /translation="MYQMRALCGAGLAALLCAAPRGSPAPPAVDRNACPNACFSEGFA
                     HWSLAASGGSITRVVADGPGGGWSAEIDGLGAAGEAGIVSDPFPVRPSVVYRLAAQVK
                     RVSGDGPYKVSLEWLDADGGHLAYANSWTGVLAGSQWEYHHVELISPGTAASARILAG
                     IRAGCHAQMTAFELCELGPAEARLAIDLVPEPVAATSPWPLRVRLENRGGKELDRIAV
                     ELRLPAGVTARERPGLSVAALPPGQVWAADVLLAGDPGDGEIRCCVTAVADGKRRTWS
                     AATHPFVSTSRTEERATHDLSPPYRPRTCLKLGGFYFPVMLDWDRSGWGVRRVPRMQP
                     LLGYYDESLPQVADWHIT"
BASE COUNT          462 a         1074 c         1047 g          446 t
ORIGIN      
        1 cgcggcagag ccggatgaac tcgtcggtgc cgaagctcgc atcgtcccac tcgccccagg
       61 gagcgttgta gaacggcttt cgctcgttct ggggaccgac gctgctcttc cagcggtaga
      121 tggaagcgaa gcatccaccg ggccaccgga tgatgggcgg ttggaggtta cggacggcct
      181 cgagcagatc ccgtcggcag ccggtgccga tcgagtcgtc gcgcatcaac gagacctgat
      241 cgacccacag cgtaccgtat cccttgccga ggatctcgag ccatgcctcc gggtcgtcca
      301 cggtggcggc cagttcgaac cggcactcgg tccagccccc ctcgcccggc ggcggagaga
      361 acctgcactc gctgatgacg gatcccgaag cgccccgaag gaccgccttg gcgccttcga
      421 gcaggccctc gtgccggagc catgctcggc cgacaaagcg gtgctcggcc accgcgtgga
      481 tccgcccctg acggatcccg cgccactctc ggggcccggc gagctccacg cgcaccgacc
      541 atccggtgtt gggggagtcg gtccgggtct gcgacacggg cgcgccgacg ggaacgggct
      601 cccagaactc gggcacgcgc tgctcctgga cgggcggctc gaacgccagc ggcagctcgc
      661 ggccggccga gtcgcggaca cgcatgccgg cgaactcggc ctgggtcgcc caggttccga
      721 ggcccacgcc gccccgaggg tgcgacacat cgcgggcgtc gagcaacttc tcaccgtcga
      781 gccagccctc gatgcggtcc ccttcgaccc gcacgcgaac cgcgtaccac cggtcgggct
      841 cgatggcgcc ccgtaccggc tcggtgatga ctctgcgggc gcccgcaacc tcgcactcga
      901 gtgcgtgctg ggtgttgccc catccgccga ggttccacca gtagaagtcg tcgtcggaag
      961 cggcgcgcac caggatgagg aagccttcct gcccgcccgt cttccgggcg cgcaacgaga
     1021 actcgtagtc ggtccactgg gggtcgccgg ccagcagctt ctcatcggtc cggggcgagg
     1081 tctgggtgag gacgccatcg gcgagctcga acccgccggc cggcggcaca aagctccggt
     1141 tgcggatcag ctccccacac agcccgtcac cgaccgagtg gtagatatgc tcgaggaagt
     1201 ggccgtacac gtttgggtcg atacggccct cgagatgaga gggatccacc tcaatgagcg
     1261 cacccacgcc ctcgggccgc ccggccaggg gcgcatcgat gcggatatcg gcgattgtga
     1321 aggtgagttg ccgcatgggc tggtgagcgc cgccggccca gccgggcgcg ccgtcgatcc
     1381 agaaacgaag cgccagcggc cccgcgagca tgtcgtcggt gaagccgccg aaccgggcca
     1441 tacgggcaat cggcaccgag acgtcgtgcc agtcggaccc atcgagctgc acgatgtggt
     1501 acagccgcgc gtcgccagag ccggcgagct ggacctcgac cgcggcgcgc gtgccgcaat
     1561 ggatgcggaa atggatgacc ccggcagcgt cgggccgcac cggcagctcg cgttccagag
     1621 tgttcacccg gcccgcctgc gtggtgacga cccactcgag cgcccgatcg atcccgggtg
     1681 ccggatccgt cacgagtctg acctctgact cggacgcacg ccagaccacg gcctcgatca
     1741 gctcatcggt gaaggcgccg ggtacctcct cggccgcggg ccacagcggc gtcgcgcagc
     1801 ccaggaggac aacgaccaac catcgggacg gccagcaact gcccatacca atcacccctg
     1861 cggcaacgca gagcgagggt tccccggagc actccgggaa tcctgggtga ggcgacacgc
     1921 cctcgccgga gatggagccg cgaatgtacc agatgagagc gctgtgtggg gcgggcctgg
     1981 ccgcgctgct gtgtgcggcc ccgagaggtt cgccggcgcc gccggcggta gatcgcaacg
     2041 cctgcccgaa cgcgtgcttc tccgagggct tcgctcactg gagcctcgcg gcatcgggcg
     2101 gctcgatcac gcgggtcgtc gccgatgggc cgggaggcgg ctggtcggcc gagatcgatg
     2161 gcctcggggc ggccggcgag gctggcatcg tatccgaccc attccccgtg cggccgtcgg
     2221 ttgtctaccg gctggcggca caggtgaaac gcgtctcggg tgacggcccg tacaaggtct
     2281 cgctcgagtg gctcgacgcc gatggcggcc acctcgccta cgcgaactcg tggaccggcg
     2341 tactggccgg cagccagtgg gagtaccacc acgtcgagct catctcaccg ggcaccgcgg
     2401 catcggcgcg cattctcgct gggatccgtg ccggctgcca tgcgcagatg acggctttcg
     2461 agctctgcga gcttgggccc gccgaggccc ggctcgcgat cgacctggtg ccggagcctg
     2521 tggcggcgac gagtccgtgg ccgttgcggg tccgcctcga gaaccggggt ggtaaggaac
     2581 tcgatcggat cgccgtcgag ttacgcctcc ctgcaggtgt gacggcccga gaacgccccg
     2641 gcctgtccgt cgccgctctg cctcccggcc aggtctgggc cgcagacgtg ctcctggccg
     2701 gcgatccggg agacggcgag atccgctgct gtgtgaccgc ggtggccgac ggcaagcggc
     2761 ggacctggtc ggcggcgacc caccccttcg tctcgacctc gcgaaccgag gagcgggcga
     2821 cgcatgacct ctctccgcct taccggccgc gcacctgcct gaagctgggc ggcttctact
     2881 tccccgtcat gctcgactgg gaccgcagtg gctggggcgt ccgaagggtt cctcgcatgc
     2941 agccgctact cggctactat gacgagtcgc tcccccaggt ggccgactgg cacatcacgt
     3001 gagccacgga gcacgggctg tccttcttc
//
LOCUS       DAOSPL010000453         3026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_32510_length_3026_cov_2.937058, whole genome shotgun sequence.
ACCESSION   DAOSPL010000453 DAOSPL010000000
VERSION     DAOSPL010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32510_length_3026_cov_2.937058"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLQ54_09205"
     CDS             <1..250
                     /locus_tag="PLQ54_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019930178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOS93477.1"
                     /translation="QIWSDNPQERLNREVRRRTDVVGIFPNREAVIRLVGMVLAEQHD
                     EWAVGRRSMSTGSLAKLRPARVIELEEPEEVTAQLLAS"
     gene            583..2259
                     /locus_tag="PLQ54_09210"
     CDS             583..2259
                     /locus_tag="PLQ54_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOS93478.1"
                     /translation="MRVGTTQDADNLNPFIGYSVTAYQVYHLNYDLLVGYAPNGDSRP
                     ELATSWTVSTDGLQWTFKLREDVKWHDGVPFTARDVAFTYNYIIDNDLAAFSTFTKGI
                     DKAVAVDDMTVKFYLRKPKANMLRLWIPIVPEHIWSKISGEEASAAFQNRPPIVGTGP
                     FKTVEAKKGSFIRMEANKDYWGGAPDIDELIIQVYTNQDTMTMDLKGKVIDAAVGLPV
                     GQFESLKKVDGIAAVAARQRYFTELAMNCYTGPSLGNPVLKDVNFRRAISWCVDKEKL
                     IDTCWGGYADRGDSILAPGTDYAWQPSESERFGYDLDKATELLAEAGYRDTDGDQILN
                     DLHNANRDIKLRLWTREESPEQQRAGKLIAGALESVGIDIELSVMNDGAISDGIYNYE
                     DGTYKPNFDMFIWGWQGSADPDYQLADFVTDQIEMWNDCCWSNERYDKLRAQESTETD
                     PARREQEVVEMQKIFYESAPYVVIAYPRKLVAYDCDQWEGWVPYPGKEGDPVFSYDNV
                     DTYVKLAVKTGSESGGGSGGIVAVVIVVVIVAAIVVFILVRRRTREAEEE"
     gene            2479..2664
                     /locus_tag="PLQ54_09215"
     CDS             2479..2664
                     /locus_tag="PLQ54_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93479.1"
                     /translation="MAFRLGNHVIVDNPFGWMKTVGRLAKLRHRGKAKAVDLHTFACA
                     AFNLVRLRTLLAEPSSA"
BASE COUNT          589 a          959 c          965 g          513 t
ORIGIN      
        1 ccagatctgg agcgacaacc cccaggagcg cttgaaccgc gaggtccgcc gcaggaccga
       61 cgtggtcggc atcttcccga accgtgaggc agtgatccgc ctcgtcggca tggtgctcgc
      121 cgaacagcat gacgagtggg cggtgggacg ccgctccatg agcaccggtt cgctcgccaa
      181 gctgcggcca gcgagggtca tcgagctcga ggagcccgag gaggtgacgg cccagctcct
      241 ggcgagctga ggacgagaga agcggatgga gcggagactc tcgtacacca ccttgacgga
      301 cgtggccctt tcgagcactt tgttttcggc gcaatgcgca gccttgacca cccggcgacg
      361 gccgtggtat tttcgcggcc actcacccgg cccatgtttg gtgacacttc ggctggtttc
      421 gaccatcggg ggaacgtgat gtccgaacgt acttggtgcc tcacatggag aggcatcgca
      481 gttgtgacgt tgacggcgct cgtcgtcttg agtttgacga aggcaacgtc acctgccgcg
      541 gcgtcgacat cgtcatcgcc accgactagc gctgaggtcg tcatgcgggt aggtaccacc
      601 caagatgcgg acaacctcaa cccgttcatc ggctactcgg tcactgccta ccaagtctat
      661 cacttgaact acgaccttct ggttggctac gctccgaacg gcgattcccg ccccgagctc
      721 gccacgagct ggaccgtgag caccgacggt ctgcagtgga cgttcaagct ccgcgaggac
      781 gtgaagtggc acgacggcgt tcccttcacc gctcgcgatg tcgccttcac ctacaactac
      841 atcatcgaca acgacctggc cgccttctcc accttcacga aaggcatcga caaggcggtt
      901 gccgtggacg acatgacggt caagttctac ttgcggaagc ccaaggccaa catgctgcgc
      961 ctctggatcc cgatagtgcc cgagcacatc tggagcaaga tctccggtga agaggcgagc
     1021 gcggcctttc agaacagacc gcccatcgtc ggtactggtc ccttcaagac cgtcgaggcc
     1081 aagaagggga gcttcatccg catggaggcc aacaaggact actggggcgg agcgccagac
     1141 atcgatgagc tcatcattca ggtttacacg aaccaggaca cgatgaccat ggacctcaag
     1201 ggcaaggtca tcgacgccgc cgtcggtctg ccggtggggc agttcgagtc attgaagaag
     1261 gtcgacggca tcgccgccgt cgccgccagg cagaggtact tcaccgaact cgccatgaac
     1321 tgctacacgg gcccgtcgct cggcaatccc gtgctcaagg atgtgaactt ccgccgggcc
     1381 ataagctggt gcgtcgacaa ggagaagctc atcgatacct gctggggcgg ctacgccgac
     1441 cgtggagact ctatcctcgc tcccggaact gattacgcgt ggcagcccag cgagagtgaa
     1501 cgcttcggtt atgacttgga caaggccacg gagcttctcg cggaggccgg ctaccgggat
     1561 accgacggcg accagatcct gaacgacctc cataacgcga acagggacat caagctgcgg
     1621 ctgtggacgc gcgaggagtc tcccgagcag cagcgcgctg gaaagctcat cgccggggct
     1681 cttgagtcgg tgggcatcga catcgaactg agcgtgatga acgacggggc gatcagcgac
     1741 ggtatctaca actacgagga cggcacttac aagccgaact tcgacatgtt catatggggc
     1801 tggcaaggaa gtgccgatcc ggactaccag ttggccgact tcgtcaccga ccagatcgag
     1861 atgtggaacg actgctgctg gtcgaacgag cggtacgaca agctacgggc ccaggaatcg
     1921 accgagaccg accccgccag gcgcgagcag gaagtcgtgg agatgcagaa gatcttctac
     1981 gagtcggccc cttacgtcgt catcgcttac ccgcggaagc tcgtggccta cgactgcgac
     2041 cagtgggaag gttgggtccc gtacccaggc aaggagggcg acccggtctt ctcgtacgac
     2101 aacgtcgaca cgtacgtgaa acttgcggtg aagaccgggt ccgagtcagg cggcgggtcg
     2161 ggcggcatcg tggcagtcgt catcgtcgtc gtgatagtcg cggcgatcgt ggtcttcata
     2221 ctcgtgcggc gcaggacgag ggaagcggaa gaggaatgag agcgcgctgg gcgcggcacg
     2281 cccagcgcgc ccccgccgga agcgctcgcg tctacctggg cagcttctcg cgcagcgcct
     2341 gtcacgcgaa caggtcgagg agacagacgc ccacgcgttt ccttgacaga ttgagtccgg
     2401 cgcacaacat gggagaggcc tcgtggtcgc cgcttgcgtg gtgacgcacg gagcatgccg
     2461 tcacggaggc ctctcttcat ggcattcagg ctggggaatc acgtgatcgt cgacaacccc
     2521 ttcggctgga tgaagacggt cggccgcctt gccaagctac gccaccgcgg caaggccaag
     2581 gccgtagacc tccacacgtt cgcctgtgcc gccttcaacc tggtgcgctt gcgaactctg
     2641 ctggcggagc cgtcttcggc gtgaggtaaa gagtcaggaa ttgaccctga cggccccgtt
     2701 tgggggctcg cgcggggccg gacttgcctg catctcgcct tctgtcactc caggcagcaa
     2761 acgtacccct cccacggcgg ccttcttcag cagcctgtta tgcccgagca cagcgcgagg
     2821 gcggcgggcc gtcaagcccg ccgccctctg cacgtttcgc cagagcggcg ccgtgccgca
     2881 tgcggcacgg cgccgctctg gcgtgagtcc cgtcagccgg cgagcgtggc gcgcgcctcc
     2941 tcgagcaggc gccgcggatc cggccactca ccgaggccga agtcgcgcgc ggcgcgatcg
     3001 atgaccgagc agagcgtctc ctcaaa
//
LOCUS       DAOSPL010000454         3023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_32549_length_3023_cov_3.189690, whole genome shotgun sequence.
ACCESSION   DAOSPL010000454 DAOSPL010000000
VERSION     DAOSPL010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32549_length_3023_cov_3.189690"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2033
                     /locus_tag="PLQ54_09220"
     CDS             <1..2033
                     /locus_tag="PLQ54_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOS93480.1"
                     /translation="YMTSTTLAERTTQLSNIDKFAGLFDLGAQTLTIYNASGESANVV
                     LNGSDTLQGAAEKIRDAIIKPVAQGGLGMGVDGDLSANGVDGNVAVFVSDAVENSDEA
                     VAGTIVVRSTVAGSQGRLAFSGDQKLLDALSIAQIRDPNDPLEVTVTDAHTGEIIGKT
                     IVDDNMVRNIIQGVDLKIDSRVDTVVRYVPAGVSLVTGDILEGGSAGATASATTDILA
                     GADTFVQGNFTAFQGVEPDSGPAGGANVRADYELRTLTAADQFSVADFRFMAEAGADK
                     TGFALSAPTISANTAAGYFELEVLGIDSDADGVLETTAGTGGTLTNADANGSGGIQLA
                     LRKYDSAGTLLEEATVTLGDDTANGTAVDNVALNLFETAIGGVANSGLSAANAVAAAD
                     TANITLGNGATYYVGDKITFAIANGSGTNMAVLHNDKTDAGFANVTAYGDDTNYHANI
                     AGLGANTTSAGIRVGLATQLGAYSTLAGSTVDATIGYYDSSGNFVVGGGSVTLGQTGM
                     TVQSGTVRFSLTQETSTGPGFVFESAPGAAVEYLHLVDNRITLQIGANAGQTLTTSIA
                     AMDTTALGVNDMLVISRDLAAEAISKADTAINLVSSERAKLGAYVNRLEHTMNVLDIQ
                     SENLLAAESRIRDLDMAKETIAFTRNQILMQSGIALLAQANALPQNVLSLLR"
     gene            2195..2575
                     /locus_tag="PLQ54_09225"
     CDS             2195..2575
                     /locus_tag="PLQ54_09225"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HOS93481.1"
                     /translation="MAAGDLGRAAAVDVAQRASATQAAEWADRATHPESAGDAAQEKP
                     EADPEDFWSDREVRRAADDFNRAFTLFDIQAKFSVHEASGSIIVDVIDTRTGDVIREI
                     PPRELLDRWAQMMDIMGLFVDRRA"
     gene            2717..>3023
                     /locus_tag="PLQ54_09230"
     CDS             2717..>3023
                     /locus_tag="PLQ54_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93482.1"
                     /translation="MSATAEAAEAAAVTEPIASQDVGVLDEVTRRVQEQYRQYPYPRF
                     AEESIVDGRRLVSVDYLRYMQIAAWPKRRNLSGLRVLDAGGGTGQVLTQIAECHPEVQ
                     "
BASE COUNT          532 a          957 c         1067 g          467 t
ORIGIN      
        1 tctacatgac gagcaccacc ctggccgagc gcacgaccca gctcagtaac atcgacaagt
       61 tcgccggtct gtttgatctt ggcgctcaga ccctgaccat ctacaacgca tcgggcgaga
      121 gtgccaacgt ggttctgaac ggcagcgaca cgctccaggg cgcggcggag aagatccgcg
      181 acgccatcat caagcccgtg gcccagggcg gcctgggaat gggcgtggat ggtgacctta
      241 gcgccaatgg cgtcgatggc aatgtggccg tgttcgtctc cgacgcggtc gagaacagcg
      301 atgaagccgt ggcgggcact atcgtggtcc gctcgacggt ggcgggcagc cagggtcgcc
      361 tcgcgttctc tggcgaccag aagctgctgg atgccctgag catcgcgcag atccgggacc
      421 ccaacgaccc gctggaggtc acggtgaccg atgcccacac gggcgagatc atcggcaaga
      481 cgatcgtgga tgacaacatg gttcgtaaca tcatccaggg cgtcgacctg aagattgaca
      541 gccgggtgga cacggtggtg cgctacgtcc cggccggtgt ctccctggtc acgggcgaca
      601 tcctcgaggg tggatccgcc ggcgcgacgg ccagtgcgac gacggacatc ctggcgggcg
      661 ccgacacgtt cgtgcagggt aacttcaccg ccttccaggg cgtcgagccc gactcgggcc
      721 cggcgggcgg cgcgaatgtc cgggccgact acgagttgcg gacgctgacc gcggccgacc
      781 agttctcggt ggcggacttc cgcttcatgg cggaggcggg cgccgacaag accggcttcg
      841 cgctgtcggc accaacgatc tcggccaaca ccgccgccgg ctacttcgag ctcgaggttc
      901 ttggcatcga ctcggacgcc gacggcgtgc tcgagaccac ggcaggtacg ggcggcacgt
      961 tgaccaacgc tgatgccaac ggatcgggag gcatccagct cgcgctgcgc aagtacgaca
     1021 gcgcgggcac gctgctggag gaagccaccg tgacgttggg cgatgacacg gccaacggca
     1081 cggcggtcga caacgtcgcg ctgaacctgt tcgagactgc catcggtggt gttgccaaca
     1141 gcggtctgag cgcggccaat gccgtggcgg cggccgacac ggcgaacatc actctcggca
     1201 acggcgcgac gtactatgtg ggggacaaga tcacgttcgc catcgcgaac ggcagcggga
     1261 cgaacatggc ggtccttcac aacgacaaga ccgacgccgg cttcgccaac gtcaccgcct
     1321 acggcgacga cacgaactac cacgcgaaca tcgccggtct gggcgcgaac acgaccagcg
     1381 cggggattcg cgtcggcctg gccacccagc tcggcgcgta ctcgactctg gcggggtcga
     1441 cggtggatgc aaccatcggc tactacgatt cgtcgggcaa cttcgtggtc ggtggcggat
     1501 cggtgacgct gggccagacg ggcatgacgg tgcagtccgg cacggtgagg ttctcgctga
     1561 cccaggagac ctcgaccggg cccgggttcg tcttcgagtc ggcgccgggt gcggccgtcg
     1621 agtacctgca cctggtggac aaccggatca cgctccagat cggggcgaat gccggccaga
     1681 cgttgaccac gagcatcgcg gccatggata ccacggccct gggcgtgaac gacatgctgg
     1741 tcatcagccg tgacctggcg gccgaagcga tcagcaaggc cgacacggcc atcaacctgg
     1801 tgtccagcga gcgggccaag ctcggagcct acgtcaaccg gctcgagcac accatgaacg
     1861 tgttggacat ccagtcggag aacctgctgg ctgccgagag ccggatccgt gacctcgaca
     1921 tggcgaaaga gaccatcgcc ttcacgcgga accagattct catgcagtcg ggcatcgctc
     1981 tcctggctca ggccaatgct ctgccgcaga acgtgctgtc cctgttgcgg tagtcgtgcg
     2041 gcgggcggcg gcctgctctg ggcctgctgg gcccaagtgg gccgccgccg cttcgctcgt
     2101 cggcacgtaa cggagcactg gtggctcggg atgctgtgaa ccgatggtgc tccggagcgg
     2161 gcggcgggcg gtgcggccac aggaggtggt gggtatggct gcgggcgatc tggggcgggc
     2221 tgcggccgtc gacgtggctc agcgagcgtc ggcaacgcaa gccgcggagt gggcagaccg
     2281 tgcgacccac ccggagagcg ctggtgatgc cgcacaggag aagcccgagg cggatccgga
     2341 ggacttctgg agcgatcggg aagtccggcg ggctgccgac gatttcaacc gggcgttcac
     2401 cctgttcgac atccaggcca agttctcggt ccatgaggcc agtggctcca tcatcgtgga
     2461 cgtgattgac acgcggaccg gtgacgtgat ccgcgagatc ccgccgcggg agcttctcga
     2521 ccggtgggca cagatgatgg atatcatggg gctcttcgtc gacagacggg cctagctcgg
     2581 tgccccgaga aggcggacgg gcgctccttc gcgtgaggcg gaggggcgcc cgttgctgtc
     2641 tgtcgcgctc tctcgctaag gccccggcgg tctgggaccg aagacccaac cggagttgcc
     2701 ggagcggagg gatcgcatgt cggcgacagc ggaggcggcg gaggccgccg ccgtgaccga
     2761 gcctatcgcg tcccaagacg tgggcgtcct ggacgaggtc acccggcgcg ttcaggagca
     2821 gtaccgccag tacccatatc cccggtttgc cgaggagagc atcgtcgatg gacggcgcct
     2881 ggtctcggtg gactatctgc gctacatgca gatcgcggcg tggccgaagc gccgcaacct
     2941 gtcgggtttg cgcgtgctgg acgcgggagg cggcacaggg caagtgctga cgcagatagc
     3001 cgagtgtcac cccgaggtcc aac
//
LOCUS       DAOSPL010000455         3017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_32635_length_3017_cov_2.424375, whole genome shotgun sequence.
ACCESSION   DAOSPL010000455 DAOSPL010000000
VERSION     DAOSPL010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32635_length_3017_cov_2.424375"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..718)
                     /locus_tag="PLQ54_09235"
     CDS             complement(50..718)
                     /locus_tag="PLQ54_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93483.1"
                     /translation="MARVPHRLEWVVLLLGATIILSSCKTETKSPVPPGKLPSGAPAP
                     VRNTAEAPEGGWCVEDGAGSRLVSLEPLGDGFNLCGPEGKSQGQVVVKDDKVELMEQK
                     QLALKVKAKDDGCKLHDGQDQVLIEVKRKDNGYLVKDAADKEIGRIKAKDAGAYKIYD
                     AGGAEIGKIKPKDSGLELEDPSDKRLYRVKGKLPARAGAFWGIHGLSDQQKVALSVYF
                     SKWE"
     gene            865..1833
                     /locus_tag="PLQ54_09240"
     CDS             865..1833
                     /locus_tag="PLQ54_09240"
                     /inference="COORDINATES: protein motif:HMM:NF027464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HOS93484.1"
                     /translation="MEPGKPDAEARPQPLDPNVPGPGRRVLRIALAAAVVAVGACAFF
                     QWAGVAPREVARTFPNGYPRRSADYAWHRHTRSYTPIASRIGIPAGFHEVPVEPGSWP
                     EWLRFLPLMPAHSPIRGADGRPLGWPGIPAPPGVVDMDVRRNQECADTILRLRAEYLR
                     WAGHDDRLAFSLAGGGTISWADWRRGMRPVPVGGRVRMVRMAGPSESRQSFEAFLDAV
                     FNWCNTDSLSGDGIPATPEDLRIGDFVSRPGAGARGHAMMIVDLAVDADGNRRALILQ
                     GAMPAQSAHLLRASTGSAWIPIYPAPCFSVPGWREFTWDLLRRFDP"
     gene            complement(2620..2988)
                     /locus_tag="PLQ54_09245"
                     /pseudo
     CDS             complement(2620..2988)
                     /locus_tag="PLQ54_09245"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
BASE COUNT          434 a         1159 c          940 g          484 t
ORIGIN      
        1 gtgcttgatc agaaaccccg acacaacctc gcagcctctg gccatggccc tactcccact
       61 tggagaagta gacgctcagc gcgaccttct gctgatcgga tagcccatgg atgccccaga
      121 acgcgccggc ccgggcgggg agcttcccct tgacgcgata gagtcgcttg tctgacgggt
      181 cctcgagctc caacccactg tccttgggct tgatcttgcc aatctccgcg ccgccggcgt
      241 catagatctt gtatgcaccc gcgtccttgg ccttgatgcg cccgatctcc ttgtccgccg
      301 cgtccttcac gaggtagccg ttgtccttgc gcttcacctc aatgaggacc tgatcctgac
      361 catcatggag cttgcagccg tcatccttgg ctttcacctt cagagcgagc tgcttctgct
      421 ccatgagctc cactttgtca tccttgacca cgacttggcc ctgcgatttg ccttcgggcc
      481 cgcagaggtt gaacccgtca cccagaggtt cgagcgatac cagacgcgaa cccgcaccat
      541 cctcgacgca ccaaccgccc tccggggcct cagccgtgtt cctcacgggc gcgggcgcgc
      601 ccgaaggcaa cttcccgggg ggcaccggcg atttggtctc ggtcttgcac gatgacagga
      661 tgatggtagc accgagcagc aggacgaccc attcgagacg gtgcggcact ctggccacgg
      721 tgactccccc taatcgcacg cccgcacgcc gcccgtgccg ggcaatgcgg tgcgactggc
      781 acgtcctttc gctgccgagg ggcaccgtcc ctccggccag cgagctgttg acccatcgga
      841 gaccggcccc gcacaatgcc gcccatggaa ccgggcaagc ccgatgccga ggcacgtccg
      901 cagccgctcg atccgaacgt accgggcccc ggccggcggg tattgcgcat cgccctcgcc
      961 gccgcagttg tggcggtcgg cgcatgcgcg ttcttccagt gggccggtgt cgcgccccgt
     1021 gaggtggctc gcacgttccc gaacggctac ccgcgccgct cggccgacta tgcctggcac
     1081 cgtcacacca ggagctacac gcccatcgcg agccgaatcg gcatccccgc gggcttccat
     1141 gaagtgcccg tcgagcccgg gtcgtggccg gagtggctgc gcttcctccc gctcatgccc
     1201 gcccactcgc cgattcgagg cgccgacggc aggccgctgg ggtggcccgg catacccgcc
     1261 ccacccggag tggtcgacat ggacgtccgg cgcaaccagg aatgcgccga cacgatactc
     1321 cggctccgtg ccgagtactt gcgctgggcc gggcacgacg atcgcctggc cttctcgctg
     1381 gccggtggag gcacgatctc gtgggctgac tggcggcgcg gcatgcgccc ggtgcccgtg
     1441 ggcggccggg tgcggatggt gaggatggcg ggtccatccg agtcgcgcca gtccttcgag
     1501 gcgtttctgg acgccgtctt caactggtgt aacacggact cactgagcgg cgatggcatc
     1561 cctgcgacgc ccgaggacct ccggatcggc gacttcgtct cacgccccgg cgccggcgcc
     1621 cggggccacg ccatgatgat tgtcgacctg gcggtcgacg ccgatgggaa tcggcgagcc
     1681 ctgatcttgc agggggccat gccggcccag tccgcccacc ttctccgcgc cagtaccggc
     1741 agcgcgtgga tccccatcta cccggcgccc tgcttctccg tccccggctg gcgcgagttc
     1801 acgtgggacc tcctgcggcg tttcgacccc tgaggcaagc ccgggcagca gggcgagcga
     1861 cccggctagc tccggggcgg cctcacggtc ttccgccgac cccaggccgg ggcgatcggg
     1921 gtgcgaaccc ggtgaccgaa gggccgcgac gaggtccggc agccggtcga ggactgcctg
     1981 cagaaccagt gctgcgccgc actcctccag cgcctgagcg cggatgcgga ggctgtccgc
     2041 ttccgcctga gcgcgcgcgc gaagcgcatc ggcctccgca agccccctga gccggatcgt
     2101 ctcagcctca gccgcacctc gctgcacgat ggcctcggcc tccgccgctc cgtccaggcg
     2161 tctggtgtcg gcccgagccc ccgccttggc ccggatccgg tacgcctcgg catcggccag
     2221 ggactccacg cgctggcgct cggcggccgt cggctgtctc acccggtgct ccagctcgcg
     2281 ctccttgcgt tggatctcga gctcctcgag ccggatccgc tcctcgcgct ccaccaggtc
     2341 gataccgacc cgttcgcgcg cgatgtcctg ccgggccttg gcatcagcca gatcgtagct
     2401 cgagtcacgg accgcacggg ccccctcgac acgcccggcc agctcgtgct tctccatgtc
     2461 cccatcgtgg cgcgcgcgga tgatggcccg ctcggcttcg atccgcgaga cctccgcctc
     2521 ccggcgagac cgcgccgccg ccgccacggc ggccccctcc gctccagctt cgcccaccgc
     2581 agcgtcccgc tcagcctcgg ccgcactcac cctcgcgacc gagagcaggt accccgacgg
     2641 gtcaatcacc tcatccaccg agagacctac caggtccagc cccaccaccg ccaggtcggc
     2701 ggccaccagc ggtgtcacgg agtctcggac accatcggga tgggcgtgaa tctcctcaat
     2761 gctcatcgaa ccacaggcgg cgcgcacgcg cccgagcacc acattccgga cgatggcggc
     2821 gcgctcggcg gccgtcttgc cgagcagcat ctcgatggcc aggcccaggc gcacctcgtc
     2881 gtcgaccgcc acccgcgccc acgcaccaca gcgcagcgcc accggcaccc cgtgcttcgt
     2941 cggataggtg ggtgtcgaga catccaccgc aaacaggcca acgggcatgc ggtaggccct
     3001 ctcgcgaatg ggccata
//
LOCUS       DAOSPL010000456         3006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_32784_length_3006_cov_3.635378, whole genome shotgun sequence.
ACCESSION   DAOSPL010000456 DAOSPL010000000
VERSION     DAOSPL010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32784_length_3006_cov_3.635378"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2444)
                     /locus_tag="PLQ54_09250"
     CDS             complement(<1..2444)
                     /locus_tag="PLQ54_09250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOS93485.1"
                     /translation="MSDSYDMSAYMDVFMAEADEQLALLEESLVDLEKEATPDLLQRL
                     FRAAHTLKGSSAAMGFTRMAELTHAMENVLDELRNERIGVTSAIVDVLLAGLDSLSAM
                     RRGLEHGAEPDLDTTALTAQLRDITRAARPGAGAAVEAPPVGIQLEAEELRAIERARQ
                     AGATAYEITVCLKPGCPLKAIRAALVLATVRKQAELVKVMPPEEQIDREEFGDDFRMI
                     ATTTDAPDRIVAAVSALSEIGSVTIQEMPETVAAAEERPSEAGLAAETDLVFLDHELD
                     AVEMALGQGFSVFVVDVGLRPGTPLKGLRATMAVSTAEKIGDVIRTDPGPDDIEEERF
                     GDSFRMVLITKEPAAAVRAQVESLGEIESATVTPVALPKAEPQAGAAPPSALAPAAVA
                     PIEAPAEPGGVRRQTTVRVNVEQLDNLMNLVAELVIDRTVLEQVLLDLTAQSGKQSDV
                     ASSLEEAVTRIGRRTSDLQDEIMKARMQPVDTVFSRFPRMLRDLAHKAGKRIDFQIVG
                     GETELDRSVLEVIGDPLIHMLRNSVDHGIELPEERKAAGKPEVGSIVLSARHEENYIV
                     IQVDDDGRGIDPARVRQRAVERGLLSREAADRMSDREAMDLIWAPGFSTAEKVSDVSG
                     RGVGMDIVRNNVERLNGRIDVQSIVGQGTSMSVRLPLTLAIIQALLVRAQGRVYALGL
                     TCVQETERRQLSEIQTIQGAETVVLRGRVLPLIRFDWCYHYTRPAPATGARNGRIFIV
                     VVRVGEREAGIVVDELIGEQEIVIKSLGSFIGDVRGVSGATILGDGTVALIVDAAALL
                     DKAADERSQPIAALEAA"
     gene            complement(2517..>3006)
                     /locus_tag="PLQ54_09255"
     CDS             complement(2517..>3006)
                     /locus_tag="PLQ54_09255"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOS93486.1"
                     /translation="GMMQFVTFSLGHELYGVPVDVVDEVIHMQFVADVPGVPDYVEGI
                     TNLRGNVVPVVNLRWRLGLPPASGEGSDRLLVVEHNRERIGLEVDTVREVVDVAVTDI
                     EPPSPIVSSIDSDLIQGLAKTPSGFVILLDLDRTLGAPEASGGRQALGPGRAAAALPP
                     AQ"
BASE COUNT          503 a         1083 c          929 g          491 t
ORIGIN      
        1 gccgcctcaa gagccgcgat gggctggctc cgctcgtcgg ccgccttgtc gagcagggct
       61 gcggcatcca cgatgagagc caccgtgccg tcgcccagta tggtggcgcc cgagacgccg
      121 cgcacgtcgc cgatgaagct gcccaggctc ttgatcacga tctcctgctc gccgatgagc
      181 tcatccacga caatgccagc ctcccgctcg cccacccgga cgacgacgat gaagatccgg
      241 ccgttgcggg cgcctgtcgc gggggcagga cgggtatagt ggtagcacca gtcaaagcgg
      301 atcagcggca gaactcggcc acggagcacg accgtctcgg cgccctggat ggtctggatc
      361 tcgctcagct gacgccgctc ggtctcctgc acacacgtca ggcccagggc atagacccgt
      421 ccctgtgcgc ggaccagcag cgcctggatg atcgccagtg tcaggggaag gcgcacagac
      481 atcgaggtcc cttgccccac gatggactgg acgtcgatcc gtccgttcag gcgttcgacg
      541 ttgttacgca cgatgtccat gccaacccct cggcccgaga catcgctgac cttctccgcc
      601 gtgctgaacc caggagccca gatcaggtcc atcgcctctc ggtctgacat gcggtccgcc
      661 gcctcgcgcg acagcaggcc ccgctccacc gcgcgctgcc gcacgcgcgc cgggtcgatg
      721 ccccgcccat cgtcgtcgac ctggatgacg atgtagttct cctcgtgccg cgccgagagc
      781 acgatgctgc ccacctcggg ctttcccgcc gccttgcgct cctcgggcag ctcgatcccg
      841 tggtcgaccg agttccgcag catgtggatt agtggatcgc cgatcacttc gaggaccgac
      901 cgatcgagct cagtctcgcc gccaacgatc tggaagtcga tgcgcttccc ggccttgtgc
      961 gccagatcgc gcagcatccg cgggaaccgg ctgaagaccg tatcgaccgg ctgcatgcgc
     1021 gccttcatga tctcgtcctg gagatccgac gtacgacgtc cgatccgtgt gacggcctcc
     1081 tcaagcgagg aggccacgtc gctctgcttg ccgctctggg ccgtgagatc gagcaggacc
     1141 tgctcgagga ccgtgcggtc gatcaccagc tctgccacga ggttcatcag attgtccagt
     1201 tgctcgacat tgactcgaac cgtcgtctgc cggcggacgc caccaggctc agcgggcgct
     1261 tctatggggg ccacagccgc cggtgccagt gcggatggtg gcgccgcacc cgcctgcggc
     1321 tcggcctttg gcaacgccac gggagtgacc gtggccgact cgatctcgcc cagcgactct
     1381 acctgggccc gaaccgcggc cgccggctcc ttcgtgatca gcaccatgcg gaagctatcg
     1441 ccaaaccgct cctcctcgat gtcatcagga ccgggatccg tacggatgac gtcgccgatc
     1501 ttctctgccg tcgacaccgc catggtcgcg cgcaatccct tgagcggggt gcctggccgc
     1561 agccccacat ccacgacgaa caccgagaag ccctgcccca gcgccatctc gacggcatcc
     1621 agctcgtgat ccagaaacac caggtccgtc tcggcagcca agccagcctc ggacgggcgc
     1681 tcctccgcag ccgccaccgt ctcgggcatc tcctggatag tcaccgaccc gatctcagag
     1741 agggcactga cggctgccac gatgcggtcg ggtgcgtccg ttgtcgtcgc gatcatccgg
     1801 aagtcgtcgc cgaactcctc gcggtcgatc tgctcctcgg gcggcataac cttgaccagc
     1861 tcggcctgct tgcgcacggt ggcgaggacc agagcggccc ggatagcttt gagtgggcaa
     1921 ccgggcttga gacacacggt gatctcatag gccgtggcgc cggcctgacg agcccgttca
     1981 atggcacgga gctcctccgc ctcgagctga atgcctaccg gcggggcctc gaccgctgcg
     2041 cccgctccgg ggcgcgccgc tcgggtgatg tcgcgcagct gagccgtcag cgccgtggtg
     2101 tcgaggtccg gctcagcgcc gtgctccagc ccccgtctca tggcactcag ggagtccaga
     2161 ccggcgagca gcacgtccac aatggccgac gtgacgccaa tccgctcgtt gcggagctcg
     2221 tcgagcacgt tctccatggc gtgcgtgagc tcggccatgc gcgtgaaccc catcgccgcg
     2281 gaggagccct taagcgtgtg agcggcgcgg aacaaccgct ggagtagatc gggcgtggcc
     2341 tccttctcga ggtccacgag tgattcctca agcagagcca actgctcgtc ggcctctgcc
     2401 atgaacacgt ccatgtatgc gctcatgtcg tagctgtcgc tcattgccat cctccgcccg
     2461 gaacgggtac ccaccactcg agaccgctcg gcactcccaa caccggccgc cagagcctac
     2521 tgagcgggcg gcagtgcggc cgccgcccgg cctggcccga gagcctgccg gccgcccgaa
     2581 gcctccggcg cccccagggt ccggtcgaga tccaggagaa tcacgaaacc cgacggtgtc
     2641 ttcgccagac cctgaatcag gtctgagtcg atggaggaca cgatgggcga cggaggctcg
     2701 atgtcggtca cggccacgtc gacgacctcg cggacggtgt cgacctccag cccaatgcgc
     2761 tcccgattgt gctccacgac gagcaggcgg tcggaaccct cgccggacgc aggcggcaaa
     2821 cccagccgcc aacggaggtt cacgacgggc accacgttgc cccgaaggtt ggtgatgccc
     2881 tccacatagt cgggcacccc cggcacgtcg gccacgaact gcatgtggat cacttcgtcc
     2941 accacatcga cgggcacgcc atagagctcg tggccaagcg agaaggtaac aaactgcatc
     3001 atacct
//
LOCUS       DAOSPL010000457         2990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_33009_length_2990_cov_3.424532, whole genome shotgun sequence.
ACCESSION   DAOSPL010000457 DAOSPL010000000
VERSION     DAOSPL010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33009_length_2990_cov_3.424532"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2872)
                     /locus_tag="PLQ54_09260"
     CDS             complement(<1..2872)
                     /locus_tag="PLQ54_09260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOS93487.1"
                     /translation="MNSATASGGRAGLLAMGLGLVMVCSALADAAETRVVRDTDPHSG
                     LTVECVADEILVGFAPGMQADEIAALHAQFGPAATRRIFGTNIDVVTLTAQQTRAAAI
                     AFYARKPGVVGAELNLFARWHAVTPFTPNDPGYSQTYAFRLCNMDDAWGALAERSADP
                     ANPTIGGTAAILAILDSGFDLSHPDLASNYFTNPRETAGNGVDDDGNGFVDDVRGWDF
                     QDNDNDPSPQAGATSSHGTACAGCAAAIGNNGIGIPGMVWRCQLIPVRVGNDSGSSVA
                     SAIAGINYAAAMGADVISMSFGVAGTSALFTSAITTAAALGADSFGNNPDRAGINFVA
                     SAGNDNVDMTGTPYYPVGSETNNEIIGVASVTSTVTRSDFSNYGTGVDIAAPGSAIYT
                     TEMGSGYTTTQGTSFSAPYVAGYLTLLRALAPTLTANELRDILLESADADTLYSANPG
                     FKTGAKLGAGLLEGLVPVQIVTEAPPDVSIETPSNGERLFNITPTIRVSAARSFARGR
                     MLKQIVVEIDQERDPTGPNYPITRPGREAVVWTAAEPAVGEASASATYVVDPPLATGD
                     SGATLHTLRIRVVEVDGRATYYPPVGETWRFRIATRALPRGARMISFPLALGQGGATN
                     QNYARPREVLGAPVGRPGDAVIARWNPATGEYVRSDVAGEDDEYIRFFEPGKGYWVNS
                     PTNVNKLYLRGLDLNEEVEVRDAPAGTNAGALLAPGWHQIGNPFPFAIALNSSLVRTE
                     SGDLIPFEVAVTRRLVRGTIFQYLPETGEYSGTTIPDAVLSPMTGYWLRTMAPIRLFL
                     EPSEASVSGVSAASTRASAAVPNGWTVSLSASCGSAKDTTNAFGMAAKASSEYDEGLD
                     MEEPPPVSSDLHLSFVHSDWADSGCQLSRDVVGPADTATWPVQVRTRQANAQVTLSWG
                     DMRSVPREYSVYLVDSETGKIVSMRNASTYTFATGDTGCARQL"
BASE COUNT          474 a         1018 c          985 g          513 t
ORIGIN      
        1 cgagctgcct cgcacacccg gtgtcgcccg tggcgaacgt ataggtcgag gcgttccgca
       61 tgctcacaat cttcccagtc tcggagtcca ccagatagac gctgtactcc cgtgggaccg
      121 accgcatgtc gccccacgac agggtcactt gggcattggc ctgtcgggtc cggacctgga
      181 ctggccaggt agccgtatcg gcaggcccca cgacgtcgcg gctcagctgg cacccactgt
      241 cggcccagtc cgagtgtacg aaggagaggt ggaggtccga agagaccggc ggcggctcct
      301 ccatgtcgag gccctcatcg tattcagacg atgccttggc agccatgccg aaggcattag
      361 tcgtgtcctt ggcactgccg caggacgcgc tcagcgacac cgtccacccg ttcgggacgg
      421 cggccgacgc gcgcgtcgat gcggccgaga ctccggacac cgaagcctcc gacggctcca
      481 ggaacagccg aatgggagcc atggtcctca gccagtaccc ggtcatcggg gacagcaccg
      541 cgtcggggat ggtggtcccc gagtattccc cggtctcggg aagatactgg aagatggttc
      601 cgcggaccag ccggcgggtc acggctacct cgaagggaat caggtccccg ctctccgtac
      661 gaaccagcga ggagttcagg gcaatcgcga acgggaacgg gttaccgatc tgatgccagc
      721 ccggcgccag gagggccccg gcgttcgtcc ccgccggcgc atcgcgtacc tcgacctctt
      781 cgttcaggtc gagtcccctc aagtagagtt tgttcacgtt ggtcggggag ttgacccagt
      841 atcccttacc gggttcgaag aagcggatgt actcgtcgtc ttcccccgcc acgtcgctgc
      901 gcacgtactc gcctgtggcc ggattccaac gcgcgatcac ggcgtcaccc ggacggccca
      961 ctggcgcgcc aagcacctct cggggacgcg cgtagttctg gttcgtcgct ccgccctgtc
     1021 ccagcgccag ggggaaggat atcatccggg cccccctggg cagcgcccgc gtcgcgatgc
     1081 ggaagcgcca cgtctcccct acgggcggat agtaggtggc ccgcccatcc acctcgacca
     1141 cgcgaatgcg cagggtatgc agggtcgccc ctgagtcacc tgtggccaac ggcggatcga
     1201 ccacataggt agccgacgcg gacgcctcgc ccacggccgg ttcggcggcc gtccagacca
     1261 ccgcttcgcg cccgggcctc gtgatgggat agttggggcc cgtgggatcc cgctcctggt
     1321 cgatctcgac cacgatctgc ttgagcatcc gccccctggc gaatgaccga gcggccgaaa
     1381 cgcgaatcgt cggggtgatg ttgaagagcc gttcgccgtt cgagggcgtc tcgatactca
     1441 cgtcgggcgg cgcctcggtc acgatctgca ccggcacgag cccctcgagc aggcccgccc
     1501 ccagcttcgc gccggtcttg aaccctgggt tggcgctgta cagggtgtcg gcatctgcgg
     1561 attcgagcag gatgtcccgg agctcgttgg ccgtcagtgt cggggcgagg gctctgagga
     1621 gcgtcaggta gcccgccacg tatggcgccg agaatgaagt gccctgcgtg gtggtgtagc
     1681 cgctgcccat ctccgtcgtg tagatcgcgg agcccggggc agcaatgtcg acacccgtgc
     1741 cgtagttgga gaagtcggac cgagtgacgg tggacgtaac cgacgcgacg ccgatgatct
     1801 cgttgttcgt ctcggacccc accgggtagt atggtgtgcc ggtcatatcg acattgtcgt
     1861 tgccggcgga ggcgacgaag ttgatccccg cccgatctgg gttgttgccg aagctgtccg
     1921 cccccagcgc agcggccgtc gttatggcgc tcgtgaagag cgccgacgtc ccggccacgc
     1981 cgaagctcat gctgatcaca tcggcgccca tggcggccgc atagttgatg ccggcgatgg
     2041 cgctggccac cgaggacccg ctatcgttgc ccacccgaac tgggatgagc tggcatcgcc
     2101 agaccatccc gggaattcct attccgttgt tcccaatggc cgccgcgcac cccgcgcagg
     2161 ccgtgccgtg gctcgatgta gcgcccgcct gtggggacgg gtcattgtcg ttatcctgga
     2221 agtcccagcc cctgacgtcg tcgacgaagc cgttaccgtc gtcgtccacg ccgttgccgg
     2281 cggtctctct gggattcgtg aagtagttgc tggccaaatc cggatgcgac aggtcgaatc
     2341 ccgagtccag gatggccaga atggcggcag tccccccgat ggtgggattg gcggggtcgg
     2401 cggatcgctc cgccagggca ccccaggcat cgtccatgtt gcacagccgg aaggcgtagg
     2461 tctggctgta gccggggtca ttgggggtga agggcgttac ggcgtgccac ctggcgaaca
     2521 ggttcagttc ggcgcccacg acaccgggct tccgggcgta gaacgcgatg gcggccgctc
     2581 gagtctgctg ggccgtgagc gtcaccacgt cgatgttggt gccgaagatc cggcgggtgg
     2641 cggccggtcc aaactgggca tggagcgcgg cgatctcgtc ggcctgcatg cccggtgcga
     2701 agcccaccag gatctcgtcg gcgacgcact ccacggtgag tcccgagtgg gggtccgtgt
     2761 cccgcacgac ccgcgtctcc gcggcgtccg cgagtgccga gcacaccatg acaaggccca
     2821 gccccatcgc cagcaatccg gcgcgcccgc cgctcgccgt tgcgctgttc atagcccact
     2881 cacccttcac aacaacgaac ccgaacccag gccacctcgg cgtcgtgtca cgtggcgcca
     2941 tccattccct ctcacacaga cgcccccggg ccgccggccg tctcggcttc
//
LOCUS       DAOSPL010000458         2981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_33121_length_2981_cov_2.529733, whole genome shotgun sequence.
ACCESSION   DAOSPL010000458 DAOSPL010000000
VERSION     DAOSPL010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33121_length_2981_cov_2.529733"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1132)
                     /locus_tag="PLQ54_09265"
     CDS             complement(5..1132)
                     /locus_tag="PLQ54_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93488.1"
                     /translation="MAASASLVLMLTGGAPTCTAAGAPPVRVVFCSMADVGRAAPLGD
                     GCAIVVPLSISGQSYVAPAPRELVPAAEAAAVDQWRVTLASAHAHGARVLGSVDTLRW
                     GDVALPAGVQAAHPDWFETRPPGSPDEPGDAGRYLSVWHPATALVMRSLASDLWTAVP
                     DLDGLVLRHCYSHTEWVGFSESTRAACVRELRQDPLDLVRLGGASTDPKLRIPLMRVA
                     AWRQSAVDAALRAFVGAYRAVKPGAEIWAWGRPETYLMDPLERAQACEDWLGWLADGA
                     VDGVFLEGPWQGPGGAAGPPVELYRMVRDLVARIAPAPQVVPTIPTDDAGADVDLVAQ
                     LLRLEAQGPVPALGVVVKQTQRLADALELLRTFAPYTWPPG"
     gene            complement(1191..2678)
                     /locus_tag="PLQ54_09270"
     CDS             complement(1191..2678)
                     /locus_tag="PLQ54_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93489.1"
                     /translation="MRATAASVVAFVYCALVSTGAAPPDGWGPYWPDWAQKEVELAAC
                     GGDVAAALEWVSGWSGVPCLVPVVGDEGGERPEWLSQPADLRLPCDTLRGIVGWVRPH
                     GYYGITTRHFVVVVPEGPRPWGLTSTRGRDGVGASVMALACATAPLEVATRLLSGSRV
                     LAQQLTEEAQAALERTCVLNPRGRGLQHSLEGPLGISVGLTPTVEILLPDGTGRTIEL
                     GAVLRERNPLVGRALGVPKRIRLDAASGQAAVADWGPDRFRADFLGEPPEEIADSVRV
                     YQTDWLLARERLQALQELPVDLGGTMRVGDVCQRFTACRVDPGPFVENHVWASSGKAP
                     GGSALEGLAFAIGAWIQPEGEGLRLAEPQGRAVPEPLKLAARWASAQLPHREDLKLVC
                     LALEDRPAKPFGELEAEPRDLLRACLAAQEHPPAVVDATPVRLGLNLEFGLEPLRLTR
                     DDSTAAAEGEPVVLDVSHRRDATLSLVGRDCPCVSVDPYVLDLEG"
     gene            complement(2712..>2981)
                     /locus_tag="PLQ54_09275"
     CDS             complement(2712..>2981)
                     /locus_tag="PLQ54_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93490.1"
                     /translation="DCVFHLWMCRYAQVPTNWFGVFAPPQSQTWEETVRQQWFGKHTE
                     TVGSYDAQAHHMWRPGTPPTPVGVCNAYDNHDRAWSWAIVSEPCP"
BASE COUNT          485 a         1094 c         1008 g          394 t
ORIGIN      
        1 atgctcaccc gggtggccat gtgtagggag cgaaggtgcg aaggagctcg agggcgtcgg
       61 caaggcgctg cgtctgcttg accacgaccc ccaaagcggg gaccggtccc tgggcctcca
      121 gtctgaggag ttgggcgacc agatcgacat cggcaccggc atcgtcggtc ggaatggtcg
      181 gaaccacctg cggtgccgga gcgatgcgcg ccacgaggtc tcgaaccatg cggtagagct
      241 ccaccggcgg gccggccgcg ccgccaggcc cctgccaggg cccttcgagg aacacgccgt
      301 cgaccgcgcc gtcggcgagc cagcccaacc agtcctcaca ggcctgagcc cgctcgaggg
      361 ggtccatcag gtaggtctcc ggccggcccc acgcccagat ctcggcaccg ggcttgacgg
      421 cgcggtacgc accaacaaac gcgcgcaggg ccgcgtccac cgcgctttgt cgccatgcag
      481 ccacgcgcat gagagggata cgcagcttcg gatcggtact ggcgccgccc agtcgaacca
      541 agtcgagcgg gtcctgccgg agctcgcgca cacacgctgc ccgggtcgat tctgagaagc
      601 cgacccactc cgtgtggctg tagcagtggc ggagcacgag cccgtcgagg tcgggcaccg
      661 cggtccacag gtcactggcc agactgcgca tgaccaaagc cgtggccggg tgccacacgg
      721 acaggtacct gcccgcgtca cccggctcgt caggggagcc tggcggcctc gtctcgaacc
      781 agtccgggtg cgcggcttgc acccccgcag ggagtgccac atcgccccag cggagtgtgt
      841 cgacgctccc cagcacgcgc gctccatgcg cgtgggcgct cgccagagtg acgcgccact
      901 gatcgactgc cgctgcctca gcggcgggaa cgagctcccg aggcgcgggc gccacatacg
      961 actgccccga gatagacagc gggacgacta tggcgcagcc gtcgccgagg ggcgcagctc
     1021 ggccgacgtc agccattgag cagaacacga cgcggacagg cggcgcgccc gctgcggtac
     1081 aggtcggcgc gccgcccgtc agcatcagca ccagggatgc agaagcggcc aagctgccta
     1141 ctcggtgtcc ccgcagtgcg cgccagcctt cgtcgccagg cccgggcacg ctacccctcc
     1201 aggtcgagca cgtacggatc gaccgagacg cacgggcagt cgcgcccgac caaggagagg
     1261 gtggcgtcgc ggcggtggct gacgtcgaga acaacaggct cgccctcggc tgccgccgtg
     1321 ctgtcgtcgc gagttaggcg cagtggctcc agcccgaact cgaggttcag cccaagccgc
     1381 accggcgtgg cgtcgacaac ggccggggga tgctcttggg ccgccaggca ggcacggagc
     1441 aggtcccgcg gctcggcctc aagctcaccg aatggcttcg ccggccggtc ctccagggcg
     1501 aggcagacca acttcaggtc ctcccgatgg ggcagttgcg cgctcgccca ccgagccgcg
     1561 agcttcagcg gctctggcac cgcgcgcccc tgtggctcgg ccagtcgaag cccttcgccc
     1621 tcaggctgga tccacgcccc gatcgcgaaa gccagcccct ccagggccga cccgcccgga
     1681 gccttgccgg aactcgccca cacgtggttc tcgacgaacg ggcccggatc gacccggcat
     1741 gccgtgaagc gctggcacac gtcgccgacc ctcatggtgc caccgaggtc gaccggtagc
     1801 tcctggagcg cctggagccg ctctcgggcg agcaaccagt ctgtctgata gaccctgaca
     1861 ctgtcggcga tctcctcggg gggctcgccc aggaagtcgg cccggaaccg gtccggcccc
     1921 cagtcggcga cggccgcctg ccccgacgct gcgtccagcc tgatgcgctt gggcacgccc
     1981 aacgcgcggc ccaccagagg gttccgctcg cgaagcaccg cgccgagttc gatggtccgg
     2041 ccggtgccgt ccggcaggag gatctcgacg gtgggcgtga ggcccaccga gatcccgagg
     2101 ggtccctcga gggagtgctg gaggccccgg ccgcgcgggt tgaggacgca cgtgcgctcc
     2161 agcgcggcct gggcctcttc ggtcagttgc tgggccaaca ctcgcgaccc ggagagcagc
     2221 cgtgtggcga cctcgagggg tgctgtcgca catgccagcg ccatcacgct ggcgccaaca
     2281 ccgtcgcgcc ctcgggtgct cgtgagcccc cacggccggg gcccttcggg gacgaccacc
     2341 acaaagtggc gtgtggtgat cccgtaatag ccatgagggc gcacccagcc cacgatgccc
     2401 cggagcgtat cgcacgggag acggagatcc gcgggctggc tcagccactc aggccgctcg
     2461 ccgccctcgt cacccaccac aggcaccagg cacggcacgc ccgaccagcc cgacacccac
     2521 tcgagagccg cagccacgtc cccgccacat gcggcaagct cgacctcttt ctgggcccag
     2581 tcgggccagt acgggcccca gccatctggc ggcgcagccc cagtgcttac gagcgcgcag
     2641 tacacgaatg ccaccacgct agcggccgta gctcgcacag ggccacctcc cgtcgcgtgt
     2701 gccgacccga actacgggca gggctcactc acaatggccc acgaccaggc cctgtcgtgg
     2761 ttgtcgtacg cgttgcagac gccaacgggc gtaggcggcg tgccgggacg ccacatgtgg
     2821 tgcgcctgcg cgtcgtatga gccgaccgtc tcggtgtgct tgccaaacca ctgctggcga
     2881 accgtttcct cccacgtctg cgactgcgga ggagcaaaga cgccgaacca gttcgtgggt
     2941 acctgtgcgt agcggcacat ccataggtgg aatacgcagt c
//
LOCUS       DAOSPL010000459         2980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_33132_length_2980_cov_3.184274, whole genome shotgun sequence.
ACCESSION   DAOSPL010000459 DAOSPL010000000
VERSION     DAOSPL010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33132_length_2980_cov_3.184274"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..2897
                     /locus_tag="PLQ54_09280"
     CDS             24..2897
                     /locus_tag="PLQ54_09280"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOS93491.1"
                     /translation="MPSLDFIVGPRPRACTAVREAALELLGNGLNPAHLVLVALREDA
                     AERLARALADQAANLPRVVTPTSLAREILRRQGRAQLRRALGDLEEQLVLAHVIANSA
                     ELTGPFAHPAVRSSQGFAQDVGWLITQLKQLRIDPPAFDRLIQGLPDERALKPLAAIY
                     RAYAEHLARAEAADYRGAVWLALEALESDGDLLAQLRGQWRHMLIDGAEDLSPLEWEL
                     LRAVGADADVRAWADPAQSIFGFRGAVREPWELLRGAGARPLFHQAAPVEPPAVSFAA
                     FARFEDEAAWVAGKLRELAHAGAPAAVIVRSRTEAGRFEAALVRHGAPLEATASGAGA
                     WLMTRFIRDSLELLARGPRLLSERRMPRAEAQRVNDALMHLLGYCRSGGDALTVLGTV
                     AASARRAGRLLLHPPAGRRSAGAAEDTAVRWARDWYLAHFPAECEDIPRTILRLAAEL
                     RPGWQCDSHATHEAMRTLGTFLRRAGRLQETFQRVFGTSVPVQEILDQLADLVGRDGA
                     STQATIAVLTCHEAKGREFHTVFLPGLAEGVFPQTSRPPRLISQRAARALCDRVPGLS
                     GLLERPEEALAEERRAFHVARSCAVERLLLSCHLERGGQIVTPSRFLIEALPSGVGRA
                     ECALCHRRAPAAEPADCGACPVDPCPGRESRHTDGPWIVPVETRPAATVGPPVMAGHE
                     NLPLSASALKTYAKCPRRFFFERLLRVRDEGGDAATYGTIAHEALRRISQWAPEEREP
                     ARAGAEVRAVIEEHAEDFSSPAHRRQCEHELTDAVNQYLNHTESYARWRTIAAEAGVE
                     VELTDDTGQPHAFVGRLDLIAEAATGVRALVDFKTATGLAKTPDTIRKWFYVRDGEKP
                     GAERDYQLALYALAPQAREGVEQLAIQPIWPEYEGPPSPRVIEIGPAPGKGVLSLEEL
                     DDISRQLANLAREIKGREWFEPNPKACRNCPCLEVCDGGAEAE"
BASE COUNT          453 a         1034 c         1049 g          444 t
ORIGIN      
        1 tggcccagga ggcgacgcgc tctatgccct cactcgactt catcgtgggc ccgcgtccgc
       61 gtgcctgtac cgccgtgcgc gaggccgcgc tggaactact cggcaacggc ctcaacccgg
      121 cccatctggt gctcgtggct ctgagagagg atgcagccga gcggctggct cgagctctgg
      181 ccgaccaggc tgcgaacctg ccccgtgtcg tcacaccgac aagcttggcc cgggagattc
      241 tccggcgtca gggccgcgcc cagttgcgcc gggcgctcgg agacttggag gaacaactcg
      301 tcctggccca tgtcatcgcc aactccgccg aactgacagg gcctttcgcg cacccggccg
      361 tgcggtcgag tcagggcttc gcgcaggatg tagggtggct catcacacag ctcaagcagc
      421 tccgcatcga cccaccggcc ttcgatcggc tgatccaggg cctgcccgac gagcgcgcgc
      481 tgaagccgct ggccgcgatc taccgcgcat atgccgagca cctggcccga gccgaggcag
      541 cggactatcg cggcgcggta tggctggctc tggaggctct cgaaagcgat ggggacctgc
      601 tggcacagct caggggccag tggcggcaca tgttgatcga cggcgcggag gacctgagcc
      661 ccctcgagtg ggagctactg cgcgctgtcg gcgccgacgc cgacgtgcga gcctgggccg
      721 atcctgcgca gtcgatcttc gggttccggg gcgcggttcg agagccctgg gagctactcc
      781 gcggcgccgg ggccaggcct ctcttccacc aggcggcacc cgtcgagccg ccggccgtct
      841 ccttcgctgc gttcgctcgg ttcgaggacg aggcggcttg ggtagcaggc aagctgcgtg
      901 agttggccca cgccggggcg ccggccgcgg tcatcgtccg atcacggacc gaggccggcc
      961 gcttcgaggc ggccctggtc cgccacgggg ctccgctcga ggccacggcc tcgggcgccg
     1021 gagcatggct gatgacgcgg ttcatccgtg actcactcga gttgctggca cgcggtccga
     1081 ggctcctttc agagagacgc atgccccggg ccgaggcgca gcgggtgaat gatgcgctga
     1141 tgcacttgct ggggtactgc cggtccggcg gcgatgccct gaccgtcctg ggcacggtgg
     1201 ccgcctcggc ccggcgggca ggacgcttgc tcctgcaccc accggccggg cgcaggagcg
     1261 ccggagcggc cgaagacacg gccgtccgtt gggcgcgcga ctggtacttg gcgcacttcc
     1321 cggcggaatg cgaggacatc ccgaggacca ttctgcggct cgcggcggag ctccgtcccg
     1381 gttggcagtg cgactcacac gccacccacg aggccatgcg cacgctcgga acgttccttc
     1441 gccgcgccgg tagacttcag gagaccttcc aacgggtgtt cggcacgtcg gttccggttc
     1501 aggagattct cgaccaactc gcggacctgg tggggcggga tggcgcatcc acccaggcga
     1561 ctatcgcagt cttgacgtgt cacgaggcaa agggccggga gttccacacc gtgttcctcc
     1621 cgggactcgc cgagggcgtg ttcccccaga catcgcggcc cccgcgtctg atctcccagc
     1681 gggccgcccg ggcgctgtgc gaccgggtgc cggggctctc tgggctgctc gagcgcccgg
     1741 aggaggccct ggctgaggag cgtcgtgcct tccacgtggc gcggtcatgt gccgtggagc
     1801 gactgctcct atcctgtcat ctggagcggg gtggacagat cgttacccca tcgcgcttcc
     1861 tcattgaggc cttgccatcc ggcgtgggga gggccgaatg tgcgctgtgt caccgccgag
     1921 cgccggccgc ggagcccgcc gattgcggcg cctgccctgt ggatccgtgt cctgggcgcg
     1981 aatcgcgcca caccgacggc ccgtggatcg tgccggtgga gacccgaccg gcggcgaccg
     2041 tgggcccccc ggtcatggcc ggccacgaga acctgccgct gagcgccagc gccctgaaga
     2101 cctatgcgaa gtgcccgcgc cgcttcttct tcgagcgcct gctacgcgtg cgcgacgaag
     2161 gcggcgatgc cgccacctat ggcaccatcg cccacgaggc gctgcgccgc atcagtcagt
     2221 gggcgcccga ggagcgcgag cccgcccgtg ccggggcgga ggtgcgggcg gtcatcgagg
     2281 agcatgccga ggacttctcc agcccagcac accggcggca gtgcgagcac gagctcacag
     2341 atgcggtcaa ccagtatctg aaccacacgg agagctacgc tcgatggcgc accatcgcgg
     2401 cggaggccgg agtcgaggtg gagctgaccg atgacacggg gcagccgcat gcgttcgtgg
     2461 gccgcctcga cctcatagcc gaggccgcga ctggagtgcg cgccctggtg gatttcaaga
     2521 ccgcaaccgg gctcgcgaag acaccggaca ccatccggaa gtggttctac gtccgggacg
     2581 gcgagaaacc cggcgccgaa cgcgactacc agctggccct ctacgcgctg gcgccacagg
     2641 ctcgtgaagg ggtggagcaa ctggcaatcc agccgatctg gcccgagtat gaaggcccac
     2701 cgtcgccgcg ggtgatcgag attggcccgg cgcccggcaa gggggtcctg agcctcgagg
     2761 agctggatga catcagccga cagctcgcca acctggccag agagatcaaa ggccgcgagt
     2821 ggttcgagcc gaaccccaag gcctgtcgca actgcccttg cctcgaagtc tgcgacggag
     2881 gggccgaggc cgaatgagca ccagcaagac gctcaaccca gagcagcggg ccatcgtcga
     2941 ggcgcccctg ggcgtgcccc tgaaagtcgt cgccggcgcc
//
LOCUS       DAOSPL010000460         2973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_33247_length_2973_cov_3.073681, whole genome shotgun sequence.
ACCESSION   DAOSPL010000460 DAOSPL010000000
VERSION     DAOSPL010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33247_length_2973_cov_3.073681"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PLQ54_09285"
     CDS             complement(<1..447)
                     /locus_tag="PLQ54_09285"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOS93492.1"
                     /translation="MTETIHSKLASLPAKPGAYVMRDADGHVLYVGKATNLRQRVRSY
                     FQPSGPKGRGPWIHEMLGHVADIDALVTHTPLEALMLELNLIRQHRPPYNTRLSDDKH
                     YPYLVITFADEYPRVEITRRAKQDGNSYYGPYANARALRRTLRTLRQ"
     gene            complement(560..1705)
                     /locus_tag="PLQ54_09290"
     CDS             complement(560..1705)
                     /locus_tag="PLQ54_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93493.1"
                     /translation="MFRVVWLLLAVVPVPCAADLVISGNDGKLDLTTGGQLVLPNPQP
                     DSISLLDFATFPPQVTHVPGVSNSVLGPPTNVAITPDETLALVASSARLVAGHPPRQV
                     LDHTLRVVDLTVRPPKVVDCIRVGLQPSGLSVSRDGRTALVANRGDGTVSVLSIRGRL
                     VRQRQVLRVCEPKQEPCHVAMADDGTRAVVSIRNRHALRVLRCRDGVWELEPGEVPVY
                     LNPYRCAICPGGRTALVAGGTTGLNADAISVVDLAVTPPRTVDHVAVGSGIESFALSP
                     DGSLVAAVAMNGSNLPDGAPGRTDNGLLVLLARQGDSLRRVQRMALGRVPEGVTFTPD
                     GRYLLVQEYVDRRIVVLPVLGARVGEPVLHIEVPGMPASIRGACGID"
     gene            1962..>2973
                     /locus_tag="PLQ54_09295"
     CDS             1962..>2973
                     /locus_tag="PLQ54_09295"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS93494.1"
                     /translation="MRLLMISPSWLPTTWGGGRTLTPPMALPVLASLTPSDVQIHLVD
                     EHVEEVDLREEADLVAMSFMTAAAPRAYEIAAHFRDRHIPVVMGGMHPTALPEEAGRH
                     CDAVVVGEAEPQWPGVLADFAAGRLQQLYRTDVRPDLAGAPIPRRDLLQQRKYLVPNL
                     VQTARGCPYACTFCSVSPVFGRRYRFRPLDEVVDEVRGLSSRSVGFSDDNIVANPERA
                     KQLFEALVPMKLQWVGQGDLSMAKDPEMLTLMRRSGCMAMFVGIESISQESLIASRKR
                     PNVGVDIEGAIRVIHRHGIDLIGSFVFGLDPDTVESCRATVDFAVKMRLAAAQFAVLT
                     PFP"
BASE COUNT          456 a         1008 c         1001 g          508 t
ORIGIN      
        1 ctgccggagg gtcctgaggg tccggcgcaa agctctggcg ttggcgtagg gtccgtagta
       61 ggagttacca tcctgcttgg cccggcgggt gatctcaacc cgcgggtact cgtcggcgaa
      121 cgtgatgact agatagggat aatgcttgtc atcggagagc cgcgtgttgt aaggcgggcg
      181 atgctggcgg atcaggttga gctcgagcat cagcgcctcg agcggcgtgt gggtcaccag
      241 agcgtcgata tcggccacgt ggccgagcat ctcgtggatc catggccccc ggcccttggg
      301 gccgctgggc tggaagtagg agcgcacccg ctgccgcaga ttcgtggcct tgcccacgta
      361 caggacgtga ccgtcggcgt ccctcatcac gtaggcgccg ggcttcgccg gtagggaggc
      421 cagcttcgag tggatggtct cggtcaacgg cgctcaggat cctcgcggat gccgcgtgtg
      481 gactctcacg ccaacctatc gctccgggcc tgcgcgtgtc aacgagcgcc gatccgggcc
      541 ctggaatcgt ctgccggtct cagtcgatcc cgcacgcgcc gcggatcgat gccggcatcc
      601 ccggcacctc gatgtgcagg actggttcgc ccacgcgggc gccgaggact gggagcacca
      661 cgatgcggcg atccacatac tcctgcacca gcagatagcg gccgtcggga gtgaatgtca
      721 ctccctccgg gacgcgcccc agggccatcc gctgtacccg gcgcagggag tccccctgac
      781 gagcgagcag gaccagcagc ccgttgtcgg tccggcccgg cgcgccgtcg ggcaggttcg
      841 aaccgttcat cgctaccgcc gccaccagcg agccgtcggg gctgagggcg aaggactcga
      901 tcccgctgcc caccgcaaca tggtccaccg tccggggcgg cgttaccgcc aggtcgacca
      961 cgctgatggc atcggcgttg agccccgtgg tgcccccggc cacaagcgcg gtgcgtcctc
     1021 cggggcagat ggcacagcgg taggggttga ggtacacggg cacttcaccc ggctcgagct
     1081 cccatacgcc gtcccgacac ctcaggaccc tcagggcgtg gcggttccgt atgctgacca
     1141 ctgcccgcgt tccgtcgtcc gccatcgcga cgtggcaggg ctcctgcttg ggctcgcaga
     1201 ctcgcagcac ctggcgttgc cggaccagcc ggccccggat cgagagcacc gatacggtac
     1261 catcgccccg gttcgccacc agggcggttc ggccatcgcg gctgaccgat agccccgatg
     1321 gctgcagacc gacgcggatg cagtccacca ccttcggcgg gcgcaccgtc aggtcgacca
     1381 cccgcaaggt gtggtcgagc acctggcgcg gtgggtggcc agccaccagt cgcgccgagg
     1441 acgccaccag cgccagagtc tcgtccgggg tgatggcgac attggtgggc gggccgagaa
     1501 cgctgttgct gacaccaggc acgtgagtca cctggggcgg gaaggtggcg aagtccagca
     1561 gtgagatgct gtcgggctgg ggatttggca gcaccagctg gccgcctgtg gtcaggtcga
     1621 gtttgccgtc gttgccggag atgaccagat cggccgcgca aggcaccgga accaccgcaa
     1681 gcagcagcca gacgacacgg aacatgagca gaccctcccc ggctcggcta cgctggaggg
     1741 cttcgtgccc ccggcgacgc cgacctgcgc catcgctcgc cggtgccggc tggccggtgt
     1801 catacccaaa agatgtgttg gtgccctcgg cggcgaatac tgcccatcgg cgggctcatg
     1861 tgggcctgct ggggaacctt gcccctttgg tctgcgattc gactgggtct gcacgccatc
     1921 ggccgcacgg ctctcaatgc cggattttct ggaggcctga cgtgcgccta ctcatgatct
     1981 ctccgtcgtg gctgcccacg acatggggcg gcggacggac tctcaccccg cccatggccc
     2041 tgcccgtcct cgcgtcactg acgccgtccg atgttcagat tcatctggtc gatgagcacg
     2101 tcgaggaggt ggatctgcgc gaggaggccg acctcgtcgc catgagcttc atgacggccg
     2161 cggctccgag agcctacgag attgcggctc acttccggga ccgtcatatc ccggtcgtca
     2221 tgggtgggat gcacccgaca gccctgcccg aggaggccgg ccgccactgc gacgctgtcg
     2281 tggtgggcga ggccgaaccg cagtggcccg gcgtcctggc cgacttcgcg gcaggccgcc
     2341 tgcagcaatt gtaccggacc gacgtgcgcc ctgacctggc cggtgccccc attccccggc
     2401 gcgacctgct ccagcagcgc aagtacctcg tacccaatct ggtccagacg gctcgtgggt
     2461 gcccctatgc ttgcaccttc tgctcggtga gcccggtctt cggccggcgg tatcgtttcc
     2521 gcccgttgga cgaggtcgtt gacgaagtcc gcgggctcag ctcccgctcc gtcgggttct
     2581 cagacgacaa catcgtggcg aaccccgaac gggcgaagca gctcttcgag gccctcgtgc
     2641 cgatgaagct ccaatgggtg ggccaaggcg atctctcgat ggccaaggac cctgagatgc
     2701 tgaccctcat gcgccgaagc ggctgcatgg ccatgttcgt ggggatcgag tccatctccc
     2761 aggagagtct catcgcgtca cgcaaacgtc cgaatgtggg tgtcgatatt gagggggcca
     2821 tccgggtcat ccaccggcac gggatcgacc tcatcggctc cttcgtgttt ggcctcgacc
     2881 ccgacacggt cgagagctgc cgggctacgg tggacttcgc ggtcaaaatg cggttggccg
     2941 cggcccagtt cgcggtgctc actccgttcc ccg
//
LOCUS       DAOSPL010000461         2969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_33316_length_2969_cov_2.707962, whole genome shotgun sequence.
ACCESSION   DAOSPL010000461 DAOSPL010000000
VERSION     DAOSPL010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33316_length_2969_cov_2.707962"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(779..1816)
                     /locus_tag="PLQ54_09300"
     CDS             complement(779..1816)
                     /locus_tag="PLQ54_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOS93495.1"
                     /translation="MIEEARGNLLQADVEALVNTVNCVGYMGRGIALQFRQAFPANYE
                     AYVKACRAGEVQPGRVLVYETDALMGPRYIINVPTKRHWRGRSRYDDVEAGLSALAQV
                     VRERGIRSVAVPPLGCGLGGLDWRRVRSMIQRAFAELPEVRVLLYTPSGTPEPQARPI
                     HTPRPTMTRSNAVVIRLMEIYGRMDYRLTLLEVQKLAYFAQEAGEPLRLRYEQGPYGP
                     YADNLNQVLLAMESHYTQGYTGGRKPDEEIALVEGAADGAAACLAKKRQTLQRLDRVA
                     ALVDGFETPYGMELLSSVHWVAKTPLKEQLTADDVVGRVHAWNTRKRRLLRQDHIHIA
                     WEHLRANGWIT"
     gene            complement(1829..2482)
                     /locus_tag="PLQ54_09305"
     CDS             complement(1829..2482)
                     /locus_tag="PLQ54_09305"
                     /inference="COORDINATES: protein motif:HMM:NF025840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="HOS93496.1"
                     /translation="MPTSTPANPRVYRITHIDNLDTLLRRGGLHAPNYVPPDGLPYRP
                     IHRQDIQEARMARPVKAGPGGVLLDYIPFFFGRRPPMLLQLHTGQVKGYKEGQGPLVY
                     LVSRIHRIVSNGCRFIFTDGHALARFTHWFDELADLAAVDWDAVQASHWADTPDDNDR
                     QRRKQAEFLVHEFLPWDLVDGIAVCGEGVRERVRNILAVHQDRHRPQVKVVRSWYYD"
     gene            complement(2594..>2969)
                     /locus_tag="PLQ54_09310"
     CDS             complement(2594..>2969)
                     /locus_tag="PLQ54_09310"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS93497.1"
                     /translation="VHAYDGVEDACATLLQFESGAIGMVDAYFCTPAVTNRVEINGSK
                     DTVVIDGMMGQGTGGTLRVIGTGQTIESSHANMYQGEITAFADAILTGGTPPVSGEDG
                     LHSQQLVEAVYESAATGKLVEF"
BASE COUNT          527 a          961 c          958 g          523 t
ORIGIN      
        1 gccgctggat gactcggtgg ggcactgggc cgactacgcg gcagtggcgg agcgactgta
       61 ccccaaccag aaggagatcg gtgcgaagcg caacgtgctc acgaagccct ggcgcgaatg
      121 gacgcgcctc gacctgtgga accactacca gtacgagccg aagttccggt tcagcgacta
      181 cggcgcgcga cttcgacgcg agcgggagct catcgccggt ctcatggctg aggggcgaca
      241 cgaactgccg ttcgagctcg acctgctacc gcccgtccag ggcaaggtca tcgccggcat
      301 gagtgccgac gggcccttcg ggggcctgag actcaaccac ttcccgccca aggcgacggc
      361 acgaatggag ggcggcaagc tcaccgccga gcaacacggc acacggtccg acttctactt
      421 cccgttcgtc tccgaccccg cctcatgccc gctggagcag ggggtgcggt acgagctgat
      481 cctccgctat aggattgccc gctccggggc cgccgcgccg ctggggctat cgatgggcgc
      541 gcgcaccacg gagggcacat ggcgtcggga cgttggggcc cgttacttct atggctctgc
      601 tggaacgagc ggcgagatcc gcacccagtt cgtgccaaag gacttcgacg actactacgt
      661 ctacctcagc ctcaacggcg atggggccgt cgaggtcgag aacgtgaggc tggtgaggga
      721 ggggaagtac agagacagat cggcgcgcgt gccatcgcgc caaccgagct gggttcggct
      781 aggtaatcca gccgttggcc ctcaagtgct cccacgcgat gtggatatga tcctgccgca
      841 ggagcctgcg cttgcgcgtg ttccacgcgt gaacgcggcc cacgacgtca tcggccgtga
      901 gctgctcctt cagcggggtc ttggcgaccc agtggaccga ggatagtagc tccatcccat
      961 agggagtctc gaaaccgtcg accagcgcag cgacgcgatc caggcgctga agcgtctggc
     1021 gcttcttcgc caggcacgcg gcggccccat cggctgctcc ctcgaccaaa gcgatctcct
     1081 cgtcgggctt ccgccctccg gtgtagccct gggtgtaatg gctttccatg gccagaagga
     1141 cttggttcag attatcagcg tatgggccat aggggccctg ctcatagcgc aggcggagcg
     1201 gttccccggc ctcctgtgcg aagtaagcga gcttctggac ctcgagtagc gtcaggcggt
     1261 agtccatgcg gccgtagatc tccatgagac ggatcacgac ggcattgctc cgtgtcatcg
     1321 tgggccgagg cgtatgaatc ggtctggcct gtggttcggg ggtgcccgat ggtgtgtaga
     1381 gcagaacgcg tacctccggg agctcggcga atgcgcgctg tatcatcgac cggacgcgtc
     1441 tccagtcgag gccacctagg ccgcacccca ggggcggcac ggcaaccgag cggatgccgc
     1501 gctcacggac cacctgcgcc agcgcgctta gtccagcctc gacatcgtcg tagcgagacc
     1561 tcccgcgcca gtggcgctta gttggcacgt taatgatgta gcggggcccc atgagtgcgt
     1621 ctgtctcata caccaacacc cgtcctggtt ggacttcacc ggcgcggcat gccttgacgt
     1681 aggcctcgta gttcgcggga aacgcttggc ggaactgcag ggcgataccc ctgcccatgt
     1741 acccgacaca gttgacagta ttgaccaggg cttcgacgtc tgcctgcagc agattgcccc
     1801 gcgcctcctc gatcatggca gagccccctc agtcatagta ccagctacga accaccttaa
     1861 cctgaggacg gtgcctgtcc tggtgcacgg caagtatgtt ccgcacccgc tcccggaccc
     1921 cctcgccgca gacagcgatc ccgtcgacca agtcccacgg cagaaactcg tgaacaagaa
     1981 actctgcttg cttgcgccgc tgacggtcat tgtcgtcggg ggtgtcggcc caatggctgg
     2041 cctgaacggc atcccagtcg acggcagcca gatctgcaag ctcatcgaac cagtgcgtga
     2101 atctggccag ggcatgccca tcagtgaaga taaagcgaca accattcgat acgatgcggt
     2161 ggatcctgga taccaggtag accagaggcc cctgcccttc cttgtacccc ttcacctggc
     2221 cagtgtggag ctgcagcaac atcggagggc gtcgtccgaa gaagaatgga atgtagtcga
     2281 gcagtacgcc ccccggtccg gccttgaccg gccgggccat ccgggcctcc tgtatgtcct
     2341 gccggtggat cgggcggtaa ggcagaccat ccggaggcac gtagttgggg gcgtgtagcc
     2401 cacctcggcg caggagcgtg tccaggttgt cgatgtgcgt gatgcggtag acccgtgggt
     2461 tagcaggagt actcgtcggc attgcgagcc caccgttgcg gagcaaatgc agccagatga
     2521 cagtgttcca gtgctgggca gcgcagaacg agtctcgcgg agcccgcggt ccttcggcca
     2581 tccgtccgac gccctagaac tccaccagct tcccggtcgc cgcggactcg tagacggcct
     2641 cgacgagctg ctggctgtgg aggccgtcct cgccggagac cggcggcgtg ccgcccgtca
     2701 ggatggcgtc ggcgaaggcc gtgatctcgc cttggtacat gttcgcatgc gaggactcga
     2761 tggtctggcc ggtgccgatg accctgagcg tcccacccgt gccctggccc atcatgccgt
     2821 cgatgaccac cgtgtccttg ctgccgttga tctcgacgcg gttggtgacg gccggcgtgc
     2881 agaaataggc atcgaccatg ccgatggcac cactctcgaa ctgcagcagc gtcgcgcagg
     2941 catcctcgac gccatcgtag gcgtggacg
//
LOCUS       DAOSPL010000462         2967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_33347_length_2967_cov_2.854739, whole genome shotgun sequence.
ACCESSION   DAOSPL010000462 DAOSPL010000000
VERSION     DAOSPL010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33347_length_2967_cov_2.854739"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..747)
                     /locus_tag="PLQ54_09315"
     CDS             complement(172..747)
                     /locus_tag="PLQ54_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002618870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOS93498.1"
                     /translation="MQAAGAIRRICVYCGANEGSEASFVRTARELGRSMAAHDLELVY
                     GGGSVGMMGALADAVLESGGRVIGVIPEALARRELAHPGASEMHVVPDICCRRAKMTE
                     LADAFIALPGGFGTFEELTGTLTAVQLGLLDKPVGVLNVDGYFDHLLALVEHAAARGF
                     IAAEDRDALMVAGSPGELLRQMGALGDGHTL"
     gene            905..2623
                     /locus_tag="PLQ54_09320"
     CDS             905..2623
                     /locus_tag="PLQ54_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOS93499.1"
                     /translation="MGAAGHHDAEAALRESEGLLRATLESTADGILVVGADGRVIHAN
                     GRFAQMWGVPEPLLRGGDDEKLLQHVLSQLGEPEEFLARVQALYQSDEEGLETIHFRD
                     GRVFERFTRPLMREGRVAGRVWSFRDITARHRAEERLRLDEARLEALVRLSEMADAPL
                     RAITDFAMESGVKLTRSTMGYLAFTNEDESVLTMHSWSKTAMAECAIEDKPVVYPVET
                     TGLWGEAVRQRRPIITNDYGAPNPAKRGYPEGHVRVTRHLNIPVFDGDRIVAVAGVGN
                     KSTDYDETDVIQLRLLMDGMWRLIHRRRAEEALRQARDDLERRVEERTRELARSNAEL
                     ERFASVASHDLQEPLRKVVAFGDRLAEACGSALDESGRDYLDRMQNAARRMQGLISDL
                     LAYSRVISRAQPFGPVNLADVAREVVEDLETRIEMAGGRVEVGPLPVIDADRTQMRQL
                     LQNLIANGLKFHRPDLAPVVRVWGELADGVVLSPQRGPERVCRIEVSDNGIGFDPKYA
                     DRIFGVFQRLHGQSEYEGTGIGLAICRRIAERHGGQIEVESAPECGARFTVTIPACQP
                     EEGDTP"
     gene            2620..>2967
                     /locus_tag="PLQ54_09325"
     CDS             2620..>2967
                     /locus_tag="PLQ54_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014261377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS93500.1"
                     /translation="MNRSAEAITILVADDDREDCQLLEDAFRESRIANRLVFVHHGED
                     LTDYLWRRGRFADPDSAPRPGLILLDLNMPRKDGREALAEIKADPSLRQIPVVVLTTS
                     KAEEDIYRSYDLGV"
BASE COUNT          494 a         1028 c          984 g          461 t
ORIGIN      
        1 ctcgaccgca ccggatcccg tccacgaagc gcagctcaac tcctccgcga tgcccgccgt
       61 tcaacgcagc gacccatctc cgggagcgtc agcaccacca cggcgtcccg gcccacccca
      121 tggcttccgc ccagcggagg gccagccctt gaggagatcc ccacgaggac tctagagtgt
      181 atgcccatcg ccgagcgccc ccatctgtct gagcaactcc ccgggcgatc cggcaaccat
      241 gagcgcgtcg cggtcttctg ccgcgatgaa ccctcgggcc gccgcatgct cgaccagcgc
      301 caggagatgg tcgaagtagc cgtcgacatt cagcactccc acgggcttat cgagcagccc
      361 gagctgcacg gccgtgaggg ttccggtcag ctcctcaaag gttccgaacc caccgggcag
      421 cgcaatgaaa gcgtcagcca gctcggtcat cttggcccgc cggcagcaga tgtcgggcac
      481 cacgtgcatc tccgacgccc ccgggtgggc gagctcgcga cgcgccaagg cttccgggat
      541 gacaccgatt acgcgcccgc ctgattcgag cacggcgtcc gccagagcgc ccatcatgcc
      601 cacactaccc ccaccgtaca ccagttcgag gtcatgggca gccatggacc gtcccagctc
      661 gcgagccgtc cggacgaagg acgcctcgct tccctcattc gcgccgcagt acacgcagat
      721 tcgtctgatt gcacccgctg cttgcacgtc ggtcctcccg gggccccacc cgctacggcc
      781 acgcctccta tcatagcgcc cggcggcgtc gttcgtgcgc cgggaggagg accgcgcccc
      841 catcgggaac cgtagacgtg cagacggacc atcgcgcggg cgcggctgag tggaggcggt
      901 ggtgttgggc gccgcaggcc accacgatgc tgaagcggct ctccgggaga gcgaggggct
      961 gcttcgtgcc accctcgagt cgaccgccga cgggatcctc gtcgtcggtg ccgatggtcg
     1021 cgtgatccac gccaacggcc gcttcgccca gatgtggggc gtccccgagc ctctcctgcg
     1081 gggcggcgac gatgagaagc tgctccagca cgttctgagc cagcttggcg agcccgagga
     1141 gtttctcgcc cgggtccagg cgctctacca gtccgatgag gaaggccttg agaccatcca
     1201 tttccgcgac ggccgggtgt tcgaacgctt cacccgtcca ctgatgcgcg aggggcgagt
     1261 cgcgggccgc gtgtggagct tccgcgacat caccgcacgc caccgggccg aggagcggct
     1321 ccggctcgac gaggcccgcc tcgaggctct ggtccggctc agcgagatgg ccgacgcgcc
     1381 cctgcgcgcg atcaccgact tcgccatgga gtcgggcgtg aagctgaccc gcagcaccat
     1441 gggctacctc gcattcacga acgaggacga gtccgtcctc accatgcact cgtggtcgaa
     1501 gacggccatg gctgagtgtg ccatcgagga caaacccgtg gtctacccgg tcgagacgac
     1561 gggcctgtgg ggcgaggccg ttcggcagcg gcgccccatc atcaccaatg attatggggc
     1621 acccaacccg gccaagaggg ggtaccccga gggccacgtg cgcgtcaccc gccacctgaa
     1681 catcccggtg ttcgatggcg accgcatcgt ggctgtggcg ggcgtcggca acaagagcac
     1741 cgattacgat gagaccgatg tgatccagct ccggctgctc atggacggca tgtggcggct
     1801 gatccatcgg cggcgggccg aggaggctct ccggcaggcg cgcgatgacc tcgaacgccg
     1861 ggtggaggag cggaccagag agctggcgcg gtccaacgcc gagctcgagc gcttcgcatc
     1921 cgtggcctcg cacgacctgc aggagccgct gcgcaaagtg gtggcctttg gcgaccgcct
     1981 ggccgaggcc tgcggttctg ccctcgacga gagcggccgc gactatctgg atcgaatgca
     2041 gaatgccgcc cggcggatgc aggggctcat ctctgacctc ctggcttact ccagggtgat
     2101 cagccgggcc cagccctttg ggccggtgaa cctggccgac gtggcgcggg aagttgtgga
     2161 ggacctcgag acgcggatcg agatggcggg aggacgggtc gaggtggggc cgctgcccgt
     2221 catcgatgcc gatcggactc agatgcgtca gctccttcag aatctcatcg cgaatggcct
     2281 caagttccat cggcccgacc tggccccggt ggtccgcgtc tggggcgagc tcgccgacgg
     2341 cgtcgtgctc agtccacaac gaggtccgga gcgcgtgtgc cgtatcgagg tctcggacaa
     2401 cggcattggg ttcgacccca agtatgccga ccgcatcttc ggtgtcttcc agcgcctcca
     2461 tggccagagc gagtatgagg gcacgggcat cggcctcgcc atctgccggc gcatcgccga
     2521 acggcacgga ggccagatcg aggttgagag cgcgccggag tgtggggcca ggttcaccgt
     2581 cacgatcccg gcgtgccagc cggaggaggg agacacgcca tgaaccggtc ggccgaggcg
     2641 atcacgatcc tggttgccga tgatgaccgg gaggactgcc agcttctcga ggatgccttc
     2701 cgcgagagcc gcatcgccaa tcgcctggtc ttcgtacacc acggcgagga tctgacggac
     2761 tacctgtggc gccgggggcg tttcgctgac cctgattcgg cgccccgtcc ggggctcatc
     2821 ctccttgatc tgaacatgcc gcgcaaggac gggcgcgagg ccctggcgga gatcaaggcg
     2881 gatccatcgc tccgacagat ccccgtggtt gtgctcacca cgtcaaaggc cgaggaggac
     2941 atctaccgct cctacgacct gggcgtg
//
LOCUS       DAOSPL010000463         2957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_33491_length_2957_cov_2.493108, whole genome shotgun sequence.
ACCESSION   DAOSPL010000463 DAOSPL010000000
VERSION     DAOSPL010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33491_length_2957_cov_2.493108"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PLQ54_09330"
     CDS             <1..709
                     /locus_tag="PLQ54_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOS93501.1"
                     /translation="PHDFERVQDESRLAITVIDPERGVHTEVNELGPHVTSNELADFI
                     ERYSCLLADVCLAAFAGSAPPGVGPSIYGQLIRMAKARGVATFLDARGEWLRQGVLAG
                     PDMVKPNQAELGHLLGRVPSGIREMALAAAELCARGVRQVVVTLGADGALGVSEGVAL
                     HGWLEPVPVESAVGSGDAFAAALSAGWVRGAPLEESLRRGIACGTASAMRLGPAFVYR
                     DEVDCLADRVRMEPVPI"
     gene            767..1117
                     /locus_tag="PLQ54_09335"
     CDS             767..1117
                     /locus_tag="PLQ54_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOS93502.1"
                     /translation="MLHFLGFFGPIGTQELIIIVLLVLLLFGAKKVPEFMRSMGEGVR
                     EFRKSSREIMREFEEEVSRPEPARPIPPPAPVQAAPTPAAASSETEGRDTRIESSDNQ
                     DQTVGTGSAGHSEY"
     gene            1136..1684
                     /locus_tag="PLQ54_09340"
     CDS             1136..1684
                     /locus_tag="PLQ54_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93503.1"
                     /translation="MRTLSRAIGSHRAIAALVVIGLCSAAAYAAITIGKVLPPSDAVA
                     GWKIVPKTYKECPNPSGLAELYDGGWEKYRDAGIIAAATQTYKGSAKQIVIYAHEFSS
                     AERAKAYYASAQKNAKGKITSVQVAGGQAFYSVTGPATVGNVVRGKVQCQVAAMGSTA
                     DMQQAVAAFLKVLANRVGQVYK"
     gene            complement(1760..>2957)
                     /locus_tag="PLQ54_09345"
     CDS             complement(1760..>2957)
                     /locus_tag="PLQ54_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOS93504.1"
                     /translation="AADGSDRREFLLGYFDRPGDLATATFLKGYQWPFHAERTQGCGS
                     SLVDVAVWMEEQSGRHVFVDFGRNAAALELLAPEAREYLIRSGAYQPTPLERLAHMNP
                     AAVAVYEEHGVDLREPLEVTVCAQHCNGGLRGDIWWETSVPHLFAIGEVNGSHGVRPG
                     GSALNAGQVGGLRVAQRIGRVYRATPLPPHVFEPTVMPQLRAALDRIRWLMAAGEGAP
                     SPGRVRREIQARMTAHGAFLRSASGLADAVNGARLLLHRIRAEGQRLASVEQLATAVE
                     NEHLCLTHLALLQTMKAYVDAGGGSRGSCMVLDPAGDRTVRTPRGQQLRHRSENLTMR
                     AEILETRLKAPAGVDFEVVAIPTRPLPDDTSWYETTWREFEAGRVFEDEPQVKTFLVP
                     KGASPL"
BASE COUNT          508 a         1008 c          989 g          452 t
ORIGIN      
        1 cccgcacgac ttcgagcgcg tccaggacga gagccgactc gccatcaccg tcatcgatcc
       61 cgagcgtggc gtgcacaccg aggtgaacga gctggggcct catgtaacct cgaacgaact
      121 cgccgacttc atcgagcggt acagttgcct gctcgcagac gtgtgtctgg cggccttcgc
      181 ggggagcgct ccgcccggtg tcggtccatc gatctacggc caactcatcc ggatggccaa
      241 ggcccgagga gtcgcgacct ttctcgacgc ccgcggcgag tggctccgcc agggcgtcct
      301 cgccgggccg gacatggtca aacccaacca ggcagagctc ggtcacctgc tgggccgggt
      361 tccctctggc atccgggaga tggctctcgc ggcggccgaa ctgtgcgcgc ggggtgtccg
      421 ccaggtggtc gttacgctgg gcgccgacgg agccttgggt gtctccgaag gcgtcgccct
      481 ccatgggtgg ctggaaccgg tacccgtgga gtcggccgta ggtagcggcg atgccttcgc
      541 ggcggcgctg agcgccggct gggtgcgagg cgcgcccctc gaggagtcgc tccggcgagg
      601 catcgcctgt ggcacggcca gcgccatgag actcgggccc gccttcgtct accgcgacga
      661 agtggattgc ctggccgacc gtgttagaat ggagccggtg cccatctgag tgtgtggcgc
      721 gtcctggggc gaatcctagg aagacccggg agaagaggag acggccatgc tgcacttctt
      781 ggggttcttc ggtcccattg ggacccagga actgatcatc atcgtcctat tggtgttgct
      841 gctcttcggg gccaagaagg ttcccgagtt catgaggtcc atgggcgagg gtgttcgcga
      901 gttccgcaag tcctcgcggg agatcatgcg cgagttcgaa gaggaagtct cgaggcctga
      961 acccgcccgg ccgatcccgc caccggcgcc cgtacaggcc gccccgacgc cggccgccgc
     1021 cagttcggag accgagggac gtgacacgcg catcgagagt agcgacaacc aggaccaaac
     1081 agttgggacc ggctccgccg ggcacagcga atactagggg taggtgagcc ccaccatgcg
     1141 tacactcagt cgggcgatcg ggtcgcatcg cgcgatcgcg gctctggtgg ttatcggact
     1201 ctgtagtgcc gcggcctacg cggccatcac gatcggcaag gtcctgccgc ccagcgacgc
     1261 cgtggcgggc tggaagattg tgcccaagac ctacaaggag tgccccaacc cgagcggact
     1321 ggccgagctc tatgacggag gctgggagaa gtaccgcgat gcgggcatca tcgccgccgc
     1381 cacgcagacc tacaagggct ccgccaagca gatcgtgatc tatgcccacg agttctcgag
     1441 tgccgagagg gccaaggcct actacgcctc ggcgcagaag aatgccaagg gcaagatcac
     1501 cagcgtgcag gtcgccggcg gccaggcgtt ctactcggtg acgggcccgg ccaccgtggg
     1561 taatgtggtg cgcggcaagg tgcagtgcca ggtcgccgcc atgggtagca cggccgacat
     1621 gcagcaggcg gtcgctgcgt tcctgaaggt gctcgcgaac cgcgtgggcc aggtctacaa
     1681 gtaagtctcc gatcgccaag ccgagtacgg cctctcggcc gggccccgca cccggcaatg
     1741 ctggcgtccg gaagggtact cacagaggcg aggcgccctt cggtacaagg aacgtcttaa
     1801 cctgcggctc gtcctcgaac acccgccccg cctcgaactc ccgccatgtg gtctcatacc
     1861 acgacgtgtc gtccggcagg ggacgggtcg ggatcgcgac cacctcgaaa tccactccgg
     1921 ccggggcctt cagccgcgtc tcgaggatct ctgcacgcat cgtgaggttc tccgaccgat
     1981 ggcgaagctg ctggccccgg ggcgtgcgaa cggtgcggtc acccgcggga tccagaacca
     2041 tgcacgaacc ccggctaccg ccaccggcgt cgacataggc cttcatcgtc tgaagcagcg
     2101 ccaggtgggt gaggcacagg tgctcgttct cgacggcagt ggcaagctgc tcgacggagg
     2161 ccagccgctg cccctcagcc cggatccggt gcagcaggag ccgggcgcca ttcacggcat
     2221 cagcgagtcc ggaggccgac cgtaggaagg cgccgtgcgc cgtcatccgg gcctggatct
     2281 ctcgccgcac ccggcccggc gagggagcgc cctcgccggc cgccatgagc caacggatcc
     2341 ggtcgagcgc cgccctcaac tgcggcatca ccgtcggctc gaacacgtgt ggcgggaggg
     2401 gcgtcgcgcg gtacacacga ccaatccgct gggccacacg caggcccccc acctggccgg
     2461 cgttgagcgc gctgccgccg ggccgtaccc catgggagcc attgacctcg ccgatggcaa
     2521 acaggtgggg caccgaagtc tcccaccaga tgtcgcccct caggcctcca ttgcagtgct
     2581 gggcacacac ggtcacctcg aggggctcgc gcaggtccac gccgtgctcc tcatagactg
     2641 ccaccgcggc cgggttcatg tgagcaagcc gctcgagtgg cgtgggctga taggccccgc
     2701 tccgaatcag atactcccgc gcctccggcg ccagaagctc gagagccgcc gcattgcgcc
     2761 cgaagtccac aaacacgtgt cgccccgact gctcttccat ccagacagcc acgtcaacca
     2821 gtgaggagcc acacccctgc gtccgttcgg catggaatgg ccactgataa cccttaagga
     2881 acgtggccgt cgccagatca cccgggcggt cgaagtagcc caaaaggaac tcgcggcggt
     2941 cgctgccatc ggcagcc
//
LOCUS       DAOSPL010000464         2955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_33519_length_2955_cov_2.680690, whole genome shotgun sequence.
ACCESSION   DAOSPL010000464 DAOSPL010000000
VERSION     DAOSPL010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33519_length_2955_cov_2.680690"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="PLQ54_09350"
     CDS             <1..1211
                     /locus_tag="PLQ54_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93505.1"
                     /translation="VGAPGTDAAAAAQLRDDGSYHDGGYWATPLPWFMVALMRRDEVK
                     AARTFCDAVADFRSTGDINEWINDHGRGVPEYCASATMPLEGVRLLRAHLEAAGKSLP
                     ADLDRQLAEDAAWLEGRAREIVRRGSRLADNGVRMFTPDATGQYGQFWVRDWYYMIEG
                     LPDAFAREEVRDGYLFLAAAQRADGCMPDRVRPDGKGVYSPGGEETPLSANGSTDQSP
                     MMVLLCHKYWELHGDLEPFRATAGALDRAMRFTPRNTENGLVTITDSRLFRPWSFQDM
                     IPLEGDDLFSSVLFWDACGKLAELYSALGDGAAAAEWRAEGARVRAALGSLWDAAAGA
                     FVGASRHWPQPYVWGSAYAVYAGAANDDQAARVAEFLDVHYDLIVKRGQIRPLLKGTF
                     WGRPEPEYVP"
     gene            complement(1367..2128)
                     /locus_tag="PLQ54_09355"
     CDS             complement(1367..2128)
                     /locus_tag="PLQ54_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93506.1"
                     /translation="MTQRLPIPLALAATLLMTLSALAQATIDATRIETPPTIDGIMHE
                     GEWADVPRVETKTALFPLSQIEAALQGVAEGEPLVCEAYFANDSENLYIALVIKDVTF
                     NGTVDMAMGLVDTDACWIDFDSDGIPGPSIGDDRKAVMPTATGLYLDQHRVKETGDED
                     HKDDAQVDGEARMMHSTGQGKGDYTAEFRIPLNSGDADDLAAEPGDVIGLNSIFIDGF
                     SSAGPANVRLAILIGPKLADMAMGGSQFVKLALAK"
     gene            2307..>2955
                     /locus_tag="PLQ54_09360"
     CDS             2307..>2955
                     /locus_tag="PLQ54_09360"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOS93507.1"
                     /translation="MDATSLVGRLADIGIALTSIRSLDRLLEHIVVQACDFASCDGGT
                     VYIREGECLRLAVWRNAALARRSGRQAEVVREGRLLPISSRWIAGYVASEGQVLNLPD
                     VYCLPPGSPFAFDPNFDRENDYHTRSMLCVPMNDATGDNVGALQLVNAMDRAGRVVAF
                     PAAIEPLMRSLASQAAVAIRNAQLDEKLKAAYFDTILRLSVAAEYRDTDTSRHVRR"
BASE COUNT          445 a          916 c         1042 g          552 t
ORIGIN      
        1 cggttggcgc cccgggtacc gacgcggccg ccgcggccca gcttcgcgat gacggctcgt
       61 accacgatgg tggctactgg gccacgccct tgccctggtt catggtggcg ctcatgcgcc
      121 gcgatgaggt caaggctgcg cggaccttct gtgacgcggt ggcggatttc cgctccaccg
      181 gcgacatcaa tgagtggatc aacgaccatg gccggggagt ccccgagtac tgtgcgagtg
      241 ccacgatgcc gctcgaggga gtgcggctcc ttagggctca cctggaggca gccggcaagt
      301 cgctgccggc cgatctcgac cggcagctcg cggaggatgc cgcgtggctt gagggccgtg
      361 cgagagagat cgtccgacgc ggcagccgtc tcgcggacaa cggtgttcgc atgttcacac
      421 cggacgccac gggccagtat ggtcagttct gggtccgcga ctggtactac atgatcgagg
      481 ggctgcccga tgcgtttgca cgcgaggagg tccgggacgg ttacctcttc ctggccgcgg
      541 cccaacgcgc ggatggctgc atgccggacc gcgtccggcc cgacggcaag ggggtttact
      601 cccctggcgg cgaggagacc cctctgtcgg ctaacggctc gacggaccag tcgccgatga
      661 tggtacttct ctgccacaag tactgggagt tgcacggtga tctggagccc ttccgggcta
      721 cggccggcgc gctcgaccgc gcgatgcgtt tcaccccgcg taacacggag aacggtctcg
      781 tcacgatcac cgactcgagg ctgttccggc cctggagctt tcaggacatg atcccgctcg
      841 agggcgatga tctgttcagc tcggtgctgt tctgggatgc gtgcggcaag ctggccgagc
      901 tgtattcggc tctgggtgat ggggccgcgg ccgcggagtg gcgtgccgag ggcgcaaggg
      961 tcagggcggc gcttggcagt ctatgggacg ccgctgccgg ggccttcgtc ggggcgagcc
     1021 gtcattggcc ccagccctac gtgtggggct cggcttacgc cgtctacgcg ggcgcggcca
     1081 acgatgacca ggcggcgcgt gtcgccgagt tcctggatgt ccactacgac ctgatcgtga
     1141 agcggggcca gatacggcct ctactcaagg gcacattctg gggcaggccc gagcccgagt
     1201 acgtgccgtg agagggtggc cctgagccta cagcgccgcc ctggtggaga ggcgcgtctt
     1261 gccccgcgac ggtctatgga ccccagttcg ggttgcccac cgacgctccg ggctgcccga
     1321 tcggcttccc cgcgcgagca gctcggagcg ttgtctgtga gtacggtcac ttggcgagcg
     1381 cgagcttgac gaactgcgag ccccccatgg ccatgtcggc cagcttgggg ccgatcagga
     1441 tggcgagacg gacattcgcc ggacccgccg agctgaaccc gtcgatgaat atggagttga
     1501 ggccgatcac gtcgcccggc tccgccgcca ggtcgtcggc atcgcccgag ttgaggggta
     1561 tgcggaactc ggcggtatag tcgcccttcc cctgcccggt cgagtgcatc atgcgggcct
     1621 cgccgtcgac ctgagcgtcg tccttgtgat cctcatcgcc ggtctccttg acgcggtgct
     1681 ggtcgaggta gagacctgtg gcggttggca tcacggcctt gcggtcatcg ccgattgacg
     1741 gaccggggat gccatccgag tcgaagtcga tccagcacgc atcggtgtcg actagaccca
     1801 tggccatgtc gacggtgccg ttgaaggtca cgtccttgat gacgagcgcg atataaaggt
     1861 tctccgagtc gttggcgaag taagcttcac acacgagtgg ctcgccttcg gccaccccct
     1921 gaagcgcagc ctcgatctgg gacagcggga acagggcggt cttggtctcg actctgggga
     1981 catcggccca ctcaccctcg tgcatgattc catcgatggt gggtggagtc tcgattcgcg
     2041 tggcgtcgat ggtggcttgc gcgagtgcgc tcaaggtcat cagtagtgtc gcggcgaggg
     2101 caagtgggat gggcagtctc tgtgtcatcc gtgggtccct tctggcgccg acggtggcgc
     2161 cccaggtgtg tgagctacgg agagaggacg gtggcgcgcc agtccggttt cagtctagcg
     2221 ccacgccgca tcctgacgca gccggaggac gcaaggggga catccgggaa tgcccatggg
     2281 tgtcggcttc cggaggaggg gtcgtcttgg acgcgaccag ccttgtgggg cggttggccg
     2341 atattggcat tgccttgacc agcattcgca gcctggaccg cctcctggag cacatcgtcg
     2401 tgcaggcatg cgacttcgct tcttgtgatg gtgggactgt ctacatccgc gagggcgagt
     2461 gcctgcggct ggctgtgtgg cggaatgcgg cactggctcg ccggtcgggg cgccaggccg
     2521 aggtggtccg cgagggacgg ctgctgccca tctcgagccg gtggatcgcc ggctatgtcg
     2581 cgtctgaggg acaggtgctc aacctgcccg atgtctattg cctgccgcct ggtagtcctt
     2641 tcgcgttcga ccccaacttc gatagggaga acgactacca cacccggtcc atgctgtgcg
     2701 tgccgatgaa cgacgcgacc ggggacaatg tgggcgcgct gcagttggtc aacgcgatgg
     2761 accgtgcagg tcgcgtggtg gcgttcccgg ccgccatcga gcccctcatg cggtcactgg
     2821 catcgcaggc cgcggtggcc atccgcaacg cacagctcga tgagaagctg aaggcggctt
     2881 actttgacac gatcctgcgg ctctcggtag ctgccgagta ccgtgatacc gacacgtcgc
     2941 gtcacgtcag gcggg
//
LOCUS       DAOSPL010000465         2952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_33556_length_2952_cov_2.610287, whole genome shotgun sequence.
ACCESSION   DAOSPL010000465 DAOSPL010000000
VERSION     DAOSPL010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33556_length_2952_cov_2.610287"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="PLQ54_09365"
     CDS             complement(<1..1153)
                     /locus_tag="PLQ54_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93508.1"
                     /translation="MPSRGLAVAGHALPKWGVAAACVGFALMGAAGRAASQPASSASA
                     LTAQARAALDQRLLNEAVDLARQALEADRTLAAAWKVMGDARARRGHRSRQDKERAIE
                     AYAQYQLLSHDASVQGAIERLYLEGDLPRWLAKDTLPVLPVHPKADTVTVRRSIKPGD
                     ADLRIPVCVTTEMMYPPGTTLEHPRYGAGFNRVVCGYITDANDPEGRLYERARVYFTS
                     LLTSKSKVDLQPIAVNTLLLVLRLMCYQAAYLAPDGLSAPDNQAPLELWLVEDREPGG
                     EQRRGEMAVFGAGAARSPLEWIRLVCHEFGHYALPPIGVYTEPEPWANGLLGEYLFPV
                     WLWRNQGGGTVAWGDDAVDLTPFLNSRCGTLLQAFFAQMPDGNALYKRDA"
     gene            complement(1135..2103)
                     /locus_tag="PLQ54_09370"
     CDS             complement(1135..2103)
                     /locus_tag="PLQ54_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93509.1"
                     /translation="MAEIGWQGIRITVPDDWEPGEFSGDGQSGHMRIDSMMQPRALVR
                     WLAPSATKDVFGKVATPIQGVDRILDNYIKALTKGERKQKKHIEADTSTRLLSKRKMG
                     QRTVRTYSWRCPSDGRLAHGMVWHCKECGRTVIAEVNGHEHEDVAGWAETILPTVTDH
                     AMDGQVLWAFFDLRVLVPEEFRLENSVMVGGKVELRLENGKREVISAQRWVANVALRG
                     GDLEAWAKRDLFKPLRQQHTFKYTADSWFGHPGIRVDGRRKGPKEHLGFMWGRITGRE
                     RPFFLDGRIWHCEPTNKIHSLHTLTRESNRELIDRIQETMRCHPED"
     gene            complement(2157..>2952)
                     /locus_tag="PLQ54_09375"
     CDS             complement(2157..>2952)
                     /locus_tag="PLQ54_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOS93510.1"
                     /translation="ISIQPSEFSKLALIITLAAYMADRGAEMRNAGPFLLSFAEVVLP
                     PVALIVAQRDLGTSLVCIAIWLTVMYTAGCRWNHLALFVLGGLVLFGMLWKYHVMPAE
                     QLSRITGWLMPYRDPTGDGYHYITTQATIGAGGWLGLGFRHGPMTQNQIVPHQDTDMV
                     FSVLGEEWGLAGCTVVLVLYGFLVWRGFSTVAEAKNDLGRYVAAGVTGMLLCHVVVNI
                     SMQIRLLPITGIPLPLLSSGGSNLTVTMAALGLLINVHMRRKKLTF"
BASE COUNT          492 a         1010 c          931 g          519 t
ORIGIN      
        1 tcgcgtccct cttgtacagc gcgttgccgt ccggcatctg ggcgaagaac gcttgcagca
       61 gcgtcccaca tcgggagttc aggaacggcg tcaggtccac cgcatcatcg ccccaggcta
      121 ccgtccctcc cccctggttg cgccagagcc acacggggaa aaggtactcg ccgagcagac
      181 cgttggccca gggctcgggt tcggtgtaga cgccgatggg cgggagcgcg tagtgtccga
      241 actcgtggca gaccagccgg atccactcca gaggactccg ggccgcgccg gcgccgaaca
      301 cggccatctc gcccctacgc tgctcgccgc cgggctccct gtcctcgacc aaccacagct
      361 ccagcggcgc ctgattgtct ggggcgctca gcccatcggg agccagatag gcggcctggt
      421 aacacatgag gcgcaggacc agcaggagcg tgttgactgc aatgggctgc aggtcgactt
      481 tgcttttcga cgtgagcagc gaagtgaagt acacgcgtgc ccgctcgtag aggcgtcctt
      541 ccgggtcatt ggcgtccgtg atgtagccgc agaccacgcg gttgaacccg gcaccgtagc
      601 ggggatgctc cagcgtagtg cccggtgggt acatcatctc ggtggtcacg cacacgggga
      661 tccgcaagtc cgcgtcgccc ggcttgatcg accggcgcac cgtcacggtg tcggctttgg
      721 ggtggaccgg caggactggg agtgtgtcct tggcaagcca tcgtggcagg tcgccctcga
      781 ggtagaggcg ttcgatcgca ccctggaccg aagcgtcgtg gctcagcagc tggtactggg
      841 cgtaggcctc gatcgcccgt tccttgtcct ggcggctacg gtgcccccgc cgggcccgcg
      901 cgtctcccat caccttccag gccgccgcga gtgtgcggtc ggcctcgagc gcctgccggg
      961 ccaggtccac cgcctcgttg agcaaacgct ggtcgagcgc tgcccgtgcc tgagcagtca
     1021 gcgcggaagc cgacgacgcg ggttggcttg cggccctgcc ggcagcaccc atgagtgcga
     1081 accccacaca ggcggccgca accccccact tcggcagggc atggccggcc acggctagtc
     1141 ctcgggatgg catcgcatcg tctcctggat cctgtcgatc agctcccggt tcgactcccg
     1201 ggtcagtgtg tgcaggctgt ggatcttgtt ggtgggttcg cagtgccaga ttcggccatc
     1261 caggaagaac gggcgctccc ggccagtgat acgcccccac atgaagccca ggtgctcctt
     1321 gggtcccttc cggcgtccat cgacccggat cccagggtgc ccgaaccacg agtcggccgt
     1381 gtacttgaac gtatgctgct gacgcaaggg cttgaacagg tctcgtttgg cccaggcctc
     1441 caggtcacca ccgcgcaggg ccacattggc cacccaccgc tgagcggaga tgacctctct
     1501 cttgccgttc tccaggcgta gttcgacctt gccccccacc atgaccgagt tctcgagtct
     1561 gaactcctcg ggcaccagca cgcgcaggtc gaagaaggcc cagagaacct ggccgtccat
     1621 ggcgtggtct gtcacggtcg ggaggatggt ctccgcccag ccggccacgt cctcgtgctc
     1681 atggccgttg acctcggcaa tgaccgtccg cccgcactcc ttacagtgcc agaccatgcc
     1741 gtgggcgagc cggccgtcgg aagggcatcg ccacgagtat gttcgcacgg tgcgttgccc
     1801 catcttgcgt ttcgatagga gccgcgtcga cgtgtccgcc tcgatgtgct tcttctgctt
     1861 gcgctctccc ttggtcaggg ccttgatgta gttgtcgagg atgcggtcga cgccctggat
     1921 cggggtggcg actttgccga acacgtcctt cgtcgccgag ggtgccagcc accgaaccag
     1981 cgctcgaggc tgcatcatcg agtcgatgcg catgtggccg gactgcccat cgcccgagaa
     2041 ctcgcccggt tcccagtcat cgggcacggt gatgcggatc ccctgccagc cgatctccgc
     2101 catatgccgc tggtttccta cccctatctg ccggcgccgg gctgccctgc ccggtactag
     2161 aaggtcagct tcttgcgcct catgtgcaca ttgatgagca gtcccagcgc cgccatggtc
     2221 accgtcaggt tcgagccacc cgagctcagt aggggcagtg ggatgccggt gatcggcagc
     2281 agccggatct gcatcgatat gttgacgacc acatggcaca ggagcatgcc cgtgacgccg
     2341 gccgccacgt atcggcccag gtcgtttttc gcctcggcca cagtcgagaa gccgcgccac
     2401 accaggaagc catagaggac gagcaccacc gtgcagccgg ccaagcccca ctcctcgccg
     2461 agcaccgaga agaccatgtc cgtgtcctgg tgcggcacga tctgattctg tgtcatgggc
     2521 ccatgacgga acccaagccc caaccatcca ccggccccga tcgttgcctg ggttgtgatg
     2581 tagtgatacc catcgcctgt ggggtcgcgg tacggcatga gccatcccgt gatgcgcgac
     2641 agctgctccg cgggcatcac gtggtacttc cagagcatgc cgaacaggac caggccacca
     2701 agcacgaaca gcgccagatg gttccagcgg caccccgcgg tgtacatcac cgttagccaa
     2761 atcgcgatgc aaaccagcga ggtccccagg tcgcgctggg ccacaatcag cgccacgggc
     2821 ggaagcacga cctcggcgaa cgacagcagg aacggccccg cattccgcat ctccgcaccc
     2881 cgatcggcca tgtaggccgc cagggtaata atgagggcga gcttgctgaa ctcggagggc
     2941 tggatgctga tc
//
LOCUS       DAOSPL010000466         2944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_33669_length_2944_cov_2.436483, whole genome shotgun sequence.
ACCESSION   DAOSPL010000466 DAOSPL010000000
VERSION     DAOSPL010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33669_length_2944_cov_2.436483"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..988)
                     /locus_tag="PLQ54_09380"
     CDS             complement(95..988)
                     /locus_tag="PLQ54_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93511.1"
                     /translation="MTRSFVRCAPLFLGVGILVALAGCTSPFFTGSGDLTNTIVPGQS
                     NEATAATVRAVLSVDVANPGVNQFYLTSLVLQPEGGVGVLRGVPATFHVLSAGTLAAA
                     QAGVFAVANQLCPETEDAIWYENKNAKFQSITNQNGVIQVAPQSAGIDPGQENTLGYD
                     RPRLPVLPGAMGWLTANAITLEQSTTFGQRKQQFLGNVRLNYASPARLTGTNANISEV
                     VFEIGVPVYNQLASLINVEGSGLPAATAVSVTVTLNGGAAQFTVNDVTDAAGYFALDF
                     GAVAGLPTQTRSVDNIVITIN"
     gene            complement(1713..>2944)
                     /locus_tag="PLQ54_09385"
     CDS             complement(1713..>2944)
                     /locus_tag="PLQ54_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOS93512.1"
                     /translation="RFPQGRLWLSTWLLDQVNAKGEYDGLLSYIERERPQWIDGIIVG
                     THSDWIPEPLLHRPEPDRYPLAAFPEISMYRMEPWGEHGANPLPGFCSRLADKLSGQV
                     IGGWPYSEGIYEDLNKFYWARFFWRPSAATDDILREYAVHYLGADVADDAVRLFRLME
                     QTHARSGWRVADLTGADEERRLAESINSRLPAWARVSWRWRILYIRAAIDGLLASEPS
                     IGPEAHTRLQALCDELVRIYHADGTFIRPPALPRLPDPRNLALGRPVTASSCQPGYGD
                     RGAHLTDGIRAEDDGENFWCHDPRQEETATLTVDIGRRMEVAEVNLQLRGLYGAFWFV
                     PESIDVSISDDGVVFRPVAGTREPPREGAPYSPEPVRIAIGQRARYVRVRLGRSQHQK
                     EPYAGVLELTELEVLGP"
BASE COUNT          511 a          994 c          954 g          485 t
ORIGIN      
        1 gctcattccg tcgaacagaa gatagatggt gttgagcccc gcctccaccg aggcagggct
       61 ccctccacaa ctcgatctcg tcgcgtcgct gcagctagtt gatggtgatg acgatgttgt
      121 cgaccgaacg ggtctgcgtg ggcaggccgg ccacggcgcc gaagtccagc gcgaagtagc
      181 ccgcggcatc cgtgacatca ttcacggtga actgcgcagc gccgccgttc agggtcaccg
      241 tgacgctcac cgcggtggcg gccggaaggc ccgagccctc aacgttgatc aggctggcga
      301 gctggttgta gacgggcacg ccgatctcga agacaacctc ggagatgttg gcgttggtgc
      361 cggtcagacg ggcggggctc gcatagttca ggcgaacgtt gcccaggaac tgctgcttgc
      421 gctggccgaa ggtcgtgctc tgctccagcg tgatcgcatt ggcggtgagc cagcccatgg
      481 cgccaggcag gacaggcagg cgaggacgat cgtagcccag ggtgttctcc tggcccggat
      541 cgataccggc cgactgcggg gcgacctgaa tgacaccgtt ctggttggtg atcgactgga
      601 acttggcgtt cttgttctcg taccagatcg catcctcggt ctcagggcag agctggttcg
      661 ccacagcgaa cacgcccgcc tgggccgcgg ccagggtacc ggccgacagg acatggaagg
      721 tggcgggcac gcccctcagg acgccgactc caccctcagg ctggagaacc aaagacgtga
      781 ggtagaactg gttgactccg gggttggcca catcgacgga caggaccgcg cgaacggtcg
      841 ccgccgtcgc ctcgttgctc tggccgggga cgatcgtgtt ggtgaggtcg cccgagccgg
      901 tgaagaacgg gctggtgcag cccgcaagcg ctacgaggat gcccacaccc aggaagaggg
      961 gcgcgcagcg aacgaaacta cgagtcaaga agaccacttc cctttcttgt cagtgttgcc
     1021 actcgttgct ccctgcgagt ccctcgggag catcctggtg cgcacagtcg gcgaggttat
     1081 cagaggccat ctaggctgtc aacgtccggg cgccttctcc gcggtgtgtc aaatcccgtt
     1141 atacggcgcc ccgaacaccc cacgacttgc cgaaatcggc accacgcgca catgcacaca
     1201 agtcgtcgaa gcccgttgtc cccctctcgc cgaacctgaa cgcgacaaga ggcgaacgag
     1261 gtgtgagctg acttcccccc ctccctggcg gacacccaca acgccttgct tcaccagccg
     1321 gcactcttgc ttccgcaagg ccttcagcca gcaagacaag gcgcacggcc gacctgggca
     1381 tgggagtccc ccgcaactgc caaacgcggc gccccgggcc caagttcagc cttcgcatcc
     1441 ttctgaccca tgctattccg gtcgtccctg ccaaggacct ccctcccttg cccagcgctt
     1501 tttttcgcgc ctgcggcctc gcgcgcccag gcttgcggtg gaagcccgag taccgtgtgc
     1561 tcatgtgcga gaagtccaac gaagggggtc cgtggagctc gccgctggct ccgcccatcc
     1621 gatgcgctct ggcgagttcg tcacggtcga gctgagcgac acgacctggg ccgacgtcgc
     1681 cggaaccgtc gagccggaga cgatgcccgc tcctacggcc cgagcacctc gagctccgtc
     1741 agttcgagta ccccggcgta cggctccttc tgatgctgcg agcggcccag gcggacgcga
     1801 acgtaccgcg ccctctggcc gatggcgatc cgaaccggct ccggcgagta cggcgcgccc
     1861 tcacgagggg gttcacgcgt ccccgccacc ggacggaaga ccacgccgtc atccgagatc
     1921 gacacgtcga tgctctccgg cacgaaccag aacgcgccat agaggccacg gagctgcagg
     1981 ttcacctcgg ccacctccat gcggcggccg atgtccacgg tcaatgtagc cgtctcctcc
     2041 tggcgtggat cgtggcacca gaagttctcg ccatcgtcct cggcccggat gccgtccgtc
     2101 aggtgagccc cgcggtcacc atagccaggc tggcacgacg atgcggtcac cggacggccc
     2161 aaggccaggt ttcgcggatc cggcaatcgt ggcagcgccg gagggcggat gaaggtgccg
     2221 tcggcgtggt agatgcgcac cagctcgtca cacagcgcct ggagccgggt atgagcctct
     2281 gggccgatcg acggctccga tgcgagcaga ccgtcgatgg ccgcacggat gtacaggatg
     2341 cgccagcgcc acgatacgcg tgcccatgcg ggcaagcggg agttgatgga ctcggccaac
     2401 cgccgctcct cgtccgcacc ggtcagatcg gccacacgcc agccgctccg tgcgtgcgtc
     2461 tgctccatga ggcggaacag gcggacggca tcgtcggcca cgtctgcgcc caggtaatga
     2521 acggcatact cgcgcaggat gtcgtcggtc gcggcgctgg gccgccagaa gaagcgtgcc
     2581 cagtagaact tgttcaggtc ctcgtagatg ccttccgagt agggccatcc gccgatgacc
     2641 tgcccgctaa gcttgtcagc caagcgcgag cagaagccgg gcagtgggtt ggccccgtgc
     2701 tcgccccatg gctccatgcg gtacatcgag atctcgggga aggccgccag cgggtaccgg
     2761 tcgggctcgg gccggtggag caagggctcc gggatccagt cggagtgggt gccgacgatg
     2821 atcccgtcga tccactgcgg cctctcccgc tctatgtagc tgagcaagcc gtcgtactcc
     2881 cctttggcgt tcacctggtc gagcagccac gtcgacagcc atagcctgcc ctgagggaag
     2941 cgcg
//
LOCUS       DAOSPL010000467         2942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_33693_length_2942_cov_3.186353, whole genome shotgun sequence.
ACCESSION   DAOSPL010000467 DAOSPL010000000
VERSION     DAOSPL010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33693_length_2942_cov_3.186353"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2085)
                     /locus_tag="PLQ54_09390"
     CDS             complement(<1..2085)
                     /locus_tag="PLQ54_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93513.1"
                     /translation="MRPIIAMALLWLVAPPAGAAPPPQVWPGFPEVTAAGARWRVLVA
                     VDGRGPAPVVSAPGAEVSVQRAPWTGDSQDVFLVHVEQAGFDHGPELVVECGGARARQ
                     ELPIGIDLSALPWEAWWAGAEVHAAVVRRPPAPDEGIWTPRVVPHTWEDLGVTWVRTR
                     VWIPEAWRAKSLVLDIGAIDDADVTFINGVSIGATEGWDRRRTYAIPPHVIRWDAENE
                     LCIAVDNRHAGGGLTRLPLALVPLGTRVSEAMPFENGARQREADRARPQALGPRLPLR
                     PMVVRGGVLEYADGGEVALWGVNIYPQSWVEFNTLKALGVDHYRALDEDMEDLSRMGV
                     DIIRIHVFDREISDGQGNLVRNIHLDLLDDIIRHCNERGIYLMLTPIAWWDSPNGRPD
                     SFSRAIPKEGLSMCEDARPIQRRYLRQFLTHTNPYTGRRLVDEPCLALFEIVNEPVYW
                     SLADMTGPGAAPSPALEGVRAAWERLAPDPGWRTPALFAWFRYHLVRSYIDDMIGAMR
                     ATGARQPIAYSSWAWQGPDLAAAVADSRCDAVTMSDYPGGLGQINDGVNLLGTQGNGD
                     VDTRFASKARLVYEFDAPGTVTKVHLYPAMARRWRNLGVQVACQFQYDARAAAHVNWD
                     WPQHYLNLWHTPGKAVSFMAAGEAFRRIPRGTEFEVGEDDQVFGQAAVSFGRNTALLV
                     APDCYMQAAPTDW"
     gene            complement(2233..>2942)
                     /locus_tag="PLQ54_09395"
     CDS             complement(2233..>2942)
                     /locus_tag="PLQ54_09395"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOS93514.1"
                     /translation="GLAIGATGKDSAIIEFPGYDGAGQPPIGAAARGVVALHLAPRGQ
                     GISAAHYHLPEGQQICLGLESPQTHYYGWAFCDAVRGVDLLESLSEVDARRIAAQGGS
                     QGGGLALALAALDPRVRVVAAAVPFLCGFERVIDARSDPLCRIARALATPGAPTRERA
                     LATLAHIDVVRLAPRIRCPVLMSIHDDDDVCPGVAIAAAFDCIPTRRMLIRYPGLGHC
                     GNIDFARRAWSWIEEHW"
BASE COUNT          460 a         1016 c         1028 g          438 t
ORIGIN      
        1 ccagtctgtc ggggcggctt gcatgtagca gtcgggcgcg accagaaggg ctgtgttccg
       61 accgaagctc accgcggcct ggccgaacac ctggtcgtcc tcgcccacct cgaactcggt
      121 gccccgaggg atgcggcgga aggcctcgcc ggccgccatg aagctcacgg ccttgcctgg
      181 tgtgtgccag aggttgaggt agtgctgggg ccagtcccag ttcacgtgtg ccgcagctcg
      241 cgcatcatac tggaactggc aggccacctg gacgcccagg ttccgccacc ggcgcgccat
      301 ggccgggtag aggtgcacct tcgtgaccgt gccgggggcg tcgaactcat agacgagccg
      361 ggccttcgag gcgaagcgag tgtcgacgtc gccattgccc tgggtgccca gcaggttcac
      421 gccatcgttg atctgcccga ggccgcccgg gtagtcgctc atcgtcaccg cgtcgcaccg
      481 gctgtccgcc acagccgccg ccagatccgg tccttgccag gcccaggagg agtaggcgat
      541 cggttggcgc gcaccggtgg cgcgcatcgc cccgatcatg tcgtcgatgt agctccgcac
      601 caggtggtag cggaaccagg cgaacagcgc cggcgtgcgc caacccggat cgggggccag
      661 ccgctcccac gccgcacgga caccctcgag tgcgggcgac ggcgcggccc ccgggccggt
      721 catatcggcc agcgaccagt agaccggctc gttgacgatc tcgaacagcg ccaggcaggg
      781 ctcatccacc agccgtcggc ccgtgtacgg gtttgtgtgg gtgaggaact gcctcaggta
      841 ccgccgctgg atgggtcgag cgtcctcgca catggacagc ccctctttgg ggatggcgcg
      901 tgagaagctg tcgggccggc cgttggggct gtcccaccac gcgatggggg tcagcatcag
      961 gtagatgccg cgttcgttgc agtggcggat gatgtcgtcc agcaggtcga ggtggatgtt
     1021 cctgacgagg ttgccctggc catcggagat ttcccggtcg aacacgtgga tgcggatgat
     1081 gtcgactccc atccgcgaca ggtcctccat gtcttcgtcg agggcccggt agtgatcgac
     1141 gcccagagct ttcagcgtgt tgaactcgac ccagctctgc gggtagatgt tcacgcccca
     1201 cagggccacc tcgccgccgt cggcatattc gagcacgccg ccgcggacga ccatgggccg
     1261 caacgggagc cgtggtccca gagcttgcgg ccgtgcgcga tcggcctcgc gctggcgggc
     1321 cccattctcg aagggcatgg cctcactgac ccgggtgccg aggggcacca gggccagcgg
     1381 cagccgcgtg agtccgccgc cggcgtggcg gttgtccacc gcgatgcaca gctcgttctc
     1441 agcatcccag cggatgacat ggggtgggat ggcgtaggta cggcgcctgt cccaaccctc
     1501 cgtcgccccg atgctcacgc cgttgatgaa agtcacgtcg gcgtcatcga tggcgccgat
     1561 atcgaggacc agggatttcg cgcgccacgc ctcggggatc cacacgcgtg ttcgcaccca
     1621 ggtcacaccg aggtcctccc aggtgtgtgg caccactcgc ggtgtccaga tgccctcgtc
     1681 tggcgccggg ggccgcctca caacggctgc gtgcacctcg gccccggccc accaggcctc
     1741 ccagggaagc gccgacaggt cgataccgat gggcagttcc tgccgcgcgc gcgcgccgcc
     1801 gcattccacg acgagttcgg gcccatggtc gaaccccgcc tgttccacgt ggacaaggaa
     1861 gacgtcctga ctgtctcccg tccacggcgc ccgctggacg gagacttcgg cgccaggcgc
     1921 cgagacaacc ggcgcgggcc ctcgcccgtc cacggccacg agcacgcgcc aacgggcgcc
     1981 ggcggcagtc acctcgggga agccgggcca cacctgcggc ggtggcgcgg ccccggccgg
     2041 gggggcaacg agccagagta gagccatggc gatgatggga cgcatcggct acctccgagc
     2101 gatctgcatg gatcccaatc agtgtgcacc gcccgagccc agcgcccaac tgctgtgtcc
     2161 gctcgccccg tcgattgcgg accggggagg gtcgaccggc accaccgcgg cgcttggcag
     2221 catcgttcta cctcaccagt gctcctcgat ccagctccat gcgcggcgcg cgaagtcgat
     2281 gttcccgcag tggccgagcc cgggatagcg aatgagcatc cgccgagtgg ggatacagtc
     2341 gaaggccgcc gcgatcgcca caccggggca gacgtcatcg tcatcgtgga tgctcatgag
     2401 cacggggcac cggattcggg gcgccaggcg gacgacatcg atgtgggcca gtgtggcgag
     2461 ggcccgctcg cgggtgggtg cgccgggcgt tgccagtgcc cgggcgatcc ggcagagggg
     2521 atccgaccgg gcatcgatga ctcgctcgaa cccgcacagg aagggcaccg cagccgccac
     2581 aacccgcacg cgagggtcga gggcggccag tgccagcgcg agcccgccgc cctgactgcc
     2641 gccctgggcg gcgatgcgcc gcgcatcgac ttcggagagc gattcgagga gatcaacgcc
     2701 gcggaccgca tcgcagaagg cccagccgta gtagtgagtc tgagggctct cgaggccgag
     2761 gcagatctgc tggccctcgg gcaggtgata gtgagccgcg ctgatgccct gccctcgcgg
     2821 ggccaggtgc agagcgacca cgccacgagc ggcggcgccg atcggcggct ggccggcacc
     2881 atcatagccg gggaactcga tgatggccga gtccttgccg gtcgcgccga tcgcgagacc
     2941 ct
//
LOCUS       DAOSPL010000468         2940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_33712_length_2940_cov_3.890468, whole genome shotgun sequence.
ACCESSION   DAOSPL010000468 DAOSPL010000000
VERSION     DAOSPL010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33712_length_2940_cov_3.890468"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PLQ54_09400"
     CDS             <1..820
                     /locus_tag="PLQ54_09400"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOS93515.1"
                     /translation="GIDAYMGMIGLNVVEPGRMALVMGSSTCHMCLCAHGVFGSNVWG
                     PFPDALTLGAWVLEGGQTATGSIVKWFADGFCGTYAAEAKARGQSIYALLDAEAARVP
                     IGSEGVVCVDYFQGNRTPLRDPLARGALWGLGLRHGPGHILRAIYEGTAFGTRHIVED
                     MAAAGFSPTEIYACGGGARSRLWLQIHADACGLPITLTEVQEAAALGSAILGAVAAGL
                     FPSIPEAAAQMVRTTDRVEPNAANRAEYDFYFDKYVRTYPALKDLMHEVAGHVV"
     gene            810..1592
                     /locus_tag="PLQ54_09405"
     CDS             810..1592
                     /locus_tag="PLQ54_09405"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOS93516.1"
                     /translation="MWSELAPIPDWIGRVVHLDRVGSTMDAARDLAFQPGAPAILVAA
                     EEQTAGRGRRGAVWHSVPGLGLAFSVLLRPVAAPEAVGLYSLAAAHAVAEAVRRLGVK
                     SATKWPNDVWARGRKLAGVLVESAIHEHRLAYVSIGIGLNANEAAHQLPEAPPRGATS
                     LRVELGAPVDRSLVGASVLDVLGGLLDARLAPRPEPIIDGWRRTDICLGHRLEVSLGA
                     EEFVCVGRDITQTGALIVEDDSGALRELVAGRVRVALVNRPA"
     gene            1631..2698
                     /locus_tag="PLQ54_09410"
     CDS             1631..2698
                     /locus_tag="PLQ54_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOS93517.1"
                     /translation="MSLPAEPDPALWSLLCEAVVVADSGGRVVFANAAAEELFGVRGE
                     PLIGRVAAELGTYVEDGGCPPFAFLAGGIPCEGVGRVLRIPGRPDAPVLVRQAALAGG
                     AGAEILSPAADCRGLLELRTDLGSTLNHDIRAAVSAVVLGIEAAQTLGGENLPRHVLA
                     FLERAQVACDRTLRLVSDAHDVARLEVGLTRLQREPVPLGRGIASGLEFVRGDADDAG
                     ITLDVRLHDVDRTVDADPDRFSRLVQNLAKAAVDASPRGGTVRIQSRDLPHGAIELAV
                     RLTVLDGVPEEASRAFDPFRSPEVRERLKRLGSPCAFAFCRAVAVAHGWAISALADGP
                     ELEYRVVMADPGPDEGGPSPC"
     gene            2754..>2940
                     /locus_tag="PLQ54_09415"
     CDS             2754..>2940
                     /locus_tag="PLQ54_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93518.1"
                     /translation="MTHRENWLRAIEFRGPEWIPCGIGFAPIVWRHYRGELERVLMDF
                     HKLFPGYRTNPLTLVIQS"
BASE COUNT          383 a          989 c         1050 g          518 t
ORIGIN      
        1 cggcatcgat gcctacatgg gcatgatcgg cctcaatgtc gtcgagccgg gccggatggc
       61 cctggtcatg gggtcgtcca cctgccacat gtgcctctgc gcccacggtg tgttcggctc
      121 gaacgtgtgg gggccgttcc cggatgccct cacgctgggc gcgtgggtgc tcgaaggcgg
      181 ccagacggcc accggctcca tcgtgaagtg gttcgccgat ggcttctgcg gtacctatgc
      241 cgcggaggcc aaggcccgcg gccagagcat ctacgcactc ctcgacgccg aggccgctcg
      301 agtaccgatc ggcagtgaag gcgtggtgtg cgtggactac ttccagggca accgcacgcc
      361 gttgcgtgat cctctggccc ggggggcgct gtggggcctg gggttgcggc acggccccgg
      421 gcacatcctc cgggccatct acgaggggac cgccttcggc acgcgccaca tcgtcgagga
      481 tatggcggcg gcgggtttct cgcctacgga gatctacgcg tgcggcggcg gggcgcggag
      541 ccggttgtgg ctgcagatcc acgccgatgc ctgtgggctg cccatcaccc tcaccgaggt
      601 ccaggaggcg gcggcgctgg gttccgcaat cctcggtgcc gtggcggccg gcctgttccc
      661 gtccatcccc gaggccgcag cccagatggt gcgtaccacg gaccgtgtcg aacccaatgc
      721 cgcgaaccgc gccgagtatg acttctactt cgacaagtac gtccgcacct atcccgctct
      781 gaaagacctg atgcacgaag tggcgggcca tgtggtctga gctggctccg atcccggact
      841 ggatcgggcg agtggtgcac ctggatcgcg tgggctcgac gatggacgcg gctcgagatc
      901 tggccttcca gccgggtgcg ccggcgatcc tcgtcgctgc cgaggaacag accgcgggcc
      961 gcgggcggcg aggtgcggtc tggcacagcg tcccgggcct cgggctcgcg ttcagtgtgc
     1021 tcttgcggcc cgtggcagcc cccgaggccg tgggcctcta ctcgctggcc gcggcccatg
     1081 ccgtggccga ggccgttcga cggctcggag tgaagtctgc caccaagtgg cccaacgacg
     1141 tgtgggcgcg tggccgcaag cttgccggag tcctcgtgga atccgcgatc cacgagcatc
     1201 ggctcgccta tgtgagtatc ggtatcgggc tgaacgcgaa cgaggcggct caccagcttc
     1261 cggaggcccc gcctcgcggc gccacctccc tccgggttga actcggtgca ccggtggacc
     1321 gaagcctggt gggcgcctcg gtgctggatg tgctgggcgg cctgctggat gcgcggctcg
     1381 ctccgcgccc ggagcccatc atcgatgggt ggcgtcgaac cgacatctgt ctcggacacc
     1441 ggctcgaagt gtccctgggg gcggaggagt tcgtctgtgt ggggcgggat attacccaga
     1501 ccggcgccct gatcgtcgag gacgatagtg gggcgctccg tgagctcgtg gcgggccgcg
     1561 tccgcgtggc tctggtgaac agaccggcct gaggttcgtg gtcttgcggt gcccttgggg
     1621 gtgaacgctc gtgtcactgc cggctgagcc tgacccggcc ctgtggtcgc tgttgtgcga
     1681 ggccgttgtt gtggccgact cgggcggccg cgtcgtgttc gccaacgcgg ccgccgagga
     1741 gctcttcggc gtgcgtgggg agcccctgat cgggcgagtg gccgccgagc tcggcaccta
     1801 cgtcgaggac ggcggatgcc cgcccttcgc tttcctggcg ggtggcatcc cttgcgaggg
     1861 cgtgggccgc gtgctccgaa tcccgggccg gcccgatgca cccgtgctcg tccgccaggc
     1921 ggcgcttgcg ggcggagccg gcgccgagat cctctcgccc gccgccgatt gtcgaggcct
     1981 tctggagctc cgcaccgacc ttggtagcac cctcaaccac gacattcgtg cagcggtgtc
     2041 cgcagtagtg ctcggcatcg aggccgctca gaccctgggt ggcgagaacc tgcctcgcca
     2101 cgtgcttgca tttctggagc gcgctcaggt ggcctgcgac cgcacgctcc gcctggtcag
     2161 tgacgcgcac gacgtggcac gcctggaggt gggcttgact cgcctccagc gcgaacccgt
     2221 gccccttggg cgggggatcg cgtctgggct cgagttcgtc cgaggtgatg cggatgacgc
     2281 tggcatcacg ctcgatgttc gcctgcacga tgtcgaccgg acagtggatg ctgaccccga
     2341 tcggttctca cgactggtgc agaacctggc caaggccgcg gtggacgcgt cgccacgtgg
     2401 cggtaccgtg cggatccagt ctcgcgatct accccatggc gccatcgagc tggccgttcg
     2461 cctcaccgtg ctcgacgggg tgcctgagga agcatcgcgc gccttcgacc ccttccgctc
     2521 ccccgaggtg cgtgaacggc tcaagcgact cggatccccc tgtgcctttg ccttctgccg
     2581 agccgttgct gtggcgcacg gctgggccat atcggccctg gccgatggcc cggaactcga
     2641 gtaccgcgtc gtgatggccg atccggggcc cgacgagggc gggccatcgc cgtgctgagg
     2701 gggggtcggc tccgccatac gggtgcgatc gtccgcaccg ggggccgctc gccatgacac
     2761 atcgcgagaa ctggctgcgc gccatcgagt tccgcgggcc cgagtggatc ccgtgcggga
     2821 tcggcttcgc gcccattgtg tggagacatt accgcggaga gctcgaacgg gtcctcatgg
     2881 acttccacaa gctcttcccc ggctaccgga cgaacccgct gacgctggtg atccagtcga
//
LOCUS       DAOSPL010000469         2937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_33747_length_2937_cov_3.677307, whole genome shotgun sequence.
ACCESSION   DAOSPL010000469 DAOSPL010000000
VERSION     DAOSPL010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33747_length_2937_cov_3.677307"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="PLQ54_09420"
     CDS             complement(<1..1467)
                     /locus_tag="PLQ54_09420"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOS93519.1"
                     /translation="MVSITIGCTCAGAFAAPEEPRSDWFGYERLDFVINGRDCLLVLP
                     KRAAEGKPWIWRMEFFGHEPQADLALLEHGFHVAYMDVQNMYGAPVALDHMDAFYDHL
                     TGPWGLSPKAVLEGFSRGGLFSFNWAARHPDRVACIYNDAPVCDFKSWPAGKGRAQEH
                     AEDWQRLLDVYGLTEEDALAYTGNPIDNLAPLAAAKIPLLHVCGEVDTGVPIEENTRI
                     VEARYRELGGEILVISKPNCDHHPHSLQDPTRIVNFILAHVPGLPDVPIREPQTPYGY
                     DYFVLRSGLGNCRIRFVTEKRGRVAFLGGSITAGGGWRDMVCEELQRRFPQTEFDFIN
                     AGIPSYGSTPDAFRFARDVLKNGTVDLLFAEAAVNDDTNAFPPVEQVRGMEGIVRQAR
                     LANPEMDIVLLHFADPGKVAETLAGRTPEVIVSHERVAEHYAVPSINLAREVAERIHA
                     GEFSWEKDFVDLHPSPFGHGVYFRSICRLFDAAWPQPMA"
     gene            1845..>2937
                     /locus_tag="PLQ54_09425"
     CDS             1845..>2937
                     /locus_tag="PLQ54_09425"
                     /inference="COORDINATES: protein motif:HMM:NF015387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOS93520.1"
                     /translation="MIRLAGVLLLASSAYGAEFHVATDGNDANPGTKAEPFRTIQRAA
                     DLARPGDIVTVHEGVYRERVNPPRGGESEAKRIVYRAAPGERVEIRGSEVIRGWVRER
                     GDVWKVTLSNAFFGGFNPYADVIHGDWFDPQGREHHTGAVYLDGDWLTEAASLDEVMM
                     PGGTKPAWLTAASRQTLLNVAWLRPGGAGKGERTPAAGFTAQRGIQTAPCTEGGECIG
                     WIEEGDWARYEGIELGQRTEAMELRVASATSGGLIEIRLGTPEGELLGMVDVPTTGDW
                     QRWISVVAPMKPLGGKRDLCLTFRARGTEEERLAARRLNRQLWFARVDAAHTTIWAQF
                     EGVDPNQRLVEINVRQSVFYPDQPGRDYIT"
BASE COUNT          483 a          940 c         1069 g          445 t
ORIGIN      
        1 ggccatcggc tggggccatg cggcatcgaa cagccggcaa atggagcgga agtagacccc
       61 atggccgaat gggctcgggt ggaggtcgac gaagtctttc tcccaggaga actcgcccgc
      121 atggatccgc tcggccacct cgcgggccag gttgatcgac gggaccgcat agtgctcggc
      181 gacgcgctcg tggctgacga tgacctcggg cgtccggccg gcgagggtct cagccacctt
      241 gccggggtcg gcgaagtgca gcagcacgat atccatctcc gggttggcga gcctcgcctg
      301 gcggacgatg ccctccatcc cacgcacctg ctcgaccggt gggaaggcgt tcgtgtcgtc
      361 gttgacggcg gcttcggcga acaggagatc gaccgtaccg ttcttgagca catcgcgggc
      421 gaagcggaag gcatccggcg tcgacccgta ggacggaatg cccgcgttga tgaagtcgaa
      481 ctccgtctgt gggaaccgcc gctggagctc ctcacacacc atgtcgcgcc agccgccgcc
      541 agcggtgatc gatccgccca ggaaggccac gcggccacgc ttctccgtga cgaaccggat
      601 gcggcagttg cccaggccgc tccggagaac gaagtagtca tagccgtagg gggtctgcgg
      661 ctcgcggatg ggcacatcgg gcaaaccggg cacgtgcgcg aggatgaagt tcacgattcg
      721 cgtgggatcc tgcagactgt gcgggtggtg gtcgcagttg ggcttcgaga tcaccaagat
      781 ctcgccgccg agctcccggt accgcgcctc gacgatccgc gtgttctcct cgatgggcac
      841 tcccgtgtcg acctcgccgc agacgtgtag caacgggatc ttggcggcgg ccagcggcgc
      901 caggttgtcg atggggttgc ccgtgtaggc caaggcatcc tcttcggtga ggccatagac
      961 atccagcagc cgctgccagt cctcggcgtg ctcctgcgct ctgcccttgc cggcaggcca
     1021 gctcttgaag tcgcacacgg gggcgtcatt gtagatgcag gccacgcggt cggggtggcg
     1081 cgccgcccag ttgaacgaga agagcccgcc gcggctgaat ccctcgagca ccgccttcgg
     1141 cgacagaccc cacggcccgg tgagatggtc gtagaaggca tccatatggt ccagggccac
     1201 cggggcgcca tacatgttct gcacatccat gtaggcgacg tggaagccgt gctcgagcag
     1261 ggcgaggtcg gcctggggct cgtggccgaa gaactccatc cgccagatcc agggcttgcc
     1321 ctcggcagcg cgcttgggga ggacgaggag acagtcacgg ccgttgatga cgaagtcgag
     1381 ccgctcataa ccgaaccagt cggaccgcgg ctcctctggg gcggcgaatg cgcccgcgca
     1441 tgtgcacccg attgtaatac tcaccatcag cccacgcatt ccggtctccg tctgcggcgg
     1501 tgtcgatcag ccgatcacag tccgttcggc ggggaggagg catgacctgc cggcggccca
     1561 cggagcagcg cgcagtctgg gcgtccacgt ccgacaccgc cgcggcgagc tccccaggcc
     1621 tcatcgccgc cgcccttggt cacgccgcgt ggacgcggcg gctcagggtc ggcgggttgg
     1681 ggcgtgcggg gcacgggcgt ctctggcccg ctgcccttcg tcatgccgcg cgccacccgc
     1741 ggcgctcggg ccgaatagaa gggtccagcg cgccggccgg gaagcacacc acgaggccga
     1801 gggtgctgag gccgggcgag agggtgagtt cgatagggca cgcgatgatt cggcttgcgg
     1861 gtgtgctcct gttggcgagc agcgcctacg gcgctgagtt ccacgtggcg acggacggga
     1921 acgacgccaa ccccggcacg aaagcggaac cgtttcggac cattcagcgc gcggccgatc
     1981 tggcccggcc gggcgacatc gtcacggtgc acgagggagt ctaccgggag cgggtgaacc
     2041 cgccgcgggg cggcgagtcc gaggccaagc gcatcgtcta ccgcgccgcc cccggcgaga
     2101 gagtcgagat ccggggctcg gaggtcatca ggggctgggt acgcgagcgg ggcgacgtct
     2161 ggaaggtcac actatcgaac gccttcttcg gcggcttcaa cccttacgcc gacgtgatcc
     2221 acggcgactg gttcgaccct caggggcgtg agcaccacac gggcgccgtg tacctggatg
     2281 gcgactggct taccgaggcg gcgagtctcg acgaagtgat gatgcccggc ggaacgaaac
     2341 ccgcgtggct gacggccgcg agccggcaga cgcttctgaa cgtcgcctgg ctgcgtccgg
     2401 gtggagcggg caagggcgag cgcactccgg cggccggctt cacggcacag cgggggattc
     2461 agaccgcgcc gtgcaccgag gggggagagt gcatcggctg gattgaggaa ggtgactggg
     2521 cgcgctacga ggggatcgag ctggggcagc ggaccgaggc gatggagctc cgcgtggcgt
     2581 cggcgacgtc ggggggcctc atcgagatcc gtctgggcac gccggagggc gaactgctgg
     2641 gcatggtgga tgttcccacc accggcgact ggcagcgatg gatctctgtg gtcgccccga
     2701 tgaagccgct gggcggcaag cgggacctgt gcctcacgtt ccgggcacgg ggtaccgagg
     2761 aggagcgcct ggcggcgcgt cgcctgaacc ggcagctgtg gttcgcgaga gtcgacgccg
     2821 cacacaccac catttgggcg cagtttgagg gcgtggaccc caaccagaga ctggtcgaga
     2881 tcaatgtgcg ccagtcggtg ttctacccgg accagccggg gcgcgactac atcacgg
//
LOCUS       DAOSPL010000470         2933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_33807_length_2933_cov_3.445101, whole genome shotgun sequence.
ACCESSION   DAOSPL010000470 DAOSPL010000000
VERSION     DAOSPL010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33807_length_2933_cov_3.445101"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="PLQ54_09430"
     CDS             complement(<1..1253)
                     /locus_tag="PLQ54_09430"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOS93521.1"
                     /translation="MTTAPGLPTAGGSAAADGGHAPPALDAHHISKRFPGVQALTDVS
                     LVIEPGTVHALVGENGAGKSTLVKIIAGLQSPDSGTIEVHGTRMTRFSPIAARDLGVA
                     LVAQEPEHFADLTALENLFVGRWPREAGLISWPAMERQARAMLEQMGAEIDLSARMGD
                     LALAHRQVIQIARALLQNAKVLILDEPTAALGQEETRALFDMVRRLSGQGVAIVYISH
                     RLEEIFEIAQQVTVLRDGRHVVTCATTQVSRDDLVRHMVGTEVAEEYSHTPADEGPGP
                     DVPPVLALEGVTSPAGFADVSLDVRPGEVLGLAGLAGSGRNELAHALGGALPIASGRV
                     VVNGETRRLKGPDGARRLGLVLAPGDRPGRALVLPMTVRENITLSLLRELRRGPLTNV
                     RAESALADEYIGKLDIRTPSAEQAT"
     gene            complement(1393..>2933)
                     /locus_tag="PLQ54_09435"
     CDS             complement(1393..>2933)
                     /locus_tag="PLQ54_09435"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93522.1"
                     /translation="FGERWHVYTLHRMSPDGSNLRGLSVHETNEWFPEVLADGSLAYA
                     RWDYVDRHAVLHQNLWRTHPDGTNPVALWGNHTQNPHCSFEGKAVPGSRKILCTASAH
                     HSITAGSLILIDPARAVDGLDAVERVTPDVCFPEAEGWPSSYYATPWPLSEDFYLASY
                     SPRPLIPEPSPNDPAALGLYLVDRWGNRELLYRDPAIGSTNPIPLRARPRPPVLPDRF
                     PLGTEPAPTGQYLVLDVYRGLTGVSAGAVKALRIVQILPKATPVADAPPVGLAGQEPT
                     KAVLGTVPVEPDGSAYFEAPARKPLLFQALDERGMALQTMRSAAYLQPGETLSCVGCH
                     EARTTSPPAPMPRAARRGPSAIRPGPDGTAPFSFARLVQPVLDRHCVGCHPAGGGQTA
                     VDLSGTPSGAFSASYMALTGGTTSFYADGTNAENARAALVPRYGGWNPVHTTAVGGSY
                     GARGSRLMRMLDEGHYGVTLPPDDWERLAIWVDANALFYGTYAVEDQRRQLAGEAIAM
                     PELQ"
BASE COUNT          445 a         1052 c         1017 g          419 t
ORIGIN      
        1 taggtcgcct gctcggccga tggggtgcgg atgtcgagtt tgccgatgta ctcgtcggcc
       61 agcgcggact cggcccggac gttggtcagc ggcccgcggc ggagctcccg caggagtgag
      121 agcgtgatgt tctcccgcac ggtcatcggg agcaccagcg cgcgcccggg ccgatcgccc
      181 ggagcgagca ccaatcccag ccggcgcgcg ccgtccggcc ccttcagccg acgggtctcc
      241 ccgttcacga ccacgcggcc ggacgcgatc ggcagcgcgc caccaagcgc gtgggcgagc
      301 tcgttccggc cggaacccgc caggccggcc agtccgagca cctccccggg gcgcacatcc
      361 agcgagacat cggcgaaccc ggccggcgag gtcacgcctt ccagcgcgag gacggggggc
      421 acatcgggcc cggggccctc gtcggcaggt gtgtgcgagt attcctcggc cacctcggtg
      481 cccaccatgt ggcggaccag gtcgtcgcgc gagacctgcg tggtcgcgca ggtgaccacg
      541 tgccggccgt cgcgcagcac ggtgacctgc tgcgcgatct cgaagatctc ctcgagccgg
      601 tggctgatat agacgatggc cacgccctgg cccgagagcc tccgcaccat gtcgaacagc
      661 gcccgcgtct cctcctgccc cagggccgcg gtgggctcgt cgaggatcag caccttggcg
      721 ttctggagca gcgcccgcgc gatctggatg acctgccgat gcgccagggc caggtcgccc
      781 atgcgggcgg agaggtcgat ctctgccccc atctgctcga gcatggcccg ggcctgccgc
      841 tccatggcgg gccatgagat gagccccgcc tcgcgcggcc agcgaccgac gaagaggttc
      901 tcgagcgccg tcaggtcggc gaagtgctcg ggctcctgcg ccaccagcgc gacacccaga
      961 tcgcgggccg cgatgggcga gaaccgggtc atgcgagtac cgtggacctc gatggtgccc
     1021 gagtcggggc tctgcaggcc ggcgatgatc ttgaccagcg tcgacttgcc cgcgccgttc
     1081 tccccgacca gggcgtggac ggtgcccggc tcgatcacca gcgacacgtc ggtgagcgcc
     1141 tgaaccccgg ggaagcgctt cgatatgtga tgtgcatcca gcgccggcgg ggcatggccc
     1201 ccgtcggcag cggccgagcc gccggctgtc ggcaatccgg gtgcggtggt caacgcgtgt
     1261 ggctctccat cgggtactgc gcggcacagg atagcagacc ggcaaccgga ggccaaggag
     1321 cggcgccatg ccatggccca tcgcccaccc gcgtcgacgg ccggctgccc cggcccacac
     1381 gaatccccgg ccctactgca actccggcat ggcgatggcc tcgccggcca gctggcgtcg
     1441 ctggtcctcg acggcgtagg tgccgtagaa cagggcgttc gcgtcgaccc agatggcgag
     1501 ccgctcccag tcgtcgggtg gcaacgtcac cccgtagtgt ccctcatcca gcatccgcat
     1561 gagccggctg ccgcgcgcgc catagctccc ccccacagcc gtcgtgtgca ccgggttcca
     1621 tcccccgtaa cgtgggacca gggccgctcg ggcgttctcc gcattggtcc cgtcggcgta
     1681 gaagctcgtc gtgccccccg tcagagccat gtaggaagcc gagaaagcac cagagggcgt
     1741 tccgctcaga tcgacggccg tctgaccgcc gcccgcggga tggcatccca cacagtggcg
     1801 gtcgagcacc ggctgaacga gccgggcgaa ggaaaacggg gccgttccat cgggcccggg
     1861 ccggatcgct gagggccctc gccgcgccgc ccgcggcatg ggcgccgggg gcgatgtcgt
     1921 ccgcgcctcg tggcagccga cgcacgacag ggtctctccg ggctgcaggt acgccgcgct
     1981 ccgcatggtc tgcagtgcca taccgcgctc atcgagcgcc tggaacagca atggcttcct
     2041 cgccggcgcc tcgaagtacg ccgagccgtc cggctccacc ggcaccgtgc ccagcacggc
     2101 cttggtgggc tcctgaccgg ccaagcccac cggtggcgcg tcggccaccg gcgtcgcctt
     2161 gggcaggatc tgaacaatcc gcagggcctt cacggctccc gcgctgaccc cagtcagccc
     2221 acggtagaca tcgagcacca ggtactgccc cgtgggagcg ggttcagtgc ccaatgggaa
     2281 ccggtcgggc agcaccggcg gccgcggcct ggccctcaag gggatcgggt tggtgctgcc
     2341 gatggccgga tcccggtaca acagctcgcg gttgccccat cggtcgacca gatacagccc
     2401 cagcgcggcc ggatcattcg gcgacggctc ggggatgagc ggcctcgggc tgtagctggc
     2461 caggtagaaa tcctccgaca gcggccacgg cgtggcgtag taggaggacg gccagccctc
     2521 ggcctcgggg aaacacacat cgggtgtgac ccgctcaacg gcgtccaggc cgtccaccgc
     2581 gcgggcggga tcaatcagga tcagcgaccc ggccgtgatc gagtggtgcg ccgaagccgt
     2641 gcacaggatc ttccgcgagc ccggaaccgc cttcccctcg aacgagcagt gcgggttctg
     2701 cgtgtggttg ccccacaggg ccaccggatt cgtgccatcg gggtgggttc gccagaggtt
     2761 ctggtggagc accgcgtgac ggtctacgta atcccaccgg gcataggcca gcgatccgtc
     2821 ggccagcacc tcagggaacc actcgttggt ctcgtggacc gagagaccgc gaaggttgct
     2881 gccatcgggg ctcatccggt gcaaggtgta gacatgccag cgctcaccaa act
//
LOCUS       DAOSPL010000471         2931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_33839_length_2931_cov_3.081363, whole genome shotgun sequence.
ACCESSION   DAOSPL010000471 DAOSPL010000000
VERSION     DAOSPL010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33839_length_2931_cov_3.081363"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..516
                     /locus_tag="PLQ54_09440"
     CDS             178..516
                     /locus_tag="PLQ54_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93523.1"
                     /translation="MSQTVRVYGSMNMGLTADNVVSGATKLTEATIPAGAQNLSVSDQ
                     LDSEALAFLSSALQASPPVFAVYIVVEGDDPSALSFSSITLVPGAGQFSILSIRAVPG
                     ATPEIEIRIL"
     gene            complement(578..1921)
                     /locus_tag="PLQ54_09445"
     CDS             complement(578..1921)
                     /locus_tag="PLQ54_09445"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOS93524.1"
                     /translation="MRVVRHPLVVAAAAALALPATAQTPDAVDGSTWAIVIGIQVHED
                     DTLIPSRKGAYQTANAIYAYIADPARGGIGADHTRLLCEFGADRRSIQAAFEEVRGLA
                     KDSDRLLVYAGLRTALAADADGKITGYFLPYDAKLDSLATTAIAPADLAAWISAVPAR
                     RRVVFLDAAFADAIRAPGAENAAPPADYLNLLGASAAVIAATDGAKPVPEDGLEHPLL
                     GFSLYTAMQGPGDKNRDGKLTFAEFLQHHGDTVAAVYQGQEGNAPHPYSNGVEPEQVL
                     AAVPQADPRCPDGTRLVGEVCMDMYESPNKPGAPVYNPGNWFGAVAVCQQAGKRLCST
                     QEWMLACQGPGWKYPYGNEYIDSYCNVGDTEGHLERVGSRPFCVSAWGIYDLIGNAQE
                     WAGTEKKENATVLGGRFFDAPGESIGCRESLVAGNPILEGAELGMRCCIEPKPTE"
     gene            2143..>2931
                     /locus_tag="PLQ54_09450"
     CDS             2143..>2931
                     /locus_tag="PLQ54_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93525.1"
                     /translation="MRSRGEVAVLGAVTVWLLLAPAGAQTPPDAEIVVDLLEFAADCA
                     ARGSAGQVIPELTGAAVAGASLGGHDGVSVARSSVADAAVRDAVLGVACERLAYAAPQ
                     AALPLAQAIESPSGRDRALARIALPGAAGTSEVRLQALGCLGVPAAGAEAALGLARWP
                     EGPTGAEALTLLRYIPEEARQSAALAALAARGAVRDPKAIQRLVQAFSDPESRVCGLI
                     ALVPVKRQAADNSARKDLDEAVALLDRVRDKGRQAELRREVAVAL"
BASE COUNT          427 a          943 c         1062 g          499 t
ORIGIN      
        1 gaccaccaga ttggaaaagc tggcgctgcg ctcggcttga gcgttgaaca gcttgatcgc
       61 ctgcgcgccg cgcagcgatt cgaggaaatg actgttctgc cgtgcctcgt gcaccaaagc
      121 ctcttccgag gcctcacgtc ggcctcgttc tcgatggtcg cgacgaacag agcggccgtg
      181 agccagaccg tccgcgtcta cgggtccatg aacatgggcc tgacggccga caatgtggtg
      241 tcgggtgcca cgaagctcac cgaggcaacc atcccggctg gagctcagaa cctgtcggtc
      301 tcggaccagc tggactcgga agccctggcc ttcctgagtt cggccctgca ggccagccca
      361 ccggtttttg cggtgtacat cgttgtcgaa ggagacgatc catcggcgct gagcttcagc
      421 agcatcaccc tcgtccccgg ggccggacag ttctccattc tgagcatccg ggcggtgcca
      481 ggagccactc cggagatcga gatccgcatc ttgtgatcgg gttcgtcatc gtgcgccgcc
      541 cgggctccag gggcgagtgg cccggggctc ccggccgcta ctccgtcggc ttgggttcga
      601 tgcagcagcg catgcccagc tcggcgccct cgaggatcgg gttcccggcg accagggact
      661 cgcggcagcc gatgctctcg cccggcgcat cgaagaaccg gccgcccaga accgtagcgt
      721 tttccttctt ctcggttccg gcccactcct gagcattgcc gatgaggtca tagatgcccc
      781 acgcactgac acagaacggt cgggagccga cgcgctcgag gtggccttcg gtgtcgccca
      841 cgttgcagta gctgtcgata tactcgttgc cgtaggggta cttccacccc gggccctggc
      901 atgccagcat ccattcctga gtcgagcaca ggcgcttacc ggcttgctgg cagacggcca
      961 ctgcgccgaa ccaattgccc gggttgtaga cgggggcccc tggtttgttg ggggattcgt
     1021 acatgtccat acagacctct ccgaccaggc gcgtgccgtc ggggcagcgc ggatcggcct
     1081 gggggacggc ggccagcacc tgctcgggtt cgacgccgtt cgagtaggga tggggtgcgt
     1141 tgccctcttg tccctggtag acggccgcca cggtgtcgcc atggtgctgg aggaactcag
     1201 cgaaggtgag tttgccatcc cggttcttgt cgccgggccc ctgcatggcg gtgtagaggg
     1261 agaaaccgag cagtgggtgc tcgagcccgt cctcggggac gggcttggcg ccgtcggtgg
     1321 ccgcgataac ggcggcactc gcgcccagca gattcaggta gtcggccggc ggagccgcgt
     1381 tctcggctcc gggtgctctg atggcatcgg cgaacgcggc atcgaggaag accacgcgcc
     1441 tcctggccgg taccgcggag atccaggcgg ccaggtcagc gggcgcgatg gccgtagtgg
     1501 ccaggctgtc gagcttagcg tcgtagggca aaaagtaacc ggttatcttt ccgtcggcgt
     1561 cggcggcgag cgccgtgcgc agaccggcgt agaccagcag ccgatcggag tccttggcga
     1621 gccccctgac ctcctcgaac gccgcctgga tcgatctgcg atcggccccg aactcgcaca
     1681 acaggcgggt gtgatccgct ccgatgccac cccgggccgg gtcggcgatg taggcgtaga
     1741 tggcgttggc ggtctgatag gcacccttgc ggctggggat gagcgtgtcg tcctcgtgga
     1801 cctggatccc gatgacgatg gcccacgtgg atccgtccac agcatcgggc gtctgggcag
     1861 ttgccggaag tgccagggcc gcggccgcgg cgacgacgag cgggtggcgg acgactcgca
     1921 tctcgtgggc ctccaaatcg gggctgcagt ggagcggcgc gccagaactg cacatccgca
     1981 gtctctctga cacccggcgg cgcgtaacgt tccccgatgg tgagacgaga accgcgaccc
     2041 ggcagttcgg caccgcgagt gacggtccct gcgcggcctg gacgcaggtt cggcgcgggc
     2101 tccggcgaag gcagaacgac tatggtgagg caagaccttc gagtgcgctc gagaggggaa
     2161 gtggccgtgc tgggcgcggt caccgtctgg ctcctgctgg cgccagccgg cgcgcagacg
     2221 ccgccggatg cggagatcgt ggtcgacctc ctggagttcg ccgccgactg tgccgcccgt
     2281 ggatcagcgg gtcaggtgat accggagctg accggggcgg ctgttgcagg ggcctccttg
     2341 ggcgggcacg atggtgtctc ggtggcccgt tcgtcggtgg ccgacgcggc cgttcgtgat
     2401 gccgtgttgg gcgtggcctg tgagcgtctg gcctatgcgg cgccgcaggc agcgttgccg
     2461 ctcgcgcagg caatcgagtc gccgtccggg cgtgaccggg cgctggctcg gatcgctctg
     2521 cctggcgcgg cgggcacctc ggaggtccgg ttgcaggcgc tcggctgtct tggcgttccc
     2581 gcggccgggg cggaggcggc cctcgggctc gcccggtggc ccgagggacc gacaggggcg
     2641 gaggccctga cgctgctgcg gtacatcccg gaggaggcgc ggcaatcggc cgcgctggcc
     2701 gcgctggccg caagaggcgc cgtgcgcgac ccgaaagcaa tccagagact cgtacaggcg
     2761 ttctcggacc ctgagtcacg cgtctgtggc ctgatcgccc tggttccggt gaaacggcag
     2821 gccgctgaca actcggctcg caaggacctc gatgaggccg tcgcgttgct ggaccgggtg
     2881 agggataagg gccggcaggc ggagctgcgc cgcgaggtgg cggtggccct g
//
LOCUS       DAOSPL010000472         2927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_33910_length_2927_cov_3.286908, whole genome shotgun sequence.
ACCESSION   DAOSPL010000472 DAOSPL010000000
VERSION     DAOSPL010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33910_length_2927_cov_3.286908"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLQ54_09455"
     CDS             <1..393
                     /locus_tag="PLQ54_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93526.1"
                     /translation="LAAVEAFPDGTHVRRAEGGLLVATNYSVCRGIPERHEGRHGEAE
                     RLLSGRQQPLTAQDARAIVASHDGPFCAGRHAGAIDEFRDPDWYTLWGLVAEPAQRRL
                     SLAPGQPCRTPWVELPVGRDVEGNPGCH"
     gene            384..1064
                     /locus_tag="PLQ54_09460"
     CDS             384..1064
                     /locus_tag="PLQ54_09460"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HOS93527.1"
                     /translation="MPLTGRHVLITSGPTQAPIDAVRYIANTSSGALGAHTADAALDA
                     GASVLLVHGPGSIVPQPHPRLTLSPVRTVDDLIATLQRQLAATRFDAIAHAMAVLDYV
                     PEAAGTGKIPSGQDHIDLRLVPTPKVIDRVREWAPDSLLIGFKLVAGESDEGLRQAAL
                     TLLRRSGADLVVANDMVQIQAGEHRAVFIEPSGRTAGAFVGKAAIAEALVREIAARLA
                     GREGSGLS"
     gene            1061..1609
                     /gene="coaBC"
                     /locus_tag="PLQ54_09465"
     CDS             1061..1609
                     /gene="coaBC"
                     /locus_tag="PLQ54_09465"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOS93528.1"
                     /translation="MSHLEGRSVLVGITGGIACYKAAELVRSLRKAQAQVRVMMTRAA
                     CEFVTPLTFQTLSQKPVATDLFAPVSDWEIEHISLARGADVVVVAPATANVIGKVAHG
                     IADDLVTTVIMATRAPVVFCPAMNVAMWENPIVQANIRRLAELGYRFVEPAEGDLACG
                     EEGKGRLAEVERIVEAVAVALP"
     gene            1632..>2927
                     /locus_tag="PLQ54_09470"
     CDS             1632..>2927
                     /locus_tag="PLQ54_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOS93529.1"
                     /translation="MLSPTVRCAALIPIVVVCSDAWAAPSLHDQVEADWRLQDQSRMR
                     HIEQPGLVRFVEGEVQWPGAQPSQGARVPFGEAPRLDGLLDDACWAAAMVVEPAAPNL
                     PRFRLAHDGERLYIGAEMPSSTEAWYRGEPTGMDAGGAVDGVRDGRYGFHTGGDTDPW
                     WQVDLGRTTELGRLVVYNRLDYAPGLHNADQLRVLLSDDGTGWREVYQNPGSFFGGMD
                     DTGPLRVDLHGQRARYVRLQVPGAVLFHLDEVEVYGPDGIGPNLALGQPAQQSSLSIW
                     SRGGPSGGVLVQLGGTTVVLPSGQPPQAVTDGQAKQTGAAARGGGTTRLELALPLTQV
                     GAAYPGAARLASAEAIPLPLGARFDARLPDAPVLGYGIIRVPVAVGTVRGPWPGPVEL
                     SAELIVFTPRRMERVPGRVVTIDRPGTTEVTVTARHEGPA"
BASE COUNT          437 a         1021 c         1039 g          430 t
ORIGIN      
        1 ttggcggccg tcgaggcgtt cccggacggg actcatgtcc ggcgcgcgga gggcgggctc
       61 ctggtcgcga ccaactactc ggtgtgtcga ggcattcccg agcgccacga ggggcgccat
      121 ggagaagccg agcgcctgct atccggcagg cagcagccgc tcacggccca agacgcccgt
      181 gcgatagtcg cgagccacga tgggccattc tgcgcgggga ggcacgccgg cgccatcgac
      241 gagttccgcg atccggactg gtacacgcta tggggcctcg tcgccgagcc cgcgcagcgt
      301 cggctgagct tggccccagg ccagccatgc cgcacgccgt gggtcgagtt gccggttggc
      361 agggacgtgg agggcaaccc cggatgccac tgacaggtcg gcacgtgctc atcaccagtg
      421 gcccgaccca ggcccccatc gacgcggtgc ggtacatcgc gaacacgtcc agcggcgcgc
      481 tcggggcaca caccgccgac gccgctctgg acgccggggc gagcgtgctc ctggtgcacg
      541 gccccgggag catcgtgccc cagccgcacc cacgcctcac cctctcaccc gtgcggaccg
      601 tcgatgacct gatcgccaca ctgcagcgac agctcgccgc gacgcgtttc gacgcgatcg
      661 cccacgccat ggcggtgctc gactatgtcc ccgaggctgc gggaacgggc aagatcccgt
      721 cgggccaaga ccacatcgac ctccgcctgg ttcctacgcc caaggtgatc gaccgcgtcc
      781 gcgagtgggc tccggactcc ctgctgatcg ggttcaaact ggtggcgggt gagagtgacg
      841 agggactgcg ccaggccgcc ctcactctcc ttcgccgcag cggggccgat ctcgtggtcg
      901 cgaacgacat ggtgcagatc caggccggcg agcatcgggc agtgttcatc gaacccagcg
      961 gacgcaccgc gggcgccttc gtcggcaaag ccgcgattgc cgaggccctg gtccgggaga
     1021 tcgctgcccg actggcgggc cgagagggga gtgggctatc gtgagtcatc tcgagggccg
     1081 atcggtcctg gtgggcatca ccggaggaat tgcgtgctac aaggccgccg aactcgtgag
     1141 gtcgctgaga aaggcccaag ctcaagtgcg cgtgatgatg acccgcgcgg cgtgcgagtt
     1201 cgtgacgccc ctgacgttcc agacgctctc gcagaagccg gtggcgaccg acctgttcgc
     1261 accagtctcg gactgggaga tcgagcacat ctcgctggcc cgcggggccg acgtggtggt
     1321 cgtcgccccg gccacggcga atgtcatcgg caaggtggcc cacggcatcg ccgacgacct
     1381 ggtcacaacg gtcatcatgg ccactcgggc tccggtggta ttctgccccg ccatgaatgt
     1441 ggcgatgtgg gagaacccca tcgtgcaggc gaacatccgg cgcctggccg aactgggcta
     1501 ccgcttcgtc gagcccgctg agggtgacct ggcctgcggc gaggagggca aagggcgctt
     1561 ggccgaggtg gagcggatcg tggaggccgt cgcggtcgca ttgccctgag ccactagcga
     1621 ggagcgcacg gatgctgtcg ccaacggtgc gctgtgccgc actgatcccc atcgtggtgg
     1681 tctgctccga cgcctgggcg gcgccatcgc ttcacgatca ggtcgaggcc gactggcggc
     1741 tccaggacca atcgcgcatg cgccacatcg agcagccggg cctggtgcga ttcgtcgaag
     1801 gcgaggtcca gtggcccggc gcccaaccgt cgcagggcgc ccgggtgccg ttcggcgagg
     1861 cgcctcggct cgatgggctc ctcgacgatg cctgctgggc cgcggccatg gtcgtcgagc
     1921 ccgccgcgcc caatcttccg cggttccggc tggcccacga tggcgaacgc ctctacatcg
     1981 gggccgaaat gccgtcgagc accgaggctt ggtaccgggg cgagcccacg ggtatggacg
     2041 cgggcggcgc ggtagatggc gtgcgcgacg ggcgctacgg cttccacacg gggggtgata
     2101 ccgacccctg gtggcaggtc gacctcggcc gaaccacgga gctgggccgc ctcgtggtct
     2161 acaaccggct ggactacgcg ccgggcctgc acaacgccga ccagctccgg gtactcctct
     2221 cagacgacgg gaccgggtgg cgtgaggtct accagaatcc cggtagcttc ttcggcggaa
     2281 tggatgacac cggtccactg cgcgtggacc tgcacgggca acgggcacgt tacgtgaggc
     2341 tgcaggtgcc gggtgccgtg ctgttccatc tcgacgaggt cgaagtgtac ggccccgacg
     2401 gcatcggccc caatctcgcc ctgggccagc cggcccagca gagcagcctg agcatctggt
     2461 cgcgcggcgg tccgtccggc ggcgtgctgg ttcagctggg tgggacgacc gtggtgctgc
     2521 catccggcca gccgccgcag gcggtgaccg acgggcaggc gaagcagacc ggcgccgccg
     2581 ctcgcggggg cggcacgacc cgcctcgaac tcgcactgcc cctcacccag gtcggtgccg
     2641 cctaccccgg agccgcgcgt ctcgccagcg ccgaggcgat cccactgcca ctgggcgccc
     2701 gcttcgacgc tcgccttccc gacgcccccg tgctcggcta tgggatcatc cgcgtccccg
     2761 tggccgtcgg cacagtccgg ggcccttggc ccgggcccgt ggaactatcg gcggagctca
     2821 tcgtgttcac cccgcggcgc atggagcgcg ttccgggccg cgtggtgacc attgatcggc
     2881 cgggaacgac ggaagtgacc gtcaccgcgc gacatgaggg gccggcc
//
LOCUS       DAOSPL010000473         2919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_34046_length_2919_cov_3.162709, whole genome shotgun sequence.
ACCESSION   DAOSPL010000473 DAOSPL010000000
VERSION     DAOSPL010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34046_length_2919_cov_3.162709"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PLQ54_09475"
     CDS             complement(<1..730)
                     /locus_tag="PLQ54_09475"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS93530.1"
                     /translation="MTPRDLSERLECFAEPHRERLRGLLGAPAWQSAIAEGLLDDAAA
                     GGVAPPETENVFALPVEQLVAANGAATRARIAAGERDPERLLDVLGTWPAEWPAELPV
                     RLSFLGLNLGFACNMQPRCVYCNQRPVPSRLRPDRLPELIADARGQVGKGPYVYLTGG
                     EPLLAGPALWGPEGLIRRAAAVGASCNLNTNALALTPEVALGLVRSGLGRAHISVDTH
                     VPEVADGVYGAPGRWQQAMTGLWNL"
     gene            complement(854..1561)
                     /locus_tag="PLQ54_09480"
     CDS             complement(854..1561)
                     /locus_tag="PLQ54_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93531.1"
                     /translation="MLDAAMAKRLAGMLSRFGEYATDPARHGFDDATPEGRAYAARQL
                     NAIREEIAAADPLAAKLFPEIPLEASLEELGTIAGQMLDFISPADASQPRGVGSGIVN
                     SPITINLEGGALAGIKDIGELVRNAVASAMGTVSSALEGLGAKAEEADRKAGPEAEPC
                     APGTEDGAAHDCGPAVRNGVTLALPGFKDQAESIARRMAEIAALGPGMTQEHRDEMAD
                     LSRQLAELVVRAHMPNE"
     gene            complement(1574..2164)
                     /locus_tag="PLQ54_09485"
     CDS             complement(1574..2164)
                     /locus_tag="PLQ54_09485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS93532.1"
                     /translation="MHRTNGRVADRDLVIAAMLGDLACFDELARRYRAAVLATARFEL
                     DDPDLAADVAQEALLLAYRKLPALSAPDAFGPWLRVLTLRLARRARRGWRRIAARYAP
                     LEAAVLLACPAVRPDGAIERSEVQQAVRTAVVALGEPDATLVTLRYLEGMPLERAGAY
                     VGLTRDGAKWRLKRATARLRQSLQHLESDPWDERHS"
     gene            2283..>2919
                     /locus_tag="PLQ54_09490"
     CDS             2283..>2919
                     /locus_tag="PLQ54_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOS93533.1"
                     /translation="MGEGVALTDLTMHKTALTKRDGRYITYYDFAPPGQVPPARTEAD
                     SLTGDPLTKGPELRWNPLLGEWTIVAGYRMDRPQLPRQDKCPLCPGGAEIPSSFEIVS
                     FDNRFPGFVRGAEKPDMPSTELYPVEAGRGVCEVLVYTEAHTGSFAELPTEVIYRLVH
                     VWTDRTRDLSGVPEVRCVYPFENKGREIGVTLDHPHGQIYAFPYIPPRIERE"
BASE COUNT          444 a         1082 c          972 g          421 t
ORIGIN      
        1 ggaggttcca cagaccggtc atagcctgct gccagcggcc cggcgccccg tacacgccat
       61 cggcgacctc gggcacgtgc gtgtcgaccg agatgtgcgc gcgccccagg ccggaacgga
      121 ccaggcccaa cgccacctcg ggtgtcagcg caagcgcatt cgtgttcagg ttgcagcttg
      181 ccccgacagc cgccgcgcgg cggatcagcc cctcgggccc ccagagcgcc ggccccgcca
      241 ggaggggctc gcctccggtc aggtacacgt acggcccctt gccgacttgg ccccgcgcat
      301 cggcgatcag ttcgggcagc cgatccggcc gcaaccgcga aggcaccggg cgctggttgc
      361 agtacacaca gcgcggctgc atgttgcacg cgaatcccag gttcaggccg agaaaggaca
      421 gccgcacggg cagctccgcc ggccactcgg ccggccacgt gcccagcacg tccagcaagc
      481 gttccgggtc gcgctcgccc gccgcgatcc gtgctcgcgt ggcggcgccg ttggccgcca
      541 cgagctgctc caccggcagg gcgaacacgt tctcggtctc cggtggcgcg acgccaccgg
      601 cggctgcgtc atcgagcagg ccctcggcga tggccgattg ccaagcgggc gcccccagca
      661 gaccccggag acgctcgcga tgaggttcgg cgaagcactc cagacgctca gagaggtcgc
      721 gtggcgtcat ggaacacacc acgcgagtgg gactgggtgc ggagctcgga gcgggcgtat
      781 gggcaccagg tgacctccga cgggcgatgg ccttccgata acccttcgcg cccgccccgc
      841 gcggccctcc gcctcactcg ttcggcatat gagcccgcac gaccaactcg gccagctggc
      901 gcgacaggtc cgccatctcg tcgcggtgct cctgcgtcat gcccgggccc agggcggcga
      961 tctcggccat gcgccgggcg atcgactccg cctggtcctt gaagcccggc agcgccagag
     1021 tcacaccgtt gcgaaccgcc gggccgcagt cgtgtgcggc gccgtcttcg gtgccgggcg
     1081 cacacggttc tgcctccggg ccggctttcc ggtccgcctc ttcggctttg gctcccagcc
     1141 cctcgagcgc cgagctcacg gtccccatgg ccgaagccac cgcgttgcgg accagctcgc
     1201 caatgtcctt gatccccgcg agagcgcccc cctccaggtt gatggtgatc ggcgagttca
     1261 cgataccgct cccgactccc cgaggctggg atgcgtctgc tggcgagatg aagtccagca
     1321 tctggcccgc aatggtgccc agctcctcga gggaagcctc gagtgggatc tcggggaaca
     1381 gcttcgcggc cagcggatcg gctgcggcaa tctcctctcg gatcgcattg agctgccggg
     1441 ccgcgtaggc cctcccctcg ggtgtcgcgt catcgaaccc gtgtcgtgcc gggtcggtgg
     1501 catactcgcc gaaccggctg agcatgccgg cgagtcgctt ggccatggct gcgtcgagca
     1561 tgatggatcc tcctcacgag tgtctctcgt cccacgggtc ggattcgagg tgctggaggc
     1621 tctgacggag ccgggcggtg gcgcgcttga gccgccactt ggcgccgtcg cgcgtgaggc
     1681 cgacataggc cccggctcgc tccagaggca tgccctcgag gtaccggagc gtgaccagcg
     1741 tcgcgtcggg ctcgccgagg gccacaacgg ccgtccggac ggcctgctgc acctcggagc
     1801 gctcgatggc tccgtcgggg cgcaccgccg ggcacgcgag caacacggca gcctccagcg
     1861 gggcgtaccg agccgcaatc cggcgccagc cgcggcgcgc ccgccgggcc agccgaaggg
     1921 tcaacacccg aagccacggg ccgaacgcgt ccggcgccga cagcgccggg agcttgcggt
     1981 aggccagcag tagcgcctcc tgcgccacat cagccgccag atctgggtcg tcgagctcga
     2041 accgggccgt ggccagaacg gcggcgcggt agcgccgggc cagctcgtcg aagcacgcca
     2101 ggtcgcccag catcgccgcg atcaccaggt ctcggtccgc cacgcgaccg ttcgttcggt
     2161 gcatgtccgc gagcctccga ttcgataaga ggcactcgca cggcaagatg ggtggtacac
     2221 ttctcccgcc cccgcgcgcg tttccaacgc cgcgcgtgcg ggcccccaac accggccata
     2281 ccgtgggaga aggtgtcgcg ctgaccgacc tgacgatgca caagaccgcc ctcaccaagc
     2341 gcgacgggcg atacatcacg tactacgact tcgcgccccc cgggcaagta ccccccgcgc
     2401 ggaccgaagc cgatagcctc accggcgatc cgctcaccaa ggggcccgaa ctccgctgga
     2461 accccctgct gggcgagtgg accatcgtcg ccgggtaccg aatggaccgg ccccagctcc
     2521 ccaggcagga caagtgccct ctgtgcccgg gcggagccga gataccatcg tctttcgaga
     2581 tcgtgtcctt tgacaaccgc ttccccgggt tcgtccgggg cgcggagaag cccgacatgc
     2641 catcgaccga gctctatccc gtcgaagccg ggcgtggcgt gtgcgaggtg ctggtctaca
     2701 ccgaggccca caccgggtcc ttcgccgagc tgcccacgga ggtgatctat cggctcgtcc
     2761 acgtctggac cgaccggaca cgcgacctgt cgggggtgcc cgaggtgcgc tgcgtgtacc
     2821 ccttcgagaa caagggtcgc gagatcggcg tgaccctgga tcatccacac ggtcagatct
     2881 acgcgttccc ctacataccg ccccggatcg agcgagagc
//
LOCUS       DAOSPL010000474         2915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_34095_length_2915_cov_4.414336, whole genome shotgun sequence.
ACCESSION   DAOSPL010000474 DAOSPL010000000
VERSION     DAOSPL010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34095_length_2915_cov_4.414336"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..1677)
                     /locus_tag="PLQ54_09495"
                     /pseudo
     CDS             complement(220..1677)
                     /locus_tag="PLQ54_09495"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
     gene            complement(1738..2022)
                     /locus_tag="PLQ54_09500"
     CDS             complement(1738..2022)
                     /locus_tag="PLQ54_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93534.1"
                     /translation="MAPRDVARLNSLATAVKSDVRRLARELNAFADDFRSCVSDYADL
                     SAGADPSVGEALCSQAEVFASRVHSWLSALEQQQEALQQAAARLRSGADN"
     gene            2163..>2915
                     /locus_tag="PLQ54_09505"
     CDS             2163..>2915
                     /locus_tag="PLQ54_09505"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOS93535.1"
                     /translation="MDRRAFIEAVGGLAAGSLLAADAGAQEASPMTNALPTNVQMQFM
                     RPEQLEAAGRAFPAVYVPLGLIEWHGRHLPLGNDAIKSHGVLVKCAERHGGVVYPPVY
                     FHDGFDRTHLVPVLTDLFQRLRATGYRVILAVSGHNVQGQIAMANEALAPVVADGTVA
                     GMGLWEVTLSMSADSATDHAAKWETSNMMFLYPDLVDMAALGDGPLALDMKAPDGIGG
                     LDPRVHASAEVGRRNVELAADAIGRKAHELLQS"
BASE COUNT          448 a         1048 c          992 g          427 t
ORIGIN      
        1 ggctggcgcg gcaggctgca ccgagtgcgc cgacggggcg cgcaagtgct gcgatgcctg
       61 cggtgcctgc gccaagagcg acgcgccggt ttcgtaggct agcggttggc acgttgctcg
      121 agacctgaga ccggcccatc gcggggggga gcccacacgg gctctccccc gtaccgcctg
      181 acaggccatg tgggcacggc cggggcctga ccggcggatc tatgcgggcc gtgacacccc
      241 cagcgactgc cgccacgcat cttcggccac ctgctcgagc gcccgcgtgt gttccgggct
      301 cacgtgggtg ggctgatact ggtcgcgcac ctcgcggtac tgctcccacg cccgcgccag
      361 caggcgcgcc tcgcctcggg cctcgcgcgt gtcgtcctgc cagcacccgc ggtccagcag
      421 atgaggcgag tatagcccgc gccagtgctc cagcgtgtgg tccgacgtca tgtggctctc
      481 gccgatccca tcggtgatgt cgaggatcga ctccagcgcc agcgcctcgt cggacactcc
      541 gaccccgcca gcaaagcgcc aaaggtactc gccgaactcc tgatcgagca tgagctgcac
      601 gggcgagaac gtcttgcccg actcgagcaa gcctgagccc gagtgcgggc catcgcccgt
      661 gtggtagtag atggtcaggg cctcgaacgc ctgctcgaag gctcgctgcg atccgggcca
      721 cttggcgtcc gtgtagctgg cgcagcccgc cacatggcac cggcccccgg cgcgatggcg
      781 gaacacctcg acgcacagca ggtcctgcag catggcctcg ggagcgcagt agctcacatc
      841 ggccgtgcgc atatcgagca cgccgctaca aacaccggcc ccgggcaacg cttccggcgc
      901 gaggacgtgg cacgcgaccc accccgccag gatctctgcc acggccagca caacggtccc
      961 cgccacggtg accggcgacg acgccccgga ggccggctgc gttccgatcc acacgctcgc
     1021 gccgcgcggc atgagagcct ggagcagctc gcaagccctg cggtcgaggc gcagcggtgt
     1081 cacggcgaac acgcagatgc cgatgaaccg ctgcgcgtcg ccggtgagga tgcggcccat
     1141 ctctgccagg taaggcacct ggtcggcgta gtgcacatag ccggctgcca cgcgatcggt
     1201 gtgctgcgcc agtgtgatga ccgcctcgat gggctccacc ggtgggggaa cgtcccgcgc
     1261 cagcagcacg gggccgacag gctcgggatc ctgccacacg tggccgaagc gcgtcacctc
     1321 ggccagcagg gcgcggtctg ccggcaggcg ccggcttgtc tccgggtcga ggtagaactg
     1381 cgcgagctgc gatccgatgc cgacgcggta gggcccctgg cgccgggcca gtgaatcggg
     1441 gccgagggcc ggcgaggcca gccggggctc gagcatcgtc tgcaccatgg cccgcggaat
     1501 gcggacacgg tcgcccggtc cgtccaggca gcccgcgacc acgagcgtat ctcgcagagc
     1561 cgggctttgg tacaggaacc cggccgtctc gaggaccttc aaggcaccgt cgaagatgcg
     1621 gtcgagttcc gcgtcggtgg cgagtcgcgc aagggccacg ggcgatccct ccgttggggc
     1681 atacctcgtg ccccgggtta ggcggcgcgg cggcgatctg ccacgacggc gcgccgctca
     1741 gttgtccgcc cccgaccgca gtcgcgctgc ggcctgttgc agggcctcct gctgctgttc
     1801 gagcgctgac agccatgagt gcacccggct ggcgaacacc tcagcctggc tgcacagggc
     1861 ctcgcccaca ctgggatcgg ctcccgccga cagatcggcg tagtcactca cacacgagcg
     1921 gaaatcatcg gcgaaggcgt tcagttcccg cgctagccgc cgcacatcac tcttcactgc
     1981 cgtggccagc gagttgagcc gggccacatc cctcggtgcc actcgcgatc gtcctctcag
     2041 gtgccgttcg gctgctctgc gccggccatg atacagcacc ggctcgccgg gcgaggtatg
     2101 tggtactcgg gccggcgaac gtagcgaggc ccgcaaccgt cgggcgagag ggaggtggcc
     2161 tgatggaccg gcgcgcattc atcgaggcgg tcggcggcct cgctgccgga tcactgctcg
     2221 ctgccgacgc cggcgcccag gaggcttcgc cgatgaccaa cgcgctcccg acgaacgtgc
     2281 agatgcagtt catgcggccc gagcagctcg aggccgcggg gcgcgctttt cccgccgtct
     2341 acgtgcccct gggactgatc gagtggcacg gacgccactt gcccctcggc aacgatgcca
     2401 tcaagtccca cggcgtcctc gtcaagtgcg ccgaaagaca cggcggcgtg gtctaccctc
     2461 cggtctactt ccacgacggt ttcgaccgca cgcacctcgt gccggtcctc accgacctgt
     2521 tccagcggct gagggcaacg ggctatcgcg tcatcctcgc cgtctccggg cacaacgtgc
     2581 aggggcagat cgccatggcc aacgaggccc tggcgcccgt cgtcgccgac ggcaccgtcg
     2641 ccggcatggg gttgtgggag gtcacactca gcatgtcagc cgattccgcc accgaccacg
     2701 ccgccaagtg ggagacatcg aacatgatgt tcctctaccc cgatctggtg gacatggcgg
     2761 cgctgggcga cgggcctttg gccctggaca tgaaggcacc cgacggcatc ggcgggctcg
     2821 atccccgcgt ccacgcctcg gccgaggtgg ggcgcaggaa cgtcgagctc gcggccgacg
     2881 ccattggccg gaaggctcac gagctactcc aatcg
//
LOCUS       DAOSPL010000475         2914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_34110_length_2914_cov_3.465198, whole genome shotgun sequence.
ACCESSION   DAOSPL010000475 DAOSPL010000000
VERSION     DAOSPL010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34110_length_2914_cov_3.465198"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="PLQ54_09510"
     CDS             complement(<1..1126)
                     /locus_tag="PLQ54_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93536.1"
                     /translation="MAMRCGWAACAAALAGLWTAHAADAAPRVEFWVSPDGSDAGPGT
                     RVQPFRTVERARDAIRAARSVAGPATVVLKAGTYRLAQPLHLGPEDSGAAGSPVVYRA
                     ERPGTAILSGGRPITGWVKQPDGVYHAVAPKANFRQLYVNGVRATRARGGPLPDAEPW
                     GELRATIKPLRDGAMYAPLTDVAEAGFRTSNGAMAKWRNPSDIELGFFNYWSHMICRV
                     ESIRPDGDGAILSMAQPGFLFACRKGGVQAGIPDYVENALELLDEPGEWYLDRSEGVV
                     YYKPRPGEDLPTAEVVAPVLTELARVEGTPDAPAHDIRFEGITFSHATWLRPSEYGHP
                     DVQANFVFTADMPVFTRPEFEKGYVTIHSELAKSPANVVVH"
     gene            1223..2077
                     /locus_tag="PLQ54_09515"
     CDS             1223..2077
                     /locus_tag="PLQ54_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOS93537.1"
                     /translation="MKLTVDAARCNLCGACVTACPTDMVRAKPDRIKIGHVACIECGH
                     CMAICPKGAIIDEEGAQLAPVQARPSPEALDALIRSRRTTRRFRSEPVDPSLLGELID
                     AAQYAPSAANCQAQQYVVVSTPDALRHLRERIETYYRTFAAALADRQARDARLARLGL
                     DVEAATHPHVLAAVPAFVKAVDAGRDRLFFDAPAVVVIHADPTEVMPAAACAFAAFSI
                     VLMAEAHGLGSCITGYASDALRMLPDVRHWLGMPETNEVHYVLAVGHPAERYHGIPPR
                     RAAFVRWM"
     gene            2277..>2914
                     /locus_tag="PLQ54_09520"
     CDS             2277..>2914
                     /locus_tag="PLQ54_09520"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOS93538.1"
                     /translation="MQLPEPGEQPLVRCGAIGTNLYGPGTAGGPVEALNTRDPERVLT
                     LYQAYVAAGARALVTNTYAANSLCLSEHELTGECAVINRAGVQIARDAAGVDCAVWGC
                     VGPLSLGFRADDYPAEDLVRIYSEQCEYLSAADALVLETFVDLREARAALQAARATGL
                     PVILQVGRLTAGAARHERLRRLVEEAEACGAVALGANCQHPAETQRTLRELA"
BASE COUNT          452 a         1011 c          993 g          458 t
ORIGIN      
        1 cgtgcacgac gacgttcgcc gggctcttcg ccagctcgct gtggatcgtc acataccctt
       61 tctcgaactc gggccgcgtg aagaccggca tgtcggcggt gaagacgaag ttcgcctgta
      121 catccgggtg gccgtactcg ctcggccgga gccatgtggc gtggctgaac gtgatgccct
      181 caaaccggat gtcgtgagcg ggcgcgtccg gcgtgccctc aacccgcgcc aactcagtca
      241 gcacgggcgc gacgacttcg gccgtcggca ggtcctcgcc cggtcgcggt ttgtagtaga
      301 caactccctc ggatcggtcg agataccact cgccgggctc atcgagcagc tcgagtgcgt
      361 tctcgacgta atcgggaatg ccggcctgca caccgccctt gcggcaagcg aaaaggaacc
      421 ctggctgcgc catcgagaga atggcgccgt cgccatcggg ccggatgctc tcgacccggc
      481 agatcatgtg gctccagtag ttgaagaagc cgagctcgat gtccgacggg ttccgccact
      541 tcgccatggc gccgttggag gtgcggaagc cggcctcagc cacatccgtg agtggggcat
      601 acatggcgcc atcgcggagt ggcttgatcg tagcgcggag ctcgccccac ggctcagcat
      661 cgggcagcgg ccccccgcgc gcccgggtgg cgcgcacgcc gttcacgtag agctgccgga
      721 agttcgcctt gggcgcgact gcgtggtaga cgccatccgg ctgcttcacc cacccggtaa
      781 tgggacggcc accggagagg atcgcggtgc ccggccgctc ggcgcggtag accacgggac
      841 tgccggccgc gccggaatcc tccggcccga ggtggagcgg ctgagcgagc cggtaggtgc
      901 cggccttgag caccacggtg gctggaccgg ccacggacct cgcggcgcgg atagcgtcgc
      961 gcgcgcgctc gaccgtccgg aacggctgaa cccgcgtgcc cggtccggca tcggagccgt
     1021 caggcgagac ccagaactcg acacgcggcg ccgcgtcagc cgcatgggcc gtccacagcc
     1081 ccgccagcgc cgccgcgcag gcggcccacc cacagcgcat tgccacgaaa gtcccccctc
     1141 gtggggcagc cagccaggtc tattgccggg catgcccgaa cccctcggtc atgcacattc
     1201 ggtatgagtg gaggggccac ctgtgaagct cacggtcgat gcggcccggt gtaacctgtg
     1261 cggggcctgt gtcacggcct gcccaaccga catggttcgc gccaagcccg atcggatcaa
     1321 gatcggccac gtggcgtgca tcgagtgcgg ccactgtatg gcgatctgcc ccaaaggagc
     1381 catcatcgac gaagaggggg cccagcttgc gccggtccag gcgcgcccgt cacccgaggc
     1441 cctcgatgcg ctgatccggt cgcgacgcac tacccggagg ttccgatcgg agcccgttga
     1501 cccgtccctg ctcggcgaac tgatcgacgc ggcgcagtac gccccctccg ccgcgaactg
     1561 ccaggctcag cagtacgtgg tggtgagcac gccggacgcc ctccgccatc tccgtgaacg
     1621 aatcgagacc tactaccgga ccttcgcggc cgcgctggct gaccggcaag cgcgcgacgc
     1681 tcgactcgcc cgcctggggc ttgatgttga ggcggccacc cacccccacg tgttggccgc
     1741 cgtccctgcg tttgtcaaag ccgtagacgc tggccgtgat cgactgttct tcgacgcccc
     1801 ggcggtcgtg gtgatccatg cggatcccac cgaggtcatg cccgcggcgg cgtgcgcctt
     1861 cgccgcgttc agcatcgtgc tcatggccga ggcgcatggc ctcggttcct gcatcaccgg
     1921 ctacgcctcc gacgcgctgc ggatgctgcc cgatgtccgc cactggctcg gaatgcccga
     1981 gaccaacgag gtgcactacg ttctggccgt gggacacccg gctgaacgtt accacggcat
     2041 acccccgcgg agagctgcgt ttgtgaggtg gatgtagggg taccaggtgc gcggctcccg
     2101 ttggccctga gacgcgtgtg gtggatggcg gtggctgcac gcggcgcggg tcgccgcggc
     2161 tcaggaacgg cagtttcggg ggctcggcgg cgtcatggcg actacgggcc gcatggaggc
     2221 agatgacggc gcgcatgcta tgatagcccc actcacctgc ggagaggtac accgacatgc
     2281 agctccccga acctggcgaa cagcccctgg tgcgttgcgg ggccatcggg accaacctat
     2341 acggcccggg aacggccggc ggaccagtgg aggccctcaa cacccgcgat cccgaacggg
     2401 tgctcacatt gtaccaggcc tacgtggccg cgggcgcccg ggccctcgta accaacacct
     2461 atgccgcgaa ctcgctttgc ttgagtgagc atgagctcac cggcgaatgt gctgtgatca
     2521 accgagccgg cgtccagatt gcacgagacg ctgccggggt ggactgtgcc gtctggggat
     2581 gcgtgggacc actctcgctc ggtttccgcg ccgacgacta ccccgccgag gacttggtcc
     2641 ggatctactc cgagcagtgt gagtacctgt ccgcggccga cgccctggtg ctggagacct
     2701 tcgttgatct gcgcgaggcc cgagcggccc tgcaagcggc tcgcgcgacg gggctaccgg
     2761 tgatcttgca ggtcggacgg ctcacggcgg gcgcggcgcg ccacgagcgc ctgcgacggc
     2821 tggtcgagga ggccgaagcg tgcggtgctg tggccttggg cgccaactgc cagcatcctg
     2881 ccgagaccca gcgcacactc cgcgagctgg ctca
//
LOCUS       DAOSPL010000476         2914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_34112_length_2914_cov_3.329836, whole genome shotgun sequence.
ACCESSION   DAOSPL010000476 DAOSPL010000000
VERSION     DAOSPL010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34112_length_2914_cov_3.329836"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /locus_tag="PLQ54_09525"
     CDS             complement(<1..1385)
                     /locus_tag="PLQ54_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOS93539.1"
                     /translation="MFGEEAGRGAIIGVVLVICAVGFLGFLASRWRKAGPNEALVVYG
                     LRKKCVVGGGVFVIPGFETCHSLSLEIMTLDVTTPEVYTAEGVPVIVDGVAQVKVDGS
                     DEAIRTAAEQFLGKSTTETMSIALQTVEGHLRAILGTMTVEDIYKNRDAFAQQVQEVA
                     ASDLANMGLRIVSFTIRDIRDNKGYLEALGKPRIAQVKRDATIGEAEAQRDATIRSAQ
                     ANQVGQEARYQAEANIAQAERDYNIKRPEYNQAVQLKQAEADLAYELQKNRTNQEIRR
                     EQVQVDVVEKEMQIKVQDAEIERRQRELAATVQRPADAERYRLETIAQGEKSRIEIEA
                     EAKGNAARAIGVGEAEAAKAKGIAMAEVRKATGLAEAEVAKAVGLAEAEAIRAKGSGE
                     AEAMAKKAEAWKQYNEAAIVEKVVSVLPDIASAIATPLAKTERIVMVNTGGDGAGGVG
                     VSRITGDIATV"
     gene            complement(1527..2219)
                     /locus_tag="PLQ54_09530"
     CDS             complement(1527..2219)
                     /locus_tag="PLQ54_09530"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOS93540.1"
                     /translation="MGTALTIYWASFVFGALFLLLLGVLGHFGGHDAGGHDIGGHDVG
                     GHDIGGHDVGGHDAGGHGAAGHADGGGAHGPASPSSQFSGISPLSPLIISCFLTIFGA
                     TGIACASIGVPPLISAPLASVSALVISALAFVAFSRLLMRIQSTSHYALHELEGLEGT
                     VVTPIPASGVGEVAVVAHETRRTNPARSADGSAIEKHVTVVVERVVGNTLMVRKAIDE
                     RLRELRPEGPSA"
     gene            complement(2256..>2914)
                     /locus_tag="PLQ54_09535"
     CDS             complement(2256..>2914)
                     /locus_tag="PLQ54_09535"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOS93541.1"
                     /translation="SRAEDPEKQLEALIRRMEANLQEARAEIAAAARDERRLNEDLTD
                     CRQQGDEWAQKARLAVEQSKDDLAREAIRRRLACERRAQHVTDELALQRAGVQTLRDH
                     LAALEAKVEETRRYRDLIVAHRRLAIAQNGMARRAGVRASEHLDRIEDRVIEIEATAV
                     AAQDELDTRLSVIEQRARSGEEDQAVERELAALKSEMAGRVDPADPPRKVSQRRKPTP
                     "
BASE COUNT          427 a         1021 c          926 g          540 t
ORIGIN      
        1 atgaccgtgg cgatgtcgcc ggtgatgcgt gagacaccca ccccgcccgc gccgtctccg
       61 cccgtgttga ccatcacgat gcgttcggtc ttggcgagcg gcgtggcgat ggcgctggcg
      121 atgtcgggca gcaccgacac gaccttttcg acgatcgctg cctcattgta ctgcttccaa
      181 gcctccgcct tcttggccat ggcctcggcc tcacccgagc ctttggcccg gatggcttcg
      241 gcttcggcga ggcctaccgc tttggcgacc tccgcctcgg ccaagccggt tgccttccgc
      301 acctcggcca tggcgatgcc tttggctttg gccgcctctg cctcgcccac gccgatagcg
      361 cgtgcggcgt tccccttggc ctccgcctcg atctcgatcc gggacttctc gccctgagcg
      421 atggtctcga gccggtatcg ttcggcgtcc gctggacgct gaaccgtggc ggccaactcg
      481 cgctgccgcc gctcgatctc ggcgtcctgc accttgatct gcatttcctt ctcgaccaca
      541 tcgacctgca cttgctcgcg acgaatctcc tggttggtcc tgttcttttg cagctcgtag
      601 gccaggtcgg cctcggcctg cttgagttgg acggcctggt tgtactcggg ccgcttgata
      661 ttgtagtccc gctcggcctg ggcgatgttg gcctctgcct ggtagcgggc ttcctgccca
      721 acctggtttg cctgcgccga ccggatcgtg gcgtctcgct gcgcttcggc ctcgccgatg
      781 gtggcgtcgc gtttcacctg ggcgattcgg ggcttgccca gggcctcgag gtagcccttg
      841 ttgtcgcgga tgtcacggat ggtgaaggac acgatccgca agcccatgtt ggccagatcg
      901 ctggctgcca cttcctgcac ctgctgggcg aaggcatcgc ggttcttgta gatgtcttcc
      961 accgtcatgg tgcccaagat ggcgcggagg tggccctcca ccgtctgcag ggcgatcgac
     1021 atggtctcgg tggttgactt gcccagaaac tgctcggccg cagtccgtat cgcctcgtcg
     1081 gacccgtcga ccttcacctg agccacgcca tccacgatga cgggcacccc ctcggccgtg
     1141 tacacctcgg gtgtggtgac atcgagggtc atgatctcca aggagaggga gtggcatgtc
     1201 tcgaagccgg ggatcacgaa gacgccgccg cccacgacgc atttcttacg caagccgtac
     1261 acgaccagcg cctcattggg cccggctttg cgccagcgcg aggccaggaa gcccaggaaa
     1321 ccgacggcac agatgacgag gaccaccccg atgatggccc ctcttcccgc ttcctctccg
     1381 aacatgaccg aacacctccg ttggacagtc gcccgggtcg aggcatgaac cgacccggcg
     1441 tggtgcttcc ggtccggacg cgcgacactc cgctcagtcg cgcggagtgc cggcaggcgg
     1501 ttccgaatgg cgggcgggcg cacctctcag gccgagggcc cctctggccg aagctcgcgg
     1561 agtcgctcgt cgatggcctt gcgcaccatg agcgtgttgc cgaccacacg ttccacgacg
     1621 accgtgacat gcttctcgat ggccgaaccg tcggctgagc gcgcgggatt cgtccgacgg
     1681 gtctcgtgag ccaccacggc gacctcaccc acaccgctgg cggggatggg agtcaccacg
     1741 gtcccctcca gtccctcgag ctcgtgcagc gcatagtgac tggtgctctg gattcgcatg
     1801 agcagccgac tgaacgcgac gaacgccagg gccgagatga ccagggcgga gaccgacgcc
     1861 agcggagcgc tgatcagcgg cggcacaccg atgctcgcac aggcgatgcc agtggcgccg
     1921 aagatcgtca ggaagcacga gatgatcagc gggctaagcg gtgaaatacc tgagaactgg
     1981 gaactcggac tcgcagggcc gtgcgcgccc ccgccatcgg catggccagc tgcaccatgg
     2041 ccgcccgcgt cgtgaccacc aacgtcgtgg ccgccgatgt cgtggccacc aacgtcgtgg
     2101 ccgccgatgt catggccgcc cgcatcatga ccaccgaagt ggccgaggac acccagcaga
     2161 agcaagaaca gtgctccgaa cacgaacgac gcccagtaga tcgtcaacgc cgtacccatg
     2221 gcatacttcg cctccatcgc gcccggcacc gcaggctatg gcgtgggctt ccgcctctgg
     2281 gacaccttcc gggggggatc cgcgggatcg acgcgacccg ccatctcgga tttcagcgcc
     2341 gccagctcgc gttcgacggc ttggtcttcc tcgccactcc gggcccgctg ctcgatcacg
     2401 gagagccgcg tgtccagctc gtcctgggcg gcaaccgccg tggcttcgat ctcgatgacc
     2461 cggtcttcga tgcggtccag gtgttccgag gcccgaacgc ccgcccgccg cgccatgccg
     2521 ttctgggcga tggccagtcg gcggtgggcg acgatgaggt cacggtatcg ccgcgtttcc
     2581 tccaccttgg cctcgagggc ggccaggtga tcgcgaagcg tctgcacacc ggcgcgctgg
     2641 agggcaagct cgtcggtcac gtgctgggcg cggcgttcgc aggccaatct ccgacggata
     2701 gcctcccgag ccaggtcatc cttgctttgc tcgacagcga ggcgcgcctt ttgggcccat
     2761 tcgtctccct gctgccggca gtcagtgagg tcctcgttga ggcgacgctc atcgcgagcc
     2821 gccgccgcga tctcggcccg ggcctcctgg aggttggcct ccatccggcg gatcagggcc
     2881 tcaagctgct tctcggggtc ctccgcccgg ctga
//
LOCUS       DAOSPL010000477         2911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_34166_length_2911_cov_3.078081, whole genome shotgun sequence.
ACCESSION   DAOSPL010000477 DAOSPL010000000
VERSION     DAOSPL010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34166_length_2911_cov_3.078081"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PLQ54_09540"
     CDS             complement(<1..784)
                     /locus_tag="PLQ54_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93542.1"
                     /translation="MPAVPGVGGHAHDEELAPAAAVAPLRPGAVRRARFLAQAISLCC
                     DFGAAAAIAYALVLLEGSTVPRYGGIPVHLLAAGVFVGFAWLGRAYEARLIASGYGVL
                     RGVLVAAAASLGLCGWLIGAGLGSSIGMILFLSLAALLVREACARLLGAHALADRVII
                     LGIEELSAAMARTLPGVVGPTHHVIGFIDDRTDPRRYPRGTGAVLGRIADASRIAREY
                     QADVVVVGIAGALRPAITEALSQCIEEGMDIVRIADFYTGCFQ"
     gene            complement(1025..1840)
                     /locus_tag="PLQ54_09545"
     CDS             complement(1025..1840)
                     /locus_tag="PLQ54_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93543.1"
                     /translation="MNMTSRDRNILLGFSLVVVVGAAVAIAVRTSGGSGDSGPGTKAL
                     PAPHAPEGARRAADASGQTAELARNVFRPLIAGLPSDRALSAPGPSSAVGAKSQSTAT
                     KSPGRGNWRSGTSKGPDGGTGGGEPKGEPIKIALVGITNGPNGPRALVENEKRESRYV
                     ALNEDAFGYTLSYLAQTGAIFSQNDRYYVLKLGEGKQVAEAPAPAAPSQTSDASKTGA
                     PSSAGGLPPTMSFDASMMSGPSPEMMMKWQSSGGEGRRGRRGGRNGAAVKVGF"
     gene            2053..>2911
                     /locus_tag="PLQ54_09550"
     CDS             2053..>2911
                     /locus_tag="PLQ54_09550"
                     /inference="COORDINATES: protein motif:HMM:NF018123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HOS93544.1"
                     /translation="MDANPFLGVFLHAIGGLAAGSFYIPFRRVKGWVWECYWLAGGVF
                     SWIIAPWVAAWIIVPDPIGVLRDAPVGALAASFGFGVLWGIGGLTFGLSMRYLGMSLG
                     YALALGMCAAFGFIIPPLASGELTHMVATPSGVVILAGVAVCLLGIAVCGQAGARKER
                     ELPDEQKKVAIREFNFAKGVWVALFAGVMSACMALGIAAGKPIGDLAVERGTPDLWRN
                     IPVWIVIMAGGFVTNCVWCVFLSVKNKTAGDYLKGGASLLPNYLFSALAGITWYCQFF
                     FYGMGTTQMG"
BASE COUNT          474 a          970 c          997 g          470 t
ORIGIN      
        1 tctggaaaca gcccgtgtag aagtcggcga tgcgaacgat gtccatgccc tcttcgatgc
       61 attgggatag cgcttcggta atggcgggcc gaagcgcgcc ggcgatccca acgaccacaa
      121 cgtccgcctg gtactcgcgg gcgatccgcg atgcatcggc aatccgtccc agaacagcac
      181 ccgtgccccg cgggtagcgg cggggatcgg tgcggtcatc gatgaacccg atgacgtggt
      241 gggtgggccc gacgacgccg ggaagcgtgc gggccatggc agcgctgagc tcctcgatgc
      301 ccaggatgat gacccggtcg gcgagggcgt gggcgcccag aagccgagcg caggcctccc
      361 gtacgagaag cgcggccagg gagagaaaga ggatcatgcc gatggagctt cccaagcccg
      421 cgccgatcag ccagccgcac aaccccaggc tggcggcggc cgcgacgaga acgcctctca
      481 gcacaccgta cccgctggcg atcagcctgg cctcataggc cctgccgagc caggcgaagc
      541 cgacgaacac acccgctgcc agcaggtgga ccgggatgcc tccgtaccgg ggcaccgtac
      601 tgccctccag caggacgagg gcgtacgcga tggcggcggc ggcaccgaag tcgcaacaca
      661 atgagatggc ttgtgccagg aaccgggcgc ggcgcaccgc gcctggcctc agcggcgcga
      721 cggcggcggc tggcgcgagc tcttcgtcgt gtgcatggcc acccacccca gggaccgcgg
      781 gcatgggtct acccgccgga cgaggtgcgt ccctggccaa taacctgaga gtccctgatc
      841 ccacggcctg actgcccggg ccgtgcccca gcaccgggct ctacctccct gcgtggcggc
      901 gccaaagacc aatcgcacgt tagcccccaa ggctaacgcg ctaccagcaa ggagtattcc
      961 accgagtggc caaacgcaca catgaaatcg cggggaaggc agcgcgccaa ccggccccat
     1021 cgcttcagaa cccgaccttg accgccgcac cattccggcc gccccgacgg ccgcgccggc
     1081 cctcgccgcc cgatgactgc cacttcatca tcatctccgg gctgggtccc gacatcatcg
     1141 aagcgtcaaa ggacatggtt ggcggcaaac cacccgccga cgatggcgcg ccagtcttgg
     1201 aggcgtccga agtctggctc ggtgccgcag gcgccggagc ctcagcaacc tgcttgcctt
     1261 cgcccagctt gagcacatag taccgatcgt tctgcgagaa gatggcaccg gtctgcgcca
     1321 ggtaagagag cgtgtagccg aaggcatcct cgttgagggc aacgtagcgc gactccctct
     1381 tctcgttctc gaccagcgcc cgcgggccgt tgggcccgtt ggtgatcccc accaaggcga
     1441 tcttgatggg ctcgcctttc ggttcgccgc cgcccgttcc gccgtcgggc cccttgctgg
     1501 tgccggaacg ccagttgccg cggccgggcg acttggtcgc ggtgctctga gacttggccc
     1561 ccacggctga ggagggacct ggggcggaga gtgcccgatc ggagggcagg ccggcgatca
     1621 gtggccggaa gacgttgcgt gccagctcgg ccgtctgtcc cgacgcatcg gctgcgcgcc
     1681 gagcgccctc gggcgcgtgc ggggccggca gggctttcgt gcctggtccg gagtcaccgc
     1741 tcccgccgga ggttctcacg gcgatggcca cggcggcgcc caccacaacc accagggaga
     1801 acccgagcag gatgttgcgg tcgcgcgagg tcatgttcat tcgcgaacct cacagggggg
     1861 actgggcaac cacaccatat cgacgaagga cgacgggttc acacccatgt tcactacgcc
     1921 gcccgccgac cgattccttc gtgaggcgcc gtcgttggag acccggcaag gcgaacacgg
     1981 gtcgattcgc gaagagggtc agggccgagc ggatgccggc agatgccgta tggacattga
     2041 ggagcgtgtc agttggacgc caaccctttc cttggagtgt tccttcacgc catcggcggc
     2101 ctggccgcgg gcagtttcta catcccattc cgccgggtca aggggtgggt ctgggagtgc
     2161 tactggctgg cagggggagt cttcagctgg atcatcgcgc cctgggtcgc ggcatggatc
     2221 atcgtgcccg acccgatcgg tgtgctccgc gacgcgcccg ttggcgctct cgccgcgagc
     2281 ttcggcttcg gcgtgctgtg gggcatcggc ggcctgacat tcggcctatc gatgcgctac
     2341 ctcggcatgt cgctcggcta tgcgctggcg ctgggcatgt gtgcggcctt cgggttcatc
     2401 atcccgcccc tggccagtgg cgagctcacc cacatggtgg cgacgccgtc gggggtcgtg
     2461 atcctggccg gcgtggccgt gtgtctgttg gggatcgcgg tgtgcggcca agccggggct
     2521 cggaaggaga gggagctgcc cgacgagcag aagaaggtgg caatccgtga gttcaatttc
     2581 gccaagggcg tctgggtggc cctgttcgcc ggcgtgatga gcgcgtgcat ggcgctcggg
     2641 atcgcggcgg ggaagccgat cggcgacctg gcggtcgagc ggggcacgcc cgacctctgg
     2701 cggaacatcc ccgtatggat cgtgatcatg gcgggtggct tcgtcaccaa ctgcgtgtgg
     2761 tgcgtgtttc tcagcgtcaa gaacaagacg gcgggtgact acctcaaggg cggggcttcc
     2821 ctgctcccca actacctgtt ctccgccttg gccggcatca cgtggtactg ccagttcttc
     2881 ttctacggta tgggcaccac ccagatggga a
//
LOCUS       DAOSPL010000478         2910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_34183_length_2910_cov_3.644834, whole genome shotgun sequence.
ACCESSION   DAOSPL010000478 DAOSPL010000000
VERSION     DAOSPL010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34183_length_2910_cov_3.644834"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PLQ54_09555"
     CDS             <1..214
                     /locus_tag="PLQ54_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93545.1"
                     /translation="YLDPDDPIQVGSDWITIVAATAGAKTIRLPRAKPVLDIAAGRRI
                     ARRTAEFTVDMAAGETRIYYLGPETR"
     gene            244..>2910
                     /locus_tag="PLQ54_09560"
     CDS             244..>2910
                     /locus_tag="PLQ54_09560"
                     /inference="COORDINATES: protein motif:HMM:NF022752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOS93546.1"
                     /translation="MMVALATPSAPGSPRATVHESGDGCWVIENRLIAVTLDAHAGTL
                     SVRDARNGWVWRQAHGSSRGFRDVQPSAGARAGLRFVAEDAARPPVRVETTVPDDAAE
                     VHIQVSLDDPERPVDSFAPMAPFVLEERPGFVIGAQYGDGVLIETDDLTWSGSYWTTY
                     RGLDMPWVGLTDLRKGYALIVETPFDAAIRLDQAPVGDAKVLAPTVVWEPCKGRFAYP
                     RRCIYSFHAEGGHVALAKRYRHYAEAQGLVRTLREKAEANPEIDKLLGAPDIWGADGL
                     AFCREAKLLGVDRALVNGRWSPDDMKAMIDLGYLVSEYDNYVDILPLAEGAEPDSNHA
                     PIPGHCLKYADGSPVVGWITYDKSQTYMKRCSSLAPAAAKNVVDRVLAQFPYNARFLD
                     VSTADTLFECYDAEHPMTREDDARHRMEMAQVLRDRGLVVGGEHGIFWAVPNYEYFEG
                     MMSGGHASWPAGHLMKPESLAGISDEYRKYGIGHAHRAPLWELVFHDCAVSTWYWGDA
                     TGWLYELDPTIVDKQDALNVLYGTVPLMWAGSSDRYFPDHKERFLQSYRNTCKLHEQI
                     GYDEMLSHEFVTEDRAVQRTRFSSGVTVTVNFGDAPFALDADGQTYPLPTHGFLVTDA
                     SGRVIQFRAESGAGVATLIRAPGYVYAEAPAAGRLDVGGLSVTGSVTCRTPEPTRLLL
                     RGTGSVTLRPGDVAAGWDPATTVAFIRDPDGNRTERLTLAAADGRITAELPAGGDIEL
                     VCGPPAELPDLAIHGEDLRVTPAAVRQGEAVVATLVVRNDGLSLGDGDLICYLDRVDH
                     NHEVAAEPMQLEPGQSRSYEVRLDTASLDGTRHFIARFLTGDTAHDLLLANNTAAIEF
                     TVEPDFGRWSHSIAASVDAGTLARRDVPVALSV"
BASE COUNT          487 a         1018 c          954 g          451 t
ORIGIN      
        1 ctatctcgac ccggacgacc cgatccaggt cggatccgac tggatcacca tcgtcgcggc
       61 cacggccggg gccaagacca tccgtctgcc ccgggccaag cccgtgctcg acatcgctgc
      121 aggacgccgg atcgcgagaa gaacggccga gttcaccgtc gacatggcgg ccggagagac
      181 gcggatctac tacctggggc ctgagacgag gtaggaggcg caagaacgat agttctgctg
      241 ggcatgatgg tggctctggc cacgccttcg gcgccgggaa gccctcgggc tacggtgcac
      301 gagtcgggcg acgggtgctg ggtcatcgag aaccgtctca tcgcggtgac tctcgatgcg
      361 cacgccggca cactctcggt ccgagatgca cggaacggct gggtgtggcg gcaagcccac
      421 ggctcgtctc ggggattccg cgacgtgcag cccagcgccg gggcccgggc gggactgcgg
      481 ttcgtggccg aagacgcggc caggccccca gtgcgggtcg agaccacggt gcccgatgac
      541 gccgccgagg tgcacatcca ggtgtcactg gacgacccgg agcggcccgt cgactcgttc
      601 gcccccatgg caccgttcgt actcgaggag cgcccggggt ttgtcatcgg tgcccagtat
      661 ggcgacggcg tgctcatcga gacagacgac ctgacctggt ccggaagcta ctggacaaca
      721 taccgcggcc tcgacatgcc atgggtcggc ctcaccgacc tgcgcaaggg ctacgccctg
      781 atcgtcgaga cgccattcga cgcggccatc cggctggacc aggcccccgt gggcgatgcc
      841 aaggtcctcg cacccaccgt cgtgtgggaa ccgtgcaagg gccggttcgc gtacccccgg
      901 cgctgcatct actcattcca cgccgaaggt gggcatgtgg ccctcgccaa gcgttaccgc
      961 cactacgcgg aagctcaggg gctcgttcgg accctgcgtg agaaggccga ggccaacccg
     1021 gagatcgaca agctcctggg cgcgcccgac atctggggcg ccgatggcct ggccttctgt
     1081 cgcgaggcca agctcctggg cgtagaccga gcgctggtca acggccgctg gtctcccgat
     1141 gacatgaaag ccatgatcga cctgggctat ctggtgagcg agtatgacaa ctacgtcgac
     1201 attctgcccc tggccgaggg ggccgagccg gattcgaacc acgcacccat ccccgggcac
     1261 tgcctgaagt atgccgatgg ctcgcctgtg gtgggctgga tcacctacga caagagccag
     1321 acctacatga agcgctgctc gtccctggcg ccggcggccg ccaagaacgt cgtcgaccga
     1381 gtcctcgccc agttccccta caacgcgcgg ttcctcgacg tctcgacggc cgacacgctg
     1441 ttcgagtgct acgacgccga acacccgatg acgcgtgaag acgacgcgcg ccaccgcatg
     1501 gagatggcgc aggtcctgcg cgaccgcggc ctggtagtcg gaggcgagca cggcatcttc
     1561 tgggccgtcc ccaactacga gtacttcgag ggcatgatga gcggcgggca tgcgtcatgg
     1621 ccggccgggc acctgatgaa gccggagagc ctcgcgggca tcagcgacga gtaccgcaag
     1681 tacgggatcg gccacgcaca tcgagcgcca ctgtgggagc tcgtgttcca tgactgcgcc
     1741 gtgagcacct ggtactgggg cgatgccacc ggctggctct acgaactcga tcccaccatt
     1801 gttgacaaac aagatgcact caacgtgctc tacggcaccg tccctctcat gtgggccggt
     1861 agcagcgacc ggtacttccc ggaccacaag gaacgcttcc tccagtcgta ccggaacacg
     1921 tgcaagctgc acgagcagat cgggtacgac gagatgctct cgcacgagtt cgtcacggaa
     1981 gaccgcgcgg tgcagcgcac gcggttctcc tccggcgtca cggtgaccgt gaacttcggc
     2041 gatgcgccgt tcgctctcga cgccgatggg cagacctatc cgttgccaac ccacggcttc
     2101 ctggtcaccg atgccagcgg gcgcgtcatc cagttccgag ccgaatccgg cgccggcgtg
     2161 gccacgctta ttcgcgcgcc gggctacgtc tacgccgagg cgcccgccgc tggccggctg
     2221 gacgtgggcg gactctctgt gaccggcagc gtcacgtgcc ggactcccga accaacgcgg
     2281 ctcctgctcc gtgggaccgg ctccgtgacg ctccgtccgg gcgacgtggc ggccggatgg
     2341 gatccggcga ccaccgtcgc cttcatccgc gatcccgacg ggaaccgcac agaacgactc
     2401 acgctcgccg ccgccgatgg ccggatcacg gccgagctgc cggccggagg tgacatcgag
     2461 ctcgtctgcg gcccgccggc tgagctccct gatctcgcga tccatgggga ggacctgcgc
     2521 gtcaccccgg ccgccgtacg ccagggagag gcggtcgtgg cgactctcgt ggttcgtaac
     2581 gacggcctct cgctgggcga cggggacctc atctgctacc tcgaccgggt cgaccacaac
     2641 cacgaggttg ccgccgagcc catgcagctc gagccgggcc agagtcggtc ctatgaagtg
     2701 cgactcgaca ccgcctcgct ggacgggact cggcacttca tcgcccggtt cctcaccggc
     2761 gacaccgccc acgatctgtt gctcgccaac aacacggcgg ccatcgagtt caccgtcgag
     2821 cccgacttcg gccgctggtc gcacagcatc gccgcgtcgg tcgacgcggg cactctcgcg
     2881 cgtcgcgacg tacccgtggc gctgtcggtc
//
LOCUS       DAOSPL010000479         2905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_34258_length_2905_cov_3.341404, whole genome shotgun sequence.
ACCESSION   DAOSPL010000479 DAOSPL010000000
VERSION     DAOSPL010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34258_length_2905_cov_3.341404"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2886
                     /locus_tag="PLQ54_09565"
     CDS             <1..2886
                     /locus_tag="PLQ54_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93547.1"
                     /translation="LSTVHTASLIALWALTASHADAAQVSDGTVSVRFDTQSGRFAIA
                     DDDIGTVLSDGYIVARAGAAEVDTRNAARITAVKERIGPTGPEIMVRYRPGVLLVFGV
                     RGAEGRVEVTASMPDEASIARARARMQMGPEPVIGRLIDERAGDAGVLTTTLGPANIP
                     SARSLFDRRHDVAVTVDAAEGRARLSHELGDPADRYEVTSSARSGELLLGVTVRKHYY
                     RDTLGIAHYAPLSKSSFFRTAPCVAMTWYGLPGWAGRPAQTRERLFPQIDWVARNLRP
                     YAPNLVFQLDDNYAVNDATMRDISDYIRGRGMIPGVWSTPFTAVPAAVYDSHREWFLH
                     DAQGNSIRTFGGIIFGSEPYAGHTLDVGNPQAMEQAFLPWWRRMCDTWGFDFFKIDGQ
                     PHVIDAYRAVHGSAGVEAYRRGLDAARDVVGNARFINGCWGIPLEAIGKVDGSRTGGD
                     TGQWPHAIDVIIRWNFLNNTAWWCDPDAAANLADAEIERVRLNTQARTLTGQQFLTDE
                     VWTRVRPDVAHAWQRGMPNADIKPANLYEVPDWQRYDVIDLKVERPFGRWDVVGVFNY
                     GPDPKSRDLRLAELDLEPGEYHVYDFWAQDYLGTMSDTATMPVHLAAYEGRLFALHRD
                     TGAPQVLSTSRHITQGGIDLAAVSVSRDGDGWRIDGRSEQLVHGDPYALTIVTGPHRV
                     VRAWSDAGDATWAEEGAVTRVTLTPTGTTRAGWAIRLEPLKGPRLRAHPLRVALGSVD
                     PAAPRPTMCLANAGPTSVGWRAWSDSAWVAPSPDRGRLGPYPSQADVELSLTPGLLPR
                     HGTCEATIWIRPEGRSGQPPQAVRVRYTAPPPPNLALGARASSSSDWSAEHAAAKVSD
                     GRADTRWNSAHGDRDGAWIALEWPSAVVCDAVRIREAPDLGPRVQTWVLEGFDGTRWI
                     RLADGGPIGAAHTATFAQRSLLRLRLTIRSASDVPTFTEIEVYDRGG"
BASE COUNT          435 a         1040 c          992 g          438 t
ORIGIN      
        1 ctgagcacgg tacataccgc gtcgctgatc gccctctggg cgctcactgc cagccatgca
       61 gacgccgcgc aggtgtccga tggcaccgtc tccgtgcgat tcgacactca gtccgggcgc
      121 ttcgccatcg ccgacgacga catcggcacc gtcctctccg atgggtatat cgtcgcccgg
      181 gctggcgcgg ccgaggtcga cacacgcaat gccgcccgga tcacggcggt caaggagcgt
      241 atcggcccga ccggacccga aatcatggtg cggtaccggc cgggcgtgct cctcgtgttc
      301 ggcgtgcgcg gggccgaggg ccgcgtcgag gtcaccgcct cgatgccgga cgaagccagc
      361 atcgcgcgcg ccagggcgcg gatgcagatg gggccagaac cagtcatcgg gcgcctcatc
      421 gatgagaggg ccggcgatgc cggcgtgctc accactaccc tgggccccgc caacatcccc
      481 tctgcccggt cgctgttcga ccggcggcac gacgtggccg tcaccgtcga tgcggccgag
      541 ggccgggccc ggctcagcca cgagctggga gacccggccg accgctacga ggtcacctcg
      601 tcggcccggt ccggcgaact cctgctgggt gtcaccgtcc gcaagcacta ctaccgcgat
      661 acgctgggga ttgcccacta cgcgcccctg tccaagtcga gcttcttccg gaccgcgccc
      721 tgcgtcgcga tgacctggta tggcctgccc ggctgggccg ggcgcccggc tcagacacgc
      781 gagaggctgt tcccccagat cgattgggtc gcccggaacc tccgacccta tgctcccaac
      841 ctcgtcttcc agctcgatga caactacgcg gtcaacgatg ccaccatgcg ggacatatcg
      901 gactacatcc gcggccgcgg gatgatcccc ggagtctggt cgacaccctt cacggcggtg
      961 ccggcggcgg tctacgactc ccatcgcgag tggttcctgc acgacgccca ggggaactcc
     1021 atcaggacct tcggcgggat catcttcggc agcgagccct atgcgggcca cacactggat
     1081 gtcggcaacc cgcaggccat ggagcaggcc ttcctcccct ggtggcggcg catgtgtgac
     1141 acctgggggt tcgacttctt caagatcgac ggccagcccc atgtgatcga tgcctaccga
     1201 gcggtgcacg gatccgccgg ggtcgaggcg taccgccggg ggctcgatgc ggcccgggac
     1261 gtcgtgggga acgcgcgttt catcaatggg tgctggggca ttccgctgga ggccattggg
     1321 aaggtggacg gctcgcgaac cggtggcgac accgggcagt ggccgcacgc catcgatgtc
     1381 atcatccgct ggaacttcct caacaacacg gcctggtggt gcgacccgga cgcggccgcc
     1441 aacctggccg atgccgagat cgagcgcgtc cgactcaaca cgcaagcccg gaccctcacg
     1501 gggcagcagt ttctgaccga cgaggtgtgg acgcgcgtcc ggcccgatgt ggcccacgcg
     1561 tggcagcggg gcatgcccaa cgccgacatc aagcccgcca acctctatga ggtgcccgac
     1621 tggcagcggt acgatgtgat cgacctgaag gtcgagcggc cgttcggccg gtgggacgtg
     1681 gtgggcgtgt tcaactacgg gcccgacccc aagtcacgcg acctgcgact ggccgagctg
     1741 gatctcgagc ccggggagta ccacgtgtac gacttctggg cacaggacta cctcggcacg
     1801 atgtccgaca cggccacgat gcccgtgcac ttggccgcct acgaggggcg gctgttcgcg
     1861 ctccaccgcg acaccggggc accgcaggtg ctgtccacct cgcggcacat cacccagggc
     1921 ggcatcgatc tggcggccgt aagtgtctcg cgggatggcg atggctggcg catcgacggg
     1981 cgttcggagc agcttgtgca cggcgacccc tatgccctga ccattgtgac cggtccccat
     2041 cgggtggtgc gggcctggtc ggacgcgggc gatgccacct gggccgagga gggcgcggtg
     2101 acccgcgtca cactgacgcc cacgggcacc actcgggccg ggtgggccat ccggctcgag
     2161 ccgctgaagg gcccccggct gcgcgcgcac ccgcttcgcg ttgcgctcgg atccgtggat
     2221 ccagccgcgc cgcggccgac gatgtgcctc gccaacgcgg ggcccacgtc ggtcgggtgg
     2281 cgagcctggt ccgactccgc ttgggtcgca cccagccccg accggggccg cctcggaccg
     2341 tacccgtcgc aggcggacgt cgagctatct ctcacccctg gtctcctgcc gcgccatggc
     2401 acctgcgagg ccaccatctg gatccgcccc gaaggccggt ccggccaacc cccacaggca
     2461 gtccgggtcc gctacacggc cccgccacct ccgaacctgg cgctgggtgc ccgggcgagc
     2521 tcgtcctcgg actggtcggc cgagcatgcg gcggccaagg tcagcgacgg gcgagccgac
     2581 actcggtgga acagcgccca tggcgaccgc gacggcgcct ggatcgccct cgagtggccc
     2641 tcggctgtcg tgtgcgacgc cgtcaggatc cgcgaagcgc cggacctcgg tccgcgcgtt
     2701 cagacgtggg tgctcgaggg cttcgacggc acgcgatgga tccggttggc tgacggtggg
     2761 ccgatcggtg cggcccacac ggcgacgttc gcgcagcgct cgttgctgcg gctgcggctg
     2821 accatccggt cggcctccga cgtgcccacg ttcacggaga tagaggtcta tgaccggggc
     2881 ggctgacgcg tcactccaca agacg
//
LOCUS       DAOSPL010000480         2899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_34352_length_2899_cov_3.107595, whole genome shotgun sequence.
ACCESSION   DAOSPL010000480 DAOSPL010000000
VERSION     DAOSPL010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34352_length_2899_cov_3.107595"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PLQ54_09570"
     CDS             complement(<1..819)
                     /locus_tag="PLQ54_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOS93548.1"
                     /translation="MRQLHRLEALVPNCTAVAISPDGRLLAAGRTDGTIALVIPANGS
                     IQGSFGGHGGRVSSLAFSPDGQWLASASDDGAVRCSDTHSLELLGLLDGIREPASLAF
                     SADGTRLAVACGFGHEFGPAAQAAPHAKYAYGVQVLEAPALKPLWGSLAHTERATGVS
                     IASTGLYALSSSLDGHVVMYAMQTGKVSKRENLNAPLYGSVYRPDAKRAYLPAGLPLR
                     GAASAGGLDERPMPVFVVNGLTLEVLRPLAGHTGLPTGGVWSAAVGQGLIVTGGS"
     gene            complement(832..2256)
                     /gene="proS"
                     /locus_tag="PLQ54_09575"
     CDS             complement(832..2256)
                     /gene="proS"
                     /locus_tag="PLQ54_09575"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOS93549.1"
                     /translation="MQFIANRDEDYAQWYVDVVLKSKLADYAPVGGCMVIRPYGYALW
                     ENMVAALDGMFKETGHENMYFPLMIPESFLKKEAEHVEGFAPECAVVTHGGGKKLEEP
                     LVIRPTSETVIWSMYKKWIHSYRDLPILGNQWANVVRWEMRTRLFLRTTEFLWQEGHT
                     AHATEREADEEALRMLEVYRRFAEDYMALPVVCGEKTERERFAGAVRTYCIEAMMGDR
                     RALQAGTSHFLGQNFAKAFDVTFQNESGEHEYVWGSSWGVSTRLVGALVMGHGDEKGL
                     ALPPRLAPIQVVVVPIYRKDEEKARVLEVAHQIHARLAKNVRVHVDDRDQLKPGFKFN
                     EWELCGVPLRLELGPRDLENGQVTFARRDTGDKGTVPLDALESEVPTLLDMVQTGLFD
                     RALEFRENNTCTTDSWTEFREQLEAGGFVYAHWNGSGETEARIQEETKATIRCIPFGR
                     QPEAGKCILTGEPSEGRVLFARAY"
     gene            complement(2497..>2899)
                     /locus_tag="PLQ54_09580"
     CDS             complement(2497..>2899)
                     /locus_tag="PLQ54_09580"
                     /inference="COORDINATES: protein motif:HMM:NF040071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HOS93550.1"
                     /translation="GVSPRLMAGAPVTDDVLKCQRKPIDAADYLPYNDYAYVSVSNGA
                     NEVLWQKSVGDVGDLGDGVPEAWSWTAPAAGTYTLQYGVVNSGDRINPSYGLFDVGGL
                     NYQPTPEPATCGLLLVSGGTFLLRRRTRARR"
BASE COUNT          484 a          960 c          977 g          478 t
ORIGIN      
        1 ggagccgccg gtgacgatga gcccctgccc gacggcggcc gaccatacgc ctccggttgg
       61 caagcccgta tggccggcga gcgggcgcaa cacctcgagc gttaggccgt tgaccacgaa
      121 cacgggcatg gggcgttcgt cgagcccacc ggcgctggcg gcgccccgca acgggagtcc
      181 cgcgggcaga taggcgcgct tggcgtcggg ccggtagacg gaaccgtaaa ggggggcgtt
      241 cagattctcg cgcttgctca ccttgcccgt ctgcatggca tacatgacga cgtggccatc
      301 gaggctgctc gacagggcat acaggcccgt cgaggcgatg ctgaccccgg ttgcccgttc
      361 cgtgtgagcc agggagcccc agaggggctt gagggccgga gcctcgagca cctgaacgcc
      421 gtaggcatac ttggcatgag gcgcggcctg ggccgcgggt ccgaactcgt gcccaaagcc
      481 gcaggccacg gcaagacggg tgccgtccgc cgagaaggcc aggctggcgg gctctcggat
      541 cccgtcgaga aggcccagga gttccagcga gtgcgtgtcc gagcagcgca cggcaccatc
      601 atcggaggcc gatgcgagcc actggccgtc cggcgagaaa gcgagtgagg acacacgccc
      661 tccgtggccg ccgaaggagc cctggataga cccgttggcg gggatcacca gcgcgatggt
      721 gccgtcggtg cgacccgcag ccagtaggcg gccgtcgggt gagatcgcca ccgcggtgca
      781 gtttggaaca agcgcctcaa gtcggtggag ctgacgcatg gcaatacccc ctcagtaggc
      841 tcgcgcgaac agcacccgcc cctccgatgg ctcgcccgtc aagatgcact tgccggcctc
      901 gggctgtctc ccgaagggga tacagcggat ggtggccttg gtctcttcct ggatgcgggc
      961 ctcggtctcg ccggagccgt tccagtgggc atagacgaag ccgccggcct cgagctgctc
     1021 gcggaactcg gtccaactat cggtggtgca cgtgttgttc tcacggaact cgagcgcgcg
     1081 gtcgaacagc ccggtctgca ccatatcgag caacgtcggc acctccgatt cgagcgcatc
     1141 cagcggcacg gtgcccttgt cgcccgtatc gcggcgggcg aaggtgacct ggccgttctc
     1201 gaggtcccga gggcccagct cgagccggag cgggacgccg cacaactccc actcgttgaa
     1261 cttgaagccc ggcttgagct ggtcgcgatc gtccacgtgg acgcggacgt tcttggccag
     1321 gcgcgcgtgg atctgatggg cgacctcgag cacgcgcgcc ttctcctcat ccttgcggta
     1381 gatgggaacc acgaccacct ggatgggcgc gaggcggggg ggcagagcga ggcctttctc
     1441 gtcgccgtgc cccatgacca gagcaccgac cagccgtgtg ctgacacccc agcttgagcc
     1501 ccagacgtac tcgtgctcac cactctcgtt ctggaacgtg acatcgaagg ctttggcgaa
     1561 gttctgaccg aggaagtgcg aggtgcctgc ctgcagggcc cggcggtcac ccatcatggc
     1621 ctcgatgcag taggtgcgca cggctccggc gaagcgctcg cgctcagttt tctcgccgca
     1681 gaccaccggc agcgccatgt agtcctcggc gaatcgccgg tagacctcca gcatgcgcag
     1741 cgcttcctca tccgcctcgc gctcggtggc atgggccgtg tggccctctt gccaaaggaa
     1801 ctcggtggtg cgaaggaaca ggcgagttcg catctcccac cgcaccacgt tggcccactg
     1861 gttgcccaga atcggcagat cgcggtacga gtgtatccac ttcttgtaca tggaccagat
     1921 gaccgtctcg gaggtcggcc gaatcaccag gggctcttcg agcttcttgc cgccgccgtg
     1981 ggtcaccacg gcgcactcgg gggcgaaacc ctcgacgtgc tcggcctcct tcttgaggaa
     2041 gctctcgggg atcatcagag ggaagtacat gttctcgtgg ccggtctctt tgaacatgcc
     2101 atccagcgcc gcgaccatgt tctcccacag ggcgtagcca tacggccgga tgaccatgca
     2161 gccgcccacg ggggcataat cggccagctt ggacttgagc accacgtcga cataccactg
     2221 ggcgtagtct tcgtcgcggt ttgcgatgaa ctgcatcggc ctgctgccat cctgagtcga
     2281 catggtcatc ctcactggga gagtctgctc gcgcgccgcc gtgtcgaggt cgtggagctg
     2341 agtcggctcc tgccgcacac gcggaacggc gaggctacag cgtagcagac cgaggcaacc
     2401 gcgccaaccg tgcacgcaga gcccgggccg ggggacgcgg gctctgcggt tggggggaac
     2461 ccggatgagc gcgcggcggc tttcgccgaa gcccggctac cgacgggcgc gcgttcttct
     2521 cctcaacaga aacgtcccgc ccgagacgag cagcagtccg catgtggcag gctcaggggt
     2581 gggttggtag ttcagcccgc cgacatcaaa cagtccgtag ctggggttta tccggtctcc
     2641 ggagttcacc acaccgtact ggagcgtgta ggtgcccgca gcgggcgcgg tccatgacca
     2701 tgcctcaggg acaccatcgc ccaggtcgcc gacgtcgcca acggatttct gccagagcac
     2761 ctcgttggcg ccgttcgaca ccgacacgta ggcgtagtcg ttgtacggca ggtagtcggc
     2821 cgcgtcgatg ggcttgcgct ggcacttcag cacgtcgtcg gtcaccggcg cgccggccat
     2881 gaggcggggc gacacgccc
//
LOCUS       DAOSPL010000481         2898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_34368_length_2898_cov_2.781217, whole genome shotgun sequence.
ACCESSION   DAOSPL010000481 DAOSPL010000000
VERSION     DAOSPL010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34368_length_2898_cov_2.781217"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..1521
                     /locus_tag="PLQ54_09585"
     CDS             205..1521
                     /locus_tag="PLQ54_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOS93551.1"
                     /translation="MRAAWRAWLICISILGWAWLFTAQSNAQVLIPVGGTGPAPGRTS
                     TAEAVEPAPPLALTVRDKPNDDATALCLSWELSEDDGPAGRVTGYVVERSAGADGPFD
                     EVGRVGRGTATFTDSAYSWKPSERAKERGEGPRLADPAQPHWYRVRALSERSQSAPVT
                     AGPVRPVETGFDTTRGSVLLVLTLCAGMVLFLIRHAGTRDLYIRPIPGLEALHDAVGR
                     ATEMGKPILYSTGMGSSTLIDTIAPTMAATVLLGQIARLAAEQGTRILVPNIDPLIMS
                     AQREVVRDAYAQAGRPEAYREEDLFFQSNAQFAYAAMVTGIMLRDRPAACFYLGYFEA
                     ESLILAETGALTGAIQIAGTDQVFQLPFFVTACDYTLMGEELYAATAYVSRAPRLLGS
                     LKAQDWLKFAVGAVILLGVAGALLTHVFHLPPDSWFPRFSQWMGGA"
     gene            complement(1667..1870)
                     /locus_tag="PLQ54_09590"
     CDS             complement(1667..1870)
                     /locus_tag="PLQ54_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93552.1"
                     /translation="MSSGDRSGEVIGPAPRLSGKLTDLRGSLGTQVAAASGHKRLRLD
                     LAVRHIWPVSPQISSRGTSRVIL"
     gene            1992..>2898
                     /locus_tag="PLQ54_09595"
     CDS             1992..>2898
                     /locus_tag="PLQ54_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93553.1"
                     /translation="MPKRSVLGGGVALLLWASAAASGVEPPAPREAPSVSVPVAELPP
                     LAPAELPLAPPAAVEAKDKPNDDATAIDLSWRPSPDDAPDSQRVMGYVVRRAEAPDGP
                     YVVVGRVFTKGEGKFTDSSFSWKSKDREPPESAPPSPGGQGPAAEPSAGIPTDPAPVR
                     ADPRGQYYYEVAAFGLSEGRYEESAPSRAGPVTPREYWLDRDQINVLVGTVLCTIVVL
                     GFLGFARRGRDFYVRPIAGLKAVDDAIGRATEMGRPLLFVAGLGGVSDIATVAAMAIL
                     GRIGRTAAEQGTRILVPCRDPMVMTA"
BASE COUNT          421 a          974 c         1012 g          491 t
ORIGIN      
        1 caagcgcacc accttgatcg aaacgttggc acgggtcggt tggcccgcgt catcgctctg
       61 ccgcccgaac tgacgcgcat cgagaccgag gcacacgtgc gggtggttac cgtccagacg
      121 gaggacggcg ttgccgtgac cctccctcgg gccaacatcg agctgatccg ctgactcgag
      181 tcgcatcgga gcagggagat gcccatgcgg gcggcttggc gggcgtggct gatctgcatc
      241 tccatcctgg gctgggcctg gcttttcacc gcgcagtcga acgcgcaggt actgatcccc
      301 gtgggcggga ccggtcctgc cccgggccgg acctcgaccg ccgaggcggt cgagccggct
      361 ccaccccttg cgctgaccgt gcgggacaag cccaacgatg acgccacagc cctgtgcctg
      421 tcgtgggagc tttcggagga cgacgggccc gcgggccgcg tcaccggcta cgtggtcgag
      481 cggtccgccg gcgccgacgg accgttcgat gaagtgggcc gtgtcggtcg cggtacggcc
      541 acgttcacgg acagcgccta tagctggaag ccgtccgagc gggccaagga gcgtggggag
      601 gggccccggc tggccgaccc cgcccagccg cattggtacc gcgtcagggc cctgagcgag
      661 cgcagccagt cggcccccgt gaccgccgga cccgtgcggc ccgtcgagac cgggttcgac
      721 acgacccggg gcagcgtcct actggtgttg accctatgcg cggggatggt cctcttcctg
      781 atccgccatg ccggcacgcg tgacctgtac atccggccca tcccggggct cgaagccctg
      841 cacgatgcgg tgggccgggc caccgagatg ggcaagccga tcctctactc caccgggatg
      901 ggctcgtcaa cgctcatcga caccatcgcg ccgacgatgg ccgcaacggt gctgttggga
      961 cagattgccc gtctggctgc cgagcagggc acgcgcattc tggtgcccaa catcgatccg
     1021 ctcatcatga gcgcccagcg ggaggtcgtc cgcgacgcgt acgcccaggc tggacggcca
     1081 gaggcctacc gcgaagagga cctgttcttc cagagcaacg cgcaattcgc ctatgccgcc
     1141 atggtcaccg gcatcatgct gcgggatcgg cccgcggcgt gcttctacct cggctacttc
     1201 gaggccgagt ccctgatcct ggccgagacc ggcgcgctca cgggcgccat ccagatcgcc
     1261 ggcaccgatc aggtgttcca gctaccgttc ttcgtcacgg cgtgtgacta cacgctcatg
     1321 ggcgaggagc tgtacgccgc caccgcttac gtgtcgcgcg cgccgaggct tctcggaagt
     1381 ctcaaggcgc aggactggct caagtttgct gtcggtgccg tcattctctt gggtgttgcc
     1441 ggcgccctac tgacccatgt gttccacctg ccgcccgact cgtggttccc tcgcttctct
     1501 cagtggatgg gcggggcgta gggcagatgg cagatggcta gacgaagcgc ggtaggctcg
     1561 gcgttggagg cgctggcgag tcagggtggg gggggcccga gttgccggca agagaccagc
     1621 gggatctggt tgcttggcgc aaagcgatgg acttggttga tgtcgtctac aggatgacgc
     1681 gggacgtccc gcgcgacgag atctgtgggc tcacgggcca gatgtgccgc accgcgaggt
     1741 cgagacgcag gcgtttgtgg cccgacgccg cggctacctg agtgccgagt gagccacgca
     1801 ggtcggtgag cttgccggag aggcggggcg ccgggccaat gacctcgccc gatctgtccc
     1861 ccgaactcac cgctagcggg attggctccg cctgcggctg ccatctgcca tctgcctctg
     1921 aaggtctgcc ccgggcctcg ctgaatacac tccgggcagc aagaccggcc aatgtgacgg
     1981 ggaggtgcgg tgtgccgaaa cggtccgtgt tagggggcgg agtcgctctg ctgctgtggg
     2041 ccagtgcggc ggcgtcagga gtcgagccgc ccgcgccccg cgaagctccc agcgtgagcg
     2101 ttcccgtcgc cgagctccct cccctcgctc cggcggagct gcccctcgct ccgccggcgg
     2161 cagttgaggc caaggacaag cccaacgacg acgccaccgc catcgacctc agctggcggc
     2221 cttcgcctga tgacgccccc gacagccagc gcgttatggg ctatgtggtc cgtcgcgctg
     2281 aggcccccga tggcccctat gttgtggtgg ggcgcgtatt caccaagggt gaagggaagt
     2341 tcacggattc gtcattcagt tggaagtcca aggatcggga accgcccgag tcagcgcccc
     2401 cctcgccggg cggccaaggg cctgccgcgg agccgtcggc cgggattccc acggacccgg
     2461 cgccggtgcg cgcggacccg cggggccagt actactatga ggtcgccgct ttcgggctga
     2521 gcgaggggcg ctacgaggag tcggccccgt cacgggcagg gcctgtcacg ccgcgcgagt
     2581 actggctcga ccgcgatcag atcaatgtgc ttgttggcac cgtgttgtgc acgatcgtgg
     2641 tgctggggtt cctcggcttt gcgcgccgcg gccgtgactt ctacgtgcgg cctatcgctg
     2701 gcctcaaggc ggttgacgat gccatcggcc gcgcgaccga gatgggtcgt cctctgctgt
     2761 tcgtggccgg tctcggtggg gtgagcgata tcgccaccgt ggccgccatg gccattcttg
     2821 gccgcatcgg tcgcacggca gctgagcagg gcacacggat tctcgtgccg tgtcgcgatc
     2881 ccatggtcat gacggccc
//
LOCUS       DAOSPL010000482         2894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_34433_length_2894_cov_2.957027, whole genome shotgun sequence.
ACCESSION   DAOSPL010000482 DAOSPL010000000
VERSION     DAOSPL010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34433_length_2894_cov_2.957027"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PLQ54_09600"
     CDS             <1..203
                     /locus_tag="PLQ54_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93554.1"
                     /translation="ALAEAEAAERRGASLPMGTLRARASLAEAVALERASRYALSVAY
                     AAWARSTGATAEVFLTAGPPEG"
     gene            229..2079
                     /locus_tag="PLQ54_09605"
     CDS             229..2079
                     /locus_tag="PLQ54_09605"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOS93555.1"
                     /translation="MPKRRGVSIAVSAAVTVVVVGGLLATRRPWGARAGAAVTSGSGT
                     IEATEVEVSAKIPGRLAQVLAREGDTVHKGDVIAVLDHGDLDAEVERAEGALASAEAF
                     VKELRRGSRSQQIAAARARVAQTQAALNGARSQLSIAQASYDKSTELKQTVDQAQAQV
                     ASAEAALAAARARRDEALAGPGRQERQRADAERRRAQAGLEGAEAGARDAEELLRLQR
                     DFTGPTTEAETAERTARLAVGIAEEQRRLVDEGARSQQVAQAESTLEAATIAYENARQ
                     RYARVKGLFEDKAATQQDYDDAKASLDGARARRDAAQAALADLRSGARPQERRSAALS
                     VERASAAHAGAERGVDNARIAQEQAVLTAQMKAAAAQAERRAAQAALEAADATVDMVN
                     EGTRPEQVAQAEAAVRQAEALLDGARTALANAKEARADRFLAQQQLETARTQLALAEA
                     QQTAAQAELSLVMEGSTAEAIEAAEGQVRQAKGALDAARIRRQEAEVRAPCAGTVSDV
                     VAEAGEVVAAGAAIVSMLDLEHMWLKVYLPVSQLHRVAVGDRATVRIDAEPRERIPGT
                     VTAVARESEFTPKNVQTIEQRTRLVYWVKVGVGDGPGLLQPGMPADATFE"
     gene            2076..>2894
                     /locus_tag="PLQ54_09610"
     CDS             2076..>2894
                     /locus_tag="PLQ54_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS93556.1"
                     /translation="MSPVAPGASGDGLLIAPAAPIVAVEHLAKRFGTIEAVRDVSFAV
                     APGEVFGLAGPDGAGKTTTMRILAGIMAPDGGSAVVAGIDVLRRPEAAKGQIGYLSQA
                     FSMYSDLTVAENVAFFGDLYEVPRNTLAERREMLLRMTGLEPFQRRLAEHLSGGMRQK
                     LALCCTLIHRPRVLLLDEPTTGVDPASRRDFWRLLLRFTREGVTLVVSTPYMDEVSRC
                     DRVGLMYAGSVLALDTPDSLPATAGPVVEITSDHPLELQASLRQRSDVWAAEVFG"
BASE COUNT          449 a          926 c         1123 g          396 t
ORIGIN      
        1 aggctctggc tgaggccgag gccgccgagc gccgaggcgc ctcactgccg atggggacct
       61 tgagggcccg cgcgtcactg gccgaggcgg tggcgctcga gcgcgccagc cgctatgcgc
      121 tgtcggtggc ctatgccgcg tgggcgcggt ccaccggcgc caccgccgaa gtgtttctga
      181 ccgcaggacc tcccgagggg tgaggaggat gttgaggagg attcccgagt gccaaagcgg
      241 cgcggggtgt cgatagccgt aagcgcggca gtgactgtcg tggtcgtcgg gggcctgttg
      301 gccacccgca gaccttgggg ggcccgcgcc ggtgcggcgg tcacctccgg gagcggcacg
      361 atcgaggcga ctgaggttga ggtctcggcg aagatcccgg ggcgattggc ccaggttctg
      421 gcacgcgagg gtgacacggt ccacaagggc gatgtcatcg ccgttctgga tcacggcgac
      481 ctcgatgccg aggttgagcg cgcggagggc gccctggctt cggccgaggc gtttgtgaag
      541 gagcttcggc ggggatccag gtcgcagcag attgccgcgg cgcgggcgag ggtggctcag
      601 acccaagccg cactgaacgg cgcgcgcagc cagctgagca tcgcgcaggc aagctacgac
      661 aagagtacgg agctcaaaca gacggtcgac caggcccagg cgcaggttgc ctcggccgag
      721 gcggccctcg cggcggcccg ggcccggcgg gacgaggccc tggccgggcc aggccggcag
      781 gagcgtcagc gggccgatgc cgagcggcgc cgcgcccagg cagggctgga gggtgccgag
      841 gctggcgcgc gggacgccga ggagctgctg cggcttcagc gtgacttcac cggccccacc
      901 accgaggcgg agaccgcgga gcgcaccgcc cgcctggcgg tcgggattgc ggaggagcag
      961 cggaggctgg tggacgaagg ggcgcgcagt cagcaggtgg ctcaggcaga gtctacccta
     1021 gaggcggcga ccatcgccta cgagaatgcc cggcagcgat atgcgcgggt caaggggctg
     1081 ttcgaggaca aggcggcgac gcaacaggac tacgacgacg ccaaggcgag cctcgacggc
     1141 gcccgcgccc gcagggatgc cgcgcaggcg gcgctggccg accttcgcag tggcgcccgg
     1201 ccgcaggagc ggcgctccgc ggccctgtca gtcgagcggg cgtcggcggc gcacgcgggg
     1261 gccgagcggg gtgtggacaa cgcgcgcatc gcccaggagc aggcggtcct gaccgcccag
     1321 atgaaggcgg cagctgccca ggcagagcgc cgggcggcgc aagcggctct ggaggctgcc
     1381 gatgcgacgg tggacatggt aaacgaggga acgaggcccg agcaggtggc ccaggccgag
     1441 gcggcagtgc ggcaggctga agcccttctg gatggagcgc gcacggccct cgccaacgcg
     1501 aaggaagcgc gggccgatcg cttcttggcc caacagcagc tcgagacggc gcgcacccag
     1561 ctcgctctgg ccgaggccca gcagacggcg gcccaggccg agttaagcct ggtgatggag
     1621 ggcagcacgg ccgaagccat cgaggcggcg gaaggccagg ttcgccaagc caagggcgcg
     1681 ctggacgcgg ctcggattcg gcggcaagag gcggaggtgc gcgcaccgtg cgcgggcacc
     1741 gtatccgacg tcgtcgccga ggccggcgaa gtggttgcgg ccggcgctgc catcgtctcc
     1801 atgctggatc tcgagcacat gtggctgaag gtctacttgc cggtgtccca gctccatcgt
     1861 gtggccgtgg gcgaccgggc cacggtgcga attgacgcgg agccccgtga acgcataccc
     1921 ggaacggtca ccgccgtggc tcgcgagtcg gagttcacgc ccaagaacgt gcagaccatc
     1981 gaacagcgga cccgcctcgt ctactgggtg aaggtgggcg tcggagacgg gccggggctt
     2041 ctccagcccg ggatgcctgc cgacgcgacg ttcgaatgag ccccgtggcg cccggtgcca
     2101 gcggcgatgg cctgctgatc gctcccgcgg cccccatcgt ggcggtggag cacctggcga
     2161 agcggttcgg gaccattgag gctgtccgcg acgtcagctt cgccgtggcg cctggtgagg
     2221 tcttcgggct cgcgggcccg gacggcgcgg gcaagacgac caccatgcgc atcctcgcgg
     2281 ggatcatggc gcccgacggc ggctcggccg tcgtagccgg gattgatgtc ctgaggcggc
     2341 cggaggccgc caaagggcag atcggctacc tctcccaggc attcagcatg tacagcgacc
     2401 tcaccgtggc cgagaacgtc gccttcttcg gtgatctgta cgaggttccg cgcaacacac
     2461 tggccgagcg gcgggagatg ctgctgcgga tgacgggtct cgagccattc cagcggcggc
     2521 tggccgagca tctctcaggg ggaatgaggc agaagctggc cctgtgctgc acgctgattc
     2581 accgaccccg ggtgctgctg ctggatgagc cgaccaccgg agtggatccg gcttcgcgtc
     2641 gcgacttctg gcgcctcctg ctccgtttca cgcgtgaagg tgtcacgctg gtcgtcagca
     2701 cgccgtacat ggacgaggtt tcccggtgtg accgggtagg gctgatgtac gcggggtcgg
     2761 tgttggctct ggataccccc gattcactgc ccgcgacggc cggccctgtc gttgagatca
     2821 cttcagacca tccgctggag ctgcaggcgt cgctgcgcca acggagcgac gtgtgggcgg
     2881 cagaggtgtt cggg
//
LOCUS       DAOSPL010000483         2892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_34465_length_2892_cov_3.235812, whole genome shotgun sequence.
ACCESSION   DAOSPL010000483 DAOSPL010000000
VERSION     DAOSPL010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34465_length_2892_cov_3.235812"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..457
                     /locus_tag="PLQ54_09615"
     CDS             122..457
                     /locus_tag="PLQ54_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93557.1"
                     /translation="MSPRGALAVLTVLLLGVSLSACGCARRGSGEGALDVRQPSTVAG
                     KAAQPGVAPGPSAAVRRDTPLPPGGGLTKPETQPGPLLGRDEYVLPGPDAKTETDSAS
                     GEPAARPGG"
     gene            581..1579
                     /locus_tag="PLQ54_09620"
     CDS             581..1579
                     /locus_tag="PLQ54_09620"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS93558.1"
                     /translation="MTGRDRVSLALNHQEADRVPRYIWVDGGLATERIVARFGTFDAF
                     LDHLDVDMFQAFPAGPGIIDWEAVTARPGEARSQGLSQGALTLDQALEAPLTDPNTPS
                     IYEPIRAAIAHHQQGRGRAVFVQTPGCFETANGIIGMDTQFMEMALRPEGMRQLYRRQ
                     LEWTRVYIDNCLDLGADVVHISDDMGQNGRMLFSPQTFREVVAPVLAEEAAHVRRRSP
                     QLSLHSCGYFDDVIGDLADMGFTCLHPIQESAGMDIPSVKARFGGRVTIYGGLDVRST
                     LPSGDRDRIRADLERVVGAAKPGGGLIFCTGHTVHSDCDLADVLFAYELLDELGRY"
     gene            complement(1583..>2892)
                     /locus_tag="PLQ54_09625"
     CDS             complement(1583..>2892)
                     /locus_tag="PLQ54_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93559.1"
                     /translation="IQVVDQPGLLDMVAFADQEYEGVGYQWLGKTEIRAELHTGGKRE
                     LLLRLLWGAKGDERRARVKAGPVDMVLTRGGYAGFQWLEVPVVLTDAMPDLSVELLRE
                     GDGPHAFVSQIQLVDPAVAGQAARDAARQRRVSDTWRAAPKVLMIVHDPVIPAKGNRP
                     LHKAFGWNDPDELAREYIADVREASGGMVRYEVAKRLVVDGFPTKADGFRYDADGYVR
                     AWDERAFHQPDGVDYRRLIEEFGVIDLVEKGAIDEVWLFVAPYFGYYESLMVGDGAYW
                     CNSPPLEGIRCKRRFVVMGFNYERGVGEMLEDLGHRTESILTHVYGSWDPEETHAWNR
                     FTLHDKALPGRAACGNVHFAPNSPSDYEWGDRRFVQSTCDAWYGYPDVSGEPRWVNCE
                     EWGNGDIRAHHKWWFDHLPRAPGKADGKWNNWWRYVIDLNGPE"
BASE COUNT          440 a         1043 c          946 g          463 t
ORIGIN      
        1 ggtgggacgt ccgcttcagc gcgaagggcg acgggggtcc ccggggcctg ctcgccagcg
       61 tgaccgtcga tgcccacacc ggcaaggtcg ctcacgtggt tcccttcgag gggagacgcc
      121 catgagtccc cgaggggccc tggcggtcct gactgtgttg ctcctgggcg tgtccctgtc
      181 ggcatgcggc tgcgcgaggc ggggatcggg tgaaggcgcc ctcgatgtcc gccagccctc
      241 cacggtcgct ggcaaggcgg cccagcccgg cgtcgcccca ggcccgagcg ccgccgttcg
      301 ccgagacacg cccctgccgc ccggaggagg gctcaccaag ccggagaccc agcccggccc
      361 cctgctcggg agggacgagt acgtgctacc cgggcccgat gccaagaccg agaccgacag
      421 cgcgtcgggc gagccggcgg ctcggcccgg cggctagcgg ccggcctagg ccacggcatg
      481 tgcccgcacc gacgcgatcc ccgtcacgcg agtggaacag aaccgcgcgg cacggtatcc
      541 tgcgcagggg cgtggctcag ccccagccgg gagggctggt atgactggac gcgatcgtgt
      601 atcgctggca ttgaaccacc aggaggccga ccgggtgcct cgctacatct gggtcgatgg
      661 cggcctggca accgaacgca tcgtggctcg cttcgggacc ttcgatgcgt tcctggatca
      721 cctggatgtg gacatgttcc aggccttccc cgcggggccg gggatcatcg attgggaggc
      781 ggtgaccgca cggcccggcg aagcccgctc gcaggggctc agccaaggcg ccctgacgct
      841 cgatcaggcc ctcgaggcgc cgctcaccga ccccaacacg ccttcgatct acgagcccat
      901 ccgcgcggcc atcgcccacc atcagcaggg acgcgggcgc gccgtgttcg tccagacacc
      961 ggggtgcttc gagaccgcca acggcatcat cggcatggac actcagttca tggagatggc
     1021 gctgcggccc gaggggatgc ggcagctcta ccggcgccag ctcgagtgga cgcgcgtcta
     1081 catcgacaac tgcctggact tgggggccga tgtggtccac atctccgacg acatggggca
     1141 gaacggccga atgctgttct cgccgcagac attccgcgag gtggtcgcgc cggtcctcgc
     1201 cgaggaagcc gcccatgtgc gccggcggag cccccagctc tcactccact cctgcggcta
     1261 cttcgatgac gtcatcggcg acctggccga catgggcttc acatgcctgc accccatcca
     1321 ggagtcggcg gggatggaca ttccgagcgt caaggcgcgg ttcggcggcc gggtgaccat
     1381 ctacggcggg ctcgatgtgc ggtccacgct ccccagcggc gaccgggatc ggatccgcgc
     1441 cgacctggag cgcgtcgtcg gtgccgccaa gcccggcggc gggctcatct tctgcaccgg
     1501 gcacacggtc cacagcgact gcgatctcgc cgatgtgctc ttcgcctatg agttgctgga
     1561 cgagctggga cggtactgac ctctactccg gcccgttcag gtcgatgaca taccgccacc
     1621 agttgttcca cttgccgtct gccttgcccg gcgcccgcgg caggtggtcg aaccaccact
     1681 tgtggtgagc gcggatgtcg ccgttgcccc attcctcgca gttcacccat cgcggctcac
     1741 cggacacgtc cgggtagccg taccacgcat cgcacgtgct ctgcacgaac cgccgatctc
     1801 cccactcgta gtcgctcggg ctgtttggcg cgaagtggac attcccgcac gccgccctgc
     1861 ccggcagcgc cttgtcgtgg agtgtgaagc ggttccacgc gtgggtctcc tcgggatccc
     1921 aactgccgta cacgtgcgtc aggatggact cggtgcggtg ccccaggtcc tcgagcatct
     1981 cgccgacgcc gcgctcgtag ttgaagccca tcaccacgaa cctgcgcttg caccgtatgc
     2041 cctcgagtgg gggcgagttg caccagtagg caccgtcgcc caccatcagc gactcgtaat
     2101 aaccgaagta gggagccacg aacagccaca cctcgtcgat ggcgcccttc tccaccagat
     2161 cgatcacgcc gaactcctcg atcagacgcc ggtagtccac gccgtcgggc tgatggaacg
     2221 cgcgctcatc ccaggccctg acatacccgt cggcgtcgta ccggaacccg tcggccttgg
     2281 tcgggaagcc gtcgaccacc aggcgcttcg ccacctcata gcgcaccatt ccgcccgagg
     2341 cctcacgcac atcggcgatg tattcccggg cgagctcgtc ggggtcgttc cacccgaagg
     2401 ccttgtggag cggtcggttg cccttggctg ggatcaccgg gtcgtggacg atcatgagca
     2461 ccttcggtgc cgcccgccag gtgtcgctca cccggcgctg gcgcgctgcg tcgcgggccg
     2521 cttggccggc gacggccggg tccacgagct ggatctgtga gacgaatgcg tggggtccgt
     2581 caccctcgcg caggagctcg acgctgagat caggcatcgc gtctgtgagc accacgggta
     2641 cttccagcca ctggaagccc gcatagcccc cgcgagtcaa gaccatgtcg accggtccgg
     2701 ccttcacccg ggcccggcgc tcatcgccct tggcgcccca gagcagccgc aggagcagct
     2761 cgcgcttccc tccggtgtgc agctcggctc ggatctcggt cttgcccaac cactggtagc
     2821 ccacgccctc atactcctgg tcggcgaagg ccaccatgtc gagcagcccg ggctggtcga
     2881 caacctggat cg
//
LOCUS       DAOSPL010000484         2884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_34581_length_2884_cov_3.521032, whole genome shotgun sequence.
ACCESSION   DAOSPL010000484 DAOSPL010000000
VERSION     DAOSPL010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34581_length_2884_cov_3.521032"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..2383
                     /locus_tag="PLQ54_09630"
     CDS             179..2383
                     /locus_tag="PLQ54_09630"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOS93560.1"
                     /translation="MLPLLSALPSALALMATPTPTPNWIWIEGEGPPTWAPAADRYFR
                     RTFELDDVPAKVELAITADDTFDATINGKPAAQGTSWEQLYHVDIAPLLRRGTNVLAV
                     CAKSTLPGPGALVVRITGLAEPLVSDARFRVTRDAPAEGWREVGLDDSSWEHALVIGP
                     LGCPPWGSGIADQFEGAHLIEDIIAAYRSDDPVLVVPTPRGRLSDKRDVTLGRLGRPL
                     GVRYDGVPADSYAIELLDRGLAAFGVAVEPRAASPAIRLVLAPQRDSRPQAYSLDIRQ
                     DEGVRIRAADTTGLVYGVATLLQMLRVNDGQLVAPDANLDDYPAAEIRGPVVAPYSPN
                     WLDFGAYFRLNAWFVGDVPPATVVSEAKRRGIGLMPARHLPDEYDFQSDEALNEIAQW
                     ALSAADAGLRWVTVNADDHPEKVYTEADRQRFGDELPGLAKAHAHFINRLKPLLGGRI
                     ELVFCPRVYYKIQAGTAYPRAHDERAYLENLAKGIEEPLLCWITEVSPSFLRESAERF
                     RTPPLAWHNFFPGDTTDWKVYYEAYPVVREPGSARGFFVLGNTQEPDLWRPNYLTFAG
                     NTWNPERPIGLREAFTALYGVRAGTALTRYAELAGGHDRPIGVMADFWDQPLEVAPRF
                     LGSGWAGVVPGAERTPELLARCQALATSAAEAAAMPLEAEGVPPAMAQRLTDEAMRTH
                     LAFSLWSHRIATELGQPWTGEDPAALRMRLADLIERLGLPGGATDLELTKGP"
     gene            2388..>2884
                     /locus_tag="PLQ54_09635"
     CDS             2388..>2884
                     /locus_tag="PLQ54_09635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS93561.1"
                     /translation="MADTWVCLVTYNEAANLPLVLEAVRVALPEAGIIVVDDNSPDGT
                     ASLARERAARDPGIHVIVREGKLGYASAQRAAIDFAVAQGAARVVTMDADLSHDPAAL
                     TALVAALNDADMAIGSRYVPGGATVGWPVHRQLLSRLGNLAFRWIGGLPARDNTSGYR
                     AYRTEA"
BASE COUNT          443 a         1084 c          933 g          424 t
ORIGIN      
        1 gggcttaagt gcggaccatt cgtgacagtc accggttttc agcctggcgg tgacctagcc
       61 cagaggcgca gtccggacgc ctcggagacg gcactgcccg gcctgccgcc ttccacaacc
      121 aggtctggcg accgcgggag gccctaggcc accgccagcg ccccggaaag gaccccccat
      181 gctccctctg ctcagcgcac taccgagtgc cctcgccctg atggccacac cgacgccaac
      241 acccaactgg atctggatcg agggagaggg acctccaaca tgggccccgg cggccgaccg
      301 ctactttcgc cggaccttcg agctcgacga cgttcccgcg aaggtcgaac tcgccatcac
      361 ggccgatgac accttcgacg cgaccatcaa cggcaagccg gccgcccagg gcacgtcgtg
      421 ggagcagctg taccacgtcg acatcgcccc actgctccgc cggggcacca acgttctggc
      481 cgtgtgcgcg aagagcacct tgcccggccc gggagcgctg gtcgtgcgca tcacggggct
      541 ggcggagccc ctggtctccg acgctcggtt ccgcgtgacc cgcgacgctc ccgccgaggg
      601 gtggcgcgag gtcggcctcg acgactcctc gtgggagcac gcactcgtca ttggcccgct
      661 cggctgccct ccgtggggct ccggcatagc tgatcagttc gaaggcgcgc acctcatcga
      721 ggacatcatc gccgcctacc gctcggatga ccccgtgctc gtggtgccca ctccgcgcgg
      781 ccggctgagc gacaagcgag acgtcaccct gggccgcctc ggacggcctc tgggcgttcg
      841 gtacgacggc gttccggccg acagctacgc catcgagctg ctcgaccgcg ggctggcagc
      901 gttcggcgtg gccgtcgagc cccgcgcagc gagccccgcc atccggctgg tgctagcgcc
      961 gcagcgcgac tcccggcccc aagcctactc gctcgatatc aggcaggacg agggcgtccg
     1021 catccgcgcg gccgacacga cggggctggt ctatggcgtc gccacgctac tacagatgct
     1081 ccgcgtgaac gatggtcagc tcgtggcacc cgacgcgaat ctcgacgact acccagccgc
     1141 ggagatccgg ggcccggttg tcgcgccgta ctcgcccaac tggctggact tcggtgccta
     1201 cttcaggttg aacgcctggt tcgtcggcga tgtcccgcct gccaccgtgg tctccgaggc
     1261 caagcgccgt gggatcggcc tcatgcccgc ccggcatctg cccgacgagt acgacttcca
     1321 gagcgatgag gcgctcaacg agatcgccca gtgggcgctc tcggccgccg acgccggcct
     1381 ccgatgggta acggtcaacg ccgacgacca cccggagaag gtctacaccg aagcagaccg
     1441 ccagcggttc ggcgacgagt tgcccggcct cgccaaggcg cacgctcact tcatcaatcg
     1501 gctcaaaccg ctcctcgggg ggcgcatcga gctcgtcttc tgcccccggg tctactacaa
     1561 gatccaggct ggcacggcct acccgcgtgc ccacgacgaa cgggcctacc tcgagaacct
     1621 cgcgaaaggc atcgaggagc ccctcttgtg ctggatcacc gaggtctcac cctccttcct
     1681 gagagagtcc gccgagcgct tccggacgcc gccgctggcc tggcacaact tcttccccgg
     1741 cgacaccacc gactggaagg tctactacga ggcctatccc gtcgtgcgcg agcctggcag
     1801 cgcacgtggg ttcttcgtcc tcgggaacac gcaagagccc gatctgtggc ggcccaacta
     1861 cctgaccttc gccggtaaca cgtggaatcc ggagaggccc atcggcctgc gcgaggcatt
     1921 cacggcactc tacggcgtcc gcgcgggcac ggcgctgacg cgctatgctg agctcgccgg
     1981 cggacatgac cgccccatcg gcgtcatggc cgacttctgg gatcagccgc tcgaggtggc
     2041 tccgcgtttc ctgggctcgg gctgggccgg cgtggtgccg ggcgccgagc gcacgcctga
     2101 gctgctggcg cgctgtcagg ccctggccac ttcggccgcc gaggccgccg ccatgccgct
     2161 ggaggccgag ggcgtgcctc cggccatggc ccagcgcctc accgacgagg ccatgcggac
     2221 acacctggcg ttcagcctgt ggagccaccg tatcgccacg gagctcggcc aaccatggac
     2281 cggcgaggat cccgcggcac tccgcatgcg cctggccgac ctgatcgagc gcctcggcct
     2341 gcccggcggc gccaccgatc tcgagctcac caaagggcca tagaccgatg gccgatacct
     2401 gggtctgtct ggtgacctac aacgaggccg cgaacctgcc actggtactc gaagcggtac
     2461 gggtggctct acctgaagcg ggcatcatcg tcgtcgatga caactcgccc gacggcaccg
     2521 cctcgctggc ccgggagcgg gcggcccgcg acccgggcat ccatgtcatc gtccgcgagg
     2581 gcaagctcgg gtacgccagc gcccaacggg cggccatcga cttcgctgtg gcgcagggag
     2641 ccgcgcgcgt cgtcaccatg gacgccgacc tgtcgcacga cccggcggcc ctgacagcgc
     2701 tggtcgcggc gctcaacgac gcggacatgg ccatcggctc gcggtacgtg cccggcggcg
     2761 ctactgtcgg ctggccggtg caccggcagc tcctgagccg cctggggaac ctcgccttcc
     2821 ggtggatcgg cggcctcccc gcccgcgaca acaccagcgg ctaccgtgcc taccgcaccg
     2881 aggc
//
LOCUS       DAOSPL010000485         2879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_34670_length_2879_cov_3.365085, whole genome shotgun sequence.
ACCESSION   DAOSPL010000485 DAOSPL010000000
VERSION     DAOSPL010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34670_length_2879_cov_3.365085"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="PLQ54_09640"
     CDS             <1..579
                     /locus_tag="PLQ54_09640"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOS93562.1"
                     /translation="VAGPSPQRLMHVQKAFSAGVFRVYTSADIVGVELGGALKNVIAL
                     AAGISDGLGYADNTKASLITRGLAEITRLGVALGARPETFMGLSGLGDLVATCSSRTS
                     RNWRVGYALAQGVPLARAVDELGMVAEGVPTAQAARVLCQRTGTEMPIAAQVHSVLFE
                     AQDPRKAVEGLMARPLREEVLPDPPSTASTSS"
     gene            complement(566..2374)
                     /locus_tag="PLQ54_09645"
     CDS             complement(566..2374)
                     /locus_tag="PLQ54_09645"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOS93563.1"
                     /translation="MATASRKASVAELVDRLKSKNEAVQEETVQELIAAGERAVPELL
                     KAMSARAYQVRSCSARALGEIGDPRAVEPLVQALGDTSVNVQRSASEALAKLGSCATE
                     ALMLCLDSTDSLARKWAAEALGKIGDDSVVDSLIERLKERNVEVQKSIVVALGELKVK
                     RAIGPLIGFLEHENVDLARSAAESLGAIGSAKAVGPLLGALESPSLDVRRAAADALVS
                     IGAPSVEPLIEALGASPDYVVRRWAAEALGRLRDPRASPALMKALKDENMRVQRYAVV
                     ALGEVGEAKAVPKLLPFLKHESKDMRSAAVHALRLIGSSKAKEPLVASLTDSDWVVRL
                     SAAKALGELGDPSAAVHLEPLLSDPEWSVVLHTVRALGVLGHPQIVQKTAHLTRHERG
                     PIRAAVAEALGRANDPAALEVVHGMRNDDDARVREAVAEAMARLEQVVAQRVARGEPA
                     MPEAPQRPAKAAKPAAKAAAKPAAAKKAEPKAAKPAAKPAAKPAVKAAKPAAAAATKA
                     AKAAAKPAAKPAAKAAKPAAKAAAKPATKAAAKPAAKPAAKPAAAKTAAKAPAKAAAK
                     PAAKPAAKPTAKPAAAKTAAKAPAKAAAPKAPAKKK"
     gene            complement(2438..>2879)
                     /locus_tag="PLQ54_09650"
     CDS             complement(2438..>2879)
                     /locus_tag="PLQ54_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93564.1"
                     /translation="RAAVARLYRHGYARVFLAACLLLVAALTTVAMYQSRSANARSRD
                     DRLLRARTRASELTEAADALEGAAQRWSGPGDDGALGLVESAAKALSRAQRLSAGPPR
                     LRLQALQRDVDDLATLMRGGDPGAKAYLRQLAHSVRAAARSDGQ"
BASE COUNT          428 a         1103 c          882 g          466 t
ORIGIN      
        1 gtggccggac catcgccgca gagactcatg cacgtgcaaa aggcgttcag cgcaggggtg
       61 ttccgcgtct acacgagcgc cgacatcgtg ggcgtcgagc tcggcggggc actcaagaac
      121 gtcatcgccc tcgctgccgg aatcagcgac ggcctcggat acgccgacaa caccaaggca
      181 tcgctgatca cccggggcct ggccgagatc acccggctgg gcgtggccct cggtgcccgg
      241 cccgagacat tcatggggct ctccgggttg ggcgatctcg tggccacatg tagcagccga
      301 acgagccgca actggcgcgt gggctacgcc ctcgcccagg gcgtcccgct ggcgcgggcc
      361 gtggacgaac tgggaatggt ggcggagggc gtgcccaccg ctcaggcggc ccgtgtcctg
      421 tgccagcgca cgggcaccga aatgcccatt gccgcccagg tacactccgt gctcttcgag
      481 gcccaggacc ctcgcaaagc cgtcgaagga ctcatggccc ggccgctccg cgaggaagtc
      541 cttccggacc cgccctccac cgcatctact tcttcttagc cggtgccttc ggcgccgcgg
      601 ccttggccgg agccttcgca gccgtcttcg cagctgcggg cttggcagtg ggcttcgccg
      661 caggcttggc ggcaggcttg gccgccgcct tggccggagc cttcgcagcc gtcttcgcag
      721 ctgcgggctt ggcagcgggc ttggcagcgg gcttcgccgc agccttggtc gcgggcttcg
      781 ccgcggcctt ggccgcaggc ttggccgcct tcgcggcggg tttggcagcg ggcttcgccg
      841 cggccttggc cgccttggtc gctgcggccg ctgccggctt ggccgccttc acggcgggtt
      901 tcgcggcggg cttcgccgca ggcttcgcgg ccttcggctc ggcctttttc gccgctgcgg
      961 gcttggctgc tgccttggcc gcgggcttcg cggccttggc gggacgctgg ggcgcttctg
     1021 gcattgcggg ttcccctctc gctactcgct gggctacgac ctgctcgagc cgagccatcg
     1081 cctccgcaac ggcctcgcgt actcgagcat cgtcgtcgtt cctcatccca tgcacaactt
     1141 ccagtgcggc aggatcgttc gcacgtccca aagcttcagc aaccgccgcc cgaatggggc
     1201 cacgttcgtg acgagtcaaa tgtgcggtct tctgaacgat ctgagggtgg cccaacacac
     1261 ccagagcccg taccgtgtgc agcacgacac tccactccgg gtccgagagc aggggctcga
     1321 gatgaaccgc cgcgctcgga tcgcccagct cgcccagcgc cttcgcggca ctcagtcgaa
     1381 ccacccagtc actgtccgtc agcgatgcga ccagcggctc cttcgccttg gacgacccga
     1441 tcagacgaag agcatgtacg gccgccgacc gcatgtcctt gctctcgtgc ttgaggaacg
     1501 gcagcagctt cggtaccgcc ttggcctcgc ccacctcccc cagcgccacc accgcatacc
     1561 gctgcacccg catgttctcg tccttgagcg ccttcatcag cgccgggctc gcccgcgggt
     1621 ccctgagccg acccaacgcc tcggcggccc aacgacgcac cacgtagtcc ggagaagccc
     1681 ccagcgcctc gatcaaaggc tccaccgaag gcgcaccgat ggacaccagt gcgtcggctg
     1741 ccgcgcgccg cacgtccagg ctcggcgact ccaatgcgcc caacaggggc cccaccgcct
     1801 tggcgctccc aatcgccccc agcgactcgg ccgccgaccg tgccagatcc acattctcat
     1861 gctccagaaa gccgatcagt ggcccgatcg cccgcttcac cttcagctcg ccgagcgcca
     1921 cgacgatgga cttctggacc tccacgttcc gttccttgag gcgctcaatc agactatcga
     1981 ccaccgagtc gtcgccgatc ttccccaggg cctcggccgc ccacttccgg gccagcgaat
     2041 ccgtcgagtc gaggcacagc atgagcgcct cggtggcgca cgatcccagc ttcgccaacg
     2101 cctccgacgc gctccgctgc acgttgacac tggtgtcgcc cagggcctgc accaacggct
     2161 caaccgcccg gggatcacca atctcgccca acgcgcgggc cgaacacgaa cgcacctgat
     2221 acgcccgcgc gctcatcgcc tttagcagct cgggcacggc ccgttcgccc gccgcgatca
     2281 gctcctggac cgtctcctcc tgtaccgcct cgttcttgct cttcaggcga tccaccagct
     2341 cggcgacact ggccttccga ctcgccgtag ccatcggcgc gcctcctcct gccatggtta
     2401 gggatgttgc tcgaacggca aggccccaaa gactgcacta ctgcccatcg ctccgagccg
     2461 ccgcacgaac actgtgggcg agctggcgca ggtacgcctt ggcccccggg tcgcccccgc
     2521 gcatgagcgt ggccaaatca tccacgtccc gctgcaaggc ctggagccga aggcgcggag
     2581 gccccgccga caaccgctgt gcccgcgaca gcgccttcgc cgcactctcg accaggccca
     2641 gggcgccatc atccccaggc cccgaccagc gctgcgctgc tccttcgagt gcgtctgcgg
     2701 cctcggtcag ctccgaagcg cgagtccggg cccgaagcaa ccggtcatcc cgcgacctcg
     2761 cgttggccga ccggctctgg tacatggcaa cagtggtgag tgccgccacg aggagaagac
     2821 atgcagcgag gaacacacga gcatacccgt gccgatacag ccgcgctacc gctgctcgg
//
LOCUS       DAOSPL010000486         2878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_34701_length_2878_cov_2.194828, whole genome shotgun sequence.
ACCESSION   DAOSPL010000486 DAOSPL010000000
VERSION     DAOSPL010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34701_length_2878_cov_2.194828"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PLQ54_09655"
     CDS             complement(<1..492)
                     /locus_tag="PLQ54_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93565.1"
                     /translation="MRKEPYPSLTISWRIGVAHYETDRAFEDLLDQVRACREWLDELA
                     FFETLTHHLYLPLDALASRAAVLGRRIQAVRAEGIGRVGINILTTLGHINEAWDSMPA
                     LPFQPMVGHDGSVSRGCACPNTPGLREYVREKYSLFAEAGPDFIWVDDDIRMNNHGVA
                     WACF"
     gene            complement(705..>2878)
                     /locus_tag="PLQ54_09660"
     CDS             complement(705..>2878)
                     /locus_tag="PLQ54_09660"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOS93566.1"
                     /translation="VVYVMADLARRISDSRARRLAQSTFAVMDARGHDPDHGGYVERI
                     DLPIGDAANATKQLGTNMHVVLGLARLQMVSPSAVVRERLRELCDVLVAHALVPESGN
                     AYMAYTRDWRGRTSGEDPTQQTLYGHNAELVWYLLDAFDALGQDPSRYLPWLRRATDA
                     VIASGIGPDGRTHTWGPLIGRTADRDHARWWTPTEVLNMLARMYQLTSESRYLRLFDR
                     VARHTFGHFVTAQGQWYSDVNLADGSTADGSGDPWFAGLHVTRMLEECSKAISPGTPA
                     RHWPRTRMRPERAVQIEPGFGYYRDRSAESIVSEVVANGYDTIHLICLGRDPQPPGIV
                     PAAHARGVRIWATFFPSGVYMDDDLFPPERERWRMRFTSDALGAYRFFSYVHADYLEW
                     WKAHLRRIFDENEFDGLLFYEVHYPTLRDITGYGEVMFGDISPGFQDAFRRATGRGGF
                     PEFVDRASPRYYETDADLYCDYVDFRVNSIVRFLRDLLDGPGGVRREHPGLRFASWTI
                     AHSRPDALPILRENEAQDPARFVVELRPDLHYLQSHYPDWSNPELGPEYIHNYRPHMD
                     AAREGAPGLPIGVQVDVASTQPWRRDVQWMRGFDAVARELGLTSTTYYEFSLRWEVYF
                     DAPRVCEARWVGADEAEVVFDQRIDPASCTSFAQAKVDGNRLRFRVAGALRPGTEFSV
                     DVGGIADDPARRIPLGWDHDAGVGPANRIPPGTLVRLPAQR"
BASE COUNT          510 a         1063 c          846 g          459 t
ORIGIN      
        1 gaaacatgcc catgccaccc catggttgtt catccggata tcgtcatcca cccagatgaa
       61 atcaggaccc gcttcggcaa atagtgaata cttctcgcga acatactcgc ggagccctgg
      121 cgtgttcggg cacgcgcacc cccgcgacac cgatccgtcg tgtcccacca tcggctggaa
      181 cggcagggcg ggcatcgagt cccacgcctc gttgatgtgg cccagcgtgg tcaggatgtt
      241 aatgcccaca cgcccaatcc cctcggctcg caccgcctgg atccgccgcc ccaacaccgc
      301 cgcgcgactc gccagtgcgt ccagaggaag atacaggtgg tgcgtcagcg tctcgaagaa
      361 ggccagttcg tcaagccact cgcggcaagc gcgcacctga tccaacagat cctcgaacgc
      421 acggtcagtc tcgtaatgcg ccactccgat ccgccaggag atcgtcagcg aggggtaagg
      481 ctctttccgc atgcttggct cctgtcaaca ccaccgcggt cgctccgtgc cggccattcc
      541 ccgtccgcag cgggatccct tgcgcccaca gcaagaccga ccgccgagaa gcccgatctc
      601 acaagctccg ggaagcgaca ccctgttgta gtcgggtcgt ctgccgctgc gatggcgacc
      661 gtcaccccgg ggcggctcgc cggcagctgg cgtctccgtg acgctcaccg ttgcgccggg
      721 agcctcacga gtgtgcccgg gggaatccgg ttcgccggcc ccacgccggc gtcgtggtcc
      781 caacccaacg ggatgcgccg cgcggggtcg tccgcgatgc cacccacgtc aacagagaac
      841 tcggtgcccg gcctcagcgc gcccgccact cggaatcgca gtctgttgcc gtcgaccttc
      901 gcctgcgcga atgacgtgca gctcgccgga tcgatccgtt ggtcgaagac cacctccgcc
      961 tcatccgcac caacccaacg cgcctcacac acccgtggcg cgtcgaagta gacctcccac
     1021 cgtagcgaga actcatagta tgtcgtcgac gtgagcccca gctcccttgc cacggcatcg
     1081 aaaccgcgca tccactgcac gtctctgcgc cagggctgtg tcgacgccac gtcaacctga
     1141 acaccgatgg gcagcccagg cgcgccctcc cgcgctgcgt ccatgtgggg ccgatagttg
     1201 tggatgtact ccggccccag ctcagggttg ctccagtccg gatagtgcga ctgcaagtag
     1261 tgcagatccg gccgcagctc gacgacgaat cgcgcgggat cctgcgcctc gttctcccgc
     1321 aggatcggca gcgcatcggg ccgcgagtgt gcgatggtcc atgacgcaaa cctcaggccc
     1381 ggatgctccc gccgcacgcc gcccggcccg tcaagcaagt cgcgcaggaa ccgcacgatg
     1441 gagttcacgc gaaagtcgac gtagtcgcag tacaagtcgg cgtcggtctc gtagtaccgt
     1501 gggctcgccc ggtccacgaa ctccgggaac ccgccgcgcc ccgtcgcccg tcgaaatgca
     1561 tcctggaacc ccgggctgat gtcaccaaac atcacctccc cgtacccggt gatgtctcgg
     1621 agcgtcgggt aatgcacctc gtagaacagc agcccatcga actcgttctc gtcgaagatc
     1681 cggcgcaggt gtgccttcca ccactccaaa tagtcggcgt gtacatacga gaagaaccgg
     1741 tatgccccca gcgcgtcgct cgtgaaccgc atccgccacc gctcgcgctc cggaggaaac
     1801 aggtcgtcgt ccatgtagac gcccgacgga aagaacgtgg cccagatgcg tacccctcgg
     1861 gcgtgggcag ccggcacgat acctggtggc tgcgggtcgc gccccaggca gatcaggtgg
     1921 atcgtgtcat acccgttcgc cacgacctcc gacacaatgc tctccgccga ccggtcacgg
     1981 tagtagccga accccggctc gatctgcacg gcccgttccg gccgcatccg cgtgcgcggc
     2041 cagtgccgtg ccggcgtacc gggcgagatg gccttcgagc attcctcgag catccgcgtc
     2101 acgtgcaggc ccgcgaacca cggatcgccg ctcccgtcgg ccgtcgagcc atcggccaga
     2161 ttcacgtccg agtaccactg cccttgggcg gtcacgaagt gcccaaacgt gtgacgcgcc
     2221 acccggtcga acagccgaag ataccgcgac tcgctcgtca gctggtacat gcgcgccaac
     2281 atgttcagca cttcggtcgg cgtccaccag cgggcatggt ctcgatcggc cgtgcgaccg
     2341 atcaggggcc cccacgtgtg cgttcgtccg tcgggcccga tgcccgaagc gatcaccgca
     2401 tcggtagcgc gccgcagcca cggcagatag cggctcggat cctgcccgag ggcatcgaat
     2461 gcatcgagca ggtaccagac cagctcggcg ttgtgaccgt acagcgtctg ctgggtcgga
     2521 tcctcgcccg acgtcctccc gcgccagtcg cgtgtgtagg ccatgtaggc attgcccgac
     2581 tccggcacca acgcgtgcgc caccaacacg tcgcacagct cacgcaagcg ctcgcgcacc
     2641 acagccgacg gcgacaccat ctgcaaccgc gccagcccca gcaccacgtg catattcgtc
     2701 cccagctgct tcgtcgcgtt ggctgcatcc ccgatcggta gatcgatgcg ctccacataa
     2761 cccccgtggt ccgggtcgtg gccccgcgcg tccatgaccg cgaaggtgga ctgggccagc
     2821 cgcctcgccc ggctgtccga aatccgtcgg gccaggtccg ccatcacata cacgacgt
//
LOCUS       DAOSPL010000487         2877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_34711_length_2877_cov_3.210489, whole genome shotgun sequence.
ACCESSION   DAOSPL010000487 DAOSPL010000000
VERSION     DAOSPL010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34711_length_2877_cov_3.210489"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PLQ54_09665"
     CDS             complement(<1..461)
                     /locus_tag="PLQ54_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93567.1"
                     /translation="MVPVKHGVWGVVIYLCAATGLAAGGAADTEAGRGPELPKVGERI
                     QLSIRFGPDSETEAFDVIEIDPALVDRVRVPAYPGAVALRPVEDARQNKTPVMCLLTN
                     ENPDAVFAFYRRQDPTAEDITELVLQALLATSVAEGEPQTEPDKEALRDHFL"
     gene            complement(645..2204)
                     /locus_tag="PLQ54_09670"
     CDS             complement(645..2204)
                     /locus_tag="PLQ54_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOS93568.1"
                     /translation="MDSRTHNRVGAGLWISVLASLSATAWGAPADLLQAVAQVEPSVV
                     VVIASHEVGAAEPGKPTALRHMIGSGFFVSPTDIVTSARVLEGAQSVEITTASGERCG
                     AAIAGVDLPSRIAMLRVAEPVGTPVRFATGDVAKGQAIFTLGSYLGMGLTVTAGMVNG
                     TERTLTASGLPSVNDAFQIDAPVSADGAGSPVFAMDGSVVGMVLYSLVPEQGMLFRPP
                     DQGGGPVGEALMPSGRAEDAEGRATEPQRAIAAVVTFCIPSDTLEFVSRQFSADGRVK
                     RGRLGVQTRPVPAAVAAHLGVDPASSALVVSVAEGGPGALAGLKPYDVIVAMDGQPLR
                     GVRRLVKSVQMRPGQDVSLTVRRGADELRVTAHIDELVSEAPELGPGGVTTQVSAAGD
                     RPRAWLGVRAEPIDPSLAAHLGPAADGACVAYVFPGSPAEKAGLRRGDILVSIGPRLG
                     TDRAAAADAVARLWDRVASAAPGESVVLTVSRMDHGEQTVQLHASLAEMPAGLAIPPR
                     LTSVPPTGLVF"
     gene            complement(2319..2873)
                     /locus_tag="PLQ54_09675"
     CDS             complement(2319..2873)
                     /locus_tag="PLQ54_09675"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012899876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOS93569.1"
                     /translation="MFATQHGYAGIAATADGIVGSVCAEETAKAAARLLVWCAGNAVA
                     ANDRERLAAELATRGRASRGDSAKASMMVALGRKKLEDVAAGRRDLRGVPLDVSHRTP
                     FRQAVLREVAQIPPGTTLSYGEVAKRVGKPGAARAVGQTMAQNPLAPFVPCHRVVASN
                     GALHGFAGGLNAKAALLRAEGLDY"
BASE COUNT          442 a         1078 c          925 g          432 t
ORIGIN      
        1 aggaagtgat cccgcagagc ctccttgtca ggctcagtct gcggctcgcc ctcggccacc
       61 gatgtcgcga ggagggcctg gagaaccagt tcagtgatgt cctcggccgt agggtcctgt
      121 cgacggtaga aggcgaagac ggcatcgggg ttttcattgg tgagcaggca catcactggc
      181 gtcttgttct ggcgcgcgtc ctcgaccggg cggagcgcca ccgcgcccgg atatgcggga
      241 acacgcaccc gatcgaccag cgccggatcg atctcgatca catcgaacgc ctcggtctcg
      301 gaatccggtc cgaaccggat tgagagctgg atgcgctcgc cgaccttcgg cagctccggg
      361 ccgcggccgg cctccgtatc ggccgccccg cccgccgcga gccccgtggc cgcgcacagg
      421 tatatcacca cgccccagac cccatgcttc acgggcacca tgatgccagg cctccaccta
      481 tgcagacgat gtgctctatt cgggccttca cgtatggggc cctgcgagtg cgtgctcggc
      541 ccaaacgggg acagccacgg cctccggtgc atcggagacc cgtggctgtc acccgctcgt
      601 gccgcgctcc ctgctgccct atccccggcg gattccctat gccgctagaa caccagcccc
      661 gttggcggca ctgacgtcag cctgggcggg attgccagac ccgctggcat ctcagccagc
      721 gacgcgtgga gctgtaccgt ctgctcgccg tgatccatcc ggctcaccgt gagcactacg
      781 gactcccccg gcgccgccga ggccacgcga tcccacagcc tcgcgacggc atcggccgcc
      841 gcggcccgat ccgtccccag tcggggaccg atgctgacga ggatatcgcc gcggcgcagt
      901 ccggccttct cggccggcga ccccgggaac acataggcga cgcacgcgcc gtctgccgcc
      961 ggacccaggt gcgcggccag gctgggatcg atgggctctg cccggacgcc gagccacgcc
     1021 cggggccggt cgcctgccgc tgagacctgg gtcgtcaccc caccgggacc gagctccggc
     1081 gcctctgaaa ccagctcgtc gatgtgcgcg gtcacgcgta gctcgtccgc accccggcgt
     1141 accgtgaggc tcacatcctg gccgggccgc atctggaccg acttcaccag ccgccggacg
     1201 ccccgcaacg gttggccgtc catcgccacg atgacatcat agggtttgag cccggccagc
     1261 gccccggggc caccctcggc cacactgaca accagggccg acgaggccgg atcgacaccc
     1321 aggtgagctg ccacggccgc ggggaccggt cgcgtctgga cgccgagccg ccctcgcttc
     1381 acccggccgt cggccgagaa ctgccgggac acaaactcga gggtatcgct cgggatacag
     1441 aaggtcacca ccgcggcgat ggctcgctgc ggctccgtcg cgcgcccttc cgcgtcctcg
     1501 gcgcgtccgc tcggcatgag cgcctcgccg accggcccgc cgccctggtc cggcgggcgg
     1561 aacagcatcc cctgctcggg caccagtgag tagagcacca tccccacgac ggaaccatcc
     1621 atggcgaaca cgggtgatcc cgcaccatcg gcgctgacgg gcgcatcgat ctggaaggca
     1681 tcgttgaccg acggcaggcc gctggccgtc agtgtgcgct cggtgccgtt caccatcccg
     1741 gcggtgacgg tgaggcccat accgaggtag ctgccgaggg tgaagatggc ttgtcccttg
     1801 gcgacatcac cggtcgcgaa tctcaccggg gtgccgaccg gctcggcaac gcgcagcatg
     1861 gcgattcgag aagggaggtc gacgccggcg atggccgccc cgcagcgttc gccgctggcg
     1921 gtggtgatct cgacgctctg ggcgccctcg agcacccgcg cgctggtgac gatgtccgtc
     1981 gggctcacga agaagcccga gccgatcatg tgcctgagcg ccgtcggctt cccgggctcg
     2041 gccgcaccca cctcatgcga cgcgatgacc accacgaccg agggctctac ctgggccact
     2101 gcctgcaata ggtcggccgg ggcaccccac gcggtcgcgc tcaggcttgc cagaacggag
     2161 atccaaagcc ccgccccgac tcgattgtgg gtacgtgaat ccaccgcagt ccccatcctc
     2221 gcctcagtgc cacgtgcatc ctgagtggta gacgcctctg gggcgtggtg tggttctgga
     2281 atcttgagcg ccgggctccc gataagtcgc ggccggcctc agtagtccag cccctcggca
     2341 cggagcagtg ccgccttggc attcagccct ccggcgaagc cgtgcagagc gccgttcgac
     2401 gccaccaccc ggtggcacgg gacgaatggg gccagggggt tctgagccat ggtctgcccc
     2461 acggcccgcg ccgcgcccgg tttgcccacg cgcttggcga cttcgccata ggacagcgtg
     2521 gtcccgggcg gaatctgggc cacctcccgg agcactgcct gacgaaacgg cgttcgatgg
     2581 ctgacatcca gcggcacgcc tcggagatcc cgccggccgg ccgccacgtc ctcgagcttc
     2641 tttcgtccca gcgcgaccat catggacgcc ttggccgaat cgcctcggga cgcacggcca
     2701 cgggtcgcga gctctgccgc cagccgttcc cggtcgttcg ccgcaacagc attgcccgca
     2761 caccacacca gaagtcgagc cgcggccttg gctgtctcct cggcacacac actccccacg
     2821 atgccgtccg ccgttgcagc aatccccgcg taaccgtgct gggtggcaaa cacctcg
//
LOCUS       DAOSPL010000488         2871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_34798_length_2871_cov_3.214489, whole genome shotgun sequence.
ACCESSION   DAOSPL010000488 DAOSPL010000000
VERSION     DAOSPL010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34798_length_2871_cov_3.214489"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..327
                     /locus_tag="PLQ54_09680"
     CDS             7..327
                     /locus_tag="PLQ54_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOS93570.1"
                     /translation="MAEALGLTVLAGSACLDRPIHGALVSDLLSYVMASGRAGDVWIT
                     IQTHANTVAVAALASLAAIVVAAGFEPGDDTLSRAEEEEIPLLMSSQPAYVLAGRLFE
                     LGVR"
     gene            317..1099
                     /locus_tag="PLQ54_09685"
     CDS             317..1099
                     /locus_tag="PLQ54_09685"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOS93571.1"
                     /translation="MCGERMGADTLVAIDLHVHSALSPCGADEMRPPAVLLTAEQRGI
                     RVLGVVDHSTAGNARAFIEAAPAFDVRVLVGLEVESAEGVHTLALFDDWPPAMEMDRD
                     VAERLPALRNRPGLLGSQHYVDALGDTLGEEPRLLIAATDLTIEELADMAHERGGLSI
                     PAHVERQANGLLPTLGFVPQGLSVDAFELSRHTGRSDALRRWPDLSGRPVLWSSDAHC
                     LDDIGAGASWVPRGVAEASGPLRDWGPDLVRSVVRGPAGTDA"
     gene            1092..1640
                     /locus_tag="PLQ54_09690"
     CDS             1092..1640
                     /locus_tag="PLQ54_09690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS93572.1"
                     /translation="MREVSLHVLDLARNSIEAGASNVRVTVEESGPEDRLMVRVEDDG
                     RGMDAGTLERASDPFFTTRTTRRQGMGLSLFRSVCERCEGGMTLTSEPGGGTSVSGWM
                     RLSHLDRPPLGDVGAVVQALACEWPMVRARYEHRVDDRRFVLDLGELQAELGDVPVSS
                     PVVLSWIAEHVREQVACLGSTA"
     assembly_gap    1761..1770
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1771..2063
                     /locus_tag="PLQ54_09695"
     CDS             <1771..2063
                     /locus_tag="PLQ54_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOS93573.1"
                     /translation="VVIAMGTCGIAAGARDVMSALLEEMAQRGISDVTVSQTGCKGLC
                     DREPVVEVITPGAPSVTYGRVTPAVMRRIVAEQLVNGQIVSEYVIATGSEMR"
     gene            2067..2543
                     /locus_tag="PLQ54_09700"
     CDS             2067..2543
                     /locus_tag="PLQ54_09700"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOS93574.1"
                     /translation="MAVQTLCCETSNQIDAIIERRGADPDSLIEILHAVQEEIGYLPC
                     EVQEYVADRLGIAPGAVEGVVSFYSFFTTVPRGRHTVKVCQGTACYVRGGKRLLEAVS
                     KHCGCGVGETSADMRFSLDVVRCVGACGLSPVMTVGDDLYERVKPTRVAEILAKYE"
     gene            2577..>2871
                     /locus_tag="PLQ54_09705"
     CDS             2577..>2871
                     /locus_tag="PLQ54_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOS93575.1"
                     /translation="MRTYRSHVLVCAGASCISSGSQSVADRMEQTITECGLADEVRVI
                     RTGCMGSCDLGPVAVVYPEGVFYMKLTPDDAALVVQEHLLKGRPVERLMYHPDE"
BASE COUNT          461 a          866 c         1043 g          491 t
ORIGIN      
        1 cgccgcatgg ccgaggcact ggggctaacg gtgctcgccg gctctgcctg cctggaccgc
       61 ccgattcacg gggctctggt gagcgatctg ctcagctacg tcatggccag tggccgggca
      121 ggtgatgtat ggatcacgat ccagacccat gcgaacacgg tggccgtcgc ggcgcttgcc
      181 agtctggcgg ccattgtggt ggctgcgggt ttcgaaccgg gtgacgacac gctgtctcga
      241 gccgaggagg aagagatccc tctcctgatg tcgagccaac ccgcgtacgt cctggcggga
      301 cggctctttg agttgggtgt gcggtgagcg catgggcgcc gataccctcg tcgccatcga
      361 cttgcacgtg cacagcgcgc tgtcgccatg tggggccgac gagatgcgcc cgccagccgt
      421 gctgctgacg gcggagcaac gggggatccg agtgctggga gtcgtggacc acagtacggc
      481 cggcaatgcc cgggccttca tcgaggcggc ccccgccttt gatgttcgcg tcttggtcgg
      541 gctcgaggtt gagagcgccg agggggtcca cacgctggcg ctgttcgatg actggccgcc
      601 ggccatggag atggaccggg atgtggccga acgcttgccc gcgctgagga accggccggg
      661 cctgctcgga tcgcagcact acgtcgacgc actgggcgac actttgggag aggagccccg
      721 tctgctgatc gcggccacgg acctgacgat cgaggagttg gccgatatgg ctcacgaacg
      781 cggagggctg agcattccag cacatgtgga gcgccaagcc aacggactcc tgcccacgct
      841 cggcttcgtg ccgcagggcc tcagcgtcga tgcgttcgag ctgtcgcggc atacggggcg
      901 ctcggatgcc ctgcggcgat ggcccgacct gagtggccgg ccggtgctgt ggtcatcgga
      961 cgcgcactgc ctcgacgaca tcggggcggg cgcgagttgg gtgccacgcg gtgtggccga
     1021 ggcgtcgggc ccgttgcgcg attggggccc cgacctggtg cggtcagtcg tgcgagggcc
     1081 ggcgggcacg gatgcgtgag gtctcactgc acgttctgga tcttgcgaga aactccatcg
     1141 aggcgggcgc ttcgaacgtg cgcgtgaccg tcgaggagtc gggaccggag gaccgcctca
     1201 tggtgcgggt cgaggacgat ggccggggaa tggacgcagg cacgctggag cgggccagcg
     1261 acccgttctt caccacgcga acgactcggc gccagggcat ggggttgtcg ctgttccgat
     1321 cggtctgcga acgctgcgaa gggggtatga cgctgacttc ggagccaggt ggggggacca
     1381 gtgtgtcggg ctggatgcgc ctgtcacacc tcgaccgccc gcctctaggc gatgtgggag
     1441 cggtggtgca ggccctggcc tgcgagtggc cgatggtgcg ggcgcgctac gagcaccgcg
     1501 tggacgaccg ccgcttcgtt ctcgacctgg gggagctgca ggccgagttg ggcgatgtgc
     1561 ccgtcagctc gcctgtggtg ctgtcttgga tcgcggaaca cgtccgcgag caggttgcgt
     1621 gtctgggcag cacggcctga cgctttggcg ccgggccgga caacttcaga gggggcagga
     1681 gagcagtgaa gagccgagag gagctacggg ccatccgtga gcgcgccaaa gcaacgctgg
     1741 ccgtgcgcga caaccagggc nnnnnnnnnn gcgtcgtgat cgccatggga acctgcggca
     1801 tcgccgccgg cgcgcgggac gtgatgagcg ccctgctcga ggagatggcc caacgcggca
     1861 tcagcgacgt aaccgtatcg cagaccggct gcaagggctt gtgtgaccgc gagccggtcg
     1921 tcgaagtgat cacacctggt gcgccgtccg tcacctatgg gcgtgtgacg ccggctgtca
     1981 tgcgccgaat cgtggccgag cagctcgtca acggccagat cgtctcagag tatgtcatcg
     2041 ccaccggatc ggagatgcgc tgaagcatgg ctgtacagac cttgtgctgt gagacgtcga
     2101 accagattga cgccatcatt gagcgacgcg gggccgaccc cgactcgctg atcgagatcc
     2161 tgcacgccgt gcaagaggag atcggttacc tgccctgcga ggtgcaggag tacgtggcag
     2221 accgactggg gattgcgccg ggtgcggtcg agggcgtcgt cagcttctat agcttcttca
     2281 ccacagtccc tcgtggtcgg catacggtga aagtgtgcca gggaaccgcg tgctacgtcc
     2341 gaggaggcaa gcgcctgctc gaggccgtct ccaaacactg cggctgcgga gtcggcgaga
     2401 ccagtgcgga catgcgcttc agcctcgacg tcgtccgctg cgtgggcgcc tgtggccttt
     2461 cccccgtaat gaccgtgggc gatgacctgt acgagcgcgt gaagcccacg cgagtcgccg
     2521 agatcttggc caagtacgag tagctgacag ccatctagga aggtagagag ggtctgatgc
     2581 gcacttatcg ttcgcacgta ttggtctgcg ccggcgccag ctgcatctcc tcgggctccc
     2641 agtcggtggc ggaccggatg gaacagacca tcaccgaatg cggcctggcg gacgaagtca
     2701 gggtgatccg aacgggctgc atgggctcct gtgacctggg tccggtggct gtggtgtacc
     2761 ccgaaggcgt cttctacatg aaactgacgc ccgacgacgc cgcactggtc gtccaggaac
     2821 acttgctcaa ggggcgtccc gtggaacggc tcatgtacca ccccgatgag g
//
LOCUS       DAOSPL010000489         2870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_34819_length_2870_cov_2.715098, whole genome shotgun sequence.
ACCESSION   DAOSPL010000489 DAOSPL010000000
VERSION     DAOSPL010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34819_length_2870_cov_2.715098"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PLQ54_09710"
     CDS             complement(<1..886)
                     /locus_tag="PLQ54_09710"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HOS93576.1"
                     /translation="MRSGPWLMTMAFLTTLLSVPALADGILVPSHVRPAPDATPPAHF
                     SIRYHRVHLEITGQVLNCSVDQVFHNETRAELEGIYLFPAPPGATITKFSMDVDGKPT
                     EGRLLSRDEARSIYEGIVRKRKDPALLEYVDRTTFRARVFPIPPSGDKRIQISYTQLL
                     TVDNGLCEVVYPLDTERFSSKPIDEVTVVADIRPGAPLATVYSPSHELSIDKKADDHF
                     VASFEQTRVVPDTDFRLFYTLADQEFGLKVLTHRPRAGEDGYFLLMASPRRSAEVEER
                     TPKDVVFVLDTSGSMKTDG"
     gene            1070..1939
                     /locus_tag="PLQ54_09715"
     CDS             1070..1939
                     /locus_tag="PLQ54_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93577.1"
                     /translation="MSTEHDAQQSQTEPSSAAIAASPEQSRLHRFLLIVFLLAVSVAA
                     SASFLHSYQAEAGKEAALRAPAGIGAKSCGEPSQGCGASRHPGAANGAGKSCGAGPQA
                     IGATAREWSRDYGPTTAPTTLAYLVDPTQATDEDRSVAESLSRLAEGYAGKLRVVIRP
                     YGHPDARAYGGEHPAVLISERDSARKPGTLNSDAAPALRRDDSSCAAAAPRSCSAREM
                     KPGDRTRHGEARSVNGTDPVRPTAEASGYRVLFDTSAGSGGWTVEQLRDKVRAAIGPP
                     PTPSGSARTGAGA"
     gene            2129..2833
                     /locus_tag="PLQ54_09720"
     CDS             2129..2833
                     /locus_tag="PLQ54_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93578.1"
                     /translation="MDARGYRAQIAAGEGEPEPAVEVKRGIPLATVGVGILALAATAA
                     AAFFIMRFVSSEGQAAAGQASIGATSSATPGGPEAPSGEYGPADAPVQILAIPGHCEG
                     HQQMVKNLQALSKEYPKELHVIIASMGGPEASAEGRSCASYLMKVRGREEPGATPGGY
                     TLLAEKTPEQGWTPEGLTEQIRDTLNSVRKSSGGNAPAASEGGSKAEASKPPSGSDTP
                     KGSAKPAADPHAGNSH"
BASE COUNT          507 a          908 c         1009 g          446 t
ORIGIN      
        1 tgccatcggt cttcatgctg cccgaggtgt cgagcacgaa gacgacgtct ttgggtgtgc
       61 gctcctcgac ttcggcgctc cgccggggcg aggccataag aagaaagtaa ccgtcctcgc
      121 cagctctggg ccggtgcgtc agcaccttca gcccgaactc ctgatcggcg agggtgtaga
      181 agagccggaa gtcggtgtcg ggaaccaccc gggtctgctc gaagctcgcg acgaagtgat
      241 cgtccgcctt cttgtcgatc gacagctcat gggacggcga gtacaccgtg gccaggggcg
      301 cccccggccg gatgtcggcg accacggtga cctcgtcgat cggcttggac gagaagcgct
      361 cggtgtcgag cgggtagacg acctcacaga gcccattgtc aacggtgagg agctgcgtgt
      421 agctgatctg gatgcgcttg tcaccgctcg gagggatcgg gaacacccga gcccggaagg
      481 tggtccggtc gacgtactcg agcagcgccg ggtccttgcg cttccggacg atgccctcgt
      541 agatactgcg ggcctcgtct ctcgacagca gccggccctc agtgggcttg ccgtcgacat
      601 ccatcgagaa cttcgtgatc gtggcgcccg gcggagcggg gaacaggtag atgccctcga
      661 gctcggcacg ggtctcgttg tggaagacct ggtccacgct gcagttgagc acttggccgg
      721 tgatctccag gtgtacccgg tggtaacgga tgctgaagtg agctggcggc gtggcgtccg
      781 gagccgggcg tacgtgggag ggtaccagta tgccatcggc cagagccggg acggacaaca
      841 gcgtcgtcag gaatgccatg gtcatcagcc agggacctga gcgcatgggg agaccctcct
      901 cggtgtgccg ggtgcggtgc cggattagac cgggcgacgg ggccgagtgt tcccggcgtt
      961 catgccgcga attctctggg aatttcacca acaatatcgc cgttgaatgc agtgtccggc
     1021 ccagacccgg gcggtcgggc atatggctcc aacccggagg cgatgagtcg tgtcgacaga
     1081 gcacgatgcg cagcagagcc agaccgaacc ctcatccgca gccattgcgg cttccccaga
     1141 gcagagtcgg cttcaccggt ttctgctcat cgtgttcctt ctggccgtct cggtggcggc
     1201 atcggcgtcg ttcctgcact cgtaccaggc cgaagccggt aaagaggcgg cactcagagc
     1261 gccggccggt ataggcgcca agtcgtgtgg cgaacccagc caggggtgcg gcgcgagccg
     1321 gcaccccggg gccgcgaatg gtgcgggcaa gagctgcggc gccggtcctc aggccatcgg
     1381 tgccacggct cgcgaatggt cgagggacta tggccctacg acggccccga ccacactggc
     1441 ctacctggtt gacccgaccc aggccactga cgaagaccgc tccgtggcgg agagcctctc
     1501 ccgactggcc gagggctatg cgggcaagct ccgcgtggtg atacggccgt atggacaccc
     1561 ggatgcacga gcctatggcg gagagcatcc tgcggtgctg atctcggagc gtgactccgc
     1621 caggaagccg ggcacgctga actccgacgc cgcgcccgca ctccgtcgag acgactcctc
     1681 gtgtgcggca gcggcgccca gatcctgctc ggcaagagag atgaagccag gggaccggac
     1741 ccgccacggc gaggcgcggt cggtgaacgg gacggatccc gtccggccaa ctgcagaggc
     1801 gtcggggtac cgggttctgt tcgacacgag cgccgggagt gggggctgga cagttgaaca
     1861 gcttcgcgac aaggtgcgcg ccgccatcgg gcctccgccc acaccaagcg gctcagcccg
     1921 gaccggtgcc ggggcgtagt cccacgtcgt ggccggacga cctcggggca gggcttcggt
     1981 ggtgccagtg cctcctcaac aagcgccttg gattgcggca ggccagttgc cacccgggcc
     2041 gatatggcgg agaatcggct ggagaggatt cccggcttcg gcacctaaag ccggttcagc
     2101 aagccgaaac actatggagg cggcagtcat ggatgcgcgt ggatatcgtg ctcagatcgc
     2161 ggccggagaa ggcgagcccg agcctgctgt ggaggtgaaa cgcggcatcc cgttggccac
     2221 cgtcggcgtg ggcattctgg ccctggctgc caccgctgcc gcggccttct tcatcatgcg
     2281 gttcgtgtcg agcgagggcc aggcggccgc gggccaggca tcgatcggag ccacaagctc
     2341 ggccactccc ggtggtcccg aagccccgag tggcgagtat gggccggccg atgcgccggt
     2401 gcagatcctg gccattcccg gccactgtga gggccatcag cagatggtca agaacctcca
     2461 ggcgctgagc aaggagtacc cgaaggagtt gcacgtcatc atcgcctcga tgggcggccc
     2521 ggaagcgtct gccgaaggcc gcagctgcgc ctcgtacctg atgaaggtca gggggcgcga
     2581 agaaccggga gccacaccgg gcggctacac gctgctggcc gagaagacgc cagagcaggg
     2641 atggacccca gaggggctga ctgagcagat ccgagacaca ctcaactccg tacgcaagtc
     2701 gagtgggggg aatgcgccgg ctgcctccga gggcggcagc aaggccgaag cgagcaagcc
     2761 gcccagcggc tccgatacgc ccaagggaag cgccaagccc gcggctgacc cccacgccgg
     2821 caactcgcac tagatcgatg gcgctgagac cactctctgt gtgtcgtatg
//
LOCUS       DAOSPL010000490         2869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_34827_length_2869_cov_3.516347, whole genome shotgun sequence.
ACCESSION   DAOSPL010000490 DAOSPL010000000
VERSION     DAOSPL010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34827_length_2869_cov_3.516347"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            346..1122
                     /locus_tag="PLQ54_09725"
     CDS             346..1122
                     /locus_tag="PLQ54_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013324595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOS93579.1"
                     /translation="MLRSCSRRLVVYVGLLLSAFTLVLVAGCKSGEEGTTPAEPGGMK
                     KAVSQAEPPAKTTAKENATEEGAPELKVGTTLENLQAAFDGESNAHKRYLAFAAKAEE
                     EGYKQAALLFTAAARAEEIHAKSHAKVIEKLGGTPTADIKEPEVKTTKENLEAALKGE
                     TYEKNTMYPEFLKVAKADKNADAIRTLNGAMTVEEEHAALYQAALDDLEGQKAITSPL
                     WVCPVCGHTVQAQKLDFSNCPICNTPADQFEKIDQVAAAK"
     gene            1205..1693
                     /locus_tag="PLQ54_09730"
     CDS             1205..1693
                     /locus_tag="PLQ54_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93580.1"
                     /translation="MPISLPPVPKWEGLHPLVVHFPIALLIVAPLFVATGMLLWERTR
                     AYAVAALILMVLGTAGAWVSVASGVAAMGLATVNAEGREVMARHQELAMLVRLAFSIL
                     TALYAAIVALPLFAKRLTPKVVVPVHLVFFVLYAGAVLVLAAQSHLGGRLVHEFGVQA
                     LM"
     gene            2258..>2869
                     /locus_tag="PLQ54_09735"
     CDS             2258..>2869
                     /locus_tag="PLQ54_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006345750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93581.1"
                     /translation="MRRSWLMFMASILVAGCNGHGADGNNGNNNDNGGDPNTITVSPG
                     ESIQAAVDQAQPGQTVLIRPGTYRPSGAAEALVVIRAAKDGITIRGGGATPGDVVIDG
                     NDQVLHVFFFDQGIGRSTTIENLTVTGGRAYPEDVFPAGFTSVLRPELNQDGDFYHDG
                     AGVMLFMCAPTVRNCRIEGNEASRCGGGISVFNPGDGAFPNPGP"
BASE COUNT          504 a          938 c          972 g          455 t
ORIGIN      
        1 ctcgagaggg aatatgaccc gcagatcgtc accacgatgc accgggcgct gaaggacggc
       61 tcctacacgg acgagttgtt cgagaagcgc acaggcaagc cgctcgacga attgtgggag
      121 gactacaagg cccggtgggg cggcgagtag cggagccagt gcagcgggcg tccggcgtcg
      181 ggaaccagca acaggtgtgc ggacgctgag tattcggagg ctcaccctga ggccacgcgg
      241 gttgggtttg cctcggctgg gagcgacatc tctcagcccg cagagagccg ggggcccctg
      301 gtggttgcct cggctccgcc aagtactgca tggagggcgt cgccgatgct ccgttcctgt
      361 tcacgccgtt tggtggtgta cgtaggcctg ttgctttccg cattcacgct cgtgctggta
      421 gccgggtgca agtcaggcga ggagggcacg acgccggccg aaccgggtgg catgaaaaag
      481 gctgtcagcc aggccgagcc acccgccaag accaccgcca aagagaacgc aaccgaagag
      541 ggagcgcctg agctgaaggt cggcaccacg ctcgagaacc tccaggccgc tttcgacggc
      601 gagagcaatg cccacaagcg ctacctcgct ttcgcggcca aagccgaaga agagggctac
      661 aagcaggccg ctctgctctt caccgcggcg gcccgggccg aggaaattca cgccaagagc
      721 cacgccaagg tcatcgagaa gcttgggggc acgccgaccg ctgacatcaa ggaacccgag
      781 gtgaagacga ccaaggagaa cctcgaggcg gctctcaagg gcgaaaccta tgagaaaaac
      841 actatgtacc ccgagttcct aaaggtcgcc aaagccgaca agaacgccga cgccatacgg
      901 acgctcaacg gcgccatgac ggtcgaggag gagcacgcgg cattgtacca ggcggccctg
      961 gatgacctgg agggccagaa ggcgatcacg tccccgttgt gggtctgccc cgtctgcggc
     1021 cacacggtcc aggcgcagaa gctcgacttc tccaactgcc ccatctgcaa cacgccggct
     1081 gaccagttcg aaaagatcga ccaggtagcg gcggccaagt agcgaaggcc tgccacagac
     1141 tgtcgcggcg actggcggcc ccgcctggca gcggggctgc cttcccttcg cagaggagac
     1201 caacgtgcca atctcgctgc cgcccgtacc caagtgggag ggcctccacc ccctggttgt
     1261 gcacttcccc attgccctgc tgatcgtcgc cccgctcttc gtggctaccg ggatgctgct
     1321 ttgggagcgg acgcgagcct acgccgtggc ggcgctgatc ctgatggtcc tgggtacggc
     1381 gggggcatgg gtgtccgtgg cctccggcgt cgctgccatg gggttggcca cggtcaacgc
     1441 cgaggggcgt gaggtgatgg cgcgccatca ggagctggcg atgctcgttc gcttggcctt
     1501 cagcatcctg accgcgctct acgcggccat cgtcgcgctg cccctgttcg ccaagcgcct
     1561 gacgccgaag gtggtcgttc ccgtgcacct cgtgtttttc gtgctctatg cgggcgcagt
     1621 gctggtgctc gcggcccaga gccacctggg tgggcggctg gtgcacgagt tcggggtgca
     1681 ggcgctgatg tgagggcgcg gcccctcccc tggtgcctct caaccagtgc cgtcgcggcg
     1741 aggtgggtca cggctcccgg tggcacggcc ctgcgcgtca tggggcaatg gtccgccaga
     1801 ttgcggagga atggctcact tcgggtcgcc tcggtccatg gcacgtactc ggatggggca
     1861 tcgtaaggga gcagtgcgaa gcccgtctgg acgacgcgct aatcgcgctg aggcttcgct
     1921 tgcctgccta gccaggatcc ggcccgtgcc gagcggtcgg ccctgcctca ccccgcagcg
     1981 tcggcgtcgc ctggccggac aggccggacc tgccctctgc tccgcagcct gacgcagctg
     2041 gcatattccg cgcaccttcc tacagagccc gcccgtaccg cgcgtgccat gccagggaaa
     2101 ctcgcagacc tcacaacgca tcatcgtcac gacaccaggt agtggggctc aggcgtcgaa
     2161 ctctgacggc ggcgcccaca tcggtgggac ccggagcgcc tcgggcgcgc cgatcccctg
     2221 gccggtgaac gcgggccggc gttgcggagg gttaccaatg cgacggtcgt ggctgatgtt
     2281 catggcaagc attctggttg cgggctgcaa cggccacggt gccgatggca acaatggcaa
     2341 caacaacgac aacggcggcg acccgaacac catcacggtg agccctgggg agtccatcca
     2401 agcagcagtg gatcaggcgc agcccgggca gaccgtgctt atccggccgg gtacctatcg
     2461 cccatccggc gccgcagaag ctctggtggt catccgcgcg gccaaagatg gcatcacaat
     2521 ccgcggtggc ggtgccaccc cgggcgacgt ggtgattgac ggcaacgacc aggtgctcca
     2581 cgtgttcttc ttcgatcaag ggatcggccg gagcaccacg atcgagaacc ttacggtcac
     2641 ggggggccgg gcctaccctg aggacgtgtt tcccgcgggg ttcacgtcgg tgctccgccc
     2701 cgagctcaac caggacggcg acttctacca cgatggggcg ggcgtcatgc tgttcatgtg
     2761 cgcccccacg gtccgcaact gccggatcga gggcaacgag gcctcgcgat gtgggggcgg
     2821 catctcggtg ttcaacccgg gagacggcgc gttcccgaac ccgggtccg
//
LOCUS       DAOSPL010000491         2867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_34862_length_2867_cov_3.329659, whole genome shotgun sequence.
ACCESSION   DAOSPL010000491 DAOSPL010000000
VERSION     DAOSPL010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34862_length_2867_cov_3.329659"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PLQ54_09740"
     CDS             complement(<1..629)
                     /locus_tag="PLQ54_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS93582.1"
                     /translation="MQPAIELTGVRKTYRVRGAAPVPAIDGVTLAVQPGEVFGFLGRN
                     GAGKTTTVKVLLGMVPQYEGSARLFGIDARRPAARFRTGYLAETPEFAPHATVREILS
                     LHARLAGMKRGAGEDRAEELIDLVDLRGAVGRRVGGFSKGMIQRLGVAVALIGEPRLV
                     FLDEPTANLDPVGRRMVRDVLLGLRSQGVTVFLSSHMLSEVEMTCTRVA"
     gene            complement(660..2426)
                     /gene="bamD"
                     /locus_tag="PLQ54_09745"
     CDS             complement(660..2426)
                     /gene="bamD"
                     /locus_tag="PLQ54_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOS93583.1"
                     /translation="MPRAWTILFSVAAIGIGGALLYANRSDAHGEDPWVQAERSFSAG
                     DLRAAARQYEAFVARHPDSPWAAQALYQAAFIYADDARRRDDAIRTYRRIADEFPHHP
                     LADEALRHMGDLLLEDHQYAEAIRAYQDAVHRSAGSDPAASAAAEVRIAEALFRSGDG
                     PGARLRCLAILRRYPRCGHQRAEALNLLALTALQLEDSPRTALQRYEQLLREYPDSRY
                     APQARRQVTALRQELGLPAVTADRTEATRGRSLRVELPTRSPSGEAFKDTGMLECLRV
                     LLAASGVHVSLTKLSGWSGRAFAFYYAATDRRRGAGLMPPGEPEGLARSLGFRHCALT
                     IERSPERAWDRVVRSLSDGQAVLTRAGVVSPADWVLITGYDAATDRVGFHTTSGARRT
                     VPLDQLLGALWAPEGGPGMGARSDDGSREIPLFVLGRRPASSQPAPSLAPAIRQAIAL
                     GRAHDDWGLRGGLSAWTELAHDMARVGGSGCTDAEAAELGAWCESDLPRLTASRREAA
                     DFLRTWAASATPVARKHLESAARHYDGVARHLAGLEAAFRAALEHRPSASQLARPEVV
                     VTRAAAEERAALRELEAVVEAP"
     gene            complement(2554..>2867)
                     /locus_tag="PLQ54_09750"
     CDS             complement(2554..>2867)
                     /locus_tag="PLQ54_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93584.1"
                     /translation="VPILLSVALIPQAIDDQALLSRPYVVQSARAARDLREAELYLDE
                     AANVGHDPVQLAQIGRRVDAALRAGLADRIAALAADAPDDDQRALVQIIDDLKAIARC
                     E"
BASE COUNT          401 a          998 c         1096 g          372 t
ORIGIN      
        1 atggcgacgc gcgtgcaggt catctcgact tcggagagca tgtgggagct caggaagacg
       61 gtgacgccct ggctgcggag cccgagtagc acgtcccgca ccatgcgccg acccacgggg
      121 tcgaggttgg ccgtgggctc gtcgaggaac acgagcctcg gctcgccgat gagggccacg
      181 gccacgccca ggcgctggat catccccttc gagaaccccc cgacgcgtcg gcccacggcc
      241 ccgcggaggt ccaccaggtc gatgagctcc tcggcccggt cttcgcccgc cccccgcttc
      301 atgccggcca ggcgggcgtg gaggctcagt atctcgcgaa cggtggcgtg gggggcgaac
      361 tcgggggtct cggcgagata gccggtgcga aagcgggccg ccggccggcg ggcatcgatg
      421 ccgaacaggc gggccgagcc ttcgtactgg gggaccatgc ccaggagcac cttgacggta
      481 gtggtcttgc cggctccgtt gcggcccagg aacccgaaca cctctccggg ctgaacggcc
      541 agggtgacgc cgtcgatggc cgggacgggc gccgctccgc gcacgcggta ggtcttccgg
      601 acgccggtga gctcgatggc aggctgcacg cggtcggccc tccccgggcg gcatgttgcc
      661 tatggcgcct cgacgacggc ttcgagctcg cgcagggccg cgcgctcctc ggccgcggcg
      721 cgcgtcacca ccacctcggg gcgcgcgagt tggctggcgc tcgggcgatg ctcgagggcc
      781 gcgcggaatg ccgcctcgag tcccgccagg tggcgcgcca cgccgtcgta gtggcgggcc
      841 gccgactcca ggtgcttccg agcgaccggt gttgccgatg cggcccacgt tcgcaggaag
      901 tcggccgcct cgcgccgcga ggccgtcagc cggggcagat cggactcgca ccaggcaccc
      961 agttccgcgg cctcggcatc ggtgcagccc gagccaccga cccgggccat gtcgtgggcg
     1021 agctcggtcc acgccgagag acccccgcgg aggccccagt cgtcgtgggc tcggcccagc
     1081 gcgatggcct ggcggatcgc gggagccagc gagggcgcgg gctgggacga cgccggccgg
     1141 cggcccagga cgaagagcgg gatctcgcgc gagccgtcat cggaccgggc gcccatcccg
     1201 ggcccgccct cgggggccca gagggccccg agcagttggt cgagcggcac cgtccgccgg
     1261 gcccccgatg tggtgtggaa gcccacgcgg tccgtggccg cgtcgtagcc cgtgatgagc
     1321 acccagtcgg caggtgagac gacgcccgca cgcgtgagta ctgcctggcc gtcggacagg
     1381 cttcggacca cgcggtccca cgcacgctcg gggctgcgct cgatggtgag ggcacagtgg
     1441 cggaaaccca ggcttcgcgc caggccctcg ggctcccccg gtggcatgag accggcgccg
     1501 cggcggcgat ccgtggccgc gtagtagaac gcgaaagcac ggcccgacca gccggacagt
     1561 ttcgtgagcg agacgtgcac accgctcgcc gcgaggagca cgcgcaggca ttcgagcatc
     1621 ccggtgtcct tgaacgcctc gcccgaaggc gagcgggtcg gcagctccac ccgcagggag
     1681 cggccccgtg tggcctcggt ccggtctgcc gtgaccgcgg gcaagcccag ctcctgccgg
     1741 agggcggtga cctggcgtcg ggcttgcggc gcatagcgcg agtcggggta ctcgcgcaga
     1801 agctgctcgt agcgctggag ggccgtgcgc gggctgtctt cgagctggag cgcggtgagg
     1861 gcgagcaggt tcagggcttc ggcccgctgg tgaccgcagc gcgggtagcg ccggaggatg
     1921 gccaggcagc gcagccgggc cccggggccg tcgccggagc gaaacagggc ctcggcgatc
     1981 cggacctcgg cggcggcgga cgccgcgggg tccgagccgg cactgcgatg gacggcatcc
     2041 tggtacgcgc gaatcgcttc ggcgtactgg tggtcctcga gcaggaggtc tcccatgtga
     2101 cggagggcct cgtcggccaa ggggtggtgc gggaactcgt cggcgatgcg ccggtaggtg
     2161 cggatggcgt cgtcgcgccg ccgggcgtca tcggcataga tgaacgcagc ctgatagaga
     2221 gcctgtgcgg cccaggggct gtcgggatgg cgggcgacga acgcctcgta ctggcgcgcg
     2281 gccgcccgaa ggtcgcctgc cgagaaggac cgctcggcct ggacccaggg atcttcgcca
     2341 tgggcatcgg accggttggc atagagaaga gcgccgccga tgccgatggc cgcaaccgag
     2401 aacaaaatgg tccacgcacg tggcacgcgg gcctcacctg ccgccagggc tggggggcgc
     2461 gagccccccc acactcgtcc gccagaccga acgagccgca cggcccaggg caccggccgg
     2521 cggcccgcag tctggcctca cacgcgcttg tgtctactca caacgcgcga tcgctttcag
     2581 gtcgtcgatg atctggacca gcgctctctg gtcgtcgtcc ggtgcatcgg cggcgagcgc
     2641 cgcgatccgg tccgccagcc cggctcgcag ggcggcgtcg acccggcggc cgatctgggc
     2701 gagctggacc gggtcgtgcc ccacgttggc cgcctcatcc aggtacagtt cggcctcgcg
     2761 cagatcgcgc gcggctcggg cggattggac cacatagggg cgagagagca gcgcctggtc
     2821 gtcgatcgcc tgggggatga gcgccacgct gagcaggatc gggaccg
//
LOCUS       DAOSPL010000492         2864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_34911_length_2864_cov_3.364899, whole genome shotgun sequence.
ACCESSION   DAOSPL010000492 DAOSPL010000000
VERSION     DAOSPL010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34911_length_2864_cov_3.364899"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PLQ54_09755"
     CDS             <1..572
                     /locus_tag="PLQ54_09755"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOS93585.1"
                     /translation="PDALEPYYSQQTVELHHGKHHAAYVKGLNEAEAKIAEMAQSGDF
                     GQAKATCKALAFHGSGHVLHSVFWTNMRPGGGGDPQGPLAYALSLEFRSVDAAKSLFV
                     AATNAVEGSGWGVLAYVPETNRLVILQAEKHENLTQWGAIPLLVCDVWEHAYYLQYQN
                     KRADWVAAFMEHLVNWDDVAARFEAARRR"
     gene            complement(569..1405)
                     /locus_tag="PLQ54_09760"
     CDS             complement(569..1405)
                     /locus_tag="PLQ54_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93586.1"
                     /translation="MRTGLLTTIVLLVALLLSACGAERAATGEANGLTDMQQLVGERV
                     ANAASTTYLLGQMVAPALNALQSTQAKASADEAPALQGLLENALGDGAVQVDREPDSL
                     LYSVTVSNGSIFGGRVPVSGTLTSSAVLWDPFSLSVSSEGLTVGKASVSGALALCLPV
                     NDPALAITAHDVRIAQEDGGAACRFDLDATVTPKSLGAVAVNGTMVVTAEGLTRAGDV
                     RVTVVAEDLTARVASPYPSSGTVTFVCEEEELRITFDGSRWAKLSDGAGHWVAVPLPA
                     LI"
     gene            complement(1617..2489)
                     /locus_tag="PLQ54_09765"
     CDS             complement(1617..2489)
                     /locus_tag="PLQ54_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93587.1"
                     /translation="MQTRVPLALSLLSLAVTLAACGNTSPVDAGGGNTGDGTAGLSVI
                     QQDVADRVAAADQAAAALTQVLGPTADAAVAAQSRAIGASARSMQAADEVLERFLGTG
                     SVEIGLQLSPPGYLITVTNGKLFAGTVPVNGALTVTGTMATDPRIITVATSDFTVGQC
                     TLSGTILVTVPAAGGSITMAATDLSVAWHGAAVVSEFSLNATATATDGGGYVIDGNLV
                     VSIEDVGGAESAEVTVTARGVESGPATAYPCAGSLAFTCCGERLEIVFTGGSTATVSD
                     REGHSATLALPALV"
     gene            complement(2617..>2864)
                     /locus_tag="PLQ54_09770"
     CDS             complement(2617..>2864)
                     /locus_tag="PLQ54_09770"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HOS93588.1"
                     /translation="QVSGGGVRGLALGDRPPAVAALCSIQVQIAELAVEAAVEGSRQA
                     ALHALLIDPVINDIDVAGKILDDYLTVHADYLPQFAR"
BASE COUNT          465 a          964 c          988 g          447 t
ORIGIN      
        1 ctcccgatgc gctggagccc tactacagcc agcagacggt cgagctccac cacggcaagc
       61 accacgcggc atatgtcaaa gggctgaacg aggccgaggc caagatcgcc gaaatggccc
      121 agtcgggcga cttcggccag gccaaggcta cgtgcaaggc cctagcgttc cacggttcag
      181 ggcacgtgct gcactcggtc ttctggacca acatgaggcc cggaggcggg ggcgacccgc
      241 aggggccact ggcctatgcg ctttcactcg agttccgcag cgtggatgcg gccaagagcc
      301 tgttcgtggc tgccaccaat gcggtggagg gctccggttg gggcgttctg gcttatgtgc
      361 ccgagaccaa tcgcctggtg atccttcagg ccgagaagca cgaaaacctg acccagtggg
      421 gcgccattcc cctgctcgtc tgcgatgtgt gggagcacgc ctactacctg cagtatcaga
      481 acaagcgagc cgactgggtc gcggcgttca tggagcactt ggtgaactgg gatgatgtgg
      541 ccgcccgctt tgaggcggcg cgacgccgct agatcagcgc gggcaggggc acggcgaccc
      601 agtgccccgc accgtcggac agcttcgccc agcggctacc atcgaaggtg atgcggagct
      661 cttcctcttc gcacacaaag gtgaccgtgc cgctggacgg gtacgggctg gccacgcgag
      721 ccgtaaggtc ctcagccacc accgtgaccc gcacgtcgcc ggcccgggtg aggccctcgg
      781 ccgtgacgac catggtgccg ttgacggcta cggcacccag gctcttgggg gtcacggtgg
      841 cgtcgaggtc gaacctgcag gctgcccctc catcctcctg ggcgatgcgc acgtcgtgag
      901 ccgtgatcgc cagggcggga tcgttgacgg gcaggcagag ggccagggcg ccgcttacag
      961 aagccttacc gaccgtgagc ccctcactgc tgaccgagag cgagaacggg tcccacagaa
     1021 cggccgagga ggtgagcgtc ccgctcaccg gtactcgtcc accgaagatg gagccattgc
     1081 tcacggtcac cgagtagagg agcgaatccg gctcgcgatc gacctgcacg gccccgtccc
     1141 cgagggcgtt ctcgagcagc ccctgcagcg ccggtgcctc gtcagcgctc gccttggcct
     1201 gcgtgctctg gagcgcgtta agggccggcg ccaccatctg gccgaggagg tacgtcgtgc
     1261 tggcggcgtt ggcgacgcgc tcgccaacca gctgctgcat atcggtcagg ccgttcgcct
     1321 cgcctgtggc cgcccgttcc gccccgcacg cgctcaacag cagtgccacc agaagcacaa
     1381 tggtcgtcag taggccggtg cgcatgggcc agctttctcc gttcccgatt ctcgtgagtc
     1441 ctgcgatgtt atttcgttgg gtggtacgca acacctcctg ccgcaacgcc tggggccgtc
     1501 tccgggttcg tcgggcaggt ggcggcccca agatgcagtg cggggcgggc ccgagaccgg
     1561 gccttgcccc ggacgcgata gatggtgccc cgaaccgtga caggcgacag ggcgggtcag
     1621 accaatgccg gcagggcgag ggtagccgag tgcccttcac ggtcggacac ggtcgccgtg
     1681 ctgccaccgg tgaacacgat ctccagtcgc tcgccacaac aggtgaatgc cagcgagccc
     1741 gcgcaagggt aggcggtcgc cgggccggac tcgacgcccc gggccgtgac ggtcacctcg
     1801 gccgactcgg cgccgccgac gtcctcgatg ctgacgacga ggttgccgtc gatgacatag
     1861 cccccgccgt ccgttgccgt cgccgtggcg ttcagactga actccgatac cacagccgcg
     1921 ccgtgccagg ccacggacag gtcagtggcc gccatggtga tgctcccgcc ggcggcggga
     1981 acggtcacca agatggtgcc actcagggtg cactgtccca cggtgaagtc gctcgtggcc
     2041 actgtgatga tcctcgggtc ggtcgccatg gtgcccgtga ccgtgagagc tccgttcacc
     2101 gggacggtgc ccgcgaagag cttgccgttg gtcaccgtga tcaggtagcc gggtgggctg
     2161 agttgcaggc cgatctcgac cgagccggtc cccagaaacc gctcgagcac ctcatcggcc
     2221 gcctgcatgc tccgggcgga tgcgccgatg gcccggctct gggccgcgac cgccgcatcg
     2281 gcagtgggcc cgagcacttg ggtgagcgcg gcggcggcct gatcagccgc ggcaacgcga
     2341 tccgccacgt cctgctggat gaccgagagc cccgcagtcc cgtcgccggt gttgcccccg
     2401 ccggcgtcga cggggctcgt gttgccgcag gcagcaagcg tcacggcgag tgagagcagg
     2461 ctgagagcca gcggtacacg cgtctgcatt gcggtaatcc ccctgagccg aggaggctgc
     2521 ctcggcgcac atagtctagc gtcggtatgc cacacccggg gcgacgcggg atgcggcatg
     2581 tgcggggatt cgtcggagac gggggcgcac ggcgcgctac cgggcgaact ggggcaggta
     2641 gtcggcgtga accgtcaggt agtcatcgag gatcttgccg gcaacgtcga tgtcgttgat
     2701 gactggatca atcaacagcg cgtgcagcgc agcttgccgg ctcccctcga ccgcagcctc
     2761 tacagccagt tcggcgatct gcacctggat gctgcacagg gcggccacgg cgggaggacg
     2821 atcgccgagc gcaagcccgc gcacgccacc cccactgacc tggg
//
LOCUS       DAOSPL010000493         2861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_34967_length_2861_cov_2.779401, whole genome shotgun sequence.
ACCESSION   DAOSPL010000493 DAOSPL010000000
VERSION     DAOSPL010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34967_length_2861_cov_2.779401"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1503
                     /gene="aspS"
                     /locus_tag="PLQ54_09775"
     CDS             <1..1503
                     /gene="aspS"
                     /locus_tag="PLQ54_09775"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOS93589.1"
                     /translation="RIGTGDVELVAHAVTILNEAKTPPFPVEDDADIGEDVRLRYRYI
                     DLRRPRMSEMLHTRHRFILETRKYLDSQGFWEIETPMLLSNTPEGARDFIVPSRMNPG
                     RFYALPQSPQLLKQTLMVAGVDKYFQIARCLRDEDLRADRQLEFTQIDIEMSFVEQDD
                     VLEMTEGLMAHLFRTIAGYDPPRPFPRLTYAEAMSRYGTDKPDLRYGMPIVDVSDIAA
                     RTEFQVFRGVVSGGGTVRCIRVEGGAAFSRGQLDGLQARAQEFGGKGAAWIVFAEGAI
                     KGPLGKFFPAELEAELRQRTGSRDGDLLVFSADTEKTVCMVLGRMRTHLAAQLGLVKK
                     GVFHLSWVTDFPLFEFDDELGHYVPMHHPFSMPQPGDIEKLDTDPGAVMGQLYDLILN
                     GNEAASGSIRIHRRDVQSKVFEVIRVSEADARRRFGFLMEAFEYGAPPHGGIAPGLDR
                     IVAEILGRESIRDVIAFPKTAAGTDLMVGAPNEVPQEQLDELHIRVVERE"
     gene            1633..2430
                     /locus_tag="PLQ54_09780"
     CDS             1633..2430
                     /locus_tag="PLQ54_09780"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOS93590.1"
                     /translation="MPRRSHDSGFIRLNKLLAGKGVASRRHVEEMIIAGRIRVDGELA
                     TEKGLKVRPNAQIEVDGHVVGQPPPLAWVALHKPRGYVCTKEDSRDRPIVMDLLPPEL
                     SHLHPVGRLDGDVSGLLLFTNEGELTHRLTHPRYGIEKVYHCHTTSPMRPRQAQALEE
                     GIELEDGPSYPAQCRVLRPSGSGSLVELRIHEGRKHQVKRMFEAVGNPLIQLERVAVG
                     PVELGSLGRGRYRPLTPYEVIELHKRTMLPIPKGLGERSGVQAAPAS"
BASE COUNT          472 a          942 c         1007 g          440 t
ORIGIN      
        1 aggatcggca cgggtgacgt ggagttggtg gcgcacgccg tcaccatcct caatgaagcg
       61 aagaccccgc cattccctgt ggaagacgat gcggacattg gcgaggacgt tcggctgcgc
      121 taccgctaca tcgatctccg tcgacctcgc atgtccgaga tgctccacac gcggcaccgg
      181 ttcatcctgg agacccgcaa gtacctggac tcgcagggct tctgggagat cgagaccccc
      241 atgctcctga gcaacacgcc cgagggcgct cgcgacttca ttgtgcccag ccggatgaac
      301 ccgggccgct tctacgcgct accccagtcg ccgcagcttc tgaagcagac gctcatggtg
      361 gccggcgtgg acaagtactt ccagatcgcg cggtgtctgc gcgatgagga ccttcgggcg
      421 gatcgccagc tcgagttcac gcagatcgac atcgagatgt cgttcgtgga acaggacgac
      481 gttctcgaga tgaccgaggg tctgatggcc catctgttcc ggaccattgc cggctacgat
      541 ccgccgcggc cattcccgcg cttgacgtac gctgaagcca tgtcgcgtta cggcaccgac
      601 aagccggacc tgcgctacgg catgccgatt gtcgatgtgt ccgatatcgc ggcccgcacc
      661 gagtttcagg tcttccgcgg tgtcgtgtcc ggcgggggca cagtgcggtg tatccgggtg
      721 gagggcggcg ctgcgttctc ccggggccag ctggatggcc tgcaggcgag ggcgcaggag
      781 tttggaggaa agggcgcggc gtggattgtc ttcgccgagg gcgccatcaa ggggccgctg
      841 ggcaagttct tcccggccga gctcgaggcc gagctgcgcc agcggaccgg cagccgcgat
      901 ggcgatttgc tcgtcttctc tgccgacacc gagaagaccg tctgcatggt gctgggtcgg
      961 atgcggaccc atctcgccgc ccagctgggg ctggtgaaga agggcgtgtt ccacctttcg
     1021 tgggtcaccg acttcccgct ctttgagttc gacgacgagc tgggccacta tgtcccgatg
     1081 catcatccct tctcgatgcc gcagccgggc gatatcgaga agctggacac ggacccgggg
     1141 gcggtgatgg ggcagctgta cgacttgatc ctcaatggca acgaggccgc aagtgggagc
     1201 atccgtatcc atcgccgcga cgtacaatcg aaggtgttcg aggtcattcg cgtgagcgag
     1261 gcggacgccc gtcgccggtt tgggttcttg atggaggcgt tcgagtacgg ggctccgccc
     1321 catggtggaa ttgcgccggg cctcgaccgc atcgtcgccg agatcctcgg gcgtgagagc
     1381 attcgcgacg tcatcgcgtt cccgaagacg gccgccggta cggacctcat ggttggagca
     1441 cccaacgagg tcccccagga gcagctcgac gaactgcaca tccgggtggt ggaacgcgag
     1501 tagcgacgcc cggccggtgc cggacgggcc gagcgctcgg cctcccgtgg gccgtggggg
     1561 cgatctcggc tcccgtgcgg ggcgcccgga gcgcttctcc ggcggcgcga ccacggacgc
     1621 gggggatgat gcatgccacg ccgctcgcac gacagtgggt tcatacgcct gaacaagctc
     1681 ctggccggca aaggcgtggc ttcgcgacgc catgtcgagg agatgatcat cgccggccgc
     1741 atccgggtcg acggcgagct ggccacggag aagggcctca aggtccgccc gaacgcccag
     1801 atcgaggtgg acggacacgt ggtgggccag ccgccgcctc tggcctgggt ggccctgcac
     1861 aagccccggg ggtatgtatg caccaaggag gactcccgcg atcgacccat cgtcatggac
     1921 ctccttccgc ccgagctttc gcacctccat ccggtgggcc gactcgacgg cgacgtctcg
     1981 gggctcctgc tgttcacaaa cgagggggaa ctgacgcacc ggctgaccca cccacggtat
     2041 gggatcgaga aggtctacca ctgccacacc acgtcaccga tgaggccgcg gcaagcacag
     2101 gcactggagg aggggattga gctcgaggac ggtccgtcct atccggccca gtgccgggtc
     2161 ctgcgcccca gtggctcggg ctcgttggtg gagctacgca tccacgaagg ccgcaagcac
     2221 caggtgaagc gcatgttcga ggccgttggc aatcccctga tccagctgga gcgcgtggcc
     2281 gtcgggcccg tcgagctggg cagtctgggg cgcggacgct acaggcccct gacaccgtat
     2341 gaggtgatcg agctgcacaa gcgcaccatg ctcccgatcc ccaagggcct gggggagcgt
     2401 tccggcgtcc aggccgcacc ggcatcttag ggggagggcg attcgaagtc cccgacctcg
     2461 aggagaccga gaggcgggac tcgagccgct cggcccgcgc gggccgagcg ggcgccagat
     2521 gggggcatcg agtcgcccga gaagccggcg accccgtcac ggaaaccgcg tccgccgcgg
     2581 cggagagctg agccgcagcg gctctcgaag ccctcggctg accggcccga acgccgcgcg
     2641 aagcacgccc cgcaggacca ggcaccggct ggcgagcgct ccggcaagcg cggggcggga
     2701 cggcctcgcg ggacgcgccc gcccggcgac gaccggttgc ccggtctgcg aggacgccag
     2761 ggcccgtctg catggggtgt ccaggagggc atcggaccga cgcggcagcc cccggagcgc
     2821 ccaccggctt ggggcggcgc cgagaagccc gcgggacgac c
//
LOCUS       DAOSPL010000494         2858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35014_length_2858_cov_3.581163, whole genome shotgun sequence.
ACCESSION   DAOSPL010000494 DAOSPL010000000
VERSION     DAOSPL010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35014_length_2858_cov_3.581163"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLQ54_09785"
     CDS             <1..194
                     /locus_tag="PLQ54_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93591.1"
                     /translation="GAVRGGAAGWCLHNGSPRGGAAGPARSFDLRPGQPRLMEQLDEV
                     ELDVLRRMANTAAEARAGR"
     gene            216..1574
                     /locus_tag="PLQ54_09790"
     CDS             216..1574
                     /locus_tag="PLQ54_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008180959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOS93592.1"
                     /translation="MIGTIAMAMALASAAGELTVAAPAVTTISLPFSASEESQGGLVA
                     ADVDADGAMDLVVTRPGALAAVRVDGTMLWVREADIVVGGSSEAEGLPGHHGPGVQAG
                     DIDGDGSAEVVFLTRDGVLHVCSGATGEDRWTARPPVPNGAERWEHAIVCCLRGVGDR
                     DILLQATNQDGYRMGRYLAAYSAEGLREGEAPLWTLDDFPACAHNGARVADLDGDGRD
                     EVLSAAIVGPDGAMLCHPPIRGHVDSVFVDDVRPDLPGLEVVLLEEGGEDGNRVFLLS
                     RDRVIWEAHCDHQEPQNAAIGRFDGERPGAQVWCRSRYDTHQRPFVFDSDGTLIRQYA
                     MDDVAPEGWTEKGVEVIHTITWSGERQLACAKERHESGEICLFDPISGQFLWWSEGER
                     ADRLYVADVLGDWREEMVVWSGSQLRIYANPEPDPEPGHSRLWDEPRYRRSKMTWNYY
                     SP"
     gene            1646..2713
                     /locus_tag="PLQ54_09795"
     CDS             1646..2713
                     /locus_tag="PLQ54_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93593.1"
                     /translation="MAVAQPAETGRHREPGASRHTEVSIRGDAFYINGRPTYEGRTWQ
                     GHRIEGLLLNSRMVQGIFDDLNPDTRGLFAYPDTGVYDAERNTREFIAAMPEWRRHGL
                     LAFTINLQGGLPRAGASSQPWHNSALTESGDLRPEYMARLEAILDRADELGMVAIVGI
                     FYFGQDQRLKDEAAVVRGLDGAVDWVLGRGYRHVLIEVNNECNVRYDHAILQPARVHE
                     LIERVKSRERDGRRLLVSTSYGGGTIPGENVVRAADFVLLHGNGVGDPAGIAEMVRAT
                     RRVPGYRPMPIVFNEDDHYDFGQPANHMTAAIGEYASWGYYDQGAADYTSGYQSPPVK
                     WGISPGRNRAFFDQVREITGG"
BASE COUNT          420 a          853 c         1131 g          454 t
ORIGIN      
        1 ccggggccgt ccggggtggg gcggcggggt ggtgcctgca caatggttcg ccgcgcgggg
       61 gtgccgcggg gccggcgcgg tccttcgacc tgcgacccgg tcagcctcgg ctcatggagc
      121 agctggacga ggtcgagctg gatgtgctca ggcggatggc gaacacggcc gccgaggccc
      181 gggccggccg ctgagacagg agacggagga tcgacatgat aggcaccatt gcgatggcca
      241 tggcgctggc ctcggcggcg ggggagctca ccgtcgccgc gccggcggtg acgaccatct
      301 cgctgccgtt ctcggccagt gaggagtcgc agggtggcct tgtggctgcg gatgtggatg
      361 cggacggggc gatggacctg gtcgtgacac ggccgggcgc cctggcggcg gtccgcgtgg
      421 acggcacgat gctgtgggtt cgtgaggcgg acatcgtggt ggggggcagt tccgaggcgg
      481 agggactccc cggccaccat ggtccgggtg tccaggccgg cgatatcgac ggcgacgggt
      541 cggccgaagt ggtgtttctc acgcgtgacg gcgtgctcca cgtgtgctcg ggagcgacgg
      601 gggaggaccg gtggacggcg cggccgccgg tccccaatgg cgctgagcgc tgggagcacg
      661 ccatcgtgtg ctgcctacgg ggcgtgggcg atcgggatat cctgctgcag gccaccaacc
      721 aggacggtta ccgcatggga cggtatctgg ccgcctactc ggcggagggg ctccgggagg
      781 gcgaggcgcc tctgtggacc ctcgatgact tccccgcctg cgcccacaat ggcgcccgtg
      841 tggcggacct ggatggtgac ggccgggacg aggtgctctc ggcggccatt gtggggccgg
      901 atggggcgat gctgtgccac ccgccgatcc gcggccatgt ggactcggtg ttcgtcgatg
      961 acgttcggcc ggatctgcct ggcctggagg tggtgctgct cgaggagggc ggcgaggacg
     1021 gcaatcgcgt gttcctgctt tcgcgggacc gtgttatctg ggaggcgcac tgcgaccacc
     1081 aggagccgca gaatgcggcg atcggacgtt tcgatgggga gcggccgggg gcccaggtgt
     1141 ggtgccggag tcggtatgac acccaccagc gaccgttcgt gttcgactcg gacgggactc
     1201 tgattcggca gtatgccatg gatgatgtgg cgccagaggg ctggacggag aagggcgttg
     1261 aggtcattca caccatcacg tggtcgggtg agcgccagct ggcctgcgcc aaggagcgtc
     1321 acgagtctgg tgagatctgc ctgttcgacc cgatctcggg ccagttcctg tggtggtcgg
     1381 agggcgagcg ggcggaccgg ctctatgtgg ccgacgtgct gggtgactgg cgggaggaga
     1441 tggtggtctg gagtggttcg cagctgcgga tctacgccaa cccggagccc gaccctgagc
     1501 ccgggcactc gcggttgtgg gatgagccgc ggtaccgccg cagcaagatg acgtggaact
     1561 actacagccc ctaggcgcgg cgggatgtcc gagtgatgga tccagggggg tgttggcctt
     1621 ggttgcgacg atcgctcttg tggccctggc ggtggcccag ccggccgaga cgggccgcca
     1681 tcgggagcca ggcgcctcac gccacaccga ggtctcgatc cgcggcgacg ccttctatat
     1741 caacggccgg cccacgtatg aggggcgcac gtggcaaggg caccgcatcg aggggcttct
     1801 gctgaactcg cggatggttc agggcatctt cgatgacctg aaccccgaca cgcgaggcct
     1861 gttcgcatat ccggatacgg gtgtctacga cgcggagcgc aacacgcggg agttcatcgc
     1921 ggccatgccc gagtggcgcc ggcacggctt gctggcgttc accatcaacc tccagggcgg
     1981 gctgccacgg gcgggcgcgt cgagtcagcc ttggcataac tcggcgctca ccgagagcgg
     2041 cgatctgcgg cccgagtaca tggcgcggct cgaggccatt ctcgaccggg ccgatgagct
     2101 gggcatggtg gctatcgtgg gcatcttcta cttcgggcag gaccagcggc tgaaagacga
     2161 ggcggccgtg gtgcgcgggc tcgatggcgc ggtcgactgg gtgctgggcc ggggctaccg
     2221 ccatgtgctg atcgaagtca acaacgagtg caatgtgcgg tatgaccacg cgatcctgca
     2281 gccagcccgc gtgcacgagc tgattgagcg ggtgaagtcg cgggagcgcg acgggcggcg
     2341 gctgctggtg agcaccagct acggcggggg gacgatcccg ggggagaacg tggtccgcgc
     2401 ggccgacttc gttctgctgc atggcaacgg ggtgggggac ccggcgggca tcgccgagat
     2461 ggtgagagcc acgcgccggg tgcccggtta ccggccgatg ccgatcgtgt tcaacgagga
     2521 cgaccactac gacttcggcc agccggcgaa ccacatgacg gcggccatcg gcgagtacgc
     2581 ctcgtggggc tactatgacc agggggcggc cgactacacc agcggatacc aatcgccacc
     2641 cgtgaagtgg ggcattagcc cgggccggaa ccgggcgttc ttcgaccagg tgcgggagat
     2701 cacgggcggg tagaggggaa ggccggcgcg agggtacccg ggccggcccg tccacaagac
     2761 ggggcagtgc ggtgtgcggg ccttgcgcgt cccccccccg gggcattggc ggtaacttgg
     2821 ggctcccgtg gcccatccgc ctggttgcgg aggggcgc
//
LOCUS       DAOSPL010000495         2853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35102_length_2853_cov_2.852752, whole genome shotgun sequence.
ACCESSION   DAOSPL010000495 DAOSPL010000000
VERSION     DAOSPL010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35102_length_2853_cov_2.852752"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PLQ54_09800"
     CDS             complement(<1..498)
                     /locus_tag="PLQ54_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009182657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HOS93594.1"
                     /translation="MDKADTGFVLLSSGLVMLMTPGLALFYGGMVRIKNVLSTLLQNF
                     FMLGLMSIIWAVIGYSLAFGEDLGGGLLGGLNHVFLAGVGSELKDGLTIPTNVFMIFQ
                     AMFAIITPALFTGAIAERTKFGALMLFGGLWSIVVYSPVAHWVWGPDGWLAGMHALDF
                     AGGTVV"
     gene            complement(536..874)
                     /locus_tag="PLQ54_09805"
     CDS             complement(536..874)
                     /locus_tag="PLQ54_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOS93595.1"
                     /translation="MVRIEAVIRPSKLDDVRQALSEAGVVGMSVADIRGCGRQRGFTE
                     RYRGSEYTINLLHKVRVEVVVPDDKAEGAIETIAAAARTGEVGDGKIFVIPVADAMRI
                     RTGERGADAL"
     gene            complement(1494..2033)
                     /locus_tag="PLQ54_09810"
     CDS             complement(1494..2033)
                     /locus_tag="PLQ54_09810"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOS93596.1"
                     /translation="MRLVAFSPQARALLLCTAIAPLLCLALCCGRGGADRAPIEPPDS
                     VDPSITADEWGSIHAFPSVKHGLGVASADRRPTMVYFHSDANTNCLRVVEELFTDPGV
                     AMLAGHFVTVVVDVTEDKETAKAYGIDSFPSFVFLDHKGRVIRRVKGLKRSAEFRNEM
                     TEAENAFKDALAAESAGER"
     gene            complement(2228..>2853)
                     /locus_tag="PLQ54_09815"
     CDS             complement(2228..>2853)
                     /locus_tag="PLQ54_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93597.1"
                     /translation="LALREYSGAVRTGLIRRLPDTWITFDREAHCRGYSRVLDDHRDE
                     YARALDQGRKPGARTVATGTTSASVPDEAVGGTPWTGRNPHKYLNAEFCSVQVRESGG
                     LWREVEADGRVEVPRGRRIELRVAVANTGEPRWLADGNGAVRLRVEAPGGPHYADLQG
                     DVDRYGRVAFAPIALGPSYTETTVSVRMEVRGRGGFGQKMTVVLRPR"
BASE COUNT          471 a          983 c          950 g          449 t
ORIGIN      
        1 cacgacggtg ccgcccgcga agtcgagggc gtgcatgccc gcgagccagc cgtccggccc
       61 ccagacccag tgggccacgg gggagtacac cacgatcgac cacagaccac cgaacagcat
      121 caacgcgccg aacttcgtgc gctcggcgat ggcgccggtg aacagcgccg gcgtgatgat
      181 ggcgaacatg gcctggaaga tcatgaacac gttggtggga attgtcaggc catctttgag
      241 ctccgacccg acaccagcca ggaagacgtg attcagtccg cccagtaggc caccgcccag
      301 atcctcgccg aacgcgagtg aatagccgat gacggcccag atgatgctca tgagaccgag
      361 catgaagaag ttctggagga gcgtgctgag tacgttcttg atccgcacca tccccccata
      421 gaagagcgcc aggccgggtg tcatcagcat gacgagcccg gaactcaaca gcacgaagcc
      481 cgtgtctgcc ttatcaatgc ccattgcttc tgcctcctct ccgccccccg ggggctcata
      541 gggcgtcggc gccgcgctct ccggtccgga tgcgcatggc atccgcaacg ggaatgacga
      601 agatcttccc atcgccgacc tcgccggtcc gggccgccgc agcgatggtc tctatcgcac
      661 cctcggcctt gtcgtccgga acgacaacct caacgcggac cttatggagc aggttgatgg
      721 tgtactccga tccgcgatag cgctcggtga agcccctctg ccggccgcac ccgcggatgt
      781 cggcgacgct catgccgacg acacccgcct cgctgagcgc ctggcgcacg tcgtcgagct
      841 tactgggccg gatcacagcc tcaatgcgaa ccatcggtgc atccctcctg accgggccgc
      901 cgtgggcggc cgtgccgccg ggactggcac ccgtgcccca gtcccggcag tcatttaccg
      961 gcaatggcta tccggtaagt atatgccgat ggtagcggcg catcgggtgg ctgtcaagtg
     1021 ctcattgctc gggaaaatgg gattcctccc ccaatcgggc ccaggtgatt gggtgtatcg
     1081 cgtcctagga tagggcactg aatgccggta tattgccgat agtggaacac cattcgaact
     1141 cacatgaggg tatgttcgac cggtatccag ggacctgtcg gaggcatgtg tcgggaccca
     1201 caagatgaga cggccgtggc ggtcgccacc acagtggcag tctgtgcggc gcggcctgag
     1261 acccgcgcta cgggtcctgt cgccctttgg caggctcagg gctggtgggg ccggggccta
     1321 ttggcgacca cccagcccgg cggcggggcg gctcggcctg gctcagcgcc catgagccga
     1381 gggggctggg tccggtcgat gggacggggc cccgccgcgg tggctccagg ccctccctcc
     1441 aacgcaccca gcggacgcct gggctgcgcg aggtgaccgc tcgtgggcgc gagtcatctc
     1501 tcgccggccg actcggcggc gagggcgtcc ttgaacgcgt tctccgcctc ggtcatctcg
     1561 ttgcggaact cggcggaacg cttgaggccc ttgacccgcc ggatgactcg acccttgtgg
     1621 tccaggaaga cgaagctggg gaagctgtcg atgccgtagg ccttggcggt ctccttgtct
     1681 tcagtgacat cgacgacgac cgtgacgaaa tggcccgcga gcatggcaac cccgggatcg
     1741 gtgaacaact cctcaaccac ccgcaggcag ttggtattgg cgtccgagtg gaagtacacc
     1801 atggtgggcc ggcggtcggc gctggcgacg cccagaccat gcttgacgct ggggaacgcg
     1861 tggatgctgc cccattcgtc ggccgtgatg ctcggatcga cgctgtcggg gggttcgatg
     1921 ggcgcccgat cggcgccccc gcgtccgcag cagagcgcga ggcacaggag aggggcgata
     1981 gccgtgcaga gcaagagagc gcgggcctgc ggcgagaagg cgaccagtcg catgggcgaa
     2041 gctcccttcg gggggccggc gacgcgtctc ggcccaccag cgtaggacac cccgacctcg
     2101 ctcccgcacc cccaagccac ccccgggccg cgcaggcccc tgctgttcga cgcccggcag
     2161 agccggatgt cgcgcccgtg cccctggctc aggtgccctg agcacccagc gggttgggcg
     2221 aggcgagcta tcgaggtcgc agaaccactg tcatcttctg cccgaatccg ccgcgccctc
     2281 tcacctccat gcgcaccgag accgtcgtct ccgtgtagga aggccccagg gcgatgggcg
     2341 cgaaggccac tcggccatac cgatcgacgt cgccttgcag gtcggcgtaa tgcgggcctc
     2401 ccggcgcctc gactcggagc ctgacggcgc cattgccgtc ggccagccag cggggctcgc
     2461 ccgtgttcgc caccgccaca cgcagctcga tccggcgccc ccgcggcacc tcgactcggc
     2521 cgtcggcctc tacctcgcgc cacagcccgc cggattcgcg cacctgcacg gagcagaact
     2581 cggcgttgag gtacttgtgc ggattccgcc cggtccaggg cgtgccgccg acagcttcat
     2641 caggcacgct ggccgacgtg gtacccgtcg ccaccgtacg agcccccggc ttgcgcccct
     2701 ggtcgagcgc gcgggcatac tcgtcccggt ggtcgtcgag gactcgcgaa tagccgcggc
     2761 agtgggcttc gcggtcgaaa gtaatccacg tgtcggggag gcgccggatc aggccggtac
     2821 ggaccgcgcc cgaatactcg cgcagggcga ggg
//
LOCUS       DAOSPL010000496         2848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35165_length_2848_cov_3.147870, whole genome shotgun sequence.
ACCESSION   DAOSPL010000496 DAOSPL010000000
VERSION     DAOSPL010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35165_length_2848_cov_3.147870"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PLQ54_09820"
     CDS             complement(<1..697)
                     /locus_tag="PLQ54_09820"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOS93598.1"
                     /translation="MGDQPPDGPARGGPRWPVVAAMAVAVAGVAVVVVAAWPRDSGMR
                     VRWAVSTFGPVVAPPLLAGDRLFAASTDGTVRCLDRDSGQELWVSTAPRLAVRCGMAL
                     AGGTLYCCSDANLVVALSASDGSVRWEHETRGPMQSAPVVYDGRVYAAGDDGILRVLS
                     AHTGVLERYYRRPSYVAADLAVSSNGVLAMGCGDGFLHLIDLTQPAVRALNGEWRPEE
                     KRAEAQAAIHGDPV"
     gene            complement(704..1729)
                     /locus_tag="PLQ54_09825"
     CDS             complement(704..1729)
                     /locus_tag="PLQ54_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93599.1"
                     /translation="MLSERAEQVLAVHQDRLAGMYRGDNPGHVFSIAGLPLGYDTGPA
                     ALEAPRQWVSRCVDDLGRPMALVEDAVTFRPPVLEPGTHGVHFVDALFGAEVFAGAGQ
                     VWSRELVGDLVDLSCPDPSESPLAVKAIECGRTSLEATAGRVWLGTVVLASPLNVGVN
                     LWGERLLAALINEPESAVTGLRTVTDTIAGLHRAYRQAVPPDTLRFYAASSRFAPAGF
                     GHICGCTTQLVSRAVYEEHVAPLDREILGLYPEGGSIHLCGAHEHLIPVFRDMEELRA
                     VQLNDRASDGFPHYYAGLRDDQLIYVTPSEQMPLDRILAISGGHRVIIQSCIGERIGL
                     RKPLEAE"
     gene            complement(1790..>2848)
                     /locus_tag="PLQ54_09830"
     CDS             complement(1790..>2848)
                     /locus_tag="PLQ54_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOS93600.1"
                     /translation="IMAGGWGQRLWPLSTPSRPKPLLGLGPGPSLLADAVGHARLLAS
                     VDHVFIAASQDVAEAIRGAQTGVPDRQVLVEPGKRDTLGCLAYVLARLEADELARPAD
                     AVLALLTADHVIRDVEAFRGTALRACRLARDTGRPALIGVSPTRPEVGFGYLEVGPSA
                     GPGYATVRRFHEKPSPDQAETYLRHGQFLWNSGMLFCRVGVLRDRVRERVAAVDMAIA
                     RMVEAWRSRSVEAVAEAFGCIPAMSIDYALLEGWDELLVVPGEFGWDNINGWEAVHRL
                     YPADEAGNVVRGSAALLDCHDCLVIHEGAAGALGITVVGMEAAVVVVASGHVLVCPRG
                     REFRLGQGGGSVRQGGGH"
BASE COUNT          521 a         1082 c          868 g          377 t
ORIGIN      
        1 ccaccgggtc cccgtgaatg gctgcctggg cctcggccct cttctcctcc ggcctccatt
       61 cgccgttcag ggcgcggaca gccggttggg tcaggtcgat gaggtggagg aaaccatcgc
      121 cgcaccccat ggccagaacc ccgttggacg acacggccag atcggccgct acataggacg
      181 gacgccggta gtaccgctcc aggacacccg tgtgggccga cagcacccgc agtattccat
      241 catcgcccgc cgcgtagacg cggccgtcat acacaaccgg tgccgactgc atgggaccgc
      301 gcgtctcgtg ctcccatcgg accgaaccat cagaggcgga gagcgccacc accaggttgg
      361 catcgctaca gcaatagagg gtgccaccgg ccagcgccat cccgcagcgc acggccaggc
      421 gcggcgccgt gctcacccag agctcctggc ccgagtcacg atcgaggcag cgcaccgttc
      481 catccgtcga tgccgcaaag agccggtcgc cggccagcaa cggaggagcc accaccgggc
      541 caaaggtgct gaccgcccac cgcacccgca tacccgagtc acgcggccag gccgcgacga
      601 caaccaccgc gacccccgcc acggccacgg ccatcgccgc cactaccggc caacgcgggc
      661 cgcctcgagc cgggccgtct ggtggctgat cgcccatacg gccctactcc gcctccaagg
      721 gcttcctcag accaatccgc tcgccgatgc acgactggat gatgacccgg tgacctcctg
      781 agatggccag gatgcgatcc agcggcatct gctcactcgg cgtcacgtag atcagctgat
      841 cgtcacgcag cccggcgtag tagtgcggga acccatcgga cgcacggtcg ttgagctgca
      901 cggctcgaag ctcctccata tcgcgaaaca ctggaatcag atgctcgtgc gccccacaga
      961 ggtgaatgga gcccccctcc gggtacaggc cgaggatctc gcgatcaagc ggcgccacat
     1021 gctcctcata gacggcacgc gagaccaact gcgtcgtgca tccacagatg tggccgaagc
     1081 ccgcaggggc gaaacgcgac gaggccgcgt agaaccgcaa cgtgtctggc ggcacggctt
     1141 gccggtaagc gcgatgcagc ccggcgatcg tgtccgtgac cgtccttagc cccgtgaccg
     1201 ccgactcggg ttcattgatg agggctgcca gaagccgctc gccccagagg ttcaccccca
     1261 cattcagtgg actcgccagc accaccgtgc ccaaccacac tcggccggcc gtcgcctcga
     1321 ggctggtgcg cccgcactcg atcgccttca ccgccagcgg ggactcactc ggatcggggc
     1381 agctcaaatc caccagatcc cccacaagct cgcgcgacca gacctgcccc gcaccggcga
     1441 acacctcggc gccgaacagg gcgtcgacga agtggactcc atgggtcccc ggctcaagca
     1501 ctggcgggcg gaatgtgacc gcatcttcga ccagtgccat cgggcggccg aggtcgtcga
     1561 cgcatcggct aacccactgc cgcggtgcct ccagcgccgc gggcccggtg tcgtaaccca
     1621 gcggcagtcc ggcgatgctg aacacatggc ccgggttgtc gccgcggtac atgcccgcca
     1681 gtcggtcctg atggaccgcc aacacctgct cggctcgttc cgacagcaat ccggcgcctc
     1741 ccgcagcccc gcccaacaac ggctcggcac actgtaccgg ccggtcgcat cagtgtccac
     1801 cgccctgccg cacggaccct ccgccttggc ccaagcggaa ctcgcgcccc cgggggcaga
     1861 cgaggacgtg cccgctggca accaccacca cggccgcctc cataccgacc acggtgatcc
     1921 ccaacgcgcc agcggccccc tcgtggatca caaggcagtc atggcaatcg agaagggccg
     1981 cgctgccgcg gacgacgttg ccggcctcat cggctgggta caaccgatgc accgcttccc
     2041 aaccgttgat gttgtcccat ccaaactcgc cgggcactac cagcagctcg tcccagccct
     2101 ccaggagcgc gtagtcgatc gacatggccg gtatgcaccc aaacgcctcc gcgacggcct
     2161 caacggaccg gctgcgccag gcctctacca tccgggcaat ggccatatcc acggctgcga
     2221 cccgttcgcg gacgcgatca cgcagcacgc cgacccggca gaagagcatc cccgagttcc
     2281 acaggaactg gccgtgacgg aggtaggtct ccgcctgatc tggcgatggc ttctcgtgga
     2341 accgccgcac cgtcgcatag ccgggtccgg cgcttggccc cacctccaga taaccaaacc
     2401 ccacctcggg ccgggtcggc gacacaccaa taagcgccgg tcgtcccgtg tcgcgggcga
     2461 gcctgcaggc acggagtgcc gtacctcgga acgcctcgac atcgcggatg acgtggtcag
     2521 cggtcaggag cgccagcacc gcgtctgccg gccgcgcgag ttcgtcggct tcgagccggg
     2581 caagcacata ggccaggcag ccgagcgtgt cgcgcttccc gggctccaca aggacctgcc
     2641 gatcgggcac gccggtctga gccccacgga tcgcctcagc cacatcctga ctggccgcga
     2701 tgaagacatg atccaccgag gccagcaggc gcgcgtgacc cacggcgtcg gccaacagcg
     2761 acggaccggg accgaggccc aacaagggct tgggacgcga cggcgtggac aggggccaca
     2821 ggcgctgacc ccacccgccg gccatgat
//
LOCUS       DAOSPL010000497         2840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35303_length_2840_cov_2.498743, whole genome shotgun sequence.
ACCESSION   DAOSPL010000497 DAOSPL010000000
VERSION     DAOSPL010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35303_length_2840_cov_2.498743"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PLQ54_09835"
     CDS             complement(<1..751)
                     /locus_tag="PLQ54_09835"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOS93601.1"
                     /translation="MKTMQAERIFGPMDMRVVEVPIPELGDHDVLCRVVRSGVCGTDY
                     AIYTGEFRFVKNGGITFPMTPGHEWSGVVADVGSAVERFAPGDRVVGDTGVACGHCNE
                     CLVGNYGVCAKARAVGTINAWPGAYAEYVVMPERHVFPLPDSVSFDNGAMVEPAATAL
                     FSVVKAGVSIGDTVLVQGSGPIGIAAAKLAKLSGASKVLITGRKDFKLQKALELGVDA
                     AVNTQKESIAEAVLRHTKEKQVDRVIEAAGSV"
     gene            complement(748..1770)
                     /locus_tag="PLQ54_09840"
     CDS             complement(748..1770)
                     /locus_tag="PLQ54_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HOS93602.1"
                     /translation="MAGRWTIARLNAETAPITEVERHILAAVDPCIVEAEGASDDEVR
                     SAARDCDALMVVSAHVRGSVIEQLTRCRIISRLGTGVDKIDVEAATRRGIFVTNLPDF
                     CTNEVADHTMALLLAAARQLKLFEAGARQGKVPRDVRHMRRLATCTLSLIGFGRIGRA
                     VAVRARAFGMRVLACDPALTAEAAAETGVSVVDLPTALAEADYLSLLCPLTPATRHML
                     TLEQFRQMKPTAVLINTGRGELVDEDDLVTALREGIIRYAAIDVYGIVDVFAPQGFPT
                     GHPLFSLPNVLLTPHVSAFSEEALAEQRRGGAEAVVQVLSGQWPRHPVNPDVKPWFVL
                     TQEECP"
     gene            complement(1773..2552)
                     /locus_tag="PLQ54_09845"
     CDS             complement(1773..2552)
                     /locus_tag="PLQ54_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOS93603.1"
                     /translation="MTALALEIRDTIVEPRTLAIWYLAQAGFCIKTSMGTRVVIDAYL
                     SDYCGREFGFKRMIPAVIAPDDLRADVLISTHSHGDHLDPDALEAFAAHPATQFIGSA
                     DCESVYVERGVAPDRYTLLRPGDETVIRDVAVRATYADHGELCPEAIGVTIAAEGLRI
                     YNVGDSALRPEEMLRTVEGPVDVMIAPINGAFGNLDAFEACQLGARVRPRLLIAAHFW
                     MFIEHGGDPGRFLEEARNLPAGIEALVMAPGEQRVVRAEES"
     gene            complement(2549..>2840)
                     /locus_tag="PLQ54_09850"
     CDS             complement(2549..>2840)
                     /locus_tag="PLQ54_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOS93604.1"
                     /translation="DMAVIGNDDLSMGMGIAGQLDHPDYIAAVEQVIAACQRHGVLPG
                     IAGGDPDASANWVSRGMRVLWYTSDIYLLWDGAQRQIVALRQRMGSTGKGRV"
BASE COUNT          466 a         1021 c          890 g          463 t
ORIGIN      
        1 cgaccgatcc ggcggcctcg atgacgcgat cgacctgctt ctctttggtg tgccgcagca
       61 cagcctcagc gatggactcc ttctgtgtgt tgaccgccgc gtccactccg agttccagcg
      121 ccttctgaag cttgaagtcc ttgcggccgg tgatcaggac cttcgacgcg ccggagagct
      181 tcgccagctt cgccgccgcg atgccgatgg ggccactccc ctgcacgagc accgtgtcgc
      241 cgatgctgac ccccgctttc accaccgaga agagggccgt ggccgcgggc tcgaccatgg
      301 cgccgttgtc gaagctcacg ctgtctggta acgggaacac gtgccgttcg ggcatcacca
      361 cgtactccgc atacgcacca ggccatgcgt tgattgtgcc gacggcccga gccttcgcgc
      421 agacgccgta gttgcccacc aggcactcgt tgcagtggcc gcacgcgact ccggtgtcac
      481 ccacgacgcg gtcaccgggc gcgaaccgtt ccacggccga acccacatcg gccacgacac
      541 ccgaccattc atggcccggt gtcatgggga acgtgatccc gccgttcttc acgaaccgga
      601 actcgccggt gtagatcgcg tagtcggtcc cgcacacccc ggaccgcacg accctgcaca
      661 gcacgtcgtg gtcaccgagc tcgggaatgg gcacctcgac cacgcgcatg tccatggggc
      721 cgaagatccg ctcggcctgc atggtcttca tgggcattcc tcctgagtca gcacaaacca
      781 cggtttcacg tccgggttca ccggatgcct cggccactgg ccagatagca cctgcaccac
      841 ggcttcggcc ccccctctcc gctgctccgc cagtgcctcc tcggagaacg ccgacacgtg
      901 cggcgtgagc aacacgttcg ggaggctgaa cagtggatgg ccggttggga agccctgcgg
      961 cgcgaagaca tccacgatcc cgtacacatc gatggccgcg tagcggatga tgccctcgcg
     1021 cagcgccgtc acgaggtcat cctcgtccac cagctccccg cggcccgtgt tgatgagcac
     1081 ggccgtgggc ttcatctgcc ggaactgctc cagtgtcagc atgtgccgcg tcgccggtgt
     1141 gagcgggcag agcagagaca ggtagtcggc ctcggccaag gccgtcggca agtcgacgac
     1201 cgataccccc gtctccgccg cggcttccgc ggtgagtgcc ggatcgcagg ccagcacgcg
     1261 catcccgaat gccctggccc gaaccgctac ggcccgccca atgcgtccga agccgatcag
     1321 gctcagcgtg cacgtggcta gccggcgcat atggcgcaca tcgcggggca ccttgccctg
     1381 ccgcgctccg gcctcgaaga gcttgagctg ccgggccgca gccagcagca gcgccatggt
     1441 gtggtccgcg acctcgttgg tacagaagtc cggcaggttc gtcacgaaga tgccgcgccg
     1501 cgtcgccgcc tcgacgtcga tcttgtccac tccggtcccc aggcgcgaga tgatccggca
     1561 ccgcgtgagc tgctcgatca cgctcccacg cacatgggcc gagacaacca tgagcgcatc
     1621 gcagtcacga gcggctgacc gcacttcgtc gtcgctggcc ccctccgcct cgacgatgca
     1681 cgggtccacc gcggcgagga tgtgccgctc cacctcggtg atgggcgcag tctcggcgtt
     1741 cagtcgcgcg atggtccatc ggccggccat cgtcaggact cctccgctcg gacgacgcgt
     1801 tgctcgcccg gcgccatgac cagggcctcg atgcccgcgg gcaggtttcg ggcctcctcg
     1861 aggaaccggc ccgggtcgcc cccgtgctcg atgaacatcc agaagtgggc ggcgatcagc
     1921 aggcggggcc tcacccgcgc gccgagctgg catgcctcga aggcatcgag attaccgaac
     1981 gccccattga tcggtgcgat catcacatcg acgggcccct cgacggtgcg gagcatctcc
     2041 tccggccgca gagcgctatc gccgacgttg tagatccgca gcccctcggc tgcgatggtc
     2101 accccaatag cctcggggca cagctcgccg tggtcggcgt aggtggcgcg gactgccaca
     2161 tcgcgaatca ccgtctcgtc gccagggcgg agcagcgtgt agcggtccgg agcgacccca
     2221 cgctcgacgt agacgctctc gcagtcggcc gagccgatga actgcgtcgc cgggtgagcg
     2281 gcgaaggcct ccaacgcgtc gggatccagg tgatccccgt gtgagtgcgt cgagatcagc
     2341 acgtccgccc ggagatcatc gggagcgatc accgccggga tcatgcgctt gaaaccgaac
     2401 tcgcggccgc agtagtctga gagataggca tcgataacca cgcgcgtgcc catgctggtc
     2461 ttgatacaga agcccgcctg ggccagatac cagatggcca gcgtgcgcgg ctcgacgatg
     2521 gtgtcgcgga tctccagcgc gagggccgtc atacccgccc cttcccggta gaccccatcc
     2581 tctgcctcag cgccacgatc tgtcgctggg caccatccca gagaaggtag atgtccgagg
     2641 tgtaccacag cactcgcatg ccgcggctca cccagttcgc cgaggcgtcg ggatcgcccc
     2701 cggcgatacc cgggagcaca ccgtgcctct ggcatgccgc gatgacttgc tcgacagcgg
     2761 cgatgtagtc cggatggtcg agctgtccgg cgatgcccat gcccatcgat aggtcgtcgt
     2821 tcccgatcac ggccatgtcg
//
LOCUS       DAOSPL010000498         2834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35396_length_2834_cov_2.952861, whole genome shotgun sequence.
ACCESSION   DAOSPL010000498 DAOSPL010000000
VERSION     DAOSPL010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35396_length_2834_cov_2.952861"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2834
                     /locus_tag="PLQ54_09855"
     CDS             <1..>2834
                     /locus_tag="PLQ54_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93605.1"
                     /translation="ESRVFWVRLAVVLAAVIARPGGARPLTGVLELPASTAVTHGSQL
                     RFMDLQGIGCLCYWTDAADWAEWTIDSPRPGPHAVIVRYACQAGSEGATFELRVGGQA
                     LQGAIERHTGTWYSYEDLVVGWVTLPRSGRVAVSLRPTAKPGPAVMNLECIRLVPADR
                     YASYVREMAARAAAAEAHQRCPIYVVPNFHPASCGWLTDFSTERNHCANSYFRHLDRV
                     RDDPQYRFVLSEVNNVMAMMAFQPDRIPELRERAREGRVEFVNAFFLEPTINLSGGEA
                     LVKMGVEGLRWYREVLGVRPRAVWAIDVTGVHEQMGQIVSGLGLDAMVYVRDNPTQRT
                     AHWLESPDGSRTLALCPGPYADWGPIFNTSAALSDDDVRALAADAEVKTLQTPAGLPV
                     LVLGGAGDYSLPPARSEYPSEFLDQWERVVPGRPLRFATLSDYLDVALPAVRSGLVEL
                     PTSRSGARLSWTSFWIENPKVKGWYRSSEHSLAATEALATVASTTAEYTYPAQSLYCA
                     WLQMLLNMDRNTLWGAAGGMVFDHPRSWDVKDRFDWVDEACTRTQQAAMHALGGRGSR
                     LGLFNPVSWERSEPFVLRLSPQTHVTGVPSQQYADGQTLVAASLPAMGTWTPRITAGA
                     PPEPEPADASEAIITRHYCARVDPRTGALCSLRLAPHGRELLAGPVQIVAETGGDGHD
                     TPPPSGRQRLAESARGAARVTAVRGPVATIVRSEGTLIEGRPVSQTIRFYRDSPRIDF
                     DVEVTDVPSGTVVLAEFPLAGSISQVRRGIPYGFSHAAWQVANPALPGYADGIQAAIR
                     WSDYQLTGGGGVAILDRGLPGRELTGSTPTLFLLSAQDKYLGYRCDWLSGRGTHRAAF
                     ALLAHSDAFGQADVARAAYAYNAPPVCVPGVRELPGSSLVRTSPNLVTEALRREGGEI
                     ELRMVECRGAAGTGQVEIRLPHRRAWLTDLV"
BASE COUNT          440 a          952 c          964 g          478 t
ORIGIN      
        1 gagtcgcggg tattctgggt tcggctggcc gttgttctcg ccgccgtcat cgcgcggccc
       61 ggcggagcgc gcccgctcac cggcgtgctg gagctccctg catcgaccgc agtgacccac
      121 ggcagccagt tgcggttcat ggaccttcag ggcatcggct gcttgtgcta ctggacggac
      181 gccgcggact gggccgaatg gacaatcgac tcccctcggc ccggccccca tgccgtcatc
      241 gtacggtacg cctgtcaggc cggatcggag ggcgcgacgt tcgagctcag ggtcgggggc
      301 caggctctcc agggggccat cgaacgccac acagggacgt ggtactccta cgaggatctc
      361 gtcgttggtt gggtgacgct cccccgatcc ggtcgcgtgg ccgtgtcgct acgcccgacg
      421 gcgaagccgg ggcctgccgt tatgaacctc gagtgcatcc ggctcgtccc ggccgaccgg
      481 tatgcctcgt acgtacgaga gatggccgcg agggccgcgg cggccgaggc gcaccagaga
      541 tgccccatct atgtcgtgcc gaacttccac cccgcgagct gcggctggct tacagacttc
      601 tccacggagc gcaatcactg cgccaactct tactttcgtc acctcgaccg agtgcgcgac
      661 gatccccagt acaggttcgt gctgtctgag gtcaacaatg tcatggccat gatggcgttt
      721 cagccagaca ggattcccga gctcagggaa cgcgcgcggg aggggcgagt cgagttcgtc
      781 aatgccttct tcctggagcc caccatcaac ctgtctggcg gcgaggccct ggtgaagatg
      841 ggggtggagg ggctacggtg gtatcgcgag gtgcttggcg tcaggccacg ggccgtgtgg
      901 gcgatcgacg tgacgggggt ccacgaacag atgggccaaa tcgtctcggg cctcggactg
      961 gatgccatgg tctacgttcg ggacaacccg acacaacgga cggcacactg gctcgagtcg
     1021 ccggacgggt cccggaccct ggcgctctgc cccgggccgt acgctgactg gggcccgatc
     1081 ttcaacacga gcgccgccct gtccgacgat gacgtgcgcg ctctggcggc agacgccgag
     1141 gtgaagaccc tacagacgcc ggcaggcctg cctgtgctcg tcctgggtgg tgccggcgac
     1201 tacagtctgc cgccagcccg gtccgagtac ccctccgagt tcctggacca gtgggagcga
     1261 gtggtgcccg gtaggccact tcgattcgcg acgctgtcgg actacctcga tgtcgcactg
     1321 ccagcggtgc ggagtggtct ggtcgagcta cccacctcgc gaagcggcgc ccgtttgtcc
     1381 tggacgtcat tctggatcga gaaccccaag gtgaagggct ggtaccgctc gtcggagcac
     1441 agcctcgctg ccaccgaggc gctggcaacg gtggccagca cgaccgctga gtacacctac
     1501 cccgctcagt cgctctactg cgcctggttg cagatgctcc tcaacatgga ccggaacacg
     1561 ctgtggggcg ccgcgggcgg gatggttttc gaccacccgc gctcgtggga tgtgaaggat
     1621 cgcttcgatt gggttgacga agcgtgcact cggacccagc aggcagccat gcacgccctc
     1681 ggcgggcgcg ggagccgact cgggctgttc aacccggtga gttgggagag aagcgagccg
     1741 ttcgtcctcc gactttcgcc ccaaacacac gtgacggggg tgccatcgca gcagtatgcc
     1801 gacggacaga cgctggtcgc cgcctctttg cccgccatgg gcacgtggac accacggatc
     1861 acagcgggcg cgccgccaga acctgagcct gcggatgcga gtgaagccat cataacgcgt
     1921 cactactgcg ccagggtcga cccgcggact ggcgcgctgt gcagtctcag gcttgcaccg
     1981 cacggccgag agcttcttgc aggacccgtg cagatcgtcg ccgagaccgg cggcgacggc
     2041 cacgacacgc ctccaccgtc cggacggcag cggctggccg agtccgctcg gggggccgcg
     2101 cgcgtcaccg cggttagagg acccgtggca actatcgtcc ggtctgaggg caccctgatc
     2161 gagggcaggc ccgtgagcca gacgatccgg ttctaccgcg attcccctcg catcgacttc
     2221 gatgtcgagg tcacggatgt ccccagcggc accgtggtcc tggcggagtt cccactggcc
     2281 ggcagcatct cacaggttcg gcgcggcatc ccctacgggt tctcgcacgc cgcgtggcag
     2341 gtggcgaacc cggctctacc tggctacgcc gacggcattc aggcggccat ccgctggtcg
     2401 gactaccagc tcactggagg aggcggcgtc gcgatcctcg accgcggcct gcctggtagg
     2461 gagctcacgg gcagcacacc gacgctgttc cttctgagcg cgcaagacaa gtatctcggc
     2521 taccggtgcg actggcttag cggccgaggc acgcaccgag cggctttcgc cctgttggca
     2581 cactccgatg ccttcggcca ggcagacgta gccagggcgg cctacgcgta caacgcgccg
     2641 ccggtctgtg tcccgggcgt ccgggagctt cccggttcct cgctcgttcg gacttcgccg
     2701 aacctggtta ccgaggcgtt gaggcgcgag ggcggtgaga tcgagctgcg gatggtcgag
     2761 tgccgcggcg ctgcgggcac tgggcaggtc gagatcaggc tcccccatcg gcgggcctgg
     2821 ctcaccgacc tcgt
//
LOCUS       DAOSPL010000499         2834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35397_length_2834_cov_2.857503, whole genome shotgun sequence.
ACCESSION   DAOSPL010000499 DAOSPL010000000
VERSION     DAOSPL010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35397_length_2834_cov_2.857503"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1977)
                     /locus_tag="PLQ54_09860"
     CDS             complement(<1..1977)
                     /locus_tag="PLQ54_09860"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOS93606.1"
                     /translation="MPSTPPMSARLNSVAEAIRHRRVMPHTSPKEANITHAFFREYAD
                     LPLAERQARSVVYALEREPIHYLEDEAIAGQVFQGCPGSGDPLLNGTADPALLEASGG
                     QWPEFCVAGAGARWVAERLPEHALYGRWFSDGAAPGHIGWDWRLVLELGFEGLLERYR
                     AAMASPADDRAAEFYRCACIALEGAIAFADRLASGASPALRSVCQRVPRRPSTTFAEA
                     VQAFWTQYLAVMFENPYGGNGPGLVDRFLWPYLERDLAAGTTTLDEARDLVRELFLKL
                     DERIHPHDGWVEAICVGGHTADGRGAESPLSRLIVETIMELNQTHPSVYIRLRDDAPP
                     DFRRLAADYIVGGGNRAQVYGDDRVIEALVASGHRREDACEWMAGGCMEVSSQARNCD
                     FNFTYAHNAAWTLEAVLYGGTVPHHGPARVSPIEADLTAYSDFDGLFAAFESELRREL
                     DLVFRRLDIYFEAYARYRPAFLISTMVYDCLERGRGLNDGGARYADFCGSVVGIPDVA
                     DSLLALKRALFDEGFCQASEMLEALRADFTGHEALRARLSALPKYGQDDPDADAMCNR
                     VLGVYCDACEAHRTPHGGKVRPLVLGFVWVAEFGAITGALPDGRKAGQPLAHGLAPQG
                     GAAIHGLSAALNSATSLDLYRVPGGASMVFDLDTS"
     gene            2118..>2834
                     /locus_tag="PLQ54_09865"
     CDS             2118..>2834
                     /locus_tag="PLQ54_09865"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOS93607.1"
                     /translation="MPSRSEDAAILTIHAGPHARTHTVIRVPRPTALSGPELSFEPEG
                     GGEPWAAQIDRADPETLWLLVPGLAAGAEVRLRPVPHAAPRPGVALTEENDTIEVWVG
                     GELFTAYHYGAALARPYLYPVVGPKGARVTRECRWEDGPGFDHKHHKSVWIAHGLVNG
                     TDNWSEEPGHGSTRHTGFDEVTDGPVMGRIVETTEWLSASGRKVLDETRVLRFYAQPA
                     ECRCLDVEITFRTGHDAVLFG"
BASE COUNT          477 a          976 c          976 g          405 t
ORIGIN      
        1 cgacgtgtcg aggtcgaata ccatgctggc accaccgggc acccggtaca gatccagcga
       61 cgtcgccgag ttgagcgccg ccgagaggcc gtggatggcc gcaccgccct gaggcgcgag
      121 gccgtgcgcc agcggctgac ccgccttccg gccgtcgggc agcgcaccgg tgatcgcccc
      181 gaactcagcc acccacacga aacccagcac cagggggcgg accttcccac catgaggggt
      241 gcggtgcgcc tcgcaggcat cacagtagac gcccagcacc cggttacaca tagcatcggc
      301 atcggggtcg tcctggccgt acttgggcaa ggccgagagc cgggctcgca gggcctcatg
      361 cccggtaaag tccgctcgga gggcctcaag catctctgag gcctggcaga aaccctcgtc
      421 gaacaaggca cgcttgagag ccaagaggct gtcggctacg tccgggatgc cgacaacgct
      481 cccgcagaag tccgcgtagc gtgccccgcc atcattgagc ccccggccgc gctcgagaca
      541 gtcgtagacc atggtcgaga tcaggaaagc ggggcggtac ctggcatagg cctcgaagta
      601 gatgtcgagg cggcgaaaaa ccagatcgag ctctcggcgt agctccgact cgaaggcagc
      661 gaagaggccg tcgaagtcgc tgtaggcggt gaggtcggcc tcgatgggcg agacgcgcgc
      721 cggaccgtgg tggggaaccg taccgccata gagcacggcc tcgagcgtcc acgcggcgtt
      781 atgggcgtag gtgaagttga agtcgcagtt gcgcgcctgg ctggagacct ccatgcaccc
      841 gcctgccatc cactcgcagg catcctcgcg ccgatgcccg gatgcgacca gggcctcgat
      901 gactcgatca tcaccataaa cctgggctcg gttcccgccg ccgacgatgt agtcggccgc
      961 gagccgacgg aagtcgggtg gcgcatcgtc gcgcaaccgg atgtacaccg acggatgcgt
     1021 ctggttcagc tccatgatgg tctcgacgat caggcgagag agcggcgact cggcgccccg
     1081 cccgtcggcg gtgtgccctc ccacgcagat ggcttccacc cacccgtcgt gtgggtggat
     1141 gcgctcatcg agcttcagga acagctcacg cacgaggtca cgcgcctcgt cgagggttgt
     1201 cgtgccggcc gccaggtcgc gctcgagata gggccacagg aaccggtcga ccagccccgg
     1261 cccgttgccg ccgtaggggt tctcgaacat gacggccaga tactgggtcc agaacgcctg
     1321 cacggcctcg gcgaaggtgg tcgacgggcg ccgaggcacc ctctggcaca ccgagcggag
     1381 agcgggagag gccccactgg ccagccggtc ggcgaaggcg atggccccct cgagcgcgat
     1441 gcaggcgcaa cgatagaact cggcagcccg gtcatcggcg ggcgaggcca tggcggctcg
     1501 gtagcgttcg agcaagccct cgaagcccag ttcgagcacc aggcgccagt cccacccgat
     1561 gtggccgggc gcggcaccgt cgctgaacca ccggccgtag agggcgtgct cgggcagtcg
     1621 ctcggccacc caccgagcac ccgcgccggc cacgcagaac tcgggccact gccccccact
     1681 ggcctcaagg agtgccggat cggccgtccc gttgaggagc ggatcgccag accccgggca
     1741 gccctggaac acctgcccgg cgatggcctc gtcttccagg tagtggatgg gctctcgctc
     1801 aagcgcgtag accacacttc gcgcctggcg ctcagccaac ggaagatcgg cgtactctcg
     1861 gaagaatgcg tgcgtgatgt tcgcttcctt ggggctcgtg tgaggcatga cccgccggtg
     1921 gcggatcgcc tcagccacgg agttcaggcg ggcactcatg gggggcgtac tgggcatcgg
     1981 gcagtctccc ggggcggcag tcgacgggca ttcgggggca ttcttcggca tggcccggcc
     2041 atgccctccc gatggaagga ccgcgcgcgg atgcggacga acttccccac tacccaacac
     2101 accgggaggc ctgctccgtg ccatcgcgct ccgaggacgc cgccattctg accatccatg
     2161 ccgggcctca cgctcggacg cacaccgtca tccgcgtccc gcggcctacg gccttgtcgg
     2221 gccccgaact gagcttcgag cccgagggcg gaggcgagcc ctgggcggcg cagatcgacc
     2281 gagcggaccc ggagaccctg tggctgctcg tacccggcct ggcggcgggt gccgaggtcc
     2341 gtctgcggcc cgtgccacac gcggcccccc gacccggcgt cgctctgact gaggagaacg
     2401 acaccattga ggtgtgggtg ggcggggagc tgttcacggc ctaccactac ggcgctgccc
     2461 tggcgcgtcc ctacctctac cccgtggtcg gcccgaaggg cgcccgcgtg acccgtgagt
     2521 gccggtggga ggacgggccc ggtttcgacc acaagcacca caagtccgtc tggatcgccc
     2581 acgggctggt caacggcacc gacaactgga gtgaggagcc cgggcatgga tcgacgcgcc
     2641 acacggggtt cgacgaggtg accgacgggc cggtgatggg gcgcatcgtc gaaaccaccg
     2701 agtggctgag cgcctcgggc cgcaaggtgc tcgacgagac ccgcgtgctg cgtttctatg
     2761 cccagcctgc cgagtgccgg tgtctcgacg tcgagatcac cttccgcacg gggcacgatg
     2821 cggtgctctt tggc
//
LOCUS       DAOSPL010000500         2833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35409_length_2833_cov_2.898128, whole genome shotgun sequence.
ACCESSION   DAOSPL010000500 DAOSPL010000000
VERSION     DAOSPL010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35409_length_2833_cov_2.898128"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1589
                     /locus_tag="PLQ54_09870"
     CDS             <1..1589
                     /locus_tag="PLQ54_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOS93608.1"
                     /translation="PERLTGGGRAVANLGHILAIDVGTGAVRAALFAADGRLLSSVAS
                     VPYEPQWVPERPDFSQVDPAAWLDAIETAIGILRAESSLGSVAAVGVSGLFPALIAFD
                     SAGHPLGPAILYSDRRSQTELEAIRLAGIGEAMEQLTGSALTPGTTSVTSLLWLRNHE
                     PATFARATTVGHVNTLIGHWLTGQWGMDRSNASLTGLFDTRAGHWSATLCSALGIDLG
                     LLPPLIEGSRPVGALLAPAANHLGLPEGIPVAMGGGDTLCAALGCGATAPGDFYISSG
                     TTDTLGLCLDRYAYDPRLYCCGHVVPDRYVSIAPMTYTGGCLRWFVSQFCGEADYAAL
                     ERAASDVPPGSGGLVFLPYLKGERSPVHDPDARGVYFGLTDTTTRDAMGRAVLEGVAY
                     GIRHCLEAMEQAHSVRVAELVVVGKPGRSGLWNQIKANVTGRRVRALGFDERSLLGAA
                     VTGALAAGLLRAETEAGGRLAGLEAFLAPHAAAERVYDPGDETRAIYDRLYGVFTDLY
                     TVARGAGLFERLYDTGTHRP"
     gene            1854..>2833
                     /locus_tag="PLQ54_09875"
     CDS             1854..>2833
                     /locus_tag="PLQ54_09875"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS93609.1"
                     /translation="MMVLPSIDERRYAPCDVLVAGGGMAGISAAVAAARMGARTVLVE
                     QAGWLGGLGVTGATGLHSFFNIAGAHRGAPLMRVVGGIGQELVDAVQRLAGGVGHVRM
                     ERGDDFVTMLTPVEPETFKLAAARLCTEAGVRLLLHTVVDEIHSTRGAVEGVVVWNKA
                     GRSLLRARVYIDATGDGDLAAHAGVPFERFRPGDPGAYSAGFTFRLCNIDLAAMEADL
                     EGRGVITQLAHATKPGMAAPDLVRIGIDMQRLGAGLSDPAPSYFLASSLRPRELTCCN
                     CINYGPNDGLDAGALTEAEIDLRWRMFEVASFFRRHVAGCSEAYPAGAAPS"
BASE COUNT          401 a          986 c          981 g          465 t
ORIGIN      
        1 gtcccgaaag gctgaccggc ggtggtcgag cagtggcgaa cctggggcac atcctggcaa
       61 tcgacgtcgg cacgggggcc gtgcgagccg cgctgttcgc tgccgatggt cgcctgctct
      121 cgagtgtcgc ttcggtgccc tacgagccgc agtgggtgcc cgagcgtccg gacttctcgc
      181 aggtcgatcc cgcggcgtgg ctggacgcga tcgagacagc catcggcatt ctccgtgccg
      241 agagtagcct ggggtcggtg gccgccgtcg gcgtgagtgg gctgttccct gcgctcatcg
      301 cgttcgactc ggccggtcat ccgctgggtc cagccattct ctactccgat cgccgcagcc
      361 agaccgagct cgaggccatt cggctggcgg ggattggcga ggccatggag cagctgactg
      421 gcagtgccct gacgccgggc accacctcgg tcacgagcct gctttggctg cgtaaccacg
      481 agccggcaac attcgcccga gccaccacgg tcggccacgt caacacgctg atcggccact
      541 ggctcaccgg acagtggggt atggatcgaa gcaatgcctc gctgacgggg ctctttgaca
      601 cgcgcgcggg ccattggtct gccacactgt gctcggcgct ggggattgat ctcggcctgc
      661 ttcctccact gatcgagggc tcccggcctg ttggcgcgct gctagcgcca gccgcgaacc
      721 acctcggcct gccggagggc atccccgtcg ccatgggcgg aggtgacacg ttgtgcgccg
      781 ccctggggtg cggcgccacg gcgccgggcg acttctacat atcgagcggc accaccgaca
      841 ctctcgggct gtgcctcgat cggtacgcct atgatcctcg gctctactgc tgcggccatg
      901 tcgtcccgga ccggtatgtc tccatcgcgc ccatgacgta cacggggggc tgcctgagat
      961 ggttcgtgtc gcagttctgc ggcgaggcag actacgccgc gctcgaacgc gccgcctcgg
     1021 acgtaccgcc gggctccggc ggcctggtgt tcctgccgta cctgaagggc gagcgctcgc
     1081 cggtccacga tcccgacgca cgcggcgtct acttcgggct gaccgatacc accacgcgtg
     1141 acgccatggg gcgagccgtg ctcgaaggcg tggcttacgg gatccgtcac tgcctcgagg
     1201 ccatggagca ggctcacagc gtccgggtgg cggagctggt cgtggtcggc aagccgggcc
     1261 gatcgggcct atggaaccag atcaaggcca acgtgaccgg gcgccgagtg cgcgcactgg
     1321 gcttcgacga acggtcgctg ctcggcgccg ccgtcaccgg cgcactggcg gccgggttgc
     1381 tccgcgccga gaccgaggcc gggggccgtc tggccggcct ggaagccttt ctcgcgcccc
     1441 atgcagccgc cgagcgcgtc tacgaccccg gcgacgagac ccgggccatc tacgatcggc
     1501 tctacggcgt gttcacggac ctgtacacgg tcgctcgcgg cgcggggctg ttcgagcggc
     1561 tatatgacac cggcacacat cgcccctgag acgtagtcga gagcgtgaca acgcgcggtg
     1621 cccacaggcc cggggttgga cacggccgag accgcaaatg gccgaagccc ttcaccctgc
     1681 gacgatccgg accggtctcc ggtcttggtg gctcgacgcg cgcccaatgc agcgaagtcg
     1741 acgaaggccc accgacccgt gcgcccgccc gctccttgcc tcgccttggg ggctggctta
     1801 tcatcgtggc tccccggcca cggccagaca agtcggcaga agacacggag gcaatgatgg
     1861 ttctaccaag tatcgacgag cggcggtacg ccccctgcga cgtgctggtg gcgggtggcg
     1921 gcatggccgg gatctcggcc gcggtggccg ccgcgcggat gggggcccgc accgtgctcg
     1981 tggagcaggc cggatggctg ggcggactcg gggtcactgg ggccacgggc ctccatagct
     2041 tcttcaacat tgccggtgcg caccgcggcg cgccgctcat gcgcgtcgtg ggcggcatcg
     2101 ggcaggagct cgtcgacgcg gttcagcggc tcgccggcgg agtcgggcat gtgcgcatgg
     2161 agcgcgggga cgacttcgtg accatgctga cgccggtcga gcccgagacc ttcaagctgg
     2221 ctgcggcccg cctctgcacc gaggcaggag tccggctcct gctgcatacc gtggtcgacg
     2281 agatccactc gacgcgaggc gccgtcgagg gcgtggtcgt ctggaacaag gccggacgca
     2341 gtctgctgcg cgcccgagtc tacatcgatg ccaccggcga tggcgacctc gccgcacatg
     2401 ccggcgtgcc tttcgagcga ttccggcccg gtgaccccgg cgcctattcg gctgggttta
     2461 cgttccgtct ctgcaacatc gatctggccg ccatggaggc cgacctcgag ggccgcggcg
     2521 tcatcaccca gctcgctcac gccaccaagc cgggcatggc tgcgcccgat ctggtccgga
     2581 tcggcatcga catgcagcgc ctgggagcgg ggctctcaga cccggccccg tcctacttcc
     2641 tggcatcctc cctgcggccg cgcgagctga cctgctgcaa ctgcatcaac tacggtccca
     2701 acgatggcct cgatgccggc gcactgaccg aagccgagat cgatctgcgc tggcgcatgt
     2761 tcgaggtggc ctcgttcttc cgtaggcacg tggcagggtg ctcggaagcc tacccggcgg
     2821 gagccgcccc ctc
//
LOCUS       DAOSPL010000501         2828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35481_length_2828_cov_2.943743, whole genome shotgun sequence.
ACCESSION   DAOSPL010000501 DAOSPL010000000
VERSION     DAOSPL010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35481_length_2828_cov_2.943743"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            441..1580
                     /locus_tag="PLQ54_09880"
     CDS             441..1580
                     /locus_tag="PLQ54_09880"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOS93610.1"
                     /translation="MASSEGTRATIEHLSEAIGPRPCGSEGEHRAAEWVASRLRDLAF
                     EVTQETFRCAPGFALTFLLLTLTSFAALGLMGRCPACALAMALAALIGFVAEGLGRPV
                     VSRLLPQTESRNTVGRRAATARAARVVVVTAHIDSAYAGLVFRPRVVRWFRAIFVGLL
                     GAMIYTLVLCALQLRCTVPDWVTLPAAAHLYWVALCLTHQVIWAKPVPGAGDNASGVA
                     ALLAVAEELPPLATTEVWLVATGAEEAGLYGMSHLLSSHAFARDRTMFVNIDNVGAGP
                     LRLISHEGILVPMAADPGILEAAKKAMAALGLECRVEAYRTLPVESAVALASGYRALS
                     IMGSLQHWHQECDVAARVDASVAAQAAGVVTEMLVRWDSATPAVD"
     gene            2259..>2828
                     /locus_tag="PLQ54_09885"
     CDS             2259..>2828
                     /locus_tag="PLQ54_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOS93611.1"
                     /translation="MTPAARTEVKVGAMVIVAVALLFGALRAIKNHGLTAGHMPVSII
                     FGELTESLPPGSPVKLAGVTVGRVDTMELVLPQEVTAVPAGFEDAGIVVRLRCRIQRA
                     ARLRQGYQATVRSGFPLGQTFVDIMPTRQSGPLLDPGGTLVGYVAPGMMDIAPMTAEA
                     LQEIRRAAEGIATATRSIESAATDVGTGAR"
BASE COUNT          412 a          967 c          976 g          473 t
ORIGIN      
        1 gatcctcatc gaccagccgg cccactcggt tgtctcccgc gtggggtact cccatggcca
       61 accccccctc cacctcgttg acgcccgcgc caacagtgcc ggctcgccaa ctcactcaca
      121 ccccaccgtg ctgagtcgcg acgtccacgc ggtggctaga caggcaacta ctgcactcca
      181 gcaccgcgtg cggtctgact catgttccgc tcttcgatgc ggtggaacgg cctgactacg
      241 gggctgtcgt cctctaggtt cgaccacgga tccgctgggc tgttgcgtag actgactcac
      301 tatccagtgt agtcctgcag agaccgggcg tcaacgagcg gcgagcgacg agcgccagga
      361 gcctgacgtc cggagcccga agtcagaagc cagtagtccg aagcatggtt cggcattccc
      421 gtgagctgga aagggaagcc atggcgagtt ccgagggcac tcgagccacc atcgagcact
      481 tgtcggaggc gattgggccg cggccgtgcg ggtccgaagg cgagcaccgg gctgccgagt
      541 gggtggccag ccgactccgc gacctggcct tcgaggtcac ccaggagaca ttccgctgcg
      601 cccctggctt cgccctcacc ttcctgctcc tgaccctcac cagcttcgca gccttgggtc
      661 tgatggggcg gtgcccggcg tgcgcgctgg ccatggccct cgcggccctg atcgggttcg
      721 tggccgaggg gctaggtcgg cccgtcgtca gccgcctgct gccgcaaaca gagagccgga
      781 acacggtcgg ccgccgagcc gcaaccgccc gggcggcgcg tgtggtggtt gtcacggcac
      841 acatcgactc ggcctatgcg gggctagtct ttcgcccgcg agtggtgcgg tggttccgtg
      901 ccatctttgt cgggctcctg ggcgccatga tctacacgct ggtgctctgc gcgctgcagc
      961 tccggtgcac cgtgcccgat tgggtcaccc tccccgcggc cgcgcatctg tactgggtcg
     1021 cgctctgcct gactcaccag gtgatctggg cgaagcccgt tccaggcgca ggtgacaacg
     1081 cctcgggcgt cgcggcactg cttgcggtgg ccgaggaact gccccctctg gcgacgaccg
     1141 aggtgtggct cgtggccaca ggagccgagg aggccgggct ctacgggatg agtcacctgc
     1201 tgtcctcgca tgcgttcgcc cgcgaccgta cgatgttcgt caacatcgac aacgtgggcg
     1261 ccgggccgct gaggctcatc tcgcacgagg gcatcctggt ccccatggct gccgaccctg
     1321 ggatcctgga agccgcgaag aaggcgatgg cggcgctcgg gttggagtgc cgggttgagg
     1381 cctaccggac gctcccggtc gagagcgccg tcgctctggc cagcggctac cgggctctga
     1441 gcatcatggg cagcctccag cactggcacc aggagtgcga cgtggcagcc cgggtcgatg
     1501 cgtctgtggc ggctcaggcg gcgggtgtgg tcacagagat gctggtgcgg tgggactcgg
     1561 cgacgcccgc cgtggactga ggaggggcac cgcagccctg cccttgccgt cgaagcgcgg
     1621 ctcccggtca gcctgcgccg gtcgggttct ggaaccgccg cccgtgggcg gcggacgtcg
     1681 cccgggcccg actcgtcacg gccaagggag gcgcgcggtg cccgaggtcc gcgagttcgc
     1741 aagtgacgac gccggttcct cgcgtctctt cgggtgccca gggcgggact cgaacccgca
     1801 cggccgcatg ggccaaagga ttttacacat atcccccggt ttcccggggg ggtggactat
     1861 ctcttcatct ccgcgaacca ggcggcgaag atgccgggcg ctcgagtggc ggtattgccg
     1921 tcgcggccaa cgcagccgcc ccaggctcag ccatagtctc tgcaccttcc cgggggtttc
     1981 cccccgggct tggctcagga tcagcatatc ggcacccggc gtcgcaccag ccgacttagc
     2041 cttccctgag ttcacccggt ttgttacccc actgtcacca gcggggagac cgccatccag
     2101 tcccttgtgt ctgccggttc caccacctgg gcacacccgg tccagtatag ccttggggca
     2161 cgacccgggg aacaggccgg cgccgaggag ggacgccccc gtcgggcatt gaactgtttc
     2221 acgacactcc cgatgccgcc caacccgagg ggacgccgat gacgccggcc gcgcgaacag
     2281 aagtgaaggt cggcgccatg gttattgtgg ccgtggcgct gctgtttggc gcgttgcggg
     2341 ccatcaagaa ccacggcctg accgcgggcc acatgccggt gagtatcatc ttcggcgagc
     2401 tgacggagag cctgccgccg ggaagcccgg tgaagctcgc cggtgtcacc gtcgggcggg
     2461 tcgacacgat ggagttggtc cttccccagg aagtgaccgc ggtgccggcg ggtttcgagg
     2521 acgctgggat agtggtgcgg ctgcgctgcc ggatccagcg tgccgcgcgt ctgcggcagg
     2581 gctaccaggc caccgtgcgg tcaggctttc cactcgggca gacgttcgtc gacatcatgc
     2641 ccacaaggca gtcggggccc ctcctggatc ccggcggcac actggttggc tacgtggcgc
     2701 cgggcatgat ggatattgcc ccgatgacgg cggaggccct gcaggagatc cggcgcgctg
     2761 ccgaggggat cgccacggcc acccgctcga tcgagtccgc ggccacagac gtgggtaccg
     2821 gcgcgcgg
//
LOCUS       DAOSPL010000502         2825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35520_length_2825_cov_3.372563, whole genome shotgun sequence.
ACCESSION   DAOSPL010000502 DAOSPL010000000
VERSION     DAOSPL010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35520_length_2825_cov_3.372563"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2688)
                     /locus_tag="PLQ54_09890"
     CDS             complement(<1..2688)
                     /locus_tag="PLQ54_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HOS93612.1"
                     /translation="MSSTRIDGLVSGIDTASLIKALLQTEQDRVDKLYDQRDELLFEQ
                     EAFRDVNSKLYELQQVALALRLESTFKTKIVTSSDEAVLRATATAAAARGTHIVKISQ
                     VAEAARAESEVVTTYSRTDVTGTAGLAQAGGTATGTTEGTHSLTFTSGRGGRIVGRVT
                     DLTGDETMADLGATVLNGLRFSLQGVTGRLTSVKSTTTVNELINLINTGVPLVRAELV
                     SGRLVLTAAQGDCDFSVSDTNYTSGILAKVLGFSAETASVAEVVGGVQGLTASTTLGE
                     IGVSTFDQFGIIIGGQRMSLSTGGPFNASTTIATLMAAIEADAPGVSAELTADGRLAL
                     RSDLVGVGFTLADDNYSDDIASKVFGLSGRQSTQARGAGNALVTAAASGARILGGVAG
                     VTASTTLRSLGVGTANGLTITVEGGSSFTVSGLNTSSSLQDLVDAINAGQSNVTAQIV
                     TGRLLLTANDGGRNFTVSDANYTNGVATCALGITAAQSTADSPRGGATAPIVSAAAAG
                     KRGKIVGAVGGLTGTTTLGALGITTDARTNTLGLWVDDVSYGIAGLTTASTVNDLIAA
                     IEDSGAPVNAYLDDGHLVIEAEDNNGNVTLYDTGYYDDGVVGNVLGFTGIRSSVATIT
                     SAVTGLTRSDILGAGFGGGITDTDWFTITLADGTSTTLQFTSTPPTVGQFIDAVNAQV
                     TGVRAALTANGAVELAATSVGMNFSVADRDYVGTIAGELFGLTRGQSTILPSSGNARV
                     TSASTGSQIVGTEIYLLSADFADGDPTAGGAITFSSGDETQYSVSSQQLYKAAAAAGA
                     DTDRAIFGDASLTDYAIEFNARVATTINHAVTAEVRYDSATGNSYRVDFADASHALGE
                     GVHIYYNTGGGDTLLGTLAIPTGFDISTQHTYRVEVEGKN"
BASE COUNT          429 a          899 c          986 g          511 t
ORIGIN      
        1 gttcttcccc tcgacctcga cgcggtacgt gtgctgggtg gagatgtcga acccagtggg
       61 gatcgcgagt gtgcccagca gtgtgtcgcc gccgccggtg ttgtagtaga tgtgcacacc
      121 ttcaccgagg gcgtggctcg cgtccgcaaa gtccacccgg tagctgttcc cggtggcact
      181 gtcgtagcgc acctcggccg tcaccgcgtg gttgatggtg gtggcgaccc gggcgttgaa
      241 ctcgatggcg tagtcggtga gcgaggcgtc gccgaagatg gcccggtccg tatcggcgcc
      301 ggcggcagcg gcggccttgt agagttgctg agagctcacc gagtactggg tctcatcgcc
      361 ggagctgaag gtgatggccc cgccggcggt cgggtcgccg tcggcgaagt cggcgctgag
      421 aaggtagatc tcggtgccca cgatctggct gccggtgctg gccgaggtga cccgagcatt
      481 gcctgaggac ggcaagatcg tcgactgacc ccgggtgagt ccgaacagct cgccggcgat
      541 ggtgccgaca tagtcacgat cggccaccga gaagttcatt cccaccgagg ttgccgccag
      601 ctccacggcc ccgttggcgg tcagcgcggc ccgtacgccc gtgacctgtg cgttcaccgc
      661 gtcgatgaac tgtccgaccg tcggcggggt gctggtgaac tggagcgtgg tgcttgtccc
      721 gtccgccagc gtaatggtga accagtcggt gtcggtgatg ccgcctccga acccggcccc
      781 cagtatgtcc gaccgcgtca gcccggtcac ggcgctggtg atggtggcca ccgaggaccg
      841 gatgccggtg aaccccagga cattgccgac gaccccgtca tcgtagtagc cggtgtcgta
      901 gagggtcacg ttgccgttgt tgtcctcggc ctcgatgacc agatgcccat catcgaggta
      961 ggcgttgacg ggagcgcccg agtcctcgat ggcggcgatg aggtcgttga cggtgctggc
     1021 cgttgtcaag ccggcgatgc cgtaggagac gtcatcgacc cagagcccga gtgtgttggt
     1081 tcgcgcgtcc gtggtgatgc ccagtgcacc cagggtggtc gtgccggtca gaccacccac
     1141 cgcccccacg atcttgccgc gcttgccggc cgccgccgcg gacacaatgg gcgccgtggc
     1201 tccgccgcga gggctgtccg cggtggactg cgcggcggtg atgcccagcg cgcaggtcgc
     1261 gacaccgttg gtgtagttgg cgtcggacac cgtgaagttg cggccgccat cgttggcggt
     1321 cagcaggagc cgtcccgtga cgatttgggc ggtcacgttg gactggccgg cgttgatcgc
     1381 gtcgaccagg tcctgcagcg aactcgacgt gttcagtccg gacaccgtga aggaactccc
     1441 gccctcgacc gtgatggtca agccgttggc ggtgccgaca cccagagacc ggagtgtggt
     1501 ggacgccgtc acgccggcga cccctccgag gatccgtgca ccggatgcgg ccgcggtcac
     1561 cagggcgttc cctgcccccc gggcttgcgt actctggcgt cccgatagtc cgaacacctt
     1621 ggacgcgatg tcgtccgagt agttgtcgtc ggccagggtg aagcccaccc cgaccagatc
     1681 ggaccgcaaa gccagacgcc cgtcggcagt gagctcggcc gagacgccag gcgcgtcggc
     1741 ctcgatggcg gccatcagcg tggcgatggt ggtgctggcg ttgaaggggc ccccggtgga
     1801 gagcgacatc cgctgcccgc cgatgatgat gccgaactgg tcgaaggtgc ttaccccaat
     1861 ctcgcccagg gtcgtgctag ctgtaaggcc ctggactcca ccgaccacct cggccaccga
     1921 ggccgtctcg gccgagaagc cgaggacctt ggccagaatc ccgctcgtgt agttggtgtc
     1981 ggatacgctg aagtcgcagt cgccttgggc ggccgtgaga accaatcgcc cgctgaccaa
     2041 ctcggcccga accaatggaa cgcccgtgtt gatcaggttg atgagctcgt tgactgtggt
     2101 ggtggacttc accgaagtga gccgccccgt aacgccctgt aggctgaagc ggagcccgtt
     2161 gagtaccgtg gcacccaggt cggccatggt ctcgtcgccg gtcaagtcgg tgacgcgtcc
     2221 cacgatgcgc ccaccccgcc cgctggtgaa cgtgagcgag tgagtcccct cggtggtgcc
     2281 cgtggcggtt ccccccgcct gggcgagtcc ggccgtcccc gttacatcgg tgcggctgta
     2341 ggtggtgacg acctcgctct cggcccgagc ggcttcggcc acctgactga tctttacgat
     2401 atgggttccc cgggcggcag ccgccgttgc ggtcgcgcgg agaacggcct cgtcggatga
     2461 ggtgacgatc ttcgttttga acgtcgactc caggcgcagg gccagggcga cttgctgaag
     2521 ctcgtataac ttgctgttga cgtcgcggaa tgcttcttgc tcgaacaaca gctcatcgcg
     2581 ctggtcgtag agcttgtcga cgcggtcctg ctcggtctgc agcagcgcct tgatcaggct
     2641 tgcggtgtcg atgcccgaga ccaggccgtc gatgcgggtg gaggacatag caggaccaac
     2701 tccgatggcg gcattcggcc atgcgccagt aaggcttatc ggcaaccagg gtccggagct
     2761 tgactggaga ggatccggca ccgggcctgg gcgatagggt ctgacgcgct caggcccggg
     2821 ccagc
//
LOCUS       DAOSPL010000503         2823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35563_length_2823_cov_2.693642, whole genome shotgun sequence.
ACCESSION   DAOSPL010000503 DAOSPL010000000
VERSION     DAOSPL010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35563_length_2823_cov_2.693642"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /gene="gatB"
                     /locus_tag="PLQ54_09895"
     CDS             <1..1246
                     /gene="gatB"
                     /locus_tag="PLQ54_09895"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOS93613.1"
                     /translation="SHSIFARKNYWYPDLPKNYQISQYELPLCRDGYLDIDVNGQKRR
                     IRIRRVHLEEDTGKLYHTAGNESRIDFNRCGVPLMEIVSEPDMRSADEAREYLVALRQ
                     VLRYIGVCTGNMEEGSFRCEPNVNVRDTESGAKTGISELKNLNSFRAVHQSVDFEIAR
                     HTRLLQRGETVPRQTRRWHPSDESRGGYTDVMRSKEEAHDYRYFPEPDLVPMRIGDDW
                     LSEIRAGMPELPQQKRERFVSQYALTPYDAGVLTQSLEFAQYFEAAVAACRAGQVAEA
                     AKRVSKWLQGEMSRLLNETGQDIADVPARPEHVAELLELVEEGKLTGTIAKEVFEEMF
                     RTGKPAPQIVSERGIEVVGDASELERIVDQVLADHAEVVAKVKSGKTQSKGFLVGQVM
                     KATRGQAVPQIVNDIIDRRLGV"
     gene            1420..2709
                     /locus_tag="PLQ54_09900"
     CDS             1420..2709
                     /locus_tag="PLQ54_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOS93614.1"
                     /translation="MTTHRILAGAAAVAVLALLARLSLLSYGLYVLILALLASELLAR
                     FSLDRIECLRDVRVDRAKIGDQVVVSTIIKNNKPIPVPWMLAEDALPRRLPVSGETTR
                     LLVLGPYGDARLHYSITMAHRGYHQIGPVVLESGDLFGFVRKFRAARKAHYITVQPDT
                     VPILRYDVASRRPIGDIKVRRLLHDDPSRLAGVREYQRGDPLNRIHWKATARTGALHS
                     KVYEHTTLAGAMIAMDFHGPSFEGDEDRQFIRSEMAVKVAASLAAYLVSLRQQVGFIS
                     NGLDAAERAKREEGLLDARSRREARNFAASRKGADRLRPVEVAARKGHDQAMAVLDAL
                     ARLELSDGCPFEQMLLDEHTRLPRDLTLLVVVPRMNPLLYQVLARVRFSGFSIMVFLV
                     DNAEQAHGARGALAAQGIGFMHVARLDDLQHVAHSGI"
BASE COUNT          488 a          990 c          885 g          460 t
ORIGIN      
        1 atcccacagc atcttcgccc ggaagaacta ctggtacccc gatctgccca agaactacca
       61 gatttcccag tacgagcttc cgctgtgccg cgatggctat ctcgatatcg acgtgaacgg
      121 ccagaagcgc cgcatccgca tccgccgcgt gcacctcgag gaggacaccg gcaagctcta
      181 ccacacggca ggcaacgaga gccgcatcga cttcaaccgg tgcggtgtgc cactcatgga
      241 gatcgtctcg gagccggaca tgcgctcggc cgatgaagct cgcgagtacc tcgttgccct
      301 gcgtcaggtt ctccgctaca tcggcgtctg caccggcaac atggaggaag gctccttccg
      361 ctgcgagccc aacgtcaacg tgcgtgacac cgagagcggc gccaagacgg gtatcagtga
      421 gctcaagaac ctgaactcct tccgtgcggt gcatcagagc gtcgacttcg agatcgctcg
      481 gcacacccga ctgctccagc gcggcgagac cgtgccgcgc cagacgcgcc gttggcatcc
      541 gtcggacgag tcccgcggcg gctacaccga tgtgatgcgc tccaaggagg aggcgcacga
      601 ctatcggtac ttccccgagc ccgacctcgt gccgatgcgc atcggcgacg actggttgag
      661 cgagatccgc gcgggcatgc ccgaactgcc ccagcagaag cgcgaacggt tcgtcagcca
      721 gtatgcgctg acgccctacg acgcgggggt gctcacgcaa tccctcgagt tcgcccagta
      781 cttcgaggcc gccgtggccg cctgccgagc cggccaggtc gccgaggctg ccaagcgggt
      841 cagcaagtgg ctccagggcg agatgtcccg actgctcaac gagacgggcc aggatatcgc
      901 cgatgtgccg gcgcgtcccg agcacgtggc cgagctgctc gagctcgtcg aggaaggcaa
      961 gctcaccggc accatcgcca aagaggtctt cgaggagatg ttccgcaccg gcaagccggc
     1021 gccgcagatc gtctctgagc gcggcattga ggtcgtcggc gacgcctccg agctcgaacg
     1081 catcgtggac caggtgctgg ccgaccacgc cgaggtggtc gcgaaggtga agtccggcaa
     1141 gacccagtcc aagggcttcc tggtcggtca ggtcatgaaa gccactcgcg gccaggccgt
     1201 accgcagatc gtcaacgaca tcatcgatcg tcggctcgga gtgtaacccg atacctgggg
     1261 ggaggcgtcg ggcgcccaag gatcggcgcc tgccccgtca tcggtaggta gagggcagcg
     1321 cggtgctgct gagcccaacc cgggcgggct tggccgcatc acctaacctg ggcgattcgg
     1381 cctgcgctgt gggacaggag gcccggctcc actcccgaga tgaccacgca tcgcatcctg
     1441 gctggggcgg ccgcggtggc cgtgcttgcg ctgctcgcac gcttgagcct gctctcctac
     1501 gggctctacg tgctgattct ggcgttgttg gcctccgagt tgctcgcccg gttctccctg
     1561 gaccgcatcg aatgcctgcg cgatgtccgg gtcgaccgcg ccaagatcgg cgaccaggtg
     1621 gtcgtctcca ccatcatcaa gaacaacaag cccatccctg tgccatggat gctggccgag
     1681 gacgctctcc cgaggcgcct gccggtgtcc ggagagacca cgcgtctgct cgtgctcggg
     1741 ccgtacggcg atgcccggct gcactactcg atcaccatgg cccatcgtgg ctaccaccag
     1801 atcggcccgg tcgtgctcga gtccggtgac ctgttcggct tcgttcggaa gttccgcgcc
     1861 gcgagaaagg cccactacat caccgttcag cccgacaccg tgccgatcct cagatacgac
     1921 gtggctagcc gccgccccat cggcgacatc aaggtgcgcc gcctgctcca cgacgacccc
     1981 tcccgtctcg cgggtgtccg cgaataccag cggggcgacc ccctcaaccg catccactgg
     2041 aaagccaccg ccaggaccgg cgcgcttcac agcaaagtct acgagcacac cacactggcg
     2101 ggggccatga tcgccatgga cttccatgga ccttcgttcg aaggcgacga ggaccgccag
     2161 ttcatccgtt ctgagatggc cgtgaaagtc gccgcctcac tcgcggcgta cctggtgagc
     2221 ctccggcagc aggtgggctt catcagcaac gggctcgatg cggccgaacg ggccaagcgg
     2281 gaagaggggc tcctcgatgc ccgctcccgc cgcgaggcgc gcaacttcgc cgccagccgc
     2341 aagggcgccg atcgcctccg acccgtcgag gtcgcggcac gcaagggcca cgatcaggcc
     2401 atggccgtcc tcgacgctct cgcccggctc gagctctccg acggttgccc cttcgagcag
     2461 atgctgctcg atgagcatac gcgcctgccg cgtgacctga ccttgctggt cgttgtccct
     2521 cgcatgaacc cgctgctcta ccaggtcctc gcccgcgtgc gcttcagcgg gttctccatc
     2581 atggtcttcc tggtcgacaa cgccgaacag gcccatgggg cgcgcggggc cctggcagca
     2641 cagggcattg ggttcatgca cgtggcccgg ctcgatgacc ttcagcatgt cgcccactcg
     2701 gggatctgat ccgggctggt ccgcactccg caatgatccc cgcttctcga agattcctcg
     2761 catccccact caggtacccc gagggccatg gcacaacagg ggtggagggg gcgatagagc
     2821 ccc
//
LOCUS       DAOSPL010000504         2823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35564_length_2823_cov_2.633309, whole genome shotgun sequence.
ACCESSION   DAOSPL010000504 DAOSPL010000000
VERSION     DAOSPL010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35564_length_2823_cov_2.633309"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PLQ54_09905"
     CDS             complement(<1..1064)
                     /locus_tag="PLQ54_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93615.1"
                     /translation="MQLHPRSITRERSGWFHATRWPALGLVLLLLPGCTGEDQNAPGM
                     LAADQALRNPQAFLAFIKLGMAVKIAECRFTPAQARALAEVAGREGPAIRADVDATLA
                     EANQAAAKLSDVAERVVVAEDPEDVLGNAMLELMATPGALPGATEGPPGAQPVDAFAE
                     RIFGMFAPEGEGAGIGPVLDKHAAAVEAILATMDGEQRVAVAGIAEGVERVLTMYAHG
                     AMPADEVVIDDAGRDEQGAGDELPADDPPMAGPHTAMEVAEPTDPILTCTNEVIRALG
                     FEDPEAEPWAIEAAQPIVEEFMALSDEERPPQVEAFARRLAVEISHGTEQLAQKVRRT
                     LLAIAGYQEAPGLLARVANR"
     gene            1164..1724
                     /locus_tag="PLQ54_09910"
     CDS             1164..1724
                     /locus_tag="PLQ54_09910"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOS93616.1"
                     /translation="MNHLLLIGFKGCGKSTVGRLVARRLGRRFIDLDTVIERLFHERG
                     LQRMGFRDVFCQVGEAEFRRLEREAARIVARWAREAPAPMVIALGGGSLVDDETRSLL
                     LPLGRFVYLRVPRNEAVERALSADTPAFLPGRNPVEELSEVYSLREPIYRACAHITVD
                     IGGLGRRAAADRVVGEFDRLATSGGA"
     gene            1724..2785
                     /gene="aroC"
                     /locus_tag="PLQ54_09915"
     CDS             1724..2785
                     /gene="aroC"
                     /locus_tag="PLQ54_09915"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOS93617.1"
                     /translation="MGGSIFGKAFRITTFGESHGKAVGVVVEGVTPGLPLTEADIQIE
                     LDRRKPGQSEVATPRQESDTVHILSGVFEGRATGCPIALVIWNADARPEAYRDLAEVF
                     RPGHADYTYHAKYGIRDHRGSGRASGRETAARVAAGAIAKKVLAPAGVTIRAYTLQIG
                     AVRAERVDLDEVDRNPVRCPDPSAAARMVEAIREARDQKDSLGGIVECRATGVPAGLG
                     EPVFDKLDADLAKAMLSLGAVKGIEFGAGFGAVTMRGSEHNDALTAEGFATNHAGGVL
                     GGISTGAELVFRIAVKPTSSIGREQVTVDLDGRERRIAIGGRHDPCICPRIVPVVEAM
                     AALVLVDHLKRQSALRGGA"
BASE COUNT          420 a          987 c          956 g          460 t
ORIGIN      
        1 tagcgattcg cgacccgggc cagaagcccg ggcgcctcct ggtaccccgc gatagccaac
       61 agcgtccgcc gaaccttctg ggcgagttgc tccgtgccat gactgatctc caccgccagg
      121 cgcctcgcga aagcctcaac ctgtggcggc cgctcctcgt cggacagagc catgaactcc
      181 tcgacgatcg gctgcgccgc ctcgatggcc caaggctcgg cctctggatc ctcgaacccg
      241 agcgcgcgga tgacctcgtt ggtgcaggtg aggatcgggt cggtgggctc cgccacctcc
      301 atcgccgtgt gcgggcctgc catcggcggg tcatcggcag gcagttcatc accggcgcct
      361 tgctcgtccc gccctgcgtc atcgatcacg acttcatccg ccggcatggc gccgtgagcg
      421 tacatggtga gaacacgctc caccccctcg gcgatgcctg ccacagccac cctctgctcg
      481 ccatccatgg tggccaggat cgcttcgacg gccgccgcgt gcttgtccag cacgggcccg
      541 atgcccgcac cctcgccttc gggcgcgaac atgccgaaga tgcgctcagc aaaggcgtcg
      601 acgggctgcg cgccgggcgg cccctccgtt gctccaggca gcgccccggg agtggccatc
      661 agctctagca tggcattgcc tagcacatct tctggatcct ccgccaccac taccctctcg
      721 gccacgtcgg atagcttggc ggcggcctgg ttggcctcgg ccagcgtggc gtccacgtca
      781 gcacggatcg ccgggccttc ccgtcccgcc acctcggcca gggctcgcgc ctgagccggt
      841 gtgaagcggc attcggcgat cttcaccgcc ataccgagct ttatgaaagc gaggaatgcc
      901 tgcggattcc gcaacgcctg atccgctgcc agcatgcccg gggcgttctg gtcctcacca
      961 gtgcagcccg ggagcagcag gagcacgagg ccgagggcag gccagcgggt agcgtggaac
     1021 cagccgctcc gctcgcgtgt aatactgcgg gggtggagct gcattcccat cacctcggcg
     1081 cggatgttct gagcctgcag gagacaagtt cgggcagcgg cgcctggcgc cctgcccgcg
     1141 cccagcggag gtgcccggtc agcgtgaacc acctgctgct cattggcttc aaggggtgcg
     1201 gcaaatcgac ggtcgggagg ctcgttgctc gccggctcgg ccgtcgcttc atcgatctcg
     1261 acaccgtgat cgagcgcctc ttccatgagc gcggccttca gcgcatgggc ttccgggacg
     1321 tgttctgcca ggtgggggag gccgagttcc gccggctgga gcgtgaggcc gcacgcatcg
     1381 tcgcgcgatg ggcgcgggag gcgccggcgc ccatggtgat cgccctcggc ggcggaagcc
     1441 tggtcgatga tgagacccga tccctactac tgccgctcgg ccgattcgtc tacctgcggg
     1501 ttccacgcaa cgaggctgtc gagcgggcac tctccgccga cacaccagcc ttcctgcccg
     1561 gtcgcaaccc cgtagaggag ctgtcggagg tatactcgct gcgcgagccg atctaccgtg
     1621 cgtgtgccca cataaccgtc gacatcggtg gcctgggacg ccgggcggcc gcggaccggg
     1681 tggtcggcga attcgaccgg ttggcgacct cggggggtgc ctgatgggcg gaagcatctt
     1741 cggcaaggcg ttccgcatca ccaccttcgg tgagtcccat ggcaaggccg tcggggtagt
     1801 tgtggagggc gtcacgccag gcctgccgct caccgaggcc gacatccaga ttgagctcga
     1861 ccgccgcaag cctgggcaga gcgaagtggc cacgccccgc caggagtccg ataccgtcca
     1921 catcctctcg ggcgtgttcg agggccgggc cactggctgc cccatcgcct tggtcatctg
     1981 gaatgcggat gccaggcctg aagcctatcg cgatctggcc gaggtgtttc gtcccggcca
     2041 tgcggactac acctaccacg ccaagtatgg catccgtgac caccgcggat caggccgggc
     2101 ctccggccgg gagacggccg cccgcgtggc cgccggagcc atcgccaaga aggtgcttgc
     2161 cccagccggg gtcactatcc gagcctacac cctgcagata ggcgccgtcc gtgcggagcg
     2221 ggtcgatctc gacgaggtcg accgcaaccc cgtgcgctgc ccggatccca gcgctgccgc
     2281 tcgaatggtc gaggccatcc gcgaggcgag ggaccagaag gactcgctcg gcggcatcgt
     2341 cgagtgccgg gccaccggcg tccccgcagg cctcggcgaa ccggtcttcg acaagctcga
     2401 cgctgatctc gccaaggcca tgctgtcgct cggggcggtg aaggggatcg agttcggcgc
     2461 tggtttcggt gcggtgacca tgcgaggctc ggagcacaac gacgcgctca cggctgaggg
     2521 gttcgcaacg aaccatgccg gcggcgtttt gggaggcatc tcgaccgggg ctgagctcgt
     2581 gttccgcatc gccgtcaagc cgacgtcgtc catcgggcgg gagcaggtca cggtggatct
     2641 cgacggccgc gagcggcgca tcgccatcgg gggccggcac gatccctgca tctgccctcg
     2701 aatcgtcccg gtggttgagg ccatggccgc actggtgctg gtggaccacc tcaagcgtca
     2761 gtctgccctg cgggggggcg cgtgatcccg ccccgaaggg agggccgcct gacgggtccg
     2821 agg
//
LOCUS       DAOSPL010000505         2822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35589_length_2822_cov_2.295988, whole genome shotgun sequence.
ACCESSION   DAOSPL010000505 DAOSPL010000000
VERSION     DAOSPL010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35589_length_2822_cov_2.295988"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PLQ54_09920"
     CDS             complement(<1..654)
                     /locus_tag="PLQ54_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93618.1"
                     /translation="MTTTDTTLTPINFADVKTGDRIRIEYQGECRVEDVVQTRNDYWL
                     DCSITSFPKRAGMKLYLLDRPDDEATLRAKRARLAYWEACGEPRHSEPKEASLNAWKA
                     ALGAADDDDAPTKPKPEPRRVEVGDKIRITRLSTDGFYSKTLQVGMVGTVTDDRPSCD
                     GYWAIQEVNFHPVRYGGYLIQDIRPGDFEIVTWKPIAGPDIKIGDYVRMTDRQANLVI
                     "
     gene            complement(651..911)
                     /locus_tag="PLQ54_09925"
     CDS             complement(651..911)
                     /locus_tag="PLQ54_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93619.1"
                     /translation="MRSSATQKQLDMPPITEAEIERFAQCLEKQAQEIDPRANALLAD
                     VDLYSAEVAKGALNLSDMQQGPAKRALNAINNKLHMILEGRR"
     gene            complement(904..1203)
                     /locus_tag="PLQ54_09930"
     CDS             complement(904..1203)
                     /locus_tag="PLQ54_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93620.1"
                     /translation="MQFIIHDRDDNNVEHFTSPEGLDALLSSEIVFGKLQGWHPTRMR
                     DLRDARKAIAGEDWDALYERLRSSGIAAYIIDGTIIHPAIELRAHDMDDEEHADA"
     gene            complement(1301..1573)
                     /locus_tag="PLQ54_09935"
     CDS             complement(1301..1573)
                     /locus_tag="PLQ54_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93621.1"
                     /translation="MTIQHQFEQFDRENPHVYDKLVGLAIEWLDQNASVGMKMLWERL
                     RWEFGVQTTEEAPRLNNNYTSRYARKLLADFPEWGGRIKTRELQAA"
     gene            complement(1570..2070)
                     /locus_tag="PLQ54_09940"
     CDS             complement(1570..2070)
                     /locus_tag="PLQ54_09940"
                     /inference="COORDINATES: protein motif:HMM:NF014520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HOS93622.1"
                     /translation="MSLASELEALEALERHRGAMEEVEREKAEQALLDEYRPSPEALR
                     ELAYADWAQKAAPRFAPGERAACEGHPHPDWWFSGLVAERAEASSLCAACPARQTCYT
                     ASELEDHGIWGGEARDSLRLDVLCPHGHSNWRIYPETGRRKCLTCRQEADRDRRARLK
                     ALKEAA"
     gene            complement(2067..2291)
                     /locus_tag="PLQ54_09945"
     CDS             complement(2067..2291)
                     /locus_tag="PLQ54_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014286313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOS93623.1"
                     /translation="MRRFAEAEAVPANPGVHALTAAQAADFLGISVRTLYRLRKDGGG
                     PIYAKFGTAVRYPVAALHAYIDESMEVSAA"
     gene            complement(2284..2487)
                     /locus_tag="PLQ54_09950"
     CDS             complement(2284..2487)
                     /locus_tag="PLQ54_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93624.1"
                     /translation="MREDEMTFEKRLISSLVTLAIEAGLGQARLADALGWEVEKVWEF
                     ESAGGTRTVADVLSYARVVLTSA"
     gene            complement(2480..2785)
                     /locus_tag="PLQ54_09955"
     CDS             complement(2480..2785)
                     /locus_tag="PLQ54_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93625.1"
                     /translation="MKTAMKTPTSVADLHASLTTSIPKYPTSSAERIELRFPQKLRAA
                     AAGEVFAACRPAFTEDDEDDLGYVLAQLAGWVLHGEAYDPDITFADELRRGEEDRRA"
BASE COUNT          416 a          872 c          963 g          571 t
ORIGIN      
        1 gatcaccaag ttcgcctgcc tgtccgtcat ccgaacatag tcgccgatct tgatgtcggg
       61 gcctgcgatg ggcttccagg tcacgatctc gaagtcaccg gggcggatgt cctggatgag
      121 atacccccca taccttacgg ggtggaagtt gacttcctgg atcgcccagt agccatcaca
      181 gctggggcga tcatcggtga ccgtgccgac catgccgacc tggagtgttt tcgagtagaa
      241 gccatcggtc gagagccgtg tgatgcggat cttgtcgccc acctccaccc gacgtggctc
      301 gggcttcggc ttggtcggcg catcatcgtc atcagcggcc cccagcgccg ccttccaggc
      361 gttcagggac gcctccttgg gctcggagtg gcggggctcc ccgcacgcct cccagtaggc
      421 cagccgtgcc cgcttggcgc gcagcgtggc ctcgtcatcc gggcgatcga gcaggtagag
      481 cttcatgcca gcgcgtttgg ggaacgatgt gatcgagcag tccagccagt agtcgttgcg
      541 agtctgaacg acatcctcca cgcggcactc accttggtac tcgatgcgga tgcggtcgcc
      601 ggtcttgacg tcggcgaagt tgatgggcgt gagggttgtg tcggtggtgg tcatcggcgt
      661 ccttccagga tcatgtggag cttgttgttg atggcgttga gagcccgctt ggcggggcct
      721 tgctgcatgt cggagaggtt gagggcaccc ttggcgacct cggcggagta gaggtcaacg
      781 tccgcgagga gggcgttggc tcgggggtcg atctcctggg cctgcttctc caggcattgg
      841 gcgaagcgct cgatctcggc ctcggtgatc ggtggcatgt ccagttgctt ctgagtggcg
      901 ctgctacgca tcggcgtgct cctcgtcgtc catgtcgtgc gcccggagct cgatggccgg
      961 gtggatgatc gtgccgtcga tgatgtaggc cgcaatgccg gagctgcgga ggcgctcgta
     1021 cagggcgtcc cagtcctcgc cagcgatggc cttccgggcg tcgcgcaggt cccgcatccg
     1081 ggtcgggtgc cacccctgca acttaccgaa gacgatctcg ctggagagga gggcgtccag
     1141 tccctcgggg ctggtgaagt gctcgacgtt gttgtcgtcg cggtcgtgaa tgatgaactg
     1201 catggtgata ctccttctgg tcagtggttg tccgtgtagt agttgaggta gagggcgccg
     1261 atgagcagcg ccgtgatggc gagggtgatg aggctgtcga tcacgcggcc tgcaactctc
     1321 gggtcttgat gcgcccgccc cactcaggga agtcagccag gagcttgcgg gcgtacctgg
     1381 acgtgtagtt gttgttcagg cggggcgctt cctcggtcgt ctggacgcca aactcccacc
     1441 ggagccgctc ccagagcatc ttcatgccga cgcttgcgtt ctggtcgagc cactcgatcg
     1501 cgaggccgac gagcttgtcg tagacgtgcg gattctcccg atcgaactgc tcgaactggt
     1561 gctggatggt catgctgcct ccttcagggc tttgagtcgg gctcggcggt ctcgatcggc
     1621 ctcctgccga catgtgaggc actttcggcg ccccgtttcg gggtagatcc gccagttgga
     1681 gtgcccatgc gggcacagga cgtctaggcg gagggaatcg cgtgcttcgc cgccccagat
     1741 gccgtggtcc tctagttcgg aggccgtgta gcaggtctgg cgtgccgggc aggcggcgca
     1801 gaggctagac gcctcggcac gctcagctac gaggccagag aaccaccagt cagggtgtgg
     1861 gtgcccttca caggctgccc gctcgccagg ggcgaagcgg ggtgctgctt tctgtgccca
     1921 gtcggcgtag gccagctcac gcagagcctc gggggatggt cggtactcgt caaggagggc
     1981 ttgttcggcc ttctctcgct cgacctcctc catcgcccct cggtgtcgct ccagggcctc
     2041 cagtgcttcg agttcggacg cgaggctcac gcggcactca cctccatgga ctcgtcaatg
     2101 taggcatgga gcgctgccac cgggtagcgg actgccgtgc caaacttggc gtagatgggg
     2161 cctccaccgt ccttgcggag gcggtacagg gttctgacgg agatccccag gaagtcagcg
     2221 gcctgcgctg ccgtcagggc gtgaacgcca gggttggccg ggactgcctc ggcttcagcg
     2281 aacctacgca ctggtcagca ccaccctcgc gtagctgagg acgtcagcca ccgtgcgagt
     2341 gccgcccgcg ctctcgaact cccacacctt ctcgacttcc cagcccaggg catcggccag
     2401 gcgcgcctgg cccaagccag cctcgatggc cagtgtcacg aggctggaaa tcaatcgctt
     2461 ctcgaaggtc atctcatcct cacgcacggc ggtcctcctc accacggcgc agctcgtcag
     2521 cgaacgtgat gtccgggtcg tacgcctccc cgtgcaggac ccagccggcg agctgagcca
     2581 ggacgtagcc caggtcgtcc tcatcgtcct cggtgaaggc tggccggcag gcggcgaaca
     2641 cctcgccagc ggcggcggcg cggagcttct gcgggaagcg cagctcgatc ctctcggcgc
     2701 tggaggtcgg gtacttgggg atcgaggtcg tcaggctggc gtgcaggtcg gcgacgctcg
     2761 tcggggtctt catggcggtc ttcatgtggc ttactccttc atgttggtat gagtgtgaca
     2821 tg
//
LOCUS       DAOSPL010000506         2820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35621_length_2820_cov_2.736347, whole genome shotgun sequence.
ACCESSION   DAOSPL010000506 DAOSPL010000000
VERSION     DAOSPL010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35621_length_2820_cov_2.736347"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2241)
                     /locus_tag="PLQ54_09960"
     CDS             complement(<1..2241)
                     /locus_tag="PLQ54_09960"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOS93626.1"
                     /translation="MDRLRWARSMGAAAVALLLVGTVTPTHAQGAVAEPSQAVKPSYR
                     VAGRTEVTVRVYDLSGVDAASRAQWRRTATPNAQAVAIEAADPDGSIERALLGLQGVR
                     LVGQGQVVCAGNRKTAIEVPDLRYGRGAAFVKRDGAGQLVVAHQDIDPGVVLEVWHGP
                     ASSGESPQADSLILQLRARVPAGRSLPGDPAVPPQLGPPQVFEAETRAQLSLGAIPQR
                     MVYCAELLPDGNGRSFVTVVEARPSAGTLGAPGAVVVSVPSPEPPDLARAIVDELAAL
                     SAYDAAKYLPEASAQRFVDLATRRGPLPHTAAAELSRLADAVRDRLRRTEQLQVAQLL
                     ARTEDNQRQIAELEAQTAANRARFEDDVRAARDRLAALQARLSEQTTGTATAGAAPGL
                     GSPSGTNAALAAEGAGGTVAQANTARFTRIYTVHAGDQPEKKVRVDVELGPNGQQAPP
                     RVSVWDPAANDWVEVDPNNPGEFADFVEKALQDAQGAQVFLAPGGGALNMQVGGPAQG
                     GIDVLRAPQVVAGNQGPAFELGRITALVEQAQKLAKRVQDGLAAAAPDVKTAAEGAAE
                     VSQILEQINQAVAKLTNQTAGWGPGGLILPPGLGPRFGQAWAAGIPRVIVEGKPRQFT
                     GIGAIVTSVEPADGGKPQMVIRQVMPNSPAEEAGLTEGDVIVAVDGQEVPAELAEGVK
                     RIVGPEGSVVKLTVRREGHEGLLEFEVTRQKVTFDPATLKQFDVPEPGEPAGPAPGAP
                     GAPPAAH"
     gene            complement(2246..>2820)
                     /locus_tag="PLQ54_09965"
     CDS             complement(2246..>2820)
                     /locus_tag="PLQ54_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93627.1"
                     /translation="WSLLLIACGALAGRLLVPPSTHGTVLAPVGASGPVAFAPTARPV
                     TDPPVDFDLGMPGVPAASGVAAMAVAKPSPVATPVGTERPDSGLAFVVRSGARSAVTP
                     RRAGSPAVRKPAPLPMPRPKLTVRITQPDGVTGSRTIIIQSDEGEILGPYSPGVVPVP
                     DVMPREGPTPTPKPEGRPEYLTALYTLGGA"
BASE COUNT          427 a         1015 c          955 g          423 t
ORIGIN      
        1 atgggctgcg ggaggcgcac ctggggcacc gggcgccggc ccggctggct cgccgggctc
       61 gggaacgtcg aactgcttga gtgtggccgg atcgaatgtc accttctggc gcgtcacctc
      121 gaactccagg agtccctcgt ggccctcgcg acggactgtg agcttcacca ccgaaccctc
      181 gggcccgacg atacgcttga cgccctctgc caactccgcg ggcacttcct ggccatcaac
      241 cgccacgatc acatcgccct cggtgagccc cgcctcctca gctgggctgt tcggcatgac
      301 ttgccggatc accatctgcg gcttgccgcc gtccgcaggc tccacgctcg tcacaatggc
      361 gccgataccc gtgaactgcc tgggcttgcc ctcgacgatg acccgaggta tccctgccgc
      421 ccaagcctgg ccgaaccgtg gacccagccc gggaggcagg atgaggccac cggggcccca
      481 cccggcggtc tgattggtca gcttggccac ggcctggttg atctgctcga gtatctgaga
      541 gacttctgca gcaccctcgg cggcggtctt cacatcaggc gccgcggcgg ccagaccgtc
      601 ctggaccctc ttggcaagct tctgtgcctg ctcgactagc gccgtaatgc gaccgagctc
      661 gaatgccggg ccctgatttc ccgcgacgac ctgtggcgcg cggaggacat cgatgccccc
      721 ctgggcggga ccgcccacct gcatgttgag cgcgccgcct cccggtgcca agaacacctg
      781 cgcgccctgg gcatcctgaa gagccttctc cacgaagtcg gcaaactcac cggggttgtt
      841 ggggtccacc tcgacccagt cgttggcggc ggggtcccag acgctcaccc ggggcggtgc
      901 ctgttgaccg ttcggcccca gctcgacatc gacccgcacc ttcttctccg gctgatcacc
      961 cgcatggacg gtgtagatcc gggtgaaacg cgcggtgttc gcctgggcca ccgtcccgcc
     1021 ggccccctcc gccgcgagtg cggcattcgt ccccgaggga gagcccagcc ccggagcagc
     1081 gcccgccgtg gcggtccccg tcgtctgttc actaagccgg gcctgaaggg cggccagccg
     1141 gtcgcgagcc gcacgcacgt cgtcctcaaa acgcgcccga ttcgccgccg tctgtgcctc
     1201 gagttccgcg atctgtcgtt ggttgtcctc ggtgcgcgcg aggagctggg ccacctgaag
     1261 ctgctcagtc cgcctgagtc ggtcgcgaac cgcatcggcc agccgactca gctcggcggc
     1321 cgcagtgtgg ggcagggggc ccctgcgggt cgccagatcg acgaaacgct gtgccgaggc
     1381 ttccggaagg tacttggcag cgtcgtacgc cgagagtgcg gcgagttcgt ccacgatggc
     1441 tcgcgccagg tccggaggct cgggactggg caccgacacc accactgccc ccggtgcgcc
     1501 aagggtgccc gccgaggggc gggcctccac gacagtcacg aacgaccggc cgttgccgtc
     1561 aggaagcagc tcggcacagt acaccatccg ctgtgggatg gcgcccaggg agagttgtgc
     1621 ccgtgtctcc gcctcaaaca cctggggtgg gcccagctga ggaggtacgg ccggatcccc
     1681 tggaagcgac cggccggcgg gcacgcgggc ccggagttga aggatcaggg aatcggcctg
     1741 cgggctctcg ccgctcgaag cgggaccgtg ccacacctcg agcacgacgc cggggtcaat
     1801 gtcctggtgt gcgacaacaa gctgcccggc gccatcgcgc ttcacgaagg ccgcgccgcg
     1861 gccatagcgg aggtcgggca cctcgatggc ggtctttcgg ttgccggcgc agaccacctg
     1921 gccctggccc acgagccgga cgccctgaag tcccaagagc gcgcgttcga tggacccgtc
     1981 gggatcggcg gcctcgatgg cgacggcttg tgcattcggg gtggccgttc gccgccactg
     2041 ggccctcgac gccgcatcga cacccgacag gtcatacacg cggaccgtga cttcggtacg
     2101 tcccgcaacg cgatatgatg gcttcaccgc ttgtgacggc tcggcaaccg ccccctgcgc
     2161 gtgggtgggt gtcaccgtac ccaccagcag cagggccacc gccgccgcgc ccatcgagcg
     2221 cgcccacctg agtcggtcca tgctctcacg cacctccgag agtgtacagg gccgtgaggt
     2281 actcgggacg gccctcaggc ttcggtgtgg gagtgggccc ctcgcgcggc attacatcag
     2341 ggacgggcac tacgccaggc gaatatggac caagtatctc gccctcatcg ctctggatga
     2401 tgatcgtgcg gctcccggtg accccatccg gctgggtgat gcgaaccgtc agcttgggac
     2461 gcggcatcgg cagcggcgcc ggcttccgaa ccgcgggcga acccgcgcgc cggggcgtca
     2521 cggccgagcg cgcgcccgaa cggaccacga aagcgaggcc gctatcgggg cgttccgtac
     2581 ccaccggggt cgccaccggg ctgggcttgg cgactgccat ggccgcgact ccggaggccg
     2641 cgggtacgcc gggcatcccg agatcgaagt cgactggcgg atcggtcacc ggccgggcgg
     2701 tcggggcaaa cgcgaccggc ccagacgcac cgaccggagc caacaccgtg ccgtgggtcg
     2761 agggagggac cagcagccgc ccggcaaggg ccccgcaggc gatcagcaga agcgaccatg
//
LOCUS       DAOSPL010000507         2817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35676_length_2817_cov_2.986966, whole genome shotgun sequence.
ACCESSION   DAOSPL010000507 DAOSPL010000000
VERSION     DAOSPL010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35676_length_2817_cov_2.986966"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1605)
                     /locus_tag="PLQ54_09970"
     CDS             complement(<1..1605)
                     /locus_tag="PLQ54_09970"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOS93628.1"
                     /translation="MSNSALDPRVRQFILPTRIVWTSERGVANAGNLLRDDDRVCILE
                     RNPGEEPGSILLDFGRELHGGIRIDVPTTSTGQPARVRVRFGESASEAMGTPNQDHTI
                     HDFDTLVAWMGHVEIGCTGFRFVRIDLLEEGVTISVRQVLAVFLFRPLEYKGSFECDD
                     ERLNAIWSTGAYTVHLCAQDYVWDGIKRDRLVWIGDLHPELRTICAVFGNIDVVPASL
                     DYARDHTPLPGWMNGISSYSLWWILCHHDWYMAQGDVAYLEAQRHYLVALLEQLRSQV
                     DESGCERLEGHRFLEWPTSRDPRAIDAGLQALVAMSLRAGASLCIALGEEDAAREATA
                     VANRAARYPREASETKQANALRVLAGMADPAEVNACILSQDPYRGLSPFYGYYVLQAR
                     AMAGDFVNCLDLIRTYWGAMIDLGATTFWEAFDMDWVEGAAGIADLVPRGKRDIHADC
                     GDFCYKGLRHSLCHGWAGGPTAWLTEHVLGLAPVAPGSRVVRLAPHLGGLAFARGTLP
                     TPHGIIRVAHEARGDGRIHSAIEVPAG"
     gene            1768..2535
                     /locus_tag="PLQ54_09975"
     CDS             1768..2535
                     /locus_tag="PLQ54_09975"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOS93629.1"
                     /translation="MDASPGPVPPADFVWPGGQRAALSLSFDDARPSQLDHGIPILDR
                     HGLRGTFYVSLGNLRDRIDEWRAAAATGHEMGNHTLSHPCSGNFPFNRDNALEKWTLE
                     RIEDDIDEAQELLEDLLGRRPRTFAYPCGQMYVGRGESVQSYVPVVARRFVVGRNAFD
                     EMHNDPGFCDLAQIYSMDADQGRLDRLIGLVEAAKRDGGWLVLMMHEVRPDSGRQTIS
                     AEVLDGLCRYLSGPAAEVWVDTVEAVGDYVRATRGGW"
     gene            2578..>2817
                     /locus_tag="PLQ54_09980"
     CDS             2578..>2817
                     /locus_tag="PLQ54_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93630.1"
                     /translation="MSDTRAQQRLALQGGLKAVSSIEGKGEPKIGVEKFMSVAERFGF
                     SEGTLAKIRQAVSEEDMGSGPFLANYYSDLPETKVQ"
BASE COUNT          497 a          899 c          959 g          462 t
ORIGIN      
        1 cccggcgggc acctcgatag ccgagtggat ccgtccgtcg cctcgggcct catgggccac
       61 gcggatgatc ccatggggtg ttgggagcgt gccccgtgcg aacgccagtc cgcccaggtg
      121 cggcgccagc cgaacgacgc gagagccggg ggcaaccggg gccaacccga gtacatgctc
      181 ggtgagccac gccgtcgggc cacccgccca gccgtggcag aggctgtggc gcaacccctt
      241 gtagcagaag tctccgcaat cggcgtggat atcgcgcttg ccgcgcggca cgaggtcggc
      301 gatgcccgcg gccccctcga cccagtccat gtcgaaggcc tcccagaagg tcgtggctcc
      361 gagatcgatc atggcgcccc agtaggtgcg gatcaggtcg aggcagttca cgaagtcgcc
      421 cgccatggcg cgcgcctgca agacgtagta gccgtagaac ggggacagcc ctcggtacgg
      481 atcttgggag aggatgcagg cgttgacctc ggccgggtcg gccatcccgg caagcacgcg
      541 cagtgcgttg gcttgtttgg tctcgctggc ttcacgcggg taccgtgcgg ctcggttggc
      601 gacggccgtc gcctcgcgcg cggcatcctc ctcgcccagg gcgatgcaca gcgaggcgcc
      661 ggcccgaagg ctcatcgcca ccagtgcctg caagccggcg tcgatggccc tgggatcccg
      721 gctggtgggc cactcgagga accggtgccc ctcgagtcgc tcacagcccg actcgtccac
      781 ctgcgaccga agctgctcga gcagcgcgac caggtagtgc ctctgggctt cgagataggc
      841 cacgtcgccc tgcgccatgt accagtcgtg atggcacaga atccaccaca gcgagtagga
      901 ggagatcccg ttcatccaac ccggaagtgg agtatgatcg cgggcatagt cgaggctggc
      961 ggggacgaca tcgatgttgc caaacacggc gcagatggtg cgcagctccg ggtgaaggtc
     1021 accgatccag accagccggt cgcgcttgat gccatcccac acatagtcct gggcgcacaa
     1081 gtgcaccgtg tatgcgcctg tgctccagat ggcgttcagc cgctcgtcat cgcactcgaa
     1141 gctccccttg tactccaggg gacggaacag gaaaacggcc agaacctgcc ggaccgagat
     1201 ggtgacgccc tcctccagca gatcgatgcg aacgaaccga aacccggtgc aaccgatctc
     1261 cacatgcccc atccaggcca cgagcgtgtc aaagtcgtgg atggtgtggt cctggttcgg
     1321 cgttcccatg gcctcgctgg cggattcgcc gaaccggaca cggacgcgcg ccggttggcc
     1381 ggtgctcgtt gtgggcacat cgatgcggat accgccatgg agttcgcggc cgaagtcgag
     1441 caggatcgag cccggctcct cccccgggtt gcgttcgaga atgcatacgc ggtcatcatc
     1501 acgcagcagg ttgcccgcgt tggcgacacc gcgctctgat gtccagacga tgcgcgtggg
     1561 caggatgaac tggcggaccc gagggtcgag agcggagttc gacatggcgg cagtcccctc
     1621 actgtgccga ttggggcacc ttcatggcgt cgcggccccg cgcggagtct ttccgtgcac
     1681 ggcaagcagg gattcggtcc tctaccggtc gaagcccttc tacgttacga tggccgtcac
     1741 cgaacatgcc gcgagaggga ttgtcgtatg gatgcgagtc ccgggccggt gccgccggcc
     1801 gactttgtgt ggccgggcgg ccaacgggcc gctttgagcc tgagcttcga cgatgcgcga
     1861 cccagtcagc tcgaccatgg gattccgatc ctcgaccgcc atgggctccg gggaacgttc
     1921 tatgtctcgc tgggcaacct gcgggaccgg atcgacgagt ggcgggcggc cgcggccacg
     1981 ggccatgaga tgggcaacca cacgctcagc cacccgtgca gcggcaactt ccccttcaat
     2041 cgggacaacg cgttggagaa atggactctg gaacggatcg aggatgacat cgacgaggcg
     2101 caggagctgc tggaggatct gctcggccgt cggccaagga ccttcgccta tccctgcggt
     2161 cagatgtatg tcggccgggg tgagagcgtt cagagctacg tgccggtggt cgcccggcgg
     2221 ttcgtcgtcg gacgcaacgc cttcgacgag atgcacaacg atcccgggtt ctgtgacctg
     2281 gcgcagatct acagcatgga cgccgatcag ggtcgcctcg accgactgat cgggttggtc
     2341 gaggcggcca aacgcgacgg cgggtggctg gtgctgatga tgcacgaagt ccggcccgac
     2401 agtggccgcc agaccatctc cgccgaggtg ctcgatggcc tctgccggta tctttccggt
     2461 ccggctgccg aagtgtgggt cgacacggtg gaggccgtgg gcgactatgt gagagcgact
     2521 cgaggcggct ggtaggccgc cagaccgaac gccgatgtag gaggacactc caaaccgatg
     2581 agtgacaccc gcgcgcaaca gagactggca ctccagggcg gcctgaaggc cgtctcgtcc
     2641 atcgagggca agggggagcc caagatcggt gtcgagaagt tcatgtccgt cgccgagaga
     2701 ttcggcttct ccgagggcac gttagcgaag atccgtcagg ccgtcagcga agaggacatg
     2761 ggatcggggc cgtttcttgc caactactac tccgatctgc ccgagaccaa ggtgcag
//
LOCUS       DAOSPL010000508         2814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35716_length_2814_cov_3.110910, whole genome shotgun sequence.
ACCESSION   DAOSPL010000508 DAOSPL010000000
VERSION     DAOSPL010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35716_length_2814_cov_3.110910"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2281)
                     /locus_tag="PLQ54_09985"
     CDS             complement(<1..2281)
                     /locus_tag="PLQ54_09985"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HOS93631.1"
                     /translation="MWARLPWAGIVAVAAGMSYATPAELVDILNGPVLDQRTPSVAAD
                     RHASVAPGVPVGQTIVTGPGSRELTRVAIWQAFWHESWQPDESLVLTLWDSPERKVSL
                     GRFAIPYARRMHEGAISLFDLDATVEPERAYYLELTVEVEPLRPAELPREWVLSGKRD
                     PAAGGDGVVGGIGTASGDYAAGTAYVGGEAQDYDLWFETHVRRERSADDLLGEAFGRF
                     DLSRPDLAELARAVVSKDWDAAASALVEHFERRTDLFPTPEPAPAGESPFDTREADLA
                     AEQKVLLADGTTVDLGPHWNHYALWPERGGVGLTRSGLRKPLAAGYQRTHNEKYARAF
                     VDMIRTQIARYPSPIRAGAYAPEETIPAALPCGIAGGSFWSGLAIGARMGHGFHYYAP
                     FVDSPYFTRDVRAAFIINLGEMAEVLEHMRGGGNWETQMSVGLFEFGLAYPEFVRARE
                     WVKQGFETVVENALSTVRPDGCLQEPAIGYHMMCMRRYARIIREAADLGLTVPDEMRT
                     LTERMYEYVMYCTLPDGTLPIWGDANPPMRPDDLVPDADLFGREDFRYVGSAGQEGTP
                     PAETSRGFADGGFYFQRSGWDSNAHYMGIRCGPFGSHGHFDALSMVVASYGKLRLIDP
                     GIHVYGTPEAKELMSTASHNTVSVDGADARSAVLHRWEARERLDYFAGHNEGYGGVEG
                     VTHHRRVLFVKPHGGRPGLWLVFDDIVGNGTHRADLHFRFAPSPMGADPNGSGVWTSD
                     ADANLLVRTVGAPLTLGEGI"
     gene            2485..>2814
                     /locus_tag="PLQ54_09990"
     CDS             2485..>2814
                     /locus_tag="PLQ54_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS93632.1"
                     /translation="MRPRPPGFTLIELLVVIAIIAILAAILFPVFAKAREKARQTSCL
                     SNLKQIGVALAMYRDDYDGVNCAYRFCASADPKPTSFYPPDVWWAPYDPSVQPDATPG
                     ANWQNGLL"
BASE COUNT          460 a         1035 c          879 g          440 t
ORIGIN      
        1 cgatcccctc acccagtgtc agcggcgcgc ccaccgtccg aaccagcagg ttcgcgtcgg
       61 cgtcggacgt ccacacgccg ctgccgttcg ggtcggcgcc catcgggctg ggggcgaacc
      121 ggaagtgcag gtcggctcgg tgagtcccgt tgccgacaat gtcatcgaac acaagccaca
      181 gccccggtcg cccgccgtgc ggcttcacga acagcacccg ccggtggtgc gtcacccctt
      241 cgaccccgcc atacccctcg ttgtgcccgg cgaagtagtc cagccgctcc cgggcctccc
      301 agcgatggag caccgcgctc cgcgcatccg cgccgtcgac cgagaccgtg ttgtgtgagg
      361 ccgtggacat gagctccttg gcctccggcg tcccgtagac gtggatgccc ggatcgatca
      421 gccgcagctt gccgtaactc gccacgacca tgctcagtgc gtcgaagtgc ccatggctgc
      481 cgaacgggcc gcagcggatg cccatgtagt gcgcgttcga gtcccacccg cttcgctgga
      541 agtagaaccc gccgtcggca aaccccctgg acgtctcggc tggcggcgtg ccctcctggc
      601 ccgccgaacc cacgtaccgg aagtcctccc ggccaaacag gtcggcatcg gggaccaggt
      661 catccgggcg cattggggga ttggcgtcgc cccagatcgg caaagtgcca tccggcagcg
      721 tgcagtacat cacatactca tacatgcgct cggtgagcgt ccgcatctca tcgggcaccg
      781 tgagtccgag gtcggctgct tcgcggatga tcctggcata gcgccgcatg cacatcatgt
      841 ggtagccgat agcgggctcc tgcaggcaac catccggacg caccgtcgag agagcgtttt
      901 cgaccaccgt ctcgaagccc tgcttcaccc actcgcgcgc ccgcacgaac tccggatagg
      961 ccaacccgaa ctcgaagagc ccgacggaca tctgggtctc ccagttgccc cctccccgca
     1021 tatgctcgag cacctcggcc atctcgccca ggttgatgat gaacgccgcc cgcacgtccc
     1081 gggtgaaata gggagagtcg acgaacgggg cgtagtagtg aaacccgtgc cccatgcggg
     1141 cgccaatggc gaggcccgac cagaagctcc cgccggcgat gccgcaaggc agcgctgcgg
     1201 ggatggtctc ctccggcgca taagcgcccg cccggatggg cgacgggtag cgcgcgatct
     1261 gcgtgcggat catgtcgaca aaggcgcgag cgtacttctc gttgtgcgtg cgctgatagc
     1321 ccgcggccag aggcttgcgt agtccgctgc gggtcagacc cactcccccg cgctccggcc
     1381 agagagcata gtggttccag tgggggccca ggtccaccgt cgtgccgtcg gcgagcagga
     1441 ccttctgttc ggccgccaga tccgcctcac gtgtgtcgaa tggcgactcg ccggccggcg
     1501 ccggttcggg cgtcgggaac aggtcggtgc gccgctcgaa gtgttccacc aacgcggacg
     1561 cggcggcgtc ccagtccttc gatacgacgg cccgtgccag ttccgccagg tccggccgcg
     1621 acagatcaaa gcggccgaaa gcttcaccca gcaggtcgtc cgcgctccgc tcgcgcctga
     1681 cgtgggtctc aaaccacagg tcatagtcct gggcctcgcc gcccacatag gcagtgccgg
     1741 ccgcgtagtc gccggacgcc gtcccgatgc cccccacgac gccgtctcct ccggcagccg
     1801 gatcgcgctt ccccgagagc acccactccc gtggcagctc cgccggtctc agtggctcga
     1861 cctcgaccgt gagctccagg tagtaggcgc gctcgggttc gaccgtcgcg tccagatcga
     1921 acaggctgat ggcgccctcg tgcatccgcc gcgcgtaggg gatcgcgaac cgccccagcg
     1981 agaccttgcg ctcggggctg tcccacagcg tgagcaccag cgactcgtcg ggctgccacg
     2041 actcgtgcca gaacgcctgc caaatggcga cacgagtcag ctctctggac cctggtccgg
     2101 tgacaatggt ctggcccacc ggcaccccgg gcgccaccga cgcgtgacgg tcggccgcca
     2161 ccgacggcgt ccgctgatcc agcaccggcc cgttgaggat gtcgacgagc tctgccggcg
     2221 tggcatagct catgccggcc gccacagcca cgatgcctgc ccacggaagc ctggcccaca
     2281 tgccaatccc tcccgccgcc gtaacgcgcc ccgccaaggt catcgcatgg cctcatcggc
     2341 cgcaactgcg tgtctgccgc ttcgggcgtc tcgcgtcgcg ccggccaaca gatatgtcag
     2401 acatgccgcc gaccgtgcga aggagacgga gagcttggag ccacggccaa atcaggccgg
     2461 gccttcagga tggaggtctt gcggatgcgg cccagaccac ctgggttcac gctgatcgag
     2521 ctgctcgtcg tcatcgccat tatcgccatt ctcgctgcca tactgttccc cgtattcgcg
     2581 aaagcccgcg agaaggcgcg gcagacctcg tgcctgtcga acctgaagca gatcggtgtc
     2641 gcgcttgcca tgtaccgcga cgactatgac ggcgtgaact gcgcctaccg attctgcgcg
     2701 agcgccgacc caaagcccac cagcttctac ccgcctgacg tctggtgggc cccctacgac
     2761 ccgtcggtcc agcccgacgc gacacccggc gccaactggc agaacgggct gctg
//
LOCUS       DAOSPL010000509         2813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35733_length_2813_cov_2.881436, whole genome shotgun sequence.
ACCESSION   DAOSPL010000509 DAOSPL010000000
VERSION     DAOSPL010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35733_length_2813_cov_2.881436"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /locus_tag="PLQ54_09995"
     CDS             complement(<1..1477)
                     /locus_tag="PLQ54_09995"
                     /inference="COORDINATES: protein motif:HMM:NF019463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93633.1"
                     /translation="MTGSVLTLEEAWELTGAMSSRRLVRAADIMASGEVVNTYSKVHP
                     IDGLVPRVPWAVYLAERGGGFRLILADFDQKTTGSATADAAQFVALLDELAIAHICCA
                     SSGSPEGGRHVWIALADSLDAARTAHLAHLLQSCWTTFDPTPLLNPATGAGRPPGAPH
                     RHGGQSEVLAGDPTILTRPTTTSADIAVLLGRLADRARVVEAQGHVGVARTTLDDGAA
                     PRLGGARRPLSAVIRGLVDAVPAGDASAALWAILCGAARASWSLGEVAELLPRPGLEH
                     ARTKRHADGQRLPRPSAGSNAPHAVLDRMWRRAVAYVAAHPATGADPTFEARAGAVTQ
                     LAWDLQRYADVSPGRWGSTRGVTDRLVLDAVTKLAVDAVKPEVEASIRTIAEIVGIDR
                     EAVRCALIRLVNEGWLTRTRATAGRRAAYYSIDRNNCFHSLVERFLSQADAPPTRRAT
                     LQSTLTTRLGRASHDTFAPRAGLGRTAGLLYARLHEQDRTST"
BASE COUNT          434 a          904 c         1029 g          446 t
ORIGIN      
        1 tcgttgaggt gcgatcttgc tcgtggaggc gcgcgtagag cagtccggcg gtacgtccaa
       61 gccccgcacg gggggcgaaa gtgtcgtgcg aggcgcggcc gagccgggtg gtcagggtgg
      121 actggagggt ggcccgccgg gtggggggcg catctgcttg tgacaggaat ctctcgacaa
      181 ggctgtggaa acagttgttc cggtcgatgg agtagtaggc ggcacggcgg ccggccgtgg
      241 cgcgggttcg ggtcaaccag ccctcgttga cgagcctgat gagggcacac ctgacggctt
      301 cgcggtcgat gccgacgatc tcggcgatgg tgcggatcga cgcctctacc tcgggcttca
      361 cggcgtccac tgcaagcttg gtgacggcgt ccaggacgag ccggtcggtg acaccgcggg
      421 ttgagcccca gcgtccgggg ctgacatcgg cgtaccgctg cagatcccag gcgagctggg
      481 tgacagcgcc ggcgcgtgcc tcgaaggtgg gatcggcgcc ggtagcggga tgcgcggcca
      541 cgtaggcgac agcacggcgc cacatgcgat ccaagacggc gtggggcgcg ttgctgccgg
      601 cgctcgggcg ggggagacgc tgaccgtcgg cgtgccgttt ggtgcgggca tgttccaggc
      661 cggggcgggg gagtagctcg gcgacctcgc cgaggctcca ggacgcccgg gcagctccac
      721 acaggatggc ccacagcgcc gccgacgcgt cgcccgccgg tacggcgtcc accaggcccc
      781 tgattactgc ggacagcggt cgtctggcgc cgccgaggcg tggcgctgcg ccatcgtcaa
      841 gcgtggttcg ggccacgccc acgtgccctt gggcttcgac cacccgagct cgatcggcga
      901 gtcgtcccag caggacggcg atgtcggccg acgtcgttgt gggccgggtc aggatcgtcg
      961 ggtcgccggc gagaacttca gactggcccc cgtggcggtg gggcgcgccg ggcggccgac
     1021 cggctccggt ggccgggttc agcagggggg tggggtcgaa ggttgtccag cagctttgca
     1081 gcaggtgtgc caggtgcgcg gtcctggctg cgtccaggga gtcagcgagc gcgatccaca
     1141 catggcggcc accctcaggg gacccggacg aggcgcagca gatgtgcgcg attgcgagct
     1201 cgtcgaggag cgccacgaac tgcgcagcgt cggcggttgc ggagcctgtt gtcttctggt
     1261 cgaagtcggc caagatcagt cggaaacccc cgccacgctc agccaggtac accgcccacg
     1321 gcacccgcgg caccagcccg tcgatcgggt ggaccttcga gtaggtgttg accacctcgc
     1381 ccgacgccat gatgtcggcc gcccgcacca gccggcgcga gctcatcgcc cccgtcagct
     1441 cccacgcctc ctcgagcgtc aacaccgatc cggtcacgcc gccgccgata cacgctgcgg
     1501 tgcggtcagt gccgcaacca gatctgccct gccagcgcgg tacaagcggc ggcggagcgc
     1561 ctgtgaggag gcgcagccca accgacgggc gatcgacccc tcggtgtccc agcctgctag
     1621 ccattccacc tcggccaaga acaacgcgcg ggcggcattc cggcgggcga tgtactccga
     1681 gctcacgtga ggcgccgcag cagccaccgg ttgaatacgg ggagcgcagg ctccgcacac
     1741 acccaacgga tcaaccatct ggctaggggt tcgccactgg ttgcagcgcg ggcacaggga
     1801 tgcgtgcgga tgacgagatg ccttcaccac accgcagcga ggcgccgcgc agtgcggcgt
     1861 gcctcttgcc acctgagacc aggtacctgt tacagttctg gacgtagttg aacctccctt
     1921 ccgggctgcg aattcgaatc gggggctata cccagatcga ggactcttga ccaagtcgtc
     1981 gatcaccact cttagaagcg ggctggtgct ggtaacaccg gcccgcttcg tgtttaaccc
     2041 gtcatgggaa gttcctcctg acggcggggg actcgaccgg ggcgtccggg aggcgggtcg
     2101 gcgatgccaa acaagcgcca tctagcaagt gactgcacgc tgatgccatg gccggcggct
     2161 tcctctttca gagcagcctt ctgggccttc gacatgcgga cgagaatcgc gtcgtctgct
     2221 cgagtggaca tgagtccgag cctatggggg tgatatcgcg atataggcca cctggcggac
     2281 gacacgccga gccgacctcg acagggccca gcgctggcgc ggctgtgttg cacgatcgat
     2341 gctcatcggc tgacccccgt gcggatcgcg acgccgcgcg tagacaccag tgtcgggccg
     2401 atcccacgat gaagttcggc gttcggggtc gaccgggttg ggcgttcggt ggcgctgtga
     2461 ctgggggggt gggcaaaggc gatgaatggc gcgcccgccc tcgactggag ggctaggtgc
     2521 gcggttgcgg tcgccatcct tgccctctcg gattggcgga ggagaggcat cgcggcctct
     2581 cggctgaact ggtcgaggac gacccagccg ccgtgccttc cattgtgagc ggaggccccc
     2641 cagagtcgtc gactgccgtg gaccagagag tcgatggcct ggaggtagtg gtcggcaact
     2701 tcggcagtgg tccggttgac gcggcggacc aggttgagga ggtcggcggg ggagcgctgc
     2761 gccaccagct cctcgcccgt tacccagcgg ccctcgggtc gcatgagggc ctt
//
LOCUS       DAOSPL010000510         2812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35745_length_2812_cov_4.155604, whole genome shotgun sequence.
ACCESSION   DAOSPL010000510 DAOSPL010000000
VERSION     DAOSPL010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35745_length_2812_cov_4.155604"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1748)
                     /locus_tag="PLQ54_10000"
     CDS             complement(<1..1748)
                     /locus_tag="PLQ54_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93634.1"
                     /translation="MRFAAVLLVVALPCPTSAERSDSVDPARPEPLQISGIYPHLAVF
                     NQPDDVADRTNHGECGTGAIVPWVGKLWVITYPQHKVTGSNDKLYEIAPDMSVTIRPE
                     SVGGTHACRMIHRESNQLIIGPYFIDAYGRVRSVDLQQLRGRMTAVMRHLTDPANKVY
                     YYDMEGALYEVDVHTLAVRLLFAKPVPGWHGKGGYTAQGRVVIANNGESGGTRGYDQL
                     LVGGPARGDEAGVLAEWDGREWRIVERAKFTDVTGPGGISGSPSDDSPLWAVGWDKRS
                     VVLKLLDGGEWHTFRLPKASHSFDPDHGWYTEWPRIREIAPGRPMLCMHGGMFDFPSG
                     FRAADTSGIRPIGIHLRYIPDFCYWGHRVVLGADDASMMQNPMCGQGQSNLWFGHEEE
                     LAHFGPRAGWGGVWLGDEVEAGQPSDPFLIAGYDQRVLHLANDGSEPVRFNAEVDLDG
                     RGQWVPLASYQVPVAGYRSAVLPEDLKAEWIRVTPSIDCRATAYLHCWSPRQGEDGEE
                     RLFDGLADADGARDYTGGIIRPAAHNRSLQWWARDVERSGTAMRPRYVEVNLSPEGTL
                     SFELPVFIYFLTKVGVV"
     gene            complement(1761..>2812)
                     /locus_tag="PLQ54_10005"
     CDS             complement(1761..>2812)
                     /locus_tag="PLQ54_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative collagen-binding domain-containing
                     protein"
                     /protein_id="HOS93635.1"
                     /translation="GAGPDTGGGWINGRGDDEMVLFRGYQHIVSFFTSMPWWELEPDN
                     DFLLEGSDSPVRTELSHIVYTRDAEGNATLYVDGEPVATGAVTGDPSSWDVSYRLALA
                     NELTRDRTWLGEYRRVAIYDRALSADQVRAAAGAGAEGAILRYEFDDGGGDLVHDTSG
                     IGEPLDLRIADAGTVEWLPGGGLAVRQSALIASEGPAVKVIEAIKASGAFTLEALVKP
                     ANTMQAGPARIVTLSRDTGHRNFTLGQAAAAYEVRFRTTNTSPNGEPSVATPGAPNAL
                     PRAMGMRSPAGDLAAVYLPAGGSVRVKPGALTDGISARWLNPRTGEWTDATPDADGSY
                     HAPDGDDWALRFAAG"
BASE COUNT          445 a         1005 c          911 g          451 t
ORIGIN      
        1 acgacgccca ccttcgtgag gaagtagatg aagacgggca gctcgaagga aagcgttccc
       61 tccgggctga gattcacctc gacatagcgg gggcgcattg ctgtcccgct gcgctccacg
      121 tcgcgggccc accactgtag ggatcgattg tgcgcggccg ggcggatgat gccgccggtg
      181 tagtcccgcg caccatccgc gtccgccagg ccgtcgaaga gccgctcctc cccgtcctcg
      241 ccctgccgcg gagaccagca atgtaggtag gccgtagccc ggcagtcgat gctaggggtg
      301 acccgaatcc actcggcttt caggtcttcg ggaagcacgg cggaacggta cccggcgaca
      361 ggcacctggt atgaggccag cggcacccac tggccccgtc cgtcgaggtc aacctcggca
      421 ttgaaacgaa ccggctcact accatcgttc gccaggtgaa gcacgcgctg gtcgtagccc
      481 gcgatgagga atgggtcgga tggctgcccg gcctcgacct cgtcgcctag ccacacacct
      541 ccccagccgg cgcgtgggcc gaagtgggcc agctcctcct cgtggccgaa ccacaggttc
      601 gactgcccct ggccgcacat ggggttctgc atcatcgagg catcgtcggc cccgagcacc
      661 acgcggtggc cccagtagca gaagtcgggg atgtaacgca gatggatacc tatggggcgg
      721 atcccggacg tgtctgccgc gcgaaagccg cttgggaagt cgaacatccc gccgtgcata
      781 cacaacatgg gccggccggg cgcgatctcg cggatgcgag gccactcggt gtaccaccca
      841 tgatcgggat cgaagctatg gctggccttc ggcagccgga aggtgtgcca ctctccgccg
      901 tcgagcagct tgagcacgac cgagcgcttg tcccacccga cggcccatag cgggctgtcg
      961 tcgctgggac tacccgagat gccgcccggc ccggtcacat cggtgaactt ggcccgttcg
     1021 acgatccgcc actcgcgccc atcccactca gccagcacac cggcctcgtc gccgcgcgcc
     1081 ggaccgccca cgaggagctg gtcgtagccc cgcgtgccgc ccgactcgcc gttgttcgcg
     1141 atcaccaccc gcccctgcgc cgtgtagccg cctttcccat gccacccggg caccggcttg
     1201 gcgaagagca accgcacggc cagcgtgtgc acatcgacct cgtagagggc accctccatg
     1261 tcgtagtagt agaccttgtt cgccgggtcg gtcaggtggc gcatcactgc ggtcatgcgg
     1321 ccgcgaagct gctgcaggtc gacgctgcgc acgcgcccgt aggcgtcgat gaagtagggg
     1381 ccgatgatga gctggttcga ctcacggtgg atcatccggc aggcgtgggt cccgccgacg
     1441 ctctcgggcc ggatcgtcac gctcatgtcc ggcgcgatct cgtagagctt gtcgttgctg
     1501 cccgtgacct tgtgctgggg ataggtgatg acccacagct tcccgaccca aggcacgatg
     1561 gcgcccgtgc cgcactcgcc gtggttcgtc cggtcggcca catcgtcggg ctgattgaag
     1621 accgccagat gagggtagat gccgctgatc tgcagtggct cgggcctcgc cgggtccacg
     1681 gagtcgctcc gttcggcact ggtgggacag ggaagcgcga cgacaagcag taccgcagcg
     1741 aaccgcacgg gcacgccctc ctatcctgcc gcgaaacgca gcgcccagtc atcgccgtcg
     1801 ggcgcgtggt agctgccgtc cgcatcgggc gtggcgtccg tccactcgcc ggttcgaggg
     1861 ttgagccagc gagcagagat gccgtctgtc agggcaccgg gcttcacgcg cacggaaccg
     1921 ccggccggga ggtacaccgc ggccaagtca ccggccggac tgcgcatgcc catggcccgt
     1981 ggcaacgcgt tgggggcgcc cggcgtcgcg accgaaggct ccccgttcgg cgacgtgttc
     2041 gtcgtccgga accgcacctc ataggcggcc gcagcctgcc ccagtgtgaa gttccgatgc
     2101 cccgtgtccc ttgacagcgt gacgatccgc gccggccccg cctgcatcgt gttggccggc
     2161 ttcaccagcg cctcgagtgt gaacgcgccg gaagccttga tggcctcgat caccttcacc
     2221 gccggcccct ccgacgcgat caaggcgctc tgcctgacgg ccagaccgcc gcccggcagc
     2281 cactcgacgg tcccggcgtc ggcgatacgc aggtcgaggg gctcgccaat acccgaggtg
     2341 tcgtggacga gatcgccgcc cccgtcatcg aactcatacc gcaagatcgc gccttcagcc
     2401 ccggctccgg cggccgcccg cacctggtcc gcgctcaacg cacggtcgta gatcgccacc
     2461 cggcggtact cgccgagcca cgttcggtcc cgcgtcagct cgttcgccag ggcgagccga
     2521 tatgagacat cccaactcga tgggtcgccg gtcaccgctc ccgtcgctac gggttcgcca
     2581 tcgacgtaga gcgtcgcgtt gccctcggca tcgcgcgtgt agacgatgtg cgacagctcc
     2641 gtgcggaccg ggctgtcgct gccctcgagc aggaagtcgt tgtccggctc gagctcccac
     2701 cagggcatcg aggtgaagaa gctcacaatg tgctggtatc ctcggaagag caccatctcg
     2761 tcatcgccgc ggccgttaat ccagccgccc cccgtatcgg gtccggcccc gg
//
LOCUS       DAOSPL010000511         2811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35761_length_2811_cov_3.706821, whole genome shotgun sequence.
ACCESSION   DAOSPL010000511 DAOSPL010000000
VERSION     DAOSPL010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35761_length_2811_cov_3.706821"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLQ54_10010"
     CDS             <1..240
                     /locus_tag="PLQ54_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93636.1"
                     /translation="GIARAVRYWDGGKHSVYAQLDYMDPDRNTANDTQAGPVVGYIRK
                     LSPDTKVTFELNGRQNDGTAATDFAAGVRYQIEWD"
     gene            324..1196
                     /gene="modA"
                     /locus_tag="PLQ54_10015"
     CDS             324..1196
                     /gene="modA"
                     /locus_tag="PLQ54_10015"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOS93637.1"
                     /translation="MRREWVPGVWAAAAVAVTLMWGCHRQQPADTGAEAAPPKGASTA
                     AAPAKAPEAKSITVFAGAAAMAALKELGPMYETKTGIKVDITSGGSGSVMTQFVQEQF
                     GDVYMPGSDDFMDKAEAKDAVLKDTRAILVYLVPAICISKGNPKGIKSLDDLARADLR
                     VVLGEEKAVCLGDIAAAVLKEKGLAEKVQPRVASYASSCEDVLNKLLLGEADVVIGWD
                     SFAKQNPDKVETIPIQAESARVRHIPAAVIKWSTQRDAAKAFIDFLTSPDAMKVWEAR
                     GYTTKAPESVSKGT"
     gene            1196..2068
                     /gene="modB"
                     /locus_tag="PLQ54_10020"
     CDS             1196..2068
                     /gene="modB"
                     /locus_tag="PLQ54_10020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HOS93638.1"
                     /translation="MGDAYVPNGSGSPGGAQAAEASGTGRLRSGARSTRWGLVAVTGV
                     FLALTAAPLLGLFAYAPCSMVFCPATVVILRKTLALTVSSSLTATLAAVIVGLPTALC
                     LARLSFRGRTVANALVELPISLPPLVLGVALLLVWGRRGVIGSWLSSHGYHLSFTFAA
                     VIIAQFVVAAPFFVRIAKAAIEQVPVSLEEASRTLGRGPIGTYLRVTLPLAQRGILAG
                     VITCWARAMSEFGATIMFAGNFPGRTQTVPLAIFSTMQYDTVTSVSLSIFMLAFSACT
                     FVLAQTLLGRSAAR"
     gene            complement(2152..>2811)
                     /locus_tag="PLQ54_10025"
     CDS             complement(2152..>2811)
                     /locus_tag="PLQ54_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93639.1"
                     /translation="LTTHARKGQPPIQPLDTMLLYERSDDSQVQAGTHEVLSLIHEEK
                     GPNSFPWTIYASLDTHHDAGDACVLCSRLHKHGPGWSTGLHSEVFNHGRAVALGANIE
                     MSSDYAGAEPTQVIGLNIQAVGGPTPMQYGIQIHEGQGRFEKGIGLNGSGQVGLDIAG
                     RYEAGIDTHGNDIRLAEGACVVLDGEGRIRLRYREGRIEFLNGDRCFAHLDVDAEDHA
                     V"
BASE COUNT          449 a          958 c          902 g          502 t
ORIGIN      
        1 gggatcgcgc gcgccgtccg ctattgggat ggcggcaagc attccgtgta tgcccaactc
       61 gactacatgg accccgatcg gaacacggcc aacgatacgc aggccggacc cgtcgtgggg
      121 tacatccgca agctgagccc ggacacgaag gtcacgttcg aactgaatgg gcgccagaac
      181 gacggtaccg ccgccacgga cttcgcggcc ggtgtgcggt accagatcga gtgggactag
      241 tcaccggtgt gcagacacgg cgtgtgtgtg tggcccggcc gtgctcgatg atggttggtg
      301 ggagaacgtg aggaggagag gcaatgcgac gtgagtgggt gcccggggtc tgggcagccg
      361 cggctgtggc cgtgacgctg atgtgggggt gccatcgcca gcagccagcg gacactgggg
      421 ctgaggcggc gcctccgaag ggggcttcca cggcggctgc gccggcgaaa gctcccgaag
      481 ccaagtccat cacggtcttc gccggcgcgg cagcgatggc ggccctcaag gaactcggac
      541 ctatgtacga gaccaagacc ggtatcaagg tcgacatcac gtcgggcggc tccgggtccg
      601 tcatgaccca gttcgtccag gagcagtttg gcgatgtgta catgcccggg tcggatgact
      661 tcatggacaa ggccgaggcc aaggatgcag tcctcaagga cacgcgagcc atcctcgtct
      721 acctcgtccc ggccatctgc atctccaagg gcaaccccaa gggcatcaaa agtctcgacg
      781 acctggcccg ggccgatctc cgagtcgtgc tcggcgagga gaaggccgtc tgcctgggtg
      841 acatcgccgc ggcagtgctc aaggagaagg ggctggcgga gaaggtccag ccaagagtcg
      901 cttcctacgc cagctcttgc gaggatgttc tgaacaagct tctgctcggc gaagcggacg
      961 tcgttatcgg ctgggattcc ttcgccaagc agaaccccga caaggtagag acgatcccca
     1021 tacaggccga gagcgcccgg gtccgtcata tcccggcggc ggtgatcaag tggtcaaccc
     1081 agcgcgatgc cgccaaagcc ttcatcgact tcctgacgtc cccggacgcc atgaaggtct
     1141 gggaggcccg cggctacacg accaaggcgc ctgagtcggt gtcgaaaggc acctgatggg
     1201 tgacgcgtac gtcccgaacg gcagcggttc accgggcgga gcgcaagcgg ccgaagccag
     1261 tggcaccggc cgcttgcgct cgggtgctcg atccacccgg tgggggctcg tggcggtaac
     1321 gggcgtattc cttgccctaa ccgccgcccc gctccttgga ttgttcgcct atgcgccgtg
     1381 ctccatggtg ttctgccccg ccaccgtggt catcctgagg aagacgctgg ccctgacggt
     1441 ctcgagtagc ctcaccgcca ccctcgcggc agtcatcgtg ggcttgccca ccgccctatg
     1501 ccttgcccgc ctgtccttcc gtggtcgcac cgtcgccaat gccctggtgg aactgccgat
     1561 ctcgctgcct ccgctggttc tgggcgtggc gttgctcctc gtctggggac gccgcggggt
     1621 cattgggtcg tggctgagca gccatggcta ccacctctcg ttcacatttg ccgccgtcat
     1681 catcgcccag ttcgtggtgg ccgctccttt ctttgtccgc atcgccaaag ccgccatcga
     1741 gcaggtgccc gtctccctcg aggaggcatc tcggacactc gggagagggc ccatcggcac
     1801 ctacctgcgc gtcacgctcc ccctcgccca gcgcgggatt ctggccgggg tcatcacctg
     1861 ctgggcccgc gcgatgagcg agttcggcgc cacgatcatg ttcgccggta acttcccggg
     1921 ccgaacgcag acggttcccc tcgcgatctt ctcaaccatg cagtacgaca ccgtgacctc
     1981 cgtcagcctg tcgatcttca tgctggcctt ctcggcctgc accttcgttc tcgctcagac
     2041 gctcctcggc cgcagtgcgg cgcgataggc gcgtccacgg ccttagcccc atcgctcctg
     2101 agccgccgcg ccatccgcgg cgtgacgaag ggcgaccggg cgcactcacc cctacacggc
     2161 gtggtcttcc gcatccacat ccaggtgcgc gaagcaccga tcgccgttga ggaactcgat
     2221 gcgcccctcg cggtaccgca gccggatccg cccctcgcca tccagcacca cgcaagcgcc
     2281 ctcggcgagg cggatgtcgt tcccgtgcgt gtcgatgccc gcctcgtagc gaccggcgat
     2341 gtccagcccc acctgccccg agccgttgag gccgatgccc ttctcaaagc gcccctggcc
     2401 ctcgtggatc tggatgccgt actgcatcgg cgtgggcccg cccacggcct ggatgttcag
     2461 cccgatgacc tgcgtcggct ccgcaccggc gtagtcgctg ctcatctcga tgttggcgcc
     2521 gagagccacg gctcgtccgt ggttgaacac ctcggagtgc aagcccgtcg accagcccgg
     2581 cccatgcttg tgcaggcgcg agcagagcac acaggcgtcg cccgcatcgt ggtgcgtgtc
     2641 cagactggcg tagatggtcc acgggaacga attcggcccc ttctcctcgt ggatcagcga
     2701 gagcacctcg tgtgtccctg cctgcacctg gctgtcatcg gaccgctcat agagcagcat
     2761 tgtatccagc ggttggatgg gtggttgccc cttgcgtgcg tgagtcgtca g
//
LOCUS       DAOSPL010000512         2811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35763_length_2811_cov_3.264877, whole genome shotgun sequence.
ACCESSION   DAOSPL010000512 DAOSPL010000000
VERSION     DAOSPL010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35763_length_2811_cov_3.264877"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="PLQ54_10030"
     CDS             <1..806
                     /locus_tag="PLQ54_10030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOS93640.1"
                     /translation="LGAVRQASKPPDLDHPYGHGKFESLSGAIEAGLIAMAAAGIVWS
                     AVRRLRGGGEIESVGAGLAVMAVSATMNALVSAHLLRVAKRHQSIALEADGWHLRTDV
                     YTSLGVLVGLALVGITGQKLLDPIAALVVAGVILHAAYSLTRKAVRQLLDSPVDPQIE
                     SLIANIVHTHSGQFVEAHKLRTRRAGAEIHVDLHLVVCPDMHVSLAHDLASHIEDDIH
                     AQVPGATAMIHIEGPEPGWAPLATGDEPPFCVRECDAATDDECPRARRP"
     gene            complement(819..1808)
                     /locus_tag="PLQ54_10035"
     CDS             complement(819..1808)
                     /locus_tag="PLQ54_10035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOS93641.1"
                     /translation="MRWGVDHPMTPEFESGATPVSDHPTADALPGPETPWGSAAKPPC
                     GNWIHAVVLLGGTAGLAYIYRRFFMGLPVAAGVAFDIASAALLVLYCAQALDIPLRSP
                     EIEALQVMLVAVISGRYLGQLVHVTSIGSQVLWLRPTGPRDVLILNWLTGASAFLVLI
                     PFAVILLIRRRRLSDYGLCWGDWRRWGPWVCGGVAIMIPILWFATGAFRELQTFYPMV
                     RGARASLGAFVVFQCFVVLLMFGWEFSLRGYLQFALQARLGLMAVVVQNVPFVLGHVR
                     KPESELYGTLVSGLFLGYIAYRGRSMLPALITHVAVAVINDAMVIAHLGGFRH"
     gene            complement(1792..>2811)
                     /locus_tag="PLQ54_10040"
     CDS             complement(1792..>2811)
                     /locus_tag="PLQ54_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93642.1"
                     /translation="TCHWCYAALPHAQNVADPRRAVVARPPQEWPLPHECRRCGGPLP
                     PRAFDCPRCGFSMLVDLPAASRPSKRGPWTWLGALVVLGVLAAPVAIVLRPRSESQAS
                     PIPQGQWHSPAAADLGFGSNTARPLVPTPADVTTGRDSPFPAPLPSTSIPATAQAYAS
                     PAVLKFLGANSQVDWAAELASWICEPPESLIVLPRESPDGAPLELADLGTLRSLAYPD
                     ERAVSGGSGTRGHGGSEASYHAHAHTIYVHKPSDVVTAFYQRLLGDNTRQTAWVTERF
                     IPVCQLVRIADREAVVVTVCPGSAFASAEPLAAIRLCRVERRPGAAGEYGENTGKGQP
                     REVGR"
BASE COUNT          517 a          918 c          989 g          387 t
ORIGIN      
        1 ccctgggtgc cgtgcgccag gccagcaaac cgcccgacct cgaccacccc tacggtcatg
       61 gcaagttcga gagcctctct ggcgcgatcg aggccgggct catcgcgatg gccgcggcgg
      121 gcatcgtctg gagcgcagtt aggcgcctcc gcggcggggg cgagatcgag tcggtgggcg
      181 ctggcctggc tgtgatggcg gtctcggcca cgatgaacgc actggtctcg gctcatctgt
      241 tgcgggtcgc gaaacggcac cagtcgatcg cgctggaggc ggatggatgg cacctgcgta
      301 ccgatgtcta cacatcgctg ggcgtgctgg ttggcctggc gctggtgggc atcacaggcc
      361 agaaactgct ggaccccatc gcggcgctgg tcgtggcggg cgtgatcctg cacgcggcct
      421 actcgctgac ccgcaaggcg gtgaggcagc tcctcgacag cccggtggac ccccagatcg
      481 agagtctgat tgccaacatt gtccacaccc acagcgggca gtttgtggag gctcacaaac
      541 tacgcactcg ccgcgcggga gcggagatcc acgtggacct gcacctggtc gtctgtcccg
      601 acatgcacgt ctccctcgcg cacgacttgg cttcgcacat cgaggatgac atccatgccc
      661 aggttcccgg tgcgacggcc atgatccaca ttgaggggcc cgagccggga tgggcccccc
      721 tcgccaccgg agacgagcca ccattctgcg tgcgggagtg cgacgcggcg acggacgacg
      781 agtgcccacg ggcgcggcgg ccctgagaac cggcccgctt agtgcctgaa tcccccgagg
      841 tgggcgatca ccatcgcgtc gttgatgacc gccacagcga cgtgggtgat gagggcggga
      901 agcatggatc ggccgcggta ggcgatgtaa ccgaggaaga ggcccgagac gagggtgccg
      961 tagagctcag actcgggctt cctcacatgg ccgaggacga atgggacgtt ctgaactact
     1021 acggccatga gccccagccg cgcctggagc gcaaactgca ggtacccgcg tagcgagaac
     1081 tcccagccga acatcagcag gacgacaaag cactggaaca cgacgaaggc accgaggctc
     1141 gcccgcgcgc ccctgaccat agggtagaaa gtctgtagtt ccctgaaagc gcccgtggcg
     1201 aaccacagaa tcggaatcat gatggccacg cccccgcaga cccaggggcc ccagcgccgc
     1261 cagtcgcccc agcagaggcc gtagtcggag agccggcgcc tccggatgag gaggatgacc
     1321 gcgaacggga tgaggacaag gaaggcggat gcgccggtca accagttgag gatcaagaca
     1381 tcgcgcgggc ctgtggggcg gagccacaag acctgcgagc cgatgctcgt gacgtggacg
     1441 agttgaccca ggtagcgacc cgagatgacg gccaccagca tgacctggag cgcctcgatc
     1501 tcggggctac gcagtggaat gtcgagtgcc tgggcgcagt acaggaccaa cagggcggca
     1561 ctggcgatgt cgaacgcgac accagccgcg acggggaggc ccatgaagaa ccgccggtag
     1621 atgtaagcca agcccgcagt gccgcccagc agcacgacgg catggatcca attcccacat
     1681 ggcggcttgg cggcgctgcc ccatggggtc tcagggcccg gcagtgcgtc ggcggtcggg
     1741 tggtcagata ccggtgttgc accggactcg aactcgggcg tcatcggatg gtcaacgccc
     1801 cacctcacgg ggctgcccct tgccggtgtt ctccccgtac tcgccggcgg cacccggccg
     1861 acgctcgacg cggcaaaggc ggatggcggc aagaggctca gcggaggcaa atgccgagcc
     1921 cgggcacacg gtgaccacca cggcctcgcg gtcggcaatg cgcacgagct ggcacacggg
     1981 gatgaaccgc tcggtcaccc aggcggtctg gcgggtgttg tcacccagga ggcgctgata
     2041 gaacgcggtc accacatcac tcggtttgtg gacgtagatc gtgtgtgcgt gagcatggta
     2101 gctcgcctcg ctgccgccgt ggccacgcgt gcccgacccg ccactgaccg cacgctcatc
     2161 ggggtaggcg agtgatcgaa gtgtccccag gtccgcgagc tccagcggag cgccgtccgg
     2221 cgactcccgg ggcaggacga tgagggattc gggtggctcg cagatccatg aggccaactc
     2281 ggccgcccag tcgacctggc tgttggcgcc caggaacttc agaacggccg gggatgcata
     2341 ggcctgcgcc gtggcgggaa tcgacgtgga cgggaggggc gccgggaagg ggctgtcgcg
     2401 cccggtagtc acgtcggccg gcgtcggcac cagcggccgc gccgtgttcg acccgaaacc
     2461 caagtcggct gcggccgggc tgtgccactg cccctgtgga atgggactgg cctggctctc
     2521 cgaacgcggc cgcagcacaa tggcgacggg agcagccaga accccaagca ccacgagggc
     2581 gcccaaccac gtccacggac ctcgcttgct gggcctcgac gccgcaggca agtcgacgag
     2641 catggagaag ccgcaccggg gacagtcgaa ggcccgcgga ggaagcggac ccccgcatcg
     2701 acggcattcg tggggcagtg gccactcctg tggcggacgc gccacgacgg cgcggcgggg
     2761 atctgcgaca ttctgcgcat ggggcagcgc agcatagcac caatgacacg t
//
LOCUS       DAOSPL010000513         2811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35766_length_2811_cov_3.015239, whole genome shotgun sequence.
ACCESSION   DAOSPL010000513 DAOSPL010000000
VERSION     DAOSPL010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35766_length_2811_cov_3.015239"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1879)
                     /locus_tag="PLQ54_10045"
     CDS             complement(<1..1879)
                     /locus_tag="PLQ54_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOS93643.1"
                     /translation="MARPTNQFPWEEVVRLAPRLDVAAFLDALRADPSYSGQIVHVRQ
                     FAAQAARMAEPRQPFHACALRFLEAQGVAGLYSHQAQALDLVRAGHNVVLATGTASGK
                     SLAYLIPIIETLSISPATASMLLFPTKALAQDQCRAFLGGLDAAELPAPVAGVYDGDT
                     PPDLRRKLRNSARVLFTNPDMLHAGILPQHARWGHFLQQLRWLVIDELHTYGGMFGSN
                     VANLMRRFFRVCARYGSSPQIIACSATMGNPRELAEHLTGEPFELVDDDGSPHGSRTF
                     VFWNPPQERHRRWRSRRSANVEAHELMARLVRQGVPTITFSKAKMTAEMIRRYVCERL
                     AADAPGLADKITTYRGGYLPEERREIERRLFAGELLGVSTTPALELGIDIGGLDASII
                     VGYPGTLSSFYQQAGRAGRRDRDSLVILVGLDTAVNQYVMAHPEFIFDRPVERAVVDP
                     DNPFILLGQLRCAAHESPIDSAELPGFGPHAGVVLRVLEGNSKLRRRGDRWYHASSEI
                     PQHEVSLRDCADRNILIQDIETQETIGELNQFDAPPIVHPEAIYMHRGDTYYVESLDM
                     VRNRALVRKVEVDYYTQPLGGTDVHHIDHILRERPFGAGRACWGEVTAHFQNVGYERI
                     RF"
     gene            2103..>2811
                     /locus_tag="PLQ54_10050"
     CDS             2103..>2811
                     /locus_tag="PLQ54_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93644.1"
                     /translation="MRVFGLVCGCAAVLATTSCAQQNAGPAGGDNHGGGNLFVGSPTI
                     EGVPIFPKDNPWNTDISGYPVHPNSEAYIASIGVDGHLHPDFGSFWEGGPMGIPFIVV
                     PGNQPRVPVAFEYADESDPGPYPVPPDAPIEGGADSEGDRHVLVLDRDNAKLYELFSA
                     YPEGDGWRAGSGAVFDLTRNDLRPEGWTSADAAGLPILPGLVRFDEVQAGEIRHALRF
                     TVQRTQRAYIHPATHWAS"
BASE COUNT          474 a          914 c          951 g          472 t
ORIGIN      
        1 agaaacggat ccgctcatag cccacgttct gaaagtgtgc cgtcacctca ccccagcaag
       61 ctcgcccggc gccaaatgga cgctcccgga ggatgtggtc gatgtggtgc acatccgtcc
      121 cgccgagcgg ctgcgtgtag tagtccacct cgaccttgcg cacgagcgcc cgattccgca
      181 ccatgtccag cgactccaca tagtacgtgt cgccgcggtg catgtagatg gcctcggggt
      241 ggacgatggg cggagcatcg aactggttca actcgccgat ggtctcctgg gtctcgatgt
      301 cctggatcag gatgttccgg tcggcgcagt cgcgcagcga aacctcgtgc tgtgggatct
      361 cgctggacgc gtggtaccac cgatcacctc tccggcggag cttgctgttc ccctccagaa
      421 cgcgcagcac aactcccgca tggggcccga accccgggag ctcggcacta tcgataggac
      481 tctcatgggc ggcacagcgg agctgcccca gcaggatgaa cgggttgtcg ggatcgacca
      541 cggccctctc gaccggccgg tcgaagatga actccgggtg agccatgaca tactggttca
      601 cggccgtatc gaggccgacg aggatcacca gtgagtcgcg atctcgtcgc ccggcccggc
      661 cggcctgttg gtagaacgac gacagcgtac ccgggtaacc gacaatgatc gacgcatcga
      721 ggcctccgat gtcgatgccc agctcgagcg ccggtgtagt actgacgccc aggagctctc
      781 cggcaaagag ccgccgctcg atctcacgac gctcctcggg tagatacccg ccgcggtaag
      841 tggtgatctt gtccgccaat ccgggggcgt ctgcggcgag tcgttcgcag acgtagcgcc
      901 ggatcatctc cgccgtcatc ttggctttcg agaaggtgat agtgggcacg ccttgtcgca
      961 caagtcgtgc catgagctcg tgggcctcga cgttggcact ccgccgcgag cgccaccgcc
     1021 gatggcgctc ctgcggcggg ttccagaaca cgaaggtccg gctgccgtgt ggactgccat
     1081 cgtcgtcgac gagctcgaat ggttcgccgg tgaggtgctc ggccagctcc cgcgggttcc
     1141 ccatggtggc cgagcatgcg atgatctggg gcgagctgcc atagcgggca cagacgcgga
     1201 agaagcgcct catcaggttc gcgacgttcg agccgaacat cccgccgtac gtatgcagct
     1261 catcgatcac gagccagcgc agttgctgca ggaaatggcc ccagcgcgcg tgctggggca
     1321 ggattcccgc gtggagcatg tcggggttgg tgaagagcac ccgggccgag ttgcgcagct
     1381 tgcggcgcag gtcgggaggc gtgtcgccat cgtagacgcc ggccaccggt gccggaagct
     1441 ccgcggcatc gagcccgccg aggaacgcgc ggcactgatc ctgggccagc gccttggtgg
     1501 ggaagagcag catcgaggcc gtcgccgggc tgatcgatag cgtctcgatg atcgggatca
     1561 gatacgccag ggacttcccg gaggccgtgc cggtggccag cacgacattg tgtccggcgc
     1621 ggaccaagtc gagtgcctgg gcctggtggc tgtagagccc cgccacgcct tgcgcttcga
     1681 ggaaacgcaa ggcgcaggcg tggaacggct ggcgtggctc agccatgcgc gcggcctggg
     1741 cagcaaactg gcgcacatgg acgatctgcc cgctgtagct gggatcggcc cgcagcgcat
     1801 cgagaaacgc cgccacatcc agccgtggag ccagccggac cacctcctcc caggggaact
     1861 ggttcgtggg acgagccaac gaccctccgg aacgagaccg cggggtcgca ctccattcgc
     1921 gccacgttga agatgagagc gtgttgcggg gggcccgacc cgaaacccgt tggtgggctt
     1981 ggtcggtctg gcggacgtgg cggtgctgga gcgtctgctc cgcggcagtt cgggagacgt
     2041 cggcggctcc atctgtggca cactgttgtt gagtgcccac ctcagacggc gagggatgcg
     2101 ggatgagagt gtttgggctt gtgtgcggtt gcgcggcagt actggccacg acgtcttgtg
     2161 cccagcagaa cgcgggcccg gcgggtggcg acaaccacgg tggtgggaac ctgttcgtgg
     2221 ggtcgcccac gattgagggc gtgcccatct ttccaaagga caacccgtgg aacaccgaca
     2281 tctcgggcta tccggtacac ccgaactccg aggcctacat cgcgtccatc ggtgttgacg
     2341 ggcacctgca cccggacttc ggctccttct gggaaggcgg gcccatgggc atccccttca
     2401 tagtggtgcc gggaaaccaa cccagagttc ccgtggcgtt cgagtacgcc gacgagagcg
     2461 atcccggtcc ataccccgtc ccgcctgatg cgcccatcga gggcggcgct gactccgagg
     2521 gcgaccggca cgtcctcgtg ctcgacaggg acaacgccaa gctctatgag ctcttctccg
     2581 cctaccccga gggagacggc tggcgtgccg gatccggcgc cgtcttcgac ctgacccgca
     2641 atgatctgcg gcccgagggc tggacgtcag ccgacgcagc tgggctgccg atcctgcccg
     2701 gcctggtccg gttcgacgag gtacaggccg gcgagatccg gcacgctctg cggttcaccg
     2761 tccagcgcac tcagcgggcc tacattcatc cggcgacaca ctgggccagc c
//
LOCUS       DAOSPL010000514         2811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35769_length_2811_cov_2.595428, whole genome shotgun sequence.
ACCESSION   DAOSPL010000514 DAOSPL010000000
VERSION     DAOSPL010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35769_length_2811_cov_2.595428"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PLQ54_10055"
     CDS             complement(<1..256)
                     /locus_tag="PLQ54_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93645.1"
                     /translation="MDRLGTRGLVGLVLASLIALAAVPTAQAQQSSAPVALRLSGTEG
                     ARAQYSWTTEGRVSFQIPEDLVKNVPFDTRSPLKVKATSLL"
     gene            complement(305..1033)
                     /locus_tag="PLQ54_10060"
     CDS             complement(305..1033)
                     /locus_tag="PLQ54_10060"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOS93646.1"
                     /translation="MKAVILSAGYGTRLRPITDYLCKALVPVNGVPVLERVIRRLAGY
                     GFNEFVVAVSHLGEQCVHYFGNGERLGVTLRFSITDEPQGTAGEIAAARHWLEGEPDF
                     LVYYGDILSNFDVAGLVTRHQQKDPLATIGLVRSYRVATGIAEVNADGRVVAFVEKPP
                     LPQPTNAAIMMFSGRALPWFRKGEDISIDVIPAMLRADEWVEGFIDENAYWHDVGQPA
                     SLARAIEAVAAEEGDARTIILGRA"
     gene            complement(1384..2709)
                     /locus_tag="PLQ54_10065"
     CDS             complement(1384..2709)
                     /locus_tag="PLQ54_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOS93647.1"
                     /translation="MNVIVIVADSFRTDCLGCYGGEGIDTPRLDELGAESILFTQAYA
                     DGLPTIPARHSMLTGRYTFPFRGWQPLEHDDVTLPEALWDKGYDTALITDVYHMLKPG
                     MNFARGFAYTRFIRGQEYDPYIVDPSIEVDVDSFYKADGLNDEIFKPRFAQYLKNVSR
                     RQAEEDYFAAQVMQAGMDWLETRHADQPFLLWLDCFDPHEPWDPPPPFDTMYDPDYTG
                     KKILDPVPGNVQGYLSPEELAHVRALYAGEVTFVDKWVGLLLDRARSLGLLEKSLVVF
                     TSDHGVPFGEHGIIRRCRPWLHQELVNVPLMLRYPDGTGAGRICEAFAQSCDLMPTIL
                     GFLGIEGPAGMTGADLGPLVRGEAQSVRDYAYSGFHMRAWSIRSQEHSYLLSLEGEPS
                     ELYNRAEDPDEFTNIYEGSPDVAEELEGELRRFVESLVPEPDAEPGAPE"
BASE COUNT          476 a          931 c          904 g          500 t
ORIGIN      
        1 acagcaggct tgtcgccttg accttgagag ggctcctggt gtcgaaaggg acgttcttga
       61 cgaggtcctc ggggatctgg aacgagacgc gcccctcggt ggtccagcta tactgagcgc
      121 gggccccttc cgtgcccgac aagcgcagcg cgacgggggc agacgactgt tgcgcctgag
      181 ccgtcggcac ggccgccagc gcaatcagtg acgcgaggac cagccccacc agtccacgtg
      241 tgcccaagcg atccatgggg atctcctacc cttcctgcag tgcactgaga cgcgagcggg
      301 gacgttaggc ccgccccagg atgatggtgc gcgcgtcgcc ttcttcggcc gcgacggctt
      361 cgatggcccg ggccagcgag gccggttgac cgacgtcatg ccagtacgcg ttctcgtcga
      421 tgaacccctc gacccactcg tcggcgcgaa gcatagctgg gatcacgtcg atcgagatgt
      481 cctcaccttt tcggaaccag ggcagagccc gaccggagaa catcatgatg gcggcattgg
      541 tcggctgagg caggggcggc ttttcgacga aggcgacgac ccggccgtcg gcgttgacct
      601 cggcaatgcc ggtggcgact cgatagctcc tcaccagccc gattgtcgcc agcggatctt
      661 tctgctgatg cctggtcacc aagccggcca catcgaagtt gctcaggatg tcgccgtagt
      721 acacgaggaa gtccggctcc ccctcgagcc agtggcgcgc tgccgcgatc tcgcccgcgg
      781 tgccctgtgg ttcgtcggtg atcgagaagc gtagggtgac ccccaagcgt tccccgttgc
      841 cgaagtagtg gacacactgt tcgcccagat gagacacggc caccacgaac tcgttgaagc
      901 cgtagcccgc caggcggcga atcacgcgct ccagcaccgg caccccattc acgggcacca
      961 gtgccttgca caggtagtcc gtgatgggcc gcagccgggt accgtatccc gcgctgagga
     1021 tgaccgcttt cacgccatgg cctcatttcc gttggggctg ccgcgtggga tcaccgcgtg
     1081 ccaacgccgt gccagatcgg tccgacgccc ctgtcggacc agtatagtcc atcggaccca
     1141 cccctgtgtg gggggcgagc tcccgggggc cttccgttct ccagttcggg acggatggca
     1201 cccctcccgc cttgcgggcc cttcccattc gtcccatcgc tgatggttgg gtggatctcc
     1261 ggaagtgccg catggcggga accaaccgca ggcgggcggg accccatgcg gcccgaggcc
     1321 accgacgcct caggcggctc gtgaccgggt ggaagtgggc acaggtcccg cggcgcaccc
     1381 ggcctattcc ggcgcgcccg gttcggcgtc cggttccggc accaggctct cgacgaaacg
     1441 ccggagctct ccctcgagct cctcggcgac atcagggctg ccctcgtaga tgttggtgaa
     1501 ctcgtcgggg tcctcggccc ggttgtacag ctctgagggt tcgccttcca gcgagagcag
     1561 gtagctgtgc tcctgggagc ggatgctcca ggcgcgcatg tggaagccag agtaggcgta
     1621 atcgcggacc gactgggcct cgccccggac caacggtccc aggtcagccc ccgtcatgcc
     1681 cgccggtccc tcgatgccga ggaagcccag gatcgtgggc atcaagtcac agctctgggc
     1741 aaaggcctca cagatccgcc ccgcgcccgt accatccggg taccgaagca tgagaggtac
     1801 gttcacgagt tcctggtgca accaagggcg gcagcggcgg atgatgccgt gctcgccaaa
     1861 cggtacgcca tggtcactgg tgaagacaac caacgacttc tccagcagac cgagtgaccg
     1921 ggcacgatcg agcaagagcc ctacccactt gtccacgaag gtgacttcgc cggcatagag
     1981 cgcccggaca tgggcaagct cctcggggga cagatagccc tggacattgc cgggcaccgg
     2041 gtccaggatc ttcttcccgg tgtagtccgg gtcgtacatg gtgtcgaacg gcggtggcgg
     2101 gtcccatggc tcgtgcgggt cgaagcagtc cagccacagc aggaatggct ggtcggcgtg
     2161 gcgagtctcg agccagtcca tccctgcctg catcacctgg gcggcgaagt agtcctcctc
     2221 ggcctgccgt ctgctgacgt tcttgaggta ttgagcgaaa cgaggtttga agatctcatc
     2281 attcagtccg tcggctttat agaatgagtc cacgtcaacc tcaatgctgg ggtcgacgat
     2341 gtaggggtca tactcctgcc cccggatgaa ccgggtgtag gcaaacccac gcgcgaagtt
     2401 cattccgggt ttgagcatgt gatagacatc ggtgataagt gctgtgtcgt atcctttgtc
     2461 ccagagcgcc tcgggcaggg tgacatcatc atgctccagc ggctgccatc cgcggaatgg
     2521 gaacgtgtag cgccccgtca acatgctgtg gcgcgcgggt atggtcggca gcccgtcggc
     2581 gtaggcctga gtgaacagga tgctctccgc gcccagctca tcgagccgag gcgtgtcgat
     2641 gccctcgccg ccgtagcacc ccaggcagtc ggtccggaag ctatccgcca cgatcacgat
     2701 gacgttcatc gtctacctcc tgccgccgcg tgcacggcgg ctttgtccgc tctatggcct
     2761 tcggcgcacc gccgcgcagg cccttcacgt aacgccccga ctggggtcgc c
//
LOCUS       DAOSPL010000515         2808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35824_length_2808_cov_2.673810, whole genome shotgun sequence.
ACCESSION   DAOSPL010000515 DAOSPL010000000
VERSION     DAOSPL010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35824_length_2808_cov_2.673810"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="PLQ54_10070"
     CDS             <1..1205
                     /locus_tag="PLQ54_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93648.1"
                     /translation="ISGDGQAFGGFDVSRRRVLGRPGPGGGWQTVAQTDYTGRNLENQ
                     GEVMVDLTPFLGSGQALVRFEDILPGDGWGPYITRLVAEGVRDGKPVALDVVPGSAEE
                     RDLVVDDRGSIVPDLGARFCDAQTSVTYGFAFDAGSSATLRVTVGNQYRITAAAGKRQ
                     DAREFAAQGEAQPLGAARIAVPMAQAVVDYPGSGAAPVYANDESVLLSEQRVGQGRVL
                     FCGLAPSYFAASAEAAGQMRALARYAYGPVGDWREPHHIALRRGRYVMVKTFDEPLDL
                     EGRFIPLLRADLPVVAGCKLGPDQVALLYDAAPDLAGNPRLIYSSSCVEWRSDEPERV
                     RAIVSGAEGTFGVCRFSTGGRPVGTLAAQDAKGRPVDVERVVEGDTLLLRYPNHPFGL
                     ALSVEWGR"
     gene            complement(1219..2436)
                     /gene="amrB"
                     /locus_tag="PLQ54_10075"
     CDS             complement(1219..2436)
                     /gene="amrB"
                     /locus_tag="PLQ54_10075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOS93649.1"
                     /translation="MDTHPRRRALEVIPLEAKGQRAVALRDPADHSRQPVILTAAAFL
                     LWSMCDGEHTVRDLQVELTRRVGELVMSSTIEAMLREFDDNLLMDTPTYHDYVRGRLE
                     AYRQRASRPAIHAGGGYPADPKELARLLDGLLDSAPPFPGLPADVMGLIAPHIDLARG
                     GPVYGSTYAALRQSVPEARRFVVLGIAHHATQTPLVLTRVPYETPLGRCTLDAAFIDS
                     LALRCSQDLFADELAHESEHSIEFTALFLNHIYGPDVTLVPILCGSFEPGGGEGESPA
                     DLGPVRECVEALAAGVAEADGPTCVIAAVDFSHIGPQFGDPRPATPAFVEWAEARDQQ
                     LLNLAAAGDAEGFYAAAVANDNESHVCGVPAIYMLLAALAPVTGVLIDHAHAVTDETR
                     SAVTFAGMVLAKA"
BASE COUNT          416 a          917 c         1049 g          426 t
ORIGIN      
        1 tcatttctgg ggacggccag gccttcggtg gattcgatgt gagccgacgg cgcgtgttgg
       61 gccgtccggg cccaggcggg ggctggcaga ccgtggcgca gaccgactac acggggcgca
      121 accttgagaa ccagggagag gtcatggtcg acctgacgcc cttcctgggc tcagggcagg
      181 cactggtacg cttcgaggac atcttgcccg gcgacggctg gggtccgtac atcacgcggc
      241 tggtcgctga aggggtgcgc gacggcaagc cagtggccct cgacgtcgtc cccggcagtg
      301 ccgaggagcg cgatctggtc gtggatgacc gtggtagcat cgtcccggac cttggcgctc
      361 gtttctgcga cgcgcagaca tcggtgacgt acggattcgc gttcgacgcc ggctcgagcg
      421 cgacgctccg ggtgaccgtc ggcaatcagt accgcatcac cgcggcggcg ggcaagcggc
      481 aggacgctcg cgagtttgcg gcgcagggcg aggcccaacc gttgggcgcg gcgaggatcg
      541 ccgtgcccat ggcacaggcg gtggtcgact acccgggctc gggcgccgct ccggtgtacg
      601 cgaacgacga gagcgtgctg ctatcggagc agagagtcgg gcaaggccgg gtgttgttct
      661 gcggcctggc gccctcatac ttcgcggcgt cggcggaggc cgccgggcag atgcgcgcgc
      721 tagcacggta tgcgtatggc cccgtcggcg actggcgcga accgcatcac atcgccctgc
      781 gccgtgggcg gtatgtcatg gtcaagacgt tcgatgagcc cctggatctc gaggggcgct
      841 tcatccctct gctccgggcc gatctgccgg tcgtcgccgg ctgcaagctc ggccccgacc
      901 aggtcgccct gctctacgat gcggcgcccg acctggcagg gaatccgaga ctcatctact
      961 cctcgtcgtg cgtcgagtgg cgcagcgatg agccggagcg cgtccgtgcc atcgtctcgg
     1021 gcgctgaggg aacgttcggt gtctgtcgct tctccaccgg agggcgaccc gtcggcacgc
     1081 tggcggccca ggatgccaag ggccggcccg tcgacgtgga gcgggtggtc gagggcgaca
     1141 cgctcctgct ccgctacccg aaccacccct tcggcctggc gctgagcgtg gagtggggaa
     1201 ggtagcagcg cgggctcctc aggccttcgc caagaccatg cccgcgaagg tgacggctga
     1261 ccgcgtctcg tcggtgacgg cgtgggcgtg atcaatgagc actccggtta cgggtgcgag
     1321 ggcggcgagg agcatgtaga tcgccgggac gccgcacacg tggctctcgt tgtcgttggc
     1381 gacagccgcc gcgtagaacc cttccgcgtc gccggcggcg gcaaggttca ggagctgctg
     1441 gtcgcgcgcc tcggcccact cgacgaaggc cggcgtcgcc gggcgcggat cgccaaactg
     1501 aggcccgatg tggctgaagt ccaccgcggc aatgacgcac gtgggcccat cggcctcggc
     1561 cacccctgca gccagcgcct cgacgcattc gcggaccggg cccaggtcgg ccggggactc
     1621 cccctctccg cccccaggct caaaggaacc gcacagaatc ggcacgaggg tcacatcggg
     1681 gccgtagatg tggttcagga acaacgcggt gaactcgatg gagtgctcgg actcgtgcgc
     1741 cagctcatcg gcgaacaggt cctgcgagca ccggagggcc agggagtcga tgaacgcggc
     1801 atccagcgtg catcggccga ggggcgtctc gtagggcacg cgggtgagca ccagcggcgt
     1861 ctgggtcgcg tgatgcgcga tcccgagcac cacgaagcgc cgggcctccg ggacggactg
     1921 gcgcagggct gcgtaggtcg agccatacac ggggccgcca cgcgccaagt cgatgtgggg
     1981 tgcgatcagg cccatgacgt cggccggcag tcccgggaag ggaggcgccg aatcgaggag
     2041 gccgtcgagg agccgagcga gctccttcgg gtcggccgga tagccccctc ccgcgtggat
     2101 ggccggacgg ctggcgcgct gacgataggc ctcgaggcgc ccgcgaacat agtcgtggta
     2161 ggtgggggtg tccatcagga gattatcgtc gaactcgcgg agcatcgctt cgatggtcga
     2221 gctcatcacg agctccccaa cgcgccgcgt gagctcaacc tgcaggtcgc ggacggtgtg
     2281 ctcgccatcg cacatcgacc acagtaggaa tgcggccgct gtcaggatga ctggctgccg
     2341 cgagtgatcg gcgggatccc gcaaggcgac ggcacgctgg cctttggcct ccagcgggat
     2401 cacctcgagt gcccggcgcc gcgggtgagt gtccatcgat gcccctccaa ggcattggcg
     2461 gtgactgggg ggccccagcc gcccctcggc ctggtggcgc aggggcgcca tgggggaggc
     2521 gcgcaccggg cacatgggta gcactctggc gccgccacaa ggcggttgtc ccttccgcga
     2581 cgagggggta cgctccttgc tgtccgtccg gaggcgagcg ctctgcaggg ccgcgacgcc
     2641 agcgctagtc ggtgtcttcg ggatgaggac agaccgcagg gactcgcgcg aacgaggggg
     2701 gtgacgtatg gcggtcaggc tctggctggt gtgcggcatg gctctggccg gcgccgcgct
     2761 ggccgccatg cggtacgccg cccccgctcc gccgccgccg gaggtgtc
//
LOCUS       DAOSPL010000516         2805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35861_length_2805_cov_3.719636, whole genome shotgun sequence.
ACCESSION   DAOSPL010000516 DAOSPL010000000
VERSION     DAOSPL010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35861_length_2805_cov_3.719636"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PLQ54_10080"
     CDS             <1..464
                     /locus_tag="PLQ54_10080"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOS93650.1"
                     /translation="IGIFERGRLIQVGTPDEVFARPSSEFVAHFTGARNVIDGEVARQ
                     DGAFVFRGGGISVPVPATRQGPAKLVVRPEELRIVTEPTSEGPTLQGTVSRISDRGAS
                     VRLGVTVAAGEWSLLLAKREARALGLAVSQPVTFELPTQALHLIWLADPPG"
     gene            624..>2805
                     /locus_tag="PLQ54_10085"
     CDS             624..>2805
                     /locus_tag="PLQ54_10085"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HOS93651.1"
                     /translation="MATTSLILGPIGLMALVLAPADSAAQFIDTWLVAGPFANDAANT
                     AFDTDHVGVAAVWPQPGDAWRVFDDRVFSRNYDDYQDLHSYLTVVRGLSAGPAVMYAH
                     TYVFSESAQSGELRMGADNEHKVFVNGSLVGASTDSHPWRDSVAYPIGLDRGWNRLMI
                     QVGNQEETRLGFYARLCAADGSALPGLTYALAVDGPLAVAHAALPAGFREWPYVALDP
                     MADPDPDMGAYLTRKPHHTAHASPFRLAALGGQPPYAWSMSGGDLPSGLRLADDGVIS
                     GTVARDAPLGEYRLRAAVTDAAGSRAEAELTLPVRERPNKWYEDARLVALMHNPESIL
                     QATDDDLDQFADLMKRQGYALGMMISYNNGDFAYRWPSSFEPDNPRGDLAGRYKAALE
                     AHGVKFGMYMGNLIGPNHGGDNGAIRMVEEAVVRYKPAALWFDWASPDPDGYVSLDAL
                     YSMIRAQSPETVIVLNGVQTLYQGDWDVVCVEGWGAWGDKIWATWPFELKWPKRVPVE
                     TWRMLADPGFEYSRDIAPDWQEYLRVQLTIIGQGFVANMDHSPTIATPYRRLAESVVA
                     QAHMAMADWASPPGIPPLYESYTNVSPGPIRDADWGYTTVSLGREALYIHMLENPFGK
                     TGMPAEGTIALPCPKATEVRWMNRDVSIPFRQDGGQLVIPLGDVEADPIDTILKITLA
                     APLNDEQVAPAPDLRPIPPGNLATHKPAKLLSTDGTHSLIASGFA"
BASE COUNT          448 a          968 c          917 g          472 t
ORIGIN      
        1 gcatcggcat cttcgagcgg ggacggctga tacaggtggg tacgcccgat gaggttttcg
       61 cgcgcccgtc ctcggagttc gtcgcccact tcacgggggc tcgtaacgtg atcgacggtg
      121 aggtggcgcg ccaggacggc gccttcgtgt tccgcggcgg cgggattagc gtgccggtgc
      181 ctgccacgcg ccaggggccc gccaagctcg tggtgcgccc ggaggagctc cggatcgtga
      241 cggagcccac ttcggagggg ccgacgctcc agggcaccgt ctcgcggatc tccgaccgcg
      301 gcgcttccgt ccgtctcggc gtcactgtcg cggccggcga gtggtctctc ctcctggcca
      361 agcgcgaagc cagagcactt ggcctcgcgg tgagccaacc ggtgactttc gagctcccga
      421 cgcaagcact acacctcatc tggcttgcgg acccacccgg ctagcaccgc gcaccttcgt
      481 gcagccgaat ggcgggccgc gccatgggca tgggcggcgg tcacgacgtg cgtcttccgg
      541 ccgagtgaca ccgcgttgct acaggagaac ccccgtgcga tggtgaagcc tcctacacga
      601 tcccaccacc catggaggct ctcgtggcga cgacttcgct gatccttggc cccatcggcc
      661 tgatggctct cgtcttggcg ccagccgact cagccgcgca gttcatcgac acgtggctcg
      721 tggccgggcc attcgccaac gatgccgcca acacggcctt cgacaccgac cacgttggcg
      781 tggctgccgt gtggccgcag ccgggcgacg cgtggcgcgt gttcgacgac cgcgtcttct
      841 cgcgcaacta cgatgattac caggacctgc acagctacct gaccgtcgtt cgcggcctgt
      901 cggctggccc ggccgtcatg tatgcgcaca cctacgtgtt cagcgagtcg gcccagtcgg
      961 gcgagttgcg gatgggtgcc gacaacgagc acaaggtctt cgtcaacggc agcctggttg
     1021 gcgcgagcac cgatagtcac ccctggcgcg actcggttgc ttacccgatc gggctggatc
     1081 ggggctggaa ccgcctgatg atccaggttg gcaaccaaga ggagacccga ctcgggttct
     1141 acgctcgcct gtgcgctgcg gacggaagcg ctctgcccgg cctgacctac gctctggctg
     1201 tcgatgggcc gctggcggta gcgcacgccg cgcttcccgc aggtttccgc gagtggccct
     1261 atgtggccct cgatccgatg gccgatcccg atccagacat gggcgcctac ctgacccgca
     1321 agccacacca cactgcccac gcgtcaccgt tcaggctggc cgccctgggc ggccagccgc
     1381 cgtacgcgtg gtcgatgtct ggtggggacc taccgtcggg gctcaggttg gctgatgacg
     1441 gggtcatctc gggcaccgtc gcgagagatg ccccgctcgg ggagtaccgt ctccgcgccg
     1501 ccgtcaccga cgcagccgga tcgcgcgccg aggccgaact gacgcttccg gtccgcgaga
     1561 ggccgaacaa gtggtacgag gacgcgcggc ttgtggcgct gatgcacaat cctgagagta
     1621 tcctgcaggc gaccgatgac gacctcgacc agttcgccga cctgatgaag cgtcagggct
     1681 atgcgctggg gatgatgatc tcctacaaca atggcgactt cgcctatcgc tggccctcga
     1741 gcttcgagcc ggacaacccc cgcggcgacc tggcggggcg ctacaaggcc gcgctcgagg
     1801 cccacggcgt gaagttcggc atgtacatgg gcaatctcat cgggccgaac cacggcggcg
     1861 acaacggagc gatccgcatg gtcgaggagg ccgtggtccg ctacaagccc gcggccctgt
     1921 ggtttgactg ggcgagcccc gaccccgacg gttacgtctc actggatgcc ctctacagca
     1981 tgatccgggc gcagagtccc gagacggtca tcgtcctcaa cggcgtgcag acgctctacc
     2041 agggcgactg ggatgtggtc tgcgtcgagg gctggggcgc gtggggcgac aagatctggg
     2101 ccacctggcc cttcgagctg aagtggccca agcgcgttcc cgtggagact tggcggatgc
     2161 tcgccgaccc tgggtttgag tattccaggg atatcgcccc tgattggcag gagtacctgc
     2221 gcgtccagct caccatcatc ggccagggct tcgtggccaa catggaccac agcccgacca
     2281 tcgccacacc gtaccgccgg cttgccgagt ccgtcgttgc ccaagcgcac atggctatgg
     2341 ccgactgggc cagcccgcct ggcattccgc cactctacga gtcctacacc aacgtgagcc
     2401 ctggtccgat ccgcgatgcc gactggggct acaccaccgt cagcctcggg cgggaagcgc
     2461 tctacatcca catgctcgag aacccgttcg ggaagacggg gatgcccgcg gaggggacga
     2521 tcgcgctccc ctgtcccaag gccaccgaag tccggtggat gaaccgggac gtgtccatcc
     2581 cgttccgcca ggatggtggt cagcttgtca taccactcgg tgatgtggag gccgatccca
     2641 tcgacacgat tctcaagatt accctcgcgg ccccgctgaa cgatgagcag gtggcgcccg
     2701 cccccgatct gcggccgatc ccgccgggca acctggccac gcacaagccg gcgaagctgc
     2761 tgagcaccga tggcacgcac tcgctgatcg cgtcaggctt cgcct
//
LOCUS       DAOSPL010000517         2805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35871_length_2805_cov_2.520364, whole genome shotgun sequence.
ACCESSION   DAOSPL010000517 DAOSPL010000000
VERSION     DAOSPL010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35871_length_2805_cov_2.520364"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..>2805
                     /locus_tag="PLQ54_10090"
     CDS             30..>2805
                     /locus_tag="PLQ54_10090"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93652.1"
                     /translation="MDGHPSFGPCAVALNGVPAGPSDAVAGLRVAAVPGGPQMALMAR
                     PATAVVALARGEEARLRVPVGGLAGPVTVTAEAAGDPGASAAISLADGPVRWQEVKPG
                     ATVVRAEAHVTGDVVVSLASKATRDGSTVRWSSIRLIAREREFEIPLVAESPIEDLGP
                     PPELPALRPAIEQSLIEWDWACQDGIGTARCPSTYEEAIRRTIARGDALLADLRSRAA
                     GMGDVARRWEAVRARLSELDGADPMGVKARKLWLEVHAARRQMALSNPLAHVGPLLFV
                     KQVPAAFSHQLTQYYGRYARPGGGVFVLDQPGRSMACRRLAAGALPEGSYQHPELSYD
                     GQRVLFSFCATDRGPGDSFVGNPGRYYHLYEMGIDGSDLRQLTDGAFDDFAPRYLPDG
                     RLVFISTRRLGWHRCGTPGCENYTLAVAEADGSNARPVSYHETQEWDPAVLDDGRIIY
                     TRWDYVDRHAVFYEQLWATLPDGSLPSTFYGNNTFNPVGVWEPRPVPGSPRIMATAAP
                     HHGMTAGSIVLVDVGYGVDGEAPITRLTPDALFPESESTLAASWRADIPGLSVDVPIE
                     EQRWPGHCYRSPYPLSEDYFLAAYSYDRLIGEPSPNPRNMFGLYLVDRWGNKELLYRD
                     PNIASLWPVPIRPRTRPPVVVSGATGNGKGTGTILIQDIYESAPAIPRDVVRKLRVVQ
                     LVPKSTPGANNPPVGLANASPGKQVLGTVPVEPDGSAWFEAPAGVPLSFQALDERGMA
                     VQVMRSDTYLQPGQSMSCVGCHEPRTTAPPNRSLRALSRAPSKIAPGPDGSKPLSYPL
                     LVQPVLDKHCVSCHSGGQPAGGVSLTAQPEGRYTVSYNALAPRVSISQWGVGDFRRAN
                     SEPLATPLFFGAHGSPLARLLLSGHQGVKLEGEDWDRLVTWMDANALFYGTFNFEDQA
                     RQQRGERIEGP"
BASE COUNT          400 a          973 c          993 g          439 t
ORIGIN      
        1 cgtgcggcct ggctgaggtg gccgtgcgca tggacggcca cccttccttc ggcccgtgcg
       61 cggtggcgct gaatggcgtg ccggcggggc cgagcgatgc cgtcgccggc ctgcgggttg
      121 cggccgtgcc gggaggaccc cagatggcgc taatggctcg cccggccacc gccgtggtcg
      181 cactggcgcg gggcgaggaa gctcggctac gggtgccggt cggtggtttg gccgggccgg
      241 tgacggtgac ggccgaagcg gccggagacc cgggcgcctc ggcggccata tcgctggccg
      301 atgggccggt ccgatggcag gaggtcaagc cgggtgccac ggtggtccgc gccgaggccc
      361 atgtgacggg cgacgtggtg gtctccctcg catcaaaggc gacacgcgat ggctcgacgg
      421 ttcgatggtc ctccatccgt ctgatcgcgc gggagcgcga gttcgagatc ccactggtcg
      481 ccgagtcgcc gatcgaggac ctgggaccgc ccccggagct ccccgcctta cgtcccgcca
      541 tcgagcagtc cctcatcgag tgggactggg cgtgccagga cggaatcggc accgctcggt
      601 gcccgagcac gtatgaggag gccattcggc ggaccatcgc ccgtggagac gccctgctcg
      661 cggatctgag gtcgcgggca gccggcatgg gcgacgtggc gcgccgctgg gaggccgtgc
      721 gggctcggct ctccgagctc gacggagccg atccgatggg cgtgaaggct cgaaagctct
      781 ggctcgaggt tcacgccgcg cggcggcaga tggcgctcag caaccccttg gcccacgtgg
      841 gtcccctgct gttcgtcaag caggtccccg ctgcgttcag ccaccagctg acccagtact
      901 atggacgcta tgcccgcccc gggggcggcg tgttcgtgct cgaccagccg ggccggtcca
      961 tggcatgccg gcggctcgcg gccggggcgt tgcccgaggg cagctatcag cacccggagc
     1021 tcagctacga tggccagcgg gtgctcttct cgttctgcgc gaccgaccgt gggccgggcg
     1081 actcgttcgt ggggaatccg gggcggtact accacctcta cgagatgggc atcgatgggt
     1141 ccgacttgag gcagctgacc gatggcgcct tcgacgactt cgcaccgcgg tacctgcccg
     1201 atggccgtct ggtgttcatc tcaacccggc gtctgggatg gcaccggtgc ggaactccgg
     1261 gctgcgagaa ctacacgctc gccgtggccg aggccgacgg gtcgaatgcc cggccggtct
     1321 cataccacga gacccaggag tgggatccgg cggtgctcga cgatggccgg atcatctaca
     1381 cccgatggga ctacgtggac cggcatgcgg tgttctacga gcagctctgg gccaccctcc
     1441 ccgacgggag cctgccgtcg accttctacg ggaacaacac gttcaaccct gtgggcgtct
     1501 gggagccccg cccggtgccc ggctcgcctc gcatcatggc caccgcggcg ccgcatcacg
     1561 gcatgaccgc cggctccatc gtcctggtgg atgtcggcta cggcgtcgac ggcgaggccc
     1621 cgatcactcg gctcactccc gatgcgctgt tccccgagag cgagagcacg ctggcggcgt
     1681 cgtggcgcgc cgacatcccc ggcctctcgg tcgacgtgcc catcgaggag cagcggtggc
     1741 cgggccactg ctaccggagc ccttaccctc tgtcggagga ctacttcctg gccgcgtaca
     1801 gctacgatcg gctgatcggt gagccctcgc ccaatccgag gaacatgttc ggcctgtacc
     1861 tggtggatcg ttgggggaac aaggaactgc tgtaccgcga ccccaacatc gccagcctct
     1921 ggcccgtgcc gatccgcccc cggacgcgcc cgcccgtggt ggtctcgggc gccacgggga
     1981 atggcaaggg cacgggcacg atcctgattc aggacatcta cgagagcgct cccgcgatcc
     2041 cccgcgatgt ggtgcgcaag ttgcgcgtgg tccagctggt tcccaagtcg accccgggag
     2101 ccaacaaccc gccggtgggc ctggccaacg cctcgcccgg gaagcaagtg ctgggcacgg
     2161 ttccggtcga acccgacggg tcggcctggt tcgaagcccc ggccggggtg ccgctgtcgt
     2221 tccaggcact ggatgagcgg ggcatggctg tccaggtcat gcggagcgac acctatctgc
     2281 agccgggcca gtccatgtcc tgcgtggggt gccatgaacc gcggacgacg gctccgccca
     2341 accgctcgct tcgcgccctg tcgcgagcgc cctcgaagat cgctccggga ccggatggct
     2401 ccaagccgct gagctaccct ctcctggtgc aacccgtgct cgacaagcac tgcgtctcgt
     2461 gccactcggg cggccaaccc gcgggtggcg tctcgctgac cgcgcaaccg gagggccgct
     2521 acaccgtctc gtacaacgcg ctggcgcccc gagtgtcgat ctcgcagtgg ggcgtcggtg
     2581 acttccgccg ggccaacagc gagcccctgg caaccccgct gttctttggg gcccacggga
     2641 gcccgctggc ccgcctgctg ctgagcggac accagggagt caagctcgag ggcgaggact
     2701 gggaccggtt ggtgacctgg atggatgcca acgccctgtt ctacggcacc ttcaacttcg
     2761 aggaccaggc gcggcagcag cgcggagagc ggatcgaggg gcccg
//
LOCUS       DAOSPL010000518         2804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35884_length_2804_cov_3.632230, whole genome shotgun sequence.
ACCESSION   DAOSPL010000518 DAOSPL010000000
VERSION     DAOSPL010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35884_length_2804_cov_3.632230"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLQ54_10095"
     CDS             complement(<1..392)
                     /locus_tag="PLQ54_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93653.1"
                     /translation="MVQRCGMSQTNRKSLSPSEAQELERLRRENEELKQLNEQLVREL
                     GYVNLEKLAWESEVQRRGLRPPKGGASLKEAVDAVLSLWRPAFGQERVFERARDLAYG
                     TLLNLGRHTVTGALCALGHDQRDWTADYR"
     gene            complement(613..1044)
                     /locus_tag="PLQ54_10100"
     CDS             complement(613..1044)
                     /locus_tag="PLQ54_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93654.1"
                     /translation="MTDAPTRHPSPPALWFFLGVLVTLVLLAITGLVLLRAGGVETIV
                     GSAIRSAVHDVANDKAVRQSLSDAVGSALTDDAVQDRVRQAVSEVADEVAQSDAMREH
                     LRMALRETLQDGAVRRSLRAAVREALVEVTNEALRGQRAAE"
     gene            complement(1041..>2804)
                     /locus_tag="PLQ54_10105"
     CDS             complement(1041..>2804)
                     /locus_tag="PLQ54_10105"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93655.1"
                     /translation="IRRMSANKDGDYLPHALDNGLLTYTRWEYQERNWAHIQSVWVMN
                     PDGTGADALYKQHFNDPWAIEEAHSIPGSNKLLAIATGHHTLPVGPVVLVDPKMGIND
                     PRGIGIITPGTSPPEGGMAGRPVPEGGVPGGGGLYATPWPLSEKACLASFTYSDVMTD
                     EKGYGLYLLDVYGTRELVYRDPDISCFYPIPLRPRTRPPIIASVWDPGARAASCLVTD
                     VTMGMDGIEPGRVKYLRIAEGVAWPYDLALGGLRYEPDVKPVMINWNPVRVLGTVPVE
                     LDGSAHFEVPPDTPVYFQALDERMMELRRMRSFISFQPGERRSCSGCHETRGTAPPNT
                     AVPMAAQRAPSPLVPPPWGDGVISFLRDVQPVFDRHCVVCHSGLQPAGGHDFSGGLTP
                     TANTAWDTITRARLVARSNVGDDARITPPLEFGSNKSRIVEVIETTHRGRVRLSEDDR
                     IRLVTWIDANAPYHSGFINKRPERPVYDLAADAALRARLVDVSRRRCGACHEPEQVAR
                     LDWIDLRDPARSRFLAAPLATESGGWGRCQPAAYARADDPDYAATLSAVRHAVVRAWA
                     APRRDLATLDPPIDRQVADAP"
BASE COUNT          432 a          981 c          934 g          457 t
ORIGIN      
        1 cggtagtctg ccgtccagtc gcgttggtca tgccccaacg cgcacagggc gcccgtcacc
       61 gtgtgccgac ccaggttcag aagcgtgccg tatgccaggt cccgcgcccg ctcgaacacc
      121 cgctcttgcc cgaaagccgg ccgccacagg ctcaacaccg cgtccaccgc ctctttcagg
      181 ctggcgcccc ccttcggcgg acgcaaccct cgacgctgga cctcactctc ccacgccagc
      241 ttctccaggt tcacataccc gagctcacgc accaactgct cattgagctg cttgagctcc
      301 tcgttctcgc gccggaggcg ttccagctcc tgggcttcag acgggctcaa agacttccgg
      361 ttggtctggg acatgccaca gcgctggacc atgtaccaga gcccaccacc attacccttc
      421 ctgctatccc actccagcca atcccctccc gcaaaatggc caaactcaag gggtcggccg
      481 tcaggccgac cgaccaagac taggcagggc gctcggcgcg acattcgcga gaccccgtcg
      541 ctcgaccaag agccctatgt gagcgcctat ggggcagcgc cccgcggtgc gccgccgccg
      601 aggtgtggtc tcctactctg cggcccgctg cccccgaagc gcttcattgg ttacctcgac
      661 gagcgcctca cggaccgcgg cgcgcagcga cctccgcacc gcaccgtcct gcagtgtttc
      721 ccggagtgcc atgcgcaggt gctcgcgcat cgcatcgctc tgcgccacct cgtcagcgac
      781 ctctgacacg gcctgccgca cccggtcctg cacggcatcg tccgtcagag ccgatccgac
      841 ggcgtctgac agcgactggc ggaccgcctt gtcattggcc acgtcgtgca cggccgagcg
      901 gatggccgaa ccaacgatgg tctcgacgcc accagcccgc aacaggacaa gtcccgtgat
      961 ggccagcagc acgagtgtca ccagcacgcc caggaagaac cacagcgcgg gggggcttgg
     1021 gtgtcgcgtt ggggcatcgg tcatggcgcg tctgccacct gcctgtctat gggcgggtcc
     1081 aacgtagcca gatcgcggcg gggggccgcc caggctcgga ccacggcatg gcgaactgcc
     1141 gacagcgttg cggcgtagtc cgggtcgtcg gcacgggcgt acgcggcggg ttggcagcgg
     1201 ccccacccgc ccgactcggt ggcgagtggc gccgccagga aacggctccg ggccgggtcg
     1261 cgaaggtcga tccagtccag ccgggcgacc tgttccggct cgtggcaggc cccacagcgt
     1321 cgtcggctca cgtccaccag cctcgcgcgc agggctgcat cggcggccag gtcgtagacg
     1381 gggcgttcgg gacgcttgtt gatgaaaccc gagtggtagg gcgcgttagc gtcgatccac
     1441 gtcacgaggc ggatccggtc atcctcgctc agccggacac ggccgcgatg ggtggtctcg
     1501 ataacctcga cgatccggct cttgttggag ccaaactcga gcggcggggt gatccgcgca
     1561 tcgtcgccca cgttggagcg cgccacgagc cgcgcgcggg tgatggtgtc ccacgcggtg
     1621 ttggccgtgg gggtgagtcc acccgagaag tcgtgaccac ctgcgggctg gagcccgctg
     1681 tggcagacca cgcagtggcg gtcgaacacc ggttggacgt cgcggaggaa gctgatgacg
     1741 ccgtcacccc atggcggcgg gacgagcggc gagggggccc gctgggcggc catcgggacg
     1801 gcggtgttcg gcggcgcggt gcctcgcgtc tcgtggcacc cggagcacga gcggcgctca
     1861 cctggctgga aactgatgaa ggaccgcatg cgccggagct ccatcatccg ctcgtccagg
     1921 gcctggaagt agacaggcgt gtcgggcggc acctcgaagt gcgcactccc atcgagttcc
     1981 acggggacgg tgcccagcac gcgcacgggg ttccagttga tcatcaccgg tttgacatcc
     2041 ggctcgtagc ggagcccccc gagtgccagg tcgtagggcc aggccacgcc ctcggcgatc
     2101 cgcaggtact tgacgcgccc gggctcgatc ccgtccattc ccatcgtcac gtcggtgacc
     2161 aggcatgatg ccgccctcgc ccccgggtcc caaaccgacg cgatgatagg gggacgcgtc
     2221 cggggccgga gtgggatcgg gtagaagcac gagatgtctg ggtcgcggta gaccaactcc
     2281 cgcgtgccgt atacgtccag cagatagagc ccatagccct tctcgtccgt catgacatcg
     2341 ctgtaggtga aactggcaag acatgccttc tcggacagcg gccatggcgt ggcatagagg
     2401 cctccgccgc cgggtactcc cccctcgggc accggtcgcc cggccatgcc cccctcaggc
     2461 ggactggtgc ccggcgtgat gatgccgatg ccccgcgggt cgttgatccc catctttggg
     2521 tcgacgagga ccaccggccc cacgggcagt gtgtggtgtc cggtggcgat ggccaggagc
     2581 ttgttgctgc cggggatcga atgcgcctcc tcgatggccc acgggtcgtt gaagtgctgc
     2641 ttgtacagcg cgtctgctcc cgtgccgtcg gggttcatca cccagaccga ctggatgtgg
     2701 gcccagttgc gctcctggta ctcccagcgc gtgtaggtga gcaggccgtt gtcgagggcg
     2761 tgaggcaggt agtccccgtc cttgttggcg ctcatgcggc ggat
//
LOCUS       DAOSPL010000519         2802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_35929_length_2802_cov_3.030215, whole genome shotgun sequence.
ACCESSION   DAOSPL010000519 DAOSPL010000000
VERSION     DAOSPL010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35929_length_2802_cov_3.030215"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLQ54_10110"
     CDS             complement(<1..322)
                     /locus_tag="PLQ54_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93656.1"
                     /translation="MQFEFLVEGESDEIAVRELAARALGPDHAVEVRRFQGKEDLLAR
                     WPERAAAYARIADPNRRIVVVLDNDRGNCIDLRRRVEAVARTAGLQVDGPLTARQVVV
                     RIAMH"
     gene            complement(312..1562)
                     /locus_tag="PLQ54_10115"
     CDS             complement(312..1562)
                     /locus_tag="PLQ54_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS93657.1"
                     /translation="MPDDRPPAPRIEELRVRNYRALRDLRLGKLSPLTVLLGPNGSGK
                     STVFDVFAFLSECLSEDLHGPWGRRGGLRELRSREAEGPVSVQLSYREPGMSLMTYLL
                     EIDEDAGAPVVRREVLRWTRDPGAGRPYHFLDVSKGAGHVVTGTDPGLAGARRPVALT
                     SSSRLAISALGQLREHPRVAALRDFITGWYLSYLSASDARHPPETGPQPRLNKKGDNL
                     ANVIQYLRERYPRVLERILATLCQRVPRLESVDAKTMEDGRLLLTIKDSPFTQPILAR
                     FASDGTLKMLAYLTVLHDPSPRPLVGIEEPENHLHPRLLPGLAAECAAATERSQLLVT
                     THSPFFINELRPEDVWVLERDAQGYARARVTSKMPGIREHIDAGGKLGHLWTEGYFNF
                     GDPLSRAGGSTPSPRRSRGAGRAV"
     gene            1709..>2802
                     /locus_tag="PLQ54_10120"
     CDS             1709..>2802
                     /locus_tag="PLQ54_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93658.1"
                     /translation="MRSATLCLLMACALLVGRPAWPATVYVAPDGDDTARGGLRDPFA
                     TVERAKAEVRRIRAGGLTEPVNVVLREGVYRITEPIVFEPEDSGTPECPITYGAYPGE
                     HPVISGGRPIRGRIRDVSGRFVAELPEAAGGKWVFRQLFIDGKRRTLARSPNEGYYHM
                     TSPAPPAADPATGQEVDRSHMAFAFRPGDIRKWPDLAQADVVVYHIWETAILPIASVD
                     ERASIVTFTGPSKWPFGWAGKQRFVVENVGAALDAPGEWLLFRRAGVLSVLPLPGEDL
                     TRAEIIAPVAPQLVRLAGDPDNGQFVEHLRFEGLSFQHAEWALPPEGHGDWQAAVTVD
                     AAIQARGARHCVIDRCEVAHVGGYGIWFERG"
BASE COUNT          420 a         1003 c          937 g          442 t
ORIGIN      
        1 catgcatggc gatgcgcacg accacctgac gcgccgtcaa cggaccatcc acctggagcc
       61 cagccgtgcg ggccacggcc tcgacccgtc gcctcaggtc gatgcagtta ccacggtcgt
      121 tatccagcac gacgacgatg cgccggttcg ggtcggcgat ccgcgcatag gccgccgccc
      181 tctccggcca ccgggccagc aagtcctcct tgccctggaa acggcgcacc tcgaccgcat
      241 ggtcagggcc gagcgcccga gccgccaact cgcgcacagc gatctcatcg gactcgccct
      301 cgacgaggaa ctcaaactgc acgcccggca cccctcgacc ggcgaggcga gggcgtcgaa
      361 ccgcccgccc gactcagcgg gtcgccgaag ttgaagtagc cctccgtcca gaggtggccc
      421 aacttgcccc cggcgtcgat gtgctcgcgt atgccgggca tcttggacgt cactcgggct
      481 cgcgcgtacc cctgtgcgtc gcgttcgagc acccaaacat cctcggggcg gagctcgttg
      541 atgaagaagg ggctgtgggt cgtcactaag agctgcgacc gctcggtcgc cgcagcacac
      601 tccgcggcca gccccggaag caggcgcgga tgtaggtggt tctcaggctc ctcgatgccc
      661 accagtggtc ggggggaggg gtcatgcagc accgtgaggt aggccagcat cttcagcgtg
      721 ccatcggagg cgaaccgggc gaggatgggc tgggtgaagg gggaatcctt gatggtgagg
      781 agcagccgcc catcctccat cgtcttcgcg tcgacggact ccagccgtgg gactcgctgg
      841 cacagcgtcg ccaggatccg ctcgagcact cggggatacc gctcccgtag gtactggatc
      901 acgttcgcca ggttgtcgcc cttcttgttg agcctcggct gggggccggt ctccggcggg
      961 tgacgggcgt cgctcgccga cagataggac agataccaac cggtgatgaa gtcccgcagg
     1021 gccgcgactc gcgggtgctc tcgtagctgc ccgagtgcgc tgatcgccag ccggctcgac
     1081 gacgtaaggg caactggcct ccgcgctccc gccagcccgg ggtccgtgcc ggtgacgaca
     1141 tgaccagcgc ccttgctgac atctaggaag tgatacggcc tgccagcccc cgggtctctt
     1201 gtccagcgga gcacctcccg tcgcacaacg ggcgcgcccg cgtcctcatc gatctcgagc
     1261 agataggtca tcaggctcat gcctggctct cggtagctca actggacgct gaccgggccc
     1321 tcggcctcgc ggctccgtag ttccctcaac ccgccacgtc tgccccatgg cccgtgcaga
     1381 tcctctgaga ggcactcgga gaggaacgcg aacacgtcga acaccgtcga cttgccgctg
     1441 ccattcggcc cgagcaacac ggtgagaggg ctgagcttgc ccagcctcag gtcgcgcagg
     1501 gctcggtagt tgcgcacgcg gagctcctcg atgcgcggtg cgggtggtcg gtcgtctggc
     1561 acgcgagccc tcccctccag ttcgtcccga gcgggacgca tggtcttctt cggcgctccc
     1621 accgccaccg tcctccctcg tcccccttgc aggcctctcc gcgcgccgcc gggaatccac
     1681 tcccacgaca tgccgggagg cgatcctcgt gcgatccgcg accctgtgct tgctaatggc
     1741 ctgtgccctg ctcgtcggca ggccggcctg gcccgccacg gtctacgtgg cgcccgatgg
     1801 cgacgatacc gcccgcggcg gcctgcgcga ccccttcgcc accgtcgagc gcgccaaggc
     1861 cgaggtgcgc cgaatcaggg ccggcggcct caccgaaccc gtgaacgtgg tccttcggga
     1921 gggcgtctac cgcatcaccg agcccatcgt gttcgagccc gaggactccg gcacgcccga
     1981 gtgccccatc acctacggcg cctatcccgg cgagcacccg gtcatctcgg gcgggcggcc
     2041 gatccgaggc cgcattcgcg atgtcagtgg ccgtttcgtc gccgaactac ccgaggcggc
     2101 cggtggaaag tgggtcttcc gccagctctt catcgacggg aagcgccgca cgctcgcccg
     2161 gtcgcccaac gagggctact accacatgac cagtccggcg ccgccggcgg cagacccggc
     2221 cacggggcag gaggtggatc ggtcccacat ggccttcgcg ttccgcccgg gcgatatccg
     2281 gaagtggccc gacctggccc aggccgatgt cgttgtctac cacatctggg agacggccat
     2341 cctgcccatc gcctcggtcg acgagcgagc gagtatcgtc accttcacgg ggccatcgaa
     2401 gtggcccttc ggctgggccg ggaagcagcg gttcgtggtg gagaacgtcg gcgccgccct
     2461 cgatgcgccg ggcgagtggc tcctgttccg acgggcgggc gtgctgagcg tcttgccgct
     2521 gccgggggag gacctgacgc gggcggagat cattgccccg gtggcgccgc aactggtccg
     2581 cctcgcaggc gaccctgaca acgggcagtt cgtcgagcac ctgaggttcg agggcctctc
     2641 gttccagcac gcggaatggg cactcccacc tgagggccac ggcgactggc aggcggcggt
     2701 cacggtggac gcggcgatcc aggcccgcgg ggcccgccac tgtgtcatcg accggtgcga
     2761 ggtcgcccat gtgggtgggt acggcatctg gttcgagcga gg
//
LOCUS       DAOSPL010000520         2793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_36063_length_2793_cov_3.305698, whole genome shotgun sequence.
ACCESSION   DAOSPL010000520 DAOSPL010000000
VERSION     DAOSPL010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36063_length_2793_cov_3.305698"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PLQ54_10125"
     CDS             complement(<1..644)
                     /locus_tag="PLQ54_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOS93659.1"
                     /translation="MEQAGVDVGAAPDYRVFRVGVLDTNCYLVWCTDTKHAVLIDPGE
                     SPEPALAEAERLGLRIGCVLATHGHFDHVMGVNEVWERADGVPFLLHPADHAMALAPS
                     AQAEFVLGRPVPSVCPTGALEDGQVIEFGRCRLRVIHTPGHTQGGVCLSTDGMIFTGD
                     TLFCGSVGRTDFEGGDWDQLMASIRDRLLPLPDGTRVLPGHGPETRIGDERETNP"
     gene            complement(620..1552)
                     /locus_tag="PLQ54_10130"
     CDS             complement(620..1552)
                     /locus_tag="PLQ54_10130"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS93660.1"
                     /translation="MREDQEPEESTEESPEEQSQAPAEAGDGPDDGFVFEVLSELPAA
                     GGFRAEVYGQRGDALYEAGLVERALQDYREAARLGRSAKWRRRVADSYFALGLPQQAY
                     RAYRRAIQLDPKDPESHFFMGEFLQSMSRTFWAIEEFREALRLSGADQPYYALKLGEA
                     CLAVGLLKDAVEALTLAARRKPGDSYYRFRLANALIRSGALEDAVRQLEHAVRLSPCD
                     DYYHALLAMAYKGSSRPHDALRAIRRAAEIRPRNRAYRHLVAEACQEAGLTTLSRRFR
                     DLAGRLDEYDADYVDRLKNKVLGGAGEWNRPEWM"
     gene            complement(1569..2018)
                     /gene="dtd"
                     /locus_tag="PLQ54_10135"
     CDS             complement(1569..2018)
                     /gene="dtd"
                     /locus_tag="PLQ54_10135"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOS93661.1"
                     /translation="MRAVLQRVTEARVTVEGEVVGEIGPGIVVLLGVARDDTERDADY
                     LAEKVANLRVFEGPGGKLDRPIADVAGSGILVVPNFTICADARRGRRPDLASAARPEK
                     AESLCDRFASRAAAMGVRVATGRFGAHMHVELTNDGPVTLVVESPGR"
     gene            complement(2015..>2793)
                     /locus_tag="PLQ54_10140"
     CDS             complement(2015..>2793)
                     /locus_tag="PLQ54_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93662.1"
                     /translation="SARAKIRAYFRRVTFEENLERGRELLQQEARQRFGTDWQDTVTA
                     DRLSATAAELNYPSADRLLAGIGFREVSAEAVLGKIDNAILKEQLQSLESIGLLAKHR
                     PVRDGAVQIIASGMDGLRFRLSRCCGPLPGDPIVGYVTRGRGLAIHRSDCHNARALMA
                     KDPPRILPCEWAESSEEGAHLGHIELEAIERVGLLKDITSIVADVGVTVNEANIRSTD
                     GGRKAAMDLVFTVSGAEQVNALVDRFSQLSDVLRVRRVPK"
BASE COUNT          454 a          985 c          891 g          463 t
ORIGIN      
        1 ggattggtct cccgctcatc gccgatccgc gtctccgggc cgtgaccggg gagtacgcgc
       61 gtgccgtcgg gcagcggcag cagccggtcg cggatcgatg ccatcagctg gtcccagtca
      121 ccgccttcga agtccgtgcg gcccacactc ccacagaaca gcgtgtcgcc ggtgaagatc
      181 atgccgtcag tggacaggca gacgccgccc tgggtgtggc cgggagtgtg aatcacgcgg
      241 agccggcagc gcccgaactc gatgacctgc ccatcctcga gcgcgccagt cgggcacacc
      301 gagggtaccg gccggcccag gacgaactca gcctgcgccg atggagccag ggccatggcg
      361 tggtcggccg ggtgcagcag gaagggtacc ccatcggccc gctcccacac ctcgtttacg
      421 cccatgacgt ggtcgaagtg cccgtgggtg gccaggacac acccgatgcg gagccccaag
      481 cgctcggctt cggccagggc cggttcgggc gactcgcctg ggtcgatcag gaccgcgtgc
      541 ttcgtgtcgg tgcaccatac caggtagcag ttggtgtcca agaccccgac tcggaacacg
      601 cggtagtccg gtgccgcgcc tacatccact ccggcctgtt ccactcgcca gcaccaccca
      661 acaccttgtt cttgaggcga tcgacgtagt ctgcgtcgta ctcgtccagg cgtcccgcca
      721 gatcccggaa acggcgcgac agggtggtga gcccggcctc ttggcaggcc tcagcgacca
      781 agtggcggta ggcccggttc ctcggccgga tctccgccgc gcggcggatg gccctcagcg
      841 cgtcatgggg ccggctggat cccttgtagg ccatggcgag tagcgcatgg tagtagtcgt
      901 cacacgggga gagccgaacg gcatgctcaa gctgtctcac cgcgtcctcc aaagcaccag
      961 agcggatgag cgcgttggcc aggcggaagc gatagtagga gtcgccgggc ttccgccgcg
     1021 ccgccagcgt gagcgcctcg accgcatcct tcaagagccc gaccgccaag catgcctcgc
     1081 ccaacttgag cgcatagtac ggctggtccg cgcccgagag cctcagcgcc tcgcgaaact
     1141 cctcgatggc ccagaacgtg cgactcatgc tctggaggaa ctcacccata aagaaatggg
     1201 actctgggtc cttggggtca agttggatgg cgcgtctgta ggcgcggtaa gcctgttggg
     1261 gaagtccgag ggcgaaatag gagtccgcaa cacgccggcg ccacttggcg ctgcggccga
     1321 gccgcgccgc ctcccggtag tcctggaggg cacgttctac gagtccggcc tcatagaggg
     1381 cgtcgccccg ctgtccgtat acttcggccc ggaagccccc ggcagcgggg agctcggaga
     1441 gcacctcgaa aacgaaacca tcgtcggggc catctccagc ctccgctggt gcctggctct
     1501 gctcctccgg agactcctcg gtgctctcct ctggctcctg gtcctcgcgc atccccatgt
     1561 tctcgtgtct acctacccgg gctctccacg accagtgtca ccggtccgtc gttggtgagt
     1621 tcgacatgca tatgggctcc gaagcgcccc gtggccacac gcacccccat ggcggccgcc
     1681 cggctggcga agcggtcgca cagggactcg gccttttcgg ggcgagccgc ggatgccaga
     1741 tctggccggc gtccacgacg ggcatcggcg caaatagtaa agtttggcac aacaaggatt
     1801 ccgctgcccg cgacgtcggc gatcggccga tccagtttgc ccccggggcc ctcgaacacc
     1861 cggaggttcg cgaccttctc cgccaggtag tccgcatcgc gttcggtgtc gtctcgagcc
     1921 acaccgagca acaccacgat gcccgggccg atctctccca cgacttcgcc ctcgaccgtg
     1981 acacgcgcct cggtcacgcg ctgcaggacc gccctcactt gggcacccgc cgcacacgca
     2041 agacgtccga gagctgggag aacctgtcga ccagcgcgtt gacctgttcg gccccgctga
     2101 cggtgaacac caggtccatc gcggctttcc tgcccccgtc ggtgctgcgg atgttggcct
     2161 cgttcacggt gacacctacg tccgccacaa tagaggtgat atccttgagc agacccaccc
     2221 gctcgatggc ctcgagttcg atgtgcccca ggtgcgcgcc ctcttcgcta ctttctgccc
     2281 actcacacgg taggatccgt ggaggatcct tggccatgag cgcccgcgca ttgtggcagt
     2341 ccgaccggtg gatcgcaagc ccccggcccc gggtcacgta cccgacaatt gggtctccgg
     2401 gcaacgggcc gcaacaccgc gacaggcgga atcggaggcc gtccatgccc gaggcaatga
     2461 tctgcaccgc accgtcgcgc accggccggt gcttggccag caggccgatg gactcgaggc
     2521 tttgcagctg ctccttgagg atggcgttgt cgatcttccc gaggaccgct tccgcggaca
     2581 cttcgcggaa cccgatcccg gccaacaggc ggtcggccga tggatagttg agctccgcgg
     2641 ccgtcgcgct cagccgatcc gccgtgaccg tgtcctgcca gtcggtgcca aaacgctgcc
     2701 gggcctcctg ttgcagcagt tcgcggccac gctccaggtt ctcctcgaac gtgacccggc
     2761 ggaagtaggc ccggatcttg gcacgagccg agg
//
LOCUS       DAOSPL010000521         2789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_36134_length_2789_cov_3.858815, whole genome shotgun sequence.
ACCESSION   DAOSPL010000521 DAOSPL010000000
VERSION     DAOSPL010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36134_length_2789_cov_3.858815"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PLQ54_10145"
     CDS             complement(<1..787)
                     /locus_tag="PLQ54_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009377366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HOS93663.1"
                     /translation="MTTGSATVTLRTDARIGRIHPNLYGHFAEHLGRCIYEGLWVGED
                     SAIPHLRGIRQDTVCLLRELGAPVIRWPGGCFADDYHWEDGVGPRDQRPRRLNLWWPA
                     EESNHFGTHEFIDVCRQVGAEPYICLNVGSGSPREARDWIEYCNAEGNTTLANRRRAH
                     GHSQPFRVRYFGVGNENWGCGGQFDPVDYAKEYRRYACFLRAVGQDVQLIACGHTTAD
                     WNLRFLEAAEGASIDHLSIHRYYGSGPSTGFSEADHYQLVARAP"
     gene            complement(934..2397)
                     /locus_tag="PLQ54_10150"
     CDS             complement(934..2397)
                     /locus_tag="PLQ54_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93664.1"
                     /translation="MRTRLMVLGLLLLGVGWVSSPSWAQERVTGRVYLPVNDDAELGE
                     ILLFDIIAQANSMPLIDVLDLHNEFDLYPEEVMMVLFLAHEGREDADHVAEMRADKRY
                     AWKQIAQREKIKPEVLGQPDKWYSRGDKDEWWREDPEVERVVMLDMLAAAFGVDIRAV
                     EQWSNDGLGYSDIALALELERRSGKSADELLYAKTNQEVPWSRLADDLGVDLEDLYIK
                     RSLHYHDASFQPIIPGDRRIYHGRRLDRDHWSRREYHYYGVVFVDPFDYAWDYDRYFP
                     YGRPGRFVRWPDDRWHYRPPLLEWAGPYGHGVYHPPYRPRPGFDWPGGHRPGGYYGRP
                     DDGRDGRPPAVPPTQRDTRRYSEKLNDRGTSSVVVPGGRQVRGNDPGPARQPSSGSSQ
                     SSERQRLRDRQREAEQAERARRVAPSPPPSSRDSQPKQQVQPRPESRPKEQARPSAPP
                     SRPQPSAPSGRSGGSNGSNGGNGGSGGGGGGARGGRR"
     gene            2608..>2789
                     /locus_tag="PLQ54_10155"
     CDS             2608..>2789
                     /locus_tag="PLQ54_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93665.1"
                     /translation="MQDPDSNVAPRTDRELAKTPPRGITLRSLLIGFALMPLNMYWMV
                     QIEEVRWAGYPTTVSL"
BASE COUNT          464 a          960 c          906 g          459 t
ORIGIN      
        1 ggggcgcccg ggcgacaagc tggtagtgat cggcctcgct gaagcccgta gagggccccg
       61 acccgtagta gcgatggatg gacagatggt cgatgctggc accctcggcg gcctcgagga
      121 agcggaggtt ccagtccgcg gtcgtatggc cacaggcgat gagctggaca tcctggccca
      181 cggcgcggag aaagcaggcg tagcgccggt actccttggc atagtccacc gggtcgaact
      241 ggccgccgca gccccagttc tcgttgccga caccgaagta gcggacccgg aagggctgcg
      301 aatgaccgtg ggcccggcgc cggttcgcca gcgtggtgtt gccctcggcg ttgcagtatt
      361 cgatccaatc acgtgcctca cgaggggagc ctgaaccgac gttgaggcag atgtagggct
      421 ccgcaccgac ctgtcggcag acatcgatga actcatgggt gccgaagtga ttgctctcct
      481 cggcgggcca ccagaggttg agacgccgcg ggcgttggtc gcgcggccca acgccgtctt
      541 cccagtgata gtcgtcggcg aagcaccctc ccggccagcg gatcacaggg gcgccaagct
      601 cgcggagcag gcacacggtg tcctggcgga tcccacgaag gtgggggatc gccgagtcct
      661 cgcccaccca caggccctca tagatgcagc ggcccaggtg ttcggcgaag tggccgtaga
      721 gattcgggtg gatacgcccg atccgagcgt cggttcgcag cgtgaccgtg gcagagccgg
      781 ttgtcatgtg gcggcacctc ggcaagggaa tggccgggtg ttcctcaggt gcccgggccg
      841 ctcctgcgaa agggggtgcc acgcgatagc ggggctccct ggctcccagg aagccccgct
      901 cgaatctgcg cccacgtcag ggcaaacgat gctctaccgg cggccacccc tcgcgccgcc
      961 accaccgcca ccgctgccgc cgttgccgcc gttgctgccg ttgctgccgc cggatcgccc
     1021 gctgggcgcc gagggttgcg gccggctcgg cggcgcgctg ggacgcgcct gctccttggg
     1081 tcgcgactcg ggccgcggct gcacctgctg cttgggctgc gaatcccggc tgctcggcgg
     1141 cgggctcggg gccacccggc gcgcccgctc ggcctgttca gcctcgcgct gccgatccct
     1201 cagccgctgc cgctccgagc tctgggatga tccagacgaa ggctgcctgg ccggacccgg
     1261 atcgttaccc ctcacctggc gtccgcccgg gacgaccacc gagctcgtgc cgcggtcgtt
     1321 gagtttctcg gagtaacgac gtgtatccct ctgggtcggg ggcacagccg gcggcctgcc
     1381 gtcacgaccg tcatcggggc gcccgtagta gccgcccggg cggtgcccgc cgggccagtc
     1441 gaaaccgggc ctcggtcggt acggcggatg gtagacccca tgtccgtatg gacccgccca
     1501 ctccaacagt ggagggcggt agtgccagcg atcatccggc caccgcacga agcgtcctgg
     1561 ccggccatac gggaagtagc ggtcgtagtc ccacgcgtag tcaaacgggt ccacaaagac
     1621 cacgccatag tagtggtact cgcggcgtga ccagtggtcg cggtccagcc gtcgcccatg
     1681 gtagatgcgc cgatctccag ggatgatggg ctggaagctc gcatcgtggt agtgcaagct
     1741 gcgtttgatg tacagatcct cgaggtccac cccaaggtca tcggcgaggc ggctccaggg
     1801 cacctcctgg ttcgtcttgg cgtagagcaa ctcatcggcc gacttgcccg aacgccgctc
     1861 cagctcgagc gccaacgcga tgtcggagta gccgagccca tcgttgctcc actgctccac
     1921 ggcgcggatg tccacaccga aggccgccgc gagcatgtcc agcatgacca ctcgctcgac
     1981 ctcggggtct tcacgccacc actcgtcctt gtcgccgcgg ctataccact tgtcgggctg
     2041 gcccagcacc tcgggcttga tcttctccct ctgcgcgatc tgcttccacg cgtagcgctt
     2101 gtcagcccgc atctcggcca catggtcggc gtcttcccgg ccttcgtgag ccaggaacag
     2161 aaccatcatg acctcttcgg gatagaggtc gaactcattg tgcagatcga gcacgtcaat
     2221 cagaggcatg gagttggcct gagcgatgat atcgaagagg aggatctcgc ctagctccgc
     2281 gtcatcattc accggcaggt acacccgccc ggtgacccgc tcctgcgccc atgagggcga
     2341 ggacacccac ccaaccccga ggaggagtag ccccagcacc atcaatcgcg tccgcatacg
     2401 tccgacctcc tctcgccaag ccacgagcat caatgaagaa ccatgggcag acgaccccga
     2461 atccattatc cgtctttctc catgacgaag cacgccgcct gcgggttccg cccggacggc
     2521 tcagcaggcc cgcagcgact tgggtgggaa cacccaggcg accaagcacg agctgtcgaa
     2581 cggcgctcca ggaggatcag cgcaaccttg caggatccag actcgaacgt ggcgccgcga
     2641 accgatcgcg agttggcgaa gaccccgccc aggggaatca ctctccgctc gctgctcatc
     2701 gggttcgcgc tgatgcccct gaacatgtac tggatggtcc agatcgagga ggtccgctgg
     2761 gccggctatc ccaccacggt ctccctgtt
//
LOCUS       DAOSPL010000522         2789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_36140_length_2789_cov_2.937820, whole genome shotgun sequence.
ACCESSION   DAOSPL010000522 DAOSPL010000000
VERSION     DAOSPL010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36140_length_2789_cov_2.937820"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..1428
                     /gene="pyrB"
                     /locus_tag="PLQ54_10160"
     CDS             265..1428
                     /gene="pyrB"
                     /locus_tag="PLQ54_10160"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF002032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOS93666.1"
                     /translation="MKNLVSIRDLPNQDLLRIFELAEAFWRHRSADPDQNVELANQFI
                     AAHGLGRPFTRDRVLATLFFEPSTRTRLSFESAMHRLGGRVIGFSDARTSSVSKGETV
                     ADTIRMVSGYADIIAMRHPAEGSAFVGACFATRPIINAGDGGREHPTQCLLDLFTLQR
                     ELGRIEGLKVGLLGDLRFGRTVHSLAQGLSRLGAEIICIAPESMRMPDRFVQFVEEAT
                     GRPPLEVVRPEEVIGELDALYVTRIQAERFEDPDEYEAMKGMYIVDRRLMDQARDSCI
                     IMHPLPRVDEVSTELDGDPRSAYIRQSHYGVPVRMALMVLLLASAAEDAAETGIPADP
                     NPELGVAARPCDNPRCILRVERSLPARFFRSRDSSIRCGYCEAVPQQRRLELR"
     gene            1425..2219
                     /locus_tag="PLQ54_10165"
     CDS             1425..2219
                     /locus_tag="PLQ54_10165"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOS93667.1"
                     /translation="MSEGAPRPGQDHYVAQVRTNIGVADGHRLLTLICPEVAQTAVPG
                     QFLQIRVSVSGQDPLLRRPICVAAAREDEVDIVVRVAGRGTDLLAREAPGAFVDLLGP
                     LGRGFTRPGRHEPVALVGGGVGVAPLHFLLTSLADPSRVRVLLGARTRALLPRLPGLS
                     GLEGLRIATDDGSEGHHGTAADLLAGVLETDPPHRVYACGPQPMLAAVARLCIQRRIV
                     CEVSLEERMACGIGVCMGCAVPMAGGGYTRACREGPVYDARAVKWE"
     gene            2216..>2789
                     /locus_tag="PLQ54_10170"
     CDS             2216..>2789
                     /locus_tag="PLQ54_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOS93668.1"
                     /translation="MTPDLSVRLGPLTLRNPVMVASGCCGFGQELAEWMPLSALGAIV
                     VKGTTLRPRRGNPPPRVWETPAGMLNAIGLQNDGVQDLIEHKLPWLREQDVPIIVNIA
                     GETVGEFAELARVLDRAPGVAALELNVSCPNVRAGGMQFGVCATGVAEVTSAAREATR
                     LPIITKLSPNVTDIVEMARAAVDAGADALSL"
BASE COUNT          387 a         1011 c          945 g          446 t
ORIGIN      
        1 gcacgtctgg atctggcgga ggggttacat ggggaggacc gtccggcgaa gccggatggt
       61 gggcccccag tgggcccggg tcggccgcca ggccgaccga ccaagaccgt cagcactctc
      121 cgactgcgct ctccggcccg tccagggccg tcgggcgccg gacccgagta ggactccgcc
      181 gcggcggctg cgaaacaccc tccggccggg ggatgcctcg gttgccattg cccggccctg
      241 tgccgaccga gggggtgtgc gcgcgtgaag aacctcgtct cgatcaggga cctgcccaac
      301 caggacctgc tgaggatctt cgagctggcc gaagcgttct ggcggcaccg gagcgctgac
      361 cccgaccaga acgtcgagct cgcaaaccag ttcattgccg cccacggtct tggtcggccc
      421 ttcacccgcg accgcgtgct cgccaccctg ttcttcgagc cgagcactcg cacgcgactc
      481 tcgttcgagt ccgccatgca tcgtctgggc ggccgcgtca tcgggttctc cgacgcgcgg
      541 accagctccg tctccaaggg agagacggtc gctgacacga tccgcatggt cagcggctac
      601 gccgacatca ttgccatgcg ccacccggcc gaagggtcgg ccttcgtggg cgcgtgcttc
      661 gccacccggc ccatcatcaa cgcgggcgat ggcggccggg agcacccgac ccagtgcctg
      721 ctcgacctct tcaccctcca gcgcgaactg gggcgcatcg aggggctcaa ggtcgggctg
      781 ctcggcgatc tgcgcttcgg ccggaccgtc cactcgctgg cccagggcct ctcccggctc
      841 ggcgctgaga tcatctgcat cgccccggag tccatgcgga tgcccgatcg gttcgtgcag
      901 ttcgtcgagg aggccaccgg ccgccccccc ctcgaggtgg tccgccccga ggaggtcatc
      961 ggcgagctgg atgcgctcta cgtcacccgc atccaggccg agcggttcga ggatcctgac
     1021 gagtacgagg ccatgaaggg catgtacatc gtcgatcggc gcctcatgga ccaggcgcgc
     1081 gactcgtgta tcatcatgca cccgctgccg cgcgtcgacg aggtctcgac tgagctagat
     1141 ggcgacccac ggtcggccta catccgccag agccactacg gcgtccccgt tcgcatggcg
     1201 ctgatggtgc tgctgctggc gtcggccgca gaggatgccg ccgaaaccgg catcccagcc
     1261 gacccgaatc ccgaactcgg ggtcgcggcg cgtccgtgtg acaaccctcg gtgcatcctc
     1321 cgcgtcgaac gctcactccc agcgcgtttc ttccggtcgc gcgactcttc catccgctgc
     1381 ggctactgcg aggccgtgcc tcagcagcgc cgactggagc tccgatgagt gagggcgcgc
     1441 ccaggcccgg ccaggaccac tatgtcgccc aggtgcgaac caacatcggg gtggccgatg
     1501 gccatcgcct cctcacgttg atctgccccg aggtagctca gacggcggtg cccggccagt
     1561 ttctgcagat ccgggtctcg gtctcgggcc aagacccgct gctccggcgg ccgatctgtg
     1621 tcgcggcggc gcgcgaggat gaggtcgata ttgtggtccg cgtcgccggc cgcggaaccg
     1681 acttgctcgc ccgcgaggcg cccggggcgt tcgtcgacct acttggccct cttggccggg
     1741 ggttcacacg accagggcgc cacgagcccg tcgcgctggt gggaggcgga gtcggtgtgg
     1801 cgccgctcca cttcctcctt acctcgctgg cggatccatc gcgcgttcga gtgctgctgg
     1861 gggcccgtac gcgcgcgctc ctgccgcgcc tgcccggcct gtcgggcctg gagggactac
     1921 ggatcgcgac cgacgacgga tctgagggcc accacgggac cgctgcggac ctcctggcgg
     1981 gcgtgctcga gaccgatccc ccccatcgcg tctacgcatg cgggcctcag ccgatgctgg
     2041 ccgcggtggc ccgcctgtgc atccaacgcc gaatcgtctg cgaggtgtcg ctcgaggaac
     2101 gcatggcctg cgggatcggg gtgtgcatgg gctgtgccgt ccccatggcc gggggcggct
     2161 acacgcgcgc gtgccgcgag ggccccgtct acgacgccag ggcggtgaag tgggagtgac
     2221 ccccgacctg tcggtccgcc tcgggccgct cacacttcgt aacccggtga tggtcgcatc
     2281 gggctgctgc ggattcggcc aggaactggc cgagtggatg cccctctccg ccctgggcgc
     2341 catcgtggtg aagggcacga cgctgcgccc gcgccgcggc aacccacccc cccgcgtctg
     2401 ggagaccccc gcgggaatgc tcaacgctat cggccttcag aacgacgggg tccaggacct
     2461 gatcgagcac aagctgccct ggttgcggga gcaggatgtg cccatcatcg tcaacatcgc
     2521 cggcgaaacc gttggcgagt tcgccgagct cgcgcgggtc ctcgatcgcg cgcccggtgt
     2581 cgccgcgctc gaactcaacg tgtcgtgccc gaacgtccgg gcgggtggca tgcagttcgg
     2641 cgtctgtgcc acgggcgtgg ccgaggtgac ctccgccgct cgggaggcca cgcggctgcc
     2701 catcatcacc aagctgtcgc ccaacgtcac cgacatcgtc gagatggccc gggccgccgt
     2761 cgatgccggc gccgacgccc tttcgctga
//
LOCUS       DAOSPL010000523         2780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_36288_length_2780_cov_2.884771, whole genome shotgun sequence.
ACCESSION   DAOSPL010000523 DAOSPL010000000
VERSION     DAOSPL010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36288_length_2780_cov_2.884771"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLQ54_10175"
     CDS             complement(<1..397)
                     /locus_tag="PLQ54_10175"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOS93669.1"
                     /translation="MPVITISREYGSLGDEIGQGVAEQLSLRFVDKEVVSSAAELAGV
                     PEPELAEAYEKRPSFLERFVILGKAERYAGHMIQAIQRIAADGDVVLMGRGAQVLIEA
                     SPDVLHVRVVAPLDVRAERIAEAEGLEWLA"
     gene            572..1117
                     /locus_tag="PLQ54_10180"
     CDS             572..1117
                     /locus_tag="PLQ54_10180"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOS93670.1"
                     /translation="MRTTMLMFTLAIAASLWGCPKGSQPDGPSDTPPSPAKVTAPGSS
                     TAGATGQRGVPEADAAGSVAEGIQWVSDLDQAVRSAAAEKKPVMVDFYTDWCSWCKKL
                     DSDTYTDTEVQKLAARFVNAKVDAEADRAAAEKYQVDGFPTIVFLSPDGEEIHRIGGY
                     APPEKFLKEMQTALDKVKQGS"
     gene            complement(1101..2021)
                     /locus_tag="PLQ54_10185"
     CDS             complement(1101..2021)
                     /locus_tag="PLQ54_10185"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOS93671.1"
                     /translation="MRIGIVAPSCPPTPQRLDAGVRALRHQGFEVVVSHEVLRPRPFT
                     AADDQMRTEAIADMLRREDIDALVCARGGFGAARLIPYLSEVGPLPHKPFMGFSDITV
                     LLLSWLRSGGPQPVWGPMVAVSLGDDAPPRAVELGRRALRGDLGGAELIGPDDHAQSM
                     VSGTVEAEVVGGTLCLCSESIGTPYELDCKERILLLEDQNEDLYRIDRYLMHLRLAGK
                     LDDACGFILGRTFVGDDVTGFARSLDELLADYLAPLGKPVLANVPTGHAEYCLPIPFG
                     VPVRMAVGGSPSEHRLTVLGGRSLALSSPA"
     gene            complement(2135..>2780)
                     /locus_tag="PLQ54_10190"
     CDS             complement(2135..>2780)
                     /locus_tag="PLQ54_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOS93672.1"
                     /translation="KDETTIVDGAGDSEAIEGRLNEIRKAIEETDSDYDREKLEERLA
                     KLAGGVAVIQVGAATETELKEKKHRFEDALSATRAAVEEGIVPGGGTCLLRCQQAVDK
                     LKLKGDEATGAAIVRAALERPLRSIAENAGLEGSVVVEKVRTLGPTEGFNALTEQYED
                     LVKAGIVDPAKVVRSSLQNAASIAGMLLTTESLVANKPEEPKAGPPGGGGMPPY"
BASE COUNT          464 a          908 c          952 g          456 t
ORIGIN      
        1 cggccagcca ttcgagcccc tcggcttcgg caatgcgctc ggcgcggaca tcgagcggag
       61 ccacgacgcg cacatggagc acatccgggg aggcctcgat gaggacctgt gcgccccgcc
      121 ccatgagcac gacatcgccg tcggccgcga tccgctggat cgcctggatc atgtgcccgg
      181 catagcgctc ggccttgccc aggatcacga atcgctcgag gaaactgggc cgcttctcgt
      241 aggcctcggc cagctccggc tcgggaacac ccgccagctc ggccgcgctg gaaacgactt
      301 ccttgtcgac aaaccggagc gagagctgct cggcgactcc ctgcccgatt tcgtcgccca
      361 gactgccgta ttcccgagag atggtgatga caggcatcgt gaaccaccct cctcgcgccc
      421 aagggcgttc gtgtcctcca caccccttcg tcgcgcggat cgggtgattc ctgcaacaat
      481 atggcgcgta gtcggcacct ggggacccgc ccgagcgtca ttgtcaccag tcggctcaca
      541 cggtgcgcca ctgacagggg gaggaagctc gatgaggacg accatgctga tgttcacgct
      601 cgccattgcc gctagcctgt gggggtgccc gaaaggcagt cagcccgacg gcccaagcga
      661 cacaccgccg tcgcccgcga aggtgacggc gccggggagt agcacggccg gagccaccgg
      721 ccagaggggc gtgcctgagg cagacgcggc gggctcggtc gccgagggga tccagtgggt
      781 gagtgacctc gaccaagccg tccggagcgc cgcggccgag aagaagccgg ttatggtcga
      841 cttctacacg gactggtgca gctggtgcaa gaagctcgac tcggacacct ataccgacac
      901 ggaagtccag aagctggcgg cgcggttcgt caacgccaag gtcgacgccg aagcggaccg
      961 ggccgcagcc gagaagtacc aagtggacgg tttcccgacg atcgtcttcc tgtcgcccga
     1021 tggcgaggag atccaccgca tcgggggcta cgcgccaccc gagaagttcc tcaaggagat
     1081 gcagacggcg ctggacaagg tcaagcaggg gagctgaggg ccagactgcg accgccgagc
     1141 acggtgaggc ggtgctccga ggggcttccg cctaccgcca ttcgaacagg cacaccgaac
     1201 ggtatgggca ggcagtactc ggcatgcccg gtgggtacgt ttgcgagaac gggcttgccc
     1261 agtggtgcca ggtagtcggc gagtagctcg tccagactgc gggcgaagcc ggttacgtcg
     1321 tctccgacga acgtccggcc gaggatgaaa ccgcaggcgt cgtcgagctt ccccgcgagc
     1381 cggaggtgca tgaggtagcg gtcgatgcgg tagaggtcct cgttctggtc ctcgagcagg
     1441 aggatccgct ctttgcagtc gagttcgtat ggggtgccga tgctctcgga gcagagacac
     1501 agcgtgcccc cgaccacctc ggcctcaacg gtgccggaga ccatgctttg tgcgtggtca
     1561 tccgggccaa tcagctcggc gccgcccaga tcgccacgga gggctcgccg gccgagttcg
     1621 acggccctcg gtggagcgtc gtcgccgagg ctgacggcga ccatggggcc ccagaccggc
     1681 tgtgggcctc ccgatctgag ccacgacagg agcagcacgg tgatatcgga gaagcccatg
     1741 aatggcttgt gaggtagggg gccgacctcg ctcaggtagg ggatcagccg agcggcgcca
     1801 aagcctcctc gggcacagac cagtgcgtcg atgtcctcgc ggcggagcat gtctgcgatg
     1861 gcctctgtcc gcatttggtc atcggcggcc gtgaacggtc gtggccggag tacttcatgg
     1921 ctgaccacca cctcgaagcc ctggtgtcgg agagcccgca ctccggcatc gaggcgctgt
     1981 ggggtgggtg ggcacgaggg ggccacgatg ccgattctca cggaacacca cttccttggt
     2041 ctgctggaga cagcacggcc cgaaacgagc atcccgcgct cgagggtatc gaacgcggga
     2101 tgccagtacg tcatgctgac gggccatgcc cagcctagta gggcggcatg ccgcctccac
     2161 cgggcgggcc ggccttcggc tcttcgggct tgttggccac gaggctctcg gtcgtgagca
     2221 gcatccctgc gatgctcgcc gcgttctgca gggacgaccg gaccaccttg gccgggtcaa
     2281 cgataccggc cttgacgagg tcctcgtatt gctcggtgag cgcgttgaac ccctcggtgg
     2341 gaccaagggt gcggaccttc tcgaccacga cagatccctc gaggcccgcg ttctcggcga
     2401 tggatcgcaa ggggcgctcg agtgcggcgc gcacgatggc ggcaccggtc gcctcatcgc
     2461 ccttgagctt gagcttatcg acggcctgct ggcaccggag caggcaggtg ccaccgccgg
     2521 ggacgatgcc ctcttcgacg gcggcgcggg tggccgacag ggcgtcctcg aagcggtgct
     2581 tcttctcctt gagctcggtc tcggtggcgg cgcccacctg gatgaccgcg acgccaccgg
     2641 ccagtttggc caggcgctct tcgagcttct ctcggtcgta gtccgagtcg gtctcctcga
     2701 tggccttacg gatctcgttc agccggcctt cgatagcctc ggaatcgcct gcgccgtcga
     2761 cgatggtcgt ctcgtccttg
//
LOCUS       DAOSPL010000524         2779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_36302_length_2779_cov_3.085169, whole genome shotgun sequence.
ACCESSION   DAOSPL010000524 DAOSPL010000000
VERSION     DAOSPL010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36302_length_2779_cov_3.085169"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /gene="hisC"
                     /locus_tag="PLQ54_10195"
     CDS             <1..1375
                     /gene="hisC"
                     /locus_tag="PLQ54_10195"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOS93673.1"
                     /translation="RAGQGPRALWAHPCAAPPPDPDVPSRCSCGLISGLTWRQGGLAP
                     VPPFAAPMRLVYTSSPLAAAAVDAPEGVRALRWKGPSTVSEDARRLSEICPLVRPCLD
                     RLEAYRPGKPIEEVRRELGLSEVYKLASNENPLGPSREAIETMQRVLADAWLYPEDTC
                     FDLVRELSAHLGVPAERLIIGRGSDELIHLIGMAFVNPGEELIMADPPFPMYPITATQ
                     MDGWAVRVPLKDYTHDLPAMAAAITPKTKVIFIANPHNPTGTIVRKPELEAFLSRVPD
                     HALVVMDEAYCEFVDDPGYPTSLDYVAEGRNVVVLRTFSKIYALAGLRVGYGVFPDHL
                     APAVKRVRPPFNVSTVAQAAAVASLRDAGQVARSKAYNIEARRYLCGEFDRLGLAYAP
                     SQANFILVDLGVDCVEAFQRLLRRGYIVRTGDVFDLPTHVRVSFGTPEQNAGFIAALE
                     QVLAELR"
     gene            1388..2293
                     /locus_tag="PLQ54_10200"
     CDS             1388..2293
                     /locus_tag="PLQ54_10200"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HOS93674.1"
                     /translation="MAECFERVAILGVGLIGGSMGMALHSAGAAGMVVGCSRTESRLC
                     EAKRRGAIDSYTTDPLAAVDGADLVILCLPVASIPASARTIAPVLRSDAIVTDVGSAK
                     ACIVDALEELFDGRGQFVGGHPMAGSAQQGVWHARANLFHGATYFVTPTGRTPCRATD
                     AVTSLAHQLGARPVHLPPVDHDRVVAAISHLPHVVASGLASLVPELCGAFADVALEAA
                     AGSFADGTRVAGSDPSLWAEILVQNRAEVLRALASFRARLDAFESALSAGDEAATREL
                     FGLGTEVRRRFEAREARPEPPGQEG"
     gene            2296..>2779
                     /gene="cmk"
                     /locus_tag="PLQ54_10205"
     CDS             2296..>2779
                     /gene="cmk"
                     /locus_tag="PLQ54_10205"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOS93675.1"
                     /translation="MGSTSKTPPRRARPVIAIDGPAGAGKSTVAKAVADRLGFIYIDT
                     GAMYRAVALLAVQHGTGIHEPERLAALAESARFEFRRDARGRSRLHLNGADVEDDIRT
                     PEIGRLSSPVSEVAGVRSALVPLQRAMGAGGGVVMEGRDIQTNVFPDAEVKVFLTATE
                     P"
BASE COUNT          389 a          933 c          992 g          465 t
ORIGIN      
        1 tcgggccggt caggggcccc gcgccctctg ggcgcacccc tgtgcggccc ctccgccaga
       61 cccggacgtg ccgtctcggt gctcctgtgg tctcatctcg ggcctgactt ggcgccaagg
      121 gggcctggcc cctgtgccgc catttgccgc tccgatgcgc ctggtgtaca cttcgagccc
      181 actggcagcg gcagccgtgg atgcccccga gggcgttcgc gccctccgct ggaagggacc
      241 atcgaccgtg tccgaggacg cccggagact cagcgagatt tgcccgctcg ttcgcccctg
      301 cctggaccgc ctcgaagcct accggcccgg gaagcccatc gaggaggtgc gccgggagct
      361 cgggctgtcc gaggtctata agctcgcctc caacgagaac cctctggggc cctctcggga
      421 ggccatcgag accatgcagc gggttctggc cgacgcctgg ctctacccgg aggacacgtg
      481 tttcgacctg gttcgcgagc tgtccgccca cctgggcgtt cctgcggaac ggctcatcat
      541 cggccggggc tccgatgagc tgatccacct gattggcatg gctttcgtca acccggggga
      601 ggagctcatc atggccgatc ctccgttccc catgtacccc atcacggcca ctcagatgga
      661 tggctgggcg gttcgtgtgc cgttgaagga ctacacacac gaccttccgg ccatggcggc
      721 ggccatcaca ccgaagacca aggtgatctt catcgccaat ccccacaatc ccacggggac
      781 catcgtgcgg aagcctgagc tcgaggcgtt cctgagtcgg gtgccggatc acgcgctggt
      841 ggtgatggac gaggcgtact gcgagttcgt ggatgatccg ggctacccca cctcgctgga
      901 ctacgtggcg gaggggcgga acgtggtggt gctgcggacg ttcagcaaga tctacgccct
      961 ggcgggcctg cgggtgggct acggagtgtt ccccgaccac ctggcgccgg cggtgaagcg
     1021 ggtgcggcct ccgttcaacg tgagcacagt ggctcaggcg gccgccgtgg cgagcctgcg
     1081 cgacgccggg caggtggcgc ggtcgaaggc gtacaacatc gaggcgcgcc ggtatctgtg
     1141 cggcgagttc gaccggctgg gtctggcgta tgcgccgagc caggcgaact tcatcctcgt
     1201 ggacctgggc gtcgactgcg tcgaggcgtt ccagcggctg ctccgcaggg gctacatcgt
     1261 gcgcaccggt gacgtgttcg acctgcccac ccatgtccga gtcagcttcg gcacacctga
     1321 gcagaacgcg gggttcatcg cggctctgga gcaggtgctg gccgaactcc gttaggatgg
     1381 cacccaaatg gccgagtgct tcgagcgcgt ggcaatactg ggggtcggcc tgatcggcgg
     1441 gtccatgggc atggcgctgc attcggccgg ggccgccggc atggtggtgg ggtgctcgcg
     1501 caccgagtca cgcctgtgcg aggcgaagcg ccggggcgcc atcgactcct acaccacgga
     1561 cccgctggcc gcggtggacg gggccgacct ggtcattctc tgcttgcccg tggcgtccat
     1621 tccggcctcg gcgcgcacga tcgcgccggt cctcagatcg gatgccattg tcaccgatgt
     1681 cggcagcgcc aaggcgtgca tcgtggacgc cctcgaagag ctgttcgacg gccggggcca
     1741 gttcgtgggc ggtcatccca tggcgggatc ggcccagcag ggcgtctggc acgcgcgtgc
     1801 caacctgttc catggagcca cctacttcgt tacgcccacc gggcggacgc cttgtcgggc
     1861 cacggacgcc gtcacctcac tggcgcatca gctgggcgcc cggccggtgc accttccgcc
     1921 ggtggaccac gaccgcgtgg tggcggcgat cagtcacttg ccccatgtgg tggcgtccgg
     1981 cctggcgtca cttgtgcccg agctgtgtgg tgcgttcgcc gacgtggctc tggaggcggc
     2041 ggccggcagc ttcgccgacg gcacgcgggt tgccgggagc gatccttccc tgtgggccga
     2101 gatcctggtg cagaaccggg ccgaggtgct gcgcgcactg gcgagtttcc gcgcgaggct
     2161 ggacgcgttc gagtccgccc tctccgcggg agacgaagcg gccacccggg agctgttcgg
     2221 cctcggcacc gaggtgcggc gtcggttcga ggccagagag gcacggcccg aaccgcccgg
     2281 acaggagggc tgactgtggg atccacctcg aagacgcctc cgcgccgtgc ccggcctgtc
     2341 atcgcgatcg atgggccagc gggagccggg aagagcacgg tggccaaggc cgtggccgat
     2401 cgtctcgggt tcatctacat cgacacgggc gccatgtacc gggccgtggc gctgctggcg
     2461 gtccagcatg gcacgggcat ccatgagccc gagcgcctgg cggccctcgc cgaatcggct
     2521 cggttcgagt tccgccgcga tgccaggggc cggagccggc tgcacctgaa tggcgccgat
     2581 gtggaggatg atatccgcac acccgagatc ggtcggctct cgagccccgt ctcggaggtc
     2641 gcgggtgtcc ggagcgccct ggttccccta cagagggcca tgggggcggg tggcggcgtc
     2701 gtgatggaag gccgggacat ccagacgaac gtgttccccg atgccgaggt caaggtcttc
     2761 cttacggcca ccgagccgg
//
LOCUS       DAOSPL010000525         2775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_36369_length_2775_cov_2.962132, whole genome shotgun sequence.
ACCESSION   DAOSPL010000525 DAOSPL010000000
VERSION     DAOSPL010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36369_length_2775_cov_2.962132"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..657
                     /locus_tag="PLQ54_10210"
     CDS             73..657
                     /locus_tag="PLQ54_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93676.1"
                     /translation="MYRRLALGLTVVIVAASCQTGGTVEEATGDRVVDRSPGEPTGRP
                     AGTSITEPSYAVRRVPQPGGPSVPLSMSVTAAPGRNIIRLPLGDFSLQLTQPWARENA
                     LRGGSSTMVETLRGSDLGLSLQYPLSRSTTVGLGLRSLFDPANDVRAGGMVSLGVRHD
                     MGGLSVGAGVQAYGELGASGRARSRFGSAFTVEF"
     gene            800..>2775
                     /gene="secA"
                     /locus_tag="PLQ54_10215"
     CDS             800..>2775
                     /gene="secA"
                     /locus_tag="PLQ54_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019550953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOS93677.1"
                     /translation="MFNPINWLDPSGRLVRKYSRVAQRINELEPAMKARDDDSLRQYV
                     MAIRDQVQNGRSIDEALPEVFAAVREASVRTLGQRHFDVQLIGGMVLHSGRIAEMKTG
                     EGKTLVATLPLTLNALLGKGAHLVTTNDYLAKRDAEWMGPIYRFFGLTVGIIQHDMDA
                     PDRLAAYASDITYGTNNELGFDYLRDNMAFSRDRLVQRGLYYAIVDEVDSILIDEART
                     PLIISGVKQASSDLYRRFDRIVASLTRDKHYEVEEKQHQVSLTDEGVEAVERALGIDN
                     LSDPECVEYVHHVNCALKAHTLYKRDVDYVVRDGQVIIVDEFTGRLMFGRRYGEGLHQ
                     AIEAKENVKVEQESQTLATITFQNFFRLYERLAGMTGTAKTEEPEFIRIYDMPVVIVP
                     TNRPVRRVDHPDIVYKTEEAKFRGIATEILQCHLRGQPVLVGTRSIEISERLSDRLSP
                     DRLQRLAMATLLVERLQTGGRGGDPKERRALEEQLFQPLDNLNPGAIRRAARMVDMPS
                     DPLSDDVVAAFAQAIGVPSEAERLAEVLRSGIPHNVLNAKYHEREAQIIAQAGRIGAV
                     TIATNMAGRGVDIQLGGKPPEGMDVSPDYETVRALGGLHILGTERHESRRIDNQLRGR
                     SGRQGDPGSSRFFVCLEDELWRLFGAQERFQSLQR"
BASE COUNT          475 a          870 c          925 g          505 t
ORIGIN      
        1 ctgccatggt gctgctatca tcaaggtagt tgaggtgtgt ctgttccaag tctgggttcg
       61 gggtgctgtg ggatgtaccg tcgtctggca ttgggattga ctgtggtcat tgtggctgcg
      121 tcatgccaga ccggcgggac ggtcgaagag gccacgggcg accgggttgt cgatcgctca
      181 ccgggagaac cgaccggccg gccggccggc acctcgatca ccgagccgtc gtatgcggtt
      241 cggcgcgtgc cccagcccgg cggtcccagt gtgcctctga gcatgtcggt caccgccgcg
      301 cccgggcgga acatcatccg gcttccgctg ggtgatttca gcctccagct tacgcagccc
      361 tgggccaggg agaacgccct gcgaggcggc tcgagcacga tggtcgagac tcttcgcggc
      421 tcagacctag ggctgagcct tcagtaccca ctttcgcgca gtaccacggt tggcctggga
      481 cttcggtcgc tctttgatcc tgcgaacgac gtccgggctg gcgggatggt ctccctaggc
      541 gtccggcacg atatgggcgg cctatcggtc ggtgccggcg tgcaggccta cggcgagttg
      601 ggcgcctcgg ggcgcgcgcg ctccagattc gggagcgcct tcaccgtcga gttctgaccg
      661 cacgaggtcc attccgctct ccgaacctcc tcggttctgc tttgacccat cacgtcgtac
      721 tcgggtgccg gatgcggatc gggtatccgc ttctcgcacg cgctgtgtta cgagtacggc
      781 cctccgatag ggggatctga tgttcaaccc gatcaactgg ctcgatccca gtgggcgtct
      841 ggtgcggaag tacagtcggg tcgcccagcg catcaatgag ctcgagccag ccatgaaggc
      901 ccgggacgac gactctcttc gccagtacgt gatggcgatt cgggaccagg tgcagaacgg
      961 ccgatcgatc gacgaggcgc tgccggaggt ctttgcagcg gtgcgcgagg cgtcggtgcg
     1021 caccctggga cagcgccact tcgatgtcca gctcatcggc ggcatggtac tccacagtgg
     1081 gcgcatcgcc gagatgaaga ccggggaggg caagactctc gtagccacgc tcccgctcac
     1141 cctcaatgcg ctcctgggca aaggggccca cctcgttacc accaacgact acctggccaa
     1201 gcgcgatgcc gagtggatgg gtccgatcta ccgcttcttt ggtctgacgg tcgggattat
     1261 ccagcatgac atggatgcgc cggaccgtct tgcggcctat gcttctgaca tcacctatgg
     1321 gacgaacaat gagctcggct tcgactatct tcgcgacaac atggcgttct cgcgcgaccg
     1381 cctggttcag cgcggcctct actacgccat cgtggacgag gtcgacagca tcctgatcga
     1441 cgaggcccgc accccgctga tcatctcggg ggtgaagcag gcctcgtcgg atctctaccg
     1501 gcggttcgac cggattgtcg ccagcctcac ccgcgacaag cactatgagg tcgaggagaa
     1561 gcagcatcag gtctcgctca ccgatgaggg cgtcgaggcc gtcgaacgcg cgttggggat
     1621 cgacaacctg tccgatcccg agtgcgtgga gtacgtccat catgtgaact gcgcactgaa
     1681 ggcccacacg ctgtacaagc gcgacgtgga ctacgtggtc cgcgatggcc aggtcatcat
     1741 cgtggacgag ttcactgggc ggctcatgtt cggccggcgt tatggcgagg gactccacca
     1801 ggccatcgag gccaaggaga acgtgaaggt cgagcaagag agtcagactc tggcgaccat
     1861 caccttccag aacttcttcc gtctctacga gcgcctggcg ggcatgacgg gcaccgcgaa
     1921 gaccgaggag cctgagttca tacgcatcta cgatatgccc gtggtcattg tccccaccaa
     1981 ccgccccgtg cggcgcgtcg atcaccccga catcgtctac aagacagagg aagccaagtt
     2041 ccgtggtatc gccacggaga tcctgcagtg ccacttgcgc gggcagccgg tgctggtcgg
     2101 cacgcggtcg atcgagatct cggaacggct cagcgaccgg ttgagccctg atcggctgca
     2161 gcgcttggcc atggccacgc tgctggtcga gcggctccag acgggcgggc gcggtggcga
     2221 ccccaaggag cgtcgcgcgc tggaggagca gctcttccag ccactggaca acctgaaccc
     2281 gggcgccatt cgtcgagccg cgcgtatggt ggacatgccg tccgacccgc tgagtgacga
     2341 cgttgtggcg gcgttcgccc aggccattgg ggtgccatcg gaggccgaac ggctggctga
     2401 ggttctacgc agtggcatcc cgcacaacgt cctcaacgcc aagtaccacg agcgagaggc
     2461 ccagatcatt gcgcaggcgg ggcgcattgg cgccgtgacc attgcgacga acatggcggg
     2521 ccgcggggtg gacatccagc tcggcggcaa gcctcccgag ggcatggatg tgagccccga
     2581 ctatgagacc gtaagggcgt tgggcgggct gcacatcctg ggcaccgagc ggcatgagag
     2641 tcggcgtatc gataaccagc tccggggccg gtcgggtcga cagggcgacc cgggctcgtc
     2701 gcggttcttc gtgtgtctgg aggacgagct ttggcgcctg ttcggcgcac aggaacgatt
     2761 ccagagcttg cagcg
//
LOCUS       DAOSPL010000526         2771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_36439_length_2771_cov_3.446981, whole genome shotgun sequence.
ACCESSION   DAOSPL010000526 DAOSPL010000000
VERSION     DAOSPL010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36439_length_2771_cov_3.446981"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="PLQ54_10220"
     CDS             complement(<1..1271)
                     /locus_tag="PLQ54_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS93678.1"
                     /translation="MRAEGRLSEWAHALRLPLMGAAICAVFAVLVPGQFANGTNAGNI
                     IRATTTDTLAAIGLTLVMLVGHLDLSIGATMCLGGIVLIFTQPLLGWPGAVLAAGVAG
                     SVVGLANGLLVSRAKVNSFIATLGTMTILQGVCRALLEGGSKSLPDAVVGLQAAEWLS
                     PVLPWSPRVLLVVAPLALTEVVLRRTAFGRNLYLVGADSQVAFNAGVPVTRLLTLAFV
                     ASGILSALGGALTTAAQNTVMPNLGEKSLMLVIAAVIAGGTAMSGGRGTVSMSVLALL
                     LLNTLTNGMSYLGASKSAKLVAQGAVLAAIILHDAWRQARLDRVRGQRRQLLRELETQ
                     GLPLASVEDTISAQGSPDMQPRQHDRIFAMVCVTGMACVAMVTIYAMWSRGRASAPPT
                     LASIAGAPEAPGSATQTDTAGALSLKATDGQ"
     gene            complement(1261..2265)
                     /locus_tag="PLQ54_10225"
     CDS             complement(1261..2265)
                     /locus_tag="PLQ54_10225"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS93679.1"
                     /translation="MTQPGLTPSSGPGLRVHRWLGLWGVYLVVALLLAIGRTLSADFL
                     SADSLLQVVRDVSILGIVAVGVSLVTVSGHYVDLSIPAIMAVSGIVAVALLPAGLATA
                     LIAALAVGVLLGLINGTVIGYLRLNPIIWTLAALAVFDGTTRWAYGGKWVYVDKNTAT
                     GALFSQVYRGSVLGVVPVAVLLLAVSAGLSYVLLQHTVFGKRLALTGAAYDAARLTGV
                     PVRRVVMLAFVWSGLMAAVAGILKTSLNMYGDVEIGATYDFQAITAVVLGGVALAGGR
                     GTVAGVIGGVLAIGLLGRILPLIPGVGQDQQLAIRGMLFVIIVALSSYFMRKAGHRES
                     "
     gene            complement(2262..>2771)
                     /locus_tag="PLQ54_10230"
     CDS             complement(2262..>2771)
                     /locus_tag="PLQ54_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS93680.1"
                     /translation="RKTQGLALGLPVEENIVAAIWGRLSRRGVYRRTEGRAVASRWVD
                     ALHIQPPDLRREAGQLSGGNQQKVLLAKWLATGPRVLVLDEPTRGVDVGAKAAIHRAI
                     ADLARQGRAIVLVSSDLPELASLSHRAIVLREGRVIGELRGDALTPERILLAASGQPP
                     PAMDEEGAA"
BASE COUNT          481 a          926 c          983 g          381 t
ORIGIN      
        1 tgctggccgt cggtagcttt caggcttagg gcacccgcgg tatctgtctg ggtggcgctg
       61 cccggcgcct ccggcgcccc cgcgatcgag gccaatgtgg ggggcgccga ggcgcgcccg
      121 cgactccaca tggcgtagat cgtcaccatg gccacacacg ccatcccggt aacgcacacc
      181 atggcgaaga tgcggtcgtg ctgtcgtggc tgcatgtccg gactcccttg cgcggagatg
      241 gtgtcctcga cggaagccag gggcagtccc tgcgtttcca gctcccgaag cagctgtcgc
      301 ctctggccgc ggactcgatc gaggcgcgct tgtcgccagg cgtcgtgcag gatgatggcg
      361 gcaagcaccg ctccctgggc caccagtttg gcggacttgg atgcgccgag gtagctcatg
      421 ccgttcgtga gcgtgttgag caagagcaag gccagaacac tcatgctgac agtgccccgg
      481 ccgccgctca tggcggttcc gcccgcgatc acggcggcga tgacgagcat gagcgacttc
      541 tcgccgaggt tcggcataac ggtgttctgc gcggcggtgg tcagggctcc cccgagagca
      601 gagaggatgc ccgatgccac gaaggccaga gtcagcagac gggtgacggg cacgccggca
      661 ttgaaggcga cctgcgagtc ggcgcccacg aggtacaggt tccggccgaa ggccgtgcgg
      721 cgcagcacga cctcggtcag ggctagaggc gccacgacga gcagcacccg cggcgaccac
      781 gggagaaccg ggctgagcca ctcggccgcc tgcaggccga ccaccgcgtc gggcaaggac
      841 ttgctgccac cctccagcag cgcccggcac acgccttgca ggatggtcat ggtgccgagc
      901 gtcgcgatga acgagttcac cttggcccgc gaaacgagca ggccgttggc cagaccgacg
      961 acggagccag ccaccccggc cgccaacacg gcgccgggcc agccgagcaa cggctgggtg
     1021 aagataagta caataccacc gaggcacatg gtggccccga tggagaggtc caggtgtccg
     1081 accagcatga cgagggtcag accgatggcg gccagtgtgt ccgtggtggt cgcgcggatg
     1141 atgttgcccg cgtttgtccc gttggcgaac tgccccggca cgaggacggc gaacacggca
     1201 cagatcgccg ctcccatgag gggcaaacgg agggcgtgag cccactcgga gaggcggcct
     1261 tcagctctca cggtgccccg ccttacgcat gaagtaggag ctcagcgcca cgatgatgac
     1321 gaacagcatc ccgcggatgg ccagctgctg atcctgcccg acaccgggga tgagcgggag
     1381 gatcctcccc aggaggccga tggccagcac gccgccgatg acgccggcca cggtgccccg
     1441 tccgccggcc agcgccacgc cgcccaggac cacggcggtg atggcctgga agtcgtaggt
     1501 ggcgccgatc tcgacgtccc cgtacatgtt caggctcgtc ttcaggatgc ccgccacggc
     1561 agccatgagg cccgaccaga cgaaggccag catgaccacg cgccggaccg gcacccccgt
     1621 gagccgggcg gcatcgtagg cggccccggt gagggcgagg cgtttgccga acacggtgtg
     1681 ttgcagcagc acgtagctca agcccgccga cactgcgagg agaaggacgg ccacaggcac
     1741 gacgccgagt acgctgcccc gatagacctg ggagaacagg gcgccagtgg ctgtgttctt
     1801 gtcgacatag acccacttgc cgccgtacgc ccatcgtgtg gtgccgtcga agacggccag
     1861 ggcagccagg gtccagatga tcgggttcag gcggaggtag ccgatcaccg tgccgttgat
     1921 gaggcccagc aacacgccca cggcgagagc ggcgatcaat gccgtggcca gcccagctgg
     1981 gagcagcgcg acggcgacga tgcccgacac ggccatgatg gcggggatcg acagatcgac
     2041 atagtgtccg ctgactgtga cgagactcac accgacggcc acgatgccga ggatggacac
     2101 gtcgcggacc acctgcagca ggctgtcggc cgagaggaag tcggccgata gcgtccggcc
     2161 gatcgccagc aggagggcaa cgacgagata cacgccccag aggccgagcc agcggtggac
     2221 ccgcaggccg ggcccagagg agggcgtgag tccgggttgc gtcatgccgc cccctcctcg
     2281 tccatcgccg gaggcggttg cccgctcgct gccaggagga tgcgctcggg tgtcagagcg
     2341 tcgccacgga gctcaccgat gactcgtccc tcacggagca cgatggcgcg gtgcgagagg
     2401 ctggcgagct cgggcagatc gctggacacg agcacaatgg cgcgtccctg gcgggccagg
     2461 tcagctatcg cgcgatggat ggccgccttg gcaccgacgt cgacccctcg cgtcggctcg
     2521 tcgagcacca ggactctcgg gccggtggcg agccatttgg cgaggagtac cttctgctgg
     2581 ttcccgcccg acagctggcc ggcttcgcgg cgtaggtcgg gtggctgtat gtgcagggcg
     2641 tccacccatc gcgacgcgac ggcccggccc tcggtccggc ggtacacgcc ccggcggctg
     2701 aggcggcccc agatggcggc cacgatgttc tcctcgaccg ggagcccgag ggcgaggccc
     2761 tgggtcttgc g
//
LOCUS       DAOSPL010000527         2769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_36474_length_2769_cov_3.942520, whole genome shotgun sequence.
ACCESSION   DAOSPL010000527 DAOSPL010000000
VERSION     DAOSPL010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36474_length_2769_cov_3.942520"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..1457)
                     /locus_tag="PLQ54_10235"
     CDS             complement(183..1457)
                     /locus_tag="PLQ54_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93681.1"
                     /translation="MDRDTGSPLVYLEPDPEYSLLDSAADSVRFAVSRCLTEYRGHAC
                     ATSSFVNPEGEVMAWHDFGLIEGPGWAANAMGGAYELLLWARHSADERLRAKALSLID
                     HVLDDGFIDWETGLMHGYRKVDTDQRLLNYEKTNEWLCAGSMARVGCQMLWAADLLGE
                     DPRAARLVRAAQGMMGWIGERVAPLDNGWYPRRVRPDGRPYREDDPIFDHSGDGLFVV
                     ALMADLTQRGLADHRAELARRAEAFVRAGGMYGSINHDTYDSHECVAYAVGFRTLLQC
                     ARLLGREDLKAFAFDNALTGLRGFQMARDLNGVATKGLLYMEASWDTAYLWENAEAAL
                     AFVEAAEALETVAPRQAERCRRTALTILRAIAKHHYGPYGFLTEGVDWNNHVGQQHHI
                     DGAEFGAIQYTEPFLNNLHIVEPTLRLCGTEG"
     gene            complement(1557..>2769)
                     /gene="melA"
                     /locus_tag="PLQ54_10240"
     CDS             complement(1557..>2769)
                     /gene="melA"
                     /locus_tag="PLQ54_10240"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOS93682.1"
                     /translation="AYLDGRMPPRCINPEAAEGVGRFVQRVIDDNELPARVEVATDRR
                     EVLAGADYVIVSIAVGGPAYDGVPYYHEVAIPAEYGVEQQIADTLGPGGVFRALRCAP
                     EMLAICDDMEELCPEALLINYTNPMAMLCWAMGAATELDIVGLCHSVQGTAHQLAGYI
                     GVPADEVTYWVAGINHMSWFLEFAHQGRDAYPLLFEAMEKPEVFKQDPVRFEIMRHFG
                     AFVTESTPHMSEYVPYFRKRPELIEEFGLHRRRPNKEAEKRERFWQKDDPKESTELHR
                     SGEYASCIIHAIETNTPYRFNGNVPNSGLITNLMEGCCVEVPCLTDGTGVRPCGVGDL
                     PPQLAALNSSNIAVQQLTVEAVLERDLEKAYMAVALDPLTAAVCSLREIRSMFDAMVE
                     AEKPWLEAWYG"
BASE COUNT          448 a          994 c          878 g          449 t
ORIGIN      
        1 ggatgtccgg ccaaaactca tccagatgcc cgctgagccc gtcagcctgg atgcgcaact
       61 ggcgcccaag ccatccccgc gcgcggatgc ggccgagtgg cagtgggcgg aaggcaagcg
      121 gttggagttc gaacggagcc atgggcaact ctccttcggg ctgacggtgg gcgcaccatc
      181 ggctacccct ccgtgccgca cagccgtagc gtgggctcca cgatgtgcag attgttcagg
      241 aacggctccg tgtactggat cgcgccgaac tcggcgccgt cgatatggtg ctgctggccg
      301 acgtggttgt tccagtcgac accctccgtc aggaacccgt acggtccata gtggtgcttg
      361 gcgatggccc gcagaatcgt cagcgccgtc cgtcggcagc gctccgcctg acgcggcgcc
      421 accgtctcga gcgcctcggc cgcctcgacg aatgccagcg ccgcttcggc gttctcccag
      481 agataggccg tgtcccacga ggcctccatg tagagcaggc ccttcgtcgc cacaccgttc
      541 aggtcgcggg ccatctgaaa gccgcggagt ccggtgagcg cattgtcgaa agcaaacgcc
      601 ttcaggtcct ctcgccccag caggcgcgca cactgcagca gcgtgcggaa acccaccgca
      661 taggccacac actcgtgcga atcgtaggtg tcgtgattga tgctgccgta catgccgccg
      721 gcgcggacga acgcctcggc ccggcgcgcc agctccgccc ggtgatcagc aagcccccgc
      781 tgcgtgaggt cagccatgag cgcgacgacg aacagcccgt cgccgctgtg gtcgaagatg
      841 gggtcatcct cgcgataggg ccgcccgtcg ggccgcacgc ggcgcgggta ccagccgttg
      901 tccagcggtg ccacacgctc accgatccag cccatcatcc cctgcgcggc gcgcaccagc
      961 cgcgcggcac gcgggtcctc gccgagcaga tcggccgccc agagcatctg gcagcccacg
     1021 cgagccatcg acccggcgca cagccactcg ttcgtcttct cgtagttgag cagccgctgg
     1081 tcagtgtcga ccttacggta gccgtgcatc aggccggttt cccagtcgat gaacccgtcg
     1141 tcgagtacgt ggtcgatcag cgacagcgcc ttggcccgga gccgctcgtc agcgctgtgc
     1201 cgggcccaga gcaggagctc gtaggcgccc cccatggcgt tggccgccca cccggggccc
     1261 tcgatgaggc caaagtcgtg ccacgccatc acctcgccct cggggttaac gaaggacgaa
     1321 gttgcgcacg catgcccgcg gtactctgtc aggcaccggc tcaccgcgaa ccgcaccgag
     1381 tcggcggcgc tgtcgagcag cgagtactcc ggatccggtt cgaggtagac cagcggcgac
     1441 ccggtgtccc gatccatggc agcgtgctcc ctcgcggtac atctctcgtc ccaggggcac
     1501 cggcctcctc gccggtgagt cggccgccag gccgacggct ggctcgcgtc cctgccctac
     1561 ccgtaccacg cctcaagcca cggcttctcc gcctcgacca tggcgtcgaa catcgaccgg
     1621 atctcacgaa gcgagcacac ggccgccgtg agcggatcca gcgccacggc catgtaggcc
     1681 ttctccagat cccgctcgag cacggcttcg accgtaagct gctgtacggc gatgttcgac
     1741 gagttcagcg cggcgagctg cggcgggagg tcgccgacgc cgcacggccg gacgcccgtg
     1801 ccgtccgtca ggcacggcac ctcgacgcag cacccctcca tcaggttcgt gatcagcccg
     1861 ctgttcggca cgttgccgtt gaaccgatag ggcgtgttcg tctcgatggc gtggatgatg
     1921 catgaggcgt attcgcccga acgatgcagc tcggtagact ccttggggtc atccttctgc
     1981 cagaagcgct cacgcttttc ggcctctttg ttcggccggc ggcggtggag cccgaactcc
     2041 tcgatcagct ccgggcgctt gcggaagtag ggcacgtact ccgacatgtg cggcgtcgat
     2101 tcggtcacaa atgcgccgaa gtggcgcatg atctcaaacc gcaccgggtc ctgcttgaac
     2161 acctcgggct tctccatggc ctcgaacagc agcgggtacg catcgcgtcc ctgatgcgcg
     2221 aactcgagga accacgacat gtggttgatc cccgcgaccc agtaggtcac ctcatcggcg
     2281 ggcaccccga tgtagcccgc gagctgatgc gccgttccct ggacgctatg gcacaggccg
     2341 acgatatcga gctcggtggc cgcgcccatg gcccagcaca gcatggccat ggggttcgtg
     2401 tagttgatca gcagcgcctc agggcacagc tcttccatgt cgtcgcagat ggccagcatc
     2461 tccggcgcgc acctcagcgc gcggaacacg ccgcccggcc ccaacgtatc ggcaatctgc
     2521 tgctcgacac catactcggc cgggatcgcg acctcgtggt agtagggcac gccgtcgtag
     2581 gccgggccgc cgacggcgat ggacacgatg acgtagtcgg cccccgccag cacctcgcgg
     2641 cgatccgtcg ccacctcaac gcgcgcgggt agctcgttgt cgtcgatcac ccgctggacg
     2701 aaccgcccca cgccctccgc cgcctccggg ttgatgcagc gggggggcat ccgcccatcc
     2761 agataggcg
//
LOCUS       DAOSPL010000528         2766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_36527_length_2766_cov_4.290668, whole genome shotgun sequence.
ACCESSION   DAOSPL010000528 DAOSPL010000000
VERSION     DAOSPL010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36527_length_2766_cov_4.290668"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2766)
                     /locus_tag="PLQ54_10245"
     CDS             complement(<1..>2766)
                     /locus_tag="PLQ54_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93683.1"
                     /translation="LPSLDEFSKPPTEPGRTEAVLETPIQLDGRAVRRVIVADATWPG
                     MSHLPADTAVALPVSTPVYVSGLTDRGFQALSAALQTYNIQAIVGPASADPDRKIDLQ
                     PGSACSLTLVRGDMDMSAGGTLTYRQGDRILAFGHSMMGFGPTDLPLAGAEVNEFVPG
                     INIGFKVMSPYATVGTIYTDTNWAVGGILGRESASIRFDVEVANESTGQSRTFHLELC
                     DNSVLTLPLLLSAASSAMETVTCIGQEATADVTTEVVSDRGTLSRHNVCYDQRDVTSA
                     ALSDLIDGALVMTRNVFDKARVKSAQLRCVIRHGRTQATLENLEIDDYVVRPGETISV
                     GVVLRLDKGERVRRTLELVIPHDTPTCQLQLGVTAGALRTAVESRLGIVTPDPRQLGD
                     LLELYQRVPPQNRLVALLPLQSRSIITKGVQLHDIPVLMGAVVGTAGVTDLAEGVAHI
                     EATEDTPWDVQGALFHPVIVIRPEDTPTEEVLKAGESRGRVGGQGAPSQVISAADTQL
                     QMSAYVRVEPALRQLLADLGLTGRLTSLGGPALDQTPAQFLGDSLRPSMLRSLSARPG
                     APAQRPRGIPDLPGLPDAPDLPDIPEGAVTKVAEGEAEGEATKEASEAKASEEGKKEK
                     EDTKIEAVTVRGPETWTQATREDFGRGKLSGLAVSDRGEVTLSAPLSVFCPSLPALYP
                     WDCALGDSGELLVATGPEGAILRVDTTGRAAPFFTVDDTMVFSLVRAADGTIYAGTGP
                     KGLIYRISPDGAGEVVCHTGERYVFDLLLTRDGELLAATGPHGRILRVSGDEPQVIYE
                     GPERHVLVLCQGEDGTIYAGTGDKGSVLRIHEGGATVVCQSPMGAVHALVAGKDGAVY
                     FGGGSTDSTAIFRVSAKGDVERLYGNDDGALLAMATDDDGHVYAGTSVFARLLVADSA
                     GHVALLG"
BASE COUNT          454 a          946 c          925 g          441 t
ORIGIN      
        1 cgccgagcag cgccacatgc ccggctgagt cggctaccag gagccgtgcg aacacactgg
       61 tcccggcgta cacgtggcca tcgtcatcgg ttgccatggc gaggagcgcg ccgtcatcgt
      121 ttccatatag acgttcgaca tcccccttgg cagagacgcg gaagatcgct gtcgagtccg
      181 tggagccgcc gccgaagtag acggcaccgt ccttaccggc caccagggcg tgcacagccc
      241 ccatgggtga ctggcagacc acggtggccc cgccttcatg gatgcggagt accgacccct
      301 tgtcgcccgt gcccgcatag atggtgccgt cctcgccctg gcacagaaca agcacgtgcc
      361 gctccggacc ctcgtagata acctggggct catcgccgct gacccgcaga atccggccgt
      421 gggggcccgt ggcggccagc agctccccgt cccgggtcag cagcaggtca aagacgtacc
      481 tctcgcccgt gtggcaaacc acctcgcccg cgccatcagg ggagatccgg tagattagtc
      541 ccttgggacc ggtgcccgcg tagatcgtgc catcggcagc ccgcaccagg ctgaagacca
      601 tggtgtcatc cacggtgaag aagggtgccg cgcgcccggt ggtgtccact cggagtatgg
      661 ccccctcggg ccctgtggcc acgagaagct ctccggaatc gccgagggcg cagtcccagg
      721 gatagagcgc cggcaagctg gggcagaaca cggagagtgg cgccgagagg gtgacctcgc
      781 cccggtcgct gacggccaaa cctgagagct tgccgcgccc gaagtcctct cgcgtggcct
      841 gggtccaggt ctctggcccg cgcacggtga cggcctcgat cttggtatcc tccttctcct
      901 tcttgccctc ctcggaggcc ttggcctcgc tggcttcctt cgtggcctcg ccctccgctt
      961 cgccctcagc gaccttggtc accgcgccct cggggatgtc gggcaggtcg ggagcgtccg
     1021 ggaggccggg caggtccggg atcccccgcg gccgctgcgc gggggcgcct ggccgcgcag
     1081 acagactgcg gagcatgctg gggcgcagac tatcacccaa gaactgcgcg ggtgtctgat
     1141 cgagtgcagg accgccgagc gacgtgagtc gccccgtcag gcccaagtcc gctaacagct
     1201 gccgaagagc gggctcgacg cgaacgtacg cagacatctg cagctgggta tcggccgccg
     1261 agatgacctg ggagggggcc ccttggccgc cgacgcgtcc ccgcgactcg ccggccttga
     1321 ggacctcctc ggtcggtgtg tcctccggcc ggatgacgat caccggatgg aacaacgccc
     1381 cttggacatc ccacggcgtg tcctcagtgg cctcgatatg cgcgacaccc tccgccaggt
     1441 cggtcacgcc ggccgtgccg acgacggcgc ccatcagcac agggatgtcg tggagctgga
     1501 cgcccttggt gatgatcgag cggctctgaa ggggcagcag ggcgaccaga cggttctgcg
     1561 gcggcacgcg ctggtacagc tccagaaggt cgccgagctg gcgcggatca ggcgtcacga
     1621 tgcccaggcg cgactccacc gccgtgcgca gcgcgccggc ggtcacgccc agctggagct
     1681 gacacgtagg cgtgtcgtgg gggatcacca gctccagggt ccgccggacg cgttcgccct
     1741 tgtcgagtct cagaaccacc ccgaccgaga tcgtctcgcc gggccgtacc acgtagtcgt
     1801 caatctcgag gttctccagt gtggcctggg tacggccgtg ccggatcaca catcgcagct
     1861 gggccgactt cacgcgtgcc ttgtcgaaca catttcgcgt catcaccagg gcgccgtcga
     1921 tgagatccga aagggcggcg ctggtgacgt cccgctggtc gtagcacacg ttgtggcgtg
     1981 agagcgtacc ccgatcggac accacctcag tcgtgacatc ggccgtagcc tcctgaccga
     2041 tgcaggtcac cgtctccatg gcagacgatg ccgccgagag caacaggggg agcgtcagca
     2101 cgctgttgtc acacagctcg aggtggaagg tgcgcgactg gcctgtgctc tcattggcaa
     2161 cctccacgtc gaaccggatc gacgcgctct cacggccgag gatcccaccg accgcccagt
     2221 tcgtgtcggt gtagatggtt cccaccgtgg cgtacgggga catcaccttg aacccgatgt
     2281 tgatgcccgg aacgaactcg ttgacctcgg ctcccgccag cggaagatcg gtggggccga
     2341 agcccatcat ggagtgaccg aaggccagga tccgatcccc ttggcggtag gtaagtgtgc
     2401 cgccggcgga catgtccatg tcgccgcgga ccagcgtgag cgagcaggca ctgccgggct
     2461 gtaggtcgat cttccgatcc gggtcggccg acgcgggccc cacgatcgcc tggatgttgt
     2521 aggtctgcaa ggcggccgag agcgcctgga aacctcggtc ggtgagccca ctaacataga
     2581 ccggagtcga cacaggcagc gccaccgcgg tgtccgccgg gagatggctc atccccggcc
     2641 atgtagcgtc ggccacgatg accctccgca ccgcgcgccc atcgagctgg atgggggtct
     2701 ccagaacggc ttcggtgcgg cccggctctg tagggggctt cgagaactcg tccagcgacg
     2761 ggagca
//
LOCUS       DAOSPL010000529         2765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_36549_length_2765_cov_3.449077, whole genome shotgun sequence.
ACCESSION   DAOSPL010000529 DAOSPL010000000
VERSION     DAOSPL010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36549_length_2765_cov_3.449077"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PLQ54_10250"
     CDS             complement(<1..922)
                     /locus_tag="PLQ54_10250"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR01208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HOS93684.1"
                     /translation="MKGIVLCAGAGTRLRPLTHTGAKHLIPVANKAILFYAIEALADA
                     GVTDLGIVVGATGWQISDAVGDGARFGCRVTYIPQERPLGLGHAVRVARDFVGDSRFV
                     VYLGDNFIHGGIGRFVSDFANARSAGQILLSPEADPSRYGVAQVDGDRIVRLVEKPKD
                     PPSNLCIVGVYAFGPEIFDSIERTPPSARGELEITDAIQGLIDAGLEVRKYEIESWWK
                     DTGKPEDVLDLNRRLLEELEPGVHGDINAESVIEGRVYVEKGATVRRSHLRGPVIIAE
                     GCEVEGSYIGPYTSIGPKTRIVNSDIEYSIL"
     gene            1075..>1594
                     /locus_tag="PLQ54_10255"
     CDS             1075..>1594
                     /locus_tag="PLQ54_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93685.1"
                     /translation="MGRAVAAALVASGTIGLITSGALAQTDAPLDDFERENGPSWSFS
                     NGPEFPGASGHFERSPDAAHSGAHGGRLYFDFSAGGAYVQATVGLPAEPPAGAVELFA
                     RKTVTNSITVRAVDQQGQTFQKTFNFPEGDWRHLRIRMDGWSHHWGGPNDGVFHGAAK
                     ALGLLVERGEATT"
     assembly_gap    1595..1604
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1605..>2765
                     /locus_tag="PLQ54_10260"
     CDS             <1605..>2765
                     /locus_tag="PLQ54_10260"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOS93686.1"
                     /translation="DFDDLTIVPAAHPAESARSIHEYVVARFGPEEGWGFWVSGPAGG
                     TQRDGGRVQYDFSLGASELHLGNDEALLGRPVSLRLRVRSDGSGHAVRMNLGSHFQSF
                     WRTLGTLDEPGEMVLEAPLGGMTEWEHGGGENDGVVRLPLRLLNLSLARREGGPERGD
                     IEFLELSVQTDVPPDRLLFPLARGPAAAGDPFRLTLRSLAAEPSDARVTCSLRDFSGR
                     VLRTEEHAMTLPASAQPVALQVPADVADVPFLEAEFRIQAPGQKPATAAACWVMPWPD
                     EGSAELDPASPWGMGLYLYRYGGDAAGLAQMDRAAALARAAGVKWTREEFQWHRIEPR
                     PGEFDWTFYDALVDTATRHGISVYGLIAYWSSWTQPYTDQGVEDYCRFVRALV"
BASE COUNT          420 a          925 c          942 g          468 t
ORIGIN      
        1 agaggatgct gtactcgatg tcggagttca cgatccgagt cttgggaccg atcgaggtat
       61 acggcccgat gtacgatccc tccacctcgc agccctcggc gatgatgacc ggcccgcgca
      121 gatggctgcg gcgcaccgtg gcccctttct cgacgtagac gcggccctcg atgacgctct
      181 cggcgttaat gtccccgtgg acgcccggtt cgagttcctc gagcaaccgg cggttgaggt
      241 cgagcacgtc ttcgggtttc ccggtgtcct tccaccagct ctcgatctcg tacttgcgga
      301 cctcgaggcc ggcgtcgatc agcccctgaa tggcgtcggt gatctccagc tcgccccgag
      361 cggagggggg agtccgctcg atgctgtcga agatctcagg accaaaggca taaacgccca
      421 cgatgcagag gttgctcgga gggtctttgg gtttctcgac gagccgcacg atgcggtcgc
      481 cgtcgacctg ggccacccca tagcgagagg gatcggcctc agggctgagc agaatctgcc
      541 cagcggagcg ggcattggcg aagtcggaca cgaaccgccc aatgccgccg tggatgaagt
      601 tgtcgccgag gtacaccacg aaccggctgt cgcctacgaa gtcccgcgcg acgcggacgg
      661 cgtgcccgag gcccagcggg cgctcctgtg ggatataggt cacgcggcag ccgaatcggg
      721 ccccgtcgcc cacggcatca ctgatctgcc agcccgtggc gccgacgacg atgcccagat
      781 cggtgacccc agcatcggcc agcgcctcga tggcatagaa gagtatggcc ttattggcca
      841 cggggatgag gtgcttggcg cccgtgtggg tcagcgggcg gagacgcgtg ccagccccgg
      901 cacatagcac gatgcccttc acagtggtgc ctccctgcgc ggcacggtcg gagcccgccg
      961 cgactgccat tacgttctac cacacttgct cgatacccgc tcacacggcg cgggcaggga
     1021 ggacccccgg ggggcacgaa aggtgccgtg cacgctgact tggggggatg agcgatgggt
     1081 cgggcggtcg ctgcggcgct cgtcgcgtcg ggcacgatcg ggctgatcac ctcgggggcc
     1141 ctggcccaaa ccgatgctcc attggatgac ttcgagcgtg agaacggacc gtcatggtca
     1201 ttcagcaacg gtcccgagtt tcctggggcg tccgggcact tcgagcgctc cccggacgcc
     1261 gcccactccg gggcgcacgg cggccgactc tacttcgact tctcggccgg gggtgcctat
     1321 gtgcaggcca ccgtcggcct ccccgcggag cctccggccg gggccgtcga gctgttcgca
     1381 cgcaagaccg tcaccaactc gatcaccgtc cgtgcggtcg accagcaagg ccagacgttc
     1441 cagaagacct tcaacttccc tgagggcgac tggcgccact tgcgcatccg catggatggc
     1501 tggagccacc actggggcgg acccaatgat ggcgtgttcc acggggccgc caaggcgctg
     1561 ggcctgctcg tcgagcgcgg ggaggcgacc accgnnnnnn nnnngatttc gacgatctga
     1621 ccatcgtgcc cgcggcccat cccgccgagt cggcgcgttc catccatgag tacgtggtcg
     1681 cgcgttttgg gcccgaggag ggatggggct tctgggtatc cggaccggca ggcggcaccc
     1741 agcgggacgg cggacgcgtc cagtatgact tctccctggg cgcctctgag ctccacttgg
     1801 gcaacgacga ggctcttctg ggccgacccg tgtcactgag gctgagagta cgcagcgatg
     1861 gttctggtca cgcggtacgg atgaacttgg gctcgcactt tcagagcttc tggcgcacgc
     1921 tcggcacgct cgacgaaccc ggagagatgg tgctcgaggc ccctctggga ggcatgaccg
     1981 aatgggagca tgggggtggg gagaatgacg gtgtcgtccg tcttccgctt cgcctgctga
     2041 acttgagcct ggcgcgccgc gaaggcgggc ccgagcgggg cgacatagag ttcctcgaac
     2101 tgagtgttca gaccgatgtc ccgccggacc gcctgctgtt ccctctggcc cgggggcctg
     2161 ccgccgccgg tgatcccttc cggctgaccc tgcgcagcct ggctgcagag ccttcggatg
     2221 cccgggtgac ctgctcgctg cgcgacttct ctggacgtgt cctcaggacg gaggagcacg
     2281 cgatgacgct gcccgcgtcg gcccagcccg tcgcgctgca agtccccgcc gatgtggccg
     2341 acgttccctt cctcgaggcc gagttccgca tccaggctcc gggtcagaag ccggccacgg
     2401 cggccgcgtg ctgggtcatg ccgtggccgg atgagggcag cgccgagctc gatcccgcgt
     2461 cgccgtgggg gatggggctc tacctctacc gctacggcgg cgacgccgcc gggctggctc
     2521 agatggaccg cgcggcggcc ctggcgcgag ccgcgggagt gaagtggacc cgcgaggagt
     2581 tccaatggca ccgcatcgag ccgcgccccg gcgagttcga ctggaccttc tacgacgcgc
     2641 tggtcgacac ggccactcgg cacggcatca gcgtctatgg tctcatcgcc tattggtcct
     2701 cctggaccca gccctacacc gatcaggggg tcgaggacta ctgccggttc gttcgtgccc
     2761 tggtc
//
LOCUS       DAOSPL010000530         2761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_36627_length_2761_cov_2.600887, whole genome shotgun sequence.
ACCESSION   DAOSPL010000530 DAOSPL010000000
VERSION     DAOSPL010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36627_length_2761_cov_2.600887"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="PLQ54_10265"
     CDS             <1..1035
                     /locus_tag="PLQ54_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93687.1"
                     /translation="VPEDQPAHWARATARSASNAGALVLSNPHGAGAGPPDQALGPAA
                     VSWQRAGLYGARLFEISPRSGRPPRLDDDLAFWDSCLAQGLGVVGIGVSGDSTGGESP
                     REGTFVTWVRSPEASLSAVLQSVRAGHAWFGDPLAANGPIEITASPGGFGAGSVVLTD
                     RDWCTLTLNAQGLPPDAELVWVAAGERVAAGRVPTGGGPFAAALDLRIPKRGTAVRAE
                     AYDREGLPLLFSNAITFLRELPAGTDALPEDRGGIALGEWVVHDVQRFAIAQLVPVEG
                     GVDIHGTGLGGGFAVDCGRKGPPASVRAVSEFGVARPLDYEWDASTVRIAGLTDRQVV
                     QIRWHGKPNR"
     gene            1011..>2761
                     /locus_tag="PLQ54_10270"
     CDS             1011..>2761
                     /locus_tag="PLQ54_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93688.1"
                     /translation="MARQAQSMTAREVPPPPWPPRPIAMALLVVACAFGAAPGHAQIP
                     SAVNLVRNGSMERDVDGDKRPDLWDLRSDAGGSGMLLEETPDGGHCLAVWHREEQPGY
                     TAVVTTVTRPLQAMAWYRVTLLARGADVTQLRIQLLDGRTGLVCGTTALIPNITPDWG
                     RRAAYVRTTDAASPGMLSIAFEGPGELRLDDVSLEELQPSDHNIWPAEDRPNLVYNGS
                     FELGGAGWATLGYGGLVGDIDRSTAVHGQASYRLRWSADALPMVVSDYPRPTVAPATD
                     LQLATLGWLRLQPGKPYSYSLYLKTDGAQLPVQIGMVFLDQSVARSGEVVMAGPTWCR
                     YRGTLTPPSDLAFLVAQPSAPPPDHAGPTLWVDSVVVAPADPPERYVSRYPVELALSP
                     VGGAWIGPAGQPLAVRVDVFNYDDAPFAGSVHVRTIDYFGDLVDQRDLPADVPPGAMG
                     SVNYELQGTPNGVFRVSASVKLAEGEPTQLARVARVPAYPMTADSAASPFGVSRGYPY
                     PERIQAGRMAGLLSSRTLALNWAQIEPEPGQADFSGADRLVDLLAGLGQDILACVPFP
                     SAPWANQATEEQIRSAGV"
BASE COUNT          375 a          998 c          945 g          443 t
ORIGIN      
        1 gtgcccgaag accagccagc ccactgggcc agggccaccg cgcggtctgc cagcaacgcc
       61 ggggccctgg tgctgtccaa tccccatggg gcgggcgccg gaccccccga tcaggcactc
      121 gggccggccg ccgtgtcctg gcagcgcgcc ggcttgtacg gtgcgcggct gttcgagatc
      181 agccctcggt ccggccgccc cccgcggctc gacgacgact tggcgttctg ggacagctgt
      241 ctcgctcagg ggttgggtgt ggtgggcatc ggggtctcgg gtgacagcac gggcggcgag
      301 agtccccgcg aggggacctt cgtcacttgg gtccgctcgc ccgaggcgag cctgtcagcc
      361 gtgctccagt ctgtccgagc cggccacgcc tggttcggtg accccctcgc cgccaacggc
      421 cccatcgaga tcaccgcgtc tccgggaggc tttggggccg gcagcgtcgt gcttaccgat
      481 cgggactggt gcaccctcac gctgaacgcc caagggctgc cacccgatgc ggagctggtc
      541 tgggtggcgg ccggcgagcg cgttgcggcg gggcgcgtcc cgactggcgg gggcccgttc
      601 gctgcggccc tcgacctgcg gatcccgaag cgcggcacgg cggtccgcgc cgaagcctat
      661 gatcgcgagg gcctgcccct gctcttcagc aacgccatca ccttcctccg agagcttccc
      721 gccggaaccg acgctctgcc cgaggaccgc ggcgggattg ccttgggcga gtgggttgtg
      781 catgatgttc aacggttcgc gatcgcccag ctggtgcccg tcgagggcgg agtggacatc
      841 cacggcaccg ggttgggcgg agggttcgcg gtcgactgtg gccggaaggg gcctccggct
      901 tccgtgcggg ccgtcagcga gttcggagtg gcgcgcccgt tggactatga gtgggatgcg
      961 agcacggtca ggatcgcggg cttgaccgac cgacaggtgg tgcagatccg atggcacggc
     1021 aagcccaatc gatgacggcg agggaggtgc ccccacctcc gtggccgccg cgccccatcg
     1081 ccatggctct gctcgtcgtg gcgtgcgcgt tcggtgccgc cccgggccac gctcagatcc
     1141 cgtccgcggt gaacctggta cgcaacggct ccatggaacg cgatgtggat ggcgacaagc
     1201 gccccgacct gtgggacctg cggtcggacg ccggcggctc gggcatgttg ctcgaggaga
     1261 cccccgacgg cggacactgc ctcgccgtct ggcaccgcga ggagcagccg ggctataccg
     1321 ccgtcgtgac cacggtaacc cggccactac aggccatggc ctggtaccgc gtgaccctcc
     1381 tcgcccgggg cgccgatgtg acccaactgc gcatccagtt gctggatggc cggacgggac
     1441 tcgtctgcgg caccacggcc ctgatcccga acatcacacc cgactggggt cgccgcgcgg
     1501 catacgtgcg aaccaccgat gctgcgtcgc ccggcatgct cagcatcgcc ttcgagggac
     1561 cgggtgagct tcgtctcgac gacgtgtctc tcgaggagct ccagccgtcc gaccacaaca
     1621 tctggcccgc cgaggatcgg ccgaatctgg tctacaacgg cagcttcgag ctgggagggg
     1681 cgggttgggc gaccctgggc tatggcggcc tggtcggcga catcgatcgc tcgacggcgg
     1741 tccacggtca ggccagctac cgccttcggt ggtctgccga cgcactaccc atggtggtgt
     1801 ccgactatcc caggccgacc gtcgcccccg ccaccgatct ccaactcgcg accctgggat
     1861 ggctccgcct tcagccgggt aagccctact cctactccct gtacctcaag accgatggcg
     1921 ctcagttgcc ggtgcagatc ggtatggtgt tcctcgatca gagtgtggcg cgctcgggtg
     1981 aggtggttat ggccggcccc acgtggtgtc gctaccgcgg cacactcacg ccgcccagcg
     2041 acctcgcgtt cctcgtggcc caacccagtg cgccgccgcc cgaccacgcc ggccccactc
     2101 tgtgggtgga tagcgtcgtc gtggccccgg ccgacccgcc cgaacgctac gtttcccggt
     2161 acccggtcga gctggccctc tccccggtgg gaggcgcatg gatcggcccc gccggccagc
     2221 cactggcggt gcgggtagat gtcttcaact acgacgacgc gccgttcgcc gggtcggtcc
     2281 atgtccggac catcgactac tttggtgatc tggtcgatca gcgggacctg cccgcagatg
     2341 tcccccccgg cgccatggga tcggtgaact acgaactcca ggggacgccc aacggcgtct
     2401 tccgcgtctc cgccagcgtg aagctcgcgg agggcgagcc cacccagctc gcgcgtgtgg
     2461 cgcgcgtgcc tgcctacccc atgacggccg attcggccgc atcccccttc ggagtgagcc
     2521 gcggctatcc ataccccgag cgaatccagg ccggccggat ggccgggctg ctctcgagcc
     2581 gaaccctcgc actcaactgg gctcagatcg agcccgagcc gggccaggcc gacttctcgg
     2641 gggctgaccg cctcgtcgac ctgctggccg gtctgggcca ggacatcctg gcgtgcgtcc
     2701 cgttcccctc ggccccctgg gccaaccagg ccaccgaaga gcagatccgt tccgctgggg
     2761 t
//
LOCUS       DAOSPL010000531         2758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_36689_length_2758_cov_2.384018, whole genome shotgun sequence.
ACCESSION   DAOSPL010000531 DAOSPL010000000
VERSION     DAOSPL010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36689_length_2758_cov_2.384018"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PLQ54_10275"
     CDS             complement(<1..490)
                     /locus_tag="PLQ54_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93689.1"
                     /translation="MRDTRLIAIITGAALLGLTVALVTPTFGAADTKVIYAGGPLAGQ
                     AVDKPGPWGSGSAALAEGGQWVTIKTQGYYQGGRFDIGQSVSLDELSQTPDNSYLVLE
                     VKVEPRAGDPPPVALPVTSDGTPMTDIPATVGTNRRMAVSMGAGPMMGAGPMMGGGGP
                     MMG"
     gene            complement(668..1810)
                     /gene="hemW"
                     /locus_tag="PLQ54_10280"
     CDS             complement(668..1810)
                     /gene="hemW"
                     /locus_tag="PLQ54_10280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOS93690.1"
                     /translation="MRPISVYIHIPFCRTKCLYCDFASVPANHDSVAAYMRALHTDAE
                     RQLASLASQGRSLATLYIGGGTPTVVPPADLAALLTVCLNSMPVEPDIEVTVEANPGT
                     VTRDTLARLRDAGANRLSLGVQSLSDDLLQALGRTHTATQAHQAITDAHAVGFSDLSA
                     DLIYGIPGQSPDRFEADLRELARRGLPHLSAYALTYEEGTPLRRARDAGRIAPMSEED
                     EAWCFARAGHVLAEYGLDRYEVSNFARPGAECRHNLTYWRRGEYLGLGAAAHSFIAGR
                     RWWNARQHDDYVRCVTDGVSYQEGLEEITPENAWTEAIMLGLRLREGIALDTLEGPPG
                     GTAEPPIWSKGYSLQQRGLLELADGRLRLTTKGLLVADAVTLELVS"
     gene            complement(1816..>2758)
                     /locus_tag="PLQ54_10285"
     CDS             complement(1816..>2758)
                     /locus_tag="PLQ54_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011887843.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HOS93691.1"
                     /translation="LPGYKVAQPVVFCGLYPTDNSEYDNLRDALEKLQLNDAALSYEK
                     ETSEALGFGFRAGFLGLLHMEIVQERLEREYGSNLIATAPNVVYRVHLTDGSAVDIDN
                     PSLFPTADRIVRVEEPYVLARISTPEEYVGPLMELCGDRRGVFSNMHYHDGQRVTLVY
                     ELPLAEILVDFFDQLKSRSRGYGSLDYEPIGVRPANVVRLDIWLNGDPVDALSVIIHR
                     DRAYERARDIVSRLKHVLPRQQFRIQILGAIGGRVIAAAAISPYRKNVLAKCYGGDIT
                     RKRKLLEKQKAGKKRMKMVGSVEIPQEAFMTLLKLDD"
BASE COUNT          428 a          846 c          996 g          488 t
ORIGIN      
        1 cgcccatcat ggggccaccg ccacccatca tggggccagc gcccatcatg gggccagcgc
       61 ccatggacac ggccatgcgc cgattcgtgc cgacggtcgc cgggatgtcg gtcatcgggg
      121 tgccatcgga ggtgactggc agcgcgacgg gcgggggatc gccggcccga ggctccactt
      181 tgacttcgag aacgaggtag gagttgtcgg gcgtctggct cagctcgtcc agcgacacac
      241 tctggccgat gtcgaacctc cccccctggt aatagccctg ggtcttgatg gtgacccact
      301 ggccgccttc ggccagagca gcggagccgc tgccccaagg tccgggcttg tcgacggcct
      361 ggccggccag cggtccgccg gcatagatca ctttggtgtc ggctgccccg aaggtgggcg
      421 tgactagcgc cacggtaagg ccgaggagcg ccgcaccggt gatgatcgct atcagcctgg
      481 tgtctctcat ggcgatctgc tgtcccctct cacacccggc cgaagacacc ggcgggtgtc
      541 acagtcccca atcggctcgt gggttcacgc cttcgggcgg ttcccacacc ttcagggcgt
      601 tggttcgcgc ggacccactc gtcatcctcc cgcgccgtgg cggcacggcc tggccgtgcg
      661 ccatgggtca cgacacgagc tcaagcgtca cggcatcggc gacgagcagc cccttagtcg
      721 tcaggcggag ccggccgtcg gccagctcca gcaagcctcg ctgctggagc gagtacccct
      781 tagaccagat cggcggttcg gcggttccac ctggggggcc ttcgagtgtg tcgagggcga
      841 ttccctcgcg gaggcgtagc ccgagcatga tggcctcggt ccacgcgttt tccggggtga
      901 tctcctccaa accctcctgg tatgagacgc cgtcggtcac gcaccgcacg tagtcgtcgt
      961 gttgtcgtgc gttccaccag cgccggccgg cgatgaacga gtgagcggcc gcgccgagtc
     1021 ccaggtattc gccgcggcgc cagtacgtca ggttgtgccg gcactcggcg cccgggcggg
     1081 cgaagttgga cacctcatag cggtcaaggc cgtactcggc cagtacatgg cctgcccggg
     1141 cgaagcacca cgcttcgtcc tcttctgaca tgggagcgat gcggccggca tcgcgcgccc
     1201 ggcgcagcgg tgtgccctcc tcgtaggtga gcgcgtaggc cgagaggtgc ggaagccctc
     1261 gccgggccag ttcgcgcaag tccgcctcga agcggtcggg ggactgaccg gggatgccgt
     1321 agatcaggtc ggccgagagg tcactaaaac ccacggcgtg ggcatcggtg atggcttgat
     1381 gggcctgggt ggctgtgtgg gtcctgccca gagcctggag gaggtcgtcg gacaatgact
     1441 gcactcccag gctcagccgg ttcgccccgg cgtcgcggag gcgtgccagt gtgtcgcggg
     1501 tgacggtgcc ggggttggct tccacggtca cctcgatgtc cggttcgacg ggcatggagt
     1561 tgaggcagac ggtgaggaga gccgccaggt cggcgggtgg cacgacggtg ggcgtgcccc
     1621 cgccgatgta gagggtcgcg agtgaccgac cctggctggc caggctcgca agctggcgct
     1681 cggcgtcggt atggagtgcg cgcatgtagg cggccacgct gtcgtggttg gctggaaccg
     1741 acgcgaagtc gcagtagagg cacttggttc gacagaaggg tatgtggatg tacacggaga
     1801 tggggcgcat gggcgtcagt cgtcgagctt gaggagggtc atgaatgcct cctgggggat
     1861 ctcgacactc cccaccatct tcatccgttt cttgccggcc ttctgcttct cgagcagctt
     1921 acgcttgcgg gtgatgtccc cgccgtagca cttggccagc acgttcttgc gatagggaga
     1981 gatggcggcg gccgcgatca cacgtccccc gatggcgccc aggatctgga tccggaactg
     2041 ctgccggggg agcacgtgct tgagccggct gacgatgtcg cgggcccgct cgtaggctcg
     2101 atcgcggtgg atgatgactg aaagggcatc gaccgggtcg ccgttgagcc agatgtcgag
     2161 ccggaccacg ttggctggac gcacgccgat gggctcatag tcgagggacc catagccccg
     2221 gctacgggat ttgagttggt cgaagaagtc cacgaggatc tcggcgagag ggagctcgta
     2281 cacgagcgtg acccgctggc cgtcatggta gtgcatgttg gagaagacgc cgcgccggtc
     2341 gccacagagc tccatgaggg gcccgacgta ttcctcaggt gtgctgatcc gggcgaggac
     2401 gtagggctcc tcgacccgga cgatccggtc ggcggtaggg aagagggacg ggttgtcgat
     2461 gtcgacggca ctaccgtcgg tcaagtggac acggtagacc acgttgggcg ccgtggcgat
     2521 gaggttggag ccgtattcgc gttcgaggcg ctcctggaca atctccatgt ggagcaagcc
     2581 gaggaagccc gcgcggaagc cgaagccaag ggcttcggag gtctccttct cgtagctgag
     2641 ggccgcgtcg ttgagctgga gcttctcgag ggcgtcgcgc aggttgtcgt attccgagtt
     2701 gtcggtgggg tagagtccgc agaacaccac gggctgggcg accttgtagc cggggagt
//
LOCUS       DAOSPL010000532         2752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_36791_length_2752_cov_4.197256, whole genome shotgun sequence.
ACCESSION   DAOSPL010000532 DAOSPL010000000
VERSION     DAOSPL010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36791_length_2752_cov_4.197256"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PLQ54_10290"
     CDS             <1..484
                     /locus_tag="PLQ54_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOS93692.1"
                     /translation="NVIPQDGPIGHGTESELPVTVTQAALQTPLARTVIDAVNEARAS
                     QGLAALSGAADLNELAQSYSERMAAEGFFSHEDPEGNRLKDRIEAAGISYLAVGENLA
                     KNRGHDDPAAVAVTSWMGSSGHRANMLNPEYTETGVGIAQSTDGTYYFTELFIKRPGS
                     "
     gene            complement(516..758)
                     /locus_tag="PLQ54_10295"
     CDS             complement(516..758)
                     /locus_tag="PLQ54_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOS93693.1"
                     /translation="MTMVGYVKWFNDEKGYGFIDIGDDDEDVFVHYTAIIEEGYRSLK
                     QGEKVRFELYEGERGPQARNVVKVSDGENHCGAISS"
     gene            985..1803
                     /locus_tag="PLQ54_10300"
     CDS             985..1803
                     /locus_tag="PLQ54_10300"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOS93694.1"
                     /translation="MRRACAGLLGLSVMAGLLSLSACRKKEPWEFGDGIPTKVEEAQK
                     QEPIPIDHFVKISTTKGDMWAELFPDMAPKTVENFLKLIDMKYYDGLTFHRIVRDFMI
                     QGGDDKKGGPGWTIKRELSAVKHCRGTLSMARTQDPDSANAQFFIVHKYSDSSKALDN
                     QYATFGRVIKGLKVIDDIVAAADSFPGAEKFPPADKCEKINSITRVSWDEIQKILPDA
                     KKEWDAEADEGVQGDPTYKKIPYWTPSSGDTGAAAPGAKATPSSPTKAEAAKKP"
     gene            1926..>2752
                     /locus_tag="PLQ54_10305"
     CDS             1926..>2752
                     /locus_tag="PLQ54_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS93695.1"
                     /translation="MASHATTAEVCERIIANVERVVVGKRRVIRLALVSLLCEGHVLL
                     EDVPGVAKTMLARSLAVSLGLQFNRVQCTPDLLPSDVTGSSVFNQKTADFEFRAGPVF
                     TNILLADEINRATPRTQSALLQAMAERQVTIDGMTHSLSRPFVVLATQNPVEHEGTFP
                     LPEAQLDRFFMRLSVGYPSFADENNMLLRVRDGHPIDSLAQVVEPEDLAAARRHARLT
                     HVDDKVREYLVRIVQATRQSPHLVLGASPRASQCLFFAAQAAAAMVGRNFVIPDDVKA
                     "
BASE COUNT          532 a          909 c          859 g          452 t
ORIGIN      
        1 gaacgtcata ccacaggacg gcccgatcgg ccacggtacc gagtccgagc tcccggtcac
       61 ggtcacccaa gcagcgctgc aaaccccgct ggcccggacg gtgatcgacg cggtcaatga
      121 ggcgcgggcc agccaggggc ttgccgcgct gtcgggcgct gccgacctga acgaactggc
      181 acagtcctac agcgagagaa tggcggccga ggggttcttc tctcacgagg acccggaggg
      241 caaccggctc aaggatcgta ttgaagcggc aggcatctcc tacctagcag taggtgagaa
      301 cctggccaag aaccgaggcc acgatgaccc cgccgcagtc gcagtcacat cgtggatggg
      361 gagctcgggc caccgcgcga acatgctcaa ccccgagtac actgagaccg gagtcggtat
      421 cgcgcagagc accgacggga cgtattactt caccgagcta ttcatcaagc gccccgggtc
      481 gtgacgggta ccctgcgcct gggaaatggt acacgctagg atgatatcgc accgcagtgg
      541 ttctccccgt cggagacttt gaccacgttc cttgcctggg ggccccgttc cccctcgtag
      601 agctcgaaac gcaccttctc cccctgcttc aggctgcggt aaccctcctc aatgatggcc
      661 gtgtagtgga cgaagacatc ctcgtcgtca tccccgatgt cgatgaaccc gtagcccttc
      721 tcgtcattga accacttcac atagcccacc atcgtcaacg ccatcacccc cagggcggtc
      781 cacatcggat gaggacccgg cgcgtccggg gcccgtcccg ccctctcgct tctactgaag
      841 cgaccgtgcc acccgagagt tcccaaggta tactgacaaa tcgccgaagc cggcgctgct
      901 ggtcacgcct ggacggcgcg ccggccgaac ccctcttgga gtcgcgcctc gggcttgccg
      961 aggctgcggg aaagggatcg aacgatgcgc agagcgtgtg ccggtcttct gggcctctcg
     1021 gtcatggcgg ggctgctatc tctctcggcg tgcaggaaga aagagccgtg ggagtttggg
     1081 gacggcatcc cgaccaaggt ggaggaggcc cagaagcagg agcccatccc gatcgatcac
     1141 ttcgtgaaga tctcgaccac caagggtgac atgtgggctg agctgtttcc tgacatggcg
     1201 ccgaagaccg ttgagaactt tctcaagctc atcgacatga agtactacga cggactgacc
     1261 ttccatcgga tcgtgcggga cttcatgatc cagggcggcg atgacaagaa gggcggcccc
     1321 ggctggacca tcaagcgcga gctctccgct gtgaagcact gccgagggac gctgtccatg
     1381 gcccggaccc aggaccccga ctccgccaac gcccagttct tcatcgtgca caagtacagc
     1441 gactcatcca aggctctcga caaccagtac gcgacctttg gccgtgtcat caagggcctc
     1501 aaggtcatcg acgacatcgt ggcagcggcg gacagcttcc cgggcgccga gaagttcccg
     1561 ccggcagaca agtgcgagaa gatcaactcc atcacccgcg tgagctggga cgagatccag
     1621 aagatcctac cggatgccaa gaaggagtgg gacgccgagg ccgacgaggg ggtacagggc
     1681 gatccgacct acaagaagat tccgtactgg actcccagtt cgggtgacac cggtgcggcc
     1741 gctccgggcg cgaaggccac tccatcatcc cccaccaagg ccgaggcggc gaagaaacct
     1801 tagcttcacc caaccgcggc acaacagaag gcatcgacga atcgggtgcg agcagcaacg
     1861 tcgccagata gagggtcgcc ggggaagcgg ttgcctcggc gaccccgcgc tctgggggga
     1921 ccccgatggc gagccatgcc acgaccgctg aagtctgcga gcggatcatc gcgaacgttg
     1981 agcgtgtagt ggtgggcaag cggcgggtca tccggctggc gctcgtatcg ctcctctgcg
     2041 aggggcacgt gttgctcgag gatgtgcccg gcgtggccaa gaccatgctg gcccgctcgc
     2101 tggcggtgtc gttggggctg cagttcaacc gggttcagtg cacgcccgac ctgctgccgt
     2161 cggatgtgac gggctcgtcg gttttcaacc agaagacggc cgacttcgag ttccgcgcgg
     2221 gacccgtctt cacgaatatc ctcctcgccg acgagatcaa ccgggccaca ccgcgtaccc
     2281 agtccgcctt gctgcaggcc atggccgagc gtcaggtcac catcgacggc atgacgcact
     2341 ccctcagccg gcccttcgtc gtgttggcga cacagaatcc cgtggagcac gagggcacct
     2401 tcccgctgcc cgaggcgcag ctcgaccggt tcttcatgcg cctcagcgtg gggtacccca
     2461 gcttcgccga cgagaacaac atgctcctcc gcgtgcgtga cggtcacccg atcgattcgt
     2521 tggcgcaggt ggtggaaccc gaggacctgg ccgccgcacg ccgccacgcc cggctaacac
     2581 acgtcgacga caaagtcagg gagtacttgg tgcgaatcgt tcaggcaaca cggcagtcac
     2641 cccacctggt tctcggggcg agcccgcgcg ccagtcagtg cctcttcttc gccgctcagg
     2701 cggcggcggc catggtgggc cgcaatttcg tgattcccga cgatgtgaag gc
//
LOCUS       DAOSPL010000533         2752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_36800_length_2752_cov_2.923619, whole genome shotgun sequence.
ACCESSION   DAOSPL010000533 DAOSPL010000000
VERSION     DAOSPL010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36800_length_2752_cov_2.923619"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="PLQ54_10310"
     CDS             <1..1207
                     /locus_tag="PLQ54_10310"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOS93696.1"
                     /translation="TADVRHLPVDAGGIERIVRVGEAERRQAEAQGGRESATLVDPQS
                     VHVADFGFARLDGKVVATGKGEGDPTQIRGVGLRISDARVNIPAFEEEDRSAPPAAAT
                     TAVATVPARRAFRFPQRDDFDIAIDVNVGNNVQVPAYQTELRGLASLYSRAGDVKLQG
                     MLVGQKGEVSYYTVRINVTSLIVTLDWSSLPGSGGEMDEFKAYLTADGNTSVISKGVR
                     TRILVHIEGPLAGESLIAGRVRYVARLASPYGDDQPINTGPLQLTLRSEPPLPQREIL
                     QLLGQGYVPGLAREGQTDYTKVLSTNVFEALASTFVGSFVSQVSDLLGLNGLTLEWNP
                     RTGINTFGIDRAITDKLYLEGWMLTASPRYRRYHLGITYDLTDHLRGGFAINETLTRR
                     VELRYQTPF"
     gene            complement(1302..1952)
                     /locus_tag="PLQ54_10315"
     CDS             complement(1302..1952)
                     /locus_tag="PLQ54_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS93697.1"
                     /translation="MRRHDTGFTLIELLVVIAIIAILAAILFPVFAKAREKAEQTSCL
                     ANMKQIALAMNMYCTSWDQRFPIGYEAGGDWGLALQPYMQNLQILVCPGAYPSPNGQG
                     ASSYVVSTGTLVGSDTHTGVMPGGWTWAAGRPLTNFKKSSYSILLYEEDTVADAYKHG
                     TNWVQAEGSATSPYTPIHMSGANYAFVDSHAKWIIGGTQPGGGAPGSTFSHGCNPL"
     gene            2316..>2752
                     /locus_tag="PLQ54_10320"
     CDS             2316..>2752
                     /locus_tag="PLQ54_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93698.1"
                     /translation="MAKTTIEHPSCVEARGDMRTPQFVVASMVLLLPLLSPSWGAPFT
                     DVPDGHPAAAAITRLVGAGILTPTGGAFQGEMVADSDFLVAVGARLIAFVSLRLVRED
                     STTPLDLYVRGRSAAMPPPVDGVPVPETHPDYAAHVFVRQVEP"
BASE COUNT          468 a          911 c          893 g          480 t
ORIGIN      
        1 gaccgcggat gtgcgtcacc tgcccgtcga tgcgggcggc atcgagcgga tcgtgcgcgt
       61 gggagaggcc gagcggcggc aagcggaggc tcagggcggc cgggagtccg ccacgctcgt
      121 tgacccccag agcgtgcatg tggccgactt cggcttcgcg cggctggacg gcaaggtggt
      181 tgccacaggc aagggcgagg gggaccccac ccagatacgg ggggtggggc ttcgcatcag
      241 tgatgccaga gtcaacatcc ctgcgttcga ggaagaggac cggagcgccc cgccagccgc
      301 cgcgaccacc gctgtggcga ccgtgccggc gcgcagagcc ttccggttcc ctcagcgcga
      361 cgacttcgac atcgcgatcg acgtcaacgt gggcaacaac gtgcaggtac ccgcctacca
      421 gacggagctg cgtggcctcg cgtccctgta cagccgcgcc ggagacgtga agctgcaggg
      481 aatgctcgtc gggcagaagg gtgaagtctc gtactacacc gtgcgcatca acgtcacgtc
      541 gctgatcgtg accctcgact ggtcgagtct gccggggagc ggcggcgaga tggacgagtt
      601 caaagcctac ctgaccgccg atggaaacac gtcggtgatc tcgaagggcg tgcgcacccg
      661 aatcctggtt cacattgagg ggcccctggc gggcgagtca ttgatcgcgg gacgtgtccg
      721 atatgtggcc cgccttgcat ctccctatgg cgacgaccag cccatcaaca cgggtcccct
      781 gcagctcacg ctccggtcgg agccgccgct cccgcagcgt gagatcctcc agctcttggg
      841 ccagggctac gttcctgggc tggcgcgcga ggggcagacg gactacacca aggttctatc
      901 gacgaacgtg ttcgaggcgc tggcgagcac ctttgtgggt agcttcgtct cccaggtcag
      961 cgacctactg ggcctcaatg gactcacact cgagtggaac ccgcgcacgg gcatcaacac
     1021 attcggcatc gatcgcgcca tcacggacaa gctgtacctc gaaggctgga tgctgaccgc
     1081 gagcccacgg taccggcgct accacttggg catcacctat gatctgaccg accacctgcg
     1141 cggggggttc gccatcaacg agaccctgac gcggcgcgtg gagctccgct accagacgcc
     1201 gttctgagtg cgacgcgact ccgaagcgtc tcggcacgcc aatagcaaag cccagccggg
     1261 tagtcggctg ggctttgcat cctgaactca tccccgaggg gctagagcgg gttacagccg
     1321 tggctgaagg tcgagccggg ggccccgccg ccaggctgcg ttccgccgat gatccacttg
     1381 gcgtggctgt ccacgaacgc atagttggcg ccagacatgt ggatgggggt gtagggcgag
     1441 gtggccgatc cttcggcctg tacccagttg gtgccgtgct tgtaggcgtc agccaccgta
     1501 tcctcctcgt agagcaggat cgagtaggat gacttcttga agttcgtcag tggtcgaccc
     1561 gcggcccatg tccacccacc cggcatgact cccgtgtggg tgtcagagcc cacgagtgtc
     1621 ccggtgctca ccacgtagct cgacgcaccc tggccgttag ggctggggta cgcgcccggg
     1681 cacaccagga tctggaggtt ctgcatgtag ggctgcaagg cgagccccca gtcgccgcca
     1741 gcctcatagc caatcgggaa ccgctggtcc caactggtgc agtacatgtt catggcgagc
     1801 gcgatctgtt tcatgttggc caggcatgag gtctgctcgg ccttctccct tgctttggcg
     1861 aagaccggaa acaggatcgc tgccaggatc gcgatgatcg cgatgacaac gagcagctca
     1921 ataagggtga agcccgtgtc gtgacgccgc atatgtttct cctctcgatc gcacacccgc
     1981 acccggtggc gcgcggtgaa cgacacccag acccagtagg gcgaatccta gccggtaagc
     2041 ggccaccacg caacgcgctc cgcagccaac cacacgggac gatccggtct ctgtcccggc
     2101 gccggtccga aagccgctgc ctgtgcccca tccggcgtcc cgaccacccc ctcgcgtccc
     2161 cacccacatg gattcacttt ggtccttatc gtcctatacc cattgtgaca gcatcgcaaa
     2221 caccggtggc ttggcagatt ggacgctcgc gtgggcatat tgtttcgtgc gcaatggccg
     2281 cgcggcgatg gcgtcgacaa ggagaccgcc ccggcttggc gaagaccacc atcgagcacc
     2341 catcctgtgt cgaagcgagg ggcgatatgc gaaccccaca gttcgtggtt gcctcgatgg
     2401 tcctccttct gccgctgctg tcgccgagct ggggggcgcc tttcactgac gttccggacg
     2461 gacatccggc ggctgccgcc atcacgcgcc tcgtgggcgc cgggattctg acgccgacag
     2521 gtggcgcatt ccagggcgag atggtggccg atagcgactt ccttgtggcg gttggcgctc
     2581 gcttgatcgc cttcgtgtcg ctccggctgg tacgggagga ctccacgacc cccctggacc
     2641 tctatgtgcg ggggcggtcg gccgcgatgc cacctcccgt ggatggcgtc cccgtccccg
     2701 agacgcaccc cgactatgcc gcccacgtgt tcgtccggca ggtcgaaccc ga
//
LOCUS       DAOSPL010000534         2749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_36849_length_2749_cov_3.060505, whole genome shotgun sequence.
ACCESSION   DAOSPL010000534 DAOSPL010000000
VERSION     DAOSPL010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36849_length_2749_cov_3.060505"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="PLQ54_10325"
     CDS             complement(<1..1133)
                     /locus_tag="PLQ54_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HOS93699.1"
                     /translation="MASVLGISAYYHDSAAALVQDGRIVAAVQEERFSRVKHDHRFPA
                     HSVRYCLEAGGITASDLDAVVFYDKPLLKFERLLETYLAFAPRGLRSFVQAMNVWLSE
                     KLFIPREIDHGLGGSYAGPIYFASHHESHAASAFYPSPFDEAAILTIDGVGEWATTAW
                     GRGSGSRIDLCAQIEFPHSLGLLYSAFTYYLGFRVNSGEYKVMGLAPYGKPRYAETIM
                     AHLLDLRDDGSFWLDQHYFNYCAGLTMTNERFHRLFDGAPRPPESPLTQRHMDVAASI
                     QHVTEEVLLRLARHVHGQTGLPNLCLAGGVALNCVANGRILREGPFQRLWIQPAAGDA
                     GGALGAALLGWYRIMDSPREPEEPDAQSGSYLGPAADPAGVPAL"
     gene            1324..1695
                     /locus_tag="PLQ54_10330"
     CDS             1324..1695
                     /locus_tag="PLQ54_10330"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOS93700.1"
                     /translation="MDYRRAAKLFDALGSDVRLRIVDLVSAHKEMCTCELVDMLRMSQ
                     ANISRHVKVLRDAGVLLDRKVGTMVVLRVDEIAIGAGFRELAELVRGNHARSALEDVQ
                     ARLRRRCESADGCDAATSNGD"
     gene            1702..1941
                     /locus_tag="PLQ54_10335"
     CDS             1702..1941
                     /locus_tag="PLQ54_10335"
                     /inference="COORDINATES: protein motif:HMM:NF021464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2180 family protein"
                     /protein_id="HOS93701.1"
                     /translation="MRCYDCAVEGRGSDAVGVCHGCGRAVCQEHAVLQHLPQHYRVSD
                     GMIGSKVRCKLDQARLVCHECEKSLDCCDQSARTA"
     gene            1975..>2749
                     /gene="arsM"
                     /locus_tag="PLQ54_10340"
     CDS             1975..>2749
                     /gene="arsM"
                     /locus_tag="PLQ54_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOS93702.1"
                     /translation="MPEDSQTIKDAVRAAYAGVARGSGCCCGGAPAVDLTEFGQALGY
                     SASDLEGVPDGANLGLGCGAPLEYAELQPGETVLDLGSGAGFDCFVARRTVGDSGRVI
                     GVDMTPEMIARARANARQAGYDNVEFRLGEIEALPVADASVDLVISNCVLNLVPDKKA
                     AFREIARVLKPGGRMVVSDIVRVGELPARLRDSMAAYAACASGALPRDEYLAGLVQAG
                     LKGIEVLRQVEARPMFVTGACCGELAGLEEGILASATIRA"
BASE COUNT          445 a          858 c          971 g          475 t
ORIGIN      
        1 agagccggca cgcccgcagg atccgccgcc gggcccaggt aggatccgct ctgggcatcg
       61 ggctcctctg gctcgcgcgg gctatccata atgcggtacc aacccaacag cgccgcgccc
      121 agcgcccctc cagcatcgcc ggccgcgggc tggatccaga gacgctggaa gggcccctcg
      181 cgcaggatac ggccattggc gacgcagttg agtgcgacgc cacccgccag gcacaggttc
      241 ggtagacctg tctggccatg cacatggcgt gccagccgga gcaggacctc ctcggtcacg
      301 tgctggatgc tggccgccac gtccatgtgc cgctgtgtga ggggtgactc gggcggtcgg
      361 ggcgcgccgt cgaacagccg atggaagcgt tcgttcgtca tcgtcagccc agcgcagtag
      421 ttgaagtagt gctggtcgag ccagaaggaa ccgtcatccc tcaagtcgag cagatgggcc
      481 atgatggtct ccgcatagcg cggcttgccg tagggggcca gacccatcac cttgtattcg
      541 cccgagttca cccggaaccc gaggtagtac gtgaacgcgc tgtagagcag accgagtgag
      601 tgtgggaact cgatctgtgc gcacaagtcg atccggctcc cgctgccgcg accccaggcc
      661 gtggtcgccc actcgcccac cccgtcgatg gtcaggatgg cggcctcgtc gaagggcgag
      721 gggtagaacg cactcgccgc gtgactctcg tggtggctgg cgaagtagat ggggcccgca
      781 tagcttccac cgaggccatg gtcgatttcg cggggaatga acagcttctc cgagagccac
      841 acgttcatcg cctgcacgaa cgagcgcagc cctcttggcg cgaaggccag gtaggtctcg
      901 aggaggcgtt cgaacttcag gagtggctta tcgtagaaca cgaccgcgtc gagatccgat
      961 gcagtgatgc cacctgcctc gaggcagtag cgcacgctgt gcgcggggaa tcggtggtca
     1021 tgcttgaccc ggctgaaccg ttcctcctgg accgcggcaa cgatacggcc atcctggacc
     1081 agggcagcgg cactgtcgtg gtagtaggct gaaatgccga ggaccgatgc catctgctcc
     1141 gcgccgctcc acatgcgagt cggccgcgcc tccgatccgt ccgatgtagc cccggggcga
     1201 ttatagcgcg tcggcgctgg ccgcctccgc cgagctggag ccgtggccgc gaggagccgc
     1261 tgcttgcgtc caacgtccat tccatcgcat aatggcattg gttcgatgac ggagggtggg
     1321 ccggtggact acagacgtgc ggccaagctc ttcgacgcgc tcgggagcga tgttcgcctg
     1381 aggatcgtgg acctggtctc ggctcacaag gagatgtgta cgtgtgagct cgttgacatg
     1441 ctccgcatga gccaggccaa catcagccgc cacgtgaagg tgctccgcga cgcgggcgtc
     1501 ctcctcgacc gcaaggtcgg cacgatggtg gtcctgcggg tcgatgagat tgccatcggc
     1561 gcgggtttcc gcgagttggc tgagctcgtc cgaggcaacc acgcgagatc cgcgctggag
     1621 gacgtccagg ctcggttgcg caggcggtgc gagagtgccg acgggtgtga cgccgcgacc
     1681 tcgaatggag actgagcaac catgcggtgc tatgactgcg ccgtcgaggg caggggctct
     1741 gacgcggtgg gtgtgtgcca cggttgcggg cgtgctgtct gccaggagca cgcagtgctg
     1801 cagcacctgc cccagcacta ccgagttagc gacgggatga tcggctccaa ggtccggtgc
     1861 aagctcgacc aagcccgcct ggtgtgccat gagtgcgaga agtcgctcga ctgctgcgac
     1921 cagtcggcgc ggaccgcgta gacggtctcg tccatgccca aggggggcgg ccagatgcca
     1981 gaggattcac agacgattaa ggacgcggtt cgcgcggcct atgcgggagt cgcccgaggc
     2041 tctgggtgct gctgcggcgg tgctccggca gtggacctga cggaatttgg gcaggccctc
     2101 ggttactcgg cgagcgatct ggagggcgtg cccgatggcg ccaatctggg tctcgggtgc
     2161 ggtgcgccgc tggagtacgc cgagcttcag ccgggagaga cggtactcga cctcggtagc
     2221 ggggccggct ttgattgctt cgtggctcgg cgtactgttg gtgattccgg ccgcgtcatc
     2281 ggcgtggaca tgactccgga gatgatcgcc agggcccgag ccaacgcgcg gcaggcgggc
     2341 tacgataacg tcgagttccg gctgggcgag atcgaggctc tcccggtagc cgatgcatca
     2401 gtcgacctgg tcatctccaa ctgcgtgctg aacctggtgc cggacaagaa ggccgcgttc
     2461 cgcgagatcg cgcgggttct caagccgggc gggcgaatgg tggtgtccga catcgtccgt
     2521 gtcggcgagc tccctgcgag gctccgcgat tccatggcgg cgtacgccgc gtgcgcctcg
     2581 ggcgctttgc cccgagacga gtatctggcg ggtctcgtcc aagcggggct caaaggcatc
     2641 gaggtgctcc gccaggtgga ggctcgcccc atgttcgtga ccggcgcctg ctgtggcgag
     2701 ctggcgggcc tggaggaggg catcctggcc tctgccacca tccgcgcct
//
LOCUS       DAOSPL010000535         2748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_36871_length_2748_cov_2.812477, whole genome shotgun sequence.
ACCESSION   DAOSPL010000535 DAOSPL010000000
VERSION     DAOSPL010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36871_length_2748_cov_2.812477"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1597
                     /locus_tag="PLQ54_10345"
     CDS             <1..1597
                     /locus_tag="PLQ54_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93703.1"
                     /translation="AVGSSEQLADTRSITDALDYVGANFYWDHPTWPAGREWQDPYFV
                     NQEPPLRSDGGRSLGPALAVALTARKPLVVREWTYCWPNPARATGVLEMAAFCSLQEI
                     DAALAFTYQAADGGRPIDYFDFRADPARWGPFAVAGEVFAGRHLRPGQLRVDVSFGPD
                     DVFASVQGLNPVHRAAWISRLRNRFDDGQVPAEADLTVLAGRSPGRGADGRGLILPGS
                     VAGGPVPPAMESEMGWLSGYAVPPGRPPGDDSPQPVLFDGLIADGRRTGYDLTRTYDL
                     DAIGKAGFVPIGTNGSLQSALGFHDDAKGNFVFRNLPDHDLAALVFDVLGRRAQRLSA
                     AERVASHRTLEEGITISDTGEIIRDAGQGLLTVNAPTARMIAASDASALSAKAGDLEL
                     RTHDRCCALTAVALDGKPLFESRSYVVRMVTVAENQRQQVARVTGGPKPYAVMRRGEA
                     PPTTLGQPSQTATTLLLGGYPLLRVRQRNGSWDLVRIGDRFWFMTDAEGIAVSVAGGP
                     ATPCQAHRPIAFDDRGMMVPPPA"
     gene            1907..>2748
                     /locus_tag="PLQ54_10350"
     CDS             1907..>2748
                     /locus_tag="PLQ54_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93704.1"
                     /translation="MSTRSGRRAYPHSAVLWVAVVALAAAAVPSHAWWMETQLAGIRL
                     GDNAMILLDIPGYGQPDGIIVATATSADRANVPGAEGGQGPGLTGPGGTGAAGPGAAG
                     MMGPGAAGMMGPGGGPMGSGPGMMGGGMGPGMARGSTRSIPSAVAPVTPVQMAMGMAA
                     PMGSMGSGGGMGMPPGQIAAGATGPMAGAGGMMGGQPGMMGGAGPGPGMMGMPGMAGP
                     GGAAGAAGAGAGGFGPAQGVSDFPDWVMAIWFDLHEDEIEWFYMRDKCAVGFVINKNS
                     GKIVA"
BASE COUNT          414 a          920 c          982 g          432 t
ORIGIN      
        1 agccgtcggc agctcggagc agttggccga tacccggtcc atcaccgatg cgctcgacta
       61 cgtcggcgcc aacttctact gggaccatcc cacatggccc gccgggcgcg agtggcagga
      121 tccctatttc gtgaaccagg agccgccact acgctccgac ggcggccgga gcctggggcc
      181 ggcactggcc gtggcactta cggcgcggaa gcctctggtg gtgcgcgagt ggacctactg
      241 ctggccgaac ccggcccgag ccaccggcgt gctcgaaatg gccgcattct gctccctgca
      301 ggagatcgat gcggcgctgg cattcaccta ccaggcggcc gatggaggca gacccatcga
      361 ctacttcgac ttccgggctg acccggcccg atggggtccc tttgcggtgg cgggcgaggt
      421 gttcgccggc cgccatctgc gccccggcca gctgagggtc gatgtgtcct ttggcccaga
      481 cgacgtgttc gcgtcggttc agggtctgaa ccccgtccac cgggccgcat ggatctcgcg
      541 gctgcggaac cggttcgacg acggccaggt accggccgag gccgacctga ccgtcctggc
      601 cggacgcagc cccggtcgcg gcgccgacgg ccgtggcttg atcctccccg gctccgtcgc
      661 cggcggcccg gtgcccccgg ccatggagtc cgagatgggc tggctctccg gctacgcggt
      721 gccccccggg cggccgccgg gcgacgactc gccgcaaccc gtcttgttcg acggtctcat
      781 cgccgacggc cgccgtaccg gctacgacct cacccgcacg tacgacctcg atgccatcgg
      841 gaaagccggg ttcgtcccga tcggaaccaa cggctcactc cagtccgccc tcggcttcca
      901 cgacgacgcc aagggcaact tcgtgttccg caacctcccc gaccatgatc tggcagccct
      961 cgtgttcgac gtgctcggcc gtcgagcgca acggctgagc gccgccgagc gggtcgcgtc
     1021 gcaccgaact ctcgaagagg gcatcaccat ctcagatacg ggtgagatca tccgtgatgc
     1081 cggccaggga ctgctcacgg tgaacgcccc caccgcgagg atgatcgccg caagtgacgc
     1141 cagtgccctc tctgccaagg ccggcgacct ggagctccga acgcacgacc gatgctgcgc
     1201 cctgactgcg gtggcgctgg acggcaagcc cctgttcgag tcgcgatcgt acgtcgtccg
     1261 tatggtcacc gtggcggaaa accagcgaca gcaggtcgcg cgagtgaccg gaggccccaa
     1321 gccctacgcc gtgatgcgga gaggcgaggc accgcccacc acccttggcc agccgagtca
     1381 gacggcgacc accctcctgc tgggcggcta tccgctgctc cgcgtgcggc aacgcaacgg
     1441 gtcgtgggac ctggtacgca tcggggaccg attctggttc atgaccgacg ccgaggggat
     1501 tgcggtctcg gtggccggcg ggccagccac gccttgccag gcccaccggc ccatcgcctt
     1561 cgacgaccgg ggcatgatgg tacctccgcc agcctgaccg cggccatttg gcgcccatcc
     1621 ggttgtgtac ccgaccgcga aatgggaagg attgagggca ggagaaaacc agaaccctgc
     1681 gaaaggcatt atacgatcag gtaggagtcg ggcatgtgtg cgccgaatgg catcgccacg
     1741 gctcctggat gccgcgggcg ctggcctcgc gggcggtggc ggcgtgggag catcatgcga
     1801 tggcagttgc ctgccaggct gaactgagcg gatgtggcga tgggttctcg gcgtgcaaga
     1861 tgctgacagc gcgcccagtt tgtcctggct ggcgggaggc gacttgatga gcacgaggtc
     1921 tggtcggcgg gcttacccgc acagtgcggt cttgtgggtc gcggttgtgg cactggctgc
     1981 tgcggccgtt ccatctcacg catggtggat ggagacccag cttgcgggca tacgcctggg
     2041 cgacaacgca atgattctgc tcgacatccc gggctacggc cagccggacg ggattatcgt
     2101 cgcgacggcc acctcggccg atcgggccaa cgtgcccggt gccgaggggg gccaaggccc
     2161 cggcctgacg ggtcccggcg ggaccggtgc ggccggcccc ggtgctgcgg gtatgatggg
     2221 ccccggtgcc gctggcatga tgggccccgg cggcggaccg atgggctcgg gtcccgggat
     2281 gatgggcggc ggcatggggc cgggcatggc gcggggaagc actcggagca tcccttccgc
     2341 cgtggcaccg gtcacgcccg tgcagatggc catgggaatg gctgcgccga tgggtagcat
     2401 gggtagcggc ggcggcatgg ggatgcctcc cggccaaatc gctgccggcg ccacgggccc
     2461 catggcagga gccggtggca tgatgggggg ccagcccggc atgatgggcg gtgccggtcc
     2521 cggtcccggg atgatgggta tgcccggaat ggccggcccc ggtggcgccg cgggcgcggc
     2581 gggtgcggga gccggcggct ttggcccggc ccagggagtg agcgacttcc ccgattgggt
     2641 catggccatc tggtttgacc tgcacgagga cgagatcgaa tggttctaca tgcgcgacaa
     2701 gtgtgccgtg ggatttgtca tcaataagaa ctcgggcaag atcgtcgc
//
LOCUS       DAOSPL010000536         2746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_36910_length_2746_cov_3.125604, whole genome shotgun sequence.
ACCESSION   DAOSPL010000536 DAOSPL010000000
VERSION     DAOSPL010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36910_length_2746_cov_3.125604"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>533
                     /locus_tag="PLQ54_10355"
     CDS             <1..>533
                     /locus_tag="PLQ54_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93705.1"
                     /translation="SGSCNGNFLGIGADASGQAVRVEQCQGMGLLITNGEFVAMLGED
                     PISVVTTSTCTGDVSLTNCAFWGPSRQLLRHEGTGTVTLTGCNFREWDSQGKGRPALE
                     ALGGSLVVQGCNFTKGGNHVVLGEGAVSAAIVGNRFAAEPHIENRSEGGVEIGLNAVS
                     RVVSGEITLGGPNRGRNV"
     assembly_gap    534..543
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <544..937
                     /locus_tag="PLQ54_10360"
     CDS             <544..937
                     /locus_tag="PLQ54_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93706.1"
                     /translation="ADCGTEVKELAGRTCRIATGMYILFGVDSALAGDGAHPAVVIEV
                     DYLDEGAGVFFLEYDSTDETVLKVAERPGAFKGTEPVELTGSGEWRTATFRLPDARFS
                     DRCNGGDFRITSPEAHMAVSAVRVRRGE"
     gene            1026..2306
                     /locus_tag="PLQ54_10365"
     CDS             1026..2306
                     /locus_tag="PLQ54_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOS93707.1"
                     /translation="MSGTTLWWPTCAAVAWLAAGGAEGEEPIYDEARVPPYTLPDPLQ
                     LANGSRVRDAETWDNERRPELLKLFETHVYGRSPAPPAWVPFDVVESSADALGGLATR
                     KQIVFDPTGHRGHLEVDMLLYLPNAAHGPVPVFLALNFEGNACICDDPTIRLPRSWMR
                     EGNGVVDHRATEALRGVETRHWQVEQVLSRGYGIATIYYGDIDPDFDDGFQNGIHPFF
                     YRAGQTAPEADEWGSIGAWAYGLSRALDVLEHDPKVDAKRVAVLGHSRLGKTALWAGA
                     QDERFALVISNNSGCGGAALSRRCFGETVGRINRAFPHWFCDNFSEYNENEAALPVDQ
                     HELIALIAPRPVYVASAEEDKWADPRGEFLAALGADPVYRLLGTDGLPAREMPAVNEP
                     VHGTIGYHIRSGGHEVAPYDWEQYLAFADRHLQR"
     gene            2510..>2746
                     /locus_tag="PLQ54_10370"
     CDS             2510..>2746
                     /locus_tag="PLQ54_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93708.1"
                     /translation="MGPTVVALTLWPGLALAGVLAEYHVSALAPNASDAAAGTAEQPW
                     ATISHAAEVLEAGDRVIVHAGTYREHVLPRHSGRE"
BASE COUNT          433 a          873 c         1018 g          412 t
ORIGIN      
        1 agcggctcct gtaacggcaa cttcctcggc atcggcgcgg atgcttcggg ccaggcggtg
       61 cgggtcgagc agtgccaggg catgggactg ctgatcacca acggcgagtt cgtggctatg
      121 ctgggtgagg acccgatctc ggtcgtgacc acctcgacct gcacgggcga tgtgagtctg
      181 acgaactgcg ccttctgggg cccgagccgg cagctcctgc ggcacgaggg cacgggcacg
      241 gtgacgctga cgggctgcaa cttccgcgaa tgggattcgc agggcaaggg ccggccggcg
      301 ctggaggccc tgggcggctc gctggtcgtg cagggctgca acttcaccaa gggcgggaat
      361 cacgtcgtgc tgggcgaggg cgccgtgagc gctgctatcg tgggcaaccg cttcgccgct
      421 gagccccaca tcgagaaccg cagcgagggc ggggtcgaga tcggcctgaa cgccgtcagc
      481 cgcgtggtga gcggtgagat cacgctgggg ggtccgaacc gcggccgcaa tgtnnnnnnn
      541 nnnggccgac tgcggcaccg aggtgaagga gctggcaggg cggacctgcc gcatcgccac
      601 gggcatgtac atcctgttcg gcgtcgatag tgcgctggct ggcgatgggg cacacccagc
      661 tgtcgtgatc gaggtggact acctcgatga gggcgcgggg gtgttcttcc tcgagtacga
      721 ctccaccgac gagacggtgc tcaaggtggc ggagcgaccg ggagccttca aggggaccga
      781 gccggtggaa ctcactggca gtggcgagtg gcggacggcg accttcaggc tgcccgatgc
      841 ccgtttctcc gaccgctgca acggcggcga cttccgcatc acgagccccg aggcgcatat
      901 ggctgtgtcg gcggtgcggg tgagaagggg ggagtagggg agcagtcccc gggacaaagc
      961 ttcgggcgca ggccccctgc gcactcacgt cgaacctccc tgcgaccaac gagggaggta
     1021 cgcgtatgag cggaacgacc ttgtggtggc ccacatgcgc ggcggtggcg tggttggcgg
     1081 caggcggggc tgagggcgag gagcccatct acgacgaggc gagagtgcct ccgtacaccc
     1141 ttccggatcc gctccagctc gcgaacggat cgcgggtgcg cgacgctgag acctgggaca
     1201 acgaacggcg gccggagctg ctgaagctct tcgagaccca cgtctatggc cggagccctg
     1261 caccgcccgc ctgggtgccg ttcgatgtgg tcgagtcgtc ggcggacgca ctcggcggtt
     1321 tggccacacg gaagcagatc gtgttcgacc ccaccggaca tcggggccac ctcgaggtcg
     1381 acatgctgct ctacctgccg aatgcggccc acggccctgt acccgtgttc ctggcactga
     1441 acttcgaggg caatgcgtgc atctgcgatg accccaccat ccgcttgccg cgctcgtgga
     1501 tgcgtgaagg caacggcgtg gtggaccacc gggcgaccga ggcgctccgc ggcgtcgaga
     1561 cgcggcactg gcaggtcgag caggtgctct cgcgcggtta cgggatcgcc accatctact
     1621 acggcgatat cgaccccgac ttcgacgacg gcttccagaa cggcattcac cccttcttct
     1681 accgagcagg gcagacggcg ccggaggccg acgagtgggg ttccatcggc gcctgggcct
     1741 acgggctgtc gcgggcgctg gatgtgctcg agcacgaccc gaaggtggac gcgaagcggg
     1801 tggcagttct cggccacagc cgcctgggca agacggcgct gtgggcgggc gcgcaggatg
     1861 agcggttcgc gctggtcatc agcaacaact cgggctgcgg cggcgcagca ctctcgcgcc
     1921 ggtgttttgg cgagaccgtg gggcgcatca atcgggcatt cccgcactgg ttctgcgaca
     1981 acttcagcga gtacaacgag aacgaggcgg cgctgccggt ggaccagcac gagctgattg
     2041 cgctgatcgc tccgcgcccc gtctatgtgg cgtcggccga agaggacaaa tgggccgatc
     2101 cgcgcggcga gttcctggcg gcgctggggg ctgatccggt gtaccggcta ctcggcaccg
     2161 acgggctgcc cgcgcgcgag atgcccgcag tgaacgagcc cgtacacggc accatcgggt
     2221 accacatccg cagcggcggg cacgaggtgg ctccatacga ctgggagcag tatctcgcgt
     2281 tcgcggacag gcacctgcaa aggtagtgcg gagagctgag cgaccggccg ccccgggccg
     2341 aatgggggct tcgggagcgc aggcttcagc ctgctggggc gcggggcggc gccccgcggt
     2401 ctgggctctt gggccggacg gacgcgaggg tgcggctcga tcggtgcaag accgaccggg
     2461 ccggatgggg cccccgcccc attcggcccc tccgaacggc gaggtgggta tgggaccgac
     2521 tgtcgtggcg ctcaccctct ggcccggctt ggcgttggcc ggcgtcttag ccgagtacca
     2581 cgtcagtgcc ctggcaccga acgcatcgga cgcggcggct ggcaccgccg agcagccgtg
     2641 ggctacgatc tcgcacgccg ccgaggtact cgaggccggt gaccgcgtca tcgtgcacgc
     2701 aggcacctac cgcgagcacg tgctgccgag gcactcgggc cgggag
//
LOCUS       DAOSPL010000537         2743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_36959_length_2743_cov_3.100818, whole genome shotgun sequence.
ACCESSION   DAOSPL010000537 DAOSPL010000000
VERSION     DAOSPL010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36959_length_2743_cov_3.100818"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="PLQ54_10375"
     CDS             complement(<1..921)
                     /locus_tag="PLQ54_10375"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOS93709.1"
                     /translation="MILTRRQLIHAGAGALGLSALGGRPRACHAQSTASGEPAMEFAE
                     VKYQDPATKTYLGSPSLLKLDNGDLLATHDYFGPGCPRNHEDEEHLTSVYRSSDGGGT
                     WTNVTHIAGAFWSSLFLHRGAIYLLGTSQQYGSIVIRRSDDGGNTWTHPKDSKHGLLL
                     RGGPYRDPPNYHCAPVPICLTNGRIYRAFEDCTPCKWPQGFQSLVISAPEDANLLDAA
                     SWTASNKLPFDPAWVPTEWGQLDAPGWLEGNVVETPSGELWNILRLNSAPLVDRAAVV
                     KVEDEGRRVSFDPATGFIDFPGGMSKFTIRR"
     gene            complement(918..1235)
                     /locus_tag="PLQ54_10380"
     CDS             complement(918..1235)
                     /locus_tag="PLQ54_10380"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOS93710.1"
                     /translation="MLESALAAMRDLGGMSAEQLRASPAVARSIIRCLEIVGEAAAQV
                     SPETRASADHVPRADVVAMRNRLVHAYWDVDLDIVWDTVTMHLPDLQRQLPGLLEDEG
                     EGP"
     gene            complement(1255..1566)
                     /locus_tag="PLQ54_10385"
     CDS             complement(1255..1566)
                     /locus_tag="PLQ54_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOS93711.1"
                     /translation="MGVDALRVVPEAELAALRERYGVRSLRLFGSALRGALRSDSDLD
                     VLVEFRPDQRTGFFRFMRLQAELTTLLGRQVDLHTPASLSRHFREEVLRTAEVISHNP
                     N"
     gene            complement(1588..2391)
                     /gene="trpA"
                     /locus_tag="PLQ54_10390"
     CDS             complement(1588..2391)
                     /gene="trpA"
                     /locus_tag="PLQ54_10390"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOS93712.1"
                     /translation="MGRLASRFAELKARNEGALVAYVTAGDPSIGRSLDVARAIVDSG
                     ADILELGVPFSDPVADGPTIQVASQRALEAGTTPRKVLDLVARLREDTQVPVCLLAYG
                     NIVHRYGYARFAADAAAAGVDGVLVCDLPPADDDEWSTSAAGAGIDTIYMVAPTSTTL
                     TLEQVGRRASGFVYCVSRTGTTGTRAELPPDLEQLVRRVRERAAVPVCVGFGISTPDH
                     VRAVCRMADGAIVGSAVVRVVEEHGTSADLPEAVASLCRQLKEATRGGR"
     gene            complement(2392..>2743)
                     /locus_tag="PLQ54_10395"
     CDS             complement(2392..>2743)
                     /locus_tag="PLQ54_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOS93713.1"
                     /translation="ARTYFIQDESGQIMPTHSVSAGLDYPAVGPEHSYLKDSKRARYI
                     SADDTQALHAFRYLSETEGIIPALESAHAVAGLLRMAPDLGPDAVVIVNLSGRGDKDV
                     QEVAAIMGTSRGVA"
BASE COUNT          438 a          914 c          939 g          452 t
ORIGIN      
        1 ccgccgaatg gtgaacttgc tcatcccgcc cgggaagtcg atgaagccgg tggccgggtc
       61 gaacgagacg cggcggccct cgtcttcgac cttcaccacg gcggcgcggt cgaccagggg
      121 cgccgagttg agccggagga tgttccacag ctcgcccgag ggcgtctcga ccacattccc
      181 ctcgagccag ccgggcgcgt cgagctggcc ccactcagtg gggacccatg cggggtcgaa
      241 tgggagcttg ttgctggcgg tccaactcgc tgcatcgagg aggttggcgt cctccggcgc
      301 cgagatgacc agcgactgga acccctgcgg ccacttgcag ggggtgcagt cctcgaatgc
      361 gcggtagatg cgcccgtttg tgaggcagat cggcacggga gcgcagtggt agttgggcgg
      421 gtcgcgatag ggcccgcccc ggagcagcaa gccgtgcttg ctgtccttgg ggtgagtcca
      481 cgtgttgccg ccgtcgtcgc tccggcggat gacgatggag ccgtactgct gcgacgtccc
      541 gagcaggtat atggccccac ggtggaggaa gagactactc cagaacgccc cagcgatgtg
      601 ggttacgttg gtccacgtgc cccccccgtc ggaggatcgg tagacgctgg tcaggtgctc
      661 ttcgtcctcg tggtttcgcg ggcatcccgg gccgaagtag tcgtgtgtgg ccagcaggtc
      721 gccgttgtcg agcttcagca agcttggact gccgaggtag gtcttggtgg ccgggtcttg
      781 gtacttcacc tcagcgaact ccatggcggg ctccccactg gcagtgctct gagcatgaca
      841 cgcgcgcgga cgcccgccca atgcagagag ccccagggcg ccggcgcccg cgtggatgag
      901 ttggcggcgg gtgaggatca cggcccctcg ccctcgtcct cgagaaggcc aggaagctgg
      961 cgctgcaggt caggcaagtg catagtgact gtgtcccaca cgatatccag atcgacatcc
     1021 cagtaggcgt ggaccaggcg gttccgcatc gccaccacgt cggcccgggg cacgtggtcc
     1081 gcgctagcgc gtgtctctgg tgagacttgc gcggctgcct cgcccactat ctcgagacag
     1141 cggatgatcg accgcgcgac tgccgggctc gcccgaagct gctctgccga catgccaccg
     1201 aggtcgcgca tcgccgccag agcggactcc agcatatgct ccaggcgaac gcggtcagtt
     1261 ggggttatga gagatgacct ccgcagtgcg gagaacctcc tcgcggaagt ggcggctcag
     1321 gctggccggc gtgtgaaggt cgacttgacg tcccagcagc gtggtcagtt ccgcctgaag
     1381 ccgcatgaac cggaagaagc ctgtccgctg gtccgggcgg aactcgacga ggacatccag
     1441 gtcactgtcg gagcggagtg cgccacggag agccgaaccg aacagacgga gcgagcgcac
     1501 gccgtagcgt tcgcgtaggg ctgcgagctc ggcctctggc accacccgca gagcatcgac
     1561 acccatcagc ctcgacctcc cggccgccta gcgcccaccg cgcgtggcct ccttcaactg
     1621 ccggcacagc gatgccacgg cctcgggcag gtcggcagac gtgccgtgtt cctcgacgac
     1681 ccgtaccacc gcactgccca cgatggcgcc atcagccatg cggcacaccg cgcgcacgtg
     1741 gtcgggcgtc gagataccga accccacaca gaccgggacc gcggcgcgtt cgcgcactcg
     1801 ccggacgagt tgctccagat cgggcggcag ctcggcgcgc gtgcccgtgg tgccggttct
     1861 cgacacacag tatacgaagc cggaggcccg gcgacctacc tgttccagcg tgagcgtggt
     1921 gctcgtcggt gccaccatgt agatggtgtc gatgccggcc cctgcggcgc tggtcgacca
     1981 ctcgtcatcg tcggcgggcg gcaggtcaca cacgagtacg ccatccacgc cggcggcggc
     2041 cgcatcggcg gcgaaccgcg catagccata ccggtggacg atgttcccgt atgccaacag
     2101 gcacacgggt acctgagtgt cctcccggag cctcgccacg aggtcgagga ccttcctcgg
     2161 ggtggtgccc gcctctagtg ctctctggct cgccacctgg atcgtcggcc catcggccac
     2221 ggggtcgctg aacggcacgc cgagttcgag gatgtccgct cccgagtcca cgatggctct
     2281 cgcgacatcg agggatcgcc cgatggaagg atcgcctgcg gtgacatagg ccaccagggc
     2341 cccctcgttg cgggccttca gctccgcgaa ccgcgatgcg aggcgtccca tctacgccac
     2401 ccccctgctc gtgcccatga tggcggccac ttcctgcacg tctttgtcgc ctcgccccga
     2461 gaggttgacg atgacgacgg catcgggccc caggtcgggc gccatgcgca gcagtcccgc
     2521 tacggcatgg gccgattcga gcgcggggat gatgccctcg gtttcggaga gatagcggaa
     2581 cgcgtgtagt gcctgggtgt cgtcggccga gatgtaccgc gcacgcttcg agtccttgag
     2641 gtagctgtgc tcgggcccga cggcggggta gtccaagcca gccgagaccg agtgtgtggg
     2701 catgatctgc ccggactcgt cctggatgaa gtaagtgcga gct
//
LOCUS       DAOSPL010000538         2743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_36961_length_2743_cov_3.076637, whole genome shotgun sequence.
ACCESSION   DAOSPL010000538 DAOSPL010000000
VERSION     DAOSPL010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36961_length_2743_cov_3.076637"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..318
                     /locus_tag="PLQ54_10400"
     CDS             22..318
                     /locus_tag="PLQ54_10400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS93714.1"
                     /translation="MEVDAAGQGIECRVTDCGPGITPDHLSRVFDRFYRVDKARSRDL
                     GGAGLGLSICQWIAQAHGGWIRAESEPGVRTCFAVWLPTEPPGQSRGPAEFPGR"
     gene            437..1081
                     /locus_tag="PLQ54_10405"
     CDS             437..1081
                     /locus_tag="PLQ54_10405"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOS93715.1"
                     /translation="MTSEPRGFAGIRTLGERGWMVRAGAVLQLWRGRLHALAALLGVM
                     AARPSPGWLPWELLSIGGGLLLRSWALGHLRKNEELCTSGPYAFTRNPLYLGNGLILC
                     GFCIAAGSVWFALAAWVGGVLVCRAVVLHEEELLLRKFGERYGAYRCHVPALIPRITR
                     CPGSVRAPFSWRQASRNHIAAGWIAVILVLVAMHVAGPVAAAWHPSLHLLARLP"
     gene            1269..2621
                     /locus_tag="PLQ54_10410"
     CDS             1269..2621
                     /locus_tag="PLQ54_10410"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOS93716.1"
                     /translation="MSFPRLLPCVWMACALGTARAPAVSAAEGGAPKPCGIEEAIGEA
                     LAHNASLQAAAARVDEAQAAARVADAIRHFAWRYEGAASGIVEQNIDPEYWFAHAELL
                     TSVTTTLSDGGKSKADWLRAIAEVVVARAEYEKQASSLALAVVSAFFGVVGAEAELGA
                     AREGCELVGEHLRMARARFAEGQGPELDVLRAEAALSQAQAAQVGAAAESDSARRALA
                     VLLGRPPSELVEVAEAPVGADETVADAELASAQARERAPEVRIGQAELALAQAGVEVA
                     RKTNKPDVGIVLSHTLGNDATDSRHVLQAGLRWGLPLGDHGERSARVDEGAARVRAAV
                     ALLEEASRQAEVAARDALGALAAARLRLEGLTRAAEVARTVHERATRGYADRAIAMRD
                     LLDAREDLTAAERDRVRAQCDVAVQEARLRQVLGTLVSSSSVATSTAHEATDRRGRAY
                     "
BASE COUNT          371 a          898 c          999 g          475 t
ORIGIN      
        1 gctcatgcgg ggcctgtctc tgtggaagta gacgcagccg gccagggcat cgagtgccgt
       61 gtcacggact gtggtccagg catcacgccc gatcacctgt cgcgtgtgtt cgatcgtttc
      121 taccgggtcg acaaggcgcg atcccgtgac ctcggagggg cgggccttgg tctgagtatc
      181 tgccagtgga tcgcgcaagc gcatgggggc tggatccgcg ccgagagcga acccggtgta
      241 cgcacatgct tcgccgtctg gctcccaaca gagccccccg gccagtcgag gggcccggcc
      301 gagttccctg gccgttagcg ctttttcatc ctcaccccat cgaaggttca ctcacggtca
      361 ccatactcca cctcagttgc gaacgctcgt gcttcagcgc gtgagccgtg agcagtcgga
      421 tcgtggagga tggactatga caagtgagcc gcgtggcttc gcgggcatca ggacattggg
      481 cgaacggggc tggatggtgc gggccggcgc ggtgctgcag ctgtggcgcg gccgcctcca
      541 cgccctggcc gcccttctgg gtgtgatggc ggcgcgacct tcgcccggct ggttgccgtg
      601 ggaactgcta tccatcggcg gcggcctcct tctgcgttcg tgggccctgg gccacctacg
      661 caagaacgag gagctgtgca cgtccggccc ctacgcgttc acacggaacc ccctctatct
      721 cggcaacggc ctgattctgt gtgggttctg cattgcggcc gggagcgtgt ggttcgccct
      781 ggcggcctgg gttggcgggg tgctggtgtg ccgcgcggtg gtgctgcacg aggaggagct
      841 gctactccgg aagttcggag agcgctacgg ggcctaccgg tgtcatgtgc cggcactgat
      901 tcctcgcatc acacggtgcc cgggcagtgt ccgggcgccg ttctcgtggc gccaggcgtc
      961 gcggaaccac atcgctgccg gatggatagc tgtcatcctc gttctggtgg cgatgcacgt
     1021 tgccgggccg gtcgccgccg cgtggcaccc ctcgctccac ctgctcgctc ggctgcctta
     1081 gagcccgagt ggggtgaggg caggcgtctg agctccgcca ctcatgaagg attcttaacc
     1141 cttggcccat cgtgctttca catgtcccga gcacactgcc ccagtgtggt aggtccgctg
     1201 cgccgcggcc aggcgattgg gccgcttcgg ggatcgggcc tccgcagccc agaggagtgg
     1261 gtacctggtt gtcgttcccg cggttacttc cctgtgtctg gatggcatgt gccctcggaa
     1321 ccgcccgtgc gccggcggtc tctgcagccg aagggggcgc ccccaagccg tgcggcatcg
     1381 aggaggccat cggcgaggcg ctggcccaca acgcatcgct ccaggctgcc gcagctcgag
     1441 tcgatgaggc ccaagccgcc gctcgggtcg cggacgccat ccgtcacttc gcctggcgct
     1501 acgaaggggc ggcctcgggg atcgtggagc agaatatcga ccccgagtac tggttcgccc
     1561 acgccgagct gctgacttcg gtgaccacca ccctctccga tggcgggaag tcgaaggccg
     1621 attggcttcg agccatcgct gaagtggtcg tggcccgggc cgagtatgag aaacaagctt
     1681 cgagcctggc tttggcagtc gtgtccgcct ttttcggcgt ggtcggagcc gaggccgagc
     1741 tgggtgccgc gcgtgagggc tgcgagctcg tgggcgagca tctccgcatg gcgcgagccc
     1801 gcttcgccga aggtcagggt ccggagcttg atgtgctgag ggctgaggct gccctgtccc
     1861 aggcccaggc ggcgcaggtg ggggcggcgg cggagtcgga cagcgcgcgg cgcgcactgg
     1921 ccgtgcttct cggccgcccc ccttcggaac tggttgaggt cgccgaggct cccgtcggtg
     1981 ccgacgagac ggtagccgac gcggagctcg cctcggccca ggctcgcgag cgggcacccg
     2041 aggtgcgcat cggccaggcc gagctggcgc tcgcccaggc cggcgtcgag gttgcgcgca
     2101 agaccaacaa gcccgatgtg ggcatcgtgc tcagtcacac ccttggcaac gacgcgaccg
     2161 actcacggca cgtgcttcag gctggactgc ggtgggggct ccccttgggc gatcacgggg
     2221 agcggagtgc gcgcgtggat gagggagccg ccagggtgag ggcggccgtc gcgcttctgg
     2281 aggaggccag tcgccaggcc gaggtggccg cccgggatgc tctgggcgct ctggccgccg
     2341 cccggttacg gctggagggg ctgacgcgtg cggccgaggt ggcgcggacg gttcacgagc
     2401 gggccacccg ggggtacgcc gaccgggcca ttgccatgcg agatctgctg gacgcccgcg
     2461 aggacctcac cgccgccgag cgcgaccgcg tgcgggccca gtgcgatgtg gccgtccagg
     2521 aggcacggtt gcgacaggtc ctcggaacgc tggtctcaag ttcgtccgtg gcgacgtcga
     2581 ccgcccacga agcgaccgat cggagaggga gagcctattg agccgcccac gtctcgtcgg
     2641 cctcggcctc ttcatcgccc tgtttgtgcc cctcgcttgg ctggcgttca ggccccgggg
     2701 ctccggagcg gccgatgagg aggaaccaga ggtcgagcca ctg
//
LOCUS       DAOSPL010000539         2736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_37089_length_2736_cov_2.938456, whole genome shotgun sequence.
ACCESSION   DAOSPL010000539 DAOSPL010000000
VERSION     DAOSPL010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37089_length_2736_cov_2.938456"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLQ54_10415"
     CDS             <1..302
                     /locus_tag="PLQ54_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93717.1"
                     /translation="DMGETELAKGENAKVEAVKAGGAKGGSTPSPEPSVSKAAAEPTG
                     ARPKAPAAPSTRPATRRPSPPSRTPPARTTQSTAPKPKPKLPDFPSNPYAQPQQP"
     gene            349..1425
                     /locus_tag="PLQ54_10420"
     CDS             349..1425
                     /locus_tag="PLQ54_10420"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HOS93718.1"
                     /translation="MARLFGRSYSRNELMRRTGNADQFGGVRLSRLEDGKAHDVLVAD
                     FRTGSGLSFTVAPGRGMDIVHSDYCGIPLCWRSSTGDVASEYAEPEEFGWLQGFFGGL
                     VATCGLTYAGAPCVDEGKPLGLHGRVSNIPATNVLADTAWEGDELLMWCQGKVREASV
                     FGPNIVLSRRVSAKLGEKRLFINDVVANEGHETQPHMLIYHINIGYPAVSEDSVFMAP
                     SARQTPRDEPSVRALDRCFGFEAPEDGVPEQLFRHDMVAGPDGRVLTAIVNKTLNFGV
                     YLRHRLDQLPVYSQWKMCASGHYVCGMEPANCEVTGRARARQEGKLQFLQPGEARSYD
                     LELGVVSGADEVAQIESEIRATKG"
     gene            complement(1789..2583)
                     /gene="budA"
                     /locus_tag="PLQ54_10425"
     CDS             complement(1789..2583)
                     /gene="budA"
                     /locus_tag="PLQ54_10425"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HOS93719.1"
                     /translation="MRRHPLSAPLLALLLAVAFWPALGIDDVGPPPDVLFQVSTINAL
                     LDGAYTGGLSIGELRQHGDTGIGTFDCLDGELVLLEGHAYQVLATGEVRSVEDTATTP
                     FASATTFRTDASAELDQPADLDAIKAALDGLRISDNLFYAARVDGHFAAVRTRSVPRQ
                     EKPFPPLAAVTQNQPEFETKDVDGTVVGFWCPRFVEGVNVGGWHLHFLSADRRFGGHL
                     LALAAGRASLAVDAKAQFSLHLPATPEFLQLGIRAGKAAEIDQVEK"
     gene            complement(2636..>2736)
                     /locus_tag="PLQ54_10430"
     CDS             complement(2636..>2736)
                     /locus_tag="PLQ54_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HOS93720.1"
                     /translation="FEIFLTRMALSRKAAKALGCRFEVHINGTRMA"
BASE COUNT          473 a          908 c          946 g          409 t
ORIGIN      
        1 aggacatggg cgaaactgag cttgccaagg gcgaaaacgc caaggtcgag gccgtcaagg
       61 cgggcggcgc caaggggggc agcaccccgt ctcctgagcc ctccgtcagc aaggccgccg
      121 cggagccgac gggcgcgcgg ccgaaggcgc cggcagctcc gtccacgcgg cctgcgactc
      181 gccgccccag cccaccgtct cgcacaccac cggcgcgcac gacgcagagc acggctccga
      241 aacccaagcc gaagttgccg gacttcccga gcaatcccta cgcacagccc caacaaccct
      301 agcgagcccc ctgggggcaa gcgcactcag ctatcggagg acgcaacaat ggcgagactg
      361 ttcggccgtt cctactcccg aaacgagctc atgcgtcgca cgggcaatgc tgaccagttc
      421 ggcggagtgc ggctcagccg cctcgaggac ggcaaggcgc atgatgtgct ggtcgccgac
      481 ttccgcacgg gctctggcct cagtttcacc gtcgccccgg gccgcggcat ggacatcgtg
      541 cacagcgact actgcggcat cccgctgtgc tggcggtcct cgacgggcga tgttgcgagc
      601 gagtacgcag agccagagga gttcgggtgg cttcagggct tcttcggtgg cctcgtggcg
      661 acgtgcggcc tgacctacgc cggcgcaccg tgcgttgatg agggcaagcc gctggggctg
      721 cacggccgcg tgagcaacat cccggccacc aatgtgctcg cagacaccgc atgggagggt
      781 gatgagctgc tgatgtggtg tcagggcaag gtccgtgagg ccagtgtgtt cggccccaat
      841 atcgtgctat cccggcgtgt gagcgccaag ctgggcgaga agcggctgtt cattaacgat
      901 gtcgtggcta acgaggggca tgagacccag ccccacatgc ttatctacca catcaacatc
      961 ggctaccccg cagtcagcga ggactccgtg ttcatggcgc ccagcgccag acagacaccc
     1021 cgcgacgagc cctcagttcg ggcgctcgac cgctgctttg gcttcgaggc gcccgaagac
     1081 ggcgtgcccg agcaactctt ccggcatgac atggtcgcgg ggcccgatgg gcgcgtgctc
     1141 accgctatcg tgaacaagac cctgaacttc ggtgtctacc tgcggcaccg cctcgaccag
     1201 cttcctgtgt actcgcagtg gaagatgtgc gccagcgggc actacgtgtg cggcatggaa
     1261 ccggcgaact gcgaggtcac agggcgcgcc cgggcgcggc aagagggtaa gctgcagttc
     1321 ctccagccgg gcgaggccag gagctacgac ctcgagctcg gcgtggtatc cggcgccgac
     1381 gaggtcgccc agatcgagag cgagatccgg gccaccaagg ggtaggggag cgagagccag
     1441 gcggcttgcg ggcagtctgt gcagagaccg ggggccggtt cgaacggtcc gccgccaagg
     1501 ccagacccgg cccgaggacc tccgccatag cacaaggcca gggtgtcatc ctgggcgtag
     1561 catctgcggc gcgaagggtc tcggctagaa cgagccgggc accgatggca ctggtctgtg
     1621 ccggcgcttc gaggccgctg caggccgaga tccttcgtcg ctggcgctcc tcggaatgac
     1681 acacccggcg gcgcttcgcg ccaggctccc gggatcgccg tggctccgtt ccggccctgg
     1741 gtgcgcaccg gtccccagcc tgcccgtcga gttcccgcgg ccgtgacgct acttctccac
     1801 ctggtcgatc tccgcggcct tgccggcgcg aatgcccagc tggaggaact cgggcgtcgc
     1861 cggcaggtgc aggctgaact gggccttggc gtcgaccgcg aggctcgcgc ggccagcggc
     1921 cagcgccagg agatggccac cgaaccgtcg atctgctgac aggaagtgca agtgccagcc
     1981 gccgacgttc acgccctcga cgaagcgcgg gcaccagaac cccaccaccg tgccatccac
     2041 atccttcgtc tcgaactcgg gctggttctg ggtgacggcc gccagcggcg ggaacggctt
     2101 ctcctggcga ggcacactgc gcgttctcac ggcggcgaag tgcccgtcaa ctcgtgccgc
     2161 gtagaacaag ttgtcggaga tgcgcaggcc atcgagggcc gccttgatag cgtcgaggtc
     2221 agccggctgg tcgagctcgg ccgaggcgtc ggtgcggaac gtcgttgccg aggcgaacgg
     2281 agtggttgcc gtgtcctcca ccgaccggac ctcgccagtg gcgagcacct ggtacgcgtg
     2341 accttccagc aggacgagct cgccatcgag gcaatcgaag gtgccgatac ccgtgtcacc
     2401 gtgttggcgt agctcaccga tggacaggcc gcccgtgtac gcgccgtcca gcagcgcgtt
     2461 gatcgtcgag acctggaaga ggacgtcggg cggggggccg acatcgtcga tgccaagtgc
     2521 aggccagaag gcgacagcga gtaagagggc gagtagcgga gcactgagag ggtggcggcg
     2581 catgtgcggc ctccgttcgc ggcggaaccc gcggcaaccc accggggttg cgcggtcacg
     2641 ccatccgagt gccattgatg tgaacctcga agcggcatcc tagagctttg gcggccttgc
     2701 ggctgagcgc cattcgagtg aggaagatct cgaagt
//
LOCUS       DAOSPL010000540         2734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_37115_length_2734_cov_3.827548, whole genome shotgun sequence.
ACCESSION   DAOSPL010000540 DAOSPL010000000
VERSION     DAOSPL010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37115_length_2734_cov_3.827548"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..>2734)
                     /locus_tag="PLQ54_10435"
     CDS             complement(256..>2734)
                     /locus_tag="PLQ54_10435"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOS93721.1"
                     /translation="VTTAEGRDLLLSSDGARVELGGVATDARGCVLRERHGGVAFAAV
                     VGPGGVWLGGARVLHIDGPEPCAASVERRPGGVCRVTASAPAGSRLSVCVARGTDRAW
                     AMRAGGEPERIPVVREGEEARFTLTAALGPEVVVLLAPAAQPPAPHDAAPALSSLAVD
                     GREVAAAERVDLGRTDPLPGRIEARFASRGRLTTDGVRVTLDGVRLGGECVRVGISEG
                     GRRLLARIGVPADLPPHDHELVVRVDAGELRPLSAQLNLRFSNRPLLTNTGFEDGLRG
                     WSVGSWSGDERTNYRTEAAAENPHSGARCLELRGMVGPLNVVAAQRVELVVGRRYVLT
                     GWYRGDVAAQASLCSQSGAGQYLWSPPIGPSAEWSEFSWEFTVENEERPLLLVCVSAA
                     PAWRASTTSCLRRNGRSPQSRAGRVPRAGEVLRAARIHPHGTKRGEEAHAMRRLELRL
                     VWVLCIVVMALAGVDVQAQSAPGRVHGSLSTIGQGENQIAVLHLWGKPYDMGYAHGKL
                     VPEQVRHLVQGTVQVMAFGAGLSMDQIDAMWKQMEPYVPAEYLEEMRGLADGAGVSYT
                     DVCRMHAVPDISEFHCSFFAAWGKATQDGHLIQIRALDYATMAGIQKYPALVVYEPDD
                     GVPFVNVGWCGFVGMVTGMNAEGIAMSEIGDHLGDEHETLEGEPMPFVMRDVIQHTRS
                     LGEAIYRVRRAPRTSSYLYCIGDAEARDARALVTAKDVFRVYDPDSLPYKRLGQTVYM
                     SMGTDSGWNRKIGDVLARGYGRIDPEFAMIDVMRGLGTGDLHAVAFDVTAGKLWVANA
                     SMKGEDGFGREFVPFCLRECLQSRAGR"
BASE COUNT          481 a         1005 c          849 g          399 t
ORIGIN      
        1 cgtcgagcgt cgagagacct cccgtgtagc taaccgcatg agccgtaccg agggaaccca
       61 ccagcacaag tgcacacagc agtacaacgt aacgtcgcat ctccacccac ctcatcagca
      121 ccccggccgc cacggctggc ggctctcacc cacaccgacc taacaaccat gatacaggaa
      181 tgcgttcccg ttggcactac atttgcactc gctggtggcc tcgcagaccg gccggacggg
      241 gactctccag cctcgtcacc ggcccgcacg cgactgcaag cactcgcgga ggcagaaggg
      301 cacgaactcg cgcccgaacc catcctctcc tttcatggac gcattggcga cccagagctt
      361 cccagctgtg acatcgaatg cgaccgcgtg cagatcgccg gtgccgaggc cccgcatcac
      421 atcgatcatg gcgaattcgg ggtcgatgcg gccgtagccg cgggccagga cgtccccgat
      481 cttcctgttc cagccactgt ccgtacccat ggacatgtat accgtctggc ccagtcgctt
      541 gtagggcagg ctgtccgggt cgtagacgcg gaacacatcc ttggcggtca ccagggcccg
      601 agcgtcgcgg gcctcggcgt cgccgatgca gtagaggtac gagctggttc gcggcgcgcg
      661 gcgaacgcgg taaatggcct cgcccaggct ccgcgtgtgc tggatgacgt cgcgcatcac
      721 gaagggcatg ggttcgccct cgagtgtctc atgctcgtcc cccaggtggt cgccaatctc
      781 gctcatggcg atgccctcgg cgttcatgcc cgtgaccatc ccgacgaagc cacaccaccc
      841 gacgttgacg aagggcaccc cgtcatcggg ctcgtagacc acaagagccg ggtacttctg
      901 gattcctgcc atggtggcat agtccagcgc gcggatctgg atcaggtggc cgtcctgcgt
      961 agccttgccc caggcggcaa agaagctgca gtgaaactcg gagatgtcgg gaaccgcatg
     1021 catccggcac acatccgtgt aggacacgcc ggcgccgtcg gccaggccac gcatctcctc
     1081 gaggtactcg gccgggacat agggttccat ctgtttccac atggcatcga tctggtccat
     1141 cgagagtccg gcgccaaagg ccatgacctg gaccgtgccc tggaccagat ggcgcacctg
     1201 ctccggaacc agttttccgt gggcgtagcc catgtcatac ggcttgcccc acaggtggag
     1261 gaccgcgatc tggttctccc cctggccgat ggtcgagagc gaaccatgga cgcggcctgg
     1321 cgccgattgg gcctgtacgt cgaccccggc cagtgccatc accacgatgc acagcaccca
     1381 taccagtcgg agctcgagtc gtctcatcgc gtgtgcctcc tcgcctcgct tcgtgccgtg
     1441 cggatggatc cgagcggcac gcaggacttc tccggctcgc ggtacgcgcc ctgcccgcga
     1501 ctgcgggcta cgcccgttcc tccgcaagca tgaggtcgtc gaagcacgcc aggccggcgc
     1561 cgctgagacg cagacaagaa gtagcggccg ctcctcattc tcgacggtga actcccagga
     1621 gaactcactc cactcggccg atggcccgat gggcggggac cagagatact gcccggcccc
     1681 gctctgggaa cacaatgacg cctgggccgc cacatcacct cggtaccagc ccgtgagaac
     1741 gtagcgccgg ccgaccacca gctcgacgcg ctgcgcggcc accacgttga gcgggccgac
     1801 catgccccgc aactcgaggc agcgggcccc cgaatgcggg ttctcggccg cagcctcggt
     1861 acggtagttc gtgcgctcat cgccggacca cgagccaacc gaccaccccc ggaggccgtc
     1921 ctcgaacccg gtgttggtga gaagcggccg gtttgagaag cgcaggttca gctgcgccga
     1981 gaggggccgc agctccccgg cgtcgaccct gaccaccagc tcatgatcgt gcggcggcag
     2041 gtcggcgggc acaccgattc gggcgagaag ccggcgcccg ccctctgaga tacccacgcg
     2101 aacacactcg ccgccaagcc gaacaccgtc cagcgtcacg cgcacaccat cggtcgtgag
     2161 ccggccgcga gaggcgaacc gggcctcgat gcggccgggg aggggatccg tacggcccaa
     2221 gtcgacacgc tccgctgccg cgacctcgcg gccatcgacg gcaagcgacg acagcgccgg
     2281 agcggcatca tggggcgcag gaggctgcgc ggccggcgcc agcagcacca cgacctcggg
     2341 ccccagagct gcggtgagtg tgaacctggc ttcctcgccc tctcgcacca cagggatccg
     2401 ctcgggctct ccgcccgcgc gcatcgccca agccctgtcc gttccccggg caacgcagac
     2461 actcagccgc gatcccgccg gggccgaagc cgtgacccgg cagacacccc cagggcggcg
     2521 ctcaaccgag gccgcacacg gctctgggcc gtcgatatgg agcacacggg ctccgccgag
     2581 ccacactccc ccggggccga cgacggcagc gaacgccact ccgccatgcc gctcccggag
     2641 cacgcaccct cgggcgtcgg tcgcgacccc tccgagctcg actcgcgcgc catctgacga
     2701 gagcagcaga tcgcggcctt cggccgttgt gacc
//
LOCUS       DAOSPL010000541         2729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_37205_length_2729_cov_3.285340, whole genome shotgun sequence.
ACCESSION   DAOSPL010000541 DAOSPL010000000
VERSION     DAOSPL010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37205_length_2729_cov_3.285340"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /locus_tag="PLQ54_10440"
     CDS             complement(<1..1343)
                     /locus_tag="PLQ54_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOS93722.1"
                     /translation="MPARHGKAFEIGIRIVADAVMVNAALVVSLDLRFFWDLLMQSHD
                     VGSATARFYDYLGIYLHWFWLLTPVCLVVFYLSGFYTRGRTYRSRYKALIVAQAVVLA
                     YLVAIALGFLVWGGTSIPRGILLVALLTSILFLTGSRVWSSLWRALFRIESRLGHRPP
                     DKGIDSILVIGGAGYIGSSLVPKLLNRGYRVRLLDLFLYGQEPIESFADHPNLEIMRA
                     DFRQVDKVVEAVQDMDAVIHLGAIVGDPACALDSDLTIEVNLMATRMIADVAKGSGVR
                     RFVFASTCSVYGASDQLMDEHSAAKPVSLYARSKLASEQVLMQVSNGDFAPVILRFAT
                     VYGLSGRVRFDLVVNLLTAKAVTEGKITLYGGDQWRPFVHVDDAATAILLALEAPPEV
                     VENRVFNVGADTHNYTLQQVGEMIQTLVPSTELVDTGTDGDRRNYRVCFRRIREAL"
     gene            complement(1351..2586)
                     /locus_tag="PLQ54_10445"
     CDS             complement(1351..2586)
                     /locus_tag="PLQ54_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HOS93723.1"
                     /translation="MAIEGGSPVRHHPLAPWPVFEDDEVAAATAVLRSGKVNYWTGRE
                     GRDFEVEYAASLGRRHAVAMANGTLALEAALFALGVGPGDEVITTPRTFIASASCAVM
                     RGAIPVMADVDPDSGNITAETIRAVLTPRSKAIVAVHLAGWPCDMDPILALAKEHGLW
                     VIEDCAQAHGAVYRGRQVGSMGHAAAFSFCQDKIITTAGEGGMLVTDDQGVWERAWSI
                     KDHGKDYATVYEKDHPPGFRWLHERFGSNWRMPEVQAAVGRVQLPKLPRWVAARRANA
                     KLLQDALSAFPSVRVPVPPADVEHAYYRQYAFVCPERLRSGWDRDRIRDAIVAEGVPC
                     FSGSCSEIYLERAFAAATHPVTGQPLGPAARLPNARMLGETSLAFLVHPTLTADDMAD
                     ACRAVRRVLAAATADTGRR"
BASE COUNT          510 a          934 c          870 g          415 t
ORIGIN      
        1 agggcctccc ggatccgccg gaaacacacc cggtagttgc gccgatcgcc atcggtgccc
       61 gtgtcgacca gttcggtcga cgggacgaga gtctggatca tctcaccgac ctgctgcagc
      121 gtgtagttgt gggtatcggc acccacattg aagacgcgat tctcgacgac ttcgggggga
      181 gcctccagag ccaggagtat ggccgtggcg gcgtcatcga cgtggacgaa gggccgccac
      241 tggtcgcccc cataaagggt gatcttcccc tcggtcaccg ccttggccgt cagcaggttg
      301 accaccaggt cgaagcgcac gcggcccgac agcccgtaca ccgtcgcgaa acgcaggatc
      361 accggggcga aatcaccatt ggagacctgc atgaggacct gctccgacgc cagcttactc
      421 cgtgcgtaca gcgacacggg cttcgcggcc gagtgctcgt ccatgagctg atccgacgcg
      481 ccatacaccg agcacgtgct ggcgaagacg aaccggcgaa ccccgctccc cttggccacg
      541 tcggcgatca tgcgtgttgc catgaggttc acttcaatgg tcagatccga gtccaacgcg
      601 caggccgggt cgccgacgat ggcgcccagg tggataaccg cgtccatgtc ctgaacggcc
      661 tcgaccacct tatccacctg tcggaagtct gcccgcatga tctcgaggtt ggggtgatcg
      721 gcgaacgact cgatgggctc ctgaccgtag aggaacaggt cgagcagccg aacgcggtac
      781 cctcggttca ggagcttggg gacgagcgat gacccaatgt agcccgcccc gcctatcacg
      841 agaatcgagt cgatgccctt gtcgggcgga cggtgcccca gacgggactc gatgcggaac
      901 agtgcacgcc acagcgagga ccagacgcgc gaacccgtca ggaacaggat gctggtcagg
      961 agcgcgacca gcaggatgcc ccgcggaatc gaggttcctc cccacacgag gaaccccagg
     1021 gcgatggcga ccaggtaggc caacacaacg gcctgggcaa cgatcagggc cttgtaccgg
     1081 ctccgatagg tgcggccgcg ggtgtaaaag ccgctcaggt agaacacgac taggcagact
     1141 ggagtgagga gccagaacca gtgtaggtag atgccaagat agtcatagaa ccgggctgtc
     1201 gccgacccga cgtcgtggct ctgcatcaac aggtcccaga agaaccgcag gtcaagcgag
     1261 accaccagcg ccgcgttgac catcaccgcg tcggccacga tgcggatgcc gatctcgaac
     1321 gccttcccgt gacgcgcggg caccctcaag tcagcgcctc cccgtgtcgg ctgttgccgc
     1381 cgcgagtacc ctgcggaccg cccgacaggc gtcggccatg tcgtcggccg tcaacgttgg
     1441 gtggacgagg aacgcaaggc tggtctcccc gagcatgcgg gcgttcggca gccgggctgc
     1501 cggccccaac ggctgacccg tgaccggatg cgttgccgcg gcgaaggcgc gttccaggta
     1561 gatctcgctg caactgccgc tgaaacacgg cacgccctcc gcgacgatgg cgtcccgaat
     1621 ccggtcgcgg tcccaccctg agcgcaggcg ctcggggcac acgaaggcat actgccggta
     1681 gtaggcgtgc tcgacatccg ccggtggcac ggggacacga accgacggga acgccgacag
     1741 cgcgtcctga agcaacttgg cgttggcccg gcgcgcggct acccatcgcg gcagcttcgg
     1801 aagctgcacc cggccgaccg ccgcctgcac ctccggcatc cgccagttcg atccgaagcg
     1861 ttcatggagc caccggaatc cgggcgggtg gtccttctca tagaccgtcg cgtagtcctt
     1921 gccgtggtcc ttgatgctcc aggcgcgctc ccagaccccc tggtcgtcgg tcaccagcat
     1981 gccgccctcg cccgccgtgg tgatgatctt gtcctgacag aacgagaacg ctgccgcatg
     2041 gcccatggag cccacctgac gacctcggta gactgcgccg tgggcctggg cgcagtcctc
     2101 gatcacccac aaaccgtgct ccttggcaag tgccagaatg gggtccatat cgcacggcca
     2161 tccggccaga tggacggcca cgatggcttt gctgcgcggg gtcagcacgg cgcgaatggt
     2221 ctcggcagtg atattccccg agtcggggtc aacgtcggcc atgacgggaa tggcgccgcg
     2281 cattacggca cagctcgccg acgcgatgaa cgttcgaggc gtcgtgatga cctcgtcgcc
     2341 gggaccgacg cccaaggcga acagcgcggc ctcgagcgcc aaggtgccgt tggccatggc
     2401 cacggcgtgc cggcggccta gcgatgccgc gtactcaacc tcgaaatcac ggccctcgcg
     2461 tccggtccag tagttgacct tcccggagcg caggactgcc gttgccgcag cgacctcgtc
     2521 atcctcaaac acgggccacg gcgccagcgg gtggtggcgt accggcgaac caccctcgat
     2581 ggccagaggc ccttggccct cttcacactg gctagcaacc aaggcgaacc acttctcccg
     2641 acagcccggg agcaacccct ccggacagca gtatggcctc aggcacccga cgccagaggc
     2701 ccgtccgttc cctggaactc ctcgacagt
//
LOCUS       DAOSPL010000542         2728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_37226_length_2728_cov_3.217733, whole genome shotgun sequence.
ACCESSION   DAOSPL010000542 DAOSPL010000000
VERSION     DAOSPL010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37226_length_2728_cov_3.217733"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1723
                     /locus_tag="PLQ54_10450"
     CDS             <1..1723
                     /locus_tag="PLQ54_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS93724.1"
                     /translation="GLSGRQISGSCVCLAIVAVAGLVPPLVIGRVIDDLGAGRFGRTG
                     TYVLMLLGLFAAEGLFSGIRNRLMHVAGEWFVLRIRSDAYRSVQRLSLSYFEQNTTGD
                     IMSRLSGDVDAIEDMLVHGTDDILVNAIRFVGITGMLIYLDWRIALAVALPAPFIALG
                     LFTFAKRIRKTYRLVRDQLGDLNAKLQDNITGIRVIKGFAAEGREFELFEGKSRDYAN
                     RRIHLIRLWTVFYPVMEFLGGLGFIAVIWVGSRLIQSGEFSAGDLVATFAYVMQFYGP
                     IHTFSRINETIQRALAAADRVFELMDAQPDVVDREGAHELDRVRGKVEFRNVGFRYAS
                     GGEVLRDVSFVAESGECVALVGRSGAGKTSVINLIPRFYDPIEGQVLVDDIDVRDLVS
                     ASLRRNVAMVLQDTFLFNGTVRENIAYARPGASDAEIESAARIANAHEFIEGLENGYD
                     TEIGERGVKLSGGQKQRLSIARAVLADPRILILDEATSSVDTESELLIHEALEKLIRG
                     RTTFIIAHRLSTVQKADKIIVLEEGRIVEEGNHQSLLAKGGAYARMCEMQFALAEVER
                     DYAAVST"
     gene            1741..>2728
                     /locus_tag="PLQ54_10455"
     CDS             1741..>2728
                     /locus_tag="PLQ54_10455"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOS93725.1"
                     /translation="MVDSRWRALVMVAAAVLLCVVSGCIGAVGGGTPPAVDITATTGK
                     VEGFIYITPAQAGSAGAGRQAITTRFVYSASENAELGQPAADGTRVSAPGGTGTTTGG
                     FFSLPAVQVGLRTLTVTGPIDGQEATQELPGFLVVPDDVQGLGISVVVLPAALTEVPA
                     GGTTQLTAELRAGSYHVAAYPASDFVWSSQSEAIATVDGSGTVAGVASGQARITAQTG
                     PYAGSRTVSVEGNVRHEVGQLWLGALTSYLDNGHQGYNFREAALVSDPSASRGTGDMH
                     MDVLGQVHAWGTGALSLGTSVALDASIVPPAEGYVTETLQLQVGEVAVIKLRD"
BASE COUNT          442 a          779 c          988 g          519 t
ORIGIN      
        1 tgggctttca ggcaggcaaa tatccgggtc ttgcgtgtgc ctggccattg tggccgtggc
       61 ggggctggtt ccaccactgg tcattggccg ggtgatcgat gacctcgggg ccggccggtt
      121 cggccgtacc ggcacgtatg ttctgatgct cttgggtctg ttcgcggccg aagggctgtt
      181 cagtgggatc cgcaaccgcc tcatgcacgt ggccggcgag tggttcgtgt tgcgcattcg
      241 ttcggatgcg taccggtcgg tgcaacggct atcgctttcc tacttcgagc agaacaccac
      301 gggtgacatc atgtcacgcc tgtcgggcga cgtggacgcc atcgaggaca tgctcgttca
      361 cggcaccgat gacatcctgg tgaacgcgat tcgctttgtg ggcatcacgg gcatgctgat
      421 ctacctggac tggcggattg cattggctgt ggcgctgccg gcccctttca tcgcactggg
      481 cctgttcaca tttgccaagc gcatacgaaa gacatatcgc ctggtgcggg atcagctcgg
      541 cgacctcaac gcgaagctgc aggacaacat caccgggatc cgcgtgatca aggggttcgc
      601 ggccgagggg cgcgagttcg agttgttcga gggcaagagc cgcgactacg ccaatcgacg
      661 tatccacctg atccgtctgt ggacggtgtt ctaccccgtg atggagttcc tcggcggcct
      721 gggtttcatc gccgtgatct gggtggggtc tcggctgatc cagtcgggcg agttctcggc
      781 gggcgacctg gtggcgacgt tcgcctacgt gatgcagttc tatggtccga ttcacacgtt
      841 ctctcggatc aatgagacga tccagcgggc cctggcggcc gcggatcggg tcttcgagct
      901 catggacgcg cagccagacg tggtagaccg cgagggagcc cacgagctgg atcgcgtgcg
      961 gggcaaggtg gagttccgga acgtagggtt ccgctatgcg tcgggcggcg aggtgctccg
     1021 cgatgtctcg ttcgtggccg agagtggcga gtgtgtggcg ctcgtgggcc ggagtggcgc
     1081 cgggaaaacg agtgtcatca acctgattcc tcgcttctat gatcctatcg agggccaggt
     1141 gctggtggat gacatcgatg ttcgtgacct ggtgagcgcg tccttgcggc gcaatgtggc
     1201 catggtcttg caggatacgt ttctcttcaa cggcacggtg cgggagaaca tcgcatatgc
     1261 tcggcccggg gccagtgacg ccgagatcga gtccgccgcc cggattgcca acgcgcacga
     1321 gttcatcgag gggcttgaga atggctacga cacggagatt ggcgagcgcg gtgtgaagct
     1381 atcgggaggc cagaagcagc ggctatccat cgcccgggcg gttttggccg acccgcgcat
     1441 tctgattctc gacgaggcga catcgtccgt ggacacggag tcggagctgc tgatccacga
     1501 agcgctggag aagctcatcc ggggccggac gacgttcatc atcgcgcatc ggctttccac
     1561 cgtgcagaag gccgacaaga tcatcgtgct ggaggagggt cgcatcgtcg aggaaggcaa
     1621 tcatcagtct ctgctggcta agggcggcgc ctatgcccgc atgtgcgaga tgcagttcgc
     1681 gttggctgag gtggagcgcg actacgcggc cgtgtccacg tagcagaggg cgggtggcag
     1741 atggtagaca gcagatggcg agcattggtg atggtggcag ccgccgttct gttgtgcgtg
     1801 gtgtctgggt gtattggagc cgtgggcgga ggcacgcccc ccgctgtcga catcacggcg
     1861 acgaccggga aggtcgaggg cttcatctac atcacaccgg cccaggcggg ttcggccgga
     1921 gccggccggc aggccatcac gactcggttc gtgtactccg ccagcgagaa cgcggagctg
     1981 ggtcagccgg cggccgacgg cactcgagtg agcgctccag gtggcacggg aaccaccact
     2041 ggcgggttct tctcgctgcc ggccgtgcag gtgggattgc gcacactcac agtgacgggg
     2101 ccgattgacg ggcaggaggc gacgcaggag ctgcccgggt tcctggttgt gcccgacgac
     2161 gtgcaggggt tggggatctc ggtggtcgta ctcccggccg ccctgacaga ggttccggcg
     2221 ggcggaacca cgcagcttac ggcggagctc agggcaggga gctaccacgt ggcggcctac
     2281 cctgccagcg acttcgtttg gtcgtcgcaa tcggaggcta tcgccacggt agatgggagc
     2341 ggcaccgtgg cgggagtggc ctcgggccag gcccggatca ccgcgcagac cggaccgtat
     2401 gccgggtcga gaaccgtgtc ggtcgagggc aacgtgcggc acgaggtggg ccagttgtgg
     2461 ctgggggcgc tcacttccta tctggataac ggccaccagg ggtacaactt ccgcgaggcg
     2521 gcgctggtta gcgaccccag cgcgagccga ggcacgggcg acatgcatat ggacgtgctg
     2581 ggccaggttc atgcgtgggg cacgggcgcc ctgagcctgg ggacctcggt ggcgctggat
     2641 gcttccatcg tgccgccggc ggagggttac gtcacggaga ctctgcagct gcaggtgggc
     2701 gaagttgccg tcatcaagct ccgcgacg
//
LOCUS       DAOSPL010000543         2726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_37256_length_2726_cov_2.670910, whole genome shotgun sequence.
ACCESSION   DAOSPL010000543 DAOSPL010000000
VERSION     DAOSPL010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37256_length_2726_cov_2.670910"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2726)
                     /locus_tag="PLQ54_10460"
     CDS             complement(<1..>2726)
                     /locus_tag="PLQ54_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOS93726.1"
                     /translation="GSLADRAQQVADAGLHLVPTIGRKPGLPSGLVDADTRAAAEAVA
                     AGESATLGLRLGSATRTTDDDGDSRWTMALDFDAVTGQWCGWSENGRELLSPSTRPDV
                     TVAGPELEWPAPGLWQADEPVAIVEQGVYRVSVRRHAGDWTLTTQFEAYPDERRLVRS
                     VTLRWNGAATLRTHQVTLRVPGLSLGDSQSAVGCLPGAYPPHEFPLAIDGPSRAELEP
                     GWTWSETGCAYVRSDAAGLSVLGAYRLEDDRARVSLETGQGGPALVHHFEVVARLAPG
                     SEITVGSQCLQLAEPDLPAIREAGASLSERVNFGPPPDRPQWLDGAVLYEVHPWGRLE
                     AWGAGDRGDRFPSLETQLPYLKSLGVSALWLLPVTEKPPWVYYLPKLREIDPDVGTLA
                     ELASMIRAGHGLGIRTLMDIVTYGVAPDSPDVAHLPDTVWCLDENGERQKAWGGTVMA
                     ADCSNSDWNAHIVDLGSYWVRETGCDGYRLDCGGAGQVPNYSPKAHPKANRAMLAGGV
                     RQNALLRAAIRAINPDAVLLPEAGATGNFRSADMLFDYPFYMACRELTREPDTGLAVT
                     RLRDWLAAQQITHSLRQQLSMVRFLELHDTVSAQEFFGLGPSQALTALSTFIPGVLLL
                     QQEQEIGMAEELRQWLQLRHELPELGRGEADYRGVTVDDPRVLAFTRRSVDGASIVLV
                     NFAPVALSCHVRWQEDLTEVFPEATDALTGAAVADGQPITVPAYRPIVIALRRDGAGP
                     RPRIPSREATPGPLRAAAPEVAPLGDGLERHTVRVTGATEWFVLTTEGFAMDEFVDRH
                     RGTKPGETHVDAVPPLARVWRPLENRLWDGPGPTGFGLAGAAGDAVWLPITDREALRG
                     ARIEADSPAGGDVRLVIDALAGAQPFEIAETTRDELLSMARVARPPASAARV"
BASE COUNT          392 a          919 c         1025 g          390 t
ORIGIN      
        1 tcgacgcgcg cggccgatgc ggggggtcgt gcgactcgcg ccatgctcag cagctcgtcg
       61 cgggtcgtct cggcgatctc gaagggttgg gcccctgcga gggcgtcgat caccaggcgg
      121 acgtcgccgc cggccggcga gtcagcctcg atccgggcgc cgcggagtgc ctcgcgatcg
      181 gtgatgggga gccacacggc gtctccggcg gctccggcca ggccaaaccc ggtggggccg
      241 gggccgtccc agaggcggtt ctcgagtggc cgccacacgc gggccaaagg cggcacggca
      301 tcgacgtgcg tctcgcccgg cttcgtgccc cgatggcgat cgacgaactc gtccatggcg
      361 aacccctcgg tggtgaggac gaaccattcg gtggcgccgg tcacgcggac ggtatgacgt
      421 tcgaggccat cgccgagggg agcgacttcg ggcgcggcgg cccggagggg gccgggcgtc
      481 gcttcgcgcg aggggatgcg aggtcgcggc ccggctccgt cccgccggag cgcgatgacg
      541 atcgggcggt acgcgggcac ggtgatcggt tgcccatccg cgacggccgc gccggtgagt
      601 gcatcggtgg cctcggggaa cacctcggtg aggtcctcct gccagcggac gtggcacgac
      661 agcgccaccg gggcgaagtt gaccaggacg atggaggccc cgtcgaccga ccgccgcgtg
      721 aaggcgagca cgcgcgggtc gtcgacggtg acgccccggt agtcggcctc gcccctgccg
      781 agctcgggga gttcgtgccg gagctgcagc cactggcgaa gctcctcggc catgccgatc
      841 tcctgctcct gctggaggag gagcaccccg gggatgaagg tgctcagcgc ggtcagggcc
      901 tgcgatgggc cgagaccaaa gaactcctgc gccgagacgg tgtcgtgcag ctccagaaaa
      961 cgcaccatcg agagttgctg gcgcagcgag tgggtgatct gctgcgcggc gagccagtcg
     1021 cgaagccggg tgacggcgag gccggtgtcg ggctcccgtg tcagctcgcg acacgccata
     1081 tagaaggggt agtcgaacag catgtcggcc gagcggaagt tcccggtagc gcccgcctcg
     1141 ggcaagagga ccgcatcggg gttgatggcg cggatcgcgg cccgcagtag ggcgttctgc
     1201 cggaccccgc cggcgagcat ggcccggttg gctttggggt gggctttcgg gctgtagttg
     1261 ggcacctggc cggcgccacc acagtcgagc cggtagccat cgcagcccgt ctcgcgcacc
     1321 cagtaggagc ccaggtcgac gatgtgggca ttccagtccg agttggagca gtccgcggcc
     1381 atgacggtac caccccaggc cttctggcgc tcgccattct catcgagaca ccacacggtg
     1441 tcgggcagat gggccacgtc ggggctatcg ggcgcgacgc cgtaggtgac gatgtccatg
     1501 agcgtgcgta tgccgaggcc gtggccggcg cggatcatgg aggcgagctc ggcgagcgtg
     1561 cccacgtcgg ggtcgatctc ccgcagcttc ggcaggtagt agacccacgg gggcttctcg
     1621 gtcacgggca ggagccagag ggcgctgacg cccaggctct tcaggtatgg caactgggtt
     1681 tcgagactcg ggaaccggtc gccgcggtcg ccggcgcccc aggcctcgag tcggccccag
     1741 gggtggacct cgtagagcac ggctccgtcg agccattggg gccggtcagg cggcggtccg
     1801 aagttgaccc gttccgacag gcttgccccc gcctcgcgga tcgctggcag gtctggctcc
     1861 gccagctgca gacactggga gccgaccgtg atctcgctgc ccggcgcgag ccgggccacc
     1921 acctcgaaat ggtgcacgag cgccggcccg ccctggcccg tctcgaggct cacgcgcgcg
     1981 cggtcgtcct cgagccggta agcgccgagc accgaaaggc cggcggcgtc cgagcgcacg
     2041 taggcgcagc cggtctcaga ccacgtccac cccggttcga gttcggcccg cgacggcccg
     2101 tcgattgcca gagggaactc gtggggtggg taggcgccgg gtaggcagcc gacggcggat
     2161 tgcgagtcac ccaggctgag gccaggcacg cgcaaggtta cctggtgggt gcgaagcgtg
     2221 gccgccccat tccagcggag tgtgacgctt cggaccaggc gcctctcgtc cgggtatgcc
     2281 tcgaactggg tggtcagcgt ccagtctccg gcgtgccggc ggacggagac gcggtatacg
     2341 ccctgctcca cgatggcgac gggctcatcg gcctgccaca agccgggtgc cggccattcg
     2401 agttccgggc cggccacggt aacatcaggt cgagttgagg gagacaggag ctcccggccg
     2461 ttctccgacc agccgcacca ctgacccgtg acggcgtcga agtccagcgc catcgtccag
     2521 cggctgtcgc cgtcgtcgtc ggtcgtccgg gtggcggacc cgagcctgag cccgagcgtc
     2581 gccgactccc cggcagctac ggcctcggcc gccgcccggg tgtcggcgtc cacaagccca
     2641 gacgggagtc cgggcttgcg gccgatcgtc ggtaccaggt ggagtccggc gtcagcgacc
     2701 tgctgcgccc ggtcagcgag cgagcc
//
LOCUS       DAOSPL010000544         2724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_37285_length_2724_cov_3.811915, whole genome shotgun sequence.
ACCESSION   DAOSPL010000544 DAOSPL010000000
VERSION     DAOSPL010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37285_length_2724_cov_3.811915"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PLQ54_10465"
     CDS             complement(<1..563)
                     /locus_tag="PLQ54_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93727.1"
                     /translation="MKARSLWPMVCPCVILLAAAARPEADATTDNPVVGPPAAPTDTP
                     TAPDKGPLITITPEGVSVYAVRCDAHALLTDIARKTGFSIIVDDSVRGVLTVNFKNRK
                     VSDIIRDIAATYGLACREENGVFTITEGLPAHVSSYLLSEMASVRPDYIPASDAEQLL
                     PVFLQDYTAPSLHQNAVVLSAPPEILE"
     gene            870..2339
                     /locus_tag="PLQ54_10470"
     CDS             870..2339
                     /locus_tag="PLQ54_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93728.1"
                     /translation="MDSELAATEDWVRGENLDTDTTEALADLPRLLADYSAAEGADRA
                     SLGQRLTTRFFTLYAGAGARACAEGPDARDLRLLRCAAPVAGLLGDEMASVVDSLCAD
                     PGPVGPVYYVDEWLMAVFRQEVAPSTTDEVSTMSARPSQSVSAQLARAESEVASLKPQ
                     AVQALKSSGSADDALLECAQRIVALGQDLRKLLAEGAEGQMPSAAPLQRERARIAQEL
                     RSQIENLSGNDRGTVEVCSKYAEAVSLRRRLQARMAVDGAEGVADSSALGTELATIRQ
                     MMKMAIGPRGNHFPIASPAFVRTTLAEGVNSRASVIEKIQQVEAIEPGLFRRVSFGQE
                     IRIEPLVLLLPCSGSHGICWEAWQKGNRRTPGRIAVPLYCGRPQNDLVVRALGAHRWL
                     VAQETAMHYWMEEGLTGAYYQFRGKSAGSRIDEAFAQDYVSWITREAQGRPVLDAKLR
                     ALFWHHLPFSPEVKARLKGIGAYRSLLEADARKAAAAAV"
     gene            complement(2351..>2724)
                     /locus_tag="PLQ54_10475"
     CDS             complement(2351..>2724)
                     /locus_tag="PLQ54_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93729.1"
                     /translation="PPAYDGGRTAGPLPPPPFPGRVVSVGSCPYYQYGGSGVVELRET
                     ADGLSLTIYPDVDRVGEGWSGTLERPLTRLVEREHDFRLTRPGWTRARVERRQGGSWV
                     PVAGDGTGFAARPGEYRLLRR"
BASE COUNT          436 a          884 c          957 g          447 t
ORIGIN      
        1 ttctcgagga tctcgggcgg ggcggacagc accacggcgt tctgatggag acttggcgcc
       61 gtatagtcct gcaggaacac ggggaggagc tgctcggcat cgctggccgg tatgtagtcg
      121 ggacgcaccg aagccatctc cgagagcagg taggacgaga cgtgggccgg cagtccttcg
      181 gtgatggtga acacgccgtt ctcctcgcgg caggccaggc cgtaggtggc ggcgatgtcc
      241 cggatgatgt cggacacctt gcggtttttg aagttcacgg tcaggacgcc acggaccgag
      301 tcgtcgacga tgatcgagaa cccggtcttc cgggcgatgt cggtcagcag ggcatgcgcg
      361 tcacaccgga cggcatagac ggacacccct tcgggggtga tggtgatgag gggcccctta
      421 tcgggtgccg tgggtgtgtc ggttggggcg gcgggcgggc cgaccacagg gttatcggtg
      481 gtggcgtccg cctcgggacg ggctgccgcc gccaggagga tcacgcaggg gcagaccatg
      541 ggccacaatg atcgcgcttt cacaccgggg acctcctcag ggctcgcaca cggccgactg
      601 cggggggact ccttcgttac ggcggcccga cggccctggg tgcgggtcgg gtcacgaccc
      661 cagggcattg ccgacggcgc catgggaagc cggataaccc tacaccttcg ttggcgcccc
      721 cccttgacct cctcgtgaag cgtcccgaaa ctgtcacgcc ttgttgaccg aactggctcg
      781 gaaccgggcc gctgtccacc gccgccgtgt ggaaccctgt gcatggctgc cgcgcgtcgc
      841 tctggcttgc catggggtgg gtgatcgtat tggatagtga acttgcagcc accgaggact
      901 gggtacgcgg tgaaaacctg gataccgata ccactgaggc tctggccgac cttccgcgtc
      961 tcctggccga ctactccgcc gccgagggcg cagaccgcgc ctcgcttggc cagcggctga
     1021 ccacgcggtt cttcacactg tacgctggag ccggcgcgcg cgcctgcgcg gaggggcccg
     1081 atgctcgcga cctcagactg cttcgctgtg cggcccccgt ggcggggctt ctcggcgatg
     1141 agatggcctc agtggttgac tcgctttgcg ccgacccggg accggtgggg ccggtctact
     1201 atgtggacga atggttgatg gccgttttcc gccaggaggt tgcgccgagt accactgacg
     1261 aggtcagcac catgtcggcc cgccccagcc agtcggtatc ggctcagctg gcccgggccg
     1321 aatccgaagt ggccagcctg aagccccagg cggtgcaggc gctgaagtca tcgggcagcg
     1381 cggacgatgc cctcctcgaa tgcgcacaac gcatcgtggc actggggcag gatctacgca
     1441 aactgctcgc cgagggcgct gagggacaga tgccctcggc ggctccacta cagcgcgagc
     1501 gcgcccggat tgcccaggag ctgcggagcc agatcgagaa cctgtcgggt aacgaccgcg
     1561 gcacggtcga ggtctgctcc aagtatgccg aagccgtctc tttgagacgg cgtctgcagg
     1621 cacgaatggc cgtggacggt gccgaagggg tggcggactc ttcggctctg ggaaccgaac
     1681 tggcgaccat ccggcagatg atgaagatgg ccattgggcc aagagggaac cacttcccca
     1741 tcgccagccc agccttcgtg cgcaccaccc tggcagaggg cgtcaactcg cgggcgtcgg
     1801 tcatcgagaa gattcagcag gtcgaggcca tcgaacccgg cctgttccgg cgggtctcat
     1861 ttggccagga gatccgcatc gagcccctgg ttctgctgct accgtgctcc ggcagtcacg
     1921 gcatctgttg ggaagcctgg cagaagggca accggagaac acccggtcgc atcgccgtcc
     1981 cactctactg tggccgcccg cagaacgacc tggtcgttcg ggcccttggt gcccaccgct
     2041 ggctggtcgc tcaggagacg gccatgcact actggatgga ggagggcctg accggagcct
     2101 actaccagtt tcgcgggaag tcggctggct ccagaatcga cgaggccttc gcgcaggact
     2161 acgtgagttg gatcacccgc gaggcgcagg gccgtccggt gctcgacgcc aagctgcgcg
     2221 ctctgttctg gcaccacctg cccttcagcc ctgaggtgaa ggcgagactc aagggcatcg
     2281 gcgcctaccg ctcgctgctc gaggccgacg cgcggaaggc agcggcggcg gccgtgtagt
     2341 ttcgggcggg tcaccgccgg agcagccggt actcgccggg gcgggcggcg aatcccgtgc
     2401 cgtcgccggc gaccgggacc caggagccgc cttgccgtcg ctcgacgcgc gcccgagtcc
     2461 agccgggccg cgtgagccga aagtcgtgct cgcgttcgac gaggcgcgtg agcggtctct
     2521 cgagtgtgcc gctccagccc tcgcccaccc ggtcgacgtc ggggtagatg gtcagcgaaa
     2581 ggccgtccgc agtctcacgc agctctacca ctcccgagcc gccatactgg tagtatggac
     2641 aggacccgac cgagaccacc cggccaggga acggtggagg cggcagtggc cctgcagttc
     2701 ggccgccgtc gtatgccggg ggtc
//
LOCUS       DAOSPL010000545         2722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_37326_length_2722_cov_3.054743, whole genome shotgun sequence.
ACCESSION   DAOSPL010000545 DAOSPL010000000
VERSION     DAOSPL010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37326_length_2722_cov_3.054743"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLQ54_10480"
     CDS             <1..513
                     /locus_tag="PLQ54_10480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93730.1"
                     /translation="LSYVANELLLPRKKYAAVPQQCVSLSQVDAPAEVIMVAEYTNKI
                     QCLLDSSPTGGDAIKSHRPTNAVSMGGAVFDGESWAGQPVIALTPQQAWQAIAQAEAS
                     SAMGNHHICYINPQAHNGGSNYIFADGHAKWYLLDKTLDPTHFLWGKKAYSCPGSPPV
                     YNPLTGTPVS"
     gene            653..1981
                     /locus_tag="PLQ54_10485"
     CDS             653..1981
                     /locus_tag="PLQ54_10485"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOS93731.1"
                     /translation="MGLKACKFGGTSLADANGVRRVAEIVAREPARRYVVVSAPGKRE
                     PSDHKVTDLLLASHSLARQGLDFTQPLGMVRDRFRSLAADLGVRLDVRPLLDELEAQV
                     RTEGNRDLIASRGEYLCARIVADFLGARFVDARRAIGVARGRSQVTPDTYPRLRMLLE
                     GEGPVVVPGFYGSDTHGDIVTFPRGGSDITGSVVARAVGAEVYENWTDVPGLLMTDPA
                     VVPEAQPILEVTYRELRELSYMGAKVLHEEATFPVREAGIPIHIRSTWEPDAPGTRVL
                     PSREHGTAPVVGIAGRTGCSTIHIEKAMMNEGRGFGRRVLDILEFHGISWLHAPTGID
                     TMSVVVWDEELEGKGDAYLADIERILEPDRLELIGDLALLATVGQGMANTVGIAGRLF
                     TSLADAGVNIRMIDQGSSEISIIVAVAAADYSKAVRAIYDAFAGPCRSRA"
     gene            2037..>2722
                     /locus_tag="PLQ54_10490"
     CDS             2037..>2722
                     /locus_tag="PLQ54_10490"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS93732.1"
                     /translation="MTRVVPMLAALAGCALALAMGFSCRVPLEAQPGASEPAAQTPVA
                     EVSQGTSSEVDQFREWVAQAPTLPSLRLTLARHYERTFEGKGDEAARSQLLTALCLQP
                     DDPDALFELGQVLFRLKRYRAAAGWLERAVAAEPDSWVRLHWLALAHIRSGRPEEAVE
                     PLRQATRLAPEERDPRRRAADVFSRQGLTQEARVHLRWLVRNAKAQEDRQWAETELML
                     LDMSERRYWG"
BASE COUNT          443 a          904 c          933 g          442 t
ORIGIN      
        1 ctgagctacg tcgccaacga gctgttgctt cctcggaaga agtacgcggc agtgccccag
       61 cagtgcgtgt ccctgtcgca ggtggacgct ccggccgaag tgatcatggt cgccgagtac
      121 accaacaaga tccagtgcct gctggacagt tctcccacgg gtggcgacgc catcaagtcg
      181 caccggccga ccaatgcggt gagcatgggt ggagccgtgt tcgacggcga gagctgggcc
      241 ggccagccgg tcatcgccct gacgccacaa caggcctggc aggccatcgc tcaggctgag
      301 gcgagtagcg ccatgggcaa ccatcacatc tgctacatca acccccaggc gcacaacggc
      361 ggcagcaact acatcttcgc ggatggtcat gcgaagtggt atctgctgga caagacgctg
      421 gatccgaccc atttcctgtg gggcaagaag gcgtactcgt gccccggctc gccgcccgtg
      481 tacaaccccc tgacgggcac gcccgtgagt tgatccccgc ataccccggc catcgcggac
      541 gttgcccggc gcggcgcccg cgggagcact ggctcccgcg ggcgctttcc gcgtaccata
      601 gcaggaactc ggcgcggaag accgtacgta cggacaggga aggtggcagc ggttgggtct
      661 gaaggcatgc aagtttggtg gcacatcgct ggcagacgcc aacggcgtcc gccgggtcgc
      721 cgagatcgtc gcccgggaac cggcgcgacg atacgtggtg gtctcggcac ccgggaagcg
      781 tgagccatcg gatcacaagg ttaccgacct gctgctcgcc tcccactcat tggctcggca
      841 gggcctcgac ttcacacagc cgctgggaat ggtccgtgac cgcttccggt cgctcgcggc
      901 cgatctcggg gtccggcttg acgtgcgtcc gctgctcgac gaactcgaag ctcaggtgcg
      961 caccgagggc aaccgagatc tgattgcctc gcgaggcgag tatctgtgcg cgcgcatcgt
     1021 ggccgacttc ctcggggcac gctttgtcga tgcccggcgt gccatcggcg tggcacgagg
     1081 ccgttcgcaa gtcacccccg acacctatcc gcgcctgcgc atgctgctcg agggcgaggg
     1141 cccggtggtc gtgcccgggt tctacgggtc ggacacccac ggtgacattg tcaccttccc
     1201 acggggtggg tctgacatca ccggctctgt ggtggcgcga gcggtggggg ctgaggtcta
     1261 cgagaactgg accgatgttc cgggactgct gatgaccgac ccggccgtgg tccccgaagc
     1321 ccagcccatc ctcgaagtga cctaccggga gctccgcgag ctgtcgtata tgggcgccaa
     1381 ggtcctgcac gaggaggcca cgttccccgt tcgcgaggcc ggcatcccga tccacatccg
     1441 gagcacgtgg gagcccgatg cgccagggac gcgagtgctg ccatcgcgag agcacggcac
     1501 ggcccccgtc gtcggcattg ccggccgaac cggctgttcg accatccaca tcgagaaggc
     1561 catgatgaac gaggggcgcg gcttcgggcg ccgtgtgctc gacatactgg agttccacgg
     1621 catctcctgg cttcacgcac ccaccggcat tgacaccatg tcggtggtgg tctgggacga
     1681 ggaactcgag ggcaagggcg atgcgtacct ggccgacatc gagcgcattc tggaaccgga
     1741 ccgactggag ctgatcggcg atctggccct ccttgcgacg gtggggcagg ggatggcgaa
     1801 caccgtcggt atcgctggac gtctgttcac ctcgctggct gatgcgggag tgaacatccg
     1861 catgatcgac cagggctcct cggagatcag catcatcgtg gcggtcgccg ccgccgacta
     1921 ctcgaaagcg gtgcgcgcca tctacgacgc attcgcgggc ccgtgccgct cgcgggcata
     1981 ggcccggacg tctcgggctc accccgaggc tcgctgggga cggagatgcg ctgacgatga
     2041 ccagggtcgt tcccatgctg gccgccctag ccggctgcgc tctggccttg gccatggggt
     2101 tctcgtgccg ggttccgttg gaggcgcagc cgggcgcgag cgagccggct gcccagacgc
     2161 cggtggccga ggtctcgcag ggcacctcat ccgaggtcga ccagttccgc gagtgggtgg
     2221 cgcaggcacc gacgttaccg tccctgaggt tgaccctggc ccggcactac gagcggacgt
     2281 tcgagggaaa gggcgacgag gcggcgcgca gccagcttct gacagccctc tgtctgcagc
     2341 ccgacgaccc cgacgcgctc tttgaactgg gacaagtgct cttccgcctg aagcgctacc
     2401 gcgcggcggc tggttggctc gagcgagccg ttgcggccga gcccgatagc tgggtgcggc
     2461 tccattggct ggcgctggcc cacatccgga gcggccggcc cgaggaagcg gtcgaaccgc
     2521 tgcgccaagc cactcgcctg gcccctgagg agcgcgaccc gcgtcggcgc gcggccgatg
     2581 tcttctcgcg gcaggggctc acccaagagg ctcgtgtcca tctgcgatgg cttgtgcgga
     2641 acgccaaagc ccaggaagac cgccagtggg ccgagaccga gctgatgctg ctcgacatgt
     2701 cggaacggcg ttactggggc ca
//
LOCUS       DAOSPL010000546         2718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_37413_length_2718_cov_2.858430, whole genome shotgun sequence.
ACCESSION   DAOSPL010000546 DAOSPL010000000
VERSION     DAOSPL010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37413_length_2718_cov_2.858430"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PLQ54_10495"
     CDS             complement(<1..502)
                     /locus_tag="PLQ54_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93733.1"
                     /translation="MAQSDLPEVERTAQRVRLVYRPIVGHVQDFHLSLAGSLVYNELN
                     DFALRAEADFRVAVLGIDAETATLGLHVRSGFYEVLGSRTGLDCVGETLVVKVGRDHC
                     IREVVVDPRLAAKRDELDLRTILIHLSTLVDFPNKGLKAGSTWSGDIASVDARGNAIR
                     LLTRNEC"
     gene            complement(637..2190)
                     /locus_tag="PLQ54_10500"
     CDS             complement(637..2190)
                     /locus_tag="PLQ54_10500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOS93734.1"
                     /translation="MPATEIPQFVAPRFETADWAVVIAYLVAVLVFGGHIGRGVKTSR
                     GFFIAEGRLGSLLVGLSLLGTYLSAMTMMALSGISFKDRYFKPDLDQEWNWLYTIQLP
                     FLVVTAIVITRWILPRYREAGVISVYELLERRIHVACRILASSCFLLLTVGRLGITIY
                     LPSLALAKVTGFDLTATILTMGLVVIIYTTLGGTEAVIWTDAVQVFVLVAGALFSLGY
                     ALFAHSGSPGGGFLQIAAEHGKFQMLDLELSLRKPVTLWFILETLVQTIRIFGTQQDM
                     TQRYMATSSTAKANRSVWIAILGYIPLAYLFYFMGTALFVFYFRNPDPNVFALAREGK
                     WDAVYPYFAVSRLPMGVAGLVIAAIFAAAQSTVSGSLNSAATVCVEDFYKRFGKRRRS
                     DPHYLTVARWLTVAWGVLATATAVLLKDIKEAQPVWNQIMAYSTNGVLALMVLALMRI
                     RVRPWAAITGFIAGGLCLFWVSYFKVVNFLLFAVIGNVVAFVVGLGLNALAGWFESGR
                     PGSDATEGA"
     gene            2358..>2718
                     /locus_tag="PLQ54_10505"
     CDS             2358..>2718
                     /locus_tag="PLQ54_10505"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 family protein"
                     /protein_id="HOS93735.1"
                     /translation="MEPAGHIISVSRRTDVPAHYAEWFLARVRQGFCEYVHPFSRKRF
                     RVSLRPADVLGIVFWSRDYRPMLPHLAALLEQYVFYCHLTLTGHGPALEPNAMPLETA
                     IAAARDVARLIGPEKLVW"
BASE COUNT          527 a          915 c          828 g          448 t
ORIGIN      
        1 gacactcgtt gcgcgtcagc agtcgtatgg cgttgccgcg ggcatccaca ctcgcgatgt
       61 cacccgacca cgtgctcccg gccttgagcc ccttgttggg gaagtccacc agcgtcgaca
      121 ggtggatcag tatggtccgt aagtcgagct cgtctcgctt cgcggcgagt ctcgggtcga
      181 cgaccacctc acggatgcag tggtcccgcc ccactttgac gaccagggtc tcaccgacgc
      241 agtctagccc cgtgcgactc cccaggacct cgtagaagcc cgagcgaaca tgcaggccca
      301 atgtggccgt ctccgcgtcg atgcccagca ccgccacacg gaaatctgcc tcggctctca
      361 gagcgaagtc gttcagttcg ttgtagacca acgagccggc caacgacagg tggaaatcct
      421 ggacgtggcc aacgatgggc cggtagacga gtcgtacgcg ctgggcggtg cgctccacct
      481 cggggaggtc cgactgcgcc actgaagcgc gtcccggcca cagtgtacaa aggagcgcgc
      541 cgaacgccag ccatcgggcc gccataccac cacccccacc ccagtcatgc cacagcctgt
      601 catccttccc caacccagac cagtggaatg tagttcctat gcgccctcgg tcgcatcgct
      661 ccccggcctg ccggactcga accaccccgc cagcgcattg agcccgagcc cgacgacgaa
      721 cgcgaccacg tttccgatca ccgcaaacag caggaagttg accaccttga agtagctcac
      781 ccagaacagg cacagccccc ccgcgatgaa cccggtgatg gcagcccacg gtcgcactcg
      841 gatcctcatc agagccagga ccatcaaggc cagaacgccg ttagttgagt aggccatgat
      901 ctgattccac acgggctgag cttctttgat gtccttgagc agaaccgctg tggccgtggc
      961 caggacgccc caggccacgg tgagccagcg ggccacggtt aagtagtgcg ggtcgctcct
     1021 gcgccgcttg ccgaaccgct tgtagaagtc ctctacgcac accgtggctg ccgagttgag
     1081 gctaccggag acggtggact gggccgccgc gaatatggcg gcgatgacca ggcccgccac
     1141 acccatggga agccggctca ccgcgaagta cgggtagacc gcgtcccact taccctctcg
     1201 ggccagagcg aagacgttcg ggtccgggtt acggaagtag aagacgaaca gggccgtgcc
     1261 catgaagtag aacaggtagg ccagagggat gtacccgagg atggcgatcc atacgctccg
     1321 gttggccttg gccgtcgacg atgtcgccat ataccgctga gtcatgtcct gctgcgttcc
     1381 gaagatgcgg atcgtttgga ccagtgtttc gaggatgaac cacagcgtga cgggcttcct
     1441 caaactcagc tccaggtcga gcatctggaa cttgccatgc tcagcggcga tctgcaggaa
     1501 gccgcctcca ggagagcccg agtgggcgaa caacgcatag cccagactga acagggcgcc
     1561 agcgacgaga acgaacacct gaaccgcgtc ggtccagatc acggcctcgg tgccgcccag
     1621 ggtcgtgtag atgatgacca ccaaccccat ggtgaggatg gtggccgtca agtcgaaccc
     1681 cgtcaccttg gccagtgcca gagacggcag gtagatggtg atgcccagac gtcccacggt
     1741 gaggagcagg aagcacgacg acgccaggat ccggcacgcc acgtggattc ggcgctccag
     1801 cagttcatag acgctaatga cgcccgcctc gcgatagcgc ggcaggatcc accgggtgat
     1861 gacgatcgcc gtgacgacca ggaacgggag ctggatcgtg tagagccagt tccactcctg
     1921 gtccaagtcg ggcttgaagt agcgatcttt gaagctgatg cccgacagcg ccatcatggt
     1981 catggccgag agatacgtgc cgagcagcga gaggcccacc agcagcgacc ccagccgccc
     2041 ctcggcgatg aagaaccctc gcgacgtctt cacaccccga ccgatatggc ctccgaagac
     2101 gaggacggcc accaggtagg cgattaccac agcccagtcg gcggtctcga accttggagc
     2161 gacgaactgc ggaatctcag tggcgggcat ccgggtcctc ccgttcgcgc cctgggcgcg
     2221 tcgcggccgc tgttccgggg cagccgactt ggcccctccc cgttccagac acacaagcct
     2281 gcagaagcac tcgccgccac cgcccatcgt cggccatctg ccttgagcgc tgggcgggtg
     2341 ccatataagg tagcggcatg gaaccggccg gccatatcat ctcggtgtca cggcggacgg
     2401 acgtccctgc gcattacgcc gagtggtttc ttgcgcgggt gcggcagggc ttctgcgagt
     2461 acgtccatcc tttctcccga aagcgttttc gggttagcct gcgcccggcc gacgtccttg
     2521 gcatcgtttt ctggtcgcgc gactaccggc cgatgctgcc tcacctggcc gcactgctcg
     2581 agcagtacgt gttctactgc cacctgacct tgaccggtca cggtccggcc ctggagccca
     2641 acgccatgcc gctggaaacc gccatcgcag ccgctcgcga cgtggctcgg ctgatcgggc
     2701 ccgagaagct cgtttggc
//
LOCUS       DAOSPL010000547         2714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_37475_length_2714_cov_2.981196, whole genome shotgun sequence.
ACCESSION   DAOSPL010000547 DAOSPL010000000
VERSION     DAOSPL010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37475_length_2714_cov_2.981196"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..887
                     /locus_tag="PLQ54_10510"
                     /pseudo
     CDS             60..887
                     /locus_tag="PLQ54_10510"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
     gene            1024..1887
                     /locus_tag="PLQ54_10515"
     CDS             1024..1887
                     /locus_tag="PLQ54_10515"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HOS93736.1"
                     /translation="MPLLRSLRPRQWAKNTLVLTALIFAGSATDPTSVARALAAMILF
                     CAAASGLYLINDLSDVERDRLHPSKRTRPIASGALAASTARIAAVALLGPSLVLGFLL
                     SVPFGLTLAAYAVMTLAYSAGIKQVAIADVFVLASGFVLRVVAGALVLDLAVSDWLLI
                     CALQVSLFMALCKRRHELGLLGGEAIEHRAALAHYSPGLLDQMIAIITSAILVSYSLY
                     TLWPDTVAHVGSTRLKYSIPFVMLGVFRYLQLVYVKDEGGAPERVLTDRGIVLIAVCW
                     AASVAAVIYGH"
     gene            complement(1896..>2714)
                     /locus_tag="PLQ54_10520"
     CDS             complement(1896..>2714)
                     /locus_tag="PLQ54_10520"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOS93737.1"
                     /translation="ECHVDGVYIDQVAAAAPAPCYDSTHGHPVGGGHYWTVDGYWPLM
                     ERVRRALPEGKFLTTECNAEPYAHLFDSYLTWHWQQQDAIPLFPAVYGCQIGMFGRSY
                     GAGPDRALATRMKAAQQLVFGEQIGWIGPDVIDRPDEGPFVRQAVRIRRALHDYIVDG
                     EMAHPPVVVGDVPTVTADWQWSGKWIVTTDALLTGSWRARDGRLVAIFANVSDAPISV
                     TWRFDGAAQGLKGPRLVVARRTETDVSESLTDLRFDRPLTVEPRQVVAFELRDQ"
BASE COUNT          424 a          999 c          845 g          446 t
ORIGIN      
        1 ggcgccaggc gctggcgcaa cggccactga cggccgcttt cgtcgaaccc atcagcccgc
       61 tgaccttctg tcgggagagc atcgagatcc tgaccgagtt cgcccgggcc ggtctcccgg
      121 tcggtatcgg cccgatggcc atctgtggcg tcacggcgcc cgctacgctc gcagggacgg
      181 tggtgcagga aaacgcggag atcctggcgg gcgtcaccat ggcgcagctg gtcgctcccg
      241 gactgccggt cgtctactgg ggcatccccc acgccatgga cgcgcggacc gcggggatca
      301 gcttcggcgc acccgagcag ggcctcctgg ccgtcgccat ggcccaactc gggcggcgct
      361 acggtctgcc gaccggcctc aacgtggggc tcacggactc gcttcttccc gacggccagt
      421 ccggcctcga gaagggcgcc actctgctga tgggggcgct cgccggcgcg gacatcttcg
      481 gccacatggg gattgccggg gcagaccagg gggcctcgct ggtccagctc gcggcagaca
      541 acgagtcgct cgcctatgtc cggcggatcc tctgaggcat ctcgctgtct gacgatcacc
      601 tggccgccga ggtgatccgt cgggtcggcc ccggaggcag cttcctgacc gaggagcaca
      661 cgctcgcgca ctaccgggac gagttatgga cgccgcagat cctgtaccga ggggcctacg
      721 agcgctggca gcagagcggc gccaaggaca tggccacccg agctgtggag ctggccgagg
      781 aggccgctgc gtcgccgggc ccgccgccat tgcccgagca cgtgcggcaa cagctcgtcg
      841 cgatcatgga cgaggcaagg ctcgcacttg gagccaccgc cagctaggac cagacccacg
      901 gggccactcg cccgggcatc accttgctcg ccgaggccag ccagtcctat catacgctcc
      961 ggcgcacacc ccctgtgtgt tcctcacaca ccgcacgagg ggccgcgcca gaggagtccg
     1021 cccgtgccgc tcctgcgctc gctccgcccc aggcagtggg ccaagaatac gctcgtcctc
     1081 accgcgctga tcttcgcggg ttcggcgacg gaccccacgt cagttgcgcg cgcactggcc
     1141 gccatgatcc tcttctgcgc cgccgccagc ggcctctacc tcatcaacga cctgagcgac
     1201 gtcgagaggg accggctgca tccatccaaa cgcacgcggc ccattgcctc cggcgcgctg
     1261 gcggccagca cggcccgcat cgccgccgtg gcccttctag ggcccagcct cgtcctgggc
     1321 ttcctgctgt cggtcccctt cggcctcact ctggctgcct atgcggtgat gaccctggcg
     1381 tacagcgcgg gcatcaaaca ggtcgccatc gccgacgtgt tcgtcctggc tagcgggttc
     1441 gtgctccgtg tggtggccgg tgccctcgtg ctcgacctcg ccgtctccga ctggctcctc
     1501 atctgcgccc tccaggtctc cctgttcatg gcgctgtgca agcggcgcca cgagctcggc
     1561 ttgctggggg gcgaggccat cgagcaccgt gccgcgttgg cccactactc gccggggctg
     1621 ctcgaccaga tgatcgccat catcacctcg gcgatcctgg tctcatactc gctctacacg
     1681 ctctggcccg acaccgtggc tcacgtcggc tcaacccgcc tcaagtactc aatcccgttt
     1741 gtcatgctcg gtgtcttccg gtacctgcaa ctcgtctacg tcaaagacga gggcggggca
     1801 cccgaacgcg tgctcaccga tcgcggcatc gtcctcatcg ccgtctgctg ggccgcctcg
     1861 gtcgccgccg tcatctacgg tcactagccg gtccgctact ggtcacgcag ctcgaacgcc
     1921 accacctggc ggggctcgac ggtaagtggt ctgtcgaacc gcagatccgt caggctctcc
     1981 gacacatcgg tctcggtgcg acgagccaca acgagacgcg gccctttcag gccctgcgcc
     2041 gccccatcga aacgccacgt caccgatatc ggcgcgtccg agacattcgc gaagatggcc
     2101 accagccgcc catccctggc ccgccacgag cctgtcagaa gagcgtcggt ggtgacgatc
     2161 cacttcccgg accactgcca gtcggccgtg accgtgggta catcaccgac gaccactggc
     2221 ggatgggcca tctccccgtc gacgatgtag tcatgcagcg cacggcgaat gcgaaccgcc
     2281 tgccggacga aggggccttc gtccggccga tcgatcacgt caggcccgat ccaaccgatc
     2341 tgctcgccaa acacgagctg ctgcgctgcc ttcatccgcg ttgccagcgc ccgatcgggc
     2401 ccggcaccgt acgaccgccc gaacatcccg atctggcagc cgtacaccgc cgggaacaat
     2461 ggaattgcgt cttgctgctg ccagtgccac gtcaggtagg agtcgaacag gtgagcatag
     2521 ggctcagcat tacactctgt ggtcagaaac ttaccctcgg gcaatgcgcg gcgaacacgc
     2581 tccatcaggg gccagtaccc atccacggtc cagtagtggc ctccgccgac gggatggccg
     2641 tgcgtcgagt cgtagcacgg tgccggagcc gcggccgcga cctggtcgat gtagacacca
     2701 tcgacatggc actc
//
LOCUS       DAOSPL010000548         2711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_37537_length_2711_cov_3.338855, whole genome shotgun sequence.
ACCESSION   DAOSPL010000548 DAOSPL010000000
VERSION     DAOSPL010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37537_length_2711_cov_3.338855"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PLQ54_10525"
     CDS             complement(<1..881)
                     /locus_tag="PLQ54_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOS93738.1"
                     /translation="MKIGVLSVVLGDMPLPAALDYLKGLGAEAVEIGAGNYPGQAHCP
                     VDELLSDASKRAAWKGEFDGRGLEISALSCHGNPLHPDDAVAKAHHETHRKAVQLAAQ
                     LGVKTVINFSGCPGGPSGGKEPIWVTAPWPDDFLKTVTWQWDEKVIPYWTVEAKYAKD
                     NGVNIGFEMHPNFVVYNPETLLKLRAACGDNLGANFDPSHLFWQGIDPCQAVRALEGC
                     IWHVHAKDCKVDPYNAPVNGVLDTKNYTDEIHRAWIFRTVGYGHGLDFWNAFVSNLRL
                     VGYDHVLSIEHEDSLMSG"
     gene            complement(1109..1285)
                     /locus_tag="PLQ54_10530"
     CDS             complement(1109..1285)
                     /locus_tag="PLQ54_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93739.1"
                     /translation="MSDARRDPRPLVVRKDSFYGYSSTHHTCKPYRGDGITSGLQPKQ
                     DERKINWRKAVVLD"
     gene            1550..2029
                     /locus_tag="PLQ54_10535"
     CDS             1550..2029
                     /locus_tag="PLQ54_10535"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HOS93740.1"
                     /translation="MAEPVRSLLLVCTGNTCRSPMAEALLTQRLRQAGLDTVVQSAGL
                     GAAEGCAATPEATEVAREFGVDLAAHRSEPLDAAMVESADLILTMTQSQLDCLLIRFP
                     AAEGKSHTLLGYAYGGQGVATDIEDPLGRGTSAYRAAAEIMVRAFEVIVRRVLLGNP"
     gene            2036..>2711
                     /locus_tag="PLQ54_10540"
     CDS             2036..>2711
                     /locus_tag="PLQ54_10540"
                     /inference="COORDINATES: protein motif:HMM:NF039894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOS93741.1"
                     /translation="MGGIVAAVEDGSIAAELGLRAGDEVIAIDGHPLRDLIDYRFHSS
                     ESEIEMLVRRATGEVLFEIEKDWSEDLGLHFTCPVFDGVRECNNRCVFCFVDQVAPGM
                     RESVRIHDDDYRLSFLAGNFVTLTNLGEDDLRRIVEWRLSPLYVSVHATDPKARARLL
                     RSPHHEAGLRNLRHLADAGIDFHCQIVLCPGINDGSVLESTVRELAALHPRVLSVGVV
                     PVGLTAF"
BASE COUNT          433 a          875 c          870 g          533 t
ORIGIN      
        1 cccgacatca ggctgtcctc gtgttcgatg gacagcacgt gatcgtagcc aaccaggcga
       61 aggttcgaca cgaaggcatt ccagaagtcg agtccatggc cgtagcccac ggtgcggaag
      121 atccaagccc gatggatctc gtcggtgtag ttcttggtgt ccagcacgcc gttgacgggc
      181 gcgttgtacg ggtcgacctt gcagtctttg gcgtggacgt gccagatgca tccctcgagg
      241 gctcgaacag cctggcacgg atcgatcccc tgccagaata ggtgacttgg gtcgaaattg
      301 gcgcccaggt tgtcgccgca ggcggcccgc agcttcagca gcgtctccgg gttgtagacg
      361 acgaaattgg ggtgcatttc aaacccgatg ttgaccccat tgtctttggc gtacttggcc
      421 tccacagtcc aatatggaat gactttctca tcccactgcc acgtcaccgt cttcaggaag
      481 tcgtcgggcc aaggcgcggt cacccagatc ggttccttgc ctccgctggg cccaccgggg
      541 cagcccgaga agttgatcac cgtcttcacg ccgagctgtg ccgccagctg cacggccttg
      601 cggtgggtct catgatgcgc cttcgccacc gcgtcatccg ggtgcagtgg gttgccgtgg
      661 caggacagag cgctgatctc gagcccacgg ccgtcgaatt cacccttcca cgccgcgcgc
      721 ttgctggcgt cgctgaggag ctcatctacg gggcagtggg cctggcccgg gtagttgcct
      781 gccccgatct ccaccgcctc ggctccgagc cccttcaggt agtccagcgc cgccggcagc
      841 ggcatgtccc caaggaccac ggaaagcaca ccgatcttca tcctgcagac tcctctcggc
      901 ggagcggacc ccgcccgata gtggcatcac gccacccctg cgacgcatgg cacgagccac
      961 tgaccgacgc tcgctccctg cgtcgcggag gggttcggcc tgggtggcgg cccgacctgc
     1021 cgccgcgtta ccagaacgcc gcgtccccgg acacggggcg cggcgttctg aacggcatgg
     1081 caggcagggc tataggacgt ggtggactct agtcgagaac cacggctttc cgccagttga
     1141 tcttgcgttc gtcttgcttg ggctgcaggc ccgaggtgat cccatcaccg cgatacggct
     1201 tacaggtatg gtgagttgaa ctgtacccgt agaacgagtc tttccggacg acgagcggcc
     1261 tgggatcacg acgtgcatcg ctcacgcagc atacctcctt ggcgtagtac tcggagcgac
     1321 cctagcgagg tctgccctga aggtcttcac cgggagatca ctgaactgcc ctgaggcctg
     1381 tgcaaccgcg tcttgtcacc gcagcctgtc atgggtctta ttcgtgtggc cgccgccgat
     1441 tccttctcgt ggggggcggt gagcgaggcg ccgcgcggtt tgacagccga gcgaccgtgc
     1501 ccaataatgt gccgcccgga gtccccatcg agcggaagcg agtccatcca tggccgaacc
     1561 tgtccgttcc ctgctccttg tttgcacggg gaacacctgt cgtagtccta tggccgaagc
     1621 gctgctcacc cagcggctgc ggcaggcggg gcttgatacc gtggtgcagt cggccggtct
     1681 gggcgccgcc gaaggctgcg ccgccacacc tgaggccacc gaggtcgcgc gggagtttgg
     1741 tgtggatctc gccgcgcatc gttccgagcc gcttgatgcg gcgatggtcg agtccgccga
     1801 cctgattctg acgatgacac agagtcagct ggactgcctc ctcatccgct tccccgcggc
     1861 agaaggcaag tcgcacaccc tgcttggcta tgcgtacgga gggcaggggg tggcgaccga
     1921 catcgaggat ccgctgggcc gaggcacctc ggcctatcgg gctgcggccg agatcatggt
     1981 gcgggccttc gaggtcatcg tccggcgcgt gctcctgggg aacccgtgat ggcgcatggg
     2041 agggattgtt gcagcggttg aggacggcag tatcgccgcc gagctcgggc ttcgcgctgg
     2101 tgatgaggtt atcgccattg atggccatcc gctccgcgac ctcatcgact accgcttcca
     2161 ctcatcggag tccgagatcg agatgctcgt ccgccgggcc acgggcgagg tgctcttcga
     2221 gatcgagaag gactggtccg aggacctggg gctgcacttc acctgtcccg tgttcgatgg
     2281 cgtccgcgaa tgcaacaacc ggtgtgtttt ctgcttcgtt gaccaggtcg ccccgggcat
     2341 gcgcgagtcc gtccgcatcc acgacgacga ctatcgtctc tcgtttctcg cgggcaactt
     2401 cgtcactctg acgaacctcg gcgaagacga cctgcggcgc atcgtggagt ggcgccttag
     2461 ccccctctat gtctcggtcc acgctaccga tcccaaggcg cgagcgcggc tacttcggtc
     2521 cccccatcat gaggcaggtc tgaggaacct gaggcacctg gccgatgccg ggatcgactt
     2581 ccattgtcag atcgtgctgt gtccgggcat caacgacggt tcggtactcg agagcacggt
     2641 gcgggagctg gctgccctcc acccaagggt tctgtcggtg ggagtggttc cagtgggtct
     2701 gactgcgttc c
//
LOCUS       DAOSPL010000549         2711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_37542_length_2711_cov_2.896837, whole genome shotgun sequence.
ACCESSION   DAOSPL010000549 DAOSPL010000000
VERSION     DAOSPL010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37542_length_2711_cov_2.896837"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PLQ54_10545"
     CDS             <1..778
                     /locus_tag="PLQ54_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOS93742.1"
                     /translation="HQVIRSLFQATIRAAEILGCDAEFAARLARLSDRIAPNQIGRHG
                     QLQEWLEDWDDPNEQHRHVSHLWGLHPGAEITPRGTPELAAAARQSLLFRGDGGTGWS
                     KAWKINFWARLLDGEHAHKMLVEALAGNTLPNLFDTHPPFQIDGNFGATSGIAEMLLQ
                     SHAGEIDLLPALPAAWPSGFFHGLRARGDFTVDLEWADGALVRATVHSGTTGVCRVRV
                     PGTRGVRVDGAGSVRAPEPGVVEFEARAGEHYRLQAEPPA"
     gene            845..>2711
                     /locus_tag="PLQ54_10550"
     CDS             845..>2711
                     /locus_tag="PLQ54_10550"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOS93743.1"
                     /translation="MDHRPIRNLDAVPIQQVTLNDPFWSPKMDVWRRVTLIDCFNKLE
                     ANGALANFDLVARGEAGEHGGPPWYDGLLYETIRAAGDFLAAGRDATLEARVDGYVER
                     IAAAAARDPDGYVNTWTQLKQPGQRWGMNGGDDNWQHDLYNAGALVEAAIHYYRATGR
                     IRLLELATRLANHMVDVMGPPPKANVVPGHSIAEEAFVRLHLLFGEQPELRQRMPVPV
                     DAGAYLRLAQFWIENRGHTEGRRSFGPYGQDHVPVLEQETIEGHAVRATLLCAGLVEA
                     GVAAGRSDYLDAAVRLWENMVCRRMYITGGLGAVAGYEGFGPDYALPNTGYVETCAAV
                     GAGFFHHNLSLALGHGRYADELERVLYNAVLCGTSLVGDSYFYENPLEAGPDRRRWAW
                     HPCPCCPPMFLKMMAAMPGYLYATDGQQLVVNLLVGSEARVSLGGVPVRVCQTTEYPW
                     REDVELGINPEGPARFGVAVRIPGWCDGATFALNGEPVRAPQVADGYARFWREWRPGD
                     RLELQLPMPVRRMKAHPAVQDAAGRIALTRGPVIYCVEGIDCAVRPRHLIVPPDASLT
                     AEHRADLLGGVTAIRGLGLTLRRQADDAPLYRRADDRPETHPVEFTAIPYYANANR"
BASE COUNT          434 a          913 c          931 g          433 t
ORIGIN      
        1 ccatcaggtc atccgcagcc tgttccaggc caccattcga gccgccgaga tcctcggctg
       61 cgacgccgag ttcgcggcgc ggctcgccag gctcagcgac cgcatcgcac caaaccagat
      121 cggccggcac gggcaactcc aggagtggct cgaggactgg gacgacccga atgaacagca
      181 ccggcacgtg tcgcacttgt ggggcctgca cccgggcgcg gagatcacgc cgcgagggac
      241 gcccgagctt gcggcggccg ccaggcagtc cctgctcttc cggggcgatg gcggcacggg
      301 ctggagcaaa gcgtggaaga tcaacttctg ggctcggctg ctcgacggcg agcacgcgca
      361 caagatgctg gttgaggcgc tcgccggaaa cacactgccc aacctgtttg acacgcaccc
      421 cccgtttcag atcgatggca acttcggtgc cacatcaggt atcgccgaga tgctactgca
      481 gagccatgcg ggcgagatcg atctactgcc ggccctacca gccgcatggc cgtccgggtt
      541 tttccatggc ctgcgggctc gaggcgactt cacggtggac ctcgagtggg ccgacggcgc
      601 gctggtgcga gccacggtcc attccggcac aaccggcgtg tgccgcgtcc gcgttcccgg
      661 gacccgcggg gttcgggtcg acggagcggg cagcgttcgc gcaccggagc ctggggttgt
      721 ggagttcgag gcccgcgcgg gtgagcacta ccgcctccag gcagagccgc ccgcgtagcc
      781 gaccgccaca ccgaagccgt tggcgccgtc ggtgcacggc cgccatggaa ggagctcacg
      841 gacgttggat caccgcccga tccgcaacct cgacgcagtg ccgatccagc aggtaacgct
      901 gaatgaccca ttctggtcgc cgaagatgga tgtctggcgc cgagtgactc tcatcgactg
      961 cttcaacaag ctcgaggcga atggtgcgct ggcgaacttc gacctcgtcg ctcggggcga
     1021 ggccggtgag cacggcggcc cgccgtggta tgacgggctc ctttacgaga ccattcgggc
     1081 cgcgggggac ttcctggccg ccggacgcga tgcaacgctc gaggcacggg tcgatggcta
     1141 cgtggaacgc atcgcggcgg cggccgcgcg agacccggac ggctacgtca acacgtggac
     1201 gcagctcaag cagccgggcc agcgctgggg catgaacggc ggcgatgaca actggcagca
     1261 tgatctgtac aacgccggcg cgctcgttga ggcggccatc cactactacc gggccaccgg
     1321 caggatccgg ctgcttgagt tggccactcg cttggccaac cacatggtcg atgtgatggg
     1381 gccgccgccg aaagccaacg tggtcccggg ccactcaatc gccgaggagg cattcgttcg
     1441 cctccacctc ctgttcggcg agcagcccga gctccggcag cgcatgcctg tgcccgtcga
     1501 tgcgggcgct tacctgcggc tcgcccagtt ctggatcgag aaccgcggcc acaccgaagg
     1561 gcgacgatcg ttcggcccat atggccagga ccacgtcccg gtgctcgaac aggagaccat
     1621 cgaggggcac gccgtacgcg ccacactgct atgcgcgggg ctggttgagg cgggagtagc
     1681 ggcgggacgc tccgactacc tcgacgcggc ggtccggctc tgggagaaca tggtctgccg
     1741 ccggatgtac atcaccggtg gcctgggtgc cgtggccggt tatgagggtt tcggaccgga
     1801 ctacgcgttg ccgaacacgg gctacgtcga gacctgcgcg gccgtgggcg ctggcttctt
     1861 ccaccacaac ctgagcttgg ccctgggcca cgggcgctat gccgacgagc tggagcgcgt
     1921 gctctacaac gcggtgctct gcgggacctc tctggttggt gactcgtact tctacgagaa
     1981 cccgctcgag gccggccctg atcgtcgccg ctgggcgtgg cacccctgcc cctgctgccc
     2041 tcccatgttc ctcaagatga tggccgcgat gccggggtat ctctacgcta ccgacggcca
     2101 gcagcttgtg gtgaacctgc tcgtgggcag cgaggcccgt gtctcgctgg gcggtgtccc
     2161 ggttcgggtg tgccagacca cggagtaccc gtggcgggag gacgtcgaac tgggcatcaa
     2221 cccggagggc ccggcacgat tcggtgtggc tgtccgcatc ccgggttggt gcgatggcgc
     2281 gacgtttgcg ctcaacggcg agccggtgag ggcgccccag gtcgcggacg gatatgcccg
     2341 cttctggcgc gagtggcgtc ccggcgacag gctggaacta cagctcccga tgcccgttcg
     2401 ccggatgaaa gctcacccgg ccgtgcagga tgccgccggg cgaatcgcgc tgacgcgcgg
     2461 tccggtcatc tactgcgtgg agggcatcga ttgcgccgtg agaccacggc acctgatcgt
     2521 tccgcccgat gcctctctga cggctgagca tcgggccgat ctgctcgggg gagtgacggc
     2581 catcagaggc ttggggctga cactccgccg gcaggccgac gatgctcccc tgtaccgccg
     2641 agctgacgac cgccccgaga ctcatcccgt cgagttcacc gcgatcccgt actacgccaa
     2701 cgcaaaccgt g
//
LOCUS       DAOSPL010000550         2706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_37639_length_2706_cov_2.697850, whole genome shotgun sequence.
ACCESSION   DAOSPL010000550 DAOSPL010000000
VERSION     DAOSPL010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37639_length_2706_cov_2.697850"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..>2706)
                     /locus_tag="PLQ54_10555"
     CDS             complement(156..>2706)
                     /locus_tag="PLQ54_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93744.1"
                     /translation="WLWVCCLAWAALLAGTAVAEDERDQKDLVRYADRAMRGFMVTWP
                     EPIPDPPGALRTCFDCMHLPVPRLSHAQWDWGDCTARAARDWLYVREMTGDRDFGRDV
                     EEGQRQALLSCLAPETGMVFVPERSRPDQGVYHYQAWDQGRTLRALVWWWLAETDSAR
                     KAEMAARIDRMVAGLSALAVRGHDPVYGPYAVYPFDYTEGDRHGDDLRCMRAGQLVEP
                     LAVYWENGGGAQAREFADEIVAGILSGHEGDTYAPPIDGWFRFGEDGAFHHHFHAHTS
                     TVLGVARMGQALVRKGERAKGLPLLEWAKRVYDWTLSPANANAGSTWGWFPENMGSDN
                     TQAREISEICCTADMIELASVLAESAALDARLASWDALWDHVERYTLNTIVPAQFSVT
                     PRYRRLLERSLRDRVAAHNGYIELELDRLGAYNHEIVGHQTLRVAGIGHLSQAVYAVE
                     YDGQRAWCSYVPGQPTASRGLAVESATHREGDALAGSVRTEDGRLRIDHGAACGRGPY
                     VLRTFSVTNTSAEPLAGARLACATNLDSPTWSAEQGTIADGCARIAARGEEPCVGLKA
                     EPRPAFMSLSEAGPSLAGEKALDWSAPRDRYFGNSAVLVGWELGTLSPGETRAVTMVM
                     AVGSSGEELDRALRQETFPPLEGGSALEIELRRAERLNGSWVATFFPNDLAALGPDST
                     LTLSAMGCCPYAGVRALYSCWKPALADDAQTISARYAIDRVSAVAVQEAMQDARRVTQ
                     SISLLAGRALRVRLPDWAHPSSVLATGGGGRRVPFHVRGQWLEFGRLAAGSEVTVSYP
                     VRLRVTRERVGGAGTSQGFAPADQKRTFTAYWRGNIVVRVEPRGELMPMFE"
BASE COUNT          417 a          984 c          896 g          409 t
ORIGIN      
        1 gacgacgggc ggcatgggac cgcaccgaag gctacggacg cggcgctcac cccatcggcc
       61 caccgatcac ccgatagcga agacagttcc tggttgccca gccaagtggg ggcggtgcca
      121 gcaggtgccg cccccgcttc gtttcggccg atgtcttact cgaacatcgg catgagctca
      181 cctcgcggct caacgcggac cacgatgttg ccacgccagt atgcggtgaa ggtgcgcttc
      241 tggtcagccg gcgcgaaccc ctgggatgta cctgcccctc cgacgcgctc ccgggtcacc
      301 cgcagccgaa cgggatacga caccgtgact tcgctgccag cggccagacg gccgaactcg
      361 agccactgtc ctcgcacgtg gaacggcacg cgccgtcccc ctccgcctgt cgcgagcacg
      421 gaactggggt gcgcccagtc cggtagccgc acgcggagcg ctcggcctgc cagcagggag
      481 atgctctggg tcaccctccg cgcgtcttgc atggcctcct gtaccgcgac agccgagacc
      541 cggtcgatgg cgtagcgggc cgagatcgtc tgggcgtcgt ccgccagcgc cggcttccag
      601 cacgagtaga gcgcacggac cccggcgtac gggcaacacc ccatggcgct gagtgtgagg
      661 gtactgtccg ggcccaacgc cgccagatcg ttcgggaaga aggtggctac ccacgagccg
      721 ttgagccgct cggctcggcg caactcgatc tccagcgccg atccgccctc gagcggcggg
      781 aaggtctcct gccgcaacgc ccggtcgagc tcctcgccgc tcgagccgac agccatcacc
      841 atggtcacgg ctcgtgtctc acccgggctc agcgtcccca actcccagcc caccagcacg
      901 gctgagttgc cgaagtagcg gtcgcgcggt gcggaccagt cgagcgcctt ctcgcccgcg
      961 aggctgggcc ccgcctcgct caggctcatg aacgccggac gcggctctgc cttgagtccg
     1021 acgcacggct cctcgccccg agcggcgatg cgggcgcatc catcggcgat cgtcccttgc
     1081 tccgcgctcc acgtgggcga atccaggttg gtcgcgcatg ccagacgggc gccggccagc
     1141 ggctctgcgg acgtgttcgt gacggagaag gtccgcagga cgtagggccc gcggccgcat
     1201 gcggcgccgt ggtctatgcg caggcggccg tcttcggtcc gtaccgatcc ggccaacgcg
     1261 tcgccctcgc ggtgggtggc cgactcgacg gccagcccgc gcgacgctgt tggctgaccg
     1321 gggacatacg aacaccaagc ccgctggcca tcgtattcga cggcatacac tgcctgcgac
     1381 aggtgcccga tcccagccac tcggagcgtc tgatgtccca caatctcatg gttgtaggcg
     1441 cccaaccgat ccagctcgag ctcgatgtag ccgttgtgcg cggccacccg gtcccggaga
     1501 ctgcgctcaa gcagtcgccg gtaacggggc gttaccgaaa actgcgcggg gacgatggtg
     1561 ttgagggtgt agcgctcgac atgatcccac agcgcgtccc agctcgctag tctggcgtcg
     1621 agggcagccg actccgcgag caccgaggcc agctcgatca tgtcggccgt gcagcagatc
     1681 tcactgatct cgcgcgcctg ggtgttatcc gaccccatgt tctcggggaa ccagccccag
     1741 gtcgacccgg cgttggcatt tgccggagac agcgtccagt cgtagacgcg cttggcccac
     1801 tcgagcagcg ggagcccttt ggctcgctcg cccttccgaa cgagtgcctg gcccatccga
     1861 gccacaccga gcactgtcga tgtgtgggcg tggaagtggt ggtggaacgc cccgtcctcg
     1921 ccgaagcgga accacccgtc gatgggcggc gcgtacgtgt cgccctcatg ccccgagagg
     1981 atgccggcca cgatctcgtc ggcgaactcg cgggcctggg ctccgccacc gttctcccag
     2041 tagacggcga gtggctcgac gagctggccc gcgcgcatgc accgcaggtc atcgccgtgg
     2101 cggtcgccct cggtgtagtc gaaggggtac acggcgtagg ggccatagac cggatcgtgg
     2161 ccgcggaccg ccagggcgct caggccggcg accatccggt cgatgcgcgc ggccatctcc
     2221 gccttccgcg cggagtccgt ctcggccagc caccaccaga ccaacgcacg cagcgtgcgg
     2281 ccttggtccc acgcctggta gtggtacacg ccctggtccg gccgggatcg ttcgggcaca
     2341 aagaccatgc cagtctccgg tgcgaggcac gacagcaacg cctgccgctg cccctcctcg
     2401 acgtctcggc cgaagtcgcg gtcgcctgtc atctcgcgca cgtagagcca gtcgcgcgcg
     2461 gcgcgggcgg tgcagtcgcc ccagtcccac tgggcatggc tgagccgcgg tacgggcagg
     2521 tgcatgcagt cgaagcaggt ccgcagtgcc ccgggtgggt ccgggatggg ctccggccag
     2581 gtcaccatga acccgcgcat tgcccgatcg gcgtagcgga ccaggtcctt ctggtcgcgc
     2641 tcatcctccg cgacggccgt gccggccaac agcgccgccc aagccagaca gcagacccac
     2701 agccat
//
LOCUS       DAOSPL010000551         2703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_37690_length_2703_cov_3.527190, whole genome shotgun sequence.
ACCESSION   DAOSPL010000551 DAOSPL010000000
VERSION     DAOSPL010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37690_length_2703_cov_3.527190"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="PLQ54_10560"
     CDS             <1..1257
                     /locus_tag="PLQ54_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOS93745.1"
                     /translation="RSMHSPLVPSGGKLPPQWRVYAYGGLVLAALFLLYRLRSILPPF
                     LAGVIVAFILDPFLDRLQRRGWSRTKAVGFVYAVSLTTLILLALLVIPPIVGYVGSWV
                     ERIQGIATDLAQVLHVESLEEFEARLEARFPALADHIDVDWVALAPDIQRAREGLLRT
                     VESKSPQWAQQAAGILYRVIAGSASTLMSIIFVPLTAFYFSIEIDPLRARVMNWVPED
                     YRESVLRVGADVNEMLSSFFRGQLALMGMVGTSALVTMLVIGRVWHIELGAVLFLSLW
                     YGLTYCIPIVGAVSSLALAAGTCFVLSGYNWVCAAVALGVFWAINLVFDNVVTPRVVG
                     HKVGLHPLVVLFGVFAGYHVLGLLGMIVAVPLAALVKIVIDEFLPEVFARLPGQGDPA
                     MEDGASVPAASDDGREPPPPAPTEED"
     gene            1265..>2703
                     /gene="alaS"
                     /locus_tag="PLQ54_10565"
     CDS             1265..>2703
                     /gene="alaS"
                     /locus_tag="PLQ54_10565"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOS93746.1"
                     /translation="MATANSGWSTDRIRQTFLDYFASKDHRVIPSVSLIPTSPSTLLT
                     GAGMQPLVPMFRGEEEPPAPRLASSQKCVRTGDIENVGRTARHHTFFEMLGNFAFGDY
                     FKREAIEFCWELVTQGFELPPDALWATVYEEDDESWQLWHRHIGLPAHKVIRCGKKDN
                     WWPQTVWNGPCGPCTEVYVDQGPGIYPCDNAQCGPECDCDRYLEIWNLVFQMYNQTEE
                     GALSPLPTPGVDTGLGLERLAAVKQGVPTNFDIDIFRPMIAAIEAEAAATGHPFHYGD
                     GPRTDQAVRVIVDHFRAIAFMMADTIMPSNKGRGYVLRRIVRRALRFARHLGLDDPFM
                     FRLLPALIDTMGHVYPELTQRSNTIVASVRQEEERFCATLEQGIGILSGLVADAKAAG
                     RSALPGEEVFRLYDTFGFPVEMTEEMAAEEGLTIEREGFEAALERQRSRSVFRFTESA
                     AAKEFYVSLSRAHPASEYARDQAALADLRV"
BASE COUNT          406 a          954 c          855 g          488 t
ORIGIN      
        1 cgatcgatgc atagccccct ggtcccttcc gggggcaaac tgccccccca gtggcgcgtc
       61 tacgcgtatg gcgggctggt tctggctgcc ctctttctcc tctatcggct ccggtcgata
      121 ctgccgccct tcctggccgg cgtcatcgtc gcgttcatcc tcgatccgtt cctcgaccgc
      181 ctccagcgcc gcgggtggtc gcgaaccaag gcggtgggct tcgtctacgc ggtctcgctg
      241 actacgctca tcctcctggc cctgctcgtc atcccgccca tcgtggggta cgtgggatca
      301 tgggtcgagc gcatccaggg catcgccacc gacctcgccc aagtgctcca cgtggagagc
      361 ctggaggagt tcgaagcccg ccttgaggcc cggttcccgg ccctggccga tcatatcgac
      421 gtcgactggg tcgccctggc gcccgacatc cagcgtgccc gcgaaggcct cttgaggacg
      481 gtcgagagca agtcccccca atgggctcaa caggccgcgg gcatcctcta tcgcgtcatt
      541 gccggctcgg cctccacgct gatgtccatc atcttcgtcc ccttgacggc cttctacttc
      601 tcgatcgaga tcgacccgtt gcgggcccgg gttatgaact gggtgcccga ggactaccgg
      661 gagagcgttc tgcgcgtggg ggccgacgtc aacgagatgc tgtcctcctt cttccgcggg
      721 cagctcgcgc tcatgggcat ggtcggcaca tcggccctgg tgaccatgct ggtcatcggt
      781 cgggtatggc acatcgagct gggtgccgtg ctgttcctgt cactgtggta tggtctcacc
      841 tactgcatcc ccattgtggg cgccgtgagt tcgctggcgc ttgccgcggg cacgtgtttc
      901 gtcctctccg gctataattg ggtctgcgcg gcggtggccc tcggcgtctt ctgggccatc
      961 aacctggtgt tcgacaatgt cgtcaccccg agagtcgtcg gccacaaggt cggcctacac
     1021 ccgctggttg tcctgttcgg ggtcttcgca ggctaccacg tactggggct cctgggcatg
     1081 atcgtggccg tcccgctggc cgccttggtg aagatcgtca tcgacgagtt cctgccggag
     1141 gtcttcgcga ggctgcccgg acaaggcgac ccagccatgg aggacggcgc gtccgtgccg
     1201 gcggcgtcgg acgacggtcg cgaacccccg ccgcccgccc ctactgaaga ggactgaggc
     1261 aagcatggcc acggcgaact cgggctggtc cacagaccgg atccggcaga cattcctcga
     1321 ctacttcgcg tccaaggacc accgcgtcat ccccagcgtg tcgctgatcc cgaccagccc
     1381 ctccacgttg ctgaccgggg ccgggatgca gccgctggtg cccatgttcc ggggcgagga
     1441 ggaaccgccg gcgccccgcc tcgccagctc gcagaagtgc gtgcgcaccg gcgacatcga
     1501 gaacgtgggc cgcacggccc ggcaccacac gttcttcgag atgctgggca acttcgcgtt
     1561 cggcgactac ttcaagcgcg aggccatcga gttctgctgg gaactcgtca cccagggttt
     1621 cgagcttcct cccgatgcgc tctgggccac cgtctatgag gaagatgacg agagctggca
     1681 gctctggcac cggcacatcg gcctccccgc ccacaaggtc atccgctgcg gcaagaagga
     1741 caactggtgg ccgcagaccg tgtggaacgg accgtgtggg ccctgcaccg aggtctacgt
     1801 cgaccagggc cccggcatct acccctgcga caacgcccaa tgcgggcccg agtgcgattg
     1861 cgaccgctac ctggagatct ggaacctggt attccagatg tacaaccaga ccgaggaagg
     1921 cgccctctcg ccgctgccaa cgcccggtgt cgacacgggc ctgggcctcg agcgcctcgc
     1981 ggccgtcaag cagggcgtcc ccaccaactt cgacatcgac atcttccgcc ccatgattgc
     2041 ggccatcgaa gccgaggccg ccgccaccgg gcatccgttc cactacggcg acggtccgcg
     2101 cacagatcag gccgtgcgcg tcatcgtgga ccacttccgc gccatcgcgt tcatgatggc
     2161 cgacacgatc atgccctcga acaagggccg cgggtacgtg ctccgtcgca tcgtgcggcg
     2221 agccctccgc ttcgcgcggc acctggggct ggacgacccg ttcatgttcc gcctgctccc
     2281 cgcgctcatc gacacgatgg gacacgtgta tcccgaactg actcagcgga gcaacaccat
     2341 cgtggcctcg gtccgccagg aggaggagcg cttctgcgcc acactggagc aggggattgg
     2401 catcctgtcc gggctcgtgg ctgacgccaa ggccgccggc cggtcggccc tgcctggcga
     2461 ggaggtcttc cgcctctatg acaccttcgg cttcccggtg gagatgaccg aggagatggc
     2521 cgccgaggaa gggctgacca tcgagcgaga ggggttcgag gccgccctcg agcgccagcg
     2581 gagccgcagc gtctttcggt tcaccgagtc cgccgcggcc aaggagttct acgtgagcct
     2641 cagccgcgct cacccggcat cggagtatgc tcgcgaccag gccgccctgg cggacctccg
     2701 cgt
//
LOCUS       DAOSPL010000552         2703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_37695_length_2703_cov_3.010196, whole genome shotgun sequence.
ACCESSION   DAOSPL010000552 DAOSPL010000000
VERSION     DAOSPL010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37695_length_2703_cov_3.010196"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="PLQ54_10570"
     CDS             <1..1034
                     /locus_tag="PLQ54_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93747.1"
                     /translation="AENGARPAHQVLAGPGGEFSLPGVVGSQPRVLAWTPDGQVANRM
                     LDTRGGQFPGQVTDPPPLELSLAPMPAITGWVVTPPGDPATSCIDVVAEPTQIWDTSG
                     PWPAGTMCARVHAGPDGAFAIEGLKPGEYSVTAVWGTLRRTAPVVVDTFQGGVGGLVL
                     TAEPLVPTLRLRLENDRSRPLAPGQPLGVSVNGSRLAGPMALVDGWVELCHTPPGLYQ
                     VWLVALAHDGTLTGSGDVTPLTSELSVRPSQWPRLRGRVVAAPGHGLHSGLRVALCSA
                     DQWMGSPTYYWDIEPDGTYDVPTRVAGELEVSLVAGRATRLAMHRVTVTDQAVTEVPD
                     LVYAGLPSD"
     gene            1077..>2703
                     /locus_tag="PLQ54_10575"
     CDS             1077..>2703
                     /locus_tag="PLQ54_10575"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOS93748.1"
                     /translation="MRWCLLSTLVVLVVSGGCHRRAEGKPAGGSAAEPALYYAIRIGD
                     AHAGYAEIRGPSLVRNADGTARVYSSHTVLRIAMLGQPRPMSWKSEVRIATDSDRLTG
                     YEATIRQGETETRIACRPEGSILRLERQVTGDAEADTAQIEVPENHYFMVGNDFALWM
                     LLARRFAPSPGNLVRVQAVSGEAMVAQSVELKGLEPKPITRGGQQVQARRVAVGGEAI
                     LWADAGTGELLRIEIPAQNAVIERADAAIIAEAERAEPVDPLASRFTPSEVKFADIKA
                     VTMLRAEIDVDVTTGAGATVCTDSPMQRFGGRVEGTTIRGTVRVRSTAYDGKDAAPLP
                     MVPGKLPATVRRYLDPEPMIESDDAGIRTQAVEVAGRATTAWEAACAITDWVESHVAK
                     DRIADTPSAKRALATRSGDCGPHATLATAMLRALGVPAKLVGGLMYSPSLAGSFGQHA
                     WVEVFVGGDRWVPVDPMTGETRSLSALHIKCFEGMGGAIPRRVRVLEYEPRTEVTRAE
                     ASASVPRLTWELNHTYTWVYMQGERTMGSEAVCF"
BASE COUNT          417 a          882 c          985 g          419 t
ORIGIN      
        1 gggctgagaa cggagccagg ccagcgcacc aggttctcgc cgggcccggc ggcgagttca
       61 gcctgcctgg cgttgtgggg agccagccgc gcgttctcgc ttggacgccc gacggccagg
      121 tggcgaaccg gatgctggac acgcgcgggg ggcagttccc agggcaggtc accgacccac
      181 cgcccctcga actctcgctt gcacccatgc cggccattac gggctgggtc gttactcctc
      241 ccggcgaccc ggcgacgagt tgcatcgacg tggtagctga acccacccag atctgggaca
      301 ccagtgggcc atggccggcc ggtacgatgt gcgcccgcgt acacgcgggc ccggacggtg
      361 cgttcgccat cgaggggctc aagccaggtg agtacagcgt tacggcggtg tggggtacgc
      421 tccggcggac cgcacccgtg gttgtggaca ccttccaggg cggcgtcggt ggactcgtgc
      481 tgacagccga gcccttggtt cccacgttga ggctcagact cgagaatgac aggagccggc
      541 ccttggcacc ggggcaacca ctgggtgtct cggtcaacgg gtcgcgcctg gcgggaccca
      601 tggctctggt ggatggctgg gtcgagctgt gccacacgcc cccgggcctc tatcaagtgt
      661 ggctggtggc gctggcccat gacggcacgc tgactgggtc gggagacgtc acgccgctca
      721 catcggaact gagtgtgcgc ccgtcgcagt ggccacgcct gcgcgggagg gttgtcgccg
      781 cgccgggcca tgggctgcat tctggactgc gggtggcact ctgctccgcg gatcagtgga
      841 tgggatcgcc gacatactac tgggacatcg agcccgacgg cacttacgac gtccccaccc
      901 gtgtcgcggg cgagctcgag gtaagtctcg tggccgggcg cgcgactcgg ctggccatgc
      961 acagggtgac cgtgacggac caggccgtga ccgaggtgcc cgacttggtg tacgctgggc
     1021 ttccgtctga ctagcgccgc gacagggcgc tggcggcgga tggagggccg cagttggtgc
     1081 gatggtgctt gctgtccacg ctggtggtct tggtggtgtc gggtgggtgt caccggcgcg
     1141 ccgaggggaa gcccgcgggc ggctcagccg ccgagcccgc actctactat gcgatccgca
     1201 tcggtgatgc gcacgcgggc tacgccgaga tccgaggccc atcgctcgtc cggaatgctg
     1261 acgggaccgc tcgtgtgtac tcctcccata cggttctccg catcgccatg ttgggccagc
     1321 cccgccccat gagttggaag tccgaggtgc ggatcgccac ggattccgac cggctgaccg
     1381 ggtacgaggc caccattcga caaggggaga ccgagacacg catcgcgtgc cgccccgagg
     1441 ggagcatcct caggctcgaa cgccaggtca ccggagacgc tgaggcggac acggcccaga
     1501 tcgaggtgcc ggagaaccac tacttcatgg tcgggaacga ctttgccctc tggatgctcc
     1561 tggcccgccg gttcgccccc agcccgggga acctggtccg tgtgcaggcg gtatcgggcg
     1621 aggccatggt cgcccagagc gtcgagctca agggactgga gccgaagccg atcacgcgcg
     1681 gcggccaaca ggtccaggcg cggcgcgtgg ccgtgggagg cgaggccatc ctctgggccg
     1741 acgccgggac cggtgagttg ctgcggatcg agatccccgc acagaacgct gtgatcgagc
     1801 gcgccgacgc ggcgatcatc gccgaggccg agagagccga gccggtggat ccgctggcga
     1861 gccgcttcac gccgtccgag gtgaagttcg ccgacatcaa agccgtgacg atgctccggg
     1921 ccgagatcga tgtcgatgtg acgaccgggg ccggggccac ggtgtgcacc gacagtccca
     1981 tgcagcggtt cggcggccgg gtcgagggga ccaccatccg tggcacagtc cgtgtccgat
     2041 cgaccgcgta cgatggcaaa gacgcggcgc cgctgccgat ggtccccggc aagctgccgg
     2101 ccacggtgcg acggtacctg gatcccgagc cgatgatcga gtcggacgac gcaggtatcc
     2161 gcacccaagc cgtcgaggtc gccgggcggg ccaccactgc ctgggaggcg gcatgcgcca
     2221 tcaccgattg ggtcgagagc catgtggcca aggaccgcat cgcggacaca ccctccgcca
     2281 agcgggcgct ggcgacccga tcgggcgact gtgggccaca cgcgacgctg gccacggcca
     2341 tgctgcgcgc cctcggcgtg ccggccaagc tggtcggcgg gctaatgtac tcgccatcgc
     2401 tggcgggtag cttcgggcag catgcctggg tcgaggtgtt cgtcggcggc gatcgctggg
     2461 tgcccgtgga cccgatgacc ggcgagactc gttcgctttc ggcgctgcac atcaagtgct
     2521 tcgaaggcat ggggggcgcc atccccaggc gcgtgcgggt gctggagtac gaaccgcgca
     2581 ctgaggtgac gcgtgcggag gcatcggctt ctgtcccgcg tctgacctgg gagctcaacc
     2641 acacgtacac atgggtgtac atgcagggag aacgcaccat gggatccgag gccgtgtgct
     2701 tct
//
LOCUS       DAOSPL010000553         2700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_37756_length_2700_cov_2.844612, whole genome shotgun sequence.
ACCESSION   DAOSPL010000553 DAOSPL010000000
VERSION     DAOSPL010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37756_length_2700_cov_2.844612"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PLQ54_10580"
     CDS             <1..686
                     /locus_tag="PLQ54_10580"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOS93749.1"
                     /translation="TWGWAFGGQFYDDVNRRITCTDPKIVRALEWMTSYADRYDVTKI
                     ASLQAGFGTAEQNPFYTGRLAMTCLLIAGIADIERFAPELDYGVAPQPMPDGGEKDSS
                     WVGGWCIALPRGARHQDEGFEFIRWLCHDPGGTQTVGRETGLFPGMRHSPYFDEVRNR
                     KYYSTFLRILEACKHQRPVMPVQAFYMRQMQRAVDSAIYKQKTAKQALEDAEKATQAE
                     LDLVLRGGE"
     gene            complement(753..1289)
                     /locus_tag="PLQ54_10585"
     CDS             complement(753..1289)
                     /locus_tag="PLQ54_10585"
                     /inference="COORDINATES: protein motif:HMM:NF025049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOS93750.1"
                     /translation="MSVNTEKAISALEYALKTEVDGRKYYETIAANTKSPLVASTFRA
                     LAQDEIGHIHAINDYIATLKQAGSWKDFSLTARVSDLSGEVMTMFREAMEGETATAAA
                     PDDTEAYRQAAGFEKKGIEFYGEQASAATDDQARAFWSFLADEERRHLALIEETMGYL
                     DHPEDWFLIGERGLLDGG"
     gene            complement(1384..2421)
                     /locus_tag="PLQ54_10590"
     CDS             complement(1384..2421)
                     /locus_tag="PLQ54_10590"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS93751.1"
                     /translation="MRTEYVAVPVAQAIREDGVAAAIQKKHVASVLYTYRCTLACRHC
                     LFSCSPRKPNVHVGLEDGLEYLRRLHQTDRVVHIAGGEAMMYWSALIDLCREAGREGV
                     APHFVETNATWCISDALAYRRLEQLRDAGVLGLLISACPHHQHECPAENYDRCFRAAV
                     EVFGDRNVAAARLTMDELRDFEAIARDEERLADYVRANPPCLVGRAGEALARYLPDRP
                     ISELAHDSTWKGPEDHMACRTQFDPATIWEIHVDPYGNVTTCCGIIIGNARRAPLAGL
                     LSGEGWLANPLVNVAYHEGPTGLLSMAVERGYVPRDGYPQKCGLCWEVRKFLRPHFPD
                     ILGPDEVYTVD"
     gene            complement(2436..>2700)
                     /locus_tag="PLQ54_10595"
     CDS             complement(2436..>2700)
                     /locus_tag="PLQ54_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013732753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93752.1"
                     /translation="DRNAWMGAPAGLLRADLSPKPAYDRLVGLIKGKWWTQTGGNTDD
                     AGQYAFRGFCGKYRIHVTDAGGAEVSADAHLERGADNAFIVKL"
BASE COUNT          452 a          874 c          914 g          460 t
ORIGIN      
        1 tcacgtgggg ctgggcgttc gggggccagt tctacgatga tgtgaaccgc aggatcacgt
       61 gcaccgaccc gaagatcgtc cgcgccctgg agtggatgac ctcctatgcc gatcggtacg
      121 acgtgaccaa gatcgccagc ctgcaggcgg ggttcgggac cgcggagcag aacccgttct
      181 acacgggacg cctcgccatg acgtgtctgc tgatcgcggg aatcgcggac atcgagcggt
      241 tcgcgcccga actggactac ggggtggcgc cgcagcccat gcccgatggc ggcgagaaag
      301 acagctcatg ggtcggcggc tggtgtatcg cgctccccag gggggcgcgc catcaggacg
      361 agggcttcga gttcatccgt tggctctgcc acgacccggg cggcacacag accgtgggcc
      421 gggagacggg cctcttcccc ggcatgaggc actccccgta cttcgatgag gtccgcaacc
      481 ggaagtacta cagcacgttt cttcggatcc tcgaggcgtg caagcaccag cggccagtca
      541 tgcccgtgca ggcgttctac atgcggcaga tgcagcgggc agtcgacagc gccatctaca
      601 agcagaagac ggccaagcag gcgctcgagg acgcggagaa ggccacgcaa gccgaactcg
      661 acctggtact gcgcgggggc gagtagggca cacatctgcc ccggccgcga gtcagccgct
      721 ccgcccggca agtatgggtc gccccaacat gcctagccgc cgtcgagcag gccgcgctcg
      781 ccaatgagga accagtcctc cgggtggtcg aggtagccca tggtctcctc gatgagcgcc
      841 aggtgccgac gctcctcatc ggcgaggaag gaccagaagg cccgggcttg gtcgtcggtg
      901 gcggctgatg cctgctcgcc gtagaactcg atgcccttct tctcgaagcc ggctgcctgg
      961 cgataagctt ccgtgtcatc gggcgccgcg gcggtggcgg tctcgccctc catggcctcc
     1021 cggaacatgg tcattacttc gccggagagg tcggagacac gggcggtgag actgaagtcc
     1081 ttccaggagc ccgcttgctt cagtgtggca atgtagtcgt tgatggcgtg gatgtggccg
     1141 atctcgtcct gcgccagagc tctgaacgtg gatgccacca gagggctctt ggtgttcgcc
     1201 gcgatggtct catagtactt gcggccgtcc acttcggtct tgagcgcgta ctccagggcg
     1261 ctaatagcct tctccgtgtt cacggacatg ctcgttatcc tctctcgatg gcatctgccc
     1321 ggtgtcgccc agctcggaga ccaacgcctg gccacaccgc caggttccgg ccggggccgc
     1381 tccctagtcc acggtgtaga cttcgtcggg gcccaggatg tccgggaagt gtggccggag
     1441 gaacttacgg acctcccagc acagcccgca cttctgaggg tagccgtccc gcggcacata
     1501 gccccgctcg acggccatgc tcaacagccc ggtgggcccc tcgtgatagg cgacgttcac
     1561 gagcgggttc gcgagccagc cttccccgga caggagaccg gccagggggg cccgacgtgc
     1621 gttgccgatg atgatgccac agcaggtggt gacattgccg tacgggtcga cgtggatctc
     1681 ccagatcgtc gccggatcga actgagtacg gcaggccatg tgatcctcgg ggcctttcca
     1741 ggtcgagtcg tgcgccagct cgctgatggg ccggtccggg aggtagcgcg cgagagcttc
     1801 cccggcgcgg ccgaccaggc atggcgggtt ggcgcggacg taatcggcca gacgttcttc
     1861 atcccgggca atggcctcga agtcgcggag ctcatccatg gtcagacgcg cggcggccac
     1921 gttccggtcg ccgaagacct cgacggccgc gcggaagcac cggtcgtagt tctcggcggg
     1981 gcactcatgc tggtggtggg ggcatgcact gatgagcagg cccaacactc cggcatcgcg
     2041 gagctgttcg agacgccggt aagccagcgc gtcgctgatg caccaggtgg cattcgtctc
     2101 gacgaaatgg ggtgcgaccc cttccctgcc ggcctcacgg cataggtcga tgagggcaga
     2161 ccagtacatc atggcctcac cgccggcgat gtggaccacc cggtcggtct gatggagccg
     2221 acggaggtac tcgaggccat cctcgaggcc gacatgcaca ttgggtttgc ggggactgca
     2281 actgaacagg cagtgacggc aggcgagagt gcagcggtac gtgtagagca cgctggcgac
     2341 atgcttcttc tggatcgcgg ccgccacgcc gtcctcgcgg atcgcctgcg ccaccgggac
     2401 ggcgacatac tcagtgcgca cgcccagtcg ccccctcaga gtttcacgat gaacgcgttg
     2461 tcggcgccgc gctcgaggtg ggcatcggca gacacctcag cccccccggc atcggtcacg
     2521 tggatgcggt acttgccaca gaacccgcgg aaggcgtact ggcctgcgtc gtcagtattg
     2581 ccgcccgtct gcgtccacca cttgcccttg atgagtccca cgagccgatc gtatgccggt
     2641 ttgggggaga ggtcggcacg cagcagacct gcgggggcgc ccatccacgc gttgcggtcg
//
LOCUS       DAOSPL010000554         2696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_37831_length_2696_cov_2.543355, whole genome shotgun sequence.
ACCESSION   DAOSPL010000554 DAOSPL010000000
VERSION     DAOSPL010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37831_length_2696_cov_2.543355"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="PLQ54_10600"
     CDS             complement(<1..877)
                     /locus_tag="PLQ54_10600"
                     /inference="COORDINATES: protein motif:HMM:NF026221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93753.1"
                     /translation="MDLTRCAIAALLLVAVPMSPVVADAAPAASAGIRAFCVDFKWWG
                     PGGFAPPGTFARADPEQHLAWYRALGANVIQTFCVSCDGYAWFRGRVAPVQPGMRGDF
                     LRELADLGHQHGMKVMGYFCVGANRYWGEQHPELSHGAPSAIHIPLTKEYLQYLCDTI
                     RDALRRTEIDGFMIDWVFNPEPEGEWLECERRMYVELFGKPFPGAAGVDAEEKVEFGR
                     RAVDRAWQRIRRAAKSAKPDCVIWLSSHDLANPQQKNSRLVREVDWLMTEGPSRELLD
                     VARAAVKLIQAGGLDI"
     gene            1191..1991
                     /locus_tag="PLQ54_10605"
     CDS             1191..1991
                     /locus_tag="PLQ54_10605"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS93754.1"
                     /translation="MKYSFMSFSCPDLELSPMLALARRLGYDGVEPRIDSGHRHGIEL
                     DASPAARKEARQLALDHGVALCCIATSCTYADPAKSGEAVELTRRAIDLAADVGSPRI
                     RVFGGPIPGGVSRKAAIDQVAHCLTTVADYAQACGVTVCMETHDDWCRPTDVAAVMRR
                     VERPAIAVNWDIMHPARTARVTIDDAFDALLPWIRHVHFHDGTIGSALDLRPMGRGEI
                     DHRRAVHLLQGAEWDGYLSGEWIDWEPYDVHLPRELAAMKAYEAEANR"
     gene            2214..2543
                     /locus_tag="PLQ54_10610"
     CDS             2214..2543
                     /locus_tag="PLQ54_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOS93755.1"
                     /translation="MKVAVASDGSQVSPHFGRCERYVLADVENGEVTALTVLENPGHE
                     PGVLPTLLHSHGVQVVIAGGMGPRAVGLFHDHGIEPVVGVSGPVDEVLARFVAGQLET
                     GPSACDH"
BASE COUNT          453 a          871 c          921 g          451 t
ORIGIN      
        1 tgatatccag cccgccggcc tggatgagct tgacggcggc gcgggcgacg tccagcaact
       61 ccctcgacgg gccctcggtc atcagccagt cgacttctcg caccaggcgc gagttcttct
      121 gttgcgggtt cgcaagatcg tgcgaactga gccagatgac gcagtcgggc ttggcgctct
      181 tggcggcgcg gcggatccgc tgccaggcac gatcaacagc tcggcggccg aactcgacct
      241 tctcctcggc atcgaccccg gcggccccag ggaatggctt gccgaaaagc tcgacataca
      301 tgcgtcgctc gcactcgagc cactcgccct cgggctcggg gttgaacacc cagtcgatca
      361 tgaacccgtc gatctccgtt cgccggaggg cgtcccgaat ggtgtcgcac aggtactgca
      421 gatactcctt cgtgagcgga atgtggatcg cgctgggggc gccgtggctc agctccgggt
      481 gctgctctcc ccagtagcgg ttggcgccga cgcagaagta gcccatgacc ttcatgccgt
      541 gttggtggcc gaggtcggcc agctcgcgca ggaaatcacc ccgcatgccc ggctgtacgg
      601 gcgcgaccct gcccctgaac cacgcatacc cgtcgcagga cacgcagaag gtctggatca
      661 cgttggcgcc gagagcgcgg taccaggcca ggtgctgctc cggatccgct cgcgcgaagg
      721 tgccgggtgg cgcgaagccc cccggccccc accatttgaa gtcgacgcag aaggcccgga
      781 tgcccgccga agctgcgggg gcagcgtcgg cgacgacggg gctcatcggc acggccacga
      841 gcaacaaggc ggcgatcgcg cagcgcgtga ggtccatggt cacatgcctc ccgggatcgg
      901 gccactgcgt tggccggcgt gttcgcccgg cggcgcgccc atgcctgccc ccgtggccac
      961 acaccagctc gtcgctggcg gcgacaaatc cgagacccaa atcggaacct ggatggcacc
     1021 gtggacaatg aacccggggc aagcgaacga atggtcagtc gcaggctcct cagagagggt
     1081 atgcttagcg atcgggtggc cggagcgagt actaccaatg tcttgggcgt gccgggtggc
     1141 ccaagccagg tcgcaggctg ctcgcgacct cgagccttgg gggaataccc ttgaagtact
     1201 ccttcatgag cttctcgtgc cccgacctcg agctcagccc catgctcgcc ctggcccgcc
     1261 gtctgggcta cgacggtgtt gagccgcgta tcgactcggg ccaccgccat ggcatcgagc
     1321 tcgatgcgag ccccgccgcc cgcaaggaag caaggcagtt agctctggac cacggcgtcg
     1381 cgctgtgctg catcgccacg tcgtgcacct atgcggatcc ggcaaagtcc ggcgaggctg
     1441 tggagctcac gcgccgggcg atcgacctgg ccgccgatgt cggctcgccg cgcatccgcg
     1501 tcttcggcgg cccgatcccc gggggcgtgt cgcgcaaagc ggcaatcgac caggtcgcgc
     1561 actgtctcac caccgtggct gactacgccc aggcatgtgg tgtcaccgtg tgcatggaaa
     1621 cgcatgatga ctggtgccgg ccgaccgacg tggctgcggt gatgcggcgc gtggagcgcc
     1681 cggccatcgc ggtcaactgg gacatcatgc atccggcgcg cacggccaga gtgacgatcg
     1741 atgacgcatt cgacgctctg ctgccgtgga tccgccatgt ccacttccac gacggcacca
     1801 tcggatcggc actcgatctg aggcctatgg gccggggcga gatcgatcat cgccgggccg
     1861 tccatttgct gcagggagct gagtgggacg ggtacctgag cggcgagtgg atcgactggg
     1921 agccctacga cgtccatctg ccgcgcgagc tggctgccat gaaagcctac gaggccgagg
     1981 cgaaccgcta ggcgattcgg cgggatcgtc gccgatccaa ccacagcagc gtcccgcggc
     2041 cctgaatacc gcctacgctc ccggtgcccg tgcattcgcc gtcatcggtt gacatgtatt
     2101 ccccgatgtg ctatatgttt gagacattta gtggccgcac gactgccatt cctgggcggc
     2161 caccccgatc gggaagcgcg cagagaccct tgggtggact ggagagtggt tgtgtgaagg
     2221 tcgcagtggc atcggatggg tcacaggtct caccacactt tgggcggtgc gaacggtatg
     2281 ttctggcgga tgttgagaat ggcgaggtca cggcactgac cgttctggag aaccccggac
     2341 acgagccagg ggtgctgccc acactgctcc actcccacgg ggtgcaagtc gtgatcgcgg
     2401 gcgggatggg tccccgggcg gtcgggctgt tccacgacca cgggatcgag ccagtggttg
     2461 gcgtttccgg gccggtggac gaagtgctgg ctcgcttcgt ggcagggcag ctggagaccg
     2521 gccccagcgc gtgcgaccac tagatggagg gcccctacca atcgatggca ttgggaatgg
     2581 aaaggagagg atcatcatga ggcacaggaa catgtactac gcgacgggct tgccgggttg
     2641 gatgcggttc ggttacagcc cgggttgggt cggacggagc ccgacgggcc tggggc
//
LOCUS       DAOSPL010000555         2695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_37842_length_2695_cov_3.670833, whole genome shotgun sequence.
ACCESSION   DAOSPL010000555 DAOSPL010000000
VERSION     DAOSPL010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37842_length_2695_cov_3.670833"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /gene="yidC"
                     /locus_tag="PLQ54_10615"
     CDS             <1..1216
                     /gene="yidC"
                     /locus_tag="PLQ54_10615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOS93756.1"
                     /translation="LLALGYIEMAQIREAAGDYDAKLDYEKAAQTYEQFAAQHRASPW
                     AAHATYWAGVIYGRHVSQGDDRHRKKMVKLLGGLAFEHRSVWVPSDDGSGMWEERRAL
                     IESGKAVEPYVNSRFGYRLLDTLVRICGGAKQPLSYVMALLLVSIIVKVVSLPLAIRG
                     QVAMKTMQAKMARIQPQIEEIKKKYANDQMKAMQEQSKLMKESGISMMGGCLPQLIQL
                     PFIFYLYYAIRLYSYHFENGSFLWITDLSRPDLPLLVIYAVSMIASTKLTPQPPATDP
                     QQQNTQRMMTYMMPAFLFLVLRTFPAAFIFYWAAFNVLGTAQQVWMNKAYPGPKPEDL
                     GPGRHGAPAPARRLSSGGSGWLERFVTKREDGRGNGPVIDAPARGNKLSSGKGRAAAR
                     RKPSIPPPKSRS"
     gene            1299..1778
                     /gene="jag"
                     /locus_tag="PLQ54_10620"
     CDS             1299..1778
                     /gene="jag"
                     /locus_tag="PLQ54_10620"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOS93757.1"
                     /translation="MSDIETSASTAAETAREALQGILDRMGIECTVSAHVDGEGQITL
                     DVSSDSDLGTVIGRDGDTLNALQLLVSVIVSRRHGPHSRVVVDAENYRARRAESLQAR
                     AIELAQRVKATGREALLESLHAYERRIIHLTLADHPDVYTYSEGEGDARVLVISPRD"
     gene            complement(1793..>2695)
                     /locus_tag="PLQ54_10625"
     CDS             complement(1793..>2695)
                     /locus_tag="PLQ54_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS93758.1"
                     /translation="VQPEVGLEALAGMDQQKDLLLRVAQAMREGHRRVVPQGIMLLGP
                     PGCGKTFCMECFAHDCGIPFLRLGNIFSRYVGATEANLEKLLHCLNALSPVFVFIDEF
                     DQSYGRRVTSEGDSGVSRRVFAMFNAYLSDSSRQGRVLIGAASNRPDLIDPSTLRAGR
                     FDLKIPFFLPDDATRSQILEVTYRTLRIDHRIEDFAGPAQATRGYSGADLRELVLQSF
                     RLATFEGRRRVMQRDLDAAVADYIPPVRSDPEMVRFMELLAVSGTTSKRLLSPEHQAL
                     IDSGRLQAEIDDLRQRVSQRGATM"
BASE COUNT          499 a          847 c          902 g          447 t
ORIGIN      
        1 actgctggcc ctggggtaca tcgagatggc ccagatccgc gaggccgccg gtgactacga
       61 cgcgaagctc gactatgaga aggcggcaca gacctacgag cagtttgccg cccagcaccg
      121 agcgagtccc tgggccgccc atgccacgta ctgggccggt gtgatctacg gtagacacgt
      181 gtcccagggg gatgaccggc accggaagaa gatggtgaag ctcctgggcg ggctggcgtt
      241 cgagcatcgg tcggtgtggg ttccctcgga cgacggttcg ggaatgtggg aggaacgccg
      301 cgcgctgatc gagtcgggca aggccgtgga accctacgtg aactcgcgct tcggctatcg
      361 cctcctcgat accctggtgc gcatctgcgg cggcgcgaag cagccacttt cctatgtgat
      421 ggcgctactg ctggtgtcca tcatcgtgaa ggttgtctct ctcccgttgg ccatcagggg
      481 ccaggtcgcg atgaagacca tgcaggccaa gatggcccgc attcagcccc agatcgagga
      541 gatcaagaag aagtacgcca acgaccagat gaaggccatg caggagcagt ccaagctgat
      601 gaaagagagc ggcatcagca tgatgggcgg ctgcctgcct cagctcatcc agctcccctt
      661 catcttctac ctctactacg cgattcggct atattcgtac cactttgaga acggcagttt
      721 cctttggatc accgatctgt cgaggccgga cttgcccctg ttggtgatct acgcggtgag
      781 catgatcgcg tcgaccaagc tgacgccgca accgccggcc accgacccgc agcagcagaa
      841 cacgcagcgg atgatgacct acatgatgcc ggccttcctg tttctggtgc tgcggacgtt
      901 cccggcggcc ttcatcttct actgggcggc gttcaacgtg ttgggaacgg cgcagcaggt
      961 gtggatgaac aaggcgtatc ctggcccgaa gcccgaagac ctgggaccgg gccggcacgg
     1021 ggcgccggca ccagcccgtc gcctatcgag cggcggctca ggttggttgg agcggtttgt
     1081 caccaagcgc gaggacggtc gcggcaatgg accggtgatc gatgcgccgg cacgagggaa
     1141 caagcttagc agcggcaagg gacgagccgc cgcacgccgc aagccatcca tcccgccccc
     1201 caagtccaga tcctaaccgg ggcgtgggtg gtttgggcca cgcccctcgc agggcgctgg
     1261 cgattccgca cgatcgagag cacaggggcg actgagcatt gagtgacatc gagaccagtg
     1321 cgagcacggc ggctgagacg gcccgggaag cgctgcaggg gatactggac cgcatgggca
     1381 ttgaatgcac cgtctcggcc cacgtcgatg gcgagggcca gatcaccctc gacgtatcct
     1441 cggactcgga cctgggtaca gtcatcggtc gggacggcga tacgctgaat gcactgcagc
     1501 tgctggtgag tgtgatcgtt agccggcgcc acggccccca ctctcgggtg gtggttgacg
     1561 cggagaacta ccgggcgcgg cgtgccgagt ccctccaggc tcgagccatc gagctggcgc
     1621 agcgcgtgaa ggcgacgggc cgggaggcgt tgctcgagtc actgcatgcg tacgaacggc
     1681 ggatcatcca cctgacgctg gcggatcacc cggatgtgta cacatacagc gagggtgaag
     1741 gcgacgcccg ggtcctcgtc atctcgccta gagactagct ggggccgcac ccctacatcg
     1801 tcgcgccgcg ctgtgagacc cgctggcgaa ggtcatcgat ctcggcctga agccggccgc
     1861 tgtcgatgag tgcctggtgc tcgggtgaga gcagtctctt cgacgtcgtc cccgacacgg
     1921 ccagcagctc catgaaccgc accatctccg ggtccgaccg gaccggcggg atgtagtcgg
     1981 ccaccgcggc atccaggtcg cgctgcatga cccgtcgccg accttcgaat gtggccagcc
     2041 ggaacgactg gagcacgagc tccctcagat ccgccccgga gtagccgcgg gttgcctggg
     2101 ccggaccggc gaagtcctca atgcggtgat cgatcctcag cgtcctgtaa gtgacttcga
     2161 ggatctggga ccgcgtggcg tcatccggca ggaagaaggg gatcttgagg tcaaaccgcc
     2221 ccgcccggag tgtggatggg tcgatgaggt ccggccggtt cgaggccgca ccgatgagca
     2281 cgcggccctg tcggctcgag tccgacagat acgcgttgaa catcgcgaag acgcggcgcg
     2341 agacgccgct atcgccctca ctagtcacgc ggcgcccgta cgactggtcg aactcgtcga
     2401 tgaagacgaa aacgggcgag agcgcattca gacagtggag cagcttctcc agattggcct
     2461 cggtagctcc cacgtagcgc gagaagatgt tcccgagccg gaggaacggg atcccacagt
     2521 cgtgggcgaa gcactccatg cagaatgttt tcccgcagcc gggtggcccg agaagcatga
     2581 tgccctgcgg aacgacgcgg cggtgcccct cgcgcatggc ctgggccact cggagcagga
     2641 ggtccttctg ctggtccatc cccgccaggg cctcgaggcc gacctcgggc tgcac
//
LOCUS       DAOSPL010000556         2693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_37879_length_2693_cov_2.587566, whole genome shotgun sequence.
ACCESSION   DAOSPL010000556 DAOSPL010000000
VERSION     DAOSPL010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37879_length_2693_cov_2.587566"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLQ54_10630"
     CDS             complement(<1..346)
                     /locus_tag="PLQ54_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93759.1"
                     /translation="MVTADIGMYILVGLIVGATNGIPYVGTALVCGSFLAIFASLQTG
                     SRLELSPLGRAFQADIGLPAFLVALIQGGIVFVIYIAMFVIGLITGGLGLIVMIPGLL
                     VVILGMGVLWLFA"
     gene            complement(425..1807)
                     /locus_tag="PLQ54_10635"
     CDS             complement(425..1807)
                     /locus_tag="PLQ54_10635"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOS93760.1"
                     /translation="MGAEGVVYLSEAMVRSSLMEPELRQVANVACLPGLQGPSMAMPD
                     IHFGYGFPIGGVAATDSEAGVISPGGVGYDINCGVRLLRTDLTRAEIEPVLDRLLHQI
                     SRDVPSGVGSEVRDKLAKTDLERIAQRGARWAVERGFGTAADLEVTEENGALAGADPA
                     AVSARAVERGLKQVGTLGSGNHFLEIQAIDEIYDAQAAAAMGLHEPGQITVLIHTGSR
                     GYGYQICDDSIEMMQRAVVKHRISLPDRQLACAPVSSSEGTQYLSAMACAANFAWANR
                     QMIAHRVRGVFERILGQSSDALGMHQVYDVAHNIAKIEEHPVGGAVRKLCVHRKGATR
                     AFAPHTPGVPARYQEIGQPVLVPGDMGRYSFVLVGTDVAMWETWGSTCHGAGRLLSRA
                     AAMKLRPGHEVIAHMRDRGVLVVTPNKRGVAEEQPEAYKDVGDVVDVVHGAGLSTKVV
                     RMRPLGVVKG"
     gene            complement(1946..2521)
                     /gene="rplI"
                     /locus_tag="PLQ54_10640"
     CDS             complement(1946..2521)
                     /gene="rplI"
                     /locus_tag="PLQ54_10640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOS93761.1"
                     /translation="MRVVLREEIEGLGYPGDCVRVRDGYGRNYLIPRGLAVLATPGAL
                     RAVEQAKEQRRRVESRARDQFEKLAATLAGKTVRVEKRAGSGMKLYGSVTTADIAEAL
                     KAQYGLDIERRRLELESQIKTVGTHEVTARVSADVHAKFLIEVVREGGEPVAEEAAAA
                     TEAPEVVEAPEAPAADAPAAEEPAIEDSVDS"
BASE COUNT          459 a          999 c          823 g          412 t
ORIGIN      
        1 aggcgaacag ccagaggacg cccatcccaa ggatcacgac cagcaggccc gggatcatga
       61 cgatgagacc caggcccccc gtgatgaggc cgatgacgaa catggcgatg tagatcacga
      121 agacgatgcc gccctgaatg agcgcaacca ggaaggccgg cagcccgatg tccgcctgga
      181 aggcgcggcc gagcgggctg agctcgagcc gggacccggt ctgcaggctc gcgaaaatcg
      241 cgaggaagga accacagacc agggccgtgc cgacatacgg aatcccattg gttgcgccga
      301 cgatcagacc gaccagaatg tacataccaa tgtcggccgt caccaccgac cacgcctcgc
      361 gcagccagtc gccagtgcgg accatagata ccacccccgc cctgcatatt gcgcggcggg
      421 cctactatcc cttcacgaca cccaggggcc tcatgcgaac caccttggtc gagagccccg
      481 cgccgtgcac cacgtcgacg acatccccca cgtccttgta ggcctccggt tgctcttcgg
      541 ccacgcctct cttgttcggc gtgaccacca gcacgccgcg gtcccgcatg tgggcgatga
      601 cctcgtgccc cggccggagc ttcatcgccg ccgcgcgcga aagcagccgg ccggctccat
      661 ggcaggtgga cccccacgtc tcccacatgg ccacgtccgt gccgaccagc acgaaggagt
      721 agcggcccat gtcgcccggc accagcacgg gctgaccgat ctcctgatac cgggccggca
      781 ctccgggcgt gtgcggcgca aaggcccgtg tggcaccctt gcggtgcacg cagagcttcc
      841 gcacggcacc gcccaccggg tgctcctcga tcttcgcgat gttgtgcgcc acgtcataca
      901 cctggtgcat tcccagcgcg tcggagctct gcccgaggat cctctcgaaa acaccccgga
      961 cccgatgggc gatcatctgc cggttcgccc aggcgaagtt agccgcgcac gccatggccg
     1021 acagatactg tgtcccctcg ctcgatgaga ccggtgcgca cgccagttgg cggtcgggca
     1081 gcgagatcct gtgcttcaca acggcccgct gcatcatctc gatcgagtcg tcgcagatct
     1141 gatagccgta accccgcgat cccgtgtgga tcagcaccgt gatctggccg ggctcgtgca
     1201 accccatggc cgcggcggcc tgcgcgtcat agatctcgtc gatggcttgg atctcgagga
     1261 aatggttacc ggagcccagt gtaccaacct gcttgagccc gcgctcgacg gcccgcgccg
     1321 agacggccgc gggatccgct cctgcgagag ccccgttctc ctccgtgacc tcgaggtcgg
     1381 cggccgttcc gaagccgcgc tcgaccgccc aacgggcgcc gcgctgggcg atccgctcca
     1441 ggtcggtctt cgcgagcttg tcgcggacct ccgaccccac tcccgacggc acatcgcgcg
     1501 agatctgatg gagaaggcgg tcgagaaccg gctcaatctc cgcccgggtc aggtcggtgc
     1561 ggagcagacg aacgccgcag ttgatgtcat accccacacc gccgggcgat atgaccccgg
     1621 cttcggagtc cgtggccgcc acgcctccga tgggaaaacc gtagccaaag tgaatgtcgg
     1681 gcatggccat cgacggcccc tgaaggcccg gcaagcatgc cacattggcc acctgcctga
     1741 gctcgggctc catcagcgac gaccgaacca tggcctcgct aaggtacacc acaccctcag
     1801 cgcccatgcc caactgcgga tcgcggggga cgcgccagcg atagtcatcg actggctcta
     1861 gtgtgatgct gcccacaact ctcggttcca cgaccgcgcc ctccctcccc ggagccgcgc
     1921 ggctgggccg ccaggctgcg ggtccttacg agtccaccga atcctcgatg gccggctcct
     1981 cagcggcagg cgcatcggcc gcaggtgcct ccggcgcctc gaccacctcg ggggcctccg
     2041 tcgcggccgc ggcctcctcg gccacgggct cgcccccctc gcggacgacc tcgatgagga
     2101 acttggcgtg aacgtcggcc gacacccgcg cggtcacctc gtgggtgccc accgtcttga
     2161 tctgcgactc caactcgagg cgccggcgct cgatgtcgag cccatactgc gccttgagcg
     2221 cctccgcgat atcggcggtg gtgaccgagc cgtacagctt catgcccgag ccagcgcgct
     2281 tctcgacgcg aacggtcttg cccgccagag tggcagcgag cttctcaaac tggtcgcgag
     2341 cgcgtgactc gacgcgacgc cgctgttcct tggcctgctc gaccgcgcga agcgcccccg
     2401 gcgtcgccag cacggccaga ccacggggaa tcaggtagtt gcgcccatac ccgtctcgga
     2461 cacgtacaca gtcacccggg tagccgagcc cctcgatctc ttccctgagt accactctca
     2521 ttgcgatatg cctccatacg cttagggccg gccgctcgag cgcagcgcca agcagcgtgc
     2581 attcccgtgg ggcgccccgc ctgccgggcc gcacacgagg cccagcgtct ggtggcgacg
     2641 gacgcaatct cacagtttac cgattcccgc cgcgcgcgtg aagagcccgt ttg
//
LOCUS       DAOSPL010000557         2693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_37881_length_2693_cov_2.344200, whole genome shotgun sequence.
ACCESSION   DAOSPL010000557 DAOSPL010000000
VERSION     DAOSPL010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37881_length_2693_cov_2.344200"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLQ54_10645"
     CDS             complement(<1..391)
                     /locus_tag="PLQ54_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93762.1"
                     /translation="MTRDRIAAVIVLAAVVCVLGVGLAQDPHPGPPRDLDSQLRDIRG
                     PVQYVGPVPWGRIVLIAAGLLALAALVGLLAVVPARRRASRPVPTAKAPETAGDRAVR
                     RLQVLATSGLWERGEYARFADQLSSILR"
     gene            complement(388..1353)
                     /locus_tag="PLQ54_10650"
     CDS             complement(388..1353)
                     /locus_tag="PLQ54_10650"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOS93763.1"
                     /translation="MTRSSTGLWRRSRSREPDSGRGTSGPGTPQLSDILDRVRRLELK
                     TQRLVDSLFAGQYRSAFRGHGLEFRDVREYDYADDVRLIDWRVTARTGVAHVRQYDEE
                     RELTIVCCVDISPSSAFGTTRGLKRDVAAELAAYIGFSAARSNDRFGCLLFSDRVEEL
                     VPPRKGRRHVLRIVRDALAFEPTGRGTSISAALRAVHQAVHKRCVLIVVSDFMDRDFE
                     HDLRVASLRHDAVAVELVDPREYELPSVGLVELVDPETGATQIVDSSDGAVRSAFAAS
                     AVEARQRRLDLLRSAGVDVLSLRTDQEMFGPLMRFFRSRRRWTRR"
     gene            complement(1307..2233)
                     /locus_tag="PLQ54_10655"
     CDS             complement(1307..2233)
                     /locus_tag="PLQ54_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS93764.1"
                     /translation="MERLSQEIGKVIVGQSQLVRSLLIAMLTNGHVLLEGVPGLAKTL
                     AVRTLADAISARFQRIQFTPDLLPSDLIGSVIYNQQAAEFVVRRGPIFANLVLADEIN
                     RAPAKVQSALLEAMQERQVTIGQQTHALPDPFVVLATQNPIEQEGTYPLPEAQVDRFM
                     LKVVVGYPSRAEEIAIVTRMAGPSEPKAEPVVDLGFIAEARDTVRRIYVDERVTAYVV
                     DIVTATRSVQGDRGAELVEYGASPRASIWLTHAAKAVAFLNQRGYVVPADVKEVARDV
                     LRHRVIVTYEAEAEGITSDQIIHRALASVKVP"
     gene            complement(2465..2693)
                     /locus_tag="PLQ54_10660"
                     /pseudo
     CDS             complement(2465..2693)
                     /locus_tag="PLQ54_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019265221.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="disulfide reductase"
BASE COUNT          480 a         1009 c          795 g          409 t
ORIGIN      
        1 cccggaggat actggacaac tgatccgcga aacgcgcgta ctcgccccgc tcccacagac
       61 ccgacgtcgc cagcacctgg agccggcgca cggcacggtc gcccgccgtc tccggcgcct
      121 tcgcggtagg caccgggcgc gacgcgcgcc gacgcgctgg aacgacggcc agaagcccca
      181 ccagtgccgc cagcgccagc agaccggccg cgatcagcac gatacgaccc cacggcacgg
      241 gccccacata ctgcaccgga ccccgtatgt ctcggagctg gctatccagg tccctgggcg
      301 gcccggggtg cgggtcctgc gcgagaccca cccccagcac acagaccaca gccgcgagga
      361 cgatgaccgc cgcgatgcga tcgcgagtca tcgacgggtc cacctccgtc gcgaacggaa
      421 gaagcgcatg agcggcccaa acatctcctg gtcggtgcgc agcgaaagca catccacacc
      481 cgccgaacgc agaagatcca gccgacgttg tcgggcctca actgccgacg ctgcgaaggc
      541 cgagcggacc gcgccatccg acgagtccac gatctgcgtc gcaccggtct ccgggtcgac
      601 cagttcgacg agccccacgc tgggcagctc gtactcgcgc gggtccacca gctccacagc
      661 gaccgcgtca tggcggagcg acgccacgcg cagatcgtgt tcgaagtccc ggtccatgaa
      721 atcggacacg acgatcagca cacaccgctt gtgaaccgcc tggtgtaccg cccgcagggc
      781 cgccgagatc gaggtgcctc ggcccgtagg ctcgaacgcc aaggcatcgc gcacgatgcg
      841 gagcacgtgc cgccgtccct tgcgaggtgg caccagctcc tccacccggt cggagaacag
      901 cagacacccg aaccggtcgt tgctgcgcgc cgccgagaac ccaatgtagg cagccaactc
      961 cgccgccacg tctcgcttca aaccccgagt ggtcccaaac gccgacgatg gactgatgtc
     1021 gacacagcac acaatggtga gctcgcgctc ctcgtcatac tgccgtacgt gcgctacccc
     1081 ggtccgtgcc gtcacccgcc agtcgatgag ccgcacgtcg tccgcgtagt catactcccg
     1141 cacgtctcgg aactcgagcc catggccgcg aaacgcactc cggtactgcc ccgcgaacaa
     1201 actgtccacc aggcgctgtg tcttcagttc gaggcgccga acacgatcga ggatgtctga
     1261 gagctggggc gtgcccggac ccgacgtccc ccggcctgag tccggctcac gggaccttga
     1321 ccgacgccag agcccggtgg atgatctggt cactcgtgat cccctccgcc tccgcctcat
     1381 aggtcacgat gacacgatgg cgcaggacat cgcgcgcgac ctccttgacg tcagcgggca
     1441 ccacatagcc gcgctggttc aggaacgcca cggccttggc cgcgtgggtg agccaaatcg
     1501 aggcccgcgg cgacgcccca tactccacca gttcggcgcc ccggtcgccc tgcaccgacc
     1561 gcgtcgccgt cacgatgtcc accacatagg ccgtgactcg ctcgtctacg tagattcgcc
     1621 ggaccgtgtc ccgcgcctcg gcgatgaacc ccaggtcgac caccggctcc gccttgggct
     1681 ccgaaggccc cgccatacgc gtcacaatcg cgatctcctc ggcccggctc gggtagccca
     1741 caaccacctt cagcatgaac cggtcgacct gtgcctcggg cagcgggtac gtgccctcct
     1801 gctcgatcgg gttctgggtc gccaacacca caaacgggtc gggcagggcg tgtgtctgct
     1861 gcccgatggt cacttgtcgc tcctgcatcg cttccagcag cgcgctctga accttggcgg
     1921 gtgcccggtt gatctcgtcg gccaacacca gattggcgaa gatggggccg cgccgaacca
     1981 cgaactccgc cgcctgttgg ttgtagatca ccgacccaat caggtcactc gggagcaggt
     2041 ccggagtgaa ctgtatgcgc tggaaacgcg ccgagatcgc atccgccaac gttcgaaccg
     2101 ccagcgtctt cgctaacccc ggcacacctt ccagaagcac atgcccattg gtaagcatgg
     2161 cgatcagcag actgcgcacc agctgagatt gacccacgat gaccttgcca atctcctgcg
     2221 acagccgctc catcctgccc gctgccgcgg cggcctgccg gccgatcgct tccacctcgg
     2281 catgctccaa agccgatcgt tccctccaaa cagcaccgac ctatggggtc cgctcctgcc
     2341 cactgcggag cgtctgggca ccgcaccgat ggaccacgcc ccaggtcagg gccagatgct
     2401 gctgcatctc ctcggccgtc ggggcatagg ccgcgaactt cacgcgctcc gccgccgccg
     2461 acaccagcga cttggccttc tcggtggccg ccgccttgtc cttggccacc cacgagacgt
     2521 gctcgcggac attggccatt tcgaacaggt aggggttcat gcccgcgttc tggcacgcgc
     2581 ggcggaacgt cttctcgtgc aggtggggcg aacaggccgc cacgaccacc cgggtcagat
     2641 tctggttctt gatgtcgtct tcgatcatct gctgaccgag cgacgagcac atg
//
LOCUS       DAOSPL010000558         2689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_37937_length_2689_cov_3.752468, whole genome shotgun sequence.
ACCESSION   DAOSPL010000558 DAOSPL010000000
VERSION     DAOSPL010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37937_length_2689_cov_3.752468"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PLQ54_10665"
     CDS             <1..913
                     /locus_tag="PLQ54_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93765.1"
                     /translation="CCTVEEVNSAVEAAAAAFQRSEVPVEAKPSTEAIQVADIVLSPL
                     SAAGNTYLPIAPGNEWEYGLTRTLDGDVAIGPLADLVGPIEFKGTMLRSVGASDDKRI
                     PNITLFPIEEEETVFVSGESSEGSTERRFNRDFPVELARDGVVARLGTDAELHQWLPS
                     NVASGSTWVGWVPVGPYRVIGSAKATTVPSLAVPAGEFENVLRVTLTPTQVTGDGIDS
                     SQVTGEVSMCFAPGVGEIQREVKLKVQVSGASPKSGESLSGAIDIVESLDLKSYKLAQ
                     AAAPAAPPVPAPSPVPAPEGGHPAPNE"
     gene            943..1275
                     /locus_tag="PLQ54_10670"
     CDS             943..1275
                     /locus_tag="PLQ54_10670"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOS93766.1"
                     /translation="MQRVNEHDLEYRHGDHGPKYLFRGPRHEWGIIRLGPGHEVDLHH
                     HKEVQETFYFEEGAPTLILNGEALRVGPGDAFQLDPGETHRIINDTAEPVRIIFIKTP
                     YLPEDKVS"
     gene            complement(1453..2385)
                     /locus_tag="PLQ54_10675"
     CDS             complement(1453..2385)
                     /locus_tag="PLQ54_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93767.1"
                     /translation="MQTRWRPLWMVAALVSVCGVCASQPDEAAEAAPTRLRRREPIRF
                     NPPPDCEHRFACAVSGQLKASFWGRHRVRGEAVMLRKVLEVDPARDLVQVSTGLESGI
                     CRVDDTAKQVGGEPPLICDFDARHRLLRVSKSGDPAGTEEVASASILDLGSVMMFLFY
                     SVPFHVRTAGVGTAWDAEYSCVDVNGDAVTLSGSHELIAFMFEGGRRVAVIRSDLQIP
                     VYGKINRYRLSGTLQCKVISEVYVDCGGLRYRDSVGAGTLKASGGIISVDVDVSDLRN
                     TLRLILPNGSPAEPLYDPTHPPADPTGGGQVPQS"
     gene            complement(2427..>2689)
                     /locus_tag="PLQ54_10680"
     CDS             complement(2427..>2689)
                     /locus_tag="PLQ54_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93768.1"
                     /translation="ESKLQIPVTGNVAGAHLIGRLECHVLSEVYVDDGSLRYRDMLAE
                     GTLAAGTADVEVDDLHTTLAVIPPKGDAARALYTAPDAADED"
BASE COUNT          463 a          916 c          891 g          419 t
ORIGIN      
        1 gtgctgcacg gtggaagagg tcaattctgc tgtggaagcc gccgcggcgg ctttccagcg
       61 atccgaggtg cccgtcgagg ccaagccgtc gaccgaggcc attcaggtcg ccgatatcgt
      121 cctcagccca ctgtcggccg caggcaacac ctacctgccc attgccccgg ggaacgaatg
      181 ggagtacggc ctgacccgca ccctggacgg tgatgtcgcc atcggtccgc tggctgatct
      241 ggttgggccc atcgagttca agggcacgat gctccggtcg gtgggggcgt cagacgataa
      301 gcgaatcccg aacattacgc ttttccccat cgaggaagag gaaacggtgt tcgtctccgg
      361 agagtccagc gagggctcga ccgagcggcg gttcaaccgt gacttcccgg tcgagctcgc
      421 ccgcgatggc gtcgtggcgc gcctgggcac ggacgccgaa ctccatcagt ggctgcccag
      481 caatgtggcc tccggctcga cgtgggtcgg ctgggttccc gtggggccct atcgggtgat
      541 cggctccgcc aaggccacaa cggtgcccag cctggcggtg cccgctggcg agttcgagaa
      601 cgtgctcagg gtcacgctca cgcccactca ggtgaccggc gacggcatcg actcgtcgca
      661 agtgactgga gaggtctcca tgtgtttcgc cccgggcgtg ggcgagatcc agcgcgaggt
      721 gaagctcaag gtccaggtca gcggagccag cccaaagagc ggcgagtcgc tatcgggtgc
      781 gatcgacatc gtcgagagcc tcgacctgaa gtcctacaag ctcgcccagg cggctgcccc
      841 ggcggcgccg ccggtacctg cgccttcacc cgtgccggct ccggaaggcg gccaccccgc
      901 gccgaacgaa taggcccgtc cacctgagcc cggaggactg ccatgcagcg cgtgaacgag
      961 catgacctcg agtaccgcca cggcgaccat ggaccgaagt atctgttccg aggcccgagg
     1021 cacgagtggg gcatcatccg cctgggcccg gggcacgagg tggacctcca ccaccacaag
     1081 gaggtccagg agaccttcta cttcgaggag ggcgctccga cgctcatcct caacggcgaa
     1141 gcgctccgcg tcggcccggg cgatgccttc cagctcgacc cgggcgagac ccaccggatc
     1201 atcaacgaca ccgccgagcc tgttcgcatc atcttcatca agactccgta cctgccggag
     1261 gacaaggtga gctaggtagt cctgcggcga acggcggcac ggacgtgaac gcggggcact
     1321 gagcccttcg ccgcggcagg tgctgagtcg gcgtgaggcc tcactgggca aggaagtccg
     1381 tgcccatcgt cgcggccacg actccacggg attgctcgct tggtccggga tggtgccagc
     1441 gaccgcgcgc cgtcaactct ggggcacctg gccgcctccc gtcggatcgg cgggcggatg
     1501 agtcgggtcg tacaggggct cggccgggct gccgttgggc aggatcaggc gaagcgtgtt
     1561 gcggagatcg gacacgtcga catcgaccga aatgatgcct ccgctggctt tcagggtgcc
     1621 agcgcccacg gagtcccggt agcgcaggcc gccgcagtcg acgtacacct cgctgatcac
     1681 cttgcactgc agcgtcccgg agaggcggta cctgttgatc ttgccgtaga caggaatctg
     1741 caagtccgag cggatcacgg ccactcgccg gccgccctcg aacatgaagg cgatcagctc
     1801 atggctcccc gacagcgtca ccgcatctcc gttcacgtcg acacaggagt actccgcgtc
     1861 ccaggccgtg ccgacaccgg cggtgcgcac atggaagggt acggagtaga acaggaacat
     1921 catcaccgag cccagatcga ggatgctagc cgaagcgacc tcttccgtgc cggccgggtc
     1981 gccggacttg ctgaccctga gcaaccggtg ccgtgcatcg aaatcgcaga tgagcggtgg
     2041 ctcgccgccg acctgcttcg cggtgtcgtc gacacggcag atgccggact cgaggcctgt
     2101 ggagacctgc accagatccc gagccggatc gacctcgagt accttgcgaa gcatcacggc
     2161 ttcgcctcgg acccggtggc gcccccagaa cgatgccttg agctgcccgg agaccgcgca
     2221 cgcgaagcgg tgctcgcagt cgggtggcgg gttgaaccgg atgggctcgc gtcggcgcag
     2281 ccgcgtcggc gccgcctcag ccgcctcgtc gggctggctg gcacagacgc cgcacacgga
     2341 caccagggcc gccaccatcc atagtgggcg ccatcgagtc tgcatcgggc tcacccctct
     2401 gctatgggac ggaccgcagc tcacgactag tcctcatcag cggcatcggg cgccgtgtat
     2461 agagccctcg ccgcgtcccc cttcggcggg atgacggcca gcgtcgtgtg cagatcatcc
     2521 acctcgacat cggccgtgcc cgccgccaaa gtgccctcgg ccagcatgtc gcggtacctc
     2581 aggctgccgt catcgacata gacctcactg agcacgtggc actcgagccg gccgatcagg
     2641 tgggcgcccg ccacgttacc ggtgacgggg atctgcagct tggactcga
//
LOCUS       DAOSPL010000559         2689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_37940_length_2689_cov_3.399013, whole genome shotgun sequence.
ACCESSION   DAOSPL010000559 DAOSPL010000000
VERSION     DAOSPL010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37940_length_2689_cov_3.399013"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1125
                     /locus_tag="PLQ54_10685"
     CDS             229..1125
                     /locus_tag="PLQ54_10685"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS93769.1"
                     /translation="MNRREFLGASAGALAGIGLGAGLTAQAEAAAAGYTLPAGRRKWR
                     KAMVAGMLPGDMSLDERFGLAKRAGFDGIEVPPMSEAEAAENREAAERNGLRIHSVIY
                     GGWGAPLSSGDDAVVTQGLKEVEGALRAARVLRADAVLLVPAVVNEGTSYPDAYERSQ
                     RNIRKLVPLAKELGVVIGIENVWNNFLLSPVEFARYVDEFESPHVRAYLDLGNMVKFG
                     WPEHWVQALGPRIAKVHLKDFRREGNQFVNLREGDVNWPLVTDWLVRVGYEGWFTTEL
                     GGGDEAYLKDVADRVDAIFAGK"
     gene            complement(1323..1973)
                     /locus_tag="PLQ54_10690"
     CDS             complement(1323..1973)
                     /locus_tag="PLQ54_10690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOS93770.1"
                     /translation="MLPDDHRLLERLRTGDRQAPVEIVDRHMRDAYDFLWMLTQDRET
                     AADLTQETMVQVWRALGRFRGECRLSTWIRKIALNQYRQHLRRTQRRGAELECEAPDV
                     ASPFDLAQSVVDADSRRLVRNAVAALPTIYREVVVMHCYGEASLKDVAQALGIPLGTV
                     KWRLSHAVQTLRARLAVALCEDQTRGTTDGRTRSAADRPTPTLDEPKRRLFVRPGS"
     gene            2132..>2689
                     /locus_tag="PLQ54_10695"
     CDS             2132..>2689
                     /locus_tag="PLQ54_10695"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOS93771.1"
                     /translation="MDRKLTRREMMAVGALGIAGVAARGGAVSEGDGRRKAMSLSLNS
                     SVIRPTPLERKVRIAAEMGYDGIELWDEELERYQGEGHSLTDLGHRIRDLGLFVPNII
                     GLWDCMPPEESQRASAVEVAKRRMERGVAVGARCIAAIPAPDREDIGIEWAAERYREL
                     LEVGREMGIRVAVEFVGFLRGVHRLG"
BASE COUNT          420 a          781 c         1022 g          466 t
ORIGIN      
        1 ttcggggcgg ccgatggcat cggcgtggcc atcgcgctgc tggctcttga ggcggacggt
       61 cgggcggttg tcttgtcttg cgactggtcg ggcgcgtgcg gggctcttgt ggtccgtcgc
      121 cggtaggtgg acagcagggg actcgccatt gggaacactg aaggctatgg cggcgcgatg
      181 tggtgggtgc gccgccgcgg agtcgcggga tctgggagga gttcatcgat gaatcgacgg
      241 gagttcctgg gggcttcagc gggcgccctc gcgggtatcg ggctgggcgc gggccttacc
      301 gctcaggcgg aggcggctgc ggcaggttac acgctaccag ccgggcgccg caagtggcgc
      361 aaggccatgg tggcgggcat gctgccgggt gacatgagtc ttgacgagcg ttttggtctg
      421 gccaagcgtg cgggtttcga tggcattgag gtaccgccca tgagtgaggc cgaggctgcg
      481 gagaaccggg aggcggcgga gcggaacggt ctgcgcattc actcggtgat ctatggtgga
      541 tggggggcgc cgttatcgtc gggtgacgac gcggtggtga ctcaaggcct caaggaggtc
      601 gagggggcgc ttcgcgcggc gagagtgctg cgcgcggacg cggtcctcct ggtccccgcg
      661 gtggtcaatg agggcacgag ctacccggat gcgtatgagc ggtcgcagcg gaacatccgc
      721 aagttggtgc cgctggccaa ggagttgggt gtggtcatcg gcatcgagaa cgtctggaac
      781 aacttcctgc tgagtccggt ggagttcgcc cggtacgtgg acgagtttga gagcccgcat
      841 gtccgggcgt acttggacct gggtaacatg gtcaagttcg gctggccgga acactgggtg
      901 caggctttgg gcccgcgcat cgccaaggtg cacctgaagg acttccgccg tgaggggaac
      961 cagttcgtga acctacgcga gggcgacgtg aactggccgt tggtgacgga ctggctggtt
     1021 cgcgtggggt atgagggctg gttcaccacc gagctcggtg gcggcgacga ggcctacctc
     1081 aaggatgtgg ctgaccgcgt cgacgcgatc ttcgctggca agtagagccc gaccggacgg
     1141 gggggctggg gaccggagag gctttccgca catcagccgc agctttgccg gtccccagtt
     1201 gccgggcgtc ggacgcctac ccggtctgga ggctttgtag cgtgcgcagc agccgttcac
     1261 gcaggtccgg cggcagttcc tcgcgctcgc cggacagcag tgcttccgcc accgccctga
     1321 gatcaggatc cagggcggac aaagaggcgt cgcttcggct catccagcgt cggagttggt
     1381 cgatcagccg ccgatctcgt cctgccatcg gtggtccccc tcgtctgatc ctcgcacaat
     1441 gcgaccgcca ggcgagcccg aagagtctgg accgcgtgtg acagtcgcca cttcaccgtc
     1501 cccagaggga tacccagagc ctgcgcgacg tccttgaggg acgcctcgcc atagcagtgc
     1561 atgacaacca cttcacggta gatggtcggc agtgccgcca cggcgttccg gacgagtcgt
     1621 cgcgagtcgg cgtcgaccac cgactgggcc agatcgaagg gcgaggcgac atcgggcgcc
     1681 tcgcactcga gctcggcgcc acgccgctga gtgcgccgca ggtgctggcg gtactggttg
     1741 agcgcgatct tgcggatcca ggtcgatagc cggcactcgc cccggaaccg tcccaacgcc
     1801 cgccagactt ggaccatggt ctcctgggtc agatcggccg ccgtctcgcg gtcctgggtc
     1861 agcatccaca ggaaatcata cgcgtcccgc atgtggcggt ccacgatctc gaccggagcc
     1921 tggcggtccc cggtgcgcag acgctcaagc agcctgtggt cgtcggggag cattcgatcc
     1981 ccctcggagt gccgtctgaa gcttctccac aatcgtagac gaccggacgc tggcgtgggt
     2041 tgggtcggag gatcagaagc cgggcaccgg gaacagccag atggatgccg aggccagtca
     2101 ggccggcatg cggataggga gttcgtgccc gatggatcgg aagttgacgc gacgcgagat
     2161 gatggctgtt ggggccctcg ggatcgcggg ggtggcggct cgtggcggcg cggtgtctga
     2221 gggggatggc cgtcgaaagg ccatgtcact cagtctgaac tcgagtgtca tccggccgac
     2281 gccgctcgaa cgcaaagtgc gtatcgcggc cgagatgggc tatgacggca tcgaactgtg
     2341 ggatgaggag ctcgaacggt accagggcga ggggcactcg ctgaccgatc tgggccaccg
     2401 aatccgggat ctggggctgt tcgtgcccaa catcattggg ttgtgggact gcatgccgcc
     2461 cgaggaatca cagcgggcca gcgcggtgga ggtggccaag cggcggatgg agcgcggcgt
     2521 cgccgtaggg gcgcggtgca tcgcggcgat ccccgcgccg gatcgggagg acatcggcat
     2581 cgagtgggcc gcggagcgct atcgcgagct gctcgaggtg ggccgggaga tggggattcg
     2641 ggtcgccgtg gagtttgtcg gcttcctgcg cggagtgcac cgcctcggc
//
LOCUS       DAOSPL010000560         2684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_38017_length_2684_cov_3.145683, whole genome shotgun sequence.
ACCESSION   DAOSPL010000560 DAOSPL010000000
VERSION     DAOSPL010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38017_length_2684_cov_3.145683"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="PLQ54_10700"
     CDS             <1..1012
                     /locus_tag="PLQ54_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015845142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOS93772.1"
                     /translation="QDGRLDIVCGGNWYEAPDWTRHPMCEVQAQGEYYDDFSDIPMDV
                     NGDGFPDIVTGGWFGETLYWRENPRGKPGPWTVHSIARIGNIETTRAWDVDGCGELEI
                     VPNTPPNDQVFFKLNRGPDGRGNGTFSMYTAMKGPTGHGHGFGDIDGDGRGEIILSGG
                     WLEPPADPLSGPWTFHDEFSLGAASNPILVHDVNGDGLADLIVGHAHGYGLHWWEQSR
                     DVDGRRTWTPHVIDMTCAQYHDMQLVDIDHDGELELVTGKRYRAHCGNDPGDNDPVGV
                     YYFKIRQGEFERHIIDWGPADSGGSGVGIYFWVEDIDGDGLLDIIAPGKEGLYLFRNL
                     GP"
     gene            complement(1046..2317)
                     /locus_tag="PLQ54_10705"
     CDS             complement(1046..2317)
                     /locus_tag="PLQ54_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93773.1"
                     /translation="MWIRGVRLVAWVVVMAPLVGCAWVPEGGGGTNVPDRALLVTLTL
                     ADRVDANYYYYLAIDTDDDRTDGPLPLYEGPFWGNGWGTGSMTYFVSYHAGAYGVYRP
                     AILADLVPRSITGEPVETGIAAVSGSPTAPVAGIHKLTVDRINYGAVAIDTAGTVQSA
                     ANASFQGAGTLTIGTDAEGRVAAGSIVFTPAETGGRPLTSAEQSAVADLNRGGALLAP
                     NSLSALGLALTLAPTPAAGVQTLTIAPTTGAVTDRFEAYFDTGDYMASGVLYANQANS
                     EGPTPILPGVRFTTGDLVQGGVSNVITQFDVTANYLGLPFAGTVPRGTGLASRQLAFE
                     IDLAQIGDPQDRIDLNFITTDELIFDPEIAGPKRYDGLGNTGNQFVTLNLNTNYTYAN
                     DDATYPEYSGDANPAAIDIVDWRIEVILANR"
     gene            2460..2681
                     /locus_tag="PLQ54_10710"
     CDS             2460..2681
                     /locus_tag="PLQ54_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93774.1"
                     /translation="MRNAITPLLLVATCPCLAQVPVVDATTIRGKVMCGYQGWFRCPG
                     DAANMGWVHWSRDSARIAPDTLTFEMCPT"
BASE COUNT          442 a          878 c          912 g          452 t
ORIGIN      
        1 ccaggatggc cgcctggaca tcgtgtgcgg cgggaactgg tacgaggccc ccgactggac
       61 gcggcatccc atgtgcgagg tccaggctca gggcgagtac tacgacgact tctccgacat
      121 ccccatggat gtcaacggcg atgggttccc cgacatcgtc accggcggct ggttcggcga
      181 gaccctctac tggcgcgaga acccccgggg caagcccggc ccctggaccg tgcactcaat
      241 cgcccggatc ggcaacatcg agaccacccg cgcgtgggac gtggatggct gcggcgaact
      301 cgagatcgtc ccgaacacgc cgcccaacga ccaggtcttc ttcaagctca acaggggccc
      361 ggatggcagg ggtaatggga cgttcagcat gtacaccgcg atgaagggcc ccacgggtca
      421 cggtcatggt tttggggaca tcgacggcga tggccggggt gagatcatcc tctcgggcgg
      481 ctggctcgag ccgccggccg atccgctgtc gggtccgtgg acctttcacg acgagttcag
      541 tctcggcgcc gccagcaacc cgattctggt tcacgatgtg aacggcgacg gcctcgctga
      601 cctgatcgtc gggcacgccc acggctatgg cctccactgg tgggagcagt cgagagacgt
      661 cgacggccgc aggacctgga ctccgcatgt catcgacatg acctgcgcgc agtatcacga
      721 catgcagctg gtcgacatcg accacgatgg cgagctcgag cttgtgaccg gcaagcgcta
      781 tcgggcccac tgcggcaatg acccgggcga caacgatccc gtgggcgtct actacttcaa
      841 gattcgtcag ggcgagttcg agcgccacat catcgactgg ggtccggcag acagtggcgg
      901 gagcggcgtc ggcatctact tctgggtaga ggatatcgac ggggatggcc tgctcgacat
      961 catcgcgcct gggaaagagg gcctctacct cttccgaaac ctggggccgt aggccggccg
     1021 ggccctctgc cctgtgccgt ccgggtcacc ggtttgccag tatgacttcg attcgccagt
     1081 cgacgatatc gatggccgcg gggttggcgt cccccgagta ctccggatag gttgcgtcat
     1141 cattggcgta ggtgtagttg gtgttcaggt tcagcgttac gaactggttg cccgtgttgc
     1201 cgaggccatc gtagcgcttg gggccggcga tctctggatc gaagatcagc tcatcggtgg
     1261 tgatgaagtt gaggtcgatg cggtcctgtg ggtcgccgat ctgcgcgagg tcgatctcga
     1321 aggccaactg ccggctggca agcccggttc cgcgcgggac cgtgcccgcg aagggcagtc
     1381 ccaagtagtt ggcggtgacg tcgaactggg tgatcacgtt ggacacgccc ccctgcacca
     1441 ggtcccctgt cgtgaaccgg acgccgggta ggatgggcgt tggcccctcg gagttggctt
     1501 ggttggcgta cagcacgccg ctcgccatat agtcgcccgt gtcgaagtac gcctcaaacc
     1561 gatcggtcac agcgcccgtc gtgggcgcga tggtcagcgt ctggacgccg gcggccggcg
     1621 tgggcgcgag cgtcaacgcc agtcccaggg cgctcaaaga gttgggggcc aggagggcgc
     1681 cacctcggtt caggtcggcg acggcgctct gctccgccga tgtcagcggg cgcccgccgg
     1741 tctccgccgg ggtgaacacg atgctgccgg cagcgacccg gccctccgcg tcggtgccaa
     1801 tcgtcagcgt acccgccccc tggaaggaag cgttggcggc ggactggacg gtgccggcgg
     1861 tatcaatggc gacggcccca tagttgatgc ggtccacggt cagcttgtgg atgcccgcca
     1921 cgggtgcggt tgggctgccc gagacggcag caatgcccgt ctcgaccggc tcccccgtga
     1981 ttgagcgcgg cacgagatca gccaggatgg cagggcggta cacgccgtac gctcccgcgt
     2041 ggtacgagac gaagtaggtc atcgagcccg ttccccagcc gttgccccag aagggcccct
     2101 cgtagagcgg caggggcccg tcggtgcggt cgtcatcggt gtcgatggcc aagtagtagt
     2161 agtagttcgc gtcgacccga tcggccaagg tgagcgtgac cagcagggca cggtccggca
     2221 cgttcgtgcc gccgccgccc tcggggaccc aggcgcaccc cacgagaggt gccataacga
     2281 ccacccacgc cacaagccga acgccgcgga tccacacact ggccgcctcc tgctctccca
     2341 ttcgcttact atacaggacg gtccaccgcc cggcggtact cagcgtggtc cctccgcagg
     2401 ctggccgcgt ccgcccagcg aacccgtgtt gccagacgca ccgacgggag tgcccatcgt
     2461 tgaggaacgc catcaccccg ctactgctcg tcgcaacgtg cccgtgtctc gcccaagtcc
     2521 ctgtggtcga tgccacgacg atccgcggca aggtcatgtg cggctaccag ggctggtttc
     2581 ggtgcccggg ggacgcggcg aacatgggct gggtccactg gagccgcgac tcggcgcgga
     2641 tcgcgcccga cacgctcacg ttcgagatgt gcccgacatg agcg
//
LOCUS       DAOSPL010000561         2683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_38040_length_2683_cov_3.319254, whole genome shotgun sequence.
ACCESSION   DAOSPL010000561 DAOSPL010000000
VERSION     DAOSPL010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38040_length_2683_cov_3.319254"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1067)
                     /locus_tag="PLQ54_10715"
     CDS             complement(21..1067)
                     /locus_tag="PLQ54_10715"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS93775.1"
                     /translation="MADPRVERNRSHLARLFAGSFPGHAVVMSAESACPPLPGDVTCP
                     DRPVGLWVDRALRQYEAELRVLAELDDDSVPVARITTGTQLFAAAFGCRVHLFVDSPP
                     CALPLVETAHQADALVEPEPDAGPLARVYEAAARLRERLGPDVPIRVPDIQSPFDIAA
                     LIWRKESLYTAVVDDPEAVKRLVSKCTRLLRRFLCRFMAEVGECSLAHCPDVWAPPEL
                     GCWLSEDEAGAMSPAMFEEFCLPPLIELSEAFGGLFVHCCATADHQYPNFRRIPNLRG
                     LNRVFQAPGPRPAIEAFTPGTVLMVAWTGIEACREMLDMALRETRFLFNMPAEPLGQA
                     KATYERMREWCPRL"
     gene            complement(1144..1326)
                     /locus_tag="PLQ54_10720"
     CDS             complement(1144..1326)
                     /locus_tag="PLQ54_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93776.1"
                     /translation="MSATLERVASAEALLAEGHQAEAVGALRDALRASVSYAEQMVIL
                     ARLRALAERAQPTVSR"
     gene            1909..>2683
                     /locus_tag="PLQ54_10725"
     CDS             1909..>2683
                     /locus_tag="PLQ54_10725"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOS93777.1"
                     /translation="MRVADYLGPETPAGLDPPETPEWTHMVRLAADHGLAPLLHRRLR
                     DLGQLECLPGPEAEALAARHADAVGRYGEIGLWALPELVALRDAFDAIVLKGAALAHR
                     IYPSPHLRLFRDLDVLIRPEDRGECRRMLATRGWRLIGRGRLEDQWTKGPAGPGRMSL
                     DVHTHIGDPALYPIPAWATVWSSRAGSRLWGTPFPVLPLEMSALQMALQFATNLWYGA
                     VQLSQLVDLRYLIADPQFDRARAEEESERCGLLGVLAVST"
BASE COUNT          462 a          926 c          912 g          383 t
ORIGIN      
        1 cgtaggcgac aggaggctgc tcaaaggcga gggcaccact cgcgcatccg ctcgtaggtg
       61 gccttggcct gacccagcgg ctcagccggc atgttgaaga ggaagcgtgt ctcgcgtaga
      121 gccatgtcga gcatctcgcg acaggcctcg atgccggtcc atgccaccat gagcaccgtg
      181 ccgggcgtga aggcctcgat ggcggggcgt gggccgggag cctggaacac gcggttgagg
      241 ccccgcaggt tcggaatccg ccggaagttg gggtactgat gatccgccgt cgcgcagcaa
      301 tgaacgaaca gcccgccgaa ggcctcggac aactcgatca acggaggcaa acagaactcc
      361 tcgaacatgg ccggcgacat ggcgcccgcc tcatcctccg acagccagca accgagctcg
      421 ggcggtgccc agacatcggg acagtgagct agagagcact cgccgacctc cgccatgaag
      481 cggcacagga atcgccgaag caggcgcgtg cacttgctga ccagccgctt cacggcttcg
      541 gggtcatcga ctaccgcggt gtacagggac tccttccgcc agatcagcgc ggcgatgtcg
      601 aaggggctct ggatgtcagg cacccggatg gggacgtccg ggccaaggcg ctcgcggagt
      661 cgcgcagcag cttcgtaaac ccgggccagg ggaccggcgt ccggctcggg ctccacaagg
      721 gcatcggcct ggtgggccgt ctcgaccagc gggagggcac agggcggcga atccacgaag
      781 agatgtacgc gacacccgaa cgcagcggca aagagctggg tgccggtggt gatgcgcgcc
      841 acgggtaccg agtcatcgtc cagttcggcc agcacgcgca gctcggcctc atactgacgc
      901 aaagctcggt ccacccagag cccgactggc cgatccggac aggtgacatc gccgggcagc
      961 ggcggacatg ccgattccgc gctcatcacg accgcgtgac cggggaaaga ccccgcaaac
     1021 agccgagcca aatgcgagcg attgcgctcg acccgcggat ccgccatgcc gcatccccca
     1081 tcggtgggtc acgcggcaac gatcgagcgc ccatcggcgc ccaccacgcc cgtgcccgca
     1141 agctcagcgc gatacggtgg gctgggcccg ctcggccaga gcgcgcaggc gggccagtat
     1201 caccatctgc tcagcatacg acacggacgc ccgcagagca tcccgcaggg caccgacagc
     1261 ctcggcctga tggccctcgg cgagcaacgc ctcggccgag gccacacgct ccagagtcgc
     1321 gctcatggcc ggcgactcat cggccgcagc ggtgtcagaa gcggcctcac gggcaggcgc
     1381 agccccgcac tgcgcgaacg ggatacgtag acaacgtgat cgaaccaccc caaccccacc
     1441 ccctgcacaa ccacgcggca ggacatgcct gccgcttctc catttccgtt gttccccctt
     1501 gacgtagccg aaacagcact tcggcgtaac attagtgtaa cagtaccggg ctcccgcgtc
     1561 cacggccagg agtctcctac ccattggacg ttcgagcgag ccagaaggga gcatcgcccg
     1621 cccacatccc ggttgcgtgc gcgcacgggg agagcgtgtg gcctgagggg ccgggctggg
     1681 cgttcggtgt ggggcgcgcg gaagcgggag accatatgcc aggttcggct acccaagcac
     1741 cacgagtggt ctgcgacgat ctgcttgtcg gcgcgggagt tgcacgagcc gcctcagacc
     1801 cccgggcaca tcgccttcaa gggacgcggc agcccggggg gccgcggcgg ggaaggtcgg
     1861 gatggccggg cagacgcatt ctgccccatg gaccgatgga tgacctgggt gcgagtcgct
     1921 gactacctcg gacccgagac gcccgccgga ctggacccgc ctgagacgcc cgagtggacc
     1981 cacatggttc ggctcgcggc tgaccacggc ttggcgccgc tgctccatcg tcggctgcgg
     2041 gatctcgggc aactggagtg cctcccgggt ccggaggcgg aggcgctggc agctcggcat
     2101 gcggatgcgg tgggccgcta cggagagatc ggcctctggg cgctgcccga gttggtggcc
     2161 ctccgcgacg cgttcgatgc catcgtgctc aagggcgccg ccctggctca tcggatctac
     2221 ccctctcccc acctccggct gttccgtgac ctcgatgtcc tgatccggcc cgaggaccgc
     2281 ggcgagtgcc gtcgcatgct ggcaacccgc ggatggcggc tcatcggccg gggacggctc
     2341 gaggaccaat ggacgaaagg accggccggg cccggcagga tgtcgctgga cgtgcacacg
     2401 cacatcggcg acccggcgct gtaccccatc cccgcatggg cgaccgtctg gtcctcacgg
     2461 gcggggtctc ggctctgggg caccccgttt cccgtgctgc cgctggagat gtcggcactc
     2521 cagatggcgc tgcagttcgc cacgaatctg tggtacggcg cggtgcagct ctcgcagctt
     2581 gtggacctcc gctacctcat cgccgatccg cagttcgatc gggcccgggc cgaggaggaa
     2641 tccgagcgat gcggccttct cggtgttctc gccgtgagca ctc
//
LOCUS       DAOSPL010000562         2683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_38046_length_2683_cov_2.669711, whole genome shotgun sequence.
ACCESSION   DAOSPL010000562 DAOSPL010000000
VERSION     DAOSPL010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38046_length_2683_cov_2.669711"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PLQ54_10730"
     CDS             complement(<1..826)
                     /locus_tag="PLQ54_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HOS93778.1"
                     /translation="MNRIKVDSERQTASVDPRIYGNFVEHLGRCVYGGIYDPGSPRSD
                     ADGMRLDVLEATQRLAPTQVRYPGGNFVSGYHWLDGVGPRDARPRRMELAWHTVETNQ
                     FGTNEFVQWCRRVPTEPVFCVNLGTGTIEEARDWLEYCNGTGDTHFAQLRRSHGFSEP
                     HGIRVWDLGNEIDGDWQIGAKTAEEYGRAAVECAKLMKWTDPSIELVACGDSFARDDW
                     NRTVLRHLNGWANYLSVHMYIGRQNRDVQAYLVESRAQIERRISRVAAQITAEELRQ"
     gene            complement(871..2619)
                     /locus_tag="PLQ54_10735"
     CDS             complement(871..2619)
                     /locus_tag="PLQ54_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93779.1"
                     /translation="MAFATPSGEVTRWVAAEDAPRWRDWAGARDAGAAADITRHQLRR
                     RAFVTADELHQRYGLGLEDARTALDEAVKAGAAIRFGAPGSPELDRYADPRHADAAYR
                     ITLTMARSEADPASPEEFCRFLLRWQCVSPRTRRSGLEGLADVLVQLQGRPLPFSVWE
                     AAVLPARVRDYSPSWLDELCSGGLVAWRGLPGPGPSDGRIAFYRRENLARWAPAGVDA
                     TEGDVPERVRGALRRLGPSFLADIAAEAGVSAHETLGALWDMMWHGEVAQDDFRAVRR
                     GRPDEAEARQIGARLAVGGARGGFLGAAMPRWSLLPPGQLDAEAFTSDRTLAETVGWL
                     LLARNGIMARGLLEAEDVPLPWGALYDVYQRLEMQGKVARGYFVAGLEGAQFALPEAA
                     DALRGVQTESDREAEGPVLLNTCDPANPYGAGAPWAVTDASGAEVPCHRNRQNSLVVD
                     QGRPALLIEWGAERVTALVPSDETSLRRLLLPVREALESPSAARSARRLRVVRFGDAP
                     VLHSPIEPPLRDAGFQSDGQGLVYVGLPRPAKGSDAEGWPTPRPAPRGTVPKLPYPRP
                     PGRRRGKGRRQGQGPP"
BASE COUNT          438 a          953 c          898 g          394 t
ORIGIN      
        1 tctgccgcag ctcctcggcc gtgatctgtg ccgcgacacg gctgatccgg cgctcgatct
       61 gggcgcgcga ttccaccaga taggcttgca catcccgatt ctgcctcccg atgtacatgt
      121 gtaccgacag gtagttggcc cacccattca ggtgacgcaa cacggtccgg ttccagtcgt
      181 cacgcgcgaa tgagtctccg caagcaacca gctcgatcga agggtccgtc cacttcatca
      241 gtttggcgca ctcgaccgcg gctcgaccat actcctcggc ggtcttggcg ccgatctgcc
      301 agtcgccgtc gatctcattc cccaggtccc acacgcggat cccgtgaggt tcgctgaaac
      361 catggctgcg ccggagctgg gcgaagtgag tgtcgccggt gccattgcag tactcgagcc
      421 agtctcgggc ctcctcgatc gtgcccgtac ccaggttcac gcagaagacg ggctctgttg
      481 gcacccgccg gcaccactgg acgaactcgt tcgtgccgaa ctggttggtc tcgaccgtgt
      541 gccaggccag ctccattcgg cgcggccgcg catcgcgcgg ccccacgcca tcgagccagt
      601 gatagccgga gacgaagttg ccgcccgggt accggacttg cgtgggggcg agccgctggg
      661 ttgcctcgag cacatcgagg cgcataccgt cggcatcgga tcgagggctc ccggggtcgt
      721 agatgccgcc gtagacgcag cgtcccaggt gctcgacgaa gttgccgtaa atcctgggat
      781 ccacggaggc ggtctgacgc tccgagtcca ctttgatgcg gttcatgggc cccgacctcc
      841 aagccgaacg gcgtcccagt tcgatgggca ctagggaggg ccctgcccct gacggcggcc
      901 tttgccacga cgacggccgg ggggcctggg atagggcagc ttgggcaccg tgccgcgcgg
      961 cgcgggacgc ggtgtgggcc agccctcggc gtcagaaccc ttcgccgggc gcggcagccc
     1021 aacgtagaca agcccttggc cgtcgctctg gaagcccgca tcgcgcagcg gtggctcgat
     1081 agggctgtgg aggaccgggg catcgccaaa gcggacgact cggagcctcc tggccgaacg
     1141 cgcagcagaa ggtgactcga gtgcctcgcg gactggcagg agcagacgcc gcagactggt
     1201 ctcgtcagac gggacaaggg cggtcacccg ttcggcgccc cactcgatca gcagggccgg
     1261 gcgaccctga tccacgacca gtgagttctg gcgattgcgg tggcagggga cctcagcgcc
     1321 cgaagcatcc gtgactgccc atggcgcacc cgcaccgtac gggtttgccg ggtcgcaggt
     1381 gttgaggagc acgggccctt cggcctccct gtctgactcg gtttgtactc ctcggagggc
     1441 atcggcagcc tcaggcaggg caaactgcgc cccctcgagc ccggcgacga aatagccgcg
     1501 cgccaccttg ccctgcatct cgaggcgctg gtagacatca tagagggcac cccagggcag
     1561 cggcacatcc tcagcctcga gcagacctcg ggccatgatg ccgttccggg ccaacaggag
     1621 ccagcccacc gtctcggcga gtgtgcggtc gctggtgaat gcctcggcat cgagttgccc
     1681 cggcgggaga agcgaccacc ggggcatggc ggcgcccagg aacccgcctc gggcacctcc
     1741 gacggccaga cgagcgccga tctgccgcgc ctcggcctca tcgggccgac cccggcgcac
     1801 ggcgcggaag tcgtcctgag ccacctcgcc gtgccacatc atgtcccaca acgccccaag
     1861 tgtctcgtgc gccgacacac ccgcctccgc cgcaatgtcg gcgaggaaag acggcccaag
     1921 ccgccgcaga gctccgcgga ccctctcggg cacatcgccc tctgtggcat ccactccggc
     1981 gggtgcccag cgcgcgaggt tctcgcggcg gtagaacgcg atgcgcccgt ccgagggccc
     2041 ggggcccggc aagccgcgcc aggcgacaag gccgccggag cacagctcat caagccatga
     2101 cgggctgtag tcgcggaccc gggcaggaag cacggccgcc tcccacacag agaacggaag
     2161 cggccggccc tgcagttgga cgaggacgtc ggccaacccc tccagaccac tgcggcgcgt
     2221 ccgcggcgac acacactgcc agcggagaag gaaccggcag aactcctcgg gcgaggccgg
     2281 atccgcttcg gaccgcgcca tggtgagcgt gatgcggtag gcggcgtcgg cgtgccgcgg
     2341 gtccgcgtac cggtcgagct caggtgagcc gggagcgccg aagcggatgg ccgcacccgc
     2401 cttgactgcc tcatcaaggg ccgtccgcgc gtcttcgagc ccgaggccgt accgctggtg
     2461 cagttcatcg gccgtgacga acgctcgacg acgcagttga tggcgagtga tatcggcggc
     2521 ggcgccggcg tcacgcgcgc ctgcccagtc ccgccacctg ggagcatcct ctgcggcgac
     2581 ccagcgagtg acctcgcctg agggagtggc gaaggccacg ggcccacgct actccgccgc
     2641 gatgcgctcc aacccgccga ttcacatccg cgatctgctc gac
//
LOCUS       DAOSPL010000563         2677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_38149_length_2677_cov_3.895881, whole genome shotgun sequence.
ACCESSION   DAOSPL010000563 DAOSPL010000000
VERSION     DAOSPL010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38149_length_2677_cov_3.895881"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1207)
                     /locus_tag="PLQ54_10740"
     CDS             complement(29..1207)
                     /locus_tag="PLQ54_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOS93780.1"
                     /translation="MKTQLEQYTVAEVTKGFVYNELEGKGLFGLDGTLVIQPEFQRSY
                     LYGDGTKDVAVIDSLLKGYPLGLIYFVDTGDPSGPHLEVLDGQQRITSVGRFVTGKFA
                     IMRDGREQTFGSLPKEDQDRITEAKLLVYVCTGTEAEIKEWFQTINIVGIPLNAQELR
                     NAIYSGPFVTAAKAEFSNSQNALQQKWSAYVKGDPKRQQVLEVALDWVASSKDQSIDG
                     YMAAHRTDAGIGELKTYFTTVIDWVSGVFTAPPDAAMRGLDWGALYEEHKGTAYQPDR
                     IDERLQDLLADPAVNDHKGIYPYLLGGEDDPRLLNVRLFDAPTKKAAYKRQTDKANAE
                     GTSNCPLCAVGSNANKARIYKLKEMEADHVTAWSTGGDTSLDNCEMLCVTHNRSKGNR
                     "
     gene            complement(1211..2386)
                     /locus_tag="PLQ54_10745"
     CDS             complement(1211..2386)
                     /locus_tag="PLQ54_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific methyltransferase EcoRI family
                     protein"
                     /protein_id="HOS93781.1"
                     /translation="MANVDLGAAKAAKKDAFYTSWSDIEREMNAYLEYDPDVFRGKTI
                     LLPCDDPEWSNFTKFFALHFADFGLKKLISTSYAHDDNAPSGYEPPTLFDEPNTMGEQ
                     RGRVFTLLPEDLSGDGRVDIDDLRWEYLEGDGDFRSAEVTALRDEADMVITNGPFSLF
                     RAFIKWLVEGGVQFSVIGNVNAVSTKEIFPLIRDNKLWMGPTITSGDREFRVPASYPL
                     EAAGFRVDEDGNKYIRVKGVRWFTNIDHGRRHAPLRLMTEADNIKFSKHKEVRGLGYR
                     RYANYDGIEVPFTDAIPSDHAGLMGVPITFLDKYNPEQFEILGISKAWDQDSGLRTRL
                     FPPQTQVAKSGARSQVGKLNDGTAIQVATRPSTTHYEVEGEMFIQTYPRVLIRAKKG"
BASE COUNT          439 a          808 c          861 g          569 t
ORIGIN      
        1 cgaccgatgc cttcgaggag agcggcgtct agcggttgcc cttgctgcgg ttgtgggtga
       61 cgcagagcat ttcgcagttg tccagggagg tgtcgccgcc ggttgaccag gcggtcacgt
      121 ggtcggcttc catctccttg agcttgtaga tgcgcgcctt gttcgcattg ctgcccacgg
      181 cgcacagggg gcagttcgat gtgccctcgg cgttggcctt gtcggtctgg cgcttgtagg
      241 cggccttctt cgtcggcgcg tcgaacaggc gcacgttgag cagccggggg tcgtcctcgc
      301 cgccgaggag gtaggggtag atgcccttgt ggtcgttgac ggctgggtcg gccagcaggt
      361 cctgaagtcg ctcgtcgatc cggtcgggct ggtaggcggt gcccttgtgc tcctcataga
      421 gggctcccca gtccagcccg cgcatggccg cgtccggagg ggcggtgaac actccggaga
      481 cccagtcgat cacggtggtg aagtaggtct tcagctcgcc gatcccggcg tcggtgcggt
      541 gggcggccat gtagccgtcg atgctctggt ccttcgagct ggcgacccag tcgagcgcca
      601 cttcgagcac ctgctggcgc tttgggtcgc ccttgacgta agccgaccac ttctgctgga
      661 gggcgttctg actgttggag aactcggcct tggccgcggt gacgaacggg ccggagtaga
      721 tcgcgttgcg cagctcctgg gcattgagcg ggatgccgac gatgttgatc gtctggaacc
      781 actccttgat ctcggcctcg gtgccggtgc agacgtagac cagcagcttc gcttctgtga
      841 tccggtcctg gtcctccttc ggcagtgagc cgaaggtctg ctcgcgcccg tcgcgcatga
      901 tggcgaactt gccggtgacg aagcggccaa ccgaggtgat ccgctgctgg ccgtcgagga
      961 cctcgaggtg ggggcccgag gggtccccgg tgtcgacgaa gtagataagc cccagcgggt
     1021 agcccttcag caaggagtcg atgacggcca cgtccttcgt gccgtcgccg tacaggtagc
     1081 tgcgctggaa ctcgggctgg atcaccaggg tgccgtcgag cccgaacaga cccttgcctt
     1141 cgagctcgtt gtagacaaac cccttggtga cctcggccac cgtgtactgc tccagctggg
     1201 tcttcatggg tcagcccttc ttcgctcgta tgagaacccg agggtaagtc tgaatgaaca
     1261 tctcgccctc gacctcgtag tgggttgtcg atggcctcgt tgcgacctgg atcgcggtgc
     1321 catcgttgag cttgccaacc tgagatcgcg cccctgactt ggctacctgc gtctggggtg
     1381 ggaataagcg tgtccgcaga cccgagtctt ggtcccatgc cttcgagatg cccaggatct
     1441 cgaactgctc cggattgtac ttatccaaga acgtgatggg cacgcccatg aggccggcat
     1501 ggtcgcttgg gatagcgtcc gtgaacggca cttctatgcc gtcgtagttc gcgtaccgcc
     1561 tgtaaccaag gccacgcact tccttgtgct tgctgaactt gatgttgtcg gcctcagtca
     1621 tgagacgcaa cggcgcatgc cgtcgaccat ggtcgatgtt cgtgaaccac cgaacgcctt
     1681 tcacccggat gtacttgttc ccatcctcgt cgacacggaa tcccgccgcc tccagcgggt
     1741 agctggcggg caccctgaac tcgcggtcac cgctagtgat cgtcggaccc atccagagct
     1801 tgttatcccg aatgaggggg aagatctcct ttgtcgaaac ggcgttgaca ttcccaatga
     1861 cagagaactg aacgccaccc tccaccaacc acttgataaa ggcgcggaac aggctgaagg
     1921 gtccgttggt gatgaccatg tctgcttcgt cgcgcagagc ggtcacctcc gctgagcgga
     1981 agtcaccgtc gccttcgagg tactcccagc gaaggtcgtc gatgtcgacc cgtccgtcgc
     2041 cggagaggtc ttctggcagc agggtgaaca cccggccgcg ttgctcgccc atggtattgg
     2101 gctcatcgaa cagggtgggc ggctcgtagc cggacggtgc gttgtcgtcg tgtgcatagc
     2161 tggtcgagat gagcttcttc agaccgaagt ccgcgaagtg cagggcgaag aacttggtga
     2221 agttgctcca ctccgggtcg tcgcagggca gcaggatcgt cttgcctcgg aacacatcgg
     2281 ggtcgtactc caggtacgcg ttcatctccc gctcgatgtc ggaccacgag gtgtagaagg
     2341 cgtccttctt agcggccttg gcggctccca ggtctacgtt ggccatgtcc ctcgatctcc
     2401 tcgtccctca acagacaaga gtatccgggg gtgcggacac tgcgcagcac cggcgctatc
     2461 ccctgtagcc atgcgggcag cccccgcagg agagtccggg acgcccgtgg gcctcctcgg
     2521 gtgatgtatg aagtcggaat cctgtgtgtt tgcggtgact ttagcggtca ctttgaccca
     2581 gaaaaagaca atgacctggt cagaacatcg ttctgaccag gtcattcgtc gggctgacag
     2641 gatttgaacc tgcgacccct tgacccccag tcaagtg
//
LOCUS       DAOSPL010000564         2676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_38180_length_2676_cov_2.185807, whole genome shotgun sequence.
ACCESSION   DAOSPL010000564 DAOSPL010000000
VERSION     DAOSPL010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38180_length_2676_cov_2.185807"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PLQ54_10750"
     CDS             complement(<1..799)
                     /locus_tag="PLQ54_10750"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein"
                     /protein_id="HOS93782.1"
                     /translation="MADSPKCYLGIVLHGHLPYVMAHGRWPHGMDWICEAATECYIPL
                     LRMAENLRRSGRHSALTMGITPVLAEQLRDGDFPAELDAYCEERVRRAVEAEAYLEST
                     GDHRVAGMARWWREYYDGVKRSFHEEYGADLVGAFARCQELGQMEIITCAATHGYLPL
                     LGRDTSVQAQVRTGVEVYEKHFGRKPRGFWLPECAYRPRYEWAPPAECGPARPPVLRK
                     GVAEFLHEAGIRYFIVDTHLLRGGKALGVYRHRYKALEQLWAQFESQC"
     gene            1017..2153
                     /locus_tag="PLQ54_10755"
     CDS             1017..2153
                     /locus_tag="PLQ54_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS93783.1"
                     /translation="MANELERFMDCMEYRPSDRRPNHELGVWPQTIERWRAEAADAIE
                     GFTWNWFWAEDALGFDRREYLRIHYGFLPPYEYEVLEETPEYVVARNGNGIVTRALKE
                     GTVGGGRMCMDQYLEFPVSTPDDFPAVKARLVAGAPERYPESLEAQIQAAANRTCPLV
                     LGENCAANGFYWRAREWMGTEALSLAWYDYPALMHEMMEFYADFIIETSRPVLERCQP
                     DYFCLNEDLAMKGGPLLSPSTFRTFILPHLSRMVETFKGLGVRYFVIDTDGDPRPLLS
                     LFLDAGVDAIWPLERASDMDPAELRRTFGRSLRLWGGVDKRELAKGPDAIRRHLVEFI
                     PLIEEGGFIPTVDHTVPPDVSWDNFRYYMDLKHELLAGQFGNLR"
     gene            complement(2491..>2676)
                     /locus_tag="PLQ54_10760"
     CDS             complement(2491..>2676)
                     /locus_tag="PLQ54_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93784.1"
                     /translation="DIIEDVIPLFHVPVSVRVAGAVTAVTLEPQAEAVPFAQAGDRVE
                     FIVPKVDGHQMVAIACE"
BASE COUNT          458 a          837 c          902 g          479 t
ORIGIN      
        1 cgcactggct ctcgaactgg gcccagagct gctccagggc cttgtatcga tgtcggtaga
       61 cccccagggc ctttcctccg cgaagcagat gggtatccac gatgaagtac cggatgcccg
      121 cctcgtgaag gaactcggcg acccccttgc gcagcacggg tgggcgtgcc ggtccgcact
      181 cggcgggtgg cgcccactcg taccggggcc ggtaggcaca ctccggcagc cagaaacccc
      241 ggggcttccg gccgaaatgc ttctcgtaca cctcgacgcc ggtccggacc tgggcctgaa
      301 cgctcgtgtc gcgcccaagc aggggcaggt agccgtgggt ggccgcacag gtgatgatct
      361 ccatctgccc gagttcctgg caccgggcga aggccccgac cagatcggca ccgtactcct
      421 catggaacga ccgcttgacg ccatcgtagt actcgcgcca ccaccgggcc atcccagcca
      481 cccggtggtc gcccgtggac tccaggtagg cctcggcctc gaccgcgcgc cggacgcgct
      541 cctcacagta ggcgtcgagc tcggccggga aatcaccgtc gcgtagctgc tcggccagca
      601 cgggagtgat ccccatggtg agcgccgagt gacgcccgga acggcgcagg ttctcggcca
      661 tacgcagcaa tgggatgtag cactcggtgg ccgcctcgca gatccagtcc atgccgtgag
      721 gccatcggcc gtgggccatc acatagggga gatgcccgtg cagaacgata ccaaggtaac
      781 acttgggcga gtcagccatg agccctcccg catgcccgca cgacggatca gcggcgcttc
      841 agcggaaccc tcggacggcc agtgataccg cgttggcggg ggccgcggcg cgcccgggaa
      901 cacgaggcgc attctatcgc gggcttggag gccggtcaaa cgcacaccgg gccggctcgg
      961 tgaaggaaac cgtgaggcct tgccgcgaaa ccgtttcgct gaagggatgg atcccgatgg
     1021 ccaatgagct tgagcgcttc atggactgca tggagtaccg ccccagcgat cggcgcccca
     1081 accacgagtt gggggtgtgg ccgcagacca tcgagcggtg gcgcgcagag gcggccgatg
     1141 cgatcgaggg tttcacgtgg aactggttct gggccgagga tgccctgggg ttcgatcggc
     1201 gcgagtacct gcgtatccac tacggtttcc tgccccccta cgagtatgag gtgctggagg
     1261 agacaccgga gtacgtggtc gctcgaaacg gcaacggcat cgtcacccga gccctcaagg
     1321 agggcactgt gggtggcgga cgcatgtgca tggatcagta tctggagttc cccgtgagca
     1381 caccggatga cttcccggcc gtgaaggctc gcctcgtggc tggggcgccg gagcggtacc
     1441 ctgagagcct cgaggcacag atacaggctg ccgccaaccg gacctgtccg ctcgttctcg
     1501 gcgagaactg cgccgccaat gggttctact ggcgtgcgcg cgagtggatg ggaaccgagg
     1561 cactctctct cgcctggtac gactacccgg ccctgatgca cgagatgatg gagttctacg
     1621 cagacttcat catcgagacc agtcggccgg tgctggagcg ctgtcagccg gactacttct
     1681 gcctcaacga ggacctggcg atgaagggtg gcccgctgct gagtccgagc accttccgca
     1741 cgttcatcct cccgcatctc agtcgaatgg tcgagacctt caagggtctg ggcgttcggt
     1801 acttcgtcat cgacaccgat ggcgacccac gtccgctgct ctctctgttc ctggatgccg
     1861 gcgtggacgc gatctggccg ctggagcgcg cctccgatat ggacccggcg gagctgcgac
     1921 ggacctttgg gcgctcgctg cgcttgtggg gcggggtcga caagcgcgag ctggccaaag
     1981 ggccggacgc catccgcagg cacctggttg agttcatccc gctgatcgaa gagggggggt
     2041 tcattccgac ggtggaccac acggtgccgc cggatgtgtc gtgggacaac ttccgttact
     2101 acatggatct caagcacgag ctgctggcgg gccagttcgg caatctgcgg tagcgtgtgc
     2161 cgggcgcacc agctcgcata gactattata gacggttttt cctggcatgt caagaacttt
     2221 tgtttgcgaa ttcagggccg cttcattggc gagggcgggc cggatggggc tcttcccatt
     2281 cggtgcttct cagtggttcg gaggatatcc tcggagaggc cgcacagggg gaatcggccg
     2341 ccggctggtg ggaccttacc cggtccgctg tagctggcgc ctgaggacgc tcacgggcga
     2401 tgggaatgtt tggtataccc cgatggtagc aagtggccag cttatatgat gagcatatca
     2461 gctggccact cgggtttggg tttcggggat ctactcacag gcgatggcga ccatctggtg
     2521 cccgtccacc ttgggcacga tgaactcgac gcggtcgccg gcctgcgcga atgggactgc
     2581 ctctgcttga ggttcgagtg ttaccgccgt gactgcccca gcgacccgca ccgagacggg
     2641 cacgtggaag agggggatga cgtcctcgat gatgtc
//
LOCUS       DAOSPL010000565         2672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_38245_length_2672_cov_2.444402, whole genome shotgun sequence.
ACCESSION   DAOSPL010000565 DAOSPL010000000
VERSION     DAOSPL010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38245_length_2672_cov_2.444402"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PLQ54_10765"
     CDS             <1..281
                     /locus_tag="PLQ54_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93785.1"
                     /translation="AISVHCGETPTVESWCRIRATGGRPWLWIEDGAERYDIARAYDS
                     HGGRAIIYFQSAFWRNAFLGDESAVPTHAQREAWRLAWLMLQTMRRAG"
     gene            complement(278..1831)
                     /locus_tag="PLQ54_10770"
     CDS             complement(278..1831)
                     /locus_tag="PLQ54_10770"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HOS93786.1"
                     /translation="MLSLLLAAVALAAGEYPVPPPEPVHGDVMLGCYYFPGHFNPLRW
                     RPMVEYGEPVPVLGYYRDGEPEVSDWHIRFALEHGIRFFVFDWYYDHTTRTVSFHNTA
                     LDDGFLRSRYGPLMRFGLMWCNESPGQPDYTRAHMVGLAEVLVERYLARENYLRIDGR
                     PMLWISRPLHLIDGFGADGLRDVIGEMNGVLAQAGIGPLFLVANATERLDEVARAGFD
                     AVSAYNYPTAGMGPGERRAPYATMVTGIRAIWRDVSERSSLPYIPPISPGWDSRPWYG
                     EDTLVRTDPSPELFERMCRDSLEFVDPKLNMVIAECWNEFGEGSYVEPTLRYGFGSLD
                     AMRRVFCPTAGPHRDLTPQALQVPVPVFDEIPSLTEADVAAQGGNRVYNPGAERRWGW
                     AYYDGSPAERVTETPYAGRYCVRVPADKSGAKSLWLIAGEAGATYRVSARVRCGPGAE
                     AVVKVAPFRTAFTWAGRYVDIGRASGADWVEVAGQYVVPADAPYISIELSVTGGDAWF
                     DEASVVKSD"
     gene            complement(1843..>2672)
                     /locus_tag="PLQ54_10775"
     CDS             complement(1843..>2672)
                     /locus_tag="PLQ54_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93787.1"
                     /translation="ASLIGFMNALGLGLGASAVWLVLFLLVVVAVTRLRAEYGLPTNE
                     LFRKGPESIMVSSVGSRAFSRQQQVAMALLNWLTRTHRQFPMQVQTDMLEVASRGGIG
                     LSRFGWLVPVLTLVGVVSAVWALLDVSMRVGVATSKVAGPALWAFGPEPWNMASAWIT
                     SPTRGDYRLVGGYVWGVFFCWLLYQIRLVSANWPFHPGGYVMSCTFGLARLWVPLFLT
                     WCFKATVLRYGGHRLYLRFLPLFLGMTAGEYTSAFIRTLIDIAWKLYLPRDSGIGNL"
BASE COUNT          523 a         1046 c          752 g          351 t
ORIGIN      
        1 atgccatctc cgtgcactgc ggcgagactc cgaccgtcga gagctggtgc cgcatccgcg
       61 ccaccggcgg acggccctgg ctgtggatcg aggacggcgc cgagcgctac gacatcgccc
      121 gcgcctacga ttcgcacggc gggcgggcga tcatctactt ccagtccgcc ttctggcgca
      181 acgcgttcct cggcgacgag tcggccgtac ccacccacgc ccagcgcgag gcctggcgcc
      241 tcgcctggct catgctccaa accatgcgcc gggccggcta gtccgacttc accacgctcg
      301 cctcatcgaa ccacgcgtcc ccgccggtca cggacagctc gatgctgata tacggcgcgt
      361 cggccggcac cacgtactgg ccagccacct ccacccagtc ggccccgctc gcccgcccga
      421 tgtccacata ccggccggcc caggtgaacg ccgtcctgaa cggcgccacc ttcaccacgg
      481 cttcggcacc tggcccgcac cgcacccggg ccgacacccg atacgtcgcg ccggcctccc
      541 ccgcgatcaa ccacaacgac ttcgcgccgg acttgtcggc cggcacgcgc acgcagtacc
      601 ggccggcata aggcgtctcc gtcacccgct ccgctggcga cccgtcgtag taggcccagc
      661 cccaccggcg ctccgcgccc gggttgtaga cccgattccc gccctgcgcg gccacgtcgg
      721 cctcggtcag cgatggaatc tcgtcgaaca ccggcaccgg aacctgaagc gcctgcgggg
      781 tcagatccct gtgcggacct gccgtgggac aaaacacgcg ccgcatggcg tccagcgaac
      841 cgaacccata ccgcagcgtc ggctcgacat aggaaccctc accgaactcg ttccaacact
      901 cggcgatgac catgttcagc ttcggatcca cgaactcgag cgagtcccgg cacatgcgct
      961 cgaacagctc gggcgagggg tccgtgcgga ccagcgtgtc ctcaccatac cacggccgcg
     1021 aatcccaacc cgggctgatc ggcgggatgt acggcaggct cgaccgctcc gacacatccc
     1081 gccagatggc gcggatgccg gtcaccatcg tcgcataggg cgcccgccgc tcccccggcc
     1141 ccatgcccgc cgtcgggtag ttgtacgcgc tgaccgcatc gaagccagcc cgggcgacct
     1201 cgtcgagccg ctccgtcgcg ttcgctacca ggaacagtgg gccaatgcct gcctgggcca
     1261 gcacgccgtt catctcccca atgacatccc gcagcccgtc ggccccgaac ccgtcgatca
     1321 gatgcagtgg gcgcgagatc cacaacatgg gacggccatc gatgcgaaga tagttctcac
     1381 gcgccaggta ccgctccaca agcacctcgg ccagcccaac catgtgcgcg cgagtgtagt
     1441 ccggctggcc cggtgactca ttgcaccaca tgaggccaaa ccgcatcagc gggccatacc
     1501 gggagcggag aaacccatca tcgagcgcgg tgttgtggaa actcaccgta cgggtcgtgt
     1561 ggtcatagta ccagtcgaac acgaagaacc gaatgccgtg ctccagcgca aaccggatgt
     1621 gccaatcgga cacctcgggc tcgccgtcgc ggtagtaccc cagcaccggc accggttcgc
     1681 catactcaac catcggccgc catcgaagcg ggttgaagtg cccggggaag tagtagcagc
     1741 ccagcatcac atccccgtgc accggctccg gcggcggcac cggatactcc cccgccgcca
     1801 gcgcaacggc agccagaagc aggctcagca tgacgccccc cctcacaagt tgccaatgcc
     1861 cgagtcgcgg ggtagataga gcttccacgc gatgtcgatc agcgtccgga tgaacgctga
     1921 cgtgtactcc ccggccgtca tcccgaggaa cagcggcagg aaccgcaagt agagccgatg
     1981 gccgccatag cgcagcaccg tcgccttgaa gcaccacgtc aggaacaacg gcacccacaa
     2041 ccgcgccagg ccaaaggtgc acgacatcac gtagccgccc gggtggaaag gccagttcgc
     2101 cgaaaccagc cggatctggt acagcagcca gcagaagaac acaccccaga catacccgcc
     2161 cacgagccgg taatcccccc gcgtcgggct ggtaatccac gcactcgcca tgttccacgg
     2221 ctccggcccg aaagcccaca gggccggccc ggccaccttc gaggtcgcca cacccacccg
     2281 catcgaaaca tccagcagtg cccacaccgc cgacaccacg ccgaccagcg tcagcaccgg
     2341 caccagccag ccgaagcgcg acaaaccgat cccgccccga ctggccacct ccaacatgtc
     2401 cgtctgaacc tgcatcggaa actgccgatg ggtccgcgtc agccagttca gcagcgccat
     2461 cgccacctgc tgctggcggc tgaacgcccg cgaacccaca ctcgagacca tgatgctctc
     2521 cggccccttg cggaataact cattcgtcgg cagaccatac tcggcccgca gccgcgtcac
     2581 cgccacaacc accagcagga acagcaccag ccacaccgcg ctcgccccaa gccccaaacc
     2641 cagggcgttc atgaacccga tcagcgatgc cg
//
LOCUS       DAOSPL010000566         2668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_38312_length_2668_cov_2.880980, whole genome shotgun sequence.
ACCESSION   DAOSPL010000566 DAOSPL010000000
VERSION     DAOSPL010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38312_length_2668_cov_2.880980"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PLQ54_10780"
     CDS             <1..535
                     /locus_tag="PLQ54_10780"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOS93788.1"
                     /translation="IDELMAQTQAVVNGQEPPPPSVYQKRIAFNCIPHIDAFLPDGYT
                     KEEQKMVRETRKILEDETIQVSATTVRVPVYAGHAESVNVEFASPMPATRAREILSGA
                     PGVIVIDDPSVPDYPTPVLAAGTDETYVGRIRDDLTVAHGLNLWVVADNVRKGAATNA
                     VQIAERAIEAGLVTPKA"
     gene            589..1482
                     /gene="dapA"
                     /locus_tag="PLQ54_10785"
     CDS             589..1482
                     /gene="dapA"
                     /locus_tag="PLQ54_10785"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOS93789.1"
                     /translation="MAEMPRLMTAMLTPFDDSLSLDLGRARELAAKLVDEGNDGLVVT
                     GTTGEAPALSHDEKERLWAAVVEAVGSRAMVVAGTGTNSTRDSIELSKVALGAGAHAV
                     MLVAPYYNKPSQEGMYQHFVTAAEAVGAPVVLYNVPPRTASNIEAATVVRAAKAAGNI
                     VAVKEASSDMAQIASIRRNAPPGFVIYPGNDADTLPMMALGALGVISVAGHLVGRDLR
                     MMIDRFHAGDTRGALELHLRMLPIFEACFVPGSPNPCPVKAAMEMIGFGVGGPRLPLV
                     PPTPAQREVIRRALADYGLVT"
     gene            1541..>2668
                     /locus_tag="PLQ54_10790"
     CDS             1541..>2668
                     /locus_tag="PLQ54_10790"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOS93790.1"
                     /translation="MSRQDQLQVIFLGGVGEIGKNMSLLECGDDILVIDCGIGFPEND
                     MLGVDVVIPDYTYLVENRDRIRGMVFTHGHEDHVGGFPFFLRAIGEPDFPVFAAPLTR
                     GIIEAKLSEWEGLPTADFQEVQAGDTVEIGCFTVEFIHVNHSIPAACGLGIRTPLGSV
                     VHSGDFKIDHTPVDGKPLDVERFVAYGDGETLLLIMDSTNAEKPGYVASERGVGDRMQ
                     ALFAEAEGRVIVATFASNIHRMQQVFDISSRLGRKVCVDGRSMLRNIQVASRLGYLNI
                     PPDVRIDLDDVTAYEPRQVTVLTTGSQGEPMSVLVRMSEGEHKHLKTQPGDTVVLSAN
                     PIPGNEALIWRTVNNLCRQGARVIYSAIDNVHVSGHANREEM"
BASE COUNT          486 a          923 c          833 g          426 t
ORIGIN      
        1 catcgacgag ctcatggcgc agacccaggc cgttgtgaac ggccaggaac cgcctccccc
       61 gtcggtctac cagaagcgca tcgccttcaa ctgcatcccc cacatcgacg cgttcctgcc
      121 ggacggttac accaaggaag aacaaaagat ggtgcgcgag acccgtaaga tcctggagga
      181 tgagaccatc caggtctccg cgaccaccgt gcgggtgccc gtctacgccg gccatgcgga
      241 atcagtcaac gtggagttcg ccagccccat gcccgcaacc cgggcgcgcg agatccttag
      301 cggtgcgccg ggcgtcatcg tgatcgacga tccatcggtg cccgactacc cgaccccggt
      361 actggccgcg gggaccgatg agacctacgt gggccgcatc cgtgatgacc tgaccgtggc
      421 ccacggcctc aacctgtggg tcgtagccga caacgtgcgc aagggcgcag ccaccaacgc
      481 ggtccagatt gccgagagag ccatcgaggc tgggctggtc acgccgaagg cctgaaccga
      541 tccggtgacg aaccgcaacc gccaatgaca ccatccggag gtctgaccat ggccgagatg
      601 ccccgcctca tgacggcgat gctcactccc ttcgacgatt ccctctctct ggacctggga
      661 cgtgccagag agctcgccgc caagctggtg gatgaaggta acgacgggct cgtggtcacc
      721 ggcactaccg gcgaagcacc ggcgctgtcc cacgacgaga aggaacgcct ctgggcggcc
      781 gtcgtggagg ccgtcggtag ccgtgccatg gtggtggccg gaaccggcac gaactcgacc
      841 cgagacagta tcgagctctc gaaagtggcc ctgggggccg gcgcccatgc tgtgatgctg
      901 gtcgcgccct actacaacaa gccatctcag gagggcatgt accagcactt cgtcaccgca
      961 gcggaggccg tcggcgcccc ggtggtgctc tacaatgtcc cgccgcgcac ggccagcaac
     1021 atcgaggccg ccaccgtcgt gcgtgcggcg aaggcggcgg gcaacatcgt tgccgtcaag
     1081 gaagcgtcat cggacatggc ccaaatcgct tccatccggc gcaacgcgcc gcctggcttc
     1141 gtcatctacc cgggcaacga cgcggacacc ctgcccatga tggctctggg cgcgctgggg
     1201 gttatcagcg tggccggcca cctggtcggg cgtgatcttc gtatgatgat cgaccggttt
     1261 cacgcgggcg acacccgtgg cgcacttgag ctgcacctga ggatgctgcc catcttcgag
     1321 gcctgcttcg tcccgggctc cccgaacccg tgcccggtga aggcggccat ggagatgatt
     1381 gggttcgggg tgggcgggcc ccgactccca ctcgtcccgc ccactcccgc tcagcgtgag
     1441 gtgatccgcc gggcgttggc ggactacggc ctggtcacct gacgcaccga cggctcacca
     1501 ccgcaccccg accgagccac aaccgaacac ggaggcgccc atgtcgcggc aagaccaact
     1561 ccaggtcatc ttcctcggtg gcgtcgggga gatcggcaag aacatgagtc tcctcgagtg
     1621 cggcgacgac atcctcgtca tcgactgcgg catcggcttc cccgagaacg acatgctggg
     1681 cgtcgatgtc gtcatccctg actacaccta cctcgtcgag aaccgcgacc ggattcgcgg
     1741 catggtgttc acccacggac acgaggacca cgtgggcggc ttccccttct tcctccgagc
     1801 catcggagaa cctgactttc ccgtcttcgc tgccccgctc acccgcggca tcatcgaggc
     1861 gaagctctcc gagtgggaag ggctgcccac ggccgatttc caggaggtcc aagccggcga
     1921 tacggtcgag atcgggtgct tcaccgtcga gttcatccac gtgaaccaca gcatcccggc
     1981 cgcctgcggt ctgggcatcc gcacacccct cggttccgtg gttcactcgg gcgacttcaa
     2041 gatcgaccac acgcctgtgg acgggaagcc actcgacgtc gagcggttcg tggcctacgg
     2101 cgatggcgag acactcctgc tgatcatgga ctcgacgaat gccgagaagc cgggctatgt
     2161 ggcctcggag cgcggggtcg gggaccgaat gcaggccctg ttcgccgagg cagaggggcg
     2221 cgtcatcgtc gccaccttcg cgtccaatat ccaccgaatg cagcaggtgt tcgacatctc
     2281 gtcgcgcctg ggccgcaaag tgtgcgtgga cggccgcagc atgctgcgca acatccaggt
     2341 ggcgtcgagg ctcggctacc tcaacatccc gcccgatgtc cgcatcgatc tcgatgacgt
     2401 cactgcctac gagccgcgcc aggtgaccgt actgaccacc ggcagccagg gcgagcccat
     2461 gtcggtgctc gtgcgcatgt ctgagggcga acacaagcac ctcaagaccc agccgggcga
     2521 caccgtagtg ctgtcggcga accccattcc cggcaacgag gccctcatct ggcggacggt
     2581 caacaatctg tgccggcagg gcgcccgggt catctacagt gccatcgaca acgtgcatgt
     2641 ctcgggccac gccaaccgcg aagagatg
//
LOCUS       DAOSPL010000567         2667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_38331_length_2667_cov_3.316616, whole genome shotgun sequence.
ACCESSION   DAOSPL010000567 DAOSPL010000000
VERSION     DAOSPL010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38331_length_2667_cov_3.316616"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PLQ54_10795"
     CDS             complement(<1..560)
                     /locus_tag="PLQ54_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93791.1"
                     /translation="MTRVLSVSAFVVAYVGLAWASLWCARDGLGYGVWSAPMAVALGG
                     GLLMMLARACSLLAVALRRPVAADLPQRLRVDGHHNVVAASALACVLVIPLYTLMSRS
                     GLALGLRRWEIGVLGLVGAYLVLVAGVVVLDTLAAFARRRPLAYLAATDPEGWLFASD
                     GTAAQRRQAGRRMAGLWRGALGSGVF"
     gene            1409..2329
                     /locus_tag="PLQ54_10800"
     CDS             1409..2329
                     /locus_tag="PLQ54_10800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS93792.1"
                     /translation="MPRAIAQARTADLPDLIRCWNQIMVYDTMSRQEMSRALFCSERH
                     TALVARVDSHTVGLVGCIVPEAGGPTGHITMLCAADEPVATDLLAHAEDLLLGEGVSR
                     VVASEYGGSPLAPGTDPRYAPIRAGFERAGYRHIRDLEDMELCLTDYEPTPHQQRAIE
                     KAAAYGVEVRPWSPELVRRLTRFAGVARASGHLPAGWFWEGWEQGPNMVVALRGEEAV
                     GYARYDPAPRGFYGRYHRRNAGAFGPTGVLADHRGHGVGTWMLAHACLAVKRAGCEWL
                     WAGWTNTPFYIPNEWAVCRRYAVWQKDLGG"
     gene            complement(2468..>2667)
                     /locus_tag="PLQ54_10805"
     CDS             complement(2468..>2667)
                     /locus_tag="PLQ54_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93793.1"
                     /translation="LLLCASVRRAMVTATGIALVSGLVAIGLSYYVRLSVGALAALLA
                     CLTYFAALCWRATSRRRGGVA"
BASE COUNT          474 a          905 c          922 g          366 t
ORIGIN      
        1 ataaagacac cggaccccaa cgcgcctcgc cagagcccgg ccatccgtcg gccggcctga
       61 cgccgttgcg ccgcggtacc gtccgaggcg aacagccagc cctcgggatc ggtggccgcc
      121 aagtaggcca gcggccggcg gcgggcgaac gccgccaggg tgtcgagtac aaccacgcct
      181 gccacgagca ccagatacgc ccccaccagc cccagtacgc cgatctccca ccggcgcagc
      241 cccagcgcca ggccggagcg gctcatcagc gtgtagaggg ggatgacgag cacacacgcc
      301 agcgccgaag ccgcaacgac gttgtggtga ccatccacgc ggagtcgttg cggcaggtct
      361 gcggcgacgg gccgtctcag ggcgaccgcc agcagcgagc aggcacgggc cagcatcatg
      421 aggagcccac cccccagcgc cacggccatc ggggcgctcc acaccccata gccgagaccg
      481 tcgcgagcac accacaggct cgcccaagcg agccccacat acgcgaccac aaacgccgag
      541 acgctgagca cccgcgtcat gctgggcgaa ccactcccat cccgccactt cgaggaccca
      601 ccatagatac caagcatacc atagcactct gctattgttt aggggcactc agcctctggc
      661 gtgtcgaagg acgcgtggtg ccggggcaag cgtcacgacc ggacgcgctg tgggaccact
      721 gcgcgggaga taccgtggta cccccatggt gggagagggt gaaaggtggc tgctgaggaa
      781 acctgcaggg ccgccgcggg tgcctcttgt gcagggttgt ggagggtgtg gagggttgtg
      841 gagcgtttct gggtgttttc ggccggaacg cggcctctcg caaaaaacac gcgagaaccc
      901 tccacaacgc tccacgctcc acacttcagg tggtgaacac gcgcgcgaga ccttccccgg
      961 acaagcggct acgggaggcg actggcgagt agtgtctcgt gacccacctg cgttcgtcga
     1021 gctcccgccc gggtcgggtg tggctcatgg catgctcgcc gttggcttcg gcccaggcgg
     1081 tatgggccga cgggccttct acgcaaactc atgtatgtat tatagtacat gagacaacat
     1141 aagtcaatca aaaggatgta gactaaaggc atgatacgga gcgagcacgg ctcgcccccg
     1201 gtgctggccc caggcgccat gttgaggacc gaggggctga cgcaactcgg ttctgctgcc
     1261 tttggggcgt cagcgccggc cgccgcccgg gcgagtgggt ccgccgatcc ggcgtggggg
     1321 cagcccgcgg ctcaagcgcg gcctcccagt cgccgaagaa cggagaggag ttacacgagt
     1381 attcgcagga cgctcctcgg aggacgccat gccccgcgct atcgcccaag ctcgcaccgc
     1441 tgatctgccc gacctgatcc gatgctggaa ccagatcatg gtgtacgaca ccatgtcgcg
     1501 ccaggagatg agccgcgcgc tcttctgcag cgaacggcac acggccctcg tcgcacgcgt
     1561 cgatagccac acggtgggcc tggtgggatg catcgttcct gaagccggcg ggcccaccgg
     1621 gcatatcacg atgctgtgcg ccgcggacga gccggtggcg accgacctgc tggcgcacgc
     1681 agaggatctg ctcctcggcg agggagtctc ccgggtggtg gcctccgaat acggtggcag
     1741 ccccctggct cccggcaccg acccgcgtta cgcgcccatc cgcgcaggat tcgagcgggc
     1801 cggctaccgc cacatccgtg atctggagga catggaactc tgtctcacgg actacgagcc
     1861 gactccacac cagcagcggg ccatcgagaa ggccgcagcg tacggggtcg aggtgaggcc
     1921 ctggtctcct gaactcgttc ggcggctcac tcgcttcgcg ggtgtggcgc gcgcctcggg
     1981 ccacctaccc gcgggctggt tctgggaagg ctgggagcag ggcccgaaca tggtcgtcgc
     2041 ccttcgcggc gaggaagctg tcggctacgc ccgctacgac ccagcgccga gaggtttcta
     2101 cggccgctac caccgccgca acgccggggc gttcggcccg actggagtgc tggccgatca
     2161 ccgaggccat ggtgtgggca cctggatgct ggcccacgcc tgcctcgcgg tgaaacgcgc
     2221 gggctgcgaa tggctgtggg ccggctggac caacacgcct ttctacatcc cgaacgagtg
     2281 ggcggtctgc cgccggtacg ccgtgtggca gaaggacctg gggggctgaa cgcgacgatg
     2341 atcccctcgc ccggcccggc agggcggtgg ggcccacgtg ggcctctggg ggcggggagc
     2401 ccagggtcga gggctcgaga cgcgggctgg ggaagccctg gcccgtgggt gcggagccgc
     2461 tgcgcgctca cgccacgcct ccgcggcggc gcgaagtggc ccgccagcac agagcggcga
     2521 agtaggtcag gcatgcgagc agggcggcca gagcacccac agagaggcgg acatagtagg
     2581 acagcccgat agcgacgagg ccggacacga gggcgatgcc ggtagcggtc accatggccc
     2641 ggcgcaccga cgcgcacaac agcaggg
//
LOCUS       DAOSPL010000568         2666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_38350_length_2666_cov_3.279203, whole genome shotgun sequence.
ACCESSION   DAOSPL010000568 DAOSPL010000000
VERSION     DAOSPL010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38350_length_2666_cov_3.279203"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..2426)
                     /locus_tag="PLQ54_10810"
     CDS             complement(138..2426)
                     /locus_tag="PLQ54_10810"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOS93794.1"
                     /translation="MAEHVETLFEQRFDGAALPEGRWQLDAGAELAADGLHTRSIADQ
                     GGNIGAHMVEGIPIPAASEGCFLRVEWSLVPVKLGGWGQDIRTESGPFVVEFTGTRPT
                     LNAKVPARDTVTEGQETRLSCDFGRNAVFSWTIDGQEQLDGPVPAWSGGSQAKLSLQE
                     FKDTNSESRWLWVRISRVTPDDVLPLRLSEWDDLSGVRADEPSAFLVGHASPMDKVFR
                     EAGDFGGRLTGPARISAAGRERESFQLVVMPLGRPVEGAEVRVSDLLHADGHTRLTAD
                     HVAWHPVGYVQTKQSNSSIRRAGWWWPDVLMPPAPFDVAPGFVQPVWFTVDVPDGTPP
                     GVYRGLIDVTARDCPPHTVGLELTVQPYSLPVRGKLRTAFCISAGLWEIWYRPDEVKR
                     RLGMTDQTNHGPLYSSYECEDVLPQAKWREVYDFLLAHRISPTTIYSGMKNGRTRVVP
                     SREDMDYCYERGMNATCLANVDVLPADATAADRYMAELEAWLGEWDAFIRERNWPDFT
                     WYVHGFDESDMRPNHETEVDPSIRRIFGMIHERYPSIKCESANPVNPAHAGLFDIITP
                     VTAQWRPSDAERQAAGDELWAYVCCGPGKPYANLFIDFPGVDPRILGWQFYQHNLTGF
                     LYYLMNLYEGQENWNMEGPKWPDRPWNPLSFGTNGDGILFYPGPDGTPLASTRMENLR
                     DGIEDYEALAVLSGLADRLRAAGGHEELLARCADVLKVRPEVSRSWTEYTQEPDRIIA
                     ARAEVDELIAAILAVPEVATVE"
BASE COUNT          428 a          938 c          854 g          446 t
ORIGIN      
        1 tgggtgtgcg tgggtgccga ccctaagtgc gtgccattcg tgactgtcac cggttcccgc
       61 ctcctgccct tcgctgaccg cagtctgcga acaccgcccg cgcagagcct cgggatggca
      121 cccggggctt ccactcctca ctcgacggtc gccacctcgg gcacggccag gatggcggcg
      181 atcagctcat cgacctcggc ccgagccgcg atgatccgat cgggctcttg ggtgtactcg
      241 gtccacgacc tcgagacctc gggcctgacc ttcagcacgt cggcacaccg cgcgaggagc
      301 tcttcgtgcc cgccggcggc tctcagccgg tcggcgagac cgctcagcac agccagagcc
      361 tcgtagtcct cgatgccatc acgcaggttc tccatgcggg tgctggccag aggggtgccg
      421 tccgggccgg ggtagaacag gatgccgtcg ccgttcgtgc cgaaggatag cgggttccac
      481 ggacgatcgg gccacttggg gccttccatg ttccagttct cctggccctc gtacaggttc
      541 atcaggtagt agagaaaccc cgtcaggttg tgctggtaga actgccagcc gaggatgcgt
      601 gggtcgacac cggggaagtc gatgaacagg ttcgcgtagg gcttccccgg cccgcagcag
      661 acgtaggccc acagctcgtc gccggccgcc tgccgctcgg catcggacgg tcgccactgg
      721 gccgtcactg gcgtgatgat gtcgaagagc cccgcgtggg ccgggttgac ggggttggcc
      781 gactcgcact tgatcgacgg ataccgctcg tggatcattc cgaagatgcg gcggatggag
      841 gggtcgacct ctgtctcgtg gttgggccgc atgtcggact cgtcgaaccc atgcacatac
      901 caggtgaagt ccggccagtt gcgctcccgg atgaaggcgt cccactcgcc aagccaggcc
      961 tcgagctcgg ccatgtaccg gtccgcggcc gtcgcgtccg cgggcagcac gtccacattc
     1021 gccaggcacg tcgcattcat gccgcgctca tagcagtagt ccatgtcctc ccggctcggc
     1081 acgacccgcg tgcggccgtt tttcatcccc gaatagatcg tcgtcgggct gatgcgatgg
     1141 gccaggagga agtcatatac ctcgcgccac ttggcctgcg ggagcacatc ctcgcactca
     1201 taggatgagt agagcggccc gtggttggtc tggtcggtca tgccgaggcg ccgcttgact
     1261 tcatcgggcc ggtaccagat ctcccacagc cccgcactga tgcagaaggc cgtccggagc
     1321 ttcccccgga cgggtagtga gtagggctgc acggtcagtt cgagccccac ggtgtgcggc
     1381 gggcagtcgc gagcggtcac atcgatcagc ccgcgataga caccgggcgg cgtaccgtcg
     1441 ggaacgtcca cggtgaacca gacgggctgg acgaaaccgg gagccacgtc gaacggtgcc
     1501 ggtggcatca gcacgtccgg ccaccaccag ccggcccgcc ggatggacga gttcgactgc
     1561 ttcgtctgca cgtaacccac cgggtgccac gccacatggt ctgcggtaag ccgcgtgtgg
     1621 ccgtcggcgt gcagcaggtc ggagacccgc acctcagcgc cctcgaccgg cctcccgagt
     1681 ggcatgacga cgagctggaa gctctcgcgc tcgcgtccag ccgcggagat gcgcgccggg
     1741 ccggtcagcc gcccgccaaa gtcgcccgcc tcgcggaaga ccttgtccat cgggctggcg
     1801 tggcccacga ggaatgccga aggctcatca gcccgaactc cggataggtc gtcccactcg
     1861 ctcagccgca agggcagcac gtcatccggt gtgacccggc tgatacgcac ccacagccac
     1921 cgcgactcgc tgttcgtgtc cttgaactcc tgcaggctga gcttggcctg actgcctccg
     1981 gaccacgccg gcaccggccc gtcgagctgc tcctggccat cgatggtcca tgagaacacc
     2041 gcgttccgac caaagtcgca ggaaagtcgg gtctcctggc cctcggtcac cgtgtcccgc
     2101 gccggcacct tcgcattcag agtcggccga gtcccggtga actcgaccac gaagggcccg
     2161 ctttcggtgc ggatgtcctg cccccagccg ccgagcttca ccggaacgag tgaccactca
     2221 acccgcagga agcacccctc ggacgctgcc gggatcggga taccctccac catatgggca
     2281 ccgatgttgc ctccctggtc ggcgatgctg cgcgtgtgga gcccgtccgc agcaagctcc
     2341 gccccggcgt cgagctgcca ccgcccctcg ggcagtgccg ctccgtcgaa gcgctgctcg
     2401 aacagcgtct ccacgtgctc cgccatggct gcactcgccc ccagaagccc gattgcgagt
     2461 agtgcggttg gccgcatggt cccgcccccc cagggccgcc gaccaccgtc acggcccaac
     2521 actgcataca tagccctggt gcgggtcgga tggcgccctt cccgcttcgc aggccgcttc
     2581 cattcggtcc atccactgac catgacctgc actgcgcatg aggatgtgag gcataagaga
     2641 cacagctggg gagtcggtgg atccga
//
LOCUS       DAOSPL010000569         2655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_38565_length_2655_cov_2.815385, whole genome shotgun sequence.
ACCESSION   DAOSPL010000569 DAOSPL010000000
VERSION     DAOSPL010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38565_length_2655_cov_2.815385"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2206
                     /locus_tag="PLQ54_10815"
     CDS             <1..2206
                     /locus_tag="PLQ54_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93795.1"
                     /translation="ATGLITIPVADEGSGIRPESIRVAVSGQEFTAADPAVRIGPDSA
                     SVQVDLASALKSIANGQVVEVALTHVEDRIGHSAAQPRPAEVKLTFSADKDPPDTPRI
                     ISGGSCLTTDTFESASPRDMGQWQAIRGSGAEIALDDTTAASGRRSLRVYCTDSRGAF
                     QTYIRREPFDAGKYRVISFDYRIDPWMRIDLIIQTGDGVYRLLKMTDNDGEYPSLGRA
                     IDPKTADGQWHHAEINLHAMLAKRIRKPSGFIIRSVRIGGTGWPGNPRGSSLHIDNFS
                     VFGVTSARQGLSMVWQAEDASGIAGASYVVDDHPDTVPPETVVTQETSAVCPGVPDGT
                     AYFHLRLVDGAGNWGKPIHHACIIDNDGLTIGEPTPAPGAVACTSEIRIPLTDQGTSG
                     VDPASLKLVVAGQEFTVENGALEYLPTPGELIWRGQNATPQPVALPDKSTVTVKLADA
                     RDFAGNGPDQPLEYQWTMDFSLDTEAPPAPRVSSVTHPTAFFEPFEGTPEQGALGECR
                     GSAGTQIAIDTTTKASGDSSLKITFAEGKVFRAEMRHTPFDVERNPKVGFDYCVPPGV
                     QVDLLFLLEGGQQVLVNFTDNERLARSRVPGVIADGKWHSVRNFDLKAAARQVAGNAE
                     GLVCTCVAFIDAGQPETPLGSSFWIDNFVVYREGSTAPRLSWKASDPTGIQGFSYVFD
                     DNPGTIPDDTLEGTDTSLRLDPPKAGVHFFHIRALDGSGRWSPTAHYTVVQR"
     gene            complement(2239..>2655)
                     /locus_tag="PLQ54_10820"
     CDS             complement(2239..>2655)
                     /locus_tag="PLQ54_10820"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOS93796.1"
                     /translation="WPTPTYHVFYMFRPHMGANAFVADVDGPTVEYAAADGARPSMPA
                     VHASASARADDGAMCVTLANRHLTDDVECRIALPGGTIGEANAIQLAGATADAHNTAD
                     APDSVRPVEAHVERSGNEVRVILAKCSVAAIQLRVG"
BASE COUNT          463 a          928 c          872 g          392 t
ORIGIN      
        1 ggccacgggc ctgatcacca tcccggtcgc cgacgagggg tcaggcatcc gccccgaatc
       61 gatccgtgtc gcggtcagcg gccaggagtt cacggccgcc gaccccgccg tccgcatcgg
      121 cccggacagc gcctccgtgc aggtcgacct cgcctcagcc ctcaagtcca tcgccaatgg
      181 ccaagtcgtc gaggtggctc tgacacatgt ggaggaccgc atcggccact cggcagcgca
      241 gccgcggccg gccgaggtga agctgacatt ctccgccgac aaggacccgc cggacacccc
      301 aaggattatc tcgggcggca gctgtctgac gacggacacc ttcgagagtg cttcgccacg
      361 cgacatgggg cagtggcagg cgatccgcgg gagcggggcc gagatcgcgc tcgatgacac
      421 caccgcggcg tcggggcgcc gcagcctgcg cgtgtactgc accgactcgc gcggcgcctt
      481 ccagacctac atccgacgcg agccattcga cgcgggcaag taccgcgtca tcagcttcga
      541 ctaccgcatc gatccatgga tgcgcatcga tctgatcatc cagacaggcg acggggtcta
      601 tcgcctactc aagatgaccg acaatgacgg cgagtaccca tcgctgggcc gcgccatcga
      661 ccccaagacc gccgatggcc aatggcacca cgctgagatc aacctacacg ccatgctggc
      721 caagcggatc cgcaagccct cgggctttat catccgatcg gtacggattg ggggcaccgg
      781 ctggccgggt aacccgcgcg ggtccagcct ccacatcgac aacttctccg tgttcggggt
      841 caccagcgcc cggcaggggt tgtccatggt ctggcaggcc gaggacgcgt ccggcatcgc
      901 cggagccagc tacgtcgtcg atgaccatcc ggacaccgtc cctcccgaga cggtggtgac
      961 gcaggagacg agcgccgtgt gcccgggcgt ccccgacggc accgcgtact tccacctgcg
     1021 gctggtggac ggggccggga actgggggaa gcccatccac cacgcgtgca tcatcgacaa
     1081 cgacggactg accatcgggg agcccacacc agcgcccggg gcggtggcat gcacgtccga
     1141 gatccgcatc ccgctgaccg atcagggcac ctcgggcgtc gatccggcct cgctgaagct
     1201 cgtagtggcc ggacaggagt tcaccgtcga gaacggcgca ctcgagtatc tcccgactcc
     1261 cggcgagctg atctggcgcg ggcagaacgc cacaccgcag cccgtggcgc tcccggacaa
     1321 gtcgacggtc acggtgaagc tggcggatgc gcgcgacttc gccgggaacg ggcccgacca
     1381 gcccctcgaa taccagtgga ccatggactt ctcactcgac acggaggcgc cgccagcccc
     1441 gagggtgtcg tcggtcaccc atcccaccgc cttcttcgaa cccttcgagg gcacaccgga
     1501 gcagggcgcc ctgggcgagt gtcgcggcag cgccgggacg cagatcgcga tcgacaccac
     1561 caccaaggcc agcggcgaca gcagcctcaa gatcaccttt gccgagggca aagtgttccg
     1621 ggccgagatg cgccatacgc cctttgacgt cgagaggaac ccgaaggtcg gcttcgacta
     1681 ctgcgtgcca cccggagtcc aagtcgatct gttgttcctg cttgagggtg gccagcaggt
     1741 gctggtgaac ttcaccgata acgagcggct cgcccggtca cgcgtcccgg gcgtcatcgc
     1801 cgacggcaag tggcatagcg tccggaactt cgacctgaag gccgccgccc ggcaggtggc
     1861 gggcaacgcc gaggggctcg tctgcacctg tgtggcattc atcgacgccg gccaacccga
     1921 gacgccgctg ggatcgtcgt tctggatcga caacttcgtc gtataccggg aggggagcac
     1981 ggcgccgagg ctgagctgga aggcgtcgga tcccacgggc atccagggct tcagctacgt
     2041 gttcgacgac aaccccggaa ccattcctga tgacacactc gagggcaccg atacatcgct
     2101 gcgcctcgat ccgccgaagg cgggagtgca cttcttccac atccgcgccc tcgacggatc
     2161 gggtcgctgg agccccacgg cgcactacac cgtggtgcag cggtaggtct ggggccaggc
     2221 gccgcgaggt ggcgctactc agcccacccg caattggatt gctgccaccg agcacttggc
     2281 cagaatgacg cgcacctcgt tgccgctgcg ctcgacgtgc gcctcgaccg gcctgaccga
     2341 atcgggcgca tcggccgtgt tgtgcgcgtc ggccgtggcg cccgccagct gaatagcgtt
     2401 cgcctcacct atggtgcctc ccgggagcgc gatgcggcac tcgacatcat ccgtgaggtg
     2461 ccggttcgcc agggtgacgc acatcgcccc gtcgtcggcg cgggccgagg ccgacgcgtg
     2521 gaccgccggc atagaggggc gggcaccgtc ggctgcggcg tactcgacag tggggccgtc
     2581 gacatcggcc acgaacgcgt tggcccccat gtgcggccgg aacatgtaga acacgtggta
     2641 agtgggtgtc ggcca
//
LOCUS       DAOSPL010000570         2651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_38632_length_2651_cov_4.158320, whole genome shotgun sequence.
ACCESSION   DAOSPL010000570 DAOSPL010000000
VERSION     DAOSPL010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38632_length_2651_cov_4.158320"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="PLQ54_10825"
     CDS             <1..1216
                     /locus_tag="PLQ54_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93797.1"
                     /translation="AEGEGRWVEDPAVIHESAFNVIGLRPQTTYEFEASLHASSGRKV
                     GQSGPVTARTDERRLREWRGLVLHEPMRLRIPEGAFPSIQAYRGRFELAYSVGGAIVL
                     TELDADLRPLSTREIVPPHPVGDGYAVQGNLQSCRVGSRLYVSFKRDHAPFVASARLC
                     VVEVDLDSGAVRGPAVIESSRLGRSPWNGGIAAVRDEVWIGYADTRDEFGDRTSEIVL
                     RRFDPRVMEADSEEFRLGTAPSPYLYAPLLGSFGNTIAVSFSDVTEESPTAAKAQIEN
                     EPLYVCLFDGRRFTDPVPIELQGRHRYGQMIQVGAKLLVAWKRGASYAAVEWGPYMFH
                     DIGLALVDPLKRTVEITTYVDDLKYNAYPAMARDGDRIVLIHTKHEHDYGIAGDPAAF
                     LGFWAGWIGTGQ"
     gene            complement(1236..>2651)
                     /locus_tag="PLQ54_10830"
     CDS             complement(1236..>2651)
                     /locus_tag="PLQ54_10830"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOS93798.1"
                     /translation="ALLYMVNRIAEKVEAEFPDVLVCTLAYLETAKPPKTMRPRSNVA
                     MQLCTDNCMWAHPFTPARSIPAFREAMEGWSAICDKILIWDYCVNFSHYTAPMPNMDA
                     IADNIRYFVAHSAAGVMEQGAYQSVGAERDAMRSWVFAKLMWDPTRDLRALERDFILG
                     YFGKAGPPILEYNELLRKAGEEHAEALASPEGGIRYGMDSPFLTRELLDRADALYDQA
                     ERQADSPEILARVQRDRLPILYVKLCRGPQFVGSGYARLIDEFERIARQVGLTHILEG
                     GPDVDAKLAHWRESARVDPTRLHGAPLASEWRFRIDPDDRGAAEKWYAPETNDSDWAI
                     VRSDTGTGWESQGFAGYSGYGWYRQQFEVEPEVLQQPSLRLFFGAVDEQAWVYINGQL
                     AFEHTVASTGLPIETLWTTPFGFDPRPFLRPGTNTIAVRVHDVLGMAGIWRPVTLIWG
                     EMDYSPAMLWELVQGKFGTSK"
BASE COUNT          460 a          830 c          903 g          458 t
ORIGIN      
        1 ggccgaaggc gaggggcgat gggtcgagga tccggccgtg atccacgaga gcgccttcaa
       61 cgtgatcggg ctgcgcccac agacgaccta cgagtttgag gcatccctgc acgcgtcatc
      121 gggcaggaag gttggacaga gcgggccggt gacggcccgg accgacgagc ggcggctgag
      181 ggagtggcgc ggcctggtgc ttcacgagcc gatgcggcta cgcattccgg aaggcgcctt
      241 cccctccatc caggcttatc gaggtcgttt tgagctggcc tacagtgtcg gcggcgcgat
      301 tgtgctcaca gaactcgacg ccgacctgag gcccctctcg acgcgagaga tcgtgccgcc
      361 gcaccctgtt ggggacggat atgcggttca gggcaacctg cagagctgcc gggtgggctc
      421 gcggctgtat gtgagcttca agcgcgatca cgcgcccttc gttgccagcg cgaggctgtg
      481 cgtggtcgag gtggacctgg actcgggcgc agtccggggc ccggcggtga tcgagtcgag
      541 ccggctgggg cggtcgccgt ggaacggtgg catcgcggcg gtgagggatg aggtctggat
      601 cggctacgcc gacacgcggg atgagttcgg cgatcgcacg tcagagatcg tgctgcggcg
      661 cttcgaccct cgggttatgg aggccgactc ggaggagttc cggctcggga ccgcgccttc
      721 gccgtacctc tatgcaccgc tcctgggaag cttcggcaac accatcgccg tgtcattctc
      781 agacgtgaca gaggagagcc cgacagcggc caaggcccag atcgagaacg agccgctcta
      841 cgtctgtctg ttcgacggcc gacgtttcac cgatccggta cccatcgagc tccagggccg
      901 gcaccggtac ggccagatga tccaggttgg ggccaagctc ctggtggcat ggaagcgcgg
      961 ggcctcgtac gcggcggtgg agtgggggcc ctacatgttc catgacatcg gattggctct
     1021 ggtggacccg ctcaagcgca ccgtcgagat cacgacctat gtcgatgacc tcaagtacaa
     1081 tgcctacccg gccatggcgc gagacggcga tcggatcgtg ctgatccata ccaagcacga
     1141 gcacgactac ggcatcgccg gcgacccggc ggccttcctc ggcttctggg ccggatggat
     1201 cggcacaggg cagtagagta ggcgcccggg tcggcctact tgctcgtccc gaacttgcct
     1261 tgcaccagtt cccacagcat cgcggggctg tagtccatct cgccccagat cagcgtcacg
     1321 ggccgccaga ttccggccat gcccagcacg tcgtggaccc gcacggcgat ggtgttcgtt
     1381 cccggcctca ggaagggcct cgggtcaaag ccgaatggcg tggtccagag cgtctcgatc
     1441 ggcaagcccg tgctggccac ggtgtgttcg aaggcaagct gcccgttgat atagacccac
     1501 gcctgctcat cgacggcgcc aaagaacagt cgcaggctgg gctgctgaag cacctcgggc
     1561 tcgacctcga actgctggcg gtaccaccca tagccgctgt aaccggcgaa gccctgggac
     1621 tcccagccgg taccggtgtc cgaccgcacg atcgcccaat ccgagtcgtt cgtctcgggc
     1681 gcgtaccact tctcggcagc accccggtcg tcgggatcga tgcgaaagcg ccactcgctg
     1741 gcgagcggcg cgccatgcag tctagtggga tcgacccggg cggactcacg ccagtgtgcc
     1801 agtttggcgt ccacatcggg gccgccttcg aggatatggg tcagcccaac ctgacgagcg
     1861 atgcgctcga actcgtcgat gagcctcgca tagcccgaac cgacaaactg gggcccacga
     1921 cagagtttca cgtacaggat aggcagccgg tcgcgctgaa cgcgcgccag aatctcgggc
     1981 gaatcagcct ggcgctctgc ctggtcatag agggcgtcgg ctcggtcgag cagctcgcgg
     2041 gtcaggaagg ggctatccat gccgtaacgg atgcctccct cggggctggc cagcgcctca
     2101 gcatgctcct cgcccgcttt acggagcagc tcgttgtact cgaggatggg gggacccgcc
     2161 ttgccgaagt agcccaggat gaaatcacgc tcgagggcac gcaggtcgcg ggtggggtcc
     2221 cacatcagct tggcgaacac ccaggagcgc atggcatcgc gctcagcgcc gacactctga
     2281 taggcgccct gttccatgac gcccgcggcg ctgtgggcga cgaagtagcg aatgttgtcg
     2341 gcgatggcat ccatgttggg catgggcgcc gtgtaatggc tgaagttcac gcagtagtcc
     2401 cagatgagga tcttgtcgca aatggcgctc cagccctcca tggcttctct gaacgcgggg
     2461 atgctacggg ccggcgtgaa tgggtgggcc cacatgcagt tgtccgtgca gagctgcatg
     2521 gccacattgg accggggcct catggtcttg ggcggcttgg cagtttcgag gtaggccagg
     2581 gtgcagacga gcacatcggg gaactcagct tcgaccttct cggcaatgcg attgaccatg
     2641 tagagcaacg c
//
LOCUS       DAOSPL010000571         2651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_38636_length_2651_cov_3.426810, whole genome shotgun sequence.
ACCESSION   DAOSPL010000571 DAOSPL010000000
VERSION     DAOSPL010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38636_length_2651_cov_3.426810"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /locus_tag="PLQ54_10835"
     CDS             complement(<1..1413)
                     /locus_tag="PLQ54_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93799.1"
                     /translation="MGRAALGALLVAWGVLVGGCGAGTGAGGMATAGAAFRFGNAVNL
                     PFATVASGPIDALLVEEPASASDRVSVRGASTESYAGAQSLHRFLASVGARPAGSRLL
                     LGRRLIHTRDGELVLPNRDAVARGLARAGNGDLTFAYDGWSPAAQAELSAFVDAIYPV
                     CVQVYGKPAFSNTVTLVLDDTITDLTGGVYDATANEIRIPALTDNADANHVMIVRQLL
                     HAFHDDAMFYYEQWEQGFSVAAADAIVSIYNPAYDPSYGSLYVTNLYELLNTPELGHD
                     RIFGTGFDGMITWRLGMAAAAWSKCWVEDPTFFARFNDAYYARWNADATAAGNVPVLA
                     RIASSVLPSVEGQSFYEWFRRQYALDTSLDVGDRTYIYSLPEHDRLILVIDTFVATED
                     GRETPRSGGAVLEFFDYTAAFSLYVQEGYEVSIPGVGTDAGQGYYVGSLYNIGGAQRI
                     TVTVAIGSVQRQLIFPYMVRG"
     gene            1690..2085
                     /locus_tag="PLQ54_10840"
     CDS             1690..2085
                     /locus_tag="PLQ54_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93800.1"
                     /translation="MDAPDHRAPLGWVFVVVGIAAIVGGMTARREPAAPESGDEALLA
                     AMGSRERIEGRLRAEAKDLLEWEMLSTGEPVRSGRIVIPVSPEGLGAVTIEQPKSPRP
                     GTMIVSGIILIGIGTILKLFAQPSPPPVA"
     gene            2121..2618
                     /locus_tag="PLQ54_10845"
     CDS             2121..2618
                     /locus_tag="PLQ54_10845"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOS93801.1"
                     /translation="MSDPGSQTDARQAMERILASQSVGHLGLTDAGEVYVVPLNYTYS
                     RGRILFHCALEGRKLDMIRANPRVCFEVSCQGAEPLPHGGDPCDTPFESVICWGTARI
                     VEDLSERRAILDEFQMRFEKPGGPREPVPMARVERCGAVEIAVSRMTGRRGLGHRPER
                     WEWVA"
BASE COUNT          473 a          839 c          926 g          413 t
ORIGIN      
        1 tccccgcacc atgtagggga agatgagctg ccgctggacg gagccgatcg ccacggtgac
       61 cgtgatgcgc tgagcgccgc caatgttgta cagcgacccc acgtagtagc cctgcccggc
      121 atcggtgccc acgccgggga tggagacctc gtagccctcc tgcacataca gggagaacgc
      181 ggcggtgtag tcgaagaact cgagcacggc gccacccgag cgcggcgtct cgcggccgtc
      241 ttcggtggca acgaaggtat cgatgacgag aatcagccgg tcgtgttcgg ggagtgagta
      301 gatgtaggtg cggtcaccga cgtcgaggct ggtgtcgaga gcatattggc gacggaacca
      361 ctcatagaag gattgccctt cgaccgaggg cagcacgctc gacgcgatcc tcgccagcac
      421 aggcacgttc ccggcagcgg tagcgtcggc gttccaccgg gcgtaatagg catcattgaa
      481 ccgggcgaag aaggtggggt cctcgaccca gcacttggac caggcagccg cagccatccc
      541 gagccgccac gtgatcatgc cgtcgaaccc ggtgccgaag atgcggtcat gcccgagttc
      601 cggggtgttg agcagctcgt aaagattcgt cacgtacaat gagccatagc tggggtcata
      661 ggccgggttg tagatgctga cgatggcatc ggccgccgcg acggagaacc cctgctccca
      721 ctgctcgtag tagaacatgg cgtcgtcgtg gaaggcgtgc aggagctgcc ggacgatcat
      781 gacatggttc gcgtcggcat tgtcggtgag cgcggggatg cggatctcat tggccgtggc
      841 atcgtacacg ccgcctgtga gatccgtgat ggtgtcatct agaaccaggg tcaccgtgtt
      901 gctgaaggcg ggtttgccgt agacctggac acagacggga tagatggcat cgacaaaggc
      961 actcagctcg gcttgggccg ccgggctcca gccatcatag gcgaaggtca gatcaccatt
     1021 acccgcacgc gccaaccctc tcgccacggc gtcgcggttg gggaggacga gctcgccgtc
     1081 acgagtatgg atgaggcggc gtcccagcag gagccgggaa ccggctggcc tggcgcccac
     1141 cgacgcgagg aaccggtgga gcgactgcgc gccggcatag gactccgtgg aagcccctcg
     1201 aacggacaca cggtctgagg ccgatgcggg ctcctcgacg agtagggcat cgattgggcc
     1261 cgaggccacc gtggcgaacg gcaagttgac cgcgttgccg aagcgaaagg cggcgcccgc
     1321 agtggccatg ccgcccgctc cggttcctgc cccacagccg cccaccagca ccccccaggc
     1381 gaccagaagg gcaccgagcg cggcccgtcc catgaccggg cctcgcaaca cacccatgtg
     1441 gcaccgcctc gcgcacatcg cttcgctgat cttcacgagt tagacgtccc gctcaccgcc
     1501 ctcgtctcag tcagacggta ccacaggacc gagggttcag gcacggacct ccaggggcat
     1561 ggacgcccag ttggggccac ggggcgcggc ccgcaccctg accggctccg agcggacggt
     1621 ggtgcggtcg attggtgctg acctgagggg ccaccaggcc atatactggg gccgcagcgg
     1681 aggtgagcca tggacgcgcc ggaccatcga gcgcctctcg gctgggtctt cgtcgtcgtg
     1741 ggcattgcgg cgatcgttgg gggaatgaca gcccggcggg agcctgcggc accggagagc
     1801 ggcgacgagg cgctgttggc ggccatgggc agccgcgagc ggattgaggg ccgtctccgc
     1861 gccgaggcca aggacctgct cgaatgggag atgctgtcga ccggggagcc ggttcgctcg
     1921 ggccgcatcg tgatcccggt atcgcccgag gggctggggg cggtgaccat cgaacaaccg
     1981 aagtctccga gaccgggaac catgatcgtc agcggcatca tactgatcgg catcggcacg
     2041 atcctcaagc tgttcgcgca gcccagtccg ccgccggtcg cctagatcca tcggccgact
     2101 cacatacgga gggccgctcc atgtcagatc cagggtcaca gaccgatgcc aggcaagcca
     2161 tggagcgtat cctggccagc cagtccgtcg ggcatctggg tctcaccgac gccggtgagg
     2221 tctatgtggt gccgctgaac tacacgtact cgcggggacg gatcttgttc cactgcgcgc
     2281 tggagggccg gaagctcgac atgatccgcg cgaatccccg ggtctgtttc gaggtgtcct
     2341 gccagggcgc agaacccctt cctcacgggg gcgatccatg cgacacgccc ttcgagagcg
     2401 ttatctgctg gggtacggcc cggatcgtcg aggacctatc ggaacggcgg gccatactcg
     2461 acgagttcca gatgcgcttc gagaagcctg gcggtccacg ggagccggtc cccatggccc
     2521 gtgtcgagcg ctgtggggcg gtggagatcg ccgtctcgcg catgaccgga cgccggggac
     2581 tggggcatcg gcccgagcgg tgggagtggg tggcatgagc gccgcccagc aggcggggcc
     2641 atgaggcggt c
//
LOCUS       DAOSPL010000572         2644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_38755_length_2644_cov_3.322518, whole genome shotgun sequence.
ACCESSION   DAOSPL010000572 DAOSPL010000000
VERSION     DAOSPL010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38755_length_2644_cov_3.322518"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="PLQ54_10850"
     CDS             <1..1209
                     /locus_tag="PLQ54_10850"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOS93802.1"
                     /translation="MHPWIGLWPSAYAVRAYDVLYALSALCFAAMALRLTRRQGLPAP
                     LAMDLSAAYVLAMTVGATLAYDLVYHQPWSLRAFRLGGVIEGGLYGGPAIALALIVAL
                     AASMRWPVSALDMGTVCLALGLTVAKLGCLMHGCCYGRPTGSPWAMVFPPGSYAPAGV
                     PLVPTQALDAALLAATALVLGVAHLRQRARPGWLAVWFVLLFCAGRFVTEFGRDEARS
                     PGLTMAQWALLAGLLGAAGLLSVAPAPWARLAVWLDARSTRRAAPVEPRGPRLLVRAG
                     ASAVDAALVAIWAVAAGPLLACVVAGIVFGLAPLAMGATPGMRVFGLRVVAEFGARPR
                     GRALLRGALLPLSVLSLFGCLRPLFDPQRRALHDVLSGTRVVPAAGASRSRSEDADSA
                     PAGMTEVVRG"
     gene            1216..2466
                     /locus_tag="PLQ54_10855"
     CDS             1216..2466
                     /locus_tag="PLQ54_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93803.1"
                     /translation="MTRGPCWRCRGRGHACVALGLAWLALASLAATAHSAPTVYVLPL
                     RADVDSEAGSEWLGYLLSDVANRALGGVARVPTSDDVERATRSQRQALGLIDTANEAA
                     LAGAEMACQFVAYGSLRVLGEDAVVDVEVLHVASGAPVVQQSIRGATRDLEDLVSRVL
                     ERVTVGAGLEPVGLPALKTALPATESPQAVRHLFAADHLWDRAVGAEAAGLQDLAHES
                     LTECIATLGRAIEADPAFGWAYERLGRATRHVLALDPKNGPAYGNLALVCRYTGDYVG
                     AVAALREAVRLVPNDYHLQLSLGNALVDVALASDAASRLAPLTEAQSVFRAALEVAPD
                     DPACHINLAATLYYLGNLDEAGDEYARAAALEPSNAAAFLGVGLSRARMGRVAEAQTA
                     FRQAMALDPQGPIGRRAQRELGGE"
BASE COUNT          283 a          928 c         1008 g          425 t
ORIGIN      
        1 atgcatccgt ggatcggtct ctggccgagc gcttacgctg tgcgcgcgta cgatgtgctc
       61 tatgccctct ccgcgctgtg cttcgccgcc atggcccttc ggctgactcg gcgccagggg
      121 ctgcccgccc cgctcgccat ggacctgtcg gccgcgtacg tcctcgccat gaccgtgggg
      181 gcgaccctcg cctacgacct ggtctaccac cagccgtggt cgctgcgggc gttccggctc
      241 ggcggcgtga tcgaaggcgg cctctatggc ggcccggcca tcgccctggc gctcatcgtg
      301 gcgctggccg cgagcatgcg gtggccggtg tccgcgctgg acatgggcac ggtctgcctg
      361 gcgctgggcc tgactgtggc gaagctggga tgcctgatgc atggatgctg ctacggcagg
      421 cccacgggca gcccgtgggc catggtgttc ccccccggct cctatgcccc ggcgggcgtg
      481 ccactcgttc cgacgcaggc ccttgatgcg gcgctcctcg ccgccacggc tctggtactg
      541 ggggtcgccc acctccggca gcgggcacgg ccgggatggc tcgccgtgtg gttcgtgctc
      601 ctgttctgcg ccggccggtt cgtcacggag ttcgggcggg atgaggcccg gagcccgggg
      661 ctgaccatgg cccagtgggc gctgctggcc gggctgttgg gcgccgcggg cctgctaagc
      721 gtggcgccgg ccccgtgggc acgcctggcc gtgtggctgg atgctcggtc aacccggagg
      781 gcagcgccgg tcgagccgcg aggccctcga ctgctcgtgc gagccggggc gtctgccgtc
      841 gatgccgcgc tcgtggcgat ctgggcggtg gcggcgggcc cgctgctggc ttgcgtcgtc
      901 gccggcatcg tgttcggtct cgcgcctctc gccatgggcg ccacgcccgg catgcgagtg
      961 ttcgggctgc gggtcgtggc cgagttcggg gccagacccc gtgggcgggc gctgctgcgc
     1021 ggcgcgctcc tgccgctctc cgtgctctcg ctgttcggct gcctccggcc gctgtttgat
     1081 ccacagcggc gggccctgca cgatgtgctc agtgggacgc gggtggtgcc ggccgcaggg
     1141 gccagccggt cgcgctcgga agacgctgac tcggcgccag cgggcatgac ggaagtcgtg
     1201 agagggtgag catcggtgac gcgggggcct tgctggaggt gtcgagggag gggacatgcg
     1261 tgcgtcgccc tcgggctggc gtggctcgcg ctggcatcgc tggcggccac ggcccactcg
     1321 gcccccacgg tctacgtgct gccgctgcgg gccgacgtgg acagcgaggc gggctcggag
     1381 tggctcgggt acctgctgag cgatgtggcc aaccgcgcgc tgggtggggt ggcccgggtg
     1441 cccaccagtg acgacgtgga gcgcgccacc cgctcgcagc ggcaggcgtt gggcctgatc
     1501 gacaccgcca acgaagccgc tttagccggc gcggaaatgg cctgtcagtt cgtcgcctac
     1561 ggatcgttgc gcgtgctggg cgaggacgcc gtcgttgatg tggaggtact ccatgtggcc
     1621 tccggcgcgc ccgtggtgca gcagagcatt cgaggcgcga cccgcgatct ggaggacctg
     1681 gtgtcgcggg tgctggagcg ggtgaccgtc ggggccgggc tcgagccggt ggggcttccg
     1741 gcgctcaaga ccgccctgcc ggccaccgag tcgccccagg ccgttcgtca cctgttcgcg
     1801 gccgaccatc tgtgggaccg cgcggtgggg gcagaggcag ccgggctcca ggacctggcg
     1861 cacgagtcgc tgaccgagtg catcgccacg ctgggccgag ccatcgaggc cgacccggcc
     1921 ttcggctggg cctacgagcg cctgggccgt gcgacacggc atgtgctggc gctcgacccg
     1981 aagaacggtc cggcctacgg caatctcgct ctcgtgtgcc ggtacacggg cgactacgtg
     2041 ggcgccgtgg ccgcgctgcg ggaggccgtc cgcctcgtgc ccaacgacta ccatctccag
     2101 ctcagcctcg gcaatgcgct ggtcgatgtg gcgctcgctt ccgatgcggc gtcgcggctc
     2161 gcgccgttga ccgaggccca gtccgtcttc cgcgccgcac tcgaggtggc gccggacgat
     2221 ccggcctgcc acatcaacct ggcggcgacg ctgtactacc tcgggaatct cgacgaggcg
     2281 ggggacgagt acgcccgggc ggcggcgctg gagccgtcga acgccgccgc gttcctgggc
     2341 gtgggcctca gccgggctcg catgggccgt gtggccgagg cccaaaccgc cttccgccaa
     2401 gccatggcac tggaccccca gggccccatc ggccgccgcg cccagcgcga gctgggtggg
     2461 gagtgatcgg gcttcagcgc ccgccggcac gggacgtggt cgctcgtgcc ctaccgctgc
     2521 ggagctggcg ctccgcgctc cgcacccccc ccggaagttg gggccccaga tccccattgg
     2581 cggtaacaca gggcccttgg ccgggcggct gggtctcgcg aaggtcgcgc ggaacgcccg
     2641 cctg
//
LOCUS       DAOSPL010000573         2642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_38786_length_2642_cov_3.751836, whole genome shotgun sequence.
ACCESSION   DAOSPL010000573 DAOSPL010000000
VERSION     DAOSPL010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38786_length_2642_cov_3.751836"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1292
                     /locus_tag="PLQ54_10860"
     CDS             147..1292
                     /locus_tag="PLQ54_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93804.1"
                     /translation="MNRVLAILSAGVLVLSGAEYLSGPPSSGQWAVQRLILPALAYGT
                     LMGVTGVPRAWLWTGLGAALYVSAQSVGVLLDFGIPVDATRFLVVPVTSLFAAFGQSI
                     ARQRREARVLREVAERLLSARWNPGTLARRSAAVPGVDALDLDTSVLTEGGLIASVLA
                     EDRRASSSSRVSCYVLAAILIVLYLMTQSLLLLGMGLGVFVVTALSLLWTTLTRATSP
                     FDALVAGYFELAAVGFGTALRAHPLDVKSRIGLGLALLHLRRIDQAVHELSTARRIGL
                     HRFSIAVWVGIERVLAVAAYEKGDHNRAIRAFESVLALYPDHAPTLYYTGLAHAASGR
                     RRMARDLLGRAAALGYDEAARALATLPEQPPRREARPAPDAVIEEPA"
     gene            1327..2427
                     /locus_tag="PLQ54_10865"
     CDS             1327..2427
                     /locus_tag="PLQ54_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS93805.1"
                     /translation="MKVADWRARDEGWSPDVSRLQAALWCRQPDRVPLAELKVDDTVK
                     AAFLGLDQWPADRRGAMAADVEFARAAGYDAVRATAQVDYGATGHKKRHHYSANTEAE
                     QERTWQQLGLGPIRALDDVEAYPWPDPEAADVTDLELASELAPPGMGIITALKGGGIF
                     ERAWYLLGFEGFMTATVENPELVAVTMERAGRVWYEVARRCLGFPRVSVLWFSDDLAY
                     TEGFMVNPSIYRAHLFPWIERLGDLCRRRMPPVPLVFHSDGKLWDVLGDLMACGVNGL
                     HPIEPKAMDIVEVKRRVQGRMCVIGNIDLGYTLTRGTPAEVEAEVRERIRTVGPGGGY
                     CLGSSNSVTDYVPVANFAAMARSCLRYGRYPL"
BASE COUNT          365 a          852 c          981 g          444 t
ORIGIN      
        1 caggggtagc ctccctcgcg gtgtgcgggc catcgagcgg ccgcagcgga gcatccggca
       61 aaggtggcct cgagggcgcg ggcgaagtcg cccccagcat cgggagtggc cgtgcccccg
      121 cgaggtgagg tggcctggga gcctccgtga accgagtgct ggcgatcctg agcgccggcg
      181 tgctggtcct gtcgggcgcg gagtacctgt ccgggccgcc gagttccggg caatgggcgg
      241 tgcagcgcct gatactgcct gctctcgcct acgggacgct catgggcgtc acgggtgtgc
      301 cgcgggcctg gctctggacg ggactgggtg cggcactgta cgtgtccgct cagtccgtgg
      361 gtgtcctcct agactttggc attccggtcg atgccacccg cttcctggtc gttcccgtca
      421 cgagcctgtt cgccgccttc gggcagtcga tcgcgcgtca gcggcgcgaa gcccgcgtgc
      481 tccgtgaggt ggccgagcgg ctcctgtcgg cgcggtggaa cccggggaca ctggctcggc
      541 gatcggcagc ggtacccggt gtcgatgcgc tggacctgga cacttcggtg ctgacagagg
      601 ggggcctgat cgcgtcggtt ctcgccgagg accgccgcgc atcctcgagc tcccgggtct
      661 catgctatgt gctggccgcc atcctgatcg tgctctatct catgacccag tcgctcctac
      721 tgctcggcat gggtctgggg gtcttcgtgg tgaccgcgct gagcctgctg tggacgacgc
      781 tcacgcgggc cacatcgccg ttcgacgctc tggttgcggg gtacttcgag ctggcggctg
      841 tggggttcgg cacggcactc cgggcgcatc cgctggatgt gaagtcgcgg atcggcctcg
      901 gactcgccct actccacctc cggcggatcg accaggcggt gcacgagctc tcgacggccc
      961 ggcggatcgg cctgcaccga ttctccatcg cggtgtgggt gggcatcgag cgcgtgctgg
     1021 ccgtggcggc ctatgagaag ggggaccaca atcgagcgat ccgtgcgttc gagagcgtcc
     1081 tggctctgta ccccgatcat gcgcccacac tgtactacac aggtcttgcg cacgcggcgt
     1141 ccgggcgacg gcggatggcc cgtgatctcc tcgggcgggc ggcggccctg gggtacgatg
     1201 aggccgctcg agccctggcc acactgccgg agcagccgcc ccgccgtgag gcacggccgg
     1261 cgcccgacgc ggtgatcgag gaaccggcct gacgagagtc atgcgccgac gggggaacga
     1321 ccaggcatga aggtcgcaga ttggcgagcg cgcgatgaag gctggagccc cgacgtgtca
     1381 cggctgcagg ccgctctgtg gtgccggcag cccgatcggg ttccgctggc tgagctgaag
     1441 gtcgacgaca cggtcaaggc ggcgttcctg ggcctggatc agtggccggc ggaccggcgg
     1501 ggagccatgg cggccgacgt cgagttcgcg cgggcggcgg gctacgatgc cgtacgcgcc
     1561 acggcccagg tcgactatgg agccacgggc cacaagaagc gccatcacta cagcgccaac
     1621 accgaggccg aacaggagcg cacatggcag cagctcggtc tgggcccgat tcgcgcgttg
     1681 gatgatgtcg aagcgtaccc ctggcccgat cccgaggcgg ccgatgtgac cgacctcgag
     1741 ctggcgtccg aactggcgcc gccgggcatg gggatcatca ccgcgctcaa ggggggcggc
     1801 atcttcgaac gcgcgtggta cctcctgggg ttcgagggct tcatgaccgc cacggtcgag
     1861 aacccggagc tggtggcggt caccatggag cgtgccggcc gggtgtggta cgaggtcgcc
     1921 cgacgatgcc tgggcttccc ccgcgtgtcg gtgctgtggt tctcggacga cctggcctac
     1981 accgagggct tcatggtgaa cccgtccatc tatcgcgcac acctgttccc gtggatcgag
     2041 cgtctcggtg acctgtgccg acgccggatg ccaccggttc ctctggtgtt ccactcggac
     2101 ggcaagttgt gggatgtgct cggcgatctg atggcgtgcg gggtgaatgg actgcatccc
     2161 atcgagccga aggccatgga catcgtcgag gtgaagcgcc gcgtccaggg gcggatgtgc
     2221 gtgatcggca acatcgacct ggggtacaca ctgacgagag gcacgccggc cgaagtcgag
     2281 gccgaggtgc gggagcgcat tcggaccgtg ggtcccggcg ggggctactg cctgggctcg
     2341 agcaacagcg tgacggacta cgtgcccgtg gccaacttcg cagccatggc gcgctcgtgt
     2401 ctgcggtatg gccggtaccc actgtagggc agccgcttcg ggcttcgccg cgctgttctg
     2461 ggcttccgca aggggtgaca cccgtgctga gcccggtgct ggcggcggcg acgttactgg
     2521 gagcgcgtcc catagacact cgcacagggc ccttggccgg gcggctgggt ctcgcgaata
     2581 tcgcgccgaa cgcccgcctg gtcttgtgga ggggccgcac aggggtgcgc ccagagggcg
     2641 cg
//
LOCUS       DAOSPL010000574         2642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_38788_length_2642_cov_3.183610, whole genome shotgun sequence.
ACCESSION   DAOSPL010000574 DAOSPL010000000
VERSION     DAOSPL010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38788_length_2642_cov_3.183610"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            340..1584
                     /locus_tag="PLQ54_10870"
     CDS             340..1584
                     /locus_tag="PLQ54_10870"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOS93806.1"
                     /translation="MGRLALTGGSAVRTRPFPSWPVSDEREVEAVTQVVRSGKWWYGE
                     KVREFEAAFAAFQGATFGVSATSGTTALEIALLACGVGAGDEVIVPPYTFMATASAVL
                     RVNGVPVFADIDLATSNLDPSDVEQRITDRTKAIIPVHFGGLPVDMDAFSALASRHGL
                     RIIEDACHSWGSQWRGKGTGALGDCGVFSFQMSKNITSGEGGIMLTDNEELADQARGF
                     SNCGRSKTGGWYEHFLLGSNLRMTELQAALLLVQLSRLEEQTERRQANARLLNRRLAA
                     IPGIALIDWDDPRVTRRSYHIYAFRFLRDQWGGVTRDRFLEALNAEGIPSSAGYPIPL
                     YKNPLFQRQADGPAGCPVSCPYYQGHVDYTQAVCPNAETLCEEACWIFHQILLGDASD
                     MDDIAGAIEKVWEHRSELAQAF"
     gene            1609..2451
                     /locus_tag="PLQ54_10875"
     CDS             1609..2451
                     /locus_tag="PLQ54_10875"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOS93807.1"
                     /translation="MIDFHLHIGRMYREDYPARPALGPDQLVDWMNRVGIDVGVLLPL
                     ESPEGSWGYFLTEDAVAARDQYPDRLIAFVCVDPRYPNAARLIRRAVEDWGCKGFGEH
                     VCGLAFDDERNLALYAVCDELELPLDFEISTDLCWDEVGLPRLERCLKQFPHIRWCGH
                     GPGFWSAISGDDPRVGYPPGPVTPGGALDRLLSEYDHLYADISAGSGYNALTRDPDFT
                     AGFVERHWRKLLFGTDYLCHLDRPPILRWMQDVSVPAEVRDAIAFGNARQVLGLAPPA
                     LAVT"
BASE COUNT          423 a          837 c          885 g          497 t
ORIGIN      
        1 catctggcac tgcctgctgt cgttcggagc gcctgaccgg agcctatggc ctcggaatcg
       61 agcatagggg cgggaaccgg tgacggtctc gaatggtacg tgcctggctt ctgacagggc
      121 ccgacggccg cgcacgacca cccgtaggcg gggcttcagc ctcgcgcgga cccgctcccg
      181 atggcgttat gatccgaggc tcaagcctcg gctacaggat ctgatgggcg tacctgagcc
      241 cgcggggctc atgtgcgtat catccgtgag tgtctcaggt ctctgcaacg tgccggtggc
      301 cgttgcctca gcagttggca tagaaggagc gtgcaatcga tgggcagact cgcactgacc
      361 ggcggatccg ccgtgcggac gcggcccttc ccatcctggc cggtctctga tgagcgtgag
      421 gttgaggccg tcacccaagt cgtccgcagt ggcaagtggt ggtacgggga gaaggtgcgc
      481 gagttcgagg cagcctttgc ggccttccag ggcgccacat tcggggtctc ggccaccagt
      541 ggcaccacgg cgctcgagat cgcgctcttg gcttgtggcg tcggtgccgg ggatgaggtc
      601 atcgtccccc cctatacctt catggccacc gccagcgccg tgctgcgcgt caatggggtg
      661 ccggtcttcg cggacattga cctggcgacg agcaatctgg acccatccga tgttgagcag
      721 agaatcaccg atcggactaa ggccatcatc ccggttcatt tcggtggcct gccggtggat
      781 atggatgcct tttcggccct ggcctcaagg cacgggcttc ggatcatcga ggatgcctgc
      841 catagctggg gcagtcagtg gcgcggcaag gggaccggcg ccctgggcga ctgtggtgtc
      901 tttagcttcc agatgagcaa gaacatcacg tccggcgagg gcgggatcat gctcaccgac
      961 aacgaggagc tggccgacca ggcccgcggc ttctcgaact gtgggcgatc caagacgggc
     1021 ggatggtatg agcacttcct cctgggctcc aatctgcgga tgaccgaact ccaggcggcg
     1081 ctcctgctcg ttcagctctc gcgcctcgag gagcagaccg agcggcggca agccaacgct
     1141 cggctgctga atcggcgcct tgccgccatc ccgggcatcg cactcatcga ttgggacgac
     1201 ccgcgggtta cgcggcggag ctatcacatc tatgccttcc ggttcctccg tgaccagtgg
     1261 ggaggcgtca cccgcgaccg gttcctcgag gcgctgaatg cggaaggcat tccctcgtca
     1321 gcggggtacc ccatccctct ctacaagaac ccgctcttcc agcgccaggc cgacggaccg
     1381 gccggctgtc cggtgtcgtg cccctactac caagggcatg tcgactacac gcaggccgtg
     1441 tgccccaatg ccgagaccct ctgcgaggaa gcctgctgga tcttccacca gattctcctg
     1501 ggagacgcgt cggacatgga cgacatcgcc ggtgccatcg agaaggtctg ggagcatcgg
     1561 tccgagcttg cccaggcttt ctgattgcgc ggcagcggag gtatgttagt gattgacttt
     1621 cacttgcaca tcggccgcat gtaccgtgag gactatccag cgcggccggc actggggccg
     1681 gaccagctgg tcgactggat gaaccgtgtc ggcattgacg tgggcgtgct gttgccgctg
     1741 gaaagccccg agggttcgtg gggttacttc ctgaccgaag acgcagtggc ggcacgggat
     1801 cagtacccgg accgcctcat tgcctttgtc tgtgtcgacc cgcggtaccc gaacgcggct
     1861 cggctgatcc gtcgcgcggt ggaagactgg ggctgcaagg ggtttggcga gcacgtgtgt
     1921 ggactcgcct tcgatgatga gcgcaacctc gccctctacg cggtgtgcga tgagctcgag
     1981 ctgcccctgg acttcgagat cagtaccgac ctctgttggg atgaggtcgg gctgccgcgc
     2041 ctcgagcgtt gcctcaagca gtttccccac atccggtggt gtggccatgg gccagggttc
     2101 tggagcgcga tctcgggcga cgaccctcga gttgggtatc cacccgggcc tgtaacgcct
     2161 ggcggagcac tggaccggct cctcagtgag tacgatcacc tctatgccga tatctcggcg
     2221 ggctcaggct acaacgcgct cacccgcgac cctgacttca ccgcagggtt cgtcgaacgt
     2281 cattggagga agctgctgtt cggcacggac tacctatgtc atctcgaccg gccgccgatc
     2341 ttgcggtgga tgcaggacgt gagtgtgccg gctgaggtgc gtgacgccat cgcctttggc
     2401 aacgcgaggc aagtgctggg gttagctccc ccggcgctgg cggtgactta gggctctggc
     2461 ggcccatggg ccgggttgcg gagcggcgcc acgccgcgtt cgggtgcgct ccatcggcca
     2521 tgcgcccacc gggtccttcg accggctcag gacaggggcc cctccagcag acaggggagg
     2581 gaactgcggt gtacgggcct tccaagcacc gcccgggccc aggtcaccgc caacaagctt
     2641 cc
//
LOCUS       DAOSPL010000575         2641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_38811_length_2641_cov_3.170147, whole genome shotgun sequence.
ACCESSION   DAOSPL010000575 DAOSPL010000000
VERSION     DAOSPL010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38811_length_2641_cov_3.170147"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLQ54_10880"
     CDS             <1..213
                     /locus_tag="PLQ54_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93808.1"
                     /translation="GPVPGPVSIDPYPQLRLYPCLLIGVHYRKSLRGLHGDQVAGVDV
                     VAAYGHETGALPPNCDNDKTLRRRAS"
     gene            310..1305
                     /locus_tag="PLQ54_10885"
     CDS             310..1305
                     /locus_tag="PLQ54_10885"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS93809.1"
                     /translation="MSDAVTGSARGDGRRYAFGPVVSRRLGRSLGVDLVPYKTCSYDC
                     IYCQLGRTTRLTAVRSQFAPLARVVEDALRALEISGPADYITLSGSGEPTLCTRLDEL
                     IAALKARTGVPIAVLTNGSLLFRPEMRRELAGADLVVPSLDAGTAEAFQRVNRPDPSI
                     GFEAMVDGLIEFGQDYGGRIWLEVFLVRGYTDSLDHVGRIAALARQIGPERVQLNTVA
                     RPPAEGDALPVDPAVLDDLAGAFGALAEVVAERPAVERAAGGGAEVQRLLDLLARRPC
                     HLLAMAEALNAHPNAILKVLPGLVREGKVTVTRHGGRDHYAATHPSGEHGPVEWV"
     gene            1324..2178
                     /locus_tag="PLQ54_10890"
     CDS             1324..2178
                     /locus_tag="PLQ54_10890"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOS93810.1"
                     /translation="MSQGSMTDQQAQGECPAMSRDEQYEQQEQRLRAQSALIRHKILV
                     MSGKGGVGKSTLATNLAWSLAGRGHQTGLLDADINGPSIPLMVGLQDRVGTGTVGFEP
                     VQVLPNLGVLSIAFFLNSPDTPTIWRGPLKGGVIRQFVADVEWGPLDYLVIDLPPGTG
                     DEPLTVAQMFPDADGGVIVTTPQEASLSVCRKAVNFLHSVKLPCLGIVENMSGFVCPH
                     CGQATDVFARGGGEQMAKKMRVPFLGRVPLSAEIVSRADQGLPLVGADAPAAVREAIE
                     GIAVLLGE"
BASE COUNT          406 a          780 c          988 g          467 t
ORIGIN      
        1 gggccggtac ccggcccggt ctccatcgac ccgtaccccc agcttcgcct ctacccctgt
       61 ctactcattg gggtgcacta caggaagagc cttcggggtc tccatgggga ccaggtggcc
      121 ggggtggatg tagtggcggc gtatgggcat gagacaggtg cgttaccgcc aaactgtgat
      181 aacgacaaga ccctgcggag acgggcgagt tgacagccgc ggcagtatgc ctgattatat
      241 gcgtcagaca tatagtcatc gcagcgggcc gttcgtcacg cgacccggga gccagtggtg
      301 ggattgtccg tgtccgacgc ggtaactggg tctgcccgtg gggatggtcg gcggtatgcg
      361 tttggccccg tagtgtctcg tcgcctgggg cgctcgctcg gagtcgacct cgtcccctac
      421 aagacctgct cgtatgactg catctactgc caactggggc gcacgacacg gctgacagcc
      481 gtccggtcgc agttcgcgcc cctggcgcgc gtggtcgagg atgcactgag ggcactggag
      541 atctcggggc cagcggacta catcacgttg tcaggttcag gcgaacccac tctctgcacg
      601 cggctcgatg agctgatcgc cgcactcaag gcccggacgg gagtccccat cgcggtgctc
      661 acgaacggct cgctgctctt ccggccggag atgcgccggg agttggctgg cgcggacctg
      721 gtggtgccgt cgcttgacgc ggggacggct gaggcattcc agcgcgtgaa ccggcctgac
      781 ccgtcgattg ggttcgaggc gatggtcgac ggcctgatcg agttcggcca ggactatgga
      841 gggcgcatct ggctcgaggt gttcctggtt cggggctaca ctgacagcct ggaccatgtg
      901 gggaggattg ccgccctggc gcggcagatc ggcccggagc gcgtgcagct caacacggtg
      961 gcccggccac ccgccgaggg ggatgcgctg ccggtggacc cggcggtgct cgacgacttg
     1021 gctggcgcgt ttggggctct ggcggaagtg gttgccgagc ggccggcggt ggagagagcc
     1081 gccgggggcg gggcggaggt tcagcgcctt ctcgacctgc tggcccgtcg gccctgccat
     1141 ctgctggcaa tggctgaggc cctgaatgcc cacccaaacg cgatcctcaa ggtgctcccc
     1201 gggttggttc gcgaggggaa agtgacggtc actcgacacg gtggccgcga ccactatgcc
     1261 gcaacgcacc cgtccggcga acatgggccg gtggaatggg tatgagtatg aagaggggat
     1321 ccgatgtcac aaggctcgat gacagaccaa caggcccagg gcgaatgccc cgccatgagc
     1381 cgcgatgagc agtatgagca gcaggagcaa cggctacgcg cgcagtcggc actgatccgt
     1441 cacaagattc tcgtcatgag cggcaagggc ggggtcggca agagcactct ggcgacgaat
     1501 ctggcctggt ctctggccgg gcgcggtcac cagacggggc tactggacgc ggacatcaac
     1561 gggcccagca tcccactgat ggtcgggctg caggaccgag ttggcacggg cacggttggg
     1621 ttcgagcccg tgcaggtgtt gccgaacctc ggcgtgctgt ccatcgcgtt cttcctgaac
     1681 agcccggaca cgccaacgat atggcggggg ccgctgaaag gcggtgtgat ccggcagttc
     1741 gtggccgacg tcgagtgggg cccgctcgac tacctggtca tcgatctgcc gccgggcacg
     1801 ggtgatgagc cactcaccgt tgcccagatg ttccccgatg ccgatggcgg cgtgatcgtg
     1861 acaacgcccc aggaggcgag cctgtcggtc tgccgcaagg cggtcaactt cttgcactcg
     1921 gtcaagctgc cgtgcttggg catcgtggag aacatgagcg ggttcgtgtg tccgcactgc
     1981 gggcaggcca ccgatgtgtt cgcccgcggc ggcggcgagc agatggccaa gaagatgcgc
     2041 gtcccgttcc tgggacgtgt gccgctgtcg gcagagatcg tgagccgggc cgaccagggc
     2101 ttgcctctgg tgggcgccga tgctccagcg gccgttcgtg aggccatcga gggcattgcg
     2161 gtgctgttgg gcgagtaggg ccggcggggc gaacgagcct ggttgtggcg ccacggacgg
     2221 cttcatctcg ttgcccgggc gcgatttggg gcccgtctct gagtggcgtc tgccgcacgg
     2281 gggatccagt cctccatcct ggcctgggtt atgcatgaag cataggtgtc aggtgggtat
     2341 ccgatttgga gcgttgcgga atcgggtgtt ggggtgcgga ggggaggccg gaaccggtgg
     2401 agggggctgc agcggtcggc tggggcagtc tgcgatggtt gtggaggcga ggtcacgcgg
     2461 tcgccccggc gccgtgggcc gcgtcgatcc ccatgtttgt ccatggggag gcagtgggga
     2521 gagagcactt ggcccggcga acacctgggt ggacgtgggc ggcgacctgg ggggccgcat
     2581 cggcgcgtcg ggtgttgggg tcgttgacat cctcatagcc cgcggccagg ccgaagatgc
     2641 g
//
LOCUS       DAOSPL010000576         2638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_38870_length_2638_cov_3.137437, whole genome shotgun sequence.
ACCESSION   DAOSPL010000576 DAOSPL010000000
VERSION     DAOSPL010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38870_length_2638_cov_3.137437"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..2386)
                     /locus_tag="PLQ54_10895"
     CDS             complement(170..2386)
                     /locus_tag="PLQ54_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS93811.1"
                     /translation="MDTTTQLPDEIRAAVDSLDGSLGSVRAIVETDVGTDGGYGSAWL
                     VVADKRVLVYPSGNGAHPSIDLDMEAIKGAAVTPLVGVSAIELTTPSGRVELVRYSNA
                     KAKRFAKVCKIIDSLANGRDVPEDALVDEDRVCATCKRELPEDTDVCPACVRKSKVVR
                     RLLGYARPYIGRCVLVFVLMIAAVAAGLVPPYLTRYLVDHVLKEPQFYGKLGLMVLAL
                     TGSHVLGLALSIWRGRVAGWLGNRLVRDLRAEVYRALNRLSMSYFDKKQVGAVMARVI
                     RDTGMLADFLIDGALWFMVCALEIIGIGIVLFCMDWKLALLILIPAPLISLVTYVFWR
                     RIRSTFHKVWHQWSAMNAALNDTFAGIRVVKAFAQEQREVSRLDVRNDKLFVVGARSE
                     QILATFFPLLYSVGGAGFLLVWYFGGRGVQNGGITLGTLMAFLGYLGMFYGPLQSLSR
                     MGDWLSRCLTSGERIFEVIDTEPEAYDDPEAIPMPEMKGRVEIRDVRFSYEPGKKALD
                     GISIDVEPGEMIGLVGHSGAGKSTLINLICRFYDVDEGAILIDGVDVRKIRLHDLRRQ
                     IGIVMQEPFLFPGTIAENIAYGKPEATLDEIMAAARTANAHDFIMKSPYGYDTEVGER
                     GKLMSGGERQRISIARAILHDPKILILDEATSSVDLETEKNIQEALARLTSSRTTFAI
                     AHRLSTLRNANRLLVLEKGKVAELGSHDELVKKRDGVYAKLAKTYRDLSKLRAVDG"
BASE COUNT          477 a          871 c          829 g          461 t
ORIGIN      
        1 gcccacggcc aggtacgacc gatcgccctc gatggtcatg tcgggccgcg cctggccgcg
       61 ggggatgagc gtgatcttcg acggctccag gtacttcagt tccaccggct cggccttcgc
      121 gcgcggcatc acggcctcgg cttccgatga gctcatatgc ttcccccccc tacccgtcca
      181 ccgcgcggag cttcgagaga tcgcggtagg tcttggcgag cttggcgtag acaccatcgc
      241 gcttcttcac gagctcatcg tggctaccca gctcggccac cttgcccttc tcgaggacca
      301 gcaggcggtt cgcgttccgc aacgtcgaca gccggtgggc gatggcgaag gtcgtgcgcg
      361 atgaagtgag ccgagccagc gcctcctgga tgttcttctc agtctccagg tccaccgacg
      421 aggttgcctc gtccagaatc aggatcttcg ggtcatgcag gatggcgcga gcaatggaga
      481 tcctttggcg ctcgccgccg gacatgagct tgccgcgctc gcccacctcg gtgtcgtaac
      541 catagggtga cttcatgatg aagtcgtgcg cgttcgccgt tctcgcggcc gccatgatct
      601 catccagcgt tgcctccggc ttcccgtagg cgatgttctc cgcgatggta cccgggaaca
      661 ggaagggctc ctgcatcacg atgccgatct gccgccgcag gtcgtggagc cggatcttgc
      721 ggacgtcaac tccgtcgatg aggatggcac cctcgtcgac gtcgtagaag cggcagatga
      781 ggttgatgag ggtcgacttt cccgcgccgc tgtgcccaac caagccgatc atctcgccgg
      841 gctcgacgtc gatggagatc ccgtccagcg cctttttccc cggctcgtag ctgaagcgca
      901 cgtcccggat ctccacacga cccttcatct cgggcatcgg gatggcctcg ggatcgtcat
      961 aggcctcggg ctcggtgtcg atgacttcga agatccgctc gccggaagtc aggcaacgcg
     1021 agagccagtc gcccatgcga ctcagcgact gcagcgggcc atagaacatg ccgaggtaac
     1081 cgaggaatgc catcagcgtc ccgagcgtga taccgccatt ctgcacgccg cggccgccga
     1141 agtaccagac gagcaggaat ccggcaccgc ccaccgagta gagcaggggg aagaaagtgg
     1201 ccaggatctg ctcggaccgg gcacccacca cgaacagctt gtcgttgcgg acatcgagcc
     1261 ggctcacttc acgctgctcc tgcgcgaagg ccttcaccac gcggatgccc gcaaaggtgt
     1321 cgttgagcgc ggcgttcatg gccgaccatt ggtgccagac cttgtggaac gtgctgcgga
     1381 tccggcgcca gaacacatag gtaaccagtg agatcagtgg cgcggggatc agaatcagca
     1441 gcgcgagctt ccagtccatg cagaacagca cgatgccgat gccgatgatc tccagcgcgc
     1501 agaccatgaa ccagagggcg ccatcaatca gaaagtcggc cagcatgccg gtgtcgcgga
     1561 tgacccgggc catgacggcg ccaacctgct tcttgtcgaa gtaggacatg gatagccggt
     1621 tgagggcacg gtacacctcg gccctcagat cgcgaaccag ccgattcccc agccagcccg
     1681 ccacccggcc ccgccagatc gaaagtgcca gcccgagcac atgcgacccc gttaacgcca
     1741 gcaccatgag gccgagtttg ccgtagaact gaggttcctt gagcacatgg tcgacgaggt
     1801 agcgggtcag gtagggcggc acgagccctg ccgcgacagc cgcgatcatc agcacgaaca
     1861 cgagcacgca tcggccgata tagggccggg cgtagcccag cagacgacgg acgaccttgc
     1921 tcttgcgcac gcaggcgggg cagacatcgg tgtcctcggg cagctcccgt ttgcaggtgg
     1981 cgcagacgcg gtcttcatca acgagcgcgt cctcggggac gtcgcgtccg ttggccaagg
     2041 agtcgatgat cttacagacc ttcgcgaagc gtttcgcttt cgcgttcgag taccgcacta
     2101 gctcgacccg gcccgaaggt gtggtgagtt cgatcgccga gacgccgacg agtggcgtca
     2161 cggccgctcc cttgatggcc tccatgtcca ggtcgatcga cggatgcgct ccattgccgc
     2221 tggggtacac gagcacccgc ttgtccgcca cgacgagcca agcggaccca taaccgccgt
     2281 cggtgcccac atcggtctcc acaatggcac gaacggagcc gagggagccg tccagggagt
     2341 cgacagccgc tctgatctcg tctgggagtt gtgtcgtggt atccaactac gggttcctct
     2401 tcagatgacg gcacgcacgt ccacgcgggg agaggggcgg gtccgaaccg gcaccctcta
     2461 tactatagcg cggcgtgaat gggcattaca gggccgaggg cactcggagc cgcaccctcg
     2521 cctattggat ctgcctccac ccgcggttcc gcagcgcgtt ctcggtgtcc gggtcgttgg
     2581 gcacccggtg catcacgcca tcggggtcct tccacacgcc gccgtcgcgg ttgggcac
//
LOCUS       DAOSPL010000577         2635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_38920_length_2635_cov_3.358527, whole genome shotgun sequence.
ACCESSION   DAOSPL010000577 DAOSPL010000000
VERSION     DAOSPL010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38920_length_2635_cov_3.358527"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="PLQ54_10900"
     CDS             complement(<1..1011)
                     /locus_tag="PLQ54_10900"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOS93812.1"
                     /translation="MRNARAIVVVLDGVGVGALPDAAAYGDEGAHTLAHTAQAVDGLA
                     VPHLAAMGLGHIATVLGVPRSPAPGSHHGRLTEISAGKDTITGHWELMGVVTSVPFPT
                     YPAGLPGRLLDEFARRIGRPVLGGQVASGTEIIAELGGEHLKTGCPVVYTSADSVFQI
                     AAHEGIVPVDQLYQWCQIARGMLQPPDHMARVIARPFAGEPGAFVRTPRRRDFALPSP
                     PNQLDAVAAAGGEVRGVGKVDDMFAGRAFSTCRHVGSNAEGITALMDALASDFAGVVL
                     VNLNDTDTRYGHRNDPEGFAASLEEFDAAVPALLARLRPDDLLIITADHGCDPTTPST
                     DHT"
     gene            complement(1004..1828)
                     /locus_tag="PLQ54_10905"
     CDS             complement(1004..1828)
                     /locus_tag="PLQ54_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS93813.1"
                     /translation="MRYLRFYVLVVALVGGLWSFWYYEGHRVIRAFESSAHYIQGLQI
                     TQDESLTPQTRQRLAGAAFGRALATSPSPFETGLRVAAAYERARMYEDALTIYDAIIA
                     DATPSLGLYLQAAQCAYLLGDVHRAEPYYRAAMRIAPDDPTAYNGLGYLYAEQGVHLG
                     QAEQLVLRALQLHEARRPEGGSVFLGRLDSQRFRLERATFQDSLGWVYFKQGRYEEAL
                     EQLRAAASVLGAHPEVRYHLAAAEAEAGDQPPPRPDHSPAPKGRPGPPDRGTRLDA"
     gene            complement(1914..>2635)
                     /locus_tag="PLQ54_10910"
     CDS             complement(1914..>2635)
                     /locus_tag="PLQ54_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOS93814.1"
                     /translation="PRVLIGIPSGVTEVEQRAVLEAAVHAGARQAWVIEEPMAAAIGA
                     GLPVEEATGSMIVDIGGGTTEVAVISLGGIVYHHSLRVGGDEMDEAIVSYARREAGLL
                     IGERTAEDVKIAIGSAYPTAEVYEVEIKGVDLVAGLPRSVVMTSNHVRDAIQEPLAAI
                     MDTVRLALENTPPELAADIMRRGIVLAGGGALLRGMDHLIMAETGIPCYVAQDPLQCV
                     VLGTARCLEAGMMNGYHGPHR"
BASE COUNT          422 a          890 c          914 g          409 t
ORIGIN      
        1 cgtgtggtcg gttgacggcg tggtcgggtc gcaaccgtgg tcggcggtga tgatgaggag
       61 gtcgtcaggc ctcaggcggg ctagcagggc ggggacggcg gcgtcgaact cctccagcga
      121 cgccgcgaag ccctcggggt cgttgcgatg gccataccgg gtgtcggtat cgttcaggtt
      181 gaccagaacc acgccggcga agtccgaggc gagggcatcc atcagtgcgg tgatcccctc
      241 ggcattgctc cccacatgcc ggcatgtgct gaaggcgcgg cccgcgaaca tgtcatcgac
      301 cttccccacg ccgcggacct caccgccggc ggccgccacg gcgtcgagct ggttgggggg
      361 cgacgggagg gcaaagtccc ggcggcgagg cgtgcggaca aaggccccgg gctcgcccgc
      421 aaaggggcgc gcgatcaccc gcgccatgtg gtccggcggc tggagcatcc cccgcgcgat
      481 ctggcaccac tggtacaact gatccacggg cacgatccct tcgtgcgccg cgatctggaa
      541 taccgaatcc gcagaggtat atacaactgg acaacccgtt ttcagatgct cgcctccgag
      601 ctcggcgatg atctcggtgc ccgaggccac ctgccctccg agcacgggcc gaccgatccg
      661 gcgggcgaac tcgtccaaca agcggccggg cagcccggcg ggataggttg ggaatggcac
      721 ggatgtgacc acacccatga gctcccagtg tccggtgatt gtgtccttac cggccgagat
      781 ctcggtcagg cgcccgtggt ggctgccggg cgccggggat cggggcaccc ccaggaccgt
      841 ggcgatgtgg cccagtccca tggccgcgag gtgcggcacg gccagtccgt cgacggcttg
      901 cgctgtgtgg gccagcgtgt gggccccctc gtcgccatag gcggcggcgt ccggcagagc
      961 tcccacgccc acgccatcga gcaccacaac gatggctcgg gcgttacgca tcgaggcgcg
     1021 tccctctgtc gggcgggccg gggcggccct tgggcgccgg ggaatggtcc ggacgcggcg
     1081 gcggctggtc tccggcctcg gcctcggctg cggcgaggtg atagcgaact tcggggtgcg
     1141 cgcccaggac gctcgccgcg gcccggagct gctccagcgc ctcttcgtac cgcccctgct
     1201 tgaagtagac ccaccccagc gaatcctgga acgtggcccg ctccagacgg aaccgctggc
     1261 tatccaggcg gcccaggaac accgagccgc cctccggacg cctcgcctca tgaagctgca
     1321 gggctcgcag cacaagctgc tcggcctggc cgagatgaac gccctgctcc gcgtacaggt
     1381 aacccagccc gttgtaagcg gtcgggtcat ctggggcgat acgcatggcg gcgcggtagt
     1441 agggctcggc gcggtggacg tccccgagca gataggcaca ctgagcggcc tggaggtaga
     1501 ggccgagaga cggggtggca tcggcgatga tggcatcgta tatggtcagg gcgtcttcgt
     1561 acatccgggc acgctcgtag gctgctgcga cccggagccc cgtctcgaaa ggcgacggtg
     1621 aggtagcgag cgccctgccg aaggcggctc cggccagccg ctggcgggtc tgcggggtga
     1681 gggattcgtc ctgcgtgatc tgcaggccct gaatgtagtg tgccgatgac tcgaaggccc
     1741 gaatgacgcg gtggccctcg tagtaccaga atgaccacag ccctcccacc agggccacta
     1801 ccaggacgta gaagcgcagg taacgcacgt gactcgatca cctctgaagc gatgcgccgt
     1861 cgatatgatg tcgttccggg acaacagcgg cacggtccgg ctggttccgg ggcctagcgg
     1921 tgggggccgt gatagccgtt catcatgccg gcttcgaggc acctggcggt tccgaggaca
     1981 acgcactgga ggggatcttg cgccacgtag caggggatac cggtctcggc catgatgagg
     2041 tggtccatcc cgcggagcag tgcgccccca cccgccagga cgataccccg ccgcatgata
     2101 tccgccgcca gttcgggcgg cgtgttctcg agcgccaggc gcacggtatc cataatggcc
     2161 gccagaggct cctggatcgc gtcgcgcacg tggttcgagg tcatgacgac actgcggggc
     2221 agtcccgcga ccaggtctac gcccttgatc tcgacctcat agacctccgc cgtcgggtag
     2281 gccgacccga tggcgatctt cacatcctcg gccgtgcgtt ccccgatcag taggcccgct
     2341 tcacgccggg catagctgac gatggcctcg tccatctcat cgccgcccac gcgcagcgag
     2401 tggtggtaca cgatgccacc cagtgaaatg acggccactt cggtggtgcc gccgccgata
     2461 tccacgatca tgctccccgt ggcctcttcg acgggcaggc ccgcgccgat ggcagccgcc
     2521 atgggttcct cgatgaccca ggcctgacgg gcgcccgcgt gcacggcggc ctccagcacg
     2581 gcgcgctgct cgacctcggt cacacccgaa ggaatcccga tcagaacccg gggat
//
LOCUS       DAOSPL010000578         2635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_38924_length_2635_cov_2.878295, whole genome shotgun sequence.
ACCESSION   DAOSPL010000578 DAOSPL010000000
VERSION     DAOSPL010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38924_length_2635_cov_2.878295"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="PLQ54_10915"
     CDS             complement(<1..1376)
                     /locus_tag="PLQ54_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93815.1"
                     /translation="MPILLRALMLTCAAGIAPAEAPAVLLPNGDFEAHSDGLPEGWAW
                     NGRDGAEVSWVADAGVGGSACIRIRTDQPGDDDAACSRELQLAPHSAYILRGKVKGTG
                     IRPGATGGDAVGAGIATSMWMCDSTRCHGANKGLGDFDWTPFALDFATGPDGKITILC
                     RLGRGGAVGEALFDDLTVEPNPDTERFEGRHFVLNLYRDEIAAATRAGVEQVIANTDL
                     AYEAYAELTGRDLGEFQLSAWGPRLWDIGALGWSGNPILWVVRRDWLEQYWARPGFCP
                     EVFLHELAHDYDYEPWVFEMHFGELFFYYACETRDLAIAEDGWRRGPAVRNRWIVRGK
                     DGVPSVCAVILKAIELRDTIGWEPFRQTYRSYLKGMEHTPIDGAHLATSADGPTGGLR
                     RELWRGSGDGELTSLTRSPRFAGPPDAVTELTRFEIPIDEEDHYGDRVSGFLCPDASG
                     EYTLWLSS"
     gene            complement(1547..>2635)
                     /locus_tag="PLQ54_10920"
     CDS             complement(1547..>2635)
                     /locus_tag="PLQ54_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93816.1"
                     /translation="LCYGSALRSGYYCALSVTVLIMAGCGDGQPGINNNGGNHNVSDA
                     LVAGPRGVQGHLTGGAVATSLRQAGVRQLVCAIGNDSGDIAGVCEPDANGDFEIALPD
                     HVQQVILAVLTDLDGDGFLDAVDAIIGFGGAVLLGIPDEDMSVDIGDVDLDEETGDVD
                     ECSDSPLDEIDTDGDGTDDWSDSDDDGDGVLDADEEEYLTGTDDDIPFIFDDDWDNDG
                     QDNPVDADDDGDGIGDPDDPCDYGISLPGWLDDDEDGIYDWQDLDDDGDGFCDGEDLD
                     DDGDGQDDWLGLDTDDDGTDDYLDADDDGDGVSDDYDSDADGDGWDDGDYGDDEDGDG
                     YPDWLNGFEEDWTDPGQGQGDEELTAAA"
BASE COUNT          472 a          943 c          781 g          439 t
ORIGIN      
        1 tcggatgaga gccacagggt gtactcgccc gaggcatcgg ggcagaggaa gccgctgacg
       61 cgatccccgt aatgatcctc ctcgtcgatg gggatctcga accgagtgag ctccgtcacc
      121 gcatcaggcg ggcccgcgaa ccgagggctt cgtgtgaggg acgtcagctc tccatcacct
      181 gatcctcgcc agagctctcg ccgcaacccg cccgtgggcc cgtcggccga tgtggcgaga
      241 tgggccccat caatgggcgt gtgctccatg cccttcagat aggagcggta cgtctggcgg
      301 aagggctccc agccgatggt gtcgcgaagc tcgatggcct tcagtatcac cgcgcagacg
      361 ctggggacgc cgtccttccc acgcacgatc cagcgattgc ggacggcagg cccgcggcgc
      421 cagccatctt cagcaatggc caggtcgcgc gtctcacagg catagtagaa gaacagctca
      481 ccgaagtgca tctcgaacac ccacggctcg tagtcgtagt cgtgggcgag ctcgtgcagg
      541 aacacctcgg ggcagaagcc aggccgcgcc cagtactgct cgagccagtc acgcctgacg
      601 acccaaagga tcgggttgcc actccacccc agagcaccaa tgtcccacag gcgtggaccc
      661 caggccgaaa gctggaactc gcccagatcg cggccggtga gctcagcata ggcctcgtaa
      721 gccagatccg tgttggcgat cacctgctcg acaccggccc gagtcgctgc cgcgatctcg
      781 tcgcggtaga ggttcagaac gaagtgccgc ccctcgaacc gttcggtgtc cgggttgggc
      841 tccacggtaa ggtcatcgaa cagggcttcg cccacagccc ctccccggcc cagtcggcac
      901 aatatggtga tcttcccgtc gggcccggtc gcgaagtcga gggcgaaggg cgtccagtcg
      961 aagtcgccca ggcccttgtt cgcgccatgg cagcgggtgg aatcgcacat ccacatgctc
     1021 gtggcaatgc cggcgccgac ggcgtcgcct cccgtggccc cgggacggat gcccgtaccc
     1081 ttgaccttgc cgcgcagaat gtaggccgag tggggcgcca gctgtagttc ccgcgaacac
     1141 gcggcatcgt catcgcccgg ctggtcggta cggatgcgga tgcaggccga cccgccgact
     1201 ccggcatccg ccacccacga gacctcggcc ccgtcccgac cgttccacgc ccaaccctcc
     1261 ggcaagccgt ccgaatgggc ctcgaaatcg ccattcggga gcagcacagc cggggcctcg
     1321 gccggcgcga tccccgcggc acacgtcagc atcagcgcgc gaagcagtat gggcatcggc
     1381 ctggtgccct cctggtagcg ttcgcaaaca gtgtgacagg ttcgcgaagc gagagccaag
     1441 ggcgttccga acaacacgga gcccggatcg gggctcggta ggcacccaac ccggactccc
     1501 tggatctcca tcgcacgggg cacggaccca cgctcggcac gtccggctag gccgccgcag
     1561 tcaactcctc gtccccctgc ccttgcccgg ggtcagtcca atcttcctcg aacccgttca
     1621 gccagtccgg gtacccgtcc ccgtcctcat catcaccata gtcgccgtcg tcccagccat
     1681 cgccgtcggc atcggagtca tagtcgtccg agacaccatc accgtcatcg tcggcgtcca
     1741 ggtagtcgtc ggtcccgtca tcatccgtgt cgaggcccag ccagtcgtcc tggccgtcgc
     1801 cgtcatcgtc caggtcctca ccgtcgcaga acccatcgcc gtcatcgtcc aggtcctgcc
     1861 agtcgtagat gccatcctca tcatcatcga gccagccggg caagctgatg ccatagtcac
     1921 aggggtcatc cgggtcccca atgccatcgc cgtcgtcatc ggcatcgacc gggttgtcct
     1981 ggccgtcgtt gtcccagtcg tcatcgaaga tgaaggggat gtcgtcgtcg gtcccggtga
     2041 gatactcctc ctcatcggcg tctaacacgc catcgccatc gtcgtcgcta tcagaccagt
     2101 catcggttcc gtcgccatcg gtgtcgatct catccagcgg gctgtcactg cactcatcga
     2161 catcgccggt ctcctcgtcg aggtccacgt cgccgatgtc cacagacatg tcctcgtcag
     2221 ggatgcccaa caacacggcg ccgccgaagc cgatgatggc atcgacggcg tccaggaacc
     2281 cgtcgccgtc caggtcggtc agcacggcca ggatgacctg ctggacatga tccggaagcg
     2341 cgatctcgaa atcgccgttg gcgtccggct cacatacgcc agcgatgtcg ccgctgtcgt
     2401 taccgatcgc gcaaaccagt tggcgcaccc cggcctgccg cagactcgtg gccaccgcac
     2461 cgccagtcag atggccctgc accccgcgag ggccggccac gagtgcatcg ctcacgttgt
     2521 gattgccacc attgttgttg atgcccggct gcccatcgcc acatccggcc atgatcaaca
     2581 cagtcactga gagagcacag tagtacccgg aacgcagagc cgacccataa cacag
//
LOCUS       DAOSPL010000579         2630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39021_length_2630_cov_3.433398, whole genome shotgun sequence.
ACCESSION   DAOSPL010000579 DAOSPL010000000
VERSION     DAOSPL010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39021_length_2630_cov_3.433398"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..1372)
                     /locus_tag="PLQ54_10925"
     CDS             complement(218..1372)
                     /locus_tag="PLQ54_10925"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOS93817.1"
                     /translation="MSRVTSAGALAVCLALCALGGCAPREFQTYAVAIGRVVVYGTDP
                     AEPVADAVVVLSGLQTRTDAEGGFRFDDIPVESMNTYLTVSRDGYRTIDEGLNLQSAG
                     DLVVALHAVADELQAGTINGQLTNSSTGEPLAQIPVTATVERLGTVVDQQTAHTSSEG
                     KFQISGIPVGETSVTATLEGYIPAKQVVTVLPGQGSNRFINLKLTPGTARVPVRGTVV
                     DVKTREPLAGVLVAVDESTDTATTDSAGAFTLSSVLVGVRTFSATLQGYDPAYVSALI
                     LAESDPIQIQMSSISEDPPPTAYNIGGNVTVEGLASAAGVTVVVTPVGASSPAARYII
                     ERGTWYGVLVPAGDYVVRASLPGFDAAEVTVSILPGEVRRDVDLTLVHGT"
     gene            complement(1369..>2630)
                     /locus_tag="PLQ54_10930"
     CDS             complement(1369..>2630)
                     /locus_tag="PLQ54_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93818.1"
                     /translation="TEATGSSILGAAIGRDDGTVSVSFNGGSSENITVSQGAFGSRVG
                     GVDSYPAKVRFTFTDADGVSTTRQVNTGYFFYVILPSILERQTLSHYFAAGTAGMHLM
                     SVPGYPVATEDSAALGVDPSQLLMAYWDPARQDELYEVYPFMPPLVPGRGYWLRVTSP
                     LQVQFEADVIPAGMSHKLYLTPGWNLVGCPFTQNVNVADLAVDNGSGSVSFAQAVQNA
                     WVRGLVFGYTEAAGYQEASSLVPWSGYWMYCKVSGVTVTVPNPTAAASSVRAGVRSVP
                     EPEWAVRLRAEPPSGVTASVLLGAARGASEGFDPSFDVEALPPGPGQPLIVSALADGG
                     PECGLAREVRPVKGGVVTYTIRVGAVDGSLPAVLCAENVPPGWRLGICDATTGAALPS
                     RGPGRFGPLAVTPSPRLLWVTLESVGS"
BASE COUNT          465 a          886 c          902 g          377 t
ORIGIN      
        1 atgtcctgcg cccgagccgc gccatagtat tcacccgaag ccgagcgaga cctgtcgcca
       61 ccgtcaggca ccggcagacg atccagagat ccgtgtctcg caccgaatct aacgaacacg
      121 ggcgccggac cgtctcggga atcgacaccc gcagcggccc cgttgtccca atgggccggg
      181 agacatgccg gttcatcgcc agaccacggc cgcgatctca agtgccatgc accagagtca
      241 gatcgacgtc gcggcggacc tcgccgggga ggatgctgac agtgacctcc gcggcgtcga
      301 acccggggag actggcgcgg accacatagt cgcccgcagg cacgaggaca ccgtaccagg
      361 tgcccctctc aatgatatag cgcgcggcgg gcgagctggc gcccacgggc gtcaccacca
      421 cggtcacacc ggccgccgaa gccaagccct cgacggtgac attgccgccg atgttgtagg
      481 ccgtcggggg cgggtcctcg ctgatggatg acatctggat ctggatggga tcgctttcgg
      541 ccaggatcag agccgacacg taggccgggt cgtagccctg gagcgtggcc gagaaggtgc
      601 gcacgccgac cagcacgctc gagagtgtga aggctccggc cgagtcggtg gtggcggtgt
      661 ctgtcgactc atcgacggcg accaggaccc cggccagggg ctcgcgcgtc ttgacgtcga
      721 cgacggtccc tctcaccgga actcgcgccg tcccgggcgt gagttttaga ttgatgaacc
      781 gattagagcc ctggcccggc agtaccgtga cgacctgctt ggcgggaatg tagccttcga
      841 gcgtcgccgt gacgctggtc tcgcccacgg ggatcccgct gatctggaac ttgccctcac
      901 tggatgtgtg agccgtctgc tgatcgacca cggtgcccag gcgctcgacc gtggcggtga
      961 ccgggatctg agcgaggggc tcgccggtgc tcgagttggt gagctgcccg ttgatggtgc
     1021 cggcctgcag ctcgtcagcc accgcatgga gggcgacgac caggtctccc gcagactgca
     1081 ggttcaggcc ctcgtcgatg gtgcggtagc catcgcgcga gaccgtgagg taggtgttca
     1141 tgctctcgac ggggatgtcg tcgaaccgga agccgccctc ggcgtcggtc cgcgtctgaa
     1201 gccccgagag cacgaccacc gcatcggcca ccggttcggc cgggtcggta ccgtatacga
     1261 cgacccgacc gatggccacc gcataggtct ggaactctcg cggcgcacag ccccccagcg
     1321 cgcacagggc cagacagacc gccagcgctc cagcggaggt cacccggctc atgagcccac
     1381 cgactcgagg gtaacccaga gcaaccgggg actcggtgtg accgccaacg gcccgaaccg
     1441 gcccgggcct cgggacggca gggcggcccc tgtcgtggca tcacagatgc ccagccgcca
     1501 gccgggcggg acgttctcgg cgcagaggac cgccggcagg gagccgtcga ccgccccgac
     1561 ccggatcgtg taggtcacga ctccaccctt cacgggacga acctcgcgcg ccagcccaca
     1621 ctcggggccg ccgtcggcca gggcggacac gatgagtggc tgtccgggcc cggggggcag
     1681 agcctcgaca tcgaaggagg gatcgaagcc ctcgctggca ccgcgtgccg cgcccaacag
     1741 cacggaagcg gtcactccgg aagggggctc ggcccgcagc cgcacggccc attcgggctc
     1801 cggcaccgag cgaacgcccg cgcgcaccga agacgcggcg gcagtcgggt tcggcaccgt
     1861 gaccgtcacg ccgctgacct tgcagtacat ccagtagccg gaccacggga ccaggctgct
     1921 cgcttcctgg tagccggcgg cctcggtgta gccgaacacc aggccgcgca cccaggcgtt
     1981 ctgcacggct tgggcgaagc tgaccgagcc cgagccattg tcgacggcca ggtcggcgac
     2041 gttgacgttc tgggtgaacg gacagccgac caggttccac cccggtgtga ggtagagctt
     2101 gtgggacatt cccgccggga tgacatcggc ctcgaactgg acctgcaggg gcgaggtcac
     2161 ccgcaaccaa taaccccggc cggggacgag aggcggcatg aacgggtaga cctcgtagag
     2221 ctcatcctgc cgggctggat cccagtaggc catgaggagt tgcgaggggt ccacgccaag
     2281 cgcggctgag tcctcggtgg ccacgggata gccgggcacg gacatgaggt gcatgcccgc
     2341 ggtacccgcc gcgaagtagt gggaaagagt ctggcgctcc aggatgctcg gcaagatgac
     2401 atagaaaaag tagccggtat tcacctgacg agtcgtggag acgccatcgg cgtcggtgaa
     2461 ggtgaagcgc accttggcgg gataggagtc gacgccgcca actcgcgatc cgaaggcccc
     2521 ctgggacaca gtgatattct cggacgatcc cccgttgaag gacacgctca cggtgccgtc
     2581 atcccgcccg atcgctgccc cgagtatcga gctgccggtg gcctcggtct
//
LOCUS       DAOSPL010000580         2628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39054_length_2628_cov_3.019044, whole genome shotgun sequence.
ACCESSION   DAOSPL010000580 DAOSPL010000000
VERSION     DAOSPL010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39054_length_2628_cov_3.019044"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PLQ54_10935"
     CDS             <1..591
                     /locus_tag="PLQ54_10935"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOS93819.1"
                     /translation="DIRADQGDVVCHVATDNVEGGRLAGERMAELLKGKGEVIIIDHP
                     QVASVQDRTRGFVEALAKHPGIKVVARPVGAGVRETARKAMEDALIAHPEMDGVFCIN
                     DSTAMGALAALQSQVKPPNIVMVSYDGDPEACEEIRRGTALKADVVQYPAKIGSQVIQ
                     AIADHLSGKPVPKEMPVKPGIVDAESLAATHGKPAE"
     gene            608..1618
                     /locus_tag="PLQ54_10940"
     CDS             608..1618
                     /locus_tag="PLQ54_10940"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS93820.1"
                     /translation="MDREQRRHALTLAASRYGMVVFLVALCAYFGLATREHRFLSVDN
                     LLNVANQVSINAVLALGMTFVIISAGIDLSVGSVAALVGVLTAGTMTRGLGFHIGGYE
                     IVLVPRLDPGAMIAFGVLVGLLIGAVMGALNGLFVVRFRVPPFIITLAMMLIARNLAL
                     IYDDQPVAPLPRTFEWIGMWGRIAGVPIPVFITAVLALVAWVVLSRTVYGRQVYAVGG
                     SEEAAHVSGVRVNRVLLGVYVICGLAAAIAGVLETSQSGAGDPNAGELWELNAIAAVV
                     LGGTSLMGGRGTIPGTLLGALIIGVLSNGLRLMDVAPFSQGVVKGCVILFAVVLDRLT
                     SE"
     gene            complement(1763..>2628)
                     /locus_tag="PLQ54_10945"
     CDS             complement(1763..>2628)
                     /locus_tag="PLQ54_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93821.1"
                     /translation="AWEILNEPIYTDYSLPKSEGYTVSDYTRYLRAASEAVKRADPNA
                     LVIGGIAGPPDLMTAEFIAEGGLEHIDALCLHAYPGLTPPETFDEPLSQLVERMEAAG
                     HRRPIWFTEGAYYADDDPAETPWESWLSLVDDEALCGAWMTKLSAMLLAYGTERIVWH
                     SGTPGALNRDEPAGIFFEWEGAPRKMLVSQAAMSEIVGPGPLHGRGRLASPESLYAYA
                     FDSGERTIVVAWLNEDMTETPVRLALQGARAFDLVGNRLYGDAVTVGFEPVYVVFERV
                     AGDAELSRLAG"
BASE COUNT          406 a          914 c          862 g          446 t
ORIGIN      
        1 gacatccggg ccgatcaggg cgatgtggtt tgccatgtgg ccaccgacaa tgtcgaaggc
       61 ggccgtctcg cgggcgagcg tatggccgaa ctcctcaaag gaaagggcga agtcatcatt
      121 atcgaccacc cgcaggtcgc atcggtgcag gaccgaacca gaggcttcgt cgaggccctg
      181 gccaagcacc ccggtatcaa ggtcgtcgcc cggcctgtgg gggccggcgt ccgcgagacg
      241 gcgcgcaagg ccatggaaga tgctctcatc gcgcaccccg agatggatgg cgtgttctgt
      301 atcaatgact ccactgccat gggcgccctc gcagctctgc agtcacaggt gaagccaccc
      361 aacatcgtca tggtgagcta tgatggcgac ccggaggcct gtgaggagat ccgccgcggc
      421 acggcgctga aagccgacgt ggtgcagtac ccggcgaaga ttggcagcca ggtcatccag
      481 gccatcgccg accacctgag cgggaagccg gttccgaagg aaatgcccgt caagccaggc
      541 atcgttgacg ccgagagcct cgccgccacg cacgggaagc cggccgagta gagaggctcg
      601 aactctcatg gaccgcgagc agaggcgcca cgcgctcacc ttggcggcgt cgcgctatgg
      661 catggtggtg ttccttgtcg ccctgtgtgc ctacttcggt ctggccacgc gcgagcatcg
      721 cttcctgagc gtcgacaacc tgctcaacgt cgccaaccag gtctccatca acgccgtcct
      781 cgcgctcgga atgaccttcg tgatcatctc cgccgggatc gacctctcgg tcgggagcgt
      841 cgcggctctg gtgggtgtcc tcaccgccgg caccatgacc cgtggtctcg gctttcacat
      901 cggcgggtac gagatcgtcc tcgtgccccg gctggacccg ggagcgatga tcgccttcgg
      961 cgtactcgtc ggcctcctca tcggcgcggt gatgggcgcg ctgaacggcc tctttgtggt
     1021 gcgcttccgc gtgccaccat tcatcatcac cttagccatg atgctcattg cgcggaacct
     1081 ggctctcatc tatgacgacc agccggtggc gccactgcct cggacgtttg agtggatcgg
     1141 catgtggggc cgcatcgccg gtgtgcccat ccccgtgttc atcaccgccg tgctggcgct
     1201 cgtcgcctgg gtagtgctct cccggaccgt ctacggccgc caggtctacg ccgtgggcgg
     1261 cagcgaggag gccgcacacg tctctggcgt gcgcgtgaac cgcgtgctgt tgggcgtgta
     1321 cgtgatctgc ggcctcgccg ctgccatcgc gggggtcctc gagacctcgc aaagtggcgc
     1381 cggcgacccc aatgccggtg agttgtggga gctcaacgcg atcgctgccg tcgtcctcgg
     1441 tggcaccagc ctcatgggcg gccggggtac catacccggc acgctgctgg gcgcactcat
     1501 catcggtgtc ctcagcaacg gcttgcgcct gatggacgtc gcgcccttct cgcagggcgt
     1561 cgtcaaaggc tgcgtcatcc tcttcgccgt cgtgctcgat cgcctcacgt ccgagtaggc
     1621 agccgcgccc gcaggagccc tggtgactcg ggcggcaggc ggatggggtc caggggacgg
     1681 ctcccctggc ggggtgcggg gcggagcccc gtgatgtccg accgcgggcg aaacgcgggc
     1741 gggcggcctg ccagccgtga tcctagcccg ctagcctcga cagctccgcg tcgccggcca
     1801 cgcgctcaaa caccacgtag accggctcga acccgaccgt caccgcgtcg ccatacagcc
     1861 ggttgccaac caggtcgaac gcccgagccc cctgcagggc gagccggact ggcgtctcgg
     1921 tcatgtcctc gttgagccac gccacgacga tcgtccgctc gccgctgtcg aaggcgtacg
     1981 cgtacaggct ctccggcgag gccagacgcc cgcgaccgtg caggggaccg gggcccacga
     2041 tctccgacat ggccgcctgg gagaccagca tcttccgtgg cgcgccctcc cactcaaaga
     2101 agatccccgc tggctcgtcc cggttgagcg cacccggcgt ccccgagtgc cagacgatcc
     2161 gctccgtgcc gtaggccagc agcatcgcgc tgagcttggt catccaggcg ccgcacagcg
     2221 cttcgtcatc cacgagcgac agccaggact cccacggcgt ctccgccggg tcgtcatcgg
     2281 cgtagtaggc cccctcggtg aaccagatgg gccgtcggtg tccggctgcc tccatgcgct
     2341 cgacgagctg cgacagcggc tcgtcgaagg tctccggcgg cgtcaggccg gggtacgcat
     2401 ggagacagag ggcgtcgatg tgctcgagcc cgccctccgc gatgaactcc gcagtcatca
     2461 ggtccggcgg gccggcgata cctccgatga ccagcgcatt cgggtccgcg cgcttcaccg
     2521 cctcagaagc cgctctcagg taacgcgtgt agtccgacac ggtgtagccc tcggacttcg
     2581 gcaggctgta atccgtgtag atgggctcgt tcaggatctc ccacgcgt
//
LOCUS       DAOSPL010000581         2626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39084_length_2626_cov_3.880980, whole genome shotgun sequence.
ACCESSION   DAOSPL010000581 DAOSPL010000000
VERSION     DAOSPL010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39084_length_2626_cov_3.880980"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="PLQ54_10950"
     CDS             complement(<1..1263)
                     /locus_tag="PLQ54_10950"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOS93822.1"
                     /translation="MRSPIALTVLVALAAAGAVGAAPATALDRQRHLEWFDDARFGMF
                     VHWGIYSVIGMEASWPLYGKQFTREEYEGQARQFNPADFRADELVGLAKRAGMKYLVI
                     TTKHHDGFCMYDSKLTDYTIMNGPCKRDLIGEVADACHRHGLRLGFYYSLCDWHDPRY
                     LSMPVQGGPFPLGDIRRDAHTWREFTRFVHGQVRELLTNYGRVDIMWFDGGWEHTPEE
                     WRSEELHQMIRTLQPHILINNRLPGEGIADYVTPEQMIPSSAPSGPWETCMTINNTWG
                     YNPRDREFKPAELLIRNLVQTASGGGNYLLNVGPGPDGSIQPEFRERLLAVGDWLRDH
                     GEAIYGTQRGPRRLSDQATATTKRNALYLHMADPPKDYLVLDRLASPVLGAWMVPSRK
                     PVRVQRLGRGARLVLDPTQFDPVSTVIEL"
     gene            complement(1475..>2626)
                     /locus_tag="PLQ54_10955"
     CDS             complement(1475..>2626)
                     /locus_tag="PLQ54_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOS93823.1"
                     /translation="KARAHHAAEEAARLQGILDHLAEPFFTVDRDLTITYMNEACARA
                     VGYEPAEAVGRLKCKDVFRSDICETNCAVQRCMRTGEAISGARVTIRNRRGDAIPILA
                     TVSTLTDAAGNVVGGYELVRDFTAEALVEGAVREATEQITSATGQVARGITETTQAAE
                     QISVGVQGIAEEMASVSSAASLGTEAAVRGSQAALATVHSMDSVSGAAAEIVEGMRAL
                     NVKAEEIGRIIEVIDGVADQTNLLALNAAIEAARAGEHGKGFAVVADEVRKLAERAAD
                     ATREVNDLLQGIRQLVEQRTHDVETTARAIEESVCQVTDTAQVFDEVAEAIRQTHSSA
                     QSVAATVEQLSASAEEMSASFEEIAASAQELAGMAESLDAAAARLQGAG"
BASE COUNT          401 a          855 c          909 g          461 t
ORIGIN      
        1 gagctcgatg acggtggaga cagggtcgaa ctgcgtgggg tcgaggacaa gccgggcgcc
       61 ccggccgagc cgctggacgc gcaccggctt gcggcttggg accatccacg cgccgaggac
      121 gggagaggcg agacggtcga gtacgaggta gtccttgggc gggtcggcca tatgcaagta
      181 gagggcgttg cgcttggttg tggccgtggc ctgatccgag aggcggcgcg gccctcgctg
      241 ggtcccgtag atggcctcgc cgtggtcccg aagccagtcg cccacggcca ggagacgctc
      301 tctgaactcg ggctggatag acccgtctgg gcccggccct acgttcaaca ggtagttccc
      361 cccgcccgag gccgtctgga ccaggttccg gatgagcagc tcggcgggct tgaactcgcg
      421 atccctcggg ttgtagcccc aggtgttgtt gatggtcatg caggtctccc acggccccga
      481 cggcgcggac gacgggatca tctgctcggg ggtcacgtag tcggcaatgc cctcgccagg
      541 gagccggttg ttgatgagga tgtgaggctg gagggtgcgg atcatctggt ggagctcctc
      601 ggaccgccac tcctcgggcg tgtgctccca cccgccatcg aaccacatga tgtcgacgcg
      661 gccgtagttg gtgagaagct cgcgcacctg gccgtggacg aagcgggtga actcgcgcca
      721 tgtgtgggca tcgcgccgga tgtctccgag tgggaacgga ccgccctgga cgggcatgga
      781 caggtagcgc gggtcgtgcc agtcgcagag cgagtagtag aaaccgaggc gaagcccgtg
      841 ccggtggcag gcgtcggcca cctcgccgat caggtctcgc ttgcaggggc cgttcatgat
      901 cgtgtagtcc gttagcttcg agtcgtacat gcagaagccg tcgtggtgct tggtcgtgat
      961 caccaggtac ttcatgccgg ccctcttggc caggccgacg agctcgtcgg cccggaagtc
     1021 ggccgggttg aactgcctcg cttgaccctc gtactcctcg cgggtgaact gcttgccata
     1081 gagcggccag gatgcttcca tgccgatcac cgagtagata ccccagtgga caaacatgcc
     1141 gaaccgggca tcatcgaacc actcgaggtg gcgctggcgg tcgagggcgg tggcgggtgc
     1201 cgcgccgaca gcaccggcgg cggcaagggc gacgagaaca gtcagggcga taggcgagcg
     1261 catggtgggc ctccgggacc acttgggcaa tgcgcgtcag ttcgccccag ttcgccggca
     1321 ggcctgccga ttggggtggg gctgaaagaa gaacggcgcc ggctggcgca ccgccggggg
     1381 ggctgagtgc ggccgttgca ggaccggcgc cgatcacaat ctctatcgtg ccctcggggc
     1441 gaaacgttgc acccgattct cgacgcggcg gatcctagcc agcgccctgc agtcgcgcgg
     1501 ctgcggcgtc gagcgactcc gccatgccgg cgagttcctg ggccgaggcg gcgatctcct
     1561 caaacgatgc gctcatctcc tcggcggatg ccgagagttg ctccacggtg gctgcgacgc
     1621 tctgcgcgct ggagtgggtc tggcgaatgg cctcagccac ttcatcgaac acctgggcag
     1681 tgtccgtgac ctggcagacg ctctcctcga tggctcgcgc cgtggtctcg acgtcgtggg
     1741 tgcgctgctc cacgagctgg cggatgcctt gcaggaggtc gttcacttca cgcgtggcat
     1801 cggccgcgcg ttccgcgagc ttcctcacct cgtccgcgac cactgcgaag cccttaccat
     1861 gctcgccggc ccgggcggcc tcgatggcgg cgttgagggc gagcaagttg gtctgatctg
     1921 ccaccccatc gatgacctcg atgatccggc cgatctcttc agctttcacg ttcagggccc
     1981 tcataccctc aacgatctca gcggccgcgc cgctcacgct gtccatggag tggacggtgg
     2041 cgagcgccgc ctggctcccg cgtaccgcgg cctccgtccc gagcgaagcc gcggaggaca
     2101 cgctcgccat ctcctcggcg atgccctgca cgccaaccga tatctgctcg gccgcctggg
     2161 tcgtctccgt gatgccgcgg gccacctgtc cggtggcact ggtgatctgt tcggtggcct
     2221 cacggacagc gccctccaca agagcctcag ctgtgaagtc ccgaactagt tcgtagcccc
     2281 cgacgacatt gcccgccgcg tcggtgagag tcgagacggt ggcgaggatg ggtatggcat
     2341 cgccccggcg attccggatc gtgacacgcg cgccggaaat ggcctccccc gtcctcatgc
     2401 aacgttgcac ggcgcagttg gtctcgcaga tgtcgctccg gaacacgtcc ttacacttca
     2461 gccggcccac ggcttccgcg ggctcatagc ctacggcccg agcgcaggcc tcattcatgt
     2521 acgtgatggt cagatctctg tcgaccgtga agaacggctc cgccaggtgg tcgaggatgc
     2581 cctggagccg ggccgcctcc tcagcggcat ggtgggcgcg ggcctt
//
LOCUS       DAOSPL010000582         2626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39086_length_2626_cov_3.070790, whole genome shotgun sequence.
ACCESSION   DAOSPL010000582 DAOSPL010000000
VERSION     DAOSPL010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39086_length_2626_cov_3.070790"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="PLQ54_10960"
     CDS             complement(<1..1111)
                     /locus_tag="PLQ54_10960"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOS93824.1"
                     /translation="MRLSCVAVQPVARAGETLITQALLTIQAPPRSAGAAMPTALALV
                     LDRSGSMDGPAFDRARAAMRAAMDTMRPEDYLCVVASGRRPELLIPARPVASRTGFRA
                     LTNSVSCQDWGPVSSALECAVETLQQVPSEVWPRRALLLTDGWLGAPEHARLRRAAEL
                     AASVGVRLDVVLCGSEGDLLALYELARLTLGACRCVNSTEEAHRTGHRWAEDLTQVAA
                     SQLELTVASTEGCELTPMAPCRGTRLPDLALGSQTGVGLRLRCDPRAPGVYRMGQVEL
                     AYRNASTGIPAVARADIIMEFADHGAQGLEDADDPEVARHLLLDAELESLGQVLSEWR
                     PDRKSAGSAASVLRERAGALQAAGLTETAAMVWKAE"
     gene            1276..1686
                     /locus_tag="PLQ54_10965"
     CDS             1276..1686
                     /locus_tag="PLQ54_10965"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS93825.1"
                     /translation="MRIPSRPEFAVRVREEAFRRALQAGFPTPEAEDVALAVHEAVIN
                     AIEHGNQSDPTRVVAVTFVDDSSALVIEVRDEGTARDSEPSAGGPLPSAGAPRGRGIP
                     LMRALMDDVQLFPERGRVTLRRRKPSSAGATDGG"
     gene            1691..2464
                     /locus_tag="PLQ54_10970"
     CDS             1691..2464
                     /locus_tag="PLQ54_10970"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOS93826.1"
                     /translation="MLLAIDVGNTSTMMGLFDGAKLVRDWRIGTHSDGTSDEYGLRIV
                     GLLRESGIERREVSAVVMASVVPRATSRLGESCTRYLGRSPLIVGPECGGVATEYVPP
                     DAVGPDRLANAVGAGALYGWPAIVVDFGTTTTVDAVDAEGTYLGGAIAPGVEVSVQAL
                     YACAARLSWVEMEPPRRAIGRSTAESLRSGVIYGFAGQVDGLVRRFLAELGAEAHVIA
                     TGGQAGLIVPWTETVARVDEHLTLHGLRVIWERESRRGD"
BASE COUNT          359 a          843 c          970 g          454 t
ORIGIN      
        1 cctcggcctt ccacaccatg gcggccgttt cggtgagccc ggccgcctgg agggccccgg
       61 cgcgttctcg gagcaccgag gcggcgcttc ccgccgactt ccggtcggga cgccattcgg
      121 agagcacctg gcccagactc tccagttcgg cgtccagcaa caagtgtcgt gccacctcgg
      181 gatcgtcggc gtcctccagg ccctgggcgc cgtggtccgc gaactccatg atgatgtcag
      241 ctcgcgccac agcagggatc cccgtcgagg cgttccggta cgccagctcg acttgcccca
      301 ttcggtatac cccgggtgcc ctggggtcgc accgaagccg gagtcccaca ccggtttggc
      361 tgcccagagc caggtccggt aaccgggtgc cgcgacacgg agccattggc gtcagctcgc
      421 atccctccgt ggaggcgacg gtgagctcca gctggctcgc cgccacctgt gtgaggtcct
      481 cggcccaccg gtgtcccgtg cggtgagctt cctctgtact attgacgcat cggcaggctc
      541 cgagggtcag tcgtgcgagt tcgtagagag ccagcagatc gccctcgctg ccacatagta
      601 caacgtccag ccgcacgcca accgatgcag cgagttcggc ggctcgacgc aggcgcgcgt
      661 gttccggggc cccgagccac ccgtcagtca gcagcagcgc tcgtcgcggc cagacctcag
      721 atgggacttg ctggagcgtt tcgacggcac actcgagggc ggaactgacg ggcccccaat
      781 cctggcatga caccgagttg gtcagcgcgc ggaatccggt tcggctggcc acggggcgcg
      841 ccgggatgag cagctcgggg cggcgtcccg aagcgaccac acacaggtag tcctcggggc
      901 gcatggtatc catggccgcc cgcatggcgg cacgggcccg atcgaaggca gggccgtcca
      961 tggacccgct acggtcgagc accagggcca aagctgtggg catggcggcg ccggcgctgc
     1021 gaggcggggc ctggatggtc aggagcgcct gggtgatgag agtctctccg gcccgggcca
     1081 cgggttggac ggcgacacat gataggcgca attggcaacc gcctccgacg cgggtggctg
     1141 tgggcccccg gggtgcatgt tcgggccctg gggtcgtcga gcctgcagga gctcgggccg
     1201 gtccggagga accgggacgg cgccagttcg ggctgcgaag ctgaccgcgt ccagcccgcc
     1261 tgggttgccc acaccatgag gatacccagt cgaccggagt tcgccgtgcg cgtgcgcgag
     1321 gaggccttcc ggcgcgccct gcaagccggg ttcccgaccc cagaggccga ggacgtggct
     1381 ttggccgtgc atgaggcggt cattaatgcc atcgagcatg gtaaccaatc ggatcccacc
     1441 cgggtggtgg cggtgacctt tgttgatgac tcctcggccc tggtgatcga ggtgcgcgat
     1501 gagggaacag cgagggactc tgagccgtcg gcgggcggac ctctgccgtc ggctggcgcg
     1561 cctcgcggcc gagggattcc actgatgcgc gcgctcatgg atgacgtcca gctcttcccg
     1621 gagcgtggtc gagtgactct ccggcgccgc aagccgtcgt ccgccggcgc cacggacggg
     1681 ggttaggggt ttgctgctgg ccatcgatgt agggaatacc tcgacgatga tgggcctgtt
     1741 tgacggggcg aagcttgtcc gggactggcg gatcggcacc cattcggacg gtacttcgga
     1801 cgagtatggc ctccgcattg tgggtctgct cagggagtcg ggcattgagc ggcgcgaggt
     1861 gagtgccgtg gtcatggcga gtgtggtgcc gagggccaca tcgcgcctcg gggagtcgtg
     1921 cacccggtat ttggggcgct ctcctctgat cgtcggcccg gagtgtgggg gagttgccac
     1981 cgagtatgtc ccccctgatg ccgtgggtcc cgatcggctc gcgaatgcgg tgggggcggg
     2041 ggcgctctat ggctggcctg ccatcgtggt cgatttcggc accaccacca cggtggacgc
     2101 cgtcgacgcc gaagggacgt acctgggtgg ggccatcgct ccgggtgtcg aggtttcggt
     2161 acaggcgctc tatgcctgtg ccgcccgcct gtcgtgggtt gagatggagc ctccccggcg
     2221 cgccatcggc cggtcgacgg cggagagtct gagatccggc gtgatctacg ggttcgccgg
     2281 gcaggtcgat ggtcttgtgc gccggttcct ggccgagctg ggagctgagg cccatgtcat
     2341 tgccacgggg ggccaggcgg gcctcatcgt cccctggacc gagacggttg cccgtgtgga
     2401 cgagcacctg accctgcatg ggctgcgggt gatctgggag cgagagtcgc ggcgtgggga
     2461 ctgatcggcc gccggaccaa gccggcgtgc tcgagcgcgt cgctggcggg cgttggtgtt
     2521 gggccctcct gggggcggcg ggagtcgtgc cctacgtggt gtcactgcgc ggcacgttcg
     2581 tctccgatga cctgctgttc gtccgggatt ccttcggcct ggcgcg
//
LOCUS       DAOSPL010000583         2625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39105_length_2625_cov_3.045136, whole genome shotgun sequence.
ACCESSION   DAOSPL010000583 DAOSPL010000000
VERSION     DAOSPL010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39105_length_2625_cov_3.045136"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1781
                     /locus_tag="PLQ54_10975"
     CDS             174..1781
                     /locus_tag="PLQ54_10975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOS93827.1"
                     /translation="MCATACVFAVAFGSVSLTSADMGLTYDEPIYGSKAMLAREWLAL
                     LAGGQASYAVSNEAIGHYWAGKDQHPGVVKLVGALGSATIGSALGGLASFRVAGPLFF
                     ATLCASVCWFLQRYGWPAAVFGTGSLATMPRVFGHAHLMALDVPVAAMSFLTAVAFLK
                     AVRDPRWRWAVVFAVVWGLALGCKLNAFFVPFPLVAWWLVYRGGRPLRLLACAALVAP
                     LVFWLTWPWLWYDTIARVGSYVAFHGGHGRIAVDYLGRTYADPPAPWHYPLVMTAITT
                     PGVTLLAWCYGLWNHVGRADRRRPEAVLMAMCALVQMAAVALPGVPKYNGVRLFLPAF
                     PFLCVLGGLGAATALDGLARWGEGRGGLGGIVGRRGLVTALAPVLLLMPGLAATVGSF
                     PSWLSYYNGLVGGPRGALRAGFEPTYWGDAYLRPLAWLSSNAPSRARVVICQPGIIGT
                     LVDSYRGAVVREDLEFTEDPRELRSADYCVFHTRRNEVWPQARVLLDRGRPVYVWDLD
                     GAPLAVVYEAAEVRRVLSEGNAEPAAN"
     gene            1823..>2625
                     /locus_tag="PLQ54_10980"
     CDS             1823..>2625
                     /locus_tag="PLQ54_10980"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOS93828.1"
                     /translation="MAEQCAPTDADAVAAKLALRERQLYAIARISAALYSQVRLDTLL
                     PEIINVSLAVAGADAGSILLYDPKTDDLEFRYVVGEASERLTGEHHSVSTGICGRVFS
                     TGVAEISDDPRSDESHDAEVGQRVSYVTRNMVTVPLKTGEGEVIGVMQILNKHEGLFD
                     EADLEVLSILANQAAMAIEHVELMRRVRVAETVKKIGDISHDIKNMMTPVESGMKTLE
                     MIYHSAFEAYEAVRDEARELDGSAVFCRLDEAFASLSEFFPEITLMVLEG"
BASE COUNT          363 a          850 c          929 g          483 t
ORIGIN      
        1 cgcgcttcgt cgccagcgag ctcgacaagc gtgcccgggt gctcatgcgg ctgcactcgg
       61 tgctccagcg ggtgccggcc gggccggatg ccgcgaagcc gacgcactga gaaggctccg
      121 aagtgtggga agggcaaacg cacgagcctc ggccacgccg cagttgggtg ccggtgtgcg
      181 cgactgcctg tgttttcgcg gtcgccttcg gatcggtgtc gctgacgtcg gccgacatgg
      241 gtctaaccta cgatgagccg atctacggat ccaaggccat gctcgcgcgc gagtggcttg
      301 ccctcctcgc tggcgggcag gcgtcctacg ccgtctcgaa cgaggccatc ggacactact
      361 gggcggggaa ggaccagcac ccgggtgtcg tgaagcttgt gggcgccctc gggtccgcca
      421 ccatcggcag cgcgctgggc ggcttggcgt cgttccgggt ggcggggcct ctattcttcg
      481 cgaccctctg tgcgtctgtg tgctggttcc tgcagaggta tgggtggccc gcggcggtat
      541 tcggcaccgg tagcctcgcg accatgccgc gagtgtttgg gcacgcccat ctgatggctc
      601 tggacgtgcc ggtagcggcg atgagtttcc tgaccgccgt cgccttcctc aaagcggtcc
      661 gcgacccgcg gtggaggtgg gcggtggtct tcgccgtggt ctggggtctg gccctgggct
      721 gcaagctgaa tgcgtttttc gtccccttcc cgctggtcgc ctggtggctg gtctaccgcg
      781 gcggcaggcc gttgcggctg cttgcctgcg ccgctctcgt cgcgccattg gtgttctggc
      841 tcacgtggcc atggctctgg tatgacacca tcgcccgggt cggcagctat gtggccttcc
      901 acggtgggca cggccgtatc gccgttgact acctcggacg gacctacgcc gatccgccgg
      961 cgccgtggca ctatccgctg gtgatgaccg ccatcaccac gcccggggtg accctgctgg
     1021 catggtgcta cggcctgtgg aaccacgtcg gtcgtgccga ccgccgacgg cccgaggcgg
     1081 tcctcatggc gatgtgcgca ctggtccaga tggctgcggt cgcgctcccc ggcgtaccca
     1141 agtacaacgg cgtacggctg ttcctccccg cgttcccgtt cctgtgcgtt ctgggagggc
     1201 ttggcgcggc gacggctctc gatggtctgg cccggtgggg agagggccgc ggcggtcttg
     1261 ggggtatcgt gggacgccgc ggcctggtga ccgcgctggc gccggttctg ctcctcatgc
     1321 cagggcttgc cgccaccgtc ggctcattcc cgtcctggct ttcgtactac aacgggctgg
     1381 tcggcggccc gcgcggggcc cttcgggccg gcttcgagcc gacgtactgg ggcgatgcgt
     1441 acctgcgtcc gctggcatgg ctctcttcga atgcgccctc tcgtgctcga gtggtcatct
     1501 gccagccggg catcatcggc accctggtgg actcctaccg gggggccgtt gtgcgtgagg
     1561 accttgagtt caccgaggat ccccgggagc tgcgatcggc ggactactgc gtcttccaca
     1621 cccggcgcaa cgaggtctgg ccccaggcac gtgtgctcct cgatcggggg cgccccgtct
     1681 acgtgtggga cctcgatggc gccccactgg ccgtcgtcta cgaggccgct gaggtgcggc
     1741 gcgtgctgag tgaggggaat gcggaacccg cggcgaacta ggcccgggcg actcgcacga
     1801 tcgcggagag gcagtgggcg ggatggcaga gcagtgtgcc ccgacggacg ccgatgcggt
     1861 ggcagcgaag ctggcactac gggaacggca gctctatgcc atcgcgcgta tctcggccgc
     1921 gctgtactcg caggtgcgcc tggacacgct gctgccggag atcatcaacg tctccctggc
     1981 ggtggccgga gccgacgcgg gttcgatcct cctctatgac cccaagaccg acgaccttga
     2041 gttccgctac gtggtcggcg aggcgagcga gcgcctcacc ggggagcatc actcggtgtc
     2101 cacgggcatc tgcgggcggg tcttctcgac gggcgtggcc gagatcagcg acgacccgcg
     2161 atcagacgag tcgcacgatg ccgaagtcgg ccagcgcgtc tcgtatgtga ctcgcaacat
     2221 ggtgaccgtg cccctgaaga ccggcgaggg cgaggtcatc ggagtgatgc agatcctgaa
     2281 caagcacgag gggctgttcg acgaggcgga cctcgaggtg ctgtccatcc tggccaacca
     2341 ggccgccatg gccatcgaac acgtcgagct gatgcgccgt gtccgcgtcg ccgagactgt
     2401 gaagaagatc ggggacatca gccacgacat caagaacatg atgacgccgg tcgagagcgg
     2461 catgaagact ctcgagatga tctaccacag cgcgttcgag gcctacgagg ccgtgcgaga
     2521 cgaggcgcgg gaactcgatg gcagtgccgt cttctgccgg ctcgatgagg cattcgcctc
     2581 actgtccgag ttcttccccg agatcacgct gatggttctc gaggg
//
LOCUS       DAOSPL010000584         2621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39178_length_2621_cov_3.486360, whole genome shotgun sequence.
ACCESSION   DAOSPL010000584 DAOSPL010000000
VERSION     DAOSPL010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39178_length_2621_cov_3.486360"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1968
                     /locus_tag="PLQ54_10985"
     CDS             <1..1968
                     /locus_tag="PLQ54_10985"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOS93829.1"
                     /translation="IDATLDMAEPYHNFTAVIATGLCVGAIALHGEDARADAWISHAI
                     ARAKLNFDAQGKDGGAMEGPMYGTYAASMFADMIWALTTAGVENDLAAHPYIESMPRY
                     CISLLDPGTRRQPCFGDGGPTAGFGELMLILALRGSADAAWYCQQIGQLDAKDVRRFL
                     ALDPARIRPAEPTWSPSGCFVDVGYAILRDGYSRDSAFMALKCGPPEADVGHNHFDHN
                     SFVISFAGAWPAWDPGYRSYFDPPKRRYTTSTLGHNTIVVDLDDAYLASQEAAQPGHD
                     QLHVNRGRITEFYAGPGFDYALGDAANAYNTDAAHLLDAFTRQIVFVKPSVFFIRDHI
                     AAPQEHTYSFLLHVGPEGQIETADGVIQSSSPGASLEAHVFAPGGIRLAAGAYPGAEE
                     YGPYASATTGRGRQMTITSVLVPRRTPVTIVNGGFEQGFTGWQRRDMPGFTENHVIDH
                     EAPHAGSASGRIDNGGYYYTRHIPVSPGTQVTARWWARCTSAEGASSVLYYWRSGTAF
                     ASTPGPSAVVDEWAPFELTDTVPEGAEEVCLALQFFGQGQCWYDDVEMVTDAPVPVSG
                     PARVRPIDDGQKGAVAEVDGAIHVLVCGSTGHETRLEAAGHVLTTDAELAVVSFIGDG
                     ARAFVVRGTRVEADGRPVRLDPGEWRLQRGF"
     gene            2046..>2621
                     /locus_tag="PLQ54_10990"
     CDS             2046..>2621
                     /locus_tag="PLQ54_10990"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOS93830.1"
                     /translation="MSGTLYWVMLASLPKVGPGAFRRLVDYFGSAEEAALASAEDHAR
                     AGILKPGQIDRLAKQRAQLGHLEHYLDEISFDGIRILTWESPDYPTLLRGLRSAPPVL
                     YARGDPGAVEETTMAVIGTRSPTDEGRRMAYALAAKLAELGVTIVSGLADGIDTAGHR
                     GALDAGGLTAAILGSGLLRISPKESEELAGEV"
BASE COUNT          423 a          906 c          878 g          414 t
ORIGIN      
        1 atcgatgcga cgctcgacat ggccgagccg taccacaact tcaccgccgt catcgccacc
       61 gggttgtgcg tgggagccat cgcgctgcat ggtgaggacg cccgcgccga cgcatggatc
      121 agccacgcca tcgcccgggc gaagctcaac ttcgacgccc agggcaagga cggcggcgcc
      181 atggagggcc ccatgtacgg gacctacgcc gccagcatgt tcgccgacat gatctgggca
      241 ctgacgacgg ccggcgtcga gaacgacctg gcggcacacc catacatcga gtcgatgccg
      301 cgctactgca tcagcctgct cgatcccggg acacgcagac agccctgttt cggcgatggt
      361 ggtcccaccg ccgggtttgg cgagctcatg ctcatcctgg cgctgcgggg cagtgccgat
      421 gcggcctggt actgccagca gattggccag ctcgacgcca aggatgtgcg ccggttcctg
      481 gcactggatc ccgcccgaat caggcccgcc gaacccactt ggtcgccctc gggctgcttc
      541 gtcgatgtcg gatacgccat cttgcgcgac ggctactcgc gcgactcggc gttcatggcc
      601 ctcaagtgcg ggccgcccga ggccgacgtc ggccacaacc atttcgacca caacagcttc
      661 gtcatcagct tcgcgggcgc gtggcccgca tgggaccccg ggtaccgcag ctacttcgat
      721 ccccccaagc gccggtacac cacgagtacg ttgggccaca acaccatcgt cgtggatctc
      781 gacgatgcct acctggccag tcaggaagcc gcgcagcctg gccacgatca gctgcacgtc
      841 aaccgcgggc ggatcaccga gttctatgcc ggccccggct ttgactacgc cctcggcgac
      901 gcggcgaacg cctacaacac cgatgcggcc catctgctcg atgcgttcac gcgccagatc
      961 gtcttcgtga aacccagtgt gttcttcatc cgcgatcaca ttgcggcccc gcaggaacac
     1021 acctactcgt tcctgctcca tgtgggtccc gagggccaga tcgagacagc ggatggcgtg
     1081 atccagagca gcagccccgg ggcgtcactc gaagcgcacg tgttcgcgcc cggcggcatc
     1141 cgtctggccg cgggggccta cccgggtgcg gaggagtatg gcccctacgc atcggcaacg
     1201 accgggcgtg ggcgacagat gacgatcacg agcgttctgg tgccgcgccg gacaccggtg
     1261 accatagtca acggtgggtt tgagcagggc ttcaccggct ggcagcggcg ggacatgccc
     1321 ggtttcaccg agaaccatgt catcgaccac gaggctcccc acgcgggcag cgcgtcgggc
     1381 cgcatcgaca acggcggcta ctactacacc cgccacatcc ccgtgtcgcc cgggacccaa
     1441 gtcacggccc gatggtgggc ccggtgcacg agcgccgagg gtgcatcgag cgtgctctac
     1501 tactggcgga gcgggacagc cttcgcgagc acgccgggtc catcggccgt cgtcgatgag
     1561 tgggcaccat tcgagctgac cgacacggtc cccgaaggcg ccgaggaggt gtgcctggcc
     1621 ctccagttct tcggccaggg ccagtgctgg tacgacgacg tcgagatggt gaccgacgcg
     1681 ccggtgccgg tttccgggcc tgctcgcgtc cggcccatcg acgacggcca gaagggggcg
     1741 gtcgccgagg tcgatggcgc catccacgtg ctcgtctgcg ggtcgaccgg gcacgagact
     1801 cgcctggagg ctgcggggca cgtactcacc accgatgccg agctggccgt ggtaagcttc
     1861 ataggcgatg gcgctcgcgc gttcgtcgtc cgcggcaccc gggtggaagc cgatggccga
     1921 ccggttcggc tcgaccccgg cgagtggcgg ctgcaacgcg gattctaggg actggcgccc
     1981 acggtgttgc cgttagcctt gtcccgggcg tgctgcgccg ccacggtcac ggaggggacc
     2041 cactgatgtc cggtacgctc tactgggtca tgctggcatc gctgccgaag gtggggcccg
     2101 gcgcgttccg tcgcctcgtc gactacttcg gctccgccga ggaagccgcg ctcgcatcgg
     2161 ccgaggacca cgcccgcgcg ggaatcctca aacccgggca gatcgaccgg ctcgccaaac
     2221 agcgggccca gctcggccat ctcgagcatt acctcgacga gatcagcttc gatggcattc
     2281 gcatcctcac atgggagagc ccggactacc ccacactcct gcgcgggctg cggtctgctc
     2341 ctcccgtgct gtacgcacga ggagacccgg gggccgtgga ggagaccacg atggcggtca
     2401 ttggcacccg gtctcccacc gatgagggcc gccggatggc ttacgcgctt gcggcgaaac
     2461 tggcggagct tggtgtcacc atcgttagcg gcctggccga cgggatcgac acggccgggc
     2521 atcggggggc gctcgatgcg ggtggcctga cggcggccat tctcggttcg gggctgcttc
     2581 ggatcagccc gaaggagagc gaggagctgg caggcgaggt c
//
LOCUS       DAOSPL010000585         2618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39237_length_2618_cov_3.328911, whole genome shotgun sequence.
ACCESSION   DAOSPL010000585 DAOSPL010000000
VERSION     DAOSPL010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39237_length_2618_cov_3.328911"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            290..1120
                     /gene="modA"
                     /locus_tag="PLQ54_10995"
     CDS             290..1120
                     /gene="modA"
                     /locus_tag="PLQ54_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOS93831.1"
                     /translation="MIPVARGKAMWVLCCTAVVLVATLMGCRSSARTGADVVQVRAGP
                     VLGDAVCELAEAFEAHRQGIRVVTNATCPLCMMPLRHTEPAAFDVVVSIGSPDMDRMV
                     HAGLIDPDSVRDIGGTRLALVVRRGSRASVARVEDLAEPGIRSVLLGDPSNVSVGHYA
                     EQALRAAGVWDSVKEKITGCRTGCEVLKTLALGDADAGFVYEFCLTNKTETSAELACL
                     VPLESHDPIRVLMGITKGAAAPAAAAFRDFALGDEGSQVLMRHGLKPERPGGQSGARG
                     "
     gene            1143..1418
                     /locus_tag="PLQ54_11000"
     CDS             1143..1418
                     /locus_tag="PLQ54_11000"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOS93832.1"
                     /translation="MPVVIQIPTPLRRFTGGRRTVTVEAHTVGAALDELTGIFPDVRA
                     GLQRPDGSLRSDARLFVGAEDIRGLGGLDTPVSDGDVIAIIPPVAGA"
     gene            1443..2465
                     /locus_tag="PLQ54_11005"
     CDS             1443..2465
                     /locus_tag="PLQ54_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOS93833.1"
                     /translation="MEDLSRYARQSILAQVGTEGQARLGRSRVAVAGCGALGSASATL
                     LVRAGVGYVRIIDRDFVETSNLQRQTLYDEDDVASGMPKAAIAAQKLQRANTSVRVEP
                     RVADLTASSAEALLRDVDVIVDAIDNFEARFLVNDVAVKHSIPWVYGAVIATYGLTMT
                     IRPGETACLRCLLPEAPAPGSVDTCATAGILGPVVECIAALQVTEALKLLLGRTDELC
                     GGLAQFDVWDRELSVVDVPRQSDCPTCVQARYDYLRAESTAETVSLCGREAIQISVPR
                     TQPLVLTELAARIRGFAPVQESPFMVRFCVDGREINVFADGRAIIKGTTDEALARKLY
                     AEYVGT"
     gene            2472..>2618
                     /locus_tag="PLQ54_11010"
     CDS             2472..>2618
                     /locus_tag="PLQ54_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOS93834.1"
                     /translation="MIYLDNAATTFPKPEGVYSAMDRFLRTSCANPGRSGHRMAVESQ
                     RVVSQ"
BASE COUNT          401 a          770 c          978 g          469 t
ORIGIN      
        1 gccgggtggc cccgcggtcg gccttgtccc accacggttc gcacgcggca cagaaacacg
       61 tcatactccg tgaggccgac gccaatgtaa tgcggtgaat ggggggagca cagccggccg
      121 ggagggcccg atggatcgga cgtaccgtgg caaccttcgg gccgacggtt ggtttggtcg
      181 cctccgccgt gtggctggtg ttgtaccatg ggctcggggc ccgcgtgggt ttggcggcga
      241 cgtcgtggcg tgtcgccgtg ttcccgttgc cgcgagggcc tggaggcggg tgatacctgt
      301 ggcacgcggc aaggcgatgt gggtgctgtg ctgcacggcg gtggtgcttg tcgccacgct
      361 gatgggttgc cggtcgtcgg cgcgaaccgg agctgatgtg gttcaggttc gagcgggacc
      421 ggtgctgggc gatgcggtct gcgagctggc cgaagcgttc gaggcgcacc ggcaggggat
      481 tcgagtggtc accaacgcga cgtgcccgtt gtgcatgatg ccgctgcgcc acaccgagcc
      541 tgccgccttc gatgtggtgg tttcgatcgg gtcgcctgac atggaccgga tggtccacgc
      601 cggcctgatc gatccggata gcgtgaggga catcggtggc acgcgattgg cgctggtcgt
      661 gcggcgaggc tccagggcga gcgtggcccg ggtcgaggat ctggccgagc cggggatccg
      721 gagtgtcctg ctgggtgacc cgtccaacgt gtctgtaggt cactacgcgg agcaggccct
      781 gcgcgccgcg ggtgtatggg attcggtgaa agagaagata actgggtgtc gcaccggatg
      841 tgaggtgctc aagacacttg ctctcggaga cgctgacgcg gggttcgtat atgagttctg
      901 tctgacgaac aagacagaga ccagcgccga gctggcctgc ctggttccgc tcgagagtca
      961 cgacccgatt cgcgtcctga tgggcatcac caagggagcc gcagcgccgg ctgcggctgc
     1021 tttccgagac ttcgcgctcg gcgacgaggg aagccaggtc ctcatgcgcc atgggctgaa
     1081 gccggagcga cccggcggcc agtctggggc gaggggctag cggcccgata ggaggtagag
     1141 cagtgccggt tgtaatccag attccgacgc cactacgtcg attcacgggt ggccggcgca
     1201 ccgtgacggt tgaggcccac acggtggggg cagctctgga cgaactcacg gggatattcc
     1261 ccgacgtgcg ggccggcctg cagaggcccg atggttctct gcggtctgac gcccggttgt
     1321 tcgtcggtgc cgaggacatc cgagggctcg gcgggttgga cactcccgtg agcgacggcg
     1381 acgtcattgc catcattccg cctgtggcgg gcgcgtgagg cgccggaagg gtcggggccg
     1441 tcctggagga cctatcgcgg tacgcgagac agtcaatcct ggcgcaagtg gggacagagg
     1501 gtcaggctcg gttgggccgg agtcgcgtcg cggtggccgg gtgcggtgct cttggctccg
     1561 ccagcgcgac ccttctagtg cgagctggcg tgggctacgt ccgcatcatc gaccgcgact
     1621 ttgttgaaac ctcgaaccta cagcgtcaga cactctacga cgaggacgac gtggcatcgg
     1681 gcatgcccaa ggcggccatt gctgcgcaga agctgcagcg ggccaacact tcggttcgtg
     1741 tcgagccgcg ggtggcggat ctcacggctt cgtctgccga ggcgctgctg cgggatgtcg
     1801 acgtgatcgt cgatgccatc gacaacttcg aggcgcgctt cctggtcaat gacgtcgctg
     1861 tgaagcactc gataccatgg gtctacgggg ccgtcatagc gacctacgga ctcaccatga
     1921 ccatccggcc cggcgagact gcctgtctga ggtgcctgtt gcccgaagcg ccggcaccgg
     1981 ggtcagtgga cacctgcgcc acagcgggca ttttggggcc ggtggtcgag tgtatcgcgg
     2041 ccctacaggt gaccgaggcg ctgaagctgc tgctcggccg gacggacgag ctgtgtggcg
     2101 ggctggctca gttcgacgtg tgggatcgtg agctctcggt cgtcgacgtg ccgcgccaga
     2161 gcgactgccc gacgtgcgtt caggcccgct acgattacct gcgggccgag tcgacggcag
     2221 agacggtgtc gttgtgcggg cgtgaggcga tccagatcag cgtgccccgg acgcagccgt
     2281 tggtgctgac ggagctggcg gcccggatcc gcgggttcgc gccggtgcag gagagtccat
     2341 tcatggtccg gttctgtgtg gacggccgcg agatcaacgt cttcgccgac ggacgcgcca
     2401 ttatcaaggg gaccaccgat gaggcgctgg cgcggaagct gtatgccgaa tacgtgggga
     2461 cgtagtccaa gatgatctac ctcgacaacg cggcgacgac cttcccgaag cccgagggtg
     2521 tctactcggc catggaccgg ttcctgcgca catcatgcgc gaacccgggc cggagtgggc
     2581 accggatggc tgtggagtcc cagcgggtgg tctcgcag
//
LOCUS       DAOSPL010000586         2618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39246_length_2618_cov_2.215373, whole genome shotgun sequence.
ACCESSION   DAOSPL010000586 DAOSPL010000000
VERSION     DAOSPL010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39246_length_2618_cov_2.215373"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLQ54_11015"
     CDS             <1..487
                     /locus_tag="PLQ54_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93835.1"
                     /translation="AALSVAPGDGSIESAVEAGVRAREVARASLAAQGDSSRQAEIAL
                     DATFLTAMALAGSDAARPAGFEPSGAADQDASRWFAAHYLSRLFGHLVARDTADVVGT
                     PGLPDVQARCRLVEAAERVCAEAVRDCPWAGAPQGDPAPLLRGLITGALERLRAAGGP
                     G"
     gene            484..1851
                     /locus_tag="PLQ54_11020"
     CDS             484..1851
                     /locus_tag="PLQ54_11020"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HOS93836.1"
                     /translation="MNARVVVRADGDETVLLPANGPPVVVLDWVSRRRNVELDLHRLS
                     TRLPQPLSDSAQDLLEVTAALVMGDHLVPRGANEQWVRSLTFAIPVRLPGTWEAAAPA
                     LTSLFHLMTGDNIRLEFRPRACARVTRTSQAGLRSAADCVSLLSGGIDSFAGAVASLE
                     EGRRPLFVMHRSSNATTLEAQRRVVGSLRSHYGAAFEVIGVSLGVSRVREPEFVLPSR
                     SDREPSRRARSLWYLALAAGVAWSAGIEEIVVPENGIMALHVPMGPSRVGGLSTRTTH
                     PEVLACFASVASEILDHRLGIGNPLLGHTKAEVVRDILRWRVSEAEIRRTESCWQAGR
                     HPRPCGGCVPCLQRQLALATAGVGPEAQQMELLGNPESHRGTDGFRNLADLLGMVAEF
                     SRLPDDRLLTRHPALGQAGLPPAAAIGLYRRFAAEVVELGHTRYPALGRMLGTGQMAE
                     GRGGR"
     gene            1927..>2618
                     /locus_tag="PLQ54_11025"
     CDS             1927..>2618
                     /locus_tag="PLQ54_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93837.1"
                     /translation="MQPRLGWGLVRVPGHHSAAVSWAVALTLLMPAFGAGQTVLGVEG
                     TRFTINGEPTFLLGISYYGGAAAPAETVRRDLDDLAERGINWIRVWATWVSGDENTSA
                     VTGSGEPRPPYFDQLVELVRLADERGIVVDVTLTRGPLGPRPTEEALTKVEDHCRAAS
                     VLAAALRGFRNVYIDMANERDVRDARFVPYEELARIRAAIRDVDPERLVTASGVDDGG
                     AKHLKGHLEVAG"
BASE COUNT          342 a          857 c         1014 g          405 t
ORIGIN      
        1 ggccgcgctg tcggtggccc caggcgatgg ctcgatcgag tcggccgtcg aggccggcgt
       61 ccgagcccgc gaggtcgccc gggccagtct ggccgcccaa ggcgactcga gccgtcaggc
      121 cgagatcgcc ctcgatgcca ccttcctgac ggccatggcg ctggccggga gcgacgccgc
      181 gcggccggcg ggcttcgagc cttcgggcgc tgccgaccag gatgccagcc ggtggttcgc
      241 ggcccactac ctgtcgcgac tgttcgggca tctggtggcg cgcgacacgg ccgatgtggt
      301 gggcaccccg gggctgccgg acgttcaggc ccgatgccgg ctggtcgagg cggcggaacg
      361 ggtctgcgcg gaggccgtcc gcgactgccc gtgggccggc gcgccccagg gcgacccagc
      421 accgctgctc cgggggctca tcaccggcgc gctggaacgg ctgcgagcgg ccgggggccc
      481 cggttgaacg cacgagtagt cgtgcgggcc gacggggatg agaccgtgct gctgccggcc
      541 aacggccctc ccgtggtggt gctcgactgg gtgtcgcggc ggcgcaacgt tgagctggac
      601 ctccatcggc tctcgacccg gctccctcaa ccgctcagcg actcggcgca ggacctgctc
      661 gaggtcaccg cggcgctggt gatgggcgat cacctggtgc cgcggggggc gaacgagcag
      721 tgggtccgct cgctcacctt cgccatccct gttcggctgc ccggcacctg ggaggccgcc
      781 gcgccggcgc tcacctcgct gttccacctg atgacggggg acaacatccg tctcgagttc
      841 cggccaaggg cctgcgcccg tgtaacccgc acgagccagg cggggctgcg gagcgcggcg
      901 gactgcgtga gtctgctttc gggcgggatc gactcgttcg ccggggcggt ggcgtcgttg
      961 gaggaagggc ggcggccgct gttcgtgatg catcgctcgt cgaacgccac gaccctggag
     1021 gcccagcggc gtgttgtcgg gtcgctgcgc agccactacg gtgccgcctt cgaggtcatt
     1081 ggggtgtcac tgggcgtctc gcgggtccgg gagcccgagt tcgtgctccc gtcgcgaagc
     1141 gaccgggagc cgtcgcgccg ggcccgatcg ctctggtacc tggcgctggc cgctggggtg
     1201 gcgtggtcgg cggggatcga agagatcgtg gtgcccgaga atggcatcat ggcgctgcac
     1261 gtgcccatgg ggccatcgcg cgtgggcggg ctgtcgaccc gcaccacgca tcccgaggtc
     1321 ctggcttgct tcgcgtcggt ggcgtcggag atcctcgatc accgcctcgg gatcggcaac
     1381 ccgctcctcg gccatacgaa ggccgaggtc gtgcgggaca tactgcgatg gcgggtgagc
     1441 gaggcggaga ttcgccgaac cgagtcgtgc tggcaagccg ggcggcaccc gaggccatgt
     1501 ggcgggtgcg tgccgtgcct ccagaggcag ttggccctcg ccacggctgg tgtggggcct
     1561 gaggcgcagc agatggagct gcttggcaac ccggagagcc acaggggcac cgacgggttc
     1621 cgcaacctcg cggacctgct gggcatggtg gcggagttct cccggctgcc cgacgatcgg
     1681 ttgctgacga ggcatccggc gctgggccag gccggactgc cccccgccgc ggccattggg
     1741 ctgtatcgac ggttcgcggc cgaggtcgtg gaactcggac acacacggta ccccgccctg
     1801 ggccggatgc taggcacagg gcagatggca gagggcagag gaggacgttg agaggcgggt
     1861 cggtaccgcg gcccagggca tgcgcggcgc aggtgggtcg tcgacgccga gcgaagccgc
     1921 tgccggatgc aaccgaggct gggctggggg ctggtgcggg tgccaggaca ccactccgcc
     1981 gcggtctcgt gggccgtggc actcacgcta ctgatgcctg ccttcggggc aggccagaca
     2041 gtcctgggcg tcgaggggac ccgatttacg atcaacggcg agcccacttt cctgttgggc
     2101 atcagctact acggcggcgc cgcggcgccg gctgagaccg tccggcgcga tttggacgac
     2161 ctcgcggagc gcggcatcaa ctggatccgc gtgtgggcca cgtgggtgtc tggcgatgag
     2221 aacacgtcgg cggtcaccgg ttcgggggag ccgcgtccgc cgtacttcga ccagcttgtc
     2281 gagctggtcc ggctggccga tgagcgtggc atcgtggttg acgtcactct gacgcggggc
     2341 ccgctggggc cgcgtcccac cgaggaggcg ttgaccaagg tggaggatca ctgtcgcgcg
     2401 gcgagcgtgc tggccgcggc acttcggggc ttccggaatg tctacatcga catggccaac
     2461 gagcgcgatg tccgcgatgc gcgcttcgtg ccctatgagg agctggctcg gatccgggcc
     2521 gccattcggg atgtggatcc cgagcgcctg gtcacggcat cgggagtcga tgatgggggc
     2581 gccaagcacc tgaagggtca cctggaggtc gcgggtga
//
LOCUS       DAOSPL010000587         2617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39263_length_2617_cov_2.878220, whole genome shotgun sequence.
ACCESSION   DAOSPL010000587 DAOSPL010000000
VERSION     DAOSPL010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39263_length_2617_cov_2.878220"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="PLQ54_11030"
     CDS             <1..944
                     /locus_tag="PLQ54_11030"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOS93838.1"
                     /translation="GTAVIYISHRLEEVVEIGDRITVLRDGANVATLSRAEADIPRLI
                     RLMVGREVSDAPLREPMAPGAELLRVDGVTRRGVLAPCSFSVRAGEVVGVAGLVGSGR
                     TELVRAVFGADSPDAGTVSVGGQVVPPGSPAASVAAGLALLPEDRKADGLALNLSVRA
                     NVSLACLAQLIRLGLIDRRREASLAGEQITSLNIRLRDAAQPAGSLSGGNQQKVLLAR
                     WLASRARVFLFDEPTRGIDVGAKFEIYALINRLTREGAAIVMVSSYLPELLGMSDRIL
                     VMRRGAIVAEMPRCEATQERIMHFAALQNIGAEGASA"
     gene            941..1975
                     /locus_tag="PLQ54_11035"
     CDS             941..1975
                     /locus_tag="PLQ54_11035"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS93839.1"
                     /translation="MSETIQEAGGLWRTHRRTILNVADRLGPLLSLGVVVAFFAWRGS
                     NFLTWGNGLNIGVQTAVFGILALGETFVIIAGGIDLSVGAVTALAGVVAAVTMKSGHS
                     PLEGILAGLAVGGGVGLTNGLISAYGKLPSFVVTLGTMLIARGLALSVAGGVNISGLP
                     DAFTNCMGGTFYIGGKTAEAVGTWREVREPVLILLVAALGAHLLLSRTRMGRYTYAMG
                     SNAEATRLSGVNIRAYQTAIFTLMGLLAGLAGLVQATRISAGSPTTAEWYELSAIAAT
                     VIGGTSLMGGEGGIAGTLIGALMLGVVANGLDLCNADPYWQKIATGAIIVFAVFLDRL
                     RARARKSLQE"
     gene            2118..>2617
                     /locus_tag="PLQ54_11040"
     CDS             2118..>2617
                     /locus_tag="PLQ54_11040"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOS93840.1"
                     /translation="MRTHPFASAAVVLVASFALGIGLSACKSEQQPEGPPPLPGGAQV
                     KGDVEKTKAAGAGKRIAVVPKGTAHSFWKAVEAGAKAAGNEFGCEILWKGPETETQVD
                     RQIEIIRNFVTQKVDAIVMAACDASALVPELKKAKEAGITIVTIDSGIEDATIPVSFV
                     ATDNVAA"
BASE COUNT          382 a          856 c          922 g          457 t
ORIGIN      
        1 cagggaccgc ggtgatctac atctcccatc ggctggagga ggtcgttgag atcggcgacc
       61 gcatcacggt actgcgcgat ggcgcgaacg tggccacgct gtcgcgcgcc gaggccgaca
      121 ttcccagact gatccggctg atggtgggtc gcgaggtctc ggacgcaccg ttgcgcgagc
      181 ccatggcgcc gggtgctgag ctcttgcggg tcgatggtgt gacccgccgc ggcgtcctgg
      241 cgccgtgctc gttctcggtt cgagcgggcg aggtggttgg cgttgcgggc ctggtcggat
      301 cgggccggac cgagctcgtc cgcgccgtgt ttggcgccga ttcgccggac gcgggcaccg
      361 tgtcggtcgg cggtcaggtc gtgccgcccg ggtcacctgc cgcttcggtg gccgcggggc
      421 tggcactgct acccgaggac cgcaaggccg acggtctggc gttgaacctg tccgtgcgag
      481 ccaacgtgtc tctggcctgc ctggctcagc tcatccgcct tggtctgatc gatcgccgcc
      541 gggaggcctc actggcgggc gagcagatca cctcgctcaa tatccggctg cgcgacgcgg
      601 cccagccggc gggaagtctc agcggcggca accagcagaa agtgctgctc gcgcgatggc
      661 tggcgagccg cgcccgcgtg ttcctgttcg atgagccgac ccgcggtatc gatgtgggtg
      721 ccaagtttga gatctatgcg ctgatcaacc ggctcacccg tgagggcgcc gccatcgtca
      781 tggtgtcgtc atacctgccc gagctgctgg ggatgagcga ccggatcctg gtgatgcggc
      841 ggggcgccat tgtggcggag atgcctcggt gcgaggccac gcaggagcgc atcatgcact
      901 tcgcggctct ccaaaacatc ggagcggagg gtgcatcagc gtgagcgaga ccatccagga
      961 ggccggcggg ctgtggcgga cccaccggag aacgatcctg aacgtcgcgg accggctggg
     1021 acccctgctc agcctgggcg tcgtggtcgc cttcttcgcg tggcgtggca gcaacttcct
     1081 tacatggggg aacgggttga acatcggcgt gcaaacggcg gtgttcggca tactggccct
     1141 gggggagacc ttcgtcatca ttgcgggggg catcgacctc tcggtcggcg ccgtgacggc
     1201 tctggccggc gtggtcgcgg ccgtcaccat gaagtcgggt cacagcccgc tggagggcat
     1261 tctcgccgga ctcgccgtgg gcggcggcgt cgggctaacc aatggtctga tctcggccta
     1321 cggcaagctg ccgtccttcg tcgtcacgct cggtaccatg ctcatcgccc gggggctcgc
     1381 cctcagtgtt gcggggggag tcaacatctc agggttgccg gatgccttca ccaactgcat
     1441 ggggggcacg ttctacatcg gcgggaagac cgccgaggcc gtcggcacct ggcgcgaggt
     1501 ccgtgagccc gtgctcatcc tgctcgtggc cgcattgggc gctcacctgc tgctctcccg
     1561 cacgcggatg ggccggtata cgtacgccat gggcagcaac gccgaggcga cgcgtctgtc
     1621 gggcgtcaac atccgggcct accagacggc catcttcacc ctgatgggcc tgctggccgg
     1681 gctggccggg ctggtccagg ccacccgcat ctccgccggc agccccacca cggctgagtg
     1741 gtatgagctc tcggccatcg cggccaccgt catcggcggg acgtcgctca tgggcggtga
     1801 gggcgggatc gcaggcacat tgatcggcgc cctgatgctc ggcgtggtgg ccaatgggct
     1861 cgatctgtgt aacgcggacc cgtattggca gaagatcgcc acgggggcca tcatcgtctt
     1921 cgccgtgttc ctcgatcgcc tccgcgcgcg ggcgcgcaag tcgcttcagg agtaggtgca
     1981 cagggccggg ctacgcccga acaccgtccc ctccttggcg gccgatgcgc cccctgagta
     2041 tcatgaggcg gccgcgcccg tttgccattc cacgcgagct tcccgtgggc ccaacggtgc
     2101 ggtgaggagg atgctctgtg agaacccatc cgtttgcctc ggccgccgtg gtgctcgtgg
     2161 cctctttcgc cctgggcatc ggcctgtcgg cctgcaagtc cgagcaacag cctgaggggc
     2221 caccgcctct tcccggtgga gcccaggtca agggagatgt ggagaagacc aaggcggccg
     2281 gtgccggcaa gaggatcgcg gtggtcccca agggcaccgc gcacagcttc tggaaggccg
     2341 tagaggccgg ggccaaggcc gccggcaatg agttcggctg tgagatcctt tggaagggcc
     2401 ccgagaccga gacccaggtc gatcgccaga tcgagatcat ccgcaacttc gtgacccaga
     2461 aggtcgacgc gatcgtcatg gcggcgtgcg atgccagcgc cctcgttcct gagctgaaga
     2521 aggccaagga ggccggcatc accatcgtca ccatcgactc tggcatcgag gatgccacga
     2581 tccccgtttc cttcgtcgcc accgacaacg tggccgc
//
LOCUS       DAOSPL010000588         2613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39334_length_2613_cov_3.206411, whole genome shotgun sequence.
ACCESSION   DAOSPL010000588 DAOSPL010000000
VERSION     DAOSPL010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39334_length_2613_cov_3.206411"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="PLQ54_11045"
     CDS             <1..1003
                     /locus_tag="PLQ54_11045"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HOS93841.1"
                     /translation="DVPPMVEPMEAGLILARHTRRPGPPFAWHVDQVPYLIEMGEILG
                     LPGWYTWGAICFAHPLRFDRDVVARFVARAKLGDPVGLTAMPVAGVTTPITVAGFVTM
                     CAAELVATWIAARAINPHAPFTGSIWGGALDMRTGDVSYSSFDAMFYALAASEFLRRW
                     TGHLIPVGGGEYSSAREPGYYAAMEKAHKAMTIAAFTGQPPSIGSGMLESGRTLCLVQ
                     LLLERELTEGVRIYSRDIEVSTATLCLDDILAVEHGLERSFLSTDSVLRDYRQYSWCP
                     PTLERDGWRGAQTDERVLQRLQARVDDLIASHVDPPVDEDRLAAAARVARRARTELL"
     gene            complement(998..1822)
                     /locus_tag="PLQ54_11050"
     CDS             complement(998..1822)
                     /locus_tag="PLQ54_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS93842.1"
                     /translation="MLLSVTTDYATDSGCPEPALRRIAEAGFTHIHWCHHWSSDFVYS
                     HAEVDQILRWMKGFGLKLTDLHGSIGPEKNWGSPREYERLAGVELVLNRIEMTAWLGG
                     DVVIMHLPAGCPEGDEEAWTRFRRSMDALEPYASIHAVRIAIENGGSDASFDAIERVF
                     GMYGPEFVGLCYDSGHGNLFPGGLDRLAKLADRLISVHLHDNQGTGDDHNLPFSGTVD
                     WERLTGILARSGYTKWISEEVSIRNSGLAEEPAFLTKAFETAQRLQEMVDATRGTL"
     gene            2034..2393
                     /locus_tag="PLQ54_11055"
     CDS             2034..2393
                     /locus_tag="PLQ54_11055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOS93843.1"
                     /translation="MSHRGDSAMGVSRRGFLKSAAVGAAALGLGTAVTPQANARPLGA
                     NERINIGMIGVGGRGSWLAGDILAKAKDGQNIAITAVCDVWDVRAQDVVNRTKNETGV
                     DAKRFRDYRELIEVPDL"
BASE COUNT          437 a          934 c          816 g          426 t
ORIGIN      
        1 cgacgtcccc cccatggtcg agccgatgga ggccggtctc atcctcgccc ggcacacgcg
       61 acggccggga ccgcccttcg cctggcacgt ggaccaggtg ccctacctca tcgagatggg
      121 cgagatcctc ggcttgccgg gctggtacac ctggggcgcc atctgcttcg cccaccccct
      181 ccgcttcgac cgtgacgtcg tcgccagatt cgtcgcgcgg gccaagctcg gcgaccccgt
      241 cgggcttacc gctatgcccg tggcgggcgt gacgaccccc atcaccgttg cgggcttcgt
      301 gacgatgtgc gccgcggaac tcgtggccac ctggatcgcc gctcgggcca tcaacccgca
      361 cgcacccttc accggctcga tctggggcgg cgcactcgac atgcgcaccg gcgatgtgag
      421 ctactcatcg ttcgatgcca tgttctacgc cctggccgcg tccgagttcc tgcgacgatg
      481 gaccggccac ctgatcccgg tgggcggcgg ggagtactcc agcgctcggg agccgggcta
      541 ctacgccgcc atggagaaag cccacaaggc catgaccatc gcggcattta ccgggcagcc
      601 gccctccatc ggatcgggca tgctcgaatc gggcaggacc ctctgcctcg tgcagttgct
      661 gctggaacgc gagctcaccg agggcgtccg catctactcg agagacatcg aggtctccac
      721 tgcaactctg tgcctcgacg acatcctcgc cgtcgagcac ggcctcgaac gcagcttcct
      781 gagcaccgac tccgtcctcc gtgactaccg ccagtactcc tggtgccccc caacgctcga
      841 gcgcgacggc tggcgcggag cccagaccga tgagcgcgtc ctccagaggc ttcaagcccg
      901 ggtcgatgac ctcatcgcca gccacgtcga tccgcccgtc gatgaggacc gcctcgccgc
      961 cgccgcccgg gtggcccggc gcgcccggac cgagctccta tagcgtccca cgggtcgcgt
     1021 ccaccatctc ctggagcctc tgcgccgtct cgaaggcctt ggtcagaaac gccggctcct
     1081 cagcgagccc cgaattgcga atcgagacct cctcgctgat ccacttcgtg taacccgatc
     1141 gtgcgaggat tccagtcagc cgttcccagt ccactgtccc cgagaaagga aggttgtggt
     1201 cgtcgccggt gccctggttg tcgtggaggt gcaccgagat gagtcgatcg gccagcttgg
     1261 ccaggcggtc cagccccccc gggaacaggt ttccgtgccc ggagtcgtag cacagcccca
     1321 cgaactccgg cccgtacatc ccgaagaccc gctcgatggc gtcgaaactg gcgtccgagc
     1381 ccccattctc gatcgcgatc cgcaccgcgt ggatcgacgc atacggctcg agcgcatcca
     1441 tggaacgccg gaagcgcgtc caggcctcct catcaccctc cgggcaccct gccggaaggt
     1501 gcataatcac cacgtcgccc cccagccagg ccgtcatctc aatccgattg agaaccaact
     1561 cgacgcccgc cagacgctca tactcacgcg gggagcccca gttcttctcc ggaccgatgg
     1621 atccgtgcag atcggtcagc ttcagcccga atcccttcat ccagcgaagg atctggtcca
     1681 cctctgcgtg ggagtagacg aagtccgaag accagtggtg gcaccagtga atgtgtgtga
     1741 agcccgcttc ggcgatgcgc cgaagcgcgg gctcgggaca gcccgaatcg gtggcgtaat
     1801 cggtggtcac ggaaagaagc atggtccacg aacctccagg tctatcgccg ccacggcggc
     1861 gcgtatgcgt ccttcgcccg ggcgctgtgc gcgcccttcc tcgccggagc ttcgcgccgt
     1921 cgcaggtcgc gtctcgccac cgcgcgaatg gtttggtggc ggcgctttcc cgccgcatca
     1981 atctcgcccg ctgggtgcgg acgggatcgg tctccactcg ggaggttcgg cacatgagcc
     2041 atcgtggcga cagcgcgatg ggggtcagcc gtcgcgggtt cctcaagtcg gccgccgtgg
     2101 gcgccgcggc actcggactg gggaccgcgg tcaccccaca ggccaacgcg cgccccctgg
     2161 gcgccaacga gcggatcaac atcggaatga tcggagtcgg cggccgcgga agctggctgg
     2221 ccggtgacat tctggccaag gccaaagacg gccagaacat cgcgatcacc gctgtctgcg
     2281 acgtgtggga tgtccgcgca caagacgtgg tgaaccgcac caagaacgaa acgggcgtcg
     2341 acgccaagcg attccgtgac taccgcgagc tgatcgaggt gcccgacctc taggcggtta
     2401 tcatcgccac gccggaccac tggcatgcca agcagtccat cgacgccatg cgcaagggac
     2461 tcgatgtcta ctgcgagaag cccatgaccc tctattggca cgaggccaaa gaggtctctc
     2521 gagtcgccga ggagaccggg gccgtgttcc agtgtggtgc gggctcggcc tcggacggca
     2581 actggtggac cgtcggtgac atcgtccgcg agg
//
LOCUS       DAOSPL010000589         2608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39422_length_2608_cov_2.993341, whole genome shotgun sequence.
ACCESSION   DAOSPL010000589 DAOSPL010000000
VERSION     DAOSPL010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39422_length_2608_cov_2.993341"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="PLQ54_11060"
     CDS             <1..951
                     /locus_tag="PLQ54_11060"
                     /inference="COORDINATES: protein motif:HMM:NF020772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOS93844.1"
                     /translation="QYESQCEPTAERHTDPNQAYLTSSAPLGMNPVAFFVRDEDTALQ
                     VWSGELGYPGDGWYLDFHKKYFPGGFRFWRVTRAKSDLAEKDLYDWRQAQQRIPSHAD
                     HFVSLVASGAAWDGTGAPPIRCCPFDAELFGHWWHEGPEWLEAVLERAHDHANVHAVS
                     LGDYLERHEPEKGVGLPEGSWGEGGYHFIWLNQMTSWTWTRIYEAEDRMAGLLARAPA
                     DSAGLLDRVLKQAARELLLLQSSDWQFLISTWNARDYAELRFEGHRESFARLALIAET
                     MLGGAAPTEADVRFLEETEARDRLFADINLKWFRDLETPV"
     gene            985..1731
                     /locus_tag="PLQ54_11065"
     CDS             985..1731
                     /locus_tag="PLQ54_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93845.1"
                     /translation="MQDLIDLTLLRWAPAIPQGARAPRGRVRRAMQFLLAHARGRAHA
                     QTVVDYVRTMEPAIVLRLRDELLALPDEERNAYLADQTARLKAGLRRAMDDCRRRWGL
                     PVVALPGDAYYVDLPDGLTALDDPEEARARLPRDQWPAFDHMVRVLSEIDRALDEVPD
                     GGRGALVPLSDRLAARVKRRWGVRPARYDLSAEVAALLARREELGEALHTALHASEVP
                     SPRTYTGAGPGPDGATGSALVRGVAAATAE"
     gene            1771..>2608
                     /locus_tag="PLQ54_11070"
     CDS             1771..>2608
                     /locus_tag="PLQ54_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS93846.1"
                     /translation="MAPEPAVRAGVAKLDSSRGGLMPVRMGIIGTGGIANHHLGILAA
                     MPDRVALVAYCDPVRERAQNAADQFGGKAYSDAAAMYDAEELDAVGVFVPPIAHGAIE
                     TMACERGIHMLIEKPCALDMTTGRTIEQAVRESGVITAIAYKYRWDRHVRQAREMLRS
                     RRIGLVHGWFWCGMPGAPWWRKDAESGGQIVEQTTHIVDMARYLVGEVAEVQAWFRRI
                     GEGYEGPPSDVDDVGTVNLIFENGALGNISNTCMLTHWGSSGLRVMAQGFTLEVNGNR
                     LSW"
BASE COUNT          414 a          872 c          923 g          399 t
ORIGIN      
        1 cagtacgaga gccagtgcga gcccacggcc gagcgccaca cggacccgaa ccaagcatat
       61 ctgaccagct ccgcgcccct gggcatgaac cccgtggcct ttttcgtccg cgacgaggac
      121 acggccctgc aggtctggag tggcgagctg gggtacccgg gtgatggctg gtacctggac
      181 ttccacaaga agtacttccc cggagggttt cgcttctggc gggtcacgcg cgccaagtcc
      241 gacctggcgg agaaggatct ctacgattgg cgccaggccc aacagcgcat cccgtcgcac
      301 gcggaccact tcgtgagcct ggtcgcttcc ggcgccgcgt gggatgggac gggcgcgccg
      361 ccgatccgct gctgcccctt cgacgccgag ctgttcggtc actggtggca cgagggcccc
      421 gagtggctgg aggccgtgct cgagcgggcc catgaccatg ccaacgtcca cgccgtcagc
      481 ctgggcgatt acctcgagcg ccatgaaccg gagaagggcg tggggctccc ggaaggctcc
      541 tggggcgagg gcggctacca cttcatctgg ctcaaccaga tgacgagctg gacatggaca
      601 cgtatctacg aggcagagga ccgcatggcg ggcctgctgg cgcgagctcc ggcagactcc
      661 gctggcctgc tcgaccgagt gctgaaacag gcggctcggg agctgctgct tctccagagc
      721 tcggactggc agttcctgat cagcacctgg aacgcgcgcg actacgctga gctgcggttc
      781 gagggccacc gggagtcctt tgcgcggctg gcgctcatcg ccgagacgat gctcggcggc
      841 gcagcgccga ccgaggccga tgtgcggttc ctcgaggaga ccgaagcgcg cgaccggctg
      901 ttcgcggaca tcaacctcaa gtggttccgg gacctcgaga cacccgtcta gccaccggcc
      961 cactcgcgac ggtatggagg tacgatgcag gatctgatcg atctcactct cctgcgctgg
     1021 gcgccggcaa tcccccaggg agcacgggcg ccgcgcgggc gggtccgccg ggccatgcag
     1081 tttctgctgg cccatgcccg aggccgcgcc catgcacaga ccgtggtcga ttacgtccgc
     1141 accatggagc ccgcgattgt gttgcggctg cgcgacgagc tcctcgctct gccggacgaa
     1201 gagcggaacg cctatctggc cgaccagacc gctcggctga aggcgggtct gcggcgggcg
     1261 atggacgact gccgtcgccg atggggactc ccggtcgtcg ccctaccagg cgatgcctat
     1321 tatgtagacc tgcccgacgg cctcacggcg ctggacgatc ccgaggaggc gcgcgcgcgc
     1381 ctgccgcgtg accaatggcc cgccttcgac cacatggtgc gagtgctctc ggagatcgac
     1441 cgtgcgctgg acgaggtgcc ggatgggggc cggggcgcct tggttccgct atcggaccgc
     1501 ctggcggccc gagttaagcg gcggtggggc gttcgccccg ctcgctatga cctctcggcc
     1561 gaggtggccg ccctgctggc tcgccgcgag gagctgggcg aggcactgca cacggccctg
     1621 cacgcctcgg aagtccccag cccgcggacg tacaccggcg cgggccccgg gcccgacggg
     1681 gccaccggca gtgcgctcgt ccgaggagtg gctgcggcga ccgccgaata gcgcatgccg
     1741 gccccaacgg gccagcagac cagggagccc gtggcgcccg agcccgcggt gcgggcaggt
     1801 gtcgccaagc tggactcgag taggggtggg ctcatgcccg ttcggatggg gatcatcggt
     1861 actggcggca tcgccaacca ccatctggga atcctggcgg ccatgcccga ccgggtcgcc
     1921 ctagtggcct actgcgaccc ggtccgggaa cgcgcccaga atgcggccga tcagttcgga
     1981 ggcaaggcct actccgatgc cgcagccatg tacgacgccg aggaactcga tgcggtcggc
     2041 gtgttcgttc cgccgatcgc ccacggcgcc atcgagacca tggcgtgcga gcgcggcatt
     2101 cacatgctca tcgagaagcc gtgcgcgctc gatatgacaa ccgggcggac catcgagcag
     2161 gcggtgcgcg agtccggcgt gatcacggcc atcgcctaca agtaccgttg ggacaggcat
     2221 gtgcgccagg cgcgcgagat gctccggtcg cgacgcatcg gcctggtgca cggttggttc
     2281 tggtgcggca tgccaggcgc cccctggtgg cggaaggacg ccgaatcggg cggccagatt
     2341 gtcgagcaga ccacacacat cgtcgatatg gcacggtacc tggtgggcga ggtcgccgag
     2401 gtgcaggcct ggttccgtcg cattggcgag ggctacgagg gtcccccgtc cgatgtcgac
     2461 gacgtgggca cggtgaacct gatcttcgag aacggtgcgc tgggcaacat ctcgaacacg
     2521 tgcatgctca cccactgggg gagcagtggc ttgcgggtca tggcccaggg gttcacgctc
     2581 gaggtcaacg gcaaccggct atcctggg
//
LOCUS       DAOSPL010000590         2607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39445_length_2607_cov_3.346395, whole genome shotgun sequence.
ACCESSION   DAOSPL010000590 DAOSPL010000000
VERSION     DAOSPL010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39445_length_2607_cov_3.346395"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PLQ54_11075"
     CDS             <1..252
                     /locus_tag="PLQ54_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012938780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOS93847.1"
                     /translation="APNAPDGSSTGRTSLGLPAGRGVVAVDPSVVPYGTRMWIEGYGW
                     AVAGDTGGAIKGRKIDLCFDDYGYAARFGTRTVTVRFFE"
     gene            265..1143
                     /gene="rsmA"
                     /locus_tag="PLQ54_11080"
     CDS             265..1143
                     /gene="rsmA"
                     /locus_tag="PLQ54_11080"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOS93848.1"
                     /translation="MTRPPEPRHDAIQLRTAAPAPPKRSLSQNFLVDRNTATAIVTAA
                     AIQPSESVLEIGPGRGALTFGLAEAAARLVAVERDDALAPWLAEQLASHGDRVRIIHA
                     DVLEADLAALLAPPGPWVVVGNLPYAITTPILLKLLQHARRFDRAIVMVQREVAQRWC
                     AGPGSRTYGAITVWCQYHCSCRVLRKVRPSCFRPSPSVESALVELRPHSDALPRARDE
                     ALLQQVTRAAFGQRRKRAATSLAACRVLPLAREEWEALFRECGIDGRSRAEDISVSQY
                     VALSDAAGRARGASGP"
     gene            1140..2009
                     /gene="ispE"
                     /locus_tag="PLQ54_11085"
     CDS             1140..2009
                     /gene="ispE"
                     /locus_tag="PLQ54_11085"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOS93849.1"
                     /translation="MRDSVRLRSPAKVNLYLDVHSGRRDDGYHSLCTLVQAVGLFDQV
                     EIRRRPHGIRVRTDAPDVPADGTNLAARAAAAFLAACDVDEGVDICLLKAIPARAGLG
                     GGSSNAAATLVGCRELFGLDVTDAELCRLAAGLGADVPFFVRGGTQVGHSRGEILEAV
                     EPPLVGWVVIAKGSEGASTPDVYAEFDRAPERAPMTIDVALERIRQRQWSALWNGLQG
                     AALRVAPAIRETRARLAEVSLGPVVVAGSGSSVFAVFSDAASAQAACARVAPAVAFSR
                     ACPMIPRGVEVVG"
     gene            2002..>2607
                     /locus_tag="PLQ54_11090"
     CDS             2002..>2607
                     /locus_tag="PLQ54_11090"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOS93850.1"
                     /translation="MASPRVGAVLLAGGRASAGLAAAGGSENKALADIHGWPMVRYVW
                     EALARAETVAHRVLVSDERVRQALGVREGEWCPCSGGPVDNIVSGAEALPDTVTHVLI
                     VSCDTPLVRPADLDGFVGASLDLGVSLCYSVLDQRTCTGAFAGVRKTWVRVREGTLAG
                     GNLALVSRPFLVDHQDRVRQAFELRKSPFALAMRLGITFALS"
BASE COUNT          332 a          917 c          914 g          444 t
ORIGIN      
        1 gcgcccaatg cacccgacgg tagctccacc ggccgcacgt ccctcgggtt gccggcgggc
       61 cgcggcgtcg tggcggtcga cccgagtgtg gtcccctacg gcacccgtat gtggatcgag
      121 ggctacggct gggcggtggc cggtgatacc ggtggcgcca tcaagggccg gaagatcgac
      181 ctgtgtttcg acgactacgg ctatgcggcc cggttcggca cgagaaccgt gaccgtgcgc
      241 ttcttcgagt agccgggtgc ccacgtgacg cgcccccccg agcctcgtca tgatgccatc
      301 cagctccgca ccgccgcccc tgcgccgccc aagaggtcgc tctcccagaa cttcctcgtc
      361 gaccgcaaca cggccacggc catcgtgacc gccgcggcga tccagccgag cgagtccgtt
      421 ctcgagatcg gtcccggccg cggggccctg accttcgggc tggcggaagc cgccgcgcga
      481 ctggtggccg tcgagcgcga cgacgccctc gccccatggc tggccgagca gcttgcctcc
      541 catggcgacc gcgttcgcat catccacgcc gatgtgctcg aagccgacct ggcagctctt
      601 ctcgcgcctc cggggccttg ggtggtggtc gggaaccttc catatgccat caccacgccc
      661 attctgctga agctgctcca gcatgcacgg cgcttcgatc gggccatcgt catggtccag
      721 cgcgaggtcg cccaacgctg gtgtgcgggg cccggctccc gaacctacgg cgccatcacc
      781 gtgtggtgcc agtaccattg ctcatgccgc gtgctccgaa aggtgcgccc ctcctgcttc
      841 cggccgtcgc cttcggtcga gtccgcgctc gtcgagcttc ggccgcactc cgacgccctt
      901 ccccgcgccc gggacgaggc gctgctgcag caggtgaccc gggccgcctt cggccagcgg
      961 cgcaagcggg ccgccacatc cctggccgct tgccgggttc ttcctctggc ccgggaggaa
     1021 tgggaggccc tcttccgcga atgcggcatt gacgggaggt ccagagccga ggacatttcg
     1081 gtgtcacagt acgttgcgct gagcgacgcg gcgggccgag cacggggggc gagtgggcca
     1141 tgagggactc ggttcggctg cgatcccccg ccaaggtcaa cctctacctc gacgtccact
     1201 caggaaggcg tgacgacgga taccacagcc tgtgtaccct ggtccaggcc gttgggctgt
     1261 tcgaccaggt ggagatccgc cgccggcccc acggcatccg tgtgcggacg gacgcgcccg
     1321 atgtccccgc cgatggcacg aacctcgccg cccgcgccgc ggccgcgttc ctggcggcct
     1381 gtgacgtgga tgagggggtc gacatctgct tgctgaaggc gatccccgcg cgggccgggt
     1441 tgggtggcgg gagctcgaac gccgccgcca cactggtggg ctgccgcgag ctattcggcc
     1501 tcgacgtgac cgacgcagag ttgtgccgcc tcgcggccgg cctgggagcc gacgtgccgt
     1561 tcttcgtccg cgggggcaca caggtgggcc acagccgcgg cgagatactc gaggccgtcg
     1621 agccgccgct ggtgggttgg gtcgtcatcg ccaagggctc cgagggtgca tcgacaccgg
     1681 acgtctatgc cgagttcgac cgggctcccg agcgcgcacc gatgaccatc gatgtggcgc
     1741 tggaacggat ccggcagcga cagtggagtg ccctgtggaa cgggttgcag ggcgcggccc
     1801 tgcgcgtggc gccggccatc cgggagacgc gggcccggct ggctgaggtc tccctcgggc
     1861 ccgtcgtcgt ggccggatcc ggctccagcg tgttcgcggt cttctcggac gccgcctcgg
     1921 ctcaggcggc gtgcgctcgc gtggctccgg cagtggcgtt ttcccgcgcg tgcccgatga
     1981 ttccccgggg ggtggaagtc gttggctagc ccacgagtgg gcgcggtgtt gctcgccggg
     2041 ggtcgtgcat cggccggcct ggcggcggcc ggaggctctg agaacaaggc actggccgac
     2101 atccatggtt ggcccatggt ccgctacgtg tgggaggctc tggcccgagc cgagaccgtg
     2161 gcccaccggg tgctggtctc cgatgagcgg gtccgacagg ccttgggcgt ccgcgagggc
     2221 gagtggtgcc cctgcagcgg tggtccagtc gacaatatcg tgtccggtgc cgaggccctg
     2281 cccgacacgg tcacccacgt gctcatcgtc agctgcgata cccctctggt ccgtcctgcc
     2341 gacctcgacg ggttcgtcgg ggcttctctt gacctcggag tgtcgctctg ctattctgtc
     2401 ctggatcagc gtacctgtac cggcgccttc gcgggtgtcc ggaagacatg ggtgcgcgtc
     2461 cgggaaggca ccctggccgg cggcaacctc gcgttggtga gccggccttt tctggtcgac
     2521 catcaggacc gcgtccgtca ggcatttgag ctgcgcaaga gcccgttcgc cctcgcgatg
     2581 cgcctcggga ttaccttcgc gctcagc
//
LOCUS       DAOSPL010000591         2606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39480_length_2606_cov_2.938064, whole genome shotgun sequence.
ACCESSION   DAOSPL010000591 DAOSPL010000000
VERSION     DAOSPL010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39480_length_2606_cov_2.938064"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PLQ54_11095"
     CDS             complement(<1..578)
                     /locus_tag="PLQ54_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93851.1"
                     /translation="MAPVALLAALWPCGLAVQADFAAIDPRPGMEIRFARRSSEVSSS
                     PFATLTSFEVHGADEDAGVIQAMAALGVKHVVMHNIHGSLSDAHDPRLNAWLDGCARE
                     GIDVRCILASPDPELWRVALTNYGDRIHHWCYLNEPNAPGADNDHSRPVVRPEQYVEG
                     LRQIRAVLKEVRPDAWLWGPEEAMLQHMEESP"
     gene            complement(598..1272)
                     /locus_tag="PLQ54_11100"
     CDS             complement(598..1272)
                     /locus_tag="PLQ54_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HOS93852.1"
                     /translation="MPSAGLELVEHLWEGYALSLPELVEGIEAPWDVVARAPDLIAAA
                     ARRDVQGDVHPSAIIEGDCLIERGASVGPCAYICGPTIIRSGASVRHGAYIRGHCLLE
                     PGALVGHDTEVKNAVFLSDAKAPHFAYVGDSVLGAHCNLGAGTKLANVRVVPGNVVVR
                     IGDERYDTGLRKLGALVGNRCKVGCNVVLNPGTVLEMDCVVYSCVSVRGHHVRGSIVK
                     GGSEPR"
     gene            complement(1276..>2606)
                     /gene="glmM"
                     /locus_tag="PLQ54_11105"
     CDS             complement(1276..>2606)
                     /gene="glmM"
                     /locus_tag="PLQ54_11105"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOS93853.1"
                     /translation="ANDTLLPEDVLRLGYAYGRYLAAQGDRRPLVIGRDPRLSSTMFC
                     SAFKAGVCSAGIDVLDSGVLSSPALAYLAGHLEVCAGAMISASHNPAHHNGVKLFDCH
                     GQKLAAAAELEIEELANSPDRSPRVDATEVGCVGHLSDPLSHYLDHLRASVSGVSLAG
                     LKVVFDSANGAASGYGAQLIRELGASVIHCNHEPNGININTRCGATYPQGIRGAVLTN
                     AADIGICVDGDADRVALVDEHGTVLDGDHIKWVWASHEHARGRLRNPTVVGTVMSNLG
                     LEIALKQIGCGLVRTLVGDRYVYERMIEMGALVGGEPSGHIIFSDYARTGDGLLSALQ
                     ILRVMHETGAKLSRLAGTMRKTPQVLRSVPFDTGTEWRTPAIVRAIADWEDRLGETGR
                     IVVRESGTEPLLRVMCECTDPATADRVVEDLLGLFAKHPGAISAAQKARE"
BASE COUNT          461 a          890 c          836 g          419 t
ORIGIN      
        1 cacgggctct cctccatgtg ctggagcatg gcctcctcgg gcccccagag ccaggcgtcg
       61 ggccgtacct ccttcagaac ggcgcggatc tgccgcaatc cctcaacgta ctgctcgggc
      121 cggaccaccg gcctactatg gtcattgtcg gcgccgggcg cgttgggctc gttgaggtag
      181 caccagtgat ggatgcggtc gccgtagttg gtcagtgcca cgcgccacag ctcggggtcg
      241 ggcgatgcga ggatacagcg gacgtcgatg ccctcacgcg cgcagccgtc cagccaggca
      301 ttcagccgcg gatcgtgggc gtcgctgagt gaaccgtgga tgttgtgcat cacgacatgc
      361 ttaacaccca gtgccgccat ggcctggatg acgccggcgt cctcgtccgc gccatggacc
      421 tcgaacgacg tgagggtggc gaacggcgat gagctgacct cagaactgcg gcgcgcgaaa
      481 cggatctcca tgcccggtcg cggatcgatc gctgcaaagt cggcctgcac tgccaggccg
      541 cagggccata gagcagcgag cagagcaaca ggggccatgg cgtcaccttc cggcgactca
      601 gcgcggctcg gatccgcctt tcacgatgct cccacggacg tggtgccccc ggaccgagac
      661 gcacgagtag accacacagt ccatctcgag taccgtcccg gggttcagca cgacgttgca
      721 gccgaccttg catcggttgc ccacgagcgc tccgagcttg cggagtccgg tatcatatcg
      781 ctcgtcgccg atgcgcacca ccacgttccc tgggaccaca cgcacattgg ccagtttggt
      841 ccccgctccg aggttgcagt gggcacccag gacgctgtcg ccgacgtagg cgaagtgggg
      901 ggccttggcg tcggaaagga acacggcatt cttgacctcg gtgtcatgcc ccaccagggc
      961 tcctggctca agcaggcaat ggccccgaat gtaggcgccg tgccggaccg aagcacccga
     1021 gcggatgatg gtcggcccgc agatataggc gcacgggccg accgatgcgc ctctctcgat
     1081 cagacagtca ccctcgatga tggctgatgg gtggacatcg ccctggacgt cgcggcgggc
     1141 cgccgccgca atcaggtccg gggcccgcgc aacgacatcc cagggcgcct cgatcccctc
     1201 gacgagctcg gggaggctga gcgcgtagcc ctcccagagg tgctcgacaa gctccagacc
     1261 cgcgctcggc atggctcact ctctcgcctt ctgggcggcc gagatggcgc cggggtgctt
     1321 ggcgaacagg cccagcaggt cctcaacaac ccggtccgcg gtagcgggat cggtgcactc
     1381 gcacatgacg cgcagtagcg gctcggtgcc cgactcgcgg accacgatgc gccccgtctc
     1441 ccccaaccga tcctcccagt cggcaatcgc gcggacgatg gcgggggtcc gccactcggt
     1501 gccagtgtca aacggcacac tacggagcac ctggggcgtc ttccgcatgg tgccagcgag
     1561 ccgcgagagc ttcgcacctg tctcgtgcat gacccgaagg atctgcaggg ccgaaagcag
     1621 gccatcaccc gtgcgggcat agtcgctgaa gatgatatga ccactgggct ctccgccgac
     1681 caaagcaccc atctcgatca tgcgctcgta gacgtaccga tcgccgacca gggtgcggac
     1741 cagcccacat ccgatctgct tcagggcaat ctcgaggccg agattgctca tcactgtacc
     1801 cacaaccgtg ggattccgga ggcgcccccg ggcgtgctcg tggcttgccc agacccactt
     1861 gatgtggtca ccatcgagca ccgtcccgtg ctcgtccacc aaggccacgc ggtcggcgtc
     1921 gccgtcgacg cagatgccga tgtcggcggc gtttgtcagc accgcgcctc ggatcccctg
     1981 ggggtacgtg gcaccgcagc gcgtgttgat gttgattcca ttgggctcgt gattgcagtg
     2041 gatcaccgac gcgcccagct cgcggatgag ctgggcacca tagcctgacg ccgcaccatt
     2101 cgccgagtcg aagaccactt tgagtcccgc gagggacacc ccacttaccg atgcccgaag
     2161 gtgatccaga tagtgcgaga gcggatcact caggtggccc acacacccaa cctcggtcgc
     2221 gtcaacccgg ggggatcggt ccggcgagtt cgccagctcc tcgatctcca gctcagcagc
     2281 cgccgccagc ttctggccgt ggcagtcgaa gagcttcacg ccattgtggt gagcggggtt
     2341 gtgcgacgcc gagatcatgg ctcccgcgca cacctcgagg tgaccggcca ggtacgccag
     2401 ggccggcgac gacagcacgc cggaatccag gacgtcgatg ccggcagagc atacgccggc
     2461 cttgaatgcc gagcagaaca tggtcgagga cagtctcggg tcccggccga tgaccagcgg
     2521 acgccggtca ccttgcgcgg ccaagtacct cccataggca taccccaagc gaagaacgtc
     2581 ctcgggaagg agtgtgtcgt tggcta
//
LOCUS       DAOSPL010000592         2606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39484_length_2606_cov_2.466876, whole genome shotgun sequence.
ACCESSION   DAOSPL010000592 DAOSPL010000000
VERSION     DAOSPL010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39484_length_2606_cov_2.466876"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PLQ54_11110"
     CDS             complement(<1..402)
                     /locus_tag="PLQ54_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93854.1"
                     /translation="MRYRARPSPPPRHTLALAMVLAVSAAGGQGEPRGGKDTPTRAEL
                     LTRVEAQEPAIARARAELQGTSPEADDCLALAQLAVIEARMAVESPYWSLYGAEYVDG
                     RLSSAAAALDLARDHKPLPIVPGELLERAYFA"
     gene            566..1822
                     /locus_tag="PLQ54_11115"
     CDS             566..1822
                     /locus_tag="PLQ54_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93855.1"
                     /translation="MGMLAKHHSIVRIAGVCAVVALPLGAMGCSRAAVRPTEAISEVA
                     IPIAEGQGAEGWNAYVLAVRQVRDENTIRTLYGDAQRLAPVLDASDQGRLGSPRLSPA
                     IAKVLASNAAALRLIHEGLEKPCRVPNLELRGPSDVGAAGGMKALGVLLLLEGRQREL
                     ERQPARAFESYQAVIRLGQQLRATAVGALLVSTRSIVEDGCREMRTSLRTGVPMTDGQ
                     IEQNISFIDKTIAAMPSIASLLTRERDAALGWVQVAVPQKSEVLADAREQGGPDYRPD
                     LQVITRQIREYYAAAMRSAALPCAEALANGVPRPSDPIAAAVCDDYRTLVYGEHRLRA
                     ALLATRLCFAVRRYQETNRFAPRSLEAMVPSFLSAVPADPISGEPFRIRSEGERYLVY
                     SVGPDGQDDGGKVMAVAYGQKGDFIY"
     gene            complement(1852..2301)
                     /gene="fliS"
                     /locus_tag="PLQ54_11120"
     CDS             complement(1852..2301)
                     /gene="fliS"
                     /locus_tag="PLQ54_11120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOS93856.1"
                     /translation="MSVPKAYQNYRRTAVETASRGRLILLLYDGLLRFLEQARTELAE
                     RKPKEAHFALIKAQNVLSELMASLDLDQGGEIARNLLAIYEFMFMQLVDANFRKSAEP
                     VDEVVDLVRTLREAWDRAVSEETADRNRQALEGPEPPGENGGFSVVS"
     gene            complement(2366..>2606)
                     /gene="fliD"
                     /locus_tag="PLQ54_11125"
     CDS             complement(2366..>2606)
                     /gene="fliD"
                     /locus_tag="PLQ54_11125"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOS93857.1"
                     /translation="ALKSDGVLGYRVKTDGTIEKDIDRVEDSITAYEARLKAREEALN
                     RQFTAMESMLSQLQSQGDYVLSTLSSASKQSSGSS"
BASE COUNT          405 a          865 c          882 g          454 t
ORIGIN      
        1 ggcgaagtag gcacgttcga gcaactcgcc cggcacgata ggcagcggct tgtggtcgcg
       61 tgccagatcc agtgcggccg cggccgacga gaggcgccca tcgacgtatt cggcgccata
      121 gaggctccag taggggctct cgacggccat cctcgcctcg atgaccgcaa gctgggccag
      181 ggccaagcag tcatcggcct ccggactggt tccctgcagt tcggcccggg cccgggcgat
      241 cgcaggctct tgggcctcga cgcgtgtcag cagctcggcc cgcgttggtg tatccttgcc
      301 cccacggggt tcgccttggc ccccggccgc cgagacggcc aggaccatgg ccagggcgag
      361 ggtgtgacgt ggcggaggcg agggacgcgc acggtatcgc atgggcaggc tccggtcttg
      421 ggctgtgtgg agagcgttcg gtgcgggcaa gcgcccgtcc tgcggatccg cgagcggagc
      481 cacaggaggc ccagaacaca ggacacacac agggcgaggc ggttgcgggg cgtgccgtgg
      541 attgccagtc aggagtgcga gtcacttggg aatgttggct aaacaccact ccatagtccg
      601 cattgccggt gtctgtgccg tcgtggccct tccgttgggt gcgatggggt gttcgcgggc
      661 cgccgtccgg ccgactgagg cgatctccga ggtcgccatc cccatcgccg agggccaggg
      721 cgccgaaggc tggaacgcct atgttttggc cgtccgacag gttcgggacg agaacaccat
      781 ccgcaccctg tacggtgacg cccagcgcct ggcgccggtc ctggatgcta gcgatcaggg
      841 ccgtctgggc tccccccggc tctctccggc catcgcgaag gtcttggcct cgaacgccgc
      901 ggcactccgc ctcatccacg agggcctcga gaagccctgc cgcgtgccga acctcgagct
      961 ccgcggaccg tccgatgtcg gggccgccgg tggcatgaag gcgctcgggg tgctgctcct
     1021 gcttgagggc cgacagcggg agctcgagcg gcaaccggcc cgcgccttcg agtcgtatca
     1081 ggccgtgatc cgcttggggc agcagttgcg ggccacggca gtgggcgcgt tgctggtgag
     1141 cacccgatcc atcgtggagg acggctgccg ggagatgagg accagtctgc ggacgggcgt
     1201 tcccatgacg gacggccaga tcgagcagaa catatcattc attgacaaga ccatcgcggc
     1261 aatgccgtcg atcgcgtcgc tcctgacgcg ggagagggac gccgcgctcg gctgggtcca
     1321 ggttgcggtc ccacagaagt ccgaggtgct ggccgatgct cgcgagcagg gcggacccga
     1381 ctaccgaccc gacctccagg tgatcacccg gcagattcgc gagtactatg ccgccgccat
     1441 gcggtcagcc gcactgccgt gcgctgaggc gctggcgaac ggagttccgc gaccttcgga
     1501 ccccattgcc gctgccgtgt gtgacgatta ccgcacgctc gtctacggtg agcaccggct
     1561 gcgtgcggcg ctgctggcca cgcggctgtg cttcgccgtg cgccgctacc aggagaccaa
     1621 ccgcttcgca ccccgcagcc tcgaggcgat ggttccgtcc ttcctgagtg ccgtcccggc
     1681 cgacccgatc agtggcgagc cgttccgtat ccgctccgag ggagagcgct atctggtgta
     1741 cagcgtgggc cccgacggcc aggatgacgg aggcaaggtt atggccgtcg cgtatggtca
     1801 gaagggtgac ttcatctact gacagcgggt caggcgttcc cgccgcccgg ttcacgagac
     1861 caccgagaac ccgccgttct cgcccggggg ctccgggccc tcaagtgcct gccgattgcg
     1921 gtcggcggtc tcctcgctca ccgcgcggtc ccacgcttct cgcagtgtgc gcaccaggtc
     1981 gaccacttcg tcgacgggct cggccgactt gcggaagttc gcgtcgacga gctgcatgaa
     2041 catgaactcg tagatcgcca ggagattccg cgcaatctca ccgccctgat ccagatcgag
     2101 tgaggccatc agctccgaca ggacattctg ggctttgatg agagcgaagt gtgcctcctt
     2161 gggcttccgt tccgcgagct cggtcctggc ctgctcgagg aaacggagca gtccatcgta
     2221 aagcaacaga atgagccggc cgcgcgacgc ggtttccacg gcggttcgcc ggtagttctg
     2281 gtaggccttc ggtacactca tcaacctcac ccgtcacatg cgttgtcacg agcccggccc
     2341 catgccagag cccggctcac cgtggctagc tactgcccga actctgtttg ctggcgctcg
     2401 agagcgtgct gagcacgtaa tccccctgcg attgaagctg tgagagcatc gactccatgg
     2461 ccgtgaactg ccggttcagg gcctcctcgc gagccttcag gcgggcttca tacgccgtga
     2521 tgctgtcctc gacgcgatca atgtccttct cgatggtgcc atcggtcttg acccggtagc
     2581 cgagcactcc gtcggacttg agggcg
//
LOCUS       DAOSPL010000593         2605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39501_length_2605_cov_2.749412, whole genome shotgun sequence.
ACCESSION   DAOSPL010000593 DAOSPL010000000
VERSION     DAOSPL010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39501_length_2605_cov_2.749412"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PLQ54_11130"
     CDS             <1..383
                     /locus_tag="PLQ54_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOS93858.1"
                     /translation="ATAIAIEADDPDACLVCDTFHLYRGGSGFRGIRQLRGSFLAVCH
                     FNDVPAFPPQERLADSDRIMPGDGILPLVRFVRDLWDIGFTGPLSLETFHREHWSRDP
                     RSVAREGIRKVRAVLDAARADGHA"
     gene            468..1907
                     /locus_tag="PLQ54_11135"
     CDS             468..1907
                     /locus_tag="PLQ54_11135"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOS93859.1"
                     /translation="MVDLAQKAVETALSRGATYADARICRYREQDLHTEDERVAGIGD
                     SESFGVGVRVIAQGAWGFAASATVTSEAVQAAAAQAVAIARASARVASKPVELVPEPP
                     RQSEFIGSCEVDPFAVPVEDKVGLLLELNRMLLSHAGISKAHAHMTLRSFTRTFVNSD
                     GSVLSSRIYTCGAGYTATAVDEHDSRSRSFEVPPMTRGYENIDAARLRENTTRVAEQA
                     VEHLKAPTIEDGRTTLILDPHNLALTIHESVGHATELDRALGMEESLAGSSFATPDKL
                     NKLQYGSPIVNFVAYNTLEHGLATWGFDDEGVECQTWHIVQDGVFRGYGASREVAGAI
                     GLPRANGTCRADSWASIPIVRQPNLCLMPGTEPLSLEELISDTDDGIYIEGMGSFSID
                     QKRLNFQFGGDAFWRIKSGRLAGMLKNVTYQSITPEFWNSCDAICDERFWVKNGVLNC
                     GKGDPGQVAQMIHAAAPARFRNVTVRPAR"
     gene            1949..2134
                     /locus_tag="PLQ54_11140"
                     /pseudo
     CDS             1949..2134
                     /locus_tag="PLQ54_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
     gene            2305..>2605
                     /locus_tag="PLQ54_11145"
     CDS             2305..>2605
                     /locus_tag="PLQ54_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOS93860.1"
                     /translation="MRSAMACTLLCILVVVGAQAQDRDEDQAAVTQLVHQVVVACNAK
                     DATALESLMIEGAVFAMDAFGEAVVLDRDGLISGPQGLAAMPGAVLKEEVNEVDVA"
BASE COUNT          444 a          805 c          891 g          465 t
ORIGIN      
        1 tggctactgc catcgccatc gaggcggatg atcccgacgc atgcctggtg tgcgatacgt
       61 tccacctgta ccgaggcgga tcgggtttcc gcggcatcag gcagctgcgc gggtcattcc
      121 ttgccgtatg ccacttcaac gacgtgccgg catttccacc gcaggagcgg cttgcggatt
      181 ccgaccgcat tatgccggga gacggcatcc tccctctggt gcggttcgtc cgcgacctgt
      241 gggacatcgg tttcacgggg ccgctgtcgc tcgagacgtt ccatcgcgag cactggtccc
      301 gggatccacg cagcgtggcg cgcgaaggta ttcgcaaggt tcgggcggtc ctggacgcgg
      361 cgcgcgccga cggacatgca tgagccgctg caggtccggc ccggccgcgc acgaagtagg
      421 tacgacgcag acatgggccg ccaccggcgg ccctcagggg gagaccgatg gtagaccttg
      481 cccagaaagc cgtggagacc gccctcagcc gtggtgccac ctatgccgac gcccggatat
      541 gccgataccg ggagcaagac ctccacacgg aagacgagcg ggttgccggc atcggcgact
      601 cggagagctt cggcgtgggg gtccgcgtga tcgcgcaggg cgcatggggt ttcgcggcgt
      661 ccgccacggt cacgtctgag gcggtgcagg ctgcggcggc gcaggcggtc gcgatcgctc
      721 gagccagtgc ccgggtggcg agcaagccgg tggagctggt accggagccg ccccgccagt
      781 ccgagttcat tgggtcgtgc gaggtggacc ccttcgccgt gcccgtcgag gacaaggtgg
      841 gtctgctact ggaactcaac aggatgctcc tatcgcacgc cggcatctcg aaggcacatg
      901 cccacatgac cttgcggtcg ttcacgcgta ccttcgtgaa ctccgacggg tctgtgctct
      961 cgagccggat ctacacctgt ggggccgggt atacggccac ggcggtggac gagcacgatt
     1021 cgagatcccg cagcttcgag gtgcccccga tgactcgagg gtatgagaac atcgatgccg
     1081 cgaggcttcg ggagaacacg acgcgggtgg ctgaacaggc ggtggagcac cttaaggcgc
     1141 cgacgatcga ggatgggcga acgacactga tcctcgaccc gcataacctg gcactcacca
     1201 tccatgagtc ggtggggcat gcgacggaac tggatcgggc cctgggcatg gaggagagtc
     1261 ttgccggcag cagtttcgcg acacccgaca agctgaacaa gctgcagtat ggctcgccga
     1321 tcgtcaactt cgtggcgtac aacaccctcg agcatggtct tgcaacctgg ggcttcgacg
     1381 acgagggagt ggagtgccag acgtggcaca tcgtccagga tggtgtgttt cgaggctacg
     1441 gcgccagccg tgaagtggcg ggcgccattg gtctacctcg agcgaatggg acctgtcgcg
     1501 ccgactcgtg ggcctcgatc cccatcgttc ggcagcccaa cctgtgcctc atgcccggca
     1561 cagagccgct gtcactcgag gagctcatca gcgacaccga tgacggcatc tacattgagg
     1621 gaatgggcag cttctcgatt gaccagaagc gcctgaactt ccagtttggt ggcgacgcgt
     1681 tctggcgcat caagagcggc cgtttggccg gcatgctcaa gaacgtgacc taccagagca
     1741 tcacacccga gttctggaac tcctgcgatg ccatctgtga tgagcgtttc tgggtcaaga
     1801 acggcgtctt gaactgcggc aagggcgacc cgggacaggt cgctcagatg atccatgcgg
     1861 cagccccggc ccgtttccga aacgtgaccg ttcgtcccgc caggtagggg agcaccgggg
     1921 ttggcccgct gatgacggag gcgcgggctt gaacgtcgag agagtcattc gcgtcatcgc
     1981 tgggactttc gtgttggcca gtgtcgctct gggctactgg gtgagcccct actggttcct
     2041 gttcacggcc ctggttggtt tgaacctggt ccagtcgggt ttcaccaact ggtgtctgat
     2101 ggagaccata ctcgtgcggg cgggcctgcc gcgcgccggc gatgccccga ggactgcccg
     2161 gttcacggtc aagcgcaagc ccaaacgggc agctccgagg cccaaggcgg gcaagcgagc
     2221 atcctgatgg cccgacgtcg cgactcgagg cgctcacggg tgtccgatcg ccgggtcacc
     2281 gaacagaagg gggtctgctg tcctatgcgt tccgccatgg catgtacgct tctgtgcata
     2341 ctggtcgtcg tcggcgctca ggctcaggat cgggacgagg atcaggcggc cgtcacccag
     2401 ctcgtccatc aggtagtggt tgcctgcaat gcgaaggacg ccacggcgct cgagtcgctg
     2461 atgattgagg gcgccgtttt cgccatggac gccttcggtg aggccgtggt gctggaccgc
     2521 gacgggctga tctcgggccc ccaggggctc gccgccatgc ccggtgccgt cctcaaggag
     2581 gaggtcaacg aggtcgacgt cgcct
//
LOCUS       DAOSPL010000594         2601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39589_length_2601_cov_2.593873, whole genome shotgun sequence.
ACCESSION   DAOSPL010000594 DAOSPL010000000
VERSION     DAOSPL010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39589_length_2601_cov_2.593873"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PLQ54_11150"
     CDS             complement(<1..552)
                     /locus_tag="PLQ54_11150"
                     /inference="COORDINATES: protein motif:HMM:NF040059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93861.1"
                     /translation="MAIEPGQVVVFTGGGDLRAVLQSLSAELTLRGRVLLTTTTQMAP
                     ALGEDDPPPVLASRYDDLLAGLETMLASQRRAIATRGVLAGGLLRGVPRGWIDRLCEA
                     NIADYILVLGDDARGRPLKVAGADDPVIPGSASVVMPCAAVSVVDRPLDDTVAYRPEV
                     LAARLELQFRQRLTPRHIARALLG"
     gene            747..2399
                     /gene="ilvD"
                     /locus_tag="PLQ54_11155"
     CDS             747..2399
                     /gene="ilvD"
                     /locus_tag="PLQ54_11155"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOS93862.1"
                     /translation="MGTRMLEAPEGALVRSLYRSMGYGDPDFAKPVIAVVNSASTVCP
                     GHYHLPALALQARQGIAEGGGMPVEFGTIGPCDGIAQGHDGMRYILPCRDLIAADIEM
                     MARAHLIEGLVLVGSCDKIIPGMLMAAARLDLPAIVVVGGPMQPGCVKGKHCDSSSAQ
                     IAYAEYRAGSITREQLRQAEIDATPGPGSCSMLGTANTMCCIAEALGMALPLSATIPA
                     IDAARLYSARESGRQAVELVRRGLTARRIMTRGAIENAIRLCLAIGGSTNAALHMPAI
                     AAESGIDAPLQLFDDLGRTTPTLARILPSSEWDMVDFHGAGGVAGVMHELMPLLDSEA
                     LTVTGRTVRETFADTKPVVGPVVRPAAEPFARTGGLAVLRGSLAPGTAVARPAAVLEH
                     MRRFRGPARVLESEEAACEAILTGKIRPGDVMVIRNEGPRGGPGMREMFTPLKALEGV
                     GLLDAVALVTDGRFSGSNKGLFIGHVVPEAADRGPIAAVRDGDAIEIDIEARRVDLDV
                     PSDEVVARLSALPPFEPPVKSGFLGIYSQLVRPASEGAMLRA"
BASE COUNT          366 a          831 c          992 g          412 t
ORIGIN      
        1 accgaggaga gcccgggcga tgtgccgggg cgtgagccgc tggcggaact gcagttcgag
       61 ccgggcggcc aggacctccg ggcggtaggc gacggtgtcg tcgaggggtc ggtcgaccac
      121 gctgacggcc gcgcagggca tgacgacact cgccgacccg ggtattacgg gatcgtcggc
      181 gcccgcgacc ttgaggggcc ggcctcgggc gtcgtcgccc agcaccagga tgtagtccgc
      241 gatgttggcc tcgcacaagc ggtcgatcca cccacggggc acgccgcgga ggagacctcc
      301 ggccaggacg cctcgggtag cgatggcccg gcgttggctg gcgagcatgg tctcgaggcc
      361 ggccaggagg tcatcgtagc ggctcgccag caccggtggc gggtcgtcct cgccgagggc
      421 cggggccatc tgggtggtgg tggtcagcag aacacgaccg cggagggtga gctcggcgga
      481 caaggactgg agcacggctc tgaggtcgcc gcctcccgtg aacaccacca cttggcccgg
      541 ttcgatcgcc aaggcttcgc ccaccgtccg caccggggcc ggcgtgggtg cccttaacgc
      601 ctctcgcatt ggatcgctcc gttctcggcg ttcccgccca gagtgcatgg ggcggttcgg
      661 cgctgggcgc actcatcctg cgaggccagc gaaaggcgct cggcgcgctg ttcgagaaca
      721 gtctccccgc aggggaggaa ggcttgatgg gaacgcgcat gctggaggcc cccgaggggg
      781 ctctcgtccg ctcactatac cgctcgatgg gctacgggga tccggatttc gccaagccgg
      841 tgatcgcggt ggtcaactcg gcgagcacgg tgtgtcctgg ccactaccat cttccggctc
      901 tggcgcttca ggccagacag gggatcgccg agggtggtgg catgcccgtg gagttcggca
      961 ccatcgggcc gtgcgacggg attgcgcagg gccatgacgg gatgcgctac atcctgccgt
     1021 gccgggacct gatcgcggcc gacattgaga tgatggcccg ggcgcacctg atcgagggac
     1081 tcgtgctggt gggctcctgc gacaagatca tcccggggat gctcatggcg gccgcccgac
     1141 tggacctgcc ggccatcgtc gtcgtcggcg gccccatgca gcccggttgc gtgaagggga
     1201 agcactgcga ctcatcatcg gcgcagatcg cgtatgccga gtaccgggcc gggtcgatca
     1261 cgcgcgagca gctccggcag gcggagatcg atgcgacgcc cggtcccgga tcgtgttcga
     1321 tgctcggcac ggcgaacacc atgtgttgca tcgccgaggc tttggggatg gcgctgccgc
     1381 tgtcggccac gattccggcc atcgatgcgg cgcgtctcta ctcggcccgc gagagcggac
     1441 gccaggcagt ggagctggtg cgccgaggcc tcacggcccg ccgcatcatg acgcggggcg
     1501 ccatcgagaa cgcgatccgg ctgtgcctgg ccatcggagg atccacgaat gccgcgttgc
     1561 acatgccggc catcgccgcc gagtccggga ttgacgcgcc cctgcagctc ttcgacgacc
     1621 tgggtcggac cacgccgacg ttggcgcgta tcctcccctc cagcgagtgg gatatggtgg
     1681 acttccacgg cgcgggcggg gtggccggcg tgatgcatga actcatgccc ctcctcgatt
     1741 cagaggcact cacggtgacg gggcgtaccg tgagggagac cttcgccgac acgaagcccg
     1801 tggttggtcc tgtcgtgcgg ccggcggcag agccattcgc tcggaccggc ggattggcgg
     1861 tgctacgcgg ctcgttggct ccgggcacgg ccgtggctcg gcccgcggcc gttctcgagc
     1921 acatgaggcg cttccgcggg ccggcgcggg tgctggagag tgaggaagcg gcttgcgagg
     1981 ctattctgac gggcaagatc cggccgggcg acgtgatggt gatccgtaac gaggggcccc
     2041 ggggtggtcc gggaatgcgg gagatgttca ctccactcaa ggctctggag ggcgtgggcc
     2101 tgctggacgc cgtggcgctg gtgaccgacg ggcgcttctc ggggtccaac aagggtctgt
     2161 tcatcggcca tgtggttccc gaggcggcgg atcgcggccc catcgcggcg gtgcgagatg
     2221 gcgacgcgat tgagatcgac atcgaggcgc ggcgcgtgga tctggatgtg cccagcgacg
     2281 aggtggtggc acggctttcg gcgttgccgc cgttcgagcc gccggtcaag agcggtttcc
     2341 tgggtatcta ctcgcagctg gtgcgaccgg cttcggaggg cgcaatgctg cgggcctgag
     2401 gcggcggccc agagcccccc cggaggcgag ggctccaggg caggggcgga gacttgggcc
     2461 cgggcggcgc ccgggagcct caggttgccg cccatggggc tccgggtcct tcgaccggct
     2521 ccgcacaggg cattggcggt aacatggggc ccttggccgg gcggctgggt ctcgcgaagg
     2581 tcgcgcggaa cgcccgcctg g
//
LOCUS       DAOSPL010000595         2600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39608_length_2600_cov_2.761100, whole genome shotgun sequence.
ACCESSION   DAOSPL010000595 DAOSPL010000000
VERSION     DAOSPL010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39608_length_2600_cov_2.761100"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLQ54_11160"
     CDS             complement(<1..358)
                     /locus_tag="PLQ54_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOS93863.1"
                     /translation="MRETGCSVLEFAASLESGRGYEGQITGRTVIPARPARYADLERP
                     LSPAIQAVLDALGIRQLYSHQAQAIDRIRRGSNVAVVTSTASGKTLCYNIPVMERLLE
                     SRGSRAAYIFPTKALAQ"
     gene            complement(657..2081)
                     /locus_tag="PLQ54_11165"
     CDS             complement(657..2081)
                     /locus_tag="PLQ54_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOS93864.1"
                     /translation="MTKRRASIVIIDDDALVRRTLATGLRALGYGVSEATGGREGLSL
                     VGSIEPDLVLCDLGMPGMDGLAVLDEMGRQHPQVPVVLVTADDRIDTAVEAIRRGAFH
                     YVPKPVDMRSLAVTVQRALDARALARRVRTLERELAASGSFEALIGTSPVFRQACQTA
                     VKVAATSVTVLITGESGTGKELVAAAIHRASPRRSGPYVTVNCTALPAGLLESELFGH
                     EKGAFTDASEARPGQFERAHGGTILLDEIGDMSVELQPKILRVLESREVERLGSARPT
                     PVDARIIAATNQDLRQAVAEGRFRADLYYRLNVVTVELPALRDRREDIPLLATHFLQR
                     AATRYQVERTGFSPEAIAALEAYPWPGNVRELQNAVERAAILGSQPVIGLDDLPDELR
                     SGASLRPELTPTVATPHFPVSEEPVLTLAEVERRHIARVLELNGGNQSKTARDLGLKR
                     GTLLSRMQKLGLTGASGSVDLSTQ"
     assembly_gap    2359..2368
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2369..>2600)
                     /locus_tag="PLQ54_11170"
     CDS             complement(<2369..>2600)
                     /locus_tag="PLQ54_11170"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOS93865.1"
                     /translation="VFAYIIIPFGLRFLYHFTTQSGIRWLLSVEKYLGFFLTLSLGLG
                     LVFQMPLVVAVLTKIGIVTPQFLASKRRYAIVV"
BASE COUNT          438 a          846 c          867 g          439 t
ORIGIN      
        1 cctgggcgag tgctttggtg gggaagatat aggccgcccg cgagccgcgg ctctcgagca
       61 ggcgctccat gaccggaatg ttgtagcaga gcgtcttgcc gctggctgtg gaggtaacca
      121 cggccacatt cgagccgcgc cgtatccggt cgatcgcctg ggcctggtgc gagtacagtt
      181 gccggatgcc cagcgcgtcc aggaccgcct ggatggcggg agagaggggc cgctccaagt
      241 cggcatagcg cgcgggccgc gccgggatca ccgtccggcc cgtgatctgg ccttcgtacc
      301 cccggccgct ctcgagggag gccgcgaact ccagcacact acacccggtc tcacgcattg
      361 aacacccaaa ctcccgtttg cttgttgtcc ggccccctgt gttttccctg cctggagcgc
      421 aaagagatcc cgggaatccg cgagggcccg gagccgtggc tccgggcccc ctcggggagg
      481 gtgaggactc tgtgcgttcg gcttcccgac ctcaaggccg tccaccgttc cgcacgcctt
      541 ggggggcgtc agtcctttgg gtgtcatcgc accatggctg caacggagtt gccaggggaa
      601 gtggcgcggc cgtggggtca ctggcgccat ggcggcaggt atctggtgat atggcatcac
      661 tgtgtcgaca aatcgacact gcccgacgcg ccggtcaggc ccagcttctg cattcgcgag
      721 agaagcgtcc cgcgcttcag gcctagatcg cgtgccgtct tcgactggtt gccgccgttg
      781 agctccagca cgcgggcgat gtggcgtctc tctacctcgg ctagagtgag aaccggctct
      841 tcagacaccg gaaaatgcgg ggtggcgact gtgggcgtga gctctgggcg caggctcgca
      901 ccagacctca gctcgtccgg cagatcgtcg agcccaatga ccggctgcga acccaggatg
      961 gccgcccgct cgaccgcgtt ctgcagctcg cgtacgttcc cgggccatgg gtacgcctcc
     1021 agggccgcaa tcgcctccgg cgagaagccc gtgcgttcga cctggtaacg agtcgcggcc
     1081 cgctggagga agtgggtggc cagtaaagga atgtcctcgc gtcgatctcg gagagcggga
     1141 agctcaacgg tcaccacgtt gagacggtag tacaggtccg cgcggaagcg tccctccgcc
     1201 acggcctgcc gaaggtcctg gttcgttgcc gcgatgattc gcgcgtccac gggtgtgggt
     1261 cgcgctgatc ccagacgctc gacctcgcgg ctctcaagca ccctcaggat cttgggctgg
     1321 agctcgaccg acatgtcgcc gatctcatcg agtaggatcg ttccgccgtg agcccgctcg
     1381 aactggcccg ggcgggcctc ggacgcgtcg gtgaacgcac ccttctcgtg accaaagagc
     1441 tcactctcga gaagccctgc cggtagggcc gtgcagttga cggtaacgta ggggccgctg
     1501 cggcgggggc tggcccgatg gatcgcggcc gccaccagct ccttgccggt cccgctctct
     1561 cccgtgatga gcacggtcac cgatgtggct gcgaccttga cggccgtctg acaggcttga
     1621 cggaagaccg gagatgtacc aatcagggct tcgaacgatc cgctcgcagc cagttcgcgc
     1681 tcgagggtgc gcacacggcg ggcgagcgcg cgtgcatcca gagctcgctg aacggtgacg
     1741 gccagcgagc gcatgtccac cggcttgggg acgtagtgga acgccccccg gcggatggcc
     1801 tcgacggccg tgtcgatgcg atcgtccgcc gtgaccagaa ccacgggtac ctgcggatgc
     1861 tggcggccca tctcgtccag cacggctagc ccatccatcc cgggcatgcc gagatcgcag
     1921 agaacgagat cgggctcgat gctccccacg agagacaggc cctcgcggcc cccggtagcc
     1981 tccgacacgc catagccgag agcccgaagc cccgtggcca gcgtgcgccg gaccagggca
     2041 tcgtcgtcga tgatcacgat cgacgcgcgg cgcttggtca tcgtcagctc agttgtccgt
     2101 atcagaggca ggcggcgtat ccgcgggctt gcggcttctc ttcggcgtcc gccgggcgcg
     2161 gacgcgtttc ggtttccgcc gcaggcgctg gatggggcgg gactcggagc cctctcgacc
     2221 gacgcaccgt gccacgatga tcgagacttc aaatagcact aacatgggga ccataacgat
     2281 cgacaggttc accacatcag gtgtcggcgt gatgatcgcg gcgatggctc gcgccggcta
     2341 tccgcaggcc ttctctgcnn nnnnnnnngc acgacgatcg cataccggcg cttcgaggcc
     2401 aggaactgcg gggttacgat gccgatcttg gtgagcaccg caacgacaag cggcatctgg
     2461 aacacgagcc cgagccccag cgagagggtg aggaagaagc ccaggtactt ctcaacgctc
     2521 agcagccatc ggatcccgga ctgggtcgtg aagtggtata ggaaccttag cccgaagggt
     2581 ataatgatat acgcaaaaac
//
LOCUS       DAOSPL010000596         2594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39704_length_2594_cov_2.961008, whole genome shotgun sequence.
ACCESSION   DAOSPL010000596 DAOSPL010000000
VERSION     DAOSPL010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39704_length_2594_cov_2.961008"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PLQ54_11175"
     CDS             <1..1011
                     /locus_tag="PLQ54_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOS93866.1"
                     /translation="VAKRRTQHEIARHQAQVVVGTHALIQEGVDFSRLGLAVIDEQHR
                     FGVMQRARLMTKGVHPHTLVMTATPIPRTLAMTLYGDLDVSVLDEMPPGRQPVSTRTM
                     GPRQAYALVRREVSRGRQAYVVCPLIEESETLDAEAAVALAERLRHGELAGIPLGLLH
                     GRLSPDEKDHVMGEFVAGRLSVLVSTTVIEVGVDVPNATVMVVENAERFGLAQLHQLR
                     GRIGRGQHASYCALVCAARTAAAHQRVAALCRTNDGFAIAEEDLRLRGPGEYFGTRQS
                     GLPDLQVADVIRDAELVAAAREDAFALVEADPGLMAPEHRALAWEVELRMRGRAHAAL
                     VG"
     gene            1024..1638
                     /gene="rsmD"
                     /locus_tag="PLQ54_11180"
     CDS             1024..1638
                     /gene="rsmD"
                     /locus_tag="PLQ54_11180"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOS93867.1"
                     /translation="MRAAGPSCRVVGGEYRGRRITIPKGLRLRPTSDRVKESLFSMLT
                     PYVAGARFLDLCAGIGTIGIEALSRGAVHAVFVEVSVPCARAIATNLHALGLEQRAEI
                     RVADAIREIRLRSEGEPFDIIFADPPYASDLPPRIVREVATHSNVLGEEGLVALEHDG
                     RWATGPGGWKCGTEDQDAALLNTLELVTERRYGDKMITILRRPS"
     gene            1641..2120
                     /gene="coaD"
                     /locus_tag="PLQ54_11185"
     CDS             1641..2120
                     /gene="coaD"
                     /locus_tag="PLQ54_11185"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOS93868.1"
                     /translation="MRTAVYPGSFDPVTMGHLDIARRAATMCDRLIVAVLRNPSKNPL
                     FTIGERVEMLEEVCRDMPNVEVDSFEGLLVDYACQRGAHILIKGLRAVSDFECELQQA
                     HINRRLDSRVDTVFLATEACYAYLSSSLVREVVMLGGSVAGLVPPGVEDRLIAKLRQ"
     gene            2159..>2594
                     /locus_tag="PLQ54_11190"
     CDS             2159..>2594
                     /locus_tag="PLQ54_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93869.1"
                     /translation="MEVHRLIEQLQDLVEHSSVFPWPGRRAVAYEEFYRLTEEIRRLL
                     PQELAQATAIMTTRDEVLAGATRQAQEIVEQAQREAQIRLEAVDRQVAEMVSQDAVAL
                     AAEERAREILAEAESQAAQIRAGADEYALALLNRLDAFVQRVQ"
BASE COUNT          411 a          777 c          969 g          437 t
ORIGIN      
        1 gtcgcgaagc ggcggaccca gcacgagatc gcgcggcacc aggcgcaggt cgtggtgggg
       61 actcatgcgc tgatccaaga gggggtcgat ttcagccggt tgggtctggc tgtgatcgac
      121 gaacaacacc ggttcggcgt catgcagcgg gcgcggctga tgaccaaggg ggtgcaccct
      181 catacgctcg tgatgacggc cacgcccatc cctcgcacac tggcgatgac cctctatggc
      241 gaccttgacg tctcggtcct cgacgagatg ccgccggggc ggcagccggt gtcgacaagg
      301 accatggggc cccgacaggc atatgccctc gttcggcggg aggtgtcccg aggccgtcag
      361 gcctatgtgg tctgcccgct gatcgaggag tcggagaccc tcgacgccga ggcggcggtc
      421 gccctggccg aacgcctacg gcacggcgag ttggcgggta tcccgctggg cttgcttcat
      481 ggccggctgt ccccggacga gaaggaccat gtgatggggg agttcgtagc cggtcgcctg
      541 tcggttctgg tgagcaccac cgtgattgag gtgggtgtgg acgtgcccaa cgccaccgtg
      601 atggtcgtgg agaacgctga gcggttcggg ctggcgcagc tccatcagtt gcgcgggcgc
      661 atcgggcggg ggcagcacgc gtcgtactgc gccttggtct gcgccgcgcg tacggcggcg
      721 gcgcatcaac gggtggctgc gctgtgccga accaatgatg gtttcgccat tgcggaggaa
      781 gacctccggc ttcgcggtcc cggcgagtac tttggcacga gacagagcgg gctgccggac
      841 ctccaggtcg ccgatgtgat tcgcgatgcc gaactcgttg ctgcagctcg cgaggacgcc
      901 ttcgcgctgg tcgaggccga tcctggcctc atggcgccgg agcaccgggc attggcgtgg
      961 gaggtcgagc tccggatgcg tggccgggcc cacgcggcgc tggtggggta ggggggatgg
     1021 gcgatgaggg cggcagggcc ctcgtgtcgc gtcgtcgggg gcgagtaccg ggggcggcgg
     1081 atcacgatac ccaaggggct gcgtctgcgc ccgacgagcg accgggtcaa ggaatcgctg
     1141 ttctcgatgt tgactccata cgtggccggc gcccgctttc tggacctgtg tgcgggcatc
     1201 ggaacgatcg gcattgaggc gctgagccgg ggtgcggtcc acgccgtatt cgtcgaggtg
     1261 tcagttccgt gcgcgcgagc cattgcgacg aacctgcacg ccttgggcct cgagcagcgg
     1321 gcggaaatac gcgtcgccga tgccatccgc gagatccggc ttcggtcaga gggcgagccg
     1381 ttcgacatca tctttgcgga cccaccgtac gcgtcggact tgcctccgcg gatcgtccgc
     1441 gaggttgcca cccactcgaa cgtgctggga gaggaagggt tggtcgcgct cgaacacgac
     1501 ggacgatggg cgacgggacc gggcgggtgg aagtgcggga cggaggacca agacgctgcg
     1561 ctcctgaata cgctggagtt ggtcactgag cggcgctatg gggacaagat gatcacgatc
     1621 ctgcggaggc cgagctagcc gtgcggactg ccgtctaccc gggtagcttc gacccggtga
     1681 ccatggggca cctggacatc gcgcgccggg cggccaccat gtgtgaccgc ttgatcgtgg
     1741 cggtccttcg gaatccgtct aagaacccac tgttcaccat cggcgaacgg gtggagatgc
     1801 tcgaggaagt gtgccgggac atgcccaacg tggaggtgga cagcttcgag gggctcttgg
     1861 tcgactacgc gtgccagcgg ggcgctcata tcctgatcaa gggtcttcgg gcggtgtcgg
     1921 acttcgagtg tgagctacag caggcccata tcaaccggcg gctcgactcg cgcgtcgaca
     1981 cggtgttcct ggcgacggag gcgtgctacg cgtatctgag ttcgagcctc gttcgcgaag
     2041 tggtaatgct gggggggagc gtcgccgggc tggtcccccc gggggtggag gaccggctga
     2101 tcgccaagtt gcggcagtag ggcgcagtgc ccacggccag cggccggtgg cgtgtgatat
     2161 ggaagtacac cgactcatcg agcagcttca ggacctggtg gaacactcct cggtgttccc
     2221 gtggcctgga cgccgcgcgg ttgcctatga ggagttctac cggttgaccg aagagattcg
     2281 gcgtctactg ccccaggagc tggcccaggc cacggcgatc atgaccacgc gcgatgaggt
     2341 cctggcgggc gccacccggc aggcacagga gatcgtcgaa caggcccagc gcgaggcgca
     2401 gatccgcctg gaggccgtgg atcggcaggt ggccgaaatg gtgagccagg atgcggtggc
     2461 gcttgcggcc gaagagcgtg ccagggagat cctggccgaa gccgagagcc aggccgctca
     2521 gatccgcgca ggggccgacg agtacgccct ggcccttctc aaccggctcg acgcgttcgt
     2581 tcagcgcgtg cagc
//
LOCUS       DAOSPL010000597         2592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39752_length_2592_cov_3.231376, whole genome shotgun sequence.
ACCESSION   DAOSPL010000597 DAOSPL010000000
VERSION     DAOSPL010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39752_length_2592_cov_3.231376"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PLQ54_11195"
     CDS             complement(<1..620)
                     /locus_tag="PLQ54_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93870.1"
                     /translation="MWYLRCLALVSAVAATMAGAQEGTPGMTKRPHILPRSECFFGLH
                     FDLHPNENDTELGAACTDENIGALLDRVDPDFIQWDCKGHRGYTGYPTRVGWPSPGIV
                     GDSLAVVRTATAARNIALYIHYSGVWDTRALEAHPDWGVVRADGTRDPNNTSVFGPYA
                     DQLLIPQLKEVVDRYDLDGMWVDGECWAAQLDWCEAARRQFTEETGL"
     gene            complement(750..2033)
                     /locus_tag="PLQ54_11200"
     CDS             complement(750..2033)
                     /locus_tag="PLQ54_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS93871.1"
                     /translation="MGRRRFLGAAAGTAIAAGMFGRASARGRRPVRVGVIGTGGRGTS
                     LLGILLGMEDVHVPAVCDISLPAAERARQMVADSGRPMPEAYTKGDHAYRGLVERDDL
                     DAVVIATPWSWHTPMAVDALQAGKYAAVEVPCALTLDECWDLVNTSEKTGIPCMMLEN
                     WSFRRDNLAVLNMIRQGLFGDMVHCHCAHSHDCIDHWFFDPQGNIRWGGEFLIRRNCD
                     QYPTHSLGPVLSWLDINCGDAFDSVTSTATESKGINAYFARKFGPDHPNAKRKYAQGD
                     IVTSVIRTHRGKSVVVNYDMQLPRPYDNRWMVQGTLGLYDEDRAVVYIEGRSPAYHEW
                     EPFAPYQEAFDHKWCQEAPTGAGHGGTDELELALFVKAVREGTQTPLDVYDSVTMSAL
                     VALSEKSIARHSEPVEFPDFTRGRWKTRKATFGVV"
     gene            complement(2117..>2592)
                     /locus_tag="PLQ54_11205"
     CDS             complement(2117..>2592)
                     /locus_tag="PLQ54_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93872.1"
                     /translation="RLAYILLVVAAACSSCDTGLSAFGALVMNDIYRAHINPAATDRQ
                     MLRVARWSLAAFAAAVASLAMRRVALLEVVFFLGAAKGAMLFPLVATLYWRRQNVWGF
                     AAGTLGGMAIGSCLFLLLRDSAANYRVLSTSLSVVMGAVLSIAVTHATGARTGRS"
BASE COUNT          424 a          831 c          908 g          429 t
ORIGIN      
        1 aggcccgttt cctcggtgaa ctgccgtctc gcggcctcac accagtcgag ttgcgcggcc
       61 cagcactcgc catccaccca catgccgtcc aggtcatacc ggtcgaccac ctccttgagt
      121 tgtgggatga gcagctggtc ggcatagggc ccgaagacac tggtgttgtt cgggtcgcga
      181 gtgccatcgg cgcgcacgac gccccaatcg ggatgcgcct ccagggctcg cgtgtcccac
      241 acgccggagt agtggatgta gagcgcgatg ttccgggccg cggtggccgt gcgcaccacg
      301 gccagcgagt cgcccacgat accgggcgac ggccagccca cccgggttgg gtagcccgta
      361 tagccgcgat ggcccttgca gtcccactgg atgaagtcgg gatcgacgcg atcgaggagc
      421 gcgccgatgt tctcatcggt acaggcggct ccgagctcgg tgtcgttctc gtttgggtgc
      481 aggtcgaagt gcaggccgaa gaagcattcg gacctgggga ggatgtgcgg gcgcttggtc
      541 atgccgggag ttccctcctg ggcgccggcc atggtcgcgg ccaccgcgga gaccagtgcg
      601 aggcagcgca ggtaccacat cgcggaccga cccccttatg tcgggggccg aagagggcgg
      661 agcccttggc ccccgaaggc tcagtgcaga tccggccgat gccggatgcc gggagaggtc
      721 tccgtggcct ctgcccgctt gtgacccttt cacaccaccc cgaatgttgc cttgcgggtc
      781 ttccaccgcc cccgggtgaa gtcggggaac tcgacgggct cgctgtgtcg cgcgatggac
      841 ttctccgaca gcgcgaccag ggcgctcatg gtgaccgagt catagacgtc gagcggcgtc
      901 tgagtgccct cgcgcacggc tttgacgaag agggccaact ccagctcgtc ggtccccccg
      961 tggccggcgc cggtgggcgc ttcctggcac cacttgtgat cgaacgcctc ctggtacggg
     1021 gcgaagggtt cccactcatg gtaggcaggg ctgcggccct cgatgtagac cacggcccgg
     1081 tcctcatcgt agagccccag agtgccctgg accatccatc ggttgtcgta tggccgcggg
     1141 agctgcatgt cgtagttgac cacgacggac ttgccccggt gggtgcggat aacagaggtc
     1201 acgatatcgc cctgggcgta cttgcgcttg gcgttgggat gatcggggcc gaactttcgg
     1261 gcgaagtagg cgttgatgcc cttcgactcg gtcgccgtgg atgtgaccga gtcgaaggca
     1321 tcgccgcagt tgatatcgag ccagctcagc acgggaccca ggctgtgagt tgggtactgg
     1381 tcgcagttcc gccggatgag gaactcgccg ccccaccgga tgtttccctg cgggtcgaag
     1441 aaccagtgat cgatgcagtc gtgcgagtgg gcacagtggc aatggaccat gtcgccgaag
     1501 aggccctgcc ggatcatgtt gagcacggcg aggttgtcgc gccggaagct ccagttctcg
     1561 agcatcatgc aggggatgcc cgtcttctcg gacgtgttga ccaggtccca gcactcatcg
     1621 agcgtcaggg cgcagggaac ctcgaccgcg gcgtactttc cggcttggag ggcgtcaacg
     1681 gccatgggtg tgtgccagct ccatggcgtg gcaatgacca cggcgtcgag gtcgtcgcgt
     1741 tcgacgaggc cgcggtaggc gtggtccccc ttggtgtagg cctcgggcat tggccgcccg
     1801 gagtcggcca ccatctggcg ggcgcgctcg gcggcaggca gcgagatgtc gcacaccgcg
     1861 ggcacgtgga cgtcctccat gcccagaagg atgccgagca ggcttgttcc gcgtccgccg
     1921 gtgccgatga cgccgacgcg gaccggtctc ctgccccggg cgctggcgcg gccgaacatt
     1981 ccggccgcga tggcggttcc ggcggcggcg ccgaggaagc gcctacgccc catgcatctc
     2041 tccatgcgaa atccccctca actctcgagt cagccgatga ttggctgagg agcgacacgg
     2101 gaccttcgtg gggcgcctac gatctgcccg tgcgggcccc agtcgcgtgg gtcaccgcaa
     2161 tggatagcac cgcgcccata acgacgctca gcgaggtgct caggacgcga tagttggctg
     2221 ccgagtccct gagcagcaga aagagacagg agccaatggc catgccgccg agcgtgccgg
     2281 cggcgaagcc ccacacgttc tggcggcgcc agtagagcgt ggccaccagc gggaagagca
     2341 ttgccccttt ggcggcgccg aggaagaaga cgacctcgag cagcgcgacg cgccgcatgg
     2401 ccagggaggc aacggccgcc gcgaacgccg ccaggctcca tcgggccact cgcagcatct
     2461 gccggtcggt agccgccggg ttgatatggg cgcggtagat gtcgttcatg accagggcgc
     2521 cgaaggccga caggccggtg tcacacgagg agcaggctgc cgcgacgacg agaaggatgt
     2581 aggcgagtcg ca
//
LOCUS       DAOSPL010000598         2591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39769_length_2591_cov_3.964905, whole genome shotgun sequence.
ACCESSION   DAOSPL010000598 DAOSPL010000000
VERSION     DAOSPL010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39769_length_2591_cov_3.964905"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PLQ54_11210"
     CDS             complement(<1..716)
                     /locus_tag="PLQ54_11210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS93873.1"
                     /translation="MSQPFPARSEGAPSLRVDGLTLGYGRRPAVLEDVSFEARHGVTL
                     LLGANGAGKSTLIATLARAHRPRRGRWHLNADGVEFGDRDTRLRTGLLPQSPAFSSRM
                     TGLQCVEYVAWLRRVPRARRATNVSMALEVANATHVLDVPWSRMSYGTKQRVAIAAAL
                     VNKPSLLLLDEPLNGLDIGEKHSLIGVLGALPWPAVSLVSSHVVEGLEEIATNVLVLT
                     GGRVAYAGPSAELVDAHGVVG"
     gene            complement(768..1181)
                     /locus_tag="PLQ54_11215"
     CDS             complement(768..1181)
                     /locus_tag="PLQ54_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93874.1"
                     /translation="MKTRRSKIGTAVVIALLAAQPGVAWAVSDVSGSYLSYKTDLKKR
                     DTGTYAGPTGGQIRLCADQQGTVTGQGGSIAYIKRDVSALPDTTNSSMVVEGGNAAKC
                     TSYTSSSSNARYYTRVQPSWDGGGNYTGWAKAQKP"
     gene            complement(1322..1786)
                     /locus_tag="PLQ54_11220"
     CDS             complement(1322..1786)
                     /locus_tag="PLQ54_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93875.1"
                     /translation="MASASLVGMDKRKKDLEVIPEAESAYGDWKGTSCAENSLMVTSG
                     DLYELAGLAAERDRWLIVGIDIHAHSHGADPRWTIHVYAADLEELGATGVNGLNQVAA
                     KHGGIPVVDVLLHDATLDDVIKCMKLLNVQLRNSYLQHPLLHAAYADHPAQD"
     gene            complement(1827..>2591)
                     /locus_tag="PLQ54_11225"
     CDS             complement(1827..>2591)
                     /locus_tag="PLQ54_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93876.1"
                     /translation="LPVQVHKVGVPTATSAARQWVGLRQIVLASSSLPNSPSGRLNHL
                     QATPARLDGELPEVLAAARVLTHRALVTNNPGHEDITSAMVVLDAITTLLRPEQATLT
                     ATFWSSLPRSTRTLTPSSSGYRQAMEHVTARLQRADQPMPPLAHTAAVATLTATMDDL
                     RAAWSSIAAKGDLVVSTRHVPSSQTGLDARAYLDARRRHHWTPLPPEVPKRSARVLAL
                     HQAQVATITAVPAAATPAARVVEYSAGITPTGITPT"
BASE COUNT          402 a          838 c          898 g          453 t
ORIGIN      
        1 ccgacaacac cgtgcgcgtc gaccagctcg gcggatggcc ccgcgtaggc cacccgcccg
       61 ccggtcaaga ccagcacatt cgtcgcgatc tcctccaagc cttcaaccac gtggctggac
      121 accaacgaaa ccgccggcca cggcagcgca cccagaacac ctatgagcga gtgcttctcc
      181 ccaatgtcca ggccgttcaa gggttcatcg aggagcaata gcgaaggctt gttcaccaac
      241 gcggcggcaa tcgcgacacg ttgtttggtg ccgtaggaca tccgcgacca cggcacgtcg
      301 agcacgtggg tggcattggc tacctcaagg gccatgctca cgttggtggc cctcctcgcc
      361 cgcgggacgc gccggagcca cgccacgtac tcgacacact gcaagcccgt catacggctg
      421 gagaacgctg gagactgcgg caacagaccg gtccgcaggc gggtgtcgcg atcaccgaac
      481 tcgacaccgt ccgcgttcaa gtgccagcga cctcgccggg gccgatgggc ccgagccaac
      541 gtcgcgatca gggtggactt gcctgccccg ttggctccca gaagaagcgt gaccccatgg
      601 cgggcttcga acgacacatc ctcaaggacc gcgggccgcc gcccgtaccc gagcgtcagg
      661 ccatcgaccc gcagtgaggg cgcaccctca ctgcgggctg ggaacggctg agacacgcca
      721 cccgggcgca actcatccag agcgctgggc cctcgtaggc cgcggggtca cggcttctgg
      781 gcctttgccc agccggtgta gttaccgccg ccgtcccacg acggctgcac cctcgtgtag
      841 taccgagcat tcgagctcga cgatgtgtaa ctggtgcact tggcggcgtt cccgccctcg
      901 acaaccatgc tgctgttggt ggtgtcgggc agtgcagaga catcgcgctt gatgtaggcg
      961 atagatccgc cctggcccgt cacggtgcct tgctggtcgg cacacagtcg gatctgcccg
     1021 ccggtcgggc ccgcataggt tccggtgtca cgcttcttca ggtcagtctt gtatgacaag
     1081 tagctgcccg acacgtctga cacagcccaa gcgactcccg gctgcgcagc cagaagtgca
     1141 atcacgactg ctgtaccgat cttggatcga cgagtcttca taagcccctc cttggatttt
     1201 ccccggaatc gggcactggc ccgattacgg ctgacgctag cagtagtttg ctcgcgcccg
     1261 aggcccaaac cgaagccgtc ttcgatggtc tcctgaggcg gcctgtcccg gtgccctgga
     1321 ctcagtcctg agcggggtgg tcggcgtagg cggcgtgcag tagggggtgc tggaggtagc
     1381 tgttgcggag ctggacgttg aggagcttca tgcacttgat cacgtcgtcg agggtggcgt
     1441 cgtgcagcag gacgtcgacg acggggatgc cgccgtgctt tgcggcgacc tggttgagtc
     1501 cgttgacgcc ggtggctccg agttcctcga ggtcggctgc gtagacgtgg atcgtccacc
     1561 gggggtccgc gccgtggctg tgggcatgga tgtcgatgcc gacgatgagc caccggtccc
     1621 gctccgctgc gaggcccgcc agctcgtaga ggtcgccgct ggtgaccatg aggctgttct
     1681 cggcgcacga ggtgcctttc cagtcgccat aggccgactc tgcctcgggt atgacctcga
     1741 ggtccttctt gcgcttgtcc atacccacca ggctggcaga ggccaccgac aactcgccga
     1801 cgtgcagttc cccgcacccg agggggctac gtcggcgtga tcccagtggg tgtgattccg
     1861 gcggaatact cgaccactct tgctgcgggc gtcgccgctg ccgggacggc tgtgatggtc
     1921 gcgacctggg cttggtggag agcgagaacg cgtgcgctgc gcttgggcac ctcgggcggg
     1981 agcggtgtcc agtggtgacg tcgacgggcg tcgaggtacg cgcgagcgtc caggccggtc
     2041 tgggatgagg ggacgtgcct ggtgctgacg accaggtcgc ccttggcggc gatgctcgac
     2101 catgcggcgc gcaggtcgtc catcgttgcc gtgagtgtcg ccaccgcggc ggtgtgcgcc
     2161 agcgggggca tcggctggtc cgcgcgctga agccgtgcgg tgacgtgttc catcgcctgg
     2221 cggtagccgg acgaggaggg cgtcagcgtg cgcgtggatc gcggtagcga tgaccagaac
     2281 gtcgccgtga gggtcgcctg ctcgggtcgc aggagagttg tgatcgcgtc gaggacgacc
     2341 atcgctgagg tgatgtcctc gtggcctggg ttgttggtga ccagcgcccg gtgggtgagg
     2401 actcgggctg ccgccaggac ctcgggcagt tccccgtcga gccgggcagg ggtggcctgg
     2461 aggtggttca gacgccctga cggggagttg ggcagcgagc tcgaggcgag aacgatctgc
     2521 cgcagcccca cccactgccg cgccgcggac gtcgccgtgg gcacgcccac cttgtgtacc
     2581 tgcacgggca g
//
LOCUS       DAOSPL010000599         2583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39935_length_2583_cov_3.460839, whole genome shotgun sequence.
ACCESSION   DAOSPL010000599 DAOSPL010000000
VERSION     DAOSPL010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39935_length_2583_cov_3.460839"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLQ54_11230"
     CDS             <1..202
                     /locus_tag="PLQ54_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93877.1"
                     /translation="MVLDGKMVVRTVGDSLDDLLRLAPKSEHVFTMENGGSKGEDAVF
                     TVDEAGRVTKLRLGGYAYLSDH"
     gene            complement(156..1157)
                     /locus_tag="PLQ54_11235"
     CDS             complement(156..1157)
                     /locus_tag="PLQ54_11235"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOS93878.1"
                     /translation="MSDLRYVMGIDGGGSKTDAVLMDETGAVVGWGRGGPTHAGFEPD
                     EAVRAAVPSAVAAALNGRLIRVSAIVTSFCGPQAIAAAHELSAGADVHPVGETDVVFA
                     ANQVDAGLIVLASTGSGVRARNRQGESVHMGGFGPVIGDEGSAYAIGVRAIRACIYAH
                     WSSEWDTSLRERVLEATHCARVYDLIGYFHYGGSTRREVAALAPLVDQESAAGDPVAG
                     RILAESARELGVLALCALRRANMLDASYPMLPSGSVARNSAAYWKAMTEFVCASAPLI
                     EPQAPGPAPAVGGALLALRRLGVEWTGDVVTRARTTALEWSQPSGHSGTHTPQAGVW"
     gene            1288..2292
                     /locus_tag="PLQ54_11240"
     CDS             1288..2292
                     /locus_tag="PLQ54_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS93879.1"
                     /translation="MEYVRFGNSGLEVSRLCLGAMDFPLRCDRETSRSIFFRAFEAGI
                     NFIDTADAYGPGTSEEVMGDLLHGVRDQLVIATKFWVKMYDRPGGRGCSRYHIMSAVE
                     DSLRRLRTDHIDLYQLHHPDHMSPVEETLSTLDALVTQGKVRYIGVCNHYAWQMAHML
                     GVSALHNWEPLISIQCRYNIVDRVIETETVPFAKRFGIATMVYGPLHGGILAGAYRRG
                     QPLPEGSRYERIQGYGQKHLTPQVYDLLDELEPIAQRNGLTLGQLAMKWILSKDFVTT
                     PILGGSRPEHFEPMYDVLSKQVDADDLRRIDELSEPYRYKAFENQPVAGGAPLAHNWW
                     "
BASE COUNT          475 a          812 c          863 g          433 t
ORIGIN      
        1 catggttctg gatgggaaga tggtggtccg gacggtcggg gactctctcg acgacctgct
       61 acgcctggcg ccaaagtcgg aacacgtgtt caccatggag aacggcggca gcaaggggga
      121 ggacgcggtg ttcactgtcg atgaggccgg ccgagtcacc aaactccggc ttggggggta
      181 tgcgtacctg agtgaccact aggctgagac cattcgagcg ccgtggtccg ggcgcgagtc
      241 acaacatcgc cagtccactc aacgcccagc cggcggagag cgagaagggc accgccgact
      301 gcgggggcgg ggccgggcgc ctgcggctcg atcagaggcg ccgatgcaca cacaaactcc
      361 gtcatggcct tccagtaggc cgcgctgttt cgtgcgaccg agcccgaggg caacatgggg
      421 taggatgcat cgagcatgtt ggcgcggcgc agcgcgcaca gcgccaacac gccgagttcg
      481 cgggcgctct cggcgaggat gcggccagcg acgggatcgc cggccgcgga ttcctggtcg
      541 accaggggag ccagggcggc cacctctcga cgcgtcgatc cgccatagtg gaagtaaccg
      601 atcaggtcgt agacgcgcgc gcagtgagtt gcctcgagca cgcgctcgcg cagcgaagtg
      661 tcccactcgg atgaccagtg ggcgtagatg cacgcacgga tggcccggac gccgatggca
      721 taggcgctgc cctcatcgcc gatgacggga ccgaatccgc ccatatggac cgactcgcct
      781 tggcgattcc gggcgcggac cccactgccc gtgctggcca acacgataag ccccgcatcc
      841 acctggttgg cggcgaagac tacgtcggtc tcccctacgg ggtgcacgtc ggcgccggcg
      901 ctgagttcgt gggcggccgc aatggcctgg gggccacaga agctggtcac gatggctgag
      961 acgcggatca gccggccgtt cagtgcggca gcgacggccg atgggacggc ggctcgcacg
     1021 gcttcatcgg gttcgaagcc cgcatgggtc gggccaccgc gcccccagcc caccacggcg
     1081 ccggtctcat ccatcaggac ggcatccgtc ttgcttccgc cgccatcgat ccccatgaca
     1141 tagcgcaggt cagacactca gagacctctc tcccgagacc ggcatggttc cgtccccgtc
     1201 agctggattg taggacatgg cgggcgagtg cgggaatccc ccgaaccggc cgtgcgggta
     1261 tccagcatgg ttcgaggagg gtgctcgatg gagtatgtgc gctttggcaa ctcgggcctt
     1321 gaggtgtctc gcctgtgcct gggtgccatg gacttccccc tgcgttgcga ccgcgagacg
     1381 tcacggtcca tcttcttccg ggcatttgag gcgggcatta acttcatcga cacggccgac
     1441 gcctacggcc cgggcaccag cgaggaggtg atgggcgatc tgctgcacgg ggtgcgtgac
     1501 cagctcgtca tcgccacgaa gttctgggtg aagatgtacg accgccctgg cggacgcggc
     1561 tgttcgcgct atcacatcat gagtgccgtc gaagacagcc tgcgccggct gcgcaccgat
     1621 cacatcgatc tataccagct tcaccacccg gaccacatgt cgccggtgga ggagacgctg
     1681 tcgacgctcg acgcactagt gacgcaaggt aaggttcgat atataggtgt ctgcaaccac
     1741 tacgcgtggc agatggccca tatgctcgga gtgtcggcgc tccacaactg ggaaccgctg
     1801 atatcgatcc agtgccggta caacattgtc gatcgcgtga tcgagacgga gactgtgccg
     1861 ttcgcgaagc ggtttggaat cgccaccatg gtctacgggc cgctccacgg cgggatcctg
     1921 gcgggcgcct accggcgggg acagcccctg ccggagggga gtcggtacga gcggatccag
     1981 ggctacggcc agaaacatct aacgccgcag gtctatgacc tcctcgatga gctggagccc
     2041 atagcccagc gcaatgggct gacgctgggg cagctggcga tgaagtggat tctgagcaag
     2101 gatttcgtga ccacacccat cctgggtggc agccgacccg agcatttcga gcccatgtac
     2161 gatgtgctga gcaaacaggt ggacgcggac gacctccgcc gcatcgatga gctctcggag
     2221 ccgtatcggt ataaggcatt cgagaaccag cccgtagccg gcggcgcccc gcttgctcac
     2281 aactggtggt agaaacagac aagcgattcc gggtcactcc gagccgttgg cgtggaaccc
     2341 tctggctgtg cacccggtgt cggaaccaag ctccaggacc ccacaccgga actgcgaggt
     2401 gaccgaaaca tgcgcgccat gttgctcgcg atggctttcg gcctcggagc agggcttgcg
     2461 ggctgcacgg ttcaggtgga cttgccgtcc gtgaacctgg gcacaggcag ttcatcgctc
     2521 cggaccggtt tccagagggt gatcatcgat ctctcgacca acgagcagtt catgcagtat
     2581 cag
//
LOCUS       DAOSPL010000600         2583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39940_length_2583_cov_3.242880, whole genome shotgun sequence.
ACCESSION   DAOSPL010000600 DAOSPL010000000
VERSION     DAOSPL010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39940_length_2583_cov_3.242880"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLQ54_11245"
     CDS             complement(<1..510)
                     /locus_tag="PLQ54_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93880.1"
                     /translation="MLRKSAGFALVLVLAATVPCWAQFAGHTGTARQMGMGGAQVAIA
                     DDASSAASNPAGLARLRAYWTGATAQDEDVFLFGDDNGRSDADQNEVLATGGVQALPA
                     LGVARSTRAGVAFAGGPDDLDSGYGLTADIAGIGVTPSLIWSLGYGAQFGGTSKLSWG
                     AGFAGVTQQA"
     gene            783..2192
                     /locus_tag="PLQ54_11250"
     CDS             783..2192
                     /locus_tag="PLQ54_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOS93881.1"
                     /translation="MSHATKATLLPELRYCWVPPEPGADCWMVCLSLTAIAPGGEPIE
                     RRPLSVCFVIDSSGSMYFSLMSKRELSRWAQIARSRGELATVVTDGRNMTTVVGETRR
                     EMMEADVTPMHFVVRAIEGALGKLDPDDEAGVIAFADRGRLIAQGAVGSLDLSALNDL
                     SHRRYVRDLGTGTQLATGLGPCLDWLGERRYSDTRKKVILFSDGLVDDRPRAALSVKR
                     LMQTGASISTFGLGVEFDEDMLAQIADDAGGDYHFMETPDQVAQALEEEIMAERSLVT
                     GDIHLGLRPEMGLTLGDLYQVLPFVKVNEPLRRTMVDRVYSAGAMGAGEERVFLLELR
                     GAPSSPGGPAIEVELYPAPSGPNEPPKGVVSRVLDPPGGAGTATDLEPRATHLARRVK
                     AFAIQRQARQAAEAGDERGAAAKMEALSQVLNSLGESDLAGVAQEEAASLRGETAADS
                     GRTKRLKAATRKITRGRGL"
     gene            2235..>2583
                     /locus_tag="PLQ54_11255"
     CDS             2235..>2583
                     /locus_tag="PLQ54_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOS93882.1"
                     /translation="MADESGSAPNPAARAIATLLVNCNGHEREVAVEAECITLGRDDP
                     YEEGAQHVQLIEDDTVSRRHARLVVRADWACAVDLDSTNGTRVNGIPLTPAIEVVIHD
                     GDEIALGEHTHIVC"
BASE COUNT          466 a          936 c          810 g          371 t
ORIGIN      
        1 cgcctgctgc gtgacgccgg cgaaccccgc gccccaactc agcttcgagg tcccgccgaa
       61 ctgcgcgcca tagccgaggc tccaaatcag ggacggcgtg acaccgatcc ccgcgatgtc
      121 ggcagtcaac ccgtaaccgg agtccaggtc gtccggtccg cccgcgaagg ccaccccggc
      181 gcgggtcgag cgagccacgc cgagagccgg cagagcctgc acgccgcccg tggccagcac
      241 ctcgttctga tccgcgtcac tgcggccatt atcgtcgccg aacaagaaga cgtcctcatc
      301 ctgggcggtg gcaccagtcc agtacgcccg aagccgcgcc agccccgccg gattggacgc
      361 ggccgaggag gcatcgtccg cgatcgcgac ctgagcacca cccatgccca tctggcgcgc
      421 ggtcccggtg tgaccggcaa actgcgccca gcacggcacc gtcgcggcga gcaccagcac
      481 caaagcaaac ccggctgact tcctcagcat caaaggtcct cccagctcag tcgagcgtga
      541 ccgccctcat acctcgtgaa ccccgcacca tgcggtccgc acagaaaaac ccaccacgcc
      601 gccacccgcg gcggggccgg tccatccacc accgatctaa cgctctaacg acgcagggtt
      661 cgcaaatgtg caatccctct cctcctcttc gccatgcttt ttcgccggcg gctccaggcg
      721 cctccgagga ctccgaacgg cctcaagcga agaaacgaat gccccaaggg agctggacgc
      781 tgatgtctca cgccaccaaa gcaaccttgc tgcccgagct ccggtactgc tgggtgcccc
      841 ccgagcccgg cgccgattgc tggatggtct gcctgtctct cacggccatc gcgccgggcg
      901 gcgagcccat cgagaggcgg cccttgtcgg tctgtttcgt catcgacagc agtggatcca
      961 tgtacttctc gctaatgtcc aagcgtgagc tgtcgcgctg ggcacagatc gcgcggtccc
     1021 ggggcgagtt ggccaccgtc gtcaccgatg gccggaacat gaccactgtg gtgggcgaga
     1081 cccgtcgcga gatgatggaa gccgacgtca cgcccatgca cttcgtggtt cgagccatcg
     1141 agggcgccct cggcaagttg gaccccgacg acgaggccgg agtcatcgcc ttcgccgaca
     1201 ggggccgact catcgcccaa ggcgcggtgg gctccctgga cctcagcgcg ctcaacgacc
     1261 tctcccatcg ccgctacgtc cgcgacctcg gaacgggcac gcagctcgcc accggattgg
     1321 ggccctgcct cgattggctg ggcgaacggc gctacagtga cacccgcaag aaggtcatcc
     1381 tcttctccga cggcctcgtc gacgatcggc cccgggcggc cctatcggtc aaacggctca
     1441 tgcagaccgg agcgtccatc agcacctttg gcctcggcgt cgagttcgat gaggacatgc
     1501 tcgctcagat cgccgacgat gccggcggcg actaccactt catggagaca ccggaccagg
     1561 tcgcccaggc cctcgaagag gagatcatgg ccgaacgctc cctggtcacc ggcgatatcc
     1621 acctgggcct gcggcctgaa atgggcctca ccctgggaga cctgtaccag gttctgccgt
     1681 tcgttaaggt caacgaaccc ctccggcgaa ccatggtcga ccgggtctac agcgccgggg
     1741 ccatgggggc cggcgaggaa cgcgtgttct tgcttgagct tcgtggcgcg ccgagcagcc
     1801 cgggcggccc agccatcgag gtcgagctct acccggcccc gtcggggccc aacgaaccgc
     1861 ccaaaggcgt ggtcagccgc gtgctggatc cgcccggcgg cgccggaacg gccaccgacc
     1921 tcgagccccg cgcaactcac ctggcccggc gcgtcaaggc attcgccatt cagaggcaag
     1981 cacgtcaggc cgcggaggcc ggcgacgaac gcggagcggc cgccaagatg gaggcgctct
     2041 cccaggtcct caactcgctc ggcgagtcgg atctggccgg cgttgcacag gaagaggcgg
     2101 ccagcctgcg cggcgagacg gccgccgata gcggccgcac caagcgactc aaagcggcaa
     2161 ctcgaaagat cacccgaggc cgcgggctct aggcagcccg tcggaccctg gcgcccggag
     2221 aggacgagtg accattggcg gacgagagcg ggtcggcccc caaccccgcg gcacgagcca
     2281 tcgcgaccct tttggtgaac tgcaacggac atgagcgaga ggttgcggtc gaggccgaat
     2341 gcatcacgct cgggcgggat gacccctatg aagagggcgc ccagcatgtg cagctcatcg
     2401 aggacgatac cgtctcgcgt cggcacgctc gcctggtagt ccgtgccgac tgggcctgcg
     2461 ccgtagacct cgacagcacc aacggcacgc gggtcaacgg cataccactc actccggcca
     2521 tcgaggtcgt cattcacgat ggcgatgaga ttgcgctggg tgagcacaca cacatcgtgt
     2581 gcc
//
LOCUS       DAOSPL010000601         2582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39963_length_2582_cov_3.020973, whole genome shotgun sequence.
ACCESSION   DAOSPL010000601 DAOSPL010000000
VERSION     DAOSPL010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39963_length_2582_cov_3.020973"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PLQ54_11260"
     CDS             <1..559
                     /locus_tag="PLQ54_11260"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOS93883.1"
                     /translation="ELAKLDLRFCIGCGRCYAAGSCIHDDGVAAVQRRMVDADGVVLA
                     SPNYMHSVTAQMKAFMDRCSLHVHCFLLTGKYGAGVATAGGGGEERVAGFENEFLRLC
                     GAQTVGIAAALAAGPAALRDQEAALAGGRDLGADLVAAIRERRQYPDQAEEHAAFAGR
                     MKQLVARMAEHAPFQYEYWEKMGWL"
     gene            588..1985
                     /locus_tag="PLQ54_11265"
     CDS             588..1985
                     /locus_tag="PLQ54_11265"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HOS93884.1"
                     /translation="MPKPRIAIAGIGHESNTYSTRMTGRADFRILRGPEIGDDASLPA
                     AAEVDWAPALVAYAAPNGIVARDAYEALKAELLERLSAATPLDGVLLRLHGAMEVEGI
                     GDGETDLLMGVRDAVGPDALVSGRLDLHANLSPLAAATADVLTAHRTAPHVDARETLG
                     RAARHLIRALADRLRPRNCLVKLPLRLPGEFAVTDHEPARSLYARLVELECHPGILDA
                     SLAIGCAWTDSAHAGVGTWLVAESEPHQARALALAQEFAETIWRRRGEFGPDVPTGEL
                     PVVLERALASAQRPVVIADSGDNPGAGGAGDTTVVLEALLAAGVTGALVAGIADAEAV
                     GACCRAGEGESATLALGGKLDRTSGRPLELRVEVERVVPGVLAVVRAEGVRLIITSSR
                     SQLSEESHYLEAGLDPSAYRIVVVKCGYMSSYLKGIAGERLMALTPGFTTLRPACVVD
                     RATGWATPAPGGWGG"
     gene            complement(2130..>2582)
                     /gene="araH"
                     /locus_tag="PLQ54_11270"
     CDS             complement(2130..>2582)
                     /gene="araH"
                     /locus_tag="PLQ54_11270"
                     /EC_number="7.5.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690124.1"
                     /note="Part of the ABC transporter complex AraFGH involved
                     in the high affinity transport of arabinose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HOS93885.1"
                     /translation="LLVRTTLGPNTLAIGGNAEAARLAGIPVDRLKILIFTMQGLMAG
                     LAGVVLAARMTSGQPNCSVGLSLDVISACVLGGVSLSGGVGTIHGVIIGVLVMGAVQN
                     AMNLRNVETFYQYVARGLILLAAVLFDQIRRGQGAGLWRQVRRWFPVG"
BASE COUNT          390 a          858 c          960 g          374 t
ORIGIN      
        1 cgagctggcg aagctcgatc tcaggttctg catcgggtgc ggccggtgct acgccgccgg
       61 cagctgcatc cacgacgacg gcgtggctgc cgtgcagagg cggatggtcg atgccgatgg
      121 tgtggtgctg gccagcccca actacatgca ctcggtgacc gcgcagatga aggccttcat
      181 ggaccgctgc agtctccacg tgcactgttt cttgctgacc gggaagtacg gcgccggcgt
      241 ggccacggcg ggtggcgggg gcgaagagcg cgtcgcgggt tttgagaacg agttcctgcg
      301 gctctgcggc gctcagaccg tgggcatcgc cgccgcactg gcggccggac cggcggcgct
      361 gcgagaccag gaggccgcgc tcgccggggg gcgggacctg ggcgccgact tggtggccgc
      421 gatccgcgag cgcaggcagt accccgatca ggcggaagag cacgcggcct tcgcggggcg
      481 gatgaagcag cttgttgccc gcatggccga acacgctccg ttccagtatg agtactggga
      541 gaagatgggg tggctgtagg cggttcgggc ccaaggggtg gcttgccgtg ccgaaaccga
      601 ggattgccat cgccggcatc gggcacgaga gcaacacgta ctcgacacgc atgacggggc
      661 gagcggattt ccgcatcctg cgcgggccgg agattgggga cgacgcgtca ctgcctgcgg
      721 ccgcggaggt ggactgggcg ccagccctgg tggcctacgc ggcaccgaac gggatcgtcg
      781 cgcgcgatgc ctacgaggcg ctcaaggccg agctcctcga gcgactcagc gccgccacgc
      841 ccctcgatgg cgtcctgctg cgcctgcacg gggccatgga ggttgagggg atcggtgacg
      901 gcgagacgga cctcttgatg ggcgttcgcg acgcggttgg gccggacgcg ctcgtgtccg
      961 ggcggcttga cctgcatgcg aacctgtcgc ccctcgccgc cgcgaccgcc gacgtgctga
     1021 ccgcgcaccg caccgcaccg catgtggacg cgcgggagac gctgggccgg gcggctcgcc
     1081 acctgatccg agcgctggcc gaccggctgc gcccacgcaa ctgcctggtg aagctgcccc
     1141 tgcgccttcc gggcgagttc gcggtgacgg atcacgagcc ggcccggtcg ctctacgccc
     1201 ggttggtgga gctggagtgc cacccgggca tcctcgatgc gtccctggcc atcgggtgcg
     1261 cctggaccga ttccgcgcat gcgggtgtcg gcacgtggtt ggtggcggag tcggaacctc
     1321 accaggcccg cgcgctggca ctggcgcagg agttcgccga gaccatctgg cgccggcgcg
     1381 gggagttcgg gcccgacgtg cccacgggcg agctgcccgt cgtgctcgag cgcgcgctgg
     1441 ccagcgctca gcgccccgtg gtcatcgctg actccggcga caaccccggc gccggcgggg
     1501 cgggggacac gacggtcgtg ctcgaggcgc tgctagcggc gggcgtcacc ggggcactgg
     1561 tggcaggcat cgcggatgca gaggccgtcg gggcctgctg ccgagccggc gaaggcgagt
     1621 ccgccacact ggcactgggc gggaagctgg accggaccag cggccggcca ctcgaactgc
     1681 gcgtcgaggt tgagcgcgtg gtccctgggg ttctggccgt ggtccgcgcc gagggtgtac
     1741 gcctcatcat cacgtcatcg aggagccagc tctcggagga gagccactac ctggaggccg
     1801 gcctggatcc ctcggcctac cggatagtcg tggtcaagtg cgggtacatg tccagctacc
     1861 tgaaaggcat cgcgggtgag cggctaatgg cgctaacgcc gggcttcacc acactccgcc
     1921 cggcgtgtgt cgtcgatcgg gcaaccggat gggccacgcc ggccccgggg gggtggggcg
     1981 ggtagacgag gcgatgccga gaacgcctcg cgagagccgt ggggactccc gctcgcgccg
     2041 cccttggtcg cgccgcgcga acgcgacggc tcaggaacga cggccttggg ggcctgagat
     2101 gtgggcgcgg ggcctgcctc gccctggctt cacccgaccg gaaaccacct gcgtacctgc
     2161 cgccagagtc cggcgccctg gccgcgccgg atctggtcga acagtactgc ggcgaggagg
     2221 atgaggccgc gagcgacata ctggtagaag gtctccacat tccggagatt catggcgttc
     2281 tggaccgccc ccatgaccaa gacgccgatg atcaccccat gaatggtccc cacgccgccc
     2341 gagagcgaga cccctccgag aacacatgcc gagatgacgt cgagcgacaa gcccaccgag
     2401 cagttgggct gaccactcgt catgcgcgcc gccagcacca cgccagcgag gccggccata
     2461 agcccttgca tggtgaagat caggatcttg aggcggtcca ctgggatacc cgccaaccgg
     2521 gctgcttcgg cgttgccgcc aatggcgagg gtgttcggac caagagtcgt ccggaccagc
     2581 ag
//
LOCUS       DAOSPL010000602         2582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39968_length_2582_cov_2.551642, whole genome shotgun sequence.
ACCESSION   DAOSPL010000602 DAOSPL010000000
VERSION     DAOSPL010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39968_length_2582_cov_2.551642"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1811
                     /gene="glgP"
                     /locus_tag="PLQ54_11275"
     CDS             <1..1811
                     /gene="glgP"
                     /locus_tag="PLQ54_11275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOS93886.1"
                     /translation="QEVVAGIGGVRALEALGIRPTVCHMNEGHCAFLALERIRRVIET
                     EGLTFGEAAEALAVSHVFTTHTPVPAGHDVFDQTLMDRYFSHYWPQLGLTREQFLGLG
                     REDPTDANSPFNMTVLAVRLSTWRNGVSELHGAVARRQLRALWPNVPDDDIPIDSVTN
                     GVHPTSWISYDMSGLFDRYLGPWWREQSGEEKIWQGIYEIPDTELWRTHERRRERLVA
                     YARRRLRQQLIARGASPAEVAQADEVLDPEALTIGFGRRFATYKRATLLLRNPDRLIE
                     LLANKDQPLQIVLAGKAHPNDRPGKDLIRQIVHFARRPECRGRIVFLEDYDMSLARYM
                     LQGVDVWLNTPRRPNEASGTSGMKATMNGALNVSILDGWWCEGYSPDTGWAIGRGEEY
                     QDFDYQDDVEAQGLYDLLEKEVIPLFYHRGADGLPRGWIARMKHAIRRLTPQFSSNRM
                     VQEYAEKFYLPAAQRAEAMKADNYARARALGQWKQKVRQAWSQIRIVAVDDDHGMGTE
                     VGQKITVTAQVDLGPLTPEDVHVELFHGRVDSKGEIVEAINTRMQCIQDEPSQPRVFR
                     AEIQCRQAGQRGYTVRVIPEHEDLVCPFDMGLILWA"
     gene            1887..2228
                     /locus_tag="PLQ54_11280"
     CDS             1887..2228
                     /locus_tag="PLQ54_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOS93887.1"
                     /translation="MDRTGAPLAVNVVRADRWLSRVVGLMFRSRLEPGEALWIPRCSA
                     VHTCFMRFRIDLVFLDESLRVTRIAESVGPFRARYGGRQARSVLELAAGTASRLGLRA
                     GDRVSVTRSDR"
     gene            2242..>2582
                     /locus_tag="PLQ54_11285"
     CDS             2242..>2582
                     /locus_tag="PLQ54_11285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS93888.1"
                     /translation="MRVAMFSECYEPTSNGVVVSMQSFLRELEARGHEVFVFAPAYRG
                     HRDTAPRVYRFPSIPTPFNRDYPLALPFPRLPRGLERWGLDVVHTQHPFTMGKAGREA
                     GWALLMPVIILG"
BASE COUNT          436 a          799 c          891 g          456 t
ORIGIN      
        1 gccaagaggt cgtggcaggc atcggggggg tccgcgcact cgaggccctg gggattcggc
       61 ccacggtctg ccacatgaac gaggggcact gcgccttcct ggcgctggag cgcattcggc
      121 gagtcatcga gaccgagggg ctcacctttg gcgaggcggc cgaggcgttg gccgtgagcc
      181 atgtgttcac gacccacacg ccggtacctg cgggccatga tgtgttcgac cagacgctta
      241 tggaccgcta cttcagtcac tactggccgc agctcgggct gacgagagag cagttcctgg
      301 gcttggggcg cgaggacccc accgacgcga actcgccctt caacatgacg gtgttggccg
      361 tgcgcctctc gacatggcgc aatggtgttt cggaactcca tggggctgtg gcccgccgtc
      421 agctccgggc tctgtggccg aacgtgcctg acgatgatat ccccatcgac tcggtgacca
      481 atggcgtgca tcctacctcg tggatctcct acgacatgtc tgggctcttc gatcgctatc
      541 tggggccgtg gtggcgagag cagtcgggcg aggagaagat ctggcaggga atctacgaga
      601 tcccggatac cgagctctgg cggactcatg agagacggcg cgagcggctg gtcgcctacg
      661 cccggcgccg gctccggcag caactgatcg cccgcggtgc gagcccggcg gaagtggccc
      721 aagccgacga ggtcctcgat ccggaggcgc tgaccatcgg ctttggccgg cgcttcgcca
      781 cctacaagcg ggccaccctc ctgttgagga accccgatcg gctcatcgag ctgctggcga
      841 acaaggacca gcctctgcag atcgtcctcg cgggtaaagc ccacccgaac gaccgtccgg
      901 gcaaggacct gattcgccag atcgtgcact tcgcgcggcg gcccgagtgc cggggccgca
      961 tcgtgttcct cgaagactat gacatgagtc tggcgcggta catgctccag ggtgtcgatg
     1021 tgtggctcaa caccccgcgg cggccgaatg aggccagtgg cacgagtggc atgaaggcga
     1081 cgatgaacgg ggccctcaat gtgagcattc tcgacggttg gtggtgcgag ggttactcgc
     1141 ccgacaccgg gtgggccatc ggccggggag aggagtacca ggacttcgac tatcaggatg
     1201 atgtcgaggc ccaagggctg tacgacctgc tcgagaaaga ggtcatcccg ctcttctacc
     1261 atcgtggggc cgacggactc ccccggggct ggatcgcgcg gatgaagcac gccatccgtc
     1321 gcttgacgcc gcagttcagc tcgaaccgca tggtccagga gtatgctgag aagttctacc
     1381 tcccggctgc ccaacgggcc gaggccatga aagcggacaa ctatgcccgg gcacgggcgc
     1441 tggggcagtg gaagcagaag gtacggcaag cctggagtca gatccgcatc gtggccgtcg
     1501 atgacgacca cggcatgggt acagaagtgg gccagaagat cacggtgacc gctcaggttg
     1561 acctggggcc cctcacgccg gaggatgtcc atgtggagct gttccacggc cgagtggatt
     1621 ccaagggcga gatcgtggag gccattaaca cccgcatgca gtgcattcag gatgagccct
     1681 cacagccccg ggtgttccgg gccgagatcc agtgtcggca ggccggtcag cgcgggtaca
     1741 cggttcgagt gattcccgag cacgaggatc tggtgtgtcc cttcgacatg ggcttgatcc
     1801 tctgggcgta ggccggcatg tcgcgaaaga gaccacggca gcgccgacgg cgtccccgca
     1861 gcggggacgc cgctcgtgcc tgtgtcatgg accgaacggg agcgcccctg gccgtgaacg
     1921 tggtccgggc cgatcgctgg ctctcacgcg tcgtgggact catgtttcgg tcccggctgg
     1981 aaccagggga ggcgctgtgg ataccgcggt gttcggcggt tcacacgtgc ttcatgaggt
     2041 tccggatcga cctggtgttc ctggacgagt cgctgcgggt cactcgcatc gccgaatcgg
     2101 tcggcccgtt ccgagcccgc tatggtggca ggcaagccag gtcagtcctc gagctcgccg
     2161 cggggacagc gtcccggctc ggactccgtg cgggcgaccg cgtgagcgtg acgcggagcg
     2221 atcgctgaag ggtctctgca gttgagagtc gcgatgttct ccgaatgcta tgagcccaca
     2281 tcgaacggcg tggttgtgtc gatgcagagc ttcctgcgtg agctcgaagc gcgcggtcac
     2341 gaggtctttg tgtttgcccc tgcgtaccgg ggccaccggg atacggcacc gcgcgtgtac
     2401 cggttcccgt cgataccgac gcccttcaac cgcgactatc cactggcgct gcctttcccg
     2461 cgcctgcccc gcggactgga gaggtggggt ctcgatgtcg tacacaccca gcatccgttc
     2521 accatgggca aggcggggcg cgaagccggc tgggccttgc tgatgccggt gatcattctg
     2581 gg
//
LOCUS       DAOSPL010000603         2581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_39985_length_2581_cov_3.639351, whole genome shotgun sequence.
ACCESSION   DAOSPL010000603 DAOSPL010000000
VERSION     DAOSPL010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39985_length_2581_cov_3.639351"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PLQ54_11290"
     CDS             complement(<1..1080)
                     /locus_tag="PLQ54_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HOS93889.1"
                     /translation="MGQATQELQKQAQAARPHVTRKPEKTQVLVGIATCALAAGAEKT
                     WQQLETAIAEAGLGDRVDLHQVGCVGRCNLEPLVEVRVPGQPARMYTKVADADKVRTI
                     VNRDLLGGDPVTEWMLPVPDGAPEADPSSVSAFIYEWRHVDFFARQLRITLRNVGRVD
                     PESIDDYLAAGGYQALAKVLDEMTPEQVIATVKASGLRGRGGAGFSTGTKWELTRAAE
                     GPTKYVICNADEGDPGAFMDRSTLEGDPHSVLEALAIAAFAIGAEAGFVYVRAEYPLA
                     VKRLRIAISEARERGLIGRNILGSPFSFELELRLGAGAFVCGEETALIASIEGKRGMP
                     RPRPPYPAQSGLWGKPTLINNVETLA"
     gene            complement(1094..1495)
                     /locus_tag="PLQ54_11295"
     CDS             complement(1094..1495)
                     /locus_tag="PLQ54_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS93890.1"
                     /translation="MPEGQKVLIIDDDVDFTEANRLALEASGFRVTVANDSKTGAELA
                     AQSPPDLVICDLMMEQLYSGFSVVQSLAAHHKTSAVPIIMVSAVTTSTGFRVDVDGKK
                     PEWLNVVEFVNKPIDPVLLAAKVAAVLAAKA"
     gene            complement(1513..>2581)
                     /locus_tag="PLQ54_11300"
     CDS             complement(1513..>2581)
                     /locus_tag="PLQ54_11300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOS93891.1"
                     /translation="DALRDAMSMLLAIVILVTTVGVVMSFWLSSRIAGPVVGLTQAAV
                     RVAQGELEYRLPEPGSAQRDEIRQLVTAFNQMVTALQQRDAQLRETLDDLRSTSDELR
                     RWNQNYLDTLEFITHELKNQVAAMKVNLLAVRDGYVGELTEEQYEALDDVSTAINRTE
                     EMILNYLNLSRIEKGELEVRAQPVAISSDILRPVLRELKGQFEEKRMTVEVHVPDDMV
                     VQADPALLQIVYENLLSNAAKYGRQGGTVRMTGRRINGWVELHTWNEGQGVSAERLEE
                     LFRKFSRLQPPGHQERGTGLGLYITREIVRKHGGEIRAESREGEWIDFIFTLPRHDML
                     LEEFPEDVADTAEALQTTLIQ"
BASE COUNT          412 a          908 c          780 g          481 t
ORIGIN      
        1 tgccagcgtc tcgacgttgt tgatcagcgt cggcttgccc cagagtccgc tctgggccgg
       61 atagggggga cgcggccgag gcatgccgcg tttgccctcg atggaggcga tcagggccgt
      121 ctcctcaccg cacacgaacg ccccggcgcc cagacggagc tccagctcga agctgaaggg
      181 gctcccgagg atgttccggc cgatcaggcc gcgttcgcga gcttcactga tcgcgatccg
      241 cagccgcttc accgcgaggg ggtactcagc ccggacatac acgaatccgg cctccgcccc
      301 gatggcgaag gcggcgatgg ccaacgcctc gagcaccgag tgcggatcgc cctccagcgt
      361 gctgcgatcc atgaatgcgc ccgggtcgcc ctcgtccgcg ttgcagatca cgtacttggt
      421 gggaccctcg gccgcgcggg tgagctccca cttggtgccc gtcgagaagc ccgccccgcc
      481 acggccccgc agccccgacg ccttgaccgt tgcgatgacc tgttcgggcg tcatctcgtc
      541 gagcaccttg gccagcgcct ggtagccgcc ggccgccagg tagtcgtcaa tactctcggg
      601 gtccacgcgc cccacattgc ggagggtgat ccggagctgc ctcgcgaaga agtccacgtg
      661 acgccactcg tagatgaagg cgctcaccga actcggatcg gcttcgggcg ccccatccgg
      721 caccggcagc atccactcgg taaccgggtc gccgcccagc agatcgcggt tgacgatagt
      781 ccgaaccttg tccgcgtcgg ccaccttggt gtacatgcgc gccggctgcc cgggcacacg
      841 cacctctacc aggggttcga ggttgcaccg accgacgcaa ccgacctggt gcaggtcgac
      901 gcgatcgccc agtcccgctt ccgcgatggc cgtctcgagc tgctgccagg tcttctcggc
      961 gcccgctgcc agcgcacacg tggcgatgcc gaccagtacc tgcgtcttct ccggcttccg
     1021 cgtcacgtgc ggccgtgctg cctgggcctg cttctggagt tcctgtgtcg cctgtcccat
     1081 cacgccatcc gtcctaagcc ttggctgcca acacggccgc cactttggcc gcgagcagaa
     1141 ctggatcgat cggtttgttc acgaactcaa cgacgttgag ccactcgggc ttcttaccat
     1201 ccacatcgac gcgaaatccc gtgctcgtgg tcacggccga gaccatgatg atcggcaccg
     1261 cgctcgtctt gtggtgcgct gccagagact ggaccacgga gaaacccgag tacagctgct
     1321 ccatcatcag gtcacagatg accagatccg gcggcgactg ggccgccaac tccgctccgg
     1381 tcttgctgtc gttcgctacc gtgacgcgaa agccgctggc ctcgagagcc aggcggttcg
     1441 cttccgtgaa gtccacgtcg tcgtcgatga tcaggacctt ctggccctcg ggcatgatcg
     1501 gtcacccgtc cctcactgga tcagcgttgt ttgcagtgcc tccgcagtat cggcaacgtc
     1561 ctcagggaac tcctcaagga gcatgtcatg ccgggggagc gtgaagatga agtcgatcca
     1621 ctcgccctcg cggctctccg ccctgatttc cccaccgtgc ttgcggacga tctcccgcgt
     1681 gatgtacagc cccagcccgg ttccccgctc ctggtgtccc gggggctgga gtctggagaa
     1741 cttccggaac aactcctcga ggcgctcggc ggagacgccc tgtccctcat tccatgtgtg
     1801 gagctcgacc caaccgttga tgcgccggcc ggtcattcga accgttccac cctggcggcc
     1861 gtacttggcc gcattgctca agaggttctc gtagacgatc tggagcagcg ccggatcggc
     1921 ctggaccacc atgtcgtcgg gcacgtgcac ctcgaccgtc atgcgcttct cctcgaactg
     1981 ccccttcagc tcccgcagca cgggccgcag gatgtcggag gagatggcca cgggctgcgc
     2041 gcgaacctcc agttcgccct tctcgatccg ggacaggttc aagtagttta aaatcatctc
     2101 ttctgtccgg ttgatcgccg tgctcacatc gtcgagcgcc tcatactgct cctcggtgag
     2161 ctcacccacg tagccatcgc ggacggccag caggttgacc ttcatggcag cgacctggtt
     2221 cttgagctcg tgggtaatga actccagagt gtccagatag ttctggttcc accgccggag
     2281 ttcgtcgctc gtgctccgaa ggtcgtcgag cgtctccctc aactgtgcat cccgctgctg
     2341 cagggcggtc accatctggt tgaaggccgt caccagctgt cggatctcat cccgttgggc
     2401 cgagcccggc tccgggagcc ggtactccag ctcgccctgc gccacccgga ctgccgcctg
     2461 ggtgagtcca accacgggcc ccgcgatacg actggagagc caaaaggaca tgacaacgcc
     2521 cacggtggtc accaggatca cgatggcgag cagcatgctc atggcgtcgc ggagcgcgtc
     2581 g
//
LOCUS       DAOSPL010000604         2580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40013_length_2580_cov_3.089901, whole genome shotgun sequence.
ACCESSION   DAOSPL010000604 DAOSPL010000000
VERSION     DAOSPL010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40013_length_2580_cov_3.089901"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..847)
                     /locus_tag="PLQ54_11305"
     CDS             complement(131..847)
                     /locus_tag="PLQ54_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93892.1"
                     /translation="MSLNHGFPFPVILKAHADARGCDAQRRAIAGHEPKPLQRCAGEE
                     PVVFRVTLGRTFRLDHRLQPRPDIPGAAGVEIRRDPPAEQDGLDGQVHQGSQLVLEGD
                     PVSPNTHRGPQTPLGRLRAVNDGVRLPVTGCLDCHVGGVGLVLVTVAAVPPGTDDRHT
                     QVAQEHGLAHAERRDLAARAHFGCELGRGSGRAAGQTHQRGHERESEHHGGSTSRWRS
                     LAEYVRLPFAAPLPALLRHS"
     gene            846..>2580
                     /locus_tag="PLQ54_11310"
     CDS             846..>2580
                     /locus_tag="PLQ54_11310"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HOS93893.1"
                     /translation="MTTVGSGRLAANEPVRLGLVLAADTAEGIAEDAQAIRESSLTGL
                     DMTDHRALKLGAVTLDRAEIDVYSHVEIRADLEATYENPFDPEDIRVDAEVTAPDGRV
                     RVVPGFYYVPLRLEMRGDSEALVRDGSPDFRVRFTPMEPGEHSLVLVVRDGDRTVRSE
                     PVRVTARPSGAPGFVRVAAASPRYFAFDNGQPYFAVGENVCWSWNDQALTHYRAWLGG
                     LGAAGGNWARLWLAFNEKGQEWMPQPTPKGGMGAYLGLGRYAQDNAWRLDEIIRMGEA
                     NGVYLMLCLGTFGEFTEGGYFNEGSWISNPYNAANGGPCATPGDFWTSPQARQLYQQR
                     LRYLIARWGYSTHVFAWEFWNEVSPTPDQVAWVAEMAAYLKQHDPTGRLVSTTYGDAT
                     TWACPDVDFTMSHMYGSAGNVADFTPMIASEVGNHRAYGKPYLLAEFGIDWQTYDGKW
                     DPKGTGLNMHNGAWAAALSGAAGTAMLWYWDGYVHPNDLYHILTPVRRFADTIDWAHT
                     AFEPLTSVSIELPPGTPETFSDLQVPSAIEWGATPSAEYTVRPDGEVEGGPVARTIGS
                     PFRADPKELNQE"
BASE COUNT          424 a          854 c          894 g          408 t
ORIGIN      
        1 gtagagctgc aagggcgaga acggcgcatg gctgggacca tcggctgcga accatgggca
       61 ggagacccac ctttctgtca cgaccctcgc gcgcatctgc acggttcgtc accgcgcctc
      121 gcccgccttc ctatgaatga cgcaggagtg cgggcagcgg tgcggcgaag ggcaaacgga
      181 catactcggc gagggatcgc caccgcgagg tgctgccgcc atggtgttcc gactcacgct
      241 cgtgtccgcg ctggtgagtc tggcccgctg ctcggccgga gccgcgcccg agctcgcagc
      301 cgaagtgggc ccgcgcggcg aggtcacgac gctccgcgtg ggcgagaccg tgttcctgcg
      361 cgacctgggt gtggcgatca tccgtcccgg gtgggacggc agctacggtg accaacacca
      421 ggccgacccc gcccacgtga cagtcgaggc aaccggtgac gggcagtcgt acaccatcgt
      481 tgacagcgcg cagccgcccc agtggcgtct gcgggccacg gtgcgtgttg gggctaacag
      541 ggtcgccctc gagtacgagc tgactcccct ggtggacctg cccatcgaga ccgtcttgct
      601 cagcgggcgg atcccggcgg atctccacgc cggccgcacc cggtatgtcg gggcgcggct
      661 ggagtctgtg atcgaggcgg aatgtccggc cgagtgtgac ccggaacact actggttcct
      721 ccccggcgca tcgctggagt ggcttgggct cgtggccggc gatggcacgg cgctgcgcat
      781 cacaccctcg ggcgtccgcg tgggccttca ggatgaccgg aaatgggaag ccgtggttca
      841 ggctcatgac aaccgtcggg tccggacggc tggcggccaa cgaaccggta cgcctcgggc
      901 tagttctggc cgcggacacg gccgagggta tcgccgagga cgcgcaggcc atcagggagt
      961 cgtccctgac cgggctagac atgaccgacc accgggcgct gaagctgggc gccgtcaccc
     1021 tcgacagggc cgagatcgat gtgtactcgc acgtcgagat ccgcgccgac ctcgaggcga
     1081 cctacgagaa ccccttcgac cctgaggaca tccgggtcga cgccgaggtc acggcacccg
     1141 acggaagggt gcgagtggtg cccgggttct actacgttcc cctacggctc gagatgcgcg
     1201 gcgacagcga ggccctggtt cgcgacggct cccctgactt ccgcgttcgg ttcactccca
     1261 tggagccggg cgagcacagc ctggtgctgg tggtgcggga cggcgaccgc accgtccggt
     1321 cggagccggt tcgggtgacc gcgagaccca gcggcgctcc cgggtttgtt cgcgtggccg
     1381 ccgcatcgcc acggtatttc gccttcgaca acggccagcc gtatttcgcc gtgggcgaga
     1441 acgtctgctg gtcgtggaat gatcaggcgc tgacccacta tcgggcgtgg cttggaggcc
     1501 ttggcgcggc gggcggcaac tgggcgcgtc tgtggctcgc gttcaacgag aagggacagg
     1561 agtggatgcc tcagccgaca cccaaaggcg gcatgggcgc ctacctgggg ctcggccggt
     1621 acgcacagga caacgcctgg cgtctggacg agatcatccg gatgggcgag gccaacggcg
     1681 tctacctcat gctgtgcctg ggcaccttcg gcgagttcac cgagggcggc tatttcaatg
     1741 agggctcgtg gatcagcaac ccctacaacg cggcgaatgg tggcccgtgc gccacgccgg
     1801 gggacttctg gacgagtcca caggcgcggc aactctacca gcagcgcctg cgctacctca
     1861 tcgcccgctg gggctactcg acccatgtgt tcgcgtggga gttctggaac gaggtgtcgc
     1921 ccacgccgga ccaggttgcg tgggtggccg agatggcggc ctacctcaag cagcacgacc
     1981 ccaccgggcg tctggtcagt actacgtatg gcgacgcgac cacgtgggct tgcccggatg
     2041 tggacttcac catgtcgcac atgtacgggt cggccggaaa cgtcgcggac ttcacgccga
     2101 tgattgcatc tgaagtcggc aaccaccgcg cctatgggaa gccctatctg ctggccgagt
     2161 tcggcatcga ctggcagacc tacgatggca agtgggatcc caagggcacg gggctgaaca
     2221 tgcacaacgg agcatgggcc gccgcgctgt caggggccgc cgggacggct atgctgtggt
     2281 actgggatgg ctatgtccat cccaacgacc tgtaccacat cctgacgccc gtccgtcgct
     2341 tcgccgacac catcgactgg gcgcacaccg cattcgagcc cctgacgagc gtgagcatcg
     2401 agctccctcc cggcacgccg gagaccttca gcgacctgca ggttccctcg gcgatcgagt
     2461 ggggggcaac accttcagcc gagtacacgg tgcgccccga cggcgaggta gagggcggtc
     2521 cggtggcccg gaccatcggt agccccttcc gcgccgaccc aaaggagctc aaccaggaga
//
LOCUS       DAOSPL010000605         2577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40075_length_2577_cov_2.476606, whole genome shotgun sequence.
ACCESSION   DAOSPL010000605 DAOSPL010000000
VERSION     DAOSPL010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40075_length_2577_cov_2.476606"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="PLQ54_11315"
     CDS             <1..1044
                     /locus_tag="PLQ54_11315"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HOS93894.1"
                     /translation="RLHVGSGGGRVSALFWRPLVLAVLVLAFGTFGYMCAEGWPFLDS
                     LYMTIITMTTVGFGEVRTLDPVGQEFTIVLVIVSVVVGALVLGTITSFVVEGHLHGAI
                     VTRRLRRRLSRLKDHIVVCGFGRMGMAAVRSALQQGATCVAIDEHPPDNLPDDLADVT
                     IICGDATVPEVLSEAGVERAAGVVCALDTDAENLMLALTLQEMAPDARVVARSTDEPS
                     EALLARAGADVVICPYRTAGFQMAAALLRPGVHSFLQVMAAGGSGSFRLNEVTVPLGS
                     HLIGMSLAEMQEETQTDVRIVGVGKPWGEPIINPSGSTIISEGDQLILVGTEEQVNRL
                     LRAVERPARPSER"
     gene            1073..1288
                     /gene="tatA"
                     /locus_tag="PLQ54_11320"
     CDS             1073..1288
                     /gene="tatA"
                     /locus_tag="PLQ54_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOS93895.1"
                     /translation="MLRILTDSVLGVGFVGSSELLIILLLVVLLFGARKIPELMRGLG
                     TGIKEFKKSSQDPPEPTEGPKDDAPTG"
     gene            1331..1720
                     /gene="queD"
                     /locus_tag="PLQ54_11325"
     CDS             1331..1720
                     /gene="queD"
                     /locus_tag="PLQ54_11325"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOS93896.1"
                     /translation="MYELAVEDHFHSAHRVDGYSGKCARLHGHTYLVRAVVAGDELDS
                     LGMLVDFATLRACLKRVLDELDHQLLNDLPLPELAPNPTAEVIARAVHARLASELAAD
                     GAVPSTVRLARVEVSESPGAVAVFIGN"
     gene            1770..2567
                     /locus_tag="PLQ54_11330"
     CDS             1770..2567
                     /locus_tag="PLQ54_11330"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOS93897.1"
                     /translation="MADTELESIAEPDARRDPFTLPVDEIFSGIQGEGPYVGIRQVFV
                     RLCGCNLMCEYCDTRRSRRADLATCTVLRPSSEPRVYPNPISLANMVDLVRETEAAAG
                     PHHSVALTGGEPLLHAGPLRPLVGHLGSLGFSCYLETNGTLPEGMRLLAPVVSWIAMD
                     IKLPSATGEPWRDADHLLCLREACSAEVFVKMVVVQKTPLGEVLQGCAIVNEVDPSIP
                     CILQPATGPDGGLLIDPQRLWVLAQAAAREVRDVRVIPQCHRFLGLA"
BASE COUNT          399 a          875 c          840 g          463 t
ORIGIN      
        1 cggctgcatg tcggatccgg cggaggccgc gtttccgccc tcttctggcg cccattggtc
       61 ctggccgtgc tggtcctggc cttcggtacc ttcggttaca tgtgcgccga gggctggccc
      121 tttctggatt cgctgtacat gacgatcatc acgatgacca ccgtcgggtt cggcgaggtc
      181 cgaacgctgg atccggtcgg gcaggagttc accatcgtgc tggtcatcgt cagcgtcgtg
      241 gtgggcgctc tagtgctggg caccatcaca tcattcgtcg tcgagggtca cctgcacggg
      301 gccatcgtga cgcggaggtt gcggcggcgc ttgtccaggc tcaaagacca catcgtggtg
      361 tgcggcttcg gtcgaatggg aatggccgca gttcgctcgg cgctccagca aggagccacg
      421 tgcgtggcca tcgacgagca cccgcccgac aacttgcccg acgacctggc cgatgttacc
      481 atcatatgcg gcgacgccac cgttcccgag gtcctctctg aggccggtgt cgagcgagcg
      541 gcaggggtgg tgtgcgcgct ggacaccgac gccgagaacc tcatgctcgc cctcacgctt
      601 caggagatgg ctcccgacgc gagggtcgtg gcacggtcca ccgacgagcc gtcagaggct
      661 cttctggccc gagccggcgc tgatgtggtg atctgcccct accggaccgc aggctttcag
      721 atggcggccg ccctgcttcg accgggcgtc cactcgtttc tgcaggtcat ggccgcgggg
      781 gggtcgggct ccttccggct gaatgaggtt acggttccct taggctccca cctcattggc
      841 atgtcacttg ccgagatgca ggaggagacg cagaccgacg tccgcatcgt cggggtcggc
      901 aaaccctggg gcgagccgat catcaaccca tccggctcca ccatcatctc cgaaggagac
      961 cagctgatcc tcgtcggcac ggaggaacag gtcaaccggc tcctccgcgc tgtcgagaga
     1021 ccggctcgcc cctcggagcg ctgacggggc gttcgcccaa ggaggcaacc caatgctacg
     1081 catcctgacc gactccgtcc tcggcgtcgg cttcgtgggc tcgagcgaac tcctgatcat
     1141 cctgcttctc gtggtcctgc tgttcggcgc gcgcaagatc ccggagctca tgcggggact
     1201 gggcaccggc atcaaggagt tcaagaagag ctcgcaggat ccgccggagc ccacagaggg
     1261 ccccaaagac gatgccccaa cgggctgatc gatgctgggc gaaagggagc ttcggctccg
     1321 tccggaggct atgtacgaac tggcggtcga agatcacttc cactcggccc atcgggtcga
     1381 tggctactcg gggaagtgcg cccggttgca tgggcacacc tacctcgtgc gcgccgtcgt
     1441 cgctggcgac gagctcgact ccctgggcat gctggtcgac ttcgcgacgc ttcgagcctg
     1501 cctcaaacgc gtcctcgacg agctggacca ccagctgctg aacgaccttc cgctgccgga
     1561 gctggcgccg aaccccacgg ctgaggtcat cgcccgagcc gtgcacgccc gtctcgcctc
     1621 cgagctggcg gcggacggcg cggttccgtc aactgtccgg ctcgcgcgtg tcgaggtctc
     1681 ggagagccca ggagccgtgg cggtgttcat cggcaactag gaggttccac cgccgtgggg
     1741 agcgccatgg cgatggcggt agggggcgta tggctgacac tgagctcgag tccatagccg
     1801 aacccgacgc gcgacgcgac cccttcactc tgcccgtgga cgagatcttc agcgggattc
     1861 agggggaggg gccatatgtc ggcatccggc aggtctttgt ccgcctttgc ggatgcaacc
     1921 tcatgtgcga gtactgcgac acgcgccgct cccgacgggc cgacctggcg acttgcacgg
     1981 tgctccgacc ctcttccgag ccacgcgtat accccaaccc catcagcctc gcgaacatgg
     2041 tcgacctggt gcgcgagact gaggccgccg ccggccctca ccactccgtc gcgctgaccg
     2101 ggggcgagcc actacttcac gctggcccgc tgcgcccgct cgtgggtcac ctcgggtcac
     2161 taggatttag ttgctatctt gaaacaaacg gaacgctgcc cgagggcatg cgactcctgg
     2221 cacctgtcgt gagctggatc gccatggaca tcaaactgcc gtccgccacc ggcgagccct
     2281 ggcgcgatgc cgaccacctg ctctgcctcc gcgaggcctg ctccgccgag gtgttcgtca
     2341 agatggtcgt cgtgcagaag acccccctgg gcgaagtgct ccagggctgc gcgatcgtca
     2401 atgaggtcga tccgtcgatc ccgtgcatcc tgcaaccggc gaccggccct gatggtgggc
     2461 tgctcatcga cccccagcgg ctctgggtcc tggcgcaggc ggccgcccgg gaggtgcggg
     2521 atgtccgggt catccctcag tgccaccggt tcctcggcct ggcctagcgt gacaacg
//
LOCUS       DAOSPL010000606         2576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40091_length_2576_cov_3.054740, whole genome shotgun sequence.
ACCESSION   DAOSPL010000606 DAOSPL010000000
VERSION     DAOSPL010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40091_length_2576_cov_3.054740"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="PLQ54_11335"
     CDS             <1..1446
                     /locus_tag="PLQ54_11335"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOS93898.1"
                     /translation="AVIHGPAPLGPGRLVLNGAGDGEVWLSRVRPGESLDIALECPGL
                     PEWAAGAIGGGPLLVRHGLIVGGDAVRHPRTAVGYNHRETIWVTVDGRRAGWSVGMTL
                     TELAHLMRELGCTDAMNLDGGGSTTLWVRGLVVNRPSDGQPRPVANGLAVVLSRPVGP
                     PAHIRATPSEVWGLPSGEVLPELLVTDSQYNPLPVGAGSLKLSAEGAAALGPDGRTIR
                     LAAAPGEGRVHVSRGAARATVPVHVLAAPAAARIVPGTATAMPGDVLSFELELLDAEG
                     HVLVAPPGRERWVCGSGLALEGDTGRVAVTDDAASGDVHCFSHGISASARIEAARRLV
                     VASGQPGERLAFRAVPDGPGGPSGAVRVDDGRLVLDFDLGGRAGTRAAYAAVDRDVGH
                     CIGFTVAARAEGCSPWVRVAYRDGNGTRQTASLSDRLPDGGPWQTLRLRLPDGVKAPV
                     TLESVYVVETEPAVTARGTLLLDDLCAWVLE"
     gene            complement(1451..>2576)
                     /locus_tag="PLQ54_11340"
     CDS             complement(1451..>2576)
                     /locus_tag="PLQ54_11340"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOS93899.1"
                     /translation="AACTLLDNYVSGFDDFYPPEKYPRDPLLRRFYAFIQDHRIDVSR
                     VGFRHLKIERPEGGEVKLDFTEADPYIEFCVDRGLQCLDVFSSAGAPYGGAEVRQWSE
                     HLKRKGWHDKAFAYALDEPAPEAFAEVVARAKAIQEADPTIPRMCTTPPNETMEGLIN
                     VWCPLTPHITPEFVAERHAKGDLIWSYVCIGPKKPPANFFIDYPATDPRVLFWWAYKC
                     GLDGFLYYAMDTWQIYGKNYPATLPDFAAAAEGKAWGLPHFNPLTWADGNGDGLLMYP
                     GPDGPWSSLRLEIIRDGIEDYEYLATLRDRLGDLKRLDGGNAYTVLRSRIERVLAVSD
                     GICANVNEYTDDPQRILDARQEAAEVLEDATAAVAVLRRR"
BASE COUNT          357 a          871 c          914 g          434 t
ORIGIN      
        1 gccgtcattc acgggcccgc tcccctcggc ccgggccggc tcgtcctcaa cggcgccgga
       61 gatggggagg tctggctcag tcgcgtgagg cccggcgaga gcctcgatat cgcgctggag
      121 tgccccggtc tgcccgagtg ggccgccgga gctatcggag ggggcccgct tctggttcgc
      181 catgggctca tcgtcggagg ggacgccgtt cgccatccac gtacggccgt ggggtacaac
      241 caccgcgaga ccatctgggt cacggtcgat ggccgccgtg ccgggtggag tgtggggatg
      301 acgctcaccg agctcgccca cctgatgcgc gaactcggct gcaccgatgc catgaacctc
      361 gacggcggcg gttccaccac cctgtgggta cgaggcctgg tggtgaaccg gccgtcggac
      421 ggccagccgc ggcccgtcgc caacggcctg gccgtggtgt tgagtcgacc cgtgggcccg
      481 cccgcccaca tccgcgctac cccgagcgag gtctgggggc tgccgtctgg cgaggtgctg
      541 cccgagcttc tggtgaccga ctcccagtac aacccgctgc cggtcggggc cggtagtcta
      601 aagctgtcgg ccgaaggtgc ggctgcgctg ggccccgatg gccggaccat ccgcctcgcc
      661 gcggccccag gtgagggtcg tgtgcacgtg tcgcggggcg ctgcccgcgc gaccgtgccc
      721 gtgcacgtgt tggccgcgcc ggccgccgct cgcattgtgc cgggcaccgc caccgcgatg
      781 cccggcgatg tgctctcatt cgagctggag ctgctcgatg ccgaggggca cgtcctggtg
      841 gcgccgccgg ggcgggagcg ctgggtctgc ggttctggtc ttgcgctcga gggtgacacg
      901 ggccgcgttg cggttacgga cgatgccgcg tcgggtgacg tccactgctt ctcacacggc
      961 atctcggcct cggcgcgcat cgaggcggcc cggaggctgg tcgttgccag tggtcagccg
     1021 ggtgagcgcc tcgcgttccg tgccgtaccg gacggtccgg gtgggcccag tggcgcggtg
     1081 cgggtcgacg acggccgcct ggtgctcgac ttcgacctcg gtgggcgggc cgggacgagg
     1141 gcggcctatg cggccgtcga ccgcgatgtc ggccactgca tcgggttcac tgtggccgcg
     1201 cgcgccgagg ggtgctcgcc gtgggtgcgg gtggcctacc gcgacggcaa cggcacgcgc
     1261 cagacggcat ccctgtccga tcggctgccc gacggcgggc cctggcagac gctccggctc
     1321 cggctgccgg acggcgtgaa ggcccccgtg accctcgaat ccgtctacgt tgtcgagacg
     1381 gagccggcgg taacggcccg aggcacgctt ctccttgatg atctgtgtgc ctgggtgctc
     1441 gagtagcgac ctatcggcgc cgcagcacgg ccactgccgc ggtggcgtct tcgagcactt
     1501 ccgcagcctc ctgacgcgca tccaggatgc gctgggggtc atccgtatac tcgttcacgt
     1561 tggcgcaaat cccgtcgctg accgccaaca cccgctcgat cctcgaccga agcaccgtat
     1621 aggcgttgcc gccgtccagc ctcttcaggt cacccagccg gtcgcgcagc gtggccaggt
     1681 actcgtagtc ctcgatgccg tcacggatga tctcgaggcg cagactcgac catggcccgt
     1741 cgggtccggg gtacatgagc aggccgtcgc cgttcccgtc ggcccaggtc agtgggttga
     1801 agtgagggag gccccaggcc ttcccctcgg ccgccgccgc gaagtcgggc agtgtggcgg
     1861 gatagttctt cccgtagatc tgccaggtgt ccatggcata gtacaggaag ccgtcgagac
     1921 cgcacttgta ggcccaccag aacagcacac gcgggtcggt cgccgggtag tcaatgaaga
     1981 agttcgcagg cggcttcttg gggccgatgc agacatagga ccatatcaga tcgcccttgg
     2041 catggcgttc ggccacgaac tcgggcgtga tgtgcggagt caagggacac cagacgttaa
     2101 tgaggccttc catggtttcg ttggggggcg tggtacacat ccgcggaatg gtcggatcgg
     2161 cttcctggat cgccttggcg cgcgcgacga cctcggcgaa cgcctcgggt gccggctcat
     2221 ccagcgcgta ggcgaacgcc ttgtcatgcc agcctttccg cttgaggtgt tcggaccact
     2281 gccgcacctc cgccccgcca tagggcgcgc ccgcgctcga gaacacgtcc aggcactgca
     2341 ggccccggtc cacgcagaac tcgatatagg ggtccgcctc agtgaaatcg agcttcacct
     2401 cgccgccctc cggacgctcg atcttcaggt ggcgaaatcc cacgcgggac acatcgatgc
     2461 ggtggtcctg gatgaacgcg taaaaccgcc ggagcagcgg atcgcgcggg tacttctcgg
     2521 gcgggtagaa gtcgtcgaag ccgctgacgt agttgtccag cagggtgcac gccgcc
//
LOCUS       DAOSPL010000607         2576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40098_length_2576_cov_2.457755, whole genome shotgun sequence.
ACCESSION   DAOSPL010000607 DAOSPL010000000
VERSION     DAOSPL010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40098_length_2576_cov_2.457755"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PLQ54_11345"
     CDS             <1..241
                     /locus_tag="PLQ54_11345"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOS93900.1"
                     /translation="PFIANELGWICAEVGRQPWAVYGMLRTSDAVSVVVPAGEILASI
                     LLISTIYAVLFAAWVLVLRRIMREGPEEPREAVAT"
     gene            238..1260
                     /gene="cydB"
                     /locus_tag="PLQ54_11350"
     CDS             238..1260
                     /gene="cydB"
                     /locus_tag="PLQ54_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOS93901.1"
                     /translation="MSNLEVTWFFLVGILLTAYAVLDGFDLGVGFWYLFTRSDAHRRV
                     LRTAIGPVWDGNEVWLLTGGGALFAAFPHAYATVFSGFYMALMLVLAALILRAASLEF
                     RKEMDSPGWRRVWDWAFGLGSTLPALLFGVALGNILRGVPLDADKQFTGTFFTLLNPY
                     ALLIGVAGLLMLLTQGALFIALKTEGSLSAQGRQWAKATGPGYVLLLLVAMAATRLLE
                     PQLLTNYADAPVLWVLPVLAVTLGAAALVLNLRRQESRAFVAWSACIVATMAMAGTGL
                     FPNIVPALDNPALSLTADNASSGPLTLAVMLVIAAVGMPFVLGYTVWIYRVFAGKVTV
                     GAADHY"
     gene            1308..2516
                     /locus_tag="PLQ54_11355"
     CDS             1308..2516
                     /locus_tag="PLQ54_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOS93902.1"
                     /translation="MASPYQAIRVSDHVYWVGAIDWAVRDFHGYLTQRGTTYNAFLVM
                     GDRIALIDTVRSAFFDEMMARIASVVDPSRIDVIVSNHAELDHSGGLRQAIAALNPTD
                     VHASDQGVKALIEHFGPIDNLTPVSEAQPLDLGNMGLSFIETRMLHWPDSMFSYLAED
                     QVLFCQDAFGMHLATSERFDDELPLDLLNYEAARYYANILMPLSTMVTKLLERYGKLG
                     LAVKYLATDHGPIWRSRLDHIMGLYGQWAAQAPSRKAVVAYETMWGSTSRMAHAVAEG
                     LIAQGVSTKVMKLGACHRSDIATEVLDAGALLLGTPTLNSTMLPQVADVLCYLRGLQP
                     KNLVGAVFGSHGWNGKGVDHARESLAAMGVELVGDAVRSKYVPDATTLDQCRALGESV
                     AHRLGQICLA"
BASE COUNT          391 a          911 c          798 g          476 t
ORIGIN      
        1 gccattcatc gccaacgaac tcggctggat ctgcgccgag gtcggacgcc aaccgtgggc
       61 cgtgtacggc atgctccgta cttccgacgc cgtctccgtg gtcgtgccgg ccggcgagat
      121 actcgcttcg atcctcctga tctcgaccat atacgcggtg ctcttcgccg cctgggtcct
      181 ggtcctccgg cggatcatgc gagagggccc tgaagagccc agagaggcgg tggccacgtg
      241 agcaacctcg aggtgacctg gttcttcctc gtcggcatcc tgctcacggc ctacgccgtg
      301 ctcgacggct tcgacctggg cgtcggattc tggtacctgt tcacgcggtc ggacgcacac
      361 cgtcgcgtgc tccgcactgc catcggaccg gtgtgggatg gcaacgaggt gtggctcctc
      421 accggcggcg gcgccctgtt cgccgcattc ccccatgcct acgccaccgt gttcagcggc
      481 ttctatatgg ctctgatgct cgtcctggcc gctctcatct tgcgtgccgc ctcactcgag
      541 ttccgcaagg agatggactc cccgggctgg cggcgagtct gggactgggc cttcggcctc
      601 ggcagtaccc tgccggcgct cctgttcggc gtggcactgg gcaacatact ccgaggggtg
      661 ccactggacg ccgacaagca gttcaccggc accttcttca ccttgctgaa cccctatgcg
      721 ctcctcatcg gcgtcgccgg gctgctcatg ctcctcacgc agggcgccct gttcatcgcc
      781 ctcaagaccg agggcagcct ctcggcccag ggccgccagt gggccaaggc taccggcccg
      841 ggctacgtgc tgctgctgct cgtggccatg gctgccactc ggttgctcga gcctcagctc
      901 ctcaccaact acgccgacgc gcccgtgctc tgggtgctgc ccgtgctggc cgtgacgctg
      961 ggcgcggctg ccctggtact gaatctgcgc cgccaggaat cccgggcctt cgtcgcctgg
     1021 agcgcctgca tcgtcgcgac catggcaatg gcgggcaccg gcctcttccc gaatatcgtg
     1081 ccggcactcg acaatccggc actgagcctc accgccgaca acgcatcgtc gggcccactc
     1141 acgctcgccg tcatgctcgt catcgccgcg gtcggcatgc ccttcgttct gggctacacc
     1201 gtctggatct accgcgtctt cgccggcaag gtcacggtcg gcgcggccga tcactactag
     1261 ctcgcgcggc cgcgcgccgc gttccccgga gggatcatca ccacaccatg gcgtccccat
     1321 accaggccat tcgagtcagc gaccacgtct actgggtcgg ggccatcgac tgggccgtcc
     1381 gtgacttcca cggctatctc acccagcgag gcacgaccta caacgccttc ctcgtgatgg
     1441 gcgaccgcat cgcgctcatc gacaccgtcc gatcggcctt cttcgatgag atgatggctc
     1501 gcatcgcctc cgtggtcgat ccatcccgca tcgacgtcat cgtctcgaac catgccgagc
     1561 tcgaccattc gggcggcctg cggcaggcca tagcagccct caatcccacg gacgtgcacg
     1621 cgtcggacca gggcgtgaag gccctcatcg agcacttcgg ccccatcgac aacctcacgc
     1681 ccgtctccga agcgcagccg ctggatctcg gcaacatggg cctgtctttc atcgagacgc
     1741 ggatgctcca ctggcccgac tcgatgttct catacctggc cgaggaccag gtgctgttct
     1801 gccaggacgc cttcggcatg cacctcgcca ccagcgagcg cttcgatgat gagttgcccc
     1861 tggacctgct gaactacgag gcggcccgat actatgccaa catcctcatg ccgctctcaa
     1921 ccatggtgac gaaactgctg gagcggtacg ggaagctggg gcttgccgtg aagtacctgg
     1981 ccaccgatca cggccccatc tggcggtcgc ggctcgacca tatcatggga ctctacggcc
     2041 aatgggcggc ccaggcgccc tcgaggaagg ccgtggtggc ctacgagacc atgtggggca
     2101 gcacttcgag gatggcccat gcggtggccg aggggctcat cgcccaaggc gtgagtacga
     2161 aggtcatgaa gctcggcgcc tgccaccgga gcgacattgc caccgaggtg ctcgacgccg
     2221 gcgccctgct gctcggcacg cctaccctca acagcaccat gctgccccag gtggccgatg
     2281 tgctgtgcta tctgaggggc cttcagccga agaacctggt cggcgcggtt ttcggctcgc
     2341 acgggtggaa cggcaaggga gtcgaccacg cccgggagtc gctcgcggcc atgggtgtcg
     2401 agctcgtggg agatgccgtc cggtccaagt acgtgcccga cgctaccacg ctcgatcagt
     2461 gtcgcgccct gggtgagtcg gtggcacacc ggcttggcca gatctgtctg gcctgaggga
     2521 gcgctccggc gctccgacga ccatcgtgag gagtggagcc atgaagtggg tatgtg
//
LOCUS       DAOSPL010000608         2575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40107_length_2575_cov_3.266270, whole genome shotgun sequence.
ACCESSION   DAOSPL010000608 DAOSPL010000000
VERSION     DAOSPL010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40107_length_2575_cov_3.266270"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(449..835)
                     /locus_tag="PLQ54_11360"
     CDS             complement(449..835)
                     /locus_tag="PLQ54_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOS93903.1"
                     /translation="MQFHHVQIALPAGQEEQARRFYSDALGLTEVGKPPDLAGRGGCW
                     FRHLDGEITTLEIHLGVEEPFTPARKAHPAILVDSVQALKDLGDRIDQAGFEVSWAER
                     STFAGYERFHCKDPFGNRIEVMAAST"
     gene            complement(998..1327)
                     /locus_tag="PLQ54_11365"
     CDS             complement(998..1327)
                     /locus_tag="PLQ54_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOS93904.1"
                     /translation="MLRIGAVVLNVADTDRAGSFWSEALRFDRGDNPDFLLPREGGAC
                     RLHLDSTDRTHLDLWTDSEGEQRAEIERLVSLGATRVEWDYPDNADFVVLADPTGTLF
                     CVINIDA"
     gene            complement(1622..2167)
                     /locus_tag="PLQ54_11370"
     CDS             complement(1622..2167)
                     /locus_tag="PLQ54_11370"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOS93905.1"
                     /translation="MSPPPESAAGQEGSSASGDVRTARLLVVGQFVLIGILVVLPNGD
                     DWPVPAALTVACSAATVLGLAVMVIGATGLGRGLTATPLPNAHAQLRTGGLYRYARHP
                     IYSGLLLMMASITVAAGSGLRLLTLAALVLVLNVKARWEETRLAQRFEAYADYAARTP
                     RFVPLRLRRPPREQVREVPGR"
BASE COUNT          417 a          822 c          877 g          459 t
ORIGIN      
        1 cgccgggggt cggcctcggg tcgtcgagca gtagtgagac aagatcactg cgtgggcgcc
       61 gacgtcgtct tcttcagcgg ccccagtgag gccgcggctg tcgaaggcga gcgtccggga
      121 ggcgactggg ctcggcctgt gcaggtgggc acccgcaatg cgggctggtt tcgaaacgga
      181 ggccgttctc gaaggagccg gggcggcatt cgacggcttc gcctcgcgcg gctacgacgg
      241 cgctgatcga agaagacgcc gcccaacgat tccagcgaga agaccgagcc cgcctgccgt
      301 gaagccgagc agtgcggaat ccggccacgt ccgggtcgtt ccacctgcgt ctctccgccg
      361 ggggcttggc gtcgccttcg cgattggcta ggccagcgtt cgccgccggt ccaattgctt
      421 ggataccggg aaccgtggtg gcgccggctc aggtcgaggc ggccattact tcgatgcggt
      481 tgccaaatgg gtccttgcag tggaagcgtt cgtacccggc gaacgtgctc cgctcggccc
      541 agctgacctc gaacccggcc tggtcgattc ggtctccgag gtccttcagc gcttggacgg
      601 agtcgaccaa gatcgcggga tgcgccttcc tcgcgggcgt gaaaggctcc tcaaccccga
      661 gatgtatctc gagagtcgtt atctcgccgt cgaggtgtcg gaaccagcat ccaccccgcc
      721 cagcgaggtc ggggggcttg cctacctcgg tcagtcccag cgcatccgag tagaagcgcc
      781 gggcctgttc ctcctgaccg gccggcaacg cgatctggac gtggtggaac tgcatgcagc
      841 ctcctcgatg tcttgatttc aagatagctg accttgtgtg ctccgaggac gtagcgttac
      901 atcgccggct gttcaccccg tctggcttcc gtccaacgtt cgccatcagc acgatgctgc
      961 cccagggtgc agaagagtgg gcactctcgc cggtgagcta ggcgtcgatg ttgatcacgc
     1021 agaacagggt gccagtcggg tcagccagca ccacgaagtc ggcattgtcc gggtaatccc
     1081 actcgacacg ggtggcaccg agagagacca atcgctcaat ctcggcacgc tgctcacctt
     1141 cgctgtctgt ccataggtcc aggtgagtgc ggtccgtgct gtccaagtgg aggcggcagg
     1201 caccgccctc gcggggcagc aagaaatctg ggttgtctcc acggtcgaat cgcagtgcct
     1261 cgctccagaa cgagccggcg cggtcggtat cggcaacgtt cagcacgacg gctccgatgc
     1321 gcagcatctc ctcaggttat tcgccgacag gactgagcgg taggaggtcg ggtaacttgg
     1381 cgaatgcacg gatctcccgc gtccgcagag gctcgctgcg ccgccaatac gtgtgtaagc
     1441 cctggcgcga attctgcgag cgcgacgctc cggcgccctt atgagcgaat cgccggcgct
     1501 ctcagggtga cgaatgtcgg cgttcggagc gactgcccga gcccgacgag attccccaac
     1561 cggttgacga cctgcgtcac agcgccggga gccgaccacg tggtctgtgg ttgcgcctcg
     1621 ctcaacggcc aggcacttcc cgcacttgct cccgcggtgg cctccgcaac cgtaagggca
     1681 cgaaccgtgg agtgcgggcg gcgtagtcgg cgtatgcctc gaatcgctgg gccaggcgtg
     1741 tttcctccca tcgtgctttc acgttcagga ccaggaccag cgctgcgagg gtgaggagcc
     1801 ggagcccgct tccggccgcc actgtgattg aggccatcat gagcagcaac ccgctgtaga
     1861 tggggtgccg tgcgtaccgg tagaggccgc cagtacgcag ctgtgcgtgg gcgttcggca
     1921 acggcgtggc cgtcagacct cggccgagtc ccgttgcgcc gatgaccatc acggccaggc
     1981 ctaggacggt ggccgcgctg caggcaacgg tcagagcggc gggcaccggc cagtcatcgc
     2041 cattcgggag caccacgagg ataccgatca gcacgaactg gccaacgacc agtagccgcg
     2101 cggtcctgac atcgccagag gccgagctgc cttcttgccc agcggcggat tctgggggtg
     2161 ggctcatctc gcgtaccgca gtagcatgcg cttctcgtcc gttcccccat ccacgataac
     2221 caccgtacgg ttcccagcgt gcgggaaggg cgtcagcgca accgggagat ggggcatacc
     2281 gccggaaatc gagcgcgtcg agtggtgcgt gcatgcgcca actgtggcgg gcctgtgcct
     2341 ctgtggtgtg gaggggccct cgggctcttc gttgggacgt tcggcatccc tcccacgcgg
     2401 gctggcagcg gatccggctc atgtttatga gcggattcgg atcgtcgttc acagttgcat
     2461 gtcggacgtt gtctgcgcga cgagagcgtc attaccggag tcgttggtgc ggggatccaa
     2521 ggacgaggcg ggcgcggtgt cgagcgcccg gccggccagg gccaggccga cgctg
//
LOCUS       DAOSPL010000609         2574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40127_length_2574_cov_3.144105, whole genome shotgun sequence.
ACCESSION   DAOSPL010000609 DAOSPL010000000
VERSION     DAOSPL010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40127_length_2574_cov_3.144105"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1840)
                     /locus_tag="PLQ54_11375"
     CDS             complement(<1..1840)
                     /locus_tag="PLQ54_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93906.1"
                     /translation="MRHRHSRGARATALSLISVLLFTLLGGCGGGNPVNPPAATSGIV
                     TTGASATSAAAAALGVGDGAAWQDSQSLTVPADSAFLFVSSCADQAGANLTGASVRLT
                     SPSGERTITLGESALAATGFTEADLASATTDPALEAYADSIAGELPAQPSDDDVAELL
                     SRLKNGDPEGTLPGLPDGLADQVDTYRETIDGLTNAIPASDTPPGPTDGLLVVGPEAG
                     TWTIEVDAEASAGAFLVLAWAVPADAEGLGIDAMSQGLAGAATAAGVRAGPAGPRYLG
                     ADGGDPGCVQCMNQFKSPAAYTAEWFRAKAIGAVWFCLKWAQMRALFTKFIDYMFAIV
                     NPTVMSAIGLTAAGLKEKAILTGVIVWNLLKIVPQKAEEVFWQYAFVMQSAENYCMVC
                     YLGLKTPYEGGLLKRYNINPPVIVMEPDYTLTVELCAFNIAKDALEPPAYWGFGGGEH
                     GAGHGSATWALTPSSVATLTPSGVHNSQCTIKSPKEPEILAARIEVTLGSQPALGHFG
                     LNIEEPEKQYTVHGQGGASSGFGVDDNLDILKGTQVLYTTGYGMSGTKGPAVFKAKKG
                     DVLTFQVRDTYGHCSGLQAIYISDEAGHTDLVHETWDQGCGHGGGDR"
BASE COUNT          392 a          874 c          904 g          404 t
ORIGIN      
        1 cccggtcgcc gccgccgtgt ccgcagcctt gatcccaggt ctcgtgtacc agatcggtgt
       61 gaccggcctc gtcgctgatg tagatcgcct ggagaccgct gcagtgcccg taggtgtctc
      121 gaacctggaa ggtgaggacg tcccccttct tggccttgaa cacggctggg cctttggtcc
      181 ccgacatccc gtacccggtg gtgtacagga cctgcgtgcc cttcaggatg tcgaggttgt
      241 cgtcgacgcc gaagccactg gacgcgccgc cctgtccgtg cacggtgtac tgcttctcgg
      301 gctcctcgat gttgagaccg aaatggccca gggcgggctg gctgcccagg gtcacctcga
      361 tgcgggcggc gaggatctcc ggctccttgg gtgacttgat cgtgcactgt gagttgtgca
      421 cgccagacgg ggtgagcgtt gccacggagc tgggggtgag cgcccaggtg gcggatccat
      481 ggccggcacc gtgttcccca cctccgaagc cccagtaggc tgggggttcg agagcatcct
      541 tggcgatgtt gaacgcacag agctccacag tgagggtgta gtcgggctcc atgacgatga
      601 cgggtgggtt gatgttgtac cgtttgagca gcccaccctc gtagggtgtc ttgagcccca
      661 agtagcacac catgcagtag ttctcggcgc tctgcatcac gaaggcgtac tgccagaaaa
      721 cctcctccgc cttctgcggg acgatcttga gcaggttcca gacgatgacg cccgtcagga
      781 tggccttctc cttcagcccg gccgcggtga ggccgatggc cgacatcacg gtggggttca
      841 cgatggcgaa catgtagtcg atgaacttgg tgaacagcgc gcgcatctgg gcccacttca
      901 agcagaacca cacggcgcca atggccttgg cgcggaacca ttctgccgtg taggccgcgg
      961 ggctcttgaa ctggttcatg cactggacac agccgggatc gccgccgtcg gcgccgaggt
     1021 agcgcgggcc ggcagggccg gctctcaccc ccgcggcggt tgcggcgccc gccaggccct
     1081 gcgacatggc gtcgatgccc aggccctcgg cgtcggccgg aacggcccac gcgagcacca
     1141 agaaggctcc agcggaagcc tcggcgtcaa cctcgatggt ccacgtgccc gcctccgggc
     1201 ccacgacgag gagcccatcg gtcggtcctg gcggcgtgtc ggacgccggg atcgcgttgg
     1261 tgaggccgtc gatggtctcg cggtaggtgt cgacctggtc cgcgaggcca tcgggcagcc
     1321 cggggagggt cccctcggga tcgccgttct tgagccgcga taggagctct gccacgtcgt
     1381 catcggacgg ctgcgcaggc agctcgccgg cgatggagtc ggcataggcc tcgagggccg
     1441 gatccgtggt agcggaggcc agatcggcct ccgtgaagcc tgtagcggcc agcgcgctct
     1501 cgccgagggt gatggtccgc tcgccgctgg gcgaggtcag cctcacgctc gcccctgtga
     1561 ggttcgcgcc ggcctgatcc gcgcaggagg agacaaacaa gaaggccgag tcggcgggaa
     1621 cggtgagcga ctggctgtcc tgccacgccg caccatcgcc aacgcccagg gctgccgccg
     1681 ccgcggacgt ggcggaagcc ccagtggtga cgatgccgct ggtggcggca ggcgggttca
     1741 ccgggttgcc gccgccacac ccgcccagaa gggtgaacag gagaacggag atgagtgaga
     1801 gcgcagtggc cctcgcaccg cgcgaatgcc tgtggcgcat gaacgctcgc ctccgaaggc
     1861 cacagccggt caaccgggcc tcccggcgcg tggccgtgat cacgcctgca gtgacggctc
     1921 gccgacgctt ggccggagga gcaggctcgc gaggccgcgg gatcggcgga gctctcgccg
     1981 cgcgtgtttg cacgatggtt ccccgttggc gacagaaacc ctccattccc gcgacagagc
     2041 ctccacgcca ccatcgggcc acgccgcttc cacaggctca ggggggagct ctcgggcgac
     2101 cggccggtcg cccttacgag gcggggccgt ggagcatctc ggggacaccg ccccaagtca
     2161 cgcgggaggc ggcgcagcac ctgcccccgc tggcccatcg agggtcgtcg aggggttcgg
     2221 gcggactgaa gtccgcgctg catggacttg ggtacgtatg cctactggct gcggcaggac
     2281 gaccgctgct cgggcgtgcc gccaacggtg tcatcctgag tgccgcgccc gcggcgcgaa
     2341 ggatctcggt cagaaccggc cacgcaccga aggcacgggg ctcgcacagc ccccgcagcg
     2401 actgaaccac ggatcgaaac cgcggcatct ggcgctacca ttggccgtga ccggtccgtg
     2461 gcgcggccgt ctggcctgcg gcccgcccct ggcccggtag acccgccggg gagtcggccg
     2521 caggccgacg acgttcccca ccgccgcgca cgacccccaa ccccgtcggc cctg
//
LOCUS       DAOSPL010000610         2574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40129_length_2574_cov_2.954347, whole genome shotgun sequence.
ACCESSION   DAOSPL010000610 DAOSPL010000000
VERSION     DAOSPL010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40129_length_2574_cov_2.954347"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PLQ54_11380"
     CDS             complement(<1..635)
                     /locus_tag="PLQ54_11380"
                     /inference="COORDINATES: protein motif:HMM:NF017215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HOS93907.1"
                     /translation="MTDRETGPLVLGVDTGGTYTDAVLVSPTLGPVSWAKALTTPEDL
                     SRGIMEAIDGLDAGLRQRVVRVCLSTTLATNAVVEGRGARVFLFLLGHDESLIERFSL
                     ADALPGVEFAVVRGRMSQQGEEQEALDTDRLAQLVREKLPATDAFAVSGYFAVKNPAH
                     ETAARELIASICDRPVVCGHELSAQLDCILRAATCVLNARLLPLVRSFVAGV"
     gene            complement(628..1047)
                     /locus_tag="PLQ54_11385"
     CDS             complement(628..1047)
                     /locus_tag="PLQ54_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS93908.1"
                     /translation="MPDGRVLVVDDERPIVMVIETNLKISGYTVMTAYNGRDALELVR
                     TRRPDLVLLDVIMPEMDGWETLRRIREDSNPDVAKTPVVMLTALDRDRDIVRGYEYGV
                     DIYLTKPFEPDELLDIVARVLEAKAEGEFGDDSADDD"
     gene            complement(1195..>2574)
                     /locus_tag="PLQ54_11390"
     CDS             complement(1195..>2574)
                     /locus_tag="PLQ54_11390"
                     /inference="COORDINATES: protein motif:HMM:NF021347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HOS93909.1"
                     /translation="ELPRIVRHAVLFDPESSDHLSEHVEAAVNQAALAAQLEDRGLVA
                     FIADGSVLPRESGTSQLPMDPDRVVLFESCDEFRVSLELPSGDSITGMGIPEGVTLIV
                     GGGYHGKSTLLDAIQTGIYTHIPGDGRECVVTIRDTAKIRAEYGRRVAGVDISPYIRS
                     LPDGTATSAFSTACASGSTSQAANIQEALETGVRLLLIDEDTSATNFMVQDARMQRLV
                     AEESEPIVPFVDAVQSLHRDRGVSTILVMGGCGDYLDVADTVILMEGYLPKDARSRAR
                     EVVRELPSLRTRRAVSLPPAPRPRVPDPASIDASRGSHERRIGTHGMDYILFGEHEIS
                     FRQIEMLVDPSQLRAIGEAIEYAKRSGVLDGRHSLAEIAQWVEDRLMAEGVECVSPDV
                     GRHPGRLASFRRHDLAAAINRLPTLALVAEPGQVPRPPAGEPGGEAPGPAPAPASSKR
                     RRRRGLRRR"
BASE COUNT          399 a          895 c          894 g          386 t
ORIGIN      
        1 acccccgcca cgaagctccg gaccagcggc aggagtcggg cgttgagcac gcatgtggcc
       61 gcccggagga tgcagtcgag ctgggcggac agctcgtggc cgcagaccac cggccggtcg
      121 cagatcgagg cgatgagctc gcgcgcggcc gtctcgtgcg cgggattctt gacggcgaaa
      181 tagcccgaga cggcgaacgc atcggtggcc ggcagcttct cgcgaaccag ctgggccagc
      241 cgatcggtat cgagcgcctc ctgctcctcg ccctgctggc tcatgcggcc gcggaccacg
      301 gcgaactcca cgccgggaag agcgtcggcc agagagaagc gctcgatcag actctcgtcg
      361 tgacccagga ggaacaggaa cacgcgcgcc ccgcgcccct cgaccacggc attcgtcgcc
      421 agtgtggtcg agaggcagac ccgcaccact cgctgccgca ggcctgcgtc gaggccatcg
      481 atggcctcca tgatgccccg gctgaggtct tcgggtgtgg tcagcgcctt ggcccagctc
      541 acgggcccaa gggtggggga caccaggacg gcgtccgtat atgtgccacc cgtatcgact
      601 ccgagcacga ggggaccggt ctcgcggtca gtcatcgtcg gcggagtcat ccccgaactc
      661 gccctcggcc ttggcttcga gcacgcgggc gacaatgtcg aggagctcgt cgggctcgaa
      721 aggcttggtg aggtagatgt cgactccgta ttcgtagccc cgcacaatgt ctcggtcgcg
      781 gtccagcgcg gtgagcatga ccacgggggt cttggcgacg tcgggattgg agtcctcgcg
      841 gatccgacgc agcgtctccc acccgtccat ctcgggcatg atcacatcga ggagcaccag
      901 atcgggccgc cgggtccgga cgagctccag cgcgtcgcgg ccgttgtacg ccgtcattac
      961 ggtgtacccc gagatcttga ggttcgtctc gatgaccatc acaatcggac gctcgtcatc
     1021 gactaccagg actctgccgt ccggcacgtc acacaccccc tggtccggag ggccggggac
     1081 ttcccatacc ctcgggccac ggcccacgcc gcggccccac acccagacac ccggcccccc
     1141 ggggggttct cgcccccagg agtgccggga cccacgcaca atagtctaac agttctagcg
     1201 tcgccggagc ccccgacggc gccggcgctt tgacgatgcg ggcgccggcg cgggcccagg
     1261 cgcctcgccc ccaggttcgc ccgcgggggg ccggggcacc tgcccgggct cagcgaccaa
     1321 cgcgagggtc ggtagccggt tgatggctgc ggccaggtcg tgacggcgga acgacgcgag
     1381 ccgccccggg tgccgcccca cgtcgggcga gacgcactcc accccttcgg ccatgagccg
     1441 gtcctcgacc cactgagcga tctcggccag cgagtgtcgg ccgtcgagca caccggaacg
     1501 cttggcgtac tcgatggcct cgccgatggc ccgaagctgg ctcgggtcca ccagcatctc
     1561 gatctggcgg aaggagatct cgtgctcccc aaagaggatg tagtccatgc cgtgggtgcc
     1621 gatgcggcgc tcgtgcgacc cgcgactggc gtcgatgctg gccgggtcgg gcacccgcgg
     1681 gcggggtgcg ggcggaaggg acacggcccg gcgcgtccgc agtgacggga gctcgcggac
     1741 cacctcccgg gcgcgcgacc gcgcgtcctt ggggaggtac ccctccatca ggatcacggt
     1801 gtcggccaca tccagatagt cgccacaccc gcccatgacg aggatcgtcg acacgccgcg
     1861 gtcccggtgg agcgactgga cggcgtcgac gaagggtacg atgggctcgg actcctccgc
     1921 aaccaggcgc tgcatccgcg catcctggac catgaagttg gtggccgagg tgtcctcgtc
     1981 gatcaggagc agccggactc ccgtctccag cgcctcctgg atgttggcgg cctgcgaggt
     2041 gctcccggac gcacaggccg tcgagaacgc gctggtggcc gtgccgtcgg ggagcgatcg
     2101 gatgtagggg ctgatgtcga ctccggccac ccgcctgccg tactcggccc ggatcttggc
     2161 cgtgtcacgg atggtcacca cgcactcgcg cccatcgccg gggatgtgtg tgtagatacc
     2221 ggtctggatg gcgtcgagca gcgtcgactt gccgtggtac cctcccccga cgatgagggt
     2281 gaccccttcg gggatgccca tgccggtgat cgaatcaccg ctcgggagtt cgagggacac
     2341 ccggaactca tcgcacgact cgaacagcac cacgcggtcg gggtccatgg ggagctgact
     2401 ggtcccgctc tcgcgcggga ggacggaccc gtcggcgatg aaggccacca ggccccggtc
     2461 ttcgagctgt gcggccagcg ccgcctggtt cacggcggcc tcgacgtgct cactgaggtg
     2521 gtccgagctc tcggggtcga agagcacggc gtggcgcacg atgcggggca gttc
//
LOCUS       DAOSPL010000611         2573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40151_length_2573_cov_3.383241, whole genome shotgun sequence.
ACCESSION   DAOSPL010000611 DAOSPL010000000
VERSION     DAOSPL010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40151_length_2573_cov_3.383241"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLQ54_11395"
     CDS             complement(<1..296)
                     /locus_tag="PLQ54_11395"
                     /inference="COORDINATES: protein motif:HMM:NF013907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93910.1"
                     /translation="MNDLSDWSVVIGRVRTAYGIRGQLKVLPLCDDPQRFAQLTDVCV
                     AFADGRREMASVVSVTIQGDEIRLALRGVEDRTAAEGYRHAELRVRPEMRVPLP"
     gene            complement(293..709)
                     /locus_tag="PLQ54_11400"
     CDS             complement(293..709)
                     /locus_tag="PLQ54_11400"
                     /inference="COORDINATES: protein motif:HMM:NF022519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlqD family protein"
                     /protein_id="HOS93911.1"
                     /translation="MPSLIVRRPVGILRIVTEDFKSKLIADLKEAAESVEMRIQQIDF
                     QTRGYIEQVQKSDIKRAMALKEQVAAEKRRHESLRGEILERLQEAEALELDSEYEQGH
                     VESEVEIKEGDNITEVLGDAAIVVKDDVVIEIRGVK"
     gene            complement(851..1072)
                     /locus_tag="PLQ54_11405"
     CDS             complement(851..1072)
                     /locus_tag="PLQ54_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013370648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOS93912.1"
                     /translation="MVAFIARQLVDSPDDVSVMRVEQDRSVVYQLRVGPEDIGKVIGK
                     GGRIANALRQIVRAASSRSRRKCTVEIAS"
     gene            complement(1176..1463)
                     /gene="rpsP"
                     /locus_tag="PLQ54_11410"
     CDS             complement(1176..1463)
                     /gene="rpsP"
                     /locus_tag="PLQ54_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOS93913.1"
                     /translation="MVRIRLRRTGSRNAPAYRVVVADARAPRDGRFIENLGHYNPLTD
                     PAEVVIDKDRALYWLGVGAQPSETARSLLRQEGILQAFEEAKSRAHETPSE"
     gene            complement(1536..>2573)
                     /locus_tag="PLQ54_11415"
     CDS             complement(1536..>2573)
                     /locus_tag="PLQ54_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOS93914.1"
                     /translation="ARMMAKRGGTPLLVATDTRRPAAIRQLEVVAEQVGVPFFQMGDQ
                     LEPARIAAAAVSHARSHNLDTVIIDTAGRLHTDAEMMDEIRAVHQAVSPHETLLVLDS
                     LTGQDAVNVATEFCGTVDVDGVVLTKLDGDARGGAALSLRAVTGKPIKFAGVGEKFDA
                     LETFHPERIASRILGMGDVLTLIEKAQETVDQDKAVELQKKVLQDRFDLEDFLEQLQQ
                     MKRVGPLNQILEMLPKSWLGWAGALEDEEVDPKELDRIEAIIRSMTPDERHNPGLLNG
                     SRKKRIARGSGTTVADINSLLKQFDETQVMMKQMMGAQQGMAGRGLHGARPMRRKSAR
                     ATKSKKRSRSR"
BASE COUNT          417 a          907 c          766 g          483 t
ORIGIN      
        1 tcgggcaaag gcacccgcat ctccgggcga acccgcagct ccgcgtgccg gtatccctcg
       61 gcggcggtcc ggtcctcgac tccccggagt gccagtcgga tctcgtcgcc ctggatcgtg
      121 acgctcacca ccgaggccat ctcgcggcgg ccgtcggcga aagccacaca cacgtcggtg
      181 agctgagcaa accgttgggg gtcatcgcac agaggcagga ccttcagctg gcctcggata
      241 ccgtaggccg tccggactcg gccgatgacc accgaccagt cgctcagatc attcacttca
      301 cgcctcggat ctcgatgacc acgtcgtcct tgaccacgat ggcggcatcc cccagcacct
      361 cggtgatgtt gtcgccctcc ttgatctcga cctcgctctc aacgtggcct tgctcatact
      421 ccgaatccag ttcgagtgcc tcggcctcct gcagccgctc gagaatctca ccacggagcg
      481 actcgtggcg tcgcttctcg gcagccacct gctccttgag cgccatggcg cgcttgatat
      541 cgctcttctg gacctgctcg atgtaaccac gggtctggaa gtcgatctgc tggatgcgca
      601 tctcgaccga ctcagccgcc tctttgaggt cggcgataag cttgctcttg aagtcctccg
      661 tcacgatccg aaggatcccc accggtctcc gaacaatcag gctgggcatc ttggcttgtg
      721 ctcctctggg caagtctgtc ttccggcgaa cgcatgccat ccggacggag gcatgcgtcg
      781 aagcgccggt gggtccctcc gcaagccatg gcgcccccct gcacgggcga ggccgatgcg
      841 gaaggcaggg ctacgaggcg atctcgaccg tgcacttcct gcgggatcgg ctggacgcgg
      901 cgcgcacgat ctggcgaagg gcgttggcga tgcgcccccc cttgccaatc accttgccga
      961 tgtcctcggg tcccacgcgc agctgatata ccacggaccg gtcctgctcc acccgcatca
     1021 cgctgacatc gtccgggctg tccaccagct gccgggcgat gaaagcgacc agctcctcga
     1081 acggaggaac atcggcggcg tccgaagccc cagccgcgtc acaatccgcc gacgctggcg
     1141 agggttgcgc ggacggatcc cgcggcaccg gccgactact ccgacggcgt ctcatgggct
     1201 ctcgacttgg cctcctcaaa tgcctggagg ataccctcct ggcgaaggag cgaccgtgcc
     1261 gtctccgacg gctgggcccc cacaccgagc cagtacaaag cccgatcctt gtcgatgacg
     1321 acctctgccg gatcggtcag tgggttgtag tgtccgagat tctcgatgaa ccgaccgtcg
     1381 cgcggagctc gagcgtccgc caccaccacc cgataggccg gcgcattgcg gctccctgtc
     1441 cgccgcaggc gaatcctcac catttgcacg tgcctcccag cactctcacg gatgcgagcc
     1501 actacaggtg gcgtcgaccc gacaacgggc cgcgtctatc tggagcggct tcgcttcttg
     1561 gacttcgttg cacgagcgct cttgcgccgc atgggtctcg cgccatggag gccccggccc
     1621 gccatgccct gctgagcccc catcatctgc ttcatcatga cttgggtctc gtcgaactgc
     1681 ttgagcaggg agttgatatc ggcgaccgtg gttccgctgc cgcgcgcgat gcgcttcttg
     1741 cggctgccgt tcagcagccc cggattgtgt cgctcgtcgg gagtcatcga tcggatgatg
     1801 gcctcaatcc gatcgagctc ctttgggtcc acttcctcgt cctccagggc cccggcccac
     1861 ccaagccagc tctttggaag catctcgagg atctggttga gcggtccgac gcgcttcatc
     1921 tgctggagct gctcaaggaa atcctcgagg tcgaagcggt cctggagaac cttcttctgg
     1981 agctcaaccg ccttgtcctg atcgacggtc tcctgggcct tctcaatcag ggtgagtacg
     2041 tcacccatac ccaggatgcg cgacgcgatg cgctccgggt gaaacgtttc gagcgcgtcg
     2101 aacttctcgc ccaccccggc gaacttgatg ggcttcccgg tcacggcgcg tagtgagagg
     2161 gccgcgccgc cgcgggcgtc gccatcgagc ttcgtgagca cgacaccatc aacatccacc
     2221 gtgccacaga actcggtagc cacgttcacg gcgtcttgac cggtgagcga gtccagcacg
     2281 agcagcgttt cgtggggcga taccgcctga tgcaccgctc ggatctcgtc catcatctct
     2341 gcgtcggtat gaagccggcc cgccgtgtcg atgatgacgg tatccaggtt gtgcgaccgg
     2401 gcatgggaca cagcggccgc cgcgatacga gccggctcca actggtcgcc catctggaag
     2461 aacggcaccc caacctgctc ggccaccact tcgagctgcc ggatcgccgc cgggcgacgc
     2521 gtgtccgtgg ccaccagcag gggtgtgcca ccacgtttcg ccatcatccg cgc
//
LOCUS       DAOSPL010000612         2573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40152_length_2573_cov_3.227959, whole genome shotgun sequence.
ACCESSION   DAOSPL010000612 DAOSPL010000000
VERSION     DAOSPL010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40152_length_2573_cov_3.227959"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PLQ54_11420"
     CDS             <1..640
                     /locus_tag="PLQ54_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93915.1"
                     /translation="TPDMVYLLDRGARPAPDELWLGTLEADLGWLEILEPPETERDTA
                     AFLSGHSAACAPSPNADPFGLTKAINEKWPGSAYLPYAEFYAMWAEARRGRSHHGFRQ
                     AGLEELRQRAEAFRAAHPDFPLNHQLDTVVALYVYGLTPFPQRTLRVMGGTKPPTRES
                     VLRRYEAAGPLREAARASGDRGVMAEIEARIGWFELVCKSEIEAALAGREPE"
     gene            complement(1097..2161)
                     /locus_tag="PLQ54_11425"
     CDS             complement(1097..2161)
                     /locus_tag="PLQ54_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS93916.1"
                     /translation="MANTYRVAFIGTGRPWGHEGRTGFGMAYEHAHGYIASKRCELVA
                     AADIVEANARAFADHFGFATIYTDYKKMLKEIQPDIVSISTWPHLHCEMVVQTAKSKG
                     LKAIHCEKPMAPTWGEAQKMAAACEKAGVQLTFNHQRRFLEPFRMAREMAHDGTIGEL
                     VRLEGACADEIDWGTHWLDMFGFYNNETPAKWVMAQIHSVQERKIFGLDTTNQGLIEI
                     MYENGVRGLLFTGWDNDIGAMNRLMGTEGYIDLCDEQPHLRVRGKRSATLKGIKTKQG
                     LHGGVVEAVIDAVAALDEKREPELSAARALKATEVIFAAYESSRSRGRVDLPLKKKDS
                     AYLSMIASGDLKLAKPKPKE"
     gene            complement(2184..>2573)
                     /locus_tag="PLQ54_11430"
     CDS             complement(2184..>2573)
                     /locus_tag="PLQ54_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93917.1"
                     /translation="VVRVQGPFTDAMMEKTRTLACYEDAELVTEERDGKTVLREIRVP
                     RRYDWGDHVQNWIDCIRSRKQPTLNARLAYLVEVPIALSVMSFRQGRAIYFDPDTEQV
                     VDELPKPRKVREDIPSYLTGRKPGAKM"
BASE COUNT          376 a          824 c          902 g          471 t
ORIGIN      
        1 cacacccgac atggtgtacc tgctcgatcg tggagcgcgg cccgcgcccg acgaactctg
       61 gctcggcacg ctcgaggccg acctcggctg gctcgagatc ctcgagcccc cggagaccga
      121 gcgggacacg gcggcgtttc taagtgggca ctcagccgcg tgcgctccga gcccgaacgc
      181 tgatcccttc gggctgacca aggcgatcaa tgagaagtgg ccaggatcgg cctacctgcc
      241 ctatgccgag ttctacgcga tgtgggccga ggcgcgcagg ggccgcagtc accacgggtt
      301 caggcaggcc ggcctcgagg agctgcggca gcgcgccgag gccttccgtg cggcacatcc
      361 cgacttcccg ctgaaccacc aactcgacac ggtggtggcg ctctatgtgt acggcctcac
      421 cccgttcccg cagaggactc tgcgcgtgat gggcggcacg aagccgccta cgcgcgagag
      481 cgtgctccgg cggtacgagg ccgccgggcc actgcgcgag gccgctcgtg catcgggtga
      541 ccgaggggtg atggccgaga tcgaggcgcg catcggctgg ttcgagctgg tctgcaagag
      601 cgagatcgag gcggcccttg ccgggaggga gccggagtga cgatctgggc ggccatgctg
      661 ggcatcctgg cggccctggc gccgagtcag gcgcgcacgc gtaggtcgag cgtctcgctc
      721 gacaccgatg accgagccga ccgggacggt cggcctacgc gcgcggggtg gtggaacccg
      781 ggccggcggc agggcgatgg agagcgggcc ggatggcgcc cttcccgctt cgcgggcccc
      841 ttccatgcgg cccctcctcg gtttttggga ggtccttcgg aggggccgag cagaggcatt
      901 ggccctggcg tagggctcca ggcggctcat cggcctggtt gcggaggggc ccccaacgcg
      961 cccggggttg ccgtgccgac ctgggcccga tggcgccctt cccgcttcgc gggccccttc
     1021 catgcggccc ctcctcgttt gcggtgcggt cgttggaggg gccgcagcgg accggtctgg
     1081 actgattgcg ttgcgcctac tctttgggct tcggcttggc gagcttcagg tcgccagagg
     1141 cgatcatcga cagataggcc gagtccttct tcttcagcgg gaggtcgacg cggcctcggc
     1201 tacggctcga ttcgtaggcc gcgaagatca cctcggttgc cttcagcgcg cgggcggctg
     1261 agagctcagg ctcacgcttc tcatcgagag cagcgacagc gtcgatgacg gcctcgacga
     1321 caccgccgtg aagcccctgc ttggtcttga tgcctttgag ggtcgcgctg cgcttgcctc
     1381 ggacccgcag gtgtggctgc tcatcgcaca ggtcgatgta cccctcggtg cccatcaggc
     1441 ggttcatggc gccgatgtcg ttgtcccacc cagtgaagag caagccgcgg acgccgttct
     1501 cgtacatgat ctcgatgagc ccctggttgg tcgtgtcgag gccgaagatc ttgcgctcct
     1561 ggaccgagtg gatctgggcc atgacccact tggcaggcgt ctcgttgttg tagaagccga
     1621 acatgtcgag ccagtgggtg ccccagtcga tctcatcggc gcaagcgccc tcaagccgga
     1681 ccagttcgcc gatggtgcca tcgtgggcca tctcgcgcgc catgcggaag ggttcgagga
     1741 agcgtcgctg atggttgaag gtgagctgga cgccggcctt ctcgcaggcc gcagccatct
     1801 tctgcgcctc gccccaggtg ggtgccatgg gtttctcgca gtggatggcc ttgaggccct
     1861 tgctcttggc cgtctggacc accatctcac agtgcaggtg gggccaggtc gagatgctga
     1921 cgatgtccgg ctggatctct ttcagcatct ttttgtagtc ggtgtagatg gtggcgaagc
     1981 cgaagtggtc ggcaaacgcc cgggcgttcg cctcgacgat atcggccgcg gcgacgagct
     2041 cgcagcgctt cgaggcgatg tagccatgag cgtgctcata agccatgccg aacccggtgc
     2101 ggccctcatg cccccacggg cggccggtgc cgatgaacgc gacacgatag gtgttggcca
     2161 ttggttcccc tccggagtgg gatctacatc ttcgcgccgg gtttcctccc ggtcagatag
     2221 gacgggatgt cctcgcgaac cttgcgcggc ttggggagct cgtcgacgac ttgctcggtg
     2281 tcggggtcga agtagatggc cctgccctgg cggaaggaca tcaccgagag ggcgatgggc
     2341 acctcgacca ggtaggcaag ccgggcattg agggtcggct gctttcgcga gcggatgcag
     2401 tcgatccagt tttggacgtg atcgccccaa tcatagcgcc gcggaacgcg gatctcgcgc
     2461 aggacggtct tgccgtcgcg ctcctcggtg acgagctcgg cgtcctcgta gcaggccagt
     2521 gtcctggtct tctccatcat ggcgtcggtg aagggtcctt gcacccgaac gac
//
LOCUS       DAOSPL010000613         2572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40181_length_2572_cov_3.048868, whole genome shotgun sequence.
ACCESSION   DAOSPL010000613 DAOSPL010000000
VERSION     DAOSPL010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40181_length_2572_cov_3.048868"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..1380)
                     /locus_tag="PLQ54_11435"
     CDS             complement(250..1380)
                     /locus_tag="PLQ54_11435"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93918.1"
                     /translation="MAPGESVVIIGAGSIGRGLVADVVSAAGLRPVFVARQAKGKALR
                     QAGHYCIRLVGGGQSLVRTVSDYDVITTDDRRRLRAAIASCQFAATAVGSQNLPAIAP
                     LLVDGLADRAEPLNILLCENAPQGGEVLAQALAARGASRERFSCVRSSPEPMVRSLDR
                     SLDLVAERIRTTYVEAAHWLGPAPDIPGFDYWQDITPFYWRKLYTNNAGHALIAYMGA
                     YLGCTTVAEAVQRAPIRRHLEDLLHAAGAVLVHEFGLNPEATRDHLVQLQDERYPNAE
                     LGDTIARLARDPLRKLGPGERLVGLLRLLQAAHLSTLPVTRVIGAALRYRDPKDPSAA
                     ELARRVRRGGPGAVLESVCGIAAIEPCFAECIAAFEALECRR"
     gene            2329..>2572
                     /locus_tag="PLQ54_11440"
     CDS             2329..>2572
                     /locus_tag="PLQ54_11440"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOS93919.1"
                     /translation="MALVPMRVLLDEAAKGGYGVGAFNVNNMEQIQAIMMAAQKTQSP
                     VIVQASRGARKYSNDAYLRHLMLAAVELNPDIPIVMH"
BASE COUNT          438 a          867 c          864 g          403 t
ORIGIN      
        1 acggtctcgt agcggtcgaa tccgaagtag gcattcaggt ggacgtcggc cggcatgccc
       61 tctcgccgcc agcgcgacag cgtctcgccg aagaaccagt ggggcatcag aaaggggcgg
      121 tccggcttgc caaagtgcat ggtctcgagg aaccgttcgc gtgccgtcat gatggcagca
      181 ctccccaggg cggcccgcat tgcctggatt ggggccggcg ggaatagggt ttcccgccgc
      241 cgggcaccgc tacctgcggc actccagtgc ttcgaaagcc gcgatgcact cggcgaagca
      301 cggctcgatg gcggcaatcc cgcacaccga ctcgagtacc gcccccggtc cgccgcgtcg
      361 cacacgtctg gcgagctctg cggcggaggg gtctttcggg tcgcggtagc gcagtgcggc
      421 gccgatgaca cgcgtcacgg gcagggtgct cagatgggcg gcctgcagga gacgtagcag
      481 ccctaccagc cgctcgccgg ggcccagctt ccgaagcgga tcgcgtgcca gccgcgcgat
      541 cgtatccccg agctccgcgt tggggtaccg ctcgtcctgc aactggacca agtggtcccg
      601 agtcgcctcg gggttgagtc cgaactcatg cacgagaacc gcaccggccg catggagcag
      661 atcctcgaga tgacgccgta tcggcgcccg ctggacggct tcggctaccg tggtgcagcc
      721 caggtaggcc cccatgtagg cgatgagcgc gtggcccgca ttgttggtgt agagcttccg
      781 ccagtagaag ggagtgatgt cctgccagta gtcgaaaccg gggatatccg gcgccggacc
      841 caaccaatgg gcagcctcga cataggtggt tcggatacgc tccgccacca ggtccaggct
      901 cctatcgagg gagcgcacca tcggctcggg cgatgaccgg acgcacgaga aacgttcgcg
      961 cgaggcaccc cgcgccgcca gggcctgcgc gagcacctcg ccgccttgcg gggcattctc
     1021 acatagcaga atgttcagcg gctccgcccg atccgccagt ccgtcgacca ataggggtgc
     1081 gatggccggc aggttctgcg agccaaccgc cgttgcggcg aactggcacg aagcgatggc
     1141 ggcgcggagg cgcctccggt cgtcggtggt gatgacgtcg tagtctgata cggtgcgcac
     1201 caagctctgg ccgccaccaa cgaggcgaat gcagtaatgg ccggcctgac gaagtgcctt
     1261 ccccttagcc tgccgagcca cgaacacggg acggaggcct gccgcagaca cgacgtcggc
     1321 caccagcccc cggccgatgc taccggcccc gatgatgacc acactctcgc ccggcgccac
     1381 acccagcctc cacccgtgcc caacggccac aagagtaaga acgttgaagg ttcgccgacg
     1441 tttcgtccga gccctcccag ggctggccca tccaccaatg acctgaccgt cgctagattg
     1501 gtggcacggg acgggtctgt ctgctagatg catggccggg ttgctgagag acgcacagga
     1561 tgtggcggca acgtggtggg gcattggacc gccggtacgc tcgcttcggg cgctgaaggg
     1621 ctacggatcc tgagggagga gctgctgtgg ggttcccgag cccatccata ccgcaccgat
     1681 cggtgggcct tggctgacat cgcccagctc accaggaacc ggtccggtgt gcggcggtcc
     1741 gtgactgggg agtggcgcat accgtagggc atgggcgttg gctgccggaa gcccgcgcag
     1801 cggtcagtgc ggagtggctg acggcggagc acctcccgcc gcacgcgccc ggacccaacc
     1861 tggtggcgca ggtgtgggcg gactcgcggg ggaggaaact ggctccgcac gcctctgata
     1921 gcccgggcga gctttccgga cattccgccc gcgatggacg ccgcgtcggc tgggtcaagc
     1981 gccgctcagg cttcatccag cattccagac tcctggacct cggcatcacc agacaagagt
     2041 ggcaagatca tcggcagggc catgcatatc ccccacgagg ccgtgggcct cctcagacac
     2101 gagtcggctc gggccgtatg cagtcccacc agccgctcgc cttggcattc gggcgcgttt
     2161 gggcgataat gtggcccgcg tcggcgactc gcttgcccct gccggcgcga cttcggaggg
     2221 ctcggcgcca cactcggagg gtgctgagcg aatctgacac tcccgtgaac gccggcccgg
     2281 gctcgtgcgc ccgccggtga ccgtcacaca gaaagggatc gtgctgctat ggctcttgtg
     2341 ccgatgcgtg tcctcttgga cgaggccgcc aagggcggct atggcgtggg cgccttcaac
     2401 gtgaacaaca tggagcagat tcaggccatc atgatggccg cccagaagac ccagtcgccg
     2461 gtcatcgtcc aggccagccg gggagcccgc aagtactcga acgacgccta cctccggcat
     2521 ctcatgctcg ccgccgttga gctgaacccc gacatcccca tcgtcatgca cc
//
LOCUS       DAOSPL010000614         2567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40285_length_2567_cov_3.051354, whole genome shotgun sequence.
ACCESSION   DAOSPL010000614 DAOSPL010000000
VERSION     DAOSPL010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40285_length_2567_cov_3.051354"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="PLQ54_11445"
     CDS             complement(<1..1050)
                     /locus_tag="PLQ54_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015495545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93920.1"
                     /translation="MSGPAQLTSFVLALSVVPGSAQTLLPWQEWVYEPSTTEVRDLGT
                     LTNLPWQNSGGKEGRLGVSESPGPWGGRFLDFRVEIDHLDAGAYPMGWPSFELRPDPP
                     MDLSGYDALQYWVRCEGTTAKPFTLRFILWTDGAGRVNQPIAGLRRGEWVQVTQRLDG
                     IPHMDRVDRIHFFLCESDYAHGDQLRFQVGGFRLCDLDRSASRLPPGQAAAGLWVGER
                     ADTGDQAVIIDQGTARIPGLLAVETGSAVAFRASDELAVRYHSVFSGEDRTASLRLGQ
                     EVAPGQMARVTTSLPTAGLAPGYYLVVADIRRAVFDRVVGMSPAFYERLLTGEILSRI
                     TTDTTLILSVIGSSVS"
     gene            complement(1393..>2567)
                     /locus_tag="PLQ54_11450"
     CDS             complement(1393..>2567)
                     /locus_tag="PLQ54_11450"
                     /inference="COORDINATES: protein motif:HMM:NF028151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HOS93921.1"
                     /translation="SPELLAQTAGDALGPWSFRPASGPLARVSEGHLAQHLAREDLYW
                     LGPHVGASYSQAPPAPNIIDQALVPTSRGRNLNAIEAETLPVSGPIVQLIENGSAVGM
                     FTNGDLTAEIDFGPGGPLLIGGRLGGTPAGGTWPQCRISVDGAAAVQISTAGQAYDDY
                     VALMRVPGGTHRVTLSFVNDGSSGTEDRNLFVDKVLWGACELGDASMEAITEPAGVAV
                     IPVGAGRVVLDCVRWDAPGSNRVRAQRYLCGLLTALDADMEARADAESVQLSGLTVDP
                     GVAYGGTDGGTLHFGSSGAASGEIEVVRAGRYALEITGWGTPAAGEFPIVEILLDDRL
                     LGRVAVASTRLSSHTLGEAELPGGIHRLVVRFVNDLYSPPEDRNVWLDALKAYPAR"
BASE COUNT          419 a          891 c          894 g          363 t
ORIGIN      
        1 cgagacggac gagccgatga ccgacaggat cagcgtggtg tcggtggtga tgcgtgaaag
       61 gatctcgccg gtcagaaggc gttcgtagaa ggccgggctc atgcccacca cccggtcaaa
      121 gaccgcgcgg cggatgtcgg ccaccaccag atagtagccg ggagcgagac ccgccgtcgg
      181 cagtgaggtg gtgactcggg ccatctgccc gggcgcgacc tcctggccga gccggagtga
      241 ggccgtccgg tcctcgcccg agaacacgct gtggtaacgc acagccagct cgtccgacgc
      301 gcgaaaagcg accgcgctac cggtctcgac tgcgagcagg ccgggaatcc tggcggtccc
      361 ctggtcgatg atcacggcct ggtcgcccgt gtcggcgcgc tcgccgaccc aaagccccgc
      421 ggcggcctgc ccgggcggaa ggcgtgaggc cgagcggtcg aggtcgcaca ggcggaagcc
      481 gccgacctgg aagcggagct ggtcgccgtg ggcatagtcg ctctcgcaca ggaagaagtg
      541 gattcggtcg acccggtcca tgtgggggat gccgtccaac ctctgcgtca cctgaaccca
      601 ctcgcctcgg cgcagccccg cgatgggctg attgactcgc cccgcgccat ccgtccagag
      661 gatgaaccgc agcgtgaagg gcttggccgt agtcccctcg cagcgcaccc agtactgcag
      721 ggcatcgtag cccgataggt ccatcggcgg gtcgggccgg agctcgaatg agggccaccc
      781 catggggtat gcgccggcat cgaggtgatc gatctccact cggaagtcga ggaaccgccc
      841 gccccaaggc cccggactct ctgagacccc aagccgtcct tccttgccgc ccgagttctg
      901 ccacgggaga ttggtgaggg tgcccagatc gcgcacctcg gtggtcgagg gctcgtagac
      961 ccactcctgc caggggagca gcgtctgggc cgaccccggg acgaccgaca gagcaagtac
     1021 gaacgaggtc agttgcgccg ggccgctcat cacgcatcgc ctccaagtgc acgggtagcg
     1081 cagggcattg tgcgcgccgg tcgagcgcaa gacctccctg ggtccggccg acgagcagac
     1141 accccggggg ttccgcccca gaacccggcc cgaggagcca tcggctcccg ccgcccttcg
     1201 tcacgccgcg tggacgcggc ggctcagggt cggcgggttg ggggctggcg cggccccccg
     1261 gcgcggagac cgcgagctac ggcaaggggt tgtcgcatca cgcccgccgc tcgcgcagcg
     1321 gcctaccacg tgtcgctcct tcggcggctt gaggcaggct gatgtcagcc ccatagggtc
     1381 ggtactggcc gactacctgg ccgggtacgc cttcagagca tcgagccaca cgttgcggtc
     1441 ctcgggcggg ctgtagaggt cattcacgaa ccggacgact agcctgtgga tccccccggg
     1501 cagctcggcc tcgcccagtg tgtggctgga caatctcgtg ctcgccacag ccaccctgcc
     1561 caggagccga tcgtcgagca ggatctcgac gatggggaac tcgccggccg cgggagtccc
     1621 ccagccggtg atctccagcg cgtagcggcc ggcccgcacg acctcgatct cccccgacgc
     1681 cgctcccgag gagccaaagt gcagcgtccc gccatcggtg ccgccatagg ccacgccggg
     1741 gtcgacggtc agtccgctaa gctggaccga ttccgcgtcg gcccgcgcct ccatatccgc
     1801 atcgagagcg gtaagaagcc cgcagaggta ccgctgcgca cgaacgcggt tgctgccggg
     1861 cgcatcccag cggacacagt cgagcacaac gcgtcccgca ccaacgggta tgacggcgac
     1921 ccctgctggc tcggtgatcg cttccattga cgcgtcgccg agctcgcagg ctccccacag
     1981 gaccttgtcg acgaaaaggt tgcggtcctc ggtgccacta gacccgtcgt tgacgaagct
     2041 cagcgtcacc cggtgggtgc caccgggcac gcgcatgagg gcgacgtagt cgtcataggc
     2101 ttggccggca gtcgagatct ggacggcggc ggccccgtcg acggagatcc ggcactgggg
     2161 ccaggtgccg cccgccggcg tgccacccaa tcggccgccg atgaggagcg gtccgcccgg
     2221 gccgaagtcg atctcggcag tgaggtcgcc gttggtgaac atgcccacgg cgctgccatt
     2281 ctcgataagt tggacgatgg ggccagacac gggtagcgtc tcggcctcga tggcgttcag
     2341 gttccgcccg cgagaggtcg gcaccagggc ctggtcgatg atgttcggcg ccggtggggc
     2401 ctggctgtag ctcgcaccga catgcgggcc cagccagtag aggtcctccc gagccaggtg
     2461 ctgagccaga tgaccctcgg acacccgggc cagtgggcca gaggccggcc ggaaagacca
     2521 cggccccagg gcatcgccgg cggtctgggc caacagctcg ggcgagg
//
LOCUS       DAOSPL010000615         2566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40304_length_2566_cov_3.003584, whole genome shotgun sequence.
ACCESSION   DAOSPL010000615 DAOSPL010000000
VERSION     DAOSPL010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40304_length_2566_cov_3.003584"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PLQ54_11455"
     CDS             complement(<1..623)
                     /locus_tag="PLQ54_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93922.1"
                     /translation="MDDPLFTDFAAIPASLAQRHQWCLWRRVMRGGRPTKVPFQPSGE
                     PARSNDPATWCSFDDALFALMSGEFAGLGIMLGHGLFGIDLDAVRDPDTGALTPQAQD
                     IIQRLSSYTEVSPSGKGVHILFEGDRLPDGRRRNGPVELYGPGSPRFLTVTCRPVPGT
                     PRDIAHRQAEMEAVHAEYVAGAASPTTTHPVPAGVLARQDDGAGQEPR"
     gene            803..2017
                     /locus_tag="PLQ54_11460"
     CDS             803..2017
                     /locus_tag="PLQ54_11460"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOS93923.1"
                     /translation="MGRLVLGIASTLVCLLVAELATRWLLPVPLHTTDTFLHLGDARL
                     RRDESAPVWLRAGVRTRHQEEDFSVTVAVNSKGLRDREMAYAKPPGVRRLLSVGDSFA
                     FGYGVELDEAFCKVAAAELGPPWEAINAGVPSWGTGDELDFLLTEGFRYEPDVVVLTC
                     FFENDLRDNWNRQRYTVGADGRARRVRPLEQDLRERTSFDGVSVRNPLLKTICERGAP
                     ADATEPAAPAPPRVSWWIAHSNLARMVRRAISRTEYRTREQAMQEAAHRDEAERRALG
                     AALLTEIARQCRAHDTPLVILLMPSKKELAWNDGPGFRAAYDDVVAGAEQWGATIVDL
                     APPLRELGIQTAYFGNDPHINAAGHRAVGHVLARAVRSAWRGRGGPAHPGASGVPPQL
                     GPWVDRVLGRRR"
     gene            complement(2000..>2566)
                     /locus_tag="PLQ54_11465"
     CDS             complement(2000..>2566)
                     /locus_tag="PLQ54_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS93924.1"
                     /translation="LYYVLNVLFGARKQVFITFGRWVLVTVFFQTPTAFAQIWIVASA
                     IGIWFNPLLGRMVDRLGERTVLIIDSFVMMLVCFIYGGAEHLGLSRAAATYLVMGCFM
                     LDQLFFATGMARETYLAKIARSKDDLTATLSMGVTINHLIAATIPTLGGWAWHRYGYE
                     YVFAGAGVVAVMLLVFSAMIPRRSPAAT"
BASE COUNT          391 a          822 c          956 g          397 t
ORIGIN      
        1 cggggctcct gccccgcgcc gtcgtcctgg cgggcgagga cgcccgccgg cacggggtga
       61 gtggtggttg gcgatgccgc gcccgccaca tactccgcgt gcacggcctc catctcggcc
      121 tgacggtgcg cgatgtcccg cggcgtgcca ggcacaggcc gacaggtgac ggtgaggaac
      181 cgcgggctgc cggggccgta gagctcgacg ggtccgttcc gccggcggcc gtcgggcagc
      241 cgatcgccct cgaagaggat gtggacgccc ttgcccgacg ggctgacctc ggtatacgag
      301 gatagacgtt ggatgatgtc ttgggcctgc ggcgtcaggg ccccggtgtc gggatcgcgc
      361 acggcgtcga ggtcgatgcc gaacaggccg tggccgagca tgatgcccag gccggcgaac
      421 tcgccggaca tgagggcgaa gagggcgtca tcgaaggagc accaggtggc gggatcgttg
      481 gagcgcgccg gttcgccgga gggctggaag ggcaccttgg tgggccgtcc accgcgcatg
      541 acgcgtcgcc agaggcacca ctggtggcgc tgggcgaggg aggcgggtat ggcggcgaag
      601 tcggtgaaga gggggtcgtc catggcggga tcgctcgctt gcggggaggc gttccggcgc
      661 gctatgaccc tattatacat gatttcagac gttttgtcaa gcatgtgatg cgatcttttg
      721 tgtgcttgcg atgaggcgat ccggtagttg ccccgaccgt cggtgtacaa tcggccccat
      781 gatgccgagg ctccgacagg ccatgggcag gctcgtgctg ggcatcgcca gcacgctggt
      841 ctgcctgctg gtggccgagc tggcgacccg gtggctcctg ccggttcccc tacacaccac
      901 cgacacgttc ctgcacctgg gtgacgcccg cctccggcgg gatgagtcgg cgcccgtgtg
      961 gttgcgggcg ggcgttcgca cgcggcacca ggaggaggac ttctcggtca ccgtcgcggt
     1021 gaactccaag ggtttgcgtg atcgcgagat ggcctacgcg aagcccccgg gggtgcgtcg
     1081 gctgctctcg gtgggcgact ccttcgcgtt cggctacggc gtcgagctcg atgaggcgtt
     1141 ctgcaaggtg gcggcggccg agctgggccc gccgtgggag gccatcaatg ccggcgtgcc
     1201 ctcgtggggc accggtgacg agctggactt tctgctgacc gagggcttcc gctacgaacc
     1261 cgacgtggtg gtgctcacct gcttcttcga gaacgacctg cgggacaact ggaaccggca
     1321 gcgctacacg gtgggggccg atggccgcgc ccggcgggtg cggcccctcg agcaggactt
     1381 gcgcgagcgc acgagcttcg acggcgtgtc ggttcggaac ccgttgctga agaccatctg
     1441 cgagcgcggc gcgcccgccg acgcgacgga gccggcggct cccgcccccc ctcgcgtgag
     1501 ctggtggatc gcccactcga acctggcgcg catggtgcgt cgggcgatct cacgcaccga
     1561 gtaccgcacg cgcgagcagg ccatgcagga ggcggcccat cgcgacgagg ccgagcgccg
     1621 ggccctcggt gcggccttgc tgaccgagat cgcgcggcag tgccgcgcgc acgacacgcc
     1681 gctcgtgatc ctgctgatgc catcgaagaa ggagctggcg tggaacgacg gcccgggctt
     1741 ccgggcggcc tatgacgacg tggttgccgg ggccgagcag tggggagcca cgatcgtcga
     1801 tctcgccccg ccgcttcgcg aactcggaat ccagacggcc tatttcggga acgatcccca
     1861 catcaacgcg gcgggccatc gggccgtggg acacgtgctg gcccgggccg tgcgctcggc
     1921 gtggcggggg cgcggtgggc cggcccaccc gggcgcgtcg ggggtcccgc cgcaacttgg
     1981 cccatgggtt gatcgagtcc taggtcgccg ccggtgacct ccgggggatc atcgccgaga
     2041 acaccagcag catcacggcc acgacgccgg cgccggcgaa cacgtactcg tagccatacc
     2101 tgtgccacgc ccagccgccc agggtgggaa tggtggccgc gatgaggtgg ttgatggtga
     2161 cccccatcga gagggtcgcc gtcagatcat ccttgctgcg ggcgatcttc gccaggtacg
     2221 tctcccgcgc catgcccgtg gcgaagaaga gctggtcgag catgaagcag cccatgacca
     2281 ggtacgtggc ggccgctcgg ctgaggccca ggtgctcggc gccgccgtag atgaagcaca
     2341 cgagcatcat cacgaaggag tcgatgatga gcacggtccg ctcgcccagg cgatcgacca
     2401 tgcggccgag cagcgggttg aaccagatgc cgatggctga ggccacgatc cagatctggg
     2461 cgaaggcggt gggtgtctgg aagaacaccg tgacgagcac ccagcgtccg aaggtgatga
     2521 agacctgctt gcgcgccccg aatagcacgt tgagcacgta gtagag
//
LOCUS       DAOSPL010000616         2563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40355_length_2563_cov_3.654306, whole genome shotgun sequence.
ACCESSION   DAOSPL010000616 DAOSPL010000000
VERSION     DAOSPL010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40355_length_2563_cov_3.654306"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PLQ54_11470"
     CDS             complement(<1..625)
                     /locus_tag="PLQ54_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HOS93925.1"
                     /translation="MYQLSDGVFLTENACIVLQKRYLRKDARGEPCETPVQMFRRVAE
                     NIASADRRYGCSEDHVEALSRAFFGLMTSLEFLPNSPTLMNAGRELQQLAACFVLPVE
                     DSIESIFDAIKHTALIHKSGGGTGFDFSHLRPKDDIVRSTHGVSSGPVSFMAVFNAAT
                     EAIKQGGTRRGANMAMLRVDHPDILEFIGCKTSNQALANFNISVAVTH"
     gene            complement(613..>2563)
                     /locus_tag="PLQ54_11475"
     CDS             complement(613..>2563)
                     /locus_tag="PLQ54_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93926.1"
                     /translation="GYGSSPMVEYDGAGDYFLDRVSPGVWRLEVFPDAVWVDDPFAGR
                     STAGDVSQLDRASRVFWRPRSMRVRLPDLGKVFTVTRIAGGRPLGSEDVEASEAEPAT
                     ERIPLVNGRFACLPGVYVLRDDSASEPSPPISTTFVAPPPDQGGPTVWHQPRRAIAAG
                     EPLTIRATVASEGDPAVHVHYRLPGTDRFEARPMDPVQPYVYEVMLAPELLGKGDLDY
                     WISVREGGEVLNWPGAWIGAGDAAGPAVPEPVLLWNAGEQAPGTGPDLVEAEGRICSS
                     RVVRRSDGQGRALQIDVPAYGDKGVVVLRHPVAAVNPETGDDAVEIVARSERNAQFLE
                     LALVEADGTPWGTNVALTSEWRTITVPLSRLWRLRGFPARTVHDRVQIGNIRQVEVLS
                     GAWLHPETKGEPHSFQVDTIRLVSCPRTYRVAVGPDGPLVLFRASGEGRQPKVEFQQM
                     DGRPGGMRLTAGMSADSVAVRLIADHGFGASGYGAMANAVELEGYRLGDYGVLRVRCR
                     GAATTNRLEVALVESDGRGWGVNVPVTEDWQDVRIPLGDLKLLWGYEPRGPGDRVRLE
                     SLERVQCCFGAWLFPDTPDGSHWVEVEEIALEPGPPPSTPDATAAPAEDEAEGSNAAP
                     DEPEAPDEPGVGPAASMRETNVCTS"
BASE COUNT          398 a          951 c          824 g          390 t
ORIGIN      
        1 cgtgggtcac ggccaccgag atgttgaagt tggccaacgc ctggttcgag gtcttgcagc
       61 cgatgaactc gaggatgtcg ggatgatcca cccgaagcat cgccatgttg gcaccgcggc
      121 gcgtgccgcc ctgcttgatg gcttccgtcg cggcgttgaa gaccgccatg aacgataccg
      181 gtccgctgga caccccgtgg gtggaccgga cgatgtcatc cttgggccgc aggtgggaga
      241 aatcgaagcc ggttccgccg ccgctcttgt ggatcagggc cgtgtgtttg atggcatcga
      301 agatactctc gatgctgtcc tccacgggca ggacgaagca ggcggcgagt tgctgcagct
      361 cccggcccgc gttcatcagc gtcggcgagt tgggcaggaa ctcgagcgag gtcatcagcc
      421 cgaagaaggc ccggctcagt gcctcgacgt ggtcctccga gcaaccatag cggcgatcgg
      481 cgctggcgat gttctcggcc acgcggcgga acatctgtac cggggtctca cacggctctc
      541 cccgggcgtc cttccgcaag tagcgcttct ggagcacgat gcaggcattc tcggtcagga
      601 acacaccgtc gctcagctgg tacatacgtt ggtctccctc atcgacgcgg ccggccccac
      661 gccgggctcg tcgggcgctt cgggctcgtc gggcgccgca ttggacccct cggcctcgtc
      721 ctcggctggg gccgctgtcg catccggcgt ggacggcggt ggtcccggct ccagcgcgat
      781 ctcctccacc tccacccagt gtgatccatc aggcgtgtcc gggaacagcc atgcgccgaa
      841 gcagcactgc acacgctcca ggctctcgag ccgcacccgg tcgcccggcc ccctgggctc
      901 atacccccac aggagcttga ggtcacccag ggggatccgc acgtcctgcc agtcctcggt
      961 cacggggaca ttcacgcccc agccccgccc gtcggactcg accagagcca cctcgagccg
     1021 gttcgtcgtc gccgccccgc ggcaccgcac gcggagcacg ccgtagtcgc cgagacggta
     1081 gccctcgagt tcgacggcat tggccatcgc cccatagccc gatgcgccga acccgtggtc
     1141 cgcaatcagg cgcacggcca cggagtcggc cgacatgccc gcggtcaggc gcataccgcc
     1201 ggggcggccg tccatctgct ggaactcgac cttgggctgc cgcccctcgc cgctggcgcg
     1261 gaacagcacg agcggtccgt caggccccac cgccacgcga taagtccgcg ggcagctcac
     1321 caggcggatc gtgtcgacct ggaagctgtg cggctcgccc ttggtctccg ggtggagcca
     1381 cgcgccggat agcacctcta cctgccggat gttcccgatc tgcacccggt cgtgcaccgt
     1441 gcgcgcgggg aaaccacgga gtcgccacag ccgcgacagc gggaccgtga tggttcgcca
     1501 ctcactcgtc agagccacat tggtgcccca cggcgtcccg tcggcctcaa cgagagccag
     1561 ctcgaggaac tgcgcgttcc gctcgcttcg cgccacgatc tcgaccgcgt cgtcacccgt
     1621 ctccgggttg acggctgcca cggggtggcg caggacaacc acgcccttgt cgccgtaagc
     1681 cggaacgtca atctggagcg cccgtccctg cccgtccgac cggcgcacca cccggctcga
     1741 gcagatccgc ccctccgctt cgaccagatc cggcccggtc cctggcgcct gctcgcccgc
     1801 gttccagagc aacacgggct caggtacggc gggtcccgcc gcatctccgg caccgatcca
     1861 ggccccgggc cagttgagca cctcgccccc ctcgcggacc gagatccagt agtcgaggtc
     1921 ccccttgccg agcagctcgg gcgcgagcat gacctcgtac acatagggct ggaccggatc
     1981 catcggccgg gcctcaaacc gatcggtgcc gggtagccgg tagtgaacat gcaccgccgg
     2041 atcgccctcg ctggccacag tggcgcgtat cgtcaggggc tcgccggccg cgatggcccg
     2101 acgaggctga tgccacacag tcggcccgcc ctgatcgggc ggaggcgcga cgaacgtcgt
     2161 gctgatcggc ggggacggct cggaagcgct gtcgtcgcgg aggacgtaca cccctggcag
     2221 acatgcgaac cgcccgttca cgaggggaat gcgctccgtc gccggctccg cctcactggc
     2281 ttcgacgtcc tcactgccca gtggccgccc ccccgcgatg cgcgtgactg tgaacacctt
     2341 ccccagatcc ggcagccgaa ccctcatgct gcgcgggcgc cagaagaccc ggctcgcccg
     2401 gtcgagctga gagacgtcgc ccgcggtgct gcggccggcg aagggatcgt ccacccagac
     2461 agcatccggg aacacctcga gccgccagac cccgggcgat acccggtcga ggaagtagtc
     2521 gcccgcaccg tcgtactcga ccatcggcga cgatccgtag ccc
//
LOCUS       DAOSPL010000617         2562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40374_length_2562_cov_3.343039, whole genome shotgun sequence.
ACCESSION   DAOSPL010000617 DAOSPL010000000
VERSION     DAOSPL010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40374_length_2562_cov_3.343039"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PLQ54_11480"
     CDS             complement(<1..478)
                     /locus_tag="PLQ54_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS93927.1"
                     /translation="MRKRRRGFTLIELLVVIAIIAILAAILFPVFAKARAKARQASCQ
                     SNLKQIGLAWNMYAQDYDGVLTYLWPTPSWSSYWIRVQPYMKNIQLLQCPANVDTACA
                     CGYGGGTGDPNYIPASYMYNMWLTVSNGTYDNLDGKKLDSIQAPATTIMQFDGRRSI"
     gene            872..>2562
                     /gene="uvrB"
                     /locus_tag="PLQ54_11485"
     CDS             872..>2562
                     /gene="uvrB"
                     /locus_tag="PLQ54_11485"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOS93928.1"
                     /translation="MTVPGAGPPRVHTAGRFVLECEHRPTGDQPRAIDFLAEGVLAGA
                     PHQTLLGVTGSGKTFTIANVIERVQRPTLVIAHNKTLAAQLCAELRSYFPHNAVGYFV
                     SFYDYYQPEAYNPRTDTYIEKDSQINDEIDRLRHAATQAVSQRRDVVVVASVSCIFGL
                     GAPDEYRDIVLSLGLGESIERDDMLRQLVAMQFTRSTQSLSRGRFRARGDCVEVYPAD
                     RDVVYRVELFGDEVERISEIDPLTGNVLAQPSALEVYPATHWVASEARLLTAIENIEA
                     ELEERLEFFRKHGKLLEAQRLEQRTRYDLEMLREVGYCSGIENYSRHFDGRSAGQPPY
                     TLLDYFPEDYLLVIDESHQTIPQLRGMWAGDHSRKQNLVDYGFRLPCAFDNRPLTFDE
                     FQSRARQVIYTSATPSAYELTNSRDRVVEQVIRPTGLVDPSIDVRPTRGQIDDLIGEI
                     RLRVAQGQRCLVTTLTKRMAEDLTDYLADLDIRVRYLHSDVKTLDRGEILRDLRLGDF
                     DVLVGINLLREGLDLPEVSLVAILDADSEGFLRSAPSLIQIIGRAARNVDGHVLM"
BASE COUNT          442 a          768 c          870 g          482 t
ORIGIN      
        1 ggatgctgcg acgaccgtcg aactgcatga tggtcgttgc gggggcctgg atcgagtcga
       61 gtttcttgcc gtccaggttg tcgtaggttc cattgctgac ggtcagccac atgttgtaca
      121 tgtagctggc ggggatgtag ttcggatccc cggtgccgcc cccgtagccg cacgcgcagg
      181 cggtgtccac gttggcgggg cactgcagga gttggatgtt cttcatgtag ggctgtacgc
      241 ggatccagta gctcgaccac gacggggtag gccagaggta ggtcagcacg ccatcgtagt
      301 cctgcgcata catgttccag gccagcccga tctgcttgag gttggactgg caggatgcct
      361 ggcgcgcttt ggccctcgct ttggcgaaga ccgggaacaa gatggcagcc agaatcgcga
      421 tgatcgcaat cacgacgagg agctcgatga gggtaaaccc tctccgacgc ttacgcatgt
      481 gaagctctcc ttccgacggc aaacctagat gataaagtgc gcaatacgag cgccatttgc
      541 gaagtccact atcattacca gcccccaatc cttatatagc tactagtact aatggacatt
      601 atctcccaca caccggccgt ggtggtgatt cgactcggcc cggtctgggt cctcctgccg
      661 acggggactc gaggtgcaga ggctggtgca ttcgctcgca tgcgggtggg gccctgcttg
      721 gttcagggcc gtctgtgcct gagagagctc acggcccggc ggaagaacgg gaggacgtat
      781 cagcggtgcg cccaacctcg gctgtgcggc cgaatggggt atgatcaatg gttacccgcc
      841 cggcgtcggg catagcgatg gaggcgactc gatgacagtg cccggagcag gcccgccccg
      901 cgttcacacg gcgggccggt tcgttctcga gtgcgagcac cggcccacag gcgaccagcc
      961 ccgggccatc gacttcctgg ccgaaggcgt cctggcgggc gccccccacc agacgctgtt
     1021 gggcgtgacg ggctccggca agacgtttac gatcgccaac gtcatcgaga gggtccagcg
     1081 tccgaccctg gtcatcgccc acaacaagac gctggcggcc cagctttgcg cggaactgcg
     1141 gtcgtacttc ccccacaacg ccgtgggtta ctttgtgagc ttctacgact actaccagcc
     1201 cgaggcgtac aacccgcgga cggacaccta catcgagaag gactcgcaga tcaacgacga
     1261 gatcgatcgg ctccgccacg cggcgaccca ggcggtatcg cagcggcggg atgtggtcgt
     1321 ggttgccagc gtgtcgtgca tcttcggtct gggggctccg gacgagtatc gcgacattgt
     1381 cttgtcgctg gggcttgggg aaagcatcga gcgcgatgac atgctccggc agctggtggc
     1441 gatgcagttc acgcggtcga cccagtcgct ctctcgaggc cggttccgcg cccggggtga
     1501 ctgcgtggag gtttacccgg ccgaccgcga tgtggtgtat cgcgtggagc ttttcgggga
     1561 cgaggtggag cggatcagcg agatcgatcc gctgacgggc aacgtgctcg cccagccgtc
     1621 ggcgctggag gtctatcccg ccacccactg ggtggcctcc gaggcgcggc tgctgacggc
     1681 catcgagaac atcgaggccg agctcgagga gcgcctggag ttcttccgga agcacggcaa
     1741 gctgctcgag gcgcagcggc tggagcagcg cactcggtat gacctcgaga tgcttcgcga
     1801 ggtgggttac tgcagcggga tcgagaacta ctcgcgccac ttcgacgggc gcagcgccgg
     1861 gcagcctccg tacacgctgc tggactactt ccccgaggac tacttgctgg tcatcgacga
     1921 gtcgcatcag acgattccgc agcttcgggg gatgtgggcg ggggatcatt cgcgcaagca
     1981 gaacctggtg gactacggtt tccggctgcc ttgcgcgttt gacaatcgcc cgttgacctt
     2041 cgacgagttc cagagccgcg cgcggcaggt gatctacacg tcggcgacgc cgagtgcgta
     2101 tgagctgacc aacagccgtg atcgcgtggt ggagcaggtg atccggccga cagggctcgt
     2161 cgatccctcg atcgatgtgc gcccgacacg gggccagatt gacgacctga tcggcgagat
     2221 tcgcctccgt gtggcgcagg ggcagcggtg tctggtgacg acgctgacca agcgcatggc
     2281 ggaagacctg acggactacc tggcggacct ggacattcgc gttcggtacc tgcactcgga
     2341 tgtgaagaca ctggaccggg gcgagatcct gcgcgacttg cggctgggcg acttcgatgt
     2401 gctcgtgggg atcaacttac tgcgcgaggg gctcgacctg cccgaggtgt ctttggtggc
     2461 gatcctggat gccgactccg agggtttcct gcggtcggcg ccatcgctga tccagatcat
     2521 tgggcgcgcg gcccggaacg tcgatgggca cgtgctcatg ta
//
LOCUS       DAOSPL010000618         2560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40422_length_2560_cov_2.936128, whole genome shotgun sequence.
ACCESSION   DAOSPL010000618 DAOSPL010000000
VERSION     DAOSPL010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40422_length_2560_cov_2.936128"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PLQ54_11490"
     CDS             <1..362
                     /locus_tag="PLQ54_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93929.1"
                     /translation="KGDLVDRGTVPPASALVFDATGYWQGKPDLGPVELGSTVGVARP
                     AAPQLDRFAFASPRAGQATLKLHSASGRLLASLPLTVQEGRNRMEIPAGIHPGASFAT
                     VDLDGIRLHRGAVARVE"
     gene            complement(372..1394)
                     /locus_tag="PLQ54_11495"
     CDS             complement(372..1394)
                     /locus_tag="PLQ54_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93930.1"
                     /translation="MSAGRKLYAGIQAELDELTSRQILTPGQSAPLKERYPTTPWDIL
                     PLVRIFTVLGVLTAMTGTVILVKDMLDFWLLSELGLLGTVVGFIALGRWLKLSKGMPL
                     LGESLELAGGMALQGLTIVLAIHYSTGSKNWPSLVGVDAALLVGLAYALANPLVLWYA
                     CANLFFFLGAETGYLSDWGAYYLGMTYPVRYLAIAVVTLGLAYVHAVGLRGRWVRFSR
                     VYAHYGLLIANMAMWFMSLFGYYEDIDQIWSGRGSERLVFSILWFGLSVLSMVVSSKT
                     GWKLLRKYAFTFLVINLYTFYFQFVVANSGEAWFLHLLLTGGSLVGLGIFLERRRKHG
                     KIAEPE"
     gene            complement(1391..2380)
                     /locus_tag="PLQ54_11500"
     CDS             complement(1391..2380)
                     /locus_tag="PLQ54_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93931.1"
                     /translation="MTTRAVKQALADDISDWFADGVIEQKTLETLRERYQVEAFGVAG
                     FVRYLGIAGGLIAFFGIMGMIAAMSNSVHFGAVVTAAVGCGLVWWGIRLAGDIRERYA
                     TSSKIVLTLGAVLWTSGMMLFAQAVGAPEDVLIMIAGTSIPVMAVLAYKYHNSMLLLL
                     SVASLFHWVGAWNGMLGRSTYGFHVQDPRAMSVFALLAFGVGVYHELRLADRTGRFYK
                     VWESLGLLYLNMSLLILSIWRQTSEGELVWSLVLAAATVGQILLGARLHNGMIRGFGV
                     VFFAINVFTRYHEAFWDRLELGSYLLLGGLVLLALGGCMEGIVRAVRMKGAHS"
     gene            complement(2377..>2560)
                     /locus_tag="PLQ54_11505"
     CDS             complement(2377..>2560)
                     /locus_tag="PLQ54_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93932.1"
                     /translation="DDIGRLTFLRQVMSETLRDNTHVMTRVRNNTRDQAMRASLPGAA
                     VEAIVNTMITQARGAT"
BASE COUNT          516 a          852 c          768 g          424 t
ORIGIN      
        1 ccaagggcga tctggtggac cgcggcacgg tgcctcccgc ctcggccctg gtcttcgacg
       61 ccaccggata ctggcagggc aagcccgatc tgggccctgt cgaactgggt tccacggtcg
      121 gcgtcgcgcg ccccgcggcc ccgcagctgg atcgtttcgc cttcgcgtct ccccgcgcgg
      181 gccaggccac tctcaagctc cattcggcct cgggccgcct gctggcctct ttgccgctga
      241 ccgtacagga aggccgcaac cgcatggaga tccccgccgg gatccacccg ggagcctcgt
      301 tcgcgacggt ggatctggac ggaatccgtc tgcaccgggg agcggtggcg cgggtggagt
      361 gagggggctt tctactccgg ctctgcgatc ttcccgtgct ttcggcgcct ttccaggaag
      421 atgccgagtc ccaccaggct gcccccggtg aggagcagat ggaggaacca ggcctcgccg
      481 ctgttggcga cgacgaactg gaagtagaag gtgtagaggt tgatgaccag gaaggtgaac
      541 gcgtacttgc gcaggagctt ccagcccgtc ttgctggaga cgaccatcga aagcaccgaa
      601 agcccaaacc agaggatcga gaagacgagg cgctccgatc cacggcccga ccagatctgg
      661 tcgatgtcct cgtagtaccc gaacagggac atgaaccaca tcgccatgtt cgcgatcaag
      721 agtccgtaat gggcgtacac tcgcgagaag cggacccagc gccctcgcaa gcccaccgca
      781 tggacatagg ccagtcccaa cgtgacgacc gcgatggcga gataacgcac ggggtaggtc
      841 atgccgaggt agtaggcccc ccagtccgag aggtagcccg tctcggcgcc gaggaagaag
      901 aacaggttgg cgcaggcgta ccacaggacg agcggattgg ccaaggcgta ggcgagcccc
      961 accaacagcg cggcatcgac acccaccagg gacggccagt ttttgcttcc tgtggagtag
     1021 tggatcgcca gcacgatggt gagtccttgc agggccatgc cgccggcgag ctccaacgac
     1081 tcccccagga gaggcatccc cttggagagt ttgagccaac ggccaagcgc gatgaatccc
     1141 accacagtgc ccagcaatcc aagctcggac agcagccaga agtccagcat atccttgacc
     1201 agtatgaccg ttcccgtcat ggcggtgagc acgcccagga ccgtgaagat gcgcaccagc
     1261 ggcaggatgt cccacggagt ggtcggatac cgctccttga ggggcgcact ttgcccaggc
     1321 gtcaggatct gtcgagacgt gagctcgtcc agttccgcct ggatccccgc gtacagcttc
     1381 cttccggcgc tcatgaatgg gctcccttca tgcggaccgc ccggacgatc ccctccatgc
     1441 atcctcccag ggctagaaga accagtccgc ccaacagcag gtaggagcca agctcaagcc
     1501 tgtcccagaa cgcctcgtgg tagcgcgtga acacgttgat cgcgaagaag accacaccga
     1561 aaccgcgaat cattccgttg tgcagcctgg caccgagcag aatctgtccg acggtcgctg
     1621 ccgcgagcac gagactccaa accagctcgc cttccgatgt ttgccgccag atcgacagga
     1681 tcaagaggct catgttcagg tacaggagac ccagcgactc ccacaccttg tagaaccggc
     1741 ccgtgcgatc ggccaggcgc agttcgtggt acacgcccac gccgaacgcc agcagcgcga
     1801 acacgctcat cgcgcgtgga tcctggacat ggaaaccgta ggtggatcgc cccagcatcc
     1861 cgttccaggc cccgacccaa tggaacaatg acgccacgga taggagcagg agcatcgagt
     1921 tgtggtactt gtaggccaac accgccatga ccggtatgct cgtgccggca atcatgatca
     1981 gcacgtcttc cggtgccccc accgcctggg cgaacagcat catcccagag gtccacagca
     2041 ccgcccccag cgtaaggacg atcttcgacg aggttgcgta acgctcgcga atgtccccgg
     2101 caagccggat cccccaccag accagaccac atccaaccgc ggctgtcacg acagcgccga
     2161 aatgcaccga gttggacatg gcagcgatca ttcccatgat gccgaagaag gcgatcaatc
     2221 cccccgcgat tccgaggtag cggacaaacc ctgcaactcc gaatgcctcc acctgatagc
     2281 gctcgcgcag cgtttcgagg gtcttctgct cgatcacgcc atcggcgaac caatcggaga
     2341 tgtcgtcggc caatgcctgc ttcacggctc gcgtggtcat gtcgcacctc ttgcttgcgt
     2401 gatcatggta ttcactatgg cctccacagc ggctccgggc aggcttgccc tcatggcttg
     2461 gtcgcgggta ttgttccgta cccgcgtcat gacgtgggtg ttgtctcgca gcgtctcgct
     2521 catgacctgg cgcaggaacg tcagccggcc gatgtcgtcg
//
LOCUS       DAOSPL010000619         2560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40423_length_2560_cov_2.885030, whole genome shotgun sequence.
ACCESSION   DAOSPL010000619 DAOSPL010000000
VERSION     DAOSPL010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40423_length_2560_cov_2.885030"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /gene="tdh"
                     /locus_tag="PLQ54_11510"
     CDS             complement(<1..947)
                     /gene="tdh"
                     /locus_tag="PLQ54_11510"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOS93933.1"
                     /translation="MRALVKRDPAPGLWLEETTLPEYGINDVLIKVLRTGICGTDLHI
                     YNWDPWAQGAVPVPLVIGHEFVGRIVEVGSNVTDFRPGDIVSGEGHVVCGRCRNCLAG
                     RRHLCMDTKGIGVNRPGAFAEYLALPMTNVWYHDPAIPRNIQAIFDPFGNAVHAALSF
                     PVLGEDVLITGAGPIGCISAAVARHAGARYVVVTDVNPYRLDLARRMGATLAVDPRAE
                     SLADVQRKLGMKEGFDVGLEMSGNADAFRDMLANMAHGAKIAMLGIPDRDMAVDWNTV
                     IFNMLTIKGIYGREMYETWYKMTVMLQSGLDISPVITHV"
     gene            996..2516
                     /locus_tag="PLQ54_11515"
     CDS             996..2516
                     /locus_tag="PLQ54_11515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOS93934.1"
                     /translation="MADPRPAEQGEDGSTSAHALGYRDAVCPEVADELDSESRSAASR
                     GRDLTVGSVPRHLLVFSVPMLAQSAVQNAYAIVNGIWVGRGLGTDALAAITAGFPVFF
                     MLVAAAMGLTMATNILVAQAYGARDWDRVRHVVRNSVTLIALVGIGTLIVGELLAAPI
                     LVAMETPGHVLRLASSYLHIYLLTTPLNFGLFLLAACLRGAGDSKTPVWFQLGALVLN
                     TVLDPLLMFGWLGFPRLGLNGTAWASITSMSAAFIGLALYLHRTRHLVAPAWRRLGLS
                     RSMTWLTLSIGVPSMIQQAMVSVGAAVTVGLVNRFGEDAAAAFGAVVRVEQMALLPAL
                     TIGMAIASLAGQNIGARRFDRVREVLRWGVLLSGGFTLFVSLLAAVVPRPLVLMFTGE
                     PDVVELGVGYLRTVSPAYVCFAVMFAANGVVNGAGRTFATTLFTLISIWLVRVPVATV
                     LSARMDSVRGIWLGIVAGLVVGMLVSLGYYHSGRWRRPVVTRPFQKPAEARPDSPA"
BASE COUNT          384 a          792 c          896 g          488 t
ORIGIN      
        1 aacacgtgag tgatcaccgg agagatgtcg aggccactct ggagcatgac cgtcatctta
       61 taccaggtct cgtacatctc gcggccatag atgcctttga tggtgagcat gttgaagatg
      121 acggtgttcc agtcgacagc catgtcgcga tcggggatgc cgagcatcgc gatcttggcg
      181 ccatgggcca tgttggccag catgtcgcgg aaggcgtcgg cgttgcccga catctcgagg
      241 ccgacgtcga agccttcctt catcccgagt ttgcgctgga cgtctgccag cgattcggcc
      301 cggggatcaa cggccagggt ggcccccatg cgccgggcga gatccagccg gtaggggttg
      361 acgtcggtca cgacgacata gcgagcgccg gcatgcctcg ccacggcggc cgagatgcag
      421 ccaatgggcc cagcgccggt gataagtacg tcctcgccga gaaccggaaa cgacagcgca
      481 gcgtggacgg cgttgccaaa ggggtcgaag atggcctgga tgttccgggg gatcgctggg
      541 tcgtggtacc acacgttggt catcggtagc gccaggtact cggcgaaggc cccgggccgg
      601 ttcacgccga ttcccttggt gtccatgcac agatgccgcc gaccggccaa gcagttgcgg
      661 cagcggccac acacgacatg cccctcgcca ctgacaatat cgcccggccg gaaatcggtg
      721 acattgctgc cgacttcgac gatgcggccg acgaactcat ggccgatgac cagggggact
      781 gggacggcac cttgtgccca cgggtcccag ttgtagatgt gcaggtcagt gccgcagatg
      841 ccggtgcgaa gcaccttgat cagcacgtcg ttgataccgt actcgggcag cgtggtctct
      901 tcgagccaca acccaggagc ggggtcccgc ttgacgaggg cacggatggt gggcattgga
      961 cctcccgggg cgcgggcggg cgcctgaccc tgagtatggc cgacccgcgc ccggcggagc
     1021 aaggcgagga tgggtcgaca tcggcccacg ccttgggtta ccgtgatgcg gtgtgccctg
     1081 aggtcgccga cgagctcgac agcgaatccc gctccgccgc atcccgtgga cgtgacctga
     1141 ccgtcgggag cgtgccgcgc catctgttgg ttttctcggt gcccatgctg gcccagagcg
     1201 ccgtgcagaa tgcctatgcc atcgtcaatg gcatctgggt gggccgcggg ctgggcaccg
     1261 acgccctggc ggccatcacg gcggggtttc cggtgttctt catgctcgtg gcggccgcca
     1321 tgggtctgac catggccacg aacatcctgg tggcccaggc ttatggcgcc cgggactggg
     1381 accgcgttcg ccatgtggtc cgcaactcgg tgactttgat cgccctggtt gggatcggga
     1441 ccctgattgt gggcgaactc ctggcagcac cgatactggt ggcgatggag acaccgggcc
     1501 acgtactccg gctcgcctcc agctacctac acatctattt gctgacgact ccgctgaact
     1561 tcgggttgtt cctgctcgcc gcgtgcctgc gcggcgcggg tgattcgaag acgcccgtgt
     1621 ggttccagct gggtgcgctg gtgctcaaca cggtgctcga cccgttgctc atgttcgggt
     1681 ggctcgggtt cccccgactg ggactgaacg gcacggcttg ggcgagtatc acctcgatgt
     1741 ccgccgcctt catcggactc gcgctctacc ttcaccggac ccggcatctg gtggccccgg
     1801 cctggcggcg gctggggctt agccggtcca tgacgtggct caccctgagc attggcgtgc
     1861 ccagcatgat ccagcaggcc atggtgtcgg ttggggctgc ggtgacggtg gggctggtga
     1921 atcgcttcgg cgaggatgcc gccgcggcct ttggagcggt ggttcgggtg gagcagatgg
     1981 cgcttctccc ggcactgacc atcggcatgg ccatcgcgag tctggcgggg cagaacatcg
     2041 gagcgcgccg gttcgaccgc gtgcgcgagg tgctccgatg gggagtgctg ctcagcggag
     2101 gattcaccct tttcgtctcg ctcctggccg ccgtggtgcc ccgccccctt gtgctcatgt
     2161 tcaccggcga gccggacgtc gtggaactgg gcgtgggcta tctgcggacg gtgtctcccg
     2221 cctatgtgtg tttcgcggtg atgttcgccg ccaacggcgt ggtgaacgga gcgggtagga
     2281 cgttcgccac gaccctgttc acgctgatct ccatatggct cgtgcgcgtt cctgtcgcga
     2341 ccgtactgag cgcccggatg gatagcgtgc ggggcatctg gctggggatc gtggcggggt
     2401 tggtggtggg catgctggtg agcctggggt actaccattc cggccgctgg cggcgtccag
     2461 tggtgacgag gccgtttcag aagccggccg aggcgagacc cgactcgccg gcgtaggagg
     2521 tccactggca gcgccggttg aacccgaacg tacttgccgc
//
LOCUS       DAOSPL010000620         2560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40429_length_2560_cov_2.542515, whole genome shotgun sequence.
ACCESSION   DAOSPL010000620 DAOSPL010000000
VERSION     DAOSPL010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40429_length_2560_cov_2.542515"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..995)
                     /locus_tag="PLQ54_11520"
     CDS             complement(183..995)
                     /locus_tag="PLQ54_11520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOS93935.1"
                     /translation="MAGKIVYYAADIETTGLHAWRDDIVELAALRFENGEPTDSFCTL
                     VHARIPVTPDARNVHGIDDDDLVDAPPVGEAVRGLLAFVGQGPVALHHARFDLGFLSR
                     ELLAHGLQARVPIIDTCELARDLRPELPSRELGAVCERLGIRVSARHRAKGDAEATGR
                     VLWKMLGATRAGAAAKLADMSSRFQPWQPLSFSGLRQCKAIARLMKIAPPGSDVRFEY
                     SPEAGPTTTRSATVEFYSRRDENAYMVARCHLHQYERRTFRIDRITHPRAAE"
     gene            complement(999..2015)
                     /locus_tag="PLQ54_11525"
     CDS             complement(999..2015)
                     /locus_tag="PLQ54_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93936.1"
                     /translation="MARRCADLCQFVDESLYLPGADLWLDASRPRPRAFVSHAHTDHL
                     GLSPDGGSHALVLCTPETGRLIAALMPYGAPVPPIEALPLHHTIQCGGVRLTALPSGH
                     ILGGAMLLVESHRGRLLFAGDVRLSGSVTCPPAETVEADHLIIEDTFGALHASFAPAS
                     DGARRVLEFCRDAQDRGQTPVLVTTSNCGKAQDMVAALSAAGLRVGLQPKLFRCTEAY
                     RELGVPLTGYAKVRLSDRGGGVRCDEVDCILVTCGFLDYNPDLDHHVDNPSMALVSGW
                     AADDEAHHFDAAIPWSDHASREDLHEFVRRVNPSLVWTFAGEGALARELRAMGVKARH
                     LGSL"
     gene            complement(2091..2438)
                     /locus_tag="PLQ54_11530"
     CDS             complement(2091..2438)
                     /locus_tag="PLQ54_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93937.1"
                     /translation="MVLTFSDCVEYKSGKTEVDAHRIAHVFVRGVEIDFRPEESDEFA
                     PICEGLEAPYESDDGDRSDDSYIEVRIAREACPVLVRVRHVKETYWPGDGTTVGGTTL
                     CAVEVTAGPSEGE"
BASE COUNT          391 a          841 c          909 g          419 t
ORIGIN      
        1 ggggctctat cgccccctcc acccctgttg tgccatggcc ctcggggtac ctgaggcatg
       61 atgcacggaa tccttgcttc ctggggtgat cgggccccgc gatggcgggc gtacccggtg
      121 ccatgggggc gctgtcattg cccttgggca tcagcccgct ggggatggag cgcgacgccg
      181 gatcactccg ccgccctggg gtgggtgatg cgatcgattc ggaaggtgcg gcgctcgtac
      241 tgatgtaggt ggcacctcgc caccatgtag gcgttctcgt ctctccggga gtagaactcc
      301 acggtggcgc tgcgcgtggt ggtggggcca gcctcgggcg agtattcgaa acgcacgtcg
      361 gagccgggtg gcgcgatctt catcaggcgc gcaatcgctt tgcattggcg caggccgctg
      421 aagctcaacg gctgccatgg ctggaaccgg ctcgacatgt cggccagctt cgccgccgcg
      481 cccgctcgtg tggcgcccag catcttccag agcacgcgcc ccgtcgcctc ggcgtcgccc
      541 ttggcgcggt ggcgcgcgct gacgcggatc ccaaggcgct cgcacacggc gccgagctcc
      601 cggctgggca actccggcct gaggtctcgg gccagctcac atgtgtcgat gatggggacc
      661 cgggcctgga ggccatgtgc caggagctcg cgcgagagga agcccagatc gaagcgcgca
      721 tggtgcagcg ccacgggtcc ctggccgacg aaggccagga gcccacgcac cgcctctccc
      781 acggggggcg cgtctaccag gtcgtcgtcg tcgatgccgt gcacattccg agcatcgggt
      841 gtcacgggaa tgcgcgcatg gacgagggtg cagaaggagt cggtgggctc tccattctca
      901 aaccggaggg cggccagctc gacaatgtcg tcgcgccagg catggagacc cgtggtctcg
      961 atgtcggccg catagtagac gatcttgccc gccattcgtc agaggctccc gaggtgtctg
     1021 gctttgacac ccatggcgcg gagctcgcgg gccagggcgc cttcaccggc gaaggtccac
     1081 acgagggagg ggttcacgcg gcggacgaac tcgtggaggt cctcgcgcga cgcatggtcg
     1141 ctccacggga tcgccgcatc gaagtggtgt gcctcgtcat cggcagccca tcccgacacc
     1201 agagccatgg aagggttgtc gacatggtgg tccagatcgg gattgtagtc gaggaatccg
     1261 caggtaacca gtatgcagtc gacctcgtcg catcggacgc cgccgccgcg gtcggagagt
     1321 ctgaccttgg cgtagcctgt aaggggcacc ccgagctcgc ggtaggcctc ggtgcagcgg
     1381 aagagcttgg gctggaggcc tactcggagc ccggcggccg atagggccgc gaccatgtcc
     1441 tgggctttgc cgcagttgct ggtggtcacg agcacgggcg tctgaccccg gtcctgggcg
     1501 tcgcggcaga actcgagcac tcggcgggcc ccgtcggagg ccggcgcgaa ggaggcatgg
     1561 agggcgccga atgtgtcctc gatgatcaag tggtcggcct cgacagtctc ggccggcgga
     1621 caggtaacgg agccggacag gcggacgtca ccggcgaaca gcaggcggcc ccgatggctc
     1681 tcgaccagga gcatggcgcc gccgaggatg tgaccactcg ggagggctgt caggcgaacg
     1741 ccgccacact ggatcgtatg gtgtagcggc agggcctcga tgggaggcac cggagcgcca
     1801 tagggcatga gggccgcgat gagtctcccg gtctctgggg tgcacaacac cagggcgtgg
     1861 gatccgccgt ccgggctcag gccgaggtgg tcggtgtggg cgtggctcac gaaggcccgg
     1921 gggcgtggcc gcgaggcatc cagccacagg tcggctccgg gcagatagag tgactcgtcg
     1981 acgaactggc agagatccgc acaacgccga gccatagcag gcgatcactt cccgtgctcg
     2041 gcgcattcgc cggccgggca gtgcgggcct gcgtcgggcg gcagctccgg tcactcgccc
     2101 tcggacggtc ccgccgtcac ctcgacggcg cagagcgtgg tgccgccgac tgtcgtgcca
     2161 tcgcccggcc aataggtctc cttcacgtgc cggacgcgca caaggaccgg gcaagcctcg
     2221 cgcgcgatgc gcacttcgat gtaggagtca tcggagcggt cgccgtcgtc gctctcgtag
     2281 ggcgcctcca ggccctcgca gatcggcgcg aactcgtcgc tctcctccgg gcggaagtcg
     2341 atctcgacgc ccctgacgaa aacgtgtgcg atccggtggg cgtcgacctc ggtcttgccg
     2401 gacttgtact cgacgcagtc gctgaaggtg agaaccacgg gcttgtcgcc cgccctgcgc
     2461 cgccgctact ccccggccca actggccctc ctccagctgg tgtattacct tcagccgatc
     2521 ccgctcccga gtgctcatct gcagctccat gtcctgtcat
//
LOCUS       DAOSPL010000621         2558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40472_length_2558_cov_3.703156, whole genome shotgun sequence.
ACCESSION   DAOSPL010000621 DAOSPL010000000
VERSION     DAOSPL010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40472_length_2558_cov_3.703156"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="PLQ54_11535"
     CDS             complement(<1..1221)
                     /locus_tag="PLQ54_11535"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HOS93938.1"
                     /translation="MTESHADRFARHAAPGRRLRGLQLTDLVDVQTLQEAQERFAELG
                     RVGIVIRGPDGQMITQPSCLNEFCSALLCHPELSRQCQASHNEVPAEPGHEHGVQAYA
                     CHAGLRQFAAPIVVRGRTYGTIIVGPVADQEMDPATLHRLAGQLGLPVDEVRRQAATV
                     RRWSDQEAGILVRGLEMTARNITELCLAGYDLRCTVEELRLVQEVGHELVADRSLEDT
                     MSLIARRITGIFGAKACLIRLFETDRGTTKVLASHGLSQEYLQKGPVGVATELERVAI
                     EGAVSVIEDIEKDERFVYRDAAREEGLRSAIYAGLICRGKALGGLRLYFGEPRETDES
                     ELRLVRALADQAALAIENARLIDSLRRANQRLREAYELMHGAQDKLRQTERLAALGEM
                     ASGIAHEVKNPQAAV"
     gene            complement(1312..2208)
                     /locus_tag="PLQ54_11540"
     CDS             complement(1312..2208)
                     /locus_tag="PLQ54_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93939.1"
                     /translation="MSVVIALAGDTLRSCGRAWILVGLVTVLALAAATTVAVIAHVPA
                     EYMKAAAGLVEQGAVPSLPGDTASDERQTKAAVIQDLTASEYELVMKDTARAAGLRHV
                     VGMGGALLAALIGVLVNETRSGRIGPIVARAIPRRTVVLGQMVGGACALTMATALLAV
                     ALMLADAAVVGRAEPRVLPAAGLILPNLMVCLTLVYALATVMHPVGAAGVGLLVCAAV
                     GQIGAIYRSLTAAEVHGLVVDATRLAYRLLPHLGDLKTAAEDYLIEARVHIPIGPAIG
                     TDAIYTLLLTLAACWLFSRRQV"
     gene            complement(2205..>2558)
                     /locus_tag="PLQ54_11545"
     CDS             complement(2205..>2558)
                     /locus_tag="PLQ54_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS93940.1"
                     /translation="SSHMLSEVEMTCTRVAIMDAGRIVRAGTVDELTRTVHYVHIGVT
                     TVSDGLLADLRSALANVREMQGYIVGEIRSESDLDPIPGIVERHGARLRYLAMAHETL
                     EDAFLRTVGVKGASG"
BASE COUNT          388 a          876 c          910 g          384 t
ORIGIN      
        1 gacggcagcc tgggggttct tgacctcatg ggcgatgccg ctggccatct ctcccagggc
       61 cgccaggcgc tcggtctggc gtagtttgtc ctgggctccg tgcatgagct cgtaggcttc
      121 acgtaggcgc tggttcgcgc ggcggaggct gtcgatgagg cgggcgttct cgatggcgag
      181 agccgcctgg tcggccaggg cccgcacgag gcgcagctcg ctctcgtccg tctcgcgggg
      241 ctcgccgaag tagagccgga gcccgcccag ggccttgccg cggcagatga ggcccgcgta
      301 gatggcggag cgaagccctt cctcgcgggc cgcatcccgg tagacgaagc gctcgtcctt
      361 ctcgatgtcc tcgatcaccg atacggcgcc ctcgatggcc acgcgctcga gctcggtggc
      421 cacgcccacc gggcccttct ggaggtactc ctggctcagg ccgtgcgagg ccagcacctt
      481 ggtggttccc cgatcggtct cgaagaggcg gatgagacac gccttggcgc cgaagatgcc
      541 ggtgattcgg cgggcgatga ggctcatggt gtcttcgagg ctgcgatcgg ccaccagttc
      601 gtgtccgact tcctggacga gccggagttc ctccacggtg catcgcagat cgtagcccgc
      661 caggcacagc tcggtgatgt tgcgggcagt catctcgagg ccgcgaacca ggatgccggc
      721 ctcctgatcg gaccaccgtc gcacggtggc tgcctgacgc cggacctcgt cgaccgggag
      781 gccgagttgg cccgccagac ggtgaagggt ggcggggtcc atttcctggt cggccaccgg
      841 cccgacgatg atggtgccgt aggtgcgccc ccggaccacg atgggggcgg cgaactgccg
      901 caggcccgca tggcacgcat aggcctggac accatgctca tgccccggct cggccggcac
      961 ctcgttgtgg gaagcctggc actggcgtga gagctccggg tggcacagga gggccgagca
     1021 gaactcgttg aggcaacttg gctgtgtgat catctggccg tcggggcccc ggatgacgat
     1081 ccccacacgg cccagctcgg cgaatcgctc ctgggcctcc tggagggtct gtacatcgac
     1141 caggtcggtg agttggagcc cgcgcaagcg ccgccccggg gccgcgtggc gagcgaaacg
     1201 atcggcatgg ctttctgtca ttcaagactc ccctggcggt gccgtcgcgc tgttcgggcc
     1261 ggcggagcgg gtgggggcgg aacccgggct gcgttcggcc ggcacgctca gctacacctg
     1321 ccgccttgag aataaccagc aggctgccag tgtgagaagg agggtgtaga tggcgtccgt
     1381 gccgatggcc ggcccgatgg ggatgtggac ccgggcttcg atcaggtagt cctcggccgc
     1441 ggtcttgagg tcgcccaggt gcggcaggag ccgataggcg agccgggtgg cgtcgaccac
     1501 caggccgtga acctcggcag ccgtaaggga ccggtagatg gcgccgatct gccccacggc
     1561 ggcacagacc agcagcccca cacccgcggc gcccacgggg tgcatcacgg tggcgagcgc
     1621 gtagacgagc gtgaggcaca ccatcaggtt gggcaggatc aggcccgcag cgggcagcac
     1681 gcgtggctcg gcccgcccca ccaccgcggc atcggccagc atcagcgcca ccgcaaggag
     1741 tgcggtcgcc atggtcagcg cgcaggcccc acccaccatc tggcccagga ccacggtgcg
     1801 gcgcgggatg gcacgggcga cgatgggccc aatccgcccc gagcgcgtct cgttcaccag
     1861 aaccccgatg agggccgcca ggagcgcccc gcccatgccc accacgtgcc ggagtccggc
     1921 cgcacgggcc gtgtccttca tcaccagctc gtactcggac gcggtcagat cctggatgac
     1981 cgccgccttg gtctgccgct catcgctcgc cgtgtcgccg gggagcgacg ggacggcgcc
     2041 ctgctccacc agacccgccg cggccttcat gtactccgcg gggacatgag cgatgaccgc
     2101 caccgtcgtg gccgccgcca gggcgaggac ggtcaccagg ccgacgagga tccaggcccg
     2161 gccgcagctc cgcaaggtat ccccggccag ggcgataacg acgctcatcc cgaggccccc
     2221 ttcacaccca cggtacggag gaaggcgtcc tcgagcgtct cgtgagccat ggccaggtac
     2281 cggagacgcg ccccgtggcg ctcgacgatg ccagggatcg ggtccaggtc ggactcggag
     2341 cggatctccc ccacgatgta cccctgcatc tcgcgcacgt tggcgagcgc ggatcggagg
     2401 tccgccagaa ggccatcgct gacggtcgtc acaccgatgt ggacgtagtg gacggttcgc
     2461 gtcagctcgt cgacggtgcc ggcgcgcacg atccggccgg catccatgat ggcgacgcgc
     2521 gtgcaggtca tctcgacctc ggagagcatg tgggagct
//
LOCUS       DAOSPL010000622         2558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40482_length_2558_cov_2.648821, whole genome shotgun sequence.
ACCESSION   DAOSPL010000622 DAOSPL010000000
VERSION     DAOSPL010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40482_length_2558_cov_2.648821"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PLQ54_11550"
     CDS             complement(<1..704)
                     /locus_tag="PLQ54_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93941.1"
                     /translation="MFRVPWWIGFVPILALWAAPTQAIEETGLYSRRPTWQATLRASW
                     ANLQRLEDQERAAAPCVDENGVTLGPWYAIGPFDNTGGQGFAAAYPPETELLLDRAYT
                     GAEGVEAVWVRMDRFVDGQVNDLRGLYPSPDAGIAYLYRVITAERAVEIRGYFGSDDG
                     LAVWLNGDKLISNDVPRGPGPDQDQAALALRQGENHLLLKIANRLGGWAFYFHTKPGG
                     GPPQGNPEHVRRLSAI"
     gene            850..1938
                     /locus_tag="PLQ54_11555"
     CDS             850..1938
                     /locus_tag="PLQ54_11555"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS93942.1"
                     /translation="MTPRERFNAVMFYEEFDRVPVWHWTEWPETQQRWAREGLPADGN
                     RYEYFGCEPMTAGIPLHLGLLPGFDEETVEETDEYRIFRQADGVIAQHWKAKSCIPHY
                     VDFILKDRQSWPEYKKRLQPDPARLPQDLPRIATDLEATNLPIAVPTASLTGWIRDWM
                     GVVGFSYIQHDDPDLLGEMVDTIADLVCWGLDQVLPHCHADLGWGWEDICGKNGPLVS
                     PPIFEEYVVPGYRKISDKLAEHGTQLHLTDCDGDIRALVPGWLRGGVNVMFPIEIGTW
                     HADPMAFRQQYGRELRVIGGIDKLEIAKGPEATKAEIRRRLPLMRDGGFIPLPDHLIV
                     PETSLADYRFYIECMKRLDLSKENGGLD"
     gene            1938..>2558
                     /locus_tag="PLQ54_11560"
     CDS             1938..>2558
                     /locus_tag="PLQ54_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS93943.1"
                     /translation="MRRVTRREFVGYGVAGATAGLTLRARGADMQRWGFIGTGGRGTY
                     LMGQALAIPEVDVTAACDINEAHLNGALELVEKARGHVPQGYSSGPKAYRELLKRDDV
                     DAVVIATPVPLHARMAADAMRAGKHVLSEVGGAYTLDDCWDIVDAQKETGCTYMLAEN
                     CCYYRQNLMVLDMIAQGLFGDLTYAECGYVHDCRWLYFDPSGNLTWR"
BASE COUNT          463 a          839 c          855 g          401 t
ORIGIN      
        1 cagatggccg agagacgccg cacgtgctcg ggattcccct gaggcggccc gcccccgggc
       61 ttggtgtgga agtagaaggc ccacccgccc aggcggttgg cgatcttgag cagcagatgg
      121 ttctcgccct ggcgtagcgc aagcgctgcc tggtcctgat ccggtcccgg cccgcgaggc
      181 acatcattgc tgataagctt atcgccattg agccaaacgg ccaagccgtc atcactgccg
      241 aagtagccgc ggatctcgac tgcccgttcg gcggtgatga cgcggtacag gtacgcgatg
      301 cccgcatccg gactggggta gagcccccgc aggtcgttga cttggccgtc gacgaagcgg
      361 tccatgcgca cccagactgc ctcgacgccc tcggcacccg tgtaggccct gtccagcaag
      421 agctcggtct cgggcgggta agccgcggcg aagccctggc cgccggtgtt gtcgaagggc
      481 ccgatggcgt accagggccc gagcgtgacg ccgttctcgt cgacgcaggg cgcggcggcc
      541 cgctcctggt cctccagcct ctgcagattg gcccacgacg cccggagtgt ggcctgccaa
      601 gtcggccggc gggagtagag accggtctcc tcgatggcct gcgtcggagc cgcccacagg
      661 gccagtatgg gcacgaaacc gatccaccag ggcactcgga acacagcaca ctcccccgta
      721 cgtggccgga aggcgaaccg acccgagtgt accggagacg accccgggcc acaacggcca
      781 gcggaacgcg aggcattgca gagcctgagg ccgaacctgg agacacccca catccaagga
      841 gactgcccga tgacgcccag ggaacgcttc aacgccgtca tgttctacga ggagttcgac
      901 cgagtccccg tgtggcactg gaccgagtgg cccgagaccc agcagcgctg ggcgcgtgag
      961 ggactgcccg ccgacggtaa ccgctacgag tacttcggct gcgaaccaat gaccgcgggc
     1021 atcccactcc atctggggct cctaccgggg ttcgacgagg agaccgtcga ggaaacggac
     1081 gagtaccgga tcttccgtca ggccgacggc gtcatcgcgc agcactggaa ggccaagagc
     1141 tgcatccccc actacgtgga cttcatcctc aaggaccggc agagctggcc cgagtacaag
     1201 aagcgccttc agcccgaccc ggcgcggctg ccccaggacc ttcccaggat cgcgaccgac
     1261 ctggaagcca cgaacttgcc cattgccgtg cccaccgcga gcctcacggg atggatccgc
     1321 gactggatgg gcgtcgtcgg gttctcctac atccagcatg atgatcccga cctgctgggt
     1381 gaaatggtcg acacgatcgc ggacctggtg tgctgggggc tcgaccaggt gctgccccac
     1441 tgccacgcgg atctcggatg gggttgggag gacatctgcg gcaagaacgg tccgctcgtg
     1501 agcccgccga tcttcgaaga gtacgtggta cccggctacc gcaagatctc ggacaagctg
     1561 gccgagcacg gcacccaatt gcacctgaca gactgcgatg gcgacatccg cgccctggtt
     1621 cccggatggc tgcgtggcgg cgtgaatgtg atgttcccca tcgagatcgg cacctggcac
     1681 gccgacccca tggcctttcg gcagcagtac gggcgcgagc tgcgtgtcat tggcggcatc
     1741 gacaaactgg agatcgccaa ggggccggag gcgacgaagg ccgaaatccg tcggcggctg
     1801 ccgctgatgc gcgatggagg gttcattccg ctgcccgatc acctcatcgt gcccgagacc
     1861 tcactcgccg actaccgctt ctatatcgag tgtatgaagc gcctcgacct gtcgaaggag
     1921 aatggggggc tcgactgatg cgtcgcgtca cgcgcagaga gttcgtaggc tatggcgtgg
     1981 ccggtgcgac tgccggcctg acactgagag caaggggtgc cgacatgcag cgttgggggt
     2041 tcatcggaac cggtggccgc gggacgtacc tgatggggca ggcgctggcg atccctgagg
     2101 tggacgtcac cgcggcctgc gacatcaacg aggctcacct caacggggcg ctcgaactgg
     2161 tcgagaaggc gcgcggccat gttccccagg gctattcgtc gggccccaag gcgtaccggg
     2221 agctcctcaa gcgcgatgac gtggacgcgg tggttatcgc cacccctgtg ccgctccacg
     2281 cgcgaatggc ggccgacgcc atgcgggcgg gcaagcacgt gctgagcgag gtcggcggcg
     2341 catacacgct cgacgactgc tgggacatcg tggacgccca gaaggagact ggctgcacgt
     2401 acatgctggc cgagaactgc tgctactacc gccagaacct gatggtcctc gacatgatcg
     2461 cccagggcct gttcggcgac ctgacttacg cggagtgcgg ctatgtgcat gactgccggt
     2521 ggttgtactt cgacccgtcc ggcaacctga catggcgg
//
LOCUS       DAOSPL010000623         2557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40502_length_2557_cov_3.084333, whole genome shotgun sequence.
ACCESSION   DAOSPL010000623 DAOSPL010000000
VERSION     DAOSPL010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40502_length_2557_cov_3.084333"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="PLQ54_11565"
     CDS             complement(<1..781)
                     /locus_tag="PLQ54_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOS93944.1"
                     /translation="MRKRGFTLIELLVVIAIIAILAAILFPVFAKARAKARQSSCASN
                     LKQIGLATAQYISDYDSTYPLATGVLGADALGDYMLPIPMWSGYPSLSTIPHVAIAPY
                     MKNWEIMHCPSGQRTPYYDPEAGQPTTSISYSFNPLLEAYPESAIVEPATTISWWEDG
                     VNAFGNADIFRNPWRSGPTTVPSYPNRWQTGSGEQAQMWASSLTGGIWIHNEGINIVY
                     CDGHVKWAKCPGEGSPYAAVDGSGNFTWYWWNGYGPWYHSPD"
     gene            complement(1354..1803)
                     /locus_tag="PLQ54_11570"
     CDS             complement(1354..1803)
                     /locus_tag="PLQ54_11570"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOS93945.1"
                     /translation="MNPEQALVTSQLTHMRASLERLHRLAALDDEEFYASPDNAALAN
                     HHLQRAIQAVLDIGRHLTIRRRLGRTSSYAEIIYRLYEHHLIDPDLGERLVDLAHMRN
                     RFVHVYWEVSAQEVRQVLRERLEDLEEFIRQIVRFLDKEAPPPDARG"
     gene            complement(1793..2230)
                     /locus_tag="PLQ54_11575"
     CDS             complement(1793..2230)
                     /locus_tag="PLQ54_11575"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOS93946.1"
                     /translation="MDPQTRQTIARLCQKHGVGLCYLFGSHVLPDDPDEMADVDIGIV
                     PRGWPAERSDLAQWLSLRKALEPLFAPRLVDLVQLDRAGTHLQSEAVTSGQLLYSADE
                     ELRVAFEDRVLRDWHDFAWVVEANYRDIRKEILGEEGRCTDEP"
     gene            complement(2276..>2557)
                     /locus_tag="PLQ54_11580"
     CDS             complement(2276..>2557)
                     /locus_tag="PLQ54_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011019838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93947.1"
                     /translation="SFHQGETPRELEARILQDADRLDAMGAVGIARAFMRAGEQGRSL
                     AAAVDHFHEKLLRLQDQMHTPAARAMAARRHQILVRFLEDLAAESPKLT"
BASE COUNT          424 a          766 c          932 g          435 t
ORIGIN      
        1 ggtcgggcga gtggtaccag gggccgtagc cgttccacca gtaccaggtg aagttgccgc
       61 tgccatccac ggcggcatag ggactgccct cacccgggca cttggcccat ttcacgtggc
      121 cgtcgcagta gacgatgttg atcccctcgt tgtgaatcca gatgcctccg gtgaggctgg
      181 acgcccacat ctgggcttgc tcgccggagc cggtctgcca ccggttggga tagctgggaa
      241 cggtggtggg accgctgcgc cagggattgc ggaagatgtc cgcattgcca aaggcgttga
      301 ccccatcctc ccaccacgag atggttgtgg cgggctcaac aatggcgctc tccgggtagg
      361 cttcgagcag ggggttgaag ctgtagctga tggacgtcgt cggctgtccg gcctccgggt
      421 catagtaagg ggtcctctgg ccggacgggc agtgcatgat ctcccagttc ttcatgtagg
      481 gtgcgatggc gacgtgaggg atcgtgctca gcgatggata gccggaccac atgggaatgg
      541 ggagcatgta gtcgcccagg gcgtcggcgc cgagcacccc cgtagccagg ggataggtgc
      601 tatcgtagtc gctgatgtac tgggcggtgg caagcccaat ctgcttgagg ttgctggcgc
      661 aggatgactg gcgtgccttg gctcgcgcct tggcaaagac ggggaacagg atggccgcca
      721 ggatcgcgat aatggcgatc acgacgagca gctcaatcag tgtgaagcct ctcttgcgca
      781 caataggacc tccttgagta cggtgcgggg aaccgcaagg cgacggagcc gactcagggc
      841 cgacggctgc tgtccgttcg atagctactt gcctggcggc gtgagtttct gcgcctgtgt
      901 gctcagcgct tggcctgttt tggcgccttc aggggcctgg ccggcctccg aagccttcgg
      961 cttgccacac ccaatgcaga gcacggagag gagacacaga aaaaggcagg aagcaagaaa
     1021 cttatgcaca acaatacacc ccccggagac tgaggacacg cgtcgcgagg gcgaagtcaa
     1081 ggcgccacaa caaccgacgg gctcgacagc caagctgccg ggccgtcgcg gctctgggga
     1141 gagctgcggc ggcgtacgcg tcccgtcagt agtgatacca taaatcgttg cgggtgtgaa
     1201 gtcgtgacgg ccagcagtcc tgcagaccgc gcgaatcctg gcgtccgcgg ccgccaggcg
     1261 ttcgcccaag gcaaggggca ccggcgagcc gtgagcggcg ggcagcggct cgccgatcgg
     1321 ttcgggccca caagcccggc gccccggctg acgctatccc cgcgcgtcgg gcgggggggc
     1381 ctctttgtcg aggaagcgga cgatctggcg gatgaactcc tcgaggtcct cgagtcgctc
     1441 gcggaggacc tggcggacct cctgggcgga gacctcccag tagacgtgaa cgaagcggtt
     1501 tcgcatgtgc gccagatcga cgaggcgctc gccgaggtcg gggtcgatca ggtggtgttc
     1561 gtagaggcgg tagatgatct cggcgtagga ggacgtgcgg cccaggcgtc gccggatcgt
     1621 caggtgccgc ccaatgtcga ggacggcctg gatggcgcgc tggaggtgat ggttcgccag
     1681 agcggcgttg tccgggctcg cgtagaactc ctcgtcatcc agggcggcca gacggtgcag
     1741 gcgctcgagc gaggcgcgca tgtgggtcag ttgggatgtg accagggctt gttcagggtt
     1801 catcggtgca gcggccctct tctcccagga tctccttgcg gatgtcgcgg tagttggcct
     1861 cgaccaccca ggcgaagtcg tgccagtcgc ggagcacgcg gtcctcgaag gcgacgcgta
     1921 gctcctcgtc ggccgagtag agcagttggc cggacgtgac ggcctcgctc tgcagatggg
     1981 ttcccgcccg gtcgagttgg acgagatcga cgaggcgggg cgcgaaaagc ggctcgaggg
     2041 ccttgcgcag gctgagccac tgggcgaggt cgctccgctc cgcgggccag ccgcgaggga
     2101 caatgccgat gtcgacgtcg gccatctcgt cggggtcgtc gggaagcacg tgcgagccga
     2161 acaggtagca caggccgacg ccgtgcttct ggcacaggcg agcgatggtc tgtcgggtct
     2221 ggggatccat cggcgtgctc ccagccgggc agggcaccca taccccgcgc ggctctcagg
     2281 tgagtttcgg gctctccgcg gccaggtcct cgaggaagcg gaccaggatc tggtgacggc
     2341 gagccgccat ggcgcgggcg gcgggcgtgt gcatctggtc ctgtagccgc aacagcttct
     2401 cgtggaaatg gtcgacagcg gccgccaggg agcggccctg ctcgccggcg cgcatgaagg
     2461 cccgggcgat gcccacggcg cccatggcat ccaggcggtc ggcatcctgg aggatccgcg
     2521 cctcgagctc tcgtggcgtt tcgccctgat ggaagct
//
LOCUS       DAOSPL010000624         2551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40616_length_2551_cov_3.041266, whole genome shotgun sequence.
ACCESSION   DAOSPL010000624 DAOSPL010000000
VERSION     DAOSPL010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40616_length_2551_cov_3.041266"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLQ54_11585"
     CDS             complement(<1..510)
                     /locus_tag="PLQ54_11585"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOS93948.1"
                     /translation="MRRVCWLIKWVADRGVALVALLLLGPVLLAVAVVIRRSMGAPVL
                     FRQVRPGFREKPICVLKFRTMREAVNGEGRPLPDAERLTPVGRFLRQTSLDELPQLWN
                     VLRGDMSLVGPRPLFMHYLERYTPEQRRRHEVMPGMTGWAQVNGRNAISWEDRFALDV
                     WYVDNWSLGL"
     gene            complement(685..1155)
                     /locus_tag="PLQ54_11590"
     CDS             complement(685..1155)
                     /locus_tag="PLQ54_11590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS93949.1"
                     /translation="MESLARELRIAHVTFTGTLSKTEVAECLAAADVCVAILQDIPMF
                     RTTYPNKVFDYMAAGRPTVLAIDGVIRQVIEEAGAGVYARPGSADALAKAIRMLATDP
                     ERARAMGRAGRAHVVEHFNRADQAAEFARVVEGLIAPHSPQDDSGAGSGAELSP"
     gene            complement(1131..1958)
                     /locus_tag="PLQ54_11595"
     CDS             complement(1131..1958)
                     /locus_tag="PLQ54_11595"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS93950.1"
                     /translation="MRILLIHQNFATPAEGGGTRHFELGRHCSQRGHEFVVVTSPLSY
                     LSGRQTATEDPPGSTEQVVEGVRIRYAYAHPSLHRSFVWRLFSFISFTLSAIRTALGV
                     GSVDLVVGTSPPLPQALSAWYVSRRRRCPFLLEIRDLWPEFAIAMGVLTNPVLVGLAR
                     CFERFLYRRAHLILVNSPAYRDYLTERGVPAAKIRLIPNGVDPAMFDPDADGAEARAR
                     WGLEGRFVVVYAGALGMANDIATLLRAVAQLEDIPHLHLLLVGTGRSAPTWSPWLES"
     gene            complement(1955..>2551)
                     /locus_tag="PLQ54_11600"
     CDS             complement(1955..>2551)
                     /locus_tag="PLQ54_11600"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOS93951.1"
                     /translation="SGVRRSPCGLLACLEGSHDGYSRLHPAVSYRRAVILLPGEGWLV
                     LDRMRSAGPHSYRLHWLCPDLPHECEPREAKLCLTTPSGPYAVQMGVLPVDGAAKAEF
                     RCSLVRADPESPRGWRSRYYLDREPALSFDLTVEAHSIILWSLLSPGLWPVAPTSSRS
                     LAIEGPGGVARVSAGAEDGPLVARVEWVGTVEDSLEAS"
BASE COUNT          489 a          893 c          798 g          371 t
ORIGIN      
        1 caacccaaga gaccagttgt ccacatacca gacgtccaga gcaaaccgat cttcccagga
       61 tatggcgttg cggccgttca cctgggccca acccgtcatc cccggcatga cctcatgccg
      121 acgtcgctgc tccggcgtgt agcgctccag gtagtgcatg aacagcggcc gaggccccac
      181 cagcgacatg tcgcccctca ggacattcca cagctgcggg agctcgtcca gactcgtctg
      241 gcgcaggaag cgacccacgg gcgtcaaccg ctccgcatcg ggcagtgggc gcccctcgcc
      301 gttcactgcc tcccgcatcg tgcgaaactt cagaacgcag ataggtttct cccggaaccc
      361 cggtcgcacc tgtcgaaaca acacgggagc acccatactc ctgcggatca ctacggccac
      421 ggccagcaaa acgggcccca gcagcagaag tgccactagg gccacgccac gatcggctac
      481 ccacttgatg agccagcaga cccggcgcaa tggcgcctcc cccgtgctct gggaataccc
      541 agcgggcccg ggcccgctgg tgcctccgcc ggaacagacg ccgaggcggc cacccacact
      601 ccctttccgg cgacgccatc ggcgctcagg catttggcgt tacaggctac tctgtgcggt
      661 taccgtagtt taccaccgcg cccactatgg cgacagttcg gcgccgctgc ccgcaccaga
      721 gtcgtcctgg ggcgagtgag gtgcgatcag tccctcgacg acccgagcga actcagcggc
      781 ctggtccgcc cggttgaagt gctccacgac atgggctcgg ccggcgcggc ccatggcccg
      841 cgcccgctcg ggatcagtcg ccagcatccg gatggccttc gccagggcat cggcagaccc
      901 cggcctcgcg tagactccgg cacccgcctc ttcgatcacc tggcgaatga ccccatcgat
      961 agccaggact gtcggccgcc ccgccgccat gtagtcgaag accttgttgg ggtacgttgt
     1021 gcgaaacatg gggatgtcct gcagtatggc cacacacaca tcggcagcgg caaggcactc
     1081 ggcgacctcg gtcttcgaca gcgttcccgt gaaggtcaca tgggctatcc tcagctctcg
     1141 agccagggac tccaagttgg cgcgctcctt cccgtcccca ccaacagaag atgcagatga
     1201 gggatgtcct cgagctgggc gacggctctc aggagggtgg cgatatcgtt ggccatgccc
     1261 agggcgcccg cgtataccac cacaaaccgg ccttccagcc cccaccgggc gcgcgcctca
     1321 gctccgtcgg catccgggtc gaacatggcc gggtccacgc cgttggggat caaccggatc
     1381 ttcgccgcgg gcacgccgcg ctcggtcagg tagtctcgat aggcgggcga gttcacgagg
     1441 atcaggtggg cccggcggta caggaaacgc tcgaagcagc gtgcgagccc aacgagcacc
     1501 ggattcgtga gaacacccat ggcgatggcg aactcgggcc acagatcccg aatctccaag
     1561 aggaacgggc accggcggcg ccgcgagaca taccaggccg acagggcctg aggcaacgga
     1621 ggcgacgtgc ccacgaccag atccacagaa ccgacaccca aggccgtgcg aatggcactg
     1681 agggtgaatg atataaacga aaacagtcgc cacacgaagc tgcgatgtag tgacggatgc
     1741 gcgtacgcgt agcggatgcg cacaccctcg accacctgtt cggtggaacc cggtggatct
     1801 tccgtcgcgg tttggcggcc gctgagatag ctgagcgggc tcgtcacgac gacgaactcg
     1861 tgcccccgct ggctgcagtg ccggcctagc tcgaagtgcc gggtcccacc tccctcggcg
     1921 ggcgtggcga agttctgatg aatcaacagg atcctcatga tgcttccaac gagtcctcca
     1981 cagtgcccac ccactcgacc ctcgcgacca acggcccgtc ctccgcgcct gcgctgaccc
     2041 gcgcgacccc tcccggtccc tcgatggcga gcgagcggct ggaggtgggc gccacaggcc
     2101 acaagcccgg gctgaggaga gaccagagga tgatggagtg cgcctcgacc gtcaagtcga
     2161 acgaaagcgc cggctcccgg tcgaggtagt agcgggaccg ccatcctcgc ggtgactccg
     2221 ggtcggcccg aaccagactg caccggaact ccgccttcgc agcgccatcg accggaagca
     2281 ctcccatttg gacggcgtag gggcctgacg gggtcgtcag gcacagcttg gcttcgcggg
     2341 gttcgcactc gtgaggcaag tcggggcaca gccagtgcaa ccggtatgag tgagggccgg
     2401 ccgacctcat gcggtcgagg accagccagc cctcgccggg cagcaagatc acggcgcggc
     2461 gataggagac agccggatgc agacgactgt agccgtcgtg cgacccctcg agacacgcca
     2521 gaaggccgca aggcgaacgg cggacaccgc t
//
LOCUS       DAOSPL010000625         2548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40691_length_2548_cov_2.538307, whole genome shotgun sequence.
ACCESSION   DAOSPL010000625 DAOSPL010000000
VERSION     DAOSPL010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40691_length_2548_cov_2.538307"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PLQ54_11605"
     CDS             <1..1011
                     /locus_tag="PLQ54_11605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOS93952.1"
                     /translation="SDLRMPGMDGIEFLSRVRQAAPDTVRIMLTGNADLSTAIDAVNE
                     GAIFRFLTKPCPMDTLVTTIRAGAAQYRLVTAERELLEKTLTGSIKVLTDVLSLANPA
                     AFGRAARVRRIVYSVAEAMGLADAWLYGIAAMLSQVGCVSLPEVILHKLNSGQPMDAE
                     EQRLFEEHPGIGSELLANIPRLEPVAKIVAYQEKNYDGTGVPADDVKGDEIPLGARVL
                     HAALHYDALLSAGKSSTAAVAAMQRRSGHYDPAIVSALAEVAGAESSWEERLVSAWDL
                     AAGMSLLDDVTTPTGALVVCRGQEVTPSIRARLISLVRRAAVREPFRVLAPVTPMRGG
                     DV"
     gene            1097..1285
                     /locus_tag="PLQ54_11610"
     CDS             1097..1285
                     /locus_tag="PLQ54_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93953.1"
                     /translation="MKGEWRPVGERTEFPGVSIRHTDSRRPPLLPVLLDALKLLSMAW
                     EVLPPRQQPADETTQAEG"
     gene            complement(1376..>2548)
                     /locus_tag="PLQ54_11615"
     CDS             complement(1376..>2548)
                     /locus_tag="PLQ54_11615"
                     /inference="COORDINATES: protein motif:HMM:NF016071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HOS93954.1"
                     /translation="LPECVEAEPNDGLERAQSVRLPVGVSGRIERAGDVDVYRFDGRA
                     GETVVAEVSARRLGSELDSLVRLMDGAGKIIAWNDDSPDPGAGLITHHADSYVRAELP
                     ADGPYFVRVSDVQQHGGERYGYRLRLSGPRPDFALRVTPSSLSIPAGRSAAATVHAIR
                     KDGFDGEIEVSLADAPAGYALAGGRVPAGLDSIRITVSAPKQPFGRPVSLRLQGTAAI
                     AGQMVIHEAVPAEDMMQAFAYRHLVPSMELLATTTGLGGRVPTVSWADAGPVRVPAGG
                     SVQVRVNSPAGPLLSMVRLSLSEPPAGIALDGVEPVPGGLLLTLSAGSDSPPAGYVDN
                     LIVELYAELETKAQGGKPATTRRVAAGVLPALPFEIVAQAEAADRAPRASRAGWRR"
BASE COUNT          418 a          932 c          817 g          381 t
ORIGIN      
        1 tcggaccttc gcatgccggg catggacggc atcgagttcc tctcgcgtgt ccggcaagcg
       61 gcaccggata cggtgcgcat tatgctgacc gggaacgcgg acctgagcac tgccatcgac
      121 gccgtcaacg aaggagcgat cttccgcttc ctcaccaaac catgccccat ggacactctg
      181 gtgaccacca tccgcgcggg ggcggcgcag taccggctgg tcaccgcaga gcgcgagctc
      241 ctggagaaga cccttaccgg aagcatcaag gtgttgaccg acgtgctctc cctggcgaac
      301 ccggcggcgt tcggccgcgc ggctcgggtg cgtcggatcg tgtactccgt cgccgaggcg
      361 atgggactcg ccgatgcgtg gctctatggc atcgctgcca tgctctcgca agtgggatgt
      421 gtatccctcc ccgaggtcat tctccataag ctcaactccg gccagcccat ggatgcggag
      481 gaacagcggc tgttcgagga gcatccaggc atcggaagtg agctcctcgc caacatccct
      541 cgactggagc cggtggcaaa gatcgtcgcc taccaggaga agaactacga tggcaccggg
      601 gtcccggccg acgacgtcaa aggcgacgag atacctctgg gcgcccgtgt gctccacgcc
      661 gcgcttcatt acgacgcact cctgtcggcc ggcaagtcga gtacagccgc cgttgccgca
      721 atgcaacggc gctcagggca ctacgatcct gccatcgtct cggcactggc cgaagtggca
      781 ggggccgaga gctcatggga ggagcggctg gtgtcggcct gggatctcgc agcaggcatg
      841 tcgctcctcg atgacgtaac cacaccaacc ggcgccctcg tggtctgcag aggccaggag
      901 gtgaccccgt cgatccgagc ccgcctgatc agcctggtca ggcgagccgc tgtccgagag
      961 ccatttcggg tgctcgcgcc agtcaccccg atgaggggtg gcgatgtctg aggccaggtc
     1021 tgagccctgg ttgtccgacc tcgcggatcg ccctggcaca agagagcccc tcgggtcgga
     1081 gtgtctcgcc tccctgttga agggtgaatg gcgccccgtc ggcgagcgca ccgagttccc
     1141 cggcgtctcc atccgtcaca cagactcgag gcggccgccg ttactccccg tactgctgga
     1201 cgcgctgaag ctgctctcga tggcgtggga agtcctgccg cccaggcagc agcccgccga
     1261 cgagacgacc caggcggagg gctgactcga cggcgccgcc ctgctcgccg ccagtgccga
     1321 cgtcgccagg accgcgctgc ggcgcgcgag ccgcgatggg gggcgcggcc gcaggctatc
     1381 tccgccagcc ggcccgcgag gcccgagggg cccggtccgc cgcctctgct tgcgccacga
     1441 tctcgaacgg caacgcaggc aacactccgg ccgcaacccg tcgtgttgtt gccggcttgc
     1501 cgccctgggc cttggtctcg agttccgcgt acagctcgac gatcagattg tcgacgtacc
     1561 cggccggcgg ggagtcacta ccggcgctca gtgtcagcag caggccgccg ggcactggct
     1621 cgacaccgtc cagcgcgatc cccgccggcg gctcgctcag cgagagccga accatcgaca
     1681 gaagcggccc ggccggcgag ttcacccgca cctgcacact cccgcccgcc gggacccgca
     1741 ccggccccgc gtcagcccag gacaccgtcg gcacccgccc ccccaacccc gtcgtcgtcg
     1801 cgagcagctc catcgagggc accaggtgcc ggtacgcgaa ggcctgcatc atgtcttcag
     1861 ccggaacggc ttcgtgaatc accatctgcc cagcgatcgc cgcggtcccc tgcagccgca
     1921 gtgacacagg cctcccgaac ggctgcttcg gcgccgagac cgtgatgcgg atcgagtcga
     1981 gcccagccgg gacgcgccct cccgccagcg cgtagccggc tggggcatca gcgagcgaga
     2041 cctcgatctc accgtcgaag ccgtccttgc ggatggcgtg taccgtcgcc gcggccgacc
     2101 gccccgccgg aatgctcagg ctcgagggcg tcacccgcag tgcgaagtcg ggtcggggcc
     2161 cgctcagacg aagccggtag ccatagcgtt cgcccccatg ctgctgaaca tccgacacgc
     2221 ggacgaagta cgggccatct gccggtagct ccgccctcac atacgagtcg gcgtggtgcg
     2281 tgataagccc ggccccgggg tcggggctat cgtcgttcca cgcgatgatc ttgccggcgc
     2341 cgtccatgag ccgcaccagc gagtccagtt cggagccgag ccgacgagca ctcacctcgg
     2401 cgacgactgt ctcccccgcc cggccatcga agcggtacac atccacatcg cccgcgcgct
     2461 caatccggcc gctcacaccc acaggcagac ggacactctg cgcacgctcc aggccatcgt
     2521 tcggctcggc ctcaacgcac tcaggcag
//
LOCUS       DAOSPL010000626         2547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40715_length_2547_cov_2.387239, whole genome shotgun sequence.
ACCESSION   DAOSPL010000626 DAOSPL010000000
VERSION     DAOSPL010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40715_length_2547_cov_2.387239"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="PLQ54_11620"
     CDS             <1..1081
                     /locus_tag="PLQ54_11620"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOS93955.1"
                     /translation="RRQFVRTATALLGGCSAVLAAGSAGAQGEEAADRPASRRQGWRR
                     SPNYGRTDGRETVARAVAMAGTVADGPKVAVARCPRIWSDETGVDPQVLAEVIDHAMM
                     WFVGADSAEKAWRSLFGSDERIGLKPNGLGGIELSTSEALSAYVVDRLTGIGVKRENI
                     IAWEQNAGFIANCGIPVDEPPWGIQAVITGDALGQRVTHGTVDQRLTSVVTDRVDAIV
                     NLPILKDHAMSGVTLSMKNHYGTVEAPSALHGNFRDHLADLASVPAIRDKTRLILCDM
                     THCVVDGGPMGSPHFFPGAIMVASDMAAHDALGTAILEEERKERNMPSLAQVGKPPTF
                     LAAAQERGVGIADLAEVNAKLLEYA"
     gene            complement(1698..>2547)
                     /locus_tag="PLQ54_11625"
     CDS             complement(1698..>2547)
                     /locus_tag="PLQ54_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOS93956.1"
                     /translation="LSCEAATFDPHLRPRDLPHALTQFVDRLLRGLDRERVLGLGCAA
                     SGVVDPATGVLLLSGALLSDDDVPVVDFPLRDYLAELFPWPVHVANDANVGGLAVFKR
                     LVRQGQIPPDGSLFYAMAVDDLWGFGCGLVVRGVPYAGAHGAAGEIIHPRLTLWRPPP
                     EGLAAKALDGDAESMRAIMGYLVPVIEHLAALAVSLDPDRIVLGGAFATVGDPFRSEF
                     ERVLRTTLPFDSFLTGISRAGVLMDPLWPDTVAVGAAETVLDGLFEPQANGEPTALVR
                     MALEAG"
BASE COUNT          425 a          845 c          891 g          386 t
ORIGIN      
        1 ccgccgccag ttcgtccgga ccgccaccgc gctgctgggt ggatgctcgg ctgtcctcgc
       61 cgctggtagt gccggagccc agggcgagga ggcggccgat cggcccgcgt ccaggcgcca
      121 aggctggcgc cgtagcccga actacggccg cacggatggc cgagagacgg tggcgcgagc
      181 ggtcgccatg gccggcactg tggccgatgg gcccaaggtc gccgtggcgc gctgtccgcg
      241 gatctggagc gacgagacgg gcgtcgatcc ccaggtcctg gccgaggtca tcgaccacgc
      301 gatgatgtgg ttcgtcggcg ctgattcggc ggagaaggcc tggcgatcgc tgtttggctc
      361 cgatgagagg atcggcttga agccgaatgg cctcggtggc attgagttat ccacgtccga
      421 ggcgctgtcg gcgtatgtgg ttgaccgcct cacgggcatc ggagtgaagc gcgagaacat
      481 catcgcctgg gagcagaacg cggggttcat cgccaactgc ggcattcccg tggatgagcc
      541 accgtggggc atccaagccg tgatcaccgg cgatgcattg ggtcagcggg tcacccacgg
      601 gaccgtcgac caacgcctca ccagcgtcgt caccgaccgg gtcgacgcga tcgtgaacct
      661 gccgatcctc aaggaccacg ccatgtcggg tgtgactctc tcgatgaaga accactacgg
      721 cacggttgaa gccccctcgg cgctgcacgg caacttcaga gaccacctgg ctgacctggc
      781 ctcggttccc gccatccggg acaagacccg cctcatcctg tgcgacatga cccactgcgt
      841 ggtcgatggc ggccccatgg gctcgcccca cttcttcccc ggcgcgatca tggtggcgag
      901 cgacatggcc gcccacgatg ctctcggcac cgccatcctc gaggaggagc ggaaggagcg
      961 caacatgcca agcctcgccc aggtgggcaa gccgccgacc ttcctggcgg cggcgcagga
     1021 gcgtggggtc ggtatcgccg accttgcgga ggtgaacgcg aagcttctgg agtacgccta
     1081 gctcgggccc ccccttctcc gacgcctcgc ctccgggaga ggactcattg ggccggccag
     1141 cgggcactgt cgagctgcct tccgccccag gagaccatgc atacgcctag atcacgttta
     1201 atggcttgac tataatgcca taatatggta taatgcgtac atgtgtttgc aaggaaggcc
     1261 gtccggctgc ccggctgtcc ccctccacgg acctcgcgag gaccccgcga ccatgtctga
     1321 acccgcgcac gggctgcggg ccgcacctgc tccaccggtc tgcggcccgg gccaaggcca
     1381 gtggcgagca tgccacgagc cacacccgac tcgggtggga gctcaaggaa cgcgggtggg
     1441 tgatgatcca ctcctcttca tgcgtctccc ctggccgatg gtcccgggaa ggtctggtct
     1501 tgcgcggggt ttcgccacct gaagtgtgga gcgtggagcg ttgtggaggg ttcttgggta
     1561 ttttcggcga gaggccgcgt tcagaccgaa aatacccaga aacgctccac aaccctccac
     1621 accctccaca ccgggcgagg ggggtctttg ggagcgcccg cttcggccgg cgctcgcggc
     1681 gggggcgacg accgtcccta tccggcctcg agggccatgc ggaccagagc cgtgggctcg
     1741 ccgttggcct ggggctcgaa gaggccatcg agaacggtct cagcggcgcc gacggctacc
     1801 gtgtcgggcc agaggggatc catgagcacc ccggcacgcg agatgccggt taggaatgag
     1861 tcgaagggta gcgtcgtccg aaggacgcgc tcgaactcgg accggaacgg atcgccgacc
     1921 gtcgcgaagg ccccgccgag gacgatgcgg tccgggtcca gggacacggc cagtgcggcc
     1981 aagtgctcga tgacgggcac gaggtagccc atgatggctc gcatgctctc ggcatcgccg
     2041 tccagggctt tggcggccag tccttcgggc ggcgggcgcc acaaggtgag gcgtgggtgg
     2101 atgatctcgc cggcggcgcc gtgggcgccg gcatagggta cgcctcgcac gacgaggccg
     2161 cacccgaagc cccacagatc gtctacggcc atggcgtaga acagggagcc gtcgggcggg
     2221 atctgcccct ggcgcacgag ccgcttgaac acggccaggc cacccacgtt ggcgtcgttg
     2281 gccacatgga ccggccaggg aaagagctcg gccaggtagt cgcgaagtgg gaagtccacc
     2341 accggcacat cgtcgtccga cagcagtgct cccgagagga gcaggacgcc ggtcgccggg
     2401 tcgaccacgc ctgaggccgc gcagcccagc cccaggaccc gctcccggtc cagccctcgc
     2461 agcagccggt ccacgaactg ggtgagcgcg tgaggcagat cgcgtgggcg gaggtggggg
     2521 tcgaaggtcg ccgcctcgca ggacagg
//
LOCUS       DAOSPL010000627         2546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40738_length_2546_cov_2.695705, whole genome shotgun sequence.
ACCESSION   DAOSPL010000627 DAOSPL010000000
VERSION     DAOSPL010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40738_length_2546_cov_2.695705"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2380)
                     /locus_tag="PLQ54_11630"
     CDS             complement(<1..2380)
                     /locus_tag="PLQ54_11630"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOS93957.1"
                     /translation="MFVVNARRCLCECPDGETPAQRMGLEEQECPPPEAVEGLPRPVV
                     SAQRHPLQAQVPAREWGEGQVVGDEFTVLGHLGRGGMGTVYLVESRAGGHLYAVKRAL
                     AAGASDEALGRAFRRELQQWIGLPPHPHIAACRFVRTIDGQIAVFSEYVEGGSLADWI
                     RQGRLASAAVVLDVAIQVAWGIDAAHCHGTVHQDVKPGNVLMTSDGIAKVTDFGLARA
                     RRPLEAPETGSDTFRLAVARSQPSEASSPFVSFAGYTPAYCSPEQYARRPVSAATDVW
                     SWGVSVLEMVLGAVTWSLGPEAPMALERHLGALAIGGVAAGGRPDEARQGLRRVPDAL
                     VQVLGRCFRQDPAGRWSSLREAADALVAAYEVETGRRYPRTAPERAEPTRGDPSTDGV
                     LDGALYWLWRARAAAGLVPTTPGARAPASPRSARARAVDELSRYEEAVALYQRVVDRG
                     HTEFLDDLADACAAKAEAHQQLGDYEGALECYDMAARRLARHWRATREAAAALGLARC
                     YQRKAGFLHQVGENRAVLPLCEQAITLSEQEYQTSGSSEAAAILSSSLECQANALGDL
                     GAYDESLAAFDRALALLRRIHGEGDAGGFGALARVYGNKAITLRASGQLAAAVVCYDQ
                     AIGIRERLRDRGMLGAAAYDLAVNYMNKGAALGYSGDSVRAAECQGQAIEVLEELIST
                     EGPERSGFALSLCYSNRGVELEQLGRYDEAMACYDRAVDMLRRLAHGESRQRVMGEFG
                     RSLVHRAGLRLRRGQAEMARMDAREAESVLSAEAQRTGRPDMRESLEAARALL"
BASE COUNT          392 a          958 c          826 g          370 t
ORIGIN      
        1 gcaggagggc ccgcgccgcc tccagagact cccgcatgtc cggccgtccg gtccgctggg
       61 cctcggcgct cagtacagac tccgcctcgc gcgcatccat cctcgccatc tcagcctgcc
      121 cgcgccggag gcgaagcccc gcgcggtgaa cgagggagcg gccgaactcg cccatcacac
      181 gctgccggct ctcgccgtgt gcgagacgcc tcagcatgtc cacggcgcga tcgtagcacg
      241 ccatggcttc gtcgtatctc ccgagctgct ccagctcaac gcccctgttg gagtagcaca
      301 gcgagagggc gaacccgctc cgctccggcc cctctgtcga gatgagctcc tccagcactt
      361 cgatggcctg tccctggcat tcggccgccc tcacactgtc gcctgagtag cctagtgccg
      421 cgcccttgtt catgtagttg accgccaagt cgtacgccgc tgcaccgagc attccgcggt
      481 cccgcagccg ctcgcggatc ccgatcgctt ggtcatagca caccaccgcg gcggcgagct
      541 ggccgctcgc gcgcagggtg atggccttgt tgccatagac ccgcgcaagg gcgccgaacc
      601 cacccgcgtc gccctcacca tggatgcggc gcagcagcgc tagggcccgg tcgaaagctg
      661 caagcgactc gtcataggcc cccagatcgc caagggcgtt ggcctggcac tccagggacg
      721 atgacaagat ggctgcagcc tctgacgacc cgctggtctg gtactcctgc tccgacagcg
      781 taatggcctg ctcgcacaac gggagcactg cccggttctc gccgacctgg tggaggaacc
      841 cggccttgcg ttggtagcac cgggcaagcc caagcgcggc ggccgcctcg cgagtggccc
      901 gccagtgacg ggcgagccgg cgagccgcca tgtcgtagca ctccagggca ccctcatagt
      961 cgcccagctg ctggtgggcc tctgccttgg ccgcacaggc gtctgccagg tcgtcgagga
     1021 actccgtatg cccgcggtcg accacccgct ggtagagcgc cacggcctct tcgtacctgc
     1081 tcagctcatc gaccgctcgt gcccgggcgg accggggact ggccggagcc cgagcccccg
     1141 gggtcgtcgg aaccaagcct gccgcggccc tggcccgcca gagccagtag agggccccat
     1201 ccaggacgcc gtcggtgctc ggatcgcctc gtgtcggctc agcgcgttcg ggcgccgtcc
     1261 tcgggtagcg tcggccggtc tccacctcgt aggccgccac gagcgcgtcg gcggcttccc
     1321 gcaatgagga ccagcgtccg gctgggtcct gacggaagca ccggcccagc acctgcacca
     1381 gcgcgtcggg gacccgccgg agcccttgcc tggcctcgtc tggccggccg ccagcggcca
     1441 caccaccgat cgcgagagcc ccgaggtgcc gctccagcgc catcggcgcc tcggggccaa
     1501 gcgaccatgt gacggcgcca aggaccatct ccaggaccga cactccccag ctccacacgt
     1561 cggtggcggc agacaccggc cgccgcgcgt actgctcggg cgagcagtag gccggcgtgt
     1621 acccggcgaa gctgacgaac gggctgctgg cctccgaggg ctgggaccgc gcaactgcga
     1681 ggcgaaaggt gtcgctcccc gtttctgggg cctcgagggg gcgacgcgcc ctggccaggc
     1741 cgaagtcggt cactttggca atgccatcag acgtcatgag cacgttcccc ggcttgacgt
     1801 cctgatgcac cgtcccgtgg cagtgggcgg catcgatgcc ccacgcaacc tggatcgcca
     1861 catcgaggac gacagccgca ctggccagtc gcccttggcg aatccagtcg gctaggctgc
     1921 caccctccac gtactcggag aaaaccgcga tctggccatc gatggtccgg acgaaccgac
     1981 acgccgcgat gtgagggtgc ggcggtaggc cgatccactg ctggagctca cgacggaagg
     2041 cccggcccag tgcctcatcg ctcgcgcccg ccgccagcgc gcgcttcacg gcgtagagat
     2101 gaccgcctgc ccggctctcg acgagataca cggtccccat gccgccgcgc cctaggtggc
     2161 cgagcaccgt gaactcgtcg ccgactacct ggccctcgcc ccactcgcgc gccgggacct
     2221 gcgcctgcag cggatgcctc tgggccgaga ctaccggccg cgggaggccc tccaccgcct
     2281 ctggaggcgg gcactcctgc tcctctagcc ccatccgctg agcgggggtc tcgccatcgg
     2341 ggcactcgca caggcagcgc cgcgcattga caacaaacaa ccggcggcaa ccgcggcacc
     2401 gtgcgggttc gccaaccagc caagccaggt ggttggggac ggaatggagg gctccgcagt
     2461 gagggcaggt cgtcttggtg tagcagcagt agagctcgcc cagtcgcccc ctcagttcct
     2521 atggtcggac acgccgaacc tggaca
//
LOCUS       DAOSPL010000628         2545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40747_length_2545_cov_4.304016, whole genome shotgun sequence.
ACCESSION   DAOSPL010000628 DAOSPL010000000
VERSION     DAOSPL010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40747_length_2545_cov_4.304016"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PLQ54_11635"
     CDS             <1..367
                     /locus_tag="PLQ54_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93958.1"
                     /translation="REYIDHEGTGGELTITRSRTATGAGYVFRAQRALFVGDRRAELP
                     GLEFASRSGGPANVMLAWGTDSLRLMCDADVDVKLRPALFAGGLGVGARVSGRHGGMR
                     IVGPAIEIRALEGETVELR"
     gene            391..1605
                     /locus_tag="PLQ54_11640"
     CDS             391..1605
                     /locus_tag="PLQ54_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93959.1"
                     /translation="MRHRATWGLSAVVLLTVAMLPAGTEDGATIDVRSLGAIGDGQAD
                     DTDAFARALALGREQHKPVMVSVGQYRLTRAIRLEEQCLTGVACGGWPADSMPMPVLR
                     IDHTDSPGVVLDAHASLLDLAILYPEGTQFPEEGGPPAVSLEGQGPTISRVRIQYPYD
                     GIMTAPGAFPGRARLSDIFIVSPKRDGVYLTKSLDVSQLRNIEVWCNVGLSTGAGFRF
                     GRNDDCQCSDLFAFQCQVGFEFDPDEDPGPGGGTFYGSLTNCSTDACSLGYVVRGNHR
                     LNVANSDLVDHHASLDVDGEQASVRLTGCWIQTNGAPAIRVTRAANVSVANSLFTRAF
                     AADFPYVEAAACGSLIVTGCQFRPLSRGIELGEGVQRAVVVHNVFESDQPAIVDRMGP
                     DAKRILEPNVEG"
     gene            1610..>2545
                     /locus_tag="PLQ54_11645"
     CDS             1610..>2545
                     /locus_tag="PLQ54_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93960.1"
                     /translation="MRLAAVLFLVAAPTAAAPAQVPGLPACLHRWDFETGTQGWTKWS
                     DGEMVQECRQGGAHLGRSALWGSVTGRHALVHSVDGLSVPMSAVLRLWLYAPEGRAPD
                     RITLSLRTYEHGPQSSPMFQAAFGVRPGRWQLIEVPFSELFGWSDRVFDSWTIRQFEV
                     SADGPVEVALDDVEILAPPGRQPPRPSVMWLPGQDVTQAPAGARAQFSHTFELDHMPR
                     YAWLQAGGDDQGTAFVNGVELGTASLTGPGEYDLVPHLRVGRNMVCLAVANHGEAPNP
                     SGGLAVIGWGDARPDEHVVVSGTSWRSSAGAPNGWT"
BASE COUNT          363 a          862 c          897 g          423 t
ORIGIN      
        1 ccgagagtac atcgaccatg aaggcacggg cggcgagctc accatcactc ggtcgaggac
       61 ggccaccggt gcgggctatg tcttccgagc gcaacgggcc ctgttcgtgg gtgaccggcg
      121 cgccgagttg ccggggctcg agttcgcgtc gcgctcgggt gggccggcca atgtcatgct
      181 ggcgtggggc actgactcgc tccgcttgat gtgcgacgcg gacgtggacg tgaagctacg
      241 gcctgcgctg ttcgccgggg gcctgggggt gggagcgcgg gtcagcggcc ggcatggagg
      301 catgcgtatc gtcgggccgg cgatcgagat tcgcgccctc gagggagaga cggtcgaact
      361 gaggtaggca gcgccagagg aggcatctcc atgcgtcatc gggcgacctg gggcttgtcg
      421 gcggtcgtgc tgctcaccgt ggccatgctg cccgccggga ccgaggatgg cgcgaccatc
      481 gatgtgcggt cactgggcgc gatcggcgat gggcaggctg atgataccga tgccttcgcc
      541 cgagcactgg cgctcgggcg ggagcagcac aagcccgtga tggtgtctgt gggtcagtac
      601 cggctgaccc gggccatacg gctagaggag cagtgcctca ccggcgtcgc gtgtggcgga
      661 tggcccgctg acagcatgcc catgcccgtg ttgcgcatcg atcacaccga ctcgcccggg
      721 gttgtgctcg acgcccacgc ctcgctgctc gatctggcga tcctctaccc cgagggaacg
      781 cagtttccgg aagagggagg cccgcccgcc gtctcgctcg agggccaggg cccgacgatc
      841 agccgggtgc ggatccagta cccctacgat ggcatcatga cggccccggg cgcctttccg
      901 ggccgcgcgc ggctgtccga catcttcatt gtctcgccga agcgcgacgg ggtttaccta
      961 accaagagcc tcgatgtgag ccagcttcgg aacatcgagg tctggtgcaa tgtgggcctg
     1021 tcgaccgggg cgggcttccg cttcggccgg aacgatgact gccagtgttc cgacctgttc
     1081 gccttccagt gccaagtggg gttcgagttc gaccccgacg aggacccggg gccgggcggt
     1141 gggacgttct acggctcgct gacgaactgc tcgaccgacg cgtgctcgct ggggtacgtg
     1201 gtgcgcggca accaccggct caacgtggcg aactctgatc tggtcgacca ccacgcgtcg
     1261 ctcgacgtcg acggtgagca ggcaagcgtt cggctgacag gctgctggat ccagaccaac
     1321 ggtgcccccg cgatccgtgt gacgcgcgcg gccaatgtct ccgtcgcgaa ctccctgttc
     1381 acgcgcgcct tcgccgccga cttcccctac gtcgaggcgg cagcctgtgg gagtctgatc
     1441 gtcaccggct gccagttccg gcccctgagc cgcggcatcg agctgggcga gggcgtccag
     1501 cgcgctgtag tggtccacaa cgtgttcgag tccgaccagc cggccatcgt tgaccggatg
     1561 gggccggacg cgaagaggat ccttgagcct aacgtggagg gctgacgcca tgcgcctggc
     1621 cgctgtgctg ttcctcgttg ccgcgcccac ggccgcagcc ccggcccagg tccccggcct
     1681 tcccgcctgc ctgcaccggt gggacttcga gaccggcacc cagggctgga cgaagtggtc
     1741 cgatggtgag atggtccagg agtgccgcca gggcggcgca caccttggcc gctcggccct
     1801 gtggggcagt gtcactggcc gccacgcctt ggtgcactcc gtcgatggtc tgagcgtgcc
     1861 gatgtccgcg gtgctcaggc tgtggctcta cgcccccgag ggcagggcac cggaccgcat
     1921 cacgctcagt ctccggacgt acgagcacgg cccccagtcc agccccatgt tccaggcagc
     1981 cttcggcgta cgacccggtc gttggcagct catcgaggtc cccttctccg agctcttcgg
     2041 gtggtccgac cgcgtcttcg actcgtggac gatccgtcag ttcgaggtat ccgccgacgg
     2101 ccccgtcgag gtcgcgctcg acgatgtcga gatcctcgcc ccaccaggcc gacaaccgcc
     2161 ccggccgtcg gtgatgtggc tcccggggca ggacgtgact caggcgcccg ccggcgccag
     2221 agcgcagttc agccacacgt tcgagctcga ccacatgccg cgctatgcct ggctgcaggc
     2281 gggcggcgac gaccagggaa cggctttcgt caacggtgtc gagctgggca ccgcatcgct
     2341 caccggcccg ggcgagtacg acctggtgcc tcacctgcga gtcgggcgca acatggtctg
     2401 cctggccgtg gccaaccacg gcgaagcgcc gaatccctcg ggcggtctgg ccgtcatcgg
     2461 ctggggcgac gcccgacccg acgagcacgt cgttgtcagc ggtacgtcct ggcgcagttc
     2521 ggcgggcgcg ccgaacggct ggact
//
LOCUS       DAOSPL010000629         2543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40792_length_2543_cov_2.250000, whole genome shotgun sequence.
ACCESSION   DAOSPL010000629 DAOSPL010000000
VERSION     DAOSPL010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40792_length_2543_cov_2.250000"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PLQ54_11650"
     CDS             complement(<1..681)
                     /locus_tag="PLQ54_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93961.1"
                     /translation="MVTVRGNAIDVETRTLVARIENGLITRLRSKAGGTERIRGGPWD
                     YSALQLVYPGQGPLDVLDRLAGRVSCHQRSAGCAEVRLHGWDADGVIRVSEDEATGDL
                     VVEPSAYSSRAGVLSCRWLVPGIAADLELIAPFFQGVRLPLSDSLLEGRWFWPQLWEA
                     GLAILQGRGGGFWIHCQDSRYRYKALNIEKATLGLETEAYGPVERNRGAGGLQWRVNV
                     YEGEWRVPA"
     gene            complement(662..1225)
                     /locus_tag="PLQ54_11655"
     CDS             complement(662..1225)
                     /locus_tag="PLQ54_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93962.1"
                     /translation="MKHIVVVDFATASCILGAVKDVDAVVSIGDPGSAPPPTLHREGR
                     QILRLEFHDVERDSVTAEELIAAGIPSVGFTPPSREMVRELLDWSPRLLAGEGTLVCH
                     CLSGLSRSTACAAVLAAVDRGPGGELAAMATVASRTQHVFMPNRAILRLADELRGPGS
                     RLLEAYDTWRTSDGEWLQGASHGYRPG"
     gene            complement(1222..>2543)
                     /locus_tag="PLQ54_11660"
     CDS             complement(1222..>2543)
                     /locus_tag="PLQ54_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93963.1"
                     /translation="TLRRFLDTHPDCAFQGVNGGGMGLNWEYLGYASGFQFTDGQAGQ
                     LASYYATYLFPPDKINDMPDIWDPAKFDPATWRSLLCTNFDMTGDTFDLAQLEGIRQI
                     VDIYHYLQSRGVVGRWVRVYHPTVTGDDPTMYLQRLSWDRKRGIIITKHRITGVVVVH
                     PKGLEPYERYEVTLQESPESFVRTGAQLMEDGVRLSDPPPGELIYLNLSDHPGNRVDR
                     TPPATPGSVASSVAQHMGVPGVDLRWAPADDDCWLSYYEIARDGKIVGRVAKGCFYFD
                     HSADADPAAVYAVRAVDGAGNVSAWAAAKPCAGPRRQVVRAVGGQGLRFTGTWEPEAD
                     LDPIGNGTLLRAAAAGASATLSFTGRGLVIHSRLGERGGLARIAIDDEEPVVVSCYSA
                     DEIPQWPLFERGWDASSEHHVRIECLGQPDARGTGTNVWLDAVAVHP"
BASE COUNT          417 a          903 c          824 g          399 t
ORIGIN      
        1 cgcggggaca cgccactcgc cttcatagac attgactcgc cactgcagcc cgcctgcgcc
       61 ccgattgcgc tcgaccgggc cgtaagcctc ggtctcgagc ccgagtgtcg ccttctcaat
      121 gttgagcgcc ttgtagcggt agcgcgagtc ctggcagtgg atccagaatc cgccgccgcg
      181 cccctgcagg atcgccaggc cggcctccca caactgcggc cagaaccatc ggccctccag
      241 cagcgagtcg ctcagtggca ggcgcactcc ctggaagaag ggtgcgatca gctccagatc
      301 cgcggcgatg ccgggcacga gccatcggca tgagagcaca cccgcacgcg aggagtacgc
      361 cgagggctcg acgacgaggt cgcccgtcgc ctcgtcttcc gagacacgga tcaccccgtc
      421 cgcgtcccac ccgtggagac gcacctcggc gcaccctgcc gaccgctgat ggcatgagac
      481 ccgccccgcc agccggtcga gcacgtccag cgggccctgg cccgggtaca ccaactgcag
      541 agccgaatag tcccacgggc cgccccgaat gcgctcggtc ccgcccgcct tgctccgaag
      601 ccgcgtgatc agcccgttct cgatacgggc caccagtgtg cgtgtctcga catcgatcgc
      661 gttaccccgg acggtaacca tgcgacgccc cctgtagcca ttcgccgtcg gatgtacgcc
      721 acgtgtcgta cgcctcgagg agccgtgagc ctggcccccg cagctcatcc gcaagccgca
      781 ggatggcccg gttcggcata aaaacgtgct gggtgcggct cgccacggtc gccatggctg
      841 ccagctcgcc tcccggtccg cggtcgacgg cggcaagaac cgcggcgcag gcggtgctcc
      901 gcgacaggcc cgacaggcag tgacacacga gggtcccctc ccctgccagt agcctgggtg
      961 accagtcgag cagctcacgg accatctctc ggctcggcgg ggtgaagccg accgaaggaa
     1021 tacccgcggc aatcagttct tcggccgtca ccgagtcgcg ctccacatcg tggaactcga
     1081 ggcgcaggat ctggcggccc tcgcggtgga gcgtcggagg cggagcggag ccgggatcgc
     1141 cgatggacac caccgcatcg acatccttca cggcgccgag aatgcacgat gccgtcgcga
     1201 agtcgaccac gacaatgtgc ttcacgggtg caccgccacc gcgtcgagcc agacgttagt
     1261 gccggtaccg cgggcgtcgg gttgcccgag gcactcgatt cgcacgtggt gctcgctcga
     1321 ggcatcccac ccgcgctcga aaagcggcca ctgggggatc tcgtcggcgc tgtagcagct
     1381 gaccacgacg ggttcctcgt cgtcgatggc gattcgggcc agccctcccc gctctccgag
     1441 gcgcgagtgg atcaccaggc cgcgccccgt gaaggataga gtcgccgacg cgccggcagc
     1501 cgccgcgcgc aagagtgtcc cgttgccgat tgggtccaga tcggcctccg gctcccaggt
     1561 ccccgtgaag cgcaggccct ggccgcccac cgcgcgcacc acctggcggc gcgggcccgc
     1621 gcaaggcttc gcggcagccc atgccgagac attgcccgcc ccgtcaaccg ctcggacggc
     1681 gtagacggcc gccgggtcgg cgtcggcgga atggtcgaag tagaagcatc ccttcgcgac
     1741 gcggccgacg atcttcccgt cgcgggcgat ctcgtagtag ctgagccagc agtcatcgtc
     1801 cgcgggggcc cagcgcaggt caacgcccgg gacccccata tgctgcgcga cgctggaggc
     1861 cacgctcccg ggcgtcgccg gcggcgtcct gtcgacgcga ttccctgggt gatccgacag
     1921 gttcaggtaa atgagctcac cggggggcgg atcactcaga cggactccat cctccatgag
     1981 ctgggccccg gtcctcacga acgactcagg gctctcctgg agtgtgacct cataccgctc
     2041 gtagggctcg aggcctttcg ggtggaccac gaccactccc gtgatccggt gcttggtgat
     2101 gatgatgccc cgcttccggt cccagctcag acgctggagg tacatggtgg ggtcatcgcc
     2161 tgtcactgtg ggatggtaca cccgcaccca tctccccacg actccgcggc tctgcaggta
     2221 gtggtagatg tcgacgatct ggcggattcc ctccagttgc gcgaggtcaa acgtgtcgcc
     2281 ggtcatgtcg aaattggtgc acagaagcga tcgccatgtg gccggatcaa acttggcggg
     2341 atcccagatg tcgggcatgt cattgatctt gtcgggcggg aacaggtagg tcgcgtagta
     2401 gctcgccagc tgcccggctt gcccgtccgt gaactggaag ccgctggcat agccgaggta
     2461 ctcccaattg agccccatgc ctccgccgtt cacgccctgg aacgcgcagt cggggtgggt
     2521 gtcgaggaac cgacgcagag tct
//
LOCUS       DAOSPL010000630         2542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40800_length_2542_cov_3.774025, whole genome shotgun sequence.
ACCESSION   DAOSPL010000630 DAOSPL010000000
VERSION     DAOSPL010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40800_length_2542_cov_3.774025"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..956
                     /locus_tag="PLQ54_11665"
     CDS             54..956
                     /locus_tag="PLQ54_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS93964.1"
                     /translation="MVASYVRLAETGELESRAVRAREVLRSCQLCPHACGVDRLSGQV
                     GRCRTGARAIVSSYGPHFGEESVLVGRFGSGTIFFAHCNLACVFCQNADISHGGEGSE
                     VGPESLGGMMMALARRGCHHINLVTPTHVLPQILEAVAWAAARGLNLPLVYNCGGYEA
                     VDALRLLDGVVDIYMPDAKYADAEVGLRLSGARDYPVHLRAALAEMHRQVGDLVVGED
                     GVAERGLLVRHLVLPNGLSGADSVFRFLAGEVSRDTFVNVMGQYRPCHEAHRHAEIDR
                     RPTAEELRGARDAARAVGLWRLDS"
     gene            983..1570
                     /locus_tag="PLQ54_11670"
     CDS             983..1570
                     /locus_tag="PLQ54_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOS93965.1"
                     /translation="MRCSNCGGDVSDGVTICPYCLEALPGQTKPPTPPSSARSPEPIP
                     EACTNACPLCAQPRRPWANFCTGCGAPLGPRQVAYTVVVDWTEADHRRRQRALARALT
                     GIPAVVAARVSRAGPGPVGVVMPRTLAEQLAARMAYAGVAARVTPAEEAHRQFPQAFV
                     WGVQALPSGNAAPARNAARSPGCLLAGMALLGWGR"
     gene            1610..>2542
                     /locus_tag="PLQ54_11675"
     CDS             1610..>2542
                     /locus_tag="PLQ54_11675"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOS93966.1"
                     /translation="MPVDDGRDRVLDDIRALTGLGCVVFDDEGRVFGPTDGVQAVATA
                     DLLAPAARRAVAAGAAVVCRWPALGLACGAAPFGGSDGERAAVVVGPARERGVRARAV
                     ARVAREHGATPEALRERLRALPSAGETALLAAAQLAASALSGVAAAEACVAADPFDGP
                     LARSLGLLQERILEVGTDGRILRCTPAGYDSGNDVEDLIGRHVTEFIEAGMRNQAERD
                     LMRAWETGSALSVVRGVGRGGTPKHLAIAISRRDRPAGPATSVIGVVRDMTALHEARR
                     ASDRRTAQARLMADVAHALADPESLEWLMEEVTRV"
BASE COUNT          340 a          863 c          942 g          397 t
ORIGIN      
        1 tgtcctggat ccctgtgcgc accccggcga tctcggagag gagtacacgc ccgttggtgg
       61 catcgtatgt tcggcttgcc gaaaccgggg aactggagtc gcgcgccgtc cgggcacgcg
      121 aggtactgcg gtcgtgccag ctgtgccccc acgcctgcgg cgtcgatcgg ctctcgggcc
      181 aggtcggccg gtgccgaacg ggtgcccggg ccatcgtctc gagctacgga ccccacttcg
      241 gcgaggagag cgtcttggtt gggcgcttcg gctcaggcac catcttcttc gcccactgca
      301 acctggcatg cgtgttctgc cagaacgccg acatcagcca cgggggcgag ggttccgagg
      361 tggggccaga gagcttgggc ggcatgatga tggcactggc ccggcggggt tgccaccaca
      421 tcaacctggt gacgccgacg cacgtgctgc cccagatcct cgaggccgtc gcttgggcgg
      481 cagccagagg gttgaacctg cccctggtgt acaactgcgg cggctatgag gccgtggacg
      541 ccctgcgact actggacggc gtggtcgaca tctacatgcc cgacgccaaa tacgccgacg
      601 cggaggtagg tctgcgactg tcgggcgccc gcgattaccc cgtccacctg cgcgcagcgc
      661 tcgccgagat gcaccgccag gtgggcgacc tggtggtcgg ggaagacggg gtggccgagc
      721 gcggcctgct cgtccgacac ctggtgctgc ccaatggtct gtccggggca gacagcgtgt
      781 tccgctttct ggcgggcgag gtgtcgcgcg acacgttcgt gaacgtcatg gggcagtacc
      841 ggccatgcca cgaggctcac cgccacgcgg agatcgatcg gcgtccaacg gccgaggagc
      901 tacggggtgc tcgtgatgcc gcgcgggccg tggggctctg gcggctggac tcgtgagtcg
      961 tggggccgag gtgaggtgcg cgatgcggtg ttcgaactgc ggcggcgacg tctctgacgg
     1021 agtcaccatc tgcccgtact gcctggaggc ccttccgggc caaaccaagc cgcccactcc
     1081 accatcgtcg gcccgatcgc cggagccgat ccccgaggct tgcaccaacg cctgcccgct
     1141 gtgtgcgcag ccacggcgcc catgggccaa cttctgcacc ggatgcggtg cgccgctcgg
     1201 acctcgccag gtcgcctaca ccgtggtggt agactggacg gaggccgatc atcggcggcg
     1261 ccagcgggcg ctcgctcggg ccctcacggg catacccgcc gtcgttgccg cacgggtctc
     1321 ccgggccggt ccgggcccag tgggcgtggt catgccccgc acactggccg agcagctggc
     1381 ggcccggatg gcctacgcgg gcgtggccgc gcgagtgacg ccagccgaag aggcccatcg
     1441 gcagttcccc caggccttcg tgtggggcgt gcaggctctg ccttcgggga acgcggcacc
     1501 ggctcggaac gctgcccgat ctccgggatg cctcctggcg ggcatggcgc tcctgggatg
     1561 gggccggtag acggacacgc gccggacacc ggtgatgggg gtgcgcacga tgcctgtcga
     1621 cgatggtcga gaccgcgtcc tcgatgatat ccgggctctg accggcctcg gttgtgtcgt
     1681 gtttgacgac gaaggacgcg tcttcggccc cacggacgga gttcaggcgg tcgccacggc
     1741 cgatctgctg gctccggccg cgcgccgtgc ggtggcggca ggagccgccg tggtgtgccg
     1801 ctggccggca ctgggcttgg cgtgcggtgc ggcacccttt ggcggctcgg acggcgaacg
     1861 cgccgccgtc gtggtcggcc ccgcccggga gcggggcgtg cgtgcccggg cggtggcccg
     1921 ggttgcccgc gagcacggtg ccacgcccga agcgctgcgc gagagactgc gcgcgctccc
     1981 gagcgccggt gagacggcct tgctggccgc ggcccagctg gcggccagcg ccctgtccgg
     2041 tgtggcggcc gcggaggcat gcgttgccgc cgacccattt gatggacccc tggcccgatc
     2101 cctcggcctg ctccaggagc gtatcctcga ggtggggacc gatggtcgaa tcctccgctg
     2161 cacgcccgct ggctacgaca gtggtaacga cgtcgaggat ctgatcggac ggcatgtgac
     2221 ggagttcatc gaagccggca tgcgcaacca ggccgagcgt gacctcatgc gggcatggga
     2281 gacaggctct gctctgtccg tggtccgcgg ggtcggccgt ggcggcacac ccaaacacct
     2341 ggccatcgcc atctcgcggc gtgaccgtcc ggccgggccg gccacgtcgg tcatcggtgt
     2401 cgtgcgcgac atgacggccc tccacgaggc ccgcagggcg tcggatcgcc gcacagctca
     2461 ggccaggctg atggccgacg tggcgcacgc cctggcggat ccggagagcc tggaatggct
     2521 catggaggag gtcacccggg tg
//
LOCUS       DAOSPL010000631         2539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_40869_length_2539_cov_2.760870, whole genome shotgun sequence.
ACCESSION   DAOSPL010000631 DAOSPL010000000
VERSION     DAOSPL010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40869_length_2539_cov_2.760870"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..>2539)
                     /locus_tag="PLQ54_11680"
     CDS             complement(225..>2539)
                     /locus_tag="PLQ54_11680"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOS93967.1"
                     /translation="PGPVGVLSMCAFTRICLGLLLSAAMGGPAAHSHPLPAPAIRAFD
                     MHQVRLEPGLFRVAQEATRRYLHELDPERLLYAFRVNAGLPAPGEPLGGWERPDCEVR
                     GHFVGHYLSACGLMYASTGDEALRDRALYMVGELAKCQKALGGQYLSAYPESFFDRLE
                     SMADVPWAAYYTIHKIMAGLLDAYQYCHSEQALDVLLGMARYFGARTGRLPIYQMDRV
                     LAVEFGGMSEVLHNLYAVTGDPEHLELAHRFDRAEFLGPLALGHDNLSRIHANTHIPE
                     ICGAARRYEVTGEPAYREIVTFFWDRVVNTRTYATGGCSSGEMWPDPGCLAGTLGPNT
                     QECCTTHNMLKVTRYLYRWTGDPRYAEFTERALLNGILGTNRADTGQMIYYVPLSTGH
                     TKAFGTPNDSFWCCYGTGIESFSKLNDSIYFHDDHGLYVAQFIASTLDWHARGLRVEQ
                     RTRFPEEESTELRFRTDRRQRFTLHVRVPRWARGGVSIAVNGGRIQASAEPGSYLSLE
                     REWADGDRVRVRMPMALHVETLPDDPETGAILYGPVVLAGLTSRATYFVANPDDVAAW
                     VHPVAGDAPLVFSADAPNGQADERLNLIPWYRVVDEPYGVYWTMTREGSPTHRRILDE
                     ERAERQRQARVVDTIAIGDEANEREHNLQGEATASGSHLGRAWRHAAPGGWWSWDLAV
                     PADEPAVLCCDYWGSESGRRTFDILIDGTTLATQTLLNDAPGRFFSVEYPIPVEATRG
                     KDRVTVRIVPHEGNIAGGLFGCAVLRAAKP"
BASE COUNT          406 a          870 c          866 g          397 t
ORIGIN      
        1 ggtcaggggc cccgcgccct ctgggcgcac ccctgtgcgg cccctccgcc agaccaggcg
       61 agcgttccgc gcgaccttcg cgagacccag ccgcccggcc aagggcccta tgttaccgcc
      121 aatgggggca acggatcccc cagcctctgc gggagtgggt cccgcaccat tttcagaacg
      181 ctctgcccgg cgcgcggggt ccctggctgt tcctttggcg cgactcaggg cttcgcggcc
      241 cgcagcacgg cgcagccaaa caggcctccg gcgatgttcc cttcgtgagg gacgatccga
      301 accgtgaccc ggtccttgcc gcgtgtggcc tcgacgggaa tggggtactc gacgctgaag
      361 aaacggcccg gggcgtcgtt gagcagcgtc tgggttgcca gcgtcgttcc gtcgatcagg
      421 atgtcgaagg tccgccggcc gctctcgctt ccccagtagt cgcagcagag cacggctggc
      481 tcgtcggcgg ggaccgccag atcccagctc caccatccgc ccggcgcggc gtggcgccac
      541 gcccggccta ggtgagagcc cgacgcggtg gcctctccct gaaggttgtg ctcccgctcg
      601 ttcgcctcat cgccgatggc gatggtgtcg accacgcgag cttgtcgctg ccgctccgcc
      661 cgctcctcgt ccagaatgcg ccggtgggtg gggcttccct cccgagtcat ggtccagtag
      721 acgccgtatg gctcgtcgac cacccggtac caggggatca ggttaagccg ctcatccgcc
      781 tggccgttgg gtgcgtcggc ggagaacacc agaggagcgt cgccggccac ggggtggacc
      841 caggcggcca catcgtctgg gttggccacg aagtaggtcg cgcggctcgt gagcccggcg
      901 agcaccaccg ggccgtacag gatcgctccg gtctccgggt cgtccggcag cgtctcgaca
      961 tgaagtgcca tgggcatccg aacacgcacg cggtcgccgt cggcccattc gcgctcgagc
     1021 gagagatagg agcccggctc ggccgaggcc tgaatgcgcc cgccgttcac ggcgatactc
     1081 acaccaccgc gcgcccaccg cggcacccgg acgtgcagcg tgaagcgctg ccggcggtcc
     1141 gttcggaacc gcagctccgt actctcctct tcggggaaac gcgtccgctg ttccacccgc
     1201 agacctcggg cgtgccagtc gagagtcgac gcgatgaact gggctacata gagcccatgg
     1261 tcgtcgtgga aatagatgct gtcgttgagc ttcgagaacg actcgatccc agtgccatag
     1321 cagcaccaga aggagtcatt gggtgtgccg aacgccttgg tgtggccggt ggacagaggc
     1381 acatagtaga tcatctgacc cgtgtcggcc cggttcgtgc ccagaatgcc gttgagcaga
     1441 gcgcgctccg tgaactccgc atagcgggga tcgccggtcc atcggtacag gtagcgcgtg
     1501 accttgagca tgttatgtgt ggtgcagcac tcctgcgtgt tcggcccgag cgtcccggca
     1561 aggcaccccg ggtcgggcca catctccccg ctcgagcacc cccccgtggc ataggtgcgc
     1621 gtgtttacga cgcggtccca gaagaaggtc acgatctccc ggtaggcggg ctcgccggtc
     1681 acctcgtacc ggcgggccgc cccgcagatc tcgggaatgt gcgtgttggc gtggatccgg
     1741 ctcagattgt catgccccag ggccaggggg cccaggaact ccgcgcggtc gaagcgatgg
     1801 gccagctcca ggtgctccgg gtcgcccgtc accgcgtaga ggttgtgcag aacctcggac
     1861 atgcccccga actcgacggc caacacccgg tccatctggt agatggggag ccggccggtg
     1921 cgggcaccaa agtagcgcgc catgcccagg agcacgtcca gcgcctgttc ggaatggcag
     1981 tactggtacg catccagcag gccggccatg atcttgtgga tggtgtagta ggcggcccac
     2041 gggacgtctg ccatggactc caggcggtcg aagaaggact cgggataggc cgagaggtac
     2101 tggccgccga gcgccttctg gcacttcgcc agctcgccca ccatgtagag ggcccggtcc
     2161 cggagcgcct cgtcgccggt gctcgcatac atgaggccac acgccgagag atagtgcccc
     2221 acgaagtggc cccgcacctc acagtccggc cgctcccatc cacccagagg ctctcccggg
     2281 gccggcaagc ccgcgttcac gcggaaggcg tacagcaggc gctcggggtc cagctcgtgc
     2341 agatacctcc gggtggcctc ctgcgccacg cggaagaggc caggttcgag ccggacctgg
     2401 tgcatgtcga aggcgcggat cgcgggagcg gggagggggt ggctgtgagc ggccgggccc
     2461 cccatggccg ccgagagaag gaggcccaag cagatccggg tgaaggcaca catggacaag
     2521 acccccactg gcccaggcg
//
LOCUS       DAOSPL010000632         2532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_41031_length_2532_cov_3.007671, whole genome shotgun sequence.
ACCESSION   DAOSPL010000632 DAOSPL010000000
VERSION     DAOSPL010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41031_length_2532_cov_3.007671"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="PLQ54_11685"
     CDS             <1..1061
                     /locus_tag="PLQ54_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93968.1"
                     /translation="DVSWCFRFLTNELRRHERICGYVYTELQDIEWERNGFLNYDRTP
                     KSFGYDPTDINADDVLVLDAPPGQVVLPGAEIRVPAVASLFSPDRWAAARLSWQVDFT
                     DTLGRTRIAATEGAMDVSAPPWRVTGLGEMAVRLPAEPGLALVRAVLEDAEGHLLGRA
                     CLAFDVEGSAPPAGAVVVPFRPDGAAMDGLDGSVSGSSSAELVAGAGRGAITLSACVP
                     EGIPPAGLRRITFVAEMASYRPTDEQTDERTFPSDVEVRLGSVLIGKLTLPDCPADAN
                     GVLSYIHGIPGRYGYRVELKVYGPRLREVLRELRLGEAKVRVRIVGDDRDANGIAVLF
                     RGAGRFPMDPALIFETED"
     gene            complement(1108..2123)
                     /gene="pta"
                     /locus_tag="PLQ54_11690"
                     /pseudo
     CDS             complement(1108..2123)
                     /gene="pta"
                     /locus_tag="PLQ54_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008515.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
BASE COUNT          346 a          837 c          945 g          404 t
ORIGIN      
        1 gcgatgtctc gtggtgtttc cgtttcctga ccaacgaact gaggcggcac gagcggatct
       61 gcgggtatgt gtacaccgag ctccaggaca tcgagtggga gcgcaacggc ttcctgaact
      121 acgaccggac gcccaagtcg tttggctacg accccacgga catcaatgcc gacgacgtgt
      181 tggtgctgga tgcgccgccg gggcaggtgg tcctcccggg cgccgagatc cgagttccgg
      241 cggtggcgag cttgttctcg ccggatcgct gggcggccgc gcgcctttcg tggcaggtgg
      301 acttcaccga tacgctcggg cgaacgcgga tcgcggccac cgagggggcg atggatgtct
      361 cggcgccccc gtggcgcgtg accgggctgg gcgagatggc ggtgcgcttg ccggccgagc
      421 ccggactggc gctggtccgc gcggtgctcg aggatgccga ggggcacttg ttgggacggg
      481 catgcctggc attcgacgtc gagggcagcg cgccgccggc gggggccgtg gtggtgccgt
      541 tccgccccga tggcgcggcc atggacgggc tcgatggttc ggtctcgggg tcctcgtctg
      601 ccgagctggt ggcgggcgcc ggacggggag cgatcacgct gtcggcttgc gtccctgagg
      661 gcatccctcc ggcgggtctg aggcggatca ccttcgtggc cgagatggcg tcgtaccggc
      721 cgaccgacga gcagaccgac gaacggacat tcccgagtga tgtcgaggtg cgcctcggca
      781 gcgtgctgat cgggaagctg acgctgcccg actgcccggc ggacgccaac ggagtgctgt
      841 cgtacatcca tggcatcccg gggcgctacg gctaccgggt ggagctgaag gtctacggtc
      901 cgaggctgcg ggaggtgctc cgcgagctcc ggttgggcga ggcgaaggtg cgcgtgcgga
      961 tcgtggggga cgatcgcgac gccaacggga tcgccgtgtt gttccgcggc gccgggcgct
     1021 tcccgatgga tccggcgctg atcttcgaga cggaggactg acggggccgg gcgtggcgcg
     1081 gcccggcccc ggatcgtccc cgggcggcta cgccgccggc tgggccagga agttggcccg
     1141 aagcgcggca caggctgcca cgccaaggat gtcgtcggcg tcgcacccgc gcgacagatc
     1201 catccccgct ttggccaggc cctggacgag cggcccatag gcctggccgc ccgtcaggcg
     1261 ctccgagagc ttgtaagcga tgttccccgc atcgaggtcg gggaacacga gcacattggc
     1321 gctcccggcc acggggctgc cctggcactt gcgggcggcg acggagggga ccagggccgc
     1381 atcgccctgg agttcgccat cgacgaggat atcggggcgg cgctgccgca ccaggtcggt
     1441 ggcctggcgg accttgtcga tatcggcatg ctcggcactg cccttggtcg agaaggacag
     1501 catggccacc ttgggctcgg cctcgaggta gatgcgggcg ctttcggccg ccgagatggc
     1561 gatgtcggcc agctgctcgg ccgtgggttg gatcaccacg ccggcgtcgg cgtagatgag
     1621 ggcgccacgg accccgaact cctccctggg catgtccatg atgaagcagc tggagacggt
     1681 cttgaggccc ggcgccgggc cgcagacctg gagcagcggg cgaagccaat cgccggtggt
     1741 ggcgatgctc cccgccacgg caccatcggc gtcgccggcc tccatcatca gcgcggcgta
     1801 gtacaggtga tccctggata gctcgtcggc cgctgttccg tcatgccctt gtgcttgcgg
     1861 cgctcgagca gcaaggccgc gtaggaatcg tgccgcggcg acgtggctgg gtccacgatg
     1921 tgggcaccgt ccacgctcac gccggcgccg cgggcgtccc gggcgatggt gtccgggttg
     1981 ccgagcagga ccacggaggc cagcttctcc cgcagtacca gctcagcggc ctggagcacc
     2041 cgagtgtctt gtgtctcggg cagcacgagg gttttctgga gggctcgagc ccggtcgatc
     2101 aggctctgca tcacccatag catgttacac acgacctctc acgggttcct gatgggtcgg
     2161 tccgcagcct cgcgatccgg ccgcgggcga gctccacgcg cccaggggat acgcccctcg
     2221 gggtctcacg tccttcccct gggcgggcaa tcgagcacgg gcctggcccc ataggcgctc
     2281 acatacggct ctgggtcggg cgactctgtc tcgcgaaggt cgcgccggcc gccctgcctg
     2341 gtcttgcgga ggggcctgat agggggaacc agccgaaggc tggtgggacc ctaatcggcc
     2401 cggcgctcga cggccctcga cgggccagag agcgcatcca ggcggcgcga tcggcctccg
     2461 gtcgacccgg gcccattggg ggcccaccat ccggcttcgc cggacggtcc tccccatgtg
     2521 gcccctccgc aa
//
LOCUS       DAOSPL010000633         2531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_41046_length_2531_cov_3.399838, whole genome shotgun sequence.
ACCESSION   DAOSPL010000633 DAOSPL010000000
VERSION     DAOSPL010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41046_length_2531_cov_3.399838"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="PLQ54_11695"
     CDS             <1..1224
                     /locus_tag="PLQ54_11695"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOS93969.1"
                     /translation="LEYAVRQANELDLPLVVCFGLTDRYPEANARHYAFMLEGLAETR
                     TALERRGATFVLRRGNPSDIAIDVARSAALLVADRGYLRHHREWRQRVAQEVACPVIQ
                     VEADAVVPVESASPKAEYSAATLRPRLHRRLAEFLVPLAETPLRRRSPPATAAPPQLG
                     ELDVEAVPPVPRFHEGGTHRARRLLNQFIERRLRDYADRRNDPGGTCYSDLSPYIHFG
                     QISPLEIALRVVEAAGRPAADAFLEQLIVRRELSINHVYYTEGYDHYESIPVWARQTL
                     AAHTRDARGHTYGPGELEAGQTHDPYWNAAMREMVITGKMHPYMRMYWAKKILEWSLS
                     PEAAFEIALALNNRYFLDGRDPNSFAGVAWCFGTHDRPWPERPVFGTVRTMTLGGLER
                     KFDMAGYLKRVADLA"
     gene            1415..1621
                     /locus_tag="PLQ54_11700"
     CDS             1415..1621
                     /locus_tag="PLQ54_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93970.1"
                     /translation="MRARTKTRLWAGLIAGAIAVGICLAGLGCKRSGGANAGPTDEEK
                     AIAKQQKMKMEEKFRGITGKTPGQ"
     gene            complement(1923..>2531)
                     /locus_tag="PLQ54_11705"
     CDS             complement(1923..>2531)
                     /locus_tag="PLQ54_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOS93971.1"
                     /translation="LGVKPACAIIAGPDRAHYYPEAKTIIVKLIADSVGRKLLGAQIL
                     GPGDAVKRIDVAATALTAGMGIDDIACLDLAYAPPYAPAMDPLITAADALRNALDGLV
                     SYVFAAELEAMRQSGDGPFILDVRTPAEYQAAHIPGATLIPLGALRGRLREVPRDRPV
                     VALCKTSLRAYEAARILSARGDSNISVLAGGMAAWPYDTEAG"
BASE COUNT          425 a          851 c          866 g          389 t
ORIGIN      
        1 ctggagtatg cggtccgcca ggcgaacgag ctggatctgc ctctggtggt gtgcttcggc
       61 ctgacggatc gctatcccga agccaatgcg cggcactatg cgttcatgct cgagggcctg
      121 gccgagacgc gaaccgctct cgaacggcga ggagccactt tcgtcttgcg ccgggggaac
      181 ccgtccgaca tcgccatcga cgtggcccgc agtgcggcgt tgctggtggc tgaccggggg
      241 tatctgcggc atcatcgcga gtggcgccag cgagtcgcac aagaggttgc gtgcccggtg
      301 atccaggtcg aggccgacgc cgtggtcccg gtcgagtcgg cgtcgcccaa ggccgagtac
      361 tccgccgcca ccctgcggcc cagactgcac cgcaggcttg ccgagttcct cgtcccgctg
      421 gccgagacgc ctctgcgccg ccggagcccg ccggcaaccg ccgctccgcc gcagctcggc
      481 gaactcgacg tggaggcggt ccccccggtc ccgcgtttcc atgagggcgg cacccaccgc
      541 gcccgccgcc tgctcaacca gttcatcgag cggcgcctcc gcgattacgc cgaccggcgc
      601 aacgatccgg gcggcacctg ctactcggac ctgagcccct acatccactt cggacagatc
      661 tcgccgctcg aaatcgctct cagggtggtc gaggcggcag ggagaccggc ggccgatgcg
      721 tttctggagc agcttatcgt ccgccgtgag ctcagcatca accacgtcta ctacaccgag
      781 ggctacgacc actatgagag catcccggta tgggcccgcc agacgctggc cgcccacacc
      841 cgcgacgcgc gcgggcacac ctacggcccg ggagagctcg aggccggtca gacccacgac
      901 ccttactgga acgccgccat gcgtgagatg gtcatcactg gcaagatgca cccctacatg
      961 cgcatgtact gggccaagaa gatcctggaa tggtcgctgt cgcccgaggc ggctttcgag
     1021 atagcgttag ctctcaacaa ccggtatttc ctcgatggac gcgatccgaa ctccttcgcc
     1081 ggggtagcct ggtgcttcgg cacccacgat cgcccctggc cggagcggcc tgtgttcggc
     1141 acggtacgca cgatgaccct cggcgggctc gagcgcaagt tcgacatggc gggctacctg
     1201 aagcgcgtgg cagacttggc ctgacgtcag ggctcgcacg acggggccgg tcggcaacgc
     1261 ggccctcgga acggcccagc cctgtgcgga ctcggccaga catcggggag gtgtcgggac
     1321 cggcccgccg aaatccccca ctcaccacca ttgccgtgct cggtctgcgt gggccgagcc
     1381 ggagagagag cgtgtctggg agatgatgac ccgaatgcgt gcgcgaacca agacccggtt
     1441 gtgggcaggc ctcatcgcgg gggccatcgc cgtcggcatc tgcctggccg gcttgggctg
     1501 caaacgaagc ggcggagcca atgcgggacc caccgatgag gagaaggcca tcgccaagca
     1561 gcagaagatg aagatggagg agaagttccg cggaatcacg ggcaagacgc cagggcagta
     1621 gcgcaaccga gggcacggtt gcgtgttcca cggcacgcgc ggcacgttgg gcacccaacg
     1681 cccgaggctc gtcacccgga gcgctggagg gcggaacagc gggtctcggg cgtggcccca
     1741 ccacgcacgc gtgggacact caggtggaga cgccgaaccg ggtcagggct caccacatgg
     1801 aggctacgtc gccctcaatc acacgaacaa cgacttgtca cgagtacccg tctcattcca
     1861 tcggtttgcc tctcggctgt gtctgttctg gcggtgtgcc ctggtcacgg ccaggcgccc
     1921 cgctagcctg cttcggtgtc gtagggccag gcggccatgc cgccggcgag gacggagatg
     1981 ttcgagtcgc ctcgggcgga caggattcgc gccgcctcgt aggcgcgaag acttgtcttg
     2041 catagggcca ccacggggcg gtcacgcggc acctcgcgca agcgaccgcg aagagcgccc
     2101 agagggatca gggtcgcccc cgggatgtgc gccgcctggt actccgccgg cgtgcgcaca
     2161 tcgagaatga aggggccatc tccgctctga cgcatggcct cgagctcggc ggcgaagaca
     2221 tagctgacga gtccgtcgag tgcgttgcgc agcgcgtcag cggcggtgat cagagggtcc
     2281 atggccggcg cgtacggcgg ggcgtatgcc aggtccaggc aggcgatatc gtcgattccc
     2341 atacccgcgg tcagggcggt cgccgcgaca tcgatccgct tgacggcatc gcccggaccc
     2401 agaatctggg cgccgagcag cttgcggccg acgctgtcgg cgatgagttt cacgatgatg
     2461 gtcttggcct ccgggtagta gtgagcacgg tcgggcccgg cgatgatggc gcacgcgggc
     2521 ttcacgccaa g
//
LOCUS       DAOSPL010000634         2530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_41073_length_2530_cov_3.146263, whole genome shotgun sequence.
ACCESSION   DAOSPL010000634 DAOSPL010000000
VERSION     DAOSPL010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41073_length_2530_cov_3.146263"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="PLQ54_11710"
     CDS             complement(<1..1131)
                     /locus_tag="PLQ54_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93972.1"
                     /translation="MVNIMLTGFLVVALVTLCAPLGARSILEDDFGGSGVDASKWDVH
                     ASETPGSFVRVENGVLHLLGIGGGCTLTSLAEVPPFHVVEFDYLQPAAERLGGYANCV
                     SCQVTQSAPEQEWDGAAWYVEYSGGAWSHYGGRWDCRRSGARLAPEDDRWYHVTVFCQ
                     DTTAVIIVAERDTGRICWRAAFPHDPMVRGRIRFDAAAVGPDSRWGFMLDNISIRARE
                     AELEPACRPLGGRDTAFRIADAWRPIEIAGRTFWHLAEYRPDEWHEWGMADHPVFADA
                     AWLFAPNDRGLMAGERRPFTLEPSLDPETQRVQRRIVASARDRFLGFYGLKLGPGGPL
                     NEWGDGLIRMDMRDWFGGPIRDRYEGLERLRQRYEELADTPPV"
     gene            complement(1151..>2530)
                     /locus_tag="PLQ54_11715"
     CDS             complement(1151..>2530)
                     /locus_tag="PLQ54_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93973.1"
                     /translation="VVSPVRLVVADDEGVETLATVMEPSPDAPTCGRRAVETPYAGVT
                     LRRVGAGYACYFAFDVAQTMWAIHQGRPIDRDYDGDGYLRAMDARIVGDNDVAVPYTD
                     VLQSLLRVILGLRPIPMVDALPPAGGRVADAVFYFGGDDEGEAGNQVPASDFMRSRGL
                     PYHINLMPVGEGFAVSAQEAAHIEANGHELALHYNFVDGFSHPCGFTEHDVRAQADQF
                     MRAFGRSSRCSVNHWCRWCGWADPARWIAATGGTGDNSWFGPEPVGMNPTNVTGFSFG
                     TAFPRHVWDDWRHGNRRLPFVQEAITGYEVGYQDEQCDPAAVRRAVDLALAGRLTMCL
                     FYHPVYIAGSPGCREAIDELLRYVDELGATVAYMGSDRLNEWWMNRDATTVEDALFDG
                     NLLEFVVDCPDPSGCVIRVCLGQSVGARCFVESADVIPKRVTECASNWAYFPVPIGRH
                     RVAVQFLTA"
BASE COUNT          453 a          866 c          823 g          388 t
ORIGIN      
        1 caccggtggc gtgtcggcca gctcctcgta cctctgtctc aggcgttcga gtccctcata
       61 gcggtcgcgg attgggccac cgaaccagtc gcgcatatcc atgcgtataa gcccgtcgcc
      121 ccactcgttg agtggcccac cgggcccgag cttcaggccg tagaacccaa gaaagcgatc
      181 acgggcgctg gccacgatac gccgctgcac acgctgggtc tccgggtcaa gcgacggctc
      241 aagggtgaac ggtctgcgtt cgcctgccat gaggccccgg tcgttgggcg cgaacagcca
      301 ggccgcgtca gcgaagaccg ggtggtcggc cataccccac tcgtgccatt catcaggacg
      361 gtactcggcc agatgccaga acgtccggcc cgcgatctca atcggccgcc acgcgtcggc
      421 gattctgaag gccgtgtcgc gcccgccgag cgggcggcac gcgggctcga gttcagcctc
      481 gcgggcacgg atgctgatgt tgtccagcat gaagccccag cggctgtccg gtcccaccgc
      541 cgccgcgtcg aaacggatcc tcccgcgcac catggggtcg tgtgggaatg cggcccgcca
      601 gcagatgcgg cccgtgtcgc gttcggcaac gatgatcacc gccgtggtgt cctggcagaa
      661 gacggtgacg tggtaccacc ggtcatcctc gggggcgagg cgtgccccag agcgccggca
      721 gtcccaccgc ccgccgtagt ggctccatgc gccaccggag tactcgacgt accaggcggc
      781 gccgtcccac tcctgctccg gcgcactctg cgtcacctgg cacgatacgc agttggcata
      841 gcctcccagg cgctccgccg cgggctgcag gtagtcgaac tcgaccacat ggaacggagg
      901 cacctcggcc agtgaggtga gggtgcatcc accgcctatc ccgagtaggt gcaggacgcc
      961 attctcgacg cgaacgaacg agcccggcgt ctcgctggcg tgcacgtccc acttgctggc
     1021 atcgacaccg gagcccccga agtcgtcctc gaggatggac cgagcgccca atggggcaca
     1081 cagggtgacc agggccacga caaggaaacc agtgagcatg atgttgacca ctgatggtac
     1141 ctccgtccgc tcaggcggtg agaaactgca ctgcaacgcg gtgacgcccg atgggcaccg
     1201 ggaagtaggc ccagttggac gcgcattcgg tcacgcgttt cgggatcaca tccgcggatt
     1261 cgacgaagca acgcgcgccg acggattggc ccagacacac ccggatgacg caccccgatg
     1321 ggtcgggaca gtcgacgaca aactcgagca ggttcccgtc gaagagggcg tcctcgaccg
     1381 tagtcgcgtc gcggttcatc caccactcgt tgagtcgatc gctgcccatg tatgccaccg
     1441 tggccccgag ctcgtcgaca tagcgaagaa gctcatcgat ggcctcgcgg cacccgggag
     1501 aaccggcgat gtagaccggg tggtagaaca ggcacatcgt gagacgaccg gcgagggcca
     1561 gatcgaccgc acgacgtacc gcggcgggat cgcactgctc gtcctggtac ccgacttcgt
     1621 accccgtgat ggcctcctgg acgaagggca ggcgccggtt accgtggcgc cagtcgtccc
     1681 acacgtgccg ggggaaagca gtaccgaaag agaatccggt cacgttggtg gggttcatcc
     1741 cgacgggctc gggaccgaac cacgagttgt cgccggtccc acccgtggcc gcgatccacc
     1801 gggcgggatc ggcccacccg caccatcggc accagtggtt caccgagcac cgcgagcttc
     1861 ggccgaatgc ccgcatgaac tggtctgctt gggcccgcac atcgtgttcg gtgaacccgc
     1921 acggatggct gaacccgtcg acaaagttgt agtgcagcgc gagctcgtgg ccgttggcct
     1981 cgatgtgagc ggcctcctgg gcgctcaccg cgaacccctc accgacgggc atcaggttga
     2041 tgtgataggg caggccccgg ctccgcatga agtccgacgc gggcacctga ttgccggcct
     2101 cgccctcatc gtcgccgccg aagtagaaca cggcgtccgc cacccgccct ccggcgggtg
     2161 ggagggcgtc aaccatgggg attggcctga ggcccaggat gacccggagg agtgattgga
     2221 gcacgtcggt gtacggcacg gccacatcgt tgtcgccgac aatgcgggcg tccatcgctc
     2281 gcaggtagcc atcgccatca tagtcgcggt cgatcggtct tccctggtgg atcgcccaca
     2341 tcgtctgggc gacatcgaag gcaaaatagc aggcgtagcc cgccccgaca cgccggagcg
     2401 taaccccggc gtacggggtc tccacagcgc gcctgccaca ggttggcgca tcgggcgatg
     2461 gctccatcac cgttgcgaga gtctcgacgc cctcatcgtc agcaaccacc agccgcacgg
     2521 gggaaaccac
//
LOCUS       DAOSPL010000635         2527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_41135_length_2527_cov_3.018608, whole genome shotgun sequence.
ACCESSION   DAOSPL010000635 DAOSPL010000000
VERSION     DAOSPL010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41135_length_2527_cov_3.018608"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="PLQ54_11720"
     CDS             complement(<1..1012)
                     /locus_tag="PLQ54_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HOS93974.1"
                     /translation="MRACVLHAIGDLRAGEVSDPVPGPGQVRVRVAACGICGSDLPRV
                     FEKGTYRFPLIPGHEMAGVVEKVGAGVARVAPGDRVAVFPLLPCGWCEPCRLGWLHLC
                     DDYGYLGSRCDGGFAELVLAPEANLVPVPEGVSLEHAALTEPAAVARHALRRGGLAKG
                     ETVWIVGAGTIGLVLAQWAHELGASQVFVSDVQKFKLDLARRWPGVLVCDAREHDAPG
                     WVRDETHGRGADLVVEAVGMAVTAAQAIRGARKRGRVVLMGNPAGEMVLPQNDYALIL
                     RHELDVVGTWNSDRGAGGEGDWAESLRAMCEGALDLDPIVTHRTPLEGVPSLMERMRR
                     GEE"
     gene            complement(981..1952)
                     /locus_tag="PLQ54_11725"
     CDS             complement(981..1952)
                     /locus_tag="PLQ54_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOS93975.1"
                     /translation="MAALTLELGVKTDPIEYRYSFGWLFDLMAQEGVWNVQVGTFFEI
                     YHLPDHWFTSLAEQARRRGLRIRSVFTAHRELGGFYRSDPCWHAVARRNYERLIEVGA
                     LLGAESVGSNPGAVMRDAMDGKGAGIRRYLEHMKELMHFAHDRGVSRLTLEPMSCAAE
                     PPALPGEIRSMCQELVAYHRDHAGSTAGVGLCADVSHGYADEEGAVVHTHLELLEAAL
                     PYTTEVHLKNTDATFGSTFGFTEPERARGIVDVRVVRELLRRRERELPVHQLVGYLEI
                     GGPKTGRDYSDRLLEEQLRGSLRHLRATWSDGEGVRSDESLCSARHW"
     gene            complement(2073..>2527)
                     /locus_tag="PLQ54_11730"
     CDS             complement(2073..>2527)
                     /locus_tag="PLQ54_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93976.1"
                     /translation="PDAVAQRVLAKEPMPNEAGEAEIRERARDILHRYRDDVAADAIG
                     ELQSRIGAGGRGAAGAGPVVEALNRRAAEALVLSRSLAVEGFRCAGCGWLQEHDGSPC
                     PTCGGELRPGLLARLMAQAARGQGASVLFVEDSARMAPFGGVAAFLRF"
BASE COUNT          433 a          946 c          811 g          337 t
ORIGIN      
        1 gctcttcgcc gcggcgcatc cgttccatca gcgatggcac accctccaac ggcgttcggt
       61 gggtcacgat gggatccagg tccagagcac cctcacacat ggcgcggagc gactcggccc
      121 agtccccctc tccccccgca ccccggtccg agttccaggt gcccacgacg tcgagctcgt
      181 gccgaaggat gagtgcgtag tcgttctgcg gcaacaccat ctcaccggct gggttgccca
      241 tgagcaccac acgcccgcgt ttccgggctc cccggatggc ctgggccgcc gtgacggcca
      301 tgccgaccgc ctcgaccacc aggtcggccc cgcggccgtg agtctcatcg cggacccagc
      361 ccggggcatc atgctcgcga gcgtcgcaga cgagaacgcc cggccagcgg cgcgccaggt
      421 cgagcttgaa cttctggacg tcggagacaa agacctggga ggccccaagc tcgtgcgccc
      481 actgggccag cacgagaccg atggtgccgg caccgacgat ccacacggtc tctcccttgg
      541 ccaggccgcc gcgccgcagg gcatggcggg ccacggccgc gggctcggtc agcgcggcgt
      601 gctccaggct caccccttcg ggtacgggca ccaggttggc ctcgggcgcg agcacgagct
      661 cggcgaaacc gccgtcacat cgcgagccca ggtagccgta gtcatcgcac aagtgcagcc
      721 agcccagacg gcacggctcg caccacccac agggcagcag ggggaagacg gccacacggt
      781 cgcccggggc gacacgtgcc acgcccgctc ccaccttttc gaccacgccg gccatctcgt
      841 ggccggggat cagtgggaac cggtaggttc ccttctcgaa gacgcgcggc aggtccgagc
      901 cacagatgcc gcaggccgcc acgcgcaccc gaacctgacc cggccccgga accggatccg
      961 acacctcccc ggcccgcaag tcaccaatgg cgtgcagaac acaagctctc atcgctccgc
     1021 accccctccc catcgctcca ggtcgccctg aggtggcgta gcgaaccgcg gagctgctct
     1081 tcgaggagac gatccgagta gtcgcgcccg gtcttggggc caccgatctc gaggtatccc
     1141 accagctgat ggaccggcag ctcgcgctcc cgccgccgaa gcagctcgcg caccacacgg
     1201 acatcgacga tcccccgcgc ccgctccggc tcggtgaagc caaacgtaga cccaaaggtg
     1261 gcatccgtgt tcttcagatg cacctcagtc gtgtagggca gggccgcttc gagcagctcg
     1321 aggtgcgtat gaaccacggc cccctcctca tcggcgtacc catgggagac atcggcgcac
     1381 aggccgacac cggccgtcga accggcgtgg tcacggtggt aagcgaccag ctcctggcac
     1441 atggagcgga tctccccggg gagcgccggc ggctcggcgg cgcacgacat gggctccaga
     1501 gtgagtcgcg acacgccccg gtcgtgcgcg aagtgcatga gctccttcat gtgctcgagg
     1561 tagcggcgga tccccgcgcc cttgccatcc atggcatccc gcatcaccgc acccgggttc
     1621 gacccgaccg actcggcgcc cagcagggca ccgacctcga tcagccgttc gtagttgcgc
     1681 cgggccacag cgtgccagca cgggtcggac cggtagaagc cacccagttc ccggtgggcc
     1741 gtgaaaacac tgcggatgcg cagaccccgg cgccgggcct gttcggcgag cgaggtgaac
     1801 cagtggtccg ggaggtggta gatctcgaag aacgtgccga cctgaacgtt ccagacaccc
     1861 tcttgggcca tgaggtcgaa cagccatcca aaggaatagc ggtactcgat gggatcggtc
     1921 ttcactccga gttcgagggt cagtgcagcc atggccgggg tgacgcactc catacagccg
     1981 ccgccgtgga acgcaccacg gcggcgatcg gctccatcgc cgtagacatc cgaatgccca
     2041 ggttactggc cgcccggcgc cgcggcaagc cttcagaaac gcaggaaggc ggccacgccg
     2101 ccgaagggcg ccatccgcgc gctgtcctcg acgaacagca ccgaggcgcc ctggccccgt
     2161 gcagcctgtg ccatcaggcg agccaggaga cccgggcgaa gctcgccgcc acaggtcggg
     2221 cagggcgacc cgtcgtgctc ctgaagccac ccacacccgg cgcagcggaa cccctccacg
     2281 gccaagctcc tcgagagcac gagcgcctcg gcggcgcgcc ggttaagtgc ctccacaacg
     2341 ggcccggcgc ccgcggcgcc tcggccgccg gcgccgatac ggctctggag ctcgccgata
     2401 gcatcggcgg ccacgtcatc ccggtagcgg tgaagaatgt cgcgggcacg ctcgcggatc
     2461 tcggcctcgc cagcctcgtt tggcatgggc tccttcgcca gcacgcgctg cgccacggca
     2521 tcaggca
//
LOCUS       DAOSPL010000636         2524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_41188_length_2524_cov_3.293236, whole genome shotgun sequence.
ACCESSION   DAOSPL010000636 DAOSPL010000000
VERSION     DAOSPL010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41188_length_2524_cov_3.293236"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PLQ54_11735"
     CDS             <1..356
                     /locus_tag="PLQ54_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93977.1"
                     /translation="QDIGLALSSWAGYGVAARFRDCVWRDVALDGSDLPLLLLDWDRE
                     VERVVGGAEFSRCVVEDSLDRPALGVHANVAPRTVGDLHGDLTIRNPHGGRIDLGLAT
                     RDVTLRLRRETPRSR"
     gene            971..1174
                     /gene="rpmF"
                     /locus_tag="PLQ54_11740"
     CDS             971..1174
                     /gene="rpmF"
                     /locus_tag="PLQ54_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012938743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOS93978.1"
                     /translation="MICPKNKTSKARTRSRKANWRLVAPNLSSCPSCRALKAPHMACA
                     KCGYYNGRAIIEIKDDTKKKRRR"
     gene            1232..2353
                     /gene="plsX"
                     /locus_tag="PLQ54_11745"
     CDS             1232..2353
                     /gene="plsX"
                     /locus_tag="PLQ54_11745"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOS93979.1"
                     /translation="MRIAVDAMGGDRAPEEAVKGAVNAATRLGVEVSLVGDRSALESC
                     VAGCGSYDSKLISIVDASQVVGMDEPGATAVRRKRDASICVATRLVKDGHADAVVSAG
                     NTGATLSAAMLYLRCIPGVERPGIASVFPTVDGRVLCLDLGANVDCRPRQLVDFAVMG
                     QVYSHRVLGVERPRVGLLNIGEEECKGNEVTKEAYALLQHDPRFEFSGNCEGDELFSG
                     KFDVVVCDGFVGNVVLKTTEGAASLLFESVRREVLSSALSRLAGLLVRPLMRRVVKRL
                     AYEEYGGALLLGVNGVCVIGHGRSSAKAFESALSFAASAVSQDIRGHIERSLAAGGTA
                     WSGAASRDGSDGRSSATADTRPEGRDSRRRPLHSTEGAD"
     gene            2265..>2524
                     /locus_tag="PLQ54_11750"
     CDS             2265..>2524
                     /locus_tag="PLQ54_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93980.1"
                     /translation="MDAAQRLQTPGRRAAIRGVGHYIPQKVLTNADLERMVDTSDEWI
                     TERTGIRERRIAADHETASTMGTEAARQALDAAGVSPDRLDL"
BASE COUNT          400 a          749 c          919 g          456 t
ORIGIN      
        1 gccaggacat agggctggcg ctgtcgtcgt gggcgggcta cggcgtggcc gcgcggttcc
       61 gcgactgcgt gtggcgtgac gtagcactcg acggctccga ccttcccttg ctcctcctgg
      121 actgggaccg tgaggtcgag cgcgtggtgg gtggcgcaga gttcagccgc tgtgtcgtgg
      181 aagacagcct cgaccggccg gcgttgggcg tccacgcgaa cgtggcaccg cgcaccgtgg
      241 gtgacctcca cggtgacctg acgatacgaa acccccacgg tgggcgcatc gatcttggcc
      301 tggcgacccg ggatgtgacg ctgcggttgc gccgcgagac gccgaggtcg cggtagggcg
      361 gcggcacggg gcgggactgg ggcacggcgc cacttccacg cgaacgggcc ctgcatggca
      421 gtgcgcggcg cgcgggagcc gatgacagtc gcgggcggtg cacactgagg ctctgcggcc
      481 aggctcgcag ggggccacac actctacgac actggcccga cttcaattgg tgatatgata
      541 tgtatttgat cttccgggcc aggccgggtg cttatggtga tattttattt atctttttag
      601 cctgggtgtt cataagtttc gccagacggg gggagtggcc gttccttgac ggcggctcgc
      661 cggcgttgcc gccgggcgcg ctcagggcga cgcagtccct cacccgccgc gcggacggcc
      721 gttgccgttg acgttgcacc cgcaggcttg caggcttgag ccgccttgac agtggtcgag
      781 gccaagggta cagtggtgtg tctcatcgct cggcagcgtg ccgccgaagg ctgcctcgcc
      841 tcaggcccca ctggccaacc gcgcccgcga gtcatccggt gcgtgtgttt tcatgtgtgg
      901 cgagtcggct gccgggtttt tccgtcgctg gtgaagtgtc tgccgtgatg gatgggggag
      961 gaaagctggc gtgatctgcc cgaagaacaa gacatcgaag gcccggacgc ggtcgcggaa
     1021 ggccaactgg cgcctggtgg cgcccaatct ctcgagctgt ccgagctgcc gagccctcaa
     1081 ggctccgcac atggcatgcg cgaagtgcgg ttactacaac ggccgggcta tcatcgagat
     1141 caaggacgat acgaagaaga agcgtcgccg ttagccccgg ttggggcctc gcccgccacg
     1201 ccgcgtgtgc gggccaacag gcaggggctc tatgcgcata gctgtagacg caatgggcgg
     1261 tgatcgggcg ccggaggaag cggtcaaggg ggcagtcaac gccgcgactc gcctcggcgt
     1321 tgaggtaagc ctcgtcggtg atcgctcggc gttggagagt tgtgtggccg gttgcggctc
     1381 gtacgactcg aagctgatat cgattgtcga tgcgagccag gtcgtcggga tggacgagcc
     1441 cggtgccacc gcggtccggc gcaaacggga cgcatcgatc tgtgtggcga cgcgactcgt
     1501 gaaggatggc catgccgacg cggtggtctc ggcgggtaac acgggggcca cgctgtcggc
     1561 tgccatgctt tacctccgct gcattccggg tgttgaacgc ccaggcatcg cctcggtctt
     1621 tcccacggtg gatggacggg ttttgtgtct cgacctgggc gccaatgtgg actgccggcc
     1681 ccgccagctg gtggatttcg cggtaatggg gcaggtgtac tcgcatcgcg tgctgggagt
     1741 cgagcggcca cgcgtcggac tgctgaacat cggcgaggag gagtgtaagg gcaacgaggt
     1801 cacgaaggag gcctatgccc tgctccagca cgatccccgt ttcgagttct cgggcaactg
     1861 cgagggcgat gagctgttct cgggcaagtt cgatgtggta gtctgcgacg gatttgtggg
     1921 caatgtggta ctcaagacga ctgagggagc cgccagtctc ttgttcgagt cggtgcggcg
     1981 tgaggtgctg tcatcggccc tttcccgcct cgcggggctc cttgtgcgcc cactgatgcg
     2041 acgcgtcgtc aagcggctgg cctatgagga gtacggcggg gcgttgctgc tcggcgtgaa
     2101 cggtgtgtgc gtgatcggcc atggaagatc gagcgcaaag gcgttcgaga gcgccctgtc
     2161 attcgccgcg agtgcggtga gccaggacat ccgagggcac atcgaacgct ctctggccgc
     2221 gggcggaacc gcatggtccg gcgcggcctc acgggacgga tctgatggac gcagctcagc
     2281 gactgcagac acccggccgg agggccgcga ttcgcggcgt cggccactac attccacaga
     2341 aggtgctgac taacgccgat ctcgaacgga tggtcgacac ctccgacgag tggatcacgg
     2401 aacgcaccgg gatcagggaa cgccgaatcg cggcggatca cgagacggcg tcgacgatgg
     2461 gcaccgaggc ggcccggcag gccctggatg ctgccggagt gtcgcccgac cgactcgacc
     2521 tcat
//
LOCUS       DAOSPL010000637         2524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_41191_length_2524_cov_3.154719, whole genome shotgun sequence.
ACCESSION   DAOSPL010000637 DAOSPL010000000
VERSION     DAOSPL010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41191_length_2524_cov_3.154719"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PLQ54_11755"
     CDS             complement(<1..798)
                     /locus_tag="PLQ54_11755"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS93981.1"
                     /translation="MAGIGVGVIGVGVFGENHCRTYADDPGAELLAICDANAARLNEI
                     GDRFGVASRYTDYRELLARDDIQAVSIATPDFLHCEFAVAAAKAGKHILLEKPMATTE
                     AECATIRQAVEQTGVKLFVDFHNRFNPPMVVVKDALQAGELGALQMMYVRLSDAIWVP
                     TGMLSWGAKSTVAWFLACHCVDLIQWLSGSKVVRVYSISRSRVLKARGLDTPDFFQTT
                     LELSNGEVAVVENCWILADSMPSVAHFLLEAVEKGHMEVNALQNTLIR"
     gene            complement(876..>2524)
                     /locus_tag="PLQ54_11760"
     CDS             complement(876..>2524)
                     /locus_tag="PLQ54_11760"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOS93982.1"
                     /translation="GARLCLACAEARQWAVAETHRLMREFKLDYLKHDISPIVTRCNK
                     TGHAHAHTTDVSYWATLGYYRVMESLLASFPSLLLENCSGGGHIKDFGAVRRSHYVVT
                     TDTLSNLPDRQSIYDSTFALPPLVLQAYTYNNAYPVEGDDPGSFLWRSAMMGAWQIDP
                     TDATRWDAEQIACAGRSAAIYKQWIRPLLADAKVHHILPRPDGVHWDGLFEFSASRRK
                     GTLFVFRPDSPEDRQTVRLRGLDPYGSYWIWCEDGSISAPGQHTGRELMDEGLVLSLP
                     ARYSSDLVYVQDAAAGPAPDLSPPGAFRILPTKTDSSAFSASAKLRWTASPNARSYRL
                     RVARNPELATPMVDTIRILTETTLEDLPAGQPLWWCVSAIGWGGRRDGQIVRLDVPPV
                     TPLPGVAFLSDMEWAKATAGAGNPVRRDRNYYDRPLAIGGRKYPKGLWTHAFDDATPA
                     DVVIDIGGKGYGAFVADVGLDDRSGGGSVQFAVWVDDKVAAESPNLTPRQMHRIVVPV
                     SGAHTVTLRVLNGGDGYACDHAVWGLARFIAEGAADPLGE"
BASE COUNT          397 a          833 c          860 g          434 t
ORIGIN      
        1 acggatcagt gtgttctgga gcgcattgac ttccatgtgt cccttctcca cggcttcgag
       61 gaggaaatgc gccacactgg gcatggagtc ggccaggatc cagcagttct cgaccacagc
      121 cacctcgccg ttcgagagtt ccagggtggt ctggaagaag tcgggcgtgt ccagtccgcg
      181 cgccttgagt acgcggctcc ggctgatcga gtagacgcgg acgaccttgg agccagagag
      241 ccactggatc aggtcgacac agtggcaggc gaggaaccat gccaccgtcg acttggcgcc
      301 ccagctcagc atgcccgtcg gcacccagat ggcatccgag agtcgcacgt acatcatctg
      361 cagcgcgccc agttccccgg cctgcagtgc gtccttcacc acaaccatgg gcgggttgaa
      421 gcgattgtgg aagtccacga acagcttcac gcccgtctgc tcaacagctt ggcggatggt
      481 ggcgcactcg gcctcggtgg tcgccatggg cttctcgagc aggatgtgct taccggcttt
      541 ggccgcggca acggcgaact cacagtggag gaagtccggc gtggcgatgg agacggcctg
      601 gatgtcatcg cgcgccagca gttcccggta gtctgtgtag cggctggcca cgccgaaccg
      661 gtcgccgatc tcgttcagcc gcgccgcgtt ggcgtcgcag atggccagaa gctccgcgcc
      721 cgggtcatcc gcatacgtgc ggcagtggtt ctcgccaaag acgccgactc cgatgacacc
      781 cactccgata cccgccatgg tacgcgcctc ccgttgtcgt cagtccgcgc gagggtgttc
      841 cgcggcggcg gtgggaaacc tcgtggcagc ccgggtcact cgcccaatgg gtccgcggcg
      901 ccctccgcga tgaaccgcgc cagtccccag actgcgtggt cgcacgcgta cccgtccccg
      961 ccgttgagca cccgtagcgt gacggtatgg gcaccgctga cgggcaccac gatccggtgc
     1021 atctggcgag gcgtcaggtt gggtgactcg gcggcgacct tgtcatcgac ccacaccgcg
     1081 aactgcacgc tgcccccgcc actccggtca tcgagcccga cgtcggcgac gaaggcgccg
     1141 tatcctttgc ctccgatgtc gatgactacg tcggccggtg tggcgtcgtc gaaggcgtgg
     1201 gtccacagcc ctttggggta cttgcggccg ccgatggcca gcggccggtc gtagtagttt
     1261 cggtcgcgcc ggacagggtt tccggcgcct gccgtggcct tcgcccattc catgtcagag
     1321 aggaaggcca cgccgggtag aggcgtcact ggcggcacat ccagtcgcac aatctggccg
     1381 tccctccggc ccccccagcc gatcgcggac acgcaccacc acagcggctg ccccgcgggc
     1441 aggtcctcca gcgtcgtctc ggtgaggatc cgtatggtgt cgaccatggg cgtggccagc
     1501 tccgggttcc gcgcgactcg caaccggtag ctgcgcgcgt ttgggcttgc cgtccagcgg
     1561 agcttcgccg atgccgagaa cgcgctggag tcggtcttcg tgggcaggat gcggaacgcg
     1621 ccgggaggcg acagatccgg cgccgggccg gcggcggcgt cctgcacgta gaccaggtcg
     1681 ctggagtacc gggccggcag gctgagaacg agcccctcgt ccatgagctc gcggccggtg
     1741 tgctggcccg gtgccgagat cgacccgtcc tcgcaccaga tccagtagga gccgtatggg
     1801 tcgaggcccc gcaggcgcac ggtctgccgg tcctctggcg agtcggggcg gaagacgaac
     1861 agcgtgcctt tgcgccggga cgcgctgaac tcgaaaagcc cgtcccagtg gaccccatca
     1921 ggacgcggca ggatgtggtg caccttcgcg tcagcgagta gggggcgtat ccactgcttg
     1981 tagatcgcgg ccgatcggcc ggcacatgcg atctgctcgg catcccaccg tgtcgcgtcg
     2041 gtgggatcga tctgccacgc gcccatcatg gcgctgcgcc acaggaatga ccccgggtca
     2101 tcgccctcga ccgggtacgc gttgttgtag gtgtaggcct gaagcaccag gggcggcagc
     2161 gcgaaggtcg agtcgtagat gctctgccgg tcaggcaggt tcgagagggt gtccgtggtc
     2221 accacgtagt ggctgcgccg cacggcgccg aagtccttga tgtgccctcc gccggagcag
     2281 ttctcgagca ggagactcgg gaagctggcc agcagtgatt ccatcacccg atagtagccc
     2341 agcgtcgccc agtagctgac atcggtggtg tgggcatgcg cgtgccctgt cttgttgcag
     2401 cgggtgacga tggggctgat gtcatgcttc aggtagtcga gtttgaactc gcgcatgagg
     2461 cgatgggtct ccgccaccgc ccactggcgg gcctcggcgc atgcgaggca cagtcgagcc
     2521 cccc
//
LOCUS       DAOSPL010000638         2524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_41196_length_2524_cov_2.493722, whole genome shotgun sequence.
ACCESSION   DAOSPL010000638 DAOSPL010000000
VERSION     DAOSPL010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41196_length_2524_cov_2.493722"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..1132
                     /locus_tag="PLQ54_11765"
     CDS             161..1132
                     /locus_tag="PLQ54_11765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS93983.1"
                     /translation="MAARGGIRATRRPAHAVDHPRNWNTAELLCRCLETVLASPPGQE
                     AEVVVVDNASIDDSVARVRERFPQVRVIQNPANVGFARANNQGAHECSGDVLLLLNTD
                     TEVREGALEALTDFITSHDDVGIVGPRLVSPDGSVQSSCRTEPGLLNALLEALALTRL
                     APRSRLFGRPEMTWFDHHSALDVDYVSGACLAIRRELWNQLGGFDEGFFFYGEDADLC
                     RRARQAGFRVAYFPEAEVLHLGGASAARVGPRKAIEGYRAALRFARKHHGRAYAVAME
                     AVIALGTALRLVASLPRRGEAARAQRQAYWAVLRWLFRPGARPGAPW"
     gene            1251..2030
                     /locus_tag="PLQ54_11770"
     CDS             1251..2030
                     /locus_tag="PLQ54_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HOS93984.1"
                     /translation="MAAFDIRSVTKWRFVPSGDGGTRRVLALSGVSLEVPERSIMAVV
                     GPSGSGKSTLLRLLNRLEEPDEGTIGFAGVPLRELDVLELRRRVGLVQQQPAMFAGSV
                     ADNVAYGPALRGEWSATDLDRVGALLDTVGLPRDFAPREAQQLSGGERQRVAIARALA
                     NDPEALLLDEPTSALDPGAARHVLEAIRRVSEVAGVTAVVVSHTLAHARAVAQWAAIL
                     VGGQLVEHGPADQSLAAPQSPQARAFIEQAAEAGAEAEHDG"
     gene            2023..>2524
                     /locus_tag="PLQ54_11775"
     CDS             2023..>2524
                     /locus_tag="PLQ54_11775"
                     /inference="COORDINATES: protein motif:HMM:NF015605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS93985.1"
                     /translation="MGDVGYQSIGYPNLALAIGFVVLAVVLARVERLGLEKALVIGSI
                     RACVQLMAMGFVLVLVFEMDRWYHVVPVVILMVLVGARSAYQRVERKPPGLQAVMTGS
                     IAVAVLAVMGPVVTVVLRLEQWYQPQYLIPISGMIIGNATTGCAVAADRLLAELTLRR
                     DEVEAML"
BASE COUNT          334 a          848 c          926 g          416 t
ORIGIN      
        1 actgctctac cacatgctga tcgccacggt ttccgtgatc gtgctgatcg ccttcacgtg
       61 catctacctg aacgacaagg ccaagggcgg gtacaaggcc gagaagcttg gtgcggagta
      121 gggtgaattg gggggccgct gatgcggccc ccgatgcctg gtggccgcca ggggtggtat
      181 acgcgcaacc cgacgtccgg cccacgctgt cgatcatcct cgtaactgga acacggcgga
      241 gctgctgtgc cgctgcctgg agaccgtgct tgcctcgccc ccggggcagg aggctgaggt
      301 cgtcgttgtc gacaatgcct ccatcgatga cagcgtggcc cgtgttcgcg agcggttccc
      361 gcaagtccgg gtcatccaga acccggccaa tgtcggtttc gcgcgcgcca acaaccaggg
      421 cgcccacgaa tgctcgggcg acgttctgct tctgctgaac accgacaccg aggtgcgcga
      481 gggcgcgctg gaggccctga cggacttcat aaccagccac gatgatgtgg gcatcgtcgg
      541 cccccgcctc gtctctccgg acggcagcgt gcaatcgtcc tgccgcaccg agcccggtct
      601 gctcaacgcg ctcctcgagg ctctggcgct gaccagactg gccccgcggt cgcggctgtt
      661 cgggcggcca gagatgacgt ggttcgacca tcattcggcg ctcgatgtcg attacgtcag
      721 cggggcgtgc ctggccatcc gccgtgagct gtggaaccag ctcggcgggt tcgacgaggg
      781 gttcttcttc tacggggaag acgccgatct atgccggcgc gcccgccagg ccggcttccg
      841 cgtggcctac ttccccgagg ccgaagtgct gcacctgggt ggcgccagcg ccgcccgagt
      901 tgggccccgg aaggccatcg agggctaccg cgccgcgttg cggtttgcgc ggaagcacca
      961 cggccgggcg tacgcagtgg cgatggaggc cgtgatcgcg ctgggcaccg cgctacggct
     1021 ggtcgcgtcg ctccctcgcc gcggcgaggc ggcccgcgct cagcgccagg cctactgggc
     1081 ggtgctgcga tggctgtttc ggcccggagc tcgccccgga gcgccctggt agcgttcgcc
     1141 tcggcgggtg ttcggcgggg ccgagcggag gggcctgcgg agcgggaagg gcgccatgcg
     1201 gcccgccggc acgggcaggc tagcggcggc acaccgtgga gggacggccc atggctgcct
     1261 tcgatatccg gtcggtgacc aagtggcgct tcgttccgtc gggcgacggc ggcacgcggc
     1321 gcgtgctcgc tctgtcgggc gtcagcctgg aggtgcccga acggtccatc atggccgtgg
     1381 tgggcccatc aggctcgggc aagtcgaccc tcctgcgcct gctcaaccgg ctcgaggagc
     1441 cggacgaggg caccatcggc ttcgccggag tcccacttcg cgagctcgac gtgctcgagc
     1501 tccggcggcg cgtcggcctc gtgcagcagc agcctgccat gttcgccggc agcgtggccg
     1561 acaacgtggc ctacgggccg gccctgcgtg gcgagtggtc ggcgacggac ctggatcggg
     1621 tcggcgcatt gctcgacacg gtcggcttgc cccgcgactt cgcgccgcgc gaggcgcagc
     1681 agctctccgg cggcgagcgg caacgggtgg ccatcgcgcg ggcgctcgcc aacgacccgg
     1741 aggcgctgct gctcgatgag cccacctcgg ccctggatcc cggggcggcg cggcacgtgc
     1801 tcgaggccat ccggcgcgtg agcgaggtgg ccggcgtgac cgcggtcgtc gtgagccaca
     1861 cactcgccca cgcacgggcc gtggctcagt gggctgcgat tctggtcggc ggccaactgg
     1921 tcgagcacgg cccggcggat cagtctcttg ccgcgccgca gtcgccacag gcccgcgcgt
     1981 tcatcgagca ggcggcggag gcgggagcgg aggccgagca cgatgggtga cgtgggctac
     2041 cagtccatcg ggtatccgaa cctggctctg gccattgggt tcgtcgtgct ggccgtcgtt
     2101 ctggcgcgcg tggagcggct ggggctcgag aaggccctcg tcatcggctc gatccgagcg
     2161 tgcgtgcagc tcatggccat ggggttcgtc ctcgtcctcg tgttcgagat ggaccgctgg
     2221 taccacgtcg taccggtcgt gatcctgatg gtgctggtcg gagcccggtc ggcctaccag
     2281 cgcgtcgagc gtaagccacc gggactccag gcggtcatga cgggctccat tgccgtggcg
     2341 gtcctggccg tcatgggccc ggtcgtgacc gtggtcctgc gcctggagca gtggtaccag
     2401 ccgcagtacc tgatcccgat ctcgggcatg atcatcggca acgcgacgac agggtgcgcc
     2461 gtggcggcgg atcgccttct ggcggagctc accctgcggc gagatgaggt cgaggccatg
     2521 ctgt
//
LOCUS       DAOSPL010000639         2523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_41213_length_2523_cov_3.329417, whole genome shotgun sequence.
ACCESSION   DAOSPL010000639 DAOSPL010000000
VERSION     DAOSPL010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41213_length_2523_cov_3.329417"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLQ54_11780"
     CDS             complement(<1..346)
                     /locus_tag="PLQ54_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93986.1"
                     /translation="MGPALALLILPIAAAPAKGPIAMTGALAAMPDGYHLQRFDDCGV
                     TGRQPHTIAEGVHEYSPGAVNADQRSRTVAWGWREVRAAYTDLDPALDYVLAVTYCNE
                     PYNHRVQSLWAGE"
     gene            575..1654
                     /locus_tag="PLQ54_11785"
     CDS             575..1654
                     /locus_tag="PLQ54_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS93987.1"
                     /translation="MTGRERLSAIMHRQKADRLAWTILVDDITLSVLPERVGVRTGFE
                     FARYLGCDVFQLEGWGTPHGFRSPVLEWGSGIEERRFRRDGNDVTELRSSDGVLSAEW
                     RGGHPVKHAVGSLEELGLYTAMWETARFVPADDSEAFSAVDAAVGDAGIITRFWGPST
                     VPRLLEYDMGMASFYYLLHDHPAEMERLFRVIHEREVVAFQELAAGPCDVITLVENTS
                     SAYISPDTYRRYNAPHVRDFVDVVHAAGKVALIHMCGHVMRLLPTIRTTKLDGIHALT
                     PPPTGDTPWEMALDIVGEDLVVIGVLDPSIFVMGPVEGIGPALDQLYTRRIRRSNSVL
                     CLAADGIPVPIERFEAVAQWMEANG"
     gene            1714..>2523
                     /locus_tag="PLQ54_11790"
     CDS             1714..>2523
                     /locus_tag="PLQ54_11790"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93988.1"
                     /translation="MRLLVVVLLGMVAGGLCPALASSAAESWPRSAVRPRVDLDGAWD
                     FRMDPGAVGERDGWFRRGATFPDRIQVPGSWEAQGFGDPTDKVYHNYVGKAWYRRHVA
                     IPREFAGKRAILHVGAVHRSAKVWVNGHHVGGHIGYVTPFEIDVTDQVRPGRTALLII
                     QVDSAQDWEIDTLTGCVDLIDYMFVNWGGIWHHVWLEARDTTCIDNSFVKPDVARSVA
                     SVEVELTNQGSRASRTLVVEALHPDGDRAGRTETIVTLEPGTTSASVDVPVS"
BASE COUNT          374 a          843 c          872 g          434 t
ORIGIN      
        1 gctcacccgc ccacaggctc tgcactcggt ggttgtaagg ctcgttgcag taggtcaccg
       61 cgagcacgta gtcgagggcc gggtccaggt ccgtgtatgc cgcccgcacc tcgcgccagc
      121 cccaggccac cgtgcggctc cgctggtcgg cgttcacggc gccggggctg tactcgtgta
      181 ccccctcggc gatggtgtgc ggctgccggc ccgtcacccc gcagtcgtcg aaccgctgga
      241 ggtgatagcc gtcaggcatg gcggccagag cgcccgtcat cgcgatcggt cctttcgccg
      301 gggcggcagc gatggggaga atgaggagag ccagagccgg tcccatggcg gcctccgacc
      361 ttcgggggat gagcagtggg tgctcgcccc ctgttccgcc cagaagcggc ggacacctcg
      421 cccgccaccg gcatgagccg ccggagagct gagctgccgc caacgaaggg acgggtctcc
      481 cggcggcgtc cggggccgag cccgagggcc atcgggcctc cacgaaggcc gcgccgtctg
      541 catggcgaac tccgcctccc acggagggat gcccatgacc gggcgcgagc ggctgtctgc
      601 catcatgcac cgccagaagg ccgaccgtct ggcgtggacc attctggtcg acgacatcac
      661 cctctcggtg ctgcccgagc gtgtgggcgt ccggaccggt ttcgagttcg ctcggtatct
      721 gggctgcgat gtcttccagc tcgagggttg gggcacgccg catggtttcc gctcgcccgt
      781 cctcgagtgg gggtcgggta tcgaggagag acgcttccgg cgcgacggga acgatgtgac
      841 tgagctgcgc tcgtccgatg gcgtgctctc ggccgagtgg cggggcggcc atccggtgaa
      901 gcacgccgtc ggcagtctgg aagagctggg cctctacacg gccatgtggg agaccgcccg
      961 cttcgtcccg gccgacgact cggaggcatt ctcggcggtc gatgccgccg tgggcgacgc
     1021 cggcatcatc acgcgcttct gggggccgtc cacggtcccg cggttgctcg agtacgacat
     1081 gggcatggcc agcttctact acctgctgca cgaccacccg gccgagatgg agcgactctt
     1141 ccgggtcatc cacgagcgcg aggtggtggc cttccaggag ctggctgccg ggccctgcga
     1201 tgtgatcacg ctggtcgaaa acaccagctc ggcctacatc agtccggaca cctaccgccg
     1261 gtacaacgcg ccccacgttc gcgacttcgt cgatgtggtt catgcggccg gcaaggtcgc
     1321 cctgatccac atgtgcggcc atgtcatgcg gctgctgccc accatccgca cgacgaagct
     1381 cgatggcatc cacgctctga cgccaccgcc cacgggcgac accccttggg aaatggccct
     1441 ggacattgtg ggagaggatc tcgtcgtcat cggcgtcctg gatccctcga tcttcgtgat
     1501 gggtccggtc gagggcatcg gcccggcgct ggaccagctc tacacgcgcc ggatccgccg
     1561 gtcgaactcg gtgctgtgcc tggcggccga tggcattccc gtacccatcg aacgtttcga
     1621 ggccgtagcc caatggatgg aggcaaacgg gtgagagctg gtcacgttgg ccggccgttc
     1681 gaccgggccg aatgccgaga taggtggtgc gcgatgcggc ttctcgtggt agtgcttctc
     1741 ggcatggtgg caggtgggct ctgtcctgcc ctggcctcgt cggccgcgga gtcgtggccc
     1801 cggtcagctg tgcggccgag ggtggacctc gatggcgcgt gggacttccg catggaccct
     1861 ggggcggtgg gggagcgcga cggctggttc cgccgtggcg ccaccttccc cgaccgtatc
     1921 caggtccccg gctcatggga ggcccagggc ttcggcgacc cgacggacaa ggtctatcac
     1981 aactacgtcg gcaaggcctg gtaccggcgc catgtggcca tccctcgcga gttcgccggc
     2041 aagcgggcca tcctgcatgt tggcgccgtg caccgctccg ccaaggtctg ggtcaacggc
     2101 catcacgtgg gggggcacat cgggtatgtg acgcccttcg agatcgatgt gaccgaccag
     2161 gtccgccccg gccgaaccgc tctgctcatc atacaagtcg atagcgctca ggattgggag
     2221 atcgataccc tcacgggctg tgtggacctg atcgactaca tgttcgtgaa ctggggcggc
     2281 atttggcacc acgtgtggct cgaagcgcgc gacacaactt gtatagacaa ctccttcgtg
     2341 aagcccgatg tagcccgctc ggtcgctagc gtcgaggtgg agctgacgaa ccagggctcc
     2401 agggcgtcgc ggaccctcgt cgtcgaggcg ctccatcccg acggtgatcg tgcggggcgc
     2461 accgagacca tcgtgaccct cgagccgggc acgacgagcg cctcagtcga cgtgccggtg
     2521 tca
//
LOCUS       DAOSPL010000640         2522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_41231_length_2522_cov_3.138630, whole genome shotgun sequence.
ACCESSION   DAOSPL010000640 DAOSPL010000000
VERSION     DAOSPL010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41231_length_2522_cov_3.138630"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLQ54_11795"
     CDS             <1..321
                     /locus_tag="PLQ54_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93989.1"
                     /translation="LPNLAAAANDMGEWKSVFIGGLGLTDEFVHAMARWAGCWCTAEP
                     GDAVYANESFITIHALYPGHKVLTLARPSRVVDLTSGETLGEGLSIIELDMERAETRW
                     FALE"
     gene            complement(384..545)
                     /locus_tag="PLQ54_11800"
     CDS             complement(384..545)
                     /locus_tag="PLQ54_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93990.1"
                     /translation="MGRGSLRPYLFVFTAQFRMLLQCRAIAVASLGFLLASLWTWQFG
                     VRHYRSTGS"
     gene            842..2374
                     /locus_tag="PLQ54_11805"
     CDS             842..2374
                     /locus_tag="PLQ54_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93991.1"
                     /translation="MTVTRHWAALLAGWCIPLVLAAAPDPAAAVNVALITERAGANSV
                     EVATEAGLGRNIVPACKMKGFQWSWDGTRLAYVTPKDDSLELHAVDIPAAIDTVVAAS
                     KDLVSMRWAPDCKHLAYATYDAAAKVGHIRVTDVTAPAGTAPLDASGTLNPGISGDYA
                     FSHDSRFLVFGVPKATDITVGEHKIPQRTCAIADLARGTVLELPRLNALYWAWSPTAP
                     EFGLLATVGEDLTRQLMIVDVTDGQVKPPVRVSKLGDQLLADVTAFDWSPVGGHLAFR
                     TALRTSREDERATEGRVFLVNTRTKVSTMVQGTKNAINFAWAPNGQAFLLEDADPESG
                     AGKERLRIGQLSDPLTGDSWLVPEAVGIATTKLAWSPDSSALGYIRQGAIYVVDLATR
                     KTRLWCDSVNVEAISWVPDGKSVVAIMRSPKGDSLSISQVVPREAEGSATVRRVSGEF
                     RCSVSMDWAPDGVHLLALAHLNADTAKGLLFSLRREGERPLEVSLSNAVVSASWFPKV
                     KP"
BASE COUNT          417 a          771 c          899 g          435 t
ORIGIN      
        1 cttccgaacc tggccgcggc cgcgaatgac atgggcgagt ggaagtcggt ctttatcggc
       61 gggctcgggc tcacggatga gttcgtgcac gccatggcgc gatgggccgg gtgctggtgc
      121 acggcggagc cgggcgatgc ggtatacgcc aacgagagct tcatcaccat ccacgcgctg
      181 tatcccggcc acaaggtgct gacgctggct cggccgtcgc gggtcgtgga cctgacctcg
      241 ggcgagacgc ttggcgaggg gctatcgatc atcgagctgg acatggagcg ggccgagaca
      301 cggtggttcg cgctcgagtg atcgaccggg ggcgtgccgg ggctccactg ccttctgggc
      361 agtcatgcac gcccgtcttg gtcctagctt cccgtcgagc ggtagtgacg gacgccgaac
      421 tgccaggtcc agagggatgc gaggaggaag ccgaggctgg ccacggcaat ggcgcggcac
      481 tgcagcagca tgcggaactg ggcggtgaag acgaagaggt agggtctcag agagccgcgc
      541 cccatccggt gctcgcctcg ccctcgtaga accggccgat gatcccctcg atgggcgggc
      601 tctcgacgag gatttgcggc atgggtgtca aggtcgagct gccgagcgag gcgcgggcgc
      661 gattctcggt tcggggccct tctgctatac tcccccgggg cccgcgcggc cctttcccag
      721 tgttctgtgg agcctgatcg ggctgtctcg cgtctaacca atggcgtcgg ccctggacga
      781 cccggccgct tcgccatctc acccatatca tgctgccgcg cggcagccag gaagagggac
      841 gatgaccgtg acgagacact gggcggcgct gctggctggg tggtgcattc cgctggtgct
      901 ggccgcggcg ccggatccag cggccgccgt caacgtggca ctgatcacgg agagagcggg
      961 ggcgaactcc gtcgaggtgg ccactgaggc cggactgggc cggaacatcg ttccggcctg
     1021 caagatgaag ggcttccagt ggagctggga tggtacccgt ctggcctacg tgacgcccaa
     1081 ggacgattcg ctggagctcc atgcggtgga tatccctgcc gccatcgaca cagttgtcgc
     1141 ggcaagcaaa gacctggtgt cgatgcgctg ggcacccgac tgcaagcatc tggcctacgc
     1201 gacctatgat gccgcggcga aagtggggca tattcgggtg accgatgtca ccgcgcccgc
     1261 gggcacggca ccgttggatg cgagtggtac actgaacccg gggatctctg gtgactacgc
     1321 tttctcccac gattcacgct tcctcgtgtt cggcgtgccc aaggcgaccg atatcacagt
     1381 cggcgagcac aagataccgc agcgcacgtg cgccatcgcc gacctggcgc ggggaacggt
     1441 gttggagctg cccaggctga acgccctcta ctgggcctgg tcgccaacgg cccccgagtt
     1501 tgggcttctg gcgaccgtgg gcgaggacct gacgcgccaa ctgatgatcg tcgacgtcac
     1561 cgatggccag gtgaagcccc ccgtgcgagt cagcaagctc ggagatcagc ttctggcgga
     1621 tgtcacggcc tttgattggt cgccggtggg agggcacctt gctttccgga cggccctgcg
     1681 gacgtcgagg gaagacgaga gggccaccga gggacgcgtg ttcctcgtga acacgcgaac
     1741 caaggtatcg accatggtgc aaggcaccaa gaacgccatc aacttcgcct gggcgcccaa
     1801 cggtcaggca ttcctgcttg aggacgcgga ccccgagagt ggagcgggca aggagcggct
     1861 tcgcatcggc cagctcagcg atccactgac cggcgactcg tggctggtac cggaggcagt
     1921 ggggatcgcc accaccaagc tggcgtggtc gcccgacagt tccgcactgg ggtacattcg
     1981 gcaaggggcc atctacgtgg tcgatctggc gacgcggaag acccgcctgt ggtgtgacag
     2041 cgtcaacgtc gaagcgattt catgggtgcc ggacggaaag tctgtggtgg cgatcatgcg
     2101 cagtccgaag ggggactcgc tcagcatatc gcaggtggtc cccagggagg ccgaaggctc
     2161 ggccacagtg cgccgcgtca gcggcgagtt ccgatgctcg gtgagcatgg actgggcccc
     2221 cgacggcgtt cacctgttgg cgctggcgca tctgaacgcg gatacggcca aggggctgct
     2281 gttctcgttg cgtcgcgagg gggagcggcc gctcgaggtg tcgctgtcca acgcggtggt
     2341 ctccgcctcg tggttcccga aggttaagcc atagcctgat gtcattctgc ggctcaccga
     2401 ctcgtgggga ttcgttcatg actagacaag gtacgcgctg gcgcaaggtg cgctggctcg
     2461 cggtcgcggc catggcctgg tgcggcgtcg ggttgccagc cctgcatggg caccacgcgg
     2521 ta
//
LOCUS       DAOSPL010000641         2521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_41253_length_2521_cov_2.805353, whole genome shotgun sequence.
ACCESSION   DAOSPL010000641 DAOSPL010000000
VERSION     DAOSPL010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41253_length_2521_cov_2.805353"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLQ54_11810"
     CDS             <1..194
                     /locus_tag="PLQ54_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93992.1"
                     /translation="PDGQPAVLIVNKDLHRSTAFGLEFKQPGRVMLVSPYNGSKQPFG
                     GEHCWLAAGQGALLLLEKP"
     gene            191..>2521
                     /locus_tag="PLQ54_11815"
     CDS             191..>2521
                     /locus_tag="PLQ54_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93993.1"
                     /translation="MRTARTAVGVALLGLLVLPLRADEPLEAARSLFLNLASRLFVDE
                     LDVAGPPAGVEAPWAAKIAWRQAGGRPEWGNVTLGFRADAATGYSFDLLVESATPGSG
                     LNVYLTEADADRWVEAFPLADRVGQGWAHIDLQGDALRLWQFGNGVREPATVGGLTLE
                     PYGKDGALVVWVANLAIHTPAGLRDALSLMADSDCPPPTAVGPVTRLAEVPRCYMGMS
                     LSFLAKPEGQELVAEVRKLVPSLGVAAAGAQWRPEWAEAIARVRADGVDVRVQNGGIA
                     GMAGLITRARAWGVDPQGRSRNTTPGYGDGMHSVSYCHPAAREAVRRMMAAMRGMGIL
                     DYEQIDYVWPWQGGPWGYGEWDREAFRTDLLGQDEGLRLASGETVHFSDYLAAYGVDR
                     IGPGDLGIATWGLFEPKAAEGLPEDARRRHSFVFTALTHYEWLKFAQAMGAEARSWGG
                     EFLATLNPEDVSNGGDYVFWSRLRDTGAEYLERFGNPGETEFWRHYLPYLRQEATRSG
                     KRLGVIYEVGVGGHGQPYHDPEVAYCLSYDCTASGGFDDMQNEWMDEAGWAASQEGRH
                     HDRLQQWLATAHAFNQAKADGAALPARRALAIGQRCVVHAIGFSPSYLLTRGALNDLG
                     IDCDYSDLTMDPADWEAYDVLVYTPWESSHRHLEALPRWLDAKPGRMVITHGAVPTRI
                     ASGLGYAPSAAVGDPGLGGLLGLGEITVGGAAEGVVSQVSPGLAAALAPGQAPSLPGG
                     LYQTAQGEAVARIGDAALVTRAARPNGSVVYYLNYAA"
BASE COUNT          360 a          851 c          923 g          387 t
ORIGIN      
        1 atcccgatgg gcaaccggcg gtgctgatcg tgaacaagga cctgcaccgg tcgacggctt
       61 tcggcctcga gttcaagcag ccgggcaggg tcatgctggt gagcccttac aacgggagca
      121 aacagccctt cggtggggag cactgctggc tggcggccgg ccagggggcg ctgctgctgc
      181 tggagaagcc gtgaggacgg cccgtaccgc tgttggtgtg gcccttctgg ggctgctggt
      241 gctgccgctg cgggccgacg agcccctcga ggcggcgcgc agcctgttcc tcaatctggc
      301 gagccggctg ttcgtcgatg agctcgacgt cgccggcccg ccagccgggg tcgaggcgcc
      361 gtgggcggcc aagatcgctt ggcgtcaggc tggggggcgg cccgagtggg ggaacgtaac
      421 cctcggcttc cgcgccgacg ctgccaccgg gtattcgttc gacctgctcg tcgagagcgc
      481 cacgcccggc agcggcctga acgtctacct gaccgaggcc gatgccgatc gctgggtcga
      541 ggccttcccg ctcgccgacc gcgtcggcca gggctgggcg cacatcgacc ttcagggcga
      601 tgccctccgg ctgtggcagt tcggcaacgg cgtgcgggag cccgcgactg tcggaggcct
      661 tacgcttgag ccctacggca aggacggggc cctggtggtc tgggtcgcca acctcgccat
      721 ccacacgccg gcggggctcc gcgatgcgct ctcgctgatg gcagacagcg actgcccgcc
      781 gccgacggcc gtcggcccgg tgacgcggtt ggccgaggtg cctcggtgct acatgggtat
      841 gtcgctctcg ttcctcgcga agcccgaggg ccaggagctc gtggcagagg tccgcaagct
      901 cgtgccgagc ctgggcgtgg cggccgccgg agcgcagtgg cgacccgagt gggccgaagc
      961 catcgcgcgc gtccgagccg atggcgtcga tgtgcgcgtg cagaacggcg gcatcgctgg
     1021 tatggcgggc ctcatcaccc gcgcccgagc gtggggcgtc gatccccagg gccgctcgcg
     1081 caacacgacg ccgggctatg gcgacggcat gcacagcgtg agctactgcc atccggctgc
     1141 ccgtgaggcc gtccggcgga tgatggcagc catgcgaggc atgggcatcc tcgactacga
     1201 gcagatcgac tacgtatggc cctggcaggg cggtccctgg ggctatggcg agtgggaccg
     1261 cgaagccttc cgcaccgacc tgctagggca agatgagggc ctgcggctgg cttccggcga
     1321 gacggtgcac ttctccgact acctcgccgc ctatggagtg gaccggatcg gtcctggcga
     1381 cctcgggatc gccacctggg gcttgttcga gccgaaggca gccgagggcc tgccggagga
     1441 cgctcgccgt cgccactcct tcgtgttcac cgcgctcacg cactacgagt ggctgaagtt
     1501 cgcgcaagca atgggggccg aggcccgctc ctggggcggc gagttcctgg cgacgctgaa
     1561 ccccgaggac gtgtcgaatg gcggcgacta cgtgttctgg agccgcctcc gggataccgg
     1621 ggcggagtac ctcgagcgct tcggcaatcc gggcgagacc gagttctggc ggcactacct
     1681 gccctatctc cgtcaggagg ccacacgctc gggcaagcgc ttgggcgtca tctacgaggt
     1741 aggcgtgggc ggccatggcc agccctacca cgaccccgag gtcgcctact gcctctcgta
     1801 cgactgcacg gccagcggcg ggttcgacga catgcagaac gagtggatgg atgaggccgg
     1861 ctgggccgcc tcgcaggagg ggaggcatca cgaccgactc cagcaatggc tcgccacggc
     1921 ccatgcattc aaccaggcca aggccgatgg agcggcgctc cccgcgcggc gggccctggc
     1981 catcgggcag cgctgcgtgg tgcacgcgat cgggttcagc ccgtcgtatc tcctgacgcg
     2041 gggcgcgctc aacgacctgg gcatcgactg cgactattcc gacctcacca tggacccggc
     2101 cgactgggag gcctacgacg tgctcgtgta cacgccgtgg gagtcctccc accggcacct
     2161 tgaggccctg cctcggtggc tggacgcgaa gcccggccgg atggttatca cgcatggcgc
     2221 cgtgccgacg cggatcgcca gtgggctcgg gtacgcgccg agcgcggcgg taggcgaccc
     2281 ggggttgggc gggctgctcg gacttggcga gatcacggtc ggcggggcag ccgagggcgt
     2341 tgtgagccag gtgagtcctg gtctggcggc tgcattggcg cccgggcagg cgccgtcact
     2401 acctggcggc ctgtaccaga ctgcccaagg cgaggccgtt gcccgcatcg gcgacgccgc
     2461 gctcgtcacc cgggccgccc ggcctaacgg aagcgtcgtc tactacctca actacgcggc
     2521 c
//
LOCUS       DAOSPL010000642         2518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_41303_length_2518_cov_3.903776, whole genome shotgun sequence.
ACCESSION   DAOSPL010000642 DAOSPL010000000
VERSION     DAOSPL010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41303_length_2518_cov_3.903776"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PLQ54_11820"
     CDS             complement(<1..351)
                     /locus_tag="PLQ54_11820"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOS93994.1"
                     /translation="MQPQAPREAVERLCRAFDGVVVIDEAYVDFGDDNCMDFPKRFEN
                     AIVMRTFSKSFSLAGMRLGAAVAQPSIIHEFMKTKDSYNLNACGQAAALAALQDYAHM
                     EANVAKVRATRARLR"
     gene            481..1266
                     /locus_tag="PLQ54_11825"
     CDS             481..1266
                     /locus_tag="PLQ54_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93995.1"
                     /translation="MPPEPTAEGAAPPRRRRWRFRLALVLVIVGCAGAGCASYLGLRW
                     LAQHEPKPTYFQAEALPAPPPKVNQGPQVLPREALTFVAQVHTKPDKNAPIERYRGVE
                     LADVGLAVVDARFIDSASLPPLDGNAAYYAVELAATTTRKEPVRIADVAVDLQGSGLE
                     MQQQPPEFVDVDEGYTVIPKDAKTVTFRAVFAIPKTAANLAVSLGVWAPDGRIAQAVV
                     PAEPVPDRGPQPTARSTPGTGKTPDAGDLPSREVVEQMEPPTD"
     gene            complement(1263..2297)
                     /locus_tag="PLQ54_11830"
     CDS             complement(1263..2297)
                     /locus_tag="PLQ54_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93996.1"
                     /translation="MIRRHTLAGWAVALVMATAGASFGYVRGNYEYHFWHGVQHLVAH
                     AGSRGQAEIQYGLRQLRYCRAAAVRDTKKVAARAQKARQDGDTALADACQLVIDFHER
                     LRLPEADATEARQRELLALIQPAEGEPPQALIDAIGYAQSHEDEARVVEVNAIFAEQR
                     LKDFAQGYALRRQAVDTLTRVEAALRARSAEVETACPEAAGYLRQHADETAARARYLE
                     RQYQLCSDPEGMARRYAFYAKVAQAHQGLQAEIWSIIRDTYRGKTLFAIDLARAAGGD
                     TTALDDLARRIADNDLAGLEPSMPEWYYLIEMELPFFHHYGAGKEFGGTTEIANELLR
                     VHESGDDNPR"
BASE COUNT          403 a          872 c          819 g          424 t
ORIGIN      
        1 gcgaagacgc gcccgcgttg cgcggacctt ggccacattg gcctccatat gggcatagtc
       61 ctggagggcc gccaacgccg ccgcctggcc gcaggcgttc aggttgtacg aatccttggt
      121 tttcatgaat tcatgaatga tcgagggttg tgccaccgcc gcgcccagcc gcatgccggc
      181 caggctgaac gacttggaga aggtccgcat tacgatggca ttttcaaacc gtttggggaa
      241 atccatgcag ttgtcatcgc caaaatccac gtaggcttcg tcaatcacca ccacgccatc
      301 aaatgcacgg cacaaccgtt cgacggcttc gcggggcgcc tggggctgca cccggcttcg
      361 ggggcctggc catggctggg ctcgttcgac gaggtgcgga tctacgaccg ggcactgacc
      421 caggaggagg tgggccgcct ggcggcgcgc taggtggaac cgacgtcgac agctgagtct
      481 gtgccacctg aaccgacggc cgagggcgca gcccccccac ggcggcgccg ttggcgattc
      541 cgcctcgcac tggtgctggt catcgtgggg tgtgccgggg ccggttgcgc atcgtacctc
      601 ggcctgcgat ggctggctca gcacgagccc aaacccacct acttccaggc cgaggcactc
      661 cccgcacccc cgcccaaggt gaaccaggga ccccaggtcc tgccgcgcga ggccctcaca
      721 ttcgtggcgc aggtgcacac gaagccagac aagaacgcgc ccatcgagcg gtaccgcggt
      781 gttgagctcg ccgacgtggg tctggccgtt gtcgacgcgc ggttcatcga ctccgcctcg
      841 ctgcccccac tcgatgggaa tgcggcctac tacgctgtgg agctggcggc gaccaccacg
      901 cgcaaggagc ccgttcggat cgccgacgtg gctgttgacc tgcagggctc ggggctcgag
      961 atgcagcagc agccgcccga gtttgtggac gtggatgagg ggtacacggt catccccaag
     1021 gacgcgaaga ccgtcacctt tcgcgccgtg ttcgccatcc cgaagaccgc cgccaacctg
     1081 gccgtctcgc taggtgtctg ggccccggat ggccgcatcg cccaagccgt ggtgcccgcc
     1141 gaacctgttc cggatagagg gcctcagccc acggcccggt ctactccggg caccggcaag
     1201 acaccggatg ccggagactt gccaagccga gaggtcgtcg agcaaatgga gccgccaacc
     1261 gactagcgcg ggttgtcatc ccctgactca tgcacccgca acaactcgtt ggcgatctct
     1321 gtggtcccgc cgaactcctt gcccgcacca tagtggtgga agaacggcag ctccatctcg
     1381 atcaggtagt accactcggg catcgacggc tcgagacccg ccaggtcgtt gtccgcgata
     1441 cgccgcgcca gatcgtccag cgccgtcgtg tcgccgcccg ccgctcgagc cagatcgatg
     1501 gcgaacagcg tcttgccgcg gtacgtatcg cgtatgatgc tccagatctc cgcctgcaag
     1561 ccctggtgtg cctgggcgac cttcgcgtag aacgcgtacc gccgcgccat gccctcagga
     1621 tcggagcaga gctggtactg acgctccagg taccgggccc gcgcggccgt ctcgtcggca
     1681 tgttggcgca ggtagccggc tgcctcggga caggcagtct cgacctcggc cgaccgggca
     1741 cggagtgcgg cctccacacg cgtaagggtg tcgaccgcct ggcgtcgcag tgcgtagccc
     1801 tgggcgaagt ccttcagcct ctgctcggcg aagatggcat tgacttcgac cacccgcgcc
     1861 tcgtcctcgt gcgactgggc atagccgatg gcgtcgatca gcgcctgcgg cggctcgcct
     1921 tcggccggct ggatcagtgc gagaagctca cgctggcgcg cctcggtggc atccgcctcg
     1981 ggcaaccgca ggcgttcgtg aaagtcgatc acgagttggc atgcgtctgc gagtgcggtg
     2041 tcgccgtctt gccgcgcctt ttgggcgcgt gccgccacct tcttcgtgtc ccgaacggca
     2101 gcagcccgac agtagcggag ctgccgcaga ccgtactgga tctcggcctg gccacggctt
     2161 ccggcatggg cgaccaggtg ctgcacgccg tgccagaagt gatactcgta gttgccccgg
     2221 acgtagccga agctcgcacc ggccgtggcc atcaccaggg ccactgccca accggcgagt
     2281 gtatgccgtc tgatcacagc gggactccac atcgtacgcg agccgtgcgg gctcgggtaa
     2341 ccatcgttgg ggaaccgagg gcacattcta gcagcacgcc accgcgaccg gaagcgcggc
     2401 tctggccctc cggaggcctc tcttggttct tccgccgggc cgccaacctc ctgtggcacc
     2461 gatggcctcg ggcccaatgg agtcccgccc gcctgccgag ggtctcgtgt gcgggccg
//
LOCUS       DAOSPL010000643         2517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_41329_length_2517_cov_3.474005, whole genome shotgun sequence.
ACCESSION   DAOSPL010000643 DAOSPL010000000
VERSION     DAOSPL010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41329_length_2517_cov_3.474005"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..1423
                     /locus_tag="PLQ54_11835"
     CDS             56..1423
                     /locus_tag="PLQ54_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015300237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOS93997.1"
                     /translation="MARRAPNIVLIICHDLGQYCRCYGADAPTPHVDELAARGVLFSN
                     YHCSAAQCSPSRGSIMTGRMPHTNGLAGLAHLGFGYHPGERTLAMYLAEAGYETVLFG
                     MQHETTADPATLGYSAVHGEPGRARDVMAKAAASLLSRGGDTARPFFYCVGTFEVHRP
                     YHQAGYHHSDPVQVSVPPWLPDRPGIREDIAGLNGLVRELDGAVGLLSRALEQSGLAE
                     ETLVVFTTDHGLAMPRAKGTCFDAGMKTALIVSQPGAVPCGRVDNSLLANCDLLPTLL
                     ELAGVEPPDHLNGRSFAGLLTGRAHEPRHDVLLEMTWHDLYNPMRAVKTANRKYIRSF
                     ARLPLVYLPKDIWQSPSGREVWPEYYAEARPEEELYDLETDPMEYRNVADDPAYAADL
                     ARLRQRVLSEMDRTGDRLLQGPWPAPPRYIEAATGWSRSPSDREWFEQATNGDLACLM
                     PRILR"
     gene            1612..>2517
                     /locus_tag="PLQ54_11840"
     CDS             1612..>2517
                     /locus_tag="PLQ54_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS93998.1"
                     /translation="MAVADSMTPAGSDSLQPGQPAGAPDTLTHPFEGRILTWRSVLLC
                     LVSGFVTVLWIRQVELIKMTCQVSESVPPIPAVAVLVLGAVLLSAWRGGLRKILARRV
                     AVSGAGVVVLAAGMSIYTQEALWCRSVGTLLGLAGLTAAGVGIAAPWLYRARDRLMLT
                     RAEIFGIYIFVSIVSVMSSVGGLRLVLPCLSSLRYFQTTENHFGDFEQYLPDWFGPKD
                     PEAIRQFYEGVNYEAPTPDLGQIPVISEVLGGLASVLGTHRMVPWEHWRAPIAMWFMF
                     LFVFFTTMLCTVALLRRQWADSERLS"
BASE COUNT          406 a          859 c          827 g          425 t
ORIGIN      
        1 ggcctcgcat gccggggcct gggataggcc ggcgcgagcg gaggagcgcg gtcccatggc
       61 acgccgtgcg cccaatatcg tcctgatcat ctgccacgat ctggggcagt actgccgctg
      121 ctacggagcc gatgctccca cgccccatgt cgacgaactc gcggcgcgtg gcgtcctgtt
      181 ctcgaactac cactgctcgg ccgcgcagtg ctcacccagc cggggaagca tcatgacggg
      241 ccgcatgccc cacaccaacg gcctcgccgg cctcgcgcat ctggggttcg ggtaccaccc
      301 cggcgaacgc actctcgcga tgtatctggc cgaggccggg tatgagaccg tgctgttcgg
      361 catgcaacac gagaccacgg cagaccctgc cacactcggc tactccgccg tccacggaga
      421 gcccggacgg gccagggacg tgatggcaaa agccgcagcc tctctcctgt cccgaggagg
      481 cgacacggcc cgccccttct tctactgcgt cggaacgttt gaggtgcacc gaccctacca
      541 ccaggccggc tatcaccaca gcgatccggt gcaggtgtca gtgccaccgt ggctgccaga
      601 ccgccccggg atccgcgaag acatcgcggg cctgaatggc ctggtgcggg agctcgatgg
      661 ggccgttggt ctgctctcgc gggctctgga acagtcgggc ctcgctgagg agaccctcgt
      721 ggtattcacc acggaccatg ggctggccat gccgcgggcg aagggcacgt gcttcgatgc
      781 gggaatgaag acagcactca tcgtctcgca accgggcgcc gtgccgtgcg gccgggtgga
      841 taactcactt cttgccaact gcgacctgct gccaacactg ctcgaactgg cgggagtcga
      901 gccgccggac cacctgaacg ggcgcagctt cgcgggcctg ttgacaggga gggctcacga
      961 gccccggcac gacgtactgc tcgagatgac gtggcatgac ctgtacaacc ccatgcgcgc
     1021 cgtgaagacg gcgaacagga agtacatccg cagcttcgcg cggctcccgc tcgtctatct
     1081 ccccaaggac atctggcaga gcccgtccgg gcgcgaggta tggcccgagt actatgcgga
     1141 ggcgcggccc gaggaggaac tatacgacct cgagacagac cccatggagt accgaaacgt
     1201 cgccgacgat cctgcctacg ccgcagacct ggcccgactc cgccagcggg tgctctcgga
     1261 gatggaccgc accggcgacc gcctgctcca ggggccctgg ccggccccgc cccggtacat
     1321 tgaggcggcg acgggatgga gtcgttcgcc aagtgaccgg gagtggttcg aacaagccac
     1381 taacggcgat ctcgcctgcc tgatgccccg tatcctccgc taggggccga tggtcgcgcg
     1441 aggacatggc agacgccgct aggttggcgc gggttggccc cggcaccgat gtgtgtcgcc
     1501 acagcgagtg catggtccgc cagcccgcac cagagtcaac caggattcgc ctatgccgag
     1561 ggtgaactgg cccccggcca tggcgaacag acccggggta gaggaggaag gatggcagtc
     1621 gccgacagca tgaccccagc cggcagtgac agcctccagc ccggtcagcc ggccggtgcg
     1681 cccgataccc tcacccaccc gttcgagggg cgcatcctga catggcggag cgtgctcctc
     1741 tgcttggtct caggcttcgt cacggtcctg tggatccggc aggtcgagct catcaagatg
     1801 acctgccagg tgtccgagag tgtccctcca atccccgcag tggccgttct ggtgcttgga
     1861 gccgtgctcc tgtccgcctg gcgcggcggg ctgcggaaga tcctcgcgcg gcgtgtggcc
     1921 gtgtccggcg cgggtgtcgt ggtgctggcc gccggcatga gcatctacac gcaggaggcc
     1981 ttgtggtgcc gttcggtggg gacactactg ggcctcgccg gcctgacggc ggcgggcgtg
     2041 ggcatcgcgg ccccctggct ataccgggcg cgtgaccgcc tgatgctgac ccgagccgag
     2101 atcttcggga tctacatctt cgtgtcgatc gtctcggtca tgtcctcggt gggtgggctt
     2161 cgactcgtgc tgccgtgtct ttcatccctc cggtacttcc agaccaccga gaaccacttc
     2221 ggcgacttcg agcagtacct ccccgactgg ttcggtccca aggaccccga ggccatccgg
     2281 cagttctatg aaggagtcaa ctacgaagcg cccacaccgg acctcggcca gatcccggtg
     2341 atcagcgagg tgctgggcgg cctggcctcg gttctgggca cccaccgcat ggtcccatgg
     2401 gagcattggc gtgcgcccat cgccatgtgg ttcatgttcc ttttcgtctt cttcacgacc
     2461 atgttgtgca cggtggctct gctccgcaga cagtgggccg atagcgagcg cctgagc
//
LOCUS       DAOSPL010000644         2515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_41385_length_2515_cov_2.980894, whole genome shotgun sequence.
ACCESSION   DAOSPL010000644 DAOSPL010000000
VERSION     DAOSPL010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41385_length_2515_cov_2.980894"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLQ54_11845"
     CDS             <1..210
                     /locus_tag="PLQ54_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HOS93999.1"
                     /translation="VEATLSAFIRRGGHIFQGNTSDFERLEDALVHPERHRDLMVRVG
                     GFSARFVNLSPVIQQEIVARRRYAG"
     gene            complement(298..1674)
                     /locus_tag="PLQ54_11850"
     CDS             complement(298..1674)
                     /locus_tag="PLQ54_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOS94000.1"
                     /translation="MSHRMSRREALKAMGLGAAALGVPRSAEAIPPARRPNIIFILAD
                     DLGYGDVGCYGQERIQTPRIDQLAREGVRFTDCYSGSTVCAPSRCCLMTGLHTGHARI
                     RGNADVPLRPEDQTVAETLKAAGYATGIVGKWGLGNAGTTGIPNRQGFDEWFGYLDQT
                     LAHNYYPPTLWRNEREVPLLGNQNGATNEYSHDLFTREAEAFIRRHAGGPFFLYLAYT
                     IPHANNELGAKTGNGMEVPSDVPYTDQPWPQPEKNKAAMITRMDRDIGHLMDVLEELD
                     IEEDTIVFFSSDNGPHKEGGIAPAFFGSSGPLRGIKRAMYDGGIRVPMIVRWPGRTPA
                     GAVSDYPWAFWDFLPTAAQLGGAPIPKGLDGLSVVPALLGQPGPEREYLYWEFHEGGF
                     SQALRMGRWKAVRNSRRSAPIELYDITTDIGEQTDVSTVHPELVAKAEKLFVSARTDS
                     PEFAVKEE"
     gene            complement(1915..>2515)
                     /locus_tag="PLQ54_11855"
     CDS             complement(1915..>2515)
                     /locus_tag="PLQ54_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94001.1"
                     /translation="IIVYEDRLTGCISEPRIFEDWRAEVEQANELFHPAAFFMSHDEM
                     RVMNQCALCQSKGMTPGELLAWNVRQAAQIIRDARPDAEIWVWSDMFDPMHNAVDHYY
                     AVNGSLAGSWKGLDPDVGIVNWHGGLKGKNCPFFADLGLRQILSGYYDGDEDGSAIAE
                     WIDATKDVKGIVGAMYTTWEDKYGAMDTWAQKAWGGGGK"
BASE COUNT          373 a          866 c          824 g          452 t
ORIGIN      
        1 gtcgaagcca cattgagcgc attcatccgc cgcggtggcc acatcttcca gggcaacacc
       61 agcgacttcg agcggctcga agatgccctg gtgcaccccg agcggcatcg cgatctgatg
      121 gtgcgcgtgg gcgggttctc ggcccgattc gtgaacctat cgcccgtgat ccagcaggag
      181 atcgtggccc gaagaaggta tgcggggtag ggttgtcgtg tgcatgcccg ggggcctgaa
      241 cggcaggtcc cgctcacgac cttctccgcg cacggggaag tgagaccctg tgtcccccta
      301 ctcctccttc accgcgaact caggcgagtc ggtccgggca ctcacgaata gcttctcggc
      361 cttcgctacc agctccgggt gcaccgtgga gacgtcggtc tgctcgccga tgtcggtggt
      421 gatgtcgtag agctcgatgg gcgcgctgcg cctgctgttg cggacggcct tccaccgccc
      481 catccgcaac gcctggctga agccgccctc gtggaactcc caatagaggt actcgcgctc
      541 gggccccggc tggccgagca gcgcgggaac gacggagaga ccgtcgaggc ccttcgggat
      601 cggcgcccca ccgagctgcg ccgcggtagg gaggaagtcc cagaaggccc acgggtagtc
      661 gctcaccgcg cccgctggcg tccgccccgg ccagcggacg atcatgggca cacggatccc
      721 gccatcgtac atcgcccgtt tgatgccgcg cagcgggccg gagctgccga agaacgccgg
      781 agcgatgccg ccttccttgt ggggaccgtt gtcgctgctg aagaagacga tcgtgtcctc
      841 ctcgatgtcg agctcctcga gcacgtccat caggtggcca atgtcgcgat ccatccgcgt
      901 gatcatggcc gccttgttct tctccggctg gggccagggc tgatcggtgt atggcacatc
      961 gctgggcacc tccatgccat tcccggtctt tgcgcccagc tcgttgttcg cgtgcgggat
     1021 ggtgtacgcc aggtacagga agaacgggcc gccggcgtgc cggcggatga atgcttcggc
     1081 ctctcgggtg aacaggtcgt ggctgtattc gttcgtggcg ccgttctggt tgcccagcag
     1141 cggtacctca cgctcgttgc gccaaagcgt tggcgggtag tagttgtggg ccagcgtctg
     1201 gtccagatag ccgaaccact cgtcgaagcc ctggcggttc ggaatgccgg ttgtgcccgc
     1261 attgccgagc ccccacttgc cgacgatccc ggtggcgtag ccggccgctt tgagcgtctc
     1321 ggcaaccgtc tggtcctcgg gccgcagggg cacgtcggca ttgccccgga tccgcgcatg
     1381 gccggtgtgc aggcccgtca tcaggcagca gcgcgacggt gcgcacaccg tactccccga
     1441 gtagcagtcg gtgaaccgga cgccctcacg ggcgagctgg tcgatacggg gcgtctggat
     1501 ccgttcctgc ccatagcacc ctacatcgcc gtagcccagg tcatcggcca ggatgaagat
     1561 gatgttcggc cggcgggccg gtgggatggc ttccgccgag cggggcactc ccagtgcggc
     1621 agctccaagc cccattgctt tcagcgcctc tctgcggctc atgcggtggc tcatcggcct
     1681 catctcctgc tagcaggata cccaaacccc tggccgcgcg tacaggggcg ggatggtggg
     1741 ggagggagtc tggcgccggc ggacctcgaa cccgccgacc gtgagccgcc gcgtgcacgc
     1801 ggcgtgccga agggcggcga gcgcagcggc ctcctcttcc ccttcgtgag gcgccccgcc
     1861 ttggcccacc acgcgctcct cagggccgac ggcaaagggg acaacgcccg ccccctactt
     1921 gcctccgccg ccccaagcct tctgggccca ggtgtccatg gcgccgtact tgtcctccca
     1981 cgtggtgtac atggcgccca cgatgccctt cacgtccttg gtcgcgtcga tccactcggc
     2041 gatggccgag ccatcctcgt cgccgtcgta gtagcccgac aggatctgcc gcaggccgag
     2101 atcggcgaag aatgggcagt tcttgccctt gagcccgcca tgccagttca cgatgcccac
     2161 atcggggtcc aggcccttcc acgagccggc gagcgagccg ttcacggcgt agtagtggtc
     2221 caccgcgttg tgcatcgggt cgaacatgtc gctccagacc cagatctcgg catcgggccg
     2281 cgcgtcgcgg atgatctgcg cggcctggcg tacgttccac gccaggagct cgccgggtgt
     2341 catccccttg ctctggcaca gcgcgcactg gttcatgacc cgcatctcgt cgtgcgacat
     2401 gaagaacgcc gcggggtgga acagctcgtt ggcctgctcg acctcggccc gccagtcctc
     2461 gaagatcctc ggctcgctga tgcaccccgt caatcggtcc tcgtagacga tgatg
//
LOCUS       DAOSPL010000645         2513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_41430_length_2513_cov_2.916192, whole genome shotgun sequence.
ACCESSION   DAOSPL010000645 DAOSPL010000000
VERSION     DAOSPL010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41430_length_2513_cov_2.916192"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1794
                     /locus_tag="PLQ54_11860"
     CDS             <1..1794
                     /locus_tag="PLQ54_11860"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HOS94002.1"
                     /translation="WNSPEVLAAHWRLRDAPAGPERTEPSPMGETHNAALCVPIMLEP
                     GEAKSTTFTLAWHFPNVQRFDHFGNYYTRRFTSARAVAEYVAERLHGLWNRTELYHQT
                     LYQSNLPEEFIDAMSSQSVIFRGPTCWWSEEGYFAGYEGCYGCCPLNCTHVWNYAQTH
                     ARLFPEIGRNLRVSDLITYIHPTGETSHRQHSPHGAFIDGHCAVIEATLREHQLSPDI
                     SFLRRVWPNVRKAVDWLIEAIDKDHDGVPAGHQWNTYDCAVSGPNTFIGSQYLSALAA
                     GEKMAEAMGAPAIAARWRAIRMAGMANQDAQLWNGEYYIQIPENPPAHDYNGGCHSDQ
                     LLGQWWAHMLNLGYLFPRHRVRQALQSVMKYNMKDNFVGFTQAPRRYVPDDEGGLLMC
                     TWPKGGRPDPFTIYSDEVWTGIEYATAGLMVYEGMIDEARRIVRTARSRYDGRLRPEL
                     NSGPGGNPFNELECGKFYARAMSSWSLLIACQGMVLDGPAGLIGFKPRWRPEDHRSFY
                     TAPEGWGLFVQYREARRQVSRIDVRHGRLRVRELVVQAPPVAPAMRVHVRAGGRRVRV
                     TASQQADEVRIRCPKWMTIPEGQEVEVELTW"
     gene            2208..>2513
                     /locus_tag="PLQ54_11865"
     CDS             2208..>2513
                     /locus_tag="PLQ54_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94003.1"
                     /translation="MVLGGIMIGFAAIGLGLAVINLLSASMTSAMMKANPEVAQAMKD
                     LPVQGWTVFQALLGIFGALVLLPAGVGVCMAKSWGRVLALGWSGYHIIMVIPKIVFGM
                     "
BASE COUNT          408 a          864 c          814 g          427 t
ORIGIN      
        1 tggaatagcc ccgaggtctt ggccgctcac tggcggctca gggacgctcc cgcgggtccc
       61 gaacgtaccg aaccaagccc catgggcgag acacacaacg ccgccctctg tgtgcccatc
      121 atgctggagc cgggcgaggc gaagagcacc acgttcacct tggcctggca tttcccgaac
      181 gtgcagcggt tcgaccactt cgggaactac tacacccgcc ggttcacatc ggcccgggct
      241 gtggccgagt acgtggctga gcgtctccat ggcctctgga accgcacgga gctctaccac
      301 cagacgctct atcagtcgaa cctgcctgag gagttcatcg atgctatgag ctcccagagc
      361 gtcatcttcc gagggccgac gtgctggtgg agtgaggagg gctacttcgc cggctacgag
      421 ggctgctacg gatgctgccc cctgaactgc acccatgtct ggaactatgc ccagactcac
      481 gctcgcctgt tccctgagat cggccgaaac ctacgggtgt ccgacctgat cacctacatc
      541 caccccacgg gtgagacctc gcaccggcag cacagccccc atggggcatt catcgatggc
      601 cactgcgccg tcatcgaggc cacactgcgc gagcatcagc tgtcgcccga catctcgttc
      661 ctgcgacgcg tgtggcccaa cgtgcgcaag gccgtcgact ggctcatcga ggccatcgac
      721 aaggatcacg atggagtccc cgcgggccac cagtggaata cctacgactg cgccgtctcc
      781 ggcccgaaca cgttcatcgg gtcccagtat ctctcggctc tcgccgcggg cgagaagatg
      841 gccgaggcca tgggcgcgcc cgccatcgcg gcccggtggc gcgccattcg catggccggc
      901 atggcgaatc aggacgcgca actgtggaac ggcgagtact acattcagat ccccgagaac
      961 ccgccggccc acgactacaa cggtgggtgc cacagcgacc aactcctcgg ccagtggtgg
     1021 gcccacatgc tgaacctggg gtacctgttc ccgcgccacc gtgtccggca ggcactccag
     1081 agcgtcatga agtacaacat gaaagacaac ttcgtgggct tcactcaggc gccgcgccgc
     1141 tacgtccccg acgatgaagg cgggctgctg atgtgcactt ggcccaaggg aggccgtccg
     1201 gatccgttca ctatctactc cgatgaggtg tggacgggca tcgaatacgc gacggccggc
     1261 ctgatggtct acgaggggat gatcgacgag gcccggcgta tcgtccgcac cgcgcgctcg
     1321 cgctacgacg gccgcctgcg gcccgagctg aacagcgggc cgggcgggaa ccccttcaac
     1381 gagctcgagt gcggcaagtt ctacgcgcga gccatgagct cgtggtccct gctcatcgcc
     1441 tgccagggga tggtactcga cggcccggcc ggtctcatcg gcttcaagcc ccgatggcgg
     1501 cccgaggacc atcgctcgtt ctacaccgcc cccgagggct gggggctgtt cgttcaatac
     1561 cgcgaggccc gccgccaggt ctctcggatc gatgtgcgcc acggccgcct ccgcgtgcgg
     1621 gagcttgtcg tgcaggctcc gccggtcgcc cccgcgatgc gtgtccacgt ccgcgccggg
     1681 ggccgccgcg tccgcgtcac cgcatcgcag caggccgacg aggtgcggat ccgctgcccg
     1741 aagtggatga ccattccgga ggggcaggag gtcgaggtcg agctcacgtg gtaagtccga
     1801 ccggaggcat gcggtagcca gggcggatgg cgaatgccgg cggcgcttgg gccctcgcga
     1861 cgttcaaaac gattcgcgga acaccgggcc ctgtccgggc gtacgcctga agtgccgaga
     1921 ggcagcaagt tcgtggccgc cgatgcggca gagggagggc tactgtgttg actctgtgtc
     1981 cggtctgcgg agccaggctt ccccgagggc tccatgtgtg cacccagtgt cgcggctcgc
     2041 tcttggacga ccggggaagc gatgtgcgtc cgccggggcc cccgagcgtg cagccgccgg
     2101 gtgggggcgt gttccagccc gctgggccgc cccagccacc accgggtttc accgggccct
     2161 cgggcccgcc tttatcctac ggccctcccc ccaagcccac gagcctcgtg gtcctcggcg
     2221 gcatcatgat cggcttcgcc gcgatcggcc tcggcttggc cgtcatcaac cttctgtctg
     2281 cctccatgac gagcgccatg atgaaggcga accctgaggt cgcccaggcc atgaaagacc
     2341 tccccgtcca gggctggacc gtgttccaag ccctcttggg cattttcggc gcactggtcc
     2401 tgctgcccgc cggcgttggc gtgtgcatgg ccaagtcgtg ggggcgtgtt ttggccctcg
     2461 ggtggagcgg ttaccatatc atcatggtta tcccgaagat cgtgttcggg atg
//
LOCUS       DAOSPL010000646         2510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_41491_length_2510_cov_3.207332, whole genome shotgun sequence.
ACCESSION   DAOSPL010000646 DAOSPL010000000
VERSION     DAOSPL010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41491_length_2510_cov_3.207332"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLQ54_11870"
     CDS             <1..304
                     /locus_tag="PLQ54_11870"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOS94004.1"
                     /translation="WGGTSDHGLDCSGFVQRVFAEEGIRLPRVAVDQAAVGMAVDRGA
                     LQPGDRVYFRSGSEIDHTGIYIGEGRFIHSTRSGGGVIVSELSSPKWTRIYAGARR"
     gene            423..2363
                     /gene="metG"
                     /locus_tag="PLQ54_11875"
     CDS             423..2363
                     /gene="metG"
                     /locus_tag="PLQ54_11875"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOS94005.1"
                     /translation="MPSGTPKPFYITTPIYYINALPHIGTAYTTIVADTVARFQRYMG
                     REVLFATGTDEHAEKVVQAAKARGMTAEAYSAEMADKFREAWCRLDITFDDFIRTPEP
                     RHHRAVQELVRRLQESGDIYAGDYEGWYCVPCATFFLENDLEEGNCPDCGREVERRSQ
                     PAYFFAASKYREPLLKHIEEHPDFIVPDFRRNETVSFIREMRDACISRPNDGWGIPFP
                     NDPAHVVYVWFDALINYITVAGWPDDLERFERLWPADLHLMAKDILPRFHGSMWPAML
                     MALGLPLPKKVAAHGYWQMGGAKISKSRGNVINPFELASDLEKETGIDFNVALDAIRY
                     FVLRETAFGQDAGFTDEAFRGRLNADLANDLGNSLNRCLPLIERNCEGRVPEGTLDPA
                     LAAEVESAAATARAAFEDLDFRGALEAVWAVIAQINRYVGTTEPWALARDPGSAQKLA
                     DVLYTAAECIRCVTAMLYPVMPHAVAAMRVQLGLSPAPEGTWNQAVAPGAEGLAGRPV
                     ARGNPVFPRVELRPAEPPEPVEREATTVDTVTYEDFMKIQLRVATVESGERVEGADKL
                     YKLNLTLGDEKRTIVAGLAQDFGLDELVGKQIIIVANLEPRKVRGVLSQGMLLAVTDP
                     VTDRPVAIVTVDRPAPSGARLR"
BASE COUNT          412 a          819 c          852 g          427 t
ORIGIN      
        1 ctggggaggg acgagtgacc acgggctcga ctgctcgggc ttcgtgcagc gcgtgttcgc
       61 cgaggagggc atccggctgc cgcgcgtggc cgtcgaccag gccgccgtgg ggatggcggt
      121 ggatcgcggc gcgcttcagc ccggtgaccg cgtgtacttt cggagtggga gcgagatcga
      181 tcacacgggc atctatatcg gcgagggccg ctttatccac tcgactcgaa gcggcggcgg
      241 tgtcatcgtg agcgaactct catcgccgaa gtggacgcgg atctacgcag gggcccggcg
      301 ctagcgcggg aacgccgcgt ccgcgggcat tgtttgtagc cctcgagtct atgccgctat
      361 actgctggca cacccgcaga tctcacgtcc ccgtggcgct ttcgggtgcc gaggagttcg
      421 cgatgccatc cggtacgccc aagcctttct acatcacgac accgatctac tacatcaacg
      481 ccttgccgca catcggcacg gcctacacca ccatcgtggc cgacaccgtg gcgcgctttc
      541 agcgctacat gggccgagag gtgctgttcg ccaccggcac cgacgagcac gcggagaagg
      601 tggttcaagc ggccaaggcc cgcggcatga ccgccgaggc ctattccgcc gagatggccg
      661 acaagttccg cgaggcctgg tgccggctcg acatcacttt cgacgacttc atccgcactc
      721 ccgagccgcg ccaccaccgg gcggtgcagg agctcgtgcg ccggctgcag gaaagcggcg
      781 acatctacgc cggtgactat gagggctggt actgcgttcc gtgtgcgacc ttcttcctcg
      841 agaacgacct cgaagagggc aactgccctg actgcggccg tgaggtggag cgccggtcac
      901 agcccgccta cttcttcgcg gcgtcgaagt accgcgagcc gttgctcaaa cacatcgagg
      961 agcacccgga cttcatcgtc cctgacttcc ggcgtaacga gacggtgtcg ttcatccgcg
     1021 agatgcgcga cgcgtgcatc tcgcggccca acgacggttg ggggatcccg tttccgaacg
     1081 atcccgccca cgtggtctat gtatggtttg atgcgctcat caactacatc acggtggccg
     1141 gctggccgga tgatctggag cgcttcgagc gtctgtggcc tgcggatctg catctcatgg
     1201 ccaaagacat cctgccgcgc ttccacggca gcatgtggcc ggcgatgctg atggctctgg
     1261 gcctcccgct gccgaagaag gtcgccgcgc acggctactg gcagatgggc ggggcgaaga
     1321 tctcgaagtc gcgcggtaac gtgatcaacc ccttcgagct ggcctccgac ctcgagaagg
     1381 agaccggcat cgacttcaac gtcgcgctcg atgccatccg ctacttcgtg cttcgcgaga
     1441 ccgccttcgg gcaagacgcc ggcttcaccg acgaggcgtt tcgcggccgc ctcaacgccg
     1501 acctggccaa cgacctgggg aactcgctca accgctgtct gccactcatc gagcgcaact
     1561 gcgaggggcg cgttcccgag ggaacgctcg acccggcact ggcggcggag gtggagtccg
     1621 ccgcagcgac ggcccgggcg gcattcgagg atctcgactt ccggggcgcg ctcgaggccg
     1681 tctgggccgt catcgcgcag atcaaccgct acgtcggaac aacggagccc tgggcgctgg
     1741 cgcgagaccc cggatcggcc cagaagctgg cggacgtgct ctataccgcg gcggagtgta
     1801 tccgctgcgt caccgccatg ctctacccgg tgatgcccca cgccgtggcg gcgatgaggg
     1861 tgcagctcgg gctctcgccc gcgccggagg gcacctggaa tcaggcggtg gcgccgggcg
     1921 cggaagggct ggcgggccga cccgtcgccc ggggcaaccc ggtgttccca cgcgtcgaac
     1981 tcaggcccgc tgaaccgccc gagcccgttg agagggaggc cacaacggtg gatacggtca
     2041 cctatgagga cttcatgaag atacagctac gggtcgccac cgttgagtcg ggcgagcggg
     2101 tcgagggtgc cgacaagctc tacaagctga atctcaccct cggcgatgag aagcgcacga
     2161 ttgtggcggg actggctcag gacttcgggc tcgacgagct ggtcggtaag cagattatca
     2221 tcgtggcgaa tctcgaacct cggaaggttc gtggcgtgct ttcccaggga atgctgctgg
     2281 cagttaccga tcccgtcacc gatcggccgg tcgcgatcgt cacggtggat cggccggcgc
     2341 catcaggcgc tcggctacgc tagcctcgaa cgctcaccgg gagggcgccg gatgcgcggg
     2401 tggttgctcg cacgggcatt cgtcgctgtg gcgcttggtc tgccgatcgg gtccggctgc
     2461 cgaatgcggc gctgacggga agcgtggctt cccgacgggt tggtcaggag
//
LOCUS       DAOSPL010000647         2509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_41508_length_2509_cov_3.681337, whole genome shotgun sequence.
ACCESSION   DAOSPL010000647 DAOSPL010000000
VERSION     DAOSPL010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41508_length_2509_cov_3.681337"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLQ54_11880"
     CDS             <1..405
                     /locus_tag="PLQ54_11880"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOS94006.1"
                     /translation="GELLATLEETRIPTLIEMDQTSWDELDDVARRHPDLRLVALRVG
                     YRADRMMYPVLERRARIFIETSNYVGHRAIEHFVDTFGAGRLVFGTAAPVFAPGGAVA
                     PVLYAEVDEAAKAAIAGGNLRRLIEDSSVGEA"
     gene            408..1220
                     /locus_tag="PLQ54_11885"
     CDS             408..1220
                     /locus_tag="PLQ54_11885"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOS94007.1"
                     /translation="MEARSIEARVLAGEALRDELVVDVHCHMGPWAAFVVHEGGPAGM
                     VRTMDRCGIDWACIAPHAAIGPDFGGGNDEAARARDEIPGRFIAYCTLNGRYPAEANA
                     AELDRCLAMGFRGIKLHPAVHQTPLDGPNLRPAFETASARELPVLVHTWHNDALASPA
                     MFEALCHDFPGIRFILGHTGGSPEGADQYLALAPRCEHVYFDLCGSLMNLGMIERIVD
                     AAGDERVLFGSDLPFIDCRPMLGYIGFSRVSDESKRRILGLNAARLFGLGDR"
     gene            1478..2206
                     /locus_tag="PLQ54_11890"
     CDS             1478..2206
                     /locus_tag="PLQ54_11890"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOS94008.1"
                     /translation="MRIGVISDIHGNLEALSATLEALADVDHVVCLGDAVGYGANPNE
                     CCHRLMTACRTAIRGNHDRAALDESLAEEFNNDARVAAFWTAEVLDRTSWFYLNALPD
                     VAEAGSALLVHGAPSDPDGYVLCAEQAQLEFFLMDAAVCFFGHTHLPEAYGYEASSAV
                     ATPIGLTAGKPLRLASTTRYLLNPGSVGQPRDGDPRASCGVYDEQVGTFELRRVEYNV
                     RRAQEKILRAGLPPFLASRLETGG"
     gene            2211..2432
                     /locus_tag="PLQ54_11895"
     CDS             2211..2432
                     /locus_tag="PLQ54_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94009.1"
                     /translation="MSTIAAIVIGLVLAFPFTWALQGVYLWLSGSQLDASAASATDMR
                     LIIVIVLLCVSLFRARRPTLGPPPPRGAR"
BASE COUNT          374 a          851 c          861 g          423 t
ORIGIN      
        1 ggcgagctcc tggctactct cgaagagact cgaatcccta cactcatcga gatggaccag
       61 acctcatggg acgagctgga cgacgtggcc cggaggcacc ccgacctgcg gctggtggcc
      121 ctgcgcgtcg gctatcgtgc cgaccgcatg atgtaccccg tgctcgaacg ccgggcccgc
      181 atcttcatcg aaaccagcaa ctacgtgggc caccgtgcca tcgagcactt tgtcgacacc
      241 ttcggcgccg gccggctcgt gttcggcacc gcggcacccg tgttcgcgcc cggcggggct
      301 gtggcaccgg tgctctacgc ggaggtcgac gaggccgcca aggcggccat cgccggcgga
      361 aacctgcggc ggctcattga ggattcgtcc gtgggggagg cttaggtatg gaggcgcgtt
      421 ccatcgaggc tcgagtgctc gcgggcgagg ccttgcgtga cgagctggtg gtcgatgttc
      481 actgccacat gggtccctgg gcggccttcg tggtccatga gggcggcccc gccgggatgg
      541 tgcggaccat ggaccgctgc ggaatcgact gggcctgcat cgccccgcac gccgccatcg
      601 gcccggactt cggcggcggc aacgacgagg cggcgcgagc ccgagacgag attcctggac
      661 gcttcatcgc ctactgcacg ctcaacggac ggtacccggc tgaggcgaat gccgccgagc
      721 tcgatcgctg cttggccatg gggtttcgag ggatcaagct ccacccggcg gtgcaccaga
      781 cacccctgga tggccccaat ctgcgacccg cgttcgagac agccagtgcc cgcgaactgc
      841 ccgtcctggt tcacacctgg cacaacgacg cgctcgcctc gccggccatg ttcgaggctc
      901 tatgccacga cttccccggc atacggttca tcctcggcca caccggaggg tctcccgagg
      961 gggccgacca gtatctcgct ctcgccccaa gatgcgagca tgtctacttc gatctgtgcg
     1021 ggtcgctgat gaacctgggg atgatcgagc gtatcgtcga tgccgcgggc gacgagcggg
     1081 tgcttttcgg ctccgacctg ccattcatcg actgccgtcc catgctggga tacatcggct
     1141 tctcgcgggt gtccgatgag tcgaaacgtc ggatactggg cctcaacgcg gctcgtctgt
     1201 tcgggctggg tgacaggtga ggggcatggg gggcaacttc gggttcgggc ggcccacggg
     1261 ccgggtgggg gggcgccacg cgccctttgt gggaccccga aagcggcgcg aagccccgtt
     1321 cgggtgggct ccgtcggccg tgcggccggc cgggtccctc ggctggcaca ggaccggggt
     1381 ccctctaccg gaccgggcag tgcggtatac gggccttccg cgcaccgccc aggcctgagt
     1441 gaccgccaat gcgggtgagg gggcggagga cccagagatg cggatcggcg tgatctcgga
     1501 catccacggc aacctcgagg ccctgtcggc cacactggag gcgctggccg atgtcgacca
     1561 cgtcgtctgc ctgggcgacg ctgtgggcta cggtgcgaac cccaacgaat gctgccaccg
     1621 gctgatgacc gcgtgccgga cggcgatccg cgggaaccac gaccgggcgg ccctggatga
     1681 gtctcttgcc gaggagttca acaacgacgc gcgagtggcc gcgttctgga ccgcggaggt
     1741 cctcgaccgc acttcgtggt tctacctcaa tgcgctgccc gatgtggccg aggcgggatc
     1801 cgctctgctc gtacacgggg cgccctccga tccggatggc tacgtcctgt gcgccgaaca
     1861 ggcccagctc gagttcttcc tgatggacgc cgccgtatgc ttcttcggac atacccacct
     1921 gcccgaggcc tacggctacg aggcctcgag cgccgtcgcg acccccatcg ggttaaccgc
     1981 gggcaaaccc ttgcgcctgg ccagtacgac ccgctacctg ctgaacccgg gtagtgtcgg
     2041 ccagccccgc gatggggacc cccgagcgtc atgcggcgtg tacgacgagc aggtggggac
     2101 tttcgagctc cgccgggtcg agtacaatgt ccggcgagcc caggagaaga tcctccgggc
     2161 aggcctgccg cccttcctgg cgagccggct ggagactggg ggctgagtcc gtgtcgacca
     2221 tcgccgccat cgtcatcggg ctggtgttgg ctttcccgtt cacctgggcc ttgcagggcg
     2281 tctatctgtg gctctcgggc agccaactgg acgcgtcggc cgccagcgcc accgacatgc
     2341 ggctcatcat cgtcatcgta ttgctgtgtg tgagcctgtt tcgcgcgcgt cggcccacgc
     2401 tgggtccgcc gccgccccga ggcgctcgct gatgggcccg aggaacgatg agcagccgac
     2461 gcgcgatgac gagggacgcc ccgaaggtgc ctccgacctg atcgaacga
//
LOCUS       DAOSPL010000648         2508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_41531_length_2508_cov_3.320016, whole genome shotgun sequence.
ACCESSION   DAOSPL010000648 DAOSPL010000000
VERSION     DAOSPL010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41531_length_2508_cov_3.320016"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2508
                     /locus_tag="PLQ54_11900"
     CDS             <1..>2508
                     /locus_tag="PLQ54_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOS94010.1"
                     /translation="AQYERMDKWQRFILANARYAPKILPFLVKAGFSLARRLGKEKFF
                     AQVNGGSPADMAAFADPERPLTPRITEALGALGIQQLYTHQASAVDAIRRDENVVIVT
                     STASGKTLCYTIPVLESLADDPETRALWMFPTKALAQDQLKTLRRFVEGVPDLPVVTG
                     TYDGDTPQTERRTLRDRGRIILTNPDMLHSGILANHSRWAGFFENLKYVVIDEVHTYR
                     GVFGSHVANVMRRLRRVCTHYGAHPVFVLCSATIANPKELAEAIVGEAVTLVDNDGSP
                     RGRKRFVLWNPPHLDETRLERRSPHTEAERLLVSLIRGGNQTICFVRARVLAETIYRY
                     AQDSLRRHAPRLANAIRAYRGGYIAEQRREIERQLFSGELMGVVSTVALELGIDIGSL
                     DACLTVGYPGSIASLWQQAGRAGRGTEDSLAVLIGYNSPIDQFLMQNPGYLFSQSPEM
                     AVVDPANPHIVAWHLRCACHELPVSAADEAIFGEYLYSVLDILTDSGDIRRRGNRWYW
                     AGQGYPATQFSLRNIPEDVYQIIDQTDGRSETIGETDETSAFFSLYTQAIYMHNAETY
                     FVKELNIDRKHAYIEKVDCDYYTQSVDKREIRVVNADEERKWRVSQVFFGDVTVTEMV
                     VMFRKIQFGARDSIGFGSVDLPPIETDTEALWVVPPRDALDRIRQFGRVPQDGLLGVG
                     NVIVGVMPLFVMSDPMDVGAAVDSSNVGSPSLFIYDKHAGGVGFAQRAYGRIEEIMEA
                     ALSLIHGCDCSDGCPSCVGAPLPPYAYGDVDSSSRGAIPDKEAALCLLHSLLEREPYT
                     PRPVSSPLGEAAAPLPRGPEPTSLTAPAPPPPPDYARL"
BASE COUNT          434 a          814 c          826 g          434 t
ORIGIN      
        1 tgcgcaatac gaacgcatgg acaagtggca gcgattcatt ctggccaacg cccgctatgc
       61 gccgaaaatc ctgccgtttc tggtgaaggc cgggttttct ctggcgcggc ggctgggcaa
      121 agagaagttt tttgcgcagg tgaatggcgg ctcgcccgcc gatatggcgg ccttcgccga
      181 tccggaacgc cccctgacac cgcgcatcac ggaggccctc ggggcactgg gcatccagca
      241 gctctacacg caccaggcct ctgccgtcga cgccattcgc cgcgacgaga atgtggtgat
      301 cgtcacatcg accgccagcg gcaagacgct gtgctacacc attccggtgc tggagtcgct
      361 ggccgatgac cccgagaccc gggccctgtg gatgttcccc accaaagcac tggcgcagga
      421 tcagctcaag acgctgcggc gctttgtgga gggcgtgccc gacctgccgg tcgttaccgg
      481 tacgtacgac ggcgacacac cgcagacgga gcggcgaacg ctgcgcgatc gcggacggat
      541 catcctgacg aaccccgaca tgctgcactc aggcatcctc gcgaaccact cgcgctgggc
      601 gggattcttc gagaacctca agtacgtcgt gatcgacgag gtgcacacgt atcggggcgt
      661 gtttggttcg cacgtcgcca acgtgatgcg gcgcctgcgc cgcgtctgca ctcattatgg
      721 cgcgcacccg gtcttcgtgc tctgcagcgc gaccatagcg aacccgaagg agttggccga
      781 ggccatcgtt ggcgaggccg tgacgctggt cgacaacgat ggctcgccgc gcggccgcaa
      841 gcgcttcgtt ctgtggaacc ctccgcatct ggacgagacg agactggagc gccgaagccc
      901 gcacacggag gccgagcgtc tgctcgtatc gctcattcga ggcggtaacc agacgatctg
      961 tttcgtgcga gcccgcgtgc tggcagagac catctaccgc tatgcccagg acagcctgcg
     1021 gcgccacgcg ccgcggctgg ccaatgcgat ccgggcgtac cgcggcggct acatcgccga
     1081 gcagcggcgc gagattgagc ggcagctctt ctcgggggag ctgatgggcg tcgtcagcac
     1141 ggtggcgctc gaactcggga tcgacatcgg cagtctcgac gcctgcctga ccgtgggata
     1201 cccggggtcc atcgccagcc tgtggcagca agccggccgg gcggggcgtg gtaccgagga
     1261 ctcattggcg gtcctcattg gctacaactc acccatcgat cagttcctga tgcagaaccc
     1321 gggctatctg ttctcgcaga gccccgagat ggccgtggtc gacccggcga atccgcacat
     1381 cgtggcctgg cacctgcgtt gcgcctgcca cgaactcccc gtgtcggcgg ctgatgaggc
     1441 catctttggc gagtacctgt acagcgtgct ggacatcctc accgacagcg gcgacatccg
     1501 ccggcggggg aaccgctggt actgggcggg acagggctac cccgcgacgc agttctcgct
     1561 gcggaacatc cccgaagacg tataccagat catcgaccag actgacggcc ggtccgagac
     1621 catcggtgag accgatgaga cgtcggcatt cttctcgctg tacacgcagg ccatctacat
     1681 gcacaacgcc gagacgtact tcgtcaagga gctcaacatc gaccggaaac atgcgtacat
     1741 cgagaaggtg gactgtgact actacaccca gtcggtcgac aagcgcgaga tccgggtggt
     1801 caacgccgac gaggagcgga agtggcgcgt cagccaggtg tttttcggtg acgtgaccgt
     1861 caccgagatg gttgtcatgt tccggaagat ccagttcggg gcccgtgaca gcatcgggtt
     1921 tgggagcgtc gacctcccgc ccatcgagac cgacaccgag gcgctgtggg tcgtgccgcc
     1981 gcgcgacgca ctggaccgta tccggcagtt cggccgggtg ccgcaggacg gcctgctggg
     2041 ggtcgggaac gtcatcgtgg gcgtcatgcc cctcttcgtt atgtccgatc ccatggacgt
     2101 gggcgcggcc gtggactcga gtaacgtggg atccccgagc ctgttcatct atgacaagca
     2161 tgcgggtggc gtgggcttcg cgcagcgggc gtatggccgc attgaggaga tcatggaagc
     2221 ggcgctgtcg ctcatccacg ggtgtgactg ttccgacggc tgtccatcgt gtgtgggggc
     2281 tccactgccg ccctatgcct acggtgacgt ggacagttcg agccgcggag cgatccccga
     2341 caaggaggcg gccctgtgtc tgctgcattc gctgctggag cgcgagcctt ataccccgag
     2401 gcccgtgtcg tcgccactgg gcgaggcggc tgcgccactg ccgaggggcc ctgagccaac
     2461 gtctctcacg gcgccggcac caccgccacc cccggactac gcgcgcct
//
LOCUS       DAOSPL010000649         2502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_41674_length_2502_cov_3.456069, whole genome shotgun sequence.
ACCESSION   DAOSPL010000649 DAOSPL010000000
VERSION     DAOSPL010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41674_length_2502_cov_3.456069"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="PLQ54_11905"
     CDS             <1..997
                     /locus_tag="PLQ54_11905"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOS94011.1"
                     /translation="PSNKLELFMWLDSDRMSNAVLREFYVERDVVADERRMRVDANPY
                     GYFREEMLSVYFTAHPYGRPVGGWADDIQRITRQQAADFFATYYAPNNATLVLVGDLE
                     PEATFAMAERYFGRVPRSPKPIADLITQEPTQHGPRRIEVSTMARPVVTINYHVPPRQ
                     HPDFYPLSIAADVLNGRSGRLHKRLVLADDIAFSVAANMDGSAHAGTFTLIAMPKGPN
                     THQRVEDTLVAEIDRLKSEPPTGDEMQRVKNAADAEFIGGLRSNDGLAMQLAVMDAII
                     DWREVIEAPAKIKAVTADQVAEVAKKYLIRDNSTVGWVTTKPMPAGQASPGGGAQ"
     gene            994..2478
                     /locus_tag="PLQ54_11910"
     CDS             994..2478
                     /locus_tag="PLQ54_11910"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOS94012.1"
                     /translation="MTARQRSPQLTALVTISLCLLTSLVAAQPIVDHPSQLVFPELRF
                     DPPSAEQFRVELRDGLVVYIQEDHTLPTFQLSLSWIGGSYFDPPGKEGLAGLTYALLR
                     SGGTTRMTADQVDERLEFLSAQIGTSAGLYEGTASCWCLSRDTDEVLGLLADILLRPA
                     FEEDKIRREREEALETLSHRYDSPASLLGARLPKLLYGDAPPGRTVTGASVMGITRDD
                     LIAFHKNFVGANKAILTVSGDFAREEMLDKLSDALAEFVPTGATMPDRDERLELPGAA
                     TPATYLLNKAGPQAQGQMCHLGIERHDPDFVPLRVLNYVLGGGGLSDRLARRVRGDEG
                     LTYAISSTVEAPYGRRGAVGVSFSCGANNAAYVVSLVREEIDRIRAEEVTDEELTIAK
                     DAFLGTFPDAFSTPYDVIANLAALERAEVPLDFYSRVRDEIAAVTRADLLRVAREHWR
                     PDALLIVLAGDAAAMQAGNPEKPATFGDAARVHVIEPKDPVACE"
BASE COUNT          432 a          850 c          811 g          409 t
ORIGIN      
        1 gccctcgaac aagctcgagc tgttcatgtg gttggattcg gaccgaatgt cgaatgcggt
       61 actgcgcgag ttctacgtcg agcgggacgt ggtggccgat gagcggcgga tgcgcgtcga
      121 cgccaacccg tatgggtact tccgcgagga gatgctgtcg gtctacttca ccgcacaccc
      181 gtatggccgc ccggtgggcg gatgggccga cgacatccag cggatcacgc gtcagcaggc
      241 cgccgacttc ttcgccacct actatgcccc gaacaacgcc accctggtgc ttgtgggcga
      301 cctggagcct gaggcgacct tcgccatggc ggaacgctat ttcggtcggg tcccgcggag
      361 cccgaagccg atcgccgacc tgatcaccca ggagcccacg cagcacggcc ctcggcggat
      421 cgaggtctcg accatggcac gcccagttgt gaccattaac taccatgtgc cgccccgcca
      481 acacccggac ttctacccgc tgagcatcgc cgccgacgtg ctcaatggcc ggtccgggcg
      541 actgcacaag cgcctggtgc tcgccgacga cattgcgttc agcgtggccg cgaacatgga
      601 tgggtcggcc catgccggga ctttcacact catcgcgatg cccaaggggc cgaacacgca
      661 ccagagggtg gaggacacac tggtggccga gatcgaccgc ctcaagtccg agccgccgac
      721 cggcgacgag atgcagcgag tgaagaacgc ggccgacgcg gagttcatcg gcggtctccg
      781 gtccaacgat ggcctcgcca tgcaactcgc cgtgatggac gccatcatcg actggcgtga
      841 ggtcatcgag gccccggcga agatcaaagc ggtcacagcc gaccaggttg ccgaagttgc
      901 caagaagtac ttgatccggg acaacagcac cgttgggtgg gtgaccacaa aacccatgcc
      961 cgcgggtcaa gcctcgccag gaggtggcgc gcaatgacgg cccgccagcg atccccccaa
     1021 ctcactgctc tagtcaccat ctcgctgtgc cttctcacct cactggtcgc cgcccagccc
     1081 atcgtcgacc atcccagcca gctggtgttt cccgaactgc ggttcgaccc accgtcggcc
     1141 gaacagtttc gggtcgagct cagggacggg ttggtggtct acatccagga ggaccatacg
     1201 ctcccgacct tccagctctc gctctcgtgg atcggcggaa gctacttcga cccgccgggc
     1261 aaggaggggc tggccggcct gacctatgcc ctactccgca gcggcggtac cacccggatg
     1321 acggccgacc aggtcgacga gaggctggag ttcctctcgg ctcagatcgg gaccagtgcc
     1381 ggcctgtacg agggcaccgc ctcgtgctgg tgcctgtcgc gtgacacgga cgaggtgctg
     1441 ggcttgcttg ccgacatcct cctgcgaccg gccttcgagg aggacaagat ccgtcgcgag
     1501 cgcgaggagg cgctggagac cctcagccac cggtacgaca gcccggcctc gctgctgggc
     1561 gcgcgtctcc cgaagttgct ctacggcgat gccccgcccg gcaggacggt cacaggcgcg
     1621 tcggttatgg gcatcacgcg cgatgacctt atcgcgttcc acaagaactt cgtgggtgcc
     1681 aacaaggcca tcctgaccgt atcaggcgac tttgcccggg aagagatgct ggacaaactg
     1741 tcggacgcgt tggccgagtt cgtgcccacc ggtgccacca tgccggatcg cgacgagcgg
     1801 ctcgagctgc ccggcgccgc cacgccggcg acgtacctgc tcaataaggc cggcccccag
     1861 gcccagggcc agatgtgcca cctgggcatc gagcggcacg atcctgactt cgtcccgctg
     1921 cgagtcctca actacgtgct cggcggtggc gggctctccg atcgactcgc acgacgggtg
     1981 cgcggggatg aaggtctcac atacgccatc agttcgacgg tggaggcccc gtacgggcgc
     2041 cggggcgccg tgggcgtctc cttcagctgt ggggccaaca acgccgccta tgtcgtgtcc
     2101 ctggtgcgag aggagatcga ccgcatccgt gcggaggagg tcacggatga ggaactgacc
     2161 atcgccaaag atgcattcct cgggacattc cccgacgctt tctcgacgcc ctatgacgtg
     2221 atcgcgaacc tggccgcgct cgagcgcgcc gaggtccctc tcgacttcta cagccgggtc
     2281 cgcgacgaga tcgcggcggt gactcgggcc gacctgctcc gtgtcgcgcg ggaacattgg
     2341 cggcccgatg ccctcctcat cgtgctggcc ggcgatgcgg cggccatgca ggccggcaac
     2401 ccggaaaagc ccgccacgtt cggggacgcc gcgcgggttc atgtcatcga gccgaaggac
     2461 ccggttgcgt gtgagtaggt gagctccctc gacggttgcg gg
//
LOCUS       DAOSPL010000650         2501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_41704_length_2501_cov_3.060098, whole genome shotgun sequence.
ACCESSION   DAOSPL010000650 DAOSPL010000000
VERSION     DAOSPL010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41704_length_2501_cov_3.060098"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="PLQ54_11915"
     CDS             <1..616
                     /locus_tag="PLQ54_11915"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS94013.1"
                     /translation="RLVLLEQRDPAATVDDHTKAQQARAMVLGNLGLAHIAAGADAQS
                     AVHGSVDPRNAKGNLETAVAVLEQAVMANSGSAAAWNDLGMAYMRNRQYFSAEAAFQQ
                     AVTLQGSSDLYRWNLEACRECIDPSGSGGGGTTAAAGMPAGGGMMGPGMMGSGPGMMG
                     PGPAGGSGPGMMGPRGGMMGPAGGGSAPGSGGGGSTASAPGGAM"
     gene            725..>2501
                     /locus_tag="PLQ54_11920"
     CDS             725..>2501
                     /locus_tag="PLQ54_11920"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOS94014.1"
                     /translation="MKSTGSGRTYWVAIAVLLATSMLPAWSLESGALFVTSKPSGQDL
                     FVNGKLVGKTPLTLENLTPGKYRVEVRSDQYLDASQDSIEVKAQELASVAFELKPARG
                     SLLLRSDLVGAAITLDGEAAGTVSADTPFERQDLAIGRHVVTLSGPWLRVTREFGVTK
                     GEPAIVDIAAKQHAGTIEITSDLPIQWGQLSGRPLEGTAPLPLTNIQPGEHEIILGLT
                     PVPYRDMVTVRAGETTTVSLRGGEHLGTILISADLTAENVSVRVDDVLVSDSTPSRVQ
                     GVIAGSHIVEVRAVRQPDKVKVVGQRVVFVPPGGTALAAFKTTDLLTDRPKCPDGMVW
                     VPQRSNAPGEYAFSQPHDEGTPNTVVRDPAWRPDGIDPYGRRAAVIVDAGGTVLKLDK
                     AASVVATHLSRSSEAKRVNQGSPLLFGTGSGTSAGGPGMMGGGMTPEGGPASGGMQGA
                     PAGVMQGPGAGSSAPPAAMRGSGPGMMGGGPGMMGPGMMGGGAAGGGGPGFITAYDAE
                     GKRKSNPLDDTFTDYVLQWDAVSGMNFCIDKYEYPNFPGATPVAATLEQARSLCAAKG
                     KRLCSATEWVAACSGPASWLFPYGNQ"
BASE COUNT          411 a          833 c          899 g          358 t
ORIGIN      
        1 ccggttggtg ctcctcgaac agcgcgaccc ggcagccacc gtcgatgacc acaccaaagc
       61 ccagcaggcc cgcgccatgg tgctcgggaa cctggggctg gcccacatcg ccgccggggc
      121 ggatgcccag tcggcggtcc acggtagcgt ggatcctcgg aatgccaagg ggaacctcga
      181 aacggccgtc gcggtcctcg aacaggccgt catggcgaac tcgggctcgg cggccgcctg
      241 gaacgacctg ggcatggcct acatgcgcaa tcggcagtac ttctcggccg aggccgcctt
      301 tcagcaggcg gtgaccctgc agggcagcag cgacctctac cgctggaacc tggaagcctg
      361 ccgtgagtgc atcgacccca gcgggtcggg cggtggcggc accaccgctg ccgcgggcat
      421 gccggccggc ggcgggatga tggggcctgg catgatgggc agcggcccgg gcatgatggg
      481 ccccggcccc gcaggcggaa gtggcccggg gatgatggga ccgcgcggcg gaatgatggg
      541 accggctggc ggcggctctg ccccaggtag tggcggcggc gggtcgacgg cctcggcgcc
      601 gggcggagcc atgtaggcgc gagtgttcgg cgggacctgg ccacaggcca gagccacacc
      661 gggtaatccc gccggggtgg acggtcacca ccggcgggcg cgttgagctg gaggaagtga
      721 cgtcatgaag tcgacaggca gcggccggac ctactgggtc gccatcgcgg tgctcctcgc
      781 gacgtctatg ctccccgcat ggagtctcga gtcgggggcg ctcttcgtca cctccaaacc
      841 cagcggacag gacttgttcg tcaacggcaa actcgtgggc aagactccgc tgacgctcga
      901 gaacctgacg cccggcaagt accgcgtcga agtgcgcagc gatcagtacc tcgatgcctc
      961 gcaggacagc atcgaggtga aggcccagga gctggcctcg gtggctttcg agctcaagcc
     1021 agcccggggc agcctcctgc ttcggtccga tctggtcggg gccgccatca cgctggacgg
     1081 tgaggccgcg ggcaccgtgt cggccgacac ccccttcgaa cgtcaggacc tggccatcgg
     1141 ccgccatgtg gtcacgctga gcggcccgtg gctccgggtc accagagagt tcggcgtcac
     1201 caagggcgag ccagccatcg tcgacatcgc cgccaagcag catgccggga ccatcgagat
     1261 caccagcgac ctgcccatcc agtgggggca gctgagcggc cggcccctag agggaacggc
     1321 tccccttccc ctcaccaaca tacagccggg cgagcacgag atcatactgg gcctgactcc
     1381 cgtgccctac cgtgacatgg tgaccgttcg ggcgggcgag accacaacgg tctcgctgcg
     1441 tggcggcgag cacctgggca ccatcctgat cagcgcggac ctcaccgctg agaacgtcag
     1501 tgttcgcgtg gacgatgtgc tggtcagcga ctcgactccc agccgcgtcc agggggtcat
     1561 cgcaggctcg cacatcgttg aggtcagggc cgtgcgccag cccgacaagg tcaaagtcgt
     1621 cgggcagcgc gtcgtgtttg tgcctccggg cgggactgct ctggccgcat tcaagacaac
     1681 ggacctgctc acggatcggc ccaagtgccc cgacggcatg gtgtgggtgc ctcagaggag
     1741 caatgcgccc ggcgagtacg cattttctca gccgcacgat gagggaaccc cgaacaccgt
     1801 cgtccgcgat cccgcttggc gccccgacgg gatcgacccg tacggccggc gtgccgccgt
     1861 catcgtcgac gcgggtggga ctgtgctgaa actggacaag gcggcgtcgg tcgtggccac
     1921 ccacctgagc cggtcgagcg aggcgaagcg cgtcaaccag gggagccctc tgctctttgg
     1981 cacggggtcg ggcacatcgg cgggcgggcc gggcatgatg ggcggcggga tgacgccgga
     2041 aggcggcccc gccagcggcg gaatgcaggg cgctcccgcc ggcgtgatgc agggaccggg
     2101 agcggggtcc tccgctccgc cggcagcgat gaggggcagt ggccccggta tgatgggcgg
     2161 cggtccgggt atgatggggc cgggtatgat gggcggcggc gctgccggcg gcggcggacc
     2221 cggtttcatc acggcctatg acgcggaggg gaaacgaaag tccaacccac tcgatgacac
     2281 gttcactgac tacgtcctcc agtgggacgc ggtcagtggg atgaacttct gcatcgacaa
     2341 gtacgagtac cccaacttcc ccggcgccac gccggtggcg gccacgctag agcaggcccg
     2401 aagtctgtgc gcagccaagg gcaagcgcct gtgctccgcg accgagtggg ttgccgcgtg
     2461 ctcgggaccc gcctcgtggc tgttcccata cggcaaccag t
//
LOCUS       DAOSPL010000651         2499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_41739_length_2499_cov_3.420213, whole genome shotgun sequence.
ACCESSION   DAOSPL010000651 DAOSPL010000000
VERSION     DAOSPL010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41739_length_2499_cov_3.420213"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1493)
                     /locus_tag="PLQ54_11925"
     CDS             complement(36..1493)
                     /locus_tag="PLQ54_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HOS94015.1"
                     /translation="MRVRASLAAWCCVTALVTTRGLCGVATFVDRTAELGLAIDTNAA
                     CWVDIDNDGWVDLCAGGAVWRNDDGKRFARLADVGTVVSADYDNDGYADLFSWSSLRL
                     YHNDGGKGFAEAPLPQLPRCVSRGACWTDFDNDGSVDLYIGGYEDWDAGVTYPSIALR
                     NTGDGLFVVAWTDERYRARGITACDFDEDGDADIYASNYRLQPNVLWLNDGTGHFTDE
                     AAGYNAVATSEGFDGGHSIGAAWGDFDNDGHFDLFAGNFAHVDDRGDQPKSRFLRNLG
                     PDASHSFEDMGPCGVFYQESYATPAAGDYDNDGDLDLFFTTVYGTASFGRPNHPVLYR
                     NDRGFAFADATAAAGLGGLPPTSQAAWADFDNDGDLDLVGGGKLFVNQGTGGHWLKVR
                     LEGDGRAVNRWAVGAQARVSIDGGIVTRQVEAGTGEGNQNDLTLHFGLGAWAEPVTLE
                     VRWPGGMVQRVENVLPDSTRIVRHGEANRGTGLGT"
     gene            1875..>2499
                     /locus_tag="PLQ54_11930"
     CDS             1875..>2499
                     /locus_tag="PLQ54_11930"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HOS94016.1"
                     /translation="MGILPLVLSTLVGGSDVGPAYGRVQTVLGPVEPWQLGPTLTHEH
                     VLVDFVGADRVSPERYDADEVFRTMLPYLASLKDAGIETLVECTPDYLGRDVGLLKRL
                     SLASGVRLVTNTGLYKDPYLPAWAHVASAEELALHWIEEARSGIGPERIRPGFIKIAA
                     NEGELVDVQRRIVRAAALASKATGLPIAAHTTQGTSALQELDILADVG"
BASE COUNT          376 a          870 c          826 g          427 t
ORIGIN      
        1 agcagcggat tagccacgac ttcctcggcg acatgtcagg tacctagccc ggtcccccgg
       61 ttggcctcgc cgtgccgcac gatgcgtgtg gagtccggaa ggacgttctc gacccgctgc
      121 accatgccac ccggccaccg gacctccagt gtcaccggct cggcccatgc gcccagcccg
      181 aagtggaggg tcaggtcgtt ctggttcccc tctcccgttc cggcctcgac ctgacgagtg
      241 acgataccgc cgtcgatgga cacgcgcgcc tgcgccccga ccgcccatcg gttcaccgcg
      301 cgcccatcgc cctcaagccg gaccttcagc cagtgtccgc cggtgccctg gttcacgaag
      361 agctttccgc cgcccaccag gtcgaggtct ccgtcgttgt cgaagtcggc ccacgccgcc
      421 tggctcgtcg gcgggaggcc tcctaacccc gctgccgccg tggcgtcggc gaacgcgaag
      481 ccccggtcgt tccggtacag cacggggtga ttgggccgcc cgaacgatgc cgtgccgtag
      541 accgtggtga agaacaggtc gaggtcgccg tcgttgtcgt agtcccctgc cgccggcgtg
      601 gcatacgact cctggtagaa cacgccgcac gggcccatgt cttcgaacga atggctggca
      661 tcggggccca ggttccgcag gaagcgcgac ttgggctggt cgccccggtc gtcgacatgg
      721 gcgaagttgc ccgcgaacag gtcgaagtgc ccgtcgttgt cgaagtcgcc ccaggcggcg
      781 ccgatcgagt ggccgccgtc gaacccctcc gaggtcgcca ctgcgttgta gcccgctgct
      841 tcgtcggtga agtgccccgt gccgtcgttt agccatagca cgttcggctg cagccggtag
      901 ttcgatgcgt agatgtccgc gtcgccgtcc tcgtcgaagt cgcaagcggt gattccgcgg
      961 gcgcggtagc gctcatcagt ccaggccacc acgaagaggc cgtcgcctgt gttccgcagg
     1021 gcgatgctgg gataggtgac cccggcgtcc cagtcctcgt acccgccgat gtacaggtcg
     1081 acggacccat cgttgtcgaa gtcggtccag caggcgcctc tcgatacgca ccgaggcagt
     1141 tgggggagcg gcgcttcggc aaagcccttg ccgccgtcgt tgtggtacag acgcaacgat
     1201 gaccacgaga acaggtcggc atagccgtcg ttgtcgtagt cggccgaaac caccgtgccc
     1261 acgtccgcca gccgtgcgaa tcgcttgccg tcgtcgttcc gccagacggc tccgccggca
     1321 cacaggtcca cccaaccgtc gttgtcgata tcgacccagc aggcggcgtt cgtgtcgatc
     1381 gccagcccga gctcggccgt acggtcgacg aaagtcgcca cgccgcacag accgcgggtg
     1441 gtcaccagag ccgtcacaca acaccacgct gccaagctcg ctcgcacgcg catccagctc
     1501 ttgccctccg cgattccgca cccgctcttg gctccgggcg cggtggcacc gccatgcact
     1561 ctcggctccg cgtccgagcg tttcgacact cgagggatcg cgccctcgcg ccattggtct
     1621 gatagcatag gggtcgactc aaccccgtgt gtccgtcgac ttcggcgagc aggcgccggc
     1681 tgccgtgggc catcaacgat gagcgggtca cggcggtagc ggctcatgga ctgcccgccg
     1741 gcccggcttg gccgttgtgg ggcccgggga acccggatcc tccgagcgga gccgaaggga
     1801 taccgtggcc tctcggcgga cttggggtag gtgacccctg ggattggacg gatggcgagc
     1861 ggagctggtc aaccatgggc atactcccac tcgtgttgtc gacgctggtc ggtggttccg
     1921 atgtgggccc ggcatacggt cgggtccaga ccgtgctggg ccccgtcgag ccgtggcaac
     1981 tcgggcccac gctcacgcac gagcatgtgc tggtcgactt cgtgggtgcc gatcgggtct
     2041 cgcccgagcg ctacgacgcc gatgaggtgt tccgcaccat gctgccatac ctcgcatcgc
     2101 tcaaggacgc cgggatcgag acgctggtcg agtgcactcc cgactacctg gggcgtgacg
     2161 ttggtctgct gaagcgtctc agccttgcca gcggcgtgag gctggtgacc aacaccgggc
     2221 tctacaagga cccttatctg ccggcctggg cgcacgtggc ctcggcggag gagctggccc
     2281 ttcactggat cgaggaggcc cgctccggca tcggcccgga gaggatccgg cccgggttca
     2341 tcaagatcgc cgcgaacgag ggcgaactgg tcgatgtgca gcgcaggatc gttcgcgcgg
     2401 cggcgctggc gtcgaaggcc accggtctcc ccatcgcggc acacacgact cagggcacct
     2461 cggcgctcca ggagttggat atcctggccg acgtcggct
//
LOCUS       DAOSPL010000652         2497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_41796_length_2497_cov_2.379607, whole genome shotgun sequence.
ACCESSION   DAOSPL010000652 DAOSPL010000000
VERSION     DAOSPL010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41796_length_2497_cov_2.379607"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="PLQ54_11935"
     CDS             <1..1056
                     /locus_tag="PLQ54_11935"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS94017.1"
                     /translation="KTRQRLFEDHVNYQQGLLWFLTHDPRVPETIRRELRHWGLARDE
                     FTDTGGWPRQLYVREARRMVSDYVMTENNCMGNAVAPDSVGLASYTMDSHNCQRFAKD
                     GRALNEGDVQIPVPAPFPISYRSIIPRRGEPGNLLVPVCLSASHIAFGSIRMEPVFMV
                     LAQSAATAAKLAIDGGTAVQDVPMDLLTARLREDGQVLVWEPPTVAPLIDPQSLPGIV
                     LDDTSASRTGEWLPSNMASRRRVGTGYIHDGNAGKGAKSLTYEPEVPTAGRYRLTLMS
                     VPHENRATHVPVTIEVPGQAPVTVYVDQRAAGDGGSAVLGVFDLPAGHGLRVTVSNAE
                     TDGYVVADGLQLLLEDD"
     gene            1130..2482
                     /locus_tag="PLQ54_11940"
     CDS             1130..2482
                     /locus_tag="PLQ54_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOS94018.1"
                     /translation="MSPSALAFTASALAAAGLVAAWPPERPSGTTPTPEQLAWQDLEY
                     GMFVHFSINTFNDMEWSDGTLSPSTFNPTAFDPKQWARVAKDAGMKYLVFTAKHHDGF
                     CTWPSAYTDYSVRSSPWRDGKGDCVREVADACREAGLRFGIYLSPWDRHAPSYADNAA
                     YDRYYTDQIRELLTNYGDVMEWWCDGAGGEGHVYDWAGYYRALKQLQPSCLQAIAGNS
                     DIRWVGNEDGLAPDTLWNVVHVDGQPYWWPAECDVPLRRNWFWHTDDADSAKSLDHLL
                     DIYHRSVGHGAGLLLNVAPDRRGLIPEADAARVRELWDTVCRLYRLDMARSRACDASS
                     STPLHPARLACDGDPNTYWRAAEGDARPWITVKLGDTRRIDRVVLEEYLPLGQHVSGF
                     RVQCFDGDEWTNVFEGTTIGHKRIAVFAAVPAQVVRVRLQTEAGTPALRSLGVYLGEE
                     "
BASE COUNT          416 a          866 c          820 g          395 t
ORIGIN      
        1 aagacgcgcc agcggttgtt cgaggaccat gtgaactacc aacaagggct gctgtggttc
       61 ctgacccacg acccgcgcgt gcccgagacc atacgccgag agctccgcca ctgggggctt
      121 gcgcgcgacg agttcaccga taccggcggg tggccgcggc aactctacgt ccgcgaggcc
      181 cggcgaatgg tctcagacta tgtgatgacc gaaaacaact gcatgggcaa cgccgtagcc
      241 ccagactcgg tgggtttggc ttcctacacc atggattcgc acaactgcca gcggttcgcg
      301 aaagatggcc gggctctgaa tgaaggcgat gtacagatcc ccgtgcccgc gccattcccc
      361 atcagctacc gatcgatcat tccccgtcga ggtgagcccg gcaatctgct ggttcccgta
      421 tgcctctcgg cctcgcacat cgcctttggg tcgatccgga tggaacccgt cttcatggtg
      481 ctcgcgcagt ccgcggccac ggcggcgaag ctggccatag acgggggaac tgcggtccag
      541 gatgtgccca tggacttgct caccgctcgg ctgcgcgagg acgggcaggt cctcgtctgg
      601 gagccgccca cggtggcgcc gctcatcgat ccgcagagcc tacccgggat cgtgctcgac
      661 gacacatcgg ccagccgaac tggggagtgg ctgccctcga acatggccag ccgccgccgg
      721 gtcggcaccg ggtacatcca tgacggcaac gcgggcaaag gcgccaagtc gctgacgtac
      781 gagcccgaag tgcccactgc cggccgctac cggctcacac tcatgtccgt cccccacgag
      841 aaccgcgcca cccacgtgcc ggtcaccatc gaggtgcccg gccaggcgcc cgtcacagtc
      901 tacgtggacc agcgcgccgc cggcgacggg ggcagcgccg tcctgggcgt gttcgatctg
      961 cctgccggcc acgggctccg agtcacggta tcaaatgccg agaccgatgg ttacgtggtg
     1021 gccgacggcc tgcagctgct cctcgaggac gactgagccg cgtgccgctg ggccaagggc
     1081 gtcgcccagc ggcgccggct gcgcacgagg ccatggaaga ggtggtcaga tgtcccccag
     1141 cgctctcgcg ttcaccgcgt cggctctcgc ggcggccggg cttgtcgccg cgtggccgcc
     1201 cgaacggccg tcaggcacga cgccgacgcc cgagcaactc gcatggcagg acctggaata
     1261 cggcatgttc gtgcacttct cgatcaacac gttcaatgac atggagtgga gcgacggcac
     1321 gctcagcccc tccacgttca accccacggc cttcgatccg aagcagtggg cgcgtgtcgc
     1381 caaggacgcg ggaatgaagt atctggtgtt caccgccaag catcacgacg gcttctgtac
     1441 ctggccgtcg gcctacaccg actacagtgt ccgcagcagc ccttggcgtg atggcaaggg
     1501 cgattgcgtg cgcgaggtgg ccgacgcctg ccgcgaggcg gggctgcggt tcggcatcta
     1561 cctctcgccc tgggatcgcc acgcgccgtc ctacgccgac aacgccgcct acgaccgcta
     1621 ctacacggac cagatacgcg agctgctcac gaactatggc gacgtcatgg agtggtggtg
     1681 cgacggcgcg ggcggcgaag ggcatgtgta tgactgggcc gggtactacc gcgccctgaa
     1741 gcagctgcag ccatcgtgtc ttcaggcgat cgcgggcaac tccgacatcc gctgggtcgg
     1801 caacgaggat ggcctcgcgc ccgacacgct gtggaacgtg gtccacgtgg atggccagcc
     1861 ctactggtgg ccggccgagt gcgatgtccc ccttcggcgc aactggttct ggcacacgga
     1921 cgacgcggac tccgccaagt cactcgacca cctgctcgat atctaccacc gctccgtggg
     1981 ccacggcgcc ggcctgctcc tcaacgtggc ccccgaccgg cgcgggctga tccccgaagc
     2041 cgacgcagcg cgcgtcaggg agctctggga cacggtctgc cggctatacc ggctcgacat
     2101 ggctcgcagc cgggcgtgcg atgccagctc cagcaccccc ctccatccgg cacgtttggc
     2161 ctgcgacggc gaccccaaca cctactggcg cgccgccgag ggtgacgcgc ggccctggat
     2221 cacggtgaaa ctgggcgaca cgcggcgcat cgaccgcgtc gtgctcgagg agtacctgcc
     2281 cctcgggcaa cacgtcagtg ggttccgcgt gcagtgtttc gacggagacg agtggacgaa
     2341 cgtctttgag ggcacgacca tcggtcataa gcgcatcgcc gtcttcgccg ccgtgccggc
     2401 acaggtggtg cgcgtccggt tgcagaccga ggcgggcaca ccggccctgc gttcccttgg
     2461 cgtgtacttg ggcgaggagt gagtggccac ggcattc
//
LOCUS       DAOSPL010000653         2491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_41947_length_2491_cov_2.472496, whole genome shotgun sequence.
ACCESSION   DAOSPL010000653 DAOSPL010000000
VERSION     DAOSPL010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41947_length_2491_cov_2.472496"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2418)
                     /locus_tag="PLQ54_11945"
     CDS             complement(<1..2418)
                     /locus_tag="PLQ54_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94019.1"
                     /translation="MTESIAPRRKSLLLVVSVLALLVLLLPCLGQAPEGLTLLDDPGT
                     GTYDLCFRVGSVSDLRGEIALSQTGASEGLVVALDGTQAQVLSMRDGRRTALSPAAPL
                     PRREGPVGVAVRRRLGRVDLVVDGALVTRGYTDAPAEGALGYRLPDGVVQELLVQPID
                     EHIEFGEDFAWEADSQARDLWQVVSGEWTDQKAGDWTNAAAEARQTAAQGANLFAYVG
                     RADGPALVVAGMEFWSDYSVSVAARGASNDPMGLVAYYSDPDDYLLLRITSQLATTDD
                     ADQVQLILCRDGDRTVLGSAAGGFVPEQWYRLTLRMCDGRVEALVDGQRVLAIDGFRD
                     LARGKIGLYCEGQAGVKFDDVEVGEWRRFGDDFSGGASPAWVGSREGWATEGSGDGRK
                     HVLCRTAEPRSVLVTSGGGWARYAFSADVDLSAGSGGLCAGFRDTRNHLVLEWDGLEG
                     GGLPATGERATTWLRLVRVLDGETTRLGEVPVGVPAGGWTTLSLVADADLLRAFVDGK
                     QAFEVINGELQGGQVGLMGWLGARFDDVRVEVPEENKAAVVTAQFTREDTMREWASVA
                     GSWIAPSDPEQQPAIWWNKGDYYGDVTVELTVPGVGTPEQVERVLLLSDGVSAESGYV
                     FELSGAPGGGLGIGLLRQGVPVAQTVVPDVAFPAQVKLAKRGWLLSASVEDEPKLVHL
                     DRDPLTGSKIGLWPAGLDIPPSDVLATSSAQRDYPFSSAPVDWITRMGIWDVTSRWTC
                     SPQWTWFSGVADDGPAAMWTKREFQGDVTVELYASIKMGLASGGASYKHPNDLNITLL
                     GDGANLST"
BASE COUNT          454 a          936 c          737 g          364 t
ORIGIN      
        1 cgtcgacagg ttggccccat cgcccagcag cgtgatgttc aagtcatttg ggtgcttgta
       61 cgaggcgccg cccgaggcca agcccatctt gatggacgcg tacagctcca cggtgacatc
      121 gccctggaac tcgcgcttcg tccacatggc cgccggcccg tcatcggcaa cacccgagaa
      181 ccacgtccac tgcggcgagc acgtccagcg gctggtgaca tcccaaatcc ccatccgagt
      241 gatccagtcc acaggggccg acgagaatgg atagtcccgc tgggccgacg aagtcgccag
      301 cacgtcactg ggcgggatgt ccagtccagc cggccatagc ccgatcttcg acccggtcag
      361 tggatcgcgg tccagatgca ccagcttggg ctcatcctcg accgacgcgc tcaacagcca
      421 tccccgcttg gccagcttca cctgggccgg gaaggccaca tccggaacca ctgtctgggc
      481 cacgggcact ccctggcgca ataggccgat ccccaggccg ccgcctgggg cgccggagag
      541 ttcgaacacg tacccgctct ccgccgacac gccatcgctg aggagtagca cgcgctcgac
      601 ttgctcgggc gtgcccacgc ccgggacagt gagctctacg gtgacatcgc catagtagtc
      661 gcccttgttc caccagatgg ccggttgctg ctccggatca ctgggcgcga tccaggagcc
      721 ggccacgctg gcccattcgc gcatcgtgtc ctcgcgggtg aactgcgcgg tgacgacggc
      781 ggccttgttc tcttcgggga cctcgacacg aacgtcatcg aagcgggcac cgagccaccc
      841 catcaggccc acctggcccc cctgaagctc accgttgatc acctcaaacg cctgcttgcc
      901 atccacaaag gcgcggagca ggtccgcgtc ggccaccagc gatagcgtgg tccaaccccc
      961 ggcagggaca ccgacgggca cctcgcccag ccgcgtggtc tcaccatcca ggactcgaac
     1021 cagccggagc catgtcgtgg cgcgttcccc cgtggccgga agccctccac cctccagacc
     1081 gtcccactcg agaaccaggt ggttgcgcgt gtcacgaaag ccggcacaca ggcccccgga
     1141 cccggcactc agatccacgt cggccgagaa cgcgtatcga gcccaacccc caccggaagt
     1201 aaccagcacc gaccgaggct cggccgtccg gcagagcaca tgctttcggc catcgccgga
     1261 tccctcggtg gcccaccctt cgcggctgcc gacccatgcc ggcgacgccc ctccggagaa
     1321 atcgtcgcca aaccgccgcc actcaccgac ctcgacgtca tcgaacttca ccccggcctg
     1381 gccctcgcag tagagcccga tcttgccccg ggccagatcg cgaaaaccat cgatcgccag
     1441 tacgcgctga ccatccacca gggcttcgac acggccgtcg cacatgcgga gcgtcagacg
     1501 gtaccactgc tccgggacga acccaccggc tgccgagccg agcacggtcc ggtctccgtc
     1561 tcggcacagg atcagctgca cttggtccgc gtcatcggtt gtcgctagct gggaggtgat
     1621 ccgcagaagt agataatcgt cgggatccga gtagtaagcg acaagaccca tcgggtcgtt
     1681 actcgcgccg cgagccgcca cactgaccga gtagtcgctc cagaactcca tcccggcaac
     1741 caccagggcg ggcccgtcgg cacgccctac gtacgcgaac aggttcgccc cctgagccgc
     1801 cgtctgacgg gcctccgccg cggcgtttgt ccagtccccc gccttctggt ccgtccactc
     1861 gccgctcacc acctgccaca agtccctggc ctggctgtcg gcctcccagg cgaagtcctc
     1921 accgaactcg atgtgctcat cgatcggttg aaccagcagc tcctgcacta cgccatccgg
     1981 gagccggtag cccagggccc cctcggccgg agcgtcggta tagccccgag tgaccagcgc
     2041 gccgtccacg acgaggtcca cacggcccag gcgtcgtcgc actgccaccc ccactggacc
     2101 ctcgcgccgg ggaagtggcg cggccggcga cagagcggtt cgtcggccgt cgcgcatcga
     2161 gagaacctgg gcctgggtcc cgtcgagggc gaccacgagg ccctcggacg cgccggtctg
     2221 actgagggcg atctcacccc gcaggtcgga cacgctgccg acgcggaagc acaggtcgta
     2281 cgtgccagtc ccgggatcgt ccaggagagt gagaccctcg ggagcttggc ccagacacgg
     2341 aagtaggagc acgaggagcg ctagcacact gaccactagc aacagcgact ttcgccgcgg
     2401 cgcaatactc tcagtcatag cccgccggac agcctccaat ggggagaacg cgccagaaga
     2461 tcagcggact tccgtacaat ccgtacccga c
//
LOCUS       DAOSPL010000654         2487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42031_length_2487_cov_3.240954, whole genome shotgun sequence.
ACCESSION   DAOSPL010000654 DAOSPL010000000
VERSION     DAOSPL010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42031_length_2487_cov_3.240954"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="PLQ54_11950"
     CDS             complement(<1..1122)
                     /locus_tag="PLQ54_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94020.1"
                     /translation="MHPSPGVHRVMGIVGVCAAGVVLLWLAPGAPGQTPGEREGDTTM
                     AARTTNSNPIDIGSRLELFVDRYLIDTMDPRLSLELHPPTPREIVMVHDAPWEGSGTG
                     YFDVFRDGDTIRMYYIGYGLTNEDGTVMSTRPVCACYMESTDGIHWTKPDLGLIDFAG
                     TTHNNVVWIRESADNFVVFKDTNPNCRPGEEYKATASGPGGLWAYKSSDGLRWSPLQD
                     APIITKGAFDSQNLAFWDPLRGHYWCYFRDFHDGLRDIRVATSADFLTWSEPEMLAYE
                     DSPDEQLYTNQIMPYPRAPHIFVGFPTRYVERAWSDSFLPLPDLQHRQNRMKIAPRYG
                     TAITDGLFMSSRDGRTFRRWGEAFIRPGPERKDNWLYGDG"
     gene            1437..2183
                     /locus_tag="PLQ54_11955"
     CDS             1437..2183
                     /locus_tag="PLQ54_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94021.1"
                     /translation="MWHGEFSVTRWLGLVCLIVVTGWVSGCGGGAPTSDNPAASSTGI
                     RTGGGSIPDGEDVSGVVVSSTNTLPPLVRSAGADAYVPAEGAQVVLEGPDGAREVCVT
                     GSDGRFAFRAPHSGRSLLRFDLNGNSNAADIQVKVRGGKRTEVVAGLVPEDSQAPAGS
                     RDRIRIAADAPWVRPGEPVYVRAFLQRAATGCPEIVWLMETRTDASIFPTSDPNVLEV
                     RAGWGTGWVEVQARLERAESNTVRLFVGPR"
     gene            2268..>2487
                     /locus_tag="PLQ54_11960"
     CDS             2268..>2487
                     /locus_tag="PLQ54_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94022.1"
                     /translation="MARDSAGAPEGSGARMSPELEAEARERLSDLCLLVQAQAGPADP
                     RGLIQSCPRLVALAESLGDRKAQAHAWHH"
BASE COUNT          373 a          783 c          909 g          422 t
ORIGIN      
        1 gccgtcgcca tagagccagt tgtccttccg ttcgggcccc ggccggatga acgcctcgcc
       61 ccagcggcgg aaggtgcggc cgtcgcggct ggacatgaac agcccatcgg tgatggcggt
      121 gccgtagcgg ggcgcgatct tcatccggtt ctgccggtgc tgcaggtcgg gcaacgggag
      181 gaacgagtcc gaccacgcgc gctccacata ccgcgtcggg aagcccacga agatgtgcgg
      241 cgcccgcgga tagggcatga tctggttggt gtagagctgc tcgtcgggcg agtcctcata
      301 ggccagcatc tcgggctcgg accacgtgag gaaatcggcc gacgtggcca cccgaatgtc
      361 gcgcaggccg tcgtggaagt cgcggaagta gcaccaatag tgcccgcgca gggggtccca
      421 gaacgccagg ttctggctat cgaaggcgcc cttggtgatg atgggcgcat cctgcagcgg
      481 cgaccagcgc aggccgtcgg acgacttgta ggcccacagg ccgccggggc ccgatgccgt
      541 ggctttgtac tcctcgccgg gccggcagtt ggggttcgtg tccttgaaga caacgaagtt
      601 gtcggcgctc tcgcggatcc acaccacatt gttgtgggtg gttccggcga agtcgatgag
      661 cccgagatcg ggcttcgtcc agtggatgcc gtcggtgctc tccatgtagc aggcgcacac
      721 ggggcgagtg gacatgacgg tgccgtcctc gttcgtcagc ccatacccga tgtagtacat
      781 gcggatggtg tcgccatccc ggaacacgtc gaagtagccc gtgccgctgc cttcccaggg
      841 cgcatcgtga accatcacga tctcgcgcgg cgtgggcgga tgaagttcca gcgacagcct
      901 tgggtccatc gtgtcgatca gatagcggtc cacgaacagc tcgagccgcg agccaatgtc
      961 gatggggttc gagttcgtcg tccgcgccgc catggtcgtg tctccttctc tctcgccggg
     1021 cgtctgcccc ggagcaccgg gagccagcca aagcaagacg actcccgccg cgcatacgcc
     1081 gacgatgccc atgacgcggt ggacgcctgg tgatgggtgc aagcgagcgc gcctcccata
     1141 ggattccgtc gtcatgagcg gcactcttcg gtaggcgtcg ttcgccgtcc tcccccgcca
     1201 actgcctgcg gcctgcgcgg ggcgggcacg cgccatgcgg acacccacgt gactgtcacg
     1261 gcgaccgctt gtcagtgtgg aaggatggca tcggagagcc agcgcccgcc gtcgccgaag
     1321 cccggcgagc ggctgcgttg gcgatggggg tggcagtcgg ggtcggcgcc ggagcggcaa
     1381 ggtctggcgg gggtccccgg cagggcgctc ggcgtgggaa gtggggggcg aggctgatgt
     1441 ggcatggcga gttcagcgtg acgcggtggt tgggtctggt gtgtctcatc gtggttaccg
     1501 gttgggtgtc ggggtgtgga gggggcgctc cgacgtcgga caatcccgct gcctcgtcga
     1561 cgggcatacg cacgggcggc ggcagcatcc ccgatggcga ggacgtcagc ggggtggtgg
     1621 tgtcctccac caacacgctg ccccctctgg ttcgttcggc cggtgccgac gcgtatgttc
     1681 ccgccgaggg ggcccaagtg gtcctcgagg ggcccgacgg agcgcgcgag gtgtgcgtga
     1741 ccgggtccga tggccggttc gccttccgtg ctcctcacag cggccggtcc ctcttgcgat
     1801 tcgatctcaa cggaaactcg aatgcagccg atatccaagt gaaggtcagg ggtggcaagc
     1861 ggaccgaagt ggttgcgggt ctggtgcccg aagacagcca ggcccccgcc ggcagcaggg
     1921 atcggatccg gattgcggcg gacgcgccat gggtgcgtcc cggcgagccg gtctatgtcc
     1981 gggcgttcct ccagcgcgcc gcgacgggtt gtccggagat cgtgtggctg atggagacgc
     2041 gaactgacgc gtccatcttc cccacttcgg acccgaacgt gctggaggtg agggccgggt
     2101 ggggcacggg ttgggttgag gtccaggcgc gcctggagag ggctgagtcg aacacggtgc
     2161 ggctgttcgt tggaccgcgc tagcgacgcg tcgcaccgga tcggctggca ccgtcgagtg
     2221 ggccttacgg ccccagcaac ctggctgcca accaagggag agcgaccatg gctcgtgatt
     2281 cagccggcgc gccagagggc tcgggcgccc gcatgtcgcc cgagctcgaa gctgaagcgc
     2341 gcgaacgcct ttccgacttg tgcctcctgg ttcaggctca agcgggtccg gccgacccgc
     2401 gagggctgat ccagtcctgc ccgcgcctgg tggctctggc cgagtccctg ggagaccgca
     2461 aggcccaggc gcatgcctgg caccatc
//
LOCUS       DAOSPL010000655         2487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42038_length_2487_cov_2.366365, whole genome shotgun sequence.
ACCESSION   DAOSPL010000655 DAOSPL010000000
VERSION     DAOSPL010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42038_length_2487_cov_2.366365"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLQ54_11965"
     CDS             complement(<1..189)
                     /locus_tag="PLQ54_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003297106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HOS94023.1"
                     /translation="MYLSRLILDPRSRQVASELERPYEMHRTLTRAFDDGGQGDRVLF
                     RADVHPRTGVPTVLVQSEA"
     gene            complement(171..905)
                     /gene="cas5e"
                     /locus_tag="PLQ54_11970"
     CDS             complement(171..905)
                     /gene="cas5e"
                     /locus_tag="PLQ54_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HOS94024.1"
                     /translation="MAVLLLRLAGPMQSWGTQSRFVDRDTGLEPSKSGVLGLVCAALG
                     IDREDDAALARLAECRLGVRVDREGAMSRDYHTAGGGSVPGRRTYGVAKASGGTPDAV
                     VSTRHYLADASFLVGLEHADRAMLERIDACLDGPFWPIYLGRKSFVPGEPVRVIGGLR
                     TGTLEDVLRAEPWRPRTAREREGVLRDGLRMVIESVAPTPEVRMDQPLSFANGRRRCA
                     VRYVSTSVVAGESIVIEEEQPCTSLA"
     gene            complement(908..2023)
                     /gene="cas7e"
                     /locus_tag="PLQ54_11975"
     CDS             complement(908..2023)
                     /gene="cas7e"
                     /locus_tag="PLQ54_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HOS94025.1"
                     /translation="MFVELHLIQSFGPHCLNRDDTNQPKDCTFGGFRRARISSQCIKR
                     SIRWHEAFRDATAPHAALRTRWIHAKLADLLRQRGRDATVDSPVIKAYVAALGLKFDK
                     NDRTQYGLYVGLDEVDRMAADLDAKWDTLAGASDAQMKKEVEPIAKALKLRTQAADIA
                     LFGRMIADRPGDNVDAACQVAHAISTHKVDMEMDFFTAVDDLKQADDEPGAGMMGTVD
                     FNSSCFYRYALLDFSKLVANLGGDVALARASAEGFLRAAVAAIPTGKQSSFAAHNPPE
                     FILAVVREKGAWSLANAFEKPVRATAETGLTEASVEALDGYFSRLASLYGTDGIVTRP
                     ACWLSDAKAEHAPDPKVGSLEELIGTVMDALPKAEEG"
     gene            complement(2037..>2487)
                     /gene="casB"
                     /locus_tag="PLQ54_11980"
     CDS             complement(2037..>2487)
                     /gene="casB"
                     /locus_tag="PLQ54_11980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HOS94026.1"
                     /translation="LADLRRGAGKPPGTEPRTFRYVVPWASGQGAQAERRLYLVASLF
                     ALHSSGAAEGNLGTTFRQIAARGESESTEKRFVALLNCNVEDLPNHLRHAVSLAKSRE
                     VPINWRQLLADLRHFDHEDRYVQRQWAGAFWQHQSSATAETESDTHS"
BASE COUNT          386 a          882 c          820 g          399 t
ORIGIN      
        1 cgcctcggac tgcaccagca cggtgggaac gccggttcgc ggatgcacat cggcgcgaaa
       61 cagcacccgg tcgccctgcc cgccgtcatc gaacgcccgc gtcagcgtcc ggtgcatctc
      121 atagggccgt tccagctcac tcgccacctg ccgcgagcgg gggtcgagga tcaggcgaga
      181 gaggtacatg gctgctcctc ctcgatgacg atggactccc cggcgacgac gctcgtgctg
      241 acataccgga ccgcgcaccg gcggcggcca ttggcgaagc tcagaggctg gtccatgcgc
      301 acctccggcg tcggtgccac ggattcgatg accatccgca gcccatcgcg gaggacaccc
      361 tcgcgctctc gagccgtccg agggcgccac ggctccgcgc ggaggacgtc ctcgagagtg
      421 ccggtccgga ggccgccaat cacacgaaca ggctccccag gcacgaagct cttgcggccg
      481 aggtagatcg gccagaaggg gccgtccagg caagcgtcga tccgctccag cattgcgcgg
      541 tcggcgtgct cgaggcccac caggaagctc gcatcggcca ggtagtggcg cgtcgagaca
      601 acggcgtccg gcgtcccgcc gctggccttg gcgaccccat aggtccgccg ccccgggacg
      661 ctccccccgc cggctgtatg gtagtcccgg gacatggctc cctcgcggtc gacgcggacg
      721 cccaggcggc actcggccag ccgagcgaga gcggcatcgt cctcgcggtc gatccccagg
      781 gccgcgcaca ccaagcccag cacgccactc ttcgagggct ccagtcccgt gtcgcggtcc
      841 acgaagcgac tctgcgtgcc ccacgactgc atgggtcccg cgagccgcag gagcaggacg
      901 gccatgccta cccctcctcc gccttcggca gcgcgtccat caccgtgccg atgagctcct
      961 ccagactgcc gaccttgggg tcgggcgcgt gctccgcctt ggcgtcggag agccagcacg
     1021 cgggccgcgt gacgatgccg tcggttccat acagggacgc caaccggctg aagtagccat
     1081 cgagcgcctc gaccgaagcc tcggtcagcc ccgtctcggc cgtcgctctc acgggtttct
     1141 cgaaggcgtt cgccagcgac cacgcgccct tctctctcac caccgcgagg atgaactccg
     1201 gcggattgtg ggccgcgaag ctgctctgct tgccggtggg gatggcggcc accgccgcgc
     1261 gcaggaaccc ctccgccgac gcccgtgcca gcgcgacgtc gccccccagg ttggccacga
     1321 gcttgctgaa gtccaggagc gcatagcggt agaagcaaga gctgttgaag tccacagtgc
     1381 ccatcatgcc ggccccgggc tcgtcatcgg cctgcttcag gtcatcgacc gcggtgaaga
     1441 agtccatctc catgtcgacc ttgtgcgtcg agatggcgtg ggcaacctga caggccgcgt
     1501 cgacgttgtc ccccgggcgg tccgcgatca tgcggccgaa cagcgcgatg tcagccgctt
     1561 gggtccgcag cttcagggcc ttcgcgatgg gctcgacttc cttcttcatc tgcgcgtcgg
     1621 acgcgcccgc gagggtgtcc cacttcgcat ccaggtcggc ggccatccgg tcgacctcgt
     1681 cgagtccgac gtacaggccg tattgcgtcc gatcgttctt gtcgaacttc aggcccagag
     1741 cagccacata tgccttgatg accgggctgt ccaccgtggc gtctcggccc cgctggcgca
     1801 gcaggtcggc cagcttggcg tgaatccacc gggtgcgaag ggccgcgtga ggcgccgtag
     1861 cgtcgcggaa cgcttcatgc caccggatgc ttctcttgat gcactggctc gagatcctcg
     1921 cgcggcggaa gccgccaaac gtgcagtcct tcggctggtt ggtgtcatcg cggttcaagc
     1981 agtgtggacc gaagctctga atcaggtgca gttcaacaaa cacggggcat ccctcctcag
     2041 gagtgggtgt cgctctcggt ctcggccgtg gcggaactct ggtgttgcca gaacgcaccc
     2101 gcccactggc gctggacata gcggtcctcg tggtcgaagt ggcgcaggtc ggccaggagc
     2161 tggcgccagt tgataggtac ctccctggac ttcgcgaggg agacagcatg gcgcaggtga
     2221 ttcggcaggt cctcgacgtt gcagttgagc agcgcgacga agcgcttctc ggtgctctcg
     2281 ctctcgccgc gcgccgcgat ctgacggaag gtcgtaccca gattgccttc ggcagcaccc
     2341 gaggagtgca gcgcgaagag cgaagcaacg aggtagagcc tgcgctcggc ctgcgccccc
     2401 tgtccgctcg cccagggcac gacataccgg aacgtgcgtg gctccgtgcc gggcggcttc
     2461 ccggcgcccc ggcgcagatc ggccagc
//
LOCUS       DAOSPL010000656         2485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42070_length_2485_cov_3.614403, whole genome shotgun sequence.
ACCESSION   DAOSPL010000656 DAOSPL010000000
VERSION     DAOSPL010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42070_length_2485_cov_3.614403"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="PLQ54_11985"
     CDS             <1..1108
                     /locus_tag="PLQ54_11985"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS94027.1"
                     /translation="DGAMGIGKLYQGPLPPFEERVISVEGNVRTWVDEWGVTRADAVN
                     QPTDGFATRKYLDFPVKTPADFEEMKGHFDPHTPERTLPVDTVTPSLNPDGYRHHTPG
                     GVHWRDLVDACNQADVPVMASVSGLYWTARDWCGFEGLSYMVYDQPLLVHEMMEYWTW
                     FIMELLREPLSHIQVDVMVINEDMAFKPQAMLSPASIREFMRPRYERLYEFLKSHGVG
                     TVLMDSDGYNGQILEVLYPTGLDGICPVEIAAGNDPEEMLTRFPGVVLQGGIDKRELR
                     FGRDQLRAEVAKRYAAARRHGGYVPHVDHGVPPDIPLRNFLYYVELARGYCDGEDLEN
                     YEPPCELEKQLGPIEEMFDPRSAIAAAFAMDKAE"
     gene            1131..>2485
                     /locus_tag="PLQ54_11990"
     CDS             1131..>2485
                     /locus_tag="PLQ54_11990"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOS94028.1"
                     /translation="MHVHCAAGLLGTMMAAAALPAEDLTVLPHGEGAAAPPVMLTQYL
                     KARASQALRARLDGYEAIKSAADVLAYQDRMKAFMARQLGGLPERGPVVSRVVGRIGR
                     EGFHIEKVLFESQPGIWVSGLLYVPAGQGPFPAVLVACGHSDNGKAYEQYQRAPSLLA
                     RNGIAAFCYDPIGQGERGQILDEQGRQKYPSTLEHTLVGVGAMLIGRNTAYYRVYDGM
                     RAIDYLQSRADIDGTRIGCTGTSGGGTLTSYLMALDDRIVAAAPSCYLTSLERLFVTE
                     GPQDAEQNIAAQLAFGMDHADYIIMRAPRPTLICCATRDFFDILGTWDSFRQAKRAYT
                     RLGAAEAVDLIESDDEHGFNAPHRVAMVRFMRRHLLGVDGPVEDIDMPMLTDEELRCT
                     PEGQVLRMDGARSVLALNADHEAALAANRQGLWESSPLPEMLRAVRNASCRARLANHL
                     S"
BASE COUNT          433 a          831 c          825 g          396 t
ORIGIN      
        1 cgacggcgcg atgggcatcg ggaagctcta ccaaggccca ctgccgccgt ttgaggagcg
       61 cgtcatctcg gtggagggca atgtgcgcac ttgggtcgac gagtggggcg tgacgcgcgc
      121 cgatgctgtg aaccagccca ccgacggctt cgcgacccga aagtacctcg acttccccgt
      181 caagacgcca gcggatttcg aggagatgaa gggccacttc gacccgcaca cgcccgagcg
      241 tacccttccc gtcgacacgg tcaccccaag cctcaacccc gatggctacc gccaccacac
      301 ccccggcggg gtccactggc gcgaccttgt cgacgcgtgc aaccaagccg atgttcccgt
      361 gatggcctcc gtctccgggc tctactggac ggcacgcgac tggtgcgggt ttgaggggct
      421 ctcctacatg gtctacgacc aacccctcct cgtgcacgag atgatggagt attggacctg
      481 gttcatcatg gagctgctgc gggagccgct ttcgcacatt caggtcgacg tgatggtcat
      541 caacgaggac atggccttca agccacaggc catgctctcg cctgcatcga tacgcgagtt
      601 catgcggccg cgttacgaac ggctgtacga gttcctcaag tcgcacggcg tcgggacggt
      661 tctgatggac tccgacggct acaacggcca gatcctcgag gtgttgtacc ccaccgggct
      721 cgatggaatc tgccccgtcg agatcgcggc cggcaacgat cccgaggaga tgctgacgcg
      781 ctttcccggc gtggtgcttc agggcgggat cgacaagcgc gagctgcgct ttggccgcga
      841 ccagctccgg gccgaggtgg cgaagcgcta cgccgcggcc cggaggcatg gcggctatgt
      901 gccccatgtc gaccacggtg tgccgccgga catcccgctg aggaacttcc tatactatgt
      961 cgagctcgca cgcggctact gcgatggtga ggatctcgag aactacgagc cgccctgcga
     1021 gctcgagaag cagcttggcc ccatcgagga gatgttcgac ccgcggtcgg ccatcgccgc
     1081 cgcgttcgcc atggacaagg ccgagtagcg cccggaagga ggcgtctggg atgcacgtgc
     1141 actgcgcagc aggactgctc gggaccatga tggccgcggc ggcgttgccc gccgaggatc
     1201 tgacggtcct tccgcatggg gagggggccg cggcaccgcc ggtcatgctg acgcagtacc
     1261 tcaaggcgcg cgcgagccag gccctgcgcg cgcggctgga cggctacgag gccatcaagt
     1321 ccgccgccga cgtgctcgcc taccaggacc gcatgaaggc gttcatggcc cggcagctcg
     1381 gtggactccc ggaacgcggc ccggtggtgt cccgcgtggt cgggcgcatc gggcgcgagg
     1441 ggttccacat cgagaaggtg ctgttcgaga gccagccagg catctgggtc tccgggctct
     1501 tgtacgttcc ggccgggcag gggccgttcc ccgccgtgct ggtcgcctgc ggacacagcg
     1561 acaacggcaa ggcctatgag cagtaccagc gggcgccatc actgctggct cggaacggca
     1621 tcgcagcctt ctgctacgac cccatcggcc agggcgagcg gggccaaatc ctcgacgagc
     1681 agggccggca gaagtacccc tcgaccctcg agcacacgct ggtgggcgtg ggcgccatgc
     1741 tgatcggccg caacacggct tactaccgcg tctacgatgg catgagggcc atcgactacc
     1801 ttcagagccg agcggacatc gatggcacgc gcatcggctg cacaggcacg tcgggcggcg
     1861 gaacgctgac gagctacctg atggcgctgg atgaccgtat cgtggcggcg gcgccgagct
     1921 gttacctgac ctcactcgaa cgactgttcg ttaccgaggg gccacaggac gcggaacaga
     1981 acatcgcggc gcagctcgcc ttcggcatgg accacgccga ctacatcatc atgcgcgcgc
     2041 cgaggcccac gctgatctgc tgtgccacga gagacttctt cgatatcctc ggcacgtggg
     2101 acagcttccg ccaggccaag cgtgcctaca cgcggctggg tgccgccgaa gccgtagacc
     2161 tgatcgaaag cgacgacgag catggcttca acgcgccgca ccgagtagcc atggtccgct
     2221 tcatgcggcg ccatctgcta ggcgtagacg gcccagttga ggacatcgac atgccgatgc
     2281 tgaccgatga ggagctgcgc tgcacgcccg aggggcaggt cctccggatg gacggggctc
     2341 ggtcggtact cgccctcaac gccgaccacg aggcagcgct ggccgccaac aggcaggggc
     2401 tttgggagtc ctcacccctc ccggagatgc tccgcgcggt gcgcaacgcc tcctgccgag
     2461 cccgattggc gaaccacttg tcgag
//
LOCUS       DAOSPL010000657         2485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42073_length_2485_cov_3.020165, whole genome shotgun sequence.
ACCESSION   DAOSPL010000657 DAOSPL010000000
VERSION     DAOSPL010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42073_length_2485_cov_3.020165"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="PLQ54_11995"
     CDS             <1..1183
                     /locus_tag="PLQ54_11995"
                     /inference="COORDINATES: protein motif:HMM:NF013978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bis-aminopropyl spermidine synthase family
                     protein"
                     /protein_id="HOS94029.1"
                     /translation="AAILVYPCRHTVRRMGDGSVAGDVLQEVAEAARLREGAEGVRAL
                     LRVVHAQGPIRLRDAAIQVRLPLPVAAAVRREMEKRGLLTRDAGMRLSESGRRLAEGD
                     LGIAVTADQTCTTCGGRTIAVGAEHASLLEAMRDALAGRPSADVKLDQSHGTPETALR
                     RALLLSETGLLAGRDILFLGDDDLTSIAAALARRHLGLGDRGRLAVAEVDPRLCEFIR
                     VTAKRLGVGVEVHAADLRQPLPAHLVARFDGFVTDPPYTVPGAALFVSRGVAALRSGP
                     LRTGLLSFGHKDPGTMARVHLHLAQMGLAVVRVLPRFNEYEGAAILGNTSQMIELATS
                     DATRPLVAGAYEGSLYTADQERKMAGPRQPSTGGRSRAGATSSGSGGAKRGSRRKKPK
                     P"
     gene            1180..1512
                     /gene="speD"
                     /locus_tag="PLQ54_12000"
     CDS             1180..1512
                     /gene="speD"
                     /locus_tag="PLQ54_12000"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOS94030.1"
                     /translation="MTRRHLVADLWVRDVEPLRFEEPLRTALRSGIDKSGATILGELF
                     HQFEPHGISGCFLLAESHVSYHSFVEEKLLSLDIYTCGSADVERILDASRRVLNPVRE
                     QVRDVPRG"
     gene            1496..2428
                     /locus_tag="PLQ54_12005"
     CDS             1496..2428
                     /locus_tag="PLQ54_12005"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOS94031.1"
                     /translation="MYLGVEGRAAEFGLSPLDRDGAQRFARAAARHGKSLWYYYTPFL
                     LGLERSTQRRVFTGEVDGSLVMLVYRNRRRRETCDLVVPPLPVSDAALAVARRAAHAL
                     APQARHRIMWCDEADITWARSVADGDPVDQAPDLVYDPDRVAALESSAYRNVRKRVAR
                     VRREHAVAVRPAGREDLPTCRELLQAWFEARSEAIRPIGDMGYTRGALTLLGELEPPL
                     FEAWLYEVDGRTAALSVGGDIRPGCVCFFCLKTLPSVPRLSTYVRWDSLSRWRGRGLV
                     NDGSHLDRPGLAQHKRGFRPVAELPVWQVWLRTD"
BASE COUNT          351 a          811 c          910 g          413 t
ORIGIN      
        1 ggcggccatt ctggtgtacc cttgccgcca caccgtgcga cggatgggag acggttcggt
       61 ggcgggcgac gtgctgcaag aggtggcaga ggcggctcgg ctgcgcgagg gtgccgaggg
      121 ggtgcgggcg ctgctccgcg tggtccacgc gcaagggccc atccggctgc gtgatgccgc
      181 catccaggtg cgtctgccgc tccccgtggc ggcggcggtc cggcgagaga tggagaagcg
      241 agggctgctc actcgcgacg ccgggatgcg gctctccgag tccgggagac ggttggccga
      301 gggcgatctc ggcatagcgg tcacggcaga ccagacctgc accacgtgtg gaggccggac
      361 catcgctgtg ggggcagagc acgcgtcgct gctcgaggcg atgcgagacg cgttggctgg
      421 ccggccgtcg gccgacgtga agctggacca gtcgcatggt acgcctgaga ccgcgctgcg
      481 gcgagcgctc ctgctgtcgg agacggggct gcttgcgggg cgcgacatcc tcttcctggg
      541 tgacgacgac ctgacatcca tcgcggccgc gttggcccga aggcacctgg gactgggcga
      601 tcgagggcgt ctagccgttg cggaggtcga tcctcggctg tgcgagttca ttcgcgtcac
      661 ggccaaacgg ctgggcgtgg gggtcgaagt ccacgccgca gacctccggc aaccgctgcc
      721 ggcccacctg gtggcccgtt tcgacgggtt cgtgaccgac ccgccctata cggtgcctgg
      781 ggcggcgctg ttcgtctcgc gcggcgtggc ggccctgcgg tctggtccct tgcgcacggg
      841 gctgctgtcg ttcggccaca aggatcctgg cacgatggct cgggttcacc tacacttggc
      901 gcagatgggc ctggccgtgg tacgggtgct gcctcgcttc aacgagtatg agggggccgc
      961 gatccttggc aacacgagcc agatgatcga gctggcgacg agcgacgcga cgcggccctt
     1021 ggtcgccgga gcctatgagg gcagcctcta cacggccgat caggagcgga agatggccgg
     1081 acctcggcag ccgtcgacag gaggccggtc ccgggccggc gcaacctcat cgggctccgg
     1141 cggcgcgaag cgcggatcga gacggaagaa gccgaagcca tgacccggcg tcatctggtg
     1201 gcggatttgt gggtacggga cgtcgaacca ctccggttcg aggaaccgct gcgcacggcg
     1261 ctgcgttctg gaattgataa gtcaggcgcc acgattcttg gggagctatt ccaccagttc
     1321 gagccccacg gcatctccgg ctgtttcctc ctggccgagt cgcacgtgag ctaccattcg
     1381 ttcgtcgagg agaagctgct gtctctggac atatacacgt gcggcagcgc cgacgtggag
     1441 cgaatcctcg atgcgagccg tcgtgttctc aaccccgtgc gggagcaggt ccgcgatgta
     1501 cctcggggtt gagggccgcg ccgccgagtt cggcctgtca ccgctcgatc gcgatggggc
     1561 gcagcgtttc gcccgggcgg cggcacggca cggcaagagt ctatggtatt actacactcc
     1621 attcctcctc ggcttggagc gatcgaccca acgccgcgtg ttcactggcg aggtggacgg
     1681 atcgctggtg atgcttgttt accgcaaccg gcggcggcgg gagacctgcg atctcgtggt
     1741 gcctccattg ccggtgtcgg acgcggctct cgccgttgcg cgccgggcgg cgcacgcact
     1801 ggccccgcaa gcccggcacc gcatcatgtg gtgcgacgag gccgacatca catgggcgcg
     1861 gtcggtggcc gacggcgatc ccgtagacca ggctcccgac ctggtctacg atcccgaccg
     1921 ggtagcggcg ctggagtcca gcgcctaccg taatgtgcgc aagcgcgtgg cgcgggtccg
     1981 tcgcgagcac gccgtcgcgg tccggcccgc ggggcgcgag gatctgccca catgccggga
     2041 gctactccaa gcctggttcg aggcacggtc cgaggccatc aggcccattg gggatatggg
     2101 ctacacccgc ggcgcactga cgctgctggg cgagctcgag ccgcccctgt tcgaagcctg
     2161 gctctacgag gtcgacggcc gtaccgccgc gctgagcgtc ggaggtgaca tccgccccgg
     2221 gtgtgtgtgc ttcttctgcc tgaagacgct gccgagtgtg ccgcggctgt cgacgtatgt
     2281 ccgctgggac agtctgagcc gctggcgggg ccgcggcctc gtcaacgacg gcagccacct
     2341 cgaccgtccc ggcctggccc agcacaagcg cggcttccgg ccggtggccg aactgcccgt
     2401 gtggcaggtc tggctgcgaa cggactgatc ccccggggcc ggtgcctact cccggatctt
     2461 cgtcgcctgg gccaggtcca ggtcg
//
LOCUS       DAOSPL010000658         2485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42074_length_2485_cov_3.016461, whole genome shotgun sequence.
ACCESSION   DAOSPL010000658 DAOSPL010000000
VERSION     DAOSPL010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42074_length_2485_cov_3.016461"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2258)
                     /locus_tag="PLQ54_12010"
     CDS             complement(<1..2258)
                     /locus_tag="PLQ54_12010"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOS94032.1"
                     /translation="MHLLAALTLAPTLMTAAAPDARGPQVVFRADLNGSVAPQVGRGE
                     ASTQGTPEFAPGRHGRAFVADGQCVLSYSADGNLDKARGTIGMWVCPQWDGADGQNHS
                     FFADDLDFNQPAANNLHLWKWLSGDSLRFDIRTEELRDIEFGIADWKAGEWHHLAVAW
                     DCQAGTRLFIDGRQVAVLELEWEPKPGTRFYIGANWSGGSPAQALIEDVVIYSAPLDA
                     GQVARVATGQALPAVRPTQLKAPDTITIGRPFAVTLACEAAEATPDELPLSVTLDGLP
                     LPCVEGQATARLRPGAATYGPLHFELPAYYHLVPGEHELAASLAGAVVADAAPWATTV
                     QVSRPPAGDARRAWRFSHRKVLRGEDVYLAPGEGVAFWFDGAVRPYDRAGQELCESLV
                     ESGRIVDVLPCRLVDEVDCSRTDHGFREWGTSHVSALGDGRPYRITGTRESVREQREV
                     YGNTASVVPGFQYRLASAPRPVPHLLVVDTLNDRERYLETAIDVAPGSGVAPLLAATG
                     VGATDLTNLNVTYTGREYALDGQPFQQITLFYPKSDAVTVTITDSRRELQQDEGTGAA
                     VARLSVYEVTADLADLPAEVDEAERSVSLFYPWVSPLYSEYGFCASTEGTRRASVDTL
                     TDYLRFMGFSRIEFHPYQFGRSAEFDSDIFPRQGDADVLEDTLPITKAKGIEVVPRID
                     STCFYLSDDAGKTLYADREAYQLTRRGETMRFFGLVPDPLHPQVQQLLQDMLCELARK
                     TKGWPNVPAVGFR"
BASE COUNT          388 a          862 c          839 g          396 t
ORIGIN      
        1 cggaagccga cggccggcac gttgggccag cctttggtct ttcgcgccag ctcgcagagc
       61 atgtcctgca ggagttgctg cacctgcggg tgtagaggat cgggaacgag gccaaagaag
      121 cgcatcgtct cccctcggcg agtgagctgg taggcctctc tgtccgcgta cagtgtcttg
      181 cccgcatcat cggacaggta gaagcacgtg gagtcgatgc gcggaacgac ctcgatcccc
      241 ttcgccttgg tgatcgggag ggtgtcctcg aggacgtcgg catcgccttg gcgcgggaag
      301 atgtccgagt cgaactccgc gctccggccg aactggtaag ggtggaactc gatgcggctg
      361 aagcccatga accggaggta gtcggtgagt gtgtccacgg aggcacgccg cgtgccctcg
      421 gtgctcgcgc agaagccgta ctcgctgtac agcggcgaaa cccacgggta gaacaggctg
      481 acgctgcgct cggcctcgtc cacctcggcc ggcaagtcag cgaggtcggc cgtaacctcg
      541 tagacagaca ggcgagccac ggccgcgccc gtgccttcgt cctgctggag ctcgcggcgc
      601 gagtcggtga tcgtgaccgt caccgcatcg ctcttcgggt agaagagcgt gatctgctgg
      661 aatggctgcc cgtcgagcgc atactcgcgc ccggtgtacg tgacgttcag gttggtgagg
      721 tcggtcgcgc ccacgccggt ggccgccagg agtggggcga cgccgctgcc cggcgccacg
      781 tcgatcgcag tctcgaggta gcgctcgcgg tcgttgaggg tgtcaacgac caacaggtgc
      841 ggaaccggcc gcggcgcgct cgccagccgg tactggaacc ccggcacaac gctcgccgtg
      901 ttgccgtaga cctccctctg ctccctgacg ctctcacgag tgcccgtgat gcggtagggc
      961 cgtccgtcgc ccagcgcgga cacatgcgag gtgccccact cgcgaaagcc gtggtcggtc
     1021 cggctgcagt ccacctcgtc gaccaaccgg cacggcagta catccacgat acggcccgac
     1081 tccaccaacg actcgcatag ctcctgcccg gctctgtcgt aggggcgcac ggcgccatca
     1141 aaccagaagg cgacgccctc gcccggcgcc aagtacacgt cctccccgcg cagaaccttg
     1201 cggtgcgaga accgccaggc ccttctggca tcgcccgcgg gcggtcgcga gacctggacc
     1261 gtcgttgccc acggcgccgc gtcggccaca accgccccgg ccaggctcgc ggcaagctca
     1321 tgctcgccag gcacgaggtg gtagtacgcg gggagttcga agtgcagtgg accataggtg
     1381 gccgcgcctg gccggagcct agccgtcgct tggccctcga cgcacggcaa gggcaggccg
     1441 tccagcgtca ccgacaaggg cagttcgtcc ggcgtagcct cggcggcctc gcaggcgagc
     1501 gtcaccgcga acggccggcc gatggtgatc gtgtccggtg ccttcagttg tgtcggcctg
     1561 accgccggca gggcttggcc cgttgccact cgtgccacct gcccggcgtc aagcggcgcg
     1621 ctgtagatca ccacgtcctc gatgagggcc tgggccggcg agccgccgct ccagttggcg
     1681 ccgatgtaga accgcgtgcc cggcttgggc tcccactcga gttccaggac cgcgacctgc
     1741 cggccatcga tgaacagccg cgtcccggcc tggcagtccc acgcgaccgc gaggtggtgc
     1801 cattcgccgg ccttccagtc ggcgatgccg aactcgatgt ctctcagctc ctccgtgcgg
     1861 atgtcaaatc ggagcgagtc gcccgacagc cacttccaca ggtgcaggtt gttcgcggcc
     1921 ggctggttga agtcgaggtc atcagcgaag aagctgtggt tctgcccgtc ggccccgtcc
     1981 cattgcgggc agacccacat gccgatggtc cctcgcgcct tgtcgaggtt gccgtccgcc
     2041 gagtagctca ggacgcactg gccgtcggcc acgaacgcac gcccgtgcct ccccggggcg
     2101 aactcgggcg tgccctgcgt ggaggcttcg cccctgccga cctgcggagc cacgctgcca
     2161 ttcagatcgg cgcggaagac aacctgaggc cccctggcgt cgggtgcagc cgctgtcatg
     2221 agcgtcgggg ccaaggtgag tgcagcgagc aggtgcatgg gcggacctcc agtgtgtgta
     2281 tctggttcgg catcaagagt caccggcccg ggtggcgatg acttggggca ccgtctgtgc
     2341 cggcactccc caaccgtctg tcccaagcgc acccggtcca tggcggagca cccgcccaag
     2401 gcacgacgga caccaccgga ctgcgcgacc gcgccgaatg gctttccgcg ggcgcgaacc
     2461 gtgcgtacct accgccggaa agact
//
LOCUS       DAOSPL010000659         2481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42150_length_2481_cov_3.279885, whole genome shotgun sequence.
ACCESSION   DAOSPL010000659 DAOSPL010000000
VERSION     DAOSPL010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42150_length_2481_cov_3.279885"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLQ54_12015"
     CDS             complement(<1..323)
                     /locus_tag="PLQ54_12015"
                     /inference="COORDINATES: protein motif:HMM:NF021645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HOS94033.1"
                     /translation="MHRYAITVLLIASSAAHADLFDYIALPDDSFAYEFTGTETALLG
                     KIHSIHMTSQTWQGIKWEHEIRIYEPPRVEYPDLVGMLITGGNPSKGEDMLGLTVAGT
                     AGGRAS"
     gene            756..947
                     /locus_tag="PLQ54_12020"
     CDS             756..947
                     /locus_tag="PLQ54_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94034.1"
                     /translation="MSKQRYTVANLKPKEGPTTRRGDDTVRGGRTDWYGRRAGYDSLE
                     ALIAGDCRGPGTIRGRIVA"
     gene            1111..1701
                     /locus_tag="PLQ54_12025"
     CDS             1111..1701
                     /locus_tag="PLQ54_12025"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOS94035.1"
                     /translation="MSGLPDVVQGYLRRRGWERRAREAAVHVIWEQVVGEQIADHTET
                     IFCRDGKLVVTASDPSWAHAISALRHQLATKLNEALGEPTIRDIHLQSKGLGVATRGS
                     APIRRRDLEFAGGMKVTDEELEATPISPEVRSRIESTVRGLRHPELRDMVYAALLRES
                     RISAWKRAHGYRECPRCAALHNDAHELCGDCRILPR"
     gene            1939..>2481
                     /locus_tag="PLQ54_12030"
     CDS             1939..>2481
                     /locus_tag="PLQ54_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94036.1"
                     /translation="MNRLKPLFAFSVLAAVVGLARSAEAQAPPTVRQRVTIAQIVSLT
                     DGLPYKATAIALEDTLALKLRHTGRCEVTDRQQFLRAARAQNLPVNDPAEIRAALVEL
                     GQHYGILGTVTAEGTLVNAQISIVVVKPAERTTAVLTNLEIKGGTVGMLGFMSAVAER
                     VATEMQIPMDDATRAVMNSAS"
BASE COUNT          412 a          833 c          831 g          405 t
ORIGIN      
        1 gacgccctgc cgcccgccgt gccggccacg gtgagcccga gcatgtcctc acccttgctc
       61 gggttcccgc cggtgatcag catccccacg aggtcgggat actcgaccct gggcggctcg
      121 tagatgcgga tctcgtgctc ccacttgatg ccctgccagg tctgtgaggt catgtggatg
      181 ctgtggatct tcccgagcag agcggtctcc gtgcctgtga actcgtaggc gaagctgtcg
      241 tcgggcagcg cgatgtagtc gaacaagtcg gcgtgggctg ccgaagaagc gatcaggaga
      301 accgtgatgg cgtatcggtg cacttcggtt cctccaagct ctgtgtcacg gcgcaggcgg
      361 cgaaaaggcc gctccaccac tgttcgcccc gtggcccggg ttgcctccca gacagctcgc
      421 tgtgacggag accgtagagc ccgcccggcg gctggggccc ccgcacccag ggccaatgcg
      481 ttcacaagtg gcggtcagat gacacccttc tcgcttcgcg gacctttccc attcgggcct
      541 tcttcagtgg ctcgaagggt ctatggaggc ccgcatgggg ggccagccgt aggctggtgg
      601 tgccccatcg ggcccgagac cgtcggtgcc tcaggatgcc tgcggcctgt gtgccatctt
      661 gtccgaatgg gcaccctaaa ggatgacacc ctggggacgc gccatgcgtt atcgaagata
      721 ggacatgctg gaggcgggtg caggaggtga cgaacatgtc caagcagcgc tacaccgtcg
      781 cgaacctcaa gcccaaggag gggcccacga ctcgtagggg ggacgacacg gtccgcggtg
      841 gccggaccga ctggtatggc aggcgggcgg gctacgattc actggaagcc ctcatcgccg
      901 gcgactgccg tggtccgggg acgatccggg gacggattgt ggcatagacg cggtcgcaga
      961 ccggccgccg gaccgtctcg gccgtccccc aggccccggt gtgcgtacag ggcagctgcg
     1021 gtacatcgtg cgcatgctat cctggccgca agcggctcgt gccgtcagcc gtaccccggg
     1081 ggtggatcac ttctgaagcc gcactggtcc atgtctggcc tgcccgatgt ggtgcagggg
     1141 taccttcgcc gccggggatg ggagcgccgg gctcgcgagg cggccgttca cgtcatctgg
     1201 gagcaggtcg tcggagagca gatcgccgat cacaccgaga ccatcttctg ccgcgacggc
     1261 aagctggtgg tcacagcgtc ggacccaagc tgggcccacg ccatctccgc tttgaggcac
     1321 caactcgcca ccaagctgaa tgaggcgctc ggcgagccga cgatccgcga cattcacctc
     1381 cagtccaagg gcctgggtgt ggccacacgg ggcagcgccc ccatccggcg gcgcgacctc
     1441 gagttcgccg gtggcatgaa ggtcacggat gaggagctcg aagccacgcc aatcagcccc
     1501 gaagtccgat cgcgaatcga gtccaccgtt cgtggcctcc gccaccccga gctgcgcgac
     1561 atggtctatg ccgccctcct tcgcgagagc cgtatcagcg cgtggaagcg cgcgcacggg
     1621 taccgcgagt gcccacggtg cgccgctctc cacaacgatg cccacgagct ctgtggtgac
     1681 tgccgcatcc tcccgcgcta gtggggcgcg aaccccacag tgcctccaga gcgtctaagc
     1741 ctcagtggcg cgatggagcc cggcccgcct tgctgaatcg agggaacagg ggtagactct
     1801 ttggtcggaa acatacaccg gtttcgaacg catgttccgc cacggcccgg cgccggtgcg
     1861 ccgggcgctg tctcgggcgg cccaccccaa ggagcgtggg cgcctctcat cggaaccatc
     1921 cccggaggag gctgtttgat gaaccgcctc aagcccctct tcgcgttctc cgtgctggcc
     1981 gcggtggtcg gtctcgcgcg gagcgcagag gctcaggcgc ccccgacggt ccgccagagg
     2041 gtgacgattg cccagatcgt gtcgctcacc gatgggctgc cctacaaggc aacggccatc
     2101 gcgctggagg acaccctggc actgaaactg cggcacaccg gccgctgcga ggtcaccgac
     2161 cgccagcagt tcctccgggc cgccagggct cagaacctgc cggtcaacga cccggccgag
     2221 attcgtgctg cactggttga gctcggccag cactacggga tcctgggaac ggtgacggcc
     2281 gagggcactc tcgtcaatgc ccagatctcg atcgtggtgg tcaagcccgc cgaacgaacc
     2341 acggcggttc tcacgaactt ggagatcaag ggcggcacgg tgggcatgct cggcttcatg
     2401 tccgcggtcg ccgagcgagt cgccaccgag atgcagatcc cgatggacga cgcgacccgt
     2461 gccgtgatga acagtgcgtc a
//
LOCUS       DAOSPL010000660         2480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42164_length_2480_cov_4.139381, whole genome shotgun sequence.
ACCESSION   DAOSPL010000660 DAOSPL010000000
VERSION     DAOSPL010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42164_length_2480_cov_4.139381"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..994
                     /locus_tag="PLQ54_12035"
     CDS             116..994
                     /locus_tag="PLQ54_12035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS94037.1"
                     /translation="MSDSRVEDSSLPDAPGNIGDAERAAWDQRVEDSHAAKELENLDP
                     EDLQYSVWYTRFPERRWLRARMGVDLTGKRVLDCGCGVGAESLRFASAGASVVGVDIS
                     PASVEIARTYAKRYAPDADIEFIASPIEEVEWPENHFDLIYGASILHHLPDLALAARH
                     LKRVLKPGGLCAFVEPLSANPILRLARNDRVWWLRYPGKAHTPGERPLSYRDYNVFVD
                     EFDEHIYYEYGLSGQCLRLIPSGFWGKGPAGRRRYILARKTFSSIDAVLRAFPPFRPL
                     CRFVAILGMKNADGTP"
     gene            complement(1117..>2480)
                     /locus_tag="PLQ54_12040"
     CDS             complement(1117..>2480)
                     /locus_tag="PLQ54_12040"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOS94038.1"
                     /translation="GYDWPTYAGGNDGGLSMGPYYGLDGTALKWEAFDAMKKLGYADL
                     YSIPFVQNLGDFYLYMMPPNARFFGTFGDGGATVHINSCAMLERWDMLWLSQVYQNPG
                     WRWWVANKKIEASDPPADPLDPSGVQWNYQILLLGLREAKTPVAASPPTVPNSKLFPE
                     SGFVSMHTDIANPAEDTILNMRCAPYPRGSVHHSQPDQNAFVLYHHGAPLAINAAYYG
                     GLADVDFGDPYSVYWGRQTKSKNAILIDNVGQRPDRATSAGEILQFDSTADYDYFVGD
                     AVQAYNLNQSGIANAVRRHAAMVHKPGEAPYVVMVDEVRMPAAHNIQWLLHTMSQAVV
                     DAANQKIILTYGNVHAQVRLFAPQALTISQTNLTDPLLTVDTARLGEHWHLTCATPSA
                     ATTFLIGSVFFPYVDGEEGAIPSISGHANGDNSQFTFTVGADTVNVDVASMAMSIVSG
                     QTP"
BASE COUNT          440 a          765 c          824 g          451 t
ORIGIN      
        1 actatcctcg agttggcgcg cgggcgcacg tgatcggcgc ccgggcgcgg gggggagagg
       61 tcgacggagc gagaaggcct cgcacgagga gcagaaggag agggcatggt gtcgtatgag
      121 tgactctcgg gtcgaagata gctcactgcc ggatgcgccg gggaacatcg gggatgccga
      181 gcgggctgcc tgggatcagc gggtcgaaga ctcccatgcc gcgaaggaac tcgagaacct
      241 ggaccctgag gacctacagt attcagtctg gtacacccgg ttccccgagc ggcgctggct
      301 gcgcgcccga atgggcgtcg atctcaccgg gaaacgcgta ctcgactgcg ggtgcggagt
      361 cggtgcggag agtctgcgct tcgcgtcggc gggggccagt gttgtgggcg tcgatatctc
      421 gcccgcctcc gtcgagattg cgcggaccta tgccaaacgt tacgcgccgg acgcggacat
      481 tgagttcatc gccagcccca tcgaggaagt ggagtggccc gagaatcact tcgacctgat
      541 ctatggggcc agcatcctcc accatctgcc cgacctggct ctggcggccc ggcacctcaa
      601 acgagtgctc aagccgggag gcctctgcgc gttcgtcgag ccactcagcg cgaacccgat
      661 actgcgcctg gcccgcaacg accgcgtgtg gtggctgcgc tacccgggaa aggcgcacac
      721 gcccggcgag cgcccgctgt cctatcgcga ctacaacgtg ttcgtcgacg agttcgatga
      781 gcacatctac tacgagtacg gcctctccgg ccagtgcctg aggctcatcc catccgggtt
      841 ctggggcaag gggcccgccg gccgacgccg gtacatactg gcacgcaaga ccttctcgag
      901 catcgacgca gtactccgag cctttcctcc gttccggccg ttgtgccgct tcgtcgccat
      961 tctggggatg aagaacgcgg acggtactcc gtaatgccat cggtgtttcg catcatcctc
     1021 aaagcggtgc ggaccagcac gtagaacaag gggcgccgcg gggtctctct ctccccgcgg
     1081 cgccccggct tcttccggtc agaacggcaa gcctacctat ggagtctgtc cgctcacaat
     1141 cgacatggcc atcgaggcca catcgacatt gacggtgtca gctcccaccg tgaacgtgaa
     1201 ctgggagttg tctccgttag cgtggcctga gatggacggg atcgcgcctt cctcgccgtc
     1261 cacatagggg aagaacacgc tgccgatcaa gaacgtggtg gccgcactgg gcgtcgcgca
     1321 ggtcaggtgc cagtgttccc cgagcctcgc cgtgtccaca gtcagcaacg ggtcggtcag
     1381 gttggtctgc gagatggtga gtgcctgcgg cgcgaaaagc ctaacctgag cgtgcacgtt
     1441 accgtaggtc aggatgatct tctggttcgc ggcgtccacc acggcctgag acatggtgtg
     1501 gagaagccac tggatgttgt gcgcggccgg catccgcacc tcgtccacca tgacgacgta
     1561 gggcgcctcg ccgggcttgt ggaccatggc cgcgtgacga cgaaccgcgt tggcgatacc
     1621 cgactggttc aggttgtacg cctggacggc gtcgccgacg aagtagtcgt aatcggccgt
     1681 gctgtcgaac tgcaggatct cgcccgccga agtcgcgcgg tcgggtctct ggccgacgtt
     1741 gtcgatcagg atggcgttct tggacttggt ctgccgaccc cagtagacgc tatagggatc
     1801 gccgaagtcc acatcagcca ggccgccata gtaggccgcg ttgatggcca gtggcgcgcc
     1861 atggtgatag agcacgaagg cattctgatc gggctgcgaa tggtgcaccg agcccctcgg
     1921 atagggcgcg caccgcatat tgaggatggt gtcctccgcg gggttcgcga tatcggtgtg
     1981 catcgagacg aagccggact ccgggaatag cttggagttc gggaccgtgg gcggcgacgc
     2041 agctacgggc gtcttagcct cccgcagtcc caacaggagg atctggtagt tccactggac
     2101 gccgcttggg tccaacgggt cggctggggg atcgctggcc tcgatcttct tgttggccac
     2161 ccaccagcgc catcccgggt tctggtagac ctggctcagc cagagcatgt cccaccgctc
     2221 cagcatggca caggagttga tgtggaccgt cgcgccgcca tccccgaatg tgccgaagaa
     2281 gcgggcattg ggcggcatca tgtacaggta gaaatcgccc aggttctgga cgaaggggat
     2341 gctgtaaagg tcagcgtacc cgagcttctt catggcgtcg aaggcttccc acttgagtgc
     2401 ggtcccatcg agcccatagt aggggcccat cgagagacca ccatcattgc cgccggcata
     2461 ggtgggccag tcgtagccgc
//
LOCUS       DAOSPL010000661         2480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42173_length_2480_cov_2.775670, whole genome shotgun sequence.
ACCESSION   DAOSPL010000661 DAOSPL010000000
VERSION     DAOSPL010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42173_length_2480_cov_2.775670"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PLQ54_12045"
     CDS             <1..588
                     /locus_tag="PLQ54_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOS94039.1"
                     /translation="GSVDVVLMQLGLPVSTVDQGIRLCQEHGVPVYLDPTPIRRSLPE
                     LWCGVALIAPNETESQILLGGSRTDGPEEARTAAARLRDAGVGVAVVKLGAAGCVVAA
                     DGWTAHVPGVKVDVVDTTAAGDAFMAGLAVARGEGADWVEAARFANCAGALAATKLGA
                     QPSLPYRPEVDALYRSGDAGVRHCAPTLGEREGGG"
     gene            747..2084
                     /locus_tag="PLQ54_12050"
     CDS             747..2084
                     /locus_tag="PLQ54_12050"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOS94040.1"
                     /translation="MRYGIQDSAIEELLLRSRSSSEPVTGDVAFGAPMVPAGDTTFEY
                     LIDMTDSGNWVNDEQIVSPLSGSDTGARHAPSADETEARTDYHFPAPIPVVTPGTVIG
                     VRRPGSPGQAGMTVLGQPVEVPPAKDETPRIGAGVTLVEKPDGTAEVVAQVAGKAVLD
                     GNRLVVSSEHTVMGDVSVRGGDVIFPGDVVVRGNVLDGAVVKAGGNITVMGRVDGAHL
                     EAEGTIAVSKGIVGGGRGMVRARGDITATFCEGATVESRGDVDISRGIMNSDVSASGT
                     VRCMQGRGAIIGGTIRAGRAVEAKNIGTESSSETQIIVGTDFGLLRERDDVLRTKEQC
                     ESLLARLDVALGPLSETSDLSHLPEDRREMVRKALQERVRQRARLSQIQTRLEDLERQ
                     MAATDQGAVKVFGCAQPGTRIIIRKCQVLLTDTVNYAQLVENQEMGTIRSAPL"
     gene            complement(2079..>2480)
                     /locus_tag="PLQ54_12055"
     CDS             complement(2079..>2480)
                     /locus_tag="PLQ54_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOS94041.1"
                     /translation="GGAGGQHVNKTDSAVRITHIPTGTVVQCQNERSQHRNRDTAMRI
                     LRSRLFEQERRRQEEEKAALRGEQRTIGWGSQIRSYVLHPYMMIKDLRTGYTSSDTQR
                     VLDGAIDPYIQAYLQSTAAGSVSAGGSGAPL"
BASE COUNT          432 a          756 c          900 g          392 t
ORIGIN      
        1 gggtcggtcg acgtcgtgct catgcagctt ggactgccgg tttcgactgt cgatcagggg
       61 atcaggttgt gccaggagca tggcgttccg gtatacctgg atcccacgcc tatacggcgc
      121 tccctgcccg agctgtggtg cggtgtggct ctgatcgcgc ccaacgagac cgagtcccag
      181 attctgctgg gtggaagccg gactgacggc ccggaggagg ctcggacggc ggccgcccgg
      241 ctacgggacg ccggtgtcgg cgtggcggtc gtgaagctgg gcgccgccgg atgcgtggtc
      301 gcggcggatg ggtggacggc gcacgtgccc ggggtcaagg tagatgtggt ggacaccacc
      361 gcggctggcg acgcctttat ggccgggctg gccgtggcgc ggggcgaggg ggcagactgg
      421 gtcgaggcgg cccgattcgc caactgtgcc ggggcgctgg ccgccacgaa gctgggtgcc
      481 cagccatcat tgccataccg gcccgaggtg gatgcgctct accggagtgg tgatgctgga
      541 gtgcggcatt gcgcgccgac attgggggaa cgggaagggg gaggatgatg gccggagtgg
      601 gcgacgcgtc tgaagatatg aatggtgatg caaccgttct aatagcgcga caccgcatga
      661 ctgcggcggt cacgatcact ccccccgtgg gcagaggggc gcccgttctc gcgccgggaa
      721 tacgccagcg actcgccgcg gcgggcgtga gatacgggat ccaggactcg gccatcgagg
      781 agctcctgct gcgcagccga tcgtcttccg agccggtgac gggcgatgtt gctttcggcg
      841 cgccaatggt gccggcgggc gacaccacct tcgagtacct catcgacatg acggatagcg
      901 gcaactgggt caacgacgaa cagatcgtca gcccgctatc cggttccgac accggcgcgc
      961 gccacgcgcc gagtgccgac gagaccgagg caagaaccga ctatcacttc ccggccccca
     1021 ttcccgttgt gactccggga acggtcatcg gcgtccggcg gcccgggagt cccggccaag
     1081 cgggcatgac ggtgctgggc caacccgtgg aggtgccgcc tgccaaggac gagacaccgc
     1141 gaatcggagc gggcgtcaca ctggtagaga agcccgacgg gaccgccgag gttgtggcgc
     1201 aggtggcggg caaggcggtc ctcgacggga acaggctggt tgtgtcgtcg gagcacacgg
     1261 tgatgggcga tgtctcggtc cggggcggag acgtgatctt cccgggcgac gtcgtggtgc
     1321 ggggcaacgt cctggacggc gcggtggtga aagcgggagg caacatcacg gtcatgggcc
     1381 gcgtcgatgg cgcccacctg gaggccgagg gcacgattgc ggtgagtaag ggaatcgtgg
     1441 gtggaggacg cggaatggtc cgggcccgcg gggacatcac cgcaacgttc tgtgagggcg
     1501 caacggtcga gtcgcgaggc gacgtggaca tcagccgcgg tatcatgaac tctgacgtct
     1561 cggcctcagg cacagtccgc tgcatgcagg gccggggagc catcatcgga ggcaccatcc
     1621 gggcgggcag agccgtcgaa gccaagaaca tcgggaccga atcgagcagc gagacccaga
     1681 ttatcgttgg cacggacttc ggcttgcttc gcgaacggga cgatgtactg cgaacgaagg
     1741 agcagtgcga gtcactcctg gcccggctgg acgtggcact cggccccctg tcagagacga
     1801 gcgacctaag ccacctgccc gaggaccggc gcgagatggt ccggaaggcg ctgcaggagc
     1861 gagtgagaca gcgcgctcgg ttgtcacaga tccagacccg cctcgaggac ctcgaacgcc
     1921 agatggctgc caccgaccag ggagcggtga aggtgtttgg ctgcgcgcag ccaggcacac
     1981 ggatcatcat ccgaaagtgc caagtcctgc tgacggacac ggtcaactac gcccagctcg
     2041 tggagaacca ggagatgggc accatacgat cggcgccgct atagcggggc gccagagcca
     2101 ccggctgaga ccgagcccgc ggccgtgctc tgcagatatg cctggatata cgggtcgatg
     2161 gccccatcca gcactcgttg cgtatcgctg ctcgtgtacc ccgtgcgcag gtccttgatc
     2221 atcatatacg ggtgcaacac gtatgagcgg atctggctgc cccacccgat ggtccgctgc
     2281 tcaccacgca gggctgcctt ctcctcctcc tgacggcggc gttcctgctc gaagagcctg
     2341 gaccggagaa tcctcatcgc cgtatcgcga ttccggtgct gggaacgctc attctgacac
     2401 tgcacgacgg tgccggtcgg gatgtgagtg atgcggacgg cggaatccgt cttattgacg
     2461 tgctggcccc ccgcgccccc
//
LOCUS       DAOSPL010000662         2480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42174_length_2480_cov_2.737732, whole genome shotgun sequence.
ACCESSION   DAOSPL010000662 DAOSPL010000000
VERSION     DAOSPL010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42174_length_2480_cov_2.737732"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1909)
                     /locus_tag="PLQ54_12060"
     CDS             complement(<1..1909)
                     /locus_tag="PLQ54_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94042.1"
                     /translation="MYRTDLGNVRPALAWALAVLAVSAATSAQEGRPDRPNAPSPFVT
                     AAAVLSEAPAPRPGAIVADPRAARVEAWLAQEDYSELAQALVALGVEVALFQPGGQVK
                     AADGTVALSGYAPDRARALAKFLGEAERAGLQVMLGPVDVSLSGATALAKALASAERA
                     VPAQGSLAGLCLRLPALPAPQPAATPEDSDAALASWQTQVRSLVGEAARASGPLGRRV
                     YVLSDSPELLGLALRDAPEGVSPMFHAELGREPTLGYGYRVAAVSGSLEPPGVWLDSP
                     TASAHAMVRAMALACLRGARLLCLGDARYLLGEALDGRYVADMAASLKEFASLPDRKA
                     RTGVALVVDAEAQARGLLGPASGLPSETPSNLAAAMALNSSGYPWSLVTDPTVLEALD
                     PDAVVVPAGAAMPEQAIAALEAYTRGGGRLVCFAEFGARQLSGEDRLKADRLGVDLML
                     PAARVRRVRHPLTTLRMAAAIPGARHAKGDEIPVRPGEGQSVFLPEAHSGSETLALYP
                     ALGAPAVLAWALDAGRVAWVNGSPEMLGGNLLADVLAHLGVSPVVPDRGGDLRAPTNL
                     DGMHAEQRGRVLDYRTVALNPEWTPLPAAPDDGSMYDMYLAGPNLTAVSLGYRHENLA
                     DGSVIGIGMSDI"
     gene            complement(1977..2285)
                     /locus_tag="PLQ54_12065"
     CDS             complement(1977..2285)
                     /locus_tag="PLQ54_12065"
                     /inference="COORDINATES: protein motif:HMM:NF016798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUN14 domain-containing protein"
                     /protein_id="HOS94043.1"
                     /translation="MNPGALVEQFGVGGLFGIAVGYTAKKATKAMAILLGVLFICIQL
                     LAHYHLITPHWDKVGDLAKHATAPEATSPLRSLMSLLTANLPFGAAFMVGFTWGFRRG
                     "
BASE COUNT          404 a          875 c          877 g          324 t
ORIGIN      
        1 cgatgtccga catgccgatg ccgatgaccg atccgtcggc gagattctca tgacggtacc
       61 cgagcgacac ggcggttagg ttgggccccg ccaggtacat atcgtacatg gagccgtcgt
      121 cgggggccgc gggaagtggg gtccactccg gattgagtgc cacggtgcgg tagtcgagca
      181 cccggccgcg ctgctcggcg tgcataccgt cgagattcgt cggcgcccgc aggtcaccgc
      241 cacggtcggg aaccacgggt gagacaccca gatgcgccaa cacgtcggcc agcaggttgc
      301 ccccgagcat ctccggagaa ccgttgaccc acgccacccg gccggcatcc agcgcccacg
      361 caagaaccgc tggcgcgccc agagcagggt acagggccag cgtttccgat ccgctgtggg
      421 cctcgggaag gaacacactc tggccctcac cggggcgaac cggaatctcg tctcccttgg
      481 cgtgacgggc cccggggatg gcggctgcca tgcggagggt ggtcagcggg tgccggacac
      541 gccggacgcg ggctgcgggc agcatcaggt cgacacccag tcgatccgcc ttcagtcgat
      601 cctctcccga cagctggcgc gcgccgaact cggcaaagca tacgagccgg cccccgccgc
      661 gggtgtacgc ttccagagcc gcgatggctt gctccggcat cgccgcgccg gcaggaacga
      721 ccaccgcgtc ggggtccagc gcttcgagca ccgtgggatc ggtcaccagc gaccacgggt
      781 aaccggatga gttgagagcc attgccgcgg ccaggttcga cggggtctcc gacgggagtc
      841 cgctagcggg acccagtaga ccgcgggcct gggcctcggc atcgacgacc agagcgacgc
      901 ccgtccgggc cttgcggtcc ggcagtgagg caaactcctt gagcgacgcc gccatgtccg
      961 ccacataccg accgtccagc gcctcgccca gcagatagcg cgcgtcgccc aggcacagca
     1021 gtcgagcgcc acggaggcac gccagggcca tggcccggac catggcgtgc gcgctggccg
     1081 tgggactgtc gagccatacg cccggcggct ccaggctacc cgagaccgcc gccactcgat
     1141 agccgtaccc cagcgtcggc tcacgcccca actcggcgtg gaacatcgga gagacgcctt
     1201 cgggcgcatc gcgcaacgcc aaaccgagca gctcggggga gtcgctgagc acgtagaccc
     1261 gacggcccag cggcccagag gcccgcgcgg cctcccccac cagcgagcgc acttgcgtct
     1321 gccaggaggc cagggcggcg tccgagtcct ccggcgtggc ggccggctgg ggagcaggga
     1381 gggcgggtag gcgaaggcac aggcccgcga ggctgccctg agctggcacc gcccgctcgg
     1441 ccgacgccag ggctttggcc agtgcggttg cgccggagag agagacgtca acggggccga
     1501 gcatgacctg cagccccgcg cgctcggctt cgcccaggaa cttggccagc gccctggccc
     1561 ggtccggcgc gtaaccggac agcgccacgg tcccgtccgc cgccttcacc tggccgcccg
     1621 gctggaacag cgccacctca acaccaaggg ccacgagtgc ctgcgcgagt tcgctgtagt
     1681 cctcctgcgc cagccatgcc tcgactcggg cagctcgggg gtcggccaca atggcgccgg
     1741 ggcgcggcgc gggcgcctcc gaaagaacgg cggctgcggt gacgaagggt gagggcgcgt
     1801 ttgggcggtc cggccggccc tcctgcgccg aggtagctgc ggacacggcc aagacggcga
     1861 gcgcccacgc cagggctggc cgcacgttcc cgagatcggt gcggtacatg cacagagccc
     1921 ccttcgagcg aaggagtgag accatgatcg gcgccgaggc cggcctcgct ccgggcctag
     1981 cctcggcgga acccccatgt gaaccccacc atgaaggcgg ccccaaatgg caggttggcg
     2041 gtgagcaggg acatgaggct gcgcagaggc gacgtcgcct ccggggccgt cgcgtgcttg
     2101 gccaggtctc ccaccttgtc ccagtgcggg gtgatgaggt gatagtgcgc cagaagctgg
     2161 atgcagatga agagcacgcc cagcaggatc gccatggcct tcgtagcctt cttggccgtg
     2221 taccccacgg caattccaaa caggccgccg acgccgaact gctccaccag cgcgccaggg
     2281 ttcacgatga tcacctaccc gaacgggcgc ggcccccagg ggtgccgcgc ctgctgcagg
     2341 ctgaactccg cgccaacggc ttgggttcgg gttccagtga gcggtgcgcg gagggagacc
     2401 gggcattggc ggtaacatag ggctgttggc cgggcggctg ggtctcgcga aggtcgcgcg
     2461 gaacgcccgc ctggtctttc
//
LOCUS       DAOSPL010000663         2478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42228_length_2478_cov_3.048287, whole genome shotgun sequence.
ACCESSION   DAOSPL010000663 DAOSPL010000000
VERSION     DAOSPL010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42228_length_2478_cov_3.048287"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="PLQ54_12070"
     CDS             <1..166
                     /locus_tag="PLQ54_12070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOS94044.1"
                     /translation="RATLYAHLSSISVRVGQTVTAGQKLGEVGSTGRSTGPHLHFEVR
                     INGRPTNPMM"
     gene            282..1652
                     /locus_tag="PLQ54_12075"
     CDS             282..1652
                     /locus_tag="PLQ54_12075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOS94045.1"
                     /translation="MRRSSQLGRTLLVVTMVFWSFAAGWAFQRYWGPGGSTSGQYAAL
                     AQAGESRAGGDLLGQSDGSGLEPLGLFWEVLNHVRHDYYKPVTDEDEKALAYGAAQGL
                     TRALGDPYSDFMVPQVAQEFSADTEGVMEGIGAELINQVDDSSGQRRVVIHKPLKGSP
                     AAKAGLLPEDQIVFVDDVSVIDRSVDEVAVRIRGERGTTVKITVYRKGSDDLLDFTIT
                     RQRIELPVVDYREVADGIWLLAIDEFNDLTVSKTLEALDDIDKQGRKGLIIDLRDNKG
                     GVLDFCVDISSVFIKDQPIVWVEERNGAQKAMPANPRVYRGEPGPMVVLVNGRTASAA
                     EIVAGALQDCGVAKLVGTRTFGKGLVQTVIPLHDPSVKLKLTTAKWLTPKKRFINREL
                     PTVMKRLTPGAAPDVEDSKRGGLAPDIEVKITPEDDEKRVLERLPDENDPQLQAAINA
                     LKKSEA"
     gene            1737..>2478
                     /locus_tag="PLQ54_12080"
     CDS             1737..>2478
                     /locus_tag="PLQ54_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94046.1"
                     /translation="MDQDGYDLRPTSATSAAVDRPARRFRRQLIIERAIGIPLIIVLV
                     YWVAALKSAGGRACFIEVGEDDAQIRIAVDGEDSAERVLTRLRQSYGGSLPPDSVQFR
                     PKPSLHMARLGRERLLTVDEAVKTLGQAIESGQIEVVTKAAVVYVNGRPLLAVRSPAE
                     VDAVMEGLKKRAIEDIAPENVVEQPRILEKITYQEETRAPKSIMTSREEAEKRLLDVA
                     QKAVEDWVGPDDTLERYLAKYSISRARLQ"
BASE COUNT          496 a          769 c          823 g          390 t
ORIGIN      
        1 ccgggcaacc ctgtacgccc atctatcgtc tattagcgtg agagtgggac agaccgttac
       61 cgccgggcag aagctaggcg aagtgggaag cacgggccgc agcacggggc cacatctcca
      121 cttcgaggtg cgtatcaacg ggcggcccac caaccccatg atgtaaccag agcgacctgc
      181 ggcacccagc gtgccgcagg gatatagcag accggaactc cgcggcgtga gcccaagcgt
      241 caacgctcgg agtgtacccg tggcgggggc gtggttttgc aatgaggcga tcgagtcagc
      301 tgggccggac actcctggtg gtaaccatgg tgttctggtc ctttgccgcc ggatgggctt
      361 tccagcgata ctggggacca ggcggcagca cctcgggcca gtacgcggca ttggcccagg
      421 ccggagagag cagggcaggc ggcgatctgc tgggacagag cgatggttcg ggacttgagc
      481 ccctcgggct gttctgggaa gtcctgaacc acgtccggca tgactactac aagccggtta
      541 ccgacgaaga cgaaaaggcc ctcgcctacg gcgccgccca gggactcacc cgcgccctgg
      601 gcgatcccta ctcggacttc atggtgcccc aagtcgcgca ggagttctcg gccgacacgg
      661 agggtgtgat ggaagggatc ggggccgagc tcatcaacca ggtcgatgac agctcgggtc
      721 aacgccgggt cgtcatccac aagcctctca agggcagtcc ggcggccaaa gctggcctac
      781 ttcccgagga tcagatcgtg ttcgtggacg atgtgtccgt cattgatcgg tcggtcgatg
      841 aggtcgccgt caggatccgt ggggaacggg gcacgaccgt caagatcacg gtctaccgca
      901 agggttcgga tgaccttcta gacttcacca tcacacgcca gcgcatcgaa ctgcccgttg
      961 tcgattaccg cgaggtcgcc gacggcatct ggctcctcgc catcgacgag ttcaatgacc
     1021 tcacggtgag caagaccctc gaggcgctgg atgatatcga caagcaggga cgcaaaggcc
     1081 tgatcatcga cctgcgcgac aacaagggcg gcgtcctcga cttctgcgtc gatatcagca
     1141 gcgtcttcat caaagaccag cccatcgtgt gggtcgaaga gcggaatggt gcacagaagg
     1201 ccatgccggc gaatccgagg gtctaccggg gcgagcccgg gcctatggtg gtactcgtta
     1261 acggccggac ggccagtgcg gcagagatcg tggcgggtgc cctgcaggac tgcggcgtgg
     1321 cgaagctggt ggggacccgg accttcggca agggtctcgt ccagaccgtg attccactcc
     1381 acgatccgtc agtgaagctc aagctcacga cggcgaagtg gctcacaccc aagaagcggt
     1441 tcatcaaccg cgagctgcca acggtgatga agcgactcac gcccggcgcc gcgccggacg
     1501 tggaggacag caagcgcggc ggccttgcac cggatatcga ggtgaagatc acgccggagg
     1561 atgacgagaa gcgggtactg gaacgtctgc cagacgagaa cgatcctcag ctgcaggcgg
     1621 cgatcaatgc tctgaagaag tctgaggcct aggccgggcg agccagtccc ccggcagggg
     1681 gaccaacttc gcgtagaggg cgcgcgggcg gcaccaaggc ccgcgcgtgg tgttccatgg
     1741 accaggatgg ctacgacctc agacccacct ccgccacgtc ggctgcggtg gatcggccgg
     1801 cgcgacggtt ccggcgccaa ctgatcattg agcgcgccat cggcattcct ctgatcatcg
     1861 tgctggtcta ctgggtcgcg gctctcaaga gcgccggtgg acgggcgtgt ttcatcgagg
     1921 tgggcgagga tgacgcccag atccgcatcg cggtcgatgg cgaggactca gccgaaagag
     1981 tgctgactcg cctccgccag tcctatggtg gcagcctccc ccccgactcg gtgcagttcc
     2041 ggcccaagcc ctcgctacac atggcccgcc tgggccggga gcgtctcctc acggtcgacg
     2101 aggcggtcaa gactctcggc caagccatcg aaagtggaca gatcgaggtc gtcaccaaag
     2161 ctgccgtcgt gtacgtcaac ggcaggccac tcctcgcggt acgcagcccc gctgaggtcg
     2221 atgcggtcat ggaaggactc aagaagcggg ccatcgagga cattgccccg gagaacgtgg
     2281 tcgagcagcc caggatcctc gagaagatca cataccagga ggagacgcgg gctcccaagt
     2341 cgatcatgac aagccgcgag gaggccgaaa agcgcctgct cgacgtggca cagaaggccg
     2401 tcgaggactg ggtggggccc gacgacactc tcgaacgcta cctcgccaag tactcgatct
     2461 cgcgcgcccg acttcagg
//
LOCUS       DAOSPL010000664         2477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42251_length_2477_cov_3.058629, whole genome shotgun sequence.
ACCESSION   DAOSPL010000664 DAOSPL010000000
VERSION     DAOSPL010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42251_length_2477_cov_3.058629"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLQ54_12085"
     CDS             <1..291
                     /locus_tag="PLQ54_12085"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOS94047.1"
                     /translation="PDGQGHVGGFHGPRWADGAFPSGHTAMMFSTATVVGKQYRKWKW
                     WAYGLAAGTAISRVLLDEHHPSDALAGAILGVGIGNFVMRNKGWIARISIKF"
     gene            329..1549
                     /gene="tyrS"
                     /locus_tag="PLQ54_12090"
     CDS             329..1549
                     /gene="tyrS"
                     /locus_tag="PLQ54_12090"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOS94048.1"
                     /translation="MSVEDQLELIRRGAVLVENEEELRAKLALGRPLRIKHGADASSP
                     RLHLGHAVQLRKLRQLQEMGHEVIFLVGDFTARIGDPSGKSQTRPLLSAEQVEANAAR
                     FAAQAAKILDMDRARVEFNSRWLGQMTFADVINLASRATVARMLERDDFAIRFAENRP
                     ISVLELLYPLVQGYDSVALEAYIETGGTDQTFNFYQARTLMRSYGLEPQCFITMPLLE
                     GTDGHEKMGKSLGNTIDLLDTPAEKFGKVMSLPDTLLTKYLLLCTDTPEVEIEELGRG
                     LADGTVHPGQLKRRLAREIITLYDSAEEAREAEREFDIRHKPGTDVSALERVAEEVRL
                     PARVFAEPMWIVGLLSACGLVKTNSEARRMVQQGAVRIDGERVEDEAAEVLVREGLIV
                     AVGKRRVARIVREA"
     gene            1569..>2477
                     /locus_tag="PLQ54_12095"
     CDS             1569..>2477
                     /locus_tag="PLQ54_12095"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOS94049.1"
                     /translation="MSEGDPTRAEREIEQLGQRAKAAARWLALAPSGQKDEALRAAAE
                     ALVRSTDVVLEANAADVTSARERSLRESLIDRLALSPERIRAMAQALVDITTLRDPVG
                     VVRAGRRLPNGLDLQQVTVPIGVIGMIYESRPNVTVDAIGLCLKSGNAVILRSGSDAI
                     RSCTALVDALRPAIESTGLPADVAQIVRSTDRASATALMRCNRYLDLLVPRGGAGLIQ
                     TVVEQSTVPVIETGVGNCHTFVDASADVDMAVRIAVNAKTQRPGVCNAMETLLVHSAI
                     AEAFLPRVVREMTLRQCEVRGCGRTRA"
BASE COUNT          420 a          746 c          903 g          408 t
ORIGIN      
        1 cccgatggcc aaggccatgt ggggggcttc cacggacccc ggtgggccga cggcgcgttc
       61 ccatccggcc acacggcgat gatgttctcg accgcgaccg tggtgggcaa gcagtaccgc
      121 aagtggaagt ggtgggccta cgggttggcg gcgggtacgg ccatcagccg ggtacttctg
      181 gatgaacacc atccatcgga cgcgctcgcc ggagcgatcc tcggcgtggg catcggcaat
      241 ttcgtcatgc gcaacaaggg atggatcgcg cgcatctcca tcaagttctg actgcccccg
      301 cgatgagggg ccgactggga ggccatgcat gtcagtcgag gaccagctcg agctgatcag
      361 acggggcgcc gtgctggtcg agaacgagga ggagctgcgg gccaagcttg cattgggccg
      421 tccgcttcgc atcaagcacg gcgccgacgc atcgagcccg cgccttcacc tggggcacgc
      481 ggtgcagcta cggaagctcc gacagcttca ggagatgggt catgaggtca tcttccttgt
      541 cggcgacttc accgcccgga ttggcgaccc gagtggcaag tcgcagacga ggcccctgct
      601 ttcggcggag caggttgagg ccaacgcagc ccgttttgcg gcccaggctg ccaagatcct
      661 cgacatggat cgggcgcgcg tggagttcaa ctcgcggtgg ctcgggcaga tgacgttcgc
      721 ggatgtcatc aacctggcat cccgcgcgac ggtcgcgcgg atgctggagc gggacgattt
      781 cgcgattcgg ttcgcggaga accgccccat cagcgtactc gagctgctct atcccctggt
      841 gcagggctat gactcggtgg cgcttgaggc ctacatcgag accgggggca ccgaccagac
      901 gttcaacttc taccaggccc ggacgctgat gaggtcctat ggcctggagc cccaatgttt
      961 catcaccatg cccctgctcg agggaaccga cgggcacgag aagatgggca agtcgctggg
     1021 gaacaccatc gacctactcg acaccccggc cgagaagttc ggcaaggtga tgtccttgcc
     1081 ggacacgctg ctcaccaagt acctgttgtt gtgcaccgac acgccggagg tcgagatcga
     1141 ggagctcggg cgggggctgg cggatggcac ggtgcacccc gggcaactga agcgccgatt
     1201 ggcccgagag atcatcacgc tgtatgactc ggccgaggag gctcgcgagg cggagcgcga
     1261 gttcgatatc cgccacaagc cgggcacaga cgtctcggct ctggagcggg tggccgagga
     1321 ggttcggcta cctgcacggg tgttcgccga gccgatgtgg atcgtggggc tcctgtccgc
     1381 ctgcggcctg gtgaagacga acagcgaggc gcggcggatg gtgcagcagg gcgcggtacg
     1441 catcgatggc gagcgcgtgg aggatgaagc cgccgaagtt ttggtccgcg agggcctgat
     1501 cgtcgccgtc ggaaagcgac gcgtggcgcg catcgtgcgc gaggcatagc ggggaggtgg
     1561 aagcggcagt gtccgagggc gatcccaccc gggcagagcg ggagatcgag cagttggggc
     1621 agcgggcgaa ggccgcggcg cgttggctgg cgctggcgcc ttcgggccag aaggacgaag
     1681 cactgcgggc cgccgccgag gcgttggtgc ggagcaccga tgtggtactg gaagccaatg
     1741 cggctgatgt gaccagcgcg cgtgagcgaa gcctgcgcga gtcgctgatc gaccggctgg
     1801 cgctgagccc tgagcggatc cgcgccatgg cgcaggcact ggttgacatc accacgctgc
     1861 gggaccccgt gggcgtggtg cgggcgggcc gccgcttgcc caatggtctg gacctgcaac
     1921 aagtcacggt gcccatcggt gtcattggaa tgatctatga gtcgcgcccg aacgtgacgg
     1981 tggacgcgat cgggttgtgc ctgaagtccg gaaatgcagt gatcttgcgg tcgggaagcg
     2041 atgccatacg ttcgtgcacg gccctggtcg acgctctgcg tcctgccatc gagagcacgg
     2101 gactgcccgc cgatgtggcc cagatcgtac gcagcaccga tcgggcctcc gccacggcgc
     2161 tgatgcgctg caatcggtac ctggatctgc tggttcctcg gggcggggcg gggctgatcc
     2221 agaccgtggt cgagcagtcg acggttccgg tcatcgagac cggggtcggc aactgccaca
     2281 cgttcgtgga tgcgtcggcc gatgtcgaca tggctgtgcg catcgcagtg aacgcgaaga
     2341 cacagcggcc cggtgtctgc aacgccatgg agaccctact ggtgcactcg gccatcgccg
     2401 aggcgttcct gccgcgagtg gtgcgtgaga tgacgttgcg gcagtgcgag gtccgtggct
     2461 gcgggcgtac ccgcgcc
//
LOCUS       DAOSPL010000665         2476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42278_length_2476_cov_2.622057, whole genome shotgun sequence.
ACCESSION   DAOSPL010000665 DAOSPL010000000
VERSION     DAOSPL010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42278_length_2476_cov_2.622057"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1874)
                     /locus_tag="PLQ54_12100"
     CDS             complement(<1..1874)
                     /locus_tag="PLQ54_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94050.1"
                     /translation="MVRWTLMAAAAGVAVAGAFAQGAGEDYFIGPQAAPPLEPPAGAT
                     APSASTSSAVDAKAMYIQLVPERDTIVYSGSVEITYQDSVITADNCTFETGKNQAVFT
                     GHVRVQWQDQVIEAVWVWVDFTNATYRAKECRLELPPSFFGSEVSAPIYVWGDRLEGD
                     LEELVIHDAFVTPCDRYRDPHLGASARRVRVYRRGKHVETSAIKLKAKGHTFFWFPGF
                     SIRTRWFTRGDIFPTIGQNSYEGLWGETRYLYNSNGAIDVRVTELQGTKISHEYAYEL
                     GNSGYGSSLITYGTLSRNFTGRLSHSQRFGQSTNLTGSYDLRQSSAAVSTQSTTQAAN
                     LSLSRNVGRTSLTANYTQNTTTTTQTRSNTTAAVTLARELWGETQKANAEAALQYSSY
                     DVGDGLYDRELTANLEFRQPTGVADFDVIYDRRFDLDGTEYTGDDSYSSLDRRPNATA
                     SIDFRDWGLELQSFTSRGKLSLGRFVDTANEIDRLRLAMDLEGSYSPIKIGKSTTATL
                     AGAFQQRIYRSDEALYTLGSQATVRSKFGTAAALDLAWNWKTYDGYTPFRQDYATKQH
                     SVRAALRLTPEQGTQADVSSSYDIQAGRWGDIRMSFNRRKGLSNLTASTGYDLQNHRW
                     R"
     gene            complement(2209..>2476)
                     /locus_tag="PLQ54_12105"
     CDS             complement(2209..>2476)
                     /locus_tag="PLQ54_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HOS94051.1"
                     /translation="TYPYGVGPRLDDIGAQISVRRIDGLIHYVQSFCFRQIADLLLRR
                     HLPVPILTLEGDRPGPVDGATRIRIETFVDMLGVRRPPKEGRWR"
BASE COUNT          391 a          783 c          840 g          462 t
ORIGIN      
        1 cgccaccggt ggttctggag gtcatagccc gtactggccg tgagattgga cagccccttc
       61 cggcggttga atgacattcg gatgtcgccc cagcggccgg cctggatgtc gtaggacgag
      121 gacacgtccg cctgtgtgcc ctgttccggg gtgagcctga gggcggcgcg gaccgagtgc
      181 tgcttggtag cgtagtcctg ccggaatggc gtgtagccat cgtaggtctt ccagttccag
      241 gccaggtcta gcgccgcggc cgtgccgaac ttgctccgga ccgtggcctg ggagccgagg
      301 gtgtagaggg cctcgtcgga gcggtagatg cgctgctgga aagctcccgc cagggtggcc
      361 gtggtgctct tgccgatctt gatggggctg taggagccct ccaagtccat ggccaagcgc
      421 aggcggtcga tctcgttggc ggtatcgacg aagcggccca ggctcagctt gccgcggctg
      481 gtgaatgatt ggagctccag cccccagtca cggaagtcga tgctggcggt tgcgttgggt
      541 cggcggtcca ggctggagta gctgtcgtcg cccgtgtact ccgtaccatc gaggtcaaac
      601 cggcggtcat agatgacatc gaagtcggcc acgccggtgg gttggcggaa ctccaggttc
      661 gcggtgagct cgcggtcgta gagcccgtcg cccacgtcgt acgacgagta ctgaagcgcg
      721 gcctccgcgt tcgccttctg cgtttcgccc cagagctccc gggccagggt caccgccgcc
      781 gtggtgttgg agcgcgtctg tgtggtcgtc gtcgtgttct gcgtgtagtt cgccgtcaga
      841 cttgttcgtc ccacattacg ggacaggctc aggttggcgg cctgtgtggt gctctgggtg
      901 ctgaccgcgg cacttgattg ccgcaagtca tagctgccgg tcaggttggt gctctggccg
      961 aagcgctgcg aatgggagag gcggcccgtg aagttgcggc tcagggtgcc ataggtgatc
     1021 agggatgagc catagccgct gttcccgagc tcgtatgcgt actcatggct gatcttcgtc
     1081 ccctgcaact ccgtaacgcg gacatcgatg gccccgttgg agttgtagag gtagcgggtc
     1141 tcaccccata ggccctcgta gctgttctgg ccgatggttg ggaagatgtc accccgagtg
     1201 aaccaccggg tacggatcga gaagcccggg aaccagaaga aggtgtgccc cttggccttc
     1261 agcttgatgg cggacgtctc gacgtgcttc ccgcgacggt agacgcgcac gcgacgggcg
     1321 gacgcgccca ggtgtggatc gcggtaccgg tcgcagggcg tcacgaacgc atcatggatg
     1381 accagctcct cgaggtcacc ctcgagccgg tcaccccaga cgtagatggg tgcgctgacc
     1441 tcactgccga agaaactggg cggcaactcc aggcggcact ctttggcccg gtaggtggca
     1501 ttcgtgaaat cgacccacac ccacacggcc tcgatgacct ggtcctgcca ctgcacacga
     1561 acatgcccgg tgaagacggc ctggttcttg ccggtctcga atgtgcagtt gtcggccgtg
     1621 atgacagagt cctggtaagt aatctcgaca ctgccactat agacaatggt atcgcgctct
     1681 ggcacgagtt gtatgtacat ggctttggcg tcgacagcgg acgaggtgct ggccgagggc
     1741 gccgtggcac cggctggcgg ctccaggggc ggtgcggcct gtggcccgat gaagtagtcc
     1801 tcgccggccc cctgcgcgaa cgcaccggcg acggccacac cagcggccgc cgccatcagg
     1861 gtccaccgga ccattcccgc tgtccatcgg cctctcacgc ctggtacgcc cgtccgtcag
     1921 ctgcgccccg cccgcggagc ggtatcaagc ccagtcaccg cctggaagca tcaggcgagc
     1981 cggccgcgcc ggtgggctcc tgtcgcatat acgcatgccc gcacgcgcgc cttcaggggg
     2041 ccgggcgcgc cgccaccacc tggcttccgg gcccggcatg cttcaccctt caggtgcatt
     2101 ggcggtcgct cgggcccggg tggtggtcgg aatggccgta cgccgcccac gtggttactc
     2161 ccatggcgcc gctccgcagc cagccccacg tgccgctgga gccccacgtt accgccaacg
     2221 cccttctttg gggggtctcc gcacacccag catgtcgacg aatgtctcga tacggatccg
     2281 ggtcgcgcca tcgacgggcc ccggccggtc gccctcgagg gtgaggatgg gcaccggcag
     2341 gtgccgtcgt aacagcaggt cggcgatctg gcggaagcag aaactctgaa cataatgaat
     2401 aagtccatcg atacggcgca ccgagatctg agcgccgatg tcatccagtc gaggacctac
     2461 cccgtagggg tacgtg
//
LOCUS       DAOSPL010000666         2475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42295_length_2475_cov_3.552893, whole genome shotgun sequence.
ACCESSION   DAOSPL010000666 DAOSPL010000000
VERSION     DAOSPL010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42295_length_2475_cov_3.552893"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PLQ54_12110"
     CDS             complement(<1..321)
                     /locus_tag="PLQ54_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94052.1"
                     /translation="MRMIQCPDCGIALSLEGHEPGDQIACEGCGRPIEVPGIAYRPPG
                     RRPDTYEEPEDQDQPSEAPDGAGGSAAEPASVEPIAPDGVPAVPTLGVESPPVQEIPS
                     VALVG"
     gene            568..>2475
                     /locus_tag="PLQ54_12115"
     CDS             568..>2475
                     /locus_tag="PLQ54_12115"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOS94053.1"
                     /translation="MDKKTLAKLEFDKIRERLVSCCASSLGRELAERLSPFEREELVR
                     SRMAETTEAVRILERRGRVPFGGVSDFGPALERARVGSALAGEDFVLLRNALQGAAEL
                     RRVLLPEAEACPRVGRLARDMPEFAALLRRIESVVDDDGRVVDGASVELARIRTQCRR
                     LERHIQERMASFLTDPEVQRALQDRLITLRRNRPCLPVRAELKRSVPGVVHDTSASGA
                     TVFIEPAAVVEMGDEYQRLAVAEVEEVARILRELTLCVAERYDELAHLRRLLAILDFI
                     FARGRLSGAMRAEEPQLIAEPQMVLVKARHPLIGPDVVVPIDVAIGEEYDLLLITGPN
                     TGGKTVALKTMGLLAAMALSGLHIPADRGTRIPLLPGLFADIGDEQSIEQSLSTFSSH
                     ISNIAHILRRAPRGSLVLLDEIGVGTDPEEGAALAVAILRHLLRRGMIVAATTHYGAL
                     KTFGLQEPRAQNASVEFDPDTLAPTFRLRTGVPGSSNALAIAKRIGLPREVIADARDI
                     VPRDRVRVEEAILEMERARRDYEARSRALAQATREHERRASMYEEQAKQLAEERKRAL
                     REGWGEAKRVVADAREEARQALAALRKQTREGRATQQVQEELRRLAERVEAAAEAAET
                     PEPTPLPDVRPG"
BASE COUNT          385 a          855 c          841 g          394 t
ORIGIN      
        1 gcccacgagg gccactgagg ggatctcctg cacgggaggc gactcgacgc cgagagtagg
       61 cacggcaggg acaccgtcgg gcgcgattgg ctcgacactc gcgggctcgg ccgctgaccc
      121 gcccgcgcca tctggcgcct cgctgggctg gtcctggtcc tcgggctcct cataggtgtc
      181 cgggcgccgc ccgggtgggc ggtaggctat gcctggcact tctatggggc ggccgcaccc
      241 ctcgcacgcg atctggtcgc cgggctcgtg tccctcgagg ctcagcgcga tgccgcagtc
      301 cggacactgg atcattcgca tggcacgcct cccgaaccgt caccgccgga gcggtccgcc
      361 gagccctcac gccgggcttt caggcgaggt agatccctct aacccgagcc cgcgccgcct
      421 acccgattcg ccgtccggtc ttccggagcc tgcgtgcccc gcgcccagcc cgggatccgc
      481 acgcactcca agaggcttcc acatggcggg ccggaatggg cactcgtatc cggccccagg
      541 gacctcgccg atataatggt tgccgtcatg gacaaaaaga ccctggccaa actggagttc
      601 gacaagatcc gcgagcgcct ggtttcgtgc tgtgcctcga gcctgggccg ggagctggcc
      661 gaacgcctct cgcccttcga gcgcgaggag ctggttcgat cccggatggc cgagacgacc
      721 gaggcagttc ggatcctcga gcgccgcggg cgggtgccct ttggcggcgt gtcggatttc
      781 gggcccgcac tcgagcgcgc tcgagtgggg tccgccctgg cgggcgagga ctttgtcctg
      841 ctccgcaatg ccctgcaggg cgccgccgag ctacgccggg tgttgctgcc ggaggccgag
      901 gcctgcccgc gtgtgggacg gttggcccgg gatatgcccg agttcgccgc actgctccgg
      961 cggatcgagt cggtcgtcga cgatgatggc cgggtcgtgg acggagccag cgtcgaactg
     1021 gcacggatcc gtactcagtg ccggcggctc gaacggcaca tccaggaacg catggcctca
     1081 ttcctcacag atcctgaggt tcagagggcc ttacaggatc ggctcatcac gctccggcgc
     1141 aaccgaccgt gtctgccggt ccgcgccgag ctcaagcgat cggtgcccgg cgtcgttcac
     1201 gacacgtcgg ccagtggggc cactgtgttc atcgaaccgg ccgccgtcgt cgagatggga
     1261 gacgagtacc agcgtctcgc tgtggccgag gtggaggaag tggcacgcat tctgcgcgag
     1321 ctcaccctct gcgtcgccga gcgttacgac gagctggctc acctgcgccg cctcctggcc
     1381 attctggact tcatctttgc ccgaggccgt ctgtcgggcg ccatgcgggc cgaggagccc
     1441 cagctcatcg ccgagcccca gatggttctg gtcaaggcgc gtcacccgct catcgggccc
     1501 gacgtggtcg tgccgatcga tgtggccatt ggcgaggagt atgacctcct gcttattacg
     1561 ggccccaaca caggaggcaa gaccgtggcc ctcaagacca tggggctgct ggccgccatg
     1621 gcgctgagcg gacttcacat tccagccgac cgcgggacac ggatccccct tctgcccggt
     1681 ttgtttgccg acatcggcga tgagcaatcc attgagcaga gcctttccac gttttcatca
     1741 catatcagca acatcgccca catcttgcgc cgcgctccgc gcggatcgct ggtgttgctc
     1801 gatgagatcg gtgtgggcac cgatccagaa gaaggggctg ccctggcggt ggccatcctc
     1861 cgccatctgc tccgccgcgg catgatcgtg gccgcgacca cccactatgg tgcactcaag
     1921 accttcgggc tccaggagcc ccgggcgcag aacgcgtcgg tcgagttcga tcccgataca
     1981 ctggcgccca cctttcgtct gcgcaccggc gttccgggta gcagcaatgc cctggccatc
     2041 gcgaagcgga tcggcctccc cagggaggtc atcgccgatg cacgcgacat cgtgccccgc
     2101 gatcgcgtgc gggtcgagga agcgatcctc gagatggagc gggcgcggcg cgactacgag
     2161 gcgcgctccc gtgcgctcgc ccaggccacc cgggagcacg aacgccgggc ctccatgtac
     2221 gaggagcagg cgaagcagct tgcggaagag cgaaagagag ccctccgaga gggatggggt
     2281 gaggccaaac gcgtggtcgc cgatgcccgc gaggaagccc gccaggctct ggccgcgctc
     2341 aggaagcaga cccgagaggg ccgggccacc cagcaggtcc aggaagagct tcgccgcctg
     2401 gccgagcgag tcgaagcagc ggctgaggcg gcagagactc ccgagccgac gccgctgccg
     2461 gacgtgcgcc cgggc
//
LOCUS       DAOSPL010000667         2474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42337_length_2474_cov_2.748656, whole genome shotgun sequence.
ACCESSION   DAOSPL010000667 DAOSPL010000000
VERSION     DAOSPL010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42337_length_2474_cov_2.748656"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PLQ54_12120"
     CDS             complement(<1..300)
                     /locus_tag="PLQ54_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOS94054.1"
                     /translation="MLGGALSWRRADGLDFAKALPGTTPWGTSQFVGADLRWGLEAAW
                     DYRRLRLRSEWIQARLSPAGQQPLGLSAGGGYVQAAYELSRRLEVAAKLERFDPDR"
     gene            complement(300..812)
                     /locus_tag="PLQ54_12125"
     CDS             complement(300..812)
                     /locus_tag="PLQ54_12125"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOS94055.1"
                     /translation="MTSYFQARVTADDGPAVHLGLRRLKLTVEGGIGRDASYLVQGLY
                     KTGNNSATDGRVYLQEALVRLRAGDGTITVGQFKPPFGMERFTPDWDLASIDRSPPTP
                     SSPTANSARARVSPATAASSGTRDLITAGSSLPLVCSRDTGPTRPCDRPPHSSLDGWY
                     GRLTGASGRR"
     gene            complement(925..1686)
                     /locus_tag="PLQ54_12130"
     CDS             complement(925..1686)
                     /locus_tag="PLQ54_12130"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOS94056.1"
                     /translation="MTMTDLREARDELVRILELTSEPVGVRFIPQGQALPEDCQTPAS
                     RRYCQALMEARRGARVLLTPDNVSCPAAAAAFGFRPLPPMLASGEMLHSFGIFGSAEA
                     GKATIDSMRRLRPGEYSAVALGPLAALHGEPSVVVVEGTVEQVMWIALAARHQTGGRT
                     EFSTAILQATCEDATLLPFLTERLNATLGCYGCRDATDMGVDETAVGFPYSMLPAVVD
                     GLRALGNKALPSARSKTAYRALSKGAACDTGMAAQ"
     gene            1836..2024
                     /locus_tag="PLQ54_12135"
     CDS             1836..2024
                     /locus_tag="PLQ54_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94057.1"
                     /translation="MASMVQFVRASLATTQWQLSAYIPLVMVQAWRSWTSFTLARAVL
                     RSLATARPAVHPAWLSLQ"
     gene            complement(2010..>2474)
                     /locus_tag="PLQ54_12140"
     CDS             complement(2010..>2474)
                     /locus_tag="PLQ54_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOS94058.1"
                     /translation="PLLKWLFVGFLVFAASMMLFYKAKQRDAAPSTRRLVGYGLGTGT
                     VAGYLGGLLGVGGGNFIVPVLVWLGFDPKKASATTAFVVIFSSVSGFLGHVSLGAMDM
                     ALLAATCAGSIGGALLGAWLMQKKLQSGQVKVAIGVILYAIAAQMVWKLLQR"
BASE COUNT          417 a          858 c          823 g          376 t
ORIGIN      
        1 cctgtcggga tcgaaccgtt cgagcttggc agcgacttcg agtcgacgcg agagctcgta
       61 ggccgcctgg acgtagcctc caccggccga tagcccaaga ggctgctggc ctgccgggct
      121 caagcgggcc tggatccact cgctgcgcag cctgagccgg cgatagtccc atgctgcctc
      181 gagaccccac cgcaggtcgg cgcccacaaa ctggctggta ccccagggcg tggtgccggg
      241 cagtgccttg gcgaagtcca ggccgtccgc ccggcgccaa ctcaaggcac ccccgagcat
      301 cagcgtcgac cgctggcccc ggtgagccgt ccataccacc cgtccagcga cgagtggggc
      361 gggcggtcgc acgggcgtgt tggccccgtg tccctcgaac agaccaacgg cagtgaagag
      421 cctgccgtga tgagatcgcg cgtcccactg gaggccgcgg tcgcgggtga aaccctggcc
      481 ctggccgagt ttgccgttgg ggacgaggga gtcggtggcg atcgatcgat cgatgccagg
      541 tcccagtccg gcgtgaaccg ctccatgccg aagggtggct tgaactggcc gacggtgatg
      601 gtcccgtcgc cggcgcgcag gcgcacaagc gcctcctgca ggtagacacg cccatcggtg
      661 gcgctgttgt tgccggtctt gtagaggccc tgaaccaggt agctggcatc ccggccgatc
      721 ccgccctcga cggtgagttt gagtcgcctg aggcccaggt gaacggcagg tccgtcgtcc
      781 gcggtgacgc gggcctggaa gtaggaggtc acctcggtgt ccgccgcgca taccgagcag
      841 acgagtgccg ccgccagggc gagtgcagcc caagatgctc gtctgggccc ggcggcgggg
      901 cgggttccag gccgcccgcc ccccctactg ggcagccatg cccgtgtcac aggctgctcc
      961 cttcgatagc gcccggtacg ccgtcttcga acgagccgac ggcagcgcct tgttccccag
     1021 agcccgcaat ccgtctacga cagcaggcag cattgagtaa gggaacccga cggcagtctc
     1081 gtcaaccccc atgtcggtcg cgtcgcggca gccatagcaa ccgagggtgg cgttgagccg
     1141 ctcagtcagg aacgggagca gcgtcgcgtc ctcgcacgtc gcttgaagga tcgcggtgga
     1201 gaactcggtc cggccgcccg tctggtgccg ggccgccagg gcgatccaca tgacctgctc
     1261 taccgtgccc tcaaccacca cgacactggg ctcaccgtgc agggctgcca gcggcccgag
     1321 agcgacggcc gagtattcgc cggggcgaag acgtctcatg gaatcgatcg tcgccttgcc
     1381 ggcttcggcg gagccgaaga tgccgaagga gtggagcatc tcgcccgacg ccagcatcgg
     1441 cgggagtggt ctgaacccga aggccgcagc ggcagcggga catgacacgt tgtcgggtgt
     1501 gagaagcacg cgcgcccccc gccgcgcctc catcagggcc tggcagtagc ggcggctcgc
     1561 cggcgtctgg cagtcctccg gcagggcctg gccctggggg atgaaccgca ctccgactgg
     1621 ctcggaagtc agctccagga ttcggacaag ctcgtcacga gcctctctca ggtcagtcat
     1681 cgtcatcgcg cctaaccttc tccttcccgg cgccagactc cgggcctgac cgcaggtctc
     1741 atcatgcgtg ttcgccggta caccatgcgg ctcggcgagt gtccaactga atagtgtgat
     1801 gcgctagccc gaaccgctcg tgccgcaggc cccgaatggc ctccatggtg cagttcgtca
     1861 gagcgtcgct ggccactacg cagtggcagc tcagcgcgta catcccgctg gtgatggtcc
     1921 aggcgtggag atcgtggacg tccttcacgc tcgcacgcgc cgtgctccga tcgctcgcca
     1981 cggccaggcc agccgtccac ccggcgtggc tatcgctgca gtagcttcca gaccatctgt
     2041 gcggcgatgg cgtacaggat gacgccgatg gccaccttga cctgaccgct ctgcagcttc
     2101 ttctgcatca gccacgcgcc gagcagggcc ccaccgatcg agccggcgca ggtggctgcc
     2161 agcaacgcca tgtccatcgc cccgagggaa acgtgcccga ggaacccgga caccgaggag
     2221 aagatgacga cgaaggcggt ggtggccgaa gctttcttgg ggtcgaagcc caaccagacg
     2281 aggacgggaa cgatgaagtt cccgcccccc acacccagca gcccgcccaa gtagccggcg
     2341 accgtccccg tgcccagccc ataccccacg agcctccgtg tggagggcgc ggcatcgcgc
     2401 tgcttcgcct tgtagaacag catcatcgac gcggcgaaga cgaggaagcc cacgaagagc
     2461 catttgagca gcgg
//
LOCUS       DAOSPL010000668         2472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42370_length_2472_cov_3.499793, whole genome shotgun sequence.
ACCESSION   DAOSPL010000668 DAOSPL010000000
VERSION     DAOSPL010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42370_length_2472_cov_3.499793"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PLQ54_12145"
     CDS             complement(<1..348)
                     /locus_tag="PLQ54_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOS94059.1"
                     /translation="MTPGSPGHEVRPGFPIASVGLLVSPHKERALPCAQRLAADLRDR
                     GVAVRVGPELAAAIEGSDVGDHAFVHGADLLIVAGGDGTILGAARRAAPVGTPILGVY
                     VSGFGFLTETDSDQ"
     gene            complement(345..1799)
                     /locus_tag="PLQ54_12150"
     CDS             complement(345..1799)
                     /locus_tag="PLQ54_12150"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS94060.1"
                     /translation="MVAARFLWICAAALAVATALDADEPSLEAGKAAFEAGDYVTAMG
                     VFEALIGADPGNAEALSWLGCAQLRAGRPQDAVVSLREAAHLAPRDAVIRSNLGNALA
                     ATGDTDGAIASYRAALVADPTLDDARYNLALTLVEAGQIDAATTEMGPLLNDPAKSGP
                     AHQRLAGLLVSAGKHRAAASHYQAARSSPELAGEATVGLARCLIAMGETERAIQTLEE
                     ETGHGRATPPVRELLGVLYESTGQPAKCIEVLQSLRAEGQDTFELHRALGSAQAQAGD
                     YAEAAASFQRALAHRPRDAETENNLGWAYAQQLADDKAEQHLVAALEIEPALVEARRT
                     LVMVYRRQGRTAEVVAQLEAILAAQTGDDQDLRALIRASLSSPDRKLSDTTVKRVTEI
                     QTTDPVLLGAAGALLIQQARYSEAVQLLRRALERSNSDASLHNNLGVALERLGQLKDA
                     MVEYRTAHHLRPDMVEAERNRQRVEDMLAREAAP"
     gene            1909..>2472
                     /locus_tag="PLQ54_12155"
     CDS             1909..>2472
                     /locus_tag="PLQ54_12155"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOS94061.1"
                     /translation="MDELATARRVALLAGEAILRQYARFTPVPDAPATITTDADRQAQ
                     DLILELIAEEFPDDAFLAEEPTEALGQLRREGRRLWVIDPIDGTRGFARKTDDFCTMV
                     ALAVDGVPVVGVVHEPVPGRTTWAVRGGGCLCKMVSEEAPRRVRVSSTAVAAQATVVV
                     SRGRQGRPPSPQEQYGVRNALFSFSCGT"
BASE COUNT          357 a          857 c          870 g          388 t
ORIGIN      
        1 ctggtcgctg tcggtctcgg tgaggaaccc gaagcccgag acgtacacgc cgaggatcgg
       61 ggtcccgacc ggggccgcgc gccgcgcggc gcccaagatg gtgccatcgc cccccgcgac
      121 gatgagcagg tcggccccat gcacgaacgc atggtcgccc acgtcggagc cctcgatggc
      181 ggcggccagc tcagggccca cccggaccgc cacccctcgg tcccgcagat cggcggccag
      241 ccgctgagcg cacggcaggg cgcgttcctt gtgcggcgac acgagcagcc cgaccgaggc
      301 gatcgggaac ccgggccgca cttcgtgtcc tgggctgccc ggcgtcatgg tgcggcctcc
      361 cgggccagca tgtcctcaac ccgctggcgg tttcgttccg cttccaccat gtctggcctc
      421 aggtggtgcg cggtccggta ctcgaccatg gcgtccttga gctgccccag acgttcgagc
      481 gccacgccca ggttattgtg gagcgacgcg tcggagttgc tcctctcgag ggctcgccga
      541 agaagctgca cggcctcgct gtaacgcgcc tgctggatca gaagagcacc cgcggcgccc
      601 agcagaacgg ggtcggtggt ctggatctcc gtcacccgct tcacggtcgt gtccgagagc
      661 ttgcgatccg ggctgctcag gctggcgcga atgagcgcgc gcagatcctg gtcgtccccg
      721 gtctgtgcgg ccaggatagc ctcgagttgg gccacgacct cagcagtccg gccctgccgc
      781 cggtagacca tcacgagggt gcggcgggcc tccacgagcg ccggctcgat ctccagcgcg
      841 gccaccaggt gctgctcagc cttgtcatcc gcaagctgtt gggcatacgc ccagcctagg
      901 ttgttctccg tctcggcatc gcgtgggcga tgcgcgaggg cacgctggaa cgacgcggca
      961 gcctcggcat agtctccggc ctgcgcttga gccgatccga gcgcgcggtg gagctcaaag
     1021 gtatcctggc cttcagcacg aagcgactgg agcacctcga tgcacttggc gggctgaccg
     1081 gtactctcgt acagcacgcc aaggagttcg cgcaccggcg gagtagcccg accgtgtccg
     1141 gtctcctcct cgagggtctg gatggcgcgc tccgtctccc ccatggcgat gaggcaccga
     1201 gccagcccca ccgtcgcttc gcccgcgagc tcgggcgaag agcgagccgc ctggtaatgc
     1261 gaggccgcgg ctcggtgctt gccggcagac acgagcagtc cggcgagcct ctggtgggcc
     1321 ggaccgctct tggcgggatc gttcaggagg ggacccatct cggtcgtggc cgcatcgatc
     1381 tgcccggctt cgaccagagt gagggccagg ttgtagcgag cgtcatcgag tgtggggtcg
     1441 gcgaccagag cggcgcggta gctggcgatc gccccatcgg tgtcgcccgt ggccgccaac
     1501 gcgtttccca ggttgctgcg gatcacggca tcgcgagggg ccaagtgggc ggcctcgcgg
     1561 agtgagacca cggcgtcttg agggcgtccg gcccggagct gcgcacaccc caaccagctc
     1621 aatgcctcgg cgttccccgg atcggccccg atcagtgcct cgaacacacc catggccgtc
     1681 acatagtccc ccgcctcgaa cgcggccttg cccgcttcga gactgggctc gtccgcatcc
     1741 agggcggtgg ccacggccag cgccgcggca cagatccaca ggaacctcgc tgcgaccaca
     1801 gcccgttccc ccttccgagg tatacttcgc cgaggtgcag gctcaggcct tggtggtctg
     1861 accggcctga gggccttcgg cggggggggc gtcagcacga tggactggat ggacgaactg
     1921 gcgaccgccc ggcgcgtcgc gctgctggcg ggggaggcga ttttgcgcca gtatgcccgt
     1981 ttcacgccgg ttcccgatgc gccggccact atcacaaccg acgccgaccg ccaggcgcag
     2041 gacctgatcc tcgaactcat cgccgaggag ttccccgacg acgcgtttct ggccgaggag
     2101 cccacggagg ccctggggca gctccggcgc gaaggccgtc ggctgtgggt gatcgacccg
     2161 atcgacggaa cgcgcggttt cgcacgcaag actgacgact tctgcaccat ggtggcattg
     2221 gccgtggacg gggtgcccgt ggtcggcgtg gttcacgagc ccgttcccgg gcgcacgacc
     2281 tgggcagtgc gcggcggggg ctgcctctgc aagatggtca gcgaggaggc cccacggcgg
     2341 gttcgcgtgt cgtccacggc ggtggcggcg caggccacgg tggtggtgag ccgtgggaga
     2401 caggggcgac cgccgtcgcc ccaggagcag tacggcgtac ggaacgcatt gttcagcttc
     2461 tcctgcggca cg
//
LOCUS       DAOSPL010000669         2471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42395_length_2471_cov_3.240894, whole genome shotgun sequence.
ACCESSION   DAOSPL010000669 DAOSPL010000000
VERSION     DAOSPL010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42395_length_2471_cov_3.240894"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1453
                     /locus_tag="PLQ54_12160"
     CDS             <1..1453
                     /locus_tag="PLQ54_12160"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HOS94062.1"
                     /translation="VRLLALAGVVALAGCGLGGDSLTESESGSVGRVVVRVKWPEAPS
                     STRVIPDAARSISLYIIYADATRTAGNIDYPETEKVFDPVRAGAATLQATAYASAAHR
                     DPWLARGEANVSIKPNIDGITYASVILSEPETDRRAYDVADVRWGYYGTGAGEFQAPC
                     KLAADGDLTVYVLDTQQSKIAQYDLSSGDPVLSREIGGGQGVSLGQLSYPYDIAVHPE
                     THDIWVADTGNNRIQVFSRGGEYQAGCPSSPWDPMVFISPRSIDIDPFGTVFVLDGPA
                     VDSSTGLAAYRIVQVNAKFRDISRQLIPVGSGEGQLKDPQGMAVDRYGNCFVADTGNK
                     RIVKFGRAGSLRTQWTYTFACGSVVDEMLANDVDVDDAGNVCVIGSTRDRSLFKFDNS
                     GNLVAYHNVSMKQMEALFGHMSNVKLATGIAVTPLGRVYVFDGYPVDLSNPTSGLFGN
                     GAVMRLDPEASDFVTCLPASRACTIRVVPSGDE"
     gene            1504..>2471
                     /locus_tag="PLQ54_12165"
     CDS             1504..>2471
                     /locus_tag="PLQ54_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94063.1"
                     /translation="MSRGIAWLLIAVMVGIAGCGGGPAPVDGNNDNHGNGGNNGGDPT
                     ATHFADDPNAPEVVTVTGYVDRTAIGGEGLTVLSAYQQSAALSATGSFRTQVSGEGPQ
                     LLFALDQNRTLRGLAVTVPATAGASSRAAPQFDADSTAMGLLFLTPGILDTDPEGAAK
                     VIAELKGYGTFSTLSRYLATKLPTEALADLVANDEHLSDLLSDCYSQWCDYHAPAAPE
                     ALARDINDYDVKLRVEGPSPWSRFKVPGAGTGGTTWNPGKPRDVDVFMENHGMRWVNI
                     HRHPVDTDGEYLDWETVADGINSMPGAVPLSIGSLLTWTQFQEAKGL"
BASE COUNT          438 a          760 c          856 g          417 t
ORIGIN      
        1 cgttcgcctc ctggccttgg cgggcgtggt ggccctcgcg gggtgcggac tcggcggcga
       61 ctcgcttacc gagtcggaga gcggctcggt ggggcgggtc gtggttcgcg tgaagtggcc
      121 cgaggctccc tcgagcactc gcgtcatccc ggatgccgcg cggtcgatca gcctctacat
      181 catctacgcc gatgccactc ggaccgcggg gaatatcgac tatccggaga ccgagaaggt
      241 tttcgacccg gttcgtgcgg gcgccgcgac gttgcaggcg acggcatacg ccagcgccgc
      301 acacagagac ccgtggcttg cccggggcga ggccaacgtg tcgatcaagc ccaacatcga
      361 tgggatcacc tacgcgtcag tgatcctctc tgagccggag accgaccggc gggcgtacga
      421 cgtggccgat gtccgctggg gctactacgg cacgggtgcc ggtgagttcc aggcaccgtg
      481 caagctcgcc gccgatggcg acctgaccgt gtacgtcctc gacacgcagc agagcaagat
      541 agcccagtac gacctgtcca gcggcgatcc ggtgcttagt cgcgagatcg gtggggggca
      601 aggtgtgagc ttgggtcagc tctcgtatcc ctacgacatc gcggtgcacc ccgagacgca
      661 cgacatatgg gtcgccgaca caggcaacaa ccgcattcag gtgttctcga gaggcggcga
      721 gtaccaggcg ggttgtccga gctcgccgtg ggacccgatg gtgttcatat cgcctcggtc
      781 catcgacatc gacccgttcg gcaccgtctt cgtgctggac gggccggcag tggactcgtc
      841 gaccgggttg gccgcctacc gcatcgtgca agtgaacgcc aagttccgtg acatctcgcg
      901 ccagctcatt ccggtgggct cgggcgaggg tcagctcaag gacccacagg gcatggcggt
      961 ggaccgctat gggaactgtt tcgtggccga cacgggcaac aagcggatcg tgaagttcgg
     1021 tcgagcgggc agtctccgca cgcagtggac ttacacattc gcctgtggga gcgtcgtcga
     1081 cgagatgctg gccaacgatg tcgatgtcga cgacgcgggc aacgtctgcg tcatcggctc
     1141 gacccgggat cgcagtctgt tcaagttcga caactctggc aacctggtcg catatcacaa
     1201 cgtgagcatg aagcagatgg aagcgctgtt cgggcacatg tcgaacgtga agctggcgac
     1261 ggggattgcg gtgacgccac tgggtcgtgt ctatgtcttc gatgggtacc cagtggatct
     1321 ctcaaacccg acgtccgggt tgttcggtaa cggagcggtc atgcgcttgg atccagaggc
     1381 gagcgacttc gtgacctgtt tgccggcaag cagggcgtgc acgatacgag tcgtcccgtc
     1441 gggcgacgag tgagggaaca tgagaggagc gcgacgctgg tgacccgacg cgtccgggtg
     1501 ggcatgagca ggggtatagc gtggctgctg atcgcggtca tggtggggat tgcgggctgc
     1561 gggggagggc cagcgccggt cgacggcaac aacgacaacc acggcaacgg tggcaacaac
     1621 gggggcgacc ccaccgccac ccacttcgcg gatgacccga acgcgcccga ggtggtcacg
     1681 gtcaccggtt atgtggaccg cacggccatc ggcggcgagg ggctgacggt gctctcggcg
     1741 taccaacaga gcgccgcact ctccgcgacc ggcagtttcc ggactcaggt gtctggcgaa
     1801 ggtccgcagc tcctgttcgc gctggaccag aaccgcacac ttcgtggcct ggccgtgacg
     1861 gttcccgcga cggcaggggc gtcatcgcgc gcggcgccgc agttcgacgc cgatagcacg
     1921 gccatgggat tgctgttcct gacgccgggc attctcgaca cggacccaga gggtgcggcg
     1981 aaggtcatcg ctgagctcaa agggtacggc accttcagca ctctgtcccg ctacctggcg
     2041 acgaagctcc cgacagaggc tctggctgac ctggtcgcca acgatgagca cctatcagac
     2101 ctgctgtcgg actgctactc gcagtggtgc gactaccacg ctcccgcggc tcccgaagct
     2161 ctggcgcggg acatcaacga ctatgacgtg aagctcagag tggaaggtcc gagcccttgg
     2221 tcgcgcttca aagtgcctgg agcgggcacg ggcggcacca cgtggaaccc gggcaagccg
     2281 cgcgacgtgg acgtcttcat ggagaaccac ggcatgcgtt gggtgaacat ccaccggcac
     2341 ccggtcgaca ccgacggcga gtacctggac tgggagacgg tggcggacgg catcaactcg
     2401 atgcccggcg cggtgccact gagtattggc tcgctgctga cctggacgca gttccaggag
     2461 gcgaaggggc t
//
LOCUS       DAOSPL010000670         2464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42555_length_2464_cov_3.545870, whole genome shotgun sequence.
ACCESSION   DAOSPL010000670 DAOSPL010000000
VERSION     DAOSPL010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42555_length_2464_cov_3.545870"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1716)
                     /locus_tag="PLQ54_12170"
     CDS             complement(<1..1716)
                     /locus_tag="PLQ54_12170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOS94064.1"
                     /translation="MALVDLLARDDVPEHVKQAIREELDAARESEERYRLLAANATDM
                     IIRLAPDGTIIDVSPSSRHLSGYDPAELIGRCGRDFVYPNDLGTLLRAQEALLDRPEA
                     QTIEWRLIHKNGAPVWIETTTQAVRGPEGTITEFVAVGRDISDRKRAEDELAKRLDLE
                     SRVSHMLGSLIQLPPGELDNGIREALEMLSTSVGAQRAGVFVIDRAAGTAARTHGWSA
                     HPEDAWDRGTLAIDTELFAHSIRLMERGEILRVSRPTDLPRSAVGEQALWDADGFRAG
                     LFFPLGSGETLYGALGFYGPTGREFAWPDHFISLVTVVADALLALLRRREAEEALIER
                     EARLQSVFRSAPVGIGLTRDRVILEANDRLCEMTGYAREELLGQSARMLYGSDEAYEV
                     VGREKYRQIREQGTGTVETCWRRKDGTFIDVLLSSTPLHGSEGSGEITFTALDITARK
                     SAEAELRRTQGLLQAAIDQSSSGIVIADAPDVRVRMVNAAAANLRGREPEPVSGDPGE
                     RATWQWHARRPDGTAIAFGDLPLARAIQRGEVVRNQLLIVQGSDGGERWISANAAPVR
                     DADGTV"
     gene            1911..>2464
                     /locus_tag="PLQ54_12175"
     CDS             1911..>2464
                     /locus_tag="PLQ54_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94065.1"
                     /translation="MGEIHLRTSRDALTVDEATGAITKFRPLGAPGTELQSECGRCAF
                     MLHHLDGDGVYRWTDSTKARSVVASVDRAGSESLVRLVYQGVGPLDLEVCLTIRASEA
                     WPVTRWSASVRAGHGVRVVDLQMPVIVVPWSEVSSVVEPYAFGRLIEGAGLESLPEDS
                     PGLWQLTPENGDTAHYPGSVVAQFL"
BASE COUNT          371 a          796 c          877 g          420 t
ORIGIN      
        1 gacggtgccg tcggcgtcgc ggaccggtgc cgcgttggcc gagatccatc gctcgccacc
       61 atccgagccc tggacgatca ggagctggtt tcgcaccacc tcaccacgct ggatggcgcg
      121 ggccaggggg aggtccccga aggcaatggc cgtgccatcg gggcgtctgg catgccactg
      181 ccatgtcgcc cgctcgcccg ggtcgcccga gacgggttcg ggctctctac cgcgcaagtt
      241 ggcggctgcg gcgttgacca tgcgaacgcg cacgtccggc gcatcggcga tgacgatccc
      301 ggacgaggac tgatcgatcg ccgcctgaag cagcccctga gttctgcgga gctcggcctc
      361 agcgctcttg cgggccgtga tgtcgagtgc cgtaaaggtg atctctccag atccctccga
      421 gccatggagt ggtgtggagc tgagcagaac atcgatgaac gtgccgtcct tacgccgcca
      481 gcacgtctcg acggttccgg taccctgctc gcggatctgg cggtatttct ctcggccgac
      541 cacctcatag gcctcgtcac tgccgtagag cattcgggcg ctctggccga gcagttcctc
      601 tcgggcgtag ccggtcatct cgcacagccg gtcattggcc tcgagtatga cgcggtcgcg
      661 agtgagcccg atgcctaccg gggcggagcg gaagacgctc tgcaaccgcg cctcgcgctc
      721 gatgagggcc tcctcggctt ctcgccggcg gagcagtgcg aggagggcat cggccacgac
      781 ggtgacaagg gagatgaagt ggtcgggcca tgcgaactct cgcccggtcg gcccgtagaa
      841 gcccagagcg ccgtagaggg tctccccact gcccaacggg aagaacaggc ccgcccggaa
      901 cccgtccgca tcccacaggg cttgctcccc cacggcggag cggggaaggt cggtggggcg
      961 ggacacgcgc aggatctcac cccgctccat caggcggatc gagtgggcga acaactcggt
     1021 atcgatggcc agtgtgcctc gatcccaggc atcttcggga tgagccgacc atccgtgcgt
     1081 ccgcgcggcg gtcccggcgg cgcggtcgat gacgaacacg cccgcccgct gggcccccac
     1141 cgacgtggag agcatttcca gggcctcgcg gatgccgttg tcgagctcac cggggggcag
     1201 ttgtatgaga gagcccaaca tgtgcgagac gcgcgactcg aggtccaggc gcttggcgag
     1261 ctcgtcctca gctctcttgc ggtcgctaat gtcgcggccg accgccacga actcggtgat
     1321 ggtaccctcg gggccacgca cggcttgggt ggtcgtctcg atccacaccg gggccccgtt
     1381 cttgtggatc aaacgccact cgatcgtctg tgcctccgga cggtcgagga gggcctcttg
     1441 agcgcggagg agtgtcccca agtcgttggg gtacacgaag tcgcggccgc accggccgat
     1501 gagctcggcg gggtcgtagc ccgatagatg gcggctggac ggcgaaacgt cgatgatggt
     1561 gccgtccggc gccaggcgga tgatcatgtc ggtggcgttg gcggcgagga gccgatagcg
     1621 ctcctcgctc tcgcgggcgg cgtcgagctc ctcgcgaatg gcctgcttca cgtgctcggg
     1681 cacatcatcg cgggccagta gatctactag ggccaacggc gaacacctca acaatgtcga
     1741 ccggcggcgg ggcagttcgg cccgtgcttt cggccggtca gtgccgtaca cctgtgctgc
     1801 atccgtatgc aagatccagc gtggagggac cggccgaggt tccccgtggg aggatcccgg
     1861 ttcgtcgagc gggaaccggc cggcagacca ctacgtcgag gtgtgttcgc atgggcgaga
     1921 tccacctgcg cacttccaga gacgcactca cagtcgatga ggccaccggg gccattacga
     1981 agttccggcc cctgggggcg ccaggcacgg agctgcagtc tgagtgtggt cgctgtgcgt
     2041 tcatgctgca ccacctggac ggcgacgggg tgtaccgctg gacggactcg acgaaggccc
     2101 ggagtgtcgt ggcctccgtg gatcgggccg gctccgagtc gctagtgcgc ctggtgtacc
     2161 agggcgtggg gcctctcgac ctggaggtgt gcctcaccat ccgggcgtct gaggcctggc
     2221 cggttactcg ctggtcggcg tcggtgcggg cgggtcacgg cgtgcgggtc gtcgacttgc
     2281 agatgcccgt gatcgtggtg ccgtggtctg aggtgtcttc ggtcgtggag ccgtatgcct
     2341 tcggccggct gatcgaggga gcggggctcg agagtctgcc ggaggactcg cccggtctgt
     2401 ggcagctcac gcccgagaac ggcgacacgg cgcactaccc ggggagcgtg gtcgcccagt
     2461 tcct
//
LOCUS       DAOSPL010000671         2464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42562_length_2464_cov_2.942300, whole genome shotgun sequence.
ACCESSION   DAOSPL010000671 DAOSPL010000000
VERSION     DAOSPL010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42562_length_2464_cov_2.942300"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="PLQ54_12180"
     CDS             <1..1300
                     /locus_tag="PLQ54_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HOS94066.1"
                     /translation="ALGSNHEPGLAADITFVDLATSQTTQLRLPTGCNEVEQLVCSPD
                     GEWVYVVSLLSRFLVPTTQIQRGWINTNALSVIHVKTRQLLATVLLDDLDLGAANPYG
                     VAMTKDGTRLFVTISGSHEVMIVDVPKLHEIIEATPAEERPELANDLTFLYRNDAKKR
                     IKAEGFGPRGLTLTADERGLLVANYFSDTVSLIDARSGKLVRTIALGPEPKMDGVRRG
                     EFLFADATMCFQHWQSCLSCHPDGRSDGLMWDLMNDGLGNPKNAKSLLLSGATPPVMA
                     HGVRDTMRTAIDAGFRYILFRVPEEADVDAVEIYLNSMKPSRSPVLDNKEMKKRADRG
                     RKLFMDEKRTGCVTCHPAELYTDLQMHNVGTQSQFDREGEFDTPTLVELFRSAPYLHD
                     GTAVTLKDVLTTRNTGDMHGKTSGLSPEEIEDLALFLQSL"
     gene            1378..>2464
                     /locus_tag="PLQ54_12185"
     CDS             1378..>2464
                     /locus_tag="PLQ54_12185"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOS94067.1"
                     /translation="MTGSPNCASEACASVTIAREGGLTVARLDRPRLRELYLSAAPAA
                     PSTAAGEAEWAYEALTCALHEAGAVGVQERVFGSLECADEILATRERVLSPWFARTTR
                     WPAAFIEGSPPGRTGLSGVHLYAVAGPECTPIRVGERLCGMQFGHWGMTQVYVTSPAV
                     GGPDCRPGDAAQRMIRNAQSYLHSQGAGYRDVVRTWIYLRDILDWYSAFNEVRSGLYA
                     EWGLADPGSQEFVPASTGIAGRPRDGHWGSMDVLALVGPGRAHAQIRPLHNPLQNEAY
                     SYGSAFSRAMSVDYGGLDVVYVSGTAAIDEQGRSVNVGDLEGQVVKTLENVDALIGTR
                     GMSLDDIVTSTVFIKPGQDANVVRRVIA"
BASE COUNT          422 a          816 c          812 g          414 t
ORIGIN      
        1 tgcgctgggg tcaaaccacg agcccggcct ggccgccgac atcaccttcg tcgatctggc
       61 cacgagccag actactcagc tccggctccc gaccgggtgc aatgaggtgg agcagctcgt
      121 ctgctcgccc gatggcgagt gggtctacgt ggtgagtcta ctgagccggt tcctcgtgcc
      181 gacgacccag atccagcggg ggtggatcaa caccaacgcg ctgagtgtga tccatgtcaa
      241 gaccaggcag ttgctcgcca cggtgctcct ggacgatctc gacctgggcg cggccaatcc
      301 ctacggcgtg gcaatgacca aagacggcac caggctgttc gtgaccatat cgggcagcca
      361 cgaggtgatg atcgtcgatg tgcccaagct acacgagatc atcgaggcca ctccagcaga
      421 ggagcggccc gagctggcca acgatctgac gttcctctac cgcaacgatg cgaagaagcg
      481 catcaaggcc gaggggttcg ggccgcgcgg gctcaccctc acggcggacg agcgcggctt
      541 gctggttgcc aactacttct ccgataccgt tagtctcatc gatgcgcggt cggggaagct
      601 cgtgcgcacc atcgccctcg ggcccgagcc gaagatggat ggcgtccgcc gtggggagtt
      661 cctgttcgcc gatgccacca tgtgcttcca gcactggcag tcttgcctga gctgccatcc
      721 ggacggccgg tccgacggcc tcatgtggga cctgatgaac gatggcctgg gcaaccccaa
      781 gaacgccaag tcgttgctgc tgtcgggcgc cacgcctccg gtgatggcgc acggcgtccg
      841 cgacaccatg cgaaccgcca tcgatgccgg cttcaggtac atcctgttcc gagtgccgga
      901 ggaggcggat gtcgatgcgg tcgagatcta cctgaacagc atgaagccct cgcgcagccc
      961 agtgctcgac aacaaggaga tgaagaaacg cgccgaccgg ggccggaagc tgttcatgga
     1021 tgagaagcgc accgggtgcg tcacgtgcca cccggccgag ctctacactg accttcagat
     1081 gcataacgtc ggcactcaga gccaattcga ccgcgagggc gagttcgaca cgcccacgct
     1141 agtcgagctg ttccgctcag ccccgtacct gcacgacggt acggccgtca cgctcaaaga
     1201 cgtgctgacg acacggaaca ccggcgacat gcacgggaag acgtcgggtc ttagccccga
     1261 ggagatcgag gacctcgccc tcttcctgca gtcactgtga tccccgatcc ccgctgccct
     1321 ttggcgccga ccacggggtg catgcccgtg gtatccggag agtgacgtgg actgaccatg
     1381 acgggttcgc ccaactgcgc ctctgaggct tgtgcctccg tcaccatcgc ccgcgagggg
     1441 ggtctgaccg tcgctcggct cgaccgcccc aggctgcggg agctgtacct gagtgcggcg
     1501 cctgcggcgc cgtcgacagc agccggggag gccgaatggg cgtacgaagc ccttacctgc
     1561 gccctgcacg aagctggggc cgtcggggtc caggagcgtg tgttcggaag cctcgaatgt
     1621 gctgatgaga tcctcgccac ccgtgagcgt gtcctctcgc cttggttcgc ccggaccacc
     1681 cggtggcccg ccgccttcat cgagggcagc ccgcccggtc gcaccgggct gtcgggtgtg
     1741 cacctctatg ccgtcgcggg gcccgagtgc acgccgatcc gtgtcggcga gcggctctgc
     1801 gggatgcagt tcgggcactg gggcatgact caagtctacg tcaccagccc ggccgtgggc
     1861 ggccccgact gtcgtcccgg tgatgccgcc caacggatga tccggaacgc ccagtcgtac
     1921 ctgcacagcc aaggggcggg ctaccgcgat gtggtgcgga cgtggatcta cctccgcgat
     1981 atcctcgact ggtatagcgc cttcaacgag gtgcgttcgg ggctctacgc ggagtgggga
     2041 ttagctgatc ccggctcgca ggagttcgtc cccgccagca cgggcatcgc cggccggccg
     2101 cgcgatggcc actggggctc catggacgtc ctggcacttg tgggcccggg ccgcgcccac
     2161 gcccagatcc gcccgctgca caacccgctg cagaatgaag cctatagcta cgggtcggcc
     2221 ttctctcggg ccatgtcagt ggactacggc gggctcgatg tggtctacgt gtcgggcacg
     2281 gcggccatcg atgagcaggg gcgctcagtg aacgtcggcg accttgaggg ccaagtggtg
     2341 aagacgctcg agaacgtcga tgccctgatc ggtacccggg ggatgagcct ggacgacatc
     2401 gtaacctcga cggtattcat caagcccggc caggacgcca acgtggtccg gcgcgttatc
     2461 gcgg
//
LOCUS       DAOSPL010000672         2459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42683_length_2459_cov_3.279534, whole genome shotgun sequence.
ACCESSION   DAOSPL010000672 DAOSPL010000000
VERSION     DAOSPL010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42683_length_2459_cov_3.279534"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..454
                     /locus_tag="PLQ54_12190"
     CDS             266..454
                     /locus_tag="PLQ54_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94068.1"
                     /translation="MPYVPPNADMARRPDETRDQWVKRVRAERGPMPEDMQRRIHAIL
                     RRSPLHPDHIPPLDKSED"
     gene            533..892
                     /locus_tag="PLQ54_12195"
     CDS             533..892
                     /locus_tag="PLQ54_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94069.1"
                     /translation="MSKESTTLAPDTSAQAWLDEPPAHAPIPCTPWCQWQDPSQEGCF
                     GPSRITPLSLEERLVDDDGCSVRYVTVYAALNRTTGAPPAVHVGLGEDRGWLMTTDEA
                     RTLAAALVAAAGEVRSL"
     gene            889..1443
                     /locus_tag="PLQ54_12200"
     CDS             889..1443
                     /locus_tag="PLQ54_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94070.1"
                     /translation="MSATPVTRTSPACPSWCDRTDQHEAERFDASESWFFHDGVILET
                     PGTRVSLSHLQPFDEQLAEVQAVTDICIEDPGKDAYTIDEARCLALSLLSACQMAAQG
                     TSSCPVWCDDHYTDPEVPGHGVHRSRDRLAGEVDFRLSGNADSPNLFASPTGRGIPVL
                     PLSGRQERDLAEMLAPWVIAVLRG"
     gene            1557..1718
                     /locus_tag="PLQ54_12205"
     CDS             1557..1718
                     /locus_tag="PLQ54_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94071.1"
                     /translation="MILPSIIAEIEDVGRYTDEELIAAFMADGESREDAEDMVLLLRQ
                     GPPGDLRLL"
     gene            complement(1887..>2459)
                     /locus_tag="PLQ54_12210"
     CDS             complement(1887..>2459)
                     /locus_tag="PLQ54_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94072.1"
                     /translation="MDATKASALAKELGISRGKVDELSTAVRNTPEGKAIKITADTSD
                     AMARIAALRSTLQSLAGQVSVAAKYAGMDKYSANAHGSIRLDGVRKMAFGDVTRNAMI
                     MARKSPILWNEAPGGESYIPLAPDKRARSLRIWQRTGELLGVGSGQSAPSISPADIRA
                     ALDGMRIDLGRVDPITHHVTGELVAAYRGA"
BASE COUNT          400 a          841 c          807 g          411 t
ORIGIN      
        1 gcggttatat cgtggggcgg gtggggcttg aacccacgac cgacggatta tgaggacgaa
       61 gccggccagg cttgtgacct gcagtgatgc ccacgaagcc ggctctgacc tgcacaaaca
      121 cccctcaacg tccatcgacg tttcccgtcg ttagtggacg ttctgcggac tatctgcgga
      181 ctcgcgggcc gcccgacaac gattgctggc caacgggccg cattgacgcc cgcacccatc
      241 cgcgcacccg aaggagtccg ttccgatgcc ctacgttccg cccaacgccg acatggcccg
      301 ccgccccgac gagacccgtg accaatgggt caagcgcgtc cgcgccgaac gcggcccgat
      361 gcccgaggac atgcagcgac ggatccacgc gatcctgcgc cgctcccccc tgcaccccga
      421 ccacatccct ccgctcgaca agtcggagga ctagcaggac ggccccggct cggtgttcca
      481 gcacccagcc ggggccctga ccgatcacca acctcagatt ggagaacgac ccatgagcaa
      541 ggaaagcacg accctcgccc ctgatacgtc cgcccaggcc tggcttgacg agccgccggc
      601 tcacgccccg atcccgtgca ccccctggtg ccagtggcag gacccttctc aggaggggtg
      661 tttcgggccg agcaggatca cgccactgtc gctggaggag cgactcgtcg acgacgacgg
      721 gtgcagcgtg aggtacgtga ccgtctacgc cgccctcaac cgaacgacgg gtgcgccccc
      781 ggccgtgcat gtcgggctcg gggaggaccg cggctggctg atgacgaccg acgaagcgcg
      841 cacgctggct gcggcgctcg tcgcggcggc tggggaggtg aggtcgctat gagcgccaca
      901 cccgtgaccc ggacctcgcc ggcgtgtccc tcctggtgtg atcgcacgga ccagcacgag
      961 gctgagcggt ttgacgcatc ggagtcgtgg ttcttccatg acggcgtgat cctagagacg
     1021 ccgggcaccc gggtgtcgct cagccacctg cagcctttcg acgagcagct cgccgaagtc
     1081 caggccgtga ccgacatctg catcgaggac ccgggcaagg acgcctacac gatcgacgaa
     1141 gcccggtgtc tggctctctc tctactgtcc gcgtgccaga tggccgccca gggcacctcg
     1201 agttgccccg tgtggtgcga cgaccactac accgaccccg aggtgcccgg gcatggggtg
     1261 cacaggtctc gggatcggct ggccggtgag gtcgacttcc ggctgtctgg aaacgcggac
     1321 tcgcccaacc tgttcgcctc cccaactggc cgaggcatac ccgttctgcc tctctcaggg
     1381 cggcaggaac gagacctggc cgagatgttg gccccctggg tgatcgctgt tctgcggggt
     1441 tgaaagcaca gctagagcgc cccccgtccc ggccgtcggg gcgggggcgc tgctgatcgg
     1501 cctacctgcc gtctttcggg gagtccggcc gtcggctggg gcctagggtg accttcatga
     1561 tccttccgtc gatcatcgcc gagattgagg atgtcggccg gtacaccgac gaggagttga
     1621 tcgctgcctt catggcggac ggcgagtccc gcgaggacgc cgaggacatg gtgctgttgc
     1681 tgcgccaggg ccctcctggc gacctgcgtt tgctctagtc caactcgggc tgattgggac
     1741 ctcgcgcgtt cggttgcggt gcggtcttgt ccctggaagg gtgggtgcgg gttgtgaaca
     1801 accccctggc gcaacacttc gtctgggcgc tcagatgccg gcgcgagagc cgtggttgcc
     1861 cctgaaacca cggacgccga ccgccatcat gccccccggt aggcggcgac cagctcgcca
     1921 gtcacatgat gggtaatcgg gtcgaccctg ccaaggtcga tgcgcatccc atcaagggct
     1981 gcgcgaatat cggccggaga gatcgagggc gcagactgtc cagagccaac accaagcagc
     2041 tcacccgtcc gctgccagat ccgcagtgat cgtgcccgct tgtcgggcgc aagggggatg
     2101 taggactccc cgcccggggc ttcgttccac aggatcgggg acttccgggc cataatcatg
     2161 gcgttgcgag tgacatcgcc gaacgccatc ttgcggacac cgtcgagccg gatcgaaccg
     2221 tgggcgttgg cagagtattt gtccatgccc gcgtacttcg cggccacaga tacctgcccg
     2281 gccagcgatt gcagcgtcga ccgcagggcg gcaatgcgag ccatcgcgtc ggaggtgtcg
     2341 gcggtgatct tgatcgcctt gccctcggga gtgttccgca cggccgtgga cagctcgtcg
     2401 accttgccgc gggagatccc caactccttg gccagcgcgg atgccttcgt ggcgtccat
//
LOCUS       DAOSPL010000673         2459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42691_length_2459_cov_2.742512, whole genome shotgun sequence.
ACCESSION   DAOSPL010000673 DAOSPL010000000
VERSION     DAOSPL010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42691_length_2459_cov_2.742512"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="PLQ54_12215"
     CDS             <1..1302
                     /locus_tag="PLQ54_12215"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOS94073.1"
                     /translation="FTSAVDEAEIARIAHARGDATGREIHETLSRAVLNHANVDLRQG
                     TLLVDLLTHDGRCVGALTFMPDRGRLAVILAPATVLATGGLGAVYARTTNPTVATGGG
                     IAAAFRAGADVMDMEFVQFHPTALATDTYPMFLISEAVRGEGAIIRDVSGVAFMREYH
                     PDGELAPRDVVARAIFDRMNRTGADHVLLDFSSLSTEEIENHFPTILRTCLSSNYDPR
                     KEPVPIAPAAHYAMGGIRVDADGRTSLPSLYACGECACTGIHGANRLASNSLLEGLVF
                     GTGSGASAASLSDEDLSHMTAVPRQISGNGSRSRGSLDVEATTQALRNLMWSACGIVR
                     SGPGLEAAIDQIREWERAMGTPAWDAAAFELCNMLTVARLIAESALCRRESRGAHFRS
                     DAPETDDENWLCRIVRRLDQAADAGDYATVEQMDPVSERAG"
     gene            1332..1904
                     /locus_tag="PLQ54_12220"
     CDS             1332..1904
                     /locus_tag="PLQ54_12220"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOS94074.1"
                     /translation="MTLHEAARAGCCETIRALLAAGADANAEVDDGWTPLHSAAKGGH
                     ADAARLLLDAGADPCRATAGGWTALHLAAKSGDAGLVELLLARGAAIDAPLAAGPTPL
                     HAAAHEGHTAVVEQLLEHDANVDATTRAGTTPLHLAAYAGHVEVVERLAARGASLDVR
                     DEWGHTPADLARFAGYVDAVELLARHAAAD"
     gene            2008..>2459
                     /locus_tag="PLQ54_12225"
     CDS             2008..>2459
                     /locus_tag="PLQ54_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOS94075.1"
                     /translation="MAEHTQYRFTAGPWNVHSGADPFGPPVRDEIDFAAKVAKLQSLG
                     YHGVQFHDDDVVSADADPATTARVAAEVKKILDDHGMVAEFVAPRLWEDPRTIDGAVT
                     SNDPRCRKYALDRSLRAVDIAKMIGTRNIVLWPAREGTYIREAKDAKAA"
BASE COUNT          393 a          894 c          806 g          366 t
ORIGIN      
        1 ttcacgtcgg cggtggacga agccgagatc gcgcgcatcg cccatgcccg cggcgacgcc
       61 acgggaagag agatccacga gactctctcg cgcgccgtcc tcaaccacgc caatgtggac
      121 ctccgccagg gcactctgct cgtggatctg ctgacacacg acggccggtg cgtcggcgcc
      181 ctgaccttca tgcccgaccg cggccggctc gccgtgatcc tggcgccggc caccgtgttg
      241 gccacgggcg gcctcggcgc cgtctacgcg cgaaccacca accccaccgt cgccaccggc
      301 ggcggcatcg ccgccgcgtt ccgcgccggc gcggatgtga tggacatgga gttcgtccag
      361 ttccacccca ccgcgctggc gaccgacacg tacccgatgt tcctgatctc cgaggcggtc
      421 cggggcgagg gggccatcat acgcgacgtc tccggcgtcg cattcatgcg cgagtaccac
      481 cccgacggcg agttggcgcc ccgcgacgtg gttgcccgag ccatcttcga ccgcatgaat
      541 cgcacggggg ccgaccacgt ccttctcgac ttctcctccc tgagcaccga ggagatcgag
      601 aaccacttcc ccacaatcct ccgcacctgc ctgagcagca actatgatcc gcgcaaggaa
      661 cccgtgccca tcgcccccgc cgctcactat gccatggggg gcatccgggt ggacgccgac
      721 ggccgcacgt cactcccgag cctctatgcc tgcggcgaat gcgcctgcac ggggattcac
      781 ggcgccaatc ggttggcctc gaactccctg ctcgagggtc tggtgtttgg caccggatcg
      841 ggcgccagtg ccgcctcgct ctctgacgag gatctgtcgc acatgaccgc ggtcccgcgc
      901 cagatctccg gcaatggcag ccgatccagg ggttcgctgg atgtggaggc caccacccag
      961 gccctgcgca acctcatgtg gtccgcatgt ggcatcgttc ggtccggccc cgggctcgaa
     1021 gcggccatcg atcagattcg cgagtgggaa cgcgccatgg gcaccccggc atgggatgcc
     1081 gcggccttcg agctgtgcaa catgctgacc gtcgcgcggc tgatcgccga atcggcgctg
     1141 tgccgcaggg agagccgtgg cgctcacttc cgctccgacg cgcccgagac tgatgatgag
     1201 aactggctgt gccggatcgt gcggcgcctc gatcaggctg ctgacgcagg ggattacgcc
     1261 acggtggagc agatggaccc ggtgtctgag cgagcaggct agtgcccgat gaccgggaag
     1321 aggtgatccc gatgacactg catgaggcgg cgcgcgcggg ctgctgtgag accatccgcg
     1381 ccctgctggc tgccggggct gacgccaatg ccgaggtgga cgacggatgg acgccgctcc
     1441 acagcgctgc caagggggga cacgcagatg ccgcgcgcct gctattggat gcgggtgccg
     1501 acccctgccg cgccacggcc ggcggatgga cggccctcca tctcgcagcc aagagcgggg
     1561 acgccggcct cgtcgaactc ctgctcgccc gcggagcggc catcgatgcg cccttggcag
     1621 cgggcccgac acccctgcac gcggccgccc acgagggcca cacggctgtg gtcgagcagc
     1681 ttctcgagca cgacgcgaac gttgatgcca ccacccgcgc cggcaccacg ccgctgcact
     1741 tggccgcata cgccggtcac gtcgaggtcg tcgagcgcct ggccgcccga ggcgcgtccc
     1801 tggacgtccg ggatgagtgg ggccacacgc ctgcggatct ggctcgcttc gccggctacg
     1861 tcgacgccgt cgagttgctc gcccgtcacg ccgctgccga ctagccgcgc cccgggggag
     1921 gattcggcgg caacgatgcg ggatacaccg tcagggcgcg ccgctgccta gtctcggcgc
     1981 cctggacccg ttattgggga ggtcgttatg gcggaacaca ctcagtaccg attcaccgca
     2041 ggaccctgga atgtccacag cggcgcggat cccttcgggc ccccggtccg cgacgagatc
     2101 gacttcgccg ctaaggtggc gaaactccag tccctgggat accacggcgt gcagttccac
     2161 gacgacgacg tcgtgagtgc cgatgctgac ccagccacca ccgcccgagt ggcggccgag
     2221 gtcaagaaga tcctggacga ccacggcatg gtcgccgagt tcgtggcacc ccgactgtgg
     2281 gaggatcccc gaaccatcga cggcgccgtc acgtccaacg atccccggtg ccgaaagtac
     2341 gcgctcgacc gctccctccg agccgtcgat atcgccaaga tgataggcac ccgcaacatc
     2401 gtgctctggc ccgcccgtga gggcacctac atccgcgagg ccaaggacgc caaggcggc
//
LOCUS       DAOSPL010000674         2456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42771_length_2456_cov_2.332362, whole genome shotgun sequence.
ACCESSION   DAOSPL010000674 DAOSPL010000000
VERSION     DAOSPL010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42771_length_2456_cov_2.332362"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PLQ54_12230"
     CDS             complement(<1..550)
                     /locus_tag="PLQ54_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94076.1"
                     /translation="MSHARFALLCVVSWLGSGVCALGVDTPLEPFASDGHTVFLYHFD
                     EGQGGVARDSGPHGYEAEVRGAQWAPGRFGSALRFDGRDDSVFRDVPEAARGLAAFTI
                     ECWFQPHAPQGRRFLVGQDVGFHFEVDDGGASALSLYNQGGGVPNADGVSHRQVGVPT
                     GPLRLGRWHHLADAGAHGAGRLG"
     gene            complement(644..919)
                     /locus_tag="PLQ54_12235"
     CDS             complement(644..919)
                     /locus_tag="PLQ54_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94077.1"
                     /translation="MSEERLSPEEVILQLRMAGNANWTECYRSLLSDTEAACIRDEAT
                     RFLDRNTPGAWADARRVVGQVCGPSPEGFRALFEAVTVMRARGPEAW"
     gene            complement(1145..1654)
                     /locus_tag="PLQ54_12240"
     CDS             complement(1145..1654)
                     /locus_tag="PLQ54_12240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS94078.1"
                     /translation="MALGDRAAVCVMRAGEADLPEILAVQKRAFASEAERYGDPNLPP
                     LRETLDEIREQLATHVVLKAVWDCQIVGSVRGHLEAGSCHVTRLAVRPDVQGRGIGSL
                     LVGALEEQFPNAESFVLFTGHKSHRTIALYERLGYRVTHSTVESDRVTLVHMAKHSGS
                     ERPGAERQG"
     gene            complement(1699..>2456)
                     /locus_tag="PLQ54_12245"
     CDS             complement(1699..>2456)
                     /locus_tag="PLQ54_12245"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOS94079.1"
                     /translation="ADPFPRIERGQGEPVVLLPGMFGQPSNWQHQLDHLSDGYRVITL
                     DLPLFDPGFSECTVHGLGDYVRRFLDWAGIARAVLVGNSLGGHVALDIATRYPSRVRA
                     MVLAGSSGLFERGFERGVPTRPDRAWIETRIREVFCHESSIPAGQGDEIERIVQSRAN
                     KLRILHWAKSAKRTHMGALLSRIAAPTLLIWGKQDSITPLEVAVEFLRGIRHSELVLF
                     NECGHAPMMEHPARFTEVLRHFLHRHAPVACDA"
BASE COUNT          444 a          864 c          802 g          346 t
ORIGIN      
        1 caccaagccg gcccgctcca tgcgctccag catcggcgag gtggtgccat cgcccgaggc
       61 gcagcgggcc ggtgggaact ccgacctggc gatgcgagac accgtcggcg ttggggacgc
      121 cgccgccctg gttgtagagc gagagggcgg aagcgccgcc gtcgtcgacc tcgaagtgga
      181 aacccacgtc ctggccgacg aggaacctgc ggccctgggg ggcgtgaggc tggaaccagc
      241 actcgatggt gaacgcggcc aggccgcgtg cggcctcggg gacatcgcgg aaaacgctgt
      301 cgtcgcgccc atcgaaccgg agggccgagc cgaaacgccc gggtgcccac tgggccccgc
      361 gcacctcggc ctcatagccg tgcgggcccg aatcgcgggc cacaccgccc tggccctcgt
      421 cgaagtggta gaggaaaacg gtgtggccgt cggaggcaaa gggctcgagc ggcgtatcaa
      481 cacccagggc gcacacgccg gacccgagcc acgagacaac acacagcaga gcgaaccttg
      541 catggctcac tgcgaccccc atgcccggca tcagctgggt gaccgatgtc tccgagcaca
      601 ggcaggcttc tggcgggacg gggcgatacc tgccgcggag cgtctaccag gcttcggggc
      661 ctcgcgcccg catgacggtg acggcctcga acagggcccg gaagccctca gggctcggcc
      721 cacacacctg gccgacgacg cgtcgcgcgt cggcccaggc ccccggggtg ttgcggtcca
      781 ggaaacgagt ggcctcgtcg cggatgcagg ccgcctccgt gtcagagagc aagctccgat
      841 agcattcagt ccagttggca ttccccgcca tccggagttg taggatgact tcctcgggcg
      901 agagccgctc ttcggacatg cgatcctcct cgtcggtgcg gacgagcccc caccgctgcg
      961 ctcgtccaac aacacaacac ccacaccatg gggccaagag tccgggagag cagggcgatc
     1021 cacccacacc gggctaccca gttcggggca gcatccgaga cgatcggtga ggacgatgcg
     1081 tcgcgctgga gccgccgtac acctcgtgat tccccatggc cccggcgttt ccttccgccg
     1141 tgggctaccc ctgccgctcc gcgcccggcc gttccgagcc ggagtgttta gccatatgca
     1201 ccagagtcac ccggtcactc tcgactgtcg agtgagtgac acgatagccc agacgttcat
     1261 agagggcgat ggttcggtgg ctcttgtgcc ccgtgaatag cacgaaggac tcggcattcg
     1321 ggaactgctc ctccagcgcc ccaacgagca gggacccgat accgcggccc tgaacgtccg
     1381 gacgcaccgc cagccgtgtc acgtggcatg aaccggcctc gaggtgcccc cgcacggatc
     1441 caacgatctg gcagtcccac accgccttca gcacgacgtg cgtcgcgagc tgctcgcgga
     1501 tctcgtcgag agtctcacgc agaggcggga ggttggggtc gccgtagcgc tcggcctcac
     1561 tcgcaaacgc tcgcttctgg acggccagaa tctcaggaag gtcggcctca ccggcccgca
     1621 tgacacatac cgcggccctg tcgcccaacg ccacgagccc ctcactccca tgccgccgcg
     1681 ccctcggtca gacgaccact acgcatcaca agccaccggg gcgtgccggt gcaggaagtg
     1741 ccggagaacc tcggtgaaac gcgcgggatg ctccatcatg ggcgcgtggc cgcactcgtt
     1801 gaacagcacg agctccgagt ggcggatgcc acgcaggaac tccaccgcca cctcgagagg
     1861 tgtgatgctg tcctgcttgc cccagatcaa gagcgtcggc gcggcgattc gactcagcaa
     1921 cgcgcccatg tgagtccgct tggcggactt ggcccagtgc aatatgcgca gcttgttggc
     1981 ccgggactgc acgatccgtt cgatctcatc gccctgcccg gccgggatgc tcgattcatg
     2041 acagaagacc tcgcggattc gagtctcgat ccacgcccga tcgggccggg tggggacgcc
     2101 ccgctcgaag ccgcgctcga aaaggccgga ggacccggcg agaaccatcg ctcggacgcg
     2161 cgaggggtac cgcgtggcga tgtcgagcgc cacatgcccc cccagcgagt tcccaaccag
     2221 caccgcacgc gcgatgcccg cccagtccag gaagcgccga acgtagtcac ccaagccgtg
     2281 aaccgtacac tcactgaagc ccgggtcgaa cagcggcaga tccagagtga tgacacggta
     2341 gccgtcggac aggtgatcca gctggtgctg ccagttggag ggctgcccga acatgcccgg
     2401 taggagaacg accggttcgc cctggccgcg ctcgatccgc gggaacgggt cggcag
//
LOCUS       DAOSPL010000675         2455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42789_length_2455_cov_3.058750, whole genome shotgun sequence.
ACCESSION   DAOSPL010000675 DAOSPL010000000
VERSION     DAOSPL010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42789_length_2455_cov_3.058750"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PLQ54_12250"
     CDS             <1..697
                     /locus_tag="PLQ54_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOS94080.1"
                     /translation="TLIELLVVIAIIAILAAILFPVFAKARAKARQATCQSNLKQIGL
                     ACGMYQQDYDGIYVPVAVWNSNYAGNGVWWMMLVEPYMKNLQLLLCPSYGCNYGGIGS
                     WTGAGFCGDTPGGTSTCDQPPRSRFVGGYGINWGRNDLNAAWQGPAGRKDDLVRAPGD
                     TILVSESHCIVAVHSTMGWPGQSRCRGIPPHNEGINQLYCDGHVKWVKIQTDAAGNLV
                     STEPVGKWIITDD"
     gene            1043..1225
                     /locus_tag="PLQ54_12255"
     CDS             1043..1225
                     /locus_tag="PLQ54_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94081.1"
                     /translation="MRRAVALVVCAWVVLSLVLACGCKSGSSSGGGKTPEKAREMMLK
                     ATGGKGLMPSKSGTGE"
     gene            1241..1966
                     /locus_tag="PLQ54_12260"
     CDS             1241..1966
                     /locus_tag="PLQ54_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOS94082.1"
                     /translation="MREVGVMMKRSRGFTLIELLVVIAIIAILAAILFPVFAKARAKA
                     RQATCQSNMKQIGLACGMYQQDYDGIYVPTNVRNSNYPGNNGYWWSSLIEPYMKNIQL
                     LLCPSYGCNFGGIGTWTGAGYCGDTAGGTSTCDQPPRARFVGGYGINRGSDWQGPAGR
                     TDDAVRAPGDTILISESMCIVASHSTFGWPGNAKCRGIPPHNDGINQLYCDGHVKWVK
                     VQTDAAGNLVSTESVYKWTVNDD"
     gene            complement(2164..>2455)
                     /locus_tag="PLQ54_12265"
     CDS             complement(2164..>2455)
                     /locus_tag="PLQ54_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94083.1"
                     /translation="PSVLTRTFEVPKRKKTELRLTVGHHPEGDWLLQVKVDGQVVLEQ
                     PVTQETAPETWTEVEVDLSRYAGKTVRLELVNQPTGWMCEAAYWARVAIESR"
BASE COUNT          460 a          791 c          791 g          413 t
ORIGIN      
        1 taccctgatc gaactgctcg tcgtgattgc gattatcgcg atcctagcag cgatcctctt
       61 cccggtcttc gccaaagcac gggcgaaggc gcgccaggcc acctgccagt ccaacctgaa
      121 gcaaatcggg ctggcgtgcg gtatgtacca gcaggactac gacggcatct acgttccggt
      181 ggccgtgtgg aactccaact acgccgggaa cggcgtgtgg tggatgatgc tcgtcgagcc
      241 ctacatgaag aacctacagc tcctgctctg ccccagctac ggctgcaact atgggggcat
      301 tggctcgtgg acgggtgcgg ggttctgcgg cgacacgccg ggcgggacgt cgacctgcga
      361 ccagccgccc cgctcgcggt ttgtcggcgg ctacggcatc aactggggcc gcaatgacct
      421 caacgcagcc tggcaaggcc cggcgggacg caaggacgac ctggtccgag ctcccggcga
      481 caccatcctg gtcagtgaga gccactgcat cgtggccgtg cactcgacca tgggctggcc
      541 aggacagagc cggtgccgag gcatcccgcc gcacaacgag gggatcaacc agctctactg
      601 cgacggccat gtgaagtggg tcaagataca gaccgatgct gccggcaacc tcgtgtcaac
      661 cgagccggta ggaaagtgga tcatcaccga tgactaggta gcataccggc ccatccgggc
      721 cgtccgacct atccgcactt ctgacgacag aacagccagt ctggcgtact cgtgacgcca
      781 gccccgctac aggcggccgc tccgatgacc tttcggagcg gccgcagtcg cttccaggcc
      841 gggaggtcta tcgaggtcgg cacaagggac gacagcgtac cgccgcgagg gcctgccggt
      901 tgtgccccaa tgaccccgaa ccatgagtat cctcatagat tttatgagac ggaatgcaga
      961 tgaaaagcaa agcgttctac gtgttgccga cagggagatc cagcgcgagc ggcgaactgt
     1021 acctcgagag gaggtgacgc acgtgcgtcg agccgtcgcg cttgtcgtgt gcgcgtgggt
     1081 ggtgctgagc ttggttctgg cctgcggctg caagtccggc agctcctcag gcggtgggaa
     1141 gactcctgag aaagccagag agatgatgct caaggcaaca ggcggcaagg gtctcatgcc
     1201 ctccaagagt gggaccggtg agtagcagga agcgtccgga atgcgggagg tcggcgtaat
     1261 gatgaagcgg tcgcgaggtt tcactctgat tgagctactc gtggtcatcg cgatcatcgc
     1321 gattctcgcg gcgatcctct tcccggtctt cgctaaggcg cgcgcgaagg cccgtcaggc
     1381 cacctgccag tcgaacatga agcagatcgg cttggcttgc ggcatgtacc agcaggacta
     1441 cgacggcatc tacgtgccca ccaacgtccg gaactccaac tacccgggca acaatggcta
     1501 ctggtggtcg tctctgatcg aaccctacat gaagaacatc cagctcctgc tctgcccgag
     1561 ctatgggtgc aacttcggtg gcatcgggac ctggacgggt gcgggctact gcggcgacac
     1621 ggccggcggg acctcgacgt gcgaccaacc cccacgggca cggttcgtcg gcgggtatgg
     1681 catcaaccgg ggctcggact ggcagggccc cgccggcagg accgatgacg ccgtacgagc
     1741 tccgggcgac acgatactca tcagcgagag catgtgtatc gtggcatcgc actccacgtt
     1801 cggctggccg ggcaacgcaa agtgccgagg catccccccg cacaacgacg ggatcaacca
     1861 gctctattgc gatgggcacg tgaagtgggt caaggtccag accgacgccg ctgggaacct
     1921 cgtatccacg gagtcggtct acaagtggac cgtcaacgac gactagcgaa cgggcccccc
     1981 tgaccccgcc gggcacctcg ggctgagcca ccccggcgcg ctgcgatgcc tcggggaaga
     2041 acagcgtttc gatgagaacg gccgccctgg ccgatcgccg gggcggccgt ctgcctgcct
     2101 ccagctacgg cgcgccgtgg gtagccaccg cccaggtgga gcggcccgac ggacccttgt
     2161 cctctaccgg ctctcgatag ccactcgtgc ccagtaagcg gcctcgcaca tccagcccgt
     2221 cggttggttc acgagctcca gcctcacggt cttgccggcg tagcgcgaga gatcgacctc
     2281 gacttcggtc cacgtctcgg gcgccgtctc ctgagtcacg ggctgctcga gaaccacttg
     2341 gccgtcgacc ttcacctgca gcagccagtc tccctcggga tggtgcccga ccgtgagccg
     2401 aagctcggtc ttctttcgct tcggcacctc gaaggtgcgg gtgaggacgc tgggg
//
LOCUS       DAOSPL010000676         2452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42860_length_2452_cov_3.289111, whole genome shotgun sequence.
ACCESSION   DAOSPL010000676 DAOSPL010000000
VERSION     DAOSPL010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42860_length_2452_cov_3.289111"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..682)
                     /locus_tag="PLQ54_12270"
     CDS             complement(185..682)
                     /locus_tag="PLQ54_12270"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOS94084.1"
                     /translation="MSGERVPAERTMVFDRIVDLLTRHGADFRVMEHEPVRTSEQAAR
                     VRGTPLERGAKALVLDTGGDLVMAVISAARRTDFGKLKRHLGSRRVQLASAEAVMDRT
                     GCLPGGVPPFGNLFGLRVLMDPSLRAVDRIDFNAGERARSMDLRREDYERIVRPEVVD
                     FAADL"
     gene            complement(679..>2452)
                     /locus_tag="PLQ54_12275"
     CDS             complement(679..>2452)
                     /locus_tag="PLQ54_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94085.1"
                     /translation="WLAVDDSATVYLNGELLGEVGGWSDVKPFADLHGRLRPGANVLS
                     AEARNGSGPAGLLARLELTTPQGTHLVTTDEHWVTFGSQPAEWPAAATGGAPANPLGD
                     PATSLWAGTMTDWPTLRNGRLLFTRTPLEPGAWVSNTISVRVEPRTLHTMVRDPDHLV
                     GMQWEPWFTPHNCNWTTAQAVPVVGKFWSWDPDALRQHMIWLMESGIDFLIVDWTNHL
                     WGKSHWDERDDSPNEIIHCTTLALETLASLRDEGLPAPAVVLYPGLNNGPATITEAIN
                     EELDWIYHNYVRNPRFRGLFVEYLGKPLVLIHNGSGPSGLEGQAPVRDDHFTVRWQSS
                     QSDRTRLNESGYWSWMDGSLVPALTTYEGQPEALTVSTAFFSSGGWLAPTAYGRRGGW
                     TYIETFKAALEHRPRFLEIHQFNEFAGQPEGNGYGDEHNVYVDSYSVELSDDIEPVSL
                     TAPAYRGNGGWGFLYLNLTRALIDLYRQARPETTVVAIASPLRKQTVRADSVRAIWNW
                     VGVEPESFSVAANGQTLVTGLEGTEADVDLSAFPDGPVHLVLTANGTRSRYLLSDTED
                     SLPLEQLVPASASVDFVLERERKR"
BASE COUNT          411 a          798 c          835 g          408 t
ORIGIN      
        1 accggtgtgc cccccggttt cccaggagcc ctgccctgtg agcgtccacc ccgagggggt
       61 cgtgtctcct tcctcgaatg acgggctggg cacgaccggc tcctgggccc aggctagcgg
      121 cgccagaccg acggcgcaca agggaaccca ccatttggcc atctgcggca acccctctca
      181 cgggttacag atccgccgcg aagtcgacaa cctcggggcg cacgatgcgt tcgtagtcct
      241 ctctccgcag gtccatggag cgcgcgcgct cgccggcgtt gaagtcgata cggtccaccg
      301 cccgcagcga cgggtccatg agaacgcgga ggccaaacag gtttccgaag ggcggaacac
      361 cgccgggcag gcacccggtt cggtccatga cggcctcggc gctggcgagc tggacgcggc
      421 gcgagcccag gtgcctcttg agcttcccaa aatcggtgcg gcgcgcggcc gagatcacgg
      481 ccatgaccag gtccccgccc gtgtcgagca ccagcgcctt ggcgccacgc tcgaggggcg
      541 tgccgcgcac ccgagcggcc tgctcgctgg tgcgaactgg ttcgtgctcc atgacgcgaa
      601 agtccgcgcc atggcgtgtg agcagatcga cgatacggtc gaacaccatg gtgcgttccg
      661 caggcacccg ctcgccgctc accgcttgcg ctcccgttcg agcacgaagt cgaccgatgc
      721 cgacgcgggc acgagttgct cgagcggcag tgagtcctcg gtgtcgctga gcagatagcg
      781 cgaccgtgtg ccgttggcgg tcagtaccag atgcactgga ccatcgggaa aggcggagag
      841 gtccacatcg gcctcggtgc cctccagccc ggtgacgagg gtctgtccgt tggccgccac
      901 ggagaacgac tcgggctcga cgcccaccca attccagatg gcgcgcacgg agtccgcccg
      961 cacggtctgc ttccgcagtg ggcttgcgat agccaccacc gtggtctccg gtctcgcctg
     1021 acggtagagg tcgatcaggg cgcgagtcag gttcagatag aggaagcccc atccgccgtt
     1081 gccacggtag gcgggggcgg tcagcgagac gggttcgata tcatcgctga gctcgacgct
     1141 gtaggagtcg acatagacat tgtgctcgtc accatagccg ttgccctcgg gctggccggc
     1201 gaactcattg aactggtgga tctcgaggaa ccgcggccga tgctcgagcg ccgctttgaa
     1261 tgtctcgatg tacgtccagc cgccccggcg gccataggca gtgggggcga gccacccgcc
     1321 cgagctgaag aaggccgtcg agaccgtcag tgcctcgggc tgaccctcat atgtggtcaa
     1381 ggcgggcacc aggctgccat ccatccacga ccagtagccg gattcgttca gcctcgtccg
     1441 atccgactgt gaggattgcc agcgaacggt gaaatggtca tcgcggacag gagcctggcc
     1501 ttcgaggccg ctcgggccgc tgccgttgtg gatcagcacc aggggcttgc ccaggtactc
     1561 gacgaaaagc ccacggaagc gggggttgcg gacgtagttg tggtagatcc agtcgagctc
     1621 ctcgttgatg gcttcggtga tcgtcgctgg accgttgttc aggcccgggt agagcaccac
     1681 cgctggcgcg gggaggccct catcgcgcag cgaggccaga gtctcgaggg ccagggtggt
     1741 gcagtggatg atctcgttgg ggctgtcgtc gcgctcatcc cagtggctct tgccccacag
     1801 gtggtttgtc cagtcgacga tgaggaagtc gatccccgac tccatgagcc agatcatgtg
     1861 ctggcgcagt gcgtcggggt cccacgacca gaacttcccg actaccggca cggcctgagc
     1921 agtagtccag ttgcagttgt ggggcgtgaa ccagggctcc cactgcatgc cgacgagatg
     1981 atcgggatcc cgcaccatcg tgtgcagagt gcgtggctcg acgcggacgc tgatggtgtt
     2041 cgagacccat gcaccgggct ctagcggcgt tcgggtgaac aacaaccggc cattgcgcag
     2101 cgtgggccaa tccgtcatgg ttccggccca gagcgaagtc gcagggtctc cgagggggtt
     2161 cgccggcgcg ccacccgtgg ccgcagcggg ccattccgca ggctggctgc cgaacgtgac
     2221 ccagtgctca tcggtggtaa cgagatgagt gccctggggc gtggtcagct cgaggcgtgc
     2281 gaggagacct gccggcccgg agccgttccg tgcctcggcc gagagaacgt tcgcacccgg
     2341 ccgtaatcgc ccatggaggt cggcgaaggg cttcacgtca ctccagcccc cgacctcgcc
     2401 gagaagctcg ccgttcagat agaccgtggc cgaatcgtcg acggcgagcc ag
//
LOCUS       DAOSPL010000677         2452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42865_length_2452_cov_2.619524, whole genome shotgun sequence.
ACCESSION   DAOSPL010000677 DAOSPL010000000
VERSION     DAOSPL010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42865_length_2452_cov_2.619524"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1827)
                     /locus_tag="PLQ54_12280"
     CDS             complement(<1..1827)
                     /locus_tag="PLQ54_12280"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS94086.1"
                     /translation="MLGFDLTAIDRQGYDLVMESAYRVVVAGGGPTGLSAAISLADSG
                     VAVTLIEQSDGLGGHARELGCKATDRCQACGACLAAPLVREVRQHGLIDLITSGRIVN
                     EELVGGRHRFTVAAPAGSVSVEADSLVLAGGYSTIDLSRYRQEYGHGFAPGVATTLEL
                     ESEIRLRGPAGLTGRRFAFIQCVGSRDKALGRDYCSRVCCASSLRLAQLIRTERPDSE
                     VTVFYQDLQPSGRDFGALVASCEALGVVLVRGLPSKVRWDPVVGSTVVCVADTLAGAA
                     RELPFDRVVLAVGQWGPGGAEVWPGRCLSVDETGFACPEQPNGAFAAGTMTGPMGLAE
                     AIAEGKLAAACVLERLGHPLAELKDAGPDADFAPPDDPRQLGLLLNLPGLEESRPPGV
                     PEEALGTAGLPAGLTGRTVFLMDLGGPFNRAAHLRAMEAAAALGSQAAYVYRHAYTAE
                     MGLERLYNEMRRAGVGVHPYTETPELGDGRLRFRDPLVGLPVEIAYDHLVVGEQAGPS
                     PANREFHDRLGLPLDANGHVFPSNPHLGPVGTPRRGLFALDNGRGVLGVRGFEARARA
                     LARAAATVDVRKGSAKVDPDKCVSCLTCLRVCPHDAPEILRCQ"
     gene            complement(1842..2318)
                     /locus_tag="PLQ54_12285"
     CDS             complement(1842..2318)
                     /locus_tag="PLQ54_12285"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS94087.1"
                     /translation="MVIDGDPGLNDALLGAMRYLKSGDHEGALDALRRALILAPENAT
                     VHAALGNALIAAGRHAEATEAHRRALEITPECPQAYIGLAMTCFARGEDASGRIILDQ
                     AARVTERATAGGIAPTADEILDEYAAAVSLPDPERAAIMARLRGLSRERLYRRAFA"
BASE COUNT          443 a          921 c          760 g          328 t
ORIGIN      
        1 ctggcagcgc agaatctccg gcgcgtcatg tgggcatacg cgaaggcatg tcagacagga
       61 cacgcacttg tccggatcga ccttagccga acccttccgc acatcgacgg tggccgcggc
      121 ccgtgccagc gctcgggccc tggcctcgaa gcccctcaca cccaaaacgc cgcgtccgtt
      181 gtccagcgcg aagaggcccc gccgcggagt gcccaccggc ccgaggtgag ggttgctcgg
      241 gaacacgtgg ccattcgcgt ccaacggcag ccccagccgg tcgtgaaact cgcggttcgc
      301 cggcgacggg cccgcctgct ctcccaccac cagatggtca tacgcgatct ccaccggcaa
      361 gcccaccagc gggtcccgga aacgaaggcg cccatcgccc aactccggcg tctccgtgta
      421 gggatgtacc ccaacgcccg cgcgccgcat ctcgttgtag agccgctcga gccccatctc
      481 cgccgtgtat gcatgccggt acacgtaggc ggcctggctc cccagcgcag cggcggcctc
      541 catcgcgcgc aggtgtgcgg cccggttgaa cggtcctccc aggtccatca ggaaaacagt
      601 ccggccagtc agacctgctg gcagaccggc cgtccccagc gcctcttccg gcacccccgg
      661 gggccgcgat tcctcgagtc ccggtaagtt cagcagaaga cccagctgtc gaggatcgtc
      721 aggaggcgcg aagtccgcat cgggccccgc atccttcagc tccgccaggg ggtgcccgag
      781 ccgttcgaga acgcaagccg cagccaactt gccctcggca atggcctcgg ccagccccat
      841 gggcccggtc atcgtcccgg cggcgaaagc accattgggc tgctccggac acgcgaatcc
      901 agtctcgtcc accgataggc agcggcccgg ccagacctca gcgcctcccg gaccccactg
      961 gcccacggcc aggaccaccc ggtcgaatgg gagttcccgc gccgcgcccg ccagcgtatc
     1021 ggccacgcac accacggtgg agccaacgac gggatcccac cggactttgg aaggcagccc
     1081 acgaaccagt acaaccccca gagcctcgca cgatgccacc aatgccccaa agtcacggcc
     1141 cgatggctgc aggtcctggt agaagaccgt gacctcggaa tccggccgct cggtccggat
     1201 gagctgcgcg agacggagac tcgaagcgca gcacacccga gagcagtagt cgcggcccag
     1261 ggccttgtcc cgactcccga cacactggat gaacgcgaaa cgccggcccg tcaggcccgc
     1321 gggcccacgc aatcggatct ccgactccag ctccagcgtg gtggccactc cgggcgcgaa
     1381 gccatgcccg tactcctggc ggtaccgtga aagatcgatg gtcgagtagc cgccagccag
     1441 aaccagcgag tcggcctcga cagacacaga acccgccggg gcggccaccg tgaatcggtg
     1501 ccggccccca accagttcct cattcacgat gcggcccgag gtgatgagat cgatgaggcc
     1561 gtgttgacgg acctcgcgga ccagaggggc cgccaggcat gccccacagg cctggcatcg
     1621 atcggtagcc ttgcagccga gctctcgagc gtgcccaccc aagccgtccg actgctcgat
     1681 cagcgtcacg gccacgccag agtcagcgag ggaaatggct gcggaaagac cagtggggcc
     1741 gccgcctgcg accacgaccc ggtacgcgga ctccatcacg aggtcatacc cctggcgatc
     1801 gatggcggtg aggtcgaagc ccaacacggg cggcaccggg actaggcgaa cgccctgcga
     1861 tacaggcgct cccgcgacag gccccgaagc cgcgccatta tagccgcgcg ctccggatca
     1921 ggcaacgaaa ccgcagccgc atactcatcg agaatctcgt cggccgtcgg agcgataccg
     1981 cctgctgtcg cgcgctcggt cactcgggcc gcctggtcca ggatgatgcg cccggaggcg
     2041 tcttcgcccc gggcaaagca ggtcatcgcc agcccaatgt aggcctgggg acactccgga
     2101 gtgatctcca gcgcccgacg gtgtgcttcg gtggcctccg cgtgacgccc ggcggcgatc
     2161 agcgcgttcc cgagagccgc gtggaccgtg gcgttctctg gggccaggat gagcgcccgc
     2221 cgcagcgcat ccaacgcccc ttcgtggtcc cctgacttca gataccgcat cgcgcccaaa
     2281 agtgcatcat tcagccccgg gtccccatca atcaccatag cgccccccgc tgtcgaacac
     2341 tgcgcacact ctgatgataa cgccaatgca acgggaatgc gaacgggatc tgccgcgtcc
     2401 gcccccgcgt cgccgggccg gcggctccgg ggcgcccgcg cggcagccgc at
//
LOCUS       DAOSPL010000678         2449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42927_length_2449_cov_2.645363, whole genome shotgun sequence.
ACCESSION   DAOSPL010000678 DAOSPL010000000
VERSION     DAOSPL010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42927_length_2449_cov_2.645363"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PLQ54_12290"
     CDS             <1..542
                     /locus_tag="PLQ54_12290"
                     /inference="COORDINATES: protein motif:HMM:NF026058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOS94088.1"
                     /translation="DNGPWLTQNQQGGCAGLLRGGKGSTWEGGLREPTLAVWPGTIPP
                     GRVAMDLGTTLDVFPTCAELAGVPVPSDRVMDGVSLVPVLTAFRPGSRDTVFYYSGSE
                     LRAVRHGSWKAHYVTHSGYRDDREVHDPPLLFHLDVDPGERYDVAKEHPAILDRIAAV
                     AALHRAAAEVAPSQLDLPA"
     gene            720..1829
                     /locus_tag="PLQ54_12295"
     CDS             720..1829
                     /locus_tag="PLQ54_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS94089.1"
                     /translation="MDHQRVTRRAFLARTTAAGAGLAAAIGFGLRGASAQQEPEKRNM
                     QTSMTYRMLGDTNLLVSCLSFGCIQLQDDRLAVLDLAVERGVNTVHVNRGYNGGQAIA
                     SLGKFLKDKRDKVWVMLKSNSPNIDADLETLQTDHVDILCVHDLNPEQLRDPAIADKF
                     QKLVDAGKTRFLNLTSHSDPQQCMESAVDCGMYTSVLATINPSNLDAFRPTLSKAMDR
                     KVGTFTMKCMQNAGGEGPAQVTRAMLAAGVTSVLKTLSSNEEAEAFLGAVAEEVEIAR
                     AGAVTVASAAGCTLCGACTRSCPQNVAIQDLLRNYEYYCVQQGKPAIGREQFAQLPAV
                     RTAAHCGDCGTCERVCPQRLPVRRLIREADRVLLA"
     gene            complement(1914..>2449)
                     /locus_tag="PLQ54_12300"
     CDS             complement(1914..>2449)
                     /locus_tag="PLQ54_12300"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOS94090.1"
                     /translation="MGPVGGGRLAGLSPELARMALSPSASTPALALRFVLSNPAISTA
                     ISGMSTIQMVDENVATASRDDMLSADEREQVRAALEEAERIAKLYCTGCRYCMPCPHG
                     VDIPGNFSLMNYYRVYGLTEYAKTHYAHKPAQREAGDERCRLQASACVECGECEPKCP
                     QHIPIIEQLKETHAALG"
BASE COUNT          428 a          846 c          765 g          410 t
ORIGIN      
        1 gcgacaacgg cccctggctg acccagaatc agcaaggtgg ctgcgcgggt ctgctgcgcg
       61 gtggcaaggg cagcacctgg gaggggggtc tgcgcgagcc cacgctcgcc gtatggcccg
      121 gcacaatacc cccgggccgc gtcgccatgg atctcggtac cacactcgac gtgttcccca
      181 catgcgcgga gttggccggg gtccccgtgc catccgatcg ggttatggac ggtgtcagcc
      241 tcgtcccggt gctgacggca ttccgtcctg gatctcgcga caccgtgttc tactactcgg
      301 gatccgagct ccgggccgtc cggcatggca gctggaaagc ccactacgtg acccactcgg
      361 gctaccgcga cgaccgggag gtgcatgatc cgccgctctt gttccacctc gatgtcgacc
      421 ccggggagcg gtacgacgtg gcgaaagagc atcccgccat tctggaccgg atcgcggcag
      481 tggccgcgct ccaccgtgcg gccgcagaag ttgcgccgtc gcagctcgac ctcccggcgt
      541 agcgtctccg ggccgccaca gtgcacagag gcggttccgg ggcgtcccgc ggacggagtc
      601 cccgggaacc gagccgccgt tggcaatgct gcgtgctcaa ggaggacgcc atcgcggcat
      661 gtccaatacg tgacgctgtg tgccgcagta ccccatgacc ctgtgaaggg aacgaatgca
      721 tggaccatca gcgtgtcacc cgtagagctt tcctcgccag gacgacggcc gccggagccg
      781 gcctcgcagc cgccattggt ttcgggctca gaggcgcttc ggcccagcag gagcccgaga
      841 agcgcaacat gcagacgtcc atgacctaca ggatgctagg ggacacaaat ctgttagtaa
      901 gctgcctatc tttcggctgc atccagctcc aggatgatcg tctcgccgtg ctcgatctgg
      961 ccgtcgagcg gggcgtcaac acggtccacg tcaaccgcgg ctacaacggc ggccaggcca
     1021 tcgcatcgct cggcaagttc ctcaaggaca agcgcgacaa ggtctgggtc atgctcaaga
     1081 gcaactcccc aaacatcgat gccgacttgg agacactcca gaccgaccat gtcgacatcc
     1141 tgtgcgtgca tgacctgaac cccgaacagc tcagggaccc ggccatcgcc gacaagttcc
     1201 agaagctcgt cgacgcgggc aagacccggt tcctgaacct cacatcccac tcggatccac
     1261 agcagtgcat ggaatcggcc gtggactgcg ggatgtatac ctcggtgctg gccaccatca
     1321 acccgagcaa cctcgatgcc ttccggccca cgctctcgaa ggcgatggat cgcaaggtcg
     1381 gcaccttcac aatgaagtgt atgcagaatg cggggggcga agggccggcg caggtcaccc
     1441 gggccatgct cgccgcgggc gtgacgtcag tcctgaagac cctcagctcc aacgaggagg
     1501 ccgaggcgtt cctcggtgcc gtcgctgagg aggtcgagat cgctcgcgcc ggtgccgtga
     1561 ccgtcgcttc ggccgccgga tgcaccctct gcggcgcctg caccagaagc tgcccgcaga
     1621 atgtggccat ccaggacttg ctgcgcaact acgagtacta ctgcgtccaa cagggcaagc
     1681 ccgcgatcgg ccgcgagcag ttcgcccaac tacccgccgt ccgcaccgcc gcacactgcg
     1741 gggactgcgg cacctgtgag cgcgtctgcc cgcaacgctt gccggtccgg cgcctcatcc
     1801 gcgaggccga tcgcgtgctg ctcgcctagc gttgctgcgt cgtcgaaatc agaggagcgg
     1861 ctacctcatc ggtcgccgct cctctccatg tccttgccgc agatcctggc gccctacccc
     1921 aaggccgcat gcgtctcttt gagctgctcg atgatcggga tgtgctgcgg gcacttgggt
     1981 tcgcactcgc cgcactccac acatgcgctt gcctggaggc gacaccgctc atcgccggcc
     2041 tcacgctgcg cgggcttgtg ggcgtagtgg gtctttgcat actcggtaag cccgtagaca
     2101 cgataatagt tcatcaaact aaagttgccg ggaatgtcca ctccatgcgg gcacggcatg
     2161 cagtagcggc atccggtgca gtaaagcttt gcgatccgtt cggcctcctc cagcgccgca
     2221 cgcacctgtt cgcgctcatc agccgacagc atgtcgtcgc gcgatgccgt cgccacgttc
     2281 tcgtcgacca tctggatggt gctcatgccc gagatggccg tcgagatcgc cggattcgag
     2341 agcacgaacc gcagcgccag ggccggagtg ctcgccgaag ggctcagcgc cattctggcg
     2401 agctcgggcg acagcccggc cagccggccg ccccccacgg ggcccatgg
//
LOCUS       DAOSPL010000679         2448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42945_length_2448_cov_3.485583, whole genome shotgun sequence.
ACCESSION   DAOSPL010000679 DAOSPL010000000
VERSION     DAOSPL010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42945_length_2448_cov_3.485583"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PLQ54_12305"
     CDS             <1..868
                     /locus_tag="PLQ54_12305"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOS94091.1"
                     /translation="HVVENCVIHSVGRIDRTYTPCIQLEGVGNRVAHNLLYDCPSSAM
                     RIEGNDHQIEYNIFHSVVLESDDQGAIDIFANPSYRGIVYRHNIFHHIGNVAGKAGLH
                     GQAGIRLDDAISGQLIYGNIFYRSANGNFGGVQMNSGRDNVIDNNVFVECRQGVSGGY
                     YPGNSVWSALRETGAYPGVILSELYMQRYPEMARMMEDPGRNFLWGNLFYRCGPVATY
                     PDGLDMFDNQVRDGDPGFAAPEAGDFGLRADAELTHTMGFRPIPVDEIGLYESPLRAS
                     WPVSVEPIAAAR"
     gene            1087..>2448
                     /locus_tag="PLQ54_12310"
     CDS             1087..>2448
                     /locus_tag="PLQ54_12310"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOS94092.1"
                     /translation="MPRAARAGVLACAALALVATIPAHSDAEGRFGGAIDTLESVAVV
                     DHLPAHDAVPLTVEMWVRLRDRDHYNILIANGLKSSPTHWELFTWPGDGCLTAYLPGC
                     QPDHCRTDVPVCDDEWHYVAMVLEAKQVRLYVDGEEASHTPITRDPAAQPVAGPAVIG
                     SLVARELGCNGLIDEVRLSSSARDIVGLPGGPFAADADTIGLWHFDEADSPEGWPDAS
                     GAGRTAARAAAVVTMDRVDVTRMTPEEAAEWDKAHLQLGGPSLAIAPPPVDVPALRAS
                     LAEALADLRLPTLAGAGSTRDAVLADWEEQYYRLDFRLKGQERLPAGAAEQVYDRHAL
                     VFPDDGDPLGVVLRRTRALVDDLGRAGPMVPLGRDLDALAARAARVPVRDATRRKGYY
                     LAACALQRRIALANPLLDFDSILFVARGVYKGSRVTGPTSTADVYGQHFNTQYFGFNS
                     IPGG"
BASE COUNT          388 a          846 c          831 g          383 t
ORIGIN      
        1 ccacgtggtc gagaactgcg tcatccactc cgtgggccgc atcgaccgca cctacacgcc
       61 ctgcatccag ctcgagggcg tgggcaaccg tgtcgcccac aacctgctat acgactgccc
      121 cagcagcgcc atgcgtatcg agggcaatga ccaccagatc gagtacaaca tcttccacag
      181 tgtcgtcctc gagtccgacg accagggcgc catcgacatc ttcgccaacc cgtcctaccg
      241 cgggatcgtc taccggcaca acatcttcca ccacatcggc aatgtggcgg gcaaggccgg
      301 tctccacggc caggcgggca tccgcctcga cgatgccatc agcgggcagc tcatctatgg
      361 caacatcttc taccggagcg ccaacgggaa cttcggcggc gtccagatga acagcggccg
      421 cgacaatgtg atcgacaaca acgtcttcgt cgagtgccgg cagggcgtca gtgggggcta
      481 ctacccgggc aacagtgtct ggagcgcact ccgcgagacg ggcgcctacc cgggtgtgat
      541 cttgagcgag ctctatatgc agcgctatcc tgagatggct cggatgatgg aggacccggg
      601 gcgcaacttc ctgtggggta acctgttcta ccgctgcggg ccggtggcca cctacccgga
      661 cggcctggac atgttcgaca accaggtgcg cgacggcgac cccggcttcg ccgccccgga
      721 ggcgggcgac ttcggcctcc gggcggacgc cgagctcacc cacaccatgg gtttccggcc
      781 gatccccgtg gacgagatcg gcctctacga gagcccgctc cgtgcgagct ggcccgtctc
      841 ggtcgagccc atcgccgcgg cgcgctagcg acgactcccg ccgggacagc ccggtgggca
      901 tcgccaggga tcaccggagc aatgccctgc cgaaagacgg ccgcatcgag ttgcgccggc
      961 atgccgcgcg gctgccccgt tcaccgcggg gcggctgccc ggccgccttg tgtggcgctg
     1021 ggggctgggg tacagtggcc gggaggtcac atcagaggcc gtgtgaggcc cttctggagg
     1081 ttcgagatgc cccgcgctgc ccgggccgga gtcctggcgt gcgccgccct ggcgctggtg
     1141 gccacgatcc cggcccactc ggatgccgaa ggccgtttcg gcggagccat cgacacgctg
     1201 gaatcagtcg ccgtcgtcga ccacttgccg gcgcacgatg cggtgccgct gacggtcgag
     1261 atgtgggtca ggctcaggga ccgcgaccac tacaacatcc tgatcgccaa cggcctgaag
     1321 tcgtctccca cccattggga gttgttcacc tggcccgggg acggctgcct gacggcctat
     1381 ctaccgggct gccaacccga tcactgccgg actgacgtgc ccgtctgcga cgacgagtgg
     1441 cactacgtcg ccatggtgct cgaggcgaag caggtgcggc tgtatgtgga tggcgaggag
     1501 gcctcgcaca caccgatcac gcgcgacccg gcggcgcagc cggtggctgg gcccgccgtc
     1561 atcgggtctc tggtagcgcg ggaactcggg tgtaacgggc tgatcgatga ggtgcggcta
     1621 tcgagttcgg cgcgcgacat cgtcgggcta ccgggcggcc ccttcgcggc cgacgcggac
     1681 acgatagggc tgtggcactt cgacgaggca gacagcccag agggttggcc ggatgcctcg
     1741 ggggccggcc ggacggcggc ccgggccgcc gcagtggtca ccatggaccg ggtcgacgtt
     1801 acgcggatga cccccgaaga ggcggccgag tgggacaagg ctcacctaca actgggtgga
     1861 ccttcgctgg ctatcgcgcc gccaccggtt gatgtgcccg cattgcgggc gtctttggcg
     1921 gaggcactgg ccgacctccg cctgccgacc ctcgccggcg ccggaagcac gcgcgatgcc
     1981 gtgctggccg actgggagga gcagtactac cgcctcgact ttcgattgaa gggccaggag
     2041 agactccccg cgggcgcggc ggagcaggtc tatgaccggc acgcgctggt attcccggac
     2101 gacggcgacc cgctaggcgt tgtactccgc cgcacgcgcg cgctggtcga tgatctgggt
     2161 cgagcgggcc cgatggtacc gctcggcagg gatctcgacg cactggcggc gcgggcggcc
     2221 cgagtgcccg ttcgcgacgc cactcgtcgc aagggttact acctggccgc gtgcgcgctg
     2281 cagcgccgga tcgcgctcgc caacccactg ctcgacttcg actcgatcct cttcgtggcc
     2341 cggggcgtgt acaagggctc gcgcgtgacc gggcccacct ccaccgccga tgtctacggg
     2401 cagcacttca acacacagta ctttggcttc aacagcatcc ccggcggt
//
LOCUS       DAOSPL010000680         2448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42957_length_2448_cov_2.559967, whole genome shotgun sequence.
ACCESSION   DAOSPL010000680 DAOSPL010000000
VERSION     DAOSPL010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42957_length_2448_cov_2.559967"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="PLQ54_12315"
     CDS             complement(<1..1077)
                     /locus_tag="PLQ54_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94093.1"
                     /translation="MGFVLGAVAPASMQSLAEFSPVADDAYYPAEERPERARLGWPGL
                     VAWDGDAGRFELPGANGPVLARPGETAGPWRLIARLSAPGGDVAVLEHNAARWGVFLY
                     LAPGGVRAEVRKSVGSLSHIQPDSVEYPVDYVATLMGCHGDVLGDRVLGSGEPSYDRV
                     AGYLPDLAAYMPISDSRSARKAVVDADGRIGYLAPEYGRDRIRDALFDPCIELGVSGC
                     LAAKRGVLGGHLPVADFAFVGADGSRAWEQIAFAPPEAPDAVYLGLKVDDEWSYRELV
                     SGQTLSSEAFHSALLARWRLAREAGRQGMTVRLPEPRLADAWLAALLQAVSTYEGAHP
                     RYGLGVYAEAAHDGFPPTTIWMVWC"
     gene            1242..2129
                     /locus_tag="PLQ54_12320"
     CDS             1242..2129
                     /locus_tag="PLQ54_12320"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS94094.1"
                     /translation="MRPGECVCLALCVWCAVAGSAAVAQDKSDARDGAEDAFVSLLVN
                     GLILESDAQFHLGDYRKTTRAALSASALDPSYIDGYDSSAWLIWSMGYDANAVKVLKC
                     GIEANPGSAYLRHALGTHYLTTKRHRQAMPWFSQAIAIEPSVMSYMNLGHCYKALHQP
                     RMAVRMYREALRLDPDNGTVKANLEWMHQRMGVMGEGFTQQAGYLSPLRYYQLRLRTE
                     TGHLPSPAEARLAIARAQSKAPLYLTTRQKHLAEDATRPPMTGPPGGMPGPMMGPAGR
                     LQTTSAPDQSADLHLVVQR"
     gene            complement(2093..>2448)
                     /gene="hypB"
                     /locus_tag="PLQ54_12325"
     CDS             complement(2093..>2448)
                     /gene="hypB"
                     /locus_tag="PLQ54_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009505618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOS94095.1"
                     /translation="LPSDRLDLLLIENVGNLVCPAAFDLGEDLKVAVLSVTEGDDKPV
                     KYPKLFREAAVTLISKMDLLPHCGGDVEAIVANLRAVQPAAEIIRTSVRTGEGLDAWA
                     EWLHQRWTTKCRSAD"
BASE COUNT          428 a          868 c          810 g          342 t
ORIGIN      
        1 gcaccagacc atccagatgg tggttggcgg gaagccgtcg tgcgccgcct cggcatagac
       61 gccgaggcca tacctgggat gagcgccctc ataggtcgag accgcctgga gcagggccgc
      121 gagccaggca tcggccagcc ggggctccgg caggcggacg gtcatgccct ggcgaccggc
      181 ctcgcgcgcc agccgccagc gtgccagcag cgccgagtgg aatgcctcgg agctcagtgt
      241 ctgacccgac accagctcgc gatacgacca ctcatcatcc accttcagac cgaggtagac
      301 ggcgtccgga gcctcgggtg gcgcgaaggc gatctgctcc cacgccctgc taccgtcagc
      361 cccaacgaag gcaaagtcgg ccacgggcag atggccgccc agaacgcccc gtttggcggc
      421 cagacagccc gagacaccca gctcgatgca ggggtcgaaa agcgcatcgc ggatccggtc
      481 acggccgtac tcgggcgcca ggtacccgat gcggccgtcg gcatcgacca ccgccttccg
      541 cgcggagcgg gagtcagaga tcggcatgta ggcggccaga tcggggaggt agccggcgac
      601 ccggtcatag gagggctcgc ccgaacccag cacccgatca cccaagacgt ccccgtggca
      661 gcccatgagt gtcgccacat agtccaccgg gtactcgacg gaatcgggct gaatgtgtga
      721 gagcgagccc acggacttcc ggacctcggc ccgaacgccc cccggtgcga ggtagaggaa
      781 caccccccag cgcgcggcgt tgtgctccag cacggccacg tcgccccccg gggctgagag
      841 gcgcgcgatc agacgccacg gtccggcggt ctcgcccggc cgcgccagca cgggcccgtt
      901 ggcaccaggc agctcgaacc ggccagcatc accgtcccag gcgaccaacc cgggccaccc
      961 gagccgcgcg cgctccggac gctcctcggc cgggtagtag gcatcgtcag ccaccgggga
     1021 gaactcggca agtgactgca tcgacgcggg agccacagct cccagcacga atcccatggt
     1081 gggcgtgagc gtggacgacc cctccggctc caagcgagga tgcccaacag tgtagcagct
     1141 cgccgaggtc gccggcgagg ccgaccaggg gatctgtgtg gcgctggcga actacctccc
     1201 aacggcgacc gcggtcgctg gtgggagaag gagggcgtcc gatgaggcct ggtgagtgcg
     1261 tgtgcctggc gctgtgcgtg tggtgtgccg tggcgggatc cgctgccgtg gcccaagaca
     1321 agtccgatgc ccgcgacggt gccgaggacg cgttcgtgag ccttctggtc aatggactga
     1381 tcctggagtc ggatgcccag ttccacttgg gcgactaccg caagaccacc cgcgccgctc
     1441 tgtcagcatc ggcactcgat ccgtcctaca tcgatgggta cgactcctcg gcctggctca
     1501 tctggagcat gggctatgac gcgaacgctg tgaaggtcct caagtgcgga atcgaggcga
     1561 accccgggtc tgcgtacctg cgccatgccc tgggcacgca ctacctcaca acaaagcgcc
     1621 atcgccaggc catgccgtgg ttcagccagg ccatcgccat cgagccatcc gtcatgagct
     1681 acatgaacct cggccactgc tacaaggcgc tgcaccagcc ccggatggcc gtgcggatgt
     1741 acagggaggc gctgcggctc gaccccgata acgggacggt caaggcgaac ctggagtgga
     1801 tgcaccagcg aatgggggtc atgggcgagg ggttcaccca gcaggccggg tatctgtcgc
     1861 ccctgcgcta ctaccagctg aggctccgca cggagacggg gcatctgccc tcgcccgccg
     1921 aggcccggct ggccatcgcc cgcgcccagt ccaaggcgcc gctgtacctg acaacccggc
     1981 agaagcacct cgccgaggat gccactcgcc ctccgatgac cggcccgccc ggcggcatgc
     2041 ccgggcccat gatgggcccc gcgggccggc tgcagaccac atcagcaccc gatcagtccg
     2101 cggatctgca tttggtggtc cagcgctgat gtagccactc ggcccatgcg tcgagtccct
     2161 cgcccgtccg cacggacgtg cggatgatct cggccgccgg ctgcaccgcc cgcaggttcg
     2221 cgacaatggc ctccacatcg ccgccgcagt ggggcaggag gtccatcttg ctgatgaggg
     2281 tcacggccgc ttcgcggaac agcttcgggt acttcaccgg cttatcatca ccctcggtca
     2341 cgctgagcac agccaccttc aggtcctcgc ccagatcgaa agcggcggga cacaccaggt
     2401 tgcccacgtt ctcgatcagc agcaggtcga gccggtcgct tgggaggc
//
LOCUS       DAOSPL010000681         2446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_42990_length_2446_cov_2.772062, whole genome shotgun sequence.
ACCESSION   DAOSPL010000681 DAOSPL010000000
VERSION     DAOSPL010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42990_length_2446_cov_2.772062"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="PLQ54_12330"
     CDS             complement(<1..1230)
                     /locus_tag="PLQ54_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94096.1"
                     /translation="MKATWATSMVVVVAIGGGSAGLAQQYALVVVPPMAEGASRVVTA
                     RADVSDIARATGADGLLASAGEVPACRAAVERPDADPLPIPCQGNVDPDGQFVVSLWL
                     DPSAAERRVRVYPEGAMVHQPQCPAALQFDQSADRVDVVGAGFRVSHAREKAWLPARI
                     EFAGSGKVFDSFALNDRIYHPERGGYALRDDAAAKLEVLAAGPLRVALRVSARYVRDG
                     EPAPGGAQATYTFEYLAGSPELRVEARVTQDAELQWTEIHLLEINFPDESFMRCALGD
                     PEEERELTASSEAIRGSRWGALLDGMNVLALVGGPCTVYDGRGGYGTYLHGPWTDFRS
                     REWRGSATVWIGSGPTALAEARQAARSPAVPRPIRLSTPRLEGAVAGMERTAREAQDV
                     SEAGRAAWHAALVRQLVP"
     gene            1593..>2446
                     /locus_tag="PLQ54_12335"
     CDS             1593..>2446
                     /locus_tag="PLQ54_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS94097.1"
                     /translation="MIRVEGLTKYYGPYPAVRDVSFHVREGEIVAFLGPNAAGKTTTM
                     RVLTCYHPPSFGEAFIAGHSVRTEALEARQQIGYLPESIAMYEEMSVVSYLRFCSRLR
                     DVPRHEVEDRVESAMTRCRVDDVADRAIGKLSKGYRQRVGLAQALVHDPPVLILDEPT
                     IGLDPAQIIETRELIRRLSGERTVMLSTHILPEAQALAQRVIIINHGRIVAEDTPEAL
                     TGRVQKVDTLFIRISETSDAVAQALRVIPEVKNIRVAEREKNSVAYHVDTARGHDIRA
                     DLARFIVN"
BASE COUNT          410 a          872 c          792 g          372 t
ORIGIN      
        1 cgggacgagt tgcctcacga gcgccgcgtg ccaggcggcg cggcctgcct ctgatacatc
       61 ttgcgcttct ctcgcggtcc gctccatgcc ggcaacggcc ccctccagtc tgggcgtcga
      121 gagccgaatg ggccgcggca ccgcagggct ccgggcggcc tgccgtgcct cagcgagagc
      181 agtcgggccc gagccaatcc acaccgtggc cgaccctctc cactcccgcg aacggaagtc
      241 agtccacggg ccgtgtaggt aggtgccgta acccccgcgg ccgtcataga cagtgcaggg
      301 cccgccgacc agagccagca cgttcatgcc atcgagcagc gcgccccacc gcgagccgcg
      361 gatagcctcc gaggaagccg tcagctcgcg ctcctcctcc gggtcgccga gtgcgcaccg
      421 catgaagctc tcgtcgggga agttgatctc gagaagatgg atctcggtcc actggagctc
      481 cgcatcctgc gtcacgcggg cctcgacccg cagttccggg ctgccggcca gatactcgaa
      541 ggtgtatgtc gcctgcgccc cgccgggagc gggctcgccg tcgcgcacat agcgggcgct
      601 cactctcagg gcgacgcgga gtggccccgc ggcgagcacc tcgagcttcg cggcggcatc
      661 gtcgcgcaga gcgtacccac cccgctccgg gtggtagatc cggtcgttga gcgcgaacga
      721 gtcgaagacc ttccctgagc cggcaaactc gatccgggcc ggcaaccagg ccttctcgcg
      781 agcgtgcgat acgcggaacc ccgcgccaac gacatcgacg cgatcagcgg actgatcgaa
      841 ctggagagcc gccgggcact ggggctggtg gaccattgcg ccctcagggt acacccgaac
      901 gcgcctctcg gccgcgctag ggtccagcca cagggagaca acgaactgcc cgtcggggtc
      961 cacgttgcct tggcacggga tgggcagagg gtcggcgtcc gggcgttcga cagcggcgcg
     1021 acaggccgga acctcgccgg ccgaggccag caggccgtcc gcccccgtcg cccgcgcgat
     1081 gtccgagaca tccgcccggg ccgtgaccac tcgcgaggcc ccctcggcca tgggtggaac
     1141 cacgacgaga gcatactgct gtgccaaccc ggccgaaccg cctccgatcg ccaccacaac
     1201 caccatggag gtcgcccatg tcgccttcat tgacttacca ccactcctcc gcgggccgct
     1261 agtccctgat tccggcgctt cggggagttc gcgggcctcc cctgcctgcg ggggatccga
     1321 gaccgcatgt tggggtacga tctcgtgatc gggattcggg atcgtcgggg cctggccggc
     1381 cacccggtct ccttgacccg cccggaagga gctgtgtata ctctcgcgct agcacactcg
     1441 gcccgagagt gctagcgcgc tgcctaccat tcggtctggc aggctgggtg tcaaccgcca
     1501 acccctatgg cggaatgaac gtcggagcat ctcagtgggt cggcctcgcg cgcgccgcgc
     1561 ggggtttccg tacgtgagat ggagggacgt gcatgattcg agtggagggg ctgacgaagt
     1621 actacggccc ttatcccgcc gtccgcgacg tcagcttcca cgtccgagag ggcgagatcg
     1681 tcgccttcct tggcccaaac gccgcgggca agacgaccac catgcgtgtg ctaacgtgct
     1741 accacccccc gagctttggc gaagccttca ttgcggggca cagcgtgcgg accgaggcac
     1801 ttgaggcccg ccagcagatc ggctacctgc ccgagtccat tgccatgtac gaggagatgt
     1861 cggtcgtctc gtacctgcgc ttctgctcgc ggctccgcga tgtgccacgg cacgaggtcg
     1921 aggaccgcgt cgagtccgcc atgactcggt gccgcgtcga tgatgtggcc gatcgcgcca
     1981 tcggcaagct ctcgaagggc taccggcagc gcgtcggcct cgcccaggcg ctggtccacg
     2041 atccccccgt cctgatcctg gacgagccca ccatcggcct ggaccccgcg cagatcatcg
     2101 aaacgcgtga actgatccgc cggctgagcg gtgagcgcac cgtcatgctc tcgacacaca
     2161 tcctgcccga ggcccaggcg ctggcgcaac gggtcatcat catcaaccac gggcgcattg
     2221 tggccgagga cacgcccgaa gcgctcacgg gccgagttca gaaggtagac acgctcttca
     2281 tccgcatatc cgagaccagc gacgccgtgg cacaggcgct ccgggtcatt cccgaagtga
     2341 aaaacatacg tgtggcggag cgtgagaaga acagcgtcgc ctaccacgtc gacacggcgc
     2401 gcggacacga catccgggcg gacctcgcca ggttcatcgt gaacaa
//
LOCUS       DAOSPL010000682         2444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43035_length_2444_cov_2.734617, whole genome shotgun sequence.
ACCESSION   DAOSPL010000682 DAOSPL010000000
VERSION     DAOSPL010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43035_length_2444_cov_2.734617"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..670
                     /locus_tag="PLQ54_12340"
     CDS             149..670
                     /locus_tag="PLQ54_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94098.1"
                     /translation="MLLRIALGLYAIALCAWEPGLGAAFPVLGARPHLDLVIITSIGL
                     LLGQEDGAVAGFLCGMLEVAHTAGPGGGAFVASRVLVGYAAGSASHSLFARSLLVPVS
                     CTAVATVGAEVVRFILAPYPDLSTWARLVLVQSIYNGALAVAIHPVAAWLSRRLPSRP
                     RAPRQAINRPRAI"
     gene            complement(648..2234)
                     /locus_tag="PLQ54_12345"
     CDS             complement(648..2234)
                     /locus_tag="PLQ54_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94099.1"
                     /translation="MAPGHLALYLLTFCWGVARAVPGPTGIPDDWLTVASLIGECAVG
                     PTERIGLDTDGDGDPDRVAYRDGHALVVAVDADDDARTAADVPDADGDVLAIDLQDDG
                     RIDRILDRFDDDGDGDADREHHYYIGRSWLGEPAGLVAAWDLNDNNRLWALHGYSYDQ
                     GSCQWDSDFGAGGAEGFCLFGRDAAAGEWVPRFECPFYFFDPDHDHHPEAALRLEGAG
                     ARMQFLRLSFDADDDAGREAPYDYDVGLMAVGPVDLPDSWLAPEPLRWGFTSPFLQYS
                     GAELRAPTLPWSSALLVWDENDRNIDPGDPGAHERWEGVINAPYTGFPQVGGPSCGRI
                     NKRYELDADNSGGLRLYPSWVDCRLHLLGAEYGELLMDADDDGSPDWSAGYRDTDRDG
                     FFDQWTLTRAGGGEAECTIAAPPPYMLDAGGHPARTASPIAPRWGELRQAYGPLLAQG
                     TMRARRVAHALGLGDSLAAGPSERGRYGAEGQIRGALARAIDEAVAARDAARAVRLSR
                     ARECWEAGLVESAVRWLGAG"
BASE COUNT          360 a          876 c          843 g          365 t
ORIGIN      
        1 cgggtcgaca cctagcacca gatcgcggtg ctggacccgg cgacgggcac gcggcggcct
       61 cgagctcgag ccacggtgcg gcccctcacg gacgccctct cgctcagttc ggtctgggtg
      121 atccggctcg gcgcgggagg cgattgaggt gctcctgcgg atcgcgctgg ggctgtacgc
      181 catcgccttg tgtgcgtggg aaccgggcct gggggcggcc ttcccggttc tgggcgcccg
      241 cccccatctt gacctcgtca tcatcacatc cattgggctt ctgctgggtc aggaagacgg
      301 cgcggtcgcg ggcttcctgt gtgggatgct cgaggtggcg cataccgcgg ggccgggggg
      361 aggcgccttc gtggcctcgc gagtactcgt cggttacgcc gccggcagtg cgagccacag
      421 cctcttcgcc cgcagcctcc tggtccccgt ttcatgcacg gcggtggcca ccgtcggggc
      481 cgaggtcgta cgcttcatcc tggcgcctta cccggacctc tcgacttggg cacggcttgt
      541 gctggtgcag tcgatctaca acggcgcact ggcagtggcc atccatccgg tggccgcgtg
      601 gctgtcgcgg cgcctgccct cgcggccgcg cgccccccgc caggccatca accggccccg
      661 agccatctga cggcggactc caccaggccc gcctcccagc actcacgggc gcgagaaagc
      721 cggaccgcac gcgcggcgtc tcgggcggca accgcctcgt cgatcgcgcg ggccagcgcg
      781 ccgcggatct gtccctcggc cccatagcgg ccgcgctcgc tcggcccggc ggccagggag
      841 tccccgaggc cgagcgcatg ggccacgcgc cgggcccgca tggtcccttg cgccaggagc
      901 ggaccgtagg cctgacgcag ctccccccag cgaggcgcta tgggcgaagc tgtccgggcc
      961 ggatgaccgc cggcatcgag catgtagggc ggcggtgcgg cgatggtgca ctccgcctcc
     1021 ccaccaccgg cgcgggtcag ggtccactgg tcgaagaagc cgtcgcgatc ggtgtcgcgg
     1081 taccccgcac tccagtccgg cgagccgtcg tcgtccgcgt ccatcagcaa ctcgccatac
     1141 tccgcgccga ggagatggag ccgacagtcg acccacgagg ggtagaggcg caggccgccc
     1201 gagttgtcgg catcgagttc ataccgcttg ttgatgcggc cacacgatgg cccaccaacc
     1261 tgcgggaaac ctgtataggg cgcgttgata acaccctccc agcgctcatg cgcaccgggg
     1321 tcaccgggat cgatgttccg gtcgttctca tcccaaacca gaagcgccga tgaccaaggt
     1381 agcgtggggg cgcgcaactc ggcgccgcta tactggagaa aggggctcgt gaatccccaa
     1441 cgcagcggct ccggcgccag ccaggagtcc ggcaggtcca cagggccgac agccatcaga
     1501 cccacatcgt agtcgtacgg cgcctcgcgc cccgcgtcgt cgtcggcgtc gaagctcagc
     1561 cgcaggaact gcatgcgggc accggccccc tcgagccgca gtgcggcctc gggatggtgg
     1621 tcatggtccg ggtcgaagaa gtagaaggga cattcgaagc gggggaccca ctccccggca
     1681 gctgcatcgc gcccaaacaa gcagaagccc tcggctccac ccgcgccgaa gtcgctgtcc
     1741 cactggcagc tcccctggtc gtagctgtag ccatggaggg cccacagccg gttgttgtcg
     1801 ttcaggtccc acgcggccac cagacctgcg ggctccccga gccagctgcg tccgatgtag
     1861 tagtggtgct cgcggtcggc gtcaccgtca ccatcatcgt cgaagcggtc gaggatccgg
     1921 tcgatgcggc cgtcgtcctg gagatcgatg gcgagcacgt cgccgtccgc gtcggggacg
     1981 tcagcggctg ttcgggcgtc atcgtcggca tccacggcca cgaccagggc gtggccatcc
     2041 cggtacgcga cgcggtcggg gtcgccgtcg ccatcggtgt ccagcccaat gcgctccgtg
     2101 gggccgacgg cgcactcgcc aatcagcgag gccacggtga gccagtcgtc gggaatgccc
     2161 gtcgggcccg gaaccgcccg ggccactccc cagcagaatg tgagcaggta taaggcaaga
     2221 tgccctgggg ccacggccgt gcctcctgtc aagcgctgtc aagcgcgtcg gcgagcagtc
     2281 cccccggttc gccggcccct ctccagggcc tgctcgacgc atgaccggag gtccctggca
     2341 ccggtcccgc ttgggcgcag accaaccggg ggtgtcccgc cgaaaacgcg gcccgtgacg
     2401 agatgcgtcg gctggccgac gaggtgtgag agtcagccga gaga
//
LOCUS       DAOSPL010000683         2444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43036_length_2444_cov_2.734198, whole genome shotgun sequence.
ACCESSION   DAOSPL010000683 DAOSPL010000000
VERSION     DAOSPL010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43036_length_2444_cov_2.734198"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2119)
                     /locus_tag="PLQ54_12350"
     CDS             complement(<1..2119)
                     /locus_tag="PLQ54_12350"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HOS94100.1"
                     /translation="MLNLNGTWKLKDFDPGQGLAQGAQRPETDTATWIDVAVPGDVHR
                     ALVASGRLAEPFDDQNIEACEWVERREWWYRTVFTSPVTAGGERIVAEFDGLDTFATV
                     YVNGQEVGRSANMLVESEFDVTDALHPGAANTLAICFSPTVATIEQRDNSRFWSAFYT
                     PRVWVRKAQMNFGWDWGPRLVTVGIWRGARLTRRGPARIESHYVRTHAIDEEGAVVLV
                     GARVEDREPAAEPLGLSVSLSDGDRTFSGYAEVSGNRAEVRLVVPQPRLWWSNGLGEP
                     FRHDVRIDLVQEGAVADSRADKVGLRTIRLLQEPDGDGLGSGFTFVLNGRKVFCKGAD
                     WIPVDSFIGSAPPERYRALVRLARDAHMNMLRVWGGGVYEHDAFYEACDDMGILVWQD
                     FMFSCASYPDFDEAFVAEVAREAELVVKRLRNRACVALWCGNNENQWIDDMCHTHDPQ
                     VPLFGQRLYDEVLPGVVARLDPTRPYWPGSPYGGNDHNSEREGDRHNWQVWAGQVYPR
                     RFGEPAEGNNTPEGVSFRHYAEDECRFCSEFGIHASPMLRTLQRHVKDGVLEYDSPAF
                     LYRIKDPDGTRKVRMMEAHIGQPRDLDEYATYSMLVQAEGLKFGIEHYRRRMFLCGGA
                     LLWQLNDCWPGISWSVIDYYLNPKAGYYYVKRACAPVLVSFQPESDGVSVWLTNDTWK
                     PVETRIRLTVRRVGDATPVRQIIL"
     gene            complement(2191..>2444)
                     /locus_tag="PLQ54_12355"
     CDS             complement(2191..>2444)
                     /locus_tag="PLQ54_12355"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOS94101.1"
                     /translation="SFSCGTKMAMVARGEADFYLSSAHAYSDWDLCAGHLLVEEAGGT
                     VSDGQGGALIYGGASVTKDAGIIATNGRLHTAALQMLRR"
BASE COUNT          395 a          852 c          780 g          417 t
ORIGIN      
        1 ccaggatgat ctggcgcacg ggggtcgcat cgccgactcg ccggactgtc agccggatcc
       61 gggtctcgac cggcttccag gtgtcgttcg tcaaccacac cgacactcca tcgctctcgg
      121 gctggaacga gacgagcacg ggcgcacagg ctctcttgac gtagtagtag ccggctttgg
      181 ggttgaggta gtagtcgatg acactccacg agatccccgg ccagcagtcg ttgagctgcc
      241 agagcagggc gccgccgcac aggaacatcc gcctgcggta gtgctcgatg ccgaacttca
      301 gcccttcggc ctgcacgagc atcgagtacg tcgcgtactc atccaggtcg cgcggctgcc
      361 cgatgtgcgc ctccatcatc cgcaccttgc gcgtcccgtc cgggtccttg atgcggtaca
      421 ggaacgccgg cgaatcgtac tcgagaacac cgtccttgac gtgacgctgc agcgttcgca
      481 gcatgggcga ggcgtggatg ccgaactcgc tgcagaaccg gcactcgtcc tcggcgtagt
      541 ggcggaagga gacaccctcg ggcgtgttgt tgccttccgc tggctccccg aagcgcctcg
      601 ggtagacctg gcctgcccat acttgccagt tgtgccggtc cccctcgcgc tcggagttgt
      661 ggtcattgcc gccatagggg ctccccggcc agtagggtcg cgtgggatcg aggcgcgcca
      721 cgacaccggg cagcacctcg tcgtagagac gctgaccgaa gaggggtacc tgaggatcgt
      781 gggtgtgaca catgtcgtcg atccactggt tctcgttgtt gccgcaccac agggcgacgc
      841 aagcccggtt gcgcaagcgc ttcacgacga gctcggcctc ccgggctact tcggcaacga
      901 atgcctcgtc gaaatcgggg taagacgcgc aactgaacat gaagtcctgc cacacgagga
      961 tgcccatgtc gtcgcaggcc tcgtagaacg cgtcgtgctc atagacgcca ccgccccaca
     1021 cgcgcagcat gttcatgtgg gcgtcgcgag ccagccgcac cagggctcga tagcgctccg
     1081 gaggtgccga gccgatgaag gagtccaccg ggatccagtc ggcccccttg cagaacacct
     1141 tccgcccgtt gaggacgaat gtgaagccgc tgccgaggcc gtcgccgtcc ggctcctgta
     1201 gcaggcggat cgtgcgtagc cccaccttgt ccgcccgcga gtcggccacg gctccctcct
     1261 gcacgagatc gatgcgcacg tcgtgccgga acggctcgcc aagtccgttg gaccaccaca
     1321 gtctcggctg cgggaccacc aggcggactt ccgcgcggtt cccggagacc tccgcatagc
     1381 cggagaaggt gcggtcgccg tccgagagtg acacgctcag gcccagcggt tccgctgcgg
     1441 gctcgcgatc ctccacccgg gcaccgacca gcaccacggc accctcttcg tcgatggcat
     1501 gggtgcgcac gtagtgtgat tcgatccgcg cggggccacg ccgtgtgagc cgcgcccctc
     1561 gccagatccc gaccgtcacc agccgggggc cccagtccca gccgaagttc atctgcgcct
     1621 tccggaccca cacccgcggc gtgtagaaag cggaccagaa ccgggagttg tctcgctgct
     1681 cgatggtggc gaccgtgggg ctgaagcaga tcgcgagcgt gttcgcggcg cccggatgca
     1741 gcgcatcggt gacatcaaac tcgctctcga ccagcatgtt ggcgctccgt cccacctcct
     1801 ggccattgac atagacggtg gcgaaggtgt ccagcccgtc gaactccgcg acgatgcgct
     1861 cgcccccggc ggtaacgggc gatgtgaaca ccgtgcggta ccaccactcg cgccgctcga
     1921 cccattcgca tgcctcgatg ttctggtcat cgaacggctc ggcgagtctt cctgaagcca
     1981 ccagggcgcg gtgcacgtcg ccgggcaccg ccacatcgat ccacgtcgcc gtatcggtct
     2041 cgggccgctg ggcgccctgg gccagtccct gccccgggtc gaagtccttg agtttccatg
     2101 tgccattcag gttcagcatc gcgcggttct ccgctctgtg tctgggggag gccagactcc
     2161 ccccggcgtc acccgtcacg ccacgccgtg ctaacgcctc agcatttgga gggcggccgt
     2221 gtggagccgg ccgttcgtgg cgatgatgcc ggcgtccttg gtcaccgacg ctccgccgta
     2281 gatgagggca ccgccttggc cgtccgatac ggtgcctccg gcttcctcca ccagcaggtg
     2341 cccagcgcac aggtcccagt ccgagtaggc gtgggccgaa gacaggtaga agtccgcctc
     2401 gccgcgcgcc accatggcca tcttcgtgcc gcaggagaag ctga
//
LOCUS       DAOSPL010000684         2440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43131_length_2440_cov_3.084696, whole genome shotgun sequence.
ACCESSION   DAOSPL010000684 DAOSPL010000000
VERSION     DAOSPL010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43131_length_2440_cov_3.084696"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..2071
                     /locus_tag="PLQ54_12360"
     CDS             59..2071
                     /locus_tag="PLQ54_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94102.1"
                     /translation="MAVLLWLGVLTGACACGRQIRTWLRATGEARCLEVGYDAALGLG
                     VLAYLVLGLGLVGLLYPWALALAALSLYAIGWRQIPERVRWLREARWPAGRLESLLWA
                     ALIACGVAALVRNLVPPGGDEWDTLVYHLTGPKQFLQAHRIHFIEHDHHTNFPFNMEM
                     LYLLGLGLHSDRLAKLFHLAMYLLCVLALCGAGHSLGRPGNGAGVPGAVGPAGALAFA
                     TIPAVAWEAGTAYIDLGLALYGLLTVLAWSEWRQGRGRGWLVAAGLFAGLALGVKMTG
                     LLVVLFVCGATLLTEAWRRQTRGAVASTLLLGATAIGLASPWYIKTYVYTGNPVFPFF
                     YEVLGGRGWSREHARTYQEEQQSHGTHAIARAVAEGTYRRMEVGVGAQSETADASASA
                     AVVRALFRRGDWPNAAAALVVPLLEVIDPARFWEQAAVPCIIGPLFVALLPVLIWLRP
                     LPPTLKALLAYAGFGLFAQTHLSQLSRYQIPFFAAGALGVGYGVVHLGRLGRAPRAAG
                     WLSFGTVSACLLALHLLRATAALPTALGVWTAEEVDAATFESHGAFEYLNAHAGPKAK
                     VAVFGEPRLYRLDVPAEFADRFHNTLYPYERMRTPDDFLSALRARGITHILVNPRYHP
                     GVMGPGPKDRVARFIDALAAEGRLEVAFGDPAEQRATFVLRLTGRE"
     gene            2077..>2440
                     /locus_tag="PLQ54_12365"
     CDS             2077..>2440
                     /locus_tag="PLQ54_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94103.1"
                     /translation="MPKAKRRSRKPAVSVPEVSQPESLEWVAPPPPTLAVRRVLWAIL
                     GAFALLSVVCAFVTRMGTAPDEAHHVAYVRYLATEMRLPILSEDSPAGYEAHQPPAYY
                     ALGALVWWVSRGNEYALRL"
BASE COUNT          289 a          798 c          906 g          447 t
ORIGIN      
        1 cgcgaggcgg acgcggccgg gaccgtggtc cggctcctct cggggggcga ctaggccggt
       61 ggctgtgctc ctctggctcg gggtcttgac cggcgcgtgt gcgtgcggcc ggcagatccg
      121 cacctggctg agagccacgg gcgaggcgcg gtgcctggag gtcggctatg atgccgccct
      181 ggggctcggg gtgctcgcgt acctggtgct ggggctgggg ttggtgggcc tgctctatcc
      241 gtgggccctg gctctggcgg cgctgtcgct gtacgccatc ggctggcgtc agatcccgga
      301 acgggtgcgc tggcttcggg aggctcgttg gcccgcgggg cggcttgaga gcctgctttg
      361 ggcggcgctg atcgcgtgcg gggtggcggc cctggtccgg aacctggtgc cccccggtgg
      421 cgatgagtgg gacaccttgg tctatcacct caccggcccc aagcagttcc tgcaggcgca
      481 tcggatccac ttcatcgagc acgatcacca cacgaacttc ccgttcaaca tggagatgct
      541 ttacctgctg gggctaggtc tgcactccga tcgcctggcg aagctgtttc atctcgccat
      601 gtatctgctg tgtgttctgg ccctctgcgg cgctggtcat tcgctggggc ggcccgggaa
      661 tggcgcaggc gtaccgggag cggtgggccc ggccggcgcc ctggccttcg cgaccatccc
      721 cgccgtggcg tgggaggccg gcacggcgta catcgacctg gggctggcgc tgtatggact
      781 cctgaccgtg ctggcgtggt cggagtggcg tcaggggcgc ggccgagggt ggctggtggc
      841 ggccggactg ttcgcgggtc tggccctggg tgtgaagatg acgggcctcc tggttgtgct
      901 gttcgtctgc ggcgcaacac tgctcaccga ggcctggcgg cgccagacac gcggggccgt
      961 ggcgtccaca ctgctgctcg gggccacggc catcggtctg gcgagcccgt ggtacatcaa
     1021 gacgtatgtc tacacgggca acccggtctt ccctttcttt tacgaggtgc ttggtggtag
     1081 agggtggagc cgggagcacg cgcgcaccta ccaggaggag cagcagagcc acggaaccca
     1141 cgccatcgcg agggcggtgg ccgagggcac ctaccgccgg atggaggttg gcgtcggcgc
     1201 ccagtccgag acggcggacg cgtccgcctc ggccgcggtg gtgcgggcgc tgttccgcag
     1261 gggcgactgg cccaatgccg ccgccgccct cgttgtgccg ctgctcgagg tgatcgatcc
     1321 cgcgcggttc tgggagcaag cggcggtgcc gtgcatcatc gggccgctgt tcgtggcgct
     1381 gctcccggtg ctgatctggc tgcgcccgct gccgccgact ctcaaggcgc tgctcgccta
     1441 cgcgggcttc gggctgttcg cgcagacgca tttgagccag ctctcgcgct accagattcc
     1501 gttcttcgcc gcgggcgcgc tgggcgtagg ctatggggtg gtgcatctcg ggcggctggg
     1561 gcgggctcca cgagcggcgg gctggctctc gttcggtacg gtctccgcat gcctcctggc
     1621 gctacacctg ctgcgtgcca cggccgcgct gcccacggcg ctgggagtgt ggacggctga
     1681 ggaggtcgac gccgccactt tcgagtcgca cggggctttc gagtacctca acgcccatgc
     1741 gggcccgaaa gccaaggttg cggtcttcgg cgagccacgg ctgtaccgcc tcgatgtgcc
     1801 ggccgagttc gccgaccggt tccacaacac gctctacccg tatgagcgca tgcgcacgcc
     1861 tgacgacttt ctatcggcct tgcgggcgcg ggggatcacg catattctcg tgaacccgag
     1921 gtaccacccc ggggtgatgg gccccgggcc gaaggaccgc gtggcgcggt tcattgatgc
     1981 gctggcggcc gaaggccggc tcgaggtcgc ctttggcgat cccgcggaac agcgagccac
     2041 ctttgtgttg cgactgacgg gacgggagtg agcggcatgc cgaaggccaa gcgccgctcg
     2101 cgcaagccag cggtttccgt tccggaggtt tcgcagcccg agtctctcga gtgggtggct
     2161 ccgccgccgc ccacactggc ggtgcggcgc gtgctctggg cgatcctcgg ggccttcgca
     2221 ctcctctcgg tggtgtgtgc cttcgtcacc cgcatgggga cggctccgga tgaggcacac
     2281 cacgtcgcat acgtgcgcta cctggcgacc gagatgcggc tgcccatcct ctcggaagac
     2341 tcgcccgccg gttacgaggc gcaccagccg ccggcctatt acgccctggg cgcgctcgtg
     2401 tggtgggtct cgcgggggaa cgagtatgcc ctgcggcttc
//
LOCUS       DAOSPL010000685         2438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43162_length_2438_cov_3.990348, whole genome shotgun sequence.
ACCESSION   DAOSPL010000685 DAOSPL010000000
VERSION     DAOSPL010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43162_length_2438_cov_3.990348"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PLQ54_12370"
     CDS             complement(<1..532)
                     /locus_tag="PLQ54_12370"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOS94104.1"
                     /translation="MGLEMMVAMGISLVGAGPAVYNVADFGAQGQETVADTAAFGEAV
                     KACVAAGGGTVYVPPGRYTLGRVTLGGRVTLHLEAGAEIHPSTDPAHFPPLEGSAESA
                     YKPAYGDNAINCRYAVFYALGARDVTLEGRGCIVGDGKSFWDVKNTGDFAKWNMVAPW
                     FYYGARPFRPIIVMLEE"
     gene            complement(1000..1356)
                     /locus_tag="PLQ54_12375"
     CDS             complement(1000..1356)
                     /locus_tag="PLQ54_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94105.1"
                     /translation="MSASPAGPFSASYMALTGGTASFYFDATNPDNARSALVPRFGGW
                     NPVYTTAVGGVYGSRGSRLMRMLDEGHHGVRLPPDDRERLSIWIDANALFYGTYDPAE
                     QQRQLAGELIAMPELQ"
     gene            complement(1353..1997)
                     /locus_tag="PLQ54_12380"
     CDS             complement(1353..1997)
                     /locus_tag="PLQ54_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94106.1"
                     /translation="MTDIEILRPLVDRTLERAALPCQSERAELLARNNALLPVPRAPV
                     IVSFELIPAEHWHLMLGEDHLKCQAEDARALEWRLRRDLWADEHIPHDHPLWPVAWVT
                     ADYRQVVGWGVDLGRHAVGGGLTAWGYDPPFRDGIEVSRLTMPEFEHHEAGTRRGPPR
                     HRCPCRKRLGANPQRSGPGRTVRRPSRSHGSSSRCWTGIVGRATRLAAVSPAST"
     gene            2082..>2438
                     /locus_tag="PLQ54_12385"
     CDS             2082..>2438
                     /locus_tag="PLQ54_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOS94107.1"
                     /translation="MADLRFRQIHLDFHTSEQIPGIGSEFDPDEFADTLKAAHVNSVT
                     CFSRCHHGWIYHDTARFPERRHPHLTRNLLKEQIDACHQRDIRVPIYITVMWDHFTAR
                     RHPEWIAIDETGRQIGT"
BASE COUNT          426 a          892 c          766 g          354 t
ORIGIN      
        1 actcctcgag catcacgatg atgggtcgaa agggccgggc gccgtagtag aaccatggag
       61 ccaccatgtt ccacttggcg aagtcgccgg tgttcttcac gtcccagaac gacttgccat
      121 caccgacgat gcagccgcgg ccctcgaggg tcacatcgcg cgcccccagc gcgtagaaca
      181 ccgcgtagcg gcagttgatg gcgttgtcgc cataggctgg tttgtaggcc gactcagcgg
      241 agccctcgag gggcgggaag tgggctgggt ctgtcgatgg gtggatctcg gctccggcct
      301 ccaggtgcag agtcacgcga ccgcccaggg tgacgcggcc cagcgtgtag cgcccgggcg
      361 gcacgtagac cgtcccccca ccggcggcaa cgcaagcctt cacggcttcg ccgaaggccg
      421 cggtgtccgc gacggtctcc tgcccctgtg cgccgaagtc cgccacgttg tagacggccg
      481 gtcccgcgcc tacgagggag atgcccatag ccaccatcat ctccaagccc atgccgtgcc
      541 tcctgatcgt cggtgatctc cagcgagggc ctttcccgct cgcggcagcc aagccctgcg
      601 tcggacgcct ccgcaggtag cgacggccgg ccggccgacc aatggtctcc accgctacca
      661 acacgcccgg tcggaggccc gacttcagag cctgtcggag aagtccgcca gagggcctct
      721 gcgaacggaa tgacacgcgc cagcagtccc tcagacgcga ttccaggcac cgggggactg
      781 tccgatgacg ccccaagacc tggcctgaat ggccggaatc ggggtctccc gacaggctct
      841 tcagccgggc gcggcagacc ggcaccctcg tgccgttgtg attgcccctc ccgggagccc
      901 cggcttcagt cggctgggat atggggcggg agccccgcgg tcttggcgaa acagggagcg
      961 ccccctgagc ctgccgaacg gctgcgcgct gccccccgcc tactgcagct caggcatcgc
     1021 gatcagctcg cccgcaagct ggcgctgctg ctctgccggg tcgtaggtcc cgtagaatag
     1081 cgcgttcgcg tcgatccaga tggagagccg ctcccggtcg tcgggcggca gtctcacgcc
     1141 atggtgcccc tcgtctagca tccgcatcag ccggctgccg cgggagccat agaccccccc
     1201 cacggctgtc gtgtagacgg ggttccaccc tccgaatctc ggcaccagcg cactccgggc
     1261 gttgtccggg ttcgtggcgt cgaagtagaa cgacgccgtg ccgcccgtca gcgccatgta
     1321 ggaagccgag aatggccccg cgggcgacgc gctcaagtcg acgccggact caccgccgcc
     1381 agccgggtgg cacgacccac aatgccggtc cagcaccggc tggacgagcc gtgcgaacga
     1441 gaagggcgcc gtaccgtccg gcccgggccg gatcgctgag ggttcgcgcc gagccgcttg
     1501 cggcatgggc accggtggcg cggtggtccg cgccgcgtgc ccgcctcgtg gtgctcgaac
     1561 tccggcatgg tgagacgcga tacctcaata ccgtcacgaa aaggcggatc atagccccat
     1621 gccgtcagcc cgccgcccac ggcgtgccga cccagatcaa caccccagcc caccacttgg
     1681 cggtagtcag ccgtcaccca ggcgaccggc cacaacggat ggtcgtgggg gatgtgctcg
     1741 tcggcccaca ggtcgcggcg cagccgccac tcgagagcac gcgcgtcctc cgcctggcac
     1801 ttcaaatggt cctcaccgag catcagatgc cagtgctcgg cggggatcag ctcgaacgac
     1861 acgatcaccg gtgctcgcgg aacgggcagc aaggcgttgt tccgcgccag aagctcggcc
     1921 cgctcgctct ggcatggcag ggcggcccgc tcgagcgtcc gatcgaccag aggacgcagg
     1981 atctcgatat ccgtcatgac cttcgccgtc ctccaaggac aggcccgcca cagcaacgaa
     2041 gcaccaaacg cacccacccg aacggtcgcg aggagaaagc catggcagac ctcaggttcc
     2101 ggcagatcca cctggacttc cacaccagcg agcagatccc tggaatcggc agcgaattcg
     2161 accccgacga gttcgccgac acgctcaaag ccgcccacgt caactccgtg acatgcttct
     2221 cccgctgcca ccacgggtgg atctaccacg acaccgcacg gtttcccgag cgccggcatc
     2281 cccatctcac gcgcaaccta ctcaaggagc agatcgacgc ctgccatcag cgcgacatcc
     2341 gtgtgccgat ctacatcacc gtcatgtggg accacttcac cgcccgccgg cacccggagt
     2401 ggatcgccat cgacgagacc ggccgacaga tcggcacc
//
LOCUS       DAOSPL010000686         2436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43193_length_2436_cov_4.313734, whole genome shotgun sequence.
ACCESSION   DAOSPL010000686 DAOSPL010000000
VERSION     DAOSPL010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43193_length_2436_cov_4.313734"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..524)
                     /gene="rplM"
                     /locus_tag="PLQ54_12390"
     CDS             complement(96..524)
                     /gene="rplM"
                     /locus_tag="PLQ54_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOS94108.1"
                     /translation="MRTYSVKPGDIKREWWVVSADGAVLGRLAARVAYILRGKHRPQF
                     TPHADCGDHVIITDCEKVRLTGNKMRDKLYYSHSRYPGHLKSANAAMMLSKHPEKVVT
                     RAVCGMLPHNRLGRQMARKLKVYVGPEHPHEAQKPKPLEV"
     gene            complement(558..1310)
                     /gene="truA"
                     /locus_tag="PLQ54_12395"
     CDS             complement(558..1310)
                     /gene="truA"
                     /locus_tag="PLQ54_12395"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOS94109.1"
                     /translation="MPRERHERILRLDVEYDGTDYCGFQIQPNGPTIQGALEAALLRL
                     TGVPIRVHGAGRTDAGVHALGQVVSFRTDYPMPDERFAEVLNRLLPESIAVWQSGRAD
                     EGFHARFSAIGKVYEYHLLRSRHRRPIASRFAEPVHPWPDVLAMNAAMECLLGRHDFS
                     GFTAAGGKANDRVRTIASAQWTENDEGAFLRIQADGFLYKMVRNIVGTALEIGIGRRP
                     VSWMAEVLDSGDRSKAGPTAAARGLRLVRVLY"
     gene            complement(1324..1725)
                     /gene="rplQ"
                     /locus_tag="PLQ54_12400"
     CDS             complement(1324..1725)
                     /gene="rplQ"
                     /locus_tag="PLQ54_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOS94110.1"
                     /translation="MRHRRAYRKLNMPTDQRLAMLRNLTKSLIVHGRIETTQTRAKEA
                     RRLAERLITLAKTDSVHNRREAGRVLGTERKLTPAERKAGIEKVDPIRKLFETIGPEM
                     KDKPGGYTRLTSIGYRRGDGVEMAVLELVPD"
     gene            complement(1751..>2436)
                     /gene="rpoA"
                     /locus_tag="PLQ54_12405"
     CDS             complement(1751..>2436)
                     /gene="rpoA"
                     /locus_tag="PLQ54_12405"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOS94111.1"
                     /translation="IRLPAEIEVVHPERHIATLTSPDASLYMEIEIQRGRGYVPAEQQ
                     PKRTEVGLIPVDSIFSPIRKANYFVEPTRLGHRTDFDRLVMEVWTDGGMEAAAAIGEA
                     ARVLDRYLRYFFEFADHEDEERAQEEEAERSKNKALEYRIEDLDFSVRTYNCLKKEQI
                     NTIAELVQRSEQDLMTIRNFGKKSLTEVCQKLAQLGLALRESLTAGEEDEDLSLDNED
                     FDLPLPDEA"
BASE COUNT          362 a          847 c          739 g          488 t
ORIGIN      
        1 gggggtcaac cacacccggg cggtagcatt cttgcgatga cccgtgccgt agtagatggc
       61 tccgtccgcc attgagtctg ttctcctccc gcccgctaga cctcgagggg tttgggcttc
      121 tgcgcctcgt ggggatgctc ggggcccaca tacaccttga gcttccgagc catctgccga
      181 ccaagccggt tgtgtggcaa catgccacac acggcccgcg tgacgacctt ctccggatgc
      241 ttactgagca tcatcgccgc gttagccgac ttcagatggc ccgggtaacg cgaatgcgag
      301 tagtagagct tgtcccgcat cttgttgccc gtgagacgga ccttctcaca gtcggtgatg
      361 atcacgtggt ccccgcagtc ggcatggggc gtgaactgcg gccggtgttt cccgcggagg
      421 atgtaggcca ccctcgccgc gagccgcccg agtaccgcgc catcggcgct cacgacccac
      481 cattcgcgct tgatgtctcc tggtttcacg ctgtaggttc tcatcgtgcc tatctccccg
      541 ctgacgcttc cgccgcccta gtagaggacc cgtaccaagc gaagtccccg ggcagccgcc
      601 gtcggccccg ccttcgaccg atccccgcta tccagtactt ccgccatcca gctcaccggc
      661 cgccggccga taccgatctc cagtgccgtt cccacgatgt tccttaccat cttgtagagg
      721 aagccgtctg cctggatccg cagaaacgcc ccctcgtcgt tctcggtcca ctgggccgag
      781 gcgatggtcc gcacccggtc gttcgccttg ccgcccgccg ctgtgaagcc cgagaaatcg
      841 tgtcggccca gcaggcactc catggcggcg ttcatcgcca acacatccgg ccacgggtga
      901 accggctcgg cgaaccggct ggcgatggga cgccggtgcc ggctccgcag caggtggtac
      961 tcgtacacct ttccgatggc cgagaaccga gcatggaacc cctcgtccgc ccggcccgac
     1021 tgccacaccg cgatgctctc ggggagcaac cgattgagca cctcggcgaa tcgctcgtcc
     1081 ggcatcgggt agtccgtccg aaacgagacg acctgaccca aggcgtggac tccggcatcg
     1141 gttcgccccg ccccgtgcac ccggatgggc actccggtca gccgaagcag cgccgcttcg
     1201 agcgccccct ggatcgtcgg gccattgggc tggatctgga atccgcagta atcggttccg
     1261 tcgtactcca catccagccg taggatccgc tcatggcgct cccgcggcac ggcggtcgcc
     1321 gcctcaatcc ggaacgagct cgagcactgc catctcgacg ccatcgccgc gccggtagcc
     1381 gatagacgtc aggcgcgtgt agccgccggg cttgtccttc atctccggtc cgatcgtctc
     1441 aaacagcttg cggatcgggt cgaccttctc gatccccgct ttccgctcgg ccggggtgag
     1501 cttccgctcg gtccccagta cccggcccgc ctcgcgccgg ttgtgcacgc tgtccgtctt
     1561 cgccaaggtg atcagccgct cggcgagccg tcgggcctcc ttggcccggg tctgcgtggt
     1621 ctcgatccgc ccatgcacga tcaacgactt ggtcaggttc cgaagcatgg ccaagcgttg
     1681 gtccgttggc atgttcagtt tgcggtatgc tctgcggtgt ctcatggtca gtcctcgtcc
     1741 cccggagcgg ctaggcctca tcgggaagtg gcaggtcgaa gtcctcattg tccagggaca
     1801 ggtcttcgtc ttcctcgccg gcggtcaggc tttcgcgcag cgccagtccg agttgtgcca
     1861 gcttctggca gacctcggtt agcgacttct tcccgaagtt gcgaatcgtc atcagatcct
     1921 gctcgctgcg ctgcacgagc tcagcgatgg tgttgatctg ttccttcttc aggcagttgt
     1981 aggtccggac tgagaagtcc aggtcctcaa tccggtattc cagagccttg ttcttgctgc
     2041 gttcggcttc ctcttcctgg gcccgctcct cgtcttcgtg atcggcgaac tcgaagaaat
     2101 accgcaggta ccggtcgagc acgcgggcag cctcgccaat ggcggccgcc gcctccatgc
     2161 ccccgtccgt ccagacctcc atgaccagac ggtcgaagtc ggtccggtgc cccagacggg
     2221 tcggctccac gaagtagttg gccttccgga tggggctgaa gatggagtcc acggggatca
     2281 gccccacctc ggtgcgcttg ggctgttgtt ccgcgggcac gtacccgcgg ccccgctgga
     2341 tctcaatctc catatagagc gaggcgtcgg gcgaggtgag cgtggcgatg tgccgctcgg
     2401 ggtggaccac ctcgatctcg gcggggaggc ggatgt
//
LOCUS       DAOSPL010000687         2435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43219_length_2435_cov_2.907143, whole genome shotgun sequence.
ACCESSION   DAOSPL010000687 DAOSPL010000000
VERSION     DAOSPL010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43219_length_2435_cov_2.907143"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLQ54_12410"
     CDS             complement(<1..392)
                     /locus_tag="PLQ54_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004052702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOS94112.1"
                     /translation="MRVHALHDLSAVVTGLLLALTLPAGLPVWQAVLGSVFAIAIAKQ
                     LYGGLGYNPFNPALVGRAFLLVSFTATMTAWTPSTWLAGSADNAFAGAKLEKAKVYGD
                     YRKLLDSEKRVDAVVMAPGQRWHVPMSKA"
     gene            381..1256
                     /locus_tag="PLQ54_12415"
     CDS             381..1256
                     /locus_tag="PLQ54_12415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS94113.1"
                     /translation="MDPQRCGKGYALALGFARVLEDPEVSVVIVVDADCRLTEGTIDH
                     LADAALRLGRPIQADNVVECRADDPLARLSVFAFRLRNRLRPRGLARLGGPCHLAGTG
                     MAFLRRHLRDAPHMCGHLAEDLALGVELALSGHVPAFCEDAGVRSDLSPTRDGREMQR
                     RRWERGHLITLGTTVPRMILGAVRRRSPQLGLGAMDLGIPPLSAHVALLLTGFGASAV
                     AMALGMSAVPAALWAGELTMVGVSVGVGYLAVGRDVLHPKDLPAISLYLATRLFSHVR
                     WTVDRKSSVWLRAKR"
     gene            1311..>2435
                     /locus_tag="PLQ54_12420"
     CDS             1311..>2435
                     /locus_tag="PLQ54_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94114.1"
                     /translation="MQRGRAPVWFGLVNPSQSVQPTAYAALLLFAPMAAILFSLLRPH
                     QAAAIAMFGAMLFLPERVGFDLPGLPPIDKRSLPPLVVLIALLLTAPRRLAPAQLLRR
                     SDWFMVVLVLGAFATAYGNSDPVPCGPRRIRGIGNWDALSEASGVVLGIVAPYLVGRV
                     QIRSSRELRDFATILVGASLVYLPLIYFELRMSPQLHNMVYGFTQHSFLQTIRFGGYR
                     PMVFMSHGLTLAMFSFAGFVVAWALTRRGTRVFGTLSVPIALVLSVTHVLLKSTGAIL
                     YALVALPLLFFVRRRTGLIVATLLCAVVLTYPSTRAAGTFPTTWLVQQAQTLSYDRAG
                     SLDFRFQNEDLLLEKARQRFWLGWSGWGRSRVCNADGSDGA"
BASE COUNT          333 a          822 c          818 g          462 t
ORIGIN      
        1 gccttgctca tcggcacgtg ccagcgctgg cccggggcca tgaccacggc gtccacgcgc
       61 ttctccgaat cgaggagctt gcggtagtcc ccgtacacct tggccttctc cagcttcgcg
      121 cccgcgaagg cgttgtcggc ggagcccgcg agccaggtgc tcggggtcca ggccgtcatg
      181 gtggcggtga aggagacgag caggaaggca cggcccacga gggcggggtt gaaggggttg
      241 tagcccaagc cgccgtagag ctgcttggcg atggcgatcg cgaagacgga gccgaggacg
      301 gcctgccaaa ccggcagtcc ggcaggcagc gtgagcgcga gcagaagccc cgtgacgacg
      361 gcgctgaggt cgtggagcgc atggacccgc aacgctgtgg caagggctac gcactggctc
      421 ttggcttcgc ccgcgtgctg gaggatcccg aggtttcggt cgtgatcgtc gtggacgccg
      481 actgccgatt gacggagggc accatcgatc atctggctga cgccgccctg cgattggggc
      541 gcccaatcca ggccgacaac gtggtggagt gcagggccga tgatccgctc gcgcggctct
      601 cggtctttgc gtttcggctg cgtaaccggc ttcgccctcg tggcctcgcg cggctgggtg
      661 gcccctgtca cctggccggg acgggaatgg cgttcctacg ccgacacctg cgcgacgctc
      721 cgcacatgtg cggacacctc gccgaggatc tggcgctggg cgtcgaactg gccctctccg
      781 gccatgtgcc ggccttctgt gaggacgccg gcgtacggag cgacctctcc ccgactcgcg
      841 acggaaggga gatgcagcgg cgacgatggg agcgcggcca cctcatcacg ctgggcacca
      901 ccgtcccacg catgatcctc ggggcggtcc gtcggagaag cccgcagctt gggctcgggg
      961 cgatggacct tggtattcca cccctctcgg ctcatgtcgc gctcctcttg accgggttcg
     1021 gtgcgagcgc cgtcgcgatg gcccttggca tgtcggctgt gcccgcggcg ctgtgggccg
     1081 gagagctcac aatggtggga gtcagcgttg gggttggcta tctcgcggtc ggacgtgacg
     1141 tgctgcaccc gaaggatttg ccggccatct ccctctacct ggcgacccgg ctcttctcgc
     1201 acgtgcggtg gacggtcgat aggaaatcgt cggtgtggct tcgagccaag cggtgagggt
     1261 gcggatcgtc gccgtcaggc caagcgttcg gttgtgactc gagacacggg ttgcagcgtg
     1321 gaagggcccc ggtatggttc gggctggtca acccttcaca gtccgtgcag cccaccgcct
     1381 atgccgcatt gctgctgttc gccccgatgg cggcgatcct cttcagcctg cttcggcccc
     1441 accaggcggc tgcgatcgcg atgttcggcg cgatgctgtt cctcccggaa cgggtcggct
     1501 tcgacctgcc cggtctgcct ccgatcgaca agcggtcact cccaccgctc gtggtgctga
     1561 tcgccctcct cctcacggct ccacgccgcc ttgcgccggc gcaactcctt cggcgtagcg
     1621 actggttcat ggtcgttctg gtcctcgggg cgtttgcaac cgcctatggc aactcggacc
     1681 cggtgccgtg cgggccgcgc aggattcggg gcatcggcaa ctgggacgca ctgtccgagg
     1741 cctctggcgt ggtgctcgga atcgttgctc cttacctcgt cgggcgcgtc cagatccgaa
     1801 gctcgcgcga actccgagac ttcgccacaa tcctggtcgg cgcgagcctc gtgtacctcc
     1861 cgctgatcta cttcgagctc cgcatgagcc cgcagctaca caacatggtc tacgggttca
     1921 cgcaacacag cttcttgcag accatccgct tcgggggcta tcggccgatg gtcttcatgt
     1981 cccacgggct caccttggcg atgttctcgt tcgccggctt cgtcgtggcc tgggccttga
     2041 cgcgccgagg tactcgcgtg ttcgggactc tctcggtccc gatcgcgctc gtcttgtcgg
     2101 tcacgcacgt tctgctcaag agcacgggcg cgatcctgta cgccctcgtc gcgctcccgc
     2161 tcctcttctt cgtcaggagg cgcaccggac tcatcgtcgc gacgctgctc tgcgccgtcg
     2221 tgctcacata cccctcgacg cgcgccgcgg gcacgttccc caccacctgg ttggtccagc
     2281 aggcgcaaac gctgagctac gaccgcgccg gatcgctgga ctttcgcttc cagaacgaag
     2341 atctcctgct cgagaaggcc cggcagcgct tctggctcgg gtggagcggc tgggggcgat
     2401 ctcgggtctg taacgccgat ggcagtgacg gggcc
//
LOCUS       DAOSPL010000688         2434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43232_length_2434_cov_3.710383, whole genome shotgun sequence.
ACCESSION   DAOSPL010000688 DAOSPL010000000
VERSION     DAOSPL010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43232_length_2434_cov_3.710383"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PLQ54_12425"
     CDS             complement(<1..417)
                     /locus_tag="PLQ54_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOS94115.1"
                     /translation="MELFHRTAHELAELLRRKELSAVELCDAVLGRIEAVEPRVRAYV
                     TVMADVAREQAKAVDARRAAGEDLPPLAGIPLAVKDNMCTVDAPTTCSSKVLEHFVAP
                     YDATVVARARQAGCVFTGKTNLDEFAMGSSTENSAFF"
     gene            complement(440..736)
                     /gene="gatC"
                     /locus_tag="PLQ54_12430"
     CDS             complement(440..736)
                     /gene="gatC"
                     /locus_tag="PLQ54_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOS94116.1"
                     /translation="MVRLSLDEVRHVARLGRLAMTDQQCEAMRDQLDSILAHFEALQE
                     LDTDDVEPTSHSLDMANVYRDDEPAPSFDRDRILANAPKRDDECFRVPLIMEED"
     gene            complement(871..2184)
                     /locus_tag="PLQ54_12435"
     CDS             complement(871..2184)
                     /locus_tag="PLQ54_12435"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOS94117.1"
                     /translation="MHFHPSAAEYILQEIQQLHEEGFDVRQLEPWADAARERPETLNH
                     QSYRELLALAQGLPRQAEFAYIEPSCLAGIGRERPEGPRVLPLAFSDVVLADRIRGAW
                     LGRTAGCMLGKPVEGWSRGDIRRLLEHAGAWPLDGYFPPVESLPEGMTFVSPSDPCLR
                     GNITHGVRDDDMDYPLIGLACLEEAGGNLTSETVANTWLHRLPAGCVYTAERAAYRNL
                     VNGISPPASGVFENPYREWIGAQIRADIWGWVSPGRPEQAARLAFRDACVSHVKNGIY
                     GEMLFAAVLAAALAGDDVEEALWAGLSEIPRVSRLTEAITDVIEWSKTDDDWPATMDR
                     VFSKYGRYHPVHTINNAAVVVCGLMHSRGDFGRAICIAVMGGIDTDCNGATAGSVMGA
                     LLGARALPAHWVDPLNDRLESIVVGMTSNRISDLAQRTLTLAKKL"
     gene            complement(2233..>2434)
                     /locus_tag="PLQ54_12440"
     CDS             complement(2233..>2434)
                     /locus_tag="PLQ54_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94118.1"
                     /translation="ARLLIGEIADGRWRTLAERTVRRRHNGFEIEFAGHEALSLAILA
                     APGREDEAIKELESDVALDWAD"
BASE COUNT          408 a          792 c          853 g          381 t
ORIGIN      
        1 gaagaaggcc gagttctcgg tggacgagcc catggcaaac tcgtccagat tcgtcttccc
       61 cgtgaagaca cagccggcct ggcgcgctcg ggcgacgacg gtggcatcgt agggcgccac
      121 gaagtgctcg agcaccttcg agctgcacgt cgtcggcgcg tcgacggtgc acatgttgtc
      181 cttgacggcc aggggtatgc ccgccagggg aggcagatcc tcgccggccg cacggcgcgc
      241 atcgacggcc ttggcctgct cgcgcgcgac atcggccatg acggtcacat aggcccgcac
      301 gcgaggctcc acagcctcga tgcgcccgag aacggcatca cacagctcga cggcggagag
      361 ctccttgcgg cggaggagtt cagccagttc gtgggcggtg cggtggaaca gctccatcgg
      421 tctgactcct aacggaggcc tagtcctcct ccatgatgag cggcacccgg aagcactcgt
      481 cgtcgcgttt gggcgcattg gccaggatcc ggtcgcggtc gaaggacggg gccggttcgt
      541 catcgcggta gacgttggcc atgtcgagcg agtgggaggt gggctccacg tcgtcggtgt
      601 cgagttcctg caacgcctcg aagtgggcca ggatgctgtc gagctggtcg cgcatggcct
      661 cacactgctg atcggtcatg gcgagccggc cgaggcgggc gacgtgccgg acttcgtcga
      721 gcgagagacg cacaatgacc acccaactcc cgggcgcggg gtatgcggaa gcacgcgctg
      781 gccatcgggg cccagcgaac aaggcccccc gcacccgcgc cacgtggtga gccggggggc
      841 actagacggt gcctgccaag cagagcgcca ttatagcttc ttcgccagcg tgagggtgcg
      901 ttgggccaga tccgagatcc gattgctggt catgccgacg acaatgctct cgaggcggtc
      961 gttgagcggg tcaacccaat gggctggcag ggctcgggca cccagaagag cccccatgac
     1021 tgatccggcc gtggcgccgt tgcagtcggt gtcgataccg cccatgacgg cgatgcagat
     1081 ggcgcggccg aagtcgccgc ggctgtgcat gagcccgcac accacaaccg cggcgttgtt
     1141 gatggtgtgc accgggtgat agcgcccgta cttcgagaac acgcggtcca tggtggcggg
     1201 ccagtcatca tccgtcttcg accactcgat gacatcggtg atggcctcgg tcagccgcga
     1261 gaccctgggg atctccgaca gcccagccca gagggcctcc tcgacgtcat cgccggccag
     1321 tgcggcggcg aggacggccg cgaagagcat ctcgccgtag atgccgtttt tgacgtggga
     1381 gacgcacgca tcccggaagg ccagccgagc ggcctgctcg ggcctgccgg gcgaaaccca
     1441 tccccagatg tcggcccgga tctgggcccc aatccactcg cgataggggt tctcgaatac
     1501 gcccgaggcc gggggcgaga ttccattgac caggttccgg taggccgctc gctcggcggt
     1561 gtagacgcag ccggccggga ggcggtggag ccaggtgttc gccaccgtct cgctggtcag
     1621 gttcccaccg gcctcctcaa gacacgccag cccgatcaga gggtagtcca tgtcatcgtc
     1681 gcggaccccg tgagtgatgt tgccgcggag gcacgggtcg ctgggcgaga cgaaggtcat
     1741 accctcgggc agcgactcca ctggcgggaa gtagccatcc agcggccacg caccggcgtg
     1801 ctcgagcagc ctccgaatat cgccacgcga ccagccctcg accggcttgc caagcatgca
     1861 gccggctgtg cggccgagcc acgcgccccg gatgcggtcg gcgaggacta cgtcggagaa
     1921 cgctagcggg agaacccggg gtccctcggg acgctcgcgg ccaatgcccg cgagacacga
     1981 cggctcgatg taggcgaact cggcctggcg gggaagccct tgggccaggg cgagcagctc
     2041 gcggtaggac tggtgattga gggtctcggg cctctcgcga gccgcgtccg cccatggttc
     2101 gagctggcgg acgtcgaagc cctcctcgtg gagttgttgg atctcctgaa ggatgtactc
     2161 cgccgcggac ggatggaagt gcatgatgga ccccccagat cggaagaagt ggaaccgggc
     2221 gacgacaggc ggtcagtccg cccagtcgag tgcgacgtcg ctctcgagct ccttgatagc
     2281 ctcgtcctcg cggccggggg cggccaggat ggccaggcta agcgcctcat ggccggcgaa
     2341 ctcgatctcg aatccattgt gtcgccggcg gaccgtccgt tcggcaagcg ttcgccaccg
     2401 gccgtccgca atctcgccga tgagcaggcg ggct
//
LOCUS       DAOSPL010000689         2432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43282_length_2432_cov_3.291544, whole genome shotgun sequence.
ACCESSION   DAOSPL010000689 DAOSPL010000000
VERSION     DAOSPL010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43282_length_2432_cov_3.291544"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLQ54_12445"
     CDS             complement(<1..259)
                     /locus_tag="PLQ54_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94119.1"
                     /translation="MTLRRVLLAGSAMAVLCSSVYGAELTLESREAPSPQATGQKALR
                     LIEVFEQLAVGPPQHMAIADLLRPIPGLREAAEAAEAEALKT"
     gene            complement(256..1320)
                     /locus_tag="PLQ54_12450"
     CDS             complement(256..1320)
                     /locus_tag="PLQ54_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94120.1"
                     /translation="MQRFTSVIAPVLLLLLAVPLWAQDVPAEPPPGQEPPPTEQPAPE
                     KPPGEQPAAPADRAEDAAIVEQARSAQRQIRMIEIIQALQLNRGQVEALADLMAQIQA
                     DKAEIAKLEGAVRERDLRRIENVVTDWTELKQIAPQDFANAQQVGAKQRADIARLRAH
                     VAALVGQAIEQVLDAEQRSGIETPQERLTKLRRTERILQNRMVDVEGLMKSLEKAVQL
                     DPEQFNQQSEALADRLAADVAGPDSPYLATVKQRMMRVLQDLKRIPQPEWQANRENIQ
                     SMICNQLGIEPPEEKGEPGRVKPDVVPQESFFVFMTDPEALPLLTKILERGQEVPPEG
                     EGEGEGEGGLGLAWVGGAAA"
     gene            complement(1358..2296)
                     /gene="serS"
                     /locus_tag="PLQ54_12455"
                     /pseudo
     CDS             complement(1358..2296)
                     /gene="serS"
                     /locus_tag="PLQ54_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003352267.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
     gene            2378..>2432
                     /locus_tag="PLQ54_12460"
     CDS             2378..>2432
                     /locus_tag="PLQ54_12460"
                     /inference="COORDINATES: protein motif:HMM:NF021230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94121.1"
                     /translation="MSLGSEYIFRGISQTAGK"
BASE COUNT          367 a          821 c          827 g          417 t
ORIGIN      
        1 ccgtcttgag ggcctccgct tcagccgcct cggccgcttc ccgaagaccg gggatcgggc
       61 gcaagaggtc ggcgatggcc atgtgctgcg ggggaccgac cgcgagctgc tcgaagacct
      121 cgatgagccg aagggctttc tgtcccgtgg cttggggtga cggggcctcg cgtgactcca
      181 gggtgagctc ggcaccgtag acggatgagc agagcacggc catggccgac cccgcgagga
      241 gaacgcgtcg gagtgtcatg ccgcagcacc tcccacccag gcgaggccca gaccgccctc
      301 gccctcgccc tcgccttcac cctccggagg tacctcctgg ccgcgctcga ggatcttcgt
      361 caacagcggc agggcctccg ggtcggtcat gaagacgaag aaggactcct ggggcaccac
      421 gtctggcttc acgcggccgg gctccccctt ctcctcgggc ggttcgatcc ccagctggtt
      481 gcagatcatg ctctggatgt tctcgcgatt ggcttgccat tcgggctgtg ggatgcgctt
      541 gaggtcctgg agcacccgca tcatccgctg cttcacggtg gccagatagg gtgagtccgg
      601 accggcgacg tcggcggcca gtcgatcggc caatgcctcg ctctgctggt tgaactgctc
      661 ggggtccagc tggacggctt tctcgaggga cttcatgaga ccctcgacgt cgaccatgcg
      721 gttctggagg atgcgctccg tgcggcggag cttggtcagg cgctcttggg gtgtttcgat
      781 tccggagcgc tgctccgcgt cgaggacttg ctcgatagcc tggcccacga gcgcggccac
      841 atgggcccgg agccgggcga tatcggcccg ctgtttggcg ccaacctgct gagcgttggc
      901 gaaatcctgc ggcgcgatct gcttgagctc ggtccagtcg gtgaccacgt tctcgatccg
      961 gcgtagatcg cgctctcgga cggccccctc gagcttggcg atctcggcct tgtcggcctg
     1021 gatctgggcc atgagatcgg cgagggcctc gacctggccg cggttgagct gtagtgcctg
     1081 gatgatctcg atcatccgga tctgacgctg ggcggagcgc gcctgctcca cgatcgccgc
     1141 gtcctccgcc cgatcggcag gggcggcggg ctgctctccc ggcggcttct ccggggccgg
     1201 ttgctccgtg ggcggtggct cctggccggg cgggggttcg gccggcacat cctgggccca
     1261 caggggcacc gcgaggagca gcaacagcac tggcgcgatg actgaggtga accgctgcac
     1321 tctggctccc tccctacgtc tcgcccgtcc aagacctcta cgcgggcgtg atcctgtcca
     1381 ggccacccat gaacggccgg agcgcctcag gaacgacgac cgacccgtct gcttgctggt
     1441 agttctcgag aatggcagca aaggttcggc cgacggcgag ccccgatccg ttgagcaagt
     1501 gcacaaactc gggcctgccg cccggctcgc gccggaagcg cacttgcccc cggcgagcct
     1561 ggtagtcgcc gcagtcggaa cacgatgaga tctcgacata gcggcccatg ccgggcatcc
     1621 agacctcggg gtcgtaggtc cggacggcgt tggggccgat gtcggcggtg cacagctcca
     1681 tgacgcgata ggggagcccg aggccttgga ggacatcctc ggcagccgcg agcaggcgct
     1741 gcagctcggc cggggagtcc tcgggcttgc agtagacggc catctcgacc ttgtcaaact
     1801 ggtgccggcg gatcagcccc ttggtgtcgc ggcccgcggc gccagcctcg gcccggaaac
     1861 agggggtgta ccccgtgtag cggatgggga gctgaccgcc atcgagaatc tcgttggcat
     1921 ggtagccaca gaggggcacc tcggccgtag gaataaggaa tagatcacta ttagccactc
     1981 ggaacatgtc ctcttccatg gccgggacct gtccggtgca ctgcatggcc tgccgcgtcg
     2041 caaggaaggg gggggagatc tcggtgtacc cgtggtgggc cacgtgcaga tggatcatgt
     2101 agctaatcag cgcgcgctcc atggctgcac cggctccccg aaacagcgcg aagcgagcgc
     2161 cggccagctt ggaggcgacg tcgaagtcga ggacgcccag ttcggggccc agatcccagt
     2221 gcgccttggg ctcaaagtca aacctcggga tctcccccca ccgccggacc tccacgttgt
     2281 ccgcctcgct ctcgcctctg ttgcgctcgc gagctccgcc gccttcgccc aggcccccgc
     2341 cgcgccggcg acgcccgacc acaccgtcac cgccaacgtg tcgctgggca gcgaatacat
     2401 cttccgcggg atctcgcaga ccgcgggcaa ga
//
LOCUS       DAOSPL010000690         2432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43289_length_2432_cov_2.468658, whole genome shotgun sequence.
ACCESSION   DAOSPL010000690 DAOSPL010000000
VERSION     DAOSPL010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43289_length_2432_cov_2.468658"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..>2432)
                     /locus_tag="PLQ54_12465"
     CDS             complement(157..>2432)
                     /locus_tag="PLQ54_12465"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOS94122.1"
                     /translation="KGSISVSSTPSGYAIDLDGHERGQTPASLGNIPAGYHRVALRVE
                     GRLLKPQWVVVKPGEQVVVAIDAAGAPPPPPPKVAEGRSGLVCTVSDLDDCEYFIDNE
                     YKQRKSEVGPMVVRDVRPGDHTVRIRFEIDGKRVQVKRKVTLEPDRVETIDIRALEEL
                     STLDIATDLDINHVYVGEDRWTLVHIAQGKGSLDPVGSGEQPVEVEYQNAVVRVPVAI
                     EAHKPAAIELLRKNLFGDITVTVDLDEARALLDGAALEVGAGRTSQTVVVAPGEHELE
                     YRLGEDVIDRKTVTVAAGETATVSFGWKQYFGELSITAKAPDATLTVDGRPYEFSAAE
                     PLVLRLKPGTYDVALRGAKIDDKQQVTVEAGREAKVAFKTTSLRLVVGLAAKTVRVDG
                     KSYEPVNGVVEINGIEPGTHKVEIKDLRFLGDIDVPDGVDVVRDFTQGGGATTVPTAK
                     VEFASVPDKLELSIAPAAKPNDLKPMGTTPCAVELPVGSYRVVAKNAGFKAAETIEVK
                     SAAPMSVELVAAPMGMIFIPGGRFTMGHDAGPADQRPGHSVRLNGYFIDEYPVINEEY
                     AVFAAETGRPAPAGDPQAPVTNVTWDDAKAYAEWCGKRLPTEAEWEHAARGLDNAFYP
                     WGPDFDPSRCNCLPPGTAADTRKKRDREAEGQPVAAGNVEPIGKREGNVSTFGVCDMA
                     GNVWEWVQDWYGPYQRDRDNPLGPPQGKERVIRGGAYDSTPEECMSIYRGHVVPGTRA
                     PNIGFRCALTARADEGQ"
BASE COUNT          408 a          872 c          694 g          458 t
ORIGIN      
        1 gagagccacc agcagcgcac acgtcgccct agtcggtctc agtgccattc agctacctcc
       61 cacttcccac catggagaga gcaatccggt agacgggaaa ccccgtccga tggcctcaaa
      121 ccgcgcgcac accgacccgc gtccctgtcc tatgacctac tgaccctcgt cggcacgtgc
      181 cgtcagtgcg cagcggaatc cgatgttcgg tgccctcgtc cctgggacca catgcccgcg
      241 gtagatcgac atgcactcct caggcgtgct gtcgtaggca ccgccacgta tcaccctctc
      301 cttcccctga gggggaccca gagggttgtc ccgatccctc tgatatgggc cgtaccagtc
      361 ctgcacccac tcccagacgt ttcctgccat atcgcaaacg ccgaatgtac tgacattgcc
      421 ctcgcgcttc ccgatgggct ccacgttgcc cgcggccacc ggctggccct cagcctcccg
      481 gtcgcgcttc ttccgggtgt cggcagcggt gccgggggga aggcagttgc agcgtgacgg
      541 gtcgaagtct ggaccccacg ggtagaaggc gttgtccagc ccccgcgcag cgtgctccca
      601 ctccgcctca gtgggaagtc gcttgccgca ccattcggca taggccttcg catcatccca
      661 cgtgacgttg gtgaccggag cttgcggatc acccgcaggc gctggccggc cggtctcggc
      721 agcgaagacc gcatactcct cattgattac gggatattca tcaataaagt aaccgttgag
      781 ccgcaccgaa tgtcccggcc gctggtcggc cggccctgcg tcatggccca tggtgaaacg
      841 gcccccgggg atgaatatca tccccatcgg cgctgcgacc agctccacgg acatgggagc
      901 cgcactcttc acctcgatgg tctccgccgc tttgaagccg gcgttcttgg ccaccacacg
      961 gtaggatccg accggaagct ccaccgcgca cggcgttgtg cccatcggct tgaggtcgtt
     1021 cggcttcgcg gccggagcga tgctcagctc cagcttgtcg ggtaccgagg cgaactccac
     1081 cttggccgtg ggtaccgtcg tcgccccgcc accctgtgtg aagtcgcgga ccacatccac
     1141 gccgtcaggc acatcgatat cacccaggaa ccgcagatcc ttgatctcaa ccttatgcgt
     1201 gccgggctcg ataccgttga tctcaacgac gccgttcacc ggctcgtagc tcttgccgtc
     1261 cacccggacc gtcttggccg cgaggcccac caccaggcga agtgacgtcg tcttgaacgc
     1321 caccttggcc tccctgccgg cctctaccgt aacctgttgc ttgtcgtcga tcttcgctcc
     1381 tcgcagcgcc acatcgtacg tgcccggctt cagacggagc acgagaggct ccgcagccga
     1441 gaactcgtac ggtctgccgt ccaccgtgag agtcgcgtcc ggcgccttgg ccgtgatcga
     1501 cagctcgcca aagtactgct tccagccaaa gctcaccgta gccgtctcgc cggcggccac
     1561 ggtgacagtc ttgcggtcga tgacgtcctc gcccagtcta tactcgagct catgctcgcc
     1621 gggggccacc accaccgtct gactcgtacg ccccgcaccg acctccaacg cggcaccgtc
     1681 caacagcgct cgcgcctcat ccaggtcgac cgttacggtg atgtctccga agaggttctt
     1741 gcggaggagc tcgatggccg ctggcttgtg cgcttcgatg gccaccggga cgcgcaccac
     1801 ggcattctga tactcgactt ccacgggctg ttcgccacta ccaacggggt caagggatcc
     1861 cttcccctgc gcgatgtgga caagagtcca ccggtcctca ccaacgtaga cgtggttgat
     1921 atccaggtcg gtcgcgatat cgagtgtcga cagctcctcg agagcccgga tgtcgatggt
     1981 ctcaacgcgg tcgggctcga gagtgacctt gcgcttcacc tgaacgcgct tgccatcgat
     2041 ctcgaaccgg atccggaccg tatggtcgcc gggccgcaca tcgcgtacga ccataggccc
     2101 gacctcgctc ttgcgctgct tgtactcgtt gtcgatgaag tactcgcagt catccaggtc
     2161 gctgaccgtg cacaccaacc ccgatcgtcc ctccgccacc ttcggaggcg gcggaggggg
     2221 ggctccagcc gcatcgatcg ccacgacaac ctgctcgccg ggcttgacga ccacccactg
     2281 gggcttcagc agtctgccct cgaccctcag tgcaaccctg tggtaaccgg ccggtatgtt
     2341 gcccagagac gctggggtct ggccccgctc gtggccatcg aggtcgatgg catagccgga
     2401 aggcgtgctc gacacgctga tcgaaccttt ga
//
LOCUS       DAOSPL010000691         2431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43314_length_2431_cov_2.515572, whole genome shotgun sequence.
ACCESSION   DAOSPL010000691 DAOSPL010000000
VERSION     DAOSPL010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43314_length_2431_cov_2.515572"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1364)
                     /gene="ahcY"
                     /locus_tag="PLQ54_12470"
     CDS             complement(105..1364)
                     /gene="ahcY"
                     /locus_tag="PLQ54_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOS94123.1"
                     /translation="MVDHHVADLALAGKGKLRIEWAEMRMPVLRLIRARFEREKPLAG
                     AKLAACLHVTTETANLMRTLVAGGAEVRLCASNPLSTQDDVAASLVADFGIPTFAVCG
                     EDRDTYYSHIQSALAFGPTLTMDDGADVVSTLHKEGGARLEGIVAGTEETTTGVIRLR
                     AMERDGALRYPIIAVNDADTKHLFDNRYGTGQSSIDGILRATNILLAGQTVVICGYGM
                     CGRGCARRAQGMGAHVVVCEVDPLRGLEAVMDGYRVMPIAEAAKIGDVFITVTGCCSV
                     VRREHFSTMKDGAIVCNSGHFDVELEVAALREMASSSREIRQFVTEYTLDNGRRVNVI
                     GEGRLVNLAAAEGHPASVMDMSFANQALCAEYALKNSTSLQNRVYSVPREIDEDIARL
                     KLEAMGVEIDRLTPEQQRYLSSWEEGT"
     gene            1590..>2431
                     /locus_tag="PLQ54_12475"
     CDS             1590..>2431
                     /locus_tag="PLQ54_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007128547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOS94124.1"
                     /translation="MSVGGALWSAAEAGHTMFVPDLDVPRTRFSRRDLLRGLAGLSLA
                     VEAAGAANAQEETMPERPSFVLFYTDDQGYGDLGCMGAVDLSTPHLDSLAASGALLRQ
                     WYSNSPVCSPSRASVLTGRYPFHAGVRSILGGHRQATGLPPTVPTIAEALKPLGYHTA
                     MVGKWHLGVAEEFRPHHHGFDEWYGFLAGCIDYYSHIFYWGGQPVHDLWDNNREVWDN
                     GRYFTDVVGERAVGYIDRVAPTGDPFFLYVPFSAPHYPMHAPARYLDRFPGLPWERRI
                     MAAML"
BASE COUNT          385 a          805 c          824 g          417 t
ORIGIN      
        1 accgccagcg gcacgccaac ggccagtccc gggctgctca tgccaccact cactcatctt
       61 gccagtatgg atagccggga acaggtcccc ctcaggggcg cacatcaggt gccctcttcc
      121 catgaggaca ggtatctctg ctgttccggg gtgagtcgat cgatctccac acccatggcc
      181 tccagcttga gccgggcaat gtcctcgtcg atctcgcggg gcacggagta gacgcggttc
      241 tggagggagg tgctgttctt gagcgcgtac tcagcacaga gcgcctggtt ggcgaagctc
      301 atgtccatca cgctggcggg gtggccctcg gctgcggcca ggttcacgag ccggccctcg
      361 ccgatgacat tgacgcgccg gccgttgtcg agcgtgtact ccgtgacgaa ctggcggatc
      421 tcgcgggaac tgctggccat ctcgcggagc gcggccacct cgagctcgac atcgaaatgc
      481 cccgagttgc agacgatcgc gccgtctttc atggtggaga agtgttcccg ccggaccacg
      541 gagcagcatc ccgtcacggt gatgaagaca tcgccaatct tcgcggcctc agcgatgggc
      601 atcacgcggt acccatccat cacggcctcc agcccgcgca gcgggtcgac ctcgcacacg
      661 acgacgtggg cccccatgcc ttgggcgcgc cgggcgcagc ctcgcccgca catgccatag
      721 ccacagatga cgacggtctg accagcgagg aggatgttcg tggccctgag gatgccatcg
      781 atcgagctct gaccggtgcc gtaccggttg tcgaacaggt gcttggtgtc ggcatcgttg
      841 acggcgatga tcgggtacct gagtgcaccg tctcgctcca tggcccgcag gcggatgacg
      901 ccggtggtgg tttcctcggt gccggccaca atgccctcga gccgcgcgcc gccttccttg
      961 tgcagggtcg ataccacatc ggcgccatcg tccatggtga gcgttggccc gaaggcgagt
     1021 gccgactgga tgtgcgagta gtatgtatcg cggtcctcac cacagaccgc gaaggtcggg
     1081 atgccaaagt cggccaccaa tgatgcggcg acgtcatcct gcgtcgaaag cgggttggag
     1141 gcgcacagcc gcacctcggc tcctccggcc accagggtcc gcatcaggtt ggcggtctcg
     1201 gtggtgacat gaagacaggc ggcgagcttg gcaccggcga gaggcttctc gcgctcgaaa
     1261 cgcgcccgga tgaggcgcag cacgggcatg cgcatctcgg cccactcgat gcggagcttg
     1321 cccttgccgg cgagcgcgag atcggcgacg tggtggtcga ccatacgagt tcccatcccc
     1381 tatccggtgg cggtgaacgc ccacccagcg taagaggcca gtataggcag ggcccaaggg
     1441 gtggtcaaac ggcgaggcga ggccgcgcag ggccatgatg ctctcggggg gggctccgcg
     1501 tacgcggaaa cggccgcagg ctggccggac gccagttggc gggggcccac cagtgcctga
     1561 ggaggctcaa agcctggcgg aagaacccag tgagcgtggg aggcgccctg tggtcggctg
     1621 cggaagccgg tcacaccatg tttgtgccgg atctcgatgt gccgcggacc cgcttctcgc
     1681 gccgcgacct gctgcgcggc ctggcgggcc tgtcgctggc agtcgaagcc gccggggcgg
     1741 cgaacgccca ggaggagacc atgccggaac gccccagttt cgtgctcttt tacaccgacg
     1801 accaggggta cggggatctc gggtgcatgg gtgcggtcga cctgtccacg ccccacctcg
     1861 actcgctcgc tgcatcgggc gcgctgctga ggcagtggta ctcgaactcg cccgtgtgct
     1921 cgccctcgcg agcgtcggtg ctgacggggc gctatccgtt ccacgccggc gtgcggtcga
     1981 tcctcggcgg ccaccggcag gccacggggc tgccgccgac ggtgcccacg atcgcggagg
     2041 cgctgaagcc cctgggctac cacacggcga tggtcggcaa gtggcacctt ggtgtggccg
     2101 aagagttccg cccgcaccac cacgggttcg atgagtggta cggcttcctc gccgggtgca
     2161 tcgactacta ctcccacatc ttctactggg gcggccagcc ggttcatgac ctgtgggaca
     2221 acaatcgcga ggtctgggac aatggtcgct acttcaccga cgtggtcggc gagcgggcgg
     2281 tgggttacat cgatcgggta gccccgacgg gtgacccgtt ctttctctat gtccccttta
     2341 gcgcgccgca ctaccccatg cacgcgcccg cccggtacct cgaccgtttt cccggcttgc
     2401 cgtgggagcg gcgcatcatg gcggccatgc t
//
LOCUS       DAOSPL010000692         2430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43328_length_2430_cov_3.375579, whole genome shotgun sequence.
ACCESSION   DAOSPL010000692 DAOSPL010000000
VERSION     DAOSPL010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43328_length_2430_cov_3.375579"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2430
                     /gene="fliD"
                     /locus_tag="PLQ54_12480"
     CDS             <1..>2430
                     /gene="fliD"
                     /locus_tag="PLQ54_12480"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOS94125.1"
                     /translation="ELLDNLEAVDGMKAALVGGRLQVYAEDPRKDFGLGEAGGLTTGT
                     ITKVLFGLNAAQFTQSQPSGTAVVTAASTDEYTVADTVIPLHGGSPSFHTATGAEGTG
                     LDYLFTGGILYGTGINRSFVNGEESLYTSAEISAGVDTAARVDGGYNVGSSGTSLVTE
                     PSSTLVARSIFPDAKAGRYTIEYNYDGAPSAWHYRILNADGEVQGNGVLGSSTTPATF
                     ASYIDSDLAGSTFTHTYPGAGPPASGQTFEFVLKLDTSASLADAGFATPATTATNGVF
                     TINGVAIQISDYTTETVESILAKINGSGAGVVARYDSRDDRFIVASTTIGSSGTVLLG
                     SNTDSSNFLQIAKLTTTTGGTAVAGVDNNNVSLTTQLGTASTNLTTPVTSGTFTINGV
                     TLYVDASKDTLQDVIDRINGSGAHVKASYDSSTDRFVLQSDMDDESTTTNTTRIRVGA
                     AGDSSNILQALHIGTASQQQTTRYVGEAGTDAKFTFDGTTYTRVSNTVTNLVEGLTLT
                     LLAASDKTLTINVEVDTDRGMNALADFVAKYNEIVDLLNPAKLTDDQKKYLTPLTDED
                     RANMTYTEIETYTTLYEKYRRQELIRTDSALRRIMLALRRAVTSSVSGLPESMNGLID
                     LGIDTYLAPGKVVTDGTLVYDSTDKDEILKKLGDIGDLEEMLRTREDAILQLFASEEY
                     TGSGTSVLATYLVDRDSGLMVPADGQALSFRVGDGINQSSSITLQAGRTYTQAQILEL
                     LDRAGLNVGDGDDFGDRVRVTASFDSQGRLLLRCTGSGDSRYQVVLRDDSRGTNTLAT
                     VFGIDISSDGKG"
BASE COUNT          482 a          808 c          745 g          395 t
ORIGIN      
        1 ggagctgcta gacaacctgg aggccgtcga tggcatgaag gccgccctgg tgggcgggcg
       61 gcttcaggtc tacgccgagg accctcgcaa ggactttgga ctcggtgagg ccgggggcct
      121 cacgacgggg accattacca aggttctctt cggcctcaat gccgcccagt tcacccagtc
      181 gcaaccatcg ggcaccgccg tggtgacggc cgcctcgacc gatgagtaca ccgtggccga
      241 tacggtgata ccgttgcacg ggggctctcc ctcattccac acggccaccg gggccgaggg
      301 aacaggcctc gactatctct tcaccggagg cattctctac ggaaccggca tcaatcggtc
      361 ctttgtgaat ggcgaggaga gtctgtacac cagcgccgag atctcggccg gagtcgacac
      421 ggcggcccgc gttgacggcg gctacaacgt cggcagcagc gggacgtcgc tggtgaccga
      481 gccgtcgtcg acgcttgtcg cccgcagcat cttccccgac gccaaggcgg gccgctacac
      541 catcgagtac aactacgatg gtgccccctc ggcctggcac tatcgcattc tgaacgccga
      601 cggcgaggtc cagggaaacg gggtgctcgg gagcagcacc acccccgcca ccttcgcgtc
      661 ctacatcgac tcggatctgg cggggagcac cttcacccac acctaccccg gcgcggggcc
      721 gccggcatcg ggtcagacct tcgagttcgt gctcaaactg gacaccagcg cctctctcgc
      781 agacgcgggc ttcgccacgc cggcgaccac agcaaccaac ggggtcttca ccatcaatgg
      841 tgtggccatc cagatctcgg actacaccac cgagaccgtc gagagcatcc tggccaagat
      901 caacggctcg ggcgcgggag tcgtggcccg ctatgacagc cgggacgacc ggttcatcgt
      961 ggcctcgacc accatcgggt cctcggggac cgtgttgctc gggtcgaaca cggactcgag
     1021 caacttcctg cagatcgcga agctcaccac gacgacggga ggcacggctg ttgccggagt
     1081 cgacaataac aacgttagcc tgaccaccca gctgggaacg gcctcaacca acctgaccac
     1141 ccccgtcact tcgggcacgt tcacgatcaa cggcgtgacg ctctacgtgg acgcgtccaa
     1201 ggacaccctc caggacgtca tcgaccgcat caacgggtca ggcgctcatg tcaaagcatc
     1261 ctatgactcg tccaccgacc ggttcgtgct gcagagcgat atggatgatg agtcgaccac
     1321 gaccaacacg acccgcatcc gggtgggcgc ggcgggcgac tcgtcgaaca tcctccaagc
     1381 gcttcacatc ggcacggcat cgcagcagca gactacgcga tatgtcggcg aggctggcac
     1441 ggatgcgaag ttcacctttg atggcaccac ctacacccgt gtcagcaaca cggtcaccaa
     1501 cctggtcgag ggactgaccc tgacgctgct cgcggccagc gacaagaccc tgacgatcaa
     1561 cgtcgaagtc gacaccgatc gcggcatgaa cgcgctggcg gacttcgtcg ccaagtacaa
     1621 cgagattgtc gacctcctca acccggcgaa gttgacggac gaccagaaga agtacctgac
     1681 gccgttgact gatgaagacc gtgccaacat gacgtacacg gagattgaga cgtacacgac
     1741 tctgtacgag aagtaccgcc ggcaggagct gatccggacc gattcggcct tgcggcgcat
     1801 catgctcgct ctgcggcggg ccgtcaccag ttcggtcagc gggttgcccg agtccatgaa
     1861 tgggctcatt gacctgggca tcgacaccta cttggccccc ggcaaggtcg tcacggacgg
     1921 cacgctggtc tacgactcga ccgacaaaga cgagatcctc aagaagctcg gcgacatcgg
     1981 cgatcttgag gagatgctac ggacccggga agacgcgatc ctccagctgt tcgcatccga
     2041 ggagtacacg ggcagcggga ccagcgtgct ggccacctat ctggtggacc gcgattcggg
     2101 gctgatggtg ccggccgatg gtcaggccct ctcgttccgt gtgggagacg gcatcaacca
     2161 gtcttcgagc attaccctgc aggcgggccg cacctatacc caggcgcaga ttctggagct
     2221 gctcgaccgc gcgggtctca acgtgggcga tggcgacgac ttcggcgacc gcgtcagagt
     2281 tacggcctcc ttcgattccc agggccggct gttgctcagg tgcacgggct caggcgattc
     2341 acgctaccag gtcgtgctcc gggatgacag ccgcgggacc aacacactgg ccaccgtgtt
     2401 cgggatcgac atctcgtcgg acggcaaggg
//
LOCUS       DAOSPL010000693         2429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43348_length_2429_cov_3.272957, whole genome shotgun sequence.
ACCESSION   DAOSPL010000693 DAOSPL010000000
VERSION     DAOSPL010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43348_length_2429_cov_3.272957"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..>2429
                     /locus_tag="PLQ54_12485"
     CDS             97..>2429
                     /locus_tag="PLQ54_12485"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOS94126.1"
                     /translation="MAGALVMVAMAATYVAAPAAMRVDVDVDHPVQPGPNLIANGGFE
                     DVEGGQPVGWQWSPRNTDATFALVEPGRRGGKCVRIRNTTPLSPHVYGQLVLSEPVPL
                     APGETYVLSGYSQSIDPGPAWVGGGDGWWMRIPLAGTAGRWRRFSRTFVAREGDVRFA
                     LMINTDGPTEGFLVDDVKLELGSTPTPVVRDDADTSLTADLDFESPVDVPGDEWSFSV
                     LLSAPSAAPDCVVRCEARDEGGTLIAVGESPADLRAGVTAAHVTCPLAGMANVTGMLS
                     VSLTAGDGRSASDEAPVAVYTRAGFRERFARVAPAVEALRAAVTRARNAGRNVAYPIA
                     ALQVADRFGPVVEATADAGNVRLAQVYSDDLVRICETATRQVAGIEQGAPDPSVPDPP
                     MGRLRIRGRNLEADGVVVNLVGPLGYGELYDELDTVRNYGFNVVGDDFDAFASLSMVT
                     GPDTIDETAVPRLRANWDRLHALNLAISFNPTLHYFPEWAITRYPDITGGDPVDRVPD
                     WSGLGQDRGQRTKVYGGFFPFAIESPTVKHLVERYYAALLPGIRDHESFHLYWLMNEP
                     TYRSDDPEYRSAYHRFLREKYGEVSALNERWGTGFESFDAIPPAREPADPGRYDFMTF
                     HRAHVADWFTMLATEVRRHDPAAILSNKPMAWTIMQPDDGIDFERQAEILDIPGCDAG
                     RSPGSSEYAFAWDGATFLFDFYSSVAPDKPQADLEYHYVHEPEVAAEYVRASYWQSYL
                     HGLRVSTFWVWARGQLGSGEAGAGMTHTAWSQPNVGWG"
BASE COUNT          373 a          815 c          862 g          379 t
ORIGIN      
        1 ctcactgccc cgcctccgac gcccctgacg gcgttccggc gggctcggca tgcccggggc
       61 cacgacccca tcagtgttca tagggaggcc ggatcgatgg ccggggcgct cgtaatggtc
      121 gcgatggccg caacgtacgt ggcggcccca gcggcgatgc gcgtcgacgt ggatgtcgac
      181 cacccggttc agccggggcc gaacctgatc gcgaacggcg ggttcgagga cgtcgagggt
      241 ggccagcccg tcgggtggca gtggagcccc cgcaacacgg acgccacctt cgcgttggtg
      301 gagcccggcc ggcgcggcgg gaagtgtgtc cgcatccgca atacgacgcc cttgagccca
      361 cacgtgtacg gacagctcgt gctctccgag ccggtgcctc ttgcaccggg cgagacgtac
      421 gtgttgagtg gctactcgca gtccatcgac ccgggcccgg cgtgggtcgg aggcggcgac
      481 ggctggtgga tgcgtatccc gctggccggc acggcgggtc gatggcgacg gttcagccgg
      541 acgttcgtgg cgcgcgaagg cgacgtccgg ttcgcgctga tgatcaacac cgacgggccc
      601 accgaggggt tcctggtgga cgacgtcaag ctcgagctcg ggagcacccc tacgcccgtg
      661 gtgcgcgatg acgcggacac ctccctcacg gccgatctgg acttcgagag cccggtggat
      721 gtgccgggcg acgagtggtc gttctccgtg ctgctctcgg cgccgtcggc cgcgccggac
      781 tgcgtggtgc gctgcgaggc ccgagacgag ggcggcacgc tcatcgccgt gggcgagagc
      841 ccagccgacc tgcgcgccgg ggtcacggcg gcgcacgtga cgtgtcctct ggcggggatg
      901 gcgaatgtca cgggaatgct gtcggtctcg ctgaccgctg gcgacggccg ctcggcgagc
      961 gacgaggcac cggtggcggt ctatacgcgg gccgggttcc gcgaacgctt cgcacgagtc
     1021 gcgccggccg tcgaggctct ccgcgcggcc gtgacgcgcg cccggaatgc cgggcgaaac
     1081 gtggcgtacc ccattgcggc gctgcaagtg gcggatcggt tcggcccggt cgtcgaagcg
     1141 acggcagatg ccgggaacgt gcgtctggcc caggtctatt ccgacgacct ggtccgcatc
     1201 tgcgagacgg cgacccgcca ggttgcgggg atcgaacagg gcgcgcccga tccgtctgtc
     1261 cccgatcctc ccatggggag gctccggatc cgcggccgga acctcgaggc ggacggggtg
     1321 gtcgtgaacc tggtgggccc gctcggatac ggcgagctct atgacgagct ggacaccgtg
     1381 cgcaactacg ggttcaacgt cgtcggcgat gacttcgacg ctttcgcctc gctgtcgatg
     1441 gtgacgggac ccgacacgat cgatgagacg gccgtccccc ggcttcgtgc gaactgggac
     1501 cgcctccacg cgctcaacct ggccatcagc ttcaacccga cactgcacta cttccccgag
     1561 tgggccatca cgcgctaccc ggatatcacc ggcggggatc cggtggaccg cgtgcccgac
     1621 tggagcgggc tggggcagga ccgcgggcaa cggaccaagg tctacggcgg gttcttcccc
     1681 ttcgccatcg agtcaccgac cgtgaagcac ctggtcgagc ggtactacgc ggcactgttg
     1741 ccgggcatca gggaccacga gagcttccac ctgtactggc tgatgaacga gcccacctat
     1801 cgcagcgacg atcccgagta ccggagcgcc tatcatcgct tcctgcggga gaagtacggt
     1861 gaggtctccg ccctcaacga acgatggggc accggcttcg agtcgttcga cgccatccct
     1921 ccggcgcggg aacccgcgga cccggggcgc tatgacttca tgaccttcca ccgcgcgcat
     1981 gtggccgact ggttcacgat gctggccacc gaggtgcggc ggcacgaccc ggcggccatt
     2041 ctgtcgaaca agcccatggc ctggaccatc atgcagcccg atgacggaat cgacttcgaa
     2101 cgccaggcag agatcctcga cataccgggc tgcgacgcgg gccggtcgcc cggatccagc
     2161 gagtatgcct tcgcatggga tggcgccacg ttcctgttcg acttctacag ctcggtggcg
     2221 cccgacaagc cgcaggcgga tttggagtac cactatgtcc acgagcccga ggtcgcggcc
     2281 gagtacgtgc gggcttcgta ctggcagtcg tacctccatg ggctacgtgt cagcaccttc
     2341 tgggtatggg cgcgcggcca actgggcagc ggcgaggccg gagccggcat gacccacaca
     2401 gcgtggagcc agccgaacgt ggggtgggg
//
LOCUS       DAOSPL010000694         2429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43350_length_2429_cov_3.109099, whole genome shotgun sequence.
ACCESSION   DAOSPL010000694 DAOSPL010000000
VERSION     DAOSPL010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43350_length_2429_cov_3.109099"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="PLQ54_12490"
     CDS             complement(<1..1076)
                     /locus_tag="PLQ54_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94127.1"
                     /translation="MSVLLAVALLHVGQPNTLEFHVSPKGSDTHAGTARAPFASIERA
                     KEAVREAVARRLTTNVDVVIHEGTYELSRPLLFTPADGGTERFRVRYVAARGDRVTFS
                     GGSRISGWRRGDGGVWTAHAPWAFSQLVVNGRRAVRARTPDLGATPEYMELARADLAE
                     DRSLYTLATQPGQLADWEHPQDIEVVVLGNWEITRKRVQAIDPMTNTIVLAPPHAGGH
                     DAIRPGPGRFYFLENAREFLGEPGEWYLNEGTGEVSYLPRPGEDMATAGVIAPRLTRL
                     IEVRGTEGHPVRNLHFQGIRFAHADWVMPDFGFNGIQASFHCIPTEGARGWAWDTWEC
                     IEPAIRFEFADSCTLTDGALEFLRG"
     gene            complement(1164..2249)
                     /locus_tag="PLQ54_12495"
     CDS             complement(1164..2249)
                     /locus_tag="PLQ54_12495"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HOS94128.1"
                     /translation="MKILVIGSGGREHALAWRLAQSPRTQKVFVAPGNAGTARELGLQ
                     NVPISDVATLAAFAEREPLYGWRSDSPEAMDQALGLMASHGIDFIVFLWYERGAWRFD
                     DSHPSDLMDTGLDLYLRTPNRHAVGFCLMWTQPVPADRLDAAVAEWLGCAADPDYTRI
                     DGRPALFVYADVLDSTPGGDEALRDQITRVRAAAAKADLPGLYIVANHFSIGQADRFR
                     ALGFDAVSSYVNFGGQPGANPYSALVDSVRATWDSAPAGLPYIPNVTTGWDPRPRMGF
                     MDTYDRWFEGRTPDALREYVAAALDWVATHPDRVPSSPLVTLYAWNELDEGGWLVPTR
                     GEGPAMLEAVRDAVQACRWGTSAERRR"
     gene            complement(2300..>2429)
                     /locus_tag="PLQ54_12500"
     CDS             complement(2300..>2429)
                     /locus_tag="PLQ54_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94129.1"
                     /translation="LAKAGATAVIQPGGSMRDAEVIAAADEQDIAMVFTGFRHFRH"
BASE COUNT          396 a          815 c          850 g          368 t
ORIGIN      
        1 ccacgcagga actcgagcgc cccgtctgtc agcgtgcacg agtcggcgaa ctcgaaccgg
       61 atggccggct cgatgcactc ccacgtgtcc catgcccagc ccctggcccc ctccgtcggg
      121 atgcagtgga acgacgcctg gataccattg aagccaaagt cgggcatgac ccagtcggcg
      181 tgggcgaagc gaatgccctg aaagtgcagg ttccgcacgg gatggccctc ggtcccgcgc
      241 acttcgatca accgcgtcag ccgcggggcg atcaccccgg ccgtcgccat gtcctcgccc
      301 ggccggggca gataggagac ctctcccgta ccctcgttca ggtaccactc gcccggctcg
      361 ccgagaaact cgcgggcgtt ctcgaggaag tagaagcggc ccgggccggg ccggatggcg
      421 tcatgtccac cggcgtgcgg cggtgccagc acgatcgtgt tggtcatcgg gtcgatggcc
      481 tgaacgcgct tccgggtgat ctcccagttc ccgaggacca cgacctcaat gtcctgcgga
      541 tgctcccagt cggcgagctg gcccggctgg gtcgcgagcg tgtagaggga ccggtcctcg
      601 gccaggtcgg cgcgtgccag ctccatgtac tcgggtgtcg cgcccaagtc gggtgtgcgg
      661 gccctgacgg cgcggcggcc gttgaccacg agctggctga aggcccaggg cgcgtgcgcc
      721 gtccacacgc cgccgtcgcc gcgacgccag ccactgatgc gactcccgcc gctgaaggtg
      781 acccggtcgc cccgggccgc cacatagcgc acgcgaaagc gctcggtgcc gccgtcggcg
      841 ggagtgaaga gcaacggccg gctgagctcg taggtgccct catggatcac aacgtccacg
      901 ttcgttgtga gcctgcgggc cacggcctcg cgaaccgctt ccttggcccg ttcgatcgaa
      961 gcgaagggcg cccgggcggt gccggcgtgg gtgtcgctcc ccttgggtga cacgtggaac
     1021 tcgagtgtgt tgggttggcc cacgtgcaac agagccaccg caagcagtac ggacatggca
     1081 ggtccttccc acaaacgccg gtgctggcgc gaggagttcg cccaaggagc ggaaggcccc
     1141 tcgcgggact ggaagaccag gcgtcagcga cgacgctcgg cagacgtgcc ccatctacat
     1201 gcctgcacgg cgtcacgcac ggcctcgagc atggcgggac cctcaccgcg cgtgggaacg
     1261 agccagccgc cttcatcgag ctcgttccag gcgtagaggg tgactagtgg gcttgacggg
     1321 acgcgatccg ggtgcgtggc gacccagtcg agcgctgcag caacgtactc gcggagggcg
     1381 tcgggcgtgc ggccctcgaa ccagcggtca taggtgtcca tgaagcccat gcgcggacgc
     1441 ggatcccagc ctgtggtgac attggggata tagggaagcc cagccggcgc gctatcccag
     1501 gtggctcgga cgctgtcgac gagggccgag tatggattcg cccccggttg cccgccgaag
     1561 ttgacatagc tcgatacggc atcgaacccg agggcccgga aacgatcggc ctggccgatg
     1621 gagaagtggt tcgcgacgat gtagaggccc gggaggtccg ccttcgcggc ggccgcgcgt
     1681 acccgcgtga tctggtcgcg aagggcctca tcgccgcccg gtgtgctgtc cagcacgtcg
     1741 gcatagacga acagcgcggg ccggccatcg atgcgggtgt agtcgggatc ggccgcgcag
     1801 ccaagccatt cggcgaccgc cgcgtccagg cggtcagcgg gcaccggttg cgtccacatg
     1861 aggcagaagc ccacggcgtg ccgattgggg gtcctcaggt agaggtcgag ccccgtgtcc
     1921 atcaagtccg acggatggct atcgtcgaac cgccaggcgc cccgctcata ccagaggaag
     1981 acgataaagt cgatgccatg agatgccatg aggccgaggg cctggtccat ggcctcgggt
     2041 gagtcgctcc gccagccgta gagcggctcg cgctcggcga acgcggcgag cgtcgcaacg
     2101 tcgctgatcg gcacgttctg caggccgagt tcgcgggccg tgccggcatt gcccggcgcg
     2161 acgaaaacct tctgcgtgcg cggactttgc gccaggcgcc aggccaaggc atgctcgcgc
     2221 ccgccggaac cgattaccag aatcttcatg ttcgctccag aagcgcgcgc ggatctcgaa
     2281 agccgcgccg gctcacgagt cagtggcgga agtggcggaa ccccgtgaat accatcgcga
     2341 tgtcctgctc gtcggccgcg gcgatgactt cggcatcgcg catgctgccg ccgggctgga
     2401 tcactgccgt ggcgccggcc ttggcgagc
//
LOCUS       DAOSPL010000695         2429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43351_length_2429_cov_3.099832, whole genome shotgun sequence.
ACCESSION   DAOSPL010000695 DAOSPL010000000
VERSION     DAOSPL010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43351_length_2429_cov_3.099832"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..>2429
                     /locus_tag="PLQ54_12505"
     CDS             186..>2429
                     /locus_tag="PLQ54_12505"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOS94130.1"
                     /translation="MEPLQILRQLPLFADVPEPLLEAIARGASWRPVAAGEVVVEAGD
                     PGDALYVINDGTVEILVRTADGIAPTRVATLGPGECFGEMALLTGEPRSATVRALTDT
                     TLLRVASEDFSRLVADVSVYQRLVTLLCSRLRRTDVQLEEAQWAQMALSRHLQGAHAH
                     GDTEIHGSTPASRALRDAIEAAAAASGPVLLEGEAGSGKSLAASLIIAKSARADGPVI
                     SIDCSAAGVDAASEIFGHEAGTLPGARARHLGALELCDNGTVIIAEPHRLPTMVQERL
                     VGALERGVFQRIGGDEELPLNARVIVTRRMEPSAAVPAVDPGIEALFSGGHIRVPPLR
                     DRRRDIPQLAEALIERHARDLRRPKPELDPGAIERLLSYEWPGNVEELDSVLRRAVAL
                     FADDRIEADHILIHLPSISEEGRVDLFRIPFVAKFVGSRWYPLALQLPTAAVLALIVF
                     ECFFGPQTGENIALTLTWPIWWAALPLSFLFLGRIWCTICPFSLLSSVVQRIGCLNLP
                     VPAFFRKTEIWPMTALFVFLTWADEYWHYPDKPMWTGAVLGSVLAGTLLFSFLFERRV
                     WCRYLCPLGGVNGVYSTAALVELRANTDVCAYHCRAHECVSKEPGRACPMLERPLAMD
                     TNRNCNLCMNCVKHCPHGAIHLFLRRPGAEIWQIRTPMLSAGVLCLLLTGTMLTHGLC
                     KYVESQGKHLIDVPPATWLGFTAPAQEGAAWTATYLALLLLSVLAGCIASAVSSSRER
                     KPWSSNLA"
BASE COUNT          347 a          807 c          837 g          438 t
ORIGIN      
        1 ccagcggcca gtggcgcggc tggtggccgc atggatacca gcccgactcc atgccgcccc
       61 tacgcgttcg gggtgcccgt gagcgaggag gctgattccg tggcgccgaa tagcgtgttg
      121 tcggttctgg ctctgccggc cgcttcgcgc cgttgtctac cgtcccgata agggtctggg
      181 agtacatgga acccctacag atcctgcgcc agttgccttt gttcgccgat gtgcctgaac
      241 cgctcctcga agccattgcc agaggcgcgt cttggcggcc ggtcgcggcc ggtgaggtgg
      301 tggttgaggc cggcgacccc ggcgacgcgc tctatgtgat caacgacggc accgtggaga
      361 tactggtgcg gacagccgac gggatcgcgc ccacgcgcgt cgccacactc ggtcccggcg
      421 agtgcttcgg cgagatggcc ctgctgaccg gcgagccgcg gtcggctacg gtgcgcgcgc
      481 ttaccgacac cacgctgctg cgagtggcta gcgaggactt cagccggctg gtggccgacg
      541 tgtcggtcta ccagcggctg gtcactctcc tgtgctcgcg cctgcgccgg accgacgtgc
      601 aactggagga ggcccagtgg gcccagatgg cgctcagccg gcacttgcag ggagcgcacg
      661 cccatggcga taccgagatc catggctcga cgcccgcctc gcgagccttg agagacgcga
      721 tcgaggcggc cgcagcagcc agtggccccg ttctcctcga aggggaagcc ggttctggca
      781 agagcctggc cgcgtcgctc atcatcgcca agagcgcccg cgccgatgga ccggtcatct
      841 cgatcgattg ctcagcggcc ggtgtggatg ccgcgtccga gatcttcggc cacgaggccg
      901 ggacgctgcc cggggcccgg gcgcgccatc tcggggcact ggagctctgc gacaacggca
      961 cggtcattat tgcggagccg catcggttgc ccaccatggt gcaggagcgg ctggtcggcg
     1021 ccctggagcg gggcgtgttc cagcggatcg ggggcgacga ggagctcccc ctgaacgccc
     1081 gagtgatcgt cacccgccgg atggagccat cggcggcggt gcccgcggtg gaccctggca
     1141 tcgaggccct gttctcgggt ggtcacatcc gcgtgcctcc actccgcgac cggcgtcgcg
     1201 acatcccgca gctggccgag gcgctcatcg agcggcacgc gcgggatctg cggcgcccca
     1261 agccagagct cgatcccggt gccatcgagc ggctgctgag ctacgaatgg ccgggaaacg
     1321 tcgaggaact cgatagtgtt ctgcgacggg cggtggcgct gttcgccgat gatcgcatcg
     1381 aggccgatca catactgatt cacttgccga gcattagcga agagggccgg gtagacctgt
     1441 tccgcatccc cttcgtcgcc aagtttgttg gcagcagatg gtatcccctg gccctgcagt
     1501 tgccgacggc cgcggtgctg gctctcatcg tgttcgagtg cttcttcggc ccgcagactg
     1561 gggagaacat tgcgctgacc ttgacgtggc ccatctggtg ggcggccctg ccgctgagct
     1621 tcctcttcct gggccgcatc tggtgtacca tttgtccatt ctcgctcctc agctcggtgg
     1681 tgcagcggat cggatgcctg aacctgccgg tgccggcgtt cttccgcaag accgagatct
     1741 ggcccatgac cgcgctgttc gtgttcctga cgtgggcgga cgagtactgg cattatcccg
     1801 acaagccgat gtggaccggc gccgttctcg gttcggttct ggccggcacg ctgctgttca
     1861 gcttcctgtt cgagcgccgg gtctggtgcc gctacctctg cccgttagga ggcgtcaatg
     1921 gcgtctactc gacggcggct ctggtggagc tgcgcgcgaa caccgacgtc tgcgcctacc
     1981 actgccgcgc gcacgaatgt gtgtcgaagg agccgggccg agcctgcccc atgctcgagc
     2041 gcccgctcgc gatggacacg aaccgcaact gcaacctgtg catgaactgc gtgaagcact
     2101 gcccacatgg ggcgatccac ctgttcctga ggaggcccgg ggccgagatc tggcagatcc
     2161 gcacgcccat gttgtcggcc ggagtgctgt gtctgctcct gacgggtacc atgctcacgc
     2221 acgggctctg caaatacgtg gagtcccagg ggaagcacct catcgacgtg ccaccggcca
     2281 cgtggctcgg tttcacggcc ccggcccagg agggcgcggc ctggacggca acgtacctgg
     2341 cgctgctgct gctgtcggtc ctcgccggct gcatcgcgag cgcggtgtcg tcgtcgcggg
     2401 agcggaagcc gtggtcgtcg aacctggcg
//
LOCUS       DAOSPL010000696         2426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43435_length_2426_cov_2.846057, whole genome shotgun sequence.
ACCESSION   DAOSPL010000696 DAOSPL010000000
VERSION     DAOSPL010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43435_length_2426_cov_2.846057"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PLQ54_12510"
     CDS             complement(<1..462)
                     /locus_tag="PLQ54_12510"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOS94131.1"
                     /translation="MPEDTSGRESTSPSVEAVPYDPSDNGEHDEDGPSEETATFVEHL
                     DELRTRLIRCIVYILVLAGLSFSVLNKLIIDVLLSPVQDIVKSDPVGGLISTAPAEWI
                     FIWVKILLISGVFLAGPLILGEIYGFIAPALTRSEKRYARWAFLFAPLLFAA"
     gene            complement(498..1616)
                     /gene="ugpC"
                     /locus_tag="PLQ54_12515"
     CDS             complement(498..1616)
                     /gene="ugpC"
                     /locus_tag="PLQ54_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOS94132.1"
                     /translation="MASVRLHHLSKRFRDVVAVNDLTLEVKDKEFLVLVGPSGCGKTT
                     ALRMIAGLEEATSGDIYIGDRRVNDDSPKDRDIAMVFQNYALYPHMSVYDNMAFGLKL
                     RKMPKEKIRALVDDAAKMLGLEGLLNRKPKELSGGQRQRVALGRAIVREPQVFLMDEP
                     LSNLDAKLRVQTRAELIKLHRRLGITTVYVTHDQVEAMTMGDRIAVLNAGVMQQCDTP
                     LNLYGSPANRFVAGFIGSPAMNFLEAKLTSENGSTYVDAAGLRLRLPDDRAARVRERG
                     YLGKPVILGIRPEDIHDRELSLIPPDEGNTATVRVDVTEPMGDRVIVYALVNGETILA
                     SLGSATKAKELTDLQVVLDTSRIHLFDCDTECSLLVDA"
     gene            complement(1744..>2426)
                     /locus_tag="PLQ54_12520"
     CDS             complement(1744..>2426)
                     /locus_tag="PLQ54_12520"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOS94133.1"
                     /translation="GVRMVREDGHVLGLKPQFSILDADDVTGILKEASGGTTDMAVIS
                     LGSPVVSDSIRIAGNRFDQAISRHIRRAYGLQIGDPTAEYVKIEIGSAVELQPELELE
                     VRGRDSITGLPRTVVVRSFDIREAILEPVYAIIERVKQLLEQTPPELAADIIERGITM
                     SGGGCLLRGTDRLLSVETGIPVVIADDPMCCVAKGTGAVLEHIDQLRDTRPTEPTPLP
                     GGYVRRAR"
BASE COUNT          435 a          766 c          785 g          440 t
ORIGIN      
        1 ggccgcaaac agcagcggag cgaagagaaa cgcccagcgc gcataccgct tctcagaccg
       61 ggtcagcgcc ggagcgatga acccgtagat ctctcccaga atcagggggc cggcgaggaa
      121 cacgcctgat atcagtagga tcttcaccca gatgaagatc cattcggcag gtgccgtcga
      181 gatgagccca cccacaggat cgctcttcac aatgtcctgc actgggctca ggagcacatc
      241 gatgatgagt ttgttgagga cgctgaatga gagcccggcc aataccagga tgtagacgat
      301 gcagcggatc agccgagtgc ggagttcgtc gagatgctcg acaaaggtgg ccgtctcctc
      361 gctgggaccg tcctcatcgt gctccccatt gtcgctcgga tcgtacggca ccgcctcgac
      421 cgatggcgag gtggactccc gacccgaggt gtcctctggc atcagcgtgt ttgatcccgt
      481 ctcgtgcggc cgagagccta cgcgtcgacg aggagcgagc actcggtgtc gcaatcgaat
      541 aggtgaatgc ggctcgtgtc gaggacgacc tgcaggtctg tgagctcctt ggccttggtg
      601 gccgagccca gggaggccag gatggtctcc ccattgacca gagcgtagac gatcacgcgg
      661 tcgcccatgg gctcagtgac gtccacccgc accgtcgccg tgttgccctc gtccggtgga
      721 atcagtgaca gctctcgatc gtgaatgtcc tcagggcgaa tacccaggat tacgggcttg
      781 cccaggtagc ctcgctccct cacacgcgcg gcgcggtcgt cgggcagccg aagcctcaga
      841 cctgccgcgt ccacataggt ggagccgttc tccgaggtga gcttggcttc caggaagttc
      901 atcgccgggc tcccgatgaa acccgccacg aatcggttgg ccggagagcc atacaggttc
      961 agcggggtgt cgcactgctg catcacgccc gcgttcagca cggcgatccg gtcgcccatg
     1021 gtcatggcct caacctggtc gtgggtaacg tacaccgtgg taatgccgag gcggcgatga
     1081 agcttgatca actccgcgcg ggtctggacg cgcagcttcg cgtccagatt cgagagcggc
     1141 tcgtccatga ggaacacctg tggctcacgg acgatggctc ggccaagtgc cacccgctgc
     1201 cgctgtcctc ccgacaactc cttgggcttg cggttcagca gcccctcgag gccgagcatc
     1261 ttcgctgcat cgtccacgag ggcccttatc ttctccttgg gcatcttgcg gagcttgagc
     1321 ccaaaggcca tgttgtcgta gaccgacatg tggggataga gggcatagtt ctggaagacc
     1381 atggcgatgt ctcggtcctt gggggagtca tcgttgacgc gccgatcacc gatgtatatg
     1441 tcacccgagg tcgcctcttc gagcccggcg atcatgcgca gtgccgtggt cttcccacac
     1501 cctgaggggc caaccagaac cagaaactcc ttgtccttca cctcgagcgt caagtcattg
     1561 acggccacca cgtcgcgaaa ccgcttcgag aggtgatgga gccggacact ggccattcgc
     1621 gaagccccct ggacgaacgc acatgagcag ggaccggggc aagcccagtc cgcagaggtg
     1681 gtccctactt ggcgcggcca ttatagggat gccctgttcg aggtgtcaat tcaggcgggg
     1741 ggctcagcgc gcccggcgga cgtagccgcc ggggagcggc gtgggctcgg tcgggcgcgt
     1801 gtcgcggagc tggtcgatgt gctccaggac ggctccagtc cccttggcga cgcagcacat
     1861 cgggtcgtcg gcaatgacca cggggatgcc cgtctccacg gacagcaggc ggtcagtgcc
     1921 tcgcagcagg catccccctc cactcatggt gatcccgcgc tcaatgatgt ccgcggccag
     1981 ttcgggaggc gtctgctcga ggagctgttt cacgcgctcg atgatggcat agaccggctc
     2041 caggatggcc tcgcggatat cgaacgaacg cacgaccacg gtgcggggca agccggtgat
     2101 gctatcccgg ccacgcacct cgagttcgag ttctggctgg agctcgacgg cggagccgat
     2161 ctcgatcttg acgtactcgg cggtggggtc accgatctgg aggccatagg cccggcggat
     2221 atgccggctg atcgcctggt cgaagcggtt gccggcgatg cggatgctat cactcacgac
     2281 gggcgacccc agcgagatca ccgccatgtc ggtggtgccg cccgaggctt ccttgaggat
     2341 gcccgtgacg tcgtcggcgt cgaggatgct gaactgcggc ttgaggccca gcacatggcc
     2401 gtcctcgcgc accatgcgca cgccca
//
LOCUS       DAOSPL010000697         2424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43466_length_2424_cov_3.235542, whole genome shotgun sequence.
ACCESSION   DAOSPL010000697 DAOSPL010000000
VERSION     DAOSPL010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43466_length_2424_cov_3.235542"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PLQ54_12525"
     CDS             complement(<1..544)
                     /locus_tag="PLQ54_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOS94134.1"
                     /translation="MGRKLACVLGLALVLMAGAAAWADAGAKDTVAPPAKAGMPADVA
                     FDHWAYDAVADMYDAGLLEGYPDGTFKGNNSLTRYEFAMALARTLWTIKTNPEYKGPK
                     GDKGDTGAAGPAGPKGDTGPAGPAGPAGPKGDKGATGPAGPKGEKGAKGDKGDRGPGP
                     TPAEVDEGVKRVFGDMGPQTA"
     gene            complement(906..2132)
                     /locus_tag="PLQ54_12530"
     CDS             complement(906..2132)
                     /locus_tag="PLQ54_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOS94135.1"
                     /translation="MDDTLSMDPSPPPVPDLLPPPRPQRSGCALVAIAVISGAIAGLA
                     GCLVALAYFMPQIQNRVGSQGAPALAPLRPGKTVTAQTDLTIEGGDSEAIKRAIAKVM
                     PAVVHVTQAIPRDVPIRTPQGGRDVGAGSGFIIDGKKGFVVTNAHVLANPGLPTEPEL
                     YVKLAGQDDSQRILARRLAVDPYHDLAVLKIDTGGKELPQLQFGDSEKLALGDWVIAL
                     GSPFGLAETATVGIVSAKHRRLAIADKTYDDLLQTDAAINMGNSGGPLVNLNGDVVGV
                     NSVIQSPTGTSAGVGFAIASSRARTYIEDMMSRGYLGVVMGPYEQKPGTAAAPDGHMC
                     EVRDVLPKGPADAAGIKVGDVILRIDSTDIAGMEEVVNLVGSKKPGTVLEIVVFRPSE
                     DRQVRLKATVGDRTEY"
     gene            complement(2137..>2424)
                     /gene="ribH"
                     /locus_tag="PLQ54_12535"
     CDS             complement(2137..>2424)
                     /gene="ribH"
                     /locus_tag="PLQ54_12535"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOS94136.1"
                     /translation="RRYSAVLCLGAVIRGDTPHFDYVANESARGIADISAQLGVPVIY
                     GIMTADNLEQAIERAGTRMGNKGAEAAFAGIEVANLLARIDSALGGPDKVE"
BASE COUNT          373 a          829 c          772 g          450 t
ORIGIN      
        1 ccgcagtctg aggacccatg tcgccgaaga ccctcttgac accctcgtcg acctcagcgg
       61 gcgtcgggcc agggccacga tcgcccttgt cgcccttcgc acccttctcg cctttggggc
      121 cggcagggcc cgttgcgccc ttgtcgccct tggggccagc aggacccgcg ggaccggctg
      181 ggccagtatc acccttgggg ccagcgggac cggcagcgcc ggtatcgccc ttgtcgccct
      241 tggggccctt gtactcgggg ttggtcttga tggtccacag cgtgcgcgcc agggccatcg
      301 cgaactcgta ccgagtcagg ctgttattgc ccttgaaggt cccgtcgggg tagccctcaa
      361 gcagcccagc gtcgtacatg tcggccactg cgtcataggc ccagtggtcg aaggccacgt
      421 cggccggcat gccagccttg gcgggcggag ctaccgtatc ctttgcgccc gcatcggccc
      481 atgccgcggc accggccatg agcacgagcg ccaagcccag aacacatgcg agcttgcgtc
      541 ccatttgcgt ttttcctcct acgatatgct tcggtgtgac ccacagtaga agcacggcgc
      601 tatcgcaggc ggagagacac aatgcctggg ctggcgagcc cgtcgcatgc agcgtgagtt
      661 gccgtgtttc ctcatggctg cggcgcggcg gtacttcacc cgcttcggac gacgtctctc
      721 ctcttggcgc ctcccttcct gcgcaagatt gtgctcctac cccaggggcc aaaccctccg
      781 ctccgttcac ggggttcaac cccgttcgcg gattcccctg caaggtcccg aaacaagatt
      841 ccggcagatt gtagcggcac ccgtcaaccc tgtcaaaccg aactcgacaa taacactccc
      901 acctgttagt actcggtccg atctcccacg gtcgctttca gcctcacctg gcggtcctcc
      961 gatggccgga acaccacgat ctccagcacg gtgcccggct tcttggatcc caccaggttc
     1021 acgacctcct ccatgcccgc aatgtcggtg gagtcgatac ggaggatcac gtcaccgacc
     1081 ttgatccccg cagcgtcggc cggtcccttg gggagtacgt ctcgcacctc gcacatgtgc
     1141 ccgtcgggag ccgcggccgt gcccggcttc tgctcgtagg ggcccatcac gacgccgagg
     1201 taaccccgcg acatcatgtc ttcgatgtag gtacgggccc ggctcgatgc aatggcgaat
     1261 cccactccgg cgctggtgcc cgtgggcgac tggatcacgc tgttgacccc aaccacatcg
     1321 ccattgaggt tgaccagggg gccaccgctg ttgcccatgt tgatggctgc gtcggtctgg
     1381 aggaggtcgt cgtaggtctt atcggcgatg gcaaggcgcc ggtgtttggc gctgacgatc
     1441 cccaccgtcg ccgtctcggc cagtccaaag ggactgccga gcgcgatgac ccagtcaccc
     1501 agggccagct tctcggagtc gccaaactgg agctgaggca gctctttgcc cccggtgtca
     1561 atcttgagca ccgccaggtc gtggtacggg tccactgcca gtcggcgggc caggatccgt
     1621 tggctgtcat cctgcccggc gagcttcaca tagagctcgg gctcggtcgg caatccgggg
     1681 ttggcaagta cgtgggcatt ggtgacgacg aaccccttct tgccatcgat gatgaacccg
     1741 ctcccggcgc ccacgtccct gccgccctgc ggagtgcgga tgggcacatc ccgcgggatg
     1801 gcctgggtca cgtgcaccac tgcaggcatg accttggcga tggcccgctt gatggcctcg
     1861 gagtcgcctc cctcgatggt gaggtcggtc tgcgcggtga ccgtcttgcc ggggcgtagt
     1921 ggggcgaggg ccggtgcgcc ctgcgagccc acccgattct ggatctgcgg catgaagtag
     1981 gccagggcaa cgaggcaccc ggccaacccc gcgatggcgc cggatatcac ggcgatagcc
     2041 acaagggcgc aacccgatcg ctggggccgc ggcggtggga gcaggtcggg caccggcggg
     2101 ggagagggat ccattgagag tgtgtcgtcc atgcggctat tccaccttat cgggtccgcc
     2161 gagcgcggag tcgatgcggg ccagaaggtt ggcgacctcg atcccggcga atgccgcctc
     2221 ggcccccttg ttgcccatac gcgtgccggc acgctcgatg gcctgctcga ggttgtcggc
     2281 cgtcatgatc ccatagatca ccggtacccc gagctgtgcg ctgatgtccg cgatcccccg
     2341 cgccgactcg ttggcgacat agtcgaagtg tggtgtatcg ccgcggatga cggcgccgag
     2401 gcacagcacg gcgctatagc gccg
//
LOCUS       DAOSPL010000698         2423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43500_length_2423_cov_2.885557, whole genome shotgun sequence.
ACCESSION   DAOSPL010000698 DAOSPL010000000
VERSION     DAOSPL010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43500_length_2423_cov_2.885557"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..521)
                     /gene="hisI"
                     /locus_tag="PLQ54_12540"
     CDS             complement(111..521)
                     /gene="hisI"
                     /locus_tag="PLQ54_12540"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOS94137.1"
                     /translation="MSASDSAGGTGSVIESLKFNEQGLIPAIVQDDENGQVLMMAWMN
                     AEALRRTIESGQTCFWSRSRQEYWVKGLTSGHTQKVRSLHIDCDRDVVLVRVQQVGAA
                     CHEGYRTCFYRRIDGTRPDAVCLTTTEERVAQPS"
     gene            complement(511..1266)
                     /gene="hisF"
                     /locus_tag="PLQ54_12545"
     CDS             complement(511..1266)
                     /gene="hisF"
                     /locus_tag="PLQ54_12545"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003349403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOS94138.1"
                     /translation="MLAKRIIPCLDVKDGRVVKGVQFVDLRDAGDPVEQARVYDAEEA
                     DELVFLDITASHEKRDTMIDVVRRVAETIAIPFSVGGGIHELGNIRDIVAAGADKVSL
                     NTAAVLTPELISEGAEHFGRQCICVAMDPKRMPDGRWIVHTHGGRRPSAWDVRDWAPR
                     VEELGAGEIMLTSMDRDGTKAGYDIEMTRTVAESVSVPVIASGGAGTVEHLLEALTEG
                     KAHAVLAASIFHFREISVREAKAYLRERGVLVR"
     gene            complement(1260..2009)
                     /gene="hisA"
                     /locus_tag="PLQ54_12550"
     CDS             complement(1260..2009)
                     /gene="hisA"
                     /locus_tag="PLQ54_12550"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOS94139.1"
                     /translation="MIVIPSIDIRGGQVVRLRYGDPDQQTVYERDPAATARYFEECGA
                     ELIHVVDLDGAFGGEPRARDIIRAIAAAVSVPIEVGGGIRSMAVIEGHLSLGVKRVVI
                     GTAGIEDPAFLVEAVERFGPETVIGGLDARDGRVALRGWVDTSSLTAGEVGRRMHDAG
                     VRTVVHTDIGRDGVMTGPNVDASLALAATTGLRVIVSGGVSSLADIRRVCEAARMPTG
                     APPALVGCITGRAIYEGAVDLAAAVELSRAC"
     gene            complement(2006..>2423)
                     /gene="hisH"
                     /locus_tag="PLQ54_12555"
     CDS             complement(2006..>2423)
                     /gene="hisH"
                     /locus_tag="PLQ54_12555"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOS94140.1"
                     /translation="SGKPFLGICLGLQVLFESSEEAPGVRGLGVLEGTVKRFAPGLHV
                     PHMGWNQVRLVGDTPLLGGIADGDFFYFVHSYYPVPDDPDARATVTEYGIEFTSSVQR
                     GNLFASQFHPEKSQAKGLTILRNFVSLVGGQAGTAP"
BASE COUNT          406 a          812 c          829 g          376 t
ORIGIN      
        1 gcgagcctgg tgaacgaggt cggccggcgt ctcgggcggt tgcgggtcct gggcggcggc
       61 aagggtgagg cacaggagca ccagcaggcc gaaccagagg cgccaggggt tcaactgggc
      121 tgggccacgc gttcctcggt ggtcgtgagg cagacggcgt cgggccgggt accatcgatc
      181 cggcggtaga agcacgttcg gtagccctcg tggcacgccg cacccacctg ctggacccga
      241 accaggacca cgtcgcggtc gcagtcgatg tggagactcc tcaccttctg cgtgtggccc
      301 gatgtgagac ccttgaccca gtactcctgg cgcgaacggc tccagaagca cgtctggccc
      361 gactcgatgg ttcggcgcag cgcctctgca ttcatccagg ccatcatcag aacctggccg
      421 ttctcatcat cctgcacgat ggccgggatg aggccctgtt cgttgaactt cagactctcg
      481 atgacgctgc cggttccacc ggcgctgtcg ctagcggaca agcacgccac gctccctcag
      541 ataggctttg gcctcgcgga cgctgatctc gcggaagtgg aagatcgagg cggccaacac
      601 ggcatgcgct ttgccctcgg tgagcgcttc gagcaagtgc tcgaccgtgc cggcgccacc
      661 ggaggcgatc acgggcacgc tgaccgactc ggcgacggtg cgggtcatct cgatatcgta
      721 ccccgccttg gtcccatcgc ggtccatact ggtcagcatg atctcgccgg cgcccagctc
      781 ctcgacccgg ggcgcccagt cgcggacgtc ccaggccgaa ggccgcctgc caccgtgggt
      841 gtggacgatc cagcgcccat cgggcatgcg cttggggtcc atggccacgc agatgcactg
      901 gcggccgaag tgctcggcgc cctcggagat aagctccggg gtcaggaccg ccgccgtgtt
      961 aagcgacacc ttgtcggcgc ccgcggccac gatgtcccgg atgttgccca gctcgtgaat
     1021 gcccccgccc acgctgaagg ggatggcgat ggtctcggcc acccgacgga cgacatcgat
     1081 catggtgtca cgcttctcgt gggaggccgt gatgtcgagg aacaccagtt cgtcggcttc
     1141 ctcggcatcg tagacgcgcg cttgctcgac ggggtcgccg gcgtcgcgga gatcgacgaa
     1201 ctggacgcct ttgacgacgc ggccgtcctt cacatcgagg cacgggatga tgcgcttggc
     1261 tagcacgctc tgctcaactc cacggcagcg gccaagtcga ccgcgccctc gtagatggct
     1321 cgtccggtaa tacagccgac cagcgcaggt ggggcgcctg tgggcatccg agcggcctcg
     1381 cacacgcgcc ggatgtcggc cagtgaggag acaccaccgc tgacgatgac ccgaaggccc
     1441 gtggtggcgg ccagggcgag cgaggcgtcg acgttgggac cggtcatgac gccatcgcgc
     1501 ccgatgtccg tgtggacgac ggtgcggacg cccgcatcgt gcatccgccg gccgacctcg
     1561 ccagcggtca gactcgaggt gtccacccaa ccgcgaagcg cgacacggcc gtcccgggcg
     1621 tccaggccgc cgatgaccgt ctccggcccg aaccgttcga cggcctcgac gaggaaggcg
     1681 ggatcctcga tgcccgcggt gccgatgacc acgcgcttca cgcccaggga gaggtgcccc
     1741 tcgatgaccg ccatcgagcg gatgcctcca cccacctcga tgggcacgga gacagctgcg
     1801 gcgatggcgc ggatgatgtc gcgagcccgg ggctcgccac caaaggcgcc gtcgaggtcc
     1861 accacgtgga tgagttccgc cccacactcc tcaaagtagc gggccgtggc ggcggggtcg
     1921 cgctcgtaga ccgtctgctg atcggggtcg ccgtagcgca ggcggacgac ctggccgccg
     1981 cggatgtcga tggatgggat gacgatcatg gagcggtccc cgcctgcccg ccaaccagac
     2041 tgacgaagtt gcgcagaatg gtcaggccct tggcctggct cttctcgggg tggaactggc
     2101 tcgcgaacag gttaccgcgc tgcaccgagg aggtgaactc gatgccgtac tcggtgaccg
     2161 tagcgcgggc gtccgggtca tcgggcaccg ggtagtagct gtggacgaag tagaagaagt
     2221 caccgtccgc gatgccaccg agcaggggcg tgtcgccgac caatcgcacc tggttccagc
     2281 ccatatgggg gacgtgcagc ccaggcgcga aacgcttcac ggtaccctca agcacaccga
     2341 ggccgcgaac accgggggct tcctcactgg actcgaacag cacctggaga ccgaggcaga
     2401 tgccgaggaa gggttttccg gag
//
LOCUS       DAOSPL010000699         2420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43581_length_2420_cov_2.213953, whole genome shotgun sequence.
ACCESSION   DAOSPL010000699 DAOSPL010000000
VERSION     DAOSPL010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43581_length_2420_cov_2.213953"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..>2420
                     /locus_tag="PLQ54_12560"
     CDS             108..>2420
                     /locus_tag="PLQ54_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HOS94141.1"
                     /translation="MTERVRQLRQQSLDTVPYISTERAELVTEAYERFGACPAPILRA
                     RVFAHLMEHKAVHIGEGELIVGERGPAPKATPTFPELCCHSLQDLDIIAGREKIRFGV
                     SPEARRTYEERIIPFWRGRTMRERIFGQMTPEWLAAYEAGIFTEFMEQRAPGHTVLDD
                     KVYHRGLLDFRAEIHRVAADAPNDGLTAMAITCDALVRYAERHAERAEELAAVEPDAA
                     RRAELERIARVCRHVPAHAPRDFWEALQMYWFVHLGVVTELNTWDSFNPGHLDRHLEP
                     FFQAGLAKGTLTRESAKELLECFWVKFNNQPAPPKVGVTAAESGTYTDFANINNGGLH
                     VDGTDAVGAVTYLILDVIDEMRLLQPSSNIQLSACSPDAFLRRACEIIRQGWGQPSVF
                     NADVVVREMLRQGKTVEDARAGGTSGCVETGAFGKEAYILTGYFNLVKVLEITLNGGL
                     DPRTGKRIGADTGDPRGFTTFDELFDAFRRQIHHFVDTKIAGNAVIERLYANDMPAPF
                     LSVLTDDCIARGRDYHDGGARYNTTYIMATGTGTLTDSLSAIKHHIYGGGELQWDELL
                     AALRDDFVGHEALRQMLWHRTPRFGQDDDEADALMRRHFDAFHDAVDGRPNGRGGCYH
                     VNYLSTTCHVYFGSVTGATPDGRRAGTPLSDGASPVQGTSTRGPTAVIRSLAKMDHER
                     TGGTLLNLKFTPRLLGGDEGLDRLGQLIRTYFDLGGHHVQFNVVTAETLRAARAHPEQ
                     YRDLIVRVAGYSDYFCDLTPALQEEIIARTE"
BASE COUNT          415 a          869 c          750 g          386 t
ORIGIN      
        1 tccgggcctg ccatccgcca tcggctatct gcgttctgcg ttgacaccct tcccggacac
       61 cgctatacat ccagtgtctt cccaccgcgc tccgatcgag ggtccccatg accgaacgcg
      121 tccgccaact ccgccagcag agcctcgaca ccgtgcccta catctcgacg gaacgggccg
      181 agctggtcac ggaggcctat gagcgcttcg gtgcctgccc cgctccgatc ctgcgggctc
      241 gcgtgttcgc tcacctcatg gagcacaagg ccgtacacat cggtgagggc gagctcatcg
      301 tcggcgagcg cgggcctgct cccaaagcga cgccgacatt ccccgagctg tgttgccatt
      361 cgctgcagga tctcgacatc atcgcgggcc gcgagaagat ccgcttcggt gtgagccccg
      421 aggcccgccg aacctacgag gagcgtatca tacccttctg gcgcggccgc accatgcgcg
      481 agcgcatctt cggccagatg acccctgagt ggcttgccgc ctacgaggcc ggcatcttca
      541 ccgagttcat ggagcagcgt gctcccggcc acaccgtcct cgacgataag gtctaccacc
      601 gcgggctact ggacttccgc gccgagatcc accgggtggc cgcggacgcg ccgaatgacg
      661 ggctcaccgc catggcgatc acgtgtgatg ctctggtccg ctacgccgag cgtcacgcgg
      721 agcgcgccga ggagctggct gctgtcgagc ccgacgccgc gcgccgggcc gagctcgagc
      781 gcatcgcccg cgtctgccgc cacgtgccgg cccatgcgcc gagggacttc tgggaagccc
      841 tccagatgta ctggttcgtc cacctgggcg tcgtgaccga gctcaacacg tgggactcct
      901 tcaaccccgg ccatctcgat cgccacctgg agcccttctt ccaggcgggg ctcgccaagg
      961 gcacgctcac gcgcgagtcc gccaaggagc tgctcgagtg cttctgggtg aagttcaaca
     1021 accagcccgc gccgccgaag gtgggcgtga cggcagccga gagcggcacc tacaccgact
     1081 tcgccaacat caacaacggc gggctgcatg tcgacggaac cgacgccgtc ggcgccgtga
     1141 cctatctgat cctggacgtc attgacgaga tgcgactcct gcagccgtcg tccaatatcc
     1201 agctcagtgc ttgcagcccg gatgcattcc tcaggcgcgc ttgcgagatc attcgccagg
     1261 gctggggcca gccatcggtg ttcaacgcgg acgtggtcgt tcgggagatg ctccgccagg
     1321 ggaagaccgt cgaggatgcc cgggccggcg gcacgtccgg ctgcgtcgag accggggcat
     1381 tcggcaagga agcctacatc ctcacgggct acttcaacct ggtgaaggtc ctcgagatca
     1441 cgctgaacgg cgggctcgat ccgcgaacgg gtaagcgcat cggcgccgac acgggcgacc
     1501 cgcggggctt cacgaccttc gacgagctgt tcgatgcctt tcgcaggcag atccaccact
     1561 tcgtcgacac caagatcgca gggaacgcgg taatcgagcg gctctacgcc aacgacatgc
     1621 ccgcgccatt cctctccgtg ctgaccgacg actgcatcgc tcgcggccgc gactaccacg
     1681 acgggggcgc ccgctacaac acgacctaca tcatggccac gggcacgggt acactcacgg
     1741 actcgctctc ggccatcaag caccacatct acggcggcgg ggagctccaa tgggatgagt
     1801 tgctggcggc gctgcgggac gatttcgtgg gccatgaagc gctgcggcag atgctgtggc
     1861 accgtacgcc ccggttcgga caggacgacg acgaggccga cgcgctgatg cgacgccact
     1921 ttgatgcatt ccacgacgcc gttgatggcc ggcccaacgg acgcggcggg tgctatcacg
     1981 tcaactacct ctcgacgacc tgccacgtct acttcggctc ggtaaccggc gccacgcccg
     2041 atgggcgccg cgcgggcacc ccgctctccg atggcgcctc acccgtccag ggcacgtcca
     2101 cgagaggccc cacggcggtc atccggtccc tggcgaagat ggaccatgag cgcaccgggg
     2161 gaacgctact gaacctcaag ttcacgccgc gactgctcgg gggtgacgag ggcctcgacc
     2221 gcttgggcca gctcatccgc acctacttcg acctcggcgg ccaccatgtc cagttcaacg
     2281 tggtcacggc cgagacgttg cgggccgctc gagcccaccc ggagcagtac cgcgacctga
     2341 tcgtccgcgt ggcgggctac tccgactact tctgcgacct caccccggcc ctgcaggaag
     2401 agatcatcgc ccgcaccgag
//
LOCUS       DAOSPL010000700         2417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43648_length_2417_cov_2.987722, whole genome shotgun sequence.
ACCESSION   DAOSPL010000700 DAOSPL010000000
VERSION     DAOSPL010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43648_length_2417_cov_2.987722"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2406)
                     /locus_tag="PLQ54_12565"
     CDS             complement(<1..2406)
                     /locus_tag="PLQ54_12565"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOS94142.1"
                     /translation="MVKKLSEADGWAPVAGGRGGRARRRGPMVLYVSPLKALNYDIER
                     NLRRPLRGIASAAGRLGLSLPAIDVGVRTGDTPDRERQRMVRRPPHILITTPESLHLM
                     LTGSAREILRDVEYVIVDEIHSVCREKRGVFLSLVLERLTHNIGRDPVRIGLSATMRP
                     LDLVAAYLGGMATHGDGPPAPRPVEIVNAGQRRELDVLVEWVAEARLRDDPGSIWDSV
                     HDRLYELVCAHKSTIVFVNNRGRAERVASALNERAGRVIAYPHHGSVAPGVRREIEEM
                     LKRGKVPAVVATGTLELGIDMGDVDLVCQVESPLTVSRGLQRVGRAGHLMSAVSKGRI
                     LPKHEGDLLEAVVVAREMLDAQVEPTWIPSNCLDVLAQQIVAMVAGEDWDLSELYRVV
                     RRSYCYRDLGFDTFRAVVEMVAGRYECPEWTDLRPRISLDPDRGRVQALPGARNVAVV
                     QGGTIPDTGEYRVCLQGSSSAIGELEEEFVYESRPGDVFLLGTAAWRIRRIEADRVVV
                     SPAGSGPGRMPFWKGEQIPRPAELGLRVGRFVRELCDRAREASTHEDVIRWLRAEYPV
                     DDAGARSLYRYVYEQHVRGPGAPTDRRIVLEQFCDNQRKRQVVLLTLRGRRLHHTLRI
                     AITGYLRREFGLEVNSAHTDDGLVLQLPDLTTVLPLDLLTRISPDEVLDLVLDELAAS
                     ALFGLRFRHNAARALLLPGAKPGKRTPLWLQRLKAKDLLQIVKDYDGFPVIVETYREC
                     MEEHLDVAGCQELLADIRAGRLEVVAVNAPFPSPFASGLIMNLTAAYVYDDDKPHGLS
                     QQAA"
BASE COUNT          436 a          830 c          765 g          386 t
ORIGIN      
        1 ggcagcctgc tggctgagcc cgtggggttt gtcatcgtcg tacacatacg ctgccgtgag
       61 gttcatgatc aaaccggaag caaacggcga cgggaatggc gcattgaccg ccaccacctc
      121 gagtcggccg gcacggatgt ccgccagcag ctcctggcat ccggcaacgt cgaggtgttc
      181 ctccatgcac tcgcgatagg tctcgacgat gaccgggaac ccgtcgtagt ccttcacaat
      241 ctggagcagg tccttggcct tgagccgctg cagccacagc ggcgttcgct tgccgggctt
      301 ggcgcccgga aggagcaggg cgcgcgccgc attgtgccgg aaccgaagac cgaacaacgc
      361 cgaagcggcc agttcgtcca gcacaaggtc gaggacctcg tcgggactga tccgtgtcaa
      421 caagtcaagc gggagcaccg tagtcaggtc gggaagctgc aacacgaggc catcgtcggt
      481 gtgcgctgag ttgacctcca gcccaaactc gcgccgaagg taccccgtga tggcgatccg
      541 tagcgtgtga tgaagccgtc gcccccggag ggtcagcaac accacttggc gcttgcgctg
      601 gttgtcgcag aactgctcga ggacgatacg ccgatccgtg ggggccccag gtccccgaac
      661 gtgttgttcg tacacgtagc gatacagact ccgtgccccg gcgtcatcga ccgggtactc
      721 ggcccgaagc caccggatca cgtcttcatg ggtcgatgcc tcgcgggccc ggtcacacaa
      781 ctcgcggacg aatcggccca ctctgaggcc gagctccgcg gggcgaggga tctgctcgcc
      841 cttccagaat ggcatccggc ctggcccgga gccagccgga gacaccacca cacggtcggc
      901 ctcgatcctg cggatgcgcc acgcggccgt gccgagcagg aacacatcgc ccggtcgact
      961 ctcgtagacg aactcctcct ccagctcgcc gatggctgat gaagagccct ggaggcacac
     1021 acggtactcc cccgtgtccg ggatggtgcc gccctggacc acggcgacgt ttcgggctcc
     1081 tgggagcgcc tggacccggc cgcggtcggg gtccagcgag atccgcggcc tcagatcggt
     1141 ccattcgggg cactcatagc ggccggcgac catctcgacg acagcgcgaa aggtgtcgaa
     1201 acccaggtca cgatagcagt acgagcgccg gaccacccgg tagagctcgc tgaggtccca
     1261 gtcctcaccg gccaccatgg ccacgatctg ctgggccagc acatccaggc agttgctcgg
     1321 aatccaagtg ggctccacct gggcgtcgag catctcgcgg gccacgacca ccgcctcgag
     1381 caggtcgccc tcgtgcttgg ggaggattcg ccccttcgac acggcagaca tgaggtgccc
     1441 ggccctcccc acgcgttgca ggccccgcga gacggtcagg ggcgactcga cctggcacac
     1501 cagatcgaca tcacccatgt caatgcccag ctccagcgta cccgtagcga ccaccgcggg
     1561 caccttcccg cgcttgagca tctcctcaat ctcacgccgc acgccgggag cgacactccc
     1621 atggtgtgga taggcgatca cgcgtccggc cctctcgttc agtgcactgg cgacccgctc
     1681 ggcccgaccg cgattattga cgaaaacaat ggtgctcttg tgggcacaca cgagctcata
     1741 gagccgatcg tggaccgagt cccatatcga acccggatca tcgcgcaggc gtgcctctgc
     1801 cacccactcg acaagcacgt ccagctcgcg gcgctgcccg gcgttgacga tctcaaccgg
     1861 ccggggagca ggaggtccgt ctccgtgggt tgccatgccc ccgagatacg cggccaccag
     1921 atccagcggc cgcatggtgg ccgacaggcc gatgcgtacc gggtctcgtc cgatgttatg
     1981 ggtgagtcgc tctagcacca gcgacaggaa gacgccgcgc ttctcccggc acaccgagtg
     2041 gatctcgtca acgatcacat actcaacatc gcggaggatc tccctggccg agccagtgag
     2101 catcaggtgg agggactcgg gggttgtgat caggatgtga ggcggccgcc gcaccatgcg
     2161 ctgacgctcg cggtccggtg tgtctccagt gcgaacgccc acgtcgatgg cggggaggga
     2221 cagccccagt cgtccggcgg ccgacgcgat gccgcgcagc gggcggcgga ggttgcgctc
     2281 gatgtcatag ttgagtgctt tcagaggcga gacatagagc accatcggac cgcgccggcg
     2341 tgctcgacca cctctccccc ctgcgacggg ggcccacccg tcggcctccg agagtttctt
     2401 aaccagttca tcaatgc
//
LOCUS       DAOSPL010000701         2416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43676_length_2416_cov_3.060991, whole genome shotgun sequence.
ACCESSION   DAOSPL010000701 DAOSPL010000000
VERSION     DAOSPL010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43676_length_2416_cov_3.060991"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(667..1965)
                     /locus_tag="PLQ54_12570"
     CDS             complement(667..1965)
                     /locus_tag="PLQ54_12570"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOS94143.1"
                     /translation="MCRVACAVVCCLVLCAPASLLAPASAADAVSEWSVQIVQPTAGT
                     TLSAVARVSVCARPSEQAPDRLFVGLEGGPWVDMKPGLGRGQWVADLDTTLVPNGKVR
                     LSCVGLASWSKKPKAKADLIVGVNNPLHHYFGDIHAHSSYSDGVLLPHDAYAYARDVS
                     KLDFFCLTDHMELVTEAEWADMRKEAWAANQEGRFVTLEGLEWTKKEGHACVYEGAGR
                     LLPPDIAGFYDTVSRQGALGMFNHPGQGDTVWNGLAFDLFADQVMQLMEVRTDDELTA
                     YVRALKAGWHLAAAGTDDTHGPDWGAGFAWTGIVAPSLTRTNVMWALRERHCYMTRDR
                     NCRLLFTANGTPMGTIIAEPVEHLSLTVSVEDPDATDGIASIELYEDGMVVASTTPEG
                     PKCEWTSRPTPSRGSHFYFVRVKQKDDDALVSAPVWVTVR"
     gene            complement(2043..>2416)
                     /locus_tag="PLQ54_12575"
     CDS             complement(2043..>2416)
                     /locus_tag="PLQ54_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94144.1"
                     /translation="EFTGFFFSRISSVKPAVMGLGWGEVEAFNADTGAAIWSWKAPMI
                     GGTINDVTMTADDRAYYIQTKDGIIGFDVATGMPVLEYPGEFFFCHWMSQRNGTLYFH
                     VSPPTNGYVAAIPLTGAQAAQ"
BASE COUNT          379 a          829 c          795 g          413 t
ORIGIN      
        1 cagcccatcg ggacccggag cgggccggga gtcccgcgat ccaacgctcc gcagtgcccc
       61 gacccggcct ccggccactt cttccgtcac actcacgcgg cttgggcact cacgcccagt
      121 ctccgagcca cctcgctact ggacagccgg cctcgaacag ccgcactgct tccatgcgac
      181 gggcttccag cgccaccgcc gtcttctgtg ggtctttcgg tagcttggcc atgcacacat
      241 gcaccagaca ggcccactcc gtgccgtcat tcccaagacg ggaatgtcat cagtccgggc
      301 cgcatctgtg cctcctctgg tgctccttgg ccccacgagc actgctgtcg atgcccgcga
      361 tatctggtcg taccacgtcc tcgcgatcag gtcgatgatc cgctggatcg gaccaggccc
      421 cggaggcggc gggggctgcg gaggctggat ccagtcgtag cccggcggca tctcgtctga
      481 cctgaggagg ctattcccgc ggaccggccc ggtgccaccg ggcaccggat tcacactcag
      541 ccacgagccg gtgctcggcc ggagccatcg ggcgacgcag acatccgcct ccgggtggcg
      601 gcgtgatccc gtccatcccg gctgccgagt tccggcgcgg ccgaggccac cgctggcgct
      661 cccgtcctac ctcaccgtca cccacacggg cgctgagacg agggcgtcgt cgtccttctg
      721 cttcacgcgg acgaagtaga agtggcttcc cctgctgggc gtcggccggc tggtccactc
      781 gcacttgggg ccctccgggg tggtggaggc cacgaccatg ccgtcctcgt acagctcgat
      841 cgaggcaatc ccatcggtcg cgtcgggatc ctcgacgctc acggtgagcg atagatgctc
      901 aaccggctcg gcgatgatgg tgcccatcgg ggtgccgttg gccgtgaaca gcagtcggca
      961 gttgcggtcg cgggtcatgt agcagtggcg ctcgcgcagc gcccacatca cattcgtccg
     1021 ggtgagcgag ggggccacga tgccggtcca ggcgaatccg gcgccccagt cgggaccatg
     1081 cgtgtcgtcg gtgcctgcgg ccgccaggtg ccagcctgcc ttgagcgcgc ggacgtaggc
     1141 cgtcagttcg tcatcagtcc gtacttccat cagctgcatg acctggtcgg cgaacaggtc
     1201 gaaggccaga ccgttccaca ccgtgtctcc ctggcccgga tggttgaaca tacccagggc
     1261 gccctggcgc gacacggtgt cgtagaagcc agcgatgtcg ggcggcagga gacgcccggc
     1321 gccctcgtag acgcacgcgt ggccctcctt cttcgtccac tccagccctt ccagcgtcac
     1381 gaatcgccct tcctggttcg ccgcccacgc ctccttgcgc atgtcagccc actcggcctc
     1441 ggtgaccagc tccatgtggt cggtcaggca gaagaagtcc agtttcgaca cgtcgcgcgc
     1501 gtacgcgtag gcgtcgtgcg gcagcagcac gccgtcggag taggagctgt gggcatggat
     1561 gtcgccgaaa tagtggtgca gggggttgtt gaccccgacg atcaggtccg ctttggcttt
     1621 gggcttcttc gaccagctcg cgagcccgac gcacgagagc ctcaccttgc cgttgggtac
     1681 gagggtggtg tcgaggtcgg ccacccactg gccgcggccg aggccgggtt tcatgtcgac
     1741 ccacgggccg ccctccaggc ccacgaagag ccgatcgggc gcttgttccg acggacgagc
     1801 gcagacgctg acccgggcca cggccgagag tgtggtcccc gctgtgggtt ggacgatctg
     1861 cacagaccac tcggagactg cgtcggcggc gctagccggc gcgagaaggc tggccggcgc
     1921 gcacagaacg aggcagcaca caacggcaca ggcaacacgg cacattgaaa cacactcctc
     1981 cggcaaggtc acgaggagat atgcgtcagc cgcctgccgt tctcctgtgt gggccgcagg
     2041 gcctactgag cggcctgggc ccccgtcagc ggtatggcgg ccacgtagcc gttggtcggt
     2101 ggcgagacgt ggaagtagag cgtcccgttc cgctggctca tccagtgaca gaagaagaac
     2161 tctccggggt actcgaggac tggcatgccc gtggcgacat cgaagccgat gatgccgtct
     2221 ttggtctgga tgtagtaggc ccggtcgtcg gcggtcatgg tcacgtcatt gatggtgcca
     2281 ccgatcatgg gcgccttcca gctccagatg gcggccccgg tgtcggcgtt gaaggcctcg
     2341 acctcgcccc agccgagccc catcactgcg ggcttcacgc tcgatatgcg ggagaagaag
     2401 aagccggtaa actcgg
//
LOCUS       DAOSPL010000702         2411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43808_length_2411_cov_2.830221, whole genome shotgun sequence.
ACCESSION   DAOSPL010000702 DAOSPL010000000
VERSION     DAOSPL010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43808_length_2411_cov_2.830221"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..662)
                     /locus_tag="PLQ54_12580"
     CDS             complement(273..662)
                     /locus_tag="PLQ54_12580"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOS94145.1"
                     /translation="MLRSALVLAMAFVVCVGAGPGSAAAITVVVDGKVIKTPAAPFER
                     NQTVYVPISPVAKALGWKVEYNAEGKRVVLCKGSVCKSIKVGPGKNDAVTVKGSTFAP
                     YGTLGKVLNAQAAYNSKAARVTFESTA"
     gene            complement(683..2068)
                     /locus_tag="PLQ54_12585"
     CDS             complement(683..2068)
                     /locus_tag="PLQ54_12585"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOS94146.1"
                     /translation="MRADAHRFLVVDDDPDIQQVLVDLLFSLGYTADAVGLGRDAIDR
                     VRAAEYDLVLLDQALPDIPGADVLRQIKHISPNTEVIIVTGQSSLDSAIAAVRFDAFD
                     YLEKPFGVGVLAEAVEKAIHRRRGRLEEHHRLEALVRQHELERVGDTPTRAASDRALW
                     ESVVRDCPDALVAAGTDRVIRLANDAAVRLIGLPASEMVGRPIESLIELGEYEDLVWG
                     ALDTRDSLTGFETFIVNRCTDRRIAVEINWSVLRDGDGMRIGALAVCRDVTVTRPLLH
                     VMNEANQELQALAVSDPVTGLYNHRHFQAALRVEVERARRHNRSTSLLMIDVDLFKPI
                     NDTYGHAFGDEVLRELARAIEGELRATDVPCRYGGDEFAVVLPETDADEARRVAQRML
                     DTVRALPVRPPRGSEALRLSLSIGVATYPEDGETAGDLIASADSRLYLAKQSGRSQVA
                     AETERPGSLAR"
     gene            complement(2161..>2411)
                     /locus_tag="PLQ54_12590"
     CDS             complement(2161..>2411)
                     /locus_tag="PLQ54_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94147.1"
                     /translation="PRNWLFARLRSSDLSGIVKLKATRAPELLTVEEQYQLEHRPWLA
                     RIVRAYRTVYCRVRRPRWRPGYGPSTEDGVAERSETVD"
BASE COUNT          406 a          806 c          790 g          409 t
ORIGIN      
        1 ccacgtgctc ggggtagtag tgccacccga ggaaaggagc ggcccatgcc tctacctgtt
       61 cgccgccgat ggccacatcg ccggggctgg ccactgccga ggacacgagg gtgatggcgc
      121 acccgaccac tgtcaccgag gacctccatg tggcatgtgc catagctggg gcacgttgca
      181 tactgtgatt cgtgcggtcc gttccttgtc gctcgccgat gggctggccg ccacctcggc
      241 ctgacgcgcg agggccacgg gtcggccagc gatcaagccg tggactcgaa cgtgaccctg
      301 gccgccttgc tgttgtaggc ggcctgggcg ttgagcactt tgccgagggt gccgtacgga
      361 gcgaaggtcg accccttgac cgtcacagcg tcgttcttgc ccggtcccac tttgatggac
      421 ttgcacacgc tgcctttaca cagtacgacg cgcttgccct ccgcgttgta ctcgaccttc
      481 cagcccaggg ctttggccac tggtgagatc ggtacataca cggtctggtt cctctcgaag
      541 ggcgcggcgg gcgtcttgat gacctttccg tcgacgacaa ctgtgatcgc cgctgcggag
      601 cctggcccag cgccaacaca caccacgaac gccatcgcga gcacgagcgc tgaacggagc
      661 atttgcacca cctctgtgtg gctcaacgtg cgagggaccc ggggcgttcc gtctcggcgg
      721 ccacctggct ccgcccggac tgcttggcca ggtacagccg ggagtcggcg ctggcgatca
      781 ggtcgccggc ggtctcgccg tcctcggggt acgtggccac gccgatgcta agagagagcc
      841 gcaaggcctc cgatccacgt gggggacgaa caggcaaggc ccggactgtg tcgagcatgc
      901 gttgggccac acgacgagcc tcgtcggcgt cagtctcggg caagaccacg gcgaactcgt
      961 cgcccccata gcggcacggc acgtccgtgg cacgcagctc gccctcgatg gcgcgggcga
     1021 gctctcgcaa cacctcgtcg ccgaacgcgt gaccgtaggt gtcattgatg ggtttgaaga
     1081 ggtcgacgtc gatcatgaga agcgaagtgg agcggttgtg ccgtcgagcc ctttcgacct
     1141 caacccggag agcggcctgg aagtgccggt ggttgtaaag cccggtcact ggatcggaga
     1201 cggccagggc ctggagttcc tgattcgcct cgttcatcac gtgtagcagc ggccgggtga
     1261 ccgtaacgtc gcggcatacc gcaagcgcgc cgattcgcat cccatcgcca tcgcgcagga
     1321 cggaccagtt gatctcgacc gcgatgcgcc gatccgtgca tcggttcacg atgaatgtct
     1381 cgaagcccgt cagggagtcc ctggtgtcca gcgcgcccca gacgaggtcc tcgtactccc
     1441 cgagctcgat cagcgactcg attggccgcc cgaccatctc ggaagcaggg agtccgatga
     1501 gacgcaccgc cgcatcgttg gccaagcgga tgacgcggtc cgttccggcc gcgaccaggg
     1561 cgtcagggca atcccgcacg acgctctccc agagcgcccg gtccgatgcc gcacgcgtgg
     1621 gcgtgtcgcc gacccgttcg agttcgtgct ggcgcacgag ggcttcgaga cggtggtgct
     1681 cctccagacg ccctcgccgg cgatggatgg ccttctcgac ggcttcggcc aggacgccga
     1741 cgccgaaggg cttctccagg tagtcgaagg catcaaagcg cacggcggcg atggccgagt
     1801 ccagagagct ctgccccgtc acgatgatga cctcagtgtt gggtgatatg tgcttgatct
     1861 gacgaagcac atcggccccg ggtatgtccg gcagagcctg gtccaggagg acgaggtcat
     1921 actcggccgc gcgaacgcga tcgatggcgt cgcgacccag gccgactgcg tcggcggtgt
     1981 agcccaggct gaacagcagg tcgaccagaa cctgctgaat gtcgggatcg tcgtcgacga
     2041 ccagaaaacg gtgcgcgtca gccctcatgg cagcgccgcc tccgccgaga gccggcgcgc
     2101 ggcgcgcccc ctgctggcca acaggggcct cccaggctcg aacataccag tcccccagac
     2161 ctagtccact gtctccgatc gttcggccac gccgtcctct gtactgggcc catagcccgg
     2221 acgccaccgg gggcgccgca cccggcagta cacagtacga taggctcgaa cgatccgcgc
     2281 cagccagggc cggtgttcta gctggtactg ttcctcaacg gtcagtagtt cgggtgcacg
     2341 cgtcgccttg agcttaacga tccctgagag atcggaggat cgcagtcgcg cgaacagcca
     2401 gttgcgcggc c
//
LOCUS       DAOSPL010000703         2411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43811_length_2411_cov_2.509762, whole genome shotgun sequence.
ACCESSION   DAOSPL010000703 DAOSPL010000000
VERSION     DAOSPL010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43811_length_2411_cov_2.509762"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..612
                     /locus_tag="PLQ54_12595"
     CDS             190..612
                     /locus_tag="PLQ54_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94148.1"
                     /translation="MNLIGGSEGMYTSFSTESIESARARQMIRENPYSNAAPEKWSDP
                     SRTHYNFEFRGTRLSTALDSVAKTGNIRIITRGPMNGNVTLNIQNARPEVALERIASI
                     ANLRVSQRGSTYVVEQEPVRGESSDSHATRPDDTRPPQ"
     gene            674..1102
                     /locus_tag="PLQ54_12600"
     CDS             674..1102
                     /locus_tag="PLQ54_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HOS94149.1"
                     /translation="MSTPHGFGSNPDPQHGPPPPPPPPPWPPHGDVPPRAGQPWPPPP
                     QYHPRQPEIEQRAIISLVLGICGLVVCGLLSIPAWIVANAALRECDALSIVGPARGYA
                     TAGRVLGIVGTVMIALGVCFGLGVLGLMMVELLSSVPFHH"
     gene            1270..>2411
                     /gene="lptC"
                     /locus_tag="PLQ54_12605"
     CDS             1270..>2411
                     /gene="lptC"
                     /locus_tag="PLQ54_12605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOS94150.1"
                     /translation="MESDRRKLLLSVGGFGVLMAMTVGMALWSYYARPPGSKARKKGA
                     RDVEVRVESASFQVWAHDERDERLIAEVKAKVVQMSKDRSLIQAKEIDPVVLYRDGKV
                     FMEGRAAAAQYDQGTSSKELTLFGGVVLKEKQGGFTMRAEELHHIGRDNRVFSDQPVT
                     FEFPDATMRTPMIALYVEDNRLECIRQVVLTTRRGATLSARSAQLDVASYVLKMAGNV
                     VANVTVGEAQELAGKRSKAPAASAKTPSKTSNPDSALDKMTRFRLKTHHFEYSLKDGT
                     GSCPSPIIIRTADGSARADQAQITQDLVVLQGNVRALMTMGDEPGRTDIATDVIQYRP
                     GVDEFLAPGQVTVTTAASVFQCGSAKARPSEGRIWMTGGVRGAIDR"
BASE COUNT          441 a          805 c          777 g          388 t
ORIGIN      
        1 cggacggcgc ccttaccacc cgcgaacaga gataccggtg acccgctacg gcacccggtg
       61 gctccgaccg aggtcgcccc gtcccccggc agcgtggccc caccgccgac gccctccccc
      121 tcgctctctg cacccgacgc gcagccccgg gatagaagtg actccgcagc cgaacgcagg
      181 cgagcggcca tgaacctcat cggcggctca gaaggcatgt ataccagctt cagtacggag
      241 agtattgaga gtgcccgggc gcgtcagatg atccgggaga acccctacag caacgcagcg
      301 ccagagaagt ggagtgaccc gagccggact cattacaact tcgagtttcg cggcacacgc
      361 ctctcgacag cgctcgactc ggttgcgaag acgggcaaca tccggatcat cacccgcgga
      421 cccatgaacg gcaacgtcac cctgaacatt cagaacgctc gccccgaggt tgcgctcgag
      481 cgcatcgcga gcattgccaa tctgcgcgtc agccaacgcg gctcgacata cgtggtggag
      541 caagagccgg tgcgaggcga gagttcggac tcgcacgcaa ctcgtccgga tgacactcgt
      601 cctccccaat aggccggtgg gttgctctcc gccgacgagg cagaggcgcc ggctgacgaa
      661 ggaggggagc acggtgtcca caccccatgg gtttggctcg aatcccgatc cacaacacgg
      721 accgcctccg ccgccgccgc cgcctccctg gccaccacac ggcgacgtgc cgcccagggc
      781 cgggcaaccg tggccgcccc ctccccagta ccaccctcgg cagccggaga tcgagcaacg
      841 ggccatcatt tccctcgtgc tcggcatctg tggtctggtg gtgtgtggcc tcctgtccat
      901 ccccgcatgg atcgtggcga acgcggccct ccgcgaatgc gatgcgctga gcatagtggg
      961 cccggctcgg ggttacgcga cggccggtcg tgtcctgggg atcgtgggca cggtgatgat
     1021 cgccctgggc gtctgcttcg gcctgggcgt ccttggcctc atgatggtgg aactcctctc
     1081 atcggtgccc ttccaccact agacatcgcc gcagggctca ggcaggtcgc cctctcgccg
     1141 ctgtgaagaa agagcggtgg cggggtgggc accgcgtggc cgctgaaggc cctcgggtcg
     1201 cctgccgtct aatccggtag aggtcgctgc cgcagcctcc agcgggttgg atgacctctc
     1261 aggtgggcta tggagtcgga ccgtaggaaa ctcctgctat cggtcggggg attcggcgtc
     1321 ctgatggcca tgaccgtcgg catggccctg tggtcgtact acgctcgtcc ccctgggtcg
     1381 aaggcgcgga agaagggcgc ccgcgatgtc gaggtgcgag tcgagtccgc ctccttccag
     1441 gtatgggctc acgacgagag ggacgagcgt ctcatcgccg aagtgaaggc caaggtcgtg
     1501 caaatgtcca aggaccgaag cctcatccag gccaaagaga tagacccggt tgtcctctac
     1561 cgcgacggca aggtgttcat ggaaggccga gctgcggctg cccagtacga tcagggcaca
     1621 agcagcaagg aacttaccct ctttggtgga gtggtgctta aggagaagca gggcggcttc
     1681 acgatgcggg ccgaggagct ccaccacatt ggccgcgaca atcgtgtgtt ctccgaccag
     1741 ccggtgacgt tcgagttccc tgacgcaacc atgcgcacgc cgatgatcgc cctctatgtc
     1801 gaggacaacc ggctggagtg tatccgccag gtggtgctga ccacccggcg aggtgcgaca
     1861 ttgtcggccc gatcggctca gcttgacgtg gcctcatacg tcctcaagat ggccggcaat
     1921 gtcgtcgcga acgtcacggt gggcgaggcc caggagctgg ctggcaaacg ctcgaaggcg
     1981 cccgccgcat cggccaagac tccgagcaag acctcgaacc cggatagcgc cctcgacaag
     2041 atgacgcgat tccgcctgaa gacccaccac ttcgagtata gtctcaagga tggcaccggc
     2101 tcctgcccaa gcccgatcat cattcgcacg gcagacggct cggctcgggc cgatcaggcg
     2161 cagatcaccc aggatctcgt ggtactccag gggaacgtcc gcgccctcat gaccatgggc
     2221 gatgagcccg ggcggacgga catcgccaca gatgtcatcc aataccggcc gggcgtagat
     2281 gagttcctgg ccccggggca ggtcactgtc accacagcgg cgtcggtctt ccagtgcggc
     2341 tccgccaagg cgaggccctc ggagggccgc atctggatga ccggtggggt cagaggcgcc
     2401 atcgatcggt g
//
LOCUS       DAOSPL010000704         2410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43829_length_2410_cov_3.061571, whole genome shotgun sequence.
ACCESSION   DAOSPL010000704 DAOSPL010000000
VERSION     DAOSPL010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43829_length_2410_cov_3.061571"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(439..1143)
                     /gene="aqpZ"
                     /locus_tag="PLQ54_12610"
     CDS             complement(439..1143)
                     /gene="aqpZ"
                     /locus_tag="PLQ54_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HOS94151.1"
                     /translation="MPIGKRAAAEFIGTFWLVFGGCGSAVLAANFPEVGIGLVGVSLA
                     FGLTVLTMAYAIGHISGCHLNPAVSLGLWAGKRFRGSDLLPYIAAQLAGAIAAAGVLY
                     VVANGNPEFDLKGGFASNGYGEHSPGFYPLESGLLAEIVLTFMFLFVILGSTDSRAPA
                     GLAPIAIGLCLTLIHLISIPITNTSVNPARSTGPAVFVGGWALQQLWLFWVGPIVGAL
                     ASGLVYPRVAGEAKPE"
     gene            complement(1345..>2410)
                     /locus_tag="PLQ54_12615"
     CDS             complement(1345..>2410)
                     /locus_tag="PLQ54_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94152.1"
                     /translation="EIVGEGGLLEYHATGSPPGGGVYLPQIDTYFDYILRGEGVQAEY
                     TNPDYLRYFVSTYHISNSIGVLCNNNDYPLDQAFVDSLLDRNIRLHYLLGGPRDQRTQ
                     AMRKWYWPALDERLKGRVERAAARRQAAGAGIWEAMRSAREGGEAGLSEVWAANLADP
                     GFVAALGGLGPGEHELPGGWRAYLSPRSDAALSSEGGVLHIDALAHTVACLEHDLPGD
                     VAAVQLRIRATGECGMSWGPAILFRAGNRIFRFGLRSDGKAQGDRPGSQLLFDGYPLG
                     TWFWLRVRLAEGCVVLESSSEGSVWRPVHVEYVGPTDGPRSLAVGKVPYDGSRVEFQE
                     PGGRGQCEFADVRALRGSRP"
BASE COUNT          451 a          880 c          738 g          341 t
ORIGIN      
        1 gaactctatg ccctcgacgg gctcccgctt cgacaagtca ctagatcgca cgaaccgaaa
       61 gctccactgc gcgggtctgc tcgactcata ggccatcagg aaacccttac cgtcccggca
      121 gaccgatgtg ttgagcaggt gctcgtcccc tcgacgcgtc acgacctgct cctgtctcca
      181 tgtcctcagg tcggtcgacc agaagcagcg gatgtccttg gtccactccg tgttggtgtt
      241 ctcggtagcg aacaggctga gcccatcgcc gttgacgaac ccgctaacga aggagaaccc
      301 cgtcagacga ccgcggtgtt ctccatccct tcgccgaagg cgaacggcag cttggtgagc
      361 accgggttgt ccggcgctgc ctcgcccggg gcgaggaatg ggcagacgct cagggcagac
      421 acgaggcgcc gagggagcct actccggctt cgcctctccg gccacccgcg ggtagaccag
      481 ccccgaggcg agcgccccaa cgatgggccc gacccagaac aaccagagct gctgcagagc
      541 ccaacctcca acgaagaccg ccggccccgt gctccgagct gggttcaccg aggtgttcgt
      601 gatcgggatg ctgatcaggt ggatgagagt gagacacagg ccaatggcga tgggtgccag
      661 gccggcggga gcccgcgagt ctgtcgaccc caggatcacg aacaggaaca taaacgtcag
      721 cacgatctcc gcaagcaggc ccgattccag cgggtagaaa cccggcgaat gctccccgta
      781 gccgttggat gcaaagccgc ctttgaggtc gaactccggg ttgccgttgg cgacaacata
      841 cagcacgccc gcagccgcga tggccccggc caactgcgcg gcaatgtacg gcagcaggtc
      901 agagccgcgg aaccgcttgc cggcccaaag ccccagcgag acggcggggt tcaagtggca
      961 ccccgagatg tgcccgatcg cgtaggccat cgtcaggacc gtgagaccga acgcgaggga
     1021 aacccccaca aggccaatcc ccacctcggg gaaattggcc gccagcaccg cactgccgca
     1081 accgccgaac acgagccaga acgtcccgat gaactccgct gccgcacgct taccgatggg
     1141 caccaaacac cctctcctct ccccaagccg cgccgacgat gcggctcgtt gcagaccaac
     1201 ctaggaacga ggaacccagt gaactccaac ggtgggcccg gtgaccgggc tgcgcgacct
     1261 cgagcatcgc ggcatagtta ccatgttcac gtaagcacca cgccactcct ttgccacgac
     1321 gcgcggctga gcgccgacag gggcctaggg gcgggagccg cgcagggcgc gcacgtcggc
     1381 gaactcgcac tggccacgcc cgcccggctc ctggaactcg acgcgcgagc cgtcgtacgg
     1441 gaccttaccc accgccagcg accgcggccc gtccgtcggc ccgacgtact ccacatgaac
     1501 cggccgccac accgacccct cggacgatga ctccagcacc acgcacccct cggccagccg
     1561 cacgcgaagc cagaaccacg tacccagcgg gtagccgtcg aacagaagct gagaccccgg
     1621 acggtcgcct tgagccttcc catctgaccg caggccaaac cggaagatcc ggttcccggc
     1681 ccggaacaag atcgccgggc cccatgacat gccgcactcg cctgtcgcgc gaatcctcag
     1741 ctgcacggcc gcgacgtccc cgggcaggtc atgctccagg caggccaccg tgtgcgccaa
     1801 tgcgtcgata tgcagcacgc ccccctcaga ggagagcgcc gcatcgcttc tcgggctgag
     1861 gtaggctcgc cagccgccgg ggagctcatg ctcgccgggc ccgagtcccc caagggccgc
     1921 aacgaagccg gggtccgcca ggttggccgc ccacacctcc gacagcccgg cttcgccacc
     1981 ctcccgggcc gatcgcatcg cctcccagat cccggcccca gcggcctgtc gccgagccgc
     2041 ggcgcgctcc acgcgaccct tcaggcgctc atcgagcgcc ggccagtacc acttccgcat
     2101 ggcctgcgtc cgctggtctc tggggccgcc cagcaggtag tgcagtcgga tgttccggtc
     2161 cagcaggctg tcgacgaacg cctggtccag cgggtagtcg ttgttgttgc acaacacccc
     2221 gatggagttg ctgatgtggt acgtcgagac gaagtagcgc aggtaatcgg ggttggtata
     2281 ctcggcctgg acgccctcgc cgcgcaggat gtagtcgaag taggtgtcga tctgcggcag
     2341 atagaccccc ccgcccggtg ggctccctgt ggcgtggtac tcgagcagcc ccccttcgcc
     2401 gacgatctcc
//
LOCUS       DAOSPL010000705         2410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43840_length_2410_cov_2.423355, whole genome shotgun sequence.
ACCESSION   DAOSPL010000705 DAOSPL010000000
VERSION     DAOSPL010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43840_length_2410_cov_2.423355"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(335..1630)
                     /locus_tag="PLQ54_12620"
     CDS             complement(335..1630)
                     /locus_tag="PLQ54_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOS94153.1"
                     /translation="MSRFDGQRLPPATFGLDVDGLRSGVYSDRYFVNMQRIFERLAQK
                     GAAAGDIEVEMQWFCRRKPAALVAGVDEALAMLRTCAGTMDPSGQLISSYDQLEVLAI
                     HDGDIVSYHGDPSRVEPVLVVRGRYRDFVTLETPTLGALSRGSRIATNVYRVLEAAGG
                     KPVLFFPARFDSHRTQSADGYAYWVAVQRYNHDHGARVRPFVSTDAQGAWWGGAGSGT
                     VAHGYIGAFHGDTAAAMVAFAEECPLEVPRIALVDYHNDCVGDSLRVARALFERWAPC
                     AEAGDAAGMARYELYAVRLDTSGDMLDLSLAANADPSARGVSPALVRAVRRGLDNAWS
                     EWGLTGARRDAARDFCRAVRIVATGGFSVERIRAFESDGVPVDIYGVGSAVFDNSGAT
                     GTNTDFTADVVRVRQGGQWIDMAKAGRRACPNPLLQPVA"
     gene            complement(1627..>2410)
                     /gene="ispF"
                     /locus_tag="PLQ54_12625"
     CDS             complement(1627..>2410)
                     /gene="ispF"
                     /locus_tag="PLQ54_12625"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOS94154.1"
                     /translation="VPIIDTVKLSEAGERVERTLPRHALWAVQTPQTFQTGLIRRAHA
                     EAQRLGAALTDDASAVELLGEPVYLFPGDPENLKITTPLDFEIAEAIARRRDGAEVPP
                     LRTGIGYDAHRIDPTRPLILGGVRIDSPFGLLGHSDADVLTHAIMDALLGAVGAGDIG
                     AHFPDSDPAYRGACSLDLLRRVTGLVAERGYMVSSIDAVVVAQAPRLAPFVGAMRVAL
                     AEALGVGEDCVSVKATTTEGMGFEGRGEGISAQAVANVRRCT"
BASE COUNT          380 a          825 c          851 g          354 t
ORIGIN      
        1 ctccgtcctc ggcgcgaccc ggacattggc ccgtcggtcc tgcagccgat ggagctgctc
       61 atcgtcctca cgccccgggt gacggcggca gaggcaccgg cggcccagga gcccgcacca
      121 gccacccctg cggcgacgcc tcggaagtag tcgcgggagg ctcacggcgc gcgccatcct
      181 ggcctgctac ggcctctccg tactagcctt cggggctcac cgctgggcgc cgcggagcgt
      241 gacggccgcg gtaacgcagg cggaagccac gtcggggtcc agggggacca gtccccctgg
      301 cagggatcgg gacggagtcc cgaggttctc ggcgctaggc caccggttgc aggagcgggt
      361 tcgggcatgc gcggcggccg gccttggcca tgtcgatcca ttggccgccc tggcggacgc
      421 gcaccacgtc agcggtgaag tcggtgttgg tacccgtggc gccgctgttg tcgaacacgg
      481 ccgagccaac accgtagatg tcgaccggca cgccgtcgga ctcgaaggcg cggatgcgct
      541 cgacgctgaa gccgcccgtc gccacgatcc gtacggctcg gcagaagtcc cgtgccgcgt
      601 cgcggcgggc gccagtcagg ccccattcgg accaggcgtt gtccagaccc cgccgcaccg
      661 cgcgcaccag cgccggactc actccccggg ccgatgggtc ggcgttcgcg gcgaggctga
      721 ggtcgagcat gtcaccactc gtgtcgagcc gaaccgcgta gagctcgtat cgcgccatac
      781 cggccgcgtc gccggcctcc gcgcacggtg cccaccgttc gaagagggca cgagccaccc
      841 gcagactatc gcccacacag tcgttgtggt agtcgaccag cgcgatgcgg gggacctcca
      901 atgggcactc ctcggcgaag gccaccatgg ccgcggccgt atccccgtga aatgctccaa
      961 tgtacccgtg agccaccgtt ccactgcccg ccccgcccca ccaggcgccc tgggcgtcgg
     1021 ttgatacgaa gggccgcact cgggccccgt ggtcgtggtt gtagcgctgc acggcgaccc
     1081 aataggcgta gccgtcagcg gactgggtcc ggtgggagtc gaaccgggcc gggaagaaga
     1141 gcaccggctt gccgccggcg gcctcgagca cccggtacac gttggtggcg atccgcgagc
     1201 ctcgcgacag ggcaccgagg gtgggtgtct cgagggtcac gaagtcgcgg tagcggcccc
     1261 gcaccacgag aacgggctcg acccgcgaag ggtcgccatg gtaggagacg atatcgccgt
     1321 cgtgaatggc aaggacctcc aactggtcat atgagctgat cagctgacca gatgggtcca
     1381 tggttccggc gcacgtccga agcatggcga gggcctcgtc gacaccggcg acgagtgccg
     1441 caggcttgcg gcggcagaac cactgcattt cgacttcaat atcaccggct gcagcgcctt
     1501 tctgggccag ccgctcgaag atccgctgca tgttgacgaa atagcggtcc gaatagacgc
     1561 cgctgcggag gccgtccacg tcgagtccga acgtagcggg gggcagcctc tggccgtcaa
     1621 atcgactcat gtgcaccgcc tcacgttcgc cacggcctgg gccgagatcc cctcgccacg
     1681 gccttcgaag cccatgccct cggtggttgt tgctttgacc gacacgcagt cctcgccgac
     1741 tccgagggcc tcggccaggg cgacccgcat ggcgcccacg aagggcgcca gtctcggtgc
     1801 ctgggcgacg accacggcat cgatgctcga gaccatgtag ccgcgctcgg cgaccagacc
     1861 ggtgacgcgg cgcagcaggt ccaggctgca ggcgcctctg taggcggggt ctgagtccgg
     1921 gaagtgggcg ccgatatcgc ccgcgcccac ggcacccagc agggcgtcca tgatggcgtg
     1981 ggtgagcacg tcggcatcgg agtggccgag cagaccaaag gggctgtcga tcctcactcc
     2041 gccgaggatc agcggacgtg tcgggtcgat acgatgggcg tcatagccga tgccggtccg
     2101 caaggggggc acctccgcgc catcgcgccg cctcgcgatg gcttcggcaa tctcgaagtc
     2161 aagcggcgtg gtgatcttga ggttctcggg gtcaccaggg aacaggtaga ccggttcgcc
     2221 gaggagctcg acggccgaag cgtcgtcggt cagggcggcg ccgagccgct gggcctcggc
     2281 gtgggcccgg cggatcagac cggtttggaa ggtctggggt gtctggaccg cccacagggc
     2341 atggcggggg agcgtgcgct ccacgcgctc gccggcctcg gagagcttca ccgtgtcgat
     2401 gatgggcact
//
LOCUS       DAOSPL010000706         2407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43905_length_2407_cov_3.625850, whole genome shotgun sequence.
ACCESSION   DAOSPL010000706 DAOSPL010000000
VERSION     DAOSPL010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43905_length_2407_cov_3.625850"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..555)
                     /locus_tag="PLQ54_12630"
     CDS             complement(238..555)
                     /locus_tag="PLQ54_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94155.1"
                     /translation="MRRAIAMLCGLALLAAMALTGLGCKGSGPGNPKPAPEDQNAKMM
                     QMQKQMMQSKMGAKGPGPASGVGTETSGKAADPADGTAACPAEGATESAADSAGATEK
                     KTE"
     gene            complement(609..1349)
                     /locus_tag="PLQ54_12635"
     CDS             complement(609..1349)
                     /locus_tag="PLQ54_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOS94156.1"
                     /translation="MTRRGFTLIELLVVIAIIAILAAILFPVFAKAREKARQTSCLSN
                     CKQIANALIMYTTDYDETMPFITSCSAPEYTHHAAANAQGKLHPYIKNAQVWQCPSAK
                     RGMQLTANPGRNGGVAESGSWTFPLEFAGILLSYGGTERVMRNLDCNFGGTPLRIAEM
                     QAPASVTAFTESPILSNCGCMRAIWPDACCAARDNPGERLDKNTRHNGGNNLVFCDGH
                     AKWMKAEAMVAKWQQPYVQGAYNELFWP"
     gene            1733..2200
                     /locus_tag="PLQ54_12640"
     CDS             1733..2200
                     /locus_tag="PLQ54_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94157.1"
                     /translation="MRAHDRVVIVVAVAGALITCSSCKRQPVGAAPAGKSPPQAKGFS
                     MPEMPAAPKVADEEVRKLGIPVFAGAVGMGTRKIADDTNISLLVEKGIAEVDAWYAKE
                     LKDWKRVEDAGETFKSVVYTSPDTLSIVSLMASPSVPNRTSIRLVRREPPRPK"
BASE COUNT          379 a          777 c          822 g          429 t
ORIGIN      
        1 ggccgccgcg ctgcacgcgc tggaggtgct ggcgccgtgc gggggattcc ggctgggcga
       61 cggcgccaat gtgtgtccgg gcacgccgct ggagaacctg gcggcactga ccgaggcggc
      121 ggaggagtac ggcgtgccgc atcccgggcg cccgctccag tagcgagaag ggacccggct
      181 cagcgagccg ggccccgtct ccgcgccatg gtgggccgcg ccgcccgggc ccgaccccta
      241 ttcggtcttt ttctcggtcg ccccggcgga atcggccgcc gactcggtcg cgccctcggc
      301 cggacaggcc gctgtgccgt cggcgggatc agccgccttg cccgaggtct ccgtcccgac
      361 gccggaggcc ggaccgggcc ccttggcacc catcttgctc tgcatcatct gcttctgcat
      421 ctgcatcatc ttggcgttct ggtcttcggg tgccggtttg ggattgcccg gtccggagcc
      481 cttgcagccc agtccggtta gtgccatcgc ggccagcagg gccaggccgc acagcattgc
      541 gatcgcgcgc ctcacactca caccgccctt cacagcattg ctcgtcagga gtggcccagc
      601 tgacccgcct agggccagaa cagctcgttg tacgcgccct gcacataggg ctgctgccat
      661 ttggccacca tggcctccgc cttcatccac ttggcgtggc cgtcgcagaa gaccaggttg
      721 ttgccgccgt tgtggcgggt gttcttgtcg aggcgctcgc ccgggttgtc tcgggcggcg
      781 cagcaggcgt cgggccagat cgctctcatg caaccgcagt tggagaggat gggcgactcg
      841 gtgaatgccg tcacactggc gggagcctgc atttcggcga tgcgcagcgg ggtgcccccg
      901 aagttgcagt ccaggttgcg catgacccgc tcggtgccac cgtaggatag caggatgcct
      961 gcgaactcga gagggaatgt ccaggagcca ctctcggcca ccccgccatt acggccgggg
     1021 ttggccgtca actgcatccc ccgcttggcg gaagggcact gccacacctg ggcgttcttg
     1081 atataggggt ggagcttgcc ttgggcgttt gcggcggcat ggtgcgtgta ttcgggggca
     1141 ctgcaggagg tgatgaacgg catggtctcg tcgtagtcgg tcgtgtacat gatgagcgcg
     1201 ttggcgatct gcttgcagtt ggacaggcac gatgtctgcc tggccttctc ccgagccttc
     1261 gcgaagaccg ggaagaggat cgccgcgaga atcgctatga tcgcgatgac gaccagtagt
     1321 tcgatcagcg tgaacccgcg tcgtgtcatc tgcgctgcac cctccactcc gtcgtggttt
     1381 cccgggcacg ctcagcgcac ccgctcggac acactttggc acgttagcca gcacccggct
     1441 cacacccgga gccgtcacga tgcgacctcg ggaaccacct cctctggttg cgtacctagc
     1501 acatccggta acccgagtca attgctgttc ttatcgagcg gacggacgcg actcgccagc
     1561 ggacggggtg acctgacgag gccgaggcac cggtccacgc atgggccgag aaggaccggc
     1621 gggcatgcgt ccgaagtgga gagtgttcat cgaacggcac ccaaccgagt cgacgaccgg
     1681 gccagcggtt cgaggcctgc gtgccgacgt ctttcccggg ggaggaacgg cgatgcgtgc
     1741 ccatgatcga gtggtgatcg tcgtggcggt ggccggggct ctgatcacgt gttcttcctg
     1801 caagaggcag cccgtgggtg ccgctcccgc gggcaagtcg ccgccccagg cgaaaggctt
     1861 ctccatgccc gagatgccgg ctgcgcccaa ggtggcggac gaggaggtca ggaagctagg
     1921 cattcccgtt tttgcgggag cggttggcat ggggacgagg aagattgccg acgacacgaa
     1981 catcagcctc ctcgtcgaga agggcatcgc ggaggttgac gcctggtatg ccaaggagct
     2041 caaggactgg aagagggtcg aggatgcggg cgagaccttc aaaagtgtgg tgtacaccag
     2101 ccccgatacc ctgagcattg tctcgctgat ggcctcgccc tctgtgccga accgaacgtc
     2161 catccgcctg gtgcggcggg agccgccccg acccaagtag aagcgagacc gccgaacccg
     2221 agacagggct cggcggtctc cacatggtgc gcagggatat cagggctcag ttggcggggt
     2281 taccccagcg ggcccaatag gtccgtgtgt ccgtgccact cgggtagacg atacgtccgc
     2341 agttgtcgcc cagccagcgc gagttgtgcc acttggcatg gccatcggcg tagcacacgt
     2401 tctcgcc
//
LOCUS       DAOSPL010000707         2404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43994_length_2404_cov_3.626650, whole genome shotgun sequence.
ACCESSION   DAOSPL010000707 DAOSPL010000000
VERSION     DAOSPL010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43994_length_2404_cov_3.626650"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="PLQ54_12645"
     CDS             complement(<1..1146)
                     /locus_tag="PLQ54_12645"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOS94158.1"
                     /translation="MTWPLAFEYPAALWLLWLLPVAWLASARSLAGMSTARRRVATVV
                     RSVVLILLVLAAAGVQRVKLRDSMCVLYLVDTSDSMDPTRLATVRSYINEASKHARRD
                     DVSGIVVFGEEALLDTLPVLDLKVRQFLSVPSTNHTDIAQAIRLALAAFPSGSAKKIV
                     LFSDGNENLGNAIEEALIAESNDVVIDVVPLVRNLENEVILHKLTTPPRAKRGEPFEC
                     EVVAEATKETPVIIELWRNDSFIDSYRVNLSGGRKERVSLPALSLASPGFYTFEARLQ
                     VGEGDDTIPENNVGMSFTRVQGEPKALLVSSDVGRARFLHRALTNAHIETRLTTPAAI
                     PLTLGELAAYDTVILDSVNAVNFTAKQMKMVQGAVRELGIGLVMIGGD"
     gene            complement(1186..1503)
                     /locus_tag="PLQ54_12650"
     CDS             complement(1186..1503)
                     /locus_tag="PLQ54_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94159.1"
                     /translation="MRGDDGFRHIREVARPTPMDAVLLIICAGCFTLLLVATRYGWES
                     SGYAFCGGLAFSLWVLNIVNRVIGTLRELHDRVRRLELRLQGLLDDMGDEEAAHGRDE
                     RRN"
     gene            1728..>2404
                     /locus_tag="PLQ54_12655"
     CDS             1728..>2404
                     /locus_tag="PLQ54_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94160.1"
                     /translation="MVRAPRALNLAHVAVLLAHGAALAGALTPNEPLRAPNGDPVCAI
                     YFFPHWWEPWKTSDETVLRDLARLKAMGINTLLVDHEWSQAIDGNWGLLDRGHRLARQ
                     AGLQILPWLSLKTWSDMGCDPGRIGLAREWFGANLTQGVTQDGKPSSLLVYDEETVAA
                     GAAYAEQYVRRYLDEGALARFRWRGTTRPCVALTVELGWDGASFDGRTNGLFRDWLRR
                     RYVDEVSR"
BASE COUNT          418 a          798 c          793 g          395 t
ORIGIN      
        1 gtcgccgccg atcatgacca gaccgatgcc cagctcgcgg acggctccct gcaccatctt
       61 catctgtttg gccgtgaagt tcaccgcgtt gacgctatcg aggatgacgg tgtcgtacgc
      121 cgccagctcg cccagggtga gtgggatcgc cgcgggtgtg gtgagccggg tctcgatgtg
      181 cgcgttggtc agagcgcggt gcaggaatcg agcacggccc acatcggagc tcacgagcag
      241 ggccttcggc tcgccctgga cccgcgtgaa actcatgccg acgttgtttt cggggatggt
      301 gtcatcgccc tcgcccacct gaagacgcgc ttcgaatgta tagaaccccg ggctcgcaag
      361 ggaaagggcc ggcaacgaga cgcgctcctt cctcccgccg ctcaggttca cgcggtagct
      421 gtcgatgaat gagtcgttgc gccacaactc gatgatgacg ggcgtctcct tggttgcctc
      481 ggcgaccacc tcgcactcga atggctcgcc gcgcttcgcg cgcgggggag ttgtcagctt
      541 gtggaggatg acctcgttct ccaagttgcg caccaggggg acgacatcaa tgaccacatc
      601 attggactcg gcaatcagcg cctcctcgat ggcgttcccc aggttctcat tgccgtcgct
      661 gaacaggaca atcttcttgg cagaccctga tgggaatgcc gccagcgcca ggcggatcgc
      721 ctgggcgatg tcggtgtggt tggtcgacgg aacagaaagg aattgcctta ccttcagatc
      781 cagcaccgga agtgtgtcga gaagagcctc ctcgccgaag accacgatgc ccgatacgtc
      841 gtcgcgccgg gcatgcttgc tggcttcgtt gatgtacgaa cggacggtgg cgagccgtgt
      901 gggatccatg ctgtccgagg tgtcgaccaa gtagaggacg cacatcgagt cgcgaagctt
      961 gactcgctgg accccggccg cggcgagcac gagaaggatg agaaccaccg agcgcaccac
     1021 ggtggcgacg cgccggcgcg ctgtagacat gcccgcaaga ctgcgcgcgc tagcgagcca
     1081 cgccacgggc agcagccaga gcagccacag agccgccggg tactcgaagg ccaaaggcca
     1141 ggtcatgggg cacccaggcc aggcttgcgg cccgccgtgg ccaggtcagt tacgtcgttc
     1201 gtcgcgccca tgagccgctt cctcatcgcc catgtcgtcc agcagcccct ggagccggag
     1261 ctcgagccgc cgcacccgat cgtggagctc ccgcagcgtc ccaatcactc ggttcacgat
     1321 gttcagcacc cacagcgaaa acgccaaacc tccacagaac gcgtaaccgg aggactccca
     1381 accgtatcgt gtcgccacca gcaggagagt gaagcacccc gcacagatga tgagcagtac
     1441 ggcgtccatg ggagtgggcc gagccacctc ccggatgtgc cggaatccat cgtctccccg
     1501 caccgtcact gcctccccgc tggaaccggc cggggttcgc tccccgcatc cctcggtcac
     1561 gggtgctcga cgcatggcct gggaccgatg ggcgcccggc cttcagactg gccgcggcct
     1621 ggtggtgccc gtattgtaga cgaacactcc gctgggaggc acggcgagac gacggaggga
     1681 aacgatcaaa gcggggcaac cgcccccgga tgaggagctc acgcgtcatg gtacgggccc
     1741 cccgcgctct caacctggct cacgtcgctg tgcttctggc ccacggggct gccctcgcgg
     1801 gagcactcac ccccaacgag ccgcttcgcg cccccaacgg cgaccccgtg tgcgccatct
     1861 acttcttccc ccactggtgg gaaccgtgga agacctcgga tgagacggtg ctgcgcgacc
     1921 tggcgcggct gaaggccatg ggcatcaaca cgctgctggt cgatcacgag tggtcccagg
     1981 ccatcgatgg caactggggg ttgctggacc gcgggcatcg tctggcgcgg caggcgggac
     2041 tgcagatcct gccctggctc tcgctcaaga cgtggagcga tatggggtgt gaccccgggc
     2101 gcatcggcct ggcacgcgaa tggttcgggg cgaacctcac gcagggcgtc acgcaggacg
     2161 gcaagccctc gagcctgctg gtctacgacg aggagaccgt cgcggctggg gccgcctatg
     2221 ccgagcaata tgtccggcgc tacctcgacg agggcgccct ggcgcggttc cgctggcggg
     2281 gcacgacgcg tccgtgcgtg gcgctcaccg tcgagctagg ttgggatggc gccagcttcg
     2341 acgggcgcac caatggactc ttccgcgact ggctgcgccg gcggtacgtg gacgaggtgt
     2401 ctcg
//
LOCUS       DAOSPL010000708         2404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_43995_length_2404_cov_3.485739, whole genome shotgun sequence.
ACCESSION   DAOSPL010000708 DAOSPL010000000
VERSION     DAOSPL010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43995_length_2404_cov_3.485739"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PLQ54_12660"
     CDS             <1..472
                     /locus_tag="PLQ54_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94161.1"
                     /translation="KESYLEAGKPDAYVEDDIYFTTDSQFAYVASVAGTMARERPAAC
                     FYMGYFYAEALILAEAGAMAGCIQIAGTDAVMQLPFLVTTCDYTLMGEELYAATAYLT
                     REPRLLGSLKAQDWLKMAVAAALVCGVVFAGLGQILHLPETSWMLKFISLFETR"
     gene            493..1194
                     /locus_tag="PLQ54_12665"
     CDS             493..1194
                     /locus_tag="PLQ54_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94162.1"
                     /translation="MRTQVPVAIAFIAGVVMVIQFFVPTHHSMALRDTLLAWTRIISA
                     FALVVGIGSLVSINISKLRRLSPGWVYAIVTLAGFAVMAVFGILGTKEAAAPAWRHTL
                     DRCYQWLFDNVQVPMDATIFSLLAFFIASAAYRTFRARTFEASLLLAAALLVMVGRVP
                     LGVEIGAKLHEAIWGGSTTAPVTPEWLQSLTEWVLNCPNTAAQRAIMFGAALAAVAQA
                     LRIIVGIERPYMSGS"
     gene            1200..2057
                     /locus_tag="PLQ54_12670"
     CDS             1200..2057
                     /locus_tag="PLQ54_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94163.1"
                     /translation="MSHTPDAASGNRDFWVRLQRIDRRWIFLVLFVGVVVPYFTPLGC
                     RVRPSDPTRKLYDFIDKLPERKPVMISFDYGPGSAAELDPMAVAIARHCFKKNLRLIG
                     VTLDPTGALLIRKAFEKAGKDLGKKMHEDWVSLGYKPGGGVVVLGIGGGLDTVFATDS
                     EGKPYGEIPALEGVRNYDDIGIVVDLASSAAPDIWIRYAREAFKANLACGVTGVMVAA
                     YYPYLQTGQFIGILGGLKGAAEYEQLTEAPGDGQIGMDSQSVAHLIVIALVILGNVAS
                     IASRRGPRG"
     gene            2070..>2404
                     /locus_tag="PLQ54_12675"
     CDS             2070..>2404
                     /locus_tag="PLQ54_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94164.1"
                     /translation="MEHPGFWHVFSVWVAALLTLIAYSFLYKDNPAYRFVEALFVGVS
                     AGYGLAIVYRDVVIPDLWKPIHDEGDLLRLIPAVIGVLMLSRFHPKISWLSRWPMAFT
                     IGIGAGMSIP"
BASE COUNT          378 a          749 c          791 g          486 t
ORIGIN      
        1 caaggagtcc tatctcgagg ccggcaagcc cgatgcatat gtcgaggatg acatctactt
       61 cacgacggac tcccagttcg cctatgtggc ctcggtggcc ggcaccatgg cccgcgagcg
      121 gccggcggcg tgcttctata tggggtactt ctacgccgag gcactgatcc tggccgaggc
      181 tggcgccatg gccgggtgca ttcagatcgc gggcacggac gccgtcatgc agttgccgtt
      241 tctggtcacg acatgcgact acacactgat gggcgaggag ctctatgccg ccacggccta
      301 tctgacccga gagccgcgcc tgctgggtag cctcaaggcg caggattggc tgaagatggc
      361 ggtggcggct gccctcgtgt gcggcgtggt cttcgcgggc ctggggcaga tcctgcatct
      421 gcccgagacc agttggatgc tcaagttcat atcgctgttc gagactaggt aaacggaggc
      481 accgcgaagc ccatgcggac tcaagtgccg gttgccatcg cgttcatcgc cggtgtcgtc
      541 atggtgatcc agttctttgt gccgacccat cactcgatgg ccctgcggga cacgcttctg
      601 gcgtggaccc gcatcatcag cgcctttgca ttggtcgtgg ggatcggcag cctggtctcg
      661 attaacatca gcaagctgcg gcggctttcg cccggatggg tctacgccat cgttactctg
      721 gcggggttcg ccgtgatggc cgtgtttggg attctgggga caaaggaggc cgccgcccct
      781 gcgtggaggc atacgctcga ccggtgctac cagtggctgt tcgacaacgt gcaggttccc
      841 atggacgcga ccatcttctc tttgctggcg tttttcatcg catcggccgc ctatcggacg
      901 ttccgggcga gaaccttcga ggccagcctc ctgcttgccg ccgcgctgtt ggtgatggtc
      961 ggccgggtcc cgttgggtgt cgagatcggc gccaagctcc atgaggccat ctggggcggg
     1021 tcgacgacgg cgccggtgac acccgagtgg ctccagtcgc taacggaatg ggtgctcaac
     1081 tgcccgaaca cggccgccca gcgcgccatc atgttcggtg ccgccctggc ggccgtagcc
     1141 caggcgctgc gaatcatcgt ggggatcgag cgcccctaca tgagtgggag ctgatccgga
     1201 tgtctcacac tccggacgcc gcgtcgggaa accgcgactt ctgggttcgg ctccagcgta
     1261 tcgaccggcg ctggatcttc ctggtgctgt tcgtcggggt tgtggtcccc tacttcaccc
     1321 cgttgggttg ccgggttcgg ccgtccgatc ccaccaggaa gctctatgac ttcattgaca
     1381 agctccccga gcggaagccc gtcatgatct cgttcgacta cggtccgggc tccgcagcgg
     1441 agctggaccc gatggcggtg gccattgccc ggcactgctt caagaagaac ctccggctca
     1501 tcggtgttac gctcgatccc acgggtgccc tgctcatccg gaaggcgttc gagaaagcgg
     1561 gcaaggatct gggcaagaag atgcatgagg actgggtcag cctgggctac aagcccgggg
     1621 gcggcgtcgt ggtcctcggc atcggaggcg gcctcgacac ggtgttcgcg acggacagcg
     1681 agggcaagcc gtacggcgag atcccagcgc tcgagggagt ccgaaactac gacgacatcg
     1741 gcattgtggt ggatctggcc tcgtcagcgg cgccggacat ctggatccgc tacgcacgcg
     1801 aggcgttcaa ggccaacttg gcttgtggcg ttaccggtgt tatggtcgcc gcctactacc
     1861 cttatctgca gacggggcag ttcatcggca ttctcggcgg gctcaagggg gcggccgagt
     1921 atgagcagct caccgaggca ccgggcgatg gccagatcgg tatggactcg cagtcggtgg
     1981 ctcacttgat cgtcatcgcg ctggtcatcc tgggcaacgt ggcgtccatc gcgtcccgtc
     2041 gcggcccgag aggataaggg cagatcggca tggagcaccc ggggttctgg cacgtcttca
     2101 gcgtctgggt cgccgcgcta ctgaccctga ttgcatacag cttcctttac aaggacaacc
     2161 cggcctaccg cttcgtcgag gccctgttcg tgggggtctc cgccggctac ggcctggcga
     2221 tcgtctaccg cgatgtggtc atacccgacc tgtggaaacc gattcacgat gagggcgact
     2281 tgctgcggct catcccggcc gtcatcggcg tgctcatgct gtcccgattt cacccgaaga
     2341 tctcgtggct ctctcggtgg cccatggcgt tcacgatcgg catcggcgcc ggcatgtcga
     2401 tccc
//
LOCUS       DAOSPL010000709         2403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44028_length_2403_cov_3.067291, whole genome shotgun sequence.
ACCESSION   DAOSPL010000709 DAOSPL010000000
VERSION     DAOSPL010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44028_length_2403_cov_3.067291"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..1250
                     /locus_tag="PLQ54_12680"
     CDS             216..1250
                     /locus_tag="PLQ54_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94165.1"
                     /translation="MRFIQARFGHAARVAFMVVGLLGFAGPSHGYVGDYESTYWRAWI
                     MVSQGKWSDAAKSARISLAHMAKRYGIVEAQMARFEERAATASPEDRAALRQELGFLA
                     KRLAWMDVRKNGNEWIVAEAEKPGAGVLPYIQEDAALVVSLYQQATLYQVRAANYEQR
                     RGRFDEADAYHQEALRRWDDAAKQMGDRLAALRAQVSGREPSAAEAARLAFLEEWAGR
                     YADTATGLRSEYERLRDPRALVANGLFYRQRARLYEDDPDSEFRSTYRARVQFYFDKA
                     LWRCRQLLDRRDADPSLKALESSLDKEQPDPEGEPCPASYYDAVTEEYKMYTYFYEFW
                     REGATGISID"
     gene            1356..>2403
                     /locus_tag="PLQ54_12685"
     CDS             1356..>2403
                     /locus_tag="PLQ54_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94166.1"
                     /translation="MRPGARPGPVGASGPPKLRSSFWDSPAGLLLYNGAQQAARVALV
                     AVGLSLVFLGWGVLGGHLVNAQFADSPDTAQFRLWSTVFCYGCLLLAVSVVIRYHQED
                     GYMMSLLGLGAFATFGVPFLLNSMLGALNADLSSHAGADAVNDAGRASGIAIFILVGA
                     RFLVYGALRLMAAAAHKVEPAPEGFEVLVEKSGGQAARPAPGSPRTFAACWELPFCSE
                     YVRSTCPRFAERKSCWKRKSGCHCDVDLLGAALGLDAVEKGLRSANRITRQALQDASG
                     RKIACGECRIFIEHQRRKFRLLSAFALPVTILLLLALRPLIGQLWGAAVVGLARVTQV
                     VAFSSPGTERATQLV"
BASE COUNT          365 a          726 c          896 g          416 t
ORIGIN      
        1 tgatcccgct cgagagctcg ctgacgaccg gggcgggccc ggcggagacg aggaggacag
       61 ctaggaccca atagaccgaa cccaccgcgg ttcctcacgg gttgattgcg aggatcagaa
      121 cccacgcgat gtcttcactt gcggcccgcg actccctgct tcgcagaacc agaaggacgg
      181 cagggcgcgc ggactcacga ccgagaggtg cgcccatgcg cttcattcag gcacggttcg
      241 gccatgcagc acgcgttgcc ttcatggtgg ttggactgct gggattcgcg ggaccttcgc
      301 acggctatgt gggcgactat gagtcgacgt actggcgggc gtggatcatg gtgagccagg
      361 gcaagtggag cgatgcggcc aagtccgccc ggatctcgct cgcccacatg gccaagcggt
      421 atggcatcgt ggaggcccag atggcccggt tcgaggagcg cgcggcgacg gccagccccg
      481 aggaccgggc cgcgctcagg caggaactgg ggttcctggc caagcgcctg gcgtggatgg
      541 acgtccgaaa aaacggaaac gaatggatcg tggccgaagc tgagaagccc ggcgcgggcg
      601 tgctcccgta catccaggag gacgcggcgc tggtggtgtc gctgtaccag caggccacgc
      661 tgtaccaggt tcgggcggcc aactacgagc agcggcgcgg acggttcgac gaggcggatg
      721 cgtaccatca ggaggccctg cggcgttggg acgacgctgc caagcagatg ggcgatcgcc
      781 tggcggccct gcgggctcag gtgtcggggc gggagccgtc agccgccgag gcggcacggc
      841 tcgcgttcct tgaggagtgg gccggtcggt acgcggacac ggccacggga ctccgttcgg
      901 agtatgagcg gctgcgcgac ccgcgggcgc tggtggcgaa tggtctgttc taccgccagc
      961 gcgcgcggct ctatgaggat gacccggaca gcgagttccg ctcgacctac cgagcgcgcg
     1021 ttcagttcta cttcgacaag gccctgtggc ggtgccggca gctgctcgat cggcgggatg
     1081 cggatccctc gctgaaggcg ctggagtcct cgctcgacaa ggagcagccc gacccggaag
     1141 gcgaaccatg ccccgcttcg tactatgacg cggtcacgga ggagtacaag atgtacacct
     1201 acttctacga gttctggcgc gagggagcca ccggcatcag catcgactag gtggagaggg
     1261 accggaactc catcggccga agtgaactct gatagtgtgg ccccccgggc tgtgcggctc
     1321 ggcggggcca ggacgaggtg tgtgaggcag tggccatgag accgggggcg cggcccggcc
     1381 cggtgggggc gagtgggccc ccgaaactca gatcgtcctt ctgggacagc ccggcgggct
     1441 tgctgctcta caacggtgcc caacaagcgg cgcgagtggc gttggtcgcg gtgggcctct
     1501 ccctggtctt cctggggtgg ggggtgctcg gcgggcacct ggtgaacgcg cagttcgcgg
     1561 actcgccgga cacggcgcag ttccggctgt ggtcgacggt gttctgctat ggctgccttc
     1621 tgctcgccgt ctcggtagtg attcgctatc accaggaaga cggctacatg atgagcctgc
     1681 tcgggctggg ggccttcgcg actttcggcg ttcccttcct gctcaacagc atgctcggcg
     1741 cgttgaacgc cgatctgtcg tcccatgcgg gcgccgatgc ggtgaatgac gcggggcgag
     1801 cgtcgggtat cgccatcttc atcctggtgg gcgcgcgatt tctggtttat ggggcgctcc
     1861 gattgatggc ggccgccgcg cacaaggtgg agccggcgcc cgaggggttc gaggtcctgg
     1921 tggagaagtc aggcgggcag gctgcccgtc cggcaccggg tagccctcgc accttcgcgg
     1981 cctgctggga gttgccattc tgcagtgagt atgtgcggtc cacgtgtccc cgcttcgcgg
     2041 aacggaagtc ctgctggaag cgcaagagtg ggtgtcactg tgatgtggat ctgctggggg
     2101 cggcgctggg gctggatgcc gtggagaagg gcctccggtc ggccaatcgc atcacgcgtc
     2161 aggcgttgca ggatgcgtcg gggcgcaaga tcgcctgcgg tgagtgccgg atcttcatcg
     2221 agcaccagcg ccggaagttt cgcctgctct ccgccttcgc tctgccggtg acgatcctgc
     2281 tgcttctggc actccggcct ctgatcgggc agctctgggg cgcggctgtg gtgggcctgg
     2341 cgcgtgtgac gcaggtggtg gccttctcgt cgccgggcac ggagcgggcc acgcagctgg
     2401 tga
//
LOCUS       DAOSPL010000710         2401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44083_length_2401_cov_3.476556, whole genome shotgun sequence.
ACCESSION   DAOSPL010000710 DAOSPL010000000
VERSION     DAOSPL010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44083_length_2401_cov_3.476556"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLQ54_12690"
     CDS             <1..298
                     /locus_tag="PLQ54_12690"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOS94167.1"
                     /translation="YSIDELPQFWTVLRGDMSIVGPRPQVAREVAEYTAALHRRLLVK
                     PGITGLWQVNGRSSLSVEESVRLDLHYVENWSLTSDLAVILKTVRVVLRPQDAY"
     gene            447..1244
                     /locus_tag="PLQ54_12695"
     CDS             447..1244
                     /locus_tag="PLQ54_12695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS94168.1"
                     /translation="MTIPMVSLVTVAHNSASELQHFWRDWPSDAAEWIVVDNASTDGS
                     AEVARALGARVIRLDVNIGFSAANNVGAARAEGRILGFLNPDLTPTRQGIDALAGRVT
                     TENAFVAPQLVNIDGTLQENGRNAPYPLRKLAHMFAPTSRLNHHYVRHVESGLEQVVW
                     VMGAALFMSREVFEQVGGWDDQYFIYYEDSDICLRALHLGVPTFVDGDVRWVHGWARQ
                     TKTARSLWVWKQEFRSAIRFYRRHPDCIVPRRSRSGSLGKVEGTARP"
     gene            1241..>2401
                     /locus_tag="PLQ54_12700"
     CDS             1241..>2401
                     /locus_tag="PLQ54_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94169.1"
                     /translation="MSGIALGQPRGQTAIPGSNLSREADPLLRLAMASATAAATLPFA
                     IAALGAVEASPVAYGSLVVWQLRGMIYGPLIMLVVTVSAARFQVGKATYWAWSLVFLG
                     YAPAWQISRQRFPWRGSFAVEDIERGQTLILAGHVAFAVVAWLVSRRPPRERHAGLSA
                     LSVQNPEAIKERQRRVLSYLGLGYLLPSMLFIALMGSALFNARAIFRARVLEISSLPF
                     GGFLYFAVTAGAIVIPGALLACQLHAVKVPGWLVAGCWLAAAVVTNPLVGSRFLTGSF
                     LVATAVAVLCYSNLLRLVPAGSVALLMVVFPSLDVLRGDDTGSRGIQLLSVQDSMLDY
                     DFDAFEMGVREVSLDSGQRAGLPPSAQMLIAPVARWIPIAARSFEGNAGGQVV"
BASE COUNT          405 a          786 c          763 g          447 t
ORIGIN      
        1 gtactcaatt gacgaacttc cccagttctg gacggtgctg cgaggagaca tgagcattgt
       61 cggtccccgc cctcaggtgg cccgagaagt ggcggagtac accgcggcac tacatcggcg
      121 cctgctggtg aagccgggga tcaccggcct gtggcaggtg aacggccgat ccagcctcag
      181 tgtcgaggag agtgtccggc tcgacctgca ctatgtcgag aactggtcct tgaccagcga
      241 cctcgccgtg atcctcaaga ccgtgagggt cgtcctccga ccgcaggacg catactgaga
      301 gccctcacga cctgcgacgc gaacgcttcg ccccttgacc gccaggagag tgagcgcgct
      361 tgtcgcgccc tgccagcccc acgcccggac caggtgagtg ggcgaccagg cgccgccaca
      421 caaaccacgc ccccgaaagc aggcccatga caataccaat ggtttcgctg gtgacggtgg
      481 cccacaatag cgcctcggaa ctgcagcatt tctggcgcga ctggccgtcg gatgcagccg
      541 aatggatcgt tgtcgacaac gcgtcaacgg acggatctgc agaggtggca cgtgcccttg
      601 gcgcacgagt catccgactc gacgtcaata ttggcttctc agccgcaaac aacgtgggcg
      661 ccgcccgagc cgagggacga atcctggggt tcctcaatcc cgacctaacc ccgactcgcc
      721 aaggaatcga cgccttggcc gggagagtta ccaccgagaa cgccttcgtg gcgcctcaac
      781 tcgtcaacat cgacggcacg ctgcaggaga acggccggaa cgccccgtac cccctacgga
      841 agctcgccca catgttcgct ccaacttcaa ggttgaacca ccattacgtg cgacacgttg
      901 agagtggact cgagcaagtc gtgtgggtta tgggagcagc cttgttcatg agcagggagg
      961 tctttgaaca ggtcggcggg tgggatgacc agtacttcat ctactacgaa gactccgaca
     1021 tctgcctgcg ggccctccac ctaggggttc ctactttcgt tgacggcgac gtgcggtggg
     1081 tgcacggctg ggcgcgacag accaagaccg caagatcgct ctgggtctgg aagcaggagt
     1141 tcaggtcagc aattcggttc taccgccgcc atcccgattg catcgtgcct cgaaggtccc
     1201 gtagcggaag cctgggtaaa gtggaaggga cggcacgccc atgagcggta tcgccctcgg
     1261 ccaaccccgt gggcagaccg ctataccggg cagcaacctc tcgcgagagg cagacccgtt
     1321 gctgcgcttg gccatggcca gcgccacggc agccgcgacg ctcccgttcg ccatcgcggc
     1381 gctgggggct gtcgaagcga gccccgtcgc ctacgggagc ctcgtcgtgt ggcagttgcg
     1441 gggcatgatc tacggtcccc tcatcatgct ggtggtcacg gtgtccgccg cgaggttcca
     1501 ggtgggcaaa gcgacgtact gggcttggag cctggtcttc ctcgggtacg caccggcctg
     1561 gcaaatcagc cgccagcggt tcccgtggcg aggaagcttt gcggtcgagg acatcgagag
     1621 gggccagacc ctgatcctgg ccgggcacgt cgcgttcgcg gttgttgctt ggctcgtgag
     1681 ccggcgcccg ccacgtgagc gccacgccgg cctttcagcc ctgtccgtgc agaatcccga
     1741 ggcaatcaag gagcggcagc gccgtgtcct ttcgtacttg ggactcggtt acctcctccc
     1801 gtcgatgctt ttcatcgcct tgatgggtag tgccctgttc aacgctcgtg caatattcag
     1861 ggcccgggtc ctggagatct catcccttcc cttcgggggc ttcctctact tcgcggtgac
     1921 ggctggtgca atcgtcatcc cgggggcact cctcgcctgt cagctgcacg ccgtcaaggt
     1981 gccgggctgg ctggtcgccg gatgctggct ggctgcggcc gtagtaacca accccttagt
     2041 gggctcgcgc ttcctcaccg gaagcttcct cgtcgctaca gccgtagccg tgctgtgcta
     2101 cagcaacctc ctccgactcg tcccggccgg cagcgtggcg ctcctcatgg tggtgtttcc
     2161 ttcgctcgac gtgttgcggg gcgacgacac cggctctcgg ggcatccagc tgctctcggt
     2221 gcaggactcc atgctcgact acgacttcga tgccttcgag atgggcgtcc gcgaggtatc
     2281 cctcgattcg gggcagcgag cggggctgcc gccatccgcc cagatgctca tcgccccggt
     2341 cgcgcggtgg atacctatcg ccgcgcggtc gttcgaggga aatgcgggcg ggcaggtcgt
     2401 c
//
LOCUS       DAOSPL010000711         2400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44106_length_2400_cov_2.846482, whole genome shotgun sequence.
ACCESSION   DAOSPL010000711 DAOSPL010000000
VERSION     DAOSPL010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44106_length_2400_cov_2.846482"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLQ54_12705"
     CDS             complement(<1..202)
                     /locus_tag="PLQ54_12705"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOS94170.1"
                     /translation="MDLTIVGVGCKKCDQLESLTRQALADLGIADTPIHRVQDTNDIA
                     DMGVLLTPVLVANGMMVTMGRVP"
     gene            complement(245..1939)
                     /locus_tag="PLQ54_12710"
     CDS             complement(245..1939)
                     /locus_tag="PLQ54_12710"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOS94171.1"
                     /translation="MTLAQLVPPEDLRGIQESFEAAVGVPMLFTDESGRPILDPPSSP
                     LRYCAAFLSGVGGEDRPCLACGRRHDLTESIVSLYEAASSSHTPIVHQCPGGFLDAAV
                     PIRLYGKTIGYAVFARVLDRPADLEALRRMAAEHGLDVEACLEAVRQAPVLPHERVEA
                     LAAHLDKLTGFVIGVAADHYHMQQELALAGEIQRGLIPDWFHVDGFEVALQFQPCSAT
                     GGDLLGAFRLGGGDSILYVGDVSGHGAPAALLMGHLSGALAGLRHANVWPAETLERLN
                     DGFVRHFGAYGSFATLFCARYVASERTLLYANAGHEPPLLLRADGGKIEEVSEPRGCP
                     LGFVAHGRYAHGTVSLEPGDTLLLYTDGLIERKNQAGEWADAGGLMRAVAEIQGQSAF
                     AIRDALAARMADAAWAPPSDDAAFLVARRQRETEVVFGSNAAEADENCGRIVDDLRGR
                     GLAPGDLDAIRLALDEIITNALKHGNALDPSKRIRVHYAVSRAQAKFRVTDEGAGIWH
                     AGLKPLTREPFAGSGYGLALVRGVVDHVEWVPGGTEVFFAKYRTDAPQVVAGHKPC"
     gene            complement(2031..>2400)
                     /locus_tag="PLQ54_12715"
     CDS             complement(2031..>2400)
                     /locus_tag="PLQ54_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94172.1"
                     /translation="RRVINGVVHRNSVLDIAALLDHAEMLGLTEDQITALSARRKECE
                     TACDRAGQAIAAAEARLGTPERDAELERLRRTQLADHARRVLAALDLLSPEQRRKADE
                     WLAATDLQSLVGTAEAAQPR"
BASE COUNT          382 a          785 c          850 g          383 t
ORIGIN      
        1 tgggcactct gcccatggtg accatcatgc cgttcgccac gaggacgggg gtcaggagca
       61 cgcccatgtc cgcgatgtcg ttggtgtcct gcacccgatg gatgggggtg tcagcgattc
      121 cgaggtcggc cagtgcttgc cgggtgagcg actcgagctg gtcgcacttc ttgcagccga
      181 cacccacgat ggtgagatcc aaactgcagc cccccacacg ccagactggt gcgccgccag
      241 ggtatcagca tggcttgtgc cctgcaacca cttggggggc gtcggtccgg tacttggcga
      301 agaacacctc ggtgccgccc gggacccatt cgacgtggtc gaccactccc cgcacgaggg
      361 cgaggccata gccggatccc gcgaacggct cgcgggtcag gggcttgagg ccggcatgcc
      421 agattccggc cccttcgtcg gtgacgcgga atttggcctg ggccctggag acggcgtagt
      481 ggacacggat ccgcttgctc gggtcgagag cattgccatg tttcagggca ttggtgatga
      541 tctcgtcgag tgcgagacgt atggcatcca gatcgccggg agccagacca cggccccgga
      601 ggtcatcgac gatgcggccg caattctcat cggcctcggc ggcgttgctg ccgaagacga
      661 cctcggtctc ccgctgccgc ctggcgacca ggaatgcggc gtcgtcagag gggggcgccc
      721 aggccgcatc ggccatacgt gccgccagcg cgtcgcggat ggcgaaggcc gactggccct
      781 ggatctccgc aactgcgcgc atcagccccc ccgcatcggc ccattcgccc gcctggttct
      841 tccgctcgat gaggccatcg gtgtagagca gcagcgtatc gccgggctcc agactcaccg
      901 tgccgtgtgc gtaccggccg tgggctacaa accccaaggg acacccgcgt ggctccgaga
      961 cctcctcgat tttgccgccg tcggcccgca gcagaagcgg gggctcgtgc ccggcgttgg
     1021 cgtagaggag tgttcgctcc gacgcgacat agcgcgcgca gaacagggtg gcgaaactgc
     1081 cgtaagcccc gaagtgccgg acgaacccgt cgttgagccg ttcgagcgtc tcggctggcc
     1141 acacgttggc gtgccgcagg cctgccagcg ccccggagag gtgccccatg agcagggcgg
     1201 ctggagcacc gtggcccgac acgtcgccga catagaggat tgaatcgcca cctcccaggc
     1261 ggaatgctcc caggaggtca ccccctgtgg ccgagcacgg ctggaactgg agagccacct
     1321 cgaagccgtc aacatggaac cagtcgggga tgagaccgcg ctggatctcg cctgcgaggg
     1381 ccaactcctg ctgcatgtgg taatggtcgg cggccacccc aatgacaaac cccgtcagct
     1441 tgtcgaggtg agcggccagg gcctcgacac gttcgtgggg cagaacaggg gcttggcgca
     1501 cggcttcgag gcaggcctca acatccagac cgtgctcggc ggccatccgg cggagggcct
     1561 ccaggtcggc cgggcggtcc aggacccggg cgaagaccgc gtagccgatg gtcttgccgt
     1621 agaggcggat cggcaccgcc gcatcgagga accctccggg gcattggtgg acgatggggg
     1681 tatgggacga gctggcggcc tcgtacaggc tcacgatgga ctccgtcagg tcgtggcgac
     1741 ggccgcaggc caggcatggc cggtcctcgc ctcccacacc ggagaggaag gcggcgcagt
     1801 agcgcagcgg tgaactcgga gggtccagga tcggacggcc cgattcgtcg gtgaacagca
     1861 tggggacgcc cacagcggcc tcgaagctct cctggatgcc tctgaggtcc tcggggggga
     1921 ccagttgggc gagagtcacg tcaggctccc tcattcgatg aagggtcggg taccatcccc
     1981 tagtcgaacc ccaggagccg tctggtggtt cccggtgggc cccgaggccg ctatcggggt
     2041 tgcgctgctt ccgcagtacc caccagggac tgaaggtcag tggccgcgag ccactcatcg
     2101 gccttgcgcc gctgttcggg gctcaggagg tcgagggcgg cgagtacccg gcgtgcgtga
     2161 tcggccagtt gcgttcggcg gagccgctcg agctcggcgt ctcgctctgg ggtgccgagg
     2221 cgcgcctcgg cggccgcgat ggcctgcccc gctcgatcac aggcggtctc acactctttt
     2281 cggcgggcgg acagcgcggt gatctggtcc tcggtgagcc ccagcatctc ggcatggtcg
     2341 aggagggccg cgatgtcgag gaccgagttc cggtggacca cgccgttgat gacgcgccgg
//
LOCUS       DAOSPL010000712         2400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44109_length_2400_cov_2.705330, whole genome shotgun sequence.
ACCESSION   DAOSPL010000712 DAOSPL010000000
VERSION     DAOSPL010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44109_length_2400_cov_2.705330"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLQ54_12720"
     CDS             complement(<1..212)
                     /locus_tag="PLQ54_12720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS94173.1"
                     /translation="MRSVGSGTRASCPARRWLLVRGRPRTVDRLQQERGGCAVRRSRG
                     FTLIELLVVIAIIAILAAILFPVFAKA"
     gene            complement(209..439)
                     /locus_tag="PLQ54_12725"
     CDS             complement(209..439)
                     /locus_tag="PLQ54_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94174.1"
                     /translation="MKVKGLLVRSIRIGLVTAVMLGLVASAVSCKGNTQGTKAVPKQM
                     TPEQFKQGMDMSKMQKGAGGSPTAPAAESGGQ"
     gene            697..1425
                     /gene="phoU"
                     /locus_tag="PLQ54_12730"
     CDS             697..1425
                     /gene="phoU"
                     /locus_tag="PLQ54_12730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOS94175.1"
                     /translation="MDHPRATFDQELEDLRRSALEMGAFVSGMLDDALKSLFDRNTDL
                     ANELRERDALADEMDEAIESRATRLLALQQPVAGDLRVVTASLKVVTDLERMGDYAVA
                     VARTTLALASEPPAECPPAMREISRLAQTIVRESLQAFEAGDVEFARRVRQSDKAIDR
                     LWHELEADLVSWMTREPARAPQAARYLLVARYLERIGDHAKNVCERVAYMVTGRRKPW
                     RTSSQPGAGPAPDPPAAHREAEGG"
     gene            1422..2285
                     /locus_tag="PLQ54_12735"
     CDS             1422..2285
                     /locus_tag="PLQ54_12735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOS94176.1"
                     /translation="MSKGLGHSTRAVSASWLAAAIVVAMALAGCGGRGGRRALVLAGS
                     TSVQPVAELIAEHYEVEHPHHSVNVQGGGSTAGVRAALEGAADIGMSSRALKPDEVGL
                     EPIVVARDAVVLIVHPSNRTTDLTPEQVRAIYTGRITHWSDVTGPKWSSDDRRDDIIT
                     LVTREEGSGTRSAFEEGVMAKADITPRALVQDSTGTVRAIVASDPNAIGYISLGMVSS
                     EVRALTYGGVQATEETVEEGTYALARPFLLLTRGAPSGEAGQFIRFALSDEGQQLVVR
                     AGYLPVRRLDP"
BASE COUNT          394 a          784 c          826 g          396 t
ORIGIN      
        1 gccttggcga acacggggaa caggatcgcc gccagaatgg cgatgatcgc gatgacgact
       61 agaagttcga tgagcgtgaa ccctcgtgac ctacgcaccg cacatcctcc tctttcttgc
      121 tgaagccggt cgactgtgcg cggccgcccc cgcacaagca gccaccgcct cgcggggcaa
      181 gatgctcggg tccccgagcc aacgctcctc actgccctcc ggactctgcc gcgggagcgg
      241 tcgggcttcc ccccgcgccc ttctgcatct tcgacatgtc catgccttgc ttgaactgct
      301 ccggggtcat ctgtttgggt acggctttgg ttccctgtgt gttccccttg cagctcaccg
      361 ccgaggctac caggcccagc atgacggcgg tgacaagacc gatacgaatg gatcgaacca
      421 gcaatccctt taccttcacc acacacctcc ctcaaagagc ttggccccgc ctcgtggatg
      481 aggacatggc cccctggatc cagcccccac tcgccctcga ccatcacacc cgcccgcgac
      541 ggccgacgcg cgcgggccca gtggaggtgc attctctctg gaatgcctag catcctagtg
      601 atgttactga ggtttttggc tcagctcgtg cggaagggtc gaacgaggga cgatccgaag
      661 aggatttatc atggtctcca tgagggggct ggggcgatgg accacccgcg cgccactttc
      721 gaccaggaac tcgaggatct ccgccgctcg gctctcgaga tgggcgcgtt cgtgagcggc
      781 atgctggacg acgcgctgaa gtcgctgttc gaccgcaaca cggacttggc caacgagctc
      841 cgggaacggg acgcgttggc cgatgagatg gacgaggcga tcgagagtcg ggcgacgcgg
      901 ctgctggcgc tccagcagcc ggtggcagga gacttgcgcg tggtgaccgc gtcgctcaaa
      961 gtcgtgacgg acctcgagcg gatgggagac tatgcggtgg ccgtggcgcg gaccaccctg
     1021 gcgctggcat ccgagccacc ggcggagtgc cccccggcga tgcgcgagat ctcacgcctg
     1081 gcacagacca tcgtgcgaga gtcgctccag gccttcgagg cgggcgatgt ggagttcgcg
     1141 cggcgggtgc gccagagtga caaggccatc gacaggctgt ggcatgagct cgaggccgat
     1201 ctggtgagct ggatgacgcg tgagccggct cgggcacccc aggctgcacg gtatctactc
     1261 gtggcacgct acctggagcg gatcggcgac cacgcgaaga acgtgtgcga gcgcgtcgcc
     1321 tacatggtga ccgggcggcg caagccctgg cggacgtcga gccagccggg agctggcccc
     1381 gcccccgacc cgccggcggc acacagggaa gccgagggcg gatgagcaag gggttgggtc
     1441 acagcacgag ggcggtgtcc gcgtcatggc tcgcagcggc cattgtcgtg gccatggcgc
     1501 tcgcgggctg cgggggccga gggggccggc gggccctggt tctggctggc tcgacctcgg
     1561 tgcagcccgt cgcggagctc atcgccgagc actatgaggt agagcacccg caccacagcg
     1621 tcaacgtaca gggcggggga agcacggccg gcgtgcgtgc cgcactggaa ggtgccgccg
     1681 acatcggaat gtcgtcgcgc gcgctgaagc ccgatgaggt ggggctcgag cccatcgtgg
     1741 tggctcgcga cgccgtcgtg ctcatcgtgc acccgagtaa ccggaccacc gatctgactc
     1801 ccgagcaggt ccgggccatc tacacgggcc gcatcaccca ctggtcggat gtgaccggcc
     1861 cgaagtggtc gagcgacgat cgccgtgatg acatcatcac gctggtcacg cgtgaggagg
     1921 gttccggcac ccgaagcgcg ttcgaggagg gggtcatggc caaggccgat atcacgccac
     1981 gcgctctggt ccaggactcc acaggcacgg tccgcgctat cgtggcatcc gaccccaacg
     2041 ccatcggcta catctcgctg ggcatggtgt cctccgaggt gcgtgctctg acgtacggcg
     2101 gtgtccaggc gaccgaggag accgtggagg agggcactta cgcactcgcg cgtccgttcc
     2161 tgctcctgac gcgcggtgcg ccttcgggtg aagccggtca gttcatacgc ttcgcgctga
     2221 gcgacgaggg gcagcagctg gtcgtgcggg ccgggtacct tccggtcagg aggcttgacc
     2281 catgacggta gctcggcgcg agaaggcgat cgagcgcgcc ctattggtct cggcgctctc
     2341 ggccgtgtcg atgctggcgc tcattgcggc cttcatcctc atcgagggcg cccccgtgat
//
LOCUS       DAOSPL010000713         2399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44133_length_2399_cov_1.793515, whole genome shotgun sequence.
ACCESSION   DAOSPL010000713 DAOSPL010000000
VERSION     DAOSPL010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44133_length_2399_cov_1.793515"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLQ54_12740"
     CDS             complement(<1..283)
                     /locus_tag="PLQ54_12740"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097300.1"
                     /note="regulates pyrimidine biosynthesis by binding to the
                     mRNA of the pyr genes, also has been shown to have uracil
                     phosphoribosyltransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase"
                     /protein_id="HOS94177.1"
                     /translation="MSTRTVLQEADIARALTRISHEILESNRGAEGLVILGIPTRGVE
                     LGQRIAALIAGISGVEVPVGALDVTMYRDDLGRNPTRTPRPTSIPDGGID"
     gene            complement(405..962)
                     /locus_tag="PLQ54_12745"
     CDS             complement(405..962)
                     /locus_tag="PLQ54_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOS94178.1"
                     /translation="MRRGRIFRDGIGGPVRYTIGELDMVADFPKAKLREYAGEETAYW
                     FTIPADLVSTNIRDGEIDWSNSIFLSMQFQTGRVGKFNEFMYTFMKCLSRDRIEYVEN
                     WYAEQSDTAEDIQLDDWVMQRRCPHLRADLSKTGKVENGVLTCSLHDWKWDLATGKCL
                     TSHGHPVRAEKAGDDLTRDALTPSA"
     gene            1041..>2399
                     /locus_tag="PLQ54_12750"
     CDS             1041..>2399
                     /locus_tag="PLQ54_12750"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS94179.1"
                     /translation="MVKEYKDLVCTPLGIVRDANFFRNVKNVTVHTSCLATRIDRERK
                     VVKATNTATGEECLFPYDELVLATGASPIRPPIPGVDLNRVFTLWTMPDAMAMKSAID
                     AGSVKSAVIIGGGLIGMEVVEALADRGISVSVADILPTPLPALIGEEFGLRVANVLKQ
                     KGVGFYGGEKVIEIVGEGGLVKAVKTDKRELPADMVLMAVGIRPNTALAREAGLDLHA
                     NGTIMVDECLRTSDPFIFAGGDCVQSKNILTEKSVWQPMGSTANRQGRVIADNIAGLP
                     SRFAGVVGTAILKLFHLTIGKTGLNEEQGRAAGFDPVSVIVEEPDRPHFMPGMGAMTI
                     RLVADRRSRKVIGAQFMGNGVVDKRLDSIVAAISSGMTVDMLADADFAYAPPFATALD
                     PTTHAANTLRNKMDGLVKTYSPAELKARMEEAEPPVLLDVRSPQELDQQGRLPYDFIN
                     IPL"
BASE COUNT          421 a          804 c          769 g          405 t
ORIGIN      
        1 catcgatgcc accatcgggg atgctcgtgg ggcggggcgt gcgcgtggga ttgcggccga
       61 ggtcatcgcg gtacatcgtc acatcgagtg ccccaacggg cacctcgacg ccggagatgc
      121 cggcgatcaa cgcagcgata cgctggccaa gctccacgcc gcgggtcgga ataccgagga
      181 tcacgaggcc ctctgctccc cggttcgact caaggatctc gtgcgagatc cgagtcaacg
      241 cccgggcaat gtcggcttct tgcaacacgg ttcgcgtgct cattcgccgc tcccttctcc
      301 gtctctctga acggtcttaa aggttgctgt acacactcat cctagccgag gcgctcggtg
      361 gcttcgtgcc tgtcccgcgc ctgcgcctgc ccgacgacgg gcaactacgc cgaaggcgtc
      421 agcgcatccc gagtcagatc gtcgccagcc ttctccgcgc gcacaggatg cccgtggctt
      481 gtgaggcact tgccggtcgc gagatcccac ttccagtcgt gcaggctgca ggtgagcacg
      541 ccgttctcga ccttgccggt cttcgagaga tcggcccgaa ggtgcgggca acgacgctgc
      601 atcacccagt cgtcaagctg aatgtcttcg gcggtgtcgg actgctctgc gtaccagttc
      661 tcgacgtact cgatgcggtc gcgcgagagg cacttcataa acgtgtacat gaactcgttg
      721 aacttgccaa cgcgtccggt ctggaactgc atcgagagga agatcgagtt tgaccagtca
      781 atctcgccat cgcggatgtt ggtcgagacg aggtcggccg gaatcgtgaa ccaatacgcc
      841 gtctcctccc ccgcgtattc gcgcagcttg gccttcggaa agtcggccac catgtcgagc
      901 tcgccgatgg tgtagcgcac cggcccaccg atcccgtcgc ggaagatgcg accgcgacgc
      961 atcagcggct cccaccacgc cttgagcgcg gcgagcatct cagcgtcgcc ggatgcggct
     1021 tcccctactt cgtgggcgac gtggtcaagg agtacaagga cctggtctgc actccgctgg
     1081 gcatagtgcg cgacgccaac ttcttccgca acgtgaagaa cgtcaccgtc cacacatcct
     1141 gcctggccac ccggatcgac cgcgagcgca aggtggtcaa ggcgacgaac accgccaccg
     1201 gcgaggagtg cctgttcccc tacgacgaac tcgtgctggc caccggcgcg tcccccatcc
     1261 gcccgccgat ccccggcgtt gatctgaacc gcgtgttcac cctgtggacc atgccggacg
     1321 cgatggcgat gaagtccgcg atcgacgcgg gcagcgtcaa gtcggccgtg atcatcggcg
     1381 gcggcctgat cggcatggag gtcgtcgagg ctctggccga ccggggcatc tcggtctccg
     1441 tggcggacat tctgcccact ccgctgcccg ccctgatagg cgaggagttc ggcctgaggg
     1501 tggccaacgt gctgaagcag aagggcgtgg gcttctacgg cggcgagaag gtgatcgaga
     1561 tcgtcggcga gggaggccta gtcaaggctg tcaagaccga caagcgcgag ctgcccgcgg
     1621 acatggtgct gatggcggtc ggaatccgtc ccaacaccgc gctggccagg gaggcggggc
     1681 tcgacctgca cgccaacgga accatcatgg tcgacgagtg tcttcgcacc agcgacccgt
     1741 tcatcttcgc ggggggcgac tgcgtccagt ccaagaacat cctgacggag aagtccgtgt
     1801 ggcagccgat gggctccacc gctaaccgcc aggggcgcgt gatagcggac aacatcgccg
     1861 ggctcccgtc gcgcttcgcc ggggtcgtcg gcaccgcaat cctgaagctg ttccacctga
     1921 ccatcggcaa gaccgggctc aacgaggagc aggggcgcgc cgccgggttc gacccggtca
     1981 gcgtgatcgt cgaggagccg gaccggccgc acttcatgcc ggggatgggg gcgatgacca
     2041 tacgcctcgt cgccgacagg aggtcgcgca aggtgatagg cgcccagttc atgggcaacg
     2101 gtgtggtcga caagaggctg gactccatcg tggcggccat ctcctccggc atgaccgtgg
     2161 acatgctggc ggacgccgac ttcgcctacg cgccgccctt cgccacggct ctcgacccga
     2221 ccacgcacgc ggccaacacc ctcaggaaca agatggacgg cctggtgaag acctactccc
     2281 ccgcggagct caaggcccgc atggaggagg ccgagccgcc cgtgctgctg gatgtgcgct
     2341 cccctcagga gcttgaccag cagggacgcc tgccctacga cttcatcaac attccgctg
//
LOCUS       DAOSPL010000714         2398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44143_length_2398_cov_3.294067, whole genome shotgun sequence.
ACCESSION   DAOSPL010000714 DAOSPL010000000
VERSION     DAOSPL010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44143_length_2398_cov_3.294067"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PLQ54_12755"
     CDS             complement(<1..847)
                     /locus_tag="PLQ54_12755"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS94180.1"
                     /translation="MRQVPSFSLGPALMAAAVIAMAHAGHAVAAQGANVLKIREDFAA
                     YAVGSDGAPRWVPQTRDWRMEGGAYVHSQPLRDGSCTWLGEASFSDLILTARFRIEPE
                     GQGVRAAGLVFRSVSSEDQYYAHFDSRNAQVILVRQSRTEPWHELARVRGVPIATGVW
                     HDAKVECVGDRITAYLDGRRVAEATDGVFAAGRIGLRAGQGRISFDRVRVQGTPQPQT
                     EEWVLLPQSHPNDELDVPRLRAAERFPAVRGGGYFPVLLKLADGRLGAVVRGGAPHVG
                     LAGRLD"
     gene            complement(863..1960)
                     /locus_tag="PLQ54_12760"
     CDS             complement(863..1960)
                     /locus_tag="PLQ54_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOS94181.1"
                     /translation="MVRQEVEAKLGRVRSFLDEQRLAALVLQSPPNVAWITGGADIHI
                     QMAAESGAATVLVTPDRQCVVCSNIERPRLLAEELAGDEFELVDFAWHEADVSSVLRD
                     LVPGGRVASDSGIMGSLQIPGQVHRLQAPLLPEELARYRALGGASGRALGEAMHAVEP
                     GMSEHDVAARIAERALAAGVLPSLVLVAADERIWRYRHPIPTAARVTRHVMGVLVGRR
                     WGLHSSQTRVVHFGAVPADLARRHAACVRVDAAMILSTVPGAPVAGIIRAALQAYSDA
                     GFPDEWRLHHQGGATGYAARTYKGTPSSSEVVLENEGFAWNPSITGAKSEDTVLAQPG
                     GPEVLSESPGWPMAPVEWGGRSIVRPDILVR"
     gene            complement(2094..>2398)
                     /locus_tag="PLQ54_12765"
     CDS             complement(2094..>2398)
                     /locus_tag="PLQ54_12765"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOS94182.1"
                     /translation="RTDVLEFTEMVTIMGVNPGFPGQAFVPSVLRKIADLRAMIDRQG
                     LDVRIQVDGGMRVDLAESVRQAGADILVAGASVFAPGTDYGETIRRLRGESALTVA"
BASE COUNT          413 a          857 c          793 g          335 t
ORIGIN      
        1 agtccagtcg gccggccagc cctacatgcg gagctccccc ccggactacg gctcccagcc
       61 ggccatcagc aagcttgagc aggaccggga agtagccccc gccgcgcacg gcagggaaac
      121 gctcggcggc acgcaaacgc ggcacgtcca gttcgtcatt gggatgcgac tgcggcaaga
      181 gaacccactc ctccgtctgg ggctggggtg tgccctgcac tctgacgcgg tcgaacgaga
      241 tgcgtccctg tccggcccgc agcccgattc ggccggcggc gaacacgcca tccgtcgcct
      301 cggcaacgcg tctgccgtcc aggtaggccg tgatgcggtc gcccacgcac tcgaccttcg
      361 cgtcgtgcca cacacccgtc gcgatgggca cgccgcgcac ccgcgccaac tcgtgccacg
      421 gctctgtgcg cgattgccga acgaggatca cctgggcgtt tctgctgtcg aagtgcgcat
      481 agtactggtc ctcgctgctg accgaccgga agacaaggcc agccgcccgt acgccctgcc
      541 cctcgggctc tatccggaac cgagcggtga ggatcaggtc cgaaaaggat gcctcaccaa
      601 gccaggtgca gctcccatcg cggaggggct gggagtgaac ataggcccca ccctccatcc
      661 gccaatcccg ggtctgggga acccagcgcg gcgcgccgtc actaccgacg gcataggcgg
      721 cgaaatcctc ccggatcttc agtacattgg ccccctgagc cgccacggcg tggcccgcat
      781 gtgccatggc gatgacggcc gccgccatca gcgcgggacc gagtgaaaac gagggaactt
      841 gtcgcatcga ggatagcctc cactacctga caaggatgtc cggccgaacg atcgagcgtc
      901 cgccccattc gaccggtgcc atgggccagc cggggctctc cgagagcacc tcgggcccgc
      961 cgggctgggc gaggactgtg tcctcggact tggcgccggt gatggacggg ttccacgcga
     1021 acccctcgtt ctcgagcacc acctcggagg agctgggagt acccttatag gtccgggcgg
     1081 catagcccgt ggccccgcct tgatggtgca gtcgccactc atccggaaac ccggcgtcgg
     1141 agtaggcttg gagagcggca cggatgatgc cggccacggg cgcgccgggc accgtcgaga
     1201 gaatcatggc ggcatcgacg cggacgcacg ccgcgtggcg acgggcgaga tccgcgggca
     1261 cggcgccgaa atggacgaca cgggtctgcg atgagtgaag cccccaccgg cggcccacca
     1321 gcacgcccat gacgtgccgc gtcacgcgcg ccgccgtagg gatcgggtgg cggtagcgcc
     1381 agatccgctc gtcggccgcc accagcacca ggctcggcag cacgccggcc gcaagcgccc
     1441 tctcggcgat ccgagccgcc acgtcgtgtt ccgacattcc cggctcgacc gcgtgcatgg
     1501 cctcgccgag ggcccggccc gaggcccctc ccagcgcgcg atagcgcgcc aactcctcgg
     1561 gcagaagggg ggcctgtagg cgatgaacct gtccggggat ctgcaacgag cccatgatgc
     1621 ccgagtccga ggccacccga ccgcctggca ccagatcacg caacaccgaa gacacgtcgg
     1681 cctcatgcca ggcgaagtcc acgagctcga actcgtcgcc ggcgagttcc tcggccagca
     1741 gccgaggacg ctcaatgttc gagcacacga cgcactggcg gtcgggggtc accagcaccg
     1801 tggcggcccc gctctcggcc gccatctgga tgtggatgtc ggctcccccg gtgatccagg
     1861 ccacattggg tgggctctgg agcaccaggg cggccagtct ctgttcatcg agaaagcttc
     1921 ggactcggcc gagtttcgcc tcgacctcct ggcggaccat ggcgcgcgaa catcccatcg
     1981 gggaggaatc gtacggaaac gggggagcat gagggccccg cagcccaacg ggggttcgcc
     2041 gcgccgccgc tccaacctgc acggcgcggg caggactcac aaacggcgat ggatcaggcc
     2101 acggtgagtg ccgactcgcc gcgcaggcgg cgaatcgtct cgccatagtc cgtcccggga
     2161 gcgaaaacgc tggcgccagc cacgaggatg tctgcgccgg cctggcggac ggattcggcc
     2221 aggtcgaccc tcatcccgcc atcgacttgg atccggacgt ccaggccctg ccggtcgatc
     2281 atcgcccgga ggtcggcgat cttgcggaga accgagggca caaacgcttg gcccgggaaa
     2341 ccggggttca ctcccatgat cgtgaccatc tcggtgaact ccagtacgtc ggtccgct
//
LOCUS       DAOSPL010000715         2397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44172_length_2397_cov_3.388130, whole genome shotgun sequence.
ACCESSION   DAOSPL010000715 DAOSPL010000000
VERSION     DAOSPL010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44172_length_2397_cov_3.388130"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PLQ54_12770"
     CDS             <1..955
                     /locus_tag="PLQ54_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94183.1"
                     /translation="YVAYVGGVPVHSGSPASRIMVRDLGRPNDPVNVSADLHAARAPA
                     WSPAGRLAFEAVDGSSSDIYVCRPDGSERLNVTRTPDEWESAPAFVDEGHLVCLSGPD
                     RTVLVLVDVATRQQRRLSEATRFHGAPAVSPTADTVAVFASEHLGDPGDLVLVPVDGG
                     PVRTLSTESALYSAPCYSPDGDAIYACFDGCDIGGTARGLARFPLDGGAPALVADKGY
                     PFSAVSVSPDGSRVAYTTSTAYHDTWISLARADGSGAARLDVTGFHIIGWPAFTSDGR
                     WLAFEGVYAASYSVNVVDLASGVVSRITPEGDTGVRPVASR"
     gene            complement(966..2216)
                     /locus_tag="PLQ54_12775"
     CDS             complement(966..2216)
                     /locus_tag="PLQ54_12775"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOS94184.1"
                     /translation="MLESSAPAVSLRDLFPARQAATDPLEVRVVSERLRRRTRRRLLL
                     REVRFDSHEWQQGTVRIAAFVAVPLGLGPVPAMVVGAGDLKRAADFAARHQIGCLCFD
                     RPGTGASTGPADEYANWIHFDHPREGWMWHYVYAALRALTYLQSLPEVDPTRLGITGY
                     SRGGTMAYIANGVDPRICVSVPQASAGGILGAVDVGGWAASLHRDAAGRPYVPEAFYE
                     FARYYDPALYADMQNGLVMPIIGAQDEFFPLETVAGTVRAMSPRCRLELIANWDHAYF
                     AGGNADVDAFDNRRAFRTRTRRWLAAATRCLREGGNTIPPMPVLQVDERGQELHWEIT
                     AAHHEDMVRGRLWYSTDGAYTFAATRAVRCAAGFVTRLRLSRDVAAGLAAFAEVEYRQ
                     GPLISSLPYLGPAFCQRLRPFPEQ"
BASE COUNT          364 a          836 c          825 g          372 t
ORIGIN      
        1 ctacgtcgcc tatgtcggcg gcgtgcccgt gcactcggga tcgcccgcgt cgcggatcat
       61 ggttcgcgac ctcggccggc cgaacgaccc ggtcaatgtg agtgccgatc tgcacgcggc
      121 acgagctcca gcctggtcgc cggccggtcg gcttgccttc gaggccgtcg atgggtcgtc
      181 atctgacatc tacgtctgcc ggcctgacgg ttcggaacgg ctcaatgtca cccgcacgcc
      241 cgacgaatgg gagtcggcgc ccgcgttcgt cgatgagggg cacctggtct gtctgtccgg
      301 gcccgaccgc acggtgttgg tgctcgtgga cgtcgccacg cggcagcagc ggcggctgtc
      361 cgaggccacg cgcttccacg gcgctccggc cgtgagcccc acggcagaca ccgtcgccgt
      421 gttcgcctcg gagcacctgg gtgacccggg cgacctcgtg ctcgtaccgg tggatggcgg
      481 cccggtgcgg actctcagca ccgagtcggc gctctactcg gcgccgtgct actccccgga
      541 tggcgacgcg atctatgcat gcttcgatgg ctgcgacatc ggcgggaccg cacgcgggtt
      601 ggcccggttt ccgctggacg gcggtgcccc ggccctggtg gcggacaagg gctacccctt
      661 ctcggccgtg agcgtgtctc cggacggcag ccgcgtcgcc tacacgacga gcacggcgta
      721 ccacgacacg tggatcagtc tggcgcgcgc ggatggctcc ggggcggcgc ggctcgatgt
      781 caccggcttc cacatcatcg ggtggcccgc gttcacctcg gacggacgat ggctcgcctt
      841 cgagggcgtc tatgcggcca gctactccgt gaatgtggtg gacctggcgt ccggtgtcgt
      901 gtcccgcatc acgccggagg gggacacggg tgtccggccc gtggcctccc gctaggccct
      961 gcgcgtcact gctcagggaa cggtcggagc cgctggcaaa aagccggccc gaggtacggg
     1021 agcgaggaga tcagcgggcc ctggcggtac tcgacctcgg caaaggcagc cagaccagcc
     1081 gcgacatcgc gtgagagacg cagccgcgtc acgaagccgg ccgcacaacg aactgcccgc
     1141 gtcgccgcga aggtgtacgc gccgtcggtc gagtaccaga gcctcccccg caccatgtct
     1201 tcgtggtggg cggccgtgat ctcccaatgc agttcctggc cccgctcgtc cacctgcagc
     1261 accggcatgg gtgggatggt gttcccgcct tcacgcagac agcgggtagc ggccgccagc
     1321 caacggcgcg ttcgcgtccg gaacgctcgc cggttatcga aggcgtcaac atccgcattc
     1381 ccacccgcga agtaagcatg gtcccagttc gcaatgagct ccagccgaca gcgaggcgac
     1441 atggctcgaa ccgtgcccgc caccgtttcc agggggaaga actcgtcctg cgcgccgatg
     1501 atgggcatga cgaggccgtt ttgcatgtcc gcatacagcg ccgggtcgta gtatctcgcg
     1561 aactcgtaga aggcttcggg gacgtacggc cggcctgcag catcgcggtg gaggctcgcc
     1621 gcccagcccc cgacatccac ggcccccagg ataccgccgg cggacgcctg gggcaccgag
     1681 acgcatatcc gggggtcgac accgttggcg atgtaggcca tggtgccccc gcgcgagtag
     1741 ccggtgatgc ccagccgcgt tgggtcgacc tcgggcaacg actgcaggta ggtcagagcg
     1801 cgcagcgcgg cgtagacgta gtgccacatc cacccctcgc gggggtggtc gaagtggatc
     1861 cagttggcgt actcgtccgc cggtcccgtg ctggcaccgg tcccgggccg gtcgaagcac
     1921 agacagccga tctgatgccg tgcggcgaag tcggccgcac gcttcaggtc accggcgccg
     1981 accaccatgg cgggtacggg acccaacccg aggggcactg ccacaaatgc cgcgatgcgg
     2041 accgtgccct gctgccattc gtgagagtcg aaccgcactt ccctcaggag cagccgtctg
     2101 cgcgtccgac gccgcagccg ttccgacacc acgcggacct cgagcgggtc ggtggcagcc
     2161 tgcctcgcgg gaaacagatc ccgcagcgaa actgcgggag cggatgactc gagcatacta
     2221 agcctttcct ttgcggcccg gccatacctc cggtcggcta cggggacaag cagggccatt
     2281 ggcggtaact gagggctccc gtggcccatc agcctggttg cggaggggcg ccatgggggg
     2341 ggaccacgcg ttctcccagc gtggtgggcc cccaacgcgc ccgatccgca cgcctca
//
LOCUS       DAOSPL010000716         2396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44197_length_2396_cov_3.126869, whole genome shotgun sequence.
ACCESSION   DAOSPL010000716 DAOSPL010000000
VERSION     DAOSPL010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44197_length_2396_cov_3.126869"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PLQ54_12780"
     CDS             <1..718
                     /locus_tag="PLQ54_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS94185.1"
                     /translation="GRMPLLAEFGCGGSRPRGREAQERGMDIAEIKGFDDPRLPDWLD
                     IWQAAFPAEELVPFSRVLCELGRSGSPHHFVAGSIDDRVCAMAWYVDMAGGTDHGAIW
                     LWYLAVHWEMRGRGHGAALLRDLVDRFRRRGTVRALVLEVEDPDALKGPRSDLAWRRI
                     GFYQRLGCGLLTGVSYSIRAAAWSPRTRMCWMVLPFAPLVAEEAFELIRVSTEAEISR
                     VGPLGLEFGEPSVPNQPTRP"
     gene            884..>2396
                     /locus_tag="PLQ54_12785"
     CDS             884..>2396
                     /locus_tag="PLQ54_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006668367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOS94186.1"
                     /translation="MGGAEVDGSRIHERQGDELTEGGRHFRELFARSRDGFVVLDAHG
                     RFLDANPAYCAMVGYSLEELRALPSFLTITPERWHPWETQEILGERQLDGGISKVYEK
                     EYIRKDGTVFPVEVQVYAVRHGDGSLDCLWEIARDITERRRAEQALRESEERWRALFE
                     ASPMGIYLYRLDEAGRLIFEAGNPAADRLTGADSSQFAGKAIEEAFPPLVEAGIPDRY
                     RRVARDGERWHTDEVAYEDERIRGAFEVYAFQIAPNRMAVVFEDITDRKRAEEALRRQ
                     TEEMERYFSRSLDLLCIADTRGRFIRLNPEWERVLGYPVSELEGRAFLDLVHPDDREA
                     TLEAVSRLAAQEDVLSFENRYRCQDGTYRWIEWRSTPVGATIYAAARDVTDRKRLEAA
                     LRESEELQRTIIDASPLAIISVDLERRVRSWNAAAERMFGWTASEVLGRPLPYLGDGN
                     EEWAREAHEMMDRVLAGETLSRVEVARPRKDGTLIHVSLSTAPVRDRSGQVTAL"
BASE COUNT          382 a          709 c          908 g          397 t
ORIGIN      
        1 tgggaggatg cccttgttgg ccgagttcgg ctgtgggggc agcaggcccc gtgggcggga
       61 agctcaggag cgtggcatgg acatcgcgga gatcaaaggg ttcgacgacc cgaggctgcc
      121 ggactggctc gacatctggc aggcagcgtt cccggcggag gaactggtgc cgttctcgcg
      181 ggtgctctgt gaactcggcc gctcgggttc gccgcaccac ttcgtggcgg ggagcatcga
      241 cgaccgggtg tgcgcgatgg cgtggtacgt ggacatggct ggcggtaccg accacggggc
      301 gatctggctt tggtacctgg ccgtccactg ggagatgcgc ggcagaggtc acggggcggc
      361 gcttctccgc gacctggtgg accgcttccg gcgcaggggt acggtgcgcg cgctggtgct
      421 cgaggtggag gacccggacg cactgaaagg gccccgttcg gacctggcgt ggcggcgtat
      481 cggcttctac cagcgactgg gctgcgggct gctcacgggg gtgtcatact cgatccgcgc
      541 cgccgcgtgg tcgcctcgga cccggatgtg ctggatggtc ctcccgttcg cgcctctggt
      601 cgcggaggag gcattcgagt tgatacgagt ttcgacggag gccgagatca gccgagtggg
      661 gccgctggga cttgagtttg gggagccatc agttccaaac cagccgacac ggccgtgact
      721 ggcgaccgac gccgcccgaa gggcgcggcg gcccggtcgg cgaatcacct cgttacggca
      781 ggccggagtt cgttggtggg ctgatctgcg gggggcttcg atggtgggct ctgccggtgc
      841 tatgacaggc ccagcgggcg gattcggtaa gggggtagct ccggtgggtg gtgctgaggt
      901 cgacggcagc aggatccacg aacggcaggg cgacgagctg accgaaggcg ggcggcattt
      961 ccgcgagctg ttcgcacgca gccgagacgg gttcgtagtg ctggacgccc acgggcggtt
     1021 tctggacgcc aatccagcct actgcgccat ggtgggctac tccctcgagg agctgcgggc
     1081 tctcccctct tttctgacca tcaccccgga gcgctggcac ccgtgggaga ctcaggagat
     1141 cctgggcgaa cgccagctcg atggcggcat ttccaaggtg tatgaaaagg aatacatccg
     1201 caaggatggc acggtcttcc cggtcgaggt gcaggtctat gcggtgcgcc atggcgatgg
     1261 tagcctcgac tgcctctggg agatcgcccg ggacatcacc gaacgcaggc gagcggagca
     1321 ggcgctgagg gagagcgaag agcggtggcg cgccttgttt gaggcgtcac cgatgggcat
     1381 ctatctgtac cgcctcgacg aagccggccg actcatcttt gaggcgggca atcccgccgc
     1441 cgaccgactg accggtgccg atagctccca gtttgcgggc aaggccatcg aggaggcatt
     1501 cccgccgcta gtcgaggccg ggatacccga ccgttaccgg cgcgtagcgc gagacggtga
     1561 gcgttggcac accgatgagg tcgcctatga ggacgaacgg atccgcggcg cgttcgaagt
     1621 ctacgcgttc cagatcgccc cgaacagaat ggcggtggtg ttcgaggaca tcacggaccg
     1681 caagcgggcg gaggaggcgc tccgccggca gaccgaggag atggagcgct acttcagccg
     1741 aagcctcgat ctgctgtgca ttgccgacac ccgcggccgg ttcatccggc tgaaccccga
     1801 gtgggagcgc gtgctcggct acccggtgtc cgagctggag ggacgtgcgt tcctggatct
     1861 cgtccacccg gatgaccgcg aggccactct ggaggccgta tcgcggctcg ccgctcagga
     1921 agacgtgctg agctttgaga accggtaccg gtgccaggat ggaacgtacc ggtggatcga
     1981 gtggcggtcg acgccagtcg gggcaaccat atacgccgcg gcgcgcgatg tgacggatcg
     2041 gaagcggttg gaggcggcgc tgcgcgagag tgaggagctg cagcgcacca tcatcgatgc
     2101 gtcgccgctg gctattatca gtgtggactt ggagcggcgg gtccggagct ggaatgcggc
     2161 cgccgagcgc atgttcggtt ggacggcctc cgaagtcctg ggccgtccgc tgccctatct
     2221 gggcgacggc aacgaggagt gggcgcgcga ggcccacgag atgatggatc gcgtcctggc
     2281 cggggagacg ctgtcgcgcg tggaggtcgc gcggccgcga aaggacggca cgctgatcca
     2341 cgtcagcctc tcgacggcgc cggtgcgcga ccggagtggc caggtcacgg cgctga
//
LOCUS       DAOSPL010000717         2394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44257_length_2394_cov_3.390765, whole genome shotgun sequence.
ACCESSION   DAOSPL010000717 DAOSPL010000000
VERSION     DAOSPL010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44257_length_2394_cov_3.390765"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..1173)
                     /locus_tag="PLQ54_12790"
     CDS             complement(67..1173)
                     /locus_tag="PLQ54_12790"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOS94187.1"
                     /translation="MWLSSICQDPEYRWWATTKLVEGKAPPSDPLSWRGLQYQYQTLL
                     LGLRETKSPVSASPPSRPNSKLFANSGYASMHTNLANPADDVVLNVRCAPYPYGAVHH
                     AHPDQNSFVFYYHGEPLAINAGHYGGTANISWDGPYMTYYARQTKSKNAILIDDIGQK
                     SGSATATGEILRFEDAADYTYFVGDAVQAYNDNQKGLANAVKRHVAMIHQAGQRPFVV
                     MLDEVDMPSAHKLKWLLHTMKQATVDEAGQSITLRYDTARARVKLLASEALTISQTDQ
                     TDPPLDPGNTVAANIARLGESWHLTAETSSAKTSFRIGTVLFPYEAGAEGDVPVIDGY
                     TAPNNRFVFVVGANTITLDLSSMSLSIMTGQTPG"
     gene            complement(1206..>2394)
                     /locus_tag="PLQ54_12795"
     CDS             complement(1206..>2394)
                     /locus_tag="PLQ54_12795"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HOS94188.1"
                     /translation="PGSGARGRPPGGEPTGGHHAVDLRVTTPRWLGGTYLDLRMGRDR
                     RSAKRPHTVPTRVRRMNRLLITFAVQMFAAWLCVLGVAFYWATQTACGGGAAILGDSS
                     VDASGVNTGRTIDRGGRANGALANARLQHPKVDLLKSEIQEFRNLKNQGAVYKDAYDW
                     LMTYGDKAVARTTEYAYSQKGSWASDLTTCRYAYLASGDAKYAEHAKALALYAAQNWV
                     PASGVTATRDATAEFARTYDTLYDYLSSAERSILLDATRTWGMLLYSEIQKNYKLLMG
                     PMTANSIGYLAEAALVMDGEFAEASTWMDFCVNHYTGTIDGYSWPWYSAGMTEDLRRA
                     STTGSSDGRGPWESSTRWRNWATRTCTRARPSGTWASRFSICCLPTPRSTARSATADS
                     LAT"
BASE COUNT          394 a          825 c          732 g          443 t
ORIGIN      
        1 cgcgcgagtg ccgcgaggcc tcggccccgc ggcactctgt gaaacccgaa ctcgaagtac
       61 ccggcactag ccaggcgtct gccccgtcat gatcgacagc gacatcgaac tcaggtcgag
      121 cgtgatcgtg ttcgccccca ccacgaacac gaaccggttg ttcggggcag tgtacccgtc
      181 tatcaccggc acatcgccct ccgcacccgc ctcgtaaggg aacagcaccg tcccgatccg
      241 gaatgacgtc ttcgcgctgg atgtctccgc cgtcaggtgc cagctctcgc ccagccgggc
      301 aatgttggcg gccaccgtgt tgcccgggtc gaggggcgga tcggtctggt cggtctgact
      361 gatcgtcagc gcctcggagg ccagcagctt cacccgggct cgcgcggtgt cgtaccggag
      421 tgtgatggac tggccggcct cgtcgaccgt agcctgcttc atcgtgtgga gcagccactt
      481 gagcttgtgg gccgagggca tgtcgacttc gtcgagcatc accacgaagg gccgctggcc
      541 ggcctggtgg atcatcgcca cgtgccgctt caccgcattc gcgaggccct tctggttgtc
      601 gttgtaggcc tgcaccgcgt cccccacgaa gtacgtgtag tccgcggcgt cctcgaagcg
      661 caggatctcg ccggtagccg tcgcgctgcc tgacttctgc ccgatgtcgt cgatcaagat
      721 ggcgttcttc gacttggtct gccgcgcata ataggtcatg tacggcccgt cccagctgat
      781 gttggccgtc ccgccatagt ggccggcgtt gatggccagc ggctcgccgt ggtagtagaa
      841 cacgaacgag ttctggtcgg gatgcgcgtg gtgcacggcc ccatacgggt acggcgcaca
      901 cctcacgttc agaaccacat catcggccgg gttggccagg ttggtgtgca tgctggcata
      961 gccgctgttg gcgaacagct tcgagttcgg ccgagacggc ggcgacgccg acaccggcga
     1021 cttggtctcg cggaggccca gcagcagcgt ctgatactgg tactgcaggc cgcgccagct
     1081 cagcgggtca ctcggcggtg ccttcccctc cacgagcttc gtcgtggccc accaacggta
     1141 ctcggggtcc tgacagatgg agctgagcca catcatctca aagcgctggg caaacgcctc
     1201 cgaaatcagg tggcaagact gtccgccgtc gccgaacgtg ccgtagaacg gggcgttggg
     1261 aggcaacaga tagagaaacg actcgcccat gttccggaag gcctcgcgcg agtgcatgtc
     1321 cgcgtagccc agtttctcca tcttgtcgaa gactcccatg gcccgcgtcc atccgatgac
     1381 ccggtagtag aggccctgcg caagtcctcc gtcatccccg ccgagtacca cggccagcta
     1441 tacccgtcga tggtgcccgt gtagtggttc acgcagaagt ccatccatgt cgaggcctcg
     1501 gcgaactcgc cgtccatcac cagggcggct tcagccagat agccgatgga attcgccgtc
     1561 atggggccca tcagcagttt gtagttcttc tgaatctcgc tgtacagcag catgccccac
     1621 gtgcgcgtcg cgtccagcag tatcgaccgc tccgcgctgc tcaaatagtc gtacagcgtg
     1681 tcgtaggtcc gtgcgaactc cgccgtggca tcgcgcgttg ccgtgacccc gcttgcgggc
     1741 acccagttct gggcggcgta cagggccaat gccttcgcat gctcggcgta cttggcatcg
     1801 cccgaggcca gataggcata ccggcacgtg gtcaggtccg aggcccacga gcccttctgg
     1861 ctgtacgcat actccgtggt tcgggccacg gccttgtcgc cgtatgtcat gagccagtcg
     1921 tacgcgtcct tgtacaccgc gccttggttc ttcagattcc ggaactcctg aatctcggac
     1981 ttcagcagat cgaccttcgg gtgctgcagc cgtgcattgg ccagggcacc gttggcgcgc
     2041 cccccccggt caatggtgcg gcccgtattg acgccgctcg catccacact cgaatcgccc
     2101 agaatcgcgg ctccaccgcc gcacgccgtc tgcgtcgccc agtaaaaggc cacgcccaac
     2161 acacatagcc acgccgcgaa catctggacc gcgaacgtga tcagcaatcg gttcatccgc
     2221 cgaacccgtg tcggcaccgt gtggggccgc ttggccgacc gccggtcacg ccccatcctg
     2281 aggtcgaggt acgtcccgcc caaccaacgc ggcgtcgtca ccctcaaatc aacagcatgg
     2341 tgcccgccgg tcggctcccc tccggggggc cgaccccgtg cgcccgaccc gggg
//
LOCUS       DAOSPL010000718         2393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44279_length_2393_cov_3.129598, whole genome shotgun sequence.
ACCESSION   DAOSPL010000718 DAOSPL010000000
VERSION     DAOSPL010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44279_length_2393_cov_3.129598"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="PLQ54_12800"
     CDS             <1..1247
                     /locus_tag="PLQ54_12800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOS94189.1"
                     /translation="SPSALENPPVRHAVCTSVARRGIVNLITETPMLNRVLGMDDPLA
                     GRKGLTVLLLGTDQTNNLCDTVIVAFVNLETNRVGLLSVPRDSRVRTKGHGYCKLTEV
                     FPNAMMKSDRIEDGVAAVSNVIEESLGIPIDRYARVDIEGFVSLVDCLGGVELDVEKK
                     MHYRDRSQGLYIDLKPGLQKLDGKKAMQYVRFRNDERGDLGRVERQQKFLKAVSARLG
                     SLGRGDVIPLLGAAAKMRKYADTDLSLPELQALAKIVKDVDTGGVETRMASGTARMIG
                     GASYYVLDLPRVQQDILELQTQVAQSPTIAAVAKVAVLNGCATNGLAREAADKLTAKG
                     FEVTKVGNADSFRYVKTVVQYSPECRDAASRVAAALSVGEDALLETQELVDPEARVQV
                     VLGRDIMLVARGPSSSVGVSTH"
     gene            1286..1699
                     /gene="rsfS"
                     /locus_tag="PLQ54_12805"
     CDS             1286..1699
                     /gene="rsfS"
                     /locus_tag="PLQ54_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOS94190.1"
                     /translation="MDVPEHPGGPGRVPGRPTDSARLADLIFHVCDETKGQDVVVLDV
                     RLLTIVCDYFVIVHGLSLTHVQAIAEEIEDKLGEQGHTPHHKDREQDARWVVLDYGSV
                     VVHVFSEEARAFYRLEDLWAKAPIVRRQEAPETSA"
     gene            1808..>2393
                     /locus_tag="PLQ54_12810"
     CDS             1808..>2393
                     /locus_tag="PLQ54_12810"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS94191.1"
                     /translation="MSTQEGGPEARTWLRVQVLLKEAKTERAQGRYRGAVAKCQEALE
                     LAPDSLEVLETLGDIHVGAGHFSAAMESYRKVLQRDPSRPGVEEKLARASLSQLGFQR
                     QMDYGQRLLDRGAQADLKKASQIATSLSILFPGFGQLLNRHYWKALCMAAAHLCLILL
                     GFHVVMDAVRQGQAEHRVEIRSAPRSPYQRQPTMT"
BASE COUNT          428 a          711 c          830 g          424 t
ORIGIN      
        1 tctcgccgtc ggcgctcgag aacccaccgg tgcgccacgc ggtgtgcacg tcggttgccc
       61 gccgcgggat cgtcaacctg atcaccgaga caccgatgct caatcgggta ttgggtatgg
      121 acgatccgct ggcgggacgc aagggcctga cggtgcttct tctgggcacc gaccagacga
      181 acaatctctg cgacacggtg atcgtggcct ttgtgaacct cgagaccaat cgcgtcggct
      241 tgctctcggt ccctcgggac agccgcgtcc gcaccaaggg gcatgggtac tgcaagctga
      301 ctgaggtgtt tcccaacgcc atgatgaaga gcgatcgcat cgaggatggc gttgcggcgg
      361 tcagcaatgt catcgaggag tcgcttggca tcccgattga ccggtatgca cgggtggaca
      421 tcgagggttt cgtgtcgctg gtggactgct tgggcggcgt ggagctggac gtcgagaaga
      481 agatgcatta ccgggaccgg tcccagggcc tctacatcga cctcaaacca gggttgcaga
      541 agctggatgg caagaaagcc atgcagtatg tgcgcttcag gaacgacgaa cggggcgatt
      601 tggggcgtgt ggagcggcaa cagaagtttc tgaaggccgt cagtgcgcgt cttggttcgc
      661 tggggcgcgg cgacgtgatt ccgctgctcg gcgcggcggc gaagatgcgc aagtatgccg
      721 atacggatct gagcctcccg gagctacagg cgttggcgaa gatcgtcaaa gacgtcgaca
      781 cgggtggcgt cgagacgaga atggcctccg gtacggcgcg gatgatcggc ggggcgtcgt
      841 attatgtgct cgacctgccc agagtgcagc aggacatcct ggaactccag acccaggtag
      901 ctcagtcgcc gaccattgcc gctgtggcca aggtggccgt gctcaatggg tgcgcgacca
      961 acggactcgc tcgggaagcg gctgacaaac tgacggccaa aggattcgag gtcaccaaag
     1021 tcggtaatgc cgacagcttt cgctacgtca agacggtcgt tcagtactcg ccggagtgcc
     1081 gggatgccgc gagccgtgtg gcggcggcgc tctcggtggg cgaggacgcc ctgttggaga
     1141 cgcaggagct ggttgacccg gaggcgcgcg tgcaggtcgt cctcgggcgc gatatcatgc
     1201 ttgtggcccg gggtccgagt tcctccgttg gggtatctac ccactagggc cgatggacag
     1261 cccccatcga gttgacgcgg actgcatgga tgtccccgaa caccccggcg gccccgggcg
     1321 cgtgcccggt cgacccacgg actccgcccg actcgcggac ctgatcttcc acgtatgtga
     1381 tgagaccaag ggccaggatg tcgttgtcct ggacgtgcgt ctgctgacca tagtctgcga
     1441 ttactttgtg attgttcacg ggctctccct cacccatgtg caggccatcg cggaggagat
     1501 cgaggacaag ctgggggaac agggccacac gccgcatcac aaggaccgcg agcaagatgc
     1561 ccggtgggtg gtgctggact acggctcagt cgtggtccac gtgttctcgg aggaagcccg
     1621 cgccttctat cggctggagg acctgtgggc caaagctccc atcgtgcgcc gccaggaggc
     1681 tcctgagacc tcggcgtgag ctgtttggtg atcggggtca ccgacccggc ccatcggcac
     1741 tctcctgcat ccgaatgact gcgggcctgt gcccgcatgc gtgtgacgca acaggaggcc
     1801 cgggacgatg agcactcagg agggcggccc ggaggcgcgc acgtggcttc gcgtgcaggt
     1861 gctcctcaag gaagccaaga ccgagagagc gcaaggtcgg taccggggcg cggtagcgaa
     1921 gtgccaggag gcgctcgagt tggcccccga ctcgctggag gtgctggaga ccctcggcga
     1981 catacacgtg ggggccgggc acttcagtgc ggccatggag tcctaccgca aggtgctcca
     2041 gcgagatcca agccggccgg gcgtcgagga gaagctggca cgggcgagtc tgagtcagct
     2101 cgggttccaa cgccagatgg actacggcca gcgcctgctg gatcgcgggg ctcaggctga
     2161 cctgaagaag gcctcgcaga tcgccacctc actgtccatt cttttcccgg gcttcggcca
     2221 gctgctcaat cgccactatt ggaaggctct gtgcatggcc gcggcacacc tgtgcctgat
     2281 tctcttggga ttccatgtgg tgatggacgc cgtccgccag ggacaggcgg agcatcgcgt
     2341 ggagatccgc tcggcgccga ggtcgcccta tcagcggcag ccgaccatga cgg
//
LOCUS       DAOSPL010000719         2393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44280_length_2393_cov_3.092814, whole genome shotgun sequence.
ACCESSION   DAOSPL010000719 DAOSPL010000000
VERSION     DAOSPL010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44280_length_2393_cov_3.092814"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="PLQ54_12815"
     CDS             <1..1456
                     /locus_tag="PLQ54_12815"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOS94192.1"
                     /translation="GLMGRYIGLDLGTTTIIGLILDTDARQVVHAESARNDTRIAIRH
                     RRSLGRSEWHMVLMHQIVLELLRNLVHASGISQVDAIGVTGQMHGMVLLDGSNTPRSS
                     FIGWQDQRCQEPMPGGTTYIDEMLRRGGELFERSGCTPATGYMGSTLFWMRHNGKIPS
                     GTLACFAPDFVVSSLTDSRPVTDATNAAGSGLLDVANGCWNAELLEALGLDPAWLPEI
                     RPSLDLAGTLSAAAADATALPAGLPVAVASGDNQASFAGAVADYARSLLVNVGTGGQV
                     SVHVDEPVRGDGLDLRPFLRRGYLLVGASLCGGGSFRVLSDFIRAVGAEVFDLHDFDD
                     LDDRLTVLARSAPPGSEGLVCEPVFTGSRQDPLRRARWEGLSDTNFTPANLTRSLLEG
                     LAAQFGLFYDDMRRLGVEARTTLVGAGNGIRRNPVLAEILSSHFGATMRIASHTEEAA
                     VGAALGAAVTAGEFSDIEEAGRRFIRYDGISGGT"
     gene            1545..2336
                     /locus_tag="PLQ54_12820"
     CDS             1545..2336
                     /locus_tag="PLQ54_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOS94193.1"
                     /translation="MGMAAEVGLGVAGLLVVWVLCGLRAVYQYQKGVLFTFGRLTGEM
                     DPGLRLVLPIIQTWQRVDLRVRTIDVPGQECITKDNIQVHVNAVLYYAVQDACLSVTK
                     VQFPEYAVSQLAQTTMRNIVGEVELDQLLAERERLSERIRHIVDDFTDPWGIKIKKLE
                     LKDVVIPENMKRTIAKQAEAERERRSVIIRAEGEKMAAQDWAAAASELSAAPGGLHLR
                     TLQAINTISSDQSNTVVFCVPLEVLRALERLGGGLEQELGTRAGA"
BASE COUNT          365 a          741 c          856 g          431 t
ORIGIN      
        1 ggggctcatg gggcgctaca tcgggctgga ccttgggacg accacgatca tcggtctcat
       61 tctggatacg gacgcccgcc aggtggtcca cgcggagtcc gcgcgcaacg atacgcgcat
      121 cgccatccgc catcgccggt cattgggccg gtccgaatgg cacatggtgt tgatgcatca
      181 gatcgtgttg gagttgctgc ggaacctggt gcacgcatcg ggtatcagcc aggtggatgc
      241 cattggcgtg accgggcaga tgcacggtat ggtgctcctg gacgggtcca acacgccccg
      301 gtcgtctttc atcggttggc aggaccagcg ttgccaggag ccgatgcctg ggggcacgac
      361 ctacatcgac gagatgctcc gccgtggtgg ggagctcttc gagcgcagcg ggtgcacccc
      421 ggcgacaggg tatatgggct cgacgttgtt ctggatgcgc cacaacggga agatacccag
      481 tggaaccttg gcgtgtttcg cgccagattt cgtggtcagc tcgctgaccg attcgcggcc
      541 ggtcaccgat gccacgaacg ccgccggttc gggcttgctc gacgtggcga acggatgctg
      601 gaacgcggag cttctggagg cgctggggct ggatcccgcc tggctccccg agatccgccc
      661 ttcgcttgac ctggccggaa ccctctcggc ggccgcggcg gacgccacgg cactcccggc
      721 ggggcttccg gtcgcggtgg ccagtggtga taaccaggcg agtttcgcgg gagccgtggc
      781 cgactacgcg cggagcctgt tggtcaatgt gggcacaggg ggccaggtct cggtccatgt
      841 cgatgagccc gtccgtggcg acggtctgga cctccggcct ttcctgcgcc ggggctatct
      901 gctggtgggg gcgagcctct gcggaggagg ttcgttccgc gtcttgagcg acttcatccg
      961 ggcggtggga gctgaggtct tcgacctgca cgatttcgat gacctcgatg accggctcac
     1021 ggtgctggcg cgctctgcgc cccctggttc ggagggtctg gtatgtgagc ctgtcttcac
     1081 gggctcgcgg caagaccccc ttcggcgtgc ccggtgggag ggtttgagcg acaccaactt
     1141 cacgccggcc aacctgacgc gctccttgct cgaagggctc gcggctcaat tcgggctgtt
     1201 ctacgacgac atgcggcggc tgggagtgga ggcgaggacg acgctggtgg gcgccggcaa
     1261 tggcatccgc cggaacccgg tgctcgcgga gattctgtcg tcgcatttcg gcgcgacgat
     1321 gcgcatcgcg agccacaccg aggaagcggc ggtgggagcc gctcttggag ccgcggtcac
     1381 ggccggggag ttctccgaca tcgaggaggc cggtcggcgc ttcatccggt atgacgggat
     1441 ctccggcggc acgtaggtcc tcgccctgcg cgactcttgt gcgctccgag cgtcacgggc
     1501 tgtagagcgt ctattcgtcc ggaacgccgt gggagggatc gggaatgggc atggcagccg
     1561 aggtaggcct gggtgtggcg gggctgctgg ttgtgtgggt cttgtgtggg ctccgcgctg
     1621 tctaccagta tcagaagggc gtgctcttca ctttcggccg gctcactgga gagatggacc
     1681 cgggcctgcg gctcgtgctg cccatcatcc agacctggca gcgggtggat ctgcgcgtgc
     1741 gcaccatcga tgtacccggc caggagtgca tcaccaagga caacatccag gtgcacgtca
     1801 atgccgtgct ctactacgcg gtgcaggacg cgtgtctctc ggtcaccaag gtgcagttcc
     1861 ccgagtacgc ggtctcgcag ctcgcccaga cgaccatgcg gaacatcgtg ggcgaggtgg
     1921 agctggacca gctcctcgcc gagcgcgagc gcctctccga gcgcatccgg cacatcgtgg
     1981 atgatttcac cgacccctgg ggcatcaaga ttaagaaact cgaactcaag gatgttgtga
     2041 tccccgagaa catgaagcgc accatcgcga agcaggccga ggccgagcgc gagcgccggt
     2101 cggtcatcat ccgcgccgag ggcgagaaga tggccgccca ggactgggcc gcggcggcca
     2161 gtgagctctc ggccgcgccc ggtggcctgc atctccggac cctgcaggcc atcaatacga
     2221 tcagctcgga tcagagtaac acggtcgtgt tctgtgtccc gctcgaagtg ctccgggcgc
     2281 tggagcggct gggcggcggc ctcgagcagg agttgggcac tcgggcaggt gcctgaggtg
     2341 gcgcgcgcca ataggcaggc cgacacggcc ctggcggcct ccgccggggc cca
//
LOCUS       DAOSPL010000720         2392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44305_length_2392_cov_2.928113, whole genome shotgun sequence.
ACCESSION   DAOSPL010000720 DAOSPL010000000
VERSION     DAOSPL010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44305_length_2392_cov_2.928113"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PLQ54_12825"
     CDS             complement(<1..505)
                     /locus_tag="PLQ54_12825"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOS94194.1"
                     /translation="MRFVRMRFERVMVGLGLAALCLWGAALAQPGMDPAGLSGVRVSA
                     DTEYSAAHAAARVVDGTIQAPEGSWLSADWTPLPCTLTLELPATEVIRSIVLHQAVWS
                     GNMYHTQDFEIDASEDGRDWRRAGAGRLPDESGASVEVPLDGCRARWLRIRILTSYNP
                     QQTCGLAE"
     gene            647..823
                     /locus_tag="PLQ54_12830"
     CDS             647..823
                     /locus_tag="PLQ54_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94195.1"
                     /translation="MAKKDIADQCLCKAIECSAKLNDLRGLVRDAQYICKKCGRAAAK
                     KRNLCSPVKLYSEN"
     gene            881..1687
                     /locus_tag="PLQ54_12835"
     CDS             881..1687
                     /locus_tag="PLQ54_12835"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOS94196.1"
                     /translation="MQAVRQVLGRMVDLVFPPRCRVCETESACPLCPACVARLEYITG
                     PICHACGLPLDPGTHHGDRCAPCRDPHPLDRARSVCRYAGLTRKAIHELKYSQRTELA
                     PPLGDLMVRCLADPPEYGPAADPYYTPPPRIPLEGITAVVPVPLSREQQRRRGFNQSE
                     RLAIELGSRLGLPVFAWMLQRIRDTPPQVRLSARERARNVRGAFQAAGRDSVADQVVL
                     LVDDVLTTGATLNECARVLKRAGAADVYGITMARQTVLPTPEAAPASHVV"
     gene            1779..1997
                     /locus_tag="PLQ54_12840"
     CDS             1779..1997
                     /locus_tag="PLQ54_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94197.1"
                     /translation="MVVRYFGNLALGFLIGALTSELCGQRVTDYAAEGARKMGSAARQ
                     VVDAFRQGFAAPEPEPEAGPTRRPRRAK"
BASE COUNT          381 a          819 c          819 g          373 t
ORIGIN      
        1 cctcagccag gccgcacgtc tgctgcggat tgtaggaggt caggattcgg atgcgcagcc
       61 accgggcccg gcatccatcc agtggcacct cgacggaagc cccactctcg tcaggcaagc
      121 ggccggcgcc ggcacgccgc cagtcccggc cgtcctctga ggcatcgatc tcgaagtcct
      181 gggtgtggta catgttgccc gaccacacgg cctggtggag cacgatggac cggatcacct
      241 cggtggcggg tagctccaat gtcagtgtgc acggtagcgg tgtccagtcg gccgagagcc
      301 agctcccttc gggcgcctgg atggtgccgt ccaccaccct ggcggcggca tgtgccgcgc
      361 tgtactcggt gtccgcggag acgcgaaccc cactcagccc agccgggtcc attccgggtt
      421 gggccagggc ggcgccccac aggcacaggg cagccaggcc gagaccgacc atgacacgct
      481 cgaacctcat tcgcacaaac ctcattcgct cgaacctcca cagatcaccg aacgcatcgg
      541 ttcccggggc gcggtcccgg gaaccccggc agcgcgccca ccgatgtacc ttggtgagcc
      601 cgttgcctgt ccccatgccg gaaccgcaga ccatacggag gaccctatgg ccaagaagga
      661 tatcgccgat cagtgcctgt gcaaggccat cgagtgctcg gccaagctca atgacttgcg
      721 tggcctcgtc cgagatgccc agtacatctg caagaagtgt ggccgggcgg cggccaagaa
      781 gaggaacctg tgttcgccgg tgaaactata cagcgagaac tagccaagtg ggcgtgcgga
      841 gccgtgcggc ccggcgccca gcccgatgag atggttgccc atgcaggcgg tccggcaggt
      901 actcggccgg atggtggatc tggtgttccc gccccgctgt cgcgtctgcg agacggagag
      961 cgcctgcccc ctgtgtccgg cctgtgtggc ccgcctcgag tacatcaccg ggcccatctg
     1021 ccacgcgtgc ggcctgccgt tggaccctgg aacgcaccat ggggaccggt gtgccccctg
     1081 tcgagaccct cacccactcg atcgggcgcg gagtgtgtgc cgctacgcgg gcctcacgcg
     1141 caaggccatc cacgagctga agtactcgca acgcaccgag ctggctcctc cgttggggga
     1201 tctcatggtc cggtgcctgg ccgatccacc cgagtatggt ccggcggcgg acccctacta
     1261 cacaccgccg ccccgcatac ccctggaggg catcaccgcg gtggttcccg tgccgctctc
     1321 gcgcgagcag cagcgccgga ggggcttcaa ccagtccgag cggctggcca tcgaactggg
     1381 ctcccggctg gggcttccgg tgtttgcgtg gatgcttcag cgcattcgag acacgccgcc
     1441 gcaagtgcgg ctgtcggcca gggagcgagc gcgcaacgtg cggggcgcgt tccaggccgc
     1501 gggacgggat tcggtcgccg atcaggtcgt gctgctggtc gacgatgtcc tcacgaccgg
     1561 cgccacgctc aacgaatgtg cccgcgtgct caaacgcgcc ggcgcggccg atgtgtacgg
     1621 gatcaccatg gcgcgccaga cggttttgcc aaccccggaa gcggcgcccg catcccatgt
     1681 cgtgtaggat aagcgcaacc ggctcggcgg agggccgtct ttgagcccag tgggcacgaa
     1741 tgattcgcag gaccgtccca cagagggagg aagccgacat ggtagtccgc tacttcggga
     1801 atctggcgct ggggttcctc atcggtgcgc tcacatccga gctgtgcggt cagcgcgtga
     1861 cggactatgc ggccgagggc gcccgcaaga tgggctccgc ggcccgccag gtggtcgatg
     1921 ccttcaggca gggcttcgcg gcgccggaac cggaacccga ggccgggccc accaggaggc
     1981 cccgccgggc caagtagtcc ggcggacccg gcaccgcgag gtgtgtgagg gtccgctcac
     2041 aaccacgtgc gcgcgaacct ttgcgcgccg aatgcgtcta agacgccgag gggcaggtcc
     2101 atcggtcttg cctgacgtcc ggagcaggcc catgggcctg ccgcgcggcg gttatgccgc
     2161 tgggaaagtc gagtcctcgt gagcaggaaa cgcaactcca attccacctg gttgagcctc
     2221 atggaccggc gagtgctgtg ggcgcccgtg gccctggtgg ctctggccgt ggtcgtcggg
     2281 gccaccatgg cgccttgggc tggccgcgac cggcctgtct cgagcgccac caccgaggtc
     2341 actgcggccg gtacggatga acccgaggcg gctggaccgc gccgcctggt gg
//
LOCUS       DAOSPL010000721         2386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44464_length_2386_cov_3.846418, whole genome shotgun sequence.
ACCESSION   DAOSPL010000721 DAOSPL010000000
VERSION     DAOSPL010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44464_length_2386_cov_3.846418"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLQ54_12845"
     CDS             complement(<1..336)
                     /locus_tag="PLQ54_12845"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOS94198.1"
                     /translation="MAVSGKRTASNALAVRSANLLAVLACLRDGRAMSTPDLGEALQL
                     HLSTVTRLMAVLRRAGLVEPERGANSSPAVGRPPTHWRMVRDAGYALGLALHPGRVSG
                     VVTDLTGHRL"
     gene            complement(374..568)
                     /locus_tag="PLQ54_12850"
     CDS             complement(374..568)
                     /locus_tag="PLQ54_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94199.1"
                     /translation="MTRSAKVIMSALLATAFAVMLLACGCGKKGGEPTREPTTADYAK
                     KAEALKASGVQKPWMTKKGQ"
     gene            complement(673..1113)
                     /locus_tag="PLQ54_12855"
     CDS             complement(673..1113)
                     /locus_tag="PLQ54_12855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94200.1"
                     /translation="MRTTESTPASLWWYDLVQPYVKNQQILACPSQPKSLNNGTLGYG
                     INCCMLCPHWCAPSSRPYLGEIPRPADILFIADSAIWLACSWEVAWANVCEVSCTPSV
                     RIDSNTRHVGGSNTLFCDGHAKWFNAQTIGNQAWMDAHIINYKQ"
     gene            complement(1129..1308)
                     /locus_tag="PLQ54_12860"
     CDS             complement(1129..1308)
                     /locus_tag="PLQ54_12860"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS94201.1"
                     /translation="MRKGFTLIELLVVIAIIAILAAILFPVFAKAREKARQTSCLNNV
                     KQIGTGMLMYAQDYD"
     gene            1503..>2386
                     /locus_tag="PLQ54_12865"
     CDS             1503..>2386
                     /locus_tag="PLQ54_12865"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HOS94202.1"
                     /translation="MRIPRPEYPRPDLRRGREEGRDWLNLNGPWLFAFDPGDEGLGAE
                     WQHRPNEAFPTTLLVPFPWESPAAWGQDRERRDWNEEFLVREAYLDPGGVGMDNYRTA
                     ERHTIGWYRRTVTIPESWRGRPVFLNFGAVDHHATVFVNGIRLGEHEGGYTPFGFDIT
                     GALGETGPAVITVRAQDPQDHRDQPAGKQIHWYQRTSGIWQTVYLEPRGLCYVEQVHA
                     RPDVDAEAVEFRIWLGGDEIPGATVEVTVVDPDEAEAGRVSAAASVGEVLRVGLGLPD
                     VTLWDIDAPALYRAQVCV"
BASE COUNT          381 a          745 c          860 g          400 t
ORIGIN      
        1 caggcggtgc ccggtcaggt cggtgacgac gccgctcact cgccccggat ggagcgccag
       61 gcccagcgcg tagcccgcat cgcggaccat tcgccagtgc gtgggcgggc ggccgacagc
      121 gggcgacgag ttcgcgccgc gctcgggctc gaccagcccc gcgcggcgca ggacggccat
      181 caggcgggtg acggtggaga ggtggagctg gagagcctca cccaggtccg gggtgctcat
      241 ggcgcggccg tcgcgcaggc aggccaggac cgccaggagg ttggcggacc ggaccgcgag
      301 cgcgttgctg gctgtgcgtt tgcctgagac ggccatgggt gaggctctcc gtgatgggtt
      361 gctggacggg cggctactga cccttcttgg tcatccacgg cttctgcaca cccgatgcct
      421 tgagggcctc agccttcttg gcatagtcgg cagtggtggg ttcgcgggtg ggctcgccgc
      481 ccttcttgcc gcagccacac gccagcagca tcaccgcgaa tgccgtagca agtagcgcac
      541 tcatgataac cttcgccgag cgagtcatca gctgtcctcc cgaggggcgt tcccagtgtg
      601 cagcagccgc gtggggaggc ggcggagtcg gtgcgctcgc cccccccggc tgcccgatgg
      661 gcttcggtcg agctactgct tgtagttgat gatgtgggca tccatccagg cctggttgcc
      721 gatagtctgg gcgttgaacc acttggcgtg cccatcgcag aacagcgtgt tgctgccccc
      781 cacgtggcgg gtgttcgagt cgatgcgcac cgacggggta caggagacct cacacacgtt
      841 ggcccaggcc acctcccacg agcaggccag ccagatggcg gagtcggcaa tgaagagaat
      901 gtcggccggc cgggggatct cgccgaggta cggacgcgag gaaggcgcgc accagtgagg
      961 gcacagcatg cagcagttga tgccatagcc cagtgtgccg ttgttcaggc tcttgggctg
     1021 ggaggggcag gcgaggatct gctggttctt cacgtaaggc tggaccagat cataccacca
     1081 gagcgatgcc ggcgtgctct cagtcgtgcg cacaccgaca gggagaccct agtcgtagtc
     1141 ctgggcgtac atgagcatgc cggtaccgat ttgcttgacg ttgttgaggc agctggtctg
     1201 acgggccttc tcgcgagcct tcgcgaagac gggaaacagg atcgcggcaa ggatcgcgat
     1261 aatggcaatg acgacgagca gttcgatcag cgtaaagccc ttccgcatgt tcggtccccc
     1321 cacgcagacg atacacgcac tggcgcacat tacttgcgtg ctgcaagcaa gtatatggcg
     1381 gcccttacgc cgagtcaaca gggagttggc gcagagtctt gcggcgtcag gggccgcttc
     1441 gcgccgcggt gttcagccgc gggaccccgt gctagcatga ggctgacggg ggctgatgct
     1501 ggatgcgcat tccacgaccg gagtacccga ggcccgatct gcgccgcggc cgcgaggagg
     1561 ggcgtgactg gctcaacctg aacggtccct ggctgtttgc cttcgacccg ggcgacgagg
     1621 ggttgggcgc cgagtggcag catcggccca acgaggcgtt cccgacgacg ctgctggttc
     1681 cgtttccctg ggagagtccg gcggcatggg gtcaggaccg tgagcggcgc gactggaacg
     1741 aggagttcct cgtccgcgag gcctacctcg acccgggcgg cgtcggcatg gacaactacc
     1801 gcacggccga gaggcacacc atcggttggt accgtcgcac ggtgacgatc ccggagagct
     1861 ggcgcggccg ccccgtgttc ctgaacttcg gcgccgtcga ccatcacgcg acggtgttcg
     1921 tcaacggcat ccggctgggc gagcacgagg gcggctacac gccctttggc ttcgacatca
     1981 ccggcgcgct gggggagacc ggcccggccg tcatcaccgt ccgcgctcag gatccccagg
     2041 atcaccgcga ccagcccgcg ggcaagcaga tccactggta ccaacggacc agtggcattt
     2101 ggcagacggt ctacctcgag ccgcgcgggt tgtgctatgt ggagcaggtt cacgcgcggc
     2161 cggatgtcga cgccgaagcg gtcgagttcc gcatctggct gggtggcgac gagataccgg
     2221 gggccacggt ggaggtcacc gtggtcgatc ccgatgaggc tgaggcgggt cgggtgtcgg
     2281 cggcggcctc cgtgggagag gtgcttcgcg tcgggctcgg actgcccgac gtgaccctgt
     2341 gggatatcga tgccccggcg ctctatcggg cgcaggtctg cgtgat
//
LOCUS       DAOSPL010000722         2385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44498_length_2385_cov_2.738197, whole genome shotgun sequence.
ACCESSION   DAOSPL010000722 DAOSPL010000000
VERSION     DAOSPL010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44498_length_2385_cov_2.738197"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="PLQ54_12870"
     CDS             complement(<1..1228)
                     /locus_tag="PLQ54_12870"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS94203.1"
                     /translation="MHLKSLHITGFKTFADPTTFEFPMGVVGIVGPNGSGKSNIADAI
                     LWALGEQSLKSLRGSVLTDVIFAGSQARKPTNMAEVVLTIDNADGQIGLDFTEIAVER
                     RAFRTGDSEYCINKAKCRLRDIHDLFLDTGVGRGAYSIIGQNEVDRVLSARPEDRRAL
                     FEEAAGVSRYRTRRLEAMSKLESTQANLNRINDILREVEEQIGPMAGQAEAAREHRVL
                     AAQLREISLGLYVTDYDRRVKENGKLEHEIFVLGADIERLSGQVATLETEEQGLRLAL
                     GEVEEAIDALRRDNSQVTAALSAARADQRVRQERLRAVASDRERLTAEQTRLAQRASE
                     MPAELSALEAAASALRDRMAELEASRTELEGGLRYATNRFQQAAEESQAKARAAAEAA
                     AQRAEGQRVLASLRAAS"
     gene            1343..>2385
                     /locus_tag="PLQ54_12875"
     CDS             1343..>2385
                     /locus_tag="PLQ54_12875"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOS94204.1"
                     /translation="METVVRDRLRGALVGLAVGDALGASVEFCAPGTFEPITGYRGGG
                     PHGLDPGEWTDDTSMALALADSIAHGWDLNDQARRYVDWQQTGRYSVNGRCFDIGVQT
                     SRALNAFRRSGDAHTSGGSSEDSSGNGSIMRLAPVPIAFISLYPDRVDELALRAEESS
                     LPTHGSPICRSSCRYLGVVLAALASGEDREQVLAPDWSVLDCLGPLHPLVDAIARGSF
                     REKQPPAIRGTGFVVDCLEAALWAFHAGEDFADAVLRAVNLGDDADTTGAVCGQLAGA
                     YWGEAGIPDAWRAGLARRDMIEAALAGLGLLCGPRAKGIVDAGGVRPLALAVAGVAAA
                     AAVAWVAWRGVTRR"
BASE COUNT          331 a          836 c          812 g          406 t
ORIGIN      
        1 ccgaggcagc tcgcagcgag gccagcacgc gttggccctc ggcccgttga gccgcggcct
       61 cggcggcggc cctggccttg gcctggctct cctcggccgc ctgctgaaag cggttcgttg
      121 catagcgcaa cccaccctca agctcggtcc ggctcgcctc gagctccgcc attcgatcgc
      181 gcagtgccga tgcggctgct tcgagggcgc tcagctcggc gggcatctcg gacgcgcgtt
      241 gtgccagacg agtctgctcg gcggtgaggc gttcgcgatc ggacgccacg gcgcggagac
      301 gctcctggcg cacccgctgg tcggcccgag ccgcagacag tgctgcggtg acctggctgt
      361 tatcgcgccg cagggcgtcg attgcctctt cgacctcgcc gagcgccaac cgcagcccct
      421 gctcctcggt ctcgagcgtg gcgacctggc cgctaagccg ctcgatgtca gcccccagca
      481 cgaagatctc gtgttcgagc ttcccgttct ccttgacgcg gcggtcgtag tcggttacgt
      541 acaatcctag tgatatttca cgaagctggg cggccaggac acggtgctcg cgggcggcct
      601 cggcctggcc cgccatgggg ccgatctgct cctctacctc gcgcaggatg tcgttgatcc
      661 ggttgaggtt cgcctgcgtg ctctcgagct tcgacatggc ctcgaggcga cgagtgcggt
      721 agcgcgagac gccggcggcc tcctcaaaca gcgcgcgtcg gtcctcgggg cgcgcgctca
      781 gcacccggtc gacctcgttc tggccgatga tcgagtaggc gcctcggccg actcccgtgt
      841 ccaggaagag gtcgtggatg tcccgcaggc ggcacttggc cttgttgatg cagtactcgc
      901 tgtctcccgt acgaaaggct cggcgctcga cggcgatctc ggtgaagtcc agcccgatct
      961 ggccgtcggc attgtcgatc gtcaggacga cctcggccat gttggtgggc ttgcgcgcct
     1021 gggagccggc gaagatcaca tcggtcagca cactgccccg gagactcttg agtgattgct
     1081 cgcccaatgc ccacaggatc gcatcggcga tgttgctctt cccgctgccg ttcgggccga
     1141 caatccccac gactcccatg gggaactcga aggtggtggg atccgcgaag gtcttgaagc
     1201 cggtgatatg gagtgacttg aggtgcaaca aaggcctcca gggctcatgg ccgaaacgga
     1261 gtgcagtgta gcgggtacac cccccaagct caagggcacc cgccacaccg gagcccgtgt
     1321 gaactcaagg agtgccgcag gcatggagac cgtcgtccgc gaccgcctca gaggggctct
     1381 tgtcggcctc gccgtcggcg atgccctcgg agcctccgtc gagttctgcg cgccgggcac
     1441 cttcgagccg atcaccgggt accgcggcgg cggaccccac ggactcgatc cgggcgagtg
     1501 gaccgacgac acctcgatgg ccctggcgct ggccgatagc atcgctcacg gttgggacct
     1561 caacgaccaa gcccgccggt atgtcgactg gcagcagacg ggccgctact cggtgaacgg
     1621 ccggtgcttc gacatcggcg tccagacgtc gagggctctg aatgccttcc gcagatcggg
     1681 ggacgcgcac acgtccggcg gctcctcgga ggactccagc ggcaacggct cgatcatgcg
     1741 actcgcgcct gtgcccatcg cattcatctc gctctaccct gaccgcgtcg acgaactggc
     1801 cctgcgcgcc gaggagtcga gtctgcccac acacgggtca ccgatctgcc gctcgtcgtg
     1861 ccgctacctc ggcgtcgtcc tggccgcgct ggcgtcgggg gaggaccgcg agcaagtgct
     1921 ggcacctgac tggagcgtgc tggactgcct ggggcccctc catcccctcg tcgacgccat
     1981 cgcccgcggc agcttccgtg agaagcagcc ccctgccatc cggggcactg ggttcgttgt
     2041 cgactgcctg gaagccgccc tgtgggcctt ccatgccggt gaagacttcg ccgatgccgt
     2101 cctccgcgcc gtcaacctgg gcgatgatgc cgacaccacg ggcgccgtct gtggccagct
     2161 ggctggggcc tactggggag aggcaggcat cccagacgcc tggcgcgccg gcctggctcg
     2221 gcgcgacatg atcgaggcgg ccctcgccgg actcgggctc ctatgcgggc cccgggccaa
     2281 ggggatcgtc gacgccgggg gcgtgcggcc gctcgccctc gccgtcgccg gggtggctgc
     2341 cgcggccgcg gtggcctggg tcgcctggcg gggggtcacc cggcg
//
LOCUS       DAOSPL010000723         2384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44523_length_2384_cov_3.601546, whole genome shotgun sequence.
ACCESSION   DAOSPL010000723 DAOSPL010000000
VERSION     DAOSPL010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44523_length_2384_cov_3.601546"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2384
                     /locus_tag="PLQ54_12880"
     CDS             <1..>2384
                     /locus_tag="PLQ54_12880"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOS94205.1"
                     /translation="FFARGEEHPYLSKLSKDELKTILGGKGYGVYRMICQLGLKCPAM
                     FNLPTYHANNLAEGKLTPELRDEVCRNIALLEKATGKKFGSPENPLLVSARSGAKFSM
                     PGMMDTILNIGLNDEIVEALVKAQPANARFWYDAYRRLLEMIGDVVYGIAEYDAGEET
                     KDRFEEAMEQVKHAAGVTNDAELTADHLKTLCGKFKEIYKSQGQEFPSDPVEQVCLAA
                     EAVFRSWNTPRAIFTRKEDGIPDDLGTAVNIQEMVFGNKTPLSGTGVFFTRDKSTGHK
                     TPGKLDGTVLFQAQGEDVVAGRRNSEPLEVLLESENEHLRAIYNEIKETGDLLERAER
                     NMQDTEFTIEENAEGVPILYWLQIRDGKRSALAESVIAVNLAKEGIITEAEAVLMVNP
                     KRFEEQLYPRIASSAKQSARLLGKGTAASPGPAAGAITFTKDQALEDSKTKDVVLCTV
                     MTNQNDVPGMKAAVGILTSTGTKVSHAAIMATAWGVPAVVGASCVSFGEDSEGRFLEI
                     DGTKLRRGDVITIDGASGEIFLGALPSEKPSDLSPEAEQIIEWAKKLKSLQIRANAER
                     AEAQISADRGAEGIGLFRTEHMFLGERLGDIQTVLFGADEAAKVAALDRIAQFSKSDF
                     KDALRIMNGYGVTIRLLDAPLHEFFPEDSHLEREENPMLGFRSVRMCYLNPEIARMQA
                     WAILTAAAELQKEGLDPHPEIELPLVIIPEEVAYFKKLVAEVAVKVHEETGVWVHYGL
                     GIMVETPAAALQGTEMVKALEAPVCGVPAEAQSAKGFASFGTNDLTQTTLAISR"
BASE COUNT          471 a          755 c          760 g          398 t
ORIGIN      
        1 ttctttgcac ggggcgagga acacccctat ctgagcaagc tctctaagga tgaactgaag
       61 accatcctcg gcggcaaagg ctatggcgtg taccgtatga tctgccagct ggggctgaag
      121 tgccccgcca tgttcaacct gccgacgtac catgccaaca acctggccga ggggaagctc
      181 acccctgagc tgcgtgacga ggtgtgccgg aacatcgcgc tcctcgagaa ggctacgggc
      241 aagaagttcg ggagccccga gaacccgctg ttggtctccg cccgttcggg cgccaagttc
      301 tcgatgccgg gcatgatgga caccatcctg aacatcggcc tcaatgacga gatcgtcgag
      361 gcgctcgtga aagcccagcc ggccaacgct cggttctggt atgacgcata tcgacgtctt
      421 ctcgagatga tcggtgacgt ggtctacggc atcgcggagt acgacgcggg cgaggagacc
      481 aaggaccgct tcgaggaggc catggagcag gtcaagcatg ccgcgggggt gaccaacgac
      541 gcggagctga cggcggacca cctcaagacc ctgtgcggca agttcaagga gatctacaag
      601 tcccagggcc aggaattccc ctctgacccc gtggagcagg tctgtctggc cgccgaggcc
      661 gtcttccggt cgtggaacac cccgcgcgcc atcttcaccc gcaaggaaga cggtattccc
      721 gacgatctgg gcaccgccgt caacatccag gagatggtgt tcggcaacaa gaccccgctg
      781 tcgggcacgg gcgtcttctt cacgcgcgac aagtccacgg gccacaagac cccgggcaaa
      841 ctggacggca cggtgctctt ccaggcacag ggcgaggacg tggtcgccgg ccggcgcaac
      901 tccgagccgc tggaggtgct cctcgagtcg gagaacgagc acctgcgcgc tatctacaac
      961 gagatcaagg agacgggcga cctgctcgag cgcgcggagc gcaacatgca ggacaccgag
     1021 ttcaccatcg aggagaacgc cgaaggcgtg cccatcctgt actggctgca gatccgtgac
     1081 ggcaagcgtt cggccctggc cgagtccgtg atcgccgtca acctggccaa ggagggcatc
     1141 atcaccgagg ctgaagcggt cctgatggtg aaccccaagc gctttgagga gcagctctac
     1201 ccacgaatcg cgtccagcgc aaagcagtcg gcgcgcctcc tcggcaaggg cacggcggcc
     1261 tctccgggcc cggcagccgg tgcgatcacc ttcaccaagg accaggcact tgaggactcc
     1321 aaaaccaagg acgttgtcct ctgcaccgtg atgaccaacc agaacgatgt ccccggcatg
     1381 aaggccgcag tcggcatcct gacctccacc ggcaccaaag tctcgcatgc cgccatcatg
     1441 gccacggcct ggggcgtgcc tgcggtcgtc ggggcgtcgt gtgtatcatt tggtgaggac
     1501 agtgaggggc gcttccttga gatcgatggc accaagcttc gccgcggcga tgtcatcacc
     1561 atcgatggcg cgtcgggcga gatcttcctg ggtgcgctgc ccagcgagaa accgagcgat
     1621 ctgagccccg aggccgaaca gatcatcgag tgggccaaga agctcaaatc actccagatc
     1681 cgcgccaatg ccgagcgcgc cgaggctcag atctccgccg accgcggagc cgagggcatc
     1741 ggtttgttcc gcaccgagca catgttcctt ggagagcgcc tcggggacat tcagaccgtg
     1801 ctgtttggtg ccgatgaggc agccaaggtg gcagccctcg accgcatcgc ccagttctcc
     1861 aagtccgact tcaaagatgc tttgcgtatc atgaatggtt acggcgtgac catccgcttg
     1921 ctggatgctc ccttgcacga gttcttcccc gaggattcgc acctcgagcg cgaggagaac
     1981 ccgatgctgg gcttccggtc ggtgcgcatg tgctacctga accccgagat tgccaggatg
     2041 caggcgtggg ccattctcac cgccgcggcc gagcttcaga aggagggact ggacccccat
     2101 ccggagatcg agctgcctct ggtcatcatc ccggaggagg tggcctactt caagaaactc
     2161 gtggccgagg tggcagtgaa ggttcacgag gagacggggg tttgggtgca ctacggcctg
     2221 ggcattatgg tcgagactcc ggccgccgct ctgcagggca ccgagatggt caaggctctg
     2281 gaggcgcccg tgtgtggtgt ccccgccgag gcgcagtcgg ccaagggctt tgcgtccttc
     2341 gggaccaacg acctgaccca gaccacgctg gccatcagcc gtga
//
LOCUS       DAOSPL010000724         2381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44620_length_2381_cov_2.879622, whole genome shotgun sequence.
ACCESSION   DAOSPL010000724 DAOSPL010000000
VERSION     DAOSPL010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44620_length_2381_cov_2.879622"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PLQ54_12885"
     CDS             complement(<1..669)
                     /locus_tag="PLQ54_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOS94206.1"
                     /translation="MDTISPLTSEITLESEIKDSYMTYAMSVIFARALPDARDGLKPV
                     QRRILYTMHEMGLAPNRQHVKCAKVTGEVMGKYHPHGDQVIYPALVRLAQDFNQRYLM
                     VDGQGNFGSVDGDPPAAQRYTECRLARAAVPMMEDIDEDTVDFQPNYDDREQEPSVLP
                     GRFPNLLCNGGTGIAVGMATNIPPHNVNEICDGIIELIRRPEMTVQDLMQIVHGPDFP
                     TAGFI"
     gene            complement(787..>2381)
                     /locus_tag="PLQ54_12890"
     CDS             complement(787..>2381)
                     /locus_tag="PLQ54_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOS94207.1"
                     /translation="YERGVPKTKLEVVGKAKGTGTMIRFKPDPEIFEVTEFDYEVIAR
                     RLRELAYLTGGATISLADERTGKRESFSYKGGIAEYVKTLNRNKGVLHRTPVLIAAER
                     DSIGIEVAIQYNDGYAEQVLSFANFINTREGGTHVSGFRTALTRTVNQYGRKLGVLKE
                     KDPNLSGDDVREGLCAVVSVKLLNPQFEGQTKAKLGNSEVEGIVNSVVGEKLSEWFEE
                     HPAEAKRIISQAQVAQRARDAARKASDLIKRKSALESGSMPGKLWDCSSRKREECEVF
                     LVEGDSAAGSAKSARDARFQAILPLGGKPLTVEKARIDKVLSHEVLGTIISALGTGIT
                     AHSNGNGKAEAQEELGEELQDEGDGEGEAQPGTAGEADGPDNGTMDLSKLRYERVIIM
                     ADADSDGLHIRTLLLNFFFRYMQPLITHGHVYIARPPLFRVKHGREIDYAWSEEELNR
                     LLERTSPRGRQVSRFKGLGEMDAEDLALTTMDIATRRISRVTIDDCVMADDLFVTLLG
                     EKVEPRREFIATHAKEVRNLDF"
BASE COUNT          416 a          796 c          697 g          472 t
ORIGIN      
        1 gatgaagccc gcggtgggga agtcgggccc gtggacgatc tgcatcaggt cctggacggt
       61 catctccgga cgacggatca gctcgatgat gccgtcacag atctcgttca cgttgtgcgg
      121 cgggatgttc gtcgccatgc ccaccgcgat accggtgccg ccattgcaga gcagattcgg
      181 gaagcgcccc ggcagcaccg agggctcctg ttcgcggtca tcgtagttgg gctggaagtc
      241 gacggtatcc tcgtcgatgt cttccatcat cggcactgcg gcccgggcca gccggcactc
      301 ggtgtaccgc tgagcggccg gcggatcacc atcgacgctg ccgaagttcc cctgcccatc
      361 gaccataagg taccgctggt tgaagtcctg agcgagacgc acgagggcag ggtagatcac
      421 ctgatcaccg tgcgggtgat acttacccat cacctctcca gtgaccttgg cgcacttgac
      481 atgctgccga ttgggtgcca gacccatctc atgcatggtg tacaggatcc gccgctgcac
      541 gggcttgagc ccatcgcgcg catccggcag ggcccgtgcg aagatcacgc tcatggcgta
      601 cgtcatgtat gagtccttga tctccgactc gagcgtgatc tcggaggtca acgggctgat
      661 ggtgtccatg gtcggagctc ctcaacatac gaacgaatca cgaagaggca acagcagagc
      721 agtggccgga cggagactcc aaacacgcgc cgccccggcc gcagctatgg cccgtatcga
      781 cgcgggctag aagtcgagat tgcggacttc cttggcgtgg gtcgcaatga actcccgccg
      841 tggctcgacc ttctcgccca acagcgtcac aaacaggtcg tcagccatta cacagtcgtc
      901 gatggtcacc cgcgagatcc gccgggtcgc gatgtccatc gtcgtcagag ccaggtcctc
      961 ggcatccatc tcgcccagac ccttgaagcg cgatacctgc cgcccgcgcg gcgacgtccg
     1021 ctccaagagg cggttcagct cttcctcaga ccaggcgtag tcaatctcgc ggccatgctt
     1081 gacccgaaac aagggcgggc gcgcgatgta gacatgcccg tgggtgatca acggctgcat
     1141 gtaccggaag aagaagttca ggagcagagt ccgaatgtga aggccgtccg agtcggcgtc
     1201 ggccatgatg atgacccgtt catagcgcag tttcgacaga tccattgtcc cgttgtcggg
     1261 cccatcagct tccccggcgg tcccgggttg cgcctcgccc tcgccgtcgc cttcgtcctg
     1321 cagctcttcg ccgagctcct cctgagcctc ggccttgcca ttcccgttgg agtgggcggt
     1381 gatcccagtc cccagagccg agatgatggt ccccaacacc tcgtggctga gcactttgtc
     1441 gatgcgcgcc ttctccacgg tcaggggctt gccacccagg ggaaggatcg cctgaaaacg
     1501 cgcgtcgcga gcgctcttgg cgctccccgc cgcgctatca ccctcgacga ggaagacctc
     1561 acactcctca cgcttgcgcg acgagcagtc ccagagcttc ccgggcatcg aaccactctc
     1621 gagcgccgac ttgcgcttga tgaggtcgct ggcctttcgg gccgcatcgc gcgcccgctg
     1681 cgccacctgc gcctggctga taatgcgctt ggcctcggcc ggatgctcct cgaaccactc
     1741 cgagagcttc tcacccacca ccgagttgac gataccctca acctcgctgt tacccagctt
     1801 tgccttggtc tgaccctcaa actgcgggtt gagcagcttc acgctgacca ccgcgcacag
     1861 gccctcgcgg acatcgtcgc cggagaggtt tggatccttc tcctttagca ccccaagctt
     1921 gcggccatac tggttgacgg ttcgcgtgag ggccgtgcgg aagccgctga cgtgcgtgcc
     1981 gccttctcgt gtattgatga agttcgcaaa agagagtacc tgttcggcat atccgtcgtt
     2041 atactggata gccacctcga taccaatgct gtcgcgctcg gctgcgatga gcactggcgt
     2101 ccgatgcagc actcccttat tgcgattcag ggttttcaca tactcagcga tgccgccctt
     2161 gtaggagaac gactcgcgct tgcccgtgcg ctcgtctgcc aggctgatcg tggccccgcc
     2221 ggtcagatag gcaagctccc tcagccggcg cgcgatcacc tcgtagtcaa actcggtgac
     2281 ctcgaagatc tcggggtcgg gcttgaaccg gatcatggtt ccggtgccct ttgccttccc
     2341 caccacttca agcttagtct tcggcacgcc ccgctcatac c
//
LOCUS       DAOSPL010000725         2381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44628_length_2381_cov_2.369304, whole genome shotgun sequence.
ACCESSION   DAOSPL010000725 DAOSPL010000000
VERSION     DAOSPL010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44628_length_2381_cov_2.369304"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PLQ54_12895"
     CDS             <1..557
                     /locus_tag="PLQ54_12895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOS94208.1"
                     /translation="TGIVDMVRQFSRERQAGMTVVPVTEVLDDVIALQRFDEAVRRTQ
                     IETEYACHPEVDGNADRLRQVFLNLIRNAGQALQGRPDGRIIVRVRQEDQQVIIEVAD
                     NGPGVPAEIQERIWEPFYTTRGAEGTGLGLDICRQIVAQHRGTIGLESEPGRGATFTV
                     RLPASGSRPPEETEDANSDGENDA"
     gene            577..1824
                     /gene="folP"
                     /locus_tag="PLQ54_12900"
     CDS             577..1824
                     /gene="folP"
                     /locus_tag="PLQ54_12900"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOS94209.1"
                     /translation="MHAVRPVVFHTLDEARQAMAHLGADGRRIELMAAKSVHRCLYIP
                     DLPVRAAVILKQEMLAKGGEAVLPWEALCLRGERTAVLLCGTLRQYANVCLTLRDQPF
                     GLPELAVRIEACLHRGAGDGAPRALRIGNAAWPLGRRTYVMGIVNMTPDSFSGDGLAP
                     DEPAVARAVARAQQMCADGADIIDVGGESTRPGAEPVDADEQTRRVVPLIRELAALGI
                     PVSVDTRSARVAEAAIAVGAVLVNDVTALTGDPDMRRVCGQAGVAVCLMHMRGDPVSM
                     QANPTYETDVVLEVAQFLERQVEVACAAGVDPGAILVDPGIGFGKTVDHNLELLQRLD
                     ELRTLTAQPVLVGTSRKSVIGQVLGLPVDDRLEGTAATVACAIARGADMVRVHDVREM
                     TRVCRMTDAVLRREVRIASSSRG"
     gene            1821..2159
                     /gene="folB"
                     /locus_tag="PLQ54_12905"
     CDS             1821..2159
                     /gene="folB"
                     /locus_tag="PLQ54_12905"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018759447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOS94210.1"
                     /translation="MIFRGHHGVSEAERSLVQRFRVDVALELDLQPAARADDLSRACD
                     YSRVFETVQHLVEKRTFQLLETLAERIAEAILDGFPAVTALTVGVFKPDPPMHGTFER
                     VGVEICRTRP"
     gene            2156..>2381
                     /locus_tag="PLQ54_12910"
     CDS             2156..>2381
                     /locus_tag="PLQ54_12910"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017588278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOS94211.1"
                     /translation="MTRAYLGLGSNLGDRLEALRRAVRELATTPGIVVRHVSSCYETA
                     PVGPVNQGDFLNAALAIAWVEKAAAAGDDVV"
BASE COUNT          363 a          864 c          810 g          344 t
ORIGIN      
        1 ccaccggcat cgtggacatg gttcgccagt tcagccggga gcgtcaggcc ggcatgacgg
       61 tggtcccggt caccgaggta ctagacgacg tcatcgcgct ccagcgcttc gacgaggccg
      121 tccggcgcac gcagatcgag accgagtacg cgtgccaccc ggaggtcgac ggcaatgccg
      181 accggctacg gcaggtgttc cttaacctca tccgcaacgc cggccaggcg ctccagggcc
      241 ggcccgacgg ccgaatcatc gtccgggtgc ggcaggagga ccagcaggtc atcatcgagg
      301 tggccgacaa cggcccgggt gtcccggccg agatccagga gcggatctgg gagcccttct
      361 acaccacccg gggcgccgag ggcaccggcc ttgggctcga catctgccgc cagatcgtcg
      421 cccagcaccg gggcaccatc ggactcgagt cggagcccgg ccgcggcgcc accttcacgg
      481 tgcgactgcc cgcctcgggc tcgcggccgc ccgaggaaac cgaggacgcc aacagcgacg
      541 gcgagaacga cgcgtagcca cgcggccgga ggagccatgc acgcagtccg cccagtggtc
      601 tttcacacgc tcgacgaggc acgtcaggcc atggcccatc tcggcgccga cggccgccgg
      661 atcgaactga tggccgccaa gtccgttcac cgctgcctct acatccccga cctccccgtc
      721 cgcgccgcgg tcatcctcaa gcaggagatg ctggccaagg gcggcgaggc cgtgctcccc
      781 tgggaagccc tgtgcctccg gggcgagagg acggccgtcc tcctctgtgg cacgctccgc
      841 cagtatgcca acgtctgcct gacactgcgc gaccagccct tcggcttgcc cgagctggcg
      901 gtccggatcg aggcgtgcct gcaccggggc gccggtgatg gggccccacg ggcactgcgg
      961 atcggcaacg ccgcatggcc cctcggtcgg cgcacctatg tcatgggcat cgtcaacatg
     1021 accccggact ccttctcggg cgacggcctc gcgccggacg aaccggccgt cgctcgcgct
     1081 gtggcccggg cccagcagat gtgcgccgac ggcgcggaca tcatcgacgt gggcggcgaa
     1141 tcgacccgcc ccggcgccga gcctgtcgat gccgacgagc agacacggcg cgtggtcccc
     1201 ctgatccgcg aactggccgc tctcggcatt ccggtgtcgg tcgacacccg cagcgcccgg
     1261 gtggccgagg ccgccatcgc cgtgggtgcg gttctggtca acgacgtcac cgcgctcacc
     1321 ggcgaccccg acatgcgtcg cgtctgcggc caggccggtg tcgcggtctg cctcatgcac
     1381 atgcggggcg acccggtgag catgcaggcg aatcccacct acgaaacgga cgtggtgctc
     1441 gaggtggccc agttcctcga gcgccaggtc gaggtggcgt gcgccgcggg ggtggatccc
     1501 ggcgcgattc tcgtcgaccc gggcatcggg ttcggcaaga cggtcgacca caacctcgag
     1561 ctactgcagc gtctcgacga actccggacc ctcaccgccc agccggtgct cgtggggact
     1621 tcgcgcaagt cggtcatcgg ccaggtcctc ggcctgcccg tggacgaccg gctcgaggga
     1681 accgccgcca ccgtggcctg cgccattgct cggggcgccg acatggtgag ggtgcacgat
     1741 gtgagggaga tgactcgggt ttgccggatg accgatgcag ttcttcgtcg cgaggtgcgg
     1801 atcgcatcat cctccagggg atgatcttcc ggggccacca cggagtctcc gaggccgaac
     1861 ggagtctggt gcagcgcttc cgcgtcgacg tggccctcga gctcgacctg cagcccgccg
     1921 cccgcgccga cgatctcagc cgggcgtgcg actactcccg ggtgttcgag accgtccagc
     1981 acttggtcga gaaacggacc ttccagctcc tcgagaccct cgccgagcgc atcgccgagg
     2041 ccatcctcga cggcttcccc gcggtcaccg ccctgacggt gggtgtcttc aagccggatc
     2101 cacccatgca cggcacattc gagagagtcg gggtcgagat atgccgcacc cggccgtgac
     2161 tcgagcctac ctgggcctgg gcagcaacct gggcgaccgg ttggaggcgc tccgccgggc
     2221 cgtccgcgaa ctcgccacga cacccggcat cgtggttcgc catgtgtcca gctgctacga
     2281 gacggccccg gtcggccccg tgaaccaggg ggacttcctc aacgccgccc tggcgatcgc
     2341 atgggtagag aaggccgctg cggcgggcga cgacgtcgtg a
//
LOCUS       DAOSPL010000726         2379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44679_length_2379_cov_3.014200, whole genome shotgun sequence.
ACCESSION   DAOSPL010000726 DAOSPL010000000
VERSION     DAOSPL010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44679_length_2379_cov_3.014200"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2379
                     /locus_tag="PLQ54_12915"
     CDS             <1..>2379
                     /locus_tag="PLQ54_12915"
                     /inference="COORDINATES: protein motif:HMM:NF019935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOS94212.1"
                     /translation="AEVVMDQRRRLHERPMRRSGLALAALETALIDAAVGEILRRAWD
                     QVDAEGDHCGESKRCPVVIGALGSYGRRELMPYSDVDVLVVAPEGNRPGCDQLVRLVY
                     HGIFDILAQGVKLQIGYSCRSPETPGELDHKTQTALLDLRWVAGSADYLREFEAALDE
                     RFDRAAFLLALASERDQARLDETPELYRIEPDLKRGPGALRDFLSGLSHARAAFRIRG
                     PDSLDQLAAQSVLYDRELRELQDALSQVLGERRALHIVAARPADVLYASLQEQVAAFL
                     GYAGLGGLTGVGQLLRDHYQAARCLHHYSRVLCRRSHAARVRVGGGFIVCEREVRHPV
                     PMEVVSSPELSVAAIALAHAYRVPIGADLADAIRLCADRLEPAKAPSPAAAGLVQILS
                     PDGQPDVAMRYLRDLGLLGRLIPELGRCEDRIPSDPSHEYAILEHSLRVVEQVVALET
                     TADPALAALRKVYTSLSGSARRALLLAALMHDTGKVVDSRDHSTAGAIVTRNVGYRLG
                     LPPAEVDRAARLVEIHLQMAQTSRLKPLHEPGTVLEFREVVRDAETLEQLYLLTYADT
                     KSVGRGVFRDIESHLLEELYAKTLAALADGMQPTDLREAALAITERAVREIRFRDISE
                     DRVREHCQLMPPAYVVSTPMEAIASHIMMVRDLVADRAPVIDAYNPRDAAYTELTVCA
                     YDDPQPGLFAKVCGTLLSNGIDIHSAAIHTRGGPEPIALDTLLIGCGDERLPDDVLQR
                     TVADLRAVLGGQVTVRSLLERRRRVPADAVELISLCLKNPVAQLSPIVLFYL"
BASE COUNT          357 a          904 c          730 g          388 t
ORIGIN      
        1 cgccgaggtc gtgatggacc agcggcggcg gctccacgaa cgtcccatgc ggcgctcggg
       61 cctggccctg gctgccctcg agaccgccct catcgacgcc gccgtcggtg agatcctccg
      121 gcgcgcgtgg gaccaggtcg atgccgaggg cgaccactgt ggcgagtcca aacggtgccc
      181 cgtcgtcatc ggcgccctcg ggagctacgg ccgccgcgag ctcatgcctt attccgatgt
      241 cgatgtgctc gtcgtggcgc ccgagggcaa ccggcccgga tgcgaccaac tcgtgcggct
      301 ggtctaccac ggcatcttcg acatactcgc acagggagtg aagctccaga tcggctactc
      361 ctgccgatcg cccgagaccc ccggcgagct cgaccacaag acacagacag ccctgctcga
      421 tctgcgatgg gtcgcgggca gtgccgacta cttgcgcgag ttcgaggcgg cgctcgacga
      481 gcggttcgat cgcgccgcat tcctgctcgc gctggcctcc gaacgcgacc aggcccgact
      541 cgatgagacg ccagagctct accgcatcga gcccgacctc aaacgcggcc ccggcgccct
      601 gcgcgacttc ctctctgggc tctcgcatgc ccgcgctgct ttccggatcc gcgggccgga
      661 cagcctcgac cagctcgccg cccagtccgt gctctatgac cgcgagctcc gcgagctcca
      721 ggacgcgctc agccaagtcc tcggcgagcg ccgcgcgctt cacattgtgg ccgcccgccc
      781 ggctgatgtg ctctatgcgt ccctccagga gcaggtggcg gccttcctgg gctacgccgg
      841 gctcggcgga ctcaccggtg tgggccagct cctgcgcgac cactaccagg ccgcccgctg
      901 cctgcaccac tacagccgcg ttctgtgccg gcggagccac gccgcccgcg tccgggtcgg
      961 cggcgggttc attgtctgcg agcgcgaggt ccgacaccca gtgccgatgg aggtggtctc
     1021 ctcgcccgag ctctccgtcg ccgccatcgc tctggcccac gcctaccgcg tgcccatcgg
     1081 cgccgacctt gccgacgcca ttcgcctctg tgccgatcgt cttgagcctg ccaaggcccc
     1141 gtctcccgcc gccgccggcc tggtccagat cctcagcccc gacggccagc ccgatgtcgc
     1201 gatgcgctac ctgcgcgatc tgggcctcct gggccggctc atccccgagc tcggccggtg
     1261 cgaggacagg atcccttccg atcccagcca tgagtacgcc atcctcgaac acagcctccg
     1321 ggtcgtcgag caagtggtgg ccctggaaac cacggccgat cccgctctgg ccgctctgcg
     1381 gaaggtctac acatcattgt cgggaagcgc gcggcgcgct ctcctccttg cggcgctgat
     1441 gcacgacacc ggcaaggttg tcgactcacg cgatcacagc accgccggag ccatcgtgac
     1501 ccgcaatgtg ggataccggc tgggcttgcc tcccgccgaa gtcgaccggg ccgcccggct
     1561 ggtcgagatc cacctgcaga tggcccagac ctcacgcctc aagccattgc acgagcccgg
     1621 caccgtcctc gagttccgcg aggtggtccg cgacgccgag acgctcgaac agctctacct
     1681 gctcacctat gccgacacca agtcggtcgg ccggggcgtc ttccgggata tcgagagcca
     1741 cctacttgag gaactctacg ccaagactct ggccgccctc gccgacggca tgcagcccac
     1801 cgacctgcgg gaggcggccc tggccatcac cgagcgcgcg gtccgcgaga tccggttccg
     1861 cgatatctcc gaggaccgcg tacgcgagca ttgccagctc atgcctcccg cctacgtcgt
     1921 cagcacaccc atggaagcca tcgcctctca catcatgatg gtgcgtgacc tcgtggccga
     1981 ccgggcgccg gtcatcgatg cgtataaccc gcgagacgcc gcctacacgg agctcacagt
     2041 ctgcgcgtac gacgatcccc agccgggcct gttcgcgaag gtctgcggta cgctgctctc
     2101 gaacgggatc gacatccact cggccgccat ccacacccgc ggcgggcccg agcccatcgc
     2161 gctcgataca ctgctcatcg ggtgcggcga tgaacgcctg cccgatgacg tcctccagcg
     2221 caccgtcgcc gatctgcgtg cggtgctggg gggccaggtg accgtccggt cgctgctcga
     2281 acggcgacga cgcgtgcccg ccgatgcagt cgagctgatc agtctctgcc tgaagaaccc
     2341 ggtggcgcaa ctttcaccca tcgttttgtt ttacttaaa
//
LOCUS       DAOSPL010000727         2377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44725_length_2377_cov_3.066322, whole genome shotgun sequence.
ACCESSION   DAOSPL010000727 DAOSPL010000000
VERSION     DAOSPL010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44725_length_2377_cov_3.066322"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PLQ54_12920"
     CDS             complement(<1..343)
                     /locus_tag="PLQ54_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94213.1"
                     /translation="MSETTTETTTPTPKAVAEAAEKGRPGETPEQTIEALRSALAKAN
                     DEAKENRLKATELDQIKHAQMSELEKAQAAMQTATQEAAAAKAEALRWRIAAKHGISD
                     EDAETFLTAGDE"
     gene            complement(390..542)
                     /locus_tag="PLQ54_12925"
     CDS             complement(390..542)
                     /locus_tag="PLQ54_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94214.1"
                     /translation="MTKLDLEFYRDAIRNGRTPNIPAHAIEDVFAGLDDRTVKAYLAR
                     RRRGSH"
     gene            complement(539..2176)
                     /locus_tag="PLQ54_12930"
     CDS             complement(539..2176)
                     /locus_tag="PLQ54_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94215.1"
                     /translation="MLPRAVSDHYQTQQRLIVATLGLSRSEWSAMRLDDIDSSWAKVG
                     PRVNLLTASAQLGAARGGAAYVAATLAEYGDAIPPLAQVNPGAFAGIASDGRPLGSLL
                     EGAKIRAKEAQSLAAGGKWLDMAIHTQIADAGRAAAATAIAVRPGVGWVRMVNPPCCG
                     PCAVLAGREYRYSQGFQRHPRCDCIHVPTTLANPGSHDGVDPTFDQITDLTEGERKAL
                     MEGADLSRVVNARRDGGLGKMSTTELATNGRARLTPDGIFAQAKGRDEALALLKSHGY
                     ITSTARRVASAPAVPVVAAPTRPVIRFGKALTAPDGVAKPLKGHAPGRPLTVEEWEQA
                     PSHKADYDFRLISDPDERATGVLQYDTYTERWAKTAAANIKAGRDPFDGISDATLDSE
                     YLHYQRILDKRNFPDGTGYEMGDLRADLEGTARKLIEWESRTEDLGGAFKGVNFDADL
                     SWDDVLARLNMRGLNYTSVASERYTASMYAGRADRCRSVVLEFEDARGIRLDSVGAHA
                     SKTESLVSGDYEMVGYRIEADPDGYGERMFVQVRRAT"
     gene            complement(2154..>2377)
                     /locus_tag="PLQ54_12935"
     CDS             complement(2154..>2377)
                     /locus_tag="PLQ54_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012577467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94216.1"
                     /translation="DPSTPTIAQREDALMKRRSVGVLSREGYWDELGWSEPRKAKERQ
                     YLDEEESSDPIPNAARSLTGGAGATAGGL"
BASE COUNT          331 a          800 c          796 g          450 t
ORIGIN      
        1 cttcgtcgcc agcggtgagg aacgtctccg cgtcctcgtc gctgatgccg tgcttggccg
       61 cgatgcgcca cctcagagcc tccgccttcg cggccgcggc ctcctgggtg gcggtctgca
      121 tcgccgcttg ggccttctca agctcactca tctgcgcgtg cttgatctgg tccagctcgg
      181 ttgccttgag acggttctcc ttcgcctcat cgttggcctt ggccagcgcc gaccgcagag
      241 cctcgatggt ctgctcgggc gtctcgccgg gcctgccctt ctcggccgcc tccgcgaccg
      301 ccttgggtgt gggcgtggtc gtctcggtcg tcgtctccga catgttctct ccctcgcgga
      361 gaaagtcccc aacctcgcgt cgggggtggc tagtggctgc cgcggcggcg gcgagcgaga
      421 tacgccttga cggtgcggtc gtctagacct gcgaacacgt cctcaatggc atgggctggg
      481 atgttgggcg tccggccgtt gcggatggcg tctcggtaga actcgaggtc aagtttcgtc
      541 atgtggccct ccgcacttga acgaacatgc gctcgccata gccgtcgggg tccgcctcga
      601 tccgatagcc gaccatctca tagtcgcccg agacgagcga ttccgtcttg gatgcgtgcg
      661 cgccaaccga gtcgagccga atacctcgag cgtcctcgaa ctccagcacc acagaccggc
      721 agcggtcggc gcgtccggca tacatggagg ctgtgtaacg ctcggaagca acagaggtgt
      781 agttgagtcc gcgcatattc agtcgcgcta gaacgtcgtc ccatgacaag tccgcgtcaa
      841 agttgacgcc cttgaacgcc ccgcccagat cctcggtacg cgactcccac tcgatgagct
      901 tgcgggcggt cccctcgagg tccgctcgca agtcgcccat ctcgtatcca gtgccgtccg
      961 ggaagttgcg cttgtccaag atcctctgat agtgcagata ttcgctatca agggtggcgt
     1021 cgctgatccc gtcgaagggg tcgcggcccg ccttgatgtt cgcggcggca gtcttggccc
     1081 accgttcggt gtaggtgtcg tattgaagga cacccgtagc ccgctcgtcg gggtcagaga
     1141 tgaggcggaa gtcgtagtcg gccttatggg atggcgcctg ctcccattcc tcgacagtca
     1201 gcggtcgccc tggagcgtgt ccctttagtg gtttggcgac accatccggc gcagtgagcg
     1261 ccttgccgaa gcggattacg ggccgggtcg gtgctgcgac taccgggacg gccggcgcgg
     1321 aggcgacgcg acgggcggtg gacgtgatgt agccgtgcga cttgaggagc gcgagcgcct
     1381 cgtcgcggcc cttggcttgg gcgaagatcc cgtcaggggt gagccgcgct cgaccattcg
     1441 tcgcgagctc ggtcgtggac atcttgccga gcccgccgtc gcgtcgggcg ttgaccactc
     1501 gggagagatc cgcgccctcc attagcgcct tgcgctcgcc ttcggtcagg tctgtgatct
     1561 ggtcgaacgt cgggtccacg ccgtcgtgac tgcccgggtt ggcgagcgtt gtcgggacgt
     1621 gtatgcagtc gcagcgcggg tggcgctgga agccttggct gtagcggtac tcgcggccgg
     1681 cgaggaccgc acacgggcca cagcacggcg gattcaccat gcgaacccac ccgacgcccg
     1741 ggcggactgc gatggccgta gcagccgctg cacggcccgc gtcggcgatc tgcgtgtgga
     1801 tcgccatgtc gagccacttg ccgccagccg cgagcgactg cgcctctttc gcgcggatct
     1861 tcgcgccctc gagcagcgag cccagcggcc gcccgtccga cgcgatcccg gcgaaagcgc
     1921 ctgggttcac ttgggccagc ggcgggatgg cgtcgccgta ctccgcgagt gtcgctgcga
     1981 cgtaggccgc gccgcccctt gcggctccga gttgggcgga ggccgtgagc aggttgaccc
     2041 gcgggccgac cttcgcccac gacgagtcga tgtcatcgag cctcatcgcg gaccactcgg
     2101 aacggctcag ccccagcgtc gcgacgatga gccgctgctg cgtctggtag tggtcagaga
     2161 ccgcccgcgg tagcacccgc gccacccgtc aggctgcgag ccgcgttggg gatcgggtcc
     2221 gacgactcct cttcgtcgag atactggcgc tccttggctt tccgcggctc tgaccagccg
     2281 agctcatccc agtagccctc cctggacagc acaccgacgc tacgccgctt catcaaggcg
     2341 tcctcgcgct gagcgatcgt cggcgtggac gggtcga
//
LOCUS       DAOSPL010000728         2376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44742_length_2376_cov_4.411030, whole genome shotgun sequence.
ACCESSION   DAOSPL010000728 DAOSPL010000000
VERSION     DAOSPL010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44742_length_2376_cov_4.411030"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..980)
                     /locus_tag="PLQ54_12940"
     CDS             complement(78..980)
                     /locus_tag="PLQ54_12940"
                     /inference="COORDINATES: protein motif:HMM:NF014601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HOS94217.1"
                     /translation="MARVRVLGAGVVGSVYAALLADAGHEVTLIARGSRLVDLRRHGL
                     LVGLDGRILAPDVAVTKSPEVTDCDVLLVAVRGDQLAAALDDIAASPAGWVWMFTNPL
                     ELRSDVEDRLGRDRLVWCFSGVGGGVKDGQVTAHRVGQQPTVVEGGAAGSKFASQLLG
                     AIDPHLRTEPDMHAWLDTHTVFVAAMAALVLEQPLETRSQRVRGAVLLVRAMRQAFDS
                     LEAQGARITPRNLRMIFGRVPLPIAALYWAAQLSRPVVQVSMLPHATATRTTEQRAVA
                     AHALDLAGHGPGLYRQLLAPLAGT"
     gene            complement(1090..1425)
                     /locus_tag="PLQ54_12945"
     CDS             complement(1090..1425)
                     /locus_tag="PLQ54_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94218.1"
                     /translation="MNEYSWEGISPEAQFLWGERTKEIGDRPVDSDDANDLPLARLAR
                     EHGFEVAPDAPMWLLLPVVWPIADRAWIRDNRLRHLQTYVDGQLVDVPWSTADYLEAA
                     DDEHDSLGN"
     gene            complement(1678..2256)
                     /locus_tag="PLQ54_12950"
     CDS             complement(1678..2256)
                     /locus_tag="PLQ54_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94219.1"
                     /translation="MRFRVSAPTSRVLGTWRAPWWQRIIFPAVGAALALGSLWPYAGE
                     RYEPLWWGLPLGALFVTYPLRPRLRLHEDALHVRGVVFSRVIPIADVAAVQGGYGGLG
                     IWWGDGRFTEASTIGEQNNVEWLPGGDSRRYSMRDLIFETRDAYLLEHGLEARPDPHH
                     ELEGQRREFQERGWVEHNPPLLPKTDRDDKRP"
BASE COUNT          412 a          818 c          769 g          377 t
ORIGIN      
        1 ttgactcccc cctggaccga tcagcggcag gagcgcctta cctctccgcg gcaaagtagt
       61 gcgttccgag cacccgctca ggtgccggcc aaaggagcga gcagctggcg gtagaggccg
      121 gggccgtggc ccgcgaggtc cagggcgtga gcggccacgg cccgctgttc ggtggttcgg
      181 gtcgcggtgg catgggggag catggacacc tgcacgacgg gtcgtgacag ctgcgcagcc
      241 cagtacaggg cggcgatcgg caggggcacc cggccgaaga tcatccgcag gttgcgcggt
      301 gtgatgcgag ccccctgcgc ttcgagggag tcgaaggcct gtcgcatggc ccgaaccagc
      361 aggacagcgc cgcgcactcg ctgtgatcgc gtctccaagg gctgctccag gaccagggcg
      421 gccatggcgg ccacgaacac cgtgtgggtg tccaaccagg cgtgcatgtc cggctcggtc
      481 cgcaggtggg ggtcgatcgc gccgagcagc tgcgacgcga acttggagcc ggctgccccg
      541 ccctcgacca cggtgggctg ctgccccacc cggtgggcgg tcacctgccc gtccttcacg
      601 ccgccgccca caccggagaa gcaccagacc agccggtcgc ggcccaggcg atcctccacg
      661 tcactgcgca gctcgagggg gttggtgaac atccagaccc acccagccgg gctggctgcg
      721 atgtcgtcca gggccgcagc caactggtca ccgcgaacgg cgaccagcag gacgtcgcag
      781 tctgtcactt ccggtgactt cgtcacggcc acgtcgggcg caaggatccg gccgtcgagg
      841 cccacgagca gtccatggcg gcgcaggtcg acgagtctcg atcctcgtgc gatcagggtg
      901 acctcgtgac cggcgtcggc cagcagcgcc gcgtagaccg atccaacgac acctgccccg
      961 agcaccctca cacgcgccat agtgcgagcc tatgcgccag gaggacccag aagtgccggt
     1021 gcgccaccgg cggcatgtgt ggatgttcag cctgcggtcc agatcttcat cactgcggcc
     1081 acttgctcat caattcccta gggagtcgtg ctcgtcgtcg gcggcctcga ggtagtccgc
     1141 cgtggaccac ggaacgtcaa ccagctgacc atcgacgtag gtttgcagat gacgcaggcg
     1201 gttgtcccga atccatgccc gatccgcgat tggccacacc accgggagaa ggagccacat
     1261 cggagcgtcc ggagctacct cgaacccgtg ctccctcgcc agacgagcga gcggaaggtc
     1321 gttcgcgtcg tcactgtcaa ctggacggtc gccgatttcc ttcgttcgct ccccccaaag
     1381 gaactgcgct tcgggcgaga tgccctccca cgagtactcg ttcacgaggg acagcctctc
     1441 agtgacggtt ccgcgggtac agcacccgcg atgcggcatg acaggacgag ttcggccaag
     1501 gtggcggcgc gactccgggg tctacttcca gcccttctcg aacccatacg ggacgacccg
     1561 tgaatcgcct taccggctgg acggccgccg agtggagggt agcgactggc ggcacttcac
     1621 ggcgcggcca tttcccacgc cgatcttgat acgcaccaac cgcggcatga gcgggcgtca
     1681 tggcctcttg tcgtcgcggt cagtcttggg cagtagcggt gggttgtgct cgacccatcc
     1741 acgttcttgg aactcccggc gctgcccctc cagttcatgg tgcggatccg gccgcgcctc
     1801 gaggccatgc tccagcaggt acgcgtcgcg agtctcgaag atgaggtcac gcatggagta
     1861 gcgacggctg tcaccgccag ggagccattc gacgttgttc tgctccccga tcgtgctcgc
     1921 ctccgtaaag cggccatcgc cccaccagat gcccaggccg ccgtatccgc cctggacggc
     1981 agccacgtcc gcgatgggta tgacccggct gaagacgacc ccgcgcacgt ggagagcgtc
     2041 ctcgtgaagc ctcagtcgtg gcctcaatgg ataggtgacg aatagcgctc caagcggcag
     2101 accccaccac aaaggctcat agcgctcgcc cgcgtacggc cagagcgacc caagcgccag
     2161 agccgcaccg actgcgggaa agataatgcg ctgccaccac ggagctcgcc atgttccaag
     2221 gactcgcgag gtaggagcgg aaactctaaa tcgcacgggc gaccctctcg gtaggactct
     2281 ccgagcatct cactctgctc ggttcagccg agccaccacg cccgctcatc ggctacttgt
     2341 ttcattgacg gcatggccgg tatctgacga ggctcg
//
LOCUS       DAOSPL010000729         2374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44803_length_2374_cov_2.701164, whole genome shotgun sequence.
ACCESSION   DAOSPL010000729 DAOSPL010000000
VERSION     DAOSPL010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44803_length_2374_cov_2.701164"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PLQ54_12955"
     CDS             <1..769
                     /locus_tag="PLQ54_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOS94220.1"
                     /translation="CSWLNRMHYRQVMPYALGRAARIFCPSCATRDVLVQRLRVGESR
                     IVVVPLGVERRFRRILQPDVLEQVRTALELPEQFVLFVGNLEPRKGLPRLLEAFIRAR
                     KSTGFPHKLVIAGKPGDSAKAVMHAVDAPHAREHVMALGYVSDACLPHLYNLATAVIC
                     PAPFEGFGFTALEAMACGTPVLANESGALPEVCGDAALYVQPRHVEPLAEAIRRILSD
                     EELRHRLIAQGLTRAERFQWATTARVTLATYEEVFGG"
     gene            762..1988
                     /locus_tag="PLQ54_12960"
     CDS             762..1988
                     /locus_tag="PLQ54_12960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS94221.1"
                     /translation="MADRIAYVLGRFPVLSETFIAREIRDLLEQGVAIDVYALVRQPQ
                     GCAPPEAERFAEAVTFRPPLPRLAAAHPLRLARHLLAREGRQLRSEARTGGAGWGMLA
                     AGRVASACWLADRVGRSGAGHIHAHFLGAPAQVAYLASRILRLPFSVSAHASDAFVRG
                     RVTAFERRIAGEAAAIVTCSFSLAEHLVLAHGLPSEKLLPIYHGIRAEELQVDRRPQA
                     PPVVLAVGRLVEKKGFHHLITAMALLRKAGHRFRCSIVGEGPERPLLESLIADARLGD
                     QVELLGARPFRDVVRLYGCASVLVAPSIEATDGDMDGLPNVLLEAAAARVPIVATTVS
                     AIPELIRHEATGLLVSPHDAHGLAEAVGRILSDDALARDLAEAAHERVLRHFDSRESA
                     RRLAKLFETQSGAGPK"
     gene            2260..>2374
                     /locus_tag="PLQ54_12965"
     CDS             2260..>2374
                     /locus_tag="PLQ54_12965"
                     /inference="COORDINATES: protein motif:HMM:NF014458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94222.1"
                     /translation="MLDIRLFRDQPDAVREGLRSRGRDSSVVDEVIRLDAQR"
BASE COUNT          324 a          824 c          815 g          411 t
ORIGIN      
        1 ttgctcgtgg ctcaaccgga tgcattatcg ccaggtgatg ccgtacgccc tggggcgtgc
       61 ggcccggatc ttctgcccct cctgtgccac ccgcgacgtg ctcgtgcagc gcctgcgcgt
      121 gggcgagtca cggatcgtcg tggttcccct cggggtcgag cgtcgtttcc gtcgtatcct
      181 ccaacccgat gtgcttgagc aagtgcgcac cgctctggag ctaccggagc agttcgtgct
      241 gttcgtcggc aacctggagc cccggaaggg cctgccgaga ctgctggagg ccttcatccg
      301 cgcgcgcaag tcgaccggct tcccacacaa gctggtgatc gcaggcaagc ccggcgactc
      361 ggccaaggcg gtgatgcacg ccgtcgacgc cccacacgcc cgggagcacg tcatggccct
      421 gggctatgtg agcgatgcct gccttcctca tctgtacaac ctggcaaccg ctgtcatctg
      481 cccggcaccc ttcgagggct ttggattcac ggccctcgag gccatggcgt gcggcacgcc
      541 ggtcctggcc aacgagtctg gcgccctccc ggaggtctgt ggggatgctg ccctgtacgt
      601 ccagccgcgc cacgtcgagc ccctggccga agcgatccgc cgcatactga gcgacgagga
      661 gctccggcac cgtctcatcg ctcagggcct cacccgggca gagcgcttcc agtgggcgac
      721 cacggcccgg gtcaccctgg ccacctacga ggaagtgttc ggtggctgac cgcattgcct
      781 atgtgctcgg ccgcttcccc gtgctcagcg agaccttcat cgcccgcgag atccgcgact
      841 tgctcgagca gggagtggcc atcgatgtct acgcgctggt gcgccaaccc cagggctgcg
      901 cgccgcccga ggccgagcgg ttcgccgaag cggtcacttt ccggcccccg ctgccgcggc
      961 tggcggcggc acatccgctc cgcctcgctc gtcatctgtt ggctcgcgag gggcgccagc
     1021 tccggagcga ggcacggacc ggtggggcgg ggtgggggat gttggcggcc gggcgcgtcg
     1081 cgtcggcgtg ttggctggcc gaccgcgtgg ggcggagcgg ggccgggcac atccatgcgc
     1141 acttcctggg tgcgccggcc caggtagcgt acctggcttc gcgaatcctt cgcctgccct
     1201 tctcggtatc ggcacacgct tcggacgcct ttgtgcgcgg acgcgtcacg gcgttcgagc
     1261 ggcggatcgc cggcgaagcg gccgccatcg tcacgtgcag cttcagcctg gccgaacacc
     1321 tcgtgttggc ccacggcctg ccctccgaaa agctgctgcc catctaccac gggatccggg
     1381 ccgaggaact gcaggtcgac cgtcgtcccc aagcgccccc cgtggtactg gccgtcggcc
     1441 ggctcgtcga gaagaaaggc ttccatcacc tgatcacggc catggccctg ctccgcaagg
     1501 cgggacatcg attccgttgc tccatcgtgg gcgaggggcc cgaacggccg ctgctggagt
     1561 ccctcatcgc ggatgctcga ctcggtgatc aggtcgagct gttgggcgca cggcccttcc
     1621 gggacgtggt ccgcctctat ggttgcgcat cggtgctggt ggcgccgagc atcgaggcca
     1681 ccgatggaga catggatggg ctaccgaacg tgctactcga ggctgcggcc gctcgcgtgc
     1741 ccattgtggc cacgaccgtg tccgccatcc ccgagctcat ccgccacgaa gccacggggc
     1801 tgctggtgag cccgcatgat gcccacggcc tcgccgaggc cgtgggccgt atcctgtccg
     1861 acgatgcgct ggcccgggat ctcgccgagg ccgcgcacga gcgcgttcta cgccacttcg
     1921 actcgcgcga atccgcgcgg cgtctggcca agctgtttga aacccagtcg ggggccgggc
     1981 cgaaatagcg gaaaagccat cgcgccggaa ccgcacggtc gcttttcccc cgcgggcggc
     2041 ccgggtggcg ggaactcgac gccgtctgat ggggccctac cgggcacgag gttctctgtg
     2101 tttggcaaga ctcacgggtt ggtggaggaa cgaacgggcg ctggccggca ctgcccgtgt
     2161 tggcccgcgg tccagcgcct gtgctatcat cagccagcgg tggggatcct gagcgtcctc
     2221 cggggcgtcg gatcccatgg atgagggagg actggtggca tgctcgacat ccggctgttt
     2281 cgcgatcagc ccgacgcggt gagggagggg ctccgttcgc gcggccgcga cagttcggtg
     2341 gtcgacgaag ttatccgcct cgatgctcag cgcc
//
LOCUS       DAOSPL010000730         2370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44900_length_2370_cov_3.565443, whole genome shotgun sequence.
ACCESSION   DAOSPL010000730 DAOSPL010000000
VERSION     DAOSPL010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44900_length_2370_cov_3.565443"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1671
                     /locus_tag="PLQ54_12970"
     CDS             <1..1671
                     /locus_tag="PLQ54_12970"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOS94223.1"
                     /translation="GWTGADFDNSAWPAAEAAYRAPGGPWGVPDVYPLRQPVDRATPE
                     VTIYAGGDGLWTMLRPLTPLLAPLRFSVGVSALDADSVAGLGQMTDGELVPGADPLRV
                     QLAVPEGTSGPVAATVSFGPDGAQVTKVAWLPGAPAPAADEPAQCAEATGVFRAERVG
                     DRWWLIDAHGRPFWSLACNAVMHEPYWSFHYSRLVANRYVDERSWQEVTVDRLCQWGF
                     NSSSGSPADEPWARRGRPYFAGWNLTWAGPRLQDADGRPILFPDVFDPDWQRGAEGRV
                     RETTERYKDDPLLIGYWTDNEIQMHDPMSPSLGIMGSFWSPGCRKELSRWLSERYGGD
                     IEALNRRWSSREHTYAYGSFEDLPADKPQIRGAGDPVAPDLRDFVRHIIGTYAETYVR
                     LWKKHDPRHLVCSNRFAGQFNLDFADLLKPFDIIACNSYPRDHWGQTEFDRGQLQWLR
                     RMHQITGRPVLISEWGTQARDSRLCNNRGRLDTQAQRGECYERVMHQLWDEDYIVGAH
                     WFCWADSTDGERANWGLVDAFDRPYLELTSSARRANLWLTDQVRQWEP"
     gene            complement(1678..>2370)
                     /locus_tag="PLQ54_12975"
     CDS             complement(1678..>2370)
                     /locus_tag="PLQ54_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS94224.1"
                     /translation="DPLAPAKWEGFAAQVAAGAGKFVQFGIGFSLFERAWTLRGMENL
                     FLDMVEAPEFVHELLDAICDYNVALAGRALEFDIDAVHFGDDWGSQRGLLMGPGLWTE
                     FILPRLERQYAVAKAAGRFVTIHSCGKVQEVFPQLIDIGLDCFNPFQPEVMDVYEMKR
                     QFGDRLSFWGGVSTQKLLPYGTPDEVRAEARRMMAEVGRDGGFIIAPAHAIPGDAKPE
                     NIMALIETVNDQ"
BASE COUNT          367 a          832 c          797 g          374 t
ORIGIN      
        1 ggctggactg gggccgactt cgacaactcc gcttggcccg cggccgaggc ggcgtaccgc
       61 gcgcccggcg ggccgtgggg cgtgcctgac gtgtacccgt tgcggcagcc cgtcgaccgg
      121 gccacgccgg aggtcacgat ctacgccgga ggggacggcc tctggacgat gctccgcccc
      181 ctgacccctc tgctggcccc gctgcggttc tccgtcggag tcagcgcgct cgacgccgac
      241 tcggtcgccg ggctgggtca gatgaccgac ggcgagttgg tgcccggcgc cgatccgctc
      301 cgcgtgcaac tggctgttcc cgaaggcacg tcgggcccgg tcgcggccac ggtgtcgttc
      361 ggccctgacg gcgcgcaggt caccaaggtc gcttggctac ccggggcgcc ggcgccggcc
      421 gccgacgaac cggcccagtg cgccgaggca acgggcgtct tccgcgcgga gcgggtgggc
      481 gaccggtggt ggctcatcga cgcccatggc cgaccgttct ggagcctggc ctgcaatgct
      541 gtcatgcacg agccgtactg gtcgttccat tactcgcgcc tcgtagcgaa ccggtatgtc
      601 gatgagcgtt cgtggcagga ggtgaccgtc gatcgcctgt gccagtgggg gttcaacagc
      661 agcagcggat cgcccgccga tgagccctgg gcgcgacgcg ggcgccccta cttcgcgggg
      721 tggaacctga cctgggccgg ccccagactt caggacgccg atggccggcc gatcctgttc
      781 cctgatgtct tcgatcccga ttggcagaga ggcgccgagg ggcgagtccg cgagaccacg
      841 gagcgctaca aggatgaccc cctgctcatc gggtactgga ccgacaacga gatccagatg
      901 cacgacccga tgtctccctc gctgggcatc atgggcagct tctggtcgcc ggggtgccgg
      961 aaggagctct cccgctggct ttccgagcga tacgggggcg acatcgaggc gctcaaccgc
     1021 aggtggtcgt cgcgggagca cacctacgcc tacggctcgt tcgaggacct gcccgcggac
     1081 aagccgcaga ttcgcggcgc cggcgatccg gtggcgcccg atctgcgcga cttcgtccgc
     1141 cacatcatcg gcacgtatgc ggagacgtac gttcggctgt ggaagaagca cgacccgagg
     1201 cacctggtct gcagcaaccg gttcgcgggg cagttcaacc ttgacttcgc cgacctgctg
     1261 aagccctttg acatcatcgc ctgtaacagc tacccgcgcg accactgggg ccagacggag
     1321 ttcgaccgcg gccaactgca atggctgcgg cggatgcacc agatcaccgg ccgccccgtc
     1381 ctcatcagcg agtggggcac tcaggcgcgc gactcacgtc tgtgcaacaa ccggggccgg
     1441 ctcgacaccc aggcgcagcg cggcgagtgc tacgagcgcg tcatgcacca gctctgggac
     1501 gaggactaca tcgtgggcgc ccactggttc tgctgggccg actccaccga cggcgagcgc
     1561 gccaactggg gcctcgtgga cgcattcgac cggccctacc ttgaactcac gtcgtcggcc
     1621 cgccgcgcca atctgtggct cacagaccag gtgcgccagt gggagccgta gctcgcccta
     1681 ctggtcattc accgtctcga tcagcgccat gatgttctcc ggcttggcgt cgccggggat
     1741 ggcgtgcgcg ggggcgatga tgaacccgcc gtcgcgcccc acttcggcca tcatcctccg
     1801 ggcctcggcc cgcacctcgt cgggtgtgcc gtagggcagc agcttctgcg tgctcacgcc
     1861 gccccagaag ctcagccggt cgccgaactg gcgcttcatc tcgtagacgt ccatgacttc
     1921 tggctggaag gggttgaagc agtccagccc gatgtcgatg agctgcggga acacctcctg
     1981 caccttccca caggagtgaa tcgtgacgaa ccggcccgcc gccttggcca ccgcatactg
     2041 gcgttcgagc cgcggcagga tgaactcggt ccagagaccg gggcccatga gcagcccgcg
     2101 ctgcgagccc cagtcgtcac caaagtggac tgcgtcgatg tcgaactcga gcgcccgccc
     2161 ggccagcgct acgttgtagt cgcagatggc atcgagcagc tcgtggacga actccggcgc
     2221 ttcgaccatg tcgaggaaca ggttttccat gccccgcagg gtccaggcgc gctcgaacag
     2281 cgagaagccg atcccgaact gcacgaactt gcccgcgccg gcggccacct gggcggcgaa
     2341 accctcccac ttggccggcg ccagcgggtc
//
LOCUS       DAOSPL010000731         2370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44903_length_2370_cov_3.435421, whole genome shotgun sequence.
ACCESSION   DAOSPL010000731 DAOSPL010000000
VERSION     DAOSPL010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44903_length_2370_cov_3.435421"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="PLQ54_12980"
     CDS             complement(<1..1021)
                     /locus_tag="PLQ54_12980"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HOS94225.1"
                     /translation="MSSRRSNVRALIERIPKAELHLHLGGAIPPHVLIELLDTHRPTQ
                     WLGDIPERHQRLFRETPNIRPFLSMERPGASELASLFVYASFHQFLATFLFTSYLIRQ
                     VDDLRCLIAGVAESLHAQGIVYAEITVDIMGYVRRGLCLEQVGACLAEGAARDDVCIR
                     WIVDPVRDFGPEGTLAHLRQVLDLRCPGLVGMTIGGSEHLFPPRQFGPLYDLAREHGL
                     RLTVHAGEALGPESVWDALRCLRPHRIGHGVRSVEDPALMAHLAEARVPLEVCPTSNL
                     RTAVYASYAEHPLPQLYRAGVPISLSTDDPTFFETTLAEEYLHAYDMGLGLDDHGLVG
                     KLQRLD"
     gene            complement(1002..>2370)
                     /locus_tag="PLQ54_12985"
     CDS             complement(1002..>2370)
                     /locus_tag="PLQ54_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94226.1"
                     /translation="TVSLTGDGAARDVDRALFCADRHRRTFARMSRFLREELGVRALL
                     TDMNAWTENIAHQVVRTDFDWVDTHFYWDHPNFLERSWSLPSRGWSGGECAVSARGAG
                     ARDQALLRLWGKPFTISEFNFVAPNEYRSEGGLLIGAIAGLQDWDGLYRFAYSHDQRN
                     LSETRPVGFFDVASDPLSLASDRVGLLLFLRGDVKAAPHSVALPIDVQSTLDRPPGLP
                     DLHGQITELAYITRVGTAVGPSDADIVLGGLPGVSGVTLDVQPTAADASARVVADLRR
                     RGWLDAANPTDVGQGILASETGELVLDAEGGTLSIATPRTAAVFGRDPGVELIAGGLR
                     VTLQGTGGAVVASALDGRPLAESRRVLVMHLTDVRNSGMRFRDKDQKILEAWGESPYL
                     VRAGSAAISLAGARGDGLRAWALRSDGSRVGEIELRQAADSVSFVANTRGPEGGRLSY
                     ELAAE"
BASE COUNT          399 a          790 c          833 g          348 t
ORIGIN      
        1 catcgaggcg ctgcagtttg ccgacaagac cgtgatcgtc gagtcccagg cccatgtcgt
       61 aggcgtggag gtactcctcg gccagtgtcg tctcgaagaa ggtcgggtcg tctgtgctca
      121 ggctgatcgg cacgccggcg cggtagagct ggggcagcgg gtgctcggcg taggaggcat
      181 agacggccgt ccgcagattg ctggttggac agacttcgag cgggacgcgg gcttcggcca
      241 ggtgagccat tagggctggg tcctcgacgc tccgcacgcc gtggccgatg cggtggggcc
      301 ggaggcaacg cagtgcgtcc caaacgctct ccggccccag ggcctccccg gcgtggactg
      361 tcagccgtag gccgtgctcc cgggccaggt cgtagagcgg cccgaactgc cgcggtggga
      421 acaggtgctc actgccgccg atcgtcatcc caacgagtcc ggggcagcgc aggtcgagca
      481 cctggcgcag gtgagcgagt gtgccctccg gaccgaagtc gcgcacggga tcgacgatcc
      541 agcggatgca cacgtcgtcg cgtgcggccc cctcagccag acacgcacct acctgctcga
      601 ggcacagccc gcggcgcacg tagcccatga tgtcgacagt gatctcggcg tagacaatgc
      661 cctgtgcgtg gagcgactcg gccacgcccg caatgaggca gcggaggtca tccacttggc
      721 ggatcaggta gctcgtgaac aggaaggtcg ccaggaactg gtggaagctg gcatacacga
      781 acaggctggc gagctcgctc gccccggggc gctccatcga caggaacgga cggatgttgg
      841 gcgtctcgcg gaagagtcgc tggtgccgct cgggtatgtc ccccagccac tgggtgggtc
      901 ggtgagtgtc gaggagctcg atcagcacgt gcggcggaat ggcgccgccg agatggaggt
      961 gcagctcggc cttcgggatg cgctcgatga gcgcgcggac gttactccgc cgcgagctca
     1021 taggagagcc gtccgccctc agggccgcgc gtgttggcga cgaagctcac actgtcggcc
     1081 gcctgtcgga gctcgatctc acccacccgg ctgccatcgc tccgcagggc ccaggcacgc
     1141 agcccgtcgc ccctggcgcc ggcgaggctg atcgccgccg acccggcgcg cacgagatag
     1201 ggagactcac cccacgcctc gaggatcttc tggtccttgt cgcggaagcg catgccggag
     1261 ttccgcacat cggtgaggtg catgacaagc acccggcggc tctcggccag gggccggccg
     1321 tccagtgcgg acgccaccac ggcaccaccg gtgccctgga gagtgacgcg cagcccgccc
     1381 gcaatgagct cgacgccggg atcacggccg aacacggcgg cggtgcgcgg cgtggcgatg
     1441 gacagcgtac cgccttcggc gtccagaacc agctcgccgg tctcggaggc cagaatcccc
     1501 tggccgacat ccgttgggtt cgccgcgtcg agccatccgc gccgacgcag atcggccacg
     1561 acgcgcgcgc tggcatcagc ggccgtgggc tggacatcga gcgtcactcc cgagacaccg
     1621 ggcagtccgc cgagcacgat gtcggcatca ctggggccga ccgcagtgcc gacccgtgtg
     1681 atataggcga gttcagtgat ctgcccgtgc agatccggta gcccaggcgg tctgtcgaga
     1741 gtggattgga catcgatcgg cagagcgaca ctgtgaggag ccgccttcac atcgccgcgg
     1801 aggaacagca gcaaccccac ccggtccgag gccagtgaca gcggatcgct cgcgacatcg
     1861 aagaagccta ccgggcgcgt ctcggagagg ttccgctggt catggctgta ggcgaaccgg
     1921 tagagcccat cccagtcctg gagcccggcg atcgcgccta tgagcagacc cccctccgag
     1981 cggtactcgt tgggggcgac gaagttgaac tcggagatcg tgaacggctt accccacagc
     2041 cgcagcaacg cctggtcgcg cgcaccggca ccgcgggccg agacggcgca ttcgccgccg
     2101 ctccagcctc gcgaaggcag tgaccaggat cgttcgagga agttgggatg gtcccagtag
     2161 aagtgcgtgt cgacccaatc gaagtccgtg cggaccacct ggtgcgcgat gttctcggtc
     2221 caggcgttca tgtcggtcag cagggcccgg acacccagct cctcgcggag gaagcggctc
     2281 atcctggcga aggtcctccg gtggcggtcg gcgcagaaca gcgcccggtc gacatcccga
     2341 gccgcgccgt cgcccgtcag cgatacggtg
//
LOCUS       DAOSPL010000732         2370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_44908_length_2370_cov_3.041469, whole genome shotgun sequence.
ACCESSION   DAOSPL010000732 DAOSPL010000000
VERSION     DAOSPL010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44908_length_2370_cov_3.041469"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLQ54_12990"
     CDS             <1..405
                     /locus_tag="PLQ54_12990"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOS94227.1"
                     /translation="EGFVLVQARSDAEWFAAHLEGVALVERWCLDSLTDLGAMVGGTI
                     RATGGGSRSDIWLQVRAHVLQRSLERPAVADGAMGSALLAASRTLFDGLTAAARAMVR
                     IAKRVEPDAALGPCYADKLDQLRERCRELGYL"
     gene            427..1854
                     /locus_tag="PLQ54_12995"
     CDS             427..1854
                     /locus_tag="PLQ54_12995"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOS94228.1"
                     /translation="MDPFLDCDIRGVYGHEITELFARDLGLAVGSVAGRGPVVVAGDY
                     RTHTPVLMAALCDGLRQAGADVVSCGQIPTPVYYHARHDLGIDAGIMVTASHNPPEYA
                     GFKPILGHLPITPGQIDDLRHRMGHRPAPGMVSGGLRMHDALPSYEESIRQMARGLLS
                     ESAAGLRVVVDCGSGCFGPTAPPVLRALGFDVVERFTEPDGTFPHRPSNVALREALEP
                     CIGAVRETGSAFGVAYDGDGDRIAVIDDQGSPVPNDVLVALLAGDALDRHPGGAVVYD
                     VKCSAIVREAVQRAGGVPVVQKSGHTYIKTAMIERHAAFGGEASGHLFYHELHGGDDA
                     LYSTLRVAALVLRSGKPLSALSAAVPRYATTPDLRIGCEGDRGAVIRAIREALRAQTG
                     VRLSDVDGVRADFAEGWGLARVSVTEPVITLRFEAREPGDLRPLMERFLAGAPAGLRQ
                     AVFEATAVGDGCQPDEGGRNDAAHR"
     gene            1964..>2370
                     /locus_tag="PLQ54_13000"
     CDS             1964..>2370
                     /locus_tag="PLQ54_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOS94229.1"
                     /translation="MTDETAAGVGPGIPWEFVGESPRPVERLTLWYRRPAHAARQTGA
                     GPDAPGDDAWVDALPVGNGRLGAMVFGGIGHERIQLNEDSMWAGSPQDADNPEAERAL
                     PEIRRLLQAGDQRAAEERAGRSLVCKGPGTGFG"
BASE COUNT          336 a          781 c          846 g          407 t
ORIGIN      
        1 gaaggattcg tgctcgtcca ggcgcgttcc gatgccgagt ggttcgccgc gcacctggag
       61 ggtgttgcct tggtcgaacg ctggtgtctc gactccctga ccgacctcgg cgcgatggta
      121 ggcggcacga tccgagccac cggaggtggg tcacgaagcg acatctggct gcaagtgcgc
      181 gcccatgtac tccagcgttc gctcgagcgt ccggccgtgg ccgacggtgc catggggtct
      241 gccctccttg cggcatcgcg aacgctgttc gatggcctga ccgccgccgc acgcgccatg
      301 gtccgcatcg cgaagcgcgt cgagccagac gcggccctcg gaccgtgcta tgctgacaag
      361 ctggaccagc tcagggaacg ctgtcgcgag ctgggctatc tctagcctct cgtgggggcc
      421 gtctccttgg atcccttcct tgactgcgat atccggggtg tctacggtca cgagatcacc
      481 gagctcttcg ccagggactt gggtctggct gtcgggtccg tggccgggcg tgggcccgtt
      541 gtcgttgccg gcgactaccg cacccatacg ccggtcctga tggccgcact gtgcgacggg
      601 ctccggcaag ccggggccga cgtggtgtcg tgtgggcaga tacccacccc agtctactac
      661 cacgcgcggc acgacctcgg catcgacgcc ggcatcatgg tgacggcgtc gcacaaccct
      721 cccgagtatg cgggcttcaa gcccatactg ggccacctgc ccatcacgcc aggacagatc
      781 gatgacttgc gccatcgcat gggtcatcgc ccggcccctg ggatggtcag tggtgggctt
      841 cgcatgcacg acgcgctgcc ctcctacgag gagtcgatcc gacagatggc tcgtgggctg
      901 ctctccgagt cggcggcggg attgcgcgtc gtggtcgact gtgggagcgg atgctttggt
      961 cccacggctc caccggtact gcgcgccctg ggcttcgatg tcgtcgagcg cttcaccgag
     1021 cccgacggca cattcccgca ccgaccctcg aatgtggcgt tgcgtgaagc gctcgagccc
     1081 tgcatcggcg cggttcgtga gaccggctcc gcgttcggtg tggcctatga cggcgatggc
     1141 gatcggatcg cggttattga tgatcaaggc agccccgtgc ccaatgacgt gctggtggcc
     1201 ctcctggcgg gtgatgcact cgaccgccat ccgggcggcg cggtggtcta cgatgtgaag
     1261 tgctcggcca tcgtgcgtga agccgtgcag cgggccggtg gcgtacccgt cgtccagaag
     1321 tcggggcaca cctacatcaa gacagcgatg atcgagcgcc atgccgcatt cggcggcgag
     1381 gccagtgggc acctgttcta ccacgagctc cacgggggtg acgatgcgct gtactcgacg
     1441 ctccgcgtcg ccgccctggt gcttcgatcg gggaagccgc tctcagcgct ctcggcggcc
     1501 gtgccgcgct atgcgacgac gcccgacttg cgaatcggct gcgagggcga tcgaggtgcc
     1561 gtcatccgcg cgatccgaga ggccctcagg gcccagaccg gcgtgcggct cagcgacgtg
     1621 gatggcgttc gagctgactt cgccgagggc tggggattgg cccgcgtctc ggtcacggag
     1681 ccggtcatca cactgcgctt cgaggcgcga gagcccggtg acctgcgacc gctcatggag
     1741 cgcttcctcg ctggtgcgcc ggcgggtctg cgccaggcgg tcttcgaggc gacggccgtg
     1801 ggcgacgggt gtcagccgga cgagggaggg cgcaacgatg cggcgcaccg ctgattcccc
     1861 ccgtgtcatg atcctgttgt tggcgcccgt agtggcgtgg ctctcgggcc cattgggcca
     1921 ctcggcatgg ggcgccgacg cccgcacgag agtaggcgat tggatgacag acgagaccgc
     1981 cgcaggcgtt ggtcccggga tcccgtggga attcgtgggt gagtcgccgc ggccggttga
     2041 gcggctgact ctgtggtacc gacgtcccgc ccacgccgcc aggcagacgg gagccggccc
     2101 ggacgcgccg ggcgacgacg cgtgggttga cgccctgccc gtgggcaacg gacgcctcgg
     2161 cgccatggtg tttggcggca tcggccacga gcgcatccag ctcaacgagg actcgatgtg
     2221 ggctggtagc cctcaggacg ccgacaatcc cgaggccgag agggcgctgc ccgagatccg
     2281 acgactgctg caggccggcg accagcgtgc cgccgaagag cgggccggtc gatccctggt
     2341 gtgcaagggg ccgggcaccg gtttcggaag
//
LOCUS       DAOSPL010000733         2362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45100_length_2362_cov_3.629822, whole genome shotgun sequence.
ACCESSION   DAOSPL010000733 DAOSPL010000000
VERSION     DAOSPL010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45100_length_2362_cov_3.629822"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PLQ54_13005"
     CDS             <1..732
                     /locus_tag="PLQ54_13005"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOS94230.1"
                     /translation="LLVRRIEEMKASPPDVVVVSVLGPEEVHDAIVGLKGAFSRIVEG
                     LHLIDEAKRGDRLARPLLIVNTPIIMENAEVLPSLVSLGRRWPILANHFQHLWFTTPE
                     MRAAQCEMDACPDGVLSEGIALETESVDPAHIWRGIRQARAESKGHPVLFYPDLSRRQ
                     VETYYRRPTELVTRDRAICAWIFSHVLPSGEVSPCLGVDAGNLHQDSFLRVWNSDRLR
                     DFRRVLRERRALPICARCCVFFRND"
     gene            776..1657
                     /locus_tag="PLQ54_13010"
     CDS             776..1657
                     /locus_tag="PLQ54_13010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HOS94231.1"
                     /translation="MARSVLIANLGARRATAGRLAALVDVFRSHGELLEVCTSGSPEE
                     ARAMAAAAVATDAPRVWVAGGDGSIHSVLPALTHASTALGVIPWGTGNILARELGIPL
                     NWRRAVRALLRGTVRTLDAGRANGRVFCLFAGVGFDAEVVRNVSPQLKKISGRTAFIA
                     SVCVTTGRWEPFHVKLTLDGEVCDQWAWLVVVANTSAYTGVVNLPDRVRPDDGVLDTV
                     IVRAAPKSRIIWHTAAYALLRRRLAEASRHRAREVRVETDPPVCVQVDGELCEWTPLS
                     VAVDAGALRVVVPASGE"
     gene            1665..1955
                     /locus_tag="PLQ54_13015"
     CDS             1665..1955
                     /locus_tag="PLQ54_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94232.1"
                     /translation="MRFQNWSVIIAMVVMVLLAASTYYRSSAQAAAAPVGRALGFEVI
                     QGQDDERFFAVLMADPEGALRVDVYREDGTLAGQVGLTQEQTTSAPVRILPE"
     gene            2082..>2362
                     /locus_tag="PLQ54_13020"
     CDS             2082..>2362
                     /locus_tag="PLQ54_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94233.1"
                     /translation="MGTLLPILQHQEVLFPGASRSLQICEPRQLALVADCVTWDAPAG
                     VVLACPSDPQLESYATGTLARFDGVTLCGGGHVRAEARGRSPAPQSLAAA"
BASE COUNT          337 a          766 c          841 g          418 t
ORIGIN      
        1 ctactggtgc gtcgaatcga agaaatgaag gccagcccgc ccgacgtggt cgtggtctcg
       61 gtgctggggc ccgaggaggt tcatgacgcc attgtcggtc tgaagggagc cttctcgcgg
      121 atcgtcgagg ggctgcacct gatcgatgaa gccaagaggg gcgaccgact ggcccggccc
      181 ctcctgatcg tcaacacacc catcatcatg gagaacgccg aggtgctgcc atcactggtg
      241 tccctcggcc ggaggtggcc gatcctggcc aaccacttcc agcacctgtg gttcacgaca
      301 cccgagatgc gagccgcgca gtgcgagatg gatgcatgcc ccgacggcgt gctctccgag
      361 gggatcgcgc tggagacgga gagtgttgac ccggcccaca tctggcgcgg aatccgccaa
      421 gcccgggcgg agtccaaggg acatcccgtg ctcttctacc ctgacctatc gcggcggcag
      481 gtcgagacgt actaccggcg cccgacggag ctcgtcacgc gcgatcgcgc gatctgtgcg
      541 tggatcttca gccatgtgct gccgagcggc gaggtgtcgc cctgtctggg agtggacgcc
      601 ggaaacctcc accaggactc attcctgcgg gtgtggaact cggatcgtct tcgcgacttc
      661 cggcgggtgt tgcgggagcg tcgcgctctg ccgatctgcg cccggtgctg tgtgttcttc
      721 cgcaacgact aggcggccgg gcgcccgccg aacgccgagg gggtgtcggg cacccgtggc
      781 gcgctccgtg ctgatcgcta acctcggcgc ccgccgtgcg acggccggga ggctggcggc
      841 gctcgtcgac gtgttccgct cgcacggcga actgcttgag gtgtgcacga gcggcagccc
      901 cgaggaagcc cgcgcgatgg cggccgccgc cgtggccacc gatgcgccgc gcgtctgggt
      961 cgcgggcggg gacgggtcca tccactccgt gcttcccgcg ctgacgcatg cctccacggc
     1021 tctcggtgtc atcccctggg gcacaggcaa tatcctggct cgggagctgg ggattccgct
     1081 gaactggcgc cgagccgtgc gggccctgtt gcgtggcacc gtgcgtaccc tggacgccgg
     1141 ccgcgcgaac ggacgcgtct tctgcctctt cgccggtgtg ggtttcgacg ccgaagtggt
     1201 ccgaaacgta tcgcctcagc tcaagaagat ctcgggccgg acggcgttca tcgcgagtgt
     1261 ctgcgtgacg accgggcgct gggaaccgtt tcacgtgaag ctgacgctcg acggcgaggt
     1321 atgcgatcag tgggcatggc ttgtcgttgt ggccaacacg tcggcgtaca cgggcgtggt
     1381 gaacctcccc gaccgtgtgc gtcccgacga tggtgtactg gacaccgtga ttgtccgcgc
     1441 ggcgcccaag tcccgtatca tctggcacac ggccgcctac gcgctactca ggcgtcggct
     1501 cgcggaggcc agccggcacc gtgcccggga ggtgcgggtg gagactgacc cgccggtctg
     1561 cgttcaggtg gatggcgagc tatgcgagtg gacgccgctc agtgtggcgg tcgacgccgg
     1621 ggcccttcgg gtcgtcgtgc cggcgagtgg ggagtgatgg atctatgcga ttccagaact
     1681 ggtcagtcat cattgccatg gtcgtcatgg tgctgctggc cgcaagcacc tactatcgga
     1741 gctccgctca agcagcggcg gcacccgtcg gccgcgcctt gggcttcgag gtcatccagg
     1801 gacaggacga cgagcggttc ttcgcggtgc tcatggccga ccccgagggc gcacttcggg
     1861 tggatgtcta ccgggaggac ggcacgctcg ccggacaggt cggtctcacc caggaacaga
     1921 ccacctcggc tccggtccgc atcttgcccg aatagccctg cggcctggga cgtcttggga
     1981 gcattccctc gccgatgcac ctggctgcac gccgcgcatg gcataactct ctcgggtccc
     2041 atgtgtcggg gtgcggaggt ttcggtgagg gggcggggcg gatggggacc ttgctgccca
     2101 ttctgcagca ccaggaggtg ctgttcccgg gtgcgtcgcg gtcacttcag atttgtgaac
     2161 cgaggcagct cgccctggtc gcggactgtg tgacctggga cgcgccggct ggcgtggtcc
     2221 tggcgtgccc atccgatcca cagctcgagt cctatgccac cggcacgttg gcccgattcg
     2281 acggggtcac gttgtgcggc gggggccatg tgcgggccga ggcccggggc cgaagcccgg
     2341 cgccccaaag cctggctgca gc
//
LOCUS       DAOSPL010000734         2361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45135_length_2361_cov_2.010841, whole genome shotgun sequence.
ACCESSION   DAOSPL010000734 DAOSPL010000000
VERSION     DAOSPL010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45135_length_2361_cov_2.010841"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1216)
                     /locus_tag="PLQ54_13025"
     CDS             complement(32..1216)
                     /locus_tag="PLQ54_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94234.1"
                     /translation="MLRSKSQILWGYLPGSVFRHEDRVYVRVVTVDGDRLTGLNEAVI
                     FDAIATYLEHWSEDNRYELPLPRDGVVREYRIVRPDQVRWELFPLVLECSRRACARIR
                     SFRSAADLAKSPRCRHCGGQMQQLRFYNAHNCGQISPIHVPKCSTHGYDDLFFENTGS
                     FMTATWRCHGSGCNGGVISRTNMSPCNCGKWAGGDGVVRMRAHTLDDSRPYRAHYLDL
                     INIDSSVFQTYQRHPARSQVAVAHYLELIPTLQDGMREVDTGLDGKRMSQEAWTDKER
                     AYRQMGLDEADIASLRATNGPVESGVAALGDLSTQVLDVVANRRPFYERAAVYDTTEV
                     PRVTLAHQVAQAIASHDSARAAALSSAVELAASMGIREVAVTWDFPVAKVAFGGSSQS
                     RV"
     gene            complement(1216..>2361)
                     /locus_tag="PLQ54_13030"
     CDS             complement(1216..>2361)
                     /locus_tag="PLQ54_13030"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOS94235.1"
                     /translation="LPHYRRKADVAAIIQAELLSAVTEMEDADDPLAELGLDAGLWQE
                     SPTQRDAAELLFDYEVALGYVNSKAHGAKLDEELRLLSDALESDGHGPLDRVVLTGQV
                     PIPELAAAIGRVQEDTLATNRGSRLRALVGTSVVSHGVDLDRLNMLVLAGMPTTAADY
                     IQVTARAGRIHAGLVVTVYDAFSRRERSLFSNFHSYHTFLEQMVTPVPVNKYAFFVAD
                     RTLPGIALALLHDLAREQGLSAPVEGVRLAKHFQTWWNTHRAAIDGRMRDRIQRCYAT
                     LVPGVNDVGMEAELAARALARWDDTESKALSIPARERLTTALFHYQPLANFRDIDDPG
                     EFTVWFHSREAFLRLIGQSVDTEPSPTEEDVAGHGVAQGASPSRESR"
BASE COUNT          428 a          731 c          776 g          426 t
ORIGIN      
        1 actcctcgag accggaggct tcagacgcca actacaccct cgattgtgaa gagccgccaa
       61 aggccacctt ggcaacagga aagtcccagg tgaccgccac ttcgcggatc cccatggagg
      121 ccgcaagttc gactgcagag ctcaaggcgg cggctctagc gctgtcatgc gacgcaatag
      181 cctgcgcgac ctggtgcgcg agggtcaccc gtggaacctc cgtggtgtca tacacggctg
      241 cgcgttcata gaagggacgt cggttggcga caacatctag gacctgcgtc gacaggtcac
      301 ccaaggccgc taccccactc tccaccggcc cgttggtcgc ccgaagggaa gcgatatccg
      361 cctcgtcgag gcccatttgg cgatacgccc gctccttgtc tgtccacgcc tcctggctca
      421 ttcgcttgcc gtcgagaccg gtgtctacct cacgcatgcc gtcttgcagc gttgggatca
      481 gctcgaggta gtgtgcgacc gcgacctggc tgcgcgctgg gtgccgctgg taggtctgga
      541 agaccgatga atcgatgttg atcagatcga ggtagtgagc ccggtagggc cgggagtcgt
      601 cgagtgtgtg cgccctcatg cggaccactc cgtcgcctcc ggcccacttc ccacagttac
      661 agggggacat gttcgttcgg gagattacgc cgccattgca cccggagccg tgacagcgcc
      721 aggtcgcggt catgaagctc ccggtgttct cgaagaacaa atcgtcatag ccgtgagtgc
      781 tgcacttggg aacgtggatg gggctgatct ggccgcagtt gtgcgcgttg tagaagcgga
      841 gctgctgcat ttgccctcca cagtggcggc agcgggggga cttggcgagg tcggcagcac
      901 ttcggaagct gcggatccgg gcacaggcgc gccggctgca ctccaagaca agagggaaca
      961 gttcccagcg gacctggtcg ggccggacga tgcggtattc ccgaacaact ccatctcggg
     1021 gaagcggcag ctcgtaccgg ttgtcctccg accaatgctc gaggtaggtg gcgatggcat
     1081 cgaagatcac ggcctcattg agcccagtga gacgatcgcc gtcgacggta acgacgcgca
     1141 cgtagacgcg atcttcgtgc cgaaataccg agcctggaag atagccccac aggatctgac
     1201 tcttgctgcg cagcatcagc gcgactccct cgatgggctg gcgccctggg cgacgccgtg
     1261 accggccaca tcctcctctg tcggagaagg ttccgtgtcg accgactggc cgatcagtcg
     1321 aaggaatgcc tcgcggctgt ggaaccagac cgtgaactcg ccgggatcat cgatgtcgcg
     1381 gaagttcgcg agtggctgat agtgaaacag agcggtggta agacgctccc gtgcgggaat
     1441 gctgagcgcc ttggactccg tgtcgtccca tcgcgctagg gcgcgggcag cgagctcggc
     1501 ctccatgccc acgtcgttca cccctggaac gagggtggca tagcagcgct gaatacggtc
     1561 gcgcatgcgc ccatcgatgg cggccctgtg ggtgttccac caggtctgga agtgcttggc
     1621 cagccggacg ccttcgacgg gggcgctgag accctgctcg cgggcaaggt catggagcaa
     1681 tgccagagcg atgccgggca gggtccggtc ggcgacgaag aaggcatact tattgaccgg
     1741 caccggcgtc accatctgct cgaggaaggt gtggtagctg tggaagttgc taaacagcga
     1801 ccgttcccga cgggagaacg cgtcgtagac ggtgaccacc agccctgcgt ggatgcggcc
     1861 agcgcgggcc gtgacctgaa tgtagtcagc ggctgtggtc ggcatccccg ccaaaacgag
     1921 catgttgagt cggtcgaggt ccacgccgtg gctgaccact gaggtgccga ccagcgctcg
     1981 caaccggctg ccacggttcg tggcgagggt atcttcctgg acccggccga tggctgcggc
     2041 cagctccgga atcgggacct gcccggtgag tacgacccgg tccagcggtc cgtgcccgtc
     2101 gctctcgagc gcgtcggaca gtagtcgtag ctcctcatca agcttcgcac cgtgtgcctt
     2161 gctgttcaca tagcccagag caacctcgta gtcgaacaag agctctgcgg cgtctcgttg
     2221 agtcggagac tcctgccaga ggccggcgtc caagccgagc tccgcgagtg ggtcgtcggc
     2281 gtcctccatc tctgtcacgg cggaaagcaa ctccgcttgg ataatggccg ccacgtcagc
     2341 ttttcggcga tagtgaggca g
//
LOCUS       DAOSPL010000735         2354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45297_length_2354_cov_3.414093, whole genome shotgun sequence.
ACCESSION   DAOSPL010000735 DAOSPL010000000
VERSION     DAOSPL010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45297_length_2354_cov_3.414093"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="PLQ54_13035"
     CDS             <1..1138
                     /locus_tag="PLQ54_13035"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOS94236.1"
                     /translation="RRFAPELYVSVVHGTAEDRAWQWDAPAHAFITTYETLRSDFTES
                     PRHRVGRTWDLVILDEAQAIKNRESLTSEVCKRLRRRRAWALTGTPLENDVDDLASVL
                     EFVRPLLPGEPQPDLRPGEETIRRQREVQLRRRKADVLPDLPPKIVTTVPLVLGPEQR
                     RAYERAEREGIFELRQLEGELRIGHVFELIMRLKQTCNFCPVTGESAKLEDLAERVAT
                     LAAEGHKAVVFSQFTDQRFGCGALRDGLADLDPLVYTGAMDGPERDRVIRQFTTDAAR
                     PLLIVSLRAGGLGLNLQEASYVFHFDRWWNPAVEHQAEDRVHRVGQALPVHIYQYTCE
                     RTIEERIEQILLGKQTLFQEYVDDVTLDLSQALTREELFGLFGI"
     gene            complement(1142..1546)
                     /locus_tag="PLQ54_13040"
     CDS             complement(1142..1546)
                     /locus_tag="PLQ54_13040"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOS94237.1"
                     /translation="MRASGVRRAMDEGIVSPQDLIEFIRLDMLDGVAMKPARCGGLHS
                     ARRQIEILRDAGLMVLGSGLTDPDLSLAASLILFGAYGITHPCALNGPQFLGDSIATH
                     PLVPVGDETIAVPEGPGLGVEIDEGKLAALAA"
     gene            complement(1589..>2354)
                     /locus_tag="PLQ54_13045"
     CDS             complement(1589..>2354)
                     /locus_tag="PLQ54_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94238.1"
                     /translation="AARARENLEAGKSMHMKMSLKIEVPVAQVPDIDIEIWRKNDKIR
                     AEMPTMNQVSIVAKDEVFSYQGQAGVMLHVPPETMQQFADQRDAVLEKLGLPKDQDEA
                     FVTLLEKDMATIVGEKEYDDIPCYVLQVKPEYCQAFTESLGMAGAMNMTGGLTNVQFG
                     VLQMALEQETGNLRRLYMEMSLMPPGMTVNVDLKLTVTVLEYELNADIPDAMFEFEAP
                     EGTKIIEFTPDRTIEDVQKELLDAAMAQQQAAAPEL"
BASE COUNT          390 a          804 c          737 g          423 t
ORIGIN      
        1 ccgccgcttc gcgcccgagc tctacgtctc ggtcgtccac ggtacggctg aagaccgggc
       61 atggcagtgg gacgctccgg cccatgcctt catcaccacc tatgagacac tccgctccga
      121 cttcaccgag agccccaggc accgcgtcgg ccgtacctgg gatctggtca tcctcgatga
      181 ggcacaggcc atcaagaacc gcgagagcct gaccagcgag gtgtgcaagc ggctccgtcg
      241 gcgccgcgcg tgggcgctca cgggcacgcc gctcgagaac gacgtcgacg atctggcctc
      301 ggtgctcgag ttcgtgcgcc ccctcctgcc gggcgagccc cagccggacc tccggcccgg
      361 agaggagacg atccgccgac agcgcgaagt gcagctccga aggcggaagg ccgacgtgct
      421 gcctgacctg ccgcccaaga tcgtgacgac ggtgcccctc gtgctgggac ccgagcagcg
      481 gcgggcctac gagcgggccg agcgcgaggg catctttgag ctgcgccagt tggaggggga
      541 gctccgcatc gggcatgttt tcgagctgat catgcggctg aagcagacct gcaacttctg
      601 ccccgtgact ggtgaatcgg ctaagctcga ggacctggcc gagcgcgtgg cgactctggc
      661 cgccgagggt cacaaggctg tggtgttctc tcagttcacc gaccaacgct tcggctgcgg
      721 tgcactccgc gacgggctgg ccgatctcga cccactggtc tatacgggcg ccatggacgg
      781 gcccgaacgt gaccgtgtca tccgtcagtt cacgaccgat gcggctcgcc cgctgttgat
      841 cgtgtccctc cgcgccggcg gcctcggact caatctacag gaggcctcct atgtgttcca
      901 cttcgaccgc tggtggaacc cggccgtcga acaccaggcc gaggaccgcg tgcatcgcgt
      961 ggggcaagca cttccggtcc acatctatca gtacacgtgc gagcggacca tcgaggaacg
     1021 catcgagcag atcctgctcg gtaagcagac gctgttccag gagtacgtcg acgacgtgac
     1081 cctcgacctc tctcaagcgc tcacgcgcga ggagctattc gggctgttcg ggatctgaga
     1141 ctcatgccgc caacgccgcg agtttcccct cgtcgatctc gacacccagc ccagggccct
     1201 cgggcacggc gatggtttcg tcgccgactg gcacgagtgg gtgcgtggcg atcgaatcgc
     1261 cgaggaactg cgggccgttc agggcgcacg ggtgcgtgat gccgtaggcg ccgaacagga
     1321 tcaggctggc cgccagcgac agatcgggat cggtgaggcc gctgccgagg accatgagcc
     1381 ccgcatcgcg caggatctcg atctgccgcc gggccgagtg caggccgccg cagcgcgccg
     1441 gcttcatggc cacgccgtcg agcatgtcca gccggatgaa ctcgatcagg tcctgcgggg
     1501 agacgatgcc ctcgtccatc gcgcgccgaa cgcccgaggc gcgcagcaga acgccacgcg
     1561 cctcaggggt tgtctcacat cccgtcgcct acaactcagg cgcggccgcc tgctgctgtg
     1621 ccatggccgc gtccagcagc tccttctgga cgtcctcgat cgtacgatct ggcgtaaact
     1681 cgataatctt ggtcccctcg ggcgcctcga actcgaacat cgcgtcgggg atatcggcgt
     1741 tgagctcgta ctcgagcacc gtaacggtga gcttcagatc cacgttgaca gtcatccccg
     1801 gtggcatcaa ggacatctcc atgtacagcc ggcgcaggtt gcccgtctcc tgctcgaggg
     1861 ccatctgcag cacaccgaac tgcacattgg tgaggccacc agtcatgttc atggcgcccg
     1921 ccatacccag tgactcggtg aacgcttggc agtactcggg cttcacctgg agcacgtagc
     1981 aggggatgtc gtcatactcc ttctctccga caatcgtcgc catgtccttc tcgagcagag
     2041 tcacgaaggc ctcatcctga tccttcggca ggcccagctt ctccaggacc gcgtcgcgct
     2101 gatcggcgaa ctgctgcatg gtctccggcg gcacgtggag cattacgccc gcctggccct
     2161 ggtaggagaa gacctcgtcc ttagccacga tgctgacctg gttcatggtc ggcatctcgg
     2221 cccggatctt gtcgttcttc ctccagatct cgatgtcgat gtccggaacc tgcgcgaccg
     2281 gtacctcgat cttgaggctc atcttcatgt gcatcgactt gccggcttcc aggttctctc
     2341 gcgctcgggc ggcc
//
LOCUS       DAOSPL010000736         2354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45299_length_2354_cov_3.092214, whole genome shotgun sequence.
ACCESSION   DAOSPL010000736 DAOSPL010000000
VERSION     DAOSPL010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45299_length_2354_cov_3.092214"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PLQ54_13050"
     CDS             <1..747
                     /locus_tag="PLQ54_13050"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOS94239.1"
                     /translation="VMTSIDQQAECAARMRQLSIALGRYMDEHGQRLPRAEEWVTAAK
                     AYLQNPAAVSCPSAAGDVPSIAMNGQLSGLRGVDIAAPERTVLLFDSQPGVSVGTDES
                     AMARRHNHLATVLLASGTVVTVDSTATLKWTPGATRPATPSGGVTRPTAIRNAVEYVV
                     LRDIVEPLGGRVSWNAQTKTITAMLGGRTLDVTDGQSTAMLDGAPLPLAAPPVTIDGK
                     LLVPASTVSRAFRLWMRWSDGQVTVSLGQD"
     gene            790..1179
                     /locus_tag="PLQ54_13055"
     CDS             790..1179
                     /locus_tag="PLQ54_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOS94240.1"
                     /translation="MAHHEALTSPAAAPPIGPYSHAVRCGDLLFVSGQLPADPATGAL
                     VEGGAGAQAARVLENLRVLLESLDSGLPHVLKTTVFLTDMDDFAEVNEVYARYFSQAP
                     PARSCVAVAALPKGARVEMDCIAALGE"
     gene            1186..1956
                     /locus_tag="PLQ54_13060"
     CDS             1186..1956
                     /locus_tag="PLQ54_13060"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019550736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOS94241.1"
                     /translation="MPETGVRLNKLLARRGVGSRAAVERLIADGHVTVDGEPVTIQGF
                     RLWDRAVVEIDGRPLPPPCGFRYVAFHKPPGYVTTRRDVSGKPTICDILPPELEMLHP
                     VGRLDRDSEGLLILTTDGELTLHLTHPGHEVEKVYHALVRGVPSEAAIRCLSEGLDLR
                     DGPTRPARAEVLSPAEDGCTWVLIGLREGRRRQVRRMLRAVGHLVQRLVRVSIGPVAL
                     GDLAPGAWRDLMPEEVRALRRCGCTPTGPTRPRFRVQT"
     gene            2120..>2354
                     /locus_tag="PLQ54_13065"
     CDS             2120..>2354
                     /locus_tag="PLQ54_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94242.1"
                     /translation="MRTLLLLLVAAVVVVGLVALTGCPPKDDGGAGEPIVTKPVNAGK
                     APGGPAKVEEKTGPAETEDAAKTEEPAGDEAKTG"
BASE COUNT          381 a          770 c          826 g          377 t
ORIGIN      
        1 gtgatgacat ccatcgacca acaggctgag tgtgctgcgc gcatgaggca gctctccatc
       61 gccctgggcc ggtatatgga tgaacacggc cagcgtctgc ccagggcgga ggaatgggtg
      121 accgccgcca aggcgtatct ccagaacccg gctgccgtca gctgtccgtc ggcggccggc
      181 gacgtaccgt ccatcgccat gaacggacag ctatccggcc tgcgaggagt ggacattgcg
      241 gccccggaga ggaccgtgct gctattcgac tcgcagcctg gagtgtccgt ggggaccgac
      301 gaatccgcca tggccagacg tcacaaccac ctggcgacgg tgctcctcgc cagcggcacc
      361 gtggtgacgg tcgacagcac ggccacgctc aagtggacgc ccggcgcaac gcggccggcg
      421 actccttcgg gcggcgtcac ccgccccact gctatccgga acgcggtcga gtacgtggtg
      481 ttgcgcgaca tcgtggagcc cctcggcggc cgggtgtcat ggaacgcgca gaccaagacc
      541 atcaccgcga tgctcggggg caggaccctc gacgtgaccg acgggcagtc gaccgcgatg
      601 ctggatggcg ccccgttgcc gctcgcggcg ccgccggtca ccatcgacgg aaagctcttg
      661 gtaccggcca gcacggtgag ccgggcattc agactgtgga tgcgatggtc ggacggacag
      721 gtcacggtct ccctcggcca agactagcca taaccggccg tatccgcctc tgttgggagc
      781 gatgccagca tggcgcatca tgaagcactg accagccccg ccgccgcccc gcccattggc
      841 ccctattccc acgccgttcg gtgtggcgac ctgctgttcg tgtccgggca gctgccggct
      901 gacccggcta ccggggcact ggtggagggc ggggcaggcg cccaggccgc ccgcgtgctc
      961 gagaacctcc gggtcctcct cgaatccctg gactcgggct tgccgcacgt gctcaagacc
     1021 accgtgtttc tcaccgacat ggacgacttc gctgaggtca atgaggtgta cgcccgctac
     1081 ttctcgcagg cgcccccggc gcggagctgt gtggcggttg cggcactgcc gaagggtgcc
     1141 cgggtcgaga tggactgtat cgccgccttg ggcgagtgag gcggattgcc cgagacaggc
     1201 gtgcgcctca acaagctgct ggcccgccgg ggagtcgggt ctcgagcggc ggttgaacgt
     1261 ctcattgccg acgggcatgt caccgttgat ggtgagccgg tcacaatcca ggggttccgc
     1321 ctctgggatc gtgcggtggt cgagatcgac ggtcggccac tgcctccccc atgcggtttc
     1381 cgctacgtgg ctttccacaa gcctccgggt tacgtgacga cacggcgcga cgtgtcgggg
     1441 aagcctacca tctgcgatat cctgccacca gagctagaga tgctccatcc cgtgggccgg
     1501 ctggaccggg actcggaggg actcctgatc ctgacgactg acggcgaact cactctgcac
     1561 ttgacgcacc cagggcacga agtggagaag gtctaccacg cgctggtgcg cggcgtgccc
     1621 tccgaggccg cgatccgctg cctgtccgag ggtcttgatc tgcgcgatgg gccgacgcgg
     1681 cccgcccggg cggaggtgct gagccccgcg gaggacggct gcacgtgggt cctcatcggc
     1741 ctcagagagg ggcgcagaag gcaggtgcgc cggatgctga gggccgtggg ccacttggtg
     1801 cagcggctgg tccgagtgag catcgggccg gtggcgctcg gggatctggc gccgggcgcg
     1861 tggcgcgacc tgatgccgga ggaagttcgc gccttgcgac gatgcggctg cacaccgacc
     1921 gggccgactc gcccgcgatt tcgcgtccag acttagaggc gaagggctaa ctcatagacg
     1981 ggtcgaatcc catagaggcc tgtggtctgc cgacgcgagc catacgcgca ctcttgacgc
     2041 aggccatagg gagctccggt cgtcgccgcc acgcgcagac cgggccccag aggctcacca
     2101 tgtgtaggag gtcgtagcgt tgaggactct tcttctgctt ctggttgctg cagtggtagt
     2161 tgtgggccta gtggccttga cgggctgccc acccaaggat gacggtggcg ccggggagcc
     2221 cattgtgacc aagcccgtga atgccggtaa ggcgcctggc ggtcccgcca aagtcgagga
     2281 gaagacgggc ccggcagaga ccgaggatgc ggccaagacc gaggagcccg ccggcgacga
     2341 agccaagacc ggcg
//
LOCUS       DAOSPL010000737         2351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45378_length_2351_cov_3.215157, whole genome shotgun sequence.
ACCESSION   DAOSPL010000737 DAOSPL010000000
VERSION     DAOSPL010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45378_length_2351_cov_3.215157"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /locus_tag="PLQ54_13070"
     CDS             complement(<1..1457)
                     /locus_tag="PLQ54_13070"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOS94243.1"
                     /translation="MTPVFGVVAALVAAAPPDGLALYCPFDGSLEPLWCGEGMTRMPA
                     DLPLVPGMRGSAVHLVGDYAFSVDGAFPVAEGTFGLWVALDAIEDVDAPRYLMCIYGH
                     PSVAESWMHNRFCLFVQGGRLTMQVFPREGAVASAQADVPAWQPGEWHHVAATWSGIN
                     SGQPRAALRLFADGEVVGESAGLQLDVGPTSTVWHVGRDSDNSADYGWAAFDDLFLYG
                     RALPPDEIASGVAWVKAHTGATARVPAARQVPGWLAPELPFRATALVVPARRDRTDPV
                     AHLPMGTGGTLGVPGGLEQLLPSVCRVFAEGTELAAEPAAGVVRWVVPGRIAAGEERH
                     FDVYFGAARYAYAGPLQVTCEMPTTSASETALAPTDFATATFGDAWDFDEGDIEDIDQ
                     FGDKPEYIRNVRVEDGALQADVEQDAYIVWGSMWGPEDQGKRTFRLDPTEYNQLEIRL
                     RQSVPWSQWDIMGRVAGTDGLAVYKFPVSGQGWQT"
     gene            complement(1519..2154)
                     /locus_tag="PLQ54_13075"
     CDS             complement(1519..2154)
                     /locus_tag="PLQ54_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94244.1"
                     /translation="MQRHRRPFGLLVVVAIATGWVLWTGTPRARAGIFDDIADEQVDP
                     DKMVEDTAMAEHAGFLYLARGDSLYQCDAETLGTLREIRLRDVRKLGTAAVGVIDGFL
                     YVVKDDRVFKFDPEHLDLVAMADMDHSAGRAHPGMVADGDSLYVAVRDFAYRFRLSDL
                     QELGRAELKHSPERGACAASLAGRWLYVAKETRIYKIDTESMTRVGQSKFD"
BASE COUNT          397 a          852 c          751 g          351 t
ORIGIN      
        1 atcgtctgcc agccctggcc gctgaccggg aacttgtaca cggcgaggcc gtcggtgccc
       61 gccacccgcc ccatgatgtc ccactgggac catggcacgc tctgccgcag ccggatctcg
      121 agttggttgt actcggtggg atccaggcga aaggtccgct tgccctggtc ctccggcccc
      181 cacatggagc cccaaacgat gtaggcgtct tgctcgacgt cagcctgcag cgcaccgtcc
      241 tcgacgcgca cgttgcggat gtactcgggc ttgtcgccaa actggtcgat atcctcgatg
      301 tcgccctcgt cgaagtccca ggcatcaccg aaggtggccg tggcgaagtc cgtgggcgcg
      361 agggccgtct cactggccga cgtggtaggc atctcacagg tgacttgcag cggaccggca
      421 taggcatagc gggccgcacc gaagtagaca tcgaagtggc gctcctcacc cgccgcgatg
      481 cggcccggca ccacccagcg gaccactccg gccgcaggct ccgccgccag ttccgtcccc
      541 tcggcgaaca cgcggcatac cgagggcagg agttgctcga ggccaccggg gacgcccagt
      601 gtgccaccgg tgcccatcgg gagatgggcc acggggtccg tccggtcccg ccgggcgggc
      661 acgaccagcg ccgtggcgcg aaagggtagc tcgggcgcca gccagccggg cacctgacgg
      721 gcagcaggga cacgcgccgt cgcgcccgtg tgcgccttca cccaggctac gcccgacgcg
      781 atctcatccg gcggcagcgc ccggccgtac aggaacaggt catcgaatgc agcccagccg
      841 tagtcggccg agttgtccga gtcgcgaccc acatgccaca ccgtggaggt gggccccacg
      901 tcgagctgga gaccggcgct ctcgcccaca acctctccgt cggcgaacag ccgcaaggcg
      961 gccctgggct gaccgctgtt gatacccgac cacgtggccg ccacgtggtg ccactcgccc
     1021 ggttgccagg ccggcacatc ggcctgcgcg ctggcgacgg cgccctcccg cgggaagacc
     1081 tgcatggtca gccggccgcc ctggacgaac aggcagaacc ggttgtgcat ccagctctca
     1141 gccaccgacg ggtgtccgta gatgcacatg aggtagcgcg gcgcatcgac atcctcgatg
     1201 gcgtcgagcg caacccacag cccgaaggtg ccttcggcca ccgggaacgc gccgtccaca
     1261 ctgaaggcat agtcgcccac gagatgcacc gcactgccgc gcatgcccgg tacgagcggc
     1321 aggtccgcgg gcatgcgggt catgccctcc ccacaccaca gcggctccag tgaaccgtcg
     1381 aagggacagt agagggcaag accgtcgggc ggcgcggccg ccaccagtgc tgcgaccact
     1441 ccgaagactg gagtcacggc acatcacctt cctggcatcc agggcaccat atggtccagc
     1501 tcgggcccgc cgtaccgctc agtcgaactt gctctggccc acccgggtca tcgattctgt
     1561 gtctatttta taaatacgcg tttccttggc cacgtagagc cagcgccctg cgaggcttgc
     1621 cgcacacgcg ccccgctcgg gcgagtgctt gagctccgcg cgaccgagct cctggagatc
     1681 gctgagccgg aaccggtatg cgaagtcccg aacggccaca tagagcgaat ccccgtcagc
     1741 caccatcccc ggatgggcgc gtcccgcgct gtggtccatg tcggccatgg ccaccaggtc
     1801 caggtgctcc gggtcgaact tgaacacgcg gtcatccttg accacataga ggaacccatc
     1861 gatcaccccg acagcggccg tccccagctt ccgcacatcg cggagccgga tctcgcgcag
     1921 cgtccccagc gtctccgcat cacactgata gaggctgtcg cctcgcgcca gatagaggaa
     1981 gccagcgtgt tcggccatgg ccgtgtcctc caccatcttg tccgggtcca cctgctcgtc
     2041 agcgatgtcg tcgaagatcc cggcgcgagc cctcggggtc ccggtccaca ggacccaccc
     2101 cgtggcgatg gccaccacca ccagaagccc gaatggtcgt cgatgccgct gcatcggctt
     2161 ccctccgttc cgaatgcgcg tgcgctccgt gcattgtacg acgttgggcc ccgcgaagga
     2221 ggcccgggtc cgcgggccga agcgcggcgg agccgcaacg agccatcccg aacggagtgg
     2281 gtttgcagtg ggtctgatga tcggagtggg aagggcggac atcaccgccc cagtcggcgc
     2341 gcacctgagt g
//
LOCUS       DAOSPL010000738         2350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45399_length_2350_cov_2.987800, whole genome shotgun sequence.
ACCESSION   DAOSPL010000738 DAOSPL010000000
VERSION     DAOSPL010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45399_length_2350_cov_2.987800"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLQ54_13080"
     CDS             <1..211
                     /locus_tag="PLQ54_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94245.1"
                     /translation="GSVFGDAVAAWHMRDGSDGSGNADLRAVGDVALGVALEGEERTA
                     SITRGGDGYAARFDGGYPTQGAMGR"
     gene            208..2064
                     /locus_tag="PLQ54_13085"
     CDS             208..2064
                     /locus_tag="PLQ54_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94246.1"
                     /translation="MNLQGKALTLCLRARDPSGKWDSPLFSKHGGHAVLTYNLFSADL
                     GEGAVVGFELGTDWSARPLRVSVPLTRIDARDWHDFIVRYSGAKLELFVDGVLVDEEW
                     PMGSLRSGNTVPCLVGAEWSGEAVKAGFRGLVDHASLWNRALTDDEIVTLSGGAAHVA
                     LRDRQVLGPERTHLQYWRPRQIGAAVGDCMPFWHDGRFHLFYLFDRRHHGSKWGLGAH
                     QWAHISTTDLIRWEHHPMAIPITDEREGSICTGSVLFHEGTYYAFYATRLPDGSQHLS
                     LATSADGVHFEKQGPAPLASPPEGYDPLHFRDPFALLDEAAGLFRLIVTARLTDGRDG
                     CLAQLTSTDLRHWQMAEPFLILGRVTDCPDVFEWNGWYYLLAEHCYWVSRRLEGPWVQ
                     PDPNRLDVLYVPKTAPFREGRRIYASWLPDQGWGGDLVFRELVQLPDGRLGTCFPDEL
                     IPPSHHAIPGRFTPVTGEPEMGTNRLSLRRGRGFEAATCPVPDDAVITLQIRPGSAAL
                     QYGLVVHTDERMAGGCEIRMTPAAQTVELLCRQPDGGTEPLAQIANVMGLENPFRIEI
                     VTRGDIVDVCIDHRRTIIARCPSRSGARLGAYAQDARVGFESIRVHTIADEP"
BASE COUNT          338 a          794 c          839 g          379 t
ORIGIN      
        1 cggaagcgtc ttcggcgacg ccgtagccgc ctggcatatg cgcgacgggt ccgatgggag
       61 cgggaacgcc gacctgcgcg ccgttggcga tgtggctctc ggcgtggcgc tggagggcga
      121 ggagcggacc gcgtccatca cccgtggcgg ggacggctac gccgcgcggt tcgatggtgg
      181 ctacccgacg cagggtgcga tggggcggtg aaccttcagg gcaaggccct cacgctgtgc
      241 ctccgcgcgc gcgacccgtc ggggaagtgg gactcgccgc tgttctcgaa gcacggcggc
      301 catgcggtgc taacgtacaa cctgttctcg gccgacctgg gcgagggcgc tgtggtgggg
      361 ttcgagcttg ggaccgactg gtcggcgcgg ccgctgcggg tgagtgtgcc tctgacgagg
      421 atcgatgcac gcgactggca cgacttcatc gtgcgctact cgggcgccaa gctggagctg
      481 ttcgtggacg gcgtgctcgt ggacgaggag tggcccatgg gctcgctccg cagtgggaac
      541 accgtgccgt gcctggtcgg ggccgagtgg agtggcgaag cggtgaaggc gggcttccgg
      601 gggctcgtag accacgcgtc tctctggaac cgggcactca ccgacgacga gatcgtgacg
      661 ctcagtggcg gtgcggccca cgtggcgctg cgggacagac aggtcctcgg tcccgagcgc
      721 acccacctgc agtactggcg tccgcggcag atcggggcgg ccgtgggcga ctgcatgccc
      781 ttctggcacg atgggcgctt ccacctgttc tacttgttcg accggcgcca ccacggcagc
      841 aagtggggcc tgggcgccca tcagtgggcg cacatttcga ccaccgatct gatccggtgg
      901 gagcaccacc cgatggcgat cccgatcacc gacgagcggg agggctcgat ctgcacgggg
      961 tcggtcctct tccatgaggg aacctactac gcgttctatg ccacccggct tccggacggc
     1021 agtcagcacc tgagcctggc caccagcgcc gatggggtgc atttcgagaa gcagggcccc
     1081 gcgccactgg catcgccgcc cgaggggtac gacccgctgc acttccgaga tcccttcgcg
     1141 ctcctcgacg aagcggcagg cctgttccgg ctcatcgtga cggcccgcct caccgacggg
     1201 cgtgacggat gtttggcgca gctcacctcc accgatctcc ggcactggca gatggcggag
     1261 ccattcctca tcttgggccg tgtcaccgac tgcccggacg ttttcgagtg gaacggctgg
     1321 tactacctac tggccgagca ttgctactgg gtgtcccgtc gtctcgaggg cccgtgggtc
     1381 cagccggacc cgaaccggct cgatgtgctc tatgtgccca agaccgcccc attccgtgag
     1441 ggccgccgga tctacgcatc gtggcttccg gaccagggct ggggcggcga cctcgtgttc
     1501 cgcgagctgg tccagctccc ggacggccgg ctggggacgt gcttccctga cgagctcatt
     1561 ccccccagtc accacgcgat cccggggcgg ttcaccccgg tcacgggaga accggagatg
     1621 ggcacgaacc ggctgtcgct ccggcggggc agagggttcg aagcggccac ctgtccggtt
     1681 ccggatgacg ccgtgatcac gctccagatc cgtcccggct ccgctgccct ccagtacggg
     1741 ctggttgtgc acacggatga gcggatggct ggtgggtgcg agatccggat gacgccggcg
     1801 gcccagacgg tcgagttgct gtgccgtcag cccgatggag ggacggagcc actggcccag
     1861 atcgccaacg tgatggggct tgagaacccc ttccggatcg agatcgtgac gaggggcgac
     1921 atcgtcgatg tgtgcatcga ccaccgccgc accatcatcg cccgatgtcc cagccgatcg
     1981 ggagctcgac tcggcgccta cgcccaagac gcgcgggtgg ggttcgaatc gattcgcgtg
     2041 cacaccattg ctgacgagcc gtagggggcc accgtgccac ccgagacgac ggggccccgc
     2101 cccgctgcct tgcccgcgcc cggccggccc ttcgtcatgc gctgcagccc ggcgcggctc
     2161 agggcgaacg gacacgctga cacgggcgac cccgggcgcg ctggggcccc tcggcagagt
     2221 gaggttgcgc gtgcggcgcg accttcgcgg gccccagtcc ccggagcggg tgcctccagc
     2281 tggcgcccat gcctatgcgg ctgggaggat gcccttggcc gccgcgtcag cgaacttggc
     2341 cgccgccaga
//
LOCUS       DAOSPL010000739         2348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45446_length_2348_cov_3.044483, whole genome shotgun sequence.
ACCESSION   DAOSPL010000739 DAOSPL010000000
VERSION     DAOSPL010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45446_length_2348_cov_3.044483"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLQ54_13090"
     CDS             <1..449
                     /locus_tag="PLQ54_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS94247.1"
                     /translation="IVQLHCLMDGAWPTAHQREMELLEGAKARGAIRAHGVSIHSLPA
                     LKACVDCEWVDVVHVRLNAYGDRMDDPDPNVVADVVRQLHDAGKGVLAMKLIGEGAYR
                     DDPAKRDESIRFVLSQGTVSSMIVGFEKPEEIDDFAARVQAVLAAK"
     gene            complement(566..850)
                     /locus_tag="PLQ54_13095"
     CDS             complement(566..850)
                     /locus_tag="PLQ54_13095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HOS94248.1"
                     /translation="MVKLVAVLKAKPGQEAAVTQALRDLAVPSKAEPGCVMYVPCRSK
                     ADPAKLVVIEEWADQAAFDAHAASPHFQAFLAKVGNALAGEPELEFVEVL"
     gene            complement(1027..1281)
                     /locus_tag="PLQ54_13100"
     CDS             complement(1027..1281)
                     /locus_tag="PLQ54_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94249.1"
                     /translation="MKYVALVLLLVVLVTLLVATVGCKGEDPGANSPEAKAADAAAQQ
                     KCAGFQPPAERPGGGTGPPPGKQLPAWKTGAGGAAGGTGQ"
     gene            complement(1351..2043)
                     /locus_tag="PLQ54_13105"
     CDS             complement(1351..2043)
                     /locus_tag="PLQ54_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOS94250.1"
                     /translation="MKRRGFTLIELLVVIAIIAILAAILFPVFAKAREKARQASCQSN
                     LKQLGLAMLQYASDNDQMWARAWDPQWGAWGGPVLYCDVIMPYVKNKQVFSCPSRRTG
                     PFWNELTLNSSNQVVSDVGGLHTQCCYGVNCGVLIKNRCKCAAATDSDITRPSEIIAL
                     GDMWTNSTDGRINPMACCGSYCHGTPANVHNEGGNYLYFDGHVKWHKPAQIGYNAASD
                     GWNVARKYWSPW"
BASE COUNT          367 a          817 c          771 g          393 t
ORIGIN      
        1 acatcgtgca gctccactgc ctgatggatg gcgcgtggcc cacagcacac cagagggaga
       61 tggaactgct ggagggcgcg aaggccaggg gcgccatcag agcccacggc gtgtcgatcc
      121 actccctgcc cgccctgaaa gcctgtgtcg actgcgagtg ggttgacgtg gtgcacgtcc
      181 gcctgaacgc ctacggcgac cggatggacg atcccgaccc gaacgtggtc gccgacgtcg
      241 ttcggcagct ccacgatgcg ggcaagggcg tcctggccat gaagctcatc ggcgagggcg
      301 cgtaccgcga cgaccccgct aagcgcgatg agtccatccg gttcgtgctg agtcagggca
      361 cggttagcag catgatcgtc ggctttgaaa agcctgagga gatcgatgac ttcgccgcac
      421 gcgttcaggc ggtgctggcg gccaagtaga cgccacccgc cgtccggtcg cgcaccgcag
      481 ggctccgccc cgcaccccgc tgccgcgccg gaccgcgggg cgctgcctgt cgtccgccgt
      541 cacaccgcgc catggctcgg cccgcctaca gcacctcgac gaactcgagt tccggctccc
      601 cggccagcgc gttgcccacc ttcgcaagga atgcctggaa gtgaggcgag gcggcatggg
      661 cgtcgaatgc ggcctgatcg gcccactcct cgataaccac gagcttggcc gggtcggcct
      721 tcgaccggca gggaacgtac atgacgcatc cgggctcggc cttgctcgga accgcgagat
      781 cgcgcagcgc ctgggtgacc gcagcctcct ggccgggctt cgctttgagg actgcaacga
      841 gcttgaccat gtaggggcct ccatcatggc ccggcaggct tgcggccggg cattgagcga
      901 tggggttcgc tcagggcggg acccggtcct gcctcgaggt ggaccgtacg gcgcgagggg
      961 catcctcccg tcaccatgcg tggcaggaga atgcccccga gcggagcgac ggtgctcgac
     1021 gcgcgcctac tgacccgtgc ctccagcggc ccccccagcc cccgttttcc aggcgggcag
     1081 ttgcttcccg ggtggcggcc cggtgccgcc acccggccgc tcggccggcg gctggaagcc
     1141 tgcgcacttc tgctgagcgg cggcgtccgc cgccttggcc tcggggctgt tggctcccgg
     1201 gtcctcgccc ttgcacccca cggtggccac gagtagcgtc acgagcacaa ccagcagcag
     1261 caccaacgct acgtacttca tggaaccctc ctgattacat ggatgcagcc gtccatgtca
     1321 gccggccggt cctcgcggtg cgccctgttc ctaccagggc gaccaatact tgcgcgcgac
     1381 gttccagcca tcggaggccg cgttgtagcc gatctgcgcc ggcttgtgcc acttgacgtg
     1441 cccgtcgaag tagaggtagt tgccgccctc attgtggaca ttcgcggggg tgccgtggca
     1501 gtagctgccg cagcaagcca tcgggttgat ccgcccatcg gtggagttgg tccacatgtc
     1561 gccgagagcg atgatctcgg aggggcgggt gatgtccgaa tcggttgctg cggcgcactt
     1621 gcagcggttc ttgatcagga ccccgcagtt gaccccgtag cagcactggg tgtggagccc
     1681 accgacatcg gagaccacct ggttcgagct attcagcgtg agttcattcc agaacggccc
     1741 cgtgcgccgg gacgggcacg agaagacctg cttgttcttg acgtagggca taatgacgtc
     1801 gcagtagagg actgggccac cccaggcgcc ccactgcgga tcccaggcgc gggcccacat
     1861 ctggtcgttg tccgatgcgt actgcagcat cgccagcccc aactgcttca ggttgctctg
     1921 gcaggacgcc tgccgcgctt tctctcttgc cttggcgaac acgggaaaca gaatggccgc
     1981 cagaatcgcg atgatcgcaa taacgacgag caattcgatg agcgtgaagc cccttctctt
     2041 catcgtgcat cccctttcgc agactcctct tccccggcga gtgcgccata ccggagtgac
     2101 catagtaagt cgcttcgaca ccaaccgccg atgtcctcct tctgggtcgg aggcgcgtgc
     2161 cagcggagta gccgacgccc gggccgccct ctgccgcccg cccgaggtca gctacgggga
     2221 gtgagccctt cgccccggcc ggcacggcaa taccccccgg gccctgtgcg gcgaagggct
     2281 cgtcccctcg gtgtggtccg tggcccgcgg tctccgcgcc gcggggccgg ccgcaggccg
     2341 acaacgcc
//
LOCUS       DAOSPL010000740         2348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45447_length_2348_cov_2.988661, whole genome shotgun sequence.
ACCESSION   DAOSPL010000740 DAOSPL010000000
VERSION     DAOSPL010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45447_length_2348_cov_2.988661"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1698)
                     /locus_tag="PLQ54_13110"
     CDS             complement(<1..1698)
                     /locus_tag="PLQ54_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94251.1"
                     /translation="MNRPHCLSSMLVRGVGWGALAAVLLGLGALPAYAADPWNATNPE
                     LERFGYDRLVGAGDPHRTTASYRYSGDGSVGVATGRHHWAYGGGAWAQEINGSWPIPT
                     FYWQWDMNNLDPPATTTFILAHNSATDANGRLYLDVDIQRVIPNGTTSFDIARDIGPN
                     SFVLTSGRTYYWQVYTSNADGNNGDVWTFTANQAPNRANLIWPPDPRYGVNPPEGYEG
                     VGVIESYRAYHDNRHGLGEKVVSPYYGPTLLFTATDPDGGPGTAQPGYDYNIPFSSTA
                     LYAQIFLTDKKDPWEDPGSESGAAPNRWEITDFVQWNFAADDVANMAGPFYQPYGHYR
                     PALQSHASRILDLPSDKTPYSNYTQPPFGTWPQVSYLLATPDELIPGLRGWADGVLGN
                     PPMLIVAREPLQTNRDYLWRVRLFDGDLFGEISPTVTVDNNMDMQPDWPAEQTGITQG
                     GFQQPFAFRTVPNNLPEPAYQPNPPDYRLPGPPAASMNVATSPPPTLTWKWDEPDGQP
                     VTFDVYFGTEFNPSNGHSLSAPPLAASNLTQAKYTPSSLRGNKRYFWRVVTRDSQGRM
                     "
     gene            complement(1758..>2348)
                     /locus_tag="PLQ54_13115"
     CDS             complement(1758..>2348)
                     /locus_tag="PLQ54_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94252.1"
                     /translation="GRSIDEGTASKTGRRLEVVALVSTPIVSEIARQAAVRIGHELNA
                     SHDMLRALKIHLYDDAGALQANAPPVAVVTWGPGGQYELPRREGPVNLDDYFFLYEWD
                     EAKWKANEELVAKKHLPGWLPPYEVKPPDKPRAGEATGKSIGQYAVPALMGVGIGAAA
                     LGAGALVVRRRRRRTIDAEPAPRPRQRAGRGSRSKH"
BASE COUNT          361 a          728 c          838 g          421 t
ORIGIN      
        1 catgcgaccc tgagagtcgc gggtgacgac tcgccagaag taccgcttgt tgccccggag
       61 cgacgacggc gtgtatttgg cctgggtgag gtttgatgcc gccagcggtg gcgcggagag
      121 gctgtgtcca ttgctcgggt tgaactcggt tccgaagtac acgtcgaacg tgacgggctg
      181 gccgtccggc tcatcccact tccaggtcag agtcggcggc ggggacgtgg cgacgttcat
      241 ggacgccgcg ggcgggccgg gcaggcggta gtccggcgga ttgggctggt aggcgggttc
      301 cggcaggttg ttcgggaccg tgcggaacgc gaagggctgc tggaagccgc cctgagtgat
      361 gcccgtctgc tccgccggcc agtcgggctg catgtccatg ttgttgtcta ccgtcaccgt
      421 gggggagatc tcgccgaaca ggtcgccatc gaacagccgg acgcgccaca ggtagtcgcg
      481 gttggtctgc agcggttcgc gcgccacgat cagcatgggt gggttaccca ggaccccatc
      541 ggcccagccg cggaggccgg ggatcaactc atcgggggtc gccaacagat acgacacctg
      601 aggccaggtg ccaaaaggcg gctgggtgta gttgctgtaa ggagtcttgt cggaaggcag
      661 gtcgaggatc cgcgaggcat ggctctgcag cgcgggccgg tagtggccgt agggctggta
      721 gaacggtccg gccatgttgg ccacatcgtc cgcggcgaag ttccactgga cgaagtcggt
      781 gatctcccac cggttcgggg cggcgccgga ctctgacccc ggatcttccc acgggtcctt
      841 cttgtcggtc aggaagatct gtgcgtacag ggcggtgctg gagaagggga tgttgtagtc
      901 gtagccgggt tgcgcggtgc caggcccgcc gtcggggtcg gttgccgtga acagcagggt
      961 cggcccgtag tagggcgaga cgaccttctc gccgaggcca tgccggttgt catggtaagc
     1021 gcggtaggac tcgatgacgc ccacaccctc atagccctcg ggcgggttga caccgtaccg
     1081 gggatcgggg ggccagatga ggttggccct gttgggcgcc tggttggcgg tgaacgtcca
     1141 cacgtcgccg ttgttgccgt cggcgttcga ggtatagacc tgccagtagt acgtgcggcc
     1201 ggacgtgagc acgaacgagt tggggccaat gtcccgggcg atgtcgaagc tggtcgtgcc
     1261 gttggggatc actcgctgga tgtcgacatc caggtacaac cggccattgg cgtccgtggc
     1321 gctgttgtgc gcgaggatga aggtggtggt ggcgggcgga tcgaggttgt tcatgtccca
     1381 ttgccagtag aaggtcggaa tgggccatga gccattgatc tcctgtgccc acgcgccgcc
     1441 gccataagcc cagtgatggc ggccggtggc tacgcccacg gagccgtcgc cgctgtagcg
     1501 gtatgacgcc gtggtgcggt gcgggtcgcc ggcgccgacc aggcggtcat agccgaaccg
     1561 ctcgagctcg gggttggtgg cgttccacgg gtctgccgcg taggccggga gggccccaag
     1621 gcccagcagg actgcagcta gcgcgcccca ccccacacct cgaacgagca tcgatgacag
     1681 gcaatgtgga cggttcatat ggagcgaacc ccttcctcac cgatccgcgg cccaggcccg
     1741 gcctaccgat ctcacgccta gtgcttactc ctcgacccac ggcccgcacg ctgccggggc
     1801 cgtggcgctg gttccgcatc gattgtccgg cgacggcgcc gacgcacaac cagggccccg
     1861 gcgccaagcg ccgccgcccc gatgccgaca cccatcagcg cgggcaccgc atattgacct
     1921 atcgatttcc ctgtcgcctc gccagcccgt ggcttgtcgg gtggcttcac ctcgtacggc
     1981 gggagccacc ccggcagatg tttcttcgcc acgagctcct cattggcctt ccacttggcc
     2041 tcgtcccact cgtagaggaa gaagtagtcg tcgaggttca ccggaccctc gcggcggggt
     2101 agctcgtact gaccgcccgg gccccaggtg accacggcca ccggaggtgc gttggcctgg
     2161 agcgccccgg cgtcgtcata cagatggatc ttcagcgcgc ggagcatgtc gtgggacgcg
     2221 ttgagttcgt ggccgatgcg taccgcggcc tggcgcgcga tctcgctgac gatgggcgtg
     2281 gacaccaggg ccacgacctc cagccgccgt ccggtcttgg aggcagttcc ttcatcaatt
     2341 gaacgacc
//
LOCUS       DAOSPL010000741         2347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45469_length_2347_cov_2.940663, whole genome shotgun sequence.
ACCESSION   DAOSPL010000741 DAOSPL010000000
VERSION     DAOSPL010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45469_length_2347_cov_2.940663"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..1120
                     /gene="pyrF"
                     /locus_tag="PLQ54_13120"
     CDS             200..1120
                     /gene="pyrF"
                     /locus_tag="PLQ54_13120"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOS94253.1"
                     /translation="MNFADRLFQRVRDTNSRVVVGLDPAWDSLPDPYRAGRGREGQAQ
                     CVESFCRRIIDAVSGLCAAVKPQAAFFEALGAPGWSALEGVCAHAHAAGIPVILDAKR
                     GDIGSTSAAYARGLLIPAGPLDLGTNVDALTVNPYLGSDGIGPFIDACRAHDKGLFVL
                     VKTSNPSSAELQDLQLSSAGRPMYEAVASAVSCWGGALQGVLGYSAIGAVVGATHPAV
                     LEDLRDQFPRVPFLVPGFGAQGAQAADVARAFGPEGLGAVVNSSRGILFAFTQEPYRS
                     EYGGDRFDQAAADACRRMRDEINLAADRKS"
     gene            1252..2031
                     /locus_tag="PLQ54_13125"
     CDS             1252..2031
                     /locus_tag="PLQ54_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94254.1"
                     /translation="MAAGTASFAQEDNAAVQTAMDKMLAAATKGDTAALKAMLHEGFA
                     GAVVTTFGNPTVCDRDAFVKAVIEQSTGAPAGLTVKLDTTETSGSLTFVKASVPVFGN
                     QGPAFWAVLAKQGEELVGVAAVLDMEPSRTTDAVKEKQSQELEEVLAGAMNFKEKGFA
                     GLVDYLSPDACILGLDMNGVLNTVAGRDKVEGMVGGIPLPESVSLSNQKVEQGYGLAS
                     AIMDLDLTMGGFSMKVKAAAYLAKTKDGWKVFAATMIPQQG"
BASE COUNT          392 a          828 c          764 g          363 t
ORIGIN      
        1 acccgacatg ccttagaccc gcggactcgg catttgttgg cgccaacgcc aagagggaca
       61 cgaagaccgc cccggccatg cctcatcggc cgcgggacgg tatcgcggcg agtcgatcca
      121 cccggtaaca tgataccgca cttggccccc gggtgcgccg cctcgcctgc cgccgaaggg
      181 gccgccgacg gagggcccga tgaacttcgc cgaccgactg ttccagcggg tacgcgatac
      241 caactccagg gtcgtggtgg gcctcgaccc ggcttgggac tccctgccgg acccgtaccg
      301 ggccggccgc ggccgcgagg gccaggcgca gtgcgtcgag tccttctgcc gccggatcat
      361 cgacgccgtc agcggactct gtgccgccgt taagccccaa gctgccttct ttgaggccct
      421 tggcgcccca ggttggtcgg cactcgaggg cgtatgcgcc cacgcccacg ccgcggggat
      481 tcccgtcatc ctcgatgcca agcggggcga catcggcagc accagcgcgg cctacgcccg
      541 cgggctcttg atccccgcgg ggccgctcga tctgggcacc aacgtcgatg cgctgaccgt
      601 caacccctac ctgggcagcg acgggatcgg gcccttcatc gacgcctgcc gcgcccacga
      661 caaggggttg ttcgtactcg tgaagacatc gaacccctcg tccgcagagc tccaggacct
      721 tcagctgtcg agtgccggcc gccccatgta cgaggcggtc gcctccgcgg tgtcctgctg
      781 gggcggggcg cttcaggggg tgctcgggta ttcggctatt ggggccgtcg tcggggccac
      841 acatcccgcc gtcctggagg acctgcgcga ccagttcccc cgagtgccat tccttgtgcc
      901 gggcttcggc gcccaaggcg ctcaggcggc cgatgtggcc cgggccttcg gccccgaggg
      961 cctgggagcc gtggtcaact cctcacgggg gatcctcttc gccttcaccc aagagcccta
     1021 ccggtccgaa tacggcggcg accggttcga ccaggccgcg gccgacgcct gtaggcggat
     1081 gcgcgacgag atcaacctcg ccgcggacag gaaatcgtga ttggccctga aaaggcggcc
     1141 cgtgtgcgtc gtagaacgaa caggcgggac cagaccgcca gactgcttcc ggaaggttgt
     1201 gatcgcgtga cactcaggtc caagtgcctc gtcgggacgc tcctggcgct cgtggccgcc
     1261 ggcacggctt ccttcgcaca ggaagacaac gccgcggtcc agaccgccat ggacaaaatg
     1321 cttgccgcag ccaccaaagg cgacacggcg gcactcaagg ccatgctcca tgaaggattc
     1381 gccggcgccg tcgtgaccac gttcggcaac cccactgtct gcgaccgaga cgccttcgtc
     1441 aaggccgtca tcgagcaatc gacaggggct ccggccggac tcaccgtcaa gctcgacacc
     1501 accgagacgt ccggctcact caccttcgtc aaggcgtccg tgcccgtctt cggtaaccag
     1561 ggccccgcct tctgggccgt gctggccaag caaggcgagg aactggtcgg cgtggccgcg
     1621 gtgctcgaca tggagcccag ccgcaccacc gatgccgtca aagagaagca gagccaggag
     1681 cttgaggagg tcctggccgg ggccatgaac ttcaaggaaa aggggtttgc cggcctcgtc
     1741 gactatcttt cgccggatgc ctgcattctg gggctcgaca tgaacggtgt gctcaatacc
     1801 gtcgcgggcc gcgataaggt cgagggcatg gttggcggga taccgcttcc cgaatcggtc
     1861 tcgctctcaa accagaaagt ggagcaaggc tacggcctcg catcggccat catggacctc
     1921 gatctcacca tgggcggctt cagcatgaag gtcaaggcgg cggcgtacct cgccaagacc
     1981 aaggacggct ggaaggtctt cgccgccacg atgatcccgc agcagggcta gcgcttccgg
     2041 ctggcaatcg gtctgcagac gggggccgga actcggcccc cgtctgcgtg tctcctatgc
     2101 atgaccgtcc gcggcggcac acggagcacc gccgcacgca cagcgctgga ccgcggatgc
     2161 gccatgggcg cgtcgtcgca ggacgtcttg agcacctgcc ccgtccgccc caccgggccg
     2221 ccaggcccgt caccacgtcg cggacgttca ggtcgatgcc gatgcacaga cgcatgcgac
     2281 ggtcacgaca cgcgacacga gtgcttcccc gggtgaccca tgggcctagg ccgacccctc
     2341 gaggtga
//
LOCUS       DAOSPL010000742         2346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45492_length_2346_cov_3.752510, whole genome shotgun sequence.
ACCESSION   DAOSPL010000742 DAOSPL010000000
VERSION     DAOSPL010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45492_length_2346_cov_3.752510"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PLQ54_13130"
     CDS             complement(<1..613)
                     /locus_tag="PLQ54_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004783515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HOS94255.1"
                     /translation="MAQCTAPVEGHRTASGAANCPACRGGRSYYPSYYPTYTPSYTPA
                     GGGGAKTVSGGSSRGSRPRWSSSSSPVTYSPAEVRALTPVREAIEAQGVLPDRYDLFL
                     CHAWDDRREKAKELHDALEDIGVSVWFSEKNVVLGTPLMREIDRGLAKSRAGLVLVTS
                     SFVKRLKAAGVADKELSALLARDLLVPVVHGITYEELRDESPLL"
     gene            774..1097
                     /locus_tag="PLQ54_13135"
     CDS             774..1097
                     /locus_tag="PLQ54_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94256.1"
                     /translation="MAWTTIRTAAALDLPAFAEPVEIHHDPAQHALLALDPATGESEV
                     LTTRLPDFPLLPDEAVVKDWSEHSGLPRALAEVGVVELLDAIAVGPFAATAHRVRVLT
                     AEDAA"
     gene            1094..1657
                     /locus_tag="PLQ54_13140"
     CDS             1094..1657
                     /locus_tag="PLQ54_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HOS94257.1"
                     /translation="MSTATPTDIGTPRVWLGCLGCYNDGALVGRWLDCTDVEDTTLAD
                     LHAGSGRPYAACEEVWVLDHEFVPVEGEFGPLEAAQWGRCYEEAGSDRWTAVCAWVRS
                     GMHVVEGTGDMPSLGDFEERYCGHWEGFREYAEQLADDTGMMTGWPEEAVRYFDWAGW
                     TRDLAFDHTVVDAPAGEGCGVYVFRNL"
     gene            complement(1663..1929)
                     /locus_tag="PLQ54_13145"
     CDS             complement(1663..1929)
                     /locus_tag="PLQ54_13145"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOS94258.1"
                     /translation="MPAPAPDLRALGVEVARLRKESGFSIDRLAEAAGVHRKTIIEVE
                     AGRVAAKISTLHGIAHALGVPLTELVAPVCARHPKQVGVPSKLV"
     gene            2040..>2346
                     /locus_tag="PLQ54_13150"
     CDS             2040..>2346
                     /locus_tag="PLQ54_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94259.1"
                     /translation="MSHRPTILTASVLLSLLALTGCSGGSAPSAGSEPTVSSAETTST
                     AETAATVEPSFYTARVIDVLSGDTLRVQMETAVYRPSDSATGEREEVEVPSETIVVKD
                     "
BASE COUNT          383 a          815 c          793 g          355 t
ORIGIN      
        1 ccaagagggg actctcgtcc cgaagctcct cgtacgtaat accgtggacg acaggcacga
       61 gcaggtcacg ggccagcaat gccgagagtt ccttatcggc gacgccggcc gccttcagcc
      121 gcttgacgaa cgaagacgtg acgagcacca ggccagcgcg cgactttgcg agcccgcggt
      181 ctatctcgcg cataagcggc gtcccgagga ccacattctt ctcgctgaac cagaccgaca
      241 ctccgatatc ttcgagcgcg tcgtgaagtt ccttcgcctt ctcccgccgg tcatcccagg
      301 catggcaaag aaagaggtca tagcgatctg gcagcacccc ctgggcttcg attgcttccc
      361 tgaccggagt cagcgcccgg acctctgcag gtgagtaggt caccggcgaa gaactggaag
      421 accaacgggg gcgcgatccg cgggaggagc cgcccgaaac cgttttggcc cctccaccac
      481 ccgcaggcgt gtagctgggt gtgtatgtcg ggtaatacga ggggtagtag gagcgtcccc
      541 cacggcatgc cgggcagttc gctgcgccgc tggcggtcct gtgtccctct acgggtgctg
      601 tgcattgagc catagcaaga gcgtagttgc agcgaccgac attcaggggg agagcaccgc
      661 tggcgcggtg ccgggcctcc tggtgaggac ccggctgtgc ccgcaagcgc ctattcaccc
      721 gagcacggct gcgagggtgc gccgggtcct ccccgacacc gaggaggacc gccatggcct
      781 ggacaacgat ccgcaccgcc gccgctctcg acctgcccgc cttcgctgag ccggtcgaga
      841 tccaccacga ccccgcgcag cacgccctgc tggccctgga ccccgccacc ggcgagtccg
      901 aggtcctgac cacccgcctg cccgacttcc cgctgctgcc cgacgaggcc gtcgtcaagg
      961 actggtccga gcacagcggg ctcccccgcg ccctggccga ggtaggcgtc gtcgagctgc
     1021 tcgacgccat cgccgtcggg ccgttcgccg ccacggcgca ccgcgtgcgc gtgctgaccg
     1081 cggaggatgc ggcatgagca cggcgactcc cacggacatc ggtactcccc gggtgtggct
     1141 cgggtgcctg ggctgctaca acgacggggc cctggtgggc cggtggctcg actgcaccga
     1201 cgtggaggac accaccctcg ccgacctcca cgcgggctct gggaggccgt acgcggcctg
     1261 tgaggaggtc tgggtgctcg accacgagtt cgtgcccgtc gagggcgagt tcgggcctct
     1321 ggaggccgct cagtggggcc ggtgctacga ggaagccggc tccgaccgct ggacagccgt
     1381 gtgcgcgtgg gtccggtcgg ggatgcacgt ggtcgagggc accggcgaca tgccgagctt
     1441 gggcgacttc gaggagcgct actgcgggca ctgggagggc ttccgggagt acgccgagca
     1501 gctcgccgac gacaccggga tgatgaccgg ctggccggag gaggccgtgc ggtacttcga
     1561 ctgggcgggc tggacccgcg acctcgcctt cgaccacacc gtcgtcgacg ccccggcggg
     1621 cgagggatgc ggcgtgtacg tgttccgaaa cctgtgagcg gctcagacga gcttggaggg
     1681 cacgcccacc tgcttggggt gccgcgcgca gaccggggcc accagctcgg tcaagggcac
     1741 gccgagggcg tgggcgatgc cgtggagcgt ggagatcttc gcggccacgc gcccagcctc
     1801 gacctcgatg atcgtcttgc ggtggacccc ggcggcctct gcgagccggt cgatcgagaa
     1861 cccggactcc ttgcgcaggc gcgccacctc cacgccgagg gcgcgcaggt ctggggcggg
     1921 agcaggcatc cacccaactt cccgcaggcg cgtgcgttgc acagtaaccc aaacgcgcct
     1981 gttttcagta cgctgatcgg gagttcaacc gaaccattgt cgaacgaagg tgaaccacca
     2041 tgtcccacag accaacgatc ctgactgcat cggtcctgct gtcgctgctt gccctcacgg
     2101 ggtgctccgg tggaagcgcc cccagtgccg gttctgaacc aactgtctcc agcgcggaga
     2161 ccacatctac cgcagaaacc gccgctaccg tcgagcccag cttctacacc gcccgcgtca
     2221 tcgacgtgct cagcggtgac acgctgcgcg tgcagatgga gaccgccgtc taccgcccca
     2281 gcgactccgc caccggagag cgcgaggagg tcgaggtgcc cagcgagacc atcgtcgtca
     2341 aggacc
//
LOCUS       DAOSPL010000743         2343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45565_length_2343_cov_3.595280, whole genome shotgun sequence.
ACCESSION   DAOSPL010000743 DAOSPL010000000
VERSION     DAOSPL010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45565_length_2343_cov_3.595280"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="PLQ54_13155"
     CDS             complement(<1..1098)
                     /locus_tag="PLQ54_13155"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOS94260.1"
                     /translation="MDELMAQRVRKLEELRRMGMDPFQITRFDRTHTASEVHRRFDAL
                     EGKAVTLAGRLVARRGHGRAAFLDLADHTGRIQLHVTEDRLGPEAYGRLDLVDLGDFI
                     GVSGEVFRTRRGEVSIAVSELRILSKSLRPLPEKFHGLRDAEAKHRRRYLHLIAEPEV
                     RDLFVARSRMITAIRHLLDERGFVEVETPMMQAIHGGAAARPFVTHHNALDIDLYLRI
                     APELYLKRLIVGGFDRVYEINRNFRNEGMDRDHSPEFTMLELYQSYADYHQIMELTTE
                     IIRTAAVAVSGAMKARFAGHEIDLGADWHVRTMDEVLREFAGVSLADLSDDSAAKAVA
                     RAKEVDPDGPVTVATVLNKVFDIYVQPNLVEP"
     gene            complement(1209..1685)
                     /gene="greA"
                     /locus_tag="PLQ54_13160"
     CDS             complement(1209..1685)
                     /gene="greA"
                     /locus_tag="PLQ54_13160"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOS94261.1"
                     /translation="MDNDEIVITKEGSERLQAELRRLTTIDRPEATARVREAKRSAED
                     FDTSEYENAKMDQAIIEGRIEEIRSILRRSVVLRDEDIPTDVVGIGSHVRVKDLDLDE
                     SWDLRIVGPIEADPSEDRISYESPVGSALMGKKVRDTIEVKVPDGTIKYKITKISK"
     gene            2123..>2343
                     /locus_tag="PLQ54_13165"
     CDS             2123..>2343
                     /locus_tag="PLQ54_13165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOS94262.1"
                     /translation="MAIGPMSRRQFLKTTGAALGGAAVPLILPTSRAFGANDRVVIGL
                     MGCGGRGDFLAEFLASRTDCRIAYVSDADS"
BASE COUNT          392 a          812 c          730 g          409 t
ORIGIN      
        1 gggctcgacg aggttcggct gcacgtagat gtcgaacacc ttattcagca ccgttgccac
       61 cgtgacgggt ccgtcggggt caacctcctt ggcccgggcg acggccttgg cggccgagtc
      121 gtcgctcagg tcggccagtg agacgcccgc gaactcccgg agcacctcat ccatggtacg
      181 gacatgccag tccgcaccca ggtcgatctc gtggccggcg aaccgggcct tcatcgcgcc
      241 cgacaccgcc accgcagccg tgcggatgat ctccgtcgtc agctccatga tctggtgata
      301 gtcggcatat gactggtaga gctcgagcat ggtgaactcg gggctgtgat cgcggtccat
      361 gccctcgttg cggaagttgc gattgatctc atacacacga tcgaaaccgc ccacaatcag
      421 gcgtttgaga tagagctcgg gcgcgatccg caggtacagg tcgatatcga gagcattgtg
      481 atgagtgacg aacggtcgag cggccgcccc gccatggatg gcctgcatca tgggcgtctc
      541 gacctcgacg aagccccgtt cgtcgagcag gtgccggatg gctgtgatca ttcgggaacg
      601 tgccacaaag aggtcgcgga cctcgggttc cgcgatgagg tgaaggtaac gccgacggtg
      661 cttcgcctcg gcgtcacgca agccgtggaa cttctcgggc agcggccgga gcgacttgct
      721 caggatccgc agctcggaaa ccgcgatcga gacctcgccc cgtcgtgtgc ggaacacctc
      781 gccacttaca ccgatgaagt cacccaggtc gacgaggtcg aggcgtccgt atgcctcggg
      841 acccagacgg tcctccgtaa catggagctg gatgcggccg gtgtggtcgg ccaggtccag
      901 gaaggccgcg cggccgtgcc cgcggcgtgc caccagcctt ccggccaggg ttaccgcctt
      961 gccttcaagc gcgtcaaagc ggcgatgaac ctcgctggcc gtgtgggtcc gatcgaaccg
     1021 agtgatctgg aacggatcca tgcccatgcg acgcagttcc tcgagcttgc gaacgcgctg
     1081 cgccatgagt tcgtccacgt ctgtcccctc ctcccgcggc taccgatggg agacacagag
     1141 aacccggcgt cctcgctgtc gggtcgaggc gccgggtccc caatcctggc cggaaggccg
     1201 acggtcccct acttgctgat cttcgtgatc ttgtacttga tggtcccgtc gggcactttg
     1261 acctcgatgg tgtcgcggac cttcttgccc atcagggcgc tcccaaccgg cgactcgtag
     1321 gagatccggt cctcggacgg atcggcctcg atgggcccca cgatgcgcag gtcccacgac
     1381 tcgtccaggt ccagatcttt cacccgcaca tggctcccaa tcccaaccac atcggtaggg
     1441 atatcctcat cgcgcaggac caccgaacgc cgcaatatgc tgcggatctc ctcgatgcgc
     1501 ccctcaatga tggcctgatc catcttcgca ttctcgtact cggaggtgtc gaagtcctcg
     1561 gccgagcgct tcgcctcgcg cacccgggcc gtggcttcgg gccggtcgat ggtcgtcagg
     1621 cgccggagct ccgcctgcaa gcgctcagac ccttccttgg tgatcacgat ctcgtcgttg
     1681 tccatggtca ccgccgcatc tcagagcaac caatggtgcg ccgctggccc cgagggactg
     1741 ggccttacgc cgttcgggcc gccgctcgga acggcagatt ctaacggccg gacccaccca
     1801 cgtcaacgcg gccatgccgc gcggcgcggg tttccgccac accccaacgg cttcctgcac
     1861 ccgtatgttc cgcgatcgcc gcccaaactc attccccggg atgcggaagt ccaaacgtgg
     1921 tggcggaaca ggaggcccga ggctcccggc ccaggcgacg acacacaccg cactttgggg
     1981 gctgcgccca ggctgcccgg agcccccgaa ggggcggcca cccgcgcacc gggccaacct
     2041 tccccaaggg actctccctc gcctggcgga acacttgctc gactcagcca cgtggtgaaa
     2101 accccactag gaggcgcgag acatggcgat cggaccgatg agccgcaggc agttcctcaa
     2161 gaccaccggc gccgctctcg gcggggctgc ggtgcctctc atcctgccca cgagccgggc
     2221 attcggcgcc aacgatcgcg ttgtgatcgg cctgatgggc tgcggcggcc gcggcgactt
     2281 cctggccgag ttcctcgcgt cccggaccga ctgccggatc gcctatgtca gcgatgccga
     2341 ctc
//
LOCUS       DAOSPL010000744         2339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45666_length_2339_cov_3.442207, whole genome shotgun sequence.
ACCESSION   DAOSPL010000744 DAOSPL010000000
VERSION     DAOSPL010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45666_length_2339_cov_3.442207"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2339
                     /locus_tag="PLQ54_13170"
     CDS             <1..>2339
                     /locus_tag="PLQ54_13170"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOS94263.1"
                     /translation="DGPAALDAALEYPAPGLRRVSGVFGSAQGFSGNHKLRVVGGPAV
                     GGTSAVSITAWVLPADLTGFREILRKEDADQRILFSFQADGTILSLGLNIGGYVECDA
                     PVEPSQVADGRWHLCAATFDGRTIRVYFDGAEIGSLDRPGTIAAGGPAPTCIGSSDGG
                     ECFQGQMDELTVYAEALSAAEIAELYRSGTDALARQGSAEPDAGPVIAKRLAAHWTMD
                     EPSGAAELRDAEGDHPAAAQPGTPLFRAPGVHGSALALHGPHAVSTDRGLLTEPVVAL
                     SCSAWVRPTDLSGFREILRQECARRLLFSFQEGGTVLSLGLNVGGYIECDAPIDPARV
                     ADGRWHHCAGTFDGQTMRVFLDGSEIGSLERTGPVSVDADAPVFIGSSSGESEHFQGD
                     LDEVRVYAEALTGDDVAALYRAGTRVLQQRDVAIQSRLQALYTQEDTFARTLAGLRRR
                     IIDERMPVDQDVADAVSRQLASAFPREVRDFTAWTGMTPLECVASRRGDALAGAVERL
                     ADLAVEYRPLTDEQWSRQTDEDRRRWAQVDAIKERVDRLLADDRSEHDWAEWVEVALD
                     LGSRVSFRPYLAEPVAPYVKPETPKTGRLSESEAREALERDWLHQAGGAPTPDRVRQE
                     IGWARDLAQRLRWSGADVRVLDAAVAELDECEDAARDLPAPNAELYFRVRAAKRRIVF
                     ANPALDFARVLFVDMPYPQGSEWAHETRHRLGYMAVPGARLMVLDGLSPHGVPTQLMP
                     KAPLHGSFWRPDVSFDGRRVLFCFKPHNEKSFHLYEIHA"
BASE COUNT          332 a          792 c          810 g          405 t
ORIGIN      
        1 acgacggccc tgcggctctg gacgcggctc tcgagtatcc ggcgcccggt ctgcgccgtg
       61 tgtctggcgt gttcggctcg gcccagggct tctcgggcaa ccacaagctg cgcgtggtcg
      121 gcgggccggc cgtcggcggc acctcggctg tctctatcac cgcatgggtc ctgcccgccg
      181 acctcaccgg cttccgcgag atcctccgca aggaggatgc tgatcagcgg atcctcttct
      241 ctttccaggc tgacggcacc atcctctctc tgggcctgaa catcggcggc tatgtcgagt
      301 gtgatgcccc agtggagccg agccaagtcg cggacgggcg atggcatctg tgcgcggcca
      361 ccttcgacgg taggacgatt cgcgtctact tcgatggcgc agagatcggg tcgctcgatc
      421 ggcctgggac catcgctgca ggcggtccgg cgcccacctg catcggttcg tccgatggcg
      481 gcgagtgctt ccaggggcag atggatgagc taacggtgta cgccgaggcc ctctcggccg
      541 ccgagatcgc ggagctgtac cgctctggca ccgatgctct cgctcgccag ggctcagcag
      601 agccggatgc tgggcccgtc atcgcgaaac gtcttgccgc ccattggacc atggatgagc
      661 ccagcggcgc ggccgagctg cgcgatgccg agggagacca tcccgccgcc gcacagccgg
      721 gcactccact gttccgcgcg ccaggagttc atggcagcgc attggccctg cacggtccgc
      781 acgccgtgtc gacggatcgg gggctcctca ccgaaccggt ggttgcgctc tcctgctccg
      841 cctgggtacg gccgacggac ctgagcggtt tccgtgagat cctccggcag gagtgcgctc
      901 ggcgtctgct cttctcgttc caggagggcg gcacggtgct ctcgctcggc ctcaatgtcg
      961 gcggctacat cgagtgcgac gcccccattg acccggcccg tgtcgctgac ggcaggtggc
     1021 accattgcgc aggcacgttc gacggccaga cgatgcgggt gttcctcgat gggtccgaga
     1081 ttggatcgct ggagcggacg ggccccgtgt ccgtcgatgc cgatgcgccg gtgttcattg
     1141 gatcctcgag cggtgagtcg gagcacttcc agggagacct cgacgaggtt cgcgtctacg
     1201 ccgaggcgct caccggcgac gacgtggccg ctctgtaccg tgcggggact cgggtcctgc
     1261 agcaacgaga cgtggcgatc cagagtcggc tccaggccct gtacacgcag gaggacacat
     1321 tcgcccggac cttggccggc ctgaggcggc ggatcatcga tgagcgtatg cctgtggacc
     1381 aggacgtcgc tgatgccgta tcgcggcaac ttgcctcggc gttcccacgg gaggttcgcg
     1441 atttcaccgc gtggaccggg atgacgcccc tggaatgcgt cgcctctcgc cggggcgatg
     1501 ccctggccgg cgccgtcgag cgcctcgccg atctggcggt cgagtatcgc cctcttaccg
     1561 atgagcagtg gtcgcgccag accgatgaag accggcgccg gtgggcccag gtcgacgcca
     1621 tcaaggagcg cgtcgacaga ctcctggctg acgacagaag cgagcatgac tgggccgagt
     1681 gggtcgaggt cgcactggat ctcggcagcc gggtgagttt ccgcccatac ctcgccgagc
     1741 ccgtggcgcc ctacgtgaag cccgagacgc ccaagactgg gcggctgagc gagagcgagg
     1801 cgcgggaggc cctcgagcgc gactggctcc accaggccgg aggcgcgccc acccctgatc
     1861 gggttcggca ggagatcggc tgggcccgtg acctggctca gcgcctgcgg tggtcagggg
     1921 ccgacgttcg ggtcctcgac gcggctgtgg ccgagctcga tgagtgcgag gacgccgcgc
     1981 gggacctgcc tgcgcccaac gcggagctct acttccgggt ccgcgcggcg aagcgccgca
     2041 tcgtgttcgc gaacccggcg ctcgatttcg cccgcgtgct gttcgtcgac atgccctacc
     2101 cgcagggatc cgagtgggca cacgagaccc ggcatcgcct gggctacatg gcggtgcccg
     2161 gagctcgcct gatggtgctc gatggcctct ctccccatgg agtgccgact cagctcatgc
     2221 cgaaggcgcc cttgcacggg tcgttctggc ggcccgatgt gtccttcgat ggccggcgcg
     2281 tgctgttctg cttcaagccg cacaacgaga agtcgttcca tctctacgag atccacgcc
//
LOCUS       DAOSPL010000745         2337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45727_length_2337_cov_3.169588, whole genome shotgun sequence.
ACCESSION   DAOSPL010000745 DAOSPL010000000
VERSION     DAOSPL010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45727_length_2337_cov_3.169588"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLQ54_13175"
     CDS             complement(<1..315)
                     /locus_tag="PLQ54_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94264.1"
                     /translation="MQLPPVPERHAVTDSGRYWSPLDDGSAVLWSSGQGEDQWLLGDG
                     SGWREVPTTDPLLTKAFCGLRIGHEPFDLEESLQHSAGANRWMCAALPDGTRIDVSGP
                     PSH"
     gene            complement(525..2168)
                     /locus_tag="PLQ54_13180"
     CDS             complement(525..2168)
                     /locus_tag="PLQ54_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94265.1"
                     /translation="MVKFELQTSYGQPNWYPCSVTFYAPLVFAANGSPDPQRQGVYTA
                     NWAAQSYGHNGDYDAKAIVHWVKQGYPPQSNDEILWQGFEVDNLIVRVDITTQVSPDP
                     YVFAYDPANPDLDSPQIAWQILGSFAPETQNLLTHTIQICSSGGSVVWEESGYSPATI
                     GTVFWDGKTSAGQPCPAGLYSVMMSASRVGLQDGHSLRQCDVTVATVPGKPLAVRYQK
                     FAVGTVKFQFNYSLTDPTGNGADSVTATLYGPNLQPVGQDVALVGVPGTYELYGTFCG
                     IDFTEGGTYTVLITYKEKTTEHSRWNKDHEADFGMPLAATFHVPYAVACSGSGVAQEE
                     GLVQQYMGGLDGPTWYATKVVPDSQNAPKDTPRLRATVNAHRPAIVYVGAHGEGAHHA
                     TLMIKAAEWILVPYDAADPVADLPMHSGEPEPVASASHPDYCWDLSQCTVAVFAACYS
                     GLPDAHGVFRDASVSRGAQAGVDFTDMLNGGTDWCNFFFGHLCVGPKLFGCSPDGPQP
                     LCVALNEARWHYYQAFGEYGGYDTYMIAGNAGAMVVPAP"
BASE COUNT          394 a          752 c          800 g          391 t
ORIGIN      
        1 gtgcgacggt ggcccggaga cgtcgatccg cgtgccgtcc ggcagggccg cgcacatcca
       61 gcggtttgcg cctgcggagt gctgcagaga ctcctcgagg tcgaacggct cgtgtccgat
      121 cctgagcccg cagaaagcct tggtgagaag gggatctgtt gtaggcacct cgcgccagcc
      181 cgagccgtcg cccagtagcc actggtcctc tccctgaccg ctggaccaca acaccgctga
      241 cccatcgtcc aggggagacc agtaccggcc cgaatcggtc accgcgtgcc gctcgggcac
      301 cggcggcagc tgcacagcgc ggcgccggtc caggccgccg agcggcggga gcaccgtgat
      361 ggagaaatgt tcccgtgtcg cgttcgccac agggcgcgat gtagggcgtg tgcagcccgc
      421 aatcagtagg gcgccaaggg caagagcgaa cggcgccagc catcgcgggg ccgtgatcgg
      481 tctctccctc gcggggcggc ggaacgtctg ggcagaccgc acggtcacgg cgctggcacc
      541 accatagcgc cggcgttgcc ggctatcatg tatgtgtcgt agccgccgta ctcgccgaat
      601 gcctggtagt agtgccaccg agcctcgttg agcgccacac agaggggctg cggcccgtca
      661 ggcgagcacc cgaagagctt ggggccgacg cagagatgcc cgaagaagaa gttgcaccag
      721 tccgtgccgc cgttcagcat gtcggtgaag tcaacaccgg cctgggcccc ccgcgagacg
      781 gaagcgtcgc ggaacacgcc gtgagcatcg ggcagacccg agtagcacgc ggcgaacacc
      841 gcgactgtgc actgggatag gtcccagcag tagtcggggt gactggcgct ggcaacgggc
      901 tccggctcgc cggagtgcat aggcaggtcg gcgacagggt cggctgcgtc gtacggaacg
      961 aggatccact cggccgcctt gatcatgagc gttgcgtgat gggccccctc gccgtgggcc
     1021 ccaacgtaga cgatggctgg cctgtgtgcg ttcaccgtag cgcggaggcg cggggtatcc
     1081 ttgggggcgt tctgggagtc cggcaccacc ttcgttgcat accacgtggg cccatcgagg
     1141 ccccccatgt actgctgcac gaggccctcc tcctgggcaa ccccggagcc ggagcatgcc
     1201 acggcgtatg gcacgtggaa cgtggccgcc agcggcatgc cgaagtcggc ctcgtggtcc
     1261 ttgttccatc gagagtgctc cgtcgtcttc tccttgtacg tgatcaggac tgtgtaggtg
     1321 cctccttctg tgaagtcgat gccgcagaag gtcccgtaca gctcgtacgt gccggggacc
     1381 ccaacgagcg cgacgtcctg ccccactggc tgcaagttgg ggccgtacag tgttgccgtc
     1441 acggagtccg caccgttgcc ggtcggatcc gtcagcgagt agttgaactg gaacttcacc
     1501 gttccgaccg cgaacttctg gtagcgaaca gcgaggggct tgccgggcac cgtggcaact
     1561 gtgacgtcgc actgcctgag cgagtggccg tcctggagcc ccacgcgact ggcactcatc
     1621 atcacgctat agaggccggc gggacagggc tgacctgccg acgtcttgcc gtcccagaaa
     1681 acggtgccga tcgtcgcggg gctgtacccg ctctcctccc agaccacgct gccgccgctg
     1741 ctgcagatct ggatcgtgtg cgtgagcagg ttctgcgtct ccggggcgaa tgacccgagg
     1801 atctgccatg ctatctgtgg gctgtcgaga tccgggttcg ccgggtcgta ggcgaagacg
     1861 taggggtcgg gcgagacctg agtggtgata tcaacccgca cgatcaggtt gtccacttcg
     1921 aagccctgcc agaggatctc gtcgttcgac tgcggggggt acccctgctt cacccagtgc
     1981 acgatggcct tagcgtcata gtcgccattg tggccataac tctgggcggc ccagttggcg
     2041 gtgtacacac cctgccgctg gggatcgggg ctcccgttgg ccgcgaacac caacggcgcg
     2101 tagaaggtca ccgagcaggg gtaccagttc ggctgtccat aggacgtttg gagctcgaac
     2161 ttcaccatgt tggacacgct cgcgccagac tgcggcgaga tctatgacga ggcatggcca
     2221 gcggcacaga tgacaagcga gccagcggcg aggccgacga ggaggcgcgc cccgcgccag
     2281 gcggaggtgg tgcctgcggt acgcatcgca gtcccctctc aacacaccta tggggtg
//
LOCUS       DAOSPL010000746         2336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45753_length_2336_cov_3.052609, whole genome shotgun sequence.
ACCESSION   DAOSPL010000746 DAOSPL010000000
VERSION     DAOSPL010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45753_length_2336_cov_3.052609"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLQ54_13185"
     CDS             <1..285
                     /locus_tag="PLQ54_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOS94266.1"
                     /translation="GSGVCFVSHRGDVQPCGYLPLVVGNVLERDLRDIWQHSPEFAQL
                     RDPEGLGGKCGLCEFRTVCMGCRARAYAATGDIMAEEPFCIYEPKRLRRD"
     gene            complement(282..1040)
                     /locus_tag="PLQ54_13190"
     CDS             complement(282..1040)
                     /locus_tag="PLQ54_13190"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOS94267.1"
                     /translation="MTSTALPVYPIEGTISFAIIEDTVTVIDAGVPTPSLGLHTPRAP
                     SPALQPYFAFFASHNITVTDVQLVVITGTGWAHICALGDLVRMTGARVVVHEAAAPTV
                     SGQERHPVADRLEEVGVPLRPVPVARTVRQGDRLSFLGGSHVIEMPGHGPGAIAIHSP
                     STRSLCVGDALRIAEDGGLLSPVTCPNPAARRLRQAFARRLDDLRRLAALEVRHLFAS
                     YSEVEGGPRMLEGDISGKIQGYLREFDRMLEQDS"
     gene            complement(1065..1820)
                     /locus_tag="PLQ54_13195"
     CDS             complement(1065..1820)
                     /locus_tag="PLQ54_13195"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOS94268.1"
                     /translation="MIPRIDDAEAAARALARAESILVITGAGMSAESGIPTFRGPDGL
                     WEGRSVEDVATPEGFARDPAGVWQWYSDRRRNCLQTEPHAGHVALARLEHGHRAPVTI
                     ATQNIDGLHRRAGSGDVIELHGSLFTTRCSKCSHQVPEDRAFYSEPPTCEYCGAPMRP
                     GVVWFGEMLPADRLLRAQALSQECDVVLVVGTSAVVQPVASLPLYARHSGATLVEVNP
                     QETPLTPVCHFAFPHAAGEVLPQLVDRALAMVE"
     gene            1955..>2336
                     /locus_tag="PLQ54_13200"
     CDS             1955..>2336
                     /locus_tag="PLQ54_13200"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOS94269.1"
                     /translation="MESRIPAPEMAHFCRQFGTMLSADVNLLPVVATLRQQTGNEYLR
                     EVLLGVQEDLEMGVPLAVAFSRYPQDFSPFFIQLVRQGEIEGALPDVLQRLADHYEAE
                     ARGELSGSQPEVTVNLDMALVVEAL"
BASE COUNT          372 a          755 c          830 g          379 t
ORIGIN      
        1 ggtagcgggg tgtgctttgt gtcgcaccgc ggcgatgttc agccgtgcgg ttacctgcct
       61 ctggtcgtgg ggaacgttct cgagcgcgat ctgcgcgata tctggcagca ctcgccggag
      121 ttcgctcagt tgcgcgaccc ggagggcctg ggcgggaagt gcgggctgtg cgagttccgc
      181 accgtgtgca tgggctgccg ggctcgggca tacgcagcca cgggtgacat catggccgag
      241 gagccgttct gcatctacga accaaagcgc ctgcggcgag actaggagtc ctgctcgagc
      301 atccgatcga actctcgcaa gtagccctgt atcttcccgg agatgtcgcc ctcgagcatc
      361 cgggggcctc cttcgacctc gctgtaggac gcgaatagat ggcggacctc gagtgcggcg
      421 agtcgccgca gatcgtcgag ccgcctggca aatgcctggc gcagccgacg tgcagcgggg
      481 ttggggcagg tcaccgggga tagcagacca ccgtcttcgg cgatgcggag tgcgtcgccc
      541 acacatagcg agcgggtcga tggcgagtgg atagcgatgg ccccgggccc gtggccgggc
      601 atctcgatga cgtgcgagcc gccgaggaaa gacagccggt cgccctggcg gacggtccgg
      661 gcgacgggga ccggcctgag gggcacgccg acctcctcga gccgatcggc cacgggatgg
      721 cgctcctgcc cgctcacggt cggggcagcg gcctcgtgga ccaccacacg agcgcccgtc
      781 atccgaacga ggtcgcccag ggcacagatg tgggcccatc cggtgccggt aatcacgacg
      841 agctgaacgt cggtcaccgt gatgttgtgt gatgcgaaga acgcgaagta gggttggagg
      901 gccggtgacg gtgcccgagg cgtgtgcaga cccaatgagg gtgtggggac cccggcatcg
      961 atcaccgtga cggtatcctc gatgatcgcg aacgagatcg taccttcgat tgggtagact
     1021 ggcagagccg tcgacgtcac cccttccggg ccccgaggga tgccctactc taccatggcc
     1081 agggcccggt cgacgagttg gggcagcacc tctcccgcgg catgagggaa cgcgaagtgg
     1141 cacaccgggg tcaggggcgt ctcctgaggg ttgacctcga ccagtgtcgc gcctgagtgc
     1201 cgggcgtaga gcggcaggct ggccaccggc tgaaccaccg ccgaggtacc gacaaccagg
     1261 acgacgtcgc actcctggct gagcgcctgg gcccggagca agcggtctgc gggcagcatc
     1321 tcgccgaacc agacgacgcc ggggcgcatg ggcgccccgc agtactcgca tgtggggggc
     1381 tccgagtaga atgcgcggtc ttcaggaacc tggtgggagc acttcgagca ccgggtggtg
     1441 aacagactgc cgtggagctc gatgacatcg ccgcttccag cgcgccgatg cagcccatcg
     1501 atgttctgcg tggcgatcgt gacgggggcc cggtgtccgt gttcgagtcg cgccagggcc
     1561 acgtggcccg cgtgtggttc tgtctgcagg cagttgcgtc ggcgatcgct gtaccattgc
     1621 cacacgccag ccgggtcgcg cgcgaaaccc tctggggtcg ccacgtcctc aaccgaccgg
     1681 ccttcccaga gcccatcggg accgcggaac gtcgggattc cgctctcggc cgacatgccc
     1741 gcgccggtga tcaccagtat ggactcggcc cgtgcgagag ctcgggcggc tgcctcagca
     1801 tcgtcgatcc gcgggatcac aacccacctc cgagtcgggc ccgccggcgc ctcgactcgc
     1861 gggaggatgc cgtgcccggg gaatcgaact tcgcctcaca gcaccgccgg gcctgccggg
     1921 cccgcggggg caacgtacgg gggttgtgca ggttatggag tctcgcattc cggcaccgga
     1981 gatggctcac ttctgccgcc agtttggcac catgctgtcg gccgacgtga acctgctccc
     2041 tgtggtggct acgctcaggc agcagacggg aaacgagtac ctgcgcgagg tgctcctcgg
     2101 cgttcaggaa gacctggaga tgggcgtccc actggcggtg gccttctcgc gctatccaca
     2161 ggacttctcg cccttcttca ttcaactcgt ccggcaaggc gagatcgagg gggccctacc
     2221 cgacgtgctg cagcgcctgg ccgatcacta cgaggccgag gcgcgcggcg agctgtccgg
     2281 ctcccagccc gaggtcacgg tgaacctgga catggccctg gtggtcgagg cgctgc
//
LOCUS       DAOSPL010000747         2335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45776_length_2335_cov_3.029825, whole genome shotgun sequence.
ACCESSION   DAOSPL010000747 DAOSPL010000000
VERSION     DAOSPL010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45776_length_2335_cov_3.029825"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PLQ54_13205"
     CDS             complement(<1..487)
                     /locus_tag="PLQ54_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94270.1"
                     /translation="MDCIHVQGFGTRPAAPAGRSGRLGLCAMALTVLAGASGAETELV
                     LGYYAPDRGTHYAWNEVAPGIQTFLDRDYRAAGLCCLYIHTTEGQPVRAVDLIWNGVP
                     FDDLRPSHELIWWRMLPDDLRPGEVAEVLVRPRQPLTAPANVEVRFSDGSAVSAAIAP
                     EP"
     gene            590..>2335
                     /locus_tag="PLQ54_13210"
     CDS             590..>2335
                     /locus_tag="PLQ54_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94271.1"
                     /translation="MADGWSRLTRGWPAWVLCAAVGQNAPAPAQVKDRSVFYGQELRQ
                     RARQNAERYPWAAEIRDATVQAAEPWLAYSDDELWDWMFGCTITRSWMVWSNGFCPAC
                     TKSVPMYTWEIDALGRPWKVRCPHCHEAFPKNDFAAFYRSGLDVHGVFDPQRADRRLL
                     FNAEHPDEGDPLHLFGVDDGEGFVQGDHRWRFIGAYLVHGQWKQAIVRGTTRLAEAYV
                     VTGDRRYAHKAGVLLDRVADLYPTFDFQTQALVYERPLATGYVSTWHDACEEIRELAM
                     AYDQVFDGLKDDPELVAFLSAKASEHQIENRKASFADIQRNIEDRLFQETLTNNSKIN
                     SNYPRTDIANAVMRTTLWWPANRDEVLASIEAMLKQATAVDGVTGEKGLDGYAAYAVQ
                     GVARLLGQYDLLDAGFLEETLRRVPRIADMFRFHIDTLCIGDYYPRSGDTGTYGMKAG
                     GYAGVPFSRNPGVGPSTYTLLANLHELTGDAAFAQTLVRANEGETEGLPYDLFAEDPE
                     GLQRRMRSALDQHGREPQLGSVNKQEWHLAILRSGDGPRARALWLDYDSGGRHGHLDG
                     MTLGLFGRGLDLLPDL"
BASE COUNT          412 a          747 c          787 g          389 t
ORIGIN      
        1 tcggctcggg ggcgatagca gccgagaccg cggagccgtc ggagaaccgg acctccacgt
       61 tcgcgggcgc cgtcaggggc tgccgcggcc ggaccagcac ctcggccacc tctcccggcc
      121 ggagatcatc cggaagcata cgccaccaga tgagctcgtg acttggacgc aggtcatcga
      181 agggaacgcc gttccagatg aggtctaccg cccggaccgg ctggccctca gttgtatgga
      241 tgtatagaca acatagccct gccgcacggt aatcgcgatc gagaaaggtc tggatccccg
      301 gggccacctc gttccacgcg tagtgtgtgc cacggtcggg tgcatagtag cccagcacca
      361 gctcagtctc ggcgcccgag gcgccggcga ggaccgtcag cgccatggca cacaggccga
      421 ggcggccact ccggcccgca ggagccgctg gtctggtgcc gaacccctgc acatggatgc
      481 aatccacggc gcacccccgg tgagcgagtg atcggtacca ggtgtggttc cgggtggtgc
      541 gcgcgaagcc cttgttagcg cccggcgcct acgggagagg cggtgctcca tggccgatgg
      601 atggtctcga ctcacgaggg gttggccggc atgggtgctg tgtgcggccg tagggcagaa
      661 cgcgcccgcc ccggctcagg tgaaggaccg cagtgtgttc tacggccagg agctgcgcca
      721 acgtgcccgc cagaacgcgg agcgctatcc gtgggcggcc gagatccgcg acgccaccgt
      781 gcaggccgcc gagccgtggc tcgcctactc cgacgacgag ctgtgggact ggatgttcgg
      841 ctgcacgatc acacggtcgt ggatggtgtg gtcgaacggc ttctgcccgg cctgcacgaa
      901 gtccgtgccc atgtacacgt gggagataga tgccctgggc cgcccctgga aggtccgttg
      961 cccccactgc cacgaggcgt ttccaaagaa cgatttcgcg gcgttctacc ggtcggggct
     1021 tgacgtccat ggggtgttcg acccgcagcg cgccgacagg cgtttgctgt tcaatgcgga
     1081 acaccccgac gagggcgatc cgctgcatct gtttggtgtg gacgatggcg aaggcttcgt
     1141 acagggcgac catcgttggc ggtttatcgg tgcctatctc gtccatggtc agtggaagca
     1201 ggcgatcgtg aggggcacca cgcgcctcgc tgaggcctat gtcgtcaccg gcgaccggcg
     1261 ctacgcgcac aaggccggcg tgttgctgga tcgagtggcc gacctctacc ctaccttcga
     1321 cttccagacc caagcgctgg tatatgagcg gccgctcgcc accgggtatg tctcgacatg
     1381 gcacgacgcg tgcgaggaga tccgcgagct cgccatggcc tatgaccagg tgttcgatgg
     1441 cctgaaggat gaccctgagc tcgtggcctt cctgtccgcc aaagccagcg agcatcagat
     1501 cgagaacagg aaggcctcct tcgccgacat tcagcggaac atcgaggacc gtctgttcca
     1561 agaaacgctc acaaataact cgaagatcaa ctcgaactac ccacggaccg acattgccaa
     1621 tgctgtgatg cggaccacgc tctggtggcc cgcgaaccgc gatgaggtgt tggccagcat
     1681 tgaggccatg ctgaagcagg ccacggccgt ggatggagtc actggggaga agggtctcga
     1741 cggctacgcc gcctacgcgg tccagggcgt cgcacgcctc ctgggccagt atgacctgct
     1801 cgatgccggc ttcctggaag agaccctgag gcgggtgccg cggatcgcgg acatgttccg
     1861 attccacatc gatacgctgt gcatcgggga ttactacccg cggtcagggg acacgggcac
     1921 ctacggcatg aaggcgggcg gctatgccgg ggtcccattc agccgcaacc cgggtgtcgg
     1981 cccttcgacg tacacgctcc tggcgaacct gcacgagctg accggcgacg cggcattcgc
     2041 tcagacgctg gtccgcgcga acgaaggcga gaccgagggg ctgccctatg atcttttcgc
     2101 cgaagacccg gagggcctgc agcggcgcat gcgatccgcg ctcgaccagc acggacggga
     2161 gccccagctc ggaagtgtca acaaacagga gtggcacctg gccatactac gttcgggcga
     2221 cgggccgcgc gcccgagcgt tatggttgga ctatgactcg ggcggccgcc atggccacct
     2281 tgatggcatg acgttggggc tcttcggccg gggactcgac ctgctgcccg atctg
//
LOCUS       DAOSPL010000748         2334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45797_length_2334_cov_3.977183, whole genome shotgun sequence.
ACCESSION   DAOSPL010000748 DAOSPL010000000
VERSION     DAOSPL010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45797_length_2334_cov_3.977183"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PLQ54_13215"
     CDS             <1..955
                     /locus_tag="PLQ54_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94272.1"
                     /translation="ADVIRRAWSGTRGADDRTEALPYRTAVAGAAIGFAVVVGFAVRA
                     GVAPWVALLFFGLLFAFQLTYCRIRCESGTPAIWVLPHTELRLFPFQMLGSDAFKVGG
                     SFRTLSSLTSFYFLVHGGFFNQSTVYQMESFRLADELGIRKRQMVGVGVVAVAIGLVL
                     AFWMFLWTYYSYGTNVLAGGSTSGTGGVRIAYCLQSWQQTSGQITLATTPERPRHVAA
                     LMGFSVTVGLLLARAVMLRSWLSPLGYIMATINGMQLWWSFLLAWIAKAAILRLGGVT
                     LYRRMVPLFLGLALGQFFIGGVVWGTLAIFFRDISYVIWFT"
     gene            complement(1146..>2334)
                     /locus_tag="PLQ54_13220"
     CDS             complement(1146..>2334)
                     /locus_tag="PLQ54_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOS94273.1"
                     /translation="EGACAVAKEIGYPVVVRPSFVLGGRAMEIVYDDASLREYMQRAV
                     EASPQHPVLIDRFLEDAIEIDVDAVSDGERVVIGGIMEHIEEAGIHSGDSACVLPPYS
                     LNEDELETIRDATYRLARELQVVGLLNIQFAIKSDVVYVLEVNPRASRTVPYVSKSIG
                     RPLAKIAARVMAGRSLEEQGFTEEVEIPHTTVKCPVFPFSKFPTVDPVLGPEMRSTGE
                     VMGIDEDFGRAYAKAYIAAGHNLPLRGTAFVSVRNKDKRAIIFLAKRLADLGFEIIAT
                     KGTGKVLTRNGVPARIIHRIHEAKPNILDLMAQGRIDLIINTPSGQRPKEDQARIRRD
                     AVRHGVTFVTTISGAMACVSGIEALRSGATHVRTIQQYQQRLLDHAPQGAEESRQAQY
                     AAI"
BASE COUNT          330 a          730 c          809 g          465 t
ORIGIN      
        1 ggccgatgtg atccggcggg cgtggtcggg cacgcgtggg gccgatgacc gcacggaggc
       61 cctgccgtac cgtaccgccg tggctggcgc tgccatcggg ttcgcggtgg tagtagggtt
      121 tgccgttcgg gctggcgtgg cgccgtgggt ggccttgctg ttttttggac tcctcttcgc
      181 cttccagctc acctactgcc ggatccggtg cgagtcgggc acaccggcga tctgggtgct
      241 cccacacacc gagttgcggc tgttcccgtt ccagatgctg gggtcggacg cattcaaggt
      301 gggtgggagt ttccgcaccc tgagctcgct gacgagcttt tacttcctgg tgcatggcgg
      361 gttcttcaac cagagcaccg tctatcagat ggagagcttc cgtctggcgg atgagctggg
      421 gatccgcaag cggcagatgg tcggcgtcgg cgtggttgcc gtggccatcg ggctcgtgct
      481 ggccttctgg atgttcctgt ggacctacta cagctacggc accaatgtcc tggccggggg
      541 cagcacttcg ggtactggag gcgtgagaat cgcctattgc ctccagtctt ggcaacagac
      601 ctcggggcag atcacgttgg cgaccacgcc cgagcgtccg cggcacgtgg cggcattgat
      661 gggcttctcc gtcacggtgg gcctgctctt ggcccgtgcc gtgatgctgc ggagctggct
      721 gagtccgctc ggctacatca tggcgaccat caacggcatg cagctgtggt ggtcgttctt
      781 gctggcgtgg atcgccaagg cggccatcct ccggctgggt ggagtgaccc tgtaccggcg
      841 gatggtgccc ctgttccttg gcctggcgct ggggcagttc ttcatcgggg gcgtggtatg
      901 ggggacgctg gcgatcttct tccgcgacat ctcgtatgtg atctggttca cgtgagtgcc
      961 cggcgtcccg acaacggcag cgccagtggc caacggcgga acccgggccg aaacgccccg
     1021 cgccgaacgg attcacggct actttgaacc ggccctggcc ggcccgcgtt gctcacctgg
     1081 cagccagggc cgggggcgat ccgtcgggta tcccgacagg tcgccctctc tttttccgct
     1141 gtgggctaga tcgccgcgta ctgggcctgg cgcgattcct cggcgccctg cggcgcgtga
     1201 tcgagcagtc gctgctggta ctgctgtatg gtccgcacgt gcgtggcccc cgaccgcagt
     1261 gcctcgatcc ccgacacgca ggccatggcg cccgagatag tggtcacaaa ggtcacgccg
     1321 tgccggacgg cgtcgcggcg gatccgggcc tgatcctcct tgggccgttg gcccgatggc
     1381 gtgttgatga tgaggtcgat tcgcccctga gccatcaggt cgaggatgtt gggcttcgcc
     1441 tcgtggatcc ggtggatgat gcgcgccgga acgccgttgc gggtgagcac cttgccggtg
     1501 cccttggtgg cgatgatctc gaatccgagg tcggccaggc gcttggcaag gaagatgatg
     1561 gcgcgcttgt ccttgttccg cacactgacg aacgccgttc cccggagtgg cagattgtgt
     1621 ccggcggcga tgtacgcctt ggcgtaggct cggccgaagt cctcgtcgat gcccatcacc
     1681 tcgccggtgg accgcatctc gggccccagt acgggatcga cagttgggaa cttgctgaag
     1741 gggaacaccg ggcacttcac ggtggtgtgc gggatctcga cctcttcggt gaagccctgc
     1801 tcctcgagcg atcgccctgc catgacccgt gcggcgatct tggccagcgg gcgcccgatg
     1861 gacttgctca catagggcac cgtgcgcgag gcccgcgggt tgacctcgag cacgtagacc
     1921 acatcgctct tgatggcgaa ctggatgttg aggagcccaa ccacttgcag ctcgcgcgcg
     1981 agccggtagg tggcgtcgcg gatggtctcc agctcgtctt cgttgagcga gtagggtggg
     2041 agcacgcagg cgctgtcgcc cgagtggata ccggcctcct cgatgtgttc catgatgcca
     2101 ccgatgacga cccgctcgcc atcggagact gcgtcgacat cgatctcgat ggcatcctcg
     2161 aggaaacggt cgatgagcac cggatgctgg ggcgaagcct ccacggcccg ctgcatgtac
     2221 tcgcggagcg aggcatcatc gtacacgatc tccatggccc ggccgcccag aacgaatgag
     2281 gggcggacca ccaccgggta gccgatctcc ttggcgacgg cgcacgcgcc ctcg
//
LOCUS       DAOSPL010000749         2334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45798_length_2334_cov_3.855638, whole genome shotgun sequence.
ACCESSION   DAOSPL010000749 DAOSPL010000000
VERSION     DAOSPL010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45798_length_2334_cov_3.855638"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PLQ54_13225"
     CDS             <1..509
                     /locus_tag="PLQ54_13225"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOS94274.1"
                     /translation="HLVLSTLHTNDAPGAVTRLVDMGIEPFLVASTLVGAVAQRLVRV
                     LCDNCKEPWVFDAGEYPRLGDAAALLEGKTVYRPRGCDQCRFSGYRGRTAVFEILTMS
                     EPVERLILRRASTGEIREQSISQGMRTLRQDGISRVIAGVTSVEEIIRVTQEEMENQK
                     PIVVEGVA"
     gene            511..1722
                     /locus_tag="PLQ54_13230"
     CDS             511..1722
                     /locus_tag="PLQ54_13230"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOS94275.1"
                     /translation="MPSFQYVALDATGRRVVGAVEGDDQRTAVRRLKGMGYYPTEVRE
                     AEGRSEAEGIRMGLFSRVSHGDLTTFTRQLADLVKAGLPIIRCFDALIEHTESPTLTA
                     ILQKVRDDVSGGSSLADALEKHPRVFPKVYSSMVRAGEASGQLAEILRRLALFLETER
                     ERRVAVRSMLAYPVFVITVGTIGVLSLVTVVIPRFVEIYEELGQALPGPTQVLVALSA
                     FMRGHWWELLLAVAIGQWLIRAWLRTPFMHYQIDRVKLRNRVTGKLTQKFIVARFART
                     MATLLQGGVDVLTAFAVVRDGVGNEVVARALDEVRGRIREGEMISAQLREAAVFPALV
                     PHMVALGEETGNVHGVLNSLADMYDMEVDAATKAAIAIMGPAMIAGLGVVVFFIISAM
                     LLPIFQMKVGM"
     gene            1768..2226
                     /gene="gspG"
                     /locus_tag="PLQ54_13235"
     CDS             1768..2226
                     /gene="gspG"
                     /locus_tag="PLQ54_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008043503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOS94276.1"
                     /translation="MARTDRRAQASRHARGFTITELIVVMIIIVLLAGLAVKYVPDRI
                     DEAKKSRTIADLAEAQDSLEQYRLHVGDYPTQDQGLQALVEAPSGGSESTGSWRGPYL
                     KKVPKDGWGRDLIYVRGGDGEGFEIISYGKDGKEGGEGFNADISSLKDLQ"
     gene            2156..>2334
                     /locus_tag="PLQ54_13240"
     CDS             2156..>2334
                     /locus_tag="PLQ54_13240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS94277.1"
                     /translation="MAKTAKRAARGSTRTSAASRTSSRRPRERAWGGFTIAELLVVMV
                     LIAVLAGMAMPSFMSG"
BASE COUNT          392 a          690 c          852 g          400 t
ORIGIN      
        1 gccacctggt tctgagtacc ctccacacca atgacgctcc cggcgcggtg actcgactcg
       61 ttgacatggg catcgagccg ttcctggtgg cctccacatt ggtgggcgcg gtggcgcagc
      121 gcctggtgcg ggtgttgtgc gacaactgca aggagccgtg ggtgttcgac gcgggcgagt
      181 acccgcgtct gggcgatgcg gcggcgctgc tcgaagggaa gaccgtgtac cggccgcggg
      241 gatgcgatca gtgccggttc tcgggctatc ggggccgcac ggccgtattc gagattctga
      301 ccatgagcga gcccgtggaa cggctgatcc tgcgccgcgc ctcgaccggg gagatccggg
      361 agcagagcat cagccagggc atgcggacac tgcggcagga tggcatctcg cgcgtgatcg
      421 ccggagtcac gagcgtcgag gagatcatcc gggtgaccca ggaagagatg gagaaccaga
      481 agcccatcgt tgtcgaaggg gtggcctaga gtgcccagct tccagtatgt ggcgctggac
      541 gccacgggcc gccgtgtggt gggcgcggtt gagggagacg accagcgcac ggccgtgcga
      601 cggctgaagg ggatgggcta ctaccccacc gaggtccgag aggccgaggg ccggtctgag
      661 gccgagggca tccggatggg cctcttcagc cgagtcagcc acggggatct caccacgttc
      721 acgcgtcagc tggccgacct cgtgaaagcc ggtcttccga ttatccgatg cttcgacgcg
      781 ctcattgagc acacggagag cccaacgctg accgcgatcc tgcagaaggt gcgagacgat
      841 gtgtcgggtg gctcttcact cgcggacgcg ctcgagaagc atccgcgggt gttccccaaa
      901 gtctactcga gcatggtccg agccggtgag gccagcggcc aactggccga gatcctgcgt
      961 cgcctcgccc tcttcctcga gaccgagcga gagcgacggg tggccgtccg gtcgatgctg
     1021 gcctatccgg tattcgtgat cacggtgggc accatcggag tgctgtcgct ggtgacggtc
     1081 gtgatccccc gcttcgtcga gatctatgag gagctgggac aggcgctgcc cggtccgact
     1141 caggttctgg tggccctgtc ggccttcatg cgcggccact ggtgggagct gttgctggcg
     1201 gtggccatcg gccagtggct gatccgggcg tggctgcgga cgcccttcat gcactaccag
     1261 attgaccgcg tgaaactccg taaccgcgtg accggcaagc tcacgcagaa gttcatcgtg
     1321 gcgcgcttcg cgcggaccat ggccacgctc ctgcagggcg gcgtggacgt gctcacggcc
     1381 ttcgccgtgg tgcgtgatgg ggtgggtaac gaggtggtgg cgagggccct ggacgaggtg
     1441 cggggccgga tccgcgaggg cgagatgatc tcggctcagc tccgcgaggc ggctgtcttc
     1501 ccggcactgg tgccgcacat ggtggcgttg ggcgaggaga cggggaacgt gcacggagtg
     1561 ctcaactcgc tggccgacat gtacgacatg gaggtcgatg ccgccaccaa ggcggccatc
     1621 gccatcatgg gcccggccat gatcgccgga ctcggggttg tggtgttctt catcatctcg
     1681 gccatgttgt tgccgatctt ccagatgaag gtcggaatgt gagacgtgtt gggggacggg
     1741 gtaatggctc aggaagtgac cagacgcatg gcgcggacag accgccgggc ccaggccagc
     1801 cggcacgcgc ggggtttcac gatcacggag ctcatcgtgg tcatgatcat catcgtgctg
     1861 ctggcgggac tggccgtgaa gtacgtcccg gaccggatcg acgaagccaa gaagtctcga
     1921 accatcgccg atctggcgga ggcgcaggat tcgctggagc agtaccggct ccacgtggga
     1981 gactacccga cccaggacca ggggctccag gcgcttgttg aggctccgag cgggggcagc
     2041 gagtccacgg gaagctggcg tgggccgtac ctcaagaagg tgcccaagga cggttggggc
     2101 cgcgacctga tctacgtccg cggcggcgat ggcgaaggct tcgagatcat ctcctatggc
     2161 aaagacggca aagagggcgg cgaggggttc aacgcggaca tcagcagcct caaggacctc
     2221 cagtagacgg ccccgcgaga gggcttgggg cggcttcacc atcgccgagc tcctggtggt
     2281 gatggtgctg atcgccgtgc tggcgggtat ggccatgccc tcattcatga gcgg
//
LOCUS       DAOSPL010000750         2334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45806_length_2334_cov_2.901711, whole genome shotgun sequence.
ACCESSION   DAOSPL010000750 DAOSPL010000000
VERSION     DAOSPL010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45806_length_2334_cov_2.901711"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            348..845
                     /locus_tag="PLQ54_13245"
     CDS             348..845
                     /locus_tag="PLQ54_13245"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOS94278.1"
                     /translation="MGIETTLKWLAASREFYIQMIQATPDDRLDWKPVAAQGDATSIL
                     EITRHLIANEHPMRSMVVDGKWPEDTPMDPNWAASSSFAGAGPAAEVSDKADLVRMIR
                     EAGAETDQAVRDVPDSAWNDTVSLPFMTDTRAGLVGLAASHWSYHTGQVAYLQRLYGD
                     LKFMG"
     gene            975..1661
                     /locus_tag="PLQ54_13250"
     CDS             975..1661
                     /locus_tag="PLQ54_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94279.1"
                     /translation="MTVKAMRLLLVAGMAVGVWATSTPARADGFEFSITAGDRHDDEF
                     FGLHIGWYDDDRDDPHHRYWGDFEPCKRCRDRDMRDWCDKCRNRYNNRFYDDLCDRCR
                     DRDRDHWCDKCRHRYDLRDRDDWDRDRYRDRYDGGRVSFPPGVYGGPHGLYAEPYPYE
                     ALRVVPAPDRRGYTTTIFGDRRSPFVGRPNWRDEQWRTNDWAPPAPRTTIVLPSDALD
                     GGRRRDVRRP"
     gene            1938..>2334
                     /locus_tag="PLQ54_13255"
     CDS             1938..>2334
                     /locus_tag="PLQ54_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94280.1"
                     /translation="MTERALGLLMVAGMAVGVLATSAPARADGFGLSIMAGDRHDDGL
                     FGLQIGWYDADRDRHPDHRLYWRGFDPCDRCIHRDMRDWCDWCRNRYNNRAYPDLCDR
                     CRDRDRRDWCDRCRDRYDYGYYGYSFLCDR"
BASE COUNT          364 a          761 c          833 g          376 t
ORIGIN      
        1 gcccggactc gcgtcgccgg acgcgccccc cccattgccg ttcgacgacc cgtcggtggc
       61 gccgccgggc acgatgtcca gcccgcttcc gccgcaggcg gtgatgaaga cggccagtcc
      121 gagcaccgct ggtgccagta accatagtgg cttggtccgc accatggccc ccgctccctc
      181 cctcggccgc gcgcggccac ctgatggacg tacgcccgga cggtctggcc tcgtctttcg
      241 tggtccgctg gccggcctcc tgccgctgga gggggccatt ccatggccgg cgaagggcat
      301 tacgaggcgc cggataggcg actcgtccgc cacagggagg ggcactgatg ggcatcgaga
      361 cgaccctgaa gtggctcgcg gccagtcgcg agttctacat ccagatgatc caggccacgc
      421 ctgatgaccg gttggactgg aagcctgtgg ctgcgcaggg ggacgccacg agcatcctcg
      481 agatcacccg gcacctgatc gcgaacgagc atcccatgcg cagcatggtg gtcgatggga
      541 agtggcctga ggacacgccc atggacccga actgggcggc ctcgtcgagc ttcgcgggcg
      601 ccgggccggc ggcggaggtg agcgacaagg ccgatttggt ccggatgatc cgggaggcag
      661 gagccgagac cgaccaggcg gtgagggacg tgcccgactc ggcgtggaac gacacggttt
      721 cattgccgtt catgaccgat acccgtgcgg gtttggtggg gctcgcggcc tcccactggt
      781 cgtaccacac gggtcaggtg gcctacctcc agcgtctcta cggcgacctc aagttcatgg
      841 gataggtacc cgtgccgtgg gttggggagc actcccacct gccatggaac ccctgctggg
      901 cggcccacgt cagaggggtg tgttgaacag cccgaaggca gcgggaaggg ctggcagaga
      961 agggggtggc gagaatgact gtgaaggcaa tgaggcttct gcttgttgca gggatggctg
     1021 tgggggtgtg ggccacctcg acaccggccc gagccgacgg gtttgagttc tcgatcacgg
     1081 cgggcgaccg gcacgacgac gagttctttg ggctccacat cggatggtac gacgacgacc
     1141 gcgacgatcc ccaccatcgc tactggggtg acttcgagcc ctgcaagcgc tgccgcgatc
     1201 gcgacatgcg cgactggtgc gacaagtgcc gcaatcgcta caacaatcgc ttctacgatg
     1261 acctgtgcga ccggtgccgc gatcgggatc gcgaccactg gtgcgacaag tgccggcaca
     1321 ggtatgacct ccgcgatcgc gatgactggg accgcgaccg ctaccgggat cgatacgacg
     1381 gcggacgagt cagctttccg ccgggtgtct acggcgggcc gcatggcctc tacgcggagc
     1441 cgtaccccta cgaggcactc cgagtcgttc cggcaccgga tcggcgaggc tacacgacca
     1501 cgatcttcgg cgatcgccgc tccccctttg tcgggcggcc caactggcgc gatgagcaat
     1561 ggcggaccaa cgactgggca ccgccggccc cgcggacgac tatcgtgctt cccagtgacg
     1621 cactcgacgg cggccgacgg cgggacgtcc gccggcccta gctcctgacg cgactcgggg
     1681 gcggcgccac ttggtgccgc ccccgttgtg cgtacggggt ggggcgtccg gcgcgcctgc
     1741 ctaccgtcgc atggctgggt gccgcgaccc cggccggggg cagactgatg cgggcttcct
     1801 ggcccgctag cgggcgttcg cgagccgcgg cgatcggcgc gcccacacgg aacccgcccc
     1861 gcgctgaccg cgtcagaggg gtgtggtgca cagcctgagg gtaacgagcc gggctggcag
     1921 agaagggggt ggctgggatg actgagaggg cactggggct tctgatggtg gcaggaatgg
     1981 ctgtgggggt gttggccacc tctgcgccgg ctcgcgcgga cggcttcggc ctctcgatca
     2041 tggccgggga tcggcacgat gacgggctct tcgggcttca gatcggctgg tacgatgccg
     2101 atcgcgatcg gcaccccgac caccgcctct actggcgcgg gtttgatccc tgcgatcggt
     2161 gcatccatcg cgacatgcgt gactggtgcg actggtgccg caaccgctac aacaaccgcg
     2221 cctatccgga cttgtgcgat cgctgccggg atcgggaccg gcgtgactgg tgcgaccgct
     2281 gccgggaccg gtacgactac gggtactacg gctacagctt tctgtgcgac cggt
//
LOCUS       DAOSPL010000751         2334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45809_length_2334_cov_2.851689, whole genome shotgun sequence.
ACCESSION   DAOSPL010000751 DAOSPL010000000
VERSION     DAOSPL010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45809_length_2334_cov_2.851689"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="PLQ54_13260"
     CDS             <1..1130
                     /locus_tag="PLQ54_13260"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS94281.1"
                     /translation="EHPTTCKPYADYRDMLAADDIDAVIIATLPHWHALQAIHACEAG
                     KDLYIQKPMTLHLGESLAVRNAVKKHGRISQVGTQIHASENYRRVVELIRSGNLGKVG
                     VVRTFHVMNQMPGGIGHSTDNTPPKGFDWDMWCGPAELGAYNSILVQNAYNHSSWMAY
                     SGGWTPGMAPHIIDLPIWALELGYPSEITSAGGRYLLEDDGDAYDTHDVLWRYTPEGL
                     TPGAAVPKGGLTMQWWTSLTNSYGFDLHGDPEPRRRLGIYFHGSNGTLYSDYGMHKVM
                     PEGTRMDGLVTPEPSIPPSPGHEREWLDCIRTRTQPSCSVFYHVKLDVPVVLSLLSLK
                     LGRTIRFDPKTEQIVEDKKAAQAAIPEYRDPWRFPKEYLRA"
     gene            1309..>2334
                     /locus_tag="PLQ54_13265"
     CDS             1309..>2334
                     /locus_tag="PLQ54_13265"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS94282.1"
                     /translation="MSQKTTRRTFLKQMGVAAGVAPYILSSKVWGQNVNDKLRIAFVA
                     TGGIAGAHINEMVRLGVDCPCYCDVDSDRLGAAKEHWPGAKAYADYRIMLEKHADEID
                     GVMIGTPDHHHYPATMVAMQLGLNCYTQKPLTHTPWESRRLVKMAQKNPKIVTQMGNQ
                     GHASDAWRQLYNFIRAGGIGTVLEVHTWTDRATGWWPQAMNRPEGRAPVPARNRRRTQ
                     PGDHLNWDLWLGPAPRRPFKAGAYHPFVWRGWKDFGTGALGDMGCHMMDGTVWTMDPD
                     FPTLIEPIMVKDMTAEAFPAQAIVRWTFPKKHGMPSFQQYWYESGLKPPKPEQVEGEW
                     NLPSNGSV"
BASE COUNT          449 a          768 c          764 g          353 t
ORIGIN      
        1 cggagcaccc aacaacctgc aagccctatg ccgactaccg cgacatgctg gcggcggacg
       61 acattgacgc cgtcatcatc gccaccctgc cccactggca tgcgctgcag gccattcacg
      121 cgtgcgaggc gggcaaggac ctgtatatcc agaaaccgat gacactccat ctgggcgaga
      181 gcctggccgt ccgcaacgcg gtgaagaagc atggccgcat cagccaggtg gggacgcaga
      241 tccacgccag cgagaactac cgccgggtgg tcgagctgat ccgctccggc aatctcggca
      301 aggtgggggt cgttcggacc ttccacgtga tgaaccagat gccgggcggt atcgggcaca
      361 gcaccgacaa cacgccgccc aaggggttcg actgggacat gtggtgcggc ccggccgagc
      421 tgggcgccta caactcgatc ctggtgcaga acgcctacaa ccactcgtcg tggatggcct
      481 atagcggcgg atggacgccg ggcatggcac cgcacatcat cgatctgccc atctgggcgt
      541 tggagctcgg gtatccgtcg gagatcacgt cggcgggcgg gcggtacctg ctcgaggacg
      601 acggcgacgc ctacgacacg cacgatgtgc tgtggcgcta cacgcccgaa gggctcacgc
      661 ccggcgcggc cgtacccaag ggcggcctca cgatgcagtg gtggacctca ctgaccaaca
      721 gctacggatt cgacctgcac ggcgacccgg agccgcgccg gcgcctgggt atctacttcc
      781 acggctcgaa cgggacgctg tactccgact acggcatgca caaggtgatg ccggagggca
      841 cgaggatgga cggcctggtc acgcccgagc cgtcgatccc gccctcgccc ggtcacgagc
      901 gcgagtggct ggactgcatc cggactcgca cgcagccgag ctgcagcgtg ttctaccatg
      961 tgaaactcga tgtgcccgtg gtgctgtcac tgctatcact gaagctcggc cgtaccattc
     1021 ggttcgatcc aaagaccgaa cagatcgtcg aggacaagaa ggcggcccag gcggccattc
     1081 ccgagtaccg cgacccgtgg agattcccga aggagtatct gagggcgtag agacagcgtg
     1141 cggaactggc gccgacaagg acccgccggt ctcgcgtcga acaggcatat gtcacggccg
     1201 gcgtattggg ccgttgcggc cgctctgccc gaacccgcgc gcgaggcgcc gcgttggtaa
     1261 ggccgtgggt cgggcacttc cgccacaaga tgctggaggg gacacccagt gagtcagaag
     1321 accactcgcc gcacattcct gaagcagatg ggcgttgccg cgggcgtggc gccctacatt
     1381 ctctcatcca aggtttgggg ccagaacgtt aacgacaagc tccgcattgc cttcgtcgcc
     1441 acgggcggca tcgccggcgc gcacatcaac gagatggtgc gcctcggcgt cgactgtccc
     1501 tgctactgcg acgtcgattc cgaccggctc ggcgcggcca aggagcactg gcctggtgcc
     1561 aaggcctatg ccgattaccg gatcatgctt gagaagcatg ccgacgagat cgacggcgtc
     1621 atgatcggca caccggacca ccaccactat ccggcgacca tggtggccat gcagcttggg
     1681 ctgaactgct acacgcagaa gccgctgacg cacacgccat gggaatcgcg ccggctggtg
     1741 aagatggccc agaagaaccc gaagatcgtc acgcagatgg gtaaccaagg gcacgcttct
     1801 gacgcctggc gccagctgta caacttcatc cgcgctggcg gcatcggcac cgtgctcgag
     1861 gtgcacacgt ggaccgaccg cgcgacgggc tggtggcccc aggccatgaa tcgtcccgag
     1921 gggcgggccc cggtgcccgc ccgaaatcgt cgacgcacgc aaccgggcga ccacctgaac
     1981 tgggatctct ggctgggccc ggccccgcgc cggccgttca aggcgggcgc ctaccatccc
     2041 ttcgtctggc gtgggtggaa ggacttcggc accggagcgc tcggcgacat gggctgccac
     2101 atgatggacg gcaccgtgtg gaccatggat ccggacttcc cgacgctgat cgagcccatc
     2161 atggtcaagg acatgacggc cgaggcgttt ccggcccagg ccattgtgcg ctggacgttc
     2221 ccgaagaagc atggcatgcc gagcttccag cagtactggt atgagagcgg cctgaagcca
     2281 cccaagcccg aacaggtaga gggcgagtgg aacctcccga gcaacggcag cgtc
//
LOCUS       DAOSPL010000752         2331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45880_length_2331_cov_4.095782, whole genome shotgun sequence.
ACCESSION   DAOSPL010000752 DAOSPL010000000
VERSION     DAOSPL010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45880_length_2331_cov_4.095782"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1778)
                     /locus_tag="PLQ54_13270"
     CDS             complement(<1..1778)
                     /locus_tag="PLQ54_13270"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HOS94283.1"
                     /translation="MGKAAPYPDRVLEGDSGQRTYQEPHTLQIAFPLGGIGAGCICLD
                     GGGGMRDFSIRNRPATTAVADRHAPGDAAFAVLHIKGRRPVTKLIEGPMPPERIYDQG
                     LKAQGHGSRGCDGLPRFESYEFCGEFPFARVSLAHPDVPLSVTVTGFSPFIPLDDANS
                     GIPCAILEYALSNPTRRAVEYEFSYHLSHLAQGESGPKGTRNAVIPGAGAFLYNEDPP
                     GAERFGSASLTVVGHKPAIKAMWFRGGWFDSISALWRELSTGTFRPNRGSNGVDVEGR
                     NGASILLAGTLKPGQSVTYPIVITWYFPNCYETSGPQPAHSNSCGEGQVCGPKPRWRP
                     YYAGVWKDARDVARYVHKHYASLRARTQAFHDALFSSTLPSYVLDAVSANLAILRSPT
                     VLRQENGSVWGWEGCFADQGCCPGSCTHVWNYAQAMPHLFPRLERTLREQELRHSMDE
                     RGHVTFRASLPDGPADHSFHAAADGQLGGVMKLYREWQISGDHTWLEGLYPAAKRGLD
                     YCIATWDPDRTGLLVEPHHNTYDIEFWGPDGMCSSIYIGALSAMAAMAKAVGDDEAET
                     EYRALAERGAAAMDARLFNGEYYAQQV"
     gene            1883..>2331
                     /locus_tag="PLQ54_13275"
     CDS             1883..>2331
                     /locus_tag="PLQ54_13275"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HOS94284.1"
                     /translation="MWQNQDPMVNHEILYLSQADVAQVGPSMSEIIDALETAFREKGH
                     GRTEMPPKPGIHPGGGDNFIHAMPAYIPALRSAGLKWVSGYPGNAERGLPYIAGLLIL
                     NDVDTGLPLAVMDCVWITAMRTGAATALSARYLARSDSSTVGVLGCGV"
BASE COUNT          393 a          810 c          729 g          399 t
ORIGIN      
        1 acctgctggg cgtagtactc accgttaaac aagcgcgcat ccatcgccgc cgccccgcgc
       61 tcggccagcg cccggtactc ggtctcggcc tcgtcgtcgc caacggcctt ggccatggcc
      121 gccatggccg acagggcgcc aatgtagata ctgctgcaca tcccgtccgg gccccagaac
      181 tcgatgtcat aggtgttgtg gtgcggctca accaacagcc cggtccgatc cgggtcccat
      241 gtggcgatgc agtagtccag tccacgcttg gcggccgggt acaggccctc gagccatgtg
      301 tgatcgccgc tgatctgcca ctcacggtag agcttcatca cgccgccaag ctgcccgtcc
      361 gccgccgcgt ggaagctgtg gtcggccggc ccatcgggca gtgacgcgcg gaaggtcacg
      421 tggccgcgct catccatcga gtgccgaagc tcctgctctc ggagcgttcg ttcgagcctc
      481 gggaacaggt gcggcatggc ctgagcatag ttccacacgt gggtacacga acccgggcag
      541 cagccctggt cggcgaaaca gccctcccag ccccagaccg agccgttctc ctgccggagc
      601 actgtgggcg accggaggat cgccaggttg gccgacacgg cgtcgagcac ataggagggg
      661 agggtagagg agaacagcgc atcgtggaac gcctgggtgc gggcccgaag tgacgcgtag
      721 tgcttgtgca cgtaacgggc cacatcgcga gcgtccttcc acaccccggc atagtagggc
      781 cgccaacggg gcttcggccc gcagacctgc ccctcaccgc agctgttcga gtgcgccggc
      841 tgcgggccgc tcgtctcata gcagttcggg aagtaccatg taatcacgat cggatacgtt
      901 accgactgcc ccggctttag cgtgcccgcc aacaggatcg acgcgccgtt ccgtccctcc
      961 acatccacgc cgttcgaacc cctgttcggc cggaaggtcc ccgtcgagag ctcgcgccag
     1021 agtgctgaga tgctgtcgaa ccaaccgccg cggaaccaca tggccttgat ggccggcttg
     1081 tgcccgacca ccgtcagcga tgcactgcca aaccgctcgg ctccaggggg gtcctcgttg
     1141 tagagaaacg ctccggcgcc ggggatgacc gcattgcgcg tgcctttagg gcccgactcc
     1201 ccctgcgcca gatgcgacag gtgataggag aactcgtact ccacagccct tcttgtcgga
     1261 ttactcagtg cgtactcgag aatggcgcac gggatgcctg agttggcgtc atcgagggga
     1321 atgaacgggc tgaatccagt gacggtgacc gacagcggca catccggatg ggccagcgac
     1381 accctggcaa acgggaactc cccgcagaac tcatagctct cgaagcgcgg taggccatcg
     1441 caccctcggc tgccgtgccc ctgcgccttc agcccctggt cgtagatacg ctccggcggc
     1501 atcggcccct cgatgagctt cgtcaccggc cttctgccct tgatgtgcag cacggcgaag
     1561 gcggcatcgc cgggcgcgtg ccgatcagcc acggccgtgg tagccggtct gttgcggatc
     1621 gagaagtccc gcatcccacc cccgccatcg agacagatgc atcccgcgcc gatcccgccc
     1681 agcgggaacg cgatctgaag cgtgtggggt tcctggtacg tccgctgacc cgagtcgccc
     1741 tcaagcacgc ggtcggggta cggtgccgcc ttgcccatct ctgtggcctc ctgtgctggt
     1801 gaactccgct tcgaaccacg gccgctaatg cgacggaagc cggcgagtga ccttctgact
     1861 acctccgcag gtcgcacggc gcttgtggca gaaccaggac cccatggtca accacgagat
     1921 cctctatctg tcgcaagccg acgtggccca ggtgggaccg tcgatgtccg agatcatcga
     1981 cgccctcgag acggcttttc gcgagaaggg ccacggccgc accgagatgc cccccaagcc
     2041 gggcattcat cccggtgggg gcgacaactt catccatgcc atgcccgcct acatccctgc
     2101 cctcaggtcc gctggcctga aatgggtcag tggctacccg gggaacgccg aacgtgggct
     2161 gccctacatc gctgggctgc tgatcctcaa cgatgtcgac acggggctgc ccttggccgt
     2221 gatggactgc gtctggatca ccgccatgcg tactggggcc gccaccgcgc tctcggcacg
     2281 ctacttggcc cgatccgact cgtccaccgt cggtgtcctc ggctgtgggg t
//
LOCUS       DAOSPL010000753         2328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45960_length_2328_cov_3.325561, whole genome shotgun sequence.
ACCESSION   DAOSPL010000753 DAOSPL010000000
VERSION     DAOSPL010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45960_length_2328_cov_3.325561"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="PLQ54_13280"
     CDS             <1..1005
                     /locus_tag="PLQ54_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94285.1"
                     /translation="ETGEVHSLRAPEAGPEGGAQVTFRGVQGTYRIVARFVGLPGVPD
                     GAVRHSSLFSVVPPAQIAEFRQAEERAGRIDVAIFEKGAGARSIAGFLNGQSGAMAAE
                     LVADLKPETLSRFDILIITDPEEPQAVFTPDIAEAIQSFMGQMGGGVLLLHDACGLGG
                     TPVLFAPVGSPLKGGARSTARQVVTLETEHPALEGLPRTQPFKPAFENHVMLLRGVEG
                     VPIIGHPDAGTKPDQGTVVVGELDRGRVALCGLAIGQAGTPDQRREAPPSHQEAAILL
                     NLVRWLAGAPAGSTPSPAPQPGGALPQPAPEGPPGMPQNSGGAVGNLPPRPGEAGGNR
                     "
     gene            1002..>2328
                     /locus_tag="PLQ54_13285"
     CDS             1002..>2328
                     /locus_tag="PLQ54_13285"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOS94286.1"
                     /translation="MTGADLLVAALHNCGIDTAFGIPGFHSNPIYDALRRHGAVNHVL
                     VRHEQTAGFAADGYARASGRIACAITTAGPGATNLATPLAEALSDAIPLLAVTADVDP
                     ESAGRGAYHELAKFALLRPAVKWAARARQPDDIPELVARAIHAALSGRPGPVHLEVPE
                     PVLRAEGSDSPHVRVVPPRRRATSECRAHRAVDVLCEARFPVILAGGGVISAGAEHPL
                     QILAETLHAPVLTTMMGRGALPDRHELAGGFARSPLARRILEHADAMLAIGCRFSEVT
                     TGGWSMRVPRPLIHVDIDPRAFDCRYRADLQIPGDARRVVDQLIGRLARRPAAPPERL
                     ESLARIRREVMEAPAKPDDERSAFAAALRAAVPDDGIVVSDVLHWWDALYSRFPVYSS
                     RTLLVPALFIAMGYALPAAIGAAAACPGRPVVAVMGDGGFFISGMELATA"
BASE COUNT          324 a          877 c          763 g          364 t
ORIGIN      
        1 gagacgggtg aggtccacag ccttcgtgcc cccgaggccg ggcctgaggg cggcgctcag
       61 gtgactttcc gcggtgtcca gggtacgtac cgcatcgtgg cccgattcgt cggtctcccg
      121 ggtgtgcctg atggcgccgt ccgtcacagc tccctcttct cggtggtacc ccccgcgcag
      181 atcgccgagt ttcggcaagc cgaggagcgc gccggccgga tcgacgtggc aatcttcgaa
      241 aagggcgccg gcgcgcgttc gattgcaggc ttcctcaacg ggcagtcggg tgccatggcc
      301 gccgagttgg tggcggacct caagcccgag accctctcgc ggttcgacat attgatcatc
      361 accgaccccg aggagcccca ggccgtgttc actcccgaca tcgcggaagc cattcagagc
      421 ttcatgggtc agatgggtgg tggagtcctc ctgctacacg acgcgtgcgg gctcggcggc
      481 acaccggttc tcttcgcacc ggtcgggtcg cctctgaagg gcggcgcacg gtccacagcg
      541 cggcaagtcg tcacgctcga gaccgagcac cctgccctgg aagggctgcc gcgcactcag
      601 cccttcaagc cggcgttcga gaaccacgtg atgctcctca gaggcgtgga aggcgtcccc
      661 atcattgggc atccggacgc gggcaccaag cccgaccagg gcaccgtcgt ggtgggcgag
      721 ctcgatcgcg gccgggtggc cctttgcggc cttgccatcg gacaggcggg cacccccgat
      781 cagcggcgcg aggcgccccc aagccaccag gaggccgcca tcctgctgaa cctcgtccgc
      841 tggcttgcgg gcgccccagc agggtccact ccctcgccgg cgccccagcc cggcggcgcc
      901 ctgccgcaac ccgcgccgga gggacccccc gggatgccgc agaacagcgg cggcgccgtg
      961 ggcaacctcc cgccgcgccc cggcgaagct ggagggaacc gatgaccggt gccgatctgc
     1021 tcgtggccgc cctacacaac tgtggcatcg acacggcctt cgggattccg gggttccact
     1081 ccaatcccat ctacgatgcg ctacgccgcc acggtgctgt caaccatgtg ctggtgcgac
     1141 acgaacagac agccggcttc gctgccgacg gctacgcgag ggcgtcgggc cgcatcgcct
     1201 gtgccatcac gaccgcgggg cccggagcga cgaatctcgc gacacccctg gccgaggccc
     1261 tatcggacgc catacccttg ctggccgtca ccgccgatgt cgacccggag tctgcgggcc
     1321 gtggcgccta ccacgaactg gccaagttcg ccctgctccg gcccgctgtc aaatgggccg
     1381 ctcgtgcccg gcaacctgac gatatccccg aactggtggc acgcgccatc cacgcggcac
     1441 tctccggccg tccgggcccc gtccaccttg aggtgcccga acccgtactc cgcgccgagg
     1501 gatccgattc cccccacgtg cgggtcgtgc ccccccgccg ccgggcgacg tccgagtgtc
     1561 gagcgcaccg ggccgttgac gtactgtgcg aggcccgctt tcccgtgatc ctcgccggcg
     1621 gtggagtcat ctctgccggc gccgaacacc cactgcagat cctcgccgag accctccacg
     1681 cccccgtgct cacgaccatg atgggccgcg gggcgctgcc ggatcgacac gagctcgctg
     1741 ggggctttgc ccgctcgccc ctggcgcgcc ggatcctcga acacgccgat gccatgctcg
     1801 ccatcgggtg ccggttctcc gaggtgacca ctggcggctg gtcgatgcgg gttccaaggc
     1861 ccctcatcca tgtcgacatc gacccccgcg ctttcgactg ccgctatcgc gcagatctgc
     1921 agatcccggg cgatgcccga cgtgtggtcg accagctcat cggtcgcctg gcccgccggc
     1981 cggccgcccc acccgagcgg ctcgagagcc tggcccgcat acggcgcgag gtcatggagg
     2041 cccctgccaa gccggacgac gaacgaagcg ccttcgccgc cgcgctgcgc gccgcggtac
     2101 ccgatgacgg catcgtcgtg tccgatgtac tgcactggtg ggacgcgctg tactcgcggt
     2161 tccccgtcta ctcgtcccgg acgctcctgg tgccggctct gttcatcgcc atgggctatg
     2221 cgctcccagc tgcgatcggc gcggccgccg cgtgcccggg ccgccccgta gtggccgtca
     2281 tgggcgatgg gggcttcttc atatcgggca tggagctcgc gacggcgg
//
LOCUS       DAOSPL010000754         2328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45961_length_2328_cov_3.324241, whole genome shotgun sequence.
ACCESSION   DAOSPL010000754 DAOSPL010000000
VERSION     DAOSPL010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45961_length_2328_cov_3.324241"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="PLQ54_13290"
     CDS             <1..1208
                     /locus_tag="PLQ54_13290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS94287.1"
                     /translation="GRIGSHHPPDGPPYSARPNTFPAPPCYNPAMRSQPTVLNIAPPA
                     SGTGGIAFLADLEQRSALAERYRLIRFPLARRDEPDRTPWHRLVRTVDRGGALVAALL
                     RHRPDLAQIHTASRGSFWSNAAAGRICLRLGCPFVFRIHGGAFADFLEELSPEGRARA
                     RAALRSARAVAFLSDRWAERIQELVGELNWTVIPNAVEFPMIPAEERPARAPGAPVTF
                     LFIGTLRPLKGLYELIDAARILRDRGGGPPVRFLIVGGGRNDEEAAIRAHYAADPTGT
                     VEFAGIRRGEEKIDAFRQADVLLLPSHTEGQPLVILEAFAAGLPVIATRVGAIPEMIG
                     EGIHGFLIPPRDPEAIAQAVVRLAADPSLRQRMGETNQALFREQYTLERYVERVDRHW
                     KQALRRDPL"
     gene            complement(1228..>2328)
                     /locus_tag="PLQ54_13295"
     CDS             complement(1228..>2328)
                     /locus_tag="PLQ54_13295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS94288.1"
                     /translation="RYQPALECFKKHFDVRDAFSITLPKWQLALLAGRALFCNSAVRN
                     NAIHGGPRVMRAKSRACEARLRPILDEIDAVLLLGPLFCPFAHLPPPKPYFVWTDAMA
                     VKDQKESYSWWTPSWQHRPRWAFQKEIAQNALRVFPFSEWSRQRVLRSFDLPEERVIR
                     SGWTALLPVPDEPVEKNLDEPLVLFAGSNYVRKGAHTLARAVPLVRRHVPNCRFVIAG
                     NPGTAGPPLEPVEGLEILGPVSDRQQMMDLFRRATVFCLLQEYEPAGHVWFEAMGYST
                     PCVGTNICAAPEVILEGQTGYLVPVGDHEAAADRIVRLLTNPAEYYRMSKEARALYER
                     EGRWDQIVGRMADHMKAALEEHGASGRTQESP"
BASE COUNT          376 a          769 c          778 g          405 t
ORIGIN      
        1 cgggccggat agggtcccac catcctccgg atggtcctcc ctattcggcc cggccgaaca
       61 ccttccccgc gcctccttgc tacaatccgg ccatgagatc gcagccgacc gtcctcaaca
      121 tcgccccgcc cgcctcgggc acggggggca tcgcgttcct ggccgatctc gagcagcgtt
      181 cggccctggc ggagcggtac cggctgatcc gcttccccct cgcccggcgc gacgagcccg
      241 accgcacccc atggcatcgt ctcgtccgca ccgtcgatcg tggcggagcg ctcgtcgccg
      301 ccctgctgag gcaccgcccc gatctcgccc agatccacac cgcctcgcgg gggagttttt
      361 ggtccaacgc tgccgcgggg cggatctgcc ttcggctggg ttgccccttc gtgttccgca
      421 tccatggggg cgccttcgcc gatttcctcg aagagctctc accggagggg cgagcacgag
      481 ccagggccgc gctccgcagc gcgcgggcgg tggcattcct ctcggatcga tgggccgagc
      541 ggatccagga gttggtgggg gagctgaact ggaccgtcat cccgaacgcg gtggagttcc
      601 ccatgatccc ggccgaggag cgacccgcgc gtgctcccgg ggcgccggtt accttcctgt
      661 tcatcgggac actgcgccca ctgaaggggc tctatgagct catcgacgcg gcacggattc
      721 tgcgcgatcg tgggggaggg ccacccgtgc ggttcctcat cgttggggga ggacggaatg
      781 acgaggaggc cgccatccgc gctcactatg cagcggatcc tacgggcacg gtagagttcg
      841 cgggcatccg gcgtggggag gagaagatcg atgcctttcg tcaggccgat gtgctcctgt
      901 tgccttccca taccgagggc cagcccctgg tgatcctcga ggccttcgcg gccggtctgc
      961 cggtcattgc cacccgggtg ggcgcgatcc cggagatgat cggggagggg attcacggtt
     1021 tcctgatccc cccgcgggat ccggaagcca tcgctcaggc ggtggtccga ctggcggctg
     1081 accccagtct tcgccagcgc atgggagaga cgaatcaagc gctattccgc gagcagtaca
     1141 cgctcgaacg ctacgtggag cgggtggacc gccactggaa gcaggcgctc cgtcgagatc
     1201 cgctctgagg tcccaagctt ccggggctca cgggctctct tgcgtccgcc cgctggcgcc
     1261 gtgttcctcc agcgcggctt tcatgtgatc ggccatccgg cccacgatct gatcccaccg
     1321 gccctctcgc tcgtacaagg ctcgggcctc tttagacatg cgatagtact cggccggatt
     1381 ggtgagcaga cgaacgatgc ggtcggccgc cgcctcatgg tcccccacgg ggacgagata
     1441 gccagtctgc ccttcgagga tgacctctgg tgccgcgcag atattggtcc ccacgcaggg
     1501 cgtgctgtaa cccatggctt cgaaccacac atgcccggcg ggctcgtact cctggagcag
     1561 gcaaaagacg gtggctcgcc ggaacaggtc catcatctgc tggcggtcgg agaccggccc
     1621 gaggatctcg agtccctcaa ccggttccag tggaggcccg gcggtaccgg ggttacccgc
     1681 aatgacgaac cggcagttcg gcacatggcg gcgcaccaga ggaactgccc tcgcgagggt
     1741 gtgagcccct ttgcggacat agttgctgcc cgcgaacagc acgaggggct catccaggtt
     1801 cttctccacg ggctcatcgg ggacggggag aagggccgtc catcccgacc ggatgactct
     1861 ctcttccggc aggtcgaagg agcgcagcac tcgctgccgg gaccattcgg agaaggggaa
     1921 gacgcggagc gcgttctggg cgatctcctt ctgaaaggcc cagcggggtc ggtgttgcca
     1981 ggagggagtc caccagctgt agctttcctt ctggtccttg accgccatgg catccgtcca
     2041 gacgaagtag ggcttgggcg gcgggaggtg ggcgaaggga cagaagagcg gtcccaagag
     2101 aagcacggca tcgatttcgt cgagaatcgg cctcagtcga gcctcacagg ctcgcgactt
     2161 ggcgcgcatc acccgcggac caccgtggat ggcattgttt cgcacggccg agttgcagaa
     2221 gagagctcgg ccggccagca aggccagttg ccacttcggc agggtgatgg agaaggcgtc
     2281 gcgcacatca aagtgtttct tgaaacattc gagggccggc tgataacg
//
LOCUS       DAOSPL010000755         2328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45968_length_2328_cov_2.520458, whole genome shotgun sequence.
ACCESSION   DAOSPL010000755 DAOSPL010000000
VERSION     DAOSPL010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45968_length_2328_cov_2.520458"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1770
                     /locus_tag="PLQ54_13300"
     CDS             <1..1770
                     /locus_tag="PLQ54_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94289.1"
                     /translation="EDQRQRDEETQLIAGLPIIQPEVTFTPGPLAPSQWPDDETTVVL
                     RRSAFMAPGEGLGDCTPVYEPIEDGGFRIAGSRRQLNRPIVHGMNRLWTGDVPLFRMD
                     SVMGCGAYSTDRVYPLWPRPRASEGGTYPAMGTLRLGVPLGDGRTVWLDELTDTVTEF
                     RPGYTDYTVRAPDDCWRATIWVAPTLSFHGMVCRVEFGEPTRLQWRFGGIWWDPTEAN
                     ANRAEMTGGAVRITEANLPNGLVLAGWDTPSEGRITQESYGQGAEFTASEPQRIYHIV
                     ATWGITEHDHELAEQVMARLDTPNASGWANERDRLKRLWYDCYMAPALEPAAHFASLR
                     RSAEAELLRTRQHWDARRREFHVHTPDEHLNALVNWSRCVSEYHRYGPGLVLGAHFWH
                     MYSHISTGWKGKQWGGDHQALAECLRLYGAMQADDGVIRWVSPSLAPFVAEDNTPYWV
                     DQVWWHYAWTGDAQFVRDLWPAVKRAVACECRQNDPDGDGLFRSWYEYWNCDSNGKGP
                     KAAAPSATGWAMLDCAARLAVVAGDDAAAQSMRAKPNAPGSGSSSSCGETTGAGWGRS
                     APMASGAPRDRLGSSTSRSTRVY"
     gene            1794..>2328
                     /locus_tag="PLQ54_13305"
     CDS             1794..>2328
                     /locus_tag="PLQ54_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94290.1"
                     /translation="MRWIERHYGFEPNPGVNLLAVSGWFPIRWSTQWVPTGDTCLAAL
                     AGIRCGDADLWWPYLRTAVLSSFRSDFPGINMGIANTGAGGGDREDVDSDDPFTHVVV
                     RGLFGIEPALHEARIDITPGLPSDWTAASIRTPDVEYEYRRDGDHATLHIRTPQPVAK
                     RVRVGVDGETVTTPAEAE"
BASE COUNT          387 a          766 c          827 g          348 t
ORIGIN      
        1 gaggaccagc gccagcggga cgaagagacc caactcatcg ccggactgcc gatcatccag
       61 cccgaagtga cgttcactcc cgggcccctg gcgccgagtc agtggccaga cgacgagacc
      121 acggtcgtgc tccggcgatc ggccttcatg gcgcccggtg agggcctggg cgactgcacg
      181 ccggtctacg agcccatcga agacggcgga ttccgaatcg ccggcagccg gcgacagctc
      241 aaccggccca tcgtccacgg catgaaccgc ctatggaccg gtgacgtgcc cctgtttcgc
      301 atggactcgg tgatgggctg cggcgcgtat tcgacggacc gggtgtaccc cctgtggccg
      361 cgccccaggg cctcggaggg cggcacctat ccggccatgg ggacgctgcg gctgggcgtt
      421 ccgctcggcg acggccgaac ggtgtggctc gacgagctga cggacacggt cacggagttc
      481 cggccgggct atactgacta cacggtgcgc gcgccggacg attgctggcg ggcgacgatc
      541 tgggtggcac cgaccctgag cttccacggc atggtgtgcc gcgtcgagtt cggtgagccc
      601 acgcgtctgc agtggcggtt tggcggtatc tggtgggatc cgaccgaggc caacgcgaac
      661 cgggcggaga tgaccggcgg cgccgtgagg atcacggagg cgaacctccc caacggactg
      721 gtgttggccg gatgggacac cccgagcgag gggcgcatca cacaagagag ctacggtcag
      781 ggagccgagt tcacggcgtc ggagccacag cggatctacc acatcgtcgc gacctggggc
      841 atcaccgagc atgatcacga gctcgccgaa caggtcatgg cacgtctcga cacgccgaac
      901 gcttccggtt gggcaaacga gcgcgaccgg ctcaagcggt tgtggtacga ctgctacatg
      961 gcgcccgctc tcgagcccgc ggcgcacttc gcgtcgctcc ggcggtccgc cgaggccgag
     1021 ctcttgcgga cgcggcagca ctgggacgcg cggcgacggg agttccacgt ccacacgccc
     1081 gacgagcacc tcaacgccct ggtgaactgg tctcgctgtg tcagcgagta ccaccgctac
     1141 ggtccgggac tcgtgcttgg tgcccacttc tggcacatgt actcgcatat ctccacgggc
     1201 tggaagggca agcaatgggg cggcgaccat caggcactgg ccgagtgcct gcggctctac
     1261 ggcgcgatgc aggccgacga tggggtcatc cgctgggtgt cgccgtcgct agccccgttc
     1321 gtggccgagg acaacacacc ctactgggtg gaccaggtct ggtggcacta cgcctggacg
     1381 ggcgacgctc agttcgtgcg cgacctctgg ccagcagtga agcgggccgt tgcctgcgag
     1441 tgccggcaga acgatccgga cggcgacggg ctattccgct cgtggtacga gtactggaac
     1501 tgtgactcca acggtaaggg gccgaaggcc gcggcgccga gtgccaccgg ctgggccatg
     1561 ctagactgcg cggcgaggct cgcagttgtg gccggagacg atgccgcggc ccagagtatg
     1621 cgcgccaagc ccaacgctcc agggagcgga tcctcttcga gctgtggcga gacgaccggg
     1681 gccggctggg gtcgatcggc tccgatggca tctggcgcgc ccagggacag acttgggagc
     1741 agtacctcgc gatcaacgcg ggtctactga cgcccgatcg cggccgggcc gccatgcggt
     1801 ggattgagcg gcactacggc ttcgagccca acccgggcgt gaacctgctc gcagtcagcg
     1861 ggtggttccc catccgctgg agcacgcagt gggtacctac cggcgacacc tgcctcgccg
     1921 cgctggcggg gatccggtgc ggtgacgccg acctctggtg gccctacctc cgcaccgcag
     1981 tcctgtcatc gttccgcagt gacttccctg gcatcaacat gggcatcgcc aacaccgggg
     2041 cggggggagg cgaccgggag gatgtggact ccgatgaccc gttcactcat gtcgtggtca
     2101 gggggctgtt cggcatcgag ccggcactgc acgaggcacg gatcgacatc actccgggtc
     2161 tgccgtccga ctggaccgcg gcgagcattc gcacgcccga cgtggagtac gagtaccgcc
     2221 gcgacggcga ccacgcgacg ctgcacatcc ggacgccgca gccagttgcg aagcgggtgc
     2281 gagtgggcgt cgacggagag acggtgacga ctccggcgga ggcagaat
//
LOCUS       DAOSPL010000756         2327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45991_length_2327_cov_3.365317, whole genome shotgun sequence.
ACCESSION   DAOSPL010000756 DAOSPL010000000
VERSION     DAOSPL010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45991_length_2327_cov_3.365317"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            293..1843
                     /locus_tag="PLQ54_13310"
     CDS             293..1843
                     /locus_tag="PLQ54_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94291.1"
                     /translation="MRSRIVPALAWAAAALLLHGSAGAQGRATSVVRSGDLVWADRYS
                     PRFSPDGRALAYVEETHLGYNRSQRAVWVLDLGSMVAGCASREGGGIDPAWSEDGSLI
                     AYIEPGREDPLRGRVYVAERTGSGRRPVTAHEDAFEPTWMFGSGRIVYLRHTAEGGGL
                     RNVVCTIKPGGEDYQEVRSWPIVEQPGDVEWSRSGRFAAYWIRAAATQDVGGAPGRVL
                     TLRLLNLETKEERTLPLPPDTLWVGRMVWSPDDKYIGIEATFDKNRTEIWAIPVAPAG
                     QAAEPRRITAGKEGESFYFPAWSGNGIVCVKYPRPSDSGKAALPELILADFDSRVTYP
                     LKPDWARPVDPSEDIEMVSSPDGRMVVVASGAELRFLELGTEAERNRVLAEDQIRRVA
                     RALVRYSFERNGGVTPSPRNLGGKPEGEFFWVDLIKDLVRPEYLRAPGDTDTKSPSSY
                     TYPKEAYNLDARPLSGDSDRVILIERSGIHAGGHYEAKIGGQVVWVPDATPADAGGPA
                     APEGAPPK"
     gene            1887..>2327
                     /locus_tag="PLQ54_13315"
     CDS             1887..>2327
                     /locus_tag="PLQ54_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94292.1"
                     /translation="MRMLAALTAVSLLVGGLWLLDGVRADRDPEMVMRDQWLGAYLGG
                     NKLGFGHLTTERVRAGGRDMAIVRLTASVRAAAMLGAGAAEIAYRLVQKMDPKTLAPL
                     SVESLMDMNGTVSRTRVKFTADAAEWEILQGGIRRTGSVPLDRGK"
BASE COUNT          384 a          767 c          805 g          371 t
ORIGIN      
        1 ggactgaccg gcgagatcct ggaggtcccg gacctggcgt ccaaagagcc gaagtaaatc
       61 cagccttagg gctgcatccc cttttgccgt tcttgccctt gacaaaacag cttatagtgt
      121 gtataatagt tttgcgatgc tctagtccga caccgcgatc cacctcgctg acctcgcgca
      181 cctgaacaac cccctcagtg ccacgtttcg ccagaggcag gataggcgcg tctcgggcgg
      241 ctggcgaacg ccggcaggcc ctcaggcgtc caagttccgg ggggatactc acatgcgttc
      301 tcgtatcgtt cccgcgctgg cctgggccgc ggcggctctg ttactgcacg gctcggccgg
      361 cgctcagggg cgagctacca gtgtcgtccg tagcggggac ctggtctggg ccgaccgcta
      421 ctcgccgcgc ttctcgcccg acgggcgtgc gctcgcctat gtggaggaga cccacctggg
      481 ctacaaccgg tcgcagcggg ccgtgtgggt gctcgacctg ggcagcatgg tcgccggctg
      541 cgccagccgg gaggggggcg gcattgatcc cgcgtggtct gaggatggct cgctgattgc
      601 ctacatcgag ccgggccgcg aggacccact tcgcggccgg gtctatgtgg cggagcgcac
      661 tggatccggc cgtcggcccg tcacggccca tgaggacgcc ttcgagccga catggatgtt
      721 cggatcgggc cgcatcgtct acctcaggca cacggccgag ggcggcgggc tgcgcaatgt
      781 ggtgtgcacc atcaagccgg gaggcgaaga ctaccaggag gtccgctcgt ggcccatcgt
      841 cgagcagccg ggcgatgtcg aatggtcgcg gtcgggccga ttcgcggcgt actggatccg
      901 cgccgcggcc acgcaggacg tcggcggcgc cccgggccgc gtcctgaccc tgaggctact
      961 gaacctcgag acgaaggaag aacgtaccct cccactgccg ccggacaccc tctgggtcgg
     1021 ccgcatggtg tggtcgcccg atgacaagta cattgggatc gaggcgacct tcgacaagaa
     1081 ccgcaccgag atctgggcca ttccggtcgc accggcaggt caggccgccg aaccgcgccg
     1141 catcaccgcc ggcaaggagg gagagagctt ctacttcccc gcatggagcg gaaacgggat
     1201 cgtgtgcgtc aagtatcccc ggccctccga cagcggcaag gccgctctgc ccgagctgat
     1261 cctcgccgac ttcgacagcc gcgtgaccta tcccctcaag cccgattggg cacgtccggt
     1321 ggacccatca gaggacatcg agatggtctc gtcccccgat ggcagaatgg tggtcgtcgc
     1381 gtcgggggcg gagctccgat tcctcgagct gggcaccgag gccgaacgca accgcgtgct
     1441 ggccgaggac cagatccggc gggtggctcg agcactcgtg cggtactcgt tcgagcgcaa
     1501 cggcggtgtg acgccatcgc cccgtaacct cggtggcaag ccggagggcg agttcttctg
     1561 ggtcgacctg atcaaggacc tggtacggcc cgagtacctg cgcgcgccgg gcgacaccga
     1621 caccaagtcg ccctcgtcct acacgtatcc caaggaagcc tacaacctgg atgcccgtcc
     1681 actctcgggc gacagcgatc gtgtcatcct catcgagcgg tccgggatcc acgccggcgg
     1741 ccattacgag gccaaaatcg gcggccaggt ggtgtgggtg cccgatgcca caccggccga
     1801 tgcggggggg cccgccgccc cggaaggcgc accacctaag taagtcatgg cgaacccagg
     1861 ggaccatcag aatggggcgg atcatcatga gaatgctcgc ggccctgacg gccgtctcgc
     1921 tcctggtggg aggcctgtgg ctcctggatg gcgtccgggc cgaccgggat ccggagatgg
     1981 tgatgcggga ccagtggctc ggggcctacc tgggcgggaa taagctcggc ttcggacacc
     2041 tgaccaccga gcgggtgcgg gccggcggcc gcgatatggc catcgtccgc ctcacggcct
     2101 cggtgcgcgc ggcggccatg ctcggcgcgg gggcggccga gatcgcctat cgcctggtgc
     2161 agaagatgga tccgaagacc ttggccccgc tctccgttga gagtctgatg gatatgaacg
     2221 gcacggtgtc gcgcacccgg gtcaagttca cggccgacgc cgccgagtgg gagatcctgc
     2281 agggcgggat acgacggacg ggctcggtgc cgctcgacag aggcaag
//
LOCUS       DAOSPL010000757         2327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_45999_length_2327_cov_2.764525, whole genome shotgun sequence.
ACCESSION   DAOSPL010000757 DAOSPL010000000
VERSION     DAOSPL010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45999_length_2327_cov_2.764525"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="PLQ54_13320"
     CDS             complement(<1..1094)
                     /locus_tag="PLQ54_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94293.1"
                     /translation="MAASAVTVSLWVVGTTCMLAADEPVWIRPPSVAPSGQPTRFAGA
                     LELTAKPDTALLRIAAYRRYRLIVNGRGVSVGDTPWDAETYDAAPLLHRGRNEIIAEV
                     ADDAAPAPENAWLWLRQQLPQPFRARKLTFETQGAVADEWLYVELLDADGDTTGLFCS
                     ERDHPDLMLGRGGQAARHEIRLDTQDRLAWRATGTCDLDHVVAVRIRLDQKRTSQTLA
                     GSVRIAGVILSGPDGELGLSPSAWSVEAGTGEYRRSTVEPLSDGNGLVLHYDFTPAAS
                     PALAVDLRAWRDGREIGRLVSGAEWSTDSGRATPGWMPPVEWISWVRTSLTGPDEPTS
                     PRRSACTEVRLRSGDRCHVGRGVAGCDIADS"
     gene            complement(1084..2112)
                     /locus_tag="PLQ54_13325"
     CDS             complement(1084..2112)
                     /locus_tag="PLQ54_13325"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS94294.1"
                     /translation="MLTAFEHEEPDRVPAWCGASVEFWEKAKAELGLDDEGLRIRFGD
                     DFRRVRAPFAGPEEPLSPGATYRTVFGIEREGLGYGQPMSHPLAEATLQQIHEYPWPD
                     PAWTDVSHVRAEALAYGRQYAILGGDWSPFFHDAIDLLGMERLYYAMYDEPELVDALM
                     RHMVDYYAGVSTRIFDAAADAIDIFFIGNDLGGQTGPLLGVDLFRRFILPHLARLVDL
                     GHAYGLKVMLHCCGGIAPLIPDLVAAGLDGLHAVQPSCAGMDLRALKAQFGDRILFNG
                     AIDSHHVLINGSPESVREQTRQVLKIMMPGGGYVAGASHDTILEETPVENVVAMFDTV
                     KEYGTYGR"
BASE COUNT          376 a          833 c          777 g          341 t
ORIGIN      
        1 gagtcggcga tgtcgcagcc cgctacaccc ctgccgacgt ggcagcggtc gccggagcga
       61 aggcgcactt cggtgcacgc ggatcgccgc gggctcgtcg gttcgtcggg cccggtcagg
      121 ctcgtccgca cccacgagat ccactccacc gggggcatcc acccgggcgt ggcgcggcca
      181 ctatcggtgg accattcggc cccggagacc agtcggccga tctctcgccc gtctcgccag
      241 gctcggagat cgacggccag cgcggggctg gcggccggag tgaagtcgta gtgcagcacg
      301 aggccgttgc cgtcagacag gggctcgacg gtgctgcggc gatactcccc ggtcccggcc
      361 tcgaccgacc aggccgaggg ggacagtccg agctcaccat cggggcccga gagtatgacg
      421 cccgcgatcc gcacactccc ggccaaggtc tggctggtgc gcttctggtc gagacggatg
      481 cgcacggcga cgacgtggtc gaggtcgcac gtccccgtgg cgcgccacgc caggcggtcc
      541 tgcgtgtcga gccggatctc gtgccgcgcg gcctgcccgc cgcggccaag catcaggtcc
      601 gggtggtccc gctccgagca gaacagcccc gtcgtgtcgc cgtcggcgtc cagcagttcc
      661 acgtagagcc actcgtcagc cacggcgccc tgggtctcga acgtgagctt cctggcccgg
      721 aacggctgcg ggagctgctg ccgcagccac agccaggcgt tctccggggc tggcgcggcg
      781 tcatccgcca cctctgctat gatctcattc cgacctcgat gaagcagcgg agcggcatcg
      841 taggtctccg cgtcccaggg cgtgtcgccc acgctcacgc cacggccgtt cacgatcagg
      901 cggtagcgcc gataggcggc gatgcgcagc aaggcggtgt cgggcttcgc ggtcagctcc
      961 agggcaccgg cgaagcgcgt cggctggccc gagggcgcaa ccgacggcgg gcggatccag
     1021 accggctcgt cggcagcgag catgcatgtc gttcccacaa cccacagtga cactgtcaca
     1081 gcactagcgg ccataggtgc cgtactcctt cacggtgtcg aacatcgcca caacgttctc
     1141 gacgggcgtc tcctcaagga tcgtgtcgtg gctcgctccc gccacgtacc cgccgccggg
     1201 catcatgatc ttcagaactt gccgcgtctg ctctcgaacg gactcggggg agccgttgat
     1261 gagcacatga tgcgagtcga tggcgccgtt gaagaggata cggtcaccga actgcgcctt
     1321 cagcgcgcgc aggtccatcc ctgcacacga gggctgcacc gcatgcagcc cgtccagtcc
     1381 agcagcaacc aagtcgggga tcagcggcgc gatgccgcca cagcagtgca gcatcacctt
     1441 cagaccatag gcatggccca ggtcgaccag gcgggccagg tgcgggagga tgaaccggcg
     1501 gaacaggtct acgccgagca gcggtcccgt ctgcccgccc aggtcattcc cgatgaagaa
     1561 gatgtcgatg gcatcggccg ccgcgtcgaa tatgcgcgtg ctgacgccgg cgtagtagtc
     1621 caccatgtgg cgcatgagcg catcgaccag ctcaggctcg tcgtacatgg cgtagtagag
     1681 ccgctccatg cccagcagat cgatggcgtc gtggaagaag ggggaccagt cgccgccgag
     1741 gatggcgtac tgcctgccgt aggctagggc ctcggcccgc acgtgagaca cgtcggtcca
     1801 cgccgggtcc ggccagggat actcgtggat ctgctgcagc gtcgcctcgg caagcgggtg
     1861 cgacatgggc tggccatagc cgagtccctc ccgttcgatg ccgaagacgg tccggtatgt
     1921 ggcgcccggg gagagcggct cctcggggcc ggcaaatggc gcgcgcacgc ggcggaagtc
     1981 atcgccgaac cgaatgcgca ggccctcgtc gtccagcccc agctcggcct tggccttctc
     2041 ccagaactcc accgacgcgc cgcaccatgc aggcacccgg tcgggctcct cgtgctcgaa
     2101 tgcggtcagc acacgctctc tcgatgtcat cggcacaagc ggggcaaggg gcccccgccg
     2161 tcacctccag cccagaacgg cgctgggcct cagccttccg ccctcgccgc ctcgggccct
     2221 cccgttgcgt ctcccctcgg cagccccacc aacagcgcaa ccgggccggc gatgaggaac
     2281 cacatggcag accccaaacc ccaccgctgg gcggcaactc cgaggcc
//
LOCUS       DAOSPL010000758         2325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46054_length_2325_cov_2.988987, whole genome shotgun sequence.
ACCESSION   DAOSPL010000758 DAOSPL010000000
VERSION     DAOSPL010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46054_length_2325_cov_2.988987"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1049)
                     /locus_tag="PLQ54_13330"
     CDS             complement(63..1049)
                     /locus_tag="PLQ54_13330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HOS94295.1"
                     /translation="MRDARAFWRALVALLVAAAGGCGDTGANSNNNGGGNPLTFSSVD
                     DFAYQLTRANLSELGASAFDLIITDYSLDGSENERLTNEQVTALKSSPGGTKLVLAYM
                     SIGEAEDYRWYWQPAWDANADGEPDAMAPGWLGPANPDWPGNYKVRYWDPDWQGIIFG
                     SAQSYLDKIVAAGFDGVYLDIIDAYEYWGPGGDSGAGRNTAEAEMVAFVKAIAHYARV
                     TLGRAAFGVFPQNGDSLASHADYVAAVTGIGREDLWYLDNSPQPAVAINEALANLDVF
                     RAAGKLVLVTDYVTEPTLIDDFYSKARARGYIPYATVRDLDRLTVHTGHAPD"
     gene            complement(1065..>2325)
                     /locus_tag="PLQ54_13335"
     CDS             complement(1065..>2325)
                     /locus_tag="PLQ54_13335"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOS94296.1"
                     /translation="NRKSIAVRDRLWARAVAIREGETTLVLVSCDIIGVTRSITRHVR
                     ALVGEATGLPPAAIMVHCTHTHSGPATHPIHGWGTPDPPYLSILPHRIARACIEAVGD
                     LSDATLAHAEVPCQGVALNREYDQDAPPLEEVLRDDWRPAKPELVDATCHVLTFRAGQ
                     RLRGFASYYGCHPVVCCETTRYIHGDYAGVATNLLERENPGAVGLFLQGAQGDINSCV
                     VHKPEPEALLALDVIAARYANAVRAGVAAAHPVEVSGLRFALREARFPERPIGLERLR
                     EWLAEQEAVAAAPGASDADREVRMAAVYAESLRGIIPVIESGRPTRPPTELQGLRIGP
                     IAVLGTPFEMFRAIKCEVVEQSSAPVTLVMGLTNDSNGYAADRTTAARGGYAQDMVPL
                     MLGVLPYEDIHTHLVRELLDLESALRS"
BASE COUNT          347 a          775 c          813 g          390 t
ORIGIN      
        1 gccagacgag tgcgacccgt aggtcgccgc caacgggagc gccggtgtgc tcccgagcgt
       61 cctcagtcgg gcgcgtgccc agtgtggacg gtcagccggt ccaggtctcg caccgtggcg
      121 taggggatgt agcctcgtgc ccgagctttg gagtagaagt cgtcgatcag cgtcggctcg
      181 gtgacgtagt cggtgacgag cacgagcttc cccgccgcgc ggaacacgtc caggtttgcc
      241 aatgcctcgt tgatcgcgac ggcgggctgc gggctgttgt cgaggtacca cagatcctcg
      301 cgcccgatac cggtgacggc agccacgtag tcggcgtggg aggcaaggct gtcgccgttc
      361 tgtgggaaca cgccaaacgc ggctctgccc agcgtgacgc gggcatagtg ggcgatggcc
      421 ttcacgaagg ccaccatctc ggcctcggcc gtgttgcggc ccgcgccgct gtctccgccc
      481 ggcccccagt actcgtaggc atcgatgatg tccaggtaga cgccgtcgaa gccggcggca
      541 acgatcttgt cgaggtagct ctgggccgac ccgaagatga tgccctgcca gtcgggatcc
      601 cagtagcgca ccttgtagtt gccgggccag tcagggttcg ccgggccaag ccagccgggc
      661 gccatggcat cgggctcgcc gtcggcgttg gcgtcccagg ccggctgcca gtaccagcga
      721 tagtcctcgg cctcgccgat gctcatgtag gcgagcacga gtttggtccc gcccggactc
      781 gatttcagcg ccgtgacctg ctcgttcgtg agccgctcat tctcactgcc atccagcgag
      841 tagtcggtga tgatcaggtc gaaggccgag gcaccgagtt cgctcaggtt cgcccgggtg
      901 agctgatacg caaagtcgtc gactgaggag aaggtcagcg ggttaccgcc gccattgttg
      961 ttgctgttgg cacccgtgtc gccgcatcca cccgctgccg cgaccagcaa ggccaccagg
     1021 gctcgccaga acgctctcgc gtcgcgcatc gcctgttgcc tcccctacga tcgcagcgcg
     1081 ctctcgagat ccaggagttc gcgcaccagg tgggtgtgga tgtcctcgta tgggagcaca
     1141 cccagcatga gtggcaccat gtcctgtgcg tacccgcccc gggccgccgt cgtccgatcg
     1201 gccgcgtagc cgttggagtc gttggtcagg cccatgacca gcgtgacagg tgccgatgac
     1261 tgctcgacga cttcgcattt gatggcgcgg aacatctcga agggggtgcc gaggaccgcg
     1321 atggggccga tgcgcaaccc ctggagctcg gtcggtggcc gggtcggccg tccggactcg
     1381 atgaccggga tgatgccgcg caggctctcg gcgtacacgg cggccatgcg cacctcgcgg
     1441 tccgcgtccg acgcccccgg tgcggccgcc acggcctcct gctccgccag ccactcgcgc
     1501 agccgctcca ggccaatcgg ccgttcgggg aaccgggcct cgcgcaaggc gaaccgaagg
     1561 ccactgacct cgacggggtg cgccgccgcc actccggcgc gaaccgcgtt cgcgtagcgc
     1621 gcggcgatga cgtccagcgc cagcagggcc tcgggctcgg gcttgtggac cacgcacgag
     1681 ttgatgtcgc cttgcgcgcc ctgcagaaac agcccgacgg cgccgggatt ctcgcgctcg
     1741 agcaggttcg tggcgacgcc ggcgtagtct ccgtggatgt aacgcgtcgt ctcgcagcac
     1801 accaccggat ggcagccgta gtagctagcg aacccgcgga ggcgctggcc tgcacggaac
     1861 gtcagtacgt ggcatgtggc gtcaaccagc tcgggtttgg cggggcgcca gtcgtctcgc
     1921 aggacctctt cgaggggggg agcgtcctga tcgtactcgc ggttcagcgc cacgccctgg
     1981 cagggcacct cggcatgagc gagggtggcg tcgctgaggt cgcccacggc ctcgatacac
     2041 gcccgcgcga tacggtgcgg caggatgctc aggtagggtg ggtccggcgt cccccagcca
     2101 tggatcgggt gcgtggcggg gccgctgtga gtgtgggtgc agtgcaccat gattgcagca
     2161 ggggggagcc ccgtcgcctc gccgacgagg gcgcgcacgt ggcgagtgat tgaccgtgtc
     2221 acgccgatga tgtcacagct caccagaacg agtgtggtct ctccctcgcg gattgccaca
     2281 gctcgcgccc agagccggtc gcgcaccgcg atggacttgc ggttc
//
LOCUS       DAOSPL010000759         2324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46075_length_2324_cov_3.983693, whole genome shotgun sequence.
ACCESSION   DAOSPL010000759 DAOSPL010000000
VERSION     DAOSPL010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46075_length_2324_cov_3.983693"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PLQ54_13340"
     CDS             complement(<1..609)
                     /locus_tag="PLQ54_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOS94297.1"
                     /translation="MRSDRVEQLLAFYRGMLRIRLFEEKILDVMLPGRMFRGSSHLSI
                     GQEAVAVGVVSALGPQDYLITSHRGHGHCLARGMDMEGAFAEIMGRSTGVCAGKGGSM
                     HLSARDLGILGENPVVGSSAPMAAGAALALRMQGSHGVVACFFGEGAINTGAFHEAVN
                     LAALWSLPVVFVCENNRYAISVPVDVASARAKAIHERALGYGI"
     gene            complement(697..1284)
                     /locus_tag="PLQ54_13345"
     CDS             complement(697..1284)
                     /locus_tag="PLQ54_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOS94298.1"
                     /translation="MRPWCLSALSCVCFLLSGCVDIPADIDIPLTIDTDVTGIDTGSS
                     SPGAYIAQLETHAQEKLNALRLAAGVASLPVTRSLGDVARAHSLAMLTGGFFSHEDLQ
                     GRSPQDRLAAARISYDSADELIGFANTQAGVSASDVDAILEELASQRRTEERAILMSP
                     TWTEMGAGIALSDSQRVLFLTIDLVEYPPRVIGVD"
     gene            complement(1302..>2324)
                     /locus_tag="PLQ54_13350"
     CDS             complement(1302..>2324)
                     /locus_tag="PLQ54_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94299.1"
                     /translation="RALGAREVALGCVATHPSLLLSPAVTRDAAMRWVQRQQGFEALV
                     ADVRALWASSPEDFLRGAVAQGLISRAAGLAEGALSARAAAVREAVGNSWDPYVDDLP
                     GAAVEFVNPTMVAYGAEVRDANGDVTGVKLVAGKASEMLLTPWPTWRNPVREPYALGD
                     GHFTIRFTKGYDLTQVGSGSIAYDLESAEGQATIANLMNLATVVTDAFGLFGLDQSSS
                     SWIAAIQATIDKLEDFGAVQGIQASLGQYRLFDAIVGLIQFELASVHLDTLSRWLWDS
                     LPSWLAGDFLAVLQATLGILSTVFESLDFANSDGPFLVDLAFSPWQVTYAVRQTDGVL
                     ASDPDA"
BASE COUNT          421 a          787 c          765 g          351 t
ORIGIN      
        1 gatgccatag cccagagccc gttcgtggat ggccttggcg cgtgccgaag caacgtcgac
       61 cggcacggat atggcgtagc ggttgttctc gcacacgaac accacgggca gactccacag
      121 ggccgccaga ttcacggcct cgtggaacgc tccggtattg atggccccct caccaaagaa
      181 gcaggccaca accccatggg agccctgcat ccggagagcc agagctgctc cggcggccat
      241 gggggcgctc gatccgacga cggggttctc gccgagtatg cccaggtccc gggccgacag
      301 gtgcatgctg ccgcccttgc ccgcgcagac acccgtggag cgccccatga tctcagcaaa
      361 ggcgccctcc atgtccatgc cgcgggcgag gcagtggcca tgcccccgat ggctggtgat
      421 gaggtagtcc tgcggtccca gcgccgacac gaccccgacg gcaaccgcct cctgaccgat
      481 gctcaggtgt gatgaacccc ggaacatcct gccggggagc atcacgtcga ggatcttttc
      541 ctcgaagagg cgtattcgca gcatcccgcg gtagaaggcg aggagctgct ccacccgatc
      601 cgaacgcatg gcgtgacccc ctgggactct gcaccgtgtc gcgtttcgct agcacccgaa
      661 gaagcagcct ccggcgttcc ggggcgagcg cgccggctag tcgacgccga tcacgcgcgg
      721 cgggtactcg acgagatcga tcgtgagaaa cagcacccgc tggctgtcgg acaaggcgat
      781 tccggctccc atttcggtcc aggtcgggct catcagaatc gcccgctcct ccgtgcggcg
      841 ctgggatgcc agttcctcaa ggattgcgtc gacgtcggag gcggagacac ccgcctgggt
      901 gttcgcgaag ccgatgagtt catcggcact gtcgtagctg atgcgagcgg cagcgagccg
      961 gtcctgcggt gatcggcctt gcaggtcttc gtgcgagaag aagcccccgg tcagcatcgc
     1021 caacgagtgc gcccgcgcca cgtcgccgag gcttcgcgtc accggcaggg acgctacgcc
     1081 tgccgcgaga cgcaacgcgt tgagcttctc ctgggcatgg gtctccagct gcgcgatgta
     1141 ggcgccgggg ctgctcgatc cggtgtcgat gccggtcacg tccgtgtcga tagtcagggg
     1201 gatgtcgata tcggccggga tgtcgacaca cccggacaag aggaagcaca cacaggaaag
     1261 ggcgctcagg caccacggcc gcaaggcggg tcctccgttc atcacgcgtc ggggtcagag
     1321 gccagcacgc cgtcggtctg ccggacggcg taagtcacct gccacggact gaaggccagg
     1381 tccaccagga aggggccatc ggagttggcg aagtccagac tctcgaacac cgtcgagagg
     1441 attcccaacg tcgcctggag gaccgccagg aagtccccgg cgagccagga cgggaggctg
     1501 tcccacagcc acctggacag cgtgtcgagg tggacggagg ccagttcgaa ctggatcagg
     1561 cccacgatgg cgtcgaacag ccggtactgc cccaagctgg cctggatgcc ctgaacggca
     1621 ccgaagtcct cgagcttatc gatggtcgcc tggatggccg cgatccaaga tgaggaggac
     1681 tggtcgaggc cgaacagccc aaaggcatca gtcaccacgg tcgccaggtt catcagattg
     1741 gcgatagtcg cctggccctc cgcggattcg aggtcgtagg cgatgctgcc gctgccgact
     1801 tgggtgaggt cgtagccctt ggtgaaacgg atggtgaagt gaccatcgcc cagagcgtat
     1861 ggctcccgaa ccgggttgcg ccaagtcggc cacggtgtga gtagcatctc actggccttg
     1921 cccgcgacca gcttcactcc cgtcacatca ccgttagcgt cacggacctc ggcgccatag
     1981 gccaccatgg tcgggttgac gaactcaacg gcggcgccgg gcagatcgtc aacgtatgga
     2041 tcccacgagt tcccgacggc ctcgcggacg gccgcggcgc gcgccgagag cgcgccctcg
     2101 gccaaaccgg ccgcgcggct gatcagtccc tgagccacgg ccccgcgcag gaagtcctcg
     2161 gggctggacg cccacagcgc cctcacgtcg gcgaccagag cctcgaaccc ctgctgcctc
     2221 tggacccacc gcatcgcggc gtcacgcgtc accgccggcg acaggagcaa cgatgggtgg
     2281 gtcgccacgc accccaatgc gacctcacgc gcgcccaggg cccg
//
LOCUS       DAOSPL010000760         2319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46192_length_2319_cov_3.963339, whole genome shotgun sequence.
ACCESSION   DAOSPL010000760 DAOSPL010000000
VERSION     DAOSPL010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46192_length_2319_cov_3.963339"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PLQ54_13355"
     CDS             complement(<1..353)
                     /locus_tag="PLQ54_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94300.1"
                     /translation="MPEYTGPQHERNAETDRPQVVLSRASTVGPSPEPPKRELMDTLN
                     LAARPALVAMLIVLAAYAAGQATYNLQIQSQPAVNVEGQPTRLPPALHPDAAETAQLA
                     VQIAGFTQCIDDDLT"
     gene            complement(404..1066)
                     /locus_tag="PLQ54_13360"
     CDS             complement(404..1066)
                     /locus_tag="PLQ54_13360"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase-associated EpsI family protein"
                     /protein_id="HOS94301.1"
                     /translation="MRSTLVRLAPATLVALTVVLVARTPGQLGHEFTISEEELPERVA
                     DWTRVPVEGSIPIVSIDGKLQQRKFGYERGDGTRCEIILAVSQNQGSQVFRRFLSSYE
                     VMDLQVGSRRLVDFPAPGGGAGRVNATWVDFVPSGDRSAACAYWYATRGRSVAGYSEQ
                     WRRRILERLMGRQQPWAVVYMLFAAPGDRRPMPNDVATLASAIAGVAEHVLERAPSRL
                     ES"
     gene            complement(1284..2180)
                     /locus_tag="PLQ54_13365"
     CDS             complement(1284..2180)
                     /locus_tag="PLQ54_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94302.1"
                     /translation="MWSRWIGPWMLEALHLGDNAVYLLDGRARWRRFRLWIAPIVCLA
                     LAGGSVLGGLALAPVLATNAEMAVVLRMVLGTTAVAIPGLGDGVAGWQAVMLFGVWSV
                     LGLIGQAVAPGLGAVTIARDAERRALEPVLATPMTDRAIVLGKLAASSYPCFLAALSV
                     LPAFGFCAAVAEIGPRLALFGMASMAVQVFSRSAVGVAFSTLVRRSVAAVVASYAMAF
                     VGIPILQSVVILPLQLLFIHRMGLFTGSMPVSGFSGMMAATRDLTVAATAITAVLDVL
                     IGVLAIGLARRQLSAIRHSGTN"
BASE COUNT          423 a          833 c          743 g          320 t
ORIGIN      
        1 cacgtaagat catcgtcgat gcactgcgtg aatccggcga tctggacggc gagctgagcg
       61 gtctcagcgg cgtcagggtg cagcgccgga ggcaaccgcg tgggctgtcc ctcgacattc
      121 accgctggct gcgactgaat ctgcaggttg taggtggcct gaccggccgc gtaggccgcc
      181 aggacgatca gcatggcgac gagtgccgga cgcgcggcga ggttcagtgt gtccatcagc
      241 tcacgcttcg gcggctctgg gcttggtccg accgtggatg cccgcgagag cactacctgc
      301 ggccgatccg tctcggcatt ccgctcgtgt tgcggaccgg tgtactccgg cacctatgta
      361 ccacctcgca ctccccccgg cttcgaggcg aaccactagc cgcctaagac tccaaccggc
      421 tgggcgccct ctccaagacg tgctctgcca cgcccgcaat ggcggaggcc agagtggcca
      481 cgtcgttcgg catcggccgc cggtctccag gcgcggcgaa cagcatgtac acgaccgccc
      541 agggttgctg gcggcccatc aggcgctcca ggatccggcg acgccattgc tcgctgtacc
      601 ccgcgaccga tcggcctcgc gtcgcgtacc agtaggcaca cgccgccgac cggtcgcccg
      661 acgggacgaa gtccacccag gtcgcgttca cacggcccgc gccaccaccg ggcgcgggaa
      721 agtccaccag acggcgtgac ccgacctgca ggtccatgac ctcgtagctc gataggaacc
      781 ggcggaacac ctgcgacccc tggttctggc tcaccgccaa gatgatctcg caccgcgtcc
      841 catcgccgcg ttcgtacccg aacttgcgtt gctgcagttt gccatcaata ctcacgatcg
      901 ggatcgaacc ctccacggga acgcgggtcc aatccgctac cctctcgggc agctcctcct
      961 cggagatcgt gaactcgtgc ccgagctgcc ccggcgtgcg ggcaaccagc acaaccgtga
     1021 gcgcgacgag cgtcgcgggc gccaaacgga ccagggtgct gcgcattcta tcccccttgg
     1081 gcgccgtgct acccgggcca gcggccgcca cccggcacgc gcttgactgc ctggcatcat
     1141 aaccgacagg gctccgtgac gggacggacc ttcctggaat gccagaagcc actcctgcta
     1201 tcttccatag aacggcgcga tcctacgtga gggtcacctc gagttgtcgg ttgagcggtc
     1261 ggagtgcggc ggcatggagg acgctaattc gtgccggaat ggcggatcgc cgagagctgc
     1321 cgccgggcca aaccgatggc cagcacgccg atgagcacat ccaacacggc cgtgatcgcc
     1381 gtggccgcca ctgtcaggtc gcgtgtcgcc gccatcatgc ccgaaaagcc tgaaacaggc
     1441 atcgagcccg tgaacaaccc catccggtga atgaacagga gctgcagcgg caggatgacc
     1501 accgattgta ggatggggat cccgacgaac gccatggcgt aggatgcaac cacggccgcc
     1561 acggagcgcc gcaccagcgt ggagaaggcc acgcccaccg ccgatcgact gaatacctgc
     1621 acggccatgc tcgccatgcc gaacagcgct agccgtggcc cgatctcggc tacagctgcg
     1681 cagaagccga acgcggggag caccgagagg gccgccagga agcacgggta gctggacgcc
     1741 gcgagcttgc cgaggacgat cgcccggtcc gtcatcggcg tcgcgagcac cggctccagg
     1801 gcgcgccgct cggcatcacg ggctatcgtg accgcgccga ggcccggagc gacggcttgg
     1861 ccaatcagcc ccagaacgct ccagacgccg aacagcatga cggcctgcca gccggcaacg
     1921 ccatccccaa ggcccgggat cgcgaccgcg gtcgtgccca gcaccatgcg gagcaccacg
     1981 gccatctcgg cgttcgttgc caggaccggg gccagggcca ggcctcccag gacggacccg
     2041 ccggcgaggg cgaggcacac gatgggcgcg atccagagtc ggaaacgccg ccagcgcgcg
     2101 cggccgtcca gcagatagac ggcgttatcg cccaagtgca gtgcctcgag catccatggc
     2161 ccgatccagc gcgaccacac ggccgtagcc accatcccta ccgtccgcgc agcctgcggc
     2221 acagatgcga cacgttgcgg aggccgagag ccattgccgc cagcaggaac gccaacccgg
     2281 ccatcctggc cgtggaccac accgggacga tgtgggcct
//
LOCUS       DAOSPL010000761         2315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46311_length_2315_cov_3.524336, whole genome shotgun sequence.
ACCESSION   DAOSPL010000761 DAOSPL010000000
VERSION     DAOSPL010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46311_length_2315_cov_3.524336"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PLQ54_13370"
     CDS             complement(<1..238)
                     /locus_tag="PLQ54_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005991664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOS94303.1"
                     /translation="MSCPSIELNVVAEEYRQAAAGQHRRIIVCAGTGCVANGSLRVRD
                     AFVAQLPEAGWSAITVPSPASEDEADRRVLVSGSG"
     gene            complement(235..714)
                     /locus_tag="PLQ54_13375"
     CDS             complement(235..714)
                     /locus_tag="PLQ54_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOS94304.1"
                     /translation="MVRKFEKVCAILDQHGRDPAKLIPILQEVQEEYRYLPEQVLTFV
                     ATSLGLPPARVYGVATFYAHFALKPKGKHVIRLCDGTACHVKQSIPILEAIRARLGLT
                     ETMDTTPDMLFTVETVSCLGACGLAPVVVVDEDVHGQMTPESAVALVDAIVEAEGSR"
     gene            complement(728..1420)
                     /locus_tag="PLQ54_13380"
     CDS             complement(728..1420)
                     /locus_tag="PLQ54_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOS94305.1"
                     /translation="MMASPKVKPVPEASLRRLPSYLHLLRRLHAGGRELVSCTHIAES
                     LDLNPTQVRKDLAYTGIVGRPRIGYPIRALIASVEHFLGWDNTTDAFLVGAGSLGRAL
                     LGYAGFQRYNLNIVAAFDVDPERIGTHWRGKPILDVDELPDLAGRMHVHLGVLTVPAD
                     AAQDAAHLLVLSGLKAIWNFTGVALEVPREVLVEDVDLGASLAVLSNRLAQRLRSAEE
                     EESERHVGGHGA"
     gene            1622..>2315
                     /locus_tag="PLQ54_13385"
     CDS             1622..>2315
                     /locus_tag="PLQ54_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS94306.1"
                     /translation="MVSHTRDASSAIDEKRIVCTLETHSRPEPAHVRDVLQKARQAHG
                     LEPAEVAVLAAADDPDLMAELFDAARQVKETIYGRRLVLFAPLYISNMCANECLYCAF
                     RARNREVKRRALTQEEIAREVGILVDQGHKRLLVVAGESYPRQGFQYVLDSIATVYSV
                     ARGRGEIRRLNANIAPLTVDEFRQLKAARIGTYQLFQETYHRETYKKMHVAGVKADYD
                     WRVSAMDRAMEAG"
BASE COUNT          430 a          808 c          691 g          386 t
ORIGIN      
        1 acccgcttcc ggaaaccagt accctccggt ccgcctcatc ctcggaggcc ggagacggta
       61 ccgtgatcgc cgaccacccg gcctccggga gctgggccac gaaggcgtcc cggaccctca
      121 gtgatccgtt ggcgacacag ccagtgcccg cgcacacgat gatgcgccgg tgctgccctg
      181 ccgcggcctg gcggtactcc tcggcgacca cgttcagctc gatgctcgga cagctcatcg
      241 cgacccctct gcctccacaa tcgcgtccac caaggccacc gccgactccg gcgtcatctg
      301 gccgtgcaca tcctcatcca ccaccacgac cggcgccaga ccacacgctc cgaggcacga
      361 gaccgtctcc acggtgaaca gcatgtccgg cgtcgtgtcc atcgtctccg tcagacccag
      421 gcgcgcccga atggcctcga ggatcgggat cgactgcttc acatgacatg ccgtgccgtc
      481 acacaagcgg atgacgtgct tccccttggg cttcagggca aagtgggcgt agaaggtggc
      541 gaccccgtac acccgtgccg gagggagtcc cagcgaggtg gctacaaacg tcaggacctg
      601 ctcgggcagg taccggtact cctcctgaac ttcctgcagg atcgggatca gcttggcggg
      661 atcccgccca tgctgatcga ggatcgcgca gaccttctcg aacttcctca ccatggtagg
      721 cgcgctgcta ggcgccgtga ccgccgacat ggcgctcgct ctcctcctcc tccgcggacc
      781 gcagccgctg ggccaggcgg ttcgagagca ccgccaggct cgcgcccagg tccacatcct
      841 ccaccagcac ctcgcggggc acttccagcg caactcccgt gaaattccag atcgctttga
      901 gcccactgag caccagcagg tgggcggcgt cctgtgccgc atccgccgga accgtcagga
      961 cccctagatg cacgtgcatc cgtcccgcca ggtccgggag ctcgtccaca tccagaatgg
     1021 gcttgcccct ccagtgggta ccgatgcgct cggggtccac gtcgaaggcc gccacaatgt
     1081 tcaggttgta ccgctggaag cccgcatacc ccaagagcgc gcgccccagg cttcccgcgc
     1141 ccaccaggaa cgcatcagtc gtattgtccc agcccaggaa gtgctccacg gacgcgatga
     1201 gcgcacggat ggggtagccg atccgcggtc ggccgacgat gcccgtatag gccaggtcct
     1261 tgcgcacctg tgtcgggttc aggtcaaggg actcggcgat atgcgtgcag gagaccagtt
     1321 cgcgcccgcc ggcgtgcaag cgccgcagga ggtgtaggta agagggcaac cggcgtaggc
     1381 tcgcctcggg gacgggcttg acctttgggc tagccatcat ctcgattcct ccagctctct
     1441 tgcgaactca ctggcgagga taccatatcg accacccaat atcaataccg ttttctcgat
     1501 atcgtgatac cgatatactt ttcctgaatg tcggaacgat tccccagccc gaaacgctct
     1561 acactgaggt caggccgccc tgtgcgcagt cgcctgcagc ccgagagaag gatgcacgag
     1621 catggtcagc cacacccgag atgcctcatc cgccatcgac gagaaacgca tcgtgtgcac
     1681 gctcgaaacc cacagtagac ccgaacccgc tcacgtgcgc gatgtactcc agaaggcccg
     1741 gcaagcccac gggctggaac cggcagaggt ggcggtcttg gccgccgccg atgaccccga
     1801 tctcatggcc gagctcttcg atgcggcccg tcaggtgaag gagaccatct acggccgccg
     1861 actcgtgttg ttcgcgcccc tgtatatctc caacatgtgc gccaacgaat gcctctactg
     1921 cgctttccgc gctcgcaacc gcgaggtgaa gcgccgggcg ctgacgcagg aggagatcgc
     1981 acgcgaggtc ggcatcctgg tcgaccaagg gcacaagcgc ctgttggtcg tcgccggtga
     2041 gtcgtacccg cgccagggtt tccagtacgt cttggattcc attgccacgg tgtacagcgt
     2101 ggctcggggc cgaggcgaga tccgcagact caacgcgaac atcgcgcccc tgaccgtcga
     2161 cgagttccgg cagctcaagg cggccaggat cggcacctac cagctcttcc aggagacgta
     2221 ccatcgtgag acctacaaga agatgcatgt cgctggcgtc aaggccgact atgactggcg
     2281 agtgtcggcc atggaccgag ccatggaggc cggta
//
LOCUS       DAOSPL010000762         2315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46325_length_2315_cov_2.695133, whole genome shotgun sequence.
ACCESSION   DAOSPL010000762 DAOSPL010000000
VERSION     DAOSPL010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46325_length_2315_cov_2.695133"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..1080)
                     /gene="modA"
                     /locus_tag="PLQ54_13390"
     CDS             complement(205..1080)
                     /gene="modA"
                     /locus_tag="PLQ54_13390"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOS94307.1"
                     /translation="MCHDRFRWAAAIVMCSGLVASGCTRQSTDDGAAIASTARGTHDS
                     TAPAKGVPTRKLTVFAGSGAMAALKEIGPLYEADSGIEVDITSGGSGSLLTQFVQEQF
                     GDLYIPGSDDFMDKAEAKEAVLADTRTTLVYLVPCICVAQGNPRGVKGLHDLARTDLR
                     VVLGEPKAVCIGDISEAVLREKGLWDKVQPKVASFGSSCEDVLNKLLLGEADVIIGWD
                     SFAKQNPDKVEAIPIEAKSARVRNIPAAVIRWSTQREAAEAFIEFLTSPRAMEIWEAQ
                     GYTTKPPASASSCPS"
     gene            complement(1334..>2315)
                     /gene="glnA"
                     /locus_tag="PLQ54_13395"
     CDS             complement(1334..>2315)
                     /gene="glnA"
                     /locus_tag="PLQ54_13395"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOS94308.1"
                     /translation="AGYYFIDSDEGIWNAGRDEKPNLGYKPRHKSGYFPVPPMDKQQD
                     LRTEITLAMLQAGIDVEVHHHEVATAGQAEFDLRYDTLVRSADKILMYKYVAKNVAVK
                     HGKTVTFMPKPMFGDNGSGMHTHQSIWKGERNVFYDPSGPYAQLSETAMYYIGGLLKH
                     AASILAFAAPTTNSYRRLVPGYEAPVKLAYSQRNRSACVRIPLYSKSEKAKRIEFRPP
                     DPSCNPYLAFSAMLMAGLDGVRNRIHPGEALDKDLYDLPPEEAALVESTPGSLRDVLM
                     ALEKDHAYLLEGDVFTPDLIEMWISWKMKNEVEAIDLRPHPYEFFLYFDI"
BASE COUNT          394 a          688 c          795 g          438 t
ORIGIN      
        1 gacatggctt gctccttcta ggcggacgag gtacgaatca cacccggtcg cgtctggacc
       61 gaatgcgccc ctggtattct cgcttcggtg cccggaatcc tgacagcttc ctgcaagcag
      121 atgctccgag gcgctcggca ggctcatcat gcgctgcggt gcagcgcgcc ctcaagcgcg
      181 gctaccggga tcggcgcgag ccagctacga ggggcaactc gatgcggacg cgggtggctt
      241 ggtggtgtag ccctgagctt cccagatctc catcgccctg ggggaagtca gaaactcgat
      301 gaacgcctcg gccgcctcac gctgcgtgga ccatcggatc acggcggcgg ggatgttgcg
      361 tacgcgggcg ctcttggctt cgatgggtat ggcctcgacc ttgtcgggat tctgcttggc
      421 gaaggagtcc catccgataa tgacgtcggc ctcgccgagc aagagcttgt tgaggacgtc
      481 ttcgcatgag ctgccgaacg acgcgacttt gggctgaacc ttgtcccaca gtcccttctc
      541 tctgagcacg gcttcggaga tgtcaccaat acagacggct ttgggctcgc caagtacaac
      601 tcggaggtcg gtgcgtgcca ggtcgtggag gccctttacc cctctggggt tgccctgggc
      661 gacgcagatg cacgggacca ggtagaccag ggtggtccgg gtatcggcga gtactgcctc
      721 cttggcttcg gccttgtcca tgaaatcgtc ggatccgggg atgtagagat cgccgaactg
      781 ctcttgcacg aactgggtca gcagggaccc cgagcctccc gaggtgatgt cgacctcgat
      841 gccggaatcg gcctcataaa gcgggccgat ctctttcaga gcagccatcg caccggaccc
      901 ggcgaagacc gtcagcttcc tggtcggcac acccttggcc ggagcagtgg agtcgtgggt
      961 cccccgggcg gtggaggcga tggccgcgcc gtcgtcggtc gactgtcggg tgcagccgct
     1021 cgccaccagg cccgaacaca tgacgatggc cgcggcccag cggaaccgat catggcacat
     1081 aatgagaccc ctccatacga cgagcgtggg agcgacggaa gcctgccggc gagctcgcgc
     1141 ggtgtgcccc cggcggctcc agcagtcctt tcgctcccgc ctccagcact atgacagggt
     1201 ctgcgcggcg tgcgcatggg ggtcgccgag acgctcggag gcccgcacgg gtcacgacca
     1261 gcgcgggcct ccgcagcaga ggcatcgggc cactcaatgc cgtacatggg caccggcggc
     1321 ccggcacggg gggctatatg tcgaagtaca ggaagaactc gtagggatgc ggtcgcaggt
     1381 cgatggcctc aacctcattc ttcattttcc acgagatcca catctcgatg agatcgggtg
     1441 tgaagacatc gccttcgagc aagtaggcat ggtccttctc gagagccatg agcacatcac
     1501 gcaagctgcc gggcgtggac tcgaccagag cggcttcctc gggcggcagg tcgtagaggt
     1561 ccttgtccag ggcctctccc gggtggatgc ggttgcggac gccgtcgagg ccagccatga
     1621 gcatggcgga gaaggcgaga taagggttgc agctcggatc gggcggacgg aactcgattc
     1681 gcttggcctt ctcgctcttg gagtaaagcg ggatccgcac acatgcgctt cggttccgct
     1741 gggagtaggc cagcttgacg ggagcttcgt acccaggaac gaggcggcgg tacgagttgg
     1801 tggtgggcgc cgcgaaagcg agaatcgagg ccgcgtgctt cagcagacca ccgatatagt
     1861 acatggcggt ctctgagagt tgggcgtagg gccccgaggg atcgtagaag acgttccgct
     1921 cgcccttcca gatgctctgg tgggtgtgca tgcccgagcc gttgtccccg aacatgggct
     1981 tgggcatgaa ggtgacggtc ttgccgtgct tgacggccac gttcttggcc acgtacttgt
     2041 acatgaggat cttgtcagcg ctgcgaacca gcgtgtcgta gcggaggtcg aactcggcct
     2101 ggccggctgt ggcgacctcg tggtggtgga cctcgacgtc gatgccggcc tggagcatgg
     2161 cgagggtgat ctcggtgcgg aggtcctgtt gcttgtccat gggcggaacg gggaagtagc
     2221 cactcttgtg gcggggcttg taaccgaggt tgggtttctc gtcgcgaccc gcattccaga
     2281 tgccctcgtc ggagtcgatg aagtagtagc cggcg
//
LOCUS       DAOSPL010000763         2314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46355_length_2314_cov_2.078353, whole genome shotgun sequence.
ACCESSION   DAOSPL010000763 DAOSPL010000000
VERSION     DAOSPL010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46355_length_2314_cov_2.078353"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PLQ54_13400"
     CDS             complement(<1..573)
                     /locus_tag="PLQ54_13400"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94309.1"
                     /translation="MRPELLLTVALTALLLAVVAGRAAGGDYGGAVRRTDGLVAYWPF
                     DGGLEDVVGGALGTPEGMVRFVPGVAGTSGIELDGRGFVTAPPILGADGPESTIELFF
                     RPLPSPSPGNPCLVSIRDEKRARVSLHISADLRRLAVWNGGGAGWVDLAPEADLGEGV
                     WHHVALTHGREGVRLYLDGVAWPGAVGPSGE"
     gene            984..1997
                     /locus_tag="PLQ54_13405"
     CDS             984..1997
                     /locus_tag="PLQ54_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94310.1"
                     /translation="MMSRRLFLCCLAAIAAVCGAALSDDLPATQVDQPTQATGAGETE
                     KGTIAINFAGGTVKDLATYLQEKFDVAMAVAEPMDFAVGPLQFTAPNVDQVYVLLGGM
                     GRVARRPGYLITPKDPTGKEAQPTDLKRPDRPADKVRDESTVGPIKADGVVLTEALGE
                     LATLYRVPFIYEGTPSAEAFAVDAPKCSVRELIEALASHAGCVAKPVYVLYGMTPDNV
                     AQVLDYTSDEEILEGFEKNFGMYANLPKELRMQIFAFAFGELQNMSPEDRAQAIQYAQ
                     DMFGTMFDRLASMQGAGADGVRQKVAGVIDDGLEFYRGLSAGQRGQVQPLAEYFTRMR
                     QKF"
     gene            complement(2004..>2314)
                     /locus_tag="PLQ54_13410"
     CDS             complement(2004..>2314)
                     /locus_tag="PLQ54_13410"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOS94311.1"
                     /translation="ATVMRLTPELAEGFYAEHRGKPFFGGLIDFMTSGICMPMVLEGE
                     DAIERVRAIHGATDPADAAPSTIRARFGTQVTRNAVHGSDSLASAAREVAFFFPQLGA
                     "
BASE COUNT          402 a          793 c          753 g          366 t
ORIGIN      
        1 ctcgcccgac gggccgaccg cccccggcca cgccacgccg tccaggtaga ggcgaacgcc
       61 ctcgcgcccg tgggtcaggg cgacgtggtg ccagacaccc tctcccaggt ccgcttccgg
      121 cgccagatcc acccagccgg ctcctccgcc gttccagacc gccagccgcc tcagatcagc
      181 ggagatgtgc agactgaccc gggcccgctt ctcgtcccga atcgagacca gacacgggtt
      241 gcccgggctc ggtgacggca ggggacggaa gaacagctcg atcgtcgact cggggccgtc
      301 cgcgcccagg atcggtggag ccgtcacgaa accccggccg tcgagctcga tcccggatgt
      361 gcccgccacg cccgggacga agcggaccat gccctcaggc gtccccagcg cgcccccgac
      421 cacgtcttcc agtccgccat cgaagggcca gtaggccaca agcccatcag tccgccgcac
      481 ggcgccacca tagtcaccac cggctgccct accggccacc acggccagta acagggcggt
      541 cagcgcgacg gtcagcagga gttcgggacg catactctca ccccattagc atcagagcac
      601 cgttccgcct attcgcggcc ggcgcgcccg ccccttgggt cgcattcgct cgccgaagca
      661 gagtgctgtg agtccaggcc cggtgcaaat ccactctggg gtccccggcg ctcagccccg
      721 ccagagccgt cgcatggccc ggcagcacgg gtgcccattg gcggctcggc tgtccggaag
      781 ggaaactcag tacaaggagg cgctgactgg tgtgagtacg cgagccacgc cgagcgtcga
      841 agtacccagg aacgcgagct gcatcccatg tctgcgccgg ccgacggcag ggcggatgcg
      901 cggtttgtgt ctgggccgac cgggcatggt tgcgtttcga gacatggtga tcgtagaccc
      961 atggtgaccc acggaaggtg acgatgatgt ccaggcgtct gttcctctgc tgccttgccg
     1021 ccatcgccgc agtttgcggc gccgccctat cggacgatct cccggccacc caagtagacc
     1081 agcccacgca ggccaccggc gccggcgaaa cggagaaggg cacgatcgcc atcaacttcg
     1141 ccggtggtac tgtgaaggat ctggcgacgt acctccagga gaagttcgac gtggccatgg
     1201 ccgtggctga gccgatggac ttcgcggtgg gcccgctcca gttcacggcc ccgaacgtcg
     1261 accaggtata cgtcctactg ggtggcatgg gccgcgtggc tcgccgaccc ggttacctca
     1321 tcacccccaa ggaccccacc ggcaaggagg cccagccgac ggacctcaaa cgtcctgatc
     1381 ggcccgcgga caaggtccgc gacgagtcta ccgtaggccc gatcaaggcc gacggtgtgg
     1441 tcctcaccga agccctcggc gagctcgcca cgctgtaccg cgttcccttc atttatgagg
     1501 gcacgccatc ggccgaggcc ttcgcggtgg atgctcccaa gtgctcagtg cgcgagctga
     1561 tcgaggcgct cgcctcgcac gccgggtgtg tcgccaaacc cgtgtatgtg ttgtatggca
     1621 tgacgcccga caatgtcgcc caggtgctcg actacaccag cgatgaagag attctcgagg
     1681 ggttcgagaa gaactttggg atgtacgcga acctccccaa ggaactgagg atgcagatct
     1741 tcgctttcgc atttggggag ctccagaaca tgtcgcctga agaccgggca caggccattc
     1801 agtacgccca ggacatgttc ggcaccatgt tcgaccggct ggcgtccatg cagggcgccg
     1861 gggcggacgg cgtccgacag aaggtcgcgg gcgttatcga cgacggcctg gagttctacc
     1921 gggggctgtc ggccggccag cgcggccagg tccagccgtt ggctgagtat ttcacccgga
     1981 tgcggcagaa gttctaggtc gatctacgcg cccagttgcg ggaagaagaa ggcgacttcc
     2041 cgggcggcac tggcgagact atctgagccg tggactgcgt tgcgggtcac ttgggtgccg
     2101 aatcgagccc gaatggtgct cggtgccgcg tcggccgggt cggtagcccc gtggatggcg
     2161 cgcacgcgct cgatggcgtc ctctccctcg agcaccatgg gcatgcagat gcctgaggtc
     2221 atgaagtcga tcagaccccc gaagaacggc tttccccggt gctcggcata gaacccttcg
     2281 gcaagctccg gggtgaggcg catcacggtc gcgg
//
LOCUS       DAOSPL010000764         2313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46374_length_2313_cov_3.246236, whole genome shotgun sequence.
ACCESSION   DAOSPL010000764 DAOSPL010000000
VERSION     DAOSPL010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46374_length_2313_cov_3.246236"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PLQ54_13415"
     CDS             complement(<1..880)
                     /locus_tag="PLQ54_13415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOS94312.1"
                     /translation="MPRKPAQDPFLAHALRMKRQLVQWRRQIHQHPELAYGETQTAAL
                     VSGHLRSLGIHVRAGVGGTGVVGLIRGPRLAPTVLLRADMDALPIQEAADVPYRSKVA
                     GVMHACGHDMHTAMLMGVASLLAARAGELAGSVKLCFQPAEESGAGALAMIRDGVLAD
                     PPIDAAVALHVHSRLPEGVVGLLTGPVTAFADSLRIIVRGRSAHAAAPHEGIDTIVVA
                     AHLITSLQSLVAREIDPMRQRVVSLGMIGGGTAPNVVAESVTIDGTIRSFEEPVRRRL
                     FARVRRHAKAVAQSMGA"
     gene            complement(907..1635)
                     /locus_tag="PLQ54_13420"
     CDS             complement(907..1635)
                     /locus_tag="PLQ54_13420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOS94313.1"
                     /translation="MRPLEGKIALVTGAGRGIGAAVASALAEAGATVVVTSRTPMELA
                     TVVDAIRSGGGCALAIEGDITDEAFQDRLFAEIDRIFSRLDILVNNAGIAPFGPVEEL
                     PASDLRRCLELNVVAAYGCMQRAIRLMRDNGAVGKIINIGSVRSHWTEAGDAGAYNAS
                     KEGLRAMTQSVARQLHGTGVRIAVGMVNPGVVDTSLTNPSGTPKPDWLKPETVARAVL
                     HAVTAPPDVNVFDTTLFNVSQSPW"
     gene            1883..2086
                     /locus_tag="PLQ54_13425"
     CDS             1883..2086
                     /locus_tag="PLQ54_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94314.1"
                     /translation="MASTPIDLLPNVNAVCEELRRRMAGMSAEQKAAFLLASARLAAA
                     QADSLSSDGRHRTEVVVDQCGSC"
     gene            complement(2083..>2313)
                     /locus_tag="PLQ54_13430"
     CDS             complement(2083..>2313)
                     /locus_tag="PLQ54_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS94315.1"
                     /translation="RLVLLDMRMPRMSGEEALDAIRQRRPDVPIIVASGYSEQDATRH
                     LDGLGRAWFIRKPFRLADLETLIKEALAAAES"
BASE COUNT          368 a          762 c          808 g          375 t
ORIGIN      
        1 tggcgcccat ggattgggcc acggccttgg catgccggcg aacccgcgcg aatagccgcc
       61 ggcggaccgg ctcctcgaag gaccggatcg tgccgtcgat cgtgaccgat tcggccacga
      121 cgttgggtgc ggtgccgccc ccgatcatcc cgagcgagac gacgcgctgc cgcatgggat
      181 cgatctcgcg tgccaccagg ctttggagcg aggtgatgag atgcgcggcc acgacgatgg
      241 tgtcgatgcc ttcgtgaggc gccgcggcgt gggcactccg gccgcgcacg atgatcctca
      301 ggctgtcagc aaaggcggtc actgggcccg tcaggaggcc gacgacccct tcgggcaggc
      361 gggagtgcac gtgcagtgcg acggcggcat caatgggcgg gtcggcgagc actccatcgc
      421 ggatcattgc cagggctccc gccccggact cctcggccgg ctggaagcac agtttcacac
      481 ttcccgcaag ctcgccggca cgggccgcca gcagcgaggc cacgcccatc agcatggccg
      541 tgtgcatgtc gtgcccgcaa gcgtgcatga caccggcgac cttcgaccgg tagggcacat
      601 cggcggcctc ttggatgggc aacgcatcca tgtcggcccg gagcaggacc gtgggcgcga
      661 gccgcgggcc acgaatgagg cctaccacgc cggttccgcc gacgcccgcg cggacgtgga
      721 tcccgagtga ccgcaggtgc cccgatacca gcgccgccgt ttgggtctcg ccataggcca
      781 gctccgggtg ctgatggatc tggcgtcgcc actgcacgag ctgcctcttc atccgaaggg
      841 catgggcgag gaaggggtct tgggcgggct tgcggggcat ggacgatcct cctttggggc
      901 catcacctac cacggactct gtgacacgtt gaacagggtg gtgtcgaaca cattcacatc
      961 gggtggggcg gtcacggcgt ggagtacggc tcgggccacg gtctcgggct tcagccagtc
     1021 gggcttggga gtgcccgagg gattggtcag cgaggtgtcg accacgccgg ggttgaccat
     1081 gccgacggcg atgcgaaccc ccgttccgtg gagctggcgc gcgacggact gggtcatggc
     1141 ccggaggccc tccttgctgg cgttgtaggc acccgcatca ccggcctcgg tccagtgcga
     1201 cctgaccgaa ccaatgttga tgatcttccc cacggcgccg ttgtcgcgca tgaggcggat
     1261 cgcgcgctgc atgcagccat aggcggcgac cacgttcagc tccaggcacc gtcgcaggtc
     1321 ggaggctggc agttcctcga ccgggccaaa gggtgcgatg cctgcattgt tgaccaggat
     1381 gtccagccgg ctgaagatcc ggtcgatctc ggcgaacaga cgatcctgga atgcctcgtc
     1441 agtgatatcg ccctcgatgg ccagggcgca tccgccgccg gatcggatcg catcgacgac
     1501 ggtggccagc tccatcgggg tgcgagatgt gaccaccacg gtggccccag cctccgccag
     1561 ggcactggcc acggccgcgc cgatcccgcg tcccgcgccg gtgacgagcg cgatcttgcc
     1621 ctcgagcggt ctcacatcga tgcctcctgg ccgtggccat ccggcggctt catccgcgca
     1681 gagccagggg ttcgcagccg agacggaggg atcctgcagc ggcgacggcg gtagtcggcc
     1741 ctccccatgc acacgggctc gcgaggccgg cgccgacgcc ctggttcgta ttgggtcgat
     1801 tggggtatgt acaccccgga agccggtaga agcgtcccga cgcgaagcga agctcgggag
     1861 ggcccgggcg ccggaggttg tcttggcatc aactcctatc gatctgctcc cgaacgtgaa
     1921 tgccgtctgc gaggagctcc gcaggcgtat ggcggggatg tcggccgaac agaaggcggc
     1981 attcctgttg gctagtgcgc gcctggcggc agctcaagcc gatagcctgt cctctgacgg
     2041 acgccacagg accgaagtcg tcgtcgacca gtgcggctcg tgctaggatt cggctgcggc
     2101 gagcgcctct ttgatcagtg tctcgagatc ggccaagcgg aatggtttgc ggatgaacca
     2161 tgcccgccca agcccgtcca gatgccgggt cgcatcttgc tcggagtaac cgctcgcgac
     2221 aatgatcggc acatcggggc gccgctggcg gatagcgtcc agcgcctcct cgccgctcat
     2281 gcgcggcatg cgcatgtcga ggagcaccaa gcg
//
LOCUS       DAOSPL010000765         2310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46442_length_2310_cov_4.157428, whole genome shotgun sequence.
ACCESSION   DAOSPL010000765 DAOSPL010000000
VERSION     DAOSPL010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46442_length_2310_cov_4.157428"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="PLQ54_13435"
     CDS             <1..964
                     /locus_tag="PLQ54_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOS94316.1"
                     /translation="TTLSAFGLDVAARDLARHQAFFAGLSPAEHIERVFELARVSHVV
                     MTNDPFDAAERAVWLEGVERDPRFLPALRVDRLMGAPESVAAALAAAGHAVTSLGALC
                     DTARRFLDEWADRMDPIYLAMSLPSDYTFPEASLRGRMMAECIVPFCRERGLPLALMI
                     GVKRQVNPRLGLAGDAVGRCSVDAVEALCAQSPDVRFLVTLLSRENQHELCVSARKFP
                     NLMPFGCWWFLNNPSIIEEMTLERLELLGLSFIPQHSDARVLDQLVYKWSHSRAVIAD
                     VLCRKYDSVLRTGWALTEDEIRRDAEMLLRGNFIQFARIESRVG"
     gene            1003..2295
                     /locus_tag="PLQ54_13440"
     CDS             1003..2295
                     /locus_tag="PLQ54_13440"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOS94317.1"
                     /translation="MASKLAALGGDPILAPGSVRPWPHITDEDRRAVAETVAVEDLGQ
                     KRQENEEGLAADFAAYLGVKHVVPVNSGTAALHCAVGGCGILPGDEVICPAFTYWATA
                     AAVLHHNAIPVFVDIEPETWTMDPARLEGAITERTRAIMPVHIHGMPADMDPIVDVAK
                     RHGLKVIEDCAQSHGARYKGRLTGTIGDAAGFSLQASKLLTTGSYGGLFATDDDAIAH
                     HARLMQYLGEVVTPGRERAEQKYIHYGMGWMYRGDVFGQAFARSQLKRLDSNNDLRRR
                     NADLLSELLAPIPGVTTPVVPEGRGMAYYSYTVSFRPDQLGLDVSPEEFRGRAERALG
                     AEGVPVGRWQAIPVPSQDIFQTKGGYGRGCPWDCKHYSGNVSYDPAQFPVTLEFLRSH
                     TYVHAVWPPNGPELMRQIANAFEKVLRQPEALMQIEMG"
BASE COUNT          379 a          757 c          782 g          392 t
ORIGIN      
        1 taccacgctg tcggcatttg gcctcgatgt cgccgcgcgg gacttggcac gtcatcaggc
       61 gttcttcgca ggcctgagtc ctgcagagca tatcgagcgt gttttcgagc tggctcgggt
      121 ctcccacgtg gtgatgacga atgacccgtt cgatgcggcc gagcgggccg tctggctcga
      181 gggggttgag cgcgatccac ggttcctgcc cgcgctccgc gtcgaccggc tcatgggcgc
      241 cccggagtcg gtggccgcag ctttggccgc cgccgggcac gccgtgacct cgctcggcgc
      301 cctgtgcgat accgcgagac ggttcctcga tgagtgggcc gaccgcatgg acccgatcta
      361 cctggccatg tccctgccct cggattacac cttccccgag gcctcgctgc gcgggcgcat
      421 gatggccgag tgcatcgtgc ccttctgccg ggagcgagga ctcccgctcg cccttatgat
      481 cggcgtgaag cggcaggtga atccgcgcct ggggctggcc ggcgacgccg tcgggcgatg
      541 ctcggtcgac gccgtcgagg ccctgtgcgc ccagagtcct gacgtgcgct tcctggtgac
      601 gctactctcg cgggaaaacc agcacgagct gtgcgtctcg gctcgcaagt tcccgaacct
      661 gatgcctttc gggtgctggt ggtttctgaa caacccgagc atcatcgagg agatgacgct
      721 cgagcggctg gaactgctgg gcttgagctt catcccgcag cactccgacg cccgcgtgct
      781 ggatcagctc gtctacaagt ggtcgcattc gcgagccgtt attgcggacg tgctgtgccg
      841 caagtacgat tcggtcctcc gcactggctg ggcgctcacc gaggacgaga tccgcaggga
      901 tgcggagatg ctgctacgcg gcaacttcat tcagttcgcc agaatcgaga gtcgagtcgg
      961 ctgacagccg catcgaaagg cacgagaggg aggcacgcac gtatggcttc gaagctggca
     1021 gcgctgggcg gggatcccat cctggcaccc ggttcggtgc gcccatggcc gcatatcact
     1081 gatgaggacc gccgggcggt tgcggagacc gtcgcggtcg aggatctggg ccagaagcgg
     1141 caagagaacg aggaaggcct ggcggccgac ttcgccgcgt acctcggcgt caagcacgtc
     1201 gtgcccgtca acagcggcac cgccgcccta cactgtgcgg tgggagggtg cgggatactc
     1261 ccgggagacg aggtcatctg tccggcgttc acatactggg ccaccgctgc agccgtgctc
     1321 caccataacg ccattcccgt gttcgtggac atcgagcccg agacctggac catggacccg
     1381 gcgcggctcg aaggtgccat caccgagcgc actcgagcca tcatgcccgt gcacatccac
     1441 ggtatgccgg cggacatgga tcccatcgtc gacgtcgcca agcgccatgg cctcaaggtc
     1501 atcgaggact gcgcgcagtc gcacggcgcc cggtacaagg ggcggctgac gggtactatc
     1561 ggcgatgccg ctggcttctc gctacaggcg agcaagctac tgacgaccgg gagctacggt
     1621 ggcttgttcg ccaccgacga cgacgccatc gcgcaccacg cgcggctgat gcagtatctg
     1681 ggcgaagtcg tcacacccgg ccgggagcgg gcagagcaga agtacatcca ctatggcatg
     1741 ggctggatgt accgcggcga cgtgttcggc caggccttcg cgcgcagcca gctcaagcgc
     1801 ttggactcga acaacgatct ccgccgtcgc aacgcggacc tgctgagcga gctgctggct
     1861 cccatccctg gcgtcaccac ccccgtggtg cccgagggga gggggatggc ctactacagc
     1921 tacacggtga gcttccggcc cgaccagttg ggcctggacg tgtcacccga ggagttccgg
     1981 ggccgggccg agcgcgcgct tggcgccgag ggcgtgcccg tcggccgatg gcaggccatc
     2041 cccgtgccct cgcaggatat cttccaaacc aagggcggct acggtcgcgg atgcccgtgg
     2101 gactgcaagc actactcggg caacgtcagc tacgatccgg ctcagttccc ggtcaccctc
     2161 gagttcctgc ggtcgcacac ctacgtgcat gccgtgtggc cgccgaatgg cccagagctg
     2221 atgcggcaga tcgcgaacgc cttcgagaag gtgctgagac agcccgaggc gctgatgcag
     2281 atcgagatgg ggtaggggac ggcacggagt
//
LOCUS       DAOSPL010000766         2310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46444_length_2310_cov_3.823060, whole genome shotgun sequence.
ACCESSION   DAOSPL010000766 DAOSPL010000000
VERSION     DAOSPL010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46444_length_2310_cov_3.823060"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..2100)
                     /locus_tag="PLQ54_13445"
     CDS             complement(97..2100)
                     /locus_tag="PLQ54_13445"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOS94318.1"
                     /translation="MSGFSSPRTALTRRGMAIALAGMVCGLTMAVAVATDDQTPQVKI
                     VSPKPGETVSGNRIIVTVEYFSRSGAPIQFVELWVDGERQARDKLGTPRTSGRVQYAW
                     NGSAFTPGMHGLEALAEDTKGYQGTAVVSVTLAAAGQTLEGISPFITVRGPRNGERVS
                     GKITIAVDVQPGKAEFVYLMQRDPAGKQDRVLAATTQAPYEFHLSNLAEGTQQVYAVA
                     KTGVGEIQSELLSFEVVSRGGGTYTPQELPAPNLTPTAVASGPELALPELPLPPREAQ
                     EIGGGLVGRTLVLASAGAVRVISTPRPMVLAAKPEPQPVAVIAPTTPVAQPSAPVAPA
                     LPEPSMAARAVPGPALAPVSATVVSAPKVVARPEAAKPQATPPAAAAPVVVGPVAPMG
                     AVAAVAKVDLPKVTPAPAPVEPPSQVPPAPAATTEGTPGYVRSAAPTPGPMRIATVPE
                     PSPVATLASSRPLAPSVDAETPSAAPVARVAHAAQASGVHKPMIRIHVVRAGENLQAV
                     SRRYGVSASRIAQANNIPIEGALDSGSRLLIPPQTTILFDDVPIAFDVEPQIINGIAV
                     APIRHMLEAAGGTVYWIGDKGLVRAEVGDRNIEVRVGSREARVNEEVVLLDLAATMKR
                     DRVIVPVRFVGKALDVSVRYDPATENVHIVSHKPLDASSSPAA"
BASE COUNT          344 a          794 c          808 g          364 t
ORIGIN      
        1 cgcgagagga ccgccgtgcg cgggctcctt cacgcctcgg atccgatgtg agtccctgtt
       61 tccacacctg atccggggct ctggcccacc accccgctag gctgctgggg aggaagaagc
      121 atccagcggc ttgtgactca cgatgtgcac gttctcggta gccggatcgt agcggaccga
      181 gacatcgagc gccttgccga cgaagcgcac gggcacgatc acgcgatctc gtttcatggt
      241 ggcggccaga tcgaggagga cgacctcctc gttcacacgc gcctcccggc tcccgacccg
      301 cacctcgatg ttccggtctc cgacctctgc gcgaacgagc cccttgtcgc caatccaata
      361 gacggttccc cccgcggcct cgagcatgtg ccggatgggg gctaccgcga tcccgttgat
      421 gatctggggt tcgacatcga aggcgatggg cacgtcatcg aacagtatcg tcgtctgcgg
      481 cggaatcagc aagcgagagc cgctgtccag cgccccctca atcgggatgt tgttggcttg
      541 cgcaatgcgg ctggccgaga ccccgtaccg gcgcgagacg gcctgcaggt tctctccggc
      601 ccgcaccacg tgaatgcgga tcatcggctt gtggacgcca ctggcctgtg ccgcatgggc
      661 cacccgcgcc accggcgcag cggagggcgt ttccgcgtcc accgagggcg ccagggggcg
      721 cgacgaggcg agggtagcca ccggggatgg ctcgggcacc gtggcgatgc gcattgggcc
      781 aggcgtcggc gccgccgagc gcacataccc gggggtgccc tctgtcgtgg ctgcgggcgc
      841 cggcgggacc tgactgggtg gctcgaccgg cgccggggcc ggtgtcacct tcgggaggtc
      901 caccttggcc acggccgcca cagcgcccat gggggcgacg gggccgacca caaccggtgc
      961 cgccgctgcg ggcggagtgg cctgtggttt cgcggcttcg ggccgcgcca ccaccttggg
     1021 ggccgacacg accgtggccg agaccggcgc cagcgcgggg ccgggcacgg ctctggccgc
     1081 catggacggc tcggggagcg cgggcgccac cggggccgac ggctgagcca ccggcgtcgt
     1141 tggagcgatc acggccacgg gctgcggctc aggtttggcc gccaggacca tcggcctggg
     1201 tgtcgagatc acccggacgg caccggcact ggccaggacc agcgtgcggc ccaccaggcc
     1261 cccgcctatc tcctgggctt cacgcggtgg cagagggagc tccggcagtg ccagctcggg
     1321 accggaagcc accgcggtgg gcgtgaggtt cggggccggg agctcctgtg gcgtgtaggt
     1381 gccaccaccg cggctgacga cctcgaagct cagcaactcg gactggatct cgccgacgcc
     1441 cgtcttggcc acggcataga cctgctgtgt gccctcggcc aggttcgaca ggtggaactc
     1501 gtaaggggcc tgtgtggtgg cggcgaggac gcggtcctgt ttgcccgcgg ggtcgcgctg
     1561 catgagatag acgaactcgg ccttccccgg ctgcacatcg acggcgatgg tgatcttgcc
     1621 cgagactcgc tctccgttcc gggggccgcg cacagtgatg aagggcgaga tcccctccag
     1681 cgtctggccc gcggccgcga gcgtcacgga gaccacggca gtgccctggt aacctttggt
     1741 gtcctcggcg agagcctcga ggccgtgcat gcccggagtg aaggccgaac cgttccaggc
     1801 atactggacc cgaccgctgg tgcggggcgt ccccagcttg tcgcgggcct ggcgttcacc
     1861 gtcgacccac agctccacga actggatcgg cgcgccactg cggctgaagt actccaccgt
     1921 cacgataatg cggttcccgc tgacggtctc gccaggcttg ggcgagacga tcttcacctg
     1981 aggggtctgg tcgtctgtgg cgaccgcaac ggccatcgta aggccacaca ccatgccggc
     2041 cagagcaatg gccatccccc tccgggtgag cgcagtcctt gggctcgaga atccgctcat
     2101 gcggccgtac ctcctcgcca cggcaccgag tgccgcaggc gaaactgcgc gcagaacgcc
     2161 ctggggcgct gtgcgccggt ctccggagag tatgctccgg cacagtttcc accgccagca
     2221 ggctcgacac cttcctgccg gcagcaaaat ggctcattca ggtggatata cgcagggagc
     2281 tgtgccgatc cctgccttcg gttgacggtg
//
LOCUS       DAOSPL010000767         2310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46451_length_2310_cov_3.050998, whole genome shotgun sequence.
ACCESSION   DAOSPL010000767 DAOSPL010000000
VERSION     DAOSPL010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46451_length_2310_cov_3.050998"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1746)
                     /locus_tag="PLQ54_13450"
     CDS             complement(<1..1746)
                     /locus_tag="PLQ54_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOS94319.1"
                     /translation="MVGESYCPQCQTRLPASATAPIPWRYCPVCGAQIAYDCPHRCGK
                     LLPVRLRYRCADDIGEGCGLRLRACLNPSCARYFAPSAPTDAAELARALACVQCGSPL
                     GDFDQTFDWLQMGVSRDAYLSDGAARRRLWTSAPGPPELPVDGSGVTPRAELGACGAP
                     ALRLGHLFAGSESGRIVRWDVTSVGVDRSGLGDPWLPGDPDDFAGASSRLSLRAPMHT
                     SELYLHMRDAGGRRLFVIDPATGARLLRWDVPGFDDYSATVLGRWLAVLGLRREGDSV
                     TEHLAVFDLPALMAEVGSSAARTVSASLRCAKAVRAWTATDRLHEFYRSRVECWPRRV
                     CRVGDCLYALSTANQLWRLRTDDSTADWEDLGGFGPPPEHTSTTQLAHSMHKGVLVTA
                     WTVQQRLQVAVHRCDESRTGIVTLRTGLPPEAAQLAMALDDERLYLCDTAGVDLLWWT
                     PLEDLRDPRFRGPTCGEVPLLEGAQYRVDSLYRADTLDESGRWRPHVVVHVRRGGAAQ
                     AEGTIEAAAGLVLVDALFGGRRGRMPTALEDAGPCLISRAGDTEVGRSHWLPAESDPR
                     WLLLRDRRLLYCGSVE"
     gene            complement(1756..>2310)
                     /locus_tag="PLQ54_13455"
     CDS             complement(1756..>2310)
                     /locus_tag="PLQ54_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94320.1"
                     /translation="AELASDAVNAMRDEGLLLAADWVQAHGLSPLSHFMAVLGRSAAE
                     LSSLAAEPEAPAAAALAGLAAWLADGADDALASPGRAAPHVPDRYGGFRLLIEGLGSL
                     RSRCVAYVREREGFAAIGRQGERLDPSRHEVAGGRPTPSVSLHDTVAEVVRPGLMKGE
                     QIVFPARVVVFCDPSIPNPYTWAG"
BASE COUNT          402 a          819 c          800 g          289 t
ORIGIN      
        1 ctcaacggac ccgcagtaca ggagccggcg atcgcgcagc aggagccatc gaggatcaga
       61 ttccgccggc agccagtggc tcctgccgac ctcggtgtcg cccgcgcgcg aaatgaggca
      121 cgggcccgcg tcctcgagcg cagtcggcat ccgcccacgc cggccgccga aaagcgcatc
      181 aacgagcacc agcccggcgg cggcttcgat ggtgccctcg gcctgcgccg ccccgccgcg
      241 gcgcacgtgc acgaccacat ggggccgcca gcgccccgac tcgtcgagcg tgtcagcccg
      301 gtagagggag tcgacgcggt actgagcgcc ctcgagcaat ggcacttcgc cgcatgtcgg
      361 tccgcgaaag cgaggatccc tgaggtcctc cagcggcgtc caccagagca gatcgacgcc
      421 cgccgtgtcg cacaggtaga gccgctcgtc atccagagcc atcgcgagtt gcgccgcctc
      481 gggcgggagc ccagtccgca aagtgacgat gcccgtcctg gactcgtcgc accgatggac
      541 cgcgacctgg agtctctgtt ggacggtcca ggcggtgacc aggacaccct tgtgcatcga
      601 gtgggccagt tgcgtggttg aggtatgctc gggtggggga ccgaagccgc ccaggtcctc
      661 ccagtcggct gtggagtcgt cagttcgcag gcgccagagc tggttcgccg tcgagagggc
      721 atagaggcag tccccgacgc ggcatacccg gcgcggccag cattcgacgc gcgagcggta
      781 gaactcgtgg aggcggtctg tggccgtcca ggcacgcact gccttggcgc accgaaggga
      841 cgccgaaacc gtccgcgcag cgctcgatcc cacctcggcc atgagtgccg gcaggtcgaa
      901 cacggccagg tgctccgtga ccgagtctcc ctcacgccgg agccccagaa ccgcgagcca
      961 gcgcccgaga actgtggcgc tgtagtcgtc gaaccctggc acatcccacc ggagcaggcg
     1021 cgctccggtg gcggggtcga tgacgaacag ccgccggccc cccgcgtccc gcatgtgcag
     1081 gtagagctcg gacgtgtgca tgggagcccg gagactgagg cgggaggacg ccccggcaaa
     1141 gtcgtctgga tcacccggaa gccacgggtc gcccagtccg gaccgatcga cgccgaccga
     1201 ggtgacgtcc catcgcacta tccggccgct ctcggagccg gcaaacagat gccccaacct
     1261 gagggcaggc gccccgcagg cacccaactc agcgcggggc gtgaccccgg agccatcgac
     1321 gggcagttcg ggcggccccg gcgccgacgt ccacagacgg cgccgggccg cgccatcgct
     1381 cagataggcg tcacgcgata cgcccatctg gagccaatcg aacgtctgat cgaagtcgcc
     1441 caatggcgat ccgcactgca cgcaggccag cgcccgggcg agctccgcgg catccgtggg
     1501 agccgaaggg gcgaagtagc gtgcgcagct cgggttcagg caggcccgga gacgcagtcc
     1561 acagccctcg ccgatgtcgt cagcgcaacg gtaccgcaga cggacaggca acagcttccc
     1621 acaccggtga gggcagtcgt acgcgatctg tgccccacag accgggcagt agcgccacgg
     1681 gatcggtgcc gtggccgagg ccgggagccg cgtctgacac tgggggcaat aggactcgcc
     1741 aaccatccgc gcctctcatc ctgcccacgt gtacgggttg ggaatgctcg ggtcgcagaa
     1801 gaccaccact cgggccggga agacgatctg ctcgcccttc atgagtccgg gacgaacgac
     1861 ttcggccacg gtatcgtgca gagacacgct gggtgtggga cggccgccgg cgacttcatg
     1921 tcgcgacgga tccaggcgtt cgccttggcg gccgatggcg gcgaatccct cacgctcgcg
     1981 aacgtacgcg acgcagcggg accgcaggga gccgaggccc tcgatcagaa gccggaagcc
     2041 accatagcgg tcggggacat ggggggcggc acgcccgggc gaagccagcg cgtcgtcggc
     2101 accatcggca agccacgcgg ccagtcctgc caacgcagcc gccgcggggg cctccggctc
     2161 ggcggccaga ctcgacagct ccgcggccga acggcccagg accgccatga agtgagagag
     2221 cggcgacagg ccgtgggcct gcacccagtc ggctgccaga aggaggccct cgtccctcat
     2281 cgcgttcacg gcatcgctgg ccagctcggc
//
LOCUS       DAOSPL010000768         2308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46522_length_2308_cov_2.565024, whole genome shotgun sequence.
ACCESSION   DAOSPL010000768 DAOSPL010000000
VERSION     DAOSPL010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46522_length_2308_cov_2.565024"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PLQ54_13460"
     CDS             <1..619
                     /locus_tag="PLQ54_13460"
                     /inference="COORDINATES: protein motif:HMM:NF028184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH36 C-terminal domain-containing protein"
                     /protein_id="HOS94321.1"
                     /translation="IDTCASGGRRNDLESLRRSVPLLRSDYILEPVGQQLHTYGLAMW
                     VPFYGTGVNTGDPYTFRSALCAHMTFCYDMRNREYDFDPVRERVAEWERLAPYLLADY
                     YPLTAYQPGGDGWIAWQFNEPETGGGMVQAFRRAESIYESARLRLHGLEAGAQYEFRD
                     IDTGVARLLTGAEVMNQGISIPIDQAPGAAIVEYHIVGAGGQTRG"
     gene            complement(616..1428)
                     /locus_tag="PLQ54_13465"
     CDS             complement(616..1428)
                     /locus_tag="PLQ54_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94322.1"
                     /translation="MAIDYQIDRNCKLKRHLGRQGLVDHLKRQMILDLMSLAGRRSLV
                     RPVGRRGVEPPPTISEVARERLASRLRVECEALSSYSRDCAVCPANEFAERRWGIGGC
                     VGFVTLPIERDLERACAAYIVGTATHGDVTRLAWWDGIAAAATRELTLYRFLRAREDA
                     GGPVFFQRRRALPIRLAAETMGATLTTDAVFEFLLGFVDGDPPDAAAVGEFCAGLRRD
                     LEASLGGDGLVRRLERCRSIDDILRYGQACAKAAALGLPMWVGGPFAPPPSL"
     gene            1606..2301
                     /locus_tag="PLQ54_13470"
     CDS             1606..2301
                     /locus_tag="PLQ54_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOS94323.1"
                     /translation="MGSIAERLAAVLDRIGRACERSGRAPHSVTLVAVTKQRSVAEIQ
                     EVLSAGAVNIGENYVQEALAKHAELGRGPVWHFIGHLQRNKARDAVRLSDWIHSVDSL
                     RLADELSRRAVEQGKRQRVLVEVNVSGEATKSGVAPDQLAALLEGMRSLEGLTVEGLM
                     TMAPWVDDAEQVRPVFAETRRLAERCAAAGLLPQGAHLSMGMSSDFEVAVEEGATLVR
                     VGTAIFGPREAAS"
BASE COUNT          401 a          778 c          766 g          363 t
ORIGIN      
        1 gatcgacacc tgtgcctccg gcggtcgacg gaacgatctc gagtcgcttc gcagatccgt
       61 accgctgttg cgcagcgact acattctcga acccgtcggg cagcagctcc atacctacgg
      121 cttggccatg tgggtgccgt tctacggcac gggcgtcaac accggagatc cctacacatt
      181 ccggagcgcg ttgtgtgcgc atatgacatt ctgctatgac atgcgcaacc gggagtacga
      241 cttcgatccg gtccgcgaac gggtggctga gtgggagcgg ctagccccct atctcctggc
      301 cgactactac cctctgaccg cctaccagcc cgggggcgat ggttggatcg cttggcagtt
      361 caacgagccc gagacgggtg gcgggatggt ccaggcgttc cggcgcgccg agagcatcta
      421 cgagtcggcc cggctccggc tgcatgggct cgaggccggc gcgcagtacg agttccgcga
      481 catcgacacg ggtgtcgcta gactgctcac tggtgctgaa gtcatgaatc agggcatctc
      541 gatacccatc gatcaggctc ccggagccgc catcgtggag taccacatcg tcggtgccgg
      601 cgggcagacg cgaggctaga ggctcggcgg cggcgcgaag ggtcctccga cccacatcgg
      661 cagaccgagt gcagcggcct ttgcacaagc ttggccgtac cggagaatgt cgtcgatcga
      721 ccggcatcgc tcgagccgcc ggacgagccc gtctccaccg agagaggcct caaggtcgcg
      781 acgaagccct gcgcagaact cacccacggc cgcggcatcg ggcgggtcac cgtccacgaa
      841 gcccaatagg aactcgaaca cggcatcagt ggtcagtgtc gcgcccatgg tctcggcagc
      901 gagccggatg ggaagcgccc ggcggcgctg gaagaacacg ggcccaccgg cgtcttcgcg
      961 cgctcgcaaa aaccggtaca gggtcagctc ccgcgtggcg gcggcggcga tgccatccca
     1021 ccacgccagg cgagtgacat ccccgtgcgt cgccgtcccc acaatgtacg ccgcgcacgc
     1081 gcgttcgagg tcccgttcga taggcagcgt gacgaacccc acacagccgc cgatgcccca
     1141 tcggcgctcc gcgaactcgt tagcgggaca gaccgcgcag tctctcgagt aggagctcaa
     1201 cgcctcgcac tcaacccgca gtcgcgacgc cagtcgctct cgagccacct cgctgatggt
     1261 gggcgggggc tccacgcccc gccgccccac gggtcgcacc aacgaccggc gtcccgcgag
     1321 actcatcagg tcgaggatca tctgcctctt gaggtggtcg accaggccct gacgccccag
     1381 gtggcgcttg agtttgcagt tccggtcaat ctggtagtcg atggccaccg gcgccgccgc
     1441 ccccttatca gggctcgcaa tactgcacta tcccggggca caaaccactc cagcaacact
     1501 ctggttcgga tcagcggcct gaactgcctc gggcacccgt gggttatgcc cttgccggtt
     1561 cgcccggcgc atccggcact gttcggccaa aggagtggcc cgcacgtggg cagcattgcc
     1621 gagagactgg ccgctgtcct ggaccgcatc ggccgggcct gcgaacggtc gggacgtgcg
     1681 ccccatagcg tcactctcgt cgcggtaacc aaacagcgat ctgtggccga gatccaggag
     1741 gtactctccg ccggagccgt gaacatagga gagaactatg tgcaggaggc tctggcgaag
     1801 cacgctgagc tcggcagggg gccggtctgg cacttcatcg ggcaccttca gcggaacaag
     1861 gcccgggatg ccgtccggct aagcgactgg atccactccg tcgacagcct gcggcttgct
     1921 gacgagctct cgcggcgcgc cgttgagcag ggaaagcgcc agcgagtact ggtcgaggtc
     1981 aacgtctcgg gagaggcgac caagtccggc gtggctccag accagctcgc cgcgctgctc
     2041 gagggcatgc ggtcactcga aggcctgacc gtcgagggac tgatgaccat ggcgccctgg
     2101 gtcgacgacg ccgagcaggt caggccggtc ttcgccgaga cccgccgcct cgccgagcgg
     2161 tgtgcggccg ccgggctcct gccacaggga gcccatctgt cgatgggcat gagtagtgac
     2221 ttcgaggtgg cggtggagga gggcgcgact ctagtgcgag tagggactgc catcttcggg
     2281 ccgcgagagg ccgcgagctg acgccgtg
//
LOCUS       DAOSPL010000769         2306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46580_length_2306_cov_3.309196, whole genome shotgun sequence.
ACCESSION   DAOSPL010000769 DAOSPL010000000
VERSION     DAOSPL010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46580_length_2306_cov_3.309196"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PLQ54_13475"
     CDS             complement(<1..338)
                     /locus_tag="PLQ54_13475"
                     /inference="COORDINATES: protein motif:HMM:NF012299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOS94324.1"
                     /translation="MGWHGLSGRGSRVAVVGTGVTGLETARVLLSLGTEVTLYDTREE
                     SAIPEDALRRARSMGCTVRLGTHDGLGDHAVVVTSPGVPIESPYFGPARERGVEVISE
                     IEAAFRLWPG"
     gene            complement(380..1345)
                     /gene="mraY"
                     /locus_tag="PLQ54_13480"
     CDS             complement(380..1345)
                     /gene="mraY"
                     /locus_tag="PLQ54_13480"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOS94325.1"
                     /translation="MFWIAAAAVAFFVATSVAWALGPSTIAWARRRQPDARPREDVPQ
                     THLRKVGTPSMGGGFILPSACAGMAVVIWKPEVVGVVLSMALFGLLGLADDLRKIRSS
                     RGRGLKAREKLVIQALLGAGLALYAHRVLGRPDGLSLPSGSVAHLGWGYYVLGAFVAM
                     AVTNAVNLTDGLDGLAAGSLVPTLLAVMTVCLSVGPEACGVAAAAVAGACVGFLRFNL
                     SPARIWMGDTGSLGLGGALSALAIFGGIEWFLAVAGAVLAAEALSVIIQVVSFKTTGR
                     RVFRMSPLHHHFELVGHGETAIVKGFWAASLAASCLAVGLALLVH"
     gene            complement(1351..>2306)
                     /locus_tag="PLQ54_13485"
     CDS             complement(1351..>2306)
                     /locus_tag="PLQ54_13485"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOS94326.1"
                     /translation="GDVGSLSRGQVRADDATEYLVLEFAMRGPGQIAWLADAARPRVG
                     AITNIDAVHAELLGTVEAIAEAKAELLAALPRDGAAALPADDVYFGFLRPRALCPVMS
                     FGLSRGDVRARIASEDASSGRSTVAVTTPEGSADIKLRCIGPHYAYDAACAAAAGLCM
                     GLPLSAIAEGLEGYAPGRGRGEVCVSARGVRVFDDAYNASPRSVKAALYVLSVQPARR
                     VAVLGDMLEMGDAEETGHRAVGAYAAELSIDRLVAVGSRARWIADEAVSRGMSPEHVH
                     HFMNNDDAATFLLGVLAPGDTVLVKGSRGQRMESIVAALLD"
BASE COUNT          428 a          889 c          693 g          296 t
ORIGIN      
        1 atgccgggcc agaggcggaa agccgcctcg atctcgctga tcacctcgac gcctcgctcg
       61 cgcgccggcc caaagtacgg tgactcgatg ggaaccccag ggctcgtaac caccaccgcg
      121 tggtcgccca ggccgtcatg agtgcccagc cgcacggtgc agcccatgct gcgagctctc
      181 cgcagcgcgt cctccggaat cgccgactcc tcgcgtgtgt catagagggt cacttcggtg
      241 ccgaggctca ggagcacccg agcggtctcg agccccgtca cgccggtccc caccacggcg
      301 actcgcgatc ccctcccgct cagcccgtgc caacccatcg ccatccccag tcccgacaat
      361 cctctccgca gcgaaaccac taatggacca ggagcgccaa ccccacggcc agacacgagg
      421 cggccagtga agcggcccag aagcccttca caatcgccgt ctccccgtga ccaaccagct
      481 cgaaatgatg gtgaagtggc gacatgcgaa acactcgccg gccggtggtc ttgaacgaga
      541 cgacctggat gatcaccgac agagcctcgg ccgccaggac ggcgcctgcc accgcgagaa
      601 accactcgat cccaccaaag atcgccagcg ccgatagtgc cccacccagc cccagcgatc
      661 ccgtgtcccc catccagatc cgcgcaggac tcaggttgaa gcgcaggaac cccacacacg
      721 cccccgccac cgctgccgcg gccacaccgc aggcctcagg ccccaccgac aagcacaccg
      781 tcatcacggc gaggagtgtg ggcaccaagg agcccgccgc gaggccatcc agcccatccg
      841 tcaggttcac cgcgttggtg acggccatgg cgacaaatgc tccgaggacg taatagcccc
      901 agcccaagtg cgcgacggag ccgctgggca gagacagccc gtccgggcga ccgagcactc
      961 ggtgggcata gagcgccagc ccggcgccca gaagagcctg tatcacgagc ttctcgcgcg
     1021 ccttcaggcc ccggcctctg gacgagcgaa tcttgcgcag gtcatcggcg agcccgagca
     1081 ggccgaagag cgccatggac aacaccacgc caaccacttc gggcttccag atcaccacgg
     1141 ccatcccggc gcaggccgat ggaaggatga acccaccgcc catgctcggc gtgcccacct
     1201 tgcgcaggtg ggtctgcggc acatcctcac gggggcgcgc atcgggctgg cggcggcgag
     1261 cccaagcaat ggtcgaaggg cccagtgccc aggcaacgct cgtggccacg aagaaggcca
     1321 cggcggcggc ggcgatccaa aacatggcag tcagtccaat agggcggcga cgatgctctc
     1381 catccgttgg cctcgcgatc ccttgacaag cacggtgtct ccgggcgcga gtacccctag
     1441 aaggaacgtc gcggcgtcat cgttgttcat gaagtgatgg acatgctccg gactcatgcc
     1501 ccgtgacacg gcctcgtcgg ctatccagcg cgcccgtgaa cccacggcaa ctagccggtc
     1561 gatcgaaagc tcggccgcat aggcaccgac ggcccggtgc cccgtctcct ccgcgtcgcc
     1621 catctcgagc atgtcgccca acacggccac gcggcgcgcc ggttgtaccg agagcacgta
     1681 gagcgccgcc ttgacgctcc ggggcgacgc attgtaggcg tcatcgaaca cgcgtacccc
     1741 gcgggcactg acgcacacct cgccccgtcc ccggccggga gcatatccct cgaggccctc
     1801 cgcaatggcc gacaggggca accccatgca caggccggct gccgccgcgc acgccgcgtc
     1861 gtacgcgtag tgggggccga tacagcggag cttgatgtcc gcacttccct ccggagtcgt
     1921 caccgccacc gttgaccgcc ccgaactggc gtcctcagat gcgatccgcg cccggacgtc
     1981 gccccggctc aggccgaagc tcatgaccgg gcacaaggca cgcggccgga gaaagccgaa
     2041 atacacatcg tccgcgggca gggcggcggc tccatcgcgc ggcagcgccg ccagcaactc
     2101 tgccttggcc tcggctatgg cctccaccgt ccccagcagc tccgcgtgca cggcatcgat
     2161 gttcgtgatc gcgcccaccc gtgggcgcgc cgcgtcagcc agccacgcga tctgccccgg
     2221 gccccgcatg gcgaactcca gcaccagata ctccgtcgcg tcatcggcgc ggacctgccc
     2281 gcgcgacagg ctcccgacat ccccgg
//
LOCUS       DAOSPL010000770         2306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46591_length_2306_cov_2.406486, whole genome shotgun sequence.
ACCESSION   DAOSPL010000770 DAOSPL010000000
VERSION     DAOSPL010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46591_length_2306_cov_2.406486"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2306)
                     /locus_tag="PLQ54_13490"
     CDS             complement(<1..>2306)
                     /locus_tag="PLQ54_13490"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOS94327.1"
                     /translation="SMRGGTVWFDDASLTLVEPAPPEPGTPAPTDGLPLDAIIIPDVS
                     GPHHGYLALEAQRILGTMVGAAPPIVSASAAPVGAEARHLWIGVVPPGRSYAAQLAEV
                     GDEGIVLDIGPEAVVCLGNTPRGVYYAVHELFHMLGCRWCWPGPLGEVVPKAERLDLP
                     PKLIVHAPDFTLRGGTMIQVYHDPVTGEAKHIDTESWVDWAARNRINRLKAGYATTWD
                     YGAIRGGGQDEIAGHSLYAILDPAKYFPDHPEYYPLVRGERTAVHSSGRPAQVCTSNA
                     EVIARVADYACDYFASHPLAKRFAVCAEDEPSYWCECDACKALDTQPVDWSRNGVDVM
                     PLTDRWLHFVNAIAERVEQQYPGKWVVTFAYASSRELPTRERPRPNVMIELTWWDECF
                     RHRIEDPNCAINVQGMERFHGWSRLAPLSLYRYLDYAHCESPGPYFHAEADLLRTAHA
                     GGVRNLADEWDTTFAASALLLNLRARLEWDLSTDVDAFITDWCQRVYGPAGDAMERYY
                     RRLERGVEESPGEHVTFNDLTKFTPEVLRDCHALLDEATAAAAGSDDIAGRIDRQRYS
                     LLYAEFDQVSEAATNDLALLQRKEALRARLWELVQRRQIAPVLDYWHRLTGEYQPPVA
                     ALTGRRVVELPETWQFRTDPDNTGEAQGWATAVPDAAWRPISIHKAWEEQGVEGYDGY
                     AWYTVEANIPAIPEPRVWLLCEAVDETFTLWINGEYIGVSEGDPVIVDAETGEAYLRP
                     RPDVVTSVTSRIEVRNQRKAQFAKLRDT"
BASE COUNT          353 a          791 c          789 g          373 t
ORIGIN      
        1 cgtatcgcgc agcttggcga actgcgcctt gcgctggttg cgcacctcga tgcgcgaggt
       61 gacggacgtg accacgtcgg gccggggccg cagataggcc tcgcccgtct cggcgtcgac
      121 aatgaccggg tcgccctcgc tgacgccaat gtactcgccg ttgatccaga gcgtgaacgt
      181 ctcgtccacc gcctcgcaca gcagccagac gcgcggctcg gggatggccg ggatgttcgc
      241 ctcgaccgtg taccacgcgt agccgtcgta gccctcgacg ccctgctcct cccaggcctt
      301 gtggatggag atgggccgcc aagcggcatc ggggacggcc gtcgcccagc cctgggcctc
      361 gcccgtgttg tccgggtccg tgcggaactg ccatgtctcc ggcagctcga cgaccctccg
      421 gcccgtcagc gccgccacgg gcggctggta ctcgcccgtc agccggtgcc agtagtcgag
      481 caccggcgcg atctggcgcc gctggaccag ttcccacagc ctcgcgcgga gcgcctcctt
      541 gcgctgcagc agcgcaaggt cattggtcgc ggcctcactc acctggtcga actcggcata
      601 gaggagcgag tagcgctgcc ggtcgatccg gccagcgatg tcatccgagc cggctgccgc
      661 agccgtggcc tcgtcgagta aggcgtggca atcgcggagc acctcgggcg tgaacttggt
      721 gaggtcattg aatgtcacat gctcgcccgg cgattcctcg actcctcgct ccagccgccg
      781 gtagtaacgc tccatggcgt cgccggccgg cccgtagacc cgctggcacc aatcggtgat
      841 gaacgcatca acatcggtgc tcaggtccca ctcgagtcgg gcgcggaggt tgagaagaag
      901 cgcagaagcc gcgaaggtcg tgtcccactc atcggccagg ttgcggactc cgcccgcgtg
      961 ggccgtccgc aggagatccg cctcggcgtg gaagtagggc ccggggctct cgcaatgggc
     1021 gtagtccagg taccggtaga gcgagagtgg cgccagccga ctccacccgt ggaagcgctc
     1081 catcccctgg acgttgatgg cgcagttcgg gtcctcgatg cggtgcctga agcactcatc
     1141 ccaccaagtg agctcgatca tcacgttcgg gcgggggcgc tcgcgggtcg gcagctcgcg
     1201 gctcgacgca taggcgaagg tcaccaccca cttacccggg tactgctgct cgacccgctc
     1261 cgcgatggcg ttcacgaagt gcagccaccg gtcggtcagt ggcatcacgt cgacgccgtt
     1321 ccggctccag tcgaccggct gggtgtcgag ggccttgcag gcgtcgcact cgcaccagta
     1381 gctgggctca tcctcggcac agaccgcgaa ccgtttcgcc aatgggtggc tggcgaagta
     1441 gtcgcacgcg tagtcggcca cccgggcgat gacctcggcg ttcgacgtgc agacctgcgc
     1501 cggccggccg ctggagtgca ccgccgtgcg ctccccgcgc accagcgggt agtactccgg
     1561 gtgatcgggg aaatacttcg ccggatcgag aatggcatag agcgagtgac cggcgatctc
     1621 atcctgcccg cccccgcgga tggccccgta atcccacgtg gtagcgtagc cggctttcag
     1681 acggttgatg cggttccgtg cggcccagtc gacccagctc tcggtgtcga tgtgcttggc
     1741 ctcaccggtc accgggtcgt ggtagacctg gatcatcgtg ccgccccgga gcgtgaagtc
     1801 gggcgcgtgg acgatgagct tcggcggcag atcgaggcgc tcggccttcg ggaccacctc
     1861 tcccagcggg ccgggccagc accagcggca tccgagcatg tggaacagct cgtgcaccgc
     1921 gtagtagaca ccccggggcg tattcccgag gcagacgacg gcttcggggc cgatgtcgag
     1981 cacgatgccc tcatctccca cttcggccag ttgggccgca tagctgcgac cgggcgggac
     2041 cacgccaatc cacaggtgtc tcgcctcggc ccccacggga gccgccgacg cggagacaat
     2101 gggcggggca gcgcccacca tggtgccgag gatccgctgg gcctcgaggg ccaggtagcc
     2161 gtgatgtggc ccggagacat cgggaatgat gatcgcgtca agcgggaggc cgtccgtcgg
     2221 cgcgggcgtg cccggttctg ggggagccgg ctcgaccagt gtgaggctgg catcgtcgaa
     2281 ccacacggtg ccgccccgca tcgact
//
LOCUS       DAOSPL010000771         2302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46692_length_2302_cov_3.507343, whole genome shotgun sequence.
ACCESSION   DAOSPL010000771 DAOSPL010000000
VERSION     DAOSPL010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46692_length_2302_cov_3.507343"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PLQ54_13495"
     CDS             <1..340
                     /locus_tag="PLQ54_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOS94328.1"
                     /translation="SGDEYTFMDLENYDQMELGRDIVGDQAKYLSDGLECQIVLFKGK
                     VITLEIPLFLELEVVQTDPGIRGDTATGGTKPATLAPGAVVMVPLFVNTGDVIKVDTR
                     TDSYIERVTK"
     gene            342..698
                     /locus_tag="PLQ54_13500"
     CDS             342..698
                     /locus_tag="PLQ54_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94329.1"
                     /translation="MTQSDKVSKTGARVSYLRGVLDGQKLSQDERLELVLSTFVDVSD
                     KLLEVLAEVREEQSEIRRLLASLQPGDPVDARPEFAPQLSCPQCGQRLAIGPEMLERG
                     PVELTCPECHLTIEVV"
     gene            714..1160
                     /locus_tag="PLQ54_13505"
     CDS             714..1160
                     /locus_tag="PLQ54_13505"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94330.1"
                     /translation="MDSAGIASSLAELVRVAEEEGLAGLRVARNGISVDFRRSAATAA
                     TTPLVPASTAEEPEPEQASTAPAAGGHELRSRLVGVFRRCQPGRDRPVVNPGDPVREG
                     QLLGYIESMRVMNDVTADVTGILDEFLVGDGEGVDYGRPIARFRVG"
     gene            1311..1646
                     /locus_tag="PLQ54_13510"
     CDS             1311..1646
                     /locus_tag="PLQ54_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94331.1"
                     /translation="MIGLLVSLIIILLVLLVGLGMMKTQGVGKSTPARVESKARGLQC
                     QTYLQQMRMMALINNEEKQEPTSLEDFRGAGELRCPVSGMRYYFSPARAGDPQTRGLY
                     CPYPEHWNF"
     gene            1701..>2302
                     /locus_tag="PLQ54_13515"
     CDS             1701..>2302
                     /locus_tag="PLQ54_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOS94332.1"
                     /translation="MFKRILVANRSEIAVRIIRACHELGIEAIAVYSEADREALHVQM
                     ADHSVCIGPPAARESYLNVPNIMSAAMIHGCDAIHPGLGFLAENADFAEICETCGITF
                     IGPTVEAIGKMGDKARARRIMHEAGVPVIRGTSEAVEDEASILKAAADFGYPLRVKAS
                     LGGGGRGIRIVHSEEELLRSVQLARAEAKAAFGSPDVYLER"
BASE COUNT          411 a          672 c          825 g          394 t
ORIGIN      
        1 gtcgggcgac gagtacacgt tcatggacct cgagaactat gaccagatgg agctgggccg
       61 ggacattgtc ggcgaccagg ccaagtatct atcggacggg ctggagtgcc agatcgttct
      121 gttcaaaggt aaggtcatta ccctggagat tccgctgttc ctggagcttg aggtcgtcca
      181 gacggacccc ggcatccggg gcgacaccgc cacgggtggg accaagccgg cgacgctggc
      241 gccaggggcc gtggtcatgg ttcctctgtt tgtgaacaca ggtgacgtga tcaaggtcga
      301 tacccgcacc gacagttata tagaacgagt gaccaaatag aatgacccag agcgacaagg
      361 tgtcgaagac cggtgctcgc gtctcgtacc tgcgaggggt cctggacgga cagaagctat
      421 cgcaggatga gcgcctggag ctcgtgctct cgacctttgt cgatgtgagt gacaagcttc
      481 tggaggtgct ggcagaggtt cgcgaggagc agtcagagat ccgtcggctt ctggcgagcc
      541 tgcagccggg cgaccccgtg gacgctcgtc ctgagtttgc gcctcagctg agctgccccc
      601 agtgcgggca acggctggcc atcggccccg agatgctcga gcggggcccc gtagaactca
      661 cgtgccctga gtgccatctc acgattgagg tcgtgtagcg acgggagccg ttgatggact
      721 cagcaggaat cgccagttcg ctggcggagc tcgttcgtgt ggcggaggag gaggggctgg
      781 ctgggctgcg tgtggcccgg aacgggatct ccgtggactt ccgcagatcc gcagccacgg
      841 cggcaacgac gccgctcgta cccgcctcga cggcggagga acccgaacct gaacaggcgt
      901 cgaccgcgcc ggcggccggt gggcacgagc tgcgcagcag gctggtgggc gtgttccgac
      961 gctgccagcc ggggcgggat cggccggtgg tgaaccccgg cgacccggtc cgtgagggcc
     1021 agctgctggg gtacatcgag tcgatgcgcg tgatgaacga tgtgacggcc gatgtcaccg
     1081 ggatcctcga tgagttcctg gtcggggacg gagagggtgt cgactacggc cggcccatcg
     1141 cgcggttccg agtaggctag gaccagggtg accgcgcggg gcggcgacat cgctcgatgc
     1201 caatggcctt cggaggagcc atgcggggcg cacgacctgg gtgcccaagc gacggagcgc
     1261 gggacctgag ggcggtgtca gaggccgggc gtcatgcgcg cgggcagacc atgatcggtc
     1321 tgctcgtgtc gctgatcatc atcctgctgg tgctcctggt tggcctcggc atgatgaaga
     1381 ctcagggcgt ggggaagtcc acaccggctc gggtggagtc gaaggctcga ggtcttcagt
     1441 gccagacgta cctccagcag atgcggatga tggccttgat caacaacgaa gagaagcagg
     1501 aaccgacatc tctggaggac ttccgcggcg ctggcgagct tcggtgcccg gtatccggca
     1561 tgcggtacta cttcagtccc gcgcgtgccg gtgaccctca gacccgaggg ctgtactgcc
     1621 cgtatcccga gcactggaac ttctaggaga aggggcgcgc cggggtccga cacggaggtg
     1681 gtgtcggggc gcgcgacggt atgttcaaga ggatcctggt cgcgaaccgg agcgagattg
     1741 cggtccgcat catccgcgcc tgtcacgagc tgggcatcga ggccattgcc gtctactccg
     1801 aggcagaccg cgaagccctg cacgttcaga tggccgacca ctcggtgtgc attggcccgc
     1861 cggcggcgcg tgagagctac ctcaatgtgc cgaatatcat gtcagccgcc atgattcacg
     1921 ggtgcgatgc cattcatccc gggcttggat tcctggctga gaacgcggat ttcgccgaga
     1981 tatgtgagac ctgcgggatc acattcatcg gccccaccgt cgaggccatc ggcaaaatgg
     2041 gagacaaggc acgcgcacgc cgcatcatgc acgaggcggg ggtgccggtc atccgcggca
     2101 ccagtgaggc cgtggaggac gaagcgagca ttctcaaggc ggcggcggac ttcggctacc
     2161 ccctccgcgt gaaggcgtcg ttgggcgggg gggggcgcgg gatccgtatt gttcacagtg
     2221 aggaagagct actccgcagc gtacagctcg cccgcgctga ggccaaggcg gccttcggta
     2281 gccccgatgt ctatctggaa cg
//
LOCUS       DAOSPL010000772         2301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46733_length_2301_cov_2.631790, whole genome shotgun sequence.
ACCESSION   DAOSPL010000772 DAOSPL010000000
VERSION     DAOSPL010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46733_length_2301_cov_2.631790"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(390..800)
                     /locus_tag="PLQ54_13520"
     CDS             complement(390..800)
                     /locus_tag="PLQ54_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94333.1"
                     /translation="MDEYPVKSTRWAAPGKPVVIEIRATAKYTDEKTRYGGEELDGLM
                     SRGVRVPLPGIGEDWGRVCEGIHARKRTRRSRAGEAVYDEMVTHLRIGESATPVVVLL
                     REVLAIDYDYDREGVHESGRYSISVEGRPKTPSG"
     gene            948..>2301
                     /locus_tag="PLQ54_13525"
     CDS             948..>2301
                     /locus_tag="PLQ54_13525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04083 family peptide-modifying radical SAM
                     enzyme"
                     /protein_id="HOS94334.1"
                     /translation="MPEPAAAHASAPAAPQFMLVPSLACPASCAYCFGPHQGPTMSPQ
                     TLERALDFMARVTPASRPGPVKVTFHGGEPLSAGHELWRQALDGLRDRFGIGGYEVAL
                     QSNLWALDDVFCELFAEHRVEVGTSVDGPEDITDSQRGAGYFARTMAGVGRARARGLD
                     VGCIATFTAASAPRWRVVFDFFVEHQLGWSVHAAVPRLGGPTSSLRLTPAAYRDLLLD
                     LADAYVDRRHDTDISSLNQVFRGVASGEGKVCTFRDCSRMFLAIDPEGGIYHYQRLAG
                     RPEWRLGDLHDEPTSEELYASPVGVRLREREARVADVCGECPHLAYCRGGCPYNAWAS
                     GGVEATIDPYCEAYRAVFDHVQQRLAEEMGSEANIAAVAERPYDGRGHPLLRRGPLID
                     LCKDGPHPRVVARTARRIIAAVEMARGPDDAVVAERLVAMGICRSRTTADASIAGLRA
                     R"
BASE COUNT          349 a          818 c          781 g          353 t
ORIGIN      
        1 acatggagcc gtacatcagg ccgaacatca gagccaggcc cgcaatgccg accacgaagc
       61 gccagatacc gcggcctacc gcggccggcc cccccccgga tgagcgtgac ggcgatccac
      121 acgatcacgc cgaccgccag cacgatcccg gcgcagcagc ctcccatgag acgccagtcc
      181 gtctcgggtg gaccaagcgg ggcgggcgga agcgatggcc gacggaagtg cgctagctcc
      241 tccgctcgac gcctgtcctg ttccgctatc tcccttggcg tgggccgcag ttcagtaagc
      301 agtggcgtac acgggggact ccaactgcgc acaggacacc acctccacgt cggcgccgcg
      361 gcggtcgtcg ctcccccggg gggagtagcc tagcctgagg gcgtcttcgg ccggccctct
      421 acgctgatcg agtaccgccc gctctcgtgg acgccttcgc ggtcgtagtc gtagtcgatc
      481 gccagtactt cgcggagcag gaccaccacg ggagtggccg actcgccgat ccgcaggtga
      541 gtgaccatct cgtcgtacac tgcctcgccc gcccgggacc gacgggtccg cttgcgggcg
      601 tggattccct cgcagacacg gccccagtcc tctccgatgc ctggcaaagg cacccggacg
      661 ccgcgactca tcagcccatc gagctcctcg cccccgtagc gggtcttctc gtccgtgtac
      721 ttggcggtcg cccggatctc gatgaccacg ggcttgcctg gggcagccca gcgcgtgctt
      781 ttcaccggat actcgtccat tcagcacgcc tcccgcgaca agcgtagcat acggctcagc
      841 ttgcgaggga acgcgaagtc tggcgacgaa cagaagcgag gggcaagcgg gcgagcgggg
      901 ggagacacac gactccgtgt cgacgagggg acagccaggg cgagtgtatg cctgagccgg
      961 ccgccgctca cgccagcgcg ccggcggcac cccagttcat gctcgtgcct tcactggcgt
     1021 gccccgcctc gtgcgcttac tgcttcgggc cacaccaggg cccgaccatg tcgccccaga
     1081 cactggagcg cgcgttggac ttcatggcac gcgtgacccc tgcgtctcgg ccaggccccg
     1141 tgaaggtcac cttccacggc ggcgagccgc tttcggctgg ccacgaactc tggcgtcagg
     1201 cgctagacgg cttgcgcgat cggttcggta tcggtggcta cgaggtagcg ctccagagca
     1261 acctctgggc actcgacgat gtcttctgcg agctcttcgc ggaacaccgc gttgaggtcg
     1321 gcaccagcgt cgatgggccg gaggacatca ccgactccca gcgcggcgca ggctacttcg
     1381 cgcggacgat ggcgggggtc gggcgcgccc gggcacgcgg gcttgatgtg gggtgcatcg
     1441 cgaccttcac ggcggcctcg gcgccgcggt ggcgggtggt gtttgacttc ttcgtcgagc
     1501 accagctcgg ctggagcgta cacgccgccg tgccgcgcct gggtgggccc acgagctcgc
     1561 tcaggctgac gcctgccgcc taccgcgacc tgctgctcga tttggccgac gcctatgtcg
     1621 accgccgcca cgacaccgat atcagctcgc tgaaccaggt gttccgcggt gtcgcctctg
     1681 gtgagggcaa ggtctgcacg ttccgcgact gcagcaggat gttcctggcc atcgaccccg
     1741 agggcgggat ctaccactac cagcggctgg ccggacgccc cgagtggcgc ctaggcgacc
     1801 tgcacgacga gccaacgtcg gaggagctct acgccagccc ggttggggta cgcctgcgcg
     1861 agcgtgaggc ccgagtggcc gacgtgtgcg gcgagtgccc acacctagcc tactgccgcg
     1921 ggggatgccc ctacaacgcc tgggcatccg gcggcgtcga ggccaccatc gatccctact
     1981 gcgaagccta ccgcgccgtg ttcgaccacg tgcaacagcg cctcgctgag gagatgggca
     2041 gcgaggcgaa catcgccgcg gtggctgagc ggccctacga cggccgaggg cacccgctcc
     2101 tgcggcgggg gccgctcatc gacctctgca aggacggccc gcatccgcgg gttgtggctc
     2161 gcacggcgcg gcgcatcatc gcggctgtcg agatggcccg agggcccgat gatgccgtgg
     2221 tggcggagcg cctggtggcc atgggcatct gccgctcgcg gaccactgcc gacgcatcga
     2281 tcgctgggct gcgggcacgg c
//
LOCUS       DAOSPL010000773         2301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46735_length_2301_cov_2.431434, whole genome shotgun sequence.
ACCESSION   DAOSPL010000773 DAOSPL010000000
VERSION     DAOSPL010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46735_length_2301_cov_2.431434"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2301)
                     /locus_tag="PLQ54_13530"
     CDS             complement(<1..>2301)
                     /locus_tag="PLQ54_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94335.1"
                     /translation="VSGQGWQTIRIDLLRDANWRQPLSAFRIDLTNDMRAQVAVDWVR
                     LTQGVPAELTGLQTLGAPSGVAAQVTLEVADRSPVAGSDQDVAARVTDAQGDPVAGQP
                     VVIAIREGAGTIAESGTMALKAEGGLRGLTGPDGRFVVRYEAGRQAGSTDELIARTEF
                     PDMGSEIIRATVQAGPPDHYIVLSPEPIIVPEVDTPLLVRAQVADVCGNPLPLEGRKL
                     SWEVAEGQLDGAAPATAADGSATARLVPDMARRWVYTVRVTDDQGLTGQSGPVCVLPK
                     GPRPDRVRLGDNGYFTTGPGQPFVPLGGFYGVWVPGIAAPGQEKGRAIHAFHEATEEE
                     ILDWFGFLQAQGITAIRMMLRTHGPDGTEAMDIGGRVNRRLFATTLRYFDLARRFGLR
                     FMLTLHDDYDKPVYCNARHLKRFALPAFAGEDLNALPAFQRRFIRDQELVAPAERYTD
                     PDAIACQDQYAREIVGYLKDNPVVFSWELENEMVDCPVSWVNHAVDVIREVDPDTPVC
                     VSHGGGGIVTSDPLYWTTQSNVDFYTYHLYPVGMTRPETDYGAAVDLLTRYGRMAGTC
                     FMGESAGDEFSSYPPERDRDRRYVMRDIIWFGLVNGNPGSFFWNARGFEVEEFRLARA
                     LMDRIDWTAFERRRPTIAVLVDHPLDDDKHWRSPGGQAEMRVMEQYVQHFLSLGVDFD
                     FATDAAGYATVCGVAAFEPPALTGGRRVSPGYQLASLTRADGQQGLAYARNFAGIVAW
                     EVKAGQTMWLRQRAKARLVALLGAEL"
BASE COUNT          362 a          819 c          755 g          365 t
ORIGIN      
        1 gaagttcggc gcccagaagg gccaccaggc gtgccttcgc ccgctgccgt agccacatcg
       61 tctgccccgc cttcacttcc caggcgacga tgcccgcgaa gttgcgcgca taggccaacc
      121 cctgttgccc gtcggccctc gtgagactcg cgagctggta gcccgggctg acgcgccgcc
      181 caccggtcag cgcggggggc tcgaaggcgg cgacaccgca caccgtggcg tagcccgccg
      241 catcggtggc gaagtcaaag tccacaccca ggctgaggaa gtgctgcaca tactgctcca
      301 tcacccgcat ctctgcctgg cccccggggc tccgccagtg cttgtcgtca tcgagcggat
      361 ggtcgacgag gacggcgatg gtgggccgcc gacgctcgaa tgccgtccag tcgatccggt
      421 ccatcagcgc ccgagccagc cggaactcct cgacctcaaa gccgcgggca ttccagaaga
      481 acgacccggg gttaccgttc acgagcccga accagatgat gtcccgcatc acgtaccgcc
      541 ggtcgcggtc acgttcgggt ggatacgacg agaactcgtc acccgccgac tcgcccatga
      601 agcacgtgcc ggccatgcgg ccatagcggg tcaggaggtc gacggcggcg ccgtagtcgg
      661 tctcgggcct cgtcatgccc accgggtaca ggtgatacgt gtagaagtcc acgttcgact
      721 gcgtcgtcca atagagcggg tcgctggtca cgatcccgcc gccgccatgg ctcacgcaga
      781 ccggagtgtc ggggtcgacc tctcggatca cgtccaccgc gtggttgacc cagctcacgg
      841 ggcagtcgac catctcattc tcgagttccc atgagaacac aacggggttg tccttgaggt
      901 agcccacgat ctcgcgggca tactggtcct ggcacgcgat ggcgtcgggg tcggtgtacc
      961 gctcagcggg cgccacgagc tcctgatcgc ggatgaaccg ccgctggaag gccggcagtg
     1021 cgttcaggtc ctctcccgcg aaggccggca gcgcgaatcg cttcagatgc cgggcgttgc
     1081 agtagaccgg tttgtcgtag tcgtcgtgca gggtgagcat gaaccgcagc ccgaaccgac
     1141 gggccaggtc gaagtagcgc aacgtggttg cgaacaggcg gcggttcacc cggccaccaa
     1201 tgtccatggc ctcggtgccg tccgggccgt gggtgcggag catcattcgg atggcggtga
     1261 tcccctgggc ttgcaggaag ccgaaccagt cgagaatctc ctcctccgtg gcctcgtgga
     1321 atgcgtggat ggcccggccc ttctcctgcc cgggcgcggc aatgcccggg acccagacgc
     1381 cgtagaaccc gcccaggggc acgaacggct ggcccggccc cgtggtgaag tagccgttgt
     1441 cgcccaagcg aacgcgatcc ggtcgcggcc ccttcggcag cacgcagacc ggccccgact
     1501 gtcccgtcag gccctggtcg tcggtcaccc gtacggtgta gacccagcgc cgggccatgt
     1561 cgggcacgag gcgcgccgtg gcgctaccgt ccgccgcggt cgcgggggcc gcgccgtcga
     1621 gctgcccctc ggccacctcc cagctcagct tgcggccttc caggggcagc ggattcccgc
     1681 acacgtcggc gacctgagcc cgcaccagga gcggcgtatc gacctcggga acgatgatcg
     1741 gctcggggct caggacgatg tagtgatccg gcgggcctgc ttgaacggtg gctcgtatga
     1801 tctcggatcc catgtcgggg aactccgttc gggcgatcag ctcatcggtc gatcccgcct
     1861 ggcgtccggc ctcgtagcgg acgacgaaac gaccgtcggg ccccgtcagc ccgcgtagac
     1921 ccccttcggc cttcagcgcc atggtgccgg actccgcgat cgttcccgca ccctcacgga
     1981 tggcgatgac cacaggctgc cccgccaccg gatctccttg ggcgtcggtc acccgcgccg
     2041 ccacgtcctg gtcgctaccg gctaccggcg atcgatcggc cacctcaagt gtgacctgcg
     2101 ccgccacgcc acttggggcg cccagagtct gcagcccggt caactccgcc gggacgccct
     2161 gcgttagccg cacccagtcc acagcgactt gggcccgcat gtcgttggtg agatcgatcc
     2221 ggaacgcgga gagcggctgg cgccagttcg catcgcgcaa caggtcaatg cggatcgtct
     2281 gccagccctg gccgctgacc g
//
LOCUS       DAOSPL010000774         2300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46754_length_2300_cov_2.802673, whole genome shotgun sequence.
ACCESSION   DAOSPL010000774 DAOSPL010000000
VERSION     DAOSPL010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46754_length_2300_cov_2.802673"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PLQ54_13535"
     CDS             <1..329
                     /locus_tag="PLQ54_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007463646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxylamine oxidoreductase"
                     /protein_id="HOS94336.1"
                     /translation="YIYFELWHHEGRRARHGAAMMGPDYVQWHGFYELSKRFYAEFLP
                     LARELGVNAGAGEDMVGFIDATLRGTEGQDWERYHAWTEGLSPEQSQRLLELEREAYG
                     SSAGTP"
     gene            420..2063
                     /gene="hcp"
                     /locus_tag="PLQ54_13540"
                     /gene_synonym="priS"
     CDS             420..2063
                     /gene="hcp"
                     /locus_tag="PLQ54_13540"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOS94337.1"
                     /translation="MFCYQCEQTAKGAGCTVTGVCGKDPVTAAMQDLLIHVTKGIGAY
                     AHRARELGAADNAIDTFVTEALFTTVTNVNFDAARIRGLIATAVSVRNQARRLYEEAC
                     AKAGQPPEALSGPATFVPAADEAGLLAQAVDVGIGARIEAQGPDLTGLQELLTYGLKG
                     TAAYTDHAQILGRTDESVFAFLHEALSYLAGADQSVDGLLGLCLKCGEVNLTAMGILN
                     AAHIERFGKPQPTPVRVEPVRGKCILITGHDLLDLEALLKQTEGKGINVYTHSEMLPA
                     HGYPGLKKYPHLVGNYGSAWQNQQKEFAAFPGAIVFTTNCLQRPLDSYKDRVFTCGLV
                     GWPDVPHIENRDFTPVIEAALAAPGFTEDGGDQTILVGFGHDAVLGVADRVIDAVKAG
                     AIRHFFLIGGCDGAKPGRNYYTELAQAVPDDCVILTLACGKYRFNKLDFGDIGGIPRL
                     LDIGQCNDAYSAIQIAVALAGAFDTDVNSLPLSLILSWYEQKAVCILLTLLHLGIRNI
                     RIGPSLPAFITPAALQILVDKFNLTPISTPEEDLAAILG"
BASE COUNT          391 a          768 c          759 g          382 t
ORIGIN      
        1 actacatcta cttcgagctg tggcaccacg agggccgccg tgcgcggcac ggggccgcca
       61 tgatgggccc cgactatgtg cagtggcacg ggttctatga gctgtcgaag cggttctatg
      121 ccgagtttct gcccctggcc cgcgagctcg gagtgaatgc cggggccggc gaggacatgg
      181 tgggtttcat cgacgcgacg ctgcggggca ccgagggcca ggactgggag cgctaccacg
      241 cctggactga gggcctctca cccgagcaga gtcagcggct tctcgaactc gagcgggaag
      301 cctacggcag cagcgcgggc accccatagc ggcagacacg gtggcgttgc gccggcagca
      361 gggtacgggc ccggatccgg gctcggcgac caatgaacca ggaggcatct gacgtgagca
      421 tgttctgcta ccagtgcgaa cagacggcca aaggcgcggg ctgcaccgtt acgggggtct
      481 gcggcaagga ccccgtgacc gcggccatgc aagacctgct gatccacgtg accaagggca
      541 tcggggccta cgctcatcgc gcccgggagc tgggcgctgc tgacaatgcc atcgacacgt
      601 tcgtcaccga ggcgctcttc accaccgtca ccaatgtgaa ctttgatgcg gcgcggatcc
      661 ggggcctgat tgcgacggcc gtctcggtcc gcaaccaggc cagacggctg tatgaggagg
      721 cctgtgccaa ggcgggccag cccccggagg ccctgagcgg acccgcgacc ttcgtgcccg
      781 cggctgacga agccggtctc ctggcgcagg ctgtggatgt tggcatcggg gctcgcatcg
      841 aggcccaggg ccccgacctg accggtctcc aggagctatt gacctacggg ctcaagggca
      901 cggcggccta taccgatcac gcgcagatcc tgggtaggac cgacgagagt gtcttcgcct
      961 tcctccatga ggcgctgagc tacctggccg gtgccgatca gtccgtcgac ggcttgctgg
     1021 gcctctgctt gaaatgcggc gaggtgaacc tcacggccat ggggatcctc aatgcggcgc
     1081 acatcgagcg cttcgggaag cctcagccca cccccgtccg agtcgaacca gtccgcggca
     1141 agtgcatcct catcacgggg catgatctgc tggacctcga ggcgctcctg aagcagaccg
     1201 agggcaaggg catcaacgtc tacacgcact ccgagatgct gccggcccat ggttacccgg
     1261 ggctgaagaa gtacccacac cttgtcggca actacggcag cgcttggcag aaccagcaga
     1321 aggagttcgc ggcctttccc ggcgccatcg tgttcacgac gaactgcctc cagcggcccc
     1381 tcgatagcta caaggaccgt gtgttcacgt gcgggctggt cggttggccg gacgtgcccc
     1441 acatcgagaa ccgtgacttc acaccggtca tcgaggccgc cctggcggcg cccggcttca
     1501 cggaggatgg cggcgatcag accatcctgg tgggcttcgg gcacgacgcc gtgctgggcg
     1561 tggctgacag ggtcattgac gccgtcaaag ccggggccat ccgccacttc ttcctgatcg
     1621 gaggatgtga cggtgccaag ccgggccgca actactacac cgagctggcc caggctgttc
     1681 cggacgactg cgtcattctg acgctggcct gcggcaagta ccgtttcaat aagctagact
     1741 tcggtgacat tggcggcatc ccgcgcctgc tcgacatcgg tcagtgcaat gacgcctact
     1801 cggccatcca gatcgccgtg gctctggccg gggctttcga caccgacgtc aacagcctcc
     1861 cgctatcgct gatcctctcg tggtatgagc agaaggccgt ctgcatcctg ctcacgctgc
     1921 tgcaccttgg gatcaggaac atccgcatcg gccccagcct gccggctttc atcaccccgg
     1981 cagcgctgca gatcctggtt gacaagttca acctgacgcc catcagcacg cccgaggagg
     2041 acctggccgc gatcctgggc tgaacggtgg gcgctgagcg aacggaacgt ccctcgcccg
     2101 gcctcgagtc ccggggccgg cttctcccac cgggaggcgg gccgggtgag gggtgctctg
     2161 ggaccggccc agattgcgga cgccacccgc cgcgccccct gctcgttacc ggaagggcgt
     2221 cctgcccgag tcatgccagg gcgaggcgcc acatgcggat ctcgttggtg ttgttcggcc
     2281 gggccacctc ggcgtacacg
//
LOCUS       DAOSPL010000775         2300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46758_length_2300_cov_2.692650, whole genome shotgun sequence.
ACCESSION   DAOSPL010000775 DAOSPL010000000
VERSION     DAOSPL010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46758_length_2300_cov_2.692650"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..1047
                     /gene="thrC"
                     /locus_tag="PLQ54_13545"
     CDS             4..1047
                     /gene="thrC"
                     /locus_tag="PLQ54_13545"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOS94338.1"
                     /translation="MGPSTPIISLGEGATPLVAADRLRAVAGIPPRVLLKCEGTLPTG
                     SFKDRGMTMAISKAVELGARGVMCASTGNTAASAAAYAAKADLPCVVLIPGGAIALGK
                     LSQACTHGARVLAVKGNFDDCLPIVVELTEKGDFALVNSINPYRLQGQKTAAFEICDA
                     LGCAPTYHAIPVGNAGNISAYWMGFTEYREAGVTRTTPRMLGFQAAGAAPMVLGHPVA
                     RPETIATAIRIGNPANWEKARRAIDESGGRIDSVTDDEILEAYEMLARLEGVFCEPAS
                     AASVAGVLKLSRAGYFDGADDAVVTCTLTGHGLKDPDTAMKRAERPKEIEATSEAVLR
                     AVGLGEKMEALTT"
     gene            1059..1946
                     /locus_tag="PLQ54_13550"
     CDS             1059..1946
                     /locus_tag="PLQ54_13550"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOS94339.1"
                     /translation="MSVELANVPKLRSGKVREVFDLGDHLLIVATDRISAFDVIMPTG
                     IPDKGAVLTQISNFWFDKLRDVVPHHVVATHVGDFPAVVREDPRSRDLAGRSVLVRKA
                     EVLPVECIVRGYLAGSGWKDYMATGMTSGVELPPGLQLASRLPEPVFTPSTKAHHGHD
                     ENITRSEAARMIGEELAERIEDVAKRLYLAAREHAEACGLILADTKFEFGLCDGELLL
                     VDEALTPDSSRFWDAELYRLGESPASFDKQFIRDYLEGLDWNKQAPGPVLPDDIVART
                     REKYLEAFRRLLGREFTAA"
     gene            1981..>2300
                     /locus_tag="PLQ54_13555"
     CDS             1981..>2300
                     /locus_tag="PLQ54_13555"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS94340.1"
                     /translation="MLPLQVAEYIARYGLFAPGEAVLVAVSGGPDSVALLRCLSDLGG
                     RWGLGLSVGHVNHQLRGEESDQDEAFVADLARRLGVAFATRRVDVRSRAAAHGSRPAG
                     DAGRV"
BASE COUNT          370 a          709 c          808 g          413 t
ORIGIN      
        1 cccgtgggcc cctcgacgcc gatcatcagc ctgggtgagg gggccacgcc gctcgtcgcc
       61 gccgaccggc tccgggccgt agcgggcatc ccgccgcgcg tcctgctcaa gtgcgagggg
      121 acgctgccca cgggctcgtt caaggaccgt ggcatgacca tggccatctc caaggcagtg
      181 gagctcggcg ctcgaggggt gatgtgcgcg tcaacgggca acacggcggc gtcggctgca
      241 gcctacgcgg ccaaggcgga tctgccctgt gtggtgctca tcccgggcgg ggccattgcc
      301 ctcggcaagc tgtcgcaggc atgcactcat ggagcccgag tcttggcggt aaagggcaac
      361 ttcgatgact gtcttcccat tgtggtcgag ctgaccgaga agggcgactt cgcactggtg
      421 aactcgatca atccctaccg gctccagggg cagaagacgg cggcgttcga gatctgcgac
      481 gcgctggggt gtgcgcccac ttaccatgcc atccccgtgg gcaacgcggg gaacatcagc
      541 gcctactgga tgggtttcac cgagtacagg gaggccgggg tgacgcgtac caccccgcgg
      601 atgctggggt ttcaggccgc tggggcggcg ccgatggtcc tgggccaccc cgtggcccgg
      661 cctgagacca tcgcgacggc catccgcatc gggaaccccg ccaactggga gaaggcgcgc
      721 cgagccatcg acgagtccgg cggccgcatc gactcggtca ccgatgacga gatactggag
      781 gcctatgaga tgctcgcccg gctcgagggg gtgttctgtg agccggcatc ggcggcgtcg
      841 gttgcgggag tcttgaagct ctcacgtgcc ggatacttcg acggcgccga cgacgcggtg
      901 gtcacctgca cgctgacggg ccatgggctc aaggacccgg acacagccat gaagcgtgcc
      961 gaacgcccca aggagatcga ggccacctcg gaggcggtac tccgagcggt gggtctggga
     1021 gagaagatgg aggccctaac gacgtgaccc gcccgatcat gagtgttgag cttgcgaatg
     1081 tgccgaagct gcgttcgggc aaggttcgcg aggtgttcga cctcggtgat catctgctga
     1141 tcgttgccac ggaccggatc tcggcctttg acgtcatcat gcctaccggc atccctgata
     1201 agggcgcggt actgacccag atatcgaact tctggtttga caagcttcgc gacgtggtgc
     1261 cccaccacgt cgtggcaacc catgtcggtg acttccctgc cgtggttcgc gaggaccctc
     1321 gctccagaga cctggcgggt cggagtgtgc tcgtgcgcaa ggccgaggtc cttccggtcg
     1381 agtgtattgt ccgaggctac ctggcgggct ccgggtggaa ggactatatg gcgaccggca
     1441 tgacttcggg agtcgaactg ccgccaggac tgcagctcgc gagccgtttg cccgagccgg
     1501 tcttcacacc gtcgaccaaa gctcaccacg ggcacgacga gaacattacc cgcagcgagg
     1561 cagctcgaat gatcggtgag gagctggccg aacgcatcga ggatgtggcg aagcgcctct
     1621 atctggcagc ccgggagcat gccgaggcct gcggcctcat cctggccgac accaagttcg
     1681 agttcgggct ctgtgacggg gagctgcttc tggtcgatga ggcattgacg ccggattcat
     1741 ctcggttctg ggatgccgag ctgtaccggc tgggtgagtc gccggccagc tttgataagc
     1801 agttcattag agattatctt gaggggctgg actggaacaa acaggcccca ggccccgtgc
     1861 ttcccgacga catcgtcgcc cggactcgcg agaagtacct ggaggcgttc cgcaggctgt
     1921 taggtcgcga gttcaccgcg gcgtgaggtg gtcggggagg cgagtcaccg ctccagcacc
     1981 atgctgccac ttcaggttgc cgagtacatc gcgcgctatg gtttgtttgc gccgggtgag
     2041 gccgtcctgg tggctgtctc cggagggcct gactcggttg cgcttctgcg gtgcctgtcg
     2101 gacctcggcg gccggtgggg gctcggcctc agcgtgggcc acgtcaacca ccagcttcgg
     2161 ggtgaggagt ccgatcagga cgaggccttt gttgcggacc tggctcgccg gctcggcgta
     2221 gcgttcgcca cgcggcgcgt ggatgttcga agccgggcgg cggctcatgg atcgcgtccg
     2281 gctggcgacg ctggccgtgt
//
LOCUS       DAOSPL010000776         2298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46807_length_2298_cov_3.375390, whole genome shotgun sequence.
ACCESSION   DAOSPL010000776 DAOSPL010000000
VERSION     DAOSPL010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46807_length_2298_cov_3.375390"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="PLQ54_13560"
     CDS             <1..1287
                     /locus_tag="PLQ54_13560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS94341.1"
                     /translation="TWTGYHVAELESTLDELVEAGVLRRTGNILRITDDPELRRGLEQ
                     VSRYYHTARGAVEELVARLHEDHQRMAEQLERLEVSLDVIVHTMADGLIVTNADRCVV
                     LVNPGAERLLGLGEADRVGRPALEVFAGTPVEPLIGRLDEVGPGSQLQTELRVIVDDE
                     PRYVRANVRPVGTDEGKIQGHVVLLSDITDLRRLDDLKSELISFVSHELKAPLTAVKG
                     YAGSLLSTSLNLGKETQEEFLREIDGEVDRLSRLIDSFLDISKLDAGRPIEIMPVSFA
                     LEDLIRQAVQIQLAVSNRCIPKVEVPEDLGHVTADRDKVLQVLTNLLSNAVKYSPAGR
                     DVVVSAERVPGAVRVQVADQGLGMSPEQIENLFVPYYRIRDAKRKAIRGTGLGLYLSK
                     HLVEAHGGEIGCESEEGIGSKFWFTIPDPSPQEAVH"
     gene            1284..>2298
                     /locus_tag="PLQ54_13565"
     CDS             1284..>2298
                     /locus_tag="PLQ54_13565"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HOS94342.1"
                     /translation="MRVLHFDCAAGASGDMILGALADLGVDLPAIAHRLKHDLSIDFD
                     LAVERGNEGALRATRVTVSLPGGETKQGHRHRDHEHDHVSDHGHGHDDSRGHEHGHEH
                     GHEHGHGHGHGHEHEHGHMSYADLRDIIGRGALPERVRERSLSVLRAIAEAEATVHGT
                     PVEAVRFHELGGIDSLVDICGAMLALEELEVERCTAGRLPVSHGFVRCAHGLLPVPAP
                     AVAELMRGLPVSHIDVDGETLTPTGAGILKGVCDVFGSLPDMTVRAVGYGAGQRPGRE
                     VPNLLRVFYGETHEDSPPEGVVSIEANIDNTSGEALGYVVERLLGAGALDVYFTPIFM
                     KKNR"
BASE COUNT          397 a          652 c          838 g          411 t
ORIGIN      
        1 acgtggaccg ggtaccacgt ggccgagctc gagagcacgt tggatgagct ggtcgaggct
       61 ggggtgctga ggcgcaccgg gaacatcctg cgcatcaccg acgaccccga gcttcggcga
      121 ggcctggagc aggtgtcgcg ctactaccac accgcccggg gcgccgtgga agaactggtt
      181 gcccggcttc acgaagacca ccagcgcatg gcggagcagt tggagcgcct ggaggtctcg
      241 ctggacgtga tcgtccacac gatggcggat ggactcatcg tcacgaacgc cgaccggtgc
      301 gtggtgttgg tgaacccggg ggccgagagg ctgctgggcc tgggtgaggc ggatcgtgtt
      361 gggcggccag cgctggaggt gttcgcgggg acacccgtgg agccgctgat tgggcgcctt
      421 gacgaggtgg ggccgggctc acaactgcag accgagctcc gggtgatcgt tgatgacgag
      481 ccgaggtacg tgcgggcgaa cgtgcggccc gtggggaccg atgaggggaa gatccagggg
      541 catgtggtgc ttctgtcaga catcaccgat ctgcggcgtc tggatgatct caagagcgag
      601 ctcatctcat tcgtatcgca tgagctgaag gcgccactca cggcggtcaa aggttacgcg
      661 gggtcgctgc tgtcgacgtc gttgaacctg gggaaggaga cccaggagga gtttctccgc
      721 gagatcgatg gtgaagttga ccgcctgagc cgtctgatcg atagtttcct agacatctcg
      781 aaactagatg ctgggcgccc catcgagatc atgcccgtat cgtttgcact ggaggatctg
      841 atccgccagg cggttcagat ccagcttgcg gtgtcgaacc gctgcatccc gaaggtcgag
      901 gtgccggaag acctgggcca tgtgaccgcg gatcgtgaca aggtgctgca ggttctgacc
      961 aacctgctca gcaacgccgt caagtactcg cctgcgggcc gggatgtggt cgtctcggct
     1021 gagcgtgtgc cgggagcggt gcgcgtgcag gtcgccgacc agggcctcgg gatgtcaccg
     1081 gagcagatcg agaatctgtt cgtgccctac taccggattc gggatgccaa gcgcaaggcc
     1141 atccggggca ccgggttggg gctgtacctc tcgaagcatc tggtcgaggc ccatggtggg
     1201 gagatcgggt gcgagagcga agaagggatc ggatcgaagt tctggtttac catccctgac
     1261 ccaagtcctc aggaggctgt tcattgaggg ttctgcactt tgactgcgca gcgggcgcat
     1321 cgggcgacat gatcctgggc gccctggccg acctgggagt ggatctgccg gccatcgctc
     1381 atcggctgaa gcatgacctg tccatcgact tcgacctggc ggtagagcgg ggcaatgaag
     1441 gggccctccg tgccacgcgg gtgacggtgt ccctgcccgg cggcgagacg aaacaaggcc
     1501 atcgtcaccg cgaccacgag catgaccatg tgagcgacca cggacacggc catgacgatt
     1561 cgcgtgggca tgagcacggg catgagcacg ggcacgagca tgggcatggg catgggcacg
     1621 ggcacgagca cgagcacggg cacatgtcgt acgccgatct gcgggacatc attggccgcg
     1681 gcgcgcttcc cgagcgggtg cgcgagcgat cgctgagcgt tctgcgcgcc atcgccgagg
     1741 ccgaggccac ggtccacggg acgccggtgg aggcggtacg cttccatgag ctgggtggga
     1801 tcgactcatt ggttgatatc tgcggtgcga tgctggcgct ggaggagttg gaggtcgagc
     1861 ggtgcaccgc gggtcggctt ccggtgagcc atgggttcgt tcgttgtgcc catggcctgc
     1921 ttccggttcc cgcgcctgcc gtggccgagc tgatgcgtgg gctgcccgtg tcgcacatcg
     1981 atgtcgatgg tgagacgctc acgccaacgg gtgcgggcat actcaagggc gtgtgcgacg
     2041 tttttgggag cttacccgac atgacagtcc gggcggtggg ctatggtgcg gggcagagac
     2101 ccgggcgcga ggtgccgaac ctgctacgcg tgttctacgg cgagacccac gaggattcgc
     2161 cgcccgaggg tgtcgtgagc atcgaggcca acatcgacaa cacctcgggc gaggccctgg
     2221 gttacgtggt cgagcgcctg ttgggcgcgg gagcactcga tgtctacttc acgccgatct
     2281 tcatgaagaa gaaccgtc
//
LOCUS       DAOSPL010000777         2297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46843_length_2297_cov_2.081624, whole genome shotgun sequence.
ACCESSION   DAOSPL010000777 DAOSPL010000000
VERSION     DAOSPL010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46843_length_2297_cov_2.081624"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PLQ54_13570"
     CDS             complement(<1..562)
                     /locus_tag="PLQ54_13570"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOS94343.1"
                     /translation="MVGALVLLVLACGVAVFAQWRTALRPVDPADTRHIRVQIDPGTG
                     ARGIAERLERAGVIRSALWFRLAAARSRQDVSFKAGDYVFQPSMRPTEIMRDLVAGRS
                     VLVPVTVPEGYTLRQIADRLSDAGVVDANDFLAYATQAGPAHISGIAVPAKSLEGYLF
                     PDTYFFREGEKPAAICATPARPCQGLP"
     gene            complement(701..1531)
                     /locus_tag="PLQ54_13575"
     CDS             complement(701..1531)
                     /locus_tag="PLQ54_13575"
                     /inference="COORDINATES: protein motif:HMM:NF019358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94344.1"
                     /translation="MSAPTGGGTEPTAPVPATPVVAPTPATTPTTPPAPVTYAGPVKN
                     LAAQSAGGTVMASTFVNEEFSNARLNNGIRGVDADEEVWTSAASPKYPHDLVASFANG
                     EVHLVSKVVVCGPGMLELFGDRMPKDVEVLVSTTDTNEESFTKVASTQLKMVSEDQAI
                     EFPPVAAKYVKLRILSNHGAPRFVELSELEVWGDPAPVTAAAAAPAAPVTAPTEATPA
                     QAPPAPAVTEPVPPEESATTVPAVPTDIQDPEQLDKLIRDIEDVLARLRALRQTQSGG
                     "
     gene            1737..>2297
                     /locus_tag="PLQ54_13580"
     CDS             1737..>2297
                     /locus_tag="PLQ54_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94345.1"
                     /translation="MVATGDGGVVPTGGVGNAGVTGGTAPGVGVATAGAAPSGITGPG
                     VGAVTGAGAGAGAGPRPLAPPGPGATAPGIAGVLGTVTGGGGAIVTPVGGTGPATGAF
                     GSGVVLMMGPVPPGAVPAPGGVLGSAGPASPPVTPVGSAGVGVAGSAGGADGGVAISL
                     AGVAVAAGTTSAAGPVPLAYTSTSASS"
BASE COUNT          305 a          754 c          885 g          353 t
ORIGIN      
        1 acggcagccc ctggcagggg cgtgcgggcg ttgcgcaaat ggctgccggc ttctcgcctt
       61 cgcggaagaa gtaggtgtcg gggaacagat aaccctcgag ggacttggcc gggacggcaa
      121 tcccgctgat gtgggcaggc cccgcctggg tggcgtaggc gaggaagtcg ttggcgtcga
      181 cgacgcccgc atccgacaga cggtccgcga tctgccggag tgtatagccc tccggcacag
      241 tcacggggac gagtaccgac cggcccgcga ccaggtcccg catgatctca gtgggccgca
      301 tggaaggctg gaagacgtag tcgccggcct tgaacgacac atcctggcga gagcgggcgg
      361 ccgcaaggcg gaaccatagg gcactgcgga tcacccccgc ccgttcgagg cgttcggcga
      421 ttccgcgcgc tccggtgccg gggtcgatct gcacccggat gtgccgggta tcggccggat
      481 cgacgggccg cagggcggtg cgccactggg cgaacacggc cacgccacat gcgagcacca
      541 gcagaacgag tgcgccgacc actccgatgc ctcggcgtgc cgcgcgagtc aaagcaccac
      601 tcacttcctc ggggccggcc cgagcccgca tgacgcttcg aacgcagcac gcgggcggct
      661 gagcgcccgc gtgcatggtc tgtgaccgcc attcgatggg ctagccgcct gactgggtct
      721 gacgaagagc cctgagccga gccagcacgt cttcgatatc ccggatcagc ttgtcgagtt
      781 gctccgggtc ctgaatatcc gtcggtaccg cgggcacggt ggtcgcagac tcctccggcg
      841 gtaccggctc cgtcacagct ggcgcaggtg gtgcctgcgc cggcgtggct tcggtcggcg
      901 cggtcaccgg ggccgcggga gccgccgcgg cggcggtgac gggggcagga tcgccccaga
      961 cctccagctc cgagagttcc acgaaccggg gggcgccatg gttgctgagg atccggagtt
     1021 tcacgtactt ggccgcgacc ggcgggaact cgatggcctg gtcttctgag accatcttga
     1081 gctgcgttga ggccaccttc gtgaatgact cttcattggt atcggtcgtc gagacgagga
     1141 cttccacatc cttgggcatc cggtcgccga acagctcgag catccccggg ccgcacacga
     1201 cgactttgga gaccaggtgc acttcaccat tggcgaatga tgccacaagg tcgtgcgggt
     1261 acttggggct ggcggcggag gtccacacct cttcgtcggc gtcgacgccg cggatgccgt
     1321 tgttgagccg cgcgttgctg aattcctcgt tgacgaaggt ggacgccatc accgtcccac
     1381 ccgccgattg ggccgccagg ttcttcacgg ggccggcata ggtgactggg gccggcggag
     1441 tcgtgggcgt ggtcgcaggt gtcggagcca ccaccggcgt cgcgggcacc ggcgcagtcg
     1501 gctcggtgcc gccccccgtg ggcgcactca cggccgcctc acctgccggt cccggtgtcg
     1561 gtgcgatggg cgtggccggc tctatggtcg gcgtgggttc ggtctccgaa cccgggtcca
     1621 cgaaagccgg ggcgtcagcg ggcgcgactg ccagtgactc ggttggcggc tgcggggcgg
     1681 tgggactggg ctgcgggacc tcgggagcca cggtggtgac ggagggaggc actggcgtgg
     1741 tcgccaccgg cgatggtggc gtggtcccga ccggaggtgt tggcaacgcg ggggtgacgg
     1801 gggggacggc ccctggcgtt ggcgtggcca cggcaggagc ggctccctcc gggatcaccg
     1861 gcccaggggt gggggcagtc acgggtgccg gggccggggc tggcgcgggg ccgaggccgc
     1921 tggcgccccc cgggcctggg gctaccgcgc ccggcatcgc cggtgtgctg ggcaccgtga
     1981 ccggcggggg cggcgcgata gtgacgcccg tggggggcac tgggccagcc acgggagcgt
     2041 tcgggtccgg cgtggtgctg atgatgggtc ccgtgcctcc cggagccgtc cccgcgcccg
     2101 gcggcgtgct gggcagcgca gggcccgctt cgcccccggt gaccccggtg ggcagtgccg
     2161 gtgtgggcgt cgccgggagt gctggtggcg ccgacggtgg cgtggctatc tcgctagcgg
     2221 gcgtggccgt ggccgcgggg accacttccg ccgcgggacc ggtgcccctg gcgtacacct
     2281 ccacctcagc cagctcc
//
LOCUS       DAOSPL010000778         2296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46866_length_2296_cov_3.184293, whole genome shotgun sequence.
ACCESSION   DAOSPL010000778 DAOSPL010000000
VERSION     DAOSPL010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46866_length_2296_cov_3.184293"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLQ54_13585"
     CDS             complement(<1..421)
                     /locus_tag="PLQ54_13585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOS94346.1"
                     /translation="MPRFLLQMECEAGGEVALRGADVRHVTRVLRLGPGDTLEASMPS
                     GLWASLRIEWAGPAEVRARVLSIRPAPGDPTHEVILYVALAKGDVIDRIVQRVSELGV
                     TRVVPFACQRSVPTPDASRIHTRVGRWQRIAEESCKQS"
     gene            complement(544..1332)
                     /locus_tag="PLQ54_13590"
     CDS             complement(544..1332)
                     /locus_tag="PLQ54_13590"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HOS94347.1"
                     /translation="MASEREPLALFAWRPTRMAALYMAGGLAVGAAIVLVGVYVAGQD
                     WGDRGLSASAKLVGWRINLMAGLPWWVAIFLSNLLALVYAIYLGPLGCALEMHARDRW
                     PRYRRMAVRSPLMDRLTGLLSRRLRPLTGTPAHDGVVIAYVVPLLFMVANGAVLGAVM
                     TIFGLRGTTALLRTVAEIAPHGVLEIPALCLAGGLGILAADRVADAAEAGEDIGRAAM
                     AHVRQPALARSALILVAVLLAAGFVEGEITPRVSEWLSPVAGGG"
     gene            complement(1332..>2296)
                     /locus_tag="PLQ54_13595"
     CDS             complement(1332..>2296)
                     /locus_tag="PLQ54_13595"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOS94348.1"
                     /translation="FAYFSFLGLAWAEGRVEPIETDLPEGAVAGTSAVTTVGLMRTYY
                     DADGRIVSAALPMNIRLKAEPRADAQDLGSEEYVPPLALGTGFGGKSSRVIRRPSQVT
                     RMVAVLKGLKAGSVPEVEGRQQVSETEEGGIRVDVRADDVRRAEGQAPALPVAGPDPR
                     WLAAEPFIEVGTPAIKKATAEALAGEVNSARAATRIAEWVLANIEFEDELGPIRSAED
                     VLALGRGVCRDFANLYVAMARTAGLPSRVAQGLVYAQGGFYLHVWAESYVGRWVPVDP
                     TRAHDPVDATHITLSLGTVSDAWDTTRYEGDLTVDITRVEPEDE"
BASE COUNT          372 a          834 c          768 g          322 t
ORIGIN      
        1 cagactgctt gcacgactcc tcagcaatgc gctgccagcg gccgacacga gtatggatgc
       61 gagaggcgtc cggtgtggga accgaccgct ggcacgcgaa cggcaccacg cgggtcacgc
      121 cgagctccga cacgcgctgg acgatgcggt cgatgacatc acccttggcc agggccacgt
      181 agaggatgac ctcatgggtc ggatcgccgg gcgccggccg gatgctcagg actctcgccc
      241 tgacctccgc gggcccggcc cattcaatgc gcagcgaggc ccacagaccc gacggcatgc
      301 tggcctccag tgtgtcaccc ggcccgaggc gcaggacgcg ggtcacgtgg cgcacgtcgg
      361 ccccgcgaag ggcgacctcg ccgcccgcct cgcattccat ctggaggagg aacctgggca
      421 tggtgtctcc tgttgggatg gccatcgtcg caggcagagg gcgaaaggag tcaggagcct
      481 cttccgtgct gaggcgcgcc gagggggatc ctgacgcctg ttcctctggc ccattggtct
      541 ctcctacccc ccgccagcca cgggggatag ccactctgac acccggggcg tgatctcacc
      601 ctcgacgaag cccgcggcga gcaacacggc caccaggatg agcgcactcc gggccagagc
      661 gggctgccgc acatgagcca tggctgcccg tccgatgtcc tctcccgcct cggccgcatc
      721 cgcgactctg tcggcggcga ggatcccgag ccctccggcc aggcacaggg ccgggatctc
      781 gaggactccg tgtggagcga tctcggcaac cgtccgcagg agcgctgtgg taccgcggag
      841 gccgaagatg gtcatcacgg cccccaggac cgcaccgttg gcgaccatga acagcagtgg
      901 aaccacgtag gcgatgacaa cgccatcgtg ggcgggagtg cccgtcagcg gccgcaatcg
      961 gcggctcagc aggcccgtca gacggtccat cagaggcgag cggactgcca tcctccggta
     1021 gcgcggccat cggtcgcggg cgtgcatctc gagggcgcag ccgagcggcc ccaggtagat
     1081 ggcgtagacc aatgccaaca ggttcgagag gaagatggcg acccaccatg ggaggccggc
     1141 catgaggttg atccgccatc ccacgagctt ggccgatgcg ctcaggcccc ggtcgcccca
     1201 gtcctggccg gccacgtaga cgcccaccag cacgatggct gcgccgacgg ccaggccgcc
     1261 cgccatgtag agggccgcca tccgcgtcgg gcgccaggcg aagagggcca gaggctcccg
     1321 ctcgctcgcc atcactcgtc ctccggctcg acccgcgtga tgtcgacggt caggtcgccc
     1381 tcgtaccgcg tcgtgtccca ggcgtcggac acggtcccca agctgagcgt gatatgggtg
     1441 gcgtcgacgg gatcgtgcgc gcgtgtgggg tccaccggta cccagcggcc gacgtagctc
     1501 tcagcccaga cgtgaaggta gaacccgcct tgcgcgtaga ccaggccctg tgccacgcgc
     1561 gagggcagcc ccgccgtgcg tgccatggcg acatagaggt tggcgaagtc gcggcagacg
     1621 ccccggccca gggccagcac atcctcggcc gaccggatgg ggccaagctc gtcctcaaac
     1681 tcgatgttcg cgaggaccca ctcagcgatc cgcgttgccg ctcgggcgct gttgacctcg
     1741 cccgccagcg cttcggcggt cgccttcttg atcgccggag tcccgacctc gataaagggc
     1801 tcggccgcga gccaacgcgg atcggggccc gccaccggga gcgccggcgc ttggccttcg
     1861 gcccggcgga cgtcatcggc ccgcacgtcc acccgaatcc cgccctcttc cgtctccgac
     1921 acctgctgtc ggccctcgac ctcgggtacc gagccagcct tgaggccctt gagcacggcc
     1981 accatgcgcg tgacctggct cgggcgacgg atcacgcggc tgctctttcc cccaaagcca
     2041 gtccccagcg ccaacggcgg aacatactcc tccgagccca gatcctgcgc atcggcccgg
     2101 ggctcagcct tcaggcggat gttcatgggc agcgcggccg acacgatccg gccatcggcg
     2161 tcgtagtagg tgcgcatgag cccgacggtc gtgaccgcac tagtgccggc cacggcgccc
     2221 tccggcagat ccgtctcgat gggctcgacg cgcccctcgg cccaggccag gccgagaaag
     2281 ctgaagtagg cgaact
//
LOCUS       DAOSPL010000779         2296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46868_length_2296_cov_3.090585, whole genome shotgun sequence.
ACCESSION   DAOSPL010000779 DAOSPL010000000
VERSION     DAOSPL010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46868_length_2296_cov_3.090585"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PLQ54_13600"
     CDS             complement(<1..559)
                     /locus_tag="PLQ54_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosidase"
                     /protein_id="HOS94349.1"
                     /translation="MGLDDPSPRFSWQLDDSRRGARQTAYQILVAPSVQDLQRDQGTL
                     WDTGKQDSARSVFVAYRGAPLRSKQRCYWKVRVWDQHGLPTGWSEPSMFEMGIMDVSE
                     WQARWIGLRQGLDEFAMGPWIWHPADQGAGATIYLRAPFVVRADAQVAEARLYATADN
                     GATLFLNNERLGSSQDWKTLSTWDVT"
     gene            960..>2296
                     /locus_tag="PLQ54_13605"
     CDS             960..>2296
                     /locus_tag="PLQ54_13605"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcineurin-like phosphoesterase C-terminal
                     domain-containing protein"
                     /protein_id="HOS94350.1"
                     /translation="MASGTVYLDADADGQRDPEEAGLPGVRVSNQRDIVVTDAEGRWQ
                     LPVDDDTTLFVIKPRGYRTPVDANGIPRGYYQHKPAGSPKSKFPGVAPTGALPASVDF
                     PLGRSPEPDCFTVAVLGDTQTRDAREVGYTAAAVVPDVAGAGPAFGVVLGDVVFDNLS
                     VTEPLVEVMGHAGFPWYYVIGNHDENYDADGDAHADESFERVFGLSYSSFDYGPVHFI
                     ALDDIMWHPKAGETNAHYTSGLGDEQLAFVRNDLALVPEEQLVVLLMHIPIDEMAERQ
                     ELFRLLEGRPHTLSFSAHTHSMEHRFLGEADGWHGKAPHHHVVNGTACGCWWGGAPDD
                     AGIPHATMADGTPNGYSLVTFDGADYSIEYRVFRRSPDYQFRVMVPDEVARADVGSTP
                     IRVNVFAGSERTQVEMRIGTTGPWTALERVSKPDPSYVALRERDKSIEAPYHRL"
BASE COUNT          378 a          766 c          780 g          372 t
ORIGIN      
        1 cggtcacgtc ccaggtcgat agcgtcttcc agtcctggct cgagccgagg cgttcattgt
       61 tgaggaagag cgtcgcgccg ttgtcggccg tggcgtagag tctggcctca gcgacttggg
      121 cgtcggcgcg gaccacgaac ggcgcccgta ggtagatcgt cgcaccagcg ccctggtcgg
      181 ccgggtgcca gatccacggc cccatggcga actcatccag cccctgccgg aggccgatcc
      241 agcgggcctg ccactcgctc acgtccatga tgcccatctc aaacatggag ggctcggacc
      301 acccggtggg cagaccatgc tggtcccaca cgcggacctt ccagtagcac cgctgcttcg
      361 accggagcgg cgcgccgcgg taggccacga aaacggaccg ggctgagtcc tgcttgccgg
      421 tatcccacaa ggtgccctgg tcccgctgga gatcctggac cgagggggcc acaaggatct
      481 ggtaggccgt ctgccgggcg ccacgccgtg agtcatcgag ctgccaagag aaccgcggcg
      541 aggggtcatc gaggcccatg ggagccgcct cgtactcaca acgaaggcac gtgggcggtc
      601 cggccggtga ggggcgcgtc ggcgacgacg cgcccagcag gggcagagcg agtacgacgc
      661 ttccgagcca tagtacggat cgcgacatac ggatagtcct ttctcacctg ggcaacgagc
      721 ttcgaggtcc ggggcatcca cccccaggca ggacaccggg ctgcccgcgc gaaagggccc
      781 ctccggagcc cggtggggtc ggcccgccgc gggccccgac ggaaagggga accacgatca
      841 tgcccacgaa gcgtactcga gcgttccatc aacgcccatg gctgcctggc gtactggcgg
      901 ccctcttcgc agcggccggg tcgccgtgga tcggccccgt cctcgctggt gagacggcca
      961 tggcctctgg cacagtgtat ctggatgctg atgccgacgg ccagagggat cccgaggagg
     1021 ccgggttgcc cggcgttcgt gtgtccaatc agcgggacat cgtggtcacc gacgcggagg
     1081 gccgttggca gcttccggtg gacgacgaca cgacactgtt cgtgatcaag ccgcgcggct
     1141 accggacgcc ggtggacgcc aacgggatcc cgcgcggcta ttaccagcac aagcccgcgg
     1201 gctccccgaa gtcaaagttc cccggagtgg cgccgaccgg cgcactgccc gcatcagtcg
     1261 acttcccgct cggtcgctcg ccggagcccg attgcttcac cgtggccgtg ctcggtgaca
     1321 cgcagacgcg cgacgctcgc gaggtgggct acacggcggc ggccgttgtc cctgacgtcg
     1381 ccggtgccgg gccggccttc ggagttgtgc tcggcgacgt cgtgttcgac aacctctcgg
     1441 tgactgagcc cctggttgag gtgatgggcc atgctggctt cccctggtac tacgtcattg
     1501 gcaaccatga cgagaactac gacgccgatg gcgatgcgca tgctgacgag tccttcgagc
     1561 gcgtgttcgg cctgagctac tcctcgttcg actatggccc ggttcacttc atcgcgctcg
     1621 acgacatcat gtggcacccg aaggccgggg agaccaatgc tcactacacg tccggtctgg
     1681 gggacgagca gctcgctttc gtccgcaatg acctggcgct ggtccccgag gagcagctcg
     1741 ttgtgctgct gatgcacatc ccgatcgatg agatggcgga gcgccaggag ctgttccgcc
     1801 tgttggaggg acggccgcac acactatcgt tctcggccca tacacactcc atggaacacc
     1861 ggttcctcgg cgaggccgat ggctggcacg ggaaggcgcc gcatcaccac gtggtcaacg
     1921 gcacagcttg cgggtgctgg tggggcggtg cgcccgacga cgcgggcatt ccgcacgcga
     1981 ctatggccga cggcactccc aacggatact cgctggtgac cttcgatggg gcggactact
     2041 cgatcgagta ccgcgtgttc cgccgctctc ccgactacca gttccgggtg atggtgcccg
     2101 acgaggtggc ccgggcggat gtgggctcga cgccgatccg tgtgaacgtg ttcgcgggat
     2161 ccgagcggac acaagtggag atgcgcatcg gcaccaccgg accgtggacg gcgctggagc
     2221 gcgtcagcaa accggatcca agctacgtgg cgcttcgcga gcgcgacaag tcgatcgagg
     2281 cgccttacca ccgact
//
LOCUS       DAOSPL010000780         2294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46909_length_2294_cov_3.692720, whole genome shotgun sequence.
ACCESSION   DAOSPL010000780 DAOSPL010000000
VERSION     DAOSPL010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46909_length_2294_cov_3.692720"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..1261)
                     /locus_tag="PLQ54_13610"
     CDS             complement(200..1261)
                     /locus_tag="PLQ54_13610"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS94351.1"
                     /translation="MTSRERVLLALDHKEADRIAITDGPWGTTIARWHREGLPEDKSP
                     HEYFGFEFGGTGCDGSLQLPSEVIEDTPEYSIARNADGATVKNWKSSTSTPELLGFAI
                     NTRAKWEEHRDRMAWNDTRVDWDNALKSHNAGREAGLFCPYAAAIGYDRTAMLVGPED
                     LLMAFVEDPDWVRDMFRANAELNINAMEEMMARGFVFDGVFFYDDMGYKHRPFFSPRA
                     YREVLMPYHKRLCDFAHGRGLKVILHSCGNVNQHVPALIEAGFDCLQPLEVKAGMDLV
                     QLKKDYGDVLALMGGIDVRAMADPDPAVIEREIATKIPIAKKGGGYIYHSDHSVPDNV
                     SFAQFCRTIELVLKYGTYT"
     gene            1461..>2294
                     /locus_tag="PLQ54_13615"
     CDS             1461..>2294
                     /locus_tag="PLQ54_13615"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOS94352.1"
                     /translation="MRIAALTQYGLPPALIPVLEHRFGADLLEVQSRAVAEHGLLEGR
                     SLVVCAPTGSGKTLIGELAALRAATHGRRGIILVPTRSLAEEKWHDLSAAYEPLGLRC
                     LLSTGDRRTTDRDVIRGAFSLAVTVPEKLLALLAAGPSRWLEGAALVVDELQLLADPG
                     RGAALEDLLTRLRARGVPEQVVGLSAVLRGADGLAEWLGAEVVRGAMRPVELRKGVLG
                     DGVFYYREHNSGLWGEERLVPEGWQGGLDELDTGDGKRQDLLTCALSLASSGESVLLF
                     VR"
BASE COUNT          345 a          689 c          852 g          408 t
ORIGIN      
        1 cccgtatgcc agggggtcgg cggcatccag gccgggacgc gcgccgtccg gcagccggct
       61 caccggaccc cgatggctgt tgtctggcat aggctgaagc atccttcgat ggcttggctc
      121 cggaggcgga cacggaccgc cgtgcgtcgg ggcggggcct accccgcccg tcttgctccg
      181 ggtgacctgt cgatcgtccc tacgtgtagg ttccgtactt gaggaccagc tcgatggtcc
      241 gacagaactg ggcgaaactg acgttgtcgg gtaccgagtg gtcggaatgg tagatatagc
      301 cgccaccctt cttggcgatg gggatcttgg tagcgatctc gcgctcgatg accgcagggt
      361 cgggatcggc catggcgcgg acgtcgatgc cgcccatgag agcgagcacg tcgccgtagt
      421 ctttcttgag ctggaccagg tccatccccg ccttgacttc gaggggctga aggcaatcga
      481 aaccagcctc aatgagcgcc ggcacgtgtt ggttcacgtt gccgcaggag tgcaggatga
      541 ccttgagtcc tctgccgtgg gcgaagtcgc acaggcgctt gtggtagggc atcagaacct
      601 cgcggtaggc gcggggcgag aagaaggggc gatgcttgta gcccatgtcg tcgtagaaga
      661 agacaccatc aaagacgaag ccacgggcca tcatttcctc catggcgttg atgttcagct
      721 cggcgttggc gcggaacatg tcgcgaaccc agtcgggatc ctcgacgaag gccatgagca
      781 ggtcctcggg ccccacgagc atggcggtgc ggtcatagcc aattgcggcg gcataggggc
      841 agaataggcc cgcctcccgg cccgcgttgt ggctcttgag ggcgttgtcc cagtcgacgc
      901 gcgtatcgtt ccacgccatg cggtcgcggt gctcctccca tttggcgcgg gtgttgatgg
      961 cgaacccaag aagctccggc gtggaggtgg agctcttcca gttcttgacc gtcgcgccat
     1021 cggcgttgcg cgcgatcgag tactcgggtg tgtcttcgat tacctcggag gggagctgga
     1081 gactgccgtc gcagccggtg ccaccgaact cgaagccgaa gtactcatgg ggggacttgt
     1141 cctcgggcag tccctcccgg tgccagcggg cgatggtggt tccccagggc ccgtcggtga
     1201 tggcgatgcg atcggcttct ttgtggtcga gggcgaggag gacgcgctca cgcgaggtca
     1261 tggcaccacg tacctctcca aagcggcttg gcccaggtgg gggtggcgga aggctagcac
     1321 ggggctgaag gggcgtcaac ggccgaggcg gcccatgagg cgcggcagtg gggtagcccg
     1381 ggttcgcgaa tccgtgctgg acatgcaaac gcgtgtttgc tatactgaaa ggacagcgac
     1441 gcgagtgagg tagcctgccc atgcggatcg ccgccctcac gcagtacgga ctccccccgg
     1501 cgctcatccc ggtgctcgag caccggttcg gtgccgatct gctggaggtc cagtcgcggg
     1561 ccgtggccga gcatgggctg ctggagggcc ggagcttagt cgtgtgcgcc cccacgggct
     1621 cggggaagac cctcatcggc gagctggcgg ctctgcgagc cgcgacgcat ggtcggcgag
     1681 ggatcatcct cgtgccgaca cgctcactgg ccgaggagaa gtggcacgac ctctcggctg
     1741 cgtacgagcc tttgggtctg cggtgcctgc tctcgacggg cgatcgccgc accaccgacc
     1801 gggacgtgat ccgtggggcg ttcagcctgg cggtgacggt ccccgagaag ctcctggccc
     1861 tgctcgccgc gggcccatcg cgctggctcg agggcgcggc gttggtggtg gacgagctgc
     1921 agcttctggc cgatcccggc cgcggagcgg cactggagga tctgctgacg cgcctgcggg
     1981 cccggggggt gccggagcag gtcgtcggct tgtcggcggt tctgcgcggg gccgacggac
     2041 tggcggagtg gctcggtgcg gaggtggtcc gtggggcaat gcgccccgtt gagctgcgca
     2101 aaggggtact cggcgacggg gtgttctact accgcgagca caacagcggt ctgtggggcg
     2161 aggagcggct cgtccccgag ggctggcagg gtgggctgga tgagctcgac acgggcgacg
     2221 gcaagcggca ggacctgctg acctgtgccc tatcgctggc atcgtcgggc gagagcgtgc
     2281 tgctgttcgt tcgg
//
LOCUS       DAOSPL010000781         2294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46913_length_2294_cov_2.643144, whole genome shotgun sequence.
ACCESSION   DAOSPL010000781 DAOSPL010000000
VERSION     DAOSPL010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46913_length_2294_cov_2.643144"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            369..785
                     /locus_tag="PLQ54_13620"
     CDS             369..785
                     /locus_tag="PLQ54_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94353.1"
                     /translation="MNRLALRLGLYGGFMAFTTSALVMFTADPQFAQRTPAQLLQISL
                     IWFGFAALLGSVIGWGVFAAWSHRPTEAGEAQAIPATGPPSVSANQASRAYAASAQGP
                     IAEEQPEFEEGTAGIFETIPPESTAQVIRGTFQNEQ"
     gene            871..1617
                     /locus_tag="PLQ54_13625"
     CDS             871..1617
                     /locus_tag="PLQ54_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOS94354.1"
                     /translation="MLRQYRQNMSPEEREALILQYAHLVRYVVGRIMVELPRHVDRDD
                     LIGHGTIGLIRAVDKFDPRRGVKFETYAISVIRGAVLEALRQEDWVPRSVRDKSRRVA
                     QAIRQIELELGRPPEDAEIAEALEMTLDEYQALLASIAKISVVSLETGFEADPDSSGS
                     PVDEIADQAPGPDAVYGRAERKQILAQAIDSLPEREKLVIALYYQEGLTLKEIGLVLE
                     VTESRVCQLHTQALLRLRGRLEQLRTSLMG"
     gene            1666..>2294
                     /locus_tag="PLQ54_13630"
     CDS             1666..>2294
                     /locus_tag="PLQ54_13630"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOS94355.1"
                     /translation="MRLNDLGEFGLIDRLAALLPPVPRHVLTGVGDDCAVLDLPGDRA
                     LLFAADACIEGTHFFIETPQIGWKALVANLSDIAAMGGRPVAALAVLAARPDVDVRVA
                     DLIYEDLARASAHYDVPVVGGDTVSTSGPLTLAVSVLGEVPKNQVLRRCGAEPGHVLM
                     VTGWLGCALGGLEVYRARREMWSLAGTIASQKPPMEYQHRLAFVTATLL"
BASE COUNT          381 a          723 c          783 g          407 t
ORIGIN      
        1 gacctgcgca agtacctgga tccagcgcgg tcggccatgg ctgaggtcgt cgccgcgagg
       61 atgactgcct ttggacaggc gggccacgcc aaggacgtgc ccatggtcag cctggacgag
      121 atggccgagc gctatcggtc gggcagtgtc tagcagggat ggccaagctg acacggatgg
      181 acgggggcgc cttcgagcgc ccccgttggc gttttggggg ccgttgatcc aagtaacaac
      241 cccgcaacgg ccgcaggagc gttcagggaa ttgggcctcg ggccacgaat aggcatatgc
      301 agtgagctgg cggcgcccgc cgggccgccg cgcggcgggc catctcggca tggacggagg
      361 ctggtggcgt gaaccggttg gctctcaggc tcgggctgta cggcggcttc atggcgttca
      421 ccaccagtgc gctggtcatg ttcacggccg atccgcagtt cgcgcagcgg acgcccgcac
      481 aactcctgca gatctcactc atatggttcg gtttcgcggc actgcttggg agcgtcatcg
      541 gatggggcgt cttcgccgcg tggagccacc gcccaaccga ggcaggtgag gcccaagcga
      601 tccctgccac agggccgccg tcggtcagcg ccaatcaggc atcccgggcc tatgccgctt
      661 cggcacaggg gcctatcgcc gaggagcaac cagagttcga ggaagggact gcgggcatct
      721 tcgaaacgat cccgcccgag tcgacggccc aggtcatccg gggaaccttc cagaacgaac
      781 agtgatgggg gccacggccg ttggtggagt agagtgagcc cggggctcct ggtttccgtg
      841 tgacagacat ccgtatagga acgtggtttc gtgctaaggc agtaccggca gaacatgtct
      901 cccgaagagc gggaggccct gatcctgcag tatgcccact tggtgcgcta tgtggtcggg
      961 cgcatcatgg tggagttgcc tcgtcacgtg gaccgcgatg acttgatcgg gcacgggacc
     1021 atcggcctga tccgggctgt ggataagttc gaccctcgcc gaggtgtcaa gttcgaaacc
     1081 tatgctatct cggtgatccg cggcgctgtc ctcgaagccc tgcgtcagga ggactgggtt
     1141 ccgcggtccg ttcgcgacaa gtcgcggcgg gtggcccagg ccatccgcca gatcgaactt
     1201 gaactgggcc ggccgcccga ggatgccgag attgccgaag ccctggagat gacgctcgac
     1261 gagtaccagg cgctgttggc cagcatcgcc aaaatcagtg tggtctctct cgagacaggc
     1321 ttcgaggccg acccagactc cagtggatca ccggtagacg agatcgccga ccaggcaccg
     1381 gggcctgacg ccgtctacgg ccgagccgag cgcaaacaga tcctcgcgca ggcgatcgac
     1441 tcattgccag aaagagagaa gctcgtgatt gccttgtact atcaggaggg cttgacactc
     1501 aaggagattg gcctcgtact cgaagtcacc gagtcgcgcg tatgccaact ccatacccag
     1561 gctctgctgc gactccgcgg caggctggag cagctgcgga cctccctgat gggctagcgc
     1621 acgggtgggc ctcgtcatgg cccaatggag ccccaggagt ctcccatgcg cctcaacgat
     1681 ctaggcgagt tcggcctgat cgatcggctg gcagcgctgc tgccgccggt gccccgccac
     1741 gtgctcactg gcgtcggcga tgactgtgcc gtgctcgatc tgcccggcga tcgtgccctg
     1801 ctgttcgcgg ccgatgcctg catcgagggg acgcacttct tcatcgagac cccccagatc
     1861 ggctggaagg cgctcgtcgc gaatctcagt gacatcgcgg ccatgggagg gcggcccgtc
     1921 gccgccctgg ccgttctcgc ggcccgcccc gatgtggacg tgcgggtggc ggatctgatc
     1981 tatgaggacc tggcgcgcgc ttccgcgcac tacgatgttc ccgtcgtggg tggtgatacc
     2041 gtctcaacca gcggaccact caccctcgcc gtcagtgtcc tgggcgaggt gcccaagaat
     2101 caggtgctcc gccgttgtgg ggcggagccg gggcatgtgc tgatggtcac cggttggctc
     2161 gggtgtgctc tcgggggcct cgaggtctac cgggcccggc gcgagatgtg gtcgttggcg
     2221 ggcaccattg cctcccagaa gccgcccatg gagtaccagc accggttggc cttcgtcacg
     2281 gcgacgctgc tcca
//
LOCUS       DAOSPL010000782         2293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46935_length_2293_cov_4.111707, whole genome shotgun sequence.
ACCESSION   DAOSPL010000782 DAOSPL010000000
VERSION     DAOSPL010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46935_length_2293_cov_4.111707"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..1110
                     /locus_tag="PLQ54_13635"
     CDS             46..1110
                     /locus_tag="PLQ54_13635"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOS94356.1"
                     /translation="MQTHGLGTIALAAAALAAVPLPWLPEVQQAPPGAPDAPAAWDLL
                     QGRPHDREHWLARRETIRSWWTEFLGPAPTPPANLACEVIEEDRAGDVLRQLVRLQVE
                     PGCWMEAYLLRPAETTGRLPGVVVLHSTVDYTIRQPAGLEGPESYHIGLHLAQRGMVT
                     LCPGCFIWDYAGATTFGEAVARLRERHPGWRGMGKMLYDAQRAADYLQSLAFVDPSRL
                     GCIGHSLGAKETLFLCALDDRFSAAVSSEGGVGLSFSNWDAEWYLGPSIREPGFAHDT
                     HELLALAAPRPFLLIGGGSADGAQSWPYVAAAMPVYRLMGGTEALGLLVHDAGHSLPP
                     IALERAYEWLGYHLGMATGG"
     gene            complement(1107..2255)
                     /locus_tag="PLQ54_13640"
     CDS             complement(1107..2255)
                     /locus_tag="PLQ54_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS94357.1"
                     /translation="MTSRERVRAALRHTETDRVPIDLGGNQTGITRTAYEGLLRLWGW
                     DEPIEILDAVQQLAQPSERVLDRFGVDTRYIRARGAEDGDGSITEELIRGKPHLSFTD
                     EFGVTWSMPKVGGLYHDITYSPLAAATTVSDVENHPWPDGGDPARFRGVREEALRTLE
                     STDKALVTGISGVVFEFGWYLMGFEHFYGCLSTQPELIEAVLDHTLQYWLDFETGFLD
                     AVGDLVDVVCVGDDLAGQHGMLMSPEVYRRFIKPRQAKLYALIHSKTKAKLWYHSCGG
                     IRPVIPDLIEIGVEVLNPVQVSAAGMDPAELKREYGRDLVFWGGGCDTQHVLPHGTPA
                     EVAAEVKGRLDALAPGGGCVFTPVHNIQPDVPAANIVAMYEAAAEWRG"
BASE COUNT          351 a          799 c          779 g          364 t
ORIGIN      
        1 caaatcgccg gatgagtaac tggaccagcc ttgctgaggc aaccgatgca gacgcatggg
       61 ctcggaacca tagcgttagc cgccgcggca ctggccgcgg tccctcttcc gtggttgccc
      121 gaggtgcagc aggccccgcc gggggcgcct gacgcgcctg ccgcgtggga cctgctccaa
      181 gggcggccgc atgaccgcga gcactggctc gcgcggcgcg agaccatccg gagctggtgg
      241 acggagttcc tcggccctgc gcctacgccg ccagccaacc tggcctgcga ggtgatcgag
      301 gaggaccggg ccggcgacgt gctccgccag ctcgtgcggt tgcaggtgga gccaggctgc
      361 tggatggagg cctacctgct gcgcccagca gagaccacgg gccgcctgcc cggcgtggtc
      421 gtcctgcact cgacggtaga ctacaccatc cggcagccgg ccggactcga agggccggag
      481 agctaccaca tcggactgca cctggcacag cgcggcatgg tgacgctgtg cccagggtgc
      541 ttcatctggg actacgccgg cgcgaccacg ttcggcgagg cggtggcacg cctccgcgag
      601 cgacatcccg gctggcgcgg tatgggcaag atgctctacg acgcccaacg cgccgccgac
      661 tacctgcaga gcctcgcgtt cgttgatccc tcgcggctgg ggtgtatcgg ccactcgctg
      721 ggcgccaaag agaccctgtt cctctgtgcg ctggacgacc ggttctcggc ggccgtgagc
      781 agtgagggcg gcgtcggcct gtcgttttcc aactgggatg ccgagtggta ccttggcccg
      841 tccatccgcg agcccggctt cgcccacgac acgcacgaac tgctggcact ggccgcgccg
      901 aggccattcc tactcatcgg cggcggctcg gccgacggtg ctcagagctg gccttacgtg
      961 gcggcagcga tgccggtgta ccggctgatg ggcggcaccg aggcgctggg gctgctcgtc
     1021 catgacgccg gccactctct gccgcccatc gcgctcgagc gggcctacga gtggctcgga
     1081 taccacctgg gcatggcgac ggggggctag ccccgccact cggccgccgc ctcgtacatg
     1141 gcgacgatgt tcgccgccgg tacgtccggc tggatgttgt gcaccggcgt gaacacgcac
     1201 ccaccacccg gggccagggc atccagccgg cccttgacct cggccgccac ctcagccggc
     1261 gtgccgtggg gtagcacgtg ttgggtgtcg catccgccgc cccagaaaac gaggtcccgg
     1321 ccatactcgc gcttcagctc ggccgggtcc atccccgccg cggacacctg caccgggttg
     1381 agcacctcga cgccgatctc gatcaggtcg ggaatcactg gccggatgcc gccgcacgag
     1441 tggtaccaca gcttggcctt cgtcttactg tggatcagcg cgtacagctt cgcctgccgc
     1501 ggcttgatga accggcggta cacctcgggc gacatgagca ttccgtgctg cccggccaga
     1561 tcgtcaccca cgcacaccac atcgaccagg tcgcccacgg cgtcgaggaa gcccgtctcg
     1621 aagtccagcc agtactgtag cgtgtggtcg aggacggcct cgatgagctc gggctgcgtc
     1681 gacaggcagc catagaagtg ctcgaagccc atcaggtacc agccgaactc gaacacgaca
     1741 ccagagatgc cggtcacgag cgccttgtcg gtcgactcca gcgtgcggag agcctcctca
     1801 cggacgcctc ggaagcgcgc cgggtcgccc ccgtcgggcc acggatggtt ctcgacgtcc
     1861 gacacagtgg tcgccgcagc cagtggcgag taggttatgt cgtggtacag cccacctact
     1921 ttgggcatgg accaagtcac gccgaactcg tccgtgaagg acagatgcgg cttcccgcgg
     1981 atcagctcct cggtgatgct gccatcgccg tcctcggcgc ccctcgcgcg gatgtagcgc
     2041 gtgtcgaccc cgaagcggtc gagaacccgc tcgctgggct gggctagttg ctgcacagca
     2101 tcgaggatct cgatgggctc atcccagccc cacagccgca gcaggccctc gtaggccgtg
     2161 cgggtgatgc ccgtctggtt gcccccgagg tcgatgggta cgcgatcggt ctcggtgtgc
     2221 cgcagagcgg cgcgcacgcg ctcgcgagat gtcaacgatg gtcgcctcca tggtgggtga
     2281 gcggggcagg cgg
//
LOCUS       DAOSPL010000783         2293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46936_length_2293_cov_3.836461, whole genome shotgun sequence.
ACCESSION   DAOSPL010000783 DAOSPL010000000
VERSION     DAOSPL010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46936_length_2293_cov_3.836461"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /gene="lpxD"
                     /locus_tag="PLQ54_13645"
     CDS             <1..887
                     /gene="lpxD"
                     /locus_tag="PLQ54_13645"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOS94358.1"
                     /translation="PCAAPLVRVDDPRLAFLKVMSLFGPAEALPLGVHPTAVVDEGAE
                     LGPDVRIGPFCHVAAGARIGARTRIHAHVTIGPRARVGEDCDLRAGVRVERDCVLGDR
                     VVVFPNAVIGADGFGFAQGETGLEKFPQMGIVRIGSDVEIGANTTIDRATMPEEATII
                     GEGTKIDNLVQIGHNCVIGRQCVVAAQTGISGSVEVGDGCVFGGQSGIRDHVRVGARV
                     RLGGRAGVIQDVPEGSTMSGYPAQDHRAALRQEATLRHLPVMRQQIRELERRLRELTS
                     RAIGSRPDSEESGREPST"
     gene            970..>2293
                     /locus_tag="PLQ54_13650"
     CDS             970..>2293
                     /locus_tag="PLQ54_13650"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOS94359.1"
                     /translation="MSDYRIERDTLGDVEVPAQALYGAQTQRAVRNFPISGLRLPPEF
                     IRAQAVTKWAAARANMDGGRLDPDIGGALVSAAEEILAGQHHEHFVVDVFQAGAGTSQ
                     NMNMNEVLAHRASQLLAARGLTEVRVSPNDHANMGQSTNDTIHVAIHVAAYCEIHRRL
                     IPAAEELSRALRHKAVEFDPIVKAGRTHFQDAVPVRLGQEFGAYATMVDKGVHRVSHA
                     AEALRELAMGGSAVGTGLNTFPGYRRLAVGYICEITGYEFVPCEDRFEAMQCFDAVVE
                     TVGALRVLVTSLRKIAADLILLSSGPRTGLSEIRLKPVQPGSSIMPGKVNPVLPEMLS
                     MVCFHALGNDMAVVAAAQAGQLELNVMMPIIAYNLLQEIAILSGGMSAFTEHCVWHIE
                     ADEATCRRNAERSPALATALNPAIGYFRAAELAKEALDRDLLIRDLVVE"
BASE COUNT          369 a          787 c          748 g          389 t
ORIGIN      
        1 cgccgtgcgc ggcgcccctg gtccgggttg acgatccccg cctggcgttc ctcaaggtca
       61 tgagcttgtt cggcccggcc gaggccctcc cgctcggagt ccatcccacg gccgtcgtcg
      121 atgagggggc ggagctcggt cccgatgtcc ggatcggtcc cttctgccat gtggccgccg
      181 gcgcccgaat cggcgcgcgc acgaggatcc acgcccacgt taccatcggg ccccgagccc
      241 gtgtcggtga ggactgcgat ctccgggccg gcgtccgcgt cgagcgcgac tgcgttctgg
      301 gcgatcgggt cgtcgtcttt cccaatgcgg ttatcggggc cgatgggttc ggcttcgccc
      361 aaggagagac cgggctcgag aagttccccc agatgggtat cgttcgaatc ggcagcgatg
      421 tcgagatcgg ggccaacacc accatcgacc gcgccaccat gcccgaagaa gccacgatca
      481 ttggcgaggg cacgaaaatc gataatctcg ttcagatcgg ccacaactgc gtcatcggtc
      541 ggcagtgcgt cgtcgccgcc cagacgggaa tctcgggcag cgtcgaagtg ggcgatggtt
      601 gcgtcttcgg cggccagtcg ggcatccgtg accacgttcg cgtgggcgct cgggtgcgcc
      661 tggggggacg tgccggcgtg atccaggatg tcccggaggg ctcgaccatg tcgggctacc
      721 cggcccagga ccaccgtgcc gccctgcgcc aggaagcaac actccggcat ctcccggtca
      781 tgcggcagca gatccgtgag ctcgaacggc gcctgcggga gctgaccagt cgggccatcg
      841 gttcgagacc agattctgag gagtcgggga gggaaccctc tacctaggtg tccgcgttgg
      901 gtcagaagcc cgcagccccg tcgtggcatg gggtgggccg cagcgctcca atgaacggat
      961 gcacgatcga tgagtgacta tcggatcgag cgcgataccc tgggcgatgt ggaagtcccc
     1021 gcccaggcac tctacggcgc ccagacccaa cgtgccgtac gcaacttccc catcagtgga
     1081 ctgcgcctcc ctcccgagtt cattcgggcc caggccgtca ccaagtgggc cgctgcccgt
     1141 gccaacatgg acggtggccg gctggatccc gacatcggcg gcgctctcgt ctccgctgcc
     1201 gaagagatcc tcgccggtca gcaccatgag catttcgtcg tcgacgtgtt ccaggcgggt
     1261 gcgggcacgt cgcagaacat gaacatgaac gaggtgctcg cccaccgtgc gtcccagttg
     1321 ctcgcggccc gtggcctgac cgaggtgcgg gtcagtccca acgatcacgc gaacatgggg
     1381 cagtccacca atgacacgat ccatgtcgcg atccatgtcg ctgcctactg cgagatccac
     1441 cgccgcctga tcccggccgc cgaagagctg agccgggcgt tgcggcacaa ggcggtcgag
     1501 tttgatccca tcgtcaaagc gggccggacg cacttccagg atgccgtccc tgtgcggctc
     1561 ggccaggagt tcggcgccta cgccaccatg gtggacaagg gggtccaccg cgtgtcccat
     1621 gccgccgaag ccctgcgcga gctggccatg ggaggtagcg ctgtcggaac gggcctcaac
     1681 accttccccg ggtaccgccg tctggccgtc ggctacatct gcgagataac cgggtacgag
     1741 ttcgtgccct gcgaggatcg ctttgaggcc atgcagtgct tcgatgccgt cgtcgaaacg
     1801 gttggggcgc tgcgtgtcct ggttaccagc ctccgcaaga tcgccgccga cctgatcctg
     1861 ctctcctccg gcccgcgcac gggtctgtcg gagatacggc tcaagcccgt ccagcccggc
     1921 tcctcgatca tgcccggtaa ggtgaaccct gttctgccag aaatgctcag catggtgtgt
     1981 ttccacgccc tgggcaacga catggccgtc gtggccgccg cccaggcggg ccagctcgag
     2041 ctcaacgtga tgatgcccat catcgcctac aacctgctgc aggagatcgc catcctgtcc
     2101 ggcggtatgt ccgccttcac cgaacactgc gtgtggcaca tcgaggccga cgaggcgacg
     2161 tgccggcgga acgccgagcg gagccccgcc ctggcgaccg ccctgaaccc cgccattggc
     2221 tacttccgcg ccgcggagct tgccaaagag gccctcgacc gcgatctcct gatccgagac
     2281 ctggtggtcg agc
//
LOCUS       DAOSPL010000784         2293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46942_length_2293_cov_3.061662, whole genome shotgun sequence.
ACCESSION   DAOSPL010000784 DAOSPL010000000
VERSION     DAOSPL010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46942_length_2293_cov_3.061662"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PLQ54_13655"
     CDS             <1..911
                     /locus_tag="PLQ54_13655"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOS94360.1"
                     /translation="GQVTEQPDSPPYRGQIRRVEGRNTKGGKRSGLPKIHEIELEHQG
                     GLRIEAHSHKPPTGDGTRTATGVCVIEPGQFPYCPICLREDIPAATMEHIPPKAFGGA
                     AMANTCADCNNGLGSRTEAALQDWFDGAIRARFTSGSSPQPFGHDRILMLTTEAGECV
                     MLAEKSSPEGASPFDRLKDPNVEIHYTFPRPAEYKTGLLKSAYLAACLHFGGVAHAPS
                     MMAARDELLRARDAKSRRGVVIGPIAEKLTAHRTGLAANGPTLALLRSDHEGESTYLI
                     SLAGTILVEWPFVDLDPMLSPRMGAA"
     gene            complement(931..1365)
                     /locus_tag="PLQ54_13660"
     CDS             complement(931..1365)
                     /locus_tag="PLQ54_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94361.1"
                     /translation="MLPIVLSRGTCELVIQNIGASVAKNILVTFDPPIRESMGVTAGY
                     LARRYEQPIPTMGPGRRLTNVYGHRRGDGTAAFDEDVPLDIAVTVDYEDSHGRQYRDS
                     YALTSQTLMNETTTSPSNTDEKGIQRRLVQALEAIARGVANR"
     gene            complement(1587..1868)
                     /locus_tag="PLQ54_13665"
     CDS             complement(1587..1868)
                     /locus_tag="PLQ54_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94362.1"
                     /translation="MGFYKPGICIDSFEPEPDRLVRVRRYDVEETYDLGEIVAIDDSE
                     LMRFARVVRYEGDAFTGCICLEELHGEEAEQAAMDLELSYEEWCDPLEG"
     gene            complement(1946..2269)
                     /locus_tag="PLQ54_13670"
                     /pseudo
     CDS             complement(1946..2269)
                     /locus_tag="PLQ54_13670"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          417 a          755 c          720 g          401 t
ORIGIN      
        1 atggacaggt gaccgaacag cctgactcgc cgccgtatcg cggccagatc cgccgagtcg
       61 agggtcggaa cacgaaggga ggcaagcgga gcgggctgcc gaagatccat gagatcgaac
      121 tcgagcatca gggtggtcta cggatcgagg cgcatagcca caagcctccg accggcgacg
      181 gcacgcgcac cgcaaccggc gtgtgcgtca tcgaacctgg tcagtttccg tactgcccca
      241 tctgccttcg cgaagacata cctgccgcca ctatggagca catcccaccg aaggcgtttg
      301 gcggtgcggc gatggcgaat acgtgcgcgg attgcaacaa cgggctgggg tcccggacag
      361 aagcagccct ccaagactgg ttcgacggcg cgatccgagc acgcttcacg tcgggcagca
      421 gtccgcagcc cttcggccat gaccggattc tgatgctcac caccgaggcg ggcgagtgcg
      481 tgatgctcgc ggagaagtcc tccccggagg gcgcatcacc gttcgacaga ctcaaggatc
      541 cgaacgtcga gatccactac acgttccctc gcccggccga gtacaagaca ggtttgctca
      601 agagcgcata ccttgcagcg tgtctccact tcggcggcgt cgcgcacgcg ccgagcatga
      661 tggccgcccg cgacgaactc ctccgggcgc gtgacgcgaa gtcgcgtcgc ggggttgtaa
      721 tcggtccgat cgccgagaag ttgacggcgc accgaacagg tctcgcggcc aacggcccca
      781 cgttggcact cctacgctcg gaccacgaag gcgaaagcac ctacttgatc tcactcgccg
      841 gcacgatcct cgtcgagtgg ccgttcgttg accttgaccc gatgcttagt ccgcggatgg
      901 gggccgcgta gcggcgaggg accgctccgc ctatcggttc gcgacgccgc gggcaatcgc
      961 ctccaacgcc tgcacgagcc ggcgctggat ccccttctcg tcagtgttgg atggagaggt
     1021 cgtggtctcg ttcatcaggg tctgggatgt caaagcgtaa gaatcccgat actggcgacc
     1081 atgactgtcc tcgtagtcga cagtcacagc gatgtccaat gggacatcct cgtcgaaggc
     1141 cgcagtaccg tcgcctcggc ggtggccgta gacgttcgtc agacggcgac ccgggcccat
     1201 cgtcggtatt ggctgctcgt atcgacgcgc caagtatccg gccgtcacgc ccatgctctc
     1261 tcgaatcgga ggatcaaacg tgacaaggat gttctttgcc acagatgcac cgatgttctg
     1321 aatcaccagc tcgcaggtgc cgcgcgacag gactatcggc agcaactcaa ctccgacaac
     1381 aggccgtgtt cggtctctgc tgtccactcg caaagcccgg agtgttgcga tagccacgac
     1441 gctggtgaca agggcgcaca caccggtaac cgcagtccag aaagcggtca actggtccgg
     1501 cgtgtacgag tttaggggca cacccccatg ttggcggatc gaagttctcc cacatgccgg
     1561 ccacgccggt ccaccgcgcg aacgccctac ccttccagcg ggtcgcacca ctcttcgtag
     1621 gagagctcca ggtccatggc ggcctgttcg gcttcctcgc cgtgcaactc ctccaggcag
     1681 atgcagcccg tgaaggcatc gccctcgtac ctcacaaccc gagcgaatcg catgagctcg
     1741 ctgtcgtcga tggcgacgat ctctccgagg tcgtaggtct cctcgacgtc gtaccggcgg
     1801 acccggacga ggcggtcggg ttcgggctcg aaggagtcga tgcagatgcc tggtttgtag
     1861 aagcccacag tcctccccct tccgtgtaga cggccgccac ggcggcgcgt gaggtgtcgt
     1921 ccctgtccgg ccagaggtgg ccgtaggtgt cgagagttgt cttggccgat gcatgccgca
     1981 gtcgcgtctg gacgaccttc acgtcgagac cggaggcgat cagcagcgaa gcgaagtagt
     2041 ggcgcaggtc atggaatcgg aagccctcag gcagccccac ctgctcacga gccgcgcgga
     2101 tcgcgcgctc gatggtccag gggccggctg ggttgcccca ttcgtcgtct gcgagggtcc
     2161 gtccgttgcc caccctgacg gcctcggcca gcaggtgagc catctgcttg gggatcggga
     2221 tcggcgtgcg gctcttctca gacttcaacg gctcgtcccc ccactgcacc gatggcgtta
     2281 caagcccggt cgc
//
LOCUS       DAOSPL010000785         2293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46946_length_2293_cov_2.803843, whole genome shotgun sequence.
ACCESSION   DAOSPL010000785 DAOSPL010000000
VERSION     DAOSPL010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46946_length_2293_cov_2.803843"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1673)
                     /locus_tag="PLQ54_13675"
     CDS             complement(<1..1673)
                     /locus_tag="PLQ54_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94363.1"
                     /translation="MARPCDPPAERLCRLAMLALLPLFLVSSARGEVTAEYGEIRDGR
                     WVFPDIAGPSRSDLAQHATIRVVAGALEPAGRGVDGLNDGTVPCVGGIQEAGVSLTSA
                     NVDGGKLLMDLGSPQAIHQVNSYSFHRYEPDQGARGPQVYSLYGSAAPEEPDANDPAH
                     SAAWTKVADVDSRPNAAGEGWGGRVHGVQIAAPNGDLGTFRWLLWDIRPTRSPLAYAP
                     SYTHTMYAEFDVHSTESLARSQPADRWTPFPDVKRVVLAFKTHFDIGYTDLAREVLER
                     YRTTMIDDALKVSDELGDLPGGQRFVWTVPGWPMEYILAHQPPGGAPRRARIETALRA
                     GTFAVHALPFTLHTESLDLEDLCRGLTHSSDLCRAMGLPLPRDAKMTDVPCHTWVVPT
                     LLAHAGVEFLHIGCNPASQSPEVPPLFWWEGPDGSRVLTQYSTQYSDGLMPPEGWPHA
                     AWLAIIHTGDNQGPPPAFEVRQWVEEAPQLFPGAEVTIGRLSDFADAMRELGGEIPIV
                     RGDMPDTWIYGPMSDPDGVASVRNARRCLAVAEALHTQLRTWGATRRDI"
BASE COUNT          354 a          745 c          831 g          363 t
ORIGIN      
        1 ttgatgtcgc gccgcgtcgc tccccaggtc cggagttggg tgtgcagggc ctccgcaact
       61 gccaggcagc ggcgtgcgtt ccgcaccgag gccacgccgt cagggtcgga catggggccg
      121 tagatccagg tgtcgggcat gtcgccccga acaatcggga tctcgccgcc aagctcgcgc
      181 atggcgtcgg cgaagtccga cagacgcccg atggtcacct cggcgccggg gaaaagctgg
      241 ggtgcttctt cgacccactg tctcacttcg aaagcgggag gcggcccttg gttgtcgccg
      301 gtgtggatga tggccagcca ggcggcgtgc ggccacccct ccggcggcat caggccgtcg
      361 gaatactgcg tcgagtactg ggtcagtacc cgcgagccgt cggggccctc ccaccagaac
      421 agcgggggca cttcgggaga ctgggacgcc gggttgcacc ctatgtgcag gaactcgacc
      481 cccgcatggg ccagcagcgt gggcaccacc cacgtgtggc aggggacgtc ggtcatcttg
      541 gcatcgcgcg ggagcggcag ccccatggcg cggcacaagt ccgagctgtg cgtgagcccg
      601 cgacagaggt cctccaggtc caacgactcg gtatgaagcg tgaatggcag ggcatggacg
      661 gcgaaggttc ccgcgcgcag ggcggtctcg atccgggcac gccgcggcgc gccgcccggc
      721 ggctgatggg cgaggatgta ctccatgggc cagccgggca ccgtccagac gaagcgctgt
      781 cctccaggga ggtcaccaag ctcgtcgctg accttgagtg cgtcgtcgat catcgtcgtg
      841 cggtagcgct cgagcacctc tcgcgcgaga tcggtgtagc cgatatcgaa gtgggtcttg
      901 aaggcgagaa cgacgcgctt cacgtcgggg aacggtgtcc accgatcggc cggctgcgag
      961 cgggcgaggc tctctgtcga gtggacgtcg aactcggcgt acatggtgtg ggtgtacgag
     1021 ggggcgtaag ccagcggtga ccgtgtgggc cggatatccc agagcagcca gcggaaggtg
     1081 ccgagatcgc cgttgggcgc ggcgatctgc acgccatgga cgcgcccgcc ccagccctcg
     1141 ccggcggcgt tggggcggct atcgacgtcg gcaaccttcg tccaggcagc ggagtgagcg
     1201 ggatcgttcg cgtccggctc ctcgggcgcg gcgctcccgt agaggctgta cacctgcggc
     1261 ccacgcgcgc cctggtcggg ctcgtagcgg tggaacgagt aggagttgac ctgatggatc
     1321 gcctgaggcg agcccaggtc catgagcagc ttcccgccat cgacgttggc gctcgtgagc
     1381 gacaccccgg cctcctggat tccgcccacg cagggcacgg tgccgtcgtt caggccatcg
     1441 acgcctcggc ccgccggctc gagagcgccg gcgaccaccc gtatggtggc atgctgagcc
     1501 agatcggacc tcgatgggcc ggcgatgtcc gggaacaccc atcggccgtc acggatctcc
     1561 ccgtactcag cggtgacctc gcctcgggca ctcgagacga ggaagagcgg aagcagtgca
     1621 agcatcgcga gcctgcacag gcgctccgcg ggtggatcgc atggtcgcgc catgggccgg
     1681 attccctccg tccttccact ggtagacggc cccgcattgc gacgcggacg cgtccgatcc
     1741 cttccatgcc ggaggagggc gagagctcag agcgggtgtg ggtgaagcgc gtcagggggt
     1801 ggggagtcac gaggaggccc atatggccgt gctggcctcg agggcgtcgg gccgcgcagt
     1861 gatcaggtcc cgcacgggat cgcagcatcg ccatggtccg cgagccgcgg gcggtccacc
     1921 acgctgcgag caccgttcgc gcggcggtca acggccgggt ccctcgctcc gccggggcgg
     1981 ccgcgcctca gagagcctgt gaagcaccac ccactgggcg atgggccgga gcttaaccca
     2041 acgcctgctc catgacccct gcgcttcagt cccacacgtc ggcgatctgg aaccagtcct
     2101 ggtggagcat ggtgggattg ctcatgagct gctgcttgct gatccacttg gcgtggctgt
     2161 cgacgaacac gttgttgcag ccgccatgtg ccggtcggtg agcatgtcga agatctcctg
     2221 gttggtggtc gatccgggct caaggtacgg cgcgccggcg gggtagccat ccacggtggg
     2281 cagctcaatg gtc
//
LOCUS       DAOSPL010000786         2291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_46997_length_2291_cov_2.684258, whole genome shotgun sequence.
ACCESSION   DAOSPL010000786 DAOSPL010000000
VERSION     DAOSPL010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46997_length_2291_cov_2.684258"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..1913)
                     /locus_tag="PLQ54_13680"
     CDS             complement(141..1913)
                     /locus_tag="PLQ54_13680"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOS94364.1"
                     /translation="MMRLTLSLALTAGTLVPTAVLGQESPTGRWDVVAAGAVGDGATD
                     CTAIFQALLDGAGQAGGGVVEVPAGRFRLDGTLTVPANVTLQGLFRWAPSMNSVAAAT
                     GSVLCAYAGRGAPEGPAFIRLAGDNAGVAGVVIVYPEWSQNDVPPVAYPPCVASEDTL
                     NVSIQDCMLLNPYEGIRLVRAHRHLVRNITGYPSFRGIFVDECYDIGHVENVHLWPFG
                     TAYDPDNAYSKWVNTQGVAYEFARTDWEYVSNTFCFGYGVGYRFSASEHGSANGNFLG
                     LGADSCERAVLVEQAQPPGLLITNGEFVGRWGSQQAVCVEVGPEVEGVVSLSNCSFWG
                     PIDRCVWMRSANGQFTASACHFIHWDNRNVGSPALQIDAGRALVQGCSFHQDNLDVRV
                     GPAVESAILTANQAPGGFRVENQAGDRTVMGLNQADPIVWTDEARGHYRVEIGANGDG
                     RYISGFGGRERLDAPFRWSTPSSHLILPVPTGKACTVTLEGEIPIHAVAADSGLYLEG
                     RLLAALVAGNRLVAELPPMPDGQVRLELRCQGWCPQEVIPGSTDPRTLGILLRTVTVR
                     ATDAGDAVFGANTGQWLEAAPPAE"
BASE COUNT          348 a          817 c          765 g          361 t
ORIGIN      
        1 ccgccaatgt ccccatgtgg cccctccccc aggcccggac gtgccgtctc ggtactcctg
       61 cggggtcacc tcggacctca ctgagcgccg atggggcaga gccccccagg tcgccgctcg
      121 gtccgagccc ggcggctgtg tcactcggcc ggcggcgctg cctcgagcca ttgacccgtg
      181 ttggcgccaa acacggcgtc gcccgcatcc gtggcccgga ccgtcacggt gcgcagcaag
      241 atgcccagtg tgcgaggatc ggtggacccg ggtatgacct cttgcgggca ccatccctgg
      301 cagcgcagct ccagacgtac ttggccgtcc ggcatgggcg gcagttcggc caccaggcgg
      361 ttccccgcca cgagcgcggc gagcagtcta ccttcgagat acaggccaga gtccgcggcc
      421 accgcgtgga tggggatctc accctcgagc gtgacggtgc aggccttgcc ggtcggcact
      481 ggcagaatca ggtggctgct cggagtgctc cagcggaaag gggcatcgag ccgttcacgg
      541 ccgccaaagc ccgagatgta gcgcccatca ccgttggccc cgatttcgac gcggtagtgc
      601 cctctcgcct catcggtcca gacgatgggg tcggcctggt tgaggcccat gacggtccgg
      661 tccccggcct ggttctcgac tcggaagccc cctggggcct ggttggcggt gaggatcgcg
      721 gactccaccg ccggacctac ccggacgtcc aggttgtcct ggtggaacga acagccctgc
      781 acgagagctc tgcctgcgtc gatctgcaac gcgggggagc ccacgttccg gttgtcccaa
      841 tgaatgaaat ggcatgcgct ggccgtgaac tggccgttgg cgctccgcat ccacacgcag
      901 cggtcgatgg gtccccagaa ggagcagttg ctgaggctga ccacgccctc gacctcgggg
      961 ccaacctcca cgcacacggc ttgttgtgag ccccagcggc ccacgaactc gccgttggtg
     1021 atcagcaggc cgggcggttg ggcctgttcg acgagcacgg cccgctcgca cgagtcggcg
     1081 cccaggccca ggaagttgcc attggcgctg ccgtgttcgg aggccgagaa ccggtagccc
     1141 acgccgtacc cgaagcagaa cgtattactc acgtactccc agtcggtgcg cgcaaactcg
     1201 taggccacgc cctgggtgtt gacccacttg ctgtaggcgt tgtcggggtc gtacgcggtc
     1261 ccgaagggcc acaggtggac gttctcgacg tggccgatgt cgtagcactc gtcgacgaag
     1321 atgccccgga agctcgggta gcccgtgatg ttccgcacca ggtggcgatg ggctctcacc
     1381 aggcgaatcc cctcataggg attgagcaac atgcagtcct ggatgctcac attgagggtg
     1441 tcctcggacg ccacgcaggg cgggtaggcg acgggcggca cgtcgttctg actccactcg
     1501 gggtagacga tgaccacgcc ggccaccccg gcgttgtccc ccgcgagccg gatgaacgcc
     1561 gggccctcgg gggctccccg gccggcgtat gcgcacagga ccgaccctgt ggcggccgcc
     1621 accgagttca tcgacggcgc ccagcggaac aggccctgca gcgtcacgtt cgccggcacc
     1681 gtgagggtcc cgtccaggcg gaagcggccc gccggcacct cgaccacgcc gcctcccgcc
     1741 tggccggctc catcgagcag ggcctggaag atggccgtgc agtccgtggc gccgtcgccg
     1801 acagcgccgg cggcgacgac gtcccaacgc ccggtcgggc tctcctgacc gagcactgcc
     1861 gtggggacca gtgtcccggc ggtcagcgcg agtgacaggg tgagtctcat cattcgcccc
     1921 cctcgggtcg gtgcctcagg gccgaactga tatgctcata tgctcatatg gccagttgcc
     1981 ttcgtgcagc ggcaccataa cattaccatc cggatggtcg cgcgaccacg gtgaccgacc
     2041 acgcggggcg gcggccctgc ggccccgccc tttgacagcc gccgaccgcg ggggtttact
     2101 gtcccggtgt tcgtcaccgg agaggatgga ccgcggatga gatccttgcc catcgtgctc
     2161 gtcaccttgg ccctgttggc cgccctgggc tgcaggaagc cgccggccga gcctgacacg
     2221 acgcccccgc cgccttcggg cgccatcgcg acacccccgg aagcaccggc accgcccccc
     2281 gaggctcccg g
//
LOCUS       DAOSPL010000787         2289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47042_length_2289_cov_3.623545, whole genome shotgun sequence.
ACCESSION   DAOSPL010000787 DAOSPL010000000
VERSION     DAOSPL010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47042_length_2289_cov_3.623545"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..1587
                     /locus_tag="PLQ54_13685"
     CDS             301..1587
                     /locus_tag="PLQ54_13685"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOS94365.1"
                     /translation="MDEWPLGLLLVVVAGLLGLQALFCASEIAFFATGPVKARRFAQR
                     GTRGGRALVRLLENRSALLTTILVVITGVLSVSEALLTHRLLVLMPEYGHWVALFGLT
                     AVVVLFVEVTPAVIASKDPERVSLALAPFVLVVHRVLYWVAAPLGLVARCILWWFGVR
                     QQRPTVTADEIISMIDESEVGDAEKHMLRSAITFADRTVSEVMVPRGEVVCVEESDCV
                     ATAAARMVAEKHSRLPVYREHRDHVVGIVYSKDLLPVLKEGQADRAVGDFARPAYHVP
                     ETMQVADLLAEFQAQRRLMAIAVDEFGATAGVVTLEDVLEEVVGEILDEYDEAEPPDA
                     VEEAEGVYLVSGQAHIHELNRDYGLDLPEDGFETVAGLVNSLLGRLPEGGERVKLGEG
                     HLEIEVLEVVARRVARARLIRRVMGSTEGSEVTPTA"
     gene            1741..>2289
                     /locus_tag="PLQ54_13690"
     CDS             1741..>2289
                     /locus_tag="PLQ54_13690"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HOS94366.1"
                     /translation="MDWSHPAVLVALAAGLAALCMMAFLSLSELALVSVSRPRVRKRA
                     EEHDRRAELLMVLFADEDASYLSGIMIAINVLVIVVANTTALVAIRTGHGSHGPWIAA
                     VQILVTLLVGEIIPKTLATHHAERYALLVAPPVHRLVHSLVFRWGLGVVNAVSRPVRR
                     LFGVTELRGRVLVSSEEIEALAD"
BASE COUNT          312 a          677 c          828 g          472 t
ORIGIN      
        1 accgagagtt gcacagccga tctcgtggcg gacgcgcgac gcctcgagcc gcgtctttgt
       61 gggcgtggtc tgcgcgtagt cgcccagcac cccagccacc acctcgtcca gaacgcttgc
      121 gcgctatcct attataccag ttctgagccg ttttgtaaag tacttcatgc gagcaaatgt
      181 gtgcacacgt tcatcggggt gggggcggcg gatgcagctc gctcccgcgt cgtgacaggc
      241 cggaagtgga ggtatacact agatctgggg ggaagaatcc ccccggggga acgagtttct
      301 ttggacgagt ggccactcgg cttgctcctg gtggttgttg cgggactgct ggggctgcag
      361 gccctgttct gtgcttctga gatcgcgttc ttcgccactg gcccggtcaa agcccggcgc
      421 ttcgctcagc ggggcactcg cggcggacgc gcgctggtgc gtctgcttga gaaccgttct
      481 gctctgctca cgaccatcct cgtggttatc accggtgtgt tgagcgtctc cgaggccctg
      541 ttgacccacc gtctgttggt gctcatgccg gagtatggcc actgggttgc cttgttcggc
      601 ctcacggcgg tcgtggtgct gtttgtggaa gttacgccgg ccgtgatcgc ctccaaggat
      661 ccggagcggg tttcgctggc cctggccccg ttcgtgctcg tggtgcatcg tgtgttgtat
      721 tgggtggcgg ctccgctggg tctggtcgcc cggtgcatct tgtggtggtt tggtgtgagg
      781 cagcagcgtc cgacggtgac cgctgacgag attatctcga tgattgatga gtccgaggtc
      841 ggcgacgctg agaagcacat gctgcggagc gccatcacgt tcgccgaccg cacggtaagc
      901 gaggtcatgg tccctcgcgg cgaggtggtc tgtgtcgagg agagcgactg cgtggccacg
      961 gccgcggcgc ggatggtggc cgagaagcac tcgcgcctgc cggtctaccg ggagcatcgg
     1021 gaccacgtgg tcggcattgt gtactcgaag gacctgctgc cggtgctgaa ggaggggcag
     1081 gcggaccgcg cggtgggtga tttcgcacgc ccggcctatc atgtgcccga gaccatgcag
     1141 gtggctgacc ttttggccga gttccaggcc cagcgacggc tcatggccat cgccgtcgac
     1201 gagttcgggg cgaccgcggg tgtggtgact ctggaggatg tgttggagga agtggtcggg
     1261 gagatcctcg acgagtatga cgaggcggag ccccccgacg cggtcgagga agccgagggc
     1321 gtctatctgg tgagcggcca ggctcacatt cacgagctga accgggacta tggcctggat
     1381 ctgcccgagg acggtttcga gacggttgcg gggctggtga actcgctgtt gggccggctt
     1441 ccggaagggg gggagcgggt caagctaggg gaaggtcacc tcgagatcga ggtgctcgag
     1501 gtggtggctc ggcgcgtcgc ccgggctcgt ctgatccgcc gagtcatggg ttcgaccgag
     1561 ggctcggagg ttaccccaac cgcctgacgc atggacgcca ctactgacga acctaacagt
     1621 ccgttcgccc gtcgcccggc gcggcatggc cgctcggtgg ctgtgttgct gggggctcct
     1681 ctcgccgccc acgccctgtg ggcgatgggg gatggctctg ccccttcgac gcgggctccg
     1741 ttggattggt cgcaccccgc cgtcctggtg gcgctggccg cggggctggc ggccctgtgc
     1801 atgatggcct tcttgtcgct ttccgagttg gctctggtca gcgtcagtcg cccgcgcgtc
     1861 cgcaagcggg ccgaggagca cgaccgccgg gccgagttgc tgatggtgct gtttgcggac
     1921 gaggatgcca gctatctctc gggcatcatg atcgccatca acgtcctggt catcgtggtg
     1981 gccaacacca cggccctggt ggcgatccgg acggggcatg gatcccacgg cccatggatc
     2041 gcggcggtcc agatcctggt cacgctgctc gtgggtgaga tcattcccaa gaccctggcg
     2101 acccatcatg cggaacgcta cgcgctcctg gtggctccgc cggttcaccg gctcgtccac
     2161 agtctcgtgt tccgctgggg tctgggcgtg gtgaatgccg tgtctcgccc ggtgcgccgc
     2221 ctgttcggtg tgaccgagtt gcgcgggcgg gtgctggtca gtagcgagga gattgaggcc
     2281 ttggccgat
//
LOCUS       DAOSPL010000788         2289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47048_length_2289_cov_2.654432, whole genome shotgun sequence.
ACCESSION   DAOSPL010000788 DAOSPL010000000
VERSION     DAOSPL010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47048_length_2289_cov_2.654432"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PLQ54_13695"
     CDS             complement(<1..250)
                     /locus_tag="PLQ54_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94367.1"
                     /translation="MLTLALLGLLNTSAAVDIANPGFEQGAAGWTIETGRAGDDTGGP
                     NRVGFASKGAAEGHTCAMLRYQSSWLWLTTSSLAASVSG"
     gene            498..>2289
                     /locus_tag="PLQ54_13700"
     CDS             498..>2289
                     /locus_tag="PLQ54_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94368.1"
                     /translation="MHQPPVESLARILVDHGWAPVAAIAAGLLALGLASPAHGQPEAL
                     EIAARNFQRGDASVLASYGEPLGIGGIWAEYDFEIATGGSFVLAARYAAGESRPVRLS
                     IDGVLLDDRAMAGNTGSFASAAGRWEDQAVVRLQAGRHTLRVERDPCLPHIAAFRLTP
                     TTAPARAPVLMRRQAALTALLADLEGALKAIPGPAAAELLQKVADLAAAVGQVDLAVA
                     AADATKAVDDLAARASALRVPVAGLRTEALAGSRDRIVAWAGDPGVKVFRDTLLPETA
                     RTDGVELEAVRNSYEAAVVCVSSFDYAGPLRATASPLVSADGGASLTEITTRFVGCVP
                     SPKNTPGWPDVLRAAPASFPDPLLLDPEVALEPRQTQSVWVTVHVPAHAAPGEYSGSI
                     TLSTADAPATVVPLRLRVYRPALPNVPSFQMGSWGGDTFLANEAEGKPRDDKSYDNAY
                     FAFLKDECLRDRFEHRARVFSDVPLWSVFGTCGVSWRDGQYAFDYTLFDRFISTVEEA
                     MHGSFRVICVGIAAGTSEDKTQLLSGDIAVTNADGSPNAERSFANVPTDAPRYREFLG
                     AFMHDMQEHLRAKGWLERVYFKVLDEVSGAT"
BASE COUNT          333 a          780 c          816 g          360 t
ORIGIN      
        1 tcccactcac agaggcggcc agcgacgatg tcgtgagcca cagccacgag ctctggtaac
       61 ggagcatcgc gcaggtgtgg ccctcggcag cgcccttgga ggcgaaccca acgcggttcg
      121 gcccgccggt gtcgtccccg gcgcgaccag tctcgatcgt ccagccggcc gccccctgct
      181 cgaagcccgg gttggcgatg tcaaccgccg ccgaggtgtt cagcagcccc agcaatgcga
      241 gtgtcagcat gatcgtgcct cctgggtggt ccggcaaagc cgacccgagg gcgattgctg
      301 cctactcggg ccacgggtcc gtaggtgggc gcgcggtcca caacaccggc cgggggttct
      361 ccgccgagga gtacccttcc tggggcgcct gccaacgccc tgcgcgtgcc ggagggggca
      421 tggctacggg gggggaacca cgcgctgaaa gtgtgcccaa ctccgcggcg gcactgcgcc
      481 gcgaaggagc tgcctgcatg catcagcccc cagtggagtc tctggcacgg attctggttg
      541 atcacggttg ggcgcctgtc gctgccatcg cggcggggct gctggcgctc gggctggcga
      601 gtccggcgca cggccagccc gaggccctgg agattgccgc ccggaacttc cagcggggtg
      661 acgcctcggt gcttgccagt tacggtgagc cgctgggcat cggggggatc tgggcggagt
      721 acgacttcga gatcgccacc ggcggtagct tcgtgctcgc ggcccggtac gcggcgggtg
      781 agtcgcgccc cgtcaggctc tcgatagatg gtgtcctcct tgacgaccgg gccatggcag
      841 ggaacaccgg tagcttcgcg tccgcggccg gccggtggga ggaccaggcg gtcgtgcgcc
      901 tgcaggcggg aaggcatacg ctgcgggtcg agcgcgaccc gtgcctgcca cacatcgccg
      961 ccttccggct gaccccaacc accgcacccg cccgcgcccc ggtgctgatg cgccgacagg
     1021 ctgcgctgac cgcgctgctg gccgatctcg agggggcgct gaaggcgatc ccggggccgg
     1081 ccgcggccga gctgcttcag aaggtagccg acctggccgc cgccgtcggg caagtggact
     1141 tggcggttgc ggcagccgat gccacgaagg ccgtcgatga cctggcggcg cgagcgtccg
     1201 ccctgcgggt gcctgtggct gggttgcgca cggaggccct cgccgggagc agggacagga
     1261 tcgtcgcctg ggccggtgat cccggtgtga aggtgttccg cgatacgctg ctcccagaga
     1321 ccgccaggac agacggagtc gagctggagg cggttcggaa cagctacgag gccgcggtcg
     1381 tgtgcgtcag ctccttcgac tatgccggcc cgctgcgagc gaccgccagc ccgctggtga
     1441 gcgccgacgg aggagcgtca ctgaccgaga tcaccacgcg gttcgtcggg tgcgtcccaa
     1501 gccccaagaa cacgcccggc tggccggatg tgctccgggc cgctcccgcc agcttccccg
     1561 atccgctcct tctggacccg gaggtcgccc tggagccccg gcagacccag tcggtgtggg
     1621 tcacggtgca cgtgcccgcc cacgccgcgc ccggcgagta ctcggggagc atcacccttt
     1681 ccactgccga tgcgcccgcg accgtggtgc ccctccgctt gcgggtgtac aggccggctc
     1741 tgcccaacgt ccccagcttc cagatggggt cctggggcgg ggacacgttc ctggccaacg
     1801 aggcggaagg caagccgcgg gacgacaagt catacgacaa tgcgtacttc gcgttcctga
     1861 aggacgagtg cctgcgcgac cgcttcgagc accgggcacg ggtcttctcc gacgtgcccc
     1921 tgtggagcgt ctttggcacg tgcggtgtct cctggcgaga cggccagtac gccttcgact
     1981 acacgctctt tgaccgcttc atctcgacgg tcgaggaggc gatgcatggc agcttccgcg
     2041 tcatctgtgt tggtatcgcg gccggcacct ctgaggacaa gacgcagctt ctcagcggcg
     2101 acatcgcggt gacgaacgcc gacgggtcgc ccaatgcaga gcggtccttc gccaacgtgc
     2161 cgaccgatgc cccgcggtac cgggaattcc tcggggcctt catgcacgat atgcaggagc
     2221 acctgcgggc caaagggtgg cttgagaggg tctacttcaa ggtgttggac gaggtgtcgg
     2281 gtgccacag
//
LOCUS       DAOSPL010000789         2288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47062_length_2288_cov_3.751008, whole genome shotgun sequence.
ACCESSION   DAOSPL010000789 DAOSPL010000000
VERSION     DAOSPL010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47062_length_2288_cov_3.751008"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PLQ54_13705"
     CDS             <1..717
                     /locus_tag="PLQ54_13705"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS94369.1"
                     /translation="PCHPDALTAESARTLRAQAEDAGLAVSALAAHTTWVLPGPDASW
                     ALEYMKRCVDAAVALGAPYVVTGTGPFPRDTDRFAARDALVGALKEAAEAANGSGVVL
                     ALEPHVGHMGMTWESVLGLLTTVDADALRVNFGAGHYFALGLDDRAALRQLSAYVVHV
                     HLRDYRRHQRPLCDLQLDPANLEAVALGEGDYDVAGHLDDLDAWGFDGVCSAELFVPD
                     VRAALKRSAQHIQPLLERIG"
     gene            759..1658
                     /locus_tag="PLQ54_13710"
     CDS             759..1658
                     /locus_tag="PLQ54_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94370.1"
                     /translation="MSKPVWRGLSCVLVLGAASAAISQTMTPTATPAAWRLAEVGTLR
                     GFDVPECVVVDPATGSAYVSNIQTQPGMGWEDDGAAFLSRLRPGGALSTLHWNAAALE
                     GKLNGPKGMCILGRGLYVADNTRVVRVSLTDGSVRPPIEPPGAQRLNDMATDGEAVYA
                     SDTATGNVFRIAVDGSTRVIQGPPMINGITFAGKRLFAVSWEPAGLWELDPSGKLPPR
                     SLGLPGRFVHLDGIEVLEDGTFIISDFHGNAIYAVGPDGAEVSKLAEVGTPADIGIDR
                     HRGLLFVPQFDHNQVVVYRIERD"
     gene            complement(1655..>2288)
                     /locus_tag="PLQ54_13715"
     CDS             complement(1655..>2288)
                     /locus_tag="PLQ54_13715"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOS94371.1"
                     /translation="QFETVFRLSVAAEFRDTETAAHIQRMSRYTEIIARNLGLEARDV
                     ELARFASPMHDVGKLGVPDSILLKPGRLSDDETAVMRSHTIIGARILDGSESALLQAS
                     ETIALTHHERVDGLGYPLGLKGDKIPLLGRIACLADALDAMTSRRCYKEAVSLEAGME
                     TCRHEAGHHFDPECVQALEKGFTEVSAVHRQFADSARDLEALVRRLRLVS"
BASE COUNT          368 a          776 c          752 g          392 t
ORIGIN      
        1 ccttgccacc ccgatgcact tactgcggag agcgcccgta ccctccgggc acaggccgag
       61 gacgcaggac tggctgtcag cgccctcgcg gcgcatacga cctgggtgct ccccggccct
      121 gatgcgtcgt gggcgctcga gtacatgaag cgctgcgtgg atgccgccgt ggccctgggc
      181 gcgccctacg tggtaacagg caccggaccc ttcccgcgcg atacggaccg gttcgccgcc
      241 cgtgatgccc tggtgggtgc cctgaaggag gccgccgagg cggccaatgg atctggggtg
      301 gtcctggctc tcgaacctca cgtgggacac atgggcatga cgtgggagtc cgtgctcggc
      361 ttgctgacca cggtcgacgc cgacgcactc cgcgtgaact tcggtgcggg acactacttc
      421 gccctgggcc tggacgaccg cgccgccctg cgccagctct ctgcctacgt ggtgcacgtt
      481 cacctgaggg actatcggcg ccaccagcgt ccactgtgcg atctgcagct cgacccggcg
      541 aatctcgagg ctgtggccct gggtgagggc gactatgatg tggccgggca cctcgatgac
      601 ctggacgcat ggggcttcga tggcgtctgc agcgcggagc tcttcgtacc ggacgtccgc
      661 gccgcgctca agcggagcgc ccagcatata caaccgctgc tggagcgcat cgggtagcat
      721 atcgctcgct tggacagcat ccctggaggc gaatacacat gtcgaaacct gtctggcgcg
      781 gcctgagctg cgtgctggtc ctcggcgcgg cgagcgccgc gatatcccag acgatgacgc
      841 ccacggccac tcctgccgcg tggcgactgg ccgaagtagg caccctccgc ggcttcgacg
      901 tgcccgagtg cgtcgtggtc gaccccgcga ccggttccgc ctatgtctcg aacatccaga
      961 cacagccagg catgggctgg gaagacgatg gcgccgcctt cctctcacgg ctcaggccag
     1021 gaggggcgct gagtacgctg cactggaacg ccgcagccct cgaaggcaag ctcaacggcc
     1081 ccaaaggcat gtgtatcctc gggcgtgggc tctacgttgc tgacaacacg cgggtcgttc
     1141 gcgtgagtct gaccgatggt tccgtgcgac cacccatcga gccccccggc gctcagcgac
     1201 tgaacgacat ggccaccgac ggcgaagcgg tctatgcgtc cgacaccgcc acaggcaacg
     1261 tcttccgtat tgccgtcgac ggctccacac gtgtgatcca gggacccccg atgatcaacg
     1321 gtatcacctt tgccgggaaa cgcctgttcg ctgtgagctg ggaaccggcc ggtctgtggg
     1381 aactggaccc ttccggcaaa ctgccgccga ggtccctggg cctgccgggc cgtttcgtgc
     1441 acctggatgg catcgaggtc ctcgaggacg gcaccttcat catctcggac ttccatggca
     1501 acgccatcta cgccgtgggg cccgatggtg ccgaggtctc gaagctcgcc gaggtcggta
     1561 cgcccgccga tatcggcatc gatcgccacc gggggctgtt gttcgtcccc cagttcgacc
     1621 acaaccaggt agtggtgtac cgtatcgagc gcgactagct aaccagccgg agccggcgaa
     1681 ccagagcctc gaggtcgcgg gcgctatcgg cgaactggcg gtggaccgcg gagacctcag
     1741 tgaacccctt ctcgagggcc tgtacgcact ccggatcgaa gtggtggccg gcctcgtggc
     1801 ggcatgtctc catgcccgcc tccagcgaga cggcctcctt gtagcagcgc ctcgatgtca
     1861 tcgcgtcgag cgcgtcggcg aggcacgcga tgcggcccaa cagcgggatc ttgtcgccct
     1921 tcaggcccaa cgggtatccc agaccgtcca cccgttcgtg gtgggtcagc gcgatggtct
     1981 cggacgcttg cagaagcgcc gactcgctcc catccaatat gcgggcgccg atgatggtgt
     2041 gagaccgcat cacggccgtc tcgtcatcgg agagccgccc gggcttgagg aggatcgagt
     2101 cgggcacgcc gagtttgccc acatcgtgca tggggctcgc gaaccgggcg agttcgacat
     2161 cgcgcgcctc gaggcccagg ttccgggcga tgatctcggt atagcggctc atgcgttgaa
     2221 tgtgcgccgc cgtctcggtg tcgcgaaact cagcggccac cgacaggcgg aataccgtct
     2281 cgaactgc
//
LOCUS       DAOSPL010000790         2285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47155_length_2285_cov_3.482511, whole genome shotgun sequence.
ACCESSION   DAOSPL010000790 DAOSPL010000000
VERSION     DAOSPL010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47155_length_2285_cov_3.482511"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="PLQ54_13720"
     CDS             <1..994
                     /locus_tag="PLQ54_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94372.1"
                     /translation="PYCPSDEAYARVLEFVRRGGALCLSGDVSYGPDRRRTRTRRLEE
                     LCGVRFVAERYPNIEYHAGEGGEARAETDDPLARTIAASGLTAPCIEVEPTESQVLAR
                     AGGVPLAVLNRVGAGRVLYLTDPAELHSEPVNVFRVFLDSAGVRRHALTPDLDTLRSH
                     RVAGEDGAVAHVLLNLSDATQRVEVADLPQPVAIHLAPRSAGAAVFDRRGRLVAMEGT
                     RVEVSGRVLLRADAPAIVIALDDDVDLRDARRVVALPSAPGVLELPTLDAATVASVGE
                     IVGGHWVEHERIPAARGRLNLDLAMAHSWIAMVPEDDARDGGRSIVARCLQLRR"
     gene            1039..1962
                     /locus_tag="PLQ54_13725"
     CDS             1039..1962
                     /locus_tag="PLQ54_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4438 domain-containing protein"
                     /protein_id="HOS94373.1"
                     /translation="MPAPLKTNVERLLRISVEVQPVHPAQPAIYRVGPDGSVHVGPGV
                     GGITLNLKVGDPALGWVADHAEPCVTTRNTDNGRNSGVAFLACIGNLATVISGDAKGD
                     QGVVTGKHGGVEHTMIDFADDTLAKLAYGDTIRIDAYGQGLALLDAGDIIPMNLDPGL
                     LTHPKWGVKLSRGRMTVPVAHVVPAKLMGSGLGRDNSRGGDYDIQLFDPALVEEHGLA
                     SLRLGDIVAITDADHSFGRIYRTGAVSISVITHGACTTAGHGPGATTLLTSATGAIVP
                     VCDAKANLADILGVGRERRARRKPRRPSGKK"
     gene            2009..>2285
                     /locus_tag="PLQ54_13730"
     CDS             2009..>2285
                     /locus_tag="PLQ54_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94374.1"
                     /translation="MITDRKILPKLEHIERQYSALRFEKVAEVSAEMCETREHYRSEP
                     GPSSKLKWRPAAPGAQWGDSGMTAWFRADVKLPKACTGRRVFVRARTG"
BASE COUNT          358 a          767 c          778 g          382 t
ORIGIN      
        1 tccctactgt cccagcgacg aggcctatgc gcgggtgctc gagttcgtcc gcaggggagg
       61 cgcgctctgt ctctcgggcg atgtgtccta tggtccggac cgtcgaagga cgcgcactcg
      121 acgcttggag gagctctgcg gggtccggtt cgtggccgag cgctatccga atatcgagta
      181 ccacgccggc gagggcggtg aggcgcgggc cgagacggac gatccgctgg cgcgcaccat
      241 cgcagcttcg ggcctgactg ctccatgcat cgaggttgag ccgacggagt cgcaggtgct
      301 cgcccgcgcc ggaggggtgc cactggccgt gctgaatcgc gtgggcgctg gtcgagtgct
      361 ctacctgacg gatccggctg aactgcacag cgaaccggtg aacgtgttcc gggtctttct
      421 cgattccgcc ggcgttcgcc gccacgcgct gacccccgac ctcgataccc tccgctcaca
      481 tcgcgtggct ggcgaggacg gggccgtggc tcacgtgctc ctgaacctct cggatgccac
      541 gcagcgggtg gaagtggcag acctgcccca gcctgtggcc atccatctcg ctccgagatc
      601 cgcgggtgcg gcggtattcg accgtcgcgg ccgtctcgtt gcgatggagg gtacccgcgt
      661 ggaggtcagc ggccgcgtgc tcctgcgtgc ggacgctccg gccatcgtga tcgccctcga
      721 tgacgacgtc gacctgcgcg acgcccggag agtggttgca ctgccctcag ctcccggcgt
      781 gctcgaactc ccgacgctcg atgccgccac tgtggcatct gtcggcgaga tcgttggcgg
      841 gcactgggtc gagcatgagc gcatcccggc cgcccggggc cgtctgaacc tcgatctagc
      901 catggcgcac tcatggattg ccatggtccc ggaggacgat gcccgcgacg gcggaagatc
      961 catcgtggcc cgttgccttc agttgcgccg ctagggtgcg ggctcgatga tgtgggacaa
     1021 tcggggaggt accgccacgt gcccgcgcca ctgaagacga acgtcgaacg gctgctccgg
     1081 atcagcgtcg aagtccagcc ggttcatccc gcccagcccg cgatctaccg cgtcggtccc
     1141 gatggttcgg tccacgtcgg ccccggcgtg ggcggcatca cgctcaacct caaggtcggt
     1201 gacccggccc tgggttgggt cgccgaccac gctgagccct gtgtgacgac ccgcaatacc
     1261 gacaacggcc gcaattccgg tgtggcgttc ctcgcttgca tcgggaacct ggctacggtg
     1321 atcagtgggg acgccaaggg cgatcagggc gtcgtgactg gcaaacacgg tggagtcgag
     1381 cacaccatga tcgacttcgc cgatgatacc ctcgccaagc tcgcctacgg tgacaccatc
     1441 cgcatcgatg cctacggcca gggcctcgcg ctgctcgacg ccggtgacat catccccatg
     1501 aatctcgatc ccggtctgct tacccacccg aagtggggcg tcaagctcag caggggccgc
     1561 atgacggtcc cggtggctca cgttgtgccg gcgaagctca tgggctcggg cttgggcaga
     1621 gacaacagtc ggggcgggga ctacgacatc cagctgttcg accccgcact ggtcgaggag
     1681 catggcctcg cgagcctgcg tcttggcgat atcgttgcca ttaccgatgc cgaccattca
     1741 ttcggccgca tctaccggac cggagcggtg agcatcagtg tcatcaccca cggcgcgtgc
     1801 acgacggccg gccacggccc aggggccacc acgctgctca cgtcggccac gggggccatc
     1861 gtgccggtct gtgacgccaa ggccaacctg gcggacattc tgggcgttgg gcgagagagg
     1921 agggcgcgga ggaagccgcg ccggccgtcc ggcaagaagt agggtcccgc gctccgggcg
     1981 cgttgcgagt agaggagagc ctgccaccat gatcaccgac cgtaagatcc tccccaagct
     2041 cgagcacatc gagcgccagt acagcgcact gcgcttcgag aaggtcgccg aggtctcggc
     2101 cgagatgtgt gagacccgcg agcactatcg ttccgagccc ggcccatcct ccaagctcaa
     2161 gtggcgcccg gcggccccgg gggcccagtg gggcgacagc ggcatgacgg catggtttcg
     2221 ggccgatgtg aagctgccca aagcttgtac cggtcggcgc gtgttcgtcc gcgctcgaac
     2281 cggcg
//
LOCUS       DAOSPL010000791         2283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47215_length_2283_cov_3.267504, whole genome shotgun sequence.
ACCESSION   DAOSPL010000791 DAOSPL010000000
VERSION     DAOSPL010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47215_length_2283_cov_3.267504"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..1370)
                     /locus_tag="PLQ54_13735"
     CDS             complement(174..1370)
                     /locus_tag="PLQ54_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS94375.1"
                     /translation="MATDLEQRYDQRLTRYVTALRNEKADCVPIRPFLAEFCAKYTGY
                     TAQEVTHDFTKAFDAVLRTATDFDFDAMVGNMVYVWTGLTQAIGLEYYAIPGIDVEPD
                     TGFQYREPSEERAYMKPEEYDALIEDPTGFLLNVWLPRVSRDVVPIGAPSTFRSNLSF
                     LKGGMAMMQYFAGFGTQAQRMRTECGMPGAIAGILKAPFDILADKLRGFLGLTMDLFE
                     RPDKVRAACEALMPHLTHVALSGADPAKQVPITIWMHRGCTPFISPAQFDGLYWATLK
                     PIIEEIWSRGHQVLFYAEGNWDRHLPAFAELPEASIIYHVDMGDILEAHRVLGHKFCI
                     SGGMPNFLLSYGTPDQVRERCKALIERVGRDGGYVMDASAIVQNDARVENVRAMVEAT
                     REYGTY"
     gene            complement(1421..2098)
                     /locus_tag="PLQ54_13740"
     CDS             complement(1421..2098)
                     /locus_tag="PLQ54_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOS94376.1"
                     /translation="MARATYLGHAAVRIESAPHAVIIDPFLTGQRSPVRPEDLRVDAV
                     LVTHGHGDHVGDTVAIAQANSAWVVAPSELAGWLARQGVKTHAMHIGGAHQFDFGWVK
                     LTPALHGSAAMGESTVYTGNPCGFLVRMGGRLIYHAGDTGLSAEMELIGRRNEIHLAF
                     VPVGDNYTMGVDDAAEAVRMLRPRRVVPMHFGTFPGIEADTDAFARAVGPAAEVVVLQ
                     PGETTEA"
BASE COUNT          391 a          758 c          738 g          396 t
ORIGIN      
        1 tggcagtgcc agatgtacgt gtacgcgagg gagtccacgt cctcccagac ccgtcgacac
       61 agatcggcgt caccccggat ctccggaagc tccttcagct tctcctgcca cgggatgcac
      121 acaccgggtt cccggtgaat ctcggtgggc tgatccacgg gccgctcccc ccatcagtac
      181 gtgccgtatt cgcgcgtggc ctcgaccatg gcccgcacgt tctcgactcg cgcgtcgttc
      241 tgcacgatgg cactcgcatc catgacatac ccgccgtcac gaccgacccg ttcgatcagg
      301 gccttgcagc gctcgcgaac ctggtcgggc gtgccatagc tgagcaggaa gttgggcatg
      361 cctccgctga tgcagaactt gtgcccaagt acgcggtgcg cctccaggat gtcgcccatg
      421 tccacgtggt agatgatgct cgcttcgggc agctccgcga aggccggcaa gtggcggtcc
      481 cagttaccct cggcgtagaa caacacctgg tgcccgcgcg accagatctc ctcgatgatg
      541 ggcttcagtg tggcccagta gagaccgtcg aactgagccg gcgagatgaa cggcgtgcag
      601 ccccggtgca tccagatggt gatgggcacc tgcttcgccg gatcggcacc cgatagcgcg
      661 acatgggtca ggtggggcat cagggcctca caggcggccc ggaccttgtc cggcctctcg
      721 aacagatcca tggttaggcc gaggaacccg cgcagcttgt cggccaggat gtcgaacggg
      781 gccttgagaa tccccgcgat agctccgggc atcccgcact cggtccgcat ccgctgagcc
      841 tgagtgccga accccgcaaa gtactgcatc atagccatgc cgcccttgag gaaggacagg
      901 ttactgcgga aggtcgatgg ggcgccaatc ggcacgacgt cgcgcgagac ccgcggcagc
      961 cacacgttga ggaggaagcc ggttggatcc tcgatgagcg cgtcatactc ctccggcttc
     1021 atgtaggctc gctcttccga tggctcccga tactggaacc cggtatccgg ttccacatcg
     1081 atacccggga tggcgtagta ctcgaggccg atggcctgcg tcagacccgt ccacacatag
     1141 accatgttgc cgaccatggc gtcgaagtcg aaatccgtgg cggtccgaag caccgcgtcg
     1201 aatgccttgg tgaagtcgtg ggtcacctct tgggccgtgt accccgtgta tttggcgcag
     1261 aactccgcca gaaatgggcg gatgggcacg cagtccgcct tctcattccg cagggctgtc
     1321 acataacgcg tcaggcgctg gtcatagcgc tgctcgaggt cggtcgccat gcaagtccac
     1381 ctctcggggg gtgaatccgt caggcgagtg acgccgccac tcaggcttcc gtggtctccc
     1441 cgggctggag gacgacgacc tcggccgccg gccccaccgc ccgcgcgaat gcatccgtgt
     1501 ctgcctcgat gccggggaaa gtcccgaagt gcatggggac gactcgacgg ggccgcagca
     1561 tccgcaccgc ctcggcggca tcgtcgacgc ccatggtgta gttgtcgccc accggcacga
     1621 aggccagatg gatctcattc cgccggccga tgagctccat ctcagccgag agcccggtgt
     1681 cgccggcgtg gtagatgagc cttcctccca tgcggaccag gaaaccacac gggttgccgg
     1741 tgtagacggt actctctccc atcgcagccg acccatgaag ggcgggcgtc agcttcaccc
     1801 atccaaagtc gaactggtgc gcgccaccga tgtgcatggc gtgcgtcttc acgccctgcc
     1861 gggcaagcca tccggcgagc tcggaaggcg ccacgaccca cgcgctgttg gcttgggcga
     1921 tggccaccgt gtcgcccacg tggtcgccgt gcccgtgggt gaccagcacg gcgtcgacgc
     1981 gcagatcctc ggggcgcacg ggggagcgct gacctgtgag aaatggatca atgatcacgg
     2041 catgcggcgc ggactcaatc cggacggctg cgtgtcccag gtacgtggcg cgtgccacac
     2101 tgcatcgcct cccccatggg cgaggtcgtt gaacgcatcc acttactcgt gtggcggatg
     2161 gtctcccggc gcgatggggc cgctctggcc gtcgaactcc cagtcgggcg cgtcggtctc
     2221 ggaggggtag aggagccgcg aggacgcctc gtcgaggatg accgcgtgcg cggaaagcgg
     2281 atc
//
LOCUS       DAOSPL010000792         2283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47217_length_2283_cov_2.602783, whole genome shotgun sequence.
ACCESSION   DAOSPL010000792 DAOSPL010000000
VERSION     DAOSPL010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47217_length_2283_cov_2.602783"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PLQ54_13745"
     CDS             complement(<1..457)
                     /locus_tag="PLQ54_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94377.1"
                     /translation="MVGIWLGSALVIGAATPSAAGESRSDDAVVRWTDRTIELETRAV
                     LLRLDMPAAGGLRRSAWVNRSTGVDVVAGGTQADFSVTVDGQLLSSTDASFVVKSVAC
                     QRTEHGALHVTLRVAHPKVGIARHYVVHPGIGLIRGWLEISNVARTPVRV"
     gene            639..1796
                     /locus_tag="PLQ54_13750"
     CDS             639..1796
                     /locus_tag="PLQ54_13750"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOS94378.1"
                     /translation="MPTRVFDDRDLGLVREVLQSGHLSILDGGQLTPRFQKAFAEAHG
                     AKHGVAMNCAMSVLHASVISAGAGAGDEVICDPVCVFGAMATMYANAIPVFVDVNPVT
                     WNMDPDAIEARITERTKALIVTHVCGLPAEIDRIVEVAHRHGIVVIEDCAHALLTRYK
                     GKCVGTWGDIGSFSFQASKQMGLGDGGMALTDNDELAGKLNLHAGAPTFMSVAYGMHY
                     NYRMSEPVAAIGIAQLERLPGYIEGLRRAAAMLDAAVADCEFIDLQRGPAEAEHSFHL
                     WGATFHGERIGLPRDDFARVLEEEQVGIGMGYTNMPAYRHPLIAERLGAGRGCPLDCP
                     LYEGTGNQYPDGLCPKVEALFPNIILGYPFQPDDAVAAQADRLRRAVERLA"
     gene            1853..>2283
                     /locus_tag="PLQ54_13755"
     CDS             1853..>2283
                     /locus_tag="PLQ54_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94379.1"
                     /translation="MRVAWVYDAQAHSDANAAGRNYWPVYTEEILRRLGITPERLAPA
                     DARDLIRLAGYAVVVAGSHPGVSPTVPSAAEAWVRAGGILIGFATVGLEELFGIRVEG
                     CLKQPEGDFGIAAYLRLTDCNLASDVHPAGFEDQPLLVVSPV"
BASE COUNT          374 a          779 c          754 g          376 t
ORIGIN      
        1 ccacgcgcac gggggtcctg gcgacattgg agatctccag ccacccccgg atcaggccaa
       61 tgcccggatg aaccacatag tggcgcgcga tgcccacttt gggatgcgcg acgcgaagcg
      121 tgacatgcag cgcaccgtgc tcggtgcgtt ggcacgcaac gctcttgacg acgaacgatg
      181 catcggtcga ggacaacagc tgcccgtcga ccgtgacgga gaagtcggcc tgtgtcccgc
      241 ccgcaacgac atccacgccc gtggatcggt tcacccacgc cgaccggcgc aagccaccgg
      301 cggctggcat gtcgaggcgc agcaggacag cgcgtgtctc gagttcaatg gtccggtccg
      361 tccaacgaac caccgcgtcg tccgagcggg actcccccgc tgcggatggc gtcgccgccc
      421 cgatcacgag ggcactaccc aaccagatac cgaccaaatc gagccccacc tttcgcatcg
      481 acggatcgcc gcggagcgga gcggggtccc cttcgcctgg tgcgccacca tgccctgcgc
      541 ccgccgcagg ctcggcaccc cgcaggaact ccacgcggag gggcagaagc cccgctcatc
      601 cggatcgtcc ggacaccccg cacccttcgg aggaccccat gcccactcga gtgttcgatg
      661 accgagacct cggtctggtt cgcgaggtgc tgcagtcggg gcatctctcc atcctcgatg
      721 gcggccagct taccccgcgc tttcagaagg ctttcgctga ggcccatggc gcgaagcacg
      781 gtgtggccat gaactgtgcc atgtcggtgc tgcatgcctc cgtcatctcg gctggagccg
      841 gggcggggga cgaggtcatc tgcgatcccg tgtgcgtgtt cggcgccatg gccacgatgt
      901 atgcgaacgc cattcccgtc ttcgtggacg tcaacccggt gacgtggaac atggatcccg
      961 acgccatcga ggcgcgcatc accgagcgga ccaaggcact catcgtgacc cacgtctgcg
     1021 gtctacctgc cgagattgac cgcatcgtcg aggtggcaca ccggcatggc atcgtggtca
     1081 tcgaggactg tgcccatgct ctgctgacac ggtacaaggg caagtgcgtc ggcacgtggg
     1141 gggacatcgg gtccttcagc ttccaagcca gcaagcagat gggtctgggt gatggcggta
     1201 tggcgctgac ggacaacgat gagctcgcgg ggaagctgaa cctgcatgcg ggggcgccga
     1261 cgttcatgtc cgtggcatat gggatgcact acaactaccg gatgtccgaa cccgtggccg
     1321 ccatcggcat cgcccagctc gagcgcttgc cgggctacat cgagggcctc cgccgcgccg
     1381 ccgccatgct ggatgctgcg gtggccgact gcgagttcat cgacctccag cgagggccgg
     1441 ccgaggccga gcacagtttc cacctctggg gcgctacctt ccacggcgag cggataggcc
     1501 ttccccgtga cgacttcgcc cgggtgctcg aggaggagca ggtcggcatc ggaatgggct
     1561 atacgaacat gccggcctac cgtcacccgc tcatcgccga acggctgggc gccgggcggg
     1621 gctgcccgct ggactgccca ctctacgagg gcacgggcaa ccagtacccg gatgggttgt
     1681 gccccaaggt ggaagccctg ttcccgaaca tcatactggg ctacccgttc cagcccgacg
     1741 acgccgtcgc cgctcaggcc gatcggcttc gccgggccgt cgagcggctc gcataggttc
     1801 agcccagttg cgagaggcag tgagcccctt ggttggctcc cccaggaggc cgatgcgagt
     1861 cgcgtgggtg tacgacgcgc aggcgcacag cgatgcgaac gcagccggcc gcaactactg
     1921 gccggtgtac acggaggaga tcctgcggcg tcttggtatc acgcccgaac gtctggcccc
     1981 ggccgatgcg cgggacctga tccgtctggc cgggtatgct gtggtcgtgg ccggctccca
     2041 tccgggcgtc tcgcccacgg tcccgtcagc cgcggaagca tgggtccggg ccggggggat
     2101 actcattggc tttgcgacgg ttgggctcga agagctgttc ggcatccgcg tggagggctg
     2161 cctgaagcag cccgaaggcg actttggcat cgcggcctac cttcggctca ccgactgcaa
     2221 cttggcctcc gatgtgcatc ccgccgggtt cgaggaccag ccgctcctcg tggtttcccc
     2281 cgt
//
LOCUS       DAOSPL010000793         2280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47292_length_2280_cov_3.029213, whole genome shotgun sequence.
ACCESSION   DAOSPL010000793 DAOSPL010000000
VERSION     DAOSPL010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47292_length_2280_cov_3.029213"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PLQ54_13760"
     CDS             <1..825
                     /locus_tag="PLQ54_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94380.1"
                     /translation="GQRVKLDLPKARIDTEVEAAGAQALGRAGETPVAFVHRAGAGAA
                     ALLNFQLLAGDAEQVKASRELARLVLSLSGARSPISVSAPGGGPLPNTESRVWRDGDA
                     LVFGLWRRMENEWFGPKSGTTAGPPVPASVTLPEPMHVYDLRAGKHLGNVKQFETTLR
                     WGRASFFMALPYEIAAPSVTVSPRMPVPGEPATAEIELAVPPRASEKLAVFVQAVAPD
                     GSTPFWGQKVVLLDGGRGQVQFPTPYNPVVGRWKVKATELFSGKSGEASWTLREAM"
     gene            828..2000
                     /locus_tag="PLQ54_13765"
     CDS             828..2000
                     /locus_tag="PLQ54_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94381.1"
                     /translation="MSPDRCVRLVALALPLLAGPAPGADEPPRVDVSDTGAAITPATW
                     ECTAGHPIAKRTISLQSGAANYAFLIAGCQDPSHDGKHPCSEGNFGMPSPSSANWYWG
                     GFLRVMVNGSDATVCDLRDVRVLERGPRGSFQVIWAHPDADVGLRLMMLGGENHVDAD
                     VVWKPREGATVQSISLQLTCYPSFFTAARHRQGERHCRTPRIDQKEPSTVEVQPGEDT
                     FLYYYDAVFDVAKGEGDGPCAVVLDPAGIAGGSVSIGNYAVQTTLNLKPEAGQARLAL
                     YDFTGKTNADAEAYLRDHGAADQVALSKADFRPEPVRGLQPNQLRTDALNLLALAADD
                     GQALEPRVMELLTKVDALKAAADQGDWIAEADLSTALLDSVDLFWKLQTFAALNAE"
BASE COUNT          369 a          801 c          770 g          340 t
ORIGIN      
        1 ggccagcggg tgaagctcga cctgccgaaa gctcgcatcg acaccgaggt cgaggcggcc
       61 ggtgctcagg cgctgggacg ggccggcgag acgcccgtgg cattcgtcca ccgcgccggc
      121 gccggtgcgg cggccctgct caacttccag ctcctcgccg gcgatgccga gcaggtgaag
      181 gcgtcgcgcg agctggctcg tctggttctg agcctgagcg gagcgcgatc gcccatcagc
      241 gtgagcgcgc ccggcggcgg gccgctgccg aacaccgagt cgcgcgtgtg gcgcgacggc
      301 gatgcgctcg tgttcggcct ctggcggcgc atggagaacg agtggttcgg gcccaagtcg
      361 ggcaccaccg ccggaccgcc ggtgccggca tcggtcacgc tgcccgagcc catgcacgtc
      421 tacgacctcc gcgccggcaa gcacctcggc aatgtgaaac agttcgagac caccctgcgc
      481 tggggccggg ccagtttctt catggccctg ccttacgaga tcgccgcgcc gagcgtcacc
      541 gtctcgcccc ggatgccggt cccgggcgag ccggctacgg ccgagatcga actggccgtg
      601 cccccccgcg ccagcgagaa gctcgccgtg ttcgtccagg ccgtggcgcc cgacggcagc
      661 acccccttct gggggcagaa agtggtcctg ctcgatggcg gccgcggtca ggtgcaattc
      721 cccacgccct acaacccggt ggtcggccgg tggaaagtca aggcgaccga gctgttcagc
      781 ggcaagtcag gcgaggcgag ctggacgctg cgggaggcga tgtgacgatg agtcccgacc
      841 gatgcgtacg actggtggcc ctcgcactcc ccctcctcgc cggcccggcg cctggtgccg
      901 atgaaccgcc ccgggtggac gtgtccgaca ccggcgccgc catcacgccg gccacctggg
      961 agtgcacggc cgggcacccc atcgccaaac ggaccatctc gctccagtcc ggggccgcca
     1021 actacgcgtt cctgatcgcc ggctgccagg atccctcgca cgacggaaaa cacccgtgtt
     1081 ccgagggcaa cttcggcatg ccctccccct ccagcgccaa ctggtactgg ggcgggttcc
     1141 tgcgcgtcat ggtcaacggc tccgacgcca ccgtttgcga cctgcgcgat gtgcgcgtgc
     1201 tcgagcgcgg cccgcgggga tcgttccagg tcatctgggc gcacccggat gccgacgtgg
     1261 gcctccgtct gatgatgctg ggcggcgaga accacgtcga tgcggacgtc gtctggaagc
     1321 cccgcgaggg tgccacggtg caatccatca gcctgcagct cacgtgctac ccaagcttct
     1381 tcaccgccgc ccggcaccgg cagggcgagc ggcactgcag gacgccgcgc atcgaccaga
     1441 aggagccctc gacggtggag gtccagccgg gagaggacac cttcctgtac tactatgacg
     1501 ccgtgttcga cgtggccaag ggcgagggcg acggcccgtg tgcggtggtg ctcgacccgg
     1561 cgggcatcgc gggcgggagt gtctccatcg gcaactacgc ggtgcagacg acgctgaacc
     1621 tcaagccgga agccggccag gcgcggctgg cgctgtatga cttcacgggg aagaccaacg
     1681 ccgacgccga ggcgtatctg agggaccacg gcgcggccga tcaggttgca ctcagcaagg
     1741 ccgacttccg ccccgagccc gtccgcggtc tgcagccgaa ccagctccgc accgatgcgc
     1801 tgaacctgct ggccctggcc gccgatgacg gccaggcgct ggagccgcgg gtgatggagc
     1861 tgctgaccaa ggtggatgcc ctgaaggccg ccgcagacca gggcgactgg atcgccgagg
     1921 ccgatctctc aacggcgctc ctcgactcgg tggacctgtt ctggaagctg cagaccttcg
     1981 cggcgctgaa cgccgagtag gggcccgacc ggcgtcacag gtaagcttat atgctcatat
     2041 ggccacttca ctcccccaaa tggccccttt tcgtccccat cgccctacac aactggccaa
     2101 gcccgggctc ctccgggccg ccggacgggc gcgaaatggt aagggccggt aagggccttc
     2161 tccgcgggtg cagaggtgcc acggaggccc tccaggcaaa ggtatatacc tatcgctgca
     2221 aaaggtgact tcatagccgt actggtgttc gtatactatg atcagcctca acaatcaatg
//
LOCUS       DAOSPL010000794         2278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47333_length_2278_cov_2.843455, whole genome shotgun sequence.
ACCESSION   DAOSPL010000794 DAOSPL010000000
VERSION     DAOSPL010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47333_length_2278_cov_2.843455"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..1635
                     /locus_tag="PLQ54_13770"
     CDS             265..1635
                     /locus_tag="PLQ54_13770"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOS94382.1"
                     /translation="MPATAPAWAQIDPLTVPHIVTDTIPGPRSEQLNARAMRHMIGYS
                     SQVKLCPVAFESGHGVTLTDVDGNRYIDFSSGIYVTTLGHCHPKVSEAVARHAHSLMN
                     CHDFNTPVKAALLEKLAQIQPWDLTGMQLYDSGTTAVEAGLRVCRAATGKTEFISCFM
                     DFHGKTGHAVSLGKMNATNGPGRSQGFYMVPRPYPYRPLFTRADGTIDTDAYIDFYAE
                     FIDNATTGNVAAFVLEPVQGWAGSVVPPDDFFPKLRSLCQERGILLMADEVLTSMGRT
                     GAYLCMEHWDTPADVVTLGKGFGNGFPVTAMLVSERYRESIDKISASTSYGGNPMACA
                     AALASIEAIEEEGLLENALQLGDFFIQRMEALKAAHPLVGDVRGIGCLLGIELVKDRS
                     TKEPFTEAGQRVYRRAFEKGLAWVPAGHILRMSPPIVMEEAVAGRGMDIIEEAIAEVE
                     AELGYA"
     gene            1628..>2278
                     /locus_tag="PLQ54_13775"
     CDS             1628..>2278
                     /locus_tag="PLQ54_13775"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOS94383.1"
                     /translation="MPEPLFLSFDLGTTALKTALVDVHGRIRGLHTREYTYHTPQPDR
                     AEMPAEEYLAAAAEGARAVIHDAGCGPGDVSAIGLSSQGETFVALDGAGRPLGPAIVW
                     VDQRALGIAAEWEANGLDRRAYRRIAGYAWLPCELSAFKIGWLRRHAPEMHRAARFLC
                     LPDWVAFWLTGEAATDYTIALMSGLFDLGAQHWSPEMLRRAGVGPEQLPRVLPPGTI"
BASE COUNT          394 a          841 c          691 g          352 t
ORIGIN      
        1 gcacacggcg gcatcggcgc agttcccgcc acgccggagc acgctcatac ccgcttcggt
       61 ggccaggtag ttcgaggtgg cgatcgcccc tctcagcccc cgcacggcac tccgagtcgt
      121 gatcgacagt ggcacgccgc aacaccccca tgagcccttc gggccatcgc ccgcgaaccc
      181 cttgccgccc ctgcggtccg caggactagg ctcccgggag gacgaaggaa gcgaggcatc
      241 gcgtctcagg accggaggat agccatgccc gccactgccc ctgcgtgggc ccaaatcgac
      301 ccactcaccg taccccatat cgttaccgac accattcccg gcccgcgctc cgagcaactg
      361 aacgcacgcg ccatgcggca catgatcggc tactcgagcc aggtgaagct gtgcccggtc
      421 gcgttcgaga gcggccatgg ggtcacgctc accgacgtcg acggcaaccg ctacatcgac
      481 ttctcgagcg gcatctacgt gaccactctc ggccattgtc atcccaaggt cagcgaagcg
      541 gtggcacgcc acgcccacag cttgatgaac tgccacgact tcaacacgcc ggtcaaggcc
      601 gcactcctcg agaagctcgc ccagatccag ccatgggacc tgaccggcat gcagctctac
      661 gatagcggca ccacggccgt cgaggccggc ctccgcgtct gccgcgccgc caccggaaag
      721 accgagttca tctcatgctt catggacttc cacggcaaga ccggccacgc cgtgagcctg
      781 ggcaagatga acgccaccaa tggcccaggc cggtcccagg gcttctacat ggtgcccagg
      841 ccgtacccgt accgcccgct cttcacccgg gcagatggca ccatcgacac cgatgcctac
      901 atcgatttct acgccgagtt catcgacaac gcgaccaccg gcaacgtggc cgcctttgtg
      961 ctggagccgg tccagggctg ggccggctcc gtcgtgcctc ccgatgactt cttcccgaag
     1021 ctccgctcgc tctgccagga acgcggcatt ctgctgatgg ccgacgaggt gctcaccagc
     1081 atgggccgca ccggcgctta cctatgcatg gagcattggg acaccccggc cgatgtggtc
     1141 accctcggca aaggcttcgg caatggtttc cccgtcaccg ccatgctggt ctccgaacgc
     1201 tatcgcgaga gcatcgacaa aatcagtgcc tcgacgagct acggcggcaa ccccatggca
     1261 tgcgccgcgg cactggccag catcgaggcc atcgaagagg agggcctcct cgagaacgcc
     1321 ctgcagctcg gtgacttctt catccagcgg atggaggcgc tcaaagcggc ccacccgctg
     1381 gtgggtgatg tccgagggat cggctgcctg ctcggcatcg aactcgtcaa ggaccgttcg
     1441 accaaggaac ccttcaccga ggccgggcaa cgagtctatc ggcgggcttt cgaaaagggg
     1501 ctggcttggg tgccggccgg ccacatcctg cgcatgtcgc cgcccatcgt gatggaagag
     1561 gccgtcgcgg gccgaggcat ggacatcatc gaggaagcca tcgccgaggt ggaggccgag
     1621 ctcggctatg cctgagccgc tgttcctgtc ctttgacctc gggaccacgg ccctgaagac
     1681 cgccctggtc gatgtccacg gtcgcatccg cggcctgcac acgcgcgagt acacctacca
     1741 caccccccag cccgaccggg ccgagatgcc ggccgaggag tacctcgcgg cggccgccga
     1801 gggtgcccgc gccgtgattc acgatgccgg ctgcggacca ggagacgtct ccgccatcgg
     1861 cttgagctcc cagggcgaga ccttcgtggc actcgacggc gccgggagac ccctgggacc
     1921 cgccatcgtc tgggtcgacc agcgtgcgct gggcatcgcc gccgagtggg aggccaacgg
     1981 cctggaccgc cgagcctacc gccgtatcgc cggctatgca tggctgccct gcgagctctc
     2041 ggcgttcaag atcggctggc tccgccgtca tgcccccgaa atgcaccgag ccgcacggtt
     2101 cctgtgcctg cccgactggg tggcattctg gttgaccggc gaagccgcca ccgactacac
     2161 catcgccctg atgtccggcc tgttcgacct cggcgcgcag cactggtcac ccgagatgct
     2221 ccgccgcgcc ggcgtcggcc ccgagcagct cccacgcgtg ctcccgccag gaaccatc
//
LOCUS       DAOSPL010000795         2274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47465_length_2274_cov_3.431726, whole genome shotgun sequence.
ACCESSION   DAOSPL010000795 DAOSPL010000000
VERSION     DAOSPL010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47465_length_2274_cov_3.431726"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PLQ54_13780"
     CDS             <1..989
                     /locus_tag="PLQ54_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94384.1"
                     /translation="QADDAAVRELDTTRPPAVLLVCWADVMWREQEGERSSVYGLLLG
                     HEHEMPPRTDLTPAPSPTEAGPRSFASASWRERTARLRRVRACAPGSWQSNDYETVGP
                     DLAGKMTTGLVTLTRAAPSHPEELLRECLAHGVQLALTDALAQSDVLVALRAQPTEAA
                     LASLALTVSADLERTTDGVRGVPGNRSTYDWAIDTVGEVLRELARPCLDAGLAGTGVA
                     AVEADQIWQCLQWRPMDQVPDAERLVFDAARPFIAPGSLEVEPPPPDTAELAARTTFG
                     LWLGRVAEEWSAAGPTHGYVALDLTYVSGSSVGPFEPRVAAPELPLSAGETG"
     gene            1001..1531
                     /locus_tag="PLQ54_13785"
     CDS             1001..1531
                     /locus_tag="PLQ54_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94385.1"
                     /translation="MMGYRIAALVLALAGVGWSATCYEVDGSSWVQPEMAVPYTCDYD
                     YTTCNPYLHGPRLVTIDCSGHPKDPYNSAYACQRSELSECYYADGLTPVPPVLYVSAP
                     AEHWEAIVDPDDPVNRGKYRGVHTKHRGAATVQQHYRGMPMMMGQGFMVTDQCQSVSG
                     ICLWYTWQVVRDPCGP"
     gene            1812..>2274
                     /locus_tag="PLQ54_13790"
     CDS             1812..>2274
                     /locus_tag="PLQ54_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94386.1"
                     /translation="MPCIVPVQDDTGDGPPTWLRQAASFETTPGHEPRSAVSAISYWA
                     RGNGHEGFTTPHYVVMVPCPQRLGPDSLPGASYGIRESALAIAIATAPADVALHLACG
                     FKVIAQQLSEEAQATLVRSLIVNRSGHGYSRSLEKPLMLAVSLAPTLIWRPS"
BASE COUNT          381 a          744 c          764 g          385 t
ORIGIN      
        1 gtcaggccga tgacgccgcc gtcagggagc tcgacacgac caggcctccg gccgtcttgc
       61 tcgtctgctg ggctgacgtc atgtggaggg agcaggaggg agagcggtcg tctgtgtacg
      121 ggctgctact aggccacgag cacgagatgc cacctcggac cgacctgacc cccgcgccct
      181 cgccaacaga ggcgggcccg cggtccttcg ccagcgcaag ctggagggag aggaccgctc
      241 gcctccgccg ggtgcgggcc tgtgcgccgg gttcgtggca gtcgaacgac tacgagaccg
      301 tcggccctga cctcgccgga aagatgacta ctggcctggt cactttgacg cgggcggccc
      361 cctcgcaccc tgaggagctt cttcgcgagt gcctagctca cggcgttcag ctcgcgctga
      421 cagacgcctt ggcacagagc gacgtgctcg tcgcgctgcg ggcccagccc acagaggctg
      481 cgcttgcatc gcttgctctc accgtgtcgg ccgatctgga gcggaccacc gacggtgtgc
      541 gtggagtgcc agggaaccgg tcgacgtacg actgggccat cgacacggtg ggagaggtcc
      601 tcagagagct cgcacggccc tgcctcgacg cagggctggc tgggacaggt gttgccgctg
      661 tggaggcgga ccagatctgg cagtgcctcc agtggcggcc aatggatcag gtgcctgacg
      721 ctgagcgcct ggtgttcgac gccgcgcggc cgttcatcgc tccaggatca ctcgaggtgg
      781 agccgcctcc gccagacaca gctgaactcg ctgcaaggac gaccttcggg ctgtggctag
      841 ggcgcgtcgc ggaggagtgg tccgctgctg gcccaaccca tgggtatgtg gccctggatc
      901 tcacatacgt ctctggatcc agcgtggggc cattcgagcc ccgcgttgcg gccccggaac
      961 tgcccttgag cgcaggggag actggatagg aggatgcgac atgatgggtt accgaattgc
     1021 cgctctggtt ctggcgctgg cgggagtggg gtggagcgcc acatgctacg aggttgacgg
     1081 aagctcctgg gtccagccgg agatggccgt cccatacacc tgcgactacg actacaccac
     1141 atgcaatccc tacctgcacg ggcctcgtct cgtcacaata gactgctctg gccacccgaa
     1201 agatccctac aactccgcgt atgcctgcca gcgctcggag ttatcggagt gttactatgc
     1261 cgacgggctg accccggttc ctccggtcct ctatgtgtca gcgccggctg agcactggga
     1321 ggccatagta gacccggacg acccggtcaa ccgaggcaaa tacaggggcg ttcacacgaa
     1381 gcaccgcgga gccgccacag tccagcagca ctaccgaggg atgccgatga tgatgggaca
     1441 gggcttcatg gtgaccgacc aatgccagtc cgtatcgggc atctgtctct ggtacacatg
     1501 gcaggtcgtc agggacccct gcggcccgta gacctgttgc ggggcaagtg gcggcggcgc
     1561 tccgcgccgg ctgggtgccg ccgtcgcccg aaccaagacc cacgtcagtt ctgaggagat
     1621 cagtgatgca ttgctctgtg acgaccctca tagcgctctg ccttggcatg gcgcctgggc
     1681 tgcccttgtc tggaggaaca gaaccaccga gctggccata ctgggccgac tgggctctgc
     1741 ggccggtcgg gtccgacatg cgtgccggga gcatcgcggc cgcgttgggt gcgctctcgc
     1801 ggcaggctag agtgccgtgc atcgtgcccg tgcaggacga cactggggac ggcccaccca
     1861 catggctcag gcaagctgcc tctttcgaga cgacccctgg acacgagccg cgttccgccg
     1921 tatcggccat cagttactgg gcgcgcggaa acggccacga ggggttcacg acgccacact
     1981 acgtcgtgat ggtcccgtgt ccgcaacgac ttggccctga cagtctgccg ggggcctcct
     2041 acggcatcag agagagtgct ctggcgatcg ccattgccac cgcaccggcc gacgtggcgc
     2101 tccacctggc gtgcggcttc aaggtcatcg cgcagcagct cagcgaggag gcccaggcga
     2161 cactggtgcg cagcctgata gtgaaccgca gtggacacgg ctactccagg tcgctagaga
     2221 agccccttat gcttgccgtg agcctcgcgc ctacgctgat ctggcggccc agcc
//
LOCUS       DAOSPL010000796         2272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47524_length_2272_cov_3.505187, whole genome shotgun sequence.
ACCESSION   DAOSPL010000796 DAOSPL010000000
VERSION     DAOSPL010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47524_length_2272_cov_3.505187"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1664
                     /locus_tag="PLQ54_13795"
     CDS             <1..1664
                     /locus_tag="PLQ54_13795"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOS94387.1"
                     /translation="LSQLRPGLPGDIEIPTLSTSDGHTIYCVYSNNTQGIYIQATRDS
                     GLTWSDPVQIMEPGPRYTTDANILVDGDRLIVVATHVIDLPEQPGHLARSEFLACVSD
                     NGGQTWSEPQRIPVPRKYVSGCVNVPVWLEGDTVVMPYSWDVPAEEGNPAAEEGQMWC
                     KAGALISEDRGRTWTPGADIEVPVHPMGADEPAFVRLSNGDLFAIIRTGDRRPYEAVS
                     HDGGRTWADLKPSRFFGFNSPAALLRLRDGAIVRAWDDSPTDRFPLVVALSTDDCQTW
                     TPQRTVTEPTPNADGSLPFQSACYPSIAEAADGTILLAWWQRTTEGRNSVMLARTNRA
                     WIEEPAAWPKPQTIVAFGDSVTRGVRPGVTEYQTFRHLLQTKLREQGRNVRVVSAGVG
                     GHTTSDGLARIELDVLAQQPALTIVMFGLNDAAMVDGPEAARTAPRVSVEDYSANLRT
                     IVSRVREAGSKVLLCTPNPMTRKYDFQHVGAYAEHEDINFMLIHYVEATRQIAAEMGV
                     PLLDVYDLFMTRPGALDLLFDGVHPFVEGHQILADALLEKTEPML"
     gene            complement(1777..>2272)
                     /locus_tag="PLQ54_13800"
     CDS             complement(1777..>2272)
                     /locus_tag="PLQ54_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94388.1"
                     /translation="SAGLAPGPAGEAVPTPRPFAHSIAHCAVTAARLTREADAVRADL
                     LTAGGNAIETWVWRWADGRLSSATCTRVRGVVPFYDGVRDASTKGETKDWYKGVPVPR
                     MEQEFRFGAVVGMPDAVVPVSMTVVCEDEQFACDLRYFDYQVNMGLPEGCFGYDVPAA
                     GGAE"
BASE COUNT          372 a          871 c          665 g          364 t
ORIGIN      
        1 agctgtccca actacggcct ggcctgcccg gtgatattga gatccccacc ctcagtacta
       61 gtgatggcca cacaatctac tgcgtctact cgaacaacac ccagggcatc tacattcagg
      121 ccactcgcga ttccggcctc acatggtcgg atcccgtcca gatcatggag cccggccccc
      181 gctacaccac tgatgccaac attctggtcg atggagaccg tctcatcgtt gtcgccaccc
      241 acgtgatcga tctccccgag cagcccggcc acctggcccg cagcgagttc ctcgcctgtg
      301 tgagcgacaa tggcggccag acatggtccg agccacaacg cattcccgtg ccccgcaagt
      361 acgtcagcgg gtgcgtcaac gtgcccgtct ggcttgaggg cgacaccgtc gtcatgccct
      421 actcctggga cgtccccgcg gaggagggca acccggccgc cgaagaggga cagatgtggt
      481 gcaaggctgg cgcactcatc tccgaggatc gcggacgcac ctggacgccc ggtgccgaca
      541 tcgaggtgcc cgtgcacccc atgggggccg atgagcccgc cttcgttcgg ctctcaaacg
      601 gcgacctgtt cgccatcatc cgcaccggcg accggcgtcc ctatgaggcc gtcagccacg
      661 acggcggccg cacttgggcc gacttgaagc ccagccgctt cttcggcttc aactcacccg
      721 ccgcactgct ccggctgcgc gatggcgcca tcgtccgtgc gtgggatgac tcccccaccg
      781 accgcttccc gctcgttgtg gccctcagca ccgacgactg ccagacctgg acgccccagc
      841 gtaccgtcac tgaaccgact cccaacgccg acggctccct gcccttccag tcagcctgct
      901 atccctccat cgccgaggcc gcggacggca ccatcctcct cgcgtggtgg cagcggacga
      961 ccgaggggcg caactccgtg atgctagccc gcacgaaccg ggcatggatc gaggagcccg
     1021 ccgcctggcc gaagccgcag accatcgtgg ccttcggcga ctcggtgacc cgtggcgtcc
     1081 ggcccggcgt gaccgagtac cagaccttcc gccacctgct gcagacgaag ctccgtgaac
     1141 agggccgcaa tgtccgcgtg gtcagtgccg gcgtgggcgg gcacaccacc tccgacggcc
     1201 tggcgcggat cgagctggac gtgctcgccc agcaaccggc actcactatc gtcatgttcg
     1261 ggctcaacga cgccgccatg gtcgacgggc ccgaggccgc ccgcaccgcg ccgcgcgtct
     1321 cggtcgagga ctactcagcc aacttgcgca ccatcgtcag ccgtgtgcgg gaggccggct
     1381 cgaaggtgct gctctgcacg ccgaacccca tgacgcggaa gtacgacttc cagcacgtcg
     1441 gcgcctatgc cgagcacgaa gatatcaact tcatgctcat ccactacgtc gaagccacgc
     1501 gccagatcgc ggccgagatg ggcgtgcccc tcctcgatgt ctacgacctt ttcatgacgc
     1561 gacccggcgc gctcgacctg ctcttcgacg gcgtgcaccc cttcgtcgag ggccaccaga
     1621 tccttgctga tgcactgctc gagaaaaccg agccgatgct gtagggccgg ctgcccggac
     1681 aggaagacga gtcccgaccc ctcttccctt tggcgttgcc gttgtagggg cgctgcttgc
     1741 tgcgcccggg cctgttcctg ccggccacac ccgcccctac tccgccccgc ctgccgccgg
     1801 cacatcgtac ccgaagcatc cctccggcag ccccatgttg acctggtagt cgaagtaccg
     1861 caggtcacac gcgaactgct catcctcgca gaccacggtc atcgatacgg ggacgacagc
     1921 atcgggcatg ccaaccactg cgccgaaccg gaactcctgc tccatccgcg gcaccggcac
     1981 gcccttgtac cagtccttcg tttcgccctt cgtgctcgcg tccctgaccc cgtcgtagaa
     2041 cggcacgacg ccacgcaccc gggtgcacgt ggcgcttgag agccggccgt cggcccatcg
     2101 ccagacccac gtttcgatgg cgttcccgcc cgcggtcaag aggtcggccc gcaccgcatc
     2161 ggcctcacgc gtcagccgag ccgccgtcac cgcgcagtgc gcgatgctgt gcgcgaacgg
     2221 cctcggcgtt ggcaccgcct cgcccgccgg tccgggagcc agcccggcag ag
//
LOCUS       DAOSPL010000797         2268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47643_length_2268_cov_3.441482, whole genome shotgun sequence.
ACCESSION   DAOSPL010000797 DAOSPL010000000
VERSION     DAOSPL010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47643_length_2268_cov_3.441482"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..2110)
                     /locus_tag="PLQ54_13805"
     CDS             complement(116..2110)
                     /locus_tag="PLQ54_13805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOS94389.1"
                     /translation="MLRFASEVCADLHELTRREWLVANGIGGYASSTLCFLNTRRYHG
                     MLVAALQPPVARTVLVAKCEETVRTSAGVAELGCNAYPKVVYPDGYEYLQDVRLELGV
                     PSFSYRVLGGTLLKTVAMVQGQNTTLVRYMWDGPAGAELLVHPLITCRDYHWLKRFDP
                     WLSREWAPHAGGAWYQPYELGPRIYVSATEGTYCHDPVWYYNFQYEQERQRGLEFEED
                     LYRPGELALPLASGEPAYLVLSTEPRRAEEAPALFDAEMARRHAVLRRCPMAGHRAEV
                     DELILAADSFLVRRGSGLGSILAGYHWFGDWGRDTMIALPGLTLTTGRAEDARQILLT
                     YAEFVDQGMLPNRFPDSGEAPDYNTVDATLWYFVALHYYVEATGDLDTARRLLPVLME
                     IIRAHVRGTRYGIGVDSDGLLAAGDERTQLTWMDAKVDDWVVTPRNGKPVEINALWYN
                     ALRITADLAGRLAMPAQAREAAEHAERAAASFVPAFHDAAAGYLADRVVDGVRDQTLR
                     PNQLLAASLPYKLIDAERTRQMLDRIDRGLLTPHGLRTLAPSEPGYRGIYLGDRWARD
                     GAYHQGTAWAWLIGPYVDAWLYAHAGEPGAERAALGLLDPLRRQVYDAGVGSLSEIYD
                     GAAPHEPRGCISQAWSVGEALRILGRLLPANAPAGRPSGS"
BASE COUNT          386 a          798 c          738 g          346 t
ORIGIN      
        1 aggagcacta ccacaccggc catgcgccgg gcgtgtacca taagaggacc tcacttagcc
       61 gtcagcgggg cccacgccgg gcccccagga gtccactact cgcctttaga cacacctatg
      121 agcccgaagg tcgcccggcg ggcgcattcg ccgggagcag gcggccgagg atgcgcagcg
      181 cctctccgac gctccacgcc tgcgagatgc atcctcgtgg ctcgtgcggt gctgcgccat
      241 cgtagatctc cgacaggcta cccactcccg cgtcgtagac ctgccggcgc agcggatcga
      301 gaagcccgag agccgcccgc tcggcgccgg gctcgcccgc gtgcgcatac agccacgcgt
      361 ccacgtacgg gccgatcagc cacgcccacg cggtgccctg atgataggcg ccatcgcgtg
      421 cccatcggtc accgaggtag ataccgcggt agcccggctc actgggggcg agcgtccgca
      481 ggccatgggg cgtcaacaac ccgcgatcga tccggtccag catctggcgt gtccgttcgg
      541 catcgatcag cttgtacggc agactcgcgg ccaacagctg gttcggccga agagtctgat
      601 cgcgcacgcc atccacgacg cgatcggcga ggtacccggc ggccgcgtcg tggaatgccg
      661 gcacgaagga agccgcagcg cgctccgcgt gctcggccgc ctcccgcgcc tgggccggca
      721 tggcgagtcg gccagccagg tcggccgtga tgcgaagggc gttgtaccag agcgcgttga
      781 tctcgaccgg ctttccattc cgtggggtga ccacccagtc atcgaccttg gcgtccatcc
      841 acgtcagctg tgtccgctcg tcgccagcgg cgagcagccc gtctgagtcc acgccgatgc
      901 cgtaccgcgt accccgcaca tgggcgcgga tgatctccat gagcaccgga agcagccgcc
      961 tggcggtgtc gaggtcgccc gtagcttcga catagtagtg gagagccaca aagtaccaca
     1021 gagtcgcgtc caccgtgttg tagtcgggcg cctcacccga gtcggggaac cggttgggca
     1081 gcatgccctg atccacgaac tcggcatacg tgagcaggat ctgccgggcg tcctcggcac
     1141 ggcccgtggt cagggtcagg ccgggtaggg cgatcatcgt gtcccggccc cagtcaccga
     1201 accagtggta gccagcgaga atggacccga gcccgctgcc ccgccggacg aggaagctgt
     1261 ctgccgccag gatcagctca tccacctcgg cacgatgccc ggccatgggg caccggcgca
     1321 gcacagcgtg gcggcgcgcc atctcggcgt cgaacagggc cggggcctcc tcggcccggc
     1381 gcggctcggt tgagaggacc agatacgcgg gttcgcccga tgccagtggc agcgccagct
     1441 cgcccggccg gtacaggtct tcctcgaact ccaggccgcg ctgtcgctcc tgttcatact
     1501 ggaagttgta gtaccatacc ggatcgtggc agtacgttcc ctcggtggct gaaacataga
     1561 tgcggggccc cagctcgtag ggctggtacc acgcaccgcc cgcatgagga gcccattcgc
     1621 gtgacagcca cgggtcaaat cgcttcagcc agtggtagtc gcggcaggtg atcagtgggt
     1681 gcacgagaag ctcggcccct gctggcccat cccacatgta gcgcaccagg gtggtgttct
     1741 gtccctgcac catggccacg gtcttgagca gcgtgccgcc caggacccga tagctgaacg
     1801 aagggacgcc gagttccagc cgcacgtcct ggagatactc gtacccatcc gggtagacca
     1861 ccttggggta cgcattgcac ccgagctccg caacgcctgc ggatgtccgc acggtctcct
     1921 cgcacttggc gaccagcacg gtgcgggcca cgggcggctg cagtgcggcc accagcatgc
     1981 cgtgatagcg ccgtgtgttc aggaaacaca gggtggatga ggcataccca ccgataccgt
     2041 tagcgaccag ccattcgcgc cgggtcaact cgtgcaggtc ggcgcagacc tcagaggcga
     2101 agcgcagcaa ggccgtccac tcccttgggg gcgagttggg ccccggcctc cgaaccacgc
     2161 gccagggcaa ccctctaccc taacggccgc agcgcctcga acgctcacct gtctcgcaag
     2221 ccggctggac tgaggccgag cctaatcctc ttcggcccgg ggccgcat
//
LOCUS       DAOSPL010000798         2268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47649_length_2268_cov_3.151378, whole genome shotgun sequence.
ACCESSION   DAOSPL010000798 DAOSPL010000000
VERSION     DAOSPL010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47649_length_2268_cov_3.151378"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLQ54_13810"
     CDS             <1..192
                     /locus_tag="PLQ54_13810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94390.1"
                     /translation="HDNMGGAWCNMINNAPNTLERYTRHNGGSNIGFMDGHAKFRTAL
                     QCKQYPWGDIVVDNGQQPT"
     gene            279..500
                     /locus_tag="PLQ54_13815"
     CDS             279..500
                     /locus_tag="PLQ54_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94391.1"
                     /translation="MRVAVLLCCVVLVLSLVTASVVGCKGGNASTDPNAKEASRQKAL
                     KQQDEMKNMMNNMKKQGVSQPNEGAAQGG"
     gene            1059..1607
                     /locus_tag="PLQ54_13820"
     CDS             1059..1607
                     /locus_tag="PLQ54_13820"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS94392.1"
                     /translation="MKSTVQRARAGDPEAREAVVRCLTSRVNSCSRYYSRRCGVDVDD
                     LRQEMWLGIFEALQRVDVSIGDPVQYLLSHGRFALLTSLRRRQKDNPGPDEAAEQVPV
                     DGSLERSVIDGQVVQTFVSSLDTTAHRIVSCLLQGHSRSETAKLLGCTPANITYHLRR
                     VESSLSALLAAESRAAYAGGAR"
     gene            complement(1677..>2268)
                     /locus_tag="PLQ54_13825"
     CDS             complement(1677..>2268)
                     /locus_tag="PLQ54_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94393.1"
                     /translation="MPTAGTVIVRLGTVSNRARLQIRLDGELKVDRELLTGEMGEGPW
                     KEARWLSEYGIWNCRYDEDIPIEVPEGEHTLSITNAEGDWLQIPWIRLPAYRSSRFPD
                     VNAIGLSSDRLILLWLHNRQSTWRTEFEGRAPAVLTSLRVTVPVPADGAWRVEWWNTF
                     NAEVTQRETLAAAGGQLVLRAPDLMRDVAVRMERAE"
BASE COUNT          395 a          774 c          743 g          356 t
ORIGIN      
        1 cacgacaaca tgggcggtgc ctggtgcaat atgattaata atgcaccgaa caccctggag
       61 cggtacaccc ggcacaacgg cggatcgaac atcgggttca tggacggaca tgccaagttc
      121 cgcaccgccc tgcaatgcaa gcagtatccg tggggcgata tcgtcgtgga caacggccaa
      181 cagccaacgt agagcccgtg cgcgggccgt cgtggcatcg aatgggcgga gcttcgtggg
      241 aagtctccgc cccacttcgg aaggcaggtc ttgccggaat gcgtgtggca gtgttgctgt
      301 gctgtgtggt gctggttctc tccctggtca cggcgagcgt ggtgggctgc aaagggggca
      361 atgcatccac cgacccgaac gccaaagagg ccagccggca gaaggccctc aagcaacagg
      421 acgagatgaa gaacatgatg aacaacatga aaaagcaggg cgtctcccag cccaacgagg
      481 gagccgccca gggcgggtag cgtaccgcct ggtggacatc cggacgactg cccggcgccc
      541 cttcgggcgg gtgccgggca gttgtgccgt gtggggacag aaaagtgtcg aaaacccctc
      601 aaacgatccc ggtcggtgcg cgatatactc ctgtgaacgg tacggctccc ctgaggggag
      661 cgcaactcga taccgctggt ggggaccgct cggatgcccc acctactagc gctggttcgg
      721 cactggagtg atctggaaca tgcgcgacgt tcccggccag cgcggaaagg acaccagggc
      781 ccatgactac tgcgatcgat cgcactatgg ccgacggcac gcgaatgccg ttgaggctcg
      841 accgactacc ccgcatgtgc tctagcaggc ggcttcatcg caaggctccg ctagacgcgg
      901 gtagttcgaa tctcatcgcg gaccggcgcc cgggtgtccc acaagccctt ctcggtcccc
      961 cagcggagta gccgcgggga cccccctccg ggtcactcgg aaccaccggg ccgcagacca
     1021 gacgacaggc ccaggttcga gagagtgagt gacccacgat gaagtccacg gtacagcgtg
     1081 cccgagccgg cgatcccgaa gcccgcgaag cagtggtgcg gtgcctgacg tcccgggtga
     1141 actcctgcag ccgatactac tcacgccgct gcggcgttga tgtcgacgac ctgcgccaag
     1201 agatgtggct cggcatcttc gaggccctgc agcgtgtcga tgtatcgatc ggcgaccccg
     1261 tccagtacct gttgtcacac ggccggttcg cgctcctgac ctccctgcgc cggcgccaga
     1321 aggacaaccc ggggcctgat gaggcggccg aacaggtgcc ggtggatggg tccctcgagc
     1381 ggtccgtcat cgacggccag gtcgtgcaga ccttcgtcag ctcgctggac acgacggccc
     1441 atcgcatcgt gtcgtgcctg ctccaggggc actcgcggtc cgagacggcc aagctgctgg
     1501 ggtgcacgcc ggctaacatc acctaccacc tgcggcgagt cgagtcgagc ctgagcgctc
     1561 tcctggccgc ggaaagccga gccgcctacg ccggcggagc ccgctaactt cgcacgccgg
     1621 gggtcgcgcg ccgtgcggcc cccggccggg cccgcggggc gccctggggg ggacccctat
     1681 tccgcccgct ccatccgcac ggccacgtcg cgcatcaggt ccggtgcgcg gagcacgagc
     1741 tgacccccgg cagccgccag cgtctcgcgt tgagtcacct cggcgttgaa cgtgttccac
     1801 cactccaccc gccacgcgcc atcggcggga acgggcaccg tcacgcgcag ggacgtgagc
     1861 accgccggcg ccctgccctc gaactcggta cgccacgtgc tctgtcggtt gtggagccac
     1921 agcaggatca gacggtcgct cgacagtccg atggcgttca cgtccgggaa ccggctgctg
     1981 cggtaggcgg ggagacggat ccaggggatc tggagccagt ccccctcggc gttggtgatg
     2041 ctcagcgtgt gttcgccctc cggcacctcg atcgggatgt cctcatcgta ccggcagttc
     2101 cagatcccgt actcgctgag ccacctggcc tccttccacg gtccctcgcc catctcgccc
     2161 gtgagcagct cccggtcgac cttcagctcg ccatcgagcc ggatctgcag ccgggcccgg
     2221 ttcgagaccg ttccgagccg gacgatcacc gtgcccgccg tgggcatg
//
LOCUS       DAOSPL010000799         2267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47668_length_2267_cov_4.283454, whole genome shotgun sequence.
ACCESSION   DAOSPL010000799 DAOSPL010000000
VERSION     DAOSPL010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47668_length_2267_cov_4.283454"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PLQ54_13830"
     CDS             <1..546
                     /locus_tag="PLQ54_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOS94394.1"
                     /translation="GLWGEGAFLGWEIWQNNDLSWHYWYVLEVGESDISHVIFQVSDT
                     FTGDDIVGGSIKLNDEPGFYDEIGTQKTGQGNVGMPSGIWGIKFSGTSTSMTYAFDSW
                     RVPVWQDFYAKGGRTGGDWNYLYNAGFTDPNPLVGAHDGTEGYHILAPDTRTTQTPEP
                     SVCALALAGLGGIWARKRRRR"
     gene            700..>2267
                     /locus_tag="PLQ54_13835"
     CDS             700..>2267
                     /locus_tag="PLQ54_13835"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOS94395.1"
                     /translation="MEGRSIAYETYKDKVLGCWLGKFIGGTIGAPAEGHKTRMDFTEE
                     NCWPSEIVPNDDLDIQVCWLEVLEDCGPFIDAEDLIQGWQERCWYNFAEYGCFLYNVQ
                     RGIMPPLSGTYNNTFYRESMGCPIRAEIWGAVSPGNPALAAEYALLDGQLDHTGDSVY
                     AEQFLAGCTAEAFVCDDLREVIAAGAAFVPPDSGMGRVIGEIPVICGRHSTWQGARRE
                     IIRRWGHRDCTSVLVNQAFVLMALHLARTDFLAAVLMAANCGWDTDCTAATVGALVGT
                     ILGAKALPKKLVSKVGGRLNCDIAVRHRNALLRDFAEDTCRVGVEVTAARSPGIRIEG
                     APALSIRGPRRAAIEVAASHPDEPVLTRDGQAVVDLRLSNHSIYRVNGTLSIETPEGL
                     MASPDSVPITLPTRESGRIRVSITTSRDAPVLWDRNAFRATFRPQRGADEWSCRFGLV
                     GARQWEVYGPYFDIYDTRLHDECPFHNEHVMKNPSLVAGCGGALCHNYVRLDRAYLDE
                     AALAAGPLADEEPFL"
BASE COUNT          388 a          719 c          772 g          388 t
ORIGIN      
        1 ggtctgtggg gcgagggggc gtttctcggc tgggagatct ggcaaaacaa tgatctctcg
       61 tggcactact ggtatgtcct cgaagtcggg gagagcgata ttagccacgt gatcttccag
      121 gtatccgaca cgttcacggg cgatgatatc gtcggtggat ccatcaagct gaatgacgaa
      181 ccgggcttct acgacgagat cggcactcag aagacgggcc agggcaatgt cggcatgccc
      241 tcaggtatct ggggcatcaa gttctcaggc acgtccacca gcatgaccta cgcgttcgac
      301 tcgtggcgcg ttccggtctg gcaggacttc tatgccaagg gcggcagaac cggcggcgac
      361 tggaactacc tctacaacgc cgggttcacc gacccgaacc cgctggttgg cgcccacgac
      421 ggcacggagg ggtaccacat tctggccccg gacacacgca cgacgcagac accggagcca
      481 tcggtctgtg ccttggcact ggcagggctg ggaggcatct gggcccgcaa gcgccgtcgc
      541 cggtagactc agctgtcaag ccgattgtgc cgtgaacggg ccgctccccc gtgtgggagc
      601 ggcccgtggc gcgtgtgtgg cgttgtggtg gtgcgccaag aggaccagct ggctcccctc
      661 ccgaagctcc caggaccgac gagggggagt cgctgaccca tggaagggcg tagcattgcc
      721 tacgagacct acaaggacaa agtccttggc tgctggcttg gcaagttcat cgggggaacg
      781 atcggcgctc ctgccgaagg gcacaagacc cgtatggact tcaccgagga gaactgctgg
      841 ccctcggaga tcgtcccgaa cgatgacctc gacatccagg tttgttggct cgaggtgctc
      901 gaggactgcg gcccgttcat cgatgcggaa gacctcatcc agggctggca ggagcgttgc
      961 tggtacaact tcgccgagta tggatgcttc ctctacaacg tgcagcgggg catcatgcct
     1021 ccgctgtcgg ggacctacaa caacacgttc taccgcgagt ccatggggtg ccccatccgc
     1081 gccgagatct ggggcgcggt atcgccgggc aatcccgctc tggcggccga gtatgccctg
     1141 ctggatggcc aactagacca cacgggcgac tcggtctatg ccgagcagtt cctcgcgggt
     1201 tgcacggctg aggcgttcgt gtgcgacgac ctccgcgagg tcatcgctgc gggtgctgcg
     1261 ttcgtcccac ctgattcggg catgggtcgc gtcattggcg agatcccggt catctgcggg
     1321 agacactcga catggcaggg ggcgcggcgg gagatcatcc ggcggtgggg gcaccgcgac
     1381 tgcacgtcgg tactggtgaa ccaggctttc gtgctcatgg ccctgcatct ggcacggacg
     1441 gacttcctcg ccgccgtgct gatggccgcc aactgcggat gggacacgga ctgcaccgcc
     1501 gccaccgtgg gggcgcttgt cggcaccatc ctcggcgcca aggcgctgcc gaagaagctg
     1561 gtgtcgaagg tgggaggccg gctcaactgc gacatcgcgg tgcgccaccg caacgcgctg
     1621 ctgcgcgact tcgccgagga cacgtgccgc gtaggggtcg aggtcacggc ggcccgctcg
     1681 ccgggtatcc gcatcgaggg cgcgcccgca ctctcgatcc gaggaccgcg gagggcggcc
     1741 atcgaggtcg cggcgtcgca cccggacgag cccgtgctca cgcgtgacgg ccaagccgtg
     1801 gtcgatctgc ggctctcgaa ccacagcatc taccgcgtga atggaacgct ctcgatcgag
     1861 acacccgagg ggctcatggc gtcgcccgac tccgtgccca tcactctgcc gacgcgcgag
     1921 agcggacgca tccgcgtgtc gatcaccaca tcgagggacg cgccagtgct ctgggaccgg
     1981 aacgccttcc gggccacatt ccggccgcag cgaggggccg atgagtggtc atgccggttt
     2041 gggctcgtgg gggctcgcca atgggaggtc tacgggccct acttcgacat ctacgacacg
     2101 cgtctccacg atgagtgccc gttccataac gagcatgtca tgaagaaccc gtcgctggtc
     2161 gcggggtgcg gcggcgcgct gtgccacaac tacgtccggc tcgaccgagc gtacctggat
     2221 gaggccgcgc tggcggccgg gccgctggcc gatgaggagc cattcct
//
LOCUS       DAOSPL010000800         2262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47813_length_2262_cov_3.829180, whole genome shotgun sequence.
ACCESSION   DAOSPL010000800 DAOSPL010000000
VERSION     DAOSPL010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47813_length_2262_cov_3.829180"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2262
                     /locus_tag="PLQ54_13840"
     CDS             <1..>2262
                     /locus_tag="PLQ54_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOS94396.1"
                     /translation="RGNGVRVFRPSLTATLAARETSVLDGLRALDGGGLEPQQAGEPG
                     AAVFKVEGANVITSLTIAGSFRRATEADANSVAISITNGLTWTDVWTNAGTGEVPLSL
                     ELVELVNGAYEVLVRVSLTAATAPANAQLLGIEFRTITQVNAKAQPRLTLGRNTVYVG
                     AGAQEGSVVFWPDLRGDRYRPWVIEERNIATEAEHPGYQGVMHAARPGEDAYVVFRLD
                     VPGDMTRVTYGGRLYNRAPGSRIAFLHSFDGGATWSESYALTDTEQPWDVIHFVAAEA
                     PARVRSVRVKYLMQSPAAGKDACSLYSVRMEANYAPSDAGPTPLEVTFGWAERQADYS
                     LVERTHTQLVTQLPARYIVNVGGADHPIMRFLRVQPRGLEPAAYGYSDGIDAGGERHV
                     PRWVTYGRNLAVGKPYTVSVPSNDDWGAGDPEGKRLTDGIVGPYYAGGVTPTYCLGWN
                     DGQVVDITIDLGERQHCGGFRIHLGGGWPWWDALKGEVRDTVEVLTSTDGEQYISRGP
                     FDLNPWRKDLPINLMLPDEETLGAYLYDLALPEPVDARFVRFRLTARRSVCVSEVQVL
                     DEIRSEPFDLRIALPDERPPGSGEVPKVLTPTGRPIPAARAQANSAEPLGEPVLEPAT
                     LRSLGVHWIIAGDENENARVTLAYRRHGDHQWQEGLPLFRVERGAHRNEKGESSVEVS
                     DDAWLFAGSALLLEPDTAYELRLRLTDPDGGGAERVLQARTITEPLAPNGMRTRYVTP
                     GHGGGTGSQGDPFR"
BASE COUNT          361 a          781 c          765 g          355 t
ORIGIN      
        1 ccgcggcaat ggcgtgcgcg tcttccgccc ctcgctcaca gccacgttgg ccgcccgaga
       61 gaccagcgtg ctcgatggcc tgcgggccct tgatggcggc gggctggagc cccagcaggc
      121 cggcgaaccg ggcgcagcgg tgttcaaggt cgagggcgcg aacgtgatca ccagtctgac
      181 catagctggg agcttccgcc gggccactga agccgacgcc aactccgtcg ccatcagcat
      241 tacgaacggg ctgacgtgga ccgacgtgtg gaccaacgcg ggtacgggcg aggtgcctct
      301 ctcactcgag ctcgtcgagc tggtcaatgg cgcctacgag gtgctcgttc gagtctcgct
      361 gaccgctgcc acggcgccag ccaatgcaca acttcttggt atcgagttcc gcacgatcac
      421 tcaggtgaac gccaaggctc agccccgact gacgctcggg cggaacaccg tgtacgtcgg
      481 cgcgggtgcc caggagggct cggtggtatt ctggcccgac ctccggggcg atcgctacag
      541 gccctgggtc atcgaggaac gtaacatcgc caccgaggcc gagcaccccg ggtatcaggg
      601 cgtcatgcac gccgcgcgtc cgggcgagga cgcatatgtg gtgttccggc tcgatgttcc
      661 cggcgacatg acgcgggtca cctatggcgg ccggctgtac aatcgcgccc caggctcccg
      721 gatcgccttc ctgcactcct tcgacggcgg cgccacttgg agcgagagct acgcgctcac
      781 ggacacggag cagccatggg atgtcatcca cttcgtggcc gccgaagcac ccgcgcgggt
      841 gcgttcggtg cgggtgaagt acctcatgca gagcccggcg gccggcaagg acgcttgtag
      901 cctctactcg gttcgcatgg aggccaacta tgctccgtcg gacgccggcc cgacgccgct
      961 ggaagtcacc ttcggctggg ccgagcgcca ggccgactac tcgctggtcg agcggaccca
     1021 cacccagctc gtgacgcagt tgccggcacg gtacatcgtc aacgtcggcg gggccgatca
     1081 cccgatcatg cggttcctgc gggttcagcc ccggggcctc gagccggcag cttacggcta
     1141 ctccgatggc atcgatgccg gtggcgaacg gcacgtcccg cgctgggtta cctatggccg
     1201 caacctggcc gtcggtaagc cctacacggt ttcggtgccc tccaacgatg actggggcgc
     1261 cggcgatccc gaaggcaagc gcctgaccga tggaatcgtc gggccctact atgccggggg
     1321 cgtgaccccc acgtactgcc tcgggtggaa cgatggccag gtcgtcgaca tcaccatcga
     1381 tctgggtgag cggcagcact gcggcggttt ccgcatccat ctgggtggcg gatggccttg
     1441 gtgggatgcc ctgaagggcg aggtccggga cacggtcgag gtgctcacat cgacggatgg
     1501 cgagcagtac atcagccgag gcccattcga cctcaacccg tggcgaaagg acctacccat
     1561 caacctgatg cttcccgacg aggagacgct cggggcctac ctgtacgacc ttgccctgcc
     1621 cgagccggtg gacgcgcgct tcgttcgctt ccgcctgacg gccaggcgct ccgtctgcgt
     1681 cagcgaggtt caggtcctcg acgagatccg gtcggagccc ttcgacctgc gcatcgccct
     1741 gcccgatgag cggccgcctg gcagcggcga ggtgccgaag gtattgacgc ccaccggccg
     1801 gcccattccc gccgcgcggg cgcaggccaa ctcggccgag cccctgggcg aacccgtgct
     1861 tgagcccgcg acccttcgct ctctcggcgt ccactggatc atcgccggcg atgagaacga
     1921 gaacgcgcgg gtgacgctgg cctaccgtcg ccacggggac catcagtggc aggagggcct
     1981 cccgctgttc cgcgtcgaga gaggcgctca ccgtaacgag aagggcgaga gcagtgtcga
     2041 ggtgtccgac gacgcgtggc tgttcgcggg cagcgccttg ctgctcgagc ccgacacggc
     2101 ctacgagctc cggctccggc tcactgatcc cgacggcggc ggggccgaga gggtcctgca
     2161 agcccgcacc atcacggagc cgctggcacc taatgggatg cgcacgcgct atgtcacccc
     2221 gggccatggc ggcggcaccg gcagccaggg cgacccgttc cg
//
LOCUS       DAOSPL010000801         2260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47875_length_2260_cov_4.242177, whole genome shotgun sequence.
ACCESSION   DAOSPL010000801 DAOSPL010000000
VERSION     DAOSPL010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47875_length_2260_cov_4.242177"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..907)
                     /locus_tag="PLQ54_13845"
     CDS             complement(44..907)
                     /locus_tag="PLQ54_13845"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOS94397.1"
                     /translation="MLTGYSKALYASWFYYKPARLLLDAGEGIVHALGKRIFGIRKVF
                     ISHGHEDHIAGLPSLVNLRNLSMGDRQVPLRIYHPQADPFVGFLQDYLDKKQRDRLRY
                     SMEWIALAPGETVEIETARRSTRARAFPVNHTRGWQSLGYRLEERRQRLKAEFAGLET
                     HEVRELVREQGRESVVDVVWHPLVVYTGDARPAEDIEIFRQADLLLIDATFRNNEEAA
                     GSRHGHIDASIALGIQAQVKHLVLMHLSERYTLRDIRKAIRVAGSMYGYPPQLGFFFE
                     GRYCEMERKEE"
     gene            1527..>2260
                     /locus_tag="PLQ54_13850"
     CDS             1527..>2260
                     /locus_tag="PLQ54_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94398.1"
                     /translation="MNRVFVTFAVQMFAAWVGLLGVAFYWATQTACGGGAAVLADSGV
                     DISGVQTGRQIDRGGRASNALANPRLQHPKVDMLKSEIQEFRNLKNVSTSYKNAYNGL
                     LNYANQWLAHTTSYPFLQKAAWERELRYMRYAYLATGDAKYAEHAKALALYAAENWVP
                     QPGISSIDNWTGIRGCREATAAFARTYDTLYDYLTAAERATLLDATRQWGTILLADMK
                     KYFYNQDPEGVLYGPHIEKGISYLAQA"
BASE COUNT          422 a          719 c          719 g          400 t
ORIGIN      
        1 agccttctcc acctgactcc gtctcctcgg caggttcccc gccctactcc tctttccgct
       61 ccatctcgca gtagcgaccc tcgaagaaga acccgagctg aggcggatag ccatacatgc
      121 tcccggcgac gcggatggcc ttgcggatat cgcggagcgt gtagcgttcg gagaggtgca
      181 tgagcaccag gtgtttcacc tgagcctgta tgcccagggc gatgctggca tcgatgtggc
      241 cgtggcggct ccccgccgct tcctcgttgt tgcggaaggt ggcgtcaatc aggagtagat
      301 cggcctggcg gaagatctcg atgtcttcgg ctggccgggc atctcccgtg tagaccacca
      361 gcggatgcca caccacatcg accacggatt cgcggccctg ctcgcgcacc agctcccgta
      421 cctcgtgggt ctcgagcccc gcgaactcgg ccttgaggcg ctggcgccgc tcctccagtc
      481 gataacccag gctctgccag ccgcgggtgt ggttcaccgg gaaggcccgg gcgcgggtgg
      541 agcgccgggc ggtctcgatc tcgaccgtct cgcccggagc cagggcgatc cattccatgc
      601 tgtaccgcag gcggtcgcgt tgcttcttgt ccagatagtc ctggaggaac cccacgaagg
      661 ggtcggcctg ggggtggtag atccgcaggg gtacctggcg gtcgcccatg ctcagattcc
      721 gcagattaac cagactcggc agaccggcga tgtggtcctc atgcccgtgg gagatgaaga
      781 ccttgcggat gccgaagatg cgcttgccta aggcgtgcac gatgccctcg cccgcgtcca
      841 gcagtagccg ggcgggtttg tagtagaacc aggaagcata gagggccttg gagtagccgg
      901 tcagcacatc ctccaaccca agcccacctc cgcagggcta ccatagcgtg ccgcgtgggc
      961 ggtgtgcaag gaagagcgga ccggcgagag ccctgcgcag ccctctggaa gccaagcgcc
     1021 ctcgccttac cagaagtggt gtagcccaac tcacactaag cttcccaccc cgtcagcagt
     1081 tggctctggc tgccagagac agggtgccgt acggccccgt tgaaccccct ggaacgcgcc
     1141 ggacactcgg gccgttcggg tgcgtccatt ccccgatttg aggcgcgaca tagcctccgg
     1201 ccgcgttgta gcgcgccctc gcgctgtgga acactgactc cagtagttga gagtgcggcg
     1261 tactgcgtct cagagggctg ccagagcggt atgccgagag gaggctgcca gctatgtcaa
     1321 atgtgaatga gccatcagat ctgcacggtg gatctggtgg cctctcgcga tcgccatcgc
     1381 ggtccgaccc gccaggcact caccattcgg tggacttgcg ggtcacaacg cctcgatggc
     1441 tcggcgggtc gtacatcgat ctgagaatgg ggagggaccg gagagcggca aagcggccac
     1501 acacggtacc gaccagagtt cgacggatga accgggtgtt cgtcaccttc gcggttcaga
     1561 tgttcgcagc gtgggtgggt cttctcggtg tggccttcta ctgggctaca cagacagcgt
     1621 gcggcggggg agccgcagtc ctggccgatt caggtgtgga tatcagcggc gtgcagacgg
     1681 gacgccaaat cgaccggggc ggccgcgctt ccaatgccct ggccaatccg aggcttcagc
     1741 atccgaaggt cgacatgctg aagtccgaga tccaggagtt caggaacctc aagaacgtga
     1801 gtacttcgta taagaatgcg tacaatggcc tcctcaacta cgccaaccag tggctcgcgc
     1861 atacgacgag ctatccgttc ctgcagaaag cagcatggga acgcgaactg cggtacatgc
     1921 gctatgccta cctggccacg ggtgacgcca aatacgccga gcatgccaaa gccctggccc
     1981 tgtatgccgc ggagaactgg gtgccgcagc caggtatcag cagcatcgac aactggacgg
     2041 gcatccgcgg gtgccgcgag gccacggcag ccttcgcgcg cacgtacgac acgctctacg
     2101 actatctgac tgccgcggag agagcgacgc tcctcgatgc gactcgacag tggggcacca
     2161 tcctgctggc ggacatgaag aagtacttct acaaccagga ccctgaaggt gtcctctacg
     2221 ggccgcacat cgagaagggc atctcctatc tcgcccaggc
//
LOCUS       DAOSPL010000802         2260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47880_length_2260_cov_3.000907, whole genome shotgun sequence.
ACCESSION   DAOSPL010000802 DAOSPL010000000
VERSION     DAOSPL010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47880_length_2260_cov_3.000907"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1846
                     /locus_tag="PLQ54_13855"
     CDS             <1..1846
                     /locus_tag="PLQ54_13855"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOS94399.1"
                     /translation="DQTSLSCEMRLREFRGHPAFDWVVRVRNEGTADSAKIHDFWGID
                     TCWNATDGSMPILHRSIGSPGHEDDFQFRSEFMHNSMWDKRRRIPMDTPSNTRWGEIH
                     SYNIPNDHRSSAVWLPFFNYQTGGDGLITGLGWSGAWRAYFDHQGGGSSTILAGVENL
                     DTILRPGEAVRSTLNLALYWQGELMHGQNLFRRLVLDHYTPHMDGKPVEPPIPGVVWG
                     GIPSAEHLEIIEKIQEYEIPFDVYWFDAGWYGTGTVPSYSVFEGDWGIMAGDWRPNPN
                     WHHGTLKPVSDAAHALGMKFLVWVEPCRALQGRPVTLEHPEYFLTGNADGEARDGQTL
                     LLDLGNPEALNWAVDVVASLVEDYGMDWYEEDFNLDPNPYLEAAAEPDRWGMREMRFI
                     EGHFAFWDTLLQRFPNLAILNCASGGRRIDLESISRGEPLWRSDYNCFPEATAESTQD
                     QSYGIQHWLPIQGSMIRGWSMYDTYEARCALSPGMETGIVAKTPEEWLQLKANVIQAK
                     RCKRYFMGDYYPLANQQRDPAAWTAYHLFLPEEQEGMLVALRRAKSDVAAMTFDLLTI
                     DPARQWEFEDYDSGKRWVASGRQIREEGFEVAITQRRGSRLIFYRPAP"
BASE COUNT          390 a          741 c          792 g          337 t
ORIGIN      
        1 cgatcagacc tcgctctcgt gcgagatgag gttgagggag ttccggggcc atccggcctt
       61 cgactgggtc gtgcgggttc ggaacgaggg gaccgccgac tcggcgaaga tccacgactt
      121 ctggggtatc gacacgtgct ggaacgccac agacggctcg atgcccatcc tgcaccgctc
      181 gatcggctcg ccggggcacg aggatgactt ccagttccgc agtgagttca tgcacaactc
      241 gatgtgggac aagcggcgca ggatcccgat ggacacgccg tcgaacaccc ggtggggcga
      301 gatccactcg tacaacatcc cgaacgacca tcgctcgagc gcggtctggc taccgttctt
      361 caactaccag acgggcggcg acggcctcat cacggggctc gggtggagtg gtgcctggcg
      421 agcgtacttc gatcaccagg ggggcggatc gtcgaccatc ctggccggcg tggagaacct
      481 cgacacgatc ctccgtcccg gcgaggcggt gcggtcgacc ctgaacctgg cgctgtactg
      541 gcaaggcgag ctgatgcacg ggcagaacct gttccgccgg ctcgtgctgg accactacac
      601 gccgcacatg gacggaaagc cggtggagcc accgatcccc ggggtcgtct ggggcggtat
      661 cccgtcggcc gagcacctgg agatcatcga gaagatccag gagtacgaga tccccttcga
      721 cgtctactgg ttcgacgccg gttggtatgg gacggggacc gtcccctcat acagcgtctt
      781 cgagggcgat tggggcatca tggccggaga ctggcggccg aaccccaact ggcaccacgg
      841 cacgctcaag ccggtcagtg atgcggcgca cgctctgggg atgaagttcc tcgtctgggt
      901 cgagccatgc cgggcgctgc agggccggcc ggtcactctc gagcacccag agtacttcct
      961 caccgggaac gccgacggtg aggcgcgcga cgggcagacc ctcctcctgg acctcgggaa
     1021 tcccgaggcc ctcaactggg ccgtcgacgt ggtcgccagc ctggtcgagg actacggaat
     1081 ggattggtac gaagaggact tcaacctcga tcccaacccc tatctggaag ccgccgccga
     1141 gccggaccgc tggggcatga gagagatgcg gttcatcgag ggccacttcg ccttctggga
     1201 cacactcctc cagcggttcc cgaacctggc gatcctcaat tgtgcgagcg gcgggcgccg
     1261 catcgacctg gagtcgatct cgcgcgggga gccgctctgg cggagcgact acaactgctt
     1321 cccggaagcg acggccgaat cgacgcagga ccagagctac gggatccagc actggctgcc
     1381 gatccagggc agcatgatcc gtggctggtc gatgtacgac acctacgagg cccgatgcgc
     1441 gctgagcccg ggcatggaga cgggcatcgt ggccaagacg ccggaggaat ggcttcagct
     1501 caaggccaac gtcatccagg cgaagcgctg caagaggtac ttcatgggcg actactaccc
     1561 gctggcgaac cagcagcgcg acccggcggc gtggacggcg taccacctgt tcctccccga
     1621 ggagcaggag gggatgctcg tggcgctgcg gcgggccaag agcgacgtgg cggccatgac
     1681 gttcgacctc ttgaccatcg acccggcgag gcagtgggag ttcgaggact acgacagcgg
     1741 caagaggtgg gtggcctcgg ggagacagat tcgcgaggaa ggcttcgagg tcgcgatcac
     1801 gcagcggcgc gggtcgcggc tgatcttcta ccgtccggcg ccgtagggcg gaccaccaaa
     1861 ggccaacacc gcgggactct gtcccgggcc ctgccagggg accagtcccc tggaccccat
     1921 ccggctgccg cccgggctcc aacgggccct gctccggcgt cgcgcgatgc ggccatcccg
     1981 cttcgcggtc cgcgccagtc ggccccctcg gagacacctc cggcgcctcc gtgaggccga
     2041 ctcggcgcgg ccaggcagag cccgggacgg ccgcatcgcg cggggctgcg gggaacgccg
     2101 tataggggcg accgcgcgtc gggaggacgg gggacggagc actctgccac ggcgggcgcc
     2161 ggccgagcga ctgcccgctt tggcagaggg ctctgtcccc tctgtcgcag actcactgag
     2221 ggcgcgcccg ggattcgtca gtgacgggcg gggtgtacgg
//
LOCUS       DAOSPL010000803         2260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47884_length_2260_cov_2.776871, whole genome shotgun sequence.
ACCESSION   DAOSPL010000803 DAOSPL010000000
VERSION     DAOSPL010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47884_length_2260_cov_2.776871"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..827)
                     /locus_tag="PLQ54_13860"
     CDS             complement(216..827)
                     /locus_tag="PLQ54_13860"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction ATP-dependent DNA helicase
                     RuvA"
                     /protein_id="HOS94400.1"
                     /translation="MISYLRGDLVSVNDEGVVLDVAGVGYDVLVAPIALDHLRTIELG
                     TEVVLLTHHYLQLEQSRGTPVLMGFRTAEERDLCLALMAVIGPRSALKALAAPVGQIA
                     QSIEFGDVAYMKSLPGIGPQKAKEIIAKLQGRVARFIGVAVEPAVAVAQHGADDPVSE
                     AIDALVSLGHKRAEAVQAVNRALRDDPTLSSTQAIIGQVYAQR"
     gene            complement(1160..1663)
                     /gene="ruvC"
                     /locus_tag="PLQ54_13865"
     CDS             complement(1160..1663)
                     /gene="ruvC"
                     /locus_tag="PLQ54_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOS94401.1"
                     /translation="MTIAGIDPGLNATGYGVIRTDAMGVAWLGEGGTIRPTAKATLEC
                     RLQELHRGIREVLEEHRPNVVVVEELYSKYEHPRTAILMGHARGVVYLAAAELGLTVV
                     GYTASTVKRAVTGSGRASKDQVQLMVQRALRLAEAPQPDHVSDALALALCHANTERPV
                     LGGQLKR"
     gene            complement(1709..>2260)
                     /locus_tag="PLQ54_13870"
     CDS             complement(1709..>2260)
                     /locus_tag="PLQ54_13870"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOS94402.1"
                     /translation="SGPISGMTLAELKRLDAGSWKDAAYAGERVPTLAEALRLIGDRA
                     GLIIEIKQPGIEQAVVDVVRAEGALRRVFVFSFHHESVKRVRAIEPLIPCGWLTGGTQ
                     DPSDEAAAALVDQVLTAHASMLASHYQGVTDAVKRRVALAGLTLCVWTLDEEEDLRTW
                     VAAGADAIGSNWPNKLQAVLREG"
BASE COUNT          375 a          850 c          672 g          363 t
ORIGIN      
        1 acagccacga tcgccgcggt ccgctcggcg accgacatcg tgtcgccgcc accgttgttg
       61 ttaccgttgt tgttgtcgcc gttccctggc ataccgcccc cgcagccggc gatacccatg
      121 atcagcccaa gcacaaacgt gaccccaaac gcggttctca tcttcggccc cctcgtccct
      181 gacccctgat cgatcactgc cgtccgccgt atgcatcacc gctgcgcgta cacctgcccg
      241 atgatcgcct gggtcgacga aagcgtgggg tcatccctca gtgcgcggtt caccgcctgc
      301 acagcctcgg cacgcttgtg gccgaggctg accagtgcgt cgatggcctc cgacaccggg
      361 tcgtcggccc catgctgagc cacggccacc gcgggttcga cagcaacgcc gatgaaccgg
      421 gccactctgc cctgcagctt ggcaatgatc tccttggcct tctgcggtcc gatcccgggc
      481 agggacttca tataggccac atcaccaaac tcaatgctct gagcaatctg ccccaccggt
      541 gcggccaatg ccttgagcgc actccgaggc ccgatcacag ccattagtgc caggcacaag
      601 tcccgttcct cggccgtgcg aaatcccatc agcaccggcg tgccgcgcga ctgctcgagc
      661 tgcaggtagt ggtgggtgag cagcacgacc tcggtgccca gctcaatggt gcgaaggtga
      721 tcgagcgcaa tgggcgccac gaggacgtcg taacccactc cggccacgtc gagcaccacg
      781 ccctcatcgt tgaccgagac caggtcgccg cgtagatagc tgatcaatgt tggaggctct
      841 ccctacccga gcggcagcga gtcgtcaccc gaatggtccg cacccagggc cccggccgac
      901 cccgaagcct acgttacagc acagacaacg gtgtcatcct cagcggagca cccgcggagc
      961 gaaggatctc ggctcgaact ggctgaccac cgaagacacg aacctgtgcc agcgcaacga
     1021 ggcggctgcc agccgagatc cttcgtcgct ggcgctcctc agggtggcac attggcttgc
     1081 cgcgccgcgg ggcgttccgg gctacctcac ggcgccgtct gcctcaggtc ctgacccctt
     1141 ctgccccccc ttcccgcccc taccgtttca actgcccccc cagcaccggc cgctccgtgt
     1201 tcgcgtggca cagcgccagc gccagcgcgt cggacacgtg atccggctgc ggcgcctccg
     1261 ccagccggag cgcccgctgc accatgagct gaacctgatc cttgctcgcc cggcccgagc
     1321 cggtgaccgc ccgcttcacc gtcgaggccg tatagccgac caccgtcagg cccagctccg
     1381 ctgcggccag gtagaccacg ccgcgggcgt gacccatcag aatggctgtg cgcggatgct
     1441 cgtacttcga gtagagctct tcgacgacga ccacgttggg acgatgctcc tcgagcacct
     1501 cacggatgcc acggtggagc tcctggagcc ggcactcaag ggtggccttg gccgtcggcc
     1561 ggatggtacc gccctcgcca agccacgcca cgcccatggc gtcggtgcgg atcacgccgt
     1621 agccggtcgc gttgagccct gggtcaatgc cggcgatggt catgggatgc gaaggacccc
     1681 tccccgtccg cagtatacca gacgcgcctc agccctcccg gagcaccgcc tgtagcttgt
     1741 tgggccagtt gctgccgatg gcatccgctc ctgcggcgac ccacgtacgc aagtcttcct
     1801 cctcgtcgag tgtccacacg cacagggtga gcccggccag cgcgacgcgc cgcttcacgg
     1861 catcggtcac accctggtag tgggaagcca gcatcgacgc atgggccgtc agcacctgat
     1921 ccaccagcgc cgcggccgcc tcgtcggacg gatcctgggt cccgccggtg agccaaccgc
     1981 aggggatcag cggctcgatg gcgcggaccc gcttcaccga ctcgtggtgg aacgagaaca
     2041 cgaacacccg gcggagcgcc ccctcggccc ggaccacatc gacgaccgcc tgctcaatgc
     2101 ccggctgctt gatctcgatg atcagaccgg cgcggtcacc gatgagcctc agggcctcag
     2161 ccagcgtcgg cacgcgctca ccggcgtagg cggcatcctt ccatgagccc gcatcgagcc
     2221 gcttgagctc ggccaacgtc atccccgaga tcggccctga
//
LOCUS       DAOSPL010000804         2259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47905_length_2259_cov_3.993194, whole genome shotgun sequence.
ACCESSION   DAOSPL010000804 DAOSPL010000000
VERSION     DAOSPL010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47905_length_2259_cov_3.993194"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="PLQ54_13875"
     CDS             <1..974
                     /locus_tag="PLQ54_13875"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOS94403.1"
                     /translation="RTETMAGRRLPPQRTTDLVRETLVASGLSEAWTFSLVAPDAIDR
                     LALGADDPRQRAIAVSNPLTADASALRTMLAPCLLQVAERNARYRNTDLALFEVGRVY
                     RPGTDGEGPTERRAVCILLTGRTGLTGWNVPAEASRADFYALKAIVESLTNELSGGDI
                     AIRPAEGMPFRPGACAAVFWGDDEIGRFGALHPKVQSGYDLPNEVFLAEIDIEGFVGR
                     PRPVPRYQPIPRFPDVTRDLALVLDHAITSAQVEGVIRAAGGPHLVQAEVFDVFEGAQ
                     VGAGKKSIAYSLRFRSPEGTLTDEQVSEAIDSICAALAAELGATLRA"
     gene            1064..1963
                     /locus_tag="PLQ54_13880"
     CDS             1064..1963
                     /locus_tag="PLQ54_13880"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HOS94404.1"
                     /translation="MHIDQATQLFIDHLVVERQSSPHTIAAYAGDLADLAAFVVGEDA
                     AGGLELLTSDRCLRYAHSLRTRGLEPATIARRLSAARSFTKYLVAEGHLNELPFASVP
                     IPKRPGRLPFTLSVDDVKRLLAQPDGDEPVQQRDRCVLTVLYSSGMRVSELIGLRMGD
                     VAFEGGFVRCRGKGGKERMVPLGRVALAELDLYLLRSRPALVSSPSESALFVSNRGRR
                     FSRSGLFYLVKRHALRAGLDSTRISPHTLRHSFATHLLEGGASLRAIQEMLGHANIAT
                     TELYTHVTAEFLREEYSLTHPRA"
     gene            complement(2020..>2259)
                     /locus_tag="PLQ54_13885"
     CDS             complement(2020..>2259)
                     /locus_tag="PLQ54_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS94405.1"
                     /translation="QSDDHIGNWLQCIRTRERCIADVEIGHRSASICHLGNIARLLGR
                     RLHWDPVAEQFIGDDEANSHLDRPRRNGYELPTRI"
BASE COUNT          333 a          783 c          749 g          394 t
ORIGIN      
        1 cgcgcactga gaccatggcc gggaggagac ttccgcccca gcggacgacg gatctggttc
       61 gcgagaccct tgtcgccagc gggctatcgg aagcctggac cttcagtttg gtggctccgg
      121 atgccatcga ccgccttgcg cttggcgccg atgacccgcg ccagcgggcc atcgcggtct
      181 cgaacccact cacggccgat gcctcagctc tgcggaccat gctggcgccc tgcctgctgc
      241 aggtggccga gcgcaacgcc cggtaccgga acaccgatct ggcgttgttc gaggtcggac
      301 gcgtgtatcg gccgggcact gacggggagg ggcccacaga gcggcgcgcg gtgtgcattc
      361 tgctgacggg acgaacgggc ctcacgggct ggaacgtccc ggccgaggcg tcacgcgcgg
      421 acttctacgc actcaaggcc atcgtcgaat cgctcaccaa cgagctctcc ggcggcgaca
      481 ttgccatccg cccggcagag gggatgccct tccggcctgg tgcctgcgcg gcggtgttct
      541 ggggcgatga cgagatcggc cgctttggtg cgctccaccc caaggtccag tcgggctacg
      601 acctgccgaa cgaggtgttc ctggccgaga tcgacatcga ggggtttgtc ggcaggcctc
      661 gtcccgtgcc acgctaccag ccgattcccc ggttcccaga tgtgacgcgc gatctggcgt
      721 tggtgctcga ccacgctatc accagtgccc aggtcgaggg cgtgatccgt gcggccggcg
      781 ggccgcatct ggtgcaggct gaggtctttg atgtcttcga gggcgcccag gtcggcgcgg
      841 gcaagaagag cattgcctac tcgcttcgtt tccggtctcc ggagggcacg ctgaccgacg
      901 aacaggtgag cgaggccatc gactccatct gtgcggcgct ggctgccgag ctgggcgcga
      961 cgctgcgagc ctagctgggc tggcatcgcc aacgcagccg tgctgacggg cggcgcggga
     1021 ccgtgcctgc acaggcaccg ggcgtaggag cactggcgtg cccatgcaca tcgatcaggc
     1081 cacccagctc ttcatcgacc accttgtcgt ggagcgccag agctcgcccc acacgatcgc
     1141 cgcctacgcc ggcgacctgg cggacctggc ggcgttcgtc gtcggcgagg acgcggccgg
     1201 cgggctggag ctgctcacct ctgaccgctg ccttcgctac gcacactcgc tgcggacccg
     1261 cgggctcgaa cccgccacca tcgcccgcag actgagcgcg gcacggagct tcaccaagta
     1321 cctggtcgcg gaaggccatc tcaacgagct tcccttcgcg tcggttccca tccccaagcg
     1381 ccctggccgc ttgcccttca cactttccgt cgatgacgtc aagcgtctgc tggctcagcc
     1441 cgacggcgat gagcccgtgc agcaacgcga tcgctgcgtg ctcaccgtgc tctactcgag
     1501 cggcatgcgt gtctccgagc tcatcgggct caggatgggc gacgtggcgt tcgaaggggg
     1561 ctttgtccgt tgccggggca agggaggcaa ggagcggatg gtccccttgg gccgcgtggc
     1621 gctggcggag ctcgacctct acctcctcag gagtcggcca gcattggtct cttcgcccag
     1681 cgagtccgct ctattcgtgt caaaccgggg ccgtcgcttc tcgcgctcgg gtttgtttta
     1741 cctcgtgaag cgccatgcgc tgcgtgcggg cctcgactcg acccgaatca gcccacacac
     1801 gctgcggcac tccttcgcga cgcacctact cgagggcggc gccagcctcc gggccatcca
     1861 ggagatgctc ggccatgcca acattgccac gacggagctc tatacccacg tcaccgccga
     1921 gttcctccgc gaggagtact cgctgaccca cccccgagcc tgaaggcgcc ccgcgcgatc
     1981 gtcccaggcc atgcctggct ggtcgggagc agcgtggggc tagatgcgtg tcgggagctc
     2041 gtagccattc cgcctggggc gatcgaggtg tgagttggcc tcgtcgtcac caatgaactg
     2101 ttcggctacc gggtcccagt gcagtcgcct gccaagcagc cgggcgatgt tccccaggtg
     2161 gcagatgcta gcgctccggt ggccgatctc cacatcggcg atgcaccgct cgcgcgtccg
     2221 gatgcactgc agccagttgc cgatgtggtc gtcactctg
//
LOCUS       DAOSPL010000805         2259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47912_length_2259_cov_3.276770, whole genome shotgun sequence.
ACCESSION   DAOSPL010000805 DAOSPL010000000
VERSION     DAOSPL010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47912_length_2259_cov_3.276770"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLQ54_13890"
     CDS             complement(<1..398)
                     /locus_tag="PLQ54_13890"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HOS94406.1"
                     /translation="MAAGTAALGLAELAMASAASAAPLDHLPSPDLSALRRYPVGRPR
                     VYTGAHTEHVRMPIGGIGTGTVWLDGDGKLGVWQIFNNYGEDRIPDSFFAVRVAAEGD
                     DPVLRVLQTAPEPGFPAFPDLEYEGGYPIAR"
     gene            642..1283
                     /locus_tag="PLQ54_13895"
     CDS             642..1283
                     /locus_tag="PLQ54_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94407.1"
                     /translation="MSLVYSIDSVRRRLRGEHRVLSQTGSGAPSGDFDQALRAHLARV
                     DRVLADLERPRREDESGTSALRAGLEAGIQEAAQWTQERCRAFHQTLRPFVEATGADL
                     HSEIVLTLDGMGRVRVASPHPEASAIEELFANQPPLRYEFISLAAGSEFVRVAEEYLT
                     FLSLGGRSRAEASERYARRSAGSTPLKFHMGLRGQSYRTFFAATHVPYPLGTV"
     gene            1637..1867
                     /locus_tag="PLQ54_13900"
     CDS             1637..1867
                     /locus_tag="PLQ54_13900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOS94408.1"
                     /translation="MPVYDYRCNRCAKPFEIRCGIKDSRKGVRCPTCNGRDVTQTLGS
                     FTALGLSSKRKSRGSGSSCSSCSKSSCSGCSH"
BASE COUNT          347 a          768 c          802 g          342 t
ORIGIN      
        1 cgtgcgatgg gatagccgcc ctcatactcg aggtcgggga acgctgggaa cccgggctcg
       61 ggggcggtct ggagaacgcg caggaccgga tcatcaccct cggccgcgac ccgcacggcg
      121 aagaagctgt cggggatacg gtcctctccg tagttgttga agatctgcca gaccccgagc
      181 ttcccgtcgc catcgagcca cacggtaccg gtgccgatcc caccgatcgg catccgcacg
      241 tgttcggtgt gagccccggt gtagacgcgg gggcggccca ccgggtagcg cctcagggcg
      301 gacaggtcgg gactcggcag atggtcaagc ggcgccgcgc ttgcagccga ggccatggcc
      361 agctcggcga ggccgagggc agccgtgccg gcagccaccc tgcggaggaa tcctcggcgg
      421 tttaccgccc cgggctcgcc gcaattgcgg ccgtcgtggc agcagtctcc tgacattcag
      481 cctgcaactc ccttcgcacg aaccaccagg tacccagttc ggtggcccag ggccccgacc
      541 tgccgcacgc ggtcagatgg ccctaaggcg atggtcgcgg ccggccgaga accgccatag
      601 aggcgccacg cggcgcgtcc ggggacggag gcagcagctc agtgagcttg gtctacagca
      661 tcgacagcgt gcgccgccgc ctccggggcg aacaccgcgt cctgagccag acgggctccg
      721 gcgcaccctc gggcgacttc gaccaggctc tccgggcgca cctggcgcgc gtcgaccgcg
      781 tactggccga tctggagagg ccccggcgcg aggacgagtc tggcaccagc gcgctgcgcg
      841 cgggcctgga ggctggcatc caagaggcgg cgcagtggac tcaggagcgt tgccgcgcgt
      901 tccaccagac gctgcggccc ttcgtggagg ccaccggagc cgacctccac tccgagatcg
      961 tcctcactct ggacggcatg ggacgagtcc gtgtggcgag cccccacccg gaggcatcgg
     1021 ccatcgagga gctcttcgca aaccagccgc cgttgaggta cgagttcatc tccctcgctg
     1081 cgggatccga gttcgtgcgc gtggctgagg aatatctgac attcctatcg cttggtggaa
     1141 ggagtcgcgc cgaggcctcc gagcgctatg cgaggcgatc agccggttcg acgcctttga
     1201 agttccacat gggcctgcgc ggtcagtcct atcggacgtt cttcgccgcc acccatgtcc
     1261 cgtaccccct tggcaccgtg taggggttgc ccgtgcgggc attggcggca acccggggct
     1321 ccagcggccc atgggcctgg ttgcggccga ccgcgtgggc cgaccgtccc ggtcggctat
     1381 gaggcatcgc gcgaggcact cggcctacgc gcccttgtgc gcccccctgt ccagcccctc
     1441 cacgacacag ggaagtgcgg tgtgtgggac ttgcgcgcag cgcccaggcc tcgatggcgg
     1501 cgagtggccc ctgccgcccc ccccacgggc gcagagcctt gccctcaacc cgcctgccgc
     1561 gtacaactgt cctcagggaa gtccgagggg tccaggcgcg tgccccgact gtcggtgggg
     1621 aggatgagcg gcacctatgc cggtgtatga ctaccgctgc aaccgctgtg ccaagccttt
     1681 cgagatccga tgtgggatca aggactcgcg caagggggtg aggtgcccca cctgcaatgg
     1741 tcgggatgtc acgcagacgc tgggcagctt cacggcgctc gggctctcat cgaagcgcaa
     1801 gtcccgcggc agcggctcct cgtgctcctc gtgctcgaag tcgagctgct caggctgttc
     1861 gcattaggcc aactgccacc aggcgggagg agcgcactgg cgggctgacg aaacactgcg
     1921 tacccgcggg cctggcggcg ccgcggccca aaccctgggt gcggacagag cgtacgccgg
     1981 tttcggtgag cggcgccggg cgatggcgca gcggaggccg tggtgtgcgc gggtccggac
     2041 ggctcggctg ggactcagcc acgactgcga atcgcgggga ggcctcgtga tgcacgggcg
     2101 cgacgacgat gacctcgatc tcatacccct gaatgaggcg ggggaggacg aagacagcgg
     2161 gacgggtctc cagtttgggg attatcggca ggactcgatc tcgccgccca ctgcggccgg
     2221 cgatgaggcg tctgagccgc agggctcgat gctggcggg
//
LOCUS       DAOSPL010000806         2258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47951_length_2258_cov_3.037222, whole genome shotgun sequence.
ACCESSION   DAOSPL010000806 DAOSPL010000000
VERSION     DAOSPL010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47951_length_2258_cov_3.037222"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /gene="metK"
                     /locus_tag="PLQ54_13905"
     CDS             <1..968
                     /gene="metK"
                     /locus_tag="PLQ54_13905"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOS94409.1"
                     /translation="KAQAAIADVARAVIRDIGYERAEWGFDCNTSGVMVAVQRQSPDI
                     AQGVDTGGAGDQGMMFGYATDETPELMPMPILYAHRLARRLAEVRKNRTLPYLRPDGK
                     SQVTIEYADGRPVRVDAVVIAAQHSPDVTRDQIREDVLEHVVKPVLPAAMCDGRLKDF
                     VNPTGSFVVGGPAGDTGLTGRKIIVDTYGGLGRHGGGCFSGKDPTKVDRSAAYMVRYL
                     AKNVVAAGLARRCEIQVAYAIGEPQPVSIYVDSFDTATVDEAKISRALRDMFDLSPRG
                     IIKHLKLDRPIYRQTAAYGHFGRTEPDFTWEKTDVADELRKACGG"
     gene            complement(1003..>2258)
                     /locus_tag="PLQ54_13910"
     CDS             complement(1003..>2258)
                     /locus_tag="PLQ54_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94410.1"
                     /translation="REYLGYYLDRFVNDDGTFAYYGPAVSEYGQMLDVAVRLARCSGD
                     HGWLRTYLGKLDAMAGHLLRLRDRALADPGRSGATHGLLYGDPEADVLDAPNLYFSNN
                     AWAWRGLVSYGHWLAEQPDGAMQRRGAEMVEQARGMGGDTRAAVRAATVPSTPAFVPP
                     YPGIAEPFGSMTEGRLESYTNYRYWPEMLDAGLLDADTVDAILDFRRAHGGELLGTTR
                     FERQMDDWPFARVAYALLERGRVDQYLLGLYGHLAHHQMRGTFTAYEQVDIGWQDERR
                     YRADYCVPAQLTIPLMLKWALVFEERDSDVLWLAKAVPARWFAPGSSFSVDAAPTRWG
                     PVGCMMRCVDHAVLWDLKLPAGPEVRLSMPTEGFAGLIEAPEACTADEATGTIVVSPG
                     ASPRPMRVVARIRGQAGASGVLREP"
BASE COUNT          406 a          800 c          724 g          328 t
ORIGIN      
        1 ccaaggccca agccgccatc gccgacgtgg cccgagcagt catccgggac atcggctatg
       61 agcgcgccga gtggggcttt gactgcaaca cgagcggcgt catggtcgcc gtccagcgcc
      121 agtcaccgga catcgcccag ggcgtcgaca ccggtggcgc cggcgaccag ggcatgatgt
      181 tcggctacgc caccgatgag acgccggagc tcatgcccat gccgatcctc tacgctcacc
      241 ggctggcgcg acgcctggcc gaggtgcgca agaaccggac gctgccgtac ctgcggcccg
      301 atggcaagag ccaggtgacc atcgagtacg ccgatgggcg gccggtccgg gtcgatgccg
      361 tggtcatcgc tgcccagcac agccccgacg tgactcgcga tcagatccgc gaggacgttc
      421 tggagcatgt ggtgaagccc gttctgcccg ccgccatgtg cgacggccga ctgaaggact
      481 tcgtgaaccc caccggtagc ttcgtggtcg gtggtccggc cggcgatacc ggactgacgg
      541 gccgcaagat catcgtcgac acctatgggg gactcgggcg tcacggcggc gggtgcttct
      601 cgggcaagga ccccaccaag gtcgaccgct cggccgccta catggtccgc tacctggcga
      661 agaacgtggt ggccgccggc ctggcgaggc gttgcgagat ccaggtggcc tacgccatcg
      721 gcgagcccca acccgtctcg atctacgtgg actccttcga caccgccaca gtagacgagg
      781 ccaagatcag ccgggccctg cgggacatgt ttgacctctc gccccgcggg attatcaagc
      841 acctcaaact ggaccggccg atctacagac agacggctgc ctacgggcac ttcggccgca
      901 ccgagcccga cttcacgtgg gagaagaccg atgtggccga tgagctccgc aaggcttgcg
      961 gaggttaggg ctgcaccgaa cccgccgaag accgagggca ggctagggct ctcgcaacac
     1021 ccccgaagct ccagcctgtc ctcggatccg ggccaccacc cgcatcggtc ggggactcgc
     1081 gcccggcgat accacgatcg tcccggtggc ctcgtccgcc gtgcacgcct cgggtgcctc
     1141 gatcaagcca gcgaatccct ccgtcggcat cgacagccga acctcgggcc cggcaggcag
     1201 cttcagatcc cagagtaccg cgtgatcaac acaccgcatc atgcacccaa cgggccccca
     1261 tcgggtcggc gccgcatcaa ccgagaatga cgaccccggc gcgaaccatc tcgccggcac
     1321 cgccttggcg agccagagca cgtccgaatc gcgctcctcg aacaccaggg cccacttcag
     1381 catcagcgga atggtgagct gcgccggcac gcagtagtcg gcacgatagc gccgctcatc
     1441 ctgccagccg atgtcgacct gctcataggc cgtgaacgtc ccccgcatct gatggtgggc
     1501 cagatggccg tagagaccga ggaggtactg gtcgaccctc ccgcgttcga gcaacgcgta
     1561 ggccactcga gcgaagggcc agtcgtccat ctgacgttcg aaccgcgtgg tgcccagcag
     1621 ctcgccgccg tgggcgcggc ggaaatcgag gatggcgtcg acggtgtccg catccagaag
     1681 gcccgcatcc agcatttcgg gccagtagcg gtagttggtg taggactcga gccgcccttc
     1741 cgtcatggac ccaaagggct ccgcgatgcc cgggtagggc ggcacaaacg cgggcgtcga
     1801 cggcaccgtg gccgcccgaa cggccgcccg agtgtcgccc cccatgcctc gagcctgctc
     1861 caccatctct gccccgcgcc gctgcatcgc gccgtcgggc tgctcggcca accagtgccc
     1921 ataggagacc agaccccgcc aggcccatgc gttgttcgaa aagtacaggt tgggcgcatc
     1981 gaggacgtcc gcctcgggat cgccgtagag cagcccgtgt gtcgcccccg accgaccggg
     2041 gtccgccagg gcccgatccc tcagcctcag cagatgacca gccatggcgt ccagtttgcc
     2101 gaggtaagtg cggagccatc catggtcgcc cgagcagcga gccagccgca cggccacatc
     2161 gagcatctgg ccatactccg agaccgcggg accatagtag gcgaaggtgc cgtcgtcgtt
     2221 gacgaaacgg tcgaggtagt aaccgaggta ttcccgtg
//
LOCUS       DAOSPL010000807         2258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47957_length_2258_cov_2.563323, whole genome shotgun sequence.
ACCESSION   DAOSPL010000807 DAOSPL010000000
VERSION     DAOSPL010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47957_length_2258_cov_2.563323"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PLQ54_13915"
     CDS             <1..530
                     /locus_tag="PLQ54_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94411.1"
                     /translation="DLMFAFNGYAPAKTVFGASSGYEDLGGTHPGPKTAESLITFTNG
                     VRAALHSGAGAPQFREGPDWAHKRIAVYGTHGFRHWWMYGYELSRPDGSVERGEQEYR
                     DQDILGQAGLTEALFDWIEDDAKVHPNNLSTSLDEWLVILAGYLSTIEARPVGLPFDP
                     PDDLLDRFRAFVGGA"
     gene            566..1792
                     /locus_tag="PLQ54_13920"
     CDS             566..1792
                     /locus_tag="PLQ54_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxyltransferase"
                     /protein_id="HOS94412.1"
                     /translation="MSDPWHTASWFTSPWNHLPEVRSGYHFADQVRIHDITLRDGEQQ
                     TGVALSADEKVRIAEKLAEVGVHRIEAGMPIVSAADRQAIERIVAAGFPCEIFAFSRC
                     MVADVERAAECGVHGIVVEIPSSEHIIEHAYGWPLQKAVDLSIAATARAKELGLYTVF
                     FPIDASRADLGWFLDLISQVAAQGHMDALACVDTFGGVNPSAIGNLVATCKRHLGKPI
                     ETHFHDDFGCGTANTLLGLAAGAEVAHVTMCGVGERAGNAALEQVVLALKTQYGIDVG
                     IDTTKLCELAALVQETFHHALPDNRSIVGKRLFQVESGIVASWLKRCGDEHLLELFPY
                     RPELVGQEAPSVAMGKSSGADSVTAWLADLGLEPPTDDAEAMRILVAVKAASIEKHGT
                     LTPDEFRRIVANERGA"
     gene            1856..>2258
                     /locus_tag="PLQ54_13925"
     CDS             1856..>2258
                     /locus_tag="PLQ54_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94413.1"
                     /translation="MSSALCLLSLIAAAGQALTNIYPDPGFEKTGVVGVAHSGERAGT
                     LEVGERQHWVTLDGSLSVEPFATYRATAWVRGGAAEGSALALYVYQWDSYVWAFGAQA
                     VVTAGDTWQQVSVDFCVPTDAVPFHPLAFMDA"
BASE COUNT          362 a          759 c          742 g          395 t
ORIGIN      
        1 tggacctgat gttcgccttc aacggctacg cacccgcgaa gaccgttttc ggggcctcgt
       61 cgggctatga ggacctgggc ggcacgcatc ccggcccgaa aaccgccgag tcgctcatca
      121 ccttcaccaa cggcgtccgc gcggcccttc actcgggcgc gggcgcgccg cagttccgcg
      181 aaggtcccga ctgggcacac aagcgcatcg cggtttatgg cacccatggc ttccgccact
      241 ggtggatgta cgggtacgag ctctcgcggc ccgatggttc cgttgagcgc ggagagcagg
      301 agtaccggga tcaggacatc ctcggccagg ctggcctcac cgaggccctc ttcgactgga
      361 tcgaagacga tgccaaggtc caccccaaca atctgagtac atcgctggac gaatggctcg
      421 tgattctggc aggctatctg agcaccatcg aggcgcgtcc cgtgggcctg cccttcgatc
      481 cgcccgatga cctgctcgac cgcttccgcg ccttcgtggg cggcgcctga cgcgcccccg
      541 gcctgctgag acgagaggac gacacatgtc cgacccgtgg cataccgcgt cgtggttcac
      601 gagcccatgg aaccacctgc cggaagtccg ctccggctat cacttcgcgg accaggtaag
      661 gatccacgat attacgctcc gggacggcga gcagcagacc ggcgtcgccc tgtcggccga
      721 tgagaaggtg cgtatcgccg agaagctggc ggaggtgggc gtgcaccgga tcgaggcggg
      781 catgcccatc gtgtctgccg ccgatcgtca ggcgatcgag cgcatcgtcg ctgccggctt
      841 tccgtgcgag atcttcgcct tctcgcgctg catggtggcc gatgtcgagc gcgccgccga
      901 gtgcggcgtg catggcatcg tcgttgagat ccccagcagc gagcacatca tcgagcatgc
      961 atacggctgg ccgctccaga aggccgtgga cctgtcgatc gctgcgacgg cccgcgccaa
     1021 ggaactgggt ctctacaccg tcttctttcc catcgacgcc tcgcgggccg atcttgggtg
     1081 gttcctcgac ctgatctcgc aggtcgccgc tcagggtcac atggatgctc tcgcctgcgt
     1141 cgataccttc ggtggcgtca acccgtcggc tatcgggaat ctggtcgcca catgcaagcg
     1201 gcacctcggg aagcccatcg agacgcactt ccacgatgac ttcgggtgtg gtacggccaa
     1261 cacgcttctg gggctggccg ccggcgccga agtggcccac gtcaccatgt gtggcgtcgg
     1321 cgagcgcgcc ggcaacgccg cgttggagca ggtggtcctt gcactcaaga ctcagtacgg
     1381 catcgatgtg ggcatcgaca ccaccaagct gtgcgagctg gctgctctgg tgcaggagac
     1441 cttccaccac gcgctgcccg acaaccgctc aatcgtcggc aagcgcctgt ttcaggtcga
     1501 gtccggcatc gtcgcgtcct ggctcaagcg atgcggcgat gagcacctcc tcgagctgtt
     1561 cccctaccgg cccgagctgg tggggcagga ggcgccatcg gtcgccatgg ggaagagcag
     1621 cggcgctgac tcggtcacgg cgtggttggc cgacctcggc ctggagcccc ccaccgatga
     1681 cgccgaagcc atgcgcatcc tggtcgcggt gaaggcggcg tccatcgaga agcatggtac
     1741 gctcacgccg gatgagttcc gccggatcgt ggcgaacgag cgcggagcgt aggtcacgag
     1801 gagagaggag gcaggggcgc gaaccctccc gcgtcatcgg cgaggaggtc gacatatgtc
     1861 atctgcgctc tgtctcctct cactcatcgc ggcagcgggt caggcgctca ccaacatcta
     1921 ccccgacccc ggctttgaga agaccggggt cgtgggggtc gcccacagcg gagagcgcgc
     1981 tgggacactc gaggtcggcg agcgtcagca ctgggtaaca cttgatggct cactgtccgt
     2041 cgagcccttc gcgacctaca gggccacggc gtgggtccgc ggtggcgcgg ccgaggggag
     2101 cgccctcgcc ctctatgtct accagtggga ctcctacgtg tgggcgttcg gcgctcaggc
     2161 cgtggtcacc gcgggtgaca cctggcagca ggtctccgtt gacttctgcg tgcccaccga
     2221 cgccgtcccc ttccatcctc tggcgttcat ggatgccg
//
LOCUS       DAOSPL010000808         2257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47968_length_2257_cov_4.230699, whole genome shotgun sequence.
ACCESSION   DAOSPL010000808 DAOSPL010000000
VERSION     DAOSPL010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47968_length_2257_cov_4.230699"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="PLQ54_13930"
     CDS             <1..1268
                     /locus_tag="PLQ54_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94414.1"
                     /translation="LLLAAGAYTTIRLAVRLRGDAVGTANDWALWVFPRRAGLGLPAS
                     VRVGQVGLASATGAFPEVEPVARDEAAGAPHSALVARALDAGLVEYVARGGHLLLLDP
                     GPLMPTESVTFEPAWWLGSPGADSHAGTVIRPGPPLDDFPHEGFCDLQFYSLTHERPA
                     MLLDDLPVHVRPIIRAIDAYTGGRSKAFLLEFRVGQGVLMVSTLDLSASALRQRPEAR
                     WMLRSLLRHLGSGQLEPEAALTASFVRDAVGTARVPADTLWAEGYSRTLGHRGERVEH
                     RSVWEDAAPCEVVRCTDGTQFVEWLTGPAPVGPAGNTVTFRWAGGLGWLTEPESAFTL
                     SIDGQPALQFGVTQRSATWDSPDGSIRLTYEVTATFGPDSIGLFSLSVPRAVCPPGSS
                     LRLRITGSPSGSRRWFILHGYGNALERDW"
     gene            1754..>2257
                     /locus_tag="PLQ54_13935"
     CDS             1754..>2257
                     /locus_tag="PLQ54_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS94415.1"
                     /translation="MRRRGFTLIELLVVIAIIAILAAILFPVFAKARAKARQASCASN
                     LKQLALGILQYCQDNDETYPLGNFGNTGWVFYTTPWDARPGSPSLRGAFWASSTQAYI
                     KNWQILQCPSTNATLFSGWANPAPDKDFPISYTYNGLLNAYPMAGVTAPAKCIMLFEG
                     NGVLALRS"
BASE COUNT          352 a          742 c          772 g          391 t
ORIGIN      
        1 tgctcctgct cgccgccggg gcctatacca cgatccgcct ggccgtgcgg ctgcgtggcg
       61 atgccgtcgg taccgccaac gactgggcgc tgtgggtctt cccgcgccgt gccgggctcg
      121 gcttgcctgc gagtgtgcgg gtcggacagg ttgggctcgc gagcgcgacc ggcgcgttcc
      181 ccgaggtgga gccggtggcg cgcgacgagg ccgccggagc gccgcactcg gcgctggtcg
      241 cccgcgcgct ggatgccggg ctggtcgagt acgtggctcg cggcgggcac ctactcctgc
      301 tggatccggg gccgctcatg ccgactgagt ccgtgacctt cgagcccgcc tggtggctcg
      361 ggagccccgg tgccgactcg cacgccggaa cggtgatccg gccaggtccg cccctggatg
      421 acttccccca cgagggcttc tgcgatctgc agttctacag cctgacgcac gagcgccccg
      481 cgatgctgct ggacgacctt ccggtccatg tccgacccat catccgggcc atcgacgcct
      541 acacgggtgg ccgcagtaag gcgtttcttc tcgagttccg tgtgggccag ggcgtactca
      601 tggtgtcaac gctcgacttg agcgcatccg ctctgcgcca gcgccccgag gcccgctgga
      661 tgctgcggag cctgctgcgt catctgggtt caggccaact ggagccggag gcagcgctca
      721 cggccagctt cgtccgcgat gcggtgggca cggcccgggt gccggccgat acactctggg
      781 ctgagggcta ctcgcgaaca ctgggccacc gtggcgaacg cgtcgagcac cggtcggtct
      841 gggaggacgc ggcgccctgc gaggtggtcc gatgcaccga cggcacccag ttcgtggagt
      901 ggctgaccgg ccccgccccg gtggggcccg cggggaacac cgtaacgttc cgctgggcgg
      961 gcggtctggg gtggctcacg gagcccgagt cggccttcac cttatccatc gacggccagc
     1021 ccgcactgca gttcggtgtc acccagcgca gtgccacctg ggactcgccg gacggctcga
     1081 tccgtctgac ctacgaggtg acggccacat tcggaccaga cagcatcggg ctgttcagcc
     1141 tctccgtgcc gcgagccgtg tgtccgcctg gttcgtcgct caggctccgg atcaccggat
     1201 ctccctcggg ctccaggcga tggttcattc tgcatggata tgggaatgct ctggaacgcg
     1261 actggtagcg ctgccgcgtg tctgtagggc ggagcgacgg ccgagcgggc agggcagtgc
     1321 cgttccacag ccagtccggg agcctcctca ggcgcatatg gccctgggcc ggacacggaa
     1381 ggggcccgca tggcgcgatg gggcctaccc agctcacact cgggaacgaa aaggccctgg
     1441 tcaagcggga gggaggtcga gccgatccca tagtgtggtg ctatagttag gatagcctgc
     1501 cgagaagaac cgcgccgtct gctgaagggt acggccgttc gatcggcgaa ggagacctga
     1561 taggagaccg gggactccta tctgcaagaa gggacctggg gggccccgcc aggtttgccc
     1621 attcccaaag caagagaacg catgcctggt gccccgggtt tgcacgagcg atagactctt
     1681 tttcgctatg aatgactgcc tctccccggg ggggcctctg ccgacagggc gagagagtaa
     1741 ggagaggaag ggcatgcgcc gtcgaggatt cacgctgatc gaactgcttg tagtcatcgc
     1801 gatcatagcc attctggctg ccatcctgtt ccccgtgttc gccaaggccc gcgccaaggc
     1861 gcgtcaggcg agttgcgcct cgaacctgaa gcagttggcg ctgggcatct tgcagtactg
     1921 ccaggacaat gacgaaacgt acccgctggg caactttggg aacacgggat gggtgttcta
     1981 caccacgcca tgggatgccc ggccgggatc gccgagtctg cgcggcgcct tctgggccag
     2041 ctcgacccag gcgtacatca agaactggca gatcctccag tgtcccagca cgaacgctac
     2101 tctgttctcg ggttgggcga acccggcacc tgacaaggac tttcccatct cgtacaccta
     2161 caacggtctg ctgaatgcct acccgatggc aggcgtgacc gcgccggcga agtgcatcat
     2221 gctgtttgag ggcaacggcg tgctcgccct ccgcagc
//
LOCUS       DAOSPL010000809         2257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_47974_length_2257_cov_3.368756, whole genome shotgun sequence.
ACCESSION   DAOSPL010000809 DAOSPL010000000
VERSION     DAOSPL010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47974_length_2257_cov_3.368756"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PLQ54_13940"
     CDS             <1..379
                     /locus_tag="PLQ54_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94416.1"
                     /translation="GVAQALRPRGLFIFDLNTIRALEQELFTQKNPNPSDPLQYNWES
                     SYDAEARVCTVRMRFRYEGESGAEEFVEVHRQKGYTHSEIEAWLKDAGFERPRALNAY
                     TFRRASPFANRVFYVTRKARWRR"
     gene            376..813
                     /locus_tag="PLQ54_13945"
     CDS             376..813
                     /locus_tag="PLQ54_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94417.1"
                     /translation="MMRVAAIVMAGLLVAGCGDKKAKTAESADACMKMFVDAIGSGDA
                     EAAADLFDYEAYARAHNEDWDDIATGQRNEIIGKIRADKTASLKAQLGSLQGDLGYTS
                     AGPATGGQAVYSVTANGVPVGNVTVRQTGKGWKVTEAFGSGPA"
     gene            858..2003
                     /locus_tag="PLQ54_13950"
     CDS             858..2003
                     /locus_tag="PLQ54_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94418.1"
                     /translation="MGLSAIGGTAPGVDAGPFAFNQVETHRIRIRNTVGGAVEVSPDA
                     GRSWQAVGSVARPATALAEGFAASRWARDGTVCATAVHGLRLRVGPSMSSGRGGLLAI
                     VPKEFASPPPNYGGFVPGASGIYTDIPAGTSIFRNLAPFLGNIVLREESGSVQRIGPD
                     YRPRVGDVLVIVVARPGRMPQCITLENRSGGRVLADYGGGEVYEFARVRHPVEGVGRF
                     DATGFTGVGCINTNHPGVVTISTAPDHNGKPVFDDPFGETRGGFMIQPARHAESEAAG
                     GGPVAQVMVVEPVSRRLSGGLEGTPPLFQGFLSLQFQSRDPSRSFVCQMRVDDGDWEP
                     LVQYVGIRPGLFASPRELERVLDQQGRGRFVRQGVTAFRILFPEQWP"
BASE COUNT          358 a          715 c          796 g          388 t
ORIGIN      
        1 gggcgtcgcc caagcactga ggccacgagg gctgttcatc tttgacctga acaccatccg
       61 cgcgctggaa caggagctct tcacacagaa gaaccccaac ccgtcggacc ccctccagta
      121 caactgggag agctcctacg acgccgaagc ccgtgtgtgc acggtccgga tgcggttccg
      181 ttatgagggc gagtcgggag cggaggagtt tgtcgaggtt caccggcaga aagggtacac
      241 gcactcggag atcgaagcct ggctcaagga cgcgggtttc gagcggcccc gcgcgttgaa
      301 tgcctacacc tttcggcggg cgagtccctt cgcgaaccgc gtcttctatg tgacccggaa
      361 ggctcggtgg cgtcgatgat gcgggtagcc gcgatcgtca tggccggctt gctggttgcc
      421 ggttgcgggg acaagaaggc caagaccgct gagagtgccg atgcctgtat gaagatgttc
      481 gttgatgcca ttgggtccgg tgacgccgag gcggctgccg atctgttcga ctacgaggcc
      541 tacgcccggg cccacaacga ggactgggac gacatcgcca cgggtcagcg gaatgagatc
      601 atcggcaaga tccgggccga caagaccgct tccctcaaag cccagttggg ttccttgcag
      661 ggcgacctcg gctacacgtc cgccggcccc gccacgggag ggcaggccgt gtactcggtc
      721 acggccaacg gcgtgcctgt cggtaacgtc acggtgaggc agacgggcaa agggtggaag
      781 gtcaccgagg cctttgggag tggtcctgcc tgagcgcatg gccactcatc tgcgcagcgc
      841 gcgcgctgct gcccgcgatg ggcctctccg cgattggcgg cacggccccg ggcgtcgatg
      901 cgggtccctt tgcgttcaac caggtcgaga ctcaccggat ccgcatccgg aacacggtag
      961 gcggggccgt cgaggtcagc cccgatgcgg ggcgatcatg gcaggcggtg gggagcgtcg
     1021 cccggccggc cacggcgttg gccgagggct tcgcggcgtc tcgctgggcg cgcgatggta
     1081 ccgtgtgcgc cactgcggtc catggcctcc gtcttcgagt gggcccttcg atgagctcgg
     1141 ggcgaggcgg gctactcgcc atcgtgccca aggagttcgc cagccctcca cccaactacg
     1201 gtggattcgt ccccggcgcg tcgggcatct acaccgacat tcccgcgggg acttccatct
     1261 tcaggaacct ggctcccttc ctcggcaaca tcgtgttgcg cgaggagagt ggcagcgtgc
     1321 aacgcatcgg gccggactac cgcccccgcg tcggcgatgt gctcgtcatc gtggtggcgc
     1381 ggccggggcg gatgccgcag tgtatcacgc tcgagaatcg ctctggcggc agggtgctgg
     1441 cggattacgg cgggggtgag gtatacgagt tcgctcgggt gcgccacccg gtcgaggggg
     1501 tcggccgctt cgacgccacc ggcttcacgg gcgtgggctg catcaacacc aaccacccgg
     1561 gcgtggtcac catctcgacg gctcccgacc ataacgggaa gccggtgttt gacgatccat
     1621 ttggcgagac gcgaggcggg ttcatgatcc agcccgcgcg ccatgccgag tccgaggcgg
     1681 ccggtggcgg cccggtggcc caggtgatgg tcgtcgaacc ggtcagccgc cgcctctccg
     1741 gtggactcga gggaacacca ccgctgtttc agggcttcct gagcctgcag ttccagagcc
     1801 gcgacccctc gaggagcttc gtgtgccaga tgcgtgttga tgacggtgac tgggaacctc
     1861 tggtccagta tgtcggtatc cggcccggcc tgttcgcatc gccgcgggaa ctcgagcgcg
     1921 ttctcgatca gcagggccgg ggccgctttg tgcgccaagg tgtcaccgcg ttccgcattc
     1981 tgtttcccga gcagtggccg tgagaggtca gaaggcagac aacagagcgg tccactgggc
     2041 ctgagtgggc ggcggcgcaa cccaggggct gttcggccgt ctagtcgcgg gagcccatag
     2101 gagctgaccg ttccgagcgc gaagggggat ccgccccgcg agccggcgca gcacttgcca
     2161 tcgtggcgag tggagggtaa ccaatgggac gcatgaggtg ctcccagtgc ggtcgtgtag
     2221 tcgcaggcac catcgtgtcc ctctgccccg cctgtgg
//
LOCUS       DAOSPL010000810         2253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48060_length_2253_cov_3.394904, whole genome shotgun sequence.
ACCESSION   DAOSPL010000810 DAOSPL010000000
VERSION     DAOSPL010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48060_length_2253_cov_3.394904"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /locus_tag="PLQ54_13955"
     CDS             complement(<1..1411)
                     /locus_tag="PLQ54_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOS94419.1"
                     /translation="MLLTAGRSGAETVVVLAPDRPSLDCSVTFVNDQAREVTLGPFAF
                     SQGQARIQSLPLSRTDRSCTLRVCDRRSGQVARATIDFGPDRITRYRFGTPDFFTPHT
                     LRVALVTRADHAIPAVRVEVTDSANEVRQVVLGAVNRVEVVELHDLHSGPATVRVYAA
                     DLLERERSVRIDSAVPATYTPLLIGSGSAEATPAETEANDAAGMEHQPLIDRLALVMF
                     LLTLTAWGGAGAVLALTPASLGVDRAARMRRAALFAVVVSGVVLILNGLPLVLLGSIS
                     YLPVATCITAVQLMAASWLLLAIQARNRSGLSLATAVLIVAMGGSLLFVPALAVPDDS
                     GGLVLRLSIAQVTLVLAAVAALVVSRVRTSFADDAQRALRERHRQAQKCRKCGEPLHP
                     VTGICRCDLVHTSGSRTRTVARLTVKSGKGAMRHYALGPSVIVGSGPDCDVCLSDDPR
                     VAKSHCLIFERGRDICVRDN"
     gene            complement(1584..>2253)
                     /locus_tag="PLQ54_13960"
     CDS             complement(1584..>2253)
                     /locus_tag="PLQ54_13960"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOS94420.1"
                     /translation="IAERVLQSAREMPVFHIHAEVDATAVLAARAAAKNELGQEAPSY
                     NDFILKAVATTLRRHPRLNAWFENDVIRVAKQVNLAFAAATPEGVLLPTVFEADRKTL
                     EEIAAESREMIAMARAGRLRASLQAGATFTVSNIGPVGIDAFNAIISPPQVAILAIGS
                     VLEKAAVVDGSLGVRSVASFTLTVDHRAIDGADGAAFLADVKKALEAQTWPPAGTEGP
                     TGEG"
BASE COUNT          427 a          766 c          733 g          327 t
ORIGIN      
        1 cgttatcacg cacacagatg tcgcggccgc gctcgaagat caggcaatgg gacttcgcaa
       61 ccctcggatc gtcactgagg cacacatcgc agtccgggcc gctgcccaca atgacggacg
      121 gccccaacgc atagtgcctc atggcgcctt tgcccgactt cacagtaagc cgggccacgg
      181 tacgcgtgcg cgagccgctg gtatggacca gatcgcaccg gcagatcccc gtgacggggt
      241 gaaggggctc gccgcatttg cggcacttct gtgcctgacg atgccgctcg cgcagagcgc
      301 gctgggcatc atcggcgaat gaggtgcgaa cacgagagac caccaatgcc gccacggcag
      361 ccagaaccag agtcacctgc gcgatcgaga gccggaggac cagccccccc gagtcatccg
      421 gcacggccag cgccgggaca aacagcagtg agccgcccat ggccacgatc agcaccgcgg
      481 tggcgaggga aagccccgag cggttgcgtg cctgtatggc cagcagcagc cacgaggctg
      541 ccatgagctg gaccgcggta atgcacgtgg ccactggcag gtaggatatc gatcccagga
      601 gcacgagcgg gaggccattg aggatcagga ccacacccga cacaaccacg gcgaacaacg
      661 ccgctcgacg catcctcgcc gcgcgatcca cacccaaact agccggcgtg agggccagta
      721 ccgcccccgc gccgccccac gccgtgagcg tgagcaggaa catcacgagg gcgagacggt
      781 cgatcagcgg ctggtgctcc atccccgccg catcgttcgc ctctgtctct gccggagtcg
      841 cctcggccga ccccgagccg atcagcaggg gcgtgtaggt ggccggcacg gccgagtcga
      901 tacggacgct acgctccctc tcgaggaggt cggccgcata caccctgacg gtggcggggc
      961 cgctgtgtag atcatggagc tcgacaactt ccacgcggtt cacagcgccc aacacaacct
     1021 ggcgcacctc gttggcggag tccgtgacct cgacacgtac ggcggggata gcgtggtccg
     1081 ctcgggtgac cagcgcgacg cggagtgtgt ggggggtgaa gaaatcagga gtgccaaatc
     1141 gataacgcgt tatgcggtcg gggccaaagt caatagtggc ccgagcgact tggcccgaac
     1201 gcctatcgca gacccgcagg gtacacgagc ggtccgtgcg cgataggggc agggactgga
     1261 tccgggcctg tccttgcgag aatgcgaatg ggccaagagt cacttctcgc gcctggtcgt
     1321 tgacgaacgt cacactgcag tccaggctag gccggtcggg cgccagcaca acgaccgtct
     1381 cggctccgct ccggcccgca gtgagcagca atgccagcgc cgccgaacac cgaaacgagg
     1441 aacacaggag tttggtccgg ccccgttggc catcgcaatg cgcagagccc tgcttcggca
     1501 aggacttggc cagcctccga gtacccgtgg gatcgaggcc gcgcccgtgc accgaacggc
     1561 acaggcgccg ccacagactt ccctcaacct tctccggtag ggccctcggt ccctgccggc
     1621 ggccaggtct gggcctccag ggccttcttc acatcggcca gaaaggccgc accgtcggct
     1681 ccatcgatgg cgcggtggtc gacggtaagc gtgaagctcg ccacggaccg aacgccgagt
     1741 gacccatcaa ccaccgcagc cttctccagg accgagccga tggccagtat cgcgacttgc
     1801 ggggggctga tgatggcgtt gaacgcgtcg atgcccaccg gcccgatgtt cgagaccgtg
     1861 aaagtggcac ccgcctgaag actggcgcga agtcgaccgg cccgcgccat ggcgatcatc
     1921 tcgcgcgact cggcggcgat ctcctccaga gtcttccggt ccgcctcgaa gacggtgggc
     1981 agcagcacgc cctccggcgt cgccgcggca aatgccagat tcacctgctt cgccacccga
     2041 atcacgtcat tctcgaacca ggcattgaga cgcggatggc gccggagagt ggtggcgacc
     2101 gccttgagga tgaaatcgtt gtagctcggt gcctcctgac ccagctcgtt cttcgcggcg
     2161 gcccgggcag ccagcacagc cgtggcatcg acctcggcat ggatgtggaa cacgggcatc
     2221 tcgcgggcgc tctgcaggac tcgctcggcg atg
//
LOCUS       DAOSPL010000811         2253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48061_length_2253_cov_3.298908, whole genome shotgun sequence.
ACCESSION   DAOSPL010000811 DAOSPL010000000
VERSION     DAOSPL010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48061_length_2253_cov_3.298908"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PLQ54_13965"
     CDS             <1..655
                     /locus_tag="PLQ54_13965"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOS94421.1"
                     /translation="VFAADFLLADVYARLCTPGHAPLLRELADAVRRMCEGEIIQTAN
                     EGNLELTEEEYLRAIEGKTASLMAACTSIGAQLGHGSADQVHALKRFGFSLGVAFQIA
                     DDVLDLVGDPARMGKSRGQDLRSGRWTLPLIYALGGMGDGHVSMLRRELTDYWAVDEP
                     RLSSIVEALRATGALSRAIATARDHAATARRALAELPHTSARDLLESLAHAVVARES"
     gene            838..1806
                     /locus_tag="PLQ54_13970"
     CDS             838..1806
                     /locus_tag="PLQ54_13970"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOS94422.1"
                     /translation="MADYRSSAWMALAALLVCWGADASWSAGDAGGPPRPGGRCLPCH
                     GASPTLEAVGESGATASLHVDPLAYQASVHGALDCEACHWRMSDMGEPRGANGESVDP
                     VWLSRLTTSVPDRARRVLERCAPCHRPEADDYARSIHFDAVSGSGTEAPVCSDCHGAH
                     AILRHDDIESSTSQAKVPATCARCHANARIMARYDVRTDTVKTFEESFHGKKHELGSK
                     RAAVCSSCHGGHDIRPATDPGSRLSPANRIATCAACHPGCTEQFALSFGHKPMTAKEQ
                     PLVYWIGVFYRLMIALTIGGMIGYISLDLLRKALSTRGGGGSPPPE"
     gene            1836..>2253
                     /locus_tag="PLQ54_13975"
     CDS             1836..>2253
                     /locus_tag="PLQ54_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B"
                     /protein_id="HOS94423.1"
                     /translation="MRWNGHHRAQHLGVVLSFTALVFTGLPLRYPEAAPSRWIMSLLG
                     GATNAGIIHRVAAVGLLAASAYHVLYVLAMWRAGHRQIHMLPGLKDVRDVFDMLRYFA
                     GLQPHPPKMGRYGFPEKFEYWAMVWGTAIMAATGFMM"
BASE COUNT          341 a          748 c          815 g          349 t
ORIGIN      
        1 cgtgttcgcg gcggacttcc tcctggccga tgtctatgcg cggctgtgca cgcccggcca
       61 tgcgccgctc ctgcgcgaac tggcggacgc ggtccggcgc atgtgcgagg gcgagatcat
      121 ccagacggcc aacgagggga acctggagct gaccgaggag gagtacctgc gcgccatcga
      181 gggcaagacg gcatcgctga tggcggcatg cacgagcatc ggggcccaac tggggcacgg
      241 ctcggcggac caggtccacg cgctgaagcg attcgggttc tcgctgggtg tcgcattcca
      301 gatcgcggac gacgtcctcg acctcgtagg tgatccggcg cgcatgggga agagccgcgg
      361 ccaggatctg cgtagcgggc gctggacact gcccctcatc tatgcccttg gaggcatggg
      421 ggacggtcac gtgtccatgc tcagacggga actgacggac tactgggcgg tcgacgagcc
      481 gcggctgtca tcgatcgtgg aagcactccg ggccaccggc gcactgagcc gagctatcgc
      541 cacggcccgg gaccatgccg ccacggcgcg gagggcactg gccgagctgc cccacacgtc
      601 cgcccgcgac ctgctcgagt cgctggcgca cgccgtggtt gcccgggagt cctagagcgg
      661 cgttcgccag atgcccggcg gagcgtcgga ggccatcggc ggacgcgggc cgaatacgtc
      721 gcgctgaaga tgtccgcccc cgcggtgtgc cacgggggcg ccagggtcac ccacggctcg
      781 gttctgtagg aggagggacc ccgtgccgga ggccgctcgg gcacggatgt gcggttcttg
      841 gcggactatc gaagcagtgc atggatggcg ctggccgccc tgctggtgtg ttggggcgcg
      901 gatgcgtcat ggtcggccgg cgacgccgga ggcccaccgc ggccgggggg acgttgcctg
      961 ccgtgccacg gggcgtcgcc cacgctcgag gcggttgggg agagcggggc gaccgcttcg
     1021 ttgcacgtcg accccctcgc gtaccaggcc tcggtccatg gcgcgctgga ctgcgaggcg
     1081 tgccactggc ggatgtcgga catgggcgag ccccgcggag cgaacggcga gtccgtcgac
     1141 ccggtatggc tgagtcgcct gaccacctcc gtcccggacc gcgcgcggcg cgtgctggag
     1201 cgctgcgcgc cctgtcatcg cccggaggcc gacgactacg cgcggagcat ccacttcgat
     1261 gccgtgtccg gcagcggcac cgaggccccg gtgtgcagcg attgccatgg cgcgcacgct
     1321 atcctgcgac acgacgatat cgagtcatcg accagccagg ccaaggtgcc tgcgacctgt
     1381 gcccgatgcc atgccaacgc gcgcatcatg gcccggtatg atgtgcgcac cgacacggtg
     1441 aagaccttcg aggagagctt ccacggcaag aaacacgagc tgggctcgaa gcgagcggcc
     1501 gtgtgctcga gttgccacgg cgggcacgat atccggcccg cgaccgaccc cggatcgagg
     1561 ctgtcgccgg ccaaccggat cgcgacatgc gccgcgtgcc atccggggtg caccgagcag
     1621 ttcgctctct cgttcggcca caagccgatg acggccaagg agcagcccct ggtctactgg
     1681 atcggcgtct tctatcgact gatgatcgcg ctgacgatcg gcggcatgat cggctacatc
     1741 agcctcgacc tgttgcgcaa ggcgctgagc acgaggggag ggggcggctc gccaccgccc
     1801 gagtgatgaa gcgagcgagt ggggagaggc agttcgtgcg gtggaatggc caccaccggg
     1861 cccagcacct gggtgtggtg ctgagcttca cagcgctcgt gttcacgggc ctgccacttc
     1921 gctaccccga agcggccccc tcgcgctgga tcatgtcgct tctcggcggc gcgaccaacg
     1981 cggggatcat ccaccgcgtg gcggccgtcg gcctgctggc cgcatcggcc taccacgtgc
     2041 tctatgtgct ggccatgtgg cgggctggcc atcgccagat tcacatgctg cccgggctca
     2101 aggacgtccg cgacgtgttc gacatgctgc ggtacttcgc cgggctccag cctcacccgc
     2161 ccaagatggg gcggtacggc tttcccgaga agttcgagta ctgggccatg gtgtggggca
     2221 cggccatcat ggcggccacg gggttcatga tgt
//
LOCUS       DAOSPL010000812         2252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48082_length_2252_cov_3.964497, whole genome shotgun sequence.
ACCESSION   DAOSPL010000812 DAOSPL010000000
VERSION     DAOSPL010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48082_length_2252_cov_3.964497"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2003
                     /locus_tag="PLQ54_13980"
     CDS             <1..2003
                     /locus_tag="PLQ54_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94424.1"
                     /translation="VAAADYAGLRLPPQLRPFRYAIDRTIDRTEPPAPSITMGAAYLV
                     DNDFESNRPDSMGEFAGVPGYGAAVGLDRTTAASGESSLRVYCTESGPSFQAYMRQTP
                     FDAGRYRLVSFDYKVDEWIRVDLIVKLADGTFRTIKFTDSDSSWPALGSVANVQAKGV
                     WAHAEVPLFELMRQQIPGLKSYGVQELRIGGSGWPGNPRGASFHLDNFRILAVASGRE
                     SLAFQWYATDPAGIAGASYELNTQPDTVPDSVVDTAESRAVFTQVPEGDVYFHVRLVD
                     GAGNWGPTTHHRCFLDSAPVLASAPEPAPGEVVSTSRIAVSLQDQGLAGVDPSSIVLS
                     VAGREYPMSSPSMSYDAATGKLLWQGEKQEPEPVVFGDQQDVPVRLARLADFAGNPLA
                     TDLSWAFKMDYSKDLTPPPAPAVESSSHGCAFLEPFEGTPADGALGECSAGSGVELEI
                     DPAEKASGSGSLKVTYREGKAMRVVAHQTPFDAAQIPYLAFDYCVPAGVQIDIAFVVN
                     GSTVLVGFTDNETGAVSRLPGIIADGKWHRVEKFDISAAARQVVGNVRALNVSRVVFG
                     DFNSIETPLGSSFWIDNLTAYRPGSGPASLSWRATDPTGVDGYSYVVDDQPGTIPDEQ
                     PEGLGRQTAVEVPGGSFRFFHIRARDGAGHWGPTTHFCIAQQ"
BASE COUNT          379 a          720 c          771 g          382 t
ORIGIN      
        1 tcgtggccgc ggccgactac gcaggtcttc gactgccccc gcagttgcgg ccgttccggt
       61 atgcgattga ccgcacaatc gaccgcaccg agccgcccgc gcccagcatc accatgggtg
      121 cagcttacct cgttgacaat gacttcgagt cgaaccggcc cgactcgatg ggcgagttcg
      181 ccggggtccc gggctatggc gcggccgtgg gcctcgaccg aaccacggcc gcgagtggcg
      241 agtccagtct cagggtgtac tgcaccgagt cgggtccgtc cttccaggcc tatatgcggc
      301 aaacgccttt cgacgcgggg cggtaccggc tcgtgtcgtt cgactacaag gtggatgaat
      361 ggatccgtgt cgacctgatc gtgaagctgg cggacggcac gttccgcacg atcaagttca
      421 ccgactcgga ctcgagttgg cccgcactgg gctctgtggc caatgttcag gccaagggcg
      481 tgtgggctca tgccgaggtg ccgctgttcg agctcatgcg gcagcagatc ccggggctca
      541 agagctatgg ggtgcaggag ctgcgcattg gaggcagcgg gtggcccggc aacccgaggg
      601 gcgccagttt ccatctggac aacttccgga tcctcgccgt ggccagcggc cgggagtcgt
      661 tggcgttcca gtggtacgcg acagaccccg ccgggatcgc tggcgccagc tatgagctga
      721 acactcaacc cgacacggtg cccgacagcg tggtcgatac cgcggagagc cgtgcggtct
      781 tcacgcaggt acccgagggt gacgtgtact tccacgtcag gctggtcgat ggagccggta
      841 actggggccc gacgactcac caccggtgct tcctcgatag cgccccggtt ctggcttccg
      901 cacccgaacc ggcgccgggg gaggtggtga gcacgtcgcg gatagcggtg tcactgcagg
      961 accagggcct ggcgggagtc gacccgtcgt caatcgtgct gagtgtggcg ggtcgggaat
     1021 accccatgtc cagtccttcc atgagctacg acgcggcaac ggggaagctc ctgtggcagg
     1081 gagagaaaca ggaacccgaa ccggtcgtct tcggtgacca gcaggacgtt cccgtgcgcc
     1141 tggcccgcct cgcggatttc gcgggcaacc ccctggccac cgatctgtcg tgggccttca
     1201 agatggacta cagcaaagac ctgacgcctc cgcccgcacc cgccgtcgag tcgtcatcgc
     1261 atggttgcgc ctttttggag ccgttcgagg gcacccctgc ggacggagcc cttggggagt
     1321 gctcggcggg ctcgggggta gaactggaga tcgacccggc cgagaaggcg tcggggagcg
     1381 ggagtctgaa ggtcacctac cgcgagggta aggcgatgcg tgtcgtggct caccagacac
     1441 cattcgatgc cgctcagatc ccgtacctgg cgttcgacta ctgcgtgccc gccggagtcc
     1501 agatcgatat cgccttcgtg gtgaacggca gcaccgtact ggtagggttc accgacaacg
     1561 agactggggc agtctcccgg ctgcccggga tcatcgccga cggcaagtgg catcgggtcg
     1621 agaagttcga catctccgcc gcggcccgcc aagtggtcgg caatgtgcgc gccctgaatg
     1681 tatcgcgagt ggttttcggc gacttcaact cgatcgagac cccgctgggc tcgagcttct
     1741 ggatcgacaa cctcactgca taccggccgg gctcgggtcc cgcatccctg tcgtggcggg
     1801 caacggatcc gacgggcgtc gacggctaca gctacgttgt ggacgatcag cctggcacga
     1861 tcccagatga gcagcctgag ggcctcggcc gccagacggc ggtggaggtt cccggcggct
     1921 cgttccgctt cttccacatc cgcgctcggg atggcgccgg gcattggggt ccaacgacgc
     1981 acttctgcat cgcccagcaa taggccccgg cggagggcgc acgccgggtg agagcgaacc
     2041 gcgaccatcg aggagacgac catgccccaa cactggactc ggggagaggc gattggtatg
     2101 acgcttggcg cactgggagc gacccggctc ggcccgctgt tcgcccaaca ggcagaaggg
     2161 aacgccggga aggtccgcgt ggcgatgttg agcttcgcgc acgttcacgc caatggctat
     2221 gccgaccagg tcaaggggca ttcgcgcgcg gc
//
LOCUS       DAOSPL010000813         2252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48083_length_2252_cov_3.689577, whole genome shotgun sequence.
ACCESSION   DAOSPL010000813 DAOSPL010000000
VERSION     DAOSPL010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48083_length_2252_cov_3.689577"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..>2252)
                     /locus_tag="PLQ54_13985"
     CDS             complement(11..>2252)
                     /locus_tag="PLQ54_13985"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOS94425.1"
                     /translation="RLPGPGKTFTAQIGVDSNEQTRPGRGSVVFSVSVGGAQAFRSDV
                     MREGMAAVPVSVDLAGSRELVLEVGDAGDGIACDQSDWAEARVEMADGTTVWLGDLPM
                     LDAGQLGTSLPGPLFSFTYDGKPSAELLPTWSRDAQSEGIDDNRTRHTVTWTDPATGL
                     VVRLEAIEYLDFPTVEWTLHLSNSGDADTPILESVLPMDTGWRRSLSGEFVLHHNVGS
                     VCSASDFGPLQTPLRPNATLHVAAAGGRPTNSDMSYFNLEWAGEGRIIAVGWPGQWAS
                     DWLRDGGVGVRLKAGQELTHLKLLPGEEIRTPLMVVQTWQGGDWIRAQNIWRRWMMAH
                     SMYRPGGNLPEPAFRASSSRAYEEMIGANEENQIMHIDRYEEERLGLDTWWMDAGWYV
                     QEHGWPQVGTWEVDPARFPRGLKPISDHAHARGIEILVWFEPERVAAGTWLADNHPEW
                     ILGGAGGGLLNLGNPDAWSWLVEHVDRLINDQGIDVYRQDFNMDPLGFWRSNDAPDRQ
                     GITEIRHVEGLLAYWDELHRRHPDMLIDTCASGGRRIDLETLRRAVPLWRSDYAFEPI
                     GHQCMTYGLSMWVPYHGTGTVACSNAGYYGGGATPVEPYAFWSNAAPSLGSGIDIRVR
                     EIDWEAVRELLADWRAVNGCYYGDFYPLTPYTRDEAQWVGWQFDVPERGEGMVQVFRR
                     GRSPYEAARFPLRGLDAGKTYEVTDLATDETTRVSGEDLLGNGLLVAMPERPQAAVLR
                     YRLAGE"
BASE COUNT          367 a          802 c          716 g          367 t
ORIGIN      
        1 cgggcgccgc tcactccccg gccagccggt acctcagcac cgcagcttgg ggccgctcgg
       61 gcatcgcgac gaggagacca ttgccgagca ggtcctcgcc gcttacccgc gtcgtctcat
      121 ccgttgcgag gtcggtgacc tcgtatgtct tcccagcgtc gagcccccgc agcgggaagc
      181 gggccgcctc gtaggggctc cggccccggc ggaacacctg caccatgccc tcgcctcgct
      241 cgggaacatc gaactgccag ccgacccact gggcctcgtc ccgcgtgtac ggcgtcagcg
      301 ggtagaagtc gccgtagtag cacccgttca ctgcccgcca gtcggcgagg agctcgcgaa
      361 cggcctccca gtcgatctca cgcacgcgga tgtcgatccc tgagcccagg ctgggcgccg
      421 cgttgctcca gaaggcatag ggctctaccg gtgtagcacc tccgccgtag tagcccgcgt
      481 tggagcaggc gaccgtgccc gtgccgtgat agggcaccca catcgagagg ccgtaggtca
      541 tgcactgatg gccgatgggc tcgaacgcgt agtcgcttcg ccacagcggg actgcccgac
      601 gcagcgtctc caggtcaatg cgccgcccgc cgctggcgca agtatcgatg agcatgtccg
      661 ggtgtcggcg gtggagctcg tcccagtagg ccagcaggcc ctcaacatgc cggatctcgg
      721 tgatgccctg gcggtccggt gcgtcgttgc tccgccagaa ccccagtggg tccatgttga
      781 agtcctgccg atacacgtcg atgccctggt cgttgatcag cctgtcgacg tgctcgacga
      841 gccaactcca cgcatccggg ttgccaaggt tcagcaatcc gccgcctgcg ccgcccagga
      901 tccactccgg gtggttgtcg gcgagccagg taccggcggc cacgcgctcc ggttcgaacc
      961 agaccaggat ctcgataccc cgagcgtggg catggtcgct gatgggcttc aggccgcgcg
     1021 ggaaccgagc gggatccacc tcccaggtgc cgacctgggg ccagccatgc tcctgcacgt
     1081 accagcccgc atccatccac caggtgtcga ggccgagtct ctcctcctca tatcggtcga
     1141 tgtgcatgat ctggttctct tcgttggcgc cgatcatctc ctcgtaggcc cgggagctcg
     1201 acgcgcggaa agcgggctcg ggcagattac cgccaggacg gtacatgctg tgagccatca
     1261 tccagcgccg ccagatgttc tgtgcacgga tccaatcacc gccctgccag gtctgaacga
     1321 ccatcagtgg cgtgcgaatc tcctcacctg gcaagagctt caggtgcgtc agctcctgac
     1381 cggccttcag gcggacaccc acgccgccat cgcgcaacca gtccgaagcc cactgaccgg
     1441 gccaacccac ggcgatgata cggccctcgc cggcccactc caggttgaag taggacatgt
     1501 cggagttcgt cgggcgccca cccgctgccg cgacgtgaag cgtcgcgttt ggcctcagcg
     1561 gcgtctgcag cggaccgaag tccgacgcgc tgcacacgga gcctacgttg tgatggagaa
     1621 cgaattcccc ggatagactc ctccgccatc cggtgtccat ggggaggacg ctctcgagaa
     1681 taggcgtgtc cgcatcgccc gagttgctca agtgcagcgt ccattccacc gtcgggaagt
     1741 cgaggtactc gatggcctcc agccggacga ccagcccggt ggcggggtcg gtccaggtga
     1801 ccgtgtggcg agtcctgttg tcgtcgatcc cctcgctctg agcatcgcgc gaccaggtgg
     1861 ggagcagctc ggccgacggc tttccgtcat aggtgaacga gaacagcggg ccaggaagcg
     1921 aggtcccgag ctggcctgcg tcgagcatcg gcaggtcgcc gagccacacg gtcgtgccat
     1981 cggccatctc gacccgcgcc tcggcccagt cagactggtc gcaagcgatg ccgtcgcccg
     2041 catcgccgac ctcgagcacc agctcacggg agcccgccag atcaaccgag actggcacgg
     2101 ccgccatgcc ctcgcgcatc acgtcggagc gaaaagcctg cgcgccgcca acgctcaccg
     2161 agaagaccac gctcccgcgg ccgggccgcg tctgctcgtt gctgtcgacg ccgatctggg
     2221 cggtgaacgt cttccccggg ccgggcagcc gc
//
LOCUS       DAOSPL010000814         2251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48104_length_2251_cov_3.330146, whole genome shotgun sequence.
ACCESSION   DAOSPL010000814 DAOSPL010000000
VERSION     DAOSPL010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48104_length_2251_cov_3.330146"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLQ54_13990"
     CDS             complement(<1..329)
                     /locus_tag="PLQ54_13990"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOS94426.1"
                     /translation="MFALKPNRTKHLLSTGQCAYGTAIAEYGTPAYAQMAALAGYDYL
                     MIDREHNAWDIQTLREIVRTCRARDITPIVRIPDMVPHWISQMLDLGAEGIMIPRVES
                     RAVVEAAL"
     gene            431..1099
                     /locus_tag="PLQ54_13995"
     CDS             431..1099
                     /locus_tag="PLQ54_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOS94427.1"
                     /translation="MPSFPSLDEATTALRLAAAIGLCGVIGLERQLHRQSAGFRTHIL
                     VGVGACLVMMLSIIVGGADHDPGRIAAQVVTGVGFLGAGTIIRQRDSIRGLTTAASVW
                     CASAVGLAAGAGWFQGAAMATAGVLISLWLFKFIELHLPADDARIVVVGEACDETEAA
                     IRSVVERRRARVVSSRRHHGPDGRDWARVVIAPRRASDVDALHDALESLDRVDRVRVT
                     SSRQ"
     gene            1130..>2251
                     /locus_tag="PLQ54_14000"
     CDS             1130..>2251
                     /locus_tag="PLQ54_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94428.1"
                     /translation="MGGLRVAVAAGAAVWMLAPAPASEKGTMPRPEDFAILPWSWVPN
                     DPVQLQWIRECGFNLAGFASPDALDAVRAAGLKAFVSDGTTHVGDAEAALDDAEIARR
                     VKALVHKVRHHPAVFGYYLRDEPNAAVFPGLARWAAAYAKADPKALPYINLLPNYGSS
                     AQLGTATYEEYLDQYVRTVHPRFISYDHYALMDDGSLRGGYFQNLEAVRKAALSAGIP
                     FWNIVLANAHFRYAEPTPAGLRFQMYTTLAYGGRGISYFTYLTPDVGNYRLAPIDQFG
                     NKTPTWDMLRNVNLQIHALAPTYLTLTSVNVFHHGEVPDGCHGDETSLHLNSVDGANL
                     VVGEFTDPGGQPAVLIVNKDLHRSTAFGLEFKHPGNVMLV"
BASE COUNT          329 a          708 c          779 g          435 t
ORIGIN      
        1 agcgcggcct cgaccacggc gcggctctcg actcggggga tcatgatgcc ctcggcgccg
       61 aggtcgagca tttggctgat ccagtggggc accatgtccg ggattcggac gatgggcgtg
      121 atgtcgcgcg ctcggcaggt gcgcacgatc tcgcgaaggg tctggatgtc ccacgcgttg
      181 tgctcgcggt cgatcatcag gtagtcatat ccggccaggg cggccatctg cgcgtaggcg
      241 ggggtgccgt attcggcgat ggccgtgcca taggcgcact ggccggtgct cagtaggtgc
      301 ttggttctgt ttggtttcag tgcaaacatg gcgctcctcg tcacgtattc tgccgcgctt
      361 gggcgaccgc gcaaaggccc ggccgtggtc ggtgccgaac ctttgtgtgt ctgacctgga
      421 gggatcggta gtgccctcgt ttcccagtct ggacgaagcc accacggccc tgcggctggc
      481 ggcggcgatc ggcctgtgcg gcgtcatcgg gctcgaacgg caactgcatc gccagtccgc
      541 gggtttccgc acccatattc tggtcggtgt gggcgcgtgc ctggtgatga tgctctcgat
      601 catcgtgggc ggtgctgatc acgacccggg acgcattgcc gcccaggtgg tcaccggtgt
      661 gggttttctg ggcgccggca ccatcatccg ccagcgcgat agcatccgtg gtctgacgac
      721 agcggcgagc gtatggtgcg cgtcggcggt gggtctggcg gctggagcgg gctggttcca
      781 gggcgcggca atggcgacgg cgggggttct catttcgctc tggcttttca agttcatcga
      841 gctccacctg ccggcggatg atgcccgcat tgtggttgtc ggcgaggcct gcgatgagac
      901 ggaggccgcc attcgaagcg tggttgagcg ccgccgggcc cgggtcgtct cgagccggcg
      961 gcaccatggc cccgatgggc gcgattgggc ccgagtcgtg atcgcacccc ggcgcgcgtc
     1021 tgatgtcgac gcgcttcacg atgccctcga gtcgctggac cgcgtcgacc gggtgcgggt
     1081 gacgtcaagc cggcagtgac ccctttggtt ccgcgagaga gtgatgtgca tgggcggttt
     1141 gcgcgttgcg gttgccgcgg gagcggcggt ctggatgctg gccccggcgc cggcaagtga
     1201 gaagggcacc atgccgcggc ctgaggattt cgccatcctt ccgtggagct gggtcccgaa
     1261 tgatccggtg cagctccagt ggatccgcga gtgcgggttc aacctggcgg gtttcgcgag
     1321 tcccgatgcg ctcgatgcgg tccgcgctgc tggtctcaag gcctttgtct ccgatggcac
     1381 cacccacgtt ggcgatgctg aggcggccct ggatgacgcc gagatcgcgc ggcgcgtgaa
     1441 ggcgctggtt cacaaggttc gccaccaccc ggccgtcttc ggctactact tgcgggacga
     1501 acccaacgcc gcggtgttcc ccggcttggc gcgttgggct gcagcctacg ccaaagctga
     1561 ccccaaggcg ttgccctaca tcaatctgct tccgaactac ggctcgtcgg cgcagttggg
     1621 gacggccacg tacgaggagt acctggatca gtacgtccgg accgtacacc cgcggttcat
     1681 cagctacgac cactatgccc tgatggatga cggttcgctg cgcgggggct acttccagaa
     1741 cctggaggcg gtgcggaagg cggccctctc ggccggcata ccgttctgga acatcgtctt
     1801 ggcgaacgcg cacttccgct atgccgagcc cacgccggcg gggctgcggt tccagatgta
     1861 caccacgctc gcctatggtg gccgcgggat ctcctacttc acctacttga cgcccgatgt
     1921 gggcaactac cgcctggcac ccatcgacca gtttgggaac aagacgccca cgtgggacat
     1981 gctccggaat gtgaacctgc agatccatgc gctggcgccg acctacctca cgctgaccag
     2041 cgtgaacgtg ttccaccacg gcgaggtccc cgacggttgc cacggcgatg agacgagcct
     2101 gcatctgaac agcgtggacg gcgccaacct ggtggtgggg gagttcaccg atcctggagg
     2161 gcagcccgcc gtgctcatcg tgaacaagga ccttcatcgc tcgaccgcct tcggcctcga
     2221 gttcaagcac cccggcaatg tcatgctggt g
//
LOCUS       DAOSPL010000815         2251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48111_length_2251_cov_2.802823, whole genome shotgun sequence.
ACCESSION   DAOSPL010000815 DAOSPL010000000
VERSION     DAOSPL010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48111_length_2251_cov_2.802823"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="PLQ54_14005"
     CDS             complement(<1..1167)
                     /locus_tag="PLQ54_14005"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOS94429.1"
                     /translation="MKLRPRPARWLPRGLLAVALLMAPTPAFAQASARGISLDEAIEI
                     ALKQSPSLTVASRSIDLAKVGIETASLPNNRSIAGFVNVDSQLPLTTIEFPDPADPTK
                     TQSVSLGSPVTSTLGVSYRQPLLKNKQVRAGKRSAEETVQSAESSRETAVQDVIGNVK
                     QAYYAVLLGIELRRVAEEQLSRNRQHLRIAEARRDQGVAPELEVMQAQVDVTQAEHSL
                     RAAEGAVRQAVAALNQAMGLEVTAPTVVLPPEPAAPEVYDFEPLFDIARGTRSELSAL
                     DHQIASAEWEKRRADTLDRPDLNLTSTYSYNMGSAFLKGNSLALGLALTVPFDDGGVS
                     KATKHAAVENIRLLQAQKEALLQGIALEVESARIALQTAVDKLGPAREAVARAEE"
     gene            complement(1169..1396)
                     /locus_tag="PLQ54_14010"
     CDS             complement(1169..1396)
                     /locus_tag="PLQ54_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013152036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOS94430.1"
                     /translation="MEDLFRGAVTVGERGQVVIPAEVRRVVGLSPGDKLLVFCHPNGP
                     GVMMVKLHALHEATQFMQRTIEELEAQSEET"
     gene            complement(1617..>2251)
                     /locus_tag="PLQ54_14015"
     CDS             complement(1617..>2251)
                     /locus_tag="PLQ54_14015"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS94431.1"
                     /translation="ETFSPFDKLVELLGIEAAMLGLMDDPGRCHAVLERGLEYAANWG
                     AAQVRCGIDAIKISSPYVGGGLLSRRFYGEFVVPYERRLVERLHAAGPVRVYTHTCGA
                     IGDRLEDMVSTGIDGLECLDPPPLGNVELEDAKRRIGGRVFIKGNVDSVNTLLKKRPA
                     EIEADVRRTMAAGAPGGGFILSTACSIAPGVPPEHVQILAAVARESGPGW"
BASE COUNT          339 a          828 c          716 g          368 t
ORIGIN      
        1 ctcctcggcc ctggcgaccg cctcgcgcgc gggacccagc ttgtccacgg ccgtttgcag
       61 tgcgatccgc gccgactcga cctccagcgc gatgccctgc agcagcgcct ccttctgcgc
      121 ctgcagcagg cggatgttct ccaccgccgc atgcttcgtg gccttgctca ccccaccgtc
      181 gtcgaagggc acggtcagtg ccagaccgag ggccagcgag ttccccttca ggaacgcgct
      241 ccccatgttg taggagtagg tgctggtcag gttcagatcc ggccggtcca gggtgtccgc
      301 acgccgcttc tcccactcgg ccgaggcaat ctggtgatcc agggcgctca gctccgaacg
      361 cgtcccccgg gcgatatcga acagcggctc gaagtcatag acctcggggg ccgcaggctc
      421 gggcggcaga acgaccgtgg gggcggtgac ctcgaggccc atggcctggt tcagcgccgc
      481 caccgcctgg cgcacggccc cctcggcggc ccggagcgag tgctcggcct gcgtgacatc
      541 cacctgggcc tgcatgacct cgagttccgg ggccaccccc tggtcgcgcc gcgcctccgc
      601 gatccgcaag tgctgacgat tccggctcag ctgttcctcg gcgacccggc gcagttcgat
      661 gcctagcagc acggcatagt aggcctgctt cacgttgccg atgacatcct ggacggccgt
      721 ctcacgcgag ctctcggcgg actgcaccgt ctcctcggcc gaacgtttgc cggcccgcac
      781 ctgcttgttc ttcagcagcg gctgccggta gctcacaccg agcgtcgagg tcacggggct
      841 gcccaacgac accgactggg tcttcgtggg atccgccgga tcgggaaact cgatggtggt
      901 caggggcagc tgcgagtcca cgttcacgaa cccggcaatg gagcggttgt tgggcaggga
      961 cgccgtctcg atccccacct tggccagatc gatgctacgg ctggccacgg tcagggacgg
     1021 gctctgcttg agggcgatct cgatggcctc atccagcgag atgcctctcg cactggcctg
     1081 agcgaaggcc ggtgtcggcg ccatcagcag cgcgacagcg agaagcccgc gcggcagcca
     1141 gcgggcgggc cgggggcgca acttcatctc atgtctcctc cgattgggct tcgagctcct
     1201 cgatggtgcg ctgcatgaac tgcgtggcct cgtgcagcgc atggagcttc accatcatga
     1261 caccgggtcc gttgggatgg cagaagacca ggagcttgtc gccgggcgag agccccacga
     1321 cccggcgcac ttcggcgggg atcaccacct ggcctctctc ccccaccgtc acggcgccgc
     1381 ggaacaggtc ctccatcgtg agccgtgggt gggtcccggg gtccacttcc ggccgggcct
     1441 ctcgcgcctt gcgcgtcgac ccggcccggg ccgactgtcc cttcgccact cgcgccacct
     1501 ccccggttgc tccatgctga ctgcagagcc atggacggac cgcgtcatac tgccgtcaca
     1561 tgcggtgcgt gtcaagtgtg attattcata taagcatgat atcaacgtcc ccgcgctcac
     1621 cagcccggcc ccgactcccg cgcgacggcc gccaggatct gcacgtgctc cggaggcacc
     1681 cccggcgcga tggagcacgc cgtgctcagg atgaacccgc cgcccggggc ccccgcggcc
     1741 atggtccggc gaacgtcggc ctcgatctcg gccggacgct tcttcaggag cgtgttcacc
     1801 gagtccacgt tgcccttgat gaatacgcgg cccccgattc gccgtttcgc atcctcgagc
     1861 tccacattgc ccagcggggg gggatccagg cactcgaggc cgtcgatgcc cgtcgagacc
     1921 atgtcctcga gccgatcgcc gatggcgccg caggtgtgtg tatagacccg aaccggaccg
     1981 gccgcgtgca gccgctccac cagccgccgc tcgtagggca ccacgaactc gccgtagaac
     2041 cggcgcgaca ggagcccccc acccacgtag ggcgacgaga tcttgatggc gtcgatcccg
     2101 caccgcacct gagccgcgcc ccagttcgcc gcatactcga ggccccgctc gagcaccgcg
     2161 tgacagcggc cgggatcatc catcaggccc agcatcgccg cctcgatccc cagcagctcc
     2221 acgagcttgt cgaagggcga gaaggtctcg c
//
LOCUS       DAOSPL010000816         2247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48220_length_2247_cov_2.892336, whole genome shotgun sequence.
ACCESSION   DAOSPL010000816 DAOSPL010000000
VERSION     DAOSPL010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48220_length_2247_cov_2.892336"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLQ54_14020"
     CDS             complement(<1..373)
                     /locus_tag="PLQ54_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008745415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HOS94432.1"
                     /translation="MFEGLSEKLSATFKKLRSRGRLTEADISAALREVRMALLEADVS
                     FQVVKDFTNAIKEQALGEEVAKALSPGQQVVKIVHTELVRLLGSDATGLATAETPPTI
                     ILLAGLQGSGKTTMAGKLARMM"
     gene            929..2218
                     /locus_tag="PLQ54_14025"
     CDS             929..2218
                     /locus_tag="PLQ54_14025"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOS94433.1"
                     /translation="MRARVWMVVVTLLGLSLGDRVCAAPEWVQQPARLATVRDGLGNV
                     FAKLRAGQEVRIAYFGGSITAADGWRVKTLQWFRDRYPAATITEINAAIGGTGSDLGV
                     YRFRQDVLSKSPDLVFVEFAVNDGGAEPIQIWRAMEGIIRQAWRQDPSIDICYVYTMV
                     TDFAADYDKGLFPRATSADEMLAAYYGIPSISVAYPTAEMARAGRLWFSPKRDAEGNE
                     ISPPDGVVIWSDDNVHPRDAGHAIYAQTIQDQLATWESAARAEPHELKPPFVADNWES
                     ARLVPLAPAMLTPGWKVMSTTEGLGAAFHDRMPTMWSADAPGERIQFRFRGTAVGLYD
                     LLGPDGARAIITLDGEPRAVDRFDWFCTYHRLAPLHIASGLSDAVHEVTVEIAPEQPD
                     RTPVTDRVKDEAGYDPKRYDGTRMWVGGVLLIGELVD"
BASE COUNT          376 a          741 c          752 g          378 t
ORIGIN      
        1 ccatcatccg cgcgagcttg ccggccatgg tcgtcttgcc actgccctgc aggccggcca
       61 gcagaatgat cgtcggtggc gtctccgccg tggcaagccc cgtggcatcc gagccgagca
      121 ggcgcaccaa ctcggtgtgg acgatcttca ccacctgttg gccgggactc agtgctttgg
      181 cgacttcctc gccgagggct tgctccttga tggcgttggt gaagtccttg acgacctgga
      241 agctcacatc agcctcgagc agtgccatgc gaacctcgcg gagcgccgcg ctaatatcgg
      301 cctccgtgag ccgtccgcgg ctccgcagct tcttgaacgt ggcgctcagt ttctcactga
      361 gaccttcgaa catgccacga ccgtccggat cgtcagggat tgggcccagg ggcagatcca
      421 tcgaaccgcg ctgacgcgca gccctgcgcc tcacgcacca atgcgcgacc gcacaagcca
      481 aacagtgtaa tctaccgcgc ggcggctgtg ggcgtcaagc ttcgcgcgct cacacgagct
      541 cacaagccac agccacctct cgcatttcgc gggcccctcc cgttcgggct cgtggctgtt
      601 gggagacgtg tcgagaggcc gcgcagggga gcagcctacc cagtcgggcg gggcccgacc
      661 cggaccaggg ccctctgtgg ctgatgggga gcacgggctg ccgccaggcc gaaatgaccg
      721 tggctcacaa gctcgaaacc aacgtggcgt aggaatgtcc cgtcttgggc aatgtcgaac
      781 tgccccccgg ggcaagcatt cctcccggaa ctgaccgggt ccatgttacc ggcccggggc
      841 tcccggcatg tccgggtcgg gcgggcgcgg aggcacggtg ggccgcgcac ggcgaccgcc
      901 cggggggcgg cgaagagggg gtctccctat gcgcgcgaga gtctggatgg tggtggtcac
      961 gctgttaggt ctgagtctgg gtgacagggt ctgtgcggca cccgagtggg tccagcagcc
     1021 ggcccgtttg gccacggtac gtgacgggct cgggaatgtg ttcgccaagc tcagagccgg
     1081 ccaagaggtc cgcatcgctt atttcggcgg cagtatcacg gcggcggacg ggtggcgggt
     1141 caagacgctc caatggttcc gcgatcgcta tcccgcagcc acgatcaccg agatcaacgc
     1201 cgccattggc ggcacggggt ccgatctggg cgtctaccgc ttccggcagg acgtgctgag
     1261 caagagcccg gacttggtct tcgtcgagtt cgccgttaac gatggggggg ccgaaccgat
     1321 ccagatctgg cgcgccatgg aaggcatcat ccgccaggca tggcgacagg acccgagcat
     1381 tgacatctgc tatgtctaca ccatggtgac ggacttcgcg gcggactacg acaagggact
     1441 gttcccccgc gcgaccagcg ccgacgagat gctcgcggcc tactatggta tcccgtcgat
     1501 cagcgtcgca tatcccacgg cggagatggc tcgggctggc aggctgtggt tctcgcccaa
     1561 gagggatgcc gagggcaacg agatctcgcc ccccgatggt gtcgtcatct ggtccgatga
     1621 caacgtccac cctcgggatg cgggccacgc catctacgcg cagaccatac aggatcagct
     1681 cgccacgtgg gagtccgccg cccgggccga gccgcacgaa ctcaagccac cattcgttgc
     1741 cgacaactgg gagagcgcca ggctagtgcc cctcgctccg gccatgctca cgccggggtg
     1801 gaaggtcatg agcaccacag agggcctggg tgcggcgttc cacgaccgca tgcccaccat
     1861 gtggtctgcg gatgcgcccg gagagaggat ccagttccgg ttccgcggca ccgccgtggg
     1921 cctctatgac ctgctgggcc ctgatggcgc cagggcgatc atcaccctcg acggcgaacc
     1981 gcgtgcggtg gaccgcttcg actggttctg cacctatcat cgcttggcac cactgcacat
     2041 cgcctctggc ctctccgatg cggtccacga ggtcaccgtc gagatcgccc ccgagcagcc
     2101 cgaccggacg ccggtgacgg accgtgtgaa ggatgaggcc gggtacgacc ccaagcgcta
     2161 cgacggcacg cgtatgtggg tcggtggggt gctgctcatc ggagagctgg tcgactgaca
     2221 gcagggcagg cagtcgagtt gggtctt
//
LOCUS       DAOSPL010000817         2247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48224_length_2247_cov_2.373631, whole genome shotgun sequence.
ACCESSION   DAOSPL010000817 DAOSPL010000000
VERSION     DAOSPL010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48224_length_2247_cov_2.373631"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PLQ54_14030"
     CDS             <1..351
                     /locus_tag="PLQ54_14030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS94434.1"
                     /translation="SLAVVSGGTVYWLKSAFDESLARWSPGNVEHLEALRRWFADPEI
                     REIDMGATLPGDTKERWVHERRPVYAIHLFGRRPSCRAIRWDLAARAYLKRSPLGQAV
                     EARRARRRGSGRNL"
     gene            complement(437..958)
                     /locus_tag="PLQ54_14035"
     CDS             complement(437..958)
                     /locus_tag="PLQ54_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOS94435.1"
                     /translation="MTTPQDVRAAGIDALAEAMPVPVAEALWRAAGAYLARRRRATPF
                     DGAVSRAPSAGKVAPAAAADAAWLVLDPSRRRVTVRAQPVRLTRPQFALLLRLAGSPG
                     VVISYAELARALPFRDPSDAQRVRDQKSQLVRALRRASAPDGLPPVIETVAGRGLKWV
                     ADPRGVRIEDGGA"
     gene            complement(955..>2247)
                     /locus_tag="PLQ54_14040"
     CDS             complement(955..>2247)
                     /locus_tag="PLQ54_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94436.1"
                     /translation="VREKAECFATADTLARALPRVDDAGFLGDLERAEASAAAGLLAR
                     TAPHGVGFHNADMTFELRFLVERWAREGRLRVVCATSTLAMGVNLPMDNVLIDLGRSP
                     SEGGCVAIPPLTAADVENMGGRAGRPGPGAAGRYGRCILASTSVLERERLLAEYVESR
                     PGPALPTLTGDALAVAVGRLIGQQGPMAEAEVIRVYAGSWSCASGLCSEGDVAAAVAD
                     LRDRGLVTGDSTALRGSAALRAAARWGIPPTTAMSLAGLAADLVESMGPELEVLLGLA
                     AAGLEAVPLPFPRFGLDPGEWDRAYQGRLVAEGFEEPGDLSPLAMLSAPELALAQRVA
                     LAAADWIGEEPTAVIEQRHRALGGAVERLCRDLARLAQACAELPPEPGPAAQGVSRHL
                     RELSARLTSGAMRPRPSWRSCRWQAWGGITCATWWRWA"
BASE COUNT          330 a          916 c          725 g          276 t
ORIGIN      
        1 agtctggccg tcgtgtcggg cggcaccgtc tactggctca agtccgcatt cgatgagtcc
       61 ctcgcccgct ggtcgccggg aaacgtcgag catctcgagg ccctccggcg ctggttcgcc
      121 gaccccgaga ttcgcgagat cgacatgggc gcaacgctgc ccggtgacac caaggagcgc
      181 tgggtccatg agcgtcgccc cgtctacgcc atacatctct tcgggcggcg cccgtcatgc
      241 cgggccatcc gctgggacct ggccgcgcgg gcgtacctga agcgttcgcc acttggacag
      301 gctgtcgagg cgcgccgggc caggcgccgc ggctctggcc gcaacctgta gaccagggcg
      361 caccccgcca cccgccgtcc gcgccgagca ccggcgcccg gtgtctggtg ccgtcccgtc
      421 cgccctcgct ctctgcctac gcccctccgt cctcgatccg cacgccgcgt gggtcggcca
      481 cccacttcag ccctcgcccg gcaacggtct cgatcacggg tggcaggccg tcgggggccg
      541 acgcccgcct cagtgcccga acgagttgag acttctggtc tcgtacccgc tgcgcgtccg
      601 acggatcacg gaaaggtagc gcacgcgcca actcggcgta ggagatgacg acgcccggcg
      661 acccggcgag cctcagcagc agcgcgaact gcggacgcgt gagccgtact ggctgggcgc
      721 ggactgtcac acgccggcgc gagggatcca gtaccagcca cgcggcatcg gctgccgcgg
      781 cgggagcgac cttgccagcg ctgggcgcgc gcgagaccgc accgtcgaac ggcgtcgcgc
      841 ggcgcctccg cgccaggtag gcgcccgccg cgcgccagag cgcctctgcc acgggcacgg
      901 gcatcgcctc ggccagggcg tcgatgccgg ccgcccggac gtcctgggga gtcgtcacgc
      961 ccatcgccac caggtcgcgc aggtgatccc gccccacgcc tgccaccgac agctccgcca
     1021 gctcggacgc ggccgcattg cccccgaagt cagccgcgcc gatagctccc gcaggtgcct
     1081 cgacaccccc tgggcggcgg gccccggctc gggtggaagc tcggcgcacg cctgtgccag
     1141 gcgggcgaga tcgcggcata gccgctccac ggcaccgccc aacgcccgat gccgttgctc
     1201 gatgacggcc gtcggctcct cgccgatcca gtcagccgcc gccagggcca cacgctgcgc
     1261 cagcgccaac tcgggggcac tgagcatcgc cagtgggctc agatcgccgg gttcctcaaa
     1321 cccttcggcc accagccgcc cctggtaggc ccggtcccac tcgcccgggt ccagaccgaa
     1381 ccgcgggaac gggagcggca ccgcctcaag acccgcggcc gccagaccaa gaagcacctc
     1441 cagctcaggc cccatactct ccacgaggtc ggcggcaaga ccagccagcg acatcgcagt
     1501 cgtgggcggt atgccccagc gagccgccgc cctcagcgcc gccgagcccc gcagcgcagt
     1561 ggaatccccc gtcacaaggc cccggtcccg caggtcggcg acggctgccg caacatcccc
     1621 ctcgctgcag agccccgacg cgcaggacca gctccccgcg tagactcgga tcacttcggc
     1681 ctcggccatc gggccctgct ggccgatgag gcgtcccact gccaccgcca gagcgtcgcc
     1741 ggtcaacgtc ggcagtgccg gcccggggcg cgattccaca tactcggcca gcagccgctc
     1801 gcgctccagc acactagtcg aagccagaat gcaccggcca tagcgcccgg ccgcccccgg
     1861 tcccggccgg cccgcccgac cacccatgtt ctccacatcg gccgccgtga gcggggggat
     1921 ggccacacaa cctccctccg acgggctccg gcccaggtcg atcagcacgt tgtccatcgg
     1981 gaggttcacg cccatggcca gcgtgctcgt ggcgcacacg acgcgcaggc gtccctcccg
     2041 ggcccagcgc tccaccagaa accgcagctc gaaggtcatg tcggcgttgt ggaacccgac
     2101 accgtgcggc gccgtccgcg ccagcagccc agcggccgcc gaggcctcgg cccgctccag
     2161 gtcgcccagg aagcccgcgt cgtccacccg cggaagcgca cgcgccagcg tgtccgccgt
     2221 cgcgaaacac tccgccttct cccgaac
//
LOCUS       DAOSPL010000818         2246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48243_length_2246_cov_2.681880, whole genome shotgun sequence.
ACCESSION   DAOSPL010000818 DAOSPL010000000
VERSION     DAOSPL010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48243_length_2246_cov_2.681880"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..578
                     /locus_tag="PLQ54_14045"
     CDS             33..578
                     /locus_tag="PLQ54_14045"
                     /inference="COORDINATES: protein motif:HMM:NF018506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HOS94437.1"
                     /translation="MPRGDGRRERAGRVGLDPGRGLLTASQPGLCGLHDLEGDRVARI
                     GQAGYTIPLKIVRNHGVSFVETVFYMGIGAAVTGLFIFAACDGQFGVWWRQRPRDHAW
                     AGVSGMLWSFAVLTMTEAIEVVGLAVTWPIANLNTVFAVALGVWVFHEVDLRRHGRLL
                     SLGVAMAVVGTALLGVSRALR"
     gene            843..1367
                     /locus_tag="PLQ54_14050"
     CDS             843..1367
                     /locus_tag="PLQ54_14050"
                     /inference="COORDINATES: protein motif:HMM:NF005457.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HOS94438.1"
                     /translation="MAESRHGSEAGGTQSLSALVDEKVLATIRDVARLAREQGLAELA
                     VCEGGVEIRVELEGCAASPVAAAGPATGTRAVVRAAEPEAPAPQLDAEPGIHLVRAPM
                     VGVFYRAPMPDAPPFTKEGDHVAPGDVMGLIEAMKIFNEVQADAPGRVVEFLAKDEEF
                     VSLGQPLVKLKTGP"
     gene            1493..>2246
                     /locus_tag="PLQ54_14055"
     CDS             1493..>2246
                     /locus_tag="PLQ54_14055"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOS94439.1"
                     /translation="MSEPIRVGLLGCGTVGSGVARILTQNQEAIAVRAGGPVVLARVA
                     EVDPSKADAVDLPRSCVTTDSMAVATAEDIDIVVECIGGVGKARELVEAALAAGKSVV
                     TPNKELIAKAGHGLLQLAAARGVDLQFEGSVGGGIPIIRPMKESLAGDDLLSIAGIVN
                     GTTNYILTRMSEEGASFEDALAEAQKRGYAEQDPSADITGADAANKISILASIAFGNY
                     VDVSEVDTEGIDKMHPRDLVAAAELGYIVKLLA"
BASE COUNT          347 a          686 c          807 g          406 t
ORIGIN      
        1 gccggcagca tcctcatcgc tgggtctgcc ggatgcctcg gggtgacggg agacgcgaac
       61 gggcgggccg cgtcggtctc gatcccgggc gtggtctact cactgctagc cagcctggcc
      121 tatgcgggct acacgatctt gagggggatc gtgtagcccg cataggccag gctggctaca
      181 cgatccccct caagatcgtg cggaaccatg gagtctcttt cgtcgagacg gtcttctaca
      241 tgggcatcgg tgcggccgtc acggggctct tcatcttcgc cgcttgcgac ggccagttcg
      301 gcgtatggtg gcgtcagcga ccgcgggacc atgcctgggc gggcgtctcc ggtatgttgt
      361 ggtcattcgc ggtgctgacc atgaccgaag ccatcgaggt tgttggcctg gcggtgacct
      421 ggcccatcgc gaacctgaac acggtgttcg cggtcgctct gggagtctgg gtgttccacg
      481 aggtcgactt gaggcggcat ggccgcttgt tatccctcgg ggtggcgatg gcagtggtcg
      541 gcacggcgct gttgggggta tctcgggccc tgcggtgact cgagggagta cggacgccgt
      601 ccccctcagg caatgaggcg caagccagtc ggggcccgtc cgtgctgcgc accgtgtcac
      661 cgtgtccagt tcacttgccc tccgccaccc gggcctggta caatagtgtt tccgtgacgt
      721 gggagcccat ggcggcgatc tcacgggtgt cggctcgccg gggttcgggg ccgggtggtt
      781 ggtcctgact gctgccctga agcgggcctt ttttctgtgt caggtctccg gaggcagggc
      841 atttggctga gtccaggcat ggcagtgagg ccggggggac gcaaagcctt tcggccttgg
      901 tagacgagaa ggtgctcgcc accatccgcg atgtcgcccg gttagcccgc gaacaggggc
      961 tggctgagct cgccgtgtgc gagggtggcg tcgagatccg tgtggagctg gaaggctgcg
     1021 cggcctcccc cgtggcggcg gctggccctg cgacaggcac tcgggccgtg gttcgggcgg
     1081 ctgagccgga agcccctgct cctcagctcg atgccgaacc ggggatccac ctggtgaggg
     1141 cccccatggt cggcgtgttc taccgcgccc cgatgcccga tgcgcctccc ttcaccaaag
     1201 aaggcgacca cgtcgctccc ggcgacgtga tggggctcat cgaggccatg aagatcttca
     1261 acgaggtcca agccgatgcg ccgggccgag tcgtcgagtt cctcgccaaa gacgaggagt
     1321 tcgtgagtct gggccagcca ctggtgaagc tcaagacggg accttgatct gcgccccgga
     1381 cggggcgata gggtacccat cgaggggccg cccgtttcgg caggggccgc cccgcgcttg
     1441 gcacacgagg gagtaacgtg ttcgcttgct tccggggagg gagagacgcc gcatgagtga
     1501 gccgatccgg gtcgggttgc tgggctgcgg caccgtcggg tctggagtgg cgcggatcct
     1561 cactcagaac caggaggcca tcgccgtacg cgccggcgga ccggtggtgt tggcgcgtgt
     1621 cgccgaggtg gacccgagca aagccgacgc ggtggacctt ccgcgctcct gtgtcacgac
     1681 cgacagcatg gccgtggcca cggccgagga catcgacatc gtggtggagt gcatcggcgg
     1741 cgttggcaag gcccgggagc tggtcgaggc tgctctggca gccggaaaga gtgtagtcac
     1801 acccaataag gaattgatag cgaaggccgg tcatggactg ctccaactgg ccgccgcccg
     1861 aggggtggac ctccagttcg agggcagcgt cggtggcggg attcccatca tccgcccgat
     1921 gaaggagagc ctcgcgggcg acgatcttct gtctatcgcc gggattgtca acgggacgac
     1981 caactacatt ctgacccgca tgtcggagga gggcgcatcg tttgaggatg ccctcgccga
     2041 ggcgcagaag cgcggctatg ccgagcagga tccgtccgcc gacatcacgg gggccgacgc
     2101 ggccaacaag atcagcatct tggcctccat cgccttcggc aactacgtcg acgtctcgga
     2161 ggtcgacacg gaaggcatcg acaagatgca ccctcgcgat ctggtggccg ccgccgagtt
     2221 ggggtacatc gtgaagctgt tggcca
//
LOCUS       DAOSPL010000819         2243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48330_length_2243_cov_3.527879, whole genome shotgun sequence.
ACCESSION   DAOSPL010000819 DAOSPL010000000
VERSION     DAOSPL010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48330_length_2243_cov_3.527879"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /locus_tag="PLQ54_14060"
     CDS             <1..1635
                     /locus_tag="PLQ54_14060"
                     /inference="COORDINATES: protein motif:HMM:NF022752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOS94440.1"
                     /translation="LPAAPECGGDEALAECSKRVQALGYLCGGHDNYQDMYRDAPSWD
                     ESYIMRNPDGSIRQGGNWAGGPCWLICSRKALELARRNLPEVKRRYGFSAYFIDTTFA
                     APPYECSSTEHPLTFTDDIRYKGELCDLSRSFFPIHGSEEGQEWAIPHSSYFEGIMGG
                     VLSEPPAGTRQIPLMDLVYHDCIALYTHQGTRAGPADARYIIRHLSSGRMPVYSFGPH
                     AYFENEAIELAPAVPSVARVERGGDRRHIRVTYRWNVTGPMPPARCFVHFTDATGGIV
                     LQDDHDLAPGTEEWQPGQTVIDGPRDVEIPEGLSGGFDVRIGLLAGDRRLRLPAADDG
                     SLRVVVGQLHLSEKGVRFTPPEGRGAAADPTCFARADGGWGQALGATDRFIKNTYEFM
                     SPVNEITFTMRIVDHAFLDAGGRVERTVFADPRKPSRTLTIITNAGTEVFRTGDAVLP
                     PFGFVVESPEFVAFHALQYRGVIYEPSALFCARLVTGTAIGAPGSTVRVYHGFGSQTL
                     RLPSRAATAMAGEQRIKTRKGLAEIPVQTEAVVRFR"
     gene            complement(1571..>2243)
                     /locus_tag="PLQ54_14065"
     CDS             complement(1571..>2243)
                     /locus_tag="PLQ54_14065"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS94441.1"
                     /translation="RLSSIEPLEFSPELIDVLAAHAGRICRHLHIPLQSGSDRVLAAM
                     NRRYTAEQYASIVGELARRIPGLALGADVLTGFPGETDDDHARTCELVRTLPLAYLHV
                     FSYSPRDGTAAAYLPGPVRPDIIKRRSRELLAWSANRYATYLDSLVGTEQSVLFERQI
                     GEHVVGHTDTYAEVSVPAGEAERNDLAQVRIDGVTADRCFGHVIGSARLPLSGPGSPP
                     SPSAS"
BASE COUNT          350 a          749 c          770 g          374 t
ORIGIN      
        1 ttgccggcgg cgcccgagtg cggtggcgac gaggccctgg ccgagtgcag caagcgggtc
       61 caggctctgg ggtatctgtg cggtggccac gacaactacc aggatatgta ccgcgatgcg
      121 ccctcgtggg acgagtccta catcatgcgt aatcccgatg ggtccatccg gcaaggcggc
      181 aactgggcgg gtgggccatg ttggctcatc tgctccagga aggcgctcga actggcccgc
      241 cgcaacctgc ccgaagtcaa gcgccgctat ggcttctcag cctacttcat cgacaccacc
      301 ttcgcggccc cgccctacga gtgctcgtcc actgagcacc cgctgacatt caccgacgac
      361 atccgctaca agggcgagct gtgcgacctg agccgttcgt tcttccccat ccacgggtcc
      421 gaggaggggc aagagtgggc catcccgcac tcgagttact tcgaggggat tatgggcggg
      481 gtgctatcgg agccgccagc cggcacccgg cagataccgc taatggacct ggtgtaccac
      541 gactgcatcg ccctctacac ccaccagggt acgcgcgccg gccctgcgga cgcgcggtac
      601 atcatccgcc atctgagttc cggccggatg ccggtttaca gcttcggccc gcacgcctac
      661 ttcgagaacg aggccatcga gctggcaccg gccgtgccct cggtcgcccg ggtggagcgc
      721 gggggtgacc ggcgccacat ccgcgtcacg tatcggtgga acgtgacggg tccgatgccc
      781 ccggctcggt gtttcgtcca tttcaccgac gccacgggtg gcatcgtcct ccaagatgac
      841 cacgacctgg ctccgggcac ggaggagtgg cagcccggcc agaccgtcat cgacggcccc
      901 cgtgatgtgg agattcccga gggtctcagc ggcggcttcg acgtgcgcat cggcctgctg
      961 gccggcgacc ggcgcctccg tctccccgcc gccgacgatg ggtcgctccg ggtggtggtg
     1021 ggacagctcc atctttcgga gaagggtgtg cgcttcacac cgccggaggg gcgcggggcg
     1081 gccgccgacc ccacctgctt cgcccgggcc gatggcggtt ggggtcaggc tctcggtgcc
     1141 accgaccggt tcatcaagaa cacctacgag ttcatgtcgc cggtcaacga gatcacgttc
     1201 accatgcgga ttgtcgatca cgcgttcctc gacgccggcg gccgcgtgga gcgcaccgtc
     1261 ttcgcggacc cccggaagcc ttcgcggaca ctcaccatca tcaccaacgc aggcaccgag
     1321 gtgttccgga ctggcgacgc cgtgctcccg cccttcgggt tcgtggtcga gtcaccggag
     1381 ttcgtggcct tccacgcact gcagtaccgg ggcgtcatct atgagccctc ggcgctgttc
     1441 tgcgctcgac tggtcaccgg gacggctatt ggcgcgccgg ggagcaccgt gcgggtctac
     1501 catgggttcg ggagccagac actgaggctc ccgagccgcg cggccacggc gatggcaggt
     1561 gagcagagga tcaagacgcg gaagggcttg gcggagatcc cggtccagac agaggcagtc
     1621 gtgcgcttcc gatgacatgt ccaaagcacc ggtcggcggt cacgccgtcg attcggacct
     1681 gggcgaggtc gttccgttcg gcctcgccgg cggggacgga cacctcggca taggtgtcgg
     1741 tgtgtccgac cacatgctca ccgatctgac gttcgaagag caccgattgc tcagtcccca
     1801 cgagcgagtc gaggtaggtc gcgtaccggt tcgctgacca ggccagcagc tctctgcttc
     1861 tgcgcttgat gatgtctggc cgcactgggc cggggaggta ggcggcggcc gtcccgtcgc
     1921 gcggggagta gctgaacacg tgcaggtagg ccagcggcag ggttcggacc agttcgcacg
     1981 tgcgggcgtg gtcgtcgtcg gtctcaccgg gaaacccggt caggacgtcg gcgcccaggg
     2041 ccaggccggg tatgcgccgg gccagttcgc cgacgatcga cgcgtactgc tcggcggtgt
     2101 agcgccggtt catggccgcc aggacccgat cggaccccga ctggagcggg atatgcaggt
     2161 gccggcagat gcgcccggcg tgggcggcca gaacgtcgat cagctcgggc gagaactcga
     2221 gtggctcgat ggagctcaac cgc
//
LOCUS       DAOSPL010000820         2243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48335_length_2243_cov_3.113803, whole genome shotgun sequence.
ACCESSION   DAOSPL010000820 DAOSPL010000000
VERSION     DAOSPL010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48335_length_2243_cov_3.113803"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..316)
                     /locus_tag="PLQ54_14070"
     CDS             complement(92..316)
                     /locus_tag="PLQ54_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94442.1"
                     /translation="MSTTRPRLGEVLVKNGVITGDQLDEALAIQAERGGKIGPILEDL
                     FGVRAEDIADARVDQAWPELTETDNGSGSA"
     gene            524..1432
                     /locus_tag="PLQ54_14075"
     CDS             524..1432
                     /locus_tag="PLQ54_14075"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOS94443.1"
                     /translation="MAFDVVGVGASALDYLCVVPSPPKPDEKMRMEDFSVQGGGPAAT
                     AMVTAARLGLSACLFARTGSDSMGHFQLAELEREGVDVSYVSQVEGASSVFAFVMVDR
                     KSGYRSIVYNGGTLPPLAESDLRQDVIRRAGCLVVDPYELGAATVASAWAREASIPVI
                     LDADAVVPGVSQVVPLCDYVIGPAEFATAFAETRSLKRAVEFLLSYGPSVVIITMGEK
                     GCQCFTRDERFSVPAYKVDVVDTTGAGDVFHGACALGVVRGWDLRRIVEFASATAALK
                     CTSLGGRAGIPRLAEVERFMLHQRPG"
     gene            1472..>2243
                     /locus_tag="PLQ54_14080"
     CDS             1472..>2243
                     /locus_tag="PLQ54_14080"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOS94444.1"
                     /translation="MAVRHRLRVPILYRYLAAEMMLPVVAWLFGLVVVLDGNFLFILL
                     RLAGGRDLPWDATIQHLVYRIPYAIFMGIPMAYLFGTALCLNRFGRDGELTAMRMGGL
                     TPRRILAPSIIASVFWALAAFVVSDRIMPQTQHKADSGLRSILMGNITLDTTRDVLVK
                     VPQSDTMLYVRHADLPSNTLRTVVLARAFDPHHREFTVAESAVFVDDMWRLNGGRRYV
                     FDRDGRVIQHGPVQTIDIDLRAIITNYWEDYRSPEEQST"
BASE COUNT          344 a          735 c          727 g          437 t
ORIGIN      
        1 ccgacgatgg ccacccgccc gagcgacgat gcgagcgggt ggccatcccc catgccacgg
       61 cgaccgcgcg gcgttccacg gccatcccgc ctcaggcgga cccgcttccg ttatctgtct
      121 cggtgagctc tggccatgcc tggtcaaccc gggcatcggc aatgtcctcg gcccggactc
      181 caaacaggtc ttcgaggatc gggccgatct tgccaccccg ctcggcctgg atcgccaggg
      241 cctcgtcgag ctggtcaccg gtgatgacgc cattcttcac cagcacttcg cccagtcgtg
      301 gacgcgtggt ggacacgcga ctcccccctc ttgtgagtcg gtactgctcc tcagacaacg
      361 agaggcctcg cttcattgtt ccgacgactc tgtgcccccg gaccgacggc tcagccgggc
      421 cgattcctgt ggatgagagg agttgacctg gacggcagcc gaacatggtt ccgtgaacgg
      481 ttcgaccggc gctgccggtg ttgtctgtct ctgaggtgcg accatggcgt ttgatgtggt
      541 gggcgttggg gccagcgccc tggactattt gtgtgtggtg cccagtcccc ccaagcccga
      601 cgagaagatg cgcatggagg acttcagcgt ccagggcggc gggcctgcgg ccaccgccat
      661 ggtcacggcg gcgcgcctcg gcctgtctgc ctgcctcttc gcccgaacgg gcagcgacag
      721 catggggcac ttccagctcg ccgaactgga gcgagaggga gtcgatgtca gctacgtctc
      781 ccaggtcgaa ggtgccagca gcgtcttcgc cttcgtgatg gtcgatcgca agtcgggcta
      841 ccgctccata gtctacaacg gtgggacgct gccgccgttg gccgaatccg acctgcggca
      901 ggatgtgatc cggcgggcgg gctgcttggt cgtggatccc tacgagctcg gcgcggccac
      961 ggtggcctcg gcctgggcgc gtgaagcctc aatcccagtg atcctcgatg ccgatgccgt
     1021 ggtgccgggc gtcagccagg tggtgccgct gtgcgactat gtgattggcc cggccgagtt
     1081 cgccacggcc ttcgcagaga cacggagcct caagcgcgcc gtcgagtttc ttctcagcta
     1141 tggccccagt gtggtgatca tcacgatggg ggagaaggga tgccagtgtt tcacgcgcga
     1201 tgagaggttc tcggttccgg cgtacaaggt cgatgtcgtc gacacaacgg gggccggtga
     1261 cgtcttccat ggtgcctgcg ccctgggcgt tgtgcgcggc tgggatctcc gccgcatcgt
     1321 tgagttcgcg tcggccacgg ccgccctcaa gtgcacctcg ctcggtggcc gggcgggaat
     1381 ccccaggctg gcggaagtgg agcgcttcat gctccatcag cgccccggat agggtgcacc
     1441 cggtgaatac gcgcacggac cggtgcgtca catggctgtg agacaccgcc tccgggttcc
     1501 catcctttat cgctatctgg ccgccgagat gatgctccct gtcgtggcgt ggctgtttgg
     1561 gctcgtggtc gtgctcgacg ggaacttcct gttcattctc ctccgcctgg ccggcggccg
     1621 ggatctcccc tgggacgcca cgatccagca cctggtgtac cgcataccct atgccatctt
     1681 catgggcatc ccgatggcgt atctgttcgg taccgcactg tgcctcaacc ggttcggtcg
     1741 tgatggcgag ctcaccgcca tgcggatggg cggcctgacg cccagacgca tcctggcccc
     1801 ttcgatcatc gccagcgtgt tctgggctct ggcggccttc gtggtcagtg atcggatcat
     1861 gccccagacc cagcacaagg cggactcagg cttgagaagt atcctgatgg gcaacatcac
     1921 cctcgatacc acgcgtgacg tgctggtcaa ggtacctcag agcgatacga tgctctatgt
     1981 gcgtcacgcc gatctgccca gcaacacgct ccgcaccgtt gtcctggcaa gggcatttga
     2041 cccacaccat cgcgagttca cggtggccga gtcggcggtc ttcgtggacg acatgtggcg
     2101 gctcaatggc ggccgccggt atgtgttcga ccgcgacggc cgcgtcatcc agcacgggcc
     2161 ggtgcagacc atcgatatcg acctgcgcgc catcattacg aactattggg aggactaccg
     2221 gagccccgag gagcagtcga caa
//
LOCUS       DAOSPL010000821         2243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48337_length_2243_cov_2.983547, whole genome shotgun sequence.
ACCESSION   DAOSPL010000821 DAOSPL010000000
VERSION     DAOSPL010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48337_length_2243_cov_2.983547"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="PLQ54_14085"
     CDS             complement(<1..1044)
                     /locus_tag="PLQ54_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOS94445.1"
                     /translation="MNRSLLAPLALALCAPARCEDDAMARWLPGAGPLDPDGDLGARM
                     VEGIGRYLDGLNAEAPAGRAAFWRRDTSGAAAYDLSVEPNRVRLRHAIGLADPRVEPV
                     DLELVGTSERDALLATGEAYDVLAVRWPSIEGVHGEGLWLRPQGGPRSRVVAIPDADR
                     TPEMLIGLAPGVPPEAQFARRLVESGCEVLVLSLLDRADAESGNPLIRMTNQPHREFI
                     YRMAFELGRHTIGYEVQKVLAAVDWFARRPTAAPIAVAGYGEGGLLALYSAALDRRID
                     AAFVSGYFGPREGLWQEPIYRNVFGLLREFGDADLASLIAPRALVVEACLGPEVEGPP
                     TPRDGRSGAAPGRL"
     gene            complement(1370..2071)
                     /locus_tag="PLQ54_14090"
     CDS             complement(1370..2071)
                     /locus_tag="PLQ54_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94446.1"
                     /translation="MRASTQAARGGVGRRVRTAILVVAALSAACALGQQAAREGAAPA
                     AGPVAVDDGPPKAYVMSFRVVFITPTPECDPRLLYEFVEGAMRDGAVVPKDAEGFYSI
                     LALDELYRLGRDDLQVAVGPVYSVSMPANADYVGYTGVCRDGERSWGISARVEPVTQD
                     GLTAKLWMKLDMANFEGSGTAADAGQIWLDDVVLEVPRILTQETNTSEAGPKGPGKQF
                     SKASVLLVMLQEALP"
BASE COUNT          353 a          817 c          764 g          309 t
ORIGIN      
        1 cagccggcca ggcgcggcac ccgagcggcc gtcgcgcggt gtcggcggac cctcgacctc
       61 ggggccgagg caggcttcga ccaccaacgc gcgcggcgcg atcaggctgg cgaggtcggc
      121 atcgccgaac tcgcgcagca gcccgaacac gttgcggtag atgggctcct gccacaggcc
      181 ctcgcgcggc ccgaagtacc cgctgacgaa ggcggcgtcg atgcggcgat cgagcgccgc
      241 tgagtagagg gcgagcagcc cgccttcgcc gtagcccgcg acagcgatgg gcgccgcggt
      301 gggccggcgg gcgaaccagt cgacggcggc cagcaccttc tgcacctcgt agccaatggt
      361 gtgccggccc agctcgaaag ccatacggta gatgaactcg cgatgcggct ggttggtcat
      421 ccggatcagc ggattgcccg actcggcatc ggcgcgatcg agcagtgaca ggacaagcac
      481 ctcgcagccg ctctccacca gtcgccgggc gaactgggcc tccggtggca ctcccggcgc
      541 caggccgatg agcatctcag gcgtccggtc ggcatctggg atggcaacca cgcgggagcg
      601 cggtccgcct tggggtctga gccacaggcc ttcgccgtgg acgccctcga tggacggcca
      661 ccgcacggcc aacacgtcgt aggcctcacc cgtcgcgagc agagcatcgc gctcggatgt
      721 gccaacgagc tccaggtcca ccggctccac ccgagggtcg gcgagcccga tggcgtggcg
      781 cagccggacg cggttcggct ccacggatag gtcataggcc gccgcgccgg acgtgtcccg
      841 tcgccagaac gccgcgcggc cggccggcgc ctccgcattc aggccatcga ggtaccgccc
      901 aatgccctca accatccggg caccgaggtc accgtctggg tcgagcggac ccgccccggg
      961 caaccatcga gccatggcgt cgtcctcaca gcgcgcgggg gcacagaggg cgagggcgag
     1021 cggcgcgagc agggaccggt tcatgcgagc cacctccggc gaggattgcg cggcgcagtt
     1081 cgccgccgga gcacgcgcgg acctgcgcgc gcttcccgat agccgacgtc ccgggcgcgc
     1141 caaccgtgga ccccattcgg ctaccgcccg actggcgtcg gtcgcacaag agcacaggcc
     1201 cgcccgcgca ggccgagtag cggtgaccgg cagatccccc gggccccccg gcggggtgga
     1261 ggctgggcca gtccggtggc aggagcacgc cggggacggc cacccgcccc aggaaaggaa
     1321 gtgagtagcc gtccccggtc gcgagcacct cacgggaccc ggccgagccc tacggcagcg
     1381 cctcctgcag catcacgagc agaacgctgg ccttgctgaa ttgcttgccg gggcctttcg
     1441 ggcctgcttc gctcgtgttg gtctcctgcg tcaggatccg tggcacttcg agcactacgt
     1501 cgtcgagcca gatctgccca gcgtccgcgg cggtgccgct gccttcgaag ttggccatgt
     1561 cgagcttcat ccacagcttg gcagtgaggc cgtcttgcgt gaccggctcg acgcgggcag
     1621 agatgcccca cgagcgctcc ccatcacggc agacgcccgt gtatccgacg tagtcagcat
     1681 tcgcgggcat cgacacgctg tagactggcc caacagccac ctgcaggtcg tctcgcccga
     1741 ggcggtacag ctcgtccagc gccaggatgc tgtagaagcc ctctgcgtcc ttcggcacaa
     1801 cagcgccgtc cctcatagcc ccttccacga actcgtagag cagccgcggg tcgcactccg
     1861 gtgtgggcgt gatgaagacg accctgaagc tcattacgta ggcctttggc gggccatcat
     1921 ccaccgcaac gggacccgcc gcgggggctg cgccctcgcg ggccgcctgc tgaccaagcg
     1981 cgcaggccgc cgagagggcg gccacaacca ggatggcagt cctgacccgc cgacctaccc
     2041 cgccccgcgc agcctgagta gaagcccgca tcgtcgtcct ccctacacgg atctctcacg
     2101 ccgcttgccg ctatggcgac gggaccgtga tctggctggt accgctgttc gactcccagg
     2161 tggtgtagtc accctcgtgc gaccagagct gcgggtgggg accgcccggt accattggcg
     2221 gtagctgagg gctccagtgg ccc
//
LOCUS       DAOSPL010000822         2243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48340_length_2243_cov_2.858775, whole genome shotgun sequence.
ACCESSION   DAOSPL010000822 DAOSPL010000000
VERSION     DAOSPL010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48340_length_2243_cov_2.858775"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..563)
                     /locus_tag="PLQ54_14095"
     CDS             complement(54..563)
                     /locus_tag="PLQ54_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94447.1"
                     /translation="MPVALALAGGACSAQVVPPTVVSVSSKVFTPMSGSYVAGQMVTG
                     ATEHRAVFNTLQADFLPCRLRLVFSQYPSWAQSAAYGDTQDHYTYVRHATVPMFAQPN
                     PRLKTWYIPNEQGPKSYAFVATTIVIPASCSQTYGPPPPPVPAEKDRPYTEGVAVEGS
                     ADVTQINVP"
     gene            1399..1650
                     /locus_tag="PLQ54_14100"
     CDS             1399..1650
                     /locus_tag="PLQ54_14100"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOS94448.1"
                     /translation="MASARVSSKGQITLPAALRRKFGIGPSSTVEVEGREGEIVIRPL
                     RSVSEVRGAFRHCVADGPMDWSAAREQAQRTVAEQVAHE"
     gene            1643..2068
                     /locus_tag="PLQ54_14105"
     CDS             1643..2068
                     /locus_tag="PLQ54_14105"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOS94449.1"
                     /translation="MSDPVPVSVVDANVLLRFLLDDHPELSGKAAAILERMERGGEVL
                     HCDPVTLAEVVWVLGSFYKQPNDAIARALEPILKAEGLRMPEKARYIRALGLFAHTVA
                     SFGDACACAAALEDCQGRLYSFDRKLSEVDGVDRRERPD"
BASE COUNT          371 a          712 c          784 g          376 t
ORIGIN      
        1 gcgtcaccgc cggtgcccac gatgtcgatg gcgcgcaggc ccaccggcag cagctaaggc
       61 acgttgatct gcgtcacgtc cgcgcttccc tccacggcaa cgccctccgt gtatgggcgg
      121 tccttctcag ccggcaccgg cgggggaggc gggccgtacg tctgactgca cgacgctggg
      181 atgacgatag ttgtagcgac gaacgcgtac gactttggac cctgctcatt gggtatgtac
      241 caagtcttca ggcgcgggtt cggctgggca aacatcggca cggtcgcatg gcgcacgtac
      301 gtgtagtgat cctgcgtgtc gccataggcc gcgctttgcg cccacgaggg gtactgggag
      361 aagacgagcc gcagacggca gggcaggaaa tcggcctgta gcgtattgaa cacagcccta
      421 tgctctgtcg ccccggtgac catctggcct gccacatagc tgccgctcat tggggtgaag
      481 accttggacg agacggacac gaccgttggg ggcacgacct gcgcggaaca ggcgccaccg
      541 gccagcgcca gggctaccgg caacgcaatg aagctcagac gattcgatag tgagtgacgc
      601 tctctctctc tctcttcatt cgactccatc cttgcacgac aggaacagca cggcctgcca
      661 tcgaccaacg atgaccacta tatgatatct agtctacaga tgcagtcaag tacctcactc
      721 gtgatttccg tcggcgcgaa gtagcagtgt ccgatcgtcg cctagtagaa atgccctgtg
      781 gaccgatgac agggcatgga gccgtatgcg cgagcccagt gcggcgtcag ttgcggtggc
      841 aagcggcggc gggggagggg accctccgtc gttggcgtga ggaaccccgg accaggtgcc
      901 gacgcgggct tcgggagcgc aggcttcagc cggcactggt ggggagcgac gacgcaccca
      961 ggccccgacc gcccgccggc ggatttgcgc aggagctgta cggaacgggc gaacagacaa
     1021 cccatgaatg gctcaccgga tcgtcgtgtc gtgcagcagg gaatcgcatt tggcgccggc
     1081 cctggcgtcg cgttaggagc cggggtcgga gtcgcgatgc accggatcgc catcgggatg
     1141 ggcctcggcg ccgtcttcgg cgtcctcatg gctctgcgct acatcaagag gcactcggga
     1201 ccgaatgggg gatcgtagcg gggccgcgtt cgcggcagcg ccgccagcga cgaggggtct
     1261 tggagcgcgg tgggacgctg cccttgccct ccttcgccac gcagacaccc ggtcggaggc
     1321 ccgacttcga gtcgggcgcg aggggccgtg gcaccgccgc ttggcacggt gtagaatacc
     1381 aagcgggagg tgacatgcat ggcatctgcc cgtgtatcca gcaaaggtca gatcacccta
     1441 cctgcggccc tgcgtcggaa gttcggaatc ggcccgtcgt cgacagtcga ggtggagggg
     1501 cgcgaaggcg agatcgtcat tcgcccgctc cggtcagtgt ccgaggtccg gggcgcattc
     1561 cgacactgtg tcgccgacgg cccgatggac tggagcgcgg cgcgcgaaca ggcgcagcgt
     1621 acggtggccg agcaggtggc ccatgagtga tccggtgccc gtgtccgtcg tcgatgccaa
     1681 cgtgctgctc cggttcctgc tggacgacca ccccgagctc tcgggcaagg cggccgccat
     1741 tctcgagcgc atggagcgcg gaggtgaggt gttgcactgc gacccggtca cactggcgga
     1801 ggtcgtgtgg gtgctgggga gcttctacaa gcagcccaac gacgccattg cgcgggcgct
     1861 ggagccgatc ctcaaagccg agggactgcg catgcctgag aaggcccgct acatccgggc
     1921 gttggggctg ttcgcgcaca cagtggcgag cttcggcgat gcctgcgcct gcgcggcggc
     1981 cctcgaggac tgccaggggc ggctctattc gttcgaccgc aagctgtcgg aggtggatgg
     2041 cgtggaccgc cgcgagcggc cggactgacc ctgcgtgcct cgggccgcct accttcctcc
     2101 cgccagctcc ctgcacacgc cctcgacgta ggcgcggtaa tccgtcgccg gcatgttggc
     2161 gaggcgggtc agcatgtcgt cggggctgat gagcccctgc cggagcgcgg cttcctcggg
     2221 gcagccgatc ttcacgctct ggc
//
LOCUS       DAOSPL010000823         2241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48399_length_2241_cov_3.223239, whole genome shotgun sequence.
ACCESSION   DAOSPL010000823 DAOSPL010000000
VERSION     DAOSPL010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48399_length_2241_cov_3.223239"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1177)
                     /locus_tag="PLQ54_14110"
     CDS             complement(119..1177)
                     /locus_tag="PLQ54_14110"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOS94450.1"
                     /translation="MKGTPLFLPGDRVGSWVGALAGSSTVFGPQVGSSGRPEFALVRD
                     PSAVDLSLDARRASIRELWMPQTEILFRYRLDSQGATLEAVPRPEGSRVAFGIRPCEA
                     AAIRRVTHVFRQGKHPDPLGLEAEGMAIVGLYCASPRRTCFCAQVGSPVDMAEEACDV
                     LMTPISGGHVLHVMTDRGGSLAGVSGAAQATSDQVSAAGAVHAGLITAVPADGAIGAL
                     EATALEVWASEVFADQGARCIGCGVCTFLCPACHCFDILDERQGTGGARYRCWDSCQF
                     DHFTLHASRHNPRHDQAARLRQRVLHKFLYMPQEFGVTGCTGCGRCAELCPVGISIRQ
                     VVADVRAAMAQEQGVTVA"
     gene            complement(1174..2160)
                     /locus_tag="PLQ54_14115"
     CDS             complement(1174..2160)
                     /locus_tag="PLQ54_14115"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOS94451.1"
                     /translation="MSDPDPVPTLRAKARELLEQGTVRAVVGYQSHAESGLPVPAVIT
                     RPDQAEALVFNPLCAHNLSRYVQGGAEKPVAVVAKACDTRALVVMMQESHIRREDVFV
                     IAMGCTGIVDAARIDERARAPWRRIEAIRTDGDAVAVETDAGVARLSLTEDLLPRCVA
                     CPVPQPVIDDVRLGDFPEAPPGRPDPLAAQVAEIEAMGRDERRRFFSETYNRCIRCYA
                     CRNVCPLCFCEKCFADETRPQWVPRSTLGGGNELYHMVRAMHLAGRCIGCGACERACP
                     VGLPLMAVNTKMAQVVDGMFGHVAGVRMDDTPPFAVYRDADPDLPGHGGGKS"
BASE COUNT          408 a          840 c          688 g          305 t
ORIGIN      
        1 ccctcgcgca gagcctcgtc ctcaaagacc atgcggaaag tcttgatgtc ataggtctcg
       61 tcgaacacct ggtcgatccg cgccaaggcg ggcaaatacg gattgcgggc gggctcggtc
      121 acgccacggt caccccttgc tcctgtgcca tggcggcccg gacatcggcc accacctggc
      181 ggatcgagat ccccaccggg cagagctcgg cgcaccgccc gcaacccgtg cagccggtca
      241 ccccgaactc ctggggcatg tagaggaact tgtgcaggac gcgctggcgc agtcgcgccg
      301 cctggtcgtg tctcgggttg tgccgcgacg cgtgcagcgt gaaatggtca aactgacacg
      361 aatcccaaca ccgatagcgc gcgccgcccg tgccctgcct ctcgtccagg atgtcgaaac
      421 agtggcaggc aggacagagg aacgtgcaca ccccacagcc gatgcaacgc gcgccctggt
      481 ccgcgaacac ctcgctcgcc cagacctcga gcgccgtcgc ctcgagagcc ccgatggccc
      541 catccgccgg cacggccgtg atgagccccg catgcacggc cccggccgcc gaaacctggt
      601 cggatgtggc ctgggccgca cccgaaacgc ccgccaatga cccgccgcgg tcggtcatca
      661 cgtgcagcac atggccgccc gatatgggcg tcatcaacac atcacaagcc tcttcggcca
      721 tgtcgacagg cgaccccacc tgggcacaaa agcacgtccg ccgcggcgag gcgcaataga
      781 gaccaacgat ggccatgccc tcggcctcga gccccagcgg gtcgggatgc ttgccctggc
      841 ggaacacgtg cgtcacgcga cggatcgccg cggcctcaca aggccgaatg ccgaaggcga
      901 cccgtgagcc ttccggcctc ggaaccgcct cgagcgtcgc gccctggctg tccagccgat
      961 agcggaacag gatctccgtc tgcggcatcc agagctcgcg aatggacgcg cgccgcgcat
     1021 ccagtgacag gtctacggcc gacggatcac gcaccagggc gaactcgggc ctccccgagg
     1081 aaccgacctg cgggccgaac accgtcgatg aacccgccag cgcaccgacc cagctcccga
     1141 cgcggtcgcc cggcaggaac agaggcgtgc ccttcacgac ttcccacctc cgtgccccgg
     1201 cagatccggg tccgcatcgc ggtaaacggc gaaaggcggc gtgtcatcca tgcgcacacc
     1261 ggccacgtgg ccgaacatgc cgtcaaccac ctgggccatc ttggtattga ccgccatcag
     1321 agggaggccc actgggcacg cgcgctcaca cgcaccgcag ccaatgcacc ggccggcaag
     1381 atgcatggcc cggaccatgt ggtacagctc attgccgccc ccaagtgtcg aacgaggcac
     1441 ccactggggg cgcgtctcat cggcgaagca cttctcgcaa aagcacaacg ggcacacgtt
     1501 acggcatgca tagcagcgga tgcagcggtt gtaggtctcc gagaagaacc ggcgacgctc
     1561 gtcccgaccc atggcctcaa tctccgccac ctgagccgcc aggggatcgg ggcgaccagg
     1621 gggcgcctcc gggaagtcgc ccagtcgcac atcatcgatc acgggctgcg gaacggggca
     1681 ggccacgcat cggggcagca ggtcctccgt caggctcagc cgagccacac ccgcatcagt
     1741 ctcgaccgcc acggcatcgc cgtcggtgcg gatggcctcg atccggcgcc acggcgcccg
     1801 ggccctctcg tcgatgcgcg ccgcatcgac gatgcccgtg caccccatgg caatcacgaa
     1861 cacgtcctcg cgccggatgt ggctctcctg catcatcacc accagggccc gggtatcaca
     1921 ggccttggcg accacggcca cgggcttctc cgccccgccc tggacgtacc gcgacaggtt
     1981 gtgggcacaa agcgggttga acaccagcgc ttccgcctgg tccgggcggg tgatcaccgc
     2041 gggaaccggc agtccgctct cggcatggga ctggtacccc acgaccgcac gaaccgtccc
     2101 ttgctccaac aactcccgcg ccttggcccg gagggtgggg accgggtccg gatcagacat
     2161 gcgccatcgc cgccttctgc tcggccgcgt tcagggcctc aaccatctca cggatgacct
     2221 gcgcgaactc cttgccctca g
//
LOCUS       DAOSPL010000824         2241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48410_length_2241_cov_2.391583, whole genome shotgun sequence.
ACCESSION   DAOSPL010000824 DAOSPL010000000
VERSION     DAOSPL010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48410_length_2241_cov_2.391583"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1882
                     /locus_tag="PLQ54_14120"
     CDS             <1..1882
                     /locus_tag="PLQ54_14120"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HOS94452.1"
                     /translation="EVGQRMITDAFAYLAAASAGGKLKLPGDLLTGRRYEIDFVDADS
                     VVAGDSAGAALAVAAYSVLTNTPVLDDVAVTGAVTRDGLVKSVGDIATKIDGVIRSHT
                     GTMILPYENAKTTPEAMAEVVRKAFPRVRVVFATTMEQVFFHAFGPYGPDGDRYRAYS
                     AEYGKGVQQLTDRQFEDAAATFSALAEQYPEDNTAVIWLANVKRAHLDDLVARARREL
                     SFDDRRRAREFVLKAFEIWPDAPEVTELLRALSGEASEPSATPESVAPAAVRPETGSV
                     APGEGPVVAVWQARAPTEPTDAAWRAIGCRRIPFEAGDAGAPELQARIAAAYTESALC
                     LLLAIRDGTEDREPPRWELGESGFHLAGGDDRFFVVWDQDIRTGADLFTRSPAMVGLS
                     SRIEWGQGDVWHWRAGATDRQRCADDLYIGPEGLRGDVGRPPYVANLNKEATGPAFVA
                     DGRVGRYGEGVLSDQSVAELRGNPLGGRETFHQRCIRCHGPDAPADQAGVTTGLAVRE
                     GGSQQALRSKLEEAAHAQALTAVSPEDVIAYISVIPSVPGVICLDPSGSRASVRAASA
                     YADGWWYVVLLRRLTTGAPDDLALHADGTYGLYVQVVDGTTGSEWRSRRVELAFGASG
                     VQ"
     gene            2131..>2241
                     /locus_tag="PLQ54_14125"
     CDS             2131..>2241
                     /locus_tag="PLQ54_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HOS94453.1"
                     /translation="MSIKVGINGFGRIGRQTFKAIQQKYADRIDVVAVNDL"
BASE COUNT          375 a          663 c          825 g          378 t
ORIGIN      
        1 cgaggtgggc cagcgcatga ttactgatgc gttcgcgtac ctggcggcgg cttcggccgg
       61 gggcaagctc aaactgccgg gcgacctgct gaccggccgg cgctatgaga tcgactttgt
      121 ggatgcggac tcggtggtgg ctggcgattc ggcgggcgcg gcgcttgcgg tggcggccta
      181 ctcagtgctc acgaatacgc cagtcctgga cgatgtggcg gtgaccgggg ccgtcacacg
      241 cgacggcctg gtgaagagcg tgggcgacat cgcgaccaag atcgatggcg tcatccgctc
      301 gcacacgggc acgatgatcc tgccctacga gaacgccaag acgactcctg aagccatggc
      361 cgaggttgtc cggaaggctt ttccgagggt tcgggtggtc ttcgcgacca ccatggagca
      421 ggtgttcttc cacgcctttg gcccctacgg ccccgacggc gatcggtacc gcgcctactc
      481 ggccgagtat ggcaaggggg tccagcaact gacggatcgg cagtttgagg atgccgccgc
      541 gactttctcg gctttggcgg agcagtaccc ggaggacaac acggcggtca tttggctggc
      601 gaacgtgaag cgggcccacc tggatgacct ggtcgcacgc gcgcggcgcg agctcagctt
      661 cgacgaccgg cggcgcgcgc gggagttcgt gctgaaggcc ttcgagatct ggccggacgc
      721 gcccgaggtg acggagctgc ttcgggcgct ttcgggcgaa gccagcgaac cgtcggcaac
      781 gccggagagc gtcgccccgg ctgcggtgcg cccggagacg ggcagcgtgg cgcctggcga
      841 gggcccggtg gtggccgtat ggcaggccag ggcccccacc gagcccacgg acgcggcgtg
      901 gcgcgccatt gggtgccgaa ggatcccctt cgaggccggt gatgccggcg cgccggagct
      961 ccaagcccgc atagccgcgg catacacaga gagcgcgctc tgcctgctgc tggcgatccg
     1021 cgatggaacg gaagatcgcg agccgccgcg atgggagctg ggggagtctg gcttccacct
     1081 cgcgggcggc gatgaccgct tctttgtggt gtgggaccaa gacatacgca ctggcgccga
     1141 cctgttcaca aggtctccgg ctatggtcgg gctgtcatcc aggatcgagt ggggacaggg
     1201 ggatgtttgg cactggcggg ccggtgccac cgaccgccaa cgttgcgccg acgacctgta
     1261 catcggcccg gagggcctcc gcggcgatgt gggacgtcca ccgtatgtgg cgaatctgaa
     1321 taaggaagca acgggtccgg cctttgtggc tgatgggcgt gtgggccgtt acggggaagg
     1381 ggttctctcg gaccagagcg tggcggagct gcgcggtaac ccactagggg ggcgcgagac
     1441 attccaccag cgatgtatcc gctgccacgg tcccgacgcg ccggcggatc aggcgggcgt
     1501 cacgacgggg ctggcggtcc gtgaaggcgg gtctcagcaa gccctgagga gcaagctgga
     1561 ggaggccgct cacgcccaag ctctgacggc ggtctcgcca gaggatgtga ttgcctatat
     1621 ttcagtaatt ccttcagttc ccggggtcat ctgtttggat ccgagtggca gtcgggcgag
     1681 tgtccgtgcc gcgtcggcct acgcggacgg gtggtggtat gtggtgctgc tccggcgcct
     1741 gaccaccggc gcgccggacg atctggcgct gcacgccgac gggacatatg ggctatatgt
     1801 ccaggtggtc gatgggacca ccgggagcga gtggcgcagc cgcagggtcg agctggcgtt
     1861 cggggcgtcg ggagtgcagt agcgccgaag agcagaacgg ctcaggtgaa gagtcgtgct
     1921 gtatacatta ccaattgcac atgatccaca gctgaatagg tgtggtgtgg ctgccgcggc
     1981 tgattgacaa aggccggagg ggcgcgctag tattcgccga acttgcgcga atgcagcaca
     2041 cagcgccgcc gggagcgcgc gctggggcgc gctgcacgcc gctgaccccg gcccgcaccc
     2101 atcccgtatg ccagatggag gagataagaa ttgtccatta aggtcggaat taacggtttt
     2161 ggccgtatcg gccgccagac cttcaaagcg attcagcaga agtatgccga ccgcatcgac
     2221 gtggttgcgg taaacgatct g
//
LOCUS       DAOSPL010000825         2240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48426_length_2240_cov_3.776659, whole genome shotgun sequence.
ACCESSION   DAOSPL010000825 DAOSPL010000000
VERSION     DAOSPL010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48426_length_2240_cov_3.776659"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLQ54_14130"
     CDS             complement(<1..217)
                     /locus_tag="PLQ54_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94454.1"
                     /translation="MRPIPEPHDSDELRIHQVRRERYEDEASVLGPVLLRLRQQGKSG
                     PVCRLAERVLQLPMLSAAPHREQRLRVH"
     gene            746..1246
                     /locus_tag="PLQ54_14135"
     CDS             746..1246
                     /locus_tag="PLQ54_14135"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOS94455.1"
                     /translation="MQCKTAINQELPERQVRSRGRRRRAPTPRPPALEIPLDRVFLYH
                     EALSALPTGNVDEFALLKRSIESIGQLVPILVWDGVLIDGRRRLLACRELGIEPLVQP
                     YPNDAHTDLWTRFEQSYFAVLELNKGRLSEVQHAFAAAEVLTMVQRLPEARELVREIE
                     AQMTGG"
     gene            complement(1787..>2240)
                     /locus_tag="PLQ54_14140"
     CDS             complement(1787..>2240)
                     /locus_tag="PLQ54_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94456.1"
                     /translation="MARPGGAGGGRAASGRGDWRLEAEVQRARAAAEEGARQQSLLDD
                     LARMALAVIRKQLPGVDTPRRLAREVAEDAAKILPRGADDEDQATAVAEILAPLVRER
                     ARIEVLDREIAAQRARNELAEVVAVAVTARTGDPRLGLLAYHLTAMYG"
BASE COUNT          363 a          804 c          749 g          324 t
ORIGIN      
        1 cgtggacgcg gagccgttgt tcccggtggg gggccgcgct gagcatgggg agctggagca
       61 cgcgctcggc caggcggcac accgggccgg acttgccctg ctgacggagc cgcagcagga
      121 cggggccgag taccgaggcc tcgtcctcgt accgctcccg gcgcacctgg tgaatgcgca
      181 gctcgtcgga gtcgtgcggc tccggtatcg gcctcacacc cggcggacac gagcatggta
      241 cgcgagacta cacgagcagg cagccaagtg gctgaacacc ctcaggaagc tggcgggaga
      301 agacccgccc gaccccgacc atgcaacatc aaggtcgact caaggtacct agatcaaggg
      361 tagctcaaag tacgctcaag ggcttgttca atgcctgatc aaaggagaag taagcaacct
      421 ctactagcac gccgccacat agtggcggcc ccccccaaac gaaggactgg gagggtcttc
      481 tcgaacacgt cctcaccaac aagcgtcgat cccgacgccg cccgcgctcg ccagtaccga
      541 gccccttctg ccagcaagag gtggtccacc ccgagggagt ggctgctgca cgacgcccgg
      601 gcgccgagcg ccccaggcgg ggtggtggcg cgcgttggtc gccgaggggg aggtggccgt
      661 cggcggccgt accaaagaac tcaaacgctc caggccgtct ccgaagccga atcgcaaacc
      721 cagtcgccaa tcaaggaggc catccatgca gtgcaagacc gccatcaacc aagaactccc
      781 ggagcgccag gtccgcagcc gtgggcgccg ccgccgggcg cccacgccgc gacctcccgc
      841 cttggagatc cccctggatc gggtcttcct gtaccacgag gcgctcagcg cgctcccgac
      901 gggcaacgtc gacgagttcg cgctgctgaa gaggtccatc gagagcatcg gtcagttggt
      961 ccccatcttg gtctgggacg gcgtgctgat cgatggccga cgtcgcctgc tcgcgtgccg
     1021 agagcttggg atcgagccgc tggtgcagcc ctaccccaat gatgcccaca ctgacctgtg
     1081 gacgcgcttc gagcagtcct acttcgccgt gctggaactg aacaaggggc gcctgagtga
     1141 agtccagcac gccttcgcgg ccgccgaagt gctcaccatg gtccagcggc tacccgaggc
     1201 acgtgaactc gtacgggaga tcgaggccca aatgactggc gggtgaccgg cagcgacctc
     1261 gatccgtcgt gtggttctgc ccgcccattt gatcctggag tcgatcagca tgatcgggac
     1321 tgcgcacagc acgtcgtcgg cgcatggcgc cggtgcagtt ggcgcttgtg gccgcggggt
     1381 tggcgccccg cctcgagaac accgcgaaga gggccggcag gagggtcggc gcctcggtgg
     1441 aagagctgct ggcagggggc gtccaatagc cttggggcca caaggcccca aggctatgcc
     1501 caaagttcgc gcccccgggt cccagggcag agccgacaag aacttggggc tgtcggcagg
     1561 gctgtcgcga acgagggccc gcctgtccag cgcgagccac ccgaggtcgc tgatgcggct
     1621 cgctcggacc tcgctcggac cctcggcgag gggcggacgc tcgccatgct ccccgctagc
     1681 gcccccacgg agacggcgga gggagctgct cgctccgccc tggcacccgc gtgtgacctc
     1741 accggtcggc gttgcggtgt tccgacggcc gtgccggccc cgccgctcac ccgtacatcg
     1801 cggtcaggtg gtaggcgagg agccccagcc gaggatcgcc ggtgcgggcg gtcaccgcaa
     1861 ccgcgaccac ctcggcgagt tcgttccgag cgcgctgggc tgcgatctcg cggtccagaa
     1921 cctcgatcct cgcccgctcc cggaccagcg gtgccaagat ctcggccacc gcggttgcct
     1981 gatcctcgtc gtccgcgccc cgcgggagga tctttgccgc gtcctcggcc acctcgcggg
     2041 cgagccggcg tggggtatcg acacccggca gctgcttgcg gatgacggcc agcgccatgc
     2101 gggcgaggtc gtccaacagg ctctgctgcc tcgcaccctc ctccgccgcg gccctcgccc
     2161 gctgcacctc cgcctccagc cgccagtctc cgcggccgct cgccgctcgg ccgccacccg
     2221 ctcctcctgg gcgcgccatc
//
LOCUS       DAOSPL010000826         2238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48482_length_2238_cov_3.894182, whole genome shotgun sequence.
ACCESSION   DAOSPL010000826 DAOSPL010000000
VERSION     DAOSPL010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48482_length_2238_cov_3.894182"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..471)
                     /gene="rpsL"
                     /locus_tag="PLQ54_14145"
     CDS             complement(55..471)
                     /gene="rpsL"
                     /locus_tag="PLQ54_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOS94457.1"
                     /translation="MPTISQLVRHGRKPKIRKPKAPALKKLFNAQKREVRDIAGAPQK
                     RGVCLKVWAIQPKKPNSALRKVARVRLSNRQELTAYIPGIGHNLQEHSVVLVRGGRVR
                     DLNSVKYHVVRGILDAAGTENRRQGRSRYGTKRPKE"
     gene            1111..2166
                     /locus_tag="PLQ54_14150"
     CDS             1111..2166
                     /locus_tag="PLQ54_14150"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOS94458.1"
                     /translation="MHRIAVIPADGIGPEVTREAMKVLAVAAQKTGFRYEAVEFDFGG
                     ERYLRTGEVLPDSALAELAQFDAIYLGAVGHPDVAPGVLEHGLLLKLRFELDQYINLR
                     PVKLYPGVYTPIKDKGPEDIDFVVVRENTECLYCGIGGQLHKGTPHEVATQVMLYTRH
                     GVERCIRYAYELARTRPEKKLTLVDKANVLTYGHELWGRVFAEVGQEYPDVQQDHAYV
                     DACCMWFVKNPEWFDTIVTCNMFGDIITDLGAMIQGGMGIAASGNINPQGVSMFEPIH
                     GSAPKYTGQNVINPLAAICAAQMLLEYLGEKEAARMVDQAISDTLAAGRIPDLSVRSG
                     LKTDQIGDMIAQAVADG"
BASE COUNT          381 a          723 c          729 g          405 t
ORIGIN      
        1 tctccccgag gcgatgcttg cctgctctcg cgtcagcgag cccgtccggc ccggttattc
       61 cttgggccgc ttcgtgccat atcgcgaacg gccctggcgg cgattctcgg tgccggcggc
      121 atccaggatg ccgcggacga cgtggtactt caccgagttc agatcccgca cgcggccgcc
      181 gcgcaccaag accaccgagt gttcctggag gttgtgaccg atgccgggga tgtatgccgt
      241 cagctcctgc cggttgctga gccggacacg agcgaccttc cgcagggccg agttcggctt
      301 cttgggctgg atcgcccaga ccttgaggca cacgccgcgc ttctggggtg cgcccgcgat
      361 gtcgcgcacc tcacgcttct gggcgttgaa cagcttcttc agcgcgggag ccttgggctt
      421 gcggatcttt ggtttgcgcc cgtgacgcac aagctggctg atcgtcggca tgcgccggcc
      481 tccttactcg gcttgcggat cagacggtta ccgccgtctc tgcaggcccg ggagacactc
      541 atcccccgcc cagcagcacg gtacggctga cacgaaagga cgctaacggg actcccctgc
      601 cggagatccg agtgtcccgc ggtcgctgac agtcgtccgg agtccggtcg gcgctcgact
      661 cgacagaggc ccccgtgcgg cgaacgtccg cactcactcg ttcgagccca gcgatcttcc
      721 cggttcaggg cgtgcgtcaa ggagcgccca agtcaggaac aaacaagaca ggtactcatc
      781 acactgcgcg ttcgggccgc accctcagga cctcgccggc tccgtggacg gagccccggg
      841 cccgctcagt tgcgacggcc cgcagccgca gcgccacggg acggtagcgg accggttggt
      901 gtccggtcat acccgttccg gtgcacccgg gccgccaatc acaggggcgg ccccgtgcgc
      961 acgaagggac agtctatcag cgggccgcgt gcaagtcaag cggattcggc gctgtttttg
     1021 tgccggcccg atcacgcgtg gacacccgtg ctataatggc ggcccacctg caaggaacca
     1081 ctgccccgca cccgagagga gcaccattcc gtgcacagga ttgccgtgat ccccgctgat
     1141 ggtatcggcc ccgaggtaac tcgcgaggcc atgaaagttc ttgccgtggc tgctcagaag
     1201 accggcttta ggtacgaggc tgtcgagttt gacttcggag gcgagcgcta ccttcggact
     1261 ggggaggttc ttccggattc cgcactggcc gaactggcgc agttcgacgc catctacctg
     1321 ggcgccgtgg gacatcccga tgtggcgccc ggagtgctcg agcacggcct tctgctgaag
     1381 ttgcggttcg agctcgatca gtacatcaac ctccgcccgg tcaagctcta ccccggtgtc
     1441 tacacgccga tcaaggataa gggcccggag gatatcgact ttgtcgtcgt gcgcgagaac
     1501 accgagtgcc tctattgcgg gatcggtggg cagctgcaca agggcacgcc gcacgaggtg
     1561 gcgacccagg tgatgctcta cacgcggcac ggcgtcgagc gctgcatccg ctatgcctac
     1621 gagctggctc gcacacgtcc ggagaagaag ctgaccctgg tggataaggc gaatgtgctc
     1681 acctatggtc acgagctgtg gggccgcgtg ttcgccgagg tgggccagga gtaccccgac
     1741 gtgcagcagg accacgctta tgtggatgct tgttgtatgt ggtttgtcaa gaacccggaa
     1801 tggttcgata ctatcgtcac ctgtaacatg ttcggcgaca tcatcacgga tctgggtgcc
     1861 atgatccagg ggggcatggg tatcgcggct tcgggcaaca tcaatccgca gggtgtgtcg
     1921 atgttcgagc cgatccacgg ctcggccccc aagtacaccg gccagaacgt gatcaacccg
     1981 ctggcggcca tatgtgcggc ccagatgctg ctcgagtacc tgggcgagaa ggaggcggct
     2041 cggatggtcg accaggccat cagcgacacg ctggcggccg gcaggatccc cgacttgtct
     2101 gtccgcagtg ggctcaagac ggatcagatc ggcgacatga tcgcccaggc ggtggccgac
     2161 ggttagggcg gcactccggg aaccggctcg cgctgccaaa gcgtcacctt ctgagagacc
     2221 agccggtcga ctccattg
//
LOCUS       DAOSPL010000827         2238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48492_length_2238_cov_2.619789, whole genome shotgun sequence.
ACCESSION   DAOSPL010000827 DAOSPL010000000
VERSION     DAOSPL010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48492_length_2238_cov_2.619789"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PLQ54_14155"
     CDS             complement(<1..412)
                     /locus_tag="PLQ54_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94459.1"
                     /translation="MKHRMPYPAWVALTMLTCVGLNAGAWGQVTNFTSRTRQIKVANL
                     LVSSARYDNARPPGPTNLENPDPHVWYILDQSPLKPAHWDFVNPLAPPVVTQDIIDRW
                     QVRGDPWVGSLQLGQPITKNMGPYWEVVLNEANLP"
     gene            complement(522..>2238)
                     /locus_tag="PLQ54_14160"
     CDS             complement(522..>2238)
                     /locus_tag="PLQ54_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94460.1"
                     /translation="GAGANASTWYGPLYMPPGAEIIFLDADANTPASLGCSTDPSAGP
                     LGNDPAVPDIMVRLHPDPTGRTQFFRIPWNSATGRFDLLTPDQTAPLDADGDGTMDTT
                     AYIDYPRDGVIYVAGNARVSGRLATPGNPDLRPDWEYNITLIAEGNVYVEGNLLRPSD
                     RDPALGEYDTARGLALNSRIALLAGESVVVNGTRILMRLVQTNAPIVPNQRGVLGRSH
                     WELRAGDQLTGAFSFTAVDDWNGDDRADSTAENADIFLKLRVKGTPGAAFSLFVNNTP
                     YDFDRNPAATGPQPFRYLSGAVQIIRIPLRVGGTDYVPFDDAVGQSNIFRIAAATTLR
                     EPLELYSFCIEPRVGDAVPIDAIPDDPAVPESLELQVSGLVHGGEVYPMDAIVSALMY
                     AQNGTYFVIPGDHFDPSAAAIDRNGDGVIAEYVYPPSGAQQDVNGDGVMDPSDVDLDF
                     SGQEDWIESRRYNMRLTVNGAIAPSTMPDILSVAAWCDKWSWWDTEHRVKEQSGPWAA
                     PGTPNWGMIRYVYDPGLRSGRRLFYTYTAPDGRVRTRDYLPRFPRLPVSDDLIYTGSV
                     VPPAG"
BASE COUNT          361 a          702 c          791 g          384 t
ORIGIN      
        1 cgggcaggtt ggcctcgttg agcacgactt cccaataggg ccccatgttc ttggtgatgg
       61 gctgtccgag ctggagggag ccgacccacg ggtcgccgcg gacctgccag cggtcaatga
      121 tgtcctgtgt gaccaccgga ggcgccagcg ggttgacgaa gtcccagtgg gctggcttga
      181 ggggcgactg gtccaggatg taccagacgt gcgggtccgg gttctcgagg ttcgtcggcc
      241 cagggggccg cgcgttgtcg tagcgcgccg acgagacgag gagattcgcg accttgatct
      301 gccgggtgcg cgacgtgaag ttggtcacct ggccccaggc gccggcgttg agccccacgc
      361 aggtcagcat ggtcagagcc acccacgccg gatagggcat cctgtgcttc ataggaggtc
      421 acacccccgc gatccacagc cgatccgctg agtccgcccc ggcggagtcg acataccgct
      481 tggcccgtcc attgccggaa gcatggccca ccgggcctct gctatcccgc gggcggaacc
      541 acactgcctg tgtagatcag gtcgtcgctg accggaaggc gcgggaaccg cgggaggtag
      601 tcccgggttc gcacacggcc atccggagcg gtgtaggtgt agaacagtct ccgacccgag
      661 cgcaggccgg gatcgtagac gtaccggatc attccccagt tgggcgtgcc gggagccgcc
      721 cacgggccgc tctgttcctt cacgcgatgc tccgtatccc accaggacca cttgtcgcac
      781 caggcggcca cggagaggat gtcgggcatg gtgctgggcg caatcgcacc attgacggtc
      841 aaccgcatgt tgtagcggcg cgactcgatc cagtcttcct gaccactgaa gtcgagatcg
      901 acatccgacg gatccatgac cccgtcgccg ttgacatcct gctgggcccc ggagggtgga
      961 tagacgtact cggcgatgac gccgtcgccg ttgcggtcga tggccgcggc cgatgggtcg
     1021 aagtggtcgc ccggtatcac gaagtaggtg ccgttctggg cgtacatcag ggcgctcacg
     1081 atggcgtcca tggggtagac ctcgcccccg tggaccaggc cactcacctg cagctccagg
     1141 ctctcgggca cggctgggtc gtccggtatg gcgtcgatgg gcacggcgtc cccgacccgg
     1201 ggctcgatac agaaggagta cagctccagg ggctcgcgga gcgtggtcgc ggcagcgata
     1261 cggaagatgt tggactgccc cacggcgtcg tcgaagggca cgtagtcggt tcccccgacc
     1321 cgcaggggaa tccggatgat ctgcaccgcg ccgctcaggt accggaaggg ctgcgggccc
     1381 gtggcggcgg gattccggtc gaagtcatag ggcgtgttgt tcacgaacag gctgaaagcg
     1441 gctcccggcg tccccttcac acggagcttg aggaagatgt cggcgttctc ggccgtggag
     1501 tcagctcgat catccccgtt ccagtcgtcc accgcggtga aggagaacgc tcccgtgagc
     1561 tgatcgccgg cccgcagctc ccagtgcgag cgtcccagca cgccccgctg gtttgggacg
     1621 atgggggcgt tggtctgcac caagcgcatt agaatgcgtg ttccgttcac gaccacgctc
     1681 tcgcccgcga ggagggcaat gcggctgttg agcgcgagcc cgcgcgcggt gtcatactcg
     1741 ccgagtgccg gatcgcgatc ggacggccgg aggaggttgc cctcgacgta gacgttccct
     1801 tcggcgatca gcgtgatgtt gtactcccag tcgggccgca gatcgggatt gccgggtgtg
     1861 gcgagccgtc cgctgacccg cgcgttgccg gccacgtaga tgacgccatc gcgcgggtag
     1921 tcaatgtagg ccgtggtgtc catggtaccg tcgccatcgg catcgagcgg cgccgtctgg
     1981 tcgggcgtca gcaggtcgaa cctgccggtg gccgagttcc acgggatgcg gaagaactga
     2041 gtgcggcctg tgggatcggg atgcaggcgg accatgatgt ccggcactgc cggatcattg
     2101 ccgagcggac cggccgacgg gtctgtggag caccccagag aagcgggggt gtttgcatcg
     2161 gcatccaaga agatgatctc ggcgcccggc ggcatgtaca gaggtccgta ccaggtgctg
     2221 gcattggcac cggccccg
//
LOCUS       DAOSPL010000828         2237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48502_length_2237_cov_3.656279, whole genome shotgun sequence.
ACCESSION   DAOSPL010000828 DAOSPL010000000
VERSION     DAOSPL010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48502_length_2237_cov_3.656279"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PLQ54_14165"
     CDS             <1..749
                     /locus_tag="PLQ54_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94461.1"
                     /translation="DGQPMGCLLNFACHVNVVGGSQVSADYPGQFAAAMKARQGAGHV
                     GLFANGCCGNLCQIDVYDPDLEDWGHEWAHYMGERLADDVLEIEAGMAYTGELKLDSR
                     LVELQLPMRRIPDSLLDWAREVQQKPELQNIVDSTYAGMAFELREEVRSSPTFPGIVQ
                     ALRIGDVGVVMLPGEVFVEFGLDIKLNSPAQRTFVVELSNGVVGYVPTREAFAGKGYE
                     QRTATSSRLSPVAGELMVDAALSMLDSMFL"
     gene            911..2143
                     /locus_tag="PLQ54_14170"
     CDS             911..2143
                     /locus_tag="PLQ54_14170"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOS94462.1"
                     /translation="MRTCLLGTACVVLLSISASVVTAQEPEWIREDEAAALARAKAED
                     KLVLVDIWANWCGWCKKLDQDVFTKPEFAEAAKDFVLLKVDSDANEGYLEKTGQEGLP
                     TTAFLLPDGRLLCARSGYMPVEAYVAMLDEAKSLRGKLDADVGAMTLDDALATAQLWL
                     QWGNGGQAGRIFEALEARQGLTDEQLRSVDLSQALGAIGSNDMEKATALLGRRIAPGL
                     RVTEFDPSDSATLKRALRGVAMLWLDVITGGQIVSFSMQDQTGLCIVPGEMEGQKAFL
                     AAVTTLQALPDPAGCTAEEAARIGHLLCQFSLTDLAMPYLERGGPASAADLVAAQVAA
                     GKPATALETANAALEGEVTGQAKGLLLAAKTLALAAATRTDEAKATRDELLALEGLDP
                     DVLELWTSFFDDETMAQL"
BASE COUNT          363 a          706 c          796 g          372 t
ORIGIN      
        1 cagacggtca gcccatgggg tgcctgctga acttcgcgtg ccacgtgaac gtggtcgggg
       61 ggagccaggt ctcggccgac tacccgggcc agttcgcggc ggccatgaag gcgcggcagg
      121 gggcgggcca tgtggggctc ttcgccaacg gatgctgcgg caacctgtgc cagatcgatg
      181 tctacgaccc cgacctcgag gactggggcc atgagtgggc ccactacatg ggtgagcgcc
      241 tggccgatga cgtgctggag atcgaggccg ggatggccta caccggtgag ctcaagctcg
      301 attcgcgcct cgtcgagctg caactcccga tgcggcgcat ccccgactcg ttgctcgact
      361 gggcgcgcga ggtccagcag aaaccggagc ttcagaacat cgtcgatagc acctacgcgg
      421 gcatggcgtt cgagctgcgc gaggaggtgc gttcctcgcc cacattcccg gggatcgtcc
      481 aggcattgcg gatcggcgat gtgggcgtgg tcatgctgcc gggcgaggtg ttcgtcgagt
      541 ttgggctcga catcaagctc aactcgcccg cgcaacggac cttcgtggtg gagctctcaa
      601 acggcgtcgt gggctacgtt ccgacgcggg aagccttcgc gggcaaaggg tacgagcagc
      661 gaacggcgac gtcgagccgc ctgtctcccg tggcggggga gctcatggtg gatgccgccc
      721 tatcgatgct cgactccatg ttcctgtagt ggcgggcacc atcgagcttc actccccggc
      781 ccgccccagg gaccagatct gtgggaactc cagttcggct ttcgctccgt tgctgcccgc
      841 cgtcggctat actggaacca tctggacccc gcaccgcttt gagtccacct accccctggg
      901 aggggatcac ttgcgtacat gtctgctcgg gacagcctgc gtagtattgc tctccatctc
      961 ggcatccgtg gtcacggcgc aggagccaga atggattcga gaggacgagg cagcggctct
     1021 cgccagagcc aaggccgagg acaaactcgt gttggtcgac atctgggcca actggtgtgg
     1081 ctggtgcaag aaactcgacc aggacgtctt taccaagcct gagttcgccg aggccgccaa
     1141 ggacttcgtt ctgctcaagg tcgatagcga cgccaatgag gggtatctcg agaagacggg
     1201 acaggagggc ctccccacca ccgcgtttct gctgcccgac gggaggcttc tgtgtgcccg
     1261 gtccggctac atgcccgtcg aggcctatgt cgccatgctg gacgaggcca agagcttgcg
     1321 cggcaagctg gacgccgatg tgggcgcgat gacgctcgac gatgcgctgg ctacggcgca
     1381 gctgtggctc cagtggggca acggggggca ggccggccgg atctttgagg ccctggaggc
     1441 ccgccaaggg ctcaccgacg agcagctccg gtccgtggac ctgagccagg cgctcggtgc
     1501 catcggcagc aacgacatgg agaaggccac cgcgctcctc gggaggcgca tcgcgccggg
     1561 cctgagggtg acggagttcg accccagcga ctcggccacc ctgaagcgcg ctctgcgggg
     1621 cgttgccatg ctctggctgg acgtcatcac cggggggcag atcgtatcct tcagcatgca
     1681 ggaccagacg ggcctgtgca tcgttcccgg cgagatggag gggcagaagg cgtttctcgc
     1741 ggcggtgacg acgctgcagg ccctccctga tccggcgggg tgtacggccg aagaggcggc
     1801 gaggatcggt cacttgctgt gccagttctc gctgaccgac ctggcgatgc cgtaccttga
     1861 gagaggcggg cccgcatcgg ccgccgacct ggtggccgcg caggtggcgg ccggcaagcc
     1921 ggccacggcc ctggagaccg cgaacgcggc gctcgagggg gaggtcactg gccaggccaa
     1981 ggggcttctg ctggcggcga agacgctggc actggcggca gccacacgga ccgacgaggc
     2041 caaggcgacc cgtgacgaac tcctggcgct cgaggggctg gaccccgacg tgctcgagtt
     2101 gtggacgagc ttcttcgacg acgagacgat ggcgcagctc taactcggcg ccgtcaccgg
     2161 gtttgcgcag tggctctggg gccgcggggc atttcgtccg cggccccctg cttctgccgg
     2221 agcaggaggg ccatgcg
//
LOCUS       DAOSPL010000829         2237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48508_length_2237_cov_3.246563, whole genome shotgun sequence.
ACCESSION   DAOSPL010000829 DAOSPL010000000
VERSION     DAOSPL010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48508_length_2237_cov_3.246563"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLQ54_14175"
     CDS             <1..302
                     /locus_tag="PLQ54_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012930537.1"
                     /note="responsible for the addition of glutamate residues
                     to the C-terminus of ribosomal protein S6; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HOS94463.1"
                     /translation="GAALWLSGCAFVHKASEAEDAQAKAFTTRPGKGALYIYRSELLG
                     AALGLEVCGVDVLLSDSGPVVTEVNACPGLKAFEEATGVDLADRIIVAAEEVAKG"
     gene            389..>2237
                     /locus_tag="PLQ54_14180"
     CDS             389..>2237
                     /locus_tag="PLQ54_14180"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS94464.1"
                     /translation="MRRRTTRASLGAWLAVAGWVAGGAWAQQADQDLAAGLQSMAAKS
                     YAEAAKQFQAAAQADPTAAPAHFFLGQALYRQAGEATNTADKAQLLDQAAAALSKALE
                     LAPAIPQTRLYLGRVRVEQGRYAEGLAEFNAEMAISRPQRREEIYNGIGMAYAGMGRL
                     VDAETAFRTCIDLDPNYCEARYNLADVYLRMRQAEKAIDELDKLNKTMREYRSYLSRL
                     NKQIERGTRKPTLTQEWVDQKYRYAEEFATRQPQAFKLAGRVYHSVREYGNARNAYRQ
                     ALKEANDGNESDVDARTSVAVESLADAEDRVLRRNLVLDPWKILTVADEDLEEILKLN
                     SEFAPAHNARGECYLLQAQLFLPNEDRGIKPKTLTDARQSLDRAVEIYAKYANDPAAL
                     APLRSPENWARAEYNLGRVDRAENKLQSAVAHFEKARQLRPGYPEATALLAETQAELG
                     NAEVARALVDEALQAEPDAEVLYNVQGQLLMALNRPTEAARAFRAAIDREPGMAEAHI
                     NLGMALYQTESWSAARKSFEQALTILPKSQVLTLASRRAELYWLVGRTYASDGLAQEA
                     IDAYNEALALNPSYLAAERDLGRAYQTVGNLDAAESAFRSALALAEASDN"
BASE COUNT          427 a          725 c          752 g          333 t
ORIGIN      
        1 cgggcgcggc gctgtggctg agcggttgcg ccttcgtgca caaggcttcg gaggccgagg
       61 acgcccaggc caaggccttc accacgcgcc cgggcaaggg tgcgctctac atctaccgca
      121 gcgaactgct gggtgcggcg ctggggctcg aggtgtgtgg agtcgacgtg cttttgtccg
      181 actcgggacc ggtggtcacg gaggtgaacg cgtgtcccgg gttgaaagca ttcgaggaag
      241 caactggagt ggatctggcg gatcgaataa tcgtggcggc tgaggaagtc gccaagggat
      301 aacctcgcca agcgggcctt aggcgatcgg gcagtggtgt gccgatggcc gtatggcggt
      361 ttgtggcggg ggactgcgag gtatcgccat gcgcagacgg accacacgag cgagcctggg
      421 ggcctggctg gcagttgccg gctgggtcgc cggaggggcc tgggcccaac aagcggatca
      481 ggacttggcg gcaggactgc agtcgatggc ggccaagtcc tatgccgagg cggccaagca
      541 gttccaggcg gccgctcagg ccgaccccac cgccgcccct gcccacttct tcctgggcca
      601 agcgctctac cgccaggccg gagaggccac gaacacggcc gacaaggcgc aactgctgga
      661 tcaggcggcc gcggcgctca gcaaggccct cgagctggcc cccgccatcc cccaaacacg
      721 gttgtacctg gggcgggtac gagtcgagca aggccgctac gccgagggac tcgcggagtt
      781 caacgccgag atggccatct cgcgtcccca gcggcgtgag gagatctaca acggcatcgg
      841 tatggcctat gccggtatgg gccggctcgt cgacgccgag acggcattcc gcacgtgcat
      901 tgatctggat cccaactact gcgaagcacg ttacaacctg gccgacgtct acctccgcat
      961 gcgccaggcc gagaaggcta tcgatgaact cgacaagctg aacaaaacca tgcgcgagta
     1021 ccggagctac ctctcacgcc tgaacaaaca gatcgaacga ggcacccgga agccgacact
     1081 gacccaagag tgggtggatc agaagtaccg ctatgccgag gagttcgcca cacggcaacc
     1141 gcaagcattc aagctcgccg gccgtgtcta tcattccgtg cgggagtatg gtaacgcccg
     1201 caatgcctac cggcaggctc tgaaggaagc caacgacggc aatgagtccg acgtcgatgc
     1261 ccgcaccagc gtggcggttg agagcctggc ggatgccgaa gaccgcgtcc tccgccggaa
     1321 cttggtgctc gatccctgga agattctgac cgtagccgat gaggatctcg aggagatcct
     1381 caagctcaac agcgagtttg cgccggccca taacgctcga ggcgaatgct acctgctgca
     1441 ggcccagctc tttctgccca acgaggaccg cggtatcaag cccaagaccc tgacggacgc
     1501 gcggcagtcg ctcgatcggg ccgtggagat ctatgccaag tatgccaacg acccggcggc
     1561 tctggcgccg ctgcgcagcc cggagaactg ggcccgggcc gagtacaacc tcggacgggt
     1621 cgaccgggcc gaaaacaaac tgcagtcggc cgtggcgcac ttcgaaaagg cccggcagct
     1681 ccgcccgggc tatccggaag ccaccgccct tctcgcggag acgcaggcgg agttggggaa
     1741 tgccgaggtg gcccgggcac tcgtggatga ggccctgcaa gctgagccgg acgccgaagt
     1801 gctctacaac gtccaggggc agctgctgat ggccctgaac cggcccaccg aggcagcccg
     1861 ggcgttccgg gcggccatcg accgcgagcc gggcatggcg gaggcccata tcaacctggg
     1921 catggcccta taccagactg agtcatggtc agcggcgcgg aagagcttcg agcaggctct
     1981 caccatccta cccaagtcgc aggtgctcac cctggcttcg cgccgggccg agctgtactg
     2041 gctcgtgggg cggacctacg cctcggacgg tctggcccaa gaggccatcg atgcctacaa
     2101 cgaagcgctg gcgctgaacc cctcctacct cgccgccgag cgcgacctgg gtcgagccta
     2161 ccagactgtc ggcaatctgg acgccgccga gagcgccttc cggtcggctc tggccctggc
     2221 cgaggccagt gacaatg
//
LOCUS       DAOSPL010000830         2235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48560_length_2235_cov_3.566514, whole genome shotgun sequence.
ACCESSION   DAOSPL010000830 DAOSPL010000000
VERSION     DAOSPL010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48560_length_2235_cov_3.566514"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="PLQ54_14185"
     CDS             <1..1185
                     /locus_tag="PLQ54_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94465.1"
                     /translation="APWNQLGPFWVRVPFCIVGIAYFLPLSVARSLWVFYFVFMFQTF
                     IGAQAGYTMPMVQAYPVRAFVGQQMWGGILVSGLYLVWTARHHLKRTLRAALSGAGAP
                     DGEGELLSPRGALVGILLGVVVICAWGHAAGAGLVATAVVFGLYFLTHLVAVRLVCEG
                     GMLYVQHPYRPLNFLLSCVGTARLQRTQIPMLALFDHVWMLDNRSPLMPPLMLTQRIA
                     DAAGLTRRRMAPALASALVVAAVCSVYAYLRVVYEYGGRQLDPWFTEYYSYTLYSRWS
                     QDLVTVGAPPDPTGVWVMGAGAATFMGVLKLYHAYPKWALHPIGFLMGASWPMINFWF
                     PVMLAWLIKGAVLRVGGVTLYRRLAPGFLGLILSEFVCAAIWTLVGVLTRSETAYQVF
                     AI"
     gene            1281..>2235
                     /locus_tag="PLQ54_14190"
     CDS             1281..>2235
                     /locus_tag="PLQ54_14190"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HOS94466.1"
                     /translation="MPGQSPCLLVFDVYNLVFRAFYALPPMTTATGTPTNAVLGVANM
                     VLKLLEELQPDAVAAALDPPTPSFRHQEYAEYKATRQPTPEDLIQQFPLVREFFDAMN
                     IPQIEVDGYEADDVLGALAARAPAAGMRAVLVTGDKDAYQLVNDQVSICISDRSMREN
                     TLLDAEGVREKLGVRPDQVADLKALSGDPSDNIPGVRGIGPKRALKLLSWAEHIEDML
                     ARPEAIEDEKLRAMVVENAEELLRWKRLCVLKCDMPLPGGLVELSRREPDLDRLGELF
                     SRLQLYRLAQRLGVGARGEKAQAAAEAAAPAVRGAAGSGVDA"
BASE COUNT          312 a          719 c          791 g          413 t
ORIGIN      
        1 gcaccatgga accagctggg gcccttctgg gtgcgcgtac ctttctgcat tgttggtatc
       61 gcctacttcc tgccgctctc ggtggcgcgg agcctgtggg tgttctactt cgtgttcatg
      121 tttcagacgt tcatcggggc gcaggcgggg tacaccatgc cgatggtcca ggcctatccg
      181 gtgcgcgcgt tcgtgggaca gcagatgtgg gggggcatcc ttgtctcggg gctctacctc
      241 gtgtggacgg cgcggcacca tctgaagcgc accctccgag ccgcgctctc cggcgccggg
      301 gctcctgatg gcgagggcga gctcctctca ccccgcgggg cgctcgtcgg catccttctg
      361 ggcgtggtcg tcatctgcgc gtgggggcat gccgccggag ccgggctggt ggccacggca
      421 gtcgtgttcg ggctctactt cctgacccac ctggtggcag ttcgcctggt gtgcgagggc
      481 ggcatgttgt acgtccagca tccgtaccgc ccgctgaact tcctgctctc atgcgtgggt
      541 accgcacgcc ttcagcgcac tcagataccc atgctggcac tgttcgacca cgtgtggatg
      601 ctcgacaacc gcagcccgct gatgccgccc ctgatgctga cccagcgcat tgcggacgcg
      661 gcgggactga cgcgcaggcg gatggcgccg gcgttggcct cggcgctggt cgtggccgcc
      721 gtgtgctccg tctacgcgta tctgcgcgtg gtgtacgagt atggcgggcg gcagctcgac
      781 ccgtggttca ccgagtacta ctcatacacg ctctacagcc ggtggtcgca ggatctggtc
      841 accgtcggcg cgccgccgga cccgacgggt gtctgggtca tgggcgccgg tgcggcgacg
      901 ttcatgggcg tgctgaagct ctaccacgcc taccccaaat gggcgctgca cccgatcggg
      961 ttcctgatgg gcgcttcgtg gcccatgatc aacttctggt tcccggtgat gctggcctgg
     1021 ctcatcaagg gggccgtgct ccgcgtgggc ggggtgaccc tctaccgccg gctggcgccg
     1081 ggctttctcg ggctgatcct gtcggagttt gtgtgtgcgg ccatctggac gctggttggt
     1141 gtgttgacgc gctcggagac ggcctatcag gtgttcgcga tataggcaat agggccgtaa
     1201 ggagacggac gcggggggaa gcaaaggccc cgccgcccct ggcgccgaat cggctggcac
     1261 tcgcctccca ggaggatctc atgccgggtc agtccccgtg tctgctcgtg tttgacgtgt
     1321 acaacctggt cttccgggcg ttctacgccc tgccgccgat gacgacggcc acggggacgc
     1381 cgacgaacgc ggtgcttggt gtcgccaaca tggtgctcaa gctgctggag gaactgcagc
     1441 ccgatgccgt ggcggcggcg ttggaccccc ccacgccttc gttccgccat caggagtacg
     1501 ccgagtacaa ggcgacgcgg cagcccacac cggaggatct gatccagcaa ttcccgctgg
     1561 tgcgcgagtt tttcgacgcc atgaacatcc cccagatcga ggtcgacggc tacgaggccg
     1621 acgacgtcct cggcgccctt gccgcgcggg ctcccgcggc ggggatgagg gccgttctgg
     1681 tcaccggcga caaggatgcc taccagcttg tgaacgacca ggtctccatc tgcatcagcg
     1741 accggtcgat gagggagaac acgcttctcg atgccgaggg cgtccgcgag aagcttggcg
     1801 tgcgtcccga ccaggtggcg gacctcaagg cgctgtcggg cgacccatcg gacaacattc
     1861 cgggcgtgcg cgggatcggg ccaaagcgag cgctcaagct cctgagctgg gccgaacata
     1921 tcgaggacat gctcgcgcgc ccggaagcca tcgaggatga gaagctccgc gccatggtgg
     1981 ttgagaacgc cgaggagctg ctccgctgga agcggttgtg cgtgctgaag tgcgacatgc
     2041 cgctgccggg cggcttggtc gagctgtcgc ggcgcgagcc cgacctcgac cggctgggcg
     2101 agctgttctc gaggctccag ctctaccgcc tggcccaacg cctgggcgtc ggcgccagag
     2161 gcgagaaggc gcaggcggcc gctgaggccg cggctccggc ggtgaggggc gcggccgggt
     2221 ccggcgtgga tgcgg
//
LOCUS       DAOSPL010000831         2228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48760_length_2228_cov_2.859641, whole genome shotgun sequence.
ACCESSION   DAOSPL010000831 DAOSPL010000000
VERSION     DAOSPL010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48760_length_2228_cov_2.859641"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /gene="zwf"
                     /locus_tag="PLQ54_14195"
     CDS             <1..1482
                     /gene="zwf"
                     /locus_tag="PLQ54_14195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOS94467.1"
                     /translation="IFGATGDLTSRKVVPALFELSRAGYLAPETAVVGAARRVKSTEG
                     FRAEMAAAIRAHARTQPIAEADLALFLRRVHYQPLEFEVPSDYTALREFLETPSRGRT
                     FPRNRVAYLATAPAFFSMVTHNLREAGLLYEPGGEPWSRVVFEKPFGHDLQSAQALNR
                     QLSQCLAESQICRIDHYLGKDTVQNLLAFRFGNGLFEPIFNRRYVDHVQVTVAEDQGI
                     EGARGAFYDQTGALRDVVQNHVLQLLCLVAMEPPSVCGAEEIRAEKVKVLQALCAEDA
                     GFGWAVRGQYGEGPLGRAYRDEDRIAANSNTETYAAMRVYIENWRWAGVPFLLRTGKR
                     MPVRRTEIVVVFRRPPTQLFRTVRCVGDVCRLAAMEPNLIIFRIQPDEGVLMDLMVKR
                     PGMNMDLHRVTSRFYYRDAFPQALPDAYERLLVDVIRGDQTLFTHSTEIERAWAFLTP
                     LLDYWGAVPPRDFPNYAAGSWGPSAADRLFDGLGGHWRNTREP"
     gene            1577..>2228
                     /gene="pgl"
                     /locus_tag="PLQ54_14200"
     CDS             1577..>2228
                     /gene="pgl"
                     /locus_tag="PLQ54_14200"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOS94468.1"
                     /translation="MPGGQDPIRLIVAPDCMDRVATDFAAACQAAVAHRQRFAVALAG
                     GRTPRALYGRLSSAPFRDQVPWSHIEVFLGDERAVPPDHLESNWRMVCEALLARVPIP
                     PGNRHRPVAEHPDPRSAADQYSGLLAERLPHTPDGIPVLDLVLLGMGEDGHTASLFPG
                     STALETEDRWYVANWVEALGVWRLTVTFPVLRAARQVWVLVMGEHKAKQLARVLSGG"
BASE COUNT          336 a          745 c          758 g          389 t
ORIGIN      
        1 atcttcgggg cgacggggga tctcacgagc cggaaggtgg tccccgcgct gttcgagttg
       61 tcccgtgccg ggtacctggc gcccgagaca gccgtcgtgg gtgccgcgcg tcgtgtgaag
      121 agcaccgagg gcttccgggc tgagatggcc gccgccatcc gggcccacgc gcggacgcag
      181 ccgatcgccg aagcggacct ggcgctcttc ctgcggcgag ttcactacca gccgctcgag
      241 tttgaggttc catcggacta caccgcgctc agggagttcc tcgagactcc gagccgaggg
      301 cgcaccttcc cgcgcaatcg cgttgcctac ctggccacgg caccggcgtt cttctcgatg
      361 gtcacccaca atctgcgcga ggctgggctg ctctacgagc cgggaggcga gccgtggtcc
      421 cgggtggtct ttgagaagcc cttcgggcac gacctccaga gcgcccaggc tctgaaccgc
      481 caactcagtc agtgcctggc cgagtcgcag atctgccgca tcgatcatta cctcgggaag
      541 gacacggtcc agaacctgct ggcgttccgc ttcggcaatg ggctgttcga gcccatcttc
      601 aaccgccgct acgtcgacca cgtgcaagtc acggttgccg aggatcaggg catcgagggc
      661 gcccggggcg ccttctatga ccagacgggc gcgcttcggg acgtcgtcca gaaccatgtg
      721 ctacagctcc tctgcctggt ggccatggaa cccccttcgg tctgcggtgc cgaggaaatc
      781 cgggccgaga aggtcaaggt cctgcaggcg ctatgcgccg aggacgcggg cttcgggtgg
      841 gcggtccggg gccagtacgg cgagggccct ctgggccgtg cgtaccgcga tgaggaccgc
      901 atcgccgcca actccaacac ggagacctac gcggcgatgc gcgtgtacat cgagaactgg
      961 cggtgggcgg gggtaccatt cctcctgaga acgggcaagc gcatgcccgt acgccgcacc
     1021 gagatcgtcg tcgtcttccg tcgtccaccc acccagctgt tccggacggt tcgctgcgtg
     1081 ggcgacgtgt gccggcttgc cgccatggaa ccgaacttga tcatcttccg catacagccc
     1141 gacgagggtg tgctgatgga cctgatggtg aagcgacctg gcatgaacat ggatctccac
     1201 cgggtcacgt cgcgcttcta ctatcgcgat gctttccccc aggcgctgcc ggatgcttac
     1261 gagcggctgc tggtggatgt gatccgaggg gatcagactc tgttcacgca ctcgaccgag
     1321 atcgagcggg cctgggcctt cctgacaccc ctcttggact actggggcgc cgttccccct
     1381 cgggacttcc ccaactatgc tgccgggagc tggggcccgt cggctgccga ccgtctgttc
     1441 gacgggctcg gcggccactg gaggaacacc cgcgagccct gagtcgacac ctgacactcg
     1501 cggagggtcc acggcggcac gtgtcgaaag acggtctgcg ctccgtgcgc cctatctgac
     1561 gcgaagggag gcccgggtgc cgggaggtca ggatccgatt cgtctcatcg tggctccaga
     1621 ttgcatggac cgggtggcga ccgatttcgc cgcggcatgc caggcggctg tcgcccatcg
     1681 ccagcgcttc gcggttgcgt tagcgggtgg ccgaacgccc cgggctttgt acggtcgcct
     1741 ctcgtccgcc ccctttcgcg atcaggtgcc gtggtcgcac atcgaggtat tcctcggaga
     1801 cgagcgcgcc gttccgcccg accacctgga gagcaactgg cgcatggtct gtgaggcgct
     1861 gctcgcgcgt gtgcccatcc cgcccgggaa ccggcaccgc cccgtggctg agcacccgga
     1921 ccccaggtcg gccgccgacc agtacagcgg gttgcttgcg gagcgcctgc cgcacacgcc
     1981 cgacggcata ccggtgctcg atctggtcct gctgggcatg ggcgaggatg gccacaccgc
     2041 ctcgctgttc ccggggagca ccgcgctcga gaccgaggac cgatggtatg tcgccaactg
     2101 ggtggaggca ctgggagtct ggaggctcac ggtcaccttc ccggtgctcc gagccgcgcg
     2161 ccaggtctgg gttctggtga tgggtgagca taaggccaag cagttggccc gggtactgag
     2221 cggcgggg
//
LOCUS       DAOSPL010000832         2228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48762_length_2228_cov_2.655775, whole genome shotgun sequence.
ACCESSION   DAOSPL010000832 DAOSPL010000000
VERSION     DAOSPL010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48762_length_2228_cov_2.655775"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="PLQ54_14205"
     CDS             complement(<1..1091)
                     /locus_tag="PLQ54_14205"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOS94469.1"
                     /translation="MSAMGGWFVVLVSTVLPMAGSAEEPAWAAALFPNTDPPAQCEPR
                     FRRMAAEEIPVGDRRGGQVTVELLHRDVYGETQTAQARIFVPPALVDDPTLRVPLVHA
                     AGYEIDAGGASGYLVAGFVVTTPHAHPQNPISRGPNLDTAILHGARALPFIDDSRVLI
                     QGGSAGGYTTLMLAAETFPLTAAMPDVPPTNWGYNAAYFIRNRERARQVPAGETQSPL
                     PVLNVVIGLADDGVKMLGPDTGSDAWLHMSPISHLETITCPTQVTFSTADMLVPIDQM
                     SRALVRKPPAGMFPEGFTMRLNDCVAPASARRTLLDVLDRDRYELFVVAAPHGAEELR
                     PGASPTRPAAHVPLPFSEQRTFSLVVIDEG"
     gene            1209..1655
                     /locus_tag="PLQ54_14210"
     CDS             1209..1655
                     /locus_tag="PLQ54_14210"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOS94470.1"
                     /translation="MRQTIESVLAGKSVAVAGVSRRPRGFGRQLYIALRQRGYTTYAI
                     NPHAESIGGDPCYASPRMLPRPVDWLLAVTPRSATAQVVREAAQAGITRVWMQPGSEC
                     PEAIQAAREAGMVTVTRACLFLHLEPVTWPHSWHRALAKVFGRLYR"
     gene            complement(1732..>2228)
                     /locus_tag="PLQ54_14215"
     CDS             complement(1732..>2228)
                     /locus_tag="PLQ54_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00375 family protein"
                     /protein_id="HOS94471.1"
                     /translation="SLALRRQDGRGVVGNYGMDPRLGKYHRTFCEECGQVVTAEPPAR
                     ACTRCGGTKVTFGVLDRIVEIADRPSPVHPPHRPPYHHQVPLQFVPKLGAVAMTRLLN
                     RFGSEMAVLHHAAPDELASVVGTRLAEHIVAARDGSLLLQPGGGGRFGRVVASPRGAQ
                     LRLL"
BASE COUNT          357 a          769 c          786 g          316 t
ORIGIN      
        1 ccctcatcga tgacgaccag gctgaatgtc ctctgctcgc tgaagggcaa gggcacatgg
       61 gccgcgggac gggtcgggct ggcgcccggt ctcagttcct cggcgccgtg gggcgcggcc
      121 accacgaaca gctcgtagcg atcacggtcg agcacgtcca gcagcgtcct acgggcgctg
      181 gctggcgcaa cgcagtcgtt gagccgcatg gtgaagccct cggggaacat cccggcgggt
      241 ggcttccgca ccaaggcgcg cgacatctgg tcgatgggga cgagcatgtc ggccgtcgag
      301 aatgtgacct gggtgggaca cgtgatcgtc tcgaggtgcg atatcggtga catgtggagc
      361 caggcatcgg agcccgtgtc gggtccgagc atcttgaccc catcgtcggc tagcccgata
      421 acgacgttga ggaccggcag cggcgactgt gtctcgccag caggcacctg cctggcccgc
      481 tcgcggttcc gaatgaagta ggccgcgttg tagccccagt tcgtgggcgg aacgtcgggc
      541 atggctgcgg tcagcgggaa cgtctccgcc gccagcatga gtgtcgtata cccgcccgcg
      601 ctgccgccct ggatgagcac tctcgagtcg tcgatgaaag gcagtgcccg cgcaccgtgc
      661 aggatcgccg tgtcgagatt ggggccgcgc gagatcgggt tctgcggatg ggcgtgaggc
      721 gtggtcacca cgaagcctgc cacgaggtag cccgacgctc cgcccgcgtc gatctcgtag
      781 cccgcagcgt gaacaagcgg cacccggagg gtggggtcat ccaccagggc gggcgggacg
      841 aagatccgtg cctgggccgt ctgcgtctcg ccatatacat cgcggtggag cagttcgacc
      901 gtcacctggc cacccctgcg gtcgccgacc gggatctcct cggccgccat gcgccggaag
      961 cgcggctcgc attgcgccgg cgggtcggtg ttcgggaaca gcgccgccgc ccatgccggc
     1021 tcctctgctg accccgccat cgggagcacg gtcgagacga gcaccacgaa ccatccaccc
     1081 atcgctgaca tggccaccac cctctcggct gtcacggctc acgttcccga cccggcggca
     1141 gccgacctcc gcgggaggcc gcgagggcga gcaagcagaa ggtcttcgac cgaacggggg
     1201 gacgcggcat gaggcaaacc atcgagtcgg tcctcgcagg caagtcggtg gccgtagcgg
     1261 gtgtgtcgcg caggcccaga ggcttcggtc ggcagctcta catcgcactg agacaacgcg
     1321 gctacacgac gtacgccatc aacccgcatg ccgaaagcat tggcggcgac ccgtgctacg
     1381 ccagcccccg gatgctcccg cggcccgtcg actggctact cgccgtcaca ccgcggagcg
     1441 cgacggcgca ggtcgtccgc gaggccgcac aggcgggcat cacgcgcgtc tggatgcagc
     1501 ccggctccga gtgcccggaa gccatccagg ccgcgcggga ggcaggcatg gtcaccgtca
     1561 cgcgcgcgtg cctgttccta cacctcgagc ccgtgacatg gccccacagc tggcaccgag
     1621 cgctggcgaa ggtgttcggc aggctctacc gctgagggca tacgggggtg ccgttgcggg
     1681 cgatcccgcc gaggcgccca gcaccgacga tcggcgggtc gcccggccga cctacagcag
     1741 ccgcagctgc gcgccccggg gactcgcgac cactcgaccg aaccggccgc cgccgccggg
     1801 ctggagcaag agagatccat cacgcgcagc cacgatgtgc tcggccaggc gcgtgcccac
     1861 cacggacgca agctcgtcgg gggcggcatg atgaagcacc gccatctcgc tgccaaaccg
     1921 gttcagcagc cgcgtcatcg ccacggcgcc gagcttcggg acgaactgca gggggacctg
     1981 gtggtggtag ggcggccggt gcggcgggtg gacggggctt ggccggtcag cgatctcgac
     2041 gatgcggtcc agcacaccga aggtgacctt cgtgccgccg caccgggtgc acgcgcgcgc
     2101 cggtggctcg gcggtgacca cctggccgca ctcctcgcag aaggttcggt ggtacttgcc
     2161 gagccggggg tccatgccgt agttgcccac gactccgcgc ccgtcctgac gacgcagagc
     2221 gagactga
//
LOCUS       DAOSPL010000833         2226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48823_length_2226_cov_3.072778, whole genome shotgun sequence.
ACCESSION   DAOSPL010000833 DAOSPL010000000
VERSION     DAOSPL010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48823_length_2226_cov_3.072778"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PLQ54_14220"
     CDS             complement(<1..764)
                     /locus_tag="PLQ54_14220"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOS94472.1"
                     /translation="MAHIAPEDQMVIESPEQVEVVMELAGLGSRFAAALLDTMCLAAL
                     MVLAGLLLWGMAALVAHSGGSGALASLGGLGLALAIALGFGVFLAYYIVCEMRLNGQT
                     PGKRVVGIRVVRDNGQPVRLLDSALRNLIRFFEATAGAYSLTFICLLVTARRKRLGDL
                     AAGTIVVRATSPQAPALLTTDLSAIGLPPEWCTAIEENVGEVSQQEYEFIGNLVERAS
                     RLPYQVVTRMAVDTAWPLMQRMGILVTPEQMVSPTL"
     gene            complement(773..1429)
                     /locus_tag="PLQ54_14225"
     CDS             complement(773..1429)
                     /locus_tag="PLQ54_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HOS94473.1"
                     /translation="MAIHDDFGDIAESIRARMDVIDTTREFAYTESRQIVRDSAAAIR
                     ATHRGDGAMAAEALARTAEAVRVLLGRLAGEPILYGAGFVQDAIKEYVEAAVTSCLEA
                     EAPLPSPVELRVPEAAYLNGVAEAVMEIRRHIVDEIRLERLSEAERKLERAEDIYHVL
                     MSFDYPDAVALGLRRRLDTVRSVLERTRSDVVTAIRQASLERKMASLESRLSPSSLGD
                     "
     gene            complement(1439..1846)
                     /gene="acpS"
                     /locus_tag="PLQ54_14230"
     CDS             complement(1439..1846)
                     /gene="acpS"
                     /locus_tag="PLQ54_14230"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOS94474.1"
                     /translation="MIRGVGVDIIEVGRVEEALTRRPRLLRRLFTPAEVAYCRSKPAS
                     QYSHFAGRFAAKEAVAKALGRSCAWHDVVVSHDPLGRPVVELSGRAREAVGDGTITLT
                     ISHSRDYAVAYAVFDVSPPADRSRPERPDEEPA"
     gene            complement(1946..>2226)
                     /locus_tag="PLQ54_14235"
     CDS             complement(1946..>2226)
                     /locus_tag="PLQ54_14235"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOS94475.1"
                     /translation="LVLDELRRRGVTSILIWGSRSGDEAGTVASTPQSGLVDAIISLR
                     FVESQGVVRSGLAILKSRGTAHSTDIRPYQITSQGLSVVSRRVTVYDD"
BASE COUNT          409 a          809 c          673 g          335 t
ORIGIN      
        1 tacagggtcg gcgagaccat ctgctcgggc gtcaccaaga tccccattcg ctgcatcagt
       61 ggccacgcgg tgtccacggc catgcgggtg accacctggt acgggagccg gctcgcgcgc
      121 tccaccagat tgccgatgaa ctcgtactcc tgctggctga cttcccccac attctcttcg
      181 atggccgtgc accactcggg cggcagcccg atcgccgaca ggtctgtggt gagcagggcc
      241 ggcgcctgag gtgacgtcgc tcggaccacg atcgtgcccg cagcgaggtc gcccaggcgt
      301 tttcgacggg ccgtcacgag taagcagatg aaggtcagcg aataggcccc agccgtcgcc
      361 tcgaaaaaac ggatgaggtt ccgcaacgcc gaatccagga ggcggaccgg ctggccatta
      421 tcccgaacca cccggatccc aaccacgcgc ttgcccggtg tctggccgtt gagccgcatc
      481 tcgcacacga tgtagtacgc cagaaacacc ccgaacccca gcgcgatcgc caacgccagc
      541 ccgagcccgc ccaacgacgc cagcgcgccg cttccgcccg aatgcgccac cagcgcggcc
      601 atgccccata gaagaagacc agccagcacc atcagggctg cgaggcacat ggtatcgagc
      661 agagccgccg caaaccgcga ccccaggccg gccagctcca tgaccacctc gacctgctcg
      721 gggctctcga tcaccatctg gtcttccgga gcgatgtggg ccatggcctg tcctagtcgc
      781 cgagagacga gggcgagagc cgcgactcca gggaggccat cttccgttcg agcgacgcct
      841 gacgaatggc agtcaccaca tcggaacggg tgcgctccag taccgaccgt accgtgtcga
      901 gccgccgccg aagccccagg gccaccgcat cggggtagtc gaagctcatc aggacatggt
      961 agatgtcctc ggcccgctcg agcttgcgtt cggcctccga cagtcgttcc agacggatct
     1021 catcaacaat gtgccggcgg atctccatga ccgcctcggc cacaccattg aggtaggcag
     1081 cctccggcac ccgcagctcg acgggtgagg gaagcggcgc ctcggcctcc agacacgaag
     1141 tgactgctgc ctcgacatac tccttgatcg cgtcctgcac gaagcccgcc ccgtacagga
     1201 tgggctcgcc ggcaagccgc ccgaggagaa cgcgcaccgc ctcggccgtg cgcgccaaag
     1261 cctccgcggc catggcaccg tcgccgcggt gagtcgcccg tatcgccgca gccgagtcgc
     1321 gcacgatctg tcgcgactcg gtgtatgcaa actcccgagt tgtgtcgatc acatccattc
     1381 gcgcgcggat cgactcagcg atatccccaa aatcgtcgtg gatcgccatg cacctccatc
     1441 aggcgggctc ctcatcaggg cgctccggcc gagaccggtc ggcggggggg ctcacatcga
     1501 acaccgcata ggcgacggca tagtcccgtg agtgtgatat ggtcagcgtg atggtgccat
     1561 cgcccacggc ctcccgggcc cggccggaca actcaaccac cggcctcccg agcgggtcgt
     1621 gagacacgac cacatcatgc cacgcacacg atctccccag agccttggcg accgcttcct
     1681 tcgccgcgaa acgaccggcg aagtgtgagt actgggatgc cggcttcgag cggcagtacg
     1741 ccacttccgc cggggtgaac agccgccgca ggagtctggg ccgccgtgtc agcgcttcct
     1801 cgacccgccc gacctcgatg atatccactc cgacgcccct gatcatgaac cgcgggccgc
     1861 gtggccccac acctccacac tgggtgtctc gatcgcatag gagactcgaa cgggccgcca
     1921 ggcggtaggg cgtccgcttc ccgggctagt cgtcatacac cgtaacgcgc ctcgagacga
     1981 cactcaggcc ctgcgacgtg atctggtacg ggcgaatatc ggtgctgtgt gccgtgcccc
     2041 ggctcttgag aatcgccagg cccgagcgca ccactccctg cgactccaca aaccgcagac
     2101 tgatgatggc gtccaccagg cccgactgag gggtgctggc cacggtgccc gcctcgtctc
     2161 cgctccggct gccccagatg aggatcgagg tgaccccacg acgccgcaac tcgtcgagca
     2221 cgaggc
//
LOCUS       DAOSPL010000834         2224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48899_length_2224_cov_2.673582, whole genome shotgun sequence.
ACCESSION   DAOSPL010000834 DAOSPL010000000
VERSION     DAOSPL010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48899_length_2224_cov_2.673582"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="PLQ54_14240"
     CDS             complement(<1..1180)
                     /locus_tag="PLQ54_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94476.1"
                     /translation="MSSTRRLVGPAGLALALGLSAACAPAALAARYTVWRLAEGRAPT
                     VSADGRLVWFWSRTGAQWQLHRSETDGANMVALTGPGTGWPDVPGQTSANVRAVSVCA
                     SGSTLVAVARYVLHPGSSPVDLSGSLTEPNGELLDALVTRDGSRVVALAKGIGAPLGP
                     DARLMVGSPAGAPWVTVWEGPLGAADAKEPRLAASADGTVLALVIGDSSDGGEAPGVG
                     RVSVWALQEDGTWASDATDPQPARGLPCLSADGQRLAYVDGLDGTRVLTFDRARRAAS
                     SIATGATGPITSLAMDDFGRRIAACAAGRVYYAAFTGHTSHEVPGLSGARSVSLSADG
                     DVLAAETSRGIMVGLARGGGAVPAVPSARPVPAGPSAAATASGRQIRTFDETLADLYR
                     L"
     gene            complement(1173..>2224)
                     /locus_tag="PLQ54_14245"
     CDS             complement(1173..>2224)
                     /locus_tag="PLQ54_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94477.1"
                     /translation="SPDGRNVVCSAVRTVDGEQRAGLWVINIQSRAPTLLLPATLKSA
                     PFAPCYSPNGELVAYVRSEDDRATRAGLWVMTVLRKQTQLFALHETLSLSTSSGLMWM
                     PDNVTLRFVASASGGTQEITSLWELNTRTGKMKHVLTFPLDERISDAACSPDGTRVAY
                     IRTRGDKRELVVRDPQTPEGRVVAQPVAPPVLIPRPFESDLSFTGDSGAIVYVSGTEL
                     RMVDVVEGGHLASPAYATECAENLTEILHALERYSRGHEGNVPAPGADATHPEPFFWV
                     PLLRPLMDRPERLRCPSDAKGEGPTSYELAPGVAGRAWAELRAERQIVLRDREPFHGG
                     KRMVIYSDGRTELVE"
BASE COUNT          348 a          778 c          760 g          338 t
ORIGIN      
        1 ccagacggta gaggtcagcc agagtctcat cgaaggtccg gatctgacgg ccgctcgccg
       61 ttgccgcagc ggacggccct gccgggaccg ggcgcgccga tggaacggcc ggtaccgccc
      121 cgccgccacg ggccagtccg accataatac cgcggctcgt ctctgcggcc aggacatcgc
      181 cgtcggcgct caggctcact gaccgcgccc cggagaggcc gggtacctcg tggctcgtgt
      241 gtcccgtgaa tgccgcgtag tacacccgcc cggccgcgca ggccgcgatc cgtcggccga
      301 agtcatccat ggcgagtgac gtgatcggcc ctgtggcacc cgtcgcgatg ctggacgcgg
      361 cgcggcgggc gcggtcaaag gtcagaacgc gcgtgccgtc gaggccatca acataggcga
      421 ggcgctggcc gtcggcactc aggcacggca gtccgcgggc cggctgggga tccgtggcat
      481 ccgacgccca ggtgccatcc tcttgcagtg cccagactga aacacgcccc acgcccggcg
      541 cctcgccgcc gtctgacgag tcgccgatca cgagcgccag tactgtgccg tcggccgagg
      601 ctgcgagccg tggctctttg gcgtcagcgg cccccagggg cccttcccac accgttaccc
      661 acggtgcccc agcgggcgaa cccaccatca gccgggcatc gggcccgagg ggcgcgccga
      721 tccccttcgc gagggcgacg acccgcgaac catcccgcgt gaccagtgcg tcgaggagtt
      781 caccgtttgg ttccgtgagg ctcccgctca aatcgaccgg agatgacccg gggtgcagca
      841 cgtagcgcgc cacggccacc agcgtagacc ctgaagcgca gacggagacg gcgcggacgt
      901 tcgcgctggt ctgtcccggc acatcgggcc agccggtgcc tggccctgtc agggccacca
      961 tgttcgcgcc gtcggtctca gatcggtgga gctgccactg cgccccggtt cggctccaga
     1021 accagaccag gcgcccgtcc gccgaaacgg taggtgcccg gccttcggcc aggcgccaga
     1081 cggtgtagcg agcggccaac gcggcagggg cacacgccgc ggagagaccg agggccaggg
     1141 cgagtccggc tggcccgacg aggcggcggg tactactcaa cgagctcggt tctaccatcg
     1201 ctgtagatga ccatccgctt cccgccatgg aacggctcgc ggtcccgcag aacgatctgc
     1261 cgctcggctc tgagttcggc ccatgcccgc ccggcaactc ccggggccag ctcatagctg
     1321 gtcggcccct ctcctttggc gtccgacggg caccgcaacc gctcaggccg gtccatcagg
     1381 gggcggagga gcggcaccca gaagaacggc tctgggtgcg tagcgtccgc ccctggggct
     1441 gggacattgc cctcgtgtcc gcgcgaatag cgttcgaggg cgtggaggat ctcggtcagg
     1501 ttctcggcac actcggtggc ataggcgggc gaggccagat gccctccttc gacgacatcg
     1561 accatccgaa gctcggtccc cgagacgtac acaatcgccc cggagtcacc cgtgaagctc
     1621 aggtcgctct cgaacggccg cgggatcagg accggcggcg caactggttg ggcgacgact
     1681 cggccctcgg gagtctgcgg atcgcgcacc accagctccc gcttgtcccc ccgggtccgg
     1741 atgtaggcca ctcgcgtccc gtcgggcgag catgcggcgt cggagattcg ctcatcgagg
     1801 gggaaggtga gtacgtgttt catcttgccg gttcgtgtgt tcagctccca caggctggtg
     1861 atctcctggg tcccaccgct cgcggatgcg acgaatcgca gtgtcacgtt gtcgggcatc
     1921 cacatgaggc ccgaggacgt ggaaaggctg agggtctcgt gcagcgcgaa aagctgggtc
     1981 tgcttccgga gtaccgtcat gacccagagc cccgcacgag tggctcgatc gtcttcactg
     2041 cggacatatg ccacgagctc gccgtttggc gagtagcacg gcgcgaacgg cgccgacttg
     2101 agcgtggccg gcagcagcaa tgtgggcgcc ctggactgga tgttgatcac ccacagcccg
     2161 gccctttgtt cgccatcgac ggtacgtacg gcgctgcaga cgacgttgcg tccgtcgggc
     2221 gaga
//
LOCUS       DAOSPL010000835         2223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_48914_length_2223_cov_4.319188, whole genome shotgun sequence.
ACCESSION   DAOSPL010000835 DAOSPL010000000
VERSION     DAOSPL010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48914_length_2223_cov_4.319188"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="PLQ54_14250"
     CDS             complement(<1..1205)
                     /locus_tag="PLQ54_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94478.1"
                     /translation="MQTRAESARAEPVAETRDEAGRRSPWRFAAVVLFAVVLMPLNSY
                     WVVQSELVRYQGHPTTISLFYNVVFILLVLTLLNLPLRRWAPRLALTPGELMTIYIVL
                     CIGSAGAAHDAIQVLTPVMTHVFYYASPENGWADLIQPHMPTWLCMTDRQSLINMHWG
                     ESNFLAPENYRPWLLPGLWWTAFTVALWSTCLGLTSIFRRRWIHSERLTYPIVMLPLE
                     MVAYRSRALPEGFYKNRMMWLAFGLALGADILNGIHTLKPLWPMIPISPRSAPKLNII
                     SQVLDRPWNAIGYAPVSFYPFVIGLGLLLPTELATSCWVFFWFWKMQLVVGSLFGQNV
                     AQGFPWIEEQEFGAYLAVTAFFVWVSRNYLADVCRQIIHPRTEAGTGSLPEGMSYRTA
                     ALLVVGGFV"
     gene            complement(1347..1865)
                     /locus_tag="PLQ54_14255"
     CDS             complement(1347..1865)
                     /locus_tag="PLQ54_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94479.1"
                     /translation="MEGDASQGRVTARVGGQPITMPYVSDEEKGITADALGLKPPPQA
                     RRQKGEVVRSTINTGESGTPAPLVLQAATYISAKPEDEVLAFYEKEYGAALKDLSGAS
                     QANLGVTVEQASNLQAVRFLLVNGAICQISLDDDPGSAGTRVILSKWAEAPQDLVDGL
                     KALLTSDKPGQG"
BASE COUNT          363 a          750 c          768 g          342 t
ORIGIN      
        1 aagacgaaac cacccaccac cagcagcgcc gccgtgcgat aactcatccc ctcgggcagc
       61 gacccggtac cggcctcggt tctcgggtgg atgatctgcc ggcacacgtc cgcgaggtag
      121 ttccggctca cccagacaaa gaacgccgtc accgccaggt acgccccgaa ctcctgctcc
      181 tcgatccacg ggaagccctg ggccacgttc tggccgaaga gggagccgac caccagctgc
      241 atcttccaga accagaagaa cacccagcac gatgtggcca gctcggtggg cagcaggagg
      301 cccagcccga tgacgaaggg gtagaacgac accggggcat agccgatggc attccacggc
      361 cggtccagaa cctggctgat gatgttcagc ttcggcgccg accgcgggct gatcggtatc
      421 atgggccaca ggggcttcag ggtgtggatg ccgttgagga tgtcggcgcc cagggccagg
      481 ccaaaggcga gccacatcat cctgttcttg tagaagccct cgggcagggc gcgcgaccgg
      541 taggccacca tctccagcgg cagcatcacg atggggtacg tgaggcgctc cgagtggatc
      601 caccgtcgcc ggaagatgct cgtcagtccc aggcatgtcg accacagggc cacggtgaat
      661 gcggtccacc acaggcccgg gagcagccac gggcggtagt tctccggtgc gaggaagttc
      721 gattcgcccc agtgcatgtt gatcagcgac tgccgatcgg tcatgcagag ccacgtcggc
      781 atgtggggtt ggatcaggtc cgcccaacca ttctcgggtg atgcgtagta gaacacgtgg
      841 gtcatgaccg gggtgaggac ctgaatggcg tcgtgggccg ccccggcgga cccgatgcac
      901 agcacgatgt agatggtcat gagctcgccg ggggtcaggg cgaggcgcgg agcccaccgg
      961 cgcaacggca ggtttagcag cgtcagcacc agcaggatga acacgacgtt gtagaacagc
     1021 gagatggtgg tggggtgccc ctggtagcgg accagctcgg actggacgac ccagtagctg
     1081 ttcaggggca tgaggacgac cgcgaatagc actaccgccg cgaaccgcca ggggctccgg
     1141 cggcccgcct cgtcccgcgt ctcggcgacg ggctcggctc gggctgactc ggctctagtc
     1201 tgcatgggct cgctgtcggc acacaagaac gcagcccggc gatccggatg ccgggcgggt
     1261 ttgatccttc gtcagccgtc cgcgcgggtc ctgcggggat ggtcgggacg cgccggcggc
     1321 agccggtctc tcgcgcgaca tggggtctag ccctgccccg gcttgtccga ggtgagaagc
     1381 gccttgaggc cgtcgaccag atcctggggc gcctcggccc atttcgagag gatcacccgc
     1441 gtccccgcgc tcccgggatc atcgtccaga ctgatctggc agatggcgcc gttgaccagc
     1501 agaaagcgca ctgcctgaag gttcgaggcc tgctcgacgg tcaccccgag gttggcctga
     1561 ctggcgcccg acaggtcctt cagcgcggcg ccgtactcct tctcatagaa ggccagcacc
     1621 tcgtcctccg gcttcgcgct gatgtaggtg gccgcctgta gcaccagggg ggcgggcgtg
     1681 ccggactcgc ccgtgttgat ggtgctgcgc acgacctcgc ccttctgccg ccgggcctgg
     1741 ggcgggggct tcagccccaa ggcgtccgcg gtaatgccct tctcctcgtc ggacacatac
     1801 ggcatggtga tgggctggcc gcccactcgc gccgtgacac ggccctggct cgcgtcgcct
     1861 tccacggcga cttccgggcc gggaggcgct ggcctcaccg gctgtggacg cgcgccctgc
     1921 cggcatgcga ggggtcccag gcacagcatt gcgcacgcga tcagggcata gcagtatcgg
     1981 cacatggcca ctccctggtg agaggtcaca gtacgcctca cgcccgccgc cgggcgcgag
     2041 tccatcgcgc acggccggcc aatgggccgg gatcggaggc ccccggccgg cgccgtcgag
     2101 cgtatcctac aacgtgggcg tgtccggttg ctcggggttg ggtcccgccg gggcggggct
     2161 ctccgggccg ccccctccat ccgcaggcgg ctgaggcgca ctgccacgca cgccgccggt
     2221 gcc
//
LOCUS       DAOSPL010000836         2221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_49000_length_2221_cov_2.566020, whole genome shotgun sequence.
ACCESSION   DAOSPL010000836 DAOSPL010000000
VERSION     DAOSPL010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49000_length_2221_cov_2.566020"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PLQ54_14260"
     CDS             <1..682
                     /locus_tag="PLQ54_14260"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94480.1"
                     /translation="EFVANCKASELTTLGIPATVERLRAKWQACIDSESPPLERVYLP
                     AEWAFAADREDRGVVEKWVAEPRYYEAAAGFTIATDPDHYSPPHLPKGIARLHINRGV
                     GWEQQGFPGLDGYGWYFQTITVPEELAAKPHLYLYLRGVNEEAWVYVNGELAFERTLA
                     ATGRSPGDLAGVPFGFDAKRWLKPGDGNQIAIRVMHSTGLGGICAPAMLAGTEEERTT
                     EQLDAYRL"
     gene            complement(797..1972)
                     /locus_tag="PLQ54_14265"
     CDS             complement(797..1972)
                     /locus_tag="PLQ54_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS94481.1"
                     /translation="MAQRPLNVGLIGGGGGAFIVQPHQKAIHFDGTRRVTCAALHPDP
                     AIAMQEAENWAYPIQGYTNYNDLIAQESAKPRGEGIDYALIVTPNHVHFDPAMKCIQA
                     GIPVFCEKPLTVTLDEAAQLVEAVKAKRIPFGVSHTYLGHWTSRLARHIVRSGLIGDV
                     RWVDSYYLQGWLAGRTEDQGSMQAEWRVDPKRAGKSCCGGDIGTHALMQLRFVTGLDV
                     VKLSAQLETFVAGRQLDDHFTVYCQLSNGGRALVRASQIAIGHKNDLGIEVNGTKGTL
                     RWVQEEPESLTIHLKGQPDRVYWRGEVQPNDGFLGDLPADLMAEPTIPSGHPEAFHDA
                     FARLHRCFEADVRAYQAGTYTEADGSKYATVADGHMGILFVEKSVESASKAGAWVDM"
BASE COUNT          356 a          736 c          752 g          377 t
ORIGIN      
        1 cgagttcgtg gcgaactgca aggccagcga actgaccacg ctgggtatac ccgccacggt
       61 ggagcgcctc cgcgccaagt ggcaggcgtg catcgactcc gaaagccctc cactggagcg
      121 ggtctacctg ccggcggagt gggcgttcgc cgcggaccgc gaggatcgtg gggtcgtcga
      181 gaagtgggtc gccgagccgc ggtactacga ggcggcagcc gggttcacca tcgcgaccga
      241 ccccgaccac tactcgccgc cccatctgcc gaagggcatc gcgcggctgc acatcaaccg
      301 cggcgtcggg tgggagcagc agggcttccc tggcctcgac gggtatggct ggtacttcca
      361 gaccatcacg gtccccgagg agctggccgc gaagccacac ctctacctgt acctgcgcgg
      421 cgtgaacgaa gaggcatggg tgtacgtcaa cggggaactc gccttcgagc gcacgctggc
      481 cgcaacgggc aggagcccgg gcgacctcgc cggggtgcca tttggcttcg acgcgaagcg
      541 ttggctgaag ccgggcgatg gcaaccagat cgcgatccgc gtcatgcact caaccggcct
      601 gggcggcatc tgcgcgccgg ccatgctcgc cggcacggag gaagagcgga ccacagagca
      661 gctggacgcc taccggctgt agcggccagg cagtagtcgc acagggcgat ggtgcttcga
      721 gagggccgag ctgaatccga cgggggaagc gcggcgcgct tcccccgctc gctcgttctc
      781 tcgcgatgaa ccggtgctac atgtcgaccc aggcgccggc cttcgatgcg ctctcgacgg
      841 acttctcgac gaacaggatg cccatgtgcc cgtcggccac ggtggcatac ttcgagccgt
      901 cggcctcggt gtaggtgccg gcctgatagg ctcgcacgtc ggcctcgaaa cagcggtgga
      961 gccgggcgaa cgcgtcgtgg aaggcctccg ggtggcccga ggggatcgtg ggctcagcca
     1021 tcaagtcggc aggtaggtcg cccaggaagc cgtcattggg ctggacctcg ccgcgccagt
     1081 agacccgatc gggctgaccc ttgaggtgga tcgtcaggct ctccggctcc tcctggaccc
     1141 atcgcaaggt gcccttggtg ccattcacct cgatgccgag gtcgttcttg tggccgatgg
     1201 cgatctgaga ggcgcggacc agcgcccgtc ccccattcga gagctggcag tagacggtga
     1261 agtggtcatc gagctgccgc ccggcgacga aggtctcaag ctgagccgac aacttcacca
     1321 cgtcgagacc ggtgacgaag cgcagctgca tcagtgcatg agtgccaatg tcccctccgc
     1381 agcagctctt gccggcgcgc ttggggtcga cgcgccactc ggcctgcatg gagccctgat
     1441 cctcggtccg gccggccagc cagccctgca gatagtagga gtccacccag cggacgtcgc
     1501 cgatcagtcc acttctcacg atgtggcgcg ccaggcggct cgtccagtgc ccgaggtacg
     1561 tgtgcgagac gccgaagggg atcctcttcg ccttgacggc ctccaccagc tgggccgctt
     1621 cgtcgagtgt caccgtcaga ggcttctcgc agaacacggg aatgcccgcc tggatgcact
     1681 tcatggctgg atcgaagtga acatggttcg gcgtcacgat cagcgcgtag tcgatgccct
     1741 ctcccctggg cttggctgac tcctgagcga tcaggtcgtt gtagttcgtg tagccctgga
     1801 tcgggtatgc ccagttctcg gcctcctgca tcgcgatggc cgggtccggg tgcagcgcgg
     1861 cgcacgtcac gcggcgcgtc ccgtcaaagt gaatggcttt ctgatgcggc tggacgatga
     1921 acgcgccgcc accaccaccg atgagaccga cattcagcgg ccgttgtgcc atgggatctc
     1981 ccttcctggg atgccgatgt agtggccgcg ggaccgcggt ggacagggca ttgcccaccg
     2041 cgggcttcca cgaccacgtc gggtcttctt ctcctcaagg cccgaggact cctggtgcgc
     2101 gccccgaaag ggcactcaga ctgagcccgt ctcgggaggt gttgccccgt ggggcatgtg
     2161 cactggtggc cgctcatcgc gttcgtgtct gcgagtcccg cgttcgcggc ctacctgacg
     2221 c
//
LOCUS       DAOSPL010000837         2219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_49061_length_2219_cov_3.075786, whole genome shotgun sequence.
ACCESSION   DAOSPL010000837 DAOSPL010000000
VERSION     DAOSPL010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49061_length_2219_cov_3.075786"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PLQ54_14270"
     CDS             <1..688
                     /locus_tag="PLQ54_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOS94482.1"
                     /translation="VTGPTGCGKSTTIAAMLNEINEARYCHIVTIEDPLEFIHRDKKS
                     IISQREVHIDTENFSAALRTVLRQAPDVILIGEMRDVETFEVALAAAETGHLVFSTVH
                     TRSAAETLERILNMFPPHAKDQICMRLSQSLQGVISQKLVPTADMSRRVGAIEIMIVT
                     PTVAKYIEEGRVAEMYSAIDEGGFWGMQTMNQCLCRYFQEGLITEEVALANAGNLTEL
                     RQMVRRHKPE"
     gene            776..1873
                     /locus_tag="PLQ54_14275"
     CDS             776..1873
                     /locus_tag="PLQ54_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOS94483.1"
                     /translation="MAEIDPLLRTLRDRGGSDLHLKVGNPPIIRVDGDLTRLDLPVLR
                     PEDTKRIMYGLLSPERQRVFEETHEMDVSYYLPDVARFRVNVFQQKGYVGGVLRLIPI
                     QVKTIDEMGLPQVLKYLAMRPRGLMLVTGPTGSGKSTTLAAMVDYINENKAAHIITIE
                     DPLEFLHFDKLSCLNQRELGEDTHSFAEALKHCLRQNPDVILVGEMRDLETIQLAITA
                     AETGHLVFATLHTTDAAQTIDRIIDVFPPEQQEQIRAQLAATIEGIVCQQLLQRKGGK
                     GRVPAFEIMLGTPAVKTLIREAKTHQVPTIIQTGLELGMVPLDQYLIGLVKDQVVDYE
                     QALQKCSNPRDFQQRAQRVLGSHLGGATLAT"
     gene            1915..>2219
                     /locus_tag="PLQ54_14280"
     CDS             1915..>2219
                     /locus_tag="PLQ54_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HOS94484.1"
                     /translation="MTDLAYIDELLTDLVKREGSDLLLHEGAPPMMRIHGELVRRTEL
                     PKLENTKDVEDIAFAACDPAQLERFHRDWELDFAYEIPGIARFRVNFMRQRMSTACT"
BASE COUNT          435 a          685 c          717 g          382 t
ORIGIN      
        1 ggtgacgggc ccgacgggct gcgggaagtc gacgaccatc gcggccatgc tcaacgagat
       61 caacgaggcc cgttactgcc acatcgtcac catcgaggac ccgctggagt tcattcaccg
      121 cgacaagaag agcatcatca gccagcgcga ggtgcacatc gacaccgaga acttcagtgc
      181 ggccctgcgc accgtgttgc gccaggcccc ggatgtgatc ctcatcggcg agatgcgcga
      241 cgtggagaca ttcgaggtgg ccctggcggc cgcggagacg ggccacttgg tgttctcgac
      301 ggtacacacg aggtcagcgg ccgagaccct cgaacgcatt ctgaacatgt ttccgcccca
      361 tgcgaaggac cagatctgta tgcgcctgtc gcagtccctg cagggcgtca tctcgcagaa
      421 actggtgccg acggccgaca tgtcccggcg ggtgggcgct atcgagatca tgatcgtgac
      481 gcccacggtg gccaagtaca tcgaagaggg ccgggtggcg gagatgtact cggcgatcga
      541 cgagggcggg ttctggggca tgcagaccat gaaccagtgc ctctgccgct acttccagga
      601 gggcctgatc accgaggaag tggcgttggc caatgccggc aatctcacgg agcttcggca
      661 gatggtgcgt cggcacaagc ccgagtaggg cggcaccccc aacccttggc cggcgccgca
      721 gctggcgccc gggtcccggg tcccgccaca ttcatcgacc agcgaggtgt gacacgtggc
      781 cgagattgat ccgctcttgc gcacgttgcg ggaccggggt ggatccgacc tccacttgaa
      841 ggtgggtaat ccaccgatca tccgcgtgga tggcgacctg acccgcctgg atctgccggt
      901 cctgcggcct gaggacacca agcgcatcat gtatggtctc ttgagcccgg agcgccagcg
      961 agtgttcgag gagacgcatg agatggacgt ctcctactat cttcccgatg tggcgcgctt
     1021 ccgggtcaac gtattccagc aaaagggcta cgtcggaggc gtgttgcggc tgattcccat
     1081 ccaggtgaag accatcgatg agatgggact tccccaggtt ctcaagtacc tcgccatgcg
     1141 cccgcgaggc ctgatgctgg tgacgggtcc caccgggagc ggcaagtcga ccacactggc
     1201 ggccatggtg gactacatca acgagaacaa ggcggcgcac atcatcacca tcgaggaccc
     1261 gctggagttc ctgcactttg acaagttgtc gtgcctgaac cagagggagc ttggtgagga
     1321 tacacacagt tttgccgagg cgctgaagca ctgcctgcgc cagaacccgg atgtcattct
     1381 ggtcggggag atgcgcgatc tggagaccat tcagctagcg atcaccgcgg ccgagaccgg
     1441 gcacctggtg ttcgccacat tgcacacgac cgacgcggcc cagaccatcg accgtatcat
     1501 cgacgtgttc ccaccggaac agcaagagca gatccgcgca cagctggcgg cgacgatcga
     1561 ggggatcgtc tgccagcagc ttctgcagcg caagggcggg aaggggcgcg tgcccgcctt
     1621 cgagatcatg ctgggcactc cagcggtgaa gacactgatc cgtgaggcga agacccacca
     1681 ggttcccact atcatccaga ccggcctcga actgggaatg gtgcctctcg accagtacct
     1741 gatcggcctg gtgaaggatc aggtcgtcga ctacgaacag gccctgcaga agtgcagcaa
     1801 cccacgggat ttccagcagc gtgcgcagcg tgttctgggt tcgcacctgg gcggcgcgac
     1861 tctggcgact tagcccatcg caccgcgcgc gtgtcgccgg cggcctgaga gggtgtgacg
     1921 gacttggctt acatcgatga actcctaacc gatctggtga agagagaggg ctccgacctg
     1981 ctgttgcatg agggcgctcc ccccatgatg cggatccatg gcgagctggt gcgccggacg
     2041 gaactgccca agctcgagaa caccaaggat gtcgaggata tcgccttcgc ggcttgcgac
     2101 ccggcgcagc tcgaacggtt ccacagggac tgggagcttg acttcgcata cgagatccca
     2161 gggatcgcgc ggttccgtgt caacttcatg cgccagcgca tgtcgacggc gtgcacgtt
//
LOCUS       DAOSPL010000838         2218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_49099_length_2218_cov_3.152104, whole genome shotgun sequence.
ACCESSION   DAOSPL010000838 DAOSPL010000000
VERSION     DAOSPL010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49099_length_2218_cov_3.152104"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="PLQ54_14285"
     CDS             complement(<1..1150)
                     /locus_tag="PLQ54_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94485.1"
                     /translation="MVRHTLFLAALAVSGLRAQDTTCQIGPLPARLVEGASFEVTVTY
                     SVAQGRARLNGELKDPHTNVVLVHDEVMVEGRGEHVFTFETPRAVEHRDVSIAAFLGD
                     DWRQPLAPIVSTPVIQVISAAQQEKLEQLAARVDDQLRALGYKPSERGNIAVFAADPA
                     DDVSPALVEALAARGLSVTTLTVEAMSNPFLVTRDRFDLLVLVNPRRFPGPCLETVSA
                     FAHSRGHLMVLGGPAFETLLWPVEGKWVPEEAVQRQLVESLQWPAFADFENGSVAEWT
                     RESNGDQGSSVVVEAGGARGSARCAHIAIRDLKGWDTFATPVLDGPFGPGHDLTCFWA
                     KGGERTSQLSVEWREKDGSRWIAVVPLTTQWKPYAIPASAFAYWHDGAS"
     gene            1401..>2218
                     /locus_tag="PLQ54_14290"
     CDS             1401..>2218
                     /locus_tag="PLQ54_14290"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94486.1"
                     /translation="MTPQAELEALRASIPDLEAAYRRPLERILAEIDFAARMADAQPD
                     NSPKWWPLITQAARLVAEGVRSGRTDLPALVSEAEAILSPLGPAAKEYAVYCCGHAHI
                     DMNWMWTWPETVSVTYDTFSTMDQLMDEFPEFHFTQSQASVYVAMQEYAPELFERIKK
                     RVAEGRWEVAASQWVEGSKNLASGEILCRHLLYTRRWLRDALGLPFDAVKIDWEPDTF
                     GHCWTLPGILTRGGVTRYYHHRASGERLRAMCSGESSQLFWWEGKDGSRVLAFD"
BASE COUNT          394 a          793 c          697 g          334 t
ORIGIN      
        1 ggctggcgcc gtcgtgccag taggcgaagg ccgacgcggg tatggcatag ggcttccatt
       61 gggtcgtgag cggcaccacg gcgatccagc gcgacccatc cttctcgcgc cactcgaccg
      121 acagctggct cgtgcgctct ccgcccttgg cccagaagca tgtcagatcg tgccccggcc
      181 caaacggacc gtcgagcacc ggcgtcgcga aggtgtccca ccccttcagg tccctgatgg
      241 cgatgtgggc gcaccgggcc gaaccccgag ccccacccgc ctcgaccacc acggacgagc
      301 cctggtcgcc attgctctcg cgagtccatt cggccaccga gccgttctcg aaatccgcga
      361 aggcgggcca ctggagcgac tcgaccaact gccgctggac agcctcctcg ggcacccact
      421 tcccctcgac cggccacagc agcgtctcga aagccggccc acccaacacc atcaggtgtc
      481 ccctggaatg ggcgaaagcc gatacggtct cgaggcaggg ccccgggaac cgtcttgggt
      541 tgacgagcac cagcagatcg aagcggtcgc gggtcaccag gaacgggttc gacatggcct
      601 ccacggtcaa cgtcgtcacc gacaggccac gcgcggccag ggcctcgacc agagctggcg
      661 agacgtcatc ggctgggtcc gccgcgaaaa cggcgatgtt ccccctctcg gacggcttgt
      721 agcccagcgc ccggagctgg tcatccactc gcgccgccag ctgttccagc ttctcctgct
      781 gcgcggcaga gatgacctgg atcacgggag tggacacaat gggcgccaag ggctgccgcc
      841 aatcgtcacc gagaaaagcc gcgatactga cgtcacggtg ctcgacggcc ctgggagtct
      901 cgaaggtgaa cacatgctcc ccccggccct cgaccatcac ttcgtcgtgg acgagcacca
      961 cgttggtgtg cgggtccttg agctcgccat tcagccgggc ccggccctgg gccacggagt
     1021 aggtaacggt cacctcgaac gacgcgccct caacgagtcg ggccggcagt gggccgatct
     1081 gacacgtggt atcctgcgca cgaagacccg agactgcgag cgcggcgagg aacagcgtgt
     1141 ggcggaccat ggggttcgac ctccctacgc gtgccagtgg gatcgaacag tcaacgggac
     1201 ggcagcggcc ccgtgccggc gtcagccgcg ccgcccaccg ggccgtccga ggcacgaagc
     1261 ttggcccccg tgcccctctc tcgcttattc gcccgcgacg ccggaaggcc tacgcaaccc
     1321 ggcggacgag gtggctggcg gttcgcgcgg aagaacacaa agacccatcg tctgagcgcc
     1381 acacagaggg agaccccgtt gtgacaccac aagccgaact cgaagccctg cgcgcctcca
     1441 ttccagacct ggaggccgca taccgacgcc ccctggagcg cattcttgcc gaaatcgact
     1501 tcgctgcccg aatggccgac gcccaacccg acaacagccc caagtggtgg ccgctgatca
     1561 cacaagccgc ccggctggtc gctgaaggcg tgcggtcggg ccgcaccgac ctgcccgcac
     1621 tcgtgtccga agccgaggcc attctctccc ccctgggccc cgcggctaag gagtacgcgg
     1681 tgtactgctg cggccacgca cacatcgaca tgaactggat gtggacctgg cccgagaccg
     1741 tatcggtcac ctatgacaca ttctctacca tggaccagct catggacgag ttccccgagt
     1801 tccacttcac ccagagccag gcatcggtct atgtcgccat gcaggagtac gcccccgagc
     1861 tcttcgagcg catcaagaag cgcgtggccg agggccgctg ggaggtggcg gccagccagt
     1921 gggtcgaggg ctcgaagaac ctggcctcag gcgagatcct gtgccgtcat ctgctgtaca
     1981 cgcgccgctg gctgcgagac gccctggggc tacctttcga tgccgtcaag atcgactggg
     2041 agcccgatac cttcggccac tgctggacgc tgcccggcat actcactcgc ggcggcgtga
     2101 cgcgctacta ccatcaccgt gcatcgggcg agcggctccg ggccatgtgc agcggcgagt
     2161 cctcgcagct cttctggtgg gagggcaagg acggatcccg cgtcctggcc ttcgatga
//
LOCUS       DAOSPL010000839         2218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_49102_length_2218_cov_2.668054, whole genome shotgun sequence.
ACCESSION   DAOSPL010000839 DAOSPL010000000
VERSION     DAOSPL010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49102_length_2218_cov_2.668054"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2218
                     /locus_tag="PLQ54_14295"
     CDS             <1..>2218
                     /locus_tag="PLQ54_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94487.1"
                     /translation="VVASVGLADQVAQYVRRRLDRSWAVYLARREIVAERGLSQALND
                     PRYRYDSTGPFKKYNLLYTPSRVDLGAEEVRSVRDVPKWVGGVDSESASAGAALYSLY
                     NVAGPVALPSPKLAEFLKVGENFFSRGGVFYLSLAAGANVDALGFGDGELFRDQWNRR
                     GDRMVLPPGETYGGFCVPKEFSLLYAIITTAVSPESSGRLLAAFGVPETLHATAVADL
                     RRVLAMRLDCADELEWEMRAGEFLATRYGDYFAALDRPTAIVRLTQLARTMDAAGVLG
                     GEGQERRALAFELTHWINKKALGLEEINRVGPFRKVNLIRELVAEARKRNPLVAPDGQ
                     LIGVMTASYKEGERKNGREVPITDVRFSAGARKLEIYAGTAAQHILKDIDPEGREIVT
                     RLFEGFTSPADIRIVGTCTGSDLLTHVPESGLTPIMENVLQRLLDTGLDESLIDANCA
                     VYGGDLARWSGIKDLPENERERLITDIGPRIHLLVVARRGVYRTYEHALQGVDFVDLG
                     IPDPELLDLIDNLPKLVYLMKRGRPNSALVFADGTSGARRRAFSFRYANGKRKVQELF
                     ALDANAVYGAMGLGAGMVDRWRQEMEVSRRQAHELWDHLLAGETEAAVRTYAVMCRRL
                     ARERLADEAVDEEVAARRAGVDATPYRLRSSALGRVLQGLPLQGLDFGTWLVLGGCFV
                     LNGQVSAEELVTARAAFEHAVAALPAHGARPKPLPRATVDAMVAAFVRPRYEPPRVE"
BASE COUNT          348 a          741 c          755 g          374 t
ORIGIN      
        1 cgtggtggcg tccgtggggc tggccgacca agtagcgcag tacgtgcgcc ggcggctcga
       61 ccgctcgtgg gccgtctacc ttgcccggcg cgagatcgtg gccgagcgcg gcttgtcaca
      121 ggcgctcaac gacccgcggt atcggtacga ttcgaccggc ccgttcaaga agtacaacct
      181 gctgtacaca cccagccgcg tcgatctcgg tgccgaggag gtccgctcgg tccgtgatgt
      241 gcccaagtgg gtgggcggcg tcgacagcga atcggccagc gccggggccg cgctctactc
      301 gctctacaac gtggcaggtc cggtggcact gccctcgccc aagctggccg agttcctcaa
      361 agtcggcgag aacttcttct cccggggcgg cgtgttctac ctctccctgg cggccggggc
      421 caacgtggat gccctcggtt tcggcgatgg cgagctgttc cgggaccaat ggaatcgccg
      481 aggcgaccgg atggtactgc cgcccggcga gacctacggc gggttctgtg tccccaagga
      541 gttctcgctt ctttatgcga tcatcaccac ggctgtgagc cccgagtctt cggggcgctt
      601 gctcgcggcc tttggcgtgc ccgagacgct ccacgccacc gccgtggccg atctgcgccg
      661 ggtcctagcc atgcggctcg attgcgccga tgagctcgag tgggagatgc gtgcgggcga
      721 gttccttgcc acgcgctatg gcgactactt cgccgcgctg gaccgtccca ctgccatcgt
      781 ccggctcacc cagctcgcca ggaccatgga cgccgcgggc gtgttgggag gagagggcca
      841 ggagcggcgt gccctggcat tcgaactcac tcactggatc aacaagaagg ccctggggct
      901 cgaagagatc aaccgcgtgg ggccgttccg caaggtcaac ctgatccgcg agctcgtggc
      961 cgaagcccgg aagcgcaacc cattggtggc gcccgatgga cagctcattg gtgtgatgac
     1021 cgcctcctac aaggagggcg aacgcaagaa cgggcgcgag gtgcccatca ctgacgttcg
     1081 cttctcggcc ggcgcgcgga agctcgagat ctatgcaggc acggccgccc agcacatcct
     1141 gaaggacatc gaccccgagg gccgcgagat cgtcactcgc ctgttcgagg gattcaccag
     1201 ccccgccgat atccgcatcg tcggcacgtg caccgggtcg gatctgctga cccacgtgcc
     1261 cgagtcgggc ctgacaccca tcatggagaa cgtgctccag cgattgctcg acacgggtct
     1321 cgatgagtcg ctcatcgacg ccaactgcgc cgtctacggc ggcgacctgg cgcgctggag
     1381 tggcatcaag gatctcccgg agaacgagcg cgaacggcta atcaccgaca ttggcccgcg
     1441 catccatctg ctcgtcgtcg ccaggcgcgg cgtgtaccgg acctatgaac acgcactgca
     1501 aggggtcgac ttcgtcgacc tgggtatccc cgaccccgag ctgctggacc tcatcgacaa
     1561 cctgcccaag ctggtgtacc tgatgaagcg cgggcgccca aacagcgctc tggtgttcgc
     1621 cgatggtacc tccggggctc gacgaagggc gttctcattc cgctacgcca acgggaagcg
     1681 gaaggtgcaa gagctcttcg ctctagacgc caacgccgtc tacggcgcca tgggccttgg
     1741 ggcggggatg gttgaccgct ggcggcagga gatggaggtc tcgcggcggc aggctcacga
     1801 gctgtgggac cacctccttg ccggagaaac cgaggcggcc gtccggacct acgcggtcat
     1861 gtgccgccgg ctcgctcggg agcgactggc ggacgaggcg gtcgacgagg aagtggccgc
     1921 acgccgcgcg ggtgtcgatg cgacgccgta ccgactccgt tcgtctgcgc tgggacgcgt
     1981 gcttcagggg ttgccgctcc agggactgga tttcgggacc tggctggttc tcggcggctg
     2041 cttcgtgctc aacgggcagg tctcggccga ggagctagtc acggctcgcg ccgccttcga
     2101 gcacgccgtg gccgccctgc cagcgcacgg cgcgcggccg aagccgctgc ctcgcgccac
     2161 cgtggatgcc atggtggctg ccttcgtccg gccgcggtat gagcctccca gagtggag
//
LOCUS       DAOSPL010000840         2210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_49313_length_2210_cov_2.774478, whole genome shotgun sequence.
ACCESSION   DAOSPL010000840 DAOSPL010000000
VERSION     DAOSPL010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49313_length_2210_cov_2.774478"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..685)
                     /locus_tag="PLQ54_14300"
     CDS             complement(2..685)
                     /locus_tag="PLQ54_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOS94488.1"
                     /translation="MPGTILVVEDDQDIADSVAYSLRREGYEVEVAYDGSEGVRVGLE
                     QKPDLVILDLMLPGLSGFGVFRELRRRSTVPVIMLTARVSENDRVAGLELGADDYITK
                     PFSLRELVARVKTVLRRAEQAAGPADEVLTVKGLVIDRGRRTVTVDGRQVTLTPQEFA
                     LLECLARHPGRAMSREVLLQQAWSESEYIDPRTVDVHVRLVRGKIEAEPSQPTRILTV
                     RGVGYKLAE"
     gene            complement(794..1681)
                     /locus_tag="PLQ54_14305"
     CDS             complement(794..1681)
                     /locus_tag="PLQ54_14305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOS94489.1"
                     /translation="MSTNLGTIYLADNLEVLTGLASGSANLIYIDPPFNTGKVQQRTQ
                     IRTIRSEDGDRVGFKGRRYETIALGKRGFRDLFDDYLAFLEPRLAEAHRVLAPDGTLY
                     FHVDYREVHYCKVLLDAIFGRECFLNEVIWAYDYGGRSKTRWPPKHDNILVYTKDPRR
                     YTFNWDDIERIPYMAPGLVGPDKAARGKVPTDTWWHTIVPTHGSEKTGYPTQKPLGIL
                     KRIIGASSRKGDVVLDFFAGSGTTGAACLALGRRFVLVDNSPQALEAMARRFAGVAGI
                     QWVGFDPGAHAGDVGPLAP"
     gene            1854..>2210
                     /locus_tag="PLQ54_14310"
     CDS             1854..>2210
                     /locus_tag="PLQ54_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOS94490.1"
                     /translation="MDRLLRKRRTTGGFTLIELLVVIAIIAILAAILFPVFAKAREKA
                     RQTTCLTHAKELGLAMMMYAGDYDDTLPMSYYYINGANSSNGYVQWSGLIMPYTKNMG
                     LFVCPTHEPKGWAPTCF"
BASE COUNT          408 a          747 c          678 g          377 t
ORIGIN      
        1 ctcactccgc cagcttgtag ccgacgcccc gcacggtgag aatccgcgtg ggctggctgg
       61 gctcagcctc gatcttgccc cggaccaagc gcacgtgaac gtcgacggtc cggggatcaa
      121 tgtactcact ctcactccac gcctgctgga ggaggacctc acgcgacatg gctcgccccg
      181 ggtgccgagc caggcactcg agcagggcga actcctgggg cgtcagggtg acctggcggc
      241 catctaccgt caccgtccgc cggccacggt cgatcacgag ccctttcacg gtgagcacct
      301 cgtcggcggg gccggccgct tgctcggcac gccgcagcac ggtcttgacg cgcgccacca
      361 actcgcgaag tgagaatggc ttggtgatgt agtcatcggc tccgagctcg agtccggcca
      421 cgcgatcatt ctcgctgacg cgggcggtca acatgatgac gggcacggtc gaacgtctcc
      481 gaagctcgcg gaacacgcca aagccgctca ggcccggaag catcagatcg aggatcacca
      541 ggtcgggctt ctgctcgagc cccacgcgaa cgccctcgga gccatcatac gcgacctcga
      601 cctcgtagcc ctcacgccgc agcgagtagg cgaccgagtc ggcgatgtcc tgatcgtcct
      661 cgacgaccag aatggtgccc ggcatcgtca tgcctcacct atcatggaat ggggccgcgg
      721 ggcgcgcctc gggacccgga gcgccccctc tcccgcccgc cgaggatgat agcaccctgg
      781 cattatgaca tggttaaggg gccaagggac cgacatcccc ggcgtgggct ccgggatcga
      841 atcccaccca ttgaatgccg gccacgccgg cgaaccggcg ggccattgcc tcgagggcct
      901 gggggctgtt gtctaccagc acaaaccgtc tgcccagtgc gagacaggcg gctcccgtgg
      961 tgccgctgcc ggcgaagaag tccagaacca cgtccccctt ccgcgaggaa gccccaatga
     1021 tgcgcttgag gatgccgaga ggcttctgcg tggggtagcc ggtcttctcc gaaccgtggg
     1081 ttggcacaat cgtatgccac catgtgtccg tgggcacttt gccgcgggct gccttgtctg
     1141 ggccaaccag cccaggggcc atgtagggga ttcgctcgat gtcatcccag ttgaacgtgt
     1201 accgcctcgg gtctttggtg tacaccagga tgttgtcgtg cttgggcggc cacctggtct
     1261 tcgaccggcc cccatagtcg taggcccaga tgacctcgtt gagaaagcac tcgcggccga
     1321 agatggcatc gaggagcacc ttgcagtagt ggacctcacg gtagtccacg tggaagtaga
     1381 gcgtcccatc cggcgccagg acccggtgcg cctcggcaag ccgcggctca aggaaggcga
     1441 ggtagtcgtc gaacagatca cggaagccgc gtttgccgag cgcgatcgtc tcgtagcgac
     1501 gacccttgaa ccccactcgg tcgccatcct ctgagcggat ggtgcggatc tgagtcctct
     1561 gctgaacctt gcctgtgttg aatggcgggt ctatgtagat gaggttggcg gagccggacg
     1621 cgagccccgt gagcacctcc agattgtcgg ctaggtagat cgtaccgagg ttcgtcgaca
     1681 agaccggctc actcctcagc cgcctggcgc ccgcttaacc tcaacctaac actcccctaa
     1741 ccgtgacata acggcgcccc ggtaggctag ttcccgggca gacacccggc cggggacctg
     1801 ccacaaccca gtttactcac ctggctcggc acgcaccgga aaggaaggac acaatggacc
     1861 gactgctccg caagcggcgg accaccggag gcttcacgct catcgagttg ctggtcgtga
     1921 tcgccatcat cgcgatcctg gccgccatcc tattccccgt gttcgccaaa gctcgcgaga
     1981 aagcccgaca gacgacctgc ctcacccatg ccaaggaact cggcctggcc atgatgatgt
     2041 acgccggcga ctacgacgac acgctgccca tgtcgtacta ctacatcaat ggcgcgaaca
     2101 gcagcaacgg ctatgtccag tggagcggcc tgatcatgcc ctacaccaag aacatggggc
     2161 tcttcgtctg cccgacccac gagccgaagg gctgggctcc gacgtgcttc
//
LOCUS       DAOSPL010000841         2209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_49337_length_2209_cov_3.447539, whole genome shotgun sequence.
ACCESSION   DAOSPL010000841 DAOSPL010000000
VERSION     DAOSPL010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49337_length_2209_cov_3.447539"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="PLQ54_14315"
     CDS             <1..1088
                     /locus_tag="PLQ54_14315"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOS94491.1"
                     /translation="WQYRRDPWEGLCVSAMLITLGTSQFARGVHFGAGQIARGTKVDL
                     VTSVIGLGGTLAMLLAGIHSILLTVPLTAAMGVYSLACWPWSASGRPEKALRSEIDKF
                     VTIAALGSIASAGLLQMTQLIAQGISEVAAGEYAPALQLVTPLAIVAGALTLVMFPSM
                     AEAQGAGSMDRLRQITDLATRAFVAVLVPIFGAMAVASRPVMHVVYRGNYPESALIMP
                     AFCVALLLQNVASPAVSSLTSGPHKNMWYSLAMSWAGLAGAAISWWPLVPRFGILGIA
                     IGYAIGSGITACSLFAVAWRINGQAWSSLSVRLALSVIVIVALSWWRQSQPVDFAGDA
                     VAGLAFLLVSSALGWPTVRNMTALRRP"
     gene            1146..>2209
                     /locus_tag="PLQ54_14320"
     CDS             1146..>2209
                     /locus_tag="PLQ54_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOS94492.1"
                     /translation="MSFVLGGSHVHRKSLTGLLRIGLAGWDAASWALSAIALVVARYS
                     MSLSNSDTQIVFAYAALAILLQIAAGTLSKLYRGRHRVASFEEAMLLAITTVGIGITL
                     AVGFALWAPRGYPRVVTFTVPFAALVLMAAGRFLFRTMTRGANAHEAEGVIVYGAGNA
                     GQQLGRLLEYDPDAPFEIVGYIDDDPAKKNLHLSGARVLGGRDKLVDAARAAGVKTVI
                     IAIPTADRTLIRAVSAIADEAGLKTLIMPPTREIVGGRVQLSSLHQIDVTDLLGRAQI
                     KTNLSEISGYLAGKVVLVTGAGGSIGSELARQVNRFGPAELVMLDRDESGLHGTQLSI
                     YNQGLLDTPNMVLCDIRDAEA"
BASE COUNT          340 a          707 c          727 g          435 t
ORIGIN      
        1 ggtggcagta tcggcgcgac ccgtgggagg gcctctgcgt gagcgccatg ctgatcacct
       61 tgggcacctc ccagttcgcg cgcggggtgc acttcggagc tggccagatc gcgcgcggca
      121 ccaaggtcga tctggtgacg agtgtgatcg gactgggcgg caccttggcc atgctcctgg
      181 ctggcatcca cagcattttg ctgacggtgc cgctcacggc ggcgatgggc gtgtactccc
      241 tggcgtgttg gccctggtcc gcgtccggac gtccagagaa ggcgttgcga agcgagatcg
      301 acaagttcgt caccatcgcc gccctgggca gcatcgccag cgccggcctt ctgcagatga
      361 cccagttgat cgcccagggc atctccgagg ttgctgccgg cgagtacgct ccggctctgc
      421 aactcgtcac cccactggcg atcgtggctg gggccctcac gctcgtgatg ttcccgtcga
      481 tggccgaggc gcaaggcgcg gggagcatgg accgactccg gcagattact gacctggcca
      541 cgagggcctt cgttgcggtc ctcgtgccga tcttcggcgc catggcagtt gcctcgcgtc
      601 cggtgatgca tgtcgtctac cggggcaact atcccgaatc agcactgatc atgccggcct
      661 tctgcgttgc gctcctgctg cagaacgtcg cctctccggc cgtttcgtcg ctcaccagcg
      721 gtccccacaa gaacatgtgg tattccctgg ccatgtcgtg ggcggggctc gctggagccg
      781 ccatcagttg gtggcccctc gtgccccggt tcggcatctt gggcattgcg atcggctacg
      841 cgatcgggtc tgggatcacc gcctgttccc tgttcgcggt cgcctggcgg atcaacggtc
      901 aggcttggtc gagcctttcg gtacgcctcg cgctctcagt catcgtcata gtggcgctca
      961 gttggtggcg ccagagtcag ccagttgact tcgctgggga cgccgtcgcg ggcttggcct
     1021 tcctgctggt ctcctccgcg ctgggctggc ccacggtccg gaacatgacg gcgctgaggc
     1081 gtccgtagcc acctggcgcg cacggggcgt ccgcgtgacg ggcgagcaaa cgacggtaga
     1141 ctcaggtgtc ttttgtcctc ggaggttccc acgtgcatcg aaaatcactt accggcctgc
     1201 tccgcatcgg tttggcgggc tgggacgcgg ccagttgggc attgtccgct atcgcattgg
     1261 tggtggctcg ctactcgatg agcctctcga actccgacac tcagatcgta ttcgcgtacg
     1321 cggccctggc catcctgtta cagattgccg caggaaccct ttccaagctg taccggggac
     1381 ggcatcgcgt cgcgtcattc gaggaggcga tgctgctcgc catcaccacg gtgggcatcg
     1441 ggatcaccct ggccgtcggc ttcgccctat gggcaccccg tggctacccg agagtcgtga
     1501 cgttcacggt accgttcgcc gcgctggtct tgatggcggc gggacggttc ttgtttcgca
     1561 ccatgactcg cggggccaac gcccacgagg ccgaaggggt catcgtgtac ggggctggca
     1621 acgcagggca gcagttggga cggctgcttg agtacgaccc ggatgcgccc ttcgaaattg
     1681 tcggttacat cgacgatgac ccggctaaga agaacctaca tttgtcgggt gcccgcgtcc
     1741 tcggcgggcg cgacaaactg gtggacgccg ctcgtgcagc tggggtcaag actgtgatca
     1801 tcgcgatccc aacggccgac cggacgctca tccgagccgt gtcggccatc gccgatgagg
     1861 caggcctgaa gacgctgatc atgcccccca cgagggagat tgtcggggga cgcgtgcaac
     1921 tgtcgagcct gcaccagatc gatgtgaccg acctcctggg acgcgcccag atcaagacca
     1981 atctctcgga aatctccggc tatctggcag ggaaggtcgt cctggtgacg ggcgcgggag
     2041 gctcgatcgg ctcggaactg gcgcgccaag tgaatcgatt cgggccagcc gagctcgtca
     2101 tgctcgatag ggacgagtca gggctgcacg gcacccagct ctccatctat aaccaagggc
     2161 tgcttgacac gcccaatatg gtcctgtgcg atatccggga cgccgaggc
//
LOCUS       DAOSPL010000842         2208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_49371_length_2208_cov_3.244310, whole genome shotgun sequence.
ACCESSION   DAOSPL010000842 DAOSPL010000000
VERSION     DAOSPL010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49371_length_2208_cov_3.244310"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLQ54_14325"
     CDS             <1..290
                     /locus_tag="PLQ54_14325"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOS94493.1"
                     /translation="FGVYLHVGHRTFVELFSGDHAGARDGQSYQHFCLEVDDVAEAVR
                     TLRARGVDVTDPYFGSDRSWQAWLSDPDGNRIELHGYTPESRQLGAGIDVA"
     gene            280..1404
                     /locus_tag="PLQ54_14330"
     CDS             280..1404
                     /locus_tag="PLQ54_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94494.1"
                     /translation="MWPRLCQAVRPGLLTLALAPCLLGRGAVAAHPGAGPWPDGSRWP
                     AHLRGLHFETRHQANEGPGPNDWDRANVSLHPERGARLRIAPGAAGNWTCAELITSET
                     VGYGTYTWSISGDFDQWHRRVVLGMFIYRDDEHEMDVEISKWFSESGLNGQFAVQPVW
                     RDYDGDGEPEPVADPARTGQPGKRHLFSTGAHHELTFRLRWTPRRVEFAGWAGREPAD
                     GDSLWHWTYRPGLAPDADIPNAELGGYHADMNLWLFRGGDGYPLDRLEPTEVWVHDFT
                     YEPLASPPNAPPRIRGIETGPAAVRPGETVRLRCEAQDPDADPLRYFWSAVGGGGELE
                     VAGQRAVWRAPTRSGRYSIACEVRDPVGDHDVGRIAIDVR"
     gene            1450..>2208
                     /locus_tag="PLQ54_14335"
     CDS             1450..>2208
                     /locus_tag="PLQ54_14335"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HOS94495.1"
                     /translation="MSQHENAQGTLPEITVIDYSADGAKEWSASCLAECAPLVSTPGA
                     TWINVDGLDHAPTFETLNTLFGVPQLVIEDIMNADQRPKLEEHDDFVFVVVKMLRFDD
                     DEGCVRGEHVGLILLPHCLISFQEAGGDVFDGVRNRIRANAARFKSRGCGYLLHALLD
                     AVVDEYFVVLERLGDCLEAIEDDVLNDPSPEVLKPLHHLRGEAISLRRSVWPLRETAS
                     RLDRLETPLIDAQCGVYFRDLYDHTVQVVEATEAF"
BASE COUNT          358 a          702 c          770 g          378 t
ORIGIN      
        1 gtttcggggt ctacctgcat gtggggcacc gaacgttcgt cgagctgttc tcgggtgacc
       61 atgccggcgc gcgtgacggc cagtcgtacc agcacttctg cctggaggtc gacgacgtgg
      121 ccgaggccgt tcgcacgctc cgcgcccgtg gcgtcgatgt gacggatccg tactttgggt
      181 ccgaccgcag ttggcaggcc tggctcagcg atccggacgg aaaccggatc gagctgcatg
      241 gctacacgcc tgagagccga cagcttgggg caggcattga tgtggcctag actctgccag
      301 gcggtccggc cgggcctgct gacgctggct ctcgccccat gcctcctggg gcgaggggcc
      361 gtggctgccc atccgggggc ggggccgtgg cccgacggaa gccgctggcc ggcgcacctt
      421 cgcgggcttc acttcgagac tcgtcatcag gccaatgagg ggcccgggcc caatgactgg
      481 gaccgggcga atgtctcact ccatcccgag cggggcgccc ggctgcggat cgcgccgggt
      541 gcggcgggga actggacttg tgctgaactg atcacttcgg agacggttgg ctacggcacc
      601 tacacatggt cgatctccgg ggacttcgat caatggcacc ggcgagttgt gctcgggatg
      661 ttcatctacc gtgatgatga gcacgagatg gatgtggaga tctcgaagtg gtttagcgag
      721 tctggcctga acggtcagtt tgccgtgcaa ccggtgtggc gggactacga cggcgatggc
      781 gagcctgagc cggtggccga ccctgcccga acggggcagc ccgggaagcg ccacctcttc
      841 agcacggggg cgcaccacga actgacgttc cgcctccgat ggaccccgcg gcgagtggag
      901 ttcgccggat gggccggccg ggagccggca gatggtgact ccctgtggca ctggacttac
      961 cggcccggtc tggcgccgga cgccgacata cccaatgccg aattgggtgg gtaccacgcc
     1021 gacatgaacc tgtggctatt ccgagggggt gacggatacc ccctcgaccg cctcgaaccc
     1081 accgaggtat gggtacacga cttcacgtac gagcccctgg ccagcccgcc gaacgcgccg
     1141 ccgcgcatcc gcggcatcga gaccggaccg gctgcggtgc gaccgggtga gacggtgaga
     1201 ctgcgttgcg aggcccaaga tccggatgcc gaccccctgc gctacttctg gtccgctgtc
     1261 gggggcgggg gcgagctgga ggttgcgggc cagcgggccg tctggagggc cccaaccagg
     1321 agcggccggt actccatcgc ctgcgaagtc cgggacccgg tgggcgacca cgacgtgggc
     1381 cggatcgcca tcgacgttcg gtgacttcag ttaggcccgc gccggggcca ggaccggcgg
     1441 gtgagaaccg tgagccagca cgagaacgcg cagggcacac tgcccgagat cacggtcatc
     1501 gactactctg cggacggggc caaggagtgg tcggcaagct gcctggccga gtgcgcaccc
     1561 ctcgtgagca cgccgggcgc cacctggatc aacgtggatg gtctcgatca cgcccccacc
     1621 ttcgagacgc tgaacacgct gttcggtgtt ccacagctag tcatcgaaga catcatgaac
     1681 gccgaccagc gccccaagct cgaagagcac gatgacttcg tgttcgtcgt cgtcaagatg
     1741 ctccgtttcg acgacgacga gggctgcgtc cggggcgaac acgtggggct gatcctgctt
     1801 cctcactgcc tgatctcctt ccaggaggca ggcggtgacg tgtttgacgg cgtccggaat
     1861 cgcatccggg ccaatgcggc tcggttcaag tcgcggggct gtggctatct gctacacgcg
     1921 ctcctggacg ccgtggtgga tgagtacttc gtcgtgctcg aacggttggg agactgcctg
     1981 gaggccatcg aggacgatgt cctcaacgat ccctctcccg aggtcctcaa accgctgcac
     2041 cacttgcggg gtgaggccat ctcgctccgg cgatcggtat ggcccctgcg ggagacggcc
     2101 agtcgactcg atcggctgga aacgcccctg atcgatgccc agtgcggggt gtacttccgc
     2161 gacctctatg accatacggt tcaggtggtt gaggcgaccg aggcgttc
//
LOCUS       DAOSPL010000843         2208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_49376_length_2208_cov_2.819786, whole genome shotgun sequence.
ACCESSION   DAOSPL010000843 DAOSPL010000000
VERSION     DAOSPL010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49376_length_2208_cov_2.819786"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PLQ54_14340"
     CDS             complement(<1..433)
                     /locus_tag="PLQ54_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94496.1"
                     /translation="MLCLLCCLVGQTPALPPSVGFVSHFPMVPTGQAEYGASGATVAL
                     VRAPWALMEPNAGKWDASIIDSQLAWARDQHVRLIYVIECGPAHCVNWVREEARAADG
                     LMKDAAGNTGDPSMFCPTYVERMDAFIRRCVREILDRDQDAT"
     gene            complement(660..1688)
                     /locus_tag="PLQ54_14345"
     CDS             complement(660..1688)
                     /locus_tag="PLQ54_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS94497.1"
                     /translation="MVAPVSPDVLIEAAGLSRHYAIRRGVMRRMRGCVRAVDNVSLVL
                     HRGETVGPVGESGCGKTTLGRCLVRLIEPTEGTVTLHLDGRRIDLLSLNKRELRSIRW
                     AMQMVFQDPQSSLDPRMTIGEIVSEPLVVNRICRGRALEERVAELLVRVGLQPYHMRR
                     YPHAFSGGQRQRIGIARALAVQPKLIVADEPVSALDVSIRAQILNLLADLRNEFGLSY
                     LFVAHDLSAVAYLSDRVAVMYLGSIVELAASEAVYRTPLHPYTEALLSAIPVPDPAVE
                     DARQAVRPVGEPPDPANPPPGCPFHPRCPYAEGRCRELRPDLGELAPGHSVACHRAAD
                     LRLRGIPE"
     gene            complement(1695..>2208)
                     /locus_tag="PLQ54_14350"
     CDS             complement(1695..>2208)
                     /locus_tag="PLQ54_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS94498.1"
                     /translation="YPHQLSGGMRQRAMIAMALSCDPCLLIADEPTTALDVTVQAQIL
                     DLIARAQAERHMALLLITHDLGVVAHMAAAIAVMYLGTVVECGPRNAIFSSPLHPYTQ
                     ALLRSVPVLGRRAKERLSPVRGTVPDPLAVPAGCPFAPRCPEQMEVCCVRPSLQEAEP
                     GHQVACWLHG"
BASE COUNT          370 a          739 c          769 g          330 t
ORIGIN      
        1 ccgtggcgtc ttggtcgcgg tcgagaatct cgcgaacgca gcggcggatg aaggcatcca
       61 tccgttcgac atatgtcggg cagaacatgg agggatcgcc cgtgttgcca gcggcgtcct
      121 tcattagccc gtccgcggcc cgggcttcct cgcgcaccca gttcacacag tgcgcgggcc
      181 cgcactcgat cacatagatg agtcggacat gctggtcgcg ggcccaggcg agctgcgagt
      241 cgatgatgct cgcgtcccac ttcccggcat tgggctccat aagcgcccac ggtgcccgca
      301 cgagggccac cgtggcaccc gaggcaccat actcggcctg gccggtgggg accatgggga
      361 agtggctgac gaagcccacc gatggaggca gggccggggt ctggccaacg agacaacaga
      421 gcaaacagag catgacggga ccccctgaac cgtgttcgat ctgggcagtc gtcccgatta
      481 tgcacgatgc gcgtcggagc gtggcgttct ggccgacaag cgggtgccgc acggccgaga
      541 gctggtgacg atcaccagtg gtgccctctg aggcggtcag gtctcaagcg ggtgccattc
      601 gtgaccgtca ccggtttccc agccgcaggg cgagccagag cccgcgggcg cattccgccc
      661 tactccggta tcccccgcaa ccggaggtct gccgcccgat ggcacgcgac gctgtgcccc
      721 ggcgcgagct ccccgagatc cggccggagc tccctgcacc gcccctcggc gtaggggcac
      781 cgcgggtgga aagggcatcc gggtggaggg ttggccggat cgggcggttc gccgaccggc
      841 cgcaccgctt gccgcgcatc ctcgacggcc gggtcgggga ccgggatggc ggagagcagg
      901 gcctctgtgt atgggtgaag gggtgtgcgg tacacggcct ctgaggccgc gagttccacg
      961 atgctgccga gatacatgac ggcgacacgg tcgctcagat aggccacggc gctgaggtcg
     1021 tgagcgacga acagatagga gagtccgaac tcattccgca gatcggcaag caggttgaga
     1081 atctgcgcgc ggatggacac gtcgagcgcg ctcacaggct cgtcggccac gatgagcttg
     1141 ggctgcacgg ccaaggcccg agcgatgccg atccgttgcc tttgcccgcc gctgaaggcg
     1201 tgcgggtacc gccgcatgtg gtagggctgc aggccgacgc gcacaaggag ctcggccaca
     1261 cgctcctcca gcgctcgccc gcggcagatg cggttgacga ccaacggttc actcacgatc
     1321 tcgccgatgg tcattcgcgg atccagcgat gattgggggt cctggaacac catttgcatg
     1381 gcccagcgga tgctgcggag ctcacgcttg ttcagggaca acaggtcgat ccggcggccg
     1441 tcgagatgga gggtcacggt gccctctgtg ggctcgatca ggcggaccag gcatcggcct
     1501 aaggtcgtct tgccgcagcc gctctcgccc acgggaccca cggtctcgcc ccggtgcaac
     1561 accaggctca cgttgtcgac cgcgcgcacg cagccccgca tccggcgcat cacgccgcgg
     1621 cggatggcgt agtggcggct caggccggcc gcctcgatca gcacatcggg cgagacaggt
     1681 gccaccattt cctcctaccc gtgcagccag caggccacct ggtggccggg ctccgcctcc
     1741 tgcaggctcg gccgcacgca gcagacctcc atctgctcag ggcaccgcgg cgcgaaagga
     1801 cacccggccg gaacggccag cgggtcgggc accgtgccgc gcaccggaga cagccgctcc
     1861 ttggcccggc ggcccagcac gggcaccgag cgcaacaagg cttgcgtgta tggatgaagc
     1921 gggctgctga agatggcgtt ccttggacca cactcgacaa cggtgccgag atacatcacc
     1981 gcgatcgcgg ccgccatgtg ggcgacgacg cccagatcgt gggtgatgag cagcaaggcc
     2041 atgtggcgct cggcctgggc gcgggcgatg aggtcaagga tctgggcctg caccgtcaca
     2101 tccagcgccg tcgtcggctc gtcggcgatc agcaggcacg ggtcgcacga gagcgccatc
     2161 gcgatcatcg ctcgctgcct catgccgccc gagagctggt gcgggtac
//
LOCUS       DAOSPL010000844         2207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_49411_length_2207_cov_2.715613, whole genome shotgun sequence.
ACCESSION   DAOSPL010000844 DAOSPL010000000
VERSION     DAOSPL010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49411_length_2207_cov_2.715613"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PLQ54_14355"
     CDS             <1..400
                     /locus_tag="PLQ54_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94499.1"
                     /translation="PLRGTIEPTCRVAPDATRVTAGGRVRARITVTAGSRHCPGVSVR
                     VDSPAGLVPAEGARNELLRQGAHSARVAADGAWVAIGVGDLAPGEVRQFEVTLVARSP
                     ARVAAVRARVRCESLPPRSPWPAGSLVGPV"
     gene            862..>2207
                     /locus_tag="PLQ54_14360"
     CDS             862..>2207
                     /locus_tag="PLQ54_14360"
                     /inference="COORDINATES: protein motif:HMM:NF018357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HOS94500.1"
                     /translation="MRQPSAKESQVAHGVRRKLIEVALPIEAINAAAAHEKSVPRRGH
                     PATVHLWWARRPLAVCRAVLFAQLVDDPSERPDEFPTLGSQEAERARLLNLVAELSSW
                     RSATDAKLLADARAEILRSCDGSPPTVLDPFCGGGSIPLEAHRLGLNAVGTDLNPVPV
                     LITKSLIEIPPRFAGRAPVRHGANAPAPWPQASALADDTRHYGRWMRERASERIGHLF
                     PGAVIGDETVPVVGWLWARTVPCPNPACGAQAPLVRSFCLSSKHAKPAWVQPVLDGKG
                     GYGFRIGAESGCAPAGTISRLGGHCLVCNAPLSLAHVRAEAQAGRMGTRLMCTIAEGR
                     GRRIYLAPDPTHEEAAGLAQPTWRPDRPLEGKCRVSMPLYGMETFGDLFSPRQLVALE
                     TFSSLVGEARRLAIADAAAVGRCEADARAYGDALAIYLARVGHRVGCRRDPGAQLWL"
BASE COUNT          333 a          781 c          759 g          334 t
ORIGIN      
        1 tccgctgcgc ggcacgatcg agcccacatg ccgagtggcg cccgacgcga ctcgcgtcac
       61 ggctggtggg cgtgtgcggg ctcggataac ggtgaccgcc ggttcgcgcc attgtcccgg
      121 tgtctcggtg cgggtcgact caccggccgg actcgttccg gctgagggtg cacgcaacga
      181 gctcctgcgc caaggcgccc actcagctcg tgtggccgcc gacggggcgt gggtcgccat
      241 cggcgtaggt gatctggccc cgggcgaggt ccgtcagttc gaggtgactc tggtggcccg
      301 gtcaccggcg cgggtggccg ccgtgcgagc cagggtccgt tgcgagtcac tgccgcccag
      361 atcgccatgg cccgcgggat cgctcgtggg gcccgtctga cccccggaat cagaagcgcg
      421 gagccggcga accggctccg cgcgagggtg actgtatcgg attggaggtc accgtcgaga
      481 tcgcaggcta gctcctacag ccacctcgac cggtcatgtc agtactaatc cagaccaccc
      541 ccttccccag tctgaacgct agggtagggc gtcgcgaacg gccgtgtcaa gacggccggc
      601 caccgctgca gggcgttcat actcctcccg caaaccatga accgcggcgc gcacgaatgg
      661 ccttgggccg gaaagggtcc caccggcctg cgactggtcc tccccaatca gtcattccga
      721 ggattgccca agcgactcgg gccgtgccgg gccgcgaagg ctcgccgagg aggaacggcg
      781 ccgtccggca cacactggcg aacgcgatgc cccgggcccc ggtgcggagt tggcttcgcg
      841 cgcccaagat gagctaccct gatgcgacaa ccatctgcca aggagagcca agtggcccac
      901 ggggtacggc ggaagctcat tgaggtggcc cttcccattg aggccatcaa cgcagctgcc
      961 gcgcacgaga agtcggtgcc gcgccgcggg catccagcca ccgtgcactt gtggtgggcg
     1021 cgccgtccgc tcgccgtctg ccgggccgtg ctgttcgccc aactggtcga cgacccgtct
     1081 gagcggcccg acgagttccc gacactcggg agccaggagg ccgagcgagc acggctgctg
     1141 aaccttgtgg ccgagctctc atcgtggcgt agcgccaccg atgcgaagct tctggcggac
     1201 gcccgagccg agatcctccg gagctgcgac ggaagtcctc ccacggtgct ggaccccttc
     1261 tgcggcggcg gttccatccc cctggaagcc caccggctgg ggctgaacgc ggtggggaca
     1321 gacctgaacc ccgtaccggt gctgatcacc aagtcgctga tcgagatacc cccgcgtttc
     1381 gccgggcgag cacccgtgcg acacggcgcg aacgcgcccg ccccctggcc acaggcatcg
     1441 gccctcgcag acgacactcg gcactacggc cggtggatgc gcgaacgcgc ttccgagcgc
     1501 attggccacc ttttccccgg agccgtaatc ggcgacgaaa cggtgccagt ggtcggctgg
     1561 ctttgggcgc ggacggttcc gtgcccgaac ccagcatgcg gcgcccaggc gccgctcgtc
     1621 cgcagctttt gtctttcgtc gaagcacgcc aagccggcgt gggtgcagcc cgtgctcgac
     1681 gggaagggcg gctatggctt ccgcatcggc gcggagtcag gctgcgcgcc agcgggcacg
     1741 atcagccgcc tgggcgggca ctgcctcgtc tgcaatgcgc ccctctcact ggcccacgta
     1801 cgggccgagg cacaggccgg ccgcatgggc acccggctga tgtgtaccat cgccgagggg
     1861 cgaggccgga ggatctacct ggcgcccgat ccgacccacg aggaagctgc gggcctggcc
     1921 cagcccacat ggcgacccga tcgcccactc gagggcaagt gccgggtgag catgccgctc
     1981 tacggcatgg agacattcgg cgacctcttc agcccgcggc agctcgtggc cctcgagacg
     2041 ttcagcagtc tggtgggcga agcccgcaga cttgccatcg ccgatgccgc ggccgtgggg
     2101 cggtgcgagg ccgatgcgcg agcatatggc gatgccctcg ccatctacct cgcgcgcgtc
     2161 ggtcatcgcg tcggttgccg ccgcgatcct ggcgctcagc tatggct
//
LOCUS       DAOSPL010000845         2204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_49494_length_2204_cov_2.949744, whole genome shotgun sequence.
ACCESSION   DAOSPL010000845 DAOSPL010000000
VERSION     DAOSPL010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49494_length_2204_cov_2.949744"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="PLQ54_14365"
     CDS             complement(<1..1188)
                     /locus_tag="PLQ54_14365"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOS94501.1"
                     /translation="MWFLVLAPSLVLPAGEWPQAGDPMVVTCGNDVGWQVVSPEAARA
                     SFLERGELRRDTGAVEEHWARYGHLYGEHGVTAFEPYVKWMLMEPREGEFDPSFYDAE
                     LAAFKAHGLKWVPFLVAGPAYATPPWLKESDRSVFAVDIATGHVSRDQSIWNPHLRPL
                     VRAWLERFFAHYVHDDMQAVLLGISGVFGESIFTAGGNAWTQIWDGEYPQHFGWWCGD
                     EYARASFRAAMSAKYATIADLNAAWQTDLAGFDEVEPFVPDVNRSDRARLDMIRWYMQ
                     SMTDYAEWWVSTTRELAPTVPVLLCTGGGAEPQLGADMSAQTKMVARYGAGMRITNEA
                     SDYAANFHITRHISSAARLYGTSKPAAIHWGVPIDMTPGIAALCHAIAAMWALTGNLD
                     IPGG"
     gene            1370..>2204
                     /locus_tag="PLQ54_14370"
     CDS             1370..>2204
                     /locus_tag="PLQ54_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008200174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOS94502.1"
                     /translation="MDRRTFLGTAAKSLAAASVSFTHLARADSARARRRPNIILILAD
                     DLGYGDLSCFGHPTLHTPHLDRMAAEGMKLTQFYSAAPVCTPSRAALLTGRLPIRSGM
                     YGDRRGVLFPDSVGGLPDSEVTIADALRAAGYATACVGKWHLGHRPQYLPTRHGFDEY
                     FGIPYSNDMKPCPLLDGESPIEEPAVQTTLTRRYTERAVQFIERSRRRPFFLYFAHTF
                     PHVPLHRSTEFAGTSLRGLYGDVVRELDWSVGQIRATLTRLGLHRDTLVLFTSDNGPW
                     LT"
BASE COUNT          368 a          805 c          666 g          365 t
ORIGIN      
        1 tccgccgggg atgtccaggt ttccggtgag ggcccacatg gcagctatgg catgacagag
       61 ggctgcgatg ccgggagtca tgtcaatggg caccccccag tggatggcgg caggcttgct
      121 cgtgccgtag agacgcgcgg cactgctgat gtgccgcgtg atgtggaagt tcgccgcata
      181 gtccgaggcc tcgttcgtga tccgcatccc ggccccgtag cgcgcgacca tcttggtctg
      241 ggccgacatg tccgcgccca gctggggctc ggccccgccg cccgtgcaga gcagcacggg
      301 gaccgtggga gctagttcgc gtgtcgttga cacccaccac tcggcatagt ccgtcatcga
      361 ctgcatgtac cagcggatca tgtccagtcg cgcgcggtcc gaccggttga catcgggcac
      421 gaaaggctcg acttcgtcga agccggccag atccgtctgc caggcagcat tcaggtcagc
      481 gatcgtcgca tacttcgcac tcatcgccgc gcggaagctc gcccgtgcat actcatcccc
      541 gcaccaccaa ccgaagtgct ggggatactc gccatcccag atctgagtcc acgcgttccc
      601 accagccgtg aagatcgatt ccccaaagac tccgctgatg cccagcagta cagcctgcat
      661 gtcatcgtga acgtagtgag caaagaagcg ctcgagccac gcccggacca gcggacgcag
      721 gtgcgggttc cagatcgact ggtcgcggga cacatgaccc gtcgcgatgt ccaccgcgaa
      781 caccgatcgg tcggactcct tcagccacgg gggtgttgcg taggccggtc cggcgaccag
      841 gaagggcacc cacttcaggc cgtgcgcctt gaacgccgcg agctccgcat cgtagaagct
      901 cgggtcaaac tcgccctccc tgggctccat gagcatccac ttcacgtagg gctcaaatgc
      961 ggttacacca tgctcaccgt acaggtgacc gtaccgggcc cagtgttcct caacggcccc
     1021 ggtgtcacgt cgcagctccc cccgctcgag gaatgacgca cgggcagcct ctgggctcac
     1081 cacctgccag cccacatcat ttccgcacgt cacgaccatg gggtcgccgg cctggggcca
     1141 ttcgcccgcc ggtaacacca gactcggtgc cagcaccagg aaccacatcg ctttacctcc
     1201 cgctccccgg gcggctctcg agccccgggt tggtcctgcc agttctcgtg atcgccggct
     1261 ggtgccttct caggcgaagg ccggcccgaa gcgccacagg acatccgttc gcgccgacga
     1321 aaggggccac gctaagcaac cgcgcacgcc gcgccgatcg gaggctccca tggatcgccg
     1381 cacgttcctc ggcaccgccg cgaagagctt ggccgccgcg tcggtaagct tcacgcacct
     1441 ggctcgggcg gactccgcac gggcgcggcg ccggcccaac atcatcctca tcctcgcgga
     1501 cgatctgggc tatggcgacc tctcgtgctt cggccatccg accctgcaca cgccccacct
     1561 ggaccgaatg gccgcagagg ggatgaagct cactcagttc tactccgccg caccggtgtg
     1621 cacgccgagc cgcgccgcct tgttgaccgg ccgtctgccc atccgcagcg gcatgtatgg
     1681 cgaccgccgt ggcgtgctgt tcccggactc ggtcggcggc ctgcccgaca gcgaagtcac
     1741 catcgccgac gcgctccgcg cggcgggcta cgccaccgcg tgcgtgggca agtggcacct
     1801 ggggcaccgc ccgcagtacc tgcccacccg gcacggcttc gacgagtatt tcgggatccc
     1861 ttatagcaac gatatgaagc cctgtcctct gctggatggc gaatcgccca tcgaggagcc
     1921 cgccgtgcag accacgctca cccggcgcta caccgaacgc gccgtgcagt tcatcgagcg
     1981 gtcccggcgg aggccattct tcctctactt cgcccacacg ttcccgcacg tccccctgca
     2041 ccggtccacc gagttcgccg ggacgagtct ccgcggtctc tatggcgacg tggtgcggga
     2101 actggattgg agtgtgggcc agatccgtgc cacactgacc cgcctgggac tccatcgcga
     2161 cacgctggtc ctcttcacca gcgacaacgg cccctggctg accc
//
LOCUS       DAOSPL010000846         2203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_49519_length_2203_cov_3.826350, whole genome shotgun sequence.
ACCESSION   DAOSPL010000846 DAOSPL010000000
VERSION     DAOSPL010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49519_length_2203_cov_3.826350"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="PLQ54_14375"
     CDS             complement(<1..1314)
                     /locus_tag="PLQ54_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94503.1"
                     /translation="MTDYRARQRGSRGQVLGGALLALIIVASAGAVLVALGEQSVRAA
                     ERSKRRLVSGFRADAGLVAARLELEQAMVDSDGSDARDGATAQGEVPSFLNDGDARWE
                     WEVRVIRDGAYWRTTKFIRGYGSYRGQSQVVWERQLRSYSLLNYSWFTHTGSQSFGVA
                     FRSNGAIYAGGDLNFNHNANQYFGSRVEASGGLLNRGNDESESRTFALASMPRSGVPQ
                     VVLDTDVDWFQNTYDTASHGGIVLPTGYDYAIDFTGADLTQAGTLTIKRRARQSTDNS
                     LSTVRTSNSGTWTDYTMTIPSNFNGVIFAGAVGSNGNPTAYSSVKVKGTLQRMSTTVV
                     ATDDAFIWGNTYGGTTNGDFNRDGRHPENDSAGTGDPVNLALVAMHHIRIAKDAPRIL
                     EFEVASCAVEGTIKEEYYNSSFNDSDTNCNPIVSPYDANGTLTGWD"
     gene            complement(1332..2087)
                     /locus_tag="PLQ54_14380"
     CDS             complement(1332..2087)
                     /locus_tag="PLQ54_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94504.1"
                     /translation="MIEALTVIGVIALIGGAILSMLTQASHTYAATVMNGVAQREASS
                     ITDLVRDLVRMSVEITTNREDEIKFQLVPWDTTEGAAKLQPTNDTDYGPEVWIHLADS
                     HGSQGTTGTNLWLAVKQRGASGYGQKTLLSSRIEDFELAYFYAENTTDSGPIYFQIPA
                     GTPQRDMRRRINAVRVRITAFGSDVSDPSLGGAASYSRFTTSTVVYLRNAKYMPGAYQ
                     LLDPNNSDVDERPRTYLVDHGLGQSLGLIPLSP"
BASE COUNT          342 a          709 c          715 g          437 t
ORIGIN      
        1 gtcccagccc gtgagcgttc cattggcgtc atacgggctg acgatcgggt tgcagttggt
       61 gtcggagtcg ttgaagctgg agttgtagta ctcctccttg atggttccct caaccgcgca
      121 ggacgcgacc tcgaactcga ggatacgtgg cgcatccttg gcgatgcgga tgtggtgcat
      181 ggccaccaga gccaggttca cgggatcgcc cgtgcccgcg ctgtcgttct ccgggtgccg
      241 cccgtcgcgg ttgaagtccc cgttggtggt gccgccatag gtgttgcccc agatgaacgc
      301 atcgtcggtg gccaccaccg tggttgacat gcgctgcagg gttcccttga ccttgacgct
      361 gctgtatgca gtcgggtttc cgttgctgcc aacggccccg gcgaagatca ccccgttgaa
      421 gttgctcggg atggtcatgg tgtagtcagt ccaggtaccc gaattgctcg tccggacggt
      481 gctgagcgag ttgtccgtgg actggcgagc acgtcgcttg atggtgagtg tgcccgcctg
      541 ggtcagatcg gcccccgtga agtcaatggc gtagtcgtac cccgtcggaa gtacgatccc
      601 gccatggctg gccgtgtcgt aggtgttctg gaaccagtcg acgtcggtat cgagcaccac
      661 ctggggtacc ccggaccgag gcatactggc gagagcgaac gtgcgtgact cactctcgtc
      721 attgccgcgg ttgagcaggc ctccgctcgc ctcgacccgt gatccgaagt actggttcgc
      781 gttgtggttg aagttcaggt cgccgcccgc gtagatggcc ccattgcttc ggaaggccac
      841 accaaacgac tggctgcccg tgtgggtgaa ccatgagtag ttgagcaggc tgtaggatcg
      901 cagctggcgc tcccagacaa cctgactctg accgcggtag ctcccgtagc cccggatgaa
      961 cttggtggtt cgccagtagg cgccgtcacg gataacgcgg acctcccact cccaacgggc
     1021 gtcgccgtcg ttgaggaagc tcgggacctc accctgcgcc gtggcgccgt cgcgtgcgtc
     1081 gctgccgtcc gagtcgacca ttgcctgttc gagctcgagg cgggctgcga ccagtcccgc
     1141 gtcggcccgg aagccggaga cgaggcgacg cttgctccgt tcggccgcgc ggaccgactg
     1201 ttcgcccagc gcgaccagca ccgctccggc gctggcgacg atgatgagcg ccaggagggc
     1261 gccgcccagc acctggcccc tcgagccgcg ctgtctcgca cgatagtcag tcatggatct
     1321 cacctcctgc ctcaaggaga gagcggaatc aggcccagcg actggccgag gccgtgatcc
     1381 accagatagg tgcgtggacg ctcgtccacg tccgagttgt tggggtccag cagctggtag
     1441 gcgccgggca tgtacttcgc gttcctcagg tagaccacgg tcgatgtggt gaagcggctg
     1501 tagctggcgg ccccacccag cgacgggtca ctcacatcgc tgccaaacgc cgtgatcctg
     1561 acacggaccg cgttgatccg tcgccgcatg tcgcgctgcg gtgtgccggc cgggatctgg
     1621 aagtagatgg gaccggagtc cgttgtgttc tccgcgtaga agtacgccag ctcgaagtcc
     1681 tcgatccgcg acgacaggag cgtcttctga ccgtaccccg aagcgcctct ttgcttgacc
     1741 gccagccaca gattcgtccc ggtggtaccc tgggacccgt ggctgtcggc cagatggatc
     1801 cacacctccg gcccgtagtc ggtgtcgttg gtcggctgga gcttggccgc gccctccgtg
     1861 gtatcccacg gcaccagctg gaacttgatc tcgtcttcgc gattggtcgt gatctcgaca
     1921 gacatccgca ccaggtcgcg gacaaggtcg gtgatggagc tcgcctcgcg ctgcgccacg
     1981 ccgttcatta ccgtggcggc gtaggtgtgc gaggcctggg ttagcatgga caggatcgcg
     2041 cctccgatca gcgcgatgac tccgatgacc gtcagggcct caatcatcgt gaagcctctc
     2101 gttgctcggc acaccatggt ggctcccccc ttcggagccg gcctcgctca ctcgtccctg
     2161 gacaccagag tgtccaggac caggctgccc ttgcttcgct cat
//
LOCUS       DAOSPL010000847         2203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_49524_length_2203_cov_3.397114, whole genome shotgun sequence.
ACCESSION   DAOSPL010000847 DAOSPL010000000
VERSION     DAOSPL010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49524_length_2203_cov_3.397114"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PLQ54_14385"
     CDS             <1..326
                     /locus_tag="PLQ54_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94505.1"
                     /translation="IEHRRRAEDVALDQPFDVIVRATHTGDAPPAEGLLVRPLATEAV
                     TVSQEAIRLGDLNPGGTAQCTFSAVVHERNPERANRFMLAFRGQTTTEGVWPHATAFA
                     YVWVP"
     gene            complement(308..1246)
                     /locus_tag="PLQ54_14390"
     CDS             complement(308..1246)
                     /locus_tag="PLQ54_14390"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HOS94506.1"
                     /translation="MAEVVAVCFRLAPKPYYFDPGDLKLRLGDRVVAETAKGIELGRV
                     TEGRHSVPDELIPQPLKPLLRVATPGDLRKEESNRAKEIEARQACVALIQQHGLPMRL
                     VNVAYTLDRSRVVFYFSAEGRVDFRDLVRDLARSLRTRIELHQVGVRDQAKMVGGFGT
                     CGRPLCCATFLQEFCPVAIRMAKEQGLSLNPTKISGMCGRLLCCLRYEYDTYRELRRT
                     LPKPGTTVETVDGPGVVVDVELLRETFEVELHSAEDDGPPEGNGEVRRYRLSDLVEPE
                     PAPKKRPRKDRRRRRRGRKPKDAGAAEGSPAQGTQT"
     gene            complement(1257..>2203)
                     /locus_tag="PLQ54_14395"
     CDS             complement(1257..>2203)
                     /locus_tag="PLQ54_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOS94507.1"
                     /translation="YDVLVCTTIIENGLDVPNVNTIIVHDADRFGLAQLYQLRGRVGR
                     SSRQAYAYFLYRKPEALTPEAQDRLAALKEFTELGSGLKIAMRDLEIRGTGNLLGPEQ
                     HGYIEAVGFELYCQMLSEAVRQLKGEAVERRELPAVELPLDAYIPESYVQIEGHRLDL
                     YRRISLVRDTDALRQIQDEMVDRFGALPPETENLMRVLSFRLTCAERGITQVMVESGA
                     VVLRVADDRAVTLKSKRVIAKYMLAHRRQTLRDVVVERRRVVVRFMNISMSERLRALD
                     ELVQLVLTSAANGGLGGSSPASRLGGVPVAGARPPRRT"
BASE COUNT          356 a          788 c          688 g          371 t
ORIGIN      
        1 taatcgaaca tcgacgacgg gctgaagacg ttgcgctgga ccagccgttc gatgtgatcg
       61 tgcgggctac ccacacgggc gatgccccac cagccgaggg gctcctagtc cgtcccctgg
      121 cgaccgaggc cgtcaccgtg agccaagagg cgattcgcct cggcgacttg aaccccggcg
      181 gcacggccca atgcaccttc tcggcggtcg tgcatgagcg caacccggaa cgcgccaatc
      241 ggttcatgct cgcattccgt ggccagacca caaccgaggg tgtctggccc cacgccacgg
      301 cgtttgccta tgtctgggtg ccctgagcgg gggagccctc ggccgcgcca gcgtccttgg
      361 gctttcgccc gcggcggcgt ctccgccggt ccttgcgggg ccgcttcttc ggcgccggct
      421 ctggctccac caggtcgctc aatcgatagc gccgaacctc accattgccc tcgggtggcc
      481 cgtcgtcttc cgccgagtgc agctcgacct cgaaggtctc ccggagcagt tcgacatcca
      541 cgacgactcc cggcccgtcc acggtctcga ccgtggttcc cggcttgggc agcgtcctcc
      601 gcagttcacg gtacgtgtcg tactcgtagc gcaggcagca cagcaggcgg ccgcacatgc
      661 ctgagatctt ggtcgggttg agggaaagcc cctgctcctt ggccatccgg atcgcgaccg
      721 gacagaactc ctgcaggaag gtggcacagc acaggggtcg gccgcatgtg ccgaacccgc
      781 ccaccatctt ggcttggtca cgcacgccaa cctggtggag ctcgatgcgc gtgcggagtg
      841 atcgcgccag gtcccggacc agatcgcgga agtccacccg gccctcggcg ctgaagtaga
      901 acaccacgcg cgaccggtcc agggtgtagg ccacgttgac cagccgcatc ggcagcccat
      961 gctgctggat tagcgcgacg cacgcctgcc gggcttcgat ctccttggcg cgattcgact
     1021 cctccttgcg caggtccccc ggcgtggcca cgcggagcag tggtttcagc ggctgcggaa
     1081 tcagttcatc ggggaccgag tgccgcccct cggtgacacg ccccagctcg atgcccttgg
     1141 ccgtctcggc caccacgcgg tcccccaggc gcagcttcag gtcgccggga tcgaagtagt
     1201 aaggcttcgg cgccaagcgg aagcatacag cgacgacctc tgccatagag cccctctcag
     1261 gtcctccgcg gcggccgggc ccccgcgacg ggcacgcctc ccaggcggct tgctggggaa
     1321 gagccgccga gcccaccatt agcggcgctg gtcagcacga gctggacgag ttcgtctagg
     1381 gcgcgaagcc gctcggacat ggagatgttc atgaagcgta ccacgacccg gcgtcgctcg
     1441 acaaccacgt cgcgaagcgt ctggcgccgg tgtgcgagca tgtacttcgc aatcacacgc
     1501 ttgctcttca gcgtaacggc ccgatcgtcg gccacccgca gcaccacggc cccactctcg
     1561 accatcacct gcgtgatgcc tcgctccgcg caggtcaggc ggaagctcag cacccgcatc
     1621 aggttctctg tctccggagg cagcgccccg aagcggtcga ccatctcgtc ctgaatctgc
     1681 cggagcgcat ccgtatcccg caccagcgag atgcgccggt acagatcgag ccgatggcct
     1741 tcaatctgta cgtagctctc cgggatgtac gcgtccagcg gcagctccac cgccgggagc
     1801 tcccgccgct caacggcttc ccccttgagc tgacgcacgg cttccgacag catctggcag
     1861 tacagctcga agcccaccgc ctcgatgtac ccatgctgtt ccggccccag taggttcccc
     1921 gttccgcgga tctccaggtc gcgcatggcg attttgaggc ccgaacccag ctcggtgaac
     1981 tccttgaggg ccgccaggcg gtcctgagcc tcgggcgtca acgcctccgg cttgcggtag
     2041 aggaagtacg cataggcctg gcgactcgat cgcccgacgc ggccccggag ctggtagagc
     2101 tgcgcgaggc cgaagcgatc ggcgtcgtgc acgatgatcg tgttcacgtt cggcacgtcc
     2161 aggccattct cgatgatggt cgtgcacacc aacacgtcgt agt
//
LOCUS       DAOSPL010000848         2197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_49716_length_2197_cov_4.688609, whole genome shotgun sequence.
ACCESSION   DAOSPL010000848 DAOSPL010000000
VERSION     DAOSPL010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49716_length_2197_cov_4.688609"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLQ54_14400"
     CDS             complement(<1..317)
                     /locus_tag="PLQ54_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94508.1"
                     /translation="MARVRRVMRLLPLASIAVAGALFLAARAFLELPHGTILRAVVVQ
                     GVNLGGLTEPQARDTLDSLADSLARRTVLLSLPGGTVPVTLGELGVEPDVDGAVALAL
                     AAGR"
     gene            817..1662
                     /locus_tag="PLQ54_14405"
     CDS             817..1662
                     /locus_tag="PLQ54_14405"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOS94509.1"
                     /translation="MDLSRLPVISAVVREVLELTASPDVAPMAVAQAIEGDPVVCARL
                     LRVANSPYFGAARAIGSVRHAVTFLGLEMVRALVVSAAVMEALRPTRPLAGIPLDAFW
                     EHSSAVAVLARRVATSEAPALSGEAFVAGLLHDIGSLILACSSPDRYRQVLETGARKQ
                     WPIVAVEPDAFGMNHAHVGGAAARHWGLPHALVNAIQHHHSPGQALRYGELASVVHVA
                     DAAAQSMGIAGFETAPGPWVEPCAWLILGGRRLGAEADRLSRYTGSQTDQQAIRGLSA
                     ALAAG"
     gene            complement(1800..1961)
                     /locus_tag="PLQ54_14410"
     CDS             complement(1800..1961)
                     /locus_tag="PLQ54_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94510.1"
                     /translation="MKRIVLLGLAVFMLAAPIMAGCKSSDSKKPSPEAPADQQKLGKS
                     LIDQVKKGN"
     gene            complement(2000..>2197)
                     /locus_tag="PLQ54_14415"
     CDS             complement(2000..>2197)
                     /locus_tag="PLQ54_14415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94511.1"
                     /translation="CNGSRACDPSVANVHNETANLLYVDGHVKAKKIPLSVPNVNPAP
                     AGDWRWELDPDNGWYQYNAGT"
BASE COUNT          346 a          743 c          748 g          360 t
ORIGIN      
        1 cgcccggccg ccagggccag cgccaccgcg ccatcgacgt cgggctccac accgagttcg
       61 cccagcgtga ccggcacggt gccaccgggg agactgagca ggacggtccg tcgggccaat
      121 gagtcggcca gcgagtccag ggtgtcgcgt gcctgcggct cggtcaaccc acccaggttc
      181 acgccctgaa cgacgacggc gcggaggatc gtaccgtggg gaagctccag aaacgcgcgg
      241 gcggccagga agagggcacc ggcaacggcg atgctggcaa ggggaaggag gcgcatcact
      301 cggcgcacac gcgccacctc ggactcgtcc gcggcctcct cagtcacagc cggcgagggg
      361 gaggcgccaa cgcccatgct gagccggaga gggcggtcca cagcgcactg acctactccg
      421 aacgtcagcc tagccacccc gtcttcgggt gtcaacccgc acgatcacat caacgaagga
      481 cagtccagtt gggcacctga agatgaaaac gaatggctac ggtcacggga gcggcacgac
      541 gagcctggcc cggggcatcc ccggcatcgt ctctggggaa ccctggccgc gacgcgcgga
      601 cctcggcggg tggcgttcgg ctccgccggt catcggtcag ccatagccat ggagcgggat
      661 ccacagtccg ggggggcgtt cagactggcc ggtccgaatc tgagtgctgc tgcggaacac
      721 ctctgttggg gaacccatgt tccacgccga acggagggag ccgtcttcaa accgacggga
      781 tcaggtgagc agcctcacgg ccgtgcggct gaactggtgg acctgagccg tctgccggtg
      841 atctcggcgg tggtccgtga ggtactggag ctgacagcct cacccgacgt ggcaccgatg
      901 gccgttgccc aagccatcga gggcgatccg gtggtgtgtg cccgattgct tcgcgttgcc
      961 aactccccgt acttcggggc cgcccgggcc atcggctcgg tccgtcacgc cgtcacattc
     1021 ctgggtctcg agatggtccg cgcgctggtg gtcagtgccg cagtcatgga agcgctgcgg
     1081 cccacccggc ccctggccgg catccccctc gatgcattct gggagcacag cagtgcggtg
     1141 gcggtgctcg ctcgccgcgt cgccacgtcg gaggcgcccg ccctgagcgg cgaggccttc
     1201 gtcgcggggc ttctgcacga catcggcagc ctgatcctcg cctgcagctc gcccgatcgc
     1261 taccgccagg tgcttgagac gggggctcgg aagcagtggc ccatcgtggc cgttgaaccc
     1321 gacgccttcg ggatgaatca cgcccacgtc ggcggggccg ccgcgcggca ctggggcctg
     1381 ccccacgcgc tggtgaacgc catccagcac caccactccc ccgggcaggc gctgcgctat
     1441 ggcgagctgg cgtccgtcgt tcacgtggcg gacgctgcgg cccagagcat ggggatcgct
     1501 gggttcgaga cggctcccgg cccatgggtc gagccatgcg cctggctcat cctggggggc
     1561 cggcgcctcg gcgctgaggc cgaccgactg agccgctaca cgggtagcca gacggatcag
     1621 caggcgatcc ggggtctgag tgccgccttg gcggccgggt agcgccgtct cggtctgcag
     1681 atgcatggtc atatgctgat atgctcatat gaccactcat cgatgtacct ggttctcttc
     1741 acagatagac agccgggagc gcgactgatc gccgcgctcc cggctgtccc gctccgggac
     1801 tagttgccct tcttgacttg gtcgatcagg ctctttccga gcttctgctg atcggccggc
     1861 gcctcgggtg acggcttctt gctgtcggac gacttgcagc cggccatgat cggcgccgcg
     1921 agcatgaaca cggccagacc caacaggacg atgcgcttca ctgtgctcaa acctccatca
     1981 tatcccgtga ctcggtcgcc taggttccgg cgttgtactg gtaccagccg ttgtccgggt
     2041 cgagctccca gcgccagtcg ccggccggag cggggttcac gttgggaacg gacaagggga
     2101 tcttcttggc cttgacgtgc ccatcgacat agagcaggtt ggcggtctcg ttgtggacat
     2161 tagccacgct cgggtcacag gcccgagagc cgttaca
//
LOCUS       DAOSPL010000849         2196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_49747_length_2196_cov_3.950023, whole genome shotgun sequence.
ACCESSION   DAOSPL010000849 DAOSPL010000000
VERSION     DAOSPL010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49747_length_2196_cov_3.950023"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLQ54_14420"
     CDS             complement(<1..302)
                     /locus_tag="PLQ54_14420"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOS94512.1"
                     /translation="MSANHDLHTHTTFSDGAHSVALHVLEARAFELDAIAITDHYTPG
                     SALADDATFDRYLAEIGAARQGQDDVIVLKGAETQAMDTRGTVSLDARHAARLEWVL"
     gene            complement(317..1105)
                     /locus_tag="PLQ54_14425"
     CDS             complement(317..1105)
                     /locus_tag="PLQ54_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018977748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOS94513.1"
                     /translation="MALVHYQFFAESLGLCTSAYIALPQPPGPEIAKHGKKAAGPYQT
                     MYLLHGLSDDHSIWLRRTSIERYADAAGIAVVMPEVGRSWYTDMARGLRYWTYVSEEL
                     PSLCQAVFPLSAEREDNYVAGLSMGGYGAFKLALRCSDRFAAGASLSGALDMASSARA
                     EDGEWRRELQWVFGPLDQFAGSDHDLLHLASHVAHSAGPKPRLYQWCGTEDFLYEQNQ
                     RFREHAKTLGLDLTYEESPGGHTWDRWDAKIQRVIEWLGLPKPK"
     gene            complement(1132..>2196)
                     /locus_tag="PLQ54_14430"
     CDS             complement(1132..>2196)
                     /locus_tag="PLQ54_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOS94514.1"
                     /translation="VVSLNEAVAIVTGHGEREPFRLDASETQLDRPAYEADFHDVKGQ
                     EPAKRALEVAAAGGHNCLMVGPPGSGKTMLARRLPSILPPLSLSEALEVTKLYSVAGL
                     LPSDVSLLSVRPFRAPHHTTSTAGLCGGGSIPRPGEVSLAHHGVLFLDELPEYRKDAL
                     EVLRQPLEDRQITISRATASLTYPASFTLVGSMNPCPCGFYGDTVRQCVCNAQQVRRY
                     LDKLSGPMLDRIDIHIEVPRLSEQELMARPTGESSETIRERVLRARAVQLKRFEGRSV
                     FTNASMTSRDLRTYCEPLPEAQALIRAAIAQFALSARAYDRILKVARTVADLAGEESI
                     GVAHIAEAVQYRALDRKLWG"
BASE COUNT          366 a          766 c          685 g          379 t
ORIGIN      
        1 agcacccact cgagccgcgc cgcgtgccgt gcatcaaggc tgactgtgcc gcgggtgtcc
       61 atggcctgtg tctcggcccc tttgagcacg atgacgtcat cctgtccttg ccgcgccgcg
      121 ccaatctcag ccaggtaccg atcgaatgtg gcatcatcgg ccagggccga gcccggagtg
      181 tagtggtcgg tgatcgcaat ggcgtccagc tcgaaggccc gcgcctccag cacatggagc
      241 gccacggagt gcgccccgtc tgagaacgtc gtgtgcgtgt gcagatcgtg gttggcactc
      301 acgtccggcc cctgccctac ttcggctttg gcaaacccag ccactcgatc acacgctgga
      361 tcttggcatc ccaccggtcc catgtgtgcc cgcccgggct ctcttcgtaa gtcagatcca
      421 ggcccagcgt cttcgcgtgc tcgcggaaac gctggttctg ctcgtagagg aagtcttcgg
      481 tcccgcacca ctggtagagc cggggtttcg ggccggccga gtgcgcgacg tggctcgcca
      541 ggtgcagcag atcgtggtcc gacccagcga actggtcgag gggaccgaag acccactgca
      601 gctcgcggcg ccactcgcca tcctctgccc tcgccgaact cgccatatcc agtgcgcccg
      661 agaggctggc accggcggcg aagcggtcgg agcatctcag agccagtttg aatgctccgt
      721 aaccacccat cgatagcccg gccacatagt tgtcctcccg ctccgccgac agcggaaaca
      781 ccgcctggca caacgaaggc agttcctcgc tcacgtaggt ccagtagcgg agcccgcgag
      841 ccatatcggt gtaccagctc cgccccacct ccggcatgac cactgcgatg cccgcggcgt
      901 ccgcgtaacg ctcgatcgag gtgcgccgta gccagatgct atggtcatcg gacaggccgt
      961 ggagcaggta catggtctgg tatggccccg ccgccttctt cccatgcttg gcgatctccg
     1021 gccccggggg ctgaggaagc gcaatatacg ccgacgtgca cagaccaagc gattcggcga
     1081 agaactggta gtgtacgagt gccatggtca ggactcctgt ctgtcgcggg cttaccccca
     1141 cagtttccgg tcgagcgccc ggtactgcac ggcttccgcg atgtgcgcca cgccgatgga
     1201 ctcctcgccc gccagatcgg ccacggtgcg tgccaccttc agaatgcggt cgtaggctcg
     1261 ggcgctcagg gcgaactgcg cgatggccgc ccggatcagt gcctgggcct cgggcaacgg
     1321 ctcgcagtag gtgcgaaggt cccgcgacgt catgctggca ttggtgaaca cgctgcgccc
     1381 ctcgaaccgc ttgagctgga ccgcccgcgc cctcaacacc cgctcgcgga tggtctccga
     1441 ggactcgccc gtcggccgcg ccatcagctc ttgttccgaa agccgcggca cctcgatgtg
     1501 aatgtcgatc cggtccaaca tcggccccga cagcttgtcc agataccgcc gaacctgctg
     1561 cgcattgcac acgcactgcc gcaccgtatc accgtagaag ccgcaagggc aagggttcat
     1621 gctgcccacg agcgtgaacg aggccggata ggtgagtgat gcggtagcac gcgatattgt
     1681 gatctggcga tcctcaagcg gttgccggag cacctccaat gcatccttgc ggtactcggg
     1741 cagttcgtcc aagaacagca ccccgtgatg ggccaagctc acctctcccg gccgggggat
     1801 gcttccgcct ccgcatagac ccgccgtcga ggtcgtgtgg tgcggggcgc ggaacggccg
     1861 caccgacaac agtgacacgt ctgagggcag caacccggcc acactgtaga gcttggtcac
     1921 ctccagagcc tcgctcagcg acagcggcgg caagatgctt ggcaggcgcc gggcgagcat
     1981 cgtcttcccg ctgcccggcg gccccaccat caggcagttg tgtcccccgg ccgcagcgac
     2041 ctccagggct cgcttggcgg gctcctggcc cttcacgtcg tgaaagtctg cctcgtaggc
     2101 cggccgatcc agctgggtct ccgaggcatc caggcggaac ggctcgcgct cgccgtggcc
     2161 cgtgacaatg gccacggctt cgttgaggct gaccac
//
LOCUS       DAOSPL010000850         2195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_49802_length_2195_cov_2.382710, whole genome shotgun sequence.
ACCESSION   DAOSPL010000850 DAOSPL010000000
VERSION     DAOSPL010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49802_length_2195_cov_2.382710"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2195
                     /locus_tag="PLQ54_14435"
     CDS             <1..>2195
                     /locus_tag="PLQ54_14435"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOS94515.1"
                     /translation="GTWNAEWQVSARALDSAWEVALVLPWASLGGAPVEGDEWGFNVC
                     RCRQAEPELSQWSPTVEGFHKPEAFGRIGFGDCPWPVRVHWDLPARTEGRVRIDWQPE
                     GVGPSVALRVNGVETAGAFALPGEGAVPLLCEALAGGVPVFRTLHIAAVTPLQGALDA
                     ARALVGALPESLGPRRAALAAELDHLARLADETPPAMASDLMSHVQRVGLNASHLAVL
                     AAVPEGSIAYGIETSLTKLLRHEPFRGQAGGTLTLDAARREMDARQVVLFAGERPLLR
                     VSAELTKVTSEAGIALPEDAFRIRQVGYVPTVKPSYAVDHVGLWPDPLMPFERLDVRE
                     GGFESLWIDVRVPADAAPGLYRGQLILAAQNASQTAVPVEVRVRRFTIPQAPSVRTAF
                     GLGPGGWRVPQDADAYTRNALEHRISPYTVGAPRLVSPPAMNWEGAERIEVDLHAAGS
                     GILRLAVTPVDGSPVLLDPQPVQAPEMHVTFDVSSVPAQVLSWRLELEAPEVAEAAKA
                     TVRLVRGDGTETLIEDRESRRVLGPDGWLESWAHWEGSAWEDPDVPGVWDWSDFDQQV
                     STYLPLGLTGHVVGLSDPRSAWAREWQAHLQENGWAHLGYTYLFDEPTPDRYPLLNDV
                     MGEVKRAAPGLMNMMTAREFPPELRYVDIWCPEAYSFDPEAARAEQVKGKAVWWYVAF
                     STRHPYPNVWVDYPALDCRVWPWMTWKHDLDGMLYWSAVYWFRNDPWRC"
BASE COUNT          298 a          749 c          788 g          360 t
ORIGIN      
        1 cggaacgtgg aatgccgagt ggcaggtctc ggcgcgggcg ctggactccg cctgggaggt
       61 cgccctggtg ctgccatggg cgtcgctggg tggggcgccc gtcgagggag atgagtgggg
      121 cttcaacgtg tgccgatgcc ggcaggcgga gcccgagcta tcgcagtggt cgcccacggt
      181 cgagggcttc cacaagccgg aggccttcgg ccgcatcggc tttggggact gcccatggcc
      241 ggtgcgagtc cactgggacc tccccgcgcg caccgagggg cgcgtacgga tcgactggca
      301 gcccgaaggt gttggtccca gtgtcgcgct gcgcgtcaat ggcgttgaga cggcgggcgc
      361 ctttgccctc cccggcgagg gggccgtccc gctcttgtgt gaggcgctgg ccggcggtgt
      421 cccggtgttc cgcacgctgc acatcgctgc ggtgacaccc ctccagggcg cgctcgacgc
      481 agcccgcgca ctggttgggg cgctgcccga gagcctcgga ccccggcgcg ccgccctggc
      541 ggccgaactc gaccatctgg ccaggctggc ggacgagaca ccgcccgcca tggcatcgga
      601 tctcatgtcg catgtgcaac gtgtcggtct gaatgcctcg cacctggccg tgctcgccgc
      661 ggtgcccgag ggcagcatcg cctatggaat cgagacgtct ttgaccaagc tgctccgcca
      721 cgagcccttc cgcggccagg cgggtgggac cctcacactc gacgccgcgc ggcgcgagat
      781 ggatgcccgt caggtcgtgc tgttcgccgg cgagcgtccg ctgctgcggg tctcggccga
      841 gctcaccaag gtcacgtccg aggcggggat cgcgctgcct gaggatgcgt tccgcattcg
      901 tcaggtgggc tatgttccca ccgtcaagcc cagctacgcg gtcgatcatg tgggtttgtg
      961 gcccgatccg ctgatgccat tcgagcggct cgacgtgcgc gagggtggct tcgagtcgct
     1021 gtggatcgat gtccgagttc cggccgatgc cgcccccggc ctgtaccgcg gccaactcat
     1081 tctggccgcc cagaacgcct cgcagacggc ggtgcccgta gaggtgcgcg tgcgccgctt
     1141 caccatcccc caggctccga gtgtgcgcac ggcgttcggc ctcggccccg gcggctggcg
     1201 ggtgccccag gacgccgatg cctacacgcg caacgcgctc gagcaccgca tcagccccta
     1261 cacggtgggc gcgcccaggc tggtctcgcc acccgccatg aactgggagg gcgccgagcg
     1321 gatcgaggtc gatctccatg cggccggctc agggatcctc cggctcgccg tgaccccggt
     1381 cgacggcagc cctgtcttgc tcgaccccca accggtgcag gcgccggaga tgcatgtcac
     1441 cttcgacgtc tcttcagtgc cggcgcaggt cctgtcgtgg cggctcgagc tcgaggcgcc
     1501 cgaggtggcc gaggcggcca aagccaccgt ccggctcgtg cgcggtgacg gcaccgagac
     1561 cctcattgag gaccgggaga gccgccgtgt gctcgggccc gacgggtggc tggagagctg
     1621 ggcgcactgg gagggttcgg cgtgggaaga cccggacgtg ccgggcgtgt gggactggtc
     1681 ggacttcgac cagcaggtga gcacgtacct gcctctgggg ctcaccgggc acgtcgtggg
     1741 cctctctgat ccccggagtg cgtgggcgcg cgagtggcag gcccacctcc aagagaatgg
     1801 ctgggcgcat ctcgggtaca cgtacctgtt cgacgagccg acgcccgacc ggtatcccct
     1861 gctgaacgac gtcatgggcg aggtcaagcg cgcggcgccg ggcctgatga acatgatgac
     1921 cgcgcgcgag tttccgccag agcttcgcta cgtcgacatc tggtgtcccg aggcctactc
     1981 gttcgatccc gaggccgccc gcgccgagca ggtaaagggc aaggcggtgt ggtggtacgt
     2041 ggcattcagc acgcgccatc cctatcccaa cgtctgggtc gactacccgg cgctcgactg
     2101 ccgcgtgtgg ccatggatga cctggaagca cgacctggac ggcatgctct actggtcggc
     2161 cgtgtactgg ttccgcaatg acccctggcg gtgcg
//
LOCUS       DAOSPL010000851         2193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_49843_length_2193_cov_3.984097, whole genome shotgun sequence.
ACCESSION   DAOSPL010000851 DAOSPL010000000
VERSION     DAOSPL010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49843_length_2193_cov_3.984097"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            532..1917
                     /locus_tag="PLQ54_14440"
     CDS             532..1917
                     /locus_tag="PLQ54_14440"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOS94516.1"
                     /translation="MSKKAKVISALPVVRRDVAGVDIGSTQHWVAAPQADPDTPNVRV
                     FGSTTGALEELIDWLVSLGVKSVAMESTSVYWIPLYELLEARGLEAVLVNARHLRSVP
                     GRKTDMRDCQWLQTLHSCGLLRGSFRPPEQIARIRAIHRQQGNLVRERTRAVQWMQKA
                     LDQMNVQVHRAVTDITGTTGMAIVRAIVDGVRDPNKLAELRDVRCAKPQAVIAQYLKG
                     TWREEHLFNLGSALRLHDFVTSEIERYDHMLEAELARLESEEHRDQSPPALPSKVKEK
                     KIIREGKGQRRDQLFRVAGVDLTRIDGLGVDAASTIIDELGPDLSAFPTEHHFVSWLR
                     LCPNRAYSAGVPLPKKRQNGAGSSRVGAVLRMAATALARSQTALGAYYRHIAKRKDAA
                     VAAFATARKLACLVYRALRYGTEYTDQGAEAYERAYHERRLKSLKHAAISLGFDLVPA
                     TPTGASAPSAG"
     gene            complement(1978..>2193)
                     /locus_tag="PLQ54_14445"
     CDS             complement(1978..>2193)
                     /locus_tag="PLQ54_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94517.1"
                     /translation="LRRRWRIPEAGWRGLGRRRSDGVRDRIDRRCGSLVDRRLGRLGS
                     RSLSLGRLGSRSLSLCRLGSWSLGRLT"
BASE COUNT          388 a          763 c          723 g          319 t
ORIGIN      
        1 tttgagggct gcgagagcga cgccgcaggc ggatcgagcc gtaagcagcc tcgcaccgca
       61 ggaacgagcg ggaccgacga cgccggcgct cggcgcagcc gcgcgtgccc tccatgcgag
      121 cgcgagcgag ccgagatgcc ggccaggagg cgcccgaagc gagtgacgag acgggcttgc
      181 tgcccgccgc aggaacgagc gagggaccga cgacgccggc gctcggcgca gccgcccgtg
      241 cccacccata cgagcgcgag cgagccgaga tgccggccag tggtgccacc gcccggtggg
      301 cgggtctttc agaccagaaa ctccccttcc tcgtgaggac gagcttcagt ggctccgacg
      361 gtcgaggctc gacagctatc tcccttcctc cattggtgcc tgtgccccac cgcaagtctg
      421 gagccgagct cgcggtcttt cattgatgag gtcgaaagac tctctcccag gttagattct
      481 acgtccgccg cctcgggtcg agcttctacc gtctccacga aggaggtggt catgagcaag
      541 aaggcgaagg tgatctcggc gctgccggtg gttcggcgtg acgtggccgg ggtcgacatc
      601 ggaagcactc agcactgggt cgcggcgcct caggcggacc ccgatacgcc caacgtccgg
      661 gtcttcggaa gcacgaccgg ggcgctcgag gagctcatcg actggctggt gtccctgggc
      721 gtgaaatcgg tcgccatgga gagcacgtcg gtctactgga tcccgctcta cgagctcctg
      781 gaggctcgtg gcctcgaggc ggtgttggtc aatgcgcggc atcttcgcag cgtgccgggg
      841 cgcaagaccg acatgcgtga ctgccagtgg ctccagacgc tgcatagctg cgggctgctc
      901 aggggctcgt tccgtccccc agaacagatc gcgcgcatcc gagccatcca caggcagcag
      961 ggcaacctcg tccgcgagcg gacccgcgcg gtgcagtgga tgcagaaggc gctcgaccag
     1021 atgaacgtgc aggtccaccg ggcggtcacc gatatcaccg ggaccaccgg gatggcgatc
     1081 gtgcgagcca tcgtcgacgg cgtccgagac cccaacaagc tcgccgagct tcgagatgtg
     1141 cgctgcgcca agccacaggc ggtgatcgcc cagtatctga aggggacctg gcgcgaggaa
     1201 cacctcttca atctcggctc cgcgctccgc ctccacgact tcgtgacgag cgagatcgaa
     1261 cggtacgatc acatgctcga agccgaactc gcgcggctcg aatccgagga gcaccgcgac
     1321 cagtcccctc ctgctctccc cagcaaagtc aaagagaaga agatcatccg agagggcaag
     1381 ggccagcgac gagaccaact cttccgagtg gctggggtcg atctgacgcg catcgacggg
     1441 ctcggcgtcg acgccgcctc gaccatcatc gacgagctcg gccccgacct gagcgccttc
     1501 ccgaccgagc atcacttcgt gtcgtggctg cgcctgtgtc ccaaccgagc gtactcggcc
     1561 ggggtaccgc tcccgaagaa gcgccagaac ggcgcgggtt cgagtcgcgt cggagccgtc
     1621 ctgcgcatgg cggccacagc cttggcccgg tcccaaaccg cactcggcgc ctactaccgc
     1681 cacatcgcca agcgcaaaga cgccgccgtc gccgcattcg ccacagcgcg caagctcgcg
     1741 tgcctggtgt atcgcgccct tcgctacggc acggagtaca ccgaccaggg cgccgaggcc
     1801 tatgagcgag cctaccatga gcggcgcctc aagtccctga agcacgctgc catcagcctc
     1861 ggattcgacc tcgtgcctgc gacaccaaca ggcgcgtcgg caccatcggc tggatagcag
     1921 agagccgcta aacggccgca ccagaacagc ttttgttctg gatggttggc gggagtttca
     1981 ggttaggcgg cccaggctcc agctcccaag ccggcagagg ctcaggctcc ggctcccaag
     2041 ccggccgagg ctcaggctcc ggctcccaag ccggccgagg cgccggtcga caaggctgcc
     2101 gcagcgcctg tcgatgcggt cgcgcacgcc gtcgctccgg cgccggccca ggccgcgcca
     2161 gccggcctct gggatgcgcc aacggcggcg cag
//
LOCUS       DAOSPL010000852         2191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_49919_length_2191_cov_3.113764, whole genome shotgun sequence.
ACCESSION   DAOSPL010000852 DAOSPL010000000
VERSION     DAOSPL010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49919_length_2191_cov_3.113764"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..1490
                     /locus_tag="PLQ54_14450"
     CDS             234..1490
                     /locus_tag="PLQ54_14450"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS94518.1"
                     /translation="MGGSDSINRREFLRKSTVAAAGAALLSSTAAASARVVGANDRVH
                     MATIGCGGMGHAHLGALASLRAQGLVDIVSVCDVWDKRVQSAVAATGAKGVRDYRRVL
                     DDPDVDAVSIATPDHWHAPMAIAALDAGKDVYCEKPMTYWKDLSAARAVVDAVARNHR
                     VLQVGTQGMSDSIWEQVGERIKAGAVGQLVHAQASDMRNGPLGVYEPASNDPDAKPGI
                     NLNWNMWLGPAPRRPYDHGRFFAFRSFWDYSGGICTDFFPHTLTPLIRTMGLTFPRRA
                     SASGGLYQLRDGREVPDIFNLTLEYPGGPSVLLIAAVACSQGIPMLIRGHQAAVTFDG
                     PGATIRPEPAVVGNQPTEEVTRERAGSLEAHFEDFIDCVRTRKKPRSHELLGYYVMTA
                     LHMGVRSYLENRVFEFDAQTERVRAV"
     gene            complement(1527..1937)
                     /locus_tag="PLQ54_14455"
     CDS             complement(1527..1937)
                     /locus_tag="PLQ54_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94519.1"
                     /translation="MLVPVRGTLVELMLREYGGDAVLRVASNELAPEFVEGDYVGVCL
                     PMNRPPRPGDAVVVTLGDDPEPARPQFTLRLYDEFAGEPALLPVLRDSDDDLLMDGAA
                     IWAVAVWLYRDTAAAAQQPPSAIEVREEASAPAA"
BASE COUNT          337 a          792 c          748 g          314 t
ORIGIN      
        1 aggggccctg accggcccga ggttggggcg tgcggatcgg gcgcgttggg ggcccctccg
       61 caaccaggct gatgggccac gggagcccca agttaccgcc catgggggcc catgcctccg
      121 ggccccggag cactcgaacc tttccccagg caggactcgc ggcgcgggcc gggaaccagc
      181 ccagagctgg cgcgtcggcg ccggagcggc acgcggaaca gggggcgggt tcgatgggcg
      241 gaagcgacag catcaatcgg cgtgagtttc tcaggaagtc caccgtcgcg gcagcgggtg
      301 ctgcgcttct gagctccacc gccgccgcct ccgcccgtgt ggtcggcgcc aacgaccgcg
      361 tccacatggc caccatcggc tgcgggggca tggggcacgc ccacctgggc gccctggcca
      421 gccttcgggc ccagggactc gtggacatcg tgtccgtgtg cgacgtctgg gacaaacgcg
      481 tgcagtcggc tgtggcggcc acgggagcca agggcgtgcg cgactaccgg cgcgtgcttg
      541 acgacccgga cgtcgacgcg gtgtccatcg ccacacccga ccactggcac gcgccgatgg
      601 ctatcgccgc cctcgatgcg gggaaggatg tctactgcga gaagccgatg acctactgga
      661 aggacctgtc ggccgcccga gccgtggtcg acgcggtggc gcggaaccac cgtgtgttgc
      721 aggtgggcac ccagggcatg tccgacagca tctgggaaca ggtgggtgag cgcatcaagg
      781 caggcgccgt gggccagctg gtgcacgccc aggctagcga catgcggaac ggccccctgg
      841 gcgtctacga acccgcttcc aacgaccccg atgccaagcc gggcatcaac ctcaactgga
      901 acatgtggct cggtccggcc cccaggcggc cctatgacca cggacggttc ttcgccttcc
      961 gctcgttctg ggactactcg ggcgggatct gcaccgactt cttcccccac accctgaccc
     1021 cgctgatccg caccatgggc ctgaccttcc ctcgccgcgc ctcggcctcg ggcggcctct
     1081 accagctccg ggacggccgc gaggtgccgg acatcttcaa cctgacactc gagtacccgg
     1141 gcggcccctc ggtgctgctt atcgcggccg tggcctgcag tcaggggata cccatgctta
     1201 tccgtggcca ccaggccgcg gtcaccttcg atggccccgg agccaccatc cgccccgagc
     1261 cggccgtggt cggtaaccag cccaccgagg aggtcactcg cgagcgcgcc gggtcgctcg
     1321 aggcgcactt cgaggacttc atcgactgtg tccgcacacg caagaagccc agaagccacg
     1381 agctcctggg ctactacgtg atgacggcgc tccacatggg tgtgcggagc tatcttgaga
     1441 accgtgtctt cgagttcgac gcccagaccg agcgcgttcg agccgtgtag ggcgacgggc
     1501 gcccgtgccg gttggctcgg gctcgttcac gcggctggcg cggaggcctc ttcgcgcacc
     1561 tcgatggcgc tgggcggttg ctgggccgcc gcggcggtgt cgcggtagag ccacacggcc
     1621 acggcccaga tggccgcgcc gtccatcagc aggtcgtcgt ccgagtctcg cagcacgggc
     1681 agcaaggccg gctcgcccgc gaactcgtca tacaggcgca gcgtgaactg gggccgcgcg
     1741 ggctcggggt cgtcgcccag ggtgacgacc accgcgtcgc ccgggcgcgg cggtcggttc
     1801 atcggcaggc acacgcccac gtagtctccc tcgacgaact cgggcgccaa ctcgttggac
     1861 gcgacgcgca gaaccgcgtc cccaccatac tcgcgaagca tgagctccac caaggtccca
     1921 cgaaccggaa ccagcaccag gatcacatcc catccgtctc ggttgcctta tcggcagtcc
     1981 ggaccggcaa cttgacggtg ggaatcccca cccgaccagc acccgccggg caacgggttc
     2041 cccagccggc attggcggta actgagggct cccgtggccc atccgcctgg ttgcggaggg
     2101 gcgccatggg gggaaccacg cgggcttcag cgtggcgggc ccccaacgcg cccgatctgc
     2161 acgccccaac ctcgggccgg tcaggggccc c
//
LOCUS       DAOSPL010000853         2191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_49930_length_2191_cov_2.395599, whole genome shotgun sequence.
ACCESSION   DAOSPL010000853 DAOSPL010000000
VERSION     DAOSPL010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49930_length_2191_cov_2.395599"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PLQ54_14460"
     CDS             complement(<1..759)
                     /locus_tag="PLQ54_14460"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOS94520.1"
                     /translation="MRITIAGYSFHGLLAEGRIDVFGYLETVQYRYGIHAADLWNGLV
                     GSVEEGFLRKLRRTLDEREMTVANYHADGCHIWEDDPDARKRNRELAEAHLRAAAILG
                     AETVRIDTGGTQVPFGSEQLDTITRAYQDYCDFGASHGFTVGPENHWGFSLLADNMET
                     LANAVGRPNYGILLHLGHWDDGDEVGGDRRMAPWTAHTHVDARITATRLEEHMGILRE
                     AGYAGYWGVEHHTAKNEYREVAWQLACVQRVLSQW"
     gene            complement(901..2040)
                     /locus_tag="PLQ54_14465"
     CDS             complement(901..2040)
                     /locus_tag="PLQ54_14465"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HOS94521.1"
                     /translation="MSIHRCMLAGTVLAASVVPLAGQGTPSPEISRDAAGNVVLSGPR
                     GCELRYWLIGPGASSLSRRYAGPFDVAAGLAVGAVAIPSDPVDYAARVSPLARDDFGA
                     AGIEPQAEDVGKWAAAWEAFTPDLSGGGVQRVRDDLPLSDQANEGRWVPYEPLTDEFE
                     GDTLDATKWWPNNPGWKGRQPAFFYAGNVTVSDGKLHLAMRKQEVAEASKEEGYHTYT
                     SAAVKSKQTVRYGYFEVKAKPMKSAGSSSFWFYDSTDDWWTEIDVYEIGGRAPGYERK
                     LNMTVHVMRTPAERQHIAIGGAYETPWDLVDDYHVYGLEWDERELRFYLDGVLVRWGP
                     NTHWHQPLTLNFDSETMPDWFGLPKDEDLPSTYSIEYVRAWKRGE"
BASE COUNT          314 a          760 c          716 g          401 t
ORIGIN      
        1 ccactgtgac agcacgcgct ggacgcaggc gagctgccag gcgacttcgc ggtactcgtt
       61 cttggccgtg tggtgttcca caccccagta gcccgcgtag ccggcctcgc gcaggattcc
      121 catgtgctcc tcgagccgcg tggccgtgat ccgggcatcg acgtgggtgt gcgccgtcca
      181 cggggccatg cggcggtctc cgcccacttc gtcgccgtcg tcccagtggc ccaagtgcag
      241 caggataccg tagttcggcc gaccgaccgc attggctaga gtctccatgt tgtcggcgag
      301 tagtgagaac ccccagtggt tctcggggcc aaccgtgaag ccgtggctgg cgccgaaatc
      361 gcagtagtcc tggtacgccc gcgtgatcgt gtcgagctgc tccgaaccga agggcacctg
      421 tgtgcccccc gtatcgatgc ggaccgtctc ggcgccgagg atggcggctg ctcgaaggtg
      481 cgcctcggcc agctcgcggt tgcgcttccg ggcgtcggga tcgtcctccc agatgtggca
      541 gccgtcggcg tggtagttgg ccacggtcat ctcacgctcg tccagtgtcc gccgcagctt
      601 gcggaggaag ccctcttcca cactgcccac caggccattc cacaggtcgg ccgcatggat
      661 cccgtagcgg tactgcacgg tctcgagata tccaaacacg tcgatgcgcc cctcggccag
      721 caggccgtgg aagctgtacc cggcaatggt gatccgcatg gcgtcctccg ttgatgtccc
      781 agggaatggg gggctgccag ggccacagcc cggcgacctg ggtcgagatg gcgttcgccc
      841 ggagacgccg gtcgccaagc ggaaagggcg ccgcccgggc tgccctgagg ggcgctcccc
      901 ctactctccg cgcttccacg cccgcacgta ctcgatgctg tacgtggacg gcaggtcttc
      961 gtctttcggg agcccgaacc agtcgggcat ggtctcgctg tcgaagttca gcgtcagtgg
     1021 ctggtgccag tgcgtgttcg ggccccaacg gaccagcacg ccatcgaggt agaaccgcag
     1081 ctcccgctca tcccactcga gcccatacac atggtagtca tcgaccaggt cccatggcgt
     1141 ttcgtaggcg ccgccgatgg cgatgtgttg cctctcggcg ggcgttctca tgacgtgaac
     1201 ggtcatgttg agctttcgct cgtagcccgg ggcgcggccg ccgatctcgt agacgtcgat
     1261 ctcggtccac cagtcatcgg ttgagtcgta gaaccagaac gagctggagc ccgctgactt
     1321 cattggcttt gccttgacct cgaagtagcc gtagcggacc gtttgcttgc tcttgacggc
     1381 cgcgctcgtg taggtgtgat agccctcctc cttcgacgct tcggccactt cctgcttccg
     1441 catcgccagg tggagcttcc catcgctcac ggtcacgttg ccggcataga agaaggccgg
     1501 ctgccggccc ttccagcccg ggttgttggg ccaccacttg gtcgcatcga gtgtgtcgcc
     1561 ctcgaactcg tcggtgagcg gctcataggg cacccatcgg ccctcgttgg cctggtcgga
     1621 gaggggaaga tcgtcgcgga ctcgctgcac cccaccgccg ctcagatcgg gggtgaaggc
     1681 ctcccaggcg gcggcccact tgccgacgtc ttcggcctgt ggctcgattc ccgccgcgcc
     1741 gaagtcatcg cgcgccagcg ggctgacacg tgcggcgtag tcgacggggt cggacgggat
     1801 ggccacggca ccgacggcca ggccggcggc cacatcgaat gggccggcgt agcgccggga
     1861 cagcgaagac gcgcccgggc cgatgagcca gtagcgcagc tcgcacccgc gcggcccgct
     1921 gagtacgacg ttgcccgccg cgtcgcggct gatctccggg ctcggcgtcc cctgccccgc
     1981 cagcggtact accgaagccg ccagcacggt tcccgcgagc atgcatcggt ggatcgacat
     2041 gggtgtcact ctccctattg ctcttcgtgc ccgcgggcct cccggtccgc gccgttgacg
     2101 tgggttctgc tatggcgggc gaggacgttc gccggcacag gaccataggc cctactccag
     2161 cgccacctcg atgctcccct cgcccgtcca c
//
LOCUS       DAOSPL010000854         2189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_49981_length_2189_cov_3.392221, whole genome shotgun sequence.
ACCESSION   DAOSPL010000854 DAOSPL010000000
VERSION     DAOSPL010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49981_length_2189_cov_3.392221"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..>2189)
                     /locus_tag="PLQ54_14470"
     CDS             complement(99..>2189)
                     /locus_tag="PLQ54_14470"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOS94522.1"
                     /translation="NEIVPRMTHLLFALERHTGLKSILWVYGVGDHGGGPTRRHLRAA
                     QDMAQWPIWPRVKLTTTDAYFTDVEREIAQRDIQLPVHADELNFVFEGCYTSVSRIKF
                     ANRQGENDLVDTEVVALAGHRLAGLPYPSQTLTECWRRSMFIQFHDILPGSGVLETRE
                     HAMGLFQENLANTGMVRSRALRAIAQRVNTRAVAPARSPEGADQGLGAGVGNGAWWGS
                     VSAVGAGACGGDPFVVFNPCAFPRDELVTLKVWNRELPDGEVTVRDAQGHTTPGQVID
                     RGHYWGHQFTTIAFPARDLPAMGYRAYVVDRQRPPQGERACYVTEDGRAIYGLGYVHA
                     QMLGSVTMGNELLEMVVSPSHGGVISLIDKASGTEFVPPGEVFGCIDREQEAPHGMTA
                     WQLGAIIERTRPLAFSVMTVVHQGPNLAAVRLSAKHGQSQYDLTIALATGQRRVDFEL
                     TVNWLERGDPSTGVPALRASFPVAIDEGQATFEIPFGTIERPTDGEESPALNWADLSG
                     SAWGIGDEPEEKLGITLLNNCKYGHQAHDDMLRLTLIRSSYDPDPLPELGLHRIGYAI
                     VPHTGPLDPGSAARAGYAFNHPCIAVSTTVHEGELPPEATGIETLTPNVLVAAVKKAE
                     DSGATIIRLYEIEGRNTDAQIRLAPELVGPTTTASLTDILEQPLPGDGASICGGVLTV
                     TVPAFGIATVRIGE"
BASE COUNT          331 a          719 c          766 g          373 t
ORIGIN      
        1 cgtggttccc cccatggcgc ccctccgcaa ccaggctgat gggccacggg agccccaagt
       61 tgccgccaat gtcgaatagc ggcggaccag acgtaggcct actcgccgat cctcaccgtc
      121 gcgattccga acgccggcac cgtcacggtg agcacaccgc cgcagatgct ggcgccatcc
      181 ccgggcagag gctgctcgag gatgtcggtc agcgaggcgg tggtagtcgg cccgacgagc
      241 tctggagcca ggcgaatctg tgcgtcggtg ttgcggcctt cgatctcata cagccggatg
      301 atcgtggccc cggagtcctc cgccttcttg accgccgcca caagcacatt aggagtgagt
      361 gtctcgatgc cggtggcttc gggtggaagc tctccctcgt gaaccgtggt gctgaccgcg
      421 atgcatgggt ggttgaacgc ataacccgcc cgggccgccg agccggggtc gagcgggccc
      481 gtatggggca cgatggcgta gccaatgcga tgcagcccga gctcgggcag cggatcggga
      541 tcgtagctcg agcggatgag tgtcagccgc agcatatcgt cgtgggcctg gtggccgtat
      601 ttgcagttgt tgagtaatgt gatcccgagc ttctcttcgg gctcgtcgcc gatgccccac
      661 gccgatccgc tgaggtcggc ccagttgagc gcgggggact cctcgccgtc ggtgggccgc
      721 tcgatagtcc cgaaggggat ctcgaaggtg gcctggccct cgtcgatggc gacggggaag
      781 gacgcgcgga gcgccgggac tcccgtcgac ggatcgccgc gctcgagcca gttcacggtg
      841 agctcgaagt ccacgcgccg ctgcccggtg gcgagggcga tcgtcaggtc gtattgcgac
      901 tggccgtgct tcgccgagag ccgcacggcc gccaggttcg gcccctggtg caccactgtc
      961 atgacggaga acgccagcgg ccgcgtgcgc tcgatgatcg ctccgagctg ccaggctgtc
     1021 atgccgtggg gtgcctcttg ctctcggtcg atgcacccga atacctctcc tggcggcacg
     1081 aactcggtgc cgcttgcctt gtcgatcagg ctgataacgc cgccatggct ggggctgacg
     1141 accatctcca ggagctcatt ccccatggtg accgaaccca gcatctgggc gtgaacgtag
     1201 cccaagccat agatggcgcg gccgtcctcc gtcacatagc aggccctctc gccctggggc
     1261 ggtcgttggc ggtcaacgac gtacgcgcgg taccccatgg cgggcaaatc ccgagccggg
     1321 aaggcgatgg tggtgaactg gtggccccag tagtggccgc gatcgatcac ctggccgggg
     1381 gtggtgtggc cctgcgcgtc gcggaccgtg acctccccgt cgggcagttc ccggttccag
     1441 accttgagtg tgaccagctc gtcgcgcggg aatgcgcacg ggttgaacac cacgaaggga
     1501 tcgcccccgc aggcgccggc gccgacggcg gagaccgatc cccaccatgc cccgtttccc
     1561 acgcctgcgc cgaggccctg gtcggcgccc tccgggctcc tcgcgggagc cacggcccgg
     1621 gtgttgaccc gctgtgcgat ggcccggagc gcccgcgagc ggaccatgcc cgtgttcgcc
     1681 aggttctcct ggaaaagccc catggcgtgc tcgcgggtct cgagcacgcc cgagcccggg
     1741 aggatgtcgt ggaactggat gaacatggac cgccgccagc actcggttag cgtctggctc
     1801 gggtacggca gcccggccaa ccggtgaccg gcgagcgcca ccacttcggt gtcgaccaag
     1861 tcgttctcgc cctgacggtt ggcgaacttg atgcggctga ccgacgtata gcagccctcg
     1921 aacacgaagt tgagttcgtc ggcgtgcacc gggagttgga tgtcgcgctg tgcgatctcg
     1981 cgctcgacgt cggtgaagta ggcatcggtg gtggtgagct tcacccgggg ccagatgggc
     2041 cactgggcca tgtcttgggc ggcgcgcagg tgacgccggg tggggccgcc gccgtggtcg
     2101 cccacgccat agacccagag gatggacttg aggcccgtgt ggcgctcgag ggcaaacagc
     2161 agatgggtca tccgcggcac gatctcgtt
//
LOCUS       DAOSPL010000855         2187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_50031_length_2187_cov_3.949812, whole genome shotgun sequence.
ACCESSION   DAOSPL010000855 DAOSPL010000000
VERSION     DAOSPL010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50031_length_2187_cov_3.949812"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..281)
                     /locus_tag="PLQ54_14475"
     CDS             complement(66..281)
                     /locus_tag="PLQ54_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94523.1"
                     /translation="MACVNWRAVDLGARVLMGVLLVVGAVFFVQDTGGRGATLCFVIV
                     IVLSLLSRYAGTRSNPVGSPPPPQQSR"
     gene            complement(352..1089)
                     /locus_tag="PLQ54_14480"
     CDS             complement(352..1089)
                     /locus_tag="PLQ54_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94524.1"
                     /translation="MTPGTTHPLVAAYLEELDGLLAGIEPRERADVMLGVREHLDGAL
                     GATGGTDQEVREALAELGPPQLVANEAYAGRQPATAATPGPTPVMARSWVPVVVAVLT
                     AVALLMAVFIVSSMGSVVSSSSSSSSDANGVITQEQTSEVLAGSPLIGSLAALMTTLP
                     LWATVAVLVGVSPLWVRREKVIAILLIPAAALLLGLLPQVGWWLIGANGVYGGAWAAL
                     ILVLLGGGAILTRSTRKAAARVHHPAA"
     gene            complement(1086..1433)
                     /locus_tag="PLQ54_14485"
     CDS             complement(1086..1433)
                     /locus_tag="PLQ54_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOS94525.1"
                     /translation="MVPAEETILTNLRRGALEFCVLAMLTDEELYGLDIARRLTVDGL
                     LMSSEGTLYPLLTRLRTAGLVTTTWQQSVEGPPRRYYALTPDGRDSLAAFTRTWPAFR
                     DAVDHALDRRDLP"
     gene            complement(1570..2061)
                     /locus_tag="PLQ54_14490"
     CDS             complement(1570..2061)
                     /locus_tag="PLQ54_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94526.1"
                     /translation="MGTGVVFDVRSVGYVALCTGIAALLLVVSVGAFVDPALEGSRDP
                     DWAFWLWGSCLVAVLCRAPFVGLLIRDERVIRRSWVRTQSWSVSEVSGVETAGYSGNL
                     NRGSQSGRFLMLVLRMRDGSVVEVPEVSGERRKMNDRLHRVAHALRLPAPSEPGRHRI
                     AEP"
BASE COUNT          409 a          717 c          747 g          314 t
ORIGIN      
        1 ccagcgatac ctccgcgccc tggatcggct gtaggaaacg tccgaaactg ccgcggagcg
       61 cgggtctacc tgctctgctg aggaggaggg ggagaaccca ccgggttact cctggtcccc
      121 gcgtagcggc tcaagaggct caagacgatg acaatcacga agcagagcgt cgccccgcgg
      181 ccacccgtgt cttggacgaa gaacacggcg ccgacgacga gcaggacccc catcaagacg
      241 cgcgctccca agtccacggc ccgccagttc acgcaagcca tgctcgcacg gccacgtggc
      301 ggcctgcgcc cctttcacgg tgatgccgca gtcagagtgc tggaggtcga ttcaggcagc
      361 tgggtggtgc acgcgtgcgg cggcctttcg ggtgcttcgg gtcaggattg ctccgcctcc
      421 caggagaacc aggatcagtg cggcccaggc ccctccgtag acgccgttgg cgccgatcag
      481 ccaccagccg acctgcggca gaaggcccag cagcagggcc gcggcgggga tcagcaggat
      541 cgcgatgacc ttctcccggc gtacccacaa gggcgagacc ccaacgagga cggcgacggt
      601 ggcccacagg ggaagcgtgg tcatcagggc ggcgagcgag ccaatcagcg gcgagccggc
      661 caacacctcg ctggtctgct cttgagtgat gactccgttg gcatcgctcg acgacgacga
      721 ggagctgctc acgacgcttc ccatcgagct gacgataaag acggccatga gcagggccac
      781 cgcggtcagg acggccacca cgaccggcac ccacgagcga gccatgaccg gagtcggacc
      841 gggcgtggct gctgtggcgg gttggcggcc ggcgtaggcc tcgttggcga ccagttgggg
      901 cgggccgagc tcggccaggg cctcgcggac ctcctggtcg gtgccgcccg tggctcccag
      961 ggcgccgtcg aggtgttcgc gtacaccgag catgacgtcg gcacgctcgc gcggttcgat
     1021 gccagcaagg agcccgtcga gctcttccag gtatgccgca acgagtgggt gggtggtgcc
     1081 cggggtcatg gcaggtccct tcggtcgagt gcgtggtcga ccgcgtctcg gaacgcgggc
     1141 caggtgcgag tgaaagcggc caaggagtct cggccgtctg gggtgagggc gtagtaccgg
     1201 cgtggggggc cctccaccga ctgctgccag gtcgtcgtga ccagtccggc cgttcgcagg
     1261 cgcgtcagca gcgggtagag cgttccctcg ctggacatca gcagcccgtc gacagtgagg
     1321 cgccgggcga tgtccaagcc gtagagctcc tcgtcagtga gcatggcgag gacgcagaac
     1381 tccaaggcgc ctcggcgcag gttcgtgagg atcgtctcct cagccggaac catgcgttgc
     1441 aacctagtgg atggttgcga cacaagcaac cgaaatgggg gtgcaaaccg tgggccccag
     1501 tcatccgcta ttgccgcgga tggtgaacgt ggcgaccatg ccgagatgag cgagtccagg
     1561 cacccagccc tacggctctg caatgcggtg acgacctggt tccgacgggg ccggaaggcg
     1621 tagcgcgtgt gcgacccggt ggagtcggtc gttcatcttg cgacgctccc ccgagacctc
     1681 cggaacttcg acaacggacc cgtcgcgcat gcgcagcacg agcatgagga accggcccga
     1741 ttgtgagccc cggttgaggt tgcccgaata gcccgccgtt tcgacgccgg atacctcact
     1801 caccgaccag gactgtgtgc gcacccacga acgacgaatc acacgttcat cacgaatcag
     1861 cagaccaacg aagggtgcac ggcacagcac ggccacgagg caggagcccc acagccagaa
     1921 cgcccagtca gggtcgcgtg agccctccag cgctgggtcc acgaaagcac ccaccgacac
     1981 gacaaggagc agtgccgcga tgcccgtgca cagagcgacg tagccgaccg accggacgtc
     2041 gaatacaact cccgtgccca tgacgcaagg gtgtcacgtc cgcagtcctc gcccgaagcg
     2101 cacgctcatc agcgtgaggc gattaccgga tctgccgctg agtgcgaagt agtcgtagct
     2161 aacggctcgg tggaagattc gagcgcg
//
LOCUS       DAOSPL010000856         2187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_50036_length_2187_cov_3.125235, whole genome shotgun sequence.
ACCESSION   DAOSPL010000856 DAOSPL010000000
VERSION     DAOSPL010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50036_length_2187_cov_3.125235"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLQ54_14495"
     CDS             complement(<1..214)
                     /locus_tag="PLQ54_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOS94527.1"
                     /translation="MVEKVRAVAQRCIEEGKGLVPLIQAVQAECEYLPPEALRTIADT
                     TGVPLSQVYSVATFYHSFRLKPKGKHT"
     gene            728..1570
                     /gene="rpsB"
                     /locus_tag="PLQ54_14500"
     CDS             728..1570
                     /gene="rpsB"
                     /locus_tag="PLQ54_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOS94528.1"
                     /translation="MALVSMKELLEAGVHFGHQTRRWNPKMKPYIYGGRNGIYIIDLH
                     QTLKKIDEAYDFVRRVALEGKPVLFIGTKKQGQETIQRAAERCGQYYVNHRWLGGMLT
                     NFVTIRGRINRLKELTQMEQDGSLELRPKKEAARLMLEREKLAKNLGGIMDMPSLPGA
                     VFVVDLKKEHIALRECRRLEIPTVGIVDTNCDPDEVDYIIPANDDAIRAIRLLTNLMA
                     DAVIEGQREREARAADEAGEESEGAAADSDEAYDYPHAPEEAGAEAEYAYEPEDEEED
                     ASAV"
     gene            1702..>2187
                     /gene="tsf"
                     /locus_tag="PLQ54_14505"
     CDS             1702..>2187
                     /gene="tsf"
                     /locus_tag="PLQ54_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOS94529.1"
                     /translation="MAITASQVKELRERTGAGMMDCKQALTEADGDMDAAIEWLRKKG
                     LSAAAKRATRQASEGAVFAYVHTNQKIGVLVELNCETDFVARTEGFRNLGAELALQVA
                     AMAPEYVSQQDIPAERIERETQILTELTRNEGKPEQAIPKIVEGRVKKLKVEQKECEV
                     DI"
BASE COUNT          398 a          679 c          757 g          353 t
ORIGIN      
        1 acgtgtgctt ccctttgggc ttgaggcgga acgaatggta gaaggtcgcc acgctgtaga
       61 cctggctcag gggcacgccg gtggtgtcgg cgattgtgcg aagggcctcc ggcggcaggt
      121 actcgcactc ggcctgaacg gcctggatca ggggcacgag gcccttgccc tcctcgatgc
      181 agcgttgcgc aaccgcgcga accttctcca ccatcgatcc ggcctttccc gtgaccagcc
      241 cgcccgggcg caggcatgcg tgcacggcca gtgactgtgc tctggatgcg tggcgtaaac
      301 ccagcgggaa cccggacttc cgcgcgcggg aagcccggga gagacaacgg tagtcagcca
      361 aacgcgttga cgagcccggg ggcattccgg cgccccacgc cccttgcact gttcgtctct
      421 agaataccct gcgggcaggt tccggggcaa ccacggcgat gggccgggag tcggccttgc
      481 cccgcgcgat gcggccgtgg tagactctgg gggccgcttc gcggggccgt gcctcgtcgg
      541 agcctggtat gtggtcgcgt ttcaccaccg gatgtccccc atggctttcc ctgggctccg
      601 gcttgctgcc ggtgcgtccc cggtgtgggg actgcgtccg cggtggcgac ccatcagatc
      661 tcacgtcgcc cctgtcgacg tggttgcggc aaccgatgct ggaatcggtt gcaggaggtg
      721 cgtctgtttg gccctcgttt cgatgaagga actcctggag gccggtgtgc acttcggcca
      781 ccagacccgc cgctggaatc ccaagatgaa accctacatc tacggcgggc gcaacgggat
      841 ctacattatc gacctgcacc agaccctcaa gaagatcgac gaagcctacg acttcgtgcg
      901 ccgcgtggcc ctcgagggca agcccgtcct cttcatcggt accaagaagc aggggcagga
      961 gaccatccag cgcgcggcgg aacgttgtgg gcagtattac gtgaatcatc gctggctggg
     1021 tggcatgctg accaacttcg tgaccatccg tggccgcatc aaccgcctca aggagctaac
     1081 ccagatggag caagatggct ccctggagct ccgccccaag aaagaggcgg cccgcttgat
     1141 gctcgagcgc gagaagctgg ccaagaacct cgggggcatt atggacatgc ccagcttgcc
     1201 gggggccgtg tttgtggtgg atctgaagaa ggagcacatc gcgctgcgcg agtgccgccg
     1261 gctggagatc ccgacggtgg gcatcgtaga cacgaactgc gatcccgacg aagtggacta
     1321 catcatcccg gccaacgatg atgcgattcg cgcgattcgc ctgctgacga acctgatggc
     1381 cgatgcggtc atcgagggcc agcgggagcg cgaggcacgc gcggctgacg aggccggcga
     1441 ggagtccgag ggcgccgccg cggattccga cgaagcctat gactacccgc acgcgcccga
     1501 agaagcgggt gccgaggccg agtatgccta tgagcccgag gacgaggaag aagacgcctc
     1561 ggccgtctag gacacgttcg cctgctctcg ggcccgcgat gaggacgcgc actggcgccg
     1621 tgtggtgggc gtgggcccgc ggggtgggat catgggttcc gcgggtcggc gggcccactt
     1681 gtgcaaggag ctgactcccc gatggcgatt accgcttccc aggtcaagga actccgcgag
     1741 cgcactggcg cggggatgat ggactgcaaa caggcactga ccgaggccga tggcgacatg
     1801 gacgcggcca tcgagtggtt gcggaagaag ggcctctcgg ctgccgccaa gcgtgcgacg
     1861 cgccaggcca gcgaaggtgc ggtgtttgca tacgttcaca cgaaccagaa gatcggggtc
     1921 ctggtcgagc tcaactgcga gaccgacttc gtcgcgcgca ccgagggctt caggaacctg
     1981 ggtgccgagt tggcccttca ggtggcggcc atggcgcccg agtacgtgag ccagcaggac
     2041 atcccggccg agcgcatcga gcgggaaacc cagattctga cggaactcac gcgcaatgag
     2101 ggcaagcccg agcaggcgat ccccaagatc gttgagggcc gcgtcaagaa gctcaaggtt
     2161 gagcagaagg agtgcgaggt cgacatc
//
LOCUS       DAOSPL010000857         2186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_50060_length_2186_cov_3.565462, whole genome shotgun sequence.
ACCESSION   DAOSPL010000857 DAOSPL010000000
VERSION     DAOSPL010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50060_length_2186_cov_3.565462"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="PLQ54_14510"
     CDS             <1..1334
                     /locus_tag="PLQ54_14510"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOS94530.1"
                     /translation="FAQLMQWARENRARLQSPEPFGGDPSAREVYGYAYPRLDRPLYF
                     ARNPWISEAEVVLPECRTDGARQLVMHYPARCVLAVARSGEALPRVALGPYETAVLEV
                     LPVPARGVAPAVVPGLGVEWRPVASPAFERVTFEDDRPPLGPSWTSPDGDSPEALVLS
                     GSGELRTSRPAELCLLAEGPAGSAMPLCDIVVDGQVVAPEVTGSRGTFYAAGATPQED
                     WQWFLAPLPAGRHRVSYRLTVPDDECRCAVFVRGERRAGSGGAEPPVGPSFPVKPAGL
                     RPWSVTLVPLANAKEAGATSSRMKRRTTDVDGVYLDALDWTEATAGWGQVHRNASVMG
                     TPMVIGNRVHRRGIGTHANSRITYQIDGAYTAFVATVGCDQEVSGNSVVFAVEGDGAE
                     LYRSPVMRVRSTPIPIRVDIRGVRRLTLVVTDGGDGIGADHADWAEAVLLR"
     gene            complement(1366..>2186)
                     /locus_tag="PLQ54_14515"
     CDS             complement(1366..>2186)
                     /locus_tag="PLQ54_14515"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOS94531.1"
                     /translation="IVVGDFLYSELIADPFSFDGNPVIRTLATHKDILELARRHGREV
                     WFDVHVTSERPESVACIDPLPTLASALRQLCPGAQFKLAVFELNANQHDLGRGLANAR
                     AINQLERMGDLVRIVCSANCLQPDGQNDNGWNQGLLFLNPSRVWPQAAYFVTQMLSEH
                     YLSVCVGADVAGQADCLDVTAKTSSDGRLVQLQMVNWADEPTRLTIRLEPHGSLARPA
                     HRVELCGGTNQTNTAAEPDAVVPVAGDIDLVSTGAATEYVLPPRSFTVLRARAE"
BASE COUNT          312 a          731 c          766 g          377 t
ORIGIN      
        1 tctttgccca gctgatgcag tgggcacggg agaaccgcgc cagactccag agccctgagc
       61 cattcggcgg ggatccctcg gcgagggagg tttacggata cgcctacccg cggctcgacc
      121 ggcctctcta cttcgcgcgc aacccgtgga tctcggaggc cgaggtggtc ttgcccgagt
      181 gcaggaccga tggcgcccgg cagttggtga tgcactaccc ggctcgctgc gtgctcgccg
      241 ttgcccgcag cggcgaggcc cttccccgtg tggccctggg cccctatgag acggcggtac
      301 tcgaggtgct tccggtccct gctcgcggtg tcgcaccggc tgtagtgcct ggtctcgggg
      361 tggagtggcg gccggtggcg agtccggctt tcgagcgggt cacgttcgag gatgaccgtc
      421 ctccccttgg cccatcgtgg acctcgcccg atggcgattc cccggaggcg ctggtgctct
      481 cgggctctgg cgagttgcgg acctcccggc cggcggagct ctgtctgctg gccgaagggc
      541 ctgccggaag cgcaatgccg ctgtgcgaca tcgttgttga cggccaggtg gttgcccccg
      601 aggtcaccgg ctcccgcggt accttctatg ctgcgggcgc cacaccccag gaggactggc
      661 agtggttcct cgcccccctg ccggcgggac ggcatcgtgt gtcctaccgg ctgaccgtcc
      721 cggatgacga gtgccgctgc gccgtcttcg tgcgcggcga gcggcgggcc ggctccggtg
      781 gcgcggagcc gccagtcggc cccagcttcc ccgtgaagcc tgccgggttg cgcccgtggt
      841 cagtgacgct cgtgccgctg gcgaatgcca aggaggcggg cgcgaccagt tcccgcatga
      901 agcggcgcac gaccgatgtg gacggggtgt acctggatgc gctcgattgg accgaagcca
      961 cggccggttg gggccaggtc caccggaacg ccagcgtcat ggggaccccc atggtcatcg
     1021 gcaaccgggt gcaccgtcgc ggcatcggca cccacgcgaa ctccaggatc acctatcaga
     1081 tcgatggtgc ctacacggcc ttcgtggcca cggtcgggtg cgaccaggag gtctcgggca
     1141 actcggtggt ctttgccgtc gagggagacg gtgctgagct gtatcgcagc ccggtcatgc
     1201 gcgttcgctc gacgccgatc ccgatccggg tggacatccg gggcgtccgc cggctcacac
     1261 tggttgtcac cgatggcggc gacggtatcg gtgcggacca cgccgattgg gccgaggccg
     1321 tgctactgcg ctgaggccgc cgcgccagcg cgcccccgcg cccctctact ccgctcgcgc
     1381 gcgcagcacc gtgaaggagc gcggtggaag aacgtactcg gtcgccgcgc cggtggatac
     1441 gagatcgatg tcccctgcca cagggaccac ggcgtccggt tcggcggcgg tgttggtttg
     1501 gttagtcccc ccgcatagtt cgacgcggtg tgccggcctg gccagcgagc cgtggggctc
     1561 caggcgtatg gtgagacgcg tgggctcatc ggcccagttg accatctgca gctggaccaa
     1621 ccggccgtcg gagctggtct tggcggttac atcaaggcag tcggcctgcc cggcgacatc
     1681 ggcccccaca cacaccgaca gatagtgctc cgacagcatc tgcgtgacga agtaggcggc
     1741 ctgaggccag acgcgcgacg ggttcaggaa cagcaggccc tggttccaac cgttgtcatt
     1801 ctgcccgtcg ggctgcaggc agttggccga gcagacgatg cggaccaggt cgcccatccg
     1861 ctcgagctgg ttgatggccc gtgcgttggc caggccccgg cccagatcgt gctggtttgc
     1921 gttgagctcg aacacggcca acttgaactg ggctccagga cacagctgtc tcagggcact
     1981 tgccagcgtt ggcagtggat caatgcacgc cacagactcc gggcgctcgc tcgttacgtg
     2041 cacgtcgaac cacacctcgc ggccatggcg ccgcgccagt tcgagaatgt ccttgtgggt
     2101 tgccagcgtc cggataaccg ggttcccatc gaagctgaag gggtccgcga tcagctcgga
     2161 gtagaggaag tcgccgacca cgatcg
//
LOCUS       DAOSPL010000858         2185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_50084_length_2185_cov_4.019718, whole genome shotgun sequence.
ACCESSION   DAOSPL010000858 DAOSPL010000000
VERSION     DAOSPL010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50084_length_2185_cov_4.019718"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="PLQ54_14520"
     CDS             complement(<1..1333)
                     /locus_tag="PLQ54_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94532.1"
                     /translation="MAPLLALAMAGAAVPALAQVGRVYCNVTEVKATQLSNAVRIDLR
                     CDGQLEVAADLTDYVEVDPRQGGVTAAKPVRNIVLRLTNAKSRVGAFIDVGRYPVSHV
                     EMAPLPDSPDGVGVACTISLYPPGRTNGLRMAPYYLDPHDFIEAGLGFRPGEVRWLVQ
                     EADDRRGLVFIVTSDRFAIDADSSPEEEVPYTLDIGYADGLVEVRAVRAPIGALAETI
                     GARTGLRCTVAGDPGRRVSLYVPPTRPERVLSELARSQGLTLMKDPAGPMQLQALAPR
                     DPATYDGTETRLLRLNHLTAEEALGLLPVCLLRYVRPSPEQNALVASGSTELLDHLQA
                     NLQAVDLPAPIIEVVVEVLAHFSDRGSLAHLAIDGQAGRWVGGASGEGLEAFCTPVGA
                     LRPELEAHIRDLRLAGELDVVTRQRLSITNGSKGHVFVGDQPTIVTSYYDYF"
     gene            complement(1363..>2185)
                     /locus_tag="PLQ54_14525"
     CDS             complement(1363..>2185)
                     /locus_tag="PLQ54_14525"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HOS94533.1"
                     /translation="LGDLLRLELGVEILEKFRGDVKQFDVPAAQIMIEVLMVEFGETN
                     SDEFYAKLGWSRNGRGVTTDSARGAATFHVLGDLPVGFTAELRAMEAAGRAHVRANPR
                     IATVSGREASVFIGERRFLAKPVEMSGGDDGNDDNRRQINFINAGVNLSITPWTGGEG
                     EIQAQIQTEVSTMSAPDAVTGLPQKQTRSASTDIRMLDGETVLIGGLVQDEMIETKSK
                     IPVLGDIPLIGKLFRSKNTRRFQTELVVFVTARILPGSNGNGQPHAAKEARATAP"
BASE COUNT          344 a          715 c          758 g          368 t
ORIGIN      
        1 cgaagtagtc atagtagctg gtcacaatcg tcggctggtc accgacgaac acgtggccct
       61 tgctaccatt ggtgatgctg agacgctgcc gggtcacgac gtcgagctcg ccggcgaggc
      121 gcaggtcgcg gatgtgcgct tcgagttcgg gtcgcagagc tccgacgggc gtacagaatg
      181 cctcgaggcc ctccccgctc gccccaccaa cccagcggcc cgcctggccg tcgatggcga
      241 ggtgggccag tgagccacga tcgctgaagt gggccaggac ctcgacgacg acctcgatga
      301 tcggggcggg caggtcgacg gcctgcagat tcgcctgcag gtggtcgagc agctcggtgg
      361 agcccgacgc gacgagggcg ttctgctcgg ggctgggtcg cacatagcgc agaaggcaca
      421 ccggcaggag cccgagtgcc tcctcggccg tcaggtggtt cagccggagc aagcgtgtct
      481 ccgtaccgtc ataggtggcc gggtcgcggg gagcgagggc ctgcagctgc atgggaccgg
      541 cggggtcttt catcagcgtc agcccctggg agcgggcgag ttcactcaac acccgctccg
      601 gccgagtggg cggcacgtag agtgacacgc gccggcccgg gtcgccggcc acggtgcaac
      661 ggagcccggt gcgcgctccg atggtttccg ccaatgcgcc gatcggcgcg cgcacggcac
      721 gcacttccac cagcccgtcg gcatagccga tgtcgagcgt ataggggacc tcttcctcag
      781 gggaggagtc ggcatcgatg gcgaagcgat ccgaggtcac gatgaacacg aggccgcgcc
      841 ggtcgtcggc ctcctgaaca agccatcgga cctcgccggg cctgaagccc aggccggcct
      901 cgatgaagtc gtggggatcg aggtagtacg gagccatacg gaggccgttg gtgcggcccg
      961 gggggtagag cgagatcgtg caggccacac cgacgccatc ggggctgtcg ggcagggggg
     1021 ccatctcgac gtggctcacg gggtagcgac cgacatcgat gaacgcgccc accctggact
     1081 tggcgttcgt cagccgcaac acgatgttcc ggaccggctt ggcggcggtg acgccgccct
     1141 gccgcggatc cacctcgacg tagtccgtga ggtccgcggc gacctcgagc tgcccgtcgc
     1201 accggagatc gatgcgaacc gcgttcgaga gctgcgtggc cttcacctcg gtcacgttgc
     1261 agtacacgcg gccgacctgc gccagggcag ggacggccgc gcctgccatc gccagggcaa
     1321 gcaggggggc catgccgaat cgaggggcag cgtgatgggg cgtcatggtg cggtcgcgcg
     1381 ggcctccttg gcggcgtgtg gttggccatt cccgttgctt cccggcagga tcctggcggt
     1441 tacgaacacc accagctcgg tctggaatcg ccgtgtgttc ttgctacgga agagtttgcc
     1501 gatcagcggg atgtcgccga ggaccggtat cttggacttg gtctcgatca tctcatcctg
     1561 gaccagcccg ccaatgagca ccgtctcgcc gtcgagcatc cggatgtccg tgctggcgga
     1621 tcgagtctgt ttctggggta acccggttac ggcatcgggt gcgctcatgg ttgagacctc
     1681 ggtctggatc tgagcctgga tctcgccctc tccgccggtc cacggcgtga tgctgaggtt
     1741 cactccggcg ttgatgaagt tgatctgccg ccggttgtca tcgttgccat catctccgcc
     1801 cgacatctcg acgggcttgg ccaggaaccg cctttcaccg atgaagacac tggcctcgcg
     1861 cccgctgacg gtggcgatcc gcgggttggc gcgaacgtgg gctctgccgg cggcctccat
     1921 ggcccggagc tccgccgtga agcccaccgg cagatcgccc agcacgtgga acgtggccgc
     1981 cccccgagcc gagtcggtgg tgactccccg gccgttccgc gaccaaccga gcttggcgta
     2041 gaactcgtcg ctgttcgtct cgccaaactc gaccatcagg acctcgatca tgatctgagc
     2101 ggccggcaca tcgaactgct tcacgtcgcc gcggaacttc tcgaggatct cgaccccaag
     2161 ctctagccgg agcaggtcgc cgagg
//
LOCUS       DAOSPL010000859         2184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_50128_length_2184_cov_3.428370, whole genome shotgun sequence.
ACCESSION   DAOSPL010000859 DAOSPL010000000
VERSION     DAOSPL010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50128_length_2184_cov_3.428370"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /locus_tag="PLQ54_14530"
     CDS             complement(<1..1408)
                     /locus_tag="PLQ54_14530"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOS94534.1"
                     /translation="MIRTLVVTRSGRDPEAESARAAFAAEGHPDVRVRFESVFRLEGN
                     CDLERIGRLFANPAAGETLVEDQGLERAGDECVVEVAYRPAVVDPETESALEAARLMG
                     VPGLEWLRAGRRYVVTGVDRAEAERVITKHLFNEVVQVIVRPGDYQQSLRPTGSPEPV
                     QTYDLMGLSDAELSELSQRMGWYAPLGQLQALRAHQAERGRPWTDVEIEMVVQSWGDH
                     CYHTTWKGLGLLKGLMAATREINHPLCLSIFTDNAGAIRFYGGYAVFIKGETHNHPSA
                     ISTKGGVETKHGGVLRDIIFMGRGGYPIGSSTIMGTRRPSEPESEVPAGALHPRTIVL
                     ESISGTASYCNPMGVPMMYALYRAHPGYAKCMALGQCIGIAPEDTIEKLEPQPGDVLL
                     LIGGATGRDGVHGAVASSAQLTAEMVEKEGATVQIGHPIVERKFTNLVPALRDKGWIR
                     TCTDLGAGGISCCVGEIAA"
     gene            complement(1553..>2184)
                     /locus_tag="PLQ54_14535"
     CDS             complement(1553..>2184)
                     /locus_tag="PLQ54_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94535.1"
                     /translation="LFRLTVHQGALFSAAPAEATVRVVPTGTIHVELTWQTPNDPNPA
                     DSGSDAGADMDLHLTHARAPARPDNTDMDGDGKPDPYFDTLYDCYWFQPLPDWGPAGT
                     AGNPSLDRDDTDGAGPEVISIQSPEANAVYRVAVHYWRDHGYGLSLATVRVRLNGTLA
                     FERTGVEMADRDLWCVADISATAGTVTALIAPAGGGDWIVHNYSHPDFQ"
BASE COUNT          332 a          715 c          769 g          368 t
ORIGIN      
        1 gggccgcgat ctcaccgacg cagcacgaga tgccccccgc gcccaggtcg gtgcacgtgc
       61 ggatccagcc cttgtcccga agcgcaggca ctaagttagt gaatttacgt tcgacaatcg
      121 ggtgcccaat ctggaccgtg gcaccttcct tctcgaccat ctccgccgtg agctgggccg
      181 aagaggcgac cgccccatgc actccgtctc ggcccgttgc gccgccgatc agcagcagta
      241 cgtcgccggg ctgaggttcg agtttctcga tagtgtcctc cggtgcgatc ccgatgcact
      301 gcccgagggc catgcacttg gcgtagcccg gatgggcccg gtacagggcg tacatcatgg
      361 gcacgcccat ggggttgcag taggaggccg tgccgctgat ggactcgagg acgatggtac
      421 gggggtgcag cgctcccgcg ggaacctcag actcgggctc cgatggcctc cgagtgccca
      481 tgatggtcga cgagccgatg gggtacccgc cgcggcccat gaagatgatg tcgcgcagca
      541 cgccgccgtg cttggtctca acgccgccct tggtggagat ggctgagggg tggttgtgag
      601 tttccccctt gatgaagacg gcgtatccgc cgtagaagcg gatggcgccg gcgttgtcgg
      661 tgaagatcga gagacacagc ggatgattga tctctcgcgt ggcggccatg agccccttca
      721 gcaggcccag gcccttccac gtggtgtggt agcagtggtc accccaggac tggacgacca
      781 tctcaatctc gacatcggtc cacggacggc cgcgctcggc ttggtgggcg cggagggcct
      841 gaagctgccc gagcggcgca taccatccca tgcgctggct gagctcggag agctcggcat
      901 ccgagagtcc catgaggtcg taggtctgta cgggctctgg ggagcccgtg ggacggaggc
      961 tctgctggta gtcgccgggc ctgacaatga cctggacgac ctcgttgaag aggtgtttcg
     1021 tgatgacccg ctcggcctcg gcgcggtcca caccagtcac gacgtaacgt cggcctgccc
     1081 ggagccattc gaggcccgga acgcccatca gccgtgcggc ctcgagggcc gactcggtct
     1141 ccgggtccac cacggccggg cgataggcga cctcgacgac acactcgtcg cccgcgcgct
     1201 cgagcccctg gtcctcaacc agcgtctcac ctgccgccgg gttggcgaag aggcggccga
     1261 tccgctccag gtcgcagttg ccctcgagcc ggaacacgga ctcgaatctg acgcggacat
     1321 ccgggtgccc ctcggcggcg aacgcggccc gagcgctctc agcctccgga tcgcggcccg
     1381 accgggtgac gactagggtg cggatcatgc tggcctcatc tcccctgccg ggcagtacaa
     1441 ccgacgccct gcgggggcca aaggctggcg agtggccgcg acgcgagcgg ccgtgagcaa
     1501 gcgggccagc cggcgcgaga ccgagcaccg cgcggctgcg cgatgtccgc cgctattgga
     1561 agtccgggtg cgagtagttg tggacgatcc agtcgccacc tccggccggg gcgatgaggg
     1621 ccgtcacggt gcctgcggtc gcgctgatgt cggccacgca ccacaggtcg cggtcggcca
     1681 tctcgacgcc ggtccgctcg aaggccagtg tgccgttgag ccgcactctg actgtggcca
     1741 gtgaaagacc gtagccgtgg tcgcgccagt aatgcacggc cactcggtat acggcgttgg
     1801 cttcggggga ctggatgctg atcacctccg gcccggcgcc gtcggtatcg tcgcggtcga
     1861 ggctgggatt gccggcggtc cctgctggcc cccagtccgg gagcggctgg aaccagtagc
     1921 agtcgtacag ggtgtcgaag tacgggtcgg gcttgccgtc gccgtccatg tcggtgttgt
     1981 caggccgggc tggcgcccgc gcgtgggtca ggtgcagatc catgtcggcg cctgcgtcag
     2041 agcctgagtc cgcagggttc gggtcgttgg gtgtctgcca ggtcaactcc acatggatgg
     2101 tgcccgtcgg gacgacgcgt accgttgcct ccgccggcgc ggcactgaac agcgccccct
     2161 ggtgtaccgt gaggcggaag aggt
//
LOCUS       DAOSPL010000860         2183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_50166_length_2183_cov_2.921992, whole genome shotgun sequence.
ACCESSION   DAOSPL010000860 DAOSPL010000000
VERSION     DAOSPL010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50166_length_2183_cov_2.921992"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1939)
                     /locus_tag="PLQ54_14540"
     CDS             complement(<1..1939)
                     /locus_tag="PLQ54_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94536.1"
                     /translation="MPTVPHAVCVAALIPVAWTAAEPLVLQNRALRLEFRSDPLALTA
                     VRNRLTGEVYTVEGDQFAIELGSGTIEQAYARRASLSADRTSVRARYEADGVRIDLSY
                     TLGPANHFVEKRLALTFTRDDTLRQVTVSRPAFAAPGLELVRYRYPQFGRVPGAEPCS
                     TFFGRTGRGSLFAGLAMPFDRSTGGGNAIELAYLPSVKVSAGQTVPCEAMYLGVCARS
                     EADTRPLPPPIRCSPSDPGAPVELREAEPGIVPLASESAAMVAMTESWLGPPRHGFVP
                     MACGWHSEMQQGPYASDEDVEADMRSLEFLADCGIDWFSESHPWGGETERMNALGPDD
                     HYTPGPRARRLLEHARDLGMGAVMWSSMTNTHHWSPLGRPFRADQPDWLLTPGPVSRA
                     ISIIASAKGNCVANRPFFEWLSRINREGFSTGLYRGWAMDGSFFGDGGWYSSIVPVDC
                     MSATHDHLPGDANWASQNALDRLFADVRAQRPDTLIFTCRPAQDLGVFSLRNVDVCFT
                     LLETGTGASNLQAGDEIRRWSRYRVHHHFVPHYIDQPLLFPSRADRTTPLVWSSEHLD
                     YILLSAISSSPNLLFYMPTKSGIPDRDKATIRRWLDWARAQEELLKVRRDLPEWPEPD
                     RVDGSAHIVGDRGLVFLFNPGD"
BASE COUNT          356 a          717 c          797 g          313 t
ORIGIN      
        1 ggtcgcccgg gttgaagagg aacacgagcc cgcgatcgcc cacgatgtgc gccgagccat
       61 cgacgcggtc cggctcgggc cactcgggga ggtcgcgccg cactttgagg agctcctctt
      121 gggcgcgtgc ccagtccagc cagcgccgga tggtggcttt gtccctatcg gggatgccgg
      181 acttggtcgg catgtagaac agcaggttcg gcgacgacga gatggcgctc agcaggatgt
      241 aatcgaggtg ctcgctcgac cagacgagcg gcgtggtccg gtcggcccgg ctggggaaca
      301 gcagcggctg gtcgatatag tgcgggacga agtggtggtg gacgcggtag cgcgaccacc
      361 gccggatctc gtcgcccgcc tgcaggttcg aggcgcccgt acccgtctcg aggagtgtga
      421 agcacacatc cacgttgcgc aggctgaaga cgcccagatc ctgcgctggc cggcacgtga
      481 agataagggt gtccggccgc tgggcgcgca catcggcgaa aaggcgatcg agcgcgttct
      541 gactggccca gttcgcgtcg cccggcaggt ggtcgtgcgt ggcggacatg cagtcaacag
      601 gcacgatgct cgagtaccac ccgccgtcgc cgaagaagct accatccatg gcccagccgc
      661 ggtagagccc cgtgctgaag ccctcacgat tgatgcgcga gagccactcg aagaagggcc
      721 ggttcgcgac gcagttgccc ttcgcggacg cgatgatcga gatggcgcgc gacactggcc
      781 cgggcgtgag gagccagtcg ggctggtccg cgcggaaggg tctcccgagc ggcgaccagt
      841 ggtgggtgtt cgtcatcgac gaccacatga cggcgcccat gccgaggtcg cgcgcatgct
      901 cgagcaaccg tcgtgcccgc ggtccagggg tgtagtggtc gtcgggcccg agggcgttca
      961 tccgctcggt ctcgccgccc cacgggtgcg attcggagaa ccagtcgatg ccgcaatcgg
     1021 ccaggaactc cagcgacctc atgtcggcct cgacgtcctc atcggaggca taggggccct
     1081 gctgcatctc cgagtgccac ccgcatgcca tggggacgaa gccgtgacgc ggtggtccga
     1141 gccaggattc ggtcatcgcc accatggcgg ccgactccga agccagaggc acgatcccgg
     1201 gctcggcctc gcgcagttcg accggcgcgc ccgggtccga gggcgagcag cggatcggag
     1261 gcggcagagg acgggtgtcc gcctcgctgc gagcgcacac gcccaggtac atggcctcac
     1321 agggcaccgt ctgcccggcc gataccttca cgctcggcaa gtaggcaagc tcgatggcgt
     1381 tgccgccccc cgtcgaccgg tcgaacggca tggcgaggcc cgcgaacagg ctaccgcgcc
     1441 ctgtccggcc gaagaaggtg ctacacggct cagcgcccgg cacgcggccg aactgcgggt
     1501 agcggtagcg gacgagctcc agcccgggcg cggcgaacgc gggacggctg accgtcactt
     1561 ggcggagggt gtcatcccgg gtgaaggtca gcgcgaggcg cttctcgacg aagtggttgg
     1621 cgggcccgag ggtgtaggac aggtcaatcc gcacaccgtc ggcctcatac ctagccctga
     1681 ccgatgtgcg gtcggcggag agtgacgcgc gccgggcata cgcttgctcg atggtgccgc
     1741 tgccgagctc gatggcgaac tggtcgccct ccaccgtgta gacctcgccg gtcaggcggt
     1801 tacggacggc ggtcagcgca aggggatcgg agcggaactc gaggcggagc gccctgttct
     1861 ggagcacgag gggctctgcc gccgtccagg caacggggat cagcgcagcc acgcagaccg
     1921 cgtgcggcac cgtgggcatg tccatcactc cgcccggctc ttcccccagc gtgcggcatt
     1981 cgcctgccgc tctgtcgagg acgcgaggct tcccacgccg aagactgccg gcgatgctgc
     2041 tggaaggggg cctacgcgtg cgccgccggg agttcttgaa ggcgatggcg gcgggcaccg
     2101 tgctcgcggc gcgcgcgagc gcccaagcca cccgggaggg acaccaatgg aggccgacgg
     2161 cccgaaggtc cagtgggagc tca
//
LOCUS       DAOSPL010000861         2183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_50172_length_2183_cov_2.494831, whole genome shotgun sequence.
ACCESSION   DAOSPL010000861 DAOSPL010000000
VERSION     DAOSPL010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50172_length_2183_cov_2.494831"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PLQ54_14545"
     CDS             <1..246
                     /locus_tag="PLQ54_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94537.1"
                     /translation="ERSPETGPESICGAEIDLCAQDETGELLDLGQIYFGRRHTPPLP
                     TDGWHYYEPAERKQWIWKAKPGRPTPLPERKPGRFEP"
     gene            297..>2183
                     /locus_tag="PLQ54_14550"
     CDS             297..>2183
                     /locus_tag="PLQ54_14550"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HOS94538.1"
                     /translation="MSAKLSLLILGLAACAHAQGERVIAEWTFDAPGDPQGWQPNGHT
                     AGARVADGMLSGRITDWDPFITSPPLDFEATPWQQVEIRARLSKSGPGQVFYTNTLDT
                     PYGGFSPEKHFGIDFIGGDEVRSYRVRPFWQAEKRIIRLRLDLPGEGEFAIDAIRIVD
                     SGPTGEPVEPKWRFARDTLGWQTEQAAVRATDDALEYTSHAPDSLLLGPPVRFAADES
                     PVVAVRMSLDRGARGELVFASDEANGLQRVVFPVRPDGRPHVYNIEACSSAGWRGHII
                     ALALRPVDVEGATVRIESIEAAADAIGPPDLQVGWFGLDDVVNRAGEQCPLAAVITNE
                     GGEVARGVTVRIALPEGVTMEEAPGELDEVEYGFPAQPRWMVRADRPLRGEATLELRA
                     PGQPAVTRTATLEFTAKPDVPKAEHVPVPEPVATDYQVGVYYFPGWHARSRWNPIETF
                     PERKPVLGWYDESLPEIADWQIKWAVEHGVSFFVVDWYWSQGARSLEHWLHGAYMNSR
                     YHEYLKFCLLWANHNPEGSSSHQDCLDVTRYWIDNYFKRPEYLRVDGKPVMVFFSVDR
                     LRNDLGSDGVAQAFEAMDALCRENGIPGIYMVACTAATSEGELRRLADEGYDAVSGYN
                     YPGLN"
BASE COUNT          367 a          697 c          767 g          352 t
ORIGIN      
        1 gaacgctcgc ccgagactgg ccccgagtcc atctgtgggg ccgagatcga cctctgcgca
       61 caggatgaga cgggcgagct gctcgacctg gggcagatct atttcggacg acgccacacg
      121 cccccccttc ctaccgatgg gtggcactac tacgagccgg ccgagcggaa gcagtggatc
      181 tggaaagcca agcccggacg ccccaccccc ctacccgaac gcaagccggg aaggtttgag
      241 ccctagtgac ggacttgcac tcgcatcggc gatcgactgc caaaggacgg tgtgacatgt
      301 ctgcgaagct cagtttgctc atactggggc tggcagcttg cgcccatgcc cagggggaac
      361 gcgttatcgc cgagtggacc ttcgacgcac ccggtgatcc gcaaggttgg cagcccaacg
      421 ggcatacagc cggtgctcga gtcgctgacg gcatgctctc gggccgcatc accgactggg
      481 acccgttcat cacgagtccg cctctcgact tcgaggctac gccgtggcag caggtcgaga
      541 ttcgcgcccg gctctcgaag agcggcccgg ggcaggtgtt ctacacgaac acgctcgaca
      601 ccccgtatgg tgggttctcg ccggagaagc acttcgggat cgacttcatc gggggcgatg
      661 aggtgcggtc ataccgcgtc cgtcccttct ggcaggcgga gaagcgcatc attcggctcc
      721 ggctcgatct acctggcgag ggggagttcg ccatcgacgc gatccgcatc gtcgacagcg
      781 ggcccacagg cgagccggtc gagcccaagt ggcgcttcgc ccgggacacg ctcgggtggc
      841 agacggagca ggccgcggtc cgggcgacgg atgacgcgct cgagtacact tcgcacgcgc
      901 ccgattccct gctcctgggg ccgcccgtgc ggttcgcggc cgatgagagc cccgtggtcg
      961 cggtgcggat gagcctcgac aggggcgcac ggggcgagct cgtgttcgcc agcgacgagg
     1021 ctaacgggtt gcagcgagtc gtgttcccgg tccgccccga cggtcgtcca cacgtgtaca
     1081 acatcgaggc gtgttcgagc gcagggtggc gggggcacat catcgcgctg gccctgcggc
     1141 cggttgacgt cgagggtgcc accgtacgga tcgagtccat cgaggccgcg gcagatgcca
     1201 tcggcccgcc cgatctgcag gttggctggt tcggcctgga tgacgtggtg aaccgggcag
     1261 gcgagcagtg ccctctcgcg gcggtgatca ccaacgaggg cggtgaggtc gcgcggggcg
     1321 tcacggtgcg gatcgcgctg cctgaaggcg tcaccatgga ggaagcgccc ggcgagctcg
     1381 acgaagtcga gtatggcttc cccgcccagc cgcgatggat ggtgagggcc gaccggccgc
     1441 tgaggggcga ggccactctc gagctgcgcg cgcccgggca gccggccgtg actcggacgg
     1501 cgacgctgga gttcaccgcc aagcccgatg tgccgaaggc ggagcacgtc cccgtgcccg
     1561 agccggtggc gacggactac caggtgggtg tgtactactt cccgggctgg cacgcccgca
     1621 gccgctggaa tcccatcgag acctttcccg agcgcaagcc ggtgctcggg tggtatgacg
     1681 agagcctacc cgagatcgcc gactggcaga tcaagtgggc ggtcgagcac ggggtgtcgt
     1741 tcttcgtcgt cgactggtac tggagccagg gcgcccggag cctcgagcac tggctgcacg
     1801 gggcctacat gaacagccgt taccatgagt acctgaagtt ctgcctgctg tgggcgaacc
     1861 acaacccgga gggctcgagc tcacaccagg actgtcttga tgtgactcgg tactggattg
     1921 acaactactt caagcggccc gagtacttgc gcgtggatgg caagcccgtg atggtgttct
     1981 tctccgtcga ccgcctgcga aacgacctgg gcagcgacgg cgtcgcccag gccttcgagg
     2041 cgatggacgc gttgtgcagg gagaatggca tcccgggcat ctacatggtg gcatgcacgg
     2101 ccgcgacgtc cgagggagag ctgcggcgct tggccgatga gggttacgat gccgtgtcgg
     2161 gctacaacta cccgggactg aac
//
LOCUS       DAOSPL010000862         2183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_50176_length_2183_cov_2.289004, whole genome shotgun sequence.
ACCESSION   DAOSPL010000862 DAOSPL010000000
VERSION     DAOSPL010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50176_length_2183_cov_2.289004"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PLQ54_14555"
     CDS             complement(<1..892)
                     /locus_tag="PLQ54_14555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS94539.1"
                     /translation="MMKCGAGLISALFLLGFAPADLFKSRAQVLAERASGEYASGDYR
                     AALDGYSQAAQAAPQSPELEFNRGAAAYKAGDYATANAAFAHAAKLAGSTSGLAVKAH
                     FNSGNAYFKQGQYEQAIREYDAVLQSAPGDEDAAFNKKLAEEALKRQRQQPQQQQQQQ
                     QQQQQQQQQQQQQQQEEKQQKGQEPRGQQREEQSQPDQSAGDASRQQPESRRHPQQQS
                     EPQEQQQGRRAYSNPVQPPPAAGRQYPQLDPADVDRALKQLEQLERYGRRRLNPDPKR
                     PRQSPSILDDLDQLLPPGFFQ"
     gene            complement(889..1905)
                     /locus_tag="PLQ54_14560"
     CDS             complement(889..1905)
                     /locus_tag="PLQ54_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOS94540.1"
                     /translation="MGFGQPQWLWGLIAAPIGVAASLLWWFADRRRGAAFADPALLAR
                     VMGGRRSRATLKTVMVLVALLLTVAAAARPQVGHEWQTALGSGVDVVVAIDVSTSMRA
                     ADAGFARSRLYGALAIVDRLVSRLEYDRVALVTFTDEATTICPMTADMGAVLTMLDGV
                     DYESVGEGGTALAAAVREACERFDPKESRGRVVIILSDGEDQEQGVEEAVAEAKRLGA
                     VVHTVGIGTTEGARIPLGSGLFGGTVYKEYQGQTVVTRLDEAALKRVAEGTGGTYRHA
                     TAESTADGLVGVIRAAAGKGRTVRTVTTAREAFQPLIALALVLLIVEALVPSRSVGRR
                     PVAP"
     gene            complement(1910..>2183)
                     /locus_tag="PLQ54_14565"
     CDS             complement(1910..>2183)
                     /locus_tag="PLQ54_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOS94541.1"
                     /translation="TDQFGRPIFTRLDEATLTEIARITGGQYYRAETNERLSQVLATI
                     AQLEKSEVVVKRSKVMKEQAQWLVVPAVLLLAIEFAAFCGWLRVMG"
BASE COUNT          328 a          805 c          646 g          404 t
ORIGIN      
        1 cctggaagaa cccaggcgga agcagctgat ccagatcgtc gagaatcgac ggcgactgcc
       61 tcggtctctt ggggtcgggg ttgagccgtc tccggccata gcgctccagc tgctcaagct
      121 gcttcagcgc ccggtccacg tcggccggat cgagttgcgg gtactggcgg cccgccgcgg
      181 ggggcggctg gactggattc gaataggccc gccggccctg ctgctgttcc tgtggttccg
      241 attgctgctg tgggtgtcgt cgcgattccg gctgttggcg tgacgcatcg ccagccgact
      301 ggtctggctg gctctgctcc tcccgctgct gcccacgcgg ctcctggccc ttctgttgct
      361 tctcttcctg ctgttgttgt tgttgctgct gttgctgctg ctgttgttgt tgctgttgtt
      421 gttgttgctg ctggggctgc tgcctctggc gcttgagggc ctcttcggcc agcttcttgt
      481 tgaacgccgc gtcctcgtcg ccgggggcgg actggagtac ggcgtcatac tcccgtatgg
      541 cctgctcata ctggccctgt ttgaagtagg cgttccccga gttgaagtgg gccttcacgg
      601 ccaggcccga ggtactgccg gccagcttag cggcgtgggc gaaggctgcg ttcgccgtcg
      661 cgtagtcgcc cgccttgtac gccgcagcgc cgcggttgaa ctcgagctcc ggagactgag
      721 gagccgcctg agcggcttgg ctgtacccat ccagcgccgc ccggtagtcg ccgctcgcat
      781 actctcccga tgcccgctct gcaagcacct gggcccgcga cttgaacagg tcggccggag
      841 cgaaacccaa caagaacaag gcactgatca accctgcacc gcacttcatc atggcgccac
      901 cggcctcctc ccgaccgaac ggctcggcac cagcgcctct acgatcagca gcaccagggc
      961 gagggcaatc aagggctgga atgcctcgcg ggccgtcgtc accgtccgga cggttcggcc
     1021 cttgccggcg gccgcccgga tgaccccaac caagccgtcc gccgtgctct ctgcggtcgc
     1081 atggcggtac gttccacccg tgccctcagc cactcgcttc agagcggcct cgtccagccg
     1141 ggtcactacc gtctggccct ggtactcctt atacaccgtc ccgccaaaga gccccgaacc
     1201 cagtggaatc cgagcccctt ccgttgtgcc gatgccgacc gtatgcacaa ccgcgccgag
     1261 ccgcttcgct tccgccacgg cctcctcaac gccttgctcc tggtcctcgc cgtcgctgag
     1321 gatgatcacc acccggccgc ggctctcctt gggatcgaac cgctcacagg cctcacggac
     1381 tgccgccgct agcgccgtgc ccccttcgcc caccgactcg tagtccactc catcgagcat
     1441 ggtgagcacg gctcccatgt ctgccgtcat cgggcatatc gtggtcgcct catcagtgaa
     1501 ggtcaccagc gcaacgcggt catactccag gcgcgatacc aggcggtcca cgatggcaag
     1561 cgccccgtac agacggctgc gcgcgaaacc cgcgtccgcc gccctcatgc tcgtggatac
     1621 atcgatggcc accaccacgt cgactcccga gcccagagcc gtctgccact catgccccac
     1681 ctggggccgc gccgcggcgg ccacggtgag cagcagcgcc accagcacca tcaccgtctt
     1741 cagcgtcgcc cgcgaccgtc gcccccccat gacccgggcg agcagggcgg gatcggcgaa
     1801 agccgcgccg cgacggcggt ccgcaaacca ccacagaagt gaggccgcca ccccaatcgg
     1861 tgccgcgatc agtccccaga gccactgtgg ctggccgaaa cccatcgccc taccccatca
     1921 cgcgcagcca accgcagaac gcggcgaact cgatcgccag gagcagcacc gcgggaacca
     1981 ctagccactg cgcctgctcc ttcattacct ttgacctctt gaccacaacc tcagacttct
     2041 cgagctgcgc gatggttgcc agaacctgac tcagtctctc gttggtctcc gctcggtagt
     2101 actgtccgcc cgtgatccgc gcaatctccg tgagcgtcgc ttcgtccaga cgcgtgaaga
     2161 tgggcctccc gaactgatcg gtg
//
LOCUS       DAOSPL010000863         2180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_50274_length_2180_cov_2.700706, whole genome shotgun sequence.
ACCESSION   DAOSPL010000863 DAOSPL010000000
VERSION     DAOSPL010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50274_length_2180_cov_2.700706"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PLQ54_14570"
     CDS             complement(<1..674)
                     /locus_tag="PLQ54_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94542.1"
                     /translation="MGAVVLCVSFVGSQERGRSADYSEIREIASAFVSALTSAGLDNF
                     KGLAFPCYLVMPGMLDEVTEFKSETDLAPPGDRVKFEKRDMAVRLAGKAAVCTVQFRP
                     TGSPQWATAVLLLTRPAGFWVVRAIAASPNQLAADSDLDMVGALQSDFVRCITTNGPA
                     VFDGLDFPLIFVNGRTGTVRLYDAPEDLPPAGHSLGLRSRRLRWAYADNIAACTMELT
                     RADEVAA"
     gene            complement(893..1186)
                     /locus_tag="PLQ54_14575"
     CDS             complement(893..1186)
                     /locus_tag="PLQ54_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94543.1"
                     /translation="MVWCRKYRKKIAEFRCETCSDAVKVQNQHLCGRPFESPVEAEPA
                     KARPKRAAEAEGEAAARRHGRRRRGRVVRMREKRSASGRKRGAAPPQNPGSSS"
     gene            complement(1322..>2180)
                     /locus_tag="PLQ54_14580"
     CDS             complement(1322..>2180)
                     /locus_tag="PLQ54_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94544.1"
                     /translation="EVTLAGEPDPSYTFRVGQGVELDASARETLDRYIAGVRDATWWG
                     DGGAAASALQAIDPGYSYNGTDAHGALWASGIGSAEDTTLAVTASSAALSQGAYAVML
                     DAIQDSTWTYPAYDGGDVAPLSAEEDTDGDPSLIDDADEPVDAPDRGMGCSPYWYGNR
                     HMRLLFELNPQTWKITAIRVVLAWGGEDDAPAPTANLTVNGAETATVAPGVPVTIAGD
                     LGGSVIEGASVSLGWQGEWVEAAGQSFSLSIPAPEEPGRWVATAYVWGSAYGQEVTIS
                     REIVVASTP"
BASE COUNT          382 a          764 c          684 g          350 t
ORIGIN      
        1 gcggcaacct cgtcggcgcg ggtgagttcc atcgtgcacg cggcgatgtt gtccgcatac
       61 gcccatcgga gccgccgcga tcgcaggccc aacgaatgcc cggccggggg aaggtcctcc
      121 ggggcgtcgt acaaccggac cgtacccgtc cgaccgttca cgaagatcag cgggaagtcg
      181 agcccatcga acaccgcagg cccgttggtg gtaatgcatc gcacgaagtc ggactgcagg
      241 gcgccgacca tgtccagatc actatcggcc gccagctggt tcggcgaggc tgcaatggcg
      301 cgcacaaccc agaacccggc cggccgggtc agtaggagaa cggcggtggc ccactgcgga
      361 gacccagtgg gccggaactg aaccgtgcac acggctgcct tgcctgccag ccgaaccgcc
      421 atatcgcgct tctcgaactt cacgcggtct ccgggcggcg cgaggtctgt ttccgacttg
      481 aactcagtga cctcgtcgag catgccgggc atcacaaggt agcaggggaa ggccagaccc
      541 ttgaagttgt ccaggcccgc gctggtcaga gccgagacga acgccgaagc gatctctcgg
      601 atctcggagt agtcggcgga tcggccacgt tcttgagaac ccacaaacga gacacagagg
      661 accacggcgc ccaagcaaca gactgtcccg agacccgttc tcctcatgac ccgtccccac
      721 gaccggccct atgggcccgg tgctgacgaa cccgagaatc ggactgggcg ctagccggag
      781 ctatggcggc aaccaggccc gggggccatc acccgcacac accgattcaa tgctacgtac
      841 tggcgggttg gagggggtcc tgctacttgg cgtagttttt acattcaggg catcaggagg
      901 acgatccagg attctgaggt ggggcagccc cgcgcttgcg gccggacgcc gaacgcttct
      961 ctctcatacg caccactcgc ccgcggcgcc ggcgcccgtg acgacgtgcc gcagcctcgc
     1021 cctcggcctc cgccgcccgc ttcgggcggg ccttggccgg ctccgcctca acgggcgact
     1081 cgaatggccg gccgcacaga tgctgattct gcaccttgac ggcatccgag cacgtctcgc
     1141 acctgaactc ggcaatcttc ttgcggtact tacggcacca gaccatgtcg actctctcac
     1201 ccgcggtggg tcggaactgc actggacctc agtatagccg aaggggagct cgcgacccaa
     1261 acggacggag ggacgccacc gaggcgtccc tccttggggc actcctgacc acgcgccctg
     1321 actacggcgt gctggcaacc acgatctcgc ggctaatggt cacttcctgg ccataggcac
     1381 tgccccacac ataggccgtg gccacccatc gccctggctc ttcgggggcc gggatgctga
     1441 gtgagaaact ctggccggcc gcctcaaccc actcgccctg ccaccccagg gacacactcg
     1501 ctccttcaat gacagaaccg ccgaggtcgc cggcgatcgt gaccggcact cccggtgcca
     1561 ccgtggccgt ctccgctccg ttgaccgtca ggttcgccgt tggcgccggc gcgtcgtcct
     1621 cgccacccca ggccaatacg acccgaatgg ccgtgatctt ccaggtctgg gggttgagct
     1681 cgaagagcaa tctcatatgg cgattaccat accaatacgg agaacatccc atgccccgat
     1741 ccggcgcatc gaccggctcg tctgcatcat cgatgaggct cggatcgccg tcggtgtcct
     1801 cctcggcgga gaggggcgcg acatcgcccc cgtcataggc cggataggtc catgtgctgt
     1861 cctgaatggc gtcgagcatg actgcatagg ccccctggct cagggcggcc gacgacgccg
     1921 tgaccgcgag agtggtgtcc tcagccgagc cgatacctga agcccatagg gcaccgtggg
     1981 cgtcggtccc gttgtagctg tagccggggt cgatggcctg cagtgcactg gcggccgcgc
     2041 ccccgtcacc ccaccaggtg gcgtcacgca cgccggcgat gtaccggtcc agggtctccc
     2101 gggccgaggc atcgagttcg acgccctggc ccaccctgaa cgtgtacgac ggatcgggtt
     2161 cgcccgcgag ggtgacctcg
//
LOCUS       DAOSPL010000864         2177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_50367_length_2177_cov_3.572102, whole genome shotgun sequence.
ACCESSION   DAOSPL010000864 DAOSPL010000000
VERSION     DAOSPL010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50367_length_2177_cov_3.572102"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLQ54_14585"
     CDS             complement(<1..239)
                     /locus_tag="PLQ54_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOS94545.1"
                     /translation="MSKALVTGGAGFIGSHIVDRLLGMGFDVAVIDNLSTGYRRNLNA
                     KATFHERDIRDGEAVRAIFEAERPSVVFHLAAQMDV"
     gene            complement(298..1278)
                     /locus_tag="PLQ54_14590"
     CDS             complement(298..1278)
                     /locus_tag="PLQ54_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94546.1"
                     /translation="MPTPDGKQWGRLRLFDPRKGVTIRAPLGSAPGWWAGASSCVREH
                     STGFFFMYYRLRKPRERGRGAECRIAASADGVHFEDVWHATRDQIGTVSMERSALVQG
                     EDGVWRLYLSYVDPADNRWRIDLLEADEPRRFDPQIRQPVLTGPTIGAEGVKDPVVLV
                     FGGIYFMFVSYARPPVGLEPHQAAGLHATADVFNTGLTLSATGLALSSDGRHFEWQGE
                     VFGPAPSGWDRYCARISTVVYLPPVFAFLYDGSATVDENYEERCGLGMSLDLARFHRA
                     SANQPVLTSPHGSGSLRYADAVRDGDRLLLYYEYARPDGSHELRMSLVTF"
     gene            complement(1521..2093)
                     /locus_tag="PLQ54_14595"
     CDS             complement(1521..2093)
                     /locus_tag="PLQ54_14595"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOS94547.1"
                     /translation="MESLGNRDQIRIVTQPLAKNLLIFAGPGSGKTRTIVHRCAYLLR
                     VKRVRPRAILVCCFNHRAALELRRRLGALVGRDALGVTRIRSLVNLTDVRVPIAIGVA
                     ALDILEAHLTGASGLPVPAIAIEPHIGLVGFIFPFRDEMADRTAAELRRTRMVRALEQ
                     LHVGAGGRRGGRHAQSDQDCQRRHSSGACA"
BASE COUNT          398 a          723 c          696 g          360 t
ORIGIN      
        1 acatccatct gtgcggccaa gtggaacacc acactggggc gctcggcctc gaagatggcc
       61 ctcacagcct ctccgtcacg gatgtcccgc tcgtggaagg tcgccttcgc gttcaggttc
      121 cgccgatagc cggtcgacag gttgtcgatc acggccacat cgaatcccat gccgagcagc
      181 cggtccacga tgtgcgaacc gatgaagccg gcgccccccg ttaccagtgc cttagacatc
      241 agactacccc cagttcccgc atccgatggc cgcaggcctg cgtgccacgg ctccccgcta
      301 gaacgtcacc aggctcatcc gcagttcgtg cgatccatcg ggccgcgcgt actcgtagta
      361 caggagcaac cggtcaccat cgcgcaccgc atcggcatag cggaggctcc ccgagccgtg
      421 gggcgatgtg aggaccggct gattcgccga ggcacgatgg aaccgggcca ggtccagcga
      481 catgcccagt ccacaccgct cctcgtagtt ctcgtccacg gtggcgctcc catcgtagag
      541 gaaggcgaag acgggaggga ggtacacgac cgtactgatc cgagcacagt agcggtccca
      601 acccgaaggg gccgggccga acacctcgcc ctgccactcg aagtggcgtc catcggagct
      661 gagcgccagg ccggtggcgg acagtgtcag gcccgtgttg aacacatcgg ctgtggcatg
      721 cagaccggcg gcctgatggg gctcgaggcc gactggtggc cgagcgtagc tcacgaacat
      781 gaagtagatc ccgccgaaca ccagcaccac ggggtccttg accccctcgg cgccaatggt
      841 tggtccggtg agcactggct gccggatctg cgggtcaaag cgccgtggct catccgcttc
      901 gagcaggtcg atccgccacc ggttgtcggc agggtccaca tagctgaggt agaggcgcca
      961 gactccgtcc tcgccctgga cgagagccga tcgctccatg gaaacggttc cgatctggtc
     1021 gcgcgtcgcg tgccaaacat cctcgaagtg gactccgtcg gcgctcgcgg cgattcggca
     1081 ctcggctccg cggccccgtt cccgcggctt gcggaggcga tagtacatga agaagaaccc
     1141 cgtgctatgc tcgcggacgc acgagctggc acccgcccac caccctgggg ctgaccccaa
     1201 cggtgcgcgg atggtgacac ccttgcgcgg atcgaagagg cgcaggcgtc cccactgctt
     1261 gccatccggg gtaggcatcg ctggccacat ccgatccctt cttcccgatc ccgagatcca
     1321 ttccgcctcc tccgccggtc gtcctctggc tcctcgtgcg cgaaccgcga ccgatccagc
     1381 cctcttgagc gtctgataga gtggccggtt cgcctgccgg gcaggtaatg gacggcgaac
     1441 ggcaaactat ggagtcgtcg ggcagggcct gtgacaccag ttggctcggc actcgaggag
     1501 agatgcccat gaccgccgcc ttacgcgcac gcccccgagc tgtggcgccg ttggcagtcc
     1561 tgatcgctct gggcgtggcg accgccgcgt ctgccaccag ctccaacatg aagctgttca
     1621 agcgcacgta ccattcgcgt tcgccggagc tcggccgctg ttctgtctgc catctcgtcc
     1681 cggaagggga agatgaaccc aacaaggccg atgtgaggct caatggctat ggccgggact
     1741 ggaagaccgc tggcgccagt gagatgggcc tcaaggatat cgagggccgc gactccgatg
     1801 gcgatgggta ctcgaacatc cgtgagattc acgagggatc gaatccgggt gacgcccagc
     1861 gcgtcccggc cgaccaaagc gccgagacga cggcgcaact cgagggcggc ccggtgattg
     1921 aagcaacaga ccagaatcgc gcggggacgc acccgtttga cccgaagcag gtaggcacac
     1981 cgatgcacga tggttcgggt cttcccggaa cccgggcccg cgaatatcag gagatttttg
     2041 gccaggggct gggtgacgat gcggatctgg tcgcgatttc cgagcgactc cacgatggcc
     2101 tgatacgaac gggcggtggt ggcccgttcc agcaactcgc gggtgtcgcc aaaaaattgt
     2161 cggatgaagt cttcctt
//
LOCUS       DAOSPL010000865         2176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_50402_length_2176_cov_3.000000, whole genome shotgun sequence.
ACCESSION   DAOSPL010000865 DAOSPL010000000
VERSION     DAOSPL010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50402_length_2176_cov_3.000000"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PLQ54_14600"
     CDS             <1..863
                     /locus_tag="PLQ54_14600"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOS94548.1"
                     /translation="VIESAEVRLGEGVCGHVAATGEPLLITDIRRDPRFAPGERHECY
                     NGPSLLSVPLKFRGSVTGVLNLSNKTDGARFSPADEELLCLLASQAAVAIENAALYRS
                     LCELAATDGLTGLYNHQHFFSRLRGAVSLARRSGQPLAVIMIDIDHFKRVNDRFGHAQ
                     GDTVLRAVASLLRGAARKEDIAARYGGEEFAVILPNTGAQGATHLAERFRARVQELEF
                     RRDDRSFSITVSLGIADLEPGLDPEVLLDRADQALYAAKSQGRNRTCVYEGTATAAPV
                     APEPEGDPIN"
     gene            865..1668
                     /locus_tag="PLQ54_14605"
     CDS             865..1668
                     /locus_tag="PLQ54_14605"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOS94549.1"
                     /translation="MRRPRFDLLALDIDGTLLDDHFAIPPRHREAVCAAKEAGVQIAL
                     VSGRALPAVRPYYAELGIEGLAVSCNGARVTRVPQGGDLFHRPLEPRHAEAIIRECEA
                     RDLHLNVYVGDHLYAREYTPWARLYHERTGSRCEEHPDLASLAAAGPTKLLIVSDSNT
                     GARLADEFRARFGGELYVTLTMPEYVEFMHRDVSKADGLAKTAQALGIDRSRVAAAGD
                     SYSDISMIQWAGLGIAMANGVEPCKAVADVVAPPSSDNGLAWAIEQYVL"
     gene            1762..>2176
                     /locus_tag="PLQ54_14610"
     CDS             1762..>2176
                     /locus_tag="PLQ54_14610"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS94550.1"
                     /translation="MRYRRLGRTGLRVSELSYGAARGASEDPKQFIATVHACLDAGVN
                     FIDSAAGYDGGLSETVLGEALQGYDDVFVETKYCPYESYAVGAAYIGTPQALVASAEE
                     SLRRLRRDCVDVFLGHGIRTLDTLDRFMNDGCYDAM"
BASE COUNT          364 a          817 c          662 g          333 t
ORIGIN      
        1 aggtgatcga gtccgccgaa gtgcgcctgg gcgaaggggt gtgcgggcat gtggccgcca
       61 ccggagaacc cctgctgatc accgacatcc ggcgcgaccc acggttcgcg cccggcgaac
      121 gccacgagtg ttacaacggc ccctccctgc tctcggtacc cctcaagttc cggggcagcg
      181 tcacgggcgt gctcaacctg tccaacaaaa ccgatggcgc ccggttctca ccggccgacg
      241 aggaactgct gtgcctcctg gcaagtcagg cagccgtggc catcgagaac gccgccctct
      301 accggagcct ttgcgagttg gcggccaccg atgggctgac cggcctctac aaccaccagc
      361 acttcttctc gcggcttcgc ggcgccgtga gcctggcccg acggtcaggc caacccctgg
      421 cggtcatcat gatcgacatc gaccacttca agcgggtgaa cgaccggttt ggccacgcgc
      481 agggcgatac cgtgctccgc gcggtagcct ccctgctccg cggggccgct cgcaaggagg
      541 acattgcggc ccggtatggg ggcgaggagt tcgccgtaat cctccccaac accggggccc
      601 agggcgccac ccacctggcc gagcgcttcc gggcccgcgt gcaggaactc gagttccgcc
      661 gcgatgaccg ctcgttctcg atcacggtca gcctcgggat cgccgacctc gagcccggcc
      721 tcgatcccga agtgctgctc gatcgcgccg atcaggctct atacgccgcc aagagccaag
      781 gccgcaaccg cacgtgcgtc tacgagggca ccgcaaccgc cgcccccgtg gcgcccgagc
      841 cggaaggaga tccgatcaac tagcatgcga cgtccccggt tcgacctgct ggccctcgac
      901 atcgatggca ccctcctcga cgaccacttc gccatcccgc cacgccaccg agaagccgtc
      961 tgtgccgcaa aggaggccgg cgttcagatc gccctggtct ctggccgcgc tctgccggcg
     1021 gtccgcccct actacgccga actcggaatc gagggcctgg ccgtctcctg caacggcgcc
     1081 cgcgtcaccc gcgtccccca gggcggcgac ctgttccacc gaccgctcga gccccgccac
     1141 gctgaagcca tcatcaggga gtgtgaggcc cgcgatctcc acctcaacgt ctatgtcggc
     1201 gaccacctgt acgcccggga gtacacgccc tgggcgcgcc tctaccacga gcgcaccggg
     1261 tcccgctgcg aggagcatcc cgacctggcg tctctggctg ccgccggtcc caccaagttg
     1321 ctcattgtga gcgactccaa caccggagcc cgtctggcgg atgagttccg cgcccggttc
     1381 ggcggcgaac tctatgtcac cctcaccatg cccgagtacg tcgagttcat gcaccgcgat
     1441 gtcagcaagg ccgacggcct cgccaagacc gcccaggccc tgggcatcga ccggtcccgt
     1501 gtcgccgccg ccggcgacag ctacagcgac atcagcatga tccagtgggc cggactcggc
     1561 attgccatgg ccaatggtgt cgagccatgc aaggccgttg ccgacgtcgt cgctcctccc
     1621 agcagcgaca atggcctcgc ctgggccatt gagcagtacg ttctgtagcg tggcgtcctt
     1681 ctcgctggaa ggcatcggcc caaaccctga cgaaggaact caagccggat cgacacgcag
     1741 gttccgagag gggcagacac catgcgctat cgccgcctgg gacgaaccgg gctgcgcgtc
     1801 tccgaactga gctacggcgc ggcccgcggc gccagcgagg accccaagca gttcatcgcc
     1861 accgttcacg cctgcctgga cgccggcgtc aacttcatcg acagcgccgc ggggtacgac
     1921 ggcggcctga gcgagaccgt tctcggcgaa gccctgcagg ggtacgacga cgtcttcgtc
     1981 gagaccaagt actgccccta tgaaagctac gccgtcggcg ccgcctacat cggcacgccc
     2041 caggcactcg tcgcctcggc cgaggagagc ctccgccgtc tgcggcgcga ctgcgtcgac
     2101 gtgttcctcg gccacggtat ccgcacactc gataccctcg accgcttcat gaatgacggc
     2161 tgctacgacg ccatgt
//
LOCUS       DAOSPL010000866         2173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_50507_length_2173_cov_2.572238, whole genome shotgun sequence.
ACCESSION   DAOSPL010000866 DAOSPL010000000
VERSION     DAOSPL010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50507_length_2173_cov_2.572238"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="PLQ54_14615"
     CDS             complement(<1..157)
                     /locus_tag="PLQ54_14615"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOS94551.1"
                     /translation="MTDRRVDVLIAGAGAAGLRAALAAREAGAERVLIVSKTESGVAN
                     CSGIAWGG"
     gene            complement(222..1037)
                     /locus_tag="PLQ54_14620"
     CDS             complement(222..1037)
                     /locus_tag="PLQ54_14620"
                     /inference="COORDINATES: protein motif:HMM:NF037833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-type lectin-domain containing protein"
                     /protein_id="HOS94552.1"
                     /translation="MHRSHMRVLSGALLIVLCLAGAASAQVPGDWTLVGSASRDGGKI
                     VITPDEGEQIGAAWYSNPVDLSRDADLRFRVYLGDKDEEGADGLTFILAAERGGTDAG
                     QGLGYQGVEKSLAIEIDTYPNDGEHLGDDLADPAADHVTVDIDGSVDHHDSDRPLAEL
                     DNIEDGREHVLRIQWVAAEKMLRVFIDDMETPKIEYGADIVADRLGGQSAARLGVAGS
                     TGMATNRQYFIVQKLAVGEIDGGGGQAGGGEAGGETDGWIVCPNCGHRFKLDQ"
     gene            complement(1080..>2173)
                     /locus_tag="PLQ54_14625"
     CDS             complement(1080..>2173)
                     /locus_tag="PLQ54_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94553.1"
                     /translation="ESNVVYRTVSGGFSIHYGQELTARNNIFAFGRDYQVSRGRIDKE
                     TSFVFERNIVCYESEALYTGSGKVTADYNLYYRTKGEGLAFPDELTLAEWQAAGQDTH
                     SVVADPLFVDATAGDFRLRPGSPALALGFQPIDTSQCGLTEPPSLVALADAIRTPPLR
                     LAERPKPKPLLIDEGFEDSLPGVAAENAVTWGEAGKARVRVTDQAAAEGRHALRFTDG
                     PGLDYPFNPHIWYTPNIEKGLVTVQFDLRIGEGALAWIEWRDAASPYRVGPGLGTNVD
                     GQLSAGGKPVMPMPIGEWVHIAATCRIGREAPGRWDLEVTASGAEPVRMTDLPCDPKF
                     RKLEWLGFISNSQTDTVFDIDNIRITVRP"
BASE COUNT          332 a          776 c          686 g          379 t
ORIGIN      
        1 gcccgcccca cgcgatgcca ctgcagttgg cgacgccact ctctgtcttc gagacgatca
       61 gaacccgctc ggcccccgcc tcgcgcgcgg ccagggcggc cctcaaacca gccgccccgg
      121 ccccggcgat cagtacatcg acccgtctat ctgtcatggc caaccctccc gagtatctga
      181 cgcccgggag gaccggcgcg aaggacccgg ttcccgcatc gctactggtc gagcttgaac
      241 cgatgcccgc agttggggca gacgatccag ccatcggtct cgccacccgc ctcgccaccg
      301 cccgcttggc cgcctccgcc atcgatctct cccaccgcca gcttctgcac aatgaaatac
      361 tggcggttcg tcgccatgcc ggtggacccg gcgacgccca gccgggctgc tgactgcccg
      421 ccgagccgat cggccacgat gtccgcaccg tactcgatct tcggtgtctc catgtcgtcg
      481 atgaacacgc ggagcatctt ctccgccgcc acccactgga tccgcagaac gtgttcccgc
      541 ccgtcctcga tgttgtcgag ctcggccagg gggcggtcgg agtcgtgatg gtctaccgac
      601 ccatcgatgt cgaccgtcac gtggtctgcc gccgggtcgg ccagatcgtc gcccaggtgc
      661 tcgccgtcgt tcgggtaggt gtcgatctcg atggctagcg atttctcaac gccctgatag
      721 ccgaggccct ggccggcatc ggttccgcct cgctcggcgg cgaggatgaa ggtcaggccg
      781 tccgcgccct cttcgtcctt gtcgcccagg tagacgcgga accgcaggtc ggcatcgcgg
      841 ctcaggtcga ccgggttcga ataccaggcg gcgccgatct gttccccctc gtccggtgtg
      901 atgacgatct tgccgccgtc gcggctcgcc gagcccacca gggtccagtc gcccggcacc
      961 tgcgccgacg cggcgccggc caggcacagc acgatgagga gtgctccact gagtacacgc
     1021 atatgactac ggtgcatccg gtcggtccct ccagttcagt aacggctcgc gcatgcgagt
     1081 tagggcctca cagtgatgcg gatgttgtca atgtcgaaga ccgtgtcggt ctggctgttg
     1141 ctgatgaacc caagccactc cagcttccgg aacttcggat cacacggcag atcggtcatg
     1201 cgcaccggct cggcgcccga tgcggtcacc tccaggtccc atctcccggg agcctcccgc
     1261 ccgatccggc aggtcgccgc gatgtggacc cactccccga tgggcatggg catcacgggc
     1321 ttcccgccgg cggagagctg cccatcgacg ttcgtgccca ggccgggccc gacgcgatag
     1381 ggcgaagccg cgtcgcgcca ctcgatccag gcgagagccc cctccccgat ccgcaggtcg
     1441 aactggaccg tcaccaggcc tttctcgatg ttcggtgtat accagatatg tgggttgaac
     1501 gggtagtcga gcccgggccc atcggtgaac cggagggcat gcctgccttc ggcggcggcc
     1561 tgatcggtga cgcgaacccg tgccttgccg gcctcgcccc acgtgaccgc attctcggcg
     1621 gccacgcccg gcagcgagtc ctcgaagccc tcatcgataa gcaggggctt cggctttggc
     1681 cgctcggcga ggcgaagcgg cggcgtccga atggcgtcgg ccagcgccac caggctgggt
     1741 ggctcggtga ggccacactg gctggtatcg atgggctgga agcccagcgc cagcgccggc
     1801 gatccgggcc gcagccggaa gtcgccggcc gtggcatcga cgaacagtgg atcggccacc
     1861 acggagtgcg tgtcttgccc tgcggcctgc cactcagcga gggtcagctc atcgggaaac
     1921 gcgagcccct cgcccttcgt ccggtagtag aggttatagt cggcggtcac cttgcctgac
     1981 cccgtgtaga gcgcctcgct ctcgtagcag acgatgttgc gctcgaagac gaagctcgtc
     2041 tccttgtcga tacggccccg gctgacctgg tagtcccgcc cgaaagcgaa gatgttgttg
     2101 cgcgcggtta gctcctggcc gtagtgaatc gagaacccgc cgctgacggt gcggtagacc
     2161 acgttgctct cat
//
LOCUS       DAOSPL010000867         2171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_50561_length_2171_cov_3.112004, whole genome shotgun sequence.
ACCESSION   DAOSPL010000867 DAOSPL010000000
VERSION     DAOSPL010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50561_length_2171_cov_3.112004"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /locus_tag="PLQ54_14630"
     CDS             complement(<1..1422)
                     /locus_tag="PLQ54_14630"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOS94554.1"
                     /translation="MNRLTLSLLCLTFATPLLAQGGGPPGETPEQRDARTKWFREAKF
                     GVFICWGPCSIAEGEIGWSRGGPRPGVGGTGEVPLEVYDNLYKRFNPTKFNAREWAKL
                     VKDAGARYVIFLTKHHDGFCMFDSKLTDYKVTRTPFGRDVTAELAQAFHEAGIRIFWY
                     YSQPDWHHPDYLTENHARYIDYFHGHVRELLTRYGRIDGMWFDCLQGTAETWDTPRLF
                     AMIRTLQPGILINNRGGWPGDFDTPEQTIGTFQIDRPWESCMTMSTGWSWLGEKAPVK
                     SLTECLHLLIRCAGGGGNLALDTGPMPDGRIDPRQAANYRGMGQWLRRYGESIYATTG
                     GPYTPSRSMVSTRAGNRVYLHVLNWDEDDLVLPPIPAKVIACSALTGGTPMCTQSADA
                     LRIRLRPERRDPTDTIIRLELDSSAEAIRPISIRAQQSLAVGKRASASGIWNDDYSAQ
                     KAFDGDEATRWGCTPDSRSGWLEV"
     gene            complement(1462..>2171)
                     /locus_tag="PLQ54_14635"
     CDS             complement(1462..>2171)
                     /locus_tag="PLQ54_14635"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOS94555.1"
                     /translation="LEFLRGVGIRVLKGCTHCRIIGNAVHSIGASGIMVGENGNLADP
                     VVPRGNRVENNVVHDCGLDDYGAVGIWVAFTNGTRVAHNLVYNLPYTGISVGFQWDES
                     PTCCANNRIEYNHVHHVLQRLCDGGCIYTLGLQPGTVLRGNWLHDALRAGSAQGAPNN
                     GIFFDQGSKGFTIVDNLIHATSAEPIRFNQCAADWHTFEANWFGDEAAGDPAADRIRD
                     RAGLESPHRERLSPPAP"
BASE COUNT          335 a          708 c          751 g          377 t
ORIGIN      
        1 cacctcgagc cagccgctac ggctgtccgg cgtgcagccc cagcgggtgg cctcgtcgcc
       61 gtcgaacgcc ttctgagcgc tgtagtcatc gttccagatc cccgaggccg aagcccgctt
      121 cccgacggcc agcgactgct gggcgcggat ggagatgggc cggatcgcct ctgccgagct
      181 gtcgagctcg agccggatga tcgtgtcggt tgggtcgcgg cgctcggggc gcaggcggat
      241 gcgaagggca tctgccgact gggtgcacat cggcgtgccg cccgtcagcg ccgaacaggc
      301 gatcaccttc gccggaatcg gcggcagcac caggtcgtct tcgtcccagt tgagcacgtg
      361 caggtagacg cggttgccgg cacgggtcga gaccatactg cggctcgggg tgtaggggcc
      421 gccggtggtg gcatagatgc tctcgccgta acgacgcagc cactggccca tgccgcggta
      481 gttggcggcc tggcgcggat cgatgcggcc gtcgggcatg ggacccgtat cgagcgccag
      541 gttcccgccg ccgccggcac agcggatcag caggtgcagg cactccgtga gcgacttcac
      601 gggggccttc tcacccagcc acgaccagcc ggtgctcatg gtcatgcagc tctcccaagg
      661 gcgatcgatc tggaaggtgc cgatggtctg ctcgggtgtg tcgaagtccc ccggccagcc
      721 gccgcggttg ttgatgagga tgcccggctg cagcgtgcgg atcatcgcga agagcctcgg
      781 cgtatcccac gtctccgcgg tgccctggag gcagtcgaac cacatgccgt cgatcctccc
      841 gtagcgggtg agcagctccc ggacgtggcc gtggaagtaa tcgatgtagc gcgcatggtt
      901 ctcagtcagg tagtccggat ggtgccaatc cggctgggag tagtaccaga agatacggat
      961 cccagcctcg tggaaggcct gggccagttc ggcggtgaca tcgcgaccga agggcgtgcg
     1021 cgtgacctta tagtcggtca gcttgctgtc gaacatgcag aagccatcgt ggtgctttgt
     1081 gaggaagatg acgtagcgcg cgccggcgtc tttgacgagc ttcgcccact cgcgcgcgtt
     1141 gaacttcgtg gggttgaacc gcttgtagag gttgtcatac acctccaggg gcacctcgcc
     1201 ggtgccacca acacccggcc gggggccccc gcgcgaccaa ccgatctcgc cctcggcgat
     1261 gctgcacggc ccccagcaga tgaacacgcc gaacttggcc tcgcggaacc atttggtccg
     1321 tgcgtcgcgc tgctcggggg tctcgcccgg cgggccaccc ccctgggcga gcagaggagt
     1381 ggcgaacgtg aggcacagca ggctaagcgt caggcggttc atcggtgacc tccggtctct
     1441 cgggcgatct tcccgtcgcc gttacggggc cggtggagat aaccgctctc tgtgcgggct
     1501 ctcgagccct gccctgtctc ggatccggtc ggcggccggg tcgccggcgg cctcgtcgcc
     1561 gaaccagttc gcctcgaacg tgtgccagtc ggccgcgcac tggttgaagc ggatcggctc
     1621 ggccgacgtg gcgtggatca ggttgtcgac gatggtgaac cccttggagc cctgatcgaa
     1681 gaagatgccg ttgttcggcg ccccctgggc cgacccggcg cggagcgcat cgtgcagcca
     1741 gttcccccgg agcaccgtgc ccggctgcaa gccgagcgtg tagatgcagc ccccatcgca
     1801 gaggcgctgc agcacgtggt ggacgtggtt gtactcgatc cggttgttcg cgcaacaggt
     1861 gggcgactcg tcccactgga agccgaccga gatgcccgtg tagggcaggt tgtagaccag
     1921 gttgtgcgcc acgcgggtgc cgttggtgaa cgcgacccag atgccgacgg ctccgtagtc
     1981 gtcgagtccg cagtcgtgta caacgttgtt ctcgacgcgg ttgccccgtg gcaccacagg
     2041 atcggccagg ttcccattct cgcccaccat gatgccgctc gcaccgatcg agtggaccgc
     2101 gttgccgatg atgcggcaat gggtgcagcc cttcagcacc cggatcccga caccacgcag
     2161 gaactcgagc g
//
LOCUS       DAOSPL010000868         2170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_50600_length_2170_cov_2.633097, whole genome shotgun sequence.
ACCESSION   DAOSPL010000868 DAOSPL010000000
VERSION     DAOSPL010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50600_length_2170_cov_2.633097"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1833
                     /locus_tag="PLQ54_14640"
     CDS             <1..1833
                     /locus_tag="PLQ54_14640"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOS94556.1"
                     /translation="ADAAWLAATAERPLAGPGIEVVEQTWGTFRLNRSVWEAPLKLAG
                     RTYTRGLGTHAASTIRVHLPAPGRRFLALAGVDDNGNTRGAQPHVTFAVTADDRELLR
                     SGPAGVDDPPLDLNLDLAGARVLTLKIGEAEDGPNSDQADWCEARVELEDGRVVYLDE
                     LPILRGPTTPTTALPFSFTLDGKPSADLLPGWERSIQAVHNLNHRVSFRDPAGGLRIT
                     AHVRRFPDFPAVEWVLEISNEGTEESPLLERIRPLDLSLTAPPGEFVRLHHPRGSTCE
                     ITDFLPQVDELKTEGRIHLAPAGGRSSNGTLPFFHLDWGMGGMAAAVGWSGQWDANLA
                     RPDARTVRLDMGQETCHLRLKPGETIRTPRILLQWWNGGGESRGANMLRQLLMAHYCP
                     RVNGELIQPPVTHPTAWSILSSGVTGNETNQLEMLNAAADLGCEVYWLDAYWFPSGFP
                     GGVGTWIPRPEDFPRGLKPLGDAAHARGMQFCLWFEPERVARASQIAREHPELCIDLG
                     QGDLLYDLGNPEALAFMTDLLSKAISDYGVDIYREDFNIDPLPFWQRADAPDRQGITE
                     IRFVEGLYALWDSLVARHPGLIIDNCASGGRRIDLETVSRSYAL"
     gene            1858..>2170
                     /locus_tag="PLQ54_14645"
     CDS             1858..>2170
                     /locus_tag="PLQ54_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94557.1"
                     /translation="MLSLPTPLGTSAIASQVQNVGLGQYVAFSSNGLYSVDPYSVRSA
                     MCGGINMYFDLRKDDYDRDEARAAVAEVKELRPYFLGDLYPLTPVGVSPSDWAAYQLH
                     RE"
BASE COUNT          338 a          808 c          696 g          328 t
ORIGIN      
        1 gccgatgcgg cctggctcgc cgccacggct gagcgacccc tggcgggtcc cggcatcgag
       61 gtcgtcgagc agacctgggg gacattccgc ctcaaccggt cggtgtggga agccccgctc
      121 aagctggcgg ggaggaccta cacccgcggg ctgggcaccc acgccgcgag caccatccgc
      181 gtccacctgc ccgctcccgg gcgccgcttc ctggcactgg ccggggtgga cgacaacggc
      241 aacacgcgcg gcgcgcagcc gcacgtcacc ttcgccgtca cggccgatga ccgcgagctg
      301 ctcaggtcgg gtcccgccgg cgttgacgat ccgcccctgg acctcaacct cgacctcgcc
      361 ggggcgcgcg tcttgacact gaagatcggc gaggccgagg acgggcccaa cagcgaccag
      421 gccgactggt gcgaggcgcg cgtcgagctc gaggacggcc gggtggtata cctggacgaa
      481 ctgcccatcc tccgcggccc cacgacaccg accaccgcgc tgccgttctc gttcaccctc
      541 gacggcaagc cgtccgccga tctgctgccg ggctgggaac gctcaattca ggccgtccac
      601 aacctcaacc accgcgtcag tttccgcgac ccggcgggcg ggctccgcat cacggcgcac
      661 gtgcgccgct ttcccgactt ccccgccgtc gaatgggtgc tcgagatcag caacgagggg
      721 accgaggaat cgccgctcct cgagcgcatc cggccgctcg acctctctct gactgcaccg
      781 ccgggcgagt tcgtccggct gcaccacccg cgcggcagca cgtgcgagat caccgacttc
      841 ctcccgcagg tcgacgagct gaagactgag ggccggatcc acctggcacc cgcaggcggg
      901 cgctcttcga acgggacgct acccttcttc cacctcgact ggggcatggg cggcatggcg
      961 gcggccgtcg gctggtcggg ccagtgggat gccaacctgg cgcggcccga cgcgcgcacc
     1021 gtccggctcg acatggggca ggagacctgc cacctgcgcc tgaagcccgg tgagaccatc
     1081 cggacgcccc gcatcttgct ccagtggtgg aacggcgggg gcgagtcgcg cggggcgaac
     1141 atgctccgcc agctcctgat ggcccactac tgcccgcgcg tgaatggtga gctcatccag
     1201 cccccggtca cccatccgac ggcctggtcc atcctctcca gcggcgtgac cggcaacgag
     1261 accaaccagc tcgagatgct caacgccgcg gcggacctcg ggtgcgaggt ctactggctc
     1321 gatgcctact ggttccccag cggctttccc ggtggtgtgg gcacgtggat cccgcggccc
     1381 gaggacttcc cgcgtggcct caagccgctc ggcgatgccg cccacgcccg gggcatgcag
     1441 ttctgcctgt ggttcgagcc cgagcgcgtg gcccgcgcca gccagatcgc ccgggagcac
     1501 cccgagctct gcatcgacct cggccagggc gacctgctgt atgacctcgg caaccccgaa
     1561 gccctggcct tcatgaccga cctgctctcg aaggccatca gcgactacgg ggtcgacatc
     1621 taccgcgagg acttcaacat cgacccgctg cccttttggc agagggccga cgcgcccgac
     1681 cgccagggga tcactgagat ccgcttcgtg gagggtcttt acgccctgtg ggatagcctt
     1741 gtggcgcgcc accccgggct catcatcgac aactgcgcca gtggcggccg ccgcatcgac
     1801 ctcgagaccg tcagccgctc ttatgcccta tagcgcagcg atttccagga catcggcgtg
     1861 ctgagcctgc caaccccgct cggcacgtcc gccatcgcca gccaggtgca gaatgtcggc
     1921 ctggggcagt acgtggcatt cagctcgaac ggcctataca gtgtcgaccc ttacagcgtt
     1981 cgctcggcca tgtgcggggg catcaacatg tacttcgacc tgcggaagga cgactacgac
     2041 cgcgacgaag cccgagcggc tgtcgccgag gtgaaggagc tccggcccta cttcctgggc
     2101 gatctatacc cactgactcc ggtcggcgtg tcgccatcag actgggccgc ctatcagctc
     2161 catcgcgagg
//
LOCUS       DAOSPL010000869         2169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_50635_length_2169_cov_2.505676, whole genome shotgun sequence.
ACCESSION   DAOSPL010000869 DAOSPL010000000
VERSION     DAOSPL010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50635_length_2169_cov_2.505676"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="PLQ54_14650"
     CDS             complement(<1..1255)
                     /locus_tag="PLQ54_14650"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOS94558.1"
                     /translation="MLGGLLERAVPAVGWLRGYRRADLAGDLTAGVTVAVMLVPQATA
                     YSMLAGLPPQTGLYAATLPLAVYALFGSSRQLAVGPVAMVSLLVAAGCRPLAEAGSER
                     YIALAALLALVVGALQLAMGLLRLGVLVNFVSHAVISGFTSAAAIIIGLSQAKHLLGV
                     DVDGGESFPRLVRALVAAIPKTHGDTLILGLACIAALVILSKAAPRIPAALAVCAASI
                     LAAWLLGLDGRGVRMVGHVPQGLPPLSLPETEWAVMIDLLPTALTISLVGFMESVAVV
                     KFIAARERQKVEPSQELIGLGLANAAAAVCGGYPVTGGLSRTAVNYRAGARTGLAGLI
                     SAALVLATIVFLTPVFRHLPKVALAAIVMVAVTSLFDAREPARLFRVRPRDGHVLVLT
                     FAATLGLGIEPGLLLGVGLSLAQFIW"
     gene            complement(1298..1858)
                     /locus_tag="PLQ54_14655"
     CDS             complement(1298..1858)
                     /locus_tag="PLQ54_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOS94559.1"
                     /translation="MLARLHANQPAQLLIGLLIGVGFGFLLQKGGATSYDVIVGQLLL
                     TDFTVLKLMLSAVVVGMIGVHLLTAAGLAQLHIKPGGWGSTVVGGLIFGVGFGLLGYC
                     PGTAAAAAGHGTMDALIGGIGGILVGSALFALAYPRLARGPLAAGKFAKLTLTEALGL
                     RRTEYAVVVMAAVLIGLLAWLESAGL"
     gene            1904..>2169
                     /locus_tag="PLQ54_14660"
     CDS             1904..>2169
                     /locus_tag="PLQ54_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94560.1"
                     /translation="MVMPPAKNAMIICNEQLPSALETAVEEIRRETVPALKKLSAGLD
                     TWTALGSNLITSRDRLDQLLVRVDSIATELQRGKGTAGRLLTDPS"
BASE COUNT          432 a          727 c          739 g          271 t
ORIGIN      
        1 gccagatgaa ctgcgccagc gagagtccca cgccgaggag cagtccgggc tcgatcccca
       61 gcccgagcgt ggcggcgaag gtgagcacca gcacgtggcc atcgcgtggg cgcacacgga
      121 agagcctggc cggctcacgg gcgtcgaaca gactggtcac ggcaaccata acgatggcag
      181 ccagcgcaac cttgggcagg tggcgaaaca cgggagtcag gaagacgatg gtggcgagga
      241 caagggcggc cgagatcagg cccgcgagac cggtgcgggc tccggcgcgg tagttgaccg
      301 ccgttcgcga gagcccaccg gtgacgggat acccgccgca cacggcggcg gcggcattgg
      361 caagacccag cccgatgagc tcctggctgg gctcgacctt ctgcctctcc ctggcagcga
      421 tgaacttgac gaccgcgacg gactccatga agcccacgag cgagatggtc agcgccgtgg
      481 gcaacaggtc gatcatgacg gcccattcgg tctcgggaag cgacaggggc ggcagacctt
      541 gaggcacatg acccaccatg cggacccctc gaccgtcgag ccccagtagc catgcggcga
      601 gaatggaggc cgcacagacg gcgagggcgg ccgggatcct cggggcggct ttgctcagga
      661 tgacgagagc ggcgatgcag gccagaccga ggatcagcgt gtctccgtgg gtcttgggta
      721 tggcggccac gagagccctc accaggcgcg ggaatgactc gcccccgtcg acatccacac
      781 cgagcaggtg cttggcctgg ctcagcccaa tgatgatagc ggcggccgaa gtgaacccgc
      841 tgatcaccgc gtgcgatacg aagttgacca gcacacccag gcgcagcaat cccatggcga
      901 gctggagcgc tccgaccacc agcgccagaa gggccgccag ggcgatgtag cgttccgaac
      961 ctgcctcggc caggggccga cacccggccg ccacgaggag cgagaccatg gccacggggc
     1021 ccacggccag ttgcctcgat gacccgaaca gcgcatagac ggcaagcggc agcgtggcgg
     1081 cgtacaggcc ggtttgtggc ggcagaccgg ccagcatcga gtaggccgtg gcctgaggaa
     1141 ccagcatcac ggcgaccgtg acgcccgccg tcaggtctcc ggcaaggtcg gcacgccggt
     1201 atccccgcag ccaccccacg gcgggcaccg cccgctcgag caggccgccc agcacgggcc
     1261 tccccccccc tgtatcagct ccggctctcg cggaggccta gaggccagcg gattcgagcc
     1321 atgcgaggag cccgatgagg actgcggcca tgacgacgac cgcgtactcg gtgcgcctga
     1381 ggcccagtgc ctcggtgagc gttagcttgg cgaacttgcc cgccgccaac ggtccccgag
     1441 caaggcgggg gtaggccaga gcgaacagcg ccgacccaac gagtatcccg ccgatcccgc
     1501 cgatcagggc atccatcgtc ccgtggccgg cggccgcggc ggccgttccc gggcagtagc
     1561 cgagaagccc gaagccgacc ccgaagatga gcccgcccac caccgtcgaa ccccagccgc
     1621 ccggcttgat gtgaagctgc gccagaccgg cagccgtgag taggtgtacg ccaatcatgc
     1681 cgacgaccac ggccgagagc atgagcttga gcacggtgaa gtccgtcagc aggagctggc
     1741 ccacgatcac gtcataggat gtggcgccac ccttctgcag caggaagcca aacccgacgc
     1801 cgatgagcag gccgatcagc agctgggcgg gctggttcgc atggagtctc gccaacatgg
     1861 tgccacctcc tatgagccga ggacatggaa gaggaacatc gccgtggtga tgccgccggc
     1921 gaagaatgcc atgatcatct gcaatgaaca gcttcccagc gcgctggaaa cggcggttga
     1981 ggaaatccgc cgcgagacag tgccggcgtt gaaaaagttg agtgccggtc tcgacacgtg
     2041 gaccgcgctc ggctcgaacc tgatcacgtc gagggaccgg ctggaccaac tcctcgttcg
     2101 tgtggacagc atcgccaccg aacttcagcg gggaaaaggc acggcgggca ggctcctgac
     2161 cgatccatc
//
LOCUS       DAOSPL010000870         2168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_50658_length_2168_cov_3.421675, whole genome shotgun sequence.
ACCESSION   DAOSPL010000870 DAOSPL010000000
VERSION     DAOSPL010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50658_length_2168_cov_3.421675"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..>2168
                     /locus_tag="PLQ54_14665"
     CDS             292..>2168
                     /locus_tag="PLQ54_14665"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOS94561.1"
                     /translation="MLLGVLAGPVWVQLPASPLVTPGDQAEMRRFVSAKLEGTPAEEP
                     PATGLTVRANNDPVQLNGRAGRAMNIAGREYTRGLYCHAVSDVLVRLPGPGARLLAMV
                     GVDSNENTRPGRGSVVFSVSTGGRKLFESPRLREGMAGLPVDVDLGGATEFSLEVADG
                     GDGISCDQADWADARVVLASGQEVWLGDLPIVGGRRGPYNTTPPFSFRLGDRDFEQVL
                     AESQIERGTRELDAGRTEHTITLRHEASGLEARCVAIEYRDFPTVEWTLHLRNAAEAN
                     TPMISDVRSIDAWFERDEGPEFTVHHHTGSLCIATDYEPFESVLGPGQSLTVATSGGR
                     SSNAAFPFFAIGGPAQGVLMAVGWPGQWSAHFARNAGLGLRVQCGQELTHFTLYPGEE
                     VRSPLMALQFYQGDLSRARNVWRRWMLQYGMPRPHGRTLVPQTAACSSHQFGEMIHAN
                     DENQKFFIDRYLDERIRLDYWWMDAGWYVNESGWPNTGTWEVDRARFPGGLRSITDHG
                     HSKGVRSIVWFEPERVTAGTWLWREHPEWLLGSDGGTRLLNLGNPEALDWLIHHVSAL
                     IDSEGIDLYRNDYNIDPLQYWRENDAPDRQGITEIRYVEGFLAFWDGLLQRHPHMLID
                     T"
BASE COUNT          364 a          735 c          704 g          365 t
ORIGIN      
        1 gcatcaccgg cgacatcgcc acggtcatcg cccagctgcc gtccgtgctc gaatcgctct
       61 caggcgttga catcgccaag ctacttcaga acgtcccagg actgtcgaag tccgccatgg
      121 aggcccccgc gggaaccgcc ccgccgcagg cctcctgatg ccccgacctg aacggatggt
      181 ctcacacccg gccctccggc accatggggg gcccgtgtgt gtcttggtga gccgtcggaa
      241 acccggcgcc ggcatagccc gttggccgat cggggagtga gtcggttgct gatgctgttg
      301 ggcgttctcg cggggcccgt ctgggtccag ctacccgcgt cacctctggt cacgcccgga
      361 gatcaggccg agatgcggcg gttcgtctcg gccaagctcg aggggactcc cgccgaggag
      421 ccgcccgcca ctggactcac cgtgcgggcg aacaacgatc ccgtgcagct caacggccgc
      481 gcgggcaggg ccatgaacat cgcaggccgg gagtacacgc ggggcctcta ctgccacgcc
      541 gtgagtgatg tgctcgttcg cctgcctggc ccgggtgcca ggctcctggc catggtcggc
      601 gtcgacagca acgagaacac ccggcccggc aggggcagcg tcgtgttctc ggtcagcacc
      661 ggcggccgga agctcttcga gtcgccacgg cttcgtgagg gcatggccgg cctgcccgtg
      721 gatgtggacc tcggaggcgc cactgagttc tcgctcgaag tggcggacgg gggcgatggc
      781 atctcgtgcg accaggccga ctgggcggac gctcgcgtgg tgctggcgag cggacaggaa
      841 gtctggctcg gtgatctgcc catcgtcggc ggccgtcggg ggccctacaa cacgacacca
      901 cctttctcct tccgcctggg cgatcgagac ttcgaacaag tgctggctga gtcccagatc
      961 gagcgtggca cgcgcgaact ggatgccggc cggaccgagc acacgatcac cctccgccac
     1021 gaggcctctg ggctcgaggc gcgatgcgtg gccatcgagt accgggactt ccccaccgtg
     1081 gagtggactc tgcacctgcg caatgctgct gaggcgaaca cacccatgat ctcggacgtg
     1141 cgctcgatcg atgcgtggtt cgagcgcgac gagggtcccg agttcacggt gcatcaccac
     1201 accggcagtc tatgcatcgc cacagactat gagcccttcg agtccgtgct cgggccgggc
     1261 cagtcactta cggtggcaac gtcgggcggg cgttcatcca acgccgcatt ccccttcttc
     1321 gccatcggcg gaccggccca gggggtgctc atggccgtgg gctggccggg gcagtggtcg
     1381 gcccactttg cccgaaacgc cggactaggt ctccgggtcc agtgtggcca ggagctcact
     1441 cacttcacac tctatcccgg cgaggaagtc cgttcgcccc tgatggcgct gcagttctac
     1501 caaggcgact tgtcccgagc ccgcaatgtc tggcgccgat ggatgctcca atacggtatg
     1561 cctcgccccc acggccggac gctggttccc cagactgcgg catgtagctc gcaccagttc
     1621 ggcgagatga tccatgccaa cgacgagaac cagaagttct tcatcgaccg ctacctggac
     1681 gagcgcatcc gcctcgatta ctggtggatg gacgccggat ggtacgtgaa cgagagcgga
     1741 tggccgaaca ccggcacctg ggaggtcgac cgcgcccgtt tccccggtgg cctaaggtcg
     1801 atcactgatc acggccactc caagggcgtc cggtccatcg tgtggttcga gcctgagcgt
     1861 gtcaccgcag gaacatggct ctggagagag cacccggagt ggctgctcgg ctccgatggc
     1921 ggaacgcgat tgctgaacct gggcaacccc gaggcgctcg actggctcat ccaccatgtg
     1981 agcgcgctca tcgactccga gggcatcgac ctctaccgca atgactacaa catcgacccg
     2041 ctgcaatact ggagggagaa cgacgccccg gatcgccagg gcatcaccga gatccggtat
     2101 gtcgagggct tcctggcctt ctgggacgga ctgctccaaa gacaccccca catgctgatc
     2161 gacacctg
//
LOCUS       DAOSPL010000871         2168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_50671_length_2168_cov_2.593942, whole genome shotgun sequence.
ACCESSION   DAOSPL010000871 DAOSPL010000000
VERSION     DAOSPL010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50671_length_2168_cov_2.593942"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..1420
                     /gene="rfbB"
                     /locus_tag="PLQ54_14670"
     CDS             332..1420
                     /gene="rfbB"
                     /locus_tag="PLQ54_14670"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009504425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOS94562.1"
                     /translation="MSGWRRRIAVTGGCGFIGGAYLRQMVPRHPEDLYVNIDALTYAA
                     SPERVAAVAHEGNYVFERVDIRDTKAVVECLGRHSITDVVHFAAESHNDRAILTPLRF
                     VETNVVGTANLLESWRVVAKGSGGRFHHISTDEVYGSLPIEGGEGFTESTPYDPSSPY
                     SASKAAADHLVRAWHRTFGLDVVITNCSNNFGPFQFPEKLLPVMISNALSGRRLPVYG
                     DGMNVRDWIHVEDHCDALDLVLRQGRAGRTYNIGADCEIPNITLVTMLCEIIDQREGP
                     LPGGRSRASLIEFVPDRPGHDRRYAIDASRIREELGWSPRHEFRQALEDTVDWYRTHR
                     AWVENCQKSDEFRAYAERNYGERSGDVS"
     gene            1438..>2168
                     /gene="rfbA"
                     /locus_tag="PLQ54_14675"
     CDS             1438..>2168
                     /gene="rfbA"
                     /locus_tag="PLQ54_14675"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOS94563.1"
                     /translation="MRKGIVLAGGAGTRLYPLTEVTSKQLLPVYDKPMVYYPLATLIS
                     GGISDILLISTPRDLPRFREVLGDGSRFGIALSYVEQPRPEGIAQSFILGRDFIADQA
                     VCLILGDNLFYGEMDPFFEALAHTEGATIFGYPVRDPQRYGVIELDGHGRPVSIEEKP
                     AHPRSSLAVPGLYVYDGRVCDFALELRPSARGELEITDLNRAYMAEGTLRVGRLGRGT
                     AWLDTGTPESLHAASTFVATIEQRQ"
BASE COUNT          341 a          721 c          745 g          361 t
ORIGIN      
        1 accattcggg agcggcggcc gagcgcttcg agcgcgccct gcgtgagatg ctcgagttcc
       61 tgcgcggcgg cgcggttccg cagaccgagt gccacgacaa catccagtct ctggccatgg
      121 tgttcggcgc catcgagagc tcgcggcgcg cggagcgcgt ttctatcgaa gagtgaccgc
      181 aggcctggcg ggtaactcgc ggcggtgccc aaccagctcg ccggcgagct tgacgtgagt
      241 tgggttaccg cggagggtat gaccctattg ggtggcgtga gggccgcggc cggggccggg
      301 cagcggccgg gtggcgcggg gaggtggtca tgtgtccggc tggcgtcggc gcatcgccgt
      361 gacgggcggc tgcggcttca tcggcggggc ctatctccgg cagatggtgc cgcgacaccc
      421 agaggatctt tacgtgaaca tcgacgcgct gacctacgcg gccagcccgg agcgcgtggc
      481 cgccgtggcc cacgaaggca actacgtctt cgagcgcgtc gacatccgtg acaccaaggc
      541 ggtcgtggag tgtctgggtc gtcattcgat caccgacgtg gtccacttcg cggcggagtc
      601 gcacaacgac cgggcgatcc tgactccgct acgattcgtt gagacgaacg tggtcggaac
      661 ggcgaacctg ctcgagtcgt ggcgcgtcgt ggccaagggc agcggcgggc gcttccatca
      721 catctccacc gacgaggtct acgggtctct gcccatcgag ggcggcgagg gcttcaccga
      781 gagcacgccc tacgatccca gctcgcccta ctcggcgagc aaggccgcgg cagaccacct
      841 ggtgcgggca tggcaccgga ctttcgggct cgatgtcgtc atcaccaact gctcgaacaa
      901 ctttgggccg ttccagttcc ccgagaaact cctgcccgtg atgatctcga atgccctctc
      961 gggccgccgg ctgccagtct acggcgacgg gatgaacgtg cgggactgga tccatgtcga
     1021 ggaccactgc gacgctctgg acctggtgct tcgccaaggc cgcgcgggcc ggacctacaa
     1081 catcggcgcg gattgcgaga tcccgaatat caccttggtg accatgctgt gcgagatcat
     1141 cgaccagcgc gagggtcccc ttcccggcgg caggtcccgt gcgagcctga tcgagttcgt
     1201 cccggatcgc ccggggcacg accgccgcta tgccatcgat gcgagccgga tccgtgagga
     1261 gctcggctgg tcgcctcgcc acgagttccg ccaggcgctc gaggacacgg tggactggta
     1321 ccggacccac cgggcgtggg tcgagaactg ccagaagtca gacgagttcc gcgcctacgc
     1381 cgagcgcaac tacggcgagc ggtcgggtga tgtgagttga tggagcgctc cggacgcgtg
     1441 cggaagggca tcgtgctggc cggtggagcg ggaacgcgcc tctacccgct cacggaggtc
     1501 acctcgaagc agctccttcc cgtctatgac aagcccatgg tctactaccc gctggcgacg
     1561 ctcatatcgg gcgggatcag tgatatactg ctgatcagca ctccacgcga cctgccgcgc
     1621 ttccgcgagg tgctcggcga cggctcgcgc tttggcatcg cgctgtcgta cgtggaacag
     1681 ccccggcccg agggcatcgc gcaatcattc atcctcggcc gggatttcat cgccgaccag
     1741 gcggtgtgcc tcatcctcgg cgataacctc ttctacggcg agatggaccc gttcttcgag
     1801 gcgctcgcgc acaccgaggg tgccacgatc tttggctacc ccgtgcgcga tccgcagcgg
     1861 tacggcgtca tcgagctgga cggccacggc cggccggtct cgatcgagga gaagccggcg
     1921 cacccgcgca gtagcctcgc ggttcccggt ttgtacgtgt acgacgggcg cgtctgtgac
     1981 ttcgcgctcg agctgaggcc ctcggcccgg ggggagctcg agatcaccga cctcaatcgt
     2041 gcctacatgg cggagggaac gctccgggtg ggccgcctcg gccgcggcac ggcctggctc
     2101 gacacgggca cgcccgagtc gctgcacgcc gcgtcgacct tcgtcgccac catcgagcag
     2161 cgccagag
//
LOCUS       DAOSPL010000872         2167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_50682_length_2167_cov_3.809659, whole genome shotgun sequence.
ACCESSION   DAOSPL010000872 DAOSPL010000000
VERSION     DAOSPL010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50682_length_2167_cov_3.809659"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="PLQ54_14680"
     CDS             <1..828
                     /locus_tag="PLQ54_14680"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOS94564.1"
                     /translation="ALGSAASGPPVGTRVAVEPLTVCGHCRGCIEGHYQLCDHLKLNG
                     HHYEGSFCEFTRAQSVFCFPIPDAMSFRVAAFAEPVAVAVHAVKRSRATVGDLVVVLG
                     AGPIGNLCMQVARAAGARVLISDVDPPKLEVARATGADFVVNAARESVEDAVRDLTGG
                     TGADIVMEAAGVSATLRQTVHLARKGGTIVAVGFTADETDSIRLTDVTIRELDFLGTV
                     IYCRDYPATLDLLSSGRVITEPLVTHEYTLEALAEQAAVVASKPSGMIKPLILPQQR"
     gene            849..1712
                     /locus_tag="PLQ54_14685"
     CDS             849..1712
                     /locus_tag="PLQ54_14685"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOS94565.1"
                     /translation="MRWICMACLAAVVSGAWGQAPSAAADADEGVRVGGVVLSPNDLL
                     ARLAERFAGPVLEERIIAVLVEQKAQAQGIDLPQGSVETAFRQSMERVPAEQREAFLA
                     ARGLTLAVALRQIRIDLLVNALIDQTIQVTDAELSERYEKRRAALRRPERRIVTHITC
                     DSEEAALRAYDDLKRDPLVDPQLTQIMEAPRGDLSEADRLTQAVFRQTDQSVRGVCHP
                     VQLPDGWHVLRVEQVVEEQDPTLAECRDLLISEIRAEKREQAKPAWFQALRDGASVQL
                     HIDLPGPQSPR"
     gene            1726..>2167
                     /locus_tag="PLQ54_14690"
     CDS             1726..>2167
                     /locus_tag="PLQ54_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94566.1"
                     /translation="MPVVLYTPTPEHTRNVFRPEVYAALGREFDLRENTLGRPMTVDD
                     VVQGIGDADALVTGWGTCALTAPVFDAAPRLRLIAHSAGSVRAILTPDLIERYVRPRG
                     IVVFSANRAIALNVAEATIGLLIMASRRWVEHIQHVRGGGWRTPE"
BASE COUNT          323 a          789 c          732 g          323 t
ORIGIN      
        1 gccctgggct ccgccgcatc gggcccgccc gtgggcaccc gggtcgccgt ggagccgctc
       61 accgtctgtg gacactgccg gggctgcatc gagggccact accagctctg cgaccacctc
      121 aagctcaacg gccatcacta tgagggcagc ttctgcgagt tcacacgagc ccagtcggtg
      181 ttctgcttcc cgatcccgga cgccatgagc ttccgcgtcg cggccttcgc cgaacccgtg
      241 gcggtggcgg tccatgcggt gaagcgctcg cgggccacgg tgggcgacct cgtggtggtc
      301 ctcggtgccg ggccgatcgg caatctgtgc atgcaggtcg cccgggcggc cggggcccgc
      361 gtcctgatct ccgacgtgga tccacccaag ctcgaggtcg ctcgtgccac gggcgccgac
      421 tttgtggtga atgcggcccg ggagtccgtc gaggatgcgg tgcgggatct caccggcggg
      481 accggtgccg atatcgtcat ggaggccgcg ggcgtctccg ccaccctgag acagacggtc
      541 cacctcgctc gcaagggggg caccatcgtc gccgtgggct tcaccgcgga cgagacggac
      601 agcatccggc tcacggacgt gaccatccgc gagctggact tcctcggcac ggtgatctac
      661 tgccgggact accccgccac cctggatctg ctgagctcgg gccgcgtgat caccgagccg
      721 ctggtgacac acgagtacac gctcgaggcc ctggcggaac aagcggccgt ggtcgccagc
      781 aaaccgagtg gcatgatcaa accgctgata ctgccccagc agcggtagtc tgaggggagc
      841 gccggaccat gcgatggatc tgcatggcgt gtcttgccgc ggtggtcagc ggcgcctggg
      901 gccaggcgcc gtcggccgcc gccgatgccg atgagggggt gcgcgtcggc ggcgtcgtcc
      961 tgtctcccaa cgacctgctc gcccgcctgg cggagcgctt cgccggcccg gtgctcgagg
     1021 agcggatcat cgccgtcctg gtcgagcaga aagcccaggc ccaggggatc gacctccccc
     1081 agggctcggt cgagacggcc ttccggcagt ccatggagcg ggttccggcc gagcagcgcg
     1141 aggccttcct ggccgcccgc gggctgaccc tggccgtggc actccgccag atccgcatcg
     1201 acctgctcgt caacgcgctg atcgaccaga ccatccaggt caccgacgcc gagctcagcg
     1261 agcgctacga gaagcggcgg gccgcgctcc ggcgccccga gcgccgcatc gtcacccaca
     1321 tcacctgtga cagcgaagag gcggccctgc gcgcgtacga tgacctcaaa cgcgacccgc
     1381 tcgtcgaccc gcagctcacg cagatcatgg aggccccccg gggcgacctg tcggaggccg
     1441 atcggctcac ccaggccgtg ttccgccaga ccgaccagag cgttcgcggc gtctgtcacc
     1501 cggtccaact gcccgatggc tggcatgtgc tgcgcgtcga gcaagtggtc gaggaacagg
     1561 atccgacgct ggccgagtgc cgtgacctcc tgatcagcga gatccgcgcc gagaaacgtg
     1621 agcaggcgaa gcccgcgtgg ttccaggcgc tgcgcgacgg cgcgtccgtc cagttgcaca
     1681 tcgacctccc cggaccgcaa tccccgaggt agcgaggcga tccccatgcc cgtcgtgctc
     1741 tacacgccca cgcccgagca cacccgcaac gtgttccggc ccgaggtcta tgcggccctc
     1801 gggcgcgagt tcgacctccg cgagaacacc ctcggccggc cgatgacggt ggacgacgtc
     1861 gttcagggca tcggcgatgc cgacgccctg gtcaccggtt ggggcacctg tgccctcacg
     1921 gccccggtgt tcgatgcggc cccccggctg cgcctcatcg cccactcggc cgggagcgtc
     1981 cgtgccattc tcacacccga tctcatcgag cgctacgtgc gcccccgggg tatcgtggtg
     2041 ttcagcgcca accgggccat cgccctgaat gtcgccgaag cgaccatcgg actcctgatc
     2101 atggcgagcc ggcgttgggt cgagcacatc cagcacgtgc gcgggggcgg ctggcggacc
     2161 cccgaga
//
LOCUS       DAOSPL010000873         2166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_50723_length_2166_cov_3.242539, whole genome shotgun sequence.
ACCESSION   DAOSPL010000873 DAOSPL010000000
VERSION     DAOSPL010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50723_length_2166_cov_3.242539"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PLQ54_14695"
     CDS             <1..669
                     /locus_tag="PLQ54_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOS94567.1"
                     /translation="RVGAHDDLAQGQSTFMVEMTETASILNSATRDSLIILDEIGRGT
                     STFDGLAIAWAVAEYIHNVVGAKTLFATHYHQLNALCQSLPRVRNLRVLVKEEGERMT
                     FLHRIAEGGTDRSYGVQVARLAGLPRAVIERAREVLWSLEQENSVGKLPAPKATVPKL
                     DFSMSQLSLFGPPEPDPRLELLRSVERVDVNGLTPLAALNFLADLRQKLIATAQDGSG
                     EVRS"
     gene            666..1055
                     /locus_tag="PLQ54_14700"
     CDS             666..1055
                     /locus_tag="PLQ54_14700"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOS94568.1"
                     /translation="MSRSPQQEALDALLAEADRMVPSITARGVMALAAEMPELLVIDV
                     RDRDEYGSCHIRRSIHISRGQLEFHIEDVAPELSHPMLLVDRAGARSLLAARALQAMG
                     YTHVFVLQGGLDAWLQEGLPVSSGWTV"
     gene            1184..2062
                     /locus_tag="PLQ54_14705"
     CDS             1184..2062
                     /locus_tag="PLQ54_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HOS94569.1"
                     /translation="MGSSTRDFRVEAEFLGQTVLVERIGCDGDLRRFSGMFTELDGKA
                     DALGIGGADLYVWASGRRYMFRDVARAVAGARTTPVLDGSGLKNSIERETVRWLQRQG
                     LVDFPRARTLLVSTVDRFGLGEELSAMGGPVLFGDVMFCLHIPIPIRRLKTLQITARL
                     LLPVITQMPFRWLYPTGSSQERIEPRFTRHYQWADVIAGDFHYIKRHMPDRLEGKTIL
                     TNTIRESDVALLRERGARRLITSTPEISGRSPGTNVFEALIVALSGGRPDELSQDDYM
                     AHVRQMGWQPRVVELQ"
BASE COUNT          363 a          731 c          687 g          385 t
ORIGIN      
        1 cgcgtcggcg cccatgatga cttggctcag gggcagtcga ccttcatggt cgagatgacc
       61 gagaccgcca gcatcctgaa cagcgcaacg cgcgacagcc tcatcatcct cgacgagatc
      121 ggccgcggca ccagcacctt cgacggcctc gccatcgcct gggccgtggc cgagtacatc
      181 cacaacgtgg tgggtgccaa aacgttgttc gcgacccatt accaccaact caatgctctc
      241 tgccagtcgt tgccgcgcgt gcggaacctt cgggtcctgg tcaaggagga gggcgagcgc
      301 atgaccttcc tccatcgcat cgccgagggc ggtaccgacc gcagctacgg ggtgcaggtg
      361 gcccgactcg ccggcctgcc ccgcgctgtg atcgaacgcg cccgcgaggt gctctggagc
      421 ctggaacagg agaactcggt gggcaaactg cccgcgccca aggccacggt tccgaagctc
      481 gatttctcca tgagccagct ctcgctgttt ggtccgcccg agcccgatcc gcgtcttgag
      541 ttgctccgta gcgtcgaacg cgtcgacgtg aacggactca caccgctcgc cgcgctcaac
      601 ttcctcgccg acctgcgcca gaagcttatc gccaccgccc aggacggctc gggggaggtc
      661 aggagttgag ccgctcgccc cagcaagagg cattggacgc cctgttggcc gaggcggatc
      721 ggatggttcc ctcgatcacc gcacggggtg tcatggcgct ggcggccgag atgcccgagt
      781 tgttggtgat cgatgtgcgg gatcgcgatg agtatggatc gtgccacatc cgacggtcga
      841 tccacatctc acgcggccag ctcgagtttc acatcgagga cgtggccccc gagctctcgc
      901 accccatgct gctcgtcgat cgcgccgggg ctcgaagcct gctggccgcg cgcgccttgc
      961 aggcgatggg ctacacccac gtctttgttc tccagggcgg gctcgacgca tggcttcaag
     1021 aaggactgcc cgtctcctcc ggctggaccg tgtgagcggc ccgtgccagc aaggaccttg
     1081 ttccgccgga cgggaaacta ctccgctgcg gagcggaggc gcgaccgctc atgggtcatg
     1141 cacagggagg cgtgccgcca tcaagcgcgt tgtcagcatc agcttgggat cctccacacg
     1201 ggacttccgt gttgaagcgg agttcctcgg gcagaccgtg cttgtcgagc ggatcgggtg
     1261 cgatggtgat ctgcgccggt tttccggcat gttcaccgaa ctcgacggga aggccgacgc
     1321 cttggggatc ggcggcgcgg atctctacgt ctgggcttcc ggccggcggt acatgttccg
     1381 cgacgtggct cgagcagtcg ccggggcacg caccactccg gtgctggatg gcagtggcct
     1441 gaagaacagc atcgaacgag agaccgtgcg gtggctccag cggcagggcc tggtcgattt
     1501 cccgcgggcg aggacactgc tggtctcgac ggtcgaccgt ttcggactgg gcgaggagct
     1561 ttcggccatg ggcgggccag tcctcttcgg cgatgtcatg ttctgcctgc acatcccgat
     1621 cccgatccgt cgcctgaaga ccctgcagat caccgcccgc ctcctccttc ccgtgattac
     1681 ccagatgccg ttcaggtggc tatacccgac cgggagctcc caggaacgta tcgagccacg
     1741 cttcacgcgt cactatcagt gggccgatgt cattgcgggg gatttccact acatcaagcg
     1801 gcacatgccc gaccgcctcg aaggtaagac tattctcacc aacaccatcc gcgagtccga
     1861 tgtcgccctg ctccgcgagc gcggtgcccg gcgtctcatc acctccacac cggagatcag
     1921 cggaagatca ccagggacca acgtcttcga ggccctcatc gtggcgctct ccggcgggcg
     1981 acccgatgag ctctcgcaag acgactacat ggcacacgtc cgccagatgg gctggcaacc
     2041 cagggtcgtc gagctccagt agcgctctgc ctgggaggca ctcgcatgcg aaccagtact
     2101 gccgtcgcgc tcctgatcct cgtcggagcg cttggggtcg cacacagtca ggagccgggc
     2161 ccgaac
//
LOCUS       DAOSPL010000874         2166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_50728_length_2166_cov_2.920417, whole genome shotgun sequence.
ACCESSION   DAOSPL010000874 DAOSPL010000000
VERSION     DAOSPL010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50728_length_2166_cov_2.920417"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="PLQ54_14710"
     CDS             complement(<1..867)
                     /locus_tag="PLQ54_14710"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOS94570.1"
                     /translation="MIAPERRAVAMEPQLYAVFATFSGLLGLCVGSFLNVAICRLPED
                     ESLGGRSHCTSCGKEILARDLIPVLSQLLLRSRCRFCGARFSYRYMAIELITGLVFLG
                     LFFYCGPGRPVLLVALWICFASLIALFVIDWQTTLIPHPLWIAILVAGVGYDIYGLAV
                     GAHEMMVLFPVTTAGGQFAVALPRSVIGALVGLIGLRVFVFLADLAFRKETMGFGDIY
                     LTGAAGAFLGPGIELVLYIVIAAFAGALIGGTWMLWHRKEREDTVIPFGPFLAVSLAV
                     MILWGDVIANAVR"
     gene            complement(959..>2166)
                     /locus_tag="PLQ54_14715"
     CDS             complement(959..>2166)
                     /locus_tag="PLQ54_14715"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOS94571.1"
                     /translation="GAEFTGTIEADSEQALRTNLVDQGYVVSSIQKTKGTGVVKGRGR
                     GMSRFKRVKLNDLAQFCRQFATMINAGVTVVRCLSVLQEQTTNPNLRAMIADIRAEVE
                     GGNSLSGAMAKYSRTFSNLAIGLVRAGEVGGVLDETLNKLAVFLEKDLELRRKVKSAM
                     TYPTIVMIAAVGIVLFLMTFILPKFMALFDELGIGRDKMPKMTLILRDASDFLVHYWY
                     YALAGGIIIFIALKFLGRTKVGRRYIDLVKLKLPVFGKLIHKVALSRFASTLSTLLSS
                     GVPILQAMETVAGTVGNEIIAEAVLDARASVREGEEIGTPLRNSGMFPPMVVQMISIG
                     EETGSLDAMLAKVSEFYESEVDTALASLTAAIEPMLIVFLGGVVGFIVVAMFMPLVEI
                     INSLSSQNE"
BASE COUNT          451 a          648 c          734 g          333 t
ORIGIN      
        1 tctgacggcg ttcgcgatga cgtcacccca aaggatcatc acggccagag agacggccag
       61 gaatggcccg aatgggatga cggtgtcctc tcgctccttg cggtgccaga gcatccaggt
      121 gccaccgatg agggccccgg caaaggcggc gatgacgatg tagaggacga gctcgatgcc
      181 gggaccgagg aacgcccccg cagcaccagt gagatagata tcaccaaagc ccatggtctc
      241 cttgcggaat gccaggtcgg ccaggaacac gaacacgcgc aacccgatca gcccgaccag
      301 ggcgccgatc accgaacgag ggagggccac agcgaactgg ccgccggcgg tggtgacagg
      361 gaacagcacc atcatctcgt gtgccccgac ggccaggccg tagatgtcgt agccgactcc
      421 ggcgacgagg atggcaatcc aaagtggatg agggatgagg gtggtctgcc agtcgattac
      481 gaataaggcg atgagcgagg cgaagcagat ccacagcgca acgagcagca ccgggcggcc
      541 gggcccacag tagaagaaca ggcccagaaa gacgaggccg gtgatcagct cgatcgccat
      601 gtaccggtag ctgaaccggg ccccacagaa gcggcagcgg ctgcggagga gtagctggct
      661 gaggacgggg atgaggtcgc gcgccagtat ctccttgcca caggacgtgc agtgggagcg
      721 cccacccagg ctctcgtcct cgggcaggcg gcagatggcg acattcagga agctcccgac
      781 gcacaggccc aggaggccag agaaggtggc gaagacagcg tagagctggg gttccatggc
      841 cacggcgcgc cgttcgggcg cgatcacccc actccctggc gcatacaaca cggggcgagg
      901 aggtcctcgc cccgtgtcgc tgcgttctca gacgccggcg agccgccggg ccgcccgcct
      961 actcgttctg ggaagacagg ctgttgatga tctcaaccag cggcatgaac atggcgacga
     1021 cgatgaatcc aacgacgcca ccgaggaaaa cgatcaacat cggctcgatg gcggccgtca
     1081 gggaagccag ggccgtgtcg acctcggact cgtagaactc cgacacctta gccagcatgg
     1141 cgtcgagcga gccggtctcc tcgccgatgg agatcatctg aaccaccatg ggcgggaaca
     1201 tgccggagtt acgcaggggc gtgccgatct cctcgccctc gcggacgctg gcgcgcgcgt
     1261 cgagcacggc ctcggcgatg atctcgttgc cgacagtgcc ggccacggtc tccatggcct
     1321 ggaggatggg cacaccactg ctgagcaggg tggagagcgt ggaggcgaag cgcgatagtg
     1381 caactttgtg gatcagcttg ccaaagacgg gcagcttgag cttaaccagg tcgatgtagc
     1441 ggcgcccaac cttggttcgg ccgagaaact tgagggcgat gaagatgatg atgcccccag
     1501 ccagggcgta gtaccagtag tggacgagga agtcgctggc atcgcggagg atgagggtca
     1561 tcttcggcat cttgtcgcgg ccgatgccga gttcgtcgaa cagggccatg aacttgggca
     1621 gaatgaaggt catgaggaag agcacgatgc cgaccgccgc gatcataacg atcgtggggt
     1681 aggtcatggc ggacttgacc ttccggcgaa gttcgaggtc cttttcgagg aagacggcga
     1741 gcttgttgag cgtctcgtcg agcacgccgc cgacctcgcc cgcgcggacc agaccgatgg
     1801 ccaggttcga gaaggttcga gagtacttgg ccatggcccc ggacagcgag ttacccccct
     1861 cgacctcagc gcgaatgtcg gcgatcatgg cccttaggtt ggggttggtg gtctgctcct
     1921 gaagaaccga gaggcagcga accacggtca cgccggcgtt gatcatggtg gcgaactgcc
     1981 ggcagaactg ggccaagtcg ttgagcttga cgcgcttgaa gcgcgacatg ccacgtcccc
     2041 ggcctttgac cacgccggtg cccttggtct tctggatcga cgagaccaca tagccctggt
     2101 cgaccaggtt agtgcgcaac gcttgctcgc tgtcggcctc gatggttccc gtgaactcgg
     2161 caccgc
//
LOCUS       DAOSPL010000875         2165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_50760_length_2165_cov_3.601422, whole genome shotgun sequence.
ACCESSION   DAOSPL010000875 DAOSPL010000000
VERSION     DAOSPL010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50760_length_2165_cov_3.601422"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PLQ54_14720"
     CDS             complement(<1..809)
                     /locus_tag="PLQ54_14720"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOS94572.1"
                     /translation="MKALTTPFYQVLVRHADIRDDLAVGETVPAWSYASPDDPRLAEL
                     RERYGLARVIEGRAELDAIRALLDWAYRIVEHDGGAHNPAPRDALNILRTCETTGARV
                     NCRMKAIVLCEALLAVGFRARYVTCLPAVSDGDCHVITLAYAASVGKWVSVDPTFNTY
                     FSAEDGTMLNVVEARRAYRSGQVPRHRRIDLEARGPLCCGGIECASYDEFYDLYMCKN
                     SFRFACTLASEPGIDSHEAPATVVLAPPDDPWVAEGGPAEWRRPVVVTHSKA"
     gene            complement(870..1193)
                     /locus_tag="PLQ54_14725"
     CDS             complement(870..1193)
                     /locus_tag="PLQ54_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HOS94573.1"
                     /translation="MQRYGMVLKLKPGKLDEYTRLHAAVWPDVLKMIAECNIRNYSIF
                     HKDGWLFSYFEYHGDDFEADMAKMAADPTTQRWWDVCKPCQEPLETRAPGEWWANMDE
                     VFHTD"
     gene            complement(1209..1943)
                     /locus_tag="PLQ54_14730"
     CDS             complement(1209..1943)
                     /locus_tag="PLQ54_14730"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS94574.1"
                     /translation="MEARLGDALARDIPAWTDRVNSYADDFAVFGGLCEPYEYLWRCV
                     GTENALMWMAQEPELLEAFVDRIGRFMLELGRAQIAAGAGRLSGMYIWGDVAYRGGML
                     FSPAVWRRLFRPHVKALIHLCHSHGLPVIYHGCGDARAILDDLVEDGLDAYNPLEAKA
                     GLDVVELKPRYAGRLAFVGNIDMRVLEAGEPTAIRAEVARKLQAARGGGYVLQSDHSV
                     TSQVAPESYELALATLRDLGTFPLSV"
     gene            complement(1916..2165)
                     /gene="lysK"
                     /locus_tag="PLQ54_14735"
                     /pseudo
     CDS             complement(1916..2165)
                     /gene="lysK"
                     /locus_tag="PLQ54_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504494.1"
                     /note="class I; LysRS1; catalyzes a two-step reaction,
                     first charging a lysine molecule by linking its carboxyl
                     group to the alpha-phosphate of ATP, followed by transfer
                     of the aminoacyl-adenylate to its tRNA; in Methanosarcina
                     barkeri this enzyme charges both tRNA molecules for lysine
                     that exist in this organism (but the tRNALysUUU very
                     poorly) and in the presence of LysRS2 can charge tRNAPyl
                     with lysine; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="lysine--tRNA ligase"
BASE COUNT          366 a          733 c          714 g          352 t
ORIGIN      
        1 gccttgctgt gggtgaccac aacgggccga cgccactcgg ccgggccgcc ctcagcaacc
       61 cacggatcgt ccggtggggc caggacgacg gttgccgggg cctcgtgcga atcgatgccg
      121 ggctcgctgg cgagcgtgca cgcgaaccgg aacgagttct tgcacatgta gaggtcatag
      181 aactcgtcgt agctcgcgca ctcgatcccg ccgcagcaga gcgggcccct ggcctccagg
      241 tcgatgcgcc ggtgccgggg aacctgaccc gagcggtagg cccgccgcgc ctcaaccacg
      301 ttgagcatcg tcccgtcttc ggcggagaag taggtgttga acgtcgggtc aacggagacc
      361 cacttcccga ccgacgccgc gtatgccaga gtgatcacgt ggcaatcgcc gtcgctcact
      421 gccggcaagc acgtgacgta gcgtgccctg aagcctacgg ccaggagagc ctcacacagg
      481 acgatggcct tcatccggca gttgacccgc gccccggtcg tctcgcaggt gcgcaggatg
      541 ttcagcgcat cccgtggggc ggggttgtgc gcgccgccat cgtgctccac gatccggtag
      601 gcccagtcca ggagggcccg tatcgcgtcg agctccgctc tgccctcgat aacccgggcg
      661 aggccgtacc gctcccggag ttccgccaga cgcggatcat cgggggaggc gtacgaccat
      721 gcagggacag tctcgccgac ggcgaggtca tcgcggatgt cggcatggcg caccagtacc
      781 tggtagaagg gtgtcgtcag ggctttcact ccggccgcct aaccattcgc ggcggcagtc
      841 gctggccgcc cgcccgtttc ggggcgacgt cagtccgtgt ggaagacctc gtccatgttc
      901 gcccaccact caccaggggc tcgcgtctcg aggggctcct ggcatggttt gcacacatcc
      961 caccatcgct gggtcgtcgg gtcagcggcc atcttggcca tgtcagcctc gaagtcatcg
     1021 ccatggtact cgaagtagct gaacagccag ccgtccttat ggaagatgga gtagttgcga
     1081 atgttgcact cggcgatcat cttgagcaca tccggccaca ccgccgcgtg gagccgggtg
     1141 tattcgtcga gcttgccggg tttgagcttc agcaccatgc cgtaccgctg catacggatg
     1201 cctcctcgct agaccgagag cgggaacgta ccaagatccc tgagcgtcgc cagcgccagt
     1261 tcgtagctct ccggggctac ctggctggtc accgagtgat ccgactgaag cacatagccg
     1321 ccgccacgag ccgcctggag cttgcgagct acctcggcgc ggatggccgt cggctcaccg
     1381 gcctcgagca cgcgcatgtc gatgttcccg acgaatgcca gcctgccagc atagcggggc
     1441 ttgagctcga cgacgtcgag ccccgccttg gcctccagcg gattgtaggc atccaggccg
     1501 tcctcgacca ggtcatcgag gatggcgcgg gcgtcgccac agccgtggta gatgacgggg
     1561 aggccgtgcg aatggcacag gtggatgagg gccttcacgt gcggccggaa caggcggcgc
     1621 cacacggcgg gagagaacag catgccgccg cggtaggcga catcgcccca gatgtacatc
     1681 cccgagaggc ggccggcgcc cgccgcgatc tgggcccgcc cgagttcgag catgaagcgg
     1741 ccgatgcggt ccacgaaggc ctcgagcagc tccggctcct gagccatcca catcagtgcg
     1801 ttctccgttc ccacgcaacg ccagaggtac tcatagggct cgcagaggcc accgaacacg
     1861 gcgaaatcgt cggcgtagga gttcacgcgg tcggtccacg ccgggatgtc gcgtgccagc
     1921 gcatcgccca atcgggcttc cattgcagct tgcaatgtcc gccggtgacc ggaacggtca
     1981 cgcgttcgtt cgtttccgga tcgtcgtagg agatcgtgcc cgcgctcacg tcgcgcgcga
     2041 cgatcggcac ctgcaggacg accccgctac gcgggcagac gggcaggaac ggcgaatagc
     2101 tcgccgcccg ctcctcgcgc aggctcggca gcatgatcgc catgacgcgg tcgtagcgct
     2161 ccagc
//
LOCUS       DAOSPL010000876         2158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_51024_length_2158_cov_2.589634, whole genome shotgun sequence.
ACCESSION   DAOSPL010000876 DAOSPL010000000
VERSION     DAOSPL010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51024_length_2158_cov_2.589634"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..2079
                     /gene="dxs"
                     /locus_tag="PLQ54_14740"
     CDS             190..2079
                     /gene="dxs"
                     /locus_tag="PLQ54_14740"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOS94575.1"
                     /translation="MSRLLDTIDSPRDLKRLSAADLPRLAQEIRSALIETVSMVGGHL
                     APNLGVVELTIALHRAFDAPRDRIVFDVGHQAYVHKLLTGRREQFGTLRRRGGISGFP
                     RRCESPYDVFGTGHGSTSISAALGIATARDLAGEHYQVVAVIGDGAMTGGMAFEGLNQ
                     VAERDTDLVIILNDNGMSISPNVGAMARYLQRFRLDPGYSSAKRSVEGLVRRYDRFGL
                     GNHVLDAVEKLKDGAKSLFLPGMLFEELGLRYFGPVDGHDLDELTRTFEQVRRLKGPR
                     IVHVVTQKGRGYRLAEEDAESYHGCKPFDVDQGQLDPPVPQKTYSEVFSEALAGLAEA
                     DSRIVAVTAAMCEGTGLKPFARRFPHRFFDVGMAEEHAVTFAAGLAAEGWRPVVAIYS
                     TFLQRAYDQVLHDVCLQGLPVVFCLDRAGLVGSDGPTHHGVFDFSYLRHIPGLVVGAP
                     RSGAELKRMLAAAFAHDGPFAIRYPAGAAEETESNGRPSTLEIGRGEVLREGSQATLV
                     AVGSCVGAAQAAADELAGDGMQVGVIDARFVKPLDTGLLEDAGRLTGLLVTVEENVLQ
                     GGFGAAVLEWAAPLGIRVHRLGIADEFVPHGSQTELRQLVGIDADSIAQAVRHAVAGR
                     VSAGP"
BASE COUNT          347 a          657 c          781 g          373 t
ORIGIN      
        1 ccactgcgac tcgatgccat caagcgggaa gcccaggcga agctcgacca ggccgagacg
       61 accatccgga agcttctgga tacgtccccg ggtgtggtgt ccgaggtgga gttcgaggcg
      121 gaggatgcat agcggcagcg accggcgtat ggtaacgaac acatgtggta cggcgatgga
      181 ggcgtgcgga tgagccgcct tctggatacc atcgacagcc ctcgtgacct gaagcgcctg
      241 tcggccgccg atctgccgcg tctggcccag gagatccgtt cagcgctcat cgagacggtg
      301 tcgatggtcg gcgggcacct ggcgcccaac ctgggcgtgg tggagctgac catcgccctt
      361 caccgggcgt tcgacgcgcc cagggaccgc atcgtgtttg atgtgggcca ccaggcctac
      421 gtacacaagc tcctgaccgg ccggcgcgag cagtttggca ccttgcggcg gcgcggggga
      481 atctcggggt tcccgcgccg ctgcgagagt ccctatgacg tgttcggcac ggggcatggc
      541 agcacatcga tctcggcggc cctgggcatc gccacagcgc gggatctggc gggtgagcac
      601 taccaggtcg tcgctgtcat cggcgacggc gccatgaccg gcggcatggc cttcgagggt
      661 ctgaaccagg tggcggaacg tgataccgat ctggtgatca tcctcaacga caacggcatg
      721 tccatctccc ccaatgtggg cgccatggcg cggtatctcc agaggttccg gctcgatccg
      781 ggctattcga gtgccaagcg cagcgtcgag gggctggtgc gccgatatga ccgtttcgga
      841 ctcggtaacc atgtactcga cgcggttgag aagctcaaag acggggccaa gagcctcttc
      901 ctgcccggaa tgttgttcga ggagctcggg ttgcggtatt tcggaccggt ggacggccac
      961 gacctcgacg agctcacgcg cactttcgaa caggtacggc gtctgaaggg gccccggatt
     1021 gtccacgtgg tgacccagaa ggggcgcggc taccggctcg cggaggagga tgccgagtcc
     1081 tatcatggct gcaagccttt cgacgtggac cagggccaac tggacccacc cgtgccccag
     1141 aagacgtact cggaagtatt cagcgaagcc ttggcggggc tggcggaggc ggacagccgg
     1201 atcgtggcgg tcacggcggc catgtgcgag ggcacagggc tgaagccctt cgcgcggcgc
     1261 ttcccccacc ggttcttcga tgtcggaatg gccgaggagc acgccgtgac cttcgcggcc
     1321 ggcctggctg ccgagggctg gcgtcccgtc gtcgccatct actcgacatt cctgcaacgg
     1381 gcctacgacc aggtgctaca cgacgtgtgc ctccagggcc tgcccgtggt gttctgcctg
     1441 gatcgggccg ggctcgtggg aagcgatggc ccgacccatc acggtgtgtt tgacttctct
     1501 tacctgcgac acatcccggg cttggtggta ggggcgccgc gcagcggggc cgagctgaag
     1561 cgaatgcttg cagccgcgtt tgcccatgat gggccgttcg cgatccgtta cccggcggga
     1621 gccgctgagg agaccgagtc caacgggcga ccgtcgacgc ttgagatcgg ccggggcgag
     1681 gtgttgcgcg aaggcagcca ggcgacgctg gtggcggtgg gttcgtgcgt gggagcggct
     1741 caggcggccg ccgatgagct ggccggagat ggcatgcagg tcggcgtgat cgatgcccgt
     1801 ttcgtcaagc cgctggacac ggggttgctc gaggatgcgg ggcgccttac ggggttgctg
     1861 gtgacggtcg aggagaacgt tctgcaaggc gggttcggtg ccgccgtgct cgagtgggcc
     1921 gctccgttgg gcattcgggt gcatcggttg ggcattgcgg acgagttcgt gccgcacggc
     1981 agccagacgg agctgcgcca gctcgtgggt atcgacgcgg acagcatcgc ccaagccgtc
     2041 cggcacgcgg tggcaggccg cgtcagtgca gggccatgaa ggtcgacgcc ttcctgcgtg
     2101 agatccgcaa ctcccgggac tacgagggcc agatggtcca cgttcaggac atcccggc
//
LOCUS       DAOSPL010000877         2157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_51044_length_2157_cov_3.202664, whole genome shotgun sequence.
ACCESSION   DAOSPL010000877 DAOSPL010000000
VERSION     DAOSPL010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51044_length_2157_cov_3.202664"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PLQ54_14745"
     CDS             <1..938
                     /locus_tag="PLQ54_14745"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2332 family protein"
                     /protein_id="HOS94576.1"
                     /translation="PEQELAQWYPNLTATPRTDDPLPAALAFCAEHRGDLVELVRTRT
                     PQTNEIGRCALLLVGLDRVATEVGALAQLDVILGDHYGDPHYFARRQSALLVGVDCLP
                     DEHCFCGQVGTARAEGKCDVFLTDVGLAYLVECWSDEGREAVEGFGGLEEVTPAVLEA
                     ARARMERKLAGIEPRLSAPVEMLPMLLDGHQKASEWEDVAAKCVSCGACNLVCPTCNC
                     FDVYDEVALSLTEAERKRRWDGCMLREFTLVAGGEVFREGKPDRLRHRMQRKYNYLYV
                     RYGSPFCTGCGRCARSCPAGIHPVSAVNQVTASES"
     gene            952..1794
                     /locus_tag="PLQ54_14750"
     CDS             952..1794
                     /locus_tag="PLQ54_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOS94577.1"
                     /translation="MAVAPVEVTPGLYTPELATVLKVTPQTETETHFLLRLNQGRSLG
                     HQPGQFVEVSVFGTGEAPISITSEPRPDDTFELCLRRAGKVTGALHRLREGDVIGIRG
                     PFGRPFDLEQFRGNDVLFVAGGLGLIPLRSLIRTVLADRSSYGGVTILYGTKQPSELL
                     YRDELAEWEARDDVDLRVTIDRRHPDWDGHVGVVTTLFPEIEIAAERTVAAIVGPPVM
                     FRFTILECTKAGIPDHNIVLSLERHMKCGVGKCGHCQINGRYVCLDGPTFRFSEIKRL
                     SEAM"
     gene            1795..>2157
                     /locus_tag="PLQ54_14755"
     CDS             1795..>2157
                     /locus_tag="PLQ54_14755"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HOS94578.1"
                     /translation="MSAKPKVAFFSFTGCEGCQLAVLELEPVLLDILGAVDIVSFREA
                     MTEHSEDYEIAFVEGSISTPHDVEEVQHIRQRAGLVVAIGACACSGGLNALKNRRGMD
                     DVLQTVYGSSGSWFETIPA"
BASE COUNT          343 a          596 c          815 g          403 t
ORIGIN      
        1 atccggagca ggaactggcg cagtggtacc cgaacctgac cgcgacgccg cgcacggacg
       61 acccgctgcc ggcggcgctc gccttctgtg ccgagcatcg cggcgacctg gtcgagctgg
      121 tgcgtacccg caccccgcag accaatgaga tcggacgctg tgcgttgctg ctggtcggac
      181 tggatcgtgt cgccaccgag gtgggtgcgc tggcccagct ggacgtgatt ttgggggatc
      241 actacggtga tccccattac tttgcgcggc gccagagcgc tctactggtc ggagtggact
      301 gtctccctga tgagcattgc ttctgcggtc aggtgggtac ggcgcgggcc gaaggcaagt
      361 gcgacgtgtt cctgaccgat gtgggcttgg cttaccttgt cgagtgctgg agcgatgagg
      421 ggcgggaggc ggttgagggt tttggcgggc ttgaggaggt cacgccggcg gttctggagg
      481 cggcacgggc gcgcatggag cggaagctgg cggggatcga gccacggctg agtgcgccgg
      541 ttgagatgct gccgatgctg ctggatggcc atcagaaggc gtccgagtgg gaggatgtgg
      601 cggcgaagtg cgtgtcgtgc ggtgcctgca atctggtgtg cccgacgtgc aactgcttcg
      661 acgtgtatga cgaggtggcg ttgagtctca cggaggccga gcgcaagcgg cggtgggatg
      721 gatgcatgct ccgtgagttc accctggtgg ccggcggcga ggtgttccgg gagggcaagc
      781 ccgaccggct gcggcaccgg atgcagcgga agtacaatta cctttacgtc cgctatggct
      841 ctccgttttg cacgggctgc ggccggtgcg cgcggagttg cccggcgggc atccatcctg
      901 tcagtgccgt caaccaggtg acggcttctg agtcgtaacg gaggatgcgc catggccgtc
      961 gccccggtgg aggtcacgcc cgggctgtat acgcccgagc tggctaccgt gctgaaggtg
     1021 acaccgcaga ccgagacgga gacgcacttc ctgctccgtc tgaaccaggg acggagcctg
     1081 gggcatcagc ccgggcagtt cgtcgaggtg tcggtgttcg gcacaggaga ggcaccgatc
     1141 tcgatcacta gtgagccccg gccggacgat acgttcgagc tgtgcctgcg gcgggcgggc
     1201 aaggtgacgg gggccctgca tcgtttgcgg gagggcgatg tgatcgggat ccgcggcccg
     1261 tttggccggc cgttcgacct ggagcagttc cggggcaacg atgtgctgtt cgtcgcgggc
     1321 gggctgggtc tcattccgct gcggtcgctg atccgcacgg ttttggcaga tcgaagcagc
     1381 tatggcggcg tgacgattct gtatgggacg aagcagccgt cggagctgct gtatcgcgac
     1441 gagctggccg agtgggaggc gcgggatgac gtggacctcc gggtgaccat cgaccggcgg
     1501 cacccggact gggatggtca cgtgggcgtc gtgacgacgc tgtttccgga gatcgagatc
     1561 gcggcggagc ggacggtggc ggccattgtg ggtccgccgg tgatgttccg gttcacgatc
     1621 ctcgagtgca ccaaggccgg gatccccgac cacaacatcg tgctctcgct cgagaggcac
     1681 atgaagtgcg gagtgggcaa gtgtgggcat tgccagatta acggccggta tgtgtgtctg
     1741 gatggtccga cgtttcgctt cagcgagatc aagcggctaa gtgaggccat gtagatgagc
     1801 gcgaagccca aggtcgcctt cttcagtttc acggggtgcg aaggatgcca gctggcggtg
     1861 ctggagttgg agccggtgct gctggacatc ctcggtgcgg tcgacatcgt gagtttccgc
     1921 gaggcgatga ccgagcacag cgaggactac gagatagcct tcgtggaggg gagcatttcg
     1981 accccgcatg acgtcgagga ggttcagcac atccgccagc gggcaggtct ggtcgtggcc
     2041 attggtgcct gcgcctgcag cggaggcctc aacgcgctca agaaccgtcg aggcatggac
     2101 gatgtgctcc agaccgtgta cgggagcagc ggatcctggt tcgagaccat acccgcg
//
LOCUS       DAOSPL010000878         2155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_51097_length_2155_cov_3.265714, whole genome shotgun sequence.
ACCESSION   DAOSPL010000878 DAOSPL010000000
VERSION     DAOSPL010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51097_length_2155_cov_3.265714"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..994
                     /locus_tag="PLQ54_14760"
     CDS             29..994
                     /locus_tag="PLQ54_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOS94579.1"
                     /translation="MVGEGDAHLVALREILGVKIIGRGSAVEVAGEADPVAWAAQVLQ
                     SALEAIRQGHPVTTADLQYAAGRARAGRPIDLAGLLADTIVRGDRGRQIRPRTAGQRA
                     YVSAMRAADLTFGIGPAGTGKTYLAVALAIAELKAQVRERIILTRPAVEAGEHLGFLP
                     GDLTEKVDPYLRPLHDALYDMVGVERARRLAERGTIEVVPLAYMRGRTLNNAFVILDE
                     AQNTTVRQMQMFLTRMGFSSKIVVTGDVTQTDLPEGQESGLVHAEGILQGIEGIRIVH
                     LTDEDVVRHPLVRRVIRAYDEAAAGRRSVGPETDGPAETVGRPEP"
     gene            1007..>2155
                     /locus_tag="PLQ54_14765"
     CDS             1007..>2155
                     /locus_tag="PLQ54_14765"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94580.1"
                     /translation="MRRLADLIRRWARVVPGRGSEEARRRLSRVLLSGAVVLSFSAAV
                     CTRVAPRALHVQVGEPSPRTVLSPRDAQFEDEEATRQAIEQARSNTPKQYDSAKVQAQ
                     SEMEADLDAVWAALRAAIDRQKGTPPAEREPDDELAQSLKQAGLDISLGAMRNLVRRS
                     PDELQALRARGREVIRREALESETRDDEPEAHEAARKRMAEACRAAGGPVGEALGEVV
                     SACFRPNMRYNDAATRAAMQRAQDQVQRHIISVKERERIVEEGQIVDQLAYEKLAHLG
                     LLQEQVDYLQLAYTATAAALSVLLFGLMFRYCVPELWANAKALTAVAGTVVGGTLITN
                     LALAPSRSMEGVAVVMAASQAIGVMCFLLFGSLPALLVIGIVSVAAAMV"
BASE COUNT          365 a          677 c          777 g          336 t
ORIGIN      
        1 cgccgcggat gccgccgagg ccgcggccct ggtgggcgag ggtgacgctc acctggtggc
       61 actccgcgag atcctgggag ttaagattat cggccgtggc agtgccgttg aggttgcggg
      121 tgaggccgac ccggtggcct gggcggccca ggttctgcag tcggcgctcg aggcgatccg
      181 ccaaggccac ccggtgacga ccgcggacct tcagtatgcc gctggccgcg ctcgcgcggg
      241 tcgacccatc gacctggcgg gactactggc ggacaccatc gtccgcggcg accgcggccg
      301 acagatccgt cctcgaacag cgggccagcg cgcgtacgtt agtgcgatga gagccgcaga
      361 cctcaccttt ggcatcggac ctgccggcac ggggaagacc tatctggcgg tggccttggc
      421 gattgccgag ctcaaagccc aagttcggga acggatcatc ctgacacggc cggccgttga
      481 ggcgggggag catctggggt tcctgcccgg cgacctgact gagaaggtcg acccctacct
      541 gcgcccgctg catgacgcgc tctatgacat ggtgggagtg gaacgagcgc ggcgactggc
      601 cgagcgcggc accatcgagg tcgttcccct ggcgtacatg cgtggacgca cgctgaacaa
      661 cgcattcgtt atcctggacg aggcacagaa cacgacggtt cgccagatgc agatgttctt
      721 gacccggatg ggcttcagct cgaagatcgt tgtgacggga gatgtgaccc agacggatct
      781 ccccgaaggc caggagtccg gcctcgtcca tgccgaggga atcctgcagg gcattgaggg
      841 gatccgcatc gtccatctga ccgatgagga cgtcgtgcgc catcccctgg ttcgacgtgt
      901 gatccgcgcg tacgatgagg ccgcggccgg ccggcgatcg gtcggaccgg agacggacgg
      961 gccggccgag accgtgggca ggccggagcc atagggaaga tgcgcggtgc gcaggctggc
     1021 tgacctgatc cgcagatggg cccgggtggt ccccggccgc gggagtgaag aggcccggcg
     1081 ccgcctttcc cgtgtgctgc tgagtggggc cgtggtattg agcttctcgg ccgcggtgtg
     1141 cacccgcgtg gccccccgcg cattgcacgt gcaggtcggc gagccctcgc cgcgaaccgt
     1201 cctctcgccc cgggatgcgc agtttgagga tgaggaagcc actcgccagg ccatcgaaca
     1261 ggcgagaagc aacaccccca agcagtacga tagcgccaag gtccaagccc agtcggagat
     1321 ggaagcagac cttgatgctg tatgggcggc gctgcgcgcg gccatcgacc ggcagaaggg
     1381 gaccccgccc gctgagcgcg agccggacga tgaactggcc cagtcgctca agcaggccgg
     1441 actggacatc agtctcggag ccatgcgcaa cctggtgcgc cggagcccgg acgagctgca
     1501 ggcgctccga gctcgcggaa gagaggtgat ccggcgagaa gcactggagt cggagacgcg
     1561 ggatgatgag ccggaagcgc atgaggcggc ccggaagcga atggccgagg catgccgcgc
     1621 cgcgggtggg ccggttgggg aagccctcgg cgaggtcgtc agtgcgtgtt tccggccgaa
     1681 catgcgctat aacgatgcgg cgacccgggc ggccatgcag cgggcgcagg atcaggtcca
     1741 gcgccacatc atctccgtca aggagcgcga acgcatcgtc gaggagggcc agatcgttga
     1801 tcagctggcc tatgagaagc tcgcgcacct cgggctactc caggagcagg tcgactacct
     1861 ccagctggcc tatacggcca cggcggccgc actcagtgtg ctgcttttcg gcctgatgtt
     1921 ccgctactgc gtcccggagc tgtgggcgaa tgcaaaggca ctgactgccg tggccggcac
     1981 ggtggtcggg ggaacgctca ttaccaacct ggcgctggcg cccagccgct cgatggaggg
     2041 cgtcgccgtc gttatggcgg ccagccaagc catcggtgtc atgtgctttc tactattcgg
     2101 gagcctgccg gccctactgg tgatcggcat cgtgagtgtc gccgcggcca tggtg
//
LOCUS       DAOSPL010000879         2154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_51134_length_2154_cov_2.946641, whole genome shotgun sequence.
ACCESSION   DAOSPL010000879 DAOSPL010000000
VERSION     DAOSPL010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51134_length_2154_cov_2.946641"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2093)
                     /locus_tag="PLQ54_14770"
     CDS             complement(<1..2093)
                     /locus_tag="PLQ54_14770"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HOS94581.1"
                     /translation="MLITYNEHETNAVMARFSPGEAPPTEARGGRSFSHLGNFNGWRL
                     VHLVSRQGEIQSLQSTSKAIEVWEPAAVIGIGIAFGVDDAKQAIGDVLISEAIQGYDL
                     ARANRDGKLTPRETAWPASERLLQRLLACHHQPRATTQWPTLHFGVVLSGSKLVDNLD
                     YRNSLVALFPQKIVGGEMEGVGLAAACNDAKVDWVVVKGICDWADGKKNTGSKDRDQR
                     LAADNAVAVVAMALSESPQPSATPGKPGDDDVGRRPRLPLAPSAHLMGMRDLGTIEPR
                     LLIDTPLGLPSTMDKEDPAPQRAFDPDARTLDVASQHGGGVDVMTALHAWVDETDAPP
                     VFAILGEYGMGKTVTAQRLARELEAERQEDPTRPAVLYFDLRSVTGLDKGVPTLAQTV
                     EECMARSWIADEDPAAYTWSNVLAWLTAGAVVIFDGLDEVLVKLDGGDGQTFTRTLLS
                     IVDEQRRRSGTARVLVTCRTQYFRTLREQSNHFSGQERGNVRADSYRSLGLLPLTEAQ
                     VLHYLAAAVPGSRPDDLLDMLRSVHDLTDLTHRPYTLTLVSQFLPEIEADRAAGRTVY
                     GVTLYRRMVARWLERDAGKHHLRPEDKEELAQHLAAHLHSAGAALLAATDLEDWMHKW
                     LEATPRLARRYRRLHPDQLEEDLRTATFLARIDGSDGSGFRFAHTSLAEYFHACHLLR
                     TIETDNPHAWDITPPS"
BASE COUNT          348 a          658 c          751 g          397 t
ORIGIN      
        1 gacggtgggg tgatgtccca ggcatgaggg ttgtcggtct cgatagtccg cagcaagtgg
       61 cacgcgtgga agtactcggc cagagacgtg tgggcgaagc gaaagccgct gccgtcggac
      121 ccgtcaatcc gcgcgaggaa ggtggccgtg cgcaggtctt cctcaagttg gtcggggtgt
      181 aggcgcctgt atcggcgggc cagccgagga gtggcctcga gccacttatg catccagtct
      241 tcaagatcgg tggcggcgag caatgcggct ccggcgctgt gcaggtgcgc cgcgaggtgt
      301 tgggcgagct cttccttatc ctcgggccgc aggtgatgct taccggcatc gcgctccagc
      361 cagcgggcga ccatgcgccg gtaaagggtc acgccgtaaa cggtccgacc ggccgctcgg
      421 tcggcctcaa tctcggggag gaactggcta accagggtaa gcgtgtacgg ccggtgggtc
      481 aggtcagtga ggtcgtgaac gctgcgcagc atgtcgagca ggtcgtcggg tcggctaccc
      541 ggaactgctg cggcaagata gtggaggacc tgcgcctcag tgagtggcag cagcccgagg
      601 gagcgatagg agtccgcacg cacgttgccg cgttcttggc cggagaagtg attggactgt
      661 tcgcgcaacg tgcggaagta ctgcgtgcga cacgtgacga gcacgcgtgc ggtgccgctg
      721 cgacggcgct gctcgtcgac gattgagagc aaggtgcggg taaaggtctg cccgtcgccg
      781 ccgtcgagtt tgacgagcac ctcgtcgagg ccgtcgaaga tgacgacggc gccggctgtc
      841 agccaggcca ggacgttgct ccacgtgtat gccgccgggt cctcgtcggc gatccaggag
      901 cgggccatgc actcttccac cgtctgggca agggtcggaa cgcctttgtc gaggccggtc
      961 actgagcgca ggtcgaagta gaggacggct ggccgggtgg ggtcctcctg gcgctcggcc
     1021 tccaactcgc gagccagccg ctgagcggtc accgtcttac ccatgccgta ttcaccaaga
     1081 atcgcaaaca cgggtggagc gtccgtttca tcgacccacg cgtgcaacgc ggtcatgacg
     1141 tcgaccccgc cgccatgctg ggacgccacg tccaaggtgc gcgcgtcggg gtcgaaggct
     1201 cgttggggtg cggggtcttc cttgtccatt gtcgatggca ggccgagcgg ggtgtcgatg
     1261 aggagtcgcg gctcgattgt gccaaggtcc ctcatcccca tcagatgggc agaaggcgcg
     1321 aggggtagcc gcgggcggcg acccacatca tcgtcgcccg gcttgcccgg ggttgcgctt
     1381 ggctgtgggc tctcgctgag tgccattgcg accacggcga ccgcgttgtc cgcagccaag
     1441 cgctgatccc gatccttgct gcctgtgttc ttcttcccgt cggcccaatc gcagatacct
     1501 ttgaccacaa cccaatcgac cttggcgtca ttgcaggctg cagcaaggcc gactccctcc
     1561 atctcgccgc cgacgatctt ctgggggaac agggccacga ggctgtttcg atagtccaag
     1621 ttgtcgacaa gtttgctgcc gctcaacacg acaccgaagt gcagggtggg ccattgggtc
     1681 gtggcccgag gttggtggtg gcaggcgagg agccgctgca gcagccgttc cgacgctggc
     1741 cacgcggtct cgcgcggcgt gagcttcccg tctcggttgg cacgagccaa gtcgtaaccc
     1801 tggatcgcct cgctgatcaa cacatcgccg atggcttgct tcgcgtcgtc gacgccaaag
     1861 gcgatgccga tcccgatgac agccgccggt tcccaaactt cgatggcctt gcttgtcgac
     1921 tgtaacgatt ggatctcgcc ttgcctgctg acgaggtgca cgagccgcca cccgttgaag
     1981 ttgcctaagt gcgagaaaga gcgcccgccg cgggcctccg tgggcggagc ctcccctggg
     2041 ctgaaacggg ccatgaccgc atttgtctcg tgctcgttgt aggtgatgag cacgacgaca
     2101 ggcgcgcctg ggtcggtgcc gcgggtgtcg accagccggt tcgctgggat cgag
//
LOCUS       DAOSPL010000880         2153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_51163_length_2153_cov_2.681602, whole genome shotgun sequence.
ACCESSION   DAOSPL010000880 DAOSPL010000000
VERSION     DAOSPL010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51163_length_2153_cov_2.681602"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PLQ54_14775"
     CDS             <1..392
                     /locus_tag="PLQ54_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94582.1"
                     /translation="ISARFGGRKVEFKGRQAGRPPIDQTLTLPEGCHVFENNCMMAWM
                     LWCSQVSLTPGSHSQTEFFHLETMQVMPLQFDVRAKAGERKVDGKRVATHVIDVAPIG
                     NTFYVTIPDRRFVGLIASGGISIKLVN"
     gene            complement(411..1892)
                     /locus_tag="PLQ54_14780"
     CDS             complement(411..1892)
                     /locus_tag="PLQ54_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015146028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HOS94583.1"
                     /translation="MGKPTGFMEYQRELPADRPPLDRLRDWNEFHGAMPEDGLRTQGA
                     RCMDCGTPFCHAGILLEGMASGCPINNLIPEWNELVYLGLWEMALERLHKTNNFPEFT
                     GRVCPAPCEEACVVAIHSPAVAIKNIECHVIERGFAEGWVRPRPPAVRTGKRVAVVGS
                     GPSGLACAAQLNSAGHSVTVYERNDRIGGLLVYGIPQMKLEKRIVERRLDLMRAEGIE
                     FIPNTWVGRDLPASHLVEHNDAVVLCIGATRPRDLAVPGRELAGVHFAMDFLTRNTRA
                     LLSGGTNGSLISAAGKDTIVIGGGDTGTDCVGTALRHGCRSLAQFEILPEPPTERGPG
                     NPWPQWRKILRRDYGQEEAQARFGRDPREYSIMTKLLVGDAKRHVKEVHTVRVQWVPG
                     ADGRLEPREVPGSEQIWPAQLVLLAMGFVGPEDDVIEQLGLERDARTNIRAEYGQYAT
                     SVPGVFAAGDARRGQSLVVWAINEGRQAAREVDRYLMGSTQLP"
     gene            complement(1947..2123)
                     /locus_tag="PLQ54_14785"
     CDS             complement(1947..2123)
                     /locus_tag="PLQ54_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94584.1"
                     /translation="MFSSNDTSAGRFVKVYPKDYRRAQEAMARIEAMGLSGDEAVMAA
                     FEENKRDVARVSGS"
BASE COUNT          352 a          712 c          720 g          369 t
ORIGIN      
        1 ccatcagtgc ccggtttggg ggccgcaagg tggagttcaa gggcaggcag gcggggcggc
       61 caccgatcga ccagaccctc acgctacctg agggctgcca tgtattcgag aacaactgca
      121 tgatggcgtg gatgctgtgg tgcagccagg tctcactcac gcccgggagc cactcgcaga
      181 cggagttctt ccacctggag acgatgcagg tcatgccgct ccagttcgac gtgcgcgcga
      241 aagccgggga gcgcaaggtc gacggcaagc gagtcgccac gcatgtgatc gacgtcgcac
      301 cgattgggaa cacgttctat gtgaccatac cagaccggcg attcgtgggc ctcatcgcca
      361 gcggcggcat cagcatcaag ctcgtgaact gatgccaagc ggctggccag ctaaggcagt
      421 tgcgtcgacc ccattagata gcggtccacc tcacgtgcgg cctgtcgccc ctcgttgatg
      481 gcccacacga ccaggctctg gcctcggcgg gcgtcgccgg cggcgaacac accggggacg
      541 ctggtggcgt actgaccgta ctccgcgcgg atgttcgttc tcgcatcccg ctccaggccg
      601 agctgctcga tcacatcgtc ctcgggcccc acgaatccca tggccagcaa cacaagttgg
      661 gcgggccaga tctgctcgct gccgggcacc tcccgtggtt cgagccggcc atcggcgccc
      721 ggtacccact gcacgcggac ggtgtggacc tccttgacgt gacgctttgc gtcgccgacg
      781 agcagcttgg tcatgatgga gtactcgcgg gggtcgcgcc cgaagcgcgc ctgggcctcc
      841 tcctggccgt agtcgcggcg gaggatcttc cgccactggg gccaggggtt gccgggaccg
      901 cgctccgtcg gaggctcggg caggatctcg aactgcgcca ggctgcggca gccgtgccga
      961 agggcggtgc ccacacagtc ggttcctgta tcaccaccgc caatgacgat agtgtccttc
     1021 ccggcggccg agatgaggct accgttggtt cccccgctca gcagtgcgcg ggtgttccgc
     1081 gtgaggaaat ccatcgcgaa atggactcct gccagctccc gcccgggcac ggccaggtcg
     1141 cgcgggcgcg tggcgccgat gcacagaacc acggcatcgt tgtgctcaac caggtggctt
     1201 gccggcaggt cccggccgac ccacgtgttc gggatgaact cgatgccttc ggctcgcatg
     1261 aggtcgagcc ggcgctcgac gatgcgcttc tcgagcttca tctgcgggat gccgtacacc
     1321 aggaggccac cgatgcgatc gttgcgctcg tagacggtca ccgagtgacc agcggagttc
     1381 agttgcgcgg cgcaagcgag acccgatggg ccagacccca cgacagccac ccgcttcccc
     1441 gtccgtacgg cggggggccg gggccgcacc cagccctcgg cgaaaccgcg ctcgataacg
     1501 tggcactcaa tgttcttgat cgctactgcg gggctgtgaa tggcgacgac gcacgcttcc
     1561 tcgcacggag cgggacagac gcggccggtg aactcgggga agttgttggt cttgtgcagg
     1621 cgctccagcg ccatctccca gagcccgagg tagacgagct cgttccactc agggatcagg
     1681 ttgttgatgg ggcagccaga ggccatacct tcaagcagga ttccggcgtg gcagaaaggc
     1741 gtgccgcagt ccatgcaccg ggcgccctgg gtccggagcc cgtcctcggg catggcgccg
     1801 tggaactcgt tccagtccct cagccggtcc agcggcgggc gatccgccgg gagttcgcgt
     1861 tggtattcca tgaagccggt tggcttaccc acggtcgtct cctgttcgtg ccagcgagag
     1921 gggtacgcgc cgggtctggg cgtgaactag ctgccgctga ctcgcgccac gtctcgcttg
     1981 ttctcctcga atgccgccat gacggcttca tcgccgctca ggcccatggc ctcgatccgg
     2041 gccatggcct cctgggcgcg gcggtagtcc ttggggtaga ccttcacaaa ccggccggca
     2101 ctggtatcat tcgaggagaa caagcgagac gtggcgcgag tcagcggcag cta
//
LOCUS       DAOSPL010000881         2152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_51185_length_2152_cov_2.982356, whole genome shotgun sequence.
ACCESSION   DAOSPL010000881 DAOSPL010000000
VERSION     DAOSPL010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51185_length_2152_cov_2.982356"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..784
                     /locus_tag="PLQ54_14790"
     CDS             116..784
                     /locus_tag="PLQ54_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS94585.1"
                     /translation="MQALRGSTGGAEVANRTRSPGFTLIELLVVIAIIAILAAILFPV
                     FAKARDKARVATCISNCKQLLYGALMYASDYDGELPSDYHAGNPHALLISKLWPYTKA
                     MGIYYCPSAPAAQVADIVCNEQNQAAGNISYYWFCFSELPSTGVLPMQWVDKQFLTNP
                     NNWGNKPRQVTDGWEPTTWIVSDYFYKETAYPVHVGHRRAMVVGYLDGHAKFLVQQPT
                     TQFK"
     gene            807..>2152
                     /locus_tag="PLQ54_14795"
     CDS             807..>2152
                     /locus_tag="PLQ54_14795"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOS94586.1"
                     /translation="MSRRQIRAANAAALAVVWTTATVLLAGQGLRDRKALVVWSCGGN
                     YEFLSDFVRRFETAHGIRVCYTAAPVQYLLDLTLRGPRKPDVIVGRGGPGWLALEKRG
                     LLAAEPTFFAVDPMVIAVNPGCAQPITCIEDLGKPGVRVAAAPAAMRPKGKVVGQLMA
                     SISAAESPGLVERWQANMVDSPKCGRHLLDPLRQGVADVAVVPLSLTPSATAQGTVRI
                     VSIAPRHLVRMKEGRASMPQCAAALRTSTRPEAAARFVAALADPRSAELLKRAGYIPT
                     ASPEANPYRPMLELSAPRDMPGWQVRLAQMLVEHNAIPAAARRYLVAVCLFGPSIYDG
                     RALCELGDLLHAQGQTDAAHRVWAHAAEILPRPLPNEFSSDALQCLGGVPGIAWYDDS
                     HWASVARDRLARDGATGTRPPAWRPVDESAPPKGGTRQMALAERLASLGYVEAAVK"
BASE COUNT          360 a          783 c          639 g          370 t
ORIGIN      
        1 gctcgcctca ttcgttcgcc acaggcgcag tgcccccacc agccggcctc gcgtatacct
       61 gtgcttccca gattccgttg tcaggaatcc tgatatcaat gcgaattgca tatagatgca
      121 agcccttcgc gggtcaaccg gaggtgcaga ggtggccaac cgaactcgct caccaggctt
      181 tacgctcatc gaactccttg tcgtcatcgc catcatcgcc atcttggcgg ccattctctt
      241 cccagtgttc gccaaggcca gggataaggc ccgtgtggcc acctgcatct cgaactgcaa
      301 gcaattgctc tacggcgcgc tcatgtacgc gtccgactac gacggcgagc taccgtccga
      361 ctaccacgcc ggcaatcccc atgccctcct catcagcaag ctctggccct acaccaaggc
      421 catgggcatc tactactgtc cgagcgcacc ggccgcccag gtcgccgata tcgtctgcaa
      481 cgagcagaac caggccgcag gcaacatcag ctactactgg ttctgtttct ctgaactccc
      541 cagtaccggc gtactgccca tgcagtgggt cgacaagcag tttctcacga acccgaacaa
      601 ctggggcaac aagccccggc aggttaccga cgggtgggaa cccaccactt ggatcgtctc
      661 cgactacttc tacaaagaaa cggcctaccc cgtgcatgtc ggtcaccgtc gcgccatggt
      721 tgttgggtac ctcgacgggc atgccaagtt cctggtgcag caacccacca cccagttcaa
      781 gtgaggcatg attcgcagga ggtcccatga gccggcgaca gatccgcgcg gctaacgcgg
      841 cggcgttggc agtggtgtgg acgaccgcga cagtactcct ggccggccag ggcctccgtg
      901 accggaaggc tcttgtggtc tggtcttgtg gcggaaacta cgagttcctg tcagacttcg
      961 tgaggcgatt cgagaccgcg cacggcattc gcgtctgcta caccgctgcg ccggtgcagt
     1021 atctcctcga tctcacactg cgcggccccc ggaagcccga tgtcatcgta gggcgcggtg
     1081 ggccgggttg gctggctctc gagaagcgcg gactcctggc ggccgagccc acgttcttcg
     1141 cggtcgaccc gatggtcatc gccgtcaacc ccggttgcgc tcagcccatc acctgcatcg
     1201 aggacctcgg gaagcccggc gtacgcgtgg ctgctgcgcc cgcggccatg cgccccaagg
     1261 gcaaggtcgt cgggcagctc atggcctcga tcagtgccgc cgaaagcccc ggccttgtcg
     1321 agcggtggca ggccaacatg gttgactcac ccaagtgcgg gcgccacctg ctcgatccgc
     1381 tccgccaggg agtcgccgat gtcgccgtgg tccctctctc cttgacgccc tctgccacgg
     1441 cccaagggac cgtacgcatc gtgtccatcg caccccggca tctggtccgg atgaaagagg
     1501 gccgggcctc aatgccccag tgcgccgccg ccctgcgaac cagcaccagg ccagaggccg
     1561 ccgcgcggtt cgtcgctgca ctggccgatc cccgatcggc cgaactcctg aagcgggcgg
     1621 gctacatccc caccgcatca cccgaggcaa acccttaccg gcccatgctt gagctctccg
     1681 cgccccgcga tatgcctggc tggcaggtcc gcctcgccca gatgctcgtc gaacacaacg
     1741 ccatccccgc cgccgcgcgc cggtacctgg tggcggtctg cctgttcggg ccctcgatat
     1801 acgacggccg ggccctgtgc gagttgggcg acctcctcca cgcccagggc cagaccgacg
     1861 ccgcccaccg ggtctgggcc catgcggccg agatcctccc gaggcccctg cccaacgagt
     1921 tctcgtccga tgccctccag tgcctgggcg gcgtcccggg tatcgcgtgg tacgacgaca
     1981 gccactgggc atccgtggcc cgggatcggc tcgcccgcga tggcgcgacc ggcacccgtc
     2041 cacccgcctg gcggcctgtc gatgagagcg ccccgcccaa agggggcacc cggcagatgg
     2101 cgctggccga gcggctcgca tccctcgggt atgttgaagc cgccgtcaag ga
//
LOCUS       DAOSPL010000882         2150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_51270_length_2150_cov_2.333652, whole genome shotgun sequence.
ACCESSION   DAOSPL010000882 DAOSPL010000000
VERSION     DAOSPL010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51270_length_2150_cov_2.333652"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="PLQ54_14800"
     CDS             complement(<1..1131)
                     /locus_tag="PLQ54_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94587.1"
                     /translation="MRTSSCAAGPLLALVVAVAPAWAAGEWAAETAHRLLAAFPDCSG
                     AVVAEHPRCPEVLVDTRGTLSDTCDEIALRNTLNWRRVGDLVVLAAPPTHLIPWPSMD
                     LGTLAASSAGEDALAYALVGRPWSALSADWIHSDDGAAWRAAVDAGVLRSGELGEPLR
                     RSCEQWLSVLGLLGVGDTIGEWSVRARLWHSPIAASVRGPLAQAVGLCGDVPAGPAPI
                     TVDAYGTLRDVLGQLQSAWGRPLVAGDEIGAIPVAAAVRDTRADEVLCAVGYAVAGEW
                     RDLPGVLFLSAHPGGLRFHQRKWFWRASEGLKDAPRLSPTGARTVLIAVEPQTGGRLH
                     ALSQLYPVLSLETLQGATQGAAMLVQLAPSQAVLWAREEGFDP"
     gene            complement(1118..>2150)
                     /locus_tag="PLQ54_14805"
     CDS             complement(1118..>2150)
                     /locus_tag="PLQ54_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94588.1"
                     /translation="GVLLAQTEEPGAPGPPLSLGELRSAIVEHRSSPFPIVARVTRVM
                     GLTQAGPAARPGKDYEVLLTSMLAAVGPGVFYTEGWFDVQVRAADGVTPAPPPNAGQA
                     IKYWHAADGRIYLMRPVAALELKDPAYGVQMWAEVLPEPDDGYTLGAEQALQVPVGAP
                     GALARSYADVLGSAEARVSQETLEGLGCVRVDAVWADRKSHRTLWFAPERDYVCMRDE
                     SVYVRGEFVAVAIRTATECRQVEGAWFPSLAEHVCYRRPSGGGEWWILSVDTVVSRDV
                     EPLPDRGQLFLPIFPNATAVYRPEAGGKRDVVGVASENLLQAMASGDTILACPPAPEL
                     LGDRTYAYE"
BASE COUNT          368 a          802 c          702 g          278 t
ORIGIN      
        1 cgggtcgaaa ccctcctctc gcgcccagag aacggcctgc gagggcgcga gctgcactag
       61 catcgcggcg ccctgggttg cgccctgcag cgtctccagc gacaggacgg ggtacagctg
      121 ggagagggcg tgtagtcgcc cgccggtctg gggctccacg gcaatgagga ccgtgcgcgc
      181 gccggtcggg ctgaggcgag gagcgtcctt cagcccctcg gatgcccgcc agaaccactt
      241 gcgttgatgg aaacgcagcc cgccaggatg ggcactgagg aacagcactc ccgggagatc
      301 gcgccactcc cccgccacgg catacccaac agcgcagagc acctcgtctg ctcgtgtgtc
      361 gcgaaccgct gccgccacgg ggatggcccc gatctcgtcc cccgcaacca gggggcgccc
      421 ccaagccgat tgcagttggc ccagcacatc cctgagtgtc ccgtaggcgt ccacggtaat
      481 tggggcaggg ccggccggca cgtcaccgca cagcccaacc gcctgagcca gaggcccccg
      541 gaccgacgca gcgatgggcg agtgccacaa cctcgcccta acggaccact caccaatggt
      601 gtcgccgaca ccaagcaggc ccaggacgga gagccactgc tcgcaggatc gccgcagcgg
      661 ctcgcccagc tcgccgctcc gaagtacgcc tgcgtcgact gcggcccgcc atgcggcccc
      721 gtcgtcgctg tggatccagt cagccgacag cgccgaccaa gggcgaccga cgagagcgta
      781 ggcgagggcg tcctccccgg cggacgaggc agccagggtc cccaagtcca tcgacggcca
      841 cgggatgagg tgcgtggggg gcgcagccag cacaacgagg tcccccacac ggcgccagtt
      901 cagggtgttc cggagcgcga tctcgtcgca tgtgtcactc agggtgcctc gagtatccac
      961 cagcacctcg gggcaacgcg ggtgctcagc cacaacggcg ccgctgcagt cagggaaggc
     1021 agcgagcagg cggtgcgcgg tctcagctgc ccactcgcct gcggcccacg cgggagcgac
     1081 cgcgacaacg agcgcaagca gcggtccggc ggcgcagcta ctcgtacgca taggtcctat
     1141 ccccaagaag ctccggtgca ggcgggcagg ccaggatcgt gtcgccgctg gccatcgcct
     1201 gcaggaggtt ctccgacgcg acgcccacca catcgcgctt ccccccggcc tcgggacggt
     1261 acacggccgt ggcgttcggg aagatgggga ggaagagctg gcccctgtcg ggcagcggct
     1321 ccacatcacg gctgacgacg gtgtcgaccg acaggatcca ccactccccc ccgccggatg
     1381 gcctgcggta gcagacatgc tccgcgaggg acgggaacca cgccccttcg acctgccggc
     1441 attccgtggc tgtcctgatc gccacagcca cgaactcccc gcggacgtag acggactcgt
     1501 ccctcatgca tacgtagtcg cgctcaggcg cgaaccacaa ggtccggtgc gacttcctgt
     1561 cggcccagac cgcgtccacg cgaacgcacc caagcccttc gagggtctcc tgactcaccc
     1621 gcgcctcggc actcccaagc acatcggcgt atgagcgggc caacgcccct ggcgcgccga
     1681 cgggaacctg aagcgcctgc tcagccccca gtgtgtagcc gtcgtccggc tcaggcagca
     1741 cctcggccca catctgaacg ccgtaggcgg ggtccttgag ctcgagcgcg gcaacaggcc
     1801 gcatgaggta gatccgaccg tcggccgcat gccagtactt gatcgcctgg ccggcgttcg
     1861 gtggcggagc gggcgtcaca ccgtcagctg ccctcacctg aacgtcgaac cacccctcgg
     1921 tgtagaacac gcccgggccc acggcagcca gcatgctcgt gagcaagacc tcatagtcct
     1981 tgcccggccg ggccgccggc cctgcctgcg tcagccccat cacccgagtg acgcgcgcca
     2041 cgatcgggaa gggggagctg cggtgctcca caatcgcact gcgcagctcc cccaagctga
     2101 gcggcgggcc gggtgcgcca ggctcctcgg tctgcgccag cagcacgccc
//
LOCUS       DAOSPL010000883         2148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_51322_length_2148_cov_6.385571, whole genome shotgun sequence.
ACCESSION   DAOSPL010000883 DAOSPL010000000
VERSION     DAOSPL010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51322_length_2148_cov_6.385571"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PLQ54_14810"
     CDS             complement(<1..447)
                     /locus_tag="PLQ54_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94589.1"
                     /translation="MVFWSEAYLCALLLYEVYDPQGKRMGRLQDVLAQPNGRFPEVTK
                     IAVRRKGTSSIEYYPWSAVRTASPEGIRLNAVEPVQPSETEIHLRRDLVDKQIVDVSG
                     KRVVRVHDLKLGAIGGQVLLTHADVGWRGVLRRLGWEHFTLHLCRAL"
     gene            1513..>2148
                     /locus_tag="PLQ54_14815"
     CDS             1513..>2148
                     /locus_tag="PLQ54_14815"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94590.1"
                     /translation="MTPTLTIMALCAASGITVAQPEGPLLHYTFDADAPGQCLDSSGN
                     GLNAQGGGPRVASPSGQAMATDGTGATVAQVTVPENLALAKASWTFAAWYRPERLAIE
                     DAQNQRRIFSYGSFPDANIVIDITGAGAVTTYMCYRDAEGSIVSAGGGSSLRLSENEW
                     AHLAVVCDRDERTVSVYVNGYRSGLAGLPEGFDGDYSQHRELTIGSGWHNIQ"
BASE COUNT          359 a          757 c          682 g          350 t
ORIGIN      
        1 cagcgccctg cagagatgca gggtgaagtg ctcccaccca agacggcgga gcacaccgcg
       61 ccagcccacg tcggcgtggg tcagcagcac ctggccgccg atggcaccca gcttcagatc
      121 atggacgcga accacacgct tgccggacac gtcaacgatc tgcttgtcga ccaagtcgcg
      181 gcggaggtgg atctcggtct cgctgggctg cacaggctcg acggcattca gccggatccc
      241 ctcgggtgag gccgtgcgca cggccgacca gggatagtac tcaatcgagg acgtgccctt
      301 tcgcctcacg gcgatcttgg tgacttcggg gaatcggcca ttgggctggg ccagcacgtc
      361 ctggaggcgc cccatgcgtt tgccttgggg gtcgtagacc tcatacaaga gcagcgcaca
      421 gagatatgct tcggaccaga acaccatcgg tctcaccccc gaagtagtgc ccgcagggca
      481 cgaagaaacc cgcccggccc gggccgagcg ggagggatga gacgacggtt atttcatgct
      541 aaatcccgtc ggctcgactc accacccgac caccggagcc gcaaacggac cggcccgcgc
      601 cggacatcaa gctcgagcgt ctactgcaac tatcgcctga atcggtgtta ttctgcatag
      661 ttagtatcat caggcccgct cacccacgcc cgctcgcgcg gcagcgccca gtgaaagggc
      721 catccagcgc attctacgcc tggcgcgaag gagggtcaag cgcgatccga cgcgcggaaa
      781 cccgtgacat tcacctattt catagtttct gaaataggtg actgtcacga atggtccgca
      841 cttaagccca atgccagagg cgcttgccac atcacctctg tagccggggc tttagcctcg
      901 gcttgccaca tcacctctgt agccggggct ttagcctcgg ctcacaatgg cctgtagagc
      961 cacttcgcgc gaggctgaag cctcgcctac ggttgggtgt gcgtgggtgc cgaccctaag
     1021 tgcgtgccat tcgtggctgt caccggtttc cgcgcctcac cgccgggcta gcctgaggac
     1081 cgtcagcgcc tctgcggtga cccactcgtt catccgcgac ttgcgcgcgc cgccccagtc
     1141 caccagtgac ttgtggcagc ccgtggcggg gtcgtcccgt ccgccgggta cccgatgtcc
     1201 gcgagcagtg acagcacccc gtgcgcgccg cgccactggt tgtaggggtg gagctcgtcc
     1261 cgttcctcgg cgtggggcga cccgatcagg gtttgtgccc tggccggcgc gcatcgcctc
     1321 ccgctttcca acccttggca tcggcgacac tcacggcgcc ggcctggtcc ggcgcgaccc
     1381 gcttgcctcg cggtgcccgg taggccgagg gcccagctcc ggcgaaccca agtccgtcga
     1441 ggcggccacg gcgctgccat atggcggcgc tggcgggccc cacctcagca ccacgcagcc
     1501 aaggaggact ccatgacccc caccctcact atcatggccc tgtgcgccgc ctcgggcatc
     1561 accgtcgccc agccagaggg tccgctgctc cactacacct tcgatgccga cgcgccgggc
     1621 cagtgtctcg actcgtcggg caacggcctg aacgcccagg gcggcggccc gcgcgtcgcc
     1681 tcgccctccg gacaggcgat ggcgacggac ggcacgggcg ccacggtggc ccaggtgacg
     1741 gtgcccgaga acctggcact cgcgaaggcc tcgtggacct tcgccgcgtg gtaccgcccg
     1801 gagcgattgg ccatcgagga tgcccagaac cagcgccgca tcttctcgta cgggtccttc
     1861 cccgatgcaa acatcgtcat cgatatcacg ggcgcgggcg cggtaacgac ctacatgtgc
     1921 taccgcgacg cggagggcag catcgtctcc gccggcggtg gttcgtcgct caggctcagc
     1981 gagaacgagt gggcgcacct ggcggtcgtc tgcgaccggg atgagcggac ggtctccgtc
     2041 tacgtcaacg gctatcgcag cgggctggcg gggcttcccg agggcttcga cggcgactac
     2101 tcgcagcacc gcgagctgac gatcggcagc ggctggcaca acatccag
//
LOCUS       DAOSPL010000884         2146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_51391_length_2146_cov_2.371114, whole genome shotgun sequence.
ACCESSION   DAOSPL010000884 DAOSPL010000000
VERSION     DAOSPL010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51391_length_2146_cov_2.371114"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="PLQ54_14820"
     CDS             complement(<1..1311)
                     /locus_tag="PLQ54_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOS94591.1"
                     /translation="MARIGVFVCHCGTNIAKNVDVSRVVAAVGRLPNVVHATEYKYMC
                     SEPGQQMIRQAMEEHKLDRVVVAACSPHLHERTFRACASQAGLNPYLVEMCNIREQCS
                     WCHKDPERATQRAYEQIAMTVAKAYYDQPLTQNRLPVERRALVIGGGIAGIQAALDIA
                     DAGHPVVLVERSPSIGGRMAQFDKTFPTLDCSACILTPKMVDVAAHPNITLRTYAEVE
                     ELNGFVGNFEAKIRLKARSVRLEECKGCGECWERCPAKRQNIPSEFDAGLSTRAAIYT
                     PFPQAVPNVPVIDREHCLYFQTGKCGVCAKRCPVGCIDFEMQDEILTERFGAVVVATG
                     FDVMDPRVYGEYGGGQYANVITGLEFERLMNASGPTGGHLVRPSDHKEPKTVVFVQCV
                     GSRDQDKGHEYCSRVCCMYTAKHATLLKDKMPDAEAYVFYIDIRA"
     gene            complement(1311..2144)
                     /locus_tag="PLQ54_14825"
     CDS             complement(1311..2144)
                     /locus_tag="PLQ54_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HOS94592.1"
                     /translation="MSGTSKEYGASTVEVCRALGIELTELEDWNCCGATSGHSASREV
                     ADALGARNVLLATDMGLDLVAPCAACYNRTKRAANVLAEGDAARKQRIAELLGRPVRE
                     PVRVLHLLEVFSHEEVAERLGGRAMQPLPVSKVAAYYGCLLVRPPKETGFDDPENPQT
                     MDRLLRLCGLETCDWYFKNECCGASHSIPQSSVVLTLVDRIIRNARAAGAEWIVTACP
                     LCQVNLDTRQQVALGEEPLPVLYITQVLGLALGLSPKSLGVPEQLMIPRVPAGAAGGG
                     R"
BASE COUNT          371 a          776 c          647 g          352 t
ORIGIN      
        1 tgcccggatg tcgatgtaga acacataagc ctcggcgtcc ggcatcttgt ccttcagcag
       61 ggtggcgtgc ttcgccgtat acatgcagca cacccgcgag cagtactcgt ggcccttgtc
      121 ctggtcccgc gagcccacgc actggacgaa caccaccgtc ttgggctcct tgtgatccga
      181 cggccgaacc aggtggcccc cggtcgggcc cgaagcgttc atcagccgct cgaactcgag
      241 ccccgtgatg acgttcgcat actgccctcc cccatactcg ccgtagaccc gcggatccat
      301 cacatcgaac ccggtggcga ccaccacggc cccaaaccgc tccgtgagga tctcgtcctg
      361 catctcaaag tcgatacaac ccacggggca gcgcttggcg cacacgccgc acttgcccgt
      421 ctggaagtag aggcagtgct cgcggtcgat gaccggcacg ttcggcacgg cctgagggaa
      481 cggcgtgtag atggcggccc gggtgctgag ccccgcgtcg aactcgctgg ggatgttctg
      541 acgcttggcc gggcaacgct cccagcactc gccgcagccc ttgcactcct ccagacgcac
      601 cgaccgcgcc ttgaggcgaa tcttcgcctc gaagttgccg acgaaaccgt tgagctcctc
      661 gacctcggca tacgtgcgca gcgtgatgtt ggggtgtgcc gccacatcca ccatcttggg
      721 cgtcaggatg cacgccgagc agtcgagggt ggggaaggtc ttgtcgaact gcgccatgcg
      781 cccgccgatg gacggtgacc gctccacgag caccaccggg tgacccgcgt cagcgatgtc
      841 gagcgcggcc tggatccccg cgatgccgcc accaatgacc agtgcgcgtc gctccaccgg
      901 caagcggttc tgcgtgagcg gctggtcata gtaggccttg gccaccgtca tggcgatctg
      961 ctcgtaggcc ctctgcgtcg ccctctcggg atccttgtgg caccacgaac actgctcacg
     1021 gatgttgcac atctcgacca gatacggatt cagtcccgcc tgggacgcac aggcccggaa
     1081 agtccgctcg tgcaggtgcg gcgagcaggc cgccaccacc acgcgatcca gcttgtgctc
     1141 ctccatggcc tggcgaatca tctgctggcc aggctccgag cacatgtact tgtactccgt
     1201 ggcgtggacc acgttcggca accgcccgac ggcggccacc acgcgggata catccacgtt
     1261 cttggcgata ttggtcccac agtggcacac gaacacgccg atgcgcgcca ctaccggcca
     1321 cctcctgccg ccccggccgg gacgcggggg atcatcaact gctcgggaac acccaaagac
     1381 ttcggcgaca ggcccagggc cagaccaagc acctgggtga tgtacagcac cggcagcggc
     1441 tcctcgccca gggcgacctg ctgccgcgta tcgaggttca cctgacacag tgggcaggcc
     1501 gtaacgatcc actccgcgcc cgcggcgcgg gcattccgta tgatccgatc gaccagcgtc
     1561 aggaccaccg agctctgggg gatcgaatgg ctggccccgc agcactcgtt cttgaaatac
     1621 cagtcgcagg tctcgaggcc acacagccga agcagacggt ccatggtctg tggattctcc
     1681 ggatcatcga acccggtctc cttgggcgga cgcacgagaa ggcacccgta gtacgccgcg
     1741 accttcgaaa ccggcaacgg ctgcatggca cgccctccga gccgctcggc cacctcctcg
     1801 tgcgagaaca cctccagcag atgcagcact cgcacgggct cgcgcacggg ccggccgagc
     1861 agctccgcga tccgttgctt ccgggccgca tcgccttcgg ccagcacgtt ggccgcccgc
     1921 ttcgtgcggt tgtagcacgc ggcacacggc gccacgagat cgaggcccat gtccgtcgcc
     1981 agaaggacat tccgggcccc cagcgcgtcc gccacttccc ggctcgcgct gtggcccgat
     2041 gtggccccgc agcagttcca gtcctcgagc tcggtcagct cgatgcccag ggcacggcac
     2101 acctcgaccg tcgaagcacc atattccttc gaggtgccgc tcagcg
//
LOCUS       DAOSPL010000885         2144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_51458_length_2144_cov_3.755864, whole genome shotgun sequence.
ACCESSION   DAOSPL010000885 DAOSPL010000000
VERSION     DAOSPL010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51458_length_2144_cov_3.755864"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /locus_tag="PLQ54_14830"
     CDS             complement(<1..1231)
                     /locus_tag="PLQ54_14830"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HOS94593.1"
                     /translation="MNEPPAIPSAHFEDPRRADQLLGRLARASSPALLREIEEPLFRA
                     LADSPDPDMALIGFERYCSARSDVMPLLALLARQPATLERLITVLGHSRMVADTLVRN
                     PEFLELLDPHELRPRTRDEMMASARASTRAFRTQPGQLDALRRFKRREMLRICAADVL
                     GLASLEEVTAQLSRLADACLEESLRLCWSVLETEVGPPVTAAGRPIPFCIISLGKLGG
                     GELNYASDIDITFVAGDYGTDGPGRPHAAAFFDRLASAVIQAMGQVTEEGFVFRVDCR
                     LRPEGSQGPLVRTLEGYRRYYAERGRPWERQALIKARYSAGDAALGAEFVRVLAPFVY
                     GGPPDPQALRSIRHNKQQIEHRVRAADRFYSHVKEGFGGIRDIEFAVQLTQLRLGHAR
                     REVQTGNTLAAIEALHRA"
     gene            complement(1280..1618)
                     /locus_tag="PLQ54_14835"
     CDS             complement(1280..1618)
                     /locus_tag="PLQ54_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOS94594.1"
                     /translation="MTRVEAIIRPLRLDEVRAALDEIGVRGMTVTEVRGYGKQRGHTE
                     RYRGTEYTITLLHKAKLEVVVPDDRVDEVVEAILASARTGEIGDGKIFLSPIAESIRI
                     RTGERGDDSL"
     gene            1772..>2144
                     /locus_tag="PLQ54_14840"
     CDS             1772..>2144
                     /locus_tag="PLQ54_14840"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94595.1"
                     /translation="MVPLEDALAQVDAVMASRPALPVEAVPLGKCVGRVLAEDVLARL
                     DVPPFDQSKMDGYAILADDRRGNYDLLEVVAAGRMPSAPLRPGACVKVMTGAPLPERA
                     GRVIVVENASEGSGSVTVHQHD"
BASE COUNT          319 a          717 c          754 g          354 t
ORIGIN      
        1 cggcgcggtg gagcgcctcg atggccgcca gggtgttccc cgtctgcacc tcgcgccggg
       61 cgtgcccgag acggagctgt gtgagttgga cggcgaactc gatgtcacgg atgccgccga
      121 agccctcctt gacgtgggag tagaaacggt ctgcggcgcg gacgcggtgt tcgatctgct
      181 gcttgttgtg ccggatggat cgcaaggcct gcgggtcggg agggcccccg tagacgaagg
      241 gggccagcac tcgcacgaac tcggcgccca gggcggcgtc gcccgccgag taccgggcct
      301 tgattagcgc ctgccgttcc cacggacgtc cccgctcagc atagtagcgc cggtaaccct
      361 cgagcgtgcg aaccagtggc ccctggctcc cttcgggtcg cagccggcaa tcgacccgga
      421 agacgaaacc ctcttcggta acctgtccca tggcctggat gacggcgctg gccaggcggt
      481 cgaagaacgc cgcggcgtgc ggcctcccgg ggccgtcggt gccgtagtcg ccggccacga
      541 atgtgatgtc gatgtcgctg gcatagttga gctcgccgcc gcccagcttc cccagcgaga
      601 tgatgcagaa cggtatgggt cgtcccgcgg ccgtgacggg cggtccgacc tcggtttcca
      661 gaacggacca gcagagccgc agggactcct cgaggcaggc gtcggcgagc cgcgagagct
      721 gcgcggttac ctcctcgagg ctcgccaggc ccagcacatc ggccgcgcag atgcggagca
      781 tctcccggcg cttgaaccgg cgaagcgcgt cgagctgccc gggctgagtt cggaacgccc
      841 gggtgcttgc ccgtgcgctg gccatcatct cgtcccgcgt gcgcggccgg agctcgtggg
      901 ggtcgagcag ctccagaaac tcggggttgc gcaccagtgt gtcggcaacc atacggctgt
      961 ggccgagcac cgtgatgagc cgttcgagtg tcgcgggctg ccgagcgagc aaggccagga
     1021 ggggcatgac atcacttcgg gccgagcagt agcgctcgaa cccgatcagc gccatgtcag
     1081 ggtcaggcga gtcggccagt gcccggaaca gaggctcctc gatctcgcgc aggagagcgg
     1141 gtgaagatgc ccgagccaag cggcccagca actggtcggc ccgccgggga tcctcgaagt
     1201 gggcgctcgg gatagccggt ggttcgttca tgggcccccg tccgaggtga agccaggtgg
     1261 accgcttgcc ctcgggctgc tagagggaat catcgccccg ctccccggtg cggatccgga
     1321 tcgactcggc gatggggctg aggaagatct tcccgtcacc gatctcaccc gtacgggccg
     1381 acgccaggat ggcttccacc acctcgtcca cgcggtcgtc gggcacgacg acctcaagct
     1441 tcgctttatg gaggagcgtg atggtgtact cggtcccacg gtagcgctcg gtgtggcctc
     1501 gttgtttgcc gtaaccccgg acctcggtca cggtcatgcc ccggacgccg atttcgtcca
     1561 gggcggcgcg cacctcgtcg agcctcagtg gccggatgat cgcttcgacc ctcgtcatcg
     1621 ctcgcactcc tcgcctcccg gaaccgccgc cgggagcgaa cctgtgctca gcatacccgt
     1681 gcgtcggcgc tgtagggaag agctgcgggt cggataggcg cgagccggcg cctcgacgaa
     1741 ctgctgcccg ggagtgaggt ggtctgccgc gatggtgcct ttggaggacg ctctggccca
     1801 agtggatgcc gtgatggctt ccaggccggc ccttccggtt gaggccgtgc ctctcggcaa
     1861 gtgcgtcggg cgtgtgctcg ccgaggacgt actggcgcgg ctcgacgtgc cccccttcga
     1921 tcaatcgaag atggacggct acgccatcct cgccgatgat cggcgcggca actatgacct
     1981 cctcgaggtt gtcgccgcgg gacgcatgcc aagcgctccg cttcgccccg gggcgtgtgt
     2041 gaaggtgatg acgggcgccc ccctgcccga gcgcgccggc cgcgtcatcg tggtcgagaa
     2101 cgcctccgag ggttctgggt ccgtcacggt tcaccaacac gacc
//
LOCUS       DAOSPL010000886         2142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_51518_length_2142_cov_4.943460, whole genome shotgun sequence.
ACCESSION   DAOSPL010000886 DAOSPL010000000
VERSION     DAOSPL010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51518_length_2142_cov_4.943460"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..1211
                     /locus_tag="PLQ54_14845"
     CDS             189..1211
                     /locus_tag="PLQ54_14845"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOS94596.1"
                     /translation="MLTLSVLMLAAPVGNETEAFQMVVAPVAGQNPRNSEAAIVPLND
                     GTLLLAWTEFYGGDGADHAPARIVGLTSSDGGRTWGGKYTLVENDGGCNVMEVNFLRL
                     KTGALALFHCQKNSEDKDCRVMMRTSTDEGRTFGPAKQLSPDGKYTGLTNGRSLRLQS
                     GRILLEAWEAGDSYCYLSDDDGETWREGGRVQPAGGPSYEPACIELKDGRVLMLMRTG
                     LGSQFASISADGGETWTTPKPTALEGTAAPVHLSRVPTTGDILAIWNHNPGAPSRNPL
                     TSAISKDEGETWEQFCNIEDAAGDAWAYPAVTWVGDRALVTYFNYAGGLSLWLRSIPA
                     GWFYGE"
     gene            1290..>2142
                     /locus_tag="PLQ54_14850"
     CDS             1290..>2142
                     /locus_tag="PLQ54_14850"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOS94597.1"
                     /translation="MDTRAMRVVARGVATRIPYLDRCLPRRSWGGFLGSYYYSVWLRH
                     LVLAGEAGLPTQYETVAELGPGDTLGVGMAALLSGAVSYCALDVMAFAANDRNLGLLD
                     EVRALFESRAGISGDFGPQVPSREFPSSLLPDSLLEKSLQPARVECIRRLLTGEAAQA
                     DDLVRVRYFAPWSDPAVIREASVDLVLSQAVMEYIDDLSFAYRAIHRWLRPGGFMSHM
                     IDLRCHGTASVWNGHWLYSDFVWNLIKGRRPCFISRHPWSIHEKAILDAGFRIIRTVK
                     NRADSTFG"
BASE COUNT          351 a          673 c          721 g          397 t
ORIGIN      
        1 gaagcccgcg tggttccccc catggcgccc ctccgcaacc aggctgatgg gccacgggag
       61 ccctaagtta ccgccaatgc ccctatccgg cccctccgaa gaccgggcgg atcgggcccg
      121 tcgaaggccc tcccgcctgg tcgactgaac ccctccacca atgcccaaac cggtgaggtg
      181 gaatggttat gctgaccctg tcggtgctca tgctcgccgc tcccgtgggg aatgagacgg
      241 aggcgtttca gatggtggtg gctccggtgg cgggccagaa cccgcgcaat agcgaggcgg
      301 ccattgtgcc gctgaacgat gggaccctgc tgctggcctg gacggagttc tatggtggcg
      361 atggggccga tcacgccccc gcgcggatcg tgggactcac ttcgtcggac ggcgggcgca
      421 catggggcgg caagtacacg ttggtcgaga acgatggcgg ctgcaatgtg atggaggtca
      481 acttcctccg gctcaagacc ggcgccctgg ccctcttcca ctgccagaag aacagcgagg
      541 acaaggactg ccgggtgatg atgcggactt cgacggacga ggggcgcacc ttcggcccgg
      601 ccaagcagct tagtcccgat gggaagtaca ccggcctgac gaacgggcgg agcctgcggc
      661 tccagtcggg ccggatcctg ctcgaggcct gggaggccgg cgacagctac tgctatctct
      721 ctgatgacga tggcgagacc tggcgcgagg gtgggcgcgt gcagccggcg ggcgggccga
      781 gctacgagcc ggcgtgcatc gagctcaagg acggccgcgt gctgatgctc atgcgcacgg
      841 ggctcggcag ccagttcgcc agcatctcag cggatggtgg cgagacgtgg acgaccccga
      901 aacccacggc gctcgagggc acggcggcac cggtgcacct cagccgcgtg cccaccacgg
      961 gcgacattct ggccatctgg aaccacaatc ccggcgcgcc ctcgcgcaac ccgctcactt
     1021 cggcgatctc gaaggacgag ggcgagactt gggagcagtt ctgtaatatt gaagatgcgg
     1081 cgggggatgc ctgggcctat ccggcggtga cctgggtggg cgatcgggcc ctggtcacct
     1141 acttcaacta cgcgggcggc ctgtcgctgt ggctgcggag catcccggcc ggttggttct
     1201 acggggaatg aggccgaccc ccgctcggat agggtccggc acttgtcatc ccatctccga
     1261 gatctcgccg caggaggtag gacagatgca tggatactag ggccatgcgg gttgtggctc
     1321 ggggagtagc cactcgcatc ccctacctcg accgctgtct tcctcggagg tcgtggggcg
     1381 gcttcctcgg cagctactac tactcagtct ggcttcggca tctggtccta gccggcgagg
     1441 ccgggttgcc gacgcagtat gagaccgtag ccgagctagg tcccggggat accctcggcg
     1501 tcggcatggc cgctctcctg tcgggcgctg tgtcttactg tgcgctggac gtgatggcct
     1561 ttgctgccaa tgaccgcaat ctgggcttgc tcgacgaagt gagggccttg ttcgagtctc
     1621 gagccggcat ctctggtgac ttcggccctc aggtaccgtc gcgcgagttt ccttcctctc
     1681 tgttgccgga cagcttactg gagaagtccc tacagcctgc ccgtgtcgag tgcattcgac
     1741 ggctcctgac gggagaagcg gcacaggctg atgatctggt ccgcgtgcgc tacttcgcgc
     1801 catggagcga tccggcagtg atccgagagg cctcggtcga tctcgtcctc tcgcaagcag
     1861 tgatggaata catcgacgat ctctctttcg cctatcgggc gatccacaga tggctccggc
     1921 cgggcgggtt catgtcgcac atgatcgact tgcgctgcca tgggaccgcg tcggtgtgga
     1981 atggccattg gctctactcg gacttcgtgt ggaacctcat caaggggcgc cggccgtgtt
     2041 tcatcagccg gcacccatgg tccattcatg agaaagccat tctggatgcg ggcttccgga
     2101 tcatacgcac cgtgaagaat cgagcggatt ccaccttcgg gc
//
LOCUS       DAOSPL010000887         2142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_51524_length_2142_cov_4.211787, whole genome shotgun sequence.
ACCESSION   DAOSPL010000887 DAOSPL010000000
VERSION     DAOSPL010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51524_length_2142_cov_4.211787"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PLQ54_14855"
     CDS             <1..949
                     /locus_tag="PLQ54_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94598.1"
                     /translation="AERVLRWCAGTQLETGHWYQRYWTTGLPASAWCVYPDFYQLDQT
                     AATVDAMCRLAPMLPEGAERDQYVAAVRPAVERGIAAIDRAVADDGLHERAADLWERF
                     RGVFSYTSAAVYRAWLSAKETYGLPASKAARVKRAVLERFWLEDERRFARGLDYDGNI
                     DRQHDSSVLGLIEPFGLLDLRDETEFAMAVGTLEAVERCLSLTIRGRRCILRHEHDPY
                     MGGAAGCVNTLWTARVHLAVAEAWLDRGRSDRALDHVGMARQHLSTAVACANPTGQLP
                     ELIVHMDSFTYWAAPHAWASGLFVVTVMKLERLRGLLSA"
     gene            complement(946..1800)
                     /locus_tag="PLQ54_14860"
     CDS             complement(946..1800)
                     /locus_tag="PLQ54_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOS94599.1"
                     /translation="MVEDTPQDEDLAHSEVARWDTGPPVAHPRPDWDLGRLLEEWKFE
                     PGELSARCFVGHDGRQKIQMRLELGILQMEADGRPDGQRPGGVESLFEYHQEALRTAG
                     PGATYRLTPDECLDLRHEALLYYHRYLCCFCIGDFERMERDTARNLAVFDFVRDHAEA
                     AYDRESMEPYRPYVLMMQARARAGMALRCGRYAQALSAVEQGIAAIQALGVSQLGQSE
                     PMGADEIEALQDLADEIRRSRPPTRLARLEAELQAAVECEDYMAAARLRDLIRMERSS
                     SEAPPSGR"
BASE COUNT          321 a          744 c          709 g          368 t
ORIGIN      
        1 ggccgagcgt gtcctgcgtt ggtgcgcggg tacgcagctc gagaccgggc actggtacca
       61 gcgttactgg acgacgggac tgcccgcctc ggcttggtgt gtctatcccg acttctacca
      121 gctcgatcag acggccgcca ccgtcgacgc catgtgccga ctcgccccca tgctcccgga
      181 gggtgccgag cgggatcagt acgtggcagc ggtgcgcccc gcggtcgagc gcgggatcgc
      241 cgccatcgac cgcgcggtcg cggatgatgg cctccacgag cgcgccgccg acctgtggga
      301 gcgtttccga ggcgtgttct cctacaccag cgccgcggtg taccgcgcat ggctgtccgc
      361 caaggagacc tacggactgc cggcttcgaa agccgcacgg gtcaagcgag cggtgctcga
      421 gcggttctgg ctcgaggacg aacggcggtt cgcccgcggg cttgactatg atgggaacat
      481 cgaccggcaa catgattcgt cggtactcgg cctgatcgag ccatttggcc ttctggacct
      541 gcgcgatgag accgagttcg ccatggccgt gggcaccctc gaagccgtcg agcggtgtct
      601 atcgctcacc atccggggcc ggcggtgcat cctgcgccac gaacacgatc cgtacatggg
      661 tggcgcggcg ggctgtgtca acacgctgtg gacggcccgg gtccacctcg cggtggccga
      721 agcttggctc gaccgtggtc ggtcggaccg agctctcgac catgtcggga tggcccgtca
      781 gcacctgagc acagccgtgg cctgcgccaa tcccacgggc cagctccccg agctcatcgt
      841 gcacatggat tcgttcacct actgggcggc gccccacgcg tgggcttcgg ggctcttcgt
      901 ggtcactgtc atgaagctcg aacggttgcg cggacttctg tcggcctagc gcccggatgg
      961 cggcgcctcc gaactcgaac gctccatgcg gatcagatcg cgcagccggg cggcggccat
     1021 gtagtcctcg cactccacgg ccgcctgcag ctcggcctcg agccgcgcca gacgggtcgg
     1081 tggtctgctc cggcggatct cgtcggccag gtcctgcagc gcttcgatct cgtcggcgcc
     1141 catcggttcc gactgcccga gctgtgatac gcccagggcc tggatggccg cgatgccctg
     1201 ctccacagcg gagagggcct gcgcatagcg accacaccgc agagccattc ccgcccgggc
     1261 ccgcgcctgc atcatcaaca catacggccg gtagggctcc attgactcac ggtcataggc
     1321 ggcctcggcg tggtcacgga cgaagtcgaa caccgccagg ttccgcgcgg tgtcgcgctc
     1381 catcctctcg aagtccccaa tgcagaagca gcagaggtac cggtggtagt acagcaacgc
     1441 ctcgtggcgc aggtccaagc actcatccgg agtgagacgg tatgtggctc cgggtcccgc
     1501 cgtccgtagg gcctcctgat ggtattcgaa caacgactcg acacccccgg ggcgctgtcc
     1561 gtcgggccgt ccgtcggcct ccatctgaag gattcccagc tccagacgca tttggatctt
     1621 ctgacgcccg tcgtgtccca cgaagcagcg agccgacagc tccccgggct cgaacttcca
     1681 ctcctccagc aggcgtccga ggtcccagtc cgggcgcgga tgcgccaccg gaggtccggt
     1741 gtcccaccgg gcaacctcgc tgtgcgccaa gtcctcgtcc tgcggagtgt cctccaccat
     1801 tccaccccct gcccgatgat atagcgggga tgcccgccca cgttgcgcgg catagtaggg
     1861 ttccccacac cggcctctgc cccttcccat gcctcggccg agcgaggact ggggcaatga
     1921 ccggcgaaga aggaaagcca ccgcctgttg gcgggtgggg cggtgctccg ggggtaccca
     1981 agtgcagcga tctggtcgag ttgtgatctt catgtggctc cgggcggcga cccggaggtg
     2041 atgcctctcc tggccgggct ggtgggcgcc atcgcgctcc agttctgctg gtaccgagct
     2101 cgccacgaac tgcgacgtgc cggggatgcg ctctcgaggg ga
//
LOCUS       DAOSPL010000888         2139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_51626_length_2139_cov_3.617083, whole genome shotgun sequence.
ACCESSION   DAOSPL010000888 DAOSPL010000000
VERSION     DAOSPL010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51626_length_2139_cov_3.617083"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="PLQ54_14865"
     CDS             <1..804
                     /locus_tag="PLQ54_14865"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HOS94600.1"
                     /translation="PVVVAAAAAALAANLLQVGFLCAPKVIRLDASRLNPVNGFKRLF
                     SLRACVELLKALLKIAVVAYIGYSTLWGSRHLLFVLPEQTVAGVMLIIGDIAYRICLK
                     AGLALLVLALADYGFQRWQHEKDLRMTRQELRDEYKRQEGDPLLKQRIRQRQRELARR
                     RMMAEVPKADVVITNPTHLAVALRYDPAHMDAPCVVAKGERALAERIREIAREAGVPV
                     IENRELAQSLFRLVPVGGVIPSQLFEAVAQILAYVYRIRGKTPAFSSAA"
     gene            938..>2139
                     /locus_tag="PLQ54_14870"
     CDS             938..>2139
                     /locus_tag="PLQ54_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOS94601.1"
                     /translation="MEAILAPARLMSRYRDLQVPVALIAVIAMLVLPVPPVLLDALLS
                     INLVLAITILLLTTYTKDALEFSVFPSLLLFTTLFRLALNVAAMKLILTTAHAGKVIY
                     EFGDLVVGGNQVVGIILFATLVVIQFIVITSGAGRVAEVAARFTLDAMPGKQMSIDAD
                     LNAGIIDEAEARRRRRNIEREADFYGAMDGATKFVRGDAIFAIIAVLLNIVGGFIIGV
                     MMKHMTAAEAFETYTRLTVGDGLVTQIPALIVSTATGIIVTRAASEENLGSDMATQLT
                     RYPREIMVAAVALLIFGAVLQAVPMITVSLLTGLGAYALTATAKRERVAAVRAEEEKA
                     KAAPRGPEDVTPLLEVEPIELEIGYGLIPLAHPGQGGDLLDRVTMIRKQAAIDLGLVV
                     PPIRIRDN"
BASE COUNT          347 a          689 c          695 g          408 t
ORIGIN      
        1 ccggtcgtgg ttgccgctgc tgccgctgcg ctggctgcga atctgttgca ggtggggttc
       61 ctgtgcgccc ccaaggtcat tcggctggac gccagtcgcc tcaatcccgt caacggcttc
      121 aagcgcctgt tctcgctgcg ggcgtgtgtc gagctgctca aggcgttact caagattgcg
      181 gtggttgcgt atatcggtta cagcacgctc tgggggagcc gccatctgct gttcgtgctg
      241 cccgagcaga cagtggccgg cgtcatgctg atcatcggcg acatcgcgta ccgtatctgc
      301 ctcaaagcgg ggctcgcgct gctggtgctc gcgctggccg actacgggtt ccagcgatgg
      361 cagcatgaga aggatctccg gatgacgcgt caggagctcc gcgacgagta caagcggcag
      421 gagggtgacc cgctgctgaa acagcgaatc cgccagcggc agcgcgaact cgcccgccgg
      481 cggatgatgg ccgaggtgcc caaggccgac gtcgtcatca ccaacccgac gcacttggcc
      541 gttgcgctaa ggtacgaccc ggcgcatatg gacgcgccct gcgtggtggc gaagggcgaa
      601 cgcgcactgg ccgagcggat tcgcgagatt gcgcgcgagg cgggcgtgcc cgtgatcgag
      661 aacagggagt tggctcagtc gctgttccgt ctggtgcccg tgggaggcgt gataccgagc
      721 cagctcttcg aggccgtggc acagatcctg gcttacgtct atcggatccg cggcaagaca
      781 ccggccttct cgtcggccgc gtaggcccgg tctgtggacg gagacggggc agggggcgag
      841 ggggctctgt ccttccccgc gtgtggggag aaccgggact gggcactgat atcgtgactt
      901 ttgacttctg attgctactt ccaagaggcc gctcagcatg gaagccatcc tcgcaccggc
      961 acggctcatg agccgctacc gggacctcca ggtcccggta gcactcattg ccgtcatcgc
     1021 catgctcgtg ctgcccgtgc cacccgttct cctcgatgcc ctgctgtcga tcaacctcgt
     1081 tctggccatc accattttgc tgctgacgac ctacaccaag gacgccctcg agttctcggt
     1141 gttcccgtcg ctgttgctgt tcaccaccct gttccgtctg gccctgaacg tagctgccat
     1201 gaagctgatc ctgaccacgg cccacgcggg gaaggtgatc tacgagttcg gcgacctggt
     1261 ggtcggaggc aaccaggtcg tgggcatcat cctgttcgcg acgctggtcg tcattcagtt
     1321 tattgtcatc acatcgggcg ctgggcgcgt ggccgaggtg gctgcacgtt tcacgttgga
     1381 cgccatgcct ggcaagcaga tgtccatcga tgccgacctg aacgctggga tcatcgacga
     1441 ggctgaggcc cggagacgcc gccgcaacat cgagcgggag gcggacttct acggagcgat
     1501 ggacggcgcg acgaagttcg tccgtggtga tgcgatcttt gccatcatcg ccgtgctcct
     1561 gaacatcgtg ggcgggttca tcatcggcgt gatgatgaag cacatgaccg ccgctgaggc
     1621 attcgagacc tacacccgcc tcacggtcgg cgacggactc gtcactcaga tccctgcgct
     1681 catcgtctcc accgccaccg gcatcatcgt cacgagggcc gccagcgagg agaacctggg
     1741 ctcggacatg gccacccagc tcactcgcta cccccgggag atcatggtgg ccgccgtagc
     1801 tctcctcata ttcggggccg tgctgcaggc cgttcccatg atcaccgtct cgctcctgac
     1861 cggcttgggg gcctatgcgc tgacggccac cgccaaacgc gagcgggtcg ccgcggttcg
     1921 ggctgaggaa gagaaggcca aggcagcgcc ccggggtccg gaggacgtta ccccgctgct
     1981 cgaggtcgag cccatcgagc tcgagatcgg ctatggcctg attccgctcg cccatccggg
     2041 ccagggcggc gatctgctcg accgtgtgac gatgatccgc aagcaggcgg ccatcgacct
     2101 tgggctcgtg gtgcccccaa tccgcatccg tgacaatat
//
LOCUS       DAOSPL010000889         2139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_51634_length_2139_cov_3.030710, whole genome shotgun sequence.
ACCESSION   DAOSPL010000889 DAOSPL010000000
VERSION     DAOSPL010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51634_length_2139_cov_3.030710"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /gene="serA"
                     /locus_tag="PLQ54_14875"
     CDS             complement(<1..1155)
                     /gene="serA"
                     /locus_tag="PLQ54_14875"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOS94602.1"
                     /translation="MSKILVCDSIAEEGVAKLREAGSVHVSTGLSEEQLIEAARDACA
                     IMVRSSTYITAPVIRAAPELRVVGRAGVGVDNIDVDAATAAGVVVVNSPSGNTVAAAE
                     HTIAMMMAVARRIPFAHSRMLARDWAKSKCLGTEVRGKILGVVGLGKIGTEVARRAKG
                     LEMQVIAHDPMLSEDHMQKMGVEPVSLDDLLARSDFVTLHVPGGAKTAQLINEDALAK
                     MKPAAFLINCSRGAVLDEAAVARALSEGRLAGAAIDVFQGEPNPWSSPLLDAPNVVLT
                     PHLGASTVEAQLVASLDVAEQICDVLAGRQARSAVNLPRVPAETAEELRPWMGLAERV
                     GMLVANLAQSGLRSAQVEYLGELRAFDCSLLTRAVLVGLLSRVGTDNLNII"
     gene            complement(1183..>2139)
                     /locus_tag="PLQ54_14880"
     CDS             complement(1183..>2139)
                     /locus_tag="PLQ54_14880"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOS94603.1"
                     /translation="GGMEAAIANTLSPGDAVLCISVGVFGDRFAKIAQAFGMDVDVLA
                     FEWGQAADPSAIEQKLKSGKQYKAVTLTHNETSTAITNDTASIAPMVREHGALFLVDC
                     ISGMLCTDFRTDEWDCDVVVAASQKAFMLPPGLCFVSVSPRAWEAVERAKSPRFYFDF
                     RAMREPAKKGQTAYTPAVGLFFGADAALGMILREGAEQVIARHARLAAGVRAGVKALG
                     LKLFSDEAHASNGVTAIVPPAGIDADSLRALLSQRYSVVLSGGQGKLKGQIFRIAHMG
                     AVSEKDLLGALGTLELALSEMGHRAEPGASVAAAVRAWTISH"
BASE COUNT          371 a          739 c          678 g          351 t
ORIGIN      
        1 gatgatgttc aggttgtcag tgccgacgcg gctcagcagg ccaaccagaa ccgcccgagt
       61 cagcagcgag cagtcgaagg cacgcagctc gcccaggtac tcgacctgag cacttcgcag
      121 acccgattgc gccaggttgg caacgagcat gccgacgcgc tcggccagac ccatccaggg
      181 ccggagctcc tcggcagtct cggcgggcac tcggggcagg ttcaccgcgc tccgggcctg
      241 cctgccggcc agcacgtcgc agatctgctc ggccacgtcg agactcgcca cgagctgggc
      301 ctcgacggtc gaggcaccca ggtgtggtgt cagaaccaca ttgggggcgt ccagcaacgg
      361 gctcgaccat gggttgggtt cgccctggaa cacatcgatg gcggcaccgg cgagccggcc
      421 ctcactgagc gcgcgggcga cggctgcctc atcgagaacc gcgccgcgac tgcagttgat
      481 gaggaacgcc gccggcttca tcttcgccaa cgcgtcctcg ttgatgagct gagcggtctt
      541 ggccccgccg ggcacatgga gcgtgacaaa gtcgctgcgg gcgagaaggt catccagcga
      601 gaccggctcg acgcccatct tctgcatgtg gtcctcggag agcatgggat cgtgagcgat
      661 gacttgcatt tcgaggccct tggcgcgccg cgccacctcg gtgccgatct tgcccaagcc
      721 gacgacaccc aggatcttgc cgcgcacctc agttcccagg cacttgctct tcgcccagtc
      781 gcgggcgagc atgcgactgt gagcgaaggg gatcctccga gccacggcca tcatcatggc
      841 aatggtgtgc tcggctgcgg ccacggtgtt gccgctgggc gagttaacga ccaccacacc
      901 cgcggcggtt gcggcatcca cgtcgatatt gtcgacgccg acgcccgccc gcccgaccac
      961 ccggagctcg ggggcggccc ggatgaccgg agcagtgatg taagtggagc tgcgcaccat
     1021 gatggcgcaa gcgtctcgag ccgcctcgat cagttgctcc tcactcaggc ctgtcgagac
     1081 gtggaccgaa cccgcctccc gcagctttgc aacgccctct tcggcgatgg aatcacatac
     1141 gaggatcttg ctcacacttg cccgccttcc cgtgcgcccg tcctaatgcg agatggtcca
     1201 tgcccggact gccgcagcca ctgaagcacc cggctcggcc cgatgcccca tctcgctcag
     1261 agccagttcg agagtgccca gcgccccgag caagtccttc tccgagaccg cgcccatatg
     1321 ggcgatgcgg aagatctggc ccttgagctt gccctgtccg ccgctcagca ccaccgagta
     1381 gcgctgggag agcagtgccc gaagtgagtc tgcatcgata cccgccgggg gcacgatggc
     1441 cgtcacgccg ttggaggcgt gagcctcgtc ggagaagagc ttgaggccca gcgccttcac
     1501 cccggcgcgg acgccggcgg ccagccgtgc gtgccgcgcg atcacctgct cggccccctc
     1561 ccgcaggatc atgcccagag cggcgtcggc cccaaagaag agccccaccg ccggcgtata
     1621 ggccgtctgc cccttcttgg cgggttcgcg catggcgcgg aagtcgaagt agaagcgtgg
     1681 gctcttggcg cgttcgaccg cctcccaggc cctggggctg acactcacga aacagagacc
     1741 cggaggcagc atgaacgcct tctgggaagc tgctacgacc acatcgcagt cccactcgtc
     1801 tgtgcggaag tccgtgcaca gcatgcccga gatgcagtca acgagaaaca aggcgccgtg
     1861 ttcacgaacc atcggcgcta tgctggccgt gtcgttggtg atggccgttg atgtctcgtt
     1921 gtgcgtgagt gtgacggcct tgtactgctt cccagacttc agtttctgct caatagcgga
     1981 tgggtccgcc gcctgtcccc actcgaacgc gaggacatcg acgtccatgc caaaagcctg
     2041 ggcgatcttc gcgaagcggt cgccgaacac accgaccgag atgcacagca cggcgtcacc
     2101 gggcgacagc gtgttcgcga tggccgcctc cataccgcc
//
LOCUS       DAOSPL010000890         2138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_51663_length_2138_cov_3.378781, whole genome shotgun sequence.
ACCESSION   DAOSPL010000890 DAOSPL010000000
VERSION     DAOSPL010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51663_length_2138_cov_3.378781"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2003
                     /locus_tag="PLQ54_14885"
     CDS             <1..2003
                     /locus_tag="PLQ54_14885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOS94604.1"
                     /translation="MARELSELRGERLRLETVLDGFPAWVCILDAEYSITFTNIMFRD
                     TFGPTEGRKCYEVLAAQAQRCEQCPLQEVGKGLAAGRFHETTLADGRRYQVYAYPFQD
                     ECGAPRVLEMGVDVTASREAAEALERSEVLFRAVVNSSVDAMVAIDAQSRVRIFNPAA
                     ERMLGWQAEQMLGQPVDVIMPERFRGDHRSMVASYFATGQPCGAVGRTVSLPALRSDG
                     TELPVELTLSHRFDAEDPIVLAVMRDTTERERALEALRESEARLRAIYEGSSDAIMLL
                     SEDHFIDCNPRTLAMFAVEDRARLVQMRPTDLLPPVQPDGRPSWQVAREHIRTAYEQG
                     HHRFEWIHRRTTGEDFAAEVILSAFELGGRTVLQATVRDISERREAEQRLRRAQKLEA
                     IGQLAAGIAHEINTPAQYVGDNTRFLQESLCGLIELITSYDTLLTQIKSGAPVDPELV
                     QDIETAREIADIEYLCEEIPNAIKQSLEGIDRISTIVRAMKDFSHPGVDERTPIDLNR
                     CVESTITVARNEWKYVAELDAELDRSLPLVPCFPADINQVVLNLIVNAAHAVAEAQEQ
                     GAPRPGRIRVSTHRAGDWAEIRVDDSGPGIPEAIRDRIFEPFFTTKAVGKGTGQGLSL
                     AHSTITQKHGGELLFETCMGQGTTFIVRLPLATPSAEGGDAR"
     gene            2000..>2138
                     /locus_tag="PLQ54_14890"
     CDS             2000..>2138
                     /locus_tag="PLQ54_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009182431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HOS94605.1"
                     /translation="MKLRVLFVDDEPNVLQGLRRMLRPQRDEWDMDFSSSGAAALAAM
                     RE"
BASE COUNT          418 a          684 c          682 g          354 t
ORIGIN      
        1 gaatggcccg cgagctatcc gagctccgag gtgagcggct gcgcctcgag acggtgctcg
       61 acggattccc cgcgtgggtg tgcatcctcg atgccgaata tagtattaca ttcaccaata
      121 tcatgttccg ggacacgttc ggcccgactg agggacgcaa gtgctacgaa gtgctggcag
      181 cgcaagcgca acgatgcgag cagtgccctc tacaagaggt cggcaagggc ctggccgccg
      241 gcaggtttca cgagacgacc ctcgctgacg gccgacggta ccaggtctac gcctacccat
      301 tccaggacga gtgcggcgcg cctcgggtgc ttgagatggg cgtcgacgtg accgcctccc
      361 gcgaggcagc ggaagccctg gagcggagcg aagtcctctt ccgggccgta gtcaactcga
      421 gcgttgacgc catggtggcc atcgacgccc agagccgggt acgcatcttc aacccggcag
      481 cggagcgtat gctgggctgg caggccgagc aaatgctcgg gcagcccgtg gacgtcatca
      541 tgcccgagcg attccgtggt gaccacagga gcatggtggc cagctacttc gcgaccggtc
      601 agccgtgcgg cgcagttggc cgcacggtct cgcttccggc gctgcggtcc gacggaaccg
      661 aactgcccgt ggaactgacc ctctcgcacc gcttcgatgc cgaggacccg atcgtgctgg
      721 ccgtgatgcg ggacaccacc gagcgcgaac gcgctctgga ggccttgcgt gagagcgaag
      781 ctcgcctccg cgccatctac gagggatcca gcgacgccat catgctcctc agcgaggacc
      841 acttcataga ctgcaatcct cgaacgctgg ccatgttcgc ggtggaggac cgggctcggc
      901 ttgtccagat gcgtccaact gacctgttgc cgccggtaca gccggacggt cggccatcct
      961 ggcaggtcgc acgagagcac atcaggacag catatgagca ggggcaccac cgtttcgagt
     1021 ggatacaccg caggacgacg ggcgaggact tcgcggctga agtcatcctt tcggccttcg
     1081 aactcggggg gcgcactgtg ctgcaagcca cggtgcggga catcagcgag cgaagagagg
     1141 cggagcagcg cctgcgccgt gcccagaagc tcgaggccat cggacaactc gccgcgggca
     1201 ttgcccacga gatcaacaca cccgctcagt atgtcggcga caacactcgc ttccttcagg
     1261 agtctctatg tgggctcatt gagctgataa caagctatga tactctcctc acccagatca
     1321 agtcgggcgc gcccgtagat cccgagctgg tgcaagacat cgagacggca cgagagattg
     1381 ccgacatcga gtacctctgt gaggagatcc ccaacgccat caaacagtca ctcgagggca
     1441 tcgaccgcat ctccacgatc gtccgcgcca tgaaggattt ctctcacccc ggcgtcgacg
     1501 agcgaacgcc tatcgatctg aaccgatgtg tcgagagcac catcaccgtg gcccggaatg
     1561 agtggaagta cgtcgccgag cttgatgcgg agctggaccg gtctcttccc ctcgtaccat
     1621 gcttccctgc cgatatcaac caagttgtgc tcaacctcat cgtgaatgcg gcccacgcgg
     1681 tggccgaggc ccaggagcag ggcgctcctc gacccggccg gatacgagta tcgacacacc
     1741 gagcgggaga ctgggcggag atccgggtgg acgactcggg accaggcatc cccgaggcga
     1801 tccgcgaccg catcttcgag cctttcttca cgaccaaagc ggtcggcaag ggtacgggtc
     1861 agggactgtc gctcgcccac tcgacgatca cccagaaaca cggtggagag ctgctcttcg
     1921 agacatgcat gggccagggc acgaccttca tcgtccgtct gcctctggcg acaccgagcg
     1981 cggagggcgg agatgcgaga tgaagctgcg cgtgctgttc gtcgatgacg aacccaacgt
     2041 gctacagggt ctccgccgga tgctgcgccc tcaacgcgat gagtgggaca tggacttctc
     2101 gagcagcggc gcggccgcgc tggctgccat gagggagt
//
LOCUS       DAOSPL010000891         2134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_51779_length_2134_cov_3.293891, whole genome shotgun sequence.
ACCESSION   DAOSPL010000891 DAOSPL010000000
VERSION     DAOSPL010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51779_length_2134_cov_3.293891"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="PLQ54_14895"
     CDS             <1..676
                     /locus_tag="PLQ54_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94606.1"
                     /translation="VQPPSAGRRWQIGLAAALILIAMGGPAAWHFGARARNTGAPSPP
                     EPAKAEGAPATPRAPGMGAALTERPEAEAPSPPAPARNGDAGAYAFELAPNADGRGVT
                     ASLNDGAPGPVDVVLQRFRQNAIEEVARQRLKPGERVALPLPRDAYPTSLTQKQGIRL
                     CFTVDSSGDLAWAPSGKIVLPKSGSVSIEDSKIYLVHRLSPIILAVKALPSPGQGPSV
                     DDPLTF"
     gene            726..938
                     /locus_tag="PLQ54_14900"
     CDS             726..938
                     /locus_tag="PLQ54_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94607.1"
                     /translation="MGAKAAQLPLPDEAVLYFEADASEGKATGGHEGCVFRDGKASLC
                     FGDGRVKLLSEDETAKRIWEPGKKGK"
     gene            1420..>1890
                     /locus_tag="PLQ54_14905"
     CDS             1420..>1890
                     /locus_tag="PLQ54_14905"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOS94608.1"
                     /translation="MQTAKVNSRWAGVLAVAGLALTVSPSRADDPVRFVGGDAANLRS
                     APSLTASVQCTLSPGTELRLKGQQGEWRQVYVPSKQTTGWIVERLLSDTPPAGQARDV
                     KYVRGDDVNIRSGPGQRFDRVGILPKGTRVDVVAYEGQWRKIRVPETGEIGWVAD"
     assembly_gap    1891..1900
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1901..>2134
                     /locus_tag="PLQ54_14910"
     CDS             <1901..>2134
                     /locus_tag="PLQ54_14910"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOS94609.1"
                     /translation="SGGDDDLNDDAPAAAAGQVRYAGGDDLCLRSGPGGQHQVLCSLA
                     LGTRFQVIEVGSEWCKVRLDSGTTGYVSRQYVT"
BASE COUNT          354 a          705 c          776 g          289 t
ORIGIN      
        1 ggtccagccg cccagtgccg gccgccgatg gcagatcggc ctcgccgcgg cgctgatcct
       61 catcgccatg ggcggccccg cggcgtggca cttcggcgca agggcacgaa ataccggcgc
      121 ccccagcccg cccgagccag ccaaggccga gggcgctccg gccacacccc gggcgccagg
      181 catgggcgcc gcgctcacag aacggcccga ggcggaggca ccatcgcctc ccgccccggc
      241 gcgcaacgga gacgcgggcg catacgcatt cgagctggcc ccgaatgcgg acggtcgcgg
      301 cgtcactgcg tcgctcaacg acggcgcacc gggcccagtc gatgtcgtcc tgcagcggtt
      361 ccggcagaat gccattgagg aggtggcccg gcagagactg aagccggggg agagggtggc
      421 gttacctctg cccagggacg cctaccccac ctcgctcacg cagaagcagg gcatccggct
      481 gtgcttcact gtcgactcgt ccggggacct ggcttgggca ccgtcgggga agatcgtgct
      541 gcccaagtcg ggctccgtga gcatcgagga ctcgaagatc tacctcgtgc atcgactgtc
      601 accgatcatc ctggcagtga aggctctccc cagccccggc caggggccca gtgtagacga
      661 cccgttgacg ttctagccga acggatccca cggtgggccg cgggggcagc gcgcggtggc
      721 acgcggtggg ggccaaggcc gcccaactcc cgctcccgga cgaggccgtg ctctacttcg
      781 aggccgatgc gagcgagggg aaagcgacag gcgggcacga gggttgcgtg ttccgcgacg
      841 gcaaggcgag cctctgcttc ggcgacgggc gtgtgaagct cctcagcgag gacgagacgg
      901 ccaagcggat ctgggagccg ggcaagaagg gcaagtaggg agggcgcggc ggcaggcttg
      961 cgatggaagc cgctagccgg cccggtccca cggcccccac tacgcctggc tcgacacggc
     1021 tgcgagaggg ctggagctcg gcggcgagag cgagacgggg cggaagtgta cgccgccgag
     1081 cggcatgcag ccaggagatg cactccccgc cacccacagc acgacggccg accagagacc
     1141 cgccagagct cgatgcatga ggaggcttga tggtcggcac aaccaaggga tcccaatggg
     1201 cctccggtcc tgccggcgaa cgggccttcg tgtctggctc gcagagggaa acctccacgc
     1261 cgatgaggtg tccaaacact gctccgcatc gccgcgtggg ccgcgtgagg cattgacggc
     1321 cgtggggtcc gatggtaccg tgtcggggtc gcgtgtgaac ctgccgagtg gccctcccgc
     1381 tatgagacgg gggcggttcg gcgccgcgac gggagcgcta tgcagacagc gaaggtgaac
     1441 tctcgatggg cgggggtcct ggcagttgca gggctcgcgc tcaccgtgtc gccgtccagg
     1501 gctgatgatc ctgttcgatt cgtgggcgga gacgcggcca acctgcgatc ggcgccgagc
     1561 ctgaccgcct cagttcagtg cacgctcagt ccggggactg agctgcggct caagggccaa
     1621 cagggcgagt ggcgtcaggt ctacgtcccc tcaaaacaga ccaccggctg gatcgtcgag
     1681 cgcttgcttt cggacacgcc acccgcgggc caggcacggg acgtcaagta cgttcgcggc
     1741 gatgacgtga acatccggag cggccccgga cagcgcttcg accgtgtcgg catcctgccc
     1801 aagggaactc gtgtcgacgt ggtggcctac gagggtcagt ggcgcaagat ccgcgttccg
     1861 gagaccggcg agatcggctg ggttgccgac nnnnnnnnnn gagcggcggc gacgatgacc
     1921 tcaacgacga cgcccccgcc gccgcggcgg gccaggtgcg ctatgcgggc ggggacgatc
     1981 tgtgcctccg cagcggaccg ggcggccaac accaggtgct ttgctcgctc gcgctgggca
     2041 cgcgcttcca ggtgatcgag gtggggagcg agtggtgtaa ggtccggctc gactccggaa
     2101 ccaccgggta cgtgtcgcga cagtacgtga ccga
//
LOCUS       DAOSPL010000892         2132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_51835_length_2132_cov_3.288397, whole genome shotgun sequence.
ACCESSION   DAOSPL010000892 DAOSPL010000000
VERSION     DAOSPL010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51835_length_2132_cov_3.288397"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PLQ54_14915"
     CDS             <1..656
                     /locus_tag="PLQ54_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94610.1"
                     /translation="GNVLQLTENTALDGRPRWSPDGSTIVYHSTVDNQWLELYTIPAT
                     GGEPTRLTTNDYPDSSPVFSPDGASIAWSARPVSGQPAEIWVMDLATRTPRSLTAATH
                     SANNESALFPAYAPVDGNHPEDPVRIAYSVQPPDTNTDLWVMNADGTGAYRVTDTHGG
                     ATRPAWSADGQFIVFDMFSSGITTLYIVSAGGGAPAELLSKRQTEGSDTSPFFYPIP"
     gene            786..>2132
                     /locus_tag="PLQ54_14920"
     CDS             786..>2132
                     /locus_tag="PLQ54_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS94611.1"
                     /translation="MARGRRREWGEADDDTPELVMGEDGPGDESLGLETQVQAAAIVD
                     ELLRATDEKDPRWGALFLLRHQLRVDERQMDEARQAIAELQQAYDKLTSPANRLGTFL
                     DAPEEGIARVAVGDTEYYANVDSQKVPETPVPGACVRLNDAFAVVGDLGPWTSGPLTK
                     VAEVLADGRLRIGTEPGGLGSRIVVRSSALDGIALRQGDEVRLDPSGRVAVEHFHRAE
                     TRDYFFEEVPETPWSAVGGQEDAIRLIRDTIELPLLHPELYERFHKRPVKGILLYGPP
                     GCGKTLIGRATAYNLTREYRQRFGRDVKECFMYISGPRILNMWLGESERMVREIFAAA
                     RERAAEGHLVFVFVDEAESLLRTRSAGRTLNIANTLVPQFSAEMDGMVSLQNVVVILT
                     SNRPDYIDPAILRPERIDRKIKIGRPDLSAAREIFRIYLDDRVPLSAELVAEHGSDP"
BASE COUNT          347 a          634 c          780 g          371 t
ORIGIN      
        1 gggggaacgt gctgcagctc accgagaaca cggccctgga tggtcgtcct cggtggtcac
       61 ccgacggatc gaccatcgtg taccactcga cggtggataa ccagtggctc gagctgtata
      121 cgattccggc cacgggcggc gagccgactc gcctcacgac caacgactat ccggatagca
      181 gcccggtgtt cagccccgat ggtgcgtcga ttgcctggtc ggcccggccg gtgagcggcc
      241 agccggccga gatctgggtg atggatctgg cgacgcggac gcctcggtcg ctcacggcag
      301 cgacccactc ggccaacaac gagtcggccc tgttcccagc ctatgcaccg gtcgacggga
      361 atcatccgga ggatccggtg cggattgcgt actcggtgca gcccccggat accaacacgg
      421 acctgtgggt tatgaatgct gatggcacgg gtgcctaccg ggtaacggac acccacggcg
      481 gggccactcg gccggcgtgg tcggccgacg ggcagttcat cgtgttcgat atgttcagtt
      541 cgggcatcac gacgctgtac atcgtgagcg caggcggcgg ggcgccggcg gagctgctct
      601 ccaagcggca gacggagggg agcgatacga gcccgttctt ctacccgatc ccttagcgcc
      661 ggggccgttg cgccggcctg ggttcgggcg acgcgggcga atccgccgct cctcgggtgt
      721 tgaacagcgt ggaggcgggg gcagagggcc gtgctcgttc cgggcggccg gccccgggga
      781 tgaccatggc acgaggcagg cggcgcgagt ggggcgaggc ggatgatgac acgcctgagc
      841 tcgtgatggg cgaggacggg ccgggcgatg agtctcttgg cctcgagacc caggttcaag
      901 ccgcggcgat tgtcgatgag ctgctgaggg cgacggacga gaaggacccg cgctgggggg
      961 cattgttcct ccttcggcac caactgcgtg tcgatgagcg gcagatggat gaggctcggc
     1021 aggccattgc cgagctccaa caggcgtatg acaagctgac cagtcccgcc aatcgcctgg
     1081 gtacgttttt ggatgcaccc gaggaaggca ttgcgcgtgt ggcggttggc gacaccgagt
     1141 attacgccaa cgtggattct cagaaggtgc cggagacgcc ggttccgggc gcgtgtgttc
     1201 gcctcaacga tgcgtttgcg gtggtcggcg acctgggtcc atggaccagt gggccgctga
     1261 cgaaggtggc cgaagtcctt gccgacgggc ggctgcggat tgggacggag ccgggcgggc
     1321 tgggcagccg gatcgtggtt cggtcgtcgg ctctggacgg cattgctttg cggcagggcg
     1381 atgaggttcg gctcgacccc agcgggcggg tggccgtcga gcatttccac cgggcagaga
     1441 cgcgggacta ctttttcgaa gaggtgccgg agacgccgtg gtcggccgtc gggggccagg
     1501 aggatgccat ccggctgatc cgggacacca tcgagctgcc tctcctgcac ccggagctgt
     1561 atgagcggtt ccacaagcgt ccggtcaagg gcatcctgct gtatgggcct cccggttgtg
     1621 ggaagacgct gatcggacgg gccacggcgt acaacctcac ccgggagtac cggcagcgtt
     1681 tcgggcgcga cgtcaaagag tgcttcatgt acatcagtgg gccccggatc ctcaacatgt
     1741 ggcttgggga gagcgagcgg atggtccgcg agatcttcgc tgcggcgcgg gagcgtgcgg
     1801 cggagggcca cctggtattc gtctttgtcg acgaagccga gtcgttgctc cgcacccgga
     1861 gtgcggggcg cactctcaac atcgcgaaca ccctggtgcc ccagttctca gcggagatgg
     1921 acgggatggt gtcgctgcag aacgttgtgg tgatcctgac gtccaatcgg cccgactaca
     1981 tcgatccggc gattctgagg cccgagcgca ttgaccggaa gatcaagatc ggccggccgg
     2041 acctgtccgc ggcgcgtgag atctttcgca tctacctcga tgatcgggtg cccctgagtg
     2101 ccgagctcgt ggccgagcat ggcagtgatc cg
//
LOCUS       DAOSPL010000893         2130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_51911_length_2130_cov_2.557108, whole genome shotgun sequence.
ACCESSION   DAOSPL010000893 DAOSPL010000000
VERSION     DAOSPL010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51911_length_2130_cov_2.557108"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1623)
                     /locus_tag="PLQ54_14925"
     CDS             complement(97..1623)
                     /locus_tag="PLQ54_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOS94612.1"
                     /translation="MARVVVVLSALVLIGAAAGGAGAADLVRNGTFSDIALGNLPHQW
                     TVPTGTERYAAAVDDDGHSGKRSLRVRLAAGAVVAPVAQVVMCEAQTDYVLSAWFKAD
                     ALRPAVTVSGVLESGEAVELARALSAGPGGWQGASVRFNSGPLSELTVSLLPHAGDGG
                     PAEGTSWFDDVQIVPAAEYRPEGGRVAGGFAGPAPGPNVARGAPYTLNPAPNYGHCTD
                     AGDGVQLTDGEHSVGYFWVQTGTVGWSGAAQVDITIDLGRAQPIVGCSYSTAAGVAGV
                     GWPELMLILVSDDGTRWRQVGDLALLSAKHGLPPDAGYANWRFGTGDLRAYGRYFRLL
                     IGTPSPYTFCDEIEVYGGDFGEDDADPGTPVADPGQLVPDLRVHASVRRRLLSDIARL
                     RAEAESAPTATAAWAASEADRLEALATGDLGRFGSDWRAIHPLTPLHAQVYSARARVW
                     RERGEEPVRIWTSSPWDPLEPLSIPGPGPQMPSIDIGMVKGNRSHESAIGPTPIATPG
                     "
     gene            complement(1650..>2130)
                     /locus_tag="PLQ54_14930"
     CDS             complement(1650..>2130)
                     /locus_tag="PLQ54_14930"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS94613.1"
                     /translation="LAEANAYAEAHGLIPMAVSSPHVCLATAIEPFWPKCTQASHSDL
                     LWYAEHTIPVLAWSSQGRGFFLDYSGPDDRGHPDLVRVYHNESNFEKLRRVRELARAR
                     GVAPVEIALAYVLNLPAPIVALVGPATVAEIDSCVAASELEVTPDDMAWLDLRCEKA"
BASE COUNT          348 a          774 c          692 g          316 t
ORIGIN      
        1 ccaggtgccc gacgggggcc agttcctctg gatcgagctg ccgacccgca acgccaccgg
       61 ctacacccag gtcgccgggc ggcatgggct gcggctctac ccgggcgtgg cgatgggcgt
      121 cggaccgatc gccgactcgt ggctgcggtt gcccttcacc atgccgatgt cgattgacgg
      181 catctgcggt cccggtcccg ggatcgagag cggctccagc ggatcccagg ggctgctggt
      241 ccagatccgc acaggctcct cgcctcgctc gcgccagacc cgggcccgag ccgagtagac
      301 ctgggcgtgg agcggcgtca gcggatggat ggcgcgccaa tccgagccga accgccccag
      361 gtcacccgtc gccagggcct caagacggtc ggcttcactc gcggcccagg cggcggtcgc
      421 tgttggagcg ctttcggcct cggcccgtag gcgggcgatg tcgctgagca ggcgcctacg
      481 gacgctggcg tgaacgcgca gatcggggac aagctgcccg gggtcggcca ccggtgtccc
      541 cggatcggcg tcatcctcgc cgaagtctcc cccgtagacc tcgatctcat cgcagaaggt
      601 gtatgggctc ggggtgccga tcagcagcct gaagtaccgg ccgtaggctc tcaggtcgcc
      661 tgtcccgaac cgccagttgg catagcccgc atccggcggc agtccgtgct tcgccgacag
      721 cagtgccagg tcgcccacct ggcgccagcg tgtgccgtcg tcgctgacca ggatcagcat
      781 cagttccggc cagccaactc ccgcaacccc ggcagccgtg ctgtacgaac acccaacgat
      841 gggctgcgct cggcccaggt cgatggtgat gtcgacctgt gcggcaccgg accaaccgac
      901 ggtgcccgtc tggacccaga agtagccaac actgtgctcg ccgtcggtga gctgcacacc
      961 gtcgcccgcg tcggtgcagt ggccgtagtt cggcgcggga ttcagcgtgt agggcgcccc
     1021 ccgcgcaacg ttcgggcccg gcgctggtcc cgcgaagccc ccagcgactc ggccgccctc
     1081 cggccggtac tcggcggcgg ggacgatctg aacatcgtca aaccacgacg tgccttcggc
     1141 cggtcctccg tctccggcgt ggggcagtag cgacacggtc agctcgctca gcgggccgga
     1201 gttgaaccgc acactcgccc cctgccaacc accggggccc gccgagagcg ctcgggcgag
     1261 ctcaactgcc tcgcccgact ccagcacgcc ggacacggtc acagccggcc tgagcgcgtc
     1321 ggctttgaac catgcactca gtacataatc agtctgtgct tcacacatca cgacttgcgc
     1381 cacaggcgcc accacggcgc cggcggccag ccggacacgc aggctgcgct tgccgctgtg
     1441 gccatcatcg tcgaccgccg ccgcgtaacg ctccgtgcca gtcggcacgg tccactgatg
     1501 cggaaggttc cccagcgcga tgtccgagaa cgtcccgttc cgcaccaggt cggctgcccc
     1561 ggcgccaccc gccgccgcgc cgatcagcac caaagccgac aggaccacaa cgacccgcgc
     1621 catgggtttc cactccccac cagatgacgc tacgccttct cgcaccgcag atcgagccac
     1681 gccatatcgt cgggcgtgac ctccagctcg gatgcggcca cacacgagtc gatctcggcc
     1741 accgtggccg gaccgaccaa ggcgacgatg ggtgccggca ggttcagcac atatgccaga
     1801 gcgatctcga cgggcgccac gccgcgcgct cgggccagct cacggacccg gcggagcttc
     1861 tcaaagttgc tctcgttgtg gtagacgcgt acgaggtccg gatggccgcg gtcatccgga
     1921 cctgagtaat cgaggaagaa cccgcgaccc tgtgaagacc acgccaacac tggaatggta
     1981 tgctcagcat accagagaag gtcgctgtgt gaagcttgcg tgcacttggg ccagaagggc
     2041 tcgatagccg tcgccaagca cacgtgcggg ctcgagaccg ccatggggat aagcccgtgc
     2101 gcctccgcat aggcgttggc ctcggccagg
//
LOCUS       DAOSPL010000894         2128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_51970_length_2128_cov_3.808973, whole genome shotgun sequence.
ACCESSION   DAOSPL010000894 DAOSPL010000000
VERSION     DAOSPL010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51970_length_2128_cov_3.808973"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="PLQ54_14935"
     CDS             <1..977
                     /locus_tag="PLQ54_14935"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOS94614.1"
                     /translation="GRHYLTVAGPHMRPAGTFRQLHLRALPRPVETPCYQFALLSDTH
                     LSSGRHVWMNTKLDGPAKEELAATLATLKREGIAFAVIAGDMTDSGTRAQFETLGAVC
                     RGSGLPVYGCIGNHDAYLATSRPDALSLCPELFPGGKTDYVIHKPPLRLIVLDGSHWK
                     SKTGSFVDHYDASDSAGIGIKPEQIRWLKQELARDLKTPTLILWHYPMLYRGGLSSCG
                     YKLPAPATDGEVLKALRQAPNVAAVLCGHAHWNEYNQKDGIAHLVNPAFTEWPNAYRV
                     FRVYPWRMEWELRQVPNRGFVRESFVPAKAISWMISTGPEDLTGTVPF"
     gene            1360..2037
                     /locus_tag="PLQ54_14940"
     CDS             1360..2037
                     /locus_tag="PLQ54_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94615.1"
                     /translation="MVLIEVWSGRQRPSQWFRDMQENYVLQWAGMGICPSRSTWYNFR
                     DRQGPFLDQWLRQTLQIAREHGLTPARRGSLDGSLLAANASRHHLLNEERLSKRQAAL
                     ATRCARDGQGEAAEATPAWMAKTPATRVAQAHRYERAQARLQEFQAINQRQTPARRRH
                     LPSHVATGTGGDATAVGSHGDRRKLRDGVQPRDLRGREGDAVRSLAGERLHRAAKRPR
                     PANDSQS"
BASE COUNT          393 a          693 c          675 g          367 t
ORIGIN      
        1 ccggacgcca ctacctgacg gtcgccggcc ctcacatgcg gccggccggc accttccggc
       61 agttgcatct ccgggcactg ccccgccccg tcgagacgcc atgctaccag ttcgccctgt
      121 tgtccgacac gcacctttcg tcgggacgtc acgtctggat gaacaccaag ctcgacggac
      181 ccgccaagga ggaactagcc gcgaccctgg ctaccctgaa gcgcgaaggg atcgccttcg
      241 ccgtgatcgc gggcgacatg accgacagtg ggacgcgcgc gcagttcgag accctcggcg
      301 ccgtatgccg cggcagcggc ttgccggtgt acggctgcat cgggaaccac gacgcgtatc
      361 tggcgacttc gcggcccgat gccctgtcgc tgtgccccga acttttcccc ggaggaaaga
      421 ccgactacgt gatccacaaa ccgcccctgc ggctcattgt gctcgacggc tcgcattgga
      481 aatcgaagac cggcagcttc gtggaccact acgacgcctc ggactcggcc ggaatcggca
      541 tcaagccgga gcagatccga tggctgaagc aggaactcgc ccgcgacctg aagacgccca
      601 ccctgatcct ctggcattac ccgatgctct accggggcgg cctctcgtcc tgcggctaca
      661 agctgccggc cccggccacg gacggcgagg tgctgaaggc cctgcgccag gccccgaacg
      721 tggcggccgt gctctgtggg cacgcccatt ggaacgagta caaccagaaa gacgggattg
      781 cccacctcgt gaacccggcg ttcacggagt ggcccaatgc ctaccgcgtc ttccgcgtgt
      841 acccgtggcg catggagtgg gaactgcgcc aggtgccaaa ccggggtttc gtgcgtgagt
      901 cgtttgttcc cgccaaagcg atctcctgga tgatctcgac cggccccgag gacctgaccg
      961 ggacggtccc cttctgagcg gccctccgcg aagtgtattc tatcggcccc ctctcccttt
     1021 gggagagggt ttagggtgag ggccgttcgg cggcgagaca ggcgggtcga gtggctcact
     1081 cacccccggc ctggactgcc aagaacggtg atgcgatgac ctggattgtg tatcctcgat
     1141 ttgttccgcc ttcagccgtt ttctctagcc caggcgtaaa gaggctgtcc aactgctagc
     1201 atagccagga ggaggggccc gaagtaacag attcatcgac acagaggcct cctcggaatc
     1261 gagggattgg tgcagagtag atagtggaac gctctgcacc ctcgatccaa gtattcgccg
     1321 ggaggaaccg acgcggtgcg tccggatctt ttgctcggcg tggtgttgat cgaggtctgg
     1381 tcggggcggc agcgtcccag tcagtggttt cgcgacatgc aagagaacta tgtcttgcag
     1441 tgggccggca tgggcatttg cccctcgcgt tcgacgtggt acaacttccg cgaccgacag
     1501 ggcccgttcc tcgaccagtg gcttcgtcag acgctccaga tcgctcggga gcacgggctc
     1561 acaccggctc ggcggggttc gctggatggt tcgctgttgg ccgccaatgc ctcgcgacat
     1621 catttgctca acgaggagcg gttgagcaag cggcaggcgg cgttggccac acgttgtgcc
     1681 cgcgacggcc aaggcgaagc cgcggaggcg acgccggcgt ggatggccaa aacccctgcg
     1741 acccgcgtcg cacaggccca tcgctacgag cgggcccaag cgcggttgca ggagtttcag
     1801 gcgatcaacc agcggcaaac tcctgcccga cggcggcacc ttccatccca tgttgcgacg
     1861 ggcacgggag gcgatgcgac tgccgttggc agccatggtg accgacgcaa gctacgtgac
     1921 ggcgtgcaac ctcgcgatct gcgcggcaga gaaggtgacg ctgtacggtc cctggcagga
     1981 gaacgactac accgagcagc caaaagaccg cggcccgcga atgattcgca aagttgatcg
     2041 tcgagcgcga gttgagaact cgtggcatcc aaaaccagat cccccaacat gcatgccatg
     2101 acacaagcta gcagttggac agcctctt
//
LOCUS       DAOSPL010000895         2125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_52072_length_2125_cov_3.091787, whole genome shotgun sequence.
ACCESSION   DAOSPL010000895 DAOSPL010000000
VERSION     DAOSPL010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52072_length_2125_cov_3.091787"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PLQ54_14945"
     CDS             <1..700
                     /locus_tag="PLQ54_14945"
                     /inference="COORDINATES: protein motif:HMM:NF012452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HOS94616.1"
                     /translation="DPRVGRLEPAGAAHGTTVLALKYRDGVVNVGDRRATAENLVLYD
                     RADKILLYDHTLIAISGSYARAMDAVRFLRHSFNYYRRSQLQEMSLEGKLNEVTRFLV
                     GTVPDLLQGQGYVVPLISAFDVSADAGRVFFFDVAGARFETAEFGAAGSGSHRIRGAF
                     DYIVKTKGPFHEMALHEVLREALVLLDIAAELDAATGGLQKVLPSVKTVTREGIREME
                     EAELAEVVGQLGAS"
     gene            700..1485
                     /locus_tag="PLQ54_14950"
     CDS             700..1485
                     /locus_tag="PLQ54_14950"
                     /inference="COORDINATES: protein motif:HMM:NF012452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94617.1"
                     /translation="MAFTPYDWNQSIQHREDYVADRLREGSPVVGLRYAGGVALVTLR
                     RSQRKVFEIYDRLMFAGLGSQSDIEAVRVAAIDFAHQEGFRRSPDDVTIQRVVGFALS
                     PALKRQFADPFHVPAIIRGLFAEVGQTPEDDRLYLLDHDGEFEVYRHYACAAATVEQQ
                     ERMAAYAGRSFDASAGWESAVALGLRTWAVGRLPAVESEDDGCAGGATGAAVPVLPDE
                     ARITQALAEGLRDLQPEVGLLERDAPRESKFRLVPAEEIPRPG"
     gene            1495..>2125
                     /locus_tag="PLQ54_14955"
     CDS             1495..>2125
                     /locus_tag="PLQ54_14955"
                     /inference="COORDINATES: protein motif:HMM:NF015119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HOS94618.1"
                     /translation="MRERLMGLEVEYGCLVRDASLGRPEQVVELLKDYAFNDLQIGLV
                     DRHARDFAFEPAQAGGFLTNGGRLYIDAVGDHLEYATPEVTRLDDLVAHDRAGQRTLL
                     RLVDGALSRDAVSFHNNSIDHFGGHTFGCHENYAVSIPSDSLRVALTSVVSFLVSRLI
                     YAGAGRVGGHRLTRGSPRDLARQGHRVLDTLWVGDVYGVEADPGVCYQLS"
BASE COUNT          317 a          632 c          790 g          386 t
ORIGIN      
        1 cgacccccgt gtggggcgtc tcgaacccgc gggggcggcg catggtacca cggtgctggc
       61 gctcaagtat cgagatggcg tggtgaacgt gggtgaccgc cgggcgacgg cggagaacct
      121 ggtgctgtac gaccgggcgg acaagatcct gctgtacgac catacgctga tcgccatcag
      181 cgggtcgtac gcgcgcgcca tggatgcggt tcgcttcctc cggcactcct tcaactacta
      241 ccgccggagc cagcttcagg agatgagtct cgaggggaag ctcaacgaag tgacgcgatt
      301 cctggtcggc acggtacccg acctcctcca gggtcaaggt tatgtggtgc cgctgatcag
      361 cgcttttgat gtgagtgcgg acgcgggacg ggttttcttc ttcgatgtgg ccggtgcgcg
      421 gttcgagacg gcggagtttg gcgccgcggg gtcggggtcg caccgcattc gcggcgcctt
      481 cgattacatc gtgaagacca agggcccgtt tcacgagatg gcgctgcacg aggtattgcg
      541 tgaggcgctg gtgttgttgg acatcgctgc cgagcttgat gcggccaccg gtggtctgca
      601 gaaggtgttg ccgagcgtga agacggtgac ccgcgagggc atccgggaga tggaggaggc
      661 cgagctggcc gaggtcgttg ggcagcttgg ggcgagctga tggccttcac accgtatgac
      721 tggaaccaga gcatccagca ccgcgaggac tatgtggccg accggctgcg cgaggggagc
      781 ccggtggtgg gtctgcgcta cgctggaggc gtggcgcttg tcaccctccg gcgaagccag
      841 cggaaggtgt tcgagatcta cgatcggctc atgttcgccg gcctcggcag ccagtcggat
      901 atcgaggcgg tgcgggttgc cgccatcgat ttcgctcacc aggaggggtt tcggcgaagc
      961 cccgatgacg tgaccatcca gcgggtggtt ggcttcgcgt tgagcccggc gctcaagcgt
     1021 cagtttgcgg atcccttcca tgttccggcg atcatccgtg gcctgttcgc cgaggtgggt
     1081 cagacgccgg aggacgaccg tctgtacctg ctggaccacg acggcgagtt cgaggtctac
     1141 cggcactacg cgtgcgccgc ggccaccgtc gagcagcagg aacgcatggc ggcgtatgct
     1201 gggcggagct tcgatgcttc ggcgggctgg gagtcggcgg tggcgctggg gctgcggacc
     1261 tgggcggtgg gcaggcttcc cgcggtcgag tccgaggacg acggttgcgc cggcggtgcc
     1321 actggagcgg cggtgccggt gttgcccgac gaggcacgga tcactcaggc gctggccgag
     1381 gggctgcggg acctgcagcc ggaggtgggc ctactcgagc gcgacgcgcc ccgggagagc
     1441 aagttccgcc tggttcccgc ggaggagatt ccacgtccgg gataggggtg ggccatgcgc
     1501 gagcggctga tggggctcga ggtcgagtat gggtgcttgg tccgggatgc gtcgctcggt
     1561 cgtccggagc aggtcgtgga gctactgaag gactacgcgt tcaacgatct gcagatcggc
     1621 ctggtcgacc ggcacgcgcg ggacttcgcc ttcgagcccg cccaggccgg ggggttcctc
     1681 accaatggag gccgtctcta catcgacgcc gtgggcgacc atctggagta cgccacaccc
     1741 gaggtcacac ggctcgatga cctggtggcg cacgaccggg cgggtcagcg gaccctgctc
     1801 cgcctggttg acggcgcgct ctcgcgggac gcggtgagct ttcacaacaa cagcattgac
     1861 cattttggtg gtcacacctt cgggtgccat gagaactacg cggtgagcat cccgtcggac
     1921 agcttgcgcg tggcgctcac cagcgtggtc tcattcctgg tcagccgcct gatctacgcc
     1981 ggagccgggc gggtgggagg tcatcgtttg acccgcggct cgccgcgcga tctcgctcgc
     2041 cagggccaca gggtcctgga caccctctgg gtgggagatg tgtatggcgt cgaggccgac
     2101 cctggcgtct gctaccagct ttccc
//
LOCUS       DAOSPL010000896         2124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_52115_length_2124_cov_2.759304, whole genome shotgun sequence.
ACCESSION   DAOSPL010000896 DAOSPL010000000
VERSION     DAOSPL010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52115_length_2124_cov_2.759304"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1898)
                     /locus_tag="PLQ54_14960"
     CDS             complement(<1..1898)
                     /locus_tag="PLQ54_14960"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOS94619.1"
                     /translation="MRQTMGIATALLPLLLSAAASDTLRLECEDFAGPWRVQANIEGY
                     SARGFVVSNAPGIATTSMAMSVDVTAAGRYFIWARGYEGSGADRRWRVRVGGTLLAIT
                     HGDRAARGFSWQRCGDVELQPGTVEVLILDAGESYEVADAVMLTTDPNLDPAEEDRRW
                     LVLDPDEARRMVFNEIMARTRAHAAAVPVCGTLGEWHARAAEIRPRILSALGLDPLPE
                     RTPLNVNLLGETQLDGYRIQRLTFESRPGFVVTANVYVPDGPGPFPLVLCPVGHWRHG
                     KDEQTPAARSHGLAKLGYVTITYDPFGQEERAVPGNEHSEHWRLSLTGHSNMSLMVWD
                     TVRALDYMLTRPDVDPDRIACTGASGGGLNTLYYSIVDDRLDVAVPVVYITQWADFLG
                     TGAQHCPCSHVPGLGAFTDMGEMTALFAPRPQMYINALADPQFLTAGAQHAEAQARVV
                     YDLFSAGDRLRLRCFPGDHDYGKAMRETVYGFLEQHLRGQGDGTPVTESSFTPLEPDS
                     PVIRCFESGHVPETATTARQLALTWAQEAAARLPGPDALKPSALRTALKTALNPPAPF
                     QPSFEVAGQAEHHGLRVTMLRLTTQQGITLPAYLVPSGRIGPGIVIVGTSDTPMAALD
                     LAVAAHTLG"
BASE COUNT          328 a          681 c          800 g          315 t
ORIGIN      
        1 ccaagcgtgt gcgcggcgac ggccaggtcg agggctgcca tcggcgtgtc gcttgtgccg
       61 acgatcacga tgcccgggcc gatccggccc gagggcacga ggtaggccgg gagggtgatt
      121 ccctgctgag tcgtcaggcg cagcatggtg accctcaggc catgatgctc ggcctggccg
      181 gccacctcga acgatggctg gaagggcgcc ggcgggttca gcgccgtctt cagcgcggtg
      241 cggagcgcac tgggtttgag cgcgtcgggg ccggggagcc gagcagcggc ctcctgggcc
      301 cacgtcaggg caagttggcg ggctgtagtg gctgtctcgg gcacgtggcc actctcgaag
      361 caccggatga cggggctgtc cggctcgagt ggcgtaaagg aggactcggt gaccggggtg
      421 ccgtcgccct gcccacgcag atgctgctcg aggaagccgt acaccgtctc gcgcatggct
      481 ttgccgtagt cgtggtcgcc cgggaagcag cggagccgca agcggtcgcc agcactgaag
      541 aggtcgtaga ccacccgggc ctgggcctcg gcgtgttggg ctccggcggt caggaactgc
      601 ggatcggcca gcgcattgat gtacatctgc ggccgggggg cgaagagggc ggtcatctcc
      661 cccatgtcgg tgaacgcgcc gaggccgggc acgtgggagc aggggcagtg ctgagcgcca
      721 gtgccgagga agtcggccca ctgggtgatg tagacaacgg gcacggccac gtcgagacgg
      781 tcatccacga ttgagtagta cagcgtgttg agcccgccgc cggaggcacc ggtgcacgcg
      841 atgcggtcgg ggtcgacatc gggccgggtg agcatgtagt cgagtgcgcg aacggtgtcc
      901 cagaccatca ggctcatgtt cgagtgaccg gtgagtgaga ggcgccagtg ctcggagtgc
      961 tcgttgccgg gcaccgcgcg ttcctcctgg ccgaacgggt cgtacgtgat ggtgacgtag
     1021 ccgagcttgg cgaggccatg gctccgggca gctggagtct gctcgtcttt gccgtgtcgc
     1081 cagtgcccca cggggcagag gaccagggga aaaggccccg ggccatcggg tacgtagacg
     1141 ttggcggtga cgacgaaacc cggacggctc tcgaaggtga gtcgctggat tcggtacccg
     1201 tcgagctgcg tctcgccgag caggttcacg ttcagcggcg ttcgctcggg gagcggatcg
     1261 agcccgagcg cgctgaggat acgggggcga atctcagcag cgcgggcgtg ccactcaccg
     1321 agggtaccgc ataccggcac ggcggccgcg tgggcacggg tccgcgccat gatctcgttg
     1381 aacaccatcc gcctggcttc gtcgggatca aggaccagcc agcggcggtc ttcctcggcc
     1441 ggatccaggt ttgggtctgt ggtcagcatc acggcgtcgg ccacctcata ggactcgccc
     1501 gcatccagaa tcagaacctc gacggtgccg ggctgcagct ccacatcacc gcatcgctgc
     1561 cacgagaagc ctcgtgcagc gcggtcgccg tgggtgatcg ccagcagcgt accgcccacg
     1621 cggacgcgcc agcggcggtc ggcccccgat ccctcgtagc cacgggccca gatgaagtag
     1681 cggccggccg cggtcacatc gaccgacatg gccatggacg tcgtggcaat gcctggggca
     1741 ttcgagacca caaaccctcg tgccgagtag ccctcgatgt ttgcctggac gcgccacggg
     1801 ccggcgaagt cctcgcactc cagccggagt gtgtcggacg ccgcggcgga caggagcagc
     1861 ggcagcagcg cggtggcgat gcccatggtc tggcgcacgg agccctcctg cgcgagacgc
     1921 gagacgagcg ggagtccgtg actggttcga gcgggagcgg gcgggacctg cccgcgccgc
     1981 gggccggccc tagcgagggc gccatcgctg ccgggtcgcc gccggcttcg ccggcccggt
     2041 gctggagccc gccgaggtaa cgcccggccc tcgggaatcg ggccgctcgg gcgctccccc
     2101 gaccacacgg atcgggaagg cgat
//
LOCUS       DAOSPL010000897         2123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_52156_length_2123_cov_2.407157, whole genome shotgun sequence.
ACCESSION   DAOSPL010000897 DAOSPL010000000
VERSION     DAOSPL010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52156_length_2123_cov_2.407157"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PLQ54_14965"
     CDS             <1..745
                     /locus_tag="PLQ54_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94620.1"
                     /translation="TSLFGQVASFSVGTHDWEFRSVTVQPEKPIRSLTYNILFRYRTG
                     TVWFDELSLKEGADPDRAAERLQNGGLEDTVRASKLIDVAATANTLEVPAYSGRVYLY
                     ASGSEDQLQTVGPRLTVTTSPALGEVRFRVDGFDYWTCSGRWTTEYVLGPDFGRFSIL
                     FDGPGTHTVEVVDVVPSPLRTPAGYGAGERLGPFMDPSQPTRPAEGRSFRFRGWTGAL
                     TTAEARVEVDVSSDLTLTAEFDTEEAPGQ"
     gene            complement(804..>2123)
                     /locus_tag="PLQ54_14970"
     CDS             complement(804..>2123)
                     /locus_tag="PLQ54_14970"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HOS94621.1"
                     /translation="LNDALEKGFLRSRFADRMQFCIDWCNEGHCTEFRPLDFSDVSLE
                     GFIRYVAERYFARPNYLRVGGKPVVLIHEAWRLANAHGGWAGCAEALDRMRGIAREYG
                     HPGVYFVAVQNHPILASFGEGGFDAVTAYAYGFCDVPWGGPDRSLPFEALYSRHDDAM
                     AEARRRAHDQGLAYIPTAWVGWDDAARTRERAVRTTGNTPSAFRRMLERLPEYVDDEP
                     RLALFESWNEWGEGGAAEPGTEYGFGRLDAIRDVLTDARGPHVDPVPSPEDRDAIQTS
                     LSFDDVNEHYWRRYARRLGLSGGLRLGFDSVHDLWLRPGSQLAHVTVEGGRLRAQSIG
                     ADPSFLGPPCMGLPADRVRRVAVTMGATAGTEAQLFWRTHMHEDFTEAQSVRFPIIAD
                     GLMHEYRIDVSASPLWTGRVEQLRLDPTNAAAEIAVDAFVAGVEGEL"
BASE COUNT          336 a          754 c          707 g          326 t
ORIGIN      
        1 gacgagcctg ttcggccagg tagcctcctt ctcggtgggc actcatgatt gggagttccg
       61 ctcggtgacc gtgcagcccg agaagccgat ccggagcctc acctacaaca tcctcttccg
      121 ctaccgcacg ggcaccgtct ggttcgacga gctgtcactc aaggagggcg ccgatcccga
      181 tcgcgccgcc gagcggctgc agaacggcgg cctcgaggac actgtccgcg cctccaagct
      241 catcgacgtc gctgccacgg ccaacacgct cgaggtcccg gcctactccg gccgcgtgta
      301 cctttacgcc tcgggctccg aggaccagct ccagaccgtc ggccctcggc tcacggtcac
      361 cacatccccg gccttgggag aggtccgctt ccgcgtggac gggttcgact actggacctg
      421 ctccggccgc tggaccacag agtacgtgct cggtccggac ttcgggcggt tcagcatcct
      481 attcgatggc ccgggaaccc acacggtcga ggtcgttgat gtcgtgccct cgccactccg
      541 gacgcctgcc gggtacggtg cgggtgagcg gctggggccc ttcatggatc cctcgcagcc
      601 cacccgtccg gcggaggggc gctccttccg cttccgtggc tggaccgggg ccctgaccac
      661 cgcggaggca agggtcgaag tcgatgtgtc gtcggatctg accctgacgg cggagttcga
      721 caccgaggaa gcaccagggc agtgagccgg ggcaacccgc cggcagcccg tgttccggtc
      781 gtgcccgatg gcagcgctcc ggcctacaac tcgccttcca cgcccgccac gaacgcatcg
      841 acggcgatct cggccgccgc gttcgttggg tcgagccgga gctgctccac ccggcccgtc
      901 cacaagggtg atgctgacac gtcgatccgg tactcgtgca tgagcccgtc agcgatgatg
      961 gggaagcgca cgctctgggc ctcggtgaag tcttcgtgca tgtgggttcg ccagaagagc
     1021 tgggcctcag ttccagccgt cgcgcccatg gtcacggcta cccgccgcac gcggtcggcc
     1081 ggcagcccca tgcacggcgg accgaggaac gacggatcag cgccgatcga ctgcgcacgg
     1141 agacgcccgc cctccacggt cacatgggca agctgcgagc ccggccggag ccacaggtcg
     1201 tgcaccgagt cgaacccaag ccgtaggcct ccgctgagcc ccaaccgccg cgcgtaccgg
     1261 cgccagtagt gctcgttgac atcgtcgaac gagaggctgg tctgtatcgc atcgcggtcc
     1321 tcgggcgatg gcacgggatc cacatgtggc ccgcgggcgt cggtgagcac atcgcggatg
     1381 gcatcgagcc ggccgaaccc atactcggta ccgggctcgg ccgctccgcc ctcgccccac
     1441 tcgttccacg actcgaacag ggccaggcgc ggctcgtcgt cgacgtactc ggggagccgc
     1501 tcgagcatgc gccggaacgc cgaaggcgtg ttgccggtgg tccgtaccgc ccgttcgcgg
     1561 gtccgcgcgg cgtcatccca gccgacccag gccgtgggga tgtaggccag gccctggtcg
     1621 tgcgcacgcc gccgtgcttc cgccatggca tcatcgtgcc gggagtacag cgcctcgaag
     1681 ggcagggagc ggtccggccc gccccacggc acgtcgcaga agccgtaggc gtaggcggtg
     1741 acggcatcga atccgccctc gccgaaggac gccaggatgg ggtggttctg cacggcgacg
     1801 aagtagactc ccgggtggcc gtactcgcgc gcgatgccac gcatccggtc cagcgcctcg
     1861 gcgcagcccg cccaaccacc gtgggcatta gcgagccgcc aggcctcgtg gataagcacc
     1921 acgggcttgc cgcccacccg caggtagttg ggccgggcga agtaacgctc agccacgtag
     1981 cggatgaagc cttcgagcga gacatccgag aagtccagcg gacgaaactc ggtgcagtgc
     2041 ccctcgttgc accagtcgat gcagaattgc atgcggtcgg cgaatcggct gcgcaggaag
     2101 cccttttcga gcgcatcgtt cag
//
LOCUS       DAOSPL010000898         2117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_52335_length_2117_cov_3.124636, whole genome shotgun sequence.
ACCESSION   DAOSPL010000898 DAOSPL010000000
VERSION     DAOSPL010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52335_length_2117_cov_3.124636"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2117
                     /locus_tag="PLQ54_14975"
     CDS             <1..>2117
                     /locus_tag="PLQ54_14975"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOS94622.1"
                     /translation="VRVGDANRLDMEMVVDTVSERLAYASGYAFHNLGGIDRSVRLIA
                     LPQTHLRSLTVEQTLDDALTMATIRVRSSVARSGSTPAANLRLELRLRDERGRPVAVE
                     GGISEVEPARPGARAEASAQIAHPLLWSAEKPHLYRLTVRLLDGDTLLEEVERSVGFR
                     RIEIRGAQLFVNSQRVKLAGACHHEIDPLTGRAATARHAEEDVRLAKGANLDYLRTSH
                     YPPTEELLDAADRLGVYLEVEAPFCWVSNDTDPEHLRAVLTPTSAMVDAAHSHPSVLL
                     WSIANESQYNPLFEISTAMVKQLDPSRPTTFNNPDPKQIADIANLHYAAVPYEQHLQD
                     DPRPMVLGEYFFPICHEQTDVAVDPGLRELWGAGHRDPSSAWGKDCASSFGGAFMQPG
                     LPPGTWTEMVRSDRVIGGAIWAFLDEPFYLPGGKKVGYAWHHGFWGLVDAWRRPKPEW
                     WLAKQIFSPVWFVDSMAPFSGDEESVRVRVENRYAFTDFAELEFASRTPAGEEPFDLS
                     LAPGASAAVAVPVPRGSRRGQVLQIEARGRGGDLVTIAAIVLGEREHHEAPAPDAGCP
                     RLTDEGSAFRIEGDGFVFVVDKTRGKLADGSVSALVSLPTLHLTRFDFGDLAGPNAAP
                     YEVLPQRETRQVERVTAETVSEGVMLTIRERFEGFSGETRWLIDRAGVGRVAYDYIHS
                     GPELAAREIGVRKALGARRRHIL"
BASE COUNT          322 a          696 c          765 g          334 t
ORIGIN      
        1 cgtacgcgtg ggcgatgcga accgtctgga catggagatg gtcgtcgaca cggtgtcgga
       61 gcgcctcgcc tatgcctcag gctatgcctt ccacaacctg ggcgggatcg accggtcggt
      121 gcggctcatc gcgctgcccc agacccacct gcgcagtctg acggtcgagc agacgctcga
      181 tgatgccctg accatggcca ccattcgcgt gcgctcgtcc gttgcccgtt cggggagcac
      241 gcccgcagcg aacctgaggt tggagctacg gctgcgggac gaacgcgggc ggccggtggc
      301 agtggagggc gggatatccg aggtggaacc ggcccgcccc ggcgcccggg ccgaggcgtc
      361 ggcccagatc gcgcatccgc tgctgtggag cgcggagaag ccgcatctgt accgactgac
      421 ggtccggctg ctggacggag acacgctgct cgaggaggtt gagcggtcag tcggcttccg
      481 gcggatcgag atccgcggcg cgcagctctt cgtgaacagc cagagggtga agctggcggg
      541 tgcgtgccat catgagattg acccgctgac gggccgggcg gccacggcgc ggcatgcgga
      601 ggaggacgtg cgtctggcca agggcgcgaa cctcgactac ttgcgcacct cgcactaccc
      661 gcccacggag gagctcctcg acgccgccga ccggctcggc gtgtacctgg aggtcgaggc
      721 gcccttctgt tgggtgagca acgacaccga tccggagcat ctccgggcgg tgctcacgcc
      781 cacgtcggcc atggtcgacg ccgcacattc gcacccgtcg gtgctgctgt ggtccatcgc
      841 caacgagtcg cagtacaacc cgctgttcga gatctcgacg gccatggtga agcagctcga
      901 cccatcgcgg ccgacgacgt tcaacaaccc cgacccgaag cagatcgccg acatcgccaa
      961 tcttcactat gcggcggttc cgtatgagca gcacctgcag gacgacccgc ggccgatggt
     1021 tctcggcgag tacttcttcc cgatctgcca cgagcagacc gatgtggccg tcgatccagg
     1081 ccttcgggag ctgtggggtg cggggcaccg ggatccgtca tcggcctggg gcaaggactg
     1141 cgcgtccagc ttcggcggcg ccttcatgca gccgggcctt ccgccgggca catggaccga
     1201 aatggtgcgg tcggaccgcg tgatcggcgg cgccatctgg gcgttcctcg atgagccatt
     1261 ctacctgccg ggcgggaaga aggttgggta cgcctggcac cacgggttct ggggcctggt
     1321 cgacgcctgg cgccggccca agcccgagtg gtggctggcg aagcagatct tcagcccggt
     1381 atggttcgtg gactccatgg caccgttcag tggcgacgag gagagtgtca gggtgcgagt
     1441 cgagaaccgg tatgcgttca cggacttcgc cgaactcgag ttcgcgagcc ggacgccagc
     1501 cggcgaggag ccgttcgacc tctctctggc gcccggcgcg tcggcagccg tggccgtccc
     1561 cgtgccgcgg ggctcgcgcc ggggccaagt gctgcagatc gaggcgcgcg gtcgcggggg
     1621 cgacctggtc accatcgcgg ccatcgtcct cggtgagcgc gagcaccacg aggcgcctgc
     1681 tccggacgcc ggatgccccc gactcactga cgaggggagc gcatttcgca tcgagggcga
     1741 tggcttcgtg ttcgtcgtgg acaagaccag ggggaagctc gcagacggga gcgtctcggc
     1801 cctggtttcg ttgccgacgc tgcacctgac gcggtttgac ttcggcgacc tggccggccc
     1861 caacgcggcc ccctacgagg tgctgcccca gcgggagacg cggcaggtcg agcgcgtcac
     1921 ggcagagacg gtctccgagg gtgtgatgct gaccatccgc gaacggttcg agggcttctc
     1981 cggggagacc cgctggctca tcgaccgggc cggtgtgggg cgtgtggcgt acgactacat
     2041 ccactcgggc cctgaactgg cggcgcggga gatcggcgtg cggaaggcgc tgggcgcgcg
     2101 ccggcgccac atcctga
//
LOCUS       DAOSPL010000899         2115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_52414_length_2115_cov_2.747573, whole genome shotgun sequence.
ACCESSION   DAOSPL010000899 DAOSPL010000000
VERSION     DAOSPL010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52414_length_2115_cov_2.747573"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PLQ54_14980"
     CDS             complement(<1..501)
                     /locus_tag="PLQ54_14980"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HOS94623.1"
                     /translation="MRWRFSLCVAVCMLVTAMVAHPTPAAPGGMVPPKGLRMTEEVRR
                     AAQNLARLSARPRPAGQVRAPAVGQGKCLVDLVDFSDNAGTRSASDFQNMLFTDGTYA
                     TGSMADFYRENSYGLFTLTGTVSGWYRAPDTYAHYANGDYGATYVADLVRDLVDLMDP
                     DVDFSQY"
     gene            complement(674..>2115)
                     /locus_tag="PLQ54_14985"
     CDS             complement(674..>2115)
                     /locus_tag="PLQ54_14985"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS94624.1"
                     /translation="NGYQFIPAEWSLDAYAWQALVLCALAESGRFVPCSADAHARAWQ
                     AAGQPLSRLSDAELVRAWQASEAPVAVVGKILRSADGAVDGVHVSVLKSASRALRPGG
                     IDWGDGMAAGEDLEARVMDALARALGQPSIGPRLAHRVARVTPAARSALAGALAKLDG
                     ATGSPEAVVEAVNALSPLAGDLPALAAARGVVEHVCRAALAVRDDLAAAVLYLGYAHL
                     AAGDLDQALGACTRALNMEPGCRDAYNLMGLAYEGRNRLDEAEQAFDRASKDGGPAIA
                     AYNHARIRELRGDRAAAEAEYARVAQQTDDRRAAALAHLALAGPLLDAGNGQGALEHL
                     DVAIQLAPALAAAHYNRGLALQQLGRLDEAVGSYAESVRLEPGNAAAHNNLGVTYYRL
                     DRTPEAVAAYRRALELDPAFYAVRRNLAVAHEKARDWPAALECWQQYLDAAVADRQKG
                     GRVTEDDLVLARQHVEQIRALQEQAEPAG"
BASE COUNT          316 a          755 c          718 g          326 t
ORIGIN      
        1 atattgcgag aagtccacat cggggtccat caggtcaacc aggtcgcgga ccaggtcggc
       61 cacataggtg gcaccgtagt caccattagc atagtgggca taggtgtctg gagcgcggta
      121 ccatcccgac acggtgcccg tgagggtgaa caggccgtag gagttctctc tgtagaagtc
      181 cgccatgctg cccgtggcgt atgtgccatc agtgaacagc atgttctgga agtcggatgc
      241 cgaccgggtg cccgcgttgt cgctgaagtc cacgaggtcg acgaggcact tgccctggcc
      301 cacagccggc gcgcgaacct ggccggcggg ccggggtctc gcggagagac gcgccaggtt
      361 ctgggcggcc cggcggacct cctcggtcat gcgtagtccc ttgggcggca ccataccgcc
      421 cggagcagcc ggcgtggggt gtgccaccat cgcagtgacc agcatgcaca ctgcgacgca
      481 gagcgagaag cgccagcgca cggtgaccag tccttcccat cgaacgctat ccgccttgac
      541 agaggtggtc tgcttatctc atctaggtag tgcaagcgag gacggccggc agaccgtcgc
      601 tgtctgccta ggtcttcgta cctcttgggt tgcggacctt ccgcgcggct cgccgcggcg
      661 agtagcccgt gcgttacccc gcgggttccg cctgctcctg gagcgctcgt atctgctcga
      721 cgtgttgccg ggccagaacc aggtcgtctt cagtcacgcg gccgcccttc tgcctgtcgg
      781 cgacggcggc gtccaggtac tgctgccagc actcgagtgc tgcgggccaa tcgcgcgcct
      841 tctcatgcgc cacggccagg ttccggcgga cggcatagaa cgccgggtcc agctccagcg
      901 cgcgccgata ggccgcaacg gcctccggcg tgcggtccag ccggtagtag gtgactccca
      961 gattgttgtg ggccgccgcg tttccaggct cgagtcgcac ggactcggcg taggacccga
     1021 cggcctcgtc cagacgcccc agttgctgca gggctagccc ccggttgtag tgggcggccg
     1081 ccagtgccgg agcaagctgg atggcgacat cgaggtgctc gagcgctccc tgcccattgc
     1141 ccgcatcgag gagcggcccg gcgagagcca ggtgcgccag agccgcggcg cgccggtcgt
     1201 cggtctgctg ggccacgcga gcgtactcgg cctcggcggc agcccggtcg ccccgcagct
     1261 cgcggatccg ggcgtggttg taggccgcga tggccgggcc gccatccttc gaagcccggt
     1321 cgaatgcttg ctcggcctca tcgaggcgat tgcggccctc gtacgccagc cccatcaggt
     1381 tgtaggcatc gcggcagccc ggctccatgt tcagtgcccg tgtgcatgcg ccgagcgctt
     1441 ggtccaggtc gcccgccgcc aggtgggcat accccagata gagcacggcc gccgcgagat
     1501 cgtcacggac agccagcgcg gcacggcaca cgtgttcgac cacgccgcgc gccgccgcca
     1561 gggccggaag gtctccggcg agaggcgata gcgcgttcac ggcctcgacc acggcctcgg
     1621 gcgacccagt ggcgccgtcc agcttggcca gagccccggc cagggccgac cgtgcggctg
     1681 gcgtgacgcg tgccacccga tgggccaggc gtggtccgat ggacggctgc cccagcgccc
     1741 gcgccagggc gtccatcaca cgggcctcca ggtcctcgcc cgccgccatg ccgtcgcccc
     1801 agtcgatacc ccctggccgc aacgcccggc tcgccgactt cagtacggac acatggacgc
     1861 cgtccacagc accgtcggcc gaccgcagga tcttccccac cacggccacc ggggcctcgg
     1921 aagcctgcca agccctcacg agttcggcat ctgacagccg cgaaagcggc tgacccgccg
     1981 cctgccatgc gcgggcgtgc gcgtcggcgc tgcacgggac gaaccggccc gactccgcca
     2041 gtgcgcacag caccagggcc tgccaggcgt aggcgtccag gctccattcc gcggggatga
     2101 attgatagcc gttca
//
LOCUS       DAOSPL010000900         2113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_52487_length_2113_cov_3.237123, whole genome shotgun sequence.
ACCESSION   DAOSPL010000900 DAOSPL010000000
VERSION     DAOSPL010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52487_length_2113_cov_3.237123"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PLQ54_14990"
     CDS             complement(<1..381)
                     /locus_tag="PLQ54_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOS94625.1"
                     /translation="MVWIAMPAQVETCVLGWAHEYPAHVSVTTLSQFDGHTVYAVTVT
                     GPSAGGSVKPGLLCAVPHAHEPAGTAACMSVLCQLITGQTLDRPGLQSLLTAAEQGAF
                     DILIVHELSRLSRSSVYDTFSIFDH"
     gene            complement(491..1426)
                     /locus_tag="PLQ54_14995"
     CDS             complement(491..1426)
                     /locus_tag="PLQ54_14995"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOS94626.1"
                     /translation="MAFGSERLCLDPAPRPWVTRAFESPLMLSVMLLGITAVWGWTFT
                     VVKDAVAMCGPMPFLAMRFAVAALAMVALSRGRLHRPSVVAGVGVGCVLAVSYVFQTL
                     GIELTSATNSGLITGLFVVAGPVLNRLLFGVRASRIAWTAVCSSFVGLALLAGTGARP
                     PTSGDLLTLCCAMTFGLHIALLDRYAKHLEPVSMATGQIALAAAVFGIVSPMVEPVRL
                     PPPGVWAPVLLTGVVGTALGFLTQTAAQRRLPAVRAAVILAMEPLFAAVFGYCVAGDR
                     LTPPQIIGATVMISAFMVVECAPLIANGRQNGGGH"
     gene            complement(1532..>2113)
                     /locus_tag="PLQ54_15000"
     CDS             complement(1532..>2113)
                     /locus_tag="PLQ54_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94627.1"
                     /translation="EVGRAEQAALDSIFSRLTDYQQLAIEWEFGGGPSDADRQALLES
                     TRRQMMGMQRLVDQLLAPQLEAIWSIRNTQQYHRQRAAVAQQILEGLVGQRMLMGGPE
                     MADQYIGNLFQYFDHIRAMPPDQFEMGLRSGMLSQTLLRSLGVGFGGDMAQIPRVPEQ
                     ELRDLLLDPYCITMITTRADTMMRLVQDRRRGG"
BASE COUNT          394 a          668 c          745 g          306 t
ORIGIN      
        1 gtggtcgaag atggaaaagg tgtcgtacac cgacgaacgc gagagccgtg acagctcgtg
       61 gacgatcagg atgtcgaacg ccccttgctc cgcggcagtc agcagtgact gcaagccggg
      121 ccggtcgagg gtctggccgg tgatgagctg gcacagcacg ctcatgcaag ccgccgttcc
      181 ggccggttcg tgtgcatggg gcaccgcgca gagcagccca ggcttcacgc tgccgccggc
      241 agaggggccc gtgaccgtaa ccgcatagac cgtgtgcccg tcgaactggg acagagtggt
      301 gacggagacg tgggcggggt actcgtgggc ccaaccaaga acgcacgtct cgacttgggc
      361 gggcatggcg atccagacca aggccgttca cctcaggcag ccgttcgcat ggtcttgcac
      421 cggcacctcg cgcgagatga ttgccgcgcg aatgtcgtag cgggtccacg ccccattccc
      481 tgggccggtg tcagtggccg ccgccgttct ggcggccgtt ggcgatgaga ggggcgcact
      541 caacgaccat gaaggcgctg atcatcacgg tggccccgat gatctggggc ggcgtgagcc
      601 ggtcaccggc gacgcagtag ccgaacacgg cggcgaagag gggctccatg gcgaggatca
      661 cggcggcacg gacggcgggc agacgtcgct gggcggccgt ctgggtgagg aagccgaggg
      721 ccgtaccgac aacaccagtc aggagtaccg gggcccacac gccgggagga ggcaaccgaa
      781 ccggctccac catgggcgag acgatgccga acacggcggc ggccaaggcg atctgccccg
      841 tcgccatcga caccggctcc aggtgcttgg catagcgatc gagcagggcg atatgcaggc
      901 cgaaggtcat cgcgcaacac agggtgagca ggtcgcccga tgtgggaggc cgtgctccgg
      961 tgccggcaag cagagccagg ccgacgaagc tcgagcacac ggccgtccag gcaatcctac
     1021 tggcccggac gccaaacagc agacggttga gtacggggcc cgcgacgacg aagagcccgg
     1081 tgatcaggcc tgagttggtg gcggacgtga gctcgatgcc gagggtctgg aagacgtagg
     1141 acacggccag cacacatccg acaccaactc cggcgacgac cgaagggcgg tggaggcggc
     1201 cgcgggagag cgccaccatc gccagggcgg ccaccgcgaa acgcatggcg aggaatggca
     1261 tggggccgca catggccacg gcatccttga cgacggtgaa cgtccagccc cacaccgcag
     1321 tgatgcccag gagcatcacc gaaagcatga gtgggctctc gaatgcgcga gtgacccacg
     1381 ggcgtggggc tgggtcgagg cacaatcgtt cggagccaaa tgccatgtgc cgcgagacct
     1441 cctccgggat gggggcgtga aaaagaccgc ggcggaacgg ggggaccgtt ccgccgcggt
     1501 cgattggcct atctggtgtc gagctggtgg cctatcctcc tcggcgccgg tcctggacca
     1561 ggcgcatcat cgtgtcggcc ctcgtagtga tcatggtgat gcagtacggg tccagcaaca
     1621 ggtcgcgcag ctcttgctcg ggcacgcggg ggatctgagc catgtcgccc ccgaacccga
     1681 cgcccaggct acgcagcagc gtctgtgaga gcattccgga tcggaggccc atctcaaact
     1741 ggtccggcgg catcgcacga atgtggtcga agtactggaa caggttccct atgtactgat
     1801 cggccatctc gggtccgccc atcagcatgc gttgtccgac cagcccctca aggatctgct
     1861 gggcaaccgc ggcccgctgg cgatggtact gctgggtgtt ccggatgctc cagatcgcct
     1921 cgagctgcgg ggccagcagt tggtccacca gcctctgcat gcccatcatc tggcggcgcg
     1981 tactctcgag caatgcttgc cggtcggcat ccgagggccc gcctccaaac tcccactcga
     2041 tggccagctg ctggtagtcg gtgagacgcg agaagatcga gtccagggcg gcctgctcgg
     2101 cccggccgac ctc
//
LOCUS       DAOSPL010000901         2110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_52566_length_2110_cov_3.164964, whole genome shotgun sequence.
ACCESSION   DAOSPL010000901 DAOSPL010000000
VERSION     DAOSPL010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52566_length_2110_cov_3.164964"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..>2110)
                     /locus_tag="PLQ54_15005"
     CDS             complement(81..>2110)
                     /locus_tag="PLQ54_15005"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOS94628.1"
                     /translation="PVVRFALGLASLFEHVALPEWSGEALYPCSGSLYSGGQAVEWHY
                     SSCLLYDPGRLRAKQAATDDQAVRDALEAVHLALADYPRVGGYTHSIPNYRRILAEGL
                     SSYRDRVRRARAAAESRDERERIDFCRAMEILLDGVETLHRRTRARVREASPTDPQAR
                     AQQEALLAALERVPLAPATRFYEALVSVNWILYLDGPDDLGRFDQDLWPFFKRDRAAG
                     LVTDGQARDWIGLLWDNINRTHAWNVALGGRLEDGRSGITDLTRICVEAGRGRRRPNL
                     ALRLHRQAPDTIWDAALDSIASGNGLPALYNEEGYTLAIREAHLNLPESDLPDFAFGG
                     CTELMVHGKSNCGSLEGDLNLPLVLVATLHKDLARSATFDELLLAYRRDIQQCIRAQV
                     EVWNANQETKARWQPQVLRSLLIDDCIDNAREYNAGGARYNWCVVNVMGLANAIDSLA
                     AVREVVYDRGEIAPEDLLRALESDFVGYEPIRKRLETCPRFGNDDPRADDLAESLSGF
                     VFDELRMYAPWRGGRYLPSCLMFVTYAMFGKPVGATPDGRRAGAPIADSAGAMQGRDR
                     HGPTALLRSVARIKHHLAPGTLVVNIRLARSFLKTPQSRRKLRELVETYFALGGLQLQ
                     INVVDQKTLRDAYEHPENHGDLIIRVGGYSEYWQHLDDDLRRSILERIEHE"
BASE COUNT          333 a          646 c          797 g          334 t
ORIGIN      
        1 ccggtcgcgt gccggcggcc gtcctcggcc gcgacggggg cccccgacta cgccgacgtg
       61 gccgtccgcg ctccggccgt ctactcgtgc tcgatgcgct cgaggatcga acgacgcagg
      121 tcgtcgtcga ggtgctgcca gtactcggag tagccaccga cgcggatgat gaggtcaccg
      181 tggttctcgg ggtgctcgta ggcatcgcgt agggtcttct ggtcgacgac gttgatctgg
      241 agctggaggc cgcccagggc gaagtaggtc tcgaccagct cgcggagctt acggcgcgac
      301 tggggcgtct tgaggaagct gcgggccaga cggatgttga ccaccagagt ccccggcgcc
      361 aggtggtgct tgatgcgagc cacggagcga aggagtgccg tgggcccgtg gcggtcgcgg
      421 ccctgcatgg cgccggcgga gtcggcgatg ggcgcaccgg cgcggcggcc atcgggcgtg
      481 gcccccacgg gcttgccgaa catggcgtag gtgacgaaca tgaggcacga gggcaagtag
      541 cggccgccac gccagggggc gtacattcgc agttcgtcga agacgaagcc cgacaggctc
      601 tcggcgaggt catcggcgcg gggatcgtcg ttgccgaaac gggggcacgt ctcgagcctc
      661 ttgcggatgg gctcgtatcc gacgaagtcg ctctcgagcg cgcggaggag atcttccggg
      721 gcgatctcgc cgcggtcgta gacgacctcg cggacggcgg ccagggaatc gatggcattg
      781 gccaggccca tgacattgac gacacaccag ttgtagcggg caccgccggc gttgtactcg
      841 cgggcgttgt cgatgcagtc atcgatgagc agactccgca gaacctgggg ctgccagcgc
      901 gctttggtct cctggttggc gttccagacc tcgacctgag cccggatgca ctgctggatg
      961 tcgcgccggt aggcgagaag cagctcgtcg aaggtcgcag agcgcgccag gtccttgtgg
     1021 agtgttgcca cgaggacgag gggcaggttg aggtcgccct cgagcgagcc gcagttgctc
     1081 ttgccgtgga ccatgagctc cgtgcacccg ccgaaggcga agtcgggcag gtcggactcg
     1141 ggcaagttca ggtgagcctc gcggatggcg agcgtgtagc cttcttcatt gtagagcgcg
     1201 gggagtccgt tcccgctggc gatggagtcc agcgcggcgt cccagatcgt gtccggggcc
     1261 tgacggtgga gccgcagcgc cagattgggt cgccgtcgcc ctcggccggc ctcgacacag
     1321 atgcgcgtga ggtcagtgat gcccgagcga ccatcctcga gccgcccgcc gagagccaca
     1381 ttccatgcat gcgttcggtt gatgttgtcc cagaggaggc cgatccagtc acgtgcctgg
     1441 ccgtccgtca cgagcccggc ggctcggtca cgcttgaaga agggccagag gtcctggtcg
     1501 aagcgaccca ggtcgtccgg accgtcgagg tagaggatcc agttcaccga cacgagggcc
     1561 tcatagaagc gggtcgcggg cgccagcgga acgcgctcga gggcagcgag gagggcctcc
     1621 tgttgggccc gggcctgggg atcggtgggc gaggcctcgc gcacgcgcgc cctggtgcgg
     1681 cggtggagcg tctcgacgcc atcgagcagg atctccatgg cacggcagaa gtcgatgcgt
     1741 tcgcgctcgt cacgactctc ggcagcggcg cgggcacgcc ggacgcgatc tcgatagctc
     1801 gatagtccct cggcgaggat ccggcggtag ttggggatgc tatgcgtgta gccgccgacc
     1861 cgggggtaat cggcgagggc caggtggacg gcctcgaggg catcgcggac ggcttggtcg
     1921 tcggtggcgg cctgtttcgc gcgcagacgg cctgggtcgt agagcaggca cgacgagtag
     1981 tgccattcga cggcctggcc gcccgagtat agggagcccg agcaggggta cagggcctct
     2041 ccagaccact ctgggagcgc cacatgctcg aagaggctgg cgaggcccag ggcaaatcgc
     2101 accacaggag
//
LOCUS       DAOSPL010000902         2105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_52741_length_2105_cov_2.867805, whole genome shotgun sequence.
ACCESSION   DAOSPL010000902 DAOSPL010000000
VERSION     DAOSPL010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52741_length_2105_cov_2.867805"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PLQ54_15010"
     CDS             complement(<1..585)
                     /locus_tag="PLQ54_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00375 family protein"
                     /protein_id="HOS94629.1"
                     /translation="MRTYFADLHIHIGRSSDGRAVKVTASRDLTFENICIECVDRKGI
                     EIAGMIDCASPGVLRDIDLLLESGQMVPQPGGGLRYRDRLTVILGSELETREVDGGLS
                     HVLCYFPTVDAIRGFAHALSGYVSNLQLSSQQCHMPANDLALIAVDHGGFVVPAHAFT
                     PHKSLYGSCADRIGRVFGPKALSGIAAIELGLSAD"
     gene            complement(631..1374)
                     /locus_tag="PLQ54_15015"
     CDS             complement(631..1374)
                     /locus_tag="PLQ54_15015"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOS94630.1"
                     /translation="MRYAPGLLVGAVAVAALAARVPAVGRTATVRALIVTGGHDFERD
                     AFFAMFSAEEGLEWREALQPAASDEYTESRAKGYDAIVLYDMMQEITDAQKANLLARI
                     REGKGLVVLHHALGSYDTWPEWWKVVGGHYLMTPRTVDGRDLPGSTFREGVDIRVQIA
                     DRHHPVTRGLEDFTIHDEVYGGMWVSPDAHLLLRTDHPESTPEMAWAGRLGRGRVAAI
                     QLGHGPSAYANPSYRRLVGNAIRWCARKD"
     gene            complement(1379..>2105)
                     /locus_tag="PLQ54_15020"
     CDS             complement(1379..>2105)
                     /locus_tag="PLQ54_15020"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS94631.1"
                     /translation="ITGLDFFEGQQFSPSVSLESDPYVLKDLIDEHGLRCSCLSAHWP
                     IWSYQCIDYMIKAIRFARVLGCSAIATTDSEHIPDTYSLDEWMGVYKYHFDCVVPSAE
                     RHGVYVCVEPHGVLTQRPDSLLRIVTQTRSPMMGVNFDTGNTFIAGHNPPDFLRQVID
                     HVHHMHIKEVSHELAAALRGESTGIAASSAAIGEGANAEGIRQCVRMLKEHGKNVVCS
                     LEVSGDAPTERSVPWFRRVLEEA"
BASE COUNT          361 a          697 c          685 g          362 t
ORIGIN      
        1 atccgcggac aggcccagct caatagccgc gatgcccgac agggccttcg gcccaaacac
       61 acggccgatc cgatccgcgc atgagccgta gaggctcttg tgcggtgtga acgcgtgcgc
      121 aggaaccacg aagcccccat ggtccacagc aatgagcgcc aggtcgttcg cgggcatgtg
      181 gcactgctgc gagctgagct gcagattgct tacataaccc gatagcgcgt gggcgaagcc
      241 gcgaatggcg tcgaccgtgg ggaagtagca gagcacgtgg gacaggccgc cgtcgacctc
      301 acgggtctcg agctcggagc ccaggatgac ggtcaatcgg tcacgatagc gcagcccgcc
      361 gccgggctgc gggaccattt ggccgctctc gagcaagagg tcgatgtcgc gcagcacgcc
      421 cggcgaggcg cagtcgatca tgccggcgat ctcgatgcct ttgcggtcca cgcactcaat
      481 gcagatgttc tcgaatgtca gatcgcgaga cgccgtaacc ttgacggccc ggccgtccga
      541 tgagcggccg atgtggatgt gtaggtcggc gaagtacgtg cgcaagcccg gcgatgcacc
      601 tccgacgaga cggcagctct cacgacgccc tcagtctttc cgcgcgcacc accggatggc
      661 gttgcccacc agtcggcgat agctcgggtt cgcgtaggcc gaggggccgt ggccgagttg
      721 gatggcggcc acacggcctc gacccagcct gcccgcccac gccatctccg gcgtactctc
      781 cgggtggtcg gtgcgcagga gcaggtgggc atcggggctc acccacatcc caccgtagac
      841 ctcgtcatgg atggtgaagt cttcgaggcc acgagtcacc gggtgatgcc ggtcggcgat
      901 ctgcacgcgg atgtccactc cctcccggaa tgtcgatccc ggcaggtccc gcccgtccac
      961 cgtgcggggg gtcatgaggt agtgcccgcc cacaactttc caccactcgg gccatgtgtc
     1021 ataggagccc agagcgtggt gcagaacgac tagacccttg ccctctcgga tgcgtgcgag
     1081 caggttcgcc ttctgggcgt cggtgatctc ctgcatcatg tcgtatagca cgatggcgtc
     1141 gtagcccttg gcccggcttt cggtgtactc atcactggcc gcgggctgga gggcctcgcg
     1201 ccactcgagg ccctcctcgg cactgaacat ggcgaagaat gcatcgcgct cgaagtcatg
     1261 cccgcccgtc acgatcaggg cccgtaccgt cgccgtgcgt ccgacggccg gtaccctcgc
     1321 cgccagcgcc gcgaccgcga cggcgccgac cagcaatccg ggagcgtacc gcatcgacct
     1381 acgcctcctc caacactcgc cggaaccacg ggaccgaacg ctccgtgggc gcgtctccgc
     1441 tgacctcgag cgagcagact acgttcttgc catgctcctt gagcatgcga acgcactgcc
     1501 ggatgccctc tgcgttcgct ccctcaccga tggccgccga gctggcggcg atgccggtcg
     1561 actcgccgcg gagtgctgcc gccagctcgt ggctcacttc cttgatgtgc atgtggtgca
     1621 cgtggtcgat gacctggcgg aggaagtccg gtgggttgtg accggcgatg aacgtgttac
     1681 ccgtgtcgaa gttcacgccc atcatcggcg accgcgtctg ggtgacgatc cgcagcagcg
     1741 agtcggggcg ctgggtcagt actccatgcg gctcgacaca gacatacacg ccatggcgct
     1801 cggccgaggg gaccacgcag tcgaagtggt acttgtagac gcccatccac tcgtcgagcg
     1861 agtacgtgtc aggaatgtgc tcgctgtccg tagtggcgat ggcgctacac ccgagcaccc
     1921 gtgcgaagcg aatggccttg atcatgtagt cgatgcactg gtagctccag atcggccagt
     1981 gggcgctcag gcacgagcag cgcaggccgt gttcgtcgat caggtccttg agcacgtacg
     2041 ggtcgctctc gagactgact gatggcgaga actgctggcc ctcaaagaag tccaggccgg
     2101 tgatc
//
LOCUS       DAOSPL010000903         2103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_52798_length_2103_cov_3.225098, whole genome shotgun sequence.
ACCESSION   DAOSPL010000903 DAOSPL010000000
VERSION     DAOSPL010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52798_length_2103_cov_3.225098"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..>2103
                     /locus_tag="PLQ54_15025"
     CDS             264..>2103
                     /locus_tag="PLQ54_15025"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOS94632.1"
                     /translation="MASRTGAGAPALLMMCMSLGVPANAAGRGLTPVPLTDVRLTDGF
                     WAPRLEVLRDVTIAHEIRMCEETGRIHNFRAAAGTEPGGFEGIFFNDSDVYKLIEGAS
                     YLYAIEGDAARRARIAEQIDPLIDAIAAAQQPDGYIYTFYTLTDPGLRWSDLPSMHEL
                     YCMGHLLEAGVAHFQATGSRRLLDVAIRAADCIDSVFGPGKNPGVCGHEEIELALVKL
                     ADATGDAKYLRLAEHFINARGYAHTEYDQNHIPVRDQRSVVGHAVRAMYLFCAAADVA
                     ARTGDAGLLEATRAIWDDLTFAKMYITGGIGPSGSNEGFTTAYDLPNETAYAETCAAI
                     GLVLLGHRMALVDADARYVDEAERALMNGALSGISLSGDRFFYVNPLGSRNGHQRVDW
                     FGCACCPPNILRVIPQVPGMLYATGERALYVQLYARSEARARVAGQAVALTQETDYPW
                     DGRIRLTVKPDMPAEFVVCLRKPGWCRSWTVRVNGGERPARVSDDGYIHLRERWSGQT
                     ITLDLDMPVERVEAHPMVQADVGRVALQRGPLVYCLEGVDNDRHVRDLYLPRDTSLRS
                     EFDGQLLGGVVKVRGTARRAAGEWDRRLYRTAELSGDVPFTAVPYYA"
BASE COUNT          330 a          738 c          702 g          333 t
ORIGIN      
        1 cgtgcggcgg ttcatcgagc tggctcgagc ggccggaggg agcggctgag cccgcgggca
       61 cccaccggtc aacttgatcc cgagcccagg ggccgccggg tcgcatcggg ccggtcgaag
      121 ggcagaggcc ggggaggatg ccggaagcgc ggcccgaagc atcacaccgg gcatttccca
      181 tgggtcgcac cggttcggcc atcatgctgc ccggtgcgcg agtggccgcc aggcatcccc
      241 aaggaggttg cgtacccgtg gtaatggcat cacggaccgg agccggtgcg ccggcgctcc
      301 tgatgatgtg catgtcgctg ggcgttccgg ccaatgcggc cgggcgagga ctcactcccg
      361 tgccgctcac cgatgtccgg ctcaccgacg gtttctgggc gcctcgcctc gaggtgctcc
      421 gcgatgtgac catcgcccac gagatccgga tgtgcgagga gacgggccgg attcacaact
      481 ttcgcgccgc ggcagggacc gagcccggcg ggttcgaggg catcttcttc aacgactccg
      541 atgtctacaa gctcatcgag ggagcctcgt acctgtatgc catcgagggc gacgcggccc
      601 gccgggcccg tatcgccgag cagatcgatc cgctgatcga cgccattgcg gccgcacagc
      661 agcctgacgg ctacatctac acgttctaca ccctcaccga cccggggctc cgctggtccg
      721 acctcccctc catgcacgag ctgtactgca tggggcatct gctcgaggcc ggcgtggcgc
      781 acttccaggc caccggcagc cgcagacttc tggatgtggc catccgcgcg gccgactgca
      841 tcgactcggt cttcgggcca ggcaagaacc ccggcgtgtg cggccatgaa gagatcgagc
      901 tcgccctggt caagctggcg gatgccaccg gcgacgccaa gtatctgcgc ctcgccgagc
      961 acttcatcaa cgcccgtggc tacgcccaca ccgagtacga ccagaaccat atccctgtcc
     1021 gcgaccagcg cagtgtcgtc ggccacgcgg tccgggccat gtacctcttc tgcgccgccg
     1081 ccgatgtggc cgcgcggacc ggcgatgcgg gcctgctgga ggccacccgc gccatctggg
     1141 acgaccttac atttgccaag atgtacatca ccggcggcat cggtccctcg ggcagcaacg
     1201 aagggttcac caccgcctat gacctcccca acgagacggc ttatgccgag acctgtgcgg
     1261 ccatcggcct ggtgctgctc ggccaccgca tggcactggt ggacgccgat gcccgatatg
     1321 tggatgaggc cgagcgcgcc ctgatgaacg gcgccctgtc cggcatcagc ctcagtggcg
     1381 accggttctt ctacgtgaat ccactgggca gccgcaacgg ccaccagcga gtggactggt
     1441 tcggctgcgc ctgctgcccg ccgaacatcc tgcgggtcat cccccaggtg cccggcatgc
     1501 tctacgccac gggcgaacgg gcgttgtacg tccagctcta cgcacggagc gaggcccggg
     1561 cccgggtggc gggccaggcc gtcgccttga cgcaggagac cgattacccc tgggatggac
     1621 gcattcgcct caccgtcaag cccgacatgc ccgccgagtt cgtggtgtgc ctgcggaagc
     1681 ccgggtggtg ccggtcgtgg acggtccgcg tcaacggcgg tgagcgtccc gcccgggtct
     1741 cagatgacgg ctacattcat ctgcgagagc gctggtccgg gcagacgatc acgctggatc
     1801 tggacatgcc cgtcgaacgc gtcgaagccc acccgatggt ccaggccgat gtgggacgcg
     1861 tggccctcca acgcgggccc ctggtctatt gcctggaggg ggtggacaac gaccggcacg
     1921 tccgagacct gtacctgccg cgcgacacct cgctccgcag tgagttcgat ggccaactcc
     1981 tcggcggagt cgtcaaggtt aggggaacgg cgcggcgcgc cgccggcgag tgggaccggc
     2041 gcctctaccg cacggccgag ctctcgggcg acgtcccctt cacggcggtg ccctactatg
     2101 cgt
//
LOCUS       DAOSPL010000904         2102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_52842_length_2102_cov_3.453835, whole genome shotgun sequence.
ACCESSION   DAOSPL010000904 DAOSPL010000000
VERSION     DAOSPL010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52842_length_2102_cov_3.453835"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /gene="ffs"
                     /locus_tag="PLQ54_15030"
     ncRNA           <1..257
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLQ54_15030"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            422..>2102
                     /locus_tag="PLQ54_15035"
     CDS             422..>2102
                     /locus_tag="PLQ54_15035"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOS94633.1"
                     /translation="MRKLTLGGAWALVLAAATVGQSAEGPWVARIPAEEAHPCLLFSL
                     DDLPQIQARLERAPHASWWHSIRASTNPVDQAFTWRVTGRAESAEAARLMLLRSNPTG
                     YHCCCGVADALQSYAETYDLLYGYEGLTATDHRVIRAKMAQACERLYLSALESGPGQH
                     PGNQRTRGICALGTAAIVLRGYTDAAHTPAKWLQRALDGIHDEANLCFWRDDGMFIEG
                     PDYSAFTLAVMMPFARYLERASGQWLFDDPRLANALRYLVYITQPDGACAALGTTNTS
                     MVLGALMLAVGAGPQEEQARIRWALEEWHGGLSGGVRELCLVDESVTPSVAGFATARA
                     FRASQEVAVRSEWSRNAVALWLKGKDPWIARGYPVYSHADAGSFVLHAYGELLAVDAG
                     YDHWVSHDLYPPELHNTLLVDGAGPGSETPGLLTAALSEGPVRGGEIESEFAGVRHRR
                     SMWLVDGEYVVAFDEVASEAEHTYDWQIHTPVTRGSAPVTLGQDRASWPGFDPMAEVT
                     GRVRLDAVWAGPVELARMEHSRWQPFSADPKTGSYDNEAIVARLRGRDASYL"
BASE COUNT          323 a          659 c          757 g          363 t
ORIGIN      
        1 taagcgggga gctagcggtg ccctgaaccc gcaatccgct ctagcggggc tgaatccccg
       61 atcgaggtgc acgatcttgg ggtctgaccg ttgtaagtgg tgttgacggt tcggtcctgc
      121 gcaacggcgt cctgtgaatc gggtcagggc cggaaggcag cagccctaag caatcgatgt
      181 ccgtgtccgc agccaaccgg gcccgagcag gctgcagcgg taacgcctgg gaccgtgtat
      241 cgacagaggg tgcacgatca ctgccctctt ccagttcgga gggtggctcc gagtgctcgg
      301 agccattctc ttcgtttccc ggcctcactc tgcccgcccc cgcagggctt ggcctatcgg
      361 ccgcgaactt ttcgccgtgc ggcggttgtc gctgcaacgg agccttcagc ggagatgtgg
      421 catgcgaaag ctcacccttg ggggcgcatg ggcgctcgtt ctcgccgcag cgacggtcgg
      481 ccagtcggcc gaggggccct gggttgcgcg cattccggcc gaggaggctc acccgtgtct
      541 gctcttctcg ctcgatgatc ttccccagat ccaagcgcgt ctggagcggg cgccccacgc
      601 gagctggtgg cactcgatcc gagcgagcac gaacccggtc gaccaggcgt tcacctggcg
      661 cgtcacgggg cgggccgagt cggcggaggc cgcccggctg atgctcctcc gcagcaaccc
      721 cacgggctat cattgttgct gcggagtcgc cgacgccctc cagagctatg cggagaccta
      781 cgatctgctc tacggctacg agggcctgac ggccacggat caccgggtca tccgcgccaa
      841 gatggcgcag gcctgcgagc ggctgtacct gtcggcgctc gagagcggcc ccggtcagca
      901 tccggggaac cagcggacgc gcgggatctg cgcgcttggc accgccgcga tcgtgctcag
      961 gggatacacg gacgcggctc acacacctgc caaatggctg cagagggcac tggatggcat
     1021 ccatgacgaa gccaacctgt gtttctggcg cgacgacggc atgttcatcg agggccccga
     1081 ctactcggcg ttcacgctgg cggtgatgat gccgtttgcc cggtacttgg agcgcgcatc
     1141 ggggcagtgg ctgttcgatg acccacgtct tgccaacgcc ctgcggtacc tggtctatat
     1201 cactcagccc gacggggcgt gcgcagccct ggggacgacc aacacctcga tggtgcttgg
     1261 ggcactgatg ctggccgtcg gtgcgggccc gcaggaggag caggcgcgga ttcgctgggc
     1321 gctcgaggag tggcacgggg ggctgtcggg cggtgtccgc gagttgtgcc tggtcgatga
     1381 gtcggtgact ccgtcggtgg cggggtttgc cacggcacgg gctttcagag ctagccagga
     1441 ggtggcggtt cgaagtgagt ggtcccgcaa cgcggtggcg ttgtggttga agggcaagga
     1501 tccgtggatc gcgcggggct acccggtcta cagccatgcc gatgccggca gtttcgtcct
     1561 ccacgcctac ggcgagctgc tggcagtgga cgcaggctac gatcactggg tgagccatga
     1621 cctctacccg ccggagctgc acaacacact gcttgtggac ggcgcagggc ctgggagcga
     1681 gacgccgggc ctgctgaccg cggcactgag cgagggaccc gtgcgtggcg gcgagattga
     1741 gagcgagttc gccggcgtgc ggcatcgacg gagcatgtgg ctggttgatg gcgagtatgt
     1801 cgttgccttt gacgaggtgg cctccgaagc ggaacacacc tacgactggc agatccacac
     1861 cccagtgacc cggggcagtg cgcccgtgac gctgggccag gaccgcgcgt cctggccggg
     1921 gttcgacccg atggcagagg tcacggggcg cgtgcgcctc gatgcggtat gggccgggcc
     1981 ggtcgagctt gcgcgcatgg agcactcgcg ctggcagcca ttcagcgcgg acccgaagac
     2041 gggctcctat gacaacgagg ctattgtcgc gcgtctgcgg gggcgtgacg cgagctacct
     2101 ca
//
LOCUS       DAOSPL010000905         2101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_52875_length_2101_cov_2.789345, whole genome shotgun sequence.
ACCESSION   DAOSPL010000905 DAOSPL010000000
VERSION     DAOSPL010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52875_length_2101_cov_2.789345"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1406
                     /locus_tag="PLQ54_15040"
     CDS             183..1406
                     /locus_tag="PLQ54_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94634.1"
                     /translation="MRNGTRHSQGQVLVISLVATVVMAFLGLGLMQLAQQSASAESTA
                     YRKAQSLWRVHGGVEYAVWNLLSTDVGSAYSGQAAPNPAYNWGYNGATLSYPLGGGSE
                     ADTSSVAITVSAPMASDPSIRAIRAETDVFGKTAAARHLIDVDRPPPALNLAILTEDS
                     LNVGGNTVVTSHSGAQGTLNADIHTNRNLSVDGSSVVNGFATASGTANVSGTVAPPVN
                     PGNLPPYFEGVSAVDIPNIDPASFASIADVTISNPKSIPMNAVLDFGGTPDDPTIVYL
                     NGDWQPAGHTYVRGNCVLVVNGKLKTAGLTYLEATAGGQFSVIASFVDLKGTSEIHGV
                     VYAREEFHSSGTAAIYGSAISKSYDPATGKTKTGSVFTGNFDVYYQPHGENLLPEPPT
                     AARLSYQTRGPGENW"
     gene            1511..>2101
                     /locus_tag="PLQ54_15045"
     CDS             1511..>2101
                     /locus_tag="PLQ54_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94635.1"
                     /translation="MNHRMPAAERFAGLDTGRTEGIGRPLPHNAHIHMPPNFSAFDTV
                     GQVVELAAQQHMALVCASNYYDHRVYDPFARMAFDAGVFPVFGLEIILFDEGLARCGV
                     RTNDPNNPGRVYLCGLGTPFIGHPPAGPDQTLGVIRRNDERRMRAMVERINEVLASRS
                     IGCRLAYEHAVDGLVRRHGVEPDSIVLQERHLAQALQ"
BASE COUNT          393 a          666 c          695 g          347 t
ORIGIN      
        1 cgggaacagg gagtctgatc gggggcaccc aggcggcgcc gttcgacgtg aagaccgtta
       61 cactgaccat cgagacgcag gggaagacct cgggcatcgg cgggagatct cagtccggga
      121 caacggtcac gaagacggtc cgactgcgga accacatgta gcccggagga ggaggggggg
      181 agatgagaaa cggcacaagg cattctcagg ggcaggtact ggtcatcagc ctggtcgcca
      241 ctgtggttat ggccttcctg gggctcggat tgatgcagct cgcccagcag tcggcttcgg
      301 ccgagtcgac tgcctatcgc aaggcgcagt cgctctggcg agtgcacggc ggcgtggagt
      361 atgccgtctg gaacctgctc agcactgatg tgggttccgc ctattcgggc caggccgctc
      421 caaacccggc ctacaactgg ggctacaacg gggcgaccct ttcgtacccg cttggaggcg
      481 ggagtgaggc tgatacgtcc tctgtggcga tcacggtatc ggcgcccatg gcatcggacc
      541 cgagcatcag ggccatccgt gccgagaccg atgtatttgg gaagacggca gcggctcgac
      601 acctgatcga cgtggaccgg cccccgcccg cgctgaacct cgcgatcctg actgaggact
      661 cgctgaacgt tggcggcaac acggtggtca ccagccattc gggtgcacag ggcacgctga
      721 acgccgatat ccacacgaac cgcaacctgt cggtggacgg gtcctcggtc gtcaatgggt
      781 tcgcgacggc gtcagggacg gcaaacgtga gtggcacggt ggcccccccg gtcaacccgg
      841 gcaacctgcc gccctacttt gagggggtgt cggcggtcga catcccgaac atcgacccag
      901 catcctttgc gagcatcgcg gacgtgacga tctccaaccc gaagagcatc ccgatgaacg
      961 ccgtgttgga cttcggcggc acaccggacg accccacgat cgtgtacctg aacggcgact
     1021 ggcaacccgc cgggcatacg tatgtgaggg gcaactgcgt cctcgtcgtg aacggaaagc
     1081 tgaagacggc agggctgacg tatctggagg ccacagcggg aggccagttc tcggtgatcg
     1141 cgagcttcgt ggacctcaaa ggcacctcgg agatccatgg cgtggtatac gcccgggagg
     1201 agttccatag ctcgggcacc gcggccatct atgggtccgc gatctcgaag tcctacgacc
     1261 cggcgaccgg caagaccaag accggatccg tgttcacggg gaacttcgac gtgtactacc
     1321 agccgcacgg ggagaacctg ctcccagagc ctccgacggc cgcacggttg tcgtaccaga
     1381 cgcgaggacc aggtgagaac tggtaggggg aaccggagcg ccacgagcct tgtagtatca
     1441 tggagcgccg ctttcccgcg gcgcttcgtc tcgtacgggg cccgatactg tcactgcgtg
     1501 gtgagtcttc gtgaaccacc gaatgcccgc cgccgagcgg tttgccggac tggacacggg
     1561 tcgaacagaa ggcattggtc gtcccctacc tcacaatgcc cacatccaca tgccgccgaa
     1621 cttctcggca ttcgatacgg tggggcaggt cgtcgagctg gcggcgcaac agcacatggc
     1681 cctggtgtgt gccagcaact actacgacca tcgagtctac gaccccttcg cccgcatggc
     1741 tttcgatgcg ggcgtgttcc ccgtgttcgg gctcgagatc atcctcttcg acgagggact
     1801 cgcgcggtgc ggggttcgga cgaacgaccc caacaacccg ggccgcgtat acctgtgtgg
     1861 gctgggcacg ccgttcatcg gccacccgcc ggccggtccc gaccagacgc tgggagtgat
     1921 ccgccggaac gacgaacgcc gtatgcgggc catggtcgag cggatcaacg aggtcctcgc
     1981 cagccggtca atcggctgcc gtctggccta tgagcacgcc gtggatgggc tcgtgcgacg
     2041 gcacggcgtc gagcccgact ccatcgtcct gcaggaacgg catctggctc aagccctgca
     2101 g
//
LOCUS       DAOSPL010000906         2099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_52933_length_2099_cov_3.041585, whole genome shotgun sequence.
ACCESSION   DAOSPL010000906 DAOSPL010000000
VERSION     DAOSPL010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52933_length_2099_cov_3.041585"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PLQ54_15050"
     CDS             <1..686
                     /locus_tag="PLQ54_15050"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOS94636.1"
                     /translation="KAYRNLRAFLSFCVQDDLLPRSPMARLTAPSLAADQREPLSGEE
                     VKRLLLGARRSGLPDRDAALVLLLLDTGLRASEVCGLTIGHALSAEFSAEVVGKCNKR
                     RAVFWSPETAKALRRHLRARGCIDPAAPLFVTRTGRPFTAANVHEVVSDAGERGAVGR
                     SVFPHLLRHTAALALLRGGANVLEVQRILGHSDLSMLRRYVLETREDLQRAHAAASPV
                     RALAGGGRR"
     gene            683..925
                     /locus_tag="PLQ54_15055"
     CDS             683..925
                     /locus_tag="PLQ54_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94637.1"
                     /translation="MRVTLRNEFHRTRAEVVVPTLPHTLSASQYRRALRALCPCRAGV
                     CQCGGLRGPQSVGVELTADANGRPTWRLSAREELAP"
     gene            922..1191
                     /locus_tag="PLQ54_15060"
     CDS             922..1191
                     /locus_tag="PLQ54_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94638.1"
                     /translation="MNTDRLTIPVDRDLPYRLHVALWREEPTEDERAYLAECLDSYCE
                     RDAHFTPARREARLAARAGAPVHGADRPRPHALPAQPMDAPGVQT"
     gene            1324..>2099
                     /locus_tag="PLQ54_15065"
     CDS             1324..>2099
                     /locus_tag="PLQ54_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94639.1"
                     /translation="MARRRSPELVTPLHPSSYSFGGRTVVRTRAGETRTVGPLPAAPW
                     PALPERYTVGSVVSQCQEPDRCDLWLAGAPDPREGYAGPDHVCSGVAATSRWAYAAER
                     VPAMGSGCEPRDTIDCAMSNVATTADEFGNGWHRDACERLGAKRALCPPLLVRSEPRQ
                     HGFVAFREVASRRGLTRVYVFGLIHWEYFGDAPEDDGYDEDGNARCIWVTDMRPVWDS
                     TATLYDGPETTEAHRAARRVCRWFDARLCGRPTSDGRPPR"
BASE COUNT          300 a          761 c          742 g          296 t
ORIGIN      
        1 acaaagcgta ccgcaacctg cgggcgttcc tgagcttctg cgtgcaggac gacctcctgc
       61 ctcgctcgcc gatggcccgc ctcacggccc cctcgctcgc cgccgaccaa cgcgagccgc
      121 tgagtgggga ggaggtgaag cggctgctgt tgggggcgcg gaggtcgggg ctccccgacc
      181 gcgacgcggc cctggtgctg ctcctgctcg acacggggct gcgtgcctcg gaggtatgcg
      241 ggctgaccat cggccacgcc ctgagcgcgg agttctcggc cgaggtcgtg ggcaagtgca
      301 acaaacggcg agccgtgttc tggtcacccg agacggccaa agccctccgc cggcacctgc
      361 gtgcccgtgg gtgtatcgac cccgccgcgc ccctgttcgt gacccggacc ggccggccat
      421 tcactgcggc caacgtgcac gaggtcgtgt cagacgccgg cgagcggggc gccgtcggca
      481 gatcggtgtt cccgcacctg ctgcgccaca cggcagccct ggccctgctc cgtggtggcg
      541 cgaatgtgct agaggtgcag cggatcctcg ggcattctga cctgtccatg ctccgtcggt
      601 acgtgctgga aacgcgagag gacctgcaac gggcccacgc ggccgcgtcg cccgtgcgcg
      661 ccctggcagg gggtgggcgc cgatgagggt aacgctgcgg aacgagttcc accggacccg
      721 ggccgaggtg gtcgtgccca cgctgccgca caccctgagt gcgtcgcagt accgtcgtgc
      781 cctgcgagcc ctgtgcccct gccgtgccgg tgtgtgccag tgtggcgggc tcagaggacc
      841 gcagagcgtg ggcgtcgagc tgaccgccga cgccaatggg cgcccaacat ggcgcctgag
      901 cgcccgagag gagctagccc catgaacacc gaccgcctga cgatccctgt cgaccgggac
      961 ctgccgtacc gcctgcacgt ggccctatgg cgcgaggagc cgaccgagga cgaacgcgcc
     1021 tacctggctg agtgcctgga ctcctactgc gagcgggacg cgcacttcac gcccgcccgg
     1081 cgggaggccc ggctggcggc ccgcgcgggt gcccccgtgc acggcgccga ccgacccaga
     1141 ccccatgcac tcccggcgca acctatggac gccccgggcg tccagacgta gagacccgac
     1201 aacacaacgt gagcaccggg ccgcctcgcg aacggcccgg cggggggcgc ccaccgtgcc
     1261 tccggcgccc cccacgctcc cgactcccgc tcgacccaga ggaggcacgg aggaggcaca
     1321 accgtggcgc gtagacgctc gcctgagcta gtgacccctc tgcacccctc gtcctactcg
     1381 ttcggcggcc gcaccgtggt gagaacgcgc gccggcgaga cgcgaaccgt cggccctctg
     1441 cctgccgccc catggcctgc cctgccagag cgatacacgg tgggcagcgt ggtgagccag
     1501 tgccaggagc cagaccggtg cgacctctgg cttgccgggg ctcccgaccc acgcgagggc
     1561 tatgccggcc cagaccacgt gtgctccggc gttgccgcaa cgagtcggtg ggcctatgcg
     1621 gccgagaggg tcccggccat gggcagtggg tgcgagcccc gcgatacgat cgactgcgcc
     1681 atgtccaacg tggcgacgac ggctgacgag ttcggcaatg gctggcatcg tgacgcgtgc
     1741 gaacggctgg gcgccaagcg ggctctgtgc ccccccctcc tggtgcggag tgagccgcgc
     1801 caacacgggt tcgtcgcgtt ccgcgaggtc gcgtcgcgtc ggggccttac gcgcgtctac
     1861 gtgttcgggc tcattcactg ggagtacttc ggcgacgcac cggaggatga cgggtacgac
     1921 gaggacggca acgcgcggtg tatctgggtg accgatatgc gcccggtgtg ggactcgacg
     1981 gcgaccctct acgacgggcc cgagacaaca gaggcccacc gtgctgcccg ccgcgtgtgc
     2041 cgttggttcg atgcccgcct gtgtgggcgc ccgactagtg acggacgccc gccccgctt
//
LOCUS       DAOSPL010000907         2095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_53067_length_2095_cov_3.792647, whole genome shotgun sequence.
ACCESSION   DAOSPL010000907 DAOSPL010000000
VERSION     DAOSPL010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53067_length_2095_cov_3.792647"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PLQ54_15070"
     CDS             <1..1020
                     /locus_tag="PLQ54_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94640.1"
                     /translation="LALGDRLRQAITYDSGLRLWINWSPEPWEVQGRTLPQWGFLALG
                     PDTEVRTTVEGGVYSDYADCPEYLFVDARNRLNMPYAGGGKDIEPRLGRFEYLGANRV
                     RLSYEWAINDTLDRDYHVFVHFTNAQQTNNAEGIAFQQDHDTALPTSQWQEGQTLVDG
                     PYEITIPEDQPFDSYDVTIGLHSGERVPLRGLSPSGGKILIGRMLVTRENGRVTGVRL
                     GSIDADRERTAAGLVDFSAHLNPVGTMVDFGAVRTDGSLKIEKGEGKLLVLPYPRGVE
                     AAVALDVGAMLGRPGSVRPGDVRIQALAAGTRAPMGDVPCRAEGDRCVFTVGAPGVGA
                     YSVTW"
     gene            1040..1492
                     /locus_tag="PLQ54_15075"
     CDS             1040..1492
                     /locus_tag="PLQ54_15075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOS94641.1"
                     /translation="MIDHLSRACVYYGLGRGIRDALRFLAQADLAAVSPGRHELSGGS
                     YAMVSEYETKAAEGALYEAHRRFVDVQFVVSGSERIGYAPLEALREAQPYDDERDVVK
                     LEGEGDPVLLTAGQFAVLWPGDGHMPGIHAGDRPAHVRKVVIKVPVAG"
     gene            1504..>2095
                     /locus_tag="PLQ54_15080"
     CDS             1504..>2095
                     /locus_tag="PLQ54_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94642.1"
                     /translation="MYPFGFRSVYVTDWAAPDDASQARLGRICEGFGIDDARVVGAAE
                     LAELVEAQGWNAPVRTGALPDPRERDLVLNAYRFDGSAERDIERYPALRSWTMGSEKP
                     WSWRDAALEGAGSGSVCQSAWELHSIYGCLHACRYCHVGRLVNMMLNLEQIRDRLHEL
                     FALLPSQRLFKYDNQTDTLCFEPEYGASETFVPFFGE"
BASE COUNT          343 a          678 c          716 g          358 t
ORIGIN      
        1 ttggccctgg gcgaccgcct gcgccaggca atcacctacg atagcgggct gaggctatgg
       61 atcaactgga gccccgagcc gtgggaagtc caaggccgca cgctccccca gtggggcttc
      121 ctggccctcg gcccggacac cgaggtgaga accacagtcg agggcggcgt gtactccgac
      181 tatgccgact gtcccgagta cttgttcgtc gatgcccgca atcggctgaa catgccctat
      241 gcgggaggcg gcaaggacat cgagccgaga ctgggacggt ttgagtactt aggcgcgaac
      301 cgcgtccggc tcagctatga atgggccatc aacgacacgc tcgaccgcga ctaccatgtg
      361 ttcgtccact tcacgaacgc gcagcagacc aacaatgctg aggggatcgc cttccagcag
      421 gatcacgata cggcgctgcc caccagccag tggcaagagg gtcagaccct ggtggacggg
      481 ccgtatgaga tcaccatccc ggaggatcaa ccgttcgaca gctacgatgt caccatcggc
      541 cttcactcgg gcgaacgcgt gcctctcaga ggtctctcac ccagcggcgg caagatcctc
      601 atcggccgaa tgctggtcac ccgtgagaac ggccgagtga ccggcgtccg cctcggcagc
      661 atcgacgccg accgagagcg caccgcggcc ggcctggttg acttctccgc tcacttgaac
      721 cccgtgggca cgatggtcga cttcggagcc gtgcgcaccg acggctcgct caagatcgaa
      781 aagggcgagg gcaagctcct ggtgctcccc tatccgcgcg gtgtagaggc ggccgttgcc
      841 ctcgacgtcg gcgcgatgct cgggcggccg ggttcggtca ggcctggtga tgtgcgcatt
      901 caggcgctgg cggcgggaac gcgcgcgccc atgggcgacg tgccctgcag ggccgagggc
      961 gaccgctgtg tcttcaccgt cggcgcacct ggtgtgggcg cctactcggt gacgtggtag
     1021 agggaggcgt ttcggcttgg tgatcgacca cctcagccgg gcatgtgtct actacggcct
     1081 gggacgcggg attcgcgatg cgcttcggtt cctggctcag gccgatctcg ccgcggtgtc
     1141 gccgggccgg cacgagctct cgggcggctc gtacgccatg gtcagtgagt atgagaccaa
     1201 ggccgccgag ggagctctat acgaggcaca ccgccggttc gtcgatgtcc agttcgtcgt
     1261 cagcggctcc gagcggatcg gctacgcgcc actcgaggca cttcgtgagg cccagcccta
     1321 tgacgacgag cgcgacgtgg tgaagctcga gggcgaaggc gatccggtcc tgctgacggc
     1381 cggccagttc gctgtgctct ggccggggga tggccacatg ccaggcatcc atgcgggcga
     1441 tcggccggct cacgtccgga aggtcgtcat caaggtgccg gtggccgggt aggggatctg
     1501 cgcatgtacc cgtttggttt ccgttcggtc tacgtgactg actgggctgc gcccgatgac
     1561 gccagtcagg cgcgtctggg gcgcatctgc gagggctttg gtatcgacga cgcccgggtc
     1621 gtcggcgccg cggagcttgc cgaactcgtc gaggcccaag ggtggaacgc gcccgtgcga
     1681 accggggcgc tacccgaccc gcgcgaacga gacctggtgc tgaacgccta ccgcttcgat
     1741 ggcagcgccg agcgggacat cgagcggtac ccggctctcc ggtcgtggac catggggagc
     1801 gagaagccgt ggagctggcg cgatgccgcg ctcgagggcg ccgggtcggg ctcggtctgc
     1861 cagtcggcct gggagctcca ctcgatctat ggctgcctgc acgcctgccg ctactgccac
     1921 gtcggccgcc tcgtgaacat gatgctgaac ctcgagcaga tccgtgaccg gctgcatgag
     1981 ctcttcgctc tgctacccag tcaacggctg ttcaagtacg acaaccagac tgacacgttg
     2041 tgcttcgagc ccgagtacgg agcctcagag accttcgtgc cattcttcgg cgagc
//
LOCUS       DAOSPL010000908         2094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_53115_length_2094_cov_2.507111, whole genome shotgun sequence.
ACCESSION   DAOSPL010000908 DAOSPL010000000
VERSION     DAOSPL010000908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53115_length_2094_cov_2.507111"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..1314)
                     /locus_tag="PLQ54_15085"
     CDS             complement(61..1314)
                     /locus_tag="PLQ54_15085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS94643.1"
                     /translation="MTTPLPGRRRLAVLALSVIALSIYHVTAIPILAPVLECQFGLSP
                     KRLGLLLSVSMVGGTLGAAAAGWLADRWGRVRILSVATGVTAAGYYLCAAPSVLAVFY
                     AGLALAGVGCSAANVACGGLLPALFPERRRGSFTTLLVTGAAAGILLPYPVAYIQGLH
                     EAGRMSFGLMMAAPFVLVGLLLTGLALWLMRFASPVDRVASLSTAQGAADTPRLRRGW
                     ALNGVPLTAVGIVAFLAAAHGLSDNVLYSWITRHLTASFDEHPLPPGWILSFYNAAYL
                     AGRLGLAMLPDQVGRRTLTIVPGLIAGPILFAAIQSDTYLLTFGGYVLACLFSGLEYP
                     ALMGLVAQRFPDRFATVFGLAGTTTLLSVPALWGVGKWAEVAGSTRPPLSAAAGGYVV
                     FALVAALWLVADLRSKEQAVDAVSC"
     gene            complement(1322..>2094)
                     /locus_tag="PLQ54_15090"
     CDS             complement(1322..>2094)
                     /locus_tag="PLQ54_15090"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HOS94644.1"
                     /translation="PCFRILREIPVLSGGWDSRPWHGNKALVLHSRTPEKFEQHCRDA
                     RAFLDATEPDPARRMCIVEAWNEWGEGSYIGPHREFGFGYLDAIRNVFTRAAREHVDL
                     TPKDVGLGPYDLPPVAVRAEWDFDDGTDQGWGSTMGLGEVRVVDGVLTSTTTTSDPAF
                     FGPQTHARASRYPFAVIRMRVSHSDEAQLFWSTTRGAATEGTSVRFEVIADGQFHDYR
                     IRVGDTASWRGMITSLRLDPANQPGVQVEVDSIRLEMD"
BASE COUNT          392 a          743 c          691 g          268 t
ORIGIN      
        1 cctcgagcgc gacgcctagc ggcgccgcag agcaaagcgg gcggaggccg gcgccaccga
       61 tcagcatgac accgcgtcga ccgcctgctc cttgctccgc aggtccgcca ccagccacag
      121 tgcggccacg agggcgaaaa ccacgtaccc acccgcggcg gccgagagcg gtggacgtgt
      181 gctgcccgcc acctccgccc acttcccgac gccccacagc gcgggcacgc tcagcagagt
      241 ggtggtcccg gctaggccga agactgtggc gaaacgatcc ggaaaccgct gcgccacgag
      301 ccccatgagg gccgggtatt cgaggccgga gaacagacac gccagcacat agccgccaaa
      361 ggtcagcagg tatgtgtcgg actggatggc cgcgaacaga atgggacccg cgatgaggcc
      421 cggcacgatg gtcagtgtcc gacggcccac ctgatcgggc agcattgcga gtcccagtct
      481 cccggccagg taggctgcgt tgtagaacga gaggatccag cccggcggca gggggtgctc
      541 gtcgaaggac gccgtcaggt gtcgcgtgat ccatgagtac agcacgttgt ccgacaggcc
      601 atgggcggca gccagaaacg caacgatacc caccgccgtc agcggcacac cgttcagggc
      661 ccagccccgc cgcaagcgag gcgtgtcagc agccccctgg gccgtgctca gcgatgccac
      721 ccgatcgacc ggcgaggcga agcgcatcag ccagagcgcg agcccagtca gcaggagccc
      781 caccaggacg aaaggggcgg ccatcatcag gccgaacgac atccggccgg cctcgtgaag
      841 gccctggatg tacgccaccg ggtaaggcaa caggataccg gccgccgccc ccgtgaccag
      901 cagggtcgtg aagctccctc ggcgccgctc gggaaacaag gccggcagta ggcccccgca
      961 tgccacattg gcggcgctgc accccacccc tgcgagagcc aggccagcat agaagacggc
     1021 cagcactgag ggcgcggcgc acaggtaata gccagccgcc gtgacccccg tggccaccga
     1081 caggatccgg acccgccccc agcggtcagc cagccatccg gccgctgccg cgccgagcgt
     1141 cccacccacc atgctgaccg acagcagtag gcctagccgc ttgggcgaga ggccgaactg
     1201 gcactcgagc accggcgcga ggattgggat cgcggtcaca tggtagatgc ttagcgcaat
     1261 gaccgacagg gccagaacgg ccaggcgccg ccggccggga agaggcgtgg tcatcggcga
     1321 actagtccat ctcgaggcgg atcgagtcca cctcgacctg gacgcctggc tggttcgccg
     1381 gatccagccg cagcgacgtg atcatgcccc gccagctggc cgtgtcgccc acgcggatgc
     1441 ggtagtcgtg gaactgcccg tcggcgatga cctcgaatcg cacgctggta ccctcggtgg
     1501 cggcacctcg ggttgtgctc cagaacagct gcgcctcatc gctgtggctc acccgcatgc
     1561 ggatgacggc gaacgggtac cgactagccc tggcatgagt ctgggggccg aagaaagccg
     1621 gatcgcttgt ggttgtcgtc gaggtgagca ctccatcgac gacccgcact tcgcccaggc
     1681 ccatggtcga gccccagccc tggtcggtgc cgtcgtcgaa atcccactcg gcccggaccg
     1741 ccacgggagg tagatcatac ggtccgagac ccacgtcctt cggcgtcagg tccacatgct
     1801 cccgagcagc ccgggtgaac acgttgcgga tggcgtcgag gtagccgaaa ccgaactcgc
     1861 ggtgcgggcc gatgtacgat ccctcgcccc actcgttcca cgcctcgacg atgcacatgc
     1921 gccgcgccgg atccggctcg gtggcatcca gaaacgccct ggcgtcgcga cagtgctgct
     1981 cgaacttctc gggcgtccgg ctgtgcagaa ccagagcctt gttgccatgc cacggacgcg
     2041 agtcccaccc gccgctcagc accgggatct cgcggaggat ccggaagcac gggc
//
LOCUS       DAOSPL010000909         2093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_53139_length_2093_cov_3.222767, whole genome shotgun sequence.
ACCESSION   DAOSPL010000909 DAOSPL010000000
VERSION     DAOSPL010000909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53139_length_2093_cov_3.222767"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /locus_tag="PLQ54_15095"
     CDS             <1..1535
                     /locus_tag="PLQ54_15095"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOS94645.1"
                     /translation="GSMKLSADFAGGGAYVGAWKDMPDLQGRFLTGIRLWVKSRGVSQ
                     IGIRLADRSGQCHQATRVAVQPDDQWHELVLPLTDLVGGEHWGGANDGKWHGPPTGFG
                     LNIGRDGLAGGTAGTLWFDDFSCTVSNTAPGQPTVLAGVLSQPSCRPGYGFDLTFRWL
                     AEPMERDFTVFVHFVGPGGDTVFQGDHEPPLGTAVWTGAVEYQHTVVVPADAPAGEYR
                     IMVGLYDHAAAGRGWDHQTLRVGEGVRIQDDGTTYEIGRFTVDPAAPVPKLPPPTLNL
                     EGYHLTFSEEFDGPLDVSAWGPGTRWIAHTPYAGDFGDAPFADPEPGFPFTVENGVLR
                     IEARKDDRGWRAGLLSSVDSKGRGFSQQYGYFEMRAMFPKGPGTWPAFWLLGVPELVN
                     RGKVTQIEIDVVEQYGIAPNALHTTVHLWYPGGKHWAEGKPSIVEGMTDDFHTYGVSI
                     DEADIVFYYDGVELRRMNTPEEAKVPLYVLIDLALGGGWPIDQTPNPSFMYVDYLRAY
                     AK"
     gene            complement(1545..>2093)
                     /locus_tag="PLQ54_15100"
     CDS             complement(1545..>2093)
                     /locus_tag="PLQ54_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94646.1"
                     /translation="PTGGGGFSSGTEIGLGVFWGGFPEKSYGMIELLAHEATHSWVLP
                     FPEPMWNEGIASYVGILAGRGMGEAEAADETLKGWIDNARRDDPDFSKLDIAHGTGVP
                     HSVAMAKPMWIFEQLRTERPDILARYFQAKRQLIDPGKVDDYTADDAVAVLSIAMERD
                     LFPWFKSLGITVDRAKTALPVP"
BASE COUNT          351 a          685 c          705 g          352 t
ORIGIN      
        1 cgggcagcat gaagctctcg gccgacttcg cgggaggcgg cgcgtacgtc ggggcctgga
       61 aggacatgcc cgacctccag ggccgtttcc tgacgggtat ccgcctgtgg gtgaagtcga
      121 ggggcgtttc gcagatcggg atccggctgg ccgaccgctc gggccagtgc caccaggcca
      181 ctcgcgtggc ggttcagccg gatgaccagt ggcacgagct cgtactgcct ctcaccgacc
      241 tggtgggtgg cgagcactgg ggcggggcca acgacggcaa gtggcacggg ccgcccacgg
      301 gattcgggct gaacatcggc agggacgggc ttgccggggg cacagcgggt accctatggt
      361 tcgacgactt ctcatgcacc gtctccaaca ccgcgcccgg acagccgacc gtccttgccg
      421 gcgtgctctc gcaaccctca tgtcgcccgg ggtatgggtt cgatctgacg ttcagatggc
      481 tcgccgagcc tatggagcgc gacttcaccg tgttcgtcca cttcgtgggg cctggaggag
      541 acaccgtgtt tcagggggac cacgagcccc ccctcggcac ggccgtgtgg accggagccg
      601 tggagtacca gcacacggtt gtcgtgccag ccgatgcacc ggccggcgag taccgcatca
      661 tggtgggcct ctacgaccac gcggccgccg ggcgcggctg ggaccatcag acacttcggg
      721 taggcgaggg cgtcaggatc caggacgacg ggacgacgta cgagatcggc aggttcaccg
      781 tcgacccggc agcccctgtg cccaagctcc ctccgccaac actgaacctc gaaggctacc
      841 acctgacctt ctcggaggag ttcgacggcc cactcgacgt gtcggcgtgg gggccgggga
      901 cgcgctggat tgcccacacg ccctacgcgg gcgacttcgg cgatgcgccg ttcgccgatc
      961 ccgagcccgg gttcccgttc accgtggaga acggtgtgct ccgcatcgaa gccaggaagg
     1021 acgaccgcgg ctggcgggcg ggcctgctct cgtcagtcga ctcgaagggt cgcggcttct
     1081 cgcagcagta cggctacttc gagatgaggg ccatgttccc caaagggcca ggcacgtggc
     1141 ccgccttctg gctgctgggc gtgcccgaac tggtgaaccg cggcaaggtt acgcagatcg
     1201 agatcgacgt ggtcgagcag tacggcattg cgcccaatgc cctgcacacc acggtacacc
     1261 tctggtatcc cggcgggaag cactgggccg aggggaagcc gtccatcgtc gagggcatga
     1321 ccgacgactt ccacacgtat ggtgtatcga tcgatgaggc cgacattgtc ttctactatg
     1381 acggagtcga gctgcgccgg atgaacaccc ccgaggaggc gaaggtgccg ctgtacgtac
     1441 tcatcgatct ggccctcggc ggcggttggc ccatcgacca gacgccaaac ccgtcattca
     1501 tgtacgtcga ctacctgcgg gcgtatgcga agtaggctcg cgcctcaggg caccggcagc
     1561 gccgtcttcg cgcgatccac ggtgatcccc aatgacttga accaggggaa caggtcgcgc
     1621 tccatggcga tgctgagcac cgcgacggca tcgtcggccg tgtagtcgtc caccttgccg
     1681 ggatcgatga gctggcgctt ggcctggaaa taccgggcca agatgtccgg cctctcggtg
     1741 cggagctgct cgaagatcca catcggcttc gccatggcaa cgctgtgcgg cacgccggta
     1801 ccgtgggcga tatccagctt ggagaaatcg ggatcgtctc tgcgtgcgtt gtcgatccag
     1861 cctttgaggg tctcgtcggc cgcttcggcc tcacccattc cgcgtccggc gaggatgccc
     1921 acgtaggacg cgataccttc gttccacatg ggctcgggga agggcagcac ccacgagtga
     1981 gtggcctcgt gggccagcag ctcgatcata ccgtatgact tctcggggaa gccgccccag
     2041 aagacaccga ggccgatctc tgtcccgctg gagaacccac cgccgccggt ggg
//
LOCUS       DAOSPL010000910         2093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_53140_length_2093_cov_3.217370, whole genome shotgun sequence.
ACCESSION   DAOSPL010000910 DAOSPL010000000
VERSION     DAOSPL010000910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53140_length_2093_cov_3.217370"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLQ54_15105"
     CDS             <1..587
                     /locus_tag="PLQ54_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94647.1"
                     /translation="GGTELAWSPAGDVVAFVRAGDLWTCRPDGTNVTRVTNTGVTERH
                     PSFSPDGKSLCFHLDEADLDSGEIGTVGVDGSGWKLLTSGVNASSTAWSPLGGEIAYH
                     VDLDPETEDKALFFADATTGAVTEVSLEGDEDWADSWPAWSPDGRHIVFVREDMLAGA
                     SRGVDLWTVEREDLTKLEQLTKTPAVSESTPVWR"
     gene            711..>2093
                     /locus_tag="PLQ54_15110"
     CDS             711..>2093
                     /locus_tag="PLQ54_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94648.1"
                     /translation="MSRSAGRQRGVRGYVALGFLAAAILAGCDDSGIVLDPTGQPTTG
                     SLILTMEWPPNLVSTRVLPGFAETMGVTVSGTGPSGPFTVSRQATREDVHNGRASVAL
                     HLPAGSGHIVTVVARDADGNPVGVGHATVSIPAGGSALASVSLAPLGTTGSTALPLVS
                     SLSGRVTAWTGSFAGESDPPPGVVAVDLFGEGGAAEIARVTFVMHNAGELWIVQCSGV
                     VEGNRVYAEAASISFLRRIPLAGGGVCDYGGWTAAFEGSLASQDLNLVEVDQAIEAGG
                     ATIDLAVAGEWAPGRTLTGTVVARAPGEHRLSGTFVCGTSGTGENGLPSVPGLVSQRG
                     IIIDGFEGNFAPSGNGGTVTTSGASVRQLTFTGLNAMIFVDVFALGETLAPQTVVTMF
                     FHNATELWQLRGVAPVDPSGIAGLWVAPQQAIDYFSGFTNEVGGYTTDFTGRVLDFSL
                     VSGGVQDPLDP"
BASE COUNT          317 a          665 c          761 g          350 t
ORIGIN      
        1 cggggggcac cgaactcgca tggtcacccg ccggcgacgt ggtggccttc gtccgcgcgg
       61 gtgatctgtg gacatgccgc cccgacggga ccaacgtgac acgggtcacg aacacaggcg
      121 tgactgagcg acacccgagc ttctcgcccg atggcaagag cctgtgcttc cacctcgacg
      181 aggccgactt ggacagcggc gagatcggga cggtgggcgt cgacgggtcc ggctggaagc
      241 ttctgacatc gggagtgaat gcatccagca ccgcctggtc tccgctgggc ggcgagatcg
      301 cgtaccacgt ggacctcgac ccggagaccg aggacaaggc gctgttcttc gccgacgcta
      361 ccaccggggc cgtaaccgaa gtgtcgctgg agggcgacga ggactgggcc gactcctggc
      421 cggcctggtc accagacggg aggcacatcg tgttcgtccg ggaggatatg ctcgccgggg
      481 cgtcgcgcgg tgtcgacctc tggaccgtgg agcgtgaaga cctgacgaag ctggaacagc
      541 tcaccaagac gccggctgtg agcgagagca ctccggtttg gcgctagccc gtcgctgatc
      601 ggtagattcg cgagaacacc ggcctcgtca ttgcgtccaa ggactagagg tcctacggcc
      661 cggcggcact tcggtccgtg gccgccgact tgcgtgacag aggcgtgcgt atgagcagat
      721 cggcagggcg tcagcggggc gtccggggtt acgtggcgtt gggctttctg gctgccgcca
      781 tcctggcggg ctgtgacgac tcagggatcg tgctcgaccc cactgggcag ccaacgaccg
      841 ggtcgctgat cctcacgatg gagtggcccc cgaacctcgt cagcactcgg gtgcttccgg
      901 ggttcgccga gaccatgggc gtcactgtca gcgggaccgg tccgtcggga ccgttcaccg
      961 tgagccgaca ggccacccgc gaagacgtcc acaacgggcg tgcgtccgtc gccctgcacc
     1021 tgcccgccgg gtccggtcac atcgtgaccg tggtggcgcg cgacgcggac gggaacccgg
     1081 tgggcgtggg acatgcgacg gtgtcgatcc cggccggcgg gagcgcgctc gccagcgtct
     1141 cgctggcgcc cctggggaca accgggtcca cggccctgcc cctggtcagt tcgctgtcgg
     1201 gtcgcgtcac ggcatggacg ggaagctttg cgggcgagtc agaccctccg cccggcgtgg
     1261 tggccgtgga cctcttcggc gaggggggcg cggcggagat tgcccgggtg acgttcgtca
     1321 tgcataacgc gggcgaactg tggattgtgc agtgctcggg cgttgtcgag ggcaatcgtg
     1381 tctatgccga ggcggccagc atctcgttcc tgcgccggat accattggcc ggcggaggcg
     1441 tgtgtgacta cggcggatgg acggcggcct tcgagggctc actggcgtcg caggacctca
     1501 acctcgtcga agtcgaccag gccatcgagg cgggcggtgc gacgatcgat ctggccgtgg
     1561 cgggcgagtg ggctccaggc agaaccctga ccgggactgt cgtcgcccgg gcgcccggcg
     1621 agcatcgcct ctcgggcaca ttcgtgtgcg ggacgtccgg tacgggagag aacgggctcc
     1681 cgagtgtgcc cgggctggtc agccagcgcg ggatcatcat cgatgggttc gagggcaact
     1741 tcgcgcccag cggcaatggc ggcacggtga cgacgtctgg cgcgtcggtc cgccagctca
     1801 cgttcactgg gctcaacgcg atgatcttcg tggatgtgtt cgccctgggc gagactctgg
     1861 ctcctcagac ggtggtcacg atgttctttc acaacgccac cgagctgtgg cagctccgcg
     1921 gggtggctcc cgtggacccc agcggcatcg cggggctttg ggtggcgcct cagcaagcca
     1981 ttgactactt ctctgggttc accaacgagg tcgggggata caccacggat ttcacgggca
     2041 gggtactgga cttcagcctc gtctccggag gcgtacagga tccgctcgac ccc
//
LOCUS       DAOSPL010000911         2092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_53188_length_2092_cov_2.775650, whole genome shotgun sequence.
ACCESSION   DAOSPL010000911 DAOSPL010000000
VERSION     DAOSPL010000911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53188_length_2092_cov_2.775650"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PLQ54_15115"
     CDS             <1..846
                     /locus_tag="PLQ54_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOS94649.1"
                     /translation="HVKEGAEPPPPENRRLGDALFPHQPNRPPDAPEQEQVEINAELT
                     WNDREVLRQADFDTMTADEWREAQRLLSQLKLVFEPLPTRRTRRATRPGSADWRATLQ
                     AMARHGGELWDTRWRAPRIQPAPLLVLADISGSMSRYSRMLLHFGHLLGHSDARVESF
                     VFGTRLTRTGPRDGVRKVAAGDFQGVGSDDVLESLADGFLGGVAAHSVHCRARVGDLE
                     VEIHRPDDVRRVFGHQAKRLFALLEGPFRGRAGHQASQPGRQARACAITPRHGAVVHV
                     HRILA"
     gene            1633..>2092
                     /locus_tag="PLQ54_15120"
     CDS             1633..>2092
                     /locus_tag="PLQ54_15120"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOS94650.1"
                     /translation="MPPKLVAFVGSPRPGGNTDTLADAALSAFAQAGGEAEKVILNSL
                     SIRPCQGCLACMRGDGLEDFCAVRDDMQGLHRKLLEADALLWATPVYMWSPSAQMKTF
                     LDRLFPFGDYQKTRWRAALAGKPVGLAIVYADPDPLTSGVVQTRDVLRAVV"
BASE COUNT          332 a          733 c          702 g          325 t
ORIGIN      
        1 cacgtgaagg agggcgccga gccgcctccg cccgagaacc gccgcctggg cgatgcactc
       61 ttcccgcacc agcccaaccg cccgcccgac gcgcccgagc aggagcaggt ggagatcaac
      121 gccgagctga cctggaacga ccgcgaggtg ctgcgccagg ccgacttcga caccatgacg
      181 gccgatgaat ggcgcgaggc ccagcgcctg ctgtcgcagc tgaagctggt gttcgagccc
      241 ctgcccacgc gccgcacccg ccgcgccacc cgccccggca gcgccgactg gcgcgccacg
      301 ctgcaggcca tggcccgcca cggcggcgag ctgtgggaca cgcgctggcg cgcaccgcgc
      361 atccagcccg cgccgctgct ggtgctggcc gacatctcgg gcagcatgag ccgctactcg
      421 cgcatgctgc tgcacttcgg tcacctgctg ggccacagcg acgcgcgggt ggagagcttc
      481 gtcttcggca cccggctcac ccgcacgggc ccgcgcgatg gcgtccgcaa agtggcggcg
      541 ggcgacttcc agggagtcgg gagcgacgac gtcctcgagt ccctggccga tggcttcctc
      601 ggcggtgtag ccgcgcactc ggtgcactgc cgggctcgcg taggtgatct tgaggtcgag
      661 atccatcgtc cagacgacgt ccgacgcgtg ttcggccacc aggcgaagcg tctgttcgct
      721 ctccttgagg gcccgttccg cggccgtgcg ggccaccaag cgagccaacc aggacgacag
      781 gcccgtgctt gcgccatcac accccgccat ggggccgtgg tccacgtaca tcggattctc
      841 gcatagaacc ccatcgagga ggatgaacgg gtaggcgcgg atctcctgtt cgagctcgtc
      901 atcagacacc gaggaacggt cgtagacgca ggccgaacgt acgggcacag cttccgggct
      961 gacggtgccg ggcgcgaggc ccaagcggcg cgccatatcc ctgtgtccgg cgtcccagac
     1021 tgcggtcaca cccacggcct cggtggcccc gagagcatgg atgacctcgt cgtcggtgcc
     1081 ggtgaacagc cggcccgtca tgcgcagtgg accggtgtcc catccggcct cctgaaggag
     1141 ccgcagaagg ctttgggacg cctcaggtcc ggccaagtac gcgcaccggt cgcctcggcc
     1201 gagtccggcc gcaatgaggg gcaggatgct gttcggagct tcctcggctg atgcgctgat
     1261 aacacagacg gagtcgcccg gacgcagtgt cgtcagagcc tcgggaacca atcccatcgc
     1321 agtccctatg tcccgtacgc ccaggcccat agcgctgcca ttggtccaca ctgaaggcac
     1381 cgaaaggcat tgcttcgtgc gtaggcatac catcccgcgc cgccatcggc tagcgctctt
     1441 cgtgctgccg cgtgcccttg acacgctgcc gtgtccataa cggtacgtgc aagcgttggt
     1501 tcacggtctg gttaccgaac gctcgggcat gccatacccc gtaccgacca gcttcgccgg
     1561 cctcgggaag gtgtgatggc gggccgggcg aaccgcgccg ccatgctgac accgggtgag
     1621 aagggggtcg ccatgccacc caagctggtt gccttcgtgg gaagccccag gccgggcggc
     1681 aacaccgaca cactggccga cgccgcgctc tcggcattcg cgcaggctgg aggcgaggcc
     1741 gagaaggtca tactgaactc cctcagcatt cggccctgtc aggggtgcct ggcctgcatg
     1801 cgcggggacg gcctcgagga cttctgcgcg gtgcgcgacg acatgcaggg cctgcaccga
     1861 aagttgcttg aggctgacgc gctcctgtgg gccacacccg tgtacatgtg gtcgccttcg
     1921 gcccagatga agacattcct ggatcgcttg ttcccgtttg gtgactacca gaagacgcgg
     1981 tggcgggccg ctctggccgg caagcccgtg gggctggcca tcgtctacgc cgatcccgac
     2041 ccactcactt cgggtgtggt gcagacgcgg gacgtgctgc gggccgtggt tg
//
LOCUS       DAOSPL010000912         2090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_53252_length_2090_cov_3.075184, whole genome shotgun sequence.
ACCESSION   DAOSPL010000912 DAOSPL010000000
VERSION     DAOSPL010000912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53252_length_2090_cov_3.075184"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2090)
                     /locus_tag="PLQ54_15125"
     CDS             complement(<1..>2090)
                     /locus_tag="PLQ54_15125"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOS94651.1"
                     /translation="ALAEYDYRMLLVGRAALWAASREGDVAFESVVAGFSPSIAGGTT
                     MASEVTLRSTAAEKVDATVELELCRASDGQRTPLGKAEVTLPPGESVRVPVKLPTLRA
                     GGYYVDAVAKSKRGTEAFAAGSFAFESPFGVGQVKLDRPFVEVGETIRGTATLRGPVP
                     DGAVLQLRYRDSYDRVLDQQDVALSPGQTEVPLSYQATTFATNWMRAEALLRAGDREI
                     EQKDASFLVPKRRHNQHNFVMWDAPMDVLGPFAWRQMQAVGYNVCLLGTFGPPAREAP
                     ASLLACDASIAPYSTRILDPKDENGYMQPVCWNDDPKAADYVQGIVDAQIHMREQGVF
                     VYSLGDEGVTLGCCVHPACIATYRRWLAEQYGTIEALNASWGTTYASFDQVDLLDHAD
                     NMEAAARTSCFPRWYDRQAFARWNLMHFVRRFVEGYRELDPEALTGFEGTGGFGDDYD
                     AILATNTFYGPYPSIGDDLVRSIAPRSTVRSNWMGYSKTGDALSDAAWRMVMKGMDSI
                     WFWMWSGIGDWRGYLRPTLDLWPATADLAEEMRPVRQGLGDLLIRSKVEHSGIAIYYS
                     VPSTLSAQLENGSDFVSPQTDHERWLQFIYELGLDARYVSTAMLRSGALTTDEFRVLL
                     LPMTQALSAEEAAAIRKFAEAGGTVIADVRPGIYDGHCKPLEPGALDDLFGIRRTGRG
                     KAADQPVSIDGTMA"
BASE COUNT          325 a          674 c          761 g          330 t
ORIGIN      
        1 cggccatggt gccatcgatg gacacgggct ggtcggccgc cttcccgcgg cccgtgcggc
       61 gtatgccgaa caggtcatcg agcgcgcccg gctcgagcgg cttgcagtgg ccatcgtaga
      121 tgcccggccg cacatcggcg atgaccgttc cgccggcctc ggcgaacttg cggatggccg
      181 cagcctcctc agcgctcagc gcctgagtca tcggcagcag tagcacgcgg aactcgtcgg
      241 tcgtgagcgc gccggacctc agcatggcgg tgctcacgta gcgggcatcg aggccgagct
      301 cgtagatgaa ctgcagccag cgctcgtggt cggtttgggg cgagacgaag tcggagccgt
      361 tctcgagctg ggctgatagc gttgagggca ccgagtagta gatggcgatg ccgctgtgct
      421 cgaccttcga cctgatgagc agatcgccga ggccctggcg gacggggcgc atttcctcgg
      481 ccaggtcggc ggtggcgggc cacagatcga gcgtgggtcg cagatagccg cgccagtcgc
      541 cgatgccgga ccacatccag aaccagatgc tatccatgcc cttcatgacc atgcgccagg
      601 cggcatcgga gagggcgtcg ccggtcttgc tgtagcccat ccagttcgac cgcaccgtgc
      661 tgcgcggggc gatggagcgg accaggtcgt cgccgatgct gggatagggg ccatagaagg
      721 tgttggtggc gaggatggcg tcgtagtcgt cgccgaagcc gccggtgccc tcgaagccgg
      781 tcagcgcctc ggggtcgagt tcgcggtagc cctcgacgaa ccggcgcacg aagtgcatga
      841 ggttccatcg ggcaaaggcc tgccggtcgt accagcgcgg gaagcaggag gttcgcgcgg
      901 cggcctccat gttgtcggcg tggtcgagga gatcgacctg gtcgaaggag gcgtacgtgg
      961 taccccacga ggcgttcagc gcctcgatgg ttccgtactg ctcggccagc caacggcggt
     1021 aggtggcgat gcaggcggga tggacgcagc acccgagggt gacgccctca tcgcccagcg
     1081 agtagacgaa tacgccctgc tcgcgcatgt gtatctgggc gtcgacgatg ccctgcacgt
     1141 agtcggccgc cttggggtcg tcgttccagc atacgggctg catgtagccg ttttcgtcct
     1201 tcggatcgag gatgcgagtg ctgtaggggg cgatggaggc atcgcaggcc aggagggacg
     1261 ctggcgcctc gcgtgccggc gggccgaagg tgccgagcag gcacacgttg tacccgacgg
     1321 cctgcatctg ccgccaggcg aagggcccga gcacatccat gggcgcatcc cacatgacga
     1381 agttgtgctg gttgtgccgg cgtttgggga cgaggaacga ggcgtccttc tgctcgatct
     1441 cgcggtcgcc ggctcgcagc agtgcctcgg cgcgcatcca gttggttgcg aacgtggtgg
     1501 cctggtagct caggggcacc tcggtctggc ccggcgacag ggccacgtcc tgctggtcga
     1561 ggacgcggtc gtagctgtcg cgatagcgca gttggagcac ggcgccatcg ggcaccggcc
     1621 cacgcagcgt ggccgtgccg cggatggtct cacccacctc gacgaagggg cgatcgagct
     1681 tcacctggcc gacgccgaag ggactctcga aggcgaacga cccggcagcg aaggcctcgg
     1741 tcccgcgctt cgacttggcg accgcatcca cgtagtagcc ccccgcccgg agggtcggca
     1801 gcttcacggg cacgcggacc gactccccgg gcggcagcgt gacctcggcc ttgcccagtg
     1861 gcgtccgctg gccatccgaa gcgcggcaga gctcgagctc gaccgtggcg tcgaccttct
     1921 ccgccgccgt gctgcggagg gttacctccg aagccatcgt ggtcccgccg gcgatcgagg
     1981 ggctaaagcc ggcgacgacg gactcgaacg ccacatcgcc ctcgcgggag gccgcccaca
     2041 gggccgcccg gccgaccagg agcatgcggt agtcgtactc ggccagggcg
//
LOCUS       DAOSPL010000913         2088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_53310_length_2088_cov_2.917855, whole genome shotgun sequence.
ACCESSION   DAOSPL010000913 DAOSPL010000000
VERSION     DAOSPL010000913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53310_length_2088_cov_2.917855"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1717)
                     /locus_tag="PLQ54_15130"
     CDS             complement(<1..1717)
                     /locus_tag="PLQ54_15130"
                     /inference="COORDINATES: protein motif:HMM:NF026221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOS94652.1"
                     /translation="MDDRLVTLCGLPMAMLAAGAAGLGTPWYQQALVGMEVGPTGAQF
                     GAEGGDPLYAARFNGSEIVRRCREAGSEYVVVWARDGVYAYYDSAVQPKAPGLRERDV
                     LREAVREGRRLGMPIIAYCVLQYPTTALAEHPEWAMRDADGKPIPDRVCFNSPYREHV
                     KDLLKEMLAYGIDGFHLDMVDQGFGPPHGCWCDHCARLYEARYGAAMPTGVTWDESWN
                     RMLELRYETSAGFERELTQFIRSEAPGVTVDYNYHGNPPFSWEVGQRPVQHAVNGDFV
                     TGETGIWGFSALTTGLNVEIYRAATPGRPVQVAMQRGVRMYHDQTTRPLADMQWELYT
                     LLAHGAFVTMVDKTAYDGWLDPVAYERIGELFAEARRRRDEFGHAPVADVALWFSDRS
                     RDWYGRQDPSRYFAGFLGAHKAMTYEHLPWSVVLEENASLATLKQTPVVLLPNAAIVG
                     EATVRLLSDYVTGGGNLIVTGISGCFDHMGQPRPHSALENLIGGRLVRPLESRDNHVR
                     FARGQGPEALTAGLRPDWPFLVEGPAVVYEATEATAIGELLAPHRTVRQIQGLEATDW
                     PMSAGE"
BASE COUNT          304 a          703 c          734 g          347 t
ORIGIN      
        1 tttcgccggc gctcatgggc cagtcggtcg cctccaggcc ctggatctga cggacggtgc
       61 ggtgcggtgc gagcaactcg ccgatggcgg tcgcctcggt cgcctcgtac accactgcgg
      121 ggccttcgac gaggaatggc caatcagggc gcaggcccgc cgtgagtgcc tccggaccct
      181 gtccccgggc aaaccggacg tggttgtctc gggactcgag gggccgcacc aagcgcccgc
      241 cgatgaggtt ctcgagggcc gagtgggggc gcggttgtcc catgtggtcg aagcagccgg
      301 agatgcccgt gacgatgagg ttgccgccgc cagtcacgta gtcgctgaga aggcggacgg
      361 tggcctcgcc gacgatggcc gcgttgggta gcagcaccac cggggtctgc ttcagggtcg
      421 cgagggacgc gttctcctcg aggacgacgg accatggcag gtgctcgtag gtcatggcct
      481 tgtgcgcgcc gaggaagccg gcgaagtacc ggctcggatc ctggcgcccg taccagtcgc
      541 ggctcctgtc gctgaaccag agtgctacgt cagcgacggg cgcatgacca aactcgtcgc
      601 ggcggcggcg cgcctcagcg aacagctcgc cgatccgctc gtacgcgacc gggtcgagcc
      661 atccatcgta cgccgtcttg tcgaccatgg tcacgaaggc gccgtgggcc aggagcgtgt
      721 acaactccca ctgcatgtcg gcaagcggtc gggtcgtctg gtcgtggtac atcctcacgc
      781 cgcgctgcat cgccacctgt acgggtctgc cgggggtggc cgcccggtag atctcgacat
      841 tgaggcccgt ggtgagggcg ctgaagcccc agatgcccgt ctcgcccgtc acgaagtcgc
      901 cgttcacggc gtgctggacc ggtctctggc ccacctccca tgagaaggga gggttgccgt
      961 ggtagttgta gtcgacggtg acgcccgggg cctcggagcg gatgaactgc gtgagctcgc
     1021 gctcgaagcc ggcgctcgtc tcataccgga gttcgagcat ccggttccag ctctcgtccc
     1081 acgtgacgcc ggtgggcatg gccgcaccat agcgggcctc gtagagccgg gcgcagtggt
     1141 cgcaccagca tccgtggggc gggccgaagc cctggtcgac catgtcgagg tggaacccgt
     1201 cgatcccata ggcgagcatc tctttgagca ggtctttgac gtgctcccgg taggggctgt
     1261 tgaagcacac ccggtctggg atgggtttgc catcggcgtc gcgcatggcc cactccgggt
     1321 gctccgcaag agccgtcgtg gggtactgga gcacgcagta agcgatgatg ggcataccga
     1381 ggcggcgccc ttcccgcacc gcctcacgca gaacgtcgcg ctctctgagg ccaggcgctt
     1441 tgggctggac ggccgagtcg tagtatgcat agactccatc gcgggcccag actaccacgt
     1501 actcgcttcc cgcctcgcgg catcgccgga cgatctcgct gccgttgaac cgcgccgcat
     1561 agagcgggtc gccgccctcg gcgccgaact gggcgccggt ggggccgacc tccatcccga
     1621 cgagcgcctg ctgataccag ggcgtcccca gaccggccgc gccggccgcg agcatcgcca
     1681 tgggcaggcc acacagcgtc acaaggcggt catccattcc gtggactcca ttgccgcgcg
     1741 cccgatcagc cagacgcgct tcgcgatccc ccctgcgcgc cgctagccgt cagtgcccgg
     1801 ggggccggga ggcatgacgg tcacatcggg ctccatcggc ccgtggacgc ggtcgtggag
     1861 gggcggtgcg gcgtacgggc cttcggcgca ccacctgggc ccaagttacc gccaatggcg
     1921 ccctgtcccc tcctctcttg cggatctgcg ttgcttgcgt ccacgcccta ctggcccact
     1981 tcgatgcggg ccgttgccgt ccggtccgta ttggcgggca aggtgacggc ctggccgtcg
     2041 agcagggcga agaagaggtg gatggtgctc gggccgccgt cgagcagg
//
LOCUS       DAOSPL010000914         2084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_53438_length_2084_cov_3.517496, whole genome shotgun sequence.
ACCESSION   DAOSPL010000914 DAOSPL010000000
VERSION     DAOSPL010000914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53438_length_2084_cov_3.517496"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="PLQ54_15135"
     CDS             complement(<1..1076)
                     /locus_tag="PLQ54_15135"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOS94653.1"
                     /translation="MPDEDAAGAPDRHGVAVSDEPKPDPAEPEAEPGVKVMPILVADQ
                     VVLFPHMIAPLIVSRESDAAAMDETLVRVKQIGVVATRRDPGEETPQPQDLYEFGCTA
                     QILKMLKVPDGTRRAIIQGISRMRIERIVQTDPFIMAEVRTLEPIEASDVEVDALVRT
                     VRETFRTIVELSSDIPDEAAVQAANIPEPGKLADFIVSNVHLDQSQRQEILEALDVKQ
                     RLSRLHSLIVGELQVLELGQKIQTDARGEMERAQREYFLRQQLEAIRKELGDEDDQEA
                     AIRELREAIEQAEMPPAAREQAEKELRRLGRIPSASAEYTVSRTYLDWLVQVPWSKST
                     VDVLDVTQAAAILNEDHYGLERVK"
     gene            complement(1172..1627)
                     /locus_tag="PLQ54_15140"
     CDS             complement(1172..1627)
                     /locus_tag="PLQ54_15140"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOS94654.1"
                     /translation="MFALPRRSPDEDLIPARPRRSSPFDELLRSAGHVLFVRDMVWVP
                     PVDVYETEREIIVKVELAGVTVADLEVTIDKDVLSIAGQRPDPSGLANDVVQCVHHRE
                     IDCGRFERTIRLPARVDRQTIRAHLSDGYLDVVLQKETGRPAPYSVKIG"
     gene            complement(1654..>2084)
                     /locus_tag="PLQ54_15145"
     CDS             complement(1654..>2084)
                     /locus_tag="PLQ54_15145"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOS94655.1"
                     /translation="GLKVLDELLEEFDVRIAIHNHGPGNPFDKITDVTKAMEGHHERI
                     GACVDTGWYIASGEDPIEAIQTFGERVYAVHVKDMTAERSFTEVGKGELDVRAMFDVL
                     GTLHYEGILALEYEEHPDDPVPYVDECLAAMREAIRPAAE"
BASE COUNT          322 a          725 c          653 g          384 t
ORIGIN      
        1 tccttcacgc gctccaggcc gtagtggtcc tcgttgagga ttgccgcggc ctgggtcacg
       61 tccagcacat ccaccgtgct cttggaccag gggacctgga cgagccagtc gagataggtc
      121 cgcgagacgg tgtactcggc cgatgcgctc gggatgcgcc ccagccggcg gagctccttc
      181 tcggcctgct cccgggccgc cggcggcatc tcggcttgct cgatcgcctc ccggagctcc
      241 cggatcgccg cctcctggtc gtcctcgtca cccagctcct tgcgaatggc ctccagctgc
      301 tgccggagaa agtactcccg ctgagctctc tccatctcgc cgcgggcgtc ggtctggatc
      361 ttctgcccca gctccagaac ctgcagttcg cccacgatca ggctgtggag ccggctgagc
      421 ctctgcttca catccagagc ctcgaggatc tcctggcgct gggactggtc gaggtggaca
      481 ttcgagacga tgaagtcagc cagcttgccc ggttccggga tgttcgccgc ttgaaccgcg
      541 gcctcgtcgg ggatatcgga cgacaactcg acgatggtgc ggaacgtctc ccggaccgtg
      601 cggaccaagg cgtccacttc gacgtcgctc gcttcgatcg gctccagcgt gcgtacctcg
      661 gccatgatga aggggtcggt ctgcacgatc cgctcgatgc gcatccgaga gatgccctgg
      721 atgatggccc tgcgtgtccc gtcgggcacc ttcagcatct tcaggatctg cgcggtgcag
      781 ccgaactcgt aaagatcctg cggctggggc gtctcttcgc ccgggtcgcg gcgggtggcc
      841 accacgccaa tctgcttcac gcgcacgagc gtctcgtcca tggcggccgc atccgactcg
      901 cgcgacacga tcagtggcgc gatcatgtgg ggaaacagca cgacctggtc ggccaccaga
      961 atgggcatga ccttgacgcc gggctcggcc tctggctcgg ccggatccgg cttgggttcg
     1021 tcgctgacag caaccccgtg gcgatccggg gcgcctgcgg cgtcctcatc gggcatctca
     1081 gtaccgtcct cctgggcagg ttgctgaccc ggcggcgcga ggccatatcc ggccgggccg
     1141 ggtggccgcg atcgcgttgc ccgccgggta cctagccgat cttgacgcta tagggcgcgg
     1201 ggcggccggt ctccttctga agcacgacgt ccaggtagcc gtcactgagg tgagcgcgaa
     1261 tggtctgccg atccacgcgc gcgggcaagc gaatcgtgcg ctcgaaccgg ccgcagtcga
     1321 tctcgcgatg gtggacgcac tggactacgt cgtttgccag ccccgaaggg tcggggcgtt
     1381 gccccgcaat cgagaggaca tccttgtcga tagtcacttc cagatcagcc acggtcaccc
     1441 cggccagctc gaccttgacg atgatctccc tctccgtctc gtacacgtcc accggaggga
     1501 cccagaccat gtcccgaacg aagaggacat gaccggccga gcgcagcagc tcatcaaaag
     1561 gcgacgaccg cctcgggcgg gccgggatca ggtcttcgtc ggggctcctg cgcggcagtg
     1621 cgaacatgcc ggccacctcc ctgtatcgac agcctattcc gctgccggcc gtatcgcctc
     1681 gcgcatggct gccaggcact cgtccacata gggcaccggg tcatccgggt gctcctcgta
     1741 ctcgagcgcg aggattccct catagtggag cgttcccagg acgtcgaaca tggcacgcac
     1801 gtccagctcg cccttcccca cctcggtgaa ggaccgctcg gccgtcatgt ccttgacatg
     1861 caccgcatag acgcgctcac caaacgtctg gatggcctca atgggatcct ctcccgacgc
     1921 gatgtaccaa cccgtgtcga cacaggcccc gattcgctcg tggtggccct ccatggcctt
     1981 ggtcacgtcc gtgatcttgt caaaggggtt ccccgggccg tggttgtgga tggcgatccg
     2041 cacatcgaac tcctcgagga gctcgtcgag caccttcagc cccg
//
LOCUS       DAOSPL010000915         2083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_53496_length_2083_cov_2.062130, whole genome shotgun sequence.
ACCESSION   DAOSPL010000915 DAOSPL010000000
VERSION     DAOSPL010000915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53496_length_2083_cov_2.062130"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..156
                     /locus_tag="PLQ54_15150"
     CDS             52..156
                     /locus_tag="PLQ54_15150"
                     /inference="COORDINATES: protein motif:HMM:NF018531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HOS94656.1"
                     /translation="MGYREGQPCPECGTAIEKIKTGSTSSFICPRCQV"
     gene            complement(253..849)
                     /locus_tag="PLQ54_15155"
     CDS             complement(253..849)
                     /locus_tag="PLQ54_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94657.1"
                     /translation="MNARLPAAAERREGARALTAGTLADDVWAAGLRGDLDGQHDPED
                     VWLDPLFPFGNWTLGTRDPATDRVLQAPIDLAPAGSVPTRAARAARPTELCSGGVDPH
                     RRRCGRDCSREGRNGHIGRHRHRLQAGGSPPAHSRPGVGQSSEKRRYGTPGWRPSPAR
                     GSWTVSTTEELRQAFEAAEDAGHIVKDNLVDTQSWEPK"
     gene            complement(1010..>2083)
                     /locus_tag="PLQ54_15160"
     CDS             complement(1010..>2083)
                     /locus_tag="PLQ54_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94658.1"
                     /translation="ESPLLSSFYKDQRDRGLTRVPYRSGGSPELALDWGREEVRDHYM
                     RLVREVLSFRGIEGLELDWMRFGWHFRIGREAEGGELLTDWIRRVRRLCDAAAQREGH
                     PVRLGCRVPSTPQTARMLGMDGAAWAREGLVDLVVPTPFWETCEFDMPIATWKGLLEG
                     TNCALAGGLEIRYQPCRGGWSSMMTPELAAGAATAVLAGGADYVYLFNYFAKGHLDNL
                     WSPESYVRTLSAMRGLATLNKLPRRHGVTFRDIVAPGEPSGRLLPAEPGLSIFRIQTG
                     PKPVGRSVQVLLELEAADGAEPEAPGVRVNSVECPAPTREGKAVLLYPVPDAALSDSE
                     TVIEATSAARIIRVEVVIGAPEA"
BASE COUNT          320 a          735 c          710 g          318 t
ORIGIN      
        1 gcctatgaga aggacctgta cggcaagccc ggcggcttct ccgagttcct cgtgggctac
       61 cgcgagggcc agccgtgtcc cgagtgcggc acggccatcg agaagatcaa gaccggcagc
      121 acgtcgtcgt tcatctgccc caggtgccag gtgtaggggg cgcccagagc atctcaggcg
      181 gggtctgcta tccactaaca ggagttcact ttccagcggc gactcagatg ggccgttgcc
      241 gcgatctctg cttcacttcg gctcccacga ctgggtgtcg acgaggttgt ccttgacgat
      301 gtgcccggca tcctcggcgg cctcgaacgc ctgccggagc tcttcggtgg tcgacacggt
      361 ccaggagccg cgcgcgggac tgggccgcca cccgggcgtg ccgtaccggc gcttctcgct
      421 gctctggccg acaccaggcc gactgtgcgc gggcgggctg cctccagctt ggagtcgatg
      481 ccggtgccga ccgatgtgac cgttccggcc ctcgcgcgag caatcgcggc cgcagcgccg
      541 cctgtgcggg tctacgccgc ccgagcacag ttccgtgggc cgtgcggccc tggcggcacg
      601 ggttggcaca gaccccgcgg gtgccaggtc tatgggcgcc tgcaagaccc ggtcggtggc
      661 tggatcgcgc gtacccagtg tccagttccc aaaggggaac agcgggtcca gccagacatc
      721 ctcggggtcg tgctgaccat ccaagtcgcc gcgcaggccg gctgcccaca catcatcggc
      781 gagcgtgccc gcagtcagcg cccgggcgcc ctcgcggcgc tccgcagcag cgggtaggcg
      841 tgcgttcata ggcactcgca gtcacccgtg tggtgatagc gccaccatgc ctgtgcggtc
      901 agctcggcac ccgaggagca cacgtggctg atccatacgt atcgtctgcc cgccgtctca
      961 ccgggctcga cgaggagccc aaggctgccc tcgaactggg ctcgctcgcc tacgcctcgg
     1021 gcgccccgat gaccacctcg acgcggatga tccgcgcggc cgaggtcgcc tcgatcaccg
     1081 tctcgctgtc cgacagcgcc gcatcgggca ccgggtagag cagaacggcc ttgccctcgc
     1141 gcgtgggcgc ggggcactcg acgctgttca cacggacgcc aggcgcttcg ggctcggcac
     1201 cgtcggcggc ctcgagctcg agcagtacct gaaccgagcg gccaacgggc ttggggccgg
     1261 tctggatgcg aaagatcgac aggccgggct ccgcgggcaa cagccgccca gagggctcgc
     1321 ccggcgcgac gatgtcgcgg aaggtcaccc cgtgccggcg gggtagcttg ttgagggtgg
     1381 cgagaccgcg catggcggag agggtacgga cgtagctctc ggggctccac aggttatcga
     1441 ggtggccctt ggcgaagtag ttgaacaggt agacgtaatc ggcgccgccg gccagaacgg
     1501 cagtggcggc cccggcggca agctcgggcg tcatcatgct ggaccagccg ccgcggcagg
     1561 gctggtagcg gatctcgagc ccaccggcca gcgcgcagtt ggtgccttca agcagtcctt
     1621 tccaggtcgc gatgggcata tcgaactcgc aggtctccca gaacggcgtt gggacgacca
     1681 ggtccaccag accctccctc gcccacgcgg caccgtccat tccgagcatg cgggcggtct
     1741 ggggcgtcga cggcacgcgg caaccgagac gcaccgggtg cccctcgcgc tgggccgccg
     1801 catcgcacag ccgcctgacc cggcggatcc agtcagtcag cagctctccg ccttcggcct
     1861 cccggccgat gcggaagtgc cagccgaagc gcatccagtc gagctcgagg ccctcgatac
     1921 cccgaaatga cagcacttct cgaacaagcc gcatgtagtg gtcgcggacc tcttcgcgcc
     1981 cccaatccag ggccagctca ggcgacccgc ccgagcggta tggcacgcgg gtcagcccac
     2041 ggtccctctg gtccttgtag aaactgctca gcagcgggct ctc
//
LOCUS       DAOSPL010000916         2079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_53632_length_2079_cov_2.376976, whole genome shotgun sequence.
ACCESSION   DAOSPL010000916 DAOSPL010000000
VERSION     DAOSPL010000916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53632_length_2079_cov_2.376976"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLQ54_15165"
     CDS             complement(<1..397)
                     /locus_tag="PLQ54_15165"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOS94659.1"
                     /translation="MNPWIMAGPAALAGAWFGSAALTHAVVTRGNRGVVHRGRHPSAV
                     ALTFDDGPNPPHTQRICDILEAHGAKGTFFVLGRQVDRYPDTLGATVERGHEVQLHGH
                     NHLHAWFTPPGLHQADLRRGLEAIERASGT"
     gene            complement(394..1671)
                     /locus_tag="PLQ54_15170"
     CDS             complement(394..1671)
                     /locus_tag="PLQ54_15170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS94660.1"
                     /translation="MASGERGIPWRQFLPAVAVMSLAEFAHGGLLYVLLREHGERVLR
                     LSPAEIGVTISFYTLADLSMKVLSGALADRLGRRAMLVFAMSGSLATLAGMPFATGVW
                     ILRALVFLHGVSASPVGPTVLALTADRVDPERRSTAVGVVFTAWFAAIGCGFLVLMLL
                     SRLDTRLLFGLLGVEFAVALLIAATLVPRGGRGAVEGRAARRPLLEVGLDAARRLTRK
                     RLLVVGLFCQTLTVGLLAPVLAPYLKGHQGLGEDQILILAIAGGGLAAGLIIPMGRMA
                     DRVSKQWLMVSSLAASGLVLAGLAYTRSFPVLIGLVGVAGCAYALILPTWNALLLLHA
                     HREQRALMLSAFMACEQLGFMAGPASSGFIEGRWGPRGPFLAASGVLLVIAAVYSFAA
                     PMFRGDPEALDPVPGSEEPEAAVCSVQSEEAMR"
     gene            complement(1671..1958)
                     /locus_tag="PLQ54_15175"
     CDS             complement(1671..1958)
                     /locus_tag="PLQ54_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94661.1"
                     /translation="MVTKAGGITVSEALARGLPLLLHRPLPGHEEWNVRYVTEGGAGL
                     LSWGARELAANLSGLLSAPDRLSAMSERARALGQPEAARRVAEALVGLCGV"
BASE COUNT          394 a          815 c          618 g          252 t
ORIGIN      
        1 gtgtgcccga ggccctctcg atcgcctcca gcccgcgccg gaggtcagcc tggtgcaacc
       61 cgggcggtgt gaaccaggca tgcaggtggt tgtgcccgtg gagctgcacc tcatgccccc
      121 gctccaccgt ggcccccaga gtgtccgggt aacggtccac ctggcgtccg agcacaaaga
      181 acgtcccctt cgcgccatgc gcctcgagga tgtcgcatat ccgctgtgtg tggggcgggt
      241 tcgggccatc gtcgaaagtc agagcgacgg ccgagggatg ccgtccacga tggaccacgc
      301 cgcggttgcc ccgggtcacc accgcatggg tgagagccgc cgagccaaac caggcgcccg
      361 ccagggccgc tgggccggcc ataatccacg ggttcatcgc atcgcctcct cggactgcac
      421 cgaacaaacc gccgcttctg gctcctcgga acccggaacc ggatcgagcg cctccggatc
      481 accgcggaac atcggcgccg cgaatgagta cacagccgcg atcaccagca ggacacccga
      541 tgccgccagg aacgggccac gaggccccca acggccctca atgaagcccg acgaagccgg
      601 gccggccatg aaacccagct gctcgcatgc catgaacgcc gacagcatca gcgcccgttg
      661 ctcccggtgc gcgtgcagca gcagcaatgc gttccatgtc ggcaggatca gcgcgtaggc
      721 gcacccggcc acgccgacca agccgatcag cacggggaac gagcgcgtgt aggcgagccc
      781 cgccagcacc aggcccgacg ccgcgagact gctcaccatc agccactgct tgctaacccg
      841 atccgccatg cgtcccatcg ggatgatcag gccagccgcc agtcccccgc ccgcgatggc
      901 caggatcaga atctgatcct cccccaggcc ctggtggccc ttcagatacg gcgcaagcac
      961 cggcgccagc aggccgaccg tgagcgtctg gcagaacaac cccaccacca gcagcctctt
     1021 tcgagtgaga cgtcgagccg cgtccaggcc tacctcgagc agcggcctcc gcgcggcccg
     1081 cccctcaacc gcaccccgcc caccacgtgg caccagcgtg gccgcaatca gcaacgcgac
     1141 ggcaaactcc acgcccagga gcccaaacag cagacgcgta tcgagccggc tcagcagcat
     1201 gagcaccagg aagccgcacc cgatcgccgc aaaccatgcc gtgaagacca cccccacggc
     1261 cgtgctccgc cgctccggat ccacccggtc tgccgtcagc gccagaacgg tcggccccac
     1321 cggcgacgcc gaaaccccgt gcaggaacac cagcgcccgc agtatccaca cgcccgtcgc
     1381 gaacggcatg ccggcaaggg tggcaagcga gccggacatg gcaaacacta gcatggcccg
     1441 ccggcccaag cggtcagcca gggctcccga aagcactttc atcgatagat cggccagcgt
     1501 gtaaaacgag atggtgacgc cgatctcggc cggcgagaga cgcagaaccc gctcgccatg
     1561 ctcgcgaagc agtacgtaca gcagaccgcc gtgcgcgaac tccgccagtg acatcacggc
     1621 cacggcaggc aggaactgcc gccaggggat accccgctcg cccgatgcca tcagactccg
     1681 cacaacccaa ccagcgcctc agccacgcgt cgagcggcct ccggctgtcc cagcgcgcgt
     1741 gcgcgctccg acatggcgct caggcgatcc ggtgccgaca gcagcccgct caggtttgcg
     1801 gccagctccc gagcccccca agacaacaga ccagcgcccc cctcggtcac gtaccgcacg
     1861 ttccactcct catgcccggg cagcggccga tgcagcaaca gcggcaggcc gcgggccaac
     1921 gcctcgctca ccgtgatccc gcccgccttc gtcaccatct cctccgttgc tgcgctcggg
     1981 gcggccgggc tgctatgcga ccatgcagcg ctgccggccc cgtgcgctca agaaatcgtg
     2041 gcctgacccc gggcgaaggc tcgcttgttc agctccctc
//
LOCUS       DAOSPL010000917         2075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_53768_length_2075_cov_3.294059, whole genome shotgun sequence.
ACCESSION   DAOSPL010000917 DAOSPL010000000
VERSION     DAOSPL010000917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53768_length_2075_cov_3.294059"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLQ54_15180"
     CDS             complement(<1..439)
                     /locus_tag="PLQ54_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94662.1"
                     /translation="MRAFIAAVLLLGGAAGSCLGAVREAWPPFWPEWSKRDVAAEACQ
                     GDIAAALAWISGWSGVPCIVPVLNGEADGAPGWLRRPAERHLPCDTYRAVHNWARANE
                     HEGFATPHCVVMAPLAGRPWGHGPFSSPEGVEAGPGGRLFAGSL"
     gene            complement(470..1006)
                     /locus_tag="PLQ54_15185"
     CDS             complement(470..1006)
                     /locus_tag="PLQ54_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94663.1"
                     /translation="MVQRLVVLVPVLALVLAGSGWASSCYEVDGESSSMPAYTQTLTC
                     DTEYIPCNRYLELPRWVYLDCAGHAEWQYPGAFNCVVDELSPCFYDESLEASDFQVLE
                     AVDEVVDLSYWATARFKVVLQHKRTRGVVATQMHYRGMPMFGGSGFCVTDECQSYSGI
                     RWNWEMDPLNNITLQDCN"
     gene            complement(1006..>2075)
                     /locus_tag="PLQ54_15190"
     CDS             complement(1006..>2075)
                     /locus_tag="PLQ54_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94664.1"
                     /translation="LSGEWVQPLSWDCPFGRAVARVVRRLDEPAREGVNGDPPPAVLL
                     TGRATCSWRPAGGGRGTVFGGLIGREHGIPPKTGLLVSLSPDPGGPRSLATAKWRERV
                     ARPATPPSAPPEPWRLADLIPPLVDLCAVLGGARVAYVSPAPVGPQEPLRACSAEGIA
                     VTPSAAFETTEMLVAIRNRPVREGLDALCMSLGSEAKPVGGGVFVGPGGQSRPDWAVG
                     SIGELLDRIVRPSFRAALAGTGADPEWADEAWRCVAWRPDTALSEAQRNMFAGAPGLL
                     GLFGPESPPPAGSERAVRIGFDLRLGRPVEEWTLTGPAGTYAVLGHENLSAPGVGLFE
                     PRGDAATPRDSVADTREEGAR"
BASE COUNT          343 a          771 c          664 g          297 t
ORIGIN      
        1 ccagcgaccc ggcgaagagc cggccaccgg gaccggcctc gacgccctcg gggcttgaga
       61 aggggccatg gccccacggg cgacccgcta gcggcgccat taccacgcaa tgcggcgtgg
      121 cgaacccctc atgctcattc gcccgcgccc agttgtgcac cgcccggtat gtgtcgcaag
      181 ggaggtggcg ctccgcgggc cgcctgagcc agccaggcgc cccgtcagct tccccgttga
      241 gcaccggcac gatgcagggc acccccgacc atccggagat ccacgcgaga gcggcagcaa
      301 tgtcgccctg gcaggcctct gccgccacgt cccgcttgga ccactcgggc cagaagggcg
      361 gccaggcctc gcgcaccgcc cctaggcagc tgccggcggc gcctccaagc aggaggaccg
      421 cagcgatgaa cgcgcgcatg gctagccccc ctcgtggatc ccgcccggcc tagttgcagt
      481 cctggagagt gatgttgttg agcggatcca tctcccagtt ccagcgtatg cctgagtagg
      541 actggcactc gtctgtcaca cagaacccac tgcccccgaa catcggcatc cctctgtagt
      601 gcatctgtgt cgcgacgacc ccacgcgtcc tcttgtgctg caggacgacc ttgaaccgtg
      661 ccgtagccca gtacgacagg tcaacgacct cgtcgaccgc ttcgagcacc tggaaatcgc
      721 tggcttcgag ggactcgtca tagaagcacg gcgagagttc gtcaaccacg cagttgaacg
      781 cgcccgggta ctgccattcc gcgtggcccg cgcagtcgag gtacacccac ctcgggagct
      841 ccagataccg gttgcagggg atgtactccg tgtcacacgt gagcgtctga gtgtaggcgg
      901 gcatcgagga gctctcgccg tccacctcgt agcagctcga ggcccacccc gacccggcga
      961 gcactagcgc aagcaccgga acgagcacga cgagtctttg caccatcacc ttgcaccttc
     1021 ctctcgtgtg tctgcgacgc tgtcccgtgg cgtcgcagcg tccccgcggg gctcaaagag
     1081 ccccacgcca ggcgccgaca ggttctcatg gcccagcacg gcgtaggtgc cggccgggcc
     1141 cgtcagggtc cactcctcca caggccggcc cagcctcagg tcgaagccta tccgcactgc
     1201 cctctcgctt ccggctggag gcggggactc tgggccgaag agccccagaa gccccggcgc
     1261 tcccgcgaac atgttccgct gcgcctcgga cagggccgtg tccggccgcc atgcgacgca
     1321 ccgccacgcc tcatcagccc actctgggtc agcgcctgtg ccagccaggg ctgcccgaaa
     1381 cgacgggcgg acgatacggt ccagcagctc accgatggag ccgacagccc agtctggccg
     1441 gctctggccg cccggaccca cgaacacgcc gccacccacc ggtttggcct cactcccaag
     1501 cgacatgcac aaggcatcca ggccctccct gacaggccgg ttgcggatgg cgaccagcat
     1561 ctccgttgtc tcaaatgccg cggacggcgt gacggctatg ccctcggcag agcaagcccg
     1621 caggggctcc tggggcccaa cgggcgcagg cgagacgtag gccactcgcg cgcctccgag
     1681 cactgcgcac aggtcgacga ggggcggtat caggtctgcg agtcgccatg gctcgggagg
     1741 tgcggatgga ggagttgcgg gccgagcgac cctttcgcgc catttggcgg ttgccagcga
     1801 gcgcggccct cctggatccg gcgagagaga gacgagcaac ccggtctttg gggggatgcc
     1861 gtgctcgcgg ccgatcagcc ctccgaagac cgtcccgcgt cccccgccgg cgggcctcca
     1921 ggagcacgtg gccctgcccg tcagcagcac ggcagggggc gggtcgccgt tcactccctc
     1981 tcgcgcgggc tcgtccagtc tacgcaccac acgcgcgaca gcgcgcccga aggggcagtc
     2041 ccacgagagt ggctgcaccc actcgccgct cagga
//
LOCUS       DAOSPL010000918         2075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_53771_length_2075_cov_3.187624, whole genome shotgun sequence.
ACCESSION   DAOSPL010000918 DAOSPL010000000
VERSION     DAOSPL010000918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53771_length_2075_cov_3.187624"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1770
                     /locus_tag="PLQ54_15195"
     CDS             <1..1770
                     /locus_tag="PLQ54_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94665.1"
                     /translation="AVAGEVRVWTLVPRSVRTATVRAVSWSGEEEASVACRVHWRGFT
                     GIGRFELPALSRGFHRLSASTDRDDPPDHRYSALAVLAPGEARIRSVFDTLTPVAQCP
                     GLQGIDLEYVDQAALPFEIQDLGANFLQFHLNPKQLDNGEFDELIALCQALGTRFALN
                     NEWCNWAPESKAPDGTDRFVAPGGCHRWDLEEPALRTAAATGLFEGVVYDEGEHMQLC
                     RNAYAALPDTDHRKPYIVETTGMTLLEAYHSFTAATRQLVERNRALGARTIVESVFPA
                     LWEPMARAGVTLCPKLLKEDIHPVVLATALGAAKQYGADLWFSPDLWYTDRFPGHSVE
                     SYRAALELAYQAGVDHVYSEQIVAMCRVRGEVYDVTGHGRALREFIDRLRERPERPYA
                     YRDYAPEVAIIHFPDSDWGQASCYYWDTLYGAENLPPTAETAEWMQVWSLLTEGASDP
                     RAVNTNSSVYPMGSWRFTMPCMPVAVYGHRVGDGPLEGVKTLFLAGLDLPRPTLEAVG
                     RRVRAGAVCFAPRRQCPEGVAARAAALPMRVPDGRGSWIVVDGFAPEQLGEYLRLVPT
                     THEGMHLRFGEHAVRIGSVADEP"
     gene            1808..>2075
                     /locus_tag="PLQ54_15200"
     CDS             1808..>2075
                     /locus_tag="PLQ54_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94666.1"
                     /translation="MDVSTLTRLVVLSGLACAAGGQGLSSPEVVLRDWYFRESLVAIR
                     GWEVLLAEWEQQTPEDQRIPTLPVPDGAIVEWANPIPLTLVPPED"
BASE COUNT          309 a          684 c          755 g          327 t
ORIGIN      
        1 gcggtcgcag gcgaggtccg tgtgtggacg ctggtgcccc ggagcgttcg taccgctacg
       61 gtgcgcgccg tgagctggtc gggtgaggag gaggccagcg tcgcgtgccg ggtgcactgg
      121 cgcggcttca ctggtatcgg gcgattcgag ctgccggcgc tttcgcgggg gttccatcgg
      181 ctgtcggcgt cgaccgatcg ggacgaccca ccagatcacc gctactcagc cctcgccgtc
      241 ctggcccccg gggaggctcg tatccgcagc gtcttcgaca cgctgacgcc cgtagcgcag
      301 tgccccgggc tgcagggcat cgacctcgag tacgttgacc aggccgcact gccgttcgag
      361 atccaggatc tcggcgccaa cttcctccag ttccacctca acccgaagca gctcgacaac
      421 ggcgagttcg atgagctgat cgcgctctgc caggcgctcg ggacgcggtt cgcgctgaac
      481 aacgagtggt gcaactgggc gcccgagtcg aaggcgccag atggcaccga ccggttcgtc
      541 gccccgggtg gatgccaccg gtgggacctc gaggagcccg cgctacgcac ggctgcggcc
      601 accgggctgt tcgagggcgt cgtctacgat gagggcgagc acatgcagct ctgccgcaac
      661 gcctacgcgg cgctgccgga caccgaccac cgcaagccct acatcgtcga gactaccgga
      721 atgacgctcc tcgaggccta tcactcgttc acggccgcca cccgccaact ggtggagcgg
      781 aaccgggccc tcggagcgcg gacgatcgtg gagagcgtct tccccgccct gtgggagccc
      841 atggcgcggg caggtgtcac gctgtgcccg aagttgctca aggaagacat ccacccggtc
      901 gtgctggcca cggcgctggg ggcggcgaag cagtacggcg ctgacctgtg gttcagcccg
      961 gacctgtggt acaccgaccg cttcccgggg cactcggtgg agtcgtaccg cgcggcgcta
     1021 gagctcgcgt atcaggcggg cgtggaccac gtctactcgg agcagatcgt tgccatgtgc
     1081 cgtgtgcgcg gcgaggtgta tgacgtcacc ggacacggcc gggcactgcg ggagttcatc
     1141 gaccgcttgc gggagcggcc ggagcgccct tacgcgtacc gcgactacgc acccgaggtg
     1201 gccatcatcc acttccccga cagcgactgg ggtcaggcgt cgtgctacta ctgggacaca
     1261 ctctacggcg ccgagaacct gccgccgacg gctgagacgg ccgagtggat gcaggtgtgg
     1321 tcgttgctga cagagggggc cagcgatccg cgcgcggtga acacgaacag cagcgtctat
     1381 ccgatgggca gttggcgctt caccatgccc tgcatgcccg tggccgtgta cggccatcgg
     1441 gtgggggacg ggccgctcga gggcgtgaag acactgttcc tggccggcct ggatcttccg
     1501 cggccaacgc tcgaggcagt aggtcgccgg gtgcgggcag gcgcagtgtg cttcgcgccg
     1561 agacggcagt gcccagaggg cgtcgcggca cgggccgcgg cgctgcccat gcgcgtgccc
     1621 gacgggcggg gctcgtggat cgtcgtcgac gggttcgcgc ccgagcaact cggcgagtac
     1681 ctgcggctcg tgcccacgac gcacgagggg atgcacttgc ggtttggcga gcacgcggtg
     1741 cgcatcggct ccgtggccga tgagccatag gtcggagctc gtggctgggg tctggaggag
     1801 gcatgcagtg gacgtgagca cactgactcg cctggtggtg ctgagcgggc tggcatgtgc
     1861 cgcgggaggc cagggcctga gctcgcccga ggtcgtcctc cgggactggt acttccgcga
     1921 gtccctggtg gcaatccggg gttgggaggt gctgctcgcc gagtgggagc agcagacgcc
     1981 ggaggaccag cgcatcccga cgcttccggt gcccgatggc gcgatcgtcg agtgggccaa
     2041 tcccatcccc ctcacgctag tgccccccga ggacc
//
LOCUS       DAOSPL010000919         2073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_53844_length_2073_cov_3.393459, whole genome shotgun sequence.
ACCESSION   DAOSPL010000919 DAOSPL010000000
VERSION     DAOSPL010000919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53844_length_2073_cov_3.393459"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PLQ54_15205"
     CDS             complement(<1..418)
                     /locus_tag="PLQ54_15205"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOS94667.1"
                     /translation="MSRLGWYVIYTHTGQEAKVKANIERMIQQADLRDRVRRVEVPME
                     EEVRMVGGKRKSISRKVFPGYVLVQIAMDDETYHLLRNTAGVTGFVGAPHGSARRPTP
                     LSDAEVREILKTLGDEPRRVKPAYQRGETVLVLSGPF"
     gene            complement(499..729)
                     /gene="secE"
                     /locus_tag="PLQ54_15210"
     CDS             complement(499..729)
                     /gene="secE"
                     /locus_tag="PLQ54_15210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOS94668.1"
                     /translation="MGTAEGIAPVRWFRSFRGFIREATAELKRVVWPTWDELKGYTYL
                     VLLVIAVVAVWLGLLEFVLAWLTNKVGLYGRG"
     gene            complement(805..877)
                     /locus_tag="PLQ54_15215"
     tRNA            complement(805..877)
                     /locus_tag="PLQ54_15215"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(842..844),aa:Trp,seq:cca)
     gene            complement(917..1078)
                     /gene="rpmG"
                     /locus_tag="PLQ54_15220"
     CDS             complement(917..1078)
                     /gene="rpmG"
                     /locus_tag="PLQ54_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOS94669.1"
                     /translation="MASDKFWLACSVCGDYNYVTCKNKQNTQGKMKLMKYCPRDRKHT
                     EHNETRLRD"
     gene            complement(1115..>2073)
                     /gene="tuf"
                     /locus_tag="PLQ54_15225"
     CDS             complement(1115..>2073)
                     /gene="tuf"
                     /locus_tag="PLQ54_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOS94670.1"
                     /translation="PGHADYIKNMITGAAQMDGAILVVGADDGPMPQTREHILLARQV
                     NVPAIVVFLNKCDLVDDEELLELVELELRELLTKYEFPGDDIPIIRGSAYKAMTSTSD
                     DMSAPEYQPILDLMAAVDEHIPTPVRDVDRPFLMPIEDVFTISGRGTVVTGRIERGRI
                     TAGKDVEIVGIRETRKTVATDLEMFQKKLDYAEAGDNAGILLRGVERSEVERGQVLAA
                     PGSISPHRKFEAEVYVLSKDEGGRHTPFFTGYRPQFYFRTTDVTGTATLPEGVEMVMP
                     GDNIRMTIELIVPIAMDEGLRFAIREGGHTVGAGVVTKILDL"
BASE COUNT          410 a          766 c          516 g          381 t
ORIGIN      
        1 cgaaaggccc agagagcacc aacaccgtct ctccccgctg ataggcgggc ttcacacgcc
       61 ggggctcatc gcccaaggtc ttcagaatct cgcgaacctc agcatccgac agcggagtgg
      121 gccgccgagc actcccgtgg ggcgccccaa cgaaaccagt gacccccgct gtgttccgga
      181 ggaggtggta cgtctcatca tccatggcga tctgcaccag gacatagccc gggaagacct
      241 tgcgggagat ggacttgcgc ttgccgccca ccatgcggac ttcttcctcc atggggacct
      301 ccacgcgccg cacccggtcg cgcagatccg cctgctggat catccgctcg atgttggcct
      361 tcaccttcgc ctcttggccc gtgtgtgtgt aaatcacata ccagcccaag cggctcatac
      421 tctgtgcacc gccagtctgc ctccggcctt cctctgcatc catcactgcc cccgaaccga
      481 agaacgcgag ccgtccatct agcctcgccc atacaggccg accttgttgg tcagccaggc
      541 caaaacgaac tccaggagac ccagccagac cgctacgacc gcgatgacca ggagcacgag
      601 ataggtgtag cccttgagct cgtcccaggt aggccagacg acacgcttga gctccgcagt
      661 cgcctccctg atgaaccccc ggaagctgcg gaaccagcga accggtgcga tgccctctgc
      721 agtgcccata gatgcattcc tctgcgaacc cggaccgaat gccgaaacct accagtacgg
      781 ttccgggccg tccttgacgc tgggcaggcg agacaggact cgaacctgca acctgcggtt
      841 ttggagaccg ctgctctgcc agttgagcta ctcgcctgcg tcccacgagt ggggaaccgg
      901 ggcctcacgc aagaacctag tcgcgcagac gcgtctcgtt gtgctcggtg tgcttccggt
      961 cccgggggca gtacttcatc agcttcatct tgccctgcgt gttctgcttg ttcttgcacg
     1021 taacgtaatt gtaatcgccg cagacactac aggcaagcca gaacttgtcc gaagccatgg
     1081 ccaaacgtcc tcctcagcct tcgcccactc ggccctacag gtcgaggatc ttggtgacga
     1141 cgccggcgcc cacggtgtgg ccgccctcgc ggatggcgaa ccgcagaccc tcgtccatgg
     1201 cgatgggaac gatcagctcg atggtcatcc ggatgttatc ccccggcatc accatctcca
     1261 cgccctcggg aagcgtcgcc gtacccgtca cgtccgtcgt ccgaaagtaa aactgcggac
     1321 ggtaacccgt gaaaaacggc gtgtgacgac ccccctcgtc cttcgacaac acatacacct
     1381 ctgcctcaaa cttccggtgc gggctgatcg aacccggagc cgccaatacc tgaccccgct
     1441 ccacctccga ccgctccaca ccccgaagca agatccccgc gttgtctccg gcctccgcgt
     1501 agtccagctt cttctggaac atctccagat ccgtcgccac cgtcttccgc gtctcccgga
     1561 tccccacaat ctccacgtct ttgcccgccg tgatccggcc ccgctctatc cgtcccgtca
     1621 ccaccgtgcc ccgaccactg atcgtgaaca cgtcctcgat cggcatcaaa aacggacgat
     1681 ccacatcccg aaccggcgtc ggaatatgct catccaccgc cgccatcaaa tccaatatcg
     1741 gctgatactc cggcgcactc atgtcatccg acgtcgacgt catcgcctta tacgccgaac
     1801 cccgtatgat cggaatgtcg tcccccggaa actcatactt cgtcagcagc tcccgaagct
     1861 ccaactccac cagctccaga agctcctcat catccaccaa atcacacttg ttcaaaaaca
     1921 ccacgatggc cggaacattc acctgacgcg ccagcaagat gtgctcccgc gtctgcggca
     1981 tgggaccatc gtccgcaccc accaccaaaa tggcaccatc catctgcgcc gcgcccgtga
     2041 tcatgttctt gatgtagtcc gcgtgccccg ggc
//
LOCUS       DAOSPL010000920         2071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_53917_length_2071_cov_3.812004, whole genome shotgun sequence.
ACCESSION   DAOSPL010000920 DAOSPL010000000
VERSION     DAOSPL010000920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53917_length_2071_cov_3.812004"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2071)
                     /locus_tag="PLQ54_15230"
     CDS             complement(<1..>2071)
                     /locus_tag="PLQ54_15230"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOS94671.1"
                     /translation="ATVPFGCYQTLGDLWLDIDTPDRRIEGYRRELDLRRAVARVSYR
                     SGGIGFAREVFASAPAQALVVRLTCDRPGALSLAVRLDRPERYSTAPEGENGLVMSGQ
                     LYDGAEGTSGIRYVARLRVVLEGGTVQAEDDRLRIVDANAVTLILAAATDYRLDPPRY
                     RGSSPETATRETLQRAAATPYARLCGDATADHQQYFGRVELNLARSASSDLPTNDRLA
                     AFSATGDDPELAALLFQYGRYLLISSSRPGDLPANLQGIWATGIQTPWNCDYHININL
                     QMNYWPAETTNLSECTEPLTDLMLSLLEPGQRTARIQYGMPGWVAHTITNVWGYTSPG
                     EGLVWGANPTAGAWMCQHLWEHYRFTGDTSYLRRVYPLLREAARFFLSWLSEDPKTGR
                     LVSGPATSPENSFRTADGTVGCLSMGPSMDQEIVWELLGEFADASQILGRDADLRERS
                     LTARGRLLWPGITRDGRLREWAEDYDEPEPTHRHCSHLYGLHPGWQFTREGTPELFEA
                     ARRSLERRGDSGTGWSMAWKVCFWARLYDGDHAYRLLRNFLRLTGDQGFNYSDGGGVY
                     TSLLCAHPPFQIDGNFGVAAGMAEMLLQSHAGELHLLPALPSAWPEGSVRGLRARDGF
                     EVDIAWSRGQLTRANLRSDLGRRCVLRSADDARVLGPSGLVDCERLGPGLRAFDTARG
                     ALYRVYPT"
BASE COUNT          341 a          675 c          737 g          318 t
ORIGIN      
        1 gggtcgggta cacccggtag agtgctccgc gcgccgtgtc gaaggcccgg aggcccggcc
       61 cgaggcgctc gcagtcgacc aagcccgacg gccccagcac ccgcgcgtcg tcggcggagc
      121 ggagcacaca gcggcggccc aagtcgctgc gaaggttcgc gcgcgtgagc tgtccgcggc
      181 tccaggcgat gtcgacctcg aatccatcgc gggcgcggag accgcgcacc gagccttcgg
      241 gccaggcact ggggagcgcg ggcaggaggt gaagctcgcc cgcatggctc tgcaggagca
      301 tctcggccat gccggcggcg acaccgaagt tgccatcgat ctgaaacggt ggatgggcgc
      361 acagcaggct ggtgtagaca ccgccgccgt ccgagtagtt gaacccctga tcgcccgtga
      421 gccgcaggaa gttcctcagc aggcgatagg cgtgatcgcc atcgtacaag cgggcccaga
      481 agcatacttt ccatgccatg gaccagcccg tgcccgagtc gccacggcgc tcgagactgc
      541 gacgagccgc ctcgaacagc tcgggggtgc cctcgcgcgt gaactgccag ccaggatgta
      601 gcccgtagag gtgcgagcag tgccggtggg tgggctcggg ctcatcgtag tcctccgccc
      661 actcgcggag gcggccgtca cgtgtgatgc ccggccacag caggcgcccg cgggccgtga
      721 gcgagcgttc gcgcaggtcg gcgtcgcgtc cgaggatctg ggaggcatcg gcgaactcac
      781 ccaggagttc ccacacgatc tcctggtcca tgctggggcc catactgagg catccgacgg
      841 tcccatcggc ggtgcggaag ctgttctcgg gcgacgtcgc cgggccggag acgagccggc
      901 cggtcttggg atcctctgag agccacgaca ggaagaagcg cgccgcctcg cgcagcagtg
      961 ggtagacgcg gcggaggtag cttgtatcgc ccgtgaagcg gtagtgctcc cacaggtgct
     1021 ggcacatcca tgcaccggcg gtggggttcg cgccccagac cagcccctcg ccgggactgg
     1081 tgtagcccca gacgttcgtg atggtgtgag caacccagcc gggcatgccg tactggatcc
     1141 gcgcggtcct ctgccccggc tcgagcagtg agagcatgag gtccgtcaga ggctctgtgc
     1201 actcggacag gttggtggtc tcggccggcc agtagttcat ctgcaggttg atgttgatgt
     1261 ggtagtcaca gttccagggc gtctggatcc ccgtggccca gatgccttgc agattggcgg
     1321 gcaggtcgcc ggggcgtgag ctcgatatga gcaggtagcg accatactgg aacagcaagg
     1381 cggccagctc tgggtcatcg ccggtcgcgc tgaatgcggc cagccggtcg ttcgtgggta
     1441 gatccgagga cgccgaccgc gccagattca gctcgactcg cccaaagtat tgctggtgat
     1501 ccgccgtggc atccccgcag agccgggcat acggtgtggc ggcggccctc tggagtgtct
     1561 cacgggtggc agtctcgggc gagctgcccc ggtaccgcgg cgggtcgagc cgatagtcgg
     1621 tcgccgcagc cagaatgagc gtcacagcgt tcgcatccac aatgcgaagc cggtcgtctt
     1681 cggcctgaac cgttccgccc tcgagcacga cccgcaggcg agccacatag cgaatgcccg
     1741 atgtgccctc ggccccgtcg tagagctgac cggacatcac caggccgttt tcgccctcgg
     1801 gggctgtcga gtaacgttcc gggcgatcca gccgcacggc gagcgagaga gccccgggcc
     1861 ggtcacacgt gaggcgcacg accagtgctt gggcaggcgc gctggcgaag acctctcggg
     1921 cgaacccgat gccgccagag cggtaggaga cgcgggccac cgcccgacgc aggtctagtt
     1981 ccctgcgata gccctcgata cgtcggtcgg gggtgtcgat atcgagccac aggtcgccca
     2041 gggtctggta gcagccaaac ggtacggtgg c
//
LOCUS       DAOSPL010000921         2070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_53958_length_2070_cov_3.204963, whole genome shotgun sequence.
ACCESSION   DAOSPL010000921 DAOSPL010000000
VERSION     DAOSPL010000921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53958_length_2070_cov_3.204963"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..567
                     /locus_tag="PLQ54_15235"
     CDS             187..567
                     /locus_tag="PLQ54_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015414943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOS94672.1"
                     /translation="MAQRGEIYQCGKQGLLVEVLTPADCTLQCCGEEMAALTANTQDA
                     AVEKHVPAIERNGSTIRVQVGSVAHPMDPDHWIEFIEVLDGDCLQRKYLHPGDAPVAE
                     FTVCGSAVTVREHCNKHGLWKAEG"
     gene            612..>2070
                     /locus_tag="PLQ54_15240"
     CDS             612..>2070
                     /locus_tag="PLQ54_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOS94673.1"
                     /translation="MRCDLRLLVAALGVVWGSAACDAASDTTLQCLVCHETVTPGVFH
                     DWTASRHAKRALAEVLAEPEPSRRFGRLSATDVPESLRAASVGCAECHTLNPEKHADT
                     FDHNGFKVHTVVTPDDCATCHPVEREEYARNLMSHAKTNLEANPVYHSLADAANGPQR
                     LEGGRLVQDRPSAMTEADSCFACHGTTVGVEGTRSRNTELGEMDFPVLTGWPNQGVGR
                     VNPDGSAGACTACHTRHRFSMAVARQPYTCSQCHQGPDVPAYKVYMASKHGNLYASHG
                     NTDDYDALPWVPGRHISTPTCAVCHASMLSSPDGRVLSERTHAYSDRLPWRLMGLPYA
                     HAHPKSPDTTIIRNQAGLPLATELTGEPASEFLIGPEEQAVRLGRMRQVCLNCHAGGW
                     VGGHFAKLDHAIETTNQSTLTATQLLLQAWDSGLANGLAAGASLFDDEIERMWVEHWL
                     FYANSTRLAAAMAGADYGVFADGRWQLSRNIEAMHQ"
BASE COUNT          359 a          727 c          671 g          313 t
ORIGIN      
        1 gagacctggt ggtcgagcgg ggtctgttat cgtccgacga ggccaatcag ctgctcgatg
       61 tccgagcgat gacgatccct ccggatgaac cggatgctgg ccggcccggc tcgtgaggaa
      121 acggtgctgc cctgccgccc cgtcgggggc gggtcagaca acttgtccca tggaggagag
      181 agggaaatgg ctcagcgtgg cgagatctac cagtgcggta agcaagggct gttggtggag
      241 gtgctcactc ctgctgactg cactctccag tgctgcggcg aggagatggc ggcactcacc
      301 gccaacacgc aggatgcggc cgtcgagaaa cacgtccccg ccatcgagcg caacggatcc
      361 acgatcagag tccaggtcgg cagtgtcgcc catcccatgg accccgacca ctggatcgag
      421 ttcatcgagg tcctcgacgg cgactgcctg cagcgcaagt acctccaccc gggcgatgcc
      481 ccggtcgccg agttcaccgt ctgcggtagc gccgtcaccg tccgcgaaca ctgcaataag
      541 cacggtctct ggaaagctga gggctagctg cggtgcatat ccgcgagtcc ccgcgggagg
      601 cgtcgggctg tatgaggtgc gatcttcggc tactggtcgc cgccctcggc gtcgtctggg
      661 gctcggcggc gtgcgacgcg gccagcgaca ccacactgca gtgcctcgtg tgtcacgaga
      721 ccgtcacgcc cggcgtgttc catgactgga cggccagtcg ccacgccaag agggccctcg
      781 cagaggtgct ggctgagccc gagccatcgc ggcgattcgg gcggctttcg gccaccgatg
      841 tgcccgagag cctgcgggcc gcatcggtgg gatgcgccga atgccacacg ctcaacccgg
      901 agaagcacgc cgataccttc gatcacaacg ggttcaaggt ccacaccgtc gtgacgcctg
      961 acgactgcgc gacctgccat cccgtggagc gggaggagta cgcccgcaac ctcatgtccc
     1021 atgcgaagac caatctggag gcgaaccccg tctaccacag cctggccgat gccgccaacg
     1081 ggccccaaag gctcgaggga ggccgactgg tgcaggaccg cccctccgcg atgaccgagg
     1141 ccgactcatg cttcgcgtgc cacggcacca cggtcggcgt cgagggcacc cgctcgcgca
     1201 acaccgagct cggcgagatg gacttccccg tcctcacggg gtggccaaac cagggcgtcg
     1261 gccgagtgaa ccccgacggc agcgccgggg cctgcaccgc ctgccacacg cgccaccggt
     1321 tctccatggc cgtggcccgc cagccgtaca cgtgctccca gtgccaccag gggcccgatg
     1381 tgcccgccta caaggtctac atggcgagca agcacgggaa cctgtacgcc tcacacggca
     1441 acacggatga ctacgatgca ttgccgtggg taccggggcg gcatatcagc accccgacct
     1501 gcgccgtgtg ccatgcgtcc atgctctcct cgcccgatgg ccgagtgctc tcggaacgca
     1561 cccatgccta cagcgaccgc ctgccctggc gcttgatggg cctgccctat gctcacgcgc
     1621 atccgaagag cccggacacg accatcatcc gcaaccaggc agggctgccg ctggccactg
     1681 agctcacggg ggagcccgcc tccgagttcc tcatcgggcc cgaggagcaa gcggtgcggc
     1741 tcggccgcat gcggcaggtc tgtctcaact gccatgccgg cggctgggtc ggtggccact
     1801 tcgccaagct cgatcacgcc atcgagacca ccaaccagtc caccctgacc gccacgcagc
     1861 tcttgctgca ggcgtgggac agtgggctgg ccaacggcct cgccgcgggc gccagcctgt
     1921 tcgacgacga gatcgagagg atgtgggtcg aacactggct gttctacgcc aactcgaccc
     1981 ggctggccgc ggccatggcc ggcgccgact atggtgtgtt cgccgacggc cgctggcagc
     2041 tgtctcgcaa catcgaggcc atgcaccagt
//
LOCUS       DAOSPL010000922         2068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_54024_length_2068_cov_3.189270, whole genome shotgun sequence.
ACCESSION   DAOSPL010000922 DAOSPL010000000
VERSION     DAOSPL010000922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54024_length_2068_cov_3.189270"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PLQ54_15245"
     CDS             <1..332
                     /locus_tag="PLQ54_15245"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOS94674.1"
                     /translation="GFVAELWVLIGTYNAGTGVGGSTALLGTPWFAYLAVGGILLAAA
                     YLLWTVQRVLMGDLKPDWAAMKDMTRLEVACLAPLMAGTLWLGCYPGPLLAFILPSAK
                     AISQWVI"
     gene            358..1881
                     /locus_tag="PLQ54_15250"
     CDS             358..1881
                     /locus_tag="PLQ54_15250"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOS94675.1"
                     /translation="MLFLFPELILCGLGLGATVAALFCRGRGAGRWALYGFFVCLAGV
                     VTGLCLLAYAIDLGPEATGQLTPEFIARTFAVDPLSLFFSTLALAVCGLAMLFSMEFV
                     EEVLEEHPAEFLALIAFASLGIACVARSRDLITLFFSLELVSISGYLLSGMLRREPRS
                     LEAALKYFLIGAVSSAIMLFGMSLIYGLTGATDLRVLRDAVGEGHLHAVGQLAGVRMV
                     SSQDPMVITGILCIVVGLGFKIAMVPFHMWCPDVYEGAPTPATAFLSVGPKAAGFVAI
                     VRLLMLTFAPSSGPVPWAQPVLWLCALTMTLGNVVACRQTNLKRMLAYSSIAHSGYIL
                     IAVVSLGSADLALPAIVMYLIAYVLMNVGAFSVVIALSNIEGREDIHAYNGLSKRSPY
                     LAYAWVIFAMSLAGVPPTAGFLGKLYVFMAALDDRVRLYWLAVVGVANAVISVWYYFR
                     VARHMFFVEGVRTEPIRVGRGAGWAIGLSLAGTLVLVPLFPWLISFATSAAQVLGAW"
BASE COUNT          230 a          684 c          709 g          445 t
ORIGIN      
        1 gcggtttcgt cgccgagctg tgggtgctga tcggcacgta caacgccggc accggagtcg
       61 gaggctcgac ggctttgctc gggacgccgt ggttcgccta cctggccgtg gggggcattc
      121 tgctggcggc ggcctatctg ttgtggaccg tccagcgggt gctgatgggc gacctgaagc
      181 cggattgggc cgccatgaag gacatgaccc ggctggaggt ggcgtgcctg gcgccgctga
      241 tggccggcac gttgtggctg gggtgctatc cgggtccgct gttggcgttc atcctgccca
      301 gtgcgaaggc gatctcgcaa tgggtgatct agtttggcga gcgaaggtgg cgcggggttg
      361 ctgttcctgt ttcccgagct gatcttgtgc ggactgggcc ttggggccac ggttgccgcg
      421 ctgttctgtc gcggccgcgg cgcgggccgc tgggcgctct atggcttctt cgtctgcctg
      481 gccggcgtcg tgacgggtct gtgcctgctt gcctacgcca tcgacctggg ccctgaggcg
      541 acgggccagc tcacgcccga gttcatcgcc cgcacgttcg cggtcgatcc gctgtccctg
      601 ttcttctcga cgctggccct ggccgtgtgc ggtctcgcca tgctgttctc catggagttc
      661 gtggaggagg tgctcgagga gcacccggcc gagttcctgg ccctcatcgc cttcgcttcg
      721 ctgggcatcg cctgcgtcgc gcgctcgcgc gacctgatca cgctgttctt ctcgctggag
      781 ctcgtctcga tctccggcta cctcctatcg gggatgctcc ggcgcgagcc ccggtcgctc
      841 gaggcggctc tgaagtactt cctgatcggt gccgtgtcgt cggccatcat gttgtttggg
      901 atgtctctga tctacggtct cacgggtgcc accgaccttc gcgtgcttcg cgatgcggtc
      961 ggcgagggcc acctgcatgc ggtcggccag ctcgccgggg tgcggatggt ttcctcccag
     1021 gaccccatgg tcatcaccgg catcctctgc atcgtcgtgg gcctcgggtt caagatcgcc
     1081 atggtgccct ttcacatgtg gtgtcccgac gtgtacgagg gagcgcccac gccggccact
     1141 gcgtttctct ccgtcgggcc caaggccgcg ggctttgtcg ccatcgtgcg cttgctgatg
     1201 ctgaccttcg cgccttcatc gggcccggtg ccctgggccc aaccggtgct gtggctctgc
     1261 gcgctcacca tgacgctcgg caacgtggtg gcctgccggc agaccaacct caagcgcatg
     1321 ctcgcctact cgagcatcgc ccacagcggc tacatcctca tcgcggtggt cagcctcggc
     1381 tccgcggatc tggcgctccc ggccatcgtg atgtacctga tcgcgtacgt gctgatgaac
     1441 gtgggggcgt tctcggtggt cattgccctc tcgaacatcg agggccggga ggacatccac
     1501 gcctacaacg ggctttcgaa gcgctcgccg tacctggcct atgcctgggt catcttcgcc
     1561 atgtccctgg cgggtgtccc ccccacggca ggcttcctcg ggaagctgta tgtcttcatg
     1621 gcggccctgg atgaccgcgt gcggctgtat tggctggccg tggtgggtgt ggcgaatgcg
     1681 gtgatctcgg tctggtacta ctttcgcgtg gcgcggcaca tgttcttcgt cgagggggtg
     1741 cgtaccgagc ccatccgcgt gggccgagga gcgggttggg ccatcggctt gtcgctggcc
     1801 ggcaccctcg tgttagtccc cttgtttccg tggctcatct cattcgccac cagcgccgcc
     1861 caggtgctcg gcgcctggta gggccggcgc gacgatcccg ggcgcgttgg gggcccaccg
     1921 caaccaggtg agcccgtctc gtggaggggc ccctgaccgg cccggttcga gcccccagaa
     1981 tcgggcgcgt tgggggccca ccacgctggg agaacgcgtg gttcccccca tggcgcccct
     2041 ccgcaagacg gtcaacgctc ggcgtgtc
//
LOCUS       DAOSPL010000923         2066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_54090_length_2066_cov_3.654401, whole genome shotgun sequence.
ACCESSION   DAOSPL010000923 DAOSPL010000000
VERSION     DAOSPL010000923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54090_length_2066_cov_3.654401"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2066
                     /locus_tag="PLQ54_15255"
     CDS             <1..>2066
                     /locus_tag="PLQ54_15255"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS94676.1"
                     /translation="LEAAGGGQILASEIAVNLADDETSRELSPLDLGQRHLRGIAKPM
                     GVYLISPPGASPSTGAPSRDLGFMPTNLGAQMDEFVGRDRELRELASRLSEPSTRLLT
                     VTGPGGVGKTRLIQHLGMAVVPSFEHGAWFIELAGVREPLQVPAAVAATLRVPLTGPP
                     ETQASELASHLSSRELLLLLDNFEHLVEGAATIGHVLAAAPRLKCVVTSRERLRLRGE
                     HVFQLSPLELPDAAASLEEQLASESMRLLATRVAEVRGRPLEPDEADEAARICRRVAG
                     LPLALELAASALSHMTLGELAQSLDEHIALPQAHLRDVPDRLGSMTAAIDWSISLLSD
                     EEALVLRQCAVFEGGFYVEAAEAVCSTRETRRALDALRDKSLLGVEARRGRTRYTMLP
                     VIREHCLALLADSAETCRARAALHFASVARQLDLVGPGQARALESARNDLDNIVAALE
                     WARARGADDMIRDIVWGMWPIFWMANRPSEGWDWLGLAIGASRRLGDDRALATLLIRT
                     AAVCKWRSDYDGMRARLAEGSEVADRTGLPRVCLLRDLEAASLALIDGDPEEARRLYG
                     SCLVSYTALGDPWGMVQSLKGLAEVELEQGDFVAAEYYTREALSLETPDTQPWAASNT
                     LYELGRVQMAAGDSAGAARSYAEAAGLCRAIGDAPGLFSRLRALARARQAAGDLEGAL
                     EAWDEA"
BASE COUNT          286 a          693 c          744 g          343 t
ORIGIN      
        1 cctggaagca gcgggtggcg gtcagatcct cgcctctgag atcgcggtga accttgccga
       61 tgacgaaacc tcccgcgagc tcagccctct cgacctgggt cagcgccacc tgagaggcat
      121 tgcgaagccc atgggcgtgt acctgatttc gcctcccggc gcgagcccgt cgactggcgc
      181 gccctcgcgt gatctgggct tcatgcccac caacctgggt gctcagatgg acgagttcgt
      241 cggccgcgat cgtgagctgc gcgagctggc gtctcggctg tccgagcctt cgacccgcct
      301 gctgacggtg acgggacccg ggggggttgg caagacacgg ctcattcagc acttgggcat
      361 ggccgtcgtc ccgtcgttcg agcatggcgc ctggttcatc gagctggcgg gtgtgcgcga
      421 gcctttgcaa gtgcccgccg cggtcgccgc gactcttcgg gttccgctga ccggtccccc
      481 ggagacccag gccagtgagc tcgcatccca cttgtcgagc cgcgagctgt tattgctgct
      541 cgacaacttc gagcacctgg tggaaggcgc ggccaccatc ggccacgtgc tcgcagcggc
      601 cccacgcctc aagtgcgtgg tgacctctcg cgagcggctg cggctccgtg gcgagcacgt
      661 gttccagctc tcgcccctgg aactgcccga tgcggcggcc agcctcgagg agcagcttgc
      721 cagcgagagc atgaggctcc tggcgacgcg cgtggcagag gtgcgtgggc ggcctctgga
      781 gcccgatgaa gccgacgagg ccgcgcgtat ctgccgccgg gttgctgggt tgccgctggc
      841 tctcgaactg gccgcgtcag cgctgtcgca tatgactctg ggcgagttgg cccagagcct
      901 cgacgaacac atcgccctgc cccaagccca tctgcgcgat gtgcccgacc ggctgggcag
      961 catgaccgcg gccatcgatt ggtccatcag tctgctgagc gacgaagagg cgctggtgct
     1021 tcgccagtgc gcggtgttcg agggcgggtt ctacgtcgag gcggcggagg ccgtctgctc
     1081 gacgcgggag acgcggcggg cgctggatgc cctgcgggac aagtcgctgc tcggagtcga
     1141 ggcgcggcgc ggccggacgc gctacaccat gctaccggtc atccgtgagc actgcctggc
     1201 tctgctggcg gactcggcgg agacgtgtcg cgctcgggcg gccctccact tcgcgagcgt
     1261 ggcacgccag ctcgacctgg tggggcccgg ccaggcgcgc gcattggagt cggctcgaaa
     1321 cgacctggac aacatcgtcg ccgccctcga gtgggccagg gcccgcggcg ccgacgacat
     1381 gatccgcgac atcgtgtggg gcatgtggcc gatcttctgg atggcgaacc gtccttctga
     1441 ggggtgggac tggctcgggc tggccatcgg cgcgagtcgt cgtctcgggg acgaccgggc
     1501 cctcgcaacc ttgctgatcc gcacggccgc ggtctgcaag tggaggtccg actacgacgg
     1561 catgcgggct cgactggccg agggctcgga agttgctgac cgcacgggcc tgccccgtgt
     1621 gtgtctgctg cgagacctcg aggcagccag tctggccctg atcgatggcg atcccgagga
     1681 agcgcggcgc ctctatggca gttgcctcgt gtcgtacacc gctctcggcg atccctgggg
     1741 catggttcag agcctcaagg gcctggcgga agtggaactg gagcagggcg acttcgtcgc
     1801 cgccgagtac tacacgcgcg aggcgctctc cctcgagacg ccggatacgc agccctgggc
     1861 cgcttcgaac acgctctacg aactgggccg agtgcaaatg gccgccggcg actccgcggg
     1921 cgcggcccgg agttacgcgg aagccgccgg tctgtgccgc gccatcggtg atgccccggg
     1981 cttgttcagc cggctgcggg ccctggcccg ggcacgccag gctgccgggg acctcgaggg
     2041 ggcgctcgaa gcctgggacg aggcgg
//
LOCUS       DAOSPL010000924         2066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_54107_length_2066_cov_2.737941, whole genome shotgun sequence.
ACCESSION   DAOSPL010000924 DAOSPL010000000
VERSION     DAOSPL010000924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54107_length_2066_cov_2.737941"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2066)
                     /locus_tag="PLQ54_15260"
     CDS             complement(<1..>2066)
                     /locus_tag="PLQ54_15260"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOS94677.1"
                     /translation="SQDGQTWQTVRRVSAVAISGAASQGQDAAPRLPGPPPPPKRDGG
                     AWRTGGLSASSLAAPRQDGRGLPNLALAPDARALASSVYAGGAMPIHQIRHLNDGWAG
                     NDHSWIANTGGPEWAEIDLGADYWICRVALGNDNSGRFGDRGATTYAILTAREHTEDS
                     SAPQWTRVAQRNGAPLLLRTEITFAPVQARYVRVLLEGSSGEPRLDEVEVYGRPTPLA
                     TADVGPLTPEEQPEPTGVTDALLKYAFVGEEHAWLKTYGRADLDPSLVPYNGRVQQYP
                     RHVGDDALPLPRLADGPVVDGELDDAAWALSSRGVARVADPRDFDQGPLVECAVRAGI
                     AGSDLALAIHVDRLLSSHVAVVSAADWQGCGIVTWTEGGLVFRTFTPQGEPAESRPIE
                     GACSDDLTGFELRIPLEWLPGAAGRGVRVGLGLGGLHTPAAGRPVYFRPSDLAVAEEG
                     PCLHGCFRLRLTNTGERPVDVTGTAPALRGGLTLGPGEVRVLDLASASGPIGPELDVT
                     LVEPGREAYELHLFRYDPLERTLDLMEGIADRLEAAGLEVARERRQVSELRARQEELL
                     AAAPDRSAERQVLWEARVAKRDLLLRDPDLAVLDRVLFAKRFAYWPSHIYTDYTDAPF
                     RPGGGVYALSIPRPDGRLAPERGQLVELFDGRNGIVRDPAPTFDAQRIYFGYRPSADG
                     FYHIMT"
BASE COUNT          327 a          737 c          712 g          290 t
ORIGIN      
        1 ggtcatgatg tggtagaagc cgtcggccga ggggcggtag ccgaagtaga tgcgctgcgc
       61 gtcgaaggtc ggggccgggt cgcgcacgat gccgttgcgg ccatcgaaca gctcgacgag
      121 ctggccccgc tcaggcgcca ggcggccgtc gggccgtgga atcgacagcg cataaacgcc
      181 gccgcccggt cggaaaggcg catcggtgta gtcggtgtag atgtgtgagg gccagtacgc
      241 gaaacgtttg gcgaacagaa cacggtccag cacggccaga tcggggtcgc gtaacagcag
      301 gtcgcgtttc gccacacgcg cctcccacaa cacctggcgc tcggcgcttc gatcgggtgc
      361 ggcggcgagc agctcctcct ggcgcgctcg aagctcagac acctgccgcc gctcgcgggc
      421 gacctcgagt ccggccgcct cgagccggtc ggcgatgccc tccatcaggt ccagcgtgcg
      481 ctccaggggg tcgtagcgga acaggtgcag ctcgtaggcc tcacggcccg gctcgaccag
      541 tgtcacgtcc agctccggtc cgatgggacc acttgccgac gccaggtcga gcacgcggac
      601 ctcacccggg cccagagtca gcccgccgcg aagcgccggc gcggtgccgg tgacatcgac
      661 cggccgctcg cccgtgttcg tgagccgaag ccggaagcaa ccgtgcagac acgggccttc
      721 ctcggcgacg gcaagatccg acggccggaa gtagaccggc cgtcccgcgg ccggagtatg
      781 aaggccgccc aagcccagac cgacccggac accgcgtccg gccgcccccg gcaaccactc
      841 gagcggaatc cgcagttcga atccggtgag gtcatcactg cacgcaccct cgatgggccg
      901 cgattcggcc ggctcgcctt gaggcgtgaa cgtgcgaaac acgaggccgc cctcggtcca
      961 ggtcacgatg ccgcagccct gccagtcggc ggcggacacg accgccacgt gcgagctcag
     1021 taggcggtcg acatggatgg ccaatgccag atcgctgccg gcgattccgg cccgcaccgc
     1081 gcactcgacc agggggccct gatcgaagtc gcgcgggtcg gccacgcgcg cgacgcctcg
     1141 cgaggagagt gcccacgcgg catcgtccaa ctcgccatcg acaaccgggc catcggccaa
     1201 ccgtggtagt gggagggcgt catcgcccac atgacgcggg tactgctgga ctcgaccatt
     1261 gtaggggacg aggcttgggt ccaggtccgc gcggccatag gtcttcagcc aggcgtgctc
     1321 ctcgccgacg aacgcgtact tcagcagtgc gtcagtgaca ccagttggct cgggttgctc
     1381 ttcgggcgtc agcggcccca cgtcggcggt cgcaagcggc gtcggccggc cgtagacctc
     1441 gacctcgtcg agtcgcggtt cgccactcga cccctcaagc agcacgcgga cgtagcgcgc
     1501 ctggaccggc gcgaaggtga tctccgttcg aagcagtagc ggcgccccat tgcgctgggc
     1561 cacgcgggtc cactggggcg cggacgagtc ctcagtatgc tccctcgccg tgaggattgc
     1621 gtacgtggtc gcccctcgat cgccgaagcg ccccgagttg tcattcccca gcgcaacgcg
     1681 acagatccag tagtcagcgc ccaggtcgat ctccgcccac tccggccccc ccgtgttcgc
     1741 gatccacgag tggtcattcc ccgcccaacc gtcattgagg tgccggatct ggtggatcgg
     1801 catggcgccg cccgcgtaga ccgacgaggc caacgccctg gcgtcgggtg ccagggccag
     1861 gttgggcaga ccccgcccgt cctgcctcgg tgctgccagc gacgaggccg acagaccgcc
     1921 cgtgcgccag gcgccgccat ctcgcttcgg cgggggcggg gggcccggaa gccgcggggc
     1981 cgcgtcctgt ccctgggaag cagcgccgct gatggccact gccgacaccc ggcgcacggt
     2041 ctgccaggtc tggccatctt gggaga
//
LOCUS       DAOSPL010000925         2065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_54132_length_2065_cov_3.402985, whole genome shotgun sequence.
ACCESSION   DAOSPL010000925 DAOSPL010000000
VERSION     DAOSPL010000925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54132_length_2065_cov_3.402985"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PLQ54_15265"
     CDS             <1..203
                     /locus_tag="PLQ54_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP synthase F0 subunit A"
                     /protein_id="HOS94678.1"
                     /translation="YGNVGGEDKILHVLAAMFLVAIPLHWPLTAFAVFTGFLQAFVFV
                     GLVCAYLQGTMAHHHEEHAEAQ"
     gene            237..509
                     /gene="atpE"
                     /locus_tag="PLQ54_15270"
     CDS             237..509
                     /gene="atpE"
                     /locus_tag="PLQ54_15270"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOS94679.1"
                     /translation="MDYPAALAIGVCLGLPIAVLSAALGQGKAAAAAMEGIARQPDAA
                     GDIRGSLIISLALIESLVIYALLIFFLMFSKLPSVMDYLGKVSGAQ"
     gene            622..1134
                     /gene="atpF"
                     /locus_tag="PLQ54_15275"
     CDS             622..1134
                     /gene="atpF"
                     /locus_tag="PLQ54_15275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOS94680.1"
                     /translation="MAELAQSLGLDPKLILSQIFAFLFTYYMLSRFLFRPIARMVDER
                     NREIVDRMARARQHEAEMEEKRQDYERRIADIEREARDRIQQATREAHEAGAQIVAEA
                     RQAREALLSQAREEIVREKERALVEIRDRVADLAVGGAERIIRHELNDERHRELVRHF
                     MDEVEHVGSS"
     gene            1136..1702
                     /gene="atpH"
                     /locus_tag="PLQ54_15280"
     CDS             1136..1702
                     /gene="atpH"
                     /locus_tag="PLQ54_15280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOS94681.1"
                     /translation="MRNDRASRRYAQALFSLAVDHGRIDEVSEDLDRAVRALDELPGV
                     RSLFTQPLMPDRIKRDLVDKVFGDSISQLTRDFLHVVVARNRGDLFDQIRERYIELCN
                     DYHGILPASVQSAVPLSESELAELSARLTRLCGKTVSPRCVVDPDLIGGVVVRMGDRV
                     MDGSVRGALRRLRQRLKGTDLSSLRPQA"
     gene            1725..>2065
                     /locus_tag="PLQ54_15285"
     CDS             1725..>2065
                     /locus_tag="PLQ54_15285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855266.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOS94682.1"
                     /translation="MALRPSEVTAVLRREIEQYQAQFDVAGVGTVLQVSDGVARIHGL
                     ADAMAGELLEFPGDVFGMALNLEEDNVAAVLLGSDVGIREGAEVKTTGRIVEVPVGKE
                     LLGRVVDALGRP"
BASE COUNT          339 a          644 c          730 g          352 t
ORIGIN      
        1 tctacgggaa tgtcggcggt gaggacaaga tcctccacgt gctcgccgcc atgttcctgg
       61 tggccatccc gcttcactgg ccgctgacgg ccttcgcggt gttcaccggg tttctgcagg
      121 cgttcgtgtt cgtcggactg gtgtgcgcgt acctccaggg cacgatggcc catcatcatg
      181 aggaacacgc cgaggcgcag tagcggcgtg gtaagcagtc gagaggggtc gttctcatgg
      241 actatccggc agcattggcg atcggcgtgt gcttggggct tcccattgcg gtcctcagcg
      301 ccgcgttggg gcagggcaag gcggccgccg cggccatgga aggcatcgcg aggcagccgg
      361 acgcggctgg cgacatcagg ggctcgctga tcatctcgct ggccctcatc gagtcgctgg
      421 tcatctatgc gttgctcatc ttcttcctga tgttctcgaa actgccgtcg gtcatggact
      481 acctgggcaa ggtgtccggc gctcaatagc cgtccctggc gagttgccct ggtccgggcc
      541 cggaccggag agctgtcggt gcgacgccac cggtggtacg ggtaggccca tgggtccgcc
      601 atcggcctag gaggcttgga gatggcagag ctagcccaga gcctggggct cgatcccaaa
      661 ctgatcctgt cccagatctt tgccttcttg ttcacctatt acatgctgag ccggttcctg
      721 tttcgcccca tcgcgcgcat ggtcgatgag cggaaccggg agatcgtcga tcgcatggcg
      781 cgggcacggc agcacgaggc cgagatggag gagaagcgcc aggactacga gcgccgaatc
      841 gccgacatcg agcgcgaggc ccgcgaccgg atccagcagg ccacccgtga ggctcacgaa
      901 gcgggggcgc agatcgtggc cgaagcgcga caggcccgcg aagcactcct cagccaggct
      961 cgcgaggaga tcgtgcgcga gaaggagcgg gcgctggtcg agatccgcga ccgcgtggcc
     1021 gacttggccg tcggcggcgc cgaacgcatc atccgccacg aactgaatga tgagcggcac
     1081 cgggaactcg tccggcattt catggacgag gtcgagcacg tggggagctc atgacgtgag
     1141 gaacgaccgg gcatcgaggc ggtacgccca ggctctcttc agcttagccg tggatcacgg
     1201 ccggatcgac gaggtgtctg aagacctcga tcgcgccgtg cgtgcgctcg acgagttgcc
     1261 cggtgtgcgc tcgctgttca cccagccgct gatgcccgac cgtatcaagc gcgatctggt
     1321 ggacaaggtg ttcggggact ccatctctca actgacccgc gatttcctcc acgtggtcgt
     1381 cgcgaggaac cggggcgacc tgttcgacca gatacgcgag cggtacatcg agctctgcaa
     1441 cgactaccac ggcatactgc ccgcctcggt gcagtcggcc gtgccactct ccgagtcgga
     1501 actggccgag ctgtcggccc ggctgacgcg gctgtgcggc aagaccgtta gcccaaggtg
     1561 tgtggtcgac cccgacttga tcgggggcgt ggtggtccgg atgggcgacc gagtgatgga
     1621 cggtagtgtc cggggggccc tgcggcggct gcgacagcgc ctgaagggca ccgacctgag
     1681 ttcactgcgg ccgcaagcat aaccacggac aggaggggcc cggcatggcg ctgcgcccca
     1741 gcgaggtgac cgccgtcctg cggcgggaga tcgagcaata tcaagcccag ttcgacgtgg
     1801 cgggcgtcgg caccgtcctt caggtgagcg acggcgtcgc ccggattcac ggactggccg
     1861 acgccatggc cggcgagctc ctggagttcc ccggagacgt cttcggtatg gcgctgaacc
     1921 tggaggaaga caacgtggcc gcggtcctcc tcgggtccga cgtgggcatc cgcgaagggg
     1981 ccgaggtcaa gaccaccggg cgcatcgtcg aggtgcccgt ggggaaagag ctgctcggcc
     2041 gggtggtcga cgccttgggc cggcc
//
LOCUS       DAOSPL010000926         2063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_54196_length_2063_cov_3.036853, whole genome shotgun sequence.
ACCESSION   DAOSPL010000926 DAOSPL010000000
VERSION     DAOSPL010000926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54196_length_2063_cov_3.036853"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..747)
                     /locus_tag="PLQ54_15290"
     CDS             complement(76..747)
                     /locus_tag="PLQ54_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94683.1"
                     /translation="MRLSKGAIVALIVGLVVVVLVTYFTAEIQNFVALKAWSKGATRA
                     VAARVVDAIQNRDAAAIQEFCGGPVKPTEADGKLVALGITGGMAPPPVPVENLTPVEG
                     VDKARIAFQIRPPHGTATVTMAGPNGSQIVLTLKPEKGKWNLVMVSSSVRAQNAAAGK
                     TVGPGAQKGAGKAPGGSGGPGGTSKGAGANGLGSKGPGAIGSPVGGGSPKGVPRAAPT
                     EAPAT"
     gene            complement(917..>2063)
                     /locus_tag="PLQ54_15295"
     CDS             complement(917..>2063)
                     /locus_tag="PLQ54_15295"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HOS94684.1"
                     /translation="GVMPYSHTYLDPRTNRHVPLTGVRLIEATRFLSTFPGDQLTSSV
                     PGHPVDVPAALQSLFQYKTGAQYNPQGGGYGWLGPPRVAEYVLRMAEEVGHPITATVV
                     YVDSPLRLGGHELRTAYELRHRFAEVHVGNMGSCGATLPLHPRTALALSWAEAILAGL
                     CVEFLTGLPTDWGGTLEPFDLRAQTIPFGAPEQALYYQMGREASAWLTGNRPHAGHAP
                     LLTMAKVPGAQAALEKGMSAALGVARGCTSLGAAGSLSADEVFSPAQVVLDLELRDWL
                     ERFAAGICRPEDEPADPLKAIREALSGRGFAAADATLDSYAALSWFPRHLRREMLDRW
                     RDLGSPSAEETAREEAIERIEGAQWTLPEPLFGALEAIYAEAAACLG"
BASE COUNT          319 a          727 c          681 g          336 t
ORIGIN      
        1 ttgctgatgc gcatgggatc acccttcggg tatgggaatg gagaacctca catggactgt
       61 tcggccccca gctagctatg tcgcgggcgc ctcggtgggc gccgcacggg gtacgccttt
      121 ggggctcccg ccgcccacgg gagagccgat agccccgggg cccttggatc ccagtccatt
      181 ggctccggct cccttgctcg tgcctccggg gcctccggaa ccgcccggcg ccttcccggc
      241 acccttctgc gcgccgggcc ccaccgtctt ccccgcggct gcattctggg cccggaccga
      301 ggaggagacc atcacgagat tccacttccc cttctcgggc ttgagggtca gcacgatctg
      361 gctgccgttc ggtcccgcca tcgtaactgt ggcggtgccg tggggcggac gaatctggaa
      421 cgcgatccgt gccttgtcga cgccctcgac cggtgtcagg ttctcgaccg ggacgggcgg
      481 gggcgccatg ccaccggtga taccgagcgc caccagtttg ccgtcggcct ccgtcggctt
      541 cacggggcct ccgcagaact cctggatggc tgccgcatcc cggttctgga tcgcgtcgac
      601 gacccgagcc gctactgccc gcgtcgcccc tttgctccac gccttcagtg ccacgaagtt
      661 ctgaatctcg gcagtgaagt aggtcacgag aaccacgacg accagcccga cgatgagagc
      721 cactatggct cccttgctga gacgcatgct cgccaacctc ctgagcccgg aagcctcgcc
      781 acccgcgccc gggagggatc agcggcacgg cgggtgacca tcggggtcga gacgttcacc
      841 ccatcaaggc attcggggac ccggcgccgc tctcctctgc gcagacaggt tccgccttgg
      901 ggacggcggc gccgccttac ccgagacacg cggccgcctc ggcatagatg gcttcgagcg
      961 ccccaaacag cggctccggg agtgtccatt gggccccttc gatccgctcg atggcttcct
     1021 cccgcgccgt ttcctctgcc gaaggcgaac cgaggtcccg ccaccggtcc agcatctcac
     1081 ggcgcaggtg tcgggggaac cacgatagcg ccgcatagct gtcgagtgtc gcgtcagccg
     1141 cggcgaaccc tcggccggac aatgcctcgc ggatggcctt gagcgggtcc gcgggctcgt
     1201 cctcaggccg gcaaatgccg gccgcgaacc gctccagcca gtcgcgaagc tccagatcga
     1261 ggaccacctg agccggactg aatacctcgt ccgcactcag actacccgcg gcgcccagtg
     1321 aggtgcagcc ccgggcgaca ccaagagcgg ccgacatgcc cttctcgagc gccgcctgcg
     1381 cgcccggcac tttcgccatg gtgagcaggg gcgcatggcc ggcgtgcggc ctattgccgg
     1441 tcagccaagc tgaggcctcc cgtcccatct ggtagtacag ggcctgctcc ggcgcgccga
     1501 aggggatcgt ctgcgcacga aggtcaaatg gctcgagcgt tcctccccag tcggtcggca
     1561 atccggtcag gaactcgacg cagaggcccg cgagtatggc ttctgcccac gagagggcga
     1621 gggccgttcg cgggtggagc gggagcgtcg cgccacagct ccccatgttc ccgacatgca
     1681 cctcggcgaa acggtgccgc agctcatacg ccgtgcgaag ctcgtggcct ccgagtcgca
     1741 gcggggagtc gacgtagact actgtggcgg tgatcgggtg ccccacctcc tcggccatgc
     1801 gcagcacata ctcggcaact cttgggggac cgagccagcc gtacccgccg ccctgagggt
     1861 tgtactgggc tcccgtcttg tactggaaga gcgactgcaa cgcagccggt acatcgacgg
     1921 ggtgcccagg taccgacgag gtaagctggt cgccgggaaa cgtggacagg aagcgggtgg
     1981 cctcgatgag tcgcaccccg gtgagcggga cgtggcggtt cgtacggggg tccagatacg
     2041 tgtggctgta gggcatcacg ccc
//
LOCUS       DAOSPL010000927         2062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_54241_length_2062_cov_3.267065, whole genome shotgun sequence.
ACCESSION   DAOSPL010000927 DAOSPL010000000
VERSION     DAOSPL010000927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54241_length_2062_cov_3.267065"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="PLQ54_15300"
     CDS             <1..1414
                     /locus_tag="PLQ54_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94685.1"
                     /translation="WVRDHVHEMKAYKWWAEDLTSFIDTLIELQPARGFFHEILTVPT
                     DSHTTFVHPEFVRIEPGDSLAFVRLELEADVEYLMVEGVYTAWQATGDDAAMERRLAA
                     LEAGMQFCLEDPMRWDAEHQLMKRPLTPDTWDFTFGLSDQDRTVSDYTPMAIMHGDQS
                     GLYQACVQLAKMHRALGHEERAEEWQRQGEAIRARANALLWTGDHYLHQLYLQPVDTR
                     VDESQILSLSNTYDINRGMPTHEMAVRIIDAYHSRKASAQFAEWYALDPPYPQFGPYP
                     AGAYINGGVAGFVAGELAKAALAHGREAYGADIIDRVAQLVAERGMLGFLYTRDGQDQ
                     GGGPSGWSAAAVLSAMAEGLAGIVDDATEYEDITVAPRFAAAGVSEATVCLPYGPSKA
                     YVALQYSAGPDAIDLTVAGNARHIHLDALLPDGRELSRVEVDGERVGAAVRPIEGSRY
                     AQADVDARPPVTHIRVTLKP"
     gene            1654..1956
                     /locus_tag="PLQ54_15305"
     CDS             1654..1956
                     /locus_tag="PLQ54_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94686.1"
                     /translation="MGAPVGAVTSRERDGRGYTLNIDGGWWVAVNRMDGEPAIGSDRG
                     FPRSVHAEHRIETQPTSEQIDAPGRDLVAKVLPDFDIAEMESWFTMVPPRSADVGA"
BASE COUNT          357 a          690 c          708 g          307 t
ORIGIN      
        1 ttgggtgcgt gaccacgtgc acgagatgaa ggcctacaag tggtgggccg aggacctgac
       61 gagcttcatc gacactctca tcgagctgca gcccgcgcgc gggttcttcc atgagatcct
      121 caccgtgccc acagacagcc acaccacctt cgtgcacccc gagttcgtgc ggatcgagcc
      181 cggcgactcg ctggcgttcg tccggctcga gctcgaggcc gatgtggagt atctgatggt
      241 cgagggcgtc tacaccgcct ggcaggccac cggcgatgac gcagccatgg agaggcgcct
      301 cgccgcgctc gaggccggga tgcagttctg cctcgaggat cccatgcggt gggatgcaga
      361 gcaccagctc atgaagcgtc cactgacgcc cgacacgtgg gacttcacct ttgggctctc
      421 ggatcaggac cgcaccgtct cggactacac gcccatggcc atcatgcacg gcgatcagag
      481 cgggctctac caggcctgcg tgcagctcgc caagatgcat cgtgcattgg ggcacgagga
      541 acgggccgag gagtggcagc ggcagggcga ggccatccgc gcccgtgcca acgcactcct
      601 ctggacgggc gatcactacc tccaccagct ctacctgcag ccagtcgata ccagagtcga
      661 tgagtcgcag atcctgagcc tgtcgaacac ctacgacatc aaccgcggca tgccgacgca
      721 cgagatggcg gtgaggatca tcgacgccta ccactcgcgg aaggcctcgg cccagttcgc
      781 tgagtggtac gcgctcgacc cgccgtaccc gcagttcggg ccctatccgg ccggggcgta
      841 catcaacggc ggcgtagccg gcttcgtggc gggtgaactg gccaaggcag ccctcgccca
      901 tggccgcgag gcctatgggg cggacatcat cgaccgagtg gcccagctcg ttgcggagcg
      961 cggaatgctc gggttcctct acacgcgtga cggccaggac cagggcggcg ggccatcggg
     1021 ctggagcgcc gccgcggtgc tctcggccat ggccgagggg ctggcgggca tcgtcgacga
     1081 cgccacggag tacgaggaca tcaccgtggc gccacgcttc gccgccgcgg gcgtgtccga
     1141 ggccacggtg tgcctcccct atggcccctc gaaagcctac gtggccctgc agtactccgc
     1201 cgggccagat gccatcgacc tgaccgtggc cgggaacgcg cgacacattc acctcgacgc
     1261 actgctgccg gatggccggg agctgtctcg ggtcgaggta gacggcgagc gagtgggggc
     1321 agcggtccgg ccgatcgagg gcagtcgcta cgcgcaagcc gatgtcgatg ccaggccacc
     1381 cgtcacccac atccgcgtga cgctgaagcc atagagcaag gctcagggtg cacatcacac
     1441 cgcgtcggca ctgccgagca tggcccagcc ggcaactcgg tgggggaggc gaatacacgg
     1501 gtcctctgat cccgccgcca gtcatcccgc ccatctatgc cccgaacgag agcgcgactg
     1561 aggtcgacga ggactgccac tcgcggtact cgccgtatgt gtcgagcgag tgaggagcag
     1621 gcggtcgcca ctgcggtctc agccgcgaag cgcatgggag caccagtcgg cgcggtcact
     1681 tcgcgggagc gggatggcag gggctacacc ctcaacatcg atgggggctg gtgggtggct
     1741 gtgaaccgca tggacggtga gccggccatc gggtcagacc ggggctttcc caggtccgtt
     1801 cacgccgagc accggatcga gacccaaccc acgtctgaac agatcgacgc cccgggccgg
     1861 gacctggtgg ccaaggtcct ccccgacttc gacatagccg agatggagtc gtggttcacc
     1921 atggtgccgc cgaggtcggc cgacgtcggg gcctgatggc gccaggtgat cgctgagaac
     1981 ggctgccgtg cccccgggat gtgctcggcg gccggccacg gtgacggcgg agccagccga
     2041 ggagatcgtg cgtccgaagc tg
//
LOCUS       DAOSPL010000928         2062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_54246_length_2062_cov_2.814150, whole genome shotgun sequence.
ACCESSION   DAOSPL010000928 DAOSPL010000000
VERSION     DAOSPL010000928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54246_length_2062_cov_2.814150"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="PLQ54_15310"
     CDS             <1..1278
                     /locus_tag="PLQ54_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94687.1"
                     /translation="DISRGLVQLDAGEQERENVKAIQAVLKVLEDPVGGLLRVVELFE
                     ARKPKGMAIVTHLDGTVAKIDETGLRRVIIHSELPVDETNILRGEVAAEPVRTKNDEL
                     VVDEGEELGERQIKLLRRHKIKSVVIRRAYLVPYRGYLSVEEGMKVRAGDRLTEGPLD
                     PQQILAMRGVRAVQEYMVREIQRVYRGQDVDINDKHIETIVRQMLRKIRVIDHGDTDL
                     LPGQVVDRFIFEEENRRVQSLGGRPGQGEWMLLGITEASLQTESFLSAASFQKTTKVL
                     TEAATRGMIDPLRGLKENVIIGRLIPAGSGLATYRDTDLAPLPVSAVWHEFAGVDTAD
                     DRSFRLFSADEDEHELEDTEAVEVPGEGDEEPVEAVVDDDAEPSGAADEEELFADEGL
                     DEAEEEEGSDIAGLTVDDPDEDAPDASPDALDD"
     gene            complement(1368..>2062)
                     /locus_tag="PLQ54_15315"
     CDS             complement(1368..>2062)
                     /locus_tag="PLQ54_15315"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HOS94688.1"
                     /translation="REVVADYAVVGRDLRSFVLVFSLAVAAAFLPARLYKHAAAAMLV
                     VIYAIYVRRTFQGASAPEEGHDIAPLHLRRSSIIPRYRYVLCQLFVALAAIIGGAHLF
                     VHHLSEIAHATGVSALVLSVIITPIATELPEKFNSVLWVRRGKDTLALGNISGAMVFQ
                     SSIPPAVGLICTDWALTDRAIAAAVAAIAASGLALGEMMWRKRISAWSLLLGIVFYAA
                     YLAYVFSTPGVQ"
BASE COUNT          378 a          603 c          776 g          305 t
ORIGIN      
        1 gacattagcc gtggtctggt gcagctcgac gcgggagagc aggagcggga gaacgtcaag
       61 gcgatccagg cggtcctcaa ggtcctcgag gatccggtag gcggcctgct gcgggtggtg
      121 gagctcttcg aggctcgcaa gcccaagggc atggcgatcg tcactcacct ggatggtacc
      181 gtggcgaaga tcgatgagac gggcctgcgc cgggtcatca tccactccga gctgccggtg
      241 gacgagacga acatcctccg tggcgaggtg gcggccgagc ccgttcgcac gaagaacgac
      301 gagttggtgg tcgacgaggg cgaggagctt ggcgagcgtc agatcaagct actgcgccgg
      361 cacaagatca agtcggtggt aatccgccgg gcctacctgg tgccctaccg cggctatctg
      421 agtgtcgaag agggcatgaa ggttcgggcg ggggatcgcc tgacggaggg gcccctggat
      481 ccgcagcaga ttctggccat gcgcggggtg cgcgcggtgc aggagtacat ggttcgcgag
      541 atccagcgcg tgtaccgggg ccaggacgtg gacatcaacg acaagcacat cgagaccatt
      601 gtccggcaga tgctccgcaa gatccgtgtg atcgaccatg gggacacgga ccttctgccc
      661 gggcaggtgg tcgatcggtt catcttcgag gaggagaacc ggcgcgtgca gtcgctgggt
      721 ggccgtcctg ggcagggcga atggatgttg ctcggcatca ccgaggcttc cctccagacc
      781 gagagcttcc tctcggcggc ctcgttccag aagacgacca aggtgctcac cgaggcggcg
      841 acgcgcggca tgatcgaccc gctgcgcggg ctgaaggaga atgtgatcat cggccgactg
      901 atccccgcgg ggagcggctt ggccacgtac cgcgacacgg acctggcgcc actgcccgtg
      961 tcggccgttt ggcacgagtt cgcgggcgtg gacacggcag acgaccggtc cttccgcctg
     1021 ttcagcgctg atgaggacga gcacgagctg gaggacacgg aggccgtgga ggtcccgggc
     1081 gagggcgatg aggagccggt ggaggccgtg gtggatgatg acgctgagcc cagcggggcc
     1141 gcggacgagg aggagctgtt cgccgacgag ggcctcgatg aggcggagga agaggagggt
     1201 tccgacatcg ccggcctcac cgtcgacgac ccggacgagg acgctcccga cgcatcgccc
     1261 gatgcgctgg acgactaggc ttcccctcca cgagacgtct gcagggcccc ggcggctggt
     1321 ctgccggggc cctgtttttc tgggcggcgc gggacgcggg tttggctcta ctggacgccc
     1381 ggcgtggaga acacataggc gagataggcg gcgtagaaca cgatcccgag cagcagcgac
     1441 cacgcgctga tgcgcttgcg ccacatcatc tcacccaacg ccaggcctga agccgcgatg
     1501 gcggcgacgg ccgcggcgat ggcgcggtcg gtgagggccc agtcggtgca gatgaggccc
     1561 acggccggag ggatgctgct ctggaagacc atggcgccgc tgatgttccc gagggccaat
     1621 gtgtccttgc cgcggcgaac ccagaggacg gagttgaact tctcgggcaa ctcggtggcg
     1681 atgggagtga tgatgaccga aagcaccaga gcggatacgc cggtggcgtg agcgatctcg
     1741 gagaggtgat gaacgaacag gtgggcgcca ccgatgatgg cggccaaggc aacgaacagc
     1801 tggcagagca cgtaccggta gcgcggtatg atcgaggagc gcctcaggtg gagcggcgcg
     1861 atgtcgtggc cctcttccgg cgcgctggcg ccctggaacg tgcgccggac gtagatcgcg
     1921 tagatgacca ccagcatcgc ggcggcggca tgcttgtaga gccgagccgg caggaatgcg
     1981 gcggctacgg cgaggctgaa gacgaggacg aagctgcgca gatcacgccc cacgacagcg
     2041 tagtcggcga cgacctctcg gg
//
LOCUS       DAOSPL010000929         2055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_54479_length_2055_cov_3.030000, whole genome shotgun sequence.
ACCESSION   DAOSPL010000929 DAOSPL010000000
VERSION     DAOSPL010000929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54479_length_2055_cov_3.030000"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1921
                     /locus_tag="PLQ54_15320"
     CDS             104..1921
                     /locus_tag="PLQ54_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94689.1"
                     /translation="MLVDVLLTAAVSLATPRATYLQDMGGPVTFDWPFPARSTDDRLG
                     LVLGDAAIRATCESGGSVWAATDRGLAMVDGDRVRWSEASADGLLSSDCRDVAADRAG
                     MVWIATDRGVSVTDGHGHWLRLTGAEGLPVEDVRCIALGPTGDRWFGTSRGVCRLREG
                     EFSYFAGRRWLPSDDVLDLAVDRNGAAWVRTPSGVGQIASKPMTLEEKAERFLTRLRQ
                     RHIRHGLSVECRFESENDLTRYRLDASDNDGLWTALYVAAESFRYAVTRADDARRNAR
                     DSMMALVKLEEITGIRGFPARSMIRPGEDVNQSGGEWHPSPDGQWIWKGDTSSDELDG
                     HYFAYAIYYDLVADEEGRRILRETIARITDHLLDHEYSLIDLDGQPTRWAVFGPQALN
                     DSPQWAVERGLNSLSMLSHLNVAYHLTRNERYRRAHDELVREHGYALNAVTQKVLIPG
                     EVNHSDDELAFLCYYPALLYERDPDVRALLLASIERSWRIEQPERNPFFNFVYGAVTG
                     KPCDVEAAERTLREYPMEIRDWRMENSHRADIMVDPRSGRFDETQSTRVLPYSERCFL
                     KWNGNPYRLDSGGDGLSEDDGAAFLLPYWMGRYYGFVTE"
BASE COUNT          347 a          685 c          692 g          331 t
ORIGIN      
        1 ccctggcgcc ctcccgacga ctcccgtgcg caggcccggc tggagccagc ggcgaaccgg
       61 aggatggtgc gcccgcgggc gccacggtga ggagtggccc gccatgctcg tcgatgtgct
      121 gctcacagcg gccgtatcgc tggcaacccc cagagccaca tacctccagg acatgggggg
      181 cccggtcacc ttcgactggc cgttccctgc ccggtccacc gacgaccggt tgggcctcgt
      241 gctgggtgat gcggccatcc gcgccacctg cgagtctggt ggctccgtct gggctgccac
      301 cgaccgaggg ttggcgatgg tcgatggcga ccgcgtgcgc tggtccgagg cttcagccga
      361 cgggcttctc agcagtgact gccgggacgt ggccgccgac cgcgccggca tggtgtggat
      421 cgcgacggac cgcggcgtca gcgtcacgga tggccacggg cactggctgc ggctgaccgg
      481 ggccgagggt ctgcccgtcg aggacgtgcg ctgcatcgct ctcgggccaa ccggtgatcg
      541 atggttcggg acatcgcgcg gcgtctgccg gctgcgcgaa ggcgagttca gctacttcgc
      601 cggccgtcgc tggctgccga gcgacgacgt gctcgacctg gccgtcgaca ggaacggtgc
      661 cgcatgggtg cgcacaccgt ccggcgtggg ccagatcgcc tcgaagccca tgacgctcga
      721 ggagaaggcc gaacgcttcc tgactcggct gcgacagcgg cacatccggc atggactgtc
      781 ggtggaatgc cgattcgagt cggagaacga cctgacccgg taccggctcg acgccagcga
      841 caacgatggc ctgtggacgg ccctctacgt cgcggccgag agcttccgct acgcggtgac
      901 ccgggccgat gacgcccggc ggaatgcgcg tgactccatg atggctctgg tgaagctgga
      961 agagatcacg gggatccggg ggttcccagc gcggtcgatg atccgacccg gcgaggatgt
     1021 gaaccagtcg gggggcgaat ggcacccatc ccccgatggc cagtggatct ggaagggaga
     1081 cacgagctcc gatgagctcg atgggcacta cttcgcctac gccatctact acgatctcgt
     1141 ggccgacgag gagggccgca ggattctgcg cgagaccatc gcgcgcatca ccgatcatct
     1201 gctggatcac gagtactccc tcatcgatct cgatggccag ccgacgcgct gggccgtgtt
     1261 cggcccgcaa gcgctcaacg attcgcccca gtgggccgtc gaacgaggcc tgaactcgct
     1321 ctcaatgctc tcgcacctca acgtcgccta ccacctgacc cggaatgaac gctaccggcg
     1381 agcccacgat gagctcgtac gcgagcacgg ctacgcgctc aacgcggtca cgcagaaagt
     1441 gctcataccc ggcgaggtca accattcgga cgacgaactg gcattcctgt gctactaccc
     1501 ggcactgctc tacgagcgcg accccgatgt tcgagcgctc ctcctggcca gcatcgagcg
     1561 gagttggaga atcgagcagc ccgaacggaa tccgttcttc aacttcgtct acggagcggt
     1621 gaccggcaag ccttgcgacg tcgaggcggc cgaacgcacc ctccgcgagt accccatgga
     1681 gattcgcgac tggcgaatgg agaacagcca tcgggccgac atcatggtcg acccccggtc
     1741 aggccgattc gatgagaccc agagcacgcg ggtcctgccg tattcggaac ggtgtttcct
     1801 caaatggaac gggaacccgt accgccttga ctcgggcggc gacggcttga gcgaggacga
     1861 tggcgcggcc ttcctgttgc catactggat gggccgctac tacgggttcg tgacggaata
     1921 ggcggcgggg ccggtgatcc attctgatcg cgtccgtcac ctgaaccgcg ctgctccacg
     1981 ccgggggcgg tacgtggcct actggatgca ggcctcccag cgcgcccagt gcaaccatgc
     2041 gctggagtat gcggt
//
LOCUS       DAOSPL010000930         2055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_54488_length_2055_cov_2.728000, whole genome shotgun sequence.
ACCESSION   DAOSPL010000930 DAOSPL010000000
VERSION     DAOSPL010000930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54488_length_2055_cov_2.728000"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2044
                     /locus_tag="PLQ54_15325"
     CDS             <1..2044
                     /locus_tag="PLQ54_15325"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOS94690.1"
                     /translation="DVTRNLHRGRNALGVMLGNGFYNQHARDAWDFDRAPWRAQPALR
                     MQLRLEFADGRVRTIGTDETWRAHTGPVVLDGVRNGEFYDARREWPGWDRADFDDRQW
                     PAAEVVDGPKGVLRAQMMPPVRVMETLAPIRVTEPQPGVFVFDLGQTIAGWAQIRVTG
                     PEGTAIVLGYGEQLAADGTLDRGAIATLVYQGPFQTDSYTLAGRGVETWEPRFTYHGF
                     RYVEVTGWPGRPDLDSLRGRVVHTGFSRAGSFECSNALLNTIQRLTLWAYRGNFVGIP
                     TDCPHREKNGWTGDAQLACELGLLNFDNAPAYEKWMGDFRDEQPASGEFPGIVPTSGW
                     GYGVGPAWDSAHVLIPWYLYVYQGDRRVLEDNYASMKRYVDFLGTRARDHMVDYGLAD
                     WCPARTETPASVTSTAYYYVDALTLSRVAELLGQPDDAQRYAELAARIFDAFNAAHNR
                     GNGLYANGSQTALACALYQGLVPADQREWVAQRLVEAVEATDGHLDCGILGAKYLFRA
                     LTDAGRADVAYRVATQTTPPSYGDWVRRGATTLWEDWGGNASRDHIMFGDISAWFYSA
                     LAGINPDPRRPGFRHTIIRPHPVGDLTWVRAEHQSPYGPVGSAWELAAGGLTLRVTVP
                     PNATATVYVPAARPNAVTESGRPAAQSPGVAYLGPRDGCAVFAVGSGEYEFTASDS"
BASE COUNT          304 a          729 c          733 g          289 t
ORIGIN      
        1 cgacgtgacc cgcaacctgc atcgcggccg gaacgccctg ggcgtcatgc tcggcaacgg
       61 gttctacaac cagcacgcgc gcgatgcgtg ggacttcgac cgggccccct ggcgggcgca
      121 accggccttg cggatgcagc tccgcctcga gttcgccgac ggccgcgtgc gcaccatcgg
      181 caccgatgag acctggcggg cccacaccgg accggtggtg ctcgatggcg tccgcaatgg
      241 cgagttctac gatgcccgcc gcgagtggcc cggatgggac cgcgccgact tcgacgatcg
      301 ccagtggccg gcggcggagg tcgtggacgg gcccaagggc gtcctgaggg cgcagatgat
      361 gccgccggtt cgcgtgatgg agaccctcgc gcccatccgc gtgacggagc cccagcccgg
      421 cgtgttcgtg ttcgacctgg gccagaccat cgccggctgg gcacagatcc gcgtcacggg
      481 gcccgaaggg acggccatcg tccttggcta tggtgagcaa ctggcggccg acggcaccct
      541 cgatcggggt gccatcgcca cactggtcta tcagggtccg ttccagaccg acagctacac
      601 gctggccggc cgcggggtgg agacgtggga gccccgcttc acctaccacg gctttcgcta
      661 tgtggaggtc acgggctggc cgggccggcc ggatctcgac agcctccgcg ggcgcgtggt
      721 gcacaccggc ttctcgcgag cggggagctt cgagtgctcg aacgcgctgc tgaacaccat
      781 ccagcgcctg acgctgtggg cctatcgcgg gaacttcgtc ggcatcccca ccgactgccc
      841 gcaccgggag aagaacggct ggacgggcga cgcccagctc gcgtgcgagc tcgggttgct
      901 caacttcgac aacgccccgg cctacgagaa gtggatgggc gacttccgcg acgaacagcc
      961 ggccagtggc gagttccccg gcatcgtccc cacctcgggc tggggctacg gcgtgggccc
     1021 ggcatgggat agcgcccacg tgctgatccc ctggtacctc tacgtctacc agggcgaccg
     1081 gcgcgtgctc gaggacaact atgcgagcat gaagcgctac gtcgacttcc tgggcacgcg
     1141 cgccagggac cacatggtcg actacggtct ggcggactgg tgcccggcga ggacggagac
     1201 gccggcctcg gtgacctcga cggcctacta ctacgtcgac gccctgacac tgtcgcgcgt
     1261 ggctgagctg ctgggccagc ccgatgacgc ccagcgctac gcggagctgg ccgcccgcat
     1321 cttcgacgcc ttcaatgcgg cccacaaccg cgggaacggc ctctacgcca acgggagcca
     1381 gacggcgctg gcgtgcgcgc tctaccaggg cctggtgccc gcggaccagc gcgagtgggt
     1441 ggcgcagcgc ctggtcgagg cggtggaggc caccgatggg cacctcgact gcggcatcct
     1501 gggcgccaag tacctgttcc gcgccctcac cgacgccggc cgggcggatg tggcctaccg
     1561 cgttgccaca cagacgacgc cgccgtcata cggcgactgg gttcgccgcg gcgcgacgac
     1621 gctgtgggaa gactggggcg gcaacgcctc gcgcgaccac atcatgttcg gcgacatcag
     1681 cgcatggttc tatagcgcgc tggcgggcat caacccggat ccgcggcgcc cgggcttccg
     1741 gcacacgatc atccgtcccc accccgtggg cgatctcacc tgggtgcggg ccgagcacca
     1801 gagcccctac ggcccggtgg gcagtgcgtg ggagctcgct gccggcggac tgaccctgag
     1861 ggtgacggtc ccgccgaacg cgacggccac agtctacgtg ccggccgccc ggcccaacgc
     1921 ggtcaccgag agcggccgcc cggcggcgca gagcccgggt gtggcgtacc tcggcccgcg
     1981 cgacggctgc gccgtgttcg cggtgggctc gggggagtac gagttcacgg ccagcgattc
     2041 gtaggcgggg gccgc
//
LOCUS       DAOSPL010000931         2054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_54519_length_2054_cov_3.287144, whole genome shotgun sequence.
ACCESSION   DAOSPL010000931 DAOSPL010000000
VERSION     DAOSPL010000931.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54519_length_2054_cov_3.287144"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..>2054)
                     /locus_tag="PLQ54_15330"
     CDS             complement(31..>2054)
                     /locus_tag="PLQ54_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94691.1"
                     /translation="FRFGDCALDPATYPDGLASMRRVTDRLHAAGLCAGMQPYAFFID
                     KRSSWVTPKPDPRLAKSETYTLAEDLTADAVTVPVLETTASVSTVTGFFVHNSVIVQI
                     DDELIIYTGATKQAPYAFTGCQRGALGTTAAPHRRGATVGHLKECFGLLVPDPETTLF
                     EEVAAKTAELFNEGGFDCIYLDALDGEGILGGNDNAWHYGSKYVFEIWKRLKRPAVME
                     MSTFHHHLWFVRSRMGAWDHPTRSHKRFIDMHVEGNRTNQRMFLPSNLGWWAFMAWQG
                     SQTEPTYSDDIEYLCCKALGTDSGLSMTTFDPAQPAQARLAAIVRRYETLRHAGTVPE
                     AIRARLRQPGQDYSLVDGPGGAPAFVPMRYARHRVLGDEVWATQWTVRNEFAEQPAGL
                     RIEALSGAAPYEEPDAVALAGFDVPDEFPERAAAAGVSLDLLATRGPNGEAAGLIHAS
                     SSLGDPRGSWARLGKRFDPPLKLGDRQALGVWVRGDGKGEVLNFQLMSPEHLTSGIGE
                     HYVIVDFEGWRYFELIEPDSDLYADLAWPYGGSYSVYRESVTFDAIDRFSLWINHLPP
                     GEPVECAVAPVRALPLRTVSLQSPAVTIGGHTIGFPVTIETGSYLESLPGGPARVYDP
                     QGRLVAEVAPEGALPALGAGDNAVGFSCGRGKVAPRARVTVISRGEPLR"
BASE COUNT          337 a          679 c          703 g          335 t
ORIGIN      
        1 catgcgtggt tcccttctgg atgcccgatg ctaacgtagc ggctccccgc ggctgatgac
       61 ggtcaccctc gcccgcggcg cgaccttgcc tcgtccgcag gagaagccca cagcattatc
      121 cccggcgccg agcgcaggca gtgcaccctc gggagccacc tcggcgacga gcctgccctg
      181 cgggtcgtac acccgggccg gaccaccggg cagagactcg agataggagc ccgtctctat
      241 cgtcaccggg aacccgatgg tatggccgcc gatggtgacg gcagggctct gcaggctcac
      301 cgtccgcagc ggcagtgccc gaacgggggc gacggcacac tcgaccggct ctcctggggg
      361 caggtggttg atccacaggc tgaaccggtc gatggcgtcg aaggtcaccg actcgcggta
      421 gacggagtag gacccgccat agggccaagc gagatcggca tagaggtcgg agtccggctc
      481 gatgagctcg aagtaccgcc agccctcgaa gtccacgatc acgtagtgct cgccgatgcc
      541 gctggtcagg tgctctgggc tcatgagctg gaagttgagc acctcaccct tgccgtcgcc
      601 gcgcacccag acaccgagcg cttgccggtc gccgagcttc agcggggggt cgaagcgctt
      661 cccaagcctg gcccacgacc cgcgcggatc accgagcgag ctgctggcgt ggatcaggcc
      721 cgcagcctca ccgttggggc cccgggtcgc caataggtcg aggctgaccc cagctgcggc
      781 cgcccgttcc gggaactcgt cgggcacgtc gaaacccgcc agtgccacgg catccggctc
      841 ctcgtagggt gcagcgcccg acagcgcctc gatccgcaga ccggcgggct gttcggcgaa
      901 ctcgttccgg accgtccact gcgtggccca gacctcgtct ccgagtacac ggtgcctggc
      961 gtagcgcatg gggacgaaag ccggggcgcc gccgggtccg tccaccaggc tgtagtcctg
     1021 ccccggctgc cggagtcggg cccggatggc ctcgggaacc gttccagcat gccgcagcgt
     1081 ctcatagcgc cggacgatcg cagcgagacg tgcctgggcc ggctgggcgg ggtcgaaggt
     1141 ggtcatcgag agtccgctat cggtaccgag ggctttgcag cagaggtact cgatgtcgtc
     1201 ggagtaggtc ggctcggtct gtgagccttg ccacgccatg aaggcccacc aacccaggtt
     1261 gctggggaga aacatgcgct ggttggtccg gtttccctcg acgtgcatgt cgatgaagcg
     1321 cttgtgggag cgcgtcgggt gatcccaggc gcccatgcgc gaccgcacga accacaagtg
     1381 atggtggaag gtgctcatct ccatgaccgc cgggcgcttg agccgcttcc agatctcgaa
     1441 cacatacttg gacccgtagt gccacgcgtt gtcgttgccc ccgaggatgc cttcgccgtc
     1501 gagtgcatcg aggtagatgc agtcaaagcc gccctcgttg aacagctctg cggtcttggc
     1561 ggccacctct tcgaacagcg tggtctcggg atccggcacg agcaagccga agcactcctt
     1621 gaggtggcca accgtggcgc cgcgccggtg tggtgcggcc gtcgtaccta gcgcgccccg
     1681 ctggcagccg gtgaaggcat agggcgcctg cttcgtggca cccgtgtaga tgatcagctc
     1741 atcatcgatc tgcacgatga cgctgttgtg cacgaagaag ccggtgacgg tcgacaccga
     1801 agcggtggtc tcgaggaccg gcacggtcac ggcgtcggcc gtgaggtcct cggccagcgt
     1861 gtaggtctcg ctcttggcca gcctcgggtc gggctttggg gtgacccacg aagagcgctt
     1921 gtcgatgaag aacgcataag gctgcatccc ggcgcagagc ccggcagcat ggaggcggtc
     1981 ggtcacccgc cgcatgctgg ccagtccatc gggataggtg gccgggtcca gcgcgcagtc
     2041 accgaatcgg aacg
//
LOCUS       DAOSPL010000932         2054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_54520_length_2054_cov_3.229115, whole genome shotgun sequence.
ACCESSION   DAOSPL010000932 DAOSPL010000000
VERSION     DAOSPL010000932.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54520_length_2054_cov_3.229115"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="PLQ54_15335"
     CDS             complement(<1..1135)
                     /locus_tag="PLQ54_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94692.1"
                     /translation="MKGVGAMVGWAAWVTMVMWFLAPGAMGAEGEPATRSRHVPVIAT
                     GWDSPDTKQFREGLAAFEQWGVFDGTTLRASRRLATGEQKAATYAFANEKWEWSELAD
                     SLADLKAAQPATCLENYLMLYSNPGDVDWFDDDGWREVVDHWRLLARLAHEGGLRGLL
                     FDAEPYTPPYSQFLYRAQAGRDAHTFAEYRTQARQRGREVMEAVAAEFPEVTIFSYRL
                     FSDLLGLLDSGDLTRALEPDTYGLLPAFVDGWFDVAPSTVRVIEGTEDIGYRANSRAE
                     YDGAFTRLKLHLADFLDPEHREKVDRQFRVGHSLYLDAYTNPPDNPWHIDHTGSTAAA
                     RLAANLASALGAADGLVWLYGEKARWWRAGHADSPFWPDAFPGA"
     gene            complement(1210..1494)
                     /locus_tag="PLQ54_15340"
     CDS             complement(1210..1494)
                     /locus_tag="PLQ54_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94693.1"
                     /translation="MSQAVWVEAVVELAADRERRAADAAGQLAAQLEALACIREAELV
                     AADTAARNQLNADMLLAAAGAEAAWRELVSIMRAEWASIGRPLEPNDVAP"
     gene            complement(1517..1759)
                     /locus_tag="PLQ54_15345"
     CDS             complement(1517..1759)
                     /locus_tag="PLQ54_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94694.1"
                     /translation="MRRQPLAVQRGAGIRTHCYANDSEHCVTLSTIHPAASRNHPVAV
                     TADGRAIHSVSWHWHRIIEATDEELRAFEAAGYPAR"
BASE COUNT          347 a          702 c          671 g          334 t
ORIGIN      
        1 tagcgcctgg gaaagcatcg ggccagaagg gggagtccgc atggcccgcc ctccaccatc
       61 gggccttctc gccatagagc cagaccaggc catcggccgc accaagcgca gaagcgaggt
      121 tagcggccag acgcgccgcc gcggtgctgc ccgtgtggtc gatgtgccac ggattatcgg
      181 gagggttggt atatgcgtcg agatagagac tgtggccgac gcggaactgc cggtcgacct
      241 tctcccggtg ctccggatcg aggaagtcgg ccagatgcag cttcagtcgc gtgaatgcgc
      301 catcgtactc ggctcgcgag ttcgcgcggt agccgatgtc ctcggtgccc tcgatgactc
      361 gaaccgtcga tggcgccaca tcgaaccagc catcgacgaa tgccggaagc aggccatagg
      421 tgtcgggctc gagcgcccgg gtcaggtcgc cactgtcgag cagccccaag aggtcggaga
      481 agaggcggta actgaagatc gtcacctcgg ggaactcggc ggcgacggct tccatgacct
      541 ctcggccgcg ctgccgggcc tgggtacggt actcagcgaa tgtgtgggca tcgcggccgg
      601 cctgtgcccg gtagaggaac tgcgaatacg gcggcgtgta cggctcggca tcgaacagca
      661 gccctcgcag gccgccctca tgagccaggc gggcaagcag gcgccagtgg tcgacaacct
      721 cccgccaccc gtcgtcgtcg aaccagtcca catcacccgg gttggagtag agcatcaggt
      781 agttctcgag gcatgtggcc ggctgggcgg ccttcaggtc ggccagggag tccgcgagct
      841 cgctccactc ccacttctcg ttggcaaatg cgtaggtggc ggccttctgc tcgccggtcg
      901 ccaggcgccg ggacgcgcgg agggtcgtcc cgtcgaacac gccccactgc tcgaaggcgg
      961 ccaggccctc gcggaactgc ttcgtgtcgg gcgaatccca gccggtggcg atgaccggca
     1021 cgtgccggga gcgcgtggcg ggctcccctt cggcccccat cgccccgggc gccagaaacc
     1081 acatgaccat cgtcacccat gcggcccacc cgaccatggc gccaaccccc ttcacgaagc
     1141 aggactccct tcgtgggacg gcgcgggtgt ctcctgcaat ccgagagcgc tcagagccac
     1201 gccagaggac tacggcgcca cgtcattggg ttcgagtggc cgcccgatac tcgcccactc
     1261 cgcgcgcatg atgctgacga gctctcgcca cgccgcctcg gctccggccg cggccaacaa
     1321 catgtcggca ttgagctggt tccgcgcggc cgtgtcggcc gcgacgagtt cggcttcccg
     1381 gatgcacgcg agcgcctcaa gctgcgcggc aagctgccct gccgcgtcgg cggcccgacg
     1441 ttcgcggtct gccgccagct caaccaccgc ctcgacccac acggcttggc tcatcgcaat
     1501 acctccgtcg ctccgctcac ctcgccggat acccggcggc ctcaaatgcc ctgagttcct
     1561 cgtcggtcgc ctcgatgatg cggtgccagt gccaggagac ggaatggatg gctcgaccgt
     1621 cggcggtcac cgccacggga tggttcctgc tggcggcggg atggatggtc gagagagtca
     1681 cacagtgctc ggagtcattg gcgtagcagt gggttcttat gcccgctcct ctctgaacag
     1741 ccagtggttg gcgcctcaca ctctcccgcc accgccagcg cccgccaaag agccggtgga
     1801 atcgcgtcac gtcagtccgg aggtatgtgg gcagtacccg ccaggcccgg attggcatgc
     1861 accacaagca gtgttcctgg caagctcgat cttcgttcac aggtttccct gcgatgaaag
     1921 ggagatcaac cacattgccg agcaaacgct ttcagacagc gcacatcatg acccgtgcaa
     1981 tcgttgtggc cccatgtgag cgcacatcca gggggacggt ggcgaggcag ggcccagtct
     2041 cgcggcagtc gtcg
//
LOCUS       DAOSPL010000933         2046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_54810_length_2046_cov_2.631341, whole genome shotgun sequence.
ACCESSION   DAOSPL010000933 DAOSPL010000000
VERSION     DAOSPL010000933.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54810_length_2046_cov_2.631341"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PLQ54_15350"
     CDS             <1..485
                     /locus_tag="PLQ54_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS94695.1"
                     /translation="FAANTVVIVLGQLVALRFIPGRSRTRVLALAASIWSVSWLVVAM
                     TGRVEGGAAVVFAILGLGLFGVGETLWAPVAPAVVNDLAPEHLRGRYNSAQAMVWTTA
                     SVLGPATAGLLLGNGLAGVWVTATVGGTALAAALFLRLRRHLSPSADGRVAVSGTAGE
                     "
     gene            1323..1679
                     /locus_tag="PLQ54_15355"
     CDS             1323..1679
                     /locus_tag="PLQ54_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOS94696.1"
                     /translation="MPHVTTDPVRIPVPGGKVINEHVGQVATSTSAVSVAHMLAPSGW
                     EEPFQTPEFDEITVVVRGSLHVDCDGQRYEVSAGQSIITKAGERVQYSAGPLGAEYVA
                     ICLPAYTPETAHREGS"
     gene            1676..>2046
                     /locus_tag="PLQ54_15360"
     CDS             1676..>2046
                     /locus_tag="PLQ54_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005207855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOS94697.1"
                     /translation="MSTSTRPDAATVLAAPKVLLHDHLDGGVRSQTILEIADEIGYAD
                     LPAHDADSLGTWFRESADSGSLERYLETFRHTVAVMQTEAGLRRVARECVLDLAADGV
                     VYAESRYAPEQHLAGGLRLEQV"
BASE COUNT          313 a          678 c          725 g          330 t
ORIGIN      
        1 ccttcgcggc caacaccgtg gtcatcgtgc tcggccagct cgtcgccctg cgcttcatcc
       61 cgggccgcag ccgcactcgc gtgctcgcct tggccgccag catctggtcg gtgtcctggc
      121 tcgtcgtcgc gatgaccggg cgagtcgagg gcggcgccgc ggtggtcttc gccatcctcg
      181 gcttgggcct gttcggtgtc ggcgagacgt tgtgggcgcc ggtcgcgccg gccgtggtca
      241 acgacctcgc ccccgaacac ctgcgcggcc gctacaactc ggctcaggcc atggtgtgga
      301 cgacagcgtc ggtcctcggg cctgccactg ccgggctgct gctgggcaac ggcctggcgg
      361 gcgtctgggt gaccgccacg gtcgggggca cggcgctggc cgctgccctg ttccttcggc
      421 tacgccgcca cctgagccca tcggccgacg gccgggtggc tgtgagcggg actgcgggcg
      481 agtgaccgcg ggctgtgtgg ggctgagggg aagcccacga gcccgcggcc actccgacga
      541 ggccgcccaa tgtgggaggg aggcggttcc cgccgcccgc cgacgcgcag ccgcgtcgag
      601 gggaacgaga aatcggggcg agcatgccac ccacatgcgc gttgagtcac gctgacggtt
      661 aatccagatc aggagttaaa gcaaacctgt gatgattctt gatcgaaatt aatccccgtc
      721 gtgacattcg ctttgcgctc tgccagcatg acggagcgcc agcccccggg ctgatcatcc
      781 gagagtggcg tggcacatca gaagtctcct cgggcggtcc gccgtgcggg gaggagccgg
      841 gatccggggg gattccgtct cggaccgcac cacacggtgc ggatcgtcac cctcggggag
      901 caacgggttg agggaccggt tgtcactcag agcggccacc gagactcccg gttgaccgtg
      961 gactcgtcgg gttgatgcgg cccgtcggga ccaacagggg ccccacgcag atgttctgcg
     1021 tgggcctgca ggggaaagtc agctcagtca tatgggacac ctacggtcca cgatcgcgaa
     1081 aggcgggcgg tatgcgcggg aaatgcatcc gtccgaacaa ccgcgacttg gggagcggtt
     1141 cctgcggcga cttgtgcaac gcgtagctgt tcatgagtgg ctggccggta cacccggcga
     1201 acccacggcg atgggacttg ggcagtcctg tcgccgtggg actgccgggc gtgggattgc
     1261 cggtcgtggg attggcggtc gcaaggctga cggccgcgac ggtggatgac tagagttcga
     1321 ggatgccgca cgtcaccacc gacccagtcc ggatcccggt ccccggaggc aaggtcatca
     1381 acgagcacgt cgggcaggtg gcgacctcca cctccgccgt atcggtcgct cacatgctcg
     1441 cgccctccgg ctgggaggaa ccctttcaga cgccagagtt cgacgagatc accgtcgtcg
     1501 tccggggctc gctccatgtc gattgcgacg gccagcggta cgaggtgagc gcgggacagt
     1561 cgatcatcac gaaagccggc gagcgcgtgc agtactccgc cggcccgctc ggcgccgaat
     1621 acgtcgctat ttgcctgccg gcatacacac cggaaactgc ccatcgcgag gggtcatgag
     1681 cacgagcacc cggccggatg cggccaccgt cctggccgcg ccgaaggtgc tcttgcacga
     1741 ccacctcgac ggcggcgtcc ggtcgcagac catcctcgag atcgccgacg agatcgggta
     1801 tgccgacctg ccggcccacg acgccgacag cctcggcacg tggttccgcg agtcggccga
     1861 ctccgggtcc ctggagcgct atctggagac cttccggcat accgtggcgg tcatgcagac
     1921 cgaggccggg ctgcggcggg tcgctcgcga gtgcgtcctg gacctggccg cggacggagt
     1981 cgtctatgcc gagagccgct atgcccctga gcagcatctc gccggcgggt tgaggctgga
     2041 acaggt
//
LOCUS       DAOSPL010000934         2045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_54831_length_2045_cov_3.849749, whole genome shotgun sequence.
ACCESSION   DAOSPL010000934 DAOSPL010000000
VERSION     DAOSPL010000934.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54831_length_2045_cov_3.849749"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..889
                     /locus_tag="PLQ54_15365"
     CDS             236..889
                     /locus_tag="PLQ54_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOS94698.1"
                     /translation="MKRRAGFTLIELLVVIAIIAILAAILFPVFAKAREKARQASCTS
                     QLKELGLAVLMYAGDYDDRLVDHCGQTTANCWAVSLFPYVKNAEVWRCPSYGGTDTFG
                     IWRDYANGPSHDFSPRLPRSYAWNLMIDNRLMAEIDKPAVVPCVADGDGTGWMAPMHS
                     CIGFTDFGASASCATKYARMSPRHNEGAVLAMLDGHAKWFKGSQIISDFAAGNLLPQ"
     gene            complement(1357..>2045)
                     /locus_tag="PLQ54_15370"
     CDS             complement(1357..>2045)
                     /locus_tag="PLQ54_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94699.1"
                     /translation="SRTSTDELGQAESAVASAFSRVADKRVCASALTAAMARAEVAPR
                     CALIRLTGAAPTDEALGAVRAALRDGQAEVRAAAFRVLCDWATPAAAPDLLELARGGP
                     DTERTAALRGYMRLIGSPDLSPEQKMAMCNEASALCRTTEDIDALLSGLATVASLDAL
                     NMAMSHLDNAPLTNKVSFAAVCIGELLEQKHPAEVAAAMERVLKAMNNPGMERRVRSC
                     MERAQKAAGQ"
BASE COUNT          352 a          750 c          622 g          321 t
ORIGIN      
        1 gcttcatcgg gtccatcctc ctcgtgggat gcaagacccg caccgactcg cccgagcaac
       61 cggacatcac cgacgggaac ttccccaaag cggagatgac caaggatcct tcccggatgc
      121 agaagggcat gggcaaagga cccggcgcgc ctgcgacgcg ctaggatcct gctctctctg
      181 aaccatgtgg aacgccatgc ggcggcgtgc tgaggagtgt gaaggagggc ttttcgtgaa
      241 acgacgtgcg gggttcaccc tgattgaact gctcgtggtc attgccatca tcgccatcct
      301 ggcggcgatc ctgtttcccg tgttcgccaa ggccagagag aaagcccggc aggcgtcgtg
      361 caccagccag ctcaaggaat tgggtctggc cgtgttgatg tacgccgggg actacgacga
      421 ccgactggtc gaccactgcg gccagactac ggccaactgc tgggccgtct ctctcttccc
      481 ctatgtgaag aacgccgaag tatggcgctg cccgagctac ggcggcacgg acacgttcgg
      541 tatctggcgc gactacgcca acgggccgag ccatgacttc agtccgcggc tcccccgaag
      601 ctacgcctgg aacctcatga tcgacaaccg actcatggcc gagatcgaca agccggccgt
      661 ggtgccctgt gtggccgacg gcgatggcac cggctggatg gccccgatgc acagctgcat
      721 cgggttcacc gacttcggtg ccagcgccag ctgcgctacg aagtacgccc gcatgagccc
      781 gcggcataac gaaggcgccg tgctcgcgat gcttgatgga cacgccaagt ggttcaaggg
      841 ctcgcagatc atcagcgact tcgcggccgg caacctgctg ccccagtaag cccacctgac
      901 gccatcccga acgcaccgga ggccgtggcc tggtcaagcc acggcctccg gtcactgtgg
      961 agccaacacc cggcgccacc gcccgacggc tgcgcttgcc tccacaacca acgcgcaccg
     1021 ccccagccaa cctcgacagc tctcgccacc gatccctgcc tctcctccgc accccagcac
     1081 ccgacgcaga cccccccaat cggataccca acctgacacc catgctgcct gcatgatccc
     1141 gactaggagg ccacccatgg acgagtatcg gctccgagtg tggagatacg atggagacgc
     1201 cgtggagttt cgctgcggaa tcggcccagc cgcggtggcc gcgcaccaca agcggcgggg
     1261 catgcaccca tccgccgcgc cgcctcgaag atagcagcgc accgcatgcc gggacgacac
     1321 acggtgcgct gaggggctcg agccgactcg gttcgcctac tggccggccg ccttctgcgc
     1381 ccgctccatg cacgagcgca cccggcgctc catgcccggg ttgttcatcg ccttgagcac
     1441 gcgctccatg gccgcggcga cctcggccgg atgcttctgc tccagcagtt cgccgatgca
     1501 caccgcggcg aagctcacct tgttcgtcag tggggcgttg tccaggtgcg acatggccat
     1561 gttcagcgca tccagcgacg ccacagtagc caggccactg aggagcgcat caatatcctc
     1621 ggtggtccgg cacagcgccg acgcctcatt gcacatggcc atcttctgct cgggcgacag
     1681 gtcgggcgat ccgatcagcc gcatgtagcc ccgaagcgcc gccgtccgtt cggtgtccgg
     1741 tccgccccgg gccaactcca gcaggtccgg cgccgccgcc ggcgtggccc agtcacacag
     1801 aacccggaac gctgccgccc gcacctcggc ctgtccgtcc ctcagagcgg ctctcaccgc
     1861 gcccagtgcc tcgtctgttg gggcggcgcc ggtgaggcgg atcaacgcgc acctgggtgc
     1921 gacctcggct ctcgccatgg ccgccgtcag agcactcgcg cagacacgct tgtccgccac
     1981 gcggctgaag gcgctcgcca cggcgctctc cgcttgcccc agctcgtccg tgctggtgcg
     2041 cgaca
//
LOCUS       DAOSPL010000935         2045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_54851_length_2045_cov_2.605528, whole genome shotgun sequence.
ACCESSION   DAOSPL010000935 DAOSPL010000000
VERSION     DAOSPL010000935.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54851_length_2045_cov_2.605528"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="PLQ54_15375"
     CDS             <1..1001
                     /locus_tag="PLQ54_15375"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOS94700.1"
                     /translation="GLPVALAPEEDPTVVRQVVAAMTPQPSEAWVASLRERLTQLTQR
                     DPAEREPTLPPGWVPVVRSARDPGPSPAVPRPADSPPPVIESPGPKPVVPIVPRHLKS
                     VALIFDDGPHPLVTPALLRILAEQDVKASFFLVGTQAEQYPELVRRIAQEGHDVGNHT
                     YEHLNLDGLLAPEVQRQMLGGKALLEAILGRPVTYFRAPGENFGPRAQLVASQANLTV
                     IPSSLNTWPIMSLAPEEIARRMASQAQDGSTILLHNGQDKTVYCLEPLIRMLRDAGFE
                     FLTVGQWLQRYPGRAFEAPATPLTPVAGATTPTTGVAAAPSSSAVDELAVPRAGAH"
     gene            1127..>2045
                     /locus_tag="PLQ54_15380"
     CDS             1127..>2045
                     /locus_tag="PLQ54_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94701.1"
                     /translation="MAFEPGVTSTSLTPWPCSSLFDFSFRLEAPTGKHGFLTVDPEGR
                     FRFTDGTPARFWGVNVARDGVFAQREVIDRVVATFARSGVNLVRFHHLDGPGGIVGQG
                     GGLNADRVAALDYWIARLGQRGIYVYLDLLDYRVFSEPTVERGVDLGRGAKPYAVFVP
                     ELIALQKDYAERLLREHVNPHTSLAYADDPTVAFVELYDENGLFIRRNDIPTLREPYR
                     ERLKQRWNEWLRARYSDSDRLRREWSTGGSGSDLLPFELLEKGTVALPILAPGSGGTT
                     ATGARQGPRRRADGARFFHEVHVDYFSQMR"
BASE COUNT          318 a          735 c          672 g          320 t
ORIGIN      
        1 gcggtctgcc cgtagcgctg gcccccgaag aggatcccac agtcgtccgc caagtcgtgg
       61 ccgccatgac gccccagccg tcggaggcat gggtcgcatc cctgcgcgag cggctcaccc
      121 agttgaccca gcgcgacccc gcggagcgtg agcccactct gcccccgggt tgggtccccg
      181 tagtccggag tgcgcgagat ccgggcccca gccccgcagt gccccggccg gccgactccc
      241 cgccccccgt gatcgagagt ccggggccca aaccggtggt cccgattgtg cctcgccacc
      301 tgaagtccgt ggccctgatc ttcgacgatg ggccccaccc cctcgtcacg cccgcgctgc
      361 tgcggatcct ggccgagcaa gacgtgaagg ccagcttctt cctcgttggc acccaggccg
      421 agcagtaccc cgagctggtg cgccgcatcg ctcaggaggg ccacgacgtg gggaaccaca
      481 cctatgagca cctcaacctc gatgggctct tggcgccgga ggtccagcgg cagatgcttg
      541 gtggcaaggc tctcctggag gccatactcg gccgaccggt gacgtacttc cgcgcgccgg
      601 gcgagaactt tggaccacga gcgcaactgg tcgcgagcca ggcaaacctg acggtcatcc
      661 ccagcagcct gaacacctgg cccatcatgt cgctggcgcc cgaggagatt gcgcgccgca
      721 tggcaagcca ggcgcaggac ggctcaacga tcctgctgca caacgggcag gacaagaccg
      781 tctactgcct cgagccactc atccggatgc tgcgtgatgc cggcttcgag ttcctcaccg
      841 tgggacagtg gctccagcgg taccccgggc gtgccttcga ggcacccgcc actccgctaa
      901 cgcccgtcgc gggggcgacg acgcccacga ccggggttgc tgcggcaccg agcagttccg
      961 ccgtggacga gctcgcggtg ccccgggcgg gagcccactg agagaccccc acgtcgcgtg
     1021 ccccctcggc cacgcgtggg tgtggtgcgc gctggcggcg gggtggtctg cagccgcatt
     1081 aggtacggcg gccggccaac aaccgggctc gccgccctcc tggccagtgg cgttcgaacc
     1141 gggggtcacg agcacctcgc tcactccgtg gccgtgctcc agcctcttcg acttcagttt
     1201 ccgcctcgag gcacccaccg gcaagcacgg cttcctcacc gttgatccgg agggacgctt
     1261 ccgtttcacc gacgggacac ccgcgcgttt ctggggcgtc aatgtggcgc gcgacggcgt
     1321 tttcgcccaa cgtgaggtca ttgatcgcgt cgtggcaacc ttcgcacggt ccggcgtgaa
     1381 cctggtccgc ttccaccacc tcgacgggcc gggaggcatc gtcggccagg gcggcggcct
     1441 caacgccgat cgcgtggcgg ccctggacta ctggatcgcc cggctcggcc aacgggggat
     1501 ctatgtctat ctcgacctcc tcgactaccg cgtgttcagc gaacccacgg tcgaaagggg
     1561 ggtggacctg ggtcgtggcg cgaagccgta tgccgtgttc gtgcccgagc tcatcgccct
     1621 gcagaaggac tatgccgaga ggctgttgcg ggaacacgtg aacccacaca cgagcctggc
     1681 ctatgccgat gacccgaccg tggccttcgt cgagctctac gacgagaacg gcctgttcat
     1741 ccgccgcaac gacattccga ccctccggga gccataccgg gagcggctca agcagcgatg
     1801 gaatgagtgg cttcgggccc gatactccga cagtgaccgg ctgcgccgcg aatggtcgac
     1861 tggagggagt ggcagcgacc tgctcccgtt cgagctgctc gagaaaggaa cggtggccct
     1921 gccgatcctg gcccctggga gtggcggaac gaccgcgaca ggtgcccgcc aggggccgcg
     1981 gcgccgggcc gatggcgccc gcttcttcca tgaggttcac gtcgactact tctcccagat
     2041 gcgcg
//
LOCUS       DAOSPL010000936         2042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_54964_length_2042_cov_2.822849, whole genome shotgun sequence.
ACCESSION   DAOSPL010000936 DAOSPL010000000
VERSION     DAOSPL010000936.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54964_length_2042_cov_2.822849"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PLQ54_15385"
     CDS             complement(<1..284)
                     /locus_tag="PLQ54_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HOS94702.1"
                     /translation="MRDRQSTLRRILDSGIVAVMRARSSEHLIGVADAIRAGGVDCIE
                     VTMTTPNALGIIEQTARTFGDDAVIGVGTVLDAESARAAILAGAQFIVAPT"
     gene            complement(351..983)
                     /locus_tag="PLQ54_15390"
     CDS             complement(351..983)
                     /locus_tag="PLQ54_15390"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOS94703.1"
                     /translation="MALAELIDISVAIHPRMHVWPGDPPVRVDRVASLADGDSCNLSR
                     LELCAHTGTHIDAPLHFVRGGAPVDQLPLDAFVGPARVVRVADGAAIGEDDIQASAVR
                     EGERILFRTANSERWAGPGFLTDFVAFTDEAARLLASLRPRLVGIDYLSVQPFHADTA
                     GVHEALLGAEIVVLEGLNLAGVQPGEYELICTPLLIAGCEGAPARALLRR"
     gene            complement(1016..1936)
                     /gene="argF"
                     /locus_tag="PLQ54_15395"
     CDS             complement(1016..1936)
                     /gene="argF"
                     /locus_tag="PLQ54_15395"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOS94704.1"
                     /translation="MKQDLLTILDLGKDLVEEILDLAVDMKTHRGAPAHRSILGGKSV
                     ATIYEKPSTRTKLSFAVGIYELGGNAIELTASGSQLSRGETYADTGHVLERYVHGVVF
                     RALKHEAVCELAAACGVPVINALSDQHHPCQILGDLMTVQERFGRLHGVEIAFVGNGS
                     NIVHSLMCGVAVLGLKLRVATPPSATPLDSVKELVAPVAQASGAVIEYGVDPIAMVTG
                     ADVVYTDVWFDMGKDHERDARLHEYRPYQLDDALLAHAKPTAVVMHCLPAMRGEEITN
                     EVMDGPQSIVFDQAENRLHVQKAIMAKLLV"
BASE COUNT          334 a          700 c          683 g          325 t
ORIGIN      
        1 gtgggtgcga cgatgaactg ggcgccggcc aagatggcgg ctcgggcgga ctctgcatcg
       61 agcaccgtac cgacgccgat gacggcgtcg tcaccaaatg tccgggcggt ctgctcgatg
      121 atgcccaagg cgttcggcgt cgtcatcgtg acctcgatgc agtcaactcc gcctgcccgg
      181 atcgcgtcgg ccacaccgat gaggtgctcg ctgctgcggg cgcgcatcac ggccacaatg
      241 cccgagtcca ggatgcgacg cagcgtgctt tgccggtctc gcatgggaac cgcctcctcg
      301 cgtcgaggcg gttcgccgag cgtcaggcgc cgccctccag ccgtggggcc ctaacggcgg
      361 agcagtgccc gggccggtgc gccctcgcac cccgcgatga gcagtggcgt gcagatcagc
      421 tcgtactcgc cgggctgcac gcccgccagg ttcagcccct ccagcaccac gatctccgcg
      481 ccaagcagag cctcgtgcac cccggcggtg tccgcgtgga atggctgaac ggagagatag
      541 tcaatgccga cgagccgggg gcgtagggac gccagcagtc gagcggcctc atcggtgaac
      601 gctacgaagt cggtgaggaa ccccgggccg gcccaacgct ccgagttcgc ggtgcggaac
      661 aggatgcgtt cgccttcccg tacggcggag gcttggatgt cgtcctcacc gatcgcggca
      721 ccatcggcga cgcggaccac gcgcgcgggc ccgacgaagg cgtcgagcgg aagctgatcc
      781 accggcgcgc cgccgcggac gaagtggagc ggcgcgtcga tgtgcgtgcc cgtgtgcgcg
      841 cacagctcca ggcgcgagag gttgcacgag tccccatcgg ccagcgaggc cactcggtcc
      901 acccgcactg gggggtcccc gggccatacg tgcatgcgcg gatggatcgc gaccgagatg
      961 tcgatcagct ccgccaaggc caccacccgc cctccgcgga tcagtccagc tcgagctaga
     1021 cgagcagctt agccatgatc gccttttgga cgtgcaggcg gttctcggcc tggtcgaaca
     1081 cgatggactg gggaccgtcc atcacctcgt tggtgatctc ctccccgcgc atggcgggca
     1141 gacagtgcat caccaccgcc gtcggcttcg cgtgggccag cagagcgtcg tcaagctggt
     1201 acggccggta ctcgtggagt cgggcgtccc gctcatggtc ctttcccatg tcgaaccaga
     1261 cgtccgtgta gaccacatcg gccccggtga ccatggcgat ggggtcgacc ccgtactcga
     1321 tgaccgcacc ggaagcctgt gcgaccgggg caaccaactc cttcaccgag tccagcggcg
     1381 tggccgaggg aggcgttgcc acccggagct tcagtcccag cacggcgacc ccgcacatca
     1441 gggagtgcac gatgttgctg ccgttgccaa cgaaggcgat ttcgacgccg tgcaggcgac
     1501 cgaatcgctc ctgaacggtc atgaggtcgc ccagaatctg gcacggatga tgctggtcgc
     1561 tcaacgcgtt gatgacgggc acgccgcacg cggcggccag ctcgcacacc gcctcgtgct
     1621 tcagtgcgcg gaacacgacg ccatggacgt accgctcgag cacgtgtccg gtgtcggcat
     1681 aggtctcccc ccggctgagc tggctccccg acgcggtgag ctcgatggcg ttcccgccca
     1741 gctcatagat cccaacagcg aacgacagct tggttcgcgt cgatggcttc tcgtagatgg
     1801 tcgccaccga cttcccgccg aggatcgacc gatgtgcggg cgccccacga tgtgtcttca
     1861 tgtcgaccgc caggtcgagg atctcctcaa cgaggtcctt gccgaggtcc agaatggtca
     1921 agaggtcctg cttcatcgcg cagtgcactc cttcagtgcg tctcgagagt tgagcgccgc
     1981 tcagggggcg aagacctggt aggccgcccg gcgccgcgcg tcgaaggtgg acgcgtcctc
     2041 gg
//
LOCUS       DAOSPL010000937         2040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_55038_length_2040_cov_3.069521, whole genome shotgun sequence.
ACCESSION   DAOSPL010000937 DAOSPL010000000
VERSION     DAOSPL010000937.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55038_length_2040_cov_3.069521"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="PLQ54_15400"
     CDS             complement(<1..719)
                     /locus_tag="PLQ54_15400"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOS94705.1"
                     /translation="MGDCQGAGGSSQPGLRRDLSLFTLVAIAVCTVIGGGINLLTVEV
                     QAKVPGIGSLVPLAFILGVAPALFSALCYAILGSAMPRAGGGYIYASRGLSPMLGFLA
                     AFSKWFGMASAIGVLAYIDVPLLRDTARLWGFEGIAQFLSTSWGTLWIPVATIVLFWF
                     TNLVGVKCLGTTVVVLMVAEFAGGLCLIATGLLNGPEAFAARMAQATPPVDVMALAGA
                     ATVTQGGLRELVALRAEGAHR"
     gene            1277..>2040
                     /locus_tag="PLQ54_15405"
     CDS             1277..>2040
                     /locus_tag="PLQ54_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94706.1"
                     /translation="MRGVPRVLACALVAAGPLMAAGPPTAVPTLDELRQIIAQQRGFA
                     ARLTAQITSVGAGPFVYPYPRVPEDYTVEVRRTSVAWGPGELIVDHAPWRTVKLAEGA
                     PDLDPLRPYRYCRARNGRMYVIEDLAITQPAQLQSWARATAIEQPEPDGVSVDTVESA
                     LQLSAGEPASLAGFVAPGLQGDGARITRETLDGLGCIRVDVPYYDPTGYISTWFAPER
                     GYLAVRRETALTHKGMRRTIVHHVREFAQLEGVGWFP"
BASE COUNT          343 a          731 c          687 g          279 t
ORIGIN      
        1 tcgcgatgcg ccccctccgc acgaagcgca acgagctccc gcagaccgcc ctgggtgaca
       61 gtggccgcgc ctgccagggc catcacatcc acgggtgggg tcgcctgggc catgcgcgcc
      121 gcgaaggctt ccggcccgtt cagcagcccg gtggcaataa ggcacagccc acccgcgaac
      181 tcggcgacca tcaaaacgac cacggtcgtg ccgaggcact tgacacccac caggtttgtg
      241 aaccagaaca gcacgatggt ggcgacgggg atccagagcg tcccccacga ggtcgacagg
      301 aactgcgcga tgccctcgaa gccccacagg cgtgccgtgt cgcgcagaag aggcacgtcg
      361 atgtaggcca gcacaccgat ggctgaggcc atgccgaacc acttcgagaa cgcggcgagg
      421 aacccgagca tcgggctgag accgcgagag gcgtatatat agcccccccc cgcccgcggc
      481 atggccgagc cgaggatggc gtaacagagc gcggagaaca gggcgggcgc gacgcccagg
      541 atgaatgcca gcggcaccag cgatccgatg cccggaacct tggcctgcac ctcgacggtc
      601 agcaggttga tcccgccgcc gatcaccgta cacactgcga tggccactaa tgtgaaaagc
      661 gataggtcac gcctcagtcc cggctgggat gatcccccgg cgccctgaca gtctcccata
      721 cccgtccgat ctccctcttg cccgcacatg ggggactaat gcggcgttcc cgccagcgcc
      781 gcgctcttcg tttcgccccg gaggcggacc tcccgcgcgg gagaggggca tcggtcccac
      841 gcgaatccgt cgggtggaac tgtcggctcc ctcgccagtg ctccggcgcg gcgaggtcct
      901 ggcagtcagc ggaacgacac ccgtggccgt cggcgccgat ggcgcacctg ggttgtgcgt
      961 cgcactcatg aatgaggatg gggtgtatac cggagccaga cgaagagggg gccgagcctt
     1021 ctgccaccag aagccgtgct gcatcgccgc cgccggcacg gacgacggtg ccacctcagg
     1081 gccgtgcctc ggcgggctcc ctcgctagcc acaagcccgg cctacgtgcc ggctcccgcg
     1141 cggcgatgcg gctcccaaca ccgcgccgtg tgccctgcgg tccacggccc acccaacccg
     1201 ctcgcgggta ggggcatcca tggtaaccaa gaggttcgcc ggcatgccca cggctggttg
     1261 cggggagagg atctcgatgc gcggagtccc gagggttctc gcgtgcgcgt tggtcgccgc
     1321 tggcccgctg atggcggccg gtccgccgac agccgtcccc acactcgacg agctgcgcca
     1381 gatcatcgcg cagcagcgcg gcttcgcagc ccgcctgacg gcccagatca cgagcgtcgg
     1441 ggccggcccg ttcgtctatc cctacccgag ggtgcccgaa gactacacgg tcgaggtgag
     1501 gcggacgagc gtcgcgtggg gaccgggcga gctcatcgtc gaccacgcgc cgtggcggac
     1561 ggtgaagctg gccgagggcg cgcccgatct cgatccactc cgtccgtacc ggtattgccg
     1621 ggccaggaac ggccggatgt acgtgatcga ggatctcgcc atcacgcagc cagcgcagct
     1681 ccagagctgg gcgcgcgcga ctgctatcga gcagcccgag ccggacggcg taagcgtcga
     1741 caccgttgag tcggccctgc agctttcggc cggggagccg gcgtcgctgg ccgggttcgt
     1801 ggccccgggc ctgcagggcg acggagcccg gatcacccgc gagacactcg acgggctcgg
     1861 gtgcatccgc gtcgatgtgc cctactacga ccccaccggc tacatcagca cctggttcgc
     1921 ccccgagcgg ggctatctgg ccgtacgtag agagaccgcc ctcacgcaca agggcatgcg
     1981 ccggacgatc gtccaccacg tccgcgagtt cgcccaactc gagggcgtcg ggtggttccc
//
LOCUS       DAOSPL010000938         2036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_55201_length_2036_cov_2.019687, whole genome shotgun sequence.
ACCESSION   DAOSPL010000938 DAOSPL010000000
VERSION     DAOSPL010000938.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55201_length_2036_cov_2.019687"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /locus_tag="PLQ54_15410"
     CDS             <1..1280
                     /locus_tag="PLQ54_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94707.1"
                     /translation="MRDWSQDEVAYAVALTREVARLCRTWYLEDGSARGFATAAGVGH
                     LVRGQGDVLAGQQEALSILRRQFNTSRPPEMDEAIDALERGLAEVGWAWTPARTGSIR
                     GLDGFITPADKFGDLLPSQIPVRLLRGRTRNAGRGGLLGPHTTREDADILRAHVPGGY
                     FLVYSEPDGAIYFASAATTAGLLDRSGKSRDRVRVERSDRLTVDALNRIGHAARADRE
                     RLAEVVAAPGLREPVVRLFRTLSSAHHVFFEFLATMAFVDPENLLIVHERWARGVGGD
                     YLQEVVSTMNEYEVAPPVELLKNFPAASLMLTVMRTVVEQLDQIEAALGTHDPAIADK
                     LGVPGLRASVAAAISRSYRSADGTPLSATTTDGEQAAWLVLDAGHYFMSVALVGLIVD
                     RLTHSIREVVLPFVDGSPRLAPGPLWITYRPSS"
BASE COUNT          366 a          685 c          630 g          355 t
ORIGIN      
        1 agatgcgcga ctggtcacag gacgaagtcg cctacgccgt cgccctcacg cgggaggtgg
       61 caaggctgtg tcggacgtgg tacctcgagg atggctccgc gaggggcttc gcaacggcag
      121 ccggtgttgg gcacctggtg aggggccagg gtgacgtgtt ggccgggcag caggaggcac
      181 tttccatcct gcgccgacag ttcaacacat cacgcccacc tgagatggac gaagccatcg
      241 atgcgctaga gcgaggtctc gccgaggtcg gctgggcctg gaccccggca cggacaggct
      301 caattcgggg actggacggt ttcataacac ccgcagacaa gttcggcgac ctgctcccat
      361 ctcagatccc cgttcgcctc cttcgaggac gcacgcgcaa cgctgggcgg ggaggacttc
      421 tcggaccgca caccacacgt gaagacgctg atatccttcg cgcccacgtg ccaggcggct
      481 acttcttggt gtactcagaa ccggacgggg cgatctactt cgcgtccgct gcgacgactg
      541 ccggtttgct agatcgcagc ggcaagagcc gcgaccgggt tcgagtggag cgctcggacc
      601 gtcttaccgt cgatgcgctc aacaggatcg gacacgctgc gagggctgac cgggaacgct
      661 tggccgaagt ggtggccgcg ccgggcctac gcgaacctgt ggtgcggctg ttccggaccc
      721 tcagctcggc ccaccatgtc ttcttcgagt tcctcgccac gatggctttc gtcgatccgg
      781 agaacctcct gattgtacat gagcgttggg ctcgcggcgt cggcggcgac tacctccaag
      841 aagtcgtttc cacgatgaac gagtacgagg tagcaccgcc cgtcgagctg ctgaagaact
      901 tccccgctgc atcactcatg ctgacggtaa tgcgcaccgt cgtcgagcag cttgaccaga
      961 tcgaggccgc cctcggaacg cacgaccccg caatcgccga caagctcggc gtgccgggcc
     1021 tgagggcctc cgtagcggcc gccatcagcc gctcgtatcg cagcgcggac ggcacaccgc
     1081 tgtcagcgac cacaacggat ggtgagcagg ccgcatggct cgtcttggac gcggggcact
     1141 acttcatgag cgtcgcgctc gtgggcctca tcgttgaccg cttgacgcac tccatacgag
     1201 aagtggtcct gccattcgtc gatggcagcc cacgactggc tccaggccca ctttggatca
     1261 cataccgccc atcctcgtga ctgcggtgga ttcgttgcgc agcaacggtt ttcgtgcact
     1321 atcccggctg ttgctttact ggcaccggac attattcgaa cccagtgaat cccacgcgtt
     1381 tcactacccc aactgggggg cgtcagccga acgcggttcc ttggagatgg ccgaccttct
     1441 cttccttggg tcccggctgt acagtcgaaa ggtccaactc aacagcgcac tgtcagacct
     1501 tcgccgcttc ggcatctgtg tatcccccgg gggtacacaa cccgtgtacg ccgtccttcg
     1561 aacaaggaga cgcgatgcac accgaggcac cgatgccggc cccccagcgt cccacccgct
     1621 gggacgttgt tcgggccgtg atcacaggag cagtctccgg actggtccgt tgggttttgg
     1681 acgcgacgtt gcgacacctc gcgtagcaac accgtcgagc tggacacccg ggccagcgca
     1741 atcctccggg acgcaccgca cggccggata gccgtagctc atcacctatt gacttgagtg
     1801 cactcagacc cctccacatc cggccgcgga tcgagatgtg gaggggtctt ctcatgcccg
     1861 ccgcaatcct gacgcgcacg agccatagcc ggggaatcaa tcctggctgc agggcctcga
     1921 cgacgacagc gcgcaggccc aggccgcctc ctcgccagcc cagaaacagg ctgagcaaca
     1981 ggacgcagcg caggcgcagg gccagcaggc cggctctcag cctgcgccgg cacggc
//
LOCUS       DAOSPL010000939         2035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_55218_length_2035_cov_3.959596, whole genome shotgun sequence.
ACCESSION   DAOSPL010000939 DAOSPL010000000
VERSION     DAOSPL010000939.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55218_length_2035_cov_3.959596"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PLQ54_15415"
     CDS             complement(<1..558)
                     /locus_tag="PLQ54_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94708.1"
                     /translation="MGQLSLHIPGIVCALLSLGSTSSAAAGTPAVAGVRALPDALEVH
                     LAGRLPSARLVELRPYEQYTAEASGAVAWAGRVAGQSLRLPRFADGRDRLYSKFQLIN
                     GATLRAVGVPRWVTDLEALPAERFPMPWPKSKKGVTCPVDMDDLHALGVTTVALTPSA
                     DVDLRDLDPRDLGPGPVAGVVGAEGP"
     gene            complement(546..1508)
                     /locus_tag="PLQ54_15420"
     CDS             complement(546..1508)
                     /locus_tag="PLQ54_15420"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOS94709.1"
                     /translation="MKILQVTSPTTFEILDVPIPTPGPGELLMRVDAVNTCVQWDLHL
                     RHNEPMFVGHQFVYPYTPGQPGHEATGYVETVGPGVTQFAPGDRICAWRDQGHDLWGC
                     YAQYVILKVESAIKIPEGLPAENWASIELAMCLSTAMLDLKRVDAIAGKRFGVTGLGP
                     AGLIAVQIAKAEGASEVIGFDFIPERREMALSLGADACYDPRTVTEAEMPRRMGVYAG
                     IDCHGAKDSVEWLMDHTAAVVALFGVQREDYVYSLGRVGLTILGYRGHFYESAEYARD
                     LIAAGKLDMAAFSNVSLPLERYGEGIDLLDQRKALKICYRPWDS"
     gene            complement(1538..>2035)
                     /locus_tag="PLQ54_15425"
     CDS             complement(1538..>2035)
                     /locus_tag="PLQ54_15425"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS94710.1"
                     /translation="GISELSEGDGTHQVLQKAVPTGAFDVVMLTLNLMLQTAETTVIP
                     LAHQHDVGTVVMMALNQSSGHSGLTSRHAARELVHRYIAAGQLPNDPPYTDPELLDFL
                     EPYSIPEAAIRYVLAHDVTTCCVGMRTPERLAANLRAIDPPYMDLERLRKLRELFGRI
                     QWQTF"
BASE COUNT          317 a          646 c          741 g          331 t
ORIGIN      
        1 cgggccctcg gcgcccacca ccccggccac cggccccggg ccgaggtctc ggggatcgag
       61 gtcacggagg tcgacgtcgg ccgacggggt gagggccacc gtggtcaccc cgagggcgtg
      121 caggtcgtcc atatcgactg ggcacgtgac gcccttcttg ctcttcggcc aaggcatggg
      181 gaagcgctcg gccgggagcg cctcaaggtc cgtcacccac cgtggcaccc cgaccgcccg
      241 cagcgtggcg ccgttgatga gctggaactt gctgtagagg cggtcgcggc cgtcggcgaa
      301 gcgcgggagc ctgaggctct gccccgccac ccggccggcc caggcgactg cgcccgaagc
      361 ctcggcggtg tactgctcgt acgggcgcag ctctaccagg cgcgccgatg gcagtctgcc
      421 ggccagatgg acctcgagcg catccgggag agcgcggacg ccggctacgg ccggcgtgcc
      481 cgccgctgca ctcgatgtgg agccaagcga caggagggca cagacgatcc cggggatgtg
      541 caggctcagc tgtcccacgg ccggtagcag atcttgaggg ccttgcgttg gtcgagcagg
      601 tcgatgcctt cgccgtagcg ttccaggggc agagacacgt tcgagaatgc cgccatgtcg
      661 agcttgccgg cggcgatgag gtcgcgcgcg tactcggcgg actcgtagaa gtgcccgcgg
      721 tagccgagga tcgtcagccc cacgcggccg agggagtaga cgtagtcctc gcgctggacg
      781 ccaaacagcg ccaccacggc ggccgtgtgg tccatgagcc actcgacgga gtctttggcg
      841 ccgtggcagt cgattccggc atagacgccc atgcgcctcg gcatctcggc ctcggtgacg
      901 gttcgcggat cgtagcaggc atcggcgccg aggctgaggg ccatctcgcg gcgctcgggg
      961 atgaagtcga agccgatgac ctccgaggcg ccctcggcct tggcgatctg cacggcgatg
     1021 agccctgccg ggccaaggcc ggtgacaccg aaccgcttgc cggcgatggc atcgacgcgc
     1081 ttgaggtcga gcatggctgt cgaaaggcac atggcgagtt cgatggaagc ccagttctcc
     1141 gcgggcaggc cttcgggtat cttgatggcg ctttcgacct tcaggatcac gtattgggca
     1201 tagcatcccc acaggtcgtg gccctggtcg cgccaggcgc agatgcggtc gccgggggcg
     1261 aactgcgtca cgccgggacc gacggtctcg acgtagccgg tggcttcgtg gcccggctgg
     1321 cccggggtgt aggggtagac gaactggtgc ccgacgaaca tgggctcgtt gtgccgcagg
     1381 tggaggtccc actggacaca ggtgttgacg gcgtccacgc gcatgagcag ctcccccggg
     1441 ccgggggtgg ggatgggcac atcgaggatc tcgaaggtgg tcggtgaggt gacttgcagg
     1501 atcttcattc gtttggtctc tccaagagtc acggcactca gaacgtctgc cactggattc
     1561 gcccgaacag ctcgcgtagc ttccgaaggc gctccaggtc catatagggc ggatcgatgg
     1621 cgcgcaggtt ggcggcgagg cgctcgggtg tcctcatccc cacgcagcac gtggtgacgt
     1681 catgggccag cacgtaccgg atggcggcct cggggatgct atagggttcg aggaagtcca
     1741 gcagctcagg atcggtatag ggcggatcgt ttggcaactg ccctgccgcg atgtaccggt
     1801 gcaccagctc gcgggcggcg tgccggctcg tgaggcccga gtggcccgac gactggttga
     1861 gggccatcat gaccaccgtg cccacatcgt gctggtgagc cagcgggatc acggtggtct
     1921 cggccgtctg cagcatcagg ttgagggtga gcatgaccac gtcgaaggcc ccagtgggca
     1981 cggccttctg gaggacctgg tgggtgccat cgccctccga cagctcgctg atgcc
//
LOCUS       DAOSPL010000940         2035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_55228_length_2035_cov_3.130303, whole genome shotgun sequence.
ACCESSION   DAOSPL010000940 DAOSPL010000000
VERSION     DAOSPL010000940.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55228_length_2035_cov_3.130303"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            436..511
                     /locus_tag="PLQ54_15430"
     tRNA            436..511
                     /locus_tag="PLQ54_15430"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:469..471,aa:Arg,seq:cct)
     gene            477..938
                     /locus_tag="PLQ54_15435"
     CDS             477..938
                     /locus_tag="PLQ54_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008733972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS94711.1"
                     /translation="MCRKFESCWGRQLSGFAQGHATPDAAMLYQHATRDRDQQLAENL
                     SRLAKRTPGPAITMSAVARLDAPAGLQRGRPSQLPCILLARLAVDQACQGAGLGWELL
                     RDVRLRAVRLSDSIGAAAVLVHCRDDKAKEFYLRHRADAAEALDRLADGIR"
     gene            999..1466
                     /locus_tag="PLQ54_15440"
     CDS             999..1466
                     /locus_tag="PLQ54_15440"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOS94712.1"
                     /translation="MGDDTDPTSTAELNDQEEEFLRALARVLVYVPRAFASDLGRRHG
                     LSQSEFFALMHLSESAGGRLRMGDLAARSALTLGAMTRVAKLLEAKGLVERIPVQTDR
                     RACEAALTELGNSRLIELRPVLVASARRRLFDKLQGVDLRVCTAALSRIAEDH"
     gene            1488..>2035
                     /locus_tag="PLQ54_15445"
     CDS             1488..>2035
                     /locus_tag="PLQ54_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94713.1"
                     /translation="MSTTRVSEYDAFGPWVDEVSVPEDVPRLFRDHALDLDTARLVLK
                     VPRDIARRDASPDMDLYDYLLILDSSCLTVLKRHGAGLPGRAEAGPRGYDTMTVPFEQ
                     VVAIRDVVNLLDATVTISTSTGATIPVRYNGSARANVNRLVDELRAVATVDPPGAVGQ
                     ALLAAAPTVRGGTVRLELGAADL"
BASE COUNT          347 a          710 c          654 g          324 t
ORIGIN      
        1 tcgcctgggc ccgatcgagt ggggagtccg ccatggcgca caatctagcg gcgcgctggg
       61 gcttaaacgt aaaggagagc gacgccggtc gaagggcaca aagccgtcta cccaccccca
      121 ggagacatca tctacagggc gtcgctctcg aggtaaacca tacgccatcc gtccggagat
      181 gcaagccgcc cgaggggatg aacttcgcgg ccccctggag tgggagggga ggctcccccc
      241 ggggaagcct cccctccccc ccatcacacc ttgtcaacgc gattccggga gctctggacc
      301 ggtcgataac aaatgcgcag gagttaacgc ttctcggcgc gcccgagccc gcgctcgacc
      361 ccgggcacac cgccgggccg accggacgcc accccgtcgc cggttccact accattcctg
      421 tggcgtcttt cgcctggccc catagctcag gggatagagc agaggtttcc taaaccttgt
      481 gtcgcaagtt cgaatcttgc tggggtcgcc aactatcagg cttcgcgcag gggcacgcca
      541 cgcccgacgc ggccatgctc taccagcacg ccacccgcga ccgagatcag caactcgcgg
      601 agaacctcag tcgcctcgct aagcggaccc ctggccccgc gatcaccatg tccgccgtgg
      661 ccaggcttga cgccccggcg gggctgcaga ggggacgccc ctcgcagctc ccctgcatcc
      721 tcctggcacg tctggccgtg gaccaggcgt gccagggtgc ggggctcggc tgggaactcc
      781 tccgcgacgt gcgcctgcgg gcagtccggc tgagcgacag catcggcgcg gcagctgtgc
      841 tcgtccactg ccgcgacgac aaggccaaag agttctacct tcgccaccgg gcagacgcgg
      901 cagaggccct cgacagactg gccgacggca tccgctgaag gatcatcccc ggcccgcacg
      961 cccggccggg cacaagcttc agtactaaag tactttgggt gggagacgac accgacccga
     1021 catcaactgc ggaactgaac gaccaggaag aggagttcct gcgcgcgttg gcccgggtgt
     1081 tggtgtacgt cccccgggcg ttcgcgtccg acctgggccg tcgacatggc ctgtcgcaga
     1141 gcgagttctt cgctttgatg cacctgtccg agtctgctgg cggtcgtcta cgcatgggcg
     1201 atctggccgc ccgaagcgcc ctcacgttgg gggccatgac cagggtggcc aagctcctcg
     1261 aagccaaggg cttggtggag cgtatccccg tccaaaccga ccgccgggca tgcgaggcgg
     1321 ccctgaccga gctcgggaac agccgactga tcgagttgcg gccggtgctg gtggcgagtg
     1381 cccgccgtcg cctcttcgac aagcttcaag gagtcgacct gcgcgtctgc accgcggctc
     1441 tgtcccgcat cgccgaggac cactgagtcc tcagaacagg agttcccttg agcaccacgc
     1501 gagtcagcga gtacgacgct ttcggcccat gggtcgatga ggtgtccgtg cccgaggacg
     1561 tcccacgtct gtttcgagac catgcactgg acctcgatac agcccgcctg gtgctgaagg
     1621 tcccccgcga catcgcccgc cgggacgcga gtcccgacat ggatctctac gactacctcc
     1681 tgatcctcga ctcgagttgc ctcaccgtcc tgaaacggca cggggcaggc ttgccgggtc
     1741 gggccgaggc cggcccccgc gggtacgaca cgatgacggt gccgttcgag caggtggtcg
     1801 cgatccgcga cgtggtgaat ctcctcgatg cgactgtgac gatctccacc tccacggggg
     1861 cgacgattcc ggttcggtac aacggctcag cgcgagccaa tgtgaacagg ctggtggacg
     1921 agcttcgcgc cgtcgccacg gtcgacccgc caggtgccgt cggccaagcg ctgctcgctg
     1981 cagcaccgac ggttcgaggt ggaaccgtcc gactcgagct aggggcggcc gacct
//
LOCUS       DAOSPL010000941         2034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_55268_length_2034_cov_3.360283, whole genome shotgun sequence.
ACCESSION   DAOSPL010000941 DAOSPL010000000
VERSION     DAOSPL010000941.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55268_length_2034_cov_3.360283"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLQ54_15450"
     CDS             <1..198
                     /locus_tag="PLQ54_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94714.1"
                     /translation="HTEWHRRGRFCELGAGNIGLDNGAVVRALAEAGYAGTVCVEQDT
                     HLRDPLEDLAVSRRHLAGLGL"
     gene            217..1908
                     /locus_tag="PLQ54_15455"
     CDS             217..1908
                     /locus_tag="PLQ54_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94715.1"
                     /translation="MKRVLLWGAGTIIALATAAAAQDAKLPYRWMYVSRNLSSDEQLA
                     GIEDLVRAGAEHGINGMVLSADLELLDRQPEANVERLRRLRAFCDEQGVEIIPIICSA
                     GYGGSVLARNPNLAEGIPVEDALFVAHGDTAVLEPDPPITFANGGFEEYEGDEFAGFA
                     FIDKPGVISFADAGVKHSGDASLRFEAIAAGDPTHGHARAMQELSVAPRRHYVVRAWV
                     KTEDFAAADRFAIQVYAGERAIAPVSVSVPPTTDWMPIAISFNSLAYDRVRIYLGVWG
                     GTTGQMWLDDIAVEEAGLSNVLRRPGTPIVVRGEASGIEYEEGRDFARIEDPRLGHYS
                     DEHDGPSIQLLPGGRITDGERLRVSFYHGARVNEGQVTVCMSEPEVYEIWRDAIRRIE
                     EVLHPPIYFLSMDEVRAGGTCAACKARGLSMAEILGDCITRQFQMIREVKPGAEVVVW
                     SDMLDPNHNAHGDYYLVEGDFSGSWKHVPSDLIMACWDYDVRDKSLSFFSGLGFRTLG
                     GAYYDGNDLTNPAGWIDSLLATPNAAGIMYTTWQDKYALLGGFGDLVREKAGPAE"
BASE COUNT          333 a          648 c          717 g          336 t
ORIGIN      
        1 cacaccgagt ggcaccggcg gggccggttc tgcgagctgg gagcgggcaa catcgggctg
       61 gacaacggcg cggtggtccg cgccctggcc gaggcaggct acgccgggac ggtgtgcgtc
      121 gagcaggaca ctcacctgcg cgacccgctc gaggatctgg ccgtgagccg ccgccacctc
      181 gccgggttgg gcctgtgatc gagaggagca tcctgcatga agcgagttct gctgtggggc
      241 gcgggcacca tcattgcgct cgccactgcg gctgccgcgc aggacgccaa gttgccctac
      301 cgctggatgt acgtatcgag gaacctgtcg tcggacgagc agctcgccgg catcgaagat
      361 ctcgtccgtg ccggcgcgga acacggcatc aacggcatgg tgctatcggc tgacctcgag
      421 ctcctcgacc ggcagcccga ggccaacgtc gaacgcctca ggcgtctgag agctttctgc
      481 gacgagcaag gggttgagat catccccatc atctgctcgg caggctacgg gggctcggtg
      541 ctcgcgcgga acccgaacct ggccgagggc attccggtcg aggacgcgct gttcgtcgcg
      601 cacggtgaca cggccgtgct cgagcctgac ccgccgatca cgttcgccaa cggggggttc
      661 gaggagtacg agggcgacga gttcgccggc ttcgccttca tcgacaagcc cggcgtgatc
      721 tcgttcgccg acgccggcgt gaagcactca ggcgacgcct cgctgcgatt cgaggccatc
      781 gccgcgggcg atccgacaca cggccacgcc cgggcgatgc aggagctgag cgtggcgccc
      841 cgcaggcact acgttgttcg cgcctgggtg aagaccgagg acttcgccgc ggccgatcgg
      901 ttcgccatcc aggtctatgc cggagagcgc gctatcgcgc cggtcagcgt gtcggtaccg
      961 ccgacgacgg actggatgcc catcgccatc tcgttcaaca gcctcgccta cgacagggtg
     1021 cggatctatc tcggtgtctg ggggggcacg acgggccaga tgtggctcga cgacatcgcg
     1081 gtcgaggagg cggggctgtc caacgtgctc cggcgcccgg ggacgccgat cgtggtccgc
     1141 ggcgaggcga gtggcatcga gtatgaggag ggacgcgact tcgcccggat cgaggacccg
     1201 cggcttggcc actattccga tgaacatgat ggcccctcca tccagctcct acccgggggc
     1261 cgtatcacag acggcgaacg cctccgcgtg agcttctacc acggggcgcg tgtaaatgag
     1321 ggccaggtca cggtatgcat gtcggagccg gaggtctacg agatctggcg cgacgcgatc
     1381 cgccgtatcg aagaggtgct gcatcccccg atctacttcc tgagcatgga cgaagtgcgt
     1441 gccgggggaa cctgcgccgc ctgcaaggcg cggggcttga gcatggcaga gatcctcggc
     1501 gactgcatta cgcggcagtt ccagatgatc cgcgaggtca agcccggggc cgaggtggtc
     1561 gtctggtcgg acatgctcga cccgaaccac aacgcccacg gcgactacta cctggttgag
     1621 ggcgacttct cgggatcgtg gaagcatgtc ccgagcgatc tgatcatggc gtgctgggac
     1681 tacgacgtgc gcgacaagag cctgtcgttc ttctccgggt tgggtttccg gaccctcggc
     1741 ggggcgtact acgacggcaa tgacctgacg aacccggcgg ggtggatcga ctcgctcttg
     1801 gcgacgccga atgcggcggg catcatgtac accacctggc aggacaagta cgcgctcctg
     1861 ggcggtttcg gcgacctcgt acgcgagaag gcgggaccgg cagagtaggg cagctgctgc
     1921 gtattcgccg ccccgcccgt ctgacagaca tgctggaggc tgcggcatgc atggctggct
     1981 gatcgtggcg ctcgtgctgt tcggggtgat agcggtggca ttcagcctgc cccc
//
LOCUS       DAOSPL010000942         2027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_55508_length_2027_cov_3.443205, whole genome shotgun sequence.
ACCESSION   DAOSPL010000942 DAOSPL010000000
VERSION     DAOSPL010000942.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55508_length_2027_cov_3.443205"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..1868
                     /locus_tag="PLQ54_15460"
     CDS             333..1868
                     /locus_tag="PLQ54_15460"
                     /inference="COORDINATES: protein motif:HMM:NF037707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HOS94716.1"
                     /translation="METSAACGVLWGKSAADGRPNLLVQHLLDTAAVAELIWDRFLAA
                     SLKERLNRCCQGRGGVMYEWLCGVHDVGKASPAFQIKVPELAQRLPDAGLTWERLTAS
                     ERGWHHALAGAVIVRRVLADAWSSAAVSWVWPIIAGHHGSVPGPGKLRPPGYARALGS
                     KPWLHAQDDLVATIADALGVSLPSLCPTATPRRAEQLALLGAVVMADWIASDERNFPG
                     ICDLPQVSMDVARRRAERAWNKLMLRGGWAPSQLSTGPTVFTDRFHVAPRPVQRAAVD
                     VAASIPSPGIIVVEAPTGEGKTEAALAAAEVLARHFGSDGVFVGMPTQATADAMYSRV
                     RTWAQGIDAGVPLALLHGKRRFNKEWRAIEDSVRFVATEDDWYGVESETSLPVTAPAE
                     WFLGRKRGLLTPLAVGTIDQLLHAATRTKHVMLQHTGLAGRVVMLDEVHAYDVYMSQF
                     LHEGLRWLGDGGVPVVVLSASDYARLTAEAGQNRGRFADHVLAFPGGDFDRVAAKPRD
                     VSF"
     gene            1865..>2027
                     /locus_tag="PLQ54_15465"
     CDS             1865..>2027
                     /locus_tag="PLQ54_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008887440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOS94717.1"
                     /translation="MIYLLDTNVLSALRRPDRAPQVARWLADKPDDSLFLSVITLGEV
                     ERGIALQSPR"
BASE COUNT          317 a          642 c          687 g          381 t
ORIGIN      
        1 ggcgcatcaa ggagacgccg tacctgtggc tgcgcgaggt gccggcgttc tgggtgtatt
       61 acctgcgcgg gctgatgggg tgagggcgcc cgcgctctgg cgctgcccat tgggctcccg
      121 gcacttacga gctcgaacag gggctgccgg ccctatgggg gaaggctggg ctcacgtcgc
      181 ccaggggctg taggtcaagt cgatgcacgt acccggtgtc ttgttcgtgt agctcttccg
      241 tccgcacctg cggctatgat tgaaacagtg atcaccaact ggtaggacca ctcgccacag
      301 agggggtgtc cgccgttcag gcagcggggg ccatggagac aagtgccgcc tgcggagtgc
      361 tctgggggaa gtccgcggcc gatggtaggc cgaacctgct cgtgcagcac ttgctcgata
      421 cggcggctgt ggccgagctc atctgggacc gattccttgc tgcatccctc aaggaacggc
      481 tgaatcgctg ttgccagggc cgcggtggcg tcatgtacga atggctgtgc ggcgtacatg
      541 acgtcggcaa ggcatctccg gcgtttcaga tcaaggtgcc agagcttgcc cagaggctgc
      601 ccgacgccgg actcacttgg gaacgactca ccgcgtccga acgaggctgg catcatgcgt
      661 tggcgggcgc agtaatagtc cgccgcgtgc ttgccgatgc ttggagcagc gccgcggttt
      721 cttgggtctg gcccatcatt gcgggtcacc atgggtcggt gcccggcccc ggcaagcttc
      781 gtcctcccgg ttatgctcgc gccctgggat ccaagccgtg gctgcacgcg caggatgatc
      841 tggttgcgac gattgccgat gccttgggcg tttcgctacc gtctctgtgc ccgactgcaa
      901 cgccccgcag ggccgagcag ttggcgctgc tgggcgcggt cgtgatggca gactggatcg
      961 cgagtgacga acgtaacttc ccgggtatct gcgatctgcc gcaggtgtcc atggatgtgg
     1021 cgagacggcg cgctgaacga gcttggaaca agctgatgct tcgcggcgga tgggcgccaa
     1081 gccaactgag caccggcccc acggtcttca cagaccgctt ccatgtcgcg ccgagaccgg
     1141 tccaacgcgc ggctgtcgac gtggcggcat caatcccgtc gccggggatc atcgtcgtcg
     1201 aggctccgac cggagagggc aagaccgagg cagcccttgc tgccgccgag gtactcgctc
     1261 gtcatttcgg tagcgacggc gtgttcgtag ggatgcctac gcaggcgacg gccgacgcca
     1321 tgtactcccg cgtccggaca tgggcacagg gaatagatgc gggcgtgccg ctcgccctcc
     1381 tccatggcaa gcggaggttc aacaaggagt ggcgcgcgat tgaggactcc gtgcgcttcg
     1441 tggccacaga ggacgattgg tatggggttg aatcagagac cagtctcccg gtgacggcgc
     1501 ctgcagagtg gttccttggc cgcaagcgtg gtctacttac gcccctcgcg gtgggcacca
     1561 tagaccaact tctccacgca gcgacgcgca cgaagcacgt catgctccag cacacaggtc
     1621 tcgccggtcg cgttgtcatg ctcgatgaag tacatgccta cgacgtctac atgtcccagt
     1681 ttctccacga gggccttcgt tggctaggtg acggcggcgt accggtcgtc gtcctttcgg
     1741 ccagcgacta cgcgcggctg acggcggagg ccgggcagaa ccggggccgc tttgccgatc
     1801 atgtgctggc ctttccggga ggcgacttcg accgggtcgc cgccaagccc cgcgacgtca
     1861 gcttttgatc tacctcctcg acaccaacgt gttgtccgcc ctccgccgcc cggaccgcgc
     1921 gccccaggtg gcgcgctggt tggcggacaa gccggatgac agcctgttcc tgtcggtcat
     1981 caccctgggc gaggtcgagc gtggcatcgc cctccagtcc ccgcgcg
//
LOCUS       DAOSPL010000943         2023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_55688_length_2023_cov_2.559451, whole genome shotgun sequence.
ACCESSION   DAOSPL010000943 DAOSPL010000000
VERSION     DAOSPL010000943.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55688_length_2023_cov_2.559451"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PLQ54_15470"
     CDS             <1..386
                     /locus_tag="PLQ54_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94718.1"
                     /translation="PGWHSHAISDLDDIDLCETLFIFFQGCSSAKEVGGIRELCAAVT
                     NKGAQAYAGFEGHILSEFFSTFASEFWECASRQGNTISEARVEAVWAVADANSGEDAG
                     YASFWCTNPWLELFPPRFSDACVGP"
     gene            432..1271
                     /locus_tag="PLQ54_15475"
     CDS             432..1271
                     /locus_tag="PLQ54_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94719.1"
                     /translation="MRRMSLRLAPSRWRRLRVLLVVGLALVAVATAHIGRGWTTESNA
                     DRGVALATKLLPTIYARPMNDLHAEVQPRTARREGDCRVAARWTDDDGSKRRASLTVD
                     VNREEIRTYVGPPLTDLPDPSSDGRLKTAEQFARAHLGPFTDATQLVSEKQSMDVLFM
                     YTWANVLPSGAWTGDWWSVEVDTNPKAGGVHAFIQQHAPRCVTEDEVKLSRDDAIEAA
                     RKALRRDGGGTQAVEVLETRLVLSSPNSPTQGPVWHVTFSEAGAARRGFQIDGITGKV
                     LRP"
     gene            1268..1402
                     /locus_tag="PLQ54_15480"
     CDS             1268..1402
                     /locus_tag="PLQ54_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94720.1"
                     /translation="MNASTAGRLRRRSWAIAAAAAALLALVLFGRPPESTRREEARLR
                     "
     gene            1550..>2023
                     /locus_tag="PLQ54_15485"
     CDS             1550..>2023
                     /locus_tag="PLQ54_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94721.1"
                     /translation="MVLEALRSESYGRAWSAALAISGNIRTEMHDEADQVGVCLESEG
                     DPGIRHRIATALASTGNTEAMRRHLSTALIAEPVRRNRLWYARLLWEHDLGPVEARLL
                     TSRADGKVPSSDSAAIAELRKELANGGQEAADALCFSGHLDLLSAEQKAGLAGRPG"
BASE COUNT          334 a          630 c          728 g          331 t
ORIGIN      
        1 atcctggatg gcactcgcac gccattagtg atctcgatga tatcgatcta tgtgagacac
       61 tgttcatctt cttccagggc tgctcctctg ccaaggaggt cggtgggatt cgggagctat
      121 gtgcagcggt caccaacaag ggagcccagg cctacgctgg gttcgagggg cacatcctct
      181 ctgagttctt cagcacgttc gcgtccgagt tctgggagtg cgcgtcacgg cagggcaaca
      241 cgatctcgga ggcccgggta gaggccgtct gggctgtcgc ggacgccaac agcggggagg
      301 atgccgggta tgcgtcattc tggtgcacca acccgtggct ggagttgttc cccccacggt
      361 tctcagacgc ttgcgttggc ccgtagcgac ggaagaagcg cctcgtcgtg cgacctggga
      421 aggggtgcgg tatgcgtcga atgtcattgc gtctcgcgcc gtctcgatgg cggcgcctcc
      481 gggtactact cgttgtgggg ctcgcgctcg tcgccgtcgc cacagcccac atcggccgcg
      541 gctggaccac ggagagtaac gcagaccgtg gtgtcgcgct tgccacgaag ttgctaccca
      601 caatctacgc gcgcccgatg aacgacctgc atgccgaggt acagccgcgc accgcgcgcc
      661 gcgagggtga ctgccgcgta gcggccaggt ggaccgacga cgacgggtcc aagcgcaggg
      721 cgtcgctgac agttgatgtc aatcgcgagg agatccgcac ctacgtgggg cctccgctga
      781 cggacctgcc cgacccatcc tcagacgggc gcctgaagac tgccgagcag tttgcccggg
      841 cgcacctcgg gccgttcacc gacgccaccc agctcgtctc cgagaagcag tcaatggacg
      901 tgctcttcat gtacacatgg gcgaacgtgc tgcccagtgg agcatggacg ggagactggt
      961 ggtccgtgga ggtcgacacg aacccgaaag ccggaggcgt gcatgcgttc atccagcagc
     1021 atgcgccccg atgcgtcacg gaagacgagg tgaagctgtc gcgcgacgac gcgatcgagg
     1081 ctgccaggaa ggcgctgcgc cgagatgggg gaggcacaca ggcggttgag gtgctggaga
     1141 cgcgactggt gctaagctca cccaactcgc cgacgcaagg gcccgtgtgg cacgtgacgt
     1201 tctccgaggc cggggcggct cgtcgcggct tccagatcga cgggatcacc gggaaggtgc
     1261 tgcggccgtg aacgcgagca cggccggccg gcttcggcgg cgctcttggg ccatcgcggc
     1321 cgccgcggcc gccctgctgg ctctcgtgct gttcggccgg ccccccgaaa gtacccgtcg
     1381 cgaggaggcc cgcctgcgct agcgggttcg ggaggtgtcc gcggcgttgg cgtcgccgga
     1441 tgcggaggcg aggatgaggt gcctctgggc tctgtgttcc tcggacctcg ttgccgggga
     1501 ctccatctac gccaatctcg ggcctgtgga cagagcgagg gctaggcagg tggtccttga
     1561 agcgctgcgg tcggaatcgt acggacgggc ttggtcggcc gccttggcga tctccggcaa
     1621 catacggacc gagatgcacg atgaggccga ccaggtgggg gtgtgcctgg aatccgaggg
     1681 ggatcccggg atccgccaca ggatcgccac cgcgctcgcc tcgacaggca acaccgaagc
     1741 catgcggcgg cacctgagca cggccctgat tgctgaaccc gtgcggcgca accggctctg
     1801 gtacgcccgg ctgctgtggg agcacgacct ggggccggta gaagcgcggc tgctcacaag
     1861 ccgggccgat gggaaggtgc catcgagcga ttcggcggcg atcgcggaac tgaggaagga
     1921 gctggcgaac ggaggccagg aagccgccga tgcgctgtgc ttctcggggc atctcgacct
     1981 gctcagcgcg gagcagaagg cgggcctggc cgggcgacct ggt
//
LOCUS       DAOSPL010000944         2023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_55690_length_2023_cov_2.445630, whole genome shotgun sequence.
ACCESSION   DAOSPL010000944 DAOSPL010000000
VERSION     DAOSPL010000944.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55690_length_2023_cov_2.445630"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PLQ54_15490"
     CDS             complement(<1..367)
                     /locus_tag="PLQ54_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94722.1"
                     /translation="MVRLCGVAAWALIVGVSPAGGTHRGAEFPLSREELMTLRDELHI
                     NTLRFFVHPGFIGLPQTTWNGPESIDYTRFAEADYVWHDPTPAVDSLDEVLDLLRELS
                     IHPVLLVFSVDEYVAYIGRD"
     gene            complement(1054..>2023)
                     /locus_tag="PLQ54_15495"
     CDS             complement(1054..>2023)
                     /locus_tag="PLQ54_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94723.1"
                     /translation="GTVPWPKGFDEAYGLAEPLDFARFDDWVSRWPGATHYALFLAVG
                     QSLAGIPMTSEGFPACVASWMHVWMAHVRDLGIHPSQLMLLLVDEPSMEEQEQRIILW
                     AKAIKAAEPEVLIWEDPVHSRPELAALPEMFAVSDVLCPNLGIFASGPESSRSYYERL
                     RTEGKRLWFYQCSGPVRSFDPYYYYRLQHWYCFKYGATGSGFWAYGDTGGTANSWNDL
                     TASGASFTPVYLDAKSVTNGKHFEAVREGLEDYEYLRMLRDRVREEAAKGNVRASETF
                     RTLLARAIDEVCGAGYEPGLVAWTVDKDRSTADRVRIQILEALTAP"
BASE COUNT          320 a          716 c          675 g          312 t
ORIGIN      
        1 cgtcccgccc gatatacgcc acgtactcat ccaccgagaa caccagcagc acggggtgga
       61 tggacagctc gcgcaggaga tcgagcacct catcgagcga atcgacagcc ggcgttggat
      121 cgtgccacac gtagtcggct tctgcgaaac gcgtgtagtc gatcgactcg ggcccgttcc
      181 acgttgtctg cggtagcccg atgaagccgg gatgcacgaa gaacctgagc gtgttgatgt
      241 gcagctcgtc gcgcagtgtc atgagctcct cgcgcgacag cgggaactcc gccccccgat
      301 gcgtcccgcc ggccgggctg acacccacga tcaaggccca cgccgcaact ccgcacaggc
      361 ggaccatggc gaagccccct ccccgcgacg ccgctctgct tcggcgcgcc acagagggtc
      421 gcctgcgctc ttgggcccgc gccatgcggc agcttggggc tccagcggcc cgtgggcctg
      481 gttgcggagg ggcgccatgg gggcaaaccg ctgcgtggtg gccccccgac acgccttggt
      541 cagccgcggg ccgaccgcgt aggccgatcg tcccggtcgg ccgtgaggtg tcgagcggga
      601 ccctcgacct acgcgcgctc cgggcgcacc ctcgttcggc ccctccacac aacctcgccg
      661 agcccgagga ggggccgcat ggacagggac cgcgaagccg aaagtgaact gcagttcggt
      721 gagtagatgg gggtttgggc ggattggggg caacgcgtgt ttgggttggg ccccgcgaag
      781 cgggggtgta cccgccgctc accgacagac gcggcgaccc cattgccggg gccggagtcg
      841 gcttcccgga cactggtcag tccgatcccg agccaccggg ggaggcggcg agcggtccgt
      901 gcggtcttcg gagcgtcgca taggggagac cgtcggtcga acgacctctg gtgtggcccg
      961 agccggcccg aggccgaacg cggccggcca gcactcaggc gccgcagccc acgttgccac
     1021 cggtcgccgc cgcctctttc ccccacttgc cccctacggt gccgtcagcg cctcgagtat
     1081 ctggatccgg acccggtcgg ccgtcgagcg gtccttgtcc acggtccagg cgaccagccc
     1141 cggctcgtag ccggccccgc acacttcgtc gatggcgcgc gcgagcagcg tgcggaaggt
     1201 ctccgaagcg cgcacgttgc ccttcgcggc ttcctcccgc acccggtcgc gcagcatccg
     1261 gaggtactcg tagtcctcga gtccctcgcg gaccgcctcg aagtgcttgc cgttggtcac
     1321 gctcttggcg tcgaggtaca ccggcgtgaa gctcgcgccc gacgccgtca ggtcgttcca
     1381 cgagttcgct gtaccgcccg tgtcgccgta ggcccagaag ccgctgcccg tcgcgccgta
     1441 cttgaagcag taccagtgct gcagccggta atagtagtag gggtcgaagc tcctcaccgg
     1501 gccgctgcac tggtagaacc acagccgctt cccctcggtc cttaggcgct cgtagtagga
     1561 ccgagagctc tcggggccgc tggcgaagat gcccaagttc gggcacagca cgtcggagac
     1621 cgcgaacatc tccggaaggg ccgccagttc cgggcgcgaa tggacggggt cctcccagat
     1681 gagcacctcg ggctccgccg ccttgatcgc cttggcccag aggatgatcc gctgttcctg
     1741 ctcttccatt gatggctcgt cgacgagaag cagcatgagt tgcgatgggt ggatcccaag
     1801 gtcacggacg tgggccatcc aaacatgcat ccagctggcc acacacgccg ggaacccttc
     1861 cgaggtcatc gggatgccgg ccaacgattg tcccacggcc aggaacaagg cgtagtgggt
     1921 cgcccccggc cagcgcgata cccagtcgtc gaagcgggcg aagtccagcg gctccgcaag
     1981 gccgtacgcc tcgtcgaagc ccttcggcca cggcacagtc ccg
//
LOCUS       DAOSPL010000945         2021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_55778_length_2021_cov_3.374364, whole genome shotgun sequence.
ACCESSION   DAOSPL010000945 DAOSPL010000000
VERSION     DAOSPL010000945.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55778_length_2021_cov_3.374364"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="PLQ54_15500"
     CDS             <1..1219
                     /locus_tag="PLQ54_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOS94724.1"
                     /translation="PTPPAPRAKSVIQIWMWGGPSHLDTFDPKPEAGRDYCGPLDHPI
                     ETNVPGVFVGELLPQLARLADQYSIIRSMTHRVNAHETASYIVQTGREPGGSVYPSAG
                     AVVSLFRGYDHGYEGLIPPYIVLTQSQGRFSEAGFLGARYKPFATGGDPNQKVFAVEG
                     IVSEGVTAERQQARRDLLHSLDSLGAAMPGEKLFEQLDGCEAKAYDLILGQGGKVFDL
                     GEESPEMRDRYGRNTFGQSCLVARRLVEHGVPYVTINYQGWDTHKRHFETMRQKLPQM
                     DQGMAALIGDLSERGLLDSTIVWWGGEFGRTPRVQWEEPWNGGRGHYGNCFSTVVAGG
                     GFRGGLVVGESDATGSEVLERPVHPSEVIRSIYTLLGIDPDGPMPMRGAEGGSLLPAP
                     AGVAGGGLLAELM"
     gene            1227..>2021
                     /locus_tag="PLQ54_15505"
     CDS             1227..>2021
                     /locus_tag="PLQ54_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94725.1"
                     /translation="MSALLLWASACWGQADIRSPHVGYLYPAGGQRGGTVRVIVGGQF
                     LRGVNAAHVSGAGVRASVARPSGPPTPLNKDLLEEVARHFSEAAEKRVAEIPEDQRGP
                     AHTLGVLRRRVPPRAPDAKPLNLPDHPLLRDLDSKTLPELVELARWFFSPRNLQPPQR
                     AIAETVLLEVRIDANAVPGGREIRLAGPMGLSNPLRFEVGAAPELLESEPNDRPGSRQ
                     QTAESAFVLNGQVLAGDVDRVRFAARQGERLKIEVHARRLMPYLADA"
BASE COUNT          309 a          619 c          774 g          319 t
ORIGIN      
        1 gccgacgccg ccggctccca gggccaagtc cgtgatccag atctggatgt ggggggggcc
       61 atcgcacctg gacaccttcg acccgaagcc ggaagccggg cgggactact gcgggccgct
      121 agaccatccg atagagacga acgtgcccgg ggtcttcgtc ggtgagctct tgcctcagtt
      181 ggccaggctg gcggaccagt attcgatcat ccgcagcatg actcacaggg tcaacgcaca
      241 cgagacggcg tcgtacatcg tccagacggg acgcgagccc ggcgggagcg tgtatccgtc
      301 ggccggtgcg gtcgtgtcgt tgttcagggg ctacgaccac gggtatgagg gcttgattcc
      361 gccctacatc gtgctcaccc agtcgcaggg acggttttcg gaggccgggt tcctcggcgc
      421 ccggtacaag ccgttcgcga ccggtggcga ccccaaccag aaggtgttcg ccgtcgaggg
      481 gatcgtgtcc gagggcgtga cggccgagcg acagcaggcg cggcgcgact tgctgcactc
      541 gctggactca ctgggggccg cgatgccggg cgagaagctg ttcgagcagc tcgacggctg
      601 tgaggcgaag gcgtacgacc tgatcctcgg gcaggggggg aaggtgttcg acctgggcga
      661 ggagtcgccc gagatgcggg atcggtatgg acggaacaca ttcgggcagt cgtgtctggt
      721 ggcgcggcgg ctggtggagc acggggtgcc ttacgtgacc atcaactacc aggggtggga
      781 cacacacaag cggcacttcg agaccatgcg tcagaagctg ccccagatgg atcagggcat
      841 ggccgcgctg atcggcgact tgtcggagcg ggggctactc gacagcacca tcgtatggtg
      901 gggcggggag tttgggcgga ccccgcgcgt gcagtgggag gagccgtgga acggcgggcg
      961 gggccattac ggcaactgct tctcgacggt ggtcgccggc ggcgggttcc ggggcgggct
     1021 ggtggtcggg gagtcggatg ccacgggatc cgaggttctg gagcggccgg tgcatccgag
     1081 cgaggtcatc cggagcatct acacgttgct ggggatcgac ccggacggcc cgatgccgat
     1141 gcgcggtgcc gaaggcgggt cgctgcttcc cgcgccggcc ggggtcgcgg ggggtgggct
     1201 gcttgcggag ctcatgtagg gccacgttgt ccgcgcttct cctgtgggcc agtgcgtgct
     1261 ggggccaggc ggacatccga tcgccccacg tggggtactt gtacccagcg ggcgggcagc
     1321 gtggcggcac ggtgcgggtg atcgtcgggg gccagttctt gcgcggggtc aacgcggctc
     1381 atgtgtcggg tgcgggggta cgggcttcgg tggcgcggcc gagcggcccg ccaacccctc
     1441 tcaacaagga cctgctcgag gaagtggctc gccacttttc cgaggcggcg gagaagcgcg
     1501 ttgccgagat accggaagac cagcggggtc cggcacacac gctcggcgtc ctgcggcgcc
     1561 gggtcccgcc gcgcgcgccc gatgcaaagc cgctgaacct gcccgatcac ccgttgcttc
     1621 gcgatctgga cagcaagacg ttgccggagt tggtggagct ggcccggtgg ttcttcagcc
     1681 cacgaaacct ccaaccgccc cagcgggcta tcgccgagac ggtgctgctc gaggtccgca
     1741 tcgacgcgaa cgcggtgccc ggcggccgcg agatccgact ggcgggtccc atggggctgt
     1801 cgaacccgtt gcgctttgag gttggtgcgg cgccggaact gctcgagagc gagcccaacg
     1861 accgaccggg gagtcggcaa cagacggcgg agtccgcctt cgtcctgaac ggacaggtcc
     1921 tggccggcga tgtcgaccga gtccgcttcg cggcgcggca aggggagcgg ctgaagatcg
     1981 aagtccacgc gcgccggctc atgccctatc tggccgacgc g
//
LOCUS       DAOSPL010000946         2018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_55895_length_2018_cov_3.010698, whole genome shotgun sequence.
ACCESSION   DAOSPL010000946 DAOSPL010000000
VERSION     DAOSPL010000946.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55895_length_2018_cov_3.010698"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="PLQ54_15510"
     CDS             <1..1341
                     /locus_tag="PLQ54_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94726.1"
                     /translation="VPTQRGLLEERPFDVGQGGSAAFWLTVTATEHARAGQYRLAVRV
                     TSRPRGELLLPLTVEVLPFTLERPADRLSLLYADVGRWRTMSDEQVLAELRDFARHGF
                     TGLVEIPLGEPDLTGIAEGRATFDASPFRKLVALCAQAGLPGPHVCSAGGMPERVAQA
                     LGIEANLHAGEWPEKLKAGLRVVARAAVEATRDAGARWYYYGVDEPSGDNTYAIQDYQ
                     CWRGGGAETYATFYNIGFLEKASQYLTAPCFVVGLVSARDTAREAREACARTGAEFWW
                     YGSGSYVNPFPQEGFMFHNRYATGLLWYKTGARAQVTWTFCRPHEDVFNDFDGSAVNS
                     GEPKDQATAYPHLLKPDDWSTYQGAIPTIAWESIREGVDDYAYLHTLERAIERAKHSA
                     SAPRQRAALRAEEALRSMVDGIPWANPMNSATFSTQRLQDVRHKAARLIAELRG"
     gene            complement(1364..>2018)
                     /locus_tag="PLQ54_15515"
     CDS             complement(1364..>2018)
                     /locus_tag="PLQ54_15515"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOS94727.1"
                     /translation="IVGRAMLCRNGLLEVPHSAELDPPQLTVGAWVRVPAIPSGWDPR
                     QWLVCKAPNEFADGNYSLIIHGDKVGAYLNIGGGQENYHEASSPTGTIRVDTWQHLAM
                     TYDGEALRVFCDGRQVAETAIGKARTTTDVPLTLGGRQDRYGPGFLRGALDDVLLFDR
                     ALTADEIASHHAAGLRGERIQGAAAEWTFDDLPPEPPVARWVRAHAGLEPAYRHLGR"
BASE COUNT          332 a          689 c          696 g          301 t
ORIGIN      
        1 gtgccgaccc agcgaggact ccttgaggaa cggcccttcg atgtgggtca gggcgggagc
       61 gcggctttct ggctgacggt cacggctacc gaacacgccc gggcgggcca gtaccggctg
      121 gcggtacgcg tcacctcccg cccaagaggc gagctactgc tccccctcac ggtcgaggtg
      181 ctgccgttca cgctggagcg ccccgccgac cggctgtcgc tgctctacgc cgacgtgggc
      241 cgatggcgca cgatgagcga cgaacaggtg ctggccgagc tgcgcgactt cgcgcgccac
      301 ggattcacgg gtctggtgga gatccccctc ggcgagccgg atctcaccgg catcgccgag
      361 ggccgggcga cgtttgacgc ctcgccgttc aggaagctcg tggccctctg cgcgcaggcg
      421 gggctgcccg ggccgcatgt gtgtagcgcc ggtgggatgc cggagcgcgt cgcccaggcg
      481 ctcgggatcg aggcgaatct gcacgccggc gagtggcccg agaagctgaa ggcggggctc
      541 cgggtggtgg cgcgggcagc cgtcgaggca accagagacg ccggtgcccg atggtactac
      601 tacggcgtcg atgagccctc aggcgacaac acctacgcca tccaggacta ccagtgctgg
      661 cgcggaggcg gagccgagac gtacgccacg ttctacaaca tcgggttcct cgagaaggcc
      721 agccaatacc tgaccgcgcc gtgctttgtt gtgggtcttg tcagtgcgag ggacacggcc
      781 agggaagccc gagaggcctg cgcgcgcacc ggtgccgagt tctggtggta tggctcgggc
      841 tcctatgtga acccattccc gcaggagggg ttcatgttcc acaatcgcta tgccacgggc
      901 ctgttgtggt acaagaccgg ggcccgggcc caagtgacct ggaccttctg ccggccccac
      961 gaggacgtgt tcaacgactt cgacggctcg gccgtaaact ccggcgaacc caaagaccag
     1021 gccacggcct atccgcacct gctgaaaccc gatgactgga gcacctacca gggcgccatc
     1081 cccaccattg cgtgggagtc catccgcgaa ggcgtcgacg actacgcgta cctccataca
     1141 ctggaacggg ccatcgagcg ggcaaagcac tccgcatccg ccccgcgaca gcgggcggcg
     1201 ttgcgcgccg aggaggcgct gcgctccatg gtcgacggga tcccatgggc caatcccatg
     1261 aacagcgcga cgtttagcac ccagcgcctg caggatgtgc ggcacaaggc agccaggcta
     1321 atcgcagagc tgaggggata gccggccagg agccccatcg ccatcaccgt cccaggtgcc
     1381 ggtacgccgg ctcgagcccc gcgtgggccc gaacccacct cgccactggc ggctcgggcg
     1441 gcaggtcgtc gaatgtccac tcagccgccg cgccctggat ccgctcgccg cgcaacccag
     1501 cggcgtggtg actggcgatc tcgtcggccg tgagggcgcg gtcgaacagc agcacatcat
     1561 cgagcgcgcc gcggaggaac ccggggccgt agcggtcctg ccggccgccg agcgtgaggg
     1621 gcacgtcggt ggtcgtccga gcctttccga tggccgtttc ggcgacttgc cgaccatcgc
     1681 agaacacacg cagcgcttca ccgtcgtacg tcatcgccag gtgctgccag gtgtcgacgc
     1741 ggatggtgcc ggtgggactc gaggcctcgt ggtagttctc ctgcccgccg ccgatattca
     1801 gataggcgcc gaccttgtcg ccatggataa tcaatgagta gttgccatcg gcgaactcgt
     1861 tgggcgcctt gcagaccagc cactggcgcg ggtcccagcc cgatgggatg gccggcacgc
     1921 gcacccaggc cccaaccgtg agctgcgggg ggtcgagctc cgcggagtgc gggacctcca
     1981 gcagcccgtt gcggcacagc atggcgcgcc cgacgatg
//
LOCUS       DAOSPL010000947         2017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_55927_length_2017_cov_3.345056, whole genome shotgun sequence.
ACCESSION   DAOSPL010000947 DAOSPL010000000
VERSION     DAOSPL010000947.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55927_length_2017_cov_3.345056"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLQ54_15520"
     CDS             <1..270
                     /locus_tag="PLQ54_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94728.1"
                     /translation="IEEWEWEGRRHAVPAVTHAAWRAPDGRFGVVLANWTTAARAVHI
                     ADQRLSGAVTEVVCASDTLERERRTDDGALDIALPPLSIALVAGA"
     gene            complement(267..620)
                     /locus_tag="PLQ54_15525"
     CDS             complement(267..620)
                     /locus_tag="PLQ54_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HOS94729.1"
                     /translation="MLKQHVHALALACRHPRTPWYAKAWVALVVAYALSPIDLIPDFV
                     PVLGAVDDLVLMPLGIWVGLRLVPPEVMAECREAAAGTAYRVRGALVAAAVVIVIWLA
                     CAIGIGLWLWAYRRS"
     gene            complement(674..>2017)
                     /locus_tag="PLQ54_15530"
     CDS             complement(674..>2017)
                     /locus_tag="PLQ54_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94730.1"
                     /translation="SLAWSQLWRAIVTRTVPGATVPAPDPPSTPAIHIEPSQRREAYR
                     RALDRNLLWFVRSGIMPRADGSLGVHSALALSALFGGPVEELASSYRCDCNAMTLKAL
                     WLYGDVTGDGRWHDRALRMAELLVGHQFSDPSLPRFGGFPWLDVHADASFPWDDNTRI
                     ANVLLWLWERTGDRRWLMPALRNTELARALAREDDGVIAGQWVDPVELDRIGRQSWRD
                     RASGAGYPAFDIQRWAGAALATRDPVYRALARRVCEVHGGSVGSLGMAHAARILGTPD
                     AARSLAGYWARYLADPDVRRYGVMRLRGPGSYEWAFENDCSINTLAGEPLTDQLYQTS
                     WDALAAWAAYKAIGDPACREAFEGIADYLVAIQCADPDPRLDGCWMRGFDFEAWEYFG
                     APYDPNYGPYHAYSGWMNAIIDTALAQYLLDESPYPVGRDPDTVHRVLAEVRAER"
BASE COUNT          354 a          662 c          728 g          273 t
ORIGIN      
        1 atcgaggaat gggagtggga agggcggcgc cacgccgtgc cagcggtcac ccacgcggcc
       61 tggagggcgc ccgatgggcg attcggagta gtcctggcca actggaccac ggccgcgcgg
      121 gcggtgcaca tcgccgacca acgcctgtcc ggcgccgtga ccgaggtggt ctgtgcgtcc
      181 gacacactcg aacgcgagag gcgcacggac gacggagcac tcgacatcgc cctgccgccg
      241 ctgagtatcg cgctggtcgc cggggcctag ctccgccggt atgcccagag ccacaacccg
      301 atgcctatgg cgcaggcgag ccagatgacg atgaccacgg cagcggccac cagtgccccg
      361 cgtacccggt atgccgtacc ggcggcggcc tcgcggcact cggccatcac ctccggcggg
      421 acgaggcgaa ggccaaccca gatacccaag ggcatcagaa cgaggtcgtc caccgcgccg
      481 agcacgggga cgaagtcggg gatcaggtca atcgggctca gcgcgtaggc gacgacgagc
      541 gcgacccacg ccttcgcgta ccacggggtc cgcggatgcc ggcacgcgag cgcgagggcg
      601 tgcacgtgct gcttcagcat gcgaagtcgc acgaggaacg cgggttgcac gcgcgtctcg
      661 ccccctggcg ccgctagcgc tcggcccgga cttccgccag tacacggtgt acggtatctg
      721 ggtcgcggcc gaccgggtat gggctctcgt ccagcaggta ttgggccaga gcggtatcga
      781 taatggcgtt catccacccg gagtatgcat gatagggacc gtagttgggg tcgtagggtg
      841 cgccgaagta ctcccaggcc tcgaagtcga atccgcgcat ccaacacccg tcgagccgcg
      901 gatcgggatc cgcacattgg atggcgacca ggtagtcggc gatgccctcg aacgcctcgc
      961 ggcaagccgg gtcgccgatg gccttgtatg ccgcccaggc ggccagcgca tcccacgagg
     1021 tctggtagag ctggtcggtg agtggctcgc cggcaagggt gttgatggag cagtcattct
     1081 cgaacgccca ctcgtaggag ccgggtcctc ggaggcgcat gacaccgtaa cggcgcacgt
     1141 cgggatcagc caggtagcgg gcccagtagc cggccaggga ccgagcggca tccggggtac
     1201 ccaggatgcg agcggcgtgc gccatgccca aggagcccac cgagccgccg tggacttcgc
     1261 agacgcgtcg cgccagcgcc cggtagaccg ggtcacgcgt cgcgagcgcg gcaccggccc
     1321 agcgttggat gtcgaacgcc gggtagccgg cacccgaggc tcggtcccgc caggattgcc
     1381 tcccgatccg gtcaagctcc acggggtcga cccattgccc cgcaatgacg ccatcgtcct
     1441 cacgggcaag ggcccgggca agctcagtgt tgcgcagagc gggcatgagc caccgcctgt
     1501 cacccgtgcg ctcccagagc cagaggagga cgttcgcgat ccgcgtgttg tcgtcccagg
     1561 ggaagctggc gtcagcatgg acgtcgagcc acgggaagcc accgaagcgc ggcagggagg
     1621 gatcggagaa ctggtgaccg acgagaagct cagccatgcg aagggcacgg tcatgccagc
     1681 ggccgtctcc tgtgacgtcg ccgtagagcc acagcgcttt gagagtcatg gcgttgcagt
     1741 cgcagcgata ggacgaggcc agctcctcca cggggccgcc gaatagggcg gacagggcaa
     1801 gggcgctgtg cacgccgagc gagccgtcgg ctctgggcat gatgccactg cgcacgaacc
     1861 ataacagatt gcggtcgagg gcccgccggt aggcctctcg ccgttgcgag ggctcgatgt
     1921 gtatcgcggg cgtggacggc gggtcagggg ccggcactgt ggcccccggg accgtgcggg
     1981 tgactatggc acgccagagt tgggaccacg caaggga
//
LOCUS       DAOSPL010000948         2012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_56115_length_2012_cov_3.111906, whole genome shotgun sequence.
ACCESSION   DAOSPL010000948 DAOSPL010000000
VERSION     DAOSPL010000948.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56115_length_2012_cov_3.111906"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>439)
                     /locus_tag="PLQ54_15535"
     CDS             complement(<1..>439)
                     /locus_tag="PLQ54_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94731.1"
                     /translation="DFGRTLSRSRRVPASTFAEYRKRLDSQVGKLERALSVAQTQKAV
                     AETGKDQGYLIQIAHAMTKVFREQPPVGQPAGVVSIRSARNEYESFQLLLVAGAEALS
                     NVRVKASDLRKADGEAIDSDHIEIRRVGYVNVTAPTPGGGPAGW"
     assembly_gap    440..449
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<450..1140)
                     /locus_tag="PLQ54_15540"
     CDS             complement(<450..1140)
                     /locus_tag="PLQ54_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94732.1"
                     /translation="MRFRAALWLVGLMALVALCAVAPAQDDVQYISYSLSERDVWKAG
                     FFDPTGADAAGRVKLTVRRASAELQADFTGAGEGAYACVATGSPCAAKRRDLFRLETE
                     NAFVAEVRYRNLTSSSKLVLAWGRLGKGGGLGSKELPHRTEWGTAFVDITDPKLNGGR
                     SPNAIGSLHLHLIPGPNAGVERVEVESVKVHAAGVADYDGLTSAYRTTADEAIRKAAD
                     MPATQARAIERA"
     gene            complement(1251..>2012)
                     /locus_tag="PLQ54_15545"
     CDS             complement(1251..>2012)
                     /locus_tag="PLQ54_15545"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOS94733.1"
                     /translation="WLVSRSLMTNDQIMQEPHSLMDLIPAPVRLANYPDALAFKGFHF
                     TTYLTNTLIICALTVFGTILSCSLVAYGLSRIYWRGRNVVFYILLGTMMLPAQVTMIP
                     VFVVFAKLRLVDTFVPLILPAFLGNAFFVFLLRQFFLGIPRDLTDAARLDGCTEFGIW
                     RQVVIPLSKPALATVGLFSFMGAWNDYLGPLIYLSDSSKFTLSLGLASFSSQYGQEPG
                     MLMAATTVMTVPIIVLFFFAQRTFIQGITLTGLKG"
BASE COUNT          363 a          666 c          663 g          310 t
ORIGIN      
        1 accagccggc cggaccgccg cccggtgtgg gtgctgtcac gttcacatag ccgacccgcc
       61 ggatctcgat gtggtcgcta tcgatcgcct cgccgtcggc cttacgcagg tcagaagcct
      121 tgactcgaac gttcgacagc gcctcggccc ccgccacgag caggagctgg aaactctcat
      181 actcattgcg ggccgaccgg atggacacca caccggccgg ctggccgacg ggcggctgtt
      241 cgcggaacac cttggtcatg gcgtgcgcga tctggatgag gtacccctgg tccttgcccg
      301 tctcggccac cgccttctgc gtctgcgcca cactgagcgc gcgttcgagc ttgcccacct
      361 gggagtccaa ccgcttgcgg tactcagcaa aggtgctcgc cgggacgcgc cgcgatcgtg
      421 aaagtgtccg gccgaaatcn nnnnnnnnnc ggcccgctcg atggcccggg cttgggtggc
      481 tggcatgtcg gcggccttgc ggatcgcttc gtcagccgtc gtgcggtacg ccgacgtcag
      541 tccgtcatag tcggctaccc cggcggcgtg gaccttcacg ctctcgacct cgacccgctc
      601 gacgccggcg ttcgggccgg gtatgaggtg caggtgtagt gagccgatgg cgttggggct
      661 ccggccgccg ttgagcttgg gatcggtgat atccacgaag gcagtgcccc actcggtccg
      721 gtgagggagc tccttcgagc cgagaccgcc gcccttgccg aggcgccccc aggccagcac
      781 gagcttggat gacgatgtga ggttgcggta gcgcacctcg gccacgaacg cgttctcggt
      841 ctcgagccgg aacaggtcgc gccgcttggc ggcgcatggc gagccggtgg ccacgcaggc
      901 atacgcgccc tcgcccgctc cggtgaagtc cgcctgcagt tccgccgacg cgcggcgtac
      961 cgtgagcttc acccggcccg ccgcgtcggc gccggtcgga tcgaagaacc cggccttcca
     1021 gacatcgcgc tcggataggc tatagctgat gtactgcacg tcgtcttgcg cgggcgccac
     1081 ggcacacaga gccaccagcg ccatcagacc tacgagccac aaagctgcac ggaatcgcac
     1141 ggatcgggca cctccggagt gttctgagtc gcgacgccgc tcgccagcaa gaccctcacg
     1201 ccacgcagat taccacagcg gcggagaacc gggatggccc ctgacccctg ctaccccttc
     1261 agacccgtca gcgtgatgcc ctggatgaac gtccgctggg cgaagaagaa cagcacgatg
     1321 atgggcacgg tcatcaccgt cgtggccgcc atgagcatcc ccggctcctg gccatactga
     1381 ctcgagaagc tcgccaggcc gagggacaga gtgaacttgg acgagtccga gaggtagatg
     1441 agcgggccga ggtagtcatt ccaggcgccc atgaacgaga agagacccac ggtggccagc
     1501 gccggcttcg agagcgggat gaccacctgc cgccagatgc cgaactcggt gcagccatcg
     1561 agccgggccg cgtccgtgag gtcgcgcggg atcccgagga agaactgccg aagcaggaac
     1621 acgaagaacg cgttcccaag gaacgcgggc aggatcagcg ggacgaatgt atccaccagc
     1681 ctcagcttgg cgaacaccac gaagaccggg atcatcgtca cctgggcggg cagcatcatc
     1741 gtgcccagca ggatgtagaa cacgacgttg cggccacgcc agtagatgcg cgacagaccg
     1801 taggccacca gggagcatga caggatcgtg ccgaacacgg tcagggcgca gatgatcagc
     1861 gtgttggtca ggtacgtcgt gaagtggaag cccttgaatg ccagcgcgtc ggggtagttc
     1921 gccaggcgca ccggcgccgg gatcagatcc atcagcgagt gcggctcctg catgatctgg
     1981 tcgttggtca tcagcgaccg cgagaccagc ca
//
LOCUS       DAOSPL010000949         2012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_56121_length_2012_cov_2.779254, whole genome shotgun sequence.
ACCESSION   DAOSPL010000949 DAOSPL010000000
VERSION     DAOSPL010000949.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56121_length_2012_cov_2.779254"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..52)
                     /locus_tag="PLQ54_15550"
     CDS             complement(<1..52)
                     /locus_tag="PLQ54_15550"
                     /inference="COORDINATES: protein motif:HMM:NF012868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94734.1"
                     /translation="MVTGVGLLIHIYSMGYM"
     gene            complement(46..345)
                     /locus_tag="PLQ54_15555"
     CDS             complement(46..345)
                     /locus_tag="PLQ54_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94735.1"
                     /translation="MDFLWTKHIWLIPFWPALGALANGVLIPRARKTLDVVSTVAIGS
                     VAMSFLCTVFLTAELASLRIGNPAPVEYFRWIDAGDLRVPFALLFDPLSATMCLW"
     gene            complement(353..655)
                     /gene="nuoK"
                     /locus_tag="PLQ54_15560"
     CDS             complement(353..655)
                     /gene="nuoK"
                     /locus_tag="PLQ54_15560"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOS94736.1"
                     /translation="MVPLSWYVAVACVLFTIGVIGVLACRRTIVILMAIELMLNAGNL
                     LLVALAREFWSAQAQAIVLFVLAVAAAEVAVGLAIIVRMFELKGTLDIDEVNLMKW"
     gene            complement(655..1212)
                     /locus_tag="PLQ54_15565"
     CDS             complement(655..1212)
                     /locus_tag="PLQ54_15565"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOS94737.1"
                     /translation="MAWPHVTCFGISSALAITAAVMVVTRRNAVHAALWLAVALVSTG
                     VLYLTLSAQFLAVLQVIIYAGAIVVLFLFVVMLLNVTEDQATDRRRWLAVVGLALALV
                     WGAGTALLLLAGPPAGTGLVVRTPGDVALRGTAEELARAVFEHHALAFELVSLILLAA
                     MAGVIALARRTPRGAEEIPGKRGSP"
     gene            complement(1213..1746)
                     /gene="nuoI"
                     /locus_tag="PLQ54_15570"
     CDS             complement(1213..1746)
                     /gene="nuoI"
                     /locus_tag="PLQ54_15570"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HOS94738.1"
                     /translation="MAGEGARPGGGASWLARLWRATGGHAAVQIARGMLTTIKHVPKR
                     PVTVSYPEVKPELGSRWKGLHYLARYENGLERCVGCSLCALACPAHCITVVAAENRDD
                     ARFSPGERYARVYDIDELRCIFCGLCVLACPEDAIRMTRKYDFAYSTREQFVYHKEQL
                     LERAPGAAEDPSAWGYD"
     gene            complement(1747..>2012)
                     /locus_tag="PLQ54_15575"
     CDS             complement(1747..>2012)
                     /locus_tag="PLQ54_15575"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOS94739.1"
                     /translation="FGGWSGLGWFDLDRLPAWLGWYSTIAPTVIFFTKQMVFMVGAIW
                     VRATLPRMRYDQLMNLGWKVLIPVATANLVALALFEAASQRWF"
BASE COUNT          394 a          699 c          635 g          284 t
ORIGIN      
        1 ccatgtagcc catcgaatag atgtggatga gcagtcccac gcccgtcacc ataggcacat
       61 ggtcgccgac agcgggtcga acagcagggc gaagggcacc cgcagatcgc cggcatcgat
      121 ccagcggaag tactccaccg gagccggatt gccgatccgc agtgaggcca gttcagccgt
      181 aaggaacacg gtgcacagga acgacatggc gactgatccg atagccaccg tcgagaccac
      241 gtcgagcgtt tttcgcgccc gtgggatcag cacgccgttc gccagggccc ccagggcggg
      301 ccagaagggg atcagccaga tgtgcttagt ccacaggaag tccacggcga gcctaccact
      361 tcatcaggtt gacttcgtcg atgtccaggg tccccttgag ctcgaacatc ctcacgatga
      421 tcgccagccc cacagcgacc tcggccgccg ccaccgccag tacgaacagc acgatagcct
      481 gggcctgagc actccagaac tcgcgggcca gagccaccag cagcaggttg ccggcgttca
      541 gcatgagctc gatcgccatg aggatcacga tggtccggcg gcatgccagc acgccgatga
      601 cgccaatggt gaacaggaca cacgccacag ccacgtacca ggagaggggc accatcaggg
      661 actccccctc ttgcccggga tctcctcggc gccgcgcgga gtgcgccgcg ccagggcgat
      721 gacacctgcc atggcggcga gcaggatcag cgacacgagt tcgaaagcga gcgcatgatg
      781 ctcgaacacg gcccgcgcca gttcctcggc ggtgcccctc agtgccacgt cgccgggtgt
      841 gcggaccacc aggcccgtgc cggccggcgg gcccgcgagc agcagtaggg cggtgcctgc
      901 gccccatacc agagccagcg ccagtcccac aacagccagc cagcgccggc ggtcggtggc
      961 ctggtcctca gtcacgttca gtagcatgac gacgaagagg aacagcacca cgatggcccc
     1021 cgcatagatg atgacctgca gcaccgccag gaactgggca ctgagggtca ggtacagcac
     1081 gccggtggac accagggcca cagccagcca tagtgcggca tgcacggcgt tccggcgtgt
     1141 gacaaccatg acggctgcgg tgatcgccag cgccgagctg atgccgaagc aggtgacgtg
     1201 gggccaagcc aactagtcgt acccccaagc ggatggatct tcggcggctc ccggggctcg
     1261 ctcgagaagc tgctccttgt ggtacacgaa ctgctcacga gtgctgtagg cgaagtcgta
     1321 cttccgcgtc attcgtatcg cgtcctcggg gcatgccagc acgcacagcc cgcagaagat
     1381 gcaccgcagc tcgtcgatgt cgtacacccg cgcataccgc tcgccggggg agaagcgcgc
     1441 gtcgtcgcga ttctccgccg ccaccaccgt gatgcagtgg gcggggcagg ccaacgcgca
     1501 cagcgagcat cccacgcagc gttcgaggcc gttctcatac cgcgccaggt agtggagccc
     1561 tttccaccgg ctgcctagct cgggcttgac ttccgggtag gataccgtca ccggccgctt
     1621 cggcacgtgc ttgatggtgg tcagcatccc cctggcgatc tgcacggccg cgtggccgcc
     1681 agtggcgcgc cagagccggg ccagccacga cgcgcccccc ccgggccgtg ccccctcgcc
     1741 tgccatctag aaccacctct gggatgcggc ttcgaacagc gccagcgcga ccagattggc
     1801 ggtcgcgacg ggtatcagga ccttccagcc cagattcatg agctggtcgt accgcatccg
     1861 gggcagcgtc gcccggaccc agatggcccc gaccatgaac accatctgct ttgtgaagaa
     1921 gatcaccgtc ggagcgatgg tcgagtacca gccgagccag gccggaaggc gatccagatc
     1981 gaaccacccg agcccggacc acccgccgaa gt
//
LOCUS       DAOSPL010000950         2012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_56122_length_2012_cov_2.778232, whole genome shotgun sequence.
ACCESSION   DAOSPL010000950 DAOSPL010000000
VERSION     DAOSPL010000950.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56122_length_2012_cov_2.778232"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PLQ54_15580"
     CDS             <1..812
                     /locus_tag="PLQ54_15580"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOS94740.1"
                     /translation="NRATQGLYPPGSAFKILLAAAALDSGKITEGFEATCTGVEVIEH
                     DRIVCSSKQGHGRITMERALAGSCNIYFAHVGLELGPGLFTQYARAFGIGEALDIGVN
                     AAVSRLAAGGDLATTALLLESSFGQGQLQITPLQMAMIAGTIANRGVLMHPHVIGSVS
                     DGDEVIHRSAAQPMRQVVAPEVAGRVAEMMHAVTTSGSGRGGAVPGVPTAGKTGTAEN
                     PHGRPHAWFVGFAPVRAPRIAVAVVVENGGSGGSVAAPIARDVMRAWLQRN"
     gene            914..>2012
                     /locus_tag="PLQ54_15585"
     CDS             914..>2012
                     /locus_tag="PLQ54_15585"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOS94741.1"
                     /translation="MTGTLLCNERYEVLERIGDGGAAVVYRARDHSLNRDVAVKVLRD
                     ELAQDHEFVERFQREAHAAAGLSHPNIASVFDIGFEGGRHYFVMELLTGGTLRERLAR
                     ERKLSPEEALRIASQVAAALRHAHDSGIIHRDIKPQNILFTREGDVKVGDFGIARAMA
                     ASSVSQTGTIIGSVRYLAPEQAGGDGAVPQSDLYSLGIVLFEMLTGQVPFDAETPVAI
                     ALKHVQDPVPSVRAIDPTIPLAVEELLQRLLAKRVSERCPSAHQLITELYTVQQAVAV
                     QFGRAAAPAAASDDTTRVIRRPQPVRQAYQDEVVRRSIPPNPAAHSDQMSPVTWVLVF
                     LTAVVGIGAIVFLWLTRPPADHGGEKAPAMTT"
BASE COUNT          317 a          660 c          684 g          351 t
ORIGIN      
        1 tgaatcgggc gacacagggg ctgtacccgc cgggttcggc gttcaagatc ctcctcgccg
       61 ctgccgctct cgattctggt aagataaccg agggctttga ggccacgtgc acgggcgtcg
      121 aagtcattga gcacgaccgt atcgtgtgct cctcgaaaca ggggcacgga cgcatcacca
      181 tggagcgggc cctcgcgggc tcgtgcaaca tctacttcgc ccacgtgggt ctcgaactgg
      241 gcccgggctt gttcactcag tatgccaggg cgttcggcat cggcgaggcc ctcgatatcg
      301 gtgtgaatgc ggccgtgagc cgcctggcgg ccggcgggga tctcgcgacg acggccctgt
      361 tgctcgagag ctcattcggg cagggacagc tgcagatcac gccgctgcag atggcgatga
      421 tcgccgggac catcgccaat cgcggggtgc tgatgcatcc acacgtgatc ggatcggtat
      481 cggatggcga tgaggtgatc caccggtcgg cggcccagcc gatgcggcag gtggttgctc
      541 ccgaggtggc cgggcgggtg gcagagatga tgcacgcggt gacgacgtcg ggcagtggcc
      601 ggggtggcgc ggtgccgggg gtcccgacgg cgggcaagac cgggacggct gagaacccgc
      661 acggcaggcc tcatgcctgg ttcgtcgggt tcgctcccgt gcgggcgccg cgtatcgctg
      721 ttgcggttgt ggttgagaac ggtggctcgg gcggcagcgt ggccgcgccg atcgcgcggg
      781 atgttatgcg tgcctggctt caacgtaact agtccggcgg caccacgccg ctgcgggctg
      841 cgaggttcct cggcaccccc cgtgccagga ggccccgcag cgacgactgt gggccggagg
      901 tcggcggctg aacatgactg gtaccctgct ctgcaatgaa cgctacgagg tgctcgagcg
      961 cattggcgac ggcggagccg ccgtggtcta ccgggcccgc gatcactcgc tcaatcgtga
     1021 cgtggcggtc aaggtgctgc gcgatgagct ggcacaggat cacgagttcg tcgagcgctt
     1081 tcagcgagag gcgcacgcag ccgccggact gagccatccc aatatcgcca gtgtctttga
     1141 catcggtttt gagggtggtc gccactactt cgtgatggaa ctcctcaccg gagggaccct
     1201 tcgtgagcgg ctggcccgtg agcgcaagct ctcacccgag gaagctctcc gtatcgcctc
     1261 tcaggtggcc gctgccctgc ggcacgcgca cgatagtggc atcatccacc gcgacatcaa
     1321 gccacagaac atcctgttca cccgcgaggg cgacgtcaaa gtcggcgact tcggtatcgc
     1381 ccgagccatg gccgctagct cggtcagcca gacgggcacc atcatcggtt cggtgcgcta
     1441 tctagcgcct gagcaggccg ggggcgatgg cgcggtccct cagtcggacc tctactcact
     1501 cggcatcgtg ctgtttgaga tgctgaccgg ccaggtgccc ttcgatgccg agacccccgt
     1561 ggccatcgcg ctgaagcacg tgcaggatcc ggtgccctcg gtgcgggcga ttgaccccac
     1621 gattccactg gcggtcgaag agctgctcca gcggttgctc gccaagcgcg tctcggagcg
     1681 ttgcccgtcg gcgcaccagc tcatcaccga gctctacacg gtccagcagg ccgtggccgt
     1741 ccagttcggg cgtgccgcgg cacctgctgc ggccagcgac gacaccaccc gtgtgatccg
     1801 cagaccgcaa cccgtccggc aagcctatca ggacgaggtc gtccgccgct ccatcccgcc
     1861 caatccggcc gctcactccg accagatgtc tccggtcaca tgggtattgg tctttctcac
     1921 cgccgtggtc ggcatcggcg ccatcgtgtt cttgtggctc accagacccc cggccgacca
     1981 tggcggagag aaggccccag cgatgacgac cg
//
LOCUS       DAOSPL010000951         2011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_56158_length_2011_cov_2.930470, whole genome shotgun sequence.
ACCESSION   DAOSPL010000951 DAOSPL010000000
VERSION     DAOSPL010000951.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56158_length_2011_cov_2.930470"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLQ54_15590"
     CDS             complement(<1..345)
                     /locus_tag="PLQ54_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94742.1"
                     /translation="MRTKAPVALLTLIAALHPVASLAGGGSSLWTASSAAGFRERLGG
                     IALAPDAQAVADVLVAAGSAGLPIVADASLCDVRLDDLGVAAGASCAVVLEALYASER
                     GLLYFDHGILAVT"
     gene            complement(414..938)
                     /locus_tag="PLQ54_15595"
     CDS             complement(414..938)
                     /locus_tag="PLQ54_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94743.1"
                     /translation="MRTLQSLAWLFGCVAACGCIVVSSASADVACCFVIPYVEESASA
                     CAQYPEWNIPDIPGEYHGWYQDYPKPGWGLPIPVAFADEDICVQNFCAIFPQSTTGIY
                     FFNPTNDPWIDVPAGKTAICTGWGTRVCTHRETHWLFALMLGYPVPIPMGSCQECSTC
                     FIDLMTSHITVTVQ"
     gene            complement(960..>2011)
                     /locus_tag="PLQ54_15600"
     CDS             complement(960..>2011)
                     /locus_tag="PLQ54_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94744.1"
                     /translation="NYSGSRLRARVAVTMTLGVHYPVCDQGGKVVGYLRGELAGAMLA
                     GMGGELCQFAGVTGGDVWAAPRALEGERGARALQAIAEGCGGALPAADNVLPAELGLL
                     PDRHDEAAGRIASAPLLVTRPGGPCTFGEFLASLGSADRERCSCDPDIAGRPLWLAIG
                     GQPAARDVLEAACMAAGAHPREVMGLPHVGLDPDGVWRDHLCEPVHRDFMRRLAETAV
                     VPMLALGRLVDGSADGLQGLAPWPTALDPIKGTLSPEQCRSRVATPSSAKGPTAGEMH
                     EGLAAGQLVAFPVVRVDLAACHLLREAAPAGASADEARFIGTEFIPYRVATYGPAGYA
                     EPGTERLLSRPPIIP"
BASE COUNT          353 a          694 c          687 g          277 t
ORIGIN      
        1 ggtcacggcc agaatcccgt ggtcgaagta aagcaggcca cgctcactgg cgtatagcgc
       61 ctccagaacc acggcacacg aggcgccggc cgcgacgccg aggtcgtcca gccgcacgtc
      121 gcagaggctg gcatcggcga cgatcggaag acctgcactg ccagcggcca ccagcacgtc
      181 cgcgacggcc tgcgcgtcag gagcgagggc gatgccccca agccgctccc tgaatcccgc
      241 cgcagacgag gccgtccaga gcgacgagcc gccgcccgcg agtgaagcga cgggatggag
      301 cgcggcgatg agggtcagaa gcgcaaccgg cgccttggta cgcatggcga gttccccacc
      361 taacgcgcgc ggcgcgaccc ggcggccgac ggcgcacctt agttgacgga tggtcactgg
      421 acggtgacgg tgatgtggct ggtcattagg tctatgaaac aggtcgagca ctcctggcag
      481 gaccccatgg ggatgggaac cggatagccg agcatgagcg cgaagagcca gtgcgtctcc
      541 ctgtgggtgc agacccgcgt cccccagccg gtgcagatcg cggtcttgcc cgcggggaca
      601 tcgatccacg gatcattggt ggggttgaag aagtagatgc ccgtcgtgct ctgcgggaaa
      661 atggcgcaga agttctgcac gcagatgtcc tcgtcagcaa acgcgacggg gattggcagc
      721 ccccaaccgg gcttgggata gtcctggtac cagccgtggt actcgccagg gatgtccggt
      781 atgttccatt cggggtactg cgcgcaggct gaggcgctct cttccacgta agggatcacg
      841 aagcagcacg ccacgtcggc cgacgcggac gagacgacga tacagccgca tgctgcgacg
      901 cagccgaaca gccatgcgag gctctggagt gtgcgcaagc agtatcctcc ttcttgtcat
      961 caggggatga tcggcggcct ggacaacagc ctctccgttc cgggctcagc gtagccggca
     1021 ggaccgtacg tcgcgacccg gtatggaatg aactcggtgc ctatgaaccg ggcctcgtct
     1081 gcgctcgcgc ctgcgggggc cgcctcacgc agcaggtggc acgcggcaag gtcgaccctc
     1141 acgaccggga acgctacaag ctgcccggcg gccagcccct cgtgcatctc gccggccgtc
     1201 ggccccttgg cactcgaggg agtcgccacc cggctacggc actgctcggg gctgagggtc
     1261 cctttgatcg ggtcgagtgc ggtcggccaa ggagccaagc cctggaggcc gtccgccgac
     1321 ccgtcaacga ggcgtccgag ggctagcatc ggcacgactg ccgtctcggc gagccggcgc
     1381 ataaagtccc ggtgcacggg ttcgcacagg tgatcgcgcc agacgccgtc ggggtcgagc
     1441 ccgacgtgcg gcagacccat gacctcccgc gggtgtgcgc cagcggccat gcaggcggcc
     1501 tcaagaacgt ctcgcgctgc cggctggcca ccgatggcaa gccacaacgg gcggcccgcg
     1561 atgtcggggt cgcagctaca tcgctcgcga tctgccgatc cgagagacgc gaggaactcc
     1621 ccgaacgtac agggcccacc cgggcgcgtg accagtaagg gggcggacgc gattctacca
     1681 gcggcctcgt cgtggcggtc cggcagcagg cccagctcgg ccggcaacac gttgtcagcc
     1741 gccggcaagg cgccaccgca cccctccgcg atcgcctgaa gggctctcgc gccgcgctcg
     1801 ccctcaagcg cacggggcgc cgcccacacg tcgccgccgg tcacgccagc gaactggcac
     1861 agctcgcccc ccatgccggc tagcatggcg ccggccagct ccccgcggag gtaccccacg
     1921 accttgccgc cctggtcgca cactgggtag tgaacaccga gagtcatcgt gaccgcgact
     1981 cgcgcgcgca agcgagagcc gctgtagttg g
//
LOCUS       DAOSPL010000952         2010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_56180_length_2010_cov_3.369821, whole genome shotgun sequence.
ACCESSION   DAOSPL010000952 DAOSPL010000000
VERSION     DAOSPL010000952.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56180_length_2010_cov_3.369821"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLQ54_15605"
     CDS             <1..374
                     /locus_tag="PLQ54_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOS94745.1"
                     /translation="EIDGSAAQTLVNNGCIAVGEGANMPSTPEAVRIFLDNNVAFGPA
                     KAANAGGVATSSLEMCQNSQRLNWTFQEVDTRLQLIMDSIYEQCTQAAEEYGSPGNLV
                     VGANIAGFQKVARAMLAQGVA"
     gene            complement(471..>2010)
                     /locus_tag="PLQ54_15610"
     CDS             complement(471..>2010)
                     /locus_tag="PLQ54_15610"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOS94746.1"
                     /translation="ETFLDGAETARQRMLTGTFPDSIVSQFAQMLDYFGQCPIIVRSS
                     SLLEDNFGNAFAGKYDSVFCVNQGHRDQRLEDFLSAVRSIYASSMSERALRYRAQRGL
                     LDRDEQMALLVQRVSGAVRGDLFYPEMAGVGLSFNPYVWSEHIDPHAGVLRLVFGLGT
                     RAVDRSDDDYTRIVALNAPRRRPEAGFDQVRRYAQRRVDCLDLEANRLTSLALRDVLA
                     RSQGLAMDMFASRDEEAEERASTQGASAACPWVLTFDRLLSDTDYVETMREMLRVLEE
                     AYGYPVDVEFTTNFLDGSEYRIGLVQCRPLQVREGGAAAAPPTDVPRERLVLRAHGAV
                     IGHSAPLDVDRIIYVVPSAYGRLPTPDRYAVARLIGDITRSDVEARTVLLAGPGRWGT
                     STPSLGVPVTFSDIHTISALCEIVAMRDDLVPDVSLGTHFLNELIEMDILYLALFPGR
                     DGNWLNSDLLERAPSRLLDLIPDAGGWDTVVRVLDSRALGGRNLRLHADVLRQDAVCC
                     VDGG"
BASE COUNT          369 a          668 c          662 g          311 t
ORIGIN      
        1 acgagatcga cggctcggcc gcccagactc tggtcaacaa cggatgcatc gccgtcggtg
       61 aaggggccaa tatgccgtcc acgcccgagg cggtccgcat cttccttgac aacaacgtgg
      121 cctttggccc cgccaaggcc gccaatgcgg ggggcgtggc cacgagctcc ctcgagatgt
      181 gccagaactc ccaacgcctc aactggacct tccaggaagt cgacacgcgg ctccagctca
      241 tcatggacag catctacgag cagtgcacgc aggccgcaga ggaatacggc agtccgggca
      301 acctggtggt gggagccaac atcgcggggt tccagaaggt ggcgcgtgcc atgctcgcgc
      361 aaggcgtggc gtagtgcgtt cgtcccagtg ggagacgggt gacggctccg cagtacgccc
      421 gagcccgttg cccgtctcca atgcgcctcg ctgcggacgc gcccctcggc ttaacctccg
      481 tccacacagc acactgcgtc ctgcctcagc acgtcggcgt ggagcctgag attgcgcccg
      541 cccagggcac gggagtcgag cacccgaacc acagtgtccc acccacccgc gtccggaatg
      601 aggtccagca gacggctcgg ggccctctcc agaaggtcgc tgttcagcca gttcccatcg
      661 cggcccggga acagagccag gtagaggatg tccatctcga tgagctcgtt gaggaagtgg
      721 gtcccgagcg agacgtcggg gacaagatca tcccgcatgg ccacgatctc gcacagcgcc
      781 gatatggtgt ggatatcgct gaacgtgacc ggaactccaa gggatggtgt ggaggtgccc
      841 caccggccag ggccggcgag caacacggtg cgtgcctcga catcagaccg tgtgatgtca
      901 ccgatgagac gtgccaccgc atagcggtcg ggagtcggga gccgcccata cgcggacggg
      961 accacgtaga tgatccggtc gacgtccagg ggagcgctgt gcccgatcac cgcaccgtgg
     1021 gctcggagca caaggcgctc gcgcgggaca tcggtcggcg gagcggccgc ggccccacct
     1081 tcccgcacct ggagcggacg gcactgcacc aggccgatcc ggtactcgct tccgtccagg
     1141 aagttggtgg tgaactcgac gtcgaccggg tagccatagg cctcctcgag gacccggagc
     1201 atctcgcgca tggtctcgac gtagtccgtg tccgacagca gccggtcgaa ggtcagcacc
     1261 cacggacacg ccgcgctcgc gccctgggtg gacgcccgtt cctcggcctc ctcgtcgcgc
     1321 gaggcgaaca tgtccatggc cagcccctgg cttcgggcga gtacgtcccg cagggccaac
     1381 gaggtcagcc gattggcctc gaggtcgagg caatcgactc gacgctgggc gtagcggcgc
     1441 acttggtcga agccagcctc ggggcggcgc cgcggcgcgt tgagcgccac aatccgggtg
     1501 tagtcgtcat cggatcggtc cacggcccgg gtgcccagtc cgaacacgag acgcagtact
     1561 cccgcatgcg ggtcgatgtg ctcgctccag acatatgggt tgaaagacag cccgacgccg
     1621 gccatctcgg ggtagaagag gtctccgcgc acggcacccg agacgcgctg aaccagtagg
     1681 gccatctgtt cgtcgcgatc cagcagacct cgctgcgccc ggtaccgcag tgcgcgttcg
     1741 ctcatcgagc tggcatagat ggatcgcacg gccgatagga agtcctcgag ccgctggtcg
     1801 cgatgcccct ggttcacgca gaacacgctg tcgtacttcc ccgcgaacgc attgccgaag
     1861 ttgtcctcga gcagactgct ggatcgcaca atgatgggac actgcccaaa gtagtcgagc
     1921 atctgggcga actggctcac aatgctatcg gggaaagtgc ccgtgagcat gcgctggcgg
     1981 gcagtctcag cgccatcgag gaaggtctcg
//
LOCUS       DAOSPL010000953         2007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_56292_length_2007_cov_3.306352, whole genome shotgun sequence.
ACCESSION   DAOSPL010000953 DAOSPL010000000
VERSION     DAOSPL010000953.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56292_length_2007_cov_3.306352"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="PLQ54_15615"
     CDS             <1..1325
                     /locus_tag="PLQ54_15615"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOS94747.1"
                     /translation="RELVTATSFLFFAYIGFCTISQAGGEAKDPSRTLPRAFVWATVV
                     VTAYYLLFALALYHAVPWKYVWFQKSIAEAPLTAPGLLGILMPPILAGFVTLAAAITL
                     ANDVPPMLMAISRLFFSWAHDGVMPRGLARVNRRFRTPDVALTLCAAVACLVVVECHF
                     RPGSGFFKGVDTTTISLLFTYLVASVTVLALPRTNPELYRDVAFIRSRRAQVAVALLS
                     IATIGSLLAIQIRQDLSSLVRRVAIQQMAFPEDLEYHKSSQTWLRADGDDVVIGITPV
                     AWLGEPRVSGVQLPEVGRRLAARQPVATLLSSARGSSARPVMTPVAGEVIAVNRDLTH
                     NPGLCVTSPYDQGWLVRIRPEPSTEGPRAIPAAEYMASVGADVSTGRPAVSAALHAAT
                     RSAVVVWLVVLLVGAMVFRTMWARCRRAGIDPRAPFRALPDEGEETVE"
     gene            complement(1293..>2007)
                     /locus_tag="PLQ54_15620"
     CDS             complement(1293..>2007)
                     /locus_tag="PLQ54_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006921895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOS94748.1"
                     /translation="VVRALAGQAGTVISHAQLAAKLQEAQFETVFRLSMAAEYRDTDT
                     AAHIQRMSRYTEVIAQNLGLSARDVQLARLASPMHDVGKLGIPDAILLKPGKLTEEEW
                     HIMKSHTTMGARILSGSDSPVLQASEQVALTHHEKVDGTGYPVGLRGEEIPLLGRMAA
                     VADAFDAVTSRRCYKEARSLEEGLAVIRGDAGSHFDPECAEALERGFEQVKRIYELFA
                     DPEEALAPRSFNGLLPLVG"
BASE COUNT          278 a          676 c          664 g          389 t
ORIGIN      
        1 tgcgggagct ggtcaccgcc acgtcgtttc tgtttttcgc ctacatcggt ttctgcacca
       61 tctcgcaggc cgggggcgag gccaaggacc cctcccggac actgccacga gctttcgtct
      121 gggcgaccgt ggtcgtgacc gcctactacc tgctcttcgc gctggccctc taccacgcgg
      181 tgccatggaa gtatgtgtgg ttccagaaga gcatcgccga agcgcctctc acggcccccg
      241 ggcttctcgg gattctgatg cccccgatcc tcgccgggtt cgtgaccctg gccgcggcca
      301 tcacgctggc caacgatgtc ccccccatgc tcatggccat ctcgcgtctg ttcttctcgt
      361 gggcccacga tggggtcatg ccacgcggtc tggcgcgcgt gaaccgtcgg ttccgcacgc
      421 ccgatgtggc tcttactttg tgcgccgccg tggcatgtct tgtggttgtt gagtgtcatt
      481 tccgtcccgg cagcggcttc ttcaaagggg tggacacgac caccatttcg ttgctgttta
      541 cttacctcgt cgcatcggta accgtgctgg ctttgccgcg gacgaacccc gagctgtacc
      601 gcgatgtggc gttcatccgg tcgcggcgcg cacaggtggc cgtggcgctg ctctcgatcg
      661 ccaccatcgg cagcctgctg gcgatccaga tccggcagga tctatcgtca ctcgtgcggc
      721 gcgtcgcgat ccagcagatg gcgttccccg aggacctcga gtaccacaag agcagtcaaa
      781 cctggctccg ggccgatggc gacgatgtgg tgatcggcat cacgcccgtc gcgtggctcg
      841 gtgagccgcg cgtctcgggc gttcagctgc ccgaggtggg ccggaggctg gccgcccggc
      901 aacccgtcgc caccctgctg tcctcggcgc gcggctcgag cgcccgtccg gtgatgacgc
      961 cggtggctgg tgaggtcatt gctgtcaatc gcgacctaac ccataatccc ggtctctgcg
     1021 tcaccagccc ctatgaccag ggatggttgg tgcgcattcg gcccgagccg tcgacggagg
     1081 gccctcgagc catccccgca gccgagtaca tggccagcgt cggggccgat gtgagcacgg
     1141 gacggcctgc agtctcggca gcgcttcatg cggccacccg tagtgcggtg gtcgtgtggc
     1201 tggtggtgct gctggtgggc gcgatggtct tccggaccat gtgggcgcgc tgccgccggg
     1261 cgggcatcga tccgcgcgcc ccgttccgcg ccctacccga cgagggggag gagaccgttg
     1321 aatgagcggg gcgccagtgc ttcctcgggg tcggcaaata gctcgtagat ccgcttcacc
     1381 tgctcgaacc cccgttcgag cgcctcggcg cattccgggt cgaagtgcga accggcatcg
     1441 ccgcggatga cggccaagcc ctcctcgagc gatcgggcct ccttgtagca acggcgactg
     1501 gtgacggcgt caaatgcgtc tgccacagcg gccatgcggc ccagaagcgg gatctcctcg
     1561 ccgcgcagcc ccacggggta acccgtaccg tccaccttct catggtgtgt cagggccact
     1621 tgctcggagg cctgaagcac gggcgagtcg gaaccactca ggatccgggc tcccatggtg
     1681 gtgtgcgact tcatgatgtg ccactcctcc tcggtcagct tgccgggctt gaggagtatg
     1741 gcatcaggga tgcccaactt gcccacatcg tgcatgggac tggcgagacg ggcgagctga
     1801 acgtcgcgcg cgctgaggcc caggttctgc gcgatcacct ccgtgtaccg gctcatccgc
     1861 tggatgtgtg ccgctgtgtc ggtgtcgcgg tactccgcgg ccatggagag ccggaacacg
     1921 gtctcgaact gggcctcttg gagcttggcg gccagttggg cgtggctgat gacggtgccc
     1981 gcctgcccgg ccagagcgcg caccacg
//
LOCUS       DAOSPL010000954         2005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_56359_length_2005_cov_3.150769, whole genome shotgun sequence.
ACCESSION   DAOSPL010000954 DAOSPL010000000
VERSION     DAOSPL010000954.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56359_length_2005_cov_3.150769"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2005)
                     /locus_tag="PLQ54_15625"
     CDS             complement(<1..>2005)
                     /locus_tag="PLQ54_15625"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOS94749.1"
                     /translation="MLSAPPGGNAFWVQFEYPAPFTARSVTVAQLDAQSGQRGVLEAS
                     DDGTTWREVCEVAIPTPEITPVAGSITFEPARARFFRLSFVRRGARAARVALAELSLS
                     GGARLRGWAEKSGYTRQNDFAPDATPYDSGLCIAHEGVLDLTHRLAEDGTLNWEVPAG
                     DWTILRIGSTTTGKENHPATEAGRGLECDKLSREAVDLLFGGVLAPIMDDIGPLAGKG
                     LHQILVDSYEVGSQNWTPEFPEEFRKRRGYDPLPFLPAMAGRIVDSTEVTERFLWDVR
                     RTIADLYADNYWGRFAELCHAHNLKLATEPYGNGNFDDMQAGGRADIVMTEFWSGSGG
                     DYYGAKQVSSIAHTYGTGIVGAEAFTADSTNGKWLNHPASIKALGDAEYAAGVNRFIF
                     HRYAHQPWDHLVPGMTMGPWGMHFERTVTFWDQSTAWLRYLARCQYLLQAGLFHADVC
                     YYKGENAPSSLPGLSLPAGYDYDGCDTEVLLNRMSVRKGRIVLPDGMSYRLLVLHPSE
                     TMSPRVLARLRDLVRDGATLVGPRPSRSPSLEGYPACDVQVRETADEVWGDCDGQNVM
                     KHPYGRGVVYWGMPIEQVLAECGLAPDFEATADGTAPHLRWIHRTAGDTDYYFVSNQK
                     PLGEVVECTFRVSGRVPELWHPDTGRCERAPLYRVNGGRTTVTL"
BASE COUNT          329 a          672 c          671 g          333 t
ORIGIN      
        1 gcagggtcac cgtggttcgg ccgccgttga cgcggtagag cggggcgcgt tcgcaccggc
       61 cggtgtcggg gtgccagagc tcgggcacac ggccactgac tcggaaagtg cactccacca
      121 cctcgcccaa gggcttctgg ttggacacga agtagtagtc ggtatccccc gccgtccgat
      181 ggatccatct caggtggggg gcggtgccat cggcggtggc ctcgaagtcg ggcgccaggc
      241 cgcactcggc gagtacctgc tcgatcggca tgccccagta gaccacgcct cggccgtatg
      301 ggtgcttcat cacattctgc ccatcgcagt cgccccacac ctcgtcggcc gtctcccgca
      361 cctggacatc acacgcggga tagccctcga gactgggcga ccgactcggt cgtggaccca
      421 caagggtggc gccgtcgcga accagatcgc gcaaccgggc gagcaccctt gggctcatcg
      481 tctccgaggg atgcaggacc agcagccggt agctcatgcc gtcgggcaga acgatgcggc
      541 ccttgcggac ggacatgcgg ttgaggagca cctcggtgtc gcacccgtcg tagtcgtagc
      601 cggcgggaag cgagaggccc ggcagactcg agggcgcatt ctcgcccttg tagtagcaca
      661 cgtcggcgtg gaacaggccc gcctgaagca gatactggca ccgtgccaag tacctgagcc
      721 acgcggtcga ctggtcccaa aacgtcacgg tgcgttcgaa gtgcatcccc cacggcccca
      781 tggtcatacc cgggacaagg tgatcccacg gctgatgggc gtaccggtgg aagatgaacc
      841 ggttcacacc agcggcgtac tcggcatcgc ccagggcctt gatcgacgcc ggatggttca
      901 gccacttccc gttcgtgctg tccgcagtga atgcctcggc gccgacgatg cccgtgccgt
      961 aggtgtgagc gatggatgag acctgcttgg caccgtagta gtcgccgccg ctacccgacc
     1021 agaactcggt catcacgata tcggcccggc cgcccgcctg catgtcatcg aagttgccgt
     1081 tgccataggg ttccgtcgcc agtttcaggt tgtgcgcgtg gcagagctcc gcgaaacggc
     1141 cccagtagtt gtcggcatac aggtcggcga tggtccgtcg gacgtcccac aggaaccgct
     1201 cggtgacctc ggtgctgtcg acgatgcgcc cagccatggc aggcagaaag gggagtggat
     1261 catagccgcg cctcttccga aactcctcgg gaaactccgg agtccagttc tgcgagccca
     1321 cttcgtagct gtcgacgagg atctggtgga gccccttccc ggccagcggg ccgatgtcgt
     1381 ccatgatcgg ggccagcacg ccgccgaaca gcagatcgac agcctcgcgg ctgagcttgt
     1441 cgcactcgag ccctcggccg gcctcggtcg cgggatggtt ctccttcccc gtggtggttg
     1501 acccgatccg caggatggtc cagtcgcccg cgggcacctc ccagttgagc gtgccgtcct
     1561 cggctagcct gtgcgtgagg tcgaggacgc cttcgtgcgc gatgcacagg ccactgtcgt
     1621 agggggtcgc gtccggcgcg aagtcgttct gccgcgtgta tcccgacttc tcggcccagc
     1681 cgcgcagacg ggctccgccg ctcaatgaaa gctcggccag cgccacgcgg gcggcccggg
     1741 cgccgcgtcg gacgaacgag agccggaaga agcgcgcgcg ggccggctcg aaggtgatgc
     1801 tacccgcaac gggcgtgatc tcaggcgtgg ggatagcgac ttcgcagact tcacgccacg
     1861 tcgtgccgtc gtcggaggcc tcgagaacgc ccctctgtcc ggactgggcg tcgagctgcg
     1921 ccaccgtgac ggatcgtgcc gtgaatggcg ccgggtactc gaactgcacc cagaacgcgt
     1981 tcccgcctgg cggcgcagag agcat
//
LOCUS       DAOSPL010000955         2004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_56387_length_2004_cov_3.503335, whole genome shotgun sequence.
ACCESSION   DAOSPL010000955 DAOSPL010000000
VERSION     DAOSPL010000955.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56387_length_2004_cov_3.503335"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PLQ54_15630"
     CDS             <1..741
                     /locus_tag="PLQ54_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94750.1"
                     /translation="RNIALIQAPWMDAPVQLRFPETLNAGADLLYIDHTRADMPGRTD
                     TATLPGWHEGPGRAYWFDWPLYGGHRAGGTLSPRGDVVELEFWFRNAGTEPTTVLTQF
                     CLVQGNGALRDATGERSFIRSGGKWLRMADGDRGLGDPALVDYATPGGQDLRHMAVKS
                     DTWGLSTDPADCGLVAVQYPDGRHVVGLAFHRPRYILSNWHIPCMHADPTWSVCGPGD
                     RVSVHGRVYLLEGTLDDLWSRYCSDFPQ"
     gene            945..1076
                     /locus_tag="PLQ54_15635"
     CDS             945..1076
                     /locus_tag="PLQ54_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94751.1"
                     /translation="MDRLLMDRLDQANLRLHEPVWGAPRDCGCGGGAVTSEERTVRN"
     gene            1079..>2004
                     /locus_tag="PLQ54_15640"
     CDS             1079..>2004
                     /locus_tag="PLQ54_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94752.1"
                     /translation="MSTSTNHRGEGERLPRPESPSVRRLLWLAWPITGLLVALAIAYA
                     ILTSRHGVRTDRGWIDSRRATLPLPTKPVVADNGFHTYMAAVRSMWSVGNALPVPPGK
                     EYVWPPTLDDHELSRRTWEDVLEDADLGPGDMQCIARLVRANSRALQLLHEAADKAYV
                     SPREPDAVAYVDHCGQFRRLAQLGMAQVRVAARDGHDADALATARDVLALGANMPQYG
                     GLMEAAFGDMAIAVIHRHAMDVIQSRRLSRAEYLEHARYVRRLRECWYPLRQSAALDF
                     HVIKAMAEQADRSGEATLARLRGESCDAKTAAR"
BASE COUNT          306 a          695 c          682 g          321 t
ORIGIN      
        1 cgcaacatcg ccctcatcca ggccccctgg atggacgcgc ccgtgcagct tcgtttcccc
       61 gagacgctca atgccggcgc ggatctcctc tacatcgacc acactcgggc cgacatgccg
      121 ggccggactg acacggcgac gctgcccggc tggcacgagg gtccggggag ggcttactgg
      181 ttcgactggc ccctgtacgg ggggcaccgg gcgggaggca ctctcagccc acggggagac
      241 gtggtggagc tcgagttctg gttccgcaat gccgggacgg agcccaccac cgttctgacc
      301 cagttctgcc tggttcaggg caatggtgcc ctgcgcgatg ccaccggcga gcggtcgttc
      361 atccgctcgg gcggcaagtg gcttcgcatg gccgacggcg accgcggcct gggcgacccc
      421 gctctcgtgg actatgccac tcccggtggc caggacctgc gccacatggc ggtgaagtcc
      481 gacacgtggg gccttagcac tgaccccgct gactgtggcc tcgtcgcggt gcagtaccct
      541 gatggccgcc atgtggtggg tctggctttc cacagaccga ggtacatcct gagcaactgg
      601 catatcccct gcatgcatgc cgatcccacg tggtccgtgt gtggcccggg cgaccgggtg
      661 agtgtgcacg gcagggtcta cctcctcgaa ggtaccctgg atgacctatg gagccgctac
      721 tgcagcgact tcccgcagtg atgccggcgg ccgcgcgatg cggtggtccc cgcgtctgcc
      781 tgcggctgtc gtgccgtgcg cgcggagacg ttggagacgg agctgatcgg gatgtacgcc
      841 ccctgcatca cgggcctgac gctgatgtgc gcgctcgcct cgcccctcac ggaggctgat
      901 cccatggacg ccccatcgag atcagcgacc gccgggagct gttcgtggac cgactgctga
      961 tggaccggct ggaccaggcc aacctgaggc tgcacgagcc ggtctggggc gccccgcgcg
     1021 actgcggctg cggaggcggc gccgtaacga gcgaggaacg cacggtgagg aactgagcat
     1081 gagcaccagt accaaccatc gcggcgaggg cgagaggctg ccgaggcccg agagcccttc
     1141 ggttcgccgg ctgctctggc tggcttggcc catcaccggg ctccttgtgg ctctggccat
     1201 cgcctatgcc attctgacca gccggcacgg cgtccgcacc gaccgaggat ggatcgacag
     1261 ccgtcgagca accctccctc tgccgacgaa gccagtggtg gccgacaacg gctttcacac
     1321 ctacatggcg gcagttcggt cgatgtggtc ggtgggcaac gcgctgcccg tgccccccgg
     1381 taaggagtac gtgtggccgc cgaccctcga cgaccacgag ctctcgcggc gcacctggga
     1441 agacgttctc gaggacgccg acctcgggcc gggcgacatg cagtgcattg ctcgactcgt
     1501 tcgagcgaac tcgcgggcgc tccaactgct ccacgaggcc gcggacaagg cctacgtatc
     1561 gccccgtgag cccgatgcgg tggcctacgt cgaccactgc ggccagttcc gcaggctcgc
     1621 ccaactcggc atggctcagg tgcgcgtcgc ggcccgtgat ggccacgacg ccgatgccct
     1681 ggccacggcc cgcgacgttc tggcgctcgg cgccaacatg cctcagtacg gcgggctgat
     1741 ggaggccgcc ttcggcgaca tggccatcgc cgtcatccat cggcacgcga tggacgtgat
     1801 ccagagccgt cgcctgagcc gggccgagta cctcgagcac gcgcggtacg ttcgtcgtct
     1861 ccgggagtgc tggtaccccc tgaggcagtc ggcggcgctg gacttccacg tcatcaaggc
     1921 catggcagag caagctgacc gcagcggcga agccacattg gcacggctcc gcggcgagtc
     1981 ctgtgacgcg aagacggccg ctcg
//
LOCUS       DAOSPL010000956         2004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_56400_length_2004_cov_2.576706, whole genome shotgun sequence.
ACCESSION   DAOSPL010000956 DAOSPL010000000
VERSION     DAOSPL010000956.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56400_length_2004_cov_2.576706"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="PLQ54_15645"
     CDS             <1..1140
                     /locus_tag="PLQ54_15645"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOS94753.1"
                     /translation="GVRTASAHALWRAGEPGWSKHVHGNDEDIRALAGLRDARAVPAL
                     ARALLRARWGCAADAARGLGRFGGPRAVDALIAGLRRPEPFVVGAIAVALEALGDARA
                     AQPLLSALARPELDDAVPAIAKALGRVGGSAAVVPLLGVLSHAKPAARAAAAEALGAL
                     RDPRARDALADLVAREEGTNYHGATPWQAGARALGALADPRAIRPLLGLAPTDVSARD
                     ALRGLGLGDWVCGLTGAPGDLVRHGGEDNPWGFDLLQSALRHWGREARLYAAEELGRR
                     LDPQTVGELVTALGDSLEVRRAAAEALAAILQAHPSALDRQTWERVADKCRQPAVHRD
                     GPSYSHYDDPGGWDRDRLSKTIPSHGDHCDGIGVDFPDPPEGTDF"
     gene            1482..1976
                     /locus_tag="PLQ54_15650"
     CDS             1482..1976
                     /locus_tag="PLQ54_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94754.1"
                     /translation="MRMEVRCPCCVRLLSIPGNQAGKLGACCWCGQPLVAPRAQGDEP
                     TLPPPVAASGEPDAHLEPSYRNPTDGTEMILIPAGKAVFGDSRSCRFEAELPAYHLGR
                     FCVTITQYVAFLNSVRMGRGWRFEYGVSRQYGHLVERGRRFAVEDPGRYGDHPALMTF
                     ASLD"
BASE COUNT          292 a          674 c          729 g          309 t
ORIGIN      
        1 ggcgtgcgaa cggcgtctgc ccacgccctc tggcgggctg gcgagcccgg ttggtccaag
       61 cacgtccacg ggaatgacga ggacatccga gctctggctg gcctgaggga tgcgagggcg
      121 gtgcccgccc tcgccagggc tctgctcaga gcccgctggg ggtgcgcggc ggatgccgcc
      181 aggggactgg ggaggttcgg aggcccgcgc gccgtggatg ccctgatcgc cggactccgc
      241 cgcccggagc cgtttgtcgt tggggccatc gcagtcgccc tggaggcact gggggacgcc
      301 cgagccgcgc agccgctcct cagcgctctc gcccggccag agctggacga cgcggtccct
      361 gccatcgcga aggctctggg ccgcgtgggc gggagcgcgg ccgtcgtccc gctccttggc
      421 gtgctctccc acgccaagcc ggccgcccgt gccgctgccg cggaagcact cggcgcgctg
      481 cgcgatccgc gagcgcgtga cgcgctggcg gacctggttg cgcgcgagga gggtacgaac
      541 taccatggtg cgacgccctg gcaagcgggc gcccgcgccc tcggggcgtt ggccgatccc
      601 cgcgccattc gtccactact cggcctcgcc cccacagacg tgtccgctcg agatgccctg
      661 cggggcctcg gcctcggcga ctgggtctgt ggcctcacgg gcgcgcccgg cgatctcgtc
      721 cggcacggag gagaggacaa cccgtggggt ttcgacctct tgcagagcgc tctccgccac
      781 tggggtcgtg aggcccggct gtacgccgcc gaggagctgg gcaggcggtt ggacccacag
      841 accgtcggtg agcttgtgac agccctaggc gacagcctgg aggtcagacg ggcggccgct
      901 gaagctctgg ccgccatcct gcaggcccac ccatcggccc tggaccggca gacctgggag
      961 cgagtggcgg acaagtgccg gcagccagca gttcacaggg acggcccctc gtatagccac
     1021 tacgacgacc cggggggatg ggatcgggac aggctgtcga agacgatccc ctcgcacggg
     1081 gatcattgtg atggcatcgg cgtcgacttc cctgacccgc cagaggggac agacttctga
     1141 agcccgtgcg gctggtggac ggaccggttg tgggtcgcga ccgcgggcga cgcgctgtgg
     1201 cgaacgtcca gcgatgcagg tcatccccgc cgccactgga accgagcgga ggagtagtcg
     1261 gctccccgtg ctgacctccg gacggcgggg cccccagggc gaccatcgct gccggaccgc
     1321 cgtgaggagg cgaccgcatg ccagggccta gccgcgatgc cttcgacttc cgcgcagcct
     1381 acaagcatga cttcgcgtag ggaggtggcg tgcgctgcct tgagggcgcc tgccatcgcc
     1441 aggcgccatg tgtaccccgg aacctgtagg aggtctggcc gatgcgcatg gaagtccgct
     1501 gcccatgctg tgtccgtctg ctgagcatac ccggcaacca ggctggcaag ctaggggcct
     1561 gctgctggtg tggacagccg ctcgtggcgc cccgcgctca gggagacgaa cccacgctcc
     1621 cgccacccgt tgcggcgtca ggtgagccgg acgcacacct cgagccaagc taccggaacc
     1681 cgaccgacgg caccgagatg atactcatcc cggcagggaa ggcagtgttt ggtgactccc
     1741 ggagctgccg gttcgaggcc gagctaccag cctaccacct gggacgcttc tgcgtgacga
     1801 tcacgcagta tgtcgcattc ctgaactcgg tgcggatggg tcggggctgg aggttcgagt
     1861 acggtgtatc gaggcagtac ggtcacttgg tcgagcgcgg tcggcggttc gcggtggagg
     1921 accccgggcg ctacggggat catccggccc taatgacctt tgcgtccttg gattgaaggt
     1981 acggaatggg tttgtctggt acat
//
LOCUS       DAOSPL010000957         2001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_56497_length_2001_cov_3.241521, whole genome shotgun sequence.
ACCESSION   DAOSPL010000957 DAOSPL010000000
VERSION     DAOSPL010000957.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56497_length_2001_cov_3.241521"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /gene="sixA"
                     /locus_tag="PLQ54_15655"
     CDS             complement(<1..346)
                     /gene="sixA"
                     /locus_tag="PLQ54_15655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HOS94755.1"
                     /translation="MDLYFLRHGDAEPAEAGMTDAERALTRKGQKTTRAEAEGMARLG
                     VRPEVILTSPLVRARQTADIVAEVLGTGPPIETELLACGCRLGELQQALAPHMGRSSV
                     MVVGHEPDFGLLV"
     gene            complement(380..1450)
                     /locus_tag="PLQ54_15660"
     CDS             complement(380..1450)
                     /locus_tag="PLQ54_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOS94756.1"
                     /translation="MPHDDWASGLAFGLSRTPFLIDGKTRSICAENPNGAKGGGAREV
                     PERGVGAAGDLGAGWKVRPCIDLPAGSTTTLAAIDGPGVIQHIWITATPKSYRECVLR
                     MYWDGEDTPSVEVPLGDFFCNGHARRTNIVSLPINVNPSGGFNSYWPMPFRKSAKITI
                     ENQRWEPIGGFFYQVNYCLTEIPECAAYFHAQWRRSVTPREAPDHVILDGVRGKGQYV
                     GTYLAWTQLADGWWGEGEVKFFIDGDREFPTICGTGTEDYVGGAWCFDGQTYTAPFLG
                     YPMYEREPGAVPKHGLYRWHIMDPIRFDEDLRVTIQALGWWPNGKYQPLADDIASVAY
                     WYQMPPHAPFPALPSVQERFSR"
     gene            complement(1555..>2001)
                     /locus_tag="PLQ54_15665"
     CDS             complement(1555..>2001)
                     /locus_tag="PLQ54_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94757.1"
                     /translation="TEADEAEAQSLTAEAKLHLASVQLAQTDRRNASKTAHDAIEIAG
                     RLQARHTLWQGYHVLARIAQSKGQHHDASENYRQAIAVIEGIWWPLSSIGLAEPRDVK
                     PAVLNVYFDALRLAADHGEDEAVNRILSLSPWPFLRQRWEERIGSS"
BASE COUNT          300 a          675 c          676 g          350 t
ORIGIN      
        1 ccaccagcag gccgaagtcg ggctcgtggc cgaccaccat caccgaggac cgccccatgt
       61 gcggcgccaa ggcctgctgg agctctccga gccggcaccc gcaggccagg agttcggttt
      121 cgatcggcgg acccgtgccg agcacctcgg ccacgatgtc cgcggtctgc cgcgcgcgga
      181 ccagcggact ggtgaggatc acctcgggcc ggacgcccag gcgagccatc ccttcggcct
      241 cggctcgagt ggttttctgg cccttgcgtg tgagcgcgcg ttcggcgtcc gtcatacccg
      301 cttcggcggg ctccgcgtcg ccgtgccgca ggaagtagag gtccatgtga gtccctccac
      361 ctgtccgcgc ttcggcctgc tatcggctga accgctcctg gacgctgggc agcgccggga
      421 acggcgcgtg tggtggcatc tggtaccagt aggccaccga cgcgatatcg tcggccagtg
      481 gctgatactt cccgttgggc caccagccga gggcctggat ggtgaccctg agatcctcat
      541 cgaaccggat tgggtccatg atgtgccagc ggtacaggcc gtgtttcggg acggcgcccg
      601 gctcccgctc gtacatcgga tacccgagga agggcgccgt gtacgtctgc ccatcgaagc
      661 accacgcgcc tccgacgtaa tcctcggtgc ccgtgccgca gatcgtcggg aactcacgat
      721 cgccatcgat gaagaacttc acctcgccct cgccccacca cccgtcggca agctgtgtcc
      781 acgcgagata cgtgcccacg tactgcccct tgccgcgaac gccgtccagg atcacgtggt
      841 cgggggcctc ccgcggcgtg accgaacgtc gccattgggc atggaagtac gcggcacatt
      901 ccggaatctc ggtcaggcag tagttcacct ggtagaagaa ccctccgatg ggctcccatc
      961 gctggttctc gatggtgatc ttggccgact tccgaaacgg catgggccag taggagttga
     1021 agccgccgct tgggttcaca ttgatgggga gggacacgat gttcgttcgc cgagcgtgac
     1081 cgttgcagaa gaagtccccc agcggcacct cgacgctcgg cgtgtcctcg ccatcccagt
     1141 acatccgcaa gacgcactcg cggtaggact tgggggtggc cgtgatccag atgtgctgga
     1201 tcacgcccgg accatcgatg gccgcgaggg tcgtcgtcga gcccgccggc aggtcgatgc
     1261 atgggcgaac cttccatccg gcgcccagat cgcccgcggc tcccacgccg cgttcgggca
     1321 cctcgcgggc tccgcctccc ttggcgccgt tggggttctc cgcacagatg gagcgcgtct
     1381 tgccgtcgat caggaagggc gtccgcgaga gcccaaaggc caggccgctc gcccagtcgt
     1441 cgtggggcat ggtgacatcg ccgcctcgtc cgcagtgtgg ccgtgcgtgg aacgcattca
     1501 ggcttcgctg gaaccggcgg accctcctgc gccgggcggc cgggcggcgg cccgctagct
     1561 cgaaccgatc cgctcctccc agcgctggcg caggaagggc catggcgaga gcgacaggat
     1621 ccggttcacg gcctcgtctt cgccatggtc agcggcgagg cggagggcgt cgaagtagac
     1681 gttcagcact gcgggcttca catcgcgtgg ttccgccaag ccgatggatg acagcggcca
     1741 ccagatgccc tcgatcaccg cgatagcctg gcggtagttc tcgctggcgt cgtggtgctg
     1801 gcccttggac tgggcgatac gtgccagcac atggtagccc tgccaaaggg tatgccgagc
     1861 ctggaggcga cctgcgatct cgatggcgtc gtgggcggtc ttggaggcgt tccggcgatc
     1921 ggtctgcgcg agctgtaccg aggccaggtg gagcttcgcc tcggcggtca ggctttgggc
     1981 ctcggcctcg tcggcttcgg t
//
LOCUS       DAOSPL010000958         1998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_56617_length_1998_cov_3.134328, whole genome shotgun sequence.
ACCESSION   DAOSPL010000958 DAOSPL010000000
VERSION     DAOSPL010000958.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56617_length_1998_cov_3.134328"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1998
                     /locus_tag="PLQ54_15670"
     CDS             <1..>1998
                     /locus_tag="PLQ54_15670"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOS94758.1"
                     /translation="PASAQDDLAQGFQQPPPSARPWVYWFWLNGNITREGITADLEAM
                     ARVGIGGVLIMEVDQGAPVGPVPFAGAEWRELFRFVVSEAHRLGLEVNMNNDAGWNGS
                     GGPWVTPDKAMQKVVWTETDVEGPQRFEGVLAQPETVAGYYQDITVLAFPAVGDYRIA
                     DINGKSALVRQDRDQPAEFPDVPAGQTINHERIVKLADKMAADGKLTWDVPAGRWTIL
                     RLGHTCTGAMNAPAPESGRGLECDKLSKEGAEANFNGFMAHLIRDVGERAGDTLVATH
                     VDSWENGSQNWTPRFREAFKELRGYDLIDYLPAITGRVVDSLEVSERFLWDWRQTVSD
                     LLVENYAGHIAELAHRAGMRFTIEAYGDTTVDNLAYAGRADEPMGEFWSWPSFGAVGT
                     LTEMASAAHVYGKPIVGAEAFTAGDGEKWLYHPGWIKSMGDRAFCMGINRFVFHRYAL
                     QPWKDRRPGMSMGPWGLHYERTQTWWEQSGPYHEYLTRCQYLLQQGLPVVDVLYLAPE
                     GAPRDYNAPPSTHRTGYKGDACPAEVVLERLRVEDGRLVLPDGMSYAALSLPGAATMT
                     PKLLDRLAKLARDGATIVGTPPRKAPGLTDYPRCDAEVRRGARALWESGKVVTGKTAE
                     EVLAARGVAPDFSSDRALDFIHKRIGEADVYFVCNPSKHTVN"
BASE COUNT          341 a          646 c          703 g          308 t
ORIGIN      
        1 cctgcgagcg cacaggacga cctcgcccaa ggcttccagc agcccccgcc gtcggcccgg
       61 ccatgggtct actggttctg gctcaatggc aacatcacgc gtgaggggat cacggccgac
      121 ctcgaggcaa tggcgcgcgt gggcatcggc ggcgtgctca tcatggaggt cgaccaaggc
      181 gcccccgtgg gccccgtgcc cttcgccggg gctgagtggc gcgagctgtt ccggttcgtc
      241 gtctccgaag cccaccggct cgggctcgaa gtcaacatga acaatgatgc cgggtggaat
      301 ggcagcggcg ggccgtgggt gactccggac aaggccatgc agaaggtggt gtggaccgag
      361 accgacgtcg agggcccgca gcgcttcgag ggcgttctcg cgcagccaga gacggtggcc
      421 gggtactacc aggacatcac cgtgctcgcg ttcccggcgg tgggcgacta ccgtatcgct
      481 gacatcaacg gcaagtcggc cctggtgcgg caggatcgcg accagccggc cgagttcccg
      541 gacgtgcccg ccgggcagac catcaaccac gagcgcatcg tgaagctggc cgacaagatg
      601 gccgccgatg gcaagctcac gtgggatgtg ccggcaggca ggtggaccat cctccggctg
      661 ggccatacgt gcacgggagc catgaacgcg ccggcccccg aatcgggccg tgggctcgaa
      721 tgcgacaagc tcagcaagga gggcgccgag gccaacttca acgggttcat ggcccacctt
      781 atccgcgacg tcggggagag ggccggcgac acgctggtcg cgacgcatgt cgacagctgg
      841 gagaacgggt cgcagaactg gaccccgcgc ttccgcgagg cgttcaagga gttgcggggg
      901 tatgacttga tcgactacct gcccgccatc accggccggg tggtggacag cctggaggtt
      961 tcggagcgtt tcctctggga ctggcggcag acggtctccg acctgctggt cgagaactat
     1021 gcggggcaca tcgccgaact ggcccacaga gccgggatgc ggttcaccat cgaggcctac
     1081 ggcgacacca cggtcgacaa cctggcctac gccggccggg ccgatgagcc catgggcgag
     1141 ttctggtcgt ggcccagctt cggcgccgtc ggcacactga ccgagatggc ttcggcggcc
     1201 catgtctatg gaaagccgat cgtgggcgcc gaagcgttca ccgcgggcga tggagagaag
     1261 tggctctatc atccgggctg gatcaagtcc atgggcgacc gggccttctg catgggcatc
     1321 aaccggttcg tgttccaccg ctatgccctg cagccgtgga aggaccggcg gcccggcatg
     1381 tcgatgggcc cctggggtct gcattatgaa cggacacaga cgtggtggga gcagtccggc
     1441 ccgtaccacg agtacctgac tcgttgccag tacctgttgc agcagggcct gcccgtggtc
     1501 gatgtgctgt acctggcgcc ggaaggcgct ccgagggact acaacgcgcc gcccagcacc
     1561 caccgcacgg gctacaaggg cgatgcgtgc cccgctgagg tcgtcctcga gcggcttagg
     1621 gtagaggacg gtcgtctcgt ccttcccgac gggatgagct acgccgcgct gtcactgccg
     1681 ggagcggcca ccatgacgcc gaagctactc gaccggttgg cgaagcttgc ccgagacggc
     1741 gcgaccatcg tcggcacgcc gccgcggaag gctcccgggc tcacggacta tccccggtgc
     1801 gacgcggagg tccggcgagg agccagggcg ctctgggagt cgggcaaggt cgtcacgggc
     1861 aagacggcgg aggaggtcct cgccgcccgg ggtgtggccc cggacttctc ttcggaccgc
     1921 gcgctggact ttattcacaa gcgcatcggc gaggccgacg tgtacttcgt ctgtaaccca
     1981 tcaaagcata cggtcaac
//
LOCUS       DAOSPL010000959         1998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_56619_length_1998_cov_3.063304, whole genome shotgun sequence.
ACCESSION   DAOSPL010000959 DAOSPL010000000
VERSION     DAOSPL010000959.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56619_length_1998_cov_3.063304"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="PLQ54_15675"
     CDS             <1..944
                     /locus_tag="PLQ54_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOS94759.1"
                     /translation="NASRTMLYNIHQRRWDEELLRLLGIPPAMLPAVRPTSGVFEMTT
                     ADFLGTPVPVAGAVGDQQSALFGQCCFDAGSAKNTYGTGCFALMNTGDRPVASAHGLL
                     TTVGWEINGETTYALEGSVFVAGAAVQWLRDELGLIESAAESESLARSVPDANGAYLV
                     PAFVGLGAPHWDMNARGVLVGLTRGTTKAHVVRACLESIAYQSADMLAVMAEDRGAPL
                     AELRVDGGASANDLLLQFQADILGVPVLRPVDVETTARGAAFLAGLAVNYWSGPDELA
                     ACNDTGRRFEPSMDAGQRARLLAGWHRAVDRARDWER"
     gene            998..>1998
                     /locus_tag="PLQ54_15680"
     CDS             998..>1998
                     /locus_tag="PLQ54_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94760.1"
                     /translation="MVTIRGGDGCRDDTLRIQRALDSRCARVYLAPPERHYLISRPLR
                     IYSNQTLEADRSTVVRLAPGSDCLMLRNADSDRGNEHIAIVGGIWDMDNANQSLAEYQ
                     RTRDWHGDYDPERYLGVLLRMDRVKGLCLRDLTLKDPVTFGTQLGNLDQFTIEGITFD
                     YNLARTNMDGIHIHGNSRCGRIANIQGTTNDDLVALNADDGGIYEMSRGPIEDILVDG
                     LFSQNGYTAVRLLSAGSPLRRVRIANVFGTYRYNAVSLTNHQVHPGSESTFDDISVAG
                     LTCSKSTRRMDVDTSVPGWPGHSLVWVDAPAKVSCLAVSDFHRTEDAWPAETISIMPG
                     "
BASE COUNT          343 a          732 c          625 g          298 t
ORIGIN      
        1 ccaacgcctc ccgcaccatg ctctacaaca tccaccagcg gaggtgggat gaggagctcc
       61 tccgcctgct cggcatccct ccggccatgc tgcccgcggt gcggcccacc agcggcgtgt
      121 tcgagatgac cacggccgac ttcctgggca cgcccgtgcc cgtcgccggc gccgtgggcg
      181 atcagcaatc ggcgctcttt ggtcagtgct gcttcgacgc cggctcggcc aaaaacacct
      241 acggcacggg ctgcttcgcg ctgatgaaca ccggcgaccg gcctgtcgcc tcggcccacg
      301 gcctgctgac cacggtcggc tgggagatca atggcgagac aacctacgcc ctcgagggta
      361 gcgtgttcgt ggccggagcg gccgtccagt ggctgcggga tgagctcggg ctcatcgaga
      421 gcgcagccga gagcgaaagc ctcgcgcgat ccgtccccga tgccaatggc gcgtacctcg
      481 tccccgcctt cgtgggcttg ggtgcgcccc actgggatat gaacgcccga ggcgtcctcg
      541 ttggcctcac gcggggcacg accaaggccc atgtcgtccg cgcatgcctc gagtccatcg
      601 cctaccagtc ggccgacatg ctggccgtga tggccgaaga ccgaggggct ccgctggccg
      661 aactccgcgt cgatggcggc gccagcgcca acgacctgct gctgcagttc caggccgaca
      721 tcctcggcgt gcccgtgctg cgacccgtcg atgtcgagac cacggcgcgc ggcgctgcct
      781 tcctggccgg actggccgtg aactactgga gcggcccgga tgagctcgcc gcctgcaacg
      841 acacgggccg gcgcttcgag cctagcatgg acgcaggcca gcgcgcccgc ctgctggctg
      901 gatggcaccg cgcggtggac cgcgccaggg actgggaacg gtgagagagc cgccgaagag
      961 caaggtgggc gaccaagctc cagcgaagcc ttctgccatg gtcacgattc gaggcggcga
     1021 cgggtgccgc gacgacacct tacggataca gcgtgccctc gactcccgct gcgcccgcgt
     1081 ctacttggcc ccacccgagc ggcactacct gatcagccgc ccgctccgaa tctactcgaa
     1141 ccagacactt gaggcagacc ggtccacagt cgtgcgcctc gcacccggca gcgattgcct
     1201 catgctccgc aacgccgact ctgaccgagg aaacgagcac atcgccatcg tcggcggcat
     1261 ctgggacatg gacaacgcca accagtcgct cgccgagtac cagcgcacgc gcgactggca
     1321 cggcgactac gaccccgagc gttacctcgg cgtcctcctc aggatggacc gcgtcaaggg
     1381 cctgtgcctg cgcgacctca ccctcaagga tccggtgaca ttcggcaccc agctcgggaa
     1441 cctcgaccag ttcaccatcg agggcatcac ctttgactac aacctggcgc gcaccaacat
     1501 ggacggcatc cacatccacg gcaactcgcg gtgcggccgc atcgcgaaca tccagggcac
     1561 caccaacgac gacttggtcg cgctgaacgc cgacgatggc ggcatctacg agatgagccg
     1621 agggcccatc gaggacatcc tggtcgacgg gctcttctcg cagaacggct acacggccgt
     1681 acggctgctc tcggccggtt cgccgctgag gcgcgtccgc atcgccaacg tgttcggcac
     1741 ctaccgctac aatgcggtct cgctcacgaa ccaccaggtg cacccgggca gcgagagcac
     1801 cttcgacgac atctcggtcg caggcttgac atgctccaag tcgacacgca ggatggacgt
     1861 cgacaccagc gtgcccggct ggccgggcca ctccctcgtg tgggtcgacg ccccagcgaa
     1921 ggtctcgtgc ctcgccgtct cggacttcca ccgaaccgag gacgcctggc cggcggagac
     1981 tatctccatc atgccggg
//
LOCUS       DAOSPL010000960         1994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_56765_length_1994_cov_3.993811, whole genome shotgun sequence.
ACCESSION   DAOSPL010000960 DAOSPL010000000
VERSION     DAOSPL010000960.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56765_length_1994_cov_3.993811"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..992)
                     /locus_tag="PLQ54_15685"
     CDS             complement(108..992)
                     /locus_tag="PLQ54_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94761.1"
                     /translation="MSARTARILRLLLWTSAVATAALLAFVLFSGPPNGPRQVEARLR
                     RQAGEVSAALASPDPETRMGCLWALCCSDLVTGDSIYAHLGPADRARARQVVLEALEA
                     NSDGQPWLAARAISDNIRTEMHSQADKVGACLGGDVDPRARLAIASALASTGNADAMR
                     RHLSRALLGEPERRNRLWYARLLWRHDLGPVEARLLTRRAGGKPPATESAALAELKKE
                     LATGSQDAADALCFSGHLDLLSAEQKAGLAGRPGPDGRARVLARQCKGARAQRGAPVA
                     CAREGQGPSPECSAGDRS"
     gene            complement(989..1366)
                     /locus_tag="PLQ54_15690"
     CDS             complement(989..1366)
                     /locus_tag="PLQ54_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94762.1"
                     /translation="MDVLFVYTWANVLPSGAWTGDWWSVEVDINPKAGGVHAFIRQHA
                     PRSVTEDEVNLSRDQAVEVARSALRRDGGGTQAAEMLETRLVLSSPNSPTRGPVWHVT
                     LSEGESTRLDFQVDGVTGEVLRP"
     gene            complement(1388..1828)
                     /locus_tag="PLQ54_15695"
     CDS             complement(1388..1828)
                     /locus_tag="PLQ54_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94763.1"
                     /translation="MGSRAQSRGGLTRRRLHVLVLLLPAAGVVAGIGAGRGRGAGDEP
                     EEPTALATRLLAAIYTQPMEALQVEALGGRAQREGECRVVARWVDDQGARSRASLTID
                     VNREEIRTYVGPLLTDLPDPSSDGRLRTAEEFARAHRGPFTDAT"
BASE COUNT          294 a          747 c          642 g          311 t
ORIGIN      
        1 gggcaaacta cagggcatcg tctccgtcag cgacatcgtc agcaaggcca tgtaggtcgc
       61 ccggcgctgc ggtccgtcgc tctgcatgac cccagccaca cgaagggtca ggacctgtct
      121 ccggcggagc actccggaga cgggccctga ccctctcggg cacacgcaac tggtgcgccc
      181 cgctgagccc tggccccctt gcactggcgc gccagaacac gcgcgcgccc atccggacca
      241 ggtcgcccgg ccaggcccgc cttctgctcc gcgctgagca ggtcgagatg gcccgagaag
      301 cacagggcgt cggcggcatc ctggctcccg gtcgccagtt ccttcttcag ctccgcaagg
      361 gccgctgact cggtcgccgg cggcttccca ccggcgcggc gcgtcagcag tcgcgcctcc
      421 accggcccga ggtcgtgcct ccacagaagc cgggcgtacc agagccggtt gcggcgctcg
      481 ggctcgccga ggagtgctct gctgaggtgc cgccgcatcg cgtccgcgtt gccggtcgag
      541 gcgagggcgg aggcgatcgc gaggcgtgcc cgtgggtcca catcaccccc caggcacgcc
      601 cccactttgt cggcctgact gtgcatctcg gtcctgatgt tgtcagagat cgccctggca
      661 gccagccagg gttgtccgtc tgagttcgcc tccagggcct cgagaaccac ctgcctagcc
      721 ctcgctctgt ccgcgggccc gagatgggcg tagatcgagt cgccggtcac gagatcggag
      781 caacacaggg cccacaggca ccccatcctc gtctcgggat ccggcgatgc caaggccgcc
      841 gacacctccc ccgcttgtcg gcgcaagcgg gcctcgacct ggcgcggacc attgggcggg
      901 ccgctgaaca gcacgaaggc cagcagtgcg gcagtggcga ccgcgctggt ccagagcagt
      961 agccggagga tgcgcgccgt tcgcgcactc acggccgcag gacctccccc gtgaccccgt
     1021 ctacctggaa gtcgagacgg gtcgattcgc cctcagagag cgtcacgtgc cacaccgggc
     1081 cccgggtcgg cgagttgggc gaactcagaa ccagtcgagt ctccagcatc tcagccgcct
     1141 gtgtgcctcc cccatctcgg cgcagcgcac tcctggcgac ctcgaccgcc tggtcgcgcg
     1201 acaggttcac ctcgtcctcc gtgacgcttc ggggcgcatg ctggcggatg aacgcatgca
     1261 cccctccggc tttggggttg atgtcgacct ccacggacca ccagtctccc gtccatgctc
     1321 cactgggcag cacgttcgcc catgtgtaca cgaagagcac gtccatagac tgcttctcgg
     1381 agacgagcta ggtggcgtcg gtgaacggcc cgcggtgcgc ccgggcaaac tcctcggcag
     1441 tcctcaggcg cccgtctgac gatgggtcgg gcaggtctgt cagcaggggg cccacgtagg
     1501 tgcggatctc ctcgcgattg acgtcaatcg tgagcgacgc cctggatctg gccccctggt
     1561 catccaccca ccgggctacc acgcggcact ccccctctcg ctgcgcgcgg ccgcccagtg
     1621 cctcaacctg cagggcctcc atcggctgtg tgtagatggc ggccagaagc ctcgtggcga
     1681 gcgcggtggg ttcctccggc tcgtcaccgg caccccggcc gcgtccagcc ccgatccccg
     1741 caacgactcc cgcagccggc agcagcaaga cgagcacgtg gagccgacga cgggtgagtc
     1801 ccccacgaga ctgcgcgcgc gagcccatac gccacacctc cgttcgcggc ggttcgtcgt
     1861 ccccaagcgg ctcaggctag tatgctctgg ggccggccgc cgtgctgcgg ccggcccctc
     1921 gaaccctaag gcttgattga cttcggctct ttggacgcca ggtccgggac ctccaggatc
     1981 tcgccggtca gtcc
//
LOCUS       DAOSPL010000961         1994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_56785_length_1994_cov_2.587416, whole genome shotgun sequence.
ACCESSION   DAOSPL010000961 DAOSPL010000000
VERSION     DAOSPL010000961.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56785_length_1994_cov_2.587416"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PLQ54_15700"
     CDS             complement(<1..831)
                     /locus_tag="PLQ54_15700"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012864594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOS94764.1"
                     /translation="MVVLVVNCGSSSLKYILYDMRTETVAATGLIERIGGGGDQHAVV
                     HHRPTDKDRVDYESSIEDHYAAVQETVKLLTTGEGKVLNHLDEIDAVGHRVVHGGEAF
                     CSAVLINEPVIKAIEACVSLAPLHNPPNLLGIRACMAALPGKPQVAVFDTAFHQTMPR
                     RAYLYALPYELYERHGIRRYGFHGTSHYYVARRAAEMLTQMGRDGAHARIVTCHLGNG
                     CSMSAVADGVCVDTSMGMTPLEGLVMGTRSGDIDPAIIPFLEEKLGKTGAEIERMLNR
                     E"
     gene            1275..>1994
                     /locus_tag="PLQ54_15705"
     CDS             1275..>1994
                     /locus_tag="PLQ54_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94765.1"
                     /translation="MPSGALLIRPSDPVGLSSAASAACACCAVALLWAASSLAGDDSP
                     LYAPGNLLSNPSVELGIARFGEPTDWQPAGERGITLTRLPYGIGGLVLAQELPVASGP
                     AVPTVALWPPSVAYSPEGTLLVVDRGWRVLFWLTREGTVAARRGWPGVPPPPPPRMDR
                     AFRLMEEVRLILADGWPLLPPESDPGGFVPPPDPTAGRALLTGVPLAGGQVAMLWGPP
                     RPAVRLYRLDGNTELEEVRSAR"
BASE COUNT          301 a          647 c          693 g          353 t
ORIGIN      
        1 ctcgcggttg agcatccgct cgatctcggc gccggtcttg cccagcttct cctcgaggaa
       61 ggggatgatg gccgggtcga tgtcgccgct ccgggtgccc atgactagcc cctcgagcgg
      121 cgtcatgccc atcgaggtgt cgacgcacac gccatcggcg accgcgctca tgctgcagcc
      181 gttcccgagg tgacaggtca cgatgcgggc gtgggcgccg tcgcgcccca tctgggtgag
      241 catctcggcg gcgcggcggg cgacgtagta gtgactcgtc ccgtggaagc cgtagcgacg
      301 gatgccgtgg cgttcgtaga gctcataggg cagcgcatag agataggccc ggcggggcat
      361 ggtctggtga aaggcggtgt cgaacacggc gacctgcggc ttgccgggga gggcagccat
      421 gcaggcgcgg atgcccagca ggttcggggg attgtgcaac ggggccaggc tgacacaggc
      481 ctcgatggcc ttgatgaccg gctcattgat caggaccgcg ctgcagaatg cctctccgcc
      541 atggacaacc cgatggccca cggcatcgat ctcatccaga tggttgagga ccttgccctc
      601 gcccgtcgtc agcagcttga ccgtctcctg gacggcggcg tagtgatcct cgatgctgct
      661 ctcgtagtca acgcggtctt tgtcggtggg tcgatggtgg acaacagcgt gctggtcgcc
      721 tccgccgccg atgcgctcga tgaggcccgt ggcggcgacg gtctcggtgc gcatgtcata
      781 gaggatgtac ttgaggctgg agctgccgca gttgacgacg agaacgacca tgtaccttct
      841 gcaccaccca ggttgcgttt ggcccgtgcc accggtcgac gacgaccagg cacgcgaagg
      901 gccctatcgg tcgggctgac acgcagccca ctgccaaggg ccaaccatcg cgatcacgta
      961 cgaggagtgc ggggtgccac tggcgcaccc cgtgctggcg agctactgga ggaagcatac
     1021 cataccgcgc cgccgggtcg caccggagtc cgtgcgcaaa tgcacgaagt ctgcgggcgc
     1081 agaagggcag ggggcttcag agacagcccg cagcggtctg attcgcggag agaagccggg
     1141 actcggatga cgcccatgga ggcgccacca agcagacgct caacagcccg cccctgcggc
     1201 ccaggaatgt atccgcttac atttgaaaag cctattgcgc cgctaggtag tcatgactac
     1261 gattcccata cgtcgtgccc agcggggcgc ttctcatacg tccgagcgac ccagtcggtc
     1321 tgtcatctgc cgcatctgcg gcgtgcgcct gctgcgccgt cgcactgctc tgggctgcct
     1381 cctctcttgc cggcgatgac tccccgctgt atgcgcccgg caacctgctc tcgaacccct
     1441 cggtggagct cggcattgcg cgtttcggcg agccaactga ctggcagccg gcgggtgaac
     1501 gaggcatcac gctgactcgt ctgccctacg gcattggggg cctagtgctc gcccaggagc
     1561 tgccggtcgc ttcgggcccg gcggtcccca cggtggccct ctggccgccg tcggttgcgt
     1621 actcgcccga aggcacgttg ctggttgtcg accgcggctg gcgtgtgctg ttctggctca
     1681 cccgcgaggg cactgtggcc gcgcgccggg gttggcctgg cgtgccccct cctcccccgc
     1741 ctcgcatgga tcgggccttc aggctgatgg aggaggtcag gttgatcctg gcagacgggt
     1801 ggccgctgtt gccgccggag tcggatccgg ggggcttcgt cccgccgccc gatcccacag
     1861 cagggagggc tctgctgact ggcgttccgc tggctggcgg ccaagtggcg atgctctggg
     1921 gaccaccgcg gccggcagtg aggctctacc gtctggatgg caacacggag cttgaggagg
     1981 tcagatcggc gcgg
//
LOCUS       DAOSPL010000962         1992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_56855_length_1992_cov_2.394424, whole genome shotgun sequence.
ACCESSION   DAOSPL010000962 DAOSPL010000000
VERSION     DAOSPL010000962.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56855_length_1992_cov_2.394424"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PLQ54_15710"
     CDS             complement(<1..481)
                     /locus_tag="PLQ54_15710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS94766.1"
                     /translation="MRRMRPRSGQAPRSRRGFTLVEMMVAVGVLATLMGAIWGVFMMG
                     MRLYSIHRAAYTSDSNASMALERGLRDLADAAIITYASSDYFTYRKPAKGPDGFYELE
                     RHGGIAIQMTAVTLGDEMKLYKLGEAVYLDNTSDAEPPIELASGVTNVHFLYDGLEAS
                     "
     gene            complement(512..964)
                     /locus_tag="PLQ54_15715"
     CDS             complement(512..964)
                     /locus_tag="PLQ54_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS94767.1"
                     /translation="MRSAGTAGRPRGRRGFTLIEILLSVVILGVAMVALMSMLAHAGR
                     TQRFARYEMLCSTAAQDLLARLEGGSFSDITPERAPTYAAEMEAALLEQGVPDPTAEV
                     EIAPWPDTSTAHLRRVTVTVQFGGDTVRVKDLGSIRLETLFCDSVKEL"
     gene            complement(1295..>1992)
                     /locus_tag="PLQ54_15720"
     CDS             complement(1295..>1992)
                     /locus_tag="PLQ54_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94768.1"
                     /translation="AAAATLATFAADAVVLAEAAEVVAAGVRSGGEGEPGGNEDPANQ
                     GGNTEDGEGETERNRLRDGSQCSVGEGGRIRVERPDGTVIEGYVDGDQQIRRGDGWLA
                     DGEQEQGEGETQRIRLRLQNGGQCLVDPPGTPGGAFECQGPGTWVIEVTQNEDGSLVV
                     AFASVEITVVRNEDGTLTATLGDGSNWQVTLNEDGTISATSPSGSTWVVSMTQGAIMV
                     TTPSGASHELTVE"
BASE COUNT          301 a          746 c          613 g          332 t
ORIGIN      
        1 ccgaggcctc cagcccgtcg tacaggaagt ggacgttggt gacaccgctg gccagctcga
       61 tcggcggctc cgcatcgctc gtattgtcca ggtacacggc ctcgccgagc ttgtagagct
      121 tcatctcgtc acccagggtc accgctgtca tctggatggc aatgcccccg tggcgctcca
      181 gttcgtagaa cccgtcaggg cccttcgcgg gcttgcggta cgtgaagtag tccgagctgg
      241 cgtacgtgat gatcgcggca tcggccaggt ctcgcaggcc acgctccagc gccatggacg
      301 cattggagtc gctggtgtag gccgcccgat gaatcgagta caaccgcatg cccatcatga
      361 agacgcccca tatggcgccc atcagcgttg cgagtacccc caccgccacc atcatctcga
      421 ccagggtgaa gccccggcgc gagcgggggg cttgccccga tcggggccgc atccttctca
      481 tcgcctatcg cctcctcaca ggcgccccgg gctatagctc cttgacgctg tcgcagaaca
      541 gtgtctccag tcgaatggac cctaagtcct taacccgcac agtgtcgccg ccgaactgga
      601 cggtcaccgt gacccgccgt aggtgcgcgg tcgatgtgtc cggccagggc gcgatctcga
      661 cctcggccgt cgggtcgggc acgccctgct cgagcagggc cgcctccatc tcggccgcgt
      721 acgtcggcgc cctttcgggg gtgatatccg agaacgaacc gccttccagt cgtgcgagaa
      781 gatcctgggc cgccgtcgag cacagcatct catagcgcgc gaagcgctgg gtgcgcccgg
      841 catgagccag catggacatc agggccacca tcgccacacc cagaatgacg accgagagca
      901 ggatctcgat cagcgtgaag ccgcggcggc ctcgaggccg cccggcggtg cctgcagatc
      961 gcatcacaaa cgcctccctc cggccccgag cttcatgccc gggaagcggg gcgtggacgc
     1021 gtcgcgacag gcccgcgatg ctcggcgggg ccaggcgcgg catgtcctcc agcgccatta
     1081 ttccgcaaac gggcccgacc cgagggcgaa tacccgttgg cgtctgcacc acagctctgc
     1141 gcgggtacgc gggaaccgcg ccccacacac ggctcaccgc gcggcgggac gccgctatct
     1201 ggcgcgtgcc gccgcggccg cctcaaccgt cgcacagacg aggagagccc gccgggccaa
     1261 caccccgacg ggctccctcg cggcgctact gggcctactc gaccgtcagc tcatggctgg
     1321 cccccgatgg tgtcgtgacc atgatcgctc cctgggtcat gctgactacc caggtgctcc
     1381 cgctcgggct tgtcgcgctg atggtgccat cctcgttcaa cgtgacctgc cagttcgagc
     1441 cgtcgccgag cgtcgcggtc agggtgccgt cctcgtttcg gaccaccgtg atctctaccg
     1501 acgcgaaagc cacaaccagt gagccatctt cgttctgcgt aacctcaatg acccatgtgc
     1561 cggggccttg gcactcgaac gcgccgccgg gagtccccgg cggatccacg aggcactggc
     1621 cgccgttctg caggcgcagc cggatccgct gtgtctcgcc ctcgccctgt tcctgctctc
     1681 catcggcgag ccacccgtcg ccgcggcgga tctgctgatc tccgtccacg tagccttcga
     1741 tcaccgttcc gtcggggcgc tcgacccgta tccggccgcc ctcgccgacg gagcactgac
     1801 tcccgtcgcg aagccgattc ctctcggtct caccctcgcc atcctccgtg ttgccgccct
     1861 ggtttgccgg gtcctcattg ccaccaggct ctccctcgcc tccggaccga accccggcgg
     1921 cgaccacctc ggcggcctcc gccagcacca ccgcgtccgc ggcgaatgtg gcgagagtcg
     1981 cggcggctgc gg
//
LOCUS       DAOSPL010000963         1991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_56901_length_1991_cov_2.173554, whole genome shotgun sequence.
ACCESSION   DAOSPL010000963 DAOSPL010000000
VERSION     DAOSPL010000963.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56901_length_1991_cov_2.173554"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PLQ54_15725"
     CDS             <1..945
                     /locus_tag="PLQ54_15725"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94769.1"
                     /translation="PEHVVPAEPSIPGFEAFHSTDCPTVYQIPGKTGWEMSFIAFNGR
                     GYNSFVAHSDDLVHWTTPRLAMGFGPEGDFDHGGCVVGAYLYESYDLDAPRMLKRRHG
                     RFWTLYGCYPLQGGYELRPGYEGVAVSDDGLSWRRAKDSYILSIHDPDAAEWERDCVY
                     QPWLVEHEGRFYNFYNAANGPIEQSGLALSEDLLAWRRYPGNPIVRNRAGGYDQNFAS
                     DPKVYRHGDHWVMFYFGVAPDGHAHIMLAFSRDLVNWTARAEPIYKAGGHPTGLDEQY
                     AHKTSLVYNPANDTYYLYYCAVGSLGRGIGLLTSKPLG"
     gene            1119..1577
                     /locus_tag="PLQ54_15730"
     CDS             1119..1577
                     /locus_tag="PLQ54_15730"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS94770.1"
                     /translation="MRDLRRRRPDASEQWIEHLRSALCDLRDITATVRGDCRHCIMRV
                     EYASDLTVPAWLVSDGTLRLLALTLLAYYPWDEAGAFMIEEPENGVHPGAIETVLDSL
                     RSMWDHQVLLATHSPALLWMATTGQVPCFARDVNGNTDIVAGEDHPRLTA"
BASE COUNT          344 a          665 c          614 g          368 t
ORIGIN      
        1 cccgagcacg tggtgcctgc cgagcccagc atccccggtt tcgaggcctt ccactcaacc
       61 gactgcccta cggtctacca gatcccgggc aagaccgggt gggagatgag tttcatcgct
      121 ttcaacggcc ggggctataa ctcgttcgtg gcccatagcg atgacctggt ccattggaca
      181 accccacgcc tcgcaatggg ctttgggcct gagggcgact tcgaccatgg cggttgtgtc
      241 gtgggtgcct atctctacga gtcctacgac ctcgatgcgc cccggatgct gaagaggcgc
      301 cacgggcggt tctggacctt gtatggctgc tacccgctcc agggcggcta cgaacttcga
      361 ccgggctatg agggcgttgc ggttagcgat gacggccttt cgtggcgccg agcgaaggac
      421 tcatacatcc tctccatcca cgaccccgat gctgccgagt gggaacgcga ctgcgtctac
      481 cagccttggc tcgtcgaaca cgaggggcgc ttctacaact tctacaacgc cgccaacgga
      541 cccatcgagc agagcggcct ggcgctctcg gaggacctgc tggcctggcg ccgctacccc
      601 ggcaacccga tcgtccgcaa tcgcgccggc gggtacgacc agaacttcgc ctccgacccc
      661 aaggtctatc ggcacggcga ccactgggtc atgttctact tcggcgtggc acccgacgga
      721 cacgcccaca ttatgctggc cttctcgcgc gatctggtga actggacggc ccgcgccgag
      781 cccatctaca aggccggcgg ccaccccacc ggcctcgatg agcagtacgc ccacaagacc
      841 tcgctcgtct acaacccagc gaatgatacc tactacctct actactgtgc tgtcgggagt
      901 ctcggccgcg gcatcggact cctcaccagc aagcccctgg gatagggtgt gtcggaaggc
      961 gacgacgcgc gccaggggct gtacccggag atacactggg tacgcggtct cctctctcgg
     1021 ggcatccact atatccacct ggacgccgct cagatgagac ggccctgccc gcccaggacg
     1081 gccccctggc caatgacggg gcgaacctgg cttgggctat ccgcgacctt cggaggaggc
     1141 gccctgacgc ctctgagcag tggatcgagc acttgcgctc tgccctctgt gacctccgcg
     1201 acattactgc gacggtgagg ggtgactgcc ggcattgcat catgcgggtc gagtatgcct
     1261 cggacctcac cgtgcctgcg tggttggtct ccgacggcac cctgcgtctc ctcgctctga
     1321 cactgctcgc gtactatccg tgggacgagg ccggcgcgtt catgatcgaa gagcccgaga
     1381 acggcgttca cccaggagcc attgagacgg tcctcgacag tctgaggtcc atgtgggacc
     1441 accaggtgct tctggcgacc cactcgcctg cattgctctg gatggcgaca acggggcagg
     1501 tgccctgttt cgcgcgcgac gtgaatggta acacggacat agttgccggc gaagaccatc
     1561 cgcggctgac tgcgtagcag gacgaggtcg acctcagcct cctgaatgcg gccggtgtac
     1621 tcgcgtgacg aaggtttctg agaactacca gactgacctc gtagtgcttg ctgcagactc
     1681 caagatgcgg gccacgcttg acacgttgct ctctctccgg cgcaaggcgc tcgggttgga
     1741 cccgttcagc ttcgatatca tagtgcaccc ccagagggat gccggcgtag ttgcgagctg
     1801 tgtccagatc ctccgggcat gctcacgacc catcgactgg cgctcgtggt gttcgactat
     1861 cacggatccg gccgaccggg ggacccagac tccgtctgca ggcacgtcga aaagcagctc
     1921 aacgggggcg gctggggcga tcgcgcgcgc tatgcatcag cccggaactc gaggcgtggg
     1981 tgccgttcgg c
//
LOCUS       DAOSPL010000964         1990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_56921_length_1990_cov_3.457364, whole genome shotgun sequence.
ACCESSION   DAOSPL010000964 DAOSPL010000000
VERSION     DAOSPL010000964.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56921_length_1990_cov_3.457364"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1990
                     /locus_tag="PLQ54_15735"
     CDS             <1..>1990
                     /locus_tag="PLQ54_15735"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOS94771.1"
                     /translation="EAYKKASAPIPETMLAWQLFGTGLENLGRFARPCRVPTPQFGPR
                     ELLARVDAAGLCFSDIKILNLGSEHPRIQGRNLETDPVIMGHEVAITLVGVGEQLAGQ
                     YRIGQRFVVQADIYYKGVGLAFGYQLPGALCQYVRLGEEILNGDDGCYLIPVADSTGY
                     AEAALSEPWACVECAYRMRHRVGLHDGGVTLVIANGGTLEYELGAEILAGGKPSKIVA
                     CGVPAGLAGQLKEAARCFGCDVVEVAAADVDTLSSAHTGGQGFGDIIVLGSESADLVE
                     SASRCLGKGGVLLLAADRPLDRRIQVDVGRIHYEATQYIGTTEANLSTAYVRTRGSEL
                     AAGGAAWIVGAAGPMGQMHVQRALELRTPPRRVVATDVDPARLGTVEERYGDLARERG
                     IELICLNPVAMGQEAFETRLRELEPEGFDDVIVMAPVAALVESSAPWLKPGGVMNVFA
                     GLPRGTMAALDLSAVYLRDNRVIGTSGSAISDLEFTLGKTESGELSPNRSVAAVGGIE
                     AAWEGLRGVKTGRFAGKVVIFPQLERVPLIPLQAMEACMPAVAAALTPKGEWTRDAEK
                     AFIASGLRPSRTGCQRLGGRVALVTGGAQGLGRALAERLASLAVRTVITDINAEGVRK
                     AAAEIADSTGVPVIGEVADVTSAEDMARNELYVQVSHEQ"
BASE COUNT          311 a          601 c          732 g          346 t
ORIGIN      
        1 cgaggcctac aagaaggcgt ccgctcccat accggagacc atgctggcct ggcagctttt
       61 cggtacgggc ctcgagaacc tgggacggtt cgctcggcca tgccgcgtac ccacgccgca
      121 gtttggtccc cgtgagctcc tggcacgggt ggatgccgcg ggcctgtgct tctcggacat
      181 caagatcctg aacctcggat ccgagcatcc gcggatccag ggacggaacc tcgagacaga
      241 tccggtcatc atgggccacg aggtcgccat caccctggtg ggggtcggcg agcagctggc
      301 cggtcagtat cggattggtc agcgcttcgt ggtgcaggcg gacatctact acaagggtgt
      361 gggcctggcc ttcgggtacc agctcccggg agcgctctgt cagtatgtgc gcttgggcga
      421 ggagatcctg aacggcgatg acggctgcta tctcatcccc gtggccgact cgacgggcta
      481 cgccgaagct gcgctgtcgg aaccgtgggc ctgtgtcgaa tgcgcgtacc ggatgcggca
      541 ccgcgtgggt ctgcacgacg gtggagtcac cctcgtgatc gccaatggtg ggacgctgga
      601 gtacgagctg ggggccgaga ttctcgccgg cggcaagcca tcgaagatcg tggcttgcgg
      661 ggtaccggcg ggccttgcgg gccagctcaa agaggccgcc cggtgctttg ggtgtgacgt
      721 cgtggaggtg gcggcggccg atgtcgacac gctatcgtcg gcgcatacgg gcggacaggg
      781 cttcggcgat atcatcgtgc tgggtagtga gagcgcggat ctcgtcgaat ctgcctcgcg
      841 ctgcctcggc aaggggggcg tgcttctcct ggcggcggac cggccgctcg atcgcaggat
      901 ccaggtcgat gtgggtcgca tccactatga ggcgactcag tacatcggga cgaccgaagc
      961 gaacctgagc acagcctacg tcagaacgcg gggctccgag ctggcagccg gcggtgcggc
     1021 ctggatcgtt ggtgcggccg gaccgatggg gcagatgcac gttcagcgcg cgctcgaact
     1081 gcgtacgccg ccccggcgcg tggttgccac cgacgtggat ccggctcggc tcgggaccgt
     1141 cgaggagcgc tatggagatc tggcccgcga gcgcggcatc gagctgatct gccttaaccc
     1201 ggtcgccatg ggccaggagg ctttcgagac tcgcctacgc gagcttgagc ctgaaggttt
     1261 cgacgacgtc atcgtgatgg ctcccgtggc cgccctggtt gagtcctcgg caccgtggct
     1321 caagccgggt ggcgtcatga acgtgtttgc cggactccct cgaggtacca tggctgccct
     1381 ggatctgagt gcggtgtatc tgagggacaa tcgcgtgatc ggcaccagcg ggtcggccat
     1441 ttcggatctc gagttcaccc tcggcaagac cgagagcggc gagttgtctc ccaacaggtc
     1501 cgtggcggct gtgggcggca tcgaggcggc ctgggaggga ctgcgtggag tcaagaccgg
     1561 tcgctttgca ggcaaagtcg tgatcttccc ccaacttgag cgcgtgccac tgatcccgct
     1621 tcaggccatg gaggcgtgca tgccggccgt tgcggccgcg ctcacgccca agggggagtg
     1681 gactcgtgac gcggagaagg cgttcatagc gagcgggctg cggccctcac gcaccggttg
     1741 ccagcggctg ggcggccggg tggctctggt caccggcggc gcccagggcc tgggccgcgc
     1801 actggcagag cgtttggcca gtttggccgt gcggacggtc atcacggaca tcaacgccga
     1861 gggcgtgcgg aaggcggccg cggagatcgc cgacagcacg ggggtgcccg tgatcgggga
     1921 agtggcggat gtgacctcgg ccgaggacat ggcccgcaac gagttgtacg tgcaggtcag
     1981 ccacgagcag
//
LOCUS       DAOSPL010000965         1988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_57012_length_1988_cov_2.371961, whole genome shotgun sequence.
ACCESSION   DAOSPL010000965 DAOSPL010000000
VERSION     DAOSPL010000965.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57012_length_1988_cov_2.371961"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(367..1638)
                     /locus_tag="PLQ54_15740"
     CDS             complement(367..1638)
                     /locus_tag="PLQ54_15740"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOS94772.1"
                     /translation="MAQTPRPIASPAGRRYDVITLGGATQDVFVRTDLSKIITTQDVM
                     NSTSLLAFDYPSKVNVDDVWFSSGGGATNTAVSFARFGLRTACVAMVGDDTPAEAIRA
                     ELAAEGVDTHLVATAAGAKTGYSVVLNSFQGDRTVLAYRGANSRIGRENIPWDDLSGA
                     AWLYVSSLTGSSGDVLDEAARFAENHGVNLALNPGNTQIRMGMRGLAKILSLVEILFL
                     NKQEAVQLTNVQWTRRVALASLCNLCGACVDICPVSLFRIADGRLYIGDERKCIRCRE
                     CLDHCPTRAIQMEPWASNLDPILVAIKELGPKIVVITDGGNGAQAYDGETRYIVPPCK
                     AQVVDTLGAGDAFGAAFTAAIIRGHDIPTALLWASANAASVVGQTGAKPGILHLADVE
                     QFIQEHHPGPESIRTGTIRKDALSEMVDTER"
     gene            complement(1706..>1988)
                     /locus_tag="PLQ54_15745"
     CDS             complement(1706..>1988)
                     /locus_tag="PLQ54_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94773.1"
                     /translation="PHPQQLVYAFVCGFLPVFAGAVVLGAKMGHEGDFSDDAAQWVVY
                     GLSGSYVLFVLAMYRIYHLVPAAGCMPYVLRLLVGYLYRQKWGETWETG"
BASE COUNT          345 a          666 c          625 g          352 t
ORIGIN      
        1 gggatccgcg ccctccggcc gctcatcggg gacggcctca tcgatgtgct tctgcgtgat
       61 gatgcgccgg cggacgagtt cgcggatgac cagaccatcg atgtcgggcg tctgcccctt
      121 gaggctcacc tggatcctct ccccacggcg ggcccccggc acggaccaac cgaaacgaag
      181 ggccacgccc aggaactggc cgcgtggctt cctagtctga tgcccactcc ggccccaccg
      241 ttgggccgtc tgcagatatg gacgcactcc cgcctggtcg gttgcggttc gcaaccaaag
      301 gggtggacgt ccgcctgcgg gtcttgttca gaccaccagg gggtctgcga cactggggtt
      361 gcgcggttac cgttccgtat ccaccatctc ggacagggca tccttccgga tcgtgccggt
      421 ccggatcgac tcgggccccg gatggtgctc ctggatgaac tgctcgacat ccgccagatg
      481 cagaatcccg ggtttcgcac ccgtctggcc caccaccgag gccgcgttgg cgctggccca
      541 cagcagcgcc gtcgggatgt cgtgtccgcg gatgatcgct gccgtgaagg ccgcaccgaa
      601 ggcgtcgccc gctcccagag tgtcgacgac ctgtgctttg cacggcggca cgatgtaccg
      661 ggtctctcca tcgtacgcct gggccccatt tccgccgtcg gtaatcacca cgatcttcgg
      721 acctagttcc ttgatggcca ccaggatcgg gtccagattg ctggcccaag gctccatctg
      781 gatggcgcga gtcggacagt ggtccaggca ctctcggcac cggatacact tgcgttcgtc
      841 accgatgtac agccggccgt cggcgatccg gaagaggcta acggggcaga tatccacaca
      901 cgcgccacag aggttgcaca aggaagccaa ggccactcgt cgcgtccact gcacgttggt
      961 gagctgtacg gcctcctgct tgttgagaaa cagtatctca acgagcgaca ggatcttggc
     1021 cagtcctcgc atccccatgc ggatctgtgt gttgcccggg ttgagcgcca gattcactcc
     1081 atggttctcc gcaaagcggg cggcttcatc cagcacatcg cccgaagacc cggtcagcga
     1141 tgacacgtac agccacgcgg caccgctgag gtcgtcccac gggatgttct cccggccgat
     1201 ccggctgttc gctccccggt acgcaagcac cgtccgatcg ccctggaagc tgttgagtac
     1261 gacggagtag ccggtcttcg cgccggccgc cgtcgccacg agatgggtgt cgacgccttc
     1321 ggctgcgagt tcggcccgaa tcgcttcggc cggcgtgtca tcgcccacca tggccacgca
     1381 cgccgtgcgc agcccgaatc gggcgaagct caccgccgtg ttcgtggcac ctccgcctga
     1441 ggagaaccac acatcatcca cgttgacctt cgatgggtaa tcgaaggcca gcaggctggt
     1501 gctgttcatg acgtcctggg tcgtgatgat cttggataga tcggttcgca cgaagacatc
     1561 ctgggtggcg ccgcccaggg tgatcacgtc gtagcgccgt ccggcgggcg atgcaatggg
     1621 gcgaggcgtc tgggccatcg gggaaccgtc cgatcggtat agggaggtca cgcaccgtcc
     1681 attctagcag aggtgggcgg tgtagctacc cggtctccca cgtctcgccc cacttctggc
     1741 ggtacaagta gcccaccagc agtcgcagta catagggcat acacccggcc gccgggacca
     1801 ggtgatagat gcggtacatg gccagcacga agagcacata tgagcccgag agaccgtaga
     1861 ccacccactg cgcggcatca tcgctgaagt cgccctcgtg ccccatcttg gcgcccagga
     1921 cgacggcgcc cgcgaacacg ggcaggaacc cgcagacaaa cgcgtagacc aactgctgcg
     1981 ggtggggg
//
LOCUS       DAOSPL010000966         1987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_57036_length_1987_cov_2.960663, whole genome shotgun sequence.
ACCESSION   DAOSPL010000966 DAOSPL010000000
VERSION     DAOSPL010000966.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57036_length_1987_cov_2.960663"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLQ54_15750"
     CDS             <1..317
                     /locus_tag="PLQ54_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOS94774.1"
                     /translation="HYPLIELLPGVDVVVGAGGYNLFHECQAVGVPAVFVPQRRRYDE
                     QFARVAGATVASSPEHLERILASHVAAGPRLRPAPRYTNGAASAAGALLELLGASDAS
                     IC"
     gene            complement(242..1330)
                     /locus_tag="PLQ54_15755"
     CDS             complement(242..1330)
                     /locus_tag="PLQ54_15755"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS94775.1"
                     /translation="MRELRTAVAGEAPPTFIRCAKIKLTPRCNLRCLFCNYWTMNACP
                     ELGTARWREVIAELASLGCAKIHFSGGEPLLRSDVFELLECCTAHGMRANLTTNGTLI
                     NQRDVAERLLATGVRSVSISVDSPEPRLHDRIRGRRGALKATLRGIELLADVRARQRH
                     RVSLRINTVLTRRNYTRLPEILRLAGELGCQDVHPMPVDERGPRRVRLSAGEIEEYNT
                     YIAPQVQDLRTRYGFPEAQLLVWPFGTTEADRKLSKHGCYARRYYKRHLCYAPWLHTF
                     IAWDGAVYLCCMARGKTPPTGNVAGQRVAEVFTGQAYETIRGQFRVARPKVCRYCDNF
                     ILENQQIEASLAPRSSSSAPAADAAPLV"
     gene            complement(1373..>1987)
                     /locus_tag="PLQ54_15760"
     CDS             complement(1373..>1987)
                     /locus_tag="PLQ54_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94776.1"
                     /translation="ALAALIHCIVMYSREARLNFEDRKITSAAAFMAVLAQDVPRKGK
                     CQITVTDGNLRLVDRRKTGNILVPRRVDTLQGNWLDVRTDLADGNDLVVTVERTEHRK
                     FKYKKGSWRSRGTRIAEEIHVKVRLNPVAYPNHAALDPTGMMTAPTGADLTVRRARIL
                     PGPRPTLAVDLVANGNAQPELLLSAVSLAYQLLAPARPQPVQGA"
BASE COUNT          297 a          625 c          699 g          366 t
ORIGIN      
        1 tccactatcc cctcatcgag ctgctgcccg gagtcgacgt tgtggtcggc gcgggggggt
       61 acaacttgtt ccacgagtgc caggcggtgg gcgttccggc cgtgttcgtc cctcagcggc
      121 gccgttacga tgagcagttc gcccgagtgg ccggggcgac ggtggccagc agccccgagc
      181 acctcgagcg catccttgcc tcgcacgtcg cggcggggcc caggctgcgt cctgccccgc
      241 gctacaccaa tggcgcggcg tcggcagccg gggcgctgct cgagcttctg ggggccagcg
      301 atgcttcgat ctgctgattc tcgaggatga agttgtcgca gtagcggcat actttggggc
      361 gggcgacgcg gaactggccc cggatggttt cgtacgcctg tccagtgaag acttcggcca
      421 cccgctggcc agcgacatta cccgttggcg gtgtcttccc gcgggccatg cagcagaggt
      481 acacggcccc atcccatgcg atgaacgtat gtagccatgg cgcgtagcac aggtgccgct
      541 tgtagtacct ccgggcgtaa cagccgtgct tggagagctt ccggtcggcc tcggtagtcc
      601 cgaacggcca cacgagcagc tgggcctcgg ggaatccata gcgtgtccgc agatcctgca
      661 cctgtggcgc gatgtacgtg ttgtactcct cgatctcccc ggcagagagc cgcacacgtc
      721 gaggtccgcg ttcatcgacg ggcatgggat gaacgtcctg gcatcccagc tcgccggcca
      781 gtcgcagtat ctccggcagc cgcgtgtagt tgcgccgagt gaggaccgtg ttgatgcgca
      841 ggctcacccg gtgacgctgc cttgcgcgca catcggccag cagctcaatg ccacggagcg
      901 tggccttcag ggcgccccgg cggcctcgga tgcggtcgtg cagccgcggt tcggggctgt
      961 cgaccgagat tgatacgctg cggacgccgg tggcaaggag ccgctccgcg acatcgcgct
     1021 ggttgatcag cgtcccgttg gtcgtgaggt tggcccgcat gccgtgggcg gtgcagcatt
     1081 cgagcagctc gaacacgtcg ctccggagca ggggttcgcc tccgctgaag tggatcttgg
     1141 cgcagcccag gctggccagt tcggcgatga cctcgcgcca ccgggcggtg ccaagctccg
     1201 ggcaagcgtt catcgtccag tagttgcaga agagacaccg caggttgcag cgaggcgtga
     1261 gtttgatctt ggcgcatcgg atgaatgtgg ggggggcttc accggccacg gcggtgcgca
     1321 gctcgcgcat tcggccgggg tcgctagcga gctcagggat gttgtgaatg gcctacgccc
     1381 cctggaccgg ctgcggccgc gccggagcca gcagttggta ggccagcgag accgcgctca
     1441 atagcagttc cggctgagcg ttgccgttcg ctaccaggtc cacggccaat gtgggccgag
     1501 ggccgggcag gatgcgggcc cggcgaaccg tcaggtcggc gccggtgggg gctgtcatca
     1561 tgccggtggg gtcgagagcc gcatggttcg ggtaggccac cgggttcagc cgaaccttca
     1621 cgtggatctc ctcggcgatc cgtgtgccgc gggatcgcca tgagcccttc ttgtacttga
     1681 acttccggtg ctccgtgcgc tcaacggtga cgaccaggtc attcccgtcg gccagatcgg
     1741 ttctgacatc gagccagttg ccctgcaggg tgtccactcg tcggggaacg aggatgttgc
     1801 ctgtcttgcg gcgatcgacg aggcgcaggt tgccgtcggt gaccgtgatc tggcatttgc
     1861 ccttacgggg cacatcctgg gctagcacgg ccatgaaggc agccgccgag gtgatcttgc
     1921 ggtcctcgaa gttcagccgc gcctcacgcg agtacatcac gatgcaatgg atcagggcgg
     1981 cgagcgc
//
LOCUS       DAOSPL010000967         1987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_57049_length_1987_cov_2.081263, whole genome shotgun sequence.
ACCESSION   DAOSPL010000967 DAOSPL010000000
VERSION     DAOSPL010000967.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57049_length_1987_cov_2.081263"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PLQ54_15765"
     CDS             <1..480
                     /locus_tag="PLQ54_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94777.1"
                     /translation="EHTIYGEVAWDSNISWLVPSAALLDVQVLYDGDWDVHLGGSYVR
                     AAYAAADPATLLTPYTRAWGNEPFAGFFYQRLMTPLAFGKGEATAYLKATFHDDSRDW
                     RFAYIQSSIKGNSMASVGTDIPIGGDFDLNVDVAQTLFGWVNNTPRTLVRMGVSVPF"
     gene            595..1563
                     /locus_tag="PLQ54_15770"
     CDS             595..1563
                     /locus_tag="PLQ54_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004943003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94778.1"
                     /translation="MGGTTLVERDLTTKLCEMRATGEFVAALAEARARPRRARPSPDG
                     RLALIAADHPARGVTEALGVSLRMADRHDYLSRIMRALEAPLIDGVMGTSDVLEDLLV
                     ADLLRSREARGALLRDRLHIFGYNRGGLSGTTFELDDRVTGYTPDGLQRSGLDGGKVM
                     VRIDRTSRDSGATLEYAAAAVEACGRLGLVTFVEPLPVERLEGRLRVQRTVEALAWAA
                     GIASGLGSTSVRTWLKLPWCEGFAAVARATTLPIVLLGGPAEGEPERTISELGEAMRC
                     APNVRGALCGRNVLYPGDSDPLEIAQRIAEVVHGTMNGAAPAPVVE"
     gene            1595..>1987
                     /locus_tag="PLQ54_15775"
     CDS             1595..>1987
                     /locus_tag="PLQ54_15775"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOS94779.1"
                     /translation="MRIGVVGNINMDFVLRGQRLPREGETLVADGLHVFGGGKAANQA
                     VAARRLGADVSIVGCLGTDDLGDRALALLDEAGVDTAFVVRTQRCHTGTALVFVDRSG
                     RNSIVTALGANDDPEGTRGAVEGATKLLG"
BASE COUNT          314 a          605 c          727 g          341 t
ORIGIN      
        1 gagcacacga tctacggcga ggtcgcctgg gatagcaaca tctcctggct ggtcccgtcg
       61 gcagccttgt tggatgtcca ggtcctgtat gacggggact gggacgttca cttgggcgga
      121 tcgtatgtcc gcgccgcgta tgccgccgct gatcctgcga ccctgctgac cccgtacacc
      181 cgtgcgtggg gcaacgagcc gtttgctggg ttcttctatc agcgcctgat gaccccgctg
      241 gctttcggca agggcgaggc gacggcctac ctgaaggcga ccttccatga cgacagccgc
      301 gactggcggt tcgcgtacat ccagagcagc atcaagggca actccatggc ttcggtcggg
      361 accgatatcc ccatcggcgg ggacttcgat ctcaacgtcg acgtggcgca gacgctgttc
      421 ggctgggtga acaacacccc gcgtacgctg gtccgcatgg gtgtgagcgt gcccttctag
      481 ggttcgccca atccaatcgg cagacgcgac ggacggctcg gcttcggccg ggccgtccgt
      541 ttgtgtcacg ggcggcatgg cgccgggcca acccggtagc cggagacgcg gggcgtgggg
      601 ggcacgacat tggtcgagag agacctgaca accaagctct gcgagatgcg ggccacgggc
      661 gagttcgtcg ccgccctggc tgaagcacgg gcaagacccc gacgggcccg gccatccccg
      721 gacggacggc tcgctctcat cgcggccgac cacccggcgc gaggcgtgac ggaggcactg
      781 ggagtctcgc tgcgcatggc cgaccggcac gactacctct ctcggattat gcgggctctg
      841 gaggccccgc tgatcgacgg ggtgatgggg acttccgacg ttctcgagga cctgctcgtg
      901 gcggacctgc tcaggtcgcg ggaggcgcga ggggccttgc tgcgggaccg cctccatatc
      961 ttcggctaca accgaggtgg cctctccggg acgacatttg agctcgacga ccgggtgact
     1021 gggtacaccc cggacgggct ccaacgctcg ggactcgacg gcggcaaggt catggtgagg
     1081 atcgaccgga catcaaggga ctccggggcg acactcgagt atgcggcagc ggcagtcgag
     1141 gcttgcggac gcctgggact ggtcacgttc gtcgagccgc ttccggtgga gaggctggag
     1201 gggcggcttc gggtgcagag aaccgtagag gcactggcct gggcggcggg gatcgcatcg
     1261 gggctgggtt cgacctcagt ccgcacgtgg ctcaagcttc cgtggtgcga gggcttcgct
     1321 gccgttgcca gggctacaac gctgcccatt gtgctgctgg gcggtccggc agagggagag
     1381 ccggagagaa ccatctcgga gctcggcgag gcaatgcgtt gcgctcccaa cgtccgaggc
     1441 gccctgtgcg gtcggaatgt gctctacccg ggcgacagcg atccgctcga gatagcgcag
     1501 cggatcgctg aggttgtgca cggcaccatg aacggtgcgg caccggctcc cgttgtggaa
     1561 tgagtactgt ggggtgccgt ctggggtcgc tggtatgaga attggcgtag tgggtaacat
     1621 taacatggac ttcgtccttc gagggcagcg tctgcctcgg gagggcgaga cgcttgtggc
     1681 tgacgggctc catgtctttg gggggggcaa ggccgcgaac caggccgtgg cggcccgaag
     1741 actgggcgcc gacgtgtcca tcgtcggctg ccttgggacg gacgacctgg gagaccgggc
     1801 gctggccctc ttggacgagg ctggcgttga caccgcattc gtggtgcgga cccaacggtg
     1861 ccacacgggc actgccctcg tgttcgttga ccgatcgggg aggaactcca tcgttacggc
     1921 gctgggcgcc aacgatgacc ccgagggcac tcggggcgca gtggagggcg ccaccaaact
     1981 gctgggg
//
LOCUS       DAOSPL010000968         1985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_57103_length_1985_cov_4.565803, whole genome shotgun sequence.
ACCESSION   DAOSPL010000968 DAOSPL010000000
VERSION     DAOSPL010000968.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57103_length_1985_cov_4.565803"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..1307
                     /locus_tag="PLQ54_15780"
     CDS             270..1307
                     /locus_tag="PLQ54_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94780.1"
                     /translation="MRASRDVTQVQRDVALVRIWCVAFVGANLALAWIGPRLTSRLPK
                     EHTLHAPEALGVIYARDFSGLFESHALSRPKRPGEVRIVISGNCMVEGVEARKSLHRA
                     VTARCPRAELYWLAWGALSVSDQAHFLERSLAYRPDVIVWAMAMRDCRSARGVFVDEV
                     PALAVRYGSGPVAAARNLAYLNWDNGWNVVHNLYGYIVPGERYLGVLQLFAPHVIPVP
                     PPAMRMEADARARTSIPVHPPSRYPVSQAAKTLDQLGPRIQTAGCRLVVAILPAAYAD
                     ESFGPGALQHFRSSLSSWCEAHGAVLADLSGVLPPESFADDVHFGRQHMGLVYDRLAQ
                     AMVPTLECVCR"
     gene            1304..1960
                     /locus_tag="PLQ54_15785"
     CDS             1304..1960
                     /locus_tag="PLQ54_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94781.1"
                     /translation="MMLEHFLFATRVLLRTAKGTLHIVFALAVVASFWRVRAWQLSVY
                     GAMVAAIVSRWRWTTGVENELSDAGAMAVQFAVPLLCVLVTCGALAVARRVPGRRRAI
                     IACAIGAVMALWTVCKLSVLREHVPALSQALGTAGMPVMGLSYVVLKLVHLLADAAGP
                     RPPEARATTVMAMVFFPPTYASGPVHRYDEFARSFDALPGARARDWATAIRRITWGTL
                     "
BASE COUNT          276 a          694 c          645 g          370 t
ORIGIN      
        1 cgtggttccc cccatggcgc ccctccgcaa ccaggcggat gggccactgg agccccaagt
       61 taccgccaat ggggagacgg ggtggcagcg tcgggcgcag accgaaccgg ccccggtagg
      121 agtcgcatta ccgaagccgc gccgctcggg tgccgccaac ggggggatgg ttgtgaggct
      181 cctgggacac cccgcagtgg aagcgatcct gttggtcctc atctgggtgc tgtgtctcat
      241 gtaccagaag gccgacccgg ccaacgtcta tgcgcgcttc cagagacgtc acccaagttc
      301 agcgcgatgt ggccctcgtg cgcatctggt gcgtggcctt cgtgggagcc aacctggcgc
      361 tcgcctggat cggcccgcgg ctgacctcac ggctgcccaa ggaacacacg ctgcatgccc
      421 ccgaggcgct cggagtgatc tacgctcgcg acttcagtgg gctctttgag agccacgcgc
      481 tctcgcggcc caagcgcccc ggtgaagtcc gtatcgtcat ttccggcaac tgtatggtgg
      541 agggagtcga ggcgagaaag agcctccacc gggccgtgac cgcccgctgc cctcgggctg
      601 agctctactg gctcgcatgg ggcgcgctct cggtgtcaga ccaggcccac tttctcgaac
      661 ggtccctcgc ctaccgcccc gacgtcattg tctgggctat ggcgatgcgc gattgccgtt
      721 ccgcccgcgg cgtgttcgtc gacgaggttc ccgccctcgc cgtccgctat gggagcggcc
      781 ccgttgccgc ggcccggaat ctggcctatc tgaactggga caacggctgg aatgtcgtgc
      841 acaacctgta cggctacatc gtgcccggcg agcgctacct gggggtcctg cagctcttcg
      901 ctccacacgt catcccggta cctcctcccg ccatgcggat ggaggctgac gcgagagccc
      961 gaacctcgat accggtccac ccgccttccc gatacccggt cagccaagcg gccaagacgc
     1021 ttgaccaact cggtccccgt atccagacgg cggggtgcag gctggtcgtc gcgatcctcc
     1081 ccgcggccta cgccgatgag agcttcgggc cgggggcgct ccagcacttt cggtcttcct
     1141 tgagctcgtg gtgcgaggcc cacggggccg tcctggcaga cctctctggc gtcctcccgc
     1201 cggagagctt cgcggacgac gtgcactttg gtcgccagca catgggtctc gtctatgacc
     1261 gcctcgccca agcgatggtg cccacactgg agtgtgtctg ccggtgatgt tggagcactt
     1321 cctgttcgcg acgagggtgc tcctccggac tgccaagggc acgcttcaca tcgtgttcgc
     1381 cctcgccgtg gtggcctcgt tctggcgagt acgcgcgtgg cagttgtctg tctacggcgc
     1441 catggtcgcg gctatcgtct cacgctggcg atggaccacc ggggtcgaga acgagctttc
     1501 agacgcgggc gccatggccg tccagttcgc cgtgccactg ctgtgtgttc tcgtgacgtg
     1561 tggcgcgtta gccgtcgcgc gacgggtacc cggtcggcgg cgggccatca ttgcgtgcgc
     1621 catcggcgcc gtgatggcat tgtggaccgt gtgcaaactc tcggtgctcc gcgaacacgt
     1681 tcccgccctg tcccaggcac tgggcaccgc cggcatgccc gtgatggggc tatcgtatgt
     1741 ggtcctcaag ctcgtgcatc tgctggccga cgccgcagga cccaggccgc ctgaggccag
     1801 ggccaccacc gtcatggcga tggtcttctt tccccccaca tacgcgagcg ggcccgtgca
     1861 ccgctacgac gagttcgctc ggagcttcga cgctcttccg ggcgctcgag cgcgcgactg
     1921 ggccacggcc atccggcgca tcacctgggg caccctctag gtgtcggtcc tcgccgctca
     1981 cctgg
//
LOCUS       DAOSPL010000969         1984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_57162_length_1984_cov_2.771384, whole genome shotgun sequence.
ACCESSION   DAOSPL010000969 DAOSPL010000000
VERSION     DAOSPL010000969.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57162_length_1984_cov_2.771384"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..456
                     /locus_tag="PLQ54_15790"
     CDS             115..456
                     /locus_tag="PLQ54_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOS94782.1"
                     /translation="MTDLAAALEIVGARWALLIVERLLDGPQRYGDLQRDLGLGTNML
                     AVRLRELEAAGVLYRLPLRHNTRAYVLTECGLALRTPIAALTQWVSQEEPVPGVEHVE
                     DEVGGSAHDKT"
     gene            640..801
                     /locus_tag="PLQ54_15795"
     CDS             640..801
                     /locus_tag="PLQ54_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94783.1"
                     /translation="MLDEVKAYRTYNQQAELLAHRGMDVGDRNRAAGVLRRVNCYRLR
                     GTAPSPRSP"
     gene            complement(907..1233)
                     /locus_tag="PLQ54_15800"
     CDS             complement(907..1233)
                     /locus_tag="PLQ54_15800"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOS94784.1"
                     /translation="MRRGDIVTVAGGVYASKPRPALVVQDDRFDATDSLTVCPLTTTD
                     VEAPLLRVAVPANAETGLSQDSYVMVDKITTVRRSNAHAVLGRLDGTTLVAFERRILV
                     FLGFGA"
     gene            complement(1230..1433)
                     /locus_tag="PLQ54_15805"
     CDS             complement(1230..1433)
                     /locus_tag="PLQ54_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin MazE family protein"
                     /protein_id="HOS94785.1"
                     /translation="MAVRHRVSAYRERMRAQGLRPVQIWVPDVRSPEFAAEAHRQSVL
                     VASSASAIEDMDFVEAVSEDWAE"
     gene            1534..1746
                     /locus_tag="PLQ54_15810"
     CDS             1534..1746
                     /locus_tag="PLQ54_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOS94786.1"
                     /translation="MKTTLYLPDDIKRAVEVEARRRGVSEAEVVRDALRSTLVKTRIE
                     PRGALFHGREPIADRVDELLAEGFGQ"
     gene            1743..>1984
                     /locus_tag="PLQ54_15815"
     CDS             1743..>1984
                     /locus_tag="PLQ54_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015289163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOS94787.1"
                     /translation="MIVDTSALLAYFDASEPAHKAVSAAIESSDELLVVSPYVLAELD
                     YLVLSRHGIQAQQAVLAELGAGAWELATMERERLVQ"
BASE COUNT          365 a          649 c          636 g          334 t
ORIGIN      
        1 tgggcggatc ggtgacaccg acgactaccc cttcatgtat cagcgccagt tcgacgatct
       61 cgacggatac cactactcgc cgtactggat gaagccggat cccgacccgg cgccgtgacc
      121 gacctggctg cggcgctcga gatcgtcgga gcacggtggg cactgctcat cgtggagcgg
      181 ctgctcgatg gtccgcaacg ctacggcgac ttgcagcgcg atctgggcct cggtacgaac
      241 atgctggccg tcaggttgcg cgagctcgaa gccgccggtg tcctctatcg gctgcctctt
      301 cgtcacaaca cgagggcgta tgtcttgacc gagtgcgggc tcgccttgcg cacgccgatc
      361 gccgccctca cacagtgggt cagccaggaa gaacccgtgc ctggtgtcga gcatgtcgaa
      421 gacgaagttg gcggatcagc acacgacaag acgtaactcc ccatgaccac caacaagaac
      481 gaggataacc cggcccggga ggcgcagggc tgagcaatgg caccgacacc tccctccgaa
      541 ccgtgccctt ccgtgccgcg ctagccttgg ttgatcacga ccggttccgc ctgctgcgac
      601 aggtccaggg ccggtcttca ctttctcgag gctcgcctca tgctcgatga ggtgaaggcg
      661 taccggacgt acaaccagca ggccgagctg ctcgcccacc gcggaatgga cgtaggggac
      721 cgcaaccgcg ctgctggcgt gctccgccgc gtgaactgct accggctgcg tggaaccgca
      781 ccttcgcccc gctcaccctg atccagttcc tcctcaaccg gctcgaactc ggccgcacga
      841 ggggctaccg gcagtgggtg aagctcccga tacggccggt caccacctgc gccggcctcc
      901 ttgatgtcag gctccgaagc cgaggaaaac gagaattcga cgttcgaacg ccacgagtgt
      961 ggttccgtcg agtcgtccaa gcacggcgtg ggcattactc cgacggacgg ttgtgatctt
     1021 gtcgaccatc acgtaggagt cttggctcag gccagtctcg gcgttggcgg gaactgcgac
     1081 gcggagcaag ggggcttcca cgtcggtcgt cgtgagggga catacggtga gtgaatctgt
     1141 ggcatcgaac cgatcgtcct ggaccaccaa agccggacga ggcttgctcg cgtagacgcc
     1201 accggcaacg gtgacgatgt caccgcgcct cattcggccc agtcttcgga gacggcttcg
     1261 acgaagtcca tatcctcaat ggcgctcgca gacgacgcga caagcacaga ctggcggtgg
     1321 gcttccgcgg cgaattcagg cgagcgcaca tcaggcaccc agatctgcac cgggcgcaaa
     1381 ccctgagcac gcatccgctc gcggtatgca ctcactctgt gccgtacggc catgttgatc
     1441 acgttacatg taacgatccc ctcatggcaa cgggcgcccg cacatcgcgg gggttcagcg
     1501 cggaggtcta ccaccgcagg taccatataa ggcatgaaga ccaccctgta cctgcccgac
     1561 gacatcaagc gtgccgtcga ggtcgaggcg cgacgacggg gtgtgtcgga ggcggaagtg
     1621 gtgcgtgatg cgctgcgcag caccctggtc aagacccgga ttgagccgcg gggcgccctg
     1681 ttccacggcc gcgaaccgat cgccgaccga gtcgacgaac tcctggcgga gggcttcggc
     1741 cagtgatcgt cgacaccagc gccttgctgg cctatttcga cgccagtgag ccggcgcata
     1801 aggccgtgtc ggcggccatc gagtccagcg atgagctgct cgtcgtgtcg ccttacgtcc
     1861 tggccgagct cgactacctg gtgctgagcc gacacggcat acaggcgcag caggcggtgc
     1921 tcgccgagtt gggcgcaggc gcgtgggagt tggccaccat ggagcgcgag cgactcgtcc
     1981 agga
//
LOCUS       DAOSPL010000970         1982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_57227_length_1982_cov_3.865594, whole genome shotgun sequence.
ACCESSION   DAOSPL010000970 DAOSPL010000000
VERSION     DAOSPL010000970.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57227_length_1982_cov_3.865594"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /gene="hemC"
                     /locus_tag="PLQ54_15820"
     CDS             <1..1132
                     /gene="hemC"
                     /locus_tag="PLQ54_15820"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOS94788.1"
                     /translation="PEPLPSVTLQQPGGAVGIPQFSERLTGSRRSVNWACARPCPIDS
                     AALAPAAVECPRGASGAVRGRAAIREPLRIGARGSRLSRAQAESVAAELRAAHSSLAI
                     ELVWLTTTGDRDTQTALSAMGAQGVFAKEIEAALLEGRIDVAVHSLKDLPTAATPGLA
                     VVAVPKRADPRDALVTRDGSVLRALPPFARVGTGSTRRAAQLRHLRPDVEVVPIRGNV
                     DTRLGKLEAHGLNAVLLAMAGLERTGLRDGRVSPLEAEWFPHAPGQGALGLQCREDDS
                     VTQSLLEPIDDPVSRRTAEAERAALAYLGGGCQAPFGVCSRCGAGTIDLLGCVTSSEG
                     RLQVRRWAGGHMDDPDGLGRELAEALLAAGAGELLSRERSTG"
     gene            1129..>1982
                     /gene="cobA"
                     /locus_tag="PLQ54_15825"
     CDS             1129..>1982
                     /gene="cobA"
                     /locus_tag="PLQ54_15825"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOS94789.1"
                     /translation="MTDRVGRVYLVGAGPGHPGLITVRGLECIRAADCIVYDALVGRE
                     LLAEARQDAELCFVGKRGGHPSMPQDQINALLVEKALVHARVCRLKGGDPFVFGRGGE
                     EALHLRAHGVPFDVVPGVTSAVAAPAYAGIPVTHRGLATSVAFITGHEDPAKEESSID
                     WARLACGVDTLVILMGVSRLRALVALLAENGRDPGTPVALVQWGTTPDQVTVTGTLET
                     IADVAEAAQIGAPVAMVVGDVVSLRNQLAWAERYPLFGRRIVVTRARPQASELSRLIR
                     EQGGVAAEA"
BASE COUNT          270 a          672 c          708 g          332 t
ORIGIN      
        1 tcccgagcca ctgccttcag ttaccctcca acagcccgga ggagccgtgg gtatcccaca
       61 gttcagtgag agactgacag gcagccgccg gagcgtcaac tgggcgtgtg cgaggccatg
      121 ccccatcgac tctgcagcgc tggcgccggc tgcggtagaa tgcccgaggg gcgcctcggg
      181 cgccgtgaga ggtcgtgccg ccatcaggga accactccgc atcggggctc gcggcagccg
      241 tctgtcgagg gcccaagccg agtccgtcgc tgctgagctg cgcgcggctc attcgtcgct
      301 cgccatcgaa ctggtgtggc tgaccaccac tggcgaccgg gacacgcaga ccgccctgag
      361 tgccatgggt gcccagggcg ttttcgccaa ggagatcgag gccgcactgc tcgagggccg
      421 gatcgacgtg gccgtccaca gcctgaagga cctgcccacc gcggcgacgc cgggtctagc
      481 ggttgtcgcg gtgccgaagc gcgccgaccc tcgcgatgcg ctcgtcactc gcgatggatc
      541 ggtgctgcgg gcgctgccgc ctttcgcccg cgttggcacc ggaagcacgc gccgggccgc
      601 ccaactcagg catcttcggc cggatgttga ggtcgtgccc atccgcggga atgtcgatac
      661 gcggctgggt aagctcgaag cccacgggct caacgcggtg cttctcgcca tggccgggct
      721 cgagcgcacg gggctccgcg atggccgtgt ctcgcccctc gaagccgagt ggttccctca
      781 cgcgcccggc cagggtgctc tcgggttgca gtgccgcgag gacgactcgg tcacgcaatc
      841 gcttctggag cccatcgatg atccggtgag ccgccggact gccgaagcgg agcgggccgc
      901 tctggcgtat cttgggggag ggtgtcaggc cccctttgga gtgtgttcgc ggtgcggcgc
      961 gggcaccatt gacctgctgg gctgcgttac ctcctctgag ggccgcctcc aagtccgccg
     1021 atgggctggc ggacacatgg acgatcccga cggcctgggg cgtgaactgg ccgaggcttt
     1081 gcttgcggcc ggcgctggcg agttgctgag ccgagagagg agcactgggt gaccgatcgc
     1141 gtcggccgcg tctacctcgt gggcgccgga cccggccatc cgggtctcat tacggtccga
     1201 ggcctcgaat gcatccgcgc ggcggactgc atcgtctacg acgctctcgt gggccgcgag
     1261 ctcctggctg aggcacgcca ggacgctgag ctctgtttcg tcggcaaacg aggcgggcac
     1321 ccatcgatgc cgcaggacca gatcaacgcg ctactggtcg agaaggcgct ggtccacgcc
     1381 agggtgtgcc ggctgaaggg cggtgatccg tttgtcttcg gtcgcggggg cgaggaggcc
     1441 ctgcacctgc gggctcacgg cgtgcccttc gatgtggtgc ctggtgtcac ttcggccgtc
     1501 gcggcgccgg cctatgcggg tatcccggtc acccatcgcg ggttggcgac cagcgtcgcc
     1561 ttcatcaccg ggcacgagga tccggccaaa gaggagtcct cgatcgactg ggcccgactg
     1621 gcctgcggcg tcgatacgct cgtcatcctc atgggtgtga gccggctgcg ggccctcgtg
     1681 gcgctcctcg cagagaacgg tcgcgatccc ggtacacccg tcgcgctggt gcagtggggc
     1741 accacgcccg atcaggtcac tgtgaccggg acgctggaga ctattgctga cgttgccgag
     1801 gccgcgcaga tcggcgcccc cgtggccatg gtcgttggcg atgtcgtctc gctgcggaat
     1861 cagctggcct gggcggagcg ctacccgctg ttcggccgcc gcatcgtggt cactcgcgcc
     1921 cgcccccagg ccagtgagct ctcgcggctc atccgcgagc agggcggcgt cgccgccgag
     1981 gc
//
LOCUS       DAOSPL010000971         1982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_57244_length_1982_cov_2.875454, whole genome shotgun sequence.
ACCESSION   DAOSPL010000971 DAOSPL010000000
VERSION     DAOSPL010000971.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57244_length_1982_cov_2.875454"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..1394)
                     /locus_tag="PLQ54_15830"
     CDS             complement(207..1394)
                     /locus_tag="PLQ54_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS94790.1"
                     /translation="MNLEEIEAKARESVNPSAISKRSGTSGMTLRERFRRVMQYQTVD
                     RIPNFEFGYWDETLPLWHEQGLPREIDNEAKAYDYFGIENWGVAPIDLGLRPGFPGAL
                     IEETDEYVIAVDGNKATYKINKKGFKSIPHYIDFGLKTRADWEEFKERLQPGPEGRYP
                     DNWDEIAAAYNQRDYPLAVPVGSMIGVPRNWIGFENIALMCYDDPELLEEIVETLCVV
                     VETTIERALRDVEFDFAAGWEDICFNSGPVISPSIYRAIVMPRYRRIADLCNRHGVHV
                     IWTDCDGNLKPIADILLDGGFNCLFPAEVRGGTDPVELREKYGKRQLIQGGVDKHKLA
                     GTFREIEEEMERLLPVVEEGGFIPHVDHRVPADVPLANYKYYLKLKREMFRAGVKEPQ
                     CEL"
     gene            1585..>1982
                     /locus_tag="PLQ54_15835"
     CDS             1585..>1982
                     /locus_tag="PLQ54_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94791.1"
                     /translation="MTTRAVAIGLACVALMALVIPYADLLVRGSWFGLTAFPIGSLLA
                     LLVVVLIVGVALRLAQRGLTPPELALIASMVLVGAGIPSFGLTGLLLPYLAGPTYFMT
                     QERPDDSFLPLLPRWAMVQGHVPAGERISTP"
BASE COUNT          288 a          622 c          687 g          385 t
ORIGIN      
        1 gaagcgtggg tcttggcggg gacggacacg ttgcggccgc tcggcgccgt taccatgatg
       61 ggagggcggt cgaggatctc gccctcccac caggccatga gccgtgcttt ggcttgctcg
      121 aagtcgggct tgagctcgta cacggctcct ccttcggcga gggtcagtgg aggcggtggc
      181 agtgccgggt ctccaccttg gagcctctac agctcgcact ggggttcctt gaccccggca
      241 cggaacatct cgcgcttcag cttcagatag tatttgtagt tggccagagg cacatcggcg
      301 ggcacccggt gatcgacgtg ggggatgaag ccgccctcct cgaccacggg cagcaggcgc
      361 tccatctcct cctcgatctc gcggaaggtc ccggccaact tgtgcttatc gacgccgccc
      421 tggatgagct gccgcttgcc atacttctcg cgcagctcga caggatcggt gccgccgcgc
      481 acttcggcgg ggaacaggca gttgaagccg ccgtcgagca ggatgtcggc gatgggcttg
      541 aggttgccgt cgcagtcggt ccagatgaca tggacgccgt ggcggttgca caggtcggcg
      601 attcggcggt agcgcggcat cacgatcgcc cggtagatgc tcggcgagat aacggggccg
      661 ctgttgaagc agatatcctc ccagccggcg gcgaagtcga actcgacgtc gcgtagcgcg
      721 cgctcgatgg tggtctcgac gacgacacac agggtctcaa cgatctcctc gagcaactcg
      781 ggatcgtcgt agcacatgag ggcgatgttc tcgaagccga tccagttccg cgggacaccg
      841 atcatgctgc ccacgggcac ggccaggggg tagtcgcgct gattgtaggc ggcggcaatc
      901 tcgtcccagt tgtcggggta gcggccctcg gggcccggct ggaggcgttc cttgaactcc
      961 tcccagtcgg ctcgggtctt gaggccgaag tcgatgtagt gcgggatgga cttgaaaccc
     1021 ttcttattga tcttgtaggt tgccttgttg ccgtcgaccg cgatgacgta ctcgtccgtc
     1081 tcctcgatga gagcacccgg gaagccgggg cggagtccga ggtcgatggg cgccacgccc
     1141 cagttctcga tgccgaagta gtcataggct ttggcctcgt tgtcgatctc gcgcggcagg
     1201 ccctgctcgt gccagagcgg cagcgtctca tcccagtagc cgaactcgaa gttcgggatg
     1261 cgatcgacgg tctggtactg catcacgcgc cggaaacgct cccgcagggt catgccggag
     1321 gtgccggagc gcttggagat cgcgctgggg ttgacggact cgcgggcctt ggcctcgatc
     1381 tcttccaggt tcatcgggtg tgggtcctct cgaggggaac gtggctggcg aatggctcgg
     1441 cgcgccgtgg cgccggcgcc taggctacag gagcggctga cggggttcaa ggggtgcgcg
     1501 atgcccctgt gaggtgagtc gccagctccc gtcgaacacg gccttcgcga ttcccgagca
     1561 gggcatccct ctgaggcagg cgcgatgacc accagggcgg tcgcaatcgg gcttgcgtgc
     1621 gtggcgctga tggcgctggt catcccgtac gcggacctgc tggtccgcgg gagctggttc
     1681 gggctgaccg ccttccccat cggttcgctg ttggcgttgc tggttgtggt cctgatcgtc
     1741 ggcgtggcac tcaggctggc ccagcgcggg ctgacgccgc ccgagctggc gctcatcgcc
     1801 tccatggtgc tcgtcggcgc cggcataccg tcgttcggcc ttacgggctt gctgctgccc
     1861 tatctggccg ggccgaccta tttcatgacc caggaacggc cggacgacag cttcctccct
     1921 ctgctgccac gctgggcgat ggttcagggc cacgtccccg cgggcgagcg catcagcacc
     1981 cc
//
LOCUS       DAOSPL010000972         1982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_57255_length_1982_cov_2.244940, whole genome shotgun sequence.
ACCESSION   DAOSPL010000972 DAOSPL010000000
VERSION     DAOSPL010000972.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57255_length_1982_cov_2.244940"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1982
                     /locus_tag="PLQ54_15840"
     CDS             <1..>1982
                     /locus_tag="PLQ54_15840"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOS94792.1"
                     /translation="ELQILTAAEAVTPDRVEPLSLDRIGRASRFRLLHQAFLMQLSPP
                     DDVLIAPRASAVRYEPYQYVPAYRALRMPRPRLLIADDTGLGKTIEAGIVLRELTARR
                     RAGRVLVICPASIMSQWQDELLRKFGMRFDILDRDGLYETRRKHEAGTNPWSACGRGI
                     ASMDFVKRREGAFQELSASRWDVVIVDEAHHLAGGADDKDDVTDRHRLGRWLSEAADA
                     LLFLTATPHDGYDERFASLLNLLDPTLAPGGKLEFRRYSRSLVRRLKGHIRDENGQPK
                     FVPRAPVEPVAVELSPEEKALHQRVRNQAKDLDEMAAQVAKRRRAVDAEAIRLVATVL
                     RKRAASSRAALKATLENRIGNLEEAHVKAELRRDHLRALKRGDTVPDEALKDLERDAH
                     QNYLSVVRGYGKDLRRYEDERAAVEDLLDLLLNCWGSPESKMVALTAWLSRVPPPDKV
                     IIFSEYADTVAAIETELEGHAEWAGQSVVLTGTLSREQRTAVIREWAGPGKRFLIATD
                     AASEGLNLHHCCHRVLHFELPWNPNRLEQRNGRVDRYGQTEAPQIGFLYAANTYEGEV
                     LARLLTKIENQVRRLGSVGDVLGPMQVSSIVALLERTPPDELAAIDHADREIDNALNV
                     RAATGLRQHLGDGTVDEAETDAVRDAVARSHPEWLD"
BASE COUNT          341 a          712 c          645 g          284 t
ORIGIN      
        1 ccgagctaca gatactgaca gcagccgagg ccgtcacgcc cgatcgagta gagcccctgt
       61 cgctcgaccg catcggccgg gcgagtcgct tccgcctgct ccatcaggcc ttcctcatgc
      121 agctctcgcc gcccgacgac gtcctcatcg cgccacgggc ctccgcagtg cgctacgagc
      181 cctaccagta cgtccccgcc taccgggccc tgcggatgcc tcggccccgc ctgctcatcg
      241 ccgatgacac cggtctcggc aagaccatcg aagccggcat cgtcttgcgc gagcttaccg
      301 ccaggcggcg agccgggcgc gtactcgtca tctgccccgc gagcatcatg tcgcagtggc
      361 aagacgagct gctccgcaag ttcggtatgc ggttcgacat cctcgatcgc gacggcctct
      421 atgagacacg gcgcaagcac gaagccggta ccaacccctg gtcggcctgc ggccggggca
      481 tcgcgtccat ggacttcgtg aagcgccgcg aaggcgcgtt ccaggagctc tctgcgtctc
      541 ggtgggacgt ggtcatcgtc gatgaggctc accacctcgc cggcggtgcg gacgacaagg
      601 acgacgtcac cgatcggcac cggctcggcc gatggctctc cgaggcggct gacgctctcc
      661 tgttcctcac cgcgacgcca cacgatggct acgacgagcg cttcgcgtcg ctgctgaacc
      721 tgctagaccc gaccctggct cccggcggca agctggagtt ccgccgctac agccgtagcc
      781 tggtccggcg cctgaagggg cacatccgcg acgagaacgg tcagcccaag ttcgtcccgc
      841 gcgccccggt ggaacccgtc gcagtcgagc tcagcccgga ggagaaggca ctccaccagc
      901 gggtccgcaa ccaggccaag gacctggatg agatggccgc tcaggtcgcg aaacgacgtc
      961 gtgccgtgga cgccgaggcc atccggctgg tcgccaccgt cctccgcaag cgggccgcgt
     1021 cttcccgcgc cgcgctgaag gccacactcg agaaccgcat cgggaatctg gaggaagccc
     1081 acgtcaaggc cgaactgcgc cgcgaccatc tccgtgccct caagcgaggc gacacggtgc
     1141 ccgacgaggc gctcaaggac ttggagcgcg acgcccacca gaactacctc tctgtggtgc
     1201 ggggctacgg gaaggacctg cgccggtacg aggacgagcg cgccgccgtc gaggacttgc
     1261 tcgacctgct gctgaactgc tggggctcgc ccgaatcgaa gatggtggcg ctgacggcgt
     1321 ggctctcgcg cgttccgcca cccgacaagg tcatcatctt ctccgaatac gccgacacgg
     1381 tcgccgccat cgagacggag ctcgagggcc acgccgagtg ggccggacag tcggtggtgc
     1441 tgacgggcac gctgtcgcgc gagcagcgga cggctgtgat tcgcgagtgg gcgggacccg
     1501 ggaagcgctt cctgatcgcc accgacgccg caagcgaggg cctgaacctg caccactgct
     1561 gccaccgggt gctccacttc gagctgccgt ggaacccgaa ccgcctcgag cagcgcaatg
     1621 gccgcgtcga ccgatacggg cagacggagg caccccagat cgggttcctc tacgccgcca
     1681 acacgtacga gggtgaagtg ctagcgcgcc tgctcacgaa gatcgagaac caagtcaggc
     1741 ggctgggctc cgtcggcgat gtgctcgggc caatgcaggt cagcagcatc gtcgctctgc
     1801 tcgagcgcac gccgcccgac gagctggcgg ccatcgacca tgccgatcgc gagatcgaca
     1861 acgcactgaa cgtgcgcgcc gcaactggcc tgcgccagca cctgggcgac ggaaccgttg
     1921 acgaggccga gaccgatgcg gttcgcgacg ccgtggccag gtcccacccg gagtggctcg
     1981 ac
//
LOCUS       DAOSPL010000973         1978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_57398_length_1978_cov_3.504940, whole genome shotgun sequence.
ACCESSION   DAOSPL010000973 DAOSPL010000000
VERSION     DAOSPL010000973.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57398_length_1978_cov_3.504940"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..940
                     /locus_tag="PLQ54_15845"
     CDS             59..940
                     /locus_tag="PLQ54_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HOS94793.1"
                     /translation="MTSPSSIDSFPPPEMARRAAEVGVNKAHLPFGPMVLLSVLAGAY
                     IAMGAALYTTAMTGANGIVPFGAARVLGGLAFCLGLILVVVGGAELFTGNMFLVMGWA
                     DRRLPFVLLIRNWIVVYVGNLVGSVAVAYLVYLAQQHTMADGAVGLQALTTASAKCGL
                     PFTVALSRGVLCNALVCLAVWMTYSCRTTGDKILAILFPITAFVTCGFEHSVANMYFI
                     PVALFIKHGGPAAFWATAGASPGQFADLTWANFAGANLLPVTLGNIVGGAGFVAGVYW
                     LIYGRGEGTTQEKGPRR"
     gene            937..>1978
                     /locus_tag="PLQ54_15850"
     CDS             937..>1978
                     /locus_tag="PLQ54_15850"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS94794.1"
                     /translation="MTTRERFLSIVHFETPDYVPYWYPPGIGIAHKETVERWVAEEGY
                     PSDYDSIVEFFGAEGYAGVGLHTGFVPDFPVETEDLGDGYVLIRQHKATTRELANNWD
                     LYTMPEFRTYMFEDRRDFEEHMRPRLDPAHPDRPRPEVPAEQPEQPLAISCPSYFGVY
                     RSWFGLDRISYLMHDDPKLLHEMSRAFTDLFLDQAWEVARKVQVDAIIGWEDMCYRSG
                     MLISPAAFDEFCAPYYREVADFARRIGAAVVDVDCDGDITEFVHCLWRSGVNMCHAFE
                     PTHGGSDVLRIRAELPRFIICGGIDKHATVDPDPAMAIAEVDAKVPPLVRSGGYLPSI
                     DHGLPPICHYRPF"
BASE COUNT          308 a          635 c          652 g          383 t
ORIGIN      
        1 cggtacacga aggcggcgcc gcgcgatgcc gccatgcctc aggcgcccgg aggcctccat
       61 gaccagcccc tcgtccatcg actccttccc tccgccggag atggctcggc gcgcggcgga
      121 agtgggtgtc aacaaggccc atttgccctt cgggcccatg gtgctgctgt cggtcctcgc
      181 gggtgcttac atcgccatgg gggccgccct gtacaccacg gcgatgacgg gggccaatgg
      241 catcgtcccc tttggggcgg ctcgagtcct cggcggcctc gcattctgtc tgggcctgat
      301 tctggttgtg gtcggcgggg ccgagctgtt cacggggaac atgttcctgg tcatgggctg
      361 ggccgaccgt cggctaccct tcgtgttact cattcggaac tggatcgttg tgtacgtcgg
      421 gaacctggtg ggctcggtgg ctgtggccta tctggtctat ctggctcagc agcacacgat
      481 ggccgacgga gctgtcgggc tccaggcgct gaccacggcc agcgccaagt gcggactccc
      541 gttcacggtg gccctctcgc gtggagtgct gtgcaatgcc cttgtctgcc tcgccgtgtg
      601 gatgacgtat tcctgccgca ccacaggtga caagattctg gcgattctgt ttcccatcac
      661 cgcgttcgtg acatgtggct tcgagcacag cgtggcgaac atgtacttca tccccgtggc
      721 cctgttcatc aagcacgggg gcccggcggc tttctgggcc acggccggcg cgtcacccgg
      781 gcagtttgcc gacctgacct gggcgaactt cgccggggcc aatctgctgc ccgtgactct
      841 ggggaacatc gtcgggggtg ccgggttcgt ggccggggtg tactggctga tctatggtag
      901 aggcgagggc acgacacaag agaaggggcc gcgccgatga cgacgcgcga acggtttctg
      961 tcgatcgtcc acttcgagac acccgactac gtgccctact ggtacccgcc gggcatcggc
     1021 atcgcccaca aggagacggt cgagcggtgg gtcgccgaag agggctaccc gagtgactac
     1081 gattcgatcg tcgagttctt tggtgccgaa ggttatgcgg gagtcggact tcacacgggg
     1141 ttcgttcctg actttcccgt cgagaccgag gatctgggcg acggctatgt gctgatccgc
     1201 cagcacaagg cgaccacgcg cgaactggcc aacaactggg atctgtacac catgcctgag
     1261 ttccggacct acatgttcga ggatcgccgc gacttcgagg agcacatgcg gccgcggctc
     1321 gacccggcgc atcctgaccg tccgaggccc gaggtcccgg ccgagcagcc cgagcaaccg
     1381 ctggccatca gttgcccgag ctactttgga gtctatcgga gctggttcgg gctcgaccgc
     1441 atatcctacc tcatgcacga cgacccgaag ctgctccacg agatgagccg cgcgttcacc
     1501 gacctgttcc tggatcaggc atgggaggtg gcacggaagg tgcaggtgga cgccatcatc
     1561 ggctgggagg acatgtgcta ccgcagtggc atgctcattt cgccggcggc cttcgacgag
     1621 ttctgcgcac cctactaccg cgaggttgcc gacttcgccc gccgcatcgg cgcggccgtg
     1681 gtcgatgtgg actgcgatgg cgatatcacg gagttcgtcc actgcctgtg gcgctcgggt
     1741 gtgaacatgt gccacgcctt cgagccgaca cacggcggct ccgacgtgct acggatccgg
     1801 gcggagctgc cccgattcat catctgcggt gggatcgaca agcacgccac ggtggacccg
     1861 gacccggcta tggccattgc tgaggtggac gcgaaggttc cgccactggt gcggagcggc
     1921 ggctatctgc cgagcatcga ccacggtctg ccacccatct gccactaccg gcccttct
//
LOCUS       DAOSPL010000974         1976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_57498_length_1976_cov_2.762103, whole genome shotgun sequence.
ACCESSION   DAOSPL010000974 DAOSPL010000000
VERSION     DAOSPL010000974.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57498_length_1976_cov_2.762103"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1645
                     /locus_tag="PLQ54_15855"
     CDS             <1..1645
                     /locus_tag="PLQ54_15855"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HOS94795.1"
                     /translation="LRFRRHASRVAEVAAALIRHGWHPALRYLGLDRFRRRDQSEDET
                     ALPASSLRAMLSELGPTYVKVGQLLSTRPDLIPPAYVEELEKLQDQGEPIPLDQVRAV
                     LREELGDPDEAFEDFDPQPLAAASLGQVHRARLRGVGAVIVKVQRPGAREVVETDLEI
                     LFQLARALQAASSRARRIDVVSVVEDVASLLRSELDYTAEGRNADRLRSQLAPGAPVR
                     IPKVHWEHTTRRVLVMECLTGCKVTDLGALRAMGLSPERIAEHLASVLLRQIFIDGVF
                     HGDPHPGNVQAFRDGSIGFVDFGVVGHLDGASRKRLLEMLTAIYRGEGEAFAEHLLAL
                     CYRNEHVNRTAFSQDAEAVVREFLALPPEEGRLSVALRNALRIVLKHSLSLPPAVPLL
                     VRCVVSFEGVCKALDPDFEILDLAARMLFEAAASRLNPRSLGVSAMLAGADAVDLLLN
                     SPRMVNDILSKMATGRISVNVEHRGLRDFADRLDKIANRLAYALMVAAALLSSSMLIQ
                     LNEGPAFYGLPIVGLAAFVLCFVLGVELLWTILRSGKLR"
     gene            1721..>1976
                     /locus_tag="PLQ54_15860"
     CDS             1721..>1976
                     /locus_tag="PLQ54_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94796.1"
                     /translation="MALESSGTRKRASIISHTHWDREWYLPFQGFRMRLVGLIDEMLD
                     VMERDPRYRYFHMDGQAVVLEDYLEIRPENRGRLERLIRGG"
BASE COUNT          281 a          637 c          680 g          378 t
ORIGIN      
        1 gttgcggttt cgccgccatg cttcccgggt tgctgaagtc gcggcagcac tgatccgcca
       61 cgggtggcat cccgctctgc gctatctggg gctggaccgg ttccggcggc gggaccagag
      121 cgaagacgag accgcgctac ctgccagctc tctgagggcc atgctcagcg agctgggtcc
      181 cacgtacgtg aaggtcggcc agttgctcag cacccgtccc gatctcattc ctccggccta
      241 cgtggaagag ctggagaagc tccaggacca gggcgagccc attcccctgg atcaggttcg
      301 ggcggtgctg cgcgaggagc tgggcgatcc ggacgaggcg tttgaggact tcgatcccca
      361 gcctctggcg gcagcgtcgc tgggccaggt gcatcgggcg cggctccgcg gcgtcggcgc
      421 ggtgattgtg aaggtccaga gaccgggcgc ccgggaggtt gttgagaccg atctcgagat
      481 cctcttccag ctcgcccgtg ccctgcaggc cgcttcctcc cgagcgcgcc ggatcgatgt
      541 ggtgagtgtg gttgaggatg tcgcctcact cctgcgtagt gagcttgact acacggctga
      601 ggggcggaac gcggaccggt tgcgcagtca gttggctccg ggcgcaccgg tgcggatccc
      661 caaggtgcac tgggagcaca cgacgcgccg ggtcctggtc atggagtgtc tgaccggatg
      721 caaggtgacg gatctgggtg cgctgagggc gatggggctc tcccccgagc ggatcgccga
      781 gcatctggca tcggtgctct tgcgccagat cttcatcgac ggcgtgttcc atggcgaccc
      841 tcaccccggg aacgtccagg cgttccgcga cgggtccatt gggttcgtcg actttggcgt
      901 ggtgggccac ctggacggcg cctcgcgcaa gcgtctgctc gagatgctga cggccattta
      961 ccggggcgag ggggaggcgt tcgcggagca cctgcttgcc ctttgctacc gcaatgagca
     1021 cgtgaaccgc accgcgttca gtcaggatgc cgaggcggtg gtgcgcgagt ttctggctct
     1081 tccacctgag gagggccgcc tcagcgtggc gctccgcaac gcgctgcgca tcgtgctcaa
     1141 gcacagtctg tcgttgcctc cggcggtgcc gcttctggta cggtgcgtcg tgtcgtttga
     1201 aggcgtgtgc aaggcactcg acccggactt cgagatcctc gacttggcgg cccggatgct
     1261 tttcgaggcc gctgccagcc ggctgaaccc gcgcagcctg ggcgtctcgg ccatgctggc
     1321 cggcgccgac gcggtcgatc tgctgctgaa ctcgccgcgg atggtcaacg acattctgtc
     1381 gaagatggcg acgggtcgga tcagcgtcaa cgtggaacat cggggtctcc gcgacttcgc
     1441 cgaccggctg gacaagatcg ccaaccgcct ggcctacgcc ctcatggtcg ccgccgctct
     1501 gcttagctca tcgatgctca tccagctcaa cgaggggccg gcgttctacg gcctccccat
     1561 cgtggggctc gccgctttcg ttctctgctt cgtgctgggc gtcgagctcc tctggaccat
     1621 cttgcgctcc ggcaaactgc gctagggagg ccgtctcgtc gtctcggccg aaccggggcc
     1681 ccgcgttcgt gtcactctac cggatcgagg cggttcccgc atggcattgg agtcctctgg
     1741 caccaggaag cgcgccagca tcatctcaca cacccactgg gaccgggagt ggtatctgcc
     1801 cttccagggc tttcgcatga ggctcgtggg cctcatcgac gagatgctcg atgtgatgga
     1861 gcgcgacccg cgctaccgct acttccacat ggacgggcag gctgtggtgc tcgaggatta
     1921 cctcgagatc cggcccgaga accgcgggcg gctggagcgc ttgatccggg gcgggc
//
LOCUS       DAOSPL010000975         1972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_57670_length_1972_cov_2.934794, whole genome shotgun sequence.
ACCESSION   DAOSPL010000975 DAOSPL010000000
VERSION     DAOSPL010000975.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57670_length_1972_cov_2.934794"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /locus_tag="PLQ54_15865"
     CDS             complement(<1..107)
                     /locus_tag="PLQ54_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913180.1"
                     /note="regulator of RNase E; increases half-life and
                     abundance of RNAs; interacts with RNase E possibly
                     inhibiting catalytic activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 4-hydroxy-4-methyl-2-oxoglutarate
                     aldolase"
                     /protein_id="HOS94797.1"
                     /translation="MSFVTCDLCDDHPDDVRVCEPMFASFGGRETFCGE"
     gene            complement(144..632)
                     /locus_tag="PLQ54_15870"
     CDS             complement(144..632)
                     /locus_tag="PLQ54_15870"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOS94798.1"
                     /translation="MGVYRVTVRDAAGETRATTVQAADPTAAARAMQARGLFVSQVDL
                     APRVATGDAVGGLHARVFPVSADALALFFRHLAALTKVGQTPAAALASMASRMTDRRL
                     AAFANRASARCADGEPLSAAVCLEPVLFPQWVAAMVAVAQQPATTGTHLYRYKDIQAL
                     LA"
     gene            complement(637..1809)
                     /locus_tag="PLQ54_15875"
     CDS             complement(637..1809)
                     /locus_tag="PLQ54_15875"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOS94799.1"
                     /translation="MPLFQLETRNEAGQVQTRRVAAESQVAAVRMLRARGEFVVRGQR
                     VTRRRMGDPLRSLRAAVWPIGPRALALLFAQLADLIRAGFSTANALEHLAGQTTERRL
                     ASFCREAAADVARGIPLSEAMALRPDLFESWVSASVAVGEQTGRLDVMASLLEQEFRT
                     QTQFGRRFTVAAIYTRAILVFAVLAATSPAVIHVGLSGWLVSLAEIGGLAYVGWKFLH
                     IISRFAWPRALLAGLSRFVPVCGSLRGSLSRLRAQRALRELIRAGIDPPSAFEFAAPV
                     SGGGSSGRGLRRGADVLRAGGSFGRAYRATGLFGQREAGLMETAEETGELDSMIDETV
                     QSLEDSADHAALRATYWGWGLLILVSVAVTVLAIYLGTSGYVNALLEWFDDMMEGI"
BASE COUNT          339 a          661 c          707 g          265 t
ORIGIN      
        1 atctcgccac aaaaggtttc acgaccacca aagctggcaa acatgggctc gcagacacgc
       61 acatcatctg gatggtcatc acacaaatcg caggtaacaa agctcataac agctcctttt
      121 aagcgttaaa aatattggtg tgtttaggcc aatagtgcct gaatatcttt gtagcggtat
      181 aaatgcgttc ccgtagttgc gggctgctgg gcaacggcca ccatggcggc gacccactga
      241 gggaacagga cgggctcgag gcacaccgcg gccgacagag gctccccgtc ggcgcaccgg
      301 gcggaggcgc ggttcgcgaa ggcggccagg cgccgatcgg tcattcgaga ggccatcgag
      361 gccagggcgg cggcgggcgt ctgcccgacc ttggtcagcg cggccaggtg ccggaagaag
      421 agggccagcg cgtcggccga caccgggaag acccgggcat ggagccctcc caccgcgtcg
      481 ccggtggcta cccgaggcgc caggtcaacc tgtgagacga acagcccgcg ggcctgcatg
      541 gcgcgcgcgg ccgccgtggg gtcggccgcc tggaccgtgg tggccctggt ctcgcccgcg
      601 gcatcccgga ccgtcacgcg gtacacgccc acggagctag atcccctcca tcatgtcatc
      661 gaaccattcc agcagcgcat tgacgtaccc gctcgtgccg aggtagatgg ccaggacggt
      721 gaccgccacg gagaccagga tcagtaagcc ccagccccag taggtggctc gcagcgccgc
      781 gtggtcggcc gagtcctcga gggattggac ggtctcgtcg atcatgctat cgagctcgcc
      841 agtctcctcg gcggtctcca tgagcccggc ctcgcgttgg ccgaagaggc cggtggcgcg
      901 gtaggctctg ccgaagctgc cgccggcccg gagcacgtcg gcccccctgc gcagccctcg
      961 cccggagctg ccgccaccgg agacaggggc ggcgaactcg aaggccgatg ggggatcgat
     1021 gccggccctg atgagctccc gcagggcccg ttgcgccctc aaccgggaca ggctgccgcg
     1081 cagggagccg cacacgggca cgaaccggct gagtccagcg agtagggccc gcggccaggc
     1141 gaaccggctg atgatgtgca ggaacttcca tcccacatag gcgaggccac cgatctcggc
     1201 gaggctcacg agccagcccg agagccccac gtggatgacg gcggggctgg tggcggccaa
     1261 gacggcgaag acgaggatgg cgcgggtgta gatggccgcc acggtgaacc gccggccgaa
     1321 ctgggtctgc gtgcggaact cctgctcgag cagagaggcc atcacatcga gtcggccggt
     1381 ctgttcaccg acggcgacgg aggcggacac ccaggactcg aacagatcgg ggcgaagggc
     1441 catggcctcg gacagcggga tgcctcgggc cacgtcggcg gcggcctccc ggcagaagga
     1501 ggccagccgg cgctcggtgg tctggccggc caggtgttcg agggcattcg cggtgctgaa
     1561 gcccgcgcgg atcaggtcgg ccagttgggc gaagagcagg gccagggcgc gcgggccaat
     1621 gggccacacc gcggcgcgga gcgaacgcag cgggtcgccc atccggcggc gggtcacgcg
     1681 ctggccgcgg acaacgaact cgccgcgggc ccgcagcatc cggacggcgg cgacctgcga
     1741 ctcggccgct accctccggg tctgtacctg gcccgcttca ttgcgcgtct cgagctggaa
     1801 cagcggcatg tgcccgctcc gatcccgcag ggccagtcgc atctgccccg gatgcctgaa
     1861 cccctggagg cgcctcgggg ctcaggtctc cgtcccgctt cctgatgccc gagacgtcgc
     1921 cgaggttggc cagtagggca cggagggggc gtccgtcggg cagggtgagg cc
//
LOCUS       DAOSPL010000976         1972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_57688_length_1972_cov_1.850287, whole genome shotgun sequence.
ACCESSION   DAOSPL010000976 DAOSPL010000000
VERSION     DAOSPL010000976.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57688_length_1972_cov_1.850287"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(872..1225)
                     /locus_tag="PLQ54_15880"
                     /pseudo
     CDS             complement(872..1225)
                     /locus_tag="PLQ54_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002528420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
     gene            1325..1636
                     /locus_tag="PLQ54_15885"
     CDS             1325..1636
                     /locus_tag="PLQ54_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020488039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HOS94800.1"
                     /translation="MWFAGYPEAMSDPMPVSTAGLSEQQASLLEFEKQWWALPGSKES
                     EIRERFDISPTRYYQLLNALIDSEAALAHDPLLVKRLRRLRSSRQKERTARRLGVNLR
                     V"
     gene            complement(1620..>1972)
                     /locus_tag="PLQ54_15890"
     CDS             complement(1620..>1972)
                     /locus_tag="PLQ54_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020488040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94801.1"
                     /translation="VFAEGYRLPLPKASAFTVGNVVLVPGGTAAAVEARQPGTLAHEA
                     AHSWQYFGLLGLPFLPLYAVAAGWSWLRTGDPASANWFERSAGLVRGGYVEHPFDNAG
                     FVRVARAVGITRAG"
BASE COUNT          316 a          668 c          678 g          310 t
ORIGIN      
        1 aggtgcggga tgtgcgcggc gaccgcaggc ggtcgctccc ggcgcacagg gccccgcaca
       61 cggaactcac gccaccgtgg ggccccgtgt cctggacggc ggacacaccc cacggctggc
      121 gatgcagtgg gcacgaagcc cggtgcggga tgcgccattg ccttaggtgc gggatgtgcg
      181 cggcgaccgc aggcggtcgc tcccggcgca cagggccccg cacacggaac tcacgccacc
      241 gtggggcccc gtgtcctgga cggcggacac accccacggc tggcgatgca gtgggcacga
      301 agcccggtgc gggatgcgcc attgccttag gtgcgggatg tgcgcgccgt ccagcgcaca
      361 gggccccgca caccgaacat cgacatcggt ggctgggctt tctgagctga tctctgcgcc
      421 caggggggaa ctgtgcgtgc ctctgcgcat gccgtcacgc cgtgaacttt cagggcccgg
      481 ccagttgctt gtgacgatcg ccgtcgagcc ccggcgaccg gtcttcactt gcccccgcac
      541 ggtcgatctg tgtgtcgtgg agctgtgccg tgccgcgcgt aggggcattt gcgccatacc
      601 ggcgtactgc ttcatgcccg accatgttca catgttcatt caagcgacgc accaggatgc
      661 ggacgcatgg ctcacgatag ttgagttcaa acggacaacg agccaacgcc tctcggatgg
      721 cagtgagatg gtacgatggc agccaggctg ccacgtctcg gtaccggatt ccgccggggg
      781 cgtggatgcg gtatgcgcgt acgtgtggaa caatccggtc cgtgcagggc ttgtgaccga
      841 ctggagagac tacaggttct cgggctctct ggcgctcggg ggagtcggtg gcgatccctg
      901 ctgcgttctc cttggcgaac ccctcttccg cctgctcgcg ggacgggtgc gtctgcagcg
      961 agagggcgtg ccgggcggag agcactttca cgaggtaggg gaagcggtcg ccgaagacag
     1021 ccctgctggc atcgccgatc accgacggag tggctgccac gaccgcatcg agccgctcgc
     1081 cacccacctc ggaaggggcc agcgggtggg cgcccagcca gtactcggcg acgggcctgc
     1141 cgtcggccgg ccggccgagc agcgcgggga tggcatcggt ggttccccac gcgtagtgct
     1201 ggaacgtgcc gtgcagaacc tgcatggagg atcctcatct gttgactgcc aggggtgggg
     1261 gcctcgggcc gcaaccgagt ctatccgagg tgtgggaggc acctgcacaa cctgaatgac
     1321 atcgatgtgg ttcgcgggct atcctgaggc catgtccgat ccgatgcctg tctccaccgc
     1381 gggcctgtcc gaacagcagg cttcgctgct cgaattcgag aagcagtggt gggcgttgcc
     1441 cggctcgaag gaatccgaga tccgcgagcg tttcgacatc tcgccgaccc ggtactacca
     1501 gctgctcaat gcgctgatcg actccgaggc tgccctcgcc cacgacccgc tgctggtcaa
     1561 gcgtctccgg cggttgcggt cctcgcggca gaaggaacgc acggcccgcc ggctgggcgt
     1621 caacctgcgc gtgtaattcc caccgcccgc gcaacgcgga caaagccggc gttgtcgaac
     1681 gggtgttcaa cgtaaccgcc ccgcaccagt cctgcgctgc gctcgaacca gttcgccgag
     1741 gccgggtccc ctgtgcgcag ccacgaccag cccgctgcca ccgcgtacaa gggcaggaac
     1801 ggcagcccga gaagcccgaa gtactgccac gagtgcgccg cctcgtgcgc gagcgtcccc
     1861 ggctggcgcg cctcaaccgc agccgcggtg ccgcccggca ccagcacaac gttgccgacg
     1921 gtgaacgccg aggccttcgg cagcggcaac cggtagccct cggcgaagac aa
//
LOCUS       DAOSPL010000977         1970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_57742_length_1970_cov_3.272063, whole genome shotgun sequence.
ACCESSION   DAOSPL010000977 DAOSPL010000000
VERSION     DAOSPL010000977.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57742_length_1970_cov_3.272063"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..614)
                     /locus_tag="PLQ54_15895"
     CDS             complement(12..614)
                     /locus_tag="PLQ54_15895"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOS94802.1"
                     /translation="MWRDRAARAGRSVQLARSRLHSWTALAALIVARPSHWTDVWELV
                     PIAAGLLLRSWALGHLRKNEELCTSGPYAYTRNPLYLGSALILLGYCLMVNSVWFALL
                     VCLVGVAVRRVVVWREEAELMARFGEQYAAYRRSVPAVIPRWVPYRDGVARPFSWEQA
                     WRNNLAQGWLLVWLFAVMLEAKQEITEAWLGIHYPPAWLS"
     gene            complement(704..955)
                     /locus_tag="PLQ54_15900"
     CDS             complement(704..955)
                     /locus_tag="PLQ54_15900"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOS94803.1"
                     /translation="MAVEDKQIAMRVRREIVRRSLDASQLMIHVAHGHVELGGIIKPA
                     NKNAAIDMAKELEFARERVIRIPGVRDMTWRYLRFFGGA"
     gene            complement(1028..>1970)
                     /locus_tag="PLQ54_15905"
     CDS             complement(1028..>1970)
                     /locus_tag="PLQ54_15905"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOS94804.1"
                     /translation="GGGISGPETGLLGLDYDESYEGPGVKVIGRLYNGPCTKAGSEVA
                     AGEYILQINGKDVQFGESLFDALKGMVGKRVKLTVNSKPEAEGARTISVKPIGGGEWA
                     NLRYEQWVRDNRNRVLELSGGRLYYLHIRSMDGASLERFRRDLFGEAQRYEGLVIDVR
                     YNGGGRIHDDLLEELTKKVHVYENQRNAPAVTQPIRQYDKPKVLIINEHCASDSEIFP
                     NGFREKKLGKIVGVRTYGGVIGTYDITLLNGHGFRIPVTGWYTLNGTNLENYGVPPDI
                     EVPYPYEAYRDGRDPQIEAAVAELLKTVGAPEEPAAP"
BASE COUNT          347 a          686 c          594 g          343 t
ORIGIN      
        1 gcgcggacag gctaggagag ccaggccgga gggtagtgaa tccccaacca agcctccgtg
       61 atctcctgct tcgcctccag catcacggca aacagccaca ccagcagcca tccctgggcg
      121 aggttgtttc gccaggcctg ctcccacgag aacggcctgg ccactccgtc gcggtagggc
      181 acccaccgcg ggatgacggc gggcacgctt cggcggtacg ccgcgtactg ctcaccaaag
      241 cgagccatca gctcggcctc ttcacgccac accacaaccc gtctgaccgc gacacccacc
      301 agacacacga ggagcgcgaa ccagacgctg ttcaccatca ggcagtaccc aagcaggatc
      361 agcgcactgc ccaggtagag cgggttgcgg gtgtacgcat aggggcctga ggtgcagagc
      421 tcttcgttct tccggaggtg gcccagggcc caagaccgca acagcagccc cgcggcaatc
      481 gggacgagct cccacacatc ggtccagtgc gacggccgag ccacaatgag agcggccaac
      541 gccgtccagg agtgcaggcg gctccttgcg agctgaaccg agcggcccgc ccgggcggcg
      601 cggtctcgcc acacatgcga accattcccc agggaacgtt gacggtcttc catcatgcca
      661 atacagtcca cgtccaggtg tggcgggtga gtccgcgagc gcgctacgcc ccgccaaaga
      721 acctcaagta gcgccacgtc atgtcgcgga cacccgggat gcgtatgacg cgctcccgcg
      781 cgaactcgag ttccttcgcc atgtcgatgg ccgcgttctt gttggcgggt ttgatgattc
      841 ccccgagctc aacgtgcccg tgggcgacgt ggatcatgag ctgcgacgca tcgagggatc
      901 ggcggacaat ctcgcggcgc acgcgcatgg caatctgctt atcctcaaca gccatcgatc
      961 gtccctccat cggcggtgcc cgcccgcttg cgagcgggca ccgcgcgcca tccagcctcc
     1021 ggattcgcta cggggcggcc ggctcctccg gggcgcccac ggtcttcagc agctcggcca
     1081 ccgcggcctc gatctgggga tcgcggccat cgcgataggc ctcatacggg tagggcacct
     1141 cgatgtcggg cggcacaccg tagttctcca ggttggtgcc gttcagggta taccagcccg
     1201 tcacgggaat ccggaacccg tggccattca ggagggtgat atcataagtg ccaatcacac
     1261 ccccataggt tcggaccccg acgatcttgc ccagcttctt ctctcggaag ccattgggga
     1321 agatctccga gtcgctggca cagtgctcat tgatgataag cactttcggc ttgtcgtact
     1381 ggcggatggg ctgagtaacc gccggcgcgt tccgctggtt ctcgtagaca tggaccttct
     1441 tggtgagctc ttcgagcagg tcatcgtgga tgcggccccc gccgttgtag cggacatcga
     1501 tgaccagccc ctcgtagcgc tgggcctcgc cgaacagatc gcggcggaag cgctccagcg
     1561 atgccccgtc catgctccgg atgtgcaggt agtacagcct tccacccgaa agctcgagca
     1621 ccctgttgcg gttgtcccgg acccactgtt cgtaccgcag gttcgcccac tcgccaccgc
     1681 cgatgggctt cacggagatc gtccgggcgc cctcggcctc gggcttggag ttcacggtga
     1741 gcttgactcg cttgccgacc atccccttga gggcatcgaa gagactctcg ccaaactgca
     1801 catccttccc gttgatctgc aggatgtact cgccggccgc cacctcgctg cccgctttgg
     1861 tgcacgggcc gttgtagaga cgaccgatca ccttcacgcc tggaccctcg tacgactcat
     1921 cgtagtcgag ccccagcagg ccggtttccg ggccactgat gccgcccccg
//
LOCUS       DAOSPL010000978         1969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_57793_length_1969_cov_3.059039, whole genome shotgun sequence.
ACCESSION   DAOSPL010000978 DAOSPL010000000
VERSION     DAOSPL010000978.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57793_length_1969_cov_3.059039"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PLQ54_15910"
     CDS             <1..724
                     /locus_tag="PLQ54_15910"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOS94805.1"
                     /translation="YLRHLSPWSEVGVWTPSLLRNLLAANVLMAVFNLLPAFPMDGGR
                     VLRAMLAQRMPYARATRLAATIGQGLAVLLGLFGLFGNVLLLYVALFVYMGAQSEAAA
                     VQLQEDLKGLTVGDVAIPLTESITPGESLGAVLERFRTGFWGELPVVEEGDLRGVLTH
                     PLLLAAALEYPADTPVARLLGPVCPAVSAQDPLESLVMQFRSGQCWALAVMRDGRLAG
                     IVTAEGVGRFLMARSSLRSEDR"
     gene            complement(754..>1969)
                     /locus_tag="PLQ54_15915"
     CDS             complement(754..>1969)
                     /locus_tag="PLQ54_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94806.1"
                     /translation="CLTVERDKVREAFGQADLLQVELYRRDFRGYEGMENALRRFADA
                     GLGDRAIPILAAGPGNALWITTERELREQVAFVRWAVPDAPGVGFFPGFYPGLAEAAD
                     RAIYDYFLRPALLVRDGWLRNIGQLTATRVRVTLPDGTEKEMGTLFSGQRVAVGDGAR
                     CVPGADYTVVEWAPPTSRPSPDAAATAEQRAFEAAVLGAAPKMAIAPDALSLTRATSD
                     EADVNGQISGATVALGRALSGQPIGLSVSMMLRRTGYYGIAGCWLQGAGGGRLGFSIS
                     RGDHDRDLPGTNPRAEFAVWSDAKTPVADTCPLGLEPGVAYRLFVGHDGASTARMALW
                     GPGDALLWTSGPIPLAGPIRVDTLQFEVKPFDGSDVRLEPEGLLLRGVSGGEKPSPYV
                     IEALLSGITVSQ"
BASE COUNT          276 a          694 c          698 g          301 t
ORIGIN      
        1 ctacctgagg cacctgagtc cctggagcga agtgggggta tggacgccca gcctgctgcg
       61 gaacctgctg gcggcgaacg tgctgatggc tgtgttcaac ctcttgcccg ccttcccgat
      121 ggatgggggg cgggtgctgc gggccatgct ggctcagcgc atgccctatg cccgcgcgac
      181 tcgtctggcc gccactatcg gccagggcct cgcggtcctg ctggggctgt ttggcctctt
      241 cggcaacgtc ctgctgctgt acgtggcgct cttcgtctac atgggggcgc agagcgaggc
      301 cgccgcagtt cagttgcagg aggacctgaa ggggctcacg gtgggtgatg tcgccatacc
      361 cctgaccgag agcattaccc ccggggagtc tctcggcgcc gttctcgagc gtttccgcac
      421 cggcttctgg ggcgagctcc cggtggtcga ggagggcgac ctgcgcggcg tcctcaccca
      481 tcctctactg ctggcggcgg ccctcgagta cccggccgac acgccggtcg ccaggcttct
      541 gggacccgtc tgcccggccg tcagcgctca ggacccactc gagagcctcg tgatgcagtt
      601 ccgatccggc cagtgctggg cgctggcggt catgcgtgac ggccggctcg ccggcatcgt
      661 cactgccgag ggagtggggc ggttcctgat ggcccgatcg tcactccggt ccgaagaccg
      721 ctgacggcct gccggcccgg cgtcctccgc tggttactgg ctcaccgtga tgcccgacag
      781 cagcgcctcg atgacgtagg gcgagggctt ctcgccgccc gagacgccgc ggaggaggag
      841 gccttcgggc tccaggcgga catcactgcc gtcgaagggt ttcacctcga actggagcgt
      901 gtcgacgcgg atcggccccg cgagcgggat cgggccgctg gtccacagca gggcatcgcc
      961 gggtccccag agagccatcc gggccgttga ggcgccgtcg tggccgacga acagccggta
     1021 ggccacgccg ggctccagcc cgagcgggca cgtgtcggcc acgggtgtct tggcgtcgga
     1081 ccagaccgcg aactcggcgc gcgggttcgt gcctggcagg tcgcggtcat ggtcgcctcg
     1141 cgagatcgag aacccaagac ggcccccgcc ggcgccctgc agccaacagc cggcgatccc
     1201 gtagtagccg gtgcgtctca gcatcatcga caccgacagg ccgatgggct gccctgacag
     1261 cgcgcggccg agcgccacgg tggccccgga gatctggccg ttcacatcgg cctcgtcact
     1321 ggtggctcgg gtcagagaga gcgcatcagg cgcgatggcc atcttcgggg ctgccccaag
     1381 cactgcggcc tcgaaggccc gctgctccgc ggtggcggcc gcgtcgggac tcgggcgcga
     1441 ggtcggcggg gcccactcga cgaccgtgta gtccgcgccc gggacgcagc gcgcgccgtc
     1501 gcctacggcc actcgctggc cagagaacag cgtgcccatc tccttctcgg tcccatcggg
     1561 caaggtcacg cgcacgcggg tcgcggtgag ctgcccgata ttgcgcagcc agccgtcgcg
     1621 cacgagcagc gccggacgga ggaagtagtc gtagatcgcc cgatcggccg cctcggccag
     1681 gccggggtag aagccgggga agaacccgac gcccggcgca tcgggcacgg cccagcgcac
     1741 gaaggccacc tgctcccgca gctcgcgctc ggtggtgatc cacagcgcgt tgccgggccc
     1801 ggcggctagg atcgggatcg cccgatcgcc gagcccggca tcggcgaagc gccgcagtgc
     1861 gttctccata ccttcatagc cacggaagtc gcgccggtag agctcgacct gcagcagatc
     1921 cgcctggccg aacgcctctc ggaccttgtc ccgctcgacg gtaaggcag
//
LOCUS       DAOSPL010000979         1969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_57799_length_1969_cov_2.591954, whole genome shotgun sequence.
ACCESSION   DAOSPL010000979 DAOSPL010000000
VERSION     DAOSPL010000979.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57799_length_1969_cov_2.591954"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="PLQ54_15920"
     CDS             <1..864
                     /locus_tag="PLQ54_15920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS94807.1"
                     /translation="SKKAVLRRELEPGVAIVEGDATQLRQIVMNLITNASEALDDDPG
                     VITIAASVIEADREYLLTTYIDDDLQPGRYVALEVSDTGHGMDEDTKSRMFDPFFSTK
                     RTGRGLGMAAVLGIVRGHGGAIKVYSERGRGTCVKVLLPCVEPSVPSAMNGTSDTSAP
                     GSEVLSGTILVADDEEAVRQVTRLALEMAGFTVITAADGEEAVECFAAHASEVRAILL
                     DLSMPKMSGDEAFAAIKRIRPDVPVILSSGFNEQDTTNRLAGRGLAGFIQKPYRPTEL
                     IEKLRQLLRGA"
     gene            complement(897..>1969)
                     /locus_tag="PLQ54_15925"
     CDS             complement(897..>1969)
                     /locus_tag="PLQ54_15925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOS94808.1"
                     /translation="VRETMGRVTAPVLLTGGWYDYYPGETFRNYQALVESAPSPEAAA
                     SHRVIVGPWTHGINAQTQLGQIDFGDAALLENDSTQRWLECLLGGGSPTDFQHAPIRI
                     FVMGTNRWRNEYEWPLARTKYTRLYLHSSGRAGSSLADGKLSPVAPGTEPPDCYRYDP
                     DRPVPTLGGNHSVGPYNPGLYELALPGPYDQRTNERRDDVLVYTTDELDEDTEVTGPV
                     TVTLFASTSARDTDFVARLADVYPDGRSIHITEGVIRARYRKRDWAHPELVEPNRVLE
                     YTIELQPTSNVFRKGHHIRLQVTSSNFPLWDRNLNTGNPPGTDTEPRVAEQTVCHTGQ
                     YPSHVTIPVIPPGETEAVLAEP"
BASE COUNT          325 a          669 c          631 g          344 t
ORIGIN      
        1 tccaagaagg ccgtgctccg gcgcgaactg gagcccggcg ttgccatcgt cgagggcgac
       61 gcgacccagc tccggcagat cgtgatgaac ctcatcacga atgcatccga ggccctcgac
      121 gacgatcccg gcgtcatcac catcgccgcc tccgtgatcg aggcggatcg cgagtacctc
      181 ctgaccacct acatcgacga cgatctgcag cctgggcgat acgtggccct cgaggtgtca
      241 gacaccggtc acggcatgga tgaagacacc aagtctcgca tgttcgaccc gtttttcagc
      301 accaagcgca ccggccgggg cctcggcatg gctgccgttc tcggcatcgt tcgtggccac
      361 gggggcgcca tcaaagtcta cagcgaacgc ggccggggaa cgtgtgtcaa ggtcctcctg
      421 ccttgcgttg agccaagcgt cccatcggcc atgaacggca cgtctgacac ctcagctccc
      481 ggctcagagg tgctctcggg gaccattctg gtcgccgatg atgaggaggc cgtccggcag
      541 gtgacccgcc tcgccctcga gatggccggc ttcaccgtca tcacagccgc cgacggcgag
      601 gaggccgtcg agtgctttgc cgcccacgcc tccgaagtgc gggccatcct gctggacctg
      661 agcatgccga agatgagcgg tgatgaggcg tttgctgcga tcaagcggat ccgtcccgac
      721 gtcccggtca tcctctccag tggcttcaac gagcaggata ccaccaaccg cctcgcgggc
      781 cggggcctcg ccgggttcat ccagaaaccc tatcggccca ccgaactcat cgagaagctc
      841 cgccaactgc tccgaggcgc ctgagcgcgg tccgcgcacc ggctcgatca ctggccctaa
      901 ggctcagcga gcacggcctc cgtctccccc ggcgggatca ccgggatcgt cacgtgcgag
      961 gggtactgac ccgtgtggca gaccgtctgc tcggccacgc gtggctcggt gtccgtcccc
     1021 ggcgggttcc ccgtgttgag gttgcggtcc cacaaaggaa agttgctact cgtaacctga
     1081 agccgaatgt ggtggccctt acggaacacg ttgctcgtgg gctggagctc aatggtgtac
     1141 tcgagcaccc ggttgggctc caccagctcg ggatgggccc agtcgcgctt gcggtaccgg
     1201 gcacggataa caccctcggt gatgtggatg gacctcccat cggggtacac gtcagccagc
     1261 cgagccacga agtccgtgtc gcgcgccgat gtggaagcga agagggtcac cgtcaccggg
     1321 cccgtgacct ccgtatcctc gtccagctcg tcagttgtat agaccagtac atcgtcgcgc
     1381 cgctcgttcg tcctctggtc gtacggtccc gggagcgcca gctcgtacag gcccgggttg
     1441 tacggtccca ccgaatggtt tccccccagg gtcggcaccg ggcggtccgg gtcataacgg
     1501 tagcagtcgg gcggttccgt ccctggcgct acaggggaga gcttcccgtc ggccagcgat
     1561 gagccagctc ggccggacga gtgcaggtag agcctcgtgt acttggtgcg cgccagcggc
     1621 cactcatact cgttccgcca ccgattggtc cccatgacga agatccggat gggcgcgtgc
     1681 tggaagtcgg tgggactccc gcccccgagc agacactcga gccaccgctg cgtgctgtcg
     1741 ttctcgagaa gggcggcgtc accgaagtcg atctggccca gctgtgtctg tgcgttgatg
     1801 ccgtgcgtcc agggcccgac gatgacgcgg tggctcgccg cagcctccgg gctcggagcg
     1861 gactcgacga gcgcctggta gttgcggaat gtctcaccag gatagtagtc ataccacccg
     1921 cccgtcagca gcacgggtgc cgtgacccgc cccatggtct ctcggacgc
//
LOCUS       DAOSPL010000980         1969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_57812_length_1969_cov_2.055904, whole genome shotgun sequence.
ACCESSION   DAOSPL010000980 DAOSPL010000000
VERSION     DAOSPL010000980.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57812_length_1969_cov_2.055904"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..1377
                     /locus_tag="PLQ54_15930"
     CDS             268..1377
                     /locus_tag="PLQ54_15930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS94809.1"
                     /translation="MKVLYASFDVVPAPKGATTHIARFVEALVGQYGSVDLMTLGPSP
                     SRPESRFYGARHLRVCPPSTHYLDQASCFRSAVDEQLRAEPYDLVHFRCIWAGLPAIA
                     AAPRLGFRTVYEANGFPSIELPYHFPGLARRPDLLARLSEQEAECLQRADAVVVPSDV
                     TRAYAISRGAPEGHVRTIRNGVDPELFRPVHRDIEPGGRVRLLYVGTLAPWQGVGLLV
                     DAFARACRHADLELVLLAPNKGQWARPLLKRIAKLGVAERVRLLPPRSLAGVPAAVAA
                     ADICLAPLANTRRNRVQGCCPIKVPEYLACERAVVAAGLPVVREVLTHGETAWLYRAG
                     DARSLRNAIVTLAGDPALRARLGAAGRDMVCRRLT"
     gene            complement(1407..>1969)
                     /locus_tag="PLQ54_15935"
     CDS             complement(1407..>1969)
                     /locus_tag="PLQ54_15935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS94810.1"
                     /translation="ELRFKKGLHIALDAFRRLSEQRPAQLLLVGGARKPERQLLRNFL
                     EAHPSLRPHVRLVEAIVDPEEMVRHYSLLDVLLCPSLWEGMPNAVLEAMACGCCVVAS
                     DAGGLKDLIRHGETGALMVRDELSHLAELLAELLDAGPGARAEMGARARHFVTREHSP
                     EIEAERTVDLYRAALGPRVPSARSPS"
BASE COUNT          275 a          693 c          669 g          332 t
ORIGIN      
        1 cgacaggcct aggagcatga aggacacaac cacacccagc gcggccaacg cgatgcggat
       61 gccccacgtc cgcctggccc gctcggcttg ggcatcgcgc cgactccagg catcgatttg
      121 gtttcgcact tcctccgccg tgccgtgcgc tcggaactcc ttggccatac tcgggctctc
      181 cccacttgcg ccgtgtcgca caccgggcgt gggttcgcgc tggacgtggc gcctcacctg
      241 cgccggcacc tcgtacataa tggcggtatg aaggtgctct atgcctcgtt cgacgtggtg
      301 ccggcgccca agggggccac cacgcacatc gcccgcttcg tcgaggccct tgtgggccag
      361 tatggcagtg tcgacctcat gacgttgggg ccctcaccgt ctcggcccga gagtcgtttc
      421 tatggcgcac ggcaccttcg tgtctgtcca ccgagcactc actacctgga tcaggcctcg
      481 tgcttccggt cggccgtgga cgagcaactc cgcgccgagc catacgacct cgtgcacttc
      541 cgctgcatct gggcggggct gccggctatt gccgccgcac cccggctggg atttcgcacg
      601 gtgtacgagg ctaacggctt cccgagcatt gagctcccgt accacttccc cgggttggcg
      661 cgccgccccg atcttctggc gcggctatcc gagcaggagg ccgagtgtct ccagcgagcc
      721 gatgccgtgg tcgttcccag tgatgtgacc cgggcgtacg cgatctcccg cggcgctcct
      781 gaaggacacg tgcggaccat ccgcaacgga gtcgatcccg agctgttccg gccggtgcat
      841 cgtgacatcg agcccggcgg ccgcgtgcgg ctgctctatg tgggcacgct cgcgccctgg
      901 cagggggtgg gtctgctcgt ggacgcgttc gcgcgggcgt gccgccacgc agacctcgag
      961 ctggttctcc tcgcacccaa caagggccag tgggctcgcc cgctgctcaa acggatcgcg
     1021 aagctcgggg tcgcggagcg ggtgcggctc ctgccgccgc gttcgctggc cggcgtcccc
     1081 gctgccgttg cggcggcgga catctgcctg gccccgctgg cgaacacccg gaggaaccga
     1141 gtccagggct gctgccccat caaggtgccc gagtacctgg cgtgcgagcg ggccgtggtg
     1201 gcagccggcc tcccggtggt gcgcgaggtg ctcacgcatg gcgagacggc atggctctac
     1261 cgcgccggcg atgcgcgctc actccggaac gccatcgtga cgctggcggg cgatccagcc
     1321 cttcgggctc gcctgggtgc ggccggcaga gacatggtgt gccggcggct cacgtgacgc
     1381 gcggcccaga cgaggctgat cgagctctat gaggggctcc gggcgctcgg gacccggggt
     1441 cccagagcgg ctcggtagag gtccaccgtt cgttcggcct cgatctccgg gctgtgctcg
     1501 cgggtcacga agtgtcgggc gcgagcgccc atctcggcac gggcccctgg accagcatcg
     1561 agcagttccg ccagcagctc ggccaggtgg gatagctcgt cgcgcaccat cagggccccc
     1621 gtctcgccgt ggcggatcag gtccttgagg ccaccggcgt cgctcgccac cacgcagcag
     1681 ccacaggcca tggcctcgag cacggcgttg ggcataccct cccacagcga ggggcacagt
     1741 agcacgtcaa ggagactata gtgccttacc atttcctccg gatcgacgat ggcctccacc
     1801 aggcgcacgt gcggccggag actggggtgt gcttcgagga agttgcggag cagctgcctc
     1861 tcgggtttgc gtgcgcctcc aacgaggagc agctgcgccg ggcgttgctc cgacagccgc
     1921 cggaaggcat cgagggcaat gtggagcccc ttcttgaacc gcaactcgc
//
LOCUS       DAOSPL010000981         1968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_57831_length_1968_cov_3.741244, whole genome shotgun sequence.
ACCESSION   DAOSPL010000981 DAOSPL010000000
VERSION     DAOSPL010000981.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57831_length_1968_cov_3.741244"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLQ54_15940"
     CDS             <1..251
                     /locus_tag="PLQ54_15940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94811.1"
                     /translation="GNDTIAGGSGSDTANYTANPRPPYTPFEGGSASETHYPTRHNGG
                     LNFLFYDGHAKWLKPSALRVKYFREPGYGPAVPGYPGE"
     gene            334..>1968
                     /locus_tag="PLQ54_15945"
     CDS             334..>1968
                     /locus_tag="PLQ54_15945"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOS94812.1"
                     /translation="MRVRVVLAVHALLLLPMVRAGGQVPAMAPSEAASGAADGQRFAL
                     VIGVSNYDDARFKDLAYADSDARALAAVLTDPTRGRFAKENVRLLTSDGSGSPPTKNN
                     IFREMQWLRANSGPNDLILVFFAGHGFRDEFGTYLVPKDGEWDVLVSSGVNMNDFNRF
                     VNAMGARRKIVVFDCCHAGGISNTARGEEPLDAEYYREACAQAEGRINFSACRAGELS
                     YEYDEQKHGVFTYHLCQALAGHADRDLNGVVTFAEVESYVKREVVKWAQGQGKQQNPQ
                     VDAVALSGEFVLAENPDAAGSPRVAEAALKVRDLVHRGVLTSEEGTLLVEALRARTNP
                     AGSGSGVSTVAMSSAARAALLTLGDPVIDAAFQLELESPPAIVGDPPLDRTEGGFSFS
                     GSQPGPYHRPGDRWNQARDGVPMAYVRGEEREFGYRTSDIAALGPLGLVAADDEDLRA
                     HASEYGRRPVGSFVIDVTEVTIGQYAEFVAQTGHAAPEGWPPGGPTDDMKCLPVTGVS
                     WDDAMAYAKWAGKRLPSADEWEAAARGRECRTFPWGE"
BASE COUNT          314 a          692 c          649 g          313 t
ORIGIN      
        1 cggggaacga caccatcgcc ggcggaagcg gctccgacac cgccaactat accgcgaatc
       61 cgcggccgcc ctacacacca ttcgagggcg gcagcgcctc cgagacgcac taccccaccc
      121 gccacaacgg cggcctgaac ttcctcttct atgacggcca cgccaaatgg ctcaagccct
      181 cagcgcttcg cgtgaagtac ttccgcgagc ccgggtacgg ccccgccgtg cccgggtacc
      241 ccggcgaata gcatccccct ccccggctga cgtgcggccc cccggaactc accggggggt
      301 tcgcgcgtcc aaacggtggc tgggaggatg aacatgagag tcagagtcgt gctcgcggtc
      361 cacgcgctgc tgttgcttcc catggtgcgc gccggcgggc aggtgcccgc catggctccg
      421 agtgaggcgg cctccggcgc tgcggatggc cagcgcttcg cgctcgtgat cggcgtgtcc
      481 aactatgacg atgcccggtt caaagacctc gcctatgccg actccgacgc ccgcgccctc
      541 gccgccgtgc tcaccgaccc cactcgcggc cggttcgcca aggagaacgt gcggctgctc
      601 accagcgatg gttccggcag cccccccacg aagaacaaca tctttcggga gatgcagtgg
      661 cttcgggcca actccggccc caatgatctc attctggtct tcttcgccgg ccacgggttc
      721 cgtgacgagt tcggcacgta tctggtcccc aaagacggcg agtgggacgt gctggtctcc
      781 agcggcgtca acatgaacga cttcaaccgc ttcgtgaacg ccatgggcgc ccgccggaag
      841 attgtggtct tcgactgctg tcacgccggc gggatctcga acacagcccg cggcgaagag
      901 cccctcgatg cggagtacta ccgcgaggct tgtgcccagg ccgagggccg catcaacttc
      961 tcggcgtgcc gtgccggcga gctctcatac gagtacgacg agcagaagca cggagtcttc
     1021 acataccacc tgtgtcaggc cttggcgggc catgccgacc gtgacctcaa tggcgtggtg
     1081 accttcgccg aggtcgagag ctatgtgaag cgcgaggtcg tgaagtgggc tcaaggccag
     1141 gggaagcagc agaaccctca agtcgacgcg gtggcgctgt ctggcgagtt cgtcctggcc
     1201 gagaatccgg atgctgccgg ctcacctcgc gtcgccgagg cggcgctcaa ggtgcgcgac
     1261 ctggtccacc ggggtgtgct cacgagtgag gagggcaccc tgcttgtcga ggcgctccgc
     1321 gcccggacca atcccgctgg atcgggctcc ggcgtctcca ccgtggccat gtcatcggcc
     1381 gcgcgcgccg cgctgctcac gctgggcgat cccgtcatcg atgccgcgtt ccagctggag
     1441 ctcgagtcgc cgcccgctat cgtgggcgac ccgcccctcg atcgaactga gggcgggttc
     1501 agcttcagcg gcagccagcc tggcccgtac caccgccccg gcgatcggtg gaaccaagct
     1561 cgcgatggtg tccccatggc ctacgtccgt ggcgaagagc gcgagtttgg ataccgcacc
     1621 tccgacatcg cagccctcgg gccgctgggg ctcgtggcgg ccgacgacga ggacctccgc
     1681 gcccacgcct ccgagtacgg ccgccggccc gtggggtcgt tcgtgatcga tgtcactgaa
     1741 gtgaccatcg gtcagtacgc cgagttcgtg gcccagaccg gtcatgccgc gcccgagggt
     1801 tggccgcccg gcgggcccac tgacgacatg aagtgcctcc ccgtcacggg ggtctcatgg
     1861 gacgatgcca tggcctatgc caagtgggcg gggaagcggc tgcccagtgc cgatgagtgg
     1921 gaggccgcgg cacgcggacg ggagtgccgc acgttcccct ggggcgaa
//
LOCUS       DAOSPL010000982         1967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_57909_length_1967_cov_2.034519, whole genome shotgun sequence.
ACCESSION   DAOSPL010000982 DAOSPL010000000
VERSION     DAOSPL010000982.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57909_length_1967_cov_2.034519"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1967)
                     /locus_tag="PLQ54_15950"
     CDS             complement(<1..>1967)
                     /locus_tag="PLQ54_15950"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOS94813.1"
                     /translation="IVFGPEDSGTAECPITYEAAPGAKPVFTGGVRITGFREGPDGVW
                     QTTVEDVARGGWYFEQLFINGRWATRARSPNQFYYYMIGKVARGIDPATGQLADLSAR
                     AFRARPGDVKPWPNLNDVVLVAYHSWETSQHRLAAVDPETNTVICTGPAAWAFMQWGF
                     MQRYHVENFREALDQPGEWYLDRDGTLSYIPLPGETPDYVEVIAPVTEQFVHFVGETA
                     LGIPVEHITLRGLAFRHAAYTLPPEGHSDGQAAASMPAVIMADGAQSITIDQCEVGYV
                     GVYGIWFRRGCRDCAVQRSYIHDTGAGFVRIGEGIIQPDEADRTSHIVVDNNVMHIGG
                     RVDAGCVGVWIGQSGNNQVTHNDISDLFYTGISAGWTWGYGPSLASNNTIDFNHIHHI
                     GWGVLSDMGGVYTLGISPGTTVSNNVIHHVYSYDQYGRGGWGLYNDEGSSDMVLENNL
                     VYRVKTGTYHQHYGQANVIRNNILCYSMDGQIQRSRVEDHLSFRYGRNLVCWNGGDLI
                     TAGSIRDANVQLDSNLYWNEAGPVTVHGMSFEDYQATGHDPNSLVADPMFVDPAHDDY
                     RLQPGSPAEKIGFRPFDYSRAGVYGDADWVALPKSFEYPEVEFAPPPPPPPPLVIHDG
                     FELSALGEPPADAQCFVEGRGDGLTVVDEGPA"
BASE COUNT          340 a          603 c          673 g          351 t
ORIGIN      
        1 gccggcccct catccaccac ggtcagaccg tcgcctctgc cctcgacgaa gcactgggca
       61 tcggcgggtg gctcgccgag ggctgacagc tcgaaaccgt cgtggatcac cagcgggggc
      121 ggcggaggtg gcggcggggc gaactcgacc tcggggtact cgaacgactt ggggagtgcc
      181 acccagtcgg catcaccgta aacgcccgct cggctgtagt cgaagggccg gaacccgatc
      241 ttctcggcgg gcgagcccgg ctgcagacgg tagtcatcgt gagcgggatc gacgaacatc
      301 gggtcggcca ccagcgagtt cgggtcgtgt cccgtagcct ggtagtcctc gaaggacatc
      361 ccgtggaccg tgacggggcc cgcttcgttc cagtacaggt tgctatcgag ctgcacattc
      421 gcgtctcgga tcgaccctgc ggtgatgaga tcaccgccgt tccagcaaac caggttgcgt
      481 ccatagcgga aggacagatg gtcttcgacg cgcgagcgct ggatctgccc gtccatgctg
      541 tagcagagaa tgttgttccg gatcacgttc gcctggccgt agtgctggtg gtaagtaccc
      601 gtcttgacgc ggtagaccag gttgttctcg agcaccatgt cagagctgcc ttcgtcgttg
      661 tacagacccc atccgccacg gccgtattgg tcataggagt agacatggtg aatcacattg
      721 ttactgaccg tcgtgccggg tgagatgccg agcgtgtaga ccccgcccat gtcggagagc
      781 acaccccaac cgatgtggtg gatgtggttg aagtcgatgg tgttgttcga ggccaagctg
      841 gggccgtagc cccaggtcca cccggccgag atgcccgtgt agaacaggtc ggagatgtcg
      901 ttgtgagtca cctggttgtt gccgctctga ccgatccaga cacccacaca cccggcgtcg
      961 acgcgcccgc cgatgtgcat gacgttgttg tcgaccacga tgtggctcgt gcgatcggcc
     1021 tcatcaggtt ggatgatgcc ctcgccgatg cgtacgaagc cggcgcctgt gtcgtggatg
     1081 taagagcgct ggacggcgca gtcgcggcag ccgcggcgga accagatacc gtagacaccg
     1141 acgtagccga cttcgcactg gtcgatggta atggactgcg cgccgtcggc catgatgacg
     1201 gctggcatcg aagcggcggc ctgaccatcg ctgtgcccct ctggcgggag cgtgtaggcg
     1261 gcgtggcgga acgcgaggcc gcgcagggtg atatgttcca caggaatgcc gagggccgtc
     1321 tcgcccacaa agtgcacgaa ctgctcggtc accggggcga tgacctcgac gtagtccggt
     1381 gtctcgcccg gcaacgggat gtaggacagc gtaccgtcac ggtcgaggta ccactcaccc
     1441 ggctgatcga gggcttcgcg gaagttctcg acatggtagc gctgcatgaa cccccactgc
     1501 atgaaagccc acgcggccgg gccggtgcag atgaccgtgt tcgtctcggg gtcgacggcg
     1561 gccaggcggt gctgcgacgt ctcccatgag tggtaggcga cgaggacgac atcgttgagg
     1621 ttcggccatg gcttcacatc gcccggccgc gccctgaaag cgcgggccga caggtcagcg
     1681 agttggccgg tcgcgggatc gatgccccga gcgaccttgc cgatcatgta gtagtagaac
     1741 tggttgggag agcgggcccg ggtggcccac cggccgttga tgaacagctg ctcgaagtac
     1801 cagccgccgc gggcgacgtc ttcgaccgtg gtctgccaca cgccatccgg cccttcacgg
     1861 aagccggtga tgcgcacacc acccgtgaac acgggtttgg cgccgggcgc ggcctcgtag
     1921 gtgatggggc actcggcggt gccgctgtcc tcggggccga acacgat
//
LOCUS       DAOSPL010000983         1965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_57955_length_1965_cov_4.304712, whole genome shotgun sequence.
ACCESSION   DAOSPL010000983 DAOSPL010000000
VERSION     DAOSPL010000983.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57955_length_1965_cov_4.304712"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PLQ54_15955"
     CDS             <1..502
                     /locus_tag="PLQ54_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94814.1"
                     /translation="LLAACSSTPEPMPSNPAPSSPISIPTGLTPAPTDVPEQHDDGSI
                     GTPMPGSTLTLDEASRDSAIERGQTVMSLFARRAVDATQWQADLAPYLTPQAAQAYQY
                     VDPANVPPTKITGPVTLTPASTPLVARVSVPTDAGVYLVIMARTDESPTWLADRIMPP
                     EGQGDS"
     gene            502..1947
                     /locus_tag="PLQ54_15960"
     CDS             502..1947
                     /locus_tag="PLQ54_15960"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOS94815.1"
                     /translation="MSSGGQSSGLRTVAITALVIPALILAFLFGIVLISDTTSQAACG
                     PTGTALTVDPASVPEGPIAGYDHEQLVNAAHIMLAAQKLGLSARDQQIGVSTAMGESS
                     LRVLDYGDDVGPDSRGLFQQRDNGAWGSYSDRMDPFISATNFFRVEMTIAGRESMEPT
                     LVSNAVQRNADPYHYARFWEPAGAVVTALGGVQQAAAPAANAGAGGSEYSLGPVHPQT
                     AIVANTVGPMFGFVTIGGYRESARDPNGHPSGLALDFMTNDIPNGRTAGDNLAKYLQD
                     HAADLGVDYIIWLQRIWSTDRADEGWRPMEDRGSPTQNHMDHVHLNLTGKGSSAIPGC
                     ETGLPGEVSLAGWALPAQGPLNSDFGPRSSPGGIGSTYHRGIDLGGGGCYGPIWAANT
                     GQVVRAGPAGGYGNLIEIDHGSGVHTRYGHMFNDGVLAKVGDQVTAGQQIAKVGTTGT
                     STGCHLHFEVLVDGQQVDPQAFLAQVGVTIK"
BASE COUNT          349 a          742 c          607 g          267 t
ORIGIN      
        1 tcttcttgcc gcgtgcagca gcacgcccga gcccatgccg agcaacccgg ccccgagtag
       61 cccgatctcc attccgaccg gcctcacgcc ggccccgaca gacgtgcccg agcagcacga
      121 cgacggatca atcggcacac cgatgccggg ctccacactc accctcgacg aagcgtcgcg
      181 agacagcgcg atcgagcgcg gccagacggt gatgagcctg ttcgcccggc gcgcggtcga
      241 cgccacccag tggcaggccg acctcgcgcc gtacctgacg ccgcaggccg cccaggccta
      301 ccagtacgtc gacccggcca atgtgccgcc aaccaagatc accggcccgg tgacgctcac
      361 cccagcctcg acaccgctgg tcgcccgagt gagcgtcccc accgacgccg gggtctatct
      421 ggtgatcatg gcccgcaccg acgaaagccc aacgtggctc gcggaccgga tcatgccgcc
      481 cgagggtcaa ggagactcct gatgagcagc ggcggccagt cctcggggct gcgcacggtc
      541 gcgattactg ccctggtcat ccccgcgctc atcctcgcct tcctgtttgg catcgtgctc
      601 atctccgaca cgacctcaca agcggcctgc ggccccaccg gaaccgcgct gacggttgac
      661 ccggcgagcg tgcctgaagg cccgatcgcc ggctatgacc acgagcagct cgtcaacgcc
      721 gcgcacatca tgctcgccgc gcagaagctc ggcctcagcg cccgagatca gcagatcggc
      781 gtctcgaccg cgatgggcga gtccagcctg cgcgtcctgg actacggcga cgacgtaggc
      841 ccggacagcc gcgggctgtt ccagcagcgc gacaacggcg cgtggggctc gtactccgac
      901 cggatggacc cgttcatctc ggccaccaac ttcttccggg tcgagatgac cattgctggc
      961 cgagagtcga tggaacccac gctcgtgtcc aacgcggtgc agcgcaacgc tgacccgtac
     1021 cactacgccc ggttctggga gcccgccggt gccgtcgtca ccgcccttgg cggggttcag
     1081 caggccgcag ccccggccgc caacgcggga gcgggcggat cggagtacag cctcgggccc
     1141 gtgcaccccc agaccgcgat cgtcgccaac acggttggac ccatgttcgg cttcgtcacc
     1201 atcgggggct accgcgaatc cgcccgcgac cccaacgggc accccagcgg cctggcgctg
     1261 gacttcatga cgaacgacat tcccaatggc agaacggccg gtgacaacct cgccaagtac
     1321 ctccaggacc acgcggccga cctcggggtg gactacatca tctggctaca gcgcatctgg
     1381 tccaccgacc gcgccgacga gggatggcgt ccgatggagg accgtggcag ccccacgcag
     1441 aaccacatgg accacgtcca cctcaacctc accggcaagg ggtccagcgc catccccgga
     1501 tgcgagaccg ggcttcccgg agaggtgtcc ctggccgggt gggcactccc ggcgcaaggg
     1561 ccgctgaact ccgacttcgg accgcgcagt tctcccggcg gtatcggctc gacctaccac
     1621 cgaggcatcg acctcggcgg tggcggctgc tacggcccca tctgggccgc gaacacgggc
     1681 caggtcgtgc gggcaggacc cgccggcggc tacggcaacc tcatagagat tgaccacggc
     1741 agcggtgtcc acacccgtta cggccacatg ttcaacgacg gcgtgctcgc caaggtgggc
     1801 gatcaagtca ccgccgggca gcagatcgcc aaggtcggca ccaccggcac ctccaccggg
     1861 tgccacctgc acttcgaggt gctggtcgac ggccagcaag tagacccaca agccttcctc
     1921 gcccaggtcg gcgtcaccat caagtagtag ggagataccg atgca
//
LOCUS       DAOSPL010000984         1965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_57963_length_1965_cov_3.674869, whole genome shotgun sequence.
ACCESSION   DAOSPL010000984 DAOSPL010000000
VERSION     DAOSPL010000984.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57963_length_1965_cov_3.674869"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PLQ54_15965"
     CDS             complement(<1..326)
                     /locus_tag="PLQ54_15965"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOS94816.1"
                     /translation="MAVISATGLAVAAPIHDAAAADDTARLEELYRADPGSVNRGDNL
                     GATPLHHAAQGGCVRAVQWLLDHGADPNARRADGGTALHAAAAAGQVKAAEALVRAGA
                     AVAAKDG"
     gene            complement(518..1762)
                     /locus_tag="PLQ54_15970"
     CDS             complement(518..1762)
                     /locus_tag="PLQ54_15970"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS94817.1"
                     /translation="MRVPMAWLVGLAGLLAAGCAWAQGAGSYAPDEDGWVSLFNGEDL
                     SGWSLTDPNGPNGWSVVDGILTNTAPSTDLKIDRLLTDHELHVEFRVPKGGNSGVYLQ
                     GRYEIQVADSEGAEPESHMCGAIYGLIAPRTNACKPAGEWQTYDATFTAAKTGRDGNI
                     ISLPRVTVVFNGVTIIDDAEIPGVTGAALDDQMGLPGPLYLQGNHSSVEYRNIRYRPL
                     KSDWAPEEDFVPLFDGESLDGWEAITSGHGTGGKWTVEDGVITGTQDRPGNGGLLATT
                     DQFGDFEIRAQINPEWDIDSGLFLRCQPDGRCYQVTVDYRPGGQVGTLYGEGIGGWIQ
                     ENPDWVKYYKADDWNEIRAIITGQPPRTQVWLNGNLLIDKVDTEERLPAEGAIAVQVH
                     GGGDWQGRVTRFRNIRIRLIEP"
     gene            complement(1828..>1965)
                     /locus_tag="PLQ54_15975"
     CDS             complement(1828..>1965)
                     /locus_tag="PLQ54_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS94818.1"
                     /translation="DNAEIDGVTGSALDDKEGQPGPLYLQGDHTAVEYRHIRYRPLGP
                     E"
BASE COUNT          291 a          711 c          626 g          337 t
ORIGIN      
        1 ccatccttgg cggccacagc cgccccggcg cggacgagcg cctcggccgc cttcacctgg
       61 cccgcggcag ccgccgcgtg gagcgccgtg ccaccgtccg ccctgcgggc gttcgggtcc
      121 gcgccgtggt ccagtagcca ctgtaccgcg cggacacatc ccccctgagc cgcgtggtgt
      181 agcggcgtgg ctcccaggtt gtcgccccga ttcacgctgc ctggatcggc ccgatacagc
      241 tcctcgagcc gcgccgtgtc gtcggcggcc gccgcatcat gaatcggcgc cgcgaccgcc
      301 aggccggtgg cggagatgac cgccatgacc aacaccgcgg cgccagcgca acatggggtc
      361 tgtagtgcca tggattcgcg ctctgacctc cgccgagacc ggtgcagggg catggggtac
      421 aacgctccgc acccccgtca gcggcgcggc ccaactgcct ggggccgcca gtcgagggct
      481 ttgccccctc gatatccggc cattccgcgc cgtgccctca cggctcgatc aggcggatgc
      541 ggatgttccg gaaccgcgtc acgcgaccct gccagtcgcc cccgccgtgc acctggaccg
      601 caatggctcc ttcggcaggc agacgctcct ccgtgtccac cttgtcgatg agtaggttgc
      661 cgttgagcca gacctgagtg cgaggaggct gcccggtgat gatggcgcgg atctcgttcc
      721 agtcatcggc cttatagtac ttcacccagt ccgggttctc ctggatccag ccaccgatgc
      781 cctcgccata gagcgtgcca acctggccgc ccggccggta gtccaccgtg acctggtagc
      841 agcgcccatc gggctggcag cgcaggaaga ggccactgtc gatgtcccac tcggggttga
      901 tctgcgcgcg aatctcgaag tcgccgaact ggtccgtggt cgccagcagt ccaccgttgc
      961 ccggtctgtc ctgggttccc gtgatgacgc cgtcctccac cgtccacttg ccgcccgtgc
     1021 cgtgtcccga ggtgatggcc tcccacccat cgaggctctc accatcgaac agcgggacga
     1081 agtcctcttc gggtgcccag tcgctcttca gcggacggta ccggatgttc cggtactcaa
     1141 ccgacgagtg gttcccctgt aggtagagcg ggccgggcag ccccatctgg tcatcgagcg
     1201 cggcccccgt gacgccgggg atctcggcgt catcgatgat ggtgaccccg ttgaacacca
     1261 cggtcacgcg ggggagcgag atgatgttac cgtcccggcc ggtcttggcc gccgtgaacg
     1321 tcgcgtcgta cgtctgccac tcgcccgccg gcttgcaggc attggtgcgg ggcgcgatca
     1381 gaccgtaaat ggccccgcac atgtgcgact cgggctccgc cccttcgctg tcggcgacct
     1441 gaatctcgta gcggccctgg agatacacgc cgctattgcc acccttggga acacggaact
     1501 cgacgtgcaa ctcgtggtcc gtcaacagcc gatcgatctt gaggtctgtg ctgggcgcgg
     1561 tgttcgtcag gatcccgtcg accacactcc agccgttcgg accgttaggg tccgtcagtg
     1621 accagcccga caggtcctcg ccgttgaaca gagagaccca tccgtcctcg tcgggcgcgt
     1681 acgatcctgc gccctgagcc cacgcacagc cggcagcgag caggccggcc aacccgacga
     1741 gccaggccat tggaacacgc atgatgtgcc tcctctagac acgtgccatg gcgccccgtc
     1801 ccatgggcgg ccgcgccggc agaaagccta ctcaggcccc agggggcggt accggatgtg
     1861 acggtactcg acggcggtgt gatcgccctg cagatagagc gggccgggct gtccctcctt
     1921 gtcgtcgagc gccgacccgg tgacgccatc gatctcggca ttgtc
//
LOCUS       DAOSPL010000985         1965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_57966_length_1965_cov_3.320942, whole genome shotgun sequence.
ACCESSION   DAOSPL010000985 DAOSPL010000000
VERSION     DAOSPL010000985.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57966_length_1965_cov_3.320942"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..681
                     /locus_tag="PLQ54_15980"
                     /pseudo
     CDS             38..681
                     /locus_tag="PLQ54_15980"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(824..1612)
                     /locus_tag="PLQ54_15985"
     CDS             complement(824..1612)
                     /locus_tag="PLQ54_15985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS94819.1"
                     /translation="MARASIPPAIVVRDLRKRFVVSRDRPESLKSAAVQLFRRRTHHS
                     EAFWALDGVSFEVERGEVLGLIGPNGSGKSTLLGLLSRILRPTGGTVEVCGRVCTLLE
                     LGAGFHPELTGIENIYLNGVILGLSRSEVSERIESIVAFAELGDFIESPVRTYSSGMV
                     TRLGFAVAVHVEPDILLIDEVLAVGDERFTRKCYAKMNEFRADPRRTLILVSHDLEAV
                     GALCTRVMWLERGRIVELGEASDVLEHYLAKHGDGGGEHQAGAQ"
     gene            complement(1614..>1965)
                     /locus_tag="PLQ54_15990"
     CDS             complement(1614..>1965)
                     /locus_tag="PLQ54_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94820.1"
                     /translation="AALALGLSFLFSVANTLLRDVEYLLSFAFTLGFFVTPILYPAGV
                     VKQNLAEPYFTIYMLNPVAVAIEQTRFAILGAGDFPPPTSAQLAAGAATCVLALAAGY
                     AVFRRLQWRLPELV"
BASE COUNT          349 a          679 c          649 g          288 t
ORIGIN      
        1 cattctcgaa cccatccagg gcgagggggg cgtcatcgtg ccccccgatg gctacctggc
       61 cgccgcccgg gcggcctgcg accgcgctgg cgccctgcta atcctcgatg ggtgcagacc
      121 gggttcggtc gcaccggccg catgttcgcg tgcgaacatg agggcgtctg ccccgacgtc
      181 atgacgttgg ggaaagcctt gggcggaggc gtcatgccca tcggggcctt caccgctcgg
      241 ccacacctct ggaccatgtt cgccgagaac ccgcttctgc actcctccac gttcggaggc
      301 aacccgctgg cctgtgtggc cgggatcgcg gccattgaca ccaccgtcga acaggggctg
      361 cccgagcgag ccgcgaccct cggcgcccac gccaaagcct cgctccagcg cgtggcggac
      421 gcgcaccccg acgtcatcgc ggccgtgcgc ggccgtggcc tgatgctggg cgtgagcttc
      481 accgaccccg acatcggagg cctggtcatc gccggcctgg cccagcggcg cgtcctggca
      541 gcctacacgc tcaacaaccc gagtgtggtg cgcttccagc cgcccctcgt gatcgaggag
      601 gagctactcg acgaagtgat ccgccgcctc gaggactcag tggtccagac caaagagatg
      661 ctcgccggcc tcgaagagta ggagcgatcg gcaagccgac aggctccttc tgcccgccgc
      721 tcccgcagct gcctgtggct gttccttcgg gtctcgtgcg ggctggcggt tcagacccgc
      781 caggggcggt gtgtgtgtgg tagcccgggg cgggagctcc gggctactgc gcgccagcct
      841 ggtgctcgcc gccaccatcc ccgtgcttgg caagatagtg ctccaacacg tcgctcgcct
      901 cacccagctc gacgatgcgg ccccgctcga gccacatgac acgggtgcac agcgccccca
      961 cggcctcaag gtcgtgggag acaaggatga gagtccggcg aggatcggcg cggaactcgt
     1021 tcatcttcgc atagcacttg cgcgtgaagc gctcgtcgcc cacggcaaga acttcgtcga
     1081 tgagcaggat atcgggctca acatgcacgg ccacggcgaa tccgaggcgg gtcaccatgc
     1141 ccgacgagta cgtgcgaacc ggcgactcga tgaagtcgcc cagctcagcg aaggccacga
     1201 tgctctcgat ccgttcggac acctcggacc gggacagccc cagaatgacg ccgttaagat
     1261 agatgttctc gatgcccgtc agctcggggt ggaaacccgc gcccagttcg aggagtgtgc
     1321 agacgcggcc acagacctcc acggtgccgc ccgtgggtcg caggatacgc gaaagcagcc
     1381 cgagcagcgt actcttcccc gatccattgg ggccaatgag gcccagtacc tcgcctcgct
     1441 cgacctcaaa gctcacgccg tcgagggccc agaatgcctc ggagtggtgg gtgcggcgac
     1501 ggaaaagctg caccgcagcg gacttgagcg actcggggcg gtcccgggac acgacgaatc
     1561 gcttccgcag gtcccgaacg acgatggccg gtgggatcga tgcccgcgcc acgtcacacc
     1621 agctccggca agcgccactg gaggcgccgg aacacagcgt acccggccgc cagagcgaga
     1681 acacaggtgg cggcccccgc cgccaactgc gcgctggtcg gcggcgggaa gtcgcccgcg
     1741 cccaggatgg cgaaccgtgt ctgttcgatc gcgaccgcga ccgggttgag catgtagatg
     1801 gtgaagtagg gctccgccag gttctgcttc accacaccgg ccggataaag aataggggtc
     1861 acgaagaaac cgagcgtgaa cgcgaagctc agcaggtact ccacgtcacg cagaagcgtg
     1921 ttggccacgg agaacaggaa gctcaggccc agcgccagcg cggcg
//
LOCUS       DAOSPL010000986         1965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_57975_length_1965_cov_2.847644, whole genome shotgun sequence.
ACCESSION   DAOSPL010000986 DAOSPL010000000
VERSION     DAOSPL010000986.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57975_length_1965_cov_2.847644"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1457
                     /locus_tag="PLQ54_15995"
     CDS             <1..1457
                     /locus_tag="PLQ54_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94821.1"
                     /translation="VMALDGYRFFHHHAFAWGASSVVAEIGENIPCTNLQIAFTRGAG
                     RESGKPWGIDLSSWFAGTVTDYSYVPEVLEADAAMFSGHSPMLHKRFCYATWLAGANM
                     LWFENYRLLVMRESALRQLAGFETSGQPEKWRLSPVGEMAERLFRLDREKDRGTPYTP
                     VAVILDFAHGWSPRGCAPHRIWGRLPLSDGDRMMDHFFNLVYPWHEPGTGWEPEDAGL
                     GLEKLERGYLTETPYGDIFDGLTTATCHTLGNYPVAILVGEVRIDGALAQRLDDYVRA
                     GGTLVANARQVGSSLPTSLTGAALTGEVRRATEAICHLDGSTMRSAEFDYAVAEMHGA
                     RPVVTVPGSNDVLVSDHRVGAGHVVLTTPHYLLDSNQEPLGLMDHLLRHVTSGLVPVR
                     VSPGVQWMVNRAADGWVVGLLNNRGAYKQPRGMPWIREEETAAVLVELDEPCVGADEW
                     VAGTALDVRTDAHSTRVRLSVPSGDVRIVHLRTR"
     gene            complement(1491..>1965)
                     /locus_tag="PLQ54_16000"
     CDS             complement(1491..>1965)
                     /locus_tag="PLQ54_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94822.1"
                     /translation="RDRDFAAAQALCGRQVRAAETDGKLSGLGMAGPMTPPPIPIELL
                     TPTANADQAKITFQLAAMMGTATAELPGPDGSRIRLVLKRPQGKWLLVGVTTTGTYLP
                     APGKGGEGSRSPKSPTAESGRPKGPGEAGPPVDATHTPEAAPSKAKGLPGAGPAT"
BASE COUNT          287 a          685 c          654 g          339 t
ORIGIN      
        1 ccgttatggc tctggacggc taccgcttct tccaccacca cgcgttcgca tggggtgcct
       61 cctcggtcgt ggcagagatc ggcgagaaca tcccctgcac gaacctccaa atcgccttca
      121 cgcgcggcgc gggaagggag tctggcaagc cgtggggcat cgatctctct tcatggttcg
      181 ccggcaccgt gacggactac tcctacgtcc ccgaggtcct cgaagccgat gcggccatgt
      241 tcagcgggca ctcgcccatg ctccacaagc gcttctgcta cgcgacctgg ctggcgggcg
      301 cgaacatgtt gtggttcgag aactaccggt tgctggtgat gcgcgagagc gccctccgcc
      361 aactggccgg cttcgagacg tccggtcagc cggagaagtg gcgcttgtcg cccgtgggcg
      421 agatggccga gcgcctgttc cgcctggacc gcgagaagga ccggggcaca ccctacaccc
      481 ctgtcgccgt gatcctcgac ttcgcgcatg ggtggtcgcc gcggggctgc gcgcctcacc
      541 gaatctgggg acgcctaccg ttgtccgacg gcgatcgcat gatggatcac ttcttcaacc
      601 tggtgtaccc ttggcacgaa cccggaactg gttgggagcc ggaggacgca gggctcggcc
      661 tggagaagct cgagcgaggg tacctcaccg agaccccgta cggggacatc ttcgacggct
      721 tgacgaccgc cacatgccac acactcggca actaccccgt ggcgatcctg gtcggcgagg
      781 tgcgcatcga cggcgcgttg gcccagcggc tcgacgacta cgtgcgtgcc ggaggcactc
      841 tggttgccaa cgcccggcag gtgggctcct ccctcccgac ctcgctgact ggggccgccc
      901 tgaccggtga ggtgcgtcgc gccactgagg ccatctgcca tctcgacggc tccaccatgc
      961 gctcggcgga gttcgattat gccgttgccg agatgcacgg cgcccgcccc gtggttaccg
     1021 tccccggctc gaacgatgtc ctggtgtccg atcaccgcgt gggagccggg cacgtggtgc
     1081 ttacgacccc gcactatctc ctggacagca accaggagcc actcggcctc atggaccacc
     1141 tgctccgcca cgtgaccagt ggacttgtcc ccgtcagggt cagccccggc gtgcagtgga
     1201 tggtgaaccg cgctgccgac ggctgggtcg ttggcctgct caacaaccgg ggggcctaca
     1261 aacagccccg tggaatgccg tggatccggg aagaggagac ggccgccgta ctcgtggagc
     1321 tcgacgagcc ctgtgtgggc gccgacgagt gggtcgcggg caccgcgctc gacgttcgga
     1381 ctgatgcgca ctcaacccgc gtgcgcctgt ccgtgccgtc cggcgacgtg agaatcgtgc
     1441 atctccgcac acggtgagaa gggcatcact cgcgaatgaa cccgcccggg ctacgtcgcg
     1501 ggtccggctc ccggcaggcc cttcgccttc gacggcgcgg cctctggcgt gtgcgtcgcg
     1561 tctaccggtg gcccagcctc gcctggtccc ttcggccggc cgctctcggc agtcgggctc
     1621 ttgggcgacc tgctcccctc gccccccttg cccggcgccg ggaggtaggt ccccgtggtc
     1681 gttacaccga ccagaagcca cttcccctgg ggacgcttga gcaccaagcg gatcctggag
     1741 ccgtcggggc ccgggagctc tgccgtggcc gtgcccatca tggccgcgag ctggaacgtg
     1801 atcttcgcct gatctgcgtt cgcagttggg gtgagcagct caatggggat cggcggtgga
     1861 gtcatgggac cggccatgcc caacccgctg agcttgccgt cggtctcagc ggctctgacc
     1921 tggcgcccgc agagggcctg cgcggctgcg aagtcgcgat cccga
//
LOCUS       DAOSPL010000987         1964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_58015_length_1964_cov_3.370351, whole genome shotgun sequence.
ACCESSION   DAOSPL010000987 DAOSPL010000000
VERSION     DAOSPL010000987.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58015_length_1964_cov_3.370351"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..>1964)
                     /locus_tag="PLQ54_16005"
     CDS             complement(276..>1964)
                     /locus_tag="PLQ54_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94823.1"
                     /translation="DVALFSAVPGNAAWGSDWARHCPPKRLEPGRFEAQLDLVPAAPE
                     VRGTEAAALLGGRSLAIDAWTDQPFHLWESADEPLPLTVETANVNESPLDVRRSWWAR
                     DLDGTIVAEGDETRRLADEERWRETLTVPAPERGIVFVEVRAEANGQEAFTRTTLAVL
                     PPHEFGPGEESIFGLAATFPVPSQTDVDRLMQRMGVRWLRGGDPRRTEALGINANWHA
                     GSSPDAMEDRPAEREEYLRKLIRECDERGNPYLEFANEWNMIGGIGKAEQAEVYVRDW
                     LLPLDRIRRETGSKVKLLSLGLSGPDPAFLARVKELGGWDALDGLAMHPGRGNYTADC
                     TEREAPGEYWTFLGAVQQARAAVAQLGEKPIWVTEAYACTFPNSWWHDTLRHAAENIV
                     LTYALAMEEGLCCVDWYQLVDATWYDIGGVNHKDPEYHYGLLYRDGSVKPSLLAYCTI
                     AEALDEARFVRRMTFDSPTTHGLWFRRPDGTVAILWDRTDGYRLSEKSDEYASPEPWI
                     DPWPTKVAVTLPAPGGSVRVLDCIGRETIVPSQDGQVTLTLDGAPRIVYGLREE"
BASE COUNT          268 a          727 c          657 g          312 t
ORIGIN      
        1 gggccgatgg ttcccgcaca ccgatgcgcg ggcctgcggc ggggcatacc cacatcgtgc
       61 cccagtgccg gccggcgacc tcgcccacca cgaccgcagc agggccgggt cgcgacggtc
      121 ccccctctct tcgccgcccg caccacgccc agtcccgctc ctgtcgcatt cgctcggccc
      181 atgtgtgcgt ggcaccgcct ctcggggttc cgcgcggagc gccggatggg gtccggggcc
      241 tgggccccgg ccgccggagg cgaagggtct tcgccctact cctcccgcag cccgtacacg
      301 atccgcggcg cgccatcgag cgtgagggtg acctggccat cctgtgaggg gacgatggtc
      361 tcccggccga tgcagtccag cacgcgcaca ctcccgcctg gggcggggag ggtgaccgcc
      421 accttggtcg gccatgggtc gatccatggc tcggggctcg cgtactcgtc ggacttctcc
      481 gagagcctat agccgtctgt ccggtcccac aggatcgcca cggtgccgtc ggggcggcgg
      541 aaccacagcc cgtgggtcgt cgggctgtcg aaggtcattc tgcggacgaa ccgcgcctcg
      601 tccagcgcct cagcgatcgt gcagtaggcc agcaggctcg gtttcacgct gccgtcgcgg
      661 tagagcaggc catagtggta ctcggggtcc ttgtggttca cgccgccgat gtcgtaccag
      721 gtcgcgtcga cgagctggta ccagtcgacg cagcagaggc cctcttccat cgccagtgcg
      781 taggtcagca cgatgttctc ggccgcgtgc cggagcgtat cgtgccacca gctgttcggg
      841 aaggtgcacg cataggcttc ggtgacccag atgggcttct ccccgagctg cgccacggcc
      901 gccctggcct gctggacggc gccgaggaag gtccagtact cgcccggcgc ctcgcgctcg
      961 gtgcagtcag ccgtgtagtt gccgcggccg ggatgcatag ccaggccgtc gagcgcgtcc
     1021 cagccgccga gctccttcac acgcgccagg aatgccgggt cggggcccga gaggcccaga
     1081 ctgagcaact tcaccttcga gccggtctcg cggcggatcc ggtccagcgg gagcagccag
     1141 tcacggacgt acacttccgc ctgttcggcc ttgccgatgc cgccgatcat gttccactcg
     1201 ttggcgaact cgaggtaggg gttgccgcgt tcgtcgcact cgcggatcag tttccggagg
     1261 tactcctcgc gttcagccgg cctgtcctcc atcgcgtcag gcgacgaccc cgcgtgccag
     1321 ttggcgttga tgccgagcgc ctccgtgcgc cgcggatccc ctccgcgcag ccaacgcacg
     1381 cccatgcgct gcataagccg gtccacatcc gtctgcgagg gcaccgggaa cgtcgccgcg
     1441 aggccgaaga tgctctcctc gcccgggccg aactcgtggg gtggcagcac cgcaagcgtc
     1501 gtccgcgtga aggcctcctg cccgttcgcc tcggcgcgga cctccacgaa cacgatcccg
     1561 cgctctggcg ccggcacggt cagcgtctcg cgccagcgct cctcgtcggc gagccgacgc
     1621 gtctcatcgc cctcggccac gatggtgcca tcgagatcgc gcgcccacca cgagcgcctc
     1681 acgtcgagcg ggctttcgtt gacgttcgcc gtttcgaccg tcagcggcaa cggctcgtcg
     1741 gccgactccc acaagtggaa tggctgatcg gtccaggcat cgatggcgag cgaccggccg
     1801 ccgagcagag cggccgcctc ggtgccccgc acttcgggcg ccgcgggcac caggtcaagc
     1861 tgggcctcga aacgcccggg ctcgagtcgt ttcggcgggc agtggcgggc ccaatcgctg
     1921 ccccacgcgg cattccccgg caccgcggag aacagcgcca catc
//
LOCUS       DAOSPL010000988         1964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_58019_length_1964_cov_3.182818, whole genome shotgun sequence.
ACCESSION   DAOSPL010000988 DAOSPL010000000
VERSION     DAOSPL010000988.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58019_length_1964_cov_3.182818"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PLQ54_16010"
     CDS             complement(<1..381)
                     /locus_tag="PLQ54_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS94824.1"
                     /translation="MARLSEEPDVVLQNQLERFAHQRGDGETPMAMVECQGLRKVYRD
                     LVAVNDLSLSIGEGEIFGFIGPNGAGKTTTIKMLVTLLRPDAGTALVDGKCVIREQEE
                     VRKVIGYMPDFFGVYDDIKVWEYLD"
     gene            complement(694..>1964)
                     /locus_tag="PLQ54_16015"
     CDS             complement(694..>1964)
                     /locus_tag="PLQ54_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HOS94825.1"
                     /translation="GMRDEAEIRGHRRTYVGALPGRIIQGLAQAGTRNPVFMLDEIDK
                     VGTDFRGDPSSALLEVLDPEQNNTFRDHYLDVDFDLSQVMFITTANVLDTIPRALLDR
                     MEVLRLPGYTHGEKLAIAERYLVRKQLAEHGLTRKQLRIPRATLSAVVTQYTREAGLR
                     NLEREIATLCRKRARSIAEGCEGAVTITPSSLPDYLGPPRFFDDIVERTTTPGVAAGL
                     AWTPTGGEVLFVECAVMPGKGNLTLTGHLGEVMRESAQAALTCVKRLAPELGIAVESF
                     DTKDLHVHVPAGQVPKDGPSAGTTIATAMASALTNRPVRHDLAMTGEITLQGKVLPVG
                     GIKEKALAAARARARTMVLPKRNEPDLLEVPDEIKARMEFVPVERIEQVLEHALLPAE
                     DPSDSRRPAGRPGRARKARPTPRGKRGAGA"
BASE COUNT          300 a          683 c          642 g          339 t
ORIGIN      
        1 gtccaggtac tcccagacct tgatgtcgtc gtagaccccg aagaagtccg gcatatagcc
       61 gatgaccttg cggacctcct cctgttcacg gatcacgcac ttgccgtcaa ccagggccgt
      121 gccggcgtcc ggccggagca gcgtgacgag catcttgatg gttgtggtct ttccagcgcc
      181 gttggggccg atgaagccga agatctcgcc ctcgccgatg ctcagcgaca ggtcgtttac
      241 cgcgaccaga tcgcggtaga ctttgcgcag gccctggcac tcaaccatcg ccatcggcgt
      301 ctccccatct ccgcgttgat gcgcgaatcg ttcgagctgg ttctgtagga cgacgtccgg
      361 ctcctcggag agtcgcgcca tcccggcact catacggatg ggcccgagtc actccgagtt
      421 ggacccggag cgaccagact acgcccgaag gggctcccgg cggaccaggc ctcacatggc
      481 ggcggccatt cgcgcccgga gggcgccgtc aggatcccca tcggcatctg ccacgacccg
      541 gagacgtcac cgcgaatgcg ctgggggcgg aatcccgcgc atgcggcaca cgcattcagc
      601 gctccgggcg cgccgagggc gagccgttcg ggggcgcacc cgatgccgtc gtgcccgacc
      661 cggacgtccg ggtgcgcgac agcgcccgcg tggtcacgct ccagccccgc gcttgccccg
      721 tggcgtcggg cgagccttgc gcgcccgccc cggccgcccg gccggtctgc ggctatccga
      781 tgggtcctcg gcgggcagca aagcgtgttc cagcacctgt tcgatccgct caaccggaac
      841 gaactccatc cgagccttga tctcgtccgg cacttcgagc aggtcgggct cgttgcgctt
      901 gggcagcacc atggtccggg ctcgggcccg ggctgccgcc agtgccttct ccttgatgcc
      961 gccaacaggg agcaccttgc cttggagggt gatctccccg gtcatcgcca aatcatgccg
     1021 gacgggccgg ttggtgagcg ccgaggccat ggcggtggcg atggtcgtac cggcggaggg
     1081 cccgtccttg gggacctgcc cggccggcac gtgcacgtgc aggtccttgg tgtcgaagct
     1141 ctcgacggca atgccgagct cgggggcgag gcgcttcacg catgtgagcg ctgcctgggc
     1201 cgattcgcgc atgacctcgc ccagatgacc cgtcagagtc aggttgccct tgcctggcat
     1261 gaccgcgcac tcgacgaaca gcacctcgcc gcccgtcggc gtccaggcca gtcccgctgc
     1321 cacgccgggg gtggtcgtgc gctcgacgat gtcatcgaag aaccgaggtg gccccaggta
     1381 atcgggcaga ctgctggggg tgatcgtgac ggcaccttcg catccctcgg cgatgcttcg
     1441 agcgcgcttc cggcacagcg tggcgatctc ccgttcgagg ttccggagtc ctgcctcgcg
     1501 cgtatactgc gtcaccacag cgctgagcgt ggcccgaggg atccgaagct gcttccgcgt
     1561 cagaccgtgc tccgcaagct gcttccgcac cagataccgc tcggcgatgg ccagtttctc
     1621 gccgtgcgtg tagcctggca gccgcagaac ctccatccgg tccagcagcg cccgcggaat
     1681 ggtgtccagc acattggctg tggtaatgaa catgacctgc gacaggtcga agtccacatc
     1741 aagatagtgg tcacggaagg tgttgttctg ttcggggtcg agcacctcga gcagagccga
     1801 ggaaggatcg cccctgaagt cagtgccgac cttgtcaatc tcgtcgagca tgaacaccgg
     1861 gttccgcgtg cccgcctgag ccaggccctg gatgatgcga cccgggagcg cccccacgta
     1921 ggtccgccgg tgcccgcgga tctcggcttc gtcccgcatc ccac
//
LOCUS       DAOSPL010000989         1964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_58027_length_1964_cov_2.807229, whole genome shotgun sequence.
ACCESSION   DAOSPL010000989 DAOSPL010000000
VERSION     DAOSPL010000989.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58027_length_1964_cov_2.807229"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1964
                     /locus_tag="PLQ54_16020"
     CDS             <1..>1964
                     /locus_tag="PLQ54_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94826.1"
                     /translation="FHDYDHEPFFAYPDNGSWQHGSPAATSENYGWGTVRGMRAAEFG
                     FGTGRYFDAVAFGSRATTEARDADDAIIRAQCVLAQGLQYAKERGIHPCIGFELVGDP
                     TDPATQRLYEKKLRYVLSRYPMADYFWLWQSEGLGGGSGVDPASRLAAYVEHYRGDFA
                     YLGSEDRVAEGVRVMLFVRLAYGIIRQVRPDLPVIVSGWGGDRWMRFSDFYIGLDKLL
                     PPDVIFSALDNIDPSWEPHVSHAYGELSPGRQKWPIPWFESDGGGSRRDQWSSQANVK
                     PFTELCRDALAKGCQGLLGIHWETRGVEEVAAYLAQFAWQPSISYDQFYEGFSRRCYG
                     DRYAHDMAAIHQRLEALGPRWTGGSGQVECGGFQWFSGPGRPMPENLAALAEIRAQVE
                     GLLGRAAPRHRERLRYLLATIDFVTYFDQAALQLEPDGPIDQLIARAELEQAEGKVAE
                     SRATAEEAWRRIAECGLEEAMRAYGQRLTSQGDFGNLATINVKAYAMLERTKARLAKL
                     MPEPEDGPPRGALPSPQLAMRYPAGVIPQGVPLPVRCTAFSDAGRVRVTLRYRAPGRV
                     FAAVAMQPTVGCGFAATIPGSAIGAEGVEFYIEARDGVAPPARLPLSAPETLFTCTAH
                     PYRGPSVYAPVPVTDVAFGQPEARKPLVLE"
BASE COUNT          288 a          644 c          690 g          342 t
ORIGIN      
        1 ttccacgact acgaccacga gccgttcttc gcctaccccg acaacggaag ctggcagcac
       61 ggttcgccgg cggcgacatc cgagaactac ggctggggca cggtacgcgg tatgcgggcc
      121 gccgagttcg gcttcggcac cgggcggtac ttcgatgccg tggccttcgg cagccgcgcc
      181 accacagagg cccgggacgc cgacgatgcc atcatccggg ctcagtgtgt tcttgctcag
      241 ggcctgcagt acgccaagga gcgcgggatt cacccgtgca tcggcttcga gctggtgggc
      301 gatcccacag atcccgcgac ccagagattg tatgagaaga aactgcgcta tgtgctctct
      361 cgctatccca tggccgacta cttctggctg tggcagtccg aaggcctggg cggagggagt
      421 ggcgttgatc ccgcctcgcg tctcgcggcc tacgtcgagc actaccgggg tgatttcgcc
      481 tatctgggct cggaggatcg cgttgccgaa ggggtccggg tcatgctgtt cgtccggctg
      541 gcctatggga tcatccggca ggtgcggccc gacctgcccg tgatcgtcag tgggtggggc
      601 ggtgaccggt ggatgcggtt ctccgacttc tacatcggcc tcgacaagct gctgccgccc
      661 gatgtcatct tctcggccct cgacaacatc gatccgtcgt gggagcccca tgtgagccac
      721 gcctacggcg agctgtcgcc gggccggcag aagtggccga tcccctggtt cgagtcggac
      781 ggcggcgggt cgcggcgcga ccaatggagc agccaggcca acgtgaagcc cttcaccgag
      841 ctctgccgcg atgcgctggc caagggctgc caggggcttc tgggcatcca ctgggagacc
      901 cgaggtgtcg aggaagtagc agcttacctc gcccagttcg cctggcagcc tagcatctca
      961 tatgaccagt tctatgaagg gttttcccgg cgatgttacg gtgatcggta tgcccatgat
     1021 atggcagcca tccaccagcg tctggaggcg ctcgggccac gctggaccgg tggctcaggc
     1081 caggtggagt gcgggggttt ccagtggttc tccggccccg gacggccgat gccggagaac
     1141 ctcgctgcgc tggccgagat ccgtgcccag gttgaggggc tgctgggtcg ggcggcgcct
     1201 cggcaccggg agaggcttcg gtacctgctg gccacgatcg actttgtgac atacttcgac
     1261 caagccgcgc tccagctcga acccgacggc ccgatcgacc agctcatcgc tcgcgccgag
     1321 ctggagcagg ccgagggcaa ggtggccgag tcgcgggcca cggcggagga agcctggcgg
     1381 cggatcgccg agtgtggtct cgaggaggcc atgagggcct atggccagcg tctgaccagt
     1441 cagggcgact tcgggaacct tgccaccatt aatgtgaagg cctacgcgat gctcgagcgc
     1501 acgaaggcgc ggctggcgaa gctgatgccg gagcccgagg acgggcctcc gcgtggcgcg
     1561 ctcccgtcgc cccagctcgc catgcggtac ccggcggggg tcatccccca gggcgtgccg
     1621 ctgccggtgc ggtgcaccgc attcagcgac gcgggccgcg tgcgggtcac gctgcgctac
     1681 cgggctcccg gccgggtgtt tgcggccgtg gccatgcagc ccaccgtggg ctgcgggttc
     1741 gccgcgacca ttccgggctc ggccatcggt gcggagggcg tcgagttcta catcgaggcg
     1801 cgggatggcg tcgcgccgcc ggcgcggctg cctctctcgg cgccggagac attgttcacg
     1861 tgtaccgccc atccctatcg tgggcccagc gtctacgcac ccgttcccgt gaccgatgtg
     1921 gcctttggcc agcccgaggc ccgcaagccg ctggtgttgg agtg
//
LOCUS       DAOSPL010000990         1963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_58082_length_1963_cov_2.265199, whole genome shotgun sequence.
ACCESSION   DAOSPL010000990 DAOSPL010000000
VERSION     DAOSPL010000990.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58082_length_1963_cov_2.265199"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PLQ54_16025"
     CDS             complement(<1..613)
                     /locus_tag="PLQ54_16025"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOS94827.1"
                     /translation="MLCLPVLCMILAGAGEFSPPNLVRNPGFEDVRDGVPADWSGERD
                     VYSTAESVVHSGRRALHYSNADPGRYVLCTQTIPLEPGKRYELRFWAKSKDLVGDDSG
                     ATVCLEWSDASGKWVGGYYPGGVKGTTDWTLKVGQTPRIPPEAARCTVSCYVRQKMTG
                     EAWWDDIEVRQYRGPIMSAVLLRPNYRGLLAATERSAAVRVALN"
     gene            complement(627..1889)
                     /locus_tag="PLQ54_16030"
     CDS             complement(627..1889)
                     /locus_tag="PLQ54_16030"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOS94828.1"
                     /translation="MLCLLASAPWASAQDADDAIPVTPARTYAGLEPFDTMMVDFIRA
                     RGIPGAALAVTRNGRLILATGYGWADRDAREPVRPDSLFRIASASKPITGAAVLRLIE
                     QYPGKISLDTRVLDVVDVGPPPGSGSTDVRWRDVTIRHLLHHTGGWDRDTGYDPMFMP
                     LRFARELGIAPPAMPEDIIRCMLGRPLDFDPGTREVYSNFGYCLLGRVIERVSGHSYE
                     EYVREEILGPLGITRMRVGRTLLEGRTDGEVRYYDPGIGDNVFTEDVDEVAAPYGTFC
                     LEAMDAHGGWIASAVDLVRFACAFDHPERCPVLAPASVAELFARPDGPAGYEADGTAK
                     AAYYADGWLVRPTADGRANHWHNGSLPGTYSLLVRRHDGLNWAVIFNQRTCPEGLDVE
                     VIDPALHMAADAVKRWPGDDLFGTFGAT"
BASE COUNT          327 a          695 c          640 g          301 t
ORIGIN      
        1 agttcagcgc cacacggacc gcggccgacc tctccgtggc ggcgagcaga ccgcggtagt
       61 tgggtcgcag gagcaccgct gacatgattg ggccacggta ctgccgcacc tcgatgtcgt
      121 cccaccacgc ctcgccggtc atcttctggc ggacatagca ggaaaccgta caccgagcgg
      181 cctcgggcgg gattcgcggc gtctgcccga ccttgagcgt ccagtcggtc gtacccttca
      241 ccccgcccgg gtagtagccc cccacccatt tcccggacgc gtcggaccac tcaagacaca
      301 ccgtggctcc gctgtcgtcg ccaaccaggt ccttcgactt ggcccaaaac cgcagctcgt
      361 aacgcttgcc gggttccagc gggatcgtct gcgtgcagag cacgtagcgc cccgggtcgg
      421 cattcgagta gtgcaaggct cggcggccgg agtgaacgac ggactcggca gtggagtata
      481 catcccgctc accggaccag tcggccggga ccccgtcgcg cacgtcctcg aagcccgggt
      541 tccggacgag gttcggcggg ctgaactcgc ccgcaccggc caggatcatg cagaggacag
      601 gaaggcacag catcgcggag ctcctctcag gtcgcgccga acgtgccgaa caggtcgtct
      661 cccggccagc gcttcaccgc gtcggctgcc atgtgcaggg ccgggtcgat cacttcgacg
      721 tccagccctt cggggcaggt tcgctggttg aagatcaccg cccagttcag gccatcgtgc
      781 cgccgcacga gcagcgagta ggtaccgggc agcgaaccat tgtgccaatg gttggctctg
      841 ccgtcggcgg tgggacgcac gagccacccg tccgcgtagt aggccgcctt ggctgtgccg
      901 tccgcctcgt accccgccgg gccgtccggc cttgcgaaga gctcggccac gctggccggg
      961 gccagcacgg ggcaccgttc cgggtggtcg aaggcacacg cgaaccgcac caggtcgacg
     1021 gccgaagcga tccagccgcc gtgcgcgtcc atcgcctcca ggcagaaggt gccgtaaggc
     1081 gcggccactt cgtccacgtc ctcggtgaac acgttgtcgc cgatcccggg gtcgtagtac
     1141 cggacttcgc catcggttcg gccctcgagc agcgttcgcc cgacgcgcat gcgtgtgatg
     1201 ccaagcggcc cgaggatttc ctcacgaacg tactcctcat atgagtgccc cgacacgcgc
     1261 tcgatcacac ggcccaagag gcagtagccg aagttcgagt agacctcacg cgtgcccggg
     1321 tcgaagtcca gcggccgccc gagcatgcac cggatgatgt cttctggcat agccggcggc
     1381 gcgatgccga gttcgcgcgc gaaccgaagc ggcatgaaca tggggtcgta ccccgtgtcg
     1441 cggtcccacc cgcccgtgtg gtgcagcaga tgccggatgg tcacgtcgcg ccaccgcacg
     1501 tccgtcgaac cgctgccggg cggaggccct acatccacaa cgtcgagaac ccgggtgtcc
     1561 agggagatct ttcccggata ctgctcgatg agcctgagca cggccgcgcc cgtgatcggc
     1621 ttcgacgcac tggcgatacg aaagagtgag tcgggccgca ccggctcgcg cgcgtctcga
     1681 tcagcccagc cgtatcccgt ggccaggatc agtctcccgt tgcgcgtcac tgccagggcg
     1741 gcgcccggga ttccccgagc ccggatgaag tcgaccatca tggtgtcgaa gggctcgagc
     1801 cccgcgtagg tccgggcggg cgtcacggga atggcgtcgt cggcgtcctg agccgaggcc
     1861 cagggcgccg acgcgaggag gcagagcacc agccccgccc tacttctcga cagcaaagcg
     1921 gatcccgaca tcgatgtact gcccgcccgt cacgtcaacg agc
//
LOCUS       DAOSPL010000991         1959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_58239_length_1959_cov_3.336134, whole genome shotgun sequence.
ACCESSION   DAOSPL010000991 DAOSPL010000000
VERSION     DAOSPL010000991.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58239_length_1959_cov_3.336134"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PLQ54_16035"
     CDS             complement(<1..622)
                     /locus_tag="PLQ54_16035"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS94829.1"
                     /translation="MNRRDFLARSGRAAAGVAAGLTILKGRAVAQNDTIGVAVAGIHG
                     RGVSHIEAFSRMEGVRVVALCDPDSSLFADRAAIAEKAQGSRPRTYQDIREALDDPEV
                     DAVSIATCNHWHALMTVWACQAGKDVYCEKPVSWSVWEGRKAVEAARKYQRIVAGGHQ
                     SRSEKKKRDAIARLWAGQLGGPCYLSRALCFKPRGSIGFKEPKDPPD"
     gene            783..>1959
                     /locus_tag="PLQ54_16040"
     CDS             783..>1959
                     /locus_tag="PLQ54_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94830.1"
                     /translation="MVFSSAMGVVHLAVAAVPPTVGRPVWEAQWVWGAAEPHGPLGLF
                     RKTFDVDGELVLAIIQCSGDDGFTLYVDGDEVFGGGFWWNTTNTVDVTARLSPGRHVV
                     AARVQNAAHPGGLLVQATLVYGDGRRVTLASDDTWRFSAEETPGWLDPGFDDSGWEHC
                     RAYGSPPVGPWHELPLAAVGPVVPGRIVELRCPGQVRAGEDLPVSFAVELSEPTKANH
                     LGYVRLLRDGRVVAEAEDLAPHRPTSGLTAGTAAQVGPAPLRIPRFAWPGDYTVEAGL
                     YGVTLHDAATATRQLHVEARPGAGRVTRAEVRDHRGAPALFLDGKPAYPMWFFSWAPQ
                     PVDVLAFEKAGVNVHTFGVPLGWSGPGRYDHSDVDRLMLQLLGASDQAYAVPRVQMDA
                     "
BASE COUNT          258 a          673 c          699 g          329 t
ORIGIN      
        1 cgtcgggcgg atccttgggc tccttgaaac cgatggagcc gcggggcttg aagcacagag
       61 cgcgcgagag gtagcagggg ccgccgagct ggccggccca gaggcgggca atggcgtcgc
      121 gcttcttctt ctccgaccgg ctctggtgcc ctccggcgac gatgcgctgg tacttgcggg
      181 cggcctcgac cgccttgcgc ccctcccaca cgctccagga gaccggcttc tcgcagtaga
      241 cgtctttgcc tgcctggcaa gcccacacgg tcatcagcgc atgccagtgg ttgcacgtgg
      301 caatggacac cgcgtcgacc tccggatcgt ccagggcctc gcgaatgtct tgataggtcc
      361 tgggtcggct gccctgcgcc ttttcggcga tggccgcgcg gtcggcgaag aggctggagt
      421 cgggatcgca cagcgccacg acgcggacgc cctccatgcg gctgaacgcc tcgatgtggc
      481 tgacacctcg gccatggatg ccagccacgg ccacgccgat ggtgtcgttc tgcgcaacgg
      541 cccggccttt gaggatcgtg agccccgccg ccacgcctgc ggctgcccgt ccggaccgcg
      601 caaggaagtc ccggcgattc atcgtttcca cgacctgaca cctccgctga tggatcctcg
      661 tctggcttct gacttcgttg ggcggcgcgc ggcccccttc gcccggtcgg gcaggggcag
      721 ctgggccccg cgccgaacca cggcatcgag cgcgtgtgtc tcacgccatg gaggacgagg
      781 gtatggtctt ctcgtcggcg atgggcgttg tgcacctggc cgtggccgcg gtgccgccca
      841 ctgttgggag acccgtttgg gaggcccagt gggtctgggg cgcggccgag ccgcacggac
      901 ctctgggatt gttccgcaag acgttcgatg tggatggcga gttggtcctc gcgatcatcc
      961 agtgctccgg cgacgacggg ttcacgctct atgtggacgg tgacgaggtg ttcggcggcg
     1021 ggttctggtg gaacaccact aacaccgtgg atgtgaccgc ccggctgagt cccgggcggc
     1081 acgtggtcgc ggctcgcgtg cagaacgcgg cacatcctgg cggtctcctg gtgcaggcca
     1141 cgcttgtcta cggggatggt cggcgagtca cccttgcgtc cgacgacaca tggcgcttca
     1201 gcgcggagga gacgccgggc tggctcgacc ccggcttcga cgactccggc tgggagcact
     1261 gccgagcata cggcagcccg cccgtcggcc cgtggcacga gctacccctc gccgcggtcg
     1321 gaccggtggt gccggggcgg atcgtcgaac tccgatgccc gggccaggtt cgtgcaggcg
     1381 aggaccttcc ggtgtcgttc gccgtcgagc tctcggagcc gacgaaggcg aaccatctgg
     1441 gctatgtgcg gctcctgcgg gacggccggg ttgttgctga agccgaggac ctggcacccc
     1501 atcggcccac ttcggggctg acggcaggca cggccgcgca agtggggccg gctccgctgc
     1561 gcatcccccg gttcgcctgg ccgggggact acacggttga ggccggcctg tatggcgtga
     1621 ccctccatga cgcggcgacg gcgacgcgcc agctgcacgt cgaggcccgc cccggagcgg
     1681 ggcgggtcac gcgtgccgag gtcagagacc accgcggggc gccggccctg ttcctcgatg
     1741 gcaagcccgc ctatcccatg tggttcttct cctgggcccc gcagcccgtc gatgtgctcg
     1801 cgttcgagaa ggcgggcgtc aacgtgcaca ccttcggcgt cccgctgggc tggtccggcc
     1861 cggggcggta tgaccactcc gacgtcgacc ggctcatgct gcagctgctc ggggccagcg
     1921 accaggccta cgccgtgccc cgggtccaga tggacgccc
//
LOCUS       DAOSPL010000992         1959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_58251_length_1959_cov_2.568803, whole genome shotgun sequence.
ACCESSION   DAOSPL010000992 DAOSPL010000000
VERSION     DAOSPL010000992.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58251_length_1959_cov_2.568803"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /gene="rpmI"
                     /locus_tag="PLQ54_16045"
     CDS             <1..304
                     /gene="rpmI"
                     /locus_tag="PLQ54_16045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOS94831.1"
                     /translation="NRGAGSCHGCPFARGERGPRVRLGTGLMGAKEWLDMPKMKTNSR
                     AKKTFRATGGGKLIHGAANNGHFKVKKSGSRKRRLSKGSAVGKSDSRLVRTMVPYL"
     gene            377..736
                     /gene="rplT"
                     /locus_tag="PLQ54_16050"
     CDS             377..736
                     /gene="rplT"
                     /locus_tag="PLQ54_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOS94832.1"
                     /translation="MARVKRGMTAHRRHRKIIKQAKGYWGRRHNLFKSAKEAVMHAGR
                     YAYRDRRQRKRDMRQLWIARINAGTRALGMPYSRFIAGLKKAGIELDRKVLADLAVTD
                     PEAFAQIVKTAEEALAA"
     gene            782..1621
                     /locus_tag="PLQ54_16055"
     CDS             782..1621
                     /locus_tag="PLQ54_16055"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOS94833.1"
                     /translation="MSEPTIITSRQNPAIKLLRGLLSQRKARQREGLFAVEGDRLVGE
                     ALRAGWPVHTVLLRRPARDEHAAESLATDCRQAGARLIACAADVLDSALDVDASVDAV
                     GLLHLPQGNAPLATEGPLAAFVLEDVRDPGNLGAILRTAHAAGMSGVVLAGHCADPFS
                     PKAVRAACGALFRLPLVAVLEPEAVRAVLPRARHWAACAGGGEAWHCVDVRGPSALWL
                     GNEAHGLSDRAVELCDGRIHVPIAPAAESLNVASTAAVLAFELLRQRRSAAAAVGPTG
                     ETL"
     gene            1618..>1959
                     /locus_tag="PLQ54_16060"
     CDS             1618..>1959
                     /locus_tag="PLQ54_16060"
                     /inference="COORDINATES: protein motif:HMM:NF014909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOS94834.1"
                     /translation="MSAQIDALVAQLLGEISAAGSTAELADVERRALGKKGELTLLLR
                     GMKDVPAHERPEFGRRVNEAKAQVSEAIERCRATLEAAEASRAAEHDRLDVTLPGRGR
                     RPGRPHPMARVL"
BASE COUNT          314 a          666 c          694 g          285 t
ORIGIN      
        1 caaccggggg gctggttcgt gtcacggttg cccgttcgcc cgcggcgagc gcgggccccg
       61 tgtgagactg ggtaccgggt tgatgggtgc caaggagtgg ctggatatgc cgaagatgaa
      121 gaccaattct agggccaaga agacgttccg ggcgacgggt ggaggcaagc tgatccacgg
      181 cgccgccaac aacggtcact tcaaggtcaa gaagtccggg agccgcaagc ggcggctgag
      241 caaaggctct gcggtgggga agtcggacag ccgcctggtg cggacgatgg tgccctacct
      301 ctagcggcgc gcagagggag acaggcaggc ctctgggccc taccggactg cgaggccggg
      361 gaagcgagga gacaccatgg cacgagtgaa gcgcggcatg accgcccacc ggcggcaccg
      421 caagatcatc aagcaggcca agggatactg gggccggcga cacaacctgt tcaagagcgc
      481 caaagaggcg gtcatgcatg cgggtcgcta cgcgtaccgc gaccggcgtc agcgtaagcg
      541 cgacatgcgt cagctgtgga tcgcccggat caacgcgggc acgcgggccc tcggcatgcc
      601 ctacagccgg ttcatcgccg ggctcaagaa ggcagggatc gaactcgacc gcaaggtcct
      661 cgcagatctc gccgtcaccg accccgaagc gttcgctcag atcgtgaaga ccgcagagga
      721 ggccctggca gcctagcgga agccgactgc gtgcggcgct ccaggcctcc cggaccatac
      781 cgtgtccgag cctacgatca tcacgagcag acagaaccca gccatcaagc tcctccgcgg
      841 tctgctgagc caacgcaagg ctcgccagcg agaggggctt tttgcggttg agggcgaccg
      901 cttggtcggc gaggcgctgc gcgccggctg gccggtccac acggtgctcc tgcggcgtcc
      961 cgcccgcgac gagcacgcgg ccgagtctct ggccacggac tgtcgtcagg cgggcgctcg
     1021 ccttatcgcc tgcgccgccg acgtgctcga ctctgcgctc gacgtcgatg cctcggtcga
     1081 tgccgtgggg ctgttgcacc taccccaggg caacgccccc cttgcgacgg agggcccgct
     1141 ggcggccttc gtcctcgagg atgtccgcga tccgggcaac ctgggcgcga ttctccgcac
     1201 agcgcacgcg gcgggcatgt cgggtgtggt cctcgccggc cactgcgccg atcccttctc
     1261 gccgaaggcc gtgcgggcgg cctgtggagc ccttttccgg ctgccactgg tcgcggttct
     1321 ggagccggaa gcagttcggg ccgtcctccc gagggcgcgc cattgggcgg cctgcgccgg
     1381 tggcggcgag gcctggcact gtgttgacgt ccgcgggccc agcgcgctat ggttgggcaa
     1441 cgaagcccac gggctctccg atcgggctgt ggagttgtgc gacgggcgca tccacgtgcc
     1501 gatcgcccct gccgccgagt ccctcaatgt ggcttccacg gccgccgtct tggccttcga
     1561 gctcctgcgg caacgccgca gtgccgccgc cgcggttggc cctacagggg agacgttatg
     1621 agcgctcaga tcgacgcgct agtggcccag ctgctgggcg agatcagcgc ggccggctcg
     1681 accgcagagc ttgcggacgt cgagcggcgc gccctcggca agaaggggga actcactctt
     1741 ctgctccggg gtatgaaaga cgtgcccgcc cacgagcgac ccgagttcgg ccgacgcgtc
     1801 aatgaggcca aggcacaggt ctctgaggcc atcgagcgct gccgggccac gctcgaggcc
     1861 gccgaggcca gccgcgccgc cgagcacgac cggttggatg tcactctgcc cgggcgcggc
     1921 cggcggcccg gacgaccaca tcccatggca cgcgtcctc
//
LOCUS       DAOSPL010000993         1956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_58349_length_1956_cov_3.532351, whole genome shotgun sequence.
ACCESSION   DAOSPL010000993 DAOSPL010000000
VERSION     DAOSPL010000993.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58349_length_1956_cov_3.532351"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1956
                     /locus_tag="PLQ54_16065"
     CDS             <1..>1956
                     /locus_tag="PLQ54_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94835.1"
                     /translation="GLRVGETAANAASLPWTIARRYDFATGREPGPDTHVLAIIVHNS
                     EGQGGLWRGPCVLGPPDDLDTSPLGDGWTRVYPHAVKLHHWCPDDYERSLTGRFTVSL
                     TSVDRTREMVPENVTTGGRFLLPPYLVALEGEHGWAGLATLDLPRAEDGLRIEWRDGG
                     LTCPFLLATDPDTHAYGWTFGPRLGILSGASRRDILNGYLQAVPPRSGRPRPAWWSGP
                     EYCTWGDQCYASQECGSDVGSLTEERVRAWLSTLAEKHIEAPLVVLDAGWWQLSQGLV
                     DELHRQGRHVVLWTQPHWQPDTSRHAEWAMRDSAGRPLSYDPNNWILDYTRADVRAHI
                     AEALASYVTASGWNADGIKLDFPYTAAPVWALHADPSWGAGEQYRARVLQFIYSTVKS
                     HRPDALVTLGCANPLFGDVQDVCRLNEDWFDDPDRFRRRAEVVLSLGQWVNCDDWNAY
                     EHYLDTQAVERPVWGTFTLMSAAYRGDHANRRIPLSAEWARRLSAITRLAKRAPVLGG
                     QKCTFDAATGITRREAADGTLVAAALRLSGSDGPARVLVVRAGRSLLMTATASGPVAV
                     PAPWPVARVEAVSHGGSRRRVPFRAEAGELRLEVEDAAGDVSHYEVVPQPEGAASRLK
                     GGAGLVSGRPQGAANERRARPEGDGAHG"
BASE COUNT          291 a          700 c          663 g          302 t
ORIGIN      
        1 cggcctccgc gttggcgaga ccgcggccaa cgccgcttcc ctgccgtgga cgatcgcgcg
       61 ccgctacgac ttcgccacgg gccgcgagcc cggccccgac acgcatgtcc ttgccatcat
      121 cgtgcacaac tcggaaggac agggcggcct atggcgcggg ccctgtgtcc tcggacctcc
      181 ggatgatctc gacacgtcac ccctgggtga cggatggacc cgggtgtacc cccacgcggt
      241 gaagctccac cactggtgcc ccgacgacta cgagcgatcc ctcaccggtc ggttcaccgt
      301 ctctctcacg tccgtcgacc ggacgcgtga gatggtgccg gagaacgtga ccacgggcgg
      361 ccgtttcctg ctccccccct acctcgtcgc cctcgagggg gagcatggct gggcgggtct
      421 cgccactctg gacctgccgc gagccgagga tggactgcgc atcgagtggc gggacggggg
      481 tctgacgtgt ccattcctgc tggccactga tccggacacc catgcatacg gctggacctt
      541 cgggccgcgg ctcggcatcc tgtctggcgc gtccagacgc gacatcctca acggctacct
      601 ccaggccgtc ccgccccgct ccggccgtcc acgccccgcg tggtggagcg gacccgagta
      661 ctgcacgtgg ggcgatcagt gctatgccag tcaggaatgc ggcagcgatg tcggcagcct
      721 gactgaagag cgtgtgcgcg cctggctctc gacgcttgcc gaaaagcaca tcgaggcgcc
      781 actggtcgtg ctggacgcgg ggtggtggca gctctcgcaa gggctcgtgg acgaacttca
      841 tcgccagggc cgtcacgtcg tgctctggac ccagccccat tggcaaccgg acacgtcccg
      901 gcacgccgaa tgggcaatga gggactccgc cggccggccg ttgtcctacg accccaacaa
      961 ctggatcctc gactacacac gcgccgacgt gcgcgcccac atcgccgagg ctctcgcctc
     1021 ctatgtgacc gcgtcgggat ggaacgccga tggcatcaaa ctggacttcc cctacaccgc
     1081 agctccggtc tgggcactcc acgccgaccc ctcatggggc gcgggcgagc agtaccgagc
     1141 ccgggtgctg cagttcatct actccacggt gaagtcccac cggcccgacg ctctggtcac
     1201 actcgggtgc gcgaaccctc tgttcggcga cgtgcaggac gtgtgtcggc tcaacgagga
     1261 ctggttcgat gatccggacc ggttccgccg ccgcgccgag gtcgtgctgt ccctgggcca
     1321 gtgggtcaac tgcgacgact ggaacgccta cgagcactac ctcgacacgc aggcggtcga
     1381 acgccccgtg tggggcactt tcacgctgat gagtgcggcc taccgaggcg atcacgccaa
     1441 ccgtcgcatc cccctcagcg cggaatgggc gcgccgcttg agcgcgatca cccgcctggc
     1501 caagcgggcg cccgtgctgg ggggccagaa gtgcaccttc gacgcggcta cgggaatcac
     1561 ccggcgtgag gctgccgatg gcacgctcgt ggcagctgcg ctccggctga gcggcagtga
     1621 cggtccggcg cgtgtgctcg tcgtccgcgc cggccggtca ctgctgatga ccgccacggc
     1681 ctccgggccc gtcgcggtac cggcgccatg gcccgtggcc cgagtcgagg ccgtcagcca
     1741 cggtggctca cgccggcgtg tgcccttccg tgccgaagcg ggagagctcc gactcgaggt
     1801 cgaagacgcg gccggcgacg tctcgcacta tgaggtcgtg ccacagccag agggggcggc
     1861 gtcccggctg aagggtgggg ctgggttggt ctccggtcgg ccgcagggcg cagcgaacga
     1921 gcgccgcgca cggcctgaag gcgatggggc ccacgg
//
LOCUS       DAOSPL010000994         1956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_58361_length_1956_cov_2.622830, whole genome shotgun sequence.
ACCESSION   DAOSPL010000994 DAOSPL010000000
VERSION     DAOSPL010000994.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58361_length_1956_cov_2.622830"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..673)
                     /locus_tag="PLQ54_16070"
     CDS             complement(122..673)
                     /locus_tag="PLQ54_16070"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOS94836.1"
                     /translation="MAGAADDKLRVRVERLSGQRVSDCYQCGKCSGNCPVAPEMDYLP
                     SQLMRLVQSGRTAEALASRAIWLCDGCLTCSSRCPRDIDVARVIDGLRAISRRDLDEM
                     GIFAEAFLSGIRRMGRANELPLGLMFNLRRRKPLDNSDMALKLFRRGKLPLIPRRAGS
                     LSAVFERVGRLESGPQAEERTEP"
     gene            complement(676..1623)
                     /locus_tag="PLQ54_16075"
     CDS             complement(676..1623)
                     /locus_tag="PLQ54_16075"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOS94837.1"
                     /translation="MSSEPQNGQCITGSRLEKVLRAGHFAVCGEMGPPQSANTDLIRK
                     KSAYFRGVVDAVNLTDNQTAVVRMSSIASAHIALDEGLEPVIQMTCRDRNRIALQSDL
                     LGAHALGIRNVLCLSGDHQSMGNHPQARGVYDLDSIQLVRAARQLRDEAKFVCGEETK
                     TPPALFIAAAANPFGDPFEARVHRLAKKIAAGADFIQTQAIYDLPRFARFMELVRAQG
                     LHEKAFILAGVLPVKSARSLLFMKNDVAGMSIPDELIERMQGAEDPAKEGVKVAVELI
                     QQLRAIEGVAGVHVMPAMWEQVMPEIAEAAGLLPRPEVA"
     gene            complement(1616..>1956)
                     /locus_tag="PLQ54_16080"
     CDS             complement(1616..>1956)
                     /locus_tag="PLQ54_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOS94838.1"
                     /translation="GLNTTFYGAHVGGGVFEERCGGCGDCVLDRTGGICPIIRCAKSI
                     LNGPCGGSQNGKCEISKETDCAWQLIWDRLNAQGRLARYLEIEPVKDWRPATAGGPRR
                     LVQEVLLNDE"
BASE COUNT          339 a          720 c          578 g          319 t
ORIGIN      
        1 cagcacgtcc agcccgggat agtctcgagc tcgatgtcca gcgcccgaca cgcggcgaga
       61 agcgacgatc cgtagtcgcg actcgtgcca tggagcgcac agccaggata gagggcgtac
      121 ttcacggctc cgtcctctcc tccgcctgag gccccgactc caggcggccc acccgctcaa
      181 agacggccga gaggctcccc gcgcgccgag ggatcaacgg caacttgccc ctgcggaaca
      241 gcttcagcgc catatccgag ttgtccagcg gcttgcgacg gcgcaggttg aacatcagac
      301 cgagaggcaa ctcattggcc cggcccatcc gccggatgcc actgaggaat gcctcggcga
      361 agatccccat ctcgtccaga tcccgccgcg agatggctcg gagcccgtcg atgacccgcg
      421 ccacgtcgat gtccctcggg caacggctgc tgcaggtcag acagccgtcg cagagccaaa
      481 tggcccgcga cgccagagcc tcggccgtcc gccccgactg aacgagccgc atcaactggc
      541 tgggaagata gtccatctcg ggcgccaccg gacagttgcc ggagcacttg ccacattgat
      601 agcagtcgct cactcgctga ccgctgagcc gctccacgcg gacccgcagc ttgtcatcag
      661 cggcgccggc catgcctacg ccacctccgg acggggcaga agccccgccg cttccgcgat
      721 ttccggcatg acctgctccc acatggccgg catgacatgc accccggcaa caccttcgat
      781 ggcgcggagc tgctggatga gctccaccgc caccttcacg ccctccttcg ccgggtcctc
      841 ggcgccctgc atccgctcga tcagctcatc ggggatgctc atgccggcca cgtcgttctt
      901 catgaacagc aacgatcggg ccgacttcac gggaagcact ccggcgagga tgaacgcctt
      961 ctcgtgaagc ccctgcgcgc gcacgagctc catgaaacga gcgaagcggg gcagatcgta
     1021 gatggcctgg gtctggatga aatcggcccc cgccgcgatc ttcttcgcga gccggtgaac
     1081 tcgcgcctcg aacgggtcgc cgaaagggtt cgctgcggcc gcgatgaaca acgccggcgg
     1141 cgtcttcgtc tcttcgccac acacgaactt ggcttcgtcg cgaagctgcc gagccgcccg
     1201 aacgagctgg atcgagtcca ggtcatacac gccacgagcc tgagggtgat tgcccatgct
     1261 ctggtggtcg cccgataggc acagcacatt acgaatcccc agcgcgtgcg cgccaagcag
     1321 gtcgctctgc agggcgatgc ggttccggtc gcggcacgtc atctggatga ccggctccag
     1381 cccctcgtcc agggcgatgt gcgccgacgc gatgctcgac atccgcacca cggccgtctg
     1441 gttgtccgtt aggttcacgg cgtcaaccac gccccggaag taagccgact tcttgcggat
     1501 caggtccgtg ttggcgctct gcggcggccc catctcgccg cacacggcaa agtgccccgc
     1561 gcggagcacc ttctccagcc gactcccggt gatgcattgc ccgttctgcg gctcgctact
     1621 catcgttgag caacacttcc tgcacgaggc gacgcggccc cccggccgtt gccggacgcc
     1681 agtccttcac cggctcgatc tcgagatacc gcgccagccg cccctgggcg ttgagccggt
     1741 cccagatcag ctgccaagcg cagtcggtct ccttgctgat ctcgcacttg ccgttctgac
     1801 taccaccaca ggggccgttg aggatgctct tggcgcaacg gataatgggg cagatccccc
     1861 ctgtgcggtc cagcacgcag tcaccgcacc ccccacaccg ctcctcgaac acacccccgc
     1921 ccacgtgagc gccgtagaat gtcgtgttca gtcccg
//
LOCUS       DAOSPL010000995         1954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_58439_length_1954_cov_2.968404, whole genome shotgun sequence.
ACCESSION   DAOSPL010000995 DAOSPL010000000
VERSION     DAOSPL010000995.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58439_length_1954_cov_2.968404"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..539)
                     /locus_tag="PLQ54_16085"
     CDS             complement(63..539)
                     /locus_tag="PLQ54_16085"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOS94839.1"
                     /translation="MEEQGIRDSVTGVYSRLYFEEQLELEVLRGIRRGVAFSILLVEI
                     DGFEAFGARHGDSAIEGLLLHIAEIARDRLRGADRLFRFGDHGFVAILPETPRANAAI
                     PAEGIREAVSQADPSELVPEAPNARVTATVTVVGFPDDGEDVTTLMGKLLQRSSSS"
     gene            976..>1954
                     /locus_tag="PLQ54_16090"
     CDS             976..>1954
                     /locus_tag="PLQ54_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94840.1"
                     /translation="MARTLARLTVLVALLAGSAAWAQFTPAPRISMTRQDDSWFVSGE
                     AYRAVVAADGCLTNLAVNGTEFLAPGIGVSRGSYFYRNAPLRLPRVSRVPGNQIVAEG
                     DQASIRYGFGLDRMTWTLTNRTDTKLNFYLVLDRSVRAGRNGQGQWARTPVNARWEHS
                     VWYRDAAFLGVSGATSVWGPWGDGNQVVDVTLSPNEVRRVELTMGTANRDERATADEV
                     ARQPDSAAGGGIERDRDQGRRRDLTILSPRDLQVFQRQSRREGPMLISGRVSVEADTV
                     EARIEGRSAGGPVSSRWFEVPFVPDTGAFNGTVTQPAGGWYRLQVRALRD"
BASE COUNT          294 a          621 c          724 g          315 t
ORIGIN      
        1 ccttccctgg cgggcacgcg acgggctccc ttcgcatggg gtacacgagg tgggcgggcc
       61 gcctagctac tgctggaacg ctggaggagc ttgcccatca gcgtcgttac gtcctcgccg
      121 tcgtccggga agccgacgac cgtgacggta gcggtcacgc gggcgttggg agcctcgggg
      181 acgagttccg acgggtcggc ctggctcacg gcctcgcgga taccctcggc cgggatcgcc
      241 gcattggccc tgggggtctc cgggagaatc gcgacgaagc catggtcacc gaagcggaac
      301 aggcggtcgg cgccgcgcag ccggtcgcgc gcgatctcgg cgatatggag caacaatccc
      361 tcgatggcgc tgtcgccatg tcgggcgccg aaggcctcga agccgtcgat ctcgacgagc
      421 agaatgctga aggcaacacc tcggcggatg cctcgaagca cctccagctc gagctgctcc
      481 tcgaagtaga gccggctgta gacgccggtc acgctgtcgc ggatgccctg ctcttccaca
      541 ctcagtacct cccgggcggc caacgcaggc cggcgagcgg ccacgctggc agtataccat
      601 cgaagagttc ggctccggct caggcgagcg aatgttggtg tctcgggcat cccgttcggc
      661 ccggcggcag ggccatgcgt cattcgagga ccggccggac ggggctcttc acgcgtcgcg
      721 agcccttcct cctggcccct cctgggtggt tggaggagct tcggggtagt cgcacaggcg
      781 gcgataggcg gtgggccggt ggtgccttgt ccggcccgag gccatcggca cccgaggagg
      841 ctctcggtcg cgtcggtggg ccaagggacc gtgggcgcga tgtccccacc cttgacgtcc
      901 gcccagcatc gggtgttcgc tagtgccggt ggcctgcagg tgcgcacatg ggcgttgact
      961 tggaggcggt acgcgatggc tcggactctg gcacggctca ccgttctggt ggcgcttctg
     1021 gcgggcagcg ctgcttgggc ccagttcacc ccggcgcccc ggatcagcat gacccggcag
     1081 gatgactcgt ggtttgtgtc gggggaggcg taccgcgccg tggtggcggc cgatggctgc
     1141 ctgacgaacc tggccgtcaa cggaaccgag ttcctggcgc ccgggatcgg cgtctcacgc
     1201 ggcagctact tctacaggaa cgcccctcta cggctgcccc gcgtcagccg agtgcccgga
     1261 aaccagattg tggccgaggg ggaccaagcc tcgatccggt atggcttcgg tttggaccgg
     1321 atgacctgga cgctcacgaa ccggaccgac acgaagctga acttctacct ggtgctggac
     1381 cggtccgttc gggccgggcg gaatggccaa ggccagtggg cgcgtacgcc ggtcaacgcg
     1441 cggtgggagc attcggtctg gtaccgagac gccgcgttcc tgggggtatc tggcgcgacc
     1501 agtgtctggg gaccatgggg cgatggcaac caggtggtcg atgtgaccct gagtccgaac
     1561 gaagtgcgga gggttgaact caccatgggg actgcgaaca gggacgaacg ggcaacggca
     1621 gacgaggtgg cgcgtcagcc ggactcggcg gccggcggcg ggatcgagcg agatcgggat
     1681 cagggccgga ggcgggacct gaccatactc tccccgcggg acctgcaggt cttccagcgc
     1741 cagagccggc gcgagggccc gatgctcatc agtggccgcg tcagtgtcga ggccgacacg
     1801 gtcgaggcga ggatcgaggg gcgctcggcc ggcggtccgg tctccagccg gtggttcgag
     1861 gtgcccttcg tgccggatac gggcgcattc aacggcacgg tcacgcagcc agcgggcgga
     1921 tggtaccggc ttcaggtccg cgctctgcgt gatg
//
LOCUS       DAOSPL010000996         1953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_58468_length_1953_cov_3.358272, whole genome shotgun sequence.
ACCESSION   DAOSPL010000996 DAOSPL010000000
VERSION     DAOSPL010000996.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58468_length_1953_cov_3.358272"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PLQ54_16095"
     CDS             complement(<1..663)
                     /locus_tag="PLQ54_16095"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOS94841.1"
                     /translation="MKTRALILALALAGLCISVHVSTGADAPAAGVPDTARVTPSQPK
                     PVATQPVDLAICLDTSGSMSGLIDSAKQKLWAVVNELASATPAPDLRVGLIQYGNDGL
                     DPTTGWVERKLGLTSDLDEVYNQLFALTTNGGQEYVARAITLARESMEWSADPRALKI
                     VFVCGNEPATQDPEITVSQACEAAIAHDIIVNSIYCGPPQSDEALGWQEVAKLADGEY
                     GAI"
     gene            complement(758..>1953)
                     /locus_tag="PLQ54_16100"
     CDS             complement(758..>1953)
                     /locus_tag="PLQ54_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94842.1"
                     /translation="DLDSLDLCGESCASVLRISGSSTVQWQFWAQHARGLHTGGVAMR
                     VPHVGVCALLALASLVGAQDPAPPEVSLRADVDGDGEADRVAIVRVGDTAVKGVSAFA
                     VEVVVGGGVSTADPIEAVGPLEKEPPLPESIGYLQVLPDGPLCVTDLDGDGSPEIIAR
                     LRLPTSRTNVETLVYGYDPAQKSLRGLWGWTSDGVLGMYSEGVPRLLLLPYRWLYPGC
                     PEFGPIFAWRDGKAHRSLESLPPRLAAELPAIYTPRYVPYELRGDPDTLDLYVRGLMA
                     TGENAQAIAAAEDAIGRLDYAESQIAGDRRRALMVLGDARAASGAIDGDVGALEAYRQ
                     AYAMEREEGFASPEGFAALHAALALAAAGDRDGALHWLDEAEKAEGQPGKFAATRERI
                     PPKAE"
BASE COUNT          287 a          695 c          670 g          301 t
ORIGIN      
        1 gatggctccg tactcgccat cagccagctt ggcgacctcc tgccatccca atgcctcatc
       61 ggactgcggg gggccgcaat agatgctgtt gacgatgatg tcgtgggcga tggcggcctc
      121 gcaggcctgg ctgaccgtga tctccgggtc ctgcgtagcc ggttcgttcc cgcacacgaa
      181 gacgatcttg agggcgcgcg ggtcggccga ccactccatc gactcccgtg ccagggtgat
      241 ggcccgggcc acgtactcct gcccgccgtt cgtcgtcagt gcgaagagct ggttgtagac
      301 ctcgtccagg tcggaggtga ggccgagctt gcgttcgacc caccccgtcg tcggatccag
      361 gccgtcgttg ccatactgga tcagcccgac tcgcagatcg ggcgcaggag tggccgacgc
      421 cagttcattg accacagccc acagcttctg cttggccgag tcgatgagcc cgctcatgct
      481 gcccgaggtg tcgaggcaga tggccagatc gacgggctgg gtcgcgaccg gcttaggttg
      541 agacggcgtc acccgcgcgg tgtcgggcac acccgcggcg ggcgcgtcgg cgccggtgga
      601 gacgtggact gaaatgcaga gccccgccag cgccagggcg aggatgagtg cgcgggtctt
      661 catggcatgg acctcccggg tcggcgcgag tgaagggtca cgggaggtag gaccggcgcg
      721 ggcggacggt tgttccagat gggcgggagg ccgagaccta ctcggccttc ggcgggatgc
      781 gctcccgcgt tgccgcgaac ttgccgggct gcccctcggc cttctctgcc tcgtccagcc
      841 agtgcagcgc cccatcgcgg tcgccggccg ccgcgagtgc gagcgccgcg tgcagggccg
      901 cgaagccctc gggagacgcg aagccctcct cgcgctccat cgcgtacgcc tgccggtagg
      961 cctccagcgc gcctacatcg ccatcgatgg cgcctgacgc ggccctggcg tcgccgagca
     1021 ccatcaacgc cctccgccgg tcgccggcga tctggctttc ggcgtagtcc agccgcccga
     1081 tggcgtcctc ggccgccgcg atggcctgag cgttctcgcc cgtcgccatg aggccgcgaa
     1141 cgtacaggtc gagcgtgtcg gggtcgcccc tcagctcgta cggcacgtag cgcggcgtgt
     1201 agatggccgg gagctcggcc gcgagccggg gcggcaggct ctccagtgac cggtgggcct
     1261 tgccgtctcg ccacgcgaag atcgggccga actcggggca gcccgggtag agccaccggt
     1321 atgggagcag gagcaggcgg ggcactccct cggagtacat cccgagcacc ccgtccgacg
     1381 tccatcccca cagacccctg agcgacttct gtgcggggtc gtagccgtag accagcgtct
     1441 cgacgttggt ccggctggtc ggcagccgca gccgcgcgat gatctccggg ctgccgtcgc
     1501 cgtccaggtc ggtcacgcac agcgggccgt cgggcagcac ctgcaggtag ccgatcgact
     1561 cgggcaaggg cggctccttc tcgagcgggc cgacggcctc gatcgggtcg gccgtcgaca
     1621 cgccaccccc aacgaccacc tcgaccgcga atgcgctcac ccccttgact gccgtgtcac
     1681 ctacgcgcac gatcgccact cggtccgcct caccatctcc gtcgacgtcg gcgcgaagcg
     1741 atacctccgg cggcgccggg tcctgcgcgc ccacgagcga ggcgagggcc agcagagcac
     1801 agacaccaac atggggcact cgcatagcta cacctccggt gtgcaagcca cgggcgtgtt
     1861 gggcccagaa ctgccactgc actgtgctgc ttccgctgat gcggagcacg ctcgcgcacg
     1921 actcgccaca caggtccagc gagtcaaggt cca
//
LOCUS       DAOSPL010000997         1952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_58517_length_1952_cov_2.987348, whole genome shotgun sequence.
ACCESSION   DAOSPL010000997 DAOSPL010000000
VERSION     DAOSPL010000997.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58517_length_1952_cov_2.987348"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /locus_tag="PLQ54_16105"
     CDS             <1..1462
                     /locus_tag="PLQ54_16105"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOS94843.1"
                     /translation="TEGVDRDYICDKDGTGVRLGTVYPELVLAPGASRSAVPTFVSIL
                     PGDWHSGLAVYCRWLDGWYQPMSPRQQWFREVFNFRQRFLYWADPLVKPGDVRVDLQP
                     ALDEARAEFGGIEYLHLFDWGNCGPHGRIYGRTGDLDPGDYLPGGWDGLRSGVEAAQA
                     DGVRVGFYIEGYLLEERGELGRAHGKEWQLVGPDGKGLYWPSSTEMFVCPHPEAWREV
                     QETTYERMVRRMSADGMYMDQFGFAGADKRCYSPDHGHPVPSFPAAVERGMLERARAA
                     ISRARDGVVLYTEEIPTDINSQFQDGSFTYSMLSSLGAGARVPISLFRFAVPSFKPIE
                     ILYCDKPTASWATGVKWVFFNGEGIWLEGPAKEWFAPQTRRAITKCHALLREHRDAFT
                     SDHPSPLVPTLNPLVHANRFPSRGKVVYTLYNEAYRTVSGDMLAVPAVAGARYSDAWN
                     GEELTPRTEGDQAIIAARVGPRSVGCIVISGEGVGE"
     gene            1548..>1952
                     /locus_tag="PLQ54_16110"
     CDS             1548..>1952
                     /locus_tag="PLQ54_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94844.1"
                     /translation="MRCWARLVLACACSAAAPLRAVPSLVEETAGGVYTVRDDSGQWG
                     GDLSESVTHQSSAPYAARKILDMSTVSDADWERVREVRLSAHLMVRDYSWHDRPPANG
                     LDEAVQFVINGHVHEVPTARITPVFVEGTAPRP"
BASE COUNT          308 a          609 c          690 g          345 t
ORIGIN      
        1 caccgagggc gtcgatcgtg actacatctg cgacaaggac gggacggggg tcaggctggg
       61 cacggtgtac cccgaactcg tgctggcgcc cggcgcatcg aggagcgcgg tgccgacatt
      121 cgtgagcatc ttgcctggcg actggcacag tgggctggcc gtctactgcc gatggctcga
      181 tgggtggtac cagccgatgt cgccgcgcca acagtggttc cgagaggtct tcaacttccg
      241 tcaaaggttc ctctactggg ctgatccgtt ggtcaaaccc ggcgacgtgc gtgtcgacct
      301 gcaaccggcg ctggacgagg ctcgggccga gttcggcggg atcgagtacc tccacctgtt
      361 cgattggggt aactgcggcc cgcacggccg catctacggc cgtacgggcg acctggaccc
      421 gggagactac ttgcccggtg gctgggatgg cttgaggtcg ggtgttgagg ccgcccaggc
      481 cgatggcgtc cgggtcgggt tctacatcga gggctacctc ctcgaggaac gtggcgagct
      541 tggcagggcc catggcaagg agtggcagct cgtggggccc gacggcaagg ggctctactg
      601 gccgagcagt accgagatgt tcgtctgtcc acaccccgag gcgtggcggg aggtccagga
      661 gaccacctat gagcgcatgg tacgccggat gagcgccgac ggcatgtaca tggaccagtt
      721 cgggttcgcg ggggcagaca agcgctgcta ctcgcccgat cacggtcatc cggtgccctc
      781 attccccgcc gccgtggagc gcgggatgct cgagagggct cgagcggcta tctcccgcgc
      841 gagggatggc gttgtgttgt acaccgagga gatcccgacg gacatcaact cgcagttcca
      901 ggatggcagc ttcacgtaca gcatgctgtc gagcctcggt gcgggcgcac gggtgcccat
      961 cagcctgttc cggttcgccg tgccgtcgtt caagccgatc gagatcctgt actgcgataa
     1021 gccgacggcc tcatgggcca cgggcgtgaa gtgggtgttc ttcaacggcg agggcatctg
     1081 gcttgagggg ccggcgaagg aatggttcgc gccccagacg cggcgggcga tcaccaagtg
     1141 tcacgccctg ctccgcgagc atcgcgatgc cttcacgagc gaccacccga gcccgctggt
     1201 gccgacgttg aaccctctcg tccatgccaa tcggttcccc tcgcgcggga aggtggtcta
     1261 caccctctac aatgaggcct accgcacggt ttcgggtgac atgcttgccg tgcccgccgt
     1321 ggccggagcc cggtactctg acgcatggaa cggtgaggag ctgacgccgc gcaccgaggg
     1381 cgaccaggcc atcatcgccg cccgcgtcgg cccgcggagc gtggggtgta tcgtcatctc
     1441 gggcgagggt gtgggcgagt agggcgaaga gcgcgacagg atcgccgtgg gcccgagggg
     1501 ccatgggggt cgtctggcat cgcctaccct ccttgcggag gtgcgccatg cgctgttggg
     1561 ctcgtctggt tcttgcgtgt gcgtgttcgg ctgcggctcc tcttcgcgcc gtaccatcgt
     1621 tggttgagga gaccgctggt ggtgtctaca cggtacgcga cgactcgggc cagtgggggg
     1681 gcgacctgag cgagagtgtc acacaccagt cgtcagcccc ctacgcggca cgcaagatcc
     1741 tcgatatgag cacggtctcc gacgcggact gggagcgcgt tcgggaggtg aggctctccg
     1801 cccacctcat ggttcgcgac tactcgtggc atgacagacc gccggccaat ggtctggacg
     1861 aggccgtgca gttcgtcatc aatggccacg tccacgaggt accgacagcc cggatcaccc
     1921 ccgtcttcgt cgaggggacg gcgccgaggc ca
//
LOCUS       DAOSPL010000998         1951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_58556_length_1951_cov_3.703059, whole genome shotgun sequence.
ACCESSION   DAOSPL010000998 DAOSPL010000000
VERSION     DAOSPL010000998.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58556_length_1951_cov_3.703059"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PLQ54_16115"
     CDS             <1..370
                     /locus_tag="PLQ54_16115"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HOS94845.1"
                     /translation="IDKQYPHQARKVMHAIWGSGQMMFTKMVVVLDEHVNVQDMGEVI
                     WRLGNNVDWRRDVVIAEGPVDVLDHASPTPNLGGKIGIDATKSWPEEGYPREWPADIA
                     MSAEVVARIDALWATLGIRC"
     gene            370..1083
                     /locus_tag="PLQ54_16120"
     CDS             370..1083
                     /locus_tag="PLQ54_16120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HOS94846.1"
                     /translation="MPGHDTRAMFSRIAGRYDRVNTVLSLGLDEGWRRRSVRRALARP
                     GLDVLDLCAGTGKVALACCTAPHRPRLVVAADFAEPMLEAGHRSASRRGLPVTFVACD
                     CQRLPFRSASFDVATCAFGVRNLVDLEAGLREAARCLRPGGSMVIMEFMNPGQRWARR
                     LVSWYVKKAIPLLGGVLSGSWSAYRYLPDSIDRFLTPPELVRVLLDCGFESVETEERT
                     LGVCSVFYATRGREGWETR"
     gene            1080..1670
                     /locus_tag="PLQ54_16125"
     CDS             1080..1670
                     /locus_tag="PLQ54_16125"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HOS94847.1"
                     /translation="MRFVLAITGASGALYGVRLLYALVERGQEVSLVVSPTGAEIIAQ
                     ETGRSVETGRGLPPLVPAEAKTVTRYHFDDLMAPIASGSVAFDGMVIAPASMGTIGRL
                     AAGTANDLIARAADVCLKERRRLIVVPRESPMSLIHLRNLAALAEAGATIMPACPAFY
                     GRPATLDDLVAGFVGRILDVLGVENDLAVRWGEDRS"
     gene            1667..>1951
                     /locus_tag="PLQ54_16130"
     CDS             1667..>1951
                     /locus_tag="PLQ54_16130"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOS94848.1"
                     /translation="MISYALRRAWAYLEMVKIEHTLFAAPFCLSALLVATGGRPSGRL
                     VVWVVVALTAARSAAMAFNRIVDAHLDALNERTRGRHIPRGVITRGAAIGF"
BASE COUNT          300 a          617 c          689 g          345 t
ORIGIN      
        1 gatcgacaag caataccccc accaggcccg caaggtcatg cacgccatct ggggctcggg
       61 tcagatgatg ttcaccaaga tggttgtcgt gctggatgag cacgtgaacg tgcaggacat
      121 gggcgaggtc atctggcgtc tggggaacaa cgtggactgg cggcgcgatg tggtcatcgc
      181 cgaaggtccg gtggacgttc tcgaccatgc ctcacccacg ccgaatctgg gcggcaagat
      241 cggcatcgat gccaccaagt catggcccga ggagggctat ccacgcgagt ggccggccga
      301 catcgccatg tcggccgagg tggtagccag gatcgacgcc ctgtgggcca cgttggggat
      361 ccgctgctga tgccagggca cgacacgcgg gccatgttct cgcgcatcgc gggccgctac
      421 gaccgcgtga acactgttct gagcctggga ctcgacgagg ggtggaggcg ccggtctgtc
      481 cggcgcgccc tggcacggcc tggcctggac gtcctcgatc tgtgcgccgg caccggcaag
      541 gttgccttgg cgtgctgcac ggcgccccat cggccgcgtc tggtggtggc ggccgacttc
      601 gcggaaccga tgctggaagc gggccaccgg tccgcctcga ggcgcggcct gccggtgacc
      661 ttcgtggcgt gcgactgcca gagacttccg ttccgctcgg catccttcga tgtcgccacc
      721 tgcgccttcg gcgtgcggaa cctggtggac ctcgaagccg gactgcggga ggcggcacgc
      781 tgcctgcggc ccggtggatc gatggtgatc atggagttca tgaacccggg ccaacgatgg
      841 gcgcgccggc tggtatcctg gtatgtgaag aaggcgattc ccctgttggg cggcgtgctg
      901 tcgggctcat ggtcggccta ccgctacctg ccggattcca tagaccggtt cctgacaccg
      961 ccggagctgg ttcgagtact gctcgactgc ggcttcgagt cggtcgagac ggaagaacgg
     1021 accctgggcg tgtgcagcgt gttctacgcg acccgcggtc gtgagggatg ggagacgcga
     1081 tgaggttcgt gttggccatc acgggggcaa gcggcgcgtt gtacggagtc cggctgctct
     1141 atgccctggt ggagcgcggg caggaggtct ctctggtggt atcgcccacg ggcgccgaga
     1201 tcatcgccca ggagacgggc cgttctgtgg agacggggcg cggcctcccg cctctggtac
     1261 ctgccgaggc caagacggtc acacgctacc acttcgatga cctgatggcg cccatcgcca
     1321 gcgggtcggt ggctttcgac gggatggtca tcgctccggc gagcatgggc accatcggcc
     1381 gcctggcggc cggaaccgcg aatgacctca tcgccagggc ggccgacgtg tgcctcaagg
     1441 agcgccggag actcatcgtc gtgccgcgcg aatcgcccat gagcctcatc catttgagga
     1501 acctggccgc attggccgaa gcgggagcga ccatcatgcc cgcatgcccc gcgttctacg
     1561 gccgtccggc caccctcgac gatctggtgg ccgggttcgt cggccgaatc ctcgacgtgc
     1621 tgggtgtcga gaacgacctg gcggtacgct ggggagaaga tcggtcgtga tcagctacgc
     1681 cctacggcga gcctgggcgt atctcgagat ggtgaagatc gaacacacac tcttcgccgc
     1741 gcctttctgt ctgagcgcgc tcctggtcgc caccggaggc cgaccgtctg gacggcttgt
     1801 ggtttgggtc gtagtggcgc tcaccgccgc gcgctcggct gccatggctt tcaaccggat
     1861 cgtggacgcg catctggatg cgctcaacga gcgaacccgg ggacgacaca tcccgcgggg
     1921 agtcatcact cgaggggccg ccatcgggtt c
//
LOCUS       DAOSPL010000999         1949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_58650_length_1949_cov_2.785639, whole genome shotgun sequence.
ACCESSION   DAOSPL010000999 DAOSPL010000000
VERSION     DAOSPL010000999.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58650_length_1949_cov_2.785639"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="PLQ54_16135"
     CDS             complement(<1..1175)
                     /locus_tag="PLQ54_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HOS94849.1"
                     /translation="MMIAMGTLLTLATAPAPAAAELTVHCDAPGFRSSPTLWGLFFEE
                     INHAGDGGLYAELVRNRSFDGPRPLESWRLITEGDGAGEMALDDSRPVSEVSPRSLRL
                     RVDRCRDGRVAVANEGYFGIGARRGEEYDLSFCARRSPDLGGTVRVSLEHASGRVLAQ
                     AEVEGLSTEWGRYACTLRASADCPDARLVIAVSDRGTLWLDHVSLFPRNTWKHRANGL
                     RPDLAAMLADTKPAFLRFPGGCFVEGPDLENAWRWKTTIGDPVERPGHWNLWDYWSTD
                     GLGFHEYLQMCEDLGAEPLFVINCGMACQARNGPAVPMDELEPWVQDALDAIDYANGP
                     VDSTWGRVRARNGHAEPFGLRYVEIGNENWGPGYEERYARFYRAIKERHPEVTLIANA
                     "
     gene            complement(1353..>1949)
                     /locus_tag="PLQ54_16140"
     CDS             complement(1353..>1949)
                     /locus_tag="PLQ54_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOS94850.1"
                     /translation="RPPSSVEKSPRRGGPFKEAFRNRVAFVYGTGGTANENRWSMAKA
                     RYDAEAFWYRANGSVDIVADTEARELGPDRSIILYGNADTNRAWEVLLRDSPVQVRRG
                     SLRVGNRRLEGDDLACLFVRPGASSDTHVGVVAGTGMSGLRLTERMPYFVSGVALPDW
                     TVCRTTLLSRGTEGLVGAGFFGNDWSVEAGESAWAEGA"
BASE COUNT          313 a          715 c          616 g          305 t
ORIGIN      
        1 gcatttgcga tcagggttac ctccgggtgg cgttccttga tggccctgta gaaccgggca
       61 tagcgctcct catagcccgg cccccagttc tcgttcccga tctcgacgta cctgaggccg
      121 aagggctcgg cgtggccgtt ccgcgcccgg acgcgccccc aagtggagtc caccggcccg
      181 ttggcgtaat cgatggcatc gagagcgtcc tgcacccacg gctccagttc gtccatgggc
      241 acggcgggac cgttgcgcgc ttggcacgcc atgccacagt tgatcacaaa cagcggctcg
      301 gcgcccagat cctcacacat ctgcaggtac tcgtggaatc cgagaccatc ggtcgaccag
      361 tagtcccaca ggttccagtg ccccggccgc tcgaccggat cgccgatggt cgtcttccat
      421 cgccacgcgt tctccaggtc gggcccctca acgaagcacc cgcctgggaa gcgcaagaag
      481 gcgggcttgg tatccgctag catggcggcc aggtcgggcc gcaggccgtt ggcgcggtgc
      541 ttccaggtgt tccgcgggaa cagcgacacg tgatcgagcc acagcgttcc gcggtcggac
      601 acagcgatca ccaggcgcgc atcggggcag tcggccgagg cccgcagcgt gcacgcatag
      661 cgtccccact cggtcgatag gccttccacc tccgcttggg ccagcaccct cccggaggcg
      721 tgctcgagtg agactcgaac cgtgccaccc aggtcggggc tgcgccgggc gcagaaggac
      781 aaatcgtact cctcgccccg gcgcgcgcca atgccgaagt agccctcatt ggccaccgcc
      841 actcggccgt cgcggcagcg gtcgacgcgc agtcgcagcg accgtgggct cacctcagag
      901 accggccgag agtcatcgag cgccatctcg ccggccccat cgccctcggt gatgagccgc
      961 cagctttcga gcggccgcgg gccgtcgaag gagcgattcc gtacgagctc ggcgtagagc
     1021 ccgccatcgc cggcgtgatt gatctcctcg aagaacagcc cccagagtgt ggggctggac
     1081 cggaagccgg gcgcatcgca gtgtaccgtc aactccgccg ccgccggcgc cggcgccgtg
     1141 gcgagtgtca gcagcgttcc cattgcgatc atcatcgcag tcctcctgtc aagagccatc
     1201 ccgggaacga gcccgacagg gcggcaccag cctgtggctg gctcgccctg ttcggccccc
     1261 cgcacaccct ccgcacgaca gagggaagac tgcccgggac agggccccgt agcggaaatg
     1321 gcactgtcca gcccactcgg agtgccgtgc ggctatgccc cttccgccca cgccgattcg
     1381 ccggcctcca ccgaccagtc gttcccgaag aagccggctc cgaccagccc ttcggtgcca
     1441 cggctcagca acgtcgtgcg gcagacagtc cagtcgggca gtgccacacc cgaaacgaag
     1501 tagggcattc gttcggtcag ccgcaggccc gacatccccg tgccggcgac cacgcccacg
     1561 tgcgtgtcgc tgcttgcccc gggacgaacg aacaggcacg ccagatcgtc gccctccagt
     1621 ctacggttgc cgacccgcag gctgcctcgc cgcacctgca ccgggctgtc gcggagcagg
     1681 acctcccagg ccctgttggt atccgcattg ccgtagagga tgatgctgcg gtcggggccg
     1741 agctctcggg cctccgtgtc tgccacgatg tcaaccgagc cgttcgcgcg gtaccagaat
     1801 gcctcggcgt catagcgcgc cttggccatc gaccaacggt tttcgttggc cgtgccgccg
     1861 gtgccgtaga cgaacgccac gcggttgcgg aaagcctctt tgaatggccc gccccttcgc
     1921 gggctcttct cgaccgacga cggcggccg
//
LOCUS       DAOSPL010001000         1947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_58717_length_1947_cov_3.823467, whole genome shotgun sequence.
ACCESSION   DAOSPL010001000 DAOSPL010000000
VERSION     DAOSPL010001000.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58717_length_1947_cov_3.823467"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..911)
                     /locus_tag="PLQ54_16145"
     CDS             complement(12..911)
                     /locus_tag="PLQ54_16145"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOS94851.1"
                     /translation="MIDITTYLEERGARVDSYLDTVLPAASHAHGRLYEAVRYSIFAG
                     GKRFRPALCIASCEAFGGLTEAALPAAAALELIHVSSLIHDDLPCMDDSDLRRGRPSC
                     HRAFAESLAVLAGDWLLCFPFALVAEAGESAGVESARTLRLVRELAEAVCRAGIVEGQ
                     AEDLDAEARHISFEALRALHARKTGSLIRAAARCGLIAAGVPLDDVVRFAAYAEPLGL
                     AFQIADDILDVVSDESTLGKPVGADDANQKSTYVSCLGLEGAQEMAARVAVDAHRELD
                     ALPDWVDTRVLRALVDFVVDRRS"
     gene            complement(962..1879)
                     /gene="cysK"
                     /locus_tag="PLQ54_16150"
     CDS             complement(962..1879)
                     /gene="cysK"
                     /locus_tag="PLQ54_16150"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOS94852.1"
                     /translation="MTELVGHTPLVRLNRVTEGCVGTVVGKCEFMNPLSSVKDRIGLS
                     MIEAAEGEGRIGPGSVIIEPTSGNTGIALAWVAAVRGYRCILTMPDSMSLERRALLRA
                     LGAELELTPGAEGMRGAIARAQELAAQIPGSFIPMQFENSANPGVHRATTAEEIWEDT
                     EGQADILVAGVGTGGTLTGCAELIKARRPGFRAIAVEPLASPVLSGGKPGPHKIQGIG
                     AGFVPAVLRTDLVDEIITVASDDAMAMARRLAKEEGILAGISAGANVFAAVGVARRPE
                     NAGKLIVTILCDTGERYLSQAPFAETPPG"
BASE COUNT          324 a          723 c          621 g          279 t
ORIGIN      
        1 gcctccccgg cctagctccg gcggtcgacc acgaagtcga ccagcgcgcg cagcacccgg
       61 gtgtccaccc agtcgggcag ggcgtcgagc tcgcgatggg catcgaccgc cacacgagcc
      121 gccatctcct gagcgccctc gagccccagg catgacacat atgtgctctt ttggtttgcg
      181 tcgtcggcgc cgacgggctt accgagggta ctctcatccg acaccacgtc gaggatgtcg
      241 tcggcgatct ggaacgccag ccccaaaggc tcggcgtagg cggcgaaccg aacgacatcg
      301 tcgagaggca cgccggccgc gatgagtcca caccgagcgg ccgcacggat cagcgaaccc
      361 gtcttgcgcg cgtggagcgc ccgcagcgcc tcgaaactga tgtggcgcgc ttcggcatcc
      421 aggtcctcag cctggccctc aacgatgccc gcacggcaca ccgcctcagc cagctcgcgc
      481 accaggcgca gcgtccgcgc gctctcgacg ccggcactct cgcccgcctc ggccacgagc
      541 gcgaacggga aacagagcag ccagtcgccg gcgagaacgg ccagcgactc ggcgaaagct
      601 cgatggcacg acggacggcc ccgccgcagg tcggagtcgt ccatgcacgg cagatcgtca
      661 tggatcagcg aggagacatg gatcagttcg agggcggcgg cggcgggcag agccgcctct
      721 gtcaagccgc cgaacgcctc gcaactcgcg atgcacagcg ccggacgaaa ccgcttgccg
      781 ccggcgaaga tcgagtaccg tactgcctcg tacagccgac cgtgggcgtg ggacgccgcc
      841 gggaggaccg tgtcgaggta ggagtccacc cgggctcccc gctcctcgag ataggtcgtg
      901 atatcgatca acgcaatcgg ggggcaatgg ccgccaccgg gcgccccccg catccccctc
      961 gttagccagg cggcgtctcg gcgaagggcg cctgactcag gtatcgctct cccgtgtcgc
     1021 agagaatcgt cacgatgagc ttgccggcgt tctccggcct ccgggcgacg ccgaccgcgg
     1081 cgaagacatt ggcccccgcc gatatgcccg ccaggatgcc ctcttccttg gctagccttc
     1141 gggccatggc catggcgtca tcgctggcaa cggtgataat ctcatcaacg agatccgtcc
     1201 gcagaacggc cggaacgaaa ccggcgccga tgccctggat cttgtggggt cccggcttcc
     1261 cgccggaaag caccggcgag gccagcggct cgaccgcgat ggcgcggaaa cccggccggc
     1321 gcgccttgat cagctcggcg caaccggtga gcgtaccccc ggtgcccaca ccggccacca
     1381 ggatgtccgc ctggccctcc gtgtcctccc agatctcctc ggccgtggtg gcgcgatgca
     1441 cccccgggtt cgccgagttc tcgaactgca tcgggatgaa cgagcccgga atctgtgcgg
     1501 ccagctcctg agcccgggcg atcgccccac gcatgccctc cgctccgggc gtgagttcca
     1561 gctcggcgcc gagcgccctg agcagcgcac gacgctcgag gctcatgctg tcgggcatgg
     1621 tcaggatgca gcgatagccg cgaaccgccg cgacccaggc cagcgcgatc cccgtgttgc
     1681 cactggtcgg ctcgatgatc accgaccccg ggccgatccg gccttcgcct tcggccgcct
     1741 cgatcatcga caggccaatg cggtccttca cgctcgagag cggattcatg aactcgcact
     1801 tgcccaccac cgtgccgacg cacccctcgg tcacccggtt gaggcgcacc aaaggcgtgt
     1861 gccccactag ctccgtcatg tcccccgcaa tgcgtcccac ggtagagcgc ccctctcgat
     1921 ggcgccggca atggagtcga ccggctg
//
LOCUS       DAOSPL010001001         1947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_58723_length_1947_cov_3.336152, whole genome shotgun sequence.
ACCESSION   DAOSPL010001001 DAOSPL010000000
VERSION     DAOSPL010001001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58723_length_1947_cov_3.336152"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1764
                     /locus_tag="PLQ54_16155"
     CDS             <1..1764
                     /locus_tag="PLQ54_16155"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOS94853.1"
                     /translation="PIEEALPVDTDVKQKKHFPSTTVVIAIDQSGSMSAMEDGKQKIE
                     MANEAACLVTDLLTPRDEIAVLAVDTMAKEVVELTRVEDPDGVKAKIRTLRGGGGGIY
                     CYTALSKAFEVARASEGKIRHVILLADGNDSEQQEGCLELVRQMAAERVTVTTVSIGA
                     GIHSDFLRDVAHWGKGRFYIANKMAELPRIYTKETFLVARSIVIEEPFRVQVTDPYDE
                     ASRGVDWAAAPPLLGYVGTTAKGRAHTPLLTHKADPLMAHWQYGLGRSLAFTSDATNR
                     WAARWLEWEQFGKLWAQWIRWTLKPSDPAAFETSVTIERGRGTITVDAVGDNHEYLNF
                     MESRARLLPPDLEGKTLELEQTAPGRYQATFEARGTGVYMINVTQKDGQGNSASQTTG
                     AVVPYPDEYRDLKPDTYLLSRLADTTHGKMLDLKDTASPFRRDRRAARTPVDIWWQLL
                     LWAVSLFILDVAVRRVAIERQQVEAVWARIAAVRHRARSRGEVQPHATLGRLKGVKQA
                     GRASGAAATAPPIAATPTPSEALSETAALQRQLDEAVRRAQPPVTPGAAESAPDSTEP
                     PDAPSGDTMGRLLAAKRRARR"
BASE COUNT          343 a          658 c          664 g          282 t
ORIGIN      
        1 cccatcgagg aggcgctgcc ggtcgacacc gacgtgaagc agaagaagca cttcccctcc
       61 accaccgtag tcatcgccat cgaccagtcg gggagcatgt ccgccatgga ggacgggaag
      121 cagaagatcg agatggccaa cgaggcggcc tgtctggtga ccgatctcct gactccccga
      181 gacgagatcg ccgtcctggc cgttgacacc atggccaagg aggtggtcga actcacccgg
      241 gtggaggacc cggatggcgt caaggccaag atccggaccc tgcggggcgg cggcggcggc
      301 atctactgct acacggcgtt gtcgaaggcg ttcgaggtcg cccgagcgtc ggagggcaag
      361 atccgccacg tgatcctgct cgccgacggc aacgattccg agcagcagga gggctgtcta
      421 gagctggtca ggcagatggc tgccgagagg gtcaccgtta ccaccgtgtc catcggcgcc
      481 ggcatccaca gtgatttcct gcgcgacgtc gcccactggg gcaaaggccg gttctacatc
      541 gccaacaaga tggccgagct cccgcgcatc tacaccaagg agacgtttct tgtggcccgg
      601 tccatcgtga tcgaggagcc gttcagggtc caggtgaccg atccgtacga cgaggcgtcc
      661 cgcggggtcg actgggccgc ggctcctccc ctccttgggt acgtgggcac gacggccaag
      721 ggccgggccc acacgccgct gctgacccac aaggccgacc cgctcatggc ccactggcag
      781 tacggtctgg gacgttcgct cgcattcacg tccgacgcca ccaaccgctg ggccgcgcgg
      841 tggctcgagt gggagcagtt cgggaagctg tgggcccaat ggatccgctg gaccctcaaa
      901 cccagcgatc cggccgcgtt cgaaacctcc gtgaccatcg agcggggacg ggggaccatc
      961 accgtcgacg ccgtgggcga taaccacgag tacctgaact tcatggagag ccgggcgcgg
     1021 ctgctcccgc cagatctcga aggcaagacg ctcgagctgg agcaaacggc cccgggccgc
     1081 taccaggcga cgtttgaggc caggggcacg ggtgtctata tgatcaacgt gacccagaaa
     1141 gacggccaag ggaattctgc ttcgcagacc accggcgcgg tcgtgcccta cccggacgag
     1201 taccgggacc tgaagcccga cacgtacttg ctgtcgcggc tcgccgacac gactcacggc
     1261 aagatgctag acctgaagga taccgcatcg ccgttccgca gggaccgccg agctgcgcgc
     1321 accccggtgg acatctggtg gcagctcctc ctctgggccg tgagcctgtt cattctcgat
     1381 gtggcagttc gccgggtcgc catcgagcgg cagcaggttg aggcggtctg ggccaggatc
     1441 gcggccgttc ggcaccgggc tcgctctcgt ggcgaggtgc agcctcacgc gactctcggc
     1501 cggctcaagg gcgtgaagca ggccggtcgc gcttccgggg cggccgccac agcgccaccg
     1561 atcgcggcga cgccgacgcc atccgaggcc ctgtcggaga cggcggcgct ccagcggcag
     1621 ctcgacgaag cggtgcgacg cgcccagccg cctgtcacac ccggcgccgc tgagtccgcg
     1681 ccagactcca ccgagccgcc agatgccccg tcgggcgaca ccatgggccg gctgctggcc
     1741 gccaagcgcc gagcgaggag gtaggcaccg ggcactggcg agcacgcgtc ggcgaaaggg
     1801 gatcacagca tgccttccag gaagcgcgcg gtaatgatcg gcaccggtgg attcggcggc
     1861 tggtggctcc ataactgcct tccccggttc gctggtgagc tcgagatcgc ggcgatcgcc
     1921 gacgtgaacg ccgaggcccg gtccgcg
//
LOCUS       DAOSPL010001002         1945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_58797_length_1945_cov_3.346032, whole genome shotgun sequence.
ACCESSION   DAOSPL010001002 DAOSPL010000000
VERSION     DAOSPL010001002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58797_length_1945_cov_3.346032"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PLQ54_16160"
     CDS             complement(<1..414)
                     /locus_tag="PLQ54_16160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOS94854.1"
                     /translation="MALTAGLDVGSTTTKVVLVEGDRMVAYDLAPTGASCRRAAERLL
                     ASALARSGYAPSDVHYMVSTGYGRRLVAATDQTVSELTANAVGARFLANGSTVRTIID
                     IGGQDSKVIVLDEQGQMTNFAMNDKCAAGTGRFLEQ"
     gene            586..1260
                     /locus_tag="PLQ54_16165"
     CDS             586..1260
                     /locus_tag="PLQ54_16165"
                     /inference="COORDINATES: protein motif:HMM:NF017530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
                     /protein_id="HOS94855.1"
                     /translation="MLSLPEEFLLLALDDQKGSFAAFTDYGLAGAVLVELLLRDAITV
                     GPKGEVLAGSSPQGDAALDAALAAIGQLKKPKKLQEWVGSLPGRCKTLKPAVLDGLVA
                     QGVLRREDRRFLRVFAYTRYPEADGAPEDAIVGRLRRVLLDGQEPDGRTAALIGLLHG
                     CQMDASVLRNRAERKTAKPILDRITKGDIVSSAVSSAVAAAIAAACSAAMTCCIAASV
                     AASSSS"
     gene            complement(1343..>1945)
                     /locus_tag="PLQ54_16170"
     CDS             complement(1343..>1945)
                     /locus_tag="PLQ54_16170"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOS94856.1"
                     /translation="GPLQVRPNELQRRDGPAESFVSLNANPVTSGWAITALELIGANL
                     QRAVADGTDLEARAAMQIASALGGAALNAGVGCAHMLAHPLGATFGIPHGVAIAAVLP
                     AVFEANRIDAESQYARIARALDPECSGLPDAEAATRAGSALRGLLERIGLCERLGGRG
                     VTAADFGRVYEGVLRSMGHIKTNPRPATRELLESIFAASL"
BASE COUNT          293 a          719 c          623 g          310 t
ORIGIN      
        1 ctgctccagg aaccgccccg tgcccgcggc gcacttgtcg ttcatggcga agttggtcat
       61 ctggccctgc tcatccagca cgatgacctt gctgtcttgg ccgccgatgt cgatgatcgt
      121 ccggacggtg gagccgttgg ccaggaagcg ggcccccacc gcgttggccg tcagctcgct
      181 gaccgtctgg tccgtggccg ccacaagccg ccgcccatac ccggtgctca ccatgtagtg
      241 cacatccgaa ggcgcatagc cgctgcgtgc cagggccgag gccagtagcc gctccgccgc
      301 cctccggcaa ctggcgcccg tcggcgccag gtcatacgcc accatacggt cgccctcgac
      361 cagaaccacc ttggtggtcg tcgatcccac atcaagtccc gcagtcagcg ccatgcctct
      421 acacactcgc tccagacgtg tgggctagtg taccccatcc caccgcagtc agcgagcggc
      481 gatgcgcggc agttccagtg gataggcgca accgccgcct gtgccgaacg tcctgatgcc
      541 tgacactgcg cacccagacc acctgagatg tgaggagtgg actccgtgct gtcgctgcct
      601 gaggagttcc tgctgctggc gctggatgac cagaaggggt cgttcgctgc ctttaccgac
      661 tatggcctgg ccggggcagt gctggtcgag ctgctgctgc gtgacgccat caccgtgggc
      721 cccaagggcg aggtcctggc tggtagttcc ccccagggcg acgcggcgct cgacgccgcg
      781 ctggcagcca tcggccaact gaagaagccc aagaagctcc aggagtgggt cggctctcta
      841 cccggacgct gcaagacgct caagcccgcg gtgctcgatg gcctcgtggc gcagggggtt
      901 ctgcgtcggg aggaccgccg ctttctccgc gtgtttgcct atacgcgcta ccctgaggcg
      961 gacggggcgc ccgaggacgc gatcgtcggc cgcttgcgcc gtgtgctgct cgatgggcag
     1021 gagcccgacg gtcggaccgc cgccctcatt ggcctgctcc acggatgtca gatggacgcg
     1081 tccgtgctcc gcaaccgtgc cgaacgcaag accgccaagc cgatactgga ccgcatcacc
     1141 aagggcgaca tcgtgagctc ggcggtgagc agcgcggtgg ccgcggccat cgccgccgcc
     1201 tgctcggctg cgatgacatg ctgcatcgcg gcctcggtcg ccgcgtcatc cagcagctga
     1261 ctgcctggcc tcgcacacgc gccggacggc ctcctcaggc tcctgacgcg gggttgccgc
     1321 catctgcctt ctgccacctg tctcacagcg acgccgcgaa gatcgactcc agcagctcgc
     1381 gcgtcgccgg gcgtgggttc gtcttgatgt gccccatcga tctcagcacg ccctcataga
     1441 ctcggccgaa gtcagctgcc gtcaccccgc gcccccccag ccgctcgcag agcccgattc
     1501 gctccaggag accccggagc gcactgcccg cgcgcgtcgc cgcctcggcg tccggcagcc
     1561 cgctgcactc cggatccaac gcccgagcga tccgcgcgta ctggctctca gcgtcaatgc
     1621 ggttggcctc aaagacggca ggaagcacgg cggcgatggc cacgccgtgg ggaatgccga
     1681 acgtcgcgcc caacgggtgg gccaacatgt gtgcacaccc cacgcccgcg ttgagggcgg
     1741 cgccccccag cgcgctagcg atctgcatgg ccgcccgcgc ctccaggtcc gtcccgtccg
     1801 ccacggccct ctgcaggttc gccccaatga gctccagcgc cgtgatggcc cagccggacg
     1861 tgaccgggtt cgcgttcagc gacacaaacg actcggctgg gccatcacgg cgctggagct
     1921 cattggggcg aacctgcaga gggcc
//
LOCUS       DAOSPL010001003         1943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_58892_length_1943_cov_3.512182, whole genome shotgun sequence.
ACCESSION   DAOSPL010001003 DAOSPL010000000
VERSION     DAOSPL010001003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58892_length_1943_cov_3.512182"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="PLQ54_16175"
     CDS             complement(<1..1530)
                     /locus_tag="PLQ54_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94857.1"
                     /translation="MARYGSMRAVLAPVAVLLMAACAEALPSLNVIRVDSLSRVYPDR
                     TPTPAAYAQPVSVPRGASVSFVFAAMMTPGQICALSVSPPRQEDGTGIVAQSALYEVL
                     PVHIEANTNVPNRDTPASPPPPDWMQALVRPAPFDVAEVLVPADRLDLSDGLWHAALV
                     DVFVAPDCPPGRYTGHMALRANHVARELPFTLDVRATCLEDYPALDYLLEIRPDPDLL
                     TTRAAPDWWSEEHWRLLEITGRRLRAAGQTHMLTPLVNYEQPLIRVARDENGQFAFDF
                     ARFDRWMRLFLGLGFRKMVGQPIALLPAGEAPDGTYVIDKLTRKRERLCDPAQPEALE
                     RWLSFIPVFYKALKAHLDAHGWTGVYIQQQLGEPKDAGVYGLLSEMLRSNMPGVPAMD
                     AINESPAALSPLVDVQVFAVTVLAAEQALAADRHRQGLANWLFTGAVPLPPFPNRHLD
                     EPLANSRLYPWMAFGLGAQGYVCYGGNAYRGANPYRTSIGPRPDSPRGASFGPGEDWM
                     LY"
     gene            1740..>1943
                     /locus_tag="PLQ54_16180"
     CDS             1740..>1943
                     /locus_tag="PLQ54_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94858.1"
                     /translation="MKALPWKPIAIAAAVVVVVGGGATSLFLIQPWKKATRPAAGADA
                     AKPTQPAPKLVAEVGKVPITQEAF"
BASE COUNT          351 a          639 c          663 g          290 t
ORIGIN      
        1 gtacaacatc cagtcctcgc cggggccgaa gctcgcaccg cgcggactgt cgggccgcgg
       61 cccgatcgag gtccggtagg ggttggcccc ccggtacgcg ttgcccccgt agcacacgta
      121 gccctgcgcg cccaggccaa acgccatcca ggggtagagg cgggagttgg ccagcggctc
      181 atcgaggtgg cggttcggaa acgggggcag tgggaccgcc cccgtgaaca gccagtttgc
      241 aaggccctga cgatgacgat cggccgccag ggcctgctcc gcggccaaga cagtcacggc
      301 aaagacctgc acgtccacca gcggcgacaa agccgccggg gactcgttga tggcatccat
      361 ggccggcact ccgggcatgt tggaccgaag catctcagac aggagcccat ataccccagc
      421 atctttgggc tcgcccaact gctgctggat gtacaccccg gtccatccat gggcgtcgag
      481 atgtgccttg agggccttgt agaagacggg gatgaacgag agccaccgct cgagcgcctc
      541 gggctgggcg gggtcacata gccgttcccg cttccgggtc agcttgtcga tcacgtaggt
      601 cccatcgggc gcctcgcccg ccggtaggag tgcgatcggc tgacccacca tctttcggaa
      661 gccaaggccc aggaacaggc gcatccagcg gtcgaagcgg gcgaagtcga aggcaaactg
      721 gccattctcg tcgcgcgcca cacgaatgag gggctgctcg tagttgacga gcggcgtcaa
      781 catgtgggtc tgtccagccg cgcggagccg gcgccccgtg atctcgagaa gtctccagtg
      841 ttcctcgctc caccagtcag gcgccgccct cgtcgtcaga aggtcagggt ctggtcggat
      901 ctcgagcagg tagtcgagag ccgggtagtc ctccaggcag gtggcgcgga catccaatgt
      961 gaagggtagc tcacgcgcta catggttcgc gcgcagcgcc atgtgcccgg tgtatcgtcc
     1021 cggcgggcag tcgggggcca cgaacacatc aacgagagcg gcgtgccaca gcccgtccga
     1081 caggtcgaga cggtcggcgg gaaccagtac ctcggcaaca tcgaagggag ccggccgcac
     1141 gagcgcttgc atccaatcgg ggggcggtgg ggacgccggc gtgtcccggt tcggcacgtt
     1201 cgtgttggcc tcgatgtgga caggcaagac ctcatacaga gcagattggg ccacgattcc
     1261 cgtgccgtct tcctgtctgg ggggagatac cgatagcgca cagatctggc cgggggtcat
     1321 catcgccgcg aacacgaacg acaccgaggc gccacgcggc acgctcaccg gctgagcata
     1381 tgccgcgggc gtgggggtcc ggtccgggta cacgcggctg aggctatcga cccgtatgac
     1441 gttgagggat gggagcgcct cggcacacgc cgccatcaag agcacggcga cgggagctaa
     1501 caccgctcgc atggagccat accgcgccat gcggcagcgg cctcctcaga tgcccggtgt
     1561 ccgattacgg cggcccggac cgtcatcgaa agaaggactt cgcgccccaa agccaaacgt
     1621 cctacccgag acccgcgtgg agcaagcacc gaaccttgcg cgggtactgc cgttggtacc
     1681 acgaagcccg gcaccggcgc cccaagcggc gctggggccg accgactgga ggcgtcgtcg
     1741 tgaaggctct gccctggaaa cccatcgcca ttgcggcggc cgtcgtcgtg gtcgtgggtg
     1801 gcggcgccac gtccctgttc ctgatccagc cgtggaagaa ggcgacccgc cctgcggccg
     1861 gtgcagacgc cgcaaagcct actcagccag cgcccaagct cgtggccgag gtcggcaagg
     1921 tgccgattac gcaggaggct ttc
//
LOCUS       DAOSPL010001004         1943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_58907_length_1943_cov_2.556674, whole genome shotgun sequence.
ACCESSION   DAOSPL010001004 DAOSPL010000000
VERSION     DAOSPL010001004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58907_length_1943_cov_2.556674"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1010)
                     /locus_tag="PLQ54_16185"
     CDS             complement(12..1010)
                     /locus_tag="PLQ54_16185"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOS94859.1"
                     /translation="MSGSCCGIAPDQLYVGLDVGGTGLKGLLCTGAGEVKARASSPTE
                     AAQGYEAVMSRILDLTEGLVAQASQPVAGIGLALAGNLRSAQGTCVFSPNLPGWRDVP
                     VAGPILERFPTRFRMGNDANCAALAEHRFGAGRGTTHMAMLTLGTGVGGGLIIDRRLQ
                     VGPREGMAEIGHMIVDPNGPECGCGNHGCLEAFCGAAGISRAARRMLQTGRPSMMLDL
                     AQGDILNVTPLVVATAASQGDALACEVLATIGNVLGTGLTSICMLVDPDKIILGGQIA
                     QAGEPLLEPARRTIRARARMVPFDPQNVVLAQLGGEAGSLGAVALFDDEGIPELTP"
     gene            complement(1144..1386)
                     /gene="rpsR"
                     /locus_tag="PLQ54_16190"
     CDS             complement(1144..1386)
                     /gene="rpsR"
                     /locus_tag="PLQ54_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOS94860.1"
                     /translation="MPPRRERRQRRPRRKVCSFCADKVDYIDYKDVPLLRRYMSDRGK
                     IMPRRSSGTCAKHQRRLTVAIKRAREIALVPFVAGE"
     gene            complement(1444..1917)
                     /locus_tag="PLQ54_16195"
     CDS             complement(1444..1917)
                     /locus_tag="PLQ54_16195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOS94861.1"
                     /translation="MINRVVLVGRLGNDPELRVTASGISVCSLRLAVRRPRAQEGQPD
                     TDWIDVVAWRQNAEFVANYLKKGYLVGVDGRLQVREWENQAGEKRRSVEVVADSIQNL
                     QPKERSGDEPPPRRGAEAPGPARGTERAAAPSAIEEDMREAFGDDDGGFDPFADA"
BASE COUNT          313 a          731 c          582 g          317 t
ORIGIN      
        1 ctctgcccgg cctagggggt gagctctggg atcccctcat catcaaacag cgccacagca
       61 cccaggctgc cggcttcgcc gcccagctga gccagaacca cgttctgagg gtcaaacggt
      121 accatgcgcg cacgggcccg aatcgtacgg cgagccggct ccagaagcgg ttctcctgcc
      181 tgcgcgatct ggccgcccag gatgatcttg tcgggatcca ccaacatgca gatgctcgtc
      241 agcccggtgc ccagtacgtt gccgatcgtg gcaagcacct cgcacgccaa ggcgtcgccc
      301 tgggacgccg ccgtggcgac aaccaagggg gtcacgttca gaatgtcgcc ctgggccagg
      361 tcgagcatca tgctggggcg ccctgtctgc agcattcggc gcgccgcccg cgagatcccg
      421 gcggcgccgc agaatgcctc cagacaccca tgattgccac acccgcactc gggaccgttc
      481 gggtcaacaa tcatgtgccc aatctctgcc atgccctcgc gcggtcccac ctgcaaccgc
      541 ctgtcgatga tcaggccacc gcccacgccc gtgcccaagg tcagcatggc catgtgggtg
      601 gtcccgcggc ccgcaccgaa gcggtgttcc gccagcgcgg cgcagttggc gtcgttgccc
      661 atacggaatc gcgtcgggaa acgttcgagg atcgggcccg cgaccggcac gtctctccag
      721 cccgggaggt tgggcgagaa cacgcacgtc ccctgagcgg accgcaggtt gcccgccagc
      781 gccagaccga tgccggccac cggctggctc gcctgggcga cgagaccctc cgtcaggtcg
      841 aggatccggc tcatgacggc ctcatagccc tgcgccgcct cggtggggct cgatgcccgc
      901 gccttcactt ccccggcgcc ggtacacagg agccccttca gccccgtccc accgacgtcc
      961 aggccaacgt agagctggtc tggagcgatt ccgcaacagg aacccgacat ccgctacacc
     1021 gccatcccca aagcccgaac ttgctcggaa ctggccccgc aaacaccaac ggccgcaggc
     1081 caaagggctc ggcctgcggc gtgcggcctt cgtcgggacc ctggaccaac tggtacgggt
     1141 tgcctactcc cctgcgacga acggaaccag ggcgatctca cgggcacgct tgatggccac
     1201 cgtcagacgc cgctgatgtt tggcgcaggt accactgctg cggcgaggca tgatcttgcc
     1261 tcggtccgac atgtatcggc ggagcagcgg cacgtccttg tagtcgatgt agtccacctt
     1321 gtcggcgcag aacgaacaca ccttgcgccg gggcctgcgt tgccttctct ctctgcgtgg
     1381 aggcaccttg tgactctcca tttccgatct cccaagccgt tcaccggccc ggaatgttcc
     1441 cgcctaggca tcggcgaagg ggtcgaaacc cccgtcgtcg tcgccgaacg cctcgcgcat
     1501 gtcctcttcg atggcggagg gcgccgcggc tcgctcggtg ccccgcgccg gaccgggggc
     1561 ctccgcgccg cgccgtggcg gcggctcgtc gccgctgcgc tccttcggct gcagattctg
     1621 gatgctgtcc gcaaccactt caaccgaccg gcgcttctcc cccgcctggt tctcccactc
     1681 gcgcacctgc agtctgccgt cgacgccgac caagtagccc ttcttgaggt agttcgccac
     1741 gaactcagcg ttctgccgcc aagccaccac atcgatccag tccgtgtccg gctgcccctc
     1801 ctgggcacgc ggcctgcgaa ccgccagccg cagcgaacag accgagatgc cgcttgccgt
     1861 gacccgaagc tctgggtcat tcccgagacg accgaccagc accacgcggt tgatcatagc
     1921 gcctcactcc ccgtgaatgg ctc
//
LOCUS       DAOSPL010001005         1943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_58917_length_1943_cov_1.995233, whole genome shotgun sequence.
ACCESSION   DAOSPL010001005 DAOSPL010000000
VERSION     DAOSPL010001005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58917_length_1943_cov_1.995233"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..>1943
                     /gene="selB"
                     /locus_tag="PLQ54_16200"
     CDS             207..>1943
                     /gene="selB"
                     /locus_tag="PLQ54_16200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOS94862.1"
                     /translation="MRHAVIGTAGHVDHGKTTLIRALTGVNTDRLRQEQERGLTIELG
                     FAPLTLPSGRTAGVVDVPGHERFIKNMLAGATGVDVALLVVAADEGVMPQTDEHLDIL
                     NLLGVRHGVVAVTKADLVEAEWLELVAEDVRDRLADTLLAGVAIVPVSAVTGQGLDEL
                     LRELDLALDRAASASAAERWSCTRLPIDRVFAISGHGTVITGTLIGGSIKPGDRLELL
                     PAGKSVRVRQVQVHDKTVEAAERGQRVALNLAGLERDEIERGMVLAAPAMLEPTRLFD
                     AELTLLRSATPVEHGDCMHLHMGTAEATCRVRVIGGDAAIPGEITFVQLELAECEGVA
                     ARGDRFIIRTYSPMATAGGGRILAVARGRMRRFRPEIVREFETLSGGDDAELATLFVH
                     RALREHARPATAADVARAMFREEASVRALLERGVAAGAIVELGGLGSGGAGAGGAGDG
                     DQRGYLTQGDWADVEERMHDVLADFHAREPLRAGMAREELRTRALPGWDSKWFTLAMS
                     RAAADGTVHEQRALAALPSHRVTLSPEQQEWARRIEDAFASSLMSPPERPDVMAGGAP
                     QAVLDYLIDTGVV"
BASE COUNT          321 a          552 c          728 g          342 t
ORIGIN      
        1 ggccattccc cggtcgtggc ccgagtggct gacggttcag tccgtctgca cctcaggacc
       61 atattcgacc gcgatttcgg ccggctggcc gcggcgacgg cgtcggccat agcgtctgca
      121 catgcgtgag ccgcaggatc ggccggcgga ggtcgcaccg ggcgcgaaac atgaagtcct
      181 agaaaaccga ggggaaatgt agtctcatga gacatgcggt gatcggtacg gcaggccatg
      241 tggaccacgg caagaccact ctgatccgtg cgcttacggg cgtcaacact gaccggcttc
      301 gccaggagca ggaacgggga ctcacgattg agctggggtt tgctcccctc acactgccct
      361 cgggccgtac tgccggcgtg gtggacgtgc ccggccatga gcggttcatc aagaacatgc
      421 tggccggggc gaccggagtg gatgtggccc ttttggtggt ggcggccgat gagggtgtta
      481 tgccccagac agatgagcat ctcgacattc tgaacctcct gggcgtccgg catggtgtgg
      541 ttgcggtgac caaagccgac ttggtcgagg cagaatggct ggagcttgtg gcggaagatg
      601 tacgtgaccg tctggccgat acgcttcttg ccggggtggc aatagtgccg gtgtcggcag
      661 tgaccgggca gggtttggac gaactgctcc gagagctgga cctggctctg gaccgggcag
      721 cgtcggcttc cgcggccgaa cgctggagct gcacccgtct tcccatagat cgtgtattcg
      781 cgatatcggg ccatggcact gttatcaccg gtactctcat tgggggttcg atcaagcccg
      841 gcgaccgcct ggaactgctt cctgccggta agtcggtgag ggtgaggcag gtgcaggtgc
      901 atgataagac ggtagaagcg gccgagcgcg ggcagcgggt agcgctcaac ctggccgggc
      961 tggaacgcga cgagatcgag cgaggtatgg tgctggcagc gccggcgatg ctggagccga
     1021 cgcggctgtt cgacgccgag ttgacgctgc ttcggtcggc gacgccggtg gagcatggcg
     1081 actgcatgca ccttcacatg ggcaccgccg aagctacgtg cagggtgagg gtgataggcg
     1141 gcgacgcggc tattcctggg gagatcacct ttgtgcagct ggaacttgca gagtgcgaag
     1201 gcgttgcggc gcggggcgac cggttcatca tccggaccta ttcgcccatg gccacagccg
     1261 ggggcggacg gatcctggcc gtcgcgcgag gccggatgcg gcgatttcgc ccggagatcg
     1321 tgcgggagtt cgaaaccttg tccggagggg acgatgcgga actggcaaca ctgttcgtgc
     1381 atcgggctct ccgcgagcat gcccgcccgg cgactgccgc ggatgtggcg cgggccatgt
     1441 tccgtgaaga ggcgagcgtg cgcgcgctgc tcgaacgagg ggtagccgcg ggcgcgattg
     1501 tggagctggg cggcttgggc agcggcggag ccggggcggg cggcgcaggc gatggtgacc
     1561 agcgaggcta cttgacgcag ggcgactggg cggacgtcga agagcgaatg cacgatgtgc
     1621 tggcagactt tcatgcccgc gaaccgctcc gggccgggat ggccagggag gaattgcgga
     1681 cccgggcgct tcccggctgg gactccaagt ggttcactct ggccatgtct agggccgcgg
     1741 cggacggcac agtgcacgaa cagcgcgctc tggcggcgtt gccgtctcat cgagtgaccc
     1801 tcagccccga gcagcaggag tgggcgcgcc gaatagagga cgcgtttgct tcctccctta
     1861 tgtcgccgcc ggagaggccc gatgtgatgg caggcggggc tcctcaggct gtgctggatt
     1921 acttgatcga cacgggcgtg gtg
//
LOCUS       DAOSPL010001006         1942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_58937_length_1942_cov_3.408055, whole genome shotgun sequence.
ACCESSION   DAOSPL010001006 DAOSPL010000000
VERSION     DAOSPL010001006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58937_length_1942_cov_3.408055"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PLQ54_16205"
     CDS             complement(<1..429)
                     /locus_tag="PLQ54_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94863.1"
                     /translation="MAYCYVPVYRAATGTQTRVGSGAARQGGGGVSEHSADSQGASAD
                     QAAAGSRAGSGGHRHTVDIRVVTPAWLGATYVDIRMGRDRRTVPRAHLVARSVRRMNR
                     FVFAMGAQMLAAWLLWVGLGFYWVTQSACGGGAVLSTPSDI"
     gene            complement(500..1492)
                     /locus_tag="PLQ54_16210"
     CDS             complement(500..1492)
                     /locus_tag="PLQ54_16210"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOS94864.1"
                     /translation="MSDPQAMRNITYAQAIRTALREEMKRDERVVLWGEDIGVYGGVY
                     GATTGLQREFGTERVRDTPISEMAIVGMGVGAALLGLRPVVEIMYEDFLAHAADGVVN
                     QAAKWGYMSGGQYRVPLVVRSPGGGGSGYAAQHSQNLEAWFAHIPGLIVVATCMPRDA
                     RGLLKAAIRDDNPVVFLEHKGHYTYKGDVPRDEEVLPLGKADIKRPGRDVTIVAWSKA
                     LVAALSAADELASEGIDAEVIDPRTLLPLDMDTILASVGRTRHLVVVSESHAYCGVSA
                     EISAQVCERAFGELKRPPKRVGSHFVPLPYSEPMESYVIPDAARVAEAVRGLLA"
     gene            complement(1521..>1942)
                     /locus_tag="PLQ54_16215"
     CDS             complement(1521..>1942)
                     /locus_tag="PLQ54_16215"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOS94865.1"
                     /translation="ALGYGIEGHRIDGMNVLDVYDISMAAIEKARREGRPSFLVFDTY
                     RFVGHHTADSEHYRPKDEVLQEFRAHDPIHFVERSVADDCIVDMTELVRIRDEVRAEV
                     DAAFARALGAPWPSPEDALGGAYVETSASFTCTGLPG"
BASE COUNT          331 a          671 c          630 g          310 t
ORIGIN      
        1 gatgtccgac ggagtgctga gtacggctcc gccgccacag gcgctctggg tgacccagta
       61 gaagcccaga cctacccaga ggagccatgc cgccaacatt tgcgcgccca tggcgaagac
      121 gaaccggttc atccgacgaa ccgagcgggc gaccaagtgc gcgcgcggaa cagtgcggcg
      181 gtcgcggccc attcgtatgt cgacataggt cgcgcccagc caggccgggg tgacgacccg
      241 aatgtcgacc gtatgccgat ggcctccgga ccccgctctc gacccggctg ctgcctggtc
      301 agccgaagct ccctgcgaat cagcgctgtg ttccgacacc ccaccgcccc cttggcgggc
      361 ggccccggac ccaacacgcg tctgcgtgcc cgtggccgct cggtacaccg gtacatagca
      421 gtatgccatc cccggaacct caatgggtgc gccgcggtga agatcggcgg ggaagaggat
      481 tccgccgagc tgctggcgct caggccagca ggccccgaac cgcctcagct acccgggccg
      541 cgtcggggat cacgtacgac tccatcggct cagagtaggg cagtggcacg aagtgcgacc
      601 ccacgcgttt gggtggcctc ttcagttcgc cgaacgcccg ctcgcacacc tgggcgctga
      661 tctcggcgct gacaccgcag taggcatgag attcggaaac caccaccagg tggcgcgtcc
      721 ggcccaccga cgccaggatg gtgtccatgt ccagtggcag gagcgtccgg ggatcgatga
      781 cctcggcgtc gatgccctca gaggccaact cgtcggcggc cgagagtgcc gcgaccagcg
      841 ccttggacca ggccacgatg gtcacatcgc gccccgggcg cttgatgtcg gccttaccca
      901 gcgggagcac ctcttcgtct cgcggaacat cgcccttgta ggtgtagtgg cccttgtgct
      961 cgaggaatac caccgggttg tcgtctcgga tcgcagcttt gaggagtccg cgggcatcgc
     1021 gaggcatgca cgtggcgacc acgatgagcc ccggaatgtg cgcgaaccag gcttcaagat
     1081 tctgcgagtg ctgggccgca tagccggacc cgccgccccc ggggctccgc acaaccagtg
     1141 gcacgcggta ctgcccaccg ctcatgtagc cccacttggc ggcttggttc acgacgccgt
     1201 cagcggcatg ggcgaggaag tcctcgtaca tgatctcaac cacagggcgg aggcccagca
     1261 gcgccgcgcc gacgcccatg cccacgatgg ccatctccga gatgggcgtg tcgcgcactc
     1321 gctcggtccc aaactcgcgc tggaggcccg tggtggctcc atagacgcca ccataaacgc
     1381 cgatgtcctc gccccacagg acgacgcgct cgtcgcgttt catctcctcg cgaagcgcgg
     1441 ttcggatggc ctgagcgtag gtgatgttgc gcatcgcttg ggggtcgctc atcccagtga
     1501 ctccttcggc gctcggttgg ctacccgggc aagccggtgc aggtgaatga ggcagaggtt
     1561 tcgacgtagg cgccgccaag tgcgtcctcg ggagacggcc agggcgcccc caaggctcgg
     1621 gcgaaggccg catcgacctc ggcgcgcact tcgtcgcgga tgcgcacgag ttccgtcata
     1681 tccacgatgc agtcatcggc caccgaccgc tcaacaaagt ggatcgggtc gtgggcccgg
     1741 aactcctgga gcacctcgtc cttgggcctg tagtgctccg agtcggccgt gtgatgcccc
     1801 acgaagcggt aggtgtcgaa gaccaggaac gaggggcgcc cctcgcgccg ggccttctcg
     1861 atggcagcca tggagatgtc gtacacgtcc agcacgttca tgccatcgat ccggtgcccc
     1921 tcgatgccat agcccagagc cc
//
LOCUS       DAOSPL010001007         1941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_58980_length_1941_cov_3.339343, whole genome shotgun sequence.
ACCESSION   DAOSPL010001007 DAOSPL010000000
VERSION     DAOSPL010001007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58980_length_1941_cov_3.339343"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="PLQ54_16220"
     CDS             complement(<1..1027)
                     /locus_tag="PLQ54_16220"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOS94866.1"
                     /translation="MTRRTRVLVVDDSAFMRKMIAELLGAEPDVEVVGSARNGEEGVA
                     LTQSLRPDVVTLDIEMPVMDGLTALRTIMAKCATPVVMLSSLTADGGDITMRALRYGA
                     ADFVHKPSGAISLDIAKVRDELVSKVRAAATVDVRRLAPSVLPVPAMPAAAPVSPEAP
                     RRAPTAGPADRLVAIGTSTGGPRALQAVVPFLPADLPAGVLIVQHMPPKFTKSLADRL
                     NADSQIEVREAQPGDTVTTGVALLAPGDYHMRVTADRRIELTQDPPLWGVRPAVDVML
                     DSAVRLFERRMLAVVMTGMGRDGRDGCVQVRQAGGRVVAEHEETCVVYGMPRAVVEAG
                     VADEVLPL"
     gene            complement(1024..>1941)
                     /locus_tag="PLQ54_16225"
     CDS             complement(1024..>1941)
                     /locus_tag="PLQ54_16225"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOS94867.1"
                     /translation="SRGGPTVQRMGTTSPTPDEIVQRVRDLPAMPLVVTQVLRATRDP
                     DSSVADVAQILGTDQGLTARVLRLANSALFGFPRRVATLTDAVVLLGFSTLRSLVLAG
                     TAFGLLNRAVSGYSLDKGALWRHSMGAGVAARLVCQRQRLRRLTEEAFVAGVLHDMGK
                     LVLDTYVREQFSQIIQRVEENREPFMAAERAILGYDHAQLGAMAAETWALPADLCEAI
                     RLHHEPPPWSEPRLFHVVHVADVIALSLGMGLGSDGLNYAVDSGAMETLGLSGADVEE
                     LISQMPDALTDIDTFLGEDGGAAGGGGAA"
BASE COUNT          329 a          704 c          631 g          277 t
ORIGIN      
        1 gcaggggaag cacctcgtcg gcgacccccg cctcgaccac cgcgcgcggc atcccgtaga
       61 ccacgcatgt ctcctcgtgt tcggcaacca cgcgtccgcc cgcttgccgg acctggacgc
      121 aaccgtcgcg tccgtctcgc cccatcccgg tcatcacaac ggccaacatc cggcgctcga
      181 acagccgcac cgccgagtcg agcatgacgt ccactgcagg ccgcacaccc cagagcggcg
      241 ggtcttgcgt gagctcaatc cgccgatcag ccgtcacccg catgtgatag tcgcccggcg
      301 ccagaagggc aacccccgtg gtgacggtgt cccccggctg ggcctcgcgc acttcgatct
      361 gcgaatccgc attgagccgg tcggccagcg acttggtgaa cttgggtggc atgtgctgca
      421 cgatcagaac gcctgccggc agatccgccg gcaggaacgg aacgacggcc tgcagtgccc
      481 gcggacctcc ggtggatgtc ccgatcgcca ccagccggtc ggccggaccg gccgttggag
      541 cccggcgcgg cgcctcggga ctcaccggag ccgccgccgg catggccggg accggcagca
      601 cggacggcgc cagtctccgc acatcgacgg tcgcggcagc gcgcaccttg ctgaccagct
      661 cgtcgcgcac cttggcgatg tcgagcgaga tcgcgcctga aggcttgtgc acgaagtccg
      721 ccgccccgta acgcagggcg cgcatggtga tgtcgccgcc gtcggccgtc aggctgctca
      781 gcatcaccac cggagtcgca cacttggcca tgatggtccg aagagccgtg agcccgtcca
      841 tgacgggcat ctcgatgtcc agggtgacca catcggggcg cagggactgg gtgagcgcga
      901 cgccctcctc gccattacgg gcggagccga cgacctcaac atcgggctca gcccccagca
      961 gctcggcgat catcttccgc atgaacgccg agtcatcgac aacgagaacg cgcgtccgcc
     1021 gggtcacgcg gcaccgcccc caccggcggc cccgccatcc tcgcccagga acgtatcgat
     1081 gtcagtcagg gcgtctggca tctgcgaaat cagttcctcc acatcggcgc ccgaaagccc
     1141 cagcgtctcc atggcgcccg aatcgacggc gtagttgagg ccatcgctcc cgagccccat
     1201 cccgagcgac agcgcgatca catccgccac atgcaccacg tggaacaggc ggggctcgga
     1261 ccatggcggc ggctcgtggt gtagtcggat ggcctcacac aagtctgcgg ggagggccca
     1321 ggtctcggcg gccatggcgc cgagttgagc gtggtcatag ccgaggatgg cacgctcggc
     1381 cgccatgaag ggctcgcggt tctcctcgac ccgctggatg atctggctga actgctcacg
     1441 gacataggtg tcgaggacca gcttgcccat gtcatgcagc acaccggcca caaaggcctc
     1501 ctcggtgagg cggcgcagcc gctggcgctg gcacacaagg cgagccgcca cgccggcacc
     1561 catggagtgg cgccacagcg cgcctttgtc gagcgagtac ccgctcacgg cacggttgag
     1621 gagcccgaag gcagttccgg cgaggaccag gctgcgcagg gtgctgaacc caagcaggac
     1681 taccgcgtcg gtcagtgtgg ccacacgacg cgggaagccg aagagcgccg agttcgccag
     1741 ccgcagcacc cgggcggtca gcccctggtc cgtgcccagt atctgagcga cgtcggccac
     1801 cgagctgtct ggatcgcgcg tggctcgcag cacctgggtc accacaagcg gcatcgcagg
     1861 caggtcacgg acgcgctgca cgatctcgtc tggagttggg ctcgtagtac ccatcctctg
     1921 cacggtcggg ccgcctctac t
//
LOCUS       DAOSPL010001008         1940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_59033_length_1940_cov_3.121485, whole genome shotgun sequence.
ACCESSION   DAOSPL010001008 DAOSPL010000000
VERSION     DAOSPL010001008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59033_length_1940_cov_3.121485"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..616)
                     /locus_tag="PLQ54_16230"
     CDS             complement(197..616)
                     /locus_tag="PLQ54_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94868.1"
                     /translation="MPEVRRYPVRADLRSLAAALESFFQRERFETQWFEQGEGYLVTA
                     KRMDTVRFAVGVGREATVTLMPSGEGTLVTVDRGALTSQAVGGVLGYALLGPIGIGAA
                     GYGAYKRHQLADEIFDVIERSLARSSGLASTDDLDMP"
     gene            complement(648..1664)
                     /locus_tag="PLQ54_16235"
     CDS             complement(648..1664)
                     /locus_tag="PLQ54_16235"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOS94869.1"
                     /translation="MSLCIPEAVALNLGGHSRRDFLKAIAGAAGAAVALRAGLWADAQ
                     TSSKVAIAHHSKVVTPDGERDTAVLTAMVDQSMAALTGKTDAVEAWRSLFEPTDVVGL
                     KVNCLAGFGLSSSVALVSVLTRRLQDAGVPASSIIVWERSDRELIQGGFEVNTDGPGV
                     RCFGTNGKVTAEMSQGQWKGEVSQILAEQITALINVPILKDHNLSGVALSLKNHYGSF
                     ARPGDFHADGCDPFIADLNALPLIRQKQRLIVCDATVGCYEGGPTMNPQFVFEPGAIL
                     MSTDPVALDYQGWQMIEAARQANGLPPLEAVGRHPKQIDSAAQRGLGTNDPASMEVAR
                     LELG"
BASE COUNT          301 a          651 c          660 g          328 t
ORIGIN      
        1 tcgcgcccag gcgatcatgg aggcggttgg gggccggtgg ccgggagtgt atgtgccaac
       61 gaagaccttc gtcatgtgct tcgggagcac gggagcctac cagaaggcgg tagcggaaca
      121 gacgacgacc ggaatcgggg gcatctgaca tcttccgcgg cccgccggga atctgccctg
      181 tcccggctgc cccggctcag ggcatatcga ggtcgtccgt cgaggccaga cccgaggacc
      241 gcgcaaggct gcgctcgatg acgtcgaaga tctcgtcggc gagttggtgg cgcttgtagg
      301 ccccgtagcc cgcggccccg atgccgatgg gccccagcaa ggcatagccc agtaccccac
      361 ccacggcctg gctggtgagg gcgccgcggt ccacagtgac cagcgtgcct tcgccggacg
      421 gcatgagggt aacggtggcc tcacggccga cacccacggc gaaccggacg gtgtccatcc
      481 gcttggccgt caccaggtag ccttcgccct gctcgaacca ctgcgtctcg aaccgctcgc
      541 gctggaagaa gctctcgagg gcggcggcga gagaccggag atcagctcgg acggggtagc
      601 gccggacttc gggcatggcg ggtgcctctc ctgggcacgg gcgggagcta tccgagctcg
      661 agcctggcca cttccatcga cgctgggtcg ttggttccca ggccgcgctg ggcggcggag
      721 tcgatctgct ttgggtgccg gcccaccgcc tcgaggggcg gcagaccgtt cgcctggcgg
      781 gctgcctcaa tcatctgcca gccctgatag tcgagtgcca ccgggtcggt gctcatgagg
      841 atagctccgg gctcgaacac gaactgtggg ttcatcgtcg ggccgccctc gtagcagccc
      901 accgtggcgt cgcacacaat caggcgctgc ttctgtcgga tcagaggaag cgcgttgagg
      961 tcggcgatga acggatcgca cccgtcggcg tggaagtcac ccggccgagc gaacgagccg
     1021 tagtgattct tcagcgagag ggctacgccg gacaggttgt ggtccttgag tatcggcacg
     1081 ttgatgaggg ccgtgatctg ctcagccagg atctgactaa cctcgccttt ccactgaccc
     1141 tggctcatct cggccgtgac cttgccgttc gtgccgaagc atcggacgcc cgggccgtcg
     1201 gtgttgacct cgaagccccc ctggatgagc tcccggtcgc tgcgctccca cacgatgatg
     1261 ctgctcgcgg ggacgccggc atcctgaaga cgccgggtaa ggacgctgac gagggccacg
     1321 ctcgatgaca gaccgaaccc ggccaggcag ttgaccttca ggcccaccac gtcggtgggc
     1381 tcgaacagcg accgccatgc ctctacggcg tcggtcttgc ccgtgagggc cgccatgctc
     1441 tggtcgacca tggctgtaag gactgcagta tcgcgctcgc cgtcgggtgt gaccaccttg
     1501 gagtggtgcg cgatggccac cttgctggac gtctgggcgt ctgcccataa ccccgcacgg
     1561 agtgccaccg ccgcacccgc tgccccggcg atggccttca ggaagtccct tcgcgaatgc
     1621 ccacccaggt tcagcgcgac cgcctccggt atgcacaggc tcatcgggag cccggactga
     1681 tcgtgcccat cagagtcgtt ctagcatgag ttgagacgct cggcaacacc ggggcgttcc
     1741 gacgtggcgg gtccccggtg cgcccggcta gtgtcgagcg agacgctcga cctactctgc
     1801 ctgcagcttc acgacccagt cggcgatctg ctcgcgggcc gcgtcgaggt cgccggacgc
     1861 gagatcagtg tttctgagcc acgcctgggc ctgctcggcc aggccgccct tcgcctccag
     1921 ggcgcgcgcg atggcgcgtt
//
LOCUS       DAOSPL010001009         1939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_59071_length_1939_cov_3.291401, whole genome shotgun sequence.
ACCESSION   DAOSPL010001009 DAOSPL010000000
VERSION     DAOSPL010001009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59071_length_1939_cov_3.291401"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..>1939)
                     /locus_tag="PLQ54_16240"
     CDS             complement(46..>1939)
                     /locus_tag="PLQ54_16240"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOS94870.1"
                     /translation="GRVTLSWTASKSPDVTGYFIYRSAAAGTSYRRLNGAPQRETRYV
                     DESVTNGVTYTYRVSAVDSSVPFNESALTDAVSAVPRDRTPPDTPTGLAAQGANRQVT
                     LKWAANTDQDLAGYNVYRSSTPDGSYARMNSEVVTGTAYTDVQVVNGTVYYYRITAVD
                     TAVPANESEPTDPVVAQPVRVFTLSLPAGAHMLGIPSTNVAGDPRATLHVSHDQWRLA
                     RWWKPDAADASYHFADPTTLDADASFTPPDALSSSPTVSMPPAGIGYWIVLGAPGEVT
                     LSGGTTPGGGYYVVRLYRGWNLVGNPWEGAMNWGAITTSPSGSIQPVGWQWDLDQGYV
                     PLYDVQAANVRSQVAPLTSFWVYATSNCNLVLPGAVPVAARATSAASRSAAHSLFQGV
                     SWKISLSARAGQRRDDANWIGVSDGSASPTSRILGPPPGAGYVDLYVTGSSGERLAAD
                     FRQSGAGTLSWDLRVESDIPNVPVDVLVPDLRELPRDWALVLTDLDSGEEVFLRTTST
                     YRIQAGPGGCRRSLRLVATRDRAGGLRIFGFQSAAVRRGGVSLTFQMTRGAATTVQVL
                     NAAGRVVRTVESDQPRSAGAHELYWDGLADSRSRVPSGMYTIRIGARAADGECTQAVQ
                     VVRLGF"
BASE COUNT          316 a          646 c          675 g          302 t
ORIGIN      
        1 ccgccgttag cagcagacga aacaagaccc gcatcgggac ctccatcaga acccgaggcg
       61 cacgacctgc acggcctggg tgcactcgcc gtccgcggcg cgggcaccga tccgtatggt
      121 gtacattcca ctgggcaccc gggaccggga gtcggccagg ccgtcccagt atagttcgtg
      181 agcacccgca ctgcgtggct gatccgactc cacggtcctc accacgcgac ccgccgcgtt
      241 gagcacctgc accgtcgtgg cggctccccg cgtcatctgg aacgtcagag ataccccacc
      301 ccgccgcact gcggccgact gaaacccgaa gatcctgagc cccccggcgc ggtcgcgggt
      361 ggctaccagg cgaaggcttc ggcggcagcc gcccgggcct gcctggatac gataggtgga
      421 agtggtccgc aagaagacct cttcaccact gtccagatcg gtcaagacca gggcccagtc
      481 gcgcggcagc tcgcgcagat ccggtactag gacgtccacc ggcacgttcg ggatatcgga
      541 ttcgactcgg agatcccagg acagtgtgcc cgccccagac tgccggaagt ccgcggccag
      601 tcgctcgccc gaggagcccg tcacgtagag atcgacgtag cccgctcctg gcggcgggcc
      661 caagatccgt gaggtcgggc tggcgctgcc gtcgctcacg ccgatccagt tggcgtcgtc
      721 gcgacgctgg ccagctcggg cggagagact gatcttccac gagactccct ggaacaggga
      781 gtgcgctgcc gagcgggatg ccgcagaggt ggcgcgggcg gcgaccggga ccgcgccagg
      841 cagcaccagg ttgcagttgc tggtggcgta gacccagaac gatgtcagcg gggcgacctg
      901 cgaccgtacg ttggccgcct gaacgtcata cagtggcaca tagccctggt ccagatccca
      961 ctgccacccc acgggctgta tcgaacccga gggtgaggtg gtgatggcgc cccagttcat
     1021 ggcgccctcc catgggttcc cgaccaggtt ccacccgcgg tacagccgga ccacgtagta
     1081 cccgccgccg ggcgtggtgc ccccactcag cgtgacttcg cctggcgcgc ccagcacgat
     1141 ccagtaacca atccccgccg gcggcatcga gaccgtggga cttgagctca gcgcatccgg
     1201 aggcgtgaag gaggcgtcgg cgtccagggt ggtcgggtcg gcgaagtggt acgaggcatc
     1261 tgccgcgtcg ggcttccacc atcgagcgag ccgccattgg tcgtgcgaga cgtgaagcgt
     1321 cgctctcggg tcgccggcca cattcgtgga tgggataccc agcatgtggg cgccggccgg
     1381 gagcgagagc gtgaagacgc gaaccggctg ggccaccacg gggtcggtgg gctcgctctc
     1441 gttggcgggg accgccgtgt cgacggctgt gatgcggtag tagtagaccg tgccgttcac
     1501 gacctgcaca tcggtgtagg cggtgccggt caccacctcg gagttcatcc gggcatagga
     1561 gccatcgggg gtcgacgagc ggtagacgtt gtagcccgcc aggtcctggt ccgtgttggc
     1621 ggcccacttc agggtgacct gccggttggc gccctgggcc gcgaggccgg tgggtgtgtc
     1681 gggtggcgtc cgatctcgag gcactgcgct caccgcgtcc gtgagcgcac tctcgttgaa
     1741 cgggaccgac gagtcgacgg cggacacccg gtaggtgtag gtgacgccat tggtgaccga
     1801 ctcatccacg tagcgcgtct cgcgctgtgg cgcgccattg agccgccggt aactcgttcc
     1861 cgccgccgca ctgcggtaga tgaagtaacc ggtgacatcg ggggacttgc tggcggtcca
     1921 tgagagggtg acacggccg
//
LOCUS       DAOSPL010001010         1938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_59125_length_1938_cov_2.689857, whole genome shotgun sequence.
ACCESSION   DAOSPL010001010 DAOSPL010000000
VERSION     DAOSPL010001010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59125_length_1938_cov_2.689857"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PLQ54_16245"
     CDS             complement(<1..536)
                     /locus_tag="PLQ54_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94871.1"
                     /translation="MVTGLLAVALVGLVVAGSGAFGMTLAHDGKAKCPIVVARSAIAA
                     ERTAAQDLASILHQVTGATFEIRAPGEVAEGERQIIVGPSARTRHLAPDVDVAALGSD
                     GIVIRTVGDRLLLFGGRPRGTIYAVNTFLEDVVGCRWWTSAESTIPRKPTLEVAGLHT
                     VYTPKLFSREVFFKDAFEP"
     gene            complement(618..1394)
                     /locus_tag="PLQ54_16250"
     CDS             complement(618..1394)
                     /locus_tag="PLQ54_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94872.1"
                     /translation="MAPLRPVWLIAIILPLPVAADSPERSLGPVETRPATAYVSRLLS
                     PARPVASGKRLDLWVARELSPLFDGELVAEEWRGALELNVPWATQETTAKGFPPRVVF
                     CLARQRDADGLLLGCIVQDATRETTLAPRPQPASAEGATAPTDELSRWAGVDHLFVGL
                     YAKGSERPVLASLRLCRGATVAEDGLLVRQGAVGEIAETGWAAELQLSSGALDIADGD
                     EVGLFVQVHNGAGPDAAWPAGARADDPATWGRMVTRRPGG"
     gene            1559..>1938
                     /locus_tag="PLQ54_16255"
     CDS             1559..>1938
                     /locus_tag="PLQ54_16255"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS94873.1"
                     /translation="MRKRAPHLQETWLRPQKRAAAGRVMVLAYPNLYRLGMSNLGFQT
                     IRQIAAETAGWSCDRLFLPDADVSRSPRRLRTLDFGMAPADADLLAFSVSYELDYVNV
                     ARILALSGVPLRQAERDERWPLVAV"
BASE COUNT          295 a          702 c          649 g          292 t
ORIGIN      
        1 ggctcgaagg cgtccttgaa gaacacctcg cggctgaaca gcttcggcgt gtagaccgtg
       61 tggagccccg cgacctcgag cgtgggcttc ctcgggatcg tgctctcggc ggacgtccac
      121 catcggcatc ccaccacatc ctcgaggaag gtgttgacgg catagatggt cccgcgaggg
      181 cgcccgccaa acagcaggag ccggtcgccc acggtgcgga tgacgatccc gtcggagccc
      241 aatgccgcga catcgacatc gggcgcgagg tgccgcgtgc gcgccgaggg acccacgatg
      301 atctggcgct cgccctcggc cacctcgccg ggggcgcgga tctcgaacgt tgcccccgtg
      361 acctggtgca ggatcgacgc taggtcctgg gccgccgtcc gttcggcagc gatggcgctc
      421 cgcgccacca cgatggggca cttcgctttg ccgtcatggg caagagtcat cccgaaggca
      481 ccggatccgg ccacgaccag ccccaccaag gccactgcca acagtcccgt caccatggtc
      541 cctccctagc tgttccgagc gtgacttcgg cgcatcgtgg cggagggcct gccgctgggc
      601 gacgcggagc cggggcgtta gccgccgggc cggcgggtga ccatgcgccc ccaggtggcc
      661 gggtcgtcag cccgggcacc cgcgggccag gcggcgtcag gcccggcccc gttatggacc
      721 tggacgaata ggccgacctc gtctccgtcg gcaatgtcca gggcgccact cgagagctgg
      781 agctcggccg cccaaccggt ctccgcaatc tctcccacgg cgccctgtcg cacgagtagg
      841 ccgtcctccg ccaccgtggc gccgcggcac aggcggaggc tcgccaatac gggacgttcc
      901 gagcccttgg cgtacaggcc gacgaacaga tgatcgaccc ccgcccagcg agacagttca
      961 tccgtcgggg ccgtcgcgcc ctcggccgaa gccggttggg gccgtggcgc caacgtggtc
     1021 tcgcgtgtcg catcctgcac gatgcatccg agcagtaagc catcggcatc ccgctgtcgg
     1081 gccaggcaga acaccacccg cggcgggaac cccttcgccg tcgtctcctg ggtcgcccag
     1141 gggacgttca gctcgagggc gccgcgccat tcctcggcga ccagctcacc atcgaagagc
     1201 ggcgagagtt cgcgggcgac ccagagatcc agccgcttcc ccgaggcaac cgggcgcgcg
     1261 gggctcagca atcgcgagac gtacgccgtg gccgggcggg tctcgacggg accaagggat
     1321 cgctcaggtg agtctgctgc tacgggaagc ggcaggatga tcgcgatcag ccagaccggt
     1381 cgcagcgggg ccaccccctt ccggaatgac gtcggtccga acggccgacc tacgcgaatc
     1441 aacgcgtgat gtcgcggccc gggtccctat ccttcgcggg cgggccccgc agccccttgc
     1501 cgggcgccag gtgccagcct actctggaga gacgcccgac tgaatgggac ggagcgccgt
     1561 gcgcaagagg gcaccacatc ttcaggagac ctggcttcgg ccccagaagc gcgcggccgc
     1621 cgggcgcgtg atggtgctcg cctaccccaa cctctaccgc ctgggcatgt cgaaccttgg
     1681 tttccagacc atccgccaga ttgcggccga gacggcgggc tggtcgtgtg accggctgtt
     1741 cctgcccgat gccgatgtct ctcggtctcc gcgtcggctc cgcaccctgg acttcggtat
     1801 ggcgcccgcc gatgccgatc tcctcgcttt cagcgtctcc tatgagctgg actacgtgaa
     1861 cgtcgcgcgc atactcgccc tcagtggtgt tccgctccgc caggcggaac gcgatgagcg
     1921 atggcccctc gtggccgt
//
LOCUS       DAOSPL010001011         1935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_59259_length_1935_cov_2.855851, whole genome shotgun sequence.
ACCESSION   DAOSPL010001011 DAOSPL010000000
VERSION     DAOSPL010001011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59259_length_1935_cov_2.855851"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1491)
                     /locus_tag="PLQ54_16260"
     CDS             complement(<1..1491)
                     /locus_tag="PLQ54_16260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOS94874.1"
                     /translation="MGRRALVPVWALVFGLLVVAMQAVFDAVLFHADAAVRHPLLQPP
                     DFELYLRTVMLALCVLTGVVVAVYLRRRAQVEEALRVSEQRYRALFDDACDAVIIYDD
                     QGRITDANRSASELLGCPIEAIRELRIGDIGLPQGDPAHPGGADADGRQPAVNVQRLH
                     RPDGTWVDVEVTSRRLPVAAELTQAVLRDVTAQQAGERALLASLEAERRLAEGLVAVT
                     TAMLELSTCRSTDDLARSAVELGRQSLPLDRIGLWFTSDDGEFLIGTYGTDENGNTRD
                     ERHERHRLVPQENPHQMMSEQRRWRLVSDVDLYDHNHRVVGRGSSVRAALWDGERVTG
                     MLCGDNLLSGRTITERDCELVALYAAIIGHLASRLKIDEERARLQAQVQDARKLESLR
                     ILAGGVAHDFNNILMAIIGNAELALSRMEPDSPLRHFVDEIEAAASRAAVLSRQMLTY
                     SGHAALKVEPIDLSLLVQRMSLVLRSSVSSRAALHYELGGSPCAVLG"
     gene            complement(1660..1733)
                     /locus_tag="PLQ54_16265"
     tRNA            complement(1660..1733)
                     /locus_tag="PLQ54_16265"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1697..1699),aa:Val,seq:tac)
     gene            complement(1784..1858)
                     /locus_tag="PLQ54_16270"
     tRNA            complement(1784..1858)
                     /locus_tag="PLQ54_16270"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1823..1825),aa:Glu,seq:ttc)
BASE COUNT          362 a          642 c          642 g          289 t
ORIGIN      
        1 gcccagcacc gcgcaggggc tccctccgag ttcatagtga agggcggctc gagacgagac
       61 tgatgaccgc aggacgagcg acatccgctg gaccagaagt gacagatcga tcggctcgac
      121 cttgagggcc gcgtggccgg agtacgtcag catctggcga gacagaacgg ctgcccgcga
      181 cgctgcggcc tcgatctcgt cgacgaaatg gcgcagcggc gagtcgggct ccatacgcga
      241 gagtgccaac tcggcattgc cgatgatggc catcaggatg ttgttgaagt catgggccac
      301 accaccggcc aggatccgca ggctctcgag cttgcgggcg tcctggacct gggcctgcag
      361 gcgggcacgc tcctcgtcga tcttgaggcg cgacgccaga tggccgatga tcgcggcata
      421 gagggcaacc agctcgcagt cccgctcggt gatggtccgc ccggacagga ggttgtcgcc
      481 acagagcatc cccgtaactc tctcgccatc ccacagcgcg gcccgcaccg aggacccacg
      541 accgacgaca cggtggttgt ggtcgtacag gtcgacgtcc gagaccagtc gccagcggcg
      601 ctgctcagac atcatctggt gagggttctc ctgaggcacg agccgatgac gctcgtgccg
      661 ttcgtcccgc gtgttgccgt tctcatcggt gccgtaggtc ccgatcagga actccccgtc
      721 gtcactggtg aaccacaggc cgatgcggtc cagggggagc gactggcgcc ccagctcgac
      781 cgcactgcgc gccaggtcgt cggtcgaccg gcacgtggag agctcgagca tggcggtcgt
      841 gacagcgaca aggccctcgg cgagccgccg ctcggcctcg aggctcgcca gcaaggcgcg
      901 ttcgcccgct tgctgagcag taacatcccg caagacggcc tgcgtcaact cagccgcgac
      961 gggcaggcgg cgcgaggtca cctcgacatc gacccaggtg ccatcggggc ggtggaggcg
     1021 ctgaacgttc acggcgggct gccgcccatc ggcatcggcg ccccccgggt gcgcaggatc
     1081 gccctgcggg aggccgatgt caccgatgcg gagctccctg atggcctcga tcgggcaccc
     1141 cagcaactcg gacgcacttc ggttggcatc ggtgatgcgg ccctggtcat cgtagatgat
     1201 gacggcgtcg caggcatcat caaagagggc gcgatagcgc tgctcgctga cccgcagcgc
     1261 ttcctcgacc tgggcccggc gacgcaggta gacggccacg acaacgcccg tcaagacaca
     1321 gagcgccagc atgacggtcc gcagatagag ctcgaagtcg ggcggttgta gcagcggatg
     1381 cctgacggcc gcgtcagcat ggaacaggac ggcgtcgaag acggcctgca tggccaccac
     1441 gaggaggccg aacaccagag cccagaccgg tacgagcgcc ctccgcccca tgcttggcct
     1501 ccccccccac acgcacaggc ccatcggcgt gtcggcggcg gcgcctatga gccgttatcg
     1561 gccggacaca ttgacaagta taccgtaccc gaggctgcgc ccactctggc tgacgactcg
     1621 gcggggacag gagcaccagg aggcatctcc ggcggcaggt gggcgaggtg cgactcgaac
     1681 gcacgacctt ctgcttgtaa ggcagacgct ctaaccgact gagctactcg cccacagaga
     1741 ggataaggca acgcaggcca cagctgcaga tggtggtatg ggatggtacc ccggacggga
     1801 attgaacccg tactacggcc ttgaaagagc cgtgtcctga ccattagacc accggggcgt
     1861 gctcactcac gttcgttggt gacccgggga ggacttgaac ctccgaccaa cggattaaaa
     1921 gtccgctgct ctacc
//
LOCUS       DAOSPL010001012         1932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_59387_length_1932_cov_3.557805, whole genome shotgun sequence.
ACCESSION   DAOSPL010001012 DAOSPL010000000
VERSION     DAOSPL010001012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59387_length_1932_cov_3.557805"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PLQ54_16275"
     CDS             complement(<1..476)
                     /locus_tag="PLQ54_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS94875.1"
                     /translation="MATRTGWTGPRPGSARGFTLIELLVVIAIIAILASIVMPVYSKA
                     REKAVSVSCLSNIKQIGTAATMYAHDWDLKFPSGLYLTGVGESWWRWPDMLQPYVQNY
                     QLFFCPDSVEIQDTIIWGQYAYNAEWVLVQHTQARVPMHLKITNTKRPSEQILFTET"
     gene            805..>1932
                     /locus_tag="PLQ54_16280"
     CDS             805..>1932
                     /locus_tag="PLQ54_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS94876.1"
                     /translation="MLGWDVRPEGPKDETPRGVSGTERQRLRERLDDSLSLARAMAGP
                     ADPRGLADACRDVIELADALGDPAAKANAFQMLGQAYLQLGELDGAREAYEQAAGLWQ
                     GIGAVDRELQTRQALAGVLLDLGDPHAALNQASQLADAPDWVHRCLGLLTQSHVRQRL
                     GQFGLALAGLEEAQRTVSRAEATFRAHVHQLEASLHLSRGDTKAALASAQAMADAGAS
                     AASAPLRLDALILMGTAHTRRGEMAEGWQPLDQALQVAELAGDAERTARVNVALSEWF
                     ALAGLCEPALTHAYAAQRGARACASHSAELEAELALAEAFLAAEDLDNAEGPIARASE
                     LAEGSKDFEGHVRAELLKGRLAAAAGDHRAAIALLSQVMTEA"
BASE COUNT          314 a          608 c          713 g          297 t
ORIGIN      
        1 gtctcggtga agaggatctg ctccgacggc cgcttggtgt tggtgatctt gaggtgcatg
       61 gggacccggg cctgggtgtg ctggaccagg acccactccg cgttgtaggc gtactggccc
      121 cagatgatgg tgtcctggat ctcgacggag tcagggcaga agaacagctg gtagttctgg
      181 acatagggct ggagcatgtc gggccaacgc caccaggact cgccgacccc tgtcaggtac
      241 aggccgctcg ggaacttgag gtcccagtcg tgggcgtaca tggtcgccgc ggtgccgatc
      301 tgcttgatgt tggagaggca actgaccgag acggccttct cgcgtgcctt cgagtacacg
      361 ggcatcacga tggaagccag gatggcaatg atcgcgatca caacgagaag ctcgataaga
      421 gtgaagcccc gggccgaacc ggggcgcgga cccgtccacc cggtgcgcgt ggccataaag
      481 ggacctcctt gggcgtcggc ccgtgggaag accgaaaaga gacctaatgc tcagcatacc
      541 cctgcagacg ttttctcacg cggtgtcgtt cagaaggacc cagacatgga acccgcaaag
      601 aaaggagctg cggggcgtgg gaacgcggtt gacggcgggc ggtttccaac gtaaaatcct
      661 ggtatcacgc ccaacacgtg aatgtcaagc gtcgctccgc aatcgtgaga ggcgcggatc
      721 gggtcagggg tggcgacggg gttggcgggc tcgggccatg gtggcccgag cggtggggtg
      781 ggagtctggg gagggccggc ttcagtgctg ggttgggatg tgcgcccgga aggtcccaag
      841 gacgagacgc ctcggggcgt atccggtacc gagcggcagc gcctacgtga gcggctcgac
      901 gactcgctct cgctggcgcg ggccatggcg gggcccgcgg acccgcgggg gctcgccgac
      961 gcttgccgcg atgtgatcga gctcgccgac gccctcggcg acccggctgc caaggccaac
     1021 gcgttccaga tgcttggcca ggcatatctg cagctcggcg agctcgatgg agcccgggag
     1081 gcctatgagc aggccgccgg cttgtggcag ggcatcggtg ccgtagaccg cgagcttcag
     1141 acgcgtcagg ccttggccgg cgtgctcctc gatctcggcg accctcacgc ggcgctgaac
     1201 caggccagcc aactggctga cgcgcccgac tgggtacacc ggtgcctggg tctgctgacg
     1261 cagagccatg tgcgccagcg cctggggcag tttggtctgg ccctcgcggg tctggaggag
     1321 gcccagcgca ccgtatcgcg ggccgaagcg accttccgcg cccatgtgca tcagctcgag
     1381 gcatccctgc acctctcgcg gggcgacacg aaggccgcgc tggcgtcagc gcaagcgatg
     1441 gccgacgccg gcgccagcgc cgcgtcggcg cccctgcggc tggatgcgct gattctgatg
     1501 gggacggcgc acacgcgccg gggtgagatg gcggagggct ggcagcctct cgaccaggca
     1561 ctccaggtgg cggagctggc cggagatgcg gagcgcaccg cccgggtcaa tgtggcgctc
     1621 tcggagtggt tcgccctggc cggtctctgc gagccggcgc tgacccatgc ctacgccgcc
     1681 cagcgaggtg cccgcgcttg cgccagccat tcggctgagc tggaggcgga actcgcgctg
     1741 gccgaggcct tcctcgccgc tgaagacctg gacaacgcag agggccccat cgctcgggcg
     1801 agcgagctgg cggagggatc caaggacttc gagggccacg tccgcgccga gctgctgaag
     1861 ggccgtctgg cggccgccgc gggcgaccat cgggccgcca tcgcgctgct cagccaggta
     1921 atgaccgaag cc
//
LOCUS       DAOSPL010001013         1932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_59390_length_1932_cov_3.275972, whole genome shotgun sequence.
ACCESSION   DAOSPL010001013 DAOSPL010000000
VERSION     DAOSPL010001013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59390_length_1932_cov_3.275972"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PLQ54_16285"
     CDS             <1..1131
                     /locus_tag="PLQ54_16285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOS94877.1"
                     /translation="ATEVAAKEARVQAFLREQRLEAVFLAQYRNYSWVTGGSEDTIVM
                     AAESGPVTLVLTRDGSKYALCANDEEARNRDEELAGLGYEMISWEWFHNFDATSPQEE
                     FARSKGWEPAEIGSDMPVAWATDVAKQFAPLRFALTEPEMKKYRWLGRECTEACTETC
                     REIRPGMTEKQIQRVIADKLMARGITPTVLLIAVDGRLFQHRHAIATDRKLEKYAQVN
                     ICARKWGLVIAVTRYVHFGPIPAELGHRLRACARVTGAFLSALKPGVTAGEVFQAGIE
                     MYARTGFPDEWRQHHQGGATGYAEREWVTFPGDPTPIVGDQAFAFNPTIMGAKVEDTV
                     ILHADGTLENVTQTLDWPNIPIALTGPAAGQTYYAPSILVRH"
     gene            1205..>1932
                     /locus_tag="PLQ54_16290"
     CDS             1205..>1932
                     /locus_tag="PLQ54_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94878.1"
                     /translation="MPPAAGTAPALELLIRSESGDGYTVCHYASHEGGLRYRPCLVPP
                     EQYEVNCIAVADGEPPVYLVRSLETGAIVQLSAAAHGVFRGMDGRASLLDLVTTLFVS
                     TGALSPAEVRQTVTELQRAGLLVTRPQRHLRASPWRRAAATLGIPGANRGIAALYRCA
                     APVLFSAWAPWFALVIVALGVFANAALPWRSTATALRTHWEVGAAATAVVLGLVLPAH
                     ELAHAFVCRRFGRRVKALGVRLLWG"
BASE COUNT          297 a          649 c          664 g          322 t
ORIGIN      
        1 gccaccgagg tcgccgcgaa ggaggcccgg gtccaggcgt ttctgcgcga acagagactc
       61 gaggcggtgt tcctggccca gtaccgcaac tactcctggg tcaccggcgg gtcggaagac
      121 accatcgtca tggccgcgga gtcgggcccg gtcactctgg tgttgacgcg ggacggctcc
      181 aagtacgccc tgtgcgccaa cgacgaggag gcccggaacc gggacgagga actggcgggc
      241 ctcgggtacg agatgatctc gtgggagtgg ttccacaact tcgatgccac ttcgccgcag
      301 gaggagtttg cccggagcaa aggctgggag cccgccgaga tcggctcgga catgccggtt
      361 gcatgggcga ccgacgtcgc caagcagttt gcgccgctgc gctttgcgct caccgagcct
      421 gagatgaaga agtaccgctg gctcggtcgc gaatgcacgg aggcgtgcac cgagacatgt
      481 cgcgagatcc gaccgggcat gaccgagaag cagatccagc gcgtcatcgc cgacaagctc
      541 atggctcgtg gcatcacgcc taccgtgctc ctcattgctg tcgatggtcg gctgttccag
      601 caccgccacg ccattgcgac ggacaggaag ctcgagaagt atgctcaggt gaacatctgc
      661 gccaggaagt ggggcctcgt catcgccgtc acgcgctatg tccacttcgg tcccatcccg
      721 gcggagctgg ggcaccggct ccgggcctgt gcccgggtga ccggcgcgtt cctgagcgcc
      781 ctgaagcccg gtgtcacggc cggtgaagtc ttccaggccg gtatcgagat gtacgcgcga
      841 actgggttcc ctgacgagtg gcggcaacac caccagggcg gcgccacggg ctatgcggag
      901 cgcgagtggg tcaccttccc cggcgatccg acacccatcg tgggcgacca ggcattcgcg
      961 tttaacccga ccattatggg tgccaaggtg gaggacacgg ttatcctcca cgccgacggg
     1021 actctcgaga acgtcaccca gacgctcgac tggcccaaca tccccatcgc cctcaccggg
     1081 ccggcggctg gacagaccta ctatgcgccc agcatcctcg tccgtcacta gggacgcctg
     1141 agcatgagac gagacggagc cggggcgtcc gccttggctc cgttcgcgcg agacggagga
     1201 gaccatgcct ccggcagcgg gcaccgcgcc ggctctcgag ctgctgattc gcagtgagag
     1261 cggcgatggg tatacggtgt gccactacgc ctcgcacgag ggtggcctgc ggtaccggcc
     1321 ctgcttggtg ccgcccgagc agtatgaggt gaactgcatc gccgtggcag acggcgagcc
     1381 gcccgtatat cttgttcgaa gcctggagac cggcgccatc gtccagctgt ccgcggccgc
     1441 ccacggggtg ttccgcggca tggacggccg ggcgagtctt ctagacctcg tgaccacatt
     1501 gttcgtgagt acgggcgcgc tgagcccggc cgaggtgcgc cagaccgtga ccgaactcca
     1561 gcgcgccggt ctgctcgtca cccggcctca gaggcatctg cgggcatcgc cctggcggcg
     1621 cgcggccgct accctgggga taccgggcgc caaccggggc atcgcggccc tctaccgctg
     1681 cgcggcaccc gtcttgttct cggcctgggc gccgtggttc gcgttggtca tcgtggccct
     1741 gggcgtcttc gccaacgccg ctctgccctg gcggtccacc gcgaccgcgc tccgcacgca
     1801 ctgggaggtc ggggcggcgg ccaccgcggt cgtgcttggg ctcgtcctcc cggcgcacga
     1861 gttggcccat gccttcgttt gccggcgctt cggtcgtcgt gtgaaggcgc tgggtgtacg
     1921 cttgctctgg gg
//
LOCUS       DAOSPL010001014         1932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_59398_length_1932_cov_2.911561, whole genome shotgun sequence.
ACCESSION   DAOSPL010001014 DAOSPL010000000
VERSION     DAOSPL010001014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59398_length_1932_cov_2.911561"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLQ54_16295"
     CDS             <1..685
                     /locus_tag="PLQ54_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94879.1"
                     /translation="LPDQLNALRARASGWTQQALQRLFPSLPGLTLAQLAGTMRHGFA
                     VCTREFVNWGVDPWAAIEALLPGADSEGTLARTAIRDTCPDGQAWIGVAVFPGSGFFS
                     EQSQLDPVTDESGSPADWMVYALGLLPGGRSIAYEVLTAEDCATYGFRIEAESEWSEL
                     LRCLRRVQFRREPISVAEADLATAKAGRYALAARVVPELQWLRQRFLGRALHTSVDAW
                     LDAVRRWQD"
     gene            complement(702..1727)
                     /locus_tag="PLQ54_16300"
     CDS             complement(702..1727)
                     /locus_tag="PLQ54_16300"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOS94880.1"
                     /translation="MDATGADRMALVSIQDPRAGAGLGPSLVAKALHPDRFYVFAGPN
                     HAQHLSGGAVVTPSLAAQAQRFARIGCDGMKLLEGKPTSRREVDVPITDPYFAEFWEC
                     VEGLGTPLIWHVGDPETFWDPARIPGWARDNQWGYGPEDVALEVLYTEVDAVLARHPR
                     LRVAFAHFYFLSADLDRAGRFLDDHPTVSFDLAPGIEMLYNMSEEPVRAREFFIRYAD
                     RIVFGTDIWSGMALEEARHRAGMVHRWLVTEDEFRVSPNADYLLGPPEDGIIRGMALP
                     DDVLERVFCRNFERFAGVRPRPLDAAGAADVCDEIARAAQGLSGTPTSETQAGQAADR
                     LRAVASD"
     gene            complement(1749..>1932)
                     /locus_tag="PLQ54_16305"
     CDS             complement(1749..>1932)
                     /locus_tag="PLQ54_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94881.1"
                     /translation="DHEHDTTDWPLNANRTPACAGLGRTLTKPPDDFEGGGDMNEARA
                     WKGPILDGHVHMGDLG"
BASE COUNT          297 a          645 c          679 g          311 t
ORIGIN      
        1 cctgccggat cagcttaacg cgcttcgcgc ccgcgccagc gggtggaccc aacaggccct
       61 gcagcgcctg ttcccctcgc tgcccggcct gaccctggcg cagctggcgg gtaccatgcg
      121 ccatgggttc gcggtgtgca ctcgcgagtt cgtgaactgg ggagtcgatc cctgggcagc
      181 gatcgaggcg ttgctgcccg gcgccgactc tgagggtact ctggcgcgca ccgccatccg
      241 cgacacgtgc ccggatgggc aggcgtggat cggtgtggcc gtgtttccgg gctcgggctt
      301 cttctccgag cagagccagc tcgatcccgt gaccgacgag tccggcagcc ctgccgattg
      361 gatggtctac gccctcggtc ttctgccggg gggccggtcc atcgcctatg aggtgctgac
      421 agccgaggac tgcgcgacct acgggttccg gatcgaggcg gagagtgagt ggagtgagct
      481 gctgcgatgt ctgaggcggg tgcagttccg ccgcgagccg atctcggtcg ccgaggcgga
      541 cctggcgacc gccaaggcgg gacggtacgc tctggcggcc cgcgtggttc ccgagctgca
      601 gtggctccgg caacgcttcc tcggccgggc cctccatacg agcgtcgacg cctggctgga
      661 cgcggtgcgg cggtggcagg actgacgcgg gcgtcgtcag gctagtccga agcaaccgcc
      721 cgcagccgat cggccgcttg gcccgcctgg gtctcagacg taggcgtgcc ggagagccct
      781 tgggccgcac gggcgatctc atcacacaca tcggccgcac cggcagcatc gagcggccgg
      841 ggtcggacgc ccgcgaaccg ctcgaagttg cggcagaaga cgcgctccag aacgtcgtcg
      901 ggcagtgcca tgccgcggat gatcccgtcc tcgggtgggc ccaggaggta gtcggcgttg
      961 ggcgacactc ggaactcgtc ctcggtgacc agccagcgat gcaccatgcc ggcccggtgt
     1021 cgcgcctcct ccagggccat tccgctccat atgtcggtgc cgaagacgat gcggtcggcg
     1081 tagcggatga agaactcccg cgcgcggacg ggctcttccg acatgttgta gagcatctcg
     1141 atcccggggg ccaggtcgaa gctcaccgtc ggatgatcgt cgagaaagcg ccccgcccgg
     1201 tcgaggtcag ccgagaggaa gtagaagtga gcgaacgcca ctcggagccg agggtgtctc
     1261 gcgaggacgg cgtcgacctc cgtgtacagc acctcgagcg ccacgtcttc gggaccgtag
     1321 ccccactggt tgtcacgagc ccagccgggg atccgggccg gatcccagaa ggtttcggga
     1381 tcgcccacat gccagatcag gggcgtgccg agtccctcga cgcactccca gaactcggcg
     1441 aagtaggggt cggtgatggg gacgtccacc tcgcggcgcg aggtcggctt cccctcgagc
     1501 agcttcatcc catcgcaccc gatgcgagcg aagcgctgag cctgtgcggc gagagagggc
     1561 gtcacaacgg cgccgccgga caggtgctga gcgtggttgg gccctgcgaa cacgtagaag
     1621 cggtccgggt gaagcgcctt ggcaaccagg gagggcccca acccggcccc cgcacgcggg
     1681 tcctggatcg ataccagagc catgcgatcg gccccggtcg cgtccatgat cccaagcagg
     1741 ggctcccgct agccgaggtc gcccatgtga acatggccat ccaggattgg acccttccag
     1801 gcccgggctt cgttcatgtc gccgcctcct tcgaagtcgt ccggaggctt cgtaagggtg
     1861 cgcccgagac ctgcacaggc gggtgtccga ttcgcgttca atggccagtc tgtcgtatca
     1921 tgctcgtgat cg
//
LOCUS       DAOSPL010001015         1929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_59504_length_1929_cov_3.502668, whole genome shotgun sequence.
ACCESSION   DAOSPL010001015 DAOSPL010000000
VERSION     DAOSPL010001015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59504_length_1929_cov_3.502668"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..515
                     /gene="nuoK"
                     /locus_tag="PLQ54_16310"
     CDS             168..515
                     /gene="nuoK"
                     /locus_tag="PLQ54_16310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOS94882.1"
                     /translation="MLGDILESLGQPVGLRHCLVFAALLFAVGLYGVLTRRNAIGILM
                     ALELMLNAVNANLVAFWRYGPGPREALNGQVFAVFVITMAAAEAAVGLAIILALFRLR
                     RTVNADEVNLLKG"
     gene            527..>1929
                     /locus_tag="PLQ54_16315"
     CDS             527..>1929
                     /locus_tag="PLQ54_16315"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOS94883.1"
                     /translation="MDLLSQHAYLIAVLPVLAFGAVLLLGQKAPGRGAYLPIAALAIG
                     TVWSIYLCARIHSGAALPQGRLLWGVGSTGFYISFLVDGGGAAMLTMVCSVALLIVIY
                     SQGYMRGDAHYSRFFAYLSLFSASMLGFVLSSNLLLMFICWELVGLMSYLLIGFWFHK
                     PEAARACKKAFMTTRLGDLGITLAVVALYHEAGTLDLRVIFEKVAAGGLDTPMAFWIA
                     MGLFAGAVGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVFLVWRTFPVFAATA
                     DYALMPYCAPFWVALIGGFTALFASTMAVVQDDIKRVLAYSTISQLGYMMLALGCSAL
                     FAGLFHLLTHAFFKACLFLGAGSVIIGTHHVQDMRKMGGLRVKMPVTFWTFVIATAAL
                     CGLGIPGVVGLSGFYSKDAVLAAAKVAEYPGEVAVVLYGFGQVGALLTAFYMTRCVML
                     TFMTSPRDAHVFEHASET"
BASE COUNT          256 a          596 c          660 g          417 t
ORIGIN      
        1 agagtctggc cgatgacctg atccgccact atgtgccggc gtttgagatt gcctccgtgc
       61 tgctgctggt ggcgatgatc ggtgcggttg tgctgacgct cagggccaaa cagcgcgagg
      121 gccctccgac cgacgatgag tgacacgcag gaaggcgatt gggcttcatg ctgggtgaca
      181 tactggagag cctggggcag ccggtggggt tgcggcactg cctcgtgttc gcggccctgc
      241 tgttcgccgt gggcctctat ggggtgctca cgcggcggaa cgccatcggc atcctgatgg
      301 cgctggagct gatgctcaac gcggtgaatg cgaatctcgt ggcattctgg cggtatgggc
      361 ccggccctcg agaggccctg aatggtcagg tgttcgcggt gttcgtgatc accatggcgg
      421 ctgccgaggc ggcagtgggc ttggccatca tcttggcctt gttccgcctg cgccgcacgg
      481 tgaatgcgga tgaagtgaac ctgctgaagg gttagcgcac ccggagatgg atctactctc
      541 ccaacatgcc tatctgatcg ccgtactgcc ggtgttggcc ttcggggcag tgctcctgct
      601 gggccagaag gcgccggggc ggggcgcgta cctgcccatt gcggccctgg ccatcggcac
      661 cgtgtggtcg atctatctgt gtgcgcggat ccactcgggc gctgccctgc cccaggggcg
      721 cctgttgtgg ggggtcggat cgaccggctt ctacatcagc tttcttgtcg acggcggcgg
      781 ggcggccatg ctcaccatgg tatgctcggt ggccctgctg atcgtgatct actctcaggg
      841 gtacatgcgg ggcgacgccc actactcgcg gttcttcgcc tacctgtcgt tgttctcagc
      901 gtcgatgctc ggcttcgttc tctcgagcaa cctgctgctg atgttcatct gctgggagct
      961 cgtgggattg atgagttatc tgctcatcgg gttctggttt cacaagcccg aggcagcccg
     1021 ggcgtgcaag aaggcgttca tgaccacacg gctgggggac ctggggatca ccctggccgt
     1081 ggtggcgttg taccacgagg ccgggacgct cgatctgcgg gtgatcttcg agaaggtcgc
     1141 cgcaggcggc ctggacacgc ccatggcctt ctggattgcc atggggctgt tcgccggtgc
     1201 ggtcgggaaa tcggcccagt tccccttgca tgtgtggttg cccgatgcca tggagggccc
     1261 gactccggtc tccgcgctca tccacgcggc gaccatggtg gccgcgggcg tgttcctggt
     1321 ctggcggacc ttccctgttt tcgcggccac ggccgactac gcgttgatgc cctactgtgc
     1381 gcccttctgg gtggccctca tcggcggctt caccgccctg ttcgcgtcca ccatggccgt
     1441 ggtccaggat gacatcaaac gggtgctggc gtactcgacc atcagccagc tcgggtacat
     1501 gatgctcgcc ctgggctgca gcgccctgtt cgccgggctg ttccatctgc tgacccacgc
     1561 gttcttcaag gcgtgcctgt tcctcggggc cggcagcgtc atcattggca cccaccatgt
     1621 tcaggacatg cggaagatgg gcggcctgcg ggtgaagatg cccgtcacgt tctggacctt
     1681 cgtcatcgcc acggccgccc tgtgcgggct gggcattccg ggcgtcgtgg gcctctcggg
     1741 cttctacagc aaggacgcgg tgctggcggc cgccaaggtg gcggagtacc cgggcgaggt
     1801 cgcggtggtg ctctacggct tcggccaggt gggagccttg ctcacggcgt tctacatgac
     1861 ccgctgcgtc atgctcacgt tcatgacgag tccccgcgat gctcacgtgt tcgagcacgc
     1921 ctccgagac
//
LOCUS       DAOSPL010001016         1926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_59650_length_1926_cov_2.364511, whole genome shotgun sequence.
ACCESSION   DAOSPL010001016 DAOSPL010000000
VERSION     DAOSPL010001016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59650_length_1926_cov_2.364511"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            529..>1926
                     /locus_tag="PLQ54_16320"
     CDS             529..>1926
                     /locus_tag="PLQ54_16320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOS94884.1"
                     /translation="MVTEPDSGFEPIASALVAYHPEADLDLIRRAYHYAVRSHEGQTR
                     LSGEPYITHPLAVARIMTQLNGDTASVAAALLHDVLEDCDVTHDALAAEFGAELADLV
                     SGVTKLSRIQFSSRQEAQASSFRRLLFAMARDIRVVVIKLADRLHNMRTLSVFGEQKR
                     QDIATETLQIFAPLAERLGIWRVKWELEDLCLREIDPEAYRTIEEKIARRRQERLADV
                     ENAVSALRPRFEAAGIRAEIYGRAKHFYSIYLKMLRQKLQFEEIYDLTALRVIVDTIP
                     ECYVALGLVHQAWAPMPERFTDFIARPKANGYRSIHTKVMGPRNEPLEVQIRTWDMHH
                     ICEYGVAAHWRYKAEAASHTEFDRRISWLRQLLDLGSDLRDANEFLHSLKEGLLRQEA
                     FVFTPKGDLVELPAGSTPVDFAYRIHSEVGHRCVGARVNHRMVPLSTPLQTGDIVEIL
                     TQRGATPSLDWLRFVR"
BASE COUNT          345 a          631 c          628 g          322 t
ORIGIN      
        1 tcagccaaac taaaagtgta aaattccgac tatatgcacc tgaaggattg gatgctgcgg
       61 gccatgctgg cgaccgcctg agcgacgggc tcgaccggtt ccacggttcc aatgcagccg
      121 cgcaggtggc cctcggcagt ccggagtgtc acaaagacgg cggcacgctc cgaagggatg
      181 ctcggatccc ggggaggggc gtagggctcc cccgaagccg cgcaatgcac ggacgctctg
      241 gcaatgcgga ggagagcctg ccgctcctcg agcgagtaag ccatagaacg ccgtccgctg
      301 gggccgcagg ctgagccacg ctgggcctaa cccgtggcgg cttgggaagc agacgacggc
      361 cggcttcgct tgcgccgacc gagtcgggtg ctattatagg gacggctgat tctgcatgtc
      421 aacgcagggc gtgtggcgcg gggccgaagc gcacagatgt gcgaacgctt ccggtgccga
      481 gccagcacag ggggacgacg agttcccgcg ggaggtgggt gatgcggcgt ggtgacggaa
      541 cccgatagcg gcttcgagcc catcgcatcc gcgctggtcg cctaccaccc cgaggccgac
      601 ctggacctga tccgccgcgc ttaccactat gcagtgagaa gccacgaagg gcagacccgg
      661 ctctccggcg aaccctacat cacccatccc ctggccgtgg ctcgcatcat gacccagctc
      721 aacggcgaca cggccagtgt tgcggccgcc ctgcttcatg acgttctcga ggactgcgac
      781 gtcacccacg acgcgctggc cgccgagttc ggggccgagc tggccgacct ggtatccggc
      841 gtcaccaagc tctcgcggat tcagttctcc tcacgccagg aggcccaggc cagcagtttc
      901 cgacgcctgc tgttcgccat ggctcgcgac atccgcgtgg tcgtcatcaa gctggcggat
      961 cgtcttcaca acatgcgaac gctctcggtg tttggtgagc agaagcgcca agacattgcg
     1021 acggagacac tgcagatctt cgcgcccctg gcggagcgcc tggggatctg gcgggtgaag
     1081 tgggagctgg aggacctgtg cctgagggag atcgaccccg aggcctacag gacgatagag
     1141 gagaagatcg cgcgccgtcg ccaggagcgg ctggccgatg tcgagaacgc cgtctcggct
     1201 cttaggcccc ggtttgaggc cgccggcata cgggccgaga tctacggccg tgccaagcat
     1261 ttctattcca tctacctgaa gatgctccgg cagaagctcc agttcgagga gatctacgac
     1321 ctgacggcgc tgcgcgtcat cgtcgacacc atccccgagt gctatgtggc gctggggctg
     1381 gtgcaccagg catgggctcc catgcccgag cgcttcaccg acttcatcgc gcgccccaag
     1441 gcaaacggct accggtccat ccacacgaag gtgatgggcc cccgcaacga gccgctggag
     1501 gttcagatcc gcacgtggga tatgcaccac atctgtgagt acggcgtggc agcccactgg
     1561 cggtacaagg ccgaagcggc cagccacacc gagtttgatc gccgcatctc gtggctccgg
     1621 cagcttctcg acctcggttc ggacttgcgc gatgccaacg agttccttca ctcgctcaag
     1681 gagggcctgc tccgccagga ggcctttgtc ttcactccca aaggcgacct ggtcgagctc
     1741 ccggcgggat cgacgccggt ggacttcgcc taccggatcc actccgaggt gggccatcgg
     1801 tgcgtggggg cgcgagtgaa ccatcgaatg gtgcctctct cgacgcccct ccagaccggt
     1861 gacatcgtcg agatcctcac ccagcgggga gcgacgccca gtctcgattg gctccgcttc
     1921 gtccgg
//
LOCUS       DAOSPL010001017         1920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_59911_length_1920_cov_3.986059, whole genome shotgun sequence.
ACCESSION   DAOSPL010001017 DAOSPL010000000
VERSION     DAOSPL010001017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59911_length_1920_cov_3.986059"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..>1920)
                     /locus_tag="PLQ54_16325"
     CDS             complement(114..>1920)
                     /locus_tag="PLQ54_16325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOS94885.1"
                     /translation="CTEAVGFYHRLRWAPWLKDRETGEPIELSRQDVANGYVNFQGRR
                     VAFTPEEVIEGSIGVTTIDLLETMKRLGRDLAMYPLWAGDMTEFQNIGIQPDDMGMMP
                     FPAAHAGQRPVLQASNSFFMIGKDVLHRGGPTEKDRKAYRDLVWELMWRICSVEGSDE
                     EIRRKVAAGQARFLNPRDLERLGFTDYIREIPPENLRMWREVEQGTILEVVEPFMGKW
                     LQFRDFYQREIIDLVLRPSGKEFDYRSALQTLERDANTGIMFERPPEVLNAYRPRARI
                     IAGLIALFMAFFSVLIVRDQFRRTESQAGVYKGWLPWAMLAPAVVSIALWGYYPLARG
                     LVMAFQDYKVVGHSPFVGLDNFISIALDPNFYHYLITTFRFVLWNLGLAFCTPIFLAF
                     LLTEVPKLKVFFRTLFFLPQMTSGLVVTLMWKEMYAPTAQGTMNRLLAPILSWWHLKP
                     VDWLGNPHTVMACVIIPSIWAAAGISSLIYLAALKSVPEELYEAANIDGATILSRIRH
                     ITIPTIAPLVLINFVGAFIATFQSMGSIFLLTFGGPGKATMVMGMAIWQEAYVNMRFS
                     LATSYAWILGSILIGFTYLQLRILRRVDFRQAKGD"
BASE COUNT          364 a          609 c          608 g          339 t
ORIGIN      
        1 aggaacggat acaccatcgt cagcccgccg atgatcagcg ccacgtagat gcccaaggtt
       61 accgccttac ccttggtcga ccggcgatcg atgtcgctga tgatcggcat gcctcagtcc
      121 cctttcgcct ggcggaagtc cacccgccgg aggatgcgga gttgcagata cgtgaagccg
      181 atgaggatgc tgccgaggat ccaggcatat gaggtcgcga ggctgaagcg catgttcaca
      241 tacgcctcct gccagatcgc catgcccatg accatcgtcg ccttgccggg gccgccaaag
      301 gtgagcagga agatggatcc catcgactgg aatgtggcga tgaacgcccc tacgaagttg
      361 atgagcacca gcggcgcgat ggtggggatg gtgatgtgcc gaatgcgaga gaggatggtc
      421 gcaccgtcga tgttggcggc ctcgtagagc tcctcgggca cggacttcag cgccgccagg
      481 tagatcaggc tgctgatccc cgcggccgcc cagatgctcg ggatgatgac acaggccatc
      541 accgtgtgcg ggttccccag ccagtccacg ggcttgagat gccaccacga aaggatcggc
      601 gccaggagcc ggttcatggt gccctgggcc gtgggggcgt acatctcctt ccacatcagg
      661 gtgaccacca gccctgaggt catctgcggc agaaagaaca gcgtgcggaa gaagaccttc
      721 agcttcggta cctcggtcag gagaaacgcc aggaagatgg gcgtgcagaa cgccagcccc
      781 aggttccaca gcacgaatcg gaatgtagtg atcaagtagt ggtagaagtt ggggtccagc
      841 gcgatgctga tgaagttatc gaggccgacg aagggcgagt gccccacgac cttgtagtcc
      901 tggaaggcca tcaccagccc tcgcgccagc gggtaatagc cccacagcgc gatggacacc
      961 actgcgggcg ctagcatggc ccaggggagc caccccttgt agacccccgc ctggctctcg
     1021 gtcctccgga actggtcgcg gacgatgagc acggagaaga atgccatgaa cagagcgatg
     1081 aggccggcga tgatcctcgc cctcggccgg taggcattga gcacctccgg cggacgctcg
     1141 aacatgatgc ccgtgttggc gtcgcgctcc agagtctgga gcgccgaccg atagtcaaac
     1201 tccttgccgc tcgggcgcag caccaggtcg atgatctcac gctggtagaa gtcgcggaac
     1261 tggagccact tgcccatgaa gggctcgacg acctcgagga tggttccttg ctcgacctcg
     1321 cgccacatcc gtaggttctc gggcgggatc tcgcgtatgt agtctgtgaa tcccaaccgc
     1381 tccaggtcgc gggggttaag gaaacgcgcc tggcccgccg ccaccttccg gcggatctcc
     1441 tcatcggagc cctcgacact gcagatgcgc cacatgagct cccataccag gtcgcggtac
     1501 gccttccggt ccttttccgt tggtccgcca cggtggagca cgtctttgcc gatcatgaag
     1561 aacgagttcg acgcctggag taccggtcgc tggccggcgt gggctgccgg gaagggcatc
     1621 atccccatgt cgtcgggctg gatcccgatg ttctggaact ccgtcatgtc gcccgcccac
     1681 agcgggtaca tggccaggtc tcgacccagc cgcttcatcg tctccagcag gtcgatggtc
     1741 gtcacgccga tactgccctc gatgacctcc tccggcgtga aggccacgcg ccgtccctgg
     1801 aagttcacgt atccgttcgc cacgtcctgg cggctcagct cgatgggctc gccggtctcc
     1861 cgatccttga gccatggcgc ccagcgaagc cggtggtaga agccgacggc ctcggtgcac
//
LOCUS       DAOSPL010001018         1915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_60108_length_1915_cov_3.575269, whole genome shotgun sequence.
ACCESSION   DAOSPL010001018 DAOSPL010000000
VERSION     DAOSPL010001018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60108_length_1915_cov_3.575269"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(688..1473)
                     /locus_tag="PLQ54_16330"
     CDS             complement(688..1473)
                     /locus_tag="PLQ54_16330"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOS94886.1"
                     /translation="MTIDSHTHVFPEHVAERAMESLSARYGARPVGRASPSGLLEHMD
                     ACGVERAVLAPVATRPGQVASINAWLAEIRSDRLVPLGALHPHLPDPANEIQRLLDAG
                     FRGVKLHCHFQGFAMDDPALDRLLELVGDQLVVLMHCGDEIAPLEHVEPTPPRILAMH
                     QRHPEVQLILAHLGGYQEWDEAEALLCGRDVYLDISYVFGHCSDEQVKRMVRAHGADR
                     IIWGSDFPWQSECDALAGLDRLGLSPAERQAVLCGNARRVLGL"
     gene            complement(1604..>1915)
                     /locus_tag="PLQ54_16335"
     CDS             complement(1604..>1915)
                     /locus_tag="PLQ54_16335"
                     /inference="COORDINATES: protein motif:HMM:NF028184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH36 C-terminal domain-containing protein"
                     /protein_id="HOS94887.1"
                     /translation="QIEGDFYPLTPHTTDDAHWIAWQFHRPETGEGLLQVFRRPNSIY
                     EAASLPLRGVEPDVEYRVRVLGEEAERAMLGEEMRQAGVPVSIDTRPGVANIVYEPAK
                     R"
BASE COUNT          286 a          685 c          667 g          277 t
ORIGIN      
        1 catgcggcgc ggcggctcga gttggtcatc gtatgcctcc cggtgctcac tgaaagcgct
       61 tgccctgcag ggtagggccg acaagcgcac ttcgttagca ccatggccct gccctgccgc
      121 acgacaccca gcttcccaac cacggctgcg gacgcggaaa cccagcccac tcgccaaccc
      181 ctcggccgtc acctcctcac ggcgcgccat ctcgtcgagg tcttcggtca gatgcggagg
      241 tgcggagacc ccggaggtcg ccgcgatcga gcgaggggcg caggggcgcc cgccgtcgcg
      301 gcgtcgcccg gccagcaggg ccgtggtgag cgggcagggc atcaccgttc cctcggcgca
      361 ggcggcagca gggctcgggg cactgacggt aaccaaggcc agggcggtat gcgcaaagcc
      421 cgtgcaccgc actgccctgt ctggtggtgg gggcgcaccg ggttgcgccc gaatggcgcg
      481 tgggtctagc gcctcgtcca acacctcata gccgaccggg acggtcggcc tgcggccgac
      541 ggctgaccag ggcacactgg gggcttgcca cgcccgcgtg gttcccccca cggcgcccgt
      601 ctgcaaacag gtccatggac cgatggggcc ccaagtcgcc gccaataggg tccagggtca
      661 ctctccccgg cccgcccgac gggtgtgcta cagccccagc acgcgccgtg cgtttccgca
      721 cagcacggcc tggcgctccg cggggctcag ccctagccga tcgaggccgg ccagcgcatc
      781 gcactcgctc tgccacggga agtcactgcc ccagatgatc cggtcggcac cgtgggcgcg
      841 gaccatgcgc ttgacctgct cgtcggagca gtggccgaac acgtaggata tgtcgaggta
      901 gacgtcccgc ccgcacagga gcgcctcggc ctcatcccac tcctggtagc ccccaagatg
      961 ggcgagaatg agctgcacct cggggtggcg ctgatgcatc gccaggatcc gcggcggcgt
     1021 gggctccacg tgctccagag gcgcgatctc gtccccgcag tgcatcagca cgaccagctg
     1081 atcgccaacc agctccaata gccgatccag agccgggtcg tccatggcaa acccttggaa
     1141 gtggcagtgc agcttcacgc cgcggaagcc ggcatcgagc agccgctgga tctcgttggc
     1201 cggatcgggc aggtgggggt gcagcgcccc caggggcacg agccggtcac tgcggatctc
     1261 ggcgagccat gcgttgatcg acgcgacctg gccggggcgc gtggctacgg gggccagcac
     1321 ggcgcgctcg accccgcacg cgtccatgtg ctcgagaagg ccactggggc tggctcgccc
     1381 cacaggccgc gcgccgtacc gcgccgacag cgactccatg gcgcgttcgg ccacatgctc
     1441 ggggaatacg tgggtgtggc tgtcgatggt caccgattgt cacctcgcag gcacctctcg
     1501 gtcgcgaaca ccgggccgac caggttgggt cagctggggg caggtgggac cctgctcggc
     1561 ccgaagcgcc ccgggtccca cctgcccgac gcgcccggcg ccgctaccgc ttggcaggct
     1621 cgtagacgat gttagccacg ccggggcgcg tgtcgatcga gacgggaacg ccggcctggc
     1681 gcatctcctc gcccagcata gcccgctcgg cctcttcgcc aagcacgcgt acgcggtact
     1741 cgacgtcggg ctcgacgccg cgcaggggga gggaggcggc ctcgtagatg ctgttcggtc
     1801 tccggaacac ctgcaggagc ccctcgcccg tctctggccg gtggaactgc caggcgatcc
     1861 agtgggcgtc gtcggtggtg tggggcgtca gcgggtagaa gtcgccctcg atctg
//
LOCUS       DAOSPL010001019         1914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_60162_length_1914_cov_2.926842, whole genome shotgun sequence.
ACCESSION   DAOSPL010001019 DAOSPL010000000
VERSION     DAOSPL010001019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60162_length_1914_cov_2.926842"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLQ54_16340"
     CDS             <1..408
                     /locus_tag="PLQ54_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOS94888.1"
                     /translation="PMDQQTLFTAGTTGLAYLLKERVGHREELVRAIEAVHTGGSVID
                     PAIVDALLHARQHTASSPLRHLSPRELEVLAAMAQGLTNRAIADRLYVSESAVSKHVG
                     AIFHKLALEEGTGLDRRVSAALAYLSSSGPNHR"
     gene            complement(490..756)
                     /locus_tag="PLQ54_16345"
     CDS             complement(490..756)
                     /locus_tag="PLQ54_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008729882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HOS94889.1"
                     /translation="MSNWRLVYSRQAQKDAKKLASSGLKSKAQRLLDVIADDPFANPP
                     RYEKLVGDLAGCYSRRINIQHRLVYEVLPDDGVVHVLRMWTHYE"
     gene            complement(753..995)
                     /locus_tag="PLQ54_16350"
     CDS             complement(753..995)
                     /locus_tag="PLQ54_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004014244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOS94890.1"
                     /translation="MTAVSATSARANLYRLIDQVNDESEPLTITGQRGNAVLIGEEDW
                     QAIQETLFLASVPGLSESIRQARAEGIDAGSSELDW"
     gene            complement(1128..1682)
                     /locus_tag="PLQ54_16355"
     CDS             complement(1128..1682)
                     /locus_tag="PLQ54_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94891.1"
                     /translation="MRPIVPSVEEVAQLVADLVVSEWPRTEGERAVWFERHGIVTEGA
                     ELIRDEHGSESWISRDPGAPWPAVGWEVCKAEFVGFNWFLWRGLPEDVMPALAEELRA
                     RFVEIAGEPMDEKREEGDDYRFTAYWQRGGRSIDMYLHGGPVLEGRFHDQPVVQLSVD
                     HVQRSRKAAEAAAGARVAEGRARP"
BASE COUNT          333 a          705 c          561 g          315 t
ORIGIN      
        1 ccaatggacc agcagacact cttcaccgcc ggcaccaccg gcctggccta cctgctcaaa
       61 gaacgcgtcg gccaccgcga agagctcgtc cgcgctatcg aggccgtgca cacgggtggc
      121 tcggtcatcg accccgccat cgtcgacgct ctcctgcacg cccgacagca caccgcatca
      181 tccccgctgc ggcacctcag cccacgcgag ctcgaggtgc tcgccgccat ggcccaaggc
      241 ctcaccaacc gcgccatagc cgaccgccta tacgtctcag agtccgctgt cagcaaacac
      301 gtcggcgcca tcttccacaa gctcgcactc gaagaaggca ccggtctcga tcgacgagtc
      361 agcgccgccc tcgcctacct atcctcaagc ggcccgaacc accgatgaag gtgccccact
      421 tcgccgggaa gcgcccgcgt ccatgggtcg atgttggcgt cggagggtac ctattggacc
      481 gcgtgagagc tactcgtagt gggtccacat gcgcaggacg tgtacgacgc cgtcgtcggg
      541 aaggacctcg tagaccaggc ggtgctggat gttgatgcgc cgggagtagc agccggccag
      601 gtcgccgacc agcttctcgt atcggggtgg gttggcaaag gggtcgtcag cgatcacgtc
      661 aaggagccgc tgggccttgg attttaagcc agacgaagca agcttcttgg cgtccttctg
      721 cgcttggcgg gagtagacaa gcctccagtt gctcaccagt cgagctcgct ggagccggcg
      781 tcgatccctt cggcgcgcgc ctggcggatc gactcactca aacccggcac tgaggccagg
      841 aagagcgtct cctgtatggc ttgccagtcc tcttcaccga tgagcaccgc gttgccgcgc
      901 tggccggtaa tggtcagcgg ctcggactcg tcgttgacct ggtcgatcag ccggtagagg
      961 ttcgctcgtg ctgacgttgc acttactgcg gtcatgacat ctacctccac cccaaccgta
     1021 cgtcattgcg tacggtcaga caagtgtgcc agcggttcct cacaaccacg cacaggcaga
     1081 gcgcccacga agctgcgtca tgccgctgcc aatcggcgca tccgctgtca cggacgcgcg
     1141 cgtccttccg ccacccgcgc ccctgccgcc gcctcggctg ccttccggga gcgctggacg
     1201 tggtcgacgc tcagctgcac caccggctga tcgtggaaac gcccctccag cacgggtcca
     1261 ccgtggaggt acatgtcgat agagcgaccg cctctctgcc agtaggcggt gaagcggtag
     1321 tcgtccccct cctctcgctt ctcgtccatg ggctcgcccg cgatctcgac gaaccgggca
     1381 cgcagctcct ccgcaagcgc aggcatcacg tcctccggca gcccgcgcca caggaaccag
     1441 ttgaacccga cgaactcagc cttacagacc tcccagccca cggccggcca cggggccccg
     1501 gggtcacggc tgatccagga ctcgctgccg tgctcgtccc ggatcagctc ggcaccctcc
     1561 gtcacgatgc cgtgccgctc gaaccacacg gcccgctcgc cctcggtcct cggccactcc
     1621 gagacgacca gatccgccac gagctgagcg acctcttcga ccgagggaac gatcggccgc
     1681 aagtcatcac tcatgaccgg cagcgtagtt ccgcgcgcag acaggcaccc gcagggccaa
     1741 gtccggcatg aacgccggac cgccaggacg agtacacggt cactgccgcc cccaggatct
     1801 cgcctgcgac cgggtcgggc caggccctca agacgctctc ggccgtcatt ccccgacgtc
     1861 cgctcatcgg ctacttgttt cattgacggc atggccggta tctgacgagg ctcg
//
LOCUS       DAOSPL010001020         1913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_60223_length_1913_cov_2.094187, whole genome shotgun sequence.
ACCESSION   DAOSPL010001020 DAOSPL010000000
VERSION     DAOSPL010001020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60223_length_1913_cov_2.094187"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /gene="nrdD"
                     /locus_tag="PLQ54_16360"
     CDS             complement(<1..999)
                     /gene="nrdD"
                     /locus_tag="PLQ54_16360"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOS94892.1"
                     /translation="MGVESVRVLGVEKRNGQIVPFDQSKITAALLKAGRATGEFDLAT
                     AQTLSDKVAAVLEWQHSGETVHVERIQDLVEDTILDARYIRTAKAYIIYRDQHAKMRD
                     LADALDPTHLIGSYLDLRDWRVNENSNMSYSLQGLNNHIAASVTARYWLHKVYPAEVR
                     EAHESGDLHLHDLQLLSIYCCGWDLRDLLLRGFRGADGKIESAPPRHLRTALGQIVNF
                     FYTLQGEAAGAQAFANFDTYLAPFVRADGLEYSEVKQAMQEFIFNMNVPTRVGFQTPF
                     TNLTMDLTPTGEIADEAVVVGGATLDATYSEYQAEMDIINRAFAEVMLEGDARGRVF"
     gene            1620..>1913
                     /locus_tag="PLQ54_16365"
     CDS             1620..>1913
                     /locus_tag="PLQ54_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94893.1"
                     /translation="MRLMPLALLALILGIMVSAGCKSDEDSTEPVTVSGIGSGEKGTR
                     ADATGGPSSDQDAGEVEPAGTPEVEGEAEEAGATEKEAGKAPGAPGGKSPTDGL"
BASE COUNT          319 a          612 c          642 g          340 t
ORIGIN      
        1 gaacacgcgt ccccgagcat cgccctcaag catgacctcg gcgaaggccc gattgataat
       61 gtccatctcg gcctggtact cgctgtaggt cgcatcgagc gtcgctccgc ccaccaccac
      121 cgcctcgtcg gcgatctcgc ctgtgggggt cagatccatc gtcaggttcg tgaagggcgt
      181 ctggaacccc acccgggtag gcacgttcat gttgaaaatg aactcctgca tcgcctgctt
      241 gacctcggag tactcgaggc catcggcgcg gacgaaggga gccaggtagg tgtcgaagtt
      301 ggcgaatgcc tgggcaccgg ccgcctcgcc ctgcagggtg tagaagaagt tcacgatctg
      361 cccgagagcc gtacggaggt gacgcggcgg ggcgctctcg atcttcccat cggcgccgcg
      421 gaacccacgg agaagcagat cccgcagatc ccatccgcag cagtagatgg agagaagctg
      481 caggtcgtgc aggtgcaggt cgccgctctc gtgggcctcg cgcacctccg cgggatagac
      541 cttgtgcagc cagtaccgcg ccgtgaccga agccgcgatg tggttgttga gcccctgcaa
      601 ggagtagctc atgttgctgt tctcattgac ccgccagtcg cgcagatcca ggtaactgcc
      661 aatgaggtgg gttgggtcca gggcatccgc cagatcgcgc atcttggcgt gctggtcgcg
      721 gtagatgatg tatgccttgg ctgtgcggat ataccgggca tcgaggatcg tgtcctcgac
      781 caggtcctgg atccgctcga cgtgcaccgt ctcccccgag tgctgccact cgagcacggc
      841 cgcaaccttg tcggaaagcg tctgggccgt tgcgaggtca aactctccgg tggcgcggcc
      901 cgccttcagc aaggctgcag tgattttcga ctggtcgaag ggcacgatct gcccgttgcg
      961 cttctccacg ccgaggactc gcacgctctc gactcccatt cgcgaggtct ccatgcgggt
     1021 gccaggagcc ccttgggggt tctccccagc accgtcggga tggaatcccg gtgaccaacg
     1081 ctctcgcttt acgggcccag tattgaaggg tccttctcgc tgtttttgcc ttgcacgtgc
     1141 cacatgggcc gatcgggacc acagtgtccc ggaagacctg gtgccacaac tgacagtggc
     1201 actcgcttgt tcgccacaat atgtagtatg ttttcgcgcc cagcgcaagc catggcccac
     1261 ccgcttccgg cccagagccg atcggcggac cgggtgggcc gcatggccga cggatcgcac
     1321 ccgaacgcgc cttcacgccg cactcagggg ccgggaatgg gcgcgttgga ctcccccacg
     1381 tctgacccgc gtggttcgcc ccgtggcggc cctccgcaac caggcgcttg ggcccttgtc
     1441 gcgaaccagt accgggctat tcgggtaggc aacacagcca tccgcgccag agggaccccc
     1501 cggcctgtac gcgaactgcc ctgcgtctgc ttcgtgcggg gcgcgcgatt gtttgaaccc
     1561 gccggggcgg tgtgcgtctt ttgccgtggg cccccgtggc cccatgggag gaatgggcga
     1621 tgcggctcat gccgttggca ctgcttgcac tgattctggg catcatggtg agtgccggat
     1681 gtaagagcga cgaggactcg acggaaccgg tgacggtctc gggcatcggg tcgggagaga
     1741 agggcacgcg ggctgacgca accggtggcc cgtcgtcaga ccaggacgcg ggtgaggtcg
     1801 agccagcggg gacgccggag gtagaggggg aagccgagga ggccggcgcg acggaaaagg
     1861 aggccggcaa ggcacctggc gctccgggcg gcaagagccc caccgatggc ctg
//
LOCUS       DAOSPL010001021         1912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_60250_length_1912_cov_3.088853, whole genome shotgun sequence.
ACCESSION   DAOSPL010001021 DAOSPL010000000
VERSION     DAOSPL010001021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60250_length_1912_cov_3.088853"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1912)
                     /locus_tag="PLQ54_16370"
     CDS             complement(<1..>1912)
                     /locus_tag="PLQ54_16370"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOS94894.1"
                     /translation="VLKPEQLEADMAAILALGVFNPDPERTRVNVTEVEGGVKIVFQL
                     TENPRLTAVMFEGNQIITTDQLKAAVGDLLVEGEIYNSVLANRAVDRIIEAYTSAGYE
                     AIVSSGRIDEQGRLVLTIVEARIGSLALHVAPPVTVNTEALQAWLAVQPGTIFSRSLV
                     SSLARRLRDSGLFAQVDIPPPVVGEDISKITLAIQGTCLAWPHPNRETVGFVDVQKAV
                     DAVCTAKPVPAENWALELPLAAEDIERAEEAFTRAPTDLNAALNLAVSYFRASPGARA
                     ASAAGRAADSCAASITPESPPADRAAYARLLVLAQRWDQAYEVAAPLLDAPDPTVRGL
                     AVLVEARVCRLAQHAAALAAGAPGAEPVPDGAPLEWAVEYLNASPDPTKLLATEPGHD
                     YVRAVTAALRYLRGLDPAGVCAQLDEFRRLAFLLNALNPLNSYAGFDTEPRTPITTAL
                     ADPRVMAGLRTRSANPEDRAARYGLARLLMIHAFAATLSGSQGASSLGFADADAVKAA
                     AAEAGQIFQQLLKENPVAMAPVRQDIGLCLILQGKAPEGRQWLYQSVADGKTRQAIGV
                     IYDTTRPDVFLTGEGAPTPDEARAAILKEVREFCATHQPLPPSLVELLVTVAVWNSDW
                     ATADQAIAPLVAP"
BASE COUNT          296 a          651 c          671 g          294 t
ORIGIN      
        1 cgggcgccac gagtggcgcg atggcctggt ctgccgtagc ccagtcgctg ttccagacgg
       61 ccacggtgac gagcagctct accaggctgg gcggcaaggg ctggtgcgtt gcgcagaact
      121 cccgcacctc cttgaggatg gccgcacgcg cctcgtccgg tgtcggggca ccctcgccgg
      181 tcaggaacac atctggccgc gtcgtgtcat agatgacgcc gatcgcctgc cgggtcttgc
      241 cgtccgccac cgactggtac agccactgac gcccctccgg tgctttgccc tgtaggatga
      301 ggcacagccc gatgtcctgg cgcacgggcg ccatggccac cgggttctcc ttgagcagtt
      361 gctggaagat ctggcccgcc tcggcggcgg ccgccttgac ggcatccgcg tccgcaaagc
      421 ccagcgagct ggcgccctgg gagcccgaca gagtcgcagc gaacgcatgg atcatcagaa
      481 gccgggcgag cccgtaccgt gccgcccggt cttcagggtt ggcgctgcga gtccgcaagc
      541 cggccatgac ccgcggatcg gccagtgccg tggtgatcgg agtccgcggc tcggtatcga
      601 agcccgcata cgagttgagg gggttgaggg cgttgagcaa gaaggccaac cgtctgaact
      661 cgtcgagctg ggcgcacacg ccggcggggt ccaggccgcg aaggtaccgc agcgctgccg
      721 tgactgcgcg gacgtagtcg tggccgggct cggtggccaa gagcttcgtt gggtcaggcg
      781 acgcgttcag gtactccacg gcccactcga gcggcgcccc atcgggcacc ggctcagcgc
      841 ccggcgcacc ggccgccagt gcagccgcgt gttgggccag gcggcacaca cgggcctcca
      901 cgagcacggc caggccccgc acagttgggt cgggtgcgtc gagcagcggc gcggccacct
      961 cataggcctg gtcccaacgc tgggcgagga caagcagtcg cgcataggcc gcacgatcgg
     1021 ccggcgggct ctcgggcgtg atcgacgccg cgcacgagtc ggccgcacga ccggccgccg
     1081 aggcggcccg ggccccgggg gaggctcgga agtaggatac cgccaagttc agcgccgcat
     1141 tgagatcggt cggcgcccgg gtgaacgcct cctccgctcg ctcgatgtcc tccgcggcca
     1201 atggcagctc cagcgcccag ttctcggccg gaaccggctt ggcggtgcat acggcatcca
     1261 cggccttctg gacgtcgacg aacccgacgg tctcccggtt gggatgcggc caggccaggc
     1321 acgtgccctg gatcgccagg gtgatcttcg agatgtcctc gcccaccacg gggggaggga
     1381 tatccacctg ggcgaacaac cctgagtccc gcagtcgccg cgccagactg gagaccagcg
     1441 atcggctgaa gatggtgcca ggctggaccg ccaaccaggc ctggagggcc tcggtgttga
     1501 ccgtcacggg cggagcgaca tggagtgcca gtgacccgat acgggcctcg acgatggtca
     1561 gaaccaaccg gccctgctca tcgatgcgcc cgctggacac gatggcctca tatcccgcgc
     1621 tggtgtaggc ttcgatgatg cgatcgaccg cgcggttggc gagcaccgag ttgtagatct
     1681 cgccctccac gagaaggtcg cccacggctg ccttcagctg gtctgtcgtg atgatctggt
     1741 tgccttcgaa catcacggcc gtgaggcggg ggttctcggt gagctggaag acgatcttca
     1801 cgccgccctc gacctcggtc acattcacgc gcgtccgctc cgggtcgggg ttgaacacgc
     1861 cgagggccag gatggccgcc atgtcggcct cgagctgctc gggcttcagg ac
//
LOCUS       DAOSPL010001022         1910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_60335_length_1910_cov_3.823181, whole genome shotgun sequence.
ACCESSION   DAOSPL010001022 DAOSPL010000000
VERSION     DAOSPL010001022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60335_length_1910_cov_3.823181"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            317..>1910
                     /locus_tag="PLQ54_16375"
     CDS             317..>1910
                     /locus_tag="PLQ54_16375"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOS94895.1"
                     /translation="MPERPKRPLGDITVHCIGNAHIDAAWLWRWDETVRVVRETFESA
                     LDRMDETPEFIFTCSQVVYFRWMEEQYPELFSRIRERVEEGRWDLAGGWWVQPDCNIP
                     SGESLVRQALYGQRYLDSRFGKMATVGYNPDTFGHCNAIPQLLAKSGMNAYLFFRPGP
                     HEKTLEGETFWWESPDGSRVLASRPPHHYCTDEEELAERLRVAAEQAPAPLTDVMCFY
                     GVGDHGGGPTRRAIASILAHAETPGGPRARFARIEDFYREAASHGAALPVLRDDLQHH
                     APGCYAVHSEVKRRNRECEQLLSAAESMATLAWAAGGPDYPDGELTQAWAAVLFNQFH
                     DILAGTSIPEVYEDANEMYDEAEALAQEALEPALTSLAARADTRGDGLPLVVFNPLSW
                     AHEVPVEVEIPWDYRLDLLSVWGPDGRQVPVQIDRVQGPATAGMLRIVFLARVGPMGH
                     AVYHCRPVMPEAFPPLTADGCRIENDRLRVVVDGETGRLTSVFDKANGVEVLSQPAAD
                     FLVLADRSDTWSHGVAAFRDEVG"
BASE COUNT          284 a          654 c          678 g          294 t
ORIGIN      
        1 gcacggcgtc tcccactgcc cccggggcga gaaccgcggc gggccgggta gggcgatggc
       61 agggctgatc ctgccggtgc tcaccatcgt ggcgacggcc atcgtgggcg tcgtcgtcgg
      121 gcggtagggg gcggccgcag gcgccgacgg ccccgggacc ctgctcgccg agatccttcg
      181 ctccgcagat gctccgctca ggatggcacc aacggcaacg cgcgccgtgc gcccccgagc
      241 caccccgcgc gttgacttcg ccctccgcat gggtcatcgt tcgcgtactg cgctggaccg
      301 acggaggaca tcgatcatgc ctgagagacc caaacgcccg ctgggcgata tcacggtgca
      361 ctgcatcggc aacgcccaca tcgacgccgc gtggctgtgg cgttgggatg agaccgtccg
      421 cgtggtccgc gagaccttcg agtcggctct cgaccgcatg gatgagacac ccgagttcat
      481 cttcacctgc agccaggtgg tgtacttccg ctggatggag gagcagtacc ccgagctgtt
      541 ctcgcggatc cgcgagcggg tcgaggaggg ccggtgggat ctggcgggcg gctggtgggt
      601 ccagcccgac tgcaacattc cgtccggtga gtcgctggtg cgccaggcgc tctacggcca
      661 gcgctacctc gacagccggt tcgggaagat ggccacggtc ggctacaacc cggacacctt
      721 cgggcactgc aacgcgatcc cgcagcttct ggccaagtcc ggcatgaacg cgtacctctt
      781 cttccgcccc gggccccacg agaagacgct cgagggcgag accttctggt gggagtctcc
      841 cgacggctcg agggtgctgg cctcgcggcc accccaccat tactgcacgg acgaggagga
      901 gctggccgag cggttgcgtg tcgctgcgga gcaggcaccg gccccgctga cagacgtgat
      961 gtgtttctat ggggtcggcg accacggcgg cggaccgacc cggcgcgcca tcgcctcgat
     1021 cctcgcccac gcggagacgc ccggcggccc gcgcgcgcgc ttcgcccgca tcgaggactt
     1081 ctaccgcgag gcggcctccc acggcgcggc gctacccgtg ctgcgcgatg acctccagca
     1141 ccatgcgccc ggatgctacg cggtgcattc cgaggtgaag cgccgaaacc gcgagtgtga
     1201 gcagctcctt tcggcggccg agtcgatggc cacactcgca tgggccgccg gcgggccgga
     1261 ctacccggat ggcgagctga cccaggcgtg ggcggcggtg ctgttcaacc agttccacga
     1321 cattctggcg ggaaccagca tcccggaggt ctacgaggac gccaacgaga tgtacgacga
     1381 ggccgaggcg ctggcccagg aggcgctgga gcccgccctg acgtcgctgg cggctcgggc
     1441 cgacacgcgc ggcgatggct tgcctctggt cgtgttcaac ccgctgagct gggcgcacga
     1501 agtgccggtt gaggtcgaga tcccgtggga ctaccggctc gaccttctct ccgtgtgggg
     1561 gccagacggg cgccaggtgc cggtccagat cgaccgcgtg cagggcccgg caaccgccgg
     1621 gatgctccgg atcgtgttcc tagccagggt gggcccgatg ggccacgccg tgtaccactg
     1681 caggccggtc atgcccgagg catttccccc gctcacagcc gacgggtgcc ggatcgagaa
     1741 cgaccggctc cgcgtggtgg ttgacgggga gaccggccgg ctgaccagtg tgttcgacaa
     1801 ggccaacggc gttgaggttc tgtcgcaacc ggccgccgac ttcctcgtcc tggctgatag
     1861 gagcgacacg tggagccatg gcgtggccgc gtttcgcgat gaggtgggcc
//
LOCUS       DAOSPL010001023         1909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_60397_length_1909_cov_2.702805, whole genome shotgun sequence.
ACCESSION   DAOSPL010001023 DAOSPL010000000
VERSION     DAOSPL010001023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60397_length_1909_cov_2.702805"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1851)
                     /locus_tag="PLQ54_16380"
     CDS             complement(<1..1851)
                     /locus_tag="PLQ54_16380"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOS94896.1"
                     /translation="MVRRALAVPMVLRAGRSATRTDAVEHQIVTGTWSVPHEGDELTM
                     PDGSTRTWKTVEANADGWFEDDALWGGYAHAAITPPEPGVWILEAQGHSAVYVNGELR
                     VGDPYHNGYVQLPVALHAGANDLLFLVGRGFRASLTRAVSPALLNLADPTMPDLIVGR
                     AADTWGAVVVLNATGETARDLSLVSLAEGGQAVRTPLPPLLPLATRKVGFRIAAPARR
                     EGGTLRLRLALVSGSGAGQRTLDVGRLDLRVRRPAESRKVTFLSAVDGSVQYYGVMPP
                     SGQPGCRGPALFLTLHGAGVEAIGQADAYSPKSWGYLVAPTNRRPYGFDWEDWGRWDA
                     IEVLEEAERTLHTDPSRVYLTGHSMGGHGVWHLGACFPDRFAAIGPSAGWASLMSYGG
                     GGPREGGSPVEKLIRRTSSPSDTPALAPNYAHHGVYVLHGADDDNVPVREARSWVERL
                     TRFHRDFVYHEQPGAGHWWDVSDEPGADCVDWAPMFDFFARHAVPCTAAVRRVQFATF
                     SPSVSADCHWVTVETQLRAFELSTVDIGVDPASRRFVGTTGNVHRLGIRLDALPAAGP
                     VSVELDGCPLTGIDAGGPGSELRFERVGNGWRVCARPPSSMSHPCRCTARR"
BASE COUNT          317 a          688 c          643 g          261 t
ORIGIN      
        1 gcgccgggcc gtgcaacggc aaggatgaga catcgacgac ggcggccggg cgcagacccg
       61 ccagccgttg ccgacgcgct cgaaccggag ttcggagcct ggcccgccgg catcgatgcc
      121 ggtgagagga cacccgtcga gctccaccga gacgggcccc gctgcgggta gcgcatcgag
      181 ccggatgccc agccggtgca cgttgcccgt agtgcccacg aaccgtcggc tggccgggtc
      241 gacgccgatg tccaccgtgc tcaactcgaa tgcccgaagt tgcgtctcga ccgttaccca
      301 gtggcagtcg gcggagacgc tcgggctgaa tgtggcgaac tgcacacgcc gaacggcagc
      361 cgtgcaaggc acggcgtggc gggcgaagaa gtcaaacatg ggcgcccagt ccacgcaatc
      421 ggctcccggc tcatcggaga cgtcccacca gtgccccgca ccgggctgct cgtggtacac
      481 gaagtccctg tggaacctcg tcaaccgctc gacccagctc cgagcctcgc ggacgggcac
      541 gttgtcatca tccgccccgt gcaggacgta gacgccatgg tgtgcgtagt tcggggccag
      601 ggcaggcgtg tcgctcggcg acgacgtccg gcggatcagc ttctcgacgg gcgagccgcc
      661 ctctcgcggg ccgccgccac catacgacat caggctcgcc cagcccgcac tcgggccgat
      721 ggccgcgaaa cgatcgggaa agcatgcacc caggtgccac acgccatggc cgcccatcga
      781 gtgcccggtc aggtacactc gcgaggggtc ggtgtgaagc gtcctctcgg cctcctcgag
      841 cacctcgatg gcgtcccatc gcccccaatc ctcccagtcg aacccatagg gccgccggtt
      901 ggtcggtgcc acgaggtagc cccaggactt gggcgagtag gcatctgcct ggccgatggc
      961 ttcgacgccg gcaccgtgga gggtcagaaa cagcgccgga ccacggcagc caggttgtcc
     1021 cgatggcggc atgactccgt agtactgaac gctcccatct acggcgctga ggaaggtgac
     1081 cttgcgcgac tccgccggcc gacggacgcg caggtcgagc cgcccgacat ccaatgtcct
     1141 ctgtccggcc ccactgccgg acaccagcgc cagccggagc cggagagtgc caccctctcg
     1201 ccgggccggc gcggcgatgc ggaagcccac cttccgcgtg gccaagggca acagcggggg
     1261 aagtggcgtc cgaaccgcct gccctccctc ggccagcgag acgaggctga ggtcgcgggc
     1321 cgtctcgccc gtggcgttca gcaccaccac cgcgccccat gtgtcggccg cgcggccgac
     1381 gatcaggtcc ggcatcgtcg ggtcggcgag gttaagcagc gcgggcgaca cggcgcgggt
     1441 taggctggcg cggaaccccc gccccaccag gaagagcaga tcgttggccc ctgcgtgaag
     1501 cgccacgggc agttggacat agccgttgtg gtaggggtca cctacccgca actcaccgtt
     1561 cacataaacg gcgctatgcc cctgggcctc gaggatccag acgccgggct ccggcggcgt
     1621 gatcgccgca tgggcatatc caccccacag ggcgtcgtcc tcgaaccagc catcggcgtt
     1681 ggcctcaacg gtcttccacg tccgtgttga gccgtccggc atggtgagct cgtcaccctc
     1741 gtgcggcacc gaccacgtgc ccgtgacgat ctgatgctcg acggcatcgg tgcgcgtggc
     1801 tgaccggccg gcgcgcagca ccatggggac agccagggcc ctacggacca cgacctcgtc
     1861 agccggggcg gccgggcacc gctgggggct gcacagcgat accaaagcc
//
LOCUS       DAOSPL010001024         1908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_60420_length_1908_cov_3.658931, whole genome shotgun sequence.
ACCESSION   DAOSPL010001024 DAOSPL010000000
VERSION     DAOSPL010001024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60420_length_1908_cov_3.658931"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="PLQ54_16385"
     CDS             complement(<1..1113)
                     /locus_tag="PLQ54_16385"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase"
                     /protein_id="HOS94897.1"
                     /translation="MDGDLNLSMFRAYDIRTPSAKLTPDLAARLARAEAVYFRHTLGV
                     GGVVLAHDARQTGPAYLSIAAEEFVAAGLEVVVIPCACSTPMLYFAAMQHPEWAAIMI
                     GASHNPPGDTGQKILGPDVFPVARGIGPEGGLDRIQELYEADARPPRSARGRIMAHDP
                     ADAYVQYSLGLAGVRPGGLRGIRVVHDYLYGAAGRDLMLGFARAGADLVPLHFTADGA
                     FPLGAPNPVIPHVTAPGQETLREAGALLGTFFDGDGDRIDFYRGDGAYLSSSFVYAGI
                     LPLIRERFAGPGWVVFADLKCNPLALIRMAATGVSVRVIRNGHSQIKFSMIQEPSAIG
                     TVEESAHFYEAFTLGGRTRYCTENSLYTALLVTRAWA"
     gene            complement(1168..>1908)
                     /locus_tag="PLQ54_16390"
     CDS             complement(1168..>1908)
                     /locus_tag="PLQ54_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94898.1"
                     /translation="HARRSFSILFAQMTEGVRWMPPPGVGWDASSQTVTRDGYRVRLA
                     DGVFSALLPTGLGESILSGLVYSSGQTGWVIEDGEPAEVRVLADGPVRTVVSVHRRLK
                     AGVAYEKRYTFYAHHFVVDTSVQNATTGSLCRAYYAQRGQFEDDRGHRAFIDGHGDGE
                     GVMAANPGARWYAVYTDSWAHSCVALSPTTNLTYWDGGAMGGISLDAADARAHQVAYI
                     IHDGQPDASFAARDYEQLSDPPRAARVF"
BASE COUNT          319 a          626 c          680 g          283 t
ORIGIN      
        1 ggcccaggcc cgagtcacga ggagcgccgt gtagaggctg ttctccgtgc agtaacgagt
       61 gcggccgccg agcgtgaagg cttcgtagaa gtgggccgac tcctccactg tcccgatggc
      121 ggacggctcc tggatcatcg agaacttgat ctgcgagtgt ccgttgcgga tgacccggac
      181 tgacacgccg gtggccgcca tgcggatcag tgccagcggg ttgcacttga gatcggcgaa
      241 cacgacccag cccggcccgg caaaccgctc acggatcagc ggcaatatgc cggcgtacac
      301 gaagctcgac gacagatagg ccccgtcgcc gcggtagaag tcgatgcgat cgccgtcgcc
      361 gtcgaagaaa gtgcccagca gcgcaccggc ctccctcagt gtttcctgac ccggtgcggt
      421 cacgtgcggg atgaccggat tgggggcacc cagtgggaag gccccatcgg cagtgaagtg
      481 gagggggacg agatcggcac ccgcccgcgc gaaacccagc atcaggtcgc gccccgccgc
      541 gccgtagagg tagtcatgga ccacgcgaat cccccggaga ccgcccggtc ggacaccggc
      601 gagtccaagg ctgtactgga cataggcgtc ggctgggtcg tgggccatga tgcggccgcg
      661 ggcactccgc gggggccgcg catcggcctc gtagagctcc tggatgcggt cgagtccgcc
      721 ctcggggccg atgcctcgtg caaccgggaa gacgtcgggc ccgaggatct tctgcccggt
      781 atcgcccggc gggttatgcg aggcgccgat catgatggcc gcccactccg ggtgctgcat
      841 ggccgcgaag tagagcatgg gtgtggagca cgcgcagggt atcacgacga cttcgagccc
      901 ggcggcgacg aactcctcgg cggcgatgct caggtaggcg ggcccggtct gccgcgcgtc
      961 gtgggcgagg acgacgccac caacgccgag agtgtggcgg aagtacacgg cttcggcgcg
     1021 cgcgagccgc gcggccagat cgggcgtcag cttggccgag ggcgtacgga tgtcgtacgc
     1081 ccggaacatg gagaggttca gatcgccgtc catcgaggcc tccggggaag cgtgctccca
     1141 tcatagccga tggccgcacg gcggtgtcta gaacacgcgc gcggccctcg gtggatcgga
     1201 gagctgctca tagtcgcgcg ccgcgaagga tgcgtccggc tggccgtcgt gaatgatgta
     1261 ggccacctgg tgggcccggg cgtcggcagc gtcgagcgag atgccaccca tggctccgcc
     1321 atcccagtac gtcaggttcg tcgtcggact gagggcgacg cacgagtgcg cccacgagtc
     1381 ggtgtagact gcgtaccatc gcgcgccggg gttcgcggcc atcacgccct cgccatcgcc
     1441 gtggccgtcg atgaaggctc gatgccccct gtcatcctca aactgcccgc gttgggcgta
     1501 gtaggcccgg cagagggaac cggtcgtcgc attctgcacc gaagtgtcga cgacaaagtg
     1561 gtgggcgtag aacgtgtagc gcttctcgta tgcgacgccg gccttgagcc tgcgatggac
     1621 cgacacgacc gtgcgcaccg gcccgtccgc aagcacgcgg acttcggcgg gctcgccgtc
     1681 ctcgatcacc caccccgtct ggccggagga gtagacgaga ccggagagga tactctcgcc
     1741 cagacccgtt gggaggagag ccgagaacac gccgtcggcg aggcggacgc ggtagccgtc
     1801 cctggtgaca gtctggctgc tggcgtccca tcccacgccc ggcggcggca tccaccgcac
     1861 gccctcggtc atctgcgcga acaggatgct gaagctgcgg cgcgcgtg
//
LOCUS       DAOSPL010001025         1908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_60430_length_1908_cov_3.016190, whole genome shotgun sequence.
ACCESSION   DAOSPL010001025 DAOSPL010000000
VERSION     DAOSPL010001025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60430_length_1908_cov_3.016190"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="PLQ54_16395"
     CDS             <1..915
                     /locus_tag="PLQ54_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94899.1"
                     /translation="EPIMSPAWSPDGTRLAYVSGGDLWLTCLDGSPNRLVQASALRAS
                     TGFWARKTPLCLDPTWTPDGQAILFLHSTFDGFLNLYRVNADGTGIMLLGETVAGPPA
                     PHPTLPRLAIPYPGGVRLLDLSSLPEKRSIVAVLPKAAEPCWSPDGSRLVVVCDGELW
                     QTNPDGEQARKLFWNDRCPVRQPVWLPDGQTLLVIASVRRTAGPRYDEIWLVPGRPGG
                     GPRVLASGGDQGGGQRAIGFLAWWAPGRQAIYGFGYRPTTFRWVREDGTAGDRASGLI
                     QPSWRPTASVVPASAADSETPQEKQPGT"
     gene            complement(1199..>1908)
                     /locus_tag="PLQ54_16400"
     CDS             complement(1199..>1908)
                     /locus_tag="PLQ54_16400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS94900.1"
                     /translation="SRQGWIELSEVVRRRSLLCVVGADTSFCFGMAVLQVFGPAFLQQ
                     HRGYAVGATPVWELVAAGVISVASGGLGFWCATWIVDRTQPRRPNVSTVCGAAGGAVL
                     APLLLLLILGTSKAAGLLLLAPTAFFMAWQGGLAVVATQALAPVHLTATTLGFLALSN
                     NLVGEASGAWAAGAVSDAQGGSMEAALLLAPAAMLLCSELFLFASRTAGDDVATIHAL
                     EQQAGQSTARAPGDSSD"
BASE COUNT          301 a          699 c          641 g          267 t
ORIGIN      
        1 gaaccgatca tgtcgccggc ctggtctccc gacggcacgc ggctggccta tgtgagcggc
       61 ggcgacctct ggctgacttg cctcgacggc tcgccaaacc gcctggttca ggcgagtgct
      121 ctccgggcct ccaccggctt ctgggcgcgg aagacacccc tctgcctcga ccccacctgg
      181 acgccggacg gtcaggccat tctgttcctg cactcgacgt tcgacggctt cctgaatctc
      241 taccgagtga acgccgacgg cactggcatc atgctcctcg gcgagacggt ggcgggccca
      301 cccgcgcccc accccactct gccgcggctg gccatcccct atccgggcgg cgtgcggctg
      361 ttggatctct catcgctccc ggagaagcgc tcgatcgtgg cggtgctgcc caaagcggcg
      421 gagccctgct ggtcgcctga tgggtcccgc ctcgtcgtcg tgtgcgacgg cgagctctgg
      481 caaaccaacc ccgacggaga gcaggctcgc aagctgttct ggaacgaccg ctgccccgtg
      541 cgtcagcccg tgtggctccc cgatggccaa accctactcg tgatcgccag cgtgcgacgg
      601 acggccggcc cgcgctacga cgagatctgg ctggtgcctg gccggcccgg cgggggcccg
      661 agggtcctcg ccagcggggg cgaccagggc ggcggccaga gggcaatagg cttcctggcg
      721 tggtgggctc ccggccgtca ggccatctac gggttcgggt accggcccac caccttccgt
      781 tgggtgcgcg aggacgggac ggccggcgac cgggcgtcag gcctcattca gccgtcttgg
      841 cgccccacgg cgtcggtagt cccagcgtcg gccgctgatt ccgaaacacc tcaggaaaag
      901 cagcctggca cctgatgctc agcggcaccg actcaggccc cagatgacgc tgcaggagca
      961 cccagacggc acatctgggt gtgacggagg cgaggccggg agccccaggt tgccgcgaag
     1021 cctcccatcg ggtgttcgcg caggaccagg caacgtgttc agagtgaccc tcgcgagatc
     1081 cgatcgcccg gcccaagggg ccccatgtct gtgccaacgc gcctggtgcg ccggggggcg
     1141 gcacgcggac acccgcgtgg ccaaccatgc cctcgctcct cgccgcggcg cgggtggctc
     1201 agtcggacga gtcgccggga gctcgtgccg tgctctgccc ggcctgctgc tcgagggcgt
     1261 ggatggtcgc cacatcgtcc cctgcagtcc ggctggcgaa gaggaacagc tcgctgcaga
     1321 gcagcatggc ggccggtgcc aacagcaggg cggcctccat ggagcctccc tgggcatcgg
     1381 acactgcgcc cgccgcccaa gcccccgagg cttcacccac caggttgttc gacagggcca
     1441 ggaaccccag cgtcgtcgcc gtcaggtgca ccggcgccaa tgcctgagtg gccaccacgg
     1501 ccagtccgcc ctgccaggcc atgaaaaacg cggtgggggc cagcaggaga aggccggcag
     1561 ccttggacgt gcccagaatc agcaacagga gcaggggcgc gagaaccgct ccgcccgcgg
     1621 ccccacacac ggtcgacacg ttggggcgcc gaggctgagt gcggtcgacg atccacgtgg
     1681 cacaccagaa tccaagtccc ccactcgcca cgctgatgac gccggccgcg acgagctccc
     1741 acaccggtgt ggccccgacg gcgtagcccc tgtgctgctg caggaacgcg ggcccaaaca
     1801 cctgcagcac ggccatcccg aagcagaatg atgtgtcggc gcctaccacg cacagcagac
     1861 tcctccggcg gacgacctcg gagagctcga tccagccctg gcgcgaga
//
LOCUS       DAOSPL010001026         1908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_60432_length_1908_cov_2.954668, whole genome shotgun sequence.
ACCESSION   DAOSPL010001026 DAOSPL010000000
VERSION     DAOSPL010001026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60432_length_1908_cov_2.954668"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PLQ54_16405"
     CDS             complement(<1..424)
                     /locus_tag="PLQ54_16405"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOS94901.1"
                     /translation="MRSVRRGGNVTSMETTHGTRTSAVGRGWLFALAGATLALDQLTK
                     LWVAAAIPLGGARPQTSFFTLTHTTNTGAALGWLRSYPAVLVGVQVLATLVVAYICWR
                     GTLRRRYLAVGLAMVLGGAAGNLVDRLARGHVVDFIKLH"
     gene            complement(394..>1908)
                     /locus_tag="PLQ54_16410"
     CDS             complement(394..>1908)
                     /locus_tag="PLQ54_16410"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOS94902.1"
                     /translation="LRAPALEAVSRVQWVPSEAEQRITGMMATRPDWCISRQRVWGVP
                     FPIFHCPCGAAVFTREATDAVAELFRREGSDAWWQRNPDEILPQGFVCPECGGAASAF
                     RKETDIMDVWFDSGTSYAGVLDVRPDLGFPADVYLEGSDQHRGWFQSSLLTAMAYRGT
                     PPYRTVVTHGFVVDEQGRKMSKSLGNTIDPQEICQTSGADILRWWVCSVDCKRDLHCS
                     AQILEQVANTYVRVRNTCRFLLGNLGDFDPVADGVPYTDLPALERWVLARLAVLIDAA
                     TAAYEAWDFHQVYQDVHSFCAVDLSTVVLDVLKDRLYVEPAASKARRAAQTALYAVLS
                     SLTRLLAPMLTFTTEEIWQVFFRTDDLPTVQLAAWPESQPEWRDDELLARFDRALELR
                     REVYRGIERARESNLFKKPLDGQVAIEAPAEYALLVDQLEAEGLPLAEFLIVSRAVCG
                     TDPVDDPIYESDRIAGLRVAVSRAEGERCPRCWHVVPGLAEGKGLCARCAEVVM"
BASE COUNT          357 a          637 c          632 g          282 t
ORIGIN      
        1 catggagctt gatgaaatcg accacatggc cacgcgccag tcggtccacc aggttgccgg
       61 cggcaccccc caacaccatg gctagcccca cggccagata gcggcgccgg agtgtgcccc
      121 gccagcagat gtaggcaacc accagggtag ccaggacctg gactcccaca agaacagcgg
      181 ggtacgaccg cagccagccc agcgccgctc ctgtgttggt ggtgtgcgtg agagtgaaga
      241 acgacgtctg aggccgggcg ccccccaagg ggatcgccgc cgcgacccac agcttggtca
      301 actggtccag cgcgagcgtg gcccccgcca gcgcaaacag ccaaccgcgc ccgaccgcgc
      361 tggtgcgggt cccatgggtc gtctccatgg acgtcacatt accacctcgg cgcaccgagc
      421 gcatagaccc ttcccttcag cgagccccgg caccacgtgc cagcagcggg ggcaccgctc
      481 tccctcggcc cgggacacgg ccacgcgcag tccggcgatg cggtcgctct cgtaaatggg
      541 gtcgtcgacg ggatccgtgc cgcagaccgc cctggacacg atgaggaact cggccagtgg
      601 aagtccctcg gcctcaagct gatccacgag caacgcgtac tcggcaggcg cctcgatcgc
      661 gacctggccg tcgagcggct tcttgaagag gttggactcc cgggctcgct caatgccgcg
      721 gtagacctcg cggcgaagct cgagcgcccg gtcaaagcga gccaggagct cgtcatcgcg
      781 ccattcgggt tgggactcgg gccacgcggc cagttggacg gtcgggaggt cgtcggtgcg
      841 gaaaaaaacc tgccagatct cctcggtggt gaaggtaagc atgggggcca acaggcgggt
      901 cagcgagctg aggaccgcgt acagagccgt ctgagccgca cgccgggctt tcgacgcggc
      961 cggctcgacg tagaggcggt ctttcagcac atccagcacc accgtgctga gatccacggc
     1021 gcagaagctg tgaacgtcct ggtagacctg atggaagtcc caggcctcgt aggctgcggt
     1081 ggccgcatcg atgagcacgg cgaggcgcgc gaggacccac cgctcgagcg cgggcaagtc
     1141 ggtgtagggc acgccatcgg cgacgggatc gaagtcgccc agattgccca acaggaaccg
     1201 gcaggtgttg cgaacgcgga cgtaggtgtt ggccacttgc tccaggatct gtgccgagca
     1261 atggagatct cgcttgcagt ccaccgagca gacccaccag cggaggatat cggcgccgct
     1321 ggtctggcag atctcctgcg ggtcgatcgt gttcccaagc gacttggaca tcttccggcc
     1381 ctgctcgtcg accacaaacc catgggtaac taccgtgcgg tacggtggtg tgcctcgata
     1441 ggccatggcc gtgagcagtg agctctggaa ccacccccga tgctggtcgc tgccttcgag
     1501 atacacgtcg gcagggaaac caaggtcggg gcgcacgtcg agcacaccag cgtagctggt
     1561 gccggagtcg aaccacacgt ccatgatgtc ggtctctttg cggaacgccg acgcggcgcc
     1621 tccgcactcg gggcagacaa agccctgagg caggatctcg tcaggattgc gctgccacca
     1681 ggcatcggag ccctcacgtc ggaagagctc agccacagcg tcagtggcct cccgggtgaa
     1741 cacggcggca ccgcaggggc agtggaagat cggaaacgga acgccccaca ccctctgccg
     1801 cgagatgcac cagtcgggtc gggttgccat catgcccgtg atccgctgct cggcttcgga
     1861 tggaacccac tggacccggc tcaccgcttc gagcgcgggt gcccgaag
//
LOCUS       DAOSPL010001027         1905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_60556_length_1905_cov_3.108649, whole genome shotgun sequence.
ACCESSION   DAOSPL010001027 DAOSPL010000000
VERSION     DAOSPL010001027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60556_length_1905_cov_3.108649"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(339..1871)
                     /locus_tag="PLQ54_16415"
     CDS             complement(339..1871)
                     /locus_tag="PLQ54_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94903.1"
                     /translation="MRSDLLAALLFAALVVAPARGQADAEWAAATAEELLEAFPECTG
                     VVIPEADSCPAISVGRQASLEDTCDEIARRNTLNWQPVGSAVVFAVPPAEIMDWSAIG
                     ADADQRPRVEPRVRPWALVGRPWSTQAGEWLRSGQGSTWRSVVDYREFSSDTLPNDAW
                     QACQAWLGAMGLISTGPQADGWVLRARAGAVRASWRMGVLDQAIGLCGDVPRIPAPLT
                     LTASGSLQSVLGRVKPPEAAPGLLAADKLHDLPIAVSVRETPADELLCMIAIACGGEW
                     RASPATLVLDDTPRALESLRRVEADLFANGAQEPEHSRHGTRALLIGVSPDDAGHDAI
                     GLGGGTQSLAIEVECGGETRLALLTLWAPPRAIEFALWRDGLPLGIVPGGPHVAVEAL
                     AGFCEGLGRPLPEDVRARIEQLAQTRVPLTRDSLVSLADSAGVELVESGLGLDELGTR
                     NAVAIAHLSWGHAVALTDCDSRFVTLLAEGGLRARFPRRMLESGLSGPLFVDPVVAEG
                     AR"
BASE COUNT          321 a          689 c          641 g          254 t
ORIGIN      
        1 ccctgcgcga gcagcggcga tgtgcagagg gcgaagagga aacacaacgt gcggaacaac
       61 atccgccgtg gaaccggtcc ggctggtttg cctgtgccca tcggatacct ccggtagtag
      121 cacagcacca accccatcag ccgaccacac actagcccgt cagtgcagcc ccgtcaagcc
      181 ctgcaaaaga acacgacgaa tacttctcat gaaaacgctt tcaatagggg ggagcacgaa
      241 ctagcttcct cgccctcgtc ctacgctctc cactgccgct gagaacactg acgccttcgg
      301 ctgcgccggg gcggggctat ggcagccatg agggcccgct acctcgcccc ttcggccaca
      361 accggatcga caaacagagg ccccgacaag cccgactcga gcatccggcg agggaatcgg
      421 gctcgcagac ctccttcggc gaggagggtc acgaaccggc tgtcgcagtc agtcagcgcg
      481 acggcgtggc cccaggagag gtgggcgatg gcgaccgcat tccgcgtgcc gagctcgtcg
      541 aggcccaggc cactctccac cagctcgacg cccgcgctgt cggccaggct gaccaggcta
      601 tctcgagtga gcgggaccct ggtctgcgcc agctgctcga tccgcgccct cacgtcctct
      661 ggcagtggcc ggccaagccc ctcgcagaag ccggccagtg cctcgacggc tacgtgaggc
      721 cccccgggca cgatgcccag cggcaggccg tccctccaca gcgcgaactc gatggcccgc
      781 ggaggggccc agagagtgag cagcgcgagc cgtgtctcgc caccgcactc gacctcgatc
      841 gccagcgact gcgtaccccc accgaggccg atggcgtcgt gcccggcatc gtcgggcgat
      901 actccaatca gcagcgcccg cgtgccgtgg cggctgtgct cgggctcctg agcgccgttg
      961 gcgaacaggt cggcctcgac ccgccgcagc gactcgagag cccgcggcgt gtcgtcgagg
     1021 accagcgtgg ccgggctggc gcgccactcg cccccacacg cgatggcgat catgcacaga
     1081 agctcgtcgg ctggagtctc gcggacggag acggcgatgg ggaggtcgtg aagcttgtcc
     1141 gcggccagca ggccgggggc cgcttcgggc ggcttcacac ggcccagcac gctctgaagg
     1201 cttcccgagg ccgtcagcgt gaggggagcg gggatgcgcg gaacgtcgcc gcagaggccg
     1261 atcgcctggt cgagtacgcc cattcgccag gaggccctca cggcgccggc gcgagcccgc
     1321 agcacccaac catcggcttg cggcccggtg ctgatcagcc ccatggcgcc gagccaggcc
     1381 tggcacgcct gccacgcgtc attgggcaac gtgtctgagg agaactcacg gtagtccacc
     1441 acactccgcc atgttgagcc ctggccgctt cggagccact cgccggcctg ggtcgaccac
     1501 gggcggccga cgagggccca cgggcggacc cggggctcga ccctcggacg ctggtctgca
     1561 tcggcgccga tggcggacca atccatgatc tccgccggcg gaacagcgaa caccaccgcg
     1621 ctgccgacgg gctgccagtt gagggtgttc cgccgggcaa tctcgtcgca ggtgtcctcg
     1681 agggaagcct gccgccccac actgatcgca gggcaggagt cggcctcggg gatgactacc
     1741 cccgtgcatt cggggaaggc ttccagaagc tcctcggccg tggcggcggc ccactcggcg
     1801 tcggcctgtc cgcgagccgg cgcgacgacg agcgcggcga acaacagggc ggccaagaga
     1861 tcacttcgca cttgaggcgc cccccttcag gtcgggcgcg gcggg
//
LOCUS       DAOSPL010001028         1901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_60755_length_1901_cov_2.354280, whole genome shotgun sequence.
ACCESSION   DAOSPL010001028 DAOSPL010000000
VERSION     DAOSPL010001028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60755_length_1901_cov_2.354280"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PLQ54_16420"
     CDS             <1..668
                     /locus_tag="PLQ54_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94904.1"
                     /translation="LQGFVEDHPGYRIESAESWLAEHGQATAALEEAQARAEHQAHRF
                     VELVSFGCILAGVGLGVALYRGGPERADALARKPVLALLHLVLVNKYYLDHAWAAFVR
                     VVVRFEIALAFVDYWVVDGIVRGVGWSTLRVMRWSWAFDKWVVDKVVEAFAWAAGGLG
                     EVLRQTQTGSVHVYMTVTAGVAVAGVLATILGSVWVFAGLFGACVLGAAGWHAALVGR
                     RTV"
     gene            684..>1901
                     /locus_tag="PLQ54_16425"
     CDS             684..>1901
                     /locus_tag="PLQ54_16425"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOS94905.1"
                     /translation="MAEFPILSALVFLPLIGAVVVLFIPRERADAVKSASLATVAASL
                     GLAAMAWASMGLGTAGMQLEEKASWIPQIGISYHLGVDGISMPLVFLTPLVTLLALMY
                     SWIVEDRVKEYFVFFLILQSAMTGVFVALDMFLFYIFWEVSLVPMYFLIGVWGHKPRC
                     EYAAIKFFLYTLAGSVLMLLALIACWWYSGPTLGQRTFDLLELAERQPTLGMSAAGLA
                     TLPMLCFWGFFIGFAIKVPCFPFHTWLPDAHVEAPTAGSVVLAGLLLKLGGYAMIRIL
                     PTLFPTQFKEAAVTVGILALISIIYGSLVAMAQPNLKKLIAYSSIGHMGFVTLGIASG
                     TVEGIQGAVFQMFSHGLLTSALFLLAGILYERAHTYEIDRYGGLGQIVPVYCGIFVFI
                     AMGSLGLPSMSGFV"
BASE COUNT          237 a          567 c          673 g          424 t
ORIGIN      
        1 cgctgcaggg gttcgtagag gaccatcccg gttaccgcat cgagtcggcc gagtcgtggc
       61 tcgccgagca tggccaggcg acagcggcgc tcgaggaggc ccaggcgcgg gctgagcacc
      121 aggcccaccg gtttgtggag ctggtctcct tcggctgcat cctggcggga gtggggctcg
      181 gcgttgccct gtaccgcggc ggtcccgagc gtgcggacgc gctggctcgc aagccggtgt
      241 tggccttgct gcacctcgtt ctggtcaaca agtactacct ggaccatgcc tgggcggcat
      301 tcgtccgggt cgtggtccgg ttcgagatcg ccctggcgtt cgtggactac tgggtcgtcg
      361 atggcatcgt tcggggcgtg ggctggtcga cgctgagggt catgcggtgg tcgtgggcct
      421 tcgacaaatg ggtggtcgac aaggtggtcg aggccttcgc ctgggcggcc ggcgggctgg
      481 gcgaggttct tcgccagacg cagaccggct cggttcatgt ctacatgacc gtcacggccg
      541 gtgtggccgt ggcgggcgtc ttggccacga tcctgggaag tgtctgggtg tttgccggtt
      601 tgttcggcgc ctgtgtgctg ggcgcggccg ggtggcacgc ggcgctggtg ggccggcgaa
      661 cggtgtgagg tgatgagagg gcgatggcgg agttccccat tctgtcagcc ctggtgttcc
      721 tgcccctgat cggggccgtg gtggtcctgt tcatcccgcg ggagcgagcg gatgcggtca
      781 agagcgcgtc tctggccacc gtggccgcgt cgctgggtct ggccgccatg gcgtgggcgt
      841 ccatgggctt gggcacagcg ggcatgcagc tcgaggagaa agcgtcgtgg atcccgcaga
      901 tcgggatctc ctatcacctg ggtgtggacg gcatctccat gcccctggtg tttctcaccc
      961 cgctggtgac gctgctggcg ctgatgtact cgtggatcgt cgaggatcgc gtcaaggagt
     1021 acttcgtctt cttcctgatc ctccagtcgg ccatgaccgg cgtgttcgtc gcgctggaca
     1081 tgttcttgtt ctacatcttc tgggaagtca gcctggttcc catgtacttc ctgatcggcg
     1141 tatggggcca caagccgcgg tgtgagtacg cggccatcaa gttcttcctg tacaccctgg
     1201 ccggcagtgt gctgatgctg ctggcgctga tcgcgtgctg gtggtacagc ggtcccacgc
     1261 tcgggcagcg aaccttcgat ctgctggagc tcgccgagcg gcagcccacg ctcgggatgt
     1321 cggcggcggg tctggccaca ctgcccatgt tgtgcttctg gggcttcttc atcggcttcg
     1381 ccatcaaggt gccgtgcttt ccgttccaca cgtggttgcc cgatgcgcac gtggaggcgc
     1441 cgacggccgg cagtgtggtg cttgcgggtc tcctgctgaa gctgggcggg tacgccatga
     1501 tccgtatcct gccgaccttg ttccccaccc agttcaagga ggctgcggtc actgtcggca
     1561 ttctggctct catcagcatc atctacggct ccctggtcgc catggcccag cccaacctga
     1621 agaagctcat cgcgtactcg agcatcggtc acatggggtt cgtcacgctg gggatcgcgt
     1681 cggggacggt tgagggcatc cagggcgccg tgttccagat gttctcgcac ggcctgctca
     1741 cgtctgccct gttcctgctg gctggcatcc tgtatgagcg ggcgcacacc tatgagatcg
     1801 accgctatgg tggccttggg cagatcgtgc ccgtgtactg tggcattttc gtgttcatcg
     1861 ccatgggttc gctggggctg ccgagcatga gcggtttcgt c
//
LOCUS       DAOSPL010001029         1900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_60786_length_1900_cov_3.393496, whole genome shotgun sequence.
ACCESSION   DAOSPL010001029 DAOSPL010000000
VERSION     DAOSPL010001029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60786_length_1900_cov_3.393496"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLQ54_16430"
     CDS             <1..283
                     /locus_tag="PLQ54_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94906.1"
                     /translation="EAQRDEFIGQFRAFLQAHLCRLDSYLTPAATPAAAAGPMAEQGG
                     DTGAPIDPPPTSDAGDAEDGTGAVLEEDATGNAGEPASPPRWLMAVGDA"
     gene            455..>1900
                     /locus_tag="PLQ54_16435"
     CDS             455..>1900
                     /locus_tag="PLQ54_16435"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOS94907.1"
                     /translation="MASTPAQPIPSPPAAIRHAGSIVPYGVRVALLLAVWAAVGGLEL
                     YDRGRNLWEGGSDADFTQVDVSAQFPAIADALVSSGLPGGASGLGSMPLLYDSLEKQL
                     TRLVEEDTEPSGAAVRVAALLVQRGEPERAVEVIKGLPRGPRLELAPLARGLRDHASP
                     DARRDALGLTDDLRPRWASRALRVYLLEQLGQAAEAQAERQAALRDAMGFIAVAGPAI
                     ALMGLAAVAGVFVLPVYAAVRWNQRTQSRDALRAPSQVAPDATPPDGGADAGFAVPQF
                     APPQSLAAGAQVRAPVARWSVLEAFEAITALFVGQSAMAGLCGLAARAGLALHPVHAL
                     AVQYIGGGLAAVWLAVWRARRAGPDGPRALGIHRFPLWRMLLWGVGGYCGVLPLIVGA
                     GLLLNALSGGSRSINPAIDLARSASHPLDMVAMLLTVAIGAPLVEEILFRGALLGALR
                     TRLGPTLGILLSSTLFCAVHLDPFVAPQLFLL"
BASE COUNT          241 a          687 c          653 g          319 t
ORIGIN      
        1 cgaggctcag cgcgatgagt tcatcggcca gttccgggcg ttcctgcagg cgcacctgtg
       61 ccgcctcgac tcctacctga cgccggcggc aaccccggcc gccgccgccg ggcccatggc
      121 agagcagggc ggtgacaccg gcgcaccgat cgacccgccg cccaccagtg acgccggaga
      181 cgcggaggat ggtaccggtg ccgttctcga agaggatgcc accgggaacg ccggcgagcc
      241 cgcctcgccg cctcgctggc tcatggctgt tggcgacgcc tagacgggct ccctcgggtc
      301 acgtacactc gtatctgcga gccggtgtgg ctcgtacaca ccggtgcgcg cccaatggag
      361 gccgtacgca cccctgccgg ggagacgtga tgtgcctacg ggaaccgctg aacctcccaa
      421 tgaccggcct ctgatggggc cggagcccga cggggtggca tcgactccag cgcagcccat
      481 cccctccccc cctgccgcaa tccgtcatgc cgggtccatt gtgccctatg gtgtccgggt
      541 cgcgctgctg ctggcagtct gggctgccgt gggaggtctg gagctctacg accgtggccg
      601 caacctgtgg gagggaggca gcgatgccga tttcacacag gtggacgtct cggcccagtt
      661 cccggccatc gccgatgccc tagtgagctc agggctcccg ggcggcgcca gtggccttgg
      721 ctccatgccg cttctgtatg acagcctcga gaagcagctg acacggctcg tcgaagaaga
      781 caccgagccg tcgggtgctg cggtaagggt ggctgcactc ctcgtccagc gaggtgagcc
      841 cgagagagcc gtggaggtca tcaaaggcct accccgcggc ccgcgtctcg agctggcacc
      901 ccttgcgcgc ggtctgcgcg accatgcgtc gcctgacgct cggcgcgacg cgctgggtct
      961 gactgacgac ctgcggcccc ggtgggcgag ccgcgccttg cgcgtctacc tgctcgaaca
     1021 gctcggacag gcggccgaag cccaggccga gcgccaggcg gccctgcgcg acgccatggg
     1081 tttcatcgcc gtcgcggggc ccgccatcgc cctcatgggc ttggcggccg tggcgggagt
     1141 gttcgtcctt cccgtctatg cggcggtccg gtggaaccag cgcacccagt cgcgtgatgc
     1201 gctccgggcg ccctcccagg tcgccccaga tgccactccg cccgacggcg gggcggacgc
     1261 ggggttcgcc gtcccccagt tcgcgcctcc tcagtcgctc gcggccggag cgcaggttcg
     1321 agctccggtg gcacgatggt cggtactcga agccttcgag gccatcaccg cactcttcgt
     1381 gggccagagc gccatggcgg gcttgtgcgg cctcgcggcc agagcgggtc tcgccctgca
     1441 tcccgtccac gcgctggctg tccagtacat tggtggcgga ctggccgccg tctggctcgc
     1501 cgtctggcgt gcccggcggg ccgggccaga cggtcctcgc gccctgggaa tccaccgctt
     1561 cccactatgg aggatgctct tgtggggcgt gggggggtac tgcggggtgc tcccgctgat
     1621 cgtgggcgcg ggactgctgc tcaacgcgct gagcggcggc tcgaggtcga tcaaccccgc
     1681 catcgatctg gcccgatcgg cctctcatcc cctcgacatg gtcgccatgc tgctcacggt
     1741 ggccatcggg gcgccgctgg tcgaggagat actgttccgc ggggcactcc tcggggcgct
     1801 gcggacgcgc ctggggccca cgctcggaat cctgctctca tccacgctgt tctgcgcggt
     1861 tcatctcgat ccgtttgtcg ccccccagct gttccttctc
//
LOCUS       DAOSPL010001030         1899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_60822_length_1899_cov_3.505423, whole genome shotgun sequence.
ACCESSION   DAOSPL010001030 DAOSPL010000000
VERSION     DAOSPL010001030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60822_length_1899_cov_3.505423"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1899)
                     /locus_tag="PLQ54_16440"
     CDS             complement(<1..>1899)
                     /locus_tag="PLQ54_16440"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOS94908.1"
                     /translation="ARLADEGETRAAAKLETTVREHIDALASGRTIRGLEYYLPLLYE
                     TRHTLVDYLADDALIVVDEPLRMKAQCEQLLAELGALHETRVRSGELLRLETPVFRDL
                     DQLLDLLAQRQTVHLTLLSPTIPWAPKLPRVDVVSSSAESFRGDLDLLTRSIEDWQAS
                     AQAIVLVTSQRKRLHELLKLRNVAPIREYRPDEHLERRAVHIGHLPVQAGFKIPSAGL
                     VVLADRDIFGWQRAVVPRRRSRDEVQLTSLADLHVGDLVVHINHGIGRYTGIVKKTIE
                     GVERDYLEIQYAGEDRLYVPTPQIDRVQKYVGPEGAVATLHGLNDPRWARTRKKVQQA
                     AEELARDLLRLYATRNQAMGHAFSQDQPWQAEVEDAFIYDETPDQLSAIRDIKQDMEA
                     PKPMDRLLCGDVGYGKTEVAVRAALKCVLDGKQAAVLVPTTVLAQQHLSTFQERLGAY
                     PITVELLSRFRTREQQRRTVDRLASGEVDIVIGTHRLLSRDIAFRDLGLLVIDEEQRF
                     GVRHKESIKQLKEAVDVLTMTATPIPRTLHMSLAGIRDMSLINDPPQGRVAIRTFCVQ
                     EDDNVIREAIERELGREGQVYFVHNRVQSIYHIAQRLKKLVPEARVCVGHGQMHEDDL
                     EQVMIDFYA"
BASE COUNT          306 a          624 c          614 g          355 t
ORIGIN      
        1 gcgtagaagt cgatcatgac ctgctcgaga tcgtcctcgt gcatctggcc gtgccccaca
       61 cagacccggg cctccggcac cagcttcttc agccgctgag cgatgtggta gatcgattgc
      121 acgcggttgt ggacgaagta gacctgtccc tcgcggccca gctcgcgttc aatggcctcc
      181 cgaatcacgt tgtcgtcttc ttgaacacag aatgtgcgga tggcgacccg gccttgcgga
      241 ggatcattga tcaggctcat gtcgcgaata ccggcgagcg acatgtgcag cgtccgcggg
      301 atgggcgtgg ccgtcatcgt gagcacatcg acggcctcct tgagctgctt gatactctcc
      361 ttgtggcgaa cgccgaagcg ctgctcctcg tcaatgacca gcaggcccag gtcgcggaac
      421 gcaatgtccc tggacagcag gcggtgagtg ccgatcacaa tgtccacttc gcccgatgcc
      481 aaccgatcca ccgtgcgccg ctgctgctcg cgggtgcgga agcggctcag cagctcgacg
      541 gtgatcgggt aggctccgag acgctcctgg aacgtgctca ggtgctgctg ggccagtacg
      601 gtggtcggca ccagaaccgc ggcctgtttg ccgtcgagaa cgcacttgag cgcggcccgc
      661 actgccacct cggtcttgcc atagcccacg tcgccgcaca ggaggcggtc catgggcttg
      721 ggagcctcca tgtcctgctt gatatcgcgg atggcgctca gttggtctgg cgtctcatcg
      781 tagatgaacg cgtcctcgac ctcggcctgc catggctggt cttggctgaa cgcatgaccc
      841 atggcctgat tgcgggtggc gtacaggcgc aggagatccc gcgccagttc ctccgctgcc
      901 tgctggacct tcttccgagt ccgcgcccaa cgagggtcat tgaggccgtg caacgtcgcg
      961 acggcgcctt cgggccccac gtacttctgc acgcgatcga tctggggcgt cggaacatag
     1021 agacggtcct cgcccgcata ctggatctcg aggtagtcgc gttccacccc ctcgatggtc
     1081 ttcttcacga tgcccgtata ccggccgatg ccgtggttga tgtggaccac caggtcgcct
     1141 acgtgcaggt cagccaggga cgtgagctgc acttcatccc ggctccggcg gcggggtacg
     1201 accgcgcgct gccacccaaa gatgtcccgg tcagccagta ccacgaggcc cgccgaaggg
     1261 atcttgaaac ccgcctgcac cggaaggtgg ccgatgtgca ccgcgcggcg ctccaggtgc
     1321 tcgtccgggc ggtactcccg gatgggcgcc acgttccgaa gcttcagcag ctcgtgcagg
     1381 cgcttcctct ggctggtgac cagcacgatc gcctgggccg atgcctgcca gtcttcaatc
     1441 gaccgcgtca gcaggtcgag gtcgccacgg aaactctcgg cagatgagct cacgacgtcg
     1501 accctcggga gcttgggcgc ccaggggatg gttggcgaca ggagcgtgag gtgaaccgtc
     1561 tggcgttggg cgagcagatc cagaagctgg tccagatcgc ggaacacggg cgtttccagg
     1621 cgcagcagct cgccgctgcg cacccgggtc tcatgcagag cgcccagctc tgccagtagc
     1681 tgctcgcatt gtgccttcat ccgcaggggc tcgtcgacca cgatcaacgc atcgtcggcc
     1741 aggtagtcaa ccagcgtgtg tcgggtctcg tagaggagtg ggaggtagta ctccaggccc
     1801 cggatagtcc gccctgaggc cagcgcgtcg atgtgctcgc gcacggtggt ctcgagtttg
     1861 gccgcggcgc gcgtctctcc ctcgtccgcc agccgcgcg
//
LOCUS       DAOSPL010001031         1899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_60830_length_1899_cov_2.981562, whole genome shotgun sequence.
ACCESSION   DAOSPL010001031 DAOSPL010000000
VERSION     DAOSPL010001031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60830_length_1899_cov_2.981562"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLQ54_16445"
     CDS             <1..323
                     /locus_tag="PLQ54_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94909.1"
                     /translation="SNSEGWNERTLAPTHGGQTVGSKIYHLETSGPLIGNGAPSMNPW
                     AYYPQLYNSRPHNRDYNYNPSILRYPPPAFPAALNLNTPSAWVERKPVYGTIEGLEFL
                     APQS"
     gene            516..>1899
                     /locus_tag="PLQ54_16450"
     CDS             516..>1899
                     /locus_tag="PLQ54_16450"
                     /inference="COORDINATES: protein motif:HMM:NF039200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 domain-containing protein"
                     /protein_id="HOS94910.1"
                     /translation="MGGLAAVSLLALGSVAAVASDPRLDALNIIWDSPSTDHHGSMPL
                     GNGEIGVNAWVDPSGDLQFYVGRIDSLSAGGQLLKVGKVRIHLDPNPLPDAPSFRQEL
                     DLARGQMLVSFGGANPARLRLWVDANHPVVQVDVDSGRPLQATAKLELWRKTPTEPPP
                     DTRVSDPYSPRVALIQPDVVLHDQPGRIGWYHHNAESFGAVESMAYQGLDGYPGFVDP
                     MLHRTFGGVVAADDGQRIDDETLQAGPGRHVSIRVFSIARHPSTPESWLADMDSTIRE
                     IEAVPEADRYRAHCEWWEAFWDRSFIYINADDETRELRSPIPPNTHPLHVGFDQSGAN
                     VFRGQLGRVAVYNQAYPTERIKQLAACPPERPSPEWSDPVFSGTPAPQTELPDSTDWD
                     QSPGFTVEAWVKTAGPDMVGRIFDKITPGGTDGFLLDTHPGDALRLIVGSDTMHASRP
                     LPAGQWVHVAA"
BASE COUNT          315 a          677 c          622 g          285 t
ORIGIN      
        1 catcgaactc cgaaggatgg aacgagcgca ccctggcgcc gacgcatggc gggcagaccg
       61 tcggctcgaa gatctaccat ctggagacca gcggtccact catcggcaac ggtgcgccga
      121 gcatgaaccc gtgggcctac tacccgcaac tgtacaacag ccggccgcac aaccgggact
      181 acaactacaa tccctccatc ctgcgctacc cacctccggc cttcccggcc gccctcaacc
      241 tgaacacgcc ctcagcatgg gttgagcgca agccggtcta cggcaccatt gagggcctcg
      301 agttcctggc gccgcagagc tgagcatccc gcgggagcgc gagagcggga gacgggcctg
      361 gcgggcctat gcccgccggg cccgcgcgtg tgcggagtcc cctggcagcg cgccctggcg
      421 caggcagccg ccgtgcttcg acgagggcta ggtctggcgg accatcgagc cgaacagaac
      481 ccgtcacttc gacagtccgg agaggacggt gtcccatggg cggtctggcg gccgtgtcgc
      541 tgctggcttt ggggtcggtc gcggccgtag cttcggatcc gcgtctcgat gcgctcaaca
      601 tcatctggga ttcaccgagc accgatcacc acggttccat gccactgggc aacggggaga
      661 tcggggtgaa cgcatgggtc gatccgtctg gcgacctcca gttctacgtt ggccggatcg
      721 atagcctgag tgccggcggc cagcttctca aggtcggaaa ggtgcgtatt cacctggacc
      781 ccaaccccct gccggatgcc ccgagcttcc ggcaggagct ggatctggcg cgcggccaga
      841 tgctcgtctc gttcggcggc gcgaacccgg cgaggctgcg gctgtgggtc gacgccaacc
      901 accccgtggt gcaggtcgat gtcgacagcg gccggccgct gcaggctacc gccaagctcg
      961 agctgtggcg gaagacaccc accgagccac cccccgatac gcgtgtcagc gacccctact
     1021 ccccgcgcgt cgcgctgatc cagcccgatg tcgtgctgca cgaccagccc ggccgcatcg
     1081 gctggtacca ccacaatgcc gagtcctttg gggccgtgga gtccatggcc taccagggcc
     1141 tcgatggcta tcccggcttc gtcgacccca tgttgcaccg caccttcggc ggcgtagtcg
     1201 ccgccgatga cggccagcgc atcgacgatg agacgctgca ggccggcccc ggccggcacg
     1261 tgtcgatccg cgtgttcagc atcgcgcgtc acccctcgac gcccgagagc tggctggccg
     1321 acatggactc gaccatccgc gagatcgagg cggtgcccga ggccgatcgc taccgcgcgc
     1381 actgcgagtg gtgggaggcc ttctgggacc gtagcttcat ctacatcaac gcggacgacg
     1441 agacccggga gttgcggtca ccaatcccgc ccaacacgca cccactgcac gtcggcttcg
     1501 accagagcgg cgcgaacgtc ttccgcggcc agctgggtcg cgtggccgtc tacaaccagg
     1561 cctacccgac cgagcgcatc aagcagctcg ccgcgtgccc gccggagagg ccatcgcccg
     1621 agtggtcgga cccagtgttc tcgggcaccc cggccccaca gacggagctg ccggactcga
     1681 ccgactggga ccagagcccg ggcttcacgg ttgaggcgtg ggtgaagacg gcggggccgg
     1741 atatggtcgg ccgcatcttc gacaagatca cgcccggcgg gaccgacggg ttcctgctcg
     1801 acactcaccc gggtgatgcc ctgcggctca tcgtggggag cgacacaatg cacgcgagtc
     1861 ggccactgcc tgcgggccag tgggtgcacg tggccgcct
//
LOCUS       DAOSPL010001032         1894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_61076_length_1894_cov_3.646003, whole genome shotgun sequence.
ACCESSION   DAOSPL010001032 DAOSPL010000000
VERSION     DAOSPL010001032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61076_length_1894_cov_3.646003"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLQ54_16455"
     CDS             <1..540
                     /locus_tag="PLQ54_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94911.1"
                     /translation="CSAMMCRLAVLVLVAGGCSWGAYECYEIDGSTWVTPGSSATYTC
                     DSDYRPCNPYLHNEVWVSIECAGHPQEGYPGSLWCVADELSLCFYADELEEGDPQTVS
                     ADAELWEDWVDPDDPGEHGLYVGQHTKHQSAITAQIHYRVMPMFMGQGYCVMDTCQSF
                     EGLRRWYSWFGPPDHLACP"
     gene            572..>1894
                     /locus_tag="PLQ54_16460"
     CDS             572..>1894
                     /locus_tag="PLQ54_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94912.1"
                     /translation="MRAILLAAGVVCLAASSDAQVAPDPEAPKTGPYWPEWALRELPA
                     EASAGDIAAALNWVSRQVGVPCIVVSDGEGGGSPAWLGQPATLDGDWQLVAAVGYWAR
                     QHAHIGFVTPRAIVVVPRGNAEPAAGLRESSYGLRSSVVGLAMATAPLDVAAHMAAGA
                     RVLVQQLSEEARACLVRGNVIGRSRQGYPDPLQTPLGLAVSIEPLLRFESDAQGGTHL
                     ARFSLGPGLRQRGEALIRALGFPRAMIFDNQTRGFTVTEWGPDKGGDFPGNPPPELVE
                     SVAEFQADWAAARRRLHALQQIELEFGGPARLGDICRGITACRVDPGPFADVGLWATP
                     GRAPAGSALQCLALAVRASIVAEGDGLRLAEPDGYAIPEPVKVGLRWASAQLPEREDL
                     RLACLAIDDGPLRPFGELDPATRDTVRACIAASEQPPTIVDTTMVRLEP"
BASE COUNT          276 a          635 c          667 g          316 t
ORIGIN      
        1 tgcagtgcga tgatgtgccg gctcgcagtt ctggttctgg tggcgggggg atgcagctgg
       61 ggggcctacg agtgctatga gatcgatgga agcacctggg tcaccccggg gtcctcggcc
      121 acgtacacgt gcgactccga ctaccgcccc tgcaacccct acctgcacaa cgaggtctgg
      181 gtgagcatag agtgtgccgg acacccgcag gagggctacc cgggctccct ctggtgcgtc
      241 gccgacgagc tgtcgctctg cttctacgct gacgaactcg aggagggcga tccccagacg
      301 gtatccgcgg acgccgagct ctgggaggac tgggtcgacc ccgatgatcc aggggaacac
      361 ggactctacg tcgggcagca cacgaagcac cagagcgcga tcacggcgca gatccactac
      421 cgcgtgatgc ccatgttcat gggccaaggc tactgcgtga tggacacctg ccagtccttc
      481 gagggcctcc ggcggtggta ttcctggttc gggcctccgg accatctggc ctgtccgtag
      541 cctggaaccg ggatgcacgg gaggtaacgc gatgcgcgcc atccttctcg ccgctggtgt
      601 ggtctgcctg gccgccagct ccgatgccca ggtagcgccc gacccggagg cgcccaagac
      661 cgggccgtac tggccagaat gggccttgcg cgagctccct gctgaggcgt ctgccggaga
      721 catcgcggcc gccctgaact gggtgtcgcg ccaggtcggg gtgccgtgca tcgtggtatc
      781 ggacggtgag ggtggcgggt cccccgcttg gcttgggcag cccgccaccc ttgacgggga
      841 ctggcaattg gtggccgcag tgggctactg ggctcgccag cacgcgcaca tcggtttcgt
      901 gaccccgcgc gccatcgtgg ttgtgccgcg cggcaatgcc gagccagctg cggggctgcg
      961 cgagagctcc tacggccttc ggtcgagcgt tgttgggctt gcgatggcca ccgcacccct
     1021 cgacgtggcc gcccatatgg ctgcgggcgc ccgggttctt gtgcagcagc tgagcgagga
     1081 ggcccgggca tgcctcgtgc gcggcaatgt gatcggccgc agccgacagg gatacccaga
     1141 cccgttgcag actcccctcg ggctggcagt cagtatcgag cctttactcc ggttcgagtc
     1201 ggacgcgcag gggggcacgc acttggcgcg cttctccctg ggccctggtc tgcgccagcg
     1261 cggcgaagcg ctcatccggg ccctggggtt tccccgggca atgatctttg acaaccagac
     1321 acgagggttc acggtcacgg aatggggacc cgacaagggt ggcgacttcc cagggaaccc
     1381 gccccctgag cttgtcgaga gcgtggcgga gttccaggcg gactgggctg ccgcgcgccg
     1441 gcgactgcac gctctgcagc agatcgagct cgagttcggt gggcccgcaa ggcttggcga
     1501 catctgccga ggcatcacgg cgtgccgggt ggatccgggg cccttcgcgg acgtcgggct
     1561 ctgggccacg ccgggccgtg cccctgccgg ctcggcgctc cagtgcctgg ccctcgccgt
     1621 tcgagcgtcg atcgttgccg aaggcgacgg tctccgactg gcggagcctg atgggtacgc
     1681 catacccgag ccggtaaagg tcggcctccg gtgggcgagc gcgcagttgc cggagcgcga
     1741 ggaccttcgg ctggcctgcc tggccatcga tgacgggccg ctccgcccgt tcggcgaact
     1801 cgacccagcc actcgcgaca ctgtccgcgc gtgcatcgcc gcctccgagc agccgcccac
     1861 cattgtggac accacgatgg tacgactgga acca
//
LOCUS       DAOSPL010001033         1893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_61122_length_1893_cov_3.996736, whole genome shotgun sequence.
ACCESSION   DAOSPL010001033 DAOSPL010000000
VERSION     DAOSPL010001033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61122_length_1893_cov_3.996736"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..973)
                     /locus_tag="PLQ54_16465"
     CDS             complement(143..973)
                     /locus_tag="PLQ54_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94913.1"
                     /translation="MLPETTVGFTPNLMWPAAVKAYTVARSKLKGAGIIEKDRLARNL
                     LSSQPLCFNLFGYLSKVDKNALLPWVRTYAPRATSISRIWLEYAPTAEDLGGDPLGGS
                     AFDAFVEYLLPDESRGFIGIETKYHENLAKGLNIPPEGSRTREKYAKATQLRGAWRVG
                     AAVELVSHRKNLQFWYNQLLAQRTCEFVRDANGAPYTEFTQVVVACRQDESAKAVVRA
                     VADQLAEGHEGTLRFCAIDDVLDSVTGHDDWKREFRQRYTDFTPIQEHLAPGSPLKTG
                     "
     gene            complement(1372..>1893)
                     /locus_tag="PLQ54_16470"
     CDS             complement(1372..>1893)
                     /locus_tag="PLQ54_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017177131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOS94914.1"
                     /translation="LPAIHALPPRRQRAQLDGYLTRIIDRDIPDQGVHVRRPETLRRW
                     MAAYAAASSTSASYTTLLDAASAGEADKPAKTTTIAYRDLLTQIRILDPVPAWLPQGS
                     ELSRLKVAPKHQLADPALAARLLNHTPETLIQPRAGSAELLGQLFESLATLTVRAAAQ
                     AKESSVVPLALLG"
BASE COUNT          300 a          582 c          657 g          354 t
ORIGIN      
        1 ggtcacccgc gcatgcgtgt ccctgctgat ccgtatcgtc gtgctgtcag ccatggacca
       61 acggtacgtc gtgtagacta ttccgtctac acgggttggg gttcgcccgg gccagggccg
      121 cgtatgcggc agagtgccgc ccctaccccg tcttgagggg cgagcctggc gcgaggtgct
      181 cttgaatggg tgtgaagtct gtgtagcgtt gccggaactc gcgcttccag tcgtcgtgac
      241 ccgtgacaga gtcgagaacg tcatcgatgg cacagaaacg aagagttcct tcgtgcccct
      301 cggccagctg gtcggcgacg gcgcgaacca ccgccttggc actctcatct tgccggcagg
      361 cgacgaccac ttgggtgaac tccgtgtagg gcgcgccgtt ggcgtccctg acgaactcgc
      421 aggtccgctg ggcgaggagc tggttgtacc agaactgcag gttctttcga tgcgagacca
      481 gctctaccgc ggcgcccacc cgccaggctc cgcggagctg tgtcgctttc gcgtattttt
      541 cacgggtacg gcttccctct ggggggatgt tcaggccctt ggcgaggttc tcgtggtact
      601 tggtttcgat gccgatgaaa cctcttgact catctggcag caggtactcg acgaaggcgt
      661 cgaaggccga gccgcccagc gggtcgccac ccaggtcctc cgctgtgggg gcgtattcca
      721 accagattcg ggagatggac gtggctcgcg gcgcgtaggt gcgcacccag ggcaggagcg
      781 cgttcttgtc caccttgctg aggtacccga agaggttgaa gcacaggggt tgcgacgaga
      841 gcaggtttcg ggccaaccga tccttctcga tgatcccagc gcccttgagt ttcgatcttg
      901 ccactgtgta ggccttgacg gccgcaggcc acatcaggtt gggcgtgaaa ccaacggtgg
      961 tctccggcag catcgagacc accagcggat tgcggggtcc cgccggcttg ggcggcaggt
     1021 gcagccgccc cgcatcaggg atttcgcggc atacctgacg ccaccaaccc tgctgaagtc
     1081 tcagccccgc ttgcaggggg ttgtcagagc caacccgcca ggccggatcg tgccaaggga
     1141 tattggcgag gctagttgtc atgacgaaaa catatggcac gagttcctgc gggaggcgat
     1201 ttagccctct tcgagcaccg cggcgctgag ttcctgggtg ggagtgagtc cgcagtcgat
     1261 agtcactgtg gacgttggta tgccgccctg cccacgccgg ggtcgaagca atgagttctc
     1321 agccgggccc caccgcgccg caacccgatc gattcgaccc gaccacgtct gtcagccaag
     1381 cagcgcaagg gggacgacgg aggactcctt ggcctgagcg gcggctctga ccgtcaaggt
     1441 ggcgagggac tcgaagagct gcccgagcag ctcggctgat ccggcccgag gttggatcaa
     1501 cgtctccggc gtgtggttga gcagccgcgc ggccagcgcc gggtcagcga gctgatgctt
     1561 gggcgccacc ttgagacggg agagctcact gccctgcggc agccaggccg ggaccgggtc
     1621 gaggatccgg atctgggtca gcaggtcgcg gtacgcaatc gtcgtggtct tggccggctt
     1681 gtcggcctcg ccggcgctgg ccgcatcgag gagggtcgtg tagctcgcgg acgtcgagct
     1741 ggccgcggca tacgccgcca tccaccgccg gagcgtctcg ggccgacgca cgtggacgcc
     1801 ttgatcgggg atgtctcggt cgatgatgcg ggtcaggtag ccgtcgagtt gcgcccgctg
     1861 ccgtctcggt ggcagggcgt ggatggcggg cag
//
LOCUS       DAOSPL010001034         1892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_61191_length_1892_cov_2.473054, whole genome shotgun sequence.
ACCESSION   DAOSPL010001034 DAOSPL010000000
VERSION     DAOSPL010001034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61191_length_1892_cov_2.473054"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1749
                     /locus_tag="PLQ54_16475"
     CDS             <1..1749
                     /locus_tag="PLQ54_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007102744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94915.1"
                     /translation="RESHVQVRQAGHQALLGNYPMIIRWKAHRYLLGQSLGFFQNEFA
                     GRIATWECWNEPNIEFFAGTIAEFADLQKAMAVGVLRGDPEARVLFGGLAGADVPFLE
                     RCYEQGVGPYFDIMAMHPYQWGTTFNDGWFTTKVSSIRDALDRWGDRSKPIWLNELGW
                     STGDAGVTEDVQARLLVQCYVTALGLGHLGAERAFWFCVKDWGGPGYGLYGDDGKKKR
                     AFWSYKHMIIEVRGRECLGRVEAGDARAYLFGPREGQETILLVAWAPGTDAVDVSLPI
                     AGGPFEARDLLGEARQVGSTDGLALTALPDPVYIDVPASAATLAQRPEPREFSASKPD
                     PGTRPLIWASVHPPPGTEHVWLVPGEPREVDLRVFNGTDQAASGEVVLELGGQAFWAH
                     VDVPSMSLDRQWVALSVPEGSPEGMSELRVSGRVGGIDLAAYATPIRVAAGPVVEFLA
                     NSHLERSLYVQPGASCGLSESRRFGSEWVYKLPVARPGRVDLTITVGAHNGRPWSVSW
                     SQDGATWTPLLSGQSWPEPRTAAIAHVDAGDLYLQCKGQDQQVDRVMAVFVGPAASPP
                     SLASRRTRYVHGPVDR"
     gene            complement(1688..>1892)
                     /locus_tag="PLQ54_16480"
     CDS             complement(1688..>1892)
                     /locus_tag="PLQ54_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94916.1"
                     /translation="LTVWAEKDGARTPEYRVELANGRSSFLDAWQLPDGFESVEGRDV
                     FVRFTDPLGRAHTWCGDWRETAD"
BASE COUNT          278 a          637 c          664 g          313 t
ORIGIN      
        1 cgagaaagtc acgttcaggt ccggcaggct gggcaccagg cgctgctcgg caactatccg
       61 atgatcatcc gctggaaggc gcaccgctac ctgctcggcc agagtctagg attcttccag
      121 aacgaattcg ccggccgcat cgccacctgg gagtgctgga acgagcctaa catcgagttc
      181 ttcgccggca ccatcgccga gttcgccgac ctgcagaagg ccatggcagt cggcgtcctc
      241 cgcggcgacc cagaggcgcg cgtcctcttc ggcggcctgg ccggggccga tgtgccattc
      301 ctcgagcgct gctacgagca gggcgtgggg ccctacttcg acatcatggc catgcaccca
      361 taccagtggg ggaccacgtt caacgacgga tggttcacca ccaaggtctc gtccatccgc
      421 gatgcgctcg atcggtgggg cgaccggagc aagcccattt ggctcaacga gctgggctgg
      481 tcgacgggtg atgcgggggt caccgaggac gtccaggccc gtctgctggt ccagtgctat
      541 gtgaccgccc tcgggctcgg gcacctgggt gccgaacgcg cgttctggtt ctgtgtgaag
      601 gactggggcg gtccaggata tgggttgtac ggcgatgatg gtaagaagaa gagagcgttc
      661 tggtcatata agcatatgat catagaggtg cgcggccgcg agtgcctggg gcgggtcgag
      721 gccggcgatg cccgggccta cctgttcggc ccgagggagg ggcaggagac gattctgctc
      781 gtcgcctggg cccctggcac tgacgccgtc gacgtgtccc tgcccatcgc cggcgggccc
      841 ttcgaggccc gggacctgct cggcgaggcc cgccaggtcg gctcaaccga cggcctggcc
      901 ctgacggccc tgcccgaccc ggtctacatc gacgtccccg cttcggcggc gaccctggcc
      961 cagcggccgg agccgcggga gttctcggca tcgaagcccg acccgggcac tcgtccgctg
     1021 atctgggctt ccgtccatcc gcctcccggc accgagcacg tctggctggt gccgggggag
     1081 ccgcgcgagg tcgatctgcg ggtcttcaac gggaccgacc aggcagcctc aggcgaagtc
     1141 gtcctcgagc tgggcgggca ggccttctgg gcgcacgtgg atgtgccctc gatgagcctg
     1201 gaccgtcagt gggtggccct gtcggtcccc gagggcagcc ccgaggggat gagcgaactg
     1261 cgcgtctcgg gccgggtggg cggcatcgac ctggcggcct acgccacacc gatccgcgtg
     1321 gctgcggggc cggtggtcga gttcctcgcg aacagccacc tcgagcggag cctgtacgtg
     1381 cagccgggcg ccagctgcgg gctcagcgag tcgcgccggt tcggcagtga gtgggtctac
     1441 aagcttcccg tggcccggcc cgggcgggtc gacttgacca ttacggtggg cgctcacaac
     1501 ggccggccgt ggagcgtgag ttggtcccaa gatggcgcca cctggacgcc cctgctgtcc
     1561 ggccagagct ggccggagcc gcgcacggcc gccatcgccc atgtcgatgc cggcgacctc
     1621 tacctccagt gcaagggcca ggaccagcag gtcgaccgag tgatggccgt gttcgttggt
     1681 cctgccgcta gtccgccgtc tctcgccagt cgccgcacca ggtatgtgca cggcccagtg
     1741 gatcggtgaa gcgcacgaac acgtcgcgtc cttcgacgct ctcgaagccg tcggggagct
     1801 gccaggcatc caggaacgag ctgcgcccgt tggctagctc gacgcggtac tcgggcgtgc
     1861 gtgccccgtc cttctctgcc cagaccgtca gc
//
LOCUS       DAOSPL010001035         1890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_61262_length_1890_cov_3.359128, whole genome shotgun sequence.
ACCESSION   DAOSPL010001035 DAOSPL010000000
VERSION     DAOSPL010001035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61262_length_1890_cov_3.359128"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PLQ54_16485"
     CDS             complement(<1..479)
                     /locus_tag="PLQ54_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94917.1"
                     /translation="MWSLVSSSAMVALAALSPLAAEDGFRAVFDGVPAWQARPDWLGN
                     ASDHASVSFEDGCVRFSVPEAHRGLKWSLALSSAVWVDTHPYLTVRYRAQGLQASGDY
                     FVYLEDGGPRQLNAVRLDQAVADGEWHTVCVDVTDLAQSDRLTMVAVQVQAGEGPATV
                     "
     gene            complement(586..1347)
                     /locus_tag="PLQ54_16490"
     CDS             complement(586..1347)
                     /locus_tag="PLQ54_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94918.1"
                     /translation="MRRHTRRAIAVVGVVACALTAALCIPSMAQEMAKEKICQLCHQK
                     DQGASPAVTAKYAETLHAKAQGEGPRYNANDGAAGVGCQVCHGPGAEHVKAAAETKKD
                     SIGSPAKLQAREARMSLCARCHAQYEVQGENPADPGFVTDYKYGDDILSKIRLKPMSA
                     EKTKLEQVNEMQNSLHFTSAIGPTCIDCHTGHKDLDPSLPHQTTDAGNAVCLKCHNPE
                     TADVRHCTATFPADALCKSCHMPDNRHIIKVAPAG"
     gene            1632..>1890
                     /locus_tag="PLQ54_16495"
     CDS             1632..>1890
                     /locus_tag="PLQ54_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-structuring protein H-NS"
                     /protein_id="HOS94919.1"
                     /translation="MSEVTERPSMPEKGNWVTWRKEIKVLDCTIRDGGLMNKHRFDDT
                     VVRAVYQACVSAGVDYMELGYRTSKKLASPDEYGPWKFCDEE"
BASE COUNT          325 a          644 c          598 g          323 t
ORIGIN      
        1 acggtggcgg gcccctctcc ggcttgaacc tgaaccgcca ccatggtcag ccggtcactc
       61 tgagccaggt cagtgacatc cacgcacacc gtgtgccact cgccatccgc aacagcttgg
      121 tcgagccgga cggcgttcag ctgtctgggc ccgccatcct cgagatacac gaagtagtcg
      181 cccgaggctt ggagaccttg ggcccggtag cgcaccgtca gatagggatg ggtgtcgacc
      241 catacagccg aggacaacgc gagcgaccac ttgaggcccc ggtgcgcctc ggggactgag
      301 aaccgcacgc acccgtcctc gaaggagacc gacgcgtggt cactcgcgtt gcccagccag
      361 tccgggcgtg cctgccatgc gggcacgcca tcgaaaacgg cgcggaagcc gtcttcggcg
      421 gccagcggcg acagcgccgc cagagccacc atggccgaac tcgagaccag agaccacacg
      481 gcgaccgcct cccgcgaacc aggcaacaag aaggccgccg cgctccatgt gggagcacag
      541 cggcctccag ttcgtcgccc tcagacggcc aacctcgcgg tgagcttacc ccgcgggtgc
      601 caccttgatg atgtggcggt tgtcaggcat gtggcagctc ttgcacagcg cgtcggccgg
      661 gaaggttgcg gtgcaatgcc gcacgtctgc cgtttccggg ttgtggcact tcaggcagac
      721 cgcgttgccc gcatcggtcg tctgatgcgg caagctcgga tcgaggtcct tgtgaccggt
      781 gtggcagtcg atgcacgtcg ggccgatcgc cgacgtgaag tgcaggctgt tctgcatctc
      841 gttgacctgt tcgagcttgg tcttctccgc gctcatgggc ttgagtctga tcttgctgag
      901 aatatcatca ccatacttgt agtcggtgac gaaccccggg tcagccgggt tctcgccctg
      961 gacctcatac tgcgcgtggc accgtgcgca cagggacatg cgggcctcgc gggcttggag
     1021 tttggccggg ctaccgatgc tgtccttctt cgtctcggct gcggccttaa cgtgctcggc
     1081 ccccgggccg tgacacacct ggcaccccac accggcggct ccgtcgttgg cattgtagcg
     1141 aggtccctcg ccctgggcct tcgcatggag cgtctccgca tacttggcgg tgaccgcggg
     1201 agacgctccc tggtccttct gatgacaaag ctggcagatc ttctccttgg ccatctcctg
     1261 tgccatggag gggatgcaca gcgccgcagt gagtgcgcac gccacaaccc ccacgacagc
     1321 gatggctcgc cgcgtgtgtc gtctcatccc gagggtcctc ccctacatgc atcggccgca
     1381 tccgcccgcg gccaaccacc tcatatacga cgcggcgccg cgcacgaggt tcctcgcacg
     1441 cggcacccgg caatcatagc acgggggcct ctgggggagg aagacccctg gcgacgcccg
     1501 gatcggcctt cccccttgcc gcgagaaagg gtaagctctt ggcatgagca ctgtcgcatg
     1561 gcataccacc cgcaccgcgc gtccggtggt gccacgcact ccagcaggcg gaacaggagc
     1621 cggacccgtc catgtcggaa gtcacggaac ggccttcgat gcccgaaaag ggcaactggg
     1681 tgacctggcg caaggagatc aaggtcctcg attgcaccat acgtgacggg ggcctgatga
     1741 acaagcatcg cttcgacgac accgtcgtcc gcgccgtcta ccaggcctgc gtctcggccg
     1801 gcgtcgatta catggagcta ggctaccgga cctcgaagaa gctagccagc cccgacgagt
     1861 acggcccctg gaagttctgc gacgaggagg
//
LOCUS       DAOSPL010001036         1888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_61352_length_1888_cov_3.124932, whole genome shotgun sequence.
ACCESSION   DAOSPL010001036 DAOSPL010000000
VERSION     DAOSPL010001036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61352_length_1888_cov_3.124932"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1768)
                     /locus_tag="PLQ54_16500"
     CDS             complement(113..1768)
                     /locus_tag="PLQ54_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94920.1"
                     /translation="MRYQPETVARLLASLALLAPLASAAGAQPAASVAIDASRPVGPI
                     GELYNVGYDGWGDITNTGMVAAFCDLGVKYCRLVIDLSELCGDAPGDYHWDYARPRDV
                     GLGFTDQVRKVIANGWTPLLAFSYHSGAALLPRWFHGEHHDANQRVWARYNRDGSPAT
                     EGWGDQLGAATGIARDVAAHLAGLGLTGLHWETIYEMGHDMPLVEVHHAVAEGLREAD
                     PTCVIAGPATWPGWTVEERFVKPYLAKYGPDLLDRVSVHWYGSNDHEVRKLWEGEPED
                     WVPTMARRDCLAALLERTKLFGDWTRSLHALLTAPQLNPQAKAIGIIFTEVDVDATSY
                     YMRNPVNDAWPEYRAEADCWLNTNSFGGVWWASVLCHIASAGVAADAAKFTARYYYGL
                     AEMAPDDRAYRYPVWFALRLLREQGGLTSGRQMLQVSCEATAQTEAFATGGPGDLRAI
                     VINKVFTPQSIRLSVSGLAAGNWQATRYVFDETRVARFLGRKPGEEADGLFEGAPTDD
                     SLSARCLEPVDTVPCAEAGGFHTLADFQCPPLSFTVLRFEAAR"
BASE COUNT          288 a          645 c          664 g          291 t
ORIGIN      
        1 ttccattcgg cccctcctct gccagcgcga ggtgttcgga ggggccgaat agggggaacc
       61 atccggagga tggtgggacc ctatccggcc cgaatggctg tctcccccgc cgctaccgcg
      121 ccgcctcgaa ccgcagcacc gtgaagctta gcggcgggca ctgaaagtcg gcgagggtgt
      181 ggaagccgcc cgcctctgcg cacgggaccg tgtcgaccgg ctccaggcaa cgggcactca
      241 gagagtcgtc ggtgggcgcg ccctcgaaca ggccgtcagc ctcctcgcca ggcttccggc
      301 cgaggaagcg cgcgacacgt gtctcgtcga acacataccg cgtggcctgc cagttgcctg
      361 cggccaagcc ggacaccgac agacggatgc tctgtggtgt gaagaccttg ttgatgacga
      421 tcgcccgtag gtcgcccggc ccgccggttg cgaaggcctc tgtctgcgcc gtcgcctcac
      481 acgagacctg tagcatctgg cggccggagg tgagcccgcc ctgctcccgc agcagacgaa
      541 gcgcgaacca gaccgggtag cgataggccc ggtcgtccgg ggccatctcg gccagcccgt
      601 agtagtagcg tgcggtgaac ttggctgcat ccgccgccac gccggccgac gcgatgtggc
      661 acaggaccga cgcccaccac acgccgccga agctgttcgt gttgagccag cagtcggcct
      721 cggcgcggta ctcgggccac gcgtcgttga ccgggttccg catgtagtag ctggtcgcgt
      781 ccacatcgac ctcggtgaag atgatgccga tggccttcgc ctgggggttg agctgcgggg
      841 ccgtgagcag cgcgtgcagc gaccgcgtcc agtcgccgaa aagcttagtt cgctcgagca
      901 gggccgccag gcagtcgcgg cgcgccatgg tgggaaccca gtcctcgggc tcgccctccc
      961 acaacttccg gacctcgtgg tcgtttgagc cgtaccagtg gacgctcacc cggtcgagca
     1021 gatcggggcc gtacttcgcg aggtatggct tcacgaagcg ttcctcgacc gtccagcccg
     1081 gccaggttgc gggtccggca atgacgcagg tggggtcggc ctcgcgcagg ccctcggcca
     1141 cggcatggtg gacctcgacc aggggcatgt cgtgccccat ttcgtagatg gtctcccagt
     1201 gcaggccggt gagcccgagg ccggcgagat gggcagccac atcgcgggcg atgcccgtgg
     1261 ccgcgccgag ctggtcgccc cagccctcgg tcgcgggtga tccatcacgg ttgtaccggg
     1321 cccacacgcg ctggttcgcg tcgtggtgct cgccgtggaa ccaccgtggg agcagcgcgg
     1381 cgccgctgtg gtaggagaag gccagcagcg gcgtccagcc gttggcgatg accttgcgca
     1441 cctggtcggt gaagccgagg cccacatcgc gcggcctggc gtagtcccag tggtagtcgc
     1501 ccggcgcgtc gccacacaac tccgacagat cgatcaccag gcggcagtac tttacgccca
     1561 ggtcgcagaa cgcggcgacc atgcccgtgt tcgtgatatc gccccagccg tcgtagccca
     1621 cgttgtagag ctcgccgatg ggaccgacgg gccgcgaggc atcgatggcg accgaggcag
     1681 ccggctgggc tccggcggcg ctggccagcg gggcgaggag tgccaggctg gcaaggagtc
     1741 gggcgacggt ctctggctgg taccgcatgg ggcccccttg ctcgtccgcg tcggccagca
     1801 tgcccattgt acgggaaccc gaagccaccg gaaagcgagg ccgagggcgc ggcactggcg
     1861 cgggcctacc gcttcctgcg ccatctgg
//
LOCUS       DAOSPL010001037         1887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_61405_length_1887_cov_2.942140, whole genome shotgun sequence.
ACCESSION   DAOSPL010001037 DAOSPL010000000
VERSION     DAOSPL010001037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61405_length_1887_cov_2.942140"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="PLQ54_16505"
     CDS             complement(<1..1014)
                     /locus_tag="PLQ54_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS94921.1"
                     /translation="MKVLSETVWGILHSRLGARLALPARAVSTHRAGFTLVEMLVVLV
                     ILAVLMGLLFIPMFQTVQVLERTNMASVAQDRLGNVMMRLERDISEAQLVIPTPVDWD
                     PDTTDADLPEGHDSTVLSRLDLYLTPAGPAGVVRPSGTFALPFGGGTPTGRVVRYFVA
                     PLPNQPAGPMWGADAAEEVRTGYMNPYIALPGTNLMGPLADPATANMAVLYRVEFDLG
                     EPPFNDPGLYGALYVTNPNDAAYPTSVFGPYFYMNSTPVTWGGSSRPLGTVFRDLAEP
                     LTPTSNADCGLYAWNPSEERHEPQWWGTGTGELPGVLPGLAIVPEAVTDDAAKPDRPS
                     TPEA"
     gene            complement(1109..>1887)
                     /locus_tag="PLQ54_16510"
     CDS             complement(1109..>1887)
                     /locus_tag="PLQ54_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94922.1"
                     /translation="IVALLAGITFASVKNLTSSHEQFKCLSQLRMIHTALGMYQADEL
                     GYPPFDLDEAQRFAEETPTLDAYNVPTWSGGEPAHRTGLWILAATGHLHSVTALHCPS
                     CYEVQAQDTSGNDVDIDDSAGGHNIRSYRDWYDSWYYCSYQNYDREAASWQYMPLRDP
                     TVTSGPGYRRQLWDARLAVPDPVTGDASCPGLQRYSPSGDAILLWCPHHRVDNTSENR
                     RERTLVLFKNGSVEVKDRFVDWVVAGNPAWLFPRNEADWW"
BASE COUNT          323 a          596 c          649 g          319 t
ORIGIN      
        1 cgcctctggg gtcgaaggcc ggtcgggttt cgccgcgtcg tcggtgactg cctcggggac
       61 gatagccagg ccgggcagca caccgggcag ctcgcccgtg ccggtgcccc accactgggg
      121 ctcgtgacgt tcctcgctcg ggttccacgc gtacagcccg caatcggcgt tgctggtggg
      181 ggtcagcggc tcggccaggt cacggaacac cgtgcccaga gggcgactgg atccgcccca
      241 ggttaccggc gtgctattca tgtagaagta gggtccaaat acactggtgg ggtaagccgc
      301 atcattgggg ttggtgacat agagggcccc atagaggccc ggatcgttga agggcggctc
      361 cccgaggtcg aactcgacac ggtagaggac cgccatgttc gcggtggccg gatcggccaa
      421 aggccccatg aggttggttc ccggcagggc gatgtatggg ttcatatagc cggtgcggac
      481 ctcctcggcc gcatcggccc cccacatggg tcccgcaggt tggttgggca gcggggccac
      541 gaagtagcgg accaccctgc ctgtgggggt gccgccgccg aagggcaacg cgaaggttcc
      601 tgacggcctc accacgccgg caggaccggc cggcgtcaga tagagatcga ggcgggagag
      661 cacggtggag tcgtgaccct ctggcaagtc ggcatcagtg gtgtcggggt cccagtcgac
      721 cggagtgggg atgacgagct gggcctcgga gatgtcccgt tcgagccgca tcatgacgtt
      781 gccgagccgg tcctgggcca cggaggccat attggtccgt tcgaggacct ggaccgtctg
      841 gaacatgggg atgaacagca ggcccatcag cacggccagg atgaccagga ccaccagcat
      901 ctcgaccagg gtgaacccgg cccgatgcgt cgagacggcc cgcgccggaa gggccagccg
      961 agcgccaagg cggctgtgaa ggatgcccca cactgtctcg ctcagaacct tcacggttgt
     1021 cgtctcctcc agcccagggc tggccactcg tcccggttca tgattacccg agccgggcct
     1081 cggccaaacg gccagtgcat gcgcgccatt accaccagtc ggcctcgttc cggggaaaca
     1141 gccatgccgg gttccccgcg acaacccagt cgacgaaccg gtctttgacc tcgacactgc
     1201 cattcttgaa caacaccaga gtgcgttcgc gccggttctc gctggtgtta tccacccgat
     1261 ggtgtgggca ccacaggagg atggcgtcgc cactgggcga gtagcgctga aggcccgggc
     1321 acgaggcatc acctgtcacc ggatcgggta cggcaagccg cgcatcccaa agctgcctgc
     1381 ggtatcccgg gccgctcgtg acggtcggat cccgtagagg catgtactgc cacgaggcgg
     1441 cctcgcggtc gtagttctgg tagctgcagt agtaccagct atcgtaccag tcacggtagc
     1501 tccgaatgtt gtgtccaccc gccgagtcgt cgatatcgac atcgttgccg ctggtgtcct
     1561 gcgcctgcac ctcatagcac gaggggcagt ggagggcggt caccgagtgc aggtggcccg
     1621 tagcagcgag gatccacagg cccgtgcggt gcgcgggctc ccccccagac caggtgggca
     1681 cattgtacgc gtccagtgtg ggggtctcct cggcgaaccg ctgggcttcg tcgaggtcga
     1741 agggcgggta tcccagctca tcggcctggt acatcccgag ggccgtgtgg atcatccgca
     1801 actggctcaa gcacttgaac tgctcgtggc tggaggtcag gttcttcacg ctggcaaagg
     1861 tgatgccggc cagcagggcc acgatgg
//
LOCUS       DAOSPL010001038         1885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_61503_length_1885_cov_2.846448, whole genome shotgun sequence.
ACCESSION   DAOSPL010001038 DAOSPL010000000
VERSION     DAOSPL010001038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61503_length_1885_cov_2.846448"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /gene="amrS"
                     /locus_tag="PLQ54_16515"
     CDS             complement(<1..939)
                     /gene="amrS"
                     /locus_tag="PLQ54_16515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOS94923.1"
                     /translation="MNRRDALRLMLSGAVAGCAAGVSACRRPTEAVAEPSSAVVGPEP
                     ELRLAAQGLRSVERWVHPALHYERREKKRVVCRLCPRECLVGDRERGFCGSRENRGGS
                     YYTLVYGQAAAANIDPIEKKPFFHFLPGTRAFSIAAAGCNMDCRACQNWEISQARPEQ
                     IAGARDLPPAQVAAGALQTRCSSIAYTYSEPTVFYEYMLDTAKAGHAQGVRSVMVSGG
                     YIRPGPLRQLLPHLDAVKIDLKSFREETYVDYCRSHLQPVLDAIRVVAGVGKWLEIVY
                     LVVPGVNDSSTEVRDMSRWLLQVAGPGVALHFSRFFP"
     gene            complement(1055..>1885)
                     /locus_tag="PLQ54_16520"
     CDS             complement(1055..>1885)
                     /locus_tag="PLQ54_16520"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HOS94924.1"
                     /translation="YAQQVTYEGLRDTSFAEQIASATAQSSEVLQLLKREGPKYQYGS
                     GCLSDGVIGAWMARIYGIETPLNPRNVKRTLAAIFRHNFVADLADHACPQRPGFALGH
                     EPGLLLCTWPKGDPPTLPFPYSDEVWTGIEYQVASHLIEEGLVKEGLTVTKAVRERYD
                     GRKRNPWNEYECGSYYARAMSSYALLGSLSGFRYSAVDQTLRLAPKLDKDPFRVFIST
                     ATGWGTLTLAGDDLTIDLVEGKLPIRRVVLCRDGHRIERTCRVVARAGVPAVVAALVP
                     "
BASE COUNT          334 a          675 c          571 g          305 t
ORIGIN      
        1 cggaaagaac cgtgagaaat gcagcgccac tcccggtccg gcaacctgga gcagccaccg
       61 gctcatgtct cgcacttcgg tcgagctatc gttcacgccc ggaacgacga gatacacgat
      121 ctcgagccac ttccccacac ctgctaccac tcgaatggcg tcgagcaccg gctgcagatg
      181 gctccgacaa tagtcgacgt aggtctcctc gcggaacgac ttgaggtcga tcttcaccgc
      241 gtccaggtgg gggaggagct gacggagtgg cccgggacgg atgtacccgc ccgagaccat
      301 caccgagcgg acgccttggg catgacccgc cttggccgtg tccagcatgt actcgtagaa
      361 aaccgtcggt tcgctatacg tataggcaat cgaggaacag cgggtctgca acgctcccgc
      421 ggcaacctgg gctggtggaa ggtccctcgc gccggcgatc tgctcgggac gggcttgcga
      481 gatctcccag ttctgacaag cgcggcagtc catgttgcag ccggccgccg cgatcgagaa
      541 cgctcgggtt ccgggcagga agtgaaagaa cggcttcttc tcgatggggt cgatgttcgc
      601 agcagcggct tgcccataga ccagcgtgta gtaggagccg ccgcgattct ccctggaacc
      661 gcagaacccg cgctcccggt cgccaaccag gcactcgcgc gggcacagcc ggcacaccac
      721 gcgcttcttc tctcgtcgct cgtagtggag agccggatgg acccagcgct cgaccgatcg
      781 cagtccctgg gcagccaggc ggagctccgg ttcgggaccc actacagcgg acgaaggctc
      841 cgccacggcc tcagtcggcc gccggcatgc cgaaaccccc gcggcacagc cggccacggc
      901 acccgacagc atgagtcgca acgcatcccg gcggttcacc gtcgaccctc cagcccgacg
      961 tcggcctact tcgccgctcc gcccgaccag cctcccgcgc gccctgcgct ctgtcggcct
     1021 cccaacctcc attccctgcc atcgaccgtc tgccctacgg caccagcgcc gcgaccaccg
     1081 ccggcacccc agctcgagcc acgacccggc acgtcctctc gatccggtgt ccgtctcggc
     1141 acagcaccac acgccgaatg ggcagcttcc cctccaccaa gtcgatggtc aggtcgtcac
     1201 cggcgagggt gagtgttccc cagcccgtag ccgtcgagat gaacacacgg aatgggtcct
     1261 tgtccagctt cggcgccaga cgcagggtct gatccaccgc cgagtaacgg aaaccggaga
     1321 gcgatcccag aagcgcgtag ctcgacatcg cccgcgcata gtagcttccg cactcatact
     1381 cgttccacgg attgcgctta cgaccgtcgt agcgctcccg gaccgctttc gtgaccgtta
     1441 ggccttcctt gaccagccct tcctcgatga ggtgggatgc tacctggtac tcgatcccgg
     1501 tccagacctc gtcggagtag gggaacggca gtgtcggcgg gtcgcccttg ggccaggtgc
     1561 acaggagcag gcccggctca tgccccagcg cgaagccggg gcgctgcgga cacgcgtggt
     1621 cggccagatc ggcaacgaag ttgtgccgga agatggccgc gagcgtcctc ttcacgttcc
     1681 tcgggttcag cggcgtctcg atcccgtaga tccgggccat ccaggcaccg ataacgccgt
     1741 ccgacaagca ccccgagccg tactggtact tgggcccctc gcgcttcagg agctgcagga
     1801 cctcactgct ctgagccgtg gccgaggcga tctgctcggc gaacgacgta tcgcgcagac
     1861 cctcgtacgt cacctgctgg gcgta
//
LOCUS       DAOSPL010001039         1885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_61507_length_1885_cov_2.591257, whole genome shotgun sequence.
ACCESSION   DAOSPL010001039 DAOSPL010000000
VERSION     DAOSPL010001039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61507_length_1885_cov_2.591257"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PLQ54_16525"
     CDS             complement(<1..528)
                     /locus_tag="PLQ54_16525"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOS94925.1"
                     /translation="MPKIKQIALIEPRQSGIRVFSGTALPRLGLPILATELDRMGIKA
                     RVFAEALSPIDWRYVRESQAVFLSSITPTVNRAYALAAEAKSQVPGIATVIGGPHVTF
                     LPEEALGTGAIDYVFRGEADGAMPELISVLSGEAGIADCPGLSGIADGQVAHAPDRPP
                     TRDLDSVPGPDMSLIA"
     gene            complement(653..>1885)
                     /locus_tag="PLQ54_16530"
     CDS             complement(653..>1885)
                     /locus_tag="PLQ54_16530"
                     /inference="COORDINATES: protein motif:HMM:NF025938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOS94926.1"
                     /translation="GVECFRDSPEAPYDWSRQAGQQWFLQAARERGVDRFLAGANSPP
                     VWLTVNGHAYCDPNGPSTNLRPGAEPAFAQFLADVVEHFRAEGYAFRYLSPLNEPNWA
                     WEGGQEGCRFANADIVRLVEALHTELSARRLEVDILVPESGDYPSLLDDERFRAWPGT
                     DDPAAVYMSGNNARGDGKYREYIRDLLGNERLRAMLNNRVSAHSYWTDQGRQHLVDLR
                     RAVRANLDAYAPDATLWQTEYCIMDHTRDLSMDAALHVARVIHHDLVDAEVSAWHWWL
                     AVSPADYKDGLIYTDYRQTGEQNVLCSKTLWVLGNWSRYVRPGFRRVAADLAPARDDV
                     LPSAFVSPDGRRFVIVVVNTSEHDEALQLRVGQDAPSLRRVVTDDTHDLAADQGPTPP
                     GELRIGAGSVVTLLGDVA"
BASE COUNT          308 a          621 c          653 g          303 t
ORIGIN      
        1 agcgatcagc gacatgtccg gtcccgggac gctatcgagg tcgcgtgtcg gcggacggtc
       61 tggtgcgtgc gccacctgcc catcggcaat gcccgagaga cccgggcagt cggcgatccc
      121 ggcctcaccc gagagcacgc tgatcagctc cggcatggcg ccatcggcct cgccgcggaa
      181 gacgtagtcg atggctccgg tcccgagggc ctcctccggc aggaacgtga catgcggtcc
      241 gccgatgacc gtggcgattc ccggcacctg cgacttggcc tcggccgcca gggcgtaggc
      301 ccggttcacc gtgggcgtga tactggacag gaacacggcc tgagactcgc ggacatagcg
      361 ccagtcaatg ggcgacaggg cctcggcgaa cacgcgggcc ttgatgccca tgcgatccaa
      421 ctcggtggcg agtatgggca ggcccaacct cggcaatgcc gtgccgctga agacgcggat
      481 gcccgactgc cgcggctcga taagggcgat ctgtttgatc ttaggcacgc ggaggactcc
      541 ctggcatgcc tgactgggca ggcacgcata gcgatgctga ctcattcgcg gcaccggggc
      601 gggaatcttc caggacctca caaatgacct cgcggccgga cggcgtgatc gcctaagcga
      661 cgtcacccag cagcgtcacg accgagcccg cgccgatgcg cagctcgccg gggggcgtgg
      721 gtccctgatc ggccgccagg tcgtgggtgt cgtcggtgac gacccggcgc agcgatggag
      781 cgtcttggcc cactcggagc tgcagagcct cgtcatgttc cgatgtattc accacaacaa
      841 tgacgaaccg gcggccgtcc gggctgacga acgccgacgg cagcacgtca tcgcgcgccg
      901 gggccaggtc ggccgccacc cggcggaagc ccgggcgcac gtagcggctc cagttcccaa
      961 gcacccacag cgtcttcgag cagagcacgt tctgttcgcc ggtctgccgg tagtcggtgt
     1021 agatgagacc gtctttgtag tcggcggggg acaccgcgag ccaccagtgc cacgccgaga
     1081 cctcggcatc gaccaagtcg tggtggatga cccgagcgac gtggagtgcc gcgtccatcg
     1141 acaagtcgcg cgtgtggtcc atgatgcaat actcggtctg ccatagcgtc gcgtctggtg
     1201 cgtaggcatc gaggttggcg cgaaccgcgc ggcgcaggtc caccaggtgc tgtcggcctt
     1261 ggtcggtcca gtatgaatgg gccgagacgc ggttgttgag catcgcgcgg agccgttcgt
     1321 tgcccagtag gtccctgatg tactcgcggt acttcccgtc gcctcgggcg ttgttgcccg
     1381 acatgtaaac cgcggccgga tcgtctgtcc cgggccaggc gcggaagcgc tcgtcgtcaa
     1441 gcagtgacgg gtagtcgccg ctctcgggca cgaggatgtc cacttcgagt ctgcgggccg
     1501 acagctcggt atggagcgcc tcgacgagac ggacgatgtc ggcgttcgca aaccggcagc
     1561 cttcctggcc gccctcccac gcccagttgg gttcgttgag tggcgacagg taccggaacg
     1621 catagccctc ggcccggaag tgctcgacca cgtcggccag gaactgggcg aaagcaggct
     1681 cggcacccgg tctcaggttg gtcgacgggc cgttcgggtc gcagtaggcg tgcccattga
     1741 cggtcaacca gacaggcggg ctgttggcgc cggcgaggaa ccggtcgacg ccccgctctc
     1801 tggccgcctg gaggaaccac tgctggccgg cctgccgcga ccagtcatac ggcgcctcgg
     1861 gtgagtcgcg gaagcactcg acacc
//
LOCUS       DAOSPL010001040         1885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_61509_length_1885_cov_2.363388, whole genome shotgun sequence.
ACCESSION   DAOSPL010001040 DAOSPL010000000
VERSION     DAOSPL010001040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61509_length_1885_cov_2.363388"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1280
                     /locus_tag="PLQ54_16535"
     CDS             117..1280
                     /locus_tag="PLQ54_16535"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOS94927.1"
                     /translation="MSPRIILVPYLLLLAFFGGVVGLRLGEWYVMQDFVQELRPPMQE
                     FSQYALTLMGSLVGLLTASLSFSKFQRVLGGVEKAPFGDKVAGGVGLLFGAIVGAALV
                     APLREMPVGLKAALLVLSSLLGVSFGLSMKDEIASWFGQGSAGATEKPQQPSANVEAA
                     SDLSAKLLDTNVIIDGRIADICNTGFVEGPLVVPQFVLQELQSIADSDSSLRRARGRR
                     GLVMLARMQKELGDRVQVIEDYDIDLDYRDPVDVRLVRLGKAMGAHILTNDFNLNKVA
                     ELHGVTVLNVNELANALKPVFLPGEDLSVEIVRVGKTAGQGVGYLDDGTMVVVDGGER
                     RIGEEVHCVVTSVLQTVAGKMIFAEIGGDGRPRTEGSNGDKGPRSGPRGRGGR"
     gene            1234..1584
                     /locus_tag="PLQ54_16540"
                     /pseudo
     CDS             1234..1584
                     /locus_tag="PLQ54_16540"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
     gene            complement(1486..1809)
                     /locus_tag="PLQ54_16545"
     CDS             complement(1486..1809)
                     /locus_tag="PLQ54_16545"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HOS94928.1"
                     /translation="MRAGEGWQKRVDGALERCTHFVALLSDEYWVKSNQCLRELYRAV
                     ERFEAGKTEPRLLFVLANEMKPELLKLDGARHGVRGHERSGGVVDRDDLDTSRQRGET
                     QAHRV"
BASE COUNT          298 a          614 c          627 g          346 t
ORIGIN      
        1 atcgaggtgg tgccggtaga gaccattgct cgcgccatgg cactctgtct gcgtgaaggg
       61 aatgggggcg cagtcgcccc gccactagcc gggtcggcaa caggggcggt caatccgtga
      121 gtccacgcat tattcttgtt ccttatcttc ttttgctcgc attctttggc ggcgtcgttg
      181 ggctccgctt gggcgaatgg tacgtcatgc aggacttcgt ccaggaactc cggccgccca
      241 tgcaggagtt cagccagtat gccctgacac tcatgggctc attggtcggt ctcctgaccg
      301 cttctctctc cttcagcaag ttccagcggg tcctgggtgg ggtcgagaaa gcccccttcg
      361 gcgacaaggt cgccggaggt gttggcctgc tctttggcgc catcgtcggg gccgccctcg
      421 ttgctcccct ccgcgagatg ccggtgggac tcaaggccgc cctcctcgtg ctgagcagcc
      481 tcctcggggt cagttttggc ctgagcatga aagacgagat cgcgagctgg ttcggccaag
      541 gatccgctgg cgcgaccgag aaacctcaac agcccagcgc caacgtcgag gcggcgagcg
      601 acctctctgc caaactcctc gacaccaatg tcatcataga cggccgcatc gccgacatct
      661 gtaacacggg gttcgtcgag gggccactgg tcgtccctca gtttgtgttg caggagctcc
      721 agagcatagc ggactcggac agctcgcttc gccgggcccg cggtcggcga gggctcgtca
      781 tgctcgcgcg gatgcaaaag gagctgggcg atcgtgtcca ggtcatcgag gactacgaca
      841 tcgatctcga ctaccgcgac cccgtcgatg ttcgcctcgt gcgtctcggg aaggccatgg
      901 gggcccacat cctgacgaat gactttaacc tcaacaaggt ggctgagctt cacggggtca
      961 cagtgctcaa tgtcaatgag ttggccaacg cgctcaagcc ggtcttcctg cccggcgagg
     1021 acctttccgt cgagatcgtc cgcgtcggca agaccgccgg ccagggcgtc ggatacctcg
     1081 atgacggcac gatggtggtc gtcgacggcg gcgagcggcg gatcggggag gaggtccact
     1141 gtgtcgtgac cagtgtcctc cagaccgtgg ccgggaagat gatcttcgcc gagatcggcg
     1201 gcgacggccg cccgcgcacg gaaggaagca acggtgacaa gggcccacgc tctggtcccc
     1261 gcggccgggg cgggcgatag ggcccgcagg gacgggaaca aggtcctggc cgccatcggc
     1321 ggcatcccca tggtcgcccg cacgctgact gccctggagc actgtagtac catcgaggcg
     1381 atcacggtcg tcgcccgtgc cggcgaggag agccgcatcg cctcgttgtg ccacgagtgg
     1441 ggagtcggca agctgcaccg cgtcgtgacg ggcggcgccg cacggtcaga ctcggtgcgc
     1501 ctgggtctct ccgcgctgcc ggctggtgtc gaggtcgtcg cgatccacga cgccgcccga
     1561 ccgttcgtga cccctgacac cgtggcgcgc gccgtcgagc ttgagcagct cgggtttcat
     1621 ctcgttggcc agcacgaaca gcagccgcgg ctcggtcttg ccggcctcga agcgttcgac
     1681 cgcgcggtag agctcgcgca ggcactggtt cgacttgacc cagtactcgt cgctgagcag
     1741 cgcgacgaag tgtgtgcagc gttcgagcgc gccgtccacc cgcttctgcc agccctcgcc
     1801 ggcgcggatg tcttcgctgt cgagaaaggt gtcgaaaccc gcgccctcgg cgatggcctc
     1861 gagcttcagg tgcagcgcgt tcgcc
//
LOCUS       DAOSPL010001041         1884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_61540_length_1884_cov_3.109896, whole genome shotgun sequence.
ACCESSION   DAOSPL010001041 DAOSPL010000000
VERSION     DAOSPL010001041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61540_length_1884_cov_3.109896"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1548
                     /gene="uvrA"
                     /locus_tag="PLQ54_16550"
     CDS             <1..1548
                     /gene="uvrA"
                     /locus_tag="PLQ54_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOS94929.1"
                     /translation="FAAVFLAAVFFAAVFFAGLSTRLSGARLQIAEPLLKEIGERLQF
                     MVEVGLQYLTLDRSAPSLSGGEAQRIRLATQIGSRLMGCLYILDEPSIGLHARDNRRL
                     LDTLLRLRDLGNTVVVVEHDEETILSADHIVDFGPGAGTQGGHVVASGTVADVLRSEE
                     SVTGQYLSGRRRIPRREERREPRGWLTVRGARENNLRSIDVGFPIGAFTCVTGVSGSG
                     KSTLVCDVLLPALYKHVNRARVRPGAHDSVEGLEYLDRVIHIDQQPIGRTPRSNPATY
                     TGLFGPIRETFAQLPESRLRGYRPGRFSFNVKGGRCEACQGDGLKRIEMLFLPDVYVT
                     CESCKGKRFNRETLQVRYKGASIAEVLDMTVDDALELFRNLPRIARHLETLQQVGLGY
                     IHLGQPATTLSGGEAQRIKLARELGKRATGRTLYILDEPTTGLHFADIEKLLAVLHRL
                     ADAGNTIVVIEHNMEVIRSCDYIVDLGPEGGDDGGLVVCAGTPEQVAAVASSHTGRYL
                     RQALVNN"
     gene            1636..>1884
                     /locus_tag="PLQ54_16555"
     CDS             1636..>1884
                     /locus_tag="PLQ54_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94930.1"
                     /translation="MDRSPGQPGAVALPLRGAGRAMNDSPDSRLVRRCQEGDMEAYCA
                     LASMYRDRVHAFVSRGIRDPSAAEDLVQRFRQARGLGVL"
BASE COUNT          309 a          536 c          676 g          363 t
ORIGIN      
        1 ttcgcggccg tcttcttggc tgcagtcttt tttgcagcgg tcttcttcgc gggcctctcg
       61 acgcggctgt cgggtgctcg tctccagatc gctgagccac tgctgaagga gatcggcgaa
      121 cgtttgcagt tcatggttga ggtgggacta cagtatctga ccctggaccg ttcggctccg
      181 agcctctcgg gaggtgaggc acagaggatt cgcctggcca cccagatcgg gtcacgcctg
      241 atgggttgtc tatacatatt ggatgagccg agtattgggc ttcacgcccg cgacaaccgc
      301 cgtctgctgg acacactcct gcggcttcgc gacctgggca acaccgtggt ggtggttgag
      361 cacgacgagg agacaatcct ctcggctgat cacatcgtgg atttcggccc cggcgcaggg
      421 acgcaaggcg gtcacgtggt ggccagcggt accgtggcgg acgtgttgcg gtcggaggag
      481 tccgtgacgg ggcagtacct ctcggggcgg cgccggatcc ctcgccgcga ggaacgccgc
      541 gagccgcgag gatggctgac ggtccgcgga gcccgggaga acaacctccg gtccatcgat
      601 gtggggttcc ccattggggc gttcacctgc gtgacgggtg tgtcgggtag cggcaagagc
      661 acattggtgt gcgatgtcct gcttccggct ttgtacaagc atgtgaaccg ggcacgagtc
      721 cggcccggcg cgcatgattc ggtggagggt ctcgagtacc tcgatcgcgt cattcacata
      781 gaccagcagc cgatcgggcg gactccgcgc tcgaatccgg cgacctacac gggtttgttt
      841 ggcccgattc gggagacgtt cgcgcagttg ccggagtcga ggctgcgtgg gtaccgtccc
      901 ggccgtttct cgttcaacgt caagggcggc cggtgcgagg cgtgccaggg cgatggtctc
      961 aagcgtatcg agatgttgtt cctgcccgac gtgtacgtga cgtgcgagtc gtgtaagggc
     1021 aaacggttca accgggagac gctgcaggtg cggtacaagg gggcgtcgat cgccgaggtg
     1081 ctcgacatga cggtcgacga tgctctggag ttgttccgga acctgcccag gatcgctcgg
     1141 cacctggaga cgcttcagca ggtgggtttg gggtacatcc acctgggcca gccagcgacg
     1201 actctttctg gtggtgaagc acagcgcatt aagctcgcgc gtgagctcgg caagcgtgcg
     1261 acaggccgca ctctttacat tctggacgaa ccgaccacag ggctccactt cgccgatatc
     1321 gaaaaactac ttgcggtgct gcaccgcctg gccgacgcgg ggaataccat tgtggtgatt
     1381 gagcataaca tggaagtcat ccgatcgtgc gactacattg tcgatctggg gcctgagggc
     1441 ggggatgacg gcggtcttgt ggtgtgtgca gggacgccgg agcaggtggc cgcggtagcg
     1501 agcagccata cgggtcggta cctccggcag gccctggtga acaactgacg gcgccggacg
     1561 tcagtcagga tggagttgag gtgcacgcgg acaacctcgg agatgccgtc cgacatgacg
     1621 cgggaggccg gggagttgga caggagcccg ggacagccag gggcggtggc cctgccgctg
     1681 aggggcgcgg ggcgcgccat gaatgattcg cccgacagcc ggcttgtccg gcggtgccaa
     1741 gagggcgaca tggaggccta ttgcgcgctg gcgtccatgt accgggatcg cgtgcacgcg
     1801 tttgtgagtc gggggatccg cgatccgtcg gccgccgagg atctggtcca gcgtttccgc
     1861 caggctcgcg gccttggcgt gctt
//
LOCUS       DAOSPL010001042         1884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_61545_length_1884_cov_2.897212, whole genome shotgun sequence.
ACCESSION   DAOSPL010001042 DAOSPL010000000
VERSION     DAOSPL010001042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61545_length_1884_cov_2.897212"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1884)
                     /locus_tag="PLQ54_16560"
     CDS             complement(<1..>1884)
                     /locus_tag="PLQ54_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94931.1"
                     /translation="TPFGVFAVVVVCRDQTEIRVYVDGVPEGTRPVSPVEARVHELRL
                     GARRYGGRDQELLRGELAEFILFDRVLSDEERESVERPRLVSEAQRRRGEEYGLEEHR
                     NAHKTRMVGPKILRTWADESAYVRSRSRNEVKDGPPLPLHSLPVRADLLEAIRLSMGC
                     LNNSFDADRDGEPFFYSNCRADGTGEFHHSIEIGIPHVVGRCLLGNMAASLATGIPFP
                     PDGLEILTRYAKSSFDNPDQLNSYTDPRQTGERGVEFHNMREGLYGLWALMHTPEAPW
                     AREQAGRMLTMLDRLTDETGRWSETRVRELLAADRLGGVSVPNAARMVEPLLAVHRLT
                     GDPLALKLAGEYARAGLAEMFLPDGRFAPMDRSSGHVHSITSALSGITEYALMTGHAA
                     MVEACRRILDAGVPEYFSSWGWGDEVYPDHPANEVGRGEINQTGDVIRTALHLGAAGE
                     ARFYELAERYLRSMLLPTQHREEDLRQFLRDKEHPASDAERDVVRRSIGGYSMQLPND
                     RMREGDWPIQTQDITSGAIHAMAECWNHRVTWIADTASVNLLLDYENDRLAVRSELPL
                     AGRIELTARADVTVRLRVPEWVDGRALRVTMADGVRKPKVRGGYVELGVLSPGQTAVV
                     SFE"
BASE COUNT          298 a          654 c          625 g          307 t
ORIGIN      
        1 cctcgaagga gaccactgcc gtctgccccg gcgacagcac tcccagctcg acatagccgc
       61 cgcggacctt gggcttccga acgccgtcgg ccatggtcac gcgcaacgcg cggccatcca
      121 cccactccgg cacccgcaag cggacggtca catcggcgcg tgccgtcagc tcgatccgcc
      181 ccgccagcgg caattccgag cgtaccgcca gccggtcgtt ctcgtaatcg agcagcaggt
      241 tcacgctggc cgtgtcagcg atccacgtga cccggtggtt ccagcattcg gccatggcgt
      301 ggatggcgcc cgatgtgatg tcctgcgtct ggatgggcca atcgccctcg cgcatccggt
      361 cgttcgggag ctgcatcgag tagccaccga tgcttcggcg gaccacgtcg cgttcggcgt
      421 cgctggccgg atgctccttg tcgcgtagga attggcgcag gtcctcctcg cggtgctggg
      481 tcggcagtag catgctgcga aggtagcgtt cagccagttc gtagaaccgc gcctcgccgg
      541 ccgcgcccag atgcagcgcc gtgcggatga cgtcccccgt ctggttgatc tccccacgcc
      601 cgacctcgtt ggccggatgg tcggggtata cctcatcgcc ccagccccac gaactgaagt
      661 actccggaac acccgcgtcg aggatgcgcc tgcaggcttc aaccatggcg gcatggccag
      721 tcatgagcgc gtactcggtg atcccactca gtgccgaggt gatcgagtgc acgtggccgg
      781 aggatctgtc catgggtgcg aagcgcccgt cgggtaggaa catctccgcc aggcccgcgc
      841 gggcgtactc gcccgccagc ttgagcgcaa gcgggtcgcc cgtgagccgg tgaacggcca
      901 gcagcggctc gaccatccgc gcggcgttgg ggaccgacac gcccccgagg cggtcggcgg
      961 ccaggagctc gcggacgcga gtctccgacc accgcccggt ctcatcagtg agtcggtcca
     1021 gcatggtcag catccggccg gcctgctccc tggcccaggg agcctcgggt gtgtgcatga
     1081 gtgcccacag gccgtaaagc ccctcgcgca tgttgtggaa ctcgacgccc cgttcgcccg
     1141 tctgccgcgg atctgtgtat gagttgagct ggtctgggtt gtcgaacgag ctcttcgcgt
     1201 accgggtgag gatctccagc ccatcaggcg ggaacgggat gcccgtggcc agcgaggcgg
     1261 ccatgttccc gagcaggcac cggccgacga cgtgcgggat gccgatctcg atggagtggt
     1321 ggaactcgcc ggtgccgtcg gcacggcagt tcgagtagaa gaagggctcg ccatcgcgat
     1381 cggcatcgaa cgagttattg aggcacccca tggacagccg gatggcctcg agcaggtcgg
     1441 cccgaactgg cagactgtgg aggggcagtg gcggcccgtc cttgacctcg ttgcgcgagc
     1501 gcgaccggac ataggcggac tcgtcggccc aggtgcgaag tatcttgggc ccaaccatac
     1561 gcgtcttgtg ggcgttccga tgctcctcca acccgtactc ctccccgcgc cgccgttgcg
     1621 cttcggagac cagccggggc cgctcgaccg attcacgctc ctcgtccgac aggacccggt
     1681 cgaacaggat gaactcggcc agctcgcccc gcaggagctc ttggtctcgg cctccgtagc
     1741 ggcgggcgcc cagacggagc tcatgcaccc gagcctccac tggcgacaca ggccgagtgc
     1801 cctcggggac gccatcgaca tatacccgga tctcggtctg gtcgcggcag acgacgacca
     1861 cggcgaaaac cccgaacggc gtcg
//
LOCUS       DAOSPL010001043         1881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_61698_length_1881_cov_2.470975, whole genome shotgun sequence.
ACCESSION   DAOSPL010001043 DAOSPL010000000
VERSION     DAOSPL010001043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61698_length_1881_cov_2.470975"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1799
                     /locus_tag="PLQ54_16565"
     CDS             <1..1799
                     /locus_tag="PLQ54_16565"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HOS94932.1"
                     /translation="EGLGALRHCAECGWVDGPTLIQLARNIRDRRFQIMGCAVPEGGP
                     WPWNQDWRVGHEWPLRHFQDYDYYEPRAVPYDIRIPWELSRLWFLVPLLQGEALDPGG
                     EYVEAAEAILADWEDQNPFAASTGWYPMEASMRAISLVLVLDLLAAMKKGAPAFARAL
                     RLAALHGEFIWRTIEYTDVRENHYAANTVALLLLGLALRRVHGPARRWLAYATRHLAA
                     EATTQILPDGVNFEKATGYHRLVTELFLLAHIASGRHGVPWGAEASERLHRACLYSAA
                     CRRPDGLAPCVGDGDDAQVLVFDGRPPRDHSPLIALGAAYFGCGRLKGASSSLSAAVP
                     WLMGPEGVESWRALAADGAFELQYFEAGGVVVVRAGTDWLWIDVGEVGLAGRGGHGHN
                     DLLSFELALDGRPVVVDPGCYCYTADPVARDRFRGSAAHNGLIVDGHEMAELSGLWWI
                     ADDAKPESVSVTRDGEAVCVAAAHAGYARLRDPVRHTRHFRFDTGARLLTLSDRLHCG
                     GSHEVTRFLHLSPGLRLIIEGHRAWVTDDTAGHAVAEVEWGAGTRASACHGEVSPGYG
                     QREPAEVLELADQICGTVTLSLSIRGAGRATP"
BASE COUNT          268 a          625 c          669 g          319 t
ORIGIN      
        1 ctgaaggcct cggcgcgctc cggcactgcg ccgagtgtgg ttgggtggac gggcccacgc
       61 tcatccagct ggcccggaat atccgggatc ggcggttcca gatcatgggg tgcgccgtcc
      121 ccgagggcgg cccgtggccg tggaatcagg actggcgtgt cggccatgag tggccgctcc
      181 ggcacttcca ggactacgac tactacgagc cgcgagcggt gccctacgac atccgcatcc
      241 cctgggagct ttcgcggctg tggttcttgg tcccactgct ccagggcgag gcgctggacc
      301 cgggcggcga atacgtcgag gccgccgagg ccattctggc cgactgggaa gaccagaatc
      361 ccttcgccgc ctccaccggt tggtacccga tggaggcctc catgcgcgcc atatccctgg
      421 tcctggtgct cgacctgctg gcggcgatga agaagggcgc cccggcgttc gcccgcgctc
      481 tgcgactggc cgcgcttcac ggcgagttca tctggcgaac catcgagtat accgatgtgc
      541 gcgagaacca ctatgcggcg aacaccgtgg cactgctcct gctcgggctg gccctgcggc
      601 gagtccatgg cccggcccgc cgatggctgg catatgccac ccggcacctg gccgcggagg
      661 ccacgaccca gatcctgcct gacggggtga acttcgagaa ggccacgggg taccaccgcc
      721 tggtcactga gctgtttctg ctggcacaca tcgcctccgg gcgccacgga gtaccctggg
      781 gtgctgaagc cagtgagcgc ctgcaccgcg cttgcctgta cagcgccgca tgccgccggc
      841 ccgatggtct cgcgccgtgc gtgggcgatg gcgacgacgc ccaggtgctg gtcttcgacg
      901 gccgccctcc tcgcgatcat tcacccctca tcgcgctggg ggccgcatac ttcgggtgcg
      961 ggcggctcaa gggagcctcg agctcactct cggcggcagt cccctggctg atggggcccg
     1021 agggcgtcga gtcatggcgg gcactggctg ccgacggcgc gttcgaactg cagtacttcg
     1081 aagcgggagg agtcgttgtc gtccgcgccg gcaccgactg gctgtggatc gacgtgggcg
     1141 aggtggggct ggctggacgg gggggacacg gacacaacga cctgctgagc ttcgagttgg
     1201 ccctcgatgg ccggcccgtc gtcgtggacc ccggatgcta ctgctacacc gccgacccgg
     1261 tggcacgcga ccgcttccgt ggttccgccg cccacaacgg cctgatcgtc gacgggcacg
     1321 agatggcgga gctctcgggc ctctggtgga ttgccgatga cgcgaagccc gagagcgttt
     1381 cggtcactcg tgacggggag gccgtctgcg tggctgcggc gcacgcgggt tacgctcggc
     1441 tccgcgaccc ggtccgccat acgcgccact ttcgctttga caccggtgct agactactca
     1501 cgctgtccga ccggctccac tgcgggggat cccatgaggt gacgcgtttc ctccatctgt
     1561 cgcccggcct gagactgatc atcgagggcc atcgggcatg ggtgacagac gatacagccg
     1621 gccatgcggt agccgaggtg gagtgggggg ccggcacgcg tgcatcggcc tgccacggcg
     1681 aggtgagccc tgggtacgga cagcgggagc cggccgaggt cctcgagctg gccgatcaga
     1741 tctgtgggac ggtgaccctc tcgttgtcca ttcgcggagc ggggcgagcg acgccatgaa
     1801 gcaggtgttc atgtcggggg ccgggcagat cgacgtgttc gatgtgcctg gggcgctcga
     1861 gatcccgctg cgagccaagc t
//
LOCUS       DAOSPL010001044         1881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_61701_length_1881_cov_2.270537, whole genome shotgun sequence.
ACCESSION   DAOSPL010001044 DAOSPL010000000
VERSION     DAOSPL010001044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61701_length_1881_cov_2.270537"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="PLQ54_16570"
     CDS             <1..959
                     /locus_tag="PLQ54_16570"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HOS94933.1"
                     /translation="LPGPSRRRGVCGSDVHYYEHGRIGPFVVEKPMILGHECAGTVVE
                     VGAHVKGITEGQRVAIEPGVPCRRCRFCHEGKYNLCRDVVFLATPPVDGAFCEYVVSP
                     ADFLFALPDAMTLEQGALVEPLAVGVHAARRGGVKPGDTVAVIGMGTIGLLTLQAAAA
                     HGATRAIAADVQPNRLAAAGQVGATRLVNAASESVVEAVHEETNGLGADVVFETAGTV
                     ATVQQTTECVRPGGVVVLVGLPPESEFPFDVMSLLNREFDVRGVFRYANAYRACVDLI
                     ATGRVNVEPLVTARYRLEEAEEALVFASTRKDSCIKVAVTVP"
     gene            956..1408
                     /gene="dut"
                     /locus_tag="PLQ54_16575"
     CDS             956..1408
                     /gene="dut"
                     /locus_tag="PLQ54_16575"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOS94934.1"
                     /translation="MSERPTIGVRRLPHSHGLPLPAYATAGAAGMDVCAAVEETAFIE
                     SGRVALIPTGLVLEIPQGFEVQVRPRSGLALRHQIAVLNSPGTIDADYRGELQIILAN
                     LGPEPFGVRRGDRIAQLVVARVERALWAERHELSETERGEGGFGHSGR"
     gene            complement(1416..>1881)
                     /locus_tag="PLQ54_16580"
     CDS             complement(1416..>1881)
                     /locus_tag="PLQ54_16580"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HOS94935.1"
                     /translation="RCRTSSARCIVVPTQTRSAEGASHTMAEDRPTGSASVSPWNAVY
                     EEQRPWGGFRQYTHNAISTVKIITVLPGQRLSLQSHERRDELWIALTDGLRVTLGDET
                     RTVAQWEELFVPRGTRHRMEGVGLQSARWLEISFGEFDENDITRYEDDFGRA"
BASE COUNT          284 a          622 c          657 g          318 t
ORIGIN      
        1 gactgccggg tccgagtcgg cgccggggtg tgtgcggctc cgatgtgcac tactatgagc
       61 acggccgcat tggccccttc gtcgtcgaga agcccatgat cctcggccat gagtgcgcgg
      121 gcaccgtggt tgaggtggga gcccacgtga agggcatcac tgagggccag cgggtcgcca
      181 tcgagccggg tgtgccctgc cgccgatgcc gcttctgcca tgagggcaag tacaacctgt
      241 gccgcgacgt ggtgttcctc gcgacgccac cggtcgacgg cgccttctgc gagtacgtcg
      301 tctcgccggc tgatttcctg ttcgccttgc ccgatgccat gacgctcgag cagggcgccc
      361 tggtcgagcc gctggcggtc ggggtgcacg ccgcccggcg cggcggcgtg aaacccggcg
      421 acaccgtggc cgtgatcggc atgggaacca tcgggctcct gaccctgcag gcggccgcgg
      481 cccatggcgc cacgcgcgcc atcgccgccg atgtgcagcc gaaccggctg gcggcagcgg
      541 gccaggtggg ggccacccgc ctggtgaacg ccgcgtccga atccgtggtc gaagccgtgc
      601 acgaggagac caatgggctc ggggccgacg tggtgttcga aacggccggc accgtggcca
      661 cggtgcagca gaccaccgag tgcgtgcgcc cgggcggggt ggttgtgctg gttgggcttc
      721 cgcccgagag tgagttcccg ttcgacgtga tgtcactgct caatcgggag ttcgacgtgc
      781 gaggcgtgtt ccgctacgcc aacgcctatc gggcatgtgt tgatctcatc gccaccggga
      841 gggtgaacgt cgagcccctc gtcacggccc ggtaccgcct cgaagaggcc gaagaggccc
      901 tggtcttcgc aagcacgcgc aaggactcgt gcatcaaggt cgcggtgacc gtgccatgag
      961 tgagaggccg accattggcg tccggcgctt gccccactcg cacgggcttc ctctcccggc
     1021 gtacgccact gcgggggcgg cagggatgga cgtatgcgcc gccgtcgagg agaccgcgtt
     1081 catcgagtcc ggacgggtag cgctaatacc aaccggtctc gtcctcgaga tcccccaggg
     1141 cttcgaggtg caggtccgcc cccgaagtgg cctcgcactg cggcaccaaa tcgcagtgct
     1201 caactcgccg ggcaccatcg acgcggacta ccgtggcgag cttcagatca tcctggccaa
     1261 cctggggccc gagccgttcg gcgttcgccg cggcgaccgc atcgcccagc ttgtggtggc
     1321 acgcgtcgaa cgagccctgt gggccgagcg gcacgagctg tcggagaccg agcgtggcga
     1381 aggcggcttc gggcactcgg ggcgataatg atgcactacg cccggccgaa gtcgtcctca
     1441 taccgggtga tgtcgttctc gtcaaactcc ccgaaggaga tctccagcca gcgggcagat
     1501 tgcagtccga cgccctccat gcggtgccgg gtcccccggg gtacgaacaa ctcctcccac
     1561 tgggccactg tgcgagtctc gtcccccaac gtgacccgga ggccgtccgt cagggcgatc
     1621 caaagctcgt cgcgccgctc gtggctttgg agactgaggc gctggccggg caacacggtg
     1681 atgatcttca ccgtgctgat ggcgttgtga gtgtactggc ggaagccgcc ccacggacgc
     1741 tgctcctcgt agacggcgtt ccagggagag accgatgcgg aacctgtggg tcgatcttcg
     1801 gccatggtgt ggctcgctcc ctcggcggat cgtgtttgcg ttggcacaac gatacacctg
     1861 gctgagctgg ttcgacagcg g
//
LOCUS       DAOSPL010001045         1880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_61735_length_1880_cov_3.153425, whole genome shotgun sequence.
ACCESSION   DAOSPL010001045 DAOSPL010000000
VERSION     DAOSPL010001045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61735_length_1880_cov_3.153425"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1858
                     /locus_tag="PLQ54_16585"
     CDS             <1..1858
                     /locus_tag="PLQ54_16585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS94936.1"
                     /translation="ARGPDPGPKVRSMDSGGRIACSIVVPSHNRGRLLRNTLVSLAHQ
                     SCRRGSFEVIVVDDGSEDGTHQVVKDLNPPYAFQYIAKPYSNSRAQARNWGIRAARGD
                     VLIFLDAEMLCPPGFVSAHLDLHARYGPCMVCGWDRRLAEVPPTPLPEDWEAWARDHL
                     CERQWTPGQGQVGAIPFVTCNASCRRLDAIEVGLFDEAFTGYGHEDLDLGHRLYLLGR
                     RTISSPSTVAYHQPHPRPGWLYVEKDRNSAYFRRKHHEPRRIIHFMDGFWCGGAERSI
                     VSLARHLHGPQHELTLALAEPPVHFGPELAEIGVTVAVADYESAGRLLASRGADAAHV
                     HYAPSGWLDPLRALGDRVPVVASVHLYLTLPAQPGLRRVVYPLYCLAARHPGPREERE
                     VIWLGTDPCAFAPSPCGRLVRRALGLPDDALVFGTGSRLDDVKLTPPMFDVYVELVRR
                     HPKAYVLLCGGGDSLEEYRDRVTSLQLGDRIIMPGTVDDMAAHLGAMDVCLHVVEVET
                     FGCVLLEAMAVGLPVIAPAMDGLAETVRHGTTGFLCSGPDEMLVRALEFANGVHDPKV
                     MGRRARERARTFDDRRTALKFKLLYDEVICGARYSWPWPAALSRSARASASR"
BASE COUNT          272 a          664 c          623 g          321 t
ORIGIN      
        1 ggcgcgcggg ccggacccgg ggcccaaggt gaggagcatg gactcgggag gccggatcgc
       61 gtgtagcatc gttgtcccga gccacaaccg tggccgtctg ctgcggaaca cactcgtgtc
      121 gctcgcccac cagtcgtgtc ggcgcggctc gtttgaggtg atcgtggtcg acgacggctc
      181 ggaggacgga acccaccagg tcgtgaagga cctgaacccg ccatatgcgt tccagtacat
      241 cgccaagccg tactcgaaca gtcgcgccca ggcccgcaac tggggcatcc gggccgcgcg
      301 gggcgatgtt ctgatcttcc tcgacgccga aatgctctgc ccgcccggtt tcgtcagcgc
      361 ccatctcgat ctgcacgccc ggtatggccc ctgcatggta tgtggctggg accgccggct
      421 ggccgaggtg ccaccgacgc ctctacccga ggactgggag gcgtgggccc gggaccatct
      481 gtgcgaaaga cagtggacgc cgggtcaggg acaggtgggc gccatcccgt tcgtcacctg
      541 caacgcgtcg tgccggcggc tcgatgccat cgaggtcggc ctgttcgacg aggccttcac
      601 gggctatggg catgaggacc tggatctggg ccaccggctc tacctgctgg gacgccgcac
      661 catttcgtcc cccagcacgg tggcctacca ccaaccccat cctcggccag gctggctcta
      721 tgtcgagaaa gaccgcaaca gcgcctactt ccggcgcaaa caccacgaac cccggcggat
      781 tatccacttc atggacggct tctggtgtgg tggggccgag cgctccatcg tgtcactggc
      841 gcggcacctg cacggccccc agcatgagct cacgttggcg ctggctgagc ccccagtgca
      901 tttcgggccg gagctcgccg agatcggggt taccgtcgcc gtggctgact acgagtccgc
      961 cggccgcctc ctggcgagcc gtggggccga tgccgcccac gtgcactatg cacccagcgg
     1021 ctggctcgat ccgcttcgcg ctctcggcga ccgcgtaccc gtggtggcga gtgtccacct
     1081 gtatctgact ctcccggccc aaccgggtct gcggcgcgtg gtgtacccgc tctactgctt
     1141 agcggcccgt caccccggcc cgcgggaaga gcgcgaagtc atctggctcg gcacggaccc
     1201 gtgcgccttc gcaccctcgc cttgtgggcg tctggtccgc cgagccctgg gcctgcccga
     1261 cgacgccctg gtgttcggca cgggctcgcg cctcgacgac gtgaagctca cgccgccgat
     1321 gttcgacgtg tacgtcgagc tggtccgccg ccaccccaag gcctacgtcc tgctatgcgg
     1381 cggaggcgat tcgctggagg agtaccgcga ccgggtcacc agcctccagc tcggcgaccg
     1441 catcatcatg ccgggcaccg tcgatgacat ggccgcgcac ctcggggcca tggacgtatg
     1501 cctgcatgtg gtcgaggtcg agaccttcgg ctgcgtcctg ctcgaggcca tggcggtggg
     1561 cctccccgtg atcgctccgg cgatggacgg gctcgccgaa acggttcggc acggcaccac
     1621 tggcttcttg tgttccgggc ccgatgagat gctcgttcgg gccttggagt tcgcgaacgg
     1681 cgtacatgac cccaaggtga tgggacgcag ggcccgcgag cgtgcccgca ccttcgatga
     1741 cagacgcacg gccctcaagt tcaagctgct ctacgacgag gtgatctgcg gagcgcgcta
     1801 ttcctggcct tggccggccg ctctgtcccg ctcggcccgg gcctcagcct ccagataggg
     1861 cacctcgcgc cgcgcgacgg
//
LOCUS       DAOSPL010001046         1880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_61744_length_1880_cov_2.752877, whole genome shotgun sequence.
ACCESSION   DAOSPL010001046 DAOSPL010000000
VERSION     DAOSPL010001046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61744_length_1880_cov_2.752877"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PLQ54_16590"
     CDS             complement(<1..553)
                     /locus_tag="PLQ54_16590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019663977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HOS94937.1"
                     /translation="MSRFRGARTAWRQRRSDLGRLVASGRADVGAHSYGSPTVRTFDH
                     DDTRLVVGRYCSAAHGVTITLGGEHPTDRVTTFPLRTRLNLPGAGTDGFPSSRGDVVI
                     GNDVWIGAHAHLLSGVTIGDGAVVGAGAIVTRDVAPYSIVSGSPARHRRYRVPEDLIP
                     ALLEIAWWNWPDDIVRARVETLSS"
     gene            complement(546..1460)
                     /locus_tag="PLQ54_16595"
     CDS             complement(546..1460)
                     /locus_tag="PLQ54_16595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS94938.1"
                     /translation="MTDFPTMTVGIVLFNGDPGLLDQCLASVAREAAAADVDVEVLLF
                     DNASTEPVRPPAGAVVIRCEDNLGFGRACNRIVSQAGGAVTLLLNPDAALGPGSLTPL
                     LEAARAARPGEMLWGGYLCRDGQGQVDAFWHWWSSAEHLLRWPRVAARLPTARDAPME
                     VEKLCGGALAGFTSDLRTLGPFDESFFLYGEDADLSLRARAAGYRLVLLPAMVVEHYA
                     AASMAAHSGLVEEARADAALRVVALHQPYFYGLLARLDMVLFTLAGLRHGRSSTSARS
                     RLRRLRQVRRWGLHRTVPPFAPRRSPHE"
     gene            complement(1457..>1880)
                     /locus_tag="PLQ54_16600"
     CDS             complement(1457..>1880)
                     /locus_tag="PLQ54_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94939.1"
                     /translation="RSNALAVLERLPNPCGPPCTIAGHGFGSLQEILRQGFGFRYINT
                     VDNQFVSMFWDFGIVGLMAIAVLTLAALRAALRETDPVRAAGAFGVVGLVLGGMFYDV
                     FWNSHGTLLLGLFIAMWVAPPREAASLGPPVARHGSTR"
BASE COUNT          332 a          616 c          670 g          262 t
ORIGIN      
        1 gggaactcag tgtctcgact cgggcccgga caatgtcatc cggccagttc caccaggcga
       61 tctccagcag ggctgggatc aggtcctcgg gcacccggta gcgccgatgc cttgccggcg
      121 aaccggatac gatggagtag ggggcgacgt cccgggtgac gatcgctcca gcgcccacca
      181 cggcgccgtc gccgatggtg accccgctga gcaggtgtgc gtgcgcaccg atccagacgt
      241 cattgccgat cacaacgtct cctcggctgc tcgggaagcc gtcggtgccg gcgcctggaa
      301 ggttcagcct ggtgcgaagg gggaacgtgg tcacccgatc ggtgggatgt tctccgccga
      361 gggtgattgt cacaccgtgc gcagccgagc agtagcgacc gaccactagg cgcgtgtcgt
      421 cgtggtcgaa ggtgcgcacg gttggggacc cgtacgaatg ggcgccgacg tcagctctac
      481 cagaggcgac cagcctgccg aggtcggatc gacgctggcg ccaagccgtc cgcgctcctc
      541 gaaacctact catgtgggct ccttctcggg gcgaacggag gcactgtgcg gtgcaggccc
      601 catcgccgaa cttgtcgtag ccgtcgcaac cggcttcgtg ccgacgtcga ggatcgcccg
      661 tggcgtaagc ctgcgagggt gaacagcacc atgtctagcc gagccagcag gccgtagaag
      721 tacggctggt gcagggccac gacgcgcagg gcggcgtcgg ctcgagcctc ctcgaccagg
      781 ccggagtggg cggccatcga cgctgctgca tagtgctcga cgaccatcgc gggcaggagg
      841 acgaggcggt agccggcagc acgggcgcgc aggctgaggt cggcgtcctc gccgtagagg
      901 aagaacgact cgtcgaaggg gccaagggtg cgcaggtcac tggtgaatcc ggccaaggcc
      961 ccaccgcaca gcttctccac ttccatgggt gcgtcacgag cggtcgggag ccgggcagcc
     1021 acgcgcggcc agcgcagaag gtgctcggcc gagctccacc agtgccagaa ggcgtcgacc
     1081 tggccctggc cgtcgcggca gaggtatccg ccccacagca tctcgcctgg ccgggcggcc
     1141 ctcgcggcct cgaggagggg cgtcaacgag cctggtccga gggcggcgtc cgggttcagc
     1201 aatagggtta cagcgccccc ggcctggcta acgatgcggt tgcaggcgcg accgaacccg
     1261 aggttgtcct cgcaacggat gaccactgcc cctgcggggg ggcggacggg ctcggtgctg
     1321 gcgttgtcga agagcagcac ctcaacgtcc acgtcggccg ctgcggcctc gcgggcgact
     1381 gaggcgaggc actggtcgag cagacccggg tcgccgttga agagcacgat cccgacggtc
     1441 atcgtgggga agtccgtcat cgggtgctcc cgtggcgcgc gaccggcggc ccgagcgagg
     1501 ccgcctccct cggtggcgct acccacatcg cgatgaacag gccgagcagc agggtgccgt
     1561 gcgagttcca gaacacgtcg tagaacatgc ccccgaggac caggccaacg acaccaaagg
     1621 cgccagcagc ccggacgggg tcggtctccc tcagcgcggc acgcagggcc gccaaggtga
     1681 gaacggcaat cgccatcagc cccacgatgc cgaagtccca gaacatcgag acgaactgat
     1741 tgtcgaccgt gttgatgtaa cgaaagccga atccctgccg gagaatctcc tgcagggaac
     1801 cgaagccatg gcctgcaatg gtacacggcg ggccgcaggg gttggggagc cgctcgagca
     1861 cggccagagc gttggaacgc
//
LOCUS       DAOSPL010001047         1877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_61875_length_1877_cov_3.032931, whole genome shotgun sequence.
ACCESSION   DAOSPL010001047 DAOSPL010000000
VERSION     DAOSPL010001047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61875_length_1877_cov_3.032931"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="PLQ54_16605"
     CDS             <1..1492
                     /locus_tag="PLQ54_16605"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94940.1"
                     /translation="LGMDGRAPVVRTWPPSAIELVMQGDFDRFTAVNPKYEDPYPLSE
                     RYFLCSRMTGEGERMGLYLVDVFGNEVLLHVEGQGCFDPMPLSPRERPPVVPDRIDLR
                     GAMGYAYVDDVYRGTGMDAVHRGTVRWLRVVESPEKRFWTQDAWLGQGQEAPAMNWHD
                     FNNKRILGTVPVDEDGSAYFEVPADRFVYFQLLDERGMMVQSMRSGTTFRPGEATGCI
                     GCHEDRGATVPSMRPRTAFHRAPSKLEPWHGTPREFSYITEVQPVFDRYCVSCHDMDQ
                     PAGQKLCLAGDRGLAFNRSYNEIWRKGYVHVVGAGPAEVQEPLTWGSHASRLVQVLLA
                     GHHGVSLDAESFDRLVTWIDLNAPYYPTYATGYPRGAFGRSPLSNDELTRLGALTGIN
                     FLDHVGDQEEEILVSFDRPDMSPVLAAMGDKDAEAYAEALALIEAGAKRLAETDRGDR
                     PTFVLAGIEADRQAKYAERQETAARTRQAMLRGERYYPYRDQAAGR"
BASE COUNT          341 a          587 c          642 g          307 t
ORIGIN      
        1 cctcgggatg gacggccgcg ccccggtggt gcgtacgtgg ccgccgtcgg ccatcgaact
       61 ggtgatgcag ggcgacttcg accggttcac cgccgtgaac cccaagtatg aggacccgta
      121 tccgctcagc gagcgctact tcctctgctc acgcatgacg ggcgagggcg agcggatggg
      181 gctctatctg gtcgacgtgt tcggcaacga ggtgctcctg cacgtcgagg gccaggggtg
      241 cttcgacccg atgccgctct cgccccgaga gcgcccaccc gtggtgcctg accgaatcga
      301 cctccgcggg gccatgggct atgcctacgt cgacgacgtc tatcggggga cgggcatgga
      361 tgccgtccat cggggcacgg tgcgctggct gcgcgtggtg gagtcacccg agaagcggtt
      421 ctggacgcag gacgcatggc tcggccaggg ccaagaggcg ccggccatga actggcatga
      481 cttcaacaac aagcgcatcc tcggaacggt gcctgtcgat gaagatgggt ccgcctactt
      541 cgaggtacct gccgaccgtt tcgtctactt tcagctactc gacgagcgcg ggatgatggt
      601 gcagtcgatg cgtagcggca cgactttccg ccccggcgag gcaacaggtt gcatcggctg
      661 ccacgaggac cgtggtgcca cggtgccatc gatgcgccct cgcacggcct tccatcgggc
      721 accctccaaa ctcgaaccgt ggcacggcac accgcgtgag ttcagctaca tcacggaagt
      781 gcagccggtg ttcgatcgct actgtgtctc atgccacgac atggaccagc cggccgggca
      841 gaagctgtgc ctggcgggcg accgcgggct tgccttcaac cggtcctaca acgagatctg
      901 gcggaagggc tacgttcacg tggtcggcgc cgggccggcg gaagtgcaag agccgctgac
      961 ctggggctcc catgcgagca ggctggtgca ggtgctgctg gcggggcacc acggagtctc
     1021 gctcgacgcc gagagcttcg accgtctggt cacctggatc gatctgaatg cgccctacta
     1081 tccaacctat gccaccggct acccgcgggg agcctttggc cggtcgcctt tgagcaacga
     1141 cgagctgacg cgcctggggg cgctgacggg catcaacttc ctcgaccacg tcggtgacca
     1201 ggaggaggag atcctcgtca gcttcgatcg gcccgacatg agcccggttc tcgcagccat
     1261 gggggacaag gacgcggaag cctatgccga ggcacttgcc ctgatcgagg cgggcgcgaa
     1321 gcggctggcg gagacggacc ggggcgatcg acccacgttc gtcttggccg ggatcgaggc
     1381 cgaccggcag gccaagtatg ccgagcgaca ggagacggcc gcgcggacac gccaggcgat
     1441 gctgcgaggc gagaggtact acccctatcg tgaccaggcg gcggggaggt agggttggaa
     1501 gaagggtcag gagcggatgg ttcggactta gcgcagacgg agcccctagg tagcccggaa
     1561 tgctgagcaa cacgacaggc cagggtgtca tcctgaggag catcagcgac gaaggatctc
     1621 ggccggcagc ggcctccaaa cgccggcaca ggctagcgcc ttcgacgctc ggctggttcg
     1681 agccgagatc cttcgcttcg cagatgctac gcttccaagg acacggccgc gtctggcgag
     1741 cggagagcca cgaaaaccgg tgacagtcac ccatttctag aagttcagaa ataggtgact
     1801 gtcacgaatg gtccgcactt aggcccaatg ccagaggcgt atgtcgcatc acctctgtag
     1861 ccggggcttt agcctcg
//
LOCUS       DAOSPL010001048         1871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_62156_length_1871_cov_3.558370, whole genome shotgun sequence.
ACCESSION   DAOSPL010001048 DAOSPL010000000
VERSION     DAOSPL010001048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62156_length_1871_cov_3.558370"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PLQ54_16610"
     CDS             <1..613
                     /locus_tag="PLQ54_16610"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOS94941.1"
                     /translation="IVERESPDKPIPLAIDEWNVTARDQWNGENRPEVKLREAIYCAR
                     MFHVFHRLGGRITMANIALLVNAMGVLQSDATRVALTPVYRAFQLYGEHTGTHLAHAV
                     VEPSDAPLDVLATVSPETRRTYVAVVNYHPFHDAAAALMLAGGDGARRVRAFRLSGAS
                     PASANALGAEPEVTVTELDAAALAEPAVTFPAHSVTIVEYEAR"
     gene            866..1780
                     /locus_tag="PLQ54_16615"
     CDS             866..1780
                     /locus_tag="PLQ54_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94942.1"
                     /translation="MTLDRLVQAPFNCTMMGSLRGALDYYGIGASDAFLYGASGHAFV
                     LNIHNELCPSGPYCWDRRRVCELLENVGLRLEPIGFFHAGSTPQDRSVAESRLHESLA
                     AGIPCMLTNMEFQLITGTDDTGFLTAQPWACLEFPPSHLTFGTWDELGYGIHMDFHVL
                     HRCEPAEPRKAVLDSLRYAVEVWCRPPGDEGDCYGMGPNGYANWRGAVEAGHGASHGN
                     WWNGVVWAECRARAADYLREVSPLMPIPEAVPGIADGYAAIASALGKCADKELDAPVK
                     LALLAEAEEHEGACVAAIEQQLAAVGSA"
BASE COUNT          294 a          655 c          634 g          288 t
ORIGIN      
        1 catcgtggag cgcgagtcgc ccgacaagcc gatccctctg gccatcgatg agtggaacgt
       61 gactgcccga gaccagtgga acggcgagaa tcggcccgag gtcaagctgc gcgaggccat
      121 ctactgcgcg cggatgtttc acgtgttcca tcggctgggc ggtcggatca ccatggcaaa
      181 catcgccctg ctggtgaacg ccatgggtgt gctccagtcg gacgccaccc gggtcgcgct
      241 cacaccggtc taccgggcgt tccagctcta cggcgaacat acggggaccc acctcgcgca
      301 tgccgtggtg gaaccgagcg acgcccctct cgatgtcctc gccactgtat cacccgagac
      361 ccgccggacc tatgtcgccg tggtgaacta ccacccgttc catgatgcgg cagcagctct
      421 catgctcgcc ggcggcgacg gggcccggcg ggtccgggcc ttccgcctgt cgggcgccag
      481 cccggcttcc gccaacgccc tcggtgctga gcccgaggtc acggtgaccg aactcgatgc
      541 cgcggctctc gccgagccgg cggtcacctt cccggcccac tcggtgacga ttgtggagta
      601 cgaggcgagg tagcgtgaca tctggggccg gctagatcgc caccatccgg ccgacggttg
      661 tcgcgcttcg atccccacgt ggacttccgg tacccggagc agtggcagcg cccacgctcg
      721 cacgaagctc cagaccgcct gccgggaagg ccaagggtgg gtatggggcg aaccgccgcg
      781 ccaacgaccg caggtagctg aggaactgcc gcgagcggct cggcctcggc cggtgcgtct
      841 gccgtcggta cgggaggcac cagccatgac gctcgaccgc ctcgtccagg caccgttcaa
      901 ctgcacgatg atgggctccc tccgaggagc cctcgactac tacggcatcg gtgcatccga
      961 cgccttcctc tacggcgcca gcggccacgc gttcgtcctg aacattcata acgagctgtg
     1021 cccgagcggg ccgtactgct gggaccggcg gcgggtatgc gagctcctcg agaacgtcgg
     1081 gctccgcctg gagccaatcg ggttcttcca tgcgggaagc acgccgcagg atcgttcggt
     1141 tgccgagtcg cggttgcacg agagcctcgc cgccggtatc ccgtgcatgc tcaccaacat
     1201 ggagttccag ctcattacgg gcacggacga caccggcttc ctgacggcgc agccctgggc
     1261 ctgccttgag ttccccccaa gccatctcac gttcggcacc tgggacgagc tcggctatgg
     1321 catccacatg gacttccacg tgctgcaccg gtgcgaacct gcagagccgc gcaaggccgt
     1381 gctggacagt ctgcgctacg ccgtggaagt gtggtgcagg ccccctggag acgagggcga
     1441 ctgctacggc atggggccga acggctacgc gaactggcgg ggggccgtgg aggccgggca
     1501 cggcgcgagc cacggcaatt ggtggaacgg cgtggtgtgg gccgagtgcc gcgcccgagc
     1561 cgcggactac ctgcgcgagg tatcgcccct gatgcccatc cccgaggccg tcccaggtat
     1621 cgcggacggc tacgcggcca tcgcctccgc gctcgggaag tgcgccgaca aggaactgga
     1681 cgccccggtc aagctcgccc tgctcgccga ggccgaggag cacgagggag catgcgtggc
     1741 tgcgatcgag cagcagctcg ccgccgtcgg ctccgcgtag aggccggccc gccatgcaca
     1801 cgtactggcg cagttcggcc gcgccccctt ccccgtgggc cctgaggagc tcatggtggc
     1861 cggcggtggg a
//
LOCUS       DAOSPL010001049         1871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_62158_length_1871_cov_3.237335, whole genome shotgun sequence.
ACCESSION   DAOSPL010001049 DAOSPL010000000
VERSION     DAOSPL010001049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62158_length_1871_cov_3.237335"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1185)
                     /locus_tag="PLQ54_16620"
     CDS             complement(103..1185)
                     /locus_tag="PLQ54_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005551438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS94943.1"
                     /translation="MDRIGVGIIGSQFIADIHAECFRRASGAEVRAVASLNEAHAREF
                     AERHGIGAWHVGHEDLLARDDIDLITVCAPNDVHHRITVDAARAGKHVICEKPLAMTL
                     TQADEMIAACQQAGVKLMYAEELCFAPKYVRAKQLADEGALGRITLVKQSEKHDGPHA
                     PWFWDMKRAGGGVTFDMGCHAIEFFRWVLNKPRVIDVYANMGTYVHGDRTEGDDNALI
                     IVNFENGATGLAEESWTKKGGMDDRAEIHGSLGVTYANLLQGNSLTTYSDVGYGYAVE
                     KAGSTRGWSFTIYEEIWNYGFVQEMQHFVDCVLNDTQPLETGEDGRAVLEVLFAAYES
                     AGTGRKVQLPFTPPEGKRPIELWLGR"
     gene            complement(1273..>1871)
                     /locus_tag="PLQ54_16625"
     CDS             complement(1273..>1871)
                     /locus_tag="PLQ54_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94944.1"
                     /translation="CDAKTAARDQIRLALWRPRDSLEWIEDRMARLADASELPYADGT
                     FDAIARRIDADIKARNDWFSELAVEMLLQSREYGLRPKCSLAIEEVAALLEVHRIDHG
                     RYPGRLDALMPEYAESVPDDPFADGPLHYERTHSGYRLWSPARDRFDAEQTAGYRAAM
                     AAIRAPAPTKPAKPSKPAKPSKPAKPTRRPWPLSGSGH"
BASE COUNT          289 a          652 c          605 g          325 t
ORIGIN      
        1 gtccccctgg cgccgtgcca accatggcgc gcgcccgggt tgcggggcgg ccagcgacat
       61 ccgcgctgac cgcagcccgc gtccgcctcg cctctctttc tcctacctcc ccagccacag
      121 ctcgatcggg cgcttgccct ccggcggagt gaagggcagc tggaccttcc gccccgtccc
      181 cgcactctcg taggcggcga agagcacctc gagcaccgcg cggccgtcct cacccgtctc
      241 gagcggctgg gtgtcgttga gcacgcagtc gacgaagtgc tgcatctcct gaacgaagcc
      301 gtagttccag atctcctcgt agatggtgaa actccaccct cgggtcgagc ccgccttctc
      361 gaccgcgtag ccgtagccca catcactgta cgtagtgagc gagttgccct ggagcaggtt
      421 ggcgtaggtg acgcccaggc tgccgtgaat ctcggcccgg tcatccatgc cgcccttctt
      481 cgtccagctc tcttcggcga ggcccgtggc cccgttctcg aagttgacga tgatcagagc
      541 gttgtcgtcg ccttccgtgc ggtcgccgtg cacatacgtg cccatgttcg catatacatc
      601 gataacccgg ggcttattga ggacccaacg gaagaactcg atggcatggc agcccatgtc
      661 gaaggtgacc ccgccgccgg cgcgcttcat gtcccagaac cacggcgcgt gcgggccgtc
      721 gtgcttctcc gattgcttca ccaacgtgat gcggcccagc gcgccctcat cggccagttg
      781 cttggcccgg acatacttgg gcgcgaagca cagctcctca gcatacatga gcttcacgcc
      841 cgcctgctgg cacgcggcga tcatctcgtc ggcctgcgtc agcgtcatgg ccagcggctt
      901 ctcgcaaatg acatgcttgc ccgcgcgcgc ggcgtcgacg gtgatccgat ggtgcacatc
      961 gttcggcgcg cagacagtga tgaggtcaat gtcgtcccgt gccagcagat cctcgtgccc
     1021 cacatgccag gcaccaatgc cgtgccgctc ggcgaactcg cgtgcgtgcg cctcgttgag
     1081 cgacgccacg gcgcggacct ccgctccgct ggcgcggcgg aaacactcgg cgtggatgtc
     1141 cgcgatgaac tgcgacccga tgatgcccac tccgatgcga tccatggggg ctccctccgg
     1201 aagtgaaatg ccgaccgcga atggcttcgc cagctgaccg gcgagaccta cgcagggccg
     1261 agcggcaggc tctcagtgcc cggagccgga cagtggccac gggcgccggg taggcttcgc
     1321 gggcttcgaa ggcttggcgg gcttcgaagg cttggccggc ttcgtcggcg cgggagcacg
     1381 gatcgcggcc atggccgcgc gatagcccgc cgtctgttcg gcgtcgaacc gatcgcgggc
     1441 cggcgaccac agccggtagc cgctgtgcgt ccgttcgtag tggagcggcc cgtcggcgaa
     1501 tgggtcatcg ggcaccgact cggcatactc cggcatgagg gcgtccagcc ggcctggata
     1561 gcggccatga tcgatccgat ggacttcgag gagagccgcg acctcctcga tggccagtga
     1621 gcacttgggg cgcaggccat actcgcggct ctggagcagc atctcgaccg ccagctccga
     1681 gaaccagtca ttgcgggcct tgatgtcggc gtcgatccgc ctcgcgatcg cgtcgaaggt
     1741 gccatcggca tagggcagtt cggaggcgtc ggccagccgg gccatccggt cctcgatcca
     1801 ctcgagtgaa tcccgggggc gccacagcgc cagacggatc tgatcacgag cggccgtctt
     1861 cgcgtcacag g
//
LOCUS       DAOSPL010001050         1870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_62200_length_1870_cov_2.878788, whole genome shotgun sequence.
ACCESSION   DAOSPL010001050 DAOSPL010000000
VERSION     DAOSPL010001050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62200_length_1870_cov_2.878788"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1870)
                     /locus_tag="PLQ54_16630"
     CDS             complement(<1..>1870)
                     /locus_tag="PLQ54_16630"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS94945.1"
                     /translation="CEPFEAPPNDGYPDVIVVGATPGGIAAAISAARAGSRVLLLTES
                     EHLGGMMSSGLGRTDVGDPNAVGGFAREYLDRVYRFYRDTYGDGSPQVTDCASGTHFE
                     PRVAEALFERMVGETARLTVRRGLRLASVEVLGRRISAIRVIDLRTGWPEWLRADQYV
                     DATYEGDLLALAGVSYVVGREGRDDFGEEHAGHIYWDPERWAVAPGGTGEGDRRVQAY
                     NFRVCLTTDPANRVAIERPVGYDPSRYAVLVRAIRSGRVRRLSDVLSLARLPNGKFDA
                     NNQPAAWLSSDLAEGSERYPEAGPEERAQIVEAHRRHLLGLLYFLQDDASVPASLQAD
                     ARQYGLPRDEFTDARHFPRQLYVREARRMRGRRVFTENDARAAPGQDRPPVQADSIGV
                     GSYALDSHATSAWDASHPNLVEGFFYLGGITRPYEIPYGTLLPREVDNLLVSVCVSAT
                     HVGYSSLRMEPTYMVMGQAAGVAAHLAAALRVGAAEVPIGRLQETLAQGGQVLTASSG
                     VSRATGSDRAVQTPGTRRGFSRYTAEPKALAPTCPDIPTRHPAPAGVDAVAARGMIRT
                     GGPRHGEEPVSAQRVTPPKAAPRGSVAAEWADQAAGVPARSGDRCSPSHAAQRDGP"
BASE COUNT          293 a          675 c          608 g          294 t
ORIGIN      
        1 tccggcccgt ctctctgagc ggcatgcgat ggtgagcacc ggtcgccgct ccgtgccggg
       61 accccagcgg cttggtcggc ccactccgca gccacagagc ctcggggcgc cgccttcggt
      121 ggggtgactc gctgagccga gacgggttcc tcgccgtggc gcggaccgcc cgtacggatc
      181 atgcctcgtg cggccaccgc gtccaccccg gctggggccg ggtgtctcgt gggtatgtct
      241 gggcacgtgg gcgccagagc cttgggctcg gcggtgtatc ggctgaaccc tcgccgcgtg
      301 ccgggcgtct gcacggcgcg gtcactgcct gttgcgcggg acacgccact agaagcagtg
      361 agtacttgcc caccttgggc cagggtctcc tgcagacggc cgataggtac ctcggctgcc
      421 cccacacgga gagccgccgc caggtgcgcc gccacccccg ccgcctggcc catcaccatg
      481 tatgttggct ccatgcggag tgagctatag cccacatggg tggccgagac acatacactc
      541 acgagtagat tgtccacctc gcggggcaag agcgtgccgt acggaatctc gtatggccgt
      601 gtgatacccc cgaggtagaa gaagccctcg accaggttcg gatgcgacgc gtcccaggcc
      661 gacgtggcgt gagagtctag tgcatagctc cccacgccta tgctgtcggc ctgaaccggt
      721 ggccgatcct ggccgggcgc ggcccgggcg tcgttttcgg tgaaaacgcg gcgcccacgc
      781 attcgccggg cctcacggac atacagctgc cgcgggaagt ggcgggcatc cgtgaactcg
      841 tctcggggca gtccatactg gcgcgcatcc gcctgaagtg atgcgggcac cgatgcgtcg
      901 tcctgaagga agtagagcag cccaagcagg tggcgacggt gcgcctccac gatctgggct
      961 ctctcctcgg gtcctgcctc tggataccgc tcactgccct ctgccaggtc cgatgacagc
     1021 cacgcagcgg gttggttgtt cgcatcgaac ttcccgttgg ggagccgcgc cagtgagagc
     1081 acgtccgaca gccgccggac acgccccgac cgaatcgccc gcaccaaaac cgcgtaccgc
     1141 gatggatcgt agcccacggg ccgctcgatc gcgacgcggt tggccggatc ggtggtcagg
     1201 catacccgaa agttgtaggc ctgtacccgc cggtcgccct cgccggtccc accgggtgcc
     1261 acggcccacc gctcgggatc ccagtagatg tgacctgcgt gctcctcccc gaagtcgtcc
     1321 cggccctcgc ggcccaccac ataagagacc ccggccaacg ccagcaggtc cccctcgtag
     1381 gtcgcatcga catactggtc cgcgcgcagc cattcgggcc agccggtgcg caggtcgatc
     1441 acccggatgg cgctgatgcg gcggccgagg acctccaccg aggccagcct cagccccctg
     1501 cgcacggtga gccgtgcggt ctcccccacc atccgctcga acagcgcttc ggccactcgg
     1561 ggctcaaagt gggttcccga cgcgcaatca gtcacctgcg gtgagccgtc accatacgtg
     1621 tcgcgataga agcggtaaac gcggtcgaga tactcccggg cgaacccccc caccgcattc
     1681 gggtcgccca cgtccgtacg gcccagaccg gacgacatca tcccgcccag atgctcgctc
     1741 tcggtgagga gaagcacgcg tgaaccggcc cgggccgccg agatggccgc tgcgattccc
     1801 ccgggcgttg ccccgaccac gatcacatcc gggtagccat cgttcggggg tgcctcgaag
     1861 ggctcgcagg
//
LOCUS       DAOSPL010001051         1869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_62245_length_1869_cov_3.096472, whole genome shotgun sequence.
ACCESSION   DAOSPL010001051 DAOSPL010000000
VERSION     DAOSPL010001051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62245_length_1869_cov_3.096472"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..>1869
                     /locus_tag="PLQ54_16635"
     CDS             173..>1869
                     /locus_tag="PLQ54_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94946.1"
                     /translation="MRVRTGWSRLAPLAAIGVALIGAVFTNRMAVGQAPDGGALRSGV
                     CPPFALRDEQGRVINPVSGENADQPYSPKQTCGACHDYDKITQGYHFTQGAGESPTEA
                     QAERVQWATTPGLYGGSWCSPAPLYCYLSPKANASARTMDLTSFTLMAGGCGDCHPGG
                     GPAEYDREGKRYDTWMADPASGLTSGGDNGLDGDYYKARWADSGVLEADCLICHQPEY
                     DLAERNRQKALLNLRWAATAGAGLAKVAGSVKDGTPVEVSYDKARFGPDGKLLLGAQG
                     KTKLHLVREPRDEACLGCHAQPGWKKRGADYRPRTDVHLRAGLKCVDCHPAGSSASDP
                     RIYGYEQHQIGKGDDPGGLVRDDLDNTVRSCAECHTTRRLGAPIPKHEGLPETHLQTL
                     SCQVCHIPERSVAAIMVQAGDVLNPGPHIPVKNKPTWTFYGPDLSYLNHYANLGVQGY
                     DDKPTSPYRPVLARYKGVIYPVNRVHSAWPAIETDGLPGLMEPTPQDIQAMWVAHQQD
                     ASVYPRLASITDDNGDLVPEVNRPDEIDALIASVTEMLNATHYPMEGKRVLWVMNDRA
                     "
BASE COUNT          349 a          667 c          591 g          262 t
ORIGIN      
        1 taccagtggg acctgcgcca gacgacatgg gcagcaacct acctgctgca cggacgagag
       61 gatcgtgtcc agctagccta cgtcacggcg gccgacggcc agggcaggcg cgctccggac
      121 aaggccctgc tacaattcca gaggttcttc tgatcgagag tcgaggtgct gcatgagagt
      181 acgcacagga tggtcccgcc ttgcgccgct cgccgccatc ggcgtggccc tgattggcgc
      241 ggtgttcacg aaccggatgg cggtcggcca agctcccgac ggcggcgcgc tgcgatcggg
      301 tgtctgtcca cccttcgcgc tccgcgatga gcaaggccgt gtcatcaacc cggtgagtgg
      361 ggagaacgcc gaccagccct actcgccgaa gcagacctgt ggcgcctgcc atgactacga
      421 caagatcacg cagggctacc acttcaccca gggcgcgggc gagagcccca ctgaggccca
      481 ggcggagagg gtccagtggg cgactacccc cgggctctat ggcggctcat ggtgctcgcc
      541 agccccccta tactgctacc tctcgcccaa agcaaacgct tcggcgcgca ccatggacct
      601 cacgtctttc accctcatgg ccggcggctg cggcgactgc cacccaggtg gtggaccggc
      661 cgagtacgac cgcgagggca agcggtacga tacatggatg gccgacccgg ccagcggcct
      721 aacgagtggg ggcgacaacg gcctcgacgg tgactactac aaggcgcggt gggccgactc
      781 cggcgtcctc gaggccgact gcctcatctg ccaccagccc gagtacgacc tcgccgagcg
      841 caacaggcag aaggcactcc tgaaccttcg ctgggccgca accgcgggcg ccggcctcgc
      901 gaaggttgcg ggctcagtca aggacggcac gcccgtcgag gtcagctacg acaaggcccg
      961 gttcggcccc gacgggaagc tcctgctcgg cgcgcagggc aagacgaagc tccacctcgt
     1021 ccgcgagccg cgggacgaag catgtctcgg ctgccacgca caacctgggt ggaagaagcg
     1081 tggggccgac tatcgcccca ggaccgatgt ccacctgcgc gccggcctca agtgcgtcga
     1141 ctgccacccg gccggatcga gtgcgtctga cccgcggatc tacggctatg aacagcatca
     1201 gatcggcaag ggcgacgacc ctgggggact ggtccgcgat gacctggaca acacggtgcg
     1261 ctcctgcgcc gagtgccaca ccacccgccg cctgggggct ccgatcccca agcacgaggg
     1321 actcccagag acgcatctgc agaccctctc gtgtcaggtg tgccacatcc cggagcgatc
     1381 tgtcgccgcg atcatggtcc aggccggcga cgtactcaac cccgggccgc acatcccggt
     1441 gaagaacaag ccgacgtgga ccttctacgg ccccgatctg agttacctga accactacgc
     1501 gaacctgggc gtgcaaggct acgacgacaa accgacctcg ccataccgcc cggttctcgc
     1561 gcgctacaag ggcgtcatct accccgtgaa tcgcgtccac agcgcctggc cggccatcga
     1621 aaccgacggc ctgcccgggc tcatggagcc gacgccgcag gacatccagg ccatgtgggt
     1681 ggcccaccag caggacgcct ccgtctaccc tcggctggcc tcgatcaccg acgataacgg
     1741 cgacctcgtg cccgaggtga accggcccga cgagatcgac gcgctcatcg cctcggtcac
     1801 cgagatgctc aacgccaccc actaccccat ggagggcaaa cgcgtcctgt gggtgatgaa
     1861 cgatcgggc
//
LOCUS       DAOSPL010001052         1869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_62253_length_1869_cov_2.733738, whole genome shotgun sequence.
ACCESSION   DAOSPL010001052 DAOSPL010000000
VERSION     DAOSPL010001052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62253_length_1869_cov_2.733738"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="PLQ54_16640"
     CDS             <1..1167
                     /locus_tag="PLQ54_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOS94947.1"
                     /translation="SEDGGSIPALALGTAEGFDDILDCAAAQPAGPIDIDIDEADDAL
                     IIYTSGTTGKPKGALFDQHRVLWVGINSALGLSLREGERMLHVAPLYHSASLNMLIIG
                     GMMYGATHLILPAFDPERVLETIESERATFFFGVPTMYTLMLRSPSMTTRDLSSLRVC
                     LYGAAPMPATTAESIVAALPAATIIQACGQTEGGPGGILLQHDEVLAQPTASGRYALP
                     NTEVRVVDPDGNDAEPGEVGEMIMRGETMMKRYWRKPEATADTIRNGWVHTGDLASID
                     ADGYITIVDRMKDIIITGGRNVYSAEVESALAGHPAVGDIAVVGRPDDVYGERVVAII
                     TPRNRETPTLEELCAYGLKHLSSFKLPRELIIDEIPRNASGKIVKHRIREKLDA"
     gene            1160..>1869
                     /locus_tag="PLQ54_16645"
     CDS             1160..>1869
                     /locus_tag="PLQ54_16645"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOS94948.1"
                     /translation="MPDVELTVDGGVATLELARPERRNALSRAAGHELIHALDRVAQD
                     DSARVLILTGTGGSFCAGADLTDLDPDRLGDAMAGFNRIIPALRGMPQPVIAKVRGSA
                     VGAGCSLALACDFVLADDTARFCQIFPRLGLSTDLGGSFILPRLVGMRRARELALLGD
                     DVDGRTAATIGLIMRCVPADELDAAVKDLAMRLAGFSPSAQARTKHLLDETWNGTLTD
                     ALAREAEAQIANASTPEF"
BASE COUNT          343 a          657 c          580 g          289 t
ORIGIN      
        1 tccgaggacg gaggcagcat tccagctctc gctctcggaa ccgccgaagg gttcgacgac
       61 atcctcgact gcgcggccgc ccaacctgcc gggccgatcg acatcgacat cgacgaggcc
      121 gacgatgccc tcattatcta tacgtcgggg accacgggaa agccaaaggg cgccctcttc
      181 gatcaacacc gcgtcctctg ggtcggcatc aactccgcgc ttggcctcag cctgcgcgag
      241 ggcgagcgga tgctccatgt ggcgccgctc taccactccg ccagcctcaa tatgctcatc
      301 atcggcggga tgatgtacgg cgccacccac ctgatccttc ccgccttcga cccggagcgg
      361 gtcctcgaga cgatcgagag cgagcgggcg accttcttct tcggggtgcc gacgatgtac
      421 accctgatgc ttcggagccc ttcgatgacc acgagggacc tctcgagcct ccgcgtctgc
      481 ctctacggcg ccgcgccgat gccggcgacg actgccgagt cgatcgtcgc cgcgctgccc
      541 gcagcgacca tcatccaggc ctgcggccag accgaaggcg gcccgggcgg catcctcctg
      601 cagcacgatg aggtgctcgc ccagcccacc gccagcggtc ggtacgccct tccgaacacg
      661 gaggtgcgcg tcgtcgaccc cgacgggaac gatgcggagc ccggggaggt cggcgaaatg
      721 atcatgcgcg gggaaactat gatgaagcga tactggcgga aaccggaggc caccgcggat
      781 accatcagga acggttgggt acacacaggt gaccttgcca gcatcgacgc ggacggttac
      841 atcaccatcg tggaccggat gaaggacata atcatcaccg gcggccggaa cgtttactca
      901 gccgaagtgg agagtgccct ggccggtcac ccggcggtgg gagatatcgc cgtggtcgga
      961 cgcccggatg atgtgtacgg cgaaagggtc gtggccatca tcaccccgcg caacagggag
     1021 actcccacgc tcgaggaact ctgcgcctac ggactgaagc atctgagctc cttcaagctt
     1081 ccacgcgagc tcatcattga cgaaatcccg cgcaacgcct ccggcaaaat cgtcaagcac
     1141 cgcatccggg agaagctcga tgcctgatgt ggaactcact gtggacggcg gcgtcgccac
     1201 gcttgaactg gcgcggcccg agcgccgcaa cgcactcagc cgggcggcag gtcacgagct
     1261 gatccacgcc ctggaccgtg tagcccagga cgacagcgcg cgcgtcctga tcctcaccgg
     1321 cacagggggc agcttctgcg ccggcgcgga cctgacagac ctcgaccccg accgactcgg
     1381 cgacgccatg gcagggttca acaggatcat ccccgccctg cgcgggatgc cgcagccggt
     1441 catcgccaag gtccgtggaa gcgccgtggg tgccggctgc agcctcgcac tcgcctgcga
     1501 cttcgtcctc gcggacgaca cggcccgctt ctgccagatc ttcccccggc tcggactctc
     1561 aactgacctt ggcggtagct tcatcctgcc caggctcgtc ggaatgcgcc gtgcccggga
     1621 gcttgcgctc ctcggcgacg acgttgacgg ccgtaccgcc gcaacgatcg ggctcatcat
     1681 gcgctgtgtg cccgctgacg agctggatgc ggcggtcaag gacctggcca tgcgactggc
     1741 cggcttctcg ccctcagccc aggcacgcac caaacacctc ttggacgaga cctggaacgg
     1801 aaccctcacg gacgcactgg cccgtgaggc tgaggcccag attgcgaatg cctcgacacc
     1861 cgagttcaa
//
LOCUS       DAOSPL010001053         1868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_62294_length_1868_cov_3.226145, whole genome shotgun sequence.
ACCESSION   DAOSPL010001053 DAOSPL010000000
VERSION     DAOSPL010001053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62294_length_1868_cov_3.226145"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1868
                     /locus_tag="PLQ54_16650"
     CDS             <1..>1868
                     /locus_tag="PLQ54_16650"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOS94949.1"
                     /translation="ISHPFYVGACEVTNAQYERFDPDHRRLRGRLGFSNGDDEAVVFV
                     SWHEAAAFCHWLSEREGLPYRLPSEAEWEYACRAGTTTPYWCGDTLAPVFHKNQRMTW
                     YPSTPLRTDAATGLPTLDVVDLSVGRTPPNPWGLFDMHGNVEEWCRDWYGPYAAGAQT
                     DPAGPAGGEFRVTRGGSHSTEPYFLRSSNRMGTLPDDRHWLIGFRVALGEAPRAVAPP
                     APKPRHLRRVDPAPARWRPVSADAPVFRGPTPYVRPPAGGAPWMLGHNHDPALTVCPN
                     GDLLAVWYSCAEETGRELKQLASRLRRGQTEWEPASVFFAPPDRNAHCPALWCDRQGV
                     LHHFAGLSIASTWGALAGVMRTSEDSGATWSPARLIMPEHGARHMPVAATELSDGTLA
                     LTCDAVTGGTGATALHLSADGGRTWTDAGGNILGIHASVVELADGSLMALGRGDTVDG
                     RMARSLSRDRGRTWSYSASPFPPLSSGQRLALIRLREGPLFLASFAKGMPLVDSAGET
                     RRVSGLFAALSFGDGATWPCTRLVAESGETRLGETTDGQPCALGHFDAEPIGYLSACQ
                     APDGLIHLISSRNHYAMNLAWLRQGQPPLPPALRPGALEARTALSHAVSPPHLPAA"
BASE COUNT          246 a          689 c          660 g          273 t
ORIGIN      
        1 ccatctcgca tccgttctat gtgggggcct gcgaggtcac caacgcccag tacgagcgct
       61 tcgacccgga ccaccggagg ctgcgcggcc gcctcgggtt ctccaatggc gacgatgagg
      121 ccgtggtgtt cgtgagctgg cacgaggccg cggcattctg ccactggctc tcggagaggg
      181 agggcctgcc gtaccgtctg cccagtgagg ccgagtggga gtacgcctgc cgtgccggca
      241 ccaccacgcc ctactggtgt ggcgacaccc tggctcccgt gttccacaag aaccagcgaa
      301 tgacgtggta cccctcaacg ccgctgcgca ccgacgccgc aaccggactg cccaccctcg
      361 atgtcgtgga cctatcggtg gggcggacgc ccccgaaccc gtgggggctc ttcgacatgc
      421 atgggaacgt cgaggagtgg tgccgcgact ggtacgggcc gtacgcggcg ggggcccaga
      481 ccgacccggc cgggcccgct gggggcgagt tccgcgtcac gcgcggcggg agccattcca
      541 cagagcccta cttcctccga tcatccaacc ggatgggcac attgcccgat gaccggcact
      601 ggctcatcgg cttccgagtg gcgttggggg aagcccccag ggcggtggcg ccgccggcgc
      661 cgaagccgag gcatctgagg cgggtcgatc cggcccccgc ccggtggcgg ccggtgagtg
      721 cggacgcgcc ggtgttccgc ggtcccacgc cctacgtccg tccaccggcg ggcggcgcgc
      781 cgtggatgct cgggcacaac cacgacccgg cactgaccgt gtgcccgaac ggcgacctcc
      841 tggccgtatg gtactcgtgc gccgaggaga ccgggcgcga gctcaagcag ctggcgagcc
      901 gcctgaggcg aggccagacc gagtgggagc ccgcctcggt gttcttcgcg ccgcccgacc
      961 gcaacgccca ctgcccggcg ctgtggtgcg accgccaggg ggtgctccac catttcgcgg
     1021 gcctgtcgat cgcctcgacc tggggcgctc tggccggcgt gatgcgcacg tcggaggaca
     1081 gcggcgccac gtggtcgccg gcgcggctca tcatgcccga gcacggcgcc cggcacatgc
     1141 cggtggcggc caccgagctc tcggatggca ctctggcgct gacctgcgat gcggtgacgg
     1201 gcggcacggg cgccacggcg ctccacctga gtgccgacgg cggccgcacg tggacggatg
     1261 cgggcggcaa cattctgggc atccacgcca gcgtggtcga gctcgccgac ggctcgctca
     1321 tggccctggg gcgcggcgac accgtcgacg gccgcatggc gcggagcctc tcgcgcgacc
     1381 gtggccgcac gtggtcgtac tcggccagtc ccttcccgcc gctgagcagc ggccagcggt
     1441 tggcgctgat ccgtctgcgt gagggcccgt tgttcctggc atcattcgcc aaggggatgc
     1501 ccttggtcga ctcggcgggg gagacacgcc gcgtctcggg cctgttcgcc gcgctgtcct
     1561 tcggcgacgg tgccacctgg ccatgcaccc gcctggtcgc cgagtctggc gagacccggc
     1621 tgggcgagac caccgatggt cagccctgcg ccctggggca cttcgatgcc gagcccatcg
     1681 gctatctgtc cgcttgtcag gcgcctgatg ggctgatcca cctcatcagc agccggaacc
     1741 actacgccat gaacctggcg tggctccggc agggccagcc cccactgccc cccgccctgc
     1801 gccccggggc gctggaggcc cggacggccc tgagccacgc cgtgtcgcca cctcaccttc
     1861 cggccgcc
//
LOCUS       DAOSPL010001054         1866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_62372_length_1866_cov_5.268912, whole genome shotgun sequence.
ACCESSION   DAOSPL010001054 DAOSPL010000000
VERSION     DAOSPL010001054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62372_length_1866_cov_5.268912"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PLQ54_16655"
     CDS             complement(<1..663)
                     /locus_tag="PLQ54_16655"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HOS94950.1"
                     /translation="MEHEPRAEGLNVSPNGSAVLAGPTRTITPSQALNALKGWLPTVA
                     PLSWGVGLVVTAGLIACFREPIARMVEDWRGYVDYHHCAFVPLISAWYCWRRLHNLAT
                     VERTPSAWGLVPLGMGLAMRWVGQIIDVQAMNYAALILVVFGLAWSYLGTQMIRELRF
                     PIGFLIFMIPIPFPILAGAGFPLQTASTKGVYWICKFLGLPIIRDGFSLMVYDFRAVV
                     VEK"
     gene            complement(761..1513)
                     /locus_tag="PLQ54_16660"
     CDS             complement(761..1513)
                     /locus_tag="PLQ54_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS94951.1"
                     /translation="MMPDPVAPYLSVVIPAYNEAQRIGPSLTHVLEYLQAQTYSAEVV
                     IVDDGSTDDTVQVVEAQAQISPLPLRLLRNEGNRGKGYSVRHGMLAASGQLALFSDAD
                     LSTPIEELERLLVPIEAGEAEIAIASRGLAESVLEKRQPIYREMMGRTFNKIVRVLAV
                     PGIADTQCGFKLFTRQAVERVFPYQCTEGFGFDVELLFLARRRGLRIAEIPVRWIDSP
                     ASKVHPLKDATGMFLDVLRVRTNVWRGRYREK"
BASE COUNT          360 a          587 c          621 g          298 t
ORIGIN      
        1 cttctcgacc accacggccc gaaagtcata gaccatcagg gagaagccgt cgcggatgat
       61 gggcagtccc aggaatttgc agatccagta gacgcccttg gtcgacgcgg tctgcaaggg
      121 aaagccagct ccggccagga tgggaaacgg gatcgggatc atgaagatca ggaagccgat
      181 ggggaaccgg agctctcgaa tcatctgggt gccgagatag ctccaggcaa gcccgaacac
      241 caccaggatc agggcggcgt agttcatggc ctgaacgtcg atgatctggc ccacccagcg
      301 catggccagg cccatcccga gaggaaccag gccccaggcg gatggtgtcc gttccacggt
      361 ggccaggttg tgtaaccggc gccagcagta ccatgcggag atcagcggaa cgaacgcgca
      421 gtggtgataa tcgacgtagc cccgccagtc ctccaccatc cgggcgatgg gctcccggaa
      481 gcatgcgatc agaccggcgg tgacgaccag gccgacaccc caggacagcg gcgcgacggt
      541 aggcaaccac cccttgagcg cgttcagagc ttgggaggga gtgatggtac gcgtcggacc
      601 ggccagtacg gcgctgccgt tgggcgatac gttcagacct tccgcccgcg gctcatgctc
      661 catggcgcat cgtgccctct ccgagcgctg gtgtggggaa agggtaccgg cagccccgta
      721 aggccgcaag ggtgacccgg ccccggtctc ttcgctaggc tcacttctct cgataccggc
      781 cccgccatac attggttcgc acgcggagta catccaggaa catccccgtg gcgtctttga
      841 gtggatgcac cttggatgcc ggcgagtcga tccagcgcac ggggatctcc gcaatgcgga
      901 ggccccggcg ccgggccagg aagagcaact cgacatcgaa accaaaaccc tcggtgcact
      961 ggtaggggaa gacccgctcc accgcttgcc gcgtgaacag tttgaacccg cactgggtgt
     1021 cggcaatgcc ggggaccgcc agaacgcgca cgatcttgtt gaacgtccgt cccatcatct
     1081 cccgatagat gggttgtcgc ttctcgagga ccgactcggc cagaccccgt gacgcgatgg
     1141 cgatctctgc ctccccggcc tcgatgggca ccagcagccg ttcgagctcc tcgatgggtg
     1201 tcgacaggtc ggcgtcggag aacagcgcga gctggcccga cgcggcgagc atgccgtgcc
     1261 ggaccgaata ccccttgccg cggtttccct cgtttcgcag gaggcggagg ggaaggggtg
     1321 agatctgggc ctgggcctcg accacctgca ccgtgtcgtc ggtggacccg tcgtccacga
     1381 tcactacctc tgcagagtag gtctgtgcct ggagatactc cagcacgtga gtgagggagg
     1441 gaccgatgcg ctgggcctcg ttgtaggcgg ggatgaccac tgaaagataa ggggcaaccg
     1501 ggtcaggcat catgaagccc ctccttgggg cctgggcgtg tccgttcagg acccgcgggg
     1561 cccgagtcac atatcggcct cccacgtaag ggtatcagcg atggcagtgc cacgggggtt
     1621 cggagcgatg gacgaggacc cggatcggcg gaggctgccg tacaagcaag aagccctgct
     1681 gtcgggcagg gcttccgctg tcaacgatcc ctgggtgaag ccgtgaccag tagggtgttc
     1741 aactcatgtg ccggcggacc cggcggataa ccagcccggc cacactacac agcagcagtg
     1801 ccgcggtcgc gggctcaggc gtccgatgca ccgtgatcga gtcgttcaag cacgactcgc
     1861 cccagg
//
LOCUS       DAOSPL010001055         1864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_62484_length_1864_cov_2.547816, whole genome shotgun sequence.
ACCESSION   DAOSPL010001055 DAOSPL010000000
VERSION     DAOSPL010001055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62484_length_1864_cov_2.547816"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="PLQ54_16665"
     CDS             <1..944
                     /locus_tag="PLQ54_16665"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOS94952.1"
                     /translation="PVGLRHAIGAGIGMLIALVGLHDAGLAVPGRGTLMALGDLSEPT
                     VVVALVGLAVTSVLMARRVPGAMLLGMAASAVLCYATGLAAAPSSALDLPPSVGPTLG
                     QLRMPNILVRPESIIVILTFLYLDMFDTVGTLIGVAQQAGFMRDGQLPRAGRALAADA
                     ASTVVGAVVGTSTVTSYVESAAGVASGGRTGLANMVTAALFLLALFTAPLVRALGGGI
                     ALPDGTIVHPITAPALVIVGAMMMGATRHIDWASDGVPAFLTILVMVVDLNISAGISV
                     GFLSYVLLKVARGQWRQIHGLTYACAAFGTLYFAFLR"
     gene            988..>1864
                     /locus_tag="PLQ54_16670"
     CDS             988..>1864
                     /locus_tag="PLQ54_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94953.1"
                     /translation="MRPIDTPRAIMAAAYCLLALLPVVAQDADGVLRVEVTSQSSPVY
                     LAGEGSATVLVSVHNPTGLQGLADLRVRAKTFSGDLVVRQHGQVKLDGQATVSREVQV
                     PLSALGPVEVTARASVGDVSASDALTLSVLPPAPRSGLTPESAFGINGHIPSLEELRM
                     MQRAGIKWCRCDWLWGIQQPGPGAFAFERFDQLVADARECGISLLPILCYGVEWASTK
                     PAGADGDAANYAPKLEPWLAYVRENVARYKDSITYWEVWNEPNIGFWLSTDDEYVELL
                     KATYSAAKEANPECQV"
BASE COUNT          277 a          595 c          651 g          341 t
ORIGIN      
        1 tcccggtggg ccttcgccat gccatcggag cgggcatcgg tatgctcatc gcgctggtgg
       61 gattacacga tgctggcctc gccgtgcccg ggcgaggcac gctgatggcc ctgggagatc
      121 tgtccgagcc caccgtggtg gtggccctag tcgggttggc ggtgacctcg gtcctgatgg
      181 cccgccgtgt gccgggtgcc atgctactcg gcatggcggc atcggcggtg ttgtgctacg
      241 ccaccggcct ggcggctgcc ccctcctcgg cactggacct gccgccgtcg gtggggccaa
      301 cgctaggcca gctccgcatg cctaacattc tggtgcgacc cgagtcgatt atcgttatcc
      361 tcactttcct ctatctggac atgttcgaca cggtcggcac actcattggg gtggcacagc
      421 aggccggttt catgcgagac ggccagctgc cgcgggctgg acgggcgctg gcggccgatg
      481 cggcatcaac agtggtgggt gccgtcgtgg gcacgtcgac ggtgacgagc tacgtcgaga
      541 gcgccgccgg ggtggcatcg ggcgggcgca ccgggctggc caacatggtc accgccgctc
      601 tgtttctcct ggcgctgttc accgccccgc tggtacgggc cctgggcggc ggaatcgctt
      661 tgcccgacgg taccatcgtg catccgatta ccgcgcccgc tctcgtcatc gtcggtgcga
      721 tgatgatggg cgccacccgc cacatcgact gggccagcga cggtgtgccg gccttcctga
      781 cgatccttgt gatggtggtc gacctgaaca tcagcgcggg gatctcggta ggcttcctga
      841 gctatgtcct gctgaaggtg gcgcgggggc agtggcgcca gatccacggg ttgacctatg
      901 cgtgcgcggc cttcggcacg ctctacttcg ccttcctgcg ctgagaagcc ccacggggct
      961 tggtggcgcc accatggggg tgcttggatg agaccgatcg atacgccgcg ggccataatg
     1021 gccgcagcct actgcctact cgcgctgttg cccgtggtgg cccaggacgc ggacggagtt
     1081 ctgagagtcg aggtgaccag ccagagctcg ccagtctacc tggcgggcga ggggagcgcc
     1141 acagtcctgg tcagcgtaca caatccgacc ggccttcagg ggttggccga tcttcgggtc
     1201 cgagccaaga cattctcggg agacctggtc gtccgtcagc atggccaggt gaagctggac
     1261 gggcaggcta cggtgtcgcg ggaggttcag gtgcccttgt cggccctggg accggtggag
     1321 gtgaccgcca gggcgtccgt cggcgatgtc tcggcctcag acgccctgac cctgagcgtg
     1381 ctgccgccag cgccgcgatc cgggctcacg cccgaatcgg ccttcgggat caacgggcac
     1441 atccccagcc tcgaggagct ccggatgatg cagcgcgcgg gcatcaagtg gtgccgctgt
     1501 gactggctgt ggggtatcca gcagcccggg ccgggtgcct tcgcgttcga gcggttcgac
     1561 cagctggtcg ccgatgcccg cgagtgcggc atcagcctgc ttcccattct gtgctacggg
     1621 gttgaatggg catcgacgaa gcccgcggga gccgacggcg atgccgcgaa ctacgcgccc
     1681 aagctcgagc cgtggctggc atacgtacgg gagaacgtgg cgcgctacaa agactcgatt
     1741 acttactggg aagtctggaa cgagccaaac atcgggttct ggctctcgac cgatgacgag
     1801 tacgtggagc tgctgaaagc cacttacagc gccgccaagg aagccaaccc cgagtgccag
     1861 gtgc
//
LOCUS       DAOSPL010001056         1863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_62520_length_1863_cov_2.809181, whole genome shotgun sequence.
ACCESSION   DAOSPL010001056 DAOSPL010000000
VERSION     DAOSPL010001056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62520_length_1863_cov_2.809181"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1030
                     /locus_tag="PLQ54_16675"
     CDS             62..1030
                     /locus_tag="PLQ54_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS94954.1"
                     /translation="MSLPLLEMHDIAKWFPITSGLLQLHTGDIRAVDGVSLTVHRGET
                     LGLVGESGCGKSTLGRVAVKLMEPTRGHVRFDGQPVDRLKGMELMAFRRRAQIVFQDP
                     YGSLNRRMTAGAIVGEGLHIHRLARRSQVRDRAAELLQRVGLADTDVARYPHEFSGGQ
                     RQRIGIARALAVSPELIVCDEPVSALDVSIQAQIIELLRDLQRELNLSYLFIAHDLAV
                     VRHISHRVAVMYLGRLCEEAPRDVLYDEPLHPYTRVLLAAVPEPDPKVPLRPQALPGE
                     VPSPANPPPGCRFSPRCPVAIDVCRREQPQWRELAPGHFVACHVAG"
     gene            complement(1164..>1863)
                     /locus_tag="PLQ54_16680"
     CDS             complement(1164..>1863)
                     /locus_tag="PLQ54_16680"
                     /inference="COORDINATES: protein motif:HMM:NF036773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOS94955.1"
                     /translation="EFVRYRVVFPSSIAEGVATHEIPFGAIERPVGIEFPAQNWMDWS
                     DGEKGLALLNRGLPGNAVIDGALVLSLLRSTQIVAYGFGGGYEPGMTSATGLERGKRL
                     AFDYSLVPHEGDWRQAGVFHHGQALNQPLIARTAAPHTGPLPTRWGWAQVEPRHVVVS
                     ALRTAPDGAAVLRLYEASGRATPGVRVRLPEGTTRVERANLLEEATGELPVADGAVTF
                     DLRPFEIATLRVVR"
BASE COUNT          272 a          680 c          659 g          252 t
ORIGIN      
        1 agcccgggca cctctcgcgc tgccacttcg ccgggcaact cgaccacggg ggggcgccac
       61 ggtgagcctg cctctgctcg agatgcacga cattgccaag tggttcccga tcacgtcggg
      121 cctcctgcag cttcacaccg gggacattcg cgcggtggac ggcgtctcgc tgacggtcca
      181 ccggggggag accttgggcc tggtcggcga gagcggatgt gggaagtcca ccctgggccg
      241 ggtggcggtg aagctcatgg agcccacgcg cgggcacgtg cggttcgacg gccagccggt
      301 ggaccggctg aagggcatgg agctgatggc cttccgccgg cgcgcgcaga tcgtgttcca
      361 ggacccctac ggctcgctga accgccgcat gaccgccggc gccatcgtgg gcgagggact
      421 gcacatccac cggctggccc gacggagcca agtccgcgac cgggcggcgg agctgctaca
      481 gcgggtcggt ctggccgaca cggacgtggc ccgctatccg cacgagttca gcggcggcca
      541 acgccagcgg atcggcatcg cccgagcgct ggccgtgagc cccgagctca tcgtgtgcga
      601 cgagcccgtc agcgcactcg acgtgtccat ccaggctcaa atcatcgagc tgctgcgcga
      661 cctgcagcgc gagctgaacc tgtcgtacct gttcatcgcg cacgatctgg cggtggtccg
      721 gcacatcagc caccgggtgg ccgtgatgta cctgggtcgt ctgtgcgagg aagcgccgcg
      781 cgacgtgctc tacgacgagc cgctgcaccc ctacacccgg gtccttctcg ccgctgtgcc
      841 cgagccggac ccgaaggtac ccctgcggcc gcaggccctc cccggcgagg tccccagtcc
      901 ggccaaccca ccgcccggct gccggttctc gccgcgctgc ccggtggcca tcgacgtctg
      961 ccggcgcgag cagccccagt ggcgcgagct ggccccgggg cacttcgtcg cgtgccacgt
     1021 ggcggggtag gggaagcgcg gcgacccttg ggccggatag ggtcccaccg gccttcgcca
     1081 ggttccggca cacctccgag caaccgagga ggggccgaat ggaaggggcc cgcgcggcgg
     1141 gaagggcgcc atccggcccg ccttcaccgc accacccgca gcgtcgcgat ctcaaacggc
     1201 cgcaggtcga atgtcaccgc gccgtcggcc accggcagct cgcccgtggc ctcctcgagc
     1261 aggttcgccc gctcgacgcg agtggtcccc tcgggcagcc tgacccgcac gcccggggtg
     1321 gcgcggcccg aggcctcgta gaggcgcagc accgccgcac cgtcgggcgc ggtcctgaga
     1381 gctgacacga ccacatggcg gggttccacc tgggcccagc cccaacgcgt cggcagcggg
     1441 cccgtgtgag gggcggcggt ccgagcaatc agcggctggt tgagggcctg cccgtggtgg
     1501 aacacgccgg cctggcgcca gtcgccctcg tggggcacga gcgagtagtc gaacgccagc
     1561 cgcttgccgc gctcgagccc ggtggcgctg gtcatgcccg gctcgtagcc gccgccaaag
     1621 ccgtaggcga cgatctgcgt gctccgcagg agggacagga ccagagcgcc gtcgatgact
     1681 gcattgccgg gcaggccgcg gttcagcagc gccagaccct tctcgccatc gctccagtcc
     1741 atccagttct gggccgggaa ctcgatgccc acgggccgct cgatggcgcc gaaggggatc
     1801 tcgtgcgtcg ccacaccctc agcgatcgac gagggaaaga cgacgcggta ccgcacgaac
     1861 tcg
//
LOCUS       DAOSPL010001057         1863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_62522_length_1863_cov_2.737832, whole genome shotgun sequence.
ACCESSION   DAOSPL010001057 DAOSPL010000000
VERSION     DAOSPL010001057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62522_length_1863_cov_2.737832"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1863)
                     /locus_tag="PLQ54_16685"
     CDS             complement(<1..>1863)
                     /locus_tag="PLQ54_16685"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOS94956.1"
                     /translation="NAAKLVLGLAFTHTTGIPTGDHDVWVDEIGTAAMTPEAAELSRL
                     VSETRAPQLEGISPLYKIYEAVDIDELRADPRQVIWSLARLPEAEACQAVHPRPQGTG
                     FEKGRRWRFVPLVRAVRNGDELAGFAAALIINGAEPFAGGCWLSMPAPDAGYFGDPRV
                     SRELARLAERMIDGVFLYEGGSQYYVYEDGEPIHLGAQVVGFGRGEARCRVRVEVLSA
                     RAETPLFSRDAQVHVRPGDRRVVDWTWQPGILEGADYRVRVTLERDGAVTDRLEHPLT
                     LWRPHAPAKFVTVKGGDFILDGKQWYPHGVNYMPSSGVGIEDGPYFEFFLDPQPYDPD
                     IIEWDLTACEEIGMNMVSVFIYYRSLDSRNLWDLLTRCRRHGLKVNLSLRPGTPMDFR
                     WDEMRALIEKHKLAECDDVFAYDLAWEPFFGPYAARSVWDPQWEQWVQARYGSVEAAE
                     KAWGFPIPRNADGKVTGPSDTQVAADGEWLAMVLDYRRFVNGLVHDKYAEARRLVRSV
                     DPHHLVSFRMTEAGNPTCGQGWMPYDLQALAGAVDIFAPEGYGRIGDWEAVKPGWFEA
                     AYARACDPELPIMWAEFGYSVWDLQAMRDKPEALGFQGQFYRDFYEMVLKSGANG"
BASE COUNT          331 a          657 c          579 g          296 t
ORIGIN      
        1 ccattggcac cggacttcag caccatctcg tagaagtcac ggtagaactg gccctggaac
       61 ccgagcgcct caggcttgtc gcgcatcgcc tgcaggtccc agaccgagta gccaaactcg
      121 gcccacatga tcggcagttc gggatcacac gcccgggcgt aggccgcctc gaaccagccc
      181 ggtttgacgg cctcccagtc gccgatccgg ccatagccct cgggggcgaa tatgtccacc
      241 gcgcccgcga gggcctggag gtcgtagggc atccatccct gaccgcaggt tgggttcccc
      301 gcctcggtca tgcggaagct gaccaagtgg tgtgggtcca ccgaccgcac caggcggcgg
      361 gcctcagcgt acttgtcgtg aactaggcca ttgacgaacc ggcggtagtc caggaccatc
      421 gccagccact caccatcggc ggccacctgc gtgtcactgg ggccggtcac cttcccgtct
      481 gcgttgcgag ggatggggaa accccaggcc ttctcggcgg cctcgaccga gccataccgc
      541 gcttgtaccc actgctccca ctgggggtcc cagaccgacc gcgcggcata gggcccgaaa
      601 aacggctccc aggccaggtc gtaggcgaac acatcatcac actcagccag cttgtgcttc
      661 tcgatcagcg cccgcatctc gtcccatcga aagtccatgg gggtccccgg ccgcagcgag
      721 aggtttacct tcagcccatg ccgccggcag cgggtgagca gatcccacag gttcctggaa
      781 tcgagcgatc ggtagtagat gaacacgctc accatgttca tcccgatctc ctcgcaggcc
      841 gtcaggtccc actcgatgat gtcggggtca tacggctgag ggtcgaggaa gaactcgaag
      901 taggggccgt cctcgatgcc gacaccactc gacggcatgt agttgacgcc gtgggggtac
      961 cactgcttcc cgtcgaggat gaagtcgccg cccttgaccg tcacgaactt cgcgggagcg
     1021 tgcggccgcc atagggtgag cgggtgctcg agccggtcgg tgacggcacc atcgcgttca
     1081 agtgtgacac ggacccgata atcggcaccc tcgaggattc ccggctgcca ggtccagtca
     1141 acgacccggc gatcccctgg cctcacgtgg acctgcgcgt cccggctgaa cagaggcgtc
     1201 tccgccctgg ctgagagcac ctcgacccgg acgcgacagc gcgcttcgcc gcggccgaac
     1261 cccacgacct gcgcccccag gtggattggc tcaccatcct cgtagacgta gtactggctc
     1321 cctccctcgt agagaaacac gccatcgatc attcgctcag ccagacgggc cagctcccgg
     1381 ctgactcgcg ggtcaccgaa gtagcccgca tccggcgcgg gcatcgagag ccagcaaccg
     1441 ccggcgaagg gctcggcgcc gttgatgatg agcgccgcgg cgaaacccgc cagctcatcg
     1501 ccgttccgaa ccgcccgcac caggggaacg aagcgccagc ggcggccctt ctcgaagcca
     1561 gtcccctgcg gtctgggatg aaccgcctgg caagcctcgg cctcaggaag gcgcgccagc
     1621 gaccagatca cctgccgtgg atccgcccgc aactcgtcga tgtccaccgc ctcgtagatc
     1681 ttgtacaggg gcgagatgcc ctcgagctgc ggagccctgg tctccgacac gaggcggctg
     1741 agctcagccg cctcgggggt catggcagcg gtgccaatct catcgaccca gacatcgtgg
     1801 tcgcccgtcg gtatgccggt ggtatgggtg aaggcgagcc cgagcaccag cttggccgcg
     1861 ttc
//
LOCUS       DAOSPL010001058         1862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_62547_length_1862_cov_3.199779, whole genome shotgun sequence.
ACCESSION   DAOSPL010001058 DAOSPL010000000
VERSION     DAOSPL010001058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62547_length_1862_cov_3.199779"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="PLQ54_16690"
     CDS             <1..867
                     /locus_tag="PLQ54_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94957.1"
                     /translation="PWAPQHYGMNVLADFRPGGQAFDWALAANWHARPENRTASGEAD
                     AEAWVPGAFRMLPGVLQWGPAPLMVNGEGRISPALPLYCGLVTVMAPSGAASVRNTAF
                     LPPGDDGEEPGLSLTPAQPHTGGPARTCESCHVPDAPPTGPGRSNPAEPLVVDLRTAD
                     GSLVPRRTRSQLPALPAPTAGAGEMSRRFPLARPLTDAERALIDQRGVCAACHQETPS
                     RFIAPSLLHHVAQATGLRPRTSGQHQTLVRVTLLTAAWAQVLAAVLVPAGLVLWVRRR
                     RSLAGRYRRMVS"
     gene            1001..>1862
                     /locus_tag="PLQ54_16695"
     CDS             1001..>1862
                     /locus_tag="PLQ54_16695"
                     /inference="COORDINATES: protein motif:HMM:NF039329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94958.1"
                     /translation="MPLWPLMRVALPGSVSPREVPVHLLVPFRKGLLQPDQISRLAIW
                     TQDGVTPRAAMTPSPVSYWSDGSIKWLTARARLRLGPPEGPRAVIRPGRVEACEPRVI
                     VSEDDVGYRVETGRLRVSLPTASGFPLGDVWLAGSEADSGEEPRRVIAPGDGVAVEVT
                     TSQGGPYRSVTAPSQCELEHSSPQEAVMRFGGALLDSTGALGAHYTARLVFFAAAPFF
                     MMRLSVEAGGSLADEVTGVSLSLAAAVAVDSVRLEDPADREGFLDVTLESDEWLSLSS
                     SDGAAAVTIGT"
BASE COUNT          244 a          693 c          647 g          278 t
ORIGIN      
        1 ccctgggcgc cccagcacta cgggatgaac gtgttggccg acttccggcc cggcggccaa
       61 gccttcgact gggcgctcgc cgcgaactgg cacgcacggc ccgagaaccg caccgcgtcg
      121 ggtgaggccg atgccgaggc ctgggtgccc ggggcgttcc gcatgcttcc gggtgtgctc
      181 cagtggggtc cggcgcccct gatggtgaac ggcgagggtc gaatctcccc cgcgttgccc
      241 ctctactgcg gcctcgtgac ggtcatggcc ccgagcggcg cggcgtccgt gcggaacacc
      301 gctttccttc cgcccgggga cgatggcgag gaacccgggc taagcctcac cccggcccag
      361 ccccacacgg gcgggcccgc ccggacgtgc gagtcctgcc atgtgccaga cgccccacct
      421 accgggccgg gccgctcgaa cccggcggaa ccgttggtgg tcgatctccg caccgcggac
      481 gggtccctcg tcccccggcg gacgcgttct caactgccgg ctcttcccgc cccaacggcc
      541 ggagcgggag agatgagccg tcggttcccc ctcgcgcggc cactgaccga cgccgagcga
      601 gcgctcatcg atcagcgcgg cgtctgcgcg gcctgccacc aggagactcc ctcacgcttc
      661 atcgcgccca gcctgctgca ccacgtggcc caggccacgg ggctgcgtcc gcgcacgtcg
      721 gggcaacacc agacgctcgt ccgcgtgacg ctccttacag ccgcctgggc ccaggtgttg
      781 gccgccgtcc tggtgcctgc gggactcgtg ctgtgggtgc gccgccgacg gtcactggcc
      841 gggcggtacc ggcgcatggt atcctgaggt gcccccggac cgtgccactg gcgtggcgag
      901 gtgtgtgggg cgcatccatc cccgtggagg agctgtggcg cccggcccag ggtcgccgtc
      961 ccgcatgccc gtggtcgaac ccgagccaag aagcgcgccg gtgccgctgt ggcccctgat
     1021 gcgggtcgcg ctgcccggca gcgtgtcccc gcgggaggtg cccgtccacc tgctggtacc
     1081 cttccggaag ggcctcctcc agcccgatca gatctcgcgc ctcgccatct ggacccagga
     1141 cggcgtcact ccgcgggcag cgatgacacc gtcgccggtc tcatactggt ccgatggatc
     1201 catcaaatgg ctgacggcca gggcgcggct ccggctgggt ccgcccgaag gcccccgggc
     1261 ggtcatccgt ccagggcgcg tcgaggcctg cgagccacga gtgattgtct cggaagacga
     1321 cgtggggtac cgcgtcgaga cgggccgatt gcgggtgtcg ctgccgaccg ccagcgggtt
     1381 ccccctgggt gatgtctggc tcgcgggatc cgaagcggac agcggcgagg aaccgcgccg
     1441 cgtcatcgca ccgggcgacg gggtggccgt cgaggtgacc acctctcaag gcggccccta
     1501 ccgaagcgtg acggcgccca gccaatgcga actcgagcac tcgtcaccac aggaagccgt
     1561 gatgcggttc ggcggcgctc tgctcgatag cacgggagcc ctgggcgccc actacaccgc
     1621 tcgcctggtg ttcttcgctg ccgccccctt cttcatgatg agactgtcgg tcgaggccgg
     1681 cgggtcactg gccgacgagg tgacaggcgt cagcctctcc ctggcagccg ccgtggctgt
     1741 ggatagcgtc cggctcgagg accccgccga ccgcgagggc ttcctcgatg tcaccctcga
     1801 aagcgatgag tggctctccc tctcctccag cgacggcgcg gcggcggtaa ccatcggcac
     1861 cg
//
LOCUS       DAOSPL010001059         1860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_62640_length_1860_cov_2.684211, whole genome shotgun sequence.
ACCESSION   DAOSPL010001059 DAOSPL010000000
VERSION     DAOSPL010001059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62640_length_1860_cov_2.684211"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="PLQ54_16700"
     CDS             <1..1026
                     /locus_tag="PLQ54_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HOS94959.1"
                     /translation="RPGLNLVNTSFAAGVDFWISFSIGIGIALFAYSVISLVRKAMAE
                     KRRGERRMEALFRPPPGRGDLPIYLVVGMFVVATMSYIILCRKLVPGFPLTFLLIYGL
                     IVTPAESLISARMLGVAGQWVGIPMLREGTFILSKYQGVDIWFAPIPLSDLGGTAQYF
                     RMVELTGTRVMSIIKADLVMAPIVLVCGLLFWQFAWKLAPIPSSIYPYTEAQWPLNSL
                     HQTFWITATRPGESPFREAFNYVYSIMGFVVGFGGLSLMGVIGLPITLMYGFIRGIGG
                     LPHSTIPEFTGALLGRYYLERRLGRDTWRRYSVVLLAGYSCGMGLVGLSSAALAMVTT
                     SVSRLQY"
     gene            1028..>1860
                     /locus_tag="PLQ54_16705"
     CDS             1028..>1860
                     /locus_tag="PLQ54_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94960.1"
                     /translation="MGVAEQSHETSLITSDAGLAQVTQTRKSPITARAVLIGLLLMVP
                     NAYWIVMMEVRWQLLDGTCLPLFVTPVFTLLLVSGFNAALSKLAPRARLTAAELVTIY
                     VMQVVVCCFTGHDTIANLIGTIAHKVRNTTLDNRWDELFAGLFPDWMWVNNKELVDEF
                     YQGTSDSLFSPPIIALWQKPLLIWCGFFAMFCATLLSMAAVLRRSWIEHDRLSFPIVQ
                     LPLGITAPGKQTVLSDPLFWTAFGMAAVVSLMNGLNLFYPAVPSFPRVKITDIGRYFQ
                     NP"
BASE COUNT          290 a          609 c          562 g          399 t
ORIGIN      
        1 cgccccgggc tcaaccttgt gaatacctca ttcgcggcgg gcgtcgactt ctggatcagt
       61 ttctccatcg ggatcggcat cgcgctcttc gcctacagcg tcatcagcct ggtgaggaaa
      121 gccatggccg agaagcggcg tggcgaacgg cggatggagg ctctgttccg gccgcctccc
      181 ggtcgaggcg acctccccat ctatctggtc gttggcatgt tcgtcgtggc gacgatgtcg
      241 tacatcattc tctgccggaa gctggtgcct ggcttcccgc tcacgttcct gctgatctac
      301 ggcctcatcg tcaccccagc cgagtcactc atctcggcga ggatgctggg tgtcgctggg
      361 cagtgggtgg gcatcccgat gctgcgcgag ggcaccttca tcctgagcaa gtaccagggc
      421 gtggacatct ggttcgcgcc gattccgctg tcggacttgg gtggcactgc ccagtatttc
      481 cgcatggtcg agctcaccgg aacccgcgtg atgagcatca tcaaagccga tctggtcatg
      541 gcacccattg tgctggtgtg cgggcttctg ttctggcagt tcgcgtggaa gctggcgcca
      601 atcccctcga gcatctaccc atacacggag gcccagtggc cgctgaactc gctgcaccag
      661 accttctgga tcacggccac gcggccgggt gagtcgcctt tccgcgaagc attcaactac
      721 gtgtactcga tcatgggctt cgtggtcggt ttcggcgggc tgagcctaat gggcgtgatc
      781 ggcctgccca tcacactgat gtacgggttc atccgtggga tcggcgggct gccgcactca
      841 acgatccccg agttcaccgg cgcgctgctc ggacgctact acctcgagcg tcggctggga
      901 cgggacacct ggcggcgcta ctccgtggtg ctgctggccg gttacagctg cggcatggga
      961 ctcgttggtc tgtcgtcagc cgcgctggcc atggtcacca cttcggtctc gaggttgcag
     1021 tactgacatg ggtgtggccg aacaatctca tgagacaagc ctaatcacca gcgacgccgg
     1081 cctggcccag gtgacccaga cccggaagtc gccgatcacc gcgcgcgccg tgctgatcgg
     1141 cctcctcctc atggtgccca acgcctactg gatcgtcatg atggaggtcc gctggcagct
     1201 tctggacggc acctgccttc cgctgttcgt cacgccggtg tttaccctgc tgctggtctc
     1261 gggcttcaac gcagctctgt cgaagctggc cccccgagct cggctcaccg ccgcggaact
     1321 cgtaacgatc tatgtgatgc aagtcgtggt gtgctgcttc acggggcatg acaccattgc
     1381 gaacctcatc ggcaccatcg ctcacaaggt ccggaacacg accctggaca atcggtggga
     1441 tgagctattc gccggcctgt tccctgactg gatgtgggtc aacaacaagg aacttgtcga
     1501 cgagttctac cagggcacgt cagactcgct cttctcgccg ccgatcatcg ccctctggca
     1561 gaagccactc ctgatctggt gcgggttctt cgccatgttc tgcgcgacgc tgctctcgat
     1621 ggccgccgtg ctcaggcgtt cgtggatcga gcacgaccgc ctgtcctttc ccatcgttca
     1681 gctgcctctg ggcatcacgg cgccggggaa gcagacggtg ctatcggacc cgctcttctg
     1741 gaccgctttc ggtatggccg ccgtcgtcag cctgatgaac gggctcaacc tgttctaccc
     1801 ggccgtgccc tcgttcccgc gcgtgaagat caccgacatc gggcggtact tccagaaccc
//
LOCUS       DAOSPL010001060         1856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_62817_length_1856_cov_3.012771, whole genome shotgun sequence.
ACCESSION   DAOSPL010001060 DAOSPL010000000
VERSION     DAOSPL010001060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62817_length_1856_cov_3.012771"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="PLQ54_16710"
     CDS             complement(<1..1137)
                     /locus_tag="PLQ54_16710"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014413850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOS94961.1"
                     /translation="MDGAEYRLENDQLGDVPVPGDALYGAHTVRALANFPLARRPVPA
                     VLVRAFGAVKLACAQTNRALGQWSGDPGKADAIVRACSEMLEGLLSEHVVVDLLQGGA
                     GTSMNMNVNEVLANRALDILGEPRGHYARVSPLDDINRQQSTNDTYPTALRVAAIWEL
                     RDLEARLVALQESFQAAERRFAHIVKVGRTQYQDAVLTTLGREMGAYAEAINRDRWRV
                     YKCEERLRVVNLGGTAIGTGLGASREFIFRATDALRQVTGIGVARAENLVEATQNADV
                     FVEVSGILKACAASMLKICTDLRLMSSGPEAGLGELRLPPRQAGSSIMPGKVNPVIPE
                     AVSQVALLVMGYDGVTAMACASGSLELNPFLPLVAFCLLESIQLL"
     gene            complement(1152..>1856)
                     /locus_tag="PLQ54_16715"
     CDS             complement(1152..>1856)
                     /locus_tag="PLQ54_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008685260.1"
                     /note="in Escherichia coli this enzyme functions in
                     thiamine biosynthesis along with thiFSGI and IscS; with
                     ThiFSG catalyzes the formation of thiazole phosphate from
                     tyrosine, cysteine and 1-deoxy-D-xylulose-5-phosphate;
                     forms a complex with ThiG; contains an iron-sulfur center;
                     in Thermotoga this enzyme has an extra C-terminal domain;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOS94962.1"
                     /translation="VGIGVLFGLADWRFEILALMEHSRHLEARFGVGPHTISVPRLEP
                     ATGSHVSLSPPHAVSDDDFRRIVAILRLAVPYTGIIMSTRETPHLRRETFALGVSQIS
                     AGSRTNPGGYSEDDTEADAAQFSLGDHRPLDEVIRDVASLGYIPSFCTACYRLGRTGR
                     DFMDLAKPGEIKAHCDPNALSTFLEYLLDYASPETCAVGEKAIGDILATMPEPMRNHS
                     ERLMSKVREGQRDVFC"
BASE COUNT          344 a          587 c          622 g          303 t
ORIGIN      
        1 cagaagctgg atggattcta gcagacagaa tgcaaccagg ggcagaaagg ggttaagttc
       61 gaggctaccg gatgcgcaag ccatggcagt gaccccatcg tagcccatca ccagcagtgc
      121 tacctgactc acggcttcgg gtataaccgg gttcaccttc cccggcatga tggaggaacc
      181 tgcctgcctc ggaggcagcc ggagttcgcc cagccccgcc tcaggacccg acgacatgag
      241 ccgcaggtcg gtgcagatct tgagcatcga ggcggcacag gccttcagga tgccggagac
      301 ctcaacgaac acgtccgcat tctgggtggc ctcgaccagg ttctcggccc gggcgacgcc
      361 gatacccgtc acctggcgta gcgcatcggt ggcgcgaaag atgaactcac gcgaggcgcc
      421 cagaccggtg ccgatggccg ttccgccaag gttcacgacg cgaaggcgct cctcgcactt
      481 gtagacacgc catcgatcgc ggttgatggc ctcggcgtaa gcccccatct cgcggcccag
      541 agtggtcagt acggcgtcct ggtactgggt tcggcccacc ttcacaatgt gggcgaaacg
      601 cctctcggca gcctggaacg actcctggag ggccaccagc ctggcctcga gatccctcag
      661 ctcccagatg gccgccacgc gaagagccgt ggggtaggtg tcgttggtcg actgctggcg
      721 gttgatgtcg tccaggggcg agactcgggc atagtggccc cggggctcgc ccaggatatc
      781 gagcgcccga ttcgccagga cctcgttcac gttcatgttc atgctggtgc cggcgccgcc
      841 ctggagcagg tcgaccacga catgttcgga gagcaaaccc tcgagcatct ccgagcacgc
      901 gcggacgatg gcgtcggctt taccaggatc gccggaccac tggccgagtg cccggttcgt
      961 ctgcgcgcag gcgagcttga ccgcgccgaa tgctcggacg agcacggccg gaacggggcg
     1021 ccgggcgagg gggaagttgg cgagagcccg gaccgtgtgg gccccataga gagcatcgcc
     1081 cggcacagga acatcgccga gctggtcatt ctcaagccgg tattcggccc cgtccatatc
     1141 gccccgtccg cctaacagaa cacgtcacgc tggccttctc ggactttgga catgaggcgc
     1201 tccgagtggt ttcgcatcgg ttcgggcatc gtggccagga tgtcgccgat ggccttctca
     1261 cccacggcgc aggtctcggg cgatgcgtag tcgagcaggt actcgaggaa tgtcgagagg
     1321 gcgttggggt cacagtgggc cttgatctct ccaggcttgg ccaggtccat gaagtcacgg
     1381 cccgtcctgc cgagccggta gcacgccgtg cagaacgacg ggatgtagcc cagcgacgcc
     1441 acatcgcgga tgacttcgtc cagcgggcga tggtcgccca gggagaactg ggccgcatca
     1501 gcctctgtat cgtcctcgga gtagcccccg gggttcgtgc ggctgccggc cgagatctgc
     1561 gagacgccca aggcgaaagt ctcccggcgc agatggggag tctccctcgt cgacatgatg
     1621 atgcccgtgt aaggaaccgc cagcctgagg atggcaacga tgcgacgaaa gtcgtcatcg
     1681 gagaccgcgt gagggggcga caaggagaca tgcgatcccg tggccggttc gagtcggggc
     1741 acgctaatgg tgtggggtcc gactccgaag cgcgcctcga gatgacggct gtgctccatc
     1801 agggccaaga tctcgaaacg ccagtcggcg aggccgaaga gaacgccgat gcccac
//
LOCUS       DAOSPL010001061         1856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_62828_length_1856_cov_2.672404, whole genome shotgun sequence.
ACCESSION   DAOSPL010001061 DAOSPL010000000
VERSION     DAOSPL010001061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62828_length_1856_cov_2.672404"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PLQ54_16720"
     CDS             <1..566
                     /locus_tag="PLQ54_16720"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOS94963.1"
                     /translation="GLTPGAAEATAHAAGAGSGGYYAASTDEWAQFRSYVNLARHLNP
                     RLSEQEASLIAQCLFAYCARFGVPRPVMASVIYAESRFNPRAKSPAGALGLCQLMPGT
                     AAGMGLSSPYDINQSIYGGTLYLARQMQRFYPRAGQRCLEHALAAYNAGPGAVEEYNG
                     VPPYAETQRYIRTVARMCSELSRLGYR"
     gene            676..1017
                     /locus_tag="PLQ54_16725"
     CDS             676..1017
                     /locus_tag="PLQ54_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94964.1"
                     /translation="MAGHDCSRQVGQAIDNLVKLELVMFFHENPETLDDACGISRRLG
                     YSPEVIESFMPELVAAGLLMQHGQGPGALYRYTQDPEVRRVVEGDYSSRYQTKAERRR
                     LESALAAKGVV"
     gene            1018..>1856
                     /locus_tag="PLQ54_16730"
     CDS             1018..>1856
                     /locus_tag="PLQ54_16730"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOS94965.1"
                     /translation="MARAKLGVAGLDAILHGGVVPGNAILVEGTPGAGKTTLGMQFIY
                     AGITQHNEPGLIVTFEEFPSQMYRDAGMLGWDLQALANENKLKIISTSPAVFRQETAG
                     PSGLITQMAREIGAERVMVDSISHFQRLTQDPAEQREIFNSLVNMLKRGGFTAMLTKE
                     VKSRSGDEVSFEEYASDTALRLTYEVEASTQRARYVEVVKARGQDFIPGKHSFLIGRG
                     GITVFPSVALSTDDDAAKAHIERIDSGIPGLDEMLHGGLIRAWSLLLVGPAGSGKTIF
                     GLQS"
BASE COUNT          308 a          552 c          662 g          334 t
ORIGIN      
        1 ccggcctcac gccgggcgcc gcggaagcga cggcccatgc ggcgggtgca ggaagtgggg
       61 ggtactacgc cgcctccacg gacgagtggg ctcagttccg atcgtatgtc aacctggcgc
      121 ggcacctgaa cccgaggctg agcgaacaag aggcgagcct gatcgctcag tgcctgttcg
      181 cctactgtgc tcgtttcggg gtgccccgcc ccgtcatggc cagcgtgatc tacgcggagt
      241 cgcggtttaa ccctcgggcc aagtcgccgg cgggcgcgct ggggctttgc cagctcatgc
      301 ccggtaccgc cgccggcatg ggcctgagct caccgtatga catcaaccag agcatctatg
      361 gcgggacgct ctatctggct cgtcagatgc agcgcttcta cccccgcgcc ggccagcggt
      421 gcctcgagca cgctctggcg gcgtacaatg cgggtcccgg agccgtcgag gagtacaatg
      481 gcgtgccgcc ctacgccgag acacagcggt acattcgcac ggtggcccgc atgtgctccg
      541 agttgtcgcg cctgggctat cgctagggcg ccgacgggca agggattccc tgcatcgcct
      601 ccgaatagcc gggatggtac agcgtggctc gtgcctgggc ccgcgcactc gtgcgcaggg
      661 gctggggaga tgatcatggc gggtcatgat tgctcccgtc aggttgggca agccatcgac
      721 aacctggtga agttggagct cgtgatgttc tttcacgaga acccggagac gctcgatgac
      781 gcctgcggca tctcgcggcg cctcgggtat tcgcccgagg tgatcgagtc gttcatgccc
      841 gagctggtgg ctgctgggct cctgatgcag catgggcagg ggcccggggc gttgtaccgt
      901 tacactcagg accccgaggt gcgccgagtg gtggaggggg actactcgtc acgctaccag
      961 accaaagcgg agcggaggcg cctcgagtcg gctttggcgg cgaagggcgt agtgtagatg
     1021 gcgagagcga agctcggggt agcgggactc gacgcgatcc tccacggcgg cgtcgtgccg
     1081 ggcaatgcca tcctggtgga ggggacgccc ggggccggca agactacgct gggtatgcag
     1141 ttcatctatg cggggatcac ccagcacaat gagcccggcc tgatcgtcac gttcgaggag
     1201 tttcccagcc agatgtaccg tgatgcgggc atgctgggtt gggatcttca ggcactcgcc
     1261 aatgagaaca agctgaagat catctcgacg tcgccggcgg tgttccgtca ggagacggcg
     1321 gggcccagtg gtctgatcac gcagatggct cgcgagatcg gcgccgaacg ggtgatggtg
     1381 gattcgatct cgcatttcca gcggctgacc caggacccgg cggagcagcg tgagatcttc
     1441 aactcgttgg tgaacatgct caagcggggc gggttcacgg ccatgttgac caaggaggtc
     1501 aagtcgcgct cgggcgacga ggtttcgttc gaggagtatg cgtcggacac ggcgcttcgc
     1561 ctgacctacg aggtggaggc atcgacgcag cgggcccgtt atgtggaggt tgtgaaggcg
     1621 cggggacagg acttcatccc gggcaagcac agcttcctga taggccgcgg aggcatcacg
     1681 gtgtttccgt cggtggcgct ttcgaccgac gatgacgcgg cgaaggccca catcgagcgg
     1741 attgactccg ggataccggg gttggatgag atgctccacg ggggactcat ccgcgcctgg
     1801 tcgctgttgc tcgttggccc ggccggctcg ggcaagacca tcttcggtct gcagtc
//
LOCUS       DAOSPL010001062         1855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_62878_length_1855_cov_2.973333, whole genome shotgun sequence.
ACCESSION   DAOSPL010001062 DAOSPL010000000
VERSION     DAOSPL010001062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62878_length_1855_cov_2.973333"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1366
                     /locus_tag="PLQ54_16735"
     CDS             179..1366
                     /locus_tag="PLQ54_16735"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOS94966.1"
                     /translation="MAMSLIVCLCLPTCGWAVAAPAEQPTDVDEEAILARSLVSIGDT
                     ARLQHALAKARRGQPVVVGVIGGSITQGASASTEEGRYGNRVAEWWRTRFPGTRVTFI
                     NAGVGATGSDTGAHRAQAHLLDHRPDFVVAEYAVNDPNTEFAAETLEGLTRQILTQPS
                     QPALMLLFTMNNAGANAQEWHSKVGEHYGLPMVSFRDALWPEIRDGRMKWEDIEADAV
                     HPNDRGHAYCAQFICRVLDTVMAGLPDDADLPPVPPVPAPLISDVFQHATFYNFDLLT
                     PVVCEGFEAHEAWPFGTGWQAEAAGSRIEFELEGSAISVSFFRIKGAMGQAEAQVDDR
                     EPVRLEAWFDQDWGGYSAFQLIARDLGPGKHRLRIRVLDEKAPASEGNRFLIHAIMAA
                     GLR"
     gene            complement(1409..>1855)
                     /locus_tag="PLQ54_16740"
     CDS             complement(1409..>1855)
                     /locus_tag="PLQ54_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94967.1"
                     /translation="GGMGGPAATAEAASRLDRAMRARMGGYAISQRQNALNLRGQAQA
                     PRNEWIDAEGQVQPIGGVRYVAGRAFYNRNGVWVDARYRPEQRVMNVQALSEAQFQLG
                     RAMPALNQYFSIGEHVLVEVNGQAVMLGPEGRAELTEADLADLMRG"
BASE COUNT          294 a          634 c          639 g          288 t
ORIGIN      
        1 cgcccgccgg ccgagccgat tgtgggtgtc cctgtccgag gggacaggcg cttgtcggcg
       61 gaaagacagg tatgacctgc cccgtggtcc gctgagaccg agaagccggc tcgcctgacc
      121 gggctggggc ctggcgcgcg agggcagagc gcacgaacca cgtgacggat gggatgagat
      181 ggcgatgtcg ctgatcgtgt gtctgtgtct gccgacctgt gggtgggccg tggccgcacc
      241 cgccgagcag ccaactgatg tcgatgagga ggcgatcttg gcccgctcgc tcgtgagcat
      301 cggtgacacc gcccgcctgc aacatgctct ggcgaaggcg cgccgcggcc agcctgtggt
      361 cgtcggggtc atcgggggat ccatcaccca aggcgcgtcg gcctccaccg aggaaggccg
      421 gtacgggaac cgggtggccg aatggtggcg gacccggttc ccggggactc gcgtcacatt
      481 catcaacgcc ggagtcggcg ccacggggtc ggacaccggc gcccaccggg ctcaggcgca
      541 tctgctcgac caccggcccg acttcgtcgt ggccgagtac gctgtcaacg acccaaacac
      601 cgagttcgcg gcggagacgc tcgagggact gacccggcag atcctcacgc agcccagtca
      661 gcccgccctg atgctgctgt tcaccatgaa caatgccggg gccaatgccc aggagtggca
      721 ctcgaaggtc ggcgagcact acggcctgcc gatggtgagc tttcgcgacg cgctgtggcc
      781 ggagatccga gacggacgga tgaagtggga agacatcgag gccgacgccg tccatccgaa
      841 cgacaggggg catgcctact gcgcccagtt catctgccgc gtcctcgaca cggtgatggc
      901 cggcctaccg gacgatgcgg accttcctcc cgtgcctccg gtccccgccc cgctcatcag
      961 cgacgtcttc cagcacgcaa cgttttacaa cttcgacttg ctgacgccgg tggtgtgcga
     1021 gggcttcgag gcccacgagg cgtggccctt cgggacggga tggcaggccg aagctgcggg
     1081 cagccggatc gagttcgagc tggagggctc ggccatcagt gtgtcattct tccgcatcaa
     1141 gggcgccatg ggccaggcag aggctcaggt cgacgaccgc gagccggtca ggctcgaagc
     1201 gtggttcgac caggactggg gaggctactc ggcctttcag ctcatcgcgc gcgaccttgg
     1261 accggggaag caccggctcc gcatccgcgt gctcgacgag aaagcacccg ccagcgaagg
     1321 taatcggttt ctcatccacg ccattatggc ggcggggctg agatagcgcg gcggggccgg
     1381 cggcgcaggc tgccggcccc gccacaagct acccgcgcat caggtcggcc aagtcggctt
     1441 cggtgagctc ggcgcgaccc tcggggccga gcatcacggc ctggccgttc acctcgacca
     1501 agacgtgctc gccgatgctg aaatactggt tgagcgcggg catggcccgt ccgagctgga
     1561 actgagcctc actcagcgcc tgcacgttca tcacacgctg ctcgggccgg tagcgcgcat
     1621 cgacccacac cccgttccgg ttgtagaagg cgcggcccgc cacgtagcgc accccgccga
     1681 tcggctggac ctgcccctcg gcatcgatcc actcgttgcg gggcgcctgg gcttggcctc
     1741 gtaggttcag ggcattctgc ctctgcgaga tggcgtaccc gcccatgcgg gcccgcatag
     1801 cgcggtccag gcgactggcg gcctctgccg tggccgcggg cccccccatc ccgcc
//
LOCUS       DAOSPL010001063         1853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_62967_length_1853_cov_3.395439, whole genome shotgun sequence.
ACCESSION   DAOSPL010001063 DAOSPL010000000
VERSION     DAOSPL010001063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62967_length_1853_cov_3.395439"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..366
                     /locus_tag="PLQ54_16745"
     CDS             85..366
                     /locus_tag="PLQ54_16745"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOS94968.1"
                     /translation="MTIGVLRVTLRIPEAHSLKDKRRVVKSLLDRVANQFNAAVAEVG
                     DLDVRTRAELAVVTVSNDRRHANAMLDSIRNFVDAHAAAEVASADIELL"
     gene            435..839
                     /gene="rbfA"
                     /locus_tag="PLQ54_16750"
     CDS             435..839
                     /gene="rbfA"
                     /locus_tag="PLQ54_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOS94969.1"
                     /translation="MSATMTVRQQQVARMLVAEISDIIRREMNDPRLGFATLVGAEPS
                     RDLRHARVYVSVLGDEEDQRRSLEALNGAVGYIRSLLGPRLTMRYVPEISFRLDHTAE
                     SAQHIEALLRDLPRAQAEPMGNARADDASTDR"
     gene            817..1812
                     /locus_tag="PLQ54_16755"
     CDS             817..1812
                     /locus_tag="PLQ54_16755"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOS94970.1"
                     /translation="MTRPRIADAARVLAEGQSLLVTTHIRPDGDAIGSALALSHGLRG
                     LGKMATIVLEHAVPRPYRFLPGWRDIVPPLPVSEAGRFDVAVVLDCDGLQRAGQVTGL
                     VRAVGRIVEIDHHPGAHGDGCVIAVYPEAAATAELVFELCEALGAPLTVELATCLLTG
                     LTDDTGGFRHSNTTARAHELASRLVAAGADPARIAENLFGNQPPARLVLRGRALARAR
                     LLLDGRVILSYLTNKDFAVTGADRADSSYVIDDLRLAADIQVAAVLVETENGQCRVSL
                     RSSSDLDVAAVARSLGGGGHVKAAGCELPGTVAQAEPPLLAALAGALGADGRHGG"
BASE COUNT          281 a          606 c          678 g          288 t
ORIGIN      
        1 ggcgatatca tcgaggtcca caccatggag cgcgtcacgc gcacgcttca gagctagcgg
       61 cgtggtggcg gcggggctcg ggccatgacc atcggggtgc tccgagtgac gctccggatc
      121 cctgaggcgc actcgctcaa ggacaagcgt cgcgtcgtca agagcctgct ggatcgggtg
      181 gcgaaccagt tcaacgcggc cgtggccgag gtgggcgacc tcgacgtgcg gacccgggcc
      241 gaactggcgg tcgtcacggt gagcaatgac cggcgtcacg ccaatgccat gctggactcg
      301 atccgcaact tcgtggatgc ccatgcggcg gcggaggttg cctcggcgga catcgagctg
      361 ctctgaacat cgcggtgtgg atctcatggg caggaccggg acgcgtgacc cgggagcctg
      421 ggggtgagtg ggcgatgtcc gcgaccatga ccgttcggca gcagcaagtc gcgcgaatgc
      481 tggtggcaga gatcagcgac atcatccgcc gcgagatgaa cgatccacgt ctggggttcg
      541 ccaccctcgt gggggccgag ccctcccgcg acctcagaca tgcgcgagtg tatgtgagcg
      601 ttctgggtga tgaagaggac cagaggcgaa gcctcgaggc cctcaatggc gccgtggggt
      661 acatccgctc cctgcttgga ccccggttga ccatgcgcta cgttccagag ataagcttcc
      721 gcctggacca cacggcggag tccgcgcagc acatcgaggc gctccttcgg gaccttcctc
      781 gcgcgcaagc ggagcccatg gggaacgcga gggccgatga cgcgtccacg gatcgctgac
      841 gccgcccgag tcctcgccga gggacagtcg ctcctcgtga cgacccatat ccggccagac
      901 ggggacgcca tcggctcagc cctggcgctt tcccacggac tgcgggggct cgggaagatg
      961 gccacgatcg tgctcgagca cgcagttccc cggccctacc gtttcctgcc aggctggaga
     1021 gacatcgtgc cgccgctccc cgtatcggag gcgggccggt tcgatgtggc cgtggtgctg
     1081 gactgtgacg gcctgcagcg ggccggccag gtcaccggcc tggtccgggc cgtggggcgg
     1141 atcgtcgaga tcgaccacca cccgggcgcg cacggagatg gctgcgtgat cgccgtgtac
     1201 cccgaggcgg ccgccacggc ggaactcgtg ttcgagctgt gcgaggccct gggcgcgcct
     1261 ctgaccgtcg agctggccac ctgcctcctg acggggctca ccgacgatac cggagggttc
     1321 cggcacagca acaccacggc tcgcgctcac gagttggcct cgcggctggt cgcggccggg
     1381 gcggaccctg cccggatcgc cgagaacctg tttggcaacc agccgccggc ccgtctggta
     1441 ctgcggggcc gcgcgctggc ccgggccagg ctgctgctgg acggtcgagt gattctgtcc
     1501 tacctcacaa ataaagactt cgcggtcacc ggggccgatc gggccgatag ctcctacgtc
     1561 atcgacgacc tccggttggc agcggacatc caggtcgccg ccgtgctggt ggagacggag
     1621 aacggccagt gccgggtgag tctccggtcg tcctcggatc tggatgtggc ggcagtggcc
     1681 cggtcgctcg gcggcggggg gcacgtgaag gcggcggggt gcgagctacc cgggacagtg
     1741 gctcaggcgg agccgccgct cctggcggct ctggccggcg ctctgggggc cgacggccgg
     1801 catggtggat agagcgatgg tctccggcgt ggcacccatc tggaagccgc ggg
//
LOCUS       DAOSPL010001064         1851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_63089_length_1851_cov_2.727728, whole genome shotgun sequence.
ACCESSION   DAOSPL010001064 DAOSPL010000000
VERSION     DAOSPL010001064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63089_length_1851_cov_2.727728"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLQ54_16760"
     CDS             <1..297
                     /locus_tag="PLQ54_16760"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOS94971.1"
                     /translation="NEFDPDACRWNRDGVGRPCNTCPVTEYPRGVSPCGAMQMSGNVA
                     EYCADGPGPGAAFIKGGSWMNEDPINLRPAARNMSGFANNRSAFYGFRCAKEVV"
     gene            298..1056
                     /locus_tag="PLQ54_16765"
     CDS             298..1056
                     /locus_tag="PLQ54_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94972.1"
                     /translation="MARITLTPAPGQMSASATDVPAFPDERFGFGIPECIGDSARPVW
                     FGGVEPKWEERDGGAFETSGRVEGELEYSALLTPFEDHVDLNMKVTNLSDRDWAHAMA
                     FNCFQCGGALSIGDHECLRHWVGWRGELRRLIEVPRHCGPRPAVQLYGVDGAPPWEDI
                     PFVARFACSPPDVALEGWMAIVSRDGSRLVATVSRPCLCLFQNMEYSCIHASPWMGRL
                     GPGETGTALNRAYFVESSLEAWHARMVTEMAEEG"
     gene            complement(1148..>1851)
                     /locus_tag="PLQ54_16770"
     CDS             complement(1148..>1851)
                     /locus_tag="PLQ54_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94973.1"
                     /translation="MLPSLPPPPERKCVSMSSSGARLPFACRAVQFALAASFLVMVAG
                     ASAQGQADPAAPTECPAAYVVSFRMITVAPAEGRDPGEFGDVPSGAFFFAPDDGDGFY
                     DRVKLAEAYGRGVREGLVATVGPVYSMSMKPNSNPVVVSGVTGDGSGTWSVSASVEPL
                     TPDGKRVRLHSGWDVASLFGFEVPGLGTSDHWLGVPLPLHSGNRVGSPADGEAVGQKF
                     RGGYTILVLIEKAAP"
BASE COUNT          313 a          636 c          613 g          289 t
ORIGIN      
        1 aacgagttcg atcccgacgc ctgccgctgg aatcgcgacg gcgtgggacg tccgtgcaac
       61 acgtgccccg tgacggagta cccccggggc gtcagcccgt gcggagccat gcagatgagc
      121 ggcaacgtcg ccgagtactg cgccgacggg ccgggcccgg gcgcagcgtt catcaagggc
      181 ggctcctgga tgaacgaaga ccccatcaat ctccgcccgg ccgcgcggaa catgagcggc
      241 ttcgccaaca accggtcggc cttctacggg ttccgctgcg cgaaggaggt ggtctagatg
      301 gcccgcatca ctctgacgcc tgctcccggg cagatgtcgg cgtccgccac cgatgtcccg
      361 gctttccctg acgaacgatt cgggttcggc atccccgagt gcatcggcga ctccgcgcga
      421 cccgtctggt tcggcggcgt ggagcccaag tgggaggagc gcgacggcgg cgcctttgag
      481 acctcgggcc gcgtggaggg agagctcgaa tactccgcgc tactgacgcc cttcgaggac
      541 cacgtggacc tcaacatgaa ggtcacaaac ctcagcgatc gcgactgggc tcacgccatg
      601 gccttcaact gcttccagtg tggcggtgcg ctgtcgatcg gcgaccacga gtgtctgcgg
      661 cactgggtcg ggtggcgtgg cgagctccgg cggctcatcg aggtcccgcg gcactgtggc
      721 cccaggccgg ccgtgcagct ctatggcgtc gatggcgctc cgccgtggga ggacatcccc
      781 ttcgtcgcgc gattcgcatg ctcaccgccc gatgtggccc tcgaggggtg gatggccatc
      841 gtctcgcggg acggctcgcg cctcgtcgcc accgtcagcc ggccatgcct gtgcttgttc
      901 cagaacatgg agtactcgtg catccacgca agcccctgga tgggccgcct cgggcccggc
      961 gagaccggca ccgccctgaa ccgagcctac ttcgtggagt catcgctgga ggcctggcac
     1021 gcacggatgg tgactgagat ggccgaggaa gggtgagtgt tgcggcagac gagcccgaag
     1081 accgcacgcg gcgggccggg cagcactcgg ctggctcgac ccgccgcgcg tcctcccgac
     1141 cagctcgcta tggggccgcc ttctcgatca gcaccagaat ggtgtagccg ccgcggaact
     1201 tctgccccac cgcttcaccg tctgccgggg accccacgcg gttccccgag tgcagcggga
     1261 gcgggacgcc caaccagtgg tcgctcgtgc ccagacccgg cacctcgaag ccgaacaggc
     1321 tggcgacatc ccatcccgaa tggagccgga cccgcttccc atcgggcgta agcggttcga
     1381 cggacgcact gactgaccag gttccactgc cgtcgcccgt cacgccggag acgacgactg
     1441 ggttcgagtt gggcttcatg ctcatgctgt atacgggccc cactgtggcc acgaggccct
     1501 cgcgaacgcc ccgcccgtaa gcctcggcca gcttcacgcg gtcatagaag ccatcgccat
     1561 catcgggggc aaagaagaat gctcctgatg gtacatcgcc aaactcacca ggatctctcc
     1621 cttccgctgg ggcgaccgtg atcatgcgaa acgagactac ataggccgcg gggcactccg
     1681 tcggggcagc aggatcggcc tgcccctggg cactggcgcc ggccaccatg acgaggaatg
     1741 aagcagcaag ggcgaactga accgctctgc acgcgaatgg gagtctggca ccggacgacg
     1801 acatgctcac gcacttcctt tcgggtggtg ggggcagcga aggcaacatc a
//
LOCUS       DAOSPL010001065         1850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_63135_length_1850_cov_3.177159, whole genome shotgun sequence.
ACCESSION   DAOSPL010001065 DAOSPL010000000
VERSION     DAOSPL010001065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63135_length_1850_cov_3.177159"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PLQ54_16775"
     CDS             complement(<1..442)
                     /locus_tag="PLQ54_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94974.1"
                     /translation="MTILVADYGMNVYHGGMHDLAQRLESLKAIGYDGLERLTAATEA
                     QVLEAAATFRRLGCRFATVQAPTPDLCIRWSAAMGCTYVWASPTAPDLDGLVRQVNGQ
                     VRAAEAWGIRIGLHNHMGTPVESHEQVIGFLERCPEAGLVLDTAH"
     gene            complement(623..>1850)
                     /locus_tag="PLQ54_16780"
     CDS             complement(623..>1850)
                     /locus_tag="PLQ54_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94975.1"
                     /translation="RDLPPLEFTIESDGVVDSLEDPRVRLRCVEGRFVSQMVFGRPLV
                     HRVTMLIPTDPEALADPQRRGKVVIVGSIREFFHESFLCNYGYPIATRLRYPVMILPN
                     PGETPDQPDREWSITELWAEGVPRVLENHYFFRLAIPYLRAMDVFADTLGVPRDEIRA
                     VIGGHSKRAPSAYVAAAIRPDNVAGVVFMGMEGRWNPKPGNWGEYVSPVTLQHRVKAK
                     TIYLGATNEDGYTMFNVTRNQELLDVPWTVSMVPNYRHAAESPQQFLIWQMWVSHVFD
                     GRPVARISDVRHEETEKGIRFRARIDDPNRIVQVRAWYAYCDDVPLWRDIVWYPVLML
                     PSSADATRYEGYEHGKTPDAWLVEVEDIAQGVHGYVTSCPQNITGLDAQERVSRGSRS
                     RLWSPEKASAGPAPAP"
BASE COUNT          300 a          628 c          607 g          315 t
ORIGIN      
        1 ggtgggccgt gtcgagcacg agtccggcct ccgggcaccg ttcgaggaag ccgatgacct
       61 gctcgtggct ttcgacaggc gtccccatgt ggttgtgaag gccgatgcgg ataccccacg
      121 cctcagccgc ccgcacctgg ccgttcacct ggcggaccag gccatcgaga tccggcgcgg
      181 tgggactggc ccacacgtac gtgcagccca tggccgcgct ccagcggatg cacagatcgg
      241 gcgttggcgc ttggacggtg gcaaaccgac acccgagccg gcggaaggtg gcggcggcct
      301 cgagcacctg ggcctctgtg gccgcggtca gacgctccag gccgtcgtag ccaatggcct
      361 tgagcgactc cagccgctgg gccaggtcat gcatcccccc atggtacacg ttcatgccgt
      421 agtcggccac gaggatcgtc atgcgagcct ccctctccgt gcgtgcgcgc cgcgtgccgg
      481 ccaccgagat catgcccgcc cgggcaccgg cgaccatgca gtgtctcggg ccgcccggtc
      541 gccagaaagc gacccgagtg gaactgcgcg ccctttcgca gcccgcagac acaggtcctc
      601 gagtccttcg ggctgtccag tcctacgggg ctggtgccgg gccggcgctc gccttctccg
      661 ggctccatag gcgcgaccga gacccgcgcg acactctctc ttgcgcgtcg agtcccgtga
      721 tgttctgcgg acagctcgtc acgtagccgt gcactccctg ggcgatgtcc tcgacctcga
      781 ccaaccaggc gtccggcgtc ttgccgtgct cgtagccctc atagcgcgtg gcatccgcgc
      841 tcgaggggag catcagtacc ggataccaga cgatatcacg ccacagcggt acgtcgtcgc
      901 agtaggcgta ccatgcgcgg acctggacga ttcggttcgg gtcgtcgatg cgagcacgga
      961 accggatgcc cttctcggtc tcctcgtggc ggacgtcgct gatgcgcgcc accgggcggc
     1021 cgtcaaagac gtgagagacc cacatctgcc agatgagaaa ctgctgaggc gattccgcgg
     1081 cgtgccggta gtttggcacc atcgagaccg tccagggtac gtcgagcagc tcctggttcc
     1141 gggtgacgtt gaacatggtg tacccgtcct cgttggtggc gcccaggtag atggtcttcg
     1201 ccttcacccg atgctgcagt gtgacggggg aaacgtactc gccccagttg cccggtttcg
     1261 ggttccaccg gccctccata cccatgaaga cgacgcccgc cacgttgtcg ggccggatgg
     1321 ccgccgcgac gtaggcggag ggcgcgcgct tgctgtgccc gccaatcacc gcgcgtatct
     1381 catcgcgtgg aacgcctagc gtgtcggcga acacatccat ggcgcgaaga tacgggatag
     1441 ccagacgaaa gaagtagtga ttctcgagca cccgagggac tccctcggcc cagagctcgg
     1501 tgatgctcca ttcgcggtcc ggttgatccg gagtctctcc ggggttgggg aggatcatca
     1561 ccgggtagcg caggcgcgtg gcgatgggat agccgtagtt gcacaggaac gactcgtgga
     1621 agaactcgcg gatgctcccg acgatgacga ccttgcctcg ccgctggggg tccgccagcg
     1681 cctcgggatc ggtggggatc agcatcgtga cccggtgaac cagcggtctg ccgaagacca
     1741 tctggctcac gaaccgcccc tcgacacatc tcagcctcac ccgcgggtcc tcgagcgagt
     1801 caaccacacc gtcgctctcg atcgtgaact ccagcggcgg aaggtcccgg
//
LOCUS       DAOSPL010001066         1849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_63192_length_1849_cov_2.476589, whole genome shotgun sequence.
ACCESSION   DAOSPL010001066 DAOSPL010000000
VERSION     DAOSPL010001066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63192_length_1849_cov_2.476589"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /gene="lpxB"
                     /locus_tag="PLQ54_16785"
     CDS             <1..1272
                     /gene="lpxB"
                     /locus_tag="PLQ54_16785"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOS94976.1"
                     /translation="GVRGLSMSDFSGAAGPDLAPPPYRVLFSVGDPSGDVHAARVAAE
                     LKRRRSDIYLFGFGGPRMASAGVVLSHDNRGCSAIGVLESLRLVPRMHRILAALRQIL
                     HTTPPDLVVLVDYGVFNLRLAVTAKSVGVPVLYYIPPGCWARDMLRVTERVARWTDHV
                     ATPFPWSAGLLTRAGAHATFVGHPLLDGVESQPSREEARHRVGLGGDERGLAILPGSR
                     GHEIRHILPLMLRAAALARGQRPELVPLVSHAPGSDRVPLQRAASKLLPEARFVESTA
                     DLLRASDVGLVKSGTITLEAALAGLPMVVTYAASPVAALQWHLHAARRVKYIAIPNIL
                     ADREVVPERYGAQGRPPDLAREVLALYDDPARREAVRSDYAGLAAQLGQPGASRAVAD
                     LVEAMLDQAQRARTAGNARSRQASVADPWRR"
     gene            1275..>1849
                     /locus_tag="PLQ54_16790"
     CDS             1275..>1849
                     /locus_tag="PLQ54_16790"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOS94977.1"
                     /translation="MSRQMAAAQDFVATQGGSTRAADRLSRDALGRFYQYVRPYRHVL
                     VAAVISIGLAAGLQMTVVYLSKTILDVGLVTGRGVGESLHGMRSAALTILAVGLLLAA
                     AEFGQIFLSSTVALKAVRDLRTAMFHRLHTLPLAYYDRARSGELMSRLVNDTYVLQTA
                     LSVDVPSLVAAPVAVAAGLVGMFMLSWRVSAV"
BASE COUNT          245 a          610 c          657 g          337 t
ORIGIN      
        1 ggcgtccggg ggctcagtat gagtgacttc tcgggggcgg ctggacccga tctggctcct
       61 cccccctatc gcgtgctgtt ctcggtgggg gatccatcgg gcgacgtgca tgcggcgcgg
      121 gtcgcggcgg agctcaaacg gcgtcgcagc gacatctact tgtttggctt cggcggcccc
      181 cgcatggcca gcgccggagt cgtgctctcc catgacaacc gtgggtgctc ggccattggg
      241 gtgctcgaga gccttcgcct cgtaccgcgc atgcaccgca tcctcgccgc gctgcgccag
      301 atcctgcaca cgacaccccc agacttggtg gtgcttgtgg actacggcgt cttcaacctc
      361 cggctggccg tcacggccaa gtcggtggga gtcccggtgc tgtactacat cccgccgggt
      421 tgttgggcgc gagacatgct gcgggtgacg gagcgcgtgg cccgctggac cgaccacgta
      481 gccacgccat tcccgtggtc ggccgggctt ctgacccggg cgggcgccca tgcgactttc
      541 gtcgggcacc cgttgctcga cggtgtggag agccagccga gccgagagga ggcgcgccac
      601 cgagtcggtc tggggggcga tgagagaggg ttggcgatcc tccccgggag ccgggggcat
      661 gagatccggc acatcctgcc cttgatgctc cgcgcggccg cgctggcgag gggacagagg
      721 ccggagctcg ttccactggt gtcccacgct ccggggagtg atcgggtccc gctccagagg
      781 gcggcgtcga agctgttgcc ggaggcgcgc ttcgtcgaga gcaccgccga cctgctgcgt
      841 gcgtcggacg ttggcttggt gaaatccggg accatcacac tggaggcggc tttggccggg
      901 ctgcccatgg tggtcaccta tgcggcctca cccgtggcgg cgcttcagtg gcatctccat
      961 gcggcacgcc gggtgaagta cattgcgatc ccgaacattc tggccgaccg cgaggtggtg
     1021 ccggagcggt acggtgcgca agggcgcccc cccgaccttg cgcgcgaggt tctggccctc
     1081 tacgatgatc cggctcgacg cgaggccgtc cgatcggact atgccggcct cgccgcccag
     1141 ctcggacagc ccggcgcgag ccgagccgtg gccgatcttg tcgaggcgat gctcgatcag
     1201 gcccagcgag cgaggactgc gggcaacgcc cggtctcgcc aagcatccgt agcagatccc
     1261 tggagacggt agccatgtcc cggcagatgg ccgcggcgca ggacttcgtg gccacccaag
     1321 gcggttcgac tcgcgcggct gatcggctca gtcgggacgc gcttggccgg ttctatcagt
     1381 acgtacggcc gtaccgacac gttctcgtcg ctgcggtcat cagcatcggt cttgccgccg
     1441 ggctccagat gaccgtcgtg tacctgtcga agacgattct cgatgtgggg ctcgtcaccg
     1501 gccgtggcgt gggtgagtcc ctccacggga tgcggtcggc cgcgctcacg atcctcgcgg
     1561 tgggcctgct gttggccgcg gcggagttcg gccagatctt cctgtcaagc accgtcgccc
     1621 tgaaggcggt tcgcgatctg cggactgcca tgttccaccg cctacatacg ctcccactgg
     1681 cctactacga ccgggcgcgt tcgggcgagc tgatgtcgcg cctggtgaac gacacttacg
     1741 ttctgcagac ggcgctgtcg gtggatgtcc catcattggt ggccgcgccg gtagcggtgg
     1801 cggcggggct cgtcggcatg ttcatgctga gctggcgtgt ctcggcggt
//
LOCUS       DAOSPL010001067         1848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_63221_length_1848_cov_2.877858, whole genome shotgun sequence.
ACCESSION   DAOSPL010001067 DAOSPL010000000
VERSION     DAOSPL010001067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63221_length_1848_cov_2.877858"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PLQ54_16795"
     CDS             <1..941
                     /locus_tag="PLQ54_16795"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOS94978.1"
                     /translation="AMAAGTVLAARASAQATQEGPPMAADGPKVQWELMLPHEMKAAL
                     EACPTAFVPLGTLEWHGYQNALGLDALKAHALCVRAARQGGGIVLPPLYGGVGGVAEP
                     YTVVMEPEPTFSSHLLEPWLRGILAELKRIGFRAAIVLTGHYGASQQIAVRETAVRES
                     QRLAIPILGTPEYLLAMDAGYLGDHGGRFETSLLMALHPELVDLSRLEGPPPYRGIGG
                     GDPKRESSADEGERVSKVIVDRLARLARHMPRWDDTTRTAFIRAEQALVSHQLELGGR
                     AGDAWAAWHDARRYVPYGEWVAAERFAEIEAMARLP"
     gene            complement(983..>1848)
                     /locus_tag="PLQ54_16800"
     CDS             complement(983..>1848)
                     /locus_tag="PLQ54_16800"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOS94979.1"
                     /translation="SGVTSGLLPSVRIVEDGPVRTVVEAVMAYGQSAVCQRYKLPKQG
                     TEIEVEIRVFWAEKDRMLKLSIPVPPGDYRYVGQTAFAVTELPTNGDEAVAQQWTAAV
                     SRDGSRALTCINEGTYGSDFSEDGLRMTLLHSPAYSGHPIFDRPIVPQDRFSPRIDQG
                     ERVFRFWINGGPAEARLAAVDREAQVHQQCPFVLSFRPSGTGERPKPLVTLSDDAVVL
                     SAAKRAEGDDEALILRLYEPTGQGRETRLSLPWLGGELSVALSPFEVKTLRVNPDTGE
                     VTETDLLEQGV"
BASE COUNT          291 a          635 c          644 g          278 t
ORIGIN      
        1 aggcgatggc ggcgggcacc gtgctcgcgg cgcgcgcgag cgcccaagcc acccaggagg
       61 gaccaccgat ggcggccgac ggcccgaagg tccagtggga gctcatgctc ccccacgaga
      121 tgaaggctgc gctcgaggcc tgcccgacgg ccttcgtgcc tcttgggaca ctcgagtggc
      181 acggctacca gaacgcgctg ggcctcgatg ccctcaaagc ccacgccctg tgcgtgcgcg
      241 ccgcgagaca gggcggcggg atcgtgctac caccgctcta tggcggcgtg ggtggggtcg
      301 ccgagcccta cacggtggtg atggagccgg agccgacgtt ctcgtcccac cttctcgagc
      361 cgtggcttcg aggcatcctc gccgagctca agcgcattgg tttccgggcc gctatcgtgc
      421 tgactggcca ctacggagcg tcccagcaga tcgcggtccg cgagacggcc gtccgcgaga
      481 gccagcgcct ggcgattccg atcctcggca cgcccgagta cctcctggcc atggacgccg
      541 gctatctcgg cgatcatggt gggcggttcg agacatcgct actgatggca ctccatcctg
      601 agctcgtgga cctgtcccgc ctcgagggcc cgccgcccta caggggcata ggcggcggcg
      661 acccgaagcg tgagagctca gctgacgagg gggagcgggt ctcgaaggtg atcgtcgacc
      721 gcctggcccg gctggcgagg cacatgccac ggtgggatga cacgacgcgc acggccttca
      781 tccgagcgga acaggcgctg gttagccacc agctcgagct gggcggtcgc gccggcgatg
      841 cgtgggcggc ctggcacgac gcgcgccgct acgtccccta cggcgagtgg gtcgccgccg
      901 agcggtttgc cgagatcgag gcgatggcac ggctgcctta ggcgccgttc agggccggcg
      961 cggcaccggg ccgctggccg tctcacactc cctgttccag cagatcggtc tcggtaacct
     1021 cgcccgtgtc ggggttcacg cggagggtct tcacctcgaa gggtgaaagc gcgaccgaga
     1081 gctcgccgcc cagccagggc aacgacaggc gcgtctcgcg cccctggccg gtgggctcat
     1141 agagccgcaa gatgagcgcc tcatcgtcgc cctcagcccg cttggcggca gacagcacga
     1201 cggcgtcgtc gctgagtgtg accagcggct tcggccgctc ccccgtaccg ctggggcgga
     1261 aagaaagcac gaacgggcac tgctgatgga cctgggcctc gcgatccacg gcggccagtc
     1321 gcgcctcggc gggtcctccg ttgatccaga accggaacac ccgttccccc tggtcgatgc
     1381 gcggcgagaa ccggtcctgc ggaacgatgg gccggtcaaa gatgggatgt cccgaatagg
     1441 cgggcgagtg gagcagcgtc atccgaaggc cgtcctccga gaagtcggag ccgtaggtcc
     1501 cctcattgat gcaggtcaac gctctcgagc cgtccctcga gacggccgcc gtccactgct
     1561 gggccacggc ttcgtcaccg ttggtgggca gctcggtgac ggcgaaggcg gtctggccca
     1621 cgtagcgata gtcgcccgga ggcaccggga tactgagctt gagcatgcgg tccttctccg
     1681 cccagaagac gcggatctcc acctcaatct cggtgccctg cttcggcagc ttgtagcgct
     1741 ggcagacggc cgactgtccg taggccatca cggcctcaac caccgtgcgc acggggccat
     1801 cctcgacgat gcggaccgag ggcagcaggc ccgaagtcac tccggaga
//
LOCUS       DAOSPL010001068         1846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_63325_length_1846_cov_2.906756, whole genome shotgun sequence.
ACCESSION   DAOSPL010001068 DAOSPL010000000
VERSION     DAOSPL010001068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63325_length_1846_cov_2.906756"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..881
                     /locus_tag="PLQ54_16805"
     CDS             87..881
                     /locus_tag="PLQ54_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS94980.1"
                     /translation="MRGRRGFTLIELLVVIAIIAILAAILFPVFAKAREKAEETQCVA
                     NVSQIVKAILMYASNWDNRLPDSNPCFYDNSNPHRSAPNFSWAGAIQHEVNNWQVFLC
                     PAHPEFEDSWVDSGDPADPYPGHQAKLGYSYNGSYWLGCPGTGQPRDAYSGLDGYITL
                     TRCQDPANTIMVTDSGTQGRSECGDAMPGNTQPWSQDKAAHAQAWADGVDIGVQYYPE
                     MRHNLGFIVGFIDGHAKWTPFEKPIVEWWMWTIEKDGSTSASPSGH"
     gene            969..1775
                     /locus_tag="PLQ54_16810"
     CDS             969..1775
                     /locus_tag="PLQ54_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOS94981.1"
                     /translation="MARERLDTVVVHRGLLPTREKARAAIMAGMVTVDGQVEDKPGHG
                     VRPDSVVAVARAPHPYVSRGGVKLEAALRSFAVDVSGCVAADLGASTGGFTDCLLQHG
                     AARVYAVDVGRGQLDARLVSDPRVVVLDDTNARALTPGDLPELVDMVTMDLSFISLTK
                     VMPAVRWLLSPTARAVIALVKPQFEAGRDAVGRKGVVRDPAVWRSCIERVGECARPLG
                     LHAEGVVPSPIRGPKGNTEFLLLLRPISSTGDDDTFDEALDAAIEAAQLL"
BASE COUNT          318 a          587 c          635 g          306 t
ORIGIN      
        1 tgtgtggtga gttttgcgcc gagccgtgaa actgcaggcc ggtggaagca tcaaaaggag
       61 cgcaaggacc cgaaggaggt tacaccatgc ggggacgccg tggctttacg ctcatcgagc
      121 tgttggtggt catcgcgatc attgccatcc tcgcggccat tctgttcccc gtgttcgcca
      181 aggctcggga gaaggccgaa gagacgcagt gcgtggcaaa cgtgagccag atcgtcaagg
      241 cgatcttgat gtacgcctcg aactgggaca accggctgcc ggactcgaac ccgtgcttct
      301 acgacaactc caatccgcac cgatcggccc cgaacttctc gtgggccggc gcgatccaac
      361 acgaggtcaa caactggcag gtgttcctgt gtccggccca ccccgagttc gaggactcct
      421 gggtggactc gggcgacccg gccgacccgt acccggggca ccaagccaag ctgggctact
      481 cgtacaacgg gtcgtactgg ctcgggtgtc cggggaccgg acagccgagg gatgcctact
      541 ccggtctcga tgggtacatc acactgactc gatgccagga tcccgcgaac accatcatgg
      601 tgaccgactc gggcacgcag gggcggagcg agtgtggcga cgccatgccg ggtaacacgc
      661 agccgtggag ccaggacaag gccgcccacg cgcaggcctg ggccgatggc gtcgatattg
      721 gcgtgcagta ctacccggaa atgaggcaca acctgggctt catcgttggc ttcatcgatg
      781 ggcatgccaa gtggacgcct ttcgagaaac ccatcgttga atggtggatg tggacgatcg
      841 agaaggatgg ctccaccagc gccagcccga gcggacactg acgtgggtgg ggagcacggc
      901 gccagccggg gcccggccgt actgggggcg gccgggcccc tcggcatcgt agtggacggg
      961 gcgcaccgat ggcgagagag cgactggaca cggtggtggt gcaccggggc cttctgccca
     1021 cgcgggagaa ggcccgcgcc gccatcatgg ccggaatggt gacggtggac ggccaggtcg
     1081 aggacaagcc gggccacggc gtccggccgg actcggtggt ggccgtcgcc cgcgcgccgc
     1141 acccctatgt gtcacgggga ggcgtgaaac tggaggccgc ccttcggtcg ttcgctgtgg
     1201 acgtcagcgg ttgcgtggca gccgatctgg gcgcatccac gggagggttc accgactgcc
     1261 tgctccaaca tggtgcggct cgcgtatacg cggttgacgt gggccgcggc caactcgatg
     1321 cccgattggt ctccgatccg cgtgtggtcg tcctcgatga caccaacgcc cgcgccctga
     1381 cccccggcga cctgccggag ctcgtcgaca tggtcaccat ggacctctcg ttcatctcgc
     1441 tgaccaaggt gatgcccgcc gtgcgatggc tgctgagccc cacggcccgg gcggtcatcg
     1501 ccctggtcaa gccgcagttc gaggcaggcc gagacgccgt ggggcggaag ggagtggtgc
     1561 gcgatcccgc tgtgtggcgg agctgcatcg agcgcgtggg cgagtgcgcg cgcccgctgg
     1621 gcttgcacgc cgaaggcgtt gtcccctctc ccatccgcgg ccccaaaggc aacaccgagt
     1681 tcctcctgct gctccgcccc ataagctcga cgggcgacga cgacactttc gacgaggcgc
     1741 tcgatgccgc catcgaggcg gcccagctcc tctaatgacc tttgcgtcct tggattgaag
     1801 gtacggaatg ggtttgtctg gtacatgtgt gcatggccaa accagc
//
LOCUS       DAOSPL010001069         1845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_63378_length_1845_cov_3.167598, whole genome shotgun sequence.
ACCESSION   DAOSPL010001069 DAOSPL010000000
VERSION     DAOSPL010001069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63378_length_1845_cov_3.167598"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..189
                     /locus_tag="PLQ54_16815"
                     /pseudo
     CDS             1..189
                     /locus_tag="PLQ54_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370968.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
     gene            196..753
                     /locus_tag="PLQ54_16820"
                     /pseudo
     CDS             196..753
                     /locus_tag="PLQ54_16820"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
     gene            807..>1845
                     /locus_tag="PLQ54_16825"
     CDS             807..>1845
                     /locus_tag="PLQ54_16825"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOS94982.1"
                     /translation="MSTTPRRAALPALAGILVLLAGRPASASWLSENLERLYGSYVKV
                     MLEAQVPLEKDALLNDMVRDMFGELVEAGGRPNLEHRIRILDTDMVNAMAAPGGYVWV
                     TKGMLRFVDTPDELAAVLGHELGHVTYRHGWENLKRDFAVDMLLNALQATQFADFTGW
                     ASVLYYFKALHYSREDELEADRAGATFSQLAGWDNLRMSEFFTRLAKEDGHQSKFDIF
                     FSTHPSHEQRLEQLSTLPEFAITDPAVLVRIGDNYAARYLFNQAAESYQAALKLDPDH
                     ADAHARLASVYGFLGMDDRARESLARARSLSGGALPVATRDELVAGIVDALAAASQNR
                     APMPAAGGLDVS"
BASE COUNT          304 a          657 c          599 g          285 t
ORIGIN      
        1 gtcgagaaca tggcgggcta tgcctgcccc cattgcggcg ggatttccga tcctttcgga
       61 tcgggtggcg ccgaggcggc ggcaaaatcg atggaactcc ccttcctcgg acgcattcct
      121 ctcgatatcg cgatccgcaa ggcatcggat gcaggccagc ccccggcggc ggccgacgat
      181 gacgaggcct acgagaccct caggcagcac tcggccgagg ccgcagaggc cacccgcgcg
      241 ctggcggcgc gctacggcta tcgcgagccc ggcgaggccc acgtcgccgg cctcctgcac
      301 gatgtcggca aactcgtgat ccgcctccag ttccccgagg aatacgcggc gattgtggcc
      361 caccaacagt catcgcgact gccggccgtc gtcgccgagc ggaagtacct gggagccacc
      421 catactgaga tcggcgggtg ggtcgctgag atgtggcagc tccccgccac gctccgtgac
      481 gcgctactct atcaccacga gccgggagcg gctcgggaga actcgctgct ggtggcgctc
      541 gttcatctgg gggatgtcgt cgcacatacc gcggccggca tggcggggcg cgcgtctgcc
      601 tccccgccca tcagtgcgtc aggctgggcc gttctgtcgg cccgccgttc cgacatccaa
      661 caggaggaga tccgtgcctt cgccgaagaa ctgatcggtg cctgggatcg ggtccaggcc
      721 ctggcatacg ggctgagcgc ggcggcaggc tagtgccgcc gcggtctcgg cgcgggcgcg
      781 gcaaggcgcc acagggagcg atccgcatgt ccaccacgcc acggcgagcg gccctacctg
      841 ccctcgccgg cattctcgtg ctcctggccg gccgcccggc cagcgcgtcc tggttgtcgg
      901 agaacctcga gcgcctctac ggatcctacg tcaaggtcat gctcgaggca caggtgcccc
      961 tcgaaaagga cgccctcctc aacgacatgg tccgcgacat gtttggcgag ttggtcgagg
     1021 caggcggacg cccgaacctc gagcaccgca tccgcatcct cgacaccgac atggtgaacg
     1081 ccatggccgc tcccggcgga tacgtctggg tcaccaaggg catgctgcgc ttcgtcgaca
     1141 ccccggacga gctggctgcc gtgctcgggc acgagctggg acacgtgact taccgccacg
     1201 ggtgggagaa tctgaagcgt gacttcgccg tggacatgct cctgaacgcg ctgcaggcca
     1261 cccagttcgc ggacttcacc ggatgggcca gcgtgctcta ctacttcaaa gcccttcact
     1321 atagtcgcga ggacgagctc gaggccgatc gtgcgggagc caccttctcc cagctcgccg
     1381 ggtgggacaa cctccgcatg tcggagttct tcacgcggct ggccaaggag gatgggcacc
     1441 agtcgaagtt cgacatcttc ttctcgaccc atcccagcca cgagcagcgc ctcgaacagc
     1501 tcagcacgct acccgagttc gccatcaccg acccggcagt actggtgcgg atcggcgaca
     1561 actacgcggc gcggtacctc ttcaaccagg ccgcggaaag ctaccaggcg gcgctcaagc
     1621 ttgaccccga ccacgcagac gcccacgccc gcctcgccag cgtctacggg ttcctcggaa
     1681 tggacgaccg tgccagggag tcgctggctc gcgcccgcag cctgtcgggc ggagccctcc
     1741 cggtggccac gcgggacgaa ctcgtcgccg gcattgtcga cgcgctggcc gccgccagtc
     1801 agaaccgggc gcccatgccc gcagccggag ggctcgacgt gagtg
//
LOCUS       DAOSPL010001070         1845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_63395_length_1845_cov_2.493855, whole genome shotgun sequence.
ACCESSION   DAOSPL010001070 DAOSPL010000000
VERSION     DAOSPL010001070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63395_length_1845_cov_2.493855"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PLQ54_16830"
     CDS             <1..873
                     /locus_tag="PLQ54_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOS94983.1"
                     /translation="IAIRQVLLAADKPVIVTNATGCLEVATTIFPYTAWAVPFIHSAF
                     ENSAATASGLEAAYRSLRRQGKYSDEVYFLALGGDGGTYDIGLQALSGALERGHNFVY
                     VCYDNQAYMNTGIQRSSATPFGADTTTTPCGSARPGKTQRRKDLTQICAAHGIPYAAQ
                     TTIANWRDVVKCAEKAFKAEGPALLNILAPCPRGWRYESDETVNMARLSRDTCVWPCY
                     EIENGIVSISYKPKEKKPVKDWFAAQARFRHMLRPDNAALLEEAQAEVDMKWAQLQTL
                     EAVAKAGTEAAAAE"
     gene            964..>1845
                     /locus_tag="PLQ54_16835"
     CDS             964..>1845
                     /locus_tag="PLQ54_16835"
                     /inference="COORDINATES: protein motif:HMM:NF024941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HOS94984.1"
                     /translation="MRERGLAHSRGRSLGNGAWPLLLLCCTTASSGAPRVALVPLDSR
                     PPCHSMAILVGQAGGAQVVTPPREWLGTLERPGDCERILEWLSAQAPRCQAAVVSLDM
                     VTDGGLVASRTPATPAEVALGRVRQLADIRRLLPPGAPLFVFDTIQRIAPTTLDPESG
                     DLAARIAEYVHCASEAEATGDADTVARAIRLRERVPAGALEDYYRARQRNHQINLALL
                     EQLRQGIVDFAVLGLDDTAVYGPHVPEVARLRAVAAEWRLWDRLGLYPAADEAAMVLI
                     ARATQAVRGTRLRVDVRI"
BASE COUNT          303 a          651 c          608 g          283 t
ORIGIN      
        1 atcgcgatcc ggcaggtcct gctcgcggcc gacaagcctg tgatcgtgac caacgccacc
       61 gggtgtcttg aggtcgccac cacgatcttc ccatacaccg catgggccgt gcccttcatc
      121 cacagcgcct tcgagaactc ggcggctacg gccagcgggc ttgaggcggc ctatcggtca
      181 ctccggcgcc agggcaagta cagcgatgaa gtgtacttcc tggccctggg cggcgatggg
      241 ggaacctacg acatcggtct ccaggccctg tccggtgccc tcgagcgcgg ccacaacttc
      301 gtctatgtgt gctacgacaa ccaggcctac atgaacacag ggatccagcg gtccagtgcc
      361 acgccctttg gcgccgatac caccacgacc ccgtgcggct cggcccggcc ggggaagacg
      421 cagcggcgca aggacctcac ccagatctgc gcggcgcacg gcatccccta cgccgcacaa
      481 acgaccatcg ccaactggcg cgacgtggtc aagtgcgccg aaaaggcgtt caaagccgaa
      541 gggccagcct tgctgaacat cctggccccg tgccctcggg gttggcgata tgaaagtgac
      601 gagaccgtca atatggccag gctctcgcgc gacacctgtg tgtggccgtg ctatgagatt
      661 gagaacggca tagtgtccat ctcctacaaa cccaaggaga agaagcctgt gaaggactgg
      721 ttcgctgccc aggcccggtt ccgtcacatg ctgcgcccgg acaatgccgc cctgctcgag
      781 gaagctcagg ccgaggtcga catgaagtgg gcccagctcc agaccctcga ggctgtggcg
      841 aaggccggca ccgaggcggc agcggcggag tagcggcccg agcactggac gcccagtaag
      901 aacgagagcc ggtctcaggc gagaccggct ctctggcggt tgctcagacg gaggtcgggt
      961 ctcatgcggg aacggggcct agcccattct cgggggcgct cgctcggaaa cggggcctgg
     1021 ccccttcttc tgctctgctg caccaccgct tcttctggag ctccgcgcgt cgcgctggtc
     1081 cccctcgact cgcgcccgcc ttgccactcg atggccatac tcgtgggcca ggccggcggc
     1141 gcccaagtcg tcacgccccc gcgcgagtgg ctgggcacac tcgagcgtcc gggcgactgc
     1201 gaacgtatcc tcgagtggct ctcggcccag gcgccacgat gccaggcagc cgtcgtctcg
     1261 ctcgacatgg tcaccgacgg cgggctcgtg gcatcacgga cgccggcgac gccggccgag
     1321 gtggcgctcg ggcgcgtccg gcagctcgcg gacatccgac ggctcctgcc gcccggcgcg
     1381 ccgctgttcg tctttgacac aatccagcga atcgcgccga ccacactcga ccccgaaagc
     1441 ggcgacctgg cggcgcgtat cgccgagtat gttcactgcg cctcggaggc agaagccaca
     1501 ggggatgccg ataccgtcgc tcgtgccatc cggctccgcg agcgggtacc cgcgggcgcc
     1561 ctcgaggact actaccgggc gcggcagagg aaccaccaga tcaacctggc cctcctggag
     1621 cagctccgcc agggcatcgt ggatttcgcg gtgctcggcc tggatgacac ggccgtctac
     1681 ggaccgcacg tgcccgaggt cgcgcggctg cgggccgtgg cggccgaatg gcgcctttgg
     1741 gaccgcctgg gcctctatcc ggcggccgac gaggcggcga tggtcctcat cgcccgtgcc
     1801 acgcaggccg tccggggcac tcggctgcgc gtcgatgtcc ggatc
//
LOCUS       DAOSPL010001071         1844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_63430_length_1844_cov_2.943544, whole genome shotgun sequence.
ACCESSION   DAOSPL010001071 DAOSPL010000000
VERSION     DAOSPL010001071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63430_length_1844_cov_2.943544"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="PLQ54_16840"
     CDS             <1..1181
                     /locus_tag="PLQ54_16840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94985.1"
                     /translation="AAPLPQPSMAGWFSVEDFGRWCQEREQYAVIGDPTSALRDLRRL
                     DAYAAFLTNRSGVVLRDLRQAALLKLRVQAARGIILAHAGRAQEIKDGAVVERAAAWL
                     AAADEANEALSSLTVDLAQDRALFLTVVPVRTLAVAMCLATAIWLCLQGAGVMDHQGH
                     GGAAAPFRLGPVVLMAFISVLPVAFLIGAALLSPRLATAGLSPDLLAVANTFGGALLA
                     PDRDLAVGLSSVPVEVRSPALGAGVAAAPAVLVYLGACAHTRRLFWRRGTRHGLLGVL
                     LSIVPPPVAVVHWRTVATASAIAVILLSAGSSAAVHVHEARYSEPPEICFDTACFARV
                     LLDTEAHAVGEATAGTGASDAVRLDDARDHALALIEQYRRADSEALRGAGYVQEPPSR
                     "
     gene            1252..>1844
                     /locus_tag="PLQ54_16845"
     CDS             1252..>1844
                     /locus_tag="PLQ54_16845"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS94986.1"
                     /translation="MTKGISQIVFAKDRPLREVLRFSVDAGYQAVELCMRPKGELTPE
                     STPDDYRRLTALAREFGVTYTSVLPLCGGALTSNDPEERAKAVAVFGKCMEAAEALEA
                     GHVLCVPGYVGPDIRDDVAHQRAREGLLQLAPEAERRQVRLCIENVWNKMFMSPTELR
                     WFVTDLGSPWVNVYLDVGNMLIWGFPEHWIGVLGQQIR"
BASE COUNT          253 a          591 c          654 g          346 t
ORIGIN      
        1 gtgcggcgcc gctgccgcag ccttcgatgg ccggctggtt ctcagttgag gactttggac
       61 gctggtgtca ggaacgcgag cagtacgccg tgatcgggga tccgacctcg gcacttcgcg
      121 acctgcggcg gctggacgcg tatgcggcat tcctcaccaa tcggtctggc gttgtcctgc
      181 gtgaccttcg gcaggcggcg ctcctgaagc tgcgcgtaca ggcggctcgg gggatcattc
      241 tggcccacgc gggccgtgcc caagagatca aggacggtgc cgtcgtggag cgcgccgcgg
      301 cctggctcgc cgctgccgac gaggccaacg aggcgttgtc cagcctgacg gtcgacctgg
      361 cccaggatcg ggcgctgttt ctcaccgtgg taccagttcg gaccctcgcg gtggccatgt
      421 gtctggccac cgcgatctgg ctctgtcttc agggggcggg ggtcatggac caccagggcc
      481 acgggggagc cgcggcaccg ttccggctcg gccccgtggt cctcatggcg ttcatatcgg
      541 tgcttccggt ggcgtttctc atcggggccg cactgctctc cccccgactg gcgaccgcgg
      601 ggttgagccc tgatctgctc gctgtggcga acaccttcgg tggcgcgctc ctggcgccgg
      661 atcgggacct ggccgtgggg ctgtcgtcgg ttccagtcga ggtccggtct ccggctctcg
      721 gcgccggtgt agctgcggca cctgccgtgt tggtctacct gggcgcctgc gcccacaccc
      781 ggcgtctatt ctggcgccgc ggcacccgcc acgggctctt gggggtcctt ctgagtatcg
      841 tcccgccgcc ggtcgccgta gtccactggc gcactgtggc cacggcgtct gccatcgcag
      901 tgatactgct gtccgcgggc tcatcggcgg cggtccacgt ccacgaggcc cggtacagcg
      961 agccccctga gatctgcttc gacacagcct gcttcgctcg ggtcctcctc gacacggagg
     1021 cccatgcagt tggggaggcg acggcgggca cgggtgcctc ggacgcggtc aggcttgacg
     1081 atgcgcgtga ccatgctctt gcgttgatcg agcagtaccg cagggccgac tccgaagccc
     1141 ttcgcggggc ggggtacgtg caggagccgc cgtctcgctg aggaagtcgt cgggcgagtc
     1201 tccgccgcgc aggcaacccg atctggtgcc aagcgaaagg aaggttacta cttgaccaaa
     1261 gggatctctc agattgtatt cgcgaaggac cggccgctgc gcgaggtgtt gcgcttctcc
     1321 gtcgacgccg ggtatcaggc cgtggagctc tgcatgaggc ccaagggcga gctaacgccg
     1381 gagtcgaccc ccgatgatta ccggcggttg accgcgctgg cccgagagtt cggagtgacc
     1441 tacacgagcg tcttgcccct gtgcggtggc gcgctgacgt cgaatgaccc cgaggaacgc
     1501 gcaaaggccg ttgccgtgtt cggcaagtgc atggaggcgg ccgaggccct ggaagccggc
     1561 cacgtgctgt gcgtgcccgg gtacgtcggg ccagacatcc gcgatgatgt ggctcaccag
     1621 cgggccaggg aggggctgct gcagttggct ccagaggcag agcggcgtca ggtccgcctg
     1681 tgcattgaga atgtctggaa caagatgttc atgtccccta cggagctgcg ttggttcgtg
     1741 accgatctgg gatccccctg ggtcaacgtg tacctcgatg tgggcaacat gctgatctgg
     1801 ggtttccccg agcactggat tggagtgctg ggccagcaga tccg
//
LOCUS       DAOSPL010001072         1844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_63453_length_1844_cov_1.863611, whole genome shotgun sequence.
ACCESSION   DAOSPL010001072 DAOSPL010000000
VERSION     DAOSPL010001072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63453_length_1844_cov_1.863611"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PLQ54_16850"
     CDS             <1..476
                     /locus_tag="PLQ54_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94987.1"
                     /translation="AGLALSLLIALYQGWLVAYLGVPSFVVTLGGLMSFRGVAFLISD
                     GRTQPVTDADFLLIGGGLVLGISAIRSCALRVALHPAGFLLAATYPGDRIWFSMLIAW
                     ALKSILQRLGGYRAMRTARPYVYGMVVGDALAALVWIVVGWWLRNPDQRYFILPP"
     gene            594..1256
                     /locus_tag="PLQ54_16855"
     CDS             594..1256
                     /locus_tag="PLQ54_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOS94988.1"
                     /translation="MAQRTHHGFTLLELLVAVTILAVLAAILLPVFARAREKARQAHC
                     QSNLRQMTTAVLMYASDYDDQTPSANFRADLILIQPYLKNQDVFRCPSDSGYYEVCGD
                     HITGRHLPSIRVPNGYVANACFFGGAWFPSPNPYRSRRLDEFDQPTDIPIYADHHGNQ
                     GIVLPSVWPTHVSPAHPDDGGTNSSCRIRRRHTGTCSFGFGDGHVKPLPKAPEQVKWV
                     GE"
     gene            1334..>1844
                     /locus_tag="PLQ54_16860"
     CDS             1334..>1844
                     /locus_tag="PLQ54_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94989.1"
                     /translation="MGIQALRAVGAWLAMGLALAPAAPAQTGESALAPAPPTSAHATL
                     SGMCLVASPGEVLAVAPRAPLLSVSDREALSDWTPRTRPAVRAPSDGAPERDTGFHLG
                     KPHPERALGAILATGMLVAGDPGIIAAMAGSVPRQTRHSPPVQAFTRLVDLLPAAVAG
                     ATLLGHGDLH"
BASE COUNT          250 a          620 c          634 g          340 t
ORIGIN      
        1 tggccggcct ggcgctgtcg ctgctgatcg ccctttacca gggctggctc gtcgcctacc
       61 tgggggtgcc ttcgttcgtg gtcacgctgg gcgggctgat gtccttccgc ggcgtcgcct
      121 tcctgatctc cgacggccgc acgcagccgg tgaccgacgc cgacttcctg ctgatcggcg
      181 gcgggttggt gctgggcatc tcggccatcc ggtcctgcgc cctacgggtg gccctgcatc
      241 cggccggctt cctactggcg gccacgtacc cgggggaccg gatctggttc tcgatgctga
      301 ttgcgtgggc tctcaagtcg atacttcagc ggctgggcgg gtaccgtgcc atgcgcacag
      361 cgcggccgta tgtgtatggt atggtggtcg gcgacgcgct ggcggccctg gtgtggatcg
      421 tcgtcggctg gtggctgaga aaccccgacc aacgttactt cattctcccg ccgtagccgt
      481 ctcggcgccc gggtggggct gatgggccgg cgtatcgtgg cgaaggttgg ctggggcggg
      541 gcgccgaagt gtctcagtga ctacaacgat aggagtgccg cagagggcct ggtatggcgc
      601 agcggacgca ccatggcttc accctcctcg agttgctggt cgcggtcacg atccttgccg
      661 tgctggccgc cattctgctt cccgtgttcg cccgagcgcg agagaaggcc cggcaggcgc
      721 actgccagag caacctgagg cagatgacca cggcggtgct gatgtacgcg tcggactacg
      781 acgatcagac gcccagcgcc aatttccgcg ccgacctgat tctgatccag ccgtacctca
      841 agaaccagga cgtgttccgc tgcccctcgg attccggcta ctacgaggta tgcggcgatc
      901 acatcacggg tcgtcatctg ccgtcgatcc gggtcccgaa cgggtatgtg gccaatgcat
      961 gcttctttgg cggtgcgtgg ttcccatcgc ccaacccgta tcgcagtcgc cggttggacg
     1021 agttcgacca gccgaccgac atcccgatct atgccgacca ccacggcaac caggggatcg
     1081 tgctgccctc agtgtggccg acccacgtca gcccggcaca tcctgacgat ggtggcacca
     1141 acagctcctg ccggatccgg cgccgccaca cgggcacgtg cagcttcggc tttggcgacg
     1201 gacatgtgaa gccgctaccg aaggcacccg agcaggtgaa gtgggtgggg gagtaggctt
     1261 tcggagggtt cgccgcccga gcgttcgaat ctaggtaggc tctatcgacc gccctcccac
     1321 aggtgaacgc ctcatgggca tccaagccct ccgggctgtg ggggcctggc ttgccatggg
     1381 gctggctctg gctcccgcgg cacctgcaca gacaggcgaa tcggcgttgg ccccggcccc
     1441 gccgacttcc gctcatgcca cgctctcggg gatgtgcctc gttgcgagcc cgggtgaggt
     1501 gctcgcggtg gcgcctcgtg ccccgctgct ctcggtgtcg gaccgcgagg cgctttcgga
     1561 ctggacaccg cgaacgcggc cggccgtccg cgccccgagc gacggggcgc cggagcggga
     1621 cacggggttc cacctgggga agccgcatcc ggagcgtgct cttggggcga tcctggccac
     1681 cggaatgctg gtggccggcg acccgggcat catcgccgcg atggcggggt cggttccgcg
     1741 acagacgcgg cactcgccgc cggttcaggc cttcactcgt ctggttgacc tcttgcccgc
     1801 cgccgtggcg ggcgctacgc tcttgggaca cggcgacctg cacg
//
LOCUS       DAOSPL010001073         1843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_63491_length_1843_cov_2.595078, whole genome shotgun sequence.
ACCESSION   DAOSPL010001073 DAOSPL010000000
VERSION     DAOSPL010001073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63491_length_1843_cov_2.595078"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1832
                     /locus_tag="PLQ54_16865"
     CDS             <1..1832
                     /locus_tag="PLQ54_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94990.1"
                     /translation="VLRERHRRDGLRGLLLVHVERLRKLVVQAEQEDGRCVREVRPSS
                     KAQAELERYFLGRLSQMAAQTKLSLIETLVTGSPGIGGQPFLDRLGRRLANFREFMTG
                     FLKGHDERAASITGSAYQRLTRDLGIQVADGDIAELADRCLGHKEGLSREDELRYRES
                     LFANLARAHLGAGSNDPQPSLRHQLFIQDLAREGESVFDPSVYQGSLLDLPRYFSGFE
                     QGVRILFVGHHEPPIAEDLRGRIPMAQLVERLNVYNVSALDKFEAQARRLQRDWQQYL
                     RDEIMRSTPMLPLDLNRESYRCVGRRPRRAGFWGICPNPDRLDQIRDLMRQVMSDPAF
                     SGIATACDQQYFPVVSPTSEFDRYRMSFILVHGAVTTDIIYGYSVGDGERRKKVQVEP
                     SPYAHVLPERLPPRDLMRRAEEAYLLGRILGILRGEGARNEGVFPGTQTRPPTRVDLH
                     RDLMIGSVDLIRRAEGRGLECLEEAVLARIEELGPDSDALAEVLFRSFGQWEGVDSPP
                     EELIRNLGIVRVRAALDAEVPGEDDADLEIQAVRHVVLRFLERRNLLRDRIEAMSRPA
                     ISNVREEYIDGATQWVCTTCGAPYHRRRPLYGDVCENCNTMFH"
BASE COUNT          293 a          625 c          607 g          318 t
ORIGIN      
        1 cggtcctgcg cgagcggcat cgacgggacg ggcttcgggg tctgctcctt gttcacgtag
       61 agcgtcttcg gaagctggtc gttcaggccg agcaggaaga cggccgatgc gtgcgagagg
      121 tacgcccctc ctcgaaggct caggccgagc tcgaacgcta cttcctgggc cggctgagcc
      181 agatggccgc gcagacgaag ctcagcctga tcgagacgct ggtcacgggc agcccgggga
      241 ttggtgggca gccgtttctg gatcgactcg gacggaggct cgccaacttc cgcgagttca
      301 tgactggctt cctcaaggga cacgacgagc gtgccgcatc catcaccggc tcggcctacc
      361 agcgcctcac ccgcgacctc ggcatccagg tcgccgatgg ggacatcgcc gagctcgccg
      421 accggtgcct cgggcacaag gaggggctgt cccgcgagga tgagcttcgc taccgcgagt
      481 cgctctttgc gaacctggcc cgcgcccacc tgggagccgg ctccaatgat cctcagccgt
      541 cactgcgcca tcagctcttc atccaggacc tcgcacgaga gggcgagagc gtatttgacc
      601 cctcggtgta ccagggcagc ctgctggatc tgccgcggta tttcagcggt ttcgagcaag
      661 gcgtgcggat tctgttcgtg ggtcaccatg agccccccat cgccgaggac cttcgcggcc
      721 gcattcccat ggcacagctg gtcgagaggc tgaacgtcta caacgtgagc gcgctcgaca
      781 agttcgaggc gcaggcccgg cggctgcagc gtgactggca gcagtacctg cgtgacgaga
      841 tcatgcggag cacgcccatg ctgccgctgg acctcaaccg cgagtcgtac cggtgcgtcg
      901 gccgccggcc tcgtcgggcc gggttctggg ggatctgccc gaaccccgac cgcctcgatc
      961 agattcgtga cctcatgcgc caggtcatgt ccgatccggc cttctcgggc atcgccacgg
     1021 cttgcgacca gcagtacttc ccggtcgtct ctcccacgtc cgagttcgat cgctaccgga
     1081 tgtcgttcat cctcgtccat ggggcggtga ccaccgacat catctacggc tactcggtcg
     1141 gcgatggcga gcggcgcaag aaggtgcagg tcgagccgtc cccatacgcc cacgtcctgc
     1201 ctgagcgctt gccgccccgc gacctgatgc gccgcgccga ggaggcctat ctactgggcc
     1261 ggattctggg catcctgcgc ggggaaggcg cccgcaacga gggcgtgttt ccggggacgc
     1321 agacccggcc gcccacgcgg gtggatctcc accgtgactt gatgatcggc agtgtggacc
     1381 tgatccgccg ggccgaggga cggggcctgg agtgcttgga ggaggccgtc ctcgcccgca
     1441 tcgaggagct cgggcccgac tccgacgccc tcgcagaggt gctcttccgg tcattcggcc
     1501 agtgggaagg cgtggattcg ccccccgagg aactcatccg taacctgggc attgtgcgcg
     1561 tgcgggccgc actcgatgct gaggtgcccg gtgaggacga cgccgatctc gagatccaag
     1621 ccgtgcgcca tgtggtgctg cgattcctcg aacgccggaa cctgctccgc gatcgcatcg
     1681 aggccatgtc tcggcccgcc atctccaatg tgcgggagga gtacatcgac ggcgccaccc
     1741 agtgggtgtg cacgacctgc ggcgccccat accaccggcg ccgaccgctc tacggcgacg
     1801 tgtgtgagaa ctgcaacacc atgtttcact agtggtccag cac
//
LOCUS       DAOSPL010001074         1842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_63550_length_1842_cov_2.506435, whole genome shotgun sequence.
ACCESSION   DAOSPL010001074 DAOSPL010000000
VERSION     DAOSPL010001074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63550_length_1842_cov_2.506435"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PLQ54_16870"
     CDS             <1..558
                     /locus_tag="PLQ54_16870"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOS94991.1"
                     /translation="LHIRQNWPGRAAYVIGERGLTAELMEQGIPVVEHATDAGLVVVG
                     WDRGLTFDKLVQAHQAIVAGAVFVATNRDSSYPLSDGRTCPGAGALVASLVTSTGLEP
                     ECVGKPDPGGMRMIAMRWGVEPGQVAAVGDRLETDMAAARACGARAILVLTGIASRTD
                     GESAPPSMAPDVIIDDLSWLPEALK"
     gene            560..1351
                     /gene="thiD"
                     /locus_tag="PLQ54_16875"
     CDS             560..1351
                     /gene="thiD"
                     /locus_tag="PLQ54_16875"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOS94992.1"
                     /translation="MRAALAARPVVLTIAGSDSSGGAGVLADLRTIERHGCHGAAVIT
                     AVTAQTPGRVLDVEPVPIRVLESQLKATLTGLPVSAVKVGLLPTAAHVQAVAEALGRA
                     TVPVIIDPVAVASDGSALADPDVLPALLNELLPLSALVTPNALEVAALLGRATPTTAN
                     EQLTAARQLYARTGAAVLVKGGHVSGPRVVDVLADADGVAQFAHARVNTPFPVRGTGC
                     ILSSAIACRLTVGLRLRRAIRGARAHLRYRLLNAHRSGNDPVAIA"
     gene            1583..>1842
                     /locus_tag="PLQ54_16880"
     CDS             1583..>1842
                     /locus_tag="PLQ54_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018394879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOS94993.1"
                     /translation="MSKFIFVTGGVVSSVGKGITTASLGRLLKNRGLKVTLQKFDPYI
                     NVDAGSMNPYQHGEVFVTDDGAETDLDLGHYERFVDINLTKLA"
BASE COUNT          289 a          664 c          587 g          302 t
ORIGIN      
        1 ctgcacatcc gccagaactg gcccggccgc gccgcatacg tcatcggcga acggggcctc
       61 acggcagagc tgatggaaca gggcattccc gtcgtcgagc acgcgaccga tgcggggctc
      121 gtcgtcgtgg gttgggaccg cggcctcacg tttgacaagc tcgtgcaggc ccaccaagcc
      181 atcgtcgctg gcgccgtctt cgtggcgacc aaccgcgaca gctcgtaccc cctcagcgac
      241 ggccggacgt gccctggcgc cggcgccctc gtggcctccc tggtgacctc aaccggtctc
      301 gagccggagt gcgtgggcaa accagatccc ggcggcatga gaatgatcgc catgcggtgg
      361 ggtgtcgagc cgggccaggt ggctgcggtg ggcgaccggc tcgagaccga catggctgcc
      421 gcgcgggcct gcggtgcccg cgccatcctc gtgttgaccg gcattgcctc ccgcaccgac
      481 ggcgagtcag ccccgccctc catggcgcca gacgtcatca tcgacgatct ttcctggctc
      541 cccgaggcgc tgaagtgaga tgcgagccgc cctcgctgcc cgccccgtcg tgctcaccat
      601 cgcgggctcc gactcgagcg gcggcgccgg cgtgttggcc gatcttcgga ccatcgagcg
      661 ccacggttgc catggcgcgg cagtgatcac cgcagtcacg gcccagacgc ctggccgagt
      721 ccttgacgtc gaacccgtgc ccatccgcgt tctcgagtca cagctcaagg ccaccctgac
      781 cggcctgccc gtgtccgccg tcaaagtagg cctcctgccg acggcggcgc acgtccaggc
      841 ggtggcagaa gccttgggcc gcgcaaccgt gccggtcatc atcgacccag ttgccgtggc
      901 gtccgacggc tctgcgctgg ctgaccctga tgttctgccc gcgctgctga acgagctgct
      961 acccctatcg gccctggtga cccccaatgc cctcgaggtc gccgcattgc ttggccgggc
     1021 cacgccgacg acggcgaacg aacaactcac ggcagctcgc cagctgtatg cgcggaccgg
     1081 cgctgccgtc ctcgtcaagg gagggcacgt tagcgggccc cgggtggttg atgtcctcgc
     1141 cgacgccgac ggcgtcgccc agttcgccca cgcacgagtg aacacaccct tccccgttcg
     1201 cggcacggga tgcatcctgt cgagtgccat cgcctgtagg ctgaccgtcg gcctccggct
     1261 tcgacgggcg atccgcggcg ctcgggccca cctgcggtac cggctcctga acgctcatcg
     1321 atccggcaat gaccccgtcg cgattgcctg atgcattgcg gcgtgagccc ggtgcgagtt
     1381 cccccgtcga tgcgggtttt ggggactgcg gggggcacga ccgccggcga tggcagtgag
     1441 gcaatgcgga aaccgacgcc cgaggattgc ctggaggacc gactgcccct ccggcaaaca
     1501 taggtgatgg gctacgggcc ccgttgcgct ccgcacactc cccctcgcac agaaccctcg
     1561 ctccgcgaaa ggtttgtgtc caatgagcaa gttcatcttc gtaacaggcg gcgtcgtatc
     1621 gtcggtgggt aaggggatca ccacagcctc cctcggccgg ctgctcaaga accgcggtct
     1681 caaggtcacg cttcagaagt tcgacccata catcaacgtc gatgcgggta gcatgaaccc
     1741 ctaccagcac ggcgaagtct tcgtcaccga cgatggggcc gagaccgacc tcgatctggg
     1801 ccactacgag cgcttcgtcg atatcaacct cacgaaactc gc
//
LOCUS       DAOSPL010001075         1838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_63728_length_1838_cov_2.932698, whole genome shotgun sequence.
ACCESSION   DAOSPL010001075 DAOSPL010000000
VERSION     DAOSPL010001075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63728_length_1838_cov_2.932698"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="PLQ54_16885"
     CDS             complement(<1..1475)
                     /locus_tag="PLQ54_16885"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS94994.1"
                     /translation="MPDASGKAQIAIVGTGGIAGAHLQGYRKLLDAGYDSFEIVALCD
                     KFEDRRAAFAKTVSDMFGWTPPQYASVEEMVEKATLDGADICTPHAFHHTTVIPCLEA
                     GLPVMVEKPCGITIKASDLIIEAAKRAGKFVAVAEQVRRGIKARTMRWALCEKKMIGD
                     IRFFLVEGYSNWDFCSDAYKNAYAWQWRLLKLLTGGGMIFDAGAHFADMIRHMFGDVD
                     EVSCALRTFQRPILDSPELGPQPMDVEDTWMSCLRFASGLVGQWSWSFSAPGEGVAAQ
                     LFYGSEGSARDRGGWMHPFQQGGDIVFADGTTKPYEDIEKEYKAQLDPAEAARLFPHG
                     VEEDVALECWDFVTRFCRSGHLRSMPKRPKGPRPSAWPCMSRTWLARPSGCRMYTAAR
                     SAPIRTPSTSIGRSDRSERPTAIRAAGVRTSSRSGPRSSGETATGGTAMGEQPTDPQE
                     SIAEDREVLSALEHTKVYLECTREDRRIPGLSVAVVCDGATL"
     gene            complement(1598..>1838)
                     /locus_tag="PLQ54_16890"
     CDS             complement(1598..>1838)
                     /locus_tag="PLQ54_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOS94995.1"
                     /translation="CKFVARLFGREPNIEFASYNEMATLLGPAWAITRDHIDHSPCCS
                     IEKAQRLLGYAPRYTTEQIYIECLEYLLETGQLVV"
BASE COUNT          305 a          637 c          557 g          339 t
ORIGIN      
        1 agagtggccc catcgcacac cactgcgacc gatagaccgg ggatccgccg gtcttcacgg
       61 gtgcactcga ggtagacctt cgtatgctcc agtgccgaca gcacctcccg atcctccgcg
      121 atggactcct gcggatcggt tggctgctct cccatagccg tgcctccagt cgccgtctcg
      181 ccagacgatc ggggcccgct gcgcgaggag gtcctcacgc ccgcggccct gatcgctgtc
      241 ggtcgttccg acctatccga tcttccaata ctcgttgatg gggtcctgat aggcgctgat
      301 cttgccgctg tatacatcct gcaccctgac gggcgcgcca gccatgtccg actcatacat
      361 ggccaggcag atggccttgg cccttttggt cgtttcggca tcgatctcag gtgcccgctg
      421 cgacagaatc gagtcacgaa gtcccagcat tcgagtgcga cgtcttcctc gacaccgtgc
      481 gggaacagcc gggccgcctc ggccgggtcc agctgggcct tgtactcctt ctcaatgtcc
      541 tcatacggct tcgtggtgcc atcggcgaac acgatgtcgc cgccttgctg gaaggggtgc
      601 atccacccgc cccgatcccg ggcactgccc tcggagccat agaacagctg ggcggcgacg
      661 ccctcgccag gcgccgagaa cgaccacgac cattgcccca cgagtccgct ggcgaaccgc
      721 aggcacgaca tccaggtgtc ctcgacgtcc atgggctgcg gacccagctc gggcgagtcc
      781 aggatcgggc gctggaaggt ccgcaaggcg caactgacct catccacgtc cccaaacatg
      841 tggcggatca tgtccgcgaa atgggctccc gcgtcgaaga tcatgccacc acccgtgagg
      901 agcttcagca ggcgccactg ccacgcgtag gcgttcttgt aggcgtcgct gcagaagtcc
      961 cagttcgagt agccctcgac caggaagaac cggatgtcgc cgatcatctt cttctcgcac
     1021 agagcccagc gcatggtccg cgccttgatg ccgcgccgaa cctgttcggc cacggccacg
     1081 aacttcccgg ctctcttggc ggcctcaatg atcaggtccg atgccttgat ggtgatcccg
     1141 cacggcttct ccaccatcac cggcaacccg gcctcgagac acgggatcac ggtggtgtgg
     1201 tggaacgcat gcggcgtgca gatgtccgcg ccatccagtg tggccttctc gaccatctcc
     1261 tccaccgacg cgtactgcgg cggcgtccag ccgaacatgt cgctgaccgt cttggcgaag
     1321 gccgctcgcc gatcctcgaa cttatcgcac agggccacga tctcgaatga gtcgtacccg
     1381 gcgtccagca gcttgcggta accctgcaga tgggcacccg cgatgccgcc ggtccccacg
     1441 atggcgatct gggccttgcc cgaagcgtca ggcatggatg ttccactccc tgtggtcctc
     1501 atagatggcc caaggccgga gcctacgtgc ggtcggccgg ggcactgtca gcgagtattc
     1561 ccgccgcgcg ccgagggtcc ttcgggcccc cgattggcta gaccacaagc tgccccgtct
     1621 cgaggaggta ctcgaggcac tcgatgtaga tctgctccgt cgtgtagcgc ggcgcgtagc
     1681 ccagcaggcg ctgggccttc tcgatgctgc agcagggcga gtggtcgatg tggtccctgg
     1741 tgatcgccca cgcggggccg aggagcgttg ccatctcatt gtacgaggcg aactcgatgt
     1801 tcggctcgcg tccgaacagc cgcgcgacga acttgcag
//
LOCUS       DAOSPL010001076         1837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_63782_length_1837_cov_2.556678, whole genome shotgun sequence.
ACCESSION   DAOSPL010001076 DAOSPL010000000
VERSION     DAOSPL010001076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63782_length_1837_cov_2.556678"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLQ54_16895"
     CDS             <1..196
                     /locus_tag="PLQ54_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011243455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOS94996.1"
                     /translation="SHLANVLDAKTLVIHPASTTHQQLTEDEQLQAGVTPDMVRLSVG
                     IESADDIMADLDQALRASQV"
     gene            302..>1837
                     /locus_tag="PLQ54_16900"
     CDS             302..>1837
                     /locus_tag="PLQ54_16900"
                     /inference="COORDINATES: protein motif:HMM:NF039329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94997.1"
                     /translation="MRVALTVAAATLWAPIGQAAAAEVTLTLASDLPFDRVGEPVTCG
                     VPLVHGFARGPEELALLGPSGNPLPVQVETTSAFRDGTPRWVLLRFAADVPAAGRAVY
                     RLTQGRPTALPQPLTCELNGGVATIDTGAARFTIDTRRFHLFDSVEVGGQSVIALGGG
                     AFLDWADGARTMRVEDVVSAEFEQAGPLAAVLCVRGSIGPADPLPLADYVCRIEFRAG
                     SSEARVTYTLHNPAAHVHPGNTWDLGTGGSVFIEDFSLVVPLPDGAWTSRVGVGGGMP
                     PVAATKLYQDSSGGPNWNSANHVDQDMRVPMSFRGYRVFGGDEVIGEGQRAEGWLHAR
                     RDGAGGIAVSVRDFWQNCPKALERPGDSVRIGLWPREFAGAHELLGGEQKTHEALFVF
                     HGAEVTDEAVQHRMEAFQHPLYAMPDPEAVLATRAFAPSAPLDRERFAALEATCDAFV
                     EPAAGGESVFSKWDQIDEYGWRHFGDIWGDHEAVYHHGPAPMVSHYNNQYDFIFAAGI
                     QFLR"
BASE COUNT          280 a          615 c          647 g          295 t
ORIGIN      
        1 ctctcacctc gccaacgtgc tcgatgcgaa gacactggtc atccatccgg ccagcaccac
       61 tcatcagcaa ctcaccgagg atgagcagct ccaggccgga gtcacgccgg acatggttcg
      121 cctctcggtg ggcatcgagt cggccgacga catcatggcg gacctcgacc aggccctgcg
      181 ggcatcgcag gtctagatca ctggactgcc ggccgagcag aagcagggct gagccgcgtc
      241 agggcgagac cgcccggtag ggggggtggc gaccacgagc catggaatga gggacgccca
      301 catgagagta gcgctcaccg tcgcggcggc aacgctgtgg gcaccgatcg ggcaagccgc
      361 cgcggccgag gtgacactga ccctcgccag tgatctgccc ttcgaccgag tgggtgagcc
      421 ggtcacgtgc ggcgtgccgc tggtgcacgg gttcgcccgc gggccggagg agctggctct
      481 gctcggccca agcgggaacc cgttgccggt gcaggttgag acgacgagcg ccttccgtga
      541 tggcacgccg cggtgggtgt tgctgcgctt tgcggccgat gtgccggctg ctgggcgggc
      601 cgtctaccgg ctgacccagg gccggcccac tgctctgccg cagcccttga cctgcgaact
      661 caacggtggg gtggcgacga tcgacacggg tgccgcgcgg ttcaccattg acacccgccg
      721 gtttcacctg ttcgactcgg tcgaagtcgg cgggcagagc gtcatcgccc tgggcggagg
      781 cgcattcctc gactgggccg atggggcccg cacgatgcgg gtcgaggacg tcgtctcggc
      841 cgagttcgag caggccggcc cgctcgcggc cgtgctgtgc gtgcgcggca gcatcggacc
      901 ggcggacccc cttccgttgg ccgactacgt gtgccgcatc gagttccggg cgggctcgag
      961 cgaggcgaga gtcacctaca ccctccataa ccccgcggcc catgtgcatc cggggaacac
     1021 ctgggatctc ggcaccggcg ggtcggtatt catcgaggac ttctcgctgg tcgtgccact
     1081 acccgatggc gcctggacct cacgcgtggg tgtgggagga gggatgccgc ctgtcgccgc
     1141 cacgaagctc taccaggact cctccggtgg gccgaactgg aactcggcca accatgtcga
     1201 ccaggacatg cgggtgccca tgagcttccg aggctaccgc gtctttggcg gcgatgaggt
     1261 gatcggtgag ggccagcgcg ccgagggctg gctccatgcg cgacgcgacg gcgcaggcgg
     1321 catcgcggtc tcggttcgtg acttctggca gaactgcccg aaggccctcg agcgacccgg
     1381 cgatagcgtt cgcatcggcc tgtggccgcg cgagttcgcg ggcgcacatg aactgctcgg
     1441 cggcgagcag aagacccacg aggcgctgtt tgtcttccat ggcgctgagg tgacggacga
     1501 ggccgtgcag caccgtatgg aggccttcca gcatccgctc tatgcgatgc ccgacccgga
     1561 ggccgtgctg gcgactcggg ccttcgcgcc ctccgcgccg ctcgaccgcg agcggttcgc
     1621 cgcgctcgag gccacctgcg acgctttcgt cgagccggct gccggcggcg agagcgtctt
     1681 tagcaagtgg gaccagatcg acgagtacgg ctggcggcac ttcggcgaca tctggggcga
     1741 ccacgaggcg gtctaccacc acggcccggc gccgatggtg tcgcactaca acaaccagta
     1801 cgacttcatc tttgcggcgg gcatccagtt tcttcgt
//
LOCUS       DAOSPL010001077         1835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_63860_length_1835_cov_3.535393, whole genome shotgun sequence.
ACCESSION   DAOSPL010001077 DAOSPL010000000
VERSION     DAOSPL010001077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63860_length_1835_cov_3.535393"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..215
                     /locus_tag="PLQ54_16905"
                     /pseudo
     CDS             1..215
                     /locus_tag="PLQ54_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491447.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            1206..>1835
                     /locus_tag="PLQ54_16910"
     CDS             1206..>1835
                     /locus_tag="PLQ54_16910"
                     /inference="COORDINATES: protein motif:HMM:NF025226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOS94998.1"
                     /translation="MPSAVFDASARLVCMAGFAAVGVMLAVALIYRLARPRKDLHHAA
                     AHGAVGVMTQLLDAGVPVDRTDDIGRTPLHNAVTACQRAAAELLLDRGADVRAADKDG
                     FTPLHLACYEGHADLAELLLDRGADIEGPAGGYGHHPLHSAAARGRTRVVRLLLDRGA
                     DIEARDAEGRTALHLAADTGFADLVRALLDAGADRQAEDDEGLTPRDRAL"
BASE COUNT          248 a          627 c          677 g          283 t
ORIGIN      
        1 gcctctgcac cgtgctggcc tcggactatt actatcccgc gctgctgcag gcgcccttcg
       61 tgctcgcgcg caacggcacg ctgccgctcg agcgtgcctg gcggctggtc tcgcgggcgc
      121 cggcggaagc cttcgaactg tccgaccgcg gcgagatcgc gcccggccgg cgcgccgatc
      181 tggtgcggct cctcggtgag gagtccgaac cgctcgccga actgctgggc gttggcgaag
      241 ccacccgtca gctcgcggac ggcgctctcc gagatcttga agtaggcgtc gagtacgctg
      301 gactcctcgg gcgaggcgca cacgatcagg gactggctga gcagcggccc cgaggcgtac
      361 aggtgccgcc gagcgtccag cacggacatg cggtcatcca gcaggagccc caggatgtcc
      421 ttgccacggg agtaggttcg cgccccactc acactgatgt cctcgaggag cagtaccagc
      481 aggatgtgga gttcgagctc gcacaggccg taggtctcgg ccatgcgctc gatgctgagc
      541 cgggcgccct gtgcgcgggc gagagcggcc ttctgcgcga cccggcgctc gagggaccgc
      601 acttcgctct ggaggcggcg gatccgcgcc tggcgggcgc gttcgtcttc gtcatcgctg
      661 cgggcgggcc ggtcgaaggc ttcgcccatc cagtcgccca tccgcgtctt gattgtgtac
      721 agctcctgct tggtctcgac caggcggagc aagtcgcgga ggtggtcctt gtcattccgg
      781 tagggattcg tgatctttgc gcccatagat ggcctggcgc ttcgacccag caagccactc
      841 gcctgcctgc ggcacccggg ctggtacgcg ggctggcgac gggctgtcga aggctctccc
      901 gacccccttc agtacggtgg ctccatgcca gaggcctggc ggccaggcag ccgaagaccg
      961 gatcgagaga gggaggcgct catactgcca gcaggcatcc gggagggctg aatccggagc
     1021 gcgcttgcgg tgccaacggg ccgggaaagc tcgcggtgtg cgatcgggcg ccccccctgc
     1081 tgtggcagcc tctcccccca cctcgtcggt cttcgatggg caaacgctga ggcaccgggc
     1141 ggagttcaag gccgggggcc ccgtcggcgg ctataatggg ggccgcgcga agagaggggt
     1201 atggcatgcc cagcgccgtg tttgatgcgt cggcccggct ggtgtgtatg gcggggttcg
     1261 ccgcggtcgg ggtcatgctg gcggtggcgc tgatctaccg cctggcgcgg ccccggaagg
     1321 acctgcacca cgccgcggcc catggtgccg tgggcgtgat gacccagctt ctggatgccg
     1381 gtgtgccggt ggaccggacg gatgatatcg ggcgcacgcc cctccacaac gcggtcacgg
     1441 cgtgccagcg ggcggccgcg gagctgctcc tcgatcgcgg cgcggacgtg cgcgcggccg
     1501 acaaggatgg gttcacgccg ctgcacctgg cctgctatga gggccacgcc gacctggccg
     1561 agctgctcct ggaccgcggc gccgacatcg aggggcccgc gggcggctac ggccaccacc
     1621 ccctgcactc ggccgccgca cggggacgga cccgggtggt gcggctgttg ctcgatcgcg
     1681 gggccgacat cgaggcgcgc gatgccgagg gacggaccgc gctgcacctg gccgccgaca
     1741 cgggcttcgc cgacctggtt cgcgccctgc tggacgccgg cgccgaccgg caggcggagg
     1801 atgacgaagg actgacgccg cgcgaccgag ccttg
//
LOCUS       DAOSPL010001078         1835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_63871_length_1835_cov_3.062360, whole genome shotgun sequence.
ACCESSION   DAOSPL010001078 DAOSPL010000000
VERSION     DAOSPL010001078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63871_length_1835_cov_3.062360"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1716
                     /locus_tag="PLQ54_16915"
     CDS             <1..1716
                     /locus_tag="PLQ54_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS94999.1"
                     /translation="SQYVSQLLSGAARPSRRRVQEIERALQLGVGSLTQEGPALPEGL
                     ETPERVLAPDFAAWEELLASARRLRHDGHLGTALIVANQLVERLSAVRTTSERVAGLF
                     ANALILRSELHRCQCAPDMALEDAARARRLATPLKASDPGLFSRAYFWHIIRAQDLRG
                     ASGLAEVVTDAFPEGPDPAMALLPVTAEAFIADGGAGVTPELSARLAGAIEGAPFSPA
                     KSVAFLMLQTRHDRGDEFGDRWTDEARRAGEVVGLAEALYHAAHRAFRSGRPLDAYRH
                     LLESTQIAASAEFQLGMEHAAEVWSQMLRAPALERAEASGVPRELLRGEIVVPACQLL
                     REVGLRSEWFGTARAFAWAACAHGSAALGDPAGALEAAGWLLPMARRAFNADNAAANL
                     AWAAWALQKSGFTDEATAMWSHVRSATGLALAEGSRVPHERLSEMAYAAMEAGHGLPE
                     LDSVMRHIATQQPTDAWAWGLNARYQLSRGQVLDALAAAERSWRLAQYLGLAQAFPPL
                     PSYRAPLGRVQRQQAARREAMAWTAEAFARALELDSLSPSTRESISRSLAALQSECAM
                     VSAPE"
BASE COUNT          250 a          605 c          664 g          316 t
ORIGIN      
        1 agccagtatg tctcccagct gttgagtggc gctgcacgac cctctcgccg tcgagtgcag
       61 gagatcgagc gcgccctcca gttgggggtt ggcagcctga cccaggaggg tcctgccctg
      121 cccgaagggc tcgagacacc cgaacgggtg ctggcgccgg acttcgccgc ctgggaggaa
      181 ctgctcgcca gcgcccggag gcttcgtcac gatgggcacc tgggtacagc cttgatcgtg
      241 gccaaccaac tggtcgagcg tctctcggcg gttcggacga cgagcgagag ggttgccggg
      301 ctgtttgcga atgctctgat cctgcggtcg gagcttcacc ggtgccagtg cgccccggac
      361 atggcgctgg aagacgcggc gcgcgcccgt cgcctggcca cgccactgaa agcgagcgac
      421 cccggcctct tctcccgggc ctacttctgg cacatcatcc gggcacagga tcttcgcggg
      481 gcgtcgggac tggcagaggt ggtcacggac gcgttcccgg agggcccgga tccggccatg
      541 gctttgcttc ccgttactgc cgaggcattc atcgccgatg gcggggccgg ggtgacgcct
      601 gagctgtcgg ctcgcctggc cggggccatc gagggagcac cgttctctcc ggccaagtcc
      661 gtggcgtttc tcatgctcca gacgcgccac gaccgcggcg acgagttcgg cgatcggtgg
      721 acggacgagg ctcgccgagc cggtgaggtc gtggggctgg ctgaggcact ctaccacgcg
      781 gctcatcgcg ctttccgctc gggccggccg ctggatgcat accgtcacct tctggagtcg
      841 acccagattg ccgcatccgc ggagttccag cttggtatgg agcacgcggc cgaggtgtgg
      901 tcccagatgc tccgcgcccc cgcactcgag agggccgagg cgtcgggggt gccccgggag
      961 ttgctgcggg gcgagatcgt cgtgccggcg tgtcagctac tgcgcgaggt ggggttgcgg
     1021 tccgagtggt tcggtacggc gcgcgccttc gcgtgggcgg cgtgcgcgca cggtagtgcg
     1081 gcgctgggtg acccggccgg tgccctcgaa gctgccggct ggcttctgcc catggcacgg
     1141 cgggccttca acgcggacaa tgccgccgcg aacctggcgt gggcggcatg ggcgctgcag
     1201 aagtcgggct tcaccgatga ggccacagcc atgtggtcgc acgtccggtc agctacgggg
     1261 ctggcgctgg ccgaaggctc gagagtcccc catgaacggc tgtcggagat ggcctatgcg
     1321 gccatggagg cggggcacgg cctcccggag ctcgactccg tgatgcgcca tattgcgact
     1381 cagcaaccga cggatgcatg ggcatggggc ctcaacgcgc gctaccagct ctccaggggt
     1441 caggtgctcg acgccctggc agcggcggag cgcagttggc ggctggctca gtaccttggg
     1501 ctggcgcagg cctttccccc gctcccgagc tatcgggcgc cgctgggccg cgtccagcgg
     1561 cagcaagctg cccggcgcga ggccatggcg tggaccgctg aggccttcgc ccgagcgctg
     1621 gagttggaca gcctctcccc ctccacccgt gagtcgatct cgcggtcgct ggccgcactt
     1681 cagtccgagt gcgccatggt gtctgcgccc gagtgagtgg gcccgggtta ccatgactta
     1741 cgggttgcgg cgatcgccat tgacgcgctc tagcagatca gttaacttag aatagggcgc
     1801 aagagagcgc tttcagccgc ttggtgctcc cctcc
//
LOCUS       DAOSPL010001079         1834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_63914_length_1834_cov_3.725126, whole genome shotgun sequence.
ACCESSION   DAOSPL010001079 DAOSPL010000000
VERSION     DAOSPL010001079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63914_length_1834_cov_3.725126"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            421..1305
                     /gene="lipA"
                     /locus_tag="PLQ54_16920"
     CDS             421..1305
                     /gene="lipA"
                     /locus_tag="PLQ54_16920"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOS95000.1"
                     /translation="MAAQRPPEWLRVKLGKLEHSRQTAQRLARHGVSTVCSNARCPNI
                     GECYGGGTATFLIMGEVCTRDCAFCAVRHGAPQPLDPAEPQRVAEAAADLGLTHVVVT
                     SVTRDDLPDGGAAHFVATVGAIRQALPHATIEVLTPDFGGAPEPTALVARAAPDVLNH
                     NLETVRRLQRSIRPQASYETSLDVLRRFREIAPTAATKSGLMLGLGETEEEVLEAMAD
                     LRRVGCDLLTLGQYLRPTPRHTAIDRYVHPDEFERLRARAEAMGFIHCASGPFVRSSY
                     DAATAAQVVASLRAEVRG"
     gene            1302..>1834
                     /locus_tag="PLQ54_16925"
     CDS             1302..>1834
                     /locus_tag="PLQ54_16925"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOS95001.1"
                     /translation="MTLGPVMAVVCLSQAALGLVQLDTSAFPRVTLSLDQSLPADVPA
                     EDGTPVAPVADAPAPGPWHLAVVIDSSASTEPYAKALRVAVIDVARLLSGVAELAVVD
                     AGSQPRVLCGFTPHSADVVAALRQVVPNGGSALWDGLVAALGVAADAPGRRAVLLITD
                     GRHEGGLAELDDVLLLAR"
BASE COUNT          259 a          658 c          622 g          295 t
ORIGIN      
        1 gtagtcatag tcgaccgggt cacccaggaa ctccaggacc gacgagaggg agccggggaa
       61 ggggcacatg tgaacaccga agccgatctt cgggacgccc tcgatcacgg ccggcgcagg
      121 atatgcgtcc gccaccgtga catcccccct cggccccccg gagccaacac ggtggagcca
      181 gaacggccaa catggccgcc acaccgtagg caaccgctct gcctcgcacg gtcaccactt
      241 ccgggagtgg cgggattatg cgcgcgcctg cggacggcgt caacggcgcg ccgtagcgtg
      301 caactgcggg tcctcggaag ggcgcggtgg ggcggcgttg tgtggcgcca ggtcgggccg
      361 cgggtccctg gcgtatactg gaatgggtcc atttccccgg gaacggagtg accgagacgc
      421 atggcagctc agcggccacc tgagtggctc cgcgtgaaac ttgggaaact ggagcactcg
      481 cgccagaccg cccagaggct ggcccggcac ggcgtgagca cggtctgctc gaacgcccgg
      541 tgcccgaaca tcggcgagtg ctacggaggc ggcaccgcga ccttcctgat catgggcgag
      601 gtctgcacac gcgactgcgc cttctgcgcc gtccgccacg gcgcgcctca accccttgac
      661 ccggcggagc ctcaacgtgt cgccgaggcg gcagccgacc tcggccttac ccacgtggtc
      721 gtcacgtcgg tgacgcgcga tgatctcccc gatggcggtg cggcccactt cgtggccacg
      781 gtgggcgcca tccgccaggc cttgcctcat gccaccatcg aggtcctcac tcctgacttt
      841 ggtggcgcgc cggagcccac cgctctcgtc gcgcgggcgg cgcccgatgt gctcaaccac
      901 aacctggaga ccgtgcggcg gctgcagcgc tcgattcggc cccaagcctc ctacgagacc
      961 tccctggatg tcctgcgccg gttccgcgag atcgcgccca ccgcagcgac gaaatcgggc
     1021 ctcatgctcg gcctcggcga gaccgaagag gaagtgctcg aggccatggc cgacctgcgt
     1081 cgtgtgggct gcgacctcct gaccctcggc cagtacctgc ggcccacgcc ccggcatacc
     1141 gccatcgacc gttacgtgca tcccgatgag ttcgagcggc tccgcgcccg ggctgaagcc
     1201 atgggcttca tccactgtgc ttcggggccg ttcgtccgca gctcctacga cgcggctacg
     1261 gccgcgcagg tggtggcgtc actccgagcg gaggtacgtg ggtgacgctg gggccggtca
     1321 tggcggtcgt ctgtctgagc caggccgctc tgggcctggt tcagctcgac acctcggcgt
     1381 tcccgcgggt gacgctgtcg ctcgatcagt ccctcccggc cgacgtcccg gccgaggatg
     1441 gaacgccggt cgctccggtg gccgatgcgc ccgcgcccgg cccctggcac ctggccgtgg
     1501 tgatcgactc atcggccagc accgagccct acgccaaggc gctccgcgtg gccgtgatcg
     1561 acgtggcccg tctgctatct ggcgtggccg aactcgccgt tgtcgatgca ggctctcaac
     1621 cgcgtgtact ctgcgggttc accccacact cggcggatgt ggtcgctgcg cttcggcagg
     1681 tggtccccaa tggcggttct gccctctggg atggcctcgt cgcggctctg ggcgtcgccg
     1741 ctgatgcgcc ggggcgccgc gccgtcctgc tcattaccga tggccggcat gagggaggtc
     1801 tggccgaact cgacgacgta ctgctgctgg cccg
//
LOCUS       DAOSPL010001080         1834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_63933_length_1834_cov_2.738617, whole genome shotgun sequence.
ACCESSION   DAOSPL010001080 DAOSPL010000000
VERSION     DAOSPL010001080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63933_length_1834_cov_2.738617"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLQ54_16930"
     CDS             complement(<1..197)
                     /locus_tag="PLQ54_16930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95002.1"
                     /translation="MPNFTPTTFRQRALSFVGLPYILGAEFDSSFPSTDKPRALDCSE
                     LTETLCRENGTPLGDHSHDRGS"
     gene            complement(272..535)
                     /locus_tag="PLQ54_16935"
     CDS             complement(272..535)
                     /locus_tag="PLQ54_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95003.1"
                     /translation="MQIVLAAVTSGFLVKVLDVLISARAQARKARREAELEPETELQA
                     ARASRIWWMEHAANARAAAAQAGIPLSPLDLRADPYPPKWAIQ"
     gene            complement(551..1021)
                     /locus_tag="PLQ54_16940"
     CDS             complement(551..1021)
                     /locus_tag="PLQ54_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95004.1"
                     /translation="MTAIYVACYVIAVLGGLAIITGSAQTVLPDGWDKLEGVILIIAG
                     TVGAPAAWMGRWWAERAPVLLMVLTGLTLAILAIFVTLTPELPPVIDTTRSPSWALGI
                     AFWLALFITRWALGVGRHPYAPGRGPATMATREAQAKRVMQHCERGDGLHENRG"
     gene            complement(1112..1630)
                     /locus_tag="PLQ54_16945"
     CDS             complement(1112..1630)
                     /locus_tag="PLQ54_16945"
                     /inference="COORDINATES: protein motif:HMM:NF019126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HOS95005.1"
                     /translation="MLIVGLLGGSDSTPVATILPYGSGEIPQGYLLCNGQDVSRAAYA
                     DLFSKIGTTYGAGDGSTTFSVPNLRGRVVVGHSAAEPEFNNLGQRAGEKAHTLTMGEI
                     PAHGGHIDGYTGTLAAYLAAPGGLTSYTDTAHGWKQYRGNEIYPYHFDVGGGGSHNNL
                     PPYLVGHYIIRT"
BASE COUNT          306 a          565 c          629 g          334 t
ORIGIN      
        1 gaccctctgt cgtgagaatg gtcgccgagc ggcgtaccat tctcacgaca gagggtctcg
       61 gtcagctcgg agcagtcgag ggctcgcggc ttatccgtgc tcgggaagct agagtcaaac
      121 tcggcgccga ggatgtaggg aaggccaaca aagctcaggg ctcgctggcg gaaggtggtg
      181 ggagtgaagt tgggcaaggt gcctccttga gttgtgaatg ctttggacgc gaatgagccc
      241 cgtcacagtc ttgttggctt tgttcgtgtg actactggat cgcccacttg ggcgggtatg
      301 ggtccgcccg aaggtccagg gggctaagtg gaatacctgc ctgagcggct gccgcgcgag
      361 cgttggcggc gtgttccatc caccagatgc gtgaggctcg cgcggcctgg agctccgtct
      421 ccggctctag ctctgcctct cggcgcgcct tgcgcgcctg tgcccgggcg gagatcagga
      481 cgtcgagaac cttgaccagg aagcccgacg tgacggcggc gaggacgatc tgcatcgtga
      541 catccatggg ctacccccga ttctcgtgga gcccatcgcc cctctcgcag tgctgcatca
      601 ctcgtttggc ttgcgcctct cgcgtggcca tggtggccgg ccctcgtccc ggggcgtacg
      661 ggtggcgacc aacgccaagc gcccagcggg tgatgaacag cgccagccag aaggcgatcc
      721 cgagcgccca cgagggggac cgtgtcgtat cgatcacggg cgggagttct ggggtgaggg
      781 tgacgaagat ggccaggatg gcgagggtca acccggtcag gaccatgagc aggacgggtg
      841 cccgctcagc ccaccaccga cccatccatg cggctggggc accgaccgtg ccggcgatga
      901 tgaggatcac cccctccagc ttgtcccacc cgtcaggcag gacggtctgg gcggagccag
      961 tgatgatcgc cagtccgccc aggactgcga tcacgtagca ggcgacgtag atcgcagtca
     1021 ctcggcgagg ctcgtgcagc gtgctccagg cggctcccag tgctgagcga ctcacgttga
     1081 tcgctccatg tattcagtca tcgctggcct cctaggtgcg aatgatgtag tggccgacca
     1141 agtacggcgg caggttgttg tgcgacccgc ccccgccaac gtcgaagtgg taagggtaaa
     1201 tctcgtttcc gcggtactgc ttccagccgt gcgcggtgtc cgtgtatgag gtcagcccgc
     1261 cgggggcggc caggtaggca gcgagcgtgc ccgtgtagcc gtcgatgtgt ccgccgtgcg
     1321 ccggaatctc gcccatggtc agcgtgtgcg ccttctcgcc ggcccgttgt cccaggttgt
     1381 tgaactcagg ctcggcggcg ctgtgcccaa ccaccaccct cccgcgcagg ttggggacgc
     1441 tgaaggtagt ggagccatct cccgctccgt aggtggtgcc aatcttggag aacaggtcgg
     1501 cgtaggccgc gcgcgaaacg tcctgcccgt tgcaaagcag gtacccttgt ggaatctcgc
     1561 cagagccgta cggcaggatc gtggcgaccg gggtggagtc gcttccgcca aggaggccga
     1621 cgatcagcaa ggtcttaccc tgcaaggtgg cggcgaagcg cctcccccgc tcaagtctga
     1681 cgaggctggg cggggctccg tcaagcgggg tgtcgctggc gtctagatac acttgaccgg
     1741 tggagctgat cgagccgaat agaagctcgg acccgccact gggtgccatc accatgtcga
     1801 ggaggtggtc taatccatcc acttcgtcac ctca
//
LOCUS       DAOSPL010001081         1833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_63985_length_1833_cov_2.804837, whole genome shotgun sequence.
ACCESSION   DAOSPL010001081 DAOSPL010000000
VERSION     DAOSPL010001081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63985_length_1833_cov_2.804837"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PLQ54_16950"
     CDS             <1..417
                     /locus_tag="PLQ54_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95006.1"
                     /translation="MHMTRRGFAVMVLVSLWCAVFLGLPAAAERRTPPPPPKEEPLTV
                     VLRADSIVSPAPVAEGAKRIVYVTTGVRIKLTVKSEDKTYKFSIEGKSGSAVTVEKNK
                     AISPVYFQFTNAGEEHEVLLYVGETKVSSHLFFRAK"
     gene            616..1017
                     /locus_tag="PLQ54_16955"
     CDS             616..1017
                     /locus_tag="PLQ54_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS95007.1"
                     /translation="MASEDRARKRILAVDDEVHMTKLISLHLEKAGYEVTVASDGAQA
                     LEMFDRINPDLVVLDIMMPILDGWGVLKALREDANRAQVPVIMLTSRSQDADITRGWQ
                     EGVDSYLTKPFNPLELVTLVRRILDEEEEAD"
     gene            1062..1568
                     /locus_tag="PLQ54_16960"
     CDS             1062..1568
                     /locus_tag="PLQ54_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95008.1"
                     /translation="MSDRPPPVAKKAGVSLGAIVTLVVAFLVLCVGMYYADTLRGVLF
                     LRPWSKAGPIGAIRAYAKALEANDEAALDAVGGSVGFQCVFEGGRIVALKSGGAFGSF
                     PPIPVEQATPSSFTPDQDSYYRYTADPPVYTVMLPAKDGGRLSFGMTRVNGKWAIISL
                     NPIGAGKQ"
     gene            1638..>1833
                     /locus_tag="PLQ54_16965"
     CDS             1638..>1833
                     /locus_tag="PLQ54_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95009.1"
                     /translation="MGDADTQQLGSPESQQESQPAAAAKRPPVSRDAVVVLVLGLVLL
                     LPLLYFSEAIVGAVKLQVWSK"
BASE COUNT          307 a          539 c          644 g          343 t
ORIGIN      
        1 atgcacatga cacgcagagg cttcgccgtc atggttctcg tctccctgtg gtgtgcggtc
       61 ttcttgggcc tgccggcggc cgcggagcgg cggacgccgc ctcctccccc caaggaggag
      121 ccactgacgg tcgtgctccg ggctgactcg attgtgagcc cggccccggt cgcggagggc
      181 gcgaagagga tcgtctacgt gacaacgggc gttcggatca agctcacagt caagagcgag
      241 gacaagacgt acaagttctc gattgagggt aagtccggct ctgcggtgac ggtggagaag
      301 aacaaggcga tcagccccgt gtacttccag tttaccaatg cgggcgagga gcacgaggtg
      361 ctgctgtacg tcggagagac gaaggtcagt agtcatctct ttttcagggc caagtgatcg
      421 cttctcctag cgggcctggt atggctggcc ggagttgcgc gaccgtcctc tccctggtgg
      481 ctcgttccgc cagggagttg ctgttggctc cgagtgcacc ggtgtgtggc cggggaaggg
      541 ctggccagcg gtgtgccgaa gataggagag cgaccagcca gcagagcccc gtggctctcc
      601 agggaggacc gtcccttggc ttcggaggat agggcacgca agcgcattct ggccgtggat
      661 gatgaggtcc acatgaccaa gctcatctcg ctccatctgg agaaggcggg ctacgaggtc
      721 accgtggcgt ccgatggtgc gcaggcacta gagatgttcg accggatcaa ccccgatctg
      781 gtcgtgctgg acatcatgat gcccatcctc gatggctggg gagttttgaa ggcgctgcga
      841 gaggacgcga accgggccca ggtaccggtg atcatgttga cgtcgcgttc gcaggacgcc
      901 gacatcaccc gcggctggca ggagggtgtg gacagctatc tcaccaagcc gttcaaccct
      961 ctggagctgg ttaccctggt tcgacgcatt ctggatgagg aggaggaggc cgactagaag
     1021 tcgcccccgc cctcccggga ttcgctgagg agaggaccgc ggtgtcggac cgccccccgc
     1081 cggttgccaa gaaggccggt gtcagtttgg gcgcgatcgt gactttggtc gtcgcctttc
     1141 tggttctgtg cgtcggcatg tactacgcgg acacgcttcg aggagtgctg ttcctgcggc
     1201 cgtggtcgaa ggcgggcccc atcggagcga ttcgggccta tgccaaggca ctggaggcca
     1261 acgacgaggc cgcgctggat gccgtcggtg gcagtgtggg tttccagtgc gttttcgagg
     1321 gtgggcgcat cgtcgcgctc aagtcgggcg gcgcgtttgg ctccttcccc ccgattccgg
     1381 ttgagcaggc cacaccatcg agcttcactc cggaccagga ctcgtactac cggtacacag
     1441 ccgaccctcc cgtctacacg gtcatgcttc ccgccaagga tggtggccgg ctctcgtttg
     1501 gcatgacgcg ggtgaacggc aagtgggcca tcatcagcct caacccgatc ggggccggca
     1561 agcagtaggc agagaggcgc acgatcgggt ggcgccgcgt cgagccgtga acgaccacag
     1621 tcagggagcc gaccagcatg ggggacgcgg acacgcagca gttgggctct cctgagagcc
     1681 agcaggagtc gcagcccgcg gcggcagcga aacggcctcc cgtgagccgg gacgcggtcg
     1741 tggtcctggt gttgggtctc gtgctgctgc tgcccctcct gtacttctcg gaggcgattg
     1801 tgggtgccgt gaagctgcag gtgtggtcga agg
//
LOCUS       DAOSPL010001082         1832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_64009_length_1832_cov_4.258863, whole genome shotgun sequence.
ACCESSION   DAOSPL010001082 DAOSPL010000000
VERSION     DAOSPL010001082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64009_length_1832_cov_4.258863"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PLQ54_16970"
     CDS             <1..956
                     /locus_tag="PLQ54_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95010.1"
                     /translation="GGIIIDVARSGQTLFPVPDSPYITRAGFEDGTQLTRWIDEIHAQ
                     GLKAYAGVNLLRWWLLFSEDPNPFERVPELLELDSSLSCSDELDAKYASMWHPKVRAG
                     LVALMRELATRYPGFDGLYLQAQFSRTAYLGFSDSARIAYIRHAHIDPVDLPLYGVDE
                     FQDKYLLAYFEWRPKQLTELVREVVGAYREIAGADDRVIAMATATGPARRPRHRATPG
                     DDWVTWLLEGLASDLAFEASVWLDGWPGMFRAGLDTFGRLRTPAPTYLIVSGEQRGVL
                     SPIADVYSALSQGDISGLPLLLSPYAPAQLDGSLKLLRSLR"
     gene            985..1200
                     /locus_tag="PLQ54_16975"
     CDS             985..1200
                     /locus_tag="PLQ54_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95011.1"
                     /translation="MATDDAETRTSRRGFLRSSAGMSLAAFVALPTMDAVVSAVTDRL
                     GERALGRQLAETLRTTEAARSESVCEA"
     gene            1281..1685
                     /locus_tag="PLQ54_16980"
     CDS             1281..1685
                     /locus_tag="PLQ54_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95012.1"
                     /translation="MRISSTLLSVIVAASCAMAVGPATALDLLGPGELAVVAGQMWCI
                     CGAEHKCICANYHDGCMAHPGGNCINDFINENSRVEHNKPYLTCRYGGVWSASCHDVS
                     NVKCGWAEVYENYDCLGAVTIQWHWATGCGYP"
BASE COUNT          299 a          594 c          636 g          303 t
ORIGIN      
        1 tcggcgggat catcatcgac gtggcccgca gcggccagac gctcttcccg gtgcctgact
       61 cgccctacat cacccgcgcg gggttcgaag acggcactca gctcacccgg tggatcgacg
      121 agatccacgc acaagggctg aaggcttacg ccggcgtcaa cctcctgcgt tggtggctgc
      181 tgttcagtga ggacccgaat ccattcgagc gcgtgcccga actcctcgag ctggactcga
      241 gcctgagctg ctccgacgag ctggacgcga agtacgcctc gatgtggcac ccgaaggtgc
      301 gcgcggggct ggtggccctc atgcgcgagc tggcgacacg gtaccccggc ttcgacgggc
      361 tctacctgca ggcccagttc tcccgcacgg cctacctcgg cttctcagat agcgcccgca
      421 tcgcgtacat ccgtcacgcc cacatcgacc cggtcgatct gcccctctac ggagtcgatg
      481 agttccagga caagtacctc ctggcctact tcgagtggcg gcccaagcag ctcaccgagc
      541 tggttcgcga ggtggtcggg gcctaccgcg agatcgccgg cgcggacgat cgagtgatcg
      601 ccatggcgac ggccaccggg ccggcgcggc ggccgcggca ccgcgcgacg ccgggcgatg
      661 actgggtgac ctggctgctg gagggcctgg cgagcgacct ggcgttcgag gcctcggtat
      721 ggctcgacgg ctggccgggg atgttccgcg cggggctgga caccttcggc cggctgcgga
      781 cgcctgctcc gacctacctg atcgtgtcgg gcgagcagcg cggggtgctg tcgccgatcg
      841 cggatgtcta ctcagcgctg agccagggcg acatatctgg cctgcccctg ctgctgtcac
      901 cctacgcacc ggcgcagctg gatggatcac tcaagctact gcggtcgctc cggtagccgg
      961 ctgatagtga ggcgaggtga tggcatggca accgatgatg cggaaacccg caccagtcgt
     1021 cgcgggttcc tgcggagctc cgcgggaatg agtctggcag ccttcgtggc actgccgacc
     1081 atggatgccg tggtctcggc cgtgaccgac cgtctgggcg aacgcgcgct cggccggcaa
     1141 ctcgcggaga cgctgcgcac gacggaggcg gcgcgttcgg agtccgtgtg tgaggcgtag
     1201 ccattccctg ggtcccctca gcggcccgcg ctggggcgac gcagtgggtt cggcgcgaga
     1261 atggacggag gtgtccagca gtgaggatct ccagcacact gttgtctgtg attgtggctg
     1321 cgtcgtgtgc catggccgtc gggccggcaa ccgcgctcga tctgctgggg ccgggggagc
     1381 tggctgtggt cgccggccag atgtggtgca tctgcggtgc tgagcacaag tgcatctgtg
     1441 ccaactacca cgatggctgc atggcccacc cgggcggcaa ctgcatcaac gacttcatca
     1501 acgagaacag cagagtggag cacaacaagc cttacttgac ctgcaggtac ggcggcgtct
     1561 ggagcgcctc atgccatgac gtttcgaacg tgaagtgcgg ctgggcagaa gtgtacgaga
     1621 actacgactg ccttggcgcc gtgacgatcc agtggcactg ggccaccggc tgcggctacc
     1681 cctagacggt cgcgcgatgg acccaaccgg agacgcgcgg caacctcctc aggcctgagt
     1741 agccgactgc ctcaggggag tacatcactc gcaggaggaa ctacccccac cgggaggggt
     1801 gcgcgatgga gacgaacacg aggggactcg gc
//
LOCUS       DAOSPL010001083         1827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_64255_length_1827_cov_3.034424, whole genome shotgun sequence.
ACCESSION   DAOSPL010001083 DAOSPL010000000
VERSION     DAOSPL010001083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64255_length_1827_cov_3.034424"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1667)
                     /locus_tag="PLQ54_16985"
     CDS             complement(<1..1667)
                     /locus_tag="PLQ54_16985"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOS95013.1"
                     /translation="MSLAEDLRESWMDPPSLYRGAPFWSWNERLVPDRLRRQIEAMAD
                     AGMGGFFMHSRYGLKTPYLSDEWFACIGACVEKARELGLKAYLYDEDRWPSGPAGGFV
                     TRAHPEFRVHCLCSGDPTGQGDLVKRLGSFRVELDGAGGLVSYEPCGDGEGLAFSVCT
                     PYPTGWENDGGYLDTMDSEAVDEYIRVTHEAYAARYADEFGETIPAIFTDEPNYGFWS
                     AGSAGEGARVQWTRQMEAEFAARRGYEITAHLPELVYPLSGGRFSRVRYDYHRTLTEL
                     FVEHFSAKIGRWCAEHGIALTGHYLLEGALDVQTRAVGACMPHYEHMQWPGIDILTDQ
                     ADELLTAKQCSSVADQLGLQRVLSELYGCTGWDWPLEGHKFVGDWQYAVGVNFRCQHL
                     SHYSLAGGAKRDYPASILYHSPWWPYYRVVEDYFGRLSLMLTQGRPVRDVLVVHPVES
                     AWGLYCSSAREPSPVMAEIESGLRAVMFTLTGQHYDWDFADESLLARHGQVVDGVVRV
                     GQMSYSLVVVPPCVTLRASTVEVLRGFLREGGKVLFVGRQPERIDAEA"
BASE COUNT          349 a          694 c          513 g          271 t
ORIGIN      
        1 gcctcggcat cgatgcgctc cggctgtcgg cccacgaaca gcaccttccc accctcacgc
       61 aggaaacccc ggagcacctc aaccgtgctc gcacggagcg tcacacacgg cggcaccacc
      121 acgagcgagt agctcatctg ccccacacgg accacgccat caaccacctg gccgtgcctc
      181 gccagaagcg actcgtccgc gaagtcccag tcatagtgct gcccagtcag agtgaacatg
      241 accgcccgca ggcccgactc aatctcagcc atgaccggcg acggctcgcg cgccgatgag
      301 caatacagac cccacgcgct ctcaacaggg tggacgacca gtacatcccg taccgggcgg
      361 ccctgggtca gcatcaggct gagccgcccg aagtagtcct cgaccacgcg gtagtagggc
      421 caccacggac tgtgatagag aatgctcgcc ggatagtccc gcttggcccc ccctgccagg
      481 ctgtagtgac tcaaatgctg gcagcggaaa ttcacaccga cagcatactg ccagtcaccc
      541 acgaacttgt ggccctccag tggccagtcc cagcccgtgc atccatacag ctcgctcagc
      601 acccgctgca gccccagctg gtcggccact gaactgcact gcttggccgt gagcagctca
      661 tcggcctgat ccgtcaggat gtcgatcccc ggccactgca tgtgctcgta gtgcggcatg
      721 caggccccca cggcccgcgt ctgcacgtcg agcgcgccct ccagcaggta atgccccgtg
      781 agcgcgatcc cgtgctcggc acaccaccgg ccaatcttcg ccgagaagtg ctccacgaac
      841 agctccgtca gggtccggtg gtagtcatag cggaccctcg aaaaccgccc gccactcagc
      901 ggatagacga gctccggcag atgggccgtg atctcatacc cccgccgcgc cgcgaactcc
      961 gcctccatct ggcgcgtcca ctgcacgcgg gccccctcgc ccgcgctgcc cgcggaccaa
     1021 aaaccatagt tgggctcgtc ggtgaagatg gccggaatcg tctcgccgaa ctcgtccgca
     1081 taccgggccg cgtaggcctc gtgcgtcacc cggatgtact cgtccaccgc ctccgagtcc
     1141 atcgtgtcga ggtagccacc gtcgttctcc cagccggtgg ggtagggggt acacacgctg
     1201 aaggctagtc cctcgccatc cccgcacggc tcataggaca ccagcccccc cgcaccatcc
     1261 agctccacgc ggaacgaccc cagccgcttc accagatcgc cctgccccgt cggatcgccc
     1321 gaacagaggc agtgcacgcg gaactccggg tgtgcccgtg tcacgaaacc tccagccggc
     1381 cccgaaggcc accggtcctc atcatagagg taggccttca gccccagctc acgcgccttc
     1441 tccacgcacg ctccgatgca agcgaaccac tcgtcagaga ggtagggagt cttcaaccca
     1501 tagcggctgt gcatgaagaa tccgcccatg cccgcatcgg ccatggcctc gatctgacgg
     1561 cgcagccggt ccggcaccag ccgctcattc cacgaccaaa acggcgcacc gcggtacaac
     1621 gaaggggggt ccatccacga ttcgcgaagg tcttcagcca gagacatcgg ctacactcct
     1681 ccaatgcggt gtgcgtctct cagggagaac cattatcgcc cactcggcgc ggtccgggag
     1741 ccacttcctg ccggcaaggg tgccatggcg gcaagcacgc gggcatggcc tggtggcccc
     1801 caatgggccc gggtcgaccg aaggccg
//
LOCUS       DAOSPL010001084         1827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_64269_length_1827_cov_2.385440, whole genome shotgun sequence.
ACCESSION   DAOSPL010001084 DAOSPL010000000
VERSION     DAOSPL010001084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64269_length_1827_cov_2.385440"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..>1827
                     /locus_tag="PLQ54_16990"
     CDS             61..>1827
                     /locus_tag="PLQ54_16990"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOS95014.1"
                     /translation="MGVLPIALTLLLPMPQVLAVDIPSLTWQERSDWTNVKTDVEPRA
                     VGDGQADDTAAIQAALDRAAASGTAYLPPGTYRITRTLAFTGPAVGCLVVGHGRDTVI
                     VWDGPEGGRMFRSNGVAYSRYVGLSWDGRGRAAVGFDHASELRFETEVRHEHEAFRNF
                     TGYGIRVGNEQNLASAEILYRNCLFEDCGTGLGFLTFNDYDNTIDRCEFVRCGTGVFD
                     NKGNFYARNCHFEGSAVADFSVGSEHGSSIRRCTSIGSRRFIEERGTIAPLTVQDCHV
                     AGWTDPQCAVLLAGSPVLMFDCSFDRPPSEASPVLETAGGQIFFTSNNRPGPAHQLVR
                     QSEGARLYEIPAGRLGGVVESADRRFLLEAVPSPGRVFDAVVDFGAKGDGQTDCTEAI
                     QQTIDAARTAGNGAVAYLPTGSYVVLKTLKVTGGDYTLSGSGFGCKLLWRGEPGQSIV
                     EVSDAEGVTLASMAIGHHDAGPMDHGADVLVTAPSGRPCRVVFDEVSVFGMYQKAPEA
                     HGIRFVGLPSGSVVDALHVQGNLRVIDSAAATLLFRTSYEGTVTLEGTAGGRGGLVGF
                     LTRLATLSKPTLHVRDNLSIVMS"
BASE COUNT          282 a          594 c          632 g          319 t
ORIGIN      
        1 ccgtgcggcg ctggcgccga actgcggcgc acgtcattcg ccgggggtgc cgtcatgcga
       61 gtgggtgttc tcccaatcgc tctcacgctt ctcctgccca tgcctcaggt gctggccgtc
      121 gacatcccct cgctcacgtg gcaggagcgc tccgactgga ccaacgtgaa gactgatgtc
      181 gagccgcggg ccgtgggcga cgggcaggcg gatgacacgg cggcgataca ggccgcgctg
      241 gaccgggcgg cggccagcgg caccgcctac ctgccgccgg gaacgtaccg catcacccgt
      301 acgcttgctt tcaccggccc agccgtaggg tgccttgtgg tcgggcatgg gcgcgatacc
      361 gtcatcgtct gggacggccc cgagggcggt cgcatgttcc gcagcaatgg cgtggcctac
      421 agccgctatg tgggcctgag ttgggatggg cgtgggaggg cagccgtcgg gttcgaccat
      481 gcatcggagc tgcggttcga gacggaggtc cggcacgagc acgaggcctt ccgcaacttc
      541 acgggctacg ggatccgcgt cggaaacgag cagaatctcg cctcggccga gatcctctac
      601 cgcaactgcc tgttcgagga ctgcggcacg gggctcgggt tcctcacttt caacgactac
      661 gacaacacca tcgaccgctg cgagttcgtc cgttgcggca caggggtctt cgacaacaag
      721 ggcaacttct acgcccgcaa ctgccacttc gagggcagcg cggtggccga tttcagtgtc
      781 ggcagcgagc acggcagctc gatccgccgg tgtacgtcca ttggttcgcg gcgtttcatc
      841 gaggagcgcg gcaccatcgc tccattgacg gtgcaggact gccatgtggc gggttggacc
      901 gacccccagt gcgccgtgct gctcgccgga agcccggttc tcatgttcga ctgttcgttc
      961 gatcgccccc catcggaagc gtctcctgtc ctcgagacgg ccggcgggca gatcttcttc
     1021 accagcaaca accgccccgg tcccgcacac cagctcgtgc gccagtccga aggtgcgcga
     1081 ctgtacgaga tcccggctgg caggctcggc ggcgtggtgg agtcggccga ccggcgcttc
     1141 ctgctggagg cggtgccgtc gcccggccgc gtgttcgacg ctgtggtcga cttcggagcc
     1201 aagggggacg gtcagacgga ttgcacggaa gcgatccagc agaccatcga tgcggcgagg
     1261 acggccggga atggggcggt cgcctatctc ccgacgggtt cctatgtggt gttgaagacg
     1321 ctcaaggtca cgggcggtga ctacacgctc agtggtagcg gcttcgggtg caagctgctc
     1381 tggcgcggcg agcccgggca gtccatcgtc gaggtgagcg acgccgaggg cgtgaccctc
     1441 gccagcatgg cgatcgggca ccatgatgcg gggcccatgg accacggcgc tgacgtgctg
     1501 gtgacggcac cgtcgggccg accatgccga gtggtgttcg acgaagtgtc tgtcttcggc
     1561 atgtaccaga aggcaccgga ggcgcatggc attcgcttcg ttgggctacc ttccggcagc
     1621 gtggtggatg cgctacacgt ccagggcaac ctacgtgtca tcgacagcgc cgcggcgacg
     1681 ctcctgtttc gcacgtccta tgagggcacg gtcacgctgg agggcacggc aggcggccgc
     1741 ggcggcctcg tgggcttcct gacgcgtctg gccaccctct cgaagcccac actgcacgtt
     1801 cgcgacaacc tgtcgatcgt tatgagc
//
LOCUS       DAOSPL010001085         1824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_64414_length_1824_cov_2.581119, whole genome shotgun sequence.
ACCESSION   DAOSPL010001085 DAOSPL010000000
VERSION     DAOSPL010001085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64414_length_1824_cov_2.581119"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..>1824)
                     /locus_tag="PLQ54_16995"
     CDS             complement(120..>1824)
                     /locus_tag="PLQ54_16995"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95015.1"
                     /translation="GLIVKRRAQKSIRSYFLGERRLPWWALAMSGSSSYFDITGTMWI
                     VSLFFVMGFRGMWVQWIWGFPITVFYLAYMGPWIRRSGVMTGAEWMETRFGSGKAGEM
                     ARLAYTVYAILTITAFLAYGAEGMGKFGSVYLGMDEHTCSALIIGVTGLYVVVGGFHG
                     VVLVEIFQTVVLSAGALLIAYLGFTRVSWEAVAAARPADWAGILPAWRFDYLRDTEYY
                     CFGALLLVWLTKGILLDLSGPEQLYDFQRFLAARSPREASKLGALWGAVHTIRWPMAM
                     AIAAMAIVGLSGVTDPEKVLPQVINDVLPVGLRGLAIAALLSGFLATFNSTVNGGASY
                     LVKDVFHKYLRPQASERTLIVASYVSSAMLIVLGILIGTQAKSINQMFTWIMGTLGAG
                     VLLPNVLRWYWWRLNGWGYAAGALSGMALSLVQALVPSLNAQPLYVTFPAIAGAVLAV
                     SVAVSLRCPPTDSETLNRFYRTVQPWGCWRPVAEAVRAADPAFARRAPVSRDVVNVLL
                     GLPCLLAMYTFPIYLIVHRFAEAAILFGVMATLCVALYFTWYRHLSDFEHEELTEEAA
                     A"
BASE COUNT          383 a          589 c          590 g          262 t
ORIGIN      
        1 gcctcggtag tgccggccct cgaatgaggg cggttccgct gcgttgccca tgaggactcc
       61 ccccagtatg gtcccacaac cgacgagtgc cacaaccagt cgcctgcgat gagaggtgct
      121 catgctgcgg cctcctcagt aagctcctca tgctcgaagt cagataggtg acggtaccat
      181 gtgaagtaga gagcaacaca cagagtggcc atcacgccga acaggatggc ggcttcggcg
      241 aaacgatgga caatgaggta gatggggaac gtgtacatgg cgagcagaca gggcaggccg
      301 agcagaacgt tcaccacgtc gcgagagacg ggcgcccggc gggcaaaggc cgggtcagca
      361 gcccgcacgg cttcagccac cggccgccag cacccccatg gctgcaccgt gcggtagaac
      421 cggttgaggg tctcggaatc ggtgggggga caccttaggc tgaccgcaac actgactgcc
      481 agcaccgccc cggcgatggc agggaacgtg acatacagcg gctgcgcgtt cagcgagggc
      541 accagggcct gaaccagtga cagggccatt cccgagaggg cgccggcggc gtagccccag
      601 ccgttgagcc gccaccagta ccagcgcagt acgtttggga gcaggactcc ggccccgagc
      661 gtccccatga tccaggtgaa catctggttg atcgacttgg cctgggtgcc gatgagaatg
      721 cccagaacga tcagcattgc cgaggacaca tagcttgcca cgatcagcgt gcgctcggag
      781 gcctgggggc ggaggtactt gtggaacaca tccttgacca ggtatgaagc tccgccgttc
      841 acggtggagt tgaacgtggc caggaagccc gacagcaacg cggcgatggc gaggccgcgg
      901 agccccacgg ggagcacatc gttgatgacc tgcgggagca ctttctcggg atcggtgaca
      961 ccgctgagcc cgacgatggc catcgccgcg atcgccatgg ccatgggcca tcggatggtg
     1021 tgaacggcgc cccacagggc cccgagcttc gaggcctccc gcgggctgcg tgccgcgagg
     1081 aaccgctgga agtcatacaa ctgctcgggc ccactcaggt ccagcaggat ccccttggtc
     1141 agccacacga gaaggagagc gccgaagcag tagtactcgg tgtcgcgaag gtagtcgaac
     1201 ctccaggcgg gcagaatccc cgcccagtcc gccgggcggg ccgctgcaac ggcctcccac
     1261 gagacccggg tgaacccaag gtaggcgatg agcagggcgc cggcacttag cacgaccgtc
     1321 tggaagatct ccaccagcac cacgccgtga aagccgccga cgaccacgta gagcccggtg
     1381 accccaatga tgagcgccga gcaggtgtgt tcatccatgc ccaggtagac ggacccgaac
     1441 ttgcccatcc cctcggcacc gtaagcaagg aatgccgtga tcgtgaggat ggcatagacc
     1501 gtgtaggcca gccgggccat ttcgcccgcc ttgccactgc cgaaccgggt ttccatccac
     1561 tcggcccccg tcatgacgcc cgagcggcgg atccacgggc ccatgtaggc gaggtagaac
     1621 accgtgatgg ggaagcccca gatccactgc acccacatgc cccgaaagcc catcacgaag
     1681 aataggctga cgatccacat ggttcccgtg atgtcgaagt aagacgagct cccggacata
     1741 gccagcgccc accacggcag acgcctctcg ccgaggaagt atgagcggat gctcttctgg
     1801 gcccgccgtt tcacgatgag cccg
//
LOCUS       DAOSPL010001086         1823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_64445_length_1823_cov_3.255090, whole genome shotgun sequence.
ACCESSION   DAOSPL010001086 DAOSPL010000000
VERSION     DAOSPL010001086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64445_length_1823_cov_3.255090"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="PLQ54_17000"
     CDS             complement(<1..1203)
                     /locus_tag="PLQ54_17000"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HOS95016.1"
                     /translation="MLVFTQWVSLMAAVFGAGIEPPPTLAGAGFLTVPGERWPLTWHD
                     EFEGDALDTSKWTIGLPWPGDDGEGRHHNTQYASYIMDDDVAVRDGALHLTTQRRDVT
                     AANGQAYHFTEGLITTHSTFRSLFGYFEIRAALPTDAGPGTWPAFWMLADGWPPEMDV
                     IEYWGSTNRIHQGTATAKPDGGQRWDSYHTSDVSLTGWHTFGLEWGPGYQIYHIDGHI
                     TNAIYGEHVPAVPHYLLLNSGIETARPPLPGTAFPNDFAVDYVRVYERPDVPALLNGG
                     FESPALAPWGRWNSAAAVDYNQRSGRRALRVDGREGDDPASSAQQTVYGLQPTTTYRL
                     AAWARVSGAAVARLGVKDHGGDETRSDGLALTDYGELSLKFTTGTNTTRATVYCWAEG
                     PGSAFFDDV"
     gene            1432..>1823
                     /locus_tag="PLQ54_17005"
     CDS             1432..>1823
                     /locus_tag="PLQ54_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95017.1"
                     /translation="MSFAMTEIGGVPISRLMIGTNTFHGFSHFSGSRDRWLRQYFTPE
                     RIYEVLEYCVGQGLNATIGMQRKDYADLLDEIHRNTGTRIHYIATPGGGTLEELKKGI
                     REAADLGCEFCWPHTSWTDVRVLPSEGR"
BASE COUNT          291 a          579 c          632 g          321 t
ORIGIN      
        1 cacgtcatcg aagaaagcgg atccgggccc ttcggcccag cagtagacgg tggccctggt
       61 ggtgttggtg cccgtcgtga acttcagtga gagttcgccg tagtcggtca acgcgagccc
      121 gtccgagcgt gtttcgtcgc caccatggtc tttgacgccc agccgcgcga ccgcggcgcc
      181 gctcactcgc gcccaggcgg cgaggcggta tgtcgtcgtt ggctgcaggc cgtacacggt
      241 ctgttgcgcc gatgacgccg ggtcgtcacc ttcccgccca tccacgcgga gcgcgcggcg
      301 gccggagcgc tggttgtagt cgacggcggc cgccgagttc catcggcccc aaggcgccag
      361 ggccgggctc tcgaatccgc cgttcagcaa cgccggcaca tccggtcttt cgtagacgcg
      421 cacgtagtcg acggcgaagt cgttcgggaa cgccgtgccc ggaagcgggg gccgcgcggt
      481 ctcgatgccc gagttcagca gtagatagtg gggtaccgct ggaacgtgct cgccatagat
      541 ggcattcgtg atgtggccat cgatgtgata gatctgatag cccggccccc actcgaggcc
      601 gaaggtgtgc cagccggtca gcgagacatc actggtgtgg tagctgtccc accgctggcc
      661 gccatcgggc ttggctgtcg cggtgccctg atggatccgg ttcgtgctgc cccagtattc
      721 gatgacatcc atctcgggag gccaaccgtc cgccaacatc cagaatgcgg gccaagtgcc
      781 cgggccggcg tctgtcggga gtgcggcgcg gatttcgaag tatccaaaga ggctccggaa
      841 agtcgagtgg gtagtgatga ggccttcggt gaagtgatag gcctggccgt tggccgcagt
      901 cacgtcgcgc cgctgcgtgg tcaggtggag ggctccgtcg cggacggcta cgtcgtcatc
      961 catgatgtag ctggcgtact gggtgttgtg gtggcgcccc tcgccatcgt cgcccggcca
     1021 gggaaggccg atggtccact tggaagtgtc gagtgcatcg ccctcgaact cgtcgtgcca
     1081 ggtgagaggc caccgctctc cgggcacggt gaggaagccc gcgccggcca gagttggcgg
     1141 cggttcgata ccggcgccga aaacggccgc catcagggaa acccattggg tgaacacgag
     1201 catcccctcc cccgggtcgc gacgacgctc cgagaacgcc tgcggcgagc tgcgtttcga
     1261 gcggtgcccg atgagggcct ccaatgctca gcggcacctg ccgttggccc ggccccgtca
     1321 cccgcctcgc gcggccgggg cgatcgagag ccgtccggta ggacccgcgc acgatcggac
     1381 gaatccatag agctgttgtg tcgctgcgcg tgaccctagg aggaaggccg catgtccttc
     1441 gcgatgactg agatcggcgg tgtgccgatc tcgcgcttga tgatcgggac gaacaccttc
     1501 catggctttt cgcacttctc tggctcgcgc gatcgttggc tccgccagta cttcacgccg
     1561 gagcggatct atgaggtgct cgagtactgc gttggccagg ggctgaatgc cacgatcggc
     1621 atgcagcgca aggactacgc cgacctgctg gacgagatcc accggaacac gggcaccagg
     1681 atccactaca tcgcgacgcc gggcggcggg acgctcgagg agctgaagaa gggcatccgc
     1741 gaggcggccg atctcggttg cgagttctgc tggccgcaca cgtcatggac cgatgtgcgt
     1801 gtgctgccca gcgagggccg cat
//
LOCUS       DAOSPL010001087         1821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_64521_length_1821_cov_3.817101, whole genome shotgun sequence.
ACCESSION   DAOSPL010001087 DAOSPL010000000
VERSION     DAOSPL010001087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64521_length_1821_cov_3.817101"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1821)
                     /locus_tag="PLQ54_17010"
     CDS             complement(<1..>1821)
                     /locus_tag="PLQ54_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95018.1"
                     /translation="AALANLALSAAQRHPLSTGMWNRVYWADPTAPSPTTRDREHQWL
                     ADLQQRAQSVLRQTGAARASATANADNLAYLEMTALKLSTLAGRRLAFLQAADLYAEA
                     ANAASADAAVPRLMTARRLLVAALADIRAIGDRYARLWLAEARPYWLDVQTPKYAAMG
                     RLIKDRVDRLDAAAAGLRDGRPLPPAAEVGLAIAFPAERGVRPTVSDQPLPAGSNDWF
                     DPAWHYRIGVRIEARSLPRTDLPVEIELDLAPRLPGHTLDPGSIRVVSCDASGHHEHE
                     IPSQLMTDGLRVPHIAFIAAGRIETGQSRHFRIYFDTVANGSKTHPTYPGVSLSRADG
                     GYAIANERVRWLVLPEGAHVYEWHVNALGGVDITDPGRAGYSGFLDVNTSRTASFALT
                     PLAEGPVVARLRCESPDGAEKVLSFYAGAGWVEADISPETTYCWNFDPATLMASDSPT
                     PGTARFSTGYSAPVGRSGVLGQVRAAGAVWGCKYRPDGLTLGMVTPGRPTTHVIGPGG
                     GWGGAGIEGGAGASHFVAVGDVLLDPPAVLSSLAATLDYTNPPAITLGRVEQKPEDAA
                     SGGRAARAVPFAAPVRCDAARCRCERAGGSGRIQRCCGLA"
BASE COUNT          262 a          627 c          670 g          262 t
ORIGIN      
        1 cggcgagccc gcagcagcgc tgtattctac ctgatccccc ggcgcgttcg catctgcacc
       61 gggccgcgtc gcagcggacc ggtgcggcga acggcacggc acgcgccgcc cgccctccgc
      121 tcgcggcgtc ctcaggcttc tgctccaccc ggccgagtgt aatggccggc gggttggtgt
      181 agtccagtgt ggccgccagg gaactgagca cggcgggagg gtcaaggaga acgtcgccca
      241 ctgcgacgaa gtggctggcg cccgcgccgc cctcgatgcc ggcgccaccc cagccgcccc
      301 ccggcccgat gacgtgggtc gtcggccggc ccggggtgac catgcccaac gtcagcccgt
      361 cgggacggta cttgcagccc cagacagccc cggcggccct cacctgcccg agtaccccgg
      421 agcgcccgac cggcgcgctg tacccggtcg agaagcgtgc cgtgccgggc gtcgggctgt
      481 ctgaagccat cagcgtcgcc gggtcgaagt tccagcagta ggtggtctcg ggcgagatat
      541 cggcctcgac ccatcccgcg ccggcataga agctcagcac cttctccgcg ccgtccggcg
      601 actcgcaccg gaggcgagcg acgacaggcc cctcggccag cggagtgagc gcgaaggacg
      661 cggtgcggct cgtgttcacg tccaggaagc cagagtaccc cgcccggccc ggatcggtga
      721 tgtcgacgcc gcccaaggca ttcacgtgcc attcgtatac gtgggcaccc tcgggcagca
      781 cgagccagcg gacgcgttcg ttcgcgatgg cgtacccgcc gtcggcccgc gatagggaca
      841 ctccgggata cgtcgggtgg gtcttggagc cgttcgccac cgtgtcgaag tagatgcgga
      901 agtggcggct ctgccccgtc tcgattcgcc cggccgcgat gaacgcgatg tgcggcactc
      961 tcagcccgtc ggtcatgagc tgagagggga tctcgtgctc gtggtggccc gaagcatcgc
     1021 acgacacgac ccggatggag ccgggatcga gtgtgtggcc cgggagccgt ggggccaggt
     1081 ccagctcgat ctccaccggc aagtcagtcc gcggcaggga acgtgcctcg atgcgtactc
     1141 cgatgcggta gtgccacgcg gggtcgaacc agtcgttgga cccagcgggc agcggctgat
     1201 cgctgacggt aggccggacg ccgcgctcgg cggggaaggc gattgcgagg ccgacctcgg
     1261 ctgccggggg caagggcctg ccgtctcgca ggccggctgc cgccgcatcg agcctatcga
     1321 cgcggtcctt gatgagccgg cccatggcgg cgtacttggg ggtctgaaca tccagccagt
     1381 agggccgcgc ctcggccagc cacagcctcg cgtagcggtc gccgatggca cggatatcgg
     1441 ccagggcggc caccagcagc ctccgggccg tcatcaggcg cggtaccgcc gcgtccgcgc
     1501 tggcggcgtt cgcggcctcg gcgtagaggt cagcggcctg caggaaagcg agccgtcggc
     1561 ccgccagggt cgagagcttc agagcagtca tctcgaggta ggccaggttg tccgcgttgg
     1621 ccgtggcact ggcgcgcgca gccccggtct ggcggaggac actctgggcc cgctgttgca
     1681 ggtcggcaag ccactggtgc tcgcggtcgc gagtggtggg tgatggagcc gttgggtcgg
     1741 cccagtagac acggttccac atgccggtcg acagcggatg gcgctgggcg gccgagagcg
     1801 ccaggttggc gagcgcggcc g
//
LOCUS       DAOSPL010001088         1819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_64621_length_1819_cov_4.374717, whole genome shotgun sequence.
ACCESSION   DAOSPL010001088 DAOSPL010000000
VERSION     DAOSPL010001088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64621_length_1819_cov_4.374717"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1602)
                     /locus_tag="PLQ54_17015"
     CDS             complement(37..1602)
                     /locus_tag="PLQ54_17015"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOS95019.1"
                     /translation="MFCTGCGLQLPEQGRFCPKCGRPVAGQAPPPAVPPVIPAPVPVP
                     APVPVPTPSVVAPQPRKSRRWLVLALVPVVLIGLVGGGFALWSKLRSPGDDGFYRSPT
                     ITSATRAPTKAWSIQMPSIAKGGPWTKDGRTSVRELGDLLLVSARGSGSNETGQITVV
                     DPASGKIRWSSKETDDPGYCDRLDDLLLCGVPENTDSRQHIIAYDINNGSVRFRTESE
                     PNLGWEVADALYTYSSVGTDYKAPTKSTITKYSPDGKKVWTNTDLYKLFNADAYGMRL
                     AAGRVAMLNATMKDGKAFVLDASTGLPAPDRPVGIVVSAPVDGYWSAEPASNDAGLIV
                     RGRTIGEGQGDVAGALWYTLDGAGQPVVLAHVGRDDARTTSVYSFKGHESTLRFRSDK
                     TINGFCGERAMAFGNEGMIGLDSRTGAQSWSEPILGAWDWRCTASGDALMAAIVYLLP
                     VGEGQPSTNSGSRLVGVNTKSGNQLWTAPLENLRVNPTDRLETTFTTAGFAVLSVSNT
                     DTMSATLHFLKAN"
BASE COUNT          337 a          588 c          552 g          342 t
ORIGIN      
        1 ccgggccgac tcacggcaat gggtatgccg tgtggctcag ttggccttga ggaagtgcag
       61 cgtggcgctc atggtgtccg tgttcgagac ggacagcacc gcgaaacctg ccgtcgtgaa
      121 cgtcgtctcg agtcgatcgg tcggattgac ccgtaggttc tccagcggtg ctgtccacag
      181 ctgattaccg gacttcgtgt tcacgccgac cagccgtgag ccactgttcg ttgatggctg
      241 gccctcgcca actgggagca ggtagacaat cgcagccatc aacgcatccc cggacgcggt
      301 gcatctccaa tcccaagccc ccaagatggg ctcgctccat gactgcgcac cggttctgga
      361 gtcgagtcca atcattcctt cgttcccgaa ggccatcgcc cgctccccgc agaagccatt
      421 gatcgtcttg tcgctacgga agcgcagcgt gctctcatgg cccttgaagg aatagaccga
      481 ggtggttctg gcatcatcgc gacccacatg agcgagaacg accggttgac cagcgccgtc
      541 aagggtgtac caaagggctc cagcaacatc gccttgtccc tcaccgatgg tcctaccccg
      601 aacgatgagt ccggcgtcgt tacttgccgg ttcggccgac caatagccgt ccaccggggc
      661 ggacaccacg atgccaacag gccgatcggg cgcgggcagt ccagtgctcg cgtcgagaac
      721 gaaggccttg ccgtctttca tcgtcgcgtt gagcatggct acgcgtcctg cggcaagacg
      781 cattccatag gcatcagcat tgaacagctt gtagagatcc gtgttcgtcc agaccttctt
      841 gccgtccgga gagtacttgg tgatcgtcga cttggtgggc gccttgtagt cggtccctac
      901 ggaactgtag gtatacagcg catccgcaac ctcccaacca aggttcggtt ccgactcggt
      961 ccggaatcgc acacttccgt tgttgatgtc gtaggcaatg atgtgctgcc gtgaatcagt
     1021 gttctccggg acgccgcaca acaagaggtc gtcgaggcga tcgcaatatc ccggatcatc
     1081 ggtctccttg gatgaccaac ggatctttcc actggccgga tccacaacag tgatctggcc
     1141 cgtctcgttg ctcccactgc ccctggcact cacgaggaga aggtcgccga gttcccggac
     1201 agaggtacgg ccatccttgg tccatggtcc acccttggcg atcgagggca tctggatcga
     1261 ccaggccttg gtcggggccc tcgtggccga cgtgatggtg ggggaccgat agaagccgtc
     1321 atctccgggg gaccgaagct tcgaccagag ggcgaacccg ccgccgacga ggccgatgag
     1381 caccacgggg acgagagcca gaaccagcca gcgccgcgac ttcctgggct gtggagcaac
     1441 gaccgaaggc gtgggcacgg gcactggcgc cggcacgggc acgggtgccg gtatcaccgg
     1501 gggcaccgct ggtggcgggg cctgacccgc cacgggacga ccgcatttgg gacagaagcg
     1561 cccctgctcg ggcaactgga gcccgcaccc agtacagaac atgccgttcc tccaccttct
     1621 gatgtccact cgagtcttcc gacgattcgc cctcggcgtc agtcgccaga agaggggaca
     1681 cccgccgtcc cggaagaccc cctcgagacc aaagcaaaag gtatgccggc cgctcacggt
     1741 gagcggccgg cataccttgt ggttttgcct tgctgtcctc aggcgtcgac ggtttccttc
     1801 tcgccctcgg aaccctcga
//
LOCUS       DAOSPL010001089         1819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_64642_length_1819_cov_2.993764, whole genome shotgun sequence.
ACCESSION   DAOSPL010001089 DAOSPL010000000
VERSION     DAOSPL010001089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64642_length_1819_cov_2.993764"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..506
                     /locus_tag="PLQ54_17020"
     CDS             180..506
                     /locus_tag="PLQ54_17020"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HOS95020.1"
                     /translation="MRKNRGFTLIEMMIVILVIAVLATIVGVAVRNAGERSKQAALTA
                     HLTTLNNAIQLYNNDMGSFPSDLTALTSTTNSDPNYNGPYLRSVPAHPYGGSWSYDSS
                     TGVVTP"
     gene            complement(585..1454)
                     /gene="rfbD"
                     /locus_tag="PLQ54_17025"
     CDS             complement(585..1454)
                     /gene="rfbD"
                     /locus_tag="PLQ54_17025"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOS95021.1"
                     /translation="MHVLVTGANGLLGSALVSQLAPHHDVLGLDLPELDITDRASVER
                     AFECAAPDVVIHCAAYTDVDRAESEPDLALLVNGQGAGLVAQCCEARGSHLIHISTDY
                     VFSGEAGHAPFLPTDRPAPINAYGHSKLAGEVAVREHCPAATIVRTCGLYGLRGRSFV
                     AAILSRALAGEPLKVVDDQTVGAPTYAVDLAQALATLASLRPGGIVHLVNAGVCTWYE
                     FAVEVLRAGGLAEHPIEPVPTDQFARPARRPAFSALASGAAHSAGLALRPYAEALHDF
                     IPTWLAEWTRRAR"
     gene            complement(1568..>1819)
                     /locus_tag="PLQ54_17030"
     CDS             complement(1568..>1819)
                     /locus_tag="PLQ54_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HOS95022.1"
                     /translation="LKGNVCVLAHKGKHCQGPITIAGCGAICPSHGGHCVGCRGYLAH
                     PERDAHMEILQEHGLTVDEVRAQYSLFNTYSEMAREAAS"
BASE COUNT          334 a          629 c          555 g          301 t
ORIGIN      
        1 ctcccgacta ctgccatctg ccatctggtg tctgccccaa gattgtggcg tgattcgcgt
       61 catccggtgc cacgacgcgc gtccgctatg gcaaaacatc actgatgatg ctcggcatca
      121 cgtctgtgac aacatccagc cccctgggct gtggtcgagt ggcgatggag gtgtctttct
      181 tgaggaagaa caggggtttc acgctgattg agatgatgat cgtgatcctg gtgatcgcgg
      241 tcctggccac catcgtgggt gtcgcggtcc gtaatgcggg ggagcgctcc aagcaggcgg
      301 ctctcactgc tcacctgacg accctcaaca acgccatcca gctctacaac aatgacatgg
      361 ggtcattccc atccgatctg acggcgctga catcgacgac caacagcgac ccgaactaca
      421 acggcccgta tctccggtcg gtcccagccc atccctacgg tggaagctgg tcctacgact
      481 cctcgaccgg tgtcgtcact ccatagctca cgcataccac gacgctccca gcgacacatc
      541 gggcgccccg ctcccagggc gcccgaccgc ttgtaccgcc ccggtcagcg agctcttcgg
      601 gtccactccg caagccaagt ggggatgaag tcgtgcagtg cttctgcgta cggccggagt
      661 gcaaggcccg ccgagtgagc cgccccactg gccagtgccg agaaagcggg ccgtcgggcc
      721 gggcgggcga actggtcggt gggcaccggc tcaatcgggt gttccgcgag cccgccggcc
      781 cgcaggacct cgaccgcaaa ctcataccac gtgcacaccc ccgcgttgac gaggtgaacg
      841 atgccccccg gccgcagcga tgcaagtgtg gccaaggctt gggccagatc gaccgcatag
      901 gtcggggcgc cgaccgtctg gtcgtcaacc accttcagcg gttcgcccgc caacgcgcga
      961 gagaggatcg ccgccacgaa gctccggccc cggagcccat atagcccgca tgtgcgcacg
     1021 atggtggccg cagggcagtg ctcacgcacc gcgacctcgc ccgcgagctt cgagtggcca
     1081 taggcgttga tcggcgccgg acggtccgtg ggcagaaagg gggcgtgtcc cgcctcacct
     1141 gagaaaacgt agtctgtgct gatgtgtatg aggtgtgacc cacgcgcctc gcagcactga
     1201 gcgaccaacc cagccccttg cccattcacc agcagggcaa gatccggttc ggactcggcc
     1261 cggtcgacgt ccgtgtaagc ggcacagtgg atcaccacat ccggcgctgc acactcgaac
     1321 gcccgttcaa cggaggcccg gtccgtgatg tccagctcgg ggaggtcgag acccaggaca
     1381 tcgtgatggg gagccaactg actcacgaga gcgctaccga ggagcccatt ggccccggtc
     1441 acaagcacgt gcatgaatac ggccagccat tccagtggag gccacgagcc gcgagccagg
     1501 tcggcttcgg cgctcgtcct ccacccacgc ccgaacaacc atgcgggcaa cccggcccag
     1561 tcgccggtca ggacgccgct tcccgagcca tctccgagta cgtgttgaac aacgagtact
     1621 gcgcgcgcac ctcgtccacc gtcagtccgt gttcctggag gatctccatg tgtgcatcgc
     1681 gctcggggtg agccagatag ccgcggcacc ccacgcaatg ccctccgtgc gatggacaga
     1741 tggctccgca gcccgcgatg gtgatggggc cctggcagtg cttccccttg tgtgccagaa
     1801 cacacacatt gcccttcag
//
LOCUS       DAOSPL010001090         1819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_64647_length_1819_cov_2.812925, whole genome shotgun sequence.
ACCESSION   DAOSPL010001090 DAOSPL010000000
VERSION     DAOSPL010001090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64647_length_1819_cov_2.812925"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1818)
                     /locus_tag="PLQ54_17035"
     CDS             complement(130..1818)
                     /locus_tag="PLQ54_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOS95023.1"
                     /translation="MLQAAQAAGIRIPTLCYHPDLPAQGACRVCLVEIEGERVLQPSC
                     TFKVREGMCVHTRSPRVRKARRTVVELLLSDHPSDCTACARNGTCELQSLAEELGIRE
                     VRVKAEPPGHEVDDSSFSVIRDPDKCVLCRRCIETCTVIQSVSGLRIKGRGYDSVVGA
                     AFDLPLGSALCVNCGQCINRCPTGALTEKSHIGAVWAALEDPDTFTVVQTAPAVRVAL
                     GEALGMDPGKIVTGKMVAALRALGFDRVFDTDFTADLTIMEEGYELIHRVTTGGVLPQ
                     LTSCSPGWIKFIEHFYPDLLPNVSSCKSPQQMFGALAKTYFAQEAGVDPAKMRVVSIM
                     PCTAKKFEAARPEMNDSGYQDVDYVLTTRELAQMIREIGIDFETLEESGFDDPLGQST
                     GAAVIFGATGGVMEAALRTAYEVITNEKLEDIDIHAVRGMQGVKEATVQVGNLELKAA
                     VAHGLANARQLLDRVARGEGGYHFIEIMACPGGCLGGGGQPIPTSMEIRKSRAAAIYQ
                     ADRDLPIRRSHENPSIQAIYEKFLGKPLSEKSHHLLHTKYTPRTRRANQVATRE"
BASE COUNT          315 a          601 c          571 g          332 t
ORIGIN      
        1 atggtccttc ctatcaggcc cctccgcaag tccaggcagg gcgctcggcg cgaccttcgc
       61 gagacccagt cgcccaacta agagcccaat gttagcgccg atggggcaga gccccgcccc
      121 tccacttcgt cactcgcggg tcgccacctg gtttgcacgc cgcgttcgcg gtgtgtactt
      181 ggtgtgcaac aggtggtgcg acttctcgct tagaggctta cccaggaact tctcgtaaat
      241 cgcctggatc gatgggttct catgcgaccg ccggatgggt agatcgcgat cggcctggta
      301 aatggccgcc gcccgggact tgcggatctc catcgatgtc ggtatgggct gcccgccgcc
      361 accgagacac ccgcccggac aggccatgat ctcgatgaaa tggtagcctc cctcgccccg
      421 ggccacccgg tccaggagct gtctggcatt ggccagtccg tgcgccaccg cggccttcag
      481 ctcaaggttg cccacctgga cggtggcctc cttgacgccc tgcataccac ggacggcgtg
      541 gatatcgatg tcctcaagct tctcgttggt gatgacctca taggctgtac gcaaggccgc
      601 ttccattacc ccaccggtag caccgaagat gacggcggcg cccgtagact ggccgagagg
      661 gtcgtcgaag ccgctctcct caagggtttc gaagtcgatc ccgatctctc ggatcatctg
      721 cgccagctca cgggtcgtca ggacgtagtc cacgtcttga tagcccgagt cgttcatctc
      781 gggccgcgcc gcctcaaact tcttcgccgt gcacggcatg atggacacca cgcgcatctt
      841 ggccggatcc acaccagctt cctgggcgaa gtacgtcttg gccaaggccc cgaacatctg
      901 ctgaggagat ttgcatgacg acacattggg cagcaggtcc gggtagaagt gctcgatgaa
      961 cttgatccat cccggactgc acgacgtgag ctgagggagc accccgccgg tcgtgacccg
     1021 atggatgagc tcgtagccct cttccatgat cgtcaggtcg gccgtgaagt ccgtgtcgaa
     1081 cacccggtcg aaacccagcg cgcgcagggc cgcgaccatc ttgcctgtga cgatcttccc
     1141 gggatccatc ccaagcgcct cgccgagtgc cacgcgcacg gccggtgcgg tttggacgac
     1201 ggtgaaggtg tccgggtcct ccagggcggc ccagacggcg ccgatgtggc tcttctcggt
     1261 cagcgcaccg gtaggacacc ggttgatgca ttgaccgcag ttgacgcaga gggcgctacc
     1321 aagcggcaag tcgaaggcag ctccgacgac gctgtcgtac cctcggccct tgatacggag
     1381 gccggagacg gactgaatca ccgtgcatgt ctcgatgcat cggcggcaga gcacgcactt
     1441 gtcggggtcg cgaatgaccg agaagctcga gtcgtccacc tcgtggccgg gcggttcggc
     1501 cttgacacgc acctcgcgta tgccgagttc ctcggccaag ctctgcagct cgcaagtgcc
     1561 attccgggcg caggcggtgc agtccgatgg gtggtcggag agcagcaatt cgaccactgt
     1621 acggcgcgcc ttgcgcaccc gaggcgaccg agtgtggacg cacattccct cgcggacctt
     1681 gaaggtacag gatggctgga gcacgcgttc gccttcgatc tcgaccaagc acacgcggca
     1741 cgcgccctgt gcgggcagat ccgggtggta gcacagggtc gggatgcgga tacccgccgc
     1801 ctgggccgcc tgcagcacg
//
LOCUS       DAOSPL010001091         1818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_64698_length_1818_cov_2.926829, whole genome shotgun sequence.
ACCESSION   DAOSPL010001091 DAOSPL010000000
VERSION     DAOSPL010001091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64698_length_1818_cov_2.926829"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PLQ54_17040"
     CDS             <1..527
                     /locus_tag="PLQ54_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95024.1"
                     /translation="GGWVPVREADAAAIAVTEAGEWAIRTAAGESVSWRQTLPGVGAG
                     ITIEAAARVTAVGDAGEGLGLRLAVPRAGGQVARYGISVTRTGVYWYAGVWTPLIEWV
                     DHHTATHRFRLAIRPDGGAQVYRDGELLGVRMPVIEPGESPGPLAEWGIGAADACEVA
                     VAQVAVDTSGAYAP"
     gene            626..1747
                     /locus_tag="PLQ54_17045"
     CDS             626..1747
                     /locus_tag="PLQ54_17045"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOS95025.1"
                     /translation="MAHSARDWWTRGLLVALIAVVGLASFRMGHSQGTASDDAERLGQ
                     AFAEAARTAGPAVVNISATRVVRGRAGLGLFDEFFPDLFRQPDMESTSLGSGVIIDPS
                     GYVVTNNHVVAGAQEVRVSLADGRELAAEVRGADPATDLAVLKIGAQGLPAARWADSD
                     RLQLGEWVVAIGNPYGLGQTITAGIVSAKHRVDVGVSAYADFIQTDAAINPGNSGGAL
                     VNVRGELVGINTAILSQSGGYQGIGFAIPSNLATEITGELIRTGKVDHGFLGVIVRPL
                     TEWLAQRIGQAGKAGELVVYRMYDGPAYRAGIRPGDIIESVDGTAVESERRLGKLISD
                     RKPGDSVRLIVRRKGKRYTFEVPLAAHPSDDSGRPIPGI"
BASE COUNT          253 a          597 c          700 g          268 t
ORIGIN      
        1 ccgggggctg ggtccccgtg cgggaggccg acgccgcggc cattgctgtc acggaggcgg
       61 gcgagtgggc gatccgcacg gctgccggcg aatcggtttc gtggcggcag accctccccg
      121 gcgtgggtgc gggcatcacg atcgaggccg ccgcgcgggt caccgcggtg ggggacgccg
      181 gcgaggggct gggccttcgg ctggcggtgc ctcgggccgg cggccaggtg gcccggtacg
      241 ggatcagcgt cacccgcacc ggcgtctact ggtacgcggg tgtgtggaca ccgctgatcg
      301 agtgggtcga tcatcacacg gccactcacc ggttccggct ggcgatccgc ccggacggcg
      361 gggcgcaggt ctatcgcgat ggcgagttgc tgggtgtccg gatgccggtc atcgagccgg
      421 gcgagagccc cgggccgttg gccgagtggg gcatcggcgc cgccgatgca tgcgaggtgg
      481 ccgtggccca ggtggccgtc gacacgtcgg gggcgtatgc tccatagggc cgcagcaaac
      541 cgcggccggc cgaggtcgtc tatcactggt ggacacgccg gggtgcccca ttcgcgggcg
      601 cgggcgtgcg cagaggagga ctccaatggc tcattcggcc agagactggt ggacccgggg
      661 gctcctggtg gccctcatcg ccgtggtcgg cctggcgagc ttccgcatgg gacacagcca
      721 gggcacggca tcggacgacg ccgagcgact cggccaggcc ttcgccgagg cggcgcgcac
      781 ggccgggccg gccgtggtga acatctccgc cacccgggtg gtccggggcc gcgcggggtt
      841 ggggctgttc gacgagttct tccccgacct cttccgccag ccggacatgg agagcacgag
      901 cctggggtcg ggggtcatca tcgacccgag cgggtacgtg gtcaccaaca accacgtggt
      961 ggccggtgcc caggaggtcc gggtctcgtt ggccgacggc cgcgagctgg ccgccgaggt
     1021 tcggggcgcc gaccccgcca ccgacctggc cgtgctcaag atcggcgccc aggggctccc
     1081 ggcggcgcgt tgggcggact cggatcgcct ccagctcggc gagtgggtcg tggccatcgg
     1141 taacccctac ggcctcggcc agaccatcac cgccggcatc gtgagcgcca agcaccgggt
     1201 cgatgtgggc gtgtcggcct atgcggactt catccagacc gatgcggcca tcaacccggg
     1261 caacagtggc ggggccctgg tgaatgttcg gggcgagctg gtggggatca acaccgcaat
     1321 cctcagccag agcggcggct accagggcat cggattcgcc atcccctcga acctggccac
     1381 agagatcacg ggagagctca ttcggaccgg gaaggtcgac catggcttcc tgggtgtgat
     1441 tgtccgtccg ctcaccgagt ggctcgccca gcgcatcggg caggccggca aggcgggcga
     1501 gttggtggtc taccggatgt acgacggtcc ggcctatcgg gcgggcatcc gccccggtga
     1561 catcatcgag agtgtcgacg gcaccgcggt ggagagcgag cggcggctcg gcaagctcat
     1621 ctcggaccgc aagccgggcg atagcgtgcg cctcatcgtc cgacgcaagg ggaagcggta
     1681 taccttcgag gtgcccctgg ccgcgcaccc ctccgacgac tcgggccggc ccatcccggg
     1741 gatctagcgg agtccgggat ctggggcatt ggcggtaact tagggctcca gtggcccatc
     1801 agcctggttg cggagggg
//
LOCUS       DAOSPL010001092         1816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_64791_length_1816_cov_3.825099, whole genome shotgun sequence.
ACCESSION   DAOSPL010001092 DAOSPL010000000
VERSION     DAOSPL010001092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64791_length_1816_cov_3.825099"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..>1816
                     /locus_tag="PLQ54_17050"
     CDS             2..>1816
                     /locus_tag="PLQ54_17050"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOS95026.1"
                     /translation="MDRGTETRQDTHELEQSARSMRAAALCAIHAGRSGHPGGSLSIM
                     DICAALYLNVARHCPQKPDWEDRDRIFFSAGHKAPALYSALAHAGYFPVQDIMSFRRY
                     GSMFQGHPHRLKTPGVEVSSGSLGQGLGIAVGAALAARLAGRPSRMYCIMGDGEQQEG
                     SVWEAAMAAGHFGLDNLCAIVDRNGLQIDGRTRDVMSLEPLEDKYRSFGWHVVTCDGH
                     DMGQILAAFDEAAKTKGKPTVIVANTVKGKGVSFMEDQAGWHGVPTKTREQLDQALAD
                     VGAEGFTADVVDAFIAASDAFAGRTAAVVDDLVPRFSRDYWWNTGSTMRVDMDPTRMG
                     FGRCLARIGDDPRIVTLHADISNSICITDFEKDHPERLNRVFSVGIAEQNMMQVAAGL
                     ALGGYIPITGTYGVFASGRCWDQIRTTICYGRLNVKIAGAHGGISVGPDGATHQSLEE
                     ISCMAVLPEMTLVVPCDSVETARASEAAILEVHGPAYIRYAREATPVVTTPDTPFELG
                     KANVIRFRGERDRFVDAFDTMLADSYKGEDESIAIVACGPMVPEAMRAAWILKAEHGI
                     ECRVLNVHTIRPLDEAAFVSAAADTGALVTCEEHQAGGL"
BASE COUNT          324 a          621 c          576 g          295 t
ORIGIN      
        1 tctggaccgt ggcaccgaga cgcgacaaga cactcacgaa ctggagcagt ccgcccgatc
       61 gatgcgggcg gcagccctgt gcgcgatcca cgcaggacgc tcggggcacc cgggcgggtc
      121 gctctcgatc atggatatct gtgccgcgct gtacctgaac gtagctcggc actgccccca
      181 gaaaccggac tgggaggacc gcgaccgcat cttcttctcc gccggccaca aggcacccgc
      241 gctctactcc gcactggccc acgccgggta ctttccagtt caggacatca tgagcttccg
      301 ccgctatggc tccatgttcc aggggcaccc tcaccggctg aagacgcccg gcgtcgaggt
      361 ctcgagcggc tctctcggcc aggggctcgg catcgccgtc ggtgctgcct tggcggcccg
      421 gctggccgga cgacccagtc gcatgtactg catcatgggc gatggcgagc aacaggaagg
      481 cagcgtgtgg gaagccgcca tggccgccgg gcatttcggc ctcgataacc tctgcgccat
      541 cgtcgaccga aatggcctgc agatcgacgg caggacccgg gatgtcatgt ccctggagcc
      601 tctcgaggac aagtaccgta gcttcggttg gcatgtcgtc acctgcgacg gccacgacat
      661 gggccagatc ctcgccgcct tcgacgaggc ggccaagacc aagggcaagc ctacagtcat
      721 cgtcgccaac acggtgaagg gcaaaggcgt gtccttcatg gaggaccagg ccggctggca
      781 cggtgtaccg acgaagaccc gcgagcaact cgatcaggcg ctggccgatg tcggcgccga
      841 aggcttcacg gccgacgtgg tggacgcatt catcgcggcg tccgacgctt tcgccgggcg
      901 caccgcagcc gtcgtcgacg acttggtacc gcgcttctcg cgtgactact ggtggaacac
      961 cggctccacc atgcgagtcg acatggaccc cacccgcatg gggttcggcc gctgcctggc
     1021 gcgcatcggc gacgatccgc gcatcgtcac gctccacgcc gacatatcga actcgatctg
     1081 tatcaccgat ttcgagaaag atcaccccga acgcctgaac cgcgtgttca gcgtcgggat
     1141 cgccgaacag aacatgatgc aggtggcggc cggactggcg ctgggcggct acatccccat
     1201 cactggtacc tatggcgtgt tcgccagtgg acggtgctgg gaccagatcc gcaccaccat
     1261 ctgctacggc cggctgaacg tgaagatcgc tggggcccac ggaggaatct ccgtcggccc
     1321 cgacggcgcc acacaccagt cgctcgagga gatctcatgc atggccgtgc tccccgagat
     1381 gactctggtc gtcccctgtg actcggtgga gaccgcgcgg gcctccgaag ccgccatcct
     1441 cgaagtccat ggacccgcct acatccgcta tgcccgggag gccacgccgg ttgtcacgac
     1501 acccgatacg cccttcgagc ttgggaaggc caatgtgatc cggtttcgcg gcgagagaga
     1561 ccgctttgtc gacgcattcg acaccatgct ggccgacagc tacaagggtg aagacgagtc
     1621 catcgccatc gtggcctgcg gccccatggt ccccgaggcc atgcgggccg cgtggatcct
     1681 gaaagccgag cacggtatcg agtgccgggt gctcaacgta cacaccattc ggccgctcga
     1741 cgaagccgct ttcgtcagcg cggccgccga cacgggtgcc ctggtgacct gcgaggagca
     1801 ccaggctggc gggctg
//
LOCUS       DAOSPL010001093         1816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_64808_length_1816_cov_2.852924, whole genome shotgun sequence.
ACCESSION   DAOSPL010001093 DAOSPL010000000
VERSION     DAOSPL010001093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64808_length_1816_cov_2.852924"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLQ54_17055"
     CDS             complement(<1..209)
                     /locus_tag="PLQ54_17055"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HOS95027.1"
                     /translation="MAADLTQETFVAAYRSAHTFNGRSQPEVWLLGVALNCHRSWMRK
                     TCRHEPPEELPPELPDPSPSAEQIA"
     gene            complement(526..1407)
                     /locus_tag="PLQ54_17060"
     CDS             complement(526..1407)
                     /locus_tag="PLQ54_17060"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOS95028.1"
                     /translation="MDDDLQRLVAKLESPSPDIRDAGARELIAVGEAALPHLYRCLES
                     PLVGTREAAARALGQIGNVEAVRALAPLLAAEPEAVVRAAASRALTQIGRSSPEAVHA
                     VAELLSADQWLLRKAAADALIQIGRGSVPALVRCLEDPDAPLRGYAAWVLGQIGEAAC
                     DAVAPLAALLDDAERSVVREAAWALSRIGPASVEALVDALERATPMAREYAARALGRI
                     QEPPPRAIEQLSRLLRDASCWVREASAIALGALGVLAQPAEPDLVRLLRDDEPRVRVA
                     AAQSLALIRPDSIPPDG"
     gene            complement(1582..1654)
                     /locus_tag="PLQ54_17065"
     tRNA            complement(1582..1654)
                     /locus_tag="PLQ54_17065"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1619..1621),aa:Cys,seq:gca)
BASE COUNT          299 a          652 c          616 g          249 t
ORIGIN      
        1 tcggcgatct gctcggccga tggcgatgga tccggcagct ccggcggaag ctcttccggt
       61 ggttcgtggc ggcaggtctt ccgcatccaa ctgcgatggc agttgagcgc cacccccagt
      121 agccacacct cgggctggga ccttccattg aacgtgtggg cactgcggta ggccgccacg
      181 aaggtctcct gggtcaggtc cgcagccacg tcccggtctc ccgttagccg ccacatcaga
      241 cggtagaccc ttcggtgctg ggaagctacc agggcctccc agacccaggg atcgcgtcgc
      301 ttgagtaagc gctgacgcga acgccccggc gcgtccgttc ccagactcag actcatcgcg
      361 ctcagccccc tgccggtcgt gcgaggcagc aacgacctgt tcaccccggg agtgccgggg
      421 ccaacgggcc gcccctcatg ggatacgtgg tcgctctgcg gtcaaacgtt ggcgccgcag
      481 tgcatggccc gccgccagca gccttgagac cgccgggacg acgggctagc cgtcgggagg
      541 gatcgagtcg gggcgaatga gcgccaggga ttgcgccgcc gcgacacgga cccgcggttc
      601 gtcgtcacgc agcagccgca caaggtccgg ctcggccggc tgggcgagca cacccaatgc
      661 gccgagggcg atggctgagg cctcccggac ccaacacgag gcatccctca acagccggga
      721 cagctgctcg atggcgcgtg gcgggggctc ctgaatgcgg cccagggcac gcgccgcgta
      781 ctcccgggcc atgggggtgg cccgctcgag cgcatccacc agcgcctcaa ccgaggccgg
      841 tccgatacga gagagagccc aggcggcctc gcggaccacc gatcgctccg catcgtcgag
      901 cagagcggcc aagggcgcaa cggcatcgca cgcggcttca ccgatctggc ccaggaccca
      961 ggccgcgtac ccacggaggg gggcgtccgg gtcctccagg caccgtacga gcgcaggcac
     1021 cgaaccccgg ccgatctgga tcagcgcatc ggccgccgcc ttgcgcagca gccattggtc
     1081 cgcgctcagc aactccgcga cggcatggac ggcctcgggc gatgagcggc cgatctgggt
     1141 cagcgcacga gaggccgccg cccggacgac ggcttcgggt tcggcggcca gcaagggagc
     1201 cagagcccga accgcctcca cattgccgat ctgccccaaa gccctggcgg ccgcctcgcg
     1261 ggtgcccacc aaggggctct cgaggcatcg gtacaggtgc ggcagtgccg cctctccaac
     1321 ggcgatcaac tcacgtgcgc ccgcgtcgcg tatgtccggc gagggactct cgagcttcgc
     1381 caccagccgc tgcaggtcgt cgtccatggt ccccccgtct tggcggcacc ccacttgccg
     1441 ctcgctcgat ctcagcgggg tgttcgcgcg ggcaccaggc atggcctgct ttgggagcca
     1501 cgacagtggg gcgacgcggg ctcggcacct cgcagtccgc ggtgccgccg cgccgagacg
     1561 aaggggggtt agcgagaagg gaggcggcgg tcggaatcga accgacgaat agaggttttg
     1621 cagacctccg ccttagccac ttggctacgc cgcctcgaac cggtgagaac ccgaccacgc
     1681 ggctcttggc ctcgagccgc ctgagacacg atgggtggcc gccgcacgac gaccacccac
     1741 gtcacctcaa tggagcggaa gacgggactc gaacccgcga ccccaacctt ggcaaggttg
     1801 tgctctacca actgag
//
LOCUS       DAOSPL010001094         1813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_64936_length_1813_cov_3.583049, whole genome shotgun sequence.
ACCESSION   DAOSPL010001094 DAOSPL010000000
VERSION     DAOSPL010001094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64936_length_1813_cov_3.583049"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLQ54_17070"
     CDS             complement(<1..385)
                     /locus_tag="PLQ54_17070"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HOS95029.1"
                     /translation="MPELPEIHHLAKQMDRALTGRRIEAVEIRQEKCLNLPAGRFREL
                     VEGKIIGRSKNRGKWVFTELHPEAWLLLNLGMGGDARLTRAGGDLPGTYQGRLDLDDG
                     SVFTLRFWWFGYIHAADDLTRHKMTC"
     gene            complement(417..1382)
                     /locus_tag="PLQ54_17075"
     CDS             complement(417..1382)
                     /locus_tag="PLQ54_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOS95030.1"
                     /translation="MSIHEIAAADLNVKQFIEEQVESIRQAVGDGWAINALSGGVDSS
                     AVTMLGHKALGNRLKTYFVDNALMREGEPQRVVALFAELGVPVELIDARDEFLAALRG
                     ITDPEEKREAVTQTFYKTVFGRLVRASGAKHLLQGTILTDVDETVAGIKRQHNVFEQL
                     GIDPQEAFGYHILEPLIQLRKDGVRKLAAGLGLPKSIYDRMPFPGPALAARIIGEVTA
                     ERLDIVRKATAITEEELEDTGAFQYMAILHEDRVTGMRDGQRDFGLQIEIRCWDSVDA
                     RRATPTRLPWETLERLADRILTGVPGVVSVTYGIAPKPPSTMEVV"
     gene            complement(1421..>1813)
                     /locus_tag="PLQ54_17080"
     CDS             complement(1421..>1813)
                     /locus_tag="PLQ54_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOS95031.1"
                     /translation="IIVAIDADANPSCPSGREVYIDGGRTPTGKDAVEFAREMAALGV
                     GQLLPTSKATDGAQGGYDLALTRGIADATGLPVIASGGAGTLEHFYEAVTEGHADAVL
                     AASVFHFGTFSIRQVKEYLRDHGVEVNL"
BASE COUNT          295 a          601 c          584 g          333 t
ORIGIN      
        1 cgcatgtcat cttgtgccgg gtcaggtcgt cagccgcgtg gatgtaccca aaccaccaga
       61 agcggagcgt gaagacggac ccatcgtcga ggtcgagtcg tccctgatag gtcccgggca
      121 ggtcgccgcc cgccctcgtg agccgggcgt cgccacccat cccgaggttc agcagcagcc
      181 acgcctcggg atgcagctcc gtgaagaccc acttaccccg gttcttggag cgcccgatga
      241 tcttgccctc gacgagttcc cggaaccggc ccgctggtag gttgaggcac ttctcttgcc
      301 gaatctcgac ggcctcgatg cgtcgccccg tcagcgcccg gtccatctgc ttcgccaggt
      361 ggtgtatctc gggcagttca ggcatgtgaa gagctccgtc cgctgatcgg gtttgctcaa
      421 acgacttcca tggtcgacgg tggcttgggc gcgatgccgt aggtgacgct gacgaccccc
      481 ggaacgccgg taaggatgcg gtcggcgaga cgctccagtg tctcccacgg taggcgtgtg
      541 ggcgtggcgc ggcgcgcgtc gacgctgtcc cagcagcgga tctcgatctg tagaccaaag
      601 tcccgctggc cgtcgcgcat accggtgaca cggtcctcgt gaagtatggc catgtactga
      661 aatgccccgg tgtcctcgag ttcctcctcg gtgatggccg tcgctttgcg cacgatgtcg
      721 agccgttcgg ccgtcacttc accgatgatc cgcgccgcca gcgccgggcc ggggaaaggc
      781 atgcggtcgt agatcgactt ggggagcccc aggcccgccg caagcttccg cacgccatcc
      841 ttgcggagct ggatcagggg ctcgaggatg tggtacccga atgcctcttg agggtcgatg
      901 cccagctgtt cgaacacgtt gtgctggcgc ttgatcccgg ccaccgtctc gtcaacatcc
      961 gtcaggatcg tgccctgcag caggtgcttg gcgccactgg cgcggaccag gcgcccgaac
     1021 acggtcttgt agaaggtctg cgtcacggcc tcgcgcttct cctccgggtc cgtgataccc
     1081 cgcagggcag ccaggaactc gtcacgggcg tcaatgagct ccacgggcac cccgagctcc
     1141 gcgaagagtg cgaccactcg ctggggctcg ccctcgcgca tcagggcgtt gtcgacgaag
     1201 taggtcttca ggcgattgcc gagggccttg tggccgagca tggtgaccgc cgatgagtcc
     1261 acgcctccgg agagagcgtt gatggcccac ccgtcaccca cggcctgacg gatgctctcg
     1321 acctgctcct cgatgaactg cttgacgttc agatcggcag cggcgatctc atgaatgctc
     1381 acgtgtgtgt tccctccaga tcaggagagg agccgccctc tcacagattc acctcaacgc
     1441 cgtggtcgcg caggtactcc ttaacttggc ggatagagaa ggtcccaaaa tggaacaccg
     1501 aggcggccaa gaccgcatca gcatggccct cagtgacggc ttcgtagaag tgctcaaggg
     1561 tacccgcccc gccactggca atgaccggca gtcccgtggc gtcggcgata ccccgcgtga
     1621 gcgcgaggtc gtacccgcct tgcgcaccgt cggtggcctt gctggtcggc agaagctgcc
     1681 cgacgcccag ggctgccatc tcgcgggcga actcgacggc gtccttgccg gtgggggtgc
     1741 gaccgccgtc gatatacact tcgcggcccg acgggcagct cgggttggca tcggcatcga
     1801 tggccacgat gat
//
LOCUS       DAOSPL010001095         1807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_65209_length_1807_cov_3.594178, whole genome shotgun sequence.
ACCESSION   DAOSPL010001095 DAOSPL010000000
VERSION     DAOSPL010001095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65209_length_1807_cov_3.594178"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..555)
                     /gene="flgC"
                     /locus_tag="PLQ54_17085"
     CDS             complement(112..555)
                     /gene="flgC"
                     /locus_tag="PLQ54_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOS95032.1"
                     /translation="MGIMAGVTASASGLTAQRLRMDVIADNIANASTTRTSSGGPFRR
                     RLVLFQARESGADSWLPVPMGGGEPKAGQGVRVVGIEEDLSPLRRVYDPGHPDADAEG
                     YVDLPNIHVVREMVDLISATRAYEANVSAISSAKEMATRALEIGA"
     gene            complement(567..956)
                     /gene="flgB"
                     /locus_tag="PLQ54_17090"
     CDS             complement(567..956)
                     /gene="flgB"
                     /locus_tag="PLQ54_17090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOS95033.1"
                     /translation="MGSGVTPVGWLFDDVPNRVLKLGMDVAALRHQVIANNIANVDTP
                     YFKASDVSFEEQLRAALSSGGAGAAELAEAGPVVYRVPATRFRIDGNTVDIDWETVKL
                     SQNTGRYNAQAAVLRRRLAMWRRVAGE"
     gene            complement(1283..>1807)
                     /locus_tag="PLQ54_17095"
     CDS             complement(1283..>1807)
                     /locus_tag="PLQ54_17095"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOS95034.1"
                     /translation="APGSGDARASAAPRSVTTAPPRRQTTLATTPEVPFVLFSVDSRV
                     FALPVSSVVEVVRADVECFSDESRPARTHPFVVGRMRFRDAQIPVVDLSGALGLEASG
                     SACDHVVVVDWRSARLGVAVSRVHEVTRVRPSAIEPPPPELAQMRGCVRGVFARGTET
                     VYVPHLEALVALGA"
BASE COUNT          314 a          654 c          574 g          265 t
ORIGIN      
        1 ggatctgccg ggccttgggc ggcggacggt gtggcgttcg ccacgggctg caggggcgtg
       61 atgttcatcc gggtccctca ctctcgcagg gccagagccc agaccacccg gctaggcccc
      121 aatctccagg gcccgcgttg ccatctcctt ggccgaggag atggcgctca cattggcctc
      181 ataggcgcga gtggccgaga tcaggtccac catctcgcgc acgacgtgga tgttgggcag
      241 gtcgacgtac ccctcggcgt ccgcatcggg gtgcccgggg tcgtacactc gccgcaaggg
      301 agacaggtcc tcctcgatcc ctaccacccg cactccctgc ccggccttcg gctcaccacc
      361 gcccatgggc acgggcagcc acgaatccgc ccccgactcc cgggcctgaa acagcaccaa
      421 tcggcgccgg aacgggccac cactgctggt tcgagtcgtg ctggcattgg cgatgttgtc
      481 ggcgatgaca tccatgcgca accgctgagc cgtgagcccc gacgcgctgg cggtcacgcc
      541 cgccatgatg cccatggtga tccctcctac tccccggcca cccgccgcca catggccaga
      601 cggcggcgca gcactgcggc ttgggcgttg taacgccccg tgttctggct cagcttcacg
      661 gtctcccagt cgatgtccac cgtgttgccg tcgatccgga accgcgtcgc aggcacacgg
      721 tacaccacgg gacccgcctc ggccagctcg gcagcgcccg cgccgccgct gctcagagcg
      781 gcgcgcaact gctcctcgaa cgacacatcc gaggccttga agtagggcgt gtcgacattg
      841 gcgatgttgt tggcgatgac ctgatgcctg agagccgcca catccatacc gagcttcagc
      901 acacggttcg gcacatcatc gaagagccaa cccaccggcg tcacccccga tcccatgcac
      961 acccgtcggc aacggagccc gacaggaact gcgcggccag tgcgccaggg gagggatgcg
     1021 ggcgatcgag gagcccagag ggcatcgctg gcagcagacg ccagcatcag cgcgaggaga
     1081 gcgtgtattc cggaggcacc gcatcagtca gtcagcggtg cgggtggcct ctgcctagca
     1141 gtccggcgca caacccacgc tctgtcctgg actccgtatc gctcccgact ccactcccct
     1201 tcgtgcgact ctcgcacgat tccccaccag attatggcct tcctctggcc cgcgctgcaa
     1261 aagttgggta aacgctgccg cgtcaggcac cgagggccac gagagcttcg aggtgaggca
     1321 cgtagacggt ctctgtaccg cgggcgaaca cgccccgtac gcagccgcgc atctgcgcca
     1381 gctcgggagg aggaggctcg atggccgaag ggcgcacccg agtcacctcg tgcaccctcg
     1441 acacggccac accgaggcga gccgaccgcc agtcgaccac gacaacgtgg tcgcatgccg
     1501 agccagaagc ctcgaggccc aacgctccgg agagatcgac gacggggatt tgcgcgtcgc
     1561 ggaacctcat ccggcccacg acaaaggggt gtgtgcgagc tggccgcgac tcgtcgctga
     1621 aacactcgac atccgcccgg accacctcca ccacactgct cacgggaagc gcgaacactc
     1681 ggctgtccac cgagaacagc acgaagggca cttccggcgt cgtcgccagg gtggtctggc
     1741 ggcgtggagg ggcagtcgtg accgagcgtg gcgccgcgct cgcgcgggcg tcgccagacc
     1801 caggagc
//
LOCUS       DAOSPL010001096         1806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_65268_length_1806_cov_3.718447, whole genome shotgun sequence.
ACCESSION   DAOSPL010001096 DAOSPL010000000
VERSION     DAOSPL010001096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65268_length_1806_cov_3.718447"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PLQ54_17100"
     CDS             <1..460
                     /locus_tag="PLQ54_17100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS95035.1"
                     /translation="VMLDNGFAYPGALVVGADSQPEYRAECDVLVRRLGLEARVVFYG
                     PVSWRRMPQIYSSARVTVIPSLSGEGTSLAALESMACGTPAVTTAVAGLLDLPAVHAD
                     PRPAPLAAALGQVLSDRDRVAADQARAVRERFSLDRWAEAWLRVVRAVAE"
     gene            457..1314
                     /locus_tag="PLQ54_17105"
     CDS             457..1314
                     /locus_tag="PLQ54_17105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS95036.1"
                     /translation="MISVCIVNWNTRDHLLACLASVAESRASAPLEVVVVDNASTDGS
                     VEAVRRDHPDVKLIANGENRGYAAGNNQAIAASTGEFVALLNPDVVLPPDGLEAMAEF
                     LRTHPEAAGVAPKLILPDGSVQRSVRDFPGPLEVFCELTRLSRVFPRSRRLARYWLPD
                     LDYDTPQPVPQPMASCLMLRRSDLERLGGFDEAFPIFFNDVDLSYRLHLAGRLTYLLS
                     SVCVRHFHGASTRQVRREMAWESARSLCAYYRKHYHGRLSPLIYYPLVSLIRLAGIVR
                     WLASVSPRR"
     gene            1402..1629
                     /locus_tag="PLQ54_17110"
     CDS             1402..1629
                     /locus_tag="PLQ54_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95037.1"
                     /translation="MRVPRSRLEIAQTHEGKKSQHGWLVINVAGLELEGVSRIHVNLD
                     EVEALRTKRDAVSSRLAELFGPANDDDLIPE"
BASE COUNT          257 a          630 c          598 g          321 t
ORIGIN      
        1 ggtcatgctg gacaacggct tcgcttatcc cggggccctc gtggtcgggg cggactccca
       61 gcccgagtat cgcgccgagt gcgatgtcct ggtccgccgg ctcggtctcg aagcccgcgt
      121 ggtcttctac ggcccggtga gctggcggcg catgccgcag atctactcgt ccgcccgcgt
      181 gactgtgatc ccgtcgctca gcggcgaggg cacctcactc gccgcgctcg agtccatggc
      241 ctgcgggacg cccgccgtga ccacggccgt cgcgggcctg ctcgacctgc cggctgtcca
      301 cgccgatccc cgccccgcgc ccctggcggc cgccctgggc caggtgcttt ccgaccgcga
      361 ccgcgtcgcc gccgaccagg ccagagctgt ccgcgagcgg ttcagcctcg atcggtgggc
      421 cgaggcctgg ctcagggtgg tccgggcggt ggcggagtga tctccgtctg catcgtgaac
      481 tggaacaccc gcgaccacct gctggcgtgc cttgccagcg tggccgagag ccgagccagc
      541 gcccccctgg aggtcgtggt tgtcgacaac gcgtcgaccg acggcagcgt cgaggccgtc
      601 cggcgtgacc atccggatgt gaagctgatc gccaacggcg agaacagggg ctatgcggca
      661 ggcaacaacc aagccattgc cgcctccacg ggcgagttcg tggccctgct caacccggat
      721 gtcgttctcc cacccgacgg cctggaggcc atggccgagt tcctccgcac tcacccggaa
      781 gccgctggag tggcgccgaa gctgatcctg cccgatggct ccgtgcagag gtccgtcagg
      841 gacttccccg gcccgctgga ggtgttctgc gagctgacga ggctctcccg cgttttcccg
      901 cggagccggc ggctggcccg ttactggttg ccggacctcg actacgacac accgcagccc
      961 gtgccccagc ccatggcgag ctgcctcatg ctgcgccgct cggacctgga gcgcctcggc
     1021 ggcttcgacg aagcattccc catcttcttc aacgatgtcg acctctcata ccggctccat
     1081 cttgctggaa gactcaccta cctcctctcc agcgtgtgcg tccgccactt ccacggagcc
     1141 tccacgcgcc aggtgcgtcg ggagatggcc tgggagtcgg cccgaagcct ctgcgcctac
     1201 taccggaagc actatcatgg gcgcctctcg cctctgatct actacccgct ggtgagcctc
     1261 atccgcctcg ctggtattgt gcgctggctg gcgtccgtat caccgcggcg ctgaccgtcc
     1321 attggcccaa gggttcggtg gctcgcgtgc cgaatgtact tcccgtcagt cccggaccca
     1381 cctgggatag ggggacatcc catgcgagtg ccccgaagcc gcctggagat cgcccagacc
     1441 cacgagggga agaagtcgca gcacggatgg ctcgtgatca acgtggcggg cctggagctt
     1501 gagggcgtgt cgaggatcca tgtgaacctc gatgaggtgg aggcgctgag gaccaagcgc
     1561 gatgccgtgt cgtcgcgcct ggccgagctg ttcggtccgg ccaacgatga tgacctgatt
     1621 ccggagtagt gcttggctcg atgaatgatg tcggaacgcg gaatctgctg gagatcgagg
     1681 ccctcaacca gcgcggtgga cggatgctca gcttcgtgga tctggtcgac gccgggacga
     1741 tgtcggccga tctggccgcc tttctgctgg cggcggttcg ctccggtgcg agcttcatca
     1801 ccgggg
//
LOCUS       DAOSPL010001097         1803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_65424_length_1803_cov_3.353547, whole genome shotgun sequence.
ACCESSION   DAOSPL010001097 DAOSPL010000000
VERSION     DAOSPL010001097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65424_length_1803_cov_3.353547"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            285..725
                     /locus_tag="PLQ54_17115"
     CDS             285..725
                     /locus_tag="PLQ54_17115"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOS95038.1"
                     /translation="MAVLIRRLPHPALWPSSVRHAESWAPPPPPPPPAPPGAPSGPPP
                     PPYGTPNKSKTTAGLLGLLSVFGVPGVHRIYLGYTAIGIVQLVAGPGLWFLIVLGSIF
                     TLGLCCFCIPLPVLASLWGLVEAILILAGQPPFDRDAQGRPLAP"
     gene            complement(804..>1803)
                     /locus_tag="PLQ54_17120"
     CDS             complement(804..>1803)
                     /locus_tag="PLQ54_17120"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOS95039.1"
                     /translation="VVVALMTVFSPDGGMVFLGQKGEGMATDEARPLSRPYAGPAGPA
                     GAASKSAQMKSMMGRRAAADAEAADATDHLGRRRADEMAKMAPSTGSPLAAPGAMAGA
                     APQSYREPSLQDQPMPWYDTSGGRQQELTRTLEVEVRKVEEAHDRAVSLVEKSGGFVL
                     SQDLRVEEGEPGVSRITARVPLDRIDGVVAQLRDMGKTLKLVGESEDRTREYYGRGGG
                     IRQSGASEDELVRQYEKATDPEQKRQLKAQIDALRQMTDAQKEQLLQMEERVRFALLD
                     LTLLEERGPMRFLGRVGENALKLLSWVLVSSVIWVPALVVVWVVRRRYGRHPAPPG"
BASE COUNT          253 a          676 c          566 g          308 t
ORIGIN      
        1 gtgccttcgt tggatggctg gttctgaccg agatccttcg cgccgcaggc gcggcactca
       61 ggatgacatc gctggcagca cgcccgagcg ccgtcggcct gccgcagcca atgagcatac
      121 ctgcgcaagt ccatgcagcc aggtccttgc cgtggctggg tgcgaaggag ctgaggcgct
      181 gccggccgaa ccacgagccc agagcgaggc gctccgcgcc cgggtctgtg gcagtggggc
      241 cctgtctgcc gcgcctcgga acctgccgac ggggagtgct gccgatggcc gttctgatac
      301 gcaggctgcc ccatccggcg ctctggccct caagcgttcg gcacgcggag tcctgggcgc
      361 cgccaccacc accgcctcct cccgcgcccc ccggagcgcc gtctgggcca ccaccccctc
      421 cctatgggac gccaaacaag tcgaagacca ccgctggtct cctgggattg ctcagcgtgt
      481 ttggagtccc aggggtccac cgcatctacc tgggctacac agccatcggc atcgtccagc
      541 tcgtggcagg gcccggcctg tggttcctca tcgtcctggg atcgatcttc accctggggt
      601 tgtgctgctt ctgcattccg ctcccggtct tggcgagtct gtggggtctc gtcgaggcca
      661 tcctgatcct cgccggtcag cccccctttg acagggatgc tcagggacgt ccgctggcgc
      721 cgtaggcctc aggttccgca cacgctctgg gggcgggcgc ccaaagcgcc cgcccccgct
      781 ctcgcgcctc gcgctccccg gggttatccc ggcggcgccg gatgccgacc atagcggcgc
      841 cgtaccaccc acacaacgac cagggccggg acccagatca ccgacgaaac cagtacccag
      901 ctcagcaact tcagcgcgtt ctcgcccacc cgaccgagga aacgcatcgg accgcgctcc
      961 tcgagcagag tcaagtccag cagcgcgaac ctcacgcgct cctccatctg cagcaactgc
     1021 tccttctgcg catcggtcat ctgacggagc gcgtcgatct gggccttaag ttggcgcttc
     1081 tgctcggggt ccgtcgcctt ctcatactgc ctgaccagtt cgtcctcgga cgccccggac
     1141 tgccgaatgc ctccgccgcg gccatagtac tcgcgggtcc tatcctcgct ctcacccacc
     1201 agcttcaggg tcttgcccat gtcgcgaagc tgggcgacaa ccccatcgat ccggtcgagc
     1261 ggaacccggg cggtgatgcg gctgacgccc ggctcgccct cttcgacccg caggtcctgg
     1321 gacaggacga accctcccga cttctcgacc agcgagacag ctcgatcatg ggcctcctcc
     1381 accttgcgga cttcgacctc gagcgtccgg gtgagctctt gctgccgccc gcccgaggtg
     1441 tcataccagg gcatgggttg gtcctgtagg ctgggctcgc gataggactg gggcgcggcg
     1501 ccggccatag cgccgggtgc cgcgagcggg gagcccgtgc ttggcgccat cttggccatc
     1561 tcgtcggccc tgcggcggcc gaggtgatcg gtggcgtcgg cggcttccgc gtccgcagcc
     1621 gcccgccgcc ccatcatcga cttcatctgg gcgctcttgc tcgcggcgcc agctgggccc
     1681 gcggggcccg catatgggcg cgacagcggc cgggcctcgt ccgtggccat cccctcaccc
     1741 ttttggccga gaaagaccat cccgccgtcg ggagagaaga cggtcatcag ggcgacgacc
     1801 acc
//
LOCUS       DAOSPL010001098         1803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_65430_length_1803_cov_2.787757, whole genome shotgun sequence.
ACCESSION   DAOSPL010001098 DAOSPL010000000
VERSION     DAOSPL010001098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65430_length_1803_cov_2.787757"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..>1803
                     /locus_tag="PLQ54_17125"
     CDS             23..>1803
                     /locus_tag="PLQ54_17125"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOS95040.1"
                     /translation="MHVVPAGVAHPDREGIVYDYFDVLARLWTTGYYQPQTDWCASRG
                     MTFTAHLCGEEQMMELIHGLNTDFFQSLSGVGEPGVDVIWRQVYFGCTTDFPRLGGSA
                     ANVWGKPRAMTESFAVFGSGTTYEQMKYVSDYQLVRSINDFFLMMWNYDCEGWRRLHH
                     PPDLSTDSTLYPYLRLYSEYIGRASTVMATGTPIADVAVYYPTRSAWLGDGASLDAAH
                     SMGRRLLERQRDFEWIDDKGLVDCCHPSAGGMRNLSGTLHRVFVIPPCDVISAAAADR
                     VREFVQAGGALVCYGRLPSRFGGYQPEPTTLNGLLRRMAAAPGEWVGEGQGRAVFVAD
                     ETAALAAIESAGPPRFGLREPISSLRLAGRDHGTFQVWFATNEGEEPIETELELAFVP
                     AVEEWDAETGACRPVAPIADGAGGCRVPLRLCPRASRVFVFGLGRPAQPVAAGPAHEL
                     LRLDGEWDWIFVHDGNAVHRGPLALWSELGRPEYSGDVDYAQSFELASVPSGRVVLDL
                     GRVRYMARVTLNGAALPARLWPPYAVDVTGLLRAGRNDLVVRVTNTPANRFFARPGDR
                     AAAEARGWFEGTYVGTYEAFEADSIPSG"
BASE COUNT          263 a          603 c          642 g          295 t
ORIGIN      
        1 tcgccccgat gagcgccgtg acgtgcacgt cgtgcccgca ggcgtggcac accccgaccg
       61 cgagggcatc gtctacgact acttcgatgt cctggccagg ctatggacca cgggctacta
      121 ccagccccag accgactggt gcgcctcgcg cgggatgacc ttcaccgccc acctctgtgg
      181 cgaagagcag atgatggaac tgatccacgg cctgaacacc gacttcttcc agagcctgag
      241 cggcgtcggt gagccgggtg tcgacgtcat ctggcggcag gtctacttcg gctgcacgac
      301 ggacttcccg cggctcgggg gatcggcagc gaacgtttgg ggcaagccca gggcaatgac
      361 ggagagcttc gccgtgttcg gctcgggcac cacctatgag cagatgaagt acgttagcga
      421 ctaccagctc gtccgcagca tcaacgactt cttcctgatg atgtggaact acgactgcga
      481 gggctggcgg cggctgcacc acccgcccga cctgagcacg gactcgacgc tctaccctta
      541 cctgcggctg tactccgagt acatcggccg ggcgagcacc gtcatggcca cgggcacacc
      601 catcgccgat gtggctgtct actaccccac gcggagcgcg tggctgggcg acggggcgtc
      661 actggatgcg gcgcacagca tggggcggcg gttgctggag cgtcagcgcg acttcgagtg
      721 gatcgacgat aagggcctcg ttgactgctg ccacccctcc gccggcggca tgaggaacct
      781 gagcggcact ctgcaccgtg tcttcgtcat tccgccgtgc gatgtgatca gcgccgcggc
      841 ggccgatcgg gtccgcgagt tcgtccaggc cggcggcgcg ctggtgtgct acggcaggct
      901 gccgtcacgc tttggagggt accagcccga gccgaccaca ctgaacggcc tgctccggcg
      961 catggcagcg gcgcccggcg aatgggtcgg cgagggccag ggccgggccg tcttcgtggc
     1021 ggatgaaacg gctgcactgg ccgcgatcga gtcggccggg ccgccgcggt tcgggctgcg
     1081 cgagcccatc agctcgcttc gcctggccgg gcgcgaccac gggaccttcc aggtctggtt
     1141 cgccaccaac gagggcgagg agcccatcga gacagagctc gagctcgcct tcgtcccggc
     1201 ggtcgaggag tgggatgccg agacgggcgc ctgccgcccc gtggctccga tcgccgacgg
     1261 cgcgggcggg tgccgggtcc ctctgcggct gtgccctcgg gcctcgcgtg tgttcgtgtt
     1321 cggtctcggc agaccggccc agccggtcgc tgcgggacct gcgcacgagc tactgcggct
     1381 cgacggcgaa tgggactgga tcttcgtcca cgacgggaac gcggtacatc gcgggccctt
     1441 ggcgctgtgg tcggaactgg ggcgcccgga gtactcgggc gatgtggact acgcgcagtc
     1501 attcgagctg gcgagtgtgc cctccgggcg cgtcgtgctc gatctcggcc gggtccgcta
     1561 catggcgcgc gtcacactga acggtgcggc gctgccggcg cggctctggc cgccctatgc
     1621 cgtcgatgtg accgggctgc tgcgagcggg ccgtaacgac ctggttgtcc gcgtgaccaa
     1681 cacaccggcc aaccggttct tcgcgcgacc cggggatcgt gccgcggccg aggccagagg
     1741 ctggttcgag ggcacgtacg tcggcacgta tgaggcattc gaggcggact cgatcccgag
     1801 tgg
//
LOCUS       DAOSPL010001099         1803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_65447_length_1803_cov_2.118993, whole genome shotgun sequence.
ACCESSION   DAOSPL010001099 DAOSPL010000000
VERSION     DAOSPL010001099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65447_length_1803_cov_2.118993"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1803
                     /locus_tag="PLQ54_17130"
     CDS             <1..>1803
                     /locus_tag="PLQ54_17130"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HOS95041.1"
                     /translation="MRVRSIPGPWPAAERLLVCVSPSPFSERLVRAARRLADELRAEW
                     FALYVEPTGRGGRAKADQYRVEKNLQLAEELGAKVATRHAPAVAAAVVGFARDHNVTK
                     IIVGKPLRPRWRELIRGSLVDQIARLSGPIDVYVISGPPGTTRAKPEDDPSRPRRWAP
                     YLASSLLVIATTLVGWPVHLAIEPTNLVILYLAAVVLAALRLGRGPAVLASVLGVAAL
                     DLFFVPPKLTFAVRDSQYLLTFLGLLVVGLVISTLAAQVRDQVDAGRRREEQTAALYS
                     LSRDLAAAVGLDAVAEAVIEHVGRSLGLSGRILLPDGGGLRVVSSDSGAELSDAELAV
                     ASWVYRNAKPAGEGTDTLASTAMRFLPLNTARGPRGVLALEGGSPDERADPGRRRLMD
                     AFASQAALAIERAQLAYEAGQVELLRATEELHAALLNSISHELRTPLASITGVLSTLR
                     QSPGGLDAVAPLGENARGELVEVAWEQTGRLNRLVGNLLDMTRLEAGAVRIRREPCDV
                     QDLVGAVLADMPEPWAGRVVTVSLAPDLPLVSADFVLLAQALSNVLDNAAKYSPPGSA
                     VEITAALASDGVEVCIADRGPGIPDVELAKKEGFS"
BASE COUNT          261 a          591 c          656 g          295 t
ORIGIN      
        1 catgcgggtt cggtcgatcc ctggcccctg gcccgcagcc gagcggctgc tggtgtgcgt
       61 cagcccgagc ccattcagtg aacggctggt tcgtgcggcc cgccggctcg ccgacgagct
      121 tcgggcggag tggttcgcgt tgtatgtcga gcccacgggg cgaggcgggc gcgcgaaagc
      181 cgaccagtat cgagtggaga agaacctgca gcttgcggag gagcttgggg ccaaggtggc
      241 cacgcgccac gcaccggccg tggcggcggc cgtcgtcggg ttcgcgcgcg accacaatgt
      301 gaccaagatc atcgtcggca agccgctgcg acctcgctgg cgcgagctga ttcgcggctc
      361 attggtcgac cagatcgccc gactgagcgg gccgatcgac gtctacgtga tcagcggccc
      421 cccggggacg actcgggcga agccggagga cgacccgtcg cgtcctcgcc gttgggcgcc
      481 ctacctggcg agcagcctgc tggtgatcgc gacgaccctg gtgggctggc cggttcacct
      541 ggcgatcgag cccacgaacc tcgtgatcct gtacctggcg gccgtggtac tggccgccct
      601 gcgactggga cgcgggccgg cagtgctggc ttccgtcctc ggggtggccg cactcgacct
      661 cttcttcgtg ccaccgaaac tcacgttcgc cgtgcgcgat agccagtacc ttctgacgtt
      721 cctcggcttg ctggttgtgg ggctcgtgat cagcacactg gccgcgcagg tccgcgatca
      781 ggttgatgcc ggccgccgga gggaggagca gaccgccgct ctgtactcac tcagccgcga
      841 cctcgcggcc gcagtgggac tggacgccgt ggcggaggcc gtgatcgagc atgtggggcg
      901 gagtctcgga ctcagtgggc ggatcctcct gccagacgga ggaggcttga gggtggtctc
      961 tagcgacagc ggtgcggagc tgagcgatgc cgagctcgcg gtcgcctcct gggtctaccg
     1021 gaacgcgaag ccggccggag agggcacgga cacgctggca tcgaccgcga tgcgctttct
     1081 gcccctgaac acggcccggg ggccccgggg tgttctcgca ctggaaggcg gtagccccga
     1141 tgagcgggcg gaccccggtc gtcgacggct gatggatgcc ttcgccagcc aggcagccct
     1201 ggccatcgag cgggcccagc ttgcgtatga ggctggacag gtcgagctgc tccgggcgac
     1261 cgaggagctc cacgccgccc tgttgaactc gatctcgcat gagctgcgca cgcccctggc
     1321 ctccatcacc ggggtgctga gcacacttcg gcagtcacct ggcggactcg acgcagtcgc
     1381 cccgctcggc gagaacgctc gcggcgagct cgtggaggtg gcgtgggagc agacgggccg
     1441 cctcaaccgt ctggtgggga acctgctcga catgactcgg ctggaggccg gtgcggtccg
     1501 aatccggcgc gaaccgtgcg acgtgcagga tctggtgggc gcagtcctcg ccgacatgcc
     1561 ggaaccgtgg gcgggaaggg tggtgaccgt gtctctcgca cccgacctgc cactcgtgtc
     1621 ggcggacttc gtgttgctcg cccaggcgct gtcgaacgtt ctcgacaacg cggcgaagta
     1681 ctcgccgccg ggatcggcgg tggagatcac agcagctcta gcctccgatg gcgttgaggt
     1741 ctgcatcgcc gaccgggggc ccggcatccc ggatgttgaa ctggccaaga aggaaggctt
     1801 ttc
//
LOCUS       DAOSPL010001100         1802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_65497_length_1802_cov_2.289067, whole genome shotgun sequence.
ACCESSION   DAOSPL010001100 DAOSPL010000000
VERSION     DAOSPL010001100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65497_length_1802_cov_2.289067"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1637)
                     /locus_tag="PLQ54_17135"
     CDS             complement(<1..1637)
                     /locus_tag="PLQ54_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95042.1"
                     /translation="MRAVLMPLTLTLLAAVCPAADITWTNVGPGGGGWIQAICLDPAD
                     PNRIHLGCDVGGYYRSDDMGGRWAIRNAGLTDYFVQCVAVCPDDPAVILLGCEGGVHR
                     SVDGGATWELCRDGFPAPDRWAFGAPIGSLVFDPSRPGVVYAGIGRPRWGKGGTGSVY
                     RSDDRGRTWRLCPRGEIPPEAIVMALDVSPNGSFILAATNQGLYRSGDGGQTWVDSGT
                     GLAHRRCVDVAISPSDPAICYLTLETLARGEAPFDGGVYRSSDGGVTWERCSDGLPDR
                     VGPEGGAYQLCSGYREMAVSPEDPDTVYIGSTAWVSPGVYKTTDGGASWSFAGPSADR
                     AGDDMGWIRFWGPTIECLAISPIEPNTLVCGTSGHVFVTRDGAATWRQAYCSETPDGR
                     VSGNGLEVTCLNNVAFDPLQRERLYACYFDIGLLISDDLGRSFRRQTQGMENEGNTFT
                     IRADPQMPGRLWASTGEWDTNVGDICRSVDGGLTWRVVGKPETGLPDGQVCTLLLDPD
                     SPLEARRLLATVKGHGVYVSDDGGDSWQAACEGLPEAV"
BASE COUNT          289 a          628 c          619 g          266 t
ORIGIN      
        1 acggcttcgg gcaggccctc gcacgcggct tgccaggagt ccccgccgtc gtcgctcacg
       61 tagacgccgt gtcccttcac ggtcgccagg agcctccgcg cctccagcgg gctatcagga
      121 tcgagcagca gcgtgcagac ctgaccatcc ggcaggccag tctcgggctt gccgacgacg
      181 cgccacgtca gaccgccgtc gacagagcgg cagatgtcgc ccacattcgt gtcccactcg
      241 ccggtgctgg cccagagcct gccgggcatc tggggatcgg cccgtatcgt gaacgtgttc
      301 ccctcgttct ccatgccctg ggtctgtctg cggaacgagc ggcccagatc gtcactgatc
      361 agtaggccga tgtcgaagta gcaggcatag agccgttcgc gttggagggg atcgaaggcg
      421 acgttgttga gacaggtcac ctcaaggccg ttgcccgaga cccggccatc gggcgtctcg
      481 gagcagtagg cctgccgcca cgtggcggcg ccgtcgcgcg tcacgaacac gtgtccggag
      541 gtcccgcaca ccagcgtgtt gggctcgatg ggcgagatgg cgaggcactc gatggtcggg
      601 ccccagaagc ggatccaccc catgtcgtct ccggcccggt cggccgacgg cccggcgaag
      661 ctccagctcg cgccgccatc ggtagtcttg tacacgcctg gcgagaccca ggccgtcgag
      721 ccgatgtaga cagtgtctgg gtcttcgggc gacacggcca tctcgcggta ccccgagcag
      781 agctgatagg cgcccccttc gggcccgacg cggtccggca agccgtcgga acacctctcc
      841 caggtcactc cgccatcgct gctgcggtat acgccgccat cgaagggtgc ctcgcctcgc
      901 gcgagagtct cgagggtcag atagcagatc gccgggtcag atgggctgat ggcgacgtcg
      961 acgcagcgcc ggtgggcaag gccggtcccg ctgtcgaccc atgtctgccc gccatcgccg
     1021 gagcgataga gcccctggtt cgtcgctgcg aggatgaacg agccgttggg ggacacgtcg
     1081 agcgccatga caatggcctc gggcgggatt tccccgcgcg ggcagaggcg ccaagtcctg
     1141 ccccgatcgt cactccggta gaccgaccca gtgccgccct tgccccagcg gggtcggccg
     1201 atacccgcgt agacgacgcc gggccgcgac gggtcgaaga ccagggaccc gatcggcgcg
     1261 ccaaacgccc atcgatcggg cgccgggaac ccgtctcggc agagctccca ggtggctccg
     1321 ccgtccactg atcggtggac gcccccctcg caaccgagga ggatcaccgc aggatcatcg
     1381 gggcacacag ccacgcactg aacgaagtag tcggtgaggc ccgcgttgcg gatcgcccac
     1441 cggccgccca tgtcgtcgct gcggtagtac ccgcccacgt cgcacccgag gtggatgcgg
     1501 ttcgggtctg cgggatcgag acagatggcc tggatccaac ctccaccgcc cgggccgaca
     1561 ttggtccacg tgatgtcggc ggccgggcag acggcagcaa gcagagtcag agtgaggggc
     1621 atgaggacag cacgcatcgc ggctcctctc tcggcgggag tgtgggtgca cgcttcgccg
     1681 cggccgccgg cggcaccggg ttctggctca tgatcgaggt cgaggcccag gccgcggcca
     1741 gcaccagggc caccgccggc agcgtgagca cggtcgagac gatggccgcg acgttcgagc
     1801 tc
//
LOCUS       DAOSPL010001101         1801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_65527_length_1801_cov_3.497136, whole genome shotgun sequence.
ACCESSION   DAOSPL010001101 DAOSPL010000000
VERSION     DAOSPL010001101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65527_length_1801_cov_3.497136"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1801)
                     /locus_tag="PLQ54_17140"
     CDS             complement(<1..>1801)
                     /locus_tag="PLQ54_17140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOS95043.1"
                     /translation="GGRVVWWNRAFEILLGLPESGLTRHGAGDFIRDSDLEFLRQAIA
                     EAESSERWPVRLRVRGRRYTGEPRAIDAVISPLSTPEGAETLLAVLRDATDDELLRDQ
                     ERLLANAVTAAAEIVIIIDTEGRITYTNPSAARLLGLAEADLIGRSLEQMVAEANSPE
                     AVEQMREEICLGRWRGDFALRTQTGATVWVRGSSGILCDDDGSALSVIIVGSDVTRER
                     QLQTQFMASEKLAAVGQLASGIAHELNNIIGAMSGYAQLAKASGTRQALDKLADTVID
                     SSARAAEIAENLLGFARPRKPRRERTRIDRPIDAALQLAASEFEKWDVQVSRSYAPSQ
                     PQLLIDVGMFQQVFLNLFINAVHAMPGGGRLSITVRHERGQPDERGQVVVLVSDTGCG
                     ISPEDLPRIFEPFFTRKQLEGDEGIRGTGLGLHVSRSIVEAHGGTITAESRLGEGTTM
                     VIRVPVVEGVAAHTADAIREVRPRRASAAAGLRVLVAEDDRTFRSLLADALALVECDV
                     VHVSDGARAIEVITAGGLDLVVSDLRLPRADGRMVLETAQHTVPPTPVIIVTGQSGTQ
                     VDQELLERGAYACLHKPIELPALLDLVEAVARTR"
BASE COUNT          299 a          629 c          574 g          299 t
ORIGIN      
        1 cgcgcgttct tgccacggcc tcaacgaggt ccagcaatgc cggcagctcg atgggcttgt
       61 gcaggcacgc gtaggccccc cgctcgagta gctcctgatc cacttgggtg cctgattggc
      121 ccgtgacaat gatgaccggc gtcggcggga ccgtgtgctg cgcggtctcc agcaccatgc
      181 gcccgtcggc tcgaggcaag cgcaggtcgc taaccaccag gtcgagcccg cccgccgtga
      241 tgacttcgat ggcccgggcc ccatcactga cgtggaccac atcgcactcc accagcgcca
      301 gagcgtcggc caggagcgac cggaacgtcc gatcgtcctc cgcaaccaga acacgcagcc
      361 ccgccgccgc cgacgcgcgc cgcgggcgca cctcgcggat tgcgtcggcg gtatgggcag
      421 ccactccctc gaccacggga acgcggatca ccatggtcgt gccttcgccc agacggcttt
      481 ctgccgtgat ggtgcccccg tgggcctcaa cgatggaccg agacacgtgc agtcccaggc
      541 cggtaccccg gatgccctca tcgccctcga gctgcttcct cgtaaagaac ggctcgaaga
      601 tgcgcggcag atcctcgggg ctgatgccac atccggtgtc ggacacgagt accaccacct
      661 gtccccgttc atccggctgg cctctctcgt ggcgcaccgt gatgctgagc cgcccgccac
      721 cgggcatggc atggacggcg ttaatgaaca ggttcagaaa gacctgctgg aacatgccga
      781 cgtcgatcag gagttggggc tggctgggcg cgtaggaccg gctgacctga acatcccact
      841 tctcgaactc actggccgcc agctgcagtg ccgcgtcgat gggccggtcg atgcgggtcc
      901 gttcgcgccg gggtttgcgc ggtcgggcga agcccagaag attctcggcg atctcggcgg
      961 cccgagccga tgagtcgatc accgtgtctg ccagcttatc gagcgcctgt ctggtgccac
     1021 tggctttggc gagctgagca tagccgctca tggcaccaat gatgttgttc agctcgtggg
     1081 caatgccaga cgccagctgc ccaaccgccg ccagtttctc cgaagccatg aactgcgtct
     1141 gtagctggcg ctcgcgcgtc acgtccgagc ccacgatgat caccgagagt gccgagccat
     1201 cgtcgtcgca caggatgccg gaggatccgc gcacccacac ggttgcgccg gtttgtgtcc
     1261 gcagggcgaa gtcgccacgc cagcgaccca ggcagatctc ctctcgcatc tgctcgaccg
     1321 cctccggcga gttggcctct gccaccatct gctccagcga ccgccctatc agatcggctt
     1381 ctgccaaacc gagcaggcgg gcagcggagg gattcgtgta ggtgatgcgc ccttcggtgt
     1441 cgatgatgat cacaatctcg gcggcggcgg tcacggcgtt ggcgagcagg cgctcctggt
     1501 cgcgcagcag ctcgtcatcg gttgcatcgc gcaggacggc cagtagcgtc tcggcaccct
     1561 ccggggtaga caggggcgag atcacggcgt cgatggctcg tggctcgccc gtgtaacgcc
     1621 gtccgcgaac ccgcaggcgg actggccaac gctcgctcga ctcggcctcg gcgatggcct
     1681 ggcggaggaa ctcgagatcg ctgtcacgga tgaagtcgcc tgcgccgtga cgcgtcagcc
     1741 cagactcagg caggcccagc agaatctcga aagcccgatt ccaccagacg acacgccccc
     1801 c
//
LOCUS       DAOSPL010001102         1801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_65548_length_1801_cov_2.426690, whole genome shotgun sequence.
ACCESSION   DAOSPL010001102 DAOSPL010000000
VERSION     DAOSPL010001102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65548_length_1801_cov_2.426690"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..>1801)
                     /locus_tag="PLQ54_17145"
     CDS             complement(54..>1801)
                     /locus_tag="PLQ54_17145"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOS95044.1"
                     /translation="TRRLDLFLSPLDPASLGAAARFVRELSRPRALERFDLNLRWVVY
                     QNTSGALAAPGGPAEVEEAARQAFVRRTRPELMADYLDERAKVGNSSYWDVPVRAVGL
                     DPETVRAGATQPEILEALMADARARQELNLPAGLTLLVENRELVDLHERGSSPEESVL
                     LTQQRTEAVLDRLGISSSSLTIAYAGHLNGQLEACGCPGNPFGGVAAMASALRDARQR
                     AGNFVFLSAGDMLPEMGDPARTTALLRALESMGLDALGLGDQELALGSDFVASLGQGS
                     PVPVVSANLSRAGEPLASRHIVLDRGLLRIGVFSVLAPEAFAFGTESYRRGIEIEDPI
                     SSARREAHVLRGEAGADVVVALSHLGAERDRELARMVPGIDVVIGAHTGRATHAEESE
                     GTTILVEAGRDGQFLGLLHLTLKDGAVESARNRLVLLDDSAGRDEAVQAIVADYKGAQ
                     EATFAGELAAAETIGTEFRPASCGACHAAEYEQWRSTGHGHAMATLASVGEERNPDCW
                     QCHSAPVAGAGGLRLQEVHCLACHDVGGITAQGHDPAAGKPVETRACLPCHTPLRSPN
                     FEWDRYRPFVAHKTP"
BASE COUNT          305 a          652 c          586 g          258 t
ORIGIN      
        1 agagaacccg gagccgggcg gtcccagacg ccggcggcgc ctgctggcag cccttagggc
       61 gtcttgtgcg ccacaaacgg ccggtaccgg tcccactcga agttggggct gcgcagcggc
      121 gtgtgacagg gcagacaggc tcgcgtttcg accggcttac ccgcagccgg atcatggccc
      181 tgggcggtga tgccgcccac atcatggcag gccaggcagt ggacttcctg gagcctcagg
      241 ccacccgcgc cggccacggg agccgagtgg cactgccagc agtcgggatt gcgctcctca
      301 cccaccgacg ccagggtcgc catggcgtgg ccgtggcccg ttgatctcca ctgctcatac
      361 tcggccgcgt ggcacgcgcc gcacgacgcc ggccggaact cggtcccgat ggtctccgcc
      421 gccgccagct cgccggcgaa cgtggcctcc tgggcgccct tgtagtccgc cacgatggcc
      481 tggacggcct cgtccctacc ggctgagtcg tccagcaaga cgaggcgatt gcgggccgac
      541 tcgaccgcac cgtccttcag cgtcaggtgg agcaagccca ggaactgccc atcccggccc
      601 gcctcgacca gtatggttgt cccttcggac tcctcagcat gagtcgcccg gcccgtgtgc
      661 gcgccgatca ccacatcgat gcccggcacc atccgggcaa gctcccggtc acgctcggca
      721 ccgaggtgcg agagggcgac gaccacatcg gcccccgcct cgccgcgaag aacgtgggcc
      781 tcacgccgcg ccgaactgat tggatcctcg atctcaatcc cacgccggta gctttcggtg
      841 ccgaaggcga aggcctcagg cgccagcacc gagaacacgc cgatacgcaa cagaccacgg
      901 tccagcacga tgtgccggct ggccagcggc tcacccgccc tggagagatt agccgagacg
      961 acaggcaccg gcgatccctg cccgagcgac gccacgaaat cggaacccag agccagttcc
     1021 tgatcgccga gtcctagcgc atcgagtccc atcgactcga gggctcgcag cagggctgtg
     1081 gtgcgagccg ggtcacccat ctcgggcagc atgtcgccag cactgagaaa gacgaagttg
     1141 cccgcgcgct gccgtgcatc gcgaagcgct gaggccatgg cggccacgcc gccgaacggg
     1201 ttgccggggc atccgcaggc ctcgagctga ccgttgaggt ggcccgcata cgcaatggtc
     1261 agcgagctcg agctgatgcc caaccggtcc agcacggcct cggtcctctg ctgcgtgagc
     1321 agaacagact cctcggggct ggaaccccgc tcatgaaggt ccacgagctc gcgattctcg
     1381 acgagcagag tcagaccggc cggaaggtta agctcctggc gcgctcgggc gtccgccatc
     1441 agcgcctcga ggatctcggg ttgggtcgcc ccggcgcgaa ccgtttcggg gtcgagaccg
     1501 acagcacgca caggcacatc ccagtacgag ctgttaccga ccttggcccg ctcgtcgagg
     1561 tagtcggcca tcagttcggg ccgcgtgcgg cgtacgaatg cctgccgcgc cgcctcctcc
     1621 acctcggccg gtccgccggg tgccgccagg gccccgctgg tgttctgata caccacccac
     1681 cgaaggttca ggtcgaagcg ctccaacgct cgaggcctcg atagctcccg aacgaaacga
     1741 gcggcggcgc ccagcgatgc tggatcaaga gggctgagga acaggtcgag ccgccgcgtc
     1801 t
//
LOCUS       DAOSPL010001103         1800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_65602_length_1800_cov_2.505444, whole genome shotgun sequence.
ACCESSION   DAOSPL010001103 DAOSPL010000000
VERSION     DAOSPL010001103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65602_length_1800_cov_2.505444"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PLQ54_17150"
     CDS             complement(<1..297)
                     /locus_tag="PLQ54_17150"
                     /inference="COORDINATES: protein motif:HMM:NF014456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HOS95045.1"
                     /translation="MRVLRADFAGFCPGVERALALVEAALAQGHPLCSLGPLIHNDQV
                     VRRLADEGLRCADVLDDVRPGETLVVRTHGVPASVTRQAAARGVRVIDATCRFVR"
     gene            complement(381..>1800)
                     /locus_tag="PLQ54_17155"
     CDS             complement(381..>1800)
                     /locus_tag="PLQ54_17155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS95046.1"
                     /translation="GPQGATGATGATGPEGIKTEALKLPIEELTGLVFGGLIAGFRHQ
                     AANITYWRMQRYWEDGHWPRVLGMLKLTCFLDPHFQAAWSMLGWHQAYNLSAEMDKQT
                     FPPIPPERYVSDGMKSYERGLDYNPGSYMLYEDAAWTAFDKAGDMRIATEFLEKALLT
                     WDPEPDVTKKGPLVYTRMLGHAGEKALDMEAALEAYQRVLRMDPDDSVGIGATFTIRE
                     RYLPAIRAAERGDIDRAIRLLTEVQWRRPGDPFVPGLLARIYEKAKKDKGAALAAWER
                     SGREWRQKVARAEAYRLMAELGRLDDLGPLVDVRKLYEYVVWQMNPGGMGVLINIRLG
                     PDGKTISDPRVEDGQVVRIDPSPDPMGRVASLPPGTARWFLNGVLIAEDNTSPYTCTL
                     PLEPYRGSEMDKLLLKVDFVPDGGGRVLWDVVDLVPSWVKPKTEGRPGGPGMPQKMQM
                     PGMPGPGGPAAPPSPSGGGQSH"
BASE COUNT          304 a          649 c          552 g          295 t
ORIGIN      
        1 ccgcacgaac cggcatgtcg cgtcgatcac gcgaactcca cgcgcagcgg cctgacgggt
       61 caccgatgcc ggaactccgt gggtgcgcac cacgagagtc tcgccaggtc gcacatcatc
      121 gaggacgtcc gcgcaccgaa gcccctcatc ggccagccgc cggaccactt ggtcgttgtg
      181 gatcagcggg cccagactgc acagcggatg gccctgagcc aacgccgcct cgaccagcgc
      241 caatgcccgt tccactcccg ggcagaaccc ggcgaaatcc gcgcgaagca cccgcaccgc
      301 acgcgaactc ggcccttcgg ccgacccggt cctggcgctg ccctcgctca gcatgtcggt
      361 tcccgctacg gaatcggggc tcagtgggac tgcccgccgc ccgacggaga tggcggggcc
      421 gccggtccgc ccggcccggg catgccgggc atctgcatct tctggggcat tcccggtccc
      481 ccggggcgcc cctcggtctt gggcttcacc cacgacggca ccagatccac cacgtcccac
      541 agaacacgcc cgcctccatc gggcacaaag tccaccttca gcagcaactt gtccatctcg
      601 ctgccacgat agggttcgag cggcagtgtg cacgtgtagg gtgatgtgtt gtcctcggcg
      661 atcagcaccc cgttgaggaa ccagcgcgcc gtacccggag gaagcgaggc cacacggccc
      721 atggggtcgg gcgaggggtc gatacgaacc acctgcccgt cctccactcg gggatcggag
      781 atggtcttgc cgtcgggccc caggcggatg ttgatgagca cacccatgcc gcccgggttc
      841 atctgccaca cgacgtactc atagagcttt cggacatcga ccaggggacc cagatcgtcc
      901 agccgcccga gctcagccat cagacggtac gcctccgccc gggcgacctt ctgccgccac
      961 tcacggccgc tgcgctccca ggccgccagc gctgccccct tgtccttctt ggccttctca
     1021 tagatcctcg cgagcagccc cggcacgaac ggatcgccag gccgccgcca ttgcacttcg
     1081 gtcagcaaac ggatggctcg gtcgatgtct ccacgctcag ccgcccgtat ggccggcaga
     1141 tagcgctcgc ggatggtgaa cgtggccccg atccccacac tgtcgtcggg gtccatgcgc
     1201 agcacgcgct gataggcctc aagcgcggcc tccatgtcca gcgccttctc gcccgcgtgg
     1261 cccagcatcc gggtgtacac cagggggccc ttcttcgtaa catcaggctc cgggtcccac
     1321 gtcagcaacg ccttctcgag gaactcggtg gcaatccgca tgtcgccggc cttgtcaaat
     1381 gcggtccagg cggcatcctc gtacagcatg taggaacccg ggttgtagtc gaggccgcgc
     1441 tcataggact tcatgccgtc ggagacgtac cgctcgggag gtatgggagg aaacgtctgc
     1501 ttgtccatct cggccgacag gttgtaggcc tggtgccaac cgagcatcga ccacgccgcc
     1561 tggaagtgtg gatcgaggaa gcaggtcagt ttgagcatgc cgagaacgcg cggccagtga
     1621 ccatcctccc aataacgctg catgcgccaa taggtgatgt tggcggcctg atggcggaac
     1681 ccggcaataa ggccaccgaa gaccagaccc gtcagctcct cgatcgggag cttgagggct
     1741 tccgtcttga tcccctcggg accggtcgcg ccggttgcgc cggttgcgcc ctgtggtcct
//
LOCUS       DAOSPL010001104         1799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_65644_length_1799_cov_2.658257, whole genome shotgun sequence.
ACCESSION   DAOSPL010001104 DAOSPL010000000
VERSION     DAOSPL010001104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65644_length_1799_cov_2.658257"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="PLQ54_17160"
     CDS             complement(<1..928)
                     /locus_tag="PLQ54_17160"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOS95047.1"
                     /translation="MLVTITAALALAAALPSFPPANPKAVREVEAGSRTVANAAWWGF
                     DPVNSTAALQGAINSGATKVIVPNVGAEWIVDPLFLVSDQEIVFERGVVIAARQGGFP
                     GLGDSLLTADKQRNLTIRGYGATLRMQKPEYTQGEWRMCINLGSCTNVRIYGLTLRDS
                     GGDGIYLGNTDSNQPYCREIEVRDCVLDNHRRQGMSVISAQDLMVENCVFRNTDGTAP
                     QAGVDLEPNLPAERLVNCVFRNCVMEDNTGPGILVYLFNLTSESKPVSVRFEDCAITS
                     RQGSGIQLAGTRPGSQRGQVVFEDCAVEGCQDI"
     gene            complement(1148..>1799)
                     /locus_tag="PLQ54_17165"
     CDS             complement(1148..>1799)
                     /locus_tag="PLQ54_17165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95048.1"
                     /translation="KSANVDFLRDIDHRGRTIIVWSLTPETQSTRMEALSATTEERIE
                     AARKCQSWGYPIRFKYKPIVPVNGWRDEIAAMTRAVFERTRPDVISLFTLAWMSYEEL
                     VRLADLSLLDPRFVAGAAEAAESMGTSKVRPFPHDLRREVYEFCLREIRKYDAEVPVS
                     LSTETLDMWKDMSPLLGLTPGTYPCGCGPTSTPGLSRLPCSPWSVADPVPVSVEGR"
BASE COUNT          291 a          631 c          570 g          307 t
ORIGIN      
        1 ctatgtcctg gcagccctca accgcgcagt cctcaaagac gacctggccg cgctggctgc
       61 cgggacgcgt tcccgcgagc tgaatgccgg atccctgccg gctggtgatg gcgcagtcct
      121 cgaagcggac ggacacgggc ttggattcac tcgtgaggtt gaacaggtag accagtatcc
      181 cggggcccgt gttgtcctcc atcacgcagt tgcgaaagac acaattcact agccgctcag
      241 ccggcagatt gggctccagg tcgacgccag cctgcggtgc cgttccatcg gtgttgcgga
      301 agacgcagtt ctcaaccatc aggtcctgcg ccgatatgac gctcatgccc tgccgccggt
      361 ggttgtccag cacgcagtcc cgaacctcga tctcgcggca gtagggttgg ttcgagtccg
      421 tgttgcccag atagatcccg tcgccgccgc tgtccctcag cgtcagcccg tagatgcgga
      481 cgttggtgca gctaccgagg ttgatgcaca tgcgccactc gccctgggtg tactcgggct
      541 tctgcatcct cagcgtcgcg ccgtagccgc ggatggtgag gttccgctgc ttgtcggccg
      601 tcagcagcga gtctcccagc ccggggaaac ctccctggcg tgcggcgatg accacgccac
      661 gctcgaagac gatctcctga tcgctcacga ggaagagagg gtcgacgatc cactctgccc
      721 cgacgttggg cacgatcacc ttggtcgcgc ccgagttgat ggctccctgc agcgccgcgg
      781 tggagttcac cgggtcgaac ccccaccagg ccgcgttcgc gacggtccgc gagccggcct
      841 cgacctcacg gacggccttc gggttggcgg gcggaaacga gggaagagcg gcagcaagcg
      901 cgagcgccgc ggtgatggtc acaagcatgg tcgattcccc ctgcgaacgt gctgcctgcc
      961 cactgccgcc cgagcccacg gcccctactc gacgcctggg ttcccgcgtg ccccatgtgg
     1021 ccatcgcggc ccgtgcctgg ccgcatcgag cgcacccacg cggcagcgga gtcagctcca
     1081 ggggaccagt cccctggcga ggcgcgggca gcgcctcgcg gtcgtccgac gtgggcgttc
     1141 gcgctaccta acgcccctcc acgctcaccg gcaccggatc ggccaccgac cacgggctgc
     1201 acgggagcct cgagagcccc ggggtcgacg tcggaccaca cccgcaaggg taggtaccgg
     1261 gtgtgaggcc aagcaagggc gacatgtcct tccacatgtc caacgtctcc gtcgacaacg
     1321 agacgggaac ctcggcatcg tacttgcgga tctcccgcag gcagaactca tacacctcgc
     1381 gacgcaggtc gtgtgggaat ggccgcacct tgctggtgcc catcgactcg gcggcctcgg
     1441 ccgcgcccgc aacgaaccgc gggtcgagga gtgataggtc cgccagccgg accagctcct
     1501 cgtagctcat ccatgccaga gtgaacagcg agatcacatc gggcctggtc cgctcgaaca
     1561 ccgcccgcgt catcgccgcg atctcatcgc gccacccgtt caccggcacg atgggcttgt
     1621 acttgaaccg gatgggatag ccccacgact ggcatttgcg ggccgcctcg attcgctcct
     1681 ccgtcgtcgc cgacagggcc tccatgcgcg tgctctgggt ctcgggtgtc aggctccaca
     1741 cgatgattgt gcgtccccga tggtcgatgt ctcgcaggaa gtcgacattg gcgctcttc
//
LOCUS       DAOSPL010001105         1797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_65714_length_1797_cov_3.863375, whole genome shotgun sequence.
ACCESSION   DAOSPL010001105 DAOSPL010000000
VERSION     DAOSPL010001105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65714_length_1797_cov_3.863375"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PLQ54_17170"
     CDS             <1..423
                     /locus_tag="PLQ54_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95049.1"
                     /translation="QCELRGHHGGHAGQPHVDVMAAGVAKWKSEGAVGLNTFANPACG
                     EPDRAGAVRAFASLRDGAEKSLRVPNPLRDYLRDRMLSICAEQDLTMAVHAGMWGDFR
                     DLDSAHIIRPIQRHPRTRFGLYHLEMPEPRQTMVIARN"
     gene            complement(625..1050)
                     /locus_tag="PLQ54_17175"
     CDS             complement(625..1050)
                     /locus_tag="PLQ54_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95050.1"
                     /translation="MTTHRPMLLVLLAFAAVALAGCGTTDNTDDGYAVDPDSDLVATW
                     RFASAFIDQVPVSLPNRFLIINADGTWELSDNQDTLQRGSWGVKDNRVRFTADQASPD
                     AIWSGTFTPRWTVTGDVLQLVGNDGAGSYHVHAYQRQAL"
     gene            complement(1081..1482)
                     /locus_tag="PLQ54_17180"
     CDS             complement(1081..1482)
                     /locus_tag="PLQ54_17180"
                     /inference="COORDINATES: protein motif:HMM:NF013399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HOS95051.1"
                     /translation="MGDSLGGTPGGHLSSPASIRGFVSRIDPARHYAGGQSVRLTLHF
                     RVHPGLLNGLMAGLPARGGQELQVNSLDPARLRAARRDPAAHRDIVVRVAGFSARFVE
                     LSPLEQAELIERAERAVSRTPFAPEASYPVD"
BASE COUNT          324 a          587 c          609 g          277 t
ORIGIN      
        1 cagtgcgaac tacgaggcca tcacggcggc catgcaggcc aaccacacgt cgatgtcatg
       61 gccgccggcg tggccaagtg gaagtccgag ggtgcagttg gcctgaatac attcgccaac
      121 cccgcgtgcg gcgagcccga ccgcgcgggt gcggtccgcg ccttcgccag tctgcgcgat
      181 ggtgcggaga agagcctgcg cgtcccgaac cctctccgcg actacctgcg cgaccggatg
      241 ctatccatct gcgccgaaca agacttgacc atggcggtcc acgccggcat gtggggcgac
      301 ttccgcgacc tggattccgc ccacatcatc cgacccattc agcggcaccc acgcacgcgc
      361 ttcggtctct accacctcga gatgcctgag ccgcgccaga ccatggtcat cgcgaggaac
      421 tgagcgaacg tgtggctgaa cctcgcttgg tgacacgtca tctcgcaggc catgacgcgg
      481 tcggcggtgc acgagcgtat cgacgccggt ctgatgaccg gggcccaggc cctcaacatc
      541 gccaccaagt ggctctggca caacccgaag gagtgctacc ggctcaacat ggagaaaggc
      601 cggaccaccc aacccacggc cagcctacag cgcttgccgt tggtaagcgt ggacgtggta
      661 ggaaccagcc ccgtcattgc cgacgagctg gagcacgtcg ccggtcacgg tccagcgagg
      721 ggtgaatgtg ccgctccaga tcgcatccgg ggaggcctga tcggcggtga accggacgcg
      781 gttgtccttc acgccccacg agccgcgctg aagggtgtcc tgattgtccg aaagctccca
      841 cgttccatcg gcgttgatga tcaggaaccg gttgggaagc gacacgggaa cctggtcgat
      901 gaacgcgctg gcgaaccgcc aggtcgcaac gagatcgctg tcggggtcga ccgcgtagcc
      961 gtcgtcagtg ttgtccgtgg tgccgcagcc tgcgagggcg acggctgcga atgcgagcag
     1021 cacaaggagc atgggtctgt gtgtagtcat gggtctcctg acgtgaggga gatcaggtgc
     1081 tcagtctaca gggtaagacg cctcaggcgc gaagggggtt cgtgacaccg cgcgttcggc
     1141 ccgttcgatc agctcggcct gctcgagtgg cgacagctca acgaaacggg cggagaatcc
     1201 ggccacgcgg accacgatgt cgcggtgcgc agccggatcg cggcgcgcgg cacgcaaccg
     1261 ggcggggtcc aggctgttga cctgcagctc ctgcccgccc cgggcaggaa ggccagccat
     1321 gaggccattc agcaggcctg gatggacacg gaagtgcagt gtgagacgca cgctttggcc
     1381 cccggcgtag tggcgtgccg ggtcgatgcg cgagacgaac ccccggatcg aagccggact
     1441 gctgaggtgg ccgcccggcg tgccgccgag gctgtcgccc acgggtctcc ccgatcgagg
     1501 acccatcgac atagtgcagc gagcgtacga caaggcacgg tgtatgccgg agcagaccgc
     1561 gcccggcccc aacctcgtcg aggatccgtg tccggctctc gagcagccag agcagccaac
     1621 ggcagagcga ctcatgggcc gagctgcact tgggtgcgtc tagcattcgc tgcctcagac
     1681 cctcgtctgg atggtcttgc cgcatggagt gcacgatagc gtccaggtcg accggattcg
     1741 cgccgaagcc gaggcgctcg atggcggtga gggagtcggc ggcttccacc agtcccc
//
LOCUS       DAOSPL010001106         1796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_65791_length_1796_cov_2.389431, whole genome shotgun sequence.
ACCESSION   DAOSPL010001106 DAOSPL010000000
VERSION     DAOSPL010001106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65791_length_1796_cov_2.389431"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="PLQ54_17185"
     CDS             <1..813
                     /locus_tag="PLQ54_17185"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOS95052.1"
                     /translation="EFVADGALQLRVRCVYRIGEASTLTQDMVFHTHTPRIDFETLID
                     WKEKRKLLKAAFDLQVLAENARHEIQYGHVERPTHQNLPGDRARFEVCAHKWTDLSEN
                     GFGVALLNDCKYGVSVRRGTVGLSLLKSSVHPDPRGDEGRHHMTYSLLPHACAFGVKA
                     VVRPAYELNVPLITHMASDAAEPVASLVAVDSPHVIIESVKWAESGEGFVVRLYDAAK
                     LGHKVRLQFGLPVKSVAETNMLEENPKRVSVRRGGVELYMRPFEIKTLVCAV"
     gene            complement(940..1770)
                     /locus_tag="PLQ54_17190"
     CDS             complement(940..1770)
                     /locus_tag="PLQ54_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOS95053.1"
                     /translation="MGYVDAHAHVWTGDTAAYPLAPGYGVSDMVPPSFTPEELFGHCR
                     PCGVDRVVLIQMSFYGLDNRYMLDAIATYPDALRGVAVVDPHRPDLAEEAQRLRDAGV
                     RGFRVYPGPAPASDWLRDGSYDALFCTAGRLGMAVCPLIDPEYLSAVGWVAERHPETT
                     IVVDHLARVGMRTMAEQHIEDLCALADHPNCLVKVSAFYALGARKSPHDDLAPLIRRV
                     YDAFGPGRLMWATDCPYQVMDETYEDSIGLIRERLDFLGPSDRQSLLCDTAERVFFGA
                     "
BASE COUNT          302 a          595 c          613 g          286 t
ORIGIN      
        1 gagttcgtcg cggacggcgc cctacagctt cgcgtgcgtt gcgtctatcg catcggcgag
       61 gcgtcgacgc tgacgcagga catggtgttc cacacgcaca cgccgcggat cgacttcgag
      121 accctcatcg actggaagga gaagcggaag ctgctcaagg ctgccttcga cctccaggtg
      181 cttgccgaga acgcgcgcca cgagatccag tacgggcatg tcgagcgccc cactcatcag
      241 aacctgccgg gtgaccgcgc gcgtttcgag gtctgcgccc acaagtggac cgacctctcg
      301 gagaacggct tcggcgtggc cctgctcaac gactgcaagt acggggtgag cgtccggcgt
      361 gggacggtgg ggctgagcct cctcaaatcg agcgtgcacc ccgatccgag gggcgacgag
      421 ggccgtcacc acatgaccta ctccctgctg ccccacgcct gtgcgttcgg cgtgaaggcg
      481 gtcgtccggc cggcgtacga gctgaacgtg cctctgatca cccacatggc gtccgatgcc
      541 gccgagccgg ttgcgagctt ggtcgccgtc gactcgcccc atgtgatcat cgagtcagtc
      601 aagtgggccg agagcggcga gggattcgtc gtccgcctct acgatgcggc caaactgggc
      661 cataaggtgc ggctccagtt cggcctgccg gtgaagtcgg tggccgagac caacatgctc
      721 gaggagaacc cgaagcgcgt cagcgtccgc agaggtggcg tcgagctcta tatgcggccg
      781 ttcgagatca agacgctggt gtgcgcggtg taggcggcat tcagacggga acgtcggcgg
      841 gggccgggca tggtccccgc cgtctcctgt agtcgaggtc tggccccggg gccatcatgg
      901 tgtgcacagt ggcgtcgcgc gaggcccgag aacggttcgc tatgccccga agaacacccg
      961 ctcggccgtg tcgcacagca gcgactgccg gtccgacggg cccaggaagt ccaggcgctc
     1021 gcggatgagc ccgatactgt cctcataggt ctcgtccatc acctggtacg ggcagtcggt
     1081 ggcccacatg agccgaccgg gcccgaatgc gtcgtagacg cgccggatca gcggcgccag
     1141 gtcgtcatgc ggcgacttcc tggcccccag cgcatagaat gccgagacct tcaccaggca
     1201 gttggggtgg tcagccaggg cgcacaggtc ctcgatatgc tgctccgcca tcgtccgcat
     1261 cccgactcgc gccaggtggt ccacgacgat ggtcgtctcg gggtgccgct ccgccaccca
     1321 gccgacggct gacaggtact cggggtcgat cagtgggcag accgccatcc cgagccggcc
     1381 ggcagtgcaa aagagcgcat cgtagctgcc atctcggagc caatcggagg ccggggcggg
     1441 cccagggtag actcggaatc ccctgacccc ggcgtcgcgc aggcgttggg cctcctcagc
     1501 cagatcgggc cgatgggggt cgaccacggc cacgccgcgc aaggcgtccg ggtacgttgc
     1561 gatggcgtcg agcatgtacc ggttatcgag cccgtagaag ctcatctgga tgagcacgac
     1621 tcggtcgacg ccgcacggcc ggcagtgacc gaacagctcc tcgggcgtga atgagggtgg
     1681 caccatgtca gagaccccgt agcccggcgc cagagggtac gcggccgtgt cgcctgtcca
     1741 cacgtgagcg tgtgcgtcga catatcccac gggctagccc tccgcccggc ggatct
//
LOCUS       DAOSPL010001107         1793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_65958_length_1793_cov_2.271577, whole genome shotgun sequence.
ACCESSION   DAOSPL010001107 DAOSPL010000000
VERSION     DAOSPL010001107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65958_length_1793_cov_2.271577"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PLQ54_17195"
     CDS             <1..637
                     /locus_tag="PLQ54_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95054.1"
                     /translation="HSQPSPVRFELDPPVSGTVDVLFENRSVMAEHGTWTDTIEGFGT
                     RVYRISVNQPEPALAAMEPANLIANPSFEDAHNVGTPDGAYISLSGDPGATWFVDPRL
                     AAHGRQSLRLSAPADGQGLTLRLYPVTLEKGKRYRLSVWARGDRPGQRFSLTVDAVDA
                     ALGTHELATDWQQTVVEFAASEAAGERVYPRLELLTAGKAWFDLVQLCEME"
     gene            798..>1793
                     /locus_tag="PLQ54_17200"
     CDS             798..>1793
                     /locus_tag="PLQ54_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95055.1"
                     /translation="MPVRTHVRGVRGIVTVCGLAASIALSGCGSNGGGGRTDVDAVVR
                     AWAESLQKQDITTYQRLAAANYSYDGAAAATEDGDSSGLSAPAFMTDGVGTTTTEIIP
                     PIEEISPTHVRVRVRVQFVGTMANWFYGGGYVVDGGLLPTTDAANGASPPGDLGDAGG
                     EQPTSTPPNGFATPGFGGTTRPLANGSRGQAASHRGVSLRQFGEQVSYTAVVVLDLEQ
                     ITGAWFIVAQRVESSITLIGQVLSAPIIDQVLVNGSESATARPGTDVTVSGTARNVTD
                     RGVGARIGYKYGWIPIEGSAFSGTLRAPRVAGAYVAEVTATSWSYGGGWAMATRSV"
BASE COUNT          286 a          547 c          667 g          293 t
ORIGIN      
        1 gcatagccag ccgtcgccgg tgcggtttga gctggacccg ccagtctcgg gcaccgtgga
       61 cgtcctgttc gagaaccgct cggtgatggc agagcatggg acctggacgg acacgatcga
      121 gggcttcggc acccgtgtgt accggatctc cgtgaaccag cccgagcccg cactggccgc
      181 catggagccc gccaacctca ttgcgaaccc gagtttcgag gacgcgcaca acgtcggcac
      241 acccgacggc gcctacatca gcctgagcgg cgaccctggt gccacgtggt tcgtggatcc
      301 gcgtctcgcg gcccatggcc ggcagtcatt gcggctcagt gcgccggcgg atggccaggg
      361 gctcacgctt cggttgtacc cggtgacgct ggagaagggg aagcggtacc ggctgtcggt
      421 ctgggcccga ggtgaccgtc cggggcagcg cttctcgctg acggtcgacg ctgtggacgc
      481 ggcgttgggc acgcacgagc tggcgacgga ctggcagcag acagtcgtcg agttcgcggc
      541 atccgaggcg gcgggcgagc gcgtctaccc gagactggag ctgcttaccg cggggaaagc
      601 gtggttcgat ctcgtgcagc tgtgcgagat ggagtagagt ggttgtcgaa tgatgagaga
      661 cagcgtgtgg ttttcccaag acgccggact ggtgggtgcc gtcatgctgg gtgggggagg
      721 tggcccaggg ccaaccgcca cccctgttgc gaccggcttc gcggggcccg tcgcgaccta
      781 cgctccagga ggaagtgatg ccggtgcgga cacatgtgag gggcgttcgc gggatcgtca
      841 cggtgtgcgg ccttgccgcg tcgatcgcgc tgtcgggctg tggatcgaac ggcgggggag
      901 gccgcacgga cgtcgacgcg gtggtgcgag cctgggcgga gtccctacag aagcaggata
      961 tcaccactta tcaaaggttg gcggccgcca actacagcta tgacggcgcc gcggccgcaa
     1021 cagaggatgg cgattcgtcc ggcctgagtg ccccggcatt catgacggac ggcgtcggga
     1081 cgacaaccac ggagatcatt ccacccatcg aggagatatc ccccacccat gtgcgcgtcc
     1141 gtgtgcgcgt gcagttcgtc gggacgatgg ctaactggtt ctatggcggt gggtacgtcg
     1201 tggacggcgg gctgctgccg acgacggatg ccgccaatgg ggcttcgccg cccggtgact
     1261 tgggcgatgc cggaggcgag cagccgacat cgacgccgcc caatgggttc gcaacgcctg
     1321 ggttcggggg gacgacccgg ccgctggcga acggctctcg cgggcaggcc gcgagccacc
     1381 gaggggtgtc gctccgccag ttcggcgagc aggtctccta cacagccgtg gtggtactcg
     1441 acctcgagca gatcacgggt gcatggttca ttgtcgccca acgggtcgag tcctccatca
     1501 cgctgattgg gcaggtgcta tcggccccga tcatcgacca ggtgctggtg aacgggtcgg
     1561 agtccgccac ggcgcgcccc gggactgatg tgaccgtcag cggcacggcg aggaatgtca
     1621 ccgaccgagg cgttggagcc cgcatcggct acaagtacgg gtggataccg atcgagggca
     1681 gtgccttcag cgggacgctg agggctccgc gcgtggccgg agcctatgtg gctgaggtca
     1741 ccgccacgag ctggtcgtac ggcggtggct gggcgatggc gacgcgcagc gtc
//
LOCUS       DAOSPL010001108         1792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_65997_length_1792_cov_2.830167, whole genome shotgun sequence.
ACCESSION   DAOSPL010001108 DAOSPL010000000
VERSION     DAOSPL010001108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65997_length_1792_cov_2.830167"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLQ54_17205"
     CDS             complement(<1..239)
                     /locus_tag="PLQ54_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95056.1"
                     /translation="MRFGEGRFIKHMDFRMVAAALALLAIGTVLVVSTPVAEGRLARQ
                     LAYAAAGVAVLVVAIHVDYDFIRIAYYITYALTILV"
     gene            479..1447
                     /locus_tag="PLQ54_17210"
     CDS             479..1447
                     /locus_tag="PLQ54_17210"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS95057.1"
                     /translation="MPPLRVALVGAGGIGRTHLTAWKSVPGVEIVAVVDTDTARGETA
                     AGEVGARAFDAVVPMLDAVRPDAVDICTPPVAHRAPAEAALAAGAHVLCEKPLADTPG
                     DAKVIVDAAERSGRLLMTAFCHRFHPPVARLKELITEGRLGRIGMFRNRFAGPFVGVE
                     DVWFSKPEVSGGGCLMDTSVHSVDCFRFLVGEVVRAEGAGLTLNPELAPGVEDTAAMM
                     LVAESGAIGIIESSWALQGGVNVVEIYGTEGVAYVHYWDGFTSRYKTKDMAGYESLPE
                     DGDRFVGELSHFAAAATGQCPLSVTGHDGLRAVEIIYQAYASAGLR"
BASE COUNT          265 a          609 c          615 g          303 t
ORIGIN      
        1 acgagaattg tgagggcata ggtaatgtaa tacgcaatcc ggatgaagtc gtagtccacg
       61 tggatcgcga cgaccaagac cgccacaccc gccgccgcgt aggcgagttg ccgggccagg
      121 cgcccttctg ccacgggcgt gctgaccacg agaacggtcc cgatggccag cagcgcgagc
      181 gccgcggcca ccatccggaa gtccatatgc ttgatgaaac ggccttcgcc gaacctcaat
      241 gccactcacc agtgtggacc cgatctgggg cggtaggcaa ggcgcgcggc ccggcgaggg
      301 gacgcggccc tgccggtcgc gctcgattat acacggtgcc atgggcccag atgtgccatg
      361 cggtgccatg aggaagggtt cgctagcccc cgacggcgaa catctcgggc gagcctgagg
      421 ggcatcatca tgcatcgctg cctccaggcg agtctgcctg aggaggggtc cacccgagat
      481 gcccccactt cgcgttgccc tcgtcggcgc cggcgggatc ggccggacgc acctgaccgc
      541 ctggaagtcg gtccccggcg tcgagatcgt ggcggtcgtt gatacggaca ctgcgcgcgg
      601 cgagacggcc gccggagagg tcggcgcgcg ggcgttcgac gccgtggtgc cgatgctcga
      661 cgcggtcaga ccggacgcgg ttgacatctg cacaccaccg gtggctcacc gggcaccggc
      721 cgaggccgca ctggcggcgg gggcccatgt gctgtgcgag aagcccctag ctgacacccc
      781 gggcgacgcc aaggtgatcg tggacgcggc cgagaggtcc ggcaggctgc tcatgaccgc
      841 cttctgtcac cggttccacc cgcccgtggc gcggctgaag gagctcatca ccgagggccg
      901 tctgggccgc attggcatgt tccgcaaccg tttcgccggg ccgttcgtcg gtgtcgagga
      961 tgtgtggttc tcgaagcccg aggtctccgg cggcggctgc ctcatggaca ccagcgtcca
     1021 cagcgtcgac tgcttccgct ttctggtggg cgaggtcgtc cgggccgagg gggctgggct
     1081 cacgctcaac ccggagttgg cgcccggtgt cgaggacacg gcagccatga tgctcgtcgc
     1141 cgagtccggc gccatcggca tcattgagtc gagttgggca ctccagggcg gcgtgaacgt
     1201 cgtcgagatc tacggaaccg agggtgtggc ctacgttcac tattgggacg gcttcacctc
     1261 ccgctacaag accaaggaca tggccggata tgagtcccta cccgaggatg gcgaccgctt
     1321 tgtgggcgag ctgtcacact ttgccgccgc agccacgggt cagtgcccat tgagcgtcac
     1381 gggccacgat ggtctgagag ccgtcgagat catctaccaa gcctatgcct cggccggact
     1441 gcggtaggcg gcctcgcacc tgaacccggc gcgcagcgct ccgttctgat ttcgggtgcc
     1501 ggttgccatc cagcacgtgg ctcgtggccc tcaccccatc ccagctgccg gagcggatcg
     1561 ccgtgcgtcg ctggcctctg gtcatcgttc ttgcgtcgct cgcgctcgct gtcgccctcg
     1621 gcgtggggcc tgtcccctta gccggcgcct caccgctcgg tgcgccggtt gtcggtgcga
     1681 gcttcgagcc cgccagcgcc gtcggcctcc ttggcccgga ctgtgttcgg ccgggcgagc
     1741 gcgtcacact ccgctgtgat cccgcgcccc agaccagtgg gcccatcctg cg
//
LOCUS       DAOSPL010001109         1790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_66101_length_1790_cov_3.106052, whole genome shotgun sequence.
ACCESSION   DAOSPL010001109 DAOSPL010000000
VERSION     DAOSPL010001109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66101_length_1790_cov_3.106052"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PLQ54_17215"
     CDS             <1..687
                     /locus_tag="PLQ54_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95058.1"
                     /translation="AREKGLEVGVDFSLRHYWAGAKLPLEQGEIGDLLSCTFAGPHAL
                     GLGRRPSWYFEPGMHGGIINDLMGHGLDYVGWVTGQRCVEVLSATRVTGLLREHPGFE
                     MLGEAHFRLADGLTAFGHVDYLAPEGHAGGWWLAFTGSEGDALLSEASGLRLRRTGAA
                     ETHIAAAELRSASPHPFVDFVGLLTDGTPPLRTTAEALDCSLASLVAQRLAASGEGRG
                     PVDGTEVSGK"
     gene            698..1213
                     /locus_tag="PLQ54_17220"
     CDS             698..1213
                     /locus_tag="PLQ54_17220"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOS95059.1"
                     /translation="MVRIETRRLVIRNYTADDWRDVQRIAMDYSASEFAKYDHPWPTD
                     DEGVQGATNWFASGDGYLAVCLRSTGECIGLLALHPRDDADPKVYNLGYIFHSDYHGH
                     GYATEACRAGLAHAFGKLGVERIATGTAVANLASCRLLERLRLRRPGERSEDLWTLTR
                     DEWLAQQGEPD"
     gene            complement(1271..>1790)
                     /locus_tag="PLQ54_17225"
     CDS             complement(1271..>1790)
                     /locus_tag="PLQ54_17225"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOS95060.1"
                     /translation="AALYCNARSKAEGLKPCYDEETALCDFGASGYRLPTEAEWEYAC
                     RAGTDTEYPFGKIGWNLSAYAWFEANAEGMTHPVGQRRPNRWGLFDMLGNVAEWCNDI
                     YSETYYAGSPDRNPTGPADGERYVIRGGAWNSPAAHCRSACRLTAGPGFQDACFRRDD
                     IGFRCVRAVPSR"
BASE COUNT          267 a          612 c          635 g          276 t
ORIGIN      
        1 gcccgggaga agggcctgga ggtcggagtc gacttcagcc tgcgccacta ctgggctggg
       61 gcgaagctgc ccctcgagca gggcgagatc ggggacctgc tgagctgcac cttcgccggg
      121 ccccacgcgc tcggcctggg ccggcggccg agctggtact tcgagcccgg gatgcacggc
      181 ggcatcatca atgacctcat gggccacggg ctcgactacg tgggctgggt caccgggcaa
      241 cggtgcgtag aggtcctctc ggcgacgcgc gtgaccgggc tgctccgcga gcacccgggc
      301 ttcgagatgc tcggcgaagc ccacttccgg ctcgccgacg ggctgacggc gttcggccac
      361 gtcgactacc tcgcgcccga gggccatgcg gggggctggt ggctggcctt cacgggcagt
      421 gagggcgatg ctctgctcag cgaagcaagc ggcctccgcc tgagacgcac aggggctgcc
      481 gagacacaca tcgccgctgc cgagctccgc tccgcgtctc cacatccgtt cgtggacttc
      541 gttggcttgc tgacagacgg caccccacct ctgaggacga cggcggaggc gctggactgc
      601 tcgctcgcgt cgctggtcgc tcagcggttg gcggcgtcgg gcgaggggcg ggggccggtg
      661 gacggaactg aggtatccgg gaagtgaggg ggcttccatg gtccgcatcg agacacggcg
      721 gctcgtcata cgcaactaca ccgccgatga ctggcgcgac gtgcagagga ttgccatgga
      781 ctacagcgcc tcggagtttg ccaagtacga ccatccgtgg ccgacggacg acgagggcgt
      841 ccagggagcc acgaactggt ttgccagcgg tgacggctat ctggccgtat gcctccgatc
      901 gaccggcgag tgcatcggcc tgcttgcgct gcatccgcgg gacgacgccg acccgaaggt
      961 gtacaacctc ggctacatct tccactccga ctaccatggg cacggctacg ccaccgaggc
     1021 gtgccgcgcc ggcctggcac acgcattcgg gaagctcggc gtggagcgca tcgccaccgg
     1081 cacggctgtg gccaacctgg cgtcttgccg gctgctcgag aggctgcgcc tccgtcgccc
     1141 gggcgagcgt tccgaagacc tgtggaccct caccagggac gagtggctcg cccagcaggg
     1201 cgagcccgac tgacgcgcag cggccacgtc ttcgatcctt ggccgaccgc ccggttgctc
     1261 ccgtgttacc ctagcggctc ggcaccgccc tcacgcaccg gaaaccgatg tcgtcgcgcc
     1321 ggaagcacgc gtcctggaac ccggggcccg cggtgaggcg gcaggcggag cggcagtgcg
     1381 cggccggcga gttccaggcc ccgccgcgga tgacgtaacg ctcgccgtcc gcaggcccag
     1441 tggggttgcg gtcagggctc ccggcgtagt acgtctcgct gtagatgtcg ttacaccact
     1501 cggcgacatt cccgagcatg tcgaagaggc cccagcggtt ggggcggcgc tggcccaccg
     1561 ggtgagtcat gccttcggcg ttcgcctcga accaggcgta ggccgacagg ttccagccga
     1621 tcttcccgaa ggggtactcg gtgtcagtgc ccgcgcggca ggcatactcc cactcggcct
     1681 cggtgggcaa gcggtagcca cttgccccga agtcgcacag ggcggtctcc tcgtcgtagc
     1741 agggcttcag cccctcggcc ttcgaccgcg cgttgcagta gagggcagcg
//
LOCUS       DAOSPL010001110         1788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_66192_length_1788_cov_2.821119, whole genome shotgun sequence.
ACCESSION   DAOSPL010001110 DAOSPL010000000
VERSION     DAOSPL010001110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66192_length_1788_cov_2.821119"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PLQ54_17230"
     CDS             <1..570
                     /locus_tag="PLQ54_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95061.1"
                     /translation="RYAHASRTPEEAHCGQCGAVHEWLAVRAHWRSHPMCTCGARLES
                     TRGTLACSACGRTWSAGQFAAILARRRTGPCPTCGARIARMPDVVRCGACGREFAWSR
                     FRRAWQGRGLMTGAGVPACERFVSRWSRACGPMEQMLAIDMFLHELHYGPLAPLFVDG
                     GRHAVLAMLDEFAGVGPSGPPTPAPDTAR"
     gene            complement(542..1639)
                     /locus_tag="PLQ54_17235"
     CDS             complement(542..1639)
                     /locus_tag="PLQ54_17235"
                     /inference="COORDINATES: protein motif:HMM:NF024381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3863 domain-containing protein"
                     /protein_id="HOS95062.1"
                     /translation="MTWAFSWRALQDPRPNYQAIRELVAMFHVEHGDDVTFIPGGFFA
                     NAYGTREQISLDLHEGLEMAAAIVGGGYRPPAVVAGFLAAANQRHLADVEDVHVCQGN
                     IWSQYAIDNQDGDGSICYPYFPSAEHFCKPAQGPGDFIDSVCLDGWTTDFLAARREGF
                     RDGFNSRMGVGPIETLGAYGTEVGVRQMVATTAAHFDAGHDLNGFAWVTNCWEVSLVT
                     SIGQLDGLERWLHQVRRRWPGAQCPTLGEFGLAWRRANPDNSRLNYRFVQRGTGIGGS
                     DADLEIRWFMNQAFRLALLRSWRDNGPEMVIDFTRYDLPAREPQEATRRWSLMGQINQ
                     KQTRPQDKPVLLRELPPEDQERIIARYPELG"
BASE COUNT          253 a          629 c          596 g          310 t
ORIGIN      
        1 cggtacgctc acgcatcgcg cacgcctgag gaggcgcact gcgggcagtg cggcgccgtg
       61 cacgagtggc tcgcagtgcg agcacactgg cgcagccacc ccatgtgcac atgcggggcg
      121 cgtctggagt ccacccgcgg cacgctggcc tgctcagcat gtggccgcac ctggtccgcg
      181 ggccagttcg ccgctatact cgcccgtcgg cgcaccggtc cttgtcccac ctgcggggcg
      241 cgcatcgcgc gcatgccgga cgtggtgcgc tgcggcgcct gtggccgcga gttcgcctgg
      301 tcgcgcttcc ggcgggcctg gcagggccgc ggcctcatga ccggcgccgg tgtgcccgcc
      361 tgcgagcggt tcgtcagccg gtggtcccga gcgtgcgggc cgatggagca gatgctcgcc
      421 atcgacatgt ttctgcacga gctgcactat gggccgcttg cgcccctctt cgtcgacggg
      481 ggccgccacg ccgtgctggc gatgctcgac gagtttgcgg gggtggggcc ctccggtccg
      541 cctaccccag ctccggatac cgcgcgatga tgcgttcctg gtcctcggga ggcagctcgc
      601 gtagcaacac gggcttgtcc tgaggccgtg tctgcttctg gttgatctgc cccatcaggc
      661 tccaccgtcg cgttgcctcc tggggttccc gggccggaag gtcgtaccgc gtgaagtcga
      721 tgaccatctc gggcccgttg tcgcgccagc tcctcagcag cgccaggcgg aacgcctggt
      781 tcatgaacca ccggatctcg aggtctgcgt cggatccacc gatacccgtg cccctctgca
      841 cgaagcggta gttcaggcgc gagttgtccg gattcgcgcg gcgccatgcc aggccgaact
      901 cgcccagtgt tgggcactgg gcaccgggcc agcgccgacg tacctggtgg agccatctct
      961 cgaggccgtc gagttgcccg atcgacgtga cgaggctgac ttcccagcag ttggtcaccc
     1021 acgcgaagcc gttcaggtcg tgtccagcgt cgaagtgcgc ggcggtggta gcgaccatct
     1081 gtcggacgcc cacctcagtc ccgtaagcgc ccagggtctc gatcggccca acccccatgc
     1141 ggctgttgaa gccatcgcgg aacccctcgc ggcgggccgc caggaagtcc gtggtccagc
     1201 cgtcgaggca cacgctatcg atgaagtccc ccggtccctg ggcgggcttg cagaagtgct
     1261 ccgccgatgg gaagtacggg tagcagatgg agccatcgcc gtcctggtta tcgatcgcat
     1321 actggctcca gatattgccc tggcacacat ggacgtcctc gacatcggcc aggtgccgct
     1381 ggttggccgc ggcgaggaaa ccggctacca cggccggcgg cctgtaaccg ccaccgacga
     1441 tggcggctgc catctcgagg ccctcgtgga ggtcgaggct gatctgctca cgggtgccgt
     1501 aggcgttcgc gaagaaacca ccgggaatga aagtgacgtc atccccgtgt tccacgtgga
     1561 acatcgccac gagctcgcgg atcgcctggt agttggggcg tggatcctgc agggcccgcc
     1621 aactgaacgc ccaggtcatc cggccgcccg gccaccctcg gccgaacgcc tcgcgcagct
     1681 cgttcaactt ctgaggcttc tctgcctcca gatcccactt gttgaccgcg accaccaccg
     1741 cccgcccctc gcgttcggcg aggtcggcga tgcgcaggtc ctgactct
//
LOCUS       DAOSPL010001111         1786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_66301_length_1786_cov_3.711150, whole genome shotgun sequence.
ACCESSION   DAOSPL010001111 DAOSPL010000000
VERSION     DAOSPL010001111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66301_length_1786_cov_3.711150"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLQ54_17240"
     CDS             complement(<1..202)
                     /locus_tag="PLQ54_17240"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOS95063.1"
                     /translation="MKAWVFYEPGKHQFEAVADPAPAADQVMVQVKACGICGSDVAYH
                     YGMSSLETPNGKGPLILGHEYTG"
     gene            complement(403..1305)
                     /locus_tag="PLQ54_17245"
     CDS             complement(403..1305)
                     /locus_tag="PLQ54_17245"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS95064.1"
                     /translation="MAKKKLAIGSWAYCFGPYANHPHPLDEVVRKLAELQFDGIEVCG
                     FPPHVSPEAYTTPASRAEAKRMFADAGLEVCGYAADLTPTPPPVVPDVAPYVERFKVC
                     AQIAAEIGSPSIRVDTVAPPVLPEGVSYDTAWKRTVEKWNAAAGVAQDMGLGLVWEFE
                     PGFLFNKPSEIVKMISDVDHPNFMILFDTCHAQMVAVNGSRQLPPKETLPGGIVELAG
                     MLKGRIGHIHLIDSDNSLHDNDTSTHAPFGQGVIDFDTVIPAIIAAGYDSEWWSIDLC
                     FWPEAWEVTEAAKRFLQPYIEKYS"
BASE COUNT          313 a          576 c          577 g          320 t
ORIGIN      
        1 gccccgtgta ctcgtgcccg aggatgagcg gacccttacc gttcggggtt tcgagcgatg
       61 acattccata gtgataggcc acatccgacc cgcagatccc gcaggccttg acctgaacca
      121 tcacttggtc tgccgccggc gcggggtcag ccaccgcctc aaactgatgc ttccctggct
      181 cgtagaacac ccatgccttc atgcgcgatg cgcctcctgt gggttgttga gagacccagc
      241 actatcgggc gacacggccg accggatggc gcccttcccg cttcgcgggc cccttccatc
      301 cggcccctcc tcggcctctc ggaaggtgtt gggaggggcc gaacagggac catccaccgg
      361 atggtgggac cctaaccggc ccgtctcacg ttcggcctcg ctctagctgt acttctcgat
      421 gtacggctgg aggaaccgct tggcggcctc agtgacctcc caggcctctg gccagaagca
      481 caggtcgata ctccaccatt cgctgtcgta accggccgcg atgatggccg ggatgacggt
      541 gtcgaagtcg atgactccct ggccgaatgg tgcgtgagtg ctcgtgtcat tgtcatgcag
      601 cgagttgtcc gagtcgatca agtggatatg accaatcctg cccttgagca tcccggccag
      661 ctcgacgatg ccgccgggaa gtgtctcctt gggcgggagc tgccgggagc cattgaccgc
      721 caccatctgg gcgtggcacg tgtcaaacag gatcatgaag ttggggtgat ccacgtcgga
      781 gatcatcttc acaatctcac tgggcttgtt gaagaggaac cccggctcga actcccatac
      841 gagcccgaga cccatgtcct gcgccacgcc agcggcggcg ttccacttct ccaccgtgcg
      901 tttccatgcg gtgtcgtagc tgacgccctc aggcagaact ggaggggcca cagtgtccac
      961 acggatgctt gggctgccga tctcggccgc gatctgcgcg cagaccttga accgttccac
     1021 atagggcgcc acatcgggca cgacaggagg tggggtgggg gtcagatcag cggcgtaccc
     1081 acacacctcg agcccggcat cggcgaacat gcgcttggcc tcggcacggc tcgccggggt
     1141 ggtgtacgcc tcgggcgaca cgtgcggcgg gaacccgcag acctcgatgc catcgaactg
     1201 cagctcggcg agcttccgga ccacctcgtc cagcgggtgg ggatggttcg catacggccc
     1261 gaagcagtat gcccacgagc cgatggcgag cttcttcttc gccatggttg gcctcctcac
     1321 gcggatcccg ggcgtgcgcg gcgcgggcga gcccgtcggc cccgggagac ctgagggagt
     1381 tcgccgcggg tgcccgagtt cctgcgtcac aatggcgtca gccgtaatgc ggcaatggtt
     1441 gccggaaata ggtctggcga tgggccgtaa catgccaaac gctggccata gtgacggaaa
     1501 cagacggaaa acacggcggt ccgcacggcc caaacgggcc gcgactcgcc cggcggaccg
     1561 gcgattgcgg cgtacgttgc cgatgtgcta ccctacagct tccgccccct acggtccgcc
     1621 gctggaccgg gcgggatgct ttgaggtagt ggggcatcag acagcggatc tgagaaagga
     1681 acacaccatg tgcggaatcg tcggctatat cggctcgaaa cgcgcgacgg acatcctggt
     1741 tggcggcctc aagaggctcg agtaccgtgg gtatgactcg gcgggc
//
LOCUS       DAOSPL010001112         1785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_66368_length_1785_cov_2.388439, whole genome shotgun sequence.
ACCESSION   DAOSPL010001112 DAOSPL010000000
VERSION     DAOSPL010001112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66368_length_1785_cov_2.388439"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1785
                     /locus_tag="PLQ54_17250"
     CDS             <1..>1785
                     /locus_tag="PLQ54_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95065.1"
                     /translation="GSESFAQWNAPAFEIEPGEHVIRVRATENGVALDALRAWRSGEG
                     VCDVRLTPLPEAAHAGVTLPGYYGVFYDNESPEFRLTLSSLTDGATTVGGTYELRNYM
                     GEVVDGGTLEPVTLRPGSPRDVNLRFTPTETGRFHLTVHVNTEQGPIARTFDFARLPK
                     LEHPRLIYRADQKEAIRRRIAEHPRLFARYTAWLRRKSAETGGRFPERFMPPGLTAAD
                     LVTARPEGMSADDAGNAYAWRMYELGWRILACQFASEFLEPEGERVLGQRVDELLGKG
                     KTDYYCEFHHHGPFFPGAVAGAWDMAPASTRNSTKLKETFEDRLGDMNVYPWTLVTLE
                     EPITPQTRATVAKIMQWENNLENYFAVHQGTRGGSWWQNPWTGCNCPVHGQVLGFLFS
                     SNLLGETRLFEKQLFTGFLTFTRLVDPLSDRRGVLPATRGPQGEPWRFISCALPRHPL
                     EATLYQWDEWYRKLDGDLPQPEDEAVDRLMELEGMELDGQLVGGSSRFVTGVFAPIAL
                     ALGWYDPAQPRVERSELPETTLFDVEGWATMRSDWGPDATEVSFISGVRDHTYRHQPN
                     HFMIVRGGQFLVGTPSTWSDDGNCNPAW"
BASE COUNT          300 a          590 c          613 g          282 t
ORIGIN      
        1 acgggtcgga gagcttcgcg cagtggaacg cgccggcgtt cgagatcgag ccgggcgagc
       61 acgtgatccg cgtgcgcgcc accgagaatg gcgtggcgct cgatgccctg cgggcgtggc
      121 gctcggggga gggggtgtgc gatgttcgcc tcacgcctct ccccgaggcg gcccatgcgg
      181 gcgtcactct ccccggctac tacggcgtct tctacgacaa cgagagcccc gagttccggc
      241 tgacgctgag cagtctgacc gatggggcca cgaccgtcgg cggcacgtat gagctacgca
      301 actatatggg cgaggtggtc gacggtggca cgctcgaacc ggtgaccttg cggccgggca
      361 gtccacggga tgtgaacctg cgcttcacgc ccacggagac gggacggttc catctgacgg
      421 tgcacgtgaa caccgagcag ggcccgatcg cgaggacatt cgacttcgcg cgcctcccga
      481 agctcgaaca cccgcggctc atctaccgag cggaccagaa ggaggccatt cggcggcgca
      541 tcgccgagca cccgcgcctg ttcgcccgtt acaccgcatg gctgcggcgc aagtcggccg
      601 agacgggcgg acggttcccg gagcggttca tgccaccggg actcactgcg gccgatctcg
      661 tcacggcgcg acccgaaggc atgtccgccg atgacgcggg gaacgcctac gcgtggcgca
      721 tgtacgagct gggttggcgg atcctggcat gtcagttcgc ctcggagttc ctcgagccag
      781 agggcgagcg cgtcctgggc caacgcgtgg acgagctcct gggcaagggg aagacggact
      841 actactgcga gttccaccac catgggccct tcttcccggg ggccgtcgcg ggggcgtggg
      901 acatggcccc cgcgagtacc cggaacagca ccaagctcaa ggagaccttc gaggaccggc
      961 tgggcgacat gaacgtctat ccgtggacac tggtcaccct ggaggagccc atcacgcccc
     1021 agacacgggc cacggttgcc aagatcatgc agtgggagaa caacctcgag aactacttcg
     1081 ccgtgcacca gggcacccgc ggcgggtcat ggtggcagaa tccgtggacc gggtgcaact
     1141 gcccggtgca tggccaggtg ctcggcttcc tgttctcgag caacctgctc ggcgagaccc
     1201 gcctctttga gaagcagctg ttcacgggct tcctgacctt cacccggctg gtggatccgc
     1261 tctccgaccg acgcggtgtg ctcccagcca cacgggggcc tcagggggag ccgtggcgct
     1321 tcatctcgtg tgcgctgccc aggcatccgc tcgaggccac tctctaccag tgggatgaat
     1381 ggtatcggaa gctcgatggc gacctgccgc agcccgagga cgaggccgtc gaccgcctga
     1441 tggagctcga aggtatggag ctcgacgggc aactggtcgg cggcagcagc cgtttcgtga
     1501 ccggtgtctt cgctcccatc gcactggccc tcggctggta tgacccggcc cagccccgcg
     1561 tggagcgcag cgaactgccc gagacgaccc tgttcgatgt cgagggctgg gccaccatgc
     1621 ggtcggattg gggacccgat gccaccgagg tgtcattcat atcgggcgtc cgggatcaca
     1681 cataccggca tcagccgaac cacttcatga tcgtccgggg cgggcagttc ctggtgggca
     1741 cgccctcgac ctggagcgac gacggcaact gcaacccggc gtggg
//
LOCUS       DAOSPL010001113         1784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_66397_length_1784_cov_4.282244, whole genome shotgun sequence.
ACCESSION   DAOSPL010001113 DAOSPL010000000
VERSION     DAOSPL010001113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66397_length_1784_cov_4.282244"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="PLQ54_17255"
     CDS             <1..1144
                     /locus_tag="PLQ54_17255"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS95066.1"
                     /translation="SDADSRRMGPVADKVARISGTAPRQLQDFRHILDIGHVDALISA
                     TPDHWHALATIWACQAGKDVYVEKPASHNIWEGRKMVEAARKYRRVVQVGAQNRSAPE
                     VMAGVEYLRSGKLGSVHFVRVLNMKQRDPIGKKADEPVPSGVNYDLWCGPAPMKPFNP
                     NRFHYSWHWFWDFSGGDIINDGVHQIDIARWLIDKPYPRAVSCTGGKYAYDDDQETPD
                     TQVAHWEYDDMTLSFNLTLWTPYMQKTAWEVRDLDQFPDWRFCATRIEVYGTKGMMMM
                     GRHGEGWQVYGIDGKVVDSHFGRHPHGPHLENFFACIRDRKTPSGDIEELHRSTFLCQ
                     TANIAYRVGGRRLEIDPEGECFIGADDANVLVKRRGRAPYQVPERV"
     gene            1295..>1784
                     /locus_tag="PLQ54_17260"
     CDS             1295..>1784
                     /locus_tag="PLQ54_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95067.1"
                     /translation="MRFLAAGSSVLILLVACAAVAQDITPPVVTEVEAKVLAPTTGNY
                     EPSQAITATTQHGMKYNSTAHGFPPYAYEIHWRQYPSWASKSARYGTVTANDFHGSDG
                     TDWNHITPNPTYPWQVPGRTGITHVFTAETYARAACRTKPPGVPGGWVSRRSRGTPAW
                     SFG"
BASE COUNT          342 a          614 c          548 g          280 t
ORIGIN      
        1 cagcgatgcc gactcgcgcc gcatgggccc ggtggcggac aaggtcgccc ggatctccgg
       61 caccgccccg aggcaactcc aggacttccg ccacatcctc gatatcggcc acgtggatgc
      121 cctcatcagc gccacgcccg accactggca cgccctcgcg accatttggg cctgccaggc
      181 gggcaaggat gtctacgtcg agaagccggc cagccacaac atctgggagg gccgcaagat
      241 ggtcgaggcg gcccggaagt accgccgcgt cgtccaggtg ggcgcccaga accgcagcgc
      301 tccggaagtc atggccggtg tcgagtacct gcgctccggc aagctcggca gcgtgcactt
      361 cgtccgcgtg ctcaacatga agcagcgcga tcccatcggc aagaaggccg atgagccggt
      421 cccctccggt gtcaactacg acctgtggtg cggcccggcc cccatgaagc ccttcaaccc
      481 gaaccggttc cactactcct ggcactggtt ctgggacttc tccggcggcg acatcatcaa
      541 cgatggcgtg caccagattg acatcgcccg atggctcatc gacaagccct acccgcgcgc
      601 ggtctcatgc accggtggga agtacgccta cgacgacgac caggagaccc ccgacaccca
      661 ggtggcccac tgggagtacg acgacatgac gctgagcttc aacctgacgc tctggacgcc
      721 gtacatgcag aagacggcct gggaggtccg cgatctcgac cagttccccg actggcggtt
      781 ctgcgcgacc cgcatcgagg tctacggcac caagggcatg atgatgatgg gccgacacgg
      841 cgaaggctgg caggtctacg ggatcgatgg caaggtcgtc gactcccact tcggccggca
      901 cccgcacggg ccgcacctcg agaacttctt cgcctgcatc cgcgatcgga agactcccag
      961 cggcgatatc gaggagcttc atcggagcac attcctgtgc cagacggcga acatcgccta
     1021 ccgcgtcggc ggccgccggc tcgagatcga ccccgagggt gagtgcttca tcggtgccga
     1081 cgacgccaac gtgctggtca agcgccgggg ccgcgcaccc taccaggtgc cggagcgggt
     1141 gtgagacacc ccgaatgcga gcggcctgct atacagaagt atttcaatag aaaggacgat
     1201 actttcccct gtctgtcgaa caggtactct aacattcgcc actcggatag gctgcgggca
     1261 ctgccggggc caatgcgtcc ggggggaatg cggtatgcgg ttcctcgctg cgggatcgtc
     1321 ggtcctgata ctccttgtgg cctgcgctgc cgtcgctcag gacattacgc cgccggtcgt
     1381 tacggaggtc gaagccaagg tacttgcccc cacgaccggg aactacgagc caagtcaggc
     1441 catcaccgcg accacgcagc acggaatgaa gtacaactcc acggcccacg gctttccgcc
     1501 ctacgcctac gagatccact ggcgtcagta cccctcgtgg gcctcgaaaa gtgctcgcta
     1561 cggcaccgtg actgcgaacg acttccacgg tagcgatggc acagactgga accacatcac
     1621 gcccaatcca acctatccgt ggcaggtgcc cgggagaacg ggcatcaccc acgtcttcac
     1681 agcagagact tacgcccgtg cggcgtgccg gaccaagcct ccgggggtac cgggcggctg
     1741 ggtctcgcga aggtcgcgcg gaacgcccgc ctggtctttc ggag
//
LOCUS       DAOSPL010001114         1784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_66400_length_1784_cov_3.685946, whole genome shotgun sequence.
ACCESSION   DAOSPL010001114 DAOSPL010000000
VERSION     DAOSPL010001114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66400_length_1784_cov_3.685946"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1073
                     /locus_tag="PLQ54_17265"
     CDS             120..1073
                     /locus_tag="PLQ54_17265"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOS95068.1"
                     /translation="MSTSAAPGPNEGVGLAAIQMPLRWFPSALEFCETVDRLAARARS
                     LGADVVVYPEDVGLLIAFGDEAAGLQLVTDVRTAVRALFSKHQAQAMEIMQRHPVGPA
                     RALLLLRAQTTRERYVSMFSDVARRHSLYVVAGSAPLTRVEDGDDPSAVYNMACTFGP
                     DGALLGVQCKVNLIDIERLVGLDLTAGRPEDYRAIRTPWGGIGVSICADTFDRELIDR
                     LVADGAKVIADPRANPKPWTADEAAENRKSAWSRCQERSLYAVQAFGVGQLVGIPFQG
                     RSAICAPCHLTPDGSGVLAQARTHSAEDVVVARVRLEDLGQ"
     gene            1097..1738
                     /locus_tag="PLQ54_17270"
     CDS             1097..1738
                     /locus_tag="PLQ54_17270"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOS95069.1"
                     /translation="MRLLCLGLVVGLAVGLTADTVGAVTLDFEDLWDGTKDNDPIPDG
                     YAGFDWSDYAYWESVEYISSWLPNGFVNTWEGDVGIFTAYSNPISMSGPVFDFNGARV
                     GAAWNLGQEVTFEGWLDADLLYSQTLTLGTQGQEYVFDFIGINTLWIRPDVTTGTKDP
                     AMHSAGAGHHIAMDNFVINETRVPTPEPGTLALLGLVGIPAAAKSLRRRWCRN"
BASE COUNT          286 a          541 c          609 g          348 t
ORIGIN      
        1 catcctgggc cgcgcccggg acttgacatg cccttggcaa cgtgtgtcta cttctacatc
       61 gggtgcggcg agtcccgttg ccccaacgag ttgggtctca ccgaggatgt ggccgatcga
      121 tgagcacgtc cgctgcgccc ggcccgaacg agggtgtggg actcgcagcc atccagatgc
      181 cgctccggtg gttccctagt gccctcgagt tctgtgagac cgtcgaccgg cttgccgcac
      241 gggccagaag tcttggcgcc gatgtggtgg tctaccccga ggacgtgggc ctcctcatcg
      301 ccttcggcga cgaggcggcg ggccttcagc ttgtcacgga tgtccgcacg gcagtccgcg
      361 ccttgttctc caaacaccag gcccaggcca tggagatcat gcagcggcat cctgttgggc
      421 cggcccgggc cctgctgctg ttgcgggcgc agaccacgcg cgagcgctat gtctcgatgt
      481 tctctgacgt cgcccgtagg cactcgctct atgtggtcgc gggtagtgcg ccgttgactc
      541 gtgtcgagga tggcgacgat ccctcagcgg tgtacaacat ggcatgcacc ttcggaccgg
      601 atggggcgct gcttggggtt cagtgcaagg tgaacctgat cgacatcgaa cggctcgtcg
      661 gtcttgacct gacggccggc cgtcccgaag actaccgtgc catccggacg ccctggggcg
      721 gcattggggt ctcgatctgc gccgacactt ttgaccgaga gctcatcgac aggttggttg
      781 ccgacggggc aaaggtcatc gcggatccca gggcgaatcc caagccatgg accgccgatg
      841 aggcggcgga gaaccgcaag tccgcctgga gccgttgcca ggagcgatcg ctctatgccg
      901 ttcaggcatt tggtgtcggt cagctggtcg gcatcccgtt ccaggggcgt tcggcgattt
      961 gtgcgccatg ccatctgact ccggacggat cgggcgttct cgctcaggca cggacccata
     1021 gtgccgagga tgtggtcgtg gctcgggtgc ggctcgagga cctgggccag tagactacgg
     1081 gaacgggggg atcacggtga gactgctctg cctagggttg gttgtggggc tagccgtggg
     1141 actgacggcg gacaccgtgg gtgcggtcac gctggacttc gaggacctgt gggacgggac
     1201 caaggacaac gaccccatac ccgacggcta cgcggggttc gactggtcgg actatgccta
     1261 ctgggagtcg gtcgagtaca tcagctcgtg gctgcccaat ggctttgtga atacgtggga
     1321 aggcgacgtg ggaatcttca ccgcgtactc gaacccgatc tccatgagtg gccccgtgtt
     1381 cgatttcaat ggcgcccgtg tgggcgccgc gtggaacctg gggcaggagg tcactttcga
     1441 gggctggctc gatgcagact tgctgtattc acagaccctc accctcggaa ctcagggtca
     1501 ggagtacgtc tttgacttca tcgggatcaa cacgctgtgg atccggcccg atgtgaccac
     1561 cggcaccaag gatccggcga tgcattcggc cggcgcgggc caccacattg ccatggacaa
     1621 cttcgtcatc aatgagacgc gggtgccaac gcccgaaccg gggacgttgg cgcttctggg
     1681 tctggtcggc attccggcgg ctgccaagtc gcttcgccgc cggtggtgcc ggaactagac
     1741 cggtggagtc tggctgagca ccaggtccgc cgcacatcgt ggtg
//
LOCUS       DAOSPL010001115         1784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_66425_length_1784_cov_2.549451, whole genome shotgun sequence.
ACCESSION   DAOSPL010001115 DAOSPL010000000
VERSION     DAOSPL010001115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66425_length_1784_cov_2.549451"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..393)
                     /locus_tag="PLQ54_17275"
     CDS             complement(43..393)
                     /locus_tag="PLQ54_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95070.1"
                     /translation="MEGLATAESRLAGITHVCLGLGDTRVVDGAGAWTAFLAAGELVH
                     RTDGVDDPMRAEAMVGNSPESDVAGAQAVGRVAVWLNREGLPRERGPEPDAEIRSLKE
                     LPRLLEAGGAAGPN"
     gene            complement(381..1391)
                     /locus_tag="PLQ54_17280"
     CDS             complement(381..1391)
                     /locus_tag="PLQ54_17280"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95071.1"
                     /translation="MVAGTYPRLFEPLALRSRTAPNRIVMPPMVTNMGVTSEPARAYY
                     AERARGGVGTVIVEAVQVRLFAAEIGPALMRPLARCIQDAGSLAVVQLFVQDRVSPDA
                     DRLGPSALDGAREATVEELRALQRQLARAAVVCEEAGFDGVEIHGAHGFFLNRFFAPE
                     WNQRTDGYGGSLENRMRMGLECVRAVMEAKGPELLVFYRHTPAWGGYGIDESIAFAAQ
                     LVAAGVDVLDISPSLGPNGEHAHYAGLTRQHTTVPVIAVNGMNDPARADAALAEGRCD
                     LVAIGRGLLADPMWPTKVRQGREAEIVRCTECNEKCFGNLGRGEPISCVENPRVGLEW
                     RD"
BASE COUNT          276 a          632 c          623 g          253 t
ORIGIN      
        1 gaccgatcca cggatgcatc gtgcgcccgc cccctccgga ccctagttcg gcccggccgc
       61 gccgccggcc tcgaggaggc ggggcagctc cttcaggctg cggatctcgg cgtccggctc
      121 ggggccccgc tcccgcggga gtccctcgcg gttcagccac acggcgaccc taccgactgc
      181 ctgggcaccc gcgacatcgc tctccggtga gttgccgacc atcgcctccg cccgcatggg
      241 atcgtcgacg ccgtcggtcc ggtggaccag ctcccctgcc gcgagaaagg ccgtccaggc
      301 gccggctcca tcgacgaccc gcgtgtcgcc caggccgagg cacacgtggg tgatcccggc
      361 gagccgcgac tcggccgtgg ctagtccctc cattcgagcc cgacgcgtgg gttctcgaca
      421 cacgagatgg gctcaccgcg tcccagattg ccgaagcact tctcattaca ttcggtgcag
      481 cggacgatct cggcctcccg gccctgccgc accttggtgg gccacatggg gtcggcgaga
      541 agacctcgcc cgatggcgac caggtcgcac cggccctcgg ccagcgccgc atcggcccgg
      601 gcagggtcgt tcatgccgtt gacggcgatg acgggcacgg tggtgtgctg gcgggtcagc
      661 cccgcatagt gagcgtgctc cccgttgggg cccaggctgg gcgaaatgtc gagcacatcc
      721 acacccgcgg cgaccagctg ggcggcgaag gcgatgctct cgtcgatgcc atagccgccc
      781 catgccggcg tgtggcggta gaagacgagg agctccggcc ccttggcctc catcacggcc
      841 cggacacact cgagccccat gcgcatgcgg ttctcgaggg aacctccgta cccgtccgtc
      901 cgctggttcc actcgggggc gaagaaccgg ttgaggaaaa acccatgagc gccgtggatc
      961 tcgacaccgt cgaagccggc ctcctcgcac acgacggcgg cgcgcgccag ctggcgctgg
     1021 agcgcccgaa gctcctcgac cgtggcctcg cgagccccgt cgagcgcgct cggaccgagc
     1081 cggtcggcgt cgggcgagac ccggtcctga acgaagagct gaaccacggc gagcgacccc
     1141 gcatcctgga tgcaccgggc cagtggccgc atgagggcgg gcccaatctc ggcggcgaac
     1201 agccgaacct gcacggcctc gacgatgacc gtgcccacac cgccccgggc ccgctcggcg
     1261 tagtaggcgc gggctggctc gctggtcacg cccatgttgg tcaccatggg cggcatgacg
     1321 atgcggttgg gtgccgtgcg agaccgcagg gcgaggggct caaacagccg gggatacgtg
     1381 ccagccacca tcagtcacct cgcaacgcgc cgacgcgctg gccgagcgaa tatcgcgccg
     1441 gacaaggagg tacgccgggt tggccctaag aagaaagggc tgcgcggtgc aatgcgggcg
     1501 catgatacca cctgctttcg cggcaggtcg accgaggaga tgcgccaaag ccgggtgcgg
     1561 gggcttgtac ggcgcgcggg cctatggtac agaacgagac ccgagcccca tgggcgcgac
     1621 cgttcggggc catgggctgc ctgctcctgg gcgcggcgct cggtgccgcg aagccaggtt
     1681 cgtgttgcga gcctgtagga tgggtgagcg atggaagcgc agctaccggc ggtcagagcg
     1741 atgtatagtc ccgattcgga cgacgccctc gtcgagcgtg ccct
//
LOCUS       DAOSPL010001116         1784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_66426_length_1784_cov_2.533835, whole genome shotgun sequence.
ACCESSION   DAOSPL010001116 DAOSPL010000000
VERSION     DAOSPL010001116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66426_length_1784_cov_2.533835"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..929)
                     /locus_tag="PLQ54_17285"
     CDS             complement(42..929)
                     /locus_tag="PLQ54_17285"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOS95072.1"
                     /translation="MHQADENAGLDLHTAALKGRVGVVRRLLSAGADPNRRDQEGMAP
                     LHAAARMGHVDVVALLIDAGAALDPMGPRVDLAAPILRADDQGNVTQAQVWVPHGAMT
                     PLHNAAFAGHASVARLLLERGAAVDPRDGGGGTPLHWAATQGHAETAAVLVKHGAEVD
                     AVDAFDQSPLVKAAHKGHVEVARVLLAAGADPDLTEPKTGFTPVHWASYFEHAEVLDV
                     LLQYGAEADARDQMQMTPLHKAAMRGSIEVAEALLAHGADVSARHCQGGTPLDIAVQS
                     GHDEFADLLRRHGATHGLE"
     gene            1066..>1784
                     /locus_tag="PLQ54_17290"
     CDS             1066..>1784
                     /locus_tag="PLQ54_17290"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOS95073.1"
                     /translation="MAHDDHDESPHGKADLMRRYLLALPGAGRVAWETYQAFTAHKSA
                     ALAGAVAFYTLLGLSPLLFVVGAVVRLVYGDGYPVLDRALPALQPVIPLQRRELAELL
                     GNFVSASSVPIGSVGFIGLLWTASSAFNLTYEAICHAGGVPARFIRRRLMGLAAPLVL
                     GMVALAMFFAASAAPLARLLFHPPIDLGFLGRLALFGRHLVNGLGVAPVLFVVYKACA
                     GRHLGSRHAVVSALIFGPVWE"
BASE COUNT          261 a          612 c          603 g          308 t
ORIGIN      
        1 gcggctgacg ggcaagcggg tggtcgtgcc atcatccctg ctcactccag cccatgggtg
       61 gcgccgtggc ggcggagaag gtcggcgaac tcatcgtggc cggactgcac cgcgatgtcg
      121 agtggcgtac caccttggca gtgccgggcg ctcacgtcgg cgccgtgggc cagaagggcc
      181 tcggccacct cgatgctgcc acgcatggcc gctttgtgga gcggggtcat ctgcatctgg
      241 tcgcgagcat cggcctcagc accatattga aggaggacat ccagcacctc agcgtgctcg
      301 aagtaagacg cccagtgcac aggagtgaaa ccagtcttgg gctccgtgag gtccggatcg
      361 gctccggccg cgagcagcac ccgggcgacc tccacatgcc ccttgtgagc cgctttcacc
      421 agcggcgact ggtcaaacgc gtccacggcg tcgacctcgg cgccgtgctt caccaagacg
      481 gcggccgtct ctgcgtgccc ctgggtcgct gcccagtgca gcggcgtgcc cccgccgcca
      541 tcgcgcgggt ctacggcggc cccccgttcg aggagcagcc gtgcaacgct ggcgtggccc
      601 gcgaacgccg cattatgtag gggcgtcatg gcaccgtgag ggacccagac ttgcgcctgg
      661 gtcacgttgc cctgatcatc ggcacgcaga atgggcgcag ccaggtcgac cctgggaccc
      721 atgggatcca gagccgcgcc ggcgtcgatg aggagagcca cgacatcgac atggcccatg
      781 cgcgcggccg catgcagggg cgccatgccc tcctggtctc ggcggttggg gtccgcgccc
      841 gccgagagca ggcggcggac gacgcccaca cggcccttga gagccgcggt gtgcaggtcc
      901 agcccagcat tctcgtcggc ttggtgcatc caaccatcct tcgagccaga ggcgtcggtt
      961 ctcgtgacgt gaacgcatta tacattcact ggcgactcac tggcgcagat cggcatgccg
     1021 aacgcagaca gaggggcgcc ccattggccg cccgaagcaa ccagcatggc ccacgacgac
     1081 cacgacgaga gcccacatgg gaaggccgac ctgatgcggc gatacctgct ggccctcccc
     1141 ggcgccggtc gcgtcgcatg ggagacctac caggcgttca ccgcccacaa aagcgccgct
     1201 ctggcgggtg ccgtggcgtt ctacaccctg ctcggactca gcccattgct gttcgtcgtc
     1261 ggcgcggtgg tgcggctggt ctacggagac ggctacccgg tcctcgaccg agcgctgcca
     1321 gccctgcagc cggtgattcc cctgcagagg cgtgagctgg cggagctact gggcaacttc
     1381 gtctcggctt ccagtgtacc catcgggagc gtgggcttca tcggcctgct atggacggcc
     1441 tcttcggcct tcaacctgac ctatgaagcg atctgtcatg cgggcggagt ccccgcccgg
     1501 ttcatacggc gccggctcat ggggctggcg gcccccctgg tcctggggat ggtggccctc
     1561 gccatgttct tcgcggcatc ggcggcgcca ctggcccgtc tgctcttcca cccgcccatc
     1621 gacctgggct ttctgggccg gctggctctg ttcggcaggc atctcgtgaa cggtctgggt
     1681 gtggcgcccg tgttgttcgt ggtctacaag gcctgcgccg gtcgtcatct ggggagccgg
     1741 catgcggtgg tcagcgccct catcttcgga ccggtgtggg agtt
//
LOCUS       DAOSPL010001117         1782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_66529_length_1782_cov_2.707006, whole genome shotgun sequence.
ACCESSION   DAOSPL010001117 DAOSPL010000000
VERSION     DAOSPL010001117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66529_length_1782_cov_2.707006"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PLQ54_17295"
     CDS             <1..630
                     /locus_tag="PLQ54_17295"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95074.1"
                     /translation="LLPGADPVQKVSITSRGHALGFTLQVPEEDRHMYGRQHLLDKIC
                     STLGGRAAEELVLGDQFSGAASDLEQVADIARAMVCQFGMSERLGPLSIGRRDRQIFL
                     GRDLVEERQISEDTARIVDEEIQSIIRHSHDRALSLMEQHRALLDAMVEALLERETLR
                     TEELIDLVREIEGPELADRVARPRTEVTPSGLEGGGPSAGRASGGPPEG"
     gene            745..>1782
                     /locus_tag="PLQ54_17300"
     CDS             745..>1782
                     /locus_tag="PLQ54_17300"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008859413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOS95075.1"
                     /translation="MALSNIEIAQKAQLKDIREIAEVAGIRDDELECYGRNKAKVDLG
                     VLRRLADKPDGRLVLVTAVTPTLTGEGKSTVTVGLGQALHAMGKRAMICLREPSLGPC
                     FGMKGGACGGGYSQVVPMEDINLHFTGDIHAVTAAHNLLACLLDNHLHWGNDLGVDIR
                     RITWTRVQDVCDRSLRDVLVGLGGANGGIPRETSFRITAASEVMAILCLAGGIEDLKS
                     RLAKIIVGYTSDRAPVTAADLKATGGMALLLKDALKPNLVQTLEGSPAFVHGGPFANI
                     AHGCNSVLATRTALKLADYVITEAGFGSDLGAEKFFNIKCRAAGLRPSAAVLACTLKA
                     VKRSGGGDEKNP"
BASE COUNT          309 a          538 c          638 g          297 t
ORIGIN      
        1 ctcctgcccg gtgccgaccc ggtgcagaag gtctcgatca cctcgagggg ccacgccctg
       61 ggcttcaccc tacaagtccc ggaagaggac cggcatatgt acggccgaca gcatctcctg
      121 gacaagatct gctcgaccct ggggggcagg gccgccgagg aactggtcct aggcgatcag
      181 ttcagcgggg cggccagcga cttggagcag gtggcggaca tcgcccgggc catggtgtgc
      241 cagttcggca tgagcgagcg gctgggcccg ctgtccatcg gccgccggga ccgccagatc
      301 tttctgggca gggacttggt ggaagagcgg cagatcagcg aggacacggc tcgcatcgtc
      361 gatgaggaga tccagtcgat tatccgacac tcccacgatc gtgcgctgag cctgatggag
      421 caacaccggg cgcttctgga tgccatggtg gaggcgttgc tggagcggga gacgcttcgg
      481 accgaggagc tgatcgatct ggtgcgcgag atcgaggggc cggagctggc cgaccgcgtc
      541 gcgcgccctc ggaccgaggt gaccccgagc ggactggagg gtgggggacc gtcggcgggc
      601 cgagcgagtg ggggccctcc ggaggggtag ttggcttgcc acagcggaag gcgaagcgcg
      661 ttcgccttcc gctgcgctgt gccggcccgc gtgtggcggg ccatctggat cgcaccgagc
      721 acggtgctgt ggagcgtgta gcgattggcc ctgtcgaaca ttgagatcgc tcagaaggcg
      781 cagctcaaag acatccgtga gatcgccgaa gtggcaggca tccgcgatga cgaactggag
      841 tgctacggcc ggaacaaagc caaggtggac ctcggtgttc tccgccggct cgctgacaag
      901 ccagacggtc gcctcgtgct ggtcaccgcc gtgacaccga cgttgacggg cgagggcaag
      961 tcgacggtga ccgtggggct cgggcaggcc ctccacgcga tgggaaagcg ggccatgatc
     1021 tgcctgcgcg agccatcgct gggtccctgt ttcggcatga agggaggggc ctgtggcggt
     1081 gggtactccc aggtggtgcc catggaggac atcaacctgc acttcaccgg ggacatccat
     1141 gcggtcacgg cggcccacaa tctgctggca tgcctcctgg acaaccacct ccattggggg
     1201 aacgatcttg gcgtagacat tcgccggatc acgtggacgc gcgttcagga cgtgtgcgac
     1261 cgtagcctgc gcgacgtctt ggtcggtctg ggtggagcaa atggcggaat cccgcgagag
     1321 accagcttcc gcatcacggc ggcttcggag gtcatggcca ttctctgtct cgcgggcggg
     1381 atcgaagacc tgaagtcgcg tctggcaaag atcatcgtcg ggtatacctc agaccgagcg
     1441 ccggtcactg cggcggatct gaaggcgacc ggcggcatgg cgctgctact caaggacgct
     1501 ctcaagccca acttggtcca gacgcttgaa ggctccccgg cgttcgtgca tggtgggccc
     1561 tttgcgaaca tcgcccacgg gtgcaactcg gtgctcgcca cgcgaaccgc gctgaagctc
     1621 gcggactacg tcattacgga ggcggggttc ggttcggatc tgggggccga gaagttcttc
     1681 aatatcaaat gccgggctgc aggcctgcgg ccctctgccg ctgtcctggc gtgcacgctc
     1741 aaggcggtca agcgctccgg gggcggagac gagaagaacc cc
//
LOCUS       DAOSPL010001118         1780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_66614_length_1780_cov_3.850435, whole genome shotgun sequence.
ACCESSION   DAOSPL010001118 DAOSPL010000000
VERSION     DAOSPL010001118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66614_length_1780_cov_3.850435"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..1485)
                     /gene="eno"
                     /locus_tag="PLQ54_17305"
     CDS             complement(187..1485)
                     /gene="eno"
                     /locus_tag="PLQ54_17305"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOS95076.1"
                     /translation="MTAITDVNARQILDSRGNPTVEVEVWLESGEFGRGAVPSGASTG
                     AHEAVELRDGNSAVYSGKGVLNAVENVNTVIGPAILDMDALEQTAIDELMIELDGTPN
                     KGKLGANAILGVSIAVAHAAAKTVGLPLYRYLGGVSARELPVPMMNILNGGKHAEGSV
                     DLQEFMAMPVGATSFAQALQMGAETFHALKSVLKKHGYSTQVGDEGGYAPSLKANEEA
                     IQVILEAIEMAGYKPGDEIAIALDPAATELYEDGKYVCRGEGVTRTSAEMVDFLASLV
                     DKYPIVSIEDGLAEDDWDAWVELTKRVGHRVQLVGDDLYVTNTERLARGIESRASNSI
                     LIKVNQIGTLTETIAAVEMAKRAGFTAVVSHRSGETEDTTIADLVVGLNAGQIKTGAP
                     CRSERVAKYNQLLRIEEELGEAARYLGRETFYNVRANRTS"
     gene            complement(1569..>1780)
                     /locus_tag="PLQ54_17310"
     CDS             complement(1569..>1780)
                     /locus_tag="PLQ54_17310"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95077.1"
                     /translation="VVERIVRQFVAAARQVDPKFEIFICGEHGGDLYTIGRLHDMGFT
                     GTSMSPGGIYRSILQAAHEQIKNPR"
BASE COUNT          289 a          631 c          530 g          330 t
ORIGIN      
        1 gcgcatccag ccgacggacc tccaggtact cgggatacca gtcgacaacc atccacgcca
       61 gcagcgctgc cacgccgcag agcgccaccc cccgagacat ggaaccgggc tggaggccgc
      121 ggttagcagc ctccgggccc ggtcgtccat agccactcgt gtcctgcacg cctacggcga
      181 atcccctcag ctcgtccggt tggcccgcac gttgtagaag gtctccctgc ccaggtaccg
      241 cgcggcctcg cccagttcct cctcgatccg gagcaactgg ttgtacttgg ccacgcgctc
      301 ggaccggcac ggcgcgccgg tcttgatctg cccggcgttg agaccgacca cgaggtcggc
      361 gatggtggtg tcctcggtct cacccgaccg atggctcacc accgcagtga agccagccct
      421 cttggccatc tcgacggccg cgatggtctc ggtgagtgtt ccaatctggt tcaccttgat
      481 caggatcgag ttcgacgcgc gagactcgat cccccgcgcg agccgctctg tgttcgtgac
      541 ataaaggtca tcgcccacca actggacccg atgacccacc cgcttggtca actcgaccca
      601 ggcgtcccag tcgtcctccg ccaggccgtc ttcgatggac acaatcgggt acttgtccac
      661 cagactggcg aggaaatcca ccatctcggc cgacgtccgg gtgacgcctt cgccccgaca
      721 cacgtacttc ccgtcctcgt acagctcggt ggcggcggga tccagcgcga tggcaatctc
      781 gtccccaggc ttgtacccgg ccatctcaat ggcctccagt atgacctgga tcgcctcctc
      841 gttggccttc aggctcggcg catagccacc ttcgtcgccg acctgtgtcg agtacccatg
      901 cttcttcagc acgctcttga gtgcgtggaa tgtctcagca cccatctgca gtgcctgggc
      961 gaacgaggtt gcacccacgg gcatcgccat gaactcctgc aggtcgacgc tgccctcggc
     1021 atgcttcccc ccgttgagga tgttcatcat cgggacaggc agttcgcgcg cagaaacccc
     1081 acccaggtag cggtagagag gcagcccgac cgtcttggct gccgcatggg ccaccgcaat
     1141 ggagacgccc agaatggcgt tggccccgag cttgcccttg ttgggcgtcc cgtccagctc
     1201 gatcatcagc tcgtcgatgg ccgtctgctc gagcgcatcc atgtccagaa tggccggtcc
     1261 aatgacggtg ttcacgttct cgacggcgtt gaggacgccc ttgcccgagt agacggcgga
     1321 gttcccgtcc cggagctcga cggcctcatg agccccagtg gaggcccccg aggggacggc
     1381 gccgcgaccg aactccccac tttcgagcca gacctcgacc tcgaccgtag ggttgcctcg
     1441 ggagtccagg atctggcgtg cgttgacgtc ggtgatggcg gtcatgtggc ttctccccta
     1501 ttcgctaggg gccgcctcag ctagacggcg gcccctccag tatcgcgatg cggtcaccgc
     1561 ttgcagccct agcgagggtt cttgatctgc tcgtgggcgg cctgcaggat ggaacggtag
     1621 atcccgcccg gcgacatgct ggttccagtg aagcccatgt catgcagccg gccgatcgtg
     1681 tacaggtcgc cgccgtgctc gccgcagatg aagatctcga acttcgggtc aacctgtcgc
     1741 gccgccgcca cgaactggcg cacgatgcgc tccacgacgg
//
LOCUS       DAOSPL010001119         1779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_66700_length_1779_cov_2.542343, whole genome shotgun sequence.
ACCESSION   DAOSPL010001119 DAOSPL010000000
VERSION     DAOSPL010001119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66700_length_1779_cov_2.542343"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PLQ54_17315"
     CDS             complement(<1..969)
                     /locus_tag="PLQ54_17315"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS95078.1"
                     /translation="MTGLAVERQRRRNFALHVWEGAIYFAGVEFASATSIIPVLLERL
                     GASRAIIGLAASFTYVGTAAQILGVPAMEAIPRRKRVLVRLGLFMRLPYAVMAVAVVL
                     LATRAPRACIATVLWLTLVTSLAGGLTTPAWLDLIRSTIPRARLSSLFGLRHALGGAL
                     IAGTGATATLVLTTLAFPASFAVLLGLACAVTMISWALFANVLDSPPHRPSRTRPGFR
                     AHMLQLVADVRGDVVFLRLLLARCLYLVGRAGEAFYTVCALERFHSRDAAAGTFMLTM
                     ALARCASALLFSKLTDHIGRRATAALSSVLGAAAGILAIVAPNESAF"
     gene            complement(966..1763)
                     /locus_tag="PLQ54_17320"
     CDS             complement(966..1763)
                     /locus_tag="PLQ54_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95079.1"
                     /translation="MAVPQIEFAGLVVSRLMVGGNPFSGNSHQPGSASREMRDYFTTA
                     RIIEVLARCESLGITTFVGRADAHIIRVLHEYWNAGGRVAWIAQTAPEMRSVDENIRR
                     AVGEGARAVYIHGGWFDERWEAGDLADVDRALATIHSLSVPAGIGGHIPEYHLAAVGQ
                     LAADFHMVSCYRCGSIHAAKGETFDPADFAPALAAMGQLPRPCIAFKLLGAGRFDPAV
                     QIPAALRAIKPTDAILVGFYPKHQPRQVEDVVALVQRGVAVPGVEPS"
BASE COUNT          335 a          594 c          615 g          235 t
ORIGIN      
        1 gaaggcgctc tcgttgggcg ccacgatggc caggatgccc gctgccgcgc ccagcaccga
       61 cgagagcgcg gcggtcgcgc gtcgtccgat gtgatcggtc agcttcgaga acaacagagc
      121 actcgcgcag cgggccaggg ccatggtcag catgaaggtg cccgccgcgg cgtcccgact
      181 gtggaaccgc tcgagggcac agacggtgta gaaagcctcg cccgcccgtc ccaccaggta
      241 gaggcaccga gcgagaagca gtctgaggaa aacgacatcg ccacgcacgt cagccacgag
      301 ctgaagcata tgagccctga atccgggacg agtgcggctg ggccggtgag gtggcgagtc
      361 gagaacgttt gcgaacaggg cccaggagat catagtcacc gcgcaggcca agcccaagag
      421 gaccgcaaag ctcgcgggaa acgccagcgt ggtgagaacc agcgtcgcag tggcccccgt
      481 gccggcgatg agcgcgcccc ccaatgcgtg ccggagaccg aacagcgatg acagccgggc
      541 tcgggggatg gtgctgcgga taaggtccag ccaggccggg gtggtcaggc ccccggcgag
      601 gctggtgact agagtcagcc atagcaccgt ggcgatgcag gccctcgggg ctcgggtggc
      661 gagcagcact acggccacag ccatcacggc gtagggcaac cgcatgaaga gacccaggcg
      721 gaccagcacg cgcttgcgac ggggtatcgc ctccatcgca ggcacgccca ggatctgagc
      781 agccgtgcca acataggtga agctcgcggc gaggccgatg atcgcgcggg aggcaccgag
      841 gcgctcgagg agcaccggaa tgatcgatgt ggcactcgcg aactcgacgc cggcgaagta
      901 gatggcgccc tcccacacat ggagggcgaa gttgcgccgc cgctgtctct cgacggcaag
      961 cccggtcacg acggctcgac gcccgggacc gcgactccgc gctgcaccag tgccaccacg
     1021 tcctccacct gccgcggctg gtgcttgggg tagaagccga cgaggatggc gtcggtgggt
     1081 ttgatcgccc gcagcgcggc tgggatctgc acggccgggt cgaagcgccc ggcgcccaga
     1141 agcttgaagg cgatgcaggg ccgcggcagc tggcccatgg ccgccagagc gggggcgaag
     1201 tcggcgggat cgaatgtctc acccttcgca gcatggatgc tgccgcagcg gtagcagctg
     1261 accatgtgga agtcggcagc aagctggccc acggcggcga ggtggtactc ggggatgtgt
     1321 cccccgatac cggccgggac gctcaggctg tgaatggtcg ccagcgcccg gtcgacatca
     1381 gccaggtcac ccgcttccca gcgctcgtcg aaccagcccc catgaatgta gacggctcgg
     1441 gccccctcgc caacggcccg ccggatgttc tcatcgaccg agcgcatctc cggcgccgtc
     1501 tgcgcaatcc aggcgacccg gccgcccgca ttccagtact catgcaacac tcggatgatg
     1561 tgggcatcgg cacggcccac gaaggtcgtg atgcccaatg attcacaacg agcgaggacc
     1621 tcaatgatcc gcgccgtcgt gaagtagtcg cgcatctccc ggcttgcact gcccggctga
     1681 tgtgagttcc cgctgaaggg gttcccgccc accatcaggc gcgagacgac gaggcccgcg
     1741 aactcgatct gcgggaccgc catcgctcag gccccctcc
//
LOCUS       DAOSPL010001120         1779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_66703_length_1779_cov_2.225638, whole genome shotgun sequence.
ACCESSION   DAOSPL010001120 DAOSPL010000000
VERSION     DAOSPL010001120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66703_length_1779_cov_2.225638"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..652
                     /gene="ndhC"
                     /locus_tag="PLQ54_17325"
     CDS             296..652
                     /gene="ndhC"
                     /locus_tag="PLQ54_17325"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOS95080.1"
                     /translation="MLESYFPILVMGVFAAAVAGGMCLLARLVGPRHATAQKAEPYEC
                     GMWPVGDARRRFAVKFHVVAMLFILFDVELAFFYPWAVLFRDLRLFGLIEMGIFTAIL
                     VAAYAYVWRAGGFEWD"
     gene            643..1095
                     /locus_tag="PLQ54_17330"
                     /pseudo
     CDS             643..1095
                     /locus_tag="PLQ54_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            1116..1643
                     /locus_tag="PLQ54_17335"
     CDS             1116..1643
                     /locus_tag="PLQ54_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOS95081.1"
                     /translation="MSEATPPEVTPRSGGTTLVRAAVAAIEAATGRGPLEISDFRGTP
                     CLRVDAHSVVAACQSLRDASALDFALLVDLTAVDWSGCRPGCEPRFDVIYQLMSLTRN
                     ERLFLAVGVPSDDPTVPSVIGLWHAAGFLEREVFDLMGIRFDGHPDLRRLVLPEDWQG
                     HPLRKEYPLRGDDRP"
     gene            1640..>1779
                     /locus_tag="PLQ54_17340"
     CDS             1640..>1779
                     /locus_tag="PLQ54_17340"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904539.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOS95082.1"
                     /translation="MTGAPVEAGRETLTLNLGPQHPSTHGVLRLVLTLEGETVVDCMP
                     DVG"
BASE COUNT          245 a          589 c          601 g          344 t
ORIGIN      
        1 ggtattgcct cgcctcctgg catttcgggc actctgcccc cgtcgagtaa cttagtgata
       61 ccatcgtgat ttccatcggc gggccgcctc tccggggaca gtctgccacc cgtgggaacc
      121 ttcgggacgc cgccaaacgc tcctgcccgc atcggaaggt ggggccggca tcggggcgaa
      181 atgcttcggt tggcggggcc gagcgctggg ccgtccgcag gaccccggct cgcgcccgcc
      241 ggagtgggtc gcgcaacgag acgccgtttg aacctgttga tggagctcgt gggcgatgtt
      301 ggagagctac ttccccatcc tcgtcatggg cgtgttcgcc gccgcggttg ccgggggcat
      361 gtgcctgctc gcgcgcctgg tgggtccccg gcatgctacc gcacagaagg cggagccata
      421 cgaatgcggc atgtggcctg tgggggatgc ccggcggcgc ttcgcggtga agtttcacgt
      481 cgtggcgatg ttgttcatcc ttttcgatgt ggagctcgcg ttcttctacc catgggccgt
      541 gctctttcgt gacctgcgcc tgttcggtct gatcgagatg gggatcttca ccgctatcct
      601 ggtggccgcg tacgcgtacg tgtggcgggc ggggggcttc gagtgggact agaggagagg
      661 atcccgcccg gcatcttctt gaccgccgtc gagcggatcg tgaactgggc gcgcgaatac
      721 tcgacctggc ccctgtcgat gggcttggcc tgttgcgcca ttgagatgat ggccacggcg
      781 gccgcccgga ccgacctgtc gcgctttggc atggaggtgt tccgcccctc gccccgccag
      841 gcggacctga tgattgtggc gggacgagtc agcgtgaaga tggccccggt catccggcac
      901 ctctacgatc agatgcccga gcccaagtgg gtcatcgcca tgggcgcctg cgcctcctcc
      961 ggcggcgtgt tcgacaacta cgccatcgtt cagggcgtcg accatgtggt ccccgtcgat
     1021 gtctatgtcc ctgcctgccc gccgcggccc gagatgctca tacacgggat cctccaactg
     1081 cacgagcaga tccgtcagag ccggttggtg cagcgatgag cgaagcgaca cctccggagg
     1141 tgacaccgcg gtccggtggt accactctcg tcagggcggc cgttgcggcc atcgaggccg
     1201 ccacgggacg gggacccctt gagatcagcg acttccgagg cactccatgc ctccgcgtcg
     1261 atgcacactc cgtcgtcgcg gcctgccaga gcctccgtga tgcatcggcg ctcgacttcg
     1321 ccctgttggt cgatctgacc gccgtcgact ggtcgggttg tcgccctggc tgcgagccgc
     1381 gctttgacgt catctaccag ctcatgtcgc tgacgcgcaa cgaacggctg ttcctggctg
     1441 tcggagtgcc gtcggatgac ccgacggtgc ccagcgtcat tgggctctgg cacgccgccg
     1501 gcttcctcga gcgcgaggtc ttcgacctca tgggcatccg cttcgacggt caccctgact
     1561 tgcggcggct ggtgctgccc gaggactggc aggggcatcc gctacgcaag gagtacccgt
     1621 tgcgggggga cgaccgccca tgaccggtgc ccccgttgag gcaggccgcg agactctgac
     1681 cctgaacctg gggcctcagc acccgagcac gcacggggtg ctccggctcg tcctcacgct
     1741 cgaaggcgag accgtcgtcg actgcatgcc cgatgtggg
//
LOCUS       DAOSPL010001121         1778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_66725_length_1778_cov_3.046431, whole genome shotgun sequence.
ACCESSION   DAOSPL010001121 DAOSPL010000000
VERSION     DAOSPL010001121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66725_length_1778_cov_3.046431"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="PLQ54_17345"
     CDS             complement(<1..1075)
                     /locus_tag="PLQ54_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOS95083.1"
                     /translation="MAGGKTLVVIPVLLVAASVSSALAADLCAEGLALEDVGSIVNYY
                     IPQRLTLQAEKPEALQAEPTYEGTPRYAALKMGDGADSIITVAVDEKLAEDGTGTRRI
                     YIDANNDNDLTNDGDGAWRRESAQSLMTEVTVKTFYAGDGGGAAVDYRIALSRLKDQL
                     PDQILYYRRSARVGELKIGDQQYKVALVDDNTNGLYNDLAPAQGQRGPGVTLVIDRDQ
                     DGTFVPDFGGPEVFAGHEPFCIAGTTYELDQVSPAGESFTIRVSDEVVAERAYIIVGR
                     PAPEIDASDIDGKPIKLSDYRGRVVLIDFWATWCGPCRQEMPKVIAAYNELHEKGFDV
                     LGISLDEGDEGVAKLRQYIGENGM"
     gene            complement(1129..>1778)
                     /locus_tag="PLQ54_17350"
     CDS             complement(1129..>1778)
                     /locus_tag="PLQ54_17350"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOS95084.1"
                     /translation="YDSPDNFAAGPSSFEAIWSRYANLVAASPNPNVGLAVTEWNAQS
                     TDWRTGLFCGGLLNVMERQRVVRMASPALLLRRVDATDWDNAFINHDHRGWFPAPNYV
                     VMRLYREHFQPNRVALEVTGTLDAVATVDDAGNRLVLKVVNADAKAVDATVRLGGGFS
                     PRSGTAWTVRAGLRERNTLDRPNRIGARAERLAVARGEFGHRFPAYSVTVLELDR"
BASE COUNT          287 a          619 c          552 g          320 t
ORIGIN      
        1 gcatgccatt ctcgccgata tactgacgca gcttcgccac accctcgtcg ccctcgtcga
       61 ggctgatgcc gagcacgtcg aagcccttct cgtgcagctc gttgtaggcg gcgatgacct
      121 tgggcatctc ctgcctgcac ggcccacacc acgtggccca gaagtcgatc aggacgaccc
      181 ttcccctgta gtcggagagc ttgatgggct tgccgtcgat atcgctcgca tcgatctcgg
      241 gtgcgggacg tcccacaatg atgtaggcgc gctcggcgac aacctcatcc gagaccctga
      301 tcgtgaacga ctcgcccgcc gggctgacct ggtcgagctc atacgtcgtg cccgcgatgc
      361 agaagggctc gtggccggcg aacacctccg gtccaccgaa gtcgggcacg aaggtaccgt
      421 cctgatcgcg atcgatcacg agagttaccc cggggcctcg ttgcccctgg gccggcgcca
      481 ggtcgttgta gagcccgttg gtgttgtcgt caaccagggc caccttgtac tgctggtcac
      541 cgatcttcag ctcgcccacc cgggccgacc tccggtagta gaggatttgg tcgggtagct
      601 gatccttgag ccgggagagg gcgatccggt agtccacagc cgcccctccg ccgtcgccgg
      661 cgtagaatgt cttcacggtg acttcggtca tcaggctctg ggcgctctcg cgccgccacg
      721 cgccgtcgcc gtcgttggtc aggtcgttat cgttgttcgc gtcgatgtag atcctgcggg
      781 tgccggtgcc atcctcggcc agcttctcat cgacggcgac cgtgatgatc gagtcggcac
      841 catcgcccat cttcagcgcc gcgtatcggg gcgtcccctc gtaggttggc tccgcctgga
      901 gcgcctcggg cttctccgcc tgcaaggtga gacgctgggg gatgtagtag ttgacgatgg
      961 agcccacgtc ctcgagcgca aggccctcag cgcatagatc agccgcgagc gccgacgaga
     1021 ccgatgcggc caccagtagt accggaatga cgaccagcgt cttgccccct gccatggagc
     1081 cgaccgcctc tcaggatgtc gcccactaca acgcgctgag gcgaagcctc agcggtccaa
     1141 ctcgagtacc gtgaccgagt aggccgggaa ccggtggccg aactcgccgc gggccaccgc
     1201 caggcgctcc gcccgagcac cgatgcgatt cggccggtcc agcgtgttgc gttcgcgaag
     1261 cccggccctc acggtccacg cggtgccgct ccgaggcgaa aagccgccgc cgagcctcac
     1321 ggttgcgtcg accgctttgg cgtcagcgtt cacaaccttc aacaccaagc ggtttccggc
     1381 gtcatccacc gtagccacgg cgtcgagggt ccccgtgacc tcgagcgcca cccgattcgg
     1441 ctgaaagtgc tcccgataaa gcctcatcac cacgtagttg ggtgccggga accagccgcg
     1501 gtggtcgtgg ttgatgaacg cgttgtccca gtcggtggcg tccacgcggc gcaacagcaa
     1561 cgccgggctg gccatccgca cgaccctctg ccgctccatg acgttcagca agccgccgca
     1621 gaacagcccc gttcgccagt ccgtgctctg ggcgttccac tcggtcacgg cgagccccac
     1681 gttcgggttc gggctggccg cgaccagatt ggcatagcgc gaccatatgg cctcgaagct
     1741 cgacggaccg gccgcgaagt tgtccgggct atcataca
//
LOCUS       DAOSPL010001122         1777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_66768_length_1777_cov_3.328688, whole genome shotgun sequence.
ACCESSION   DAOSPL010001122 DAOSPL010000000
VERSION     DAOSPL010001122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66768_length_1777_cov_3.328688"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PLQ54_17355"
     CDS             complement(<1..419)
                     /locus_tag="PLQ54_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95085.1"
                     /translation="MPTNVRLGLVPSFRFTWTPWTQQMRDETLAVMAATRGVEVVVPS
                     PSTDGRADAGAGITPYGAVQTLDQAEAVAGYFAQQKIDGLVICPTDFGDERSACKVAE
                     LLQVPTLLLATKEPPAPPSASLERVSDSYCGTLSIAAG"
     gene            complement(430..1476)
                     /locus_tag="PLQ54_17360"
     CDS             complement(430..1476)
                     /locus_tag="PLQ54_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOS95086.1"
                     /translation="MVGPREVIAVHKELIAVAILSSVVAWGADEPWVTFDGFQGPGEG
                     RHVVLITGDEEYRSEESMPQLARILAARHGFRCTVLFAVTPETGEIDPTVVDHIPGLE
                     ALDSADLMVLFTRFRELPDEQMRHIIDYTNSGRPIIALRTATHPFNYTKHLDSPYACW
                     SFRATGDFDGGYGRQVLGETWVNHYGVHQKESTRGLVAPGMEGHPIVRGCEDIWGPSD
                     VYQITTLTGDSEPVIMGQVLTGMDPADPPNPDRQLVPVAWTKTYTGTQGKPSRVFTTT
                     MGHAGDLVSEGFRRLLVNAVYWAVGLEEQIPTRADVALVGRYEPTPIGLGGHKRGVRP
                     RDHRLVPTDFANGM"
     gene            1586..>1777
                     /locus_tag="PLQ54_17365"
     CDS             1586..>1777
                     /locus_tag="PLQ54_17365"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOS95087.1"
                     /translation="MVLPMLTNVVSVIGLAILVVCITQRLRAPSIVGYLLTGVLVGSG
                     GLGIVQSHKDVEFLAEMGVV"
BASE COUNT          272 a          582 c          598 g          325 t
ORIGIN      
        1 cccgccgcga tggacagcgt gccgcagtac gagtctgaga ctctctcgag tgaggcgctc
       61 ggaggcgccg gcggctcctt ggtggccagc aggagtgtcg gcacctgcag gagctcggcc
      121 accttgcacg ccgaccgctc atcgccaaag tcggtcgggc agatgaccag gccgtcgatc
      181 ttctgttggg cgaaataccc ggctacggcc tcggcctgat cgagcgtctg gacggcgccg
      241 tagggtgtga tcccggctcc cgcgtctgct cggccatcgg tcgatggcga tggcaccacg
      301 acctcaacgc cacgcgtggc cgccatgacc gccagggtct catccctcat ctgctgggtc
      361 cacggcgtcc acgtaaagcg gaacgatggc acgaggccca gtctcacgtt cgtcggcatt
      421 gcgcttgcct cacattccgt tcgcgaagtc agtggggacg agccggtggt cgcgcggccg
      481 cacgccgcgc ttgtggccac ccaaaccgat gggcgtcggc tcgtagcggc cgaccagcgc
      541 cacatccgcg cgagtcggga tctgctcctc gagcccgacc gcccagtaga cggcgttcac
      601 caggagccgg cggaacccct cgctgaccag gtcgccggca tgccccatgg tcgtggtgaa
      661 cacacggctc ggcttcccct gggtaccggt gtaggtcttc gtccacgcca cgggcaccag
      721 ctgtctgtcc gggttgggtg gatcggccgg gtccataccc gtgagcacct ggcccatgat
      781 gaccggctcg ctgtcgccag tgagtgtggt gatctggtag acgtccgaag gcccccagat
      841 gtcctcacag ccccgcacga taggatggcc ctccatcccg ggcgctacga ggccgcgcgt
      901 gctctccttc tggtggacgc cgtagtggtt gacccaggtc tcgccgagca cctgacggcc
      961 gtagccgccg tcgaagtcgc ccgtggctcg gaagctccag caggcgtaag ggctgtcgag
     1021 gtgcttggtg tagttgaatg ggtgtgttgc ggtgcgtagc gcgatgatgg gccggcccga
     1081 gttcgtgtag tcgatgatgt gcctcatctg ctcgtccgga agctcgcgga atcgcgtgaa
     1141 cagcaccatc aggtcggcgg agtccagtgc ctccagcccc gggatgtggt cgacgaccgt
     1201 ggggtcgatc tcacccgtct ccggggtcac ggcgaacagc acggtgcatc ggaaaccgtg
     1261 ccgagcggcc aggattcgag cgagctgggg catcgactcc tccgaacggt actcctcgtc
     1321 gccggtgatg agaacgacat gcctgccctc tccgggcccc tggaagccgt cgaaagtcac
     1381 ccagggctcg tcggcacccc aggcgacgac tgaactcagg atcgccacgg cgatcagctc
     1441 cttgtgcacc gcgatcacct cacgcggccc caccatgcag catgaccttg cggggaccgc
     1501 ctgttccgga gttacgttgc ggcgacctgc ccgtgctggc gaggataacg ggcgcaggtt
     1561 ccggaatcgc tggcgggggt gggtcatggt tctccccatg ctcacgaacg tcgtgtcggt
     1621 gatcggtctg gccattctgg tcgtctgcat cacacaacgg ctgcgcgcgc cgtcgatcgt
     1681 cgggtatctg cttactggag ttctggtcgg ctcaggcggg ttggggatag ttcagtcgca
     1741 caaggacgtc gagttcctgg ccgagatggg cgtggtt
//
LOCUS       DAOSPL010001123         1777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_66777_length_1777_cov_2.679443, whole genome shotgun sequence.
ACCESSION   DAOSPL010001123 DAOSPL010000000
VERSION     DAOSPL010001123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66777_length_1777_cov_2.679443"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..305)
                     /locus_tag="PLQ54_17370"
     CDS             complement(108..305)
                     /locus_tag="PLQ54_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOS95088.1"
                     /translation="MSQPWMSAEEIAAHLGVTKDTVYSWIADRGMPAHKIGRLWKFQA
                     DEVDEWVRRGGASTPTRGDSA"
     gene            586..813
                     /locus_tag="PLQ54_17375"
     CDS             586..813
                     /locus_tag="PLQ54_17375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HOS95089.1"
                     /translation="MGTEETAQVRLRRAATGRRRDDRDLGVDDVVRLEIRLPAAVAAA
                     LFDQAHSTHEPVSRVAARLLRSSLDVPRDRP"
     gene            956..>1777
                     /locus_tag="PLQ54_17380"
     CDS             956..>1777
                     /locus_tag="PLQ54_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOS95090.1"
                     /translation="MPSSRPAHRRVNEQRRQPALTAAQAIEQYRPHLATREEWAVVAD
                     FVRAAVRDLEPDGPHRAREWLRTVTALAAWSQREGIPLDREEVFAPDNIRRYAEIGCP
                     ELSTPSTATRRAVLRTMGRALTEEAPWPAPEPMLPQRLRHPPYSARDEQRLRSLRLPT
                     RQQQRRLDAVLALGLGAGLWAREYLTATPSDLRTINGLTHLQVQGTRARKVVVRDRDA
                     QTLHRLAAEHPLENFAGFRASEWDRSRTWNAIRAIDTPTDLKLETERLRSTWLVHH"
BASE COUNT          293 a          648 c          565 g          271 t
ORIGIN      
        1 gaacgcgaag atcgagaacg tcgaggcggc aatgcgcacc ttcgaccccg gggtgatctc
       61 ctgcttgagg tcgtccccca gtacctcgtt gacgttgtcg atgagcttca cgcgctgtcg
      121 cccctggtcg gcgtactggc cccaccgcgc cgcacccact cgtcgacttc atcggcctgg
      181 aacttccaga gccggccgat cttgtgggcg ggcattcctc ggtcggcgat ccaggagtac
      241 acggtgtcct tggtgacgcc gaggtgggca gcgatctcct cagcgctcat ccatggctgc
      301 gacatcccga cctcctgtgt ccgttcttcc agagactaac ccggtgtgac cgggtgtgtc
      361 tggctgtagt ggcccgccca gccattgggc gccaacctgc tgcccaaatc gacgagccgc
      421 gacgcaccgt gccgagcggg gttctggaac gtgccccggc gcgtcgctac catagaggtc
      481 accaacaccc cgcaggccga ccacctgccg agtgcgtcgg gagacgcgca ggagaagggc
      541 cgccggaggc ggcaacaact tcaaaaggtc ggcaggagaa gggtgatggg aaccgaggag
      601 acagcgcagg tgcggctgcg ccgtgccgct acgggacgtc gtcgggatga ccgcgatctc
      661 ggcgtcgatg acgtggtgcg cctcgagatc cgcctcccag ctgcggtcgc ggctgccctg
      721 ttcgaccagg cccattcgac ccacgagccg gtcagccgcg tagcggcccg gctcctgcgc
      781 agctctctcg atgtcccccg ggaccgccca tgacgtaggc gatccaggtc ggcgcccgac
      841 gacgcgccca gatacggcaa aggccgggat tcgcactccc ggccagccgc tcggacatcg
      901 agttcgcacc tcgtggtccg cttcgaaact tcccgtctca ctgaggagct tctgcatgcc
      961 atcgtctcgc cctgcccacc gccgtgtcaa cgagcaacgc cgtcagccgg cgctcacggc
     1021 cgcccaggcc atagagcagt accgccccca cctggccacc cgcgaggagt gggcggtcgt
     1081 cgccgacttc gtccgcgccg ctgtccgtga cctcgagccg gatgggccac accgagctcg
     1141 cgagtggctt cggacggtca ccgctcttgc cgcgtggtca cagcgggaag ggattccgtt
     1201 ggaccgagaa gaggtgttcg cgccggacaa catccgccgc tacgcagaga tcggttgccc
     1261 ggagctgtcc accccgtcga cagcgaccag gcgggcagtc cttcggacca tgggtcgcgc
     1321 cctcaccgag gaggcaccgt ggcccgcacc cgaaccgatg ctgccgcaac gcctgcgcca
     1381 tccgccctac agcgcccggg acgagcaacg actacggtct cttcggctgc ccacgcgaca
     1441 gcagcagcgt cggctcgacg cagtgcttgc actcggcctc ggcgccggtc tgtgggcacg
     1501 ggagtacctc acggccacac catccgatct gcgcaccatc aacggcctga ctcacctgca
     1561 ggtccagggg acccgggcgc ggaaggtcgt ggtgcgcgac cgcgacgctc agaccctgca
     1621 tcgcttggct gccgagcacc cactggagaa cttcgccggc ttccgcgcaa gcgagtggga
     1681 ccgctcacgg acgtggaacg ccatccgcgc catcgacacc ccgaccgacc tgaagctcga
     1741 gaccgagcgc ctacgcagca cttggctcgt gcaccac
//
LOCUS       DAOSPL010001124         1776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_66818_length_1776_cov_3.555491, whole genome shotgun sequence.
ACCESSION   DAOSPL010001124 DAOSPL010000000
VERSION     DAOSPL010001124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66818_length_1776_cov_3.555491"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..988)
                     /locus_tag="PLQ54_17385"
     CDS             complement(11..988)
                     /locus_tag="PLQ54_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOS95091.1"
                     /translation="MPQPPGDPLLRMYRQMVRIRQFEETVSRLFLEGRLPGTLHLYAG
                     QEACAVGVCEALKPTDWITSTHRAHGHAIAKGVSMDSMMAELFARTTGCAGGYGGSMH
                     MGDPAVGALTAIAIVAGNAPVAAGMALGFRMQATGQVVACFTGDGALNEGAFHEAANM
                     AAIWDLPVIFVCENNLYGASTPIAEVVKLERLSDRAAGYGFPGITVDGMDVQAVYEAM
                     VPVVERARTGGGPTLVECLTYRYTGHSRNDTRGYRTREEEAEWKARDPIPRLAAHLTA
                     SGIATEAEVAAIAEEVAAEIERAVRFAEESPSPAPEDCLRHVFAEEVRG"
     gene            complement(1144..>1776)
                     /locus_tag="PLQ54_17390"
     CDS             complement(1144..>1776)
                     /locus_tag="PLQ54_17390"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOS95092.1"
                     /translation="SSFRNFAELCITRQIITAIKTATRNKHTPLNQYVSFSATPASAM
                     DGEPTVRLLARARQLGATTSMDTAFNPNVDAAALIEPCLPHLDVFIPSIEEAQAITGE
                     SDPGRILDTLDGHGTRTLGVKLGAEGCLIRSSGETRRHAAFEVGVVDGSGAGDAFFAG
                     YLYGVLQGWGADESACFANATAAHCIQAIGCSAGVPTAEEVRAFMRAVAR"
BASE COUNT          260 a          636 c          603 g          277 t
ORIGIN      
        1 tcgcccgcca tcagccgcgc acctcctcgg cgaacacatg gcgcagacag tcctcggggg
       61 caggggaggg gctctcctcg gcgaaacgca ccgcccgctc gatctcggcc gcgacctcct
      121 cggcgatggc cgccacctcg gcctccgtgg cgattccact cgccgtcaga tgcgcggcca
      181 gtctcgggat cgggtcacgt gccttccatt cggcctcctc ctcccgggtg cggtagccgc
      241 gcgtgtcgtt ccgcgagtgg cccgtgtagc ggtacgtgag gcactccacg agcgtgggcc
      301 cgccaccggt ccgagcccgt tccacgaccg gcaccatcgc ctcatacacg gcctgcacat
      361 ccatgccgtc aaccgtgatg cccgggaacc cgtagccggc agcgcggtcc gaaaggcgct
      421 cgagcttcac cacctcggcg atgggcgtcg aagcgccgta gaggttgttc tcgcacacga
      481 agatgacggg caggtcccag atggccgcca tgttcgccgc ctcgtggaaa gcgccctcat
      541 tgagtgcccc gtcgcctgtg aagcacgcca ccacctggcc cgtggcctgc atcctgaacc
      601 cgagcgccat gccggcggcc accggcgcgt tcccggccac gatggcgatg gccgtgagtg
      661 cgcccacggc ggggtcaccc atgtgcatcg atccgccgta gccgcccgcg cagcccgtgg
      721 tccgtgcgaa cagctcggcc atcatcgagt ccatcgatac gcccttggcg atggcgtggc
      781 cgtgggcccg atgtgtgctg gtgatccagt cggtgggctt cagcgcctcg cagacgccga
      841 cggcgcaggc ctcctggccg gcatagagat gcaacgtgcc cggtagccgg ccctcgagga
      901 acagccggct gacggtctcc tcgaactgcc ggatgcgcac catctggcgg tacatccgga
      961 gtagtggatc gccggggggt tggggcatgg cagtcctcct tcgcgcaagc tcggaccggg
     1021 tagggttcta cccgccttcg gctggtcccc gtgctcggcc catgcgaaca ccctccgcag
     1081 accgaggagg ggccggatga aaggggcccg cgaggccgca agggcgccat ccggcccgcc
     1141 ctgtcaccgg gctacagctc gcatgaacgc ccgcacctcc tctgccgtcg gcaccccggc
     1201 gctgcacccg atggcctgaa tgcagtgggc cgcggtggcg ttggcgaagc aggccgactc
     1261 atcggcgccc cagccctgca agacgccgta caggtaaccc gcaaagaaag catcgcccgc
     1321 acccgagccg tcgaccacgc cgacctcgaa ggcggcgtgg cgccgcgtct cgccgctgga
     1381 gcggatcagg cacccctcgg ccccgagctt cactccgagg gtgcgggtgc cgtgcccgtc
     1441 cagcgtgtcc aggatgcggc ccgggtccga ctcgcctgtg atggcctggg cctcctcgat
     1501 gctcggaatg aacacatcga ggtgcgggag gcagggctcg atgagtgctg cggcgtccac
     1561 gttcgggttg aaggccgtgt ccatcgatgt agtcgcgccg agctgccgcg ctcgggccag
     1621 cagacgcacc gtgggctcgc cgtccatggc gctcgccggc gtcgccgaga aggagacgta
     1681 ctggttcagc ggcgtgtgct tgttgcgggt cgccgtcttg atcgcggtga tgatctggcg
     1741 cgtgatgcag agctccgcga agttgcggaa gctgga
//
LOCUS       DAOSPL010001125         1776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_66829_length_1776_cov_3.039512, whole genome shotgun sequence.
ACCESSION   DAOSPL010001125 DAOSPL010000000
VERSION     DAOSPL010001125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66829_length_1776_cov_3.039512"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLQ54_17395"
     CDS             <1..229
                     /locus_tag="PLQ54_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95093.1"
                     /translation="VNIDDARLVGEIARELDASGRVVPGGIGIYGLRDPNDPRHPTDA
                     NLHIDIRGRSVRWARYYPDRARHDRWQDWPW"
     gene            complement(261..461)
                     /locus_tag="PLQ54_17400"
     CDS             complement(261..461)
                     /locus_tag="PLQ54_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOS95094.1"
                     /translation="MRQGTVKWFSDARGYGFVTLDDGAEVFVHYSAIAVDGFKSLAEG
                     QRVELRVESSPKGPIGTRVRPL"
     gene            complement(549..>1776)
                     /locus_tag="PLQ54_17405"
     CDS             complement(549..>1776)
                     /locus_tag="PLQ54_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOS95095.1"
                     /translation="VHGEPLGEEGVRAAKDALGWPQEPAFYVPDDALAHMRKAVERGA
                     AAQAEWRSRFEAYAAAHADLAREFELYGRGQLPEGWDAGLEELFPPDAPPNATRNTSG
                     KVLNVLAKRIPVLMGGSADLAPSNKTLIDGAGDFGASTPSGRNLHFGVREHAMGSIAG
                     GMALHGGVIPYTGTFLTFADYMRPPMRLAALMKQRVIYVFTHDSIGLGEDGPTHQPVE
                     QTMNLRAVPNLTVIRPADATETAEAWRAAIANTTGPTALLLTRQNLPTIDRTQYAPAA
                     GVLRGGYVLWDSRPGQPEVILIGTGSELELAMKAADALAAEGIGVRVVSMPSWELFDA
                     QDAEYRESVLPAAVRARVAVEAGLRLGWERYVGLDGEVVGMDGFGASAPYQKLYEAFG
                     ITAEAIVERAKALLAS"
BASE COUNT          278 a          636 c          594 g          268 t
ORIGIN      
        1 ggtgaacatc gacgacgcca ggctcgtcgg cgagatcgcc cgggaactcg atgcctccgg
       61 ccgcgtggta cccggcggca tcggcatcta cggactccga gaccccaacg accctcggca
      121 cccgaccgat gcgaacctac acatcgacat ccggggcaga tcggtgcgct gggcgcggta
      181 ctaccccgac cgagctcggc acgaccgctg gcaggactgg ccgtggtagc cggcactggc
      241 ccacgtgcgg cgccacccgg ctagagcgga cggacacgcg tgcctatggg gcccttgggg
      301 ctcgactcga cccgcagttc gacccgctgt ccctcggcca gcgacttgaa cccgtcgacg
      361 gcgatggctg agtagtggac gaacacctcg gcaccgtcgt ccagggtaac gaaaccatag
      421 ccccgggcgt cgctgaacca cttcaccgtt ccttggcgca aggcgtgaac ccccagtagt
      481 cccggcccgc agccaggccg gaccgaaggg gcggcccgaa ggggcgcgcc ccccggttcc
      541 gggtgctcct atgatgcgag cagcgccttc gcgcgctcga cgatggcctc ggcggtgata
      601 ccgaaggcct cgtagagctt ctggtatggg gcactggcac caaacccgtc catcccgacg
      661 acctcgccat ccaggccaac gtagcgctcc cagccgaggc gcaggcccgc ctccacggcc
      721 actcgagcgc gcaccgccgc cgggagaacg ctctcgcggt actcggcgtc ctgggcgtcg
      781 aacagctccc aactcggcat cgagaccacg cggactccga tgccctccgc cgccagtgca
      841 tccgcagcct tcatggccag ctccagctcg gaaccggtgc caatgaggat gacctccggc
      901 tggccgggac gcgagtccca caacacgtag ccgcccctga ggacgccggc ggccggcgcg
      961 tactgggtcc gatcgatggt gggcaggttc tgccgagtga gaaggagcgc ggtgggcccc
     1021 gtcgtgttcg caatggctgc tcgccacgcc tccgcggtct ccgtggcgtc ggccgggcgg
     1081 atcaccgtca ggttcggcac agcccgcagg ttcatggtct gctccacagg ctggtgtgtg
     1141 ggaccgtcct cccccaaacc gatgctgtcg tgggtgaaca cgtagatgac acgctgcttc
     1201 atgagtgcgg ccagccgcat ggggggtctc atgtagtcgg cgaaggtgag gaacgtgccc
     1261 gtgtagggga tgacaccgcc atggagtgcc atgccgcccg cgatggaccc catcgcgtgc
     1321 tcccggactc cgaagtgcag gttgcggccg ctgggtgtgc tcgccccgaa gtcccccgcg
     1381 ccgtcgatca gcgtcttgtt actgggggcc agatcggccg agccacccat cagcaccggg
     1441 atacgtttcg cgagaacgtt gagcaccttc cccgacgtgt tgcgggtggc attgggtggg
     1501 gcatcgggtg ggaagagctc ttcgaggccc gcatcccagc cctcgggcag ctgccctcgg
     1561 ccgtagagct cgaactcgcg cgccaggtcg gcgtgagcgg ccgcgtaggc ctcgaagcgt
     1621 gaacgccact cggcctgggc ggcggcaccc cgttcgacgg ccttcctcat gtgggccagc
     1681 gcatcgtctg gcacgtagaa cgccggttcc tgcggccagc cgagggcgtc cttggccgcc
     1741 cggaccccct cctcgcccaa gggctcgcca tggacc
//
LOCUS       DAOSPL010001126         1775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_66895_length_1775_cov_2.557558, whole genome shotgun sequence.
ACCESSION   DAOSPL010001126 DAOSPL010000000
VERSION     DAOSPL010001126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66895_length_1775_cov_2.557558"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PLQ54_17410"
     CDS             complement(<1..662)
                     /locus_tag="PLQ54_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOS95096.1"
                     /translation="MIVGVPTEIKDGEQRVAITPDGVATLTSDGHRVLVQAGAGAGAG
                     IDDDQFASAGAVTVPSAEEVWGTSDLVLKVKEPVPQEYPLLRRGQVLFTYLHLAAGRE
                     LTLALLESGIVAIGYETVQLDNGTLPLLVPMSEVAGRLAVQAGARALEAHSGGRGILL
                     GGVPGVAPADVAIIGAGVVGSNACTIAMGMRANVTVLDKSHDRLRYVQDTMHGNVITV
                     IAT"
     gene            835..1371
                     /locus_tag="PLQ54_17415"
     CDS             835..1371
                     /locus_tag="PLQ54_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95097.1"
                     /translation="MPLVILFGLASSATAAPPGAFAIHSATDIGHEFSFYFDGRFGAQ
                     YVEPQGGADCRVWGTLSKFDLGNVNLLVLSSGATPCAYTPEDVAAVRAYLEAGGGVVV
                     LGDFATFRGEEAYRLNDLAAAFGARFVPDAAEVPLRAAKELEAGTIDYYGGRVLELTE
                     PGAWHVLIEDAARRAGHQ"
BASE COUNT          281 a          620 c          554 g          320 t
ORIGIN      
        1 gtggcgatca ccgtgatcac attgccgtgc atcgtatcct gcacatacct cagtcgatcg
       61 tggctcttgt cgagcacggt gacgttggcc cgcatgccca tggcgatggt gcacgcgttg
      121 gagcccacca cgcccgcgcc gatgatggcc acgtcggccg gcgccacgcc cggcacgccg
      181 cccaacagaa tcccccggcc gccgctatgt gcttccagcg ccctggcccc ggcctggact
      241 gccagacggc cggccacctc actcatgggc accagcagtg gcagcgttcc gttgtcgagc
      301 tgcacggtct cgtacccaat cgcgacgatg cccgactcga gcaaggccag cgtcagctcg
      361 cgcccggcgg ccaggtgcag gtaggtgaac agcacctggc cgcgccggag aaggggatac
      421 tcctgaggca cgggctcctt gactttgagc accaggtccg acgtgcccca cacctcttcg
      481 gccgacggca cggtcacggc cccagccgaa gcgaactggt catcgtcgat tcccgcgcca
      541 gcgccggcac ccgcctggac gaggacgcgg tggccgtcgc tcgtgagcgt cgcgacccca
      601 tcgggcgtga tcgccacgcg ctgctcgcca tctttgatct cggttgggac ccctacgatc
      661 acgcggcatc accttcctgt gtgtgtcaag caagcattcc ctttcaaacg cgcgtcatcc
      721 agccccttca cgttccgacg ccacgtgggc tgctcgaccg cttgccggag gcagtgccgc
      781 gccgtacact cgaccatgtc gtcgttccat cgcctctcga gcggggagtc tgccatgccc
      841 ctcgtgatcc tgttcggtct cgccagctcc gccacggccg cgccgcccgg cgcattcgcc
      901 atccactcgg ccacggatat cgggcatgag ttcagcttct acttcgatgg gcggttcgga
      961 gcccagtatg tggaacccca aggcggcgct gattgccggg tctggggcac actcagcaag
     1021 ttcgacttgg gcaacgttaa tctcttggtg ttgagctcgg gcgccacgcc ctgtgcctac
     1081 acgcctgagg acgtggcggc cgtgagggcg tatcttgagg caggcggagg cgtggtggtc
     1141 ctcggcgatt tcgccacatt ccgcggcgag gaagcttacc gcctgaacga cttggcggcg
     1201 gcctttgggg cccggttcgt gcccgatgcg gccgaggtgc ccctgcgcgc agccaaggag
     1261 ctcgaagcgg gcaccattga ctattacggg ggccgcgttc tcgagctgac tgagcccggc
     1321 gcgtggcatg tactcatcga ggacgccgcc cggcgagccg gtcaccagtg aacaacaccg
     1381 actcctctca caatcgcccc caaaaagagg cgcagaagtc cagggatgct cagcactcac
     1441 ggttcgcccg gtgacccgta gtctcgtgcc atggcaacgg tcgacccggt caatgttgtt
     1501 cggttcgtgc tcggtggtcg agcctacgaa ctgcgtcgct ccgatgttga ggcgcggctc
     1561 gctggcgtgc gcccccgatc gatcagcacg cacgctgtcc tgatcaatgg ggactggtat
     1621 ccggtccggc aggctttcga ggttgcgact ggagtaccag cggatctctt caactcgcac
     1681 acggcccgtc gccatctggc gagtctgggc ttcgagattc gcggcgacat ccggcaccgt
     1741 gaccgtcaac ggctacgaca tcctggacaa cccgc
//
LOCUS       DAOSPL010001127         1774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_66943_length_1774_cov_2.963351, whole genome shotgun sequence.
ACCESSION   DAOSPL010001127 DAOSPL010000000
VERSION     DAOSPL010001127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66943_length_1774_cov_2.963351"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            395..580
                     /locus_tag="PLQ54_17420"
     CDS             395..580
                     /locus_tag="PLQ54_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOS95098.1"
                     /translation="MISKELLEILVCPACRGELDYEPDVRLTCRSCGKSYPIRDGIPI
                     MLVDGEGAEPQGGEGDA"
     gene            573..1523
                     /locus_tag="PLQ54_17425"
     CDS             573..1523
                     /locus_tag="PLQ54_17425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOS95099.1"
                     /translation="MPEGGGGASRALTAGSENPVDVSICFVNWRTADDLIGCVRSIEA
                     HPPACSYDVWIVDNDSGDGSVERLRAELPGARLIANSANVGFGRAANQAIASSTGRYV
                     LLLNPDMVVDPGAVDTLVAFMDSEAHAGAIGGAMRAPDGRRHHSCRRFPVPWAALVRG
                     TFLETLLPRDRRVREYLMEDWDHSSVREVDWLSGACLMLRREALDQVGVFDARYFMYL
                     EDTDLCLRMHRSGWGVYYVPSAGFVHTIGRSSDQAQPRMILAHHRSMCQYYCKHMARG
                     LGLLLVPLALAAIGARCLLVLAKNRMLWWRTTRIAGTNKT"
     gene            1544..>1774
                     /locus_tag="PLQ54_17430"
     CDS             1544..>1774
                     /locus_tag="PLQ54_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95100.1"
                     /translation="MAARRPKNALAKGRRPKDRSRRNSWATEIGWWRCLDILVEAPLF
                     LALLLTPVVAGECVAGQPPRGGELSLSLAFCTL"
BASE COUNT          269 a          565 c          625 g          315 t
ORIGIN      
        1 acccggcatg cagagcggtt tccgccagtg ccgcctcctc ggccgctgcc agcgcggcgt
       61 ccggccgggc accgacccat gccgaggggg ctgagccgaa ctgcgtacac gttgttgcgt
      121 ggcgctccag gaccaaggtt gccatccgac tcactcgctc tctctcccgc gcgccggtcc
      181 aggttttggt cgcgcgcgat accgagggag tgtgactgca acgagattgc cagccgtgaa
      241 cggcccaccg gggcgatggg cgggtgaggc tgcccaggac ttggtggaat ccaccaactc
      301 gccgcggtct ccatggatgg caggagggtg gccgtggtgc cctgaacaca gaggcgcccg
      361 gcgcgcccca accggtgcga ggagggaacc gtccgtgatc tccaaggagt tactcgagat
      421 acttgtgtgc cccgcctgcc gtggcgaact ggactatgag cccgatgtga ggctgacctg
      481 ccgaagctgt gggaagtcgt accccatccg cgacggtatc cccatcatgc tggtcgacgg
      541 agagggggcc gagccgcagg gcggcgaggg tgatgcctga gggtggcggc ggagcgtctc
      601 gagccctgac cgctggatcc gagaaccccg tggatgtatc gatctgcttc gtcaactggc
      661 gtaccgctga cgaccttatc ggatgtgtgc gctcgattga ggcccacccc ccggcctgca
      721 gctacgacgt atggatcgtc gacaatgact cgggcgatgg ctccgtcgag cgtctgcggg
      781 cggagctgcc gggggcgcgc ctgatcgcga actccgccaa cgtcggtttc ggccgggccg
      841 ccaaccaggc gatcgcgtcc agcaccggac gttatgtgct tctgctgaac cctgacatgg
      901 tggtggatcc gggcgccgtc gacacactgg tggcgttcat ggactcggaa gcacacgcgg
      961 gggcgatcgg gggggccatg cgtgccccgg acggtcggcg gcatcactcg tgccggcgct
     1021 ttcccgtgcc ctgggccgct ctggtccggg gcacctttct cgagacgctc ctgccgcggg
     1081 atcgccgggt ccgcgagtat ctcatggagg actgggacca ctcctcggtc cgcgaggtcg
     1141 actggttgag cggcgcctgc ctgatgctgc gtcgagaagc gctcgaccaa gtgggtgtct
     1201 ttgacgcacg gtatttcatg tatcttgagg acacggatct atgcctgcgc atgcaccggt
     1261 cgggctgggg tgtctattac gtgccgtcgg cggggttcgt ccacacgatc gggcgcagca
     1321 gtgaccaagc gcagccccgg atgatcctgg ctcatcaccg gagcatgtgc cagtactact
     1381 gcaagcacat ggcccggggt ttgggcctgc tgctggtgcc ccttgccctg gccgcgatcg
     1441 gggcccggtg tctgctcgtt ctcgcaaaga accggatgct gtggtggcgg accacacgga
     1501 ttgccggcac caacaagacg tgaggagacc ccggcccact gatatggcag cccgtagacc
     1561 gaagaacgcg ctggcgaaag gccggcgacc caaggacagg agccggcgca actcgtgggc
     1621 gactgagatc ggctggtggc gttgcctcga tatcctggtc gaggcgccgc tgttcctcgc
     1681 cctgctgttg acgcccgtcg tcgcggggga gtgcgtggcc ggtcagccgc ctcgcggggg
     1741 cgagctgagc ctgagtctgg cgttttgcac cctc
//
LOCUS       DAOSPL010001128         1773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_67008_length_1773_cov_2.603609, whole genome shotgun sequence.
ACCESSION   DAOSPL010001128 DAOSPL010000000
VERSION     DAOSPL010001128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67008_length_1773_cov_2.603609"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..>1773
                     /locus_tag="PLQ54_17435"
     CDS             44..>1773
                     /locus_tag="PLQ54_17435"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOS95101.1"
                     /translation="MTVRGVSGPDAALRNQHYVSNRAPLAPSPFVKLPIGAITPRGWL
                     RHQLELMRDGMTGHLSEISPWCDPRDNAWLSPTGQGRNGWEELPYWLKGFGDLGYVLG
                     DPRIIAEARVWIEGALGSGRPDGYFGPEANRTSLEGKADLWPHMVMLNCLQSYYEATG
                     DPRVIDRMTAFFRYELGVPESEFLLPFWQKIRAGDNLESVYWLYNRTGEPWLLELAEK
                     IHRNTADWVHGIASWHGVNITQGFREPAVYSVLSGQATDREAAERNYQAVMGLYGQVP
                     GGMFGADENAREGFDDPRQGAETCSMVEFMHSFEMLFSMLGDTIWADRCEEVAFNSLP
                     ASQPPDLKGLHYLTGANMVQLDKENKSPGLQNGGCMLAYDPYSYRCCQHNVSHGWPYY
                     AEHLWMATPGDGLAAVLYSASEVTAKVGAAGRSVTVSEETGYPFSGEVRFRLATDEPV
                     EFPLFLRIPHWCKAPRLRINGRTVEAQVQAGAFLRIDGTWRSGDTVTLELPMEVVVRR
                     WERNHGSVSVSRGPLTFSARIGERWARYGGTEQWPAYEVFPTTPWNYGLVLDADKPEA
                     SFAVEETDAVP"
BASE COUNT          288 a          552 c          646 g          287 t
ORIGIN      
        1 ggggatgacc gtgtcggtct cgggactggg ggcgcgtgag gcgatgacgg tacgcggtgt
       61 gtccgggccg gacgcggccc tccggaacca gcactatgtg agcaaccgcg cgccgctggc
      121 gccgagcccg ttcgtgaagc tgcccattgg cgcgatcacc ccccgcggtt ggcttcgcca
      181 tcagctcgag ctgatgcggg atggcatgac cggtcacctc agcgagatca gcccctggtg
      241 cgacccgcgg gacaacgcct ggctcagccc cacgggccag ggcaggaacg ggtgggagga
      301 gcttccctac tggctcaagg ggttcggcga cctgggctac gtgctcggcg acccgcgcat
      361 catcgcggag gccagggtgt ggatcgaggg agcactgggg agcgggcgac ccgatgggta
      421 cttcggccca gaggccaacc ggaccagcct cgaaggcaag gcggatctgt ggccgcacat
      481 ggtgatgctc aactgcctcc agtcgtatta cgaggccact ggcgatccac gcgtcatcga
      541 ccggatgaca gccttcttcc gctacgagct cggggtgccc gagtccgagt tcctgctgcc
      601 cttctggcag aagatccgcg ctggcgacaa cctggagagt gtgtactggc tctacaaccg
      661 cacgggtgag ccgtggctgc tcgagctggc cgagaagatc caccggaaca cggcggactg
      721 ggttcacggg atcgcctcgt ggcatggcgt gaacatcacc cagggcttcc gggagccggc
      781 cgtctactct gtgctgtcgg gccaggcgac ggaccgggag gccgcggagc gcaactacca
      841 agcggtcatg ggcctgtacg gccaggtgcc ggggggcatg ttcggagccg acgagaacgc
      901 gagggagggt ttcgacgatc cccgccaggg cgcggagacg tgctcgatgg tcgagttcat
      961 gcacagcttc gagatgctct tctcgatgct cggtgacacc atctgggccg accggtgtga
     1021 ggaggtcgcg ttcaactcac tgccggcgtc gcagccgccg gacctgaagg ggctgcacta
     1081 cctcacgggg gccaacatgg tgcagctcga caaggagaac aagagccccg gcctacagaa
     1141 cggcggctgc atgctggcct acgaccctta cagctaccgc tgctgccagc acaacgtgag
     1201 ccatggttgg ccctattatg ccgagcattt gtggatggcc acgccgggcg acgggctggc
     1261 ggcggtgctc tactcggcct cggaggtcac ggccaaggtg ggtgcggcgg gccggtctgt
     1321 gaccgtgtcc gaggagaccg gctatccgtt ctcgggcgag gtgcgattcc ggctggccac
     1381 ggacgagccg gtcgagttcc cgctgttcct ccgtatcccg cactggtgca aggcaccgcg
     1441 gcttcggatc aacggccgga ccgtggaggc gcaggtccag gcgggcgcct ttctgcgcat
     1501 cgacggcact tggcgctcgg gcgacacggt gaccctcgag ctgccgatgg aggtcgtggt
     1561 gagacggtgg gagaggaacc atgggagtgt ctcggtgagc cgtgggccgc tcaccttctc
     1621 ggcgcggatc ggcgagcggt gggcgcggta tggtggcacg gagcagtggc cggcctacga
     1681 agtgttcccg accacgccct ggaactacgg tctggtgctc gatgccgaca agcccgaggc
     1741 gagcttcgcc gtcgaggaga ccgacgcggt tcc
//
LOCUS       DAOSPL010001129         1770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_67145_length_1770_cov_3.524198, whole genome shotgun sequence.
ACCESSION   DAOSPL010001129 DAOSPL010000000
VERSION     DAOSPL010001129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67145_length_1770_cov_3.524198"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PLQ54_17440"
     CDS             complement(<1..865)
                     /locus_tag="PLQ54_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HOS95102.1"
                     /translation="MAKIAFIGAGSFGFTRTLVKDILTFPLLEDAELALMDIDEERLE
                     YSTRAVNRIVKEGGYRAKVTATTDRRKALQGADAVIITILAGGVQVWRHDIEIPKKFG
                     VDTNVGDTRGPSGIFRALRTIPVMVSICRDMEELCPRALLLNYTNPMAMLCRAMQRES
                     RITVSGLCHSVQGTAAMLAHWIGAPYDEIQYVCAGINHLSWFVKFEWNGEDAYPLIRQ
                     AMKRKAVYNEEKVRNELFLALGYYVTESSGHNSEYNWWFRKRQDLIDKYCLDGTGWNP
                     GEYAYILNEYLK"
     gene            complement(930..>1770)
                     /locus_tag="PLQ54_17445"
     CDS             complement(930..>1770)
                     /locus_tag="PLQ54_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95103.1"
                     /translation="VELIATLDVGPRIIRFGFTGDRNEFAEFAEDMGKTGGDEWRLYG
                     GHRLWHAPEEKPRTYFPDNAPVAVREVDGGVELTPPVETTTGIQKRISVLLDGDANRV
                     RVIHGITNTNLWAVSLAPWSLSVMAPGGHAIIPHEGFRPHTEVLLPARPLVLWYYTDM
                     SDPRWTWGRQYIQLQQDPGNPAPQKLGAMIRAGWAAYSNEDHLFIKRFPFVEAGAYLD
                     YGCNAEFFTNERMLEVESLGVRVTLEPGQTVEHEEVWTLHRGLPELRTDAQIEANVLP
                     LVR"
BASE COUNT          310 a          605 c          519 g          336 t
ORIGIN      
        1 tcttgaggta ctcgttgagg atgtacgcat actcgcccgg gttccagccc gtaccgtcga
       61 gacagtactt gtcgatcaag tcctgacgct tgcggaacca ccagttgtac tccgagttgt
      121 gcccgctgga ctctgtcaca tagtacccca gggcgaggaa gagctcgttg cgcaccttct
      181 cctcattata tacagccttg cgcttcatgg cctgccggat cagtgggtac gcgtcctcgc
      241 cgttccactc gaacttgaca aaccacgaga ggtggttgat gcccgcgcag acgtactgga
      301 tctcatcgta gggtgcgccg atccagtgag ccagcatggc tgccgtgccc tgcacgctat
      361 ggcacagccc gctgacggtg atccggctct ctcgctgcat ggcccggcac agcatggcca
      421 tggggttcgt gtagttgagc agcaaggcgc gcgggcagag ctcctccatg tccctgcata
      481 tactcaccat cacgggaatg gtccgcaacg cccggaagat gcccgatggc ccgcgagtgt
      541 cccccacatt ggtgtcgacc ccgaacttct tggggatctc gatgtcgtgc cgccacacct
      601 gcacgccgcc cgcgaggatc gtgatgatga ccgcgtccgc gccctgaagg gccttccggc
      661 ggtccgtggt cgccgtgacc ttggcccggt agcccccctc tttcacgatc cggtttaccg
      721 cacgcgtgct gtactccagg cgctcctcgt caatgtccat gagcgccagt tcagcatcct
      781 caagcaatgg gaaggtgaga atgtccttca ccagggtgcg cgtgaaaccg aagctccccg
      841 cgccgatgaa cgcgatcttc gccacgatcc tgtctccttc agttcgggtc agtcccgcaa
      901 gacctcagcc tcgccgggat cggctggtgt caacgcacca gcgggaggac gttcgcctcg
      961 atctgggcat cggtccggag ctccggtagt ccgcgatgca gggtccagac ctcctcgtgc
     1021 tcaacggtct gccccggctc cagggtcacc cgcacgccga ggctctccac ctcgagcatc
     1081 cgctcattcg tgaagaactc ggcgttacag ccatagtcca agtacgcgcc cgcttccacg
     1141 aagggaaagc gcttgatgaa cagatgatcc tcattcgagt aggcggccca gccggcccgg
     1201 atcatcgcgc caagcttctg cggcgccgga ttccccgggt cctgctggag ctggatgtac
     1261 tggcggcccc aggtccatcg cgggtcgctc atatccgtgt aataccacaa gaccagaggc
     1321 cgggccggca acagcacctc ggtatgggga cggaacccct cgtgcgggat gatggcatgc
     1381 ccgccgggtg ccatgaccga cagcgaccac ggcgccagac tgaccgccca gaggttcgtg
     1441 ttggtgatgc cgtggatcac ccgcacgcgg ttcgcgtccc cgtccaacag caccgagatc
     1501 cgcttctgga tcccagtggt ggtctccacc ggcggagtca gctcgactcc accatcgacc
     1561 tcccgcacgg caaccggcgc gttatcggga aagtacgtgc gcggcttctc ctccggagcg
     1621 tgccaaaggc ggtggccccc atacagccgc cactcgtcgc caccggtctt gcccatgtcc
     1681 tccgcgaact cggcgaactc gttgcggtcg cccgtgaatc cgaaccggat gatgcgtgga
     1741 ccaacatcga gtgtcgcgat aagctctacc
//
LOCUS       DAOSPL010001130         1770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_67151_length_1770_cov_3.123615, whole genome shotgun sequence.
ACCESSION   DAOSPL010001130 DAOSPL010000000
VERSION     DAOSPL010001130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67151_length_1770_cov_3.123615"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(793..>1770)
                     /locus_tag="PLQ54_17450"
     CDS             complement(793..>1770)
                     /locus_tag="PLQ54_17450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95104.1"
                     /translation="VYQARRQLTAAVRQALTSGRGPDESEEPLTSRAALLGALSGMAI
                     VITMPIVAGVAWWVSLMYFGLMFVVLLVYCRNRAEMGFPIVWGYPLYQQRQSMVDFLG
                     SAAFVSPGRTRSFTLLTMFSWLQRSVNQAITSTGQEAYVAADRLGLGRRTATGVVMAA
                     LAFGIVTAFLANLSAYYEYGGLVLSSSGGIEGGQMTQEVLGQFTMVSRWIDEPVPPHR
                     EYIGFTVGGAVLALGMILGRRTWLRFPFHPAGYALAFSHDGSYMWFPALLLWAMKGIT
                     VRLGGVRLYRRLSRGFLAFTLGHFLSVGVWSLAGLHAPEWVRQYIVWFL"
BASE COUNT          314 a          649 c          587 g          220 t
ORIGIN      
        1 cgaagctggc ggggcatggc atagaggtct gcagccgcta cgatcgccgt ggcgacgagg
       61 ccaagaggaa ggccgtggcc ctgctccatg tcccctaccg ccgcagggcg gccctgccgc
      121 cggactgagc gtgggtccca tagggactcc gcactcgatt cccggcgagg gggggtgtgg
      181 gtagaaggaa gaggacccga cgcaacccct gtgtcgacgc cggtggacct tttcggtggg
      241 ccccaggatc gacccacgtg gggccccgct ccctcgcggc gtccgtcagg acgccgcgag
      301 gggggcctcc acggccgccc taccgagcac cttggagatc cacgccctcg aacagcccag
      361 cctcctcgcc agctcggccc ggttgcggac ctcgccggat tcgatcatcc gccggaaccg
      421 ctcgacccgc tccgccgggc actccctggg ccgtgcggcc cgccgcacgg cgggcggcgg
      481 gtaggtcctg ccgaacctca cccgcatcgg gcagcggagg gcccaacgca tccgacgctg
      541 gctcgtgaca gcggagcccc aacccgcacc acccaagccc ccgccccggc gggggcctct
      601 tcgtgtgtgc ccccaccagc cgacccgcgc ccttcccgct gcgcgggccc cctccatacg
      661 gcccctcctc ggtggctcca atgcctttcg gaggggccga atagggggaa ccagccgcag
      721 gctggtggga ccctatccgg cccgcagttc aggcgtgcca ccccggggac acgggtaaca
      781 cctggatccg ggctagagga accacacaat gtactggcgg acccactcgg gggcatggag
      841 gccagccaaa ctccagactc ccaccgacag gaagtgcccg agggtgaacg cgaggaagcc
      901 tcgcgacagg cggcggtaga gccgaacgcc cccgaggcga acggtgatgc ccttcatggc
      961 ccacagcagc agggcgggaa accacatgta cgagccgtca tggctgaagg cgagggcata
     1021 gcccgccggg tggaagggga accggagcca tgtgcgtcgc ccgaggatca tccccagggc
     1081 cagaacggcg ccgcccacgg tgaagccgat gtactcccgg tggggcggca ccggctcatc
     1141 gatccacctc gagaccatgg tgaactggcc caagacctcc tgggtcatct ggcccccctc
     1201 gatgccaccc gagctggaga gcaccaggcc tccgtactcg taataggccg acaggttagc
     1261 cagaaaggcg gtgacgatgc cgaaggcgag cgccgccatg accactccgg tggccgtgcg
     1321 ccggccgagc cccaagcggt ccgccgccac atacgcctcc tgacccgtgc tggtgatggc
     1381 ctggttcacg ctgcgctgga gccatgagaa catggtcagc agcgtgaacg accgtgtccg
     1441 gcccggcgac acgaaggccg cgctgcccag gaagtccacc atggactgcc gctgctggta
     1501 gagcgggtac ccccacacga tcgggaagcc catctcggcc cggttccggc agtagaccag
     1561 gagcaccacg aacatgagcc cgaagtacat cagcgagacc caccacgcca caccggccac
     1621 gatgggcatg gtaatgacaa tggccatgcc ggacagagcg ccgagcagcg cggcccgcga
     1681 tgtcagtggc tcctcggact cgtccggccc ccggccactc gtcagagcct ggcggaccgc
     1741 cgccgtcagc tgtcgccgag cctggtagac
//
LOCUS       DAOSPL010001131         1769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_67217_length_1769_cov_2.451575, whole genome shotgun sequence.
ACCESSION   DAOSPL010001131 DAOSPL010000000
VERSION     DAOSPL010001131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67217_length_1769_cov_2.451575"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PLQ54_17455"
     CDS             complement(<1..512)
                     /locus_tag="PLQ54_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95105.1"
                     /translation="MADEFTFARRASGLVRGLDMWDAFGAGLMTCQPIATIWMMVGIA
                     LSLFPGANLVVAIILGALTAGIGAPLVWGILSGSMPRAGGEYIYNSRILHPAIALAAV
                     FTNIVAICYWNWFIATWLGVPALQVFGQYMGWQGFSDWAASKTGLCILGLVVVVVGYL
                     SVAFSMKSYA"
     gene            797..>1769
                     /locus_tag="PLQ54_17460"
     CDS             797..>1769
                     /locus_tag="PLQ54_17460"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOS95106.1"
                     /translation="MNLADEDWFATAGEARPGLRAETPGLRDEQTASVLGALSPTARR
                     ILRAAFRVLERDGYDGLSLRRIAAEAGETRSLIAYHFENKAGLVATLVDSLWHDADVA
                     LEHEVEGLAADPRGRLSALTGLHLRLARQPNLYRTYFDLLPHILRDGGACSRLARTYR
                     SYRRIGELCLAPCVGRDVDALALATLLLAVGEGLGAQVLLGGGDEPAVAAFALLEREV
                     AAYLGLGPVLDGQAPGPGAAGAAAVEGELSPELPDPAAGLPPAAGRVLRAALALLDDV
                     GPQALTAEALARKSGEPSSSVFYYFGDKHGLLTAVLAAEQYRFVQALT"
BASE COUNT          278 a          603 c          605 g          283 t
ORIGIN      
        1 atcgcgtagc tcttcatgct gaaagccacc gaaaggtagc cgaccacgac gacgaccagc
       61 ccaaggatgc aaagcccggt cttcgacgcg gcccagtcac tgaacccctg ccaacccatg
      121 tactgaccga agacctgcag agccgggact ccaagccagg tcgcgatgaa ccagttccag
      181 tagcagattg cgactatgtt ggtgaacacg gctgccaagg cgatcgcggg atgaaggatg
      241 cgcgagttgt agatgtactc gccgcccgcc cgcggcatcg agccgctgag gatcccccat
      301 acgagagggg ctcctatccc ggccgtcaga gcgccgagga tgatcgcaac gacaaggttg
      361 gcgccgggaa acagcgacag cgcgatgccc accatcatcc agatggtggc gatcggctga
      421 catgtcatga gacccgcgcc gaatgcgtcc cacatgtcga gaccgcgtac caagccgctg
      481 gcccggcgtg cgaaggtgaa ctcatcagcc atatggatcc ccctggtcgc tggtgctgac
      541 tgctctgtac gtgcggaagc gacgctgcag tcgcccggag ggcgcaagac cgacaggacc
      601 gctgccgcgt cgaccctcgc gctcactccc gctcactcca tcggcggcct ccgtccttcg
      661 ctgcgtgtcg aagctctggc tgaccggtca accagaggtc gcctatacta caagtcggcg
      721 ggtcggtgtc aaggaacgtg gacgaagcgg acagagtggc tggttcagtg gagccgggag
      781 atttgagagg catggcttga acctggcgga cgaagactgg ttcgcgaccg caggcgaagc
      841 gcgccccggt ctgcgcgccg agacgccggg cctgcgtgac gagcagacgg catccgtcct
      901 cggcgcgctg tccccgacgg cgaggcgcat cctcagggcg gcgttccggg tgctcgagcg
      961 ggacggttac gacggccttt cgctgcgccg gatcgccgcc gaggccggcg agactcgctc
     1021 actcatcgcc taccacttcg agaacaaggc tgggttggtg gcgacccttg tcgactcgct
     1081 atggcatgat gccgacgtgg ccctcgaaca cgaggtcgag gggttggcgg cggacccccg
     1141 gggccgcctg tcggccctta ccgggctgca ccttcgtctg gcccggcagc ccaatctcta
     1201 ccgcacgtat ttcgacctgc tcccgcacat cctccgcgac ggcggtgcat gctcgcggct
     1261 ggcgaggacc tatcggtcgt accggcgcat cggcgagttg tgcctggcgc cgtgcgttgg
     1321 ccgcgacgtc gatgcgctcg cgttggcgac cctgctgctg gccgtgggcg aggggctggg
     1381 cgcgcaggtc ctcctcggcg gcggcgacga gccggcggtc gcggccttcg cccttctcga
     1441 gcgcgaggtc gccgcgtacc tcgggcttgg cccagtgctc gatgggcagg cgcctggacc
     1501 gggcgccgca ggcgccgcgg ccgtggaggg cgagctgtcg ccggagctgc ccgacccggc
     1561 cgccggcctt ccgccggcgg ccgggcgtgt gctccgcgcg gcgctcgccc tcctggacga
     1621 cgtggggccg caggcgctca ccgcggaggc gctcgccagg aagtccggcg agccctcgtc
     1681 gtcggtcttc tactacttcg gcgacaagca tggactgctc accgccgtgc tggctgctga
     1741 gcagtatcgt ttcgttcagg cgctcacac
//
LOCUS       DAOSPL010001132         1768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_67245_length_1768_cov_3.430823, whole genome shotgun sequence.
ACCESSION   DAOSPL010001132 DAOSPL010000000
VERSION     DAOSPL010001132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67245_length_1768_cov_3.430823"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /gene="nadA"
                     /locus_tag="PLQ54_17465"
     CDS             complement(<1..649)
                     /gene="nadA"
                     /locus_tag="PLQ54_17465"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOS95107.1"
                     /translation="MRLPSELRAAEYPLLADRILRFKQQRNAIILVHNYQLPEVQDIA
                     DVLGDSLGLSQHAARTDADVIVFCGVHFMAETAAVLCPDKIVLLPEPESGCPMADMIT
                     PPQLEALKAAHPGVPVITYVNSSAEIKAMSDYCCTSANAVRVLQAVDADEVLFVPDRW
                     LASYAARFTDKKVHWHEGFCPTHHRILPEHIERLKHDHPDAVVVCHPECRADVVDM"
     gene            complement(680..1033)
                     /locus_tag="PLQ54_17470"
     CDS             complement(680..1033)
                     /locus_tag="PLQ54_17470"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOS95108.1"
                     /translation="MLRSMCKSKIHRATVTDAALHYEGSLTLDRDLMDAADLLEHERV
                     QVVNVNTGDRFDTYIIEGPRGSGAVCLNGAAARLGHPGDLVIIMSFAYYTEDEARSHT
                     PRVVRVDTANRPVAG"
     gene            1617..>1768
                     /locus_tag="PLQ54_17475"
     CDS             1617..>1768
                     /locus_tag="PLQ54_17475"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOS95109.1"
                     /translation="MDEGLQRFGVSMDSRLLSAFDDRIQAAGYTNRSEALRDMVRDYL
                     VEHEWRS"
BASE COUNT          316 a          582 c          580 g          290 t
ORIGIN      
        1 ccatatcgac cacgtcggca cggcactcgg ggtggcagac gacaaccgca tcgggatggt
       61 cgtgcttcag ccgctcaatg tgctcgggca agatgcggtg gtgggtgggg cagaacccct
      121 cgtgccagtg caccttcttg tcggtgaacc gggcggcgta cgaagccagc cagcggtcgg
      181 ggacgaagag gacctcgtcg gcatcgacgg cctgcaagac tcggaccgcg ttggccgacg
      241 tacagcagta gtcggacatg gccttgatct cggccgagga gttcacatag gtgataacgg
      301 gcacacccgg atgcgccgcc ttgagggctt ccagctgcgg cggagtgatc atgtcagcca
      361 tggggcagcc ggactcgggc tcaggaagaa gcacgatctt gtccgggcac agaacggccg
      421 ccgtctcagc catgaagtgg acgccgcaga acacaatgac atcggcatcg gttcgcgcgg
      481 cgtgttggct caggcccagg ctatcgccca gaacgtctgc gatatcctgc acctcgggta
      541 gttgataatt gtggacgagt atgatggcat tccgctgctg cttgaaccgc aggatgcggt
      601 ccgctagcag cgggtactca gcagcacgga gctccgacgg gagcctcatc gcgtgcgact
      661 ccttgcgggc gccccgaagt cacccggcga cggggcggtt ggcggtatca acgcggacca
      721 cacggggcgt gtgagagcgg gcctcgtcct ccgtgtagta ggcaaaactc atgatgatga
      781 ccaggtcgcc tgggtgtccg agccgggccg ctgccccatt caggcacacg gctcccgaac
      841 cacgcggccc ctcgatgatg tacgtatcga accggtcacc cgtgttcaca ttgacgacct
      901 gcacgcgttc gtgctccagc aggtcggcgg catccatgag atcccggtcc agcgtgaggc
      961 taccctcgta gtggagagcg gcatccgtga ctgtggcccg atggatcttc gacttgcaca
     1021 ttgaccgcaa catgatgact ccgctgccaa atgcccggag acgaagaagg gccccatggg
     1081 ggccccgaac cctgactctc ctagtgcatc gattctacgg ggtggccctc cccaccgtca
     1141 acccgctccc ccagccggac cgctcctgac cgccctctct gtgggcaagc cagaggtgcg
     1201 gactgaactc cgttcaccat gccatggcgc cagctgccca ccgtccggcc tcatgcggaa
     1261 cgaaatcccc tccaagcggg tctcgcgcat gggccggctg cgtggtgatg gtactgcctc
     1321 tagcgtcgcg aacacgacgg ctcccacgaa cggctgccgc tcagtgaggg cccgccgccc
     1381 cgccgtcccg gttggtccgg gcgggcacag cagtagccca cggctgaaga gctgagctcg
     1441 gcgcaccagt cggtagccgg cagtccacgg tacggggccg acgactggat cgcccgagcg
     1501 gccggggaat cgggcgccaa cccgagtccc aagcccccgg gcgtttgcgc cgtggcggga
     1561 atccccgacc agtgcgagcg tagcaccctg gcgcaattcc gtgctacact gggcacatgg
     1621 atgagggact gcagcgcttc ggtgtctcga tggactctcg gctgctcagc gccttcgacg
     1681 accggatcca ggcggcaggc tacaccaacc ggtcggaggc gctccgcgac atggtgcgcg
     1741 actacctggt cgagcatgaa tggcgctc
//
LOCUS       DAOSPL010001133         1767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_67311_length_1767_cov_3.276869, whole genome shotgun sequence.
ACCESSION   DAOSPL010001133 DAOSPL010000000
VERSION     DAOSPL010001133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67311_length_1767_cov_3.276869"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="PLQ54_17480"
     CDS             <1..1258
                     /locus_tag="PLQ54_17480"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOS95110.1"
                     /translation="VICLAACLLLGPGKLGVALPTSAHLVVLYTNDTRGELDPCECSE
                     LTGGLARRATLVEQIRAEGFPVLLVDSGDIVGRPAKLQPMLDAMQRIGYQHVGLGGVD
                     GLLRDAFVRAAAERGLTVHSLAMGEGVAPPDVVVVEGFRVGIVGVGSDHLGLPASKVC
                     ARVSTAVADLESRADLVLILSQMGRKIDRAIASRLSRGSTPVVVVGNRVWHDLVEPEN
                     IGRVIIVPTGYLGRWLGRLDVTRLMGATRVEAKLIPVAPTLKQQPDVQRIVKAYYDRE
                     RAAAKPAPVPTKAATLTGDPSDCIQCHRTQFDTWLTQKHSRAVATLVAKDKAIPECLM
                     CHSQVYRQERRVPSSEDIDDGVGCLVCHSPAVGHTGEERPFSVRRAAGQAQCIQCHTP
                     ERDPDFDYAAALERIRHWDEPVAIAR"
     gene            1266..>1767
                     /gene="ribD"
                     /locus_tag="PLQ54_17485"
     CDS             1266..>1767
                     /gene="ribD"
                     /locus_tag="PLQ54_17485"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOS95111.1"
                     /translation="MRRALELAALARGFTSPNPMVGAVVVRDGRVVGEGYHRAPGEPH
                     AEVEALRAAGELATGATVYVNLEPCSHYGRTPPCAAALIEAGVARVVYACSDPNPKAA
                     GGAAWLRREGVEVVGGVLEPEAARLNEAFLKHVRTGMPFAIMKAGMSVDAQLAGASRE
                     REAITSP"
BASE COUNT          273 a          582 c          622 g          290 t
ORIGIN      
        1 ggtgatctgc ctcgccgctt gcctcctcct cggaccgggg aagttgggcg tagcgctccc
       61 cacaagcgcg cacctggtgg ttctatacac gaacgatacc cggggcgagc ttgatccttg
      121 cgaatgcagc gaactcactg gcggtcttgc gcggcgagcg accttggtgg agcagattcg
      181 cgccgagggg ttccccgtgc ttctggttga ctccggggac atcgtcgggc gtccggccaa
      241 gctccagccc atgctggatg ccatgcagcg tatcgggtac caacacgtgg ggcttggcgg
      301 tgtggatggc cttctccgcg acgcgttcgt gcgcgccgcc gccgagcgcg gcctgaccgt
      361 ccactccctc gccatgggcg agggagtggc gccgccagac gtcgtggtcg tcgaggggtt
      421 tcgcgtgggc atcgtcggtg tgggcagcga tcatctcgga ctgcccgcga gcaaggtttg
      481 cgcccgtgtc tccacggcag tggctgacct cgagtcacga gccgacctgg ttctgatcct
      541 cagccagatg ggccgaaaga tcgacagggc gatcgcttcc cggctatcgc ggggcagcac
      601 accggtggtg gtcgtgggta accgcgtgtg gcacgacctg gtcgagccgg agaacatcgg
      661 tcgggtgatc atcgtgccca cgggctacct cggacggtgg ttggggcggc tcgacgtcac
      721 gcgcctcatg ggcgccacac gcgtcgaggc gaagctgata cccgtcgcac cgacgctgaa
      781 gcagcaaccg gatgtccagc ggatcgtcaa ggcctactac gaccgggagc gagcggcagc
      841 caagcccgcc cccgtgccga ccaaggccgc gaccctgacg ggtgacccct ctgactgcat
      901 tcagtgccat cggacgcagt tcgacacctg gctgacacag aagcattccc gggctgtcgc
      961 caccctcgtg gcgaaggaca aagcgatccc cgagtgcctc atgtgccact cccaagtcta
     1021 ccggcaggaa cggcgggtgc cctcgtccga ggacattgac gatggggtgg gatgcctggt
     1081 gtgccacagc ccggcggtgg ggcacactgg ggaagagcgc cccttcagcg ttcggcgcgc
     1141 tgccggccag gcgcagtgca ttcagtgcca cacgcctgaa cgggatccag acttcgacta
     1201 tgcggcggcg cttgagcgca tccgccactg ggacgagccg gttgccatcg cgcgatgacc
     1261 atttcatgcg cagagccctc gagcttgcgg cgctcgctcg cgggttcacg agccccaacc
     1321 ccatggtcgg tgccgtggtg gttcgtgacg ggcgggtagt cggcgagggg taccatcgag
     1381 cgcctgggga gccccacgcc gaggtggagg cgctgagggc cgcgggtgag ctcgcgaccg
     1441 gagcgacggt ctatgtcaac ctcgaaccct gcagccacta cggccggacg ccgccgtgcg
     1501 cggccgcgct gatcgaggcg ggagttgccc gagtcgtcta tgcgtgctcc gatcccaatc
     1561 cgaaggctgc tggcggcgcc gcctggctcc ggcgcgaggg cgtcgaggtg gtcggtggcg
     1621 tgctcgagcc cgaagcggct cgcctgaacg aggcgttcct caagcacgtc cgcaccggca
     1681 tgccgtttgc catcatgaag gcaggtatgt cggtcgatgc acagctcgcc ggcgcgagcc
     1741 gggagcgcga ggccatcacc tcgcccg
//
LOCUS       DAOSPL010001134         1766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_67359_length_1766_cov_3.398013, whole genome shotgun sequence.
ACCESSION   DAOSPL010001134 DAOSPL010000000
VERSION     DAOSPL010001134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67359_length_1766_cov_3.398013"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLQ54_17490"
     CDS             complement(<1..510)
                     /locus_tag="PLQ54_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95112.1"
                     /translation="MSGRRALVLLLASLTCLWLAGCSGTGSSVIDSLFGGDSNAVTRG
                     FVAASGYEETAEQTDALILVRWDLPSTPSASVQPYGSYIVAWEVYRDTSSNFFPTPDK
                     LQAVLIGGKMNRFVDTTQATRGATITLSTDESGIVQPTTETLAADDDSEPARTVATDS
                     FEYTVVPTPH"
     gene            1137..>1766
                     /locus_tag="PLQ54_17495"
     CDS             1137..>1766
                     /locus_tag="PLQ54_17495"
                     /inference="COORDINATES: protein motif:HMM:NF037429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HOS95113.1"
                     /translation="MDFDAGRARAITIYADTRAHNGASRPMAEVVAALDAAESSLAGS
                     AERVDAFRESRRRAEEVLADPAAHAGEGLAAFVCESRGLLEVFTLPTPFHTRVAVGRR
                     LYVPPLARLLETFDSHIVAHVRSDRAEIYTAYLHRLWPAVELASDVPGRASGGGWAQA
                     RLQRHREECVKKHLKLVADEIARLVSEEEADRVLLFGPGEVLDALSRHLP"
BASE COUNT          272 a          577 c          603 g          314 t
ORIGIN      
        1 gtgtggtgtg ggcaccacgg tgtactcgaa ggagtccgtc gccacggtgc gtgctggttc
       61 ggagtcatca tcggccgcga gggtctcggt ggtcggctgc acgatacccg actcatcggt
      121 cgagagcgtg atggtggccc cgcgcgtggc ctgggtcgta tcgacgaacc ggttcatctt
      181 cccgccgatg agcacggcct gcagcttatc gggtgtgggg aagaagttgg agctggtgtc
      241 gcggtacacc tcccacgcca cgatgtacga gccgtagggc tggacactgg cgctcggggt
      301 cgatggcagg tcccaccgca cgaggatgag cgcatcggtc tgctcggcgg tctcctcata
      361 gccggaggcg gcgacgaatc cgcgggtgac tgcgttgctg tcgcctccga acaggctgtc
      421 gatcacgctg cttcccgtgc cggagcaacc cgcgagccac agacacgtca gactcgccag
      481 gagcagcacg agggctcttc tcccgctcat cgatagtcct ccccagttgg ataacgcagg
      541 tgtcttcgca ccccgactcg cggcctcctt cttgcccgcg atcgaaaaaa agcgatccca
      601 tggtccgcgc acgtcggggt gcgccggacc ggccgcactc ccaacggccc taacccctcg
      661 gcggcgcagg ggttcaagcc gtcacctctg tagacgaatc caggggcgac tctgactcat
      721 attatacccg cggcaatgcc gggcacgttg tgcttcgacg tgtgcattcg gcgggcctcc
      781 attgcgcgca tcggcgactt ctcttgggcc tgaggccgcc tctagacgcg gcattgagcc
      841 gaaggggctc ctgaccccat gcgcgcccac ctcgccaggg tcaggggcat tgggtgcgtg
      901 atcctgcacc aacccgaggc ggcaggcgcc gaagaggacc tcagtgcccg cagggggaag
      961 gagtctccac gttggggcta cttggcttga gaaggacggt gatccctggc cagaccgaac
     1021 cacgttgcgc gggttccgca gctccatctt gaccaccagg gggtgtcccg gcagtggcag
     1081 gtaccgagct agcccgaccg aagcgttacg tcgttcagga tgacatcgtg agcctcatgg
     1141 attttgacgc ggggcgtgcc cgggccatca caatctacgc cgacacacgg gcccacaacg
     1201 gcgccagccg gcccatggcg gaggtggtgg cggctctgga cgccgctgag tcatcgctgg
     1261 cggggtctgc cgagcgggtt gacgcgttcc gcgaaagccg gcggcgtgcc gaggaggtcc
     1321 ttgcggatcc ggccgctcat gccggagagg gcctcgccgc cttcgtgtgc gagtcgcgcg
     1381 gcctgctcga ggtgttcaca ctgcccaccc cgttccacac gcgcgtggct gtggggcgcc
     1441 ggttgtatgt cccgcctctg gctcgcctgc tggagacctt cgactcccac atcgttgccc
     1501 atgtgcgctc ggaccgggcc gagatctaca cggcctacct ccatcgcctg tggccggcgg
     1561 tggagctcgc cagcgatgtg ccgggccggg cctcgggagg tggttgggcc caggcgcgcc
     1621 tgcagcggca tcgcgaggag tgtgtgaaga agcacctcaa actcgttgcc gacgagatcg
     1681 cgagactcgt ctccgaagag gaggcggatc gggttcttct gttcgggccc ggggaggtgc
     1741 tcgacgctct gtccagacac ctgcct
//
LOCUS       DAOSPL010001135         1765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_67421_length_1765_cov_2.876608, whole genome shotgun sequence.
ACCESSION   DAOSPL010001135 DAOSPL010000000
VERSION     DAOSPL010001135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67421_length_1765_cov_2.876608"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PLQ54_17500"
     CDS             <1..800
                     /locus_tag="PLQ54_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95114.1"
                     /translation="KTICNGRLRYWVLPGGRTAWRSPDLGDPSERHEREVQFAREALK
                     YSVVQLSGANEGVAGRSTYLLVFSFRGFDQKLYKIREVLLDRQNLAILENREYGMHGL
                     RSRTYFRDIRFDTRLVSSAFEYRPGADVLVLPEPTGPPFPVGDDTRQRLGAFVEQPPV
                     PAVPSGWSPTGCIMLHYGGLSVAQFGYYVRSRDRSAVLLRHRLGETDPFFGAFFRTAE
                     ATGRVKRLGSQFCVWRGGGYLHVLASPLPESELLAAARQFQSGLSRP"
     gene            complement(829..1062)
                     /locus_tag="PLQ54_17505"
     CDS             complement(829..1062)
                     /locus_tag="PLQ54_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95115.1"
                     /translation="MRNKTLSQPVSLPVIIVVLLMFAAMLYVLFYLTFGRQSTPANAA
                     TPQHYAAAPRDSGHTSPFRSTAVGVTSSIRTGE"
     gene            complement(1171..>1765)
                     /locus_tag="PLQ54_17510"
     CDS             complement(1171..>1765)
                     /locus_tag="PLQ54_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOS95116.1"
                     /translation="VIDVVAGVAIAYLLTRRRIPGAGVLDALSVLPLALPGLVLAFGY
                     VGCFAGWPIDPRANPTALLVVAYAVRRLPYAVRSAYAGFQQTSITLEEASMNLGASPM
                     RTLRRITLPLVTANLVAGGILTFAFAMLEVSDSLILAMKEQYYPITKAILSLLQRIVD
                     GPYIASAMGVVGMVLLAVSLIVAGRFLGRRMGELFRA"
BASE COUNT          287 a          544 c          611 g          323 t
ORIGIN      
        1 tcaagacaat ctgcaacgga cgcctccgct actgggttct gcctggtggc aggacggcat
       61 ggcggtcgcc ggatctgggt gacccctcgg agcgtcatga gcgtgaggtc cagttcgcgc
      121 gggaagcgct gaagtatagt gtggtccagc tctcgggggc gaacgagggc gttgccggac
      181 gttcgaccta cctcctggtc ttcagcttcc gtggcttcga ccagaagctg tacaagatcc
      241 gggaggtgct gctggaccgc cagaacctgg ccatcctcga gaaccgtgag tatggcatgc
      301 acggtcttcg gagccgaacc tacttccgcg acatccgttt cgacacccgt ctggtctcgt
      361 cggccttcga gtaccgcccc ggggccgatg tgctggtgct tccggagccc acgggccccc
      421 cattcccggt gggtgatgat acccgccagc ggctgggtgc cttcgtcgag caaccgccgg
      481 tgcctgccgt gccatcgggg tggtctccaa cgggctgcat tatgctgcac tatggggggc
      541 tcagcgtggc ccagttcggc tactacgttc gatcccggga ccggtcggcc gtgctgctac
      601 gccaccggtt gggcgagact gatccattct tcggagcgtt cttccgcacc gccgaggcga
      661 ccgggcgggt caagcgcctt gggagtcagt tctgcgtgtg gcgcggcggg ggctacctcc
      721 atgtgctggc ttcaccgctg cccgagagcg agctgctggc ggcggcgcgg cagttccaga
      781 gcggcctttc gcgtccgtag gccgcgtcac ggagcagcgg ggctgacctc attcgccggt
      841 gcggatgctg ctggttactc cgaccgcggt cgaccggaat ggcgacgtgt gcccggagtc
      901 ccgcggtgca gcagcatagt gctggggggt cgcagcgttc gccggcgtgc tctgtctacc
      961 gaatgtgagg tagaagagca cgtacagcat cgcggcgaac atgagcaaga ccacgatgat
     1021 gacgggaagg ctcaccggtt gtgagagcgt cttgttcctc attgtcgttg ggtggcaccg
     1081 gcatcgctga ttcgtgcggt cgtgacgcta tttcagtttt cggcgaatcg ggccaaaatc
     1141 ctccgtcgac agggcccggg aggccgttga tcatgcacgg aagagctctc ccatgcgcct
     1201 gcccaggaac ctcccggcga cgatcaggct gacggccagg agcaccatcc ctaccacacc
     1261 cattgcgctg gcgatgtacg gcccgtcgac gatccgctgt agcagggaca ggattgcctt
     1321 ggtgatcggg taatactgct ccttcatggc gaggatgagg ctgtcgctga cttcgagcat
     1381 cgcgaaggcg aatgtcagga ttcctcccgc aacgaggttg gcggtcacca gcggtagcgt
     1441 gatgcggcgc aaggtgcgca tgggcgaggc gccgaggttc atcgacgctt cctcgagtgt
     1501 gatgctcgtc tgttggaacc cggcgtaggc cgatcgcact gcgtagggca gacgccggac
     1561 cgcataggcc accaccaaca gcgcggtggg gttagccctc gggtcgatcg gccagccggc
     1621 gaaacatccg acgtagccga atgcgagcac gaggccgggc agcgcgagcg ggagcaccga
     1681 gagggcgtcg aggactcccg cgcccggaat gcggcggcgt gtgagcaggt aggcgatggc
     1741 gacgcccgcc accacgtcga tcacc
//
LOCUS       DAOSPL010001136         1765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_67430_length_1765_cov_2.529825, whole genome shotgun sequence.
ACCESSION   DAOSPL010001136 DAOSPL010000000
VERSION     DAOSPL010001136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67430_length_1765_cov_2.529825"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLQ54_17515"
     CDS             <1..212
                     /locus_tag="PLQ54_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95117.1"
                     /translation="AVKRLIDASLATGVGHFASVHEAAQAWYRPGRQIRPDPERSATY
                     EEIYDRYLDLARRVYGRERGGERAT"
     gene            209..1039
                     /locus_tag="PLQ54_17520"
     CDS             209..1039
                     /locus_tag="PLQ54_17520"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOS95118.1"
                     /translation="MRNSMLFRDQTSPIIGEHARRGSLLILPIGTTEEHGPHLPVDTD
                     ARIAEAYGARLAEAVATDLPVLVLDTIRYGYSMQIMRRWPGTIVVRSRVFMDLVFDVV
                     RSTLDMGFRKLVILDCHGHHSGPLNTVSREICDACHKSIAIISPAVLSRDEFNQVRKS
                     GQGGAIHGGEWETSLLLHISPEVVDMSLAPSGDAMRYHSDFVAGDGFMGRQRVTWSTW
                     YLQESQSGVYGDPTPATAETGRVIMDAAVANGSRFLREFWRQPDAVCRCPQDGPNPGP
                     "
     gene            1087..>1765
                     /locus_tag="PLQ54_17525"
     CDS             1087..>1765
                     /locus_tag="PLQ54_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95119.1"
                     /translation="MAAVARKSGECSVVCGCRIRHRPTWWPTLALTALLAATAPRARA
                     TVELAGDAIIASGPGYRFELSARNGAIEAVRKGPGSPSRLRSGEHGLWLIRFADGTTL
                     DSTGFSKDDAAHQFTAHIPADDGSLRLVYECADAGVTVDLAPDDRGLAITAKVRAGAR
                     PVLDLQVPARLRFEPEALRRLIVPSDGNQGVGLALNSRFFSPQPQDQPVSWAPTPAGP
                     AAYESLLG"
BASE COUNT          264 a          649 c          585 g          267 t
ORIGIN      
        1 gtgccgtgaa gcggctgatc gacgcctcgc tcgccaccgg cgtcggccac ttcgccagcg
       61 tccacgaggc tgcccaggcc tggtaccggc ccgggcgcca gatacggccc gatccggagc
      121 gatcggccac ctacgaggag atctacgacc gctatctcga cctggcccgc cgggtctacg
      181 ggcgcgagcg cggtggggag cgggccacat gagaaactcc atgctcttcc gtgaccaaac
      241 gtcaccgatc atcggcgagc acgccaggcg cggctcgctg ctcatcctgc ccatcggcac
      301 caccgaggag cacggccccc atctgcccgt cgatacggac gcgcgcatcg ccgaggccta
      361 cggggcgcgc ctggccgagg ccgtggcaac cgatctgccg gtgctcgtcc tggacaccat
      421 ccgctatggc tactcgatgc agatcatgcg gcggtggccc ggcaccatcg ttgtccgctc
      481 ccgcgtgttc atggacctgg tcttcgatgt cgtccgctcg acgctggaca tgggattccg
      541 caaactggtc atcctcgact gccatgggca tcactcgggg ccgctgaaca cggtctcgcg
      601 cgagatctgc gatgcgtgcc acaagtccat cgcaatcatc agcccggccg tgctctcgcg
      661 ggacgagttc aaccaggtcc gcaagtccgg gcagggcggg gcaatccatg ggggcgagtg
      721 ggagacctcg ctgctgctgc acatcagccc tgaggtggtc gacatgtcgc tggcccccag
      781 cggtgatgcc atgcgctacc actccgactt cgtcgccggc gacgggttca tgggccggca
      841 gcgcgtcacg tggtcgacgt ggtacctgca ggagagccag tcgggggtct acggcgaccc
      901 cactccggca acggccgaga cggggcgcgt catcatggac gccgccgtcg ctaacggcag
      961 ccgattccta cgggagttct ggcgccagcc cgacgcggtc tgccgatgcc cacaagacgg
     1021 cccgaatcca ggaccgtgag acccgggccc ggcccggcta agggtaacca acgcgggcgg
     1081 cctcgcatgg ccgcggtcgc ccggaagtcg ggggaatgct ccgtggtgtg cggttgtcgg
     1141 atccgccacc ggccaacgtg gtggcccacg ctcgccctga cggcgcttct cgctgccacg
     1201 gcgccccggg ccagggccac cgtcgagctc gccggcgacg ccatcatcgc ctccggcccg
     1261 ggctacagat tcgagctcag cgcccggaac ggcgccatcg aggccgtgcg gaaggggccc
     1321 ggctcaccca gccggttgcg ctcgggcgag catggactct ggctcatccg cttcgccgat
     1381 ggaacgaccc tcgactccac gggcttctcg aaggacgatg cggcccatca gttcaccgcc
     1441 cacataccgg ccgacgatgg ttccctgcgc ctggtgtacg agtgcgccga cgcgggcgtg
     1501 acggtggatc tcgccccaga cgaccgcggt ctcgccatca cggccaaggt ccgggccgga
     1561 gcccgccccg ttcttgacct ccaggtgccg gcccggctgc gcttcgagcc ggaggccctc
     1621 aggcgactga tcgtaccctc ggacggcaac cagggcgtgg gcctggcgct caactcccgg
     1681 ttcttcagcc cccagcccca ggaccagccg gtctcgtggg cgcccactcc agccggcccg
     1741 gccgcctacg agtcgctctt gggtg
//
LOCUS       DAOSPL010001137         1764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_67471_length_1764_cov_2.530135, whole genome shotgun sequence.
ACCESSION   DAOSPL010001137 DAOSPL010000000
VERSION     DAOSPL010001137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67471_length_1764_cov_2.530135"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1764
                     /gene="pckA"
                     /locus_tag="PLQ54_17530"
     CDS             163..1764
                     /gene="pckA"
                     /locus_tag="PLQ54_17530"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOS95120.1"
                     /translation="MSPTEKDQWRAKLQEHGLRNFGEVHWNATTAELYEHAVRRREGI
                     ISHQGPLSVRTGHHTGRSPRDKFIVREPSSEAHIWWDDQVNRPFSSVQFDQLHQRLLA
                     YMQGQDVFVQDCYVCNHPRYRIPVRVITETAWHSLFTRTMFIHIANRDEVRDHVPQVT
                     IIHAPHFHAMSELDGTQSEAFIIINFGKNLAIIGGTQYGGEIKKCVFTILNYLLPNEH
                     VLSMHCSANVGSDGDVAIFFGLSGTGKTTLSADPQRRLIGDDEHGWAEDGIFNFEGGC
                     YAKVIRLSREAEPDIYACTEKFGTILENVRVDRITRRVDLNDDTLTENTRAAYPIDSI
                     PNSVPEGRGGHPKNIIMLTCDAFGVLPPVAKLTYEQAMYHFLSGYTARVAGTERGVTE
                     PSATFSACFGAPFLARPPAVYAQMLGENIRRHHVDCWLVNTGWTGGGYGVGHRIDIKA
                     TRRIIQAILDGTLSKAEYRADPTFRAEVPTSCPGVPSEILVPRDRWADHEAYDATAAS
                     LVQMFRQNFAKFTDDISAEIARHGL"
BASE COUNT          325 a          574 c          548 g          317 t
ORIGIN      
        1 cgccgggggg cccagcgcgc ccggccccgg cggcgtcatg ggcggaccgc cccccgggct
       61 ggtcgcacgc tgaaggatac cgcgtcaccc agatgatccc atgctttgag atccgcggcc
      121 gggtcgctat aatcgcccat tgcgtgagga gggtggctgc ggatgagccc aaccgagaag
      181 gaccagtgga gagcgaagct gcaggagcac ggcctccgga acttcgggga ggtgcactgg
      241 aatgccacga cggctgagct ctacgagcat gcggtacggc gccgggaagg catcatcagt
      301 caccaggggc ctctgagcgt ccgcacgggc caccacacgg ggcgctcgcc gcgcgacaag
      361 ttcatcgtgc gtgaaccatc gagtgaggca catatttggt gggacgacca ggtcaaccgg
      421 cccttctcca gcgtccagtt cgaccagctc caccagcgct tgctggccta tatgcagggc
      481 caggacgtgt tcgtccagga ctgctacgtg tgtaatcatc cccgctaccg cattccggtg
      541 cgggtcatca ccgagacggc gtggcacagt ctgttcacgc ggactatgtt catccacatc
      601 gccaaccgtg acgaggtccg ggatcatgta ccccaggtta cgatcatcca cgcgccgcac
      661 ttccatgcca tgtccgagct cgatggcaca cagtccgagg cattcattat cattaacttt
      721 ggcaagaacc tggccatcat cgggggcact cagtacggcg gggagattaa aaagtgtgtg
      781 ttcaccattt tgaactatct tttgcccaat gagcatgtgc tctccatgca ctgctcggcc
      841 aacgttgggt ccgatggcga tgtggccatc ttcttcggct tgtccggcac gggcaagacg
      901 accctctcgg ccgacccgca gcgccggctg atcggtgacg atgaacacgg ctgggccgaa
      961 gacggcatct tcaacttcga gggcgggtgc tacgccaagg tgatccggtt gtcccgagag
     1021 gcggagcccg acatctacgc gtgcaccgag aagttcggca cgatcctcga gaacgtgcgc
     1081 gtcgaccgca tcacgcgccg ggttgacctc aacgatgaca cgctgacaga gaacacccgg
     1141 gcggcctacc ccattgacag catccccaat tccgttcctg aggggcgcgg cggccatccg
     1201 aagaacatca ttatgctcac ctgtgatgcg ttcggcgtct tgcctcccgt ggccaagctc
     1261 acgtacgagc aggccatgta ccacttcctc tcggggtaca ccgctcgcgt cgcgggaacc
     1321 gagcgcggcg tcaccgagcc ctcggcaacg ttcagtgcct gcttcggcgc acccttcctg
     1381 gctcgccccc ccgcggtcta cgctcagatg cttggtgaga acattcgcag gcaccatgtg
     1441 gactgctggc tcgtgaacac cggctggacg ggcggtggct acggagtcgg ccaccgcatc
     1501 gatatcaagg cgactcgccg catcatccag gcgatcctcg atggcacgtt gtcgaaggcc
     1561 gagtaccgtg cggatcccac gttccgggcc gaggttccga cgtcgtgtcc gggggtgccc
     1621 agtgagatcc tcgtgccgcg cgaccggtgg gccgaccacg aggcgtatga tgccacggcg
     1681 gcgtcactcg tccagatgtt ccggcagaac ttcgccaagt tcacggacga catctcggcg
     1741 gagatcgcgc gtcacgggct ctag
//
LOCUS       DAOSPL010001138         1760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_67667_length_1760_cov_2.622874, whole genome shotgun sequence.
ACCESSION   DAOSPL010001138 DAOSPL010000000
VERSION     DAOSPL010001138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67667_length_1760_cov_2.622874"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="PLQ54_17535"
     CDS             <1..1129
                     /locus_tag="PLQ54_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95121.1"
                     /translation="GRPYAAVTEAWLSLANGATRRSLLDHDLLHLGTAQRQEAGHVTS
                     WLDLWTHAGDHGEWSLRARQWPWYLSYMVEEAAAKSCGVVGDVPPIDRSVTLDVCTPA
                     LGDLLSLLSPDAGMELLCDGSLVGMRLAVGAKDAPVDDVLASIAFSLAAVWRHIGDCL
                     YLGDAKAAVDAAAATPESRRSGNTAVLLPVGPNSDAALAAAAGPASFPLLEIRWTAGE
                     TTGRTLVISYDPSRGLVRAIPPMPGPEGERSLHCAVEAVAGLAESQGMPLTADALQRL
                     IDRVGTGSPVDGNALIGLGADVGLSLVGARGTLSEVSAVPDACIVHLAFGHYACVSGL
                     GRSFSLVHGATGRRGRFPTAFVEQGFSGHLFVDSAVAERLK"
     gene            1161..>1760
                     /locus_tag="PLQ54_17540"
     CDS             1161..>1760
                     /locus_tag="PLQ54_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95122.1"
                     /translation="MAVRCSMLTGSGVLSVLAAACLAVTSKPDPGPARASLRIVYTID
                     TGGQISTCGCSGGQHGGLARRASLLKALALDGVPLVVLDGGSVVTSASQSPYFAEAYA
                     AMRYSLVIPSQADVTARDGDGNGLSEALNALGVAVLARPIADAADPLPSAAIDVAQGW
                     RLLALAAPEGALPLPALAARARQAVAGVDRARTAILLATR"
BASE COUNT          237 a          585 c          624 g          314 t
ORIGIN      
        1 aggaaggccc tacgctgcgg tcaccgaggc atggttgtca ttggcgaacg gggcaacccg
       61 gcggtcgctc ttggaccatg atctactgca cctcggcact gctcagcggc aggaggctgg
      121 gcacgtcacc tcatggctcg acctgtggac ccatgcgggt gaccatggcg aatggagcct
      181 ccgtgcccgc cagtggccct ggtacctctc gtatatggta gaggaggccg cggccaagtc
      241 gtgcggcgtc gtcggtgacg tgccgcccat cgatcggagt gtcacgctcg acgtgtgcac
      301 gccggcgctc ggggacctcc tgagcctgct gtcgcccgat gcggggatgg agctgctgtg
      361 cgacggctcg ctcgtgggca tgcgcctagc tgtcggagcc aaggacgcac ccgtagacga
      421 cgtcttggca tcgatcgcgt tctcgctcgc cgcagtatgg cgccacatcg gcgactgcct
      481 gtacctcggg gatgccaaag cggctgtcga tgcggccgcc gccaccccgg agagcaggcg
      541 gtcaggcaac acagcggtgc tgttgcctgt cgggccgaac tctgatgctg cactcgccgc
      601 tgcggcgggt ccggcctcat tccctctcct ggagatccga tggactgccg gggagaccac
      661 cggccggacg ttggtcatct cttacgaccc ttcccgtggg ctggtccgcg cgatacctcc
      721 catgcccggc ccggaaggtg agcgctctct gcactgcgcg gtcgaggccg tcgctgggct
      781 cgccgagtcg caggggatgc ccctaacggc ggatgcgctc cagcgactga tcgaccgcgt
      841 gggcacgggc agtcccgtcg acggcaacgc cctgatcggc ctgggggctg acgttgggct
      901 gagcctcgtt ggggcgcgag ggaccctctc tgaggtaagt gccgtcccgg acgcatgcat
      961 cgtccacctc gcgtttgggc actacgcctg tgtcagcggg ctgggccgat cgttctcgtt
     1021 ggtccacggc gcgacgggac gccgtggacg cttcccaacc gctttcgtcg agcagggctt
     1081 ctctggtcac ctgttcgtgg attcggctgt ggccgaacgt ctgaagtgag gaggcccgtc
     1141 cgggccgtcg atgggggaac gtggcagtgc gctgctccat gctgacgggc tcaggcgtcc
     1201 tctccgtgct ggcagccgcg tgcctcgcgg tgacctcgaa gcctgacccg ggcccagccc
     1261 gggcgagcct tcggatcgtc tacacgatcg acacgggcgg ccagatcagc acgtgtggct
     1321 gctcgggcgg acagcacggc gggctggcgc ggcgggcgag tctgctgaag gctctggccc
     1381 tcgatggcgt gccgctggtg gtgctcgacg ggggttctgt cgtcacgagt gcatcccaga
     1441 gcccctactt cgctgaggcg tacgctgcca tgcggtacag ccttgtcatc ccaagtcagg
     1501 ccgatgtgac ggcgagggac ggcgacggca atggcctctc cgaagccctg aatgcgttgg
     1561 gcgtggcggt tctggctagg ccaatagccg atgcggctga cccgttgccc tcggctgcta
     1621 tcgatgtagc ccagggatgg cgcctgctcg ccctggcggc tcccgagggc gcgttgccac
     1681 tgccggccct tgccgcccgg gcgcggcaag ccgtggcggg cgtcgacaga gccaggacgg
     1741 ccatcctcct cgccactcgg
//
LOCUS       DAOSPL010001139         1758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_67772_length_1758_cov_3.177921, whole genome shotgun sequence.
ACCESSION   DAOSPL010001139 DAOSPL010000000
VERSION     DAOSPL010001139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67772_length_1758_cov_3.177921"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PLQ54_17545"
     CDS             complement(<1..277)
                     /locus_tag="PLQ54_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HOS95123.1"
                     /translation="MEPVVDLRSDTVTRPTPEMRRAMAEAEVGDDVAGDDPTVNRLEA
                     TAAEFIGKEAALFVPSGTMGNLCAILVATRPGDALVCGHLTHSAEYEG"
     gene            447..1517
                     /locus_tag="PLQ54_17550"
     CDS             447..1517
                     /locus_tag="PLQ54_17550"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS95124.1"
                     /translation="MADERIGFAIAGCGVIAPWHAYGITHSGLTELRAVFDVVFDRAK
                     GLAADVASKYGVPEPRVCRTLDELLEDSSVSALSVCTPSGLHAEIGIAAAGAGKHVLC
                     EKPIDITLEHIDALVRACQDAGVKLGCIFQRRTSPLWQGIRRAVREGALGKMLLGDAY
                     LKYYRSQAYYDSGDWRGTWALDGGGCLMNQGVHCIDQVQWIMGPVRSLYAQTDHLARS
                     IEVEDVACAVLRFESGAMGVLEGTTGIAHPGVDHRLEFHGETGTIRVFEESIVEWNVP
                     GVDQGEFIPRGTAGQEAATADPTAIGMEGHLQQIRDMAEAIRDDRDPMVTGPEARKAV
                     EIILAVYRSSQQGQPVSFPLEQ"
     gene            1521..>1758
                     /locus_tag="PLQ54_17555"
     CDS             1521..>1758
                     /locus_tag="PLQ54_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95125.1"
                     /translation="MPDEPIIDCHVHPDPVDLPEGLIREANRLGVTQLIVASLGRSWD
                     YEPSHEHCVRGNRDTLALVDRYGGMVLGWAYVNPR"
BASE COUNT          301 a          554 c          604 g          299 t
ORIGIN      
        1 caccctcata ctcggcgctg tgcgtaaggt gaccgcacac cagtgcgtca ccgggccgcg
       61 tcgcgaccag gatggcgcac aggttaccca tggtcccgct gggaacgaac agggcggcct
      121 ccttgccgat gaactccgcc gccgtcgcct ccagccggtt cacggtgggg tcatcgccgg
      181 cgacatcatc accgacctcg gcctcggcca tcgcccggcg catttccggc gtcggccgcg
      241 tcacggtatc gcttcgtagg tccaccactg gctccatggc cgggggctca ccatcctcaa
      301 ccttccccgt ggcggcgacc agacaagccg cgacgagcga agggactcgc gcgcagactg
      361 gcgcacatag tagagcatcg gcgcggctgt ggccacgccg cgtcgactcg cgatgaggca
      421 cctccgaaag atggggagag gcgtcgatgg cggacgaacg gatcggcttt gccattgcgg
      481 gctgcggcgt tatcgctccg tggcacgcct acgggatcac gcactcaggg ctgacggaac
      541 ttcgagccgt gttcgatgtg gtgttcgacc gagccaaggg cctcgccgcg gacgtggctt
      601 cgaagtacgg tgtgcccgag ccgcgcgtgt gccggaccct ggacgagctg ctggaggaca
      661 gctcggtctc cgcgctgagt gtttgcacgc ccagcggcct gcatgcagag atcggcatcg
      721 cggcggctgg ggctgggaag catgtgctgt gtgagaagcc catcgacatc acgctggagc
      781 acatcgacgc cctagtgcgc gcctgccagg acgccggcgt gaagcttggg tgcatcttcc
      841 agcggcgcac ctcgccgctc tggcagggga ttcgacgggc cgttcgggag ggcgcgctag
      901 gcaagatgct tctgggtgat gcatatctca aatactaccg aagccaggcc tactacgaca
      961 gcggcgactg gcgaggcacg tgggccttgg acggtggcgg atgcctgatg aaccagggcg
     1021 tgcactgcat cgaccaggtc cagtggatca tggggccagt gaggtccttg tacgcacaga
     1081 ccgaccacct ggcccgaagc atcgaggtcg aggacgtggc atgcgcggtg ctccgtttcg
     1141 agtcgggcgc catgggagtg ctggagggca cgactggcat cgcacatccg ggtgtggacc
     1201 accggctcga gtttcacggg gagacgggca cgatccgcgt attcgaggag tctatcgttg
     1261 agtggaatgt gccgggagtc gatcagggcg agttcatccc ccggggcacg gctgggcagg
     1321 aggcggcgac ggccgatccc actgcgatcg gcatggaggg tcaccttcaa cagatccgcg
     1381 acatggctga agccattcgg gacgaccgag accccatggt gaccggtccg gaggcccgca
     1441 aggcggtgga gatcattctg gcggtctacc ggtcttcgca gcaaggccaa ccggtgtcat
     1501 tcccgctgga gcagtagccg atgccggacg agcccattat cgactgccac gtacacccgg
     1561 acccggttga ccttcccgag gggctcattc gcgaggccaa ccgcttgggc gtgacacaac
     1621 tcatcgtggc ctcgctcgga cgctcgtggg actatgagcc ctctcacgaa cactgcgtgc
     1681 gaggcaatcg ggacaccctc gccttggtgg atcgctatgg gggcatggtg cttggttggg
     1741 cctacgtcaa tcctcgct
//
LOCUS       DAOSPL010001140         1755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_67932_length_1755_cov_3.714118, whole genome shotgun sequence.
ACCESSION   DAOSPL010001140 DAOSPL010000000
VERSION     DAOSPL010001140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67932_length_1755_cov_3.714118"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1636
                     /locus_tag="PLQ54_17560"
     CDS             <1..1636
                     /locus_tag="PLQ54_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOS95126.1"
                     /translation="RELNVPIGLINSSWGGTAIDPWVAPVGYVPIPALQGTLEAVTLT
                     DPRSEPYKKALGDYLTQLDAWRARADQALATETPLDPAPAYPDTIKPMNTVGQPTALY
                     NGMIAPLVPYGIRGAIWYQGESNHGEGMLYAEKMKALIGGWREVWQQGDFPFYYVQIA
                     PWNYGDEDPGILPVFWEAQAAAQSIPNTGMAVINDIADYNDIHPKNKQDVGKRLALLA
                     LNKTYGRTNVVCSGPTFRELATEGDTLRVRFDNVGGGLVSRDGQPLTWFEIIGPESDY
                     TKADAVIDGDSVLLTAPGVEKPCAVRFAWHKSAEPNLSNKEGLPAGAFRAGEVPVIDY
                     LTVKVDEAKDYQLAYDIDLGKLGADVTYDVNNAGTLAGAFDRVAYFLDLQKPGEGVKY
                     VYVSMDAFTDDITKIGIPTIASGARFQCPVANMTIISNVSGLATGVGLSGGCIEFWSN
                     NYGPPNAANVPNASNDLWDFGDQMDGGTVDGYGCMQVGNSEAQQTVFAINNWKAGAGA
                     DIGIGNSEGQTRDWTFTGNARGYSLKRLRVLVRPKY"
BASE COUNT          330 a          559 c          582 g          284 t
ORIGIN      
        1 ccgcgagctc aatgtgccca tcggcctgat caactcctcg tggggcggca cggccatcga
       61 cccgtgggtg gcccctgtgg gctacgttcc catccccgcg ctgcagggca cgctcgaggc
      121 cgtgacgctc accgacccgc ggtccgagcc ctacaagaag gccctgggcg actacctgac
      181 ccagctcgac gcctggcgcg cccgggcgga ccaagcattg gcaaccgaga cgccgctcga
      241 cccggcgccc gcctacccgg acacgatcaa gcccatgaac acggtgggcc agcccaccgc
      301 gctgtacaac ggcatgatcg cgccgcttgt gccgtatggc atacggggtg ccatctggta
      361 tcagggcgag tcgaaccatg gcgagggcat gctctacgcg gagaagatga aggccctcat
      421 cgggggctgg cgcgaggtat ggcagcaggg ggacttcccc ttctactacg tgcagatcgc
      481 gccgtggaac tacggcgacg aggacccggg tatcctgccg gtcttctggg aggcccaggc
      541 cgccgcccag agcatcccga acaccggcat ggccgtcatc aacgacatcg cggactacaa
      601 cgacatccac ccgaagaaca agcaggacgt gggcaagcgc ctggcgctgc tggctctcaa
      661 caagacctac ggccggacca atgtggtgtg ctccgggccc acgttccgcg agctcgcaac
      721 cgagggtgac actctccgcg tgcgtttcga caacgtgggc ggcggtctgg tctctcgcga
      781 cggccagccg ctaacttggt tcgagattat cgggccggag agcgactaca ccaaggccga
      841 tgcggtcatc gatggcgact cggtgcttct caccgccccc ggcgtcgaga agccgtgcgc
      901 tgtacgtttc gcctggcaca agagcgccga gccgaacctg tcgaacaagg aaggcctgcc
      961 cgcgggcgcc ttccgggcgg gagaggtgcc ggtcatcgac tacctgaccg tgaaggttga
     1021 tgaggccaag gactaccagc tcgcctacga tatcgatctc ggcaagctgg gcgctgatgt
     1081 gacctacgat gtgaacaacg ccggcacgct cgctggcgcc ttcgaccgcg tggcctactt
     1141 cctcgacctc cagaagccgg gcgagggtgt gaaatacgtc tacgtgtcga tggacgcatt
     1201 caccgatgac atcacgaaga tcggtatccc cacgatagca tcgggtgccc ggttccagtg
     1261 ccccgtggca aacatgacca tcatctcgaa cgtgagcggc ttggcgacag gagtcgggct
     1321 gagcggcggc tgtatcgagt tctggtcgaa caactatggt ccgccgaacg ccgcgaacgt
     1381 ccccaacgcc tccaatgacc tctgggactt cggcgatcag atggatggcg gcacggtgga
     1441 tggctacggc tgcatgcagg tgggcaatag cgaggcccag cagaccgtgt tcgccatcaa
     1501 caactggaag gccggtgcgg gggccgacat cggcattggc aacagcgagg gccagacgcg
     1561 cgattggacg ttcaccggca atgcccgcgg atactcgctg aagcgcctgc gggtgctggt
     1621 gcgcccgaag tactaggaag ggctctgtgg ccggccgtcg aacccgctga gcagctactt
     1681 gctgaggccg ttactgaggg tgaggagtcg cgaaggaggt cgatgtgctg gtgagacggg
     1741 cgctgtgttg tgggt
//
LOCUS       DAOSPL010001141         1754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_67987_length_1754_cov_2.743967, whole genome shotgun sequence.
ACCESSION   DAOSPL010001141 DAOSPL010000000
VERSION     DAOSPL010001141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67987_length_1754_cov_2.743967"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..593)
                     /locus_tag="PLQ54_17565"
     CDS             complement(141..593)
                     /locus_tag="PLQ54_17565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS95127.1"
                     /translation="MASSGGSSGWRPAPLGGGYSAARDELEILVADDVPDVRQVIADT
                     LRMEGHRVTTASNGDSAIRLLREQHFDLVFTDLNMPPGISGFALIAALREVNAEVPIV
                     VISGTFVDRVNNEIAQSGTVLGTLRKPFQLLDLLDFVEQVRLRKLGAD"
     gene            925..>1754
                     /locus_tag="PLQ54_17570"
     CDS             925..>1754
                     /locus_tag="PLQ54_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95128.1"
                     /translation="MRRHLRAAPLVVLLALFACAPGSAPAQGGATITGRVTLGDAGPG
                     LPMIALVLEDPASTLADTLPTVPDRERATGKHVAMSDPRDGRFQMTGVPAGTWRLACY
                     AGAERVGEPLELVVTDGQPCNVDIALTPVAITRGRLTAEADGLPLAGVTVALVRPPGY
                     AHVAQVGSQVDLQDYRVAVTQAGGDFVLVNVLPGEWGAVVVDGTEVPRALGVITVSGE
                     PDELLELRAPHAPALGLRILDANGTPVANAEIRAWTRRDPGYAFWNEHGQPLTTTADG
                     "
BASE COUNT          266 a          593 c          612 g          283 t
ORIGIN      
        1 gctatcggtc agcggccgag gatcgcgcca tccagttccc gatgctctaa gccgtctcat
       61 cgctgggccc ggcgcccgcc gggcccagct tcgccttgtg tcgcgcggga gcacacgccg
      121 gggccgatcc acaatcgacc ctaatccgcg cccagcttgc gcagccggac ctgttcgacg
      181 aagtcgagga gatccaggag ctggaagggc ttccgtaatg tgccgaggac cgtgccactc
      241 tgagcgatct cgttgttcac ccggtcgacg aatgtgcccg agatgaccac gatgggcacc
      301 tcggcgttga cctccctgag agcggcgatg agggcgaagc cgctgattcc cggaggcatg
      361 ttgaggtcgg tgaagaccag gtcgaagtgc tgttcgcgca gcagtcggat ggccgagtcg
      421 ccgttggagg cggtggtgac gcggtggccc tccatgcgga gtgtatcagc aatcacttgc
      481 cgcacatcgg gtacatcgtc tgccaccaga atctcgagct cgtcacgcgc ggcggagtac
      541 ccgcccccga ggggcgcggg cctccagccc gacgaaccgc cgcttgatgc cacgaacgct
      601 cagctcccac tccagcgcca ccggccggcc ccttctcctg cggccgagtg ggcgccgccc
      661 cggctccggt ccggtcggcg cgcgtactgg ggcttgtgga ttcttccgcg ttgcggcctc
      721 gacgccctgt cgtcccgtga caacaacatg taaggccccc ggacaacgcc gcgcaacctg
      781 tgaacggcgc cgaccatcat acactgttgg tgacgcgatg gcgccggtcg tgcgggcgga
      841 gtatctcgcc acgcagggca atctcgaccc gggtgacgaa ctacagctta ccgagttcgc
      901 cagccgactg ggaggcgcgg tgggatgcgg agacatctgc gagctgcccc cttggtggta
      961 cttctggcgc tgtttgcgtg cgccccggga tctgcgcccg ctcagggcgg cgccacgatc
     1021 acggggcggg ttacgctggg cgatgctgga ccggggctgc ccatgatcgc tctcgtgctc
     1081 gaggatcccg cctcgacgct cgcggacacg ctgcctacgg tgcctgatcg ggaacgcgcc
     1141 accgggaagc atgtggccat gtccgacccg agggatggcc gcttccagat gaccggcgta
     1201 cctgcgggga catggcggct ggcctgctac gcgggcgccg aacgtgtggg cgagcccttg
     1261 gagttggtgg tcactgatgg ccagccctgc aacgtcgaca tcgcgctgac accggtggcc
     1321 atcacccgcg gtcgactcac ggcggaggcc gacggtctgc ccctggctgg ggtgacggtg
     1381 gcgctggtcc gcccccccgg ctacgcccat gtcgcgcagg tgggcagcca ggtcgatctc
     1441 caggactacc gcgttgccgt cacgcaggcc ggcggggatt tcgtcctggt gaacgtcctg
     1501 cccggcgaat ggggtgccgt cgtcgtggac ggcacggagg taccgcgtgc cctgggcgtc
     1561 atcacagtca gtggggagcc ggacgaactg ctggagctgc gcgccccgca cgcgcccgcc
     1621 ctggggcttc ggatcctgga cgccaacggc accccggtgg caaacgccga gatcagggcg
     1681 tggacacggc gcgatccggg ctatgcgttc tggaacgagc atgggcagcc gctgacaacg
     1741 accgcagacg ggag
//
LOCUS       DAOSPL010001142         1753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_68046_length_1753_cov_2.669611, whole genome shotgun sequence.
ACCESSION   DAOSPL010001142 DAOSPL010000000
VERSION     DAOSPL010001142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68046_length_1753_cov_2.669611"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..1052
                     /locus_tag="PLQ54_17575"
     CDS             255..1052
                     /locus_tag="PLQ54_17575"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOS95129.1"
                     /translation="MRIAIIGLAQSGKSTVFTALTGQHGWSADGLLESELTPVAEARR
                     PAPNGQTVEIIDTPGLCRGSSLGRGAGGAFLREIADVDLLLVVVRLFGRGLIAMDHPE
                     RSVDGFRDARIVDEELAMSDASAIREWLGSDSGGGAEKGAALAAILNAIEHGQMARTM
                     GLAADELRDLTEIDLLTAKPLILIANVDEEDLYSETGLPFPDELRQKAEIAGTPLLVC
                     CARLEADSACVADTGTEASDGLVDELTGGQDLLTRVYSVCEHSGLFA"
     gene            1210..1698
                     /gene="rpsF"
                     /locus_tag="PLQ54_17580"
     CDS             1210..1698
                     /gene="rpsF"
                     /locus_tag="PLQ54_17580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOS95130.1"
                     /translation="MKRYETLYIVPSALTFEEAREVALKYRAFAEERGAEIERLEDMG
                     QRKLAYEINGHFDGRYVLMVYRAPSSVPAELGHQMRLDEKVIRHITIVETKAMKRAAQ
                     AKARAEAEAATAETSPPSGAEPGEDTAEPQESAEEAAAGAAWDDEAADDEDLDEGDDA
                     EQ"
BASE COUNT          310 a          542 c          648 g          253 t
ORIGIN      
        1 gctagtgagc cctccgcccc acacgcggct cccgcaacgg ccgacccacc accgtgccgt
       61 ggaggcgttc cggtgatggc gcacgccact cccgggggag gcgggcatga cgccggcggg
      121 gtcaggctgt ccgaacacgt caccggcgag caccggagcg gctgggccga actgctataa
      181 tgccgacgac gggcagcgtc tgaggggcag agctcagggg ccgcgccagc cggataggtg
      241 gaagatcccg acgtatgcgc atcgccatca tcggactggc ccagtcgggc aagtcaaccg
      301 tgttcaccgc actgaccggc cagcacggat ggtcggccga tgggctactg gagtccgagt
      361 tgacgcccgt ggctgaagcg cgacgtcccg cgcccaacgg gcagacggtg gagatcatcg
      421 acaccccggg gctgtgccgg gggtcgtcgc tgggtcgagg cgcaggtggc gcattcctcc
      481 gcgagatcgc ggacgtggac ctccttctgg tggtcgttcg gctgttcggg cgtggactga
      541 ttgccatgga ccacccggag cgcagcgtcg acgggttccg ggacgcgcgt atcgtggatg
      601 aggagctggc gatgagcgat gcgtcggcca tccgcgaatg gctgggcagc gattccggag
      661 gcggggcgga gaagggtgcc gccctggccg cgattctcaa cgccatcgag cacgggcaga
      721 tggcccgcac catggggctg gcggccgacg aactccggga tctgaccgag atcgacctgc
      781 tcaccgcgaa gccgctgatc ctcatcgcca atgtggatga ggaggacctg tacagcgaga
      841 ccggcctgcc gtttcccgac gaactgcgcc agaaggccga gatcgcgggc acacccctgc
      901 tggtgtgctg cgccaggctc gaggcggatt cggcctgtgt cgccgacacc ggtaccgagg
      961 cctcggatgg gctggtggac gagctcaccg gcggtcagga cctgttgaca agggtctata
     1021 gcgtgtgcga acatagcggg ctgtttgcct gatccacgcc accgcgcgca ggccttcccg
     1081 aggcgcggtc cacagggcgt ggcgttcctt ctgcccgggg gttacgggag ccgccgggcc
     1141 gcctcggttc gccagccaag atacgcggct ggctccggga cccagtccag agagggaggt
     1201 cctatcccgg tgaaacggta tgagaccctg tacattgtgc cctcggcgct caccttcgag
     1261 gaagcgcgcg aggtcgcact gaagtaccgg gcattcgcgg aggaacgagg agccgagatc
     1321 gaacgcctgg aggacatggg acagcggaag ctggcctacg agatcaatgg gcacttcgat
     1381 ggccgctatg tcctgatggt gtaccgggct ccatcgtcgg ttccggcgga actcggacac
     1441 cagatgcggc tggatgagaa ggtcatccgg catattacca tcgtggagac caaagccatg
     1501 aagcgggccg cccaggccaa ggcccgggcc gaggcggagg ccgcgacggc ggagacgtct
     1561 cccccgagtg gggcggagcc gggtgaggac accgccgaac cccaggagtc ggccgaggag
     1621 gccgcggcgg gcgcggcgtg ggacgatgaa gcggccgacg atgaagacct tgacgagggc
     1681 gacgacgccg agcagtagtt cggaatcgta acgcatggac cgaggagcca ttcacgggga
     1741 gtgaggcgct atg
//
LOCUS       DAOSPL010001143         1750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_68259_length_1750_cov_1.982301, whole genome shotgun sequence.
ACCESSION   DAOSPL010001143 DAOSPL010000000
VERSION     DAOSPL010001143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68259_length_1750_cov_1.982301"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PLQ54_17585"
     CDS             <1..741
                     /locus_tag="PLQ54_17585"
                     /inference="COORDINATES: protein motif:HMM:NF017121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF726 domain-containing protein"
                     /protein_id="HOS95131.1"
                     /translation="EKLKDGPGIPVVVCSGFLTEKVKGWGDWERIVTERYPDSPVFRV
                     HWGAEELQDLYAVISGNAGKAALAKFAGKAVLKASKQAAKKAGPIGGALVVVDLVKNP
                     WWRARERANKTGAIVAELIARTSVDEVVLVGHSLGARVMLYAADALSLKSDAPRVRDV
                     HLLGAAVSSKHDWSRLHSVVTNAAYVYHSRNDAVLSFVYTIVQGGSKAAGSVGAATKL
                     RRIHNVDVTTHVAGHSAYCTSVDLRGAG"
     gene            complement(788..1201)
                     /locus_tag="PLQ54_17590"
     CDS             complement(788..1201)
                     /locus_tag="PLQ54_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95132.1"
                     /translation="MLALLGTVGGASDYVNKRERERPARSVEEEPAHIAKGVRRGNDQ
                     GMASIHECWRRITEHEVAPFRTKSGLPFTYSVVGNNLWITGSGREINRGLSRHNVDRA
                     LREMPAARPGELKAVQGSAYVWAILMDDRIRASDW"
     regulatory      1261..1379
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00634"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class IV; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00634"
BASE COUNT          302 a          577 c          573 g          298 t
ORIGIN      
        1 gagaagctca aggatggccc gggtattccg gtcgtcgtct gctcgggatt cctcaccgag
       61 aaggtcaagg gttggggcga ctgggaacgc atcgtcaccg agcgctaccc cgactccccc
      121 gtgtttcggg ttcactgggg agccgaggag ttgcaggatc tgtatgccgt gatctccggc
      181 aacgctggca aggccgcctt ggccaagttc gctggaaagg cggtcttgaa ggccagcaag
      241 caggcggcca aaaaggccgg ccccattggt ggcgcgctcg tcgtggtcga cctcgtgaag
      301 aacccatggt ggcgagcccg agaacgcgcg aacaagaccg gcgccattgt cgccgagctc
      361 attgcgcgca cttcggttga cgaggtcgtg ctggtcgggc acagtctcgg cgctcgcgta
      421 atgctgtatg ccgcggatgc cctcagtctc aagagcgacg ctccgcgggt gcgcgacgtg
      481 cacctgctgg gagctgcggt gtcgtcgaag cacgactgga gccggctcca ttcagtggtg
      541 accaatgcgg cttacgttta tcactctcgc aatgacgctg tcctcagttt cgtctacaca
      601 atcgtccaag gggggtccaa ggctgccggt tcagttggag ccgcgaccaa gcttcggcgg
      661 attcacaacg tcgatgtgac cacgcatgtc gcgggccatt cggcgtactg cacaagtgtt
      721 gatctacgcg gggccggctg agcatgagcc atgcggtgga ggcaactacc gcgatgtccc
      781 agcgtcgtca ccagtcgctg gcacggatcc tgtcatccat gaggatggcc cagacatagg
      841 cagaaccttg caccgctttc aactcgccgg gtcgtgcggc tggcatctct ctcagtgccc
      901 ggtcaacgtt gtggcgagag agcccacggt tgatctcgcg gccagacccg gtgatccaca
      961 ggttgttccc caccacggag tatgtgaagg gaagcccaga cttggtgcgg aatggagcca
     1021 cctcatgttc cgtgatccgt cgccagcatt catgaattga ggccatgccc tgatcgttcc
     1081 cacgtcgaac tcccttggca atatgtgcgg gctcttcctc cacggatcgg gcaggacgtt
     1141 cgcgctctcg cttgttgacg tagtcggagg cgcccccgac ggtgcccaag agggcgagca
     1201 tccgttgcga caatcgacga gtccaatggc tgtcggagcc ccctgctagc tcccgggcaa
     1261 ggtcaagaac ccagcccaca cgcctccgga cgcgggttgg cggcaacagc gtcacccgac
     1321 acggtgccct ccggagaaga ccgggtccgc agccgaccgg ttgcggtcac gccgggagga
     1381 gaaccaccat gcccacccca gccgtcctct gcacgacgcc cgcgtgccga tccctcgcgc
     1441 cgggccacac catgcacccg ttccacgcga ggcgacctgt cacctcggtc ctgtggcccc
     1501 accgcgagct cggcgaccgc ttggccccgg ggtcgcgggt gagggtgcac gagggggtcg
     1561 ccggatccgg cgagggatgc cgcgcagctc acggcccgac gtcgccctcc cagagccgga
     1621 tcgcgccgca ctggtcgagg aaggcgtccg catcgccggc gtccgagatc gtgacgaccc
     1681 cgtcgtccgg cccaacgccg gcgaggtcgg caggtcgtca cgcggagcga cggccgcagg
     1741 cgaagccgac
//
LOCUS       DAOSPL010001144         1749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_68284_length_1749_cov_3.360094, whole genome shotgun sequence.
ACCESSION   DAOSPL010001144 DAOSPL010000000
VERSION     DAOSPL010001144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68284_length_1749_cov_3.360094"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLQ54_17595"
     CDS             <1..250
                     /locus_tag="PLQ54_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95133.1"
                     /translation="AECVEREFHGSGTSRHEHKGFALQQVLARAGPKPVGPGKQMELS
                     ARLEVPPGGPHSLEARCAGVQWALKADERLAGLGPGCG"
     gene            301..570
                     /locus_tag="PLQ54_17600"
     CDS             301..570
                     /locus_tag="PLQ54_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95134.1"
                     /translation="MPGDPGVAAYGDGNEEKRAITPEQTMHEGALQGGLRARHILGIR
                     VPADGPVSYAGKVVRVTLEVVVLIETPVWRDRRVALPFLVAPRSE"
     gene            complement(582..1418)
                     /locus_tag="PLQ54_17605"
     CDS             complement(582..1418)
                     /locus_tag="PLQ54_17605"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOS95135.1"
                     /translation="MRELLNDATARGYAVPSFCVWNAETARTVLMAAEDLRAPVILMG
                     AWGELLMLPPADMAAVVRAIGARCSVPFSLHLDHGHTLELVNDCLRAGYSSVMLDFSA
                     KPIEENTEALRAVVEMARPHGASVEGELGVVGRVDHVTGEGGTGSTLTDPSMAAEFVA
                     ATGVDALAVSIGNAHGMYHELPRLDFGRLAQLRDAVPVPLVLHGGSGTPDADLRRAIS
                     LGIAKVNVASELVKAMRESLMAQWSTGENLWIPIAQAKAMESFGDVVRRWLGLTAAEG
                     KA"
BASE COUNT          271 a          599 c          621 g          258 t
ORIGIN      
        1 cgcggagtgc gtcgagcgag agttccacgg ctcgggcacg tcgaggcacg agcacaaggg
       61 cttcgcgctc cagcaggtac tcgcccgggc gggcccgaag ccggtggggc ccggcaagca
      121 gatggagctg tcggcccgtc tggaggtgcc tccgggcgga ccccattcgc tcgaggcgcg
      181 ctgcgcgggg gtccagtggg ctttgaaggc cgacgagcgc ctggctgggc tgggcccggg
      241 ctgcgggtga gatggcccgt gacggtcgca ccgtgcgtcg cctccgggag cggaccaggt
      301 gtgcctgggg acccaggtgt ggcggcgtac ggtgatggca acgaggagaa gcgagcgatc
      361 acgccagagc agaccatgca cgaaggggca cttcagggtg gccttcgcgc ccggcatatc
      421 ctgggcatac gggtgcccgc cgatggcccc gtgagctacg caggcaaggt tgtccgggta
      481 acgctggagg tagtggtact catcgaaacc cccgtctggc gcgacaggcg tgttgccctg
      541 ccgttcctcg tggcaccgcg cagcgagtga ccgtcaggtc ttcaggcctt cccctcggcc
      601 gcggtcaggc cgagccatcg gcgaaccaca tcgccgaatg actccatcgc cttggcctgg
      661 gcgatgggga tccagagatt ctcccccgtg ctccactggg ccatgagaga ctccctcatg
      721 gctttcacca gctcgctagc gacgttcacc ttggcgatcc cgagcgaaat ggcccggcga
      781 agatccgcgt ccggcgtgcc gctgccgccg tgcagcacca gcgggaccgg cacggcgtcg
      841 cggagctggg cgaggcgccc aaagtcgagc cgcggcagtt cgtggtacat gccgtgcgcg
      901 ttgccgatcg acacggccag ggcgtcgacg ccggttgccg ccacgaactc ggccgccatc
      961 gacgggtcgg tgagagtgga tcccgtgccg ccctcgccgg tcacgtggtc cacgcggccc
     1021 acgactccga gttcgccctc gacgctggcg ccatggggcc gcgccatctc gacgacggcg
     1081 cgaagcgcct cggtgttctc ctcaatgggc ttggccgaga agtccagcat cacggacgag
     1141 tagcccgccc tcaggcagtc gttgacgagc tccagtgtgt gcccgtggtc caggtgaaga
     1201 gagaagggga ccgagcaacg ggcgcctatg gcgcggacca ccgctgccat gtcggcaggt
     1261 gggagcatca gcagctcgcc ccaggcgccc atgaggatca ccggagcccg gagatcctcg
     1321 gccgccatga gtaccgtgcg ggcggtctcg gcattccaga cacaaaagga cggcacggca
     1381 tagccccgag ccgtcgcgtc gttgagtaac tcacgcatgg gcaccaacgg cacagcagtt
     1441 gcccctctcc tgtcaccaat cgtgtggcaa tcagacacgg cggcgctccg cgcgcgagcg
     1501 tttcggggtg ttcggttcag gcctggcggg acccgccggg ggaacggcgg tgccgtcgtc
     1561 gcatcccgaa aggcgtggcc catctggggg aagctccgcg tggcctcgcc cccatcatac
     1621 acgacggcac caaccaccgc gggtccaaac ctcgtggatc ccaggggcgc tgcgttccgg
     1681 gtgcctcacg gctcgccggg cgcaaccgcc cgagctgggg ccgctgccgc cgcgtcggct
     1741 tccgcccgc
//
LOCUS       DAOSPL010001145         1749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_68311_length_1749_cov_2.346517, whole genome shotgun sequence.
ACCESSION   DAOSPL010001145 DAOSPL010000000
VERSION     DAOSPL010001145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68311_length_1749_cov_2.346517"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="PLQ54_17610"
     CDS             <1..1378
                     /locus_tag="PLQ54_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95136.1"
                     /translation="AALAAILFLVNAEAVSMAISGRPNILTGFLVLAALLALSPRAPR
                     PSVEAAFATSQLPESVLRVFRVFTQPSVRAIAGGALIALCYLTDTFYGWLIVPAALLS
                     RQLAPSRRGWGPVLACLAAFIVLAAPWWIRSYHLTGYPFFSFARYDVLRNTADFPGTQ
                     VLRDFAAPPDPWVYAFTHPMRLVRFALTGLTAAVNGLVVMLGVVMLPFAVLWFFSPAT
                     DPAEGTRRRGVAGFAIAILAVAMTQEAALDRFFPLLPTAAIGASWMLVRLVQRMGALR
                     RRAIMAGAVVVASLTLVSAMLSPAVRVPWTHMVNITMAGAELPRDTLVVSDQPWYVAW
                     HAGLASLWCPKDPAVARSRFLDAQLAKPAALYLSARLATDYPRDPDMIQFWNRARGVQ
                     PPAGYQFAPLRGRLQLPDALWVTDAMAESMTASSPTDRAATEQTGRASTASKTSGPAQ
                     TRPSASGP"
     gene            1465..>1749
                     /locus_tag="PLQ54_17615"
     CDS             1465..>1749
                     /locus_tag="PLQ54_17615"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95137.1"
                     /translation="MSRAEIAETVLLLLSVAALWPWAFLKYRAPWYTAVLVAVLLVMV
                     LLCVRLVRRTSRAFREAERQMREEGASPGVLPWCMPPSAKSRGSGDDPAEN"
BASE COUNT          221 a          583 c          612 g          333 t
ORIGIN      
        1 ggcggcgctg gcggccattc tgttcctggt caatgccgaa gccgtgtcga tggccatcag
       61 cgggcgcccc aacatcctca cagggttcct ggtcctggcg gcgctgttgg ccttgtcgcc
      121 gagggcccct cgccccagtg tcgaggccgc gttcgcgact tcgcagctac ccgagtccgt
      181 gctccgcgtg ttccgggtgt tcacccagcc ctcggtccgc gcgattgccg gaggggcact
      241 gatcgcgctg tgctacctga cggacacgtt ctacggctgg ctgatcgtac ccgctgcgct
      301 gctcagtcgg cagttggctc catcgaggcg tggctggggc cctgttttgg cgtgcctggc
      361 cgcgttcatt gtcctcgccg cgccctggtg gatccgcagt taccatctca ctggctatcc
      421 attcttcagc tttgcccggt acgacgtgct ccgcaacacg gcggacttcc caggcaccca
      481 ggtgcttcgc gacttcgcgg cgccgcccga tccatgggtc tacgcgttca cgcacccgat
      541 gcggctcgtg cgcttcgccc tgacgggact gacggcggcg gtcaacggcc tggtcgtcat
      601 gctgggcgtg gtgatgcttc cctttgccgt gctgtggttc ttctctccgg cgaccgatcc
      661 ggccgagggg acgcgccggc ggggtgttgc cgggttcgcc atcgcgatct tggcggtggc
      721 catgacccag gaggcggcgc ttgaccgttt ctttcccctc ctgccaaccg ccgcgatcgg
      781 ggcgtcgtgg atgctggtgc gtctggtcca acgcatgggt gcgctccggc gccgggcgat
      841 catggccggc gcggtcgtgg ttgcctccct gactctggta tccgccatgc tctcgccggc
      901 ggtccgggtg ccgtggaccc atatggtgaa catcacgatg gccggtgccg aactgccccg
      961 ggacaccctg gtcgtttccg accagccatg gtatgtcgct tggcacgcgg ggctggcatc
     1021 gctgtggtgc ccgaaggacc ccgcagtcgc ccggagccgg ttcctcgatg cgcagctcgc
     1081 caagccggcg gccctgtacc tgagcgctcg cctggcgacc gactatccgc gtgatccgga
     1141 catgatccag ttctggaatc gagcccgagg cgtacagccg ccagctggct atcagtttgc
     1201 gcccttgcgg ggccgcctac agctaccaga cgccctgtgg gtaaccgacg ccatggccga
     1261 gtccatgacg gcctcgtcac cgacggacag ggcggcaaca gaacagacgg gccgggcgtc
     1321 gaccgcgagc aagaccagcg ggccggccca gaccaggcca tcggcctcgg ggccgtagcg
     1381 gcggcagggg cccgaaggtt gggtgtgcgc gcaggccgaa ctgcatcggt gcgctcagga
     1441 cacggggagc gaggcggtca gcagttgagc cgtgcagaga tcgctgagac ggtgctgctg
     1501 ctgttgtcgg ttgcggcgct ctggccgtgg gcgttcctca agtaccgcgc gccgtggtac
     1561 acggcggtgc tcgtggcggt gcttctcgtc atggtgttgc tgtgcgttcg gctggtgcga
     1621 cgaacgagtc gagcgtttcg tgaggccgaa cggcagatgc gcgaggaggg ggcctcgccg
     1681 ggagtcctgc catggtgtat gcccccttcg gccaagtcac gcgggtccgg ggacgacccg
     1741 gctgagaac
//
LOCUS       DAOSPL010001146         1748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_68333_length_1748_cov_3.681630, whole genome shotgun sequence.
ACCESSION   DAOSPL010001146 DAOSPL010000000
VERSION     DAOSPL010001146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68333_length_1748_cov_3.681630"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..757
                     /locus_tag="PLQ54_17620"
     CDS             125..757
                     /locus_tag="PLQ54_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOS95138.1"
                     /translation="MGQLLYIQASPRGDRSYSVSAAHAFIDAYRSAHPDDMVTTLDVF
                     ERDLPPFDGFMLQAKYTVLHGASPTGEERAAWDQVTAIVDEFKAADRYLIATGMWNFS
                     IPYRLKQYLDIIVQPGLTFTYSAERGYQGMVTGKPLALICARGGEYPPGTPGAALDFQ
                     RPYLETVLRFIGFEGIRTVVIEPTLMGGQEAAERARSSALARAAELGAEI"
     gene            complement(770..>1748)
                     /locus_tag="PLQ54_17625"
     CDS             complement(770..>1748)
                     /locus_tag="PLQ54_17625"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HOS95139.1"
                     /translation="SYAGYGFCEAHAAAFADTAYKTAYLLAHYPAEFYAALMSHQPMG
                     FYPPNTLLWEAKRRGIEVLGPDVNRSELGFTVEGGAIRVSLSQIRGMGAIRREQILAE
                     RQAHGPFRSFVDFCRRVRPDHDLGRDLILSGAFDSLCPNRRRLLWELGRVVRDGGSER
                     LDLPGDSTGRQHRVPEFTADEKWWHEFQVLGLGLRRHPMEVLRRNLRGRGVATIAEAT
                     QWKRNGSVRVAGLAVRPHRPPTRSGRTVAFFTLEDETGLMDVTVFESVYQRYGKPLFT
                     RPAVVVTGRKEGRNGSSRPDSMVAMSIQELQFGGHAPASMAPKWSSWVP"
BASE COUNT          307 a          585 c          568 g          288 t
ORIGIN      
        1 ggggcaccct ctggatctgg ggcgccggaa cggtccgcga gggtggggtg ttgcatacca
       61 cagagagtgc gtgaagggta tccgtccgcc cccattcagc acgggatcac cggaggagag
      121 gccaatggga cagctactct acatacaggc gtcaccgcgg ggggatcgct cgtattcggt
      181 ttctgcagcc cacgcgttca tcgacgcata ccggtccgcc cacccggacg acatggtgac
      241 gaccctggat gtcttcgaga gggacctgcc gcccttcgac ggcttcatgc tccaggccaa
      301 gtacaccgtg ctgcacggtg cttcacccac gggcgaggag cgcgccgcgt gggaccaggt
      361 cacggccatc gtcgacgagt tcaaggccgc cgaccgctac ctcatcgcca cgggcatgtg
      421 gaacttcagc attccctatc ggctgaaaca gtacctcgac atcatcgttc aaccgggtct
      481 gacgttcacc tactcggcgg agcggggcta ccagggcatg gtcacaggga agccgttggc
      541 tctgatatgc gctcggggtg gcgagtaccc gccgggcacc ccgggcgcgg cgctcgactt
      601 ccagaggcca tacctggaga cggtcctccg cttcatcggc ttcgagggta tccggaccgt
      661 ggttatcgag cccacactga tgggtgggca ggaggcggcc gagcgggcgc ggtcgtccgc
      721 gctggctagg gctgcggagt tgggagcgga gatctagggc cgggccgggt cacggcaccc
      781 atgaactcca cttcggcgcc atgctcgccg gcgcgtggcc tccgaactgc agctcctgga
      841 tactcatcgc caccatgctg tcggggcggc tgctgccgtt ccggccctcc ttccgtccgg
      901 ttacgacgac ggccggacgc gtgaacaggg gcttcccata gcgctggtag accgactcga
      961 acacggtgac atccatcaac ccagtctcat cttcaagggt gaagaaggcc accgtgcgtc
     1021 cgctcctcgt tggcggccgg tggggacgga cggccaggcc ggccacgcgg acactcccgt
     1081 tccgcttcca ctgagtggct tctgcgatgg tggccacccc ccgacctctc aggttccttc
     1141 ggagtacctc catggggtga cgccgaagcc caaggcccaa cacctgaaac tcgtgccacc
     1201 acttctcatc ggcggtgaac tccggcacgc gatgttgccg cccggtggaa tcacccggga
     1261 gatccagccg ctccgaaccg ccatctctga ccactcgtcc gagctcccac aggaggcggc
     1321 gccggttcgg gcagagcgaa tcgaacgccc cagacaggat caggtcgcgc ccgagatcat
     1381 gatccggacg cacccgccgg cagaaatcga cgaacgaacg gaatgggccg tgggcctggc
     1441 gttcggcgag aatctgctcg cggcggatag cccccatgcc cctgatctgc gagagcgaga
     1501 cgcgtatggc gccgccctcc accgtgaaac ccagctcact ccggttcaca tccggcccca
     1561 gcacctcaat gccgcgccgc ttggcttccc acagtagcgt gttgggcggg tagaagccca
     1621 tgggctggtg ggacatcagg gctgcgtaga actcggccgg gtagtgggcc aagagatagg
     1681 ccgtcttgta ggcggtgtcg gcgaatgcgg ccgcatgggc ctcgcagaaa ccatagccgg
     1741 catacgac
//
LOCUS       DAOSPL010001147         1747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_68395_length_1747_cov_3.776005, whole genome shotgun sequence.
ACCESSION   DAOSPL010001147 DAOSPL010000000
VERSION     DAOSPL010001147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68395_length_1747_cov_3.776005"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..>1747)
                     /locus_tag="PLQ54_17630"
     CDS             complement(169..>1747)
                     /locus_tag="PLQ54_17630"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS95140.1"
                     /translation="MRVLLAKLAFCGCEVSSGAFLVFAIAFAGRRSLLRRPSILALLG
                     VHAISIPLALLLGWSRLTTGDVAAVAADPVALPAGWRPAHGTATLASTLVASGLFAVM
                     AIQVPAHYRSRVRWMAVAALLPLAASMAYAAHAGHEPAIRLIPAGHAASGLLMVLAFH
                     GLGLLDALPLARDLVFMRMPDPVLVMDRRDRIADMNHGAEALLGVPVRRAVGLRAADV
                     ASRFPELAALLASPRAGAGELAVSEPDGARHFAAKVSSVGADEREATGTVVLLHDITD
                     RKEAELALRAAHEELQALEQMRDDLTHMIVHDLRTPLTSVASGLRTLRTVARLDSQEQ
                     ALLDTAIEGGDTLLSMINDLLDISKMEAGALQLRLQEVAPEELIRQACQQVELLARQK
                     GQTLVAEPESGLPSVYADPDLARRTLVNLLGNAVKFTPSGGAVTVTASVAEGEAVVFR
                     VSDTGIGIPEDARERIFDKFGQIESRKTGGRSSTGLGLTLCRLAVEAHGGRIWVDSEP
                     GAGSTFSFALGGGVQSG"
BASE COUNT          297 a          622 c          574 g          254 t
ORIGIN      
        1 cgtattacct gagccagatt gccgccggcc agggcgccac cagcccgtgc gtcgacgccg
       61 gatccaccac atccgcagcc agcggcatgg cgggccggac tacgcgcacc gacggtgtgc
      121 ctgacgccgg caccgtcgat atcggatacc actacgcgcc atagtccctt agccggactg
      181 aacaccgcca cccagtgcga agctgaatgt ggagccggct cccggctcgc tgtcgaccca
      241 gattctgccc ccgtgggcct cgacggcgag ccggcacagc gtgagcccga gccctgtcga
      301 tgagcgcccg ccggtcttgc ggctctcgat ctgaccgaac ttgtcgaaga tgcgctcccg
      361 ggcgtcctcg gggatgccga tccccgtgtc cgacacgcgg aacaccacgg cctcgccttc
      421 agccacgctc gccgttacgg tgaccgcccc cccgctcggc gtgaacttga cggcgttgcc
      481 caagaggttc accaaggtgc gccgggccag gtctggatcc gcgtacacgg agggcagacc
      541 gctctcgggt tcggccacga gagtctggcc tttctgccgt gccaggagtt cgacttgctg
      601 gcatgcctgc cggatcaact cctcgggggc gacttcctgc agacgcagct gcagagctcc
      661 cgcctccatc ttgctgatgt cgagaagatc attgatcatc gagagcagtg tgtcgcctcc
      721 ctcgatagct gtgtcgagta gcgcctgctc ttggctgtcc agccgtgcaa ccgtcctgag
      781 ggtgcggagc cccgaggcca ctgatgtcag cggagtgcgc aggtcgtgga cgatcatgtg
      841 ggtcagatcg tcccgcatct gctccagcgc ctggagctcc tcatgagcgg cccggagtgc
      901 cagttcggcc tccttgcggt cggtgatgtc gtggaggagg acgacggtgc cggtcgcctc
      961 acgctcatcg gcgccgaccg acgagacctt ggccgcgaag tgtctggccc catccggttc
     1021 gctcacggcg agttcgcctg ccccggcacg cggcgatgcc agaagcgccg ccagctcagg
     1081 aaaccgcgat gccacatccg ccgcgcggag tcccaccgcg cgccgcacag ggactccgag
     1141 gagcgcctcc gcgccgtggt tcatgtccgc gatacggtcc cggcggtcca tcacgagcac
     1201 ggggtcaggc atgcgcatga acacgagatc gcgcgccagc ggcagcgcgt cgagcaaacc
     1261 cagaccgtgg aaggccagca ccatcagcag gccgctcgcc gcgtggccgg cggggatcag
     1321 ccgaatggcc ggctcgtgcc ccgcgtgcgc cgcatatgcc attgaagccg ccaagggcaa
     1381 cagcgccgca acggccatcc atctcacgcg gctccggtag tgggccggca cctggatcgc
     1441 catcacagca aacaggccgg atgccaccag cgtcgaggcc agggtggccg tgccgtgggc
     1501 cggccgccag cctgccggca gtgccaccgg gtcggcagcc accgcggcca cgtcgcccgt
     1561 cgtcagccgt gaccaaccga ggagcagtgc aagaggaatc gagatggcgt gcacgccgag
     1621 cagagccagg atcgagggac gccgcaggag tgaccggcgc ccggcgaagg cgatggcaaa
     1681 gacgaggaat gctcccgagg acacctcgca gccgcagaat gccagcttgg ccaacagcac
     1741 ccgcatc
//
LOCUS       DAOSPL010001148         1742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_68695_length_1742_cov_2.979846, whole genome shotgun sequence.
ACCESSION   DAOSPL010001148 DAOSPL010000000
VERSION     DAOSPL010001148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68695_length_1742_cov_2.979846"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1742)
                     /locus_tag="PLQ54_17635"
     CDS             complement(<1..>1742)
                     /locus_tag="PLQ54_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95141.1"
                     /translation="RPMGTSLALHAALVAAVSGPCAAYDVLVAMHDGGSIECYDGATG
                     SHRGTFAVGIDNPNCLAWGPDGTLFVSTGALNGPGGVERFDPATGEPLGYLVPPAASG
                     AGRLARAVGMAFRGGKLYVASCDDGRIVCYDAPSGKWLSDFVAQSIRRFDANAGAPLP
                     DLTAIAGYSPWGLALDSSGRLFWSGSGSGGHTIQCLAGATNQHWAGPHATLNAPVQLA
                     LAPDGLLYCSSYFGRNVTVWSTDHPSAGDPIHVIRGPEVRGPNGIVFTERPVPMRPAP
                     IELESSTKSACVRAQAEPDHAALPYLSWDTEGGDRARTNLLRAPVRLRLVGRDGARPS
                     VEARRRGSHGITYRLGLPSGGEVSWDVALRGGGISMTFGATGTELGHVRGIEIEFPLD
                     PRATATTVLTGDWSRAGRARLPFIISAPDLGQMYVSCAEHPDVEAALEGDRQAQTVTL
                     VFHLPVPESGTAYTLDFRPHVLAVPGGLEDTARWRDARRGWFNFLQLSSTWCGQPGIW
                     ANNVVSDPVSSTVFWLADHALLLPELAPGVSTVAMVRRSVEYWLDGGRTPEGEFYYVV
                     NSTPGMMDSYPSIL"
BASE COUNT          282 a          615 c          616 g          229 t
ORIGIN      
        1 atgaggatcg aggggtacga gtccatcatc ccgggcgtcg agttcacgac gtaatagaac
       61 tctccctccg gcgtccggcc gccatcgagc cagtactcga ctgatcgccg caccatggcc
      121 accgtcgaca cgcccggcgc cagctcgggc aagagcagcg cgtggtcggc cagccagaac
      181 acggtggagc tcaccgggtc actgaccacg ttgttggccc agatccccgg ctgcccgcac
      241 cacgtggatg agagctggag gaagttgaac catccccgcc gggcatcgcg ccaccgcgcc
      301 gtgtcctcga gccctcccgg caccgcgagc acatgagggc ggaagtccag cgtgtaggcc
      361 gtgccagact cgggcacggg taggtggaac accagtgtta ccgtctgggc ctgccggtca
      421 ccctcgaggg cagcctcgac atccgggtgc tcggcacacg agacgtacat ctggcccaga
      481 tcgggcgcgc tgatgatgaa cggcagccgg gcgcgaccgg cccggctcca gtcgcccgtg
      541 agcaccgtcg tcgccgtggc gcgcgggtcg agggggaact cgatctcgat cccgcggacg
      601 tggcccagct cggttcccgt ggcgccgaag gtcatcgaga tgccgccgcc ccggagggcc
      661 acatcccacg agacctcacc ccccgagggc aggcccaggc ggtaggtgat gccgtggctc
      721 cctcggcgcc gcgcctcaac gctcgggcga gcaccgtcgc ggccgacgag gcgcagccgc
      781 acgggcgcgc ggagcaggtt ggtccgcgcc cgatcgcccc cctcggtgtc ccagctcagg
      841 taggggaggg cggcatggtc aggctcggcc tgcgctcgga cgcaggccga tttggtcgac
      901 gactcgagct cgatgggtgc gggacgcatg ggcaccggcc tctccgtgaa cacgattccg
      961 ttcggcccgc ggacctcggg gccacggatc acgtggatgg gatcaccggc cgaggggtga
     1021 tccgtcgacc acaccgtcac gttgcggccg aagtagctgg agcaatagag aagcccgtcg
     1081 ggcgccaacg ccagttgcac aggcgcgttg agcgtggcat gggggccggc ccagtgctgg
     1141 ttggtcgcgc cagcgaggca ctggatcgtg tgcccgccgc tcccgctgcc gctccagaac
     1201 aggcgtccgg agctgtcgag cgcgaggccc cagggcgagt agccggcgat ggccgtgaga
     1261 tcgggcaggg gcgcgccggc gttggcatcg aaccggcgga tcgactgggc aacgaagtcg
     1321 gacagccact taccgctcgg cgcgtcgtag cacacgatgc gcccgtcgtc gcagctcgca
     1381 acgtagagct tcccgccgcg gaatgccatg cccacggcgc gagccaaccg gcccgctccc
     1441 gaggcggctg gcggcaccag ataccccagg ggctcgcccg tcgccgggtc gaaccgctcc
     1501 accccaccgg gcccgttcag cgcgccggtc gagacgaaga gcgtgccatc gggtccccag
     1561 gcgaggcagt tcgggttgtc gataccgacc gcgaaggtac cccggtggct gcccgtggcc
     1621 ccgtcgtagc actcgatgga gcccccatcg tgcatggcca ccaggacatc ataggccgcg
     1681 caggggcccg agacggcggc aaccagagcc gcgtgcaagg ccagcgatgt gcccatggga
     1741 cg
//
LOCUS       DAOSPL010001149         1742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_68696_length_1742_cov_2.917605, whole genome shotgun sequence.
ACCESSION   DAOSPL010001149 DAOSPL010000000
VERSION     DAOSPL010001149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68696_length_1742_cov_2.917605"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PLQ54_17640"
     CDS             <1..670
                     /locus_tag="PLQ54_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95142.1"
                     /translation="GVTEAMLDLHDRPEMVHAAVDRMVAAWMTELDQFVEQNLLSLDC
                     NNTRVGSGGYGYTRELPGSDFDPGWVRPHNMWGCSNAQAFSQVSPETHWEFALKHEIP
                     WLSRWGLNYYGCCEPLDAKMDILRRIPRLRKISMSCWVDLDRAVRNVGRDFVISYKPN
                     PAYLAEHRWRPEAMRDELRTALEKTRGCHVEIILKDISTVRYQPQRLWEWAAMASELV
                     SEQA"
     gene            736..>1742
                     /locus_tag="PLQ54_17645"
     CDS             736..>1742
                     /locus_tag="PLQ54_17645"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOS95143.1"
                     /translation="MTGVSKRFPGSLAVDHVDFEARVGEVHALMGENGAGKSTLMRIL
                     AGSFDDYTGQVEVGGRPVTLHSPAAARDCGIAMIYQELSLARKRSIAENVLAGRLPCR
                     RFGWLDTGAMLEQARACLARVGLDLDPRTPVEEISQHEAQLVEIARALGHVPCILVMD
                     EPTSALSREEVDRLFEIILNLKRTGLSIVYISHHLPEVFRVADRVTVLRDGKRVGTYE
                     IGDVTHESLIEAMVGGAASEHQTHRGSNLGAPLLHAEHLSRYGFFHDVNLTVHEGEIV
                     GIAGLNGAGRTELARAMCGIDPLDEGRVVLNGEALRPRNAEEAIRRGLAYLTEDRKTQ
                     GL"
BASE COUNT          303 a          585 c          573 g          281 t
ORIGIN      
        1 gggggtcact gaggccatgc tcgacctgca cgaccgtcct gagatggtgc atgctgccgt
       61 ggatcgcatg gtagcggcgt ggatgacgga gctcgaccag ttcgtcgaac agaacctgct
      121 gtcgctggac tgcaacaaca cgcgcgtcgg ctccgggggc tacggctaca cccgggagct
      181 gccggggagc gacttcgatc ccggctgggt ccggccccat aacatgtggg gctgctcgaa
      241 cgcccaggct ttctcccagg tgtcgcccga gacccactgg gagttcgccc tcaagcacga
      301 gatcccatgg ctctcgcgct gggggctcaa ctactacggg tgctgcgagc ccctcgacgc
      361 caagatggac atccttcgcc ggatccctcg tctgcggaag atctcgatga gctgctgggt
      421 cgatctggat cgcgccgtcc gcaatgtggg ccgggacttc gtcatcagct acaaaccgaa
      481 ccccgcctac ctggcggagc accgctggcg gccggaagcc atgcgggacg agctccgcac
      541 ggcgctcgag aagacccgcg gatgccacgt ggagatcatt ctgaaagaca tctcgacagt
      601 ccgctaccag ccccagcgcc tgtgggagtg ggcagccatg gcctccgaac tggtcagcga
      661 gcaggcttga gcgccggaga cctcgaagcc cactcgtggt gaggtgcgat ggcccacgag
      721 accccggcgc ttcgcatgac aggcgtctcc aagcggttcc ccggctccct ggccgtggat
      781 cacgtcgact tcgaggcgcg ggttggcgaa gtccacgcgc tcatgggcga gaacggtgcc
      841 ggcaagtcga cactcatgcg aatcctcgct ggctccttcg acgactacac tggccaggtc
      901 gaagtcggtg gccgcccggt taccctgcac tcgcctgccg cggcccgaga ctgcggcatc
      961 gccatgatct accaggagct cagcctggct cgcaagagga gcattgccga gaatgtcctg
     1021 gcaggccgcc tgccttgccg ccgcttcggg tggctcgaca ccggagccat gctcgagcaa
     1081 gcccgtgcgt gcctggcccg cgtcggcctc gacctcgacc cgcgaacgcc ggtggaggag
     1141 atcagccagc acgaggccca actggtcgag atcgcgcgcg ccctgggcca cgtgccatgc
     1201 atactggtca tggatgagcc cacttcggcc ctgtcgcgcg aggaggtcga ccggctgttc
     1261 gagatcatcc tcaacctgaa gcgcacgggt ctctcgatcg tgtacatttc ccaccacctt
     1321 cctgaggtgt ttcgcgtcgc ggatcgcgta acggtcctcc gcgatggcaa gcgcgtcggc
     1381 acatacgaga tcggagatgt gacccacgag tcgctgatcg aggccatggt cggcggcgcg
     1441 gcgagcgaac accagacgca ccgcggcagc aacctgggcg cgccgctgct ccacgcggag
     1501 cacctcagcc ggtacggctt cttccacgac gtgaacctca cagtccacga gggagagatc
     1561 gtcggcatcg cgggtctcaa tggggccgga cgcaccgagc tggcgcgcgc aatgtgtggg
     1621 attgacccac tcgacgaggg ccgcgtggtc ctgaacgggg aggcgttgcg tcccaggaac
     1681 gccgaggagg caatccggcg cggtctggcc tatctcactg aggaccgcaa gacccagggc
     1741 ct
//
LOCUS       DAOSPL010001150         1742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_68698_length_1742_cov_2.820391, whole genome shotgun sequence.
ACCESSION   DAOSPL010001150 DAOSPL010000000
VERSION     DAOSPL010001150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68698_length_1742_cov_2.820391"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLQ54_17650"
     CDS             complement(<1..217)
                     /locus_tag="PLQ54_17650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95144.1"
                     /translation="MRRTSVSVPLGYAERRPAVWYKFVLYPLIAIWLLWFFLGDTIQA
                     TLRERQRHRPSIPAVRVWIPENRPFRRY"
     gene            416..1435
                     /gene="dusB"
                     /locus_tag="PLQ54_17655"
     CDS             416..1435
                     /gene="dusB"
                     /locus_tag="PLQ54_17655"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOS95145.1"
                     /translation="MARGDAAWAPLRIGAVVVDPPTVLAPLAGVTVPPFRRLCRELGA
                     GMVCSEMVSANALAYQNPKTALMLHVCDDEHPVSVQILAPTPEVAEPAAEAAVEAGAD
                     VVDINMGCPVPKVIRCGAGVALMRDLPRARAVMEAAVRGVAGRAPVTVKIRAGWDPRS
                     RCFAEIARQAEDAGLSGIVLHARYGCQGYSGHADWSMIADLRQQTSLPVVGNGDVRGA
                     SDAARMLRETGCDGVMVGRGAMGYPWVFRELRAAARSEAVPEPAGPGERIGALLRLGR
                     EMVDFYGEYRGLRELRGFVCWFTRGMPSSAALRAEALQLTSFEDLRGLLLRYGESWAV
                     RDAEE"
     gene            1444..>1742
                     /locus_tag="PLQ54_17660"
     CDS             1444..>1742
                     /locus_tag="PLQ54_17660"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOS95146.1"
                     /translation="MWEAPETIGRYRIIREIGAGGMGVVFLAEDQVLQRQVALKVLRP
                     QPGLTPEKQAALAERFLRECRVAGRLTHPNIAKVYDAGEDNGCLFLTMELVHGDSL"
BASE COUNT          273 a          556 c          636 g          277 t
ORIGIN      
        1 catagcggcg gaaggggcgg ttctccggta tccagacgcg gactgcgggg atagatgggc
       61 gatgccgctg gcgctcgcga agcgtggcct gaatggtgtc gccgaggaag aaccacaaca
      121 gccaaatggc tatcagcggg tacaggacga acttgtacca tacggcaggc cgccgctcgg
      181 catagcccag cggcaccgaa actgacgtgc gacgcatgcg ccgcttccgc ggccgtttcg
      241 gaagtggaat atagtacatg ggccccccgc gtcgccaccc ggctccggcc gaccgtcacg
      301 agcgcgacac gctgtgatct tttcctgcgc ggtcgcgggg tccttcccac ctgaacaggg
      361 agggcccacc ggcgccgcca ctcgaacatg accgacggcg aggagcgatg gcccgttggc
      421 acgaggcgat gcggcatggg cacccctgcg gatcggtgcg gtggtcgtcg acccgccgac
      481 ggtcctcgca cccctggccg gagtgacggt gccccccttc cggcgcctct gtcgggagct
      541 gggcgccggg atggtctgca gtgagatggt cagcgccaat gccttggcgt accagaaccc
      601 caaaacggcg ctcatgttgc atgtgtgcga cgatgagcac ccggtgagcg tccagatcct
      661 ggccccgacg ccggaagtcg cggagcctgc ggccgaggct gcggtcgagg ccggggcgga
      721 cgtcgtggac atcaacatgg ggtgccccgt accgaaggtg attcggtgcg gtgccggggt
      781 ggccctgatg cgggatctgc cccgggctcg agccgtgatg gaggctgccg tgcggggcgt
      841 cgccggtcgg gcccccgtca ccgtcaagat ccgcgccggg tgggatcccc ggagccgatg
      901 cttcgccgag atcgcccggc aggccgagga cgccgggcta tccggtatcg tcttgcacgc
      961 ccggtatggg tgccaaggct actcgggtca tgccgactgg tccatgatcg ccgacttgag
     1021 gcagcagacc tcgctgcccg tggtcgggaa cggtgatgtt cgaggcgcgt ccgacgccgc
     1081 gcgcatgctc cgtgagaccg gatgcgacgg ggtcatggtt ggtcgcggcg ccatggggta
     1141 cccttgggtc ttccgggagc tccgcgccgc ggcgcggagc gaagccgtgc cagagcccgc
     1201 cgggcccggg gagcgcatcg gcgcactgct ccgactcggg cgggagatgg tcgacttcta
     1261 tggtgagtac cgcggtctca gggagcttcg agggttcgtg tgctggttca ccaggggaat
     1321 gccatcgtcg gccgcactgc gagccgaggc cctgcagctc acgtcgttcg aagacctgcg
     1381 cggcctgctg ctgagatacg gggaatcctg ggctgttcgc gacgcggagg agtaggccac
     1441 gccatgtggg aggcgcccga gaccatcgga aggtaccgca tcatccgcga gatcggcgcg
     1501 ggcggcatgg gcgtcgtgtt cctggccgag gaccaagtgc tccagcgaca agtcgcgctg
     1561 aaggtcctgc ggccgcagcc cggcctgacc ccagagaagc aggcggccct ggcggagcga
     1621 ttcctgcggg agtgtcgtgt tgccggcaga ctgacccacc cgaatatcgc caaggtctat
     1681 gatgccggcg aggacaacgg ctgcctgttc ctcaccatgg agttagtgca tggtgactcc
     1741 ct
//
LOCUS       DAOSPL010001151         1742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_68701_length_1742_cov_2.790160, whole genome shotgun sequence.
ACCESSION   DAOSPL010001151 DAOSPL010000000
VERSION     DAOSPL010001151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68701_length_1742_cov_2.790160"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PLQ54_17665"
     CDS             <1..955
                     /locus_tag="PLQ54_17665"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOS95147.1"
                     /translation="DLRSGLYRVAVVPAGGEAAHRRTVSQIAAQSGAIAACNGGYFAP
                     DNGWPLGLMMCNKEWYRAPMMNRTALLILDDGQIAIGNVTFTGQVQLGAGATLRVNGL
                     NEWLPQDGSSAVVIHTRRWGSAFTTRGSATFAVVRQGRVASVIDAKSSTQVPIPDDGF
                     VVSAVGAQPRAAVKAAQVGAPVAVSLRLDPEVPRVRDALGGGPRLVYRGQQWVTKQVE
                     RFRPDVADSRAPRTALGLTADGRVLLVAVDGRQPGYSAGLLLEELAQLMISLGAVEAM
                     AFDSGGSTALTVAGQVANSPSDGRERPVPNAVAILPVRGGA"
     gene            1033..1608
                     /locus_tag="PLQ54_17670"
     CDS             1033..1608
                     /locus_tag="PLQ54_17670"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOS95148.1"
                     /translation="MDSDAVDLREVTLSSETIFSGRVVSLRVDTVRLPGGRTSTREVV
                     AHPGAVAIVPLTDDGQIVLIRQYRQAAGQVLLEIPAGTLDKGEAPEACARRELAEETG
                     LLAGRLVRLIGSYLAPGYSSELLHVFLGVDLGPADGPDVMDADENIETLTVPVAEAVE
                     WVLDGRVCDAKTICGILLADRWLRHHGGEER"
BASE COUNT          263 a          543 c          648 g          288 t
ORIGIN      
        1 ggatctgcgg agcggcctct accgcgtcgc ggtcgtgccg gcaggcggtg aggcggccca
       61 tcgtcgcacg gtctcacaga tcgcggccca atcgggtgcc atagcggcgt gcaacggcgg
      121 atacttcgct ccggacaacg gatggccgct gggcctgatg atgtgtaaca aggaatggta
      181 ccgcgcgccc atgatgaacc gcacggcgct gctgattctc gatgacgggc agatcgccat
      241 cggcaatgtg actttcacgg ggcaggtgca actcggggcg ggcgccacgc ttcgcgtcaa
      301 cgggctgaat gagtggctgc cccaagacgg ctcatcggcc gtggtgatcc acacgcgccg
      361 ttgggggagc gcattcacca ccaggggctc ggccacattc gcggtggtgc gccaaggacg
      421 agtggccagt gtgatcgacg ccaagtccag cacacaggtc cccatccccg atgatgggtt
      481 cgtggtcagt gcggtgggcg cccagccgcg ggcggcagtc aaggcggcgc aagtgggggc
      541 gcccgttgcc gtgagcttgc ggctcgatcc tgaggtcccg cgggtgcgcg atgcgctggg
      601 cgggggaccg cggctggtgt accgtggtca gcagtgggtg accaagcagg tggagcgctt
      661 tcggccggac gtggccgact cgcgtgctcc gcgcacggcg ctgggcctga cggccgacgg
      721 acgagtgctc ctggtggccg tcgacgggcg ccagcccggc tacagcgcgg gtctgctgct
      781 cgaggaactc gcccagctta tgatctcgtt gggtgccgtt gaggctatgg ccttcgactc
      841 gggtgggtcc acggccctca cggttgccgg ccaggtggcg aactcgccgt cggatggccg
      901 cgagcgccct gtgccgaacg cggtggccat tctgccagta cgcgggggcg cttgagcaac
      961 aaacgccccc ggtgccgttc gggagacggc ggccagcgtc agctctggtc gcgggaagga
     1021 tggcccgaaa ccatggactc cgacgcggtt gacctgcgcg aggtgacttt gtcgagcgag
     1081 accatcttca gcggccgagt ggtgagtctt cgtgtcgaca cggtgcggct gcccggtgga
     1141 cggacatcga cccgcgaggt cgtcgcgcac cctggcgccg tggcgatcgt gccgctgacg
     1201 gatgacggtc agatcgtctt gatccggcag tatcgccagg ccgcgggtca ggtgctgctt
     1261 gagataccgg ccggaacgct ggacaagggc gaagcccccg aggcatgtgc tcgccgcgaa
     1321 ctggcggagg agaccggcct cctggcgggg cgtctggtgc gcctcatcgg gtcctacctg
     1381 gctccggggt actcgagtga gctcctacat gtcttcctgg gtgtggacct gggcccggcc
     1441 gatgggccag acgtgatgga tgccgacgag aacatcgaga ctctgacggt tccggttgcc
     1501 gaggcggtcg aatgggtgct cgacggccgc gtgtgcgacg ccaagaccat ctgtggcata
     1561 ctcctcgctg accggtggct ccgtcatcac ggcggtgagg agcgatgacc cgggagcagc
     1621 tgcacgacct actcgagtcg gtggcggcgc gcgagacggg agtggacgac gccctcgagc
     1681 gcctggcggc gttgccgtac atcgatctgg gtcacaccaa gctcgacacg caccgggccc
     1741 tg
//
LOCUS       DAOSPL010001152         1741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_68780_length_1741_cov_2.379004, whole genome shotgun sequence.
ACCESSION   DAOSPL010001152 DAOSPL010000000
VERSION     DAOSPL010001152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68780_length_1741_cov_2.379004"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PLQ54_17675"
     CDS             <1..843
                     /locus_tag="PLQ54_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95149.1"
                     /translation="AEAVKEANPEAIVIFGGTAGSTLDYPRIALEEGAGPLIDVFAFH
                     PYGHPTPESAPDNFLVLVGENMEWRPRPEAIRTYEDEIAAMRELLHQYNPRMQIWADE
                     MNWFAPGEPPRTDLGDQGELAQAKHLSRFFALNAWLECGAVWWSMYNANGVQEWAVVR
                     SSDMSPRAAYYAAQYASTVLDDVRPAPDVRPEVFVDTPDDLLVKPFRNGRGELLIGLW
                     RTTPAVDACHAEPVTLRLPGVRDRRVELLDLIYGPRQPARIEPDGTLRDLLVGDWPLV
                     VRVR"
     gene            complement(1054..>1741)
                     /locus_tag="PLQ54_17680"
     CDS             complement(1054..>1741)
                     /locus_tag="PLQ54_17680"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOS95150.1"
                     /translation="ALENLMHDLNAAIDGAFAGGAEHVTVLDSHGGGGNFILDMLDPR
                     AENDTKPNGKWWGIMDESYHGTFFIGAHAMAGTMNGFLDHTQSSASWFEYSVNGRPMG
                     EIGQWAIVSGHFGIPALMVSGDERACAEAHDFFHPIETAAVKRGIGRNRAELVEVTEA
                     RSRIREAARRAMAHVGVAKPFVPIKPMEIILRLYRSDYADAIATHEGVERIDARTVRK
                     VTSNALDLFP"
BASE COUNT          274 a          593 c          604 g          270 t
ORIGIN      
        1 gcagaggccg tgaaagaagc gaaccccgag gccatcgtca tcttcggcgg caccgccgga
       61 tcgacgctgg actacccgcg catcgcgctc gaggagggcg ccgggccgct catcgacgtg
      121 ttcgcctttc atccgtacgg ccacccgacg ccggagagcg cgcccgacaa cttcctggtc
      181 ctcgtcggcg agaacatgga gtggcggccg cggcccgagg ccattcgaac gtacgaggac
      241 gagatcgccg ccatgcgcga gctgttgcac cagtacaacc cgcggatgca gatctgggcg
      301 gatgagatga actggttcgc gcccggggag ccgccgcgaa ccgacctcgg cgatcagggc
      361 gagctggcgc aggccaagca cctctcgcgc ttcttcgccc tgaacgcctg gctcgagtgc
      421 ggagccgtgt ggtggtccat gtacaacgcc aacggcgtgc aggagtgggc cgtggtgcgc
      481 tcgtccgaca tgagcccgcg ggccgcctac tacgcggccc agtacgcctc cacggtgctc
      541 gacgacgtgc ggcccgcacc ggacgtacgg cccgaggtct tcgttgacac gccggatgac
      601 ctgctcgtga aacccttccg taacggccgt ggcgagctac tcatcggcct gtggcgcacg
      661 accccggccg tcgacgcctg tcacgccgaa ccggtgacgc tgaggctgcc gggcgtgcgc
      721 gaccggcgcg tggagctgct ggacctgatc tatggccccc gtcagccggc gcgcatcgaa
      781 cccgacggca ccctgcgcga ccttctggtc ggcgactggc cgctggtggt gcgggtgagg
      841 tagggagcgg agaaaccggg agtcaggggc cacaaggtcg gcgactgatc gcggaacact
      901 ggcgcggggc gctcggcctg tgggcgggca ctcaggggcg gggatccgcc tcaggcaccc
      961 gtgggtctgg ctgcagaggg gcgccataag gaggaaccac gcggtcctcg gtatgtcccc
     1021 ggccggccag gggcccggcg cccgcgaacc gccctacggg aacaggtcca gcgcgttgct
     1081 cgtgaccttc cggactgttc gtgcatcgat ccgctcaacg ccctcgtgcg tcgctatggc
     1141 gtcggcatag tccgatcggt agagccggag gatgatctcc atgggcttga tgggcacgaa
     1201 gggcttcgcc acgcccacgt gtgccatggc tcgccgggcg gcctcacgga tgcgggaccg
     1261 agcctcggtg acctccacca gctcggcccg gttccggccg atgccgcgct tcacggccgc
     1321 cgtctcgatg gggtggaaga agtcgtgcgc ctcggcgcac gcccgctcat cgcccgagac
     1381 catgagcgcc ggaataccga agtggcccga cacaatggcc cactggccga tctcgcccat
     1441 gggccggccg ttgacggagt actcgaacca ggacgccgac gactgggtgt ggtccaggaa
     1501 tccgttcatg gttccagcca tggcgtgggc gccgatgaag aacgtgccgt ggtagctctc
     1561 atccatgatg ccccaccact tgccgttggg cttggtgtcg ttctcggccc gagggtcaag
     1621 catgtcgagg atgaagttcc cgccgccgcc gtggctgtcc agcaccgtca cgtgctccgc
     1681 accaccagcg aaggcaccat cgatggccgc gttgaggtcg tgcatgaggt tctccagcgc
     1741 c
//
LOCUS       DAOSPL010001153         1740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_68828_length_1740_cov_2.924629, whole genome shotgun sequence.
ACCESSION   DAOSPL010001153 DAOSPL010000000
VERSION     DAOSPL010001153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68828_length_1740_cov_2.924629"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLQ54_17685"
     CDS             complement(<1..233)
                     /locus_tag="PLQ54_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95151.1"
                     /translation="MLMLIVTVAAQDVLPAQPIGDKTLVAWVSPASLTQSGGAVLSVG
                     GTSNLFDAIVFGEIAPGRWMAGSNFFQRTQGNQ"
     gene            370..1419
                     /locus_tag="PLQ54_17690"
     CDS             370..1419
                     /locus_tag="PLQ54_17690"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOS95152.1"
                     /translation="MLCMGLLLTPVAAPEALFTDVRVVTIEKGPFGPRGMPGDIEVLG
                     DGSLLLCYTLDGIMSRTSTDGGRTWSDEVVLVPNPAAPSTQGYYCHPSLVRLANGDLL
                     LSYIYGSGAVPYYGHNYYRRSADDGKTWSEQFVMTPCPGYVIVHNDKITRLASGRVIA
                     AAERKKRWPDSNDHGGYVACAFHSDDNAYSWHMSANEVDMDPMEAQEAHIEELRDGRL
                     LMVFRTYNGFLGRAYSADQGANWSAGELVRELPISANACAVTVKRIPSTGDLLLIRTT
                     AGDGGRRTPLVSVISKDEGETWESPRELASDPEDDYGYQSVTFLPNEDVCVLSYHARD
                     GLHVARVRTEWFYGG"
     gene            complement(1429..>1740)
                     /locus_tag="PLQ54_17695"
     CDS             complement(1429..>1740)
                     /locus_tag="PLQ54_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95153.1"
                     /translation="GEGRNEVTMQGLSFAQAHLYRSWQSSIDGLERAGRCPLNAFLFD
                     RLCRTFGYSGLGGRTENEELRMEVHHQLGAIPTVTVSHAEEIRSPNRAVARALDRALG
                     S"
BASE COUNT          283 a          573 c          604 g          280 t
ORIGIN      
        1 ttctggttgc cctgcgtgcg ctggaagaag ttgctgcccg ccatccagcg cccgggcgcg
       61 atctcaccga acacgatggc atcgaagagg ttgctcgtac ccccgacgct caggacggct
      121 cccccgctct gggtcaggct cgccggcgac acccaggcca ccagtgtctt gtcgccgatc
      181 ggctgagcgg gtaggacgtc ctgagcggcc accgtcacga tgagcatcag catcgggagc
      241 tcacctccgc agatctgcct gttccgggac gcggcagcgc caacctgcgt gggcggaggc
      301 gcgaagggcc tccgtggggc acccgcgaac cagaccccaa cgcggacact ggaggaggct
      361 gggccattga tgctctgcat ggggctgctg ctcaccccgg tcgcggcgcc ggaggcgctg
      421 ttcaccgacg ttcgtgtggt gacgatcgag aagggcccgt tcggcccgcg gggcatgccg
      481 ggggacatcg aggtgcttgg cgacggctcg ctcctcctgt gctacacgct cgatggcatc
      541 atgtcgcgca cgtcgacgga cggaggacgg acctggagcg acgaagtggt gttggtgccc
      601 aacccggccg cgccctcgac tcagggctac tactgtcatc ccagtctcgt gcgcttggcc
      661 aacggtgatc tgctgctctc ctacatctac ggcagcgggg ccgtgcccta ctacgggcac
      721 aactactacc gccggtctgc cgacgacggc aagacctgga gcgagcagtt cgtgatgacc
      781 ccctgccccg gctacgtcat cgtgcacaac gacaagatca cgcggctggc ctcggggcgg
      841 gttatcgccg cggccgagcg gaagaagcgg tggcccgact cgaacgatca cggaggctat
      901 gtggcctgcg cgttccactc ggacgacaac gcctattcgt ggcacatgag cgccaacgag
      961 gttgacatgg accccatgga ggcccaggag gcgcacatcg aggagctcag ggacggccgc
     1021 ctgctgatgg tcttccggac ctacaacggg ttcctgggcc gggcgtactc ggccgaccag
     1081 ggcgcgaact ggtcggcggg cgagttggtc cgggagctgc ccatctcggc caacgcgtgc
     1141 gcggtaacgg tgaagcggat cccgagcacg ggtgacctgc tgctcattcg gaccacggcg
     1201 ggcgatggcg gacgccgcac gccgctggtg tcggtcatct cgaaggatga aggagagacc
     1261 tgggagagcc ctcgagagct ggccagcgac cccgaggacg actacggcta ccagagcgtg
     1321 accttcctgc cgaacgagga cgtgtgcgtc ctgagctacc acgcccgcga tgggcttcat
     1381 gtggcgcggg tccgcacgga gtggttctac ggcggatagt cggtgcgctc aagaccccag
     1441 ggcgcgatcg agggctcgtg ccacggcccg gttgggcgag cggatctcct cggcatggct
     1501 cacggtgacc gtcgggatgg cgccgagctg gtggtggacc tccatccgaa gctcctcgtt
     1561 ctcggtgcgt ccgcccaggc cactgtaccc gaaggtgcgg cataggcggt cgaacaggaa
     1621 ggcattgagc gggcatcgcc ccgcccgttc gagtccgtcg atgctgctct gccagctccg
     1681 gtagagatgg gcctgggcga aggacaggcc ctgcatcgtg acctcgttgc gcccctcccc
//
LOCUS       DAOSPL010001154         1739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_68890_length_1739_cov_3.067102, whole genome shotgun sequence.
ACCESSION   DAOSPL010001154 DAOSPL010000000
VERSION     DAOSPL010001154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68890_length_1739_cov_3.067102"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PLQ54_17700"
     CDS             <1..510
                     /locus_tag="PLQ54_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95154.1"
                     /translation="SWAKHCPGAIVISVVGATELIEDRLVLDLDTVLHAHEHRRTPSG
                     GGPDPIHTAPGTRWAQVCITVTDHQAAVVEVPGARHERSPASMGLGDRRQDGGASRLC
                     GLLCILARQGGVLDHSIPAAIQGKQRKYVSRLRLALSSFLPGLDGDPLPYDDVDHCYR
                     TAFRITLAE"
     gene            748..960
                     /locus_tag="PLQ54_17705"
     CDS             748..960
                     /locus_tag="PLQ54_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95155.1"
                     /translation="MGRTRGSYTIVVRASELAEPLPAGSVQAAERVLAKLIARHIIRV
                     VAQDGAPPHAVDGAPVQHACGDGRGR"
     gene            965..1540
                     /locus_tag="PLQ54_17710"
     CDS             965..1540
                     /locus_tag="PLQ54_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95156.1"
                     /translation="MQSAADQRREAIDFGSIPEALKALPNWVLWRFELDARDHPTKVP
                     YQISGRKASSTRPRTWADYGSAEQMLLAHWNDSARPTPPGYYAGLGVMLGSDIYGIDV
                     DHAVADDGELTREAQNIVHTVGSYTERSPSGQGVHVLLWADRLPEGRRRRGNVEIYGP
                     GSPRFFTVTGQHVDGTPEALQYRPELAKTLS"
     gene            complement(1565..>1739)
                     /locus_tag="PLQ54_17715"
     CDS             complement(1565..>1739)
                     /locus_tag="PLQ54_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012824040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylpyrophosphate synthetase"
                     /protein_id="HOS95157.1"
                     /translation="NNSPLEELVVTDTIPLKGDAAICKKITVLSVAPLLGEAMKRIYS
                     HESVSELFLWNEK"
BASE COUNT          303 a          600 c          553 g          283 t
ORIGIN      
        1 tcctgggcga agcactgccc gggcgccatc gtcatctcag tcgtgggcgc cactgagctc
       61 atcgaggacc gcctagtcct cgacctggac accgttctcc atgcccacga gcatcgacga
      121 actcctagcg gcggcggccc agatcccatc cacaccgcgc ccggcacgcg atgggcccag
      181 gtctgcatca ctgtcactga ccaccaggca gcggtcgtgg aggtgccagg ggcaagacac
      241 gagcggagtc cggcctccat gggcttgggt gaccgcaggc aggacggtgg agcgagtcgg
      301 ctctgtgggc tgctctgcat tctggcccgg caaggcggag tactcgacca ctccattcca
      361 gcggcgatcc aggggaagca gcgcaagtac gtgagtcgac tccgcctggc tctgagcagc
      421 ttcctccccg gccttgacgg tgaccctctg ccgtacgacg acgtcgacca ctgctaccgc
      481 acagcgttcc gcatcacatt ggcggagtag catccgccag cgcgtgacat gtcacgcacg
      541 ctcggacgcg gttcccagaa acgttacatt cgctctccgc gcgcgccacc atgccgctcc
      601 ccgcccatgg cgccatgccc cctgcccctc catggccggt ccgcgtgaca tttcaccatc
      661 cctccatgga gggccacgcg ctgagcgcgg gcacgccttc cgcactccgg atagcgccgc
      721 tggcgcatgg acaccgggag acgagcgatg ggcagaacgc gcgggtcata caccatcgtg
      781 gtgagggcgt ccgagttggc agagccgctc cccgcaggga gcgtccaggc cgcggagcgc
      841 gtgctcgcga agctcatcgc gcgtcacatc atccgcgtgg tcgcgcagga tggcgccccg
      901 ccccacgcgg tcgacggcgc tcccgtgcag catgcatgcg gtgatgggcg tggacggtga
      961 gagtatgcag agcgcggcag accagagacg cgaggccatc gacttcgggt cgatcccgga
     1021 ggcgctgaag gccctcccca actgggtgtt gtggcgcttc gagctggacg cgcgggacca
     1081 tcccacgaag gtgccttacc agatcagcgg acgcaaggcg tcgtcgaccc ggcccaggac
     1141 ttgggctgac tacggcagcg cagagcagat gctcctcgcg cactggaacg actcagcgag
     1201 gccgaccccg cctggctact atgctgggct cggcgtcatg cttgggagcg acatctacgg
     1261 catcgacgtg gaccacgctg tcgccgacga cggcgaactg acccgcgaag cccagaacat
     1321 cgtgcatacg gtgggttcct acacagagcg gagcccatcg ggccaaggcg tccatgttct
     1381 gctttgggca gaccgtctcc ccgaggggcg gcgccggcgc ggcaacgtcg agatctacgg
     1441 gcccgggtcg ccgcgtttct tcaccgtgac cggccagcac gtcgacggga ccccagaggc
     1501 cctgcagtac cggcctgagc tcgcgaagac cctttcctga acggttccgc ttgaaaaaat
     1561 ccggctattt ctcgttccag aggaagagct cgctgacgga ttcatggctg tagatccgct
     1621 tcatcgcctc tcccaaaagc ggcgccacgc tcaagaccgt gatcttcttg catatggcgg
     1681 catcgccctt gagcgggatc gtgtccgtga ccaccaactc ttccagagga gagttgttg
//
LOCUS       DAOSPL010001155         1739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_68900_length_1739_cov_2.713183, whole genome shotgun sequence.
ACCESSION   DAOSPL010001155 DAOSPL010000000
VERSION     DAOSPL010001155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68900_length_1739_cov_2.713183"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1739
                     /locus_tag="PLQ54_17720"
     CDS             <1..>1739
                     /locus_tag="PLQ54_17720"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOS95158.1"
                     /translation="LVLGIEDGGDGIACDQGAWADAKVTLVDGSVVWVGDLPIIAGQE
                     PPALATALPFSFTYDGRASADFLGAWTSHREDVDLDGARHKTMLAWTDPATRLEVRCD
                     VVQYRDFPVAEWVLHFANRGEQDTPILEGIEAIDTAFHRRPIERPPLGEFILHRNRGD
                     DLNVHNYEPSAQVVGPNETVRIAAVNGRPTQYVFPYFNLEWQSEGVIAVLGWPGQWAA
                     EFAREGETGLRVRGGQEIVRARLRPGEEIRSPLAVLLFWKGDAVHAQNVWRAWMRDHN
                     LPKPGGKPLGSQWNANLLGQTMEKSDAASQMRFIDGYAAHDLKIDYWWMDAGWYEGSH
                     EHGWPHVGTWQVERTRFPGGLREVSERAHSHGMKSLVWFEPERVVAGTWLWEEHPEWL
                     LGTDGGTRLLNLGHPEARAWVTQHIGDLIESEGIDLYRQDYNIDPLGFWRQGEPQDRQ
                     GITENHYVTGYLAFWDALLDRFPALVIDSCASGGRRNDLETMRRSIPKHRSDYIFEPT
                     SQQCMTYALSSWFPIHGSGTLSMDRYELWSTEAPSNTTAWDVTNPDLPYDELRRFGEE
                     FRLVAALQIEGDF"
BASE COUNT          314 a          564 c          575 g          286 t
ORIGIN      
        1 agctcgttct gggcattgag gacggtggcg acggcatcgc gtgtgaccag ggcgcatggg
       61 ccgacgccaa ggtcacgctg gtcgacgggt ctgtcgtgtg ggtgggcgac ttgcccatca
      121 tagcaggcca ggagccgcct gcgctggcaa cagcattgcc cttttcgttt acctacgatg
      181 gccgcgcgtc agccgacttc ctcggcgctt ggacgagcca ccgagaggac gtcgacctcg
      241 atggggcgcg gcacaagacg atgctggcat ggaccgatcc ggccacacgg cttgaggtgc
      301 ggtgcgacgt ggtgcagtac cgcgacttcc cggtggccga gtgggtgctg cacttcgcca
      361 accgaggcga gcaggacaca cccattctcg agggcatcga ggccatcgac accgccttcc
      421 atcgacggcc catcgaacgt ccaccgctgg gcgagttcat cctgcaccgc aaccgcggcg
      481 atgacctgaa cgtgcacaac tacgagccat cggcccaggt ggtcggaccg aacgagacgg
      541 tgcgaatcgc cgctgtcaac ggtcggccaa cacagtatgt cttcccttac ttcaacctcg
      601 agtggcagag cgagggcgtc atcgccgttc tcggttggcc cggtcagtgg gccgccgagt
      661 ttgctcgcga gggcgagacc ggtctgcgcg tgcgcggcgg gcaggagatt gtccgcgcac
      721 ggctgaggcc gggcgaagag atccgctctc ctctggccgt gctcctgttc tggaaaggcg
      781 acgcggtgca cgcccagaac gtgtggcggg catggatgcg tgatcacaac ctccccaagc
      841 ccggcggcaa gcccctgggc tcgcagtgga acgccaatct gctcggccag accatggaga
      901 agtccgatgc ggccagccag atgcggttca tcgacggcta tgccgcccac gacctgaaga
      961 tcgactactg gtggatggac gccggctggt atgagggctc gcacgagcac ggttggcccc
     1021 atgtaggcac ctggcaggtc gagcgcacgc ggttccccgg cgggctccgg gaagtctcgg
     1081 aacgcgccca cagccatggc atgaaatccc tcgtttggtt cgagcccgag cgggtggtcg
     1141 cgggcacgtg gctgtgggag gaacaccccg agtggctgct cgggaccgac ggcggcaccc
     1201 ggctgctcaa cctgggtcac cccgaggccc gagcatgggt gactcagcat atcggcgacc
     1261 tgatcgagag cgagggcatc gacctctacc ggcaggacta caacatcgat cccctgggtt
     1321 tctggaggca aggcgagccc caggaccggc agggcatcac cgaaaaccac tacgtgaccg
     1381 gctatcttgc tttctgggac gcgctgctcg accgcttccc ggcactggtc atcgacagct
     1441 gcgcctcggg cggccggcgg aacgacctcg agacgatgcg acggtcgatc cccaaacacc
     1501 gaagcgacta catcttcgag ccgacgtcgc agcagtgtat gacctatgcc ctctcatcct
     1561 ggttccccat ccacggatcc ggcacgctgt cgatggatcg gtacgaactt tggagcactg
     1621 aggcgccatc gaacacgacc gcgtgggacg tgacaaaccc cgatctgcct tacgacgaac
     1681 tgaggcgctt cggcgaggag ttccgactcg tcgcggccct gcagatcgag ggcgacttc
//
LOCUS       DAOSPL010001156         1737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_69006_length_1737_cov_3.451249, whole genome shotgun sequence.
ACCESSION   DAOSPL010001156 DAOSPL010000000
VERSION     DAOSPL010001156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69006_length_1737_cov_3.451249"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="PLQ54_17725"
     CDS             <1..998
                     /locus_tag="PLQ54_17725"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOS95159.1"
                     /translation="AEDGELGTLLGAETREIEVTFHTLARGVYRVDEAELETTDLLGV
                     FEFSRVLTGFGLELVVYPAVYSLGRWASVGRNPLGDVGAPSRTRAGESVDFHGIREYR
                     SGDEPRRIHWPTTARTGNLSVIEFEDLPSRGMVAIVDCGAGTNIGPRGSSTLDCAARA
                     AASLAALACGWHSPFELVAVDSMNHGVSIPAGSMSAHRVFEALARLEGRGESPIDQVC
                     AQVARRVDRGASVFVFTSDCGPAAARAALCLLAAGLEATVVLLVPAADGADGVGKPPP
                     LWERLRRRATRSERGGSVEPEGLGLLEALNRSAARIVTVDCGAPLGPQLEALLNG"
     gene            991..>1737
                     /locus_tag="PLQ54_17730"
     CDS             991..>1737
                     /locus_tag="PLQ54_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95160.1"
                     /translation="MASSARAADQLAVQADAWRASRTFLLPWYASALAAMWCVALGVA
                     STAASPGFAPLVLALVTAGVVWGYVLVRAPIADGSRALASAVPVGVVLVAMQTPAIHS
                     VLFSADVVVDADLVLCVRVLTGLLALSFASAYRPVVLFMFVFGLSALGLVGELNVNVE
                     AMACFLAFVYCSLFFLGYDNLLRRGRLSALTAAGGLRRVVGDQSYAAAAVFVVLALSG
                     YAVGTVLTFGLRSPFAWPIHQLSKTVENAPE"
BASE COUNT          194 a          528 c          684 g          331 t
ORIGIN      
        1 tggccgagga tggcgaactg ggaacgctgc tgggcgccga gacgcgcgag atcgaggtta
       61 ccttccacac gctggcccgg ggcgtctatc gggttgacga ggcggaactg gagaccaccg
      121 acctgctggg cgtgttcgag ttctcgcggg tcctcacggg cttcgggctt gagttggtgg
      181 tgtacccggc cgtctacagc ctgggccgtt gggcgtcggt ggggcggaac ccgctggggg
      241 atgttggggc cccgagccgc acgcgggccg gggagagtgt ggatttccac gggatccgcg
      301 agtaccggtc tggcgatgag cccagacgga tccactggcc gacaacggcg cgcacgggga
      361 acctgtcggt gatcgagttc gaggatctgc catccagggg catggtggcc attgtggact
      421 gtggtgcggg gaccaacatc ggcccccggg gctccagcac gctggactgc gcggctcggg
      481 cggcggcatc actggcggcg ctggcgtgcg gctggcactc acccttcgaa ctggtggccg
      541 tggactcgat gaaccatggg gtatccatcc ccgccggctc gatgagcgcc catcgggtgt
      601 tcgaggccct ggcccggctc gagggccggg gcgagtcgcc catcgatcag gtgtgtgccc
      661 aggtggcgcg ccgagtcgac cgcggggcct cggtgttcgt gttcacctcg gactgcgggc
      721 ctgcggcggc tcgagcggcg ctctgcctgc tggcggcggg cctggaggcg accgtggtgc
      781 tcctggttcc ggccgcggac ggcgctgatg gcgtggggaa gccgccgccg ctgtgggagc
      841 ggctgaggcg gagagccact cggtcggagc gcgggggttc ggtcgagccc gaggggctcg
      901 ggctcctgga ggcgctgaat cggagcgcgg ctcgcatcgt gaccgtcgac tgcggcgcgc
      961 cgctggggcc gcagctggag gcgctcctga atggctagct cagctcgagc ggccgaccag
     1021 ctcgccgtcc aggccgacgc atggcgggca tcgcgcacat tcctgctgcc ctggtacgcg
     1081 agtgcgttgg ccgccatgtg gtgtgtggca ctgggcgtgg cgtcgacggc tgccagcccg
     1141 gggttcgcgc ccctggtgtt ggcgctggtg actgccggag tcgtctgggg ttacgtgctg
     1201 gtgcgggcgc ccatcgcgga tggcagtcgg gctctggcgt cggccgtgcc ggtcggcgtg
     1261 gttctggttg ccatgcagac tccggccatt cactcggtgc tgttctcggc cgatgtcgtt
     1321 gttgacgccg atctggtcct gtgtgtccgg gtactgacgg gcctgctggc gctgagcttc
     1381 gcctccgcgt accgtcctgt ggttctgttc atgttcgtgt ttgggctgag cgcgctgggc
     1441 ctggtgggcg agctgaacgt gaacgtggag gccatggcct gcttcctggc gttcgtctac
     1501 tgcagcctgt tcttcctggg gtatgacaac cttctccggc gcggccggct gagcgccctg
     1561 acggccgcgg gcgggttacg acgtgtcgtg ggcgatcagt cctatgcggc cgcggcggtg
     1621 ttcgtcgtgc tggcgctgag tggctatgcc gtgggcacgg tgctcacctt tgggttgcgg
     1681 tcgcccttcg catggccgat ccaccagttg tccaagacgg tggagaacgc gccggag
//
LOCUS       DAOSPL010001157         1737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_69010_length_1737_cov_3.167658, whole genome shotgun sequence.
ACCESSION   DAOSPL010001157 DAOSPL010000000
VERSION     DAOSPL010001157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69010_length_1737_cov_3.167658"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1723)
                     /locus_tag="PLQ54_17735"
     CDS             complement(<1..1723)
                     /locus_tag="PLQ54_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95161.1"
                     /translation="MLLRTRLSAHVFEPLTIPSVALVPFALAGALLSYAFHRHAAWSG
                     RLYFADLSGAAVGSLGVILALNAVGGTNVPFVAGALALLAAVILAAASHQRGLTGAAI
                     LCLVGVVALLGANIDGRIIDLPVLTSAQSQRPHVKPLFRELGDPSVGAKIVATEWNAF
                     ARTDAVANAGTDVLFVYTDGDVPTNMVPFDGDLTPQDVSMLRTFIGFLAFASGPRDEV
                     LLIGPGAGLDVLFGLMAGSRHMVGVELNPSIPRMMYDLRDFNGSLYAYDNVEMHIAEG
                     RSFVASDRRRYDLIYTALTKTATTSTSGLALVEGYVYTKEAFADYLDHLTDSGRIGLV
                     TDNPVLAARALLTVVSVLADRGIPVQDGCQHVALLQIGPQWGADTSSNPYRCLLLARR
                     TPFPESDRDALMTAARILKLEPLFVPGRSEGYLYLDRISQRGDTIDSLVAASTDYNLS
                     PVTDDRPFFIDLSRTWPPELRALATLCVGALVAALWLGTVMLVLGPCRPARTRPWLAS
                     HALGAAVALCMPAPWLFRLGVAATASVAVGALALRQAPAEARDGARRLGALTAYFVVL
                     GIGFLAIE"
BASE COUNT          330 a          576 c          605 g          226 t
ORIGIN      
        1 cctcgatggc caggaacccg atgccgagca caacgaagta ggccgtcagg gctcccaggc
       61 ggcgggcgcc gtctcgggcc tccgcggggg cttgccgcag tgccagagcg cccacggcca
      121 cggatgcggt ggccgccaca ccgagccgga acagccacgg tgccggcata cacaaggcga
      181 ccgccgcccc caaggcgtgg ctggcgagcc acgggcgggt ccgtgccggc cggcagggcc
      241 cgagtaccag catcaccgtg cccagccata gcgccgccac caaggcaccc acgcagagtg
      301 tggccaatgc tcgaagctcg ggtggccacg ttcgggacag gtcgatgaag aacggtcggt
      361 catcggtcac gggcgacagg ttgtagtcag tggatgcggc caccaacgaa tcgatggtgt
      421 ccccgcgctg cgatatgcga tcgaggtaca ggtaaccctc ggagcggccg gggacgaaca
      481 gcggctcgag cttgaggatg cgcgcggccg tcatcagcgc atcgcggtcg ctctctggga
      541 acggcgtccg ccgggccaga agcaggcagc ggtacgggtt gctgctggtg tcggcgcccc
      601 actgggggcc gatctggagg agggccacgt gctggcaccc gtcctgcacc ggaatgccgc
      661 gatccgccag cacggagacg acggtgagca gcgcgcgagc ggccagaacc gggttgtccg
      721 tgaccagccc gatccggccc gagtcggtca ggtggtcgag gtagtccgcg aacgcctcct
      781 tggtgtaaac atagccctcc accaaggcga gacccgaggt ggatgtggtg gccgtcttcg
      841 tcagcgccgt gtagatcagg tcgtatcggc gccggtcgct ggcgacgaag ctgcggcctt
      901 cggcgatgtg catctcgacg ttatcgtagg catagagtga cccgttgaag tcgcgcaaat
      961 cgtacatcat ccgcgggatc gacgggttca gctccacgcc gaccatgtgg cgcgagcctg
     1021 ccatcagccc gaagagcacg tcgaggccag ccccgggacc aatcagcaac acctcgtccc
     1081 ggggtccaga ggcgaaggcg aggaagccaa tgaaggtccg cagcatcgac acatcctggg
     1141 gcgtgagatc cccgtcgaac ggcaccatgt tcgtgggcac gtcaccatcg gtgtagacga
     1201 acagcacgtc agtgccggcg ttcgcgaccg cgtcggtgcg cgcgaaagcg ttccactcgg
     1261 tcgcgacgat cttcgccccg accgacgggt cgcccagctc acggaacagc ggtttgacat
     1321 gcgggcgctg tgactgggca gaggtcagca cgggcaggtc gatgatccgc ccgtcgatgt
     1381 tggccccaag cagggccacg acgcccacca gacagaggat ggcggcaccc gtcagtcccc
     1441 gctggtgcga agccgcggcg aggatgaccg ccgccaggag cgccaaggct cccgccacaa
     1501 agggcacatt ggtgccgccg accgcgttga gggccaggat gaccccgagc gaccccacgg
     1561 ccgcccccga caggtccgcg aagtagagcc gaccactcca cgcggcgtgc cgatgaaagg
     1621 cgtaggacag aagtgcgccg gccagagcga acggcaccag cgccaccgag gggatcgtca
     1681 gtggctcgaa cacatgggcg gacagtcgtg ttcgaagcag caccaccacc gacagag
//
LOCUS       DAOSPL010001158         1736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_69067_length_1736_cov_2.920880, whole genome shotgun sequence.
ACCESSION   DAOSPL010001158 DAOSPL010000000
VERSION     DAOSPL010001158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69067_length_1736_cov_2.920880"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="PLQ54_17740"
     CDS             <1..952
                     /locus_tag="PLQ54_17740"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOS95162.1"
                     /translation="RSLATLTAMGALALGCCQAWWLLRRVRPAAVFGTGGYASACVVY
                     AAAHLGIPTLIHDLDASPGRASRRLARLATRVTVGYPEAAAALGRPDAIHTGNPIRGE
                     IGSASRAEGLAAFGLAADRRTLLVLGGSQGARRLNDAVLTGASALVCELALQILHITG
                     PADWDRVRAGREALPPDVLAHYQVRPYLEGTMHLALAAADVCLCRSGAGTVGELTAVG
                     IPGIYVPYPFALGHQAKNAEAVAQSGGGTIIQDGVLTGESLVAEIRALLEGDRLASMA
                     AAARACGRPDAADHIAAVLLDMVGEGCEHGRSGGAGRPLG"
     gene            915..>1736
                     /locus_tag="PLQ54_17745"
     CDS             915..>1736
                     /locus_tag="PLQ54_17745"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOS95163.1"
                     /translation="MGDRVVRDGRWDNLDLDQVHLIGIGGVGMSGLARVLLDRGMAVS
                     GSDASDSATVRELRQLGANVFVGHSAAHIGETVDTVVVSTAVRDDNPEVLEARRRSLR
                     LWHRSELLAYTVRGYDMLAVAGTHGKTTTTGLLGHILECSGLDPLVLLGGSSVAWGSN
                     TRLGNGRYAVVESCESDRSFVNYRPAAAIVTNVEAEHLDVYAGLTDVVDAFGAFVALV
                     SPNGCVALGADCPNALSLRDAAPCPVVTFGFDPSADFRVSGFRMEQDRSAFHLSGP"
BASE COUNT          240 a          556 c          637 g          303 t
ORIGIN      
        1 caggtcgctg gccaccctca cggccatggg cgccctggca ctcggctgtt gccaagcatg
       61 gtggttgctg cggcgcgtcc gcccggcggc ggtctttggt acgggtggct acgccagtgc
      121 gtgcgtggtc tatgccgccg cacatctggg catcccgacc ctgattcatg acctggacgc
      181 gtcgccgggt cgcgcgagcc ggcggctcgc gcgactggcc acccgggtca ccgtgggcta
      241 ccccgaggcg gccgcggcct tgggacgccc cgacgccatc cataccggca acccgatccg
      301 cggtgagatc gggtcggctt cgcgggccga gggcctggcg gccttcggtc ttgcggctga
      361 tcgccgaacg ctcttggtgc tggggggcag ccagggggca cggcggctga acgacgccgt
      421 gctcaccggg gcgagcgctt tggtctgcga gctggccctc cagattctgc acatcaccgg
      481 accagcggat tgggaccggg tccgcgcggg aagggaggcc ctgccgcctg atgtcctggc
      541 ccactaccaa gtcaggccct acctggaggg caccatgcac ctggccctgg cggcggccga
      601 cgtctgcctg tgccgcagcg gggcgggaac cgtcggcgag ctgaccgcag tgggcatacc
      661 gggaatctac gtgccctatc ccttcgccct cggtcaccag gccaagaacg ccgaggccgt
      721 ggcccaatcc ggaggaggta cgatcatcca ggacggcgta cttacgggag agtcgttggt
      781 cgccgagatc cgggctctcc tggagggaga ccggctggca tcgatggccg cggccgctcg
      841 ggcatgtggc cgcccagacg ccgcagatca catcgcggcc gtgttgcttg atatggtggg
      901 ggaaggttgt gagcatggga gatcgggtgg tgcgggacgg ccgctgggat aacctggacc
      961 tcgaccaggt ccatctgatc ggcatcggtg gcgtcgggat gagcggtctg gcgcgcgtgc
     1021 tgctcgatcg gggcatggcc gtctcgggat ccgatgcatc ggactcggca acggtccggg
     1081 agctgaggca gcttggtgcg aacgtgttcg tcggacactc ggcggcacac atcggcgaga
     1141 cggtggacac ggtggtggtg tcgaccgcgg tccgagacga caaccccgag gtgctggagg
     1201 ctcggcgccg cagtctgcgt ctctggcatc gttccgagct gctggcgtac acggtgcgcg
     1261 gctatgacat gctcgccgta gcaggcacgc acgggaagac caccaccaca ggcctccttg
     1321 ggcacatcct ggagtgcagc ggccttgacc cgctcgtgct cctgggtgga agtagcgtcg
     1381 cctggggttc gaacacccgg ttggggaacg ggcgctacgc cgtagtggag tcgtgcgaga
     1441 gcgaccggtc gttcgtgaac taccgccctg ccgcggccat cgtcaccaat gtcgaggccg
     1501 agcatctgga cgtctacgcc ggtctgacgg atgtggtgga tgcctttggc gcgtttgtgg
     1561 cactggtctc gccgaacggg tgcgtggcgc tgggtgctga ctgccccaat gccctctcgc
     1621 tgcgcgatgc cgcgccctgt ccggtggtca ccttcgggtt cgacccgtca gccgactttc
     1681 gtgtgagcgg gttccggatg gagcaagacc ggtcggcctt ccatttgtcg gggccg
//
LOCUS       DAOSPL010001159         1734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_69170_length_1734_cov_3.114354, whole genome shotgun sequence.
ACCESSION   DAOSPL010001159 DAOSPL010000000
VERSION     DAOSPL010001159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69170_length_1734_cov_3.114354"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1308
                     /locus_tag="PLQ54_17750"
     CDS             124..1308
                     /locus_tag="PLQ54_17750"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010044681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOS95164.1"
                     /translation="MFGRMRDHLTEELGRIRESGLYKNEWIIDTPQGARVRLEDGAPL
                     LNLCANNYLGLASHPEVIAAAHRALDRWGYGMASVRFICGTQRIHKALEAKISEFLST
                     DDTILYSSCFDANGGLFEALLGPDDAIISDALNHASIIDGVRLCKARRLRYANNDMAE
                     LESALKESADARFRLIATDGVFSMDGTIANLRAICDLADQYDALVMVDDSHAVGFMGP
                     AGRGTHEYRDVMGRVDIITGTLGKALGGASGGYTSGRAEIIEMLRQRSRPYLFSNTVA
                     PPIAATSLAALDLLSRSSELRERLHANTRLFRHGMAARGFDIRPSEHPIVPIMLGDAA
                     LAVRMAKAMLERGVYVVAFSYPVVPEGAARVRVQVSAVHTPDDLEFAMDAFAEARRAL
                     GG"
     gene            complement(1372..>1734)
                     /locus_tag="PLQ54_17755"
     CDS             complement(1372..>1734)
                     /locus_tag="PLQ54_17755"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS95165.1"
                     /translation="RCAAIVADLPDTGADCVCPFERPPGGDTTDLSVVREQLRDRVTV
                     NGNVHTVETLIRGGPDAVSLQVEEILDRWGVPPSRLILGTGDQVGRETPEENIAAMIE
                     TGRRLGRAAAGRAWGHPG"
BASE COUNT          270 a          628 c          552 g          284 t
ORIGIN      
        1 acgtgctgcc ctatgaccgc ttcgaggaag gcttcgaggc catgcgctcc ggtcgatccg
       61 gcaaggtcgt gcttgtgtgg gacgatgaag ggctgcccga gtagggcccg ggagggcgac
      121 ggcatgttcg gacggatgag agatcatctg acggaggagc ttgggcggat ccgcgaatcg
      181 gggctctaca agaacgagtg gatcatcgac accccccagg gggctcgtgt ccgcctcgaa
      241 gatggcgccc cgctcctgaa cctgtgtgcc aacaactacc tcggtctggc gagtcatccc
      301 gaggtcatcg ctgccgccca ccgggcgctc gaccgctggg gctacgggat ggcctccgtt
      361 cggttcatct gcggcaccca gcggatccac aaggccctcg aggccaagat cagcgagttc
      421 ctctccactg acgataccat cctctactcg tcttgctttg atgccaacgg cgggctgttt
      481 gaggcactgc tcgggcccga cgacgcgatt atctcggacg ccctgaacca cgcgagcatc
      541 atcgacggcg tccggctgtg taaggctcgt cggctccgct atgccaacaa cgacatggcc
      601 gagctcgaat cggcactcaa ggagtctgcc gacgcccgct tccggctcat cgccaccgac
      661 ggcgtgttct ccatggacgg caccatcgcg aacctccgag ccatctgcga cctggccgac
      721 cagtacgacg ccctcgtcat ggtcgatgac tcccatgccg tcggattcat gggcccggcg
      781 ggccgtggca cccacgagta ccgcgatgtc atggggcgag tcgacatcat caccggcact
      841 ctgggcaaag cgctgggcgg cgccagcggg ggatacacca gcggccgggc ggagatcatc
      901 gagatgctgc gccagcgctc ccggccctat ctcttctcga acaccgtcgc accgcccatt
      961 gccgccacct cgctggcggc cctcgacctg ctgtcccgct cctccgagct ccgcgaacgc
     1021 ctgcacgcca acacccggct gttccgccac ggcatggcgg cccgcggctt cgacatccgc
     1081 cccagcgagc accccatcgt gcccatcatg ctcggggatg cggccctggc cgtccgcatg
     1141 gccaaagcca tgctcgagcg cggcgtgtac gtggtcgctt tctcctaccc ggtggtccct
     1201 gagggcgcgg cccgagtccg ggtccaggtc tccgccgttc acacgcccga cgatctcgag
     1261 tttgcaatgg atgcgttcgc tgaggcgagg cgggcgctcg gcgggtagtc cgggacgagc
     1321 cagagccagc cggggcgtct ccggccgggc cgaacggcgc gggccgcggc ctcaccccgg
     1381 gtggccccat gccctccccg cggccgcgcg accgagccgg cgccccgtct cgatcatcgc
     1441 ggcaatgttc tcctcaggcg tctcccggcc cacctggtca cccgtgccca ggatcagccg
     1501 tgacggcggc actccccatc ggtcgaggat ctcctcgacc tgaagagaca ccgcgtccgg
     1561 gccgccgcgg atgagtgtct ccaccgtgtg cacgttacca ttgacagtca cccggtcacg
     1621 gagctgctcc cgaacgaccg ataggtctgt cgtgtcgccg ccgggcgggc gctcaaatgg
     1681 gcacacgcag tcggcacctg tgtccgggag atcggccacg atggccgcgc accg
//
LOCUS       DAOSPL010001160         1734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_69177_length_1734_cov_2.646814, whole genome shotgun sequence.
ACCESSION   DAOSPL010001160 DAOSPL010000000
VERSION     DAOSPL010001160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69177_length_1734_cov_2.646814"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PLQ54_17760"
     CDS             <1..1131
                     /locus_tag="PLQ54_17760"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOS95166.1"
                     /translation="ALLGTEGLAAVGPRSAHIAGLPYYGYGLPAPPLKAVLMAPIAGD
                     PGEYVALEDEAGRRWAFTLTCAARTLRGEVPEDGAAAAGRLVGLGAREMCQAMLERAI
                     EPLSAAVLELASEHGLDGPRLTLVGGGGGAEAIVPHLAERIAVPWSICPDAPVVSAIG
                     AALAMMHETIERSVANPSPADIAAIRQEVRQSLLRHGASDDSVEVRVEFEAAAGVLRA
                     TATGAAALSAGPARAGLADDALAERARAVLRRYGADEVTRVGRSEFLSVYQGEVRTRR
                     ALGLLRRTTHPTCVVDSTGAVRLQRPDGVCITTTAQEAARALASEARYGDAGLALPPA
                     RVISAARIVDVSGLATLEQASALLEAELAGVAAESPVLIVVG"
     gene            1231..>1734
                     /locus_tag="PLQ54_17765"
     CDS             1231..>1734
                     /locus_tag="PLQ54_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95167.1"
                     /translation="MVFLPTAAFGNSRFLMTVGRSGEVMGLFYPHIDHAQNVRECLFG
                     VYFADSPEGRFAWSWDDMWRIEQGFSNNGDTEFASSILRTVLSNPRTGLVVELVDLVP
                     ADAPAWVRQVSVSGGHGRLKVCQYFQLNLGDVARGNAVHVYPDERQCIQRYRETTFCI
                     TADRGFEH"
BASE COUNT          256 a          577 c          609 g          292 t
ORIGIN      
        1 gcgctcctgg gcaccgaagg cctcgctgcg gtggggcctc ggagcgccca tatcgcgggc
       61 ttgccctact acggctacgg gttgcccgcc ccgccgctca aggcggtcct catggcaccc
      121 atcgccggcg accccggtga gtacgtggct ctcgaggacg aagccggtcg gcggtgggct
      181 ttcacactga cctgcgccgc gagaacactg cgaggcgagg tcccagagga cggggccgcc
      241 gccgccgggc gtctggtcgg gttgggcgcc cgcgagatgt gccaggccat gctggaacgc
      301 gccatcgagc cactatccgc agccgtgctc gaactggcct cggaacatgg gctcgacggt
      361 ccccggctca cactggtggg aggcgggggt ggagcggagg ccattgtgcc tcacctggcg
      421 gaacgaatcg cggtgccctg gagcatctgt ccggatgcgc cggtggtttc cgccatcggc
      481 gcggcgctcg ccatgatgca cgagacgatc gaaaggagcg tggcgaatcc ctcccccgcg
      541 gacatcgcgg cgatacgcca ggaggtccgg cagagcctgc tccgccatgg ggccagcgat
      601 gacagcgtcg aagtgcgggt cgagttcgag gcggctgccg gcgtcctgcg cgccactgcc
      661 acgggagcgg cggcgctctc cgccggcccg gcccgggccg ggctggctga cgacgccctg
      721 gccgaacggg cgagggccgt tctgcgccgc tacggtgccg atgaggtcac gcgagtgggc
      781 cgcagtgagt ttctctccgt ataccagggg gaggtgcgaa cgcgccgggc tctggggctg
      841 ctgcgccgga ccacacaccc tacgtgtgtc gtggactcca cgggcgcagt tcgactgcag
      901 cgccccgacg gtgtctgcat caccacaacg gctcaggagg ccgctcgcgc cctggcgtca
      961 gaggcacggt atggcgatgc aggtctcgcg ctacccccgg cacgggtcat ctcggccgcg
     1021 cgcatcgtcg acgtgtccgg cctggccact ctcgaacagg cttcggcgct cctggaggcc
     1081 gagctcgcgg gcgtcgccgc cgaatcgccc gtgctgattg tggtgggata ggcctttcgg
     1141 ggattgggtc tgcatggaat gatgatcctt gtggcctgag catcccggtc gacaactggg
     1201 gtcagaccac tgagagggtg ctcgccgtct atggtgtttc tcccgacagc cgcatttggc
     1261 aactcacggt ttctgatgac cgtcggacgc tccggcgagg tcatggggct gttctacccg
     1321 cacatcgatc acgcgcagaa tgtgcgcgag tgcctctttg gcgtctactt cgccgattcc
     1381 cccgagggac gcttcgcctg gtcgtgggac gacatgtggc ggatcgaaca ggggttctcg
     1441 aacaatggcg acacggagtt cgcctccagc atactccgca cggtgctatc gaacccgcgg
     1501 actggcctcg tcgtcgagct cgtggacctc gtgcccgcgg acgcccctgc ctgggtgcgc
     1561 caggtgtcag tctcgggcgg acacggccga ctcaaggtgt gtcagtactt tcagctaaac
     1621 ttgggcgacg tggcccgagg caacgccgtc cacgtatacc ctgacgagcg ccagtgcatc
     1681 cagcgctacc gggagacaac gttctgcatc acggcggacc gcgggttcga gcac
//
LOCUS       DAOSPL010001161         1734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_69185_length_1734_cov_2.434187, whole genome shotgun sequence.
ACCESSION   DAOSPL010001161 DAOSPL010000000
VERSION     DAOSPL010001161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69185_length_1734_cov_2.434187"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PLQ54_17770"
     CDS             complement(<1..277)
                     /locus_tag="PLQ54_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95168.1"
                     /translation="MVVVGVAALAVVALMARPVAGQFGGWQPQGGPAGPSPAGPMPGT
                     MPGMGPMAPAAAIAVADGKVYVVLGATVYKLDADSLEIEKQHALVRPP"
     gene            449..1153
                     /locus_tag="PLQ54_17775"
     CDS             449..1153
                     /locus_tag="PLQ54_17775"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOS95169.1"
                     /translation="MLTLHSSLLLAMVPVVAVAAEIPSEAPNDAITAPESDVDELHEW
                     ASSAFLGQAPELRGARCRLRVRRQDHSVLRFRQSCIDTPICVGTRTYDHGLGTHAQSE
                     IAVEIPHGAEVFEAAVGVDNNADTGGTHGTVEFVVRVDGAEAYRSPVCCGGGEPVPVR
                     VELPAGSRELMLIALPTDDGAGYDQADWADAAFIMADGTRRWLDDNQIDLLLAEAGLP
                     FSFTYGGRPSGELLAA"
     gene            1190..1666
                     /locus_tag="PLQ54_17780"
     CDS             1190..1666
                     /locus_tag="PLQ54_17780"
                     /inference="COORDINATES: protein motif:HMM:NF028185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 N-terminal
                     domain-containing protein"
                     /protein_id="HOS95170.1"
                     /translation="MFHTVRWRDPETGLTVTAEVKTFRQTPAAEWVLYFENQGDADTP
                     IIEGIQALDVSLRTGYGKRPTQIHQLRGDSCGELSFLPFSTDLEAGRTYAMAPTGGRP
                     SSISAFPFFNVQYDRGGAIVAVGWSGQWAATFERAANGPTRLAAGMERTHLLLHPG"
BASE COUNT          270 a          609 c          572 g          283 t
ORIGIN      
        1 gcggcgggcg cacaagcgcg tgctgcttct cgatctccag cgaatcggcg tcgagcttgt
       61 agaccgtcgc gcccagcacc acatagactt tcccgtcggc cacggcgatg gcagccgccg
      121 gagccatcgg ccccatgccg ggcatggtgc ccggcatggg gcctgcggga ctgggcccgg
      181 ctggaccgcc ctggggctgc caaccaccga actggccagc caccggccgc gccatcagtg
      241 ccacgactgc caacgccgcc actccaacca cgaccaccca ccgccggctc ctgctcgaag
      301 aggtgctgtg ttctctcatg tgacgtaccc cctctgaccc tttggcgatt ccataggctg
      361 catcagcggg ataggccctg ccctcgggag gcttcgccgc ggggtgagga gaacctccgc
      421 gcatcacttg gacggacgga gaacgcgtat gctgaccctc cacagctcgc tgttgctcgc
      481 catggtgccc gtggtcgccg tcgcggcgga gatcccttca gaggccccca acgacgccat
      541 cactgccccc gaatccgatg ttgatgagtt gcacgagtgg gcctcaagtg cgttcctcgg
      601 gcaagcgccc gagctgcgcg gcgctcgatg ccgactccgc gtgcggcggc aggaccacag
      661 tgtgttgcgc ttccggcagt cctgcatcga cacaccaatc tgtgtgggta cccggaccta
      721 cgaccatggc ctggggaccc acgcgcagag cgagatcgcg gttgagattc cccacggtgc
      781 cgaagtcttc gaggcggctg tgggtgtgga caacaacgcg gatactggcg gcacgcacgg
      841 cacggtcgag ttcgtggtgc gggtcgatgg agccgaggcc taccggtctc ccgtgtgctg
      901 cggtggcggc gaaccggtgc ctgtgcgagt cgagctcccg gcgggcagcc gtgaactcat
      961 gctgatcgcc ctgcccaccg acgacggggc agggtacgac caggccgact gggccgacgc
     1021 cgccttcatc atggccgacg gtacccgccg ctggctcgac gataaccaaa tcgacctgct
     1081 tctggccgag gctgggctgc cgttctcgtt cacctacgga ggcaggccat cgggcgaact
     1141 tctggcggcc taggagcgca cgtctagctc ggaggagcgc gccgatcacg tgttccatac
     1201 ggtccgctgg cgcgaccctg agaccgggct gaccgtcacc gccgaggtga agacgttcag
     1261 gcagactccg gcggcggagt gggtcctcta cttcgagaac caaggcgatg ccgatacgcc
     1321 catcatcgag ggcatccagg ccctcgatgt gagcctgcgc actggctacg gcaagcggcc
     1381 cacgcagatc caccagctcc gaggcgactc gtgcggcgag ctctccttcc tgcctttcag
     1441 cacggacctg gaggcgggcc ggacctacgc catggccccg acgggcgggc ggccctcgtc
     1501 gatcagtgcg ttcccgttct tcaacgtgca gtatgaccgt ggcggcgcca tcgtcgccgt
     1561 gggctggtcc ggccagtggg ccgccacttt cgagcgcgcc gcgaatgggc ccacccggct
     1621 cgcggccggc atggagcgga ctcatctgct gctccatccc ggttagcgta tccgcacccc
     1681 gcgggtgctg gtcatgccct ggaagggcga ccgcatggcc gcccacatac ggtt
//
LOCUS       DAOSPL010001162         1732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_69278_length_1732_cov_3.192010, whole genome shotgun sequence.
ACCESSION   DAOSPL010001162 DAOSPL010000000
VERSION     DAOSPL010001162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69278_length_1732_cov_3.192010"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(412..894)
                     /locus_tag="PLQ54_17785"
     CDS             complement(412..894)
                     /locus_tag="PLQ54_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95171.1"
                     /translation="MRHENVRARQSPYNRTVFDISMANPSINPYMPQPPHVQAAIDDY
                     LAGRSDSPYLPGSGLYGPQRHEQAGIEPYRSNPWSDPHLMRHSYEQTSPGPWQAPVAV
                     PPASAHCPPSLLQHYLPIPPPSQPAADRFWDQHGGTLVGGLIFGAGVWLASLVLRRPL
                     "
     gene            1119..>1732
                     /locus_tag="PLQ54_17790"
     CDS             1119..>1732
                     /locus_tag="PLQ54_17790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95172.1"
                     /translation="MTSSLLFIDESGNERVEADWLVVAGVLVEPQDRDSFQGAVREAL
                     GRLMPGLGYPPHASVQGSPAGRLAFTWLNERDAAAQSLELRALLEETRRLSSCPLPRR
                     VQAFFDALARGREPDYVTEMGPCNDWLRSSRPRLHEQWQQFVDRDREGMVRLLQRLQE
                     SSSLAVVAAAAQVGDLNFDEPGPSGFAYGLYLTLLEVLLERAVAL"
BASE COUNT          254 a          575 c          624 g          279 t
ORIGIN      
        1 cgctggccgc caagccctcg taccccggcc cgggcacctg gctgcacggc ggcgtactcg
       61 cggcgcgctt gcactgcacg tccttcgcga cgggacgacg aggctcgacc ttcaccgccc
      121 cgcactctcg gttcacgagg tcgaccgcga gccggccccc cgcgtggccc gccaccgcgc
      181 ggaaccagcg cgagccgacc agggcgggac ggcaccggtg actgcgccgg cggcggtgaa
      241 gcagacgacg gtgttccggg cttgggagag ggtttcgtcg gtgatcatgc caggtcgacg
      301 ttcggacgcg aaggagctta caacacgggc gctggatcgc ggcgagagtg ggtcggataa
      361 gaggggaggg atcgtgtcca ggtccggtag cggaagaacc gccgcgggcg gctacagcgg
      421 cctcctcaga accaaggagg cgagccacac tcccgcgccg aagatcagcc cgccgaccag
      481 cgtgccgccg tgctggtccc agaagcggtc ggcggcgggc tgcgacggtg gcgggatggg
      541 gaggtagtgc tggagcaagg acggcggaca gtgagccgat gccggcggca cagcgaccgg
      601 cgcctgccat gggccaggag aagtctgctc gtaggaatgt cgcatcaggt gcgggtcgga
      661 ccacgggttc gaccggtacg gctcgatacc cgcctgctca tggcgctgcg gcccgtacaa
      721 gccgctgccc ggaaggtatg ggctgtcgct ccgaccggcc agatagtcgt cgatggcagc
      781 ctgcacgtgc ggtggttgtg gcatgtaggg gttgatgctc ggattcgcca tggagatatc
      841 gaacacggtc cggttgtacg ggctctgcct ggcgcgaacg ttttcgtgcc tcatgcctcc
      901 cagacgcccg tgccctgacg atctgacttc gtccgccgcc ctcgccccgc gccgcgcgga
      961 gggctcgcta cgcgacggcc ggcttggccg cgggggagag tcacgacgtt cgcgcccgga
     1021 tcgcgcaggc gatggacgag cgggaggaag cgccggggcg tcgagccccg cttaccgcct
     1081 agtctgacgc tgccgggacg ggtacaatcg gcgctgtcgt gacaagctcc ctgctcttca
     1141 tcgacgagtc cgggaacgag cgtgtcgagg cggactggct ggtggttgcc ggggttctcg
     1201 tcgagcccca ggaccgtgat tcatttcagg gcgcggtgcg cgaggcgctg gggcgcctga
     1261 tgcccggact tggctacccg ccgcacgcca gcgttcaggg atccccagcg ggacgcctcg
     1321 cgttcacctg gctgaatgag cgcgacgccg ccgcccaatc cctggagctt cgcgcactct
     1381 tggaagagac gaggcgtcta tcgtcctgtc ccctaccgcg ccgcgtgcag gccttcttcg
     1441 atgctctggc gaggggccgc gagccggact acgtcacgga aatggggccg tgcaacgact
     1501 ggctccggtc ttcccggccc cgccttcatg agcagtggca gcagttcgtg gaccgtgacc
     1561 gggaagggat ggtccgcctg ctccagcggc ttcaggagtc gagctctctc gccgtcgtcg
     1621 ccgcggccgc ccaagtgggg gatctcaact tcgacgaacc cggcccctcc ggtttcgcgt
     1681 atgggctcta tttgaccttg ctcgaggttc tcctcgagcg cgcggttgcc ct
//
LOCUS       DAOSPL010001163         1730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_69388_length_1730_cov_3.088955, whole genome shotgun sequence.
ACCESSION   DAOSPL010001163 DAOSPL010000000
VERSION     DAOSPL010001163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69388_length_1730_cov_3.088955"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1730)
                     /locus_tag="PLQ54_17795"
     CDS             complement(<1..>1730)
                     /locus_tag="PLQ54_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95173.1"
                     /translation="WGGANDGEFHFPLTTILIGVNRGPTLQGSCLIDDLTFRTSAPEL
                     RYRLTLRTGEPGDVVFASGDRVPVAVTVENAVDEEARLALRVVAESWHGDRVELLDRD
                     LLLPAFAAATQTVTLPGSSPEYWALTARVARGDDVLARGLGAVVVVERPRNFGQDDPS
                     SFFGIQCSSNGARTERLGAKWVRAGRDWKWAEMQRGSYWLPDLSEIRANHQLIMYTMT
                     AYPPAWAEEQAAGHDFWVGDRAAERIAWWAAFVEHSARELAPMVDTFEIQNEPDLTCM
                     WQVGLDLAKGAERYARILESAAAAVRRGAPGARVAGIDVSGGDYDGGLPFSEAMMASC
                     GNLIDVYTGHPYAGVRYFGEGQQPMWPVRNEERRKCLDTIAMIDRHGGSQRFWIGEKG
                     WGLDVSAPSLSDHSRQFAECLVQSMVIAHSVPRVERYFWFLEEGCNEGGYEYGLFRKG
                     MPLPAALAYSTLASKLHHATPVDSPVLSPNLQAHLFASEDTGQATLVAWSEDGAANLA
                     IARAPAEWRVDDLMGRPLADGGRGEAIRVALDRAPTYIQVPLDALSDFRQALTEAAVS
                     VAVPVRIEAA"
BASE COUNT          279 a          566 c          618 g          267 t
ORIGIN      
        1 ggcggcttcg atccgcaccg ggacggcgac ggacactgct gcctcggtca gcgcttgtcg
       61 gaagtcggac agagcgtcga gggggacctg aatgtaggtg ggcgcgcggt cgagcgcaac
      121 gcggatggct tcgcctcgcc cgccgtcggc caatgggcgg cccatcaggt catcgacgcg
      181 ccactcggcc ggcgcgcggg cgattgccag gttcgcggca ccgtcctcgg accaggccac
      241 cagggtggcc tgcccggtat cctcggacgc gaacaggtgg gcctggaggt ttgggctgag
      301 gacgggcgag tccacgggcg tggcgtggtg caatttgctc gcgagcgtac tgtaggccag
      361 ggcggcaggc agaggcatgc cctttcggaa gagcccatac tcgtagcctc cctcgttgca
      421 gccctcctcc aggaaccaga agtagcgctc gacacgcggg accgagtgag cgatcaccat
      481 tgactgcacc aggcactcgg cgaactggcg ggagtggtcg gacagcgacg gggcggacac
      541 gtcgaggccc cagcccttct cgccgatcca gaagcgctgg ctgccaccgt ggcggtcgat
      601 catggcgatg gtatcgaggc acttgcggcg ttcttcgttg cggacgggcc acatgggctg
      661 ctggccctcg ccaaagtagc ggacgccggc ataggggtgg ccggtgtaga cgtcgatgag
      721 gttgccgcat gaggccatca tcgcctcact aaagggtagc ccgccgtcgt agtcgccgcc
      781 gctgacgtcg atgccggcca cccgcgcgcc gggtgcccct cgccggacgg ccgcggccgc
      841 agactcgagg atgcgggcgt agcgttcagc gcccttggcg agatcgaggc ccacctgcca
      901 catgcacgtg aggtccggct cgttctggat ctcaaaggtg tcgaccatcg gtgccagctc
      961 gcgggcggag tgttcgacga atgcggccca ccaggcgatg cgctcggcgg cgcggtcacc
     1021 gacccagaag tcgtggcccg ctgcttgctc ctcggcccac gcgggcggat aggccgtcat
     1081 cgtgtacatg atgagttgat ggttggctcg aatctcagag agatcaggga gccagtaact
     1141 gccgcgctgc atctccgccc atttccagtc gcggccggcg cgcacccact tggcaccgag
     1201 acgctcggtg cgagccccgt tgctcgagca ctggatgccg aagaagctcg aggggtcgtc
     1261 ctggccgaag ttgcgcgggc gctcaacgac aaccacagcg cccagaccgc gcgcgaggac
     1321 gtcgtcgcca cgcgcgaccc gggctgtgag ggcccagtat tcgggactgg agcccggcaa
     1381 cgtaacggtc tgggtcgccg ccgcgaacgc ggggagcagc aggtcgcggt ccaggagctc
     1441 aacacggtcg ccgtgccagc tctcggcgac cacgcgcagg gcaagcctag cctcctcgtc
     1501 gaccgcgttc tcgaccgtca ccgcgacggg cacccggtca ccggaggcga agaccacatc
     1561 accgggctcg cctgtccgca gtgtgagtcg gtagcggagt tcgggggcgc tggtgcggaa
     1621 cgtgaggtca tcgatcaggc acgacccctg aagtgtgggt ccccggttca cgccgatgag
     1681 gatggttgtc agcgggaagt ggaactcgcc gtcgttggcg ccaccccagt
//
LOCUS       DAOSPL010001164         1729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_69432_length_1729_cov_3.158901, whole genome shotgun sequence.
ACCESSION   DAOSPL010001164 DAOSPL010000000
VERSION     DAOSPL010001164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69432_length_1729_cov_3.158901"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /gene="mdh"
                     /locus_tag="PLQ54_17800"
     CDS             <1..715
                     /gene="mdh"
                     /locus_tag="PLQ54_17800"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOS95174.1"
                     /translation="DLVVITAGLPRKPGMTRDDLLRANADIVSGVVENVMRHSPDPIL
                     LVVSNPLDVMTELAFQRSELPAHRVLGMAGVLDSARFRAFIAMELHVAMEDVHAMVLG
                     GHGDDMVPLPRFSTVAGIPITELIDEGTIERLVDRTRRGGGEIVSLLKTGSAFYAPSA
                     AVYQMVEAIVRDKNRVLPAAARCEGQYGLDDQYVGVPVKLGRRGVVEIIELALTDAEL
                     AALHASAAHVAEQVAALAR"
     gene            752..>1729
                     /locus_tag="PLQ54_17805"
     CDS             752..>1729
                     /locus_tag="PLQ54_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-agarase"
                     /protein_id="HOS95175.1"
                     /translation="MRPHCHIMLTLGFALIALGGGSAMADQVLIDFEGGFDFGAVEAR
                     DVTVSSVEADGSRWLRMDSGHAEPWPGITLVAPGGHWDLSAFAYVLLDVRNLGTRRAS
                     PTIRIDNPGADGQKHCVQVGIDLGPGEKGTIRADLPTVLVDPATGEPIRFFGMRGWPG
                     NMGRREFDPANVTQVLVFHPQPQEDHSIAIDNLRVAGTPQVTQVPSPFFPMIDEFGQF
                     IHRDWPGKTHSERELAERREAEAQELRDRPGPETWDQWGGWQVGPQLEATGWFRVEKV
                     DGQWWLVDPDGRLFWSHGIDCVHYGSEFTPLDDRENWYQWLPGKDSPLGS"
BASE COUNT          278 a          558 c          602 g          291 t
ORIGIN      
        1 ggacctggtc gtcatcactg cggggcttcc acggaagccg gggatgacgc gagacgatct
       61 gctccgcgcg aacgctgaca tcgtcagcgg cgtggtcgag aacgtcatgc ggcactcgcc
      121 ggacccgatc ctgcttgttg tctcgaaccc tcttgatgtc atgaccgaac tggcgtttca
      181 gcgcagcgag ttgcctgccc atcgggtgct gggtatggcg ggcgtgctgg actcggcgcg
      241 cttccgcgcc ttcattgcga tggagctcca cgtggcgatg gaggacgtgc acgccatggt
      301 gctgggcggc cacggcgacg acatggtgcc gctcccgcgt ttctcgacgg tggccggcat
      361 cccgatcacc gagctcatcg atgagggcac tatcgagcgc ctggtggacc gcacgcggag
      421 gggcggcggc gagatcgtga gcctactgaa gaccgggagc gcgttctacg cgccgtcggc
      481 cgcggtgtat cagatggtcg aggcgatcgt gcgcgacaag aaccgcgtgc tgcccgccgc
      541 cgctcggtgc gaggggcagt atggtctcga cgaccagtac gtcggcgtcc ccgtgaagct
      601 cggtcggcgg ggcgttgtcg agatcatcga gcttgccctc actgacgccg agctggccgc
      661 cctccatgca tcggccgccc atgtggccga gcaggttgca gcgctggccc gatgaggggg
      721 tagccccacc cgacgctcag gaggtcgcat catgagacca cattgccaca tcatgctgac
      781 cctcggcttc gctctcatcg cgctaggagg gggctccgcc atggccgacc aggtcctcat
      841 cgacttcgag ggaggcttcg acttcggcgc tgttgaggcc agggatgtca ccgtatcgag
      901 cgtggaagcc gatggctcca gatggctcag aatggacagc ggccacgccg agccttggcc
      961 cggcatcacg ctcgtcgctc cgggaggcca ctgggatctg tcggctttcg cctatgtcct
     1021 cctcgatgtg cgcaacctcg gcacgcggcg cgcaagcccc accatccgta tcgataaccc
     1081 cggggccgac gggcagaagc actgtgtcca ggtcggcatt gacctcggcc ctggggagaa
     1141 aggcacgatc cgggccgatc tgcccacggt gctcgtggat ccggccacgg gcgagccgat
     1201 tcggttcttc ggcatgcgtg gctggcccgg gaacatgggc cggcgggagt ttgatccggc
     1261 gaacgtgacg caggtgctcg tgttccaccc gcagccgcag gaggatcact ccatcgccat
     1321 cgacaacctt cgggtcgccg gcaccccaca ggtgacccaa gtgccgtcgc ccttcttccc
     1381 catgatcgac gagtttggcc agttcatcca ccgcgactgg cccgggaaga cgcactccga
     1441 gcgcgagcta gccgagcgcc gggaggccga agcgcaagag ctgcgggatc ggccggggcc
     1501 ggagacgtgg gaccagtggg ggggctggca agttggcccg cagctcgagg ccaccgggtg
     1561 gttcagagtc gagaaggtgg atggccagtg gtggctggtg gaccccgacg ggcggctgtt
     1621 ctggtcgcac ggcatcgact gcgtccacta cggcagcgag ttcaccccgc tcgatgatcg
     1681 agagaactgg taccagtggc tcccggggaa ggactcaccc ctcggaagc
//
LOCUS       DAOSPL010001165         1726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_69606_length_1726_cov_3.156792, whole genome shotgun sequence.
ACCESSION   DAOSPL010001165 DAOSPL010000000
VERSION     DAOSPL010001165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69606_length_1726_cov_3.156792"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PLQ54_17810"
     CDS             complement(<1..393)
                     /locus_tag="PLQ54_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95176.1"
                     /translation="MLVLWCVAALVRADAGAVDLARGDGARIAAESAQAAVQRPAAAL
                     IDGDPATTFRWEWANGGAEAVIDLGRPALELLGQDAGVDFLKTSTGMHKAGGATAEHV
                     RLMAQTAPRCKIKAAGGKVWSSYFGDLDA"
     gene            846..1604
                     /locus_tag="PLQ54_17815"
     CDS             846..1604
                     /locus_tag="PLQ54_17815"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOS95177.1"
                     /translation="MKPISLQLYSVRDACAVDFPGTCKKVADIGYTGVELAGLHGMTA
                     AQAKAMFDDLGLKVSSQHGPLPTQENVEQLVADCKTFGISQTVTGFGPDDMKTVDATK
                     ACAAKMQAGAELLKGTGIELCYHNHWWEFEPLEGQCAFDILMAEAPDLRAQLDTYWCT
                     FGGADAAAVLKRYGKRIPLLHIKDGPLVPGQPHPHTAVGKGKMDFPAVIAAADPSVCQ
                     WLIVELDSCATDMMEAVADSYKYLVGSGLASGNK"
BASE COUNT          308 a          630 c          525 g          263 t
ORIGIN      
        1 ggcgtccagg tcgccgaagt agctgctcca gaccttgccg ccggccgcct tgattttgca
       61 ccgcggggct gtttgagcca tcagccggac atgttcggcg gtcgctccgc ccgccttgtg
      121 cataccggtg ctggtcttga gaaaatcaac ccctgcgtcc tgtccgagaa gctccagggc
      181 cgggcggcca aggtcgatga ccgcctccgc accgccgttc gcccactccc agcggaacgt
      241 cgttgcaggg tcgccgtcga tcagcgccgc tgccggccgc tggaccgccg cctgagcgga
      301 ttcagccgcg atccgggcgc cgtcaccacg cgccaggtcg accgcgcccg catcggccct
      361 caccagcgcc gccacacacc agagtactaa caacccaatc gcctccgccg aggaaaaggc
      421 tcctgacccg aatggtacgc acttgggact tgtggtgccc caggatcggc caacgaaact
      481 acctcgcacc ccaaaccagg gcgtcatcct gaggacccga ggcacggagg atctcggtcg
      541 gcgacggccc ccacacacag gcgcaggctc atgcctttgg cgctcggccg gttcgagccg
      601 agatccctcg ctccgcggat cctccgctca ggatgactcc cttgcttgtg gcctgaggtg
      661 ggcctcaggg ccggccgggg cgattggcgc gtaccatctc cccagacccc ttttgcgctg
      721 accccatctc cgcccgaagg tccgcacact ccaggcgcga atcctgctcc aggaaccgac
      781 taccgccgga gcccgccgcg gatccggccc actcacatgc ccgaactcca gataggggaa
      841 cgcacatgaa gccgatctca ctgcagctct actccgtgcg cgacgcctgc gctgtggact
      901 tcccgggaac ctgcaagaag gtggccgaca tcggctatac cggcgtcgag ctggccggtc
      961 tccacggcat gaccgccgcc caggccaagg ccatgttcga cgatctcggc ctcaaggtca
     1021 gcagccagca tgggcccctg ccgacccagg agaacgtgga acagctcgtc gccgactgca
     1081 agaccttcgg catcagccag accgtcaccg gcttcggccc cgatgacatg aagaccgtcg
     1141 acgccaccaa ggcctgcgcg gccaagatgc aggcgggcgc cgaactcctc aaaggcaccg
     1201 gcatcgagct ctgctaccat aaccattggt gggagttcga gccactcgag ggccagtgcg
     1261 ccttcgacat cctcatggcc gaggcgcccg atctgcgcgc ccagctcgat acgtactggt
     1321 gcacctttgg aggcgccgac gccgcggcgg tgctcaagcg ctacggcaag cgcatcccgc
     1381 tcctgcacat caaggatggg cccctcgtcc ccggtcagcc ccacccgcac accgccgtgg
     1441 gtaagggaaa gatggacttc ccggccgtga tcgccgccgc cgatccaagc gtctgtcagt
     1501 ggctcatcgt cgagctcgat tcgtgcgcga ccgatatgat ggaggccgtg gccgacagct
     1561 acaagtacct ggtaggctcg ggcttggcct cggggaacaa gtagctggcc gtctagggcg
     1621 ggccgggtag ggtctcacca gactgtggtc ccccctatcc ggcccctcag caaaccctcc
     1681 gaacaaccga ggaggggccg aatggaaggg gcccgcgaag cgggaa
//
LOCUS       DAOSPL010001166         1726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_69618_length_1726_cov_2.703770, whole genome shotgun sequence.
ACCESSION   DAOSPL010001166 DAOSPL010000000
VERSION     DAOSPL010001166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69618_length_1726_cov_2.703770"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PLQ54_17820"
     CDS             <1..615
                     /locus_tag="PLQ54_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95178.1"
                     /translation="PRVGDDVFGDDDVVRTGYAALTARGHTAKSIRERARREGKTEPE
                     VLCELMQDVVHDYSAFLAHVCRSAGIPRDRIYTHYTGISALPDAAIPADLREDGRNMP
                     LRAAVNDDSRPGITATAPWTDIGRAATIFRERGRRQWGAVEVEFTDATRPEEAALSYL
                     ESLSDSGARVICIFGWWEPVGHLFGVRRSGAVPAMKQWLAGGRK"
     gene            612..>1726
                     /locus_tag="PLQ54_17825"
     CDS             612..>1726
                     /locus_tag="PLQ54_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95179.1"
                     /translation="MSDDSPGPEQTAGWAHSPILTRNFVLGVLNGTLFMSTTRLVDPG
                     ALLSLLILRLTDSPRMIGVMAFASSLGWFWPAVFVANRAGNRPRKLSIYRDMAVVRII
                     LLWTAALVTFSHLAKDAPLVLFWLLAVAAFGQNSASGVAYVPFMEVVGKSVPTAHRGL
                     FLALRFSSAELLGVGVAALAGYFLSGASGLTYPANFGWMLSLAALLQSASMLMFILIR
                     EPAGAVESRQFPFSVFLRRGVRFFRRDRSLRNFTVLRLMNSFASMSLPFMAAFAKQDL
                     GVADSTIGYFMSVAAGAALVANLVWGRVGDTRGNRLVLIAYAGLSILPAVVALIAATT
                     SPGQVTLPLVGVADVRVVLVGVVFFLGGLTGPGRQFG"
BASE COUNT          232 a          555 c          614 g          325 t
ORIGIN      
        1 ccgcgggtcg gcgatgacgt gttcggcgac gacgatgtcg tgcgcaccgg ctatgcggcg
       61 ctcacggcgc gtgggcacac ggccaagtcg atccgggagc gggcgcgtcg cgaggggaag
      121 actgagcctg aggtcctctg cgagctgatg caggacgtgg tgcacgacta ctcggccttc
      181 ttggcccatg tctgtcggtc ggcgggcatt ccgcgggacc gcatctacac gcactacacg
      241 ggcatcagcg cgctgccgga tgcggccatc ccggccgatc tgcgggagga cggccggaac
      301 atgccgctcc gggcggccgt caacgacgac tcacggccag gcatcacggc cacggcgccg
      361 tggacagata tcggccgggc agcgaccatc ttccgcgagc ggggccgacg ccagtggggc
      421 gctgtcgagg tggagttcac cgatgccacg cggccggaag aggccgccct gagctatctg
      481 gagtcgctca gcgacagcgg ggcgcgcgtc atctgcatct ttggctggtg ggagcccgtg
      541 gggcacctct tcggcgtccg ccgcagcggc gccgtaccgg ccatgaagca gtggctggcg
      601 ggaggccgca agtgagcgat gacagccccg gcccggagca aactgccggc tgggctcact
      661 ctcccatcct gacgcgcaac ttcgtgctcg gcgtgctcaa tggcacactg ttcatgagca
      721 cgacgaggct cgtcgacccc ggagcgctgc tgagcctgct gatcctccgg ctgacggact
      781 ccccgcgcat gatcggcgtg atggccttcg ccagcagcct gggatggttc tggccggcgg
      841 tgttcgtggc caaccgggcg ggcaaccggc cgcgtaagct gagcatctac cgcgacatgg
      901 ctgtcgtgcg gatcatcctg ctgtggacgg cggctctcgt caccttctcg cacctggcga
      961 aggacgcccc cctcgtgctg ttctggttgc tggcggtcgc ggcgttcggt cagaactcgg
     1021 cgagcggcgt ggcctacgtg cccttcatgg aggtcgtggg caagtccgtg cccacggccc
     1081 accgcgggct gttcctggca ctccggttca gctccgccga gctacttggt gtcggcgtgg
     1141 cggcattggc gggctatttc ctctcggggg ccagtgggct tacgtaccca gcgaacttcg
     1201 gctggatgct ctcgctggcg gctctgctcc agtcggcgag catgttgatg ttcatcctga
     1261 tccgagaacc ggcgggcgcc gtggagagcc gtcaattccc cttctcggtg ttcctgaggc
     1321 gcggtgtccg gttcttccgc cgggaccgct cactccggaa cttcacggtc ttgcgtctga
     1381 tgaacagctt cgcgtcgatg tcgctgccgt tcatggcggc tttcgcgaag caggacctgg
     1441 gcgtcgcgga ctcgaccatc ggctacttca tgagcgtggc ggcaggtgcc gcgctggtgg
     1501 cgaacctggt ctggggaaga gtaggggata cgcgcggcaa tcgtctcgtg ttgatcgctt
     1561 acgcggggct ctcgatcctt ccggcagtcg tggcgttgat cgctgccacg acgtcgcccg
     1621 ggcaggtgac gcttcctctc gtcggcgtcg ccgacgttcg cgtcgtcttg gtgggcgtgg
     1681 tattcttcct gggagggcta acggggccgg gacgccagtt tggcga
//
LOCUS       DAOSPL010001167         1726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_69620_length_1726_cov_2.636146, whole genome shotgun sequence.
ACCESSION   DAOSPL010001167 DAOSPL010000000
VERSION     DAOSPL010001167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69620_length_1726_cov_2.636146"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="PLQ54_17830"
     CDS             <1..993
                     /locus_tag="PLQ54_17830"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOS95180.1"
                     /translation="FLFTGRPALEGLALARMRPVVIPARDGLDLVCYVSLPLDCPVDS
                     AGRPAAPLPLVLWVHGGPWYRDSWGYDPIHQWLANRGFAVMSINFRGSTGFGKAFLNA
                     SNREWAGRMHDDLLDAVEWAVREGIADPKRVAISGGSYGGYATLVGLTFTPEAFACGV
                     DIVGPSSLVTLMENVPDYWIPMMPVLRDRVGDHTTEEGRALLLERSPLTRADRICRPL
                     LIAQGANDPRVKQRESDQIVAAMREKSIPVTYLVYPDEGHGFVRPENRISFFAAMEAF
                     LAGQLGGRYEPVGGDLEGSSVQAVHGVEYVPGLAEAMAARRSTHDGGGSRCPNG"
     gene            978..>1726
                     /locus_tag="PLQ54_17835"
     CDS             978..>1726
                     /locus_tag="PLQ54_17835"
                     /inference="COORDINATES: protein motif:HMM:NF020120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HOS95181.1"
                     /translation="MPEWVAQWIWDDSGPAPRNYYLEARCSFELSAVPDHAELHCTAD
                     SRYVLFVNGARVGNGPVRGWATNWYYDSYDVASLLRPGENVLAMLVLQPGESNFQYVL
                     ARGGLLAQLEVDGATVATSERWRVRPSPGFSRRTPRVSCQQAFATEFDARREDLAWTA
                     PGYDDSSWATPTVVGPVGCEPWTNLRPRPIPFLTNEPVYPQRVISSTVVREWAHHVTL
                     HLAPVLYPGKVDSNPRTHRVLLATVARSPAPG"
BASE COUNT          251 a          572 c          601 g          302 t
ORIGIN      
        1 ttcctgttca ccggccggcc ggcgctggag gggctggcac tggcgcggat gcgtccggtc
       61 gtcatccccg cgcgcgatgg cctcgacctt gtctgctatg tctcactgcc cctcgactgt
      121 cccgtggaca gcgcgggacg gcctgcggcc ccgctgccgt tggtgctgtg ggtccacggc
      181 ggtccgtggt accgcgacag ctggggatat gaccccatcc accagtggct cgcgaaccgt
      241 gggttcgcgg tcatgagcat caacttccgc ggctccacgg ggttcggcaa ggccttcctg
      301 aacgcatcca accgcgagtg ggcggggcgc atgcacgacg acctgctcga cgccgttgag
      361 tgggcggtcc gtgaggggat cgctgacccc aagcgcgtcg ccatcagcgg cggcagctat
      421 ggcggctacg ccacgctcgt gggcctcacg ttcaccccgg aggccttcgc ctgcggagtc
      481 gacatcgttg gcccgtcgag cctggtcacg ctgatggaga acgtgccgga ctactggatc
      541 cccatgatgc cggtcctgcg cgaccgcgtg ggcgaccaca cgaccgaaga gggacgcgcc
      601 ttgcttctgg agcgatcgcc cctgacccgt gccgaccgga tctgtcgccc attgctgatc
      661 gcccagggcg ccaacgaccc gcgcgtgaag cagagggagt cggaccagat cgtcgcggcc
      721 atgagggaga agtcgatccc cgtgacatat ctggtctacc cagatgaggg gcacggtttc
      781 gtccggcccg agaaccggat ctcgttcttc gcggccatgg aggcgttcct ggccggtcag
      841 ctcggtggcc ggtatgagcc agtgggcggt gacctggagg gctcgtcggt ccaggcggtt
      901 catggggtgg agtatgtgcc cggtctggct gaggctatgg cggctcggcg gtcgactcac
      961 gatggagggg gttctcgatg cccgaatggg tagcccagtg gatctgggat gacagcgggc
     1021 cagcgccgcg caactattac ctggaggcgc gatgctcgtt cgagctctcg gctgtccctg
     1081 accatgccga gttgcactgc acggccgatt cgcgctatgt gttgttcgtc aatggcgcgc
     1141 gtgtcgggaa cggcccggtc cgtggctggg cgacgaactg gtactacgac tcgtacgatg
     1201 tagcgagcct cctgcggccg ggcgagaacg tgctggcgat gctcgtgctg cagcccggcg
     1261 agagcaactt ccagtacgtg ctggcccgag gaggcctgct ggcgcaactt gaggtggatg
     1321 gagctaccgt cgccaccagc gaacgctggc gcgtgcggcc gagcccgggg ttcagtcggc
     1381 gcacaccgcg tgtctcgtgt cagcaagcct ttgccacgga gttcgacgcc cggcgcgagg
     1441 acctggcctg gaccgcaccg ggctacgatg actccagctg ggccaccccg acggtggtgg
     1501 ggcctgtcgg ctgcgagccc tggacgaact tgcggccacg gcccatcccg tttctgacca
     1561 acgagcccgt ctatccgcag cgggtgatct cgtcgactgt ggtccgcgag tgggcccacc
     1621 acgtcactct ccacctggcg ccggtcctct accccggaaa ggtcgacagc aacccgcgaa
     1681 ctcaccgggt cctgctcgcc acggtggccc gcagcccagc gcctgg
//
LOCUS       DAOSPL010001168         1724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_69745_length_1724_cov_3.313960, whole genome shotgun sequence.
ACCESSION   DAOSPL010001168 DAOSPL010000000
VERSION     DAOSPL010001168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69745_length_1724_cov_3.313960"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="PLQ54_17840"
     CDS             complement(<1..1093)
                     /locus_tag="PLQ54_17840"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOS95182.1"
                     /translation="MSAELLARVQFGLTAGFHFLFPPLTIGLGWINVWILARYLRTRD
                     DLYRRMARFWIRVFALTFAVGVATGITLEFQFGTNWSEYSRFVGDIFGAPLAAEGILS
                     FFLESTFLAVLLFGWGRVSPRAYFVSSMLVAFGATLSAFWIIVANSWQQTPAGYTIDP
                     VSQRAVLTNFWAAVFNPSTVPRYLHTVDASLITGSLFVLGLSAWFILKGRHLDFAKES
                     MRIALIVALISSLAQLGLGHMHAVQVAETQPLKLAAGQALFETQRRAPILLFGIPDEE
                     NERIIGEISVPAILSILVAFDPNAEVKGLKDFQPKSDWPPVAVTYFSWHLMVICGTFF
                     IGLTVLGVYLARIGRLYTTRWYLWLAVLAI"
     gene            complement(1141..1644)
                     /locus_tag="PLQ54_17845"
     CDS             complement(1141..1644)
                     /locus_tag="PLQ54_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOS95183.1"
                     /translation="MSIRFNADEVFEIAEQIERNGARWYRMVADNAATADSKDMLLSL
                     AAMEDQHLARFSDMRRELTAAERKPVTFDPEGDAVLYLQAMADGSVFDMRADPTATLT
                     GKETLEEVLRTAISMENESILFYLGMKESVPADLGGARLEGIIKEEMGHVVTLSRRLA
                     AVRFPSR"
BASE COUNT          343 a          528 c          588 g          265 t
ORIGIN      
        1 ggatcgcgag aaccgccagc cacagatacc accgggtggt gtacaaccgg ccgattcgcg
       61 cgagatacac gccaagcacg gtgaggccga tgaagaatgt gccgcagatc accataagat
      121 gccatgagaa gtaggtgacg gccacgggtg gccaatcgct cttgggctgg aagtccttga
      181 ggcccttgac ctcagcgttc gggtcaaacg cgaccaggat gctcaggata gcgggcacgc
      241 tgatctcgcc gatgatgcgt tcgttctcct cgtcgggtat cccgaacagg aggatcgggg
      301 cgcgccgctg ggtctcgaac agggcctggc cagccgcgag cttgaggggt tgcgtctcgg
      361 ccacctgaac ggcgtgcatg tggcccaggc ctagctgggc cagcgatgag atcagggcga
      421 cgatcaaagc gatgcgcatc gactccttgg cgaagtcgag gtgccgccct ttcaggatga
      481 accacgccga gaggccgagc acgaacaggc tgccggtgat caggctcgcg tccaccgtgt
      541 ggaggtagcg gggcacggtg gacgggttga agacggcggc ccagaagttg gtcagtacgg
      601 cgcgctgact gacgggatcg atggtgtagc cagccggggt ctgctgccat gagttagcga
      661 cgatgatcca gaaggccgac agggtggccc caaaggccac cagcatcgag gagacaaagt
      721 aggccctggg tgacacgcgg ccccagccga agaggagcac ggccaggaat gtggactcga
      781 ggaagaagga gaggatccct tcggcggcca gtggggcgcc gaagatgtcg ccaacgaatc
      841 gcgagtattc ggaccagttg gtaccgaact ggaactcgag ggtgatcccc gtggcgaccc
      901 ccaccgcgaa ggtgagagcg aacacgcgga tccagaagcg ggccatgcgc cgatagagat
      961 cgtcgcgcgt tcgcagatac cgcgccagga tccagacatt gatccagccc agtccgatag
     1021 tcaggggagg gaacaggaaa tggaaccccg cggtgaggcc aaactgaacc cgcgccagca
     1081 gctcggctga cataggcctg tgtcacgggg gcgctacccg gcgcccccgt gacttccccc
     1141 ttaccgggag ggaaatcgga cggcggccag ccgccgggag agggtcacga catgacccat
     1201 ctcttccttg atgatcccct cgagccgggc cccgcccaga tcggctggaa ccgactcctt
     1261 catgcccagg tagaacaaga tggactcgtt ctccatggag atggccgtcc ggagcacctc
     1321 ctcgagggtc tccttccccg tgagcgttgc ggtggggtcg gctcgcatgt caaagacgga
     1381 cccatcggcc atggcctgca ggtagagcac cgcatcaccc tcgggatcga aggtgaccgg
     1441 cttccgctcg gcggccgtga gttcccggcg catgtcggag aaacgtgcca ggtgctggtc
     1501 ctccatggcg gccagagaga gcagcatatc cttgctgtcg gccgtggccg cgttgtcggc
     1561 caccatgcgg taccagcggg caccgttgcg ctcgatctgc tcggcgatct cgaagacctc
     1621 atcggcgttg aaccggatgc tcatgccccg gcccctccct cagtcgcggg cgcgggcatg
     1681 acgaagaccc gcgtggcgag ctcgcggtcg atggcgaaca gccc
//
LOCUS       DAOSPL010001169         1722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_69862_length_1722_cov_2.511698, whole genome shotgun sequence.
ACCESSION   DAOSPL010001169 DAOSPL010000000
VERSION     DAOSPL010001169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69862_length_1722_cov_2.511698"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="PLQ54_17850"
     CDS             <1..1373
                     /locus_tag="PLQ54_17850"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOS95184.1"
                     /translation="ADAALVAAGHADPEATVTMRAVFAVRFLVLSLRHMRRVRPAILV
                     ALADSPPRRRILGHADSYAAASSAVVVVSLLLGYGSLAHVAAKGLLWTGVALYVGYYL
                     WRLIGRHTAERVPADADAGTADPDEAEIRELKDVGWGLIRAITRTGVALAVCYAAAQA
                     WGVHRPHLEYAYTALSRGAVSVQGTEVSLLSLLRAVVLVWIFIYIGRVTQRLIGASRG
                     LRLRVGEGPRYALAHLWFYAACTVGVLWAMLVAGFQWSVLTVFAGMAGIGLGFGLQDV
                     VRNFVSGLILLVERPIVMGDLIEAGGFKGFVVQIGLRSTTLRAFDNTRHVLPNSSLIS
                     EKVTNFAAADQKVRCEIDIGVAYSTDVVMAQQVLLRAADASPEILDDPAPVAMVTGFG
                     DSAVNLRLLAWLSGRDSSAAVSVRSLLAARILEEFRKEGIEIPFPQRDIHVRTRGGID
                     DVPGAG"
     gene            1405..>1722
                     /locus_tag="PLQ54_17855"
     CDS             1405..>1722
                     /locus_tag="PLQ54_17855"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HOS95185.1"
                     /translation="MRFRPQRYWVTPARREEVRQAVSTHSSRHAGYIVLTIVSTVIAA
                     YGLMANSAAVVIGAMLVAPLMGPIIGVAFGVATGDTRILRRAAATELVGVIIAVGLAT
                     LIGL"
BASE COUNT          241 a          570 c          598 g          313 t
ORIGIN      
        1 tagccgatgc ggccctcgtc gccgctggcc acgcagaccc ggaagccacg gtgaccatgc
       61 gggccgtctt cgccgtgagg ttcctcgtcc tgtcactgcg gcacatgcgg cgcgtccggc
      121 cggctatcct cgtggcgctc gccgatagcc cccctcggcg ccggatcctg ggccacgccg
      181 acagctatgc cgccgcttcc tcggccgtgg tggtggtctc gcttctgctc ggctacggca
      241 gcttggcgca tgtggcggcc aaggggctgc tctggactgg cgtcgcgctc tatgtgggct
      301 actacctgtg gcgcctgatc ggccgccata cggccgaaag agtgccagcc gacgcggacg
      361 ccggcacggc ggaccccgac gaggctgaga tccgcgagct caaggacgtc ggctggggtc
      421 tcatccgcgc catcacgcga accggcgtgg ccttggccgt ctgctacgcg gccgcgcagg
      481 cgtggggcgt ccatcgtccg cacttggaat acgcatacac ggccctctct cgaggcgccg
      541 tgagtgtcca ggggaccgag gtgtcgctcc tcagcctgct gcgcgccgtg gtgctggtct
      601 ggatcttcat ctacatcggc cgggtaacac agcgcctcat cggcgcgtcc cgcgggctgc
      661 ggttgcgtgt gggcgaaggc cctcgctatg ccttggccca tctgtggttc tacgcagcat
      721 gcaccgtcgg tgtgctctgg gccatgctcg ttgccggttt ccagtggagc gtgctgaccg
      781 tgttcgccgg gatggcgggc atcgggctgg gcttcggcct acaggacgtg gtccgcaact
      841 tcgtgtccgg gctgatcctc ctcgtcgaac gccccatcgt catgggcgac ctgatcgagg
      901 cgggtggctt caaaggcttc gtggtacaga tcggtctccg cagcaccacg ctccgcgcgt
      961 ttgacaacac acgtcatgta ctgcccaact cttcactcat ctcagagaag gtgaccaact
     1021 tcgctgcagc agaccagaag gtgcgatgcg agatcgacat cggggtcgcc tactcgaccg
     1081 acgtagtgat ggcccagcag gtgctgctga gggcggcgga cgcgagcccg gagattctgg
     1141 acgaccccgc tccggtggcc atggtgactg gcttcgggga cagcgcggtg aacctacgac
     1201 ttctcgcatg gctgagcggg cgggactcca gcgcggccgt gtccgtccgc agcctcctcg
     1261 cagcgcggat cctcgaggag ttccgcaagg aaggcatcga gattccattc ccgcagcgcg
     1321 atatccacgt gcggacgcgc ggcgggatcg atgacgtgcc aggcgccgga tgagggtggc
     1381 gccggcgcgt gagggaggca tgctgtgcgg tttcgtcccc aacggtactg ggtcacgccg
     1441 gcgcggcgag aggaagtgcg acaggcggtc tcgacgcatt cctcgcgcca cgcggggtac
     1501 attgttctca cgatcgtctc gaccgtgatc gcggcctacg gactgatggc aaacagcgcg
     1561 gcggtcgtga tcggggccat gctggtggct ccgctgatgg gcccgatcat cggcgtcgcc
     1621 ttcggcgtgg ccacgggcga cacgcgcatc ctacgccgcg cggcggccac tgagctcgtc
     1681 ggcgtgatca tcgccgtggg cttggcgacc ctgatcgggc tg
//
LOCUS       DAOSPL010001170         1721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_69890_length_1721_cov_3.064826, whole genome shotgun sequence.
ACCESSION   DAOSPL010001170 DAOSPL010000000
VERSION     DAOSPL010001170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69890_length_1721_cov_3.064826"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            754..>1721
                     /locus_tag="PLQ54_17860"
     CDS             754..>1721
                     /locus_tag="PLQ54_17860"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS95186.1"
                     /translation="MTTQGGLAKAAEYWGDHQRLDFYARGGLVWWDVPAVRRRLDQLI
                     SGDPECDWIAHLKRAHLAGRAPVARALSLCCGDGRVERSLVLDGVARECEGVDASPGA
                     LERARGLADEAGVCGLSYRLCDVNQLEADEGSYDLVVAHQALHHIEALEHVAHTCRKA
                     LRPGGLMVAHEYVGPNRLATTPRDLELMNAALRLLPERLRTSVSWQRSGRVGPGARRT
                     PLQWARKAWLKLVHGELGAALVHRRELARLRSTGQAYIKSEIRPVIGDELAADDPTEA
                     IRSADILGVLEAEFEFVQFAPLRGALLHRVLDDIAGDFDADDAEAT"
BASE COUNT          284 a          592 c          575 g          270 t
ORIGIN      
        1 gggctggtcc atgatgatct cgtgctggcc gacacgcgca gacatgcgcg gtcccccctg
       61 gagcgtcact tcgagttcgc gtggcatggc ggtctccttt gctgaatcgg gcctgtggct
      121 gacccactat tgtgtacagc gtcttcttac ccggaggata tccgatctgg ggaccgatga
      181 ggccgggtcc cgaagaaggc aaggagatcg gtgccgatcg ccgggcccag tctgcggcaa
      241 gacgcggccg atccggccga tcccgcctgt ctgcccgcca aagatcatgc tcgtggtgct
      301 ccggagcccg aggcttccgc acgcggaaag caacccggca cgaaaacagg tgggtatttc
      361 gctcactggc cgaaaccact ccacacgcaa tgtgtcatag aagccgtggg cagcgcgaac
      421 tatagatcag cgtagaccca cgccgccgca catctccttt cgcaccggcc gtcgcctgac
      481 cagcgacggc cgaactcttt tccaggccgg atcggccgtt tgtcatatcc gacacggtcc
      541 ctgcgaacca gataacagcg tagacccaat gcgactccgc atctcctttc gcaccggccg
      601 ccgactgacc accggcggcc tctctatttg ggccccagcg ccctgacgcc ggctgtctgc
      661 cacgtccggc gacggtaccc cggaagtgat ccgctccctc ccgccgaatg acgcgtcgat
      721 ggtacacgcg cagaagggca ggacgagagc gagatgacga cgcagggcgg ccttgccaag
      781 gcggcagagt actggggaga ccatcagcgg ctcgacttct acgcccgcgg cggactggtc
      841 tggtgggatg tgcctgcggt gcgccggcgc ttggatcagc tcatcagtgg ggacccggag
      901 tgtgactgga tcgctcacct gaagcgcgcc cacctggcag gccgtgcgcc ggtggcgcgg
      961 gcgctcagcc tctgctgtgg cgacggccgg gtcgagcgct ccctcgtctt ggatggagtg
     1021 gcacgggaat gcgagggcgt cgacgcctca cccggcgccc tcgaacgcgc ccgcgggcta
     1081 gcggacgagg ccggcgtctg cggcctttcc taccgcctgt gcgatgtgaa ccagctcgag
     1141 gccgacgagg gatcctatga cctcgtggtc gcccaccagg ctctgcacca catcgaggcg
     1201 ctcgagcatg tggctcacac ctgccggaag gcgctccggc cgggcgggct catggtcgcg
     1261 cacgagtacg tgggaccgaa ccggctcgca accacgccac gcgacctcga gctcatgaac
     1321 gccgcgctgc ggctgctccc ggagcgcctt cgcacgagcg tgagctggca gaggagcggt
     1381 cgagtcggcc cgggcgcgcg gcgcaccccg ctgcagtggg ccaggaaggc atggctgaag
     1441 ctggtccacg gcgaactcgg ggcagccctg gtccaccgcc gagagctcgc gcggctccgt
     1501 tcgaccggcc aggcgtacat caagagcgag atccggccgg taatcggcga tgagttggcg
     1561 gccgacgacc ccaccgaggc gatccggtcg gcggacatcc tcggcgtcct ggaggccgag
     1621 ttcgagttcg tccagttcgc gcccctgcgc ggagcccttc tgcaccgcgt gctggacgac
     1681 atcgccggcg atttcgatgc agacgacgcc gaggccaccc a
//
LOCUS       DAOSPL010001171         1719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_70005_length_1719_cov_2.516827, whole genome shotgun sequence.
ACCESSION   DAOSPL010001171 DAOSPL010000000
VERSION     DAOSPL010001171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70005_length_1719_cov_2.516827"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PLQ54_17865"
     CDS             complement(<1..406)
                     /locus_tag="PLQ54_17865"
                     /inference="COORDINATES: protein motif:HMM:NF037707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HOS95187.1"
                     /translation="MASAMNGGLEWLWAKSPAPGGPPDRYHPLVCHMLDVAAVAEALW
                     DRWLKPPVRRRLGRLLGADDGEARTWLALVAGLHDLGKCTPAFQVLWPGALPNLAAAG
                     LTGSPIERIGHGTASCRLIADELRACCPGLPAR"
     gene            735..>1719
                     /locus_tag="PLQ54_17870"
     CDS             735..>1719
                     /locus_tag="PLQ54_17870"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS95188.1"
                     /translation="MTRPHILLLGASVGAGHDSICSAIAQFLTQLRPDARVTVLDFVR
                     EFVGVEAAERGRRQYLRAVCCAPRLYGLYHWVAGRIPPRSALQRFINRGGQQAFREYY
                     ERERPDVILCNYPAAVGACGELRRRGEIGCPVVTMVGDYDISNQWVHPYNDLTLATAE
                     FVAEGMIARGLDPARVITTGLCIRPPFAEDHDRAALRRQWGVPGDAPVVLVLPGAFGM
                     MGGLGEVWGALERLRGLHALIVTGHSRSVREWFTARVAGQEDRLRVLGFVTEMHELMA
                     LADFCITKAGGVTCTEAMALRLPMVIHRPIPGQEGANARYVVETGVGLVTRN"
BASE COUNT          256 a          538 c          631 g          294 t
ORIGIN      
        1 atcgcgccgg aagaccggga cagcatgcgc gcagctcgtc ggcgatgagc cggcagctcg
       61 ccgtcccgtg gccgatgcgc tcaatcgggc ttcccgtcag gcccgccgcg gccaagttcg
      121 ggagagcgcc cggccatagg acctggaacg ccggcgtgca cttgcccagg tcgtgcaggc
      181 ccgcgaccag ggcgagccac gtgcgcgcct ccccatcgtc agcgccgaga aggcggccca
      241 gccgtcgacg gaccggcggc ttcagccagc ggtcccagag tgcttctgcg accgcggcga
      301 cgtcgagcat gtggcacaca agcgggtggt agcgatcggg cgggccgccg ggcgcagggc
      361 tcttggccca gagccactcg agtccgccgt tcatcgcact ggccatggca tgccatttcc
      421 tcgcttggag tacgagcggt gtgtttcaca ctattccctt gcgttggttc cccaccttgc
      481 gtccatgacc tgcagtaccg tagcacagtc cgctcaggca tcacgctggc cagggaaggg
      541 gttggccttc ccattgaccg gccatataag catatgagca gaccactgac agctgcggct
      601 ggggcttgcg gcgagggcgg cggtgctgtt gtgtgcccgc cggacttggt cggaacgcgg
      661 gagcagctta tgataccgcg ttaggccgcg cgcggattgg gcgcggagtc tggtagcggg
      721 agacattcgg cgagttgacg cgccctcaca tcctcctcct cggcgcctcg gtcggtgcgg
      781 ggcatgactc gatctgcagc gccattgctc agttcttgac ccaactgcgc cccgacgcca
      841 gggtcacggt gctggacttc gtccgcgagt tcgtcggcgt cgaggcggcg gaacggggcc
      901 ggcgccagta tcttcgcgcc gtgtgctgtg cgccgcggct ctacggtctg taccactggg
      961 tggcgggccg gattccgccc agaagcgcgc tccagcggtt catcaaccgg ggcggacagc
     1021 aagccttccg cgagtactac gagcgcgagc ggcccgacgt gatcctgtgc aactacccgg
     1081 cggcggttgg cgcctgcggc gagctgcggc ggcgaggcga gatcggatgc cccgtggtca
     1141 cgatggtggg cgattacgac attagcaatc aatgggtcca tccgtacaac gacctcaccc
     1201 tagcgacggc ggagttcgtg gccgagggga tgattgcgcg gggcctcgat ccggcacgcg
     1261 tcatcaccac ggggctgtgc attcggccac cgttcgctga ggaccacgac cgggcggctc
     1321 tgcggcgcca gtggggcgtg cccggcgatg cgccggttgt gctcgtgctg ccgggagcct
     1381 tcgggatgat ggggggcctc ggcgaggtgt ggggcgcgct ggagcggctt cgggggctgc
     1441 acgcgctgat tgtgaccgga cacagccgga gcgtgcgcga gtggttcacg gcccgggtgg
     1501 ccggccagga agaccgcctg cgggtgctgg gtttcgttac ggagatgcat gagctgatgg
     1561 cgctggctga tttctgtatc accaaagcag gcggggtcac gtgcaccgag gccatggcgc
     1621 tgaggctgcc gatggtgatt caccggccga tcccgggtca ggagggggcc aatgcgcgtt
     1681 acgtggtgga aaccggggtg ggattagtaa cgcgtaatg
//
LOCUS       DAOSPL010001172         1718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_70066_length_1718_cov_2.303668, whole genome shotgun sequence.
ACCESSION   DAOSPL010001172 DAOSPL010000000
VERSION     DAOSPL010001172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70066_length_1718_cov_2.303668"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PLQ54_17875"
     CDS             <1..462
                     /locus_tag="PLQ54_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95189.1"
                     /translation="MSFTPAYLEIDATYLVPSASPLRTADEVDREGVRVAAPARENFE
                     LFLSRTAQPTPYAHFILNPANVQWDAESAEGGPDRAGWNAEWQSGTRIGDTAWTAELV
                     IPWTALGGKPEPGETRRANVCRARIPDRELSAWSTVVDGFVEPMCFGEWAF"
     gene            476..>1718
                     /locus_tag="PLQ54_17880"
     CDS             476..>1718
                     /locus_tag="PLQ54_17880"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOS95190.1"
                     /translation="MPIDYRPFDANCVVGRQLYQGPGAPCTASDLLAEMDHYGIAEAL
                     VVDSLSRLNHPFDGNERVLSTCAQSPRLHPAWSALPAAGTGEMPPPDEMLRRMREERV
                     GALFLYPRQYRFSLADWCVDPLLEPLAEAGTPVFINPNDIGAGVHPEWDETEWDKVVA
                     LCRRWPRLPVIVSEFRIRRAMRTVYRAFDACPNLRIELSGYWLYRGIEYITRTWGPEL
                     LFGSNWPTFGPHMTLAALSCAEIDDGAKRRIAGDNLRELIEWCGARHEPAWVPPAEDA
                     FAQFGRTGKRPESMTFMDCHGHYGGRFSHYHVPDGTLEGTIAEMDRLGVERVLLFSFA
                     GVGSDEVHGNDRVAEALAAYPDRFVGLTLLNPHRGPEEMTAELERCAALGFRGIKLIP
                     YYQGYPEEGPHIDVACRWADEH"
BASE COUNT          275 a          575 c          585 g          283 t
ORIGIN      
        1 atgagtttca ccccggccta tctcgagatc gacgcgacct acctcgtgcc cagcgcctcg
       61 cccttgcgca ccgcggacga agtggaccgc gagggggttc gcgtggccgc gcccgcgcgc
      121 gagaacttcg agctgttcct gagccgcacc gcccagccca ccccctacgc ccatttcatc
      181 ctcaacccgg cgaacgtcca gtgggatgcc gagtccgccg agggcggacc ggaccgcgcc
      241 ggctggaacg ccgagtggca gagcgggacg cgcatagggg acaccgcatg gacggctgag
      301 ttggtgatcc cctggacggc tctgggcggc aagccggagc cgggtgagac gcgccgcgcg
      361 aacgtctgcc gcgcgcgcat acctgaccgc gagctgagcg cgtggtcaac ggtggtcgat
      421 ggctttgtcg agcccatgtg cttcggcgag tgggccttct gagagggtgg gtcgagtgcc
      481 gattgactac cggccgttcg acgcgaactg cgtcgtcggt cgccagctgt atcaggggcc
      541 cggggctcct tgcacggcca gtgacctcct ggccgagatg gaccactatg gcatcgccga
      601 ggcactggtg gtcgacagcc tcagccgcct gaaccatccg ttcgatggca acgaacgcgt
      661 gctgtccaca tgcgctcaga gcccacgcct gcatccggca tggtcggcgc tgcccgccgc
      721 cggtaccggc gagatgcccc cgcccgacga gatgctgcgg cgcatgaggg aggagcgggt
      781 gggagcgctc tttctctacc cgcgccagta ccggttcagt ctggccgact ggtgcgtgga
      841 tccactgctc gagccgctgg ccgaagccgg cacgccggtg ttcatcaacc caaacgacat
      901 cggggctggt gtgcacccgg agtgggacga gaccgaatgg gacaaggtgg tggctctgtg
      961 ccgccggtgg ccccgcctgc cggtcatcgt cagcgagttc cgcatccgtc gcgccatgcg
     1021 gacggtctac cgcgccttcg acgcctgccc caacctgagg atcgaactca gcggctactg
     1081 gctctaccgg ggcatcgagt acatcacgcg tacgtggggg cccgagctgt tgtttggatc
     1141 gaactggcca acgtttgggc cacacatgac cctggcagcc ctatcctgtg ccgagatcga
     1201 tgacggggcg aagcgcagga tcgctggcga caacctgcgg gagctcattg agtggtgcgg
     1261 cgcacggcac gagcccgcct gggtcccgcc ggccgaggac gcgttcgcac agttcggccg
     1321 caccgggaaa cgccctgaga gcatgacctt catggactgc cacgggcact atggtgggcg
     1381 tttcagccac taccatgtgc ccgacggtac gctcgaaggc accattgccg agatggatcg
     1441 cctgggagtc gagcgcgtgc tcctgttcag tttcgccggt gtcgggtcgg atgaggtgca
     1501 cggcaacgac cgcgtggccg aggctttggc ggcctatcct gatcgcttcg tcgggctcac
     1561 gctcctgaac ccgcaccgag ggccggagga gatgaccgcc gagctcgaac gctgtgccgc
     1621 cctgggcttc cgcggcatca agctaatccc ctactaccag ggctaccccg aggaaggccc
     1681 tcacatcgat gtggcgtgcc ggtgggctga tgagcacc
//
LOCUS       DAOSPL010001173         1717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_70109_length_1717_cov_2.648014, whole genome shotgun sequence.
ACCESSION   DAOSPL010001173 DAOSPL010000000
VERSION     DAOSPL010001173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70109_length_1717_cov_2.648014"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PLQ54_17885"
     CDS             complement(<1..751)
                     /locus_tag="PLQ54_17885"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HOS95191.1"
                     /translation="MQIPEHIFRGYDMRGRVGPEVTPELVELVGKAYATYLWDQSGAK
                     PDVIVGRDLRPSSEELSEALIAGILSTGADAVDVGAAPTPVVNFSYHYLRKTGVAMVT
                     ASHNPSHDNGLKLKRAPQRPICGADLQDVLAIAKSGRFRTGKGLRAEASTIPAYIDAI
                     VERLRPCRGLRVVVDGGNGSGSQTTPDLLRALGCEVIEIFCDPDGTFPNHHPDPSVPA
                     NMVDLQAKVRETGAAIGFGLDGDGDRLGVVDD"
     gene            complement(845..>1717)
                     /locus_tag="PLQ54_17890"
     CDS             complement(845..>1717)
                     /locus_tag="PLQ54_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95192.1"
                     /translation="RYGFDRPINGYGGLRFLGLHDGYQRGEAAARVTQESGLDYETAF
                     MRAWDAVDRHARQAGWPTIYYAMCDETRVRDVAERELDFMKLMAQVTAQFPKTVRSSG
                     SYSVDFRDRPTDGEDLKTWHQRFFEALDISSLNVHDETVIAEARALGKEVHIYNQGQT
                     RYSFGLYQWNEFTKGVRARWQWHLNVLHGYQFFDLDGREPDTAMICYGSRGIYPTIHF
                     ERCREGAEDFYLYQTLAHAIERRSAAGQTEREATELLTRLTDGMQLNQTTPPDGYNPD
                     ATKLEVIEALERLR"
BASE COUNT          268 a          573 c          565 g          311 t
ORIGIN      
        1 catcgtcgac gacgcccagg cggtcgccat cgccatcgag gccgaacccg atggccgcac
       61 cggtctccct gaccttcgcc tgaaggtcca ccatattcgc cggcaccgac gggtcggggt
      121 gatggttcgg aaaggtgccg tcggggtcgc agaagatctc gatgacctcg cagcccaggg
      181 ctcggaggag gtcgggggtg gtctgcgacc cgctaccatt ccccccatcc acgaccaccc
      241 ggagccccct gcacggcctg agacgttcga cgatggcatc gatgtaggcc gggatggtgc
      301 ttgcctcggc tcggaggcct ttgcccgtcc tgaaacggcc actcttggcg atggcgagaa
      361 cgtcctgcag gtcggccccg cagatggggc gttggggagc gcgcttgagc ttcaacccgt
      421 tgtcatgcga tgggttgtgg ctagcggtca ccatggcaac gcccgtcttg cggaggtagt
      481 ggtatgagaa gttgaccacg ggtgtgggcg cggcgccgac gtcgacggca tcggcacccg
      541 tcgatagaat gccggcgatg agcgcctccg acagctcctc ggaactgggc cgcaggtcgc
      601 ggccgactat gacatcgggc ttggcccccg actggtccca gaggtaggtc gcgtaggcct
      661 tgcccacgag ctcaacgagc tccggcgtga cttccggccc cacgcggccg cgcatatcat
      721 agcctcggaa gatgtgctca gggatctgca ccggtagcct cctcacgtaa tcgcccctgg
      781 tgactcaacc cacaggcagg gccgcagcgc gccacgcacg tctgtcaacc gtgggcaccg
      841 ccccctacct cagccgctcg agggcctcga tcacctcgag cttggtggcg tctgggttat
      901 agccgtccgg tggtgtggtc tggttcagct gcatgccgtc cgtcagccgc gtcaacagct
      961 ccgtggcctc gcgctcggtt tggccggcgg cggaccgccg ctcgatcgcg tgtgccagcg
     1021 tctgatacag gtagaagtcc tccgcgccct cgcggcatcg ctcgaagtgg atggtcgggt
     1081 agatgccccg ggagccatag cagatcatcg ccgtgtcggg ctcgcggcca tcgaggtcga
     1141 agaactgata gccgtgcagc acgttgaggt gccactgcca ccgggctcgc acgcccttgg
     1201 tgaactcgtt ccactggtac agcccaaagc tgtagcgcgt ctggccctgg ttgtagatgt
     1261 ggacctcctt gcccagcgcc cgtgcctcgg cgatcacggt ctcgtcgtgt acgttgaggc
     1321 tgctgatgtc gagcgcctcg aagaaccgct ggtgccaggt cttcaggtcc tccccgtctg
     1381 tgggccggtc gcgaaagtcc acgctgtagg agccgctgga gcgaacggtc ttggggaact
     1441 gcgccgttac ctgcgccatg agcttcatga agtcgagctc gcgctcggca acgtcgcgga
     1501 cgcgcgtctc atcacacatg gcgtagtaga tggtgggcca acccgcctgg cgtgcgtgcc
     1561 gatcgacggc gtcccaggcc cgcatgaagg cggtctcata gtcgagcccg ctctcctgag
     1621 tcaccctcgc cgccgcctca cccctctgat agccgtcgtg caggccgagg aatcgcaggc
     1681 cgccatagcc gttgatgggc cggtcgaacc catagcg
//
LOCUS       DAOSPL010001174         1712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_70370_length_1712_cov_3.176222, whole genome shotgun sequence.
ACCESSION   DAOSPL010001174 DAOSPL010000000
VERSION     DAOSPL010001174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70370_length_1712_cov_3.176222"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PLQ54_17895"
     CDS             <1..672
                     /locus_tag="PLQ54_17895"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOS95193.1"
                     /translation="QSLTERLPGVIRSYKIVTPEESLSRVCQSLGIQPDEGLRKRNPL
                     PASIEIELVRPQEYAMVVTAASDSQRVDEVKAGQKVLENLMALKRLGIAVGAALVFLL
                     GAAALLTISNTIRLTIYARRREIDIMQLVGATQSFIRAPFTLEGMFHGVAGAAVGGAI
                     LIASYVNLRLWVTSDQSLLPFLEGLLSVGTVSDLALCWGVLMVAGAAFGAVGSWLSVT
                     RYLHA"
     gene            787..>1712
                     /locus_tag="PLQ54_17900"
     CDS             787..>1712
                     /locus_tag="PLQ54_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95194.1"
                     /translation="MGNAKRSRLVRQMALTLAAVAMVPVVYAAGASGLRARRDSIFSQ
                     MRAVRAQRADAASEAERCRQELRASEERLAAADRSYKRAVAEKRKAQEGIQQAAEARD
                     RAMAELAEAQSLLESRLVAMFKAGSGSYTEVVLGAQDLTDMTTRAYLCERIVEGDLEL
                     IRHMTARKQAAELEEQRLVVEESRLRKAEAALAEAKRKRAAEAEHQRALLEEQKQIVA
                     EIDQELAELAAASAQIEQELRALAAQRGNLQPSQAWTGSWRAPVSGRITSGYGMRIHP
                     ISRRLANHTGVDISAPTGTDVAAAGSGVVCKAA"
BASE COUNT          306 a          543 c          594 g          269 t
ORIGIN      
        1 cagtctctga ccgaacgcct gcccggggtc atccggtcct acaagatcgt gacgcccgag
       61 gagtcactga gccgcgtctg ccagagcctc ggtatccagc ccgacgaggg actgcggaag
      121 cggaaccctc tgccggcctc gattgagatc gagctcgtgc ggccccagga gtacgccatg
      181 gtggtcacgg cggcgagtga ctcccagagg gtcgacgagg tgaaggcggg acagaaagtc
      241 cttgaaaacc tcatggctct caagcggctg ggcatagccg tcggtgccgc cctggtcttc
      301 cttctcggcg ccgccgcgct gctgaccata tcgaacacca ttcgtctgac gatctacgcg
      361 cgtcggcgtg agatcgacat tatgcagctc gttggggcga cccagagttt catccgcgcc
      421 cctttcacgc tcgagggaat gttccacggt gtggcgggag cggcggtggg tggggcgatc
      481 ctcatcgcca gctacgtgaa cctgcgcctg tgggtcacct cagaccagtc gctgcttcca
      541 ttcctggagg gcctactcag tgtgggcacg gttagcgact tggctttgtg ctggggtgtc
      601 ctaatggtag ccggcgccgc atttggtgcc gtggggagct ggttatcggt aacacggtac
      661 ctccacgcct aggccggccg gctaccgccg gcggggcagg agtgacgccg caacgccacg
      721 aacacatccg aagatggcga tctgtgatcg tacgggtagt cgtcccgcat ctctggagag
      781 ggtgcgatgg gcaacgcgaa gcgaagcaga ctggtgaggc agatggcgct gacgctggcg
      841 gccgttgcca tggtgccggt cgtctacgcg gccggcgcat ccggtctccg tgctcggcgc
      901 gacagcatct tctcgcagat gcgcgccgtt cgcgcccagc gtgcggacgc cgcctccgaa
      961 gccgagcggt gccggcaaga gctccgtgca tccgaggagc gactcgccgc ggccgatcgc
     1021 agctacaagc gcgcggtggc cgaaaagcgc aaggcccagg aggggatcca acaagccgcg
     1081 gaagcccggg accgagccat ggccgagctg gccgaggcgc agagcctgct cgagagccgg
     1141 ctcgtcgcca tgttcaaggc cggcagcgga tcctataccg aggtggtcct cggtgcccag
     1201 gacctcaccg acatgacgac tcgcgcctac ctatgtgagc gcatcgttga gggtgacctc
     1261 gaactgatcc gccacatgac cgcgcggaaa caggcggcag aactcgagga gcagaggctg
     1321 gtcgtcgaag agtcgcggct ccggaaggcc gaggcggctc tggccgaagc caagcggaaa
     1381 cgagccgccg aggccgagca ccagcgggcg ctgctcgagg aacagaagca gattgttgcc
     1441 gagatcgacc aggaactcgc agagctggcg gcggcgtctg cccagatcga gcaagaactg
     1501 agggccctcg ccgcgcagcg aggcaacctg cagccgagtc aggcatggac gggctcgtgg
     1561 cgcgcgcccg tgtcgggcag gattacctcg ggctatggca tgcgcattca cccgatatct
     1621 cgccgactgg ccaaccacac cggtgtcgat atcagcgctc cgaccggcac ggacgtggcc
     1681 gcggcgggca gcggtgtcgt ctgcaaggct gc
//
LOCUS       DAOSPL010001175         1712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_70375_length_1712_cov_3.089922, whole genome shotgun sequence.
ACCESSION   DAOSPL010001175 DAOSPL010000000
VERSION     DAOSPL010001175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70375_length_1712_cov_3.089922"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="PLQ54_17905"
     CDS             <1..898
                     /locus_tag="PLQ54_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOS95195.1"
                     /translation="ITFGFALAEYVSRLGERIEAIQGAAANLRGKISGAVGAYNASSL
                     LIDDPAAFEEEVLAELGLRPAWHSTQIVAPEHVTDYMHTLTSCFSVLAALADDMRHLQ
                     RTEISEVGEAFEASQVGSSTMPHKRNPWNFENVKSLYKAFMPRMVSVYLDQLSEHQRD
                     LTNSATNRFLPETIVGLMNASERLRRIMSRLVVDGEQMRANLELTAGMCLAEPLYILL
                     AAQGHPDAHEAVRRLTLEAERTGRRLWDVAAESDIGPYLDRMTPAQRAVLQDPALYQG
                     IAALRVDAICDHYAERLGLAGR"
     gene            923..>1712
                     /locus_tag="PLQ54_17910"
     CDS             923..>1712
                     /locus_tag="PLQ54_17910"
                     /inference="COORDINATES: protein motif:HMM:NF017215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HOS95196.1"
                     /translation="MGDTLRVGIDVGGTFTHAVAIDAMTQSLRASVRVPTTHRATRGV
                     AEGVCEALRALLARLGQSPESVALIAHSTTQATNALLEGDVAPVGVLAVSVGRSGMSR
                     VTSARAERETDVGDTPIGEGGHRSVPVRHVHVECTSEELSDAVGAAIGTLKAEGCEVI
                     VAAEAFSIEDPSNERRVAELAIQAGLPATATSDISLLHGLRQRTRTAVVNASILPKMS
                     EAARCTEDAARALGFQAPLLIVRSDGGTMSAEEATRRPLLVNATP"
BASE COUNT          272 a          566 c          595 g          279 t
ORIGIN      
        1 gatcaccttc gggttcgcgc tggccgagta cgtgagccgc ctgggcgagc ggatcgaggc
       61 gatccagggg gctgcggcga acctccgggg caagatctcc ggtgcggtgg gcgcctacaa
      121 cgcgtcgagc cttctgattg acgatcccgc ggcgttcgag gaggaggtcc tggcggagtt
      181 ggggctgagg cccgcctggc actcgaccca gatcgtcgcg ccagaacacg tcaccgacta
      241 catgcatacg ctgacctcgt gcttctccgt cctggctgcc ctggcggatg acatgcgcca
      301 cctgcagcgg accgagatct ctgaggtggg tgaagctttc gaggccagcc aggtcggatc
      361 gtcaaccatg cctcacaagc ggaacccgtg gaacttcgag aacgtcaagt cgctctacaa
      421 ggcgttcatg cctcgcatgg tctcggtgta cctcgaccaa ctctcggagc accagcgtga
      481 cctgaccaac tcggcaacga atcgcttcct gcccgagacg atcgtgggtc tgatgaatgc
      541 gtccgagcgg ttgcggcgca tcatgtctcg gctcgtggtc gacggcgagc agatgcgggc
      601 caacctggag ctcacggcag gcatgtgctt ggcggagccc ctgtacatcc tgctggccgc
      661 ccagggacat cccgatgccc atgaggccgt ccggcggctc acgctggagg ccgagcgcac
      721 gggccgccgg ctctgggacg tggcggcaga gagcgacatc gggccctacc tcgatcggat
      781 gacaccggct cagagggcgg ttcttcagga cccggcgctc taccagggca tcgcggcgct
      841 tcgcgtggac gcgatctgtg accattacgc cgaacggctc ggattggctg gccgctgacg
      901 cggccggtcc tcgggagcct ctatgggcga taccctgcga gtcggcatcg atgtgggcgg
      961 cacgttcacc cacgccgtgg ccatcgatgc catgacccag agccttcggg catcggtgcg
     1021 cgttcccacg acgcaccggg cgactcgggg tgtggccgag ggtgtctgcg aggcgctccg
     1081 ggcgctgctg gcccggctcg gccagtcccc ggagtctgta gccctgattg cccacagtac
     1141 cacccaggcg accaatgcgc ttctcgaggg tgacgtcgct cctgtgggcg tcctcgccgt
     1201 gtcagtcggc cgatcgggca tgtcgagggt gacctccgcg cgggcggaga gggagaccga
     1261 tgtaggcgac acccccatcg gggagggggg ccaccggtcg gtgccggtcc ggcatgtgca
     1321 tgtcgagtgc accagtgagg agctctctga cgcggtgggg gcggccattg ggaccctcaa
     1381 agccgaggga tgcgaggtca tcgtggccgc cgaagcgttc tcgatcgagg atccctccaa
     1441 cgagagacga gttgccgagc tggcgatcca ggcgggtctg ccggccacgg ccacgtcaga
     1501 catctcgctc ctgcacgggc tgcgccaacg gacccgcacg gctgtcgtga acgccagcat
     1561 cttgcccaag atgtccgagg ccgcacggtg caccgaggac gccgcccggg ccctgggatt
     1621 ccaggctccc ctgctgatcg tccgcagtga cggcggaacc atgtcggcag aggaggccac
     1681 gcgccggccg ctgctggtca acgccacgcc gc
//
LOCUS       DAOSPL010001176         1711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_70454_length_1711_cov_2.626208, whole genome shotgun sequence.
ACCESSION   DAOSPL010001176 DAOSPL010000000
VERSION     DAOSPL010001176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70454_length_1711_cov_2.626208"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1711
                     /locus_tag="PLQ54_17915"
     CDS             <1..>1711
                     /locus_tag="PLQ54_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95197.1"
                     /translation="RAGDLLAEMDRDGYASQGQISELESALSTIHAGPAAVRVLAQAI
                     EAGRPMLVNAAGNAVSRARAAGDPALLLPVEGNELVCKACRGETESLSAVVSALAGLT
                     NLRVVASDLQGPVGTIPASAVDVRIVKWWYQGASGSIGYSPKKALLAELLLKDDALVR
                     VDTETQDNLLRSTQPDGTIEYLVCSAPDSSGLAGVRPVDAATLQPVDLAAGTSREYWV
                     NVTVPTHAASGEYMGTLTFACDQGSAELPLRLTVRPFDLAPSRLTYSIYYRGCLSPDG
                     QPTIGSELKSDAQYFAEIADMKAHGVLYPTNYQGMGDDRVERVLELRAKVGLPRGRFY
                     NLGTGVGPRATPEELEAMVGDLKAWMDLLAPYGYDEVYFYGTDEATGDALTAQKASWR
                     AAQEAGARTFVACYRGTFDAMGSLLNCAVFAGAPDPEEARRWHSVGSEVFCYANPQVG
                     VEDPPLYRRNFGLVLWKAGFDGAMDYAYQHGFGHVWNDFDDRTYRDHNFTYPTVNGVV
                     GTIQWEGFREGVDDVRYVTTLERAIEHHRAICAQIRAGNVEGARQAMREDLAEAESLM
                     RQAL"
BASE COUNT          275 a          574 c          583 g          279 t
ORIGIN      
        1 gcgcgccgga gacctgctag ccgagatgga tcgcgacggc tacgcgtctc agggccagat
       61 cagtgagctg gagtcggcgc tcagcaccat tcacgccggc ccggccgcgg tccgtgtgtt
      121 ggcgcaagcc atcgaggccg gacggcccat gctcgtcaat gccgccggca acgcggtcag
      181 ccgcgcgcgg gccgcgggcg atccggccct cctcctgccc gtggaaggta acgagcttgt
      241 gtgcaaggcc tgccgcggag agaccgagtc gctctccgcg gtcgtgtcgg cgctggccgg
      301 gctcacaaac ctgcgcgtgg tcgcatcgga cttgcagggc cctgtcggga ccatcccagc
      361 ctctgccgtc gatgtccgca tcgtgaagtg gtggtaccag ggcgccagcg ggtccatcgg
      421 ctactcgccg aagaaggcgc tgctggccga gttgctcctc aaagacgatg cgctcgtgcg
      481 ggtcgatacc gagacacagg acaacctgct gcgcagtacg caaccagacg gcaccattga
      541 gtacttggtc tgcagcgccc ccgatagctc gggccttgcc ggcgtccggc cggtcgatgc
      601 cgccacgctt cagccggtcg acctagctgc cgggacgagt cgcgagtact gggtcaatgt
      661 gaccgtgcca acccatgcgg cttcgggcga gtatatgggc acactcacct tcgcgtgcga
      721 ccagggcagt gccgagttgc cgttgcgcct cacggtccgg ccgttcgacc tcgccccatc
      781 tcggctgacc tactccatct actaccgcgg ctgtctctcg ccggacgggc agccgaccat
      841 cgggtcggag ctgaagtccg acgcgcagta cttcgccgag atcgccgaca tgaaggccca
      901 tggcgtgctc tacccgacca actaccaggg catgggcgat gaccgcgtcg agcgagttct
      961 cgagctgcgc gcgaaggtcg gcctgcccag agggaggttc tacaacctgg gcacgggcgt
     1021 cggacccagg gcgacgcccg aggagctcga ggccatggtc ggcgacctga aggcgtggat
     1081 ggacctgctg gccccctatg ggtatgacga ggtctacttc tacggcaccg acgaagccac
     1141 cggtgacgcg ttgacggcgc agaaggcctc gtggcgcgcc gcgcaggagg cgggtgctcg
     1201 caccttcgtg gcctgctacc gcggcacgtt cgatgccatg gggtcactgc tcaactgcgc
     1261 cgtgttcgcc ggggctcccg accctgaaga ggccagacgc tggcactcgg ttggctctga
     1321 ggtgttctgc tacgcgaacc cgcaagtggg cgtcgaggac ccgccgcttt accgccgcaa
     1381 cttcgggctc gtgctgtgga aggcgggctt cgacggcgcc atggactacg cctatcagca
     1441 cggcttcggg catgtctgga acgacttcga cgaccgcacc taccgcgacc acaacttcac
     1501 ctaccccacc gtcaacggcg tggtcggcac cattcagtgg gagggcttcc gcgagggtgt
     1561 cgatgatgtg cgttacgtga cgacgctgga acgcgccatc gagcaccatc gcgccatctg
     1621 cgcgcagatc cgcgcgggca acgtcgaggg ggcgcggcag gcgatgcgcg aggacctggc
     1681 cgaggcggag tccttgatgc gccaggcgtt g
//
LOCUS       DAOSPL010001177         1709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_70556_length_1709_cov_3.202539, whole genome shotgun sequence.
ACCESSION   DAOSPL010001177 DAOSPL010000000
VERSION     DAOSPL010001177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70556_length_1709_cov_3.202539"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLQ54_17920"
     CDS             <1..184
                     /locus_tag="PLQ54_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOS95198.1"
                     /translation="KGLEAAGEVDQAKLRDAIEATQGFVGTAGIFNYSARDHNGLTVD
                     AFVWVKIEDGKWKLAE"
     gene            231..1100
                     /locus_tag="PLQ54_17925"
     CDS             231..1100
                     /locus_tag="PLQ54_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOS95199.1"
                     /translation="MSLADQLVQYVIAGLSGGLVYGLVALGLTVIYNATGVINFAQGE
                     FVMLGGMSAVALNAAGMPLWLSAVLAVAIVTAVGIGLVRLTIYPVRDRPALSVIIVTI
                     GASLVLQGGAKLLWGPDAVRLRSFSGDTPIRLGAGGIEPQRLWVAGLALLTVVAVHGF
                     FTWTAHGKAMRAVSLNRAAARLCGINADSVVALAFGLSAATAALAGVALTPVEMMRCS
                     QGTHYTLAGFLAAVVGGLGNGFGAVVGGLVLGLLEALSKGLISSGYARAIALGVALLV
                     LWLRPQGLLGRRR"
     gene            1104..>1709
                     /locus_tag="PLQ54_17930"
     CDS             1104..>1709
                     /locus_tag="PLQ54_17930"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOS95200.1"
                     /translation="MGTEATPLAEEPTTPRPGIRPAKVAGWALLCAIALAPVVARSGY
                     LSSMGCQIATSAIIVVGLGLLMGYAGQISLGHAAFYAIGAYSSAILTTKAGVHPLLAL
                     CAGAAASALLALIVGTPALRLHGHYLAMFTLGLGIIVQIAIEQGKRLTNGFDGITGIP
                     ALDLGFICLDSDPRMYGATVVALVGCVLAARNLVDSRPGRAL"
BASE COUNT          224 a          599 c          583 g          303 t
ORIGIN      
        1 caagggtctc gaagccgcgg gtgaggtcga ccaagccaag ctgcgtgatg ccatcgaggc
       61 gactcaggga ttcgtgggca ccgccggcat cttcaactac agtgccagag accacaacgg
      121 gctgactgtc gatgccttcg tgtgggtgaa gatcgaggac ggcaagtgga agctggcgga
      181 gtagcccggg cgaggcgcgt aggggcaccg gtggcgggac tccatcgccc atgagcctcg
      241 cggaccagct cgtccagtat gtgatcgcgg gcctctccgg gggcctggtc tacggcctgg
      301 tggccctggg cctcacggtc atctacaacg ccaccggggt catcaacttc gcccagggcg
      361 agttcgtgat gctcggcggg atgtcggccg tggcgctgaa cgcggccggc atgccgctat
      421 ggctctccgc cgtgctcgcc gtggccatcg tcaccgccgt gggtatcggc ctggtccggc
      481 tgaccatcta ccccgtgcgg gatcggcccg ctctgagtgt catcatcgtg accatcggcg
      541 cctctctggt gcttcagggg ggcgccaagc tgctctgggg cccggacgcg gtccggctgc
      601 ggtccttcag cggcgatacg cccatccggc tcggagccgg cggcatcgag ccccagcggc
      661 tgtgggtcgc cggcttagct ctgctcacgg tcgtggccgt ccacgggttc ttcacctgga
      721 ccgcccacgg caaggccatg cgcgccgttt ccctcaaccg tgcggcggcg cgactgtgtg
      781 gcatcaacgc cgactccgtc gtcgctctgg ccttcgggct cagcgcggcc acggccgctc
      841 tggcgggagt cgccctgacc cccgtcgaga tgatgcgctg ctcgcaaggc actcactaca
      901 cgctggccgg cttcctcgcc gccgtagtcg gcgggctcgg caacggcttc ggtgccgtgg
      961 tgggcggcct ggtcctgggt ctgctcgaag ccctctcgaa gggtctgatc tcgtcgggct
     1021 acgcccgggc catcgccctg ggcgtggccc tgctggtcct ctggctccgc ccacagggcc
     1081 tgttgggccg acggaggtag tctgtgggaa ccgaggcaac gccgctggca gaagaaccaa
     1141 caactcccag gcccggcatc cggcccgcga aagtcgccgg ttgggccctg ctgtgcgcca
     1201 tcgccctggc gccggttgtg gcccgcagcg gctatctctc gagcatgggg tgtcagatcg
     1261 ccaccagcgc catcatagtg gtcggcctgg ggctgctgat gggctacgcg gggcagatct
     1321 cgctcggcca cgcggccttc tacgccatcg gtgcctactc cagcgccatc ctgaccacca
     1381 aggcgggggt gcaccccctc ctggctctgt gcgccggtgc tgcggcttcc gcgctgctgg
     1441 ccctgatcgt gggtacgccg gcacttcggc tccatggcca ctacctggcc atgttcacac
     1501 tgggcctcgg gatcatcgtc cagatcgcca tcgagcaagg gaagcggctc acgaacggat
     1561 tcgacggcat caccggcatc cccgcgctcg acctgggctt catctgcctc gactccgacc
     1621 cgcgcatgta cggcgccacg gtggtggccc tggtcggctg cgtcctggca gcgcggaatc
     1681 tggtcgactc ccgtcccggc cgagcgctg
//
LOCUS       DAOSPL010001178         1709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_70576_length_1709_cov_2.261185, whole genome shotgun sequence.
ACCESSION   DAOSPL010001178 DAOSPL010000000
VERSION     DAOSPL010001178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70576_length_1709_cov_2.261185"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1709
                     /locus_tag="PLQ54_17935"
     CDS             <1..>1709
                     /locus_tag="PLQ54_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95201.1"
                     /translation="NWWAGQVYAPNLSITECRSALLSQTEEAYGDLLDEGVLSGVFYD
                     SVVGGASWYGEVDADRDGIADAPADIDPRWHGYQCAFFDDLRARHPAMAIMANDVDLG
                     HAAHLHGRLFEGGPLLDRVANGSIGPRDAVRVLQEWMRASLQPGITFAIMTHPMGWQG
                     WRVGKGNAVTTPGEVDRVRRDFARMRLGLLTTLMTDAYFAYDMGTVWYGLPYWYDEYT
                     SSLGQPLGSCQEVFDVPPVTVLDWSAGQALGGIIADASASETPEGLEARCTDPAAGWQ
                     RTIGTRPSDVRLEPGKRYRIQADVDILERPTEALQFCVRTAKGGWEHHDKGVQTCATA
                     AGATWHIETTIVPDDFDDYSIEWHLRGAGAFRLTHLSVHSISESYLMREFEGGFAILN
                     TSTRPLPVRLPRPVRRLRGDQAPLHIIELDDSSGAFDPQGAWERVAGERHYAGAGFRT
                     ARKPGDTAAWLFRAPAADRYTVFACIPGGADLSDAAVYRLDGAESSPSATVDQRPCDG
                     GWVRLFDADLAPDHTYSVTLQSGGAGLTAADAIRLESASRYNDGARVVDEWLAPLDGR
                     ILL"
BASE COUNT          274 a          577 c          577 g          281 t
ORIGIN      
        1 caactggtgg gcgggccagg tctatgcacc gaacctgtcg atcaccgagt gccgctcggc
       61 cctgttgtcc cagaccgaag aggcctacgg cgatctgcta gatgagggcg tgctcagtgg
      121 tgttttctac gacagcgtcg tcggaggcgc cagctggtat ggcgaggtcg atgccgaccg
      181 agatggaatc gccgatgctc cggcggatat cgatccccgc tggcatggct atcagtgcgc
      241 cttcttcgat gacctcaggg cgaggcaccc cgccatggcc atcatggcca acgacgtcga
      301 cctgggccac gcggcgcacc tgcacggacg gctgttcgag ggcggtccac tgctcgatcg
      361 agtcgccaac gggtccattg ggcccaggga cgcggtccgt gtgctgcagg agtggatgag
      421 ggcctccctt cagcccggaa tcactttcgc catcatgacc caccccatgg gctggcaggg
      481 ctggcgcgtc ggcaagggca acgcggtaac gacgcccggc gaggtcgacc gtgtccggcg
      541 cgatttcgcc cgcatgcgcc tgggcctgct caccactctc atgaccgatg cgtacttcgc
      601 atacgacatg ggaacggtat ggtatggcct tccgtactgg tatgatgagt ataccagctc
      661 gctgggacag cccctgggca gctgccaaga ggtctttgat gtcccacccg tgaccgtcct
      721 cgactggtcg gctgggcagg cgctgggcgg gatcatcgcc gacgcatcgg cctcagagac
      781 gcccgagggc ctggaagccc ggtgcaccga tccggcggcc ggctggcagc gcaccatcgg
      841 gacgcggccc tccgatgtcc ggctcgagcc cggcaagcgg taccgcatcc aggccgatgt
      901 cgacatcctg gagcggccca ccgaggccct ccagttctgc gttcgcaccg ccaaaggtgg
      961 gtgggagcac cacgacaagg gcgtgcagac ctgcgccacg gcggcgggcg caacctggca
     1021 catcgagacg accatcgtgc ccgatgactt tgacgactac tcgatcgagt ggcacctgag
     1081 aggggctggc gcgttccgcc tgacacatct aagtgtacac tcgatcagcg agagctacct
     1141 catgcgtgag tttgaaggcg gctttgcaat actgaacacc agcacgcgcc cgctgccggt
     1201 tcggctcccc cgccccgtgc gcaggctgcg aggcgaccag gccccgctcc acatcatcga
     1261 gctcgatgac tcctccggtg cctttgaccc acagggcgcc tgggagcggg tcgcggggga
     1321 gcggcactac gccggggcgg ggttccgaac tgcccggaag cccggagaca cggccgcgtg
     1381 gctctttcga gcgccggccg cagaccgcta caccgtcttc gcctgcatcc cgggcggggc
     1441 cgacctctcc gacgcggcgg tctatcgcct cgatggcgct gagagctcgc cctcggccac
     1501 cgtcgaccag cggccgtgtg atggcgggtg ggtgcggctt ttcgacgccg atctggcgcc
     1561 ggaccacacc tattcggtga cgctgcagag tgggggcgcg ggcctcacgg ctgccgacgc
     1621 catccggctg gaaagtgcct cccgctacaa cgacggagcc cgggtggtcg acgagtggct
     1681 ggcgcccctc gatggccgga tactgctga
//
LOCUS       DAOSPL010001179         1708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_70606_length_1708_cov_3.595886, whole genome shotgun sequence.
ACCESSION   DAOSPL010001179 DAOSPL010000000
VERSION     DAOSPL010001179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70606_length_1708_cov_3.595886"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..664)
                     /locus_tag="PLQ54_17940"
     CDS             complement(158..664)
                     /locus_tag="PLQ54_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95202.1"
                     /translation="MATAALQSPTPPPALTTEGSPRYLDPRDQFIRSPQPISLTRLAG
                     QWHQPGRRDGHSLMALYRRSKQEGWEQCRAAYHTRRKESSEAETAHAADSPAVLWARY
                     VRYRLPQILQALVEQAEKGNLTAIKIILEAEGLTEKSGLLDAEDNYPRIVAECCLLAD
                     ERLPEEQR"
     gene            complement(889..1131)
                     /locus_tag="PLQ54_17945"
     CDS             complement(889..1131)
                     /locus_tag="PLQ54_17945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95203.1"
                     /translation="MAASWFRCTGTVVSTGFSIARLVGRLLVMLLVTRASRWRAVRAF
                     RRGLVDAGLPPEAIRELTRAFPHLPGLAAVSRRWAR"
     gene            complement(1134..1415)
                     /locus_tag="PLQ54_17950"
     CDS             complement(1134..1415)
                     /locus_tag="PLQ54_17950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95204.1"
                     /translation="MSEHGHSDAAEVREILDVVADKIPALLNQLRNTLFSAEAGQEIG
                     RAVGAFYRELVESGIPADEALAMAKSYIGSLEGVLNQAKMGGHHGHKEG"
     gene            complement(1434..>1708)
                     /locus_tag="PLQ54_17955"
     CDS             complement(1434..>1708)
                     /locus_tag="PLQ54_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95205.1"
                     /translation="AERRQVFAHIAVCATCRREFVEVVALARRVESATEALPGPARTV
                     DCLALLEAAGLPLLVVELVRGARSLVRQGVVVSLGLPWVAPLTVRV"
BASE COUNT          295 a          580 c          562 g          271 t
ORIGIN      
        1 ttctccgtgc gcaacctgct cacgaccggc cgcgttccgc gggacgcacc ggcggcaacg
       61 cctcagtccg tcgaagaccc ggccataccg cctgccgact ggcccccccc gcctgccgag
      121 gacctctggt gagaagggcc ggacgggcga gcgaaaccta gcgctgctcc tcgggaagcc
      181 tctcatccgc caggaggcag cactcggcca cgatccttgg atagttgtcc tcggcgtcca
      241 acaggccgct tttctcggtg agcccctcgg cctcgaggat gatcttgatg gcggtgaggt
      301 ttcccttctc ggcctgttcc accagagctt gtaggatctg gggcagccga tagcgcacat
      361 atcgtgccca cagaacggcc ggggagtccg cggcatgcgc ggtctctgcc tccgaagact
      421 ccttccgccg tgtgtggtag gccgcgcggc actgctccca gccctcttgc ttggatcgcc
      481 gatagagcgc catgagggag tgtccatcgc gccggcccgg ctggtgccac tgcccggcca
      541 gccgggtgag ggagatgggt tggggggacc ggatgaactg atcccggggg tccaggtacc
      601 gcggagaccc ttcggtggtc agggcagggg gtggggtagg ggactgcagg gcagcagtgg
      661 ccatggcgac ctccaaacag atgtttgtat atgtcgccat catagcacat ttgtttgcat
      721 atgtcaatgg gccccgtgac gcgaagcagg ccacgtccag ggctcggagt ccacgaaggg
      781 cacgaaggaa ccacagggtc gggcgtccac acagcgatgg gaatcggaga ccgatcattc
      841 gtccccgatt cccggcgatg aggtaggcgc cagtgcgctc gttgcctcct accttgccca
      901 ccggcgagag acggccgcga gccccgggag atgcgggaag gctcgggtca gctcgcggat
      961 cgcctccgga ggcaggccgg catcgacgag acctcggcgg aatgcccgca ccgctcgcca
     1021 gcgggaagct cgggtcacca gcagcatcac caaaagacgg ccgacgagcc gcgcgatgct
     1081 gaagcccgtg ctcacgaccg tgccggtgca tcggaaccac gacgccgcca cggctagcct
     1141 tccttatgtc cgtggtgccc gcccatcttc gcctggttca gcacgccctc cagggatccg
     1201 atgtagctct tggccatcgc cagggcctcg tcggccggga tgccgctttc gaccaattcc
     1261 cggtagaagg ctcccacggc gcggccaatc tcttgcccgg cctcggcgga gaacagggtg
     1321 ttccggagct gattgagcag ggccgggatc ttgtcggcaa ctacatcgag gatctcgcgg
     1381 acctctgcgg cgtcagaatg accgtgttcg gacatgttcg gcctctcttt cccctacaca
     1441 cgaaccgtca atggtgcgac ccagggcaag cccaaagaga ccacaacgcc ctggcgtacc
     1501 aggctgcggg cccctcggac cagctcaacc accaggagcg gcaaaccggc cgcctccagc
     1561 agggcaaggc aatccaccgt ccgggcggga ccgggtaggg cttcggtggc cgactcgacc
     1621 cgccgcgcga gagcgaccac ttcaacgaac tcgcgccggc aggtcgcgca gacggcgatg
     1681 tgcgcgaaca cctgccgccg ctcggcgg
//
LOCUS       DAOSPL010001180         1708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_70612_length_1708_cov_3.081670, whole genome shotgun sequence.
ACCESSION   DAOSPL010001180 DAOSPL010000000
VERSION     DAOSPL010001180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70612_length_1708_cov_3.081670"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1708)
                     /locus_tag="PLQ54_17960"
     CDS             complement(<1..>1708)
                     /locus_tag="PLQ54_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95206.1"
                     /translation="DPLVPQPLADQTFVYGGTAFTDPTVVSLPLVTLLDPAAGRGLSI
                     VQSPEDTLRRMTIAISRQGEVTFCHLDHRISAAAPVRLSIDLVVHEDDWRPALGWLAR
                     RYPAFVDPPNPRAHDVAGLGAYSSEEGDLDAARLAAMGFGVNWKASFDFPYMGMFLAP
                     VPAWVEWTSFLGRATSVASLRDYSRRMRQQGFRVLNYFNVTEFGTRVVDPPPPRKAGT
                     DADLWRDANDFLYHGPLRDAILPGPEGKPIFTWEAGIAMDPGDPAYRDFLLAQADRHI
                     RQIPHSDGICIDRLDWLARYNPRFDDGVTWVDGGPARSLFASWAELMARLGPMMHAAG
                     KVIFVNPHTRRLDLMRHVDGIYDEFGDMPHSLNACGLLGFRKPVMGWTASTASLQPDP
                     DAVFQRHLHMGVHPTAPLPGNDHTIRPDPFTDPYYLDYGPLFDALRGRRWVTATHAIE
                     VEGAVAKANVFAIAGGYAAPVTFGGTATSARVRLRNLTWPGPWSAASIDVLVPGRTAP
                     VQVAVTRRGDTLAVDVPLVRGCGLLRLRHTWVTPRPRVFITQTSVAMGTGIAGATLRY
                     TLD"
BASE COUNT          305 a          517 c          636 g          250 t
ORIGIN      
        1 gtcgagcgtg tagcgaagag tcgcaccggc gatgccggta cccatggcga ccgatgtctg
       61 tgtgatgaag acacggggcc gtggcgtgac ccaggtgtgg cgtagccgga ggagaccgca
      121 gccgcgaacc aggggcacat ccaccgcgag cgtgtcaccg cggcgggtga cggcgacctg
      181 cacgggtgcc gtcctgccgg ggacgagaac gtcgatgctc gccgccgacc acgggccggg
      241 ccaggtcagg ttccgcagac ggactcttgc agacgtcgcg gtaccaccga aggtgacggg
      301 cgcggcgtag ccgccggcga tcgcgaagac gttcgccttc gccacagcgc cttccacctc
      361 gatggcgtga gtggccgtga cccacctacg gccccgcagc gcatcgaaca gtgggccata
      421 gtcgaggtag tacgggtcag tgaacggatc aggccggatc gtgtggtcat tgcccggcag
      481 cggggccgtg gggtggacgc ccatgtgcag gtgccgctgg aacacagcat cggggtcggg
      541 ctgcaaagag gcggtggagg cggtccagcc catcacaggc ttccggaagc caagtaggcc
      601 acaggcgttg aggctgtgtg gcatgtcgcc gaactcatca tagatgccgt ccacatggcg
      661 catgaggtcg agccggcgcg tgtgcgggtt cacgaagatg accttgccgg ccgcgtgcat
      721 catgggtccg agcctggcca tcagctcggc ccaactggcg aacagcgatc gtgcaggccc
      781 cccgtcgacc caggtcacgc cgtcgtcgaa gcgcgggttg taccgggcga gccaatccag
      841 gcggtcgata cagatgccat cgctatgggg gatctggcgg atgtggcgat ccgcctgggc
      901 cagcaggaag tcgcgatagg ccgggtcgcc ggggtccatg gcgatgccgg cttcccaggt
      961 gaatatgggt ttaccttcgg ggcccggcag gatggcatcg cgcagcgggc cgtggtagag
     1021 gaagtcgttg gcgtcgcgcc acaggtcagc gtcggtaccg gctttgcggg gtgggggcgg
     1081 atcgacaaca cgcgtgccga actcggtgac gttgaagtag ttgagcacgc ggaagccttg
     1141 ctggcgcatc cggcgtgagt agtcccgaag gctggcgacc gaggtagccc tgcccaggaa
     1201 tgaggtccac tccacccagg cgggcacggg ggcgaggaac atgcccatgt aggggaagtc
     1261 gaagctcgcc ttccagttga caccaaagcc catggcggcc agacgcgcgg cgtcgaggtc
     1321 gccttcctcc gacgagtagg ccccaaggcc cgccacatcg tgagcgcggg ggttcggcgg
     1381 gtccacgaaa gcgggatagc gccgggcgag ccagcccaga gccggccgcc aatcatcctc
     1441 gtgcacgacg aggtcgatcg agaggcgtac cggcgcggcg gccgagatgc ggtgatccag
     1501 atggcagaac gtcacctcgc cctgtcttga aatggcaatc gtcatgcggc ggagggtgtc
     1561 ttcaggcgac tggacgatgc tgagcccgcg accggcagcg gggtccagca gcgtgacaag
     1621 cgggagcgag acgacggtgg ggtcggtgaa agccgtgccg ccgtacacga atgtctgatc
     1681 ggccagaggc tgcggcacca gcgggtcc
//
LOCUS       DAOSPL010001181         1707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_70658_length_1707_cov_4.213680, whole genome shotgun sequence.
ACCESSION   DAOSPL010001181 DAOSPL010000000
VERSION     DAOSPL010001181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70658_length_1707_cov_4.213680"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PLQ54_17965"
     CDS             <1..423
                     /locus_tag="PLQ54_17965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95207.1"
                     /translation="TPDGLAALARYGVRVLSGYWEPSPWGYDVSYWTDDAKSDYLHRH
                     DCMKDFATGIVFSRIDIVINNTPIDQIDGRLDPLVDDPNHAEIMDLLTHEQYFWDFYA
                     NYIPDHPERLERAIRWVTDHGYKPVFFHQGFLGAPDGE"
     gene            complement(465..1529)
                     /gene="pheA"
                     /locus_tag="PLQ54_17970"
     CDS             complement(465..1529)
                     /gene="pheA"
                     /locus_tag="PLQ54_17970"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOS95208.1"
                     /translation="MDLEGPRKRIDEIDAEIARLLSERGELAVEVGRIKAQHSVRVYN
                     PQREMQVLENVFRHASGPLGREAIERIYREVISACRALEDRPVIAHFGAPASFTHMAA
                     VRSFGSDCDFVALDDMPDVFTTVEKGAAQYGVVPVENSTGGVVPQTIDRFIESPLRIV
                     GERYMDVHHYLCSKGTLEEIQRVYTHPQVFAQCQRWLREHLSGSERYEESSSSRGAER
                     ASQEGPGAAAIATPLAAEIYGLDILAERIEDYTLNRTRFWIIGKDENPPTGRDKTSIL
                     FTTRHVAGSLIRALEVFDRHQINLTMIQSRPIKGAAWEYVFFIDLQGHPADNIVAAAL
                     DELDKVTLFYRVLGAYREEE"
BASE COUNT          272 a          536 c          585 g          314 t
ORIGIN      
        1 acacccgacg gcctggcggc gctggcgcgc tatggtgtga gggtgctctc gggctactgg
       61 gagccctcgc catggggcta tgacgtgagc tactggaccg atgacgcgaa gtcggactac
      121 ctgcaccgcc acgactgcat gaaggacttc gcgacaggca tcgtgttctc gcggatcgac
      181 atcgtcatca acaacacgcc gatcgaccag atcgacggcc gcctggatcc gctggttgac
      241 gatccgaacc atgcggagat catggacttg ctgacgcacg agcagtactt ctgggacttc
      301 tacgccaact acatccctga ccacccggag cggctcgagc gggccattcg ctgggtgacc
      361 gatcatggct acaagccggt gttcttccac cagggtttcc tcggtgcccc ggacggggaa
      421 tgagcggcgc ccgaggccgg ctgggcggcg ccttcgtcgg cgtcctactc ctcctcgcgg
      481 taggcaccga ggacgcggta gaacagcgtc accttgtcga gctcgtccaa ggcggcggcc
      541 acgatgttgt ccgccgggtg gccctggagg tcgatgaaga acacgtactc ccaggcggcg
      601 cccttgatgg gccgggactg gatcatggtc aggttgatct ggtgccggtc gaacacctcg
      661 agggctcgaa tcagtgatcc ggccacgtgg cgtgtggtga agaggatgga ggtcttgtcg
      721 cggccggtgg gcgggttctc atccttgccg atgatccaga accgtgtgcg gttgagggtg
      781 tagtcctcga tgcgttcggc caggatgtcg aggccgtaga tctcggcagc caggggcgta
      841 gcgatggcag cggcgcctgg cccttcctgg ctagcacgct cggccccccg cgacgaggag
      901 ctctcctcat atcgttccga cccgctcaga tgctcccgca accacctttg gcactgggcg
      961 aacacctgag ggtgggtgta gacgcgctgg atctcctcga gcgtgccctt cgagcacagg
     1021 tagtggtgga catccatgta gcgctcgccg acgatgcgca atggggattc gatgaaacga
     1081 tcgatggtct ggggcaccac gcccccggtc gagttctcga cgggcacgac accatactgg
     1141 gccgccccct tctcgacggt tgtgaagacg tctggcatgt cgtcgagggc gacgaagtcg
     1201 cagtcggacc cgaagctgcg gacagcggcc atgtgggtga agctggcggg cgcgccgaag
     1261 tgggcgatga ccgggcgatc ctcgagtgcc cggcaggcgg agatgacctc gcggtagatc
     1321 cgctcgatgg cctcccggcc cagcgggccc gacgcgtgcc ggaacacgtt ctcgagtacc
     1381 tgcatctcgc gctgggggtt gtagacgcgt acggagtgct gggccttgat gcggcccacc
     1441 tcgacagcca gctcgccgcg ctcgctcagc agacgggcga tctctgcgtc gatctcgtcg
     1501 atccgcttcc gcgggccttc caggtccatg cgttccgcct ttgtgaggaa acggggtcag
     1561 ggtgcgtttc cgctgcgtcg cgcgttcttc gacgcaggtg ggcgcttccc ctccggtgaa
     1621 caggggcgac cgggtaagac tcggggcgcc cggggagcac cgatttgccc gcaggttgtg
     1681 gccagcgatc cgcgattcct cttcgcc
//
LOCUS       DAOSPL010001182         1707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_70669_length_1707_cov_3.003632, whole genome shotgun sequence.
ACCESSION   DAOSPL010001182 DAOSPL010000000
VERSION     DAOSPL010001182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70669_length_1707_cov_3.003632"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PLQ54_17975"
     CDS             <1..614
                     /locus_tag="PLQ54_17975"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOS95209.1"
                     /translation="AVILGGFAAGEAGPVRLLGIRPMVWIGGLSYSLYLWHWPLVVAA
                     TGLWGKLSDWQGAAVVTASLVPAYLTHRFVENPLRYHKAVSARSAVALGLGAACSLIG
                     VGAGVWLHHAGGTSGFSTASSRNAVGAGSLGTGATPTSAPSIDMHPKAMTPDPLKATQ
                     DVPQLYADGCQVAQPSTAVISCTYGDRSSRKIIAMVGDSKMGQ"
     gene            754..1551
                     /locus_tag="PLQ54_17980"
     CDS             754..1551
                     /locus_tag="PLQ54_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HOS95210.1"
                     /translation="MLSYRATLDIPLTTVARVSRWLQAHRRRVDTRPWQRAATCWTQA
                     ILVLRWLKDGTDIDLLARDAKVSQATGYRYLHEAIGVIAAQLPDLPEVLANGHAAGWA
                     YVCLDGTLISSTRSSAKSDAGHDLWYSGKHKRHGGNIQVVTDPSGFPVWVSPVEPGST
                     HDLSAARLHVLPALYPAAAAGMPTLADKGYVGAGIGIHVPFKGANLCPDNRTRNLLIS
                     ALRAPAERGNALLKSVKALRRVTLCPWRIGDITAAALVILTMHRGRW"
BASE COUNT          278 a          633 c          532 g          264 t
ORIGIN      
        1 ccgccgtcat cctcggggga ttcgcggccg gcgaggcggg cccggtgcgg ctgctcggca
       61 tacgccccat ggtgtggatc gggggactgt cgtattcgct ctatctctgg cactggccac
      121 tcgtcgtcgc cgcgaccgga ctatggggga agttgtccga ctggcagggc gccgccgtcg
      181 tcaccgcgag cctcgtcccc gcctatctga cccaccggtt cgtcgaaaac ccgctccgct
      241 accacaaggc ggtctcggcg cgctccgcgg tcgcgctcgg cctcggcgcg gcgtgctcgc
      301 tgatcggggt cggcgcgggg gtgtggctgc atcatgcggg cggcacgagc gggttctcca
      361 ccgcctcctc caggaatgcc gtcggcgccg gcagtctcgg gaccggcgcg accccgacgt
      421 cggcaccgag catcgacatg caccccaagg cgatgacccc ggatccgctg aaggcaaccc
      481 aggacgtccc tcagctctat gccgacggtt gccaggtcgc gcagccgtcg accgccgtca
      541 tctcgtgcac gtacggcgac aggtccagcc gcaagatcat cgccatggtc ggggattcca
      601 agatgggcca gtgaggtttt ctcaccaggc ctggggcttg gcgagtggtg tgacgcgtgt
      661 gacctggggt gatggcgtgt cggatggtgc acgcggggtc cggggcgcca gagtcggttt
      721 cgacgaagtc cccttctcga gccaggaccc cgcgtgctgt catatcgtgc caccctcgac
      781 atccccctga ccaccgtcgc ccgggtctcc cgctggctac aagcccaccg ccgccgagtc
      841 gatacccggc catggcaacg agccgcgacc tgctggaccc aggccatcct ggtcctacgc
      901 tggctcaaag acggcaccga catcgaccta ctcgcccgcg acgcgaaggt cagccaagca
      961 accgggtacc gctacctcca tgaggcgatc ggcgtgatcg ccgcccaact ccccgacctg
     1021 cccgaggtac tggccaacgg acacgccgcc ggatgggcat acgtgtgcct ggacggcacc
     1081 ctgatcagct ccacccgctc atcggcgaaa tccgatgcgg gacacgacct gtggtactcc
     1141 gggaaacaca aacgccacgg cgggaacatc caggtcgtca ccgaccccag tgggttcccg
     1201 gtctgggtct ccccggtcga acccggctcc acccacgacc tgagcgccgc ccgcctgcac
     1261 gtgctgcccg ccctctaccc ggccgccgcc gccggcatgc cgaccctggc cgacaagggc
     1321 tacgtcggcg ccggaatcgg gatccacgtc ccattcaagg gcgccaacct ctgccccgac
     1381 aaccggacgc ggaacctcct gatcagcgct ctccgcgcgc ccgccgaacg cggcaacgct
     1441 ctgctgaaat ccgtcaaggc cctgcgccgc gtcactctct gcccctggcg catcggcgac
     1501 atcaccgccg ccgcgctcgt catcctcacg atgcaccgag gccgctggtg agaaaacctc
     1561 agtcactggc caatggcacg ggtagcggag cggagtcccc aagcatcacg aagaagtagt
     1621 actcgtcagc acgcctgaac tccgagcctc gcgcaatcgc agccgggttg attgcgacag
     1681 agatcatctg tatccgagct tcgggga
//
LOCUS       DAOSPL010001183         1705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_70790_length_1705_cov_1.929697, whole genome shotgun sequence.
ACCESSION   DAOSPL010001183 DAOSPL010000000
VERSION     DAOSPL010001183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70790_length_1705_cov_1.929697"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            374..535
                     /locus_tag="PLQ54_17985"
     CDS             374..535
                     /locus_tag="PLQ54_17985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95211.1"
                     /translation="MRGRRCTSFAEVPLTLLRANQRRATKALGLEKRPTETIRVSEDA
                     FTGMLGNGA"
     gene            complement(875..1306)
                     /locus_tag="PLQ54_17990"
     CDS             complement(875..1306)
                     /locus_tag="PLQ54_17990"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOS95212.1"
                     /translation="MPRGHCHRHGAQFGPCSCSLGRLSRLVEPAILYLLASGQARYGY
                     ELLDLLREEAMTDSEIDAGVVYRTLQQVERAGCVVSDWEPGHGGPKRHVYRITELGRT
                     HLADWTTVLQRRAEQMLSFVERCRPLLAEPGIAATGPDERS"
     gene            1465..>1705
                     /locus_tag="PLQ54_17995"
     CDS             1465..>1705
                     /locus_tag="PLQ54_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS95213.1"
                     /translation="MIRFSGGITVRRTGFTLIELLVVIAIIAILVAILFPVFAQAREK
                     GRTVSCLSNLKQWGLAATMYAGDYDEMFVPHCLRDF"
BASE COUNT          294 a          569 c          564 g          278 t
ORIGIN      
        1 gggagggtgc gcgtgaacgc ccggggcgcg gccacgcatc ctcccctcct ccttgccacc
       61 cggaacgttt cgagacccgg cagagaagtg agctggagca tgtgagcggc tctgctgcgt
      121 ggtcgcggcc actgcggatg acaagcccgg gtcggggcgc gcccaaggcc atcgtgtccg
      181 cgccgtcccg ggacggcgac tcggccagct gggtcgaggc catcgacgca ttcgtggccg
      241 agcacgaggc ggagaaggcc cgtgccgtcg tcgggttcgc gaaaaagcag gttagccggc
      301 gagccaggtc caggcactcg ccagggagca cgataccgag gtccctctga ccttcccctc
      361 ggatggcccg accgtgaggg gccgaaggtg cacaagcttc gcggaggtgc ccctgacgct
      421 gctgcgtgcc aaccagcgca gagccaccaa ggccttgggc ctcgagaagc ggcccactga
      481 gacgatccgc gtttcggagg acgccttcac gggtatgctg ggcaatggag cgtagcgcat
      541 ggggttgggc ggccgcggga tgacgcatct cccgcggccg ccgttcgcct ggccaccgcg
      601 gagatgcacg cggcccgctc ctaccctcca acccccgccc tgcgtctgcc cgggtcatgc
      661 ccacagccgt ctggttcccc ccatggcaac gcgccacaac caggcccaag ggcccgcggg
      721 agtcccaagt caccgccaag gcctgaaggc ggtcaccgat cgaacgttca ttgccctcac
      781 gtgaccgtcc atggacgcca cgttcgtgaa gctccacttg ggaatccggt gcaggaacgg
      841 cgcgttctgg ccgggcacgc accacggggc ccgtctagct ccgctcgtcc ggcccagtgg
      901 ccgcgatgcc gggctccgcc agcagggggc ggcaccgctc aacgaaggac agcatctgct
      961 ctgcgcgtcg ctggagcacg gtggtccagt ccgccaagtg ggtccggccc agctccgtga
     1021 tccggtacac gtgccgcttg ggccccccgt ggccgggctc ccagtccgag accacacacc
     1081 cggcgcgctc cacctgctgg agggtgcggt agacaacgcc ggcgtcgatc tcagaatcgg
     1141 tcatcgcttc ctcgcgaagc agatcgagaa gctcataccc gtagcgggcc tggcccgagg
     1201 ccaggaggta caaaatggcg ggctcgacca ggcgagagag cctcccgagc gagcaggagc
     1261 agggcccgaa ctgggcgccg tgtcgatggc agtgtccacg aggcatggtc acgtctccac
     1321 tagatgagta ctaagtctgc ttctgcgttg gcctgtcagg ctccggttgg gagatgcgcc
     1381 aacggcaggt gccaaatgca ttgactgact cgcaatgata cgctatgatc cacggtgatc
     1441 cgcttcagta ctagatggag cgccatgatc cgattctctg gagggatcac cgtgaggaga
     1501 acgggcttca ccctcatcga gttgcttgtc gtcattgcca ttatcgccat tttggtcgcc
     1561 atcctgttcc cggtgtttgc ccaggccagg gagaagggac gcaccgtgtc ctgcttgagc
     1621 aacctcaaac agtgggggct cgcagcgacg atgtacgcgg gagactatga cgagatgttc
     1681 gtaccccact gcttgcgtga cttcg
//
LOCUS       DAOSPL010001184         1704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_70813_length_1704_cov_4.346270, whole genome shotgun sequence.
ACCESSION   DAOSPL010001184 DAOSPL010000000
VERSION     DAOSPL010001184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70813_length_1704_cov_4.346270"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..138
                     /locus_tag="PLQ54_18000"
     CDS             1..138
                     /locus_tag="PLQ54_18000"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOS95214.1"
                     /translation="MLADLGSKNGTLLNGRPLEAPTELLDGDRIRIGSTVLEYRAQHP
                     L"
     gene            175..954
                     /gene="cdaA"
                     /locus_tag="PLQ54_18005"
     CDS             175..954
                     /gene="cdaA"
                     /locus_tag="PLQ54_18005"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOS95215.1"
                     /translation="MVDSAGRIDAVAVVDILLLTCLIYQIWCMIEGTRGRQLTQGVFV
                     LILVYLVSRPLPVLHYVLGTAMQPAVIALVILFQPELRSALEQIGRIRPGRSRPGQAV
                     LEALFDAIEHSSVNHIGALIAIEGTTGLSDIAATGTILDAQLTPELLSSVFFPNSALH
                     DGGVILRGDRVLAAGCYFPPTTNPDVPRTLGMRHRAALGMSEHADAVVIVVSEETGGI
                     SLAVSGQLSRNLRMPELRERFRQLFSTEWTLNLAQLWRRRT"
     gene            951..>1704
                     /locus_tag="PLQ54_18010"
     CDS             951..>1704
                     /locus_tag="PLQ54_18010"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOS95216.1"
                     /translation="MKPWRLITHNWSHKLASLAAALLIWVVTVNAQNPIESLEVDVPV
                     EVACGPDYAVRVEQGITTVHAVLRGERALLQPFRDGRRKLVATVRLPEPLEEGRGQGE
                     AMLAPLPGRVRVVSLTPATLAYRLIRLDRKIVRVQTELRGAPIEGVDFGGLVCQPPEV
                     EVVGPKSVVDAVDSVRTIVRRIDLVNQDTVESKVRPIDAQGRTLDDPALTVMRPTVVV
                     SLPPVEPKAVKVSVTTTGHLPDGYQITGIRVSP"
BASE COUNT          277 a          574 c          556 g          297 t
ORIGIN      
        1 gtgctggccg acctgggcag caagaacggc acgttgctga atgggcgccc actcgaggcc
       61 ccgaccgagt tgctggacgg cgaccgaatc cggatcgggt ccaccgttct cgagtaccgg
      121 gcccagcacc cactctgacg aaaggggtgg cgaatggaag cttggtggcg gatcatggtc
      181 gatagcgccg ggcgcatcga tgccgtcgcc gtcgtggaca tcctgctgct cacgtgcctg
      241 atctatcaga tctggtgcat gatcgaggga acgcggggcc gccagcttac ccagggcgtg
      301 ttcgtcctga tactggtcta cctcgtcagc cggcctcttc cggtcctgca ctacgtactc
      361 ggcacggcca tgcagccggc ggtgatcgcc ctggtcatcc tcttccagcc cgagctgcgg
      421 agcgccctgg agcagatcgg ccgcatccgc ccgggccgca gccggccggg gcaggccgta
      481 ctcgaagccc tcttcgacgc cattgaacac tcctcggtga accatatcgg cgccctcatc
      541 gccatcgagg ggaccacggg cctttcggac atcgccgcca ccgggaccat cttggatgcc
      601 cagctgacgc cggaacttct cagcagtgtc ttctttccga acagtgcgct gcacgatggc
      661 ggcgtcatcc tccgcggtga ccgcgtcctg gcggcgggat gctatttccc gccgaccacc
      721 aatcccgatg tgccgcgcac cctgggcatg cggcaccgtg ccgccctggg catgtctgag
      781 catgccgacg ccgtggtcat cgtggtttcc gaggagaccg gaggcatctc cctggccgtg
      841 agtggtcagc tgagccgcaa cctccgcatg cccgagctgc gggagcggtt ccggcagctc
      901 ttctcgacgg agtggactct gaacctcgcc cagttgtgga ggcggagaac atgaagccat
      961 ggcgcctgat tacccacaac tggagccaca aactggcgtc gttggcggcg gctctgctga
     1021 tctgggtcgt cacggtcaac gcgcagaacc ccattgagag cctcgaagtc gatgtgcccg
     1081 tcgaggtggc gtgtggacct gactacgcgg tgagagtgga acagggcatc accactgtgc
     1141 atgcggtcct caggggcgag cgggcactgc ttcagccatt tcgcgacggt cgccggaagc
     1201 tcgtcgccac cgtccgcctg cccgagcctc tcgaggaggg caggggccag ggcgaggcga
     1261 tgctcgcacc cttgccgggg agggtccggg tcgtgtcgct gacgccggcg actctggcct
     1321 accgcctgat ccgcctggac aggaagatcg ttagggttca gaccgaactt cgcggagccc
     1381 ccatcgaagg ggttgatttc ggcggcctgg tctgccaacc tccggaggtc gaagtggtgg
     1441 gccccaagtc ggtggtcgac gccgtggatt ccgtccggac catcgtgcgc cgcatcgacc
     1501 tggtgaacca ggacacggtc gaatccaagg ttcggcccat cgacgcccag ggaaggaccc
     1561 tcgacgatcc cgccctgacc gtgatgcgcc ccacggtggt ggtctcgctt ccgcccgtcg
     1621 agccaaaggc cgtcaaggtg tccgtgacca ccaccggcca ccttcccgac ggataccaga
     1681 tcacgggcat tcgggtcagc cccg
//
LOCUS       DAOSPL010001185         1703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_70903_length_1703_cov_2.301578, whole genome shotgun sequence.
ACCESSION   DAOSPL010001185 DAOSPL010000000
VERSION     DAOSPL010001185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70903_length_1703_cov_2.301578"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /gene="cimA"
                     /locus_tag="PLQ54_18015"
     CDS             complement(<1..1082)
                     /gene="cimA"
                     /locus_tag="PLQ54_18015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOS95217.1"
                     /translation="MGQSVSIYDTTLRDGCQGEGIALSLQDKLRIAQRLDDLGIHYIE
                     GGWPNPTNPKDVAFFRHVGALNLKHAKVSAFGSTRRGGNRPETDPTLQTLLGAETTVV
                     AIFGKSWDLHAEEILRVSLDENLEMIRSSVAYLAERGREVVYDAEHFFDGYKHNPEFS
                     LRVVEAAAVAGAESIVLCDTNGGSLPFEVEQIVGQVVTYLDAPVGVHLHDDTGCGVAN
                     ALAGVRGGATQVQGTINGYGERTGNCNLSTIIPNLSLKMGVDTIPAERLERLTTVSHH
                     VAELVNISPDPQQPYVGASAFAHKAGLHVSAIARRSDAYEHVNPDAVGNGTRFVVSEL
                     AGKSTLELKAREIGLELDGRELTEVVG"
     gene            complement(1470..1553)
                     /locus_tag="PLQ54_18020"
     tRNA            complement(1470..1553)
                     /locus_tag="PLQ54_18020"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1517..1519),aa:Leu,seq:cag)
BASE COUNT          257 a          575 c          581 g          290 t
ORIGIN      
        1 ccgaccacct cggtgagctc cctgccgtcg agctcgaggc cgatctccct ggccttcagc
       61 tcgagggtgg acttccccgc cagctcggac accacgaagc gggtgccgtt gcccacggcg
      121 tcggggttga cgtgctcgta ggcgtcggag cgtcgggcga tggcactgac gtgcaggccc
      181 gccttgtggg cgaaggccga cgcccccacg tagggctgct gggggtcggg gctgatgttc
      241 accagctcgg ccacgtggtg cgacaccgtg gtgaggcgct cgaggcgctc ggcggggatg
      301 gtgtccacgc ccatcttgag gctgaggttc gggatgatgg tcgacaggtt gcagttgccc
      361 gtgcgctcgc cgtagccgtt gatggtgccc tgcacctggg tcgcgccgcc gcgcacgccg
      421 gcgagggcgt tggccacacc gcagccggtg tcgtcgtgca ggtgcacgcc caccggggcg
      481 tcgaggtacg tgacgacctg gcccacgatc tgctcgacct cgaacggcag cgagccgccg
      541 ttggtgtcgc agagcacgat gctctcggcg ccggccaccg ccgcggcctc gaccacccgg
      601 aggctgaact cggggttgtg cttgtagccg tcgaagaagt gctcggcatc gtacaccacc
      661 tcgcgcccgc gttcggccag ataggcgacg gacgaccgga tcatctcgag gttctcgtcg
      721 agactgacgc gcaggatctc ctcagcatgc agatcccagc tcttgccaaa gatggccaca
      781 acggtcgtct cggccccgag cagcgtctgg agggtaggat ctgtctcggg gcgattgccg
      841 ccgcgacgcg tactgccaaa ggcgctcacc ttggcgtgct tgaggttcag ggcgccgaca
      901 tggcggaaga aggcgacgtc cttggggttc gtggggttcg gccacccgcc ctcgatgtag
      961 tgaatgccca gatcgtcgag gcgctgggcg atgcgcagct tgtcctggag cgatagggca
     1021 atgccctcgc cctgacagcc gtcgcgcaac gtcgtgtcgt agatgctcac gctctggccc
     1081 acggcaacac ctccacccgt gagggtcctg tggcccggaa acggaaaagt ccgtctccgt
     1141 agccggagac ggactggtcg gtccgcggta ccactccgct tccgatgcgc aatgcaccgg
     1201 ctctctaccg gccacgggct ttggccccac gggcacccgc cgatggcctg ccccgctaac
     1261 ggtgggcttc cggcagcgtc tactcgccca caacaggggc tttcgggctg caactcgcgg
     1321 gcgatcttcg gctgggcacc gctcccgggc tcgcaccatc cccggttcgc tgaagcagaa
     1381 ctcccagcgt actgttcccg gtcgtcgtct ttgtgggaac ggactggcta tgcaaggaac
     1441 aggcggctgc accgcgctag agaggccggt gccgaggagg ggagtcgaac ccctacgagg
     1501 gaaccctcac tggaccctga acccagcgcg tctgccagtt ccgccacctc ggcacgctcg
     1561 acagcgaaat gcatgttagg cacttcggtg ccccgtgtca agggccgggg gcggcgcgac
     1621 tgccgcggcg tttgacaccc ctgtgaggca ccggtatact tcacgcttcg agacggttcc
     1681 ggaccctgct ggcgcccggc gac
//
LOCUS       DAOSPL010001186         1702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_70943_length_1702_cov_2.952034, whole genome shotgun sequence.
ACCESSION   DAOSPL010001186 DAOSPL010000000
VERSION     DAOSPL010001186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70943_length_1702_cov_2.952034"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..576
                     /locus_tag="PLQ54_18025"
     CDS             19..576
                     /locus_tag="PLQ54_18025"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95218.1"
                     /translation="MAVLQDGQAPLVLRSGVFSPHRPVTLSFWWALPYDLTIEGAYTL
                     FSLTGRGYIALFCRGQGEWCALQRPAGVFQVYYFEDIRNINGIYDFDLLGHTDLRAGV
                     WHHTAVVFRRASTIQVYTDGDLSCEYTVMGGVFREVHELATLTLGGPLYLDELAVLDR
                     AIDAPAVAEYVQGMRQLRGYLRPAE"
     gene            778..1524
                     /locus_tag="PLQ54_18030"
     CDS             778..1524
                     /locus_tag="PLQ54_18030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95219.1"
                     /translation="MRRARWVGALAGTAVVVVLGLVAGCGAGDSLGVVGTSDPGASTR
                     LTGGSIPGGTGVQLEAYSLPKSGTDGAGSRSASVVASPAAWCWAQLKLASGRSLWAVT
                     DGAGVAQFVGVPLGSFSLTVWPPFGRSDLSPLTVTAARTDPAADVLVLGVMGPNGSPD
                     DGAIRLEANDTIVSPGESSDVLANVASGSVPAAGSSLAWVLETRTGAELTLDVTNVRH
                     AVIRSGSTRGTALVAGHFGPASSSCTFSLR"
BASE COUNT          237 a          527 c          643 g          295 t
ORIGIN      
        1 taagaccaga cgcccggtat ggcggtcttg caggacgggc aggcgccgtt ggtgctccga
       61 agcggcgtct tctcgcccca ccgtccggtg actctgagct tctggtgggc gttgccttat
      121 gacctgacaa tcgaaggggc ttacacacta ttcagcctga cgggccgcgg ctacattgca
      181 ctcttctgcc ggggccaagg cgagtggtgt gccctgcaac gcccggccgg cgtgttccag
      241 gtgtattact tcgaggacat ccgcaacatc aacgggatct acgacttcga tctgctcggt
      301 cacaccgatc ttcgcgcggg cgtctggcac cacacggcag tggtgttccg gcgggcatcg
      361 acgatccagg tctacacgga cggggatctg agttgcgagt acacagtgat gggcggcgtc
      421 ttccgggagg ttcatgagct ggccacgttg acactggggg ggccgctgta cctcgacgag
      481 ttggccgtac tcgaccgggc cattgacgct ccggcggtgg cggagtatgt gcaagggatg
      541 cggcagctcc gcgggtacct gcgcccagcg gaataggaga ggcctgcccc ggccggcggg
      601 ccgaaccggt ttttttgccg aatctcgctc gggtgggcca cagacagcgt acgagtacgg
      661 aacacagcca atacgagcgg ccatcggtgc ggcgggctgt gccccgcgat gaggcgccga
      721 tgcggggcgg gatggcgccg gagacggcgc ctgcggtacc ggggggcgga ggcaggaatg
      781 agacgcgcgc ggtgggttgg ggctctcgca gggactgcgg tggtggtggt actggggctg
      841 gttgccggct gcggagcggg cgactcgctc ggagtggttg gaacatccga cccgggcgca
      901 tcgacgcgcc tgactggcgg cagcatcccg ggcgggacgg gcgttcagct tgaggcgtac
      961 tccctcccga agtcgggcac cgacggcgcg ggctctcgca gcgcgagcgt tgtggcgtcg
     1021 ccggcggctt ggtgctgggc ccagctcaag ctcgctagtg gccgctcgct gtgggcggtg
     1081 accgacggtg cgggtgtcgc gcagttcgtg ggggtacctc tcggatcgtt ctcgctcacc
     1141 gtctggcctc cgtttggcag atcggacctg tcgccgctga cggtgacggc cgcgcgaacg
     1201 gacccagcgg cggatgtgct ggtgctcggc gtgatgggcc ccaacgggtc gcccgacgac
     1261 ggcgccatac ggctcgaggc caacgataca atcgtctcgc cgggtgagtc gtcggatgtg
     1321 ctggccaacg tggccagcgg gagcgttcct gctgccgggt cgagcctggc gtgggtactc
     1381 gagaccagga ccggcgccga gctgacgttg gacgtcacca acgtgcggca cgcggtcatc
     1441 cgatccggtt cgacacgagg gaccgctctg gtggcgggtc acttcggccc cgcctcgtcg
     1501 agctgcacct tctcgctccg ttagccgacc ggcctgcccg ctgatgcagg gccgcatcgg
     1561 atgggcccgg tccgccccct cgggggcgga ccgggcctcg gttcgcttgg ggtcttgggg
     1621 tgagagaagc cgtgggcgtc cctccgcgtg cctcgcagcc agggcttccg ggctggcggg
     1681 cagaggggca ggacccataa gg
//
LOCUS       DAOSPL010001187         1702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_70949_length_1702_cov_2.758956, whole genome shotgun sequence.
ACCESSION   DAOSPL010001187 DAOSPL010000000
VERSION     DAOSPL010001187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70949_length_1702_cov_2.758956"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /gene="tig"
                     /locus_tag="PLQ54_18035"
     CDS             complement(<1..1008)
                     /gene="tig"
                     /locus_tag="PLQ54_18035"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOS95220.1"
                     /translation="MQVSVEQLQGSQVRLAIEAATDDVGSAFDKAYREISRTVPVPGF
                     RPGKAPRQWIAQVIGREVVGERARELLVEETLKQAIEGQSLDILGEPRLDLPEEVPAE
                     GAEFSYSATVVVKPTFELPQYRGIPVEREVKAATDELVDEEIESLRAKHARIVPVTDR
                     PAQEGDLVDIEYFVYPADQLAPVSPRRMPMVLCLGQNWFSPSLDDDVVGMSLGDAKVI
                     TRTSGGQMMNYDIRIRNIQQRILPEVNEEFAMRTTGFESVEELREDIRTRIQSQFEAL
                     TRESAINRILARLAKECRFDLPDILIRAAVRRRLIAWVSALRNDNPEMAHYLASSDAA
                     LE"
     gene            complement(1205..1286)
                     /locus_tag="PLQ54_18040"
     tRNA            complement(1205..1286)
                     /locus_tag="PLQ54_18040"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1250..1252),aa:Leu,seq:tag)
     gene            complement(1303..1375)
                     /locus_tag="PLQ54_18045"
     tRNA            complement(1303..1375)
                     /locus_tag="PLQ54_18045"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1340..1342),aa:Lys,seq:ctt)
     gene            complement(1418..1490)
                     /locus_tag="PLQ54_18050"
     tRNA            complement(1418..1490)
                     /locus_tag="PLQ54_18050"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1455..1457),aa:Arg,seq:tcg)
     gene            complement(1512..1585)
                     /locus_tag="PLQ54_18055"
     tRNA            complement(1512..1585)
                     /locus_tag="PLQ54_18055"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1549..1551),aa:His,seq:gtg)
     gene            complement(1598..1670)
                     /locus_tag="PLQ54_18060"
     tRNA            complement(1598..1670)
                     /locus_tag="PLQ54_18060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1635..1637),aa:Arg,seq:tct)
BASE COUNT          309 a          548 c          549 g          296 t
ORIGIN      
        1 ctcgagggcc gcgtcgctgg aggcaaggta gtgagccatc tcggggttgt cgttgcgcag
       61 cgcggatacc caggcgatca gacgccggcg cactgcggcg cggatgagga tatctggcag
      121 gtcgaaacgg cactccttgg cgagacgggc caggatccgg ttgatcgcgg attcgcgagt
      181 gagcgcctcg aactggctct gaatgcgggt gcggatgtcc tcacggagct cctcgaccga
      241 ctcgaaacca gtggtcctca tggcaaactc ttcgttcacc tcggggagga tccgctgctg
      301 tatgtttcga atgcgtatat cataattcat catctgcccg ccgctggtgc gcgtgatgac
      361 cttggcgtcg cccagcgaca tccccacgac gtcgtcatcc agcgacgggg agaaccagtt
      421 ctggcccagg cagagcacca tcggcatgcg ccgaggactg accggggcca gctggtcggc
      481 cgggtacacg aagtactcga tgtcgaccaa gtcgccttcc tgcgccggtc ggtcggtgac
      541 gggcacaatg cgggcatgct tggcccggag ggactcgatc tcctcgtcga cgagttcgtc
      601 ggtggcggct ttgacctcgc gctccaccgg aatcccacgg tactggggca gttcgaaagt
      661 gggcttgacg accacggtgg cagagtagga gaactcggcc ccctcggcgg ggacttcctc
      721 gggcagatcg aggcggggct cgccgagaat gtccaggctc tgcccctcga tggcctgttt
      781 cagggtctcc tcgacgagga gctcgcgggc gcgttcgccg acgacctcgc ggccaatgac
      841 ctgcgcgatc cactggcgtg gcgccttgcc cggtcggaag ccgggaacgg ggaccgttcg
      901 ggagatctcc cgataggctt tgtcgaaggc ggaacccacg tcgtcagtgg ccgcttcgat
      961 cgccaggcgg acctggctgc cctgcagctg ctcgacagag acctgcatcg gcttgtacac
     1021 cctttcctgg tccggggcgt tacctccccc gggcgcggcc acgcgggtcg cgacagccgc
     1081 ccggcatcct tggccccggc ggcgaaaaaa ggggaggcct aacgtcgact tgccggactc
     1141 tgcagcttgg gctgcgaggc aaggccgatg ccagacctcc gacagacagg gggcggctat
     1201 gcggtgggcg aggagagact cgaactctca cgcctcgcgg cacatgatcc taagtcatgc
     1261 atgtctgcca gttccatcac tcgcccgtct cccgaaatca ggtgggccgt ggaggattcg
     1321 aacctccgac cttgggatta agagtcccct gctctaccag ctaagctaac ggccccagac
     1381 tggttgtacc tacccgcgaa acacacgagt caatcggtgc ggctgggaag attcgaactc
     1441 ccgacccgca gatccgaagt ctgctgctct atccactgag ctacagccgc acgggcacac
     1501 ttaagaaccg gggtgggtgg agggattcga accctcggcc tttggagcca caatccaacg
     1561 ctctaaccaa ctgagctaca cccaccatgc accgcggccg gcctggcccg actcgaacgg
     1621 gcgacccact gcttagaagg cagttgctct atcccctgag ctacaggccg cagacccgga
     1681 gcgggagacg ggagtcgaac cc
//
LOCUS       DAOSPL010001188         1699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_71127_length_1699_cov_4.143552, whole genome shotgun sequence.
ACCESSION   DAOSPL010001188 DAOSPL010000000
VERSION     DAOSPL010001188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71127_length_1699_cov_4.143552"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PLQ54_18065"
     CDS             complement(<1..772)
                     /locus_tag="PLQ54_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOS95221.1"
                     /translation="MSTCVTITNIRAILTQPAGSRLIVVRIDTSEPGLYGLGCATFTQ
                     RPLPVKVAVEEYLKPLLVGREVDRIEDLWQLSWVNSYWRNGPVLNNAISGVDMALWDI
                     KGKRAGMPVYDLLGGRCREAVALYRHADGSCPEEVEENVRRWMAEGYHYIRVQMGGYG
                     GRAGSMHTPEGAQPGAYFDDLAYARSVPRLLEHIRGTIGFEVELLHDVHERLRPIDAL
                     QLAKAVEPYGLFFLEDALAPEDVEYFETIRSQCATPLAM"
     gene            complement(816..>1699)
                     /locus_tag="PLQ54_18070"
     CDS             complement(816..>1699)
                     /locus_tag="PLQ54_18070"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOS95222.1"
                     /translation="SAPMRVLVTGGAGSVGRAVVPELLRRGHTVTVAGRTPGRTVEGA
                     RYEVLDCTQFEAVRRLVLEHESVIHLAAIPSPRRHDSREIFEANCLSTYYVFDACVEA
                     GISKIALASSINALGQLFGVRPLPVRYFPIDEDHPQLCTDPYSFSKKILEETAEYFWQ
                     RNGISSVSLRITMVVHPGDHMWRRLHDVPNRDPMEQYMVRNYWTWVDARDSARAFVDG
                     IEAPYQGAHPLFINDSVNNMGMPSRQLAAMAYPEVTDFREPLDGTESLVSVRRAKEIL
                     GWEPQVHFPQESPPAPE"
BASE COUNT          301 a          568 c          546 g          284 t
ORIGIN      
        1 ccatggccag cggggtggcg cactgcgacc ggatcgtctc gaagtactcg acatcctccg
       61 gcgccagggc atcctcgagg aagaacagcc catagggctc cacggccttg gccagttgca
      121 gggcgtcgat gggccgcagc cgctcgtgga cgtcgtgcaa cagctcgacc tcgaagccga
      181 tagtcccgcg gatgtgctcc agcaaccggg gcaccgaccg ggcgtacgcg aggtcatcga
      241 agtaggcgcc cggctgagcc ccctcggggg tgtgcatcga tcccgcccgg ccgccatacc
      301 cgcccatctg gacgcggatg tagtggtacc cctcggccat ccaccggcgg acgttctcct
      361 cgacttcctc agggcacgaa ccgtcggcgt ggcggtagag ggccacggcc tcccggcacc
      421 tcccgcccag caaatcgtag accggcatgc cggcgcgctt gcccttgatg tcccagagcg
      481 ccatgtcgac gccggagatg gcgttgttga ggacggggcc gttccgccag tacgagttga
      541 cccacgagag ctgccagagg tcctcgatgc ggtcgacctc gcggccaacc aacagtggct
      601 tgagatactc ctcgaccgcc accttgaccg gcaatggccg ctgggtgaat gtcgcgcaac
      661 cgaggccata gaggcccggt tcgctggtat cgatcctgac cacgatcagc cgcgaaccgg
      721 ccggttgtgt aaggatggcg cggatattgg taatggtgac gcatgtggac atggtagccc
      781 cttgtcatat gggcagatga gcataccgca gcagactact ctggtgccgg cggtgactcc
      841 tgggggaagt gaacctgcgg ctcccagccc aggatctcct tcgctcgccg cacgctgacc
      901 agcgactcgg tgccgtcgag gggctcccgg aagtcggtca cctcagggta ggccatggcg
      961 gccagctggc gactcggcat gcccatgttg ttgacggaat cattgatgaa tagtgggtgc
     1021 gctccctggt agggggcctc gatcccatcc acgaacgccc gggcgctgtc ccgggcatcg
     1081 acccaggtcc agtagttccg aaccatatac tgttccatcg ggtcacggtt cggcacatca
     1141 tggagccgcc gccacatgtg gtcccccggg tgaaccacca tggtgattcg cagcgacacg
     1201 ctcgagatcc cattccgctg ccagaagtac tcagccgtct cctccagtat cttcttcgag
     1261 aagctgtacg gatcggtgca gagctgcggg tggtcttcat cgatggggaa gtaacgcacc
     1321 ggcaggggcc ggacgccgaa gagctgcccg agggcgttga tggagctggc cagggcgatc
     1381 ttcgagatcc cggcctcgac gcacgcgtca aacacatagt aagtcgacaa gcaattggct
     1441 tcgaagatct cgcgtgagtc gtgccggcgt ggcgacggga tcgccgccag gtggatcacg
     1501 ctctcgtgct cgagcaccag ccggcgcacg gcctcgaact gggtgcagtc gagcacctcg
     1561 taccgtgcgc cctcgacagt ccggccgggc gtacggcccg ccaccgtcac cgtgtggcca
     1621 cggcgcaaaa gctcgggcac cacagcccgg cccaccgacc cggctccgcc tgtcaccagg
     1681 accctcattg gcgcagacc
//
LOCUS       DAOSPL010001189         1699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_71163_length_1699_cov_2.681265, whole genome shotgun sequence.
ACCESSION   DAOSPL010001189 DAOSPL010000000
VERSION     DAOSPL010001189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71163_length_1699_cov_2.681265"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="PLQ54_18075"
     CDS             <1..1316
                     /locus_tag="PLQ54_18075"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HOS95223.1"
                     /translation="SYVLARPATGAAADDPAAPAVGPRMDVVFLIDTTGSMADEIEVV
                     KEKMRDMVAQIASGDPAPRVRFGLVLYRDRGDEYVTKVIELTDDIDAMVAHIAEVQAA
                     GGGDEPESLNEALHVALHDMNWDPAEEVDKTVFLIADAPPHMDYEGDYQYAGEVEYAV
                     HHNIIIHSIGCSGLSEEGNSIFRQIAQGSEGTFNLLTYKREYVTADGTTETVLEAGGR
                     VFGIMGEPGAAADADWRVGAERLEEAGAARAIAAAPVPPATAAPGGAMGGGMMGGMGG
                     MGGTAMGAATENNLDSILAGTLRKQAERRGVRYADTGTLEIVARYTGTRSGIHEALRQ
                     VIRTEDEWKALWERHTQPFEPRPELPQVDFGKSMLIVVFSGEKATGGHSVRVETVRET
                     KDGLDVTFRVTKPDEGMMTTQAFTQPYDIVVVDNTEGELRFLEAE"
     gene            1460..>1699
                     /locus_tag="PLQ54_18080"
     CDS             1460..>1699
                     /locus_tag="PLQ54_18080"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS95224.1"
                     /translation="MAELRCGVVGLGRGRLFVERLNAQPDCRVTAVCDTNDRALAGLS
                     GLECYTDFEEMLDSERLDLVAIVGPGPVHASQSARA"
BASE COUNT          304 a          526 c          618 g          251 t
ORIGIN      
        1 ctagctacgt gctggcgcgg cctgccaccg gggccgccgc agacgatccg gcggcacctg
       61 cggtgggacc gcggatggac gtagtgttcc ttatcgatac cacgggcagc atggccgatg
      121 agattgaagt ggtgaaggag aagatgcgcg acatggtcgc gcagatcgcg tcgggcgacc
      181 cggccccgcg cgtgcgcttc ggcctcgtgc tctaccgcga ccgcggggac gagtacgtga
      241 ccaaagtcat cgaactgacc gacgacatcg acgccatggt ggcccacatt gccgaggtcc
      301 aggcggcggg cggaggcgat gagcccgaga gcctcaacga ggcgctccat gtggccctgc
      361 acgacatgaa ctgggacccg gccgaggagg tcgacaagac ggtcttcctc atcgctgacg
      421 ccccgccgca tatggactac gagggcgact atcagtacgc gggcgaggtc gagtacgcgg
      481 ttcatcacaa catcatcatc cactccatcg gctgcagcgg gctcagcgag gaggggaact
      541 cgatcttcag gcagatcgcc cagggcagcg aagggacgtt caacctgttg acctataagc
      601 gcgagtacgt cacggccgac ggcacgacgg agacagtgct cgaggcgggg gggcgggtgt
      661 tcggcatcat gggcgagccg ggtgcggcag cggatgccga ctggcgcgtc ggcgccgagc
      721 gcctggagga ggctggggcg gcgcgagcca tcgcggccgc gccggtccct ccggccaccg
      781 cggccccagg cggagcgatg ggcggcggta tgatgggcgg aatgggtgga atgggtggga
      841 cggcgatggg tgccgcaacc gagaacaacc tcgactcgat cctcgccggc accctccgca
      901 agcaggctga gagacgcggt gtgcgctacg ccgacacggg taccctcgag atcgtggcgc
      961 gctataccgg aacccgaagc ggcatccatg aggccctgcg gcaggtaatc cgcaccgagg
     1021 acgaatggaa ggcgctctgg gaacggcaca cccagccgtt cgagccgcgc ccggagctgc
     1081 cccaggtgga cttcgggaag tccatgctca tcgtcgtgtt ctcgggagag aaagccaccg
     1141 gggggcactc ggtccgggtc gagacggtcc gtgagacgaa ggacggtctg gatgtgacgt
     1201 tccgggtcac caagccggac gaggggatga tgaccacgca ggcgttcacc cagccctatg
     1261 acatcgttgt cgtcgacaac acggagggcg agttgcgctt cctggaggcc gagtagcggc
     1321 actgggcctc gaggcagact ccatcctgct gcgctcgccg agcccacata gcggtttgtc
     1381 gtgaaggcgc acggactgcc gcccccgaac gcgtaggccg gtgatcatac ccgacacctt
     1441 cgacggcggg gggacctcta tggcggaact gcgctgcgga gttgtggggc tcggtcgcgg
     1501 gcggctcttc gtcgagcgac ttaacgcaca gcccgactgc cgtgtcacgg cggtgtgcga
     1561 cacgaacgac cgggctctgg ccgggctcag cggactggag tgctacacgg atttcgagga
     1621 gatgctcgac tcggagcggc tggacctggt ggccatagtc ggcccgggtc ccgtgcatgc
     1681 atcgcagtcg gctcgtgcc
//
LOCUS       DAOSPL010001190         1699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_71168_length_1699_cov_2.523723, whole genome shotgun sequence.
ACCESSION   DAOSPL010001190 DAOSPL010000000
VERSION     DAOSPL010001190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71168_length_1699_cov_2.523723"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLQ54_18085"
     CDS             complement(<1..262)
                     /locus_tag="PLQ54_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95225.1"
                     /translation="MAAAAIFGCAVIVPSVCLAQIGAEFSLDFSSRGLLASVRQASLL
                     ATLLVCGWMAQKHGKAPFLSAGMVMIAVGMACLALAPDHATVL"
     assembly_gap    365..374
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<375..>1699)
                     /locus_tag="PLQ54_18090"
     CDS             complement(<375..>1699)
                     /locus_tag="PLQ54_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95226.1"
                     /translation="GALFGLCVALAVPRARPELFAAVGALAFSLGGSMTYGQTIGLVQ
                     NAPGSPTHGWGMLGLAVKGGVWIGLGSLLLGALLLGPPVRCRSLLFVLGVAFLWFVVG
                     RALVNGPHSPPQRLPLIYFSDRHDPKPRPECWGGLWLAYLWLLGWAARFRTPESVRLS
                     LYGLAAGAVGFPLGESLQAWGHWAHPFGAFGDRWVDWWKVMEVAFGAVAGAGIGLGAT
                     RLRGQVAHSPPETAVRVWESGLLVLWCAAYTLADMQVLPLGGTLYLPFLGIALPLLLC
                     ASSEARSRLPSAHMVGCILVLTLSAADLVAKAHEEALPGLMAVAALAVTCIGLGALIA
                     GRQALLGRLSGGWLIIFIGGTQTLLVAAKSCMEGPTWAEGARGFWHGSSVTVVAGFCA
                     ATAAVAALLGASGVVSVRVAPEGESPRPGVRVEGLRARRTNVSGRTRRRR"
BASE COUNT          334 a          612 c          569 g          174 t
ORIGIN      
        1 ccaggacggt ggcatggtcg ggtgccaagg ccaggcatgc cattccaacc gcgatcatca
       61 ccatgcccgc actgaggaaa ggcgccttgc cgtgcttctg ggccatccag ccgcagacga
      121 gcagcgttgc cagcaacgat gcttgcctca cgctggcgag caggccccgg ctcgagaagt
      181 cgagcgagaa ctcggcgccg atctgcgcca gacagaccga gggcacgatg accgcgcaac
      241 caaagatggc ggccgctgcc atggtcccgg catacagacg acgatggacg gggggcaggg
      301 cgggctgttg gtctggcggg cagggaacgc tcagagaacc acaactctca cgcgcgccgg
      361 tccgnnnnnn nnnncgcctc cggcgcgtcc ggccactgac gttcgttctg cgggcccgaa
      421 gcccttcaac gcgcactccc gggcgcggac tctctccttc gggtgctaca cggaccgaca
      481 ccacgcccga ggcccccagc agcgccgcca ctgcagccgt ggccgcgcag aagcccgcca
      541 ctacggtgac ggacgagccg tgccagaacc ctcgagcgcc ctcggcccac gtggggccct
      601 ccatgcagct cttggcagcc accaggagcg tctgcgtgcc gccgatgaag atgatgagcc
      661 atccgcccga cagcctgccg aggagcgcct ggcgacccgc gatgagcgcg cccaggccga
      721 tgcaggtgac ggccagagcc gcgacggcca tcagacccgg cagtgcctcc tcgtgcgcct
      781 tcgccaccag gtccgcggcc gagagagtca gcacgagtat gcagccgacc atgtgggcac
      841 tcggaagccg actccgcgcc tcagaagacg cgcagagcag aaggggcagg gcgatgccga
      901 ggaacggcag gtagagcgta ccacccagcg gcaacacctg catgtccgcc agcgtgtagg
      961 cggcgcacca tagcaccagg agaccggact cccagacacg gactgcggtc tcgggcggcg
     1021 aatgagccac ctgcccgcgc aggcgagtgg cgcccaggcc gatgcccgcc ccggcgacgg
     1081 cgccgaaggc gacttccatc actttccacc aatcgaccca gcggtcaccg aacgcgccaa
     1141 acggatgggc ccaatgcccc caagcctgga gcgactctcc gagcgggaac cccacggcgc
     1201 ccgcggccaa gccatagagc gagagtcgga ccgactcggg cgtgcggaag cgggccgccc
     1261 agccgaggag ccacaggtag gccagccaca agccccccca gcactcgggc cgaggcttgg
     1321 ggtcgtggcg atcggagaag tagatcagcg gtaaccgctg gggcgggcta tgtggcccgt
     1381 tgaccagggc gcggcccacc acgaaccaca ggaaggcaac gcccagcacg aacagcagcg
     1441 agcggcaccg cacgggagga ccaagcagca gcgccccgag cagcagggag ccgaggccga
     1501 tccagacgcc acctttcacg gcgaggccga gcatccccca cccgtgcgtg ggcgaaccag
     1561 gagcgttctg gaccagcccg atggtctggc cataggtcat cgatccgccg agcgagaacg
     1621 ccaaggcgcc gaccgcggcg aagagctccg gacgggcccg gggcacagcc agtgccacgc
     1681 agagcccgaa cagagcgcc
//
LOCUS       DAOSPL010001191         1696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_71335_length_1696_cov_2.582572, whole genome shotgun sequence.
ACCESSION   DAOSPL010001191 DAOSPL010000000
VERSION     DAOSPL010001191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71335_length_1696_cov_2.582572"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLQ54_18095"
     CDS             complement(<1..315)
                     /locus_tag="PLQ54_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95227.1"
                     /translation="MLTQFSTAQGFCPGGGSVTRFAQFAPRFRLAVGVLWAFPAFGAP
                     AEPLHPSFVDSWLFGETIAYGQQTVALGSARPDEVRIEPAYRGDPRYGRIRFGHDPGV
                     CVA"
     gene            complement(339..1493)
                     /locus_tag="PLQ54_18100"
     CDS             complement(339..1493)
                     /locus_tag="PLQ54_18100"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOS95228.1"
                     /translation="MIRLRYCVMIATVVNALGLYWFGTVLLRQPAPEDEATTRVRLVS
                     LGPMVQEPVRRLAVPPAPAGVGRGTVGPVYRPGTFGEGGAAPWTNAGPVSERQAIAPG
                     ASTAAPGESGSPAPGASQRPLAGLGGSEDQPVPSPHEQADVESPLPPAPATAGAGMAD
                     QRPIGDLSPDPIKPSSVQAGQKGPGESAPGRGRPPSGDGSGPSAGPGPGGTATPDASP
                     TLKPGSPTGTGGGGGASGAAGGAAGGGTQSATGQGGTGGGKGRGSASSGTGTGGGGGR
                     GPAAGSPGGGAGSAPAPAAPKETDPVKISGPPPPRTREMERLEIHGAISVRVRIGPDG
                     RVQEATVIKGSGYAQYDRTAASWVQSNWRFRPGTRGGTAVTKTIVVSVRY"
BASE COUNT          231 a          619 c          609 g          237 t
ORIGIN      
        1 ggcgacacag acgcccggat cgtgtccgaa gcggatccgg ccataccttg ggtcgccgcg
       61 atatgccggc tctatcctga cctcgtcggg tcgagcactg ccgagtgcaa ccgtctgctg
      121 gccgtaggcg atcgtttcgc caaagagcca cgagtccacg aagctcggat ggagcggctc
      181 cgcaggcgct ccgaacgccg ggaaggccca gaggacgccg acggccaggc gaaaacgcgg
      241 ggcaaactga gcgaagcggg tcaccgagcc acccccgggg cagaagccct gtgcggtgct
      301 gaactgcgtg agcacgaagg gctcgcgctg ccacagtatc agtagcggac agataccaca
      361 atggtctttg tgaccgctgt gccgccgcgg gtgccgggcc ggaaccgcca gttgctctgc
      421 acccatgagg ctgccgttcg gtcatactga gcatagcctg agcccttgat caccgtggct
      481 tcctggacgc ggccgtcagg accgattcgg acgcgcacgc tgatggcgcc gtgaatctcg
      541 agtcgctcca tctcgcgtgt acgcggaggc ggggggccac tgatcttcac ggggtcggtc
      601 tccttgggcg cggcgggagc gggagcgctc ccggcgccgc cgcccgggct cccagcggcg
      661 gggccccggc cgcctcctcc gccggtgccg gtccctgacg atgcggaccc gcgtcccttg
      721 ccgcccccgg tgccgccctg gccggttgcg ctctgcgtcc caccgccggc cgcgccaccg
      781 gcggcgccgg acgcgccgcc gccgccgccg gtgccggtgg gcgaacccgg cttcagggtc
      841 gggctggcat cgggcgtggc agtccctccg gggcccggcc ccgctgaggg gcctgaaccg
      901 tcgcccgatg gcggacgccc gcggccaggg gccgactcgc cgggaccctt ctgcccggcc
      961 tgaacactgg agggcttgat gggatcgggc gataggtctc cgatgggccg ctggtcagcc
     1021 atccccgcgc cggccgtggc gggcgccggc ggcagcggcg actcgacatc ggcctgctcg
     1081 tgcgggctgg ggacgggctg gtcctccgag ccgccgaggc ccgcgagagg tcgctggctg
     1141 gctcccgggg ccggcgaccc ggattcaccg ggtgccgcgg tgctggcgcc cggcgcgatg
     1201 gcctggcgct cggacactgg gccggcattg gtccacgggg cggcgccccc ttcgccgaag
     1261 gtgccgggac ggtacacagg tccaacggtg ccacgtccca cacccgccgg agccgggggt
     1321 acggccagac gccgtaccgg ctcctgcacc atcgggccca gcgatacgag ccgcacgcgc
     1381 gtggtggcct cgtcctccgg cgccggctgg cgcaacagca ccgtgccgaa ccagtagaga
     1441 cccagcgcgt tgacgaccgt cgcgatcatc acgcagtaac ggagtctgat catgcttcgt
     1501 tagggctctt cccgtcagca ggggcttccg ccccgtccga tgacttggac atccggtgac
     1561 tccgcaagtt ccaccagccc ccgggtccac acaggtaacg ccagcatggc ccggcgggtt
     1621 ccggccgtcg ggggccgaga cggccgaact cggtgagtct cccggccgtt gtgaccacac
     1681 gagcgccact gcggcc
//
LOCUS       DAOSPL010001192         1695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_71379_length_1695_cov_3.424390, whole genome shotgun sequence.
ACCESSION   DAOSPL010001192 DAOSPL010000000
VERSION     DAOSPL010001192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71379_length_1695_cov_3.424390"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PLQ54_18105"
     CDS             complement(<1..276)
                     /locus_tag="PLQ54_18105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95229.1"
                     /translation="MTVLVVGRKAKRFLWAQFAGGPGSSGGFGSTDSQGPPTYRRPAR
                     QSDDELVLRSQRGDAEAFAELARRHEGRIYGIVYQYVKNQDDALDITR"
     gene            complement(340..>1695)
                     /locus_tag="PLQ54_18110"
     CDS             complement(340..>1695)
                     /locus_tag="PLQ54_18110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95230.1"
                     /translation="GGRSLLGGRVGSDDLYIRRAGESMTYSVLSIRLGMVEWVRDLIF
                     GDIMCSTRIALPHVYDPLDTSTYPEFVRRFAYATGKHTEGNEAGDTGLLLAAEALRKS
                     GDAERCRYAQRLIEDSFRHMVDRMQAPSGATIMWTDELADAGMGAAGSGGSYPFGVYD
                     SNQIGEWMRPLAYGIIYYRDVPGREPYARGLSAACRRAADYLVAHSTQDSDGLPSVIR
                     HLALAEREDGSVQQTIYRQEGRQCDVYLGRALSGLSYYAYAMQLLGERVPDDWWPVLD
                     NTVEWSARKMRDDGWFDWQCEDIVEGGCHTFLGNIYIGEGLFGCYLASKVAGREEAAA
                     RAAEATLRAYHYVTDHCYIKGTKYEYPLEFWVGPYVYWLFTEYQDAIGPDARLQDWLT
                     TLDRRWRVERGWRDFLDRAPAGGCGRTESNGMLTVSILGYLGLRQMDEIGRPLHWPLA
                     P"
BASE COUNT          288 a          601 c          529 g          277 t
ORIGIN      
        1 gcgcgtaatg tcgagcgcat cgtcctgatt cttcacgtac tggtagacga tgccgtaaat
       61 ccggccctcg tgccgcctcg ccagctcggc aaatgcctcc gcgtctcccc gctggcttcg
      121 caggaccagc tcgtcatcgg actggcgcgc cggccgtcga taggtcggcg gtccctgcga
      181 gtcagttgag ccaaaacccc cggacgagcc aggcccgccg gcgaactgcg cccacagaaa
      241 ccgcttcgcc ttccgtccta cgacgagtac cgtcacatcg ggttccctaa gcgcgcaatg
      301 attccagccc aatggggccg gtgccctgtt cccagttgcc taaggcgcca gcggccagtg
      361 aaggggccgg ccgatctcgt ccatctggcg caggccgagg taccccagaa tcgacacagt
      421 aagcatacca ttactctctg tgcggccaca gccacccgcc ggggcgcgat cgaggaagtc
      481 ccgccagccg cgctccacgc gccagcggcg gtccagagtt gtcagccagt cctgcaggcg
      541 tgcgtccggg ccgatggcgt cctggtactc ggtgaacagc cagtacacgt agggcccgac
      601 ccagaactcg agcgggtact cgtacttggt ccccttgatg tagcagtggt cggtcacata
      661 gtggtaggcc ctcaacgtgg cctcggccgc ccgggccgct gcttcctcac ggccggccac
      721 cttgctggcc aggtagcagc cgaacagccc ctcgccgatg tagatgttcc caagaaacgt
      781 gtgacagcca ccctcaacga tgtcctcgca ctgccagtcg aaccagccgt cgtccctcat
      841 cttccgcgcc gaccactcga cggtgttgtc cagcaccggc caccagtcat cgggcacgcg
      901 ctcgcccaga agctgcatcg cataggcgta atacgagagc ccagagagtg cccggccgag
      961 atacacatcg cactgccggc cctcctgccg gtagatggtc tgctgcaccg aaccgtcttc
     1021 cctctccgcc agcgcgagat ggcggatgac gcttggcagg ccgtcggagt cctgcgtcga
     1081 gtgggccacc aggtagtcgg cggcccgccg gcaggcggcg ctcaaccctc gggcataggg
     1141 ctcgcggccg ggcacgtcgc ggtagtagat gatgccgtag gccagcgggc gcatccactc
     1201 gccgatctgg ttcgagtcgt acacgccgaa cggatagctc ccgcccgagc cggccgcgcc
     1261 catccccgcg tcggccagct catcggtcca catgatggtc gccccggagg gcgcttgcat
     1321 gcggtcgacc atgtgacgga agctatcctc gatcagtcgc tgggcatagc gacaccgttc
     1381 ggcatcgccc gacttccgga gtgcctcggc ggccaggagc aagccggtat cgccggcctc
     1441 gttcccctcg gtgtgcttgc cggtggcgta tgcgaaccgc cgcacgaact cgggataggt
     1501 ggacgtgtcg agcgggtcgt acacatgcgg cagcgcgatg cgcgtcgagc acatgatgtc
     1561 gccgaagatg aggtcgcgga cccactcgac catgccgagg cggatcgaga gcaccgagta
     1621 ggtcatgctc tctccggcac ggcggatgta gaggtcatcg ctccccactc ggcccccgag
     1681 aagcgagcgg ccccc
//
LOCUS       DAOSPL010001193         1691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_71608_length_1691_cov_2.929707, whole genome shotgun sequence.
ACCESSION   DAOSPL010001193 DAOSPL010000000
VERSION     DAOSPL010001193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71608_length_1691_cov_2.929707"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PLQ54_18115"
     CDS             complement(<1..613)
                     /locus_tag="PLQ54_18115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95231.1"
                     /translation="MMEYAAVDESLRRGISPEFIGHLTSGVLLPVLERVRHDDTLSLE
                     IRNGYVDIYYRGGRVLGIHDNGASGRFRAEFDTNYCNIDPAYRPTLPKEPPRSIASTD
                     DAAEWVEVLASYKQVMDVFFSANPKLEREFEQLAERANAQHTIGEESDYLVLDIEYTQ
                     SSLACPGWASDCRFDMVGFRWPIAGKTRGCRLVTPVIMEMKVGD"
     gene            complement(712..1266)
                     /locus_tag="PLQ54_18120"
     CDS             complement(712..1266)
                     /locus_tag="PLQ54_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95232.1"
                     /translation="MIDSAFSALPAETQIALARLSDAVDGVRPAAVRAWPIDPLALVE
                     EATASSLLREVSDWASAGQACLYYVECRSADVDLTEIAAAFAAAKARKRRARAYPRLN
                     SLGTCLYVGSSRSMGKRLREHLGYGAAGTYALQLAHWATPLSLELDFVCAKYAEDTRP
                     EVMQALEDTLWQVRRPMFGRRGAR"
     gene            complement(1306..>1691)
                     /locus_tag="PLQ54_18125"
     CDS             complement(1306..>1691)
                     /locus_tag="PLQ54_18125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95233.1"
                     /translation="ADVVFLDPDNGLEVGCGPCGDKGPKYAFYDDLSGISAGQTLIVY
                     QHAIRTGTFEEQLEARMDDLARSLRRPLENLLAMRWRRVSPRAFIFALADPRRAEGLE
                     RLRAMLAGPWGGSFDLVRPGLLEQA"
BASE COUNT          274 a          561 c          586 g          270 t
ORIGIN      
        1 cgtcgcccac cttcatctcc atgatgaccg gggtgaccag gcggcacccg cgcgtcttgc
       61 ctgcgatggg ccagcgaaag ccgaccatgt cgaagcggca gtccgacgcc catcccgggc
      121 aggccagcga tgactgggtg tactcgatgt cgaggacgag gtagtcgctt tcctccccga
      181 tcgtgtgctg cgcgttggcg cgttcggcca gctgctcgaa ctcgcgctcg agcttcggat
      241 tggccgagaa gaagacgtcc atgacctgct tgtaggaggc gagtacctcg acccactcgg
      301 cggcgtcgtc cgtggaggcg atgctcctcg ggggctcctt gggaagggtg gggcggtagg
      361 ccgggtcgat gttgcagtag ttggtgtcga actcggcgcg gaagcgtccc gaggcgccat
      421 tgtcgtggat gccgagcacc cgaccgccgc ggtagtagat atcgacgtag ccgttcctga
      481 tctccaggct gagcgtgtcg tcgtggcgga cccgctcgag cacgggaagc agcacccccg
      541 aggtgagatg ccctatgaac tccggtgaga tgcctcgccg caagctctcg tcgaccgccg
      601 cgtactccat cattctcatt ctccttcagc agcagccggc gacggctgtg tggataggca
      661 tcccgcgtgt ggcgggcggc caggacgtgc acggaggcga gggaacgctc ttcacctggc
      721 gccccggcga ccgaacatcg gccgcctgac ctgccacaac gtgtcctcga gagcctgcat
      781 cacctcagga cgagtgtcct ccgcgtactt cgcgcagacg aagtccagct ccagtgagag
      841 cggcgtcgcc cagtgtgcca gctgaagcgc gtaggttccc gcggcgccgt agccgaggtg
      901 ctccctgagt cgcttcccca tcgaccggga gctgccgacg tagaggcaag tccccaggga
      961 gttcaggcgc gggtaggcgc gcgcgcgtcg cttgcgggcc tttgccgcag cgaaggcggc
     1021 tgcgatctcg gtgagatcga cgtccgcgga tcggcactcg acgtagtaca ggcatgcctg
     1081 acccgcgctg gcccagtccg agacctccct gaggaggctg gaagcggtcg cctcctccac
     1141 cagcgcgagg gggtcgatcg gccaggccct gacggcggcg ggacggacgc cgtcgaccgc
     1201 atccgagagt ctcgccagag cgatctgggt ctctgccggc agtgctgaga aggcggaatc
     1261 aatcatgtga gtcacagcgc gaccggcgcg aagccccgtc ccatctcagg cctgctccag
     1321 gagtcctggg cgcacgaggt cgaagcttcc gccccagggc cccgcaagca tcgcccggag
     1381 cctctcgagg ccctcggcac ggcgcgggtc agccagggcg aagatgaagg cgcgcgggct
     1441 gacgcgccgc cagcgcatgg cgagaaggtt ctccagcggc cgacgaagcg agcgcgccag
     1501 gtcgtccatg cgggcttcga gctgctcctc gaaagtcccg gtacgaatcg cgtgctggta
     1561 gacgatcagg gtctggccag ccgagatccc cgagaggtcg tcgtagaagg cgtacttcgg
     1621 ccccttgtcg ccgcaggggc cgcagcccac ctcgaggccg ttgtcggggt cgaggaacac
     1681 cacgtccgcg c
//
LOCUS       DAOSPL010001194         1689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_71754_length_1689_cov_2.392289, whole genome shotgun sequence.
ACCESSION   DAOSPL010001194 DAOSPL010000000
VERSION     DAOSPL010001194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71754_length_1689_cov_2.392289"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PLQ54_18130"
     CDS             <1..359
                     /locus_tag="PLQ54_18130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95234.1"
                     /translation="VLMPPDASDNVPADKDSVWEVIELKHIGAGSVAQILGGSVFSLN
                     SDLMLLGRQGTPGRGGRSWNNGGWGNGGWGDNGWGNRGWGNGGWGNNRWGNSGWGANG
                     WTQNGWGQGGYGGRAW"
     gene            457..675
                     /locus_tag="PLQ54_18135"
     CDS             457..675
                     /locus_tag="PLQ54_18135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95235.1"
                     /translation="MMLVKLTEMDDGTVKAETARAVLGFFEDMTVSDVAAFLVKRAED
                     VGETIRFVDKLDEPTEHIDFQRLMKRKF"
     gene            complement(688..>1689)
                     /locus_tag="PLQ54_18140"
     CDS             complement(688..>1689)
                     /locus_tag="PLQ54_18140"
                     /inference="COORDINATES: protein motif:HMM:NF020091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOS95236.1"
                     /translation="AVPWGIHTHNDAGCAVANAALAVHLGASQVQGTINGYGERCGNA
                     NLCSVIPNLVLKQGIPALSREALTHLSSVSRFISEMVMEAPDERQPYVGNSAFAHKGG
                     AHIDAVRKKTVSFEHVAPELVGNERRILVSEQSGTATIIEKLHTTYPHLDKRDPAVGR
                     LLERLKHLEYEGYEFEAAEGSFHLLAEQILRPRDPFFVLENYRVLDGRHGAGDTYTEA
                     TIRVRVGDQVRHTAAEGTGPVDALARALREALVPFYPELEHIRLTDYKVRVLNGEAGA
                     SARVRVLIQTTDGHESWGTVGVSEDILAASWEALVDSLTFGLRRARSREMASGGGPA"
BASE COUNT          296 a          538 c          581 g          274 t
ORIGIN      
        1 ttgtcctcat gcctccggac gccagcgaca acgtgccggc ggataaggac tccgtgtggg
       61 aggtcatcga gctcaagcac atcggcgccg gaagcgtcgc ccagatcctc ggaggcagcg
      121 tcttcagcct gaactctgac ctgatgctgc tcggccgtca ggggacgcct ggccgcggcg
      181 gccgctcgtg gaacaacgga gggtggggca acggcggttg gggagacaac ggctggggca
      241 accgcggctg gggaaatggc ggctggggca acaaccgctg ggggaacagc ggctggggcg
      301 ccaacggctg gacccagaac ggatgggggc aaggcggata cggcggacgc gcctggtagg
      361 tgcgatggcg gccaaggagc tgcgcctcgg gcggcgaaca acacccccag ctggttcggc
      421 actgcggtgc tggccgtggg acgggagtgg tcgcccatga tgctggtgaa gctcaccgag
      481 atggacgacg gcacggtcaa agccgagacg gcaagagccg tgctcgggtt cttcgaagac
      541 atgacggtgt cggatgtcgc cgcctttctc gtcaaacgcg cggaggatgt gggcgagacg
      601 atccgtttcg tcgacaagtt ggatgagccc acagagcaca tcgacttcca acgcctcatg
      661 aagcgcaagt tctagcaacc acgggcgtca ggcgggtcct ccgcctgacg ccatctcgcg
      721 tgagcgcgcc cggcgcagac cgaaggtgag gctgtcgacc aacgcctccc agctcgccgc
      781 caggatgtcc tccgataccc caacggttcc ccagctctcg tgtccgtcag tggtctggat
      841 cagcacccgg actcgggcgg aggccccggc ctcgccgttg agcacgcgga ccttgtagtc
      901 ggtcaggcgg atgtgctcca gttcggggta gaagggcacg agcgcctccc gcaatgcccg
      961 tgccagggca tccaccggac ccgtgccctc tgcggccgtg tggcggacct ggtcaccgac
     1021 ccggacgcga attgtcgcct ccgtgtaagt gtctccggcc ccatgccggc catcgaggac
     1081 gcgatagttc tccagcacga aaaacgggtc gcggggccgc agaatctgct cggccaggag
     1141 atgaaaagag ccctcggcgg cctcgaactc atacccctca tactccaggt gcttgaggcg
     1201 ctccagcaac cgacccaccg cggggtcgcg cttgtcgagg tggggatagg tcgtgtgaag
     1261 cttctcgatg atcgttgccg ttccggactg ctccgagacc aggatgcgac gctcattgcc
     1321 cacgagctcg ggcgcgacgt gctcgaacga cacggtcttc ttgcgcacag cgtcgatgtg
     1381 agccccgccc ttatgggcga aggcggagtt gccgacatac ggctggcgct cgtccggggc
     1441 ctccatgacc atctcggaga tgaaacgcga cacactgctc aggtgcgtga gcgcctctcg
     1501 cgagagcgca gggatgccct gcttcaggac caggttcggg atgacggaac agaggttggc
     1561 gttgccgcag cgctccccgt agccgttgat ggtgccctgc acttggctgg cccccagatg
     1621 gaccgccagc gccgcgttgg ccacggcaca ccctgcgtca ttgtgtgtgt gaatgcccca
     1681 tggtaccgc
//
LOCUS       DAOSPL010001195         1688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_71815_length_1688_cov_2.932639, whole genome shotgun sequence.
ACCESSION   DAOSPL010001195 DAOSPL010000000
VERSION     DAOSPL010001195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71815_length_1688_cov_2.932639"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="PLQ54_18145"
     CDS             complement(<1..1233)
                     /locus_tag="PLQ54_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95237.1"
                     /translation="MTRAMLAPPVPAVLDRGSAHRIIPHPVRVRDFPVSPIHAPAVLV
                     LAMVILGALPARPEESLVPPTYPKGRRLVSADARDWQIDIVRALFIPDAGARRIVRYI
                     QDLGGENPKLRAFRVSRNAWQAPREIHGFYASWASRNGFHPLIEVRLGTPPGVRYGDP
                     IDRYPRPTPYHPRLWPPAIPNTSPPFSFARPPKWEREDYAWLTAFYRPGDDGGVLVIV
                     WVRGVHIWVWRPGHCPVGPILSEWLGLRWAPTETAPPEKVALPDRPPPGMPVPGDTDY
                     LVRAELTKWELDAVAHDLQARAKQGPVRTPLDAFFTVIPQVVQPVSQAWVTTYRGPAA
                     RRASLLKPWVDWLSRSNWPLLHQGTYDAVPVRIWSQGGPNGGAVATFDVDDTTTVLAL
                     RGGVNLLAMMPVMATMRQA"
     gene            1437..1670
                     /locus_tag="PLQ54_18150"
     CDS             1437..1670
                     /locus_tag="PLQ54_18150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95238.1"
                     /translation="MLDGLARADLPPPWLTTEGSICIADRAVWTLEVTRDGEPLGEPA
                     EAMLEAPNLVGTSDPKRRARKCTLRGRRTSKVV"
BASE COUNT          266 a          573 c          600 g          249 t
ORIGIN      
        1 ggcctgcctc atcgtggcca tgaccggcat catggccagc agattcacgc cgccacgcag
       61 cgccagcacc gtggtggtgt cgtcgacgtc aaacgtggcg acggcgccgc cgttggggcc
      121 gccctggctc catattcgca ccggcacggc atcgtaggtc ccctggtgca gcagcggcca
      181 gttggaccgg ctcagccagt cgacccatgg cttcagcagg gacgcgcggc gcgccgccgg
      241 tccacggtag gtcgtcaccc acgcctggga gaccggctgc actacctggg ggatcaccgt
      301 gaagaaagcg tccagcgggg tcctgacggg cccctgcttg gcccgcgcct gcaggtcgtg
      361 agcgacggca tccagctccc acttcgtcag ctcggcgcgg acgagatagt cggtgtcacc
      421 gggtaccggc atgccgggtg gcggccgatc gggcagggcg accttctccg gcggggctgt
      481 ttcagtgggc gcccatcgga gccccagcca ctcgctgagg atcgggccca cgggacagtg
      541 gcccggacgc cacacccaga tgtgcacccc acggacccac acgatgacga gcacgccgcc
      601 gtcatcgccg gggcggtaga aggccgtgag ccaggcgtag tcctcgcgtt cccacttcgg
      661 cgggcgcgcg aaactgaacg gaggcgaggt gttggggatg gccgggggcc agagccgcgg
      721 gtgatacggt gtcggccgcg ggtaccggtc gattgggtca ccgtaccgaa cgccgggcgg
      781 cgtgccgagc cttacctcga tcagcggatg gaaaccattg cgggaggccc agctcgcata
      841 gaagccgtgg atctctctcg gggcctgcca tgcgtttcgg ctcacgcgga acgcgcgcag
      901 cttggggttc tcacccccga ggtcctggat atagcgcacg atcctccggg cgcccgcgtc
      961 ggggatgaac agggctcgaa caatgtcgat ctgccagtcg cgagcgtcgg cactgacgag
     1021 gcgcctgccc ttggggtacg tcggcggcac caagctctcc tcgggccgcg cagggagcgc
     1081 gccaagaatg accatggcga ggaccagcac cgcgggagcg tggatggggc tgaccgggaa
     1141 gtcgcggacg cgaaccggat gggggatgat cctgtgtgcg gaaccgcgat ccagcaccgc
     1201 cgggaccgga ggcgcaagca tggcccgagt catgctcgcc cgccgggcac ccgtgacggc
     1261 atgcctcgct gcggtccgtg aagatacgtg ctccatggtc acaccctcgc gtactcttcc
     1321 ggacttacta gaacccgtcc tgccgcgtct gttgcggtag cggggcccaa tcagcgtcca
     1381 cgtgccgcgt ctcgcgtccg gaagaacgat gcgcttcgac ccggggggcg ggcggcgtgc
     1441 tcgatggctt ggcccgcgcc gacctgccgc cgccgtggct caccaccgag ggatcgatct
     1501 gcatcgccga ccgagccgta tggaccctcg aagtcactcg cgatggcgag ccgctcggcg
     1561 agcccgcgga ggccatgctc gaagccccca atctcgtcgg gaccagcgac ccgaaacggc
     1621 gtgcgcggaa gtgcaccctc cggggtcgcc ggacgtctaa ggttgtgtga aggatggcct
     1681 ccggggct
//
LOCUS       DAOSPL010001196         1688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_71827_length_1688_cov_2.582976, whole genome shotgun sequence.
ACCESSION   DAOSPL010001196 DAOSPL010000000
VERSION     DAOSPL010001196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71827_length_1688_cov_2.582976"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PLQ54_18155"
     CDS             complement(<1..467)
                     /locus_tag="PLQ54_18155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOS95239.1"
                     /translation="MRRLNGIVWVFLLVEFIDELTYGANEAAWPLIRRDLGLSYTQIG
                     LLLSIPNLFGSLVEPALGVLGDTRWRRTIVLTGGVVFAVTLAGVAGAWCFPVMLAALL
                     LHYPASGGFVNLAQAELMDVAPDRRDQNMARWTAAGSLGVVVGPLILGGACLIG"
     gene            complement(503..1636)
                     /locus_tag="PLQ54_18160"
     CDS             complement(503..1636)
                     /locus_tag="PLQ54_18160"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS95240.1"
                     /translation="MALKVGIVGFRGIAVPHADSHQADPLADLVAICDVVPERARAGA
                     EKYGCKAYNSLKDMLDGEPDLDIVDVCTGGYENGSWHYEPAMEAMEAGKHVLVEKPIS
                     NDINEAREMVAKAREKDVYLGCNLNHYFTPPAEKAREYMDSGAIGELVYCLHKMGFNG
                     GEPEGYQAPKSRKIAGWPWFHLKAFMSHPLSVMRYFCGEVTHVQAFVNRPGFRRRVGE
                     EMLSTTSIHLRYENDCVGYLLSQRGDASFGLGGWWSLEVAGTQGTFCIENCIEKITYW
                     PAPASGSAGKPENMDPGAAGSGIVTNSGITDFGQTFPRRIHAFLEDVTNGVHKEDLRA
                     SGRDALAALEITFAVMESSQEGGATVRPHPLPPIHGDPTVLLD"
BASE COUNT          288 a          574 c          539 g          287 t
ORIGIN      
        1 ccgatcaggc acgcgccgcc gaggatcaga gggccgacga cgactccgag cgagccggct
       61 gccgtccacc gagccatgtt ctggtcgcgc cggtcggggg ccacgtccat cagctcggcc
      121 tgggccaggt tcacgaaccc gcccgatgcg gggtagtgca gcaggagggc ggcgagcatc
      181 acggggaaac accatgcccc ggccacgccg gccagggtga ccgcgaagac cacgccacca
      241 gtcagcacga tggtccgccg ccagcgggtg tcgccgagga cgcccagggc gggctcgacg
      301 aggctcccga acaggttggg gatgctcagg aggaggccga tctgggtgta gctcagcccc
      361 agatcgcggc ggatgagggg ccaggccgcc tcgttagcac cgtaggtgag ctcgtcgatg
      421 aactccacaa gcaggaacac ccagacgatg ccgttcagac gtctcatcgt gcgacctggc
      481 ccgctaacgg cacatccgca gatcagtcga gcagcaccgt cggatcgccg tggatcggcg
      541 ggagcgggtg cggacgcacc gtggctccgc cttcttggga ggactccatg accgcgaagg
      601 tgatctccag cgccgccaac gcatcccggc ccgaggcccg cagatcctcc ttgtggaccc
      661 cgttggtgac gtcctcaagg aatgcgtgga tgcggcgcgg gaaggtctgc ccgaagtccg
      721 tgatgcccga gttcgtcacg atgccgctgc ccgccgcgcc ggggtccatg ttctcgggct
      781 tgcctgcgct gcccgaggct ggtgcgggcc agtaggtgat cttctcgatg cagttctcga
      841 tgcagaacgt gccctgcgtc ccggcgacct cgaggctcca ccagccgccc aagccgaacg
      901 acgcatcgcc ccgctggctc aacaagtagc cgacgcagtc gttctcgtac cgcaggtgaa
      961 tgctggtggt cgagagcatc tcctcgccga ctctccggcg gaacccggga cggttcacga
     1021 acgcctgcac gtgggtgacc tcgccgcaga agtagcgcat cacgctcagc ggatgcgaca
     1081 tgaaggcctt caggtggaac cagggccagc cggctatctt ccgggacttg ggtgcctggt
     1141 agccctccgg ttcgccgcca ttgaagccca tcttgtgcag gcagtagacc agctcaccaa
     1201 tggcccccga gtccatgtac tctcttgcct tctcggccgg cggagtgaag tagtgattga
     1261 ggttgcagcc gaggtacacg tccttctctc gagccttggc aaccatctcg cgagcctcgt
     1321 tgatgtcgtt cgagatgggc ttctctacca gaacatgctt gccggcttcc atggcttcca
     1381 tggccggctc gtagtgccag ctcccgttct catacccgcc cgtgcacacg tcaacgatgt
     1441 ccagatcggg ctcgccgtcg agcatgtcct tgaggctgtt gtatgccttg cacccgtact
     1501 tctcggcccc ggcgcgcgcg cgctcgggga cgacgtcaca aatggccacc agatcggcca
     1561 gcggatcggc ctgatgcgaa tccgcgtgag gcaccgcgat gccccggaac ccgacgatac
     1621 ccacctttag agccatggtc gctcctcctg tacccgttca cacggaatgc ggcgagtgtg
     1681 ccacactc
//
LOCUS       DAOSPL010001197         1687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_71866_length_1687_cov_3.257966, whole genome shotgun sequence.
ACCESSION   DAOSPL010001197 DAOSPL010000000
VERSION     DAOSPL010001197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71866_length_1687_cov_3.257966"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PLQ54_18165"
     CDS             <1..796
                     /locus_tag="PLQ54_18165"
                     /inference="COORDINATES: protein motif:HMM:NF028184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH36 C-terminal domain-containing protein"
                     /protein_id="HOS95241.1"
                     /translation="TCYRQDFNMDPAPFWEAADAPDRVGMAEIRHIEGLYRYWDALRE
                     RIPGLLIDNCSSGGRRIDLETLSRSIPLWQSDFQCFLGFDPIGLQGQTHGLSMWVPLH
                     SGACDRPDDYALRSAIGPGLVFCTPPNFEGTPEGYLTPWDAFSAAWLRKAADEQKTVR
                     PYYAGDFYPLLSYTLADDAWAAWQCDRPDLGEGCVMALRRPKSPFASMAPKLHGLDAE
                     ATYELRDPDTGTAWQATGKHLMESGLSLSIDAAPGSRLVLYRRVEG"
     gene            complement(907..>1687)
                     /locus_tag="PLQ54_18170"
     CDS             complement(907..>1687)
                     /locus_tag="PLQ54_18170"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOS95242.1"
                     /translation="EPVALQSVRDAGFDVLDLANNHSTDCGRPGLVDTMRWLRRNHLR
                     WCGAGATMAEAESPTVVAAGNLRVAFVSFSDWLPEGLFPLEDRPTIALASPEAVARAA
                     KRAASDADVVVVGFHWGIEFGSTPTPRQRELAQAAVGAGAALVVGHHPHVLQGLEWMP
                     GPNDRPALVAYSLGNFVFDQYRNGSANGALLECTLTRNGAEEARLVPIRITHCRPALA
                     DGNEARQILSHLNRLSAGRGTAVWPDGVVARQQRDRRGTGR"
BASE COUNT          239 a          559 c          613 g          276 t
ORIGIN      
        1 cacctgctac cggcaggact tcaacatgga cccggcgccg ttctgggagg ccgcggacgc
       61 ccccgatcgg gtcggcatgg cggagatccg gcacatcgag gggctctacc gctattggga
      121 tgccctacgg gagcggattc ccgggctgct gatcgacaac tgctcgagcg gcggccggcg
      181 aatcgacctc gagaccctct cgcgaagcat ccctctctgg cagagcgact tccagtgctt
      241 cctggggttc gacccgatcg gtctgcaggg gcagacgcac gggctctcga tgtgggtgcc
      301 tctgcactcg ggcgcttgcg accggccgga cgactacgcc ctccggagcg ccatcggtcc
      361 ggggctggtg ttctgcacgc cgccgaactt cgagggcacg cccgagggct atctgacgcc
      421 gtgggatgcc ttcagtgcgg cgtggctccg gaaggcggcg gacgagcaga agacggtgcg
      481 gccgtactat gcgggagact tctacccgct gctgtcgtac acgctggccg atgatgcgtg
      541 ggcggcatgg cagtgcgacc ggcccgacct gggtgagggg tgcgtcatgg ctctgcgccg
      601 gccgaagagc cccttcgcct cgatggcgcc gaagctgcat gggctcgacg ctgaggcgac
      661 gtatgagctg cgcgacccgg acacgggcac ggcctggcag gccacaggca aacacctgat
      721 ggagagcggc ctgagcctgt cgatcgacgc cgccccgggg tcacggctgg ttctgtatcg
      781 gcgggtggaa gggtaggctc gggacagggc ccctccacca ggccgggccg tgcggtgtat
      841 gggttttccg cgcaccgccc gggcctcagt gactgcccat gggggttaag cgggacgtgc
      901 gccggctcac cggcccgtgc cgcggcggtc cctctgctgt ctcgccacga ccccatcggg
      961 ccagactgcg gttccgcggc ccgccgagag acggttcagg tggctgagga tctgtcgcgc
     1021 ctcgttcccg tctgccagtg cggggcggca gtgagtgatg cggatcggca cgagccgggc
     1081 ttcctcggct ccattgcgcg tgagcgtgca ctcgagcaga gcgccattgg cgctgccgtt
     1141 gcggtattgg tcgaacacga agttgcccag ggagtaggcc accagagcgg gcctgtcgtt
     1201 cggtccgggc atccattcca gtccttggag cacgtgcggg tgatgaccca ctaccagcgc
     1261 cgcgcccgcc ccgactgctg cttgggccag ctcgcgctgt cgcggtgttg gcgtcgagcc
     1321 gaactcgatg ccccagtgga agccgacgac caccacatcg gcatcgctcg cggctcgttt
     1381 ggctgccctg gccactgctt cgggcgaggc cagggcgatg gtgggccggt cctcgagtgg
     1441 gaagagtccc tcggggagcc agtccgagaa gctcacgaat gcgacgcgca ggttgcctgc
     1501 ggcgaccacg gtggggctct cggcctcggc catggttgcc ccggcgccgc accagcgcag
     1561 gtggttccgt ctgagccagc gcatcgtgtc gacgaggccc ggccggccgc agtccgtcga
     1621 gtggttgttg gcgaggtcga gcacgtcgaa gcccgcatcg cgaacagact gcagcgccac
     1681 gggctcg
//
LOCUS       DAOSPL010001198         1685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_71996_length_1685_cov_2.957669, whole genome shotgun sequence.
ACCESSION   DAOSPL010001198 DAOSPL010000000
VERSION     DAOSPL010001198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71996_length_1685_cov_2.957669"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="PLQ54_18175"
     CDS             complement(<1..169)
                     /locus_tag="PLQ54_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778317.1"
                     /note="catalyzes the conversion of glutamine residues to
                     glutamate on methyl-accepting chemotaxis receptors;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOS95243.1"
                     /translation="MGLSIPVGMAEVKASKTADDSLSALGLGSCIGVCVYDPRARVAG
                     LAHVMLPDSAVI"
     gene            complement(179..877)
                     /locus_tag="PLQ54_18180"
     CDS             complement(179..877)
                     /locus_tag="PLQ54_18180"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HOS95244.1"
                     /translation="MRDAGRPGATGSLSGDAGTGTRGILFMAFEAEGITELQLDALRE
                     VGNIGAGHAATALADLLGEEIDITVPTAGLRRTEEIAASPEAAEAMVVGVLFIVEGDA
                     GGVIVYVFSEADARFLSDRLMGLEPGTTQQIDEMGCSMLGEVGNILSGSFVTALAEFT
                     HLDFGLSPPNVAVDTLATILSEITVTAEALGELSLELDTHFVQQSGQLEGRFYYLMDR
                     DSLGAILGALGMAG"
     gene            complement(1266..1649)
                     /locus_tag="PLQ54_18185"
     CDS             complement(1266..1649)
                     /locus_tag="PLQ54_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95245.1"
                     /translation="MLDSEASRIAGAGPDAVLRELIRLSRDQHTALCAGDLERVREFM
                     EARAELLRVWGSEYRPRPEDASSVTDELRCALASADADLARALEERLGATAEELRQVR
                     RARRAWRGYLEPRGTGAGRIVDRTG"
BASE COUNT          294 a          649 c          490 g          252 t
ORIGIN      
        1 ggatgaccgc gctatcgggc agcatcacgt gcgccagccc ggcgacacgc gcccgggggt
       61 catacacgca caccccgata caggacccca gacccagcgc cgacagcgag tcgtccgccg
      121 tcttgctcgc cttcacctcg gccatcccca ccggtatcga cagccccacg agctcctcct
      181 accccgccat gccgagcgcg cccagaatgg cgcccaggct gtcccgatcc atcagatagt
      241 agaatcggcc ctccaactgc ccgctctgct ggacaaagtg cgtatcgagt tccagcgaca
      301 gctcaccaag cgcctcggcc gtcaccgtga tctcactgag gatcgtcgcg agcgtgtcca
      361 cggccacgtt gggtggcgag aggccgaaat ccaagtgcgt gaactccgcc agtgccgtca
      421 cgaatgaccc cgacaggata ttcccgactt cgccaagcat cgagcatccc atctcgtcga
      481 tctgctgtgt cgtgcccggt tcgagcccca tgagccggtc agacaagaag cgcgcgtccg
      541 cctcggagaa cacatagacg atgacaccgc ccgcatcgcc ctcgacgatg aacaggactc
      601 cgaccaccat cgcctccgcg gcctccgggc tggccgcgat ttcctctgtc cggcggagcc
      661 ccgcagtggg cacggtgatg tcgatctcct caccgagcag gtccgccagc gcggttgcgg
      721 cgtggcccgc tccgatgttg cccacctcac gcagcgcgtc cagctgcagt tcggtgatgc
      781 cctcggcctc gaatgccatg aacaggatcc ccctcgttcc ggttcccgcg tcgccgctca
      841 gcgagccagt ggcccccggc cgcccagcgt ccctcattcg ggcagcctca tacccgcgtc
      901 tcgccgggag cgaggcactc aggctcagac ccgaacgtcc accctgaccg ggttcgatgc
      961 gctctgcgca ataccgcgct cagtacggcc cgttgccaca tggtactgat gcgtcaatga
     1021 tgcggaagcc cgaagccgag tgctgcccga ttcggagggg cttcggtagc aggtaaggct
     1081 ctcggcaatg atcgtgccgt cgtgtgcacg agcctcacgc cgcatccgcc ccagcgcgcc
     1141 gacggcctcc cccaagaccc ggagatgtgc ctgggaatcg gggacacgaa ccgagatccc
     1201 cactccacga agggtgacca cactcgcacc ccctgactcg gtccgctcag gctcttgagc
     1261 acggcctagc cagtgcggtc cacgatccgg cccgctcccg tgccacgcgg ttcgaggtag
     1321 cctcgccacg ctcgccgcgc gcgacggact tgccggagct cctcagccgt ggcgcccaac
     1381 cgctcctcaa gcgcgcgcgc caagtcggca tcagcagacg ccagcgcaca gcgcagctca
     1441 tcggttaccg aagaggcgtc ttcggggcgc ggccggtact cggaccccca tacacgcaac
     1501 aactcggccc gggcctccat gaactcccga acgcgctcaa gatcgccggc gcacagggcc
     1561 gtgtgttggt cgcgcgacag ccggatcagc tcgcgcaaga cggcgtccgg cccggcccca
     1621 gcaatccgag acgcctcgct gtcgagcacg gcccgacccc tccagaccac gtacgctgtt
     1681 caggc
//
LOCUS       DAOSPL010001199         1685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_72008_length_1685_cov_2.392025, whole genome shotgun sequence.
ACCESSION   DAOSPL010001199 DAOSPL010000000
VERSION     DAOSPL010001199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72008_length_1685_cov_2.392025"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..>1685
                     /locus_tag="PLQ54_18190"
     CDS             276..>1685
                     /locus_tag="PLQ54_18190"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HOS95246.1"
                     /translation="MAARQVHLVCNAHLDPVWLWEWEEGAAEAVSTFRTAADLCEEFD
                     GFIFNHNEVILYRWIEEYEPELFARIQRLVAEGRWHICGGWYLQPDCNMPSGESFVRQ
                     ILAGRRYFRSHFNAVPTVAVNFDSFGHCWGLPQILRKSGYEGYLCCRPGPGEIELPQD
                     TFIWVGADGSEVLATRSSVWYNSPLGGARNKVEAWLADHPDEPCGLVLWGVGDHGGGP
                     SRNDLRDLASLLAEHPEHGIVHSTPEGFHAGQRAARGDFPQWRRDINPWGPGCYTSMV
                     RIKQKHRRLECDLYTLEKMAASAWVQGLMDYPSDEISEALQDLLTCEFHDILPGSSAP
                     TVEEMALRLLDHGLEIASRLRARAFFALCSGQPAPADGEIAILAYNPHPFAVDTILEV
                     EFQLADQNWEDTFTNPLVHQNGVAVPCQCEKEQSNLTLDWRKRVAFRATLAPSQMNRF
                     DTKLEVLPKKPALPTIVDDA"
BASE COUNT          279 a          591 c          524 g          291 t
ORIGIN      
        1 cgcggccgat gctctcacga actgcctgcg cgtcactccc gagttcatga cgacccaccc
       61 tccgaacgca aggggcgccc gacgcgcccg atgatgccaa cccggtcacg ggcaggccct
      121 ggcacaggat gggcgctgct cccacgtacc ttcgggcggc gacccggttc cattcgcggt
      181 gccgcacgct cgcgcctccg gcaggttatt cggccggcct gtcccgaatg catacgccag
      241 cccgcccgtg cggtaccacg aaggaggcgc ttcctgtggc agcacgccaa gtgcatctgg
      301 tctgcaatgc tcacctcgac cccgtgtggc tgtgggaatg ggaagagggc gccgccgagg
      361 ccgtgtcgac cttccgcact gccgccgatc tgtgcgagga gttcgacggc ttcatcttca
      421 accacaacga ggtcatcctg taccggtgga tcgaggagta tgaacccgag ctgtttgccc
      481 gcatccagcg ccttgtggcc gagggccggt ggcacatctg cggcggctgg tatctccagc
      541 ccgactgcaa catgccctcg ggtgagtcgt tcgtccggca gattctggcc gggcggcgct
      601 acttccgcag tcacttcaac gcggtcccga cggttgccgt gaacttcgac tcctttggcc
      661 actgctgggg cctgccacag atcctgcgaa agagcggcta tgagggctat ctatgctgcc
      721 gccccgggcc gggtgagatc gagctgccgc aggacacgtt catctgggtg ggcgcggatg
      781 gctccgaggt gctcgccacc cggtcatcgg tctggtacaa ctcccctctg ggcggcgcgc
      841 gcaacaaggt cgaggcgtgg cttgccgacc acccggacga gccctgcggc ttggtcctct
      901 ggggtgtcgg cgaccacgga ggcggaccat cgcgcaacga cctgcgtgac ctggcgtcac
      961 tgctcgcgga gcaccctgag cacggcatcg tccattccac tcctgaggga ttccacgctg
     1021 gccagcgggc ggcccgtggc gacttccccc aatggcgccg cgacatcaac ccgtgggggc
     1081 cgggctgcta cacctcgatg gtccgcatca aacagaagca ccgccggctt gagtgcgacc
     1141 tgtacaccct ggagaagatg gccgcatcgg cctgggtaca gggcctgatg gactacccat
     1201 cggacgagat ctccgaggcg ctccaggatc tcctgacctg cgagttccac gacatcctcc
     1261 cgggatcgtc ggcacccacg gtggaggaga tggctctgcg ccttctggac cacggcctcg
     1321 agatcgcgtc ccgcctgcgg gcccgagcct tcttcgccct atgctccggc caacccgcac
     1381 ccgccgatgg ggagatcgcg atccttgcct acaaccccca cccgttcgca gtcgatacca
     1441 ttcttgaggt cgagttccag ctcgccgacc aaaactggga ggacaccttt acgaatcccc
     1501 tcgtgcatca gaacggtgtg gcggtgccgt gccagtgcga gaaggagcag tcgaacctca
     1561 ctctggactg gcgcaagcga gtggccttcc gcgccacctt ggcgccgtcc cagatgaacc
     1621 gcttcgacac caagctcgaa gtgctcccga agaagcccgc actgccgact attgtcgacg
     1681 acgcg
//
LOCUS       DAOSPL010001200         1684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_72036_length_1684_cov_3.890731, whole genome shotgun sequence.
ACCESSION   DAOSPL010001200 DAOSPL010000000
VERSION     DAOSPL010001200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72036_length_1684_cov_3.890731"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="PLQ54_18195"
     CDS             complement(<1..1236)
                     /locus_tag="PLQ54_18195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95247.1"
                     /translation="MRIASVLCLSACSCVTAAWSQAPRNMGVTLNGEAELAEPLAYDV
                     WHTIVASYRYPGAIDLLTNTYVVIARGGDQRSGFYIGYHLPRNELAIVKHGFWNEAEA
                     TGAPGEAGKIIENDQGFLDCEHTTVTRTADDITVSYRIRLKPGVLKGTCNVFQYVEDK
                     DVRYDGFTIVGSVTVGEDAGVHRTDMPDRWRNSLKPDGKPSEPLLLAEGGKARYALVV
                     PADAQRIEQKAAADLRANLQMICNAEFQIVSEAEAVADAGPFVSVGRTALLAGSRCKW
                     KDADLAAEGYAIEVAGDNVYLYGGTGRGLMNAAYSLLEEDLGCRWYSTTSVDTPRMDR
                     LEVRLVPRKYVPVLELRDPYILKMHDPTWSLRNKTNTPHARVPMSWGGSIRYHHMGHT
                     YARYFPTEQYFAEHPEYYAL"
     gene            complement(1233..1571)
                     /locus_tag="PLQ54_18200"
     CDS             complement(1233..1571)
                     /locus_tag="PLQ54_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95248.1"
                     /translation="MPEDGPNIRLACEFCHERGLILTNHSWGAASLLGRHAREYPNAT
                     LITGHLATDDEYCRVVKEHPNVYMGTCLPIHRPDLATTLRKLDGDNLLFGSVAPNVRP
                     AAPQPDRRSS"
BASE COUNT          251 a          541 c          585 g          307 t
ORIGIN      
        1 cagggcgtag tactcggggt gctcggcgaa gtactgctcc gtcgggaagt acctggcgta
       61 ggtgtggccc atgtggtggt accggatgct gccaccccac gacatcggga cccgtgcgtg
      121 cggcgtgttc gtcttgttcc gcagcgacca cgtggggtcg tgcatcttca ggatgtaggg
      181 gtcgcggagc tcgagcacgg gcacgtactt ccgtggtacc aggcgcacct cgagtcggtc
      241 catccgaggc gtgtcgaccg aggtggtcga gtaccatcgg cagcccaggt cctcctcgag
      301 cagcgagtag gccgcgttca tcaggccgcg gccggtgccg ccgtagaggt acacgttgtc
      361 ccccgcgacc tcgatggcat agccctcggc ggccaagtcc gcgtccttcc acttgcagcg
      421 ggacccggcc agcaaggccg ttcggcccac gctgacaaag gggccggcgt ccgccacggc
      481 ctctgcttcg ctgacgatct gaaactccgc attgcagatc atctgaaggt tggcgcggag
      541 gtcagccgcg gccttctgct cgatccgctg ggcgtccgcc ggaacgacga gcgcgtaccg
      601 ggccttgccc ccttcggcca gcagcagcgg ctccgagggc ttgccatcgg gcttcagcga
      661 gttccgccac ctgtcgggca tgtcggtgcg gtgcactccg gcatcctccc ccacggtcac
      721 ggagcccacg atggtgaatc catcgtaccg gacgtccttg tcctccacat actggaacac
      781 attgcacgtt cccttgagga caccgggctt gaggcggatg cggtaggaga ccgtgatgtc
      841 gtcagccgtc cgggtcaccg tggtgtgctc gcagtccagg aagccctggt cgttctcgat
      901 gatcttgccg gcctcgccgg gcgcgccggt ggcctccgcc tcgttccaga acccgtgctt
      961 gacgatggcc agctcgttcc gcggcaggtg gtagccgatg tagaacccgc ttcgctggtc
     1021 gccgcccctg gcgatgacca cgtaggtgtt ggtcagtagg tcgatggcac ccgggtagcg
     1081 gtacgaggcc acgatggtgt gccatacgtc gtatgccagc ggctccgcca attcggcctc
     1141 gccgttgagc gtgaccccca tgttgcgcgg ggcctgggac caggccgcgg tgacgcacga
     1201 gcaggccgag aggcacagga cagaggcgat tctcatgagg acctcctatc aggctgggga
     1261 gccgccggcc gcacgttcgg ggccacggag ccgaacagca gattgtcgcc atcgagcttc
     1321 cgcagcgttg tcgcgagatc gggccggtgg atcggcagac acgtgcccat gtagacgttc
     1381 ggatgctcct tcaccacgcg gcagtactcg tcatcggtgg ctagatggcc cgtgatgagc
     1441 gtggcgttcg ggtactctcg ggcatgccgg cccagcaggg atgcggcgcc ccaggagtgg
     1501 ttcgtcagga tcagccctcg ctcgtggcag aactcgcacg ccaggcggat gttggggccg
     1561 tcctcgggca ttggcggcaa cttagggttc cagtggccca tccgcctggt tgcggagggg
     1621 cgccatgggg gcattggcgg taacttaggg ctccagcggc ccatgggcct ggttgcggag
     1681 gggc
//
LOCUS       DAOSPL010001201         1677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_72433_length_1677_cov_3.866215, whole genome shotgun sequence.
ACCESSION   DAOSPL010001201 DAOSPL010000000
VERSION     DAOSPL010001201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72433_length_1677_cov_3.866215"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="PLQ54_18205"
     CDS             complement(<1..168)
                     /locus_tag="PLQ54_18205"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS95249.1"
                     /translation="MTDLAIVGAGPAGLSASIYAARAGLSVRLYEKALPGGQMNNTES
                     VENYLGFPGVLS"
     gene            complement(174..836)
                     /locus_tag="PLQ54_18210"
     CDS             complement(174..836)
                     /locus_tag="PLQ54_18210"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOS95250.1"
                     /translation="MAVVELHSHTCEYSGCAIDSTVDMLRAARTAGLDAIVITDHDHL
                     YPERELVRRVDEAQVDGLVALAAQEVSTWDRSGWRGDFLVFGAPQAKGRMTPEELIEW
                     AHAHGAVVVAAHPYRNGPRIGDQLYHLDVDGVEVINGSDPPEAVEPARAVAEERGLAR
                     TGGSDAHRAEQVGRCVTVFDGQVRSIADIVSGIRARRCSPRQGAHVVPGPVTRDLMRG
                     LR"
     gene            complement(843..>1677)
                     /locus_tag="PLQ54_18215"
     CDS             complement(843..>1677)
                     /locus_tag="PLQ54_18215"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS95251.1"
                     /translation="KPLARTVEEARAMGSAAAASDLACQVTFHCRFVPALMRAKQLIE
                     RGVLGRVFHFRAAYLHAGYTDPARPMSWRLDKEQSGGGALFDLGAHVVDVVRYLLGDV
                     RRVHGSLRTFIAERPAPDGSGPRPVEVDDLALMQVEMNDGSVGLVEASRLATGSNDQL
                     RIEIHGQKGALQFDLEEPGWLRVYRAEGPEEPYGGERGYTRIECMQRYPKPAVWPGPK
                     FVPGWLRFHAASVHAFLTHMVEHSPASPSFADGVACQEVLHAVQQSSACGRWVDVARE
                     S"
BASE COUNT          281 a          604 c          531 g          261 t
ORIGIN      
        1 cgagagcact cccggaaacc ccagatagtt ttcaaccgac tcggtgttgt tcatctggcc
       61 gccggggagc gccttctcat atagtcttac cgacaggcca gcccgcgcgg cgtaaatgga
      121 cgccgacagc cccgccgggc ccgcaccgac aatcgccagg tcggtcatgg cggtcacctc
      181 agtcctctca tcaggtcgcg agtcacggga ccgggcacca catgggcgcc ctggcgagga
      241 gaacaacggc gtgcccggat cccggaaacg atgtccgcga tggaccggac ctggccatcg
      301 aacaccgtga cacaccgtcc gacctgctcg gcgcgatggg catcgctccc ccccgttcgt
      361 gccaggccgc gctcctcggc gaccgcacgc gccggctcta cggcttcagg aggatccgag
      421 ccgttgatca cctccacgcc gtccacgtcg aggtgataga gctggtcacc aatgcgaggg
      481 ccgttgcgat aggggtgcgc cgccaccacg acggcgccgt gtgcgtgggc ccattcgatg
      541 agctcctcgg gcgtcatccg gcccttggcc tgcggcgccc cgaacacgag gaagtcgccg
      601 cgccagccgg atctatccca cgtcgagacc tcctgggccg ccagggcgac cagtccgtcc
      661 acctgggctt cgtccacgcg gcgaacgagt tctcgctccg ggtacagatg gtcgtggtcg
      721 gtgatgacga tggcatcgag cccggccgtg cgggccgctc gtagcatgtc caccgtggag
      781 tcgatggcgc accccgagta ctcgcaggtg tgactgtgga gctcaacaac tgccatcgct
      841 actcagctct ctcgggcgac gtcgacccac cgcccacagg cactcgactg ctgcacggcg
      901 tggagcacct cctggcacgc cacgccatcg gcgaacgagg ggctcgcggg gctatgctcg
      961 accatgtgag tgaggaacgc atgtacactg gcggcgtgaa accgcagcca gcccggcacg
     1021 aacttgggtc cgggccacac cgcgggcttc gggtagcgct gcatgcactc gatcctggtg
     1081 tagccgcgct cgccaccgta gggctcctcc ggtccctcgg cgcggtacac ccggagccac
     1141 cccggctcct ccaggtcgaa ctggagggcg cctttttgcc cgtggatctc gatccgaagc
     1201 tggtcgttgg atccggtcgc cagccgagag gcctcgacca gccccacgct gccgtcattc
     1261 atctcgacct gcatcagtgc caggtcatcc acctcaaccg ggcgggggcc ggacccgtcg
     1321 ggcgccgggc gctccgcgat gaacgtgcgc aggctgccat ggacccgccg gacgtcacct
     1381 agcagatatc ggaccacgtc cacgacatgg gccccgagat cgaacaaagc tcccccaccc
     1441 gactgctcct tgtcgagtcg ccagctcatc gggcgagccg ggtcggtgta gcccgcgtgc
     1501 aagtaagccg cacggaagtg gaatacgcgc ccgaggactc cgcgctcaat cagttgcttc
     1561 gcccgcatga gagccgggac gaagcgacag tgaaacgtca cctggcacgc gagatccgat
     1621 gctgccgcgg cgctccccat ggcgcgcgcc tcctccacag ttcgtgccag gggcttg
//
LOCUS       DAOSPL010001202         1677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_72445_length_1677_cov_3.163379, whole genome shotgun sequence.
ACCESSION   DAOSPL010001202 DAOSPL010000000
VERSION     DAOSPL010001202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72445_length_1677_cov_3.163379"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1554)
                     /locus_tag="PLQ54_18220"
     CDS             complement(<1..1554)
                     /locus_tag="PLQ54_18220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95252.1"
                     /translation="MRAERPIPVGGGLTITLQADLMGAPGAADSLAALDRWFELHGYP
                     EPLAMPRGDARAELAFSLQGYAKQHALWNPEWGCWYSDLIVGFHPNREPAHELLIGAR
                     ILAGGAEAEQAAALAAEVMGGDQAARDYAVEFRSSAGALADLAAQARGLIANQAADGT
                     WRFSGEHAKAGWTAEVIDYDYLGAEGASEVGLTAPSAATVLEYALLTGDPQAEEAGLR
                     ALRAMRRFRVPRAAQVWECPVHSPDILASAVAVNAYLLGYELTGDRAHLADAAYWART
                     GLPFVYVWSAPDQPAMQGASIPIFGATSYILSWLGVAVQWNGMAYAEALRRLARYDDS
                     FPWERVAENLLVSGMYQQATEGDRLAQWPDALNFIHGRPGLHGQTPPCFTPTTLVYAN
                     LAHRNWWHSAPRIRSVRHGRDRVALRSAAELSRVEWDGKTLRFEARFAKYQWGAVEAL
                     GVSRPTEVRVDGRPIPECPDLAAVSAPAWRYHADIGTLEVRLTRPGSAWVEALGVTHA
                     PVVVVVDRID"
BASE COUNT          268 a          590 c          570 g          249 t
ORIGIN      
        1 gtcaatccgg tcgaccacca cgactacggg agcgtgggtg acgccgagtg cctcgaccca
       61 tgcgctgccc ggccgggtga ggcgcacctc cagagtacca atgtcagcat ggtaccgcca
      121 agcgggtgcg ctcacggcgg cgagatccgg acactccgga atcggtctgc catcgactcg
      181 cacctcggtg ggccgactca cgcccagagc ctcgacggca ccccactggt acttcgcgaa
      241 ccgggcctcg aagcgcagcg tctttccgtc ccactcaacc ctcgacagct cggcagccga
      301 ccgaagcgcg acacggtcgc gtccgtgtcg aactgagcgg atccgaggcg ccgagtgcca
      361 ccagttccgg tgtgccaggt tcgcgtagac gagtgtcgtc ggcgtgaagc atggcggagt
      421 ctgaccatgg aggcccggac gcccatggat gaagttcagg gcatcgggcc actgcgccag
      481 gcggtcgccc tcggtcgctt gctggtacat gccactcacg agcaggttct ccgcaacccg
      541 ctcccagggg aacgagtcgt catagcgcgc cagacgacgg agggcctccg cataggccat
      601 gccattccac tgcacagcta ccccgagcca gctcagtatg taggatgtcg ccccgaatat
      661 cgggatgctc gcgccttgca tggccggctg gtccggcgcg ctccagacgt acacgaacgg
      721 cagaccggtt cgtgcccaat aggcagcgtc ggccagatgc gcacggtcgc ccgtcagctc
      781 gtagccgagc aggtaggcgt tcaccgccac cgcggaggcc aggatgtccg gcgagtggac
      841 ggggcactcc cacacttggg cggcccgagg gacgcggaac cggcgcatgg ccctgagtgc
      901 ccgcaggcct gcctcctcgg cctgcgggtc gccggtcagc agtgcgtact cgaggaccgt
      961 ggcggccgac ggtgcggtga gacccacctc tgaggctccc tcggcaccga ggtagtcgta
     1021 gtcgatgacc tcggccgtcc accccgcctt cgcgtgctcg cccgagaacc gccatgtgcc
     1081 atcggcagcc tggttggcga tgagccctcg ggcctgggcc gccaaatcgg cgagggcgcc
     1141 cgccgacgac cggaactcga cggcgtagtc ccgtgccgcc tgatcgccgc ccataacctc
     1201 ggcggccagc gccgcggcct gctcggcctc ggctccgccc gcgaggatgc gagcgccgat
     1261 gagcagttcg tgggcgggtt cgcgattggg gtggaacccg acgatcaggt ccgagtacca
     1321 acagccccac tcggggttcc acagagcgtg ttgcttggcg tagccctgca gtgagaaggc
     1381 cagctctgcc cgggcgtctc ctcggggcat ggcgagcggc tccgggtagc catggagctc
     1441 gaaccaccgg tcgagagccg ccagcgaatc ggccgccccg ggcgcgccca tcaggtcggc
     1501 ttggagcgtg atcgtcagac cgccgccgac cggtatgggt ctctcggctc gcatggcgtt
     1561 ctccggcatg cctatccccg cgcccgggag catcaggccc acgaggtggt tgtcgggccc
     1621 ctgcaggcgg ttcggggagg agaagacaac gctggccggt cccagaccct caggcag
//
LOCUS       DAOSPL010001203         1677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_72451_length_1677_cov_2.887176, whole genome shotgun sequence.
ACCESSION   DAOSPL010001203 DAOSPL010000000
VERSION     DAOSPL010001203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72451_length_1677_cov_2.887176"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..394)
                     /locus_tag="PLQ54_18225"
     CDS             complement(17..394)
                     /locus_tag="PLQ54_18225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95253.1"
                     /translation="MPACPECLYGVGSQDTKCGFCGAELDQAKSIAAEAERFAIVPKP
                     PYMLGESVIPAVLVLGGIAAVAAGAVFAMQAMGAEMDTNQVGRLTVMVSNGAMCLIFG
                     LWSIGRQRARQRVFSSPPTRRAR"
     gene            546..>1677
                     /locus_tag="PLQ54_18230"
     CDS             546..>1677
                     /locus_tag="PLQ54_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOS95254.1"
                     /translation="MKAIMVMFDSLNRHMLRPYGCDWVHTPNFQRLADQAVTFDRSYV
                     GSMPCMPARREIHTGRYNFLHRSWGPIEPFDDSMPELLKTSGVHTHLVSDHYHYWEEG
                     GCNYHTRYSTWEASRGQEGDPWKAHLSHIDLPERMHDHGVWGRQDLINRHYMQREEDQ
                     PQAKTFAGGLEFLYTNATADNWFLHIETFDPHEPYFSPERFHRLYPEAYDGPEFDWPP
                     YQRVAESPEQVAHCCRRNAALVSMCDAYLGRVLDTMDELGLWDDTLLIVNTDHGFLLG
                     EHGWWGKCAQPFYNEIAHTPLFVWDPRCRCEGERRDALVQTIDLPATLLEYFGQALPP
                     DMQGKPLREAIARDAPVREAALFGLHGAHVNCTDGRYVYMRAP"
BASE COUNT          323 a          569 c          524 g          261 t
ORIGIN      
        1 gccgccggga tgcacgctac ctcgcgcggc gagtcggcgg cgacgagaag acgcgctgac
       61 gcgcccgctg acgcccgatg gaccagaggc cgaaaatgag gcacatggct ccgttggaga
      121 ccatgacggt gagccggccc acctggttgg tatccatctc tgcgcccatg gcctgcatgg
      181 cgaaaacggc acccgcggcg acggccgcga tgccgccgag cacgagaacc gcggggatga
      241 cgctctcgcc gagcatgtac gggggcttgg gaacaatcgc gaaccgctcg gcctccgccg
      301 caatggactt ggcctgatcc agttcggcgc cacagaaccc gcacttggtg tcttgggacc
      361 ccacgccgta cagacactcg gggcacgccg gcatacactc acaccctcgc acgcccaaag
      421 ggcgcaatgt ggacacctca tgatacccgg ccgttcgagg catgccaagc cggacgccga
      481 cgcttcggga ggccccgccg agcactcacc cgaaggtcaa gagacgtgaa tggaggccct
      541 gctcaatgaa ggccatcatg gtcatgttcg actcgctgaa ccggcacatg ctacggccct
      601 atgggtgcga ctgggtccac acccccaact tccagcgtct ggctgaccag gcggtcacct
      661 ttgaccgcag ctatgtgggc tccatgccct gtatgccggc cagacgcgag atccacacgg
      721 ggcggtacaa cttcctccac cgaagttggg gacccatcga gccgttcgat gactccatgc
      781 ccgagcttct caagacgagt ggcgtccaca cccacctcgt cagcgatcac taccactact
      841 gggaggaagg cgggtgcaac taccacaccc gctacagtac ctgggaggct tctcggggcc
      901 aggagggcga cccctggaag gcacacctta gccacattga cctgccggag cgcatgcacg
      961 accacggcgt ctgggggcgg caggatctga tcaaccggca ctacatgcag cgagaagagg
     1021 accagccaca agccaagacc ttcgccggcg ggcttgagtt cctgtacacc aacgccaccg
     1081 ccgacaactg gtttctgcac atcgagacct tcgaccccca cgaaccctac ttcagtcccg
     1141 agcggtttca tcgcctctat cccgaggcat acgatgggcc ggagtttgac tggccaccct
     1201 atcagagggt cgccgagagt cccgagcagg tggcgcactg ctgccgccgc aacgcggcac
     1261 tggtcagtat gtgcgacgcg tatctcggcc gcgtgctgga cacgatggac gaactgggcc
     1321 tgtgggacga cacgttgctg atcgtgaaca ccgaccatgg gttcttgctg ggcgagcacg
     1381 gctggtgggg gaagtgcgcc cagccgttct acaatgagat cgcgcacacg ccattgttcg
     1441 tgtgggaccc gcgatgccgg tgtgaggggg agcggcgcga cgccctggtg cagaccatcg
     1501 acctacccgc caccctgctc gagtacttcg gccaggcact gccgccagac atgcagggca
     1561 agcccctgcg ggaggccatc gcccgcgacg ccccggtgcg ggaggcggct ctgtttggtc
     1621 ttcacggcgc gcatgtcaac tgcacggacg gccggtatgt ctacatgcgc gcacccg
//
LOCUS       DAOSPL010001204         1677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_72472_length_1677_cov_2.053021, whole genome shotgun sequence.
ACCESSION   DAOSPL010001204 DAOSPL010000000
VERSION     DAOSPL010001204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72472_length_1677_cov_2.053021"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PLQ54_18235"
     CDS             complement(<1..406)
                     /locus_tag="PLQ54_18235"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOS95255.1"
                     /translation="MSSSCRPVCLAAPVLVLACALAPADQEVRYGVGSWPEAGHGNHR
                     AVVRADGPAPAVWAHIDWRRRDRDPEAKDVRVVDLTTGQAVTNVVRAHVGRESGDIIF
                     EPATAPGDYGIYYLPYNPGTGNFDAAGTYFAPK"
     gene            complement(496..945)
                     /locus_tag="PLQ54_18240"
     CDS             complement(496..945)
                     /locus_tag="PLQ54_18240"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOS95256.1"
                     /translation="MNPAEVRQAIAALLSSQRLGVLATQDGGQPHTGLVAFATVPDLS
                     SLVFATDRDTRKYRNLKADPRASMLVDNRENLPADLRHAVSVTANGTCGELAGADRAR
                     ELDRLLTRHPSLAAFLGGASTAVFLLEVSAYSFVSHFSAVQGFVPSP"
     gene            1213..>1677
                     /locus_tag="PLQ54_18245"
     CDS             1213..>1677
                     /locus_tag="PLQ54_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOS95257.1"
                     /translation="MHRRLCWIALAVLVAASLPGCKSKPEPEPAAAPAAGQTTSSTAP
                     AVHPADVTIGFLVKQPEEPWFQNEWKFADKCAKEYGFRLSKMGAENGEKVLANIDALH
                     TQGAQGFIICTPDVKLGPAIMAKAKQYGMKVIAVDDQFVDKGYNGIAGNLDRL"
BASE COUNT          287 a          569 c          557 g          264 t
ORIGIN      
        1 cctttggcgc gaagtaggtg cccgcggcgt cgaagttccc ggttcccggg ttgtatggaa
       61 ggtagtagat cccatagtca cccggcgccg tcgcaggctc aaagatgata tcgcccgact
      121 cccggccgac atgggcgcga accacattgg tgacggcctg cccggtagtg aggtcgacca
      181 cccggacgtc cttggcctcc gggtcgcggt cccgccggcg ccagtcgatg tgcgcccaca
      241 ccgccggggc cgggccatcg gctctcacga cggcccggtg gttcccgtgc ccggcctcag
      301 gccacgatcc gacgccgtag cgcacctcct gatcggccgg cgcaagagca caggccagca
      361 ccagcacggg ggcggcaaga cagacaggtc tacacgaact cgacacggct cattcctccg
      421 actacgcccg ggagcgtcac tgccgagcta gttcgccgct ggccggacga ggccttggcc
      481 gaccccgagc tcgcctcacg ggctggggac gaacccctgc accgccgaga agtgcgagac
      541 gaaggagtag gccgacacct ccagcaggaa caccgctgtg ctagccccgc ccaggaacgc
      601 cgccaggctc ggatggcgcg tgagaaggcg gtcgagctca cgcgctcgat ccgcccccgc
      661 gagctcgcca cacgttccgt tcgcggtaac tgacaccgcg tggcgcagat cggccggtag
      721 gttctctcgg ttgtccacta gcatcgatgc ccgcggatca gccttgaggt tgcggtactt
      781 gcgggtgtca cggtcggtgg cgaacacgag cgagctcaag tcgggcaccg tggcgaaggc
      841 gaccaacccc gtatggggct gcccgccatc ttgggtggcg agtactccca ggcgctgcga
      901 agagagcagg gctgcaatcg cttgtctgac ttcggccggg ttcatggtta tgcgctccct
      961 cagtcccatg gtcccgcgga ctgcacgcgt agcgggtgtc tctaccatca acgtcgggca
     1021 cggtggctgg ggctccgccc tgcactgcct ccggccgcat cgggaggttg tcgccgcgcc
     1081 gcagccgaag aatcctttgg cgtttcgcta cagccccggc cacccaggca ttggggctgt
     1141 cgagttgcga gctgtcagag agccggcttc actcggcctg cgcgagtgtc gcctgaggag
     1201 gcttcattgc ccgtgcatag acgactgtgc tggatcgcac tcgccgttct cgtcgcggcc
     1261 tcactccccg ggtgcaagtc gaagcccgag ccggagcccg ccgctgcacc ggccgcgggc
     1321 cagaccacgt cgtcgaccgc ccctgcagtc cacccggcgg acgtgaccat cgggttcctg
     1381 gtgaaacagc ccgaagagcc gtggttccag aacgagtgga agttcgccga caagtgcgcc
     1441 aaggagtacg ggttccggct cagcaagatg ggcgccgaga acggggagaa ggtgctcgcg
     1501 aacatcgacg cgctccacac gcagggggcc caggggttca tcatctgcac tcccgacgtg
     1561 aagctcgggc cggccatcat ggccaaggcc aagcagtatg gtatgaaggt catcgccgta
     1621 gatgaccagt tcgtcgacaa gggctacaac gggatcgcgg gcaacctcga caggctg
//
LOCUS       DAOSPL010001205         1676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_72506_length_1676_cov_3.067859, whole genome shotgun sequence.
ACCESSION   DAOSPL010001205 DAOSPL010000000
VERSION     DAOSPL010001205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72506_length_1676_cov_3.067859"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1083)
                     /locus_tag="PLQ54_18250"
     CDS             complement(94..1083)
                     /locus_tag="PLQ54_18250"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS95258.1"
                     /translation="MQKRVLGRTGLMVGELSLGAAFITGGPDGARGATPVVRRALELG
                     MNLVDTSADYGDSEAALGAALDELGQPFIVSTKLGPRGRGFDPKDKDALRRAVDESLK
                     LLRRDTLDILMIHEPDRPGEIDWYSDTTSFHGPVTELLQELKDRGVVRFTGLGGTTVY
                     QMAMLVATGEYDVVLTAFNSSPLWREALTHVIPEAAKQNMGVLLASPTQQGWLARRFD
                     DEVRHGARWLSPQRRAQFLALYDLLDETGIEIPVFCLRWALGVPGVSTVLTGPRTVEQ
                     LEQNFRAVEEGPLPADVVARLDEIAAMVPYRPFEEPFGCMLGNPHYWGPGPAR"
     gene            1376..>1676
                     /locus_tag="PLQ54_18255"
     CDS             1376..>1676
                     /locus_tag="PLQ54_18255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95259.1"
                     /translation="MRLLLTSMIAGSLWSAPVGAGPDAQLVGPLTFEPATPAPIVSPG
                     LPGPAGAPLDLRPAPAGPFDGSSGSAPCRRPAADGALYWAGVDHSAQAAEMLETAA"
BASE COUNT          258 a          600 c          548 g          270 t
ORIGIN      
        1 cgggatgtcc atcatgccca gcacgcccgg agcgttcgcg cacacggctc tcatgggggt
       61 cagcctcccg tagtcggtca accgggccgt gactcagcgc gcggggcccg gtccccagta
      121 atgtgggttg ccgagcatgc acccgaaggg ctcctcgaaa ggtcgatagg gcaccatggc
      181 ggcgatctcg tccagacgcg cgaccacatc ggccggcagt gggccttcct cgacggcgcg
      241 aaagttctgc tcgagctgct cgacggtgcg tggcccggtg agcacggtgg agacgcccgg
      301 cacgcccagg gcccaacgga ggcagaatac cgggatctcg atccccgtct cgtccagcag
      361 atcatagagc gccagaaact gcgctcggcg ttgcgggctc agccaccgcg caccgtggcg
      421 cacctcgtcg tcgaagcgtc gcgcgagcca gccctgctgg gtcggtgagg ccagcaggac
      481 gcccatgttc tgcttggccg cctctgggat cacatgcgtc agtgcctcgc gccacagcgg
      541 gctgctgttg aaggcggtga ggaccacatc gtactccccc gtcgccacca gcatggccat
      601 ctggtaaacc gtggtgccgc ccagacccgt gaaccggacc accccgcgat ccttgagctc
      661 ctgcagcagc tcggtcaccg gcccatggaa ggacgtggtg tccgaatacc agtcgatctc
      721 gcctggccgg tcgggctcgt ggatcatcaa gatgtcgagc gtgtcgcggc gcagcagctt
      781 gaggctctcg tcgacggcgc gccgcagcgc gtccttgtcc ttgggatcga atcctcggcc
      841 gcgaggacca agcttggttg acacgatgaa cggttgtccg agctcatcaa gtgccgctcc
      901 cagggcggcc tcgctgtcgc cgtagtccgc cgaggtgtcg acgaggttca tcccgagctc
      961 caaggcccgc cgcacaaccg gggtggctcc ccgggcaccg tcgggcccgc cggtaatgaa
     1021 cgcggcacca agggacagct cgcccaccat caggccggta cggcccaaga cacgcttctg
     1081 catcccagtt cctccatggt gcggccaatc gcccccccgc ggcgcagggg cccgcaccgt
     1141 ttccgctgtt gcccaccggt ggccttctgt gggaacaccg cagacatggc aggccggaca
     1201 cccgtgccac cgcgcgcacc aaatcggtgt cattgttgac aatcacagtg tgttgaggca
     1261 tgatctcccc tggagtgcgt ggacctatgc cgtcccgtgt ccgttcggcc cggtccctgg
     1321 ctgcggtgag cgccgacacg cggtgcgtga gcgcaaagca cggggggatc cggacatgcg
     1381 actgctgctg acctcgatga tcgcaggtag cctgtggtct gctccggtcg gagcgggccc
     1441 cgatgcccag cttgtgggcc ccctcacatt cgagccggcc acaccggcac cgatcgtctc
     1501 gcccggtctg cccggcccgg caggtgcccc actcgatctg agaccggccc ccgcggggcc
     1561 cttcgacggt tcgtccggtt ccgccccttg ccggcgaccg gcggcggacg gcgcgctcta
     1621 ctgggccggc gtcgaccatt ccgcccaggc cgccgaaatg ctcgaaacgg cggcga
//
LOCUS       DAOSPL010001206         1676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_72509_length_1676_cov_2.837138, whole genome shotgun sequence.
ACCESSION   DAOSPL010001206 DAOSPL010000000
VERSION     DAOSPL010001206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72509_length_1676_cov_2.837138"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PLQ54_18260"
     CDS             <1..500
                     /locus_tag="PLQ54_18260"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOS95260.1"
                     /translation="AELATQAAALDDLGAALAERRMFLGIHTHSPEMSHNAREFRYNL
                     DHTDPAKVGLCADFHWIYRGGADPYAITERYAGRIVTTHLRNSLQAVWCECFCRGDLD
                     YGRIEESLLAVRYRGPLVIEIALEPRTPRTRGPLENLRASREYLAQCLGRVWSGKSYT
                     RDYNP"
     gene            687..>1676
                     /locus_tag="PLQ54_18265"
     CDS             687..>1676
                     /locus_tag="PLQ54_18265"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOS95261.1"
                     /translation="MSLVGLDVGTTGTKAVVVDSRGRILGESYREYPLYTPQPGWNEL
                     DPVEVWSAVRTVLGEAVSRAGAADPVRAIGISSQGEAGVALATDGGILARSQVSFDQR
                     STPQCERWAADFGAERTFEITGQPVHPMYTLPKLIWMRENEPGFAERLWKFLCFEEYV
                     IYQLTGELAASWALASRTMAFDVRRKCWSKEILDAAGFSEAIFAAPLPSGTPVARVRP
                     EVADELDLPSDTIVATAGHDQPCGALGAGIVRAGVAVDGTGTVECITPAFSEPVLNET
                     MRRNSFCCYPHTVRDLYVTLAFCFAGGSLLRWFRDTLGAGEMAEARRTGHDVYD"
BASE COUNT          259 a          616 c          529 g          272 t
ORIGIN      
        1 acgccgagct cgccacccag gcggcggcgc tcgatgacct gggcgccgcc ctggcggaac
       61 gccgcatgtt tctgggcatc cacacccact cgcccgagat gagccacaac gcccgcgagt
      121 tccgctacaa cctcgaccac accgatccgg ccaaggtcgg actatgtgcc gacttccact
      181 ggatctaccg cggtggcgcc gatccctatg ctattaccga acgctacgcc ggccgcatcg
      241 tcaccaccca cctgcgcaac tcgctccagg ctgtctggtg tgagtgcttc tgccggggcg
      301 acctcgacta cgggcgcatc gaggagtcgc tcctcgcggt gaggtaccgg ggccccctcg
      361 tcatcgagat cgccctcgag cctcgaacgc cgcgcacgcg tggcccactc gagaacctcc
      421 gcgccagccg cgagtacctg gcccagtgcc tcggccgcgt ctggtcgggt aagagctaca
      481 cacgcgatta caacccttag acgccatgcg gggtgtcaag ccccttcgca acgcgggccg
      541 ccgcgaaagg cgctcagacc ccctcccgcc cctttgaccc tgcccatgcc ctgtgctaga
      601 ctcgccaccg tccgccgggg gcgtttggac gccggtccgg cgcgccccga cggggcgccg
      661 atgtgacacc tcagggtgac ccccgaatga gtctcgttgg cctggatgta ggcaccaccg
      721 gcaccaaggc cgtcgtggtc gactcccgcg gccggatcct cggcgagtcc taccgcgagt
      781 accctctcta taccccgcag cccggctgga acgagctcga tcccgtcgag gtctggagcg
      841 ccgtccgcac ggtgctgggc gaggccgtct cccgggcggg cgccgctgat ccggtccgcg
      901 ccattggcat ttcgtctcag ggcgaggccg gggtggcgct ggccaccgat ggcggcatcc
      961 tcgcccggag ccaggtgagc ttcgaccaac gcagcacgcc ccagtgcgag cgctgggccg
     1021 ccgactttgg cgccgaacgc accttcgaga tcaccggcca gccggtgcat cccatgtaca
     1081 cgttgccgaa gctcatctgg atgcgcgaga acgagcccgg cttcgccgag cggctctgga
     1141 agttcctgtg cttcgaggag tacgtgatct accaactgac tggcgagctc gcggccagct
     1201 gggcattggc ctcacgcacc atggccttcg acgtccgccg caagtgctgg tcgaaggaga
     1261 tactcgacgc cgcggggttc tccgaggcca tcttcgccgc accactgccg tcaggaacac
     1321 cggtggcacg cgtgagaccc gaggtggccg acgaactcga tttgcctagt gatactatcg
     1381 tcgccacggc cgggcacgac cagccctgtg gggccctcgg cgccgggatc gttcgggcgg
     1441 gcgtcgccgt cgatggcacc ggcaccgtcg agtgcatcac gcccgccttc tccgagccgg
     1501 tgctcaacga gaccatgcgc cgcaatagct tctgctgcta tccacatacc gtacgagatc
     1561 tctacgtcac gctggcgttc tgcttcgccg gcgggtcgct actgcggtgg ttccgtgaca
     1621 cgctgggcgc cggggagatg gccgaagccc gccggaccgg gcacgatgtc tacgac
//
LOCUS       DAOSPL010001207         1674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_72603_length_1674_cov_3.007412, whole genome shotgun sequence.
ACCESSION   DAOSPL010001207 DAOSPL010000000
VERSION     DAOSPL010001207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72603_length_1674_cov_3.007412"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..608)
                     /locus_tag="PLQ54_18270"
     CDS             complement(114..608)
                     /locus_tag="PLQ54_18270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95262.1"
                     /translation="MRRPRSDRQAAIREPRRTLLTKPQALAPVVTATTSSPVNGPRSQ
                     RASGVDVGHIVLRESSAREMRAPGHGDTPQASLIELVHEAISEALASLEIWAVRGYLV
                     KAWQEPQRHSWIAECPTVGCVVEQDSIDAARAAIADSIQDMLEALESEGHPRPPRDLD
                     AANG"
     gene            complement(595..918)
                     /locus_tag="PLQ54_18275"
     CDS             complement(595..918)
                     /locus_tag="PLQ54_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95263.1"
                     /translation="MAEPKGRTRGAAQAAPERRVVAAPHPQPSQYANHVAVLAAGTGD
                     SGEVTFRFYQVSADDLASGGPEVTALPLGSIVMQRGFAEDFVRNAAQTLGIGLEGEAK
                     ADATP"
     gene            complement(1191..>1674)
                     /locus_tag="PLQ54_18280"
     CDS             complement(1191..>1674)
                     /locus_tag="PLQ54_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95264.1"
                     /translation="NGNGGWDYPGDACCIERDRWANMMQPYIKNQQIVYCPAAANDVP
                     QWGGRTWNPPVSYKYKHACATNAWKDTGIAFPAQVYMLMEYKNWHSGTLQCACSTPTG
                     DQMHNMVFFDGHSKVMSKSRAARIVYGGATYWDPHWLQNPNNPAEQTADAGIGVDFMQ
                     "
BASE COUNT          266 a          600 c          513 g          295 t
ORIGIN      
        1 ttgggtcatc ggggtggatg tagacggccc cgtgcctcgc ctggcgcacg aggcagaacc
       61 tattgcggcg caggatggcc gccacttcct gtgacgatcg gggcacgatc cggctaccca
      121 ttcgcggcgt ccagatccct tggcggacgc gggtgcccct cactctcgag ggcctcgagc
      181 atgtcctgga tggaatcggc gatcgctgct ctggccgcgt cgatggagtc ctgttccacg
      241 acgcaaccaa ccgtggggca ctccgcgatc catgagtgcc tctggggctc ctgccacgcc
      301 ttgaccaagt agccccgcac agcccatatc tcgaggctcg ctagcgcctc ggagatcgcc
      361 tcgtgcacga gctctatcag tgacgcctgg ggcgtgtcgc catgcccggg ggcgcgcatc
      421 tcccttgccg atgactcccg aaggacgatg tgcccgacat cgaccccaga cgcccgttgc
      481 gagcgcgggc cgttgaccgg cgagctcgtg gtggcggtca cgaccggtgc tagcgcctgc
      541 ggcttcgtga gcagggtgcg acgcggctct cggatcgctg cctggcggtc agacctaggg
      601 cgtcgcatcc gctttcgcct ccccctccag gcctatgccc agggtctgcg cagcattccg
      661 cacaaagtcc tcagcgaacc cgcgctgcat gacgatggat ccaagcggca gggcagtcac
      721 ctcagggccc cccgatgcca ggtcgtcggc tgagacctgg tagaagcgaa acgtgacctc
      781 gcccgaatcc cccgtccctg cggcgaggac tgccacatgg tttgcatact ggctaggctg
      841 ggggtgtggc gcggcgacta cccgccgctc cggcgccgcc tgtgcggccc cccgggtccg
      901 gcccttcggc tcggccatcc cagatcaact cctctggcac cggtcagcta gggcgaacgg
      961 gtcactggcg agaggcccct cgttcctacg ataccacacc aagtgtcccc gctcgcccca
     1021 cgatcgcctc atcggcatcg ccagggcccg acgggggcaa cacacaggcg gcgtcctcga
     1081 gacgctacca ccccaccccg ccacagatgc tacactcagc agccccagac acacagaggg
     1141 cggagcgccg ccgcgcgctc cgccccgcca tgcgcagcaa taccgaagac ctactgcatg
     1201 aagtccacgc cgatcccggc gtccgcggtc tgctccgcgg gattgttcgg attctgcagc
     1261 cagtgcgggt cccagtacgt cgcgccacca tatactatcc gcgccgcacg gctcttgctc
     1321 atgaccttcg aatggccatc aaagaacacc atgttgtgca tctggtcgcc cgtcggcgtg
     1381 ctgcacgcac actggagagt cccggagtgc caattcttgt actccatcag catgtacacc
     1441 tgtgcgggaa acgcgatgcc tgtgtccttc cacgcattgg tggcgcacgc atgcttgtac
     1501 ttgtagctca caggcgggtt ccaggtgcgg ccgccccact gagggacgtc gttcgcggcg
     1561 gcggggcagt agacgatctg ctggttcttg atgtacggct gcatcatgtt cgcccaccga
     1621 tcgcgctcaa tgcagcaggc gtcgcccggg tagtcccagc ctccgttccc gttc
//
LOCUS       DAOSPL010001208         1673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_72673_length_1673_cov_2.408529, whole genome shotgun sequence.
ACCESSION   DAOSPL010001208 DAOSPL010000000
VERSION     DAOSPL010001208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72673_length_1673_cov_2.408529"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="PLQ54_18285"
     CDS             <1..1081
                     /locus_tag="PLQ54_18285"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOS95265.1"
                     /translation="AGVIKALSPFVLMAGHHRSFLEIHLRKTLQTSRGYGTNVPHIVS
                     TSFLTHEPIAEHLERHGNHGYEGSVYLSPGRAIGQRMIPMVRDLVCLWEETAQEALDE
                     QKQKVRDAVRAALVRWAKEKGEGSDYVDNIPAQRFHPPGHWYEVPNLLRNGVLARLLE
                     EHPKLTTIMLHNIDTLGADLDPAALGAHLASGNALTFEVVPRRANDRGGGLARVNGRI
                     RLLEGLAQPRDGDELRLRYYNSGTCWIEIDPLLDLFGLNRNDLRGPEERLTEAVRHLA
                     GRVPTYVTIKDVKWRWGHGQEDVYPTAQFEKLWGDMTALADVRCGYLAVPRRRGQQLK
                     DPSELDPWANDGSKAHVARLAGLEG"
     gene            complement(1100..1393)
                     /locus_tag="PLQ54_18290"
     CDS             complement(1100..1393)
                     /locus_tag="PLQ54_18290"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOS95266.1"
                     /translation="MDDILYCPHRPDEACACRKPRPGLILEAIARYEVDPASSFMIGD
                     REGDMAAGRAAGVRTIRIVTRLTGADAAHTQAEHQAPDLPTAVDYILGAASVK"
BASE COUNT          296 a          569 c          557 g          251 t
ORIGIN      
        1 cgcgggcgtc atcaaggccc tcagcccctt cgtactcatg gccgggcacc accggagctt
       61 cctcgagatc cacctccgca agacactcca gacgtcccga ggctatggga cgaacgtgcc
      121 acacatcgtg tcgaccagct tcctcacgca tgagcccatc gccgagcacc tcgagcgcca
      181 cgggaaccac ggctatgagg gatcggtgta cctgtcgccg gggcgagcaa tcggccagcg
      241 gatgatcccc atggtgcgcg acctggtgtg cctctgggag gaaacggccc aggaggctct
      301 ggacgagcag aagcaaaagg tgcgagatgc cgttcgcgca gccctggtca ggtgggccaa
      361 ggagaagggt gaggggagtg actatgtgga caacatccct gcccaacgat tccacccacc
      421 aggacactgg tatgaggtac cgaatctgct gaggaacggc gtgctagcgc ggctgctgga
      481 ggagcacccg aagctcacca ccatcatgct ccacaacatc gacacgctgg gggccgacct
      541 cgatccggcg gccttgggcg ctcacctggc atcaggcaac gcattgacct tcgaggtggt
      601 gccgcgacgt gccaacgatc gcggcggcgg gttggcgcgg gtgaacgggc ggattcgtct
      661 gctcgaaggg ttggcccagc cacgcgacgg ggacgagctg cggttgcgct actacaactc
      721 gggcacctgc tggatcgaga tcgatccgct gctcgacctc ttcgggctga accgcaacga
      781 tctgcgcggg ccggaagaac ggctcactga ggccgtgcgt cacttggccg ggcgcgtgcc
      841 gacctatgtg acgatcaaag acgtgaaatg gcgctggggt cacggccagg aggatgtgta
      901 ccccacggcc cagttcgaga aactctgggg cgatatgacc gcgctggccg acgtgcgctg
      961 cgggtacctg gcggtccctc gccgccgggg acagcagctg aaggatccca gcgaactgga
     1021 cccatgggcc aacgacggta gcaaagcaca cgtggcgcga ctggccggac tggagggcta
     1081 gcccggcccg tgaccgcctc tacttcacgc ttgccgcacc gaggatgtaa tccaccgccg
     1141 tgggcaagtc cggcgcctgg tgttcggcct gggtgtgggc cgcgtcggca cccgtcaggc
     1201 gagtcacgat acggatggtc cgcacgccgg cggcgcggcc cgccgccatg tcgccctcgc
     1261 gatcgccgat catgaatgac gatgccggat cgacctcgta ccgcgcgatg gcctccagga
     1321 tcagaccggg ccgcggtttg cggcaggcgc acgcctcgtc gggccggtgg gggcagtaca
     1381 ggatgtcatc cagctcgccg ccccccgtcg ccctttcccc ccgccccgat ggacccgcgg
     1441 ccgaccgatc aggaacgtct acgcctgggg aggctcacgg cccgtcggag gaccgcaatg
     1501 agcgcacccg aggctcttac ccccctgagg ccagctgcta ccgctgggcc ccaaacgacc
     1561 tccgggcgga tcgccagacc ctgggcaagc ggacgagctc cttcgactct gcggcccatg
     1621 gacgcccatg cccggtcgct actcgctcac caggatccct ggctggtgtg cac
//
LOCUS       DAOSPL010001209         1672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_72705_length_1672_cov_3.845393, whole genome shotgun sequence.
ACCESSION   DAOSPL010001209 DAOSPL010000000
VERSION     DAOSPL010001209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72705_length_1672_cov_3.845393"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /gene="priA"
                     /locus_tag="PLQ54_18295"
     CDS             <1..1265
                     /gene="priA"
                     /locus_tag="PLQ54_18295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOS95267.1"
                     /translation="NLGLLVIDEEHETSYKQDRAPRYHAREVARRRAERAGAVLVLGS
                     ATPSLETTYEANQGRCRRVVLPERVGGGTLPTVDIVDMRQEKADQRRTMLGSRLVEEI
                     GARLERGEQTILFLNRRGYSPFIMCRQCGHAWRCPECDVSLTFHMEEKVLRCHHCDWS
                     AAAPTECDQCGSIHVAFFGTGTERVEQEVSELFPQARLLRMDRDTVTSKGAHARILAQ
                     FARGDADILMGTQMIAKGLDFPEVTLVGVVLADTGLGFPDFRAAEHTFQLLSQVSGRS
                     GRGAKAGRVLIQTFRPDHYAVVCAAQHDYDGFAERELRSREPFGYPPFRRLANLVTSD
                     DAEARAEADATACAEAFENVARSAESVTVLGPAPAPLRRLRGRFRWHVEVLAPRDALG
                     AVVDEALGRLDAAVRDRVVVDIDPVSTL"
     gene            1285..>1672
                     /gene="def"
                     /locus_tag="PLQ54_18300"
     CDS             1285..>1672
                     /gene="def"
                     /locus_tag="PLQ54_18300"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOS95268.1"
                     /translation="MAVLPIVVYGDPVLRVKARPVARVDAEIRQLLADMHETMVQAPG
                     IGLAANQVGRPVRCLVASVDDSNLLMVNPRIVSRKGSYEAIEACLSLPGLQATVPRAE
                     RVVVAGLNRNGKPTKVEAEGLLARCLQ"
BASE COUNT          252 a          531 c          608 g          281 t
ORIGIN      
        1 agaacctggg ccttttggta atcgatgagg aacacgagac atcgtacaaa caggaccgcg
       61 ccccgcggta ccatgcccgc gaggtggccc ggcggcgcgc cgagcgggcc ggggccgtct
      121 tggtgctggg tagtgccacc ccgtcacttg agacgaccta tgaagccaac cagggccgtt
      181 gccgccgcgt ggtgctgccg gagcgggtgg gcggcgggac tctccccacc gtcgacatcg
      241 tcgatatgcg ccaggagaag gctgatcagc ggcgtaccat gctcgggagt cggctggtcg
      301 aggagatcgg cgctcggctt gagcgcggcg agcagaccat cctattcctg aaccgccggg
      361 gctactcgcc tttcatcatg tgtcgccagt gcggccacgc gtggcgctgc cccgagtgcg
      421 atgtctcgct caccttccac atggaggaga aggtactccg ctgccaccac tgcgactggt
      481 cggcggcggc gccgaccgag tgcgaccagt gcgggagcat ccacgtggcg ttcttcggga
      541 ccggcacgga gagggtcgag caggaggtga gcgagctttt cccccaagct cggttactcc
      601 gcatggaccg cgatacggtg acctcgaagg gcgcccacgc ccgcattctt gcccagttcg
      661 ctcggggtga tgccgacatc ctcatgggaa cgcagatgat cgccaagggg ttggatttcc
      721 cagaggttac cctggtgggt gtggtgctgg cggacacggg gttgggcttc ccggacttcc
      781 gcgcggcgga gcatacgttt cagctcctct cacaggtgag cggccggtcg ggccgcgggg
      841 ccaaagcggg ccgggtactg atccagacct tccgcccgga ccactacgcg gtggtgtgcg
      901 cggcccagca tgattacgat gggttcgcgg agcgggaact gcgatcgcgc gaaccttttg
      961 ggtaccctcc cttccgacgg ctcgcgaatc tggtcacgag cgatgatgcc gaagccaggg
     1021 ctgaggcgga cgccacggcc tgcgcggagg ctttcgagaa cgtggcgcgt tcggccgaga
     1081 gcgtcacggt gctgggcccg gcccccgccc cgctgcggcg cctccgcggc cggttccgtt
     1141 ggcacgtcga agtgctggcc cctcgggacg cgttgggcgc ggtcgtggac gaggcgctgg
     1201 gccgcctgga cgcggccgtc cgagatcggg tcgtggtgga catcgatcca gtgtcgaccc
     1261 tgtgaagagg tggaatcggc tcgcatggct gtcttgccca ttgtggtgta tggcgacccg
     1321 gtgctgcggg tcaaggcgcg gcccgtggcc cgggtcgacg ccgagatccg gcagttgctg
     1381 gccgacatgc acgagaccat ggtccaggcg ccgggcattg gcctggcggc gaaccaggtg
     1441 gggcgtccgg tgcggtgctt ggtggcctcg gtcgatgaca gtaacctctt gatggtcaac
     1501 ccgcgcatcg tgagccgcaa gggctcctac gaggccatcg aggcgtgcct cagcctgccc
     1561 ggcctgcagg cgaccgtgcc tcgcgccgag cgtgtagtgg tcgccggtct gaaccgcaac
     1621 gggaagccca ccaaagtgga ggccgagggc ctactcgcgc ggtgcctgca ac
//
LOCUS       DAOSPL010001210         1672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_72711_length_1672_cov_3.382189, whole genome shotgun sequence.
ACCESSION   DAOSPL010001210 DAOSPL010000000
VERSION     DAOSPL010001210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72711_length_1672_cov_3.382189"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PLQ54_18305"
     CDS             complement(<1..766)
                     /locus_tag="PLQ54_18305"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOS95269.1"
                     /translation="MGMCRATMAGFVMLAVAAGCGGRREAAPQTTATGGSATHATEPL
                     VVRVGYFPNITHAPAVLGFSAARNGFAKALAGKAALETKIFNAGPSAMESLHARAIDL
                     CFVGPTPAINAYAKGGDVVLVSNVANGGSVLVARPDVAIAGVSDLAGKKVAVPQIGNT
                     QDAMLRMLLLDAGIQTADKGGATRVVAVENPDVLTLFQTRQLDAACVPEPWGARLERE
                     AGAKVVLDWRAIWRDGNYPVTVLVARKEFLEQHRDVV"
     gene            974..>1672
                     /locus_tag="PLQ54_18310"
     CDS             974..>1672
                     /locus_tag="PLQ54_18310"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOS95270.1"
                     /translation="MRVRQRISLSFGVKTDVGIAREENEDAFDVVVPEPGALWDSRGA
                     LFVVADGMGGHAAGAVASQLAVNAVAAEYYESQHSSVEEALRSGFHRANTEILLSACG
                     DPRRAGMGCTLVVAAVHEALTVANVGDSRAYILHDGHVEQITEDHSLVEAQVRAGLMS
                     REAARESPYRSAVTRCIGSRESADPDLYARELVPGDVVLLCTDGMCDALTDADIARIL
                     GENASPTVSARQLVS"
BASE COUNT          262 a          570 c          560 g          280 t
ORIGIN      
        1 cgacgacgtc ccggtgttgt tcgagaaact ccttgcgcgc cacgagcaca gtaaccgggt
       61 agttgccgtc gcgccagatg gctcgccagt cgagcaccac cttggcaccg gcctcgcgtt
      121 cgagtcgtgc cccccagggc tcgggcacac acgccgcgtc gagttgccgg gtctggaaca
      181 gcgtcaggac gtccggattc tcgacagcca cgacccgcgt ggcccccccc ttgtcggccg
      241 tctggatgcc ggcgtctagc agaagcatcc gcagcatggc atcctgggtg ttgccgatct
      301 gcgggaccgc caccttcttg cccgccaggt cagacacccc ggcgatggcg acatcgggcc
      361 gtgccacgag cacgctcccc ccgttggcga cgttcgacac gagcaccacg tcgccgccct
      421 tggcataagc gttgatcgcc ggcgtgggcc cgacgaagca caggtcgatg gccctggcat
      481 gcagcgactc catggccgat ggcccggcgt tgaagatctt ggtttcgagc gctgccttgc
      541 cggcgagggc cttagcgaag ccgttcctgg ccgcagagaa ccccagtacc gcgggggcgt
      601 gggtgatgtt cgggaagtac ccgacacgca caacgagggg ctcggtcgcg tgggtcgcgg
      661 atccaccagt agccgtggtc tggggcgccg cctcccggcg tccgccacac cccgcggcca
      721 cagctagcat cacgaatccg gccatcgtgg ctctgcacat gcccatctca tacactcctc
      781 caacactctc ataggagact cagacagatc atactagctc cggttcgccc gcggcgccag
      841 tggaaaagat gctgagcgcc accatcctcc cgccgatcac tccgcgcggc ggcgcgcttc
      901 cttgcccctt ggcggagtgc tacaatgccg cgcagtgctt tggtgagacc gcggggccgg
      961 gagacgggtg tcggtgcgcg tgcggcagcg gattagcctc tccttcgggg tgaagaccga
     1021 tgtggggatc gcccgcgagg agaacgagga tgccttcgat gtagtggtgc ccgaaccagg
     1081 ggccctgtgg gacagccgtg gcgctctttt cgtcgtggcc gatggcatgg gcggccatgc
     1141 ggccggagcc gtggccagcc agctggccgt gaacgccgtc gccgccgagt actacgagtc
     1201 ccagcattcg agtgtcgagg aagcactgcg ttccggcttc caccgggcca acaccgagat
     1261 cctgctctca gcgtgtggcg atccgcggcg ggcgggcatg ggttgcactc tggtggtggc
     1321 ggcggtccat gaggcgctca ccgtggccaa cgtcggtgac agtcgcgcgt acatcctgca
     1381 tgacggccac gtggagcaga tcacggagga ccactcgctg gtcgaggccc aggtgcgtgc
     1441 cggactcatg tcccgcgagg ccgcgcggga atcgccgtac cgcagcgcgg tgacccgctg
     1501 catcggctcc agagagagtg ccgaccctga cctttacgcc cgcgagctcg ttccgggaga
     1561 cgtggttctt ctgtgcaccg acgggatgtg tgacgccctc acggatgccg acatcgcccg
     1621 gatactcggc gagaacgcgt ctccgaccgt gtcggcccgg cagctcgtgt cg
//
LOCUS       DAOSPL010001211         1672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_72723_length_1672_cov_2.827458, whole genome shotgun sequence.
ACCESSION   DAOSPL010001211 DAOSPL010000000
VERSION     DAOSPL010001211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72723_length_1672_cov_2.827458"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="PLQ54_18315"
     CDS             <1..1354
                     /locus_tag="PLQ54_18315"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOS95271.1"
                     /translation="QNFQFLQAIAPRFALILAGDHVYKMNYGSLIEFHASRAAHVTVA
                     VVETPIDRARKQFGVLELDEDGRVVSFVEKPEDPVPTPHNPRTCFSSMGIYVFNTDVL
                     EDVLVEDAHDPESGHDFGRDILPRIHKSLRVFGYSFIDSATGAPCYWRDVGTLEQYYD
                     ANIDLITPLPALNLYDPTWPIYTYTQPLPAAKFVLADDWGSARTGHAINSLVSNGCII
                     SGATVVGSVISPDVRIDEYSTVANSIVMSGARIGKHVRIVRAIVDKGAVIPDGSVLEA
                     GNVAFNDDYDVTESGIVVVPKKMAVSRIHGRRLRADGTAPAGDGPLGRILVVDDDKSI
                     RMILEEVLEERGYLVYTAEDGPSAVRVAEGKPLDLAFVDIVMAPMEGAEVIQELRRVN
                     PGLRVVVVTSLTDDERLERARALGIADCIYKPFDLRAVVAATRRLIKPPPPPPAEVKI
                     "
     gene            1402..>1672
                     /locus_tag="PLQ54_18320"
     CDS             1402..>1672
                     /locus_tag="PLQ54_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOS95272.1"
                     /translation="MDAVRALNPTTAASEVDRLGRDLAMDAYRRMVLIRTFEERIFDL
                     FGQGWIRGTSHMCVGQEAVAVGACAGLRPEDQVTSNHRGHGHFLAK"
BASE COUNT          288 a          551 c          541 g          292 t
ORIGIN      
        1 ccagaacttc cagttcctgc aggccattgc gccccggttc gcgctgatcc tcgctggtga
       61 ccacgtctat aagatgaact acggcagcct catcgagttc catgccagcc gggcggccca
      121 tgtgaccgtg gcggtggtcg agacgcccat cgaccgggcc cgcaagcagt ttggtgttct
      181 tgagctcgac gaggacggtc gcgtggtctc gttcgtggaa aagcccgagg atccagttcc
      241 gacaccacac aacccgcgca cgtgcttctc ctcaatgggc atctatgtct tcaacacgga
      301 tgtcctcgag gacgtactgg tggaggatgc ccacgatccg gagtccggcc acgacttcgg
      361 gcgtgacatc ctgccccgta tccacaagag cctgcgcgtc ttcggctaca gcttcatcga
      421 ttccgccacc ggcgcgccgt gctactggcg tgacgtgggc accctcgagc agtactacga
      481 tgccaacatc gacctgatta cgccgctgcc cgcgctgaac ctgtacgatc ccacgtggcc
      541 catctacacg tacacccagc cgctgccggc cgccaagttc gtgctcgccg acgactgggg
      601 aagcgctcgc accgggcacg ccatcaactc gctcgtgtcg aatgggtgca tcatcagcgg
      661 cgccaccgtg gttggctcgg tgatctcccc cgatgtgcgc atcgatgagt acagcacggt
      721 ggccaactcc atcgtcatga gcggggcccg aatcggcaag catgtgcgca tcgtccgcgc
      781 catcgtcgac aagggagccg tcatcccgga cggcagcgtc ctcgaggccg ggaatgtggc
      841 gttcaacgac gactatgatg tgacggagtc aggcattgtc gtggtgccaa agaagatggc
      901 cgtgtcccga atccacggcc ggcgcctccg ggccgatggc actgcgccgg ccggcgatgg
      961 tccactgggc cggatcctgg tcgtggacga cgacaagagc atccgtatga tcctcgagga
     1021 ggtcctcgag gagcgcggtt acctcgtcta cacggccgag gatggccctt cggcggtgcg
     1081 ggtggccgaa ggaaagccgc tcgatctcgc gttcgtcgac atcgtgatgg cacccatgga
     1141 gggagccgag gtcatccagg agctgcgccg agtgaacccc ggcctgcggg tcgtggtcgt
     1201 aaccagcctg accgatgatg agaggctgga gcgggcccgg gcgctgggca ttgccgactg
     1261 catctacaag cccttcgacc tgcgggccgt ggtcgcggcc acccggcgcc tgatcaagcc
     1321 tcccccgcct cctccggccg aagtcaagat ctgaggtggt cgggtgggca acttgggggc
     1381 ccgtgaggtg tctgacatcg cgtggacgcg gtacgcgcat tgaacccaac cacggcagcg
     1441 tcggaagtgg accggctcgg tcgcgatctc gccatggatg cctaccggcg catggtgctg
     1501 atccgcacgt tcgaggagcg catcttcgac ctcttcggcc agggctggat ccggggcacg
     1561 tcgcacatgt gcgtcggcca ggaggccgtc gccgtgggtg cgtgcgccgg acttcgaccc
     1621 gaagaccagg tcaccagtaa ccaccggggc cacggccact tcctggccaa gg
//
LOCUS       DAOSPL010001212         1670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_72850_length_1670_cov_2.193808, whole genome shotgun sequence.
ACCESSION   DAOSPL010001212 DAOSPL010000000
VERSION     DAOSPL010001212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72850_length_1670_cov_2.193808"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PLQ54_18325"
     CDS             complement(<1..271)
                     /locus_tag="PLQ54_18325"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOS95273.1"
                     /translation="MPFTVKNAEGRIMHSNRYNVCVSGWRSLDDMLGYTSEELYPPDQ
                     AAVYAGRDREVMETGRPIVDRIYGFVADRSTDLNCVSVRPVIGTDG"
     gene            341..1444
                     /gene="mqnE"
                     /locus_tag="PLQ54_18330"
     CDS             341..1444
                     /gene="mqnE"
                     /locus_tag="PLQ54_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HOS95274.1"
                     /translation="MTGAPTVVPDPGLADIEARVLDGGSVTRDEIIRLYESDDLFAIG
                     RMANHVRERLNGNRAYYIINRHINPTNVCINRCRFCAFSRSANAPDAYTLSLDEIRQR
                     AEACQGTGVTEIHVVGGLHPDLPFDYYVDLLRTIRAALPEVHIQAFTAVEVAYLSRLA
                     GMPVRDTLRSLREAGLDSLPGGGAEVFAERVRAELCPDKLPGAAWLDVMRTAHELGIR
                     SNATLLYGHIETAAERADHLLALRELQDETGGFLSFIPLAFHAAHTDMPVWAPTSALD
                     DLKALAVGRIALDNFAHIKAFWIMLTPKLAQVALSFGVDDIDGTVVEEKITHAAGADT
                     PQALTRADLEGLIREAGREPVERDTLYNEVRAR"
     gene            1441..>1670
                     /locus_tag="PLQ54_18335"
     CDS             1441..>1670
                     /locus_tag="PLQ54_18335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95275.1"
                     /translation="MRYRVGVMEYLLGRPLVYGLQSLPEPVIDVQTDVPSRIGDRVLA
                     GDLDAGLMPAVQILENDGLWVLPGPCVACDGPV"
BASE COUNT          271 a          571 c          544 g          284 t
ORIGIN      
        1 tcccgtccgt gccgatgaca ggacgcaccg acacgcagtt gaggtccgtc gagcggtctg
       61 cgacaaaccc gtagatgcgg tcgacgatcg gccgcccggt ttccatcacc tcccggtcgc
      121 gtcccgcgta caccgccgcc tggtccggcg gatatagctc ttccgaggtg tagccgagca
      181 tgtcgtcaag cgagcgccag ccggagacgc atacgttgta gcggttggag tgcatgatgc
      241 gtccctcggc gttcttcacc gtgaacggca ccgtgagcct cgtgttcctg gcagccaccg
      301 cggtggatct ctacgtgctc gggagggcgg tgccgcggcc atgacggggg ccccaacggt
      361 tgtgcccgat cctggtctgg cggacatcga ggccagagtg ctcgacgggg gcagtgtgac
      421 ccgtgacgag atcatccggc tgtacgaatc ggacgacctg tttgccatcg ggcgcatggc
      481 caaccatgtg cgggagcgcc tgaacggcaa ccgcgcctac tacatcatca atcggcacat
      541 caaccccacc aatgtctgca tcaaccgatg ccggttctgt gccttcagcc ggagcgccaa
      601 cgccccggac gcatacacac tcagcctcga cgagatacgc cagcgggccg aggcgtgcca
      661 gggtaccggc gtgacggaga tccatgtcgt cggcgggctc catccggatc tgccattcga
      721 ctactacgtg gatctgctgc ggaccattcg agcggccctg cccgaggttc acatccaggc
      781 gttcaccgcc gtcgaagtcg cctacctgtc gcgtctcgcc ggcatgccgg ttcgggacac
      841 gcttcggtca ctccgcgagg cggggctgga ctcgcttccg ggaggcggtg ccgaggtgtt
      901 cgccgagcga gtgcgcgcgg agctctgccc cgacaaactg cccggagccg cgtggctgga
      961 tgtcatgcgg acggcccatg agctgggcat ccgctcgaac gcaaccctgc tctatggcca
     1021 catcgagaca gcggccgagc gggccgacca tctgctggcg ctccgcgagc tccaggacga
     1081 gaccggcggc ttcctgtcgt tcatcccgct ggcgttccac gcggcccaca ccgacatgcc
     1141 ggtgtgggca cccaccagtg ccctcgacga cctcaaggcg ctggccgtcg ggcggatcgc
     1201 gctggacaac ttcgcacaca tcaaggcctt ctggatcatg ttgactccca agctggccca
     1261 ggtcgccctc agcttcggcg tcgatgacat cgatggcacc gtggtggagg agaagatcac
     1321 ccacgccgcg ggtgccgaca ctccccaggc cctgacgcgc gcggacctcg agggtctcat
     1381 ccgcgaggcg gggcgcgaac cggtggagcg cgacacgctc tacaacgagg tgagggcccg
     1441 gtgaggtacc gcgtgggtgt catggagtac ctgctcggcc gtccgttggt ctacggcctg
     1501 cagtctctgc ccgagccggt gatcgatgta caaaccgatg taccctcgcg catcggagac
     1561 cgtgtcctcg ccggcgacct ggacgccggg ctcatgccgg ccgtccagat cctcgagaac
     1621 gatggtctat gggtactacc cggtccctgc gtggcctgcg acggcccggt
//
LOCUS       DAOSPL010001213         1668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_72944_length_1668_cov_2.949163, whole genome shotgun sequence.
ACCESSION   DAOSPL010001213 DAOSPL010000000
VERSION     DAOSPL010001213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72944_length_1668_cov_2.949163"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="PLQ54_18340"
     CDS             complement(<1..899)
                     /locus_tag="PLQ54_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95276.1"
                     /translation="MSAMLSGMHAEGAWLSYLGATRSCLSYLGSPATRAWLSGASGYA
                     FLIQMMPDGDSAGPIGFTGLCVHGCTGEVASGSVVRLLQNAGCASEAFCHCPFGGREG
                     TMDWARDEAWRLVRCSVDRGLPCIGFDVDGVDFAPICGYDDQGYYVLRAEPGTTTSVP
                     SGPVPWQSVGATIGWVRFESVARCEPAPDDVAVRHGLQAGYHRATHGKPMDGGAMGPA
                     AFDLWAQSLESGTIGSPGHRYCTAVYSELRRDAVAFLREARGRLPGRADALFAEALGH
                     YELVSERLNDVHALHPFVDGAETT"
     gene            complement(1421..>1668)
                     /locus_tag="PLQ54_18345"
     CDS             complement(1421..>1668)
                     /locus_tag="PLQ54_18345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOS95277.1"
                     /translation="RRGDMKLIEFFEDSHVELYDLAEDVGETRDLARERPDLAEELRQ
                     QLAEWRVSVGAQMPMVNPSYDPRRAHEWESYPPPWWE"
BASE COUNT          291 a          576 c          563 g          238 t
ORIGIN      
        1 gtggtctccg caccgtcgac gaacgggtgg agggcgtgta cgtcattcag acgctccgaa
       61 accaactcgt agtggcccag ggcctcagcg aacagcgcat cggcgcggcc gggcaggcgt
      121 cctcgtgcct cgcggaggaa agccaccgcg tcgcggcgga gctccgagta gaccgcggtg
      181 cagtaccggt gtcccggtga gccgatggtg cccgactcga gcgactgggc ccacaggtcg
      241 aacgcggccg gccccatggc tccgccatcc atgggcttgc catgcgtggc acggtgatac
      301 ccggcctgaa gcccgtgacg gacggccacg tcgtcgggcg ccggctcgca gcgggcgacg
      361 ctctcgaatc ggacccagcc gatggtcgcg ccgactgact gccatggcac ggggcccgac
      421 ggaacgctcg tcgttgttcc cggctcagca cgcagcacgt agtagccctg gtcgtcatac
      481 ccacagatgg gcgcgaaatc gacgccgtcc acatcgaagc cgatgcacgg gagaccgcga
      541 tcgaccgaac accgaacgag tcgccatgcc tcgtctcgag cccagtccat ggtgccctcg
      601 cgtccgccga acgggcagtg gcagaaggcc tcactggcgc aacccgcgtt ctggaggagg
      661 cgaaccacgg aacccgacgc gacctcgcct gtgcagccgt ggacacacag gccggtgaag
      721 ccgatggggc ccgcggaatc cccgtcgggc atcatctgga ttaagaaggc atagccgctg
      781 gcccccgaga gccacgctcg ggtggccggc gaacccagat acgacaggca gctccgcgtc
      841 gctcccaggt acgacagcca ggcgccctcg gcgtgcattc cactgagcat cgctgacatg
      901 acagcctcca tcccgatcgt ggcgggaacc ttacccgggc gccgggggga tgagcacgcg
      961 cagcccggca gacaacgcca gtggcacgcc cgcatgtggc gcagtatact cgaccataga
     1021 gggctcccca gttgggagct agcccggccc ggcaagccga ggtggcgggg ctgccgccaa
     1081 tctgatgccc cggtggccgc ggggtcctcg gcatcgggtt aagcgagacc gcgggtagtg
     1141 ctgccgacca gctcctggcc agagcgccgg agaacagaga gcacctcagg gtgccatagg
     1201 cagaccgcgg acgcgtccag ccgggttcgg gtgcgggcca gaaggtgatg cgtggagcgt
     1261 gactgacacc ttcaactggc gtgaggaccc gtcgtagttc ccctaccggc cgtcgaagag
     1321 ggctcggagc ccgtggagat cgtctccacc tgagccggaa cgtgggaacg ctccagacgg
     1381 gtcctccctg ttgtacggac ggctggaggc gactccagca ctactcccac cacggcggcg
     1441 ggtaggactc ccactcgtgc gcccgccgcg ggtcgtagct cgggttcacc atcggcattt
     1501 gcgccccgac cgacacccgc cactcagcaa gttgttgccg cagctcctcg gcgagatccg
     1561 gtcgttcccg agcgaggtcg cgggtctcgc cgacgtcctc ggccaggtca tagagctcca
     1621 catggctgtc ctcgaagaac tcgatgagct tcatgtcgcc gcggcgta
//
LOCUS       DAOSPL010001214         1668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_72970_length_1668_cov_2.259144, whole genome shotgun sequence.
ACCESSION   DAOSPL010001214 DAOSPL010000000
VERSION     DAOSPL010001214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72970_length_1668_cov_2.259144"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="PLQ54_18350"
     CDS             complement(<1..1254)
                     /locus_tag="PLQ54_18350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95278.1"
                     /translation="MIIGLVSALALGAVTPTNDLALGNTFIAGNHFPLGAFIVLFALA
                     VPVNLLLRRAGKPAPLCAGELITVWSMIVVSSGIPSSGLMRLLVPVPAGFHYFATPDN
                     EWAQYVAPHMPPWLVIEEGDASEWFFEGAPLGAGIPWRAWRAPTMAYGVFVLLFYLAT
                     VSAAALIRRQWETNERFTFPLCEMPLELAASAEGPVAGHDFLRHGGFWATVVVLIALH
                     SLNGLQRFRPSLPQFPFHFELGPYLTEPPWRYFGWVVLDTYPMMVGIAFFARNEVTFS
                     IWFFYLLRRVQEAVASFLGIPPGTAVFSWGPNYLLYQQMGGYIGLLCWCVWIARGHIR
                     VVVRKVWRGDGVDDGGEMLPYRLAFAGLLVGMLGCIAWLKVAGATVTAAVVAIVGLMG
                     SCVVMSWLITNGGTIMVQNRFSPSDL"
     gene            1474..>1668
                     /locus_tag="PLQ54_18355"
     CDS             1474..>1668
                     /locus_tag="PLQ54_18355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HOS95279.1"
                     /translation="MQQSRDFMKQLIETPSVSGFEEPAQRVVARYAHEFGASVQADVS
                     GNVLASIPGGNQPRVMLTGHV"
BASE COUNT          352 a          594 c          507 g          215 t
ORIGIN      
        1 caggtcggac ggcgagaacc ggttctgcac catgatggtc ccaccgttgg tgatgagcca
       61 gctcataacg acgcacgagc ccatcagccc cacgatggcc accaccgccg ccgtcacagt
      121 cgcacccgct actttcagcc acgcaatgca gcccagcatg cccaccagca gaccggcaaa
      181 cgcgagtcgg tatgggagca tctcgccgcc atcgtcgaca ccatcgcccc gccagacctt
      241 gcggaccacg acccggatgt gccctcgcgc gatccacaca caccagcaca gcaggccgat
      301 gtagccgccc atctgctgat agagcaagta gttcggcccc cagctgaaca cggcggttcc
      361 cggtgggatg cccaggaaac tcgcgaccgc ctcctgcaca cgccgcagca agtagaagaa
      421 ccagatcgag aacgtcacct cgttgcgcgc gaaaaaggca ataccgacca tcatggggta
      481 cgtgtccaga accacccagc cgaagtacct ccacggcggc tccgtgaggt agggccccaa
      541 ctcgaagtgg aacggaaact ggggtaggct cgggcgaaaa cgctgcaggc cgttgagcga
      601 gtgcagcgcg atcagcacca ccaccgtagc ccagaagccc ccgtgccgca ggaagtcgtg
      661 gccggcaact ggcccctcgg cgctcgccgc cagctcgagc ggcatctcac acaagggaaa
      721 ggtgaaccgt tcgttcgtct cccactgccg acggatcagc gccgccgccg acaccgtggc
      781 caggtagaac agcagcacaa acacaccgta cgccatcgtt ggagcacgcc acgcccgcca
      841 cgggatcccc gcccccagcg gggcgccctc gaagaaccac tccgacgcgt cgccctcctc
      901 gatgaccaac cagggcggca tatgcggcgc cacatactgg gcccactcgt tgtcgggtgt
      961 ggcgaagtaa tggaaacccg ccgggacggg gaccagcagc cgcatcagac cggacgaggg
     1021 aatgcccgac gaaacgacga tcatgctcca gaccgtgatc agctcgcccg cacagagcgg
     1081 cgccggcttt cccgcacgcc ggagaagaag gttcaccggc accgccagcg caaatagaac
     1141 gatgaacgcg cccagtggga agtgattccc ggcaatgaac gtgttgccga gagccaggtc
     1201 attcgtcggg gtcaccgcac ccagcgccaa agcactgacg agaccgatga tcactgagcg
     1261 taacgtgacg gggcgaggtc cgggagctcc gcggcggccc tgagtcgccg gcggatcgtc
     1321 acctggagct cgtgtaccgc gcatcgcaga caagggcagt ctccgccggc gtgatctccc
     1381 gggcaacgca tccagcccgg atgcttcccc gcctccggcg ccggcacctg cctgacgcgg
     1441 ccccgagggg ccacccggag ggagctagtg gtggtgcagc agtcacgcga cttcatgaag
     1501 cagctcatcg aaaccccatc ggtcagcgga ttcgaggaac ccgcccaacg cgtcgtggcc
     1561 cgctacgcgc atgagtttgg cgccagcgtc caagccgatg tcagcggcaa cgtgctggcc
     1621 tcgatcccgg ggggcaacca gccgcgggtt atgctcaccg ggcacgtc
//
LOCUS       DAOSPL010001215         1667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_73003_length_1667_cov_3.332506, whole genome shotgun sequence.
ACCESSION   DAOSPL010001215 DAOSPL010000000
VERSION     DAOSPL010001215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73003_length_1667_cov_3.332506"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..984)
                     /gene="larE"
                     /locus_tag="PLQ54_18360"
     CDS             complement(142..984)
                     /gene="larE"
                     /locus_tag="PLQ54_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOS95280.1"
                     /translation="MDRELQRKYARLQDILRDMGGVLVAFSGGVDSTLLLRVAHDVLG
                     PRALAVTACSETYPARERAEAREYIRAIGARAAEIETSELALPGFAGNPPDRCYHCKT
                     ELFSTLRRMAEAEGLPCVADGSNVDDLEDFRPGARALGELGIRSPLREAGLGKREIRE
                     LSRWLGLPTWNRPSAACLSSRFPYGTPITREALAQVEAAEDLLRSMGFRQIRVRHHSG
                     GLARIEVDPEEIHRFADPGLRQEVVASFRGIGYTYVSLDLQGYRTGSMNEVLPAHQRE
                     AAAV"
     gene            complement(1155..1364)
                     /locus_tag="PLQ54_18365"
     CDS             complement(1155..1364)
                     /locus_tag="PLQ54_18365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95281.1"
                     /translation="MHNAQHMYGWGYNPAWPCQPRIRHNGGMNIGFCDGHGKWMKYSQ
                     ITPDKLSAVKSGAWSGIQIPDIDYR"
     gene            complement(1388..>1667)
                     /locus_tag="PLQ54_18370"
     CDS             complement(1388..>1667)
                     /locus_tag="PLQ54_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95282.1"
                     /translation="LIYPYTKNMQISVCNSHYYDYTANNSWTSPLNLGRDGQPIGPGG
                     AFQISYCLNGIETYNGAWGRCHGLTARRGTSDRPDSPTPPSRRRPRHC"
BASE COUNT          263 a          544 c          586 g          274 t
ORIGIN      
        1 ggctgcatcg agcggggccg tatggtgctg ggcagcccgg ccacgccggt gccgacgacg
       61 agcacgtcac acgtgtgccg gtcgagcgtg gagagatcgc aatcgacgag ataccgccgt
      121 cccattgggt gccattcgcc tctagaccgc cgccgcctcg cgctgatggg ctgggagcac
      181 ctcgttcatg ctcccggtgc ggtagccctg gagatccagg gatacgtagg tatagccgat
      241 tccccggaag ctggccacaa cctcctggcg caacccggga tcggcaaagc gatggatctc
      301 ctcggggtcc acctcgatac gggccagccc ccccgagtgg tgccggacac ggatctgccg
      361 gaaccccatg ctgcggagaa ggtcctcggc cgcctcgacc tgggccagtg cctcgcgcgt
      421 gatgggggtg ccgtagggga atctggagga caagcacgca gccgacgggc ggttccaggt
      481 cggcaacccc agccagcgcg agagctcgcg gatctcgcgc ttgccgagtc cggcctcgcg
      541 gagcgggctg cgaataccga gttcgccgag cgcccgtgcg ccggggcgga agtcctccag
      601 gtcgtcgaca ttggagccgt ccgcgacgca cggcaagccc tcggcctcgg ccatgcgtct
      661 gagagtcgag aacagctcgg tcttgcagtg gtagcagcgg tcgggagggt tcccggcgaa
      721 gccggggaga gcgagttcgc tggtttcgat ctcggccgcg cgcgcgccga tcgcacggat
      781 gtactcgcgg gcctcggcac gttcgcgagc cgggtacgtt tccgagcagg ccgtgacggc
      841 cagagcgcgc gggcccagca catcgtgcgc cacccgcagc aggagcgtgc tgtccacgcc
      901 acccgagaag gcgaccagga cgccgcccat gtcgcgcagg atgtcctgta ggcgcgcgta
      961 ctttcgttgg agctcccggt ccacgggatt ccaccccgat tgcgactgcg cggccgccgg
     1021 gccgacgcga tcggcaacgc taccctccta agcataacgc ctcggggggc cgatggggtg
     1081 ccgaaccgct acagacaacg cgggcgcgct gagaacccgg cgcgcccgcg ttccgacgct
     1141 ccctgttcgg gcggctagcg atagtcgata tcgggaatct ggatcccgct ccaggccccg
     1201 cttttcacgg ccgagagctt gtcgggggtg atctgcgagt acttcatcca cttgccatgg
     1261 ccgtcacaga agccgatgtt catgccgccg ttgtggcgga tgcgcggctg acagggccag
     1321 gccgggttgt agccccagcc atacatgtgc tgagcattgt gcatgtcggg gcagcaggcc
     1381 tcgaagatca gcagtgtctc ggccggcgcc tcgatggcgg cgtcgggctg tccggccggt
     1441 ccgaagtgcc gcgacgcgcc gtcagaccat gacaccggcc ccacgccccg ttgtaggtct
     1501 cgatcccgtt gaggcagtag gagatctgaa atgcgccccc ggggccgatg ggttggccgt
     1561 cgcggcccag gttcaacgga gacgtccagg agttgttggc cgtgtagtcg tagtagtggc
     1621 tgttgcacac gctgatctgc atgttcttgg tgtacgggta gatgagg
//
LOCUS       DAOSPL010001216         1666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_73081_length_1666_cov_3.072005, whole genome shotgun sequence.
ACCESSION   DAOSPL010001216 DAOSPL010000000
VERSION     DAOSPL010001216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73081_length_1666_cov_3.072005"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PLQ54_18375"
     CDS             complement(<1..816)
                     /locus_tag="PLQ54_18375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95283.1"
                     /translation="MRAWCLLSSLSMALVGGHATAADSPASEGLITYCSGPFAYLISP
                     DGTNKVQLAHARMALQDVALAPDGVTMAWVRGTAFISHVYVQVDAATTMAVDVIRGRN
                     QRVGCLGFRPDSSALLYSGGGRVVLVDLPSCRTRVVARGDRGQWVAGGPQFIYETGAR
                     EEDEEIGLHLASVEGGPDELVVPYGCQAVVSADGARIAYRMPFASDDPRVYIMELASR
                     QSREMDQSTRFDRPLAFSPDGGLLLVDRADLSGDALCFQLCVLDVATGGIARLH"
     gene            986..>1666
                     /locus_tag="PLQ54_18380"
     CDS             986..>1666
                     /locus_tag="PLQ54_18380"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS95284.1"
                     /translation="MNDSDHGGMSRRTFLRTATVAAAAAGAFGLTRRGDAQDERPLRA
                     GLVGCGGRGTGAAHDFLNSSKNVTIVGLADAFQNRLDGARQSLKDDKGIDIADDHCFV
                     GYDAYEKLIGHEDVDIVLLATPPFFRPAHFACAVDAGKHVFMEKPMAVDPVGCREVMD
                     AAARADQKGLSVVVGTQRRHQKGYLECLQHVRDGAIGDIIGARAYWVGSPLGGWDRTD
                     HPTDLDEQV"
BASE COUNT          288 a          597 c          542 g          239 t
ORIGIN      
        1 gtgcagccga gcaatccctc ccgtggccac gtcgagcaca cacagctgga agcagagggc
       61 gtcaccgctc aggtcggccc ggtccaccag cagcaggcca ccatcgggcg agaacgccag
      121 gggacgatcg aaccgggtcg attggtccat ctcccgcgac tggcgggaag ccagctccat
      181 gatgtacacc cgtgggtcat cgctggcgaa gggcatgcgg taggcgatac gggccccgtc
      241 tgccgatacc acagcctggc aaccataggg caccacgagc tcgtcgggtc cgccctcgac
      301 gctcgcaagg tgcaggccga tctcctcatc ctcttcgcgg gccccggtct catagatgaa
      361 ctggggccca cccgcgaccc actggccgcg gtcgccccgg gcgaccaccc gggtccggca
      421 cgacggcaga tctaccagaa ccacccgccc cccacccgag tagagcaggg ccgacgaatc
      481 ggggcggaag ccaaggcagc ccacccgttg gttccgcccg cggatgacat ccacggccat
      541 ggttgtcgcg gcatccacct gcacgtacac gtggctgatg aacgctgttc cgcggaccca
      601 cgccatggtc acgccgtcgg gggccaaagc cacgtcctgc agggccatcc gcgcgtgcgc
      661 aagctgcacc ttgttcgtgc catcgggaga gatcagatag gcgaacgggc ccgagcagta
      721 ggtgatcagc ccctcgctcg ccggcgagtc cgcggccgtg gcgtggccac ccaccagtgc
      781 catgctcagg cttgagagga gacaccaggc acgcatgata cgctgattct cgcacgcccc
      841 caggggtgcg tcaacggcgt cacgcgcccg tgggcgctag ggttgacgct cggccggacc
      901 gtgggtacgc ttccggcggc ccacaggcga tggacggccc cgcacgggcg ggcggccgcc
      961 atccagctcc cgaggaggtt ccaccatgaa tgattcggat cacggcggga tgtcccgtcg
     1021 cacgttccta agaaccgcta ctgttgcggc ggccgctgcc ggggctttcg gcctcacgcg
     1081 ccgcggcgac gcgcaggacg agcgccccct gcgggccgga ctcgtcgggt gcggcgggcg
     1141 cggcaccggg gcggcccacg acttcctcaa ctcgtccaag aacgtgacca tcgtcggcct
     1201 ggccgacgcg ttccagaatc ggctcgacgg cgctcgccag agcctcaagg acgacaaagg
     1261 catcgacatc gcggacgacc actgcttcgt cggctacgat gcgtatgaga agctcatcgg
     1321 ccacgaggat gtcgatatcg tcctcctggc tacacctccg ttcttccgcc ccgcccactt
     1381 cgcgtgtgcc gtcgacgccg gcaagcacgt gttcatggag aagcccatgg ccgtcgaccc
     1441 cgtcggttgc cgcgaggtca tggatgccgc cgccagagcc gatcagaaag gcctgtcggt
     1501 cgtcgttggc acccaacggc gccaccagaa agggtacctg gagtgcctcc agcacgtccg
     1561 cgatggcgcc atcggcgaca tcatcggcgc ccgggcttac tgggtcggaa gcccgcttgg
     1621 gggatgggat cgcacggacc accccaccga cctcgacgag caggtt
//
LOCUS       DAOSPL010001217         1665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_73131_length_1665_cov_3.204969, whole genome shotgun sequence.
ACCESSION   DAOSPL010001217 DAOSPL010000000
VERSION     DAOSPL010001217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73131_length_1665_cov_3.204969"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1665
                     /locus_tag="PLQ54_18385"
     CDS             <1..>1665
                     /locus_tag="PLQ54_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95285.1"
                     /translation="AHLSQARACVRGLTDDLGDKATAGAMSAVEWAATERAALPGSDP
                     DAAARAYHATRGVSAVLNQWARIVSTGMAVDDLASTYGAAYVGAGGYAARARALSVTA
                     QALLAARPVDLAGAAGLAASIDELQRVALVRDNPLLSSSRLLFLRRRPGGGFPGLPQN
                     WQGNSSMGRTGWDTSINTLFPVRPDGEVTTLFHSDSRFVGDLCLSFDASHLLFSMPAE
                     EKGDTYQVWEIGADGGGLRMVTAASDNDYDNYDACYLPNGEIMFCSTRCYQAVPCTGG
                     DHVGLIYVMDADGSHVRQLTFDQDHSWNPTVMNDGRVVYTRWEYNDTPHYFSRMLFGM
                     NPDGTGQAALYGSGSYFPNTMMYATPIPGADSRLVCIISGHHGNPRGGEVAILDLARG
                     DREAAGVAQILPERHREVEPVIVDQYATGKWPQFVQPHPLSERYFVVSCKPSPDEPWG
                     IYLVDVFDNLVPILVDPEAALMEPIILAPRAVPPVIPKAVNYAKQDALVYLYDVYRGG
                     GLDGVPRGTIKSLRLVEPVYRYWGNGETYSASIDGTWDVKRILGTVP"
BASE COUNT          263 a          577 c          546 g          279 t
ORIGIN      
        1 cgcccatcta tcgcaggcca gggcgtgcgt tcgagggctg acagacgacc tcggcgacaa
       61 ggccacggcc ggcgccatga gtgccgtcga gtgggcggcg acggagcgcg ctgctctgcc
      121 gggctccgac ccggatgccg ctgcccgcgc gtaccacgcc acgcgcggcg tgagtgctgt
      181 gctgaaccaa tgggccagga tcgtcagcac cggcatggcc gtcgatgacc tggccagcac
      241 ctacggcgcc gcctacgtcg gtgctggcgg ctacgccgcg cgcgcccgtg ccctgtcggt
      301 caccgcccag gcgctgctag ctgcccgtcc cgtcgacctg gctggcgccg cgggcttggc
      361 ggcgagcatc gacgagctgc agcgagtcgc gctggtgcgg gataacccgc tgctctcgtc
      421 ctcccggctg ctgttcctca ggcgccggcc gggaggcggc tttcccggtc ttcctcagaa
      481 ctggcagggc aactccagca tgggccgcac cgggtgggac acctcgatca acacgctgtt
      541 cccggtacgc cccgatggag aggtcactac cctcttccac tccgactcgc gcttcgtggg
      601 cgacctgtgc ctctcgttcg atgcctcgca tctcctgttc tcgatgcccg cggaggagaa
      661 gggcgacacc taccaggtgt gggagatcgg cgcggatggc ggcggcctgc ggatggtcac
      721 ggctgccagt gacaacgact acgacaacta cgacgcctgc tacctgccga acggcgagat
      781 catgttctgc tcgacccgct gctaccaggc cgtgccctgc accggcggcg atcacgttgg
      841 cctgatctac gtcatggacg ccgacggcag ccacgtgcgg cagctcacgt tcgaccagga
      901 ccacagctgg aatccgaccg tgatgaacga cggccgcgtc gtctacacgc gctgggagta
      961 taacgacacg ccgcactact tcagccggat gctgttcgga atgaaccccg acggcaccgg
     1021 gcaggcggca ctctacggct ccggctccta cttcccgaac acgatgatgt acgcgacgcc
     1081 gatccccggg gctgactcga gactggtctg catcatatcg gggcaccatg gcaacccacg
     1141 aggcggcgag gttgccatcc ttgatctcgc ccggggcgac cgcgaggccg cgggtgtggc
     1201 gcagatcctg cccgagcgcc accgcgaggt cgagcccgtc atcgtcgacc agtacgccac
     1261 cggcaagtgg ccgcagttcg ttcagccaca cccgctctcg gagcgctact tcgtcgtgtc
     1321 gtgcaaacct tcacccgatg agccctgggg catctacctg gtcgatgttt tcgacaacct
     1381 ggttccgatc ctcgtggacc cggaggccgc gttgatggag ccgatcatcc tggcgccgcg
     1441 cgcggtgcca ccggtgatcc cgaaggcagt gaactacgcc aagcaggatg cgctggtcta
     1501 cctgtatgac gtgtaccgcg gcggcgggct ggatggtgtg ccccgaggga ccatcaagtc
     1561 cctccggctc gtggagcccg tgtaccgcta ttgggggaat ggcgagacct acagcgcctc
     1621 gatcgatggc acttgggatg tgaagcgcat cctggggacc gtgcc
//
LOCUS       DAOSPL010001218         1664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_73192_length_1664_cov_3.361715, whole genome shotgun sequence.
ACCESSION   DAOSPL010001218 DAOSPL010000000
VERSION     DAOSPL010001218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73192_length_1664_cov_3.361715"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1664
                     /locus_tag="PLQ54_18390"
     CDS             <1..>1664
                     /locus_tag="PLQ54_18390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95286.1"
                     /translation="AETASDVEQRTSYGWLASEPYRCFAVRVRILPDATVIQVSETVD
                     FGTVGHDQSLTNGEPYGYAPHRKVGLRTDGNLPLGLIPDPSGGGGQLGVGFVTTHLAS
                     DSSELGVTMPAERVEPDYVTPQLWSLMASRAGESVPSSRVFIDNPDLAHFPSRFAYDP
                     GEEAGNVRLYIGPMTPLGTYAGNGWAFVNTIDSGDPGVYDPPSADPNHPVPYDWLSVA
                     SSVLKVRVSEAPLPPRFGFGNLPYDPYIPDPPDPADPGTPADQNQVDRANIGNLDTNW
                     VETDPAVIVHLATDGRGTMSNLPIPSFTRDDLQIEMVFSSNRPRPGSSDPGYPRNWNL
                     WHAQMAPPAAPDPFHPVFVPEAPTLYAEDPTAEGVNPPEPAFPNLESWRHYGASFDQT
                     GEVLAWVLSSEWEGGGQVAVQQHIGVASRAGDFDDKVYFRIDAHRPEGVRPFVADVGD
                     PLVDGLYLFYYAGSGNNRRVYYAKVVDTEGTADDLSDDKLVGPVELRLMRQNCPRYEP
                     SATGVTASGTEFSAEPWVFADRYLDNGETKPCVGILFAGRSLSKGD"
BASE COUNT          273 a          594 c          521 g          276 t
ORIGIN      
        1 gcgccgagac ggcctccgac gttgaacagc ggacctccta cggctggctg gcgtccgagc
       61 cctatcgctg ctttgccgtg cgcgtccgta tcctgccgga tgccaccgtc atacaggtca
      121 gcgagaccgt ggactttggc acggtcgggc acgaccagtc gctcaccaat ggcgagccct
      181 acgggtacgc cccgcaccgc aaggtcggcc tccgcaccga tggcaacctg ccgctgggac
      241 tgatcccaga tccctctggc gggggaggcc agttgggggt cggctttgtc acaacccacc
      301 tcgccagcga cagctctgag ctgggggtca ccatgccggc cgagcgggtg gaacccgact
      361 acgtgacccc gcagctctgg tcactcatgg cctcgcgggc cggcgagtcc gtcccctcaa
      421 gccgtgtgtt cattgacaac ccagatctcg cccacttccc ttcgcgcttc gcatacgatc
      481 caggcgagga agccggtaac gtccgcctct acatcggccc catgactccg ctgggcacct
      541 atgcgggcaa tggatgggcc ttcgtcaaca cgatcgactc gggcgatccg ggcgtctatg
      601 acccgccctc ggcggaccca aaccacccgg tgccctacga ttggctgtcg gtggcgtcat
      661 cggtgctgaa ggtgcgtgtc tccgaagcgc cgctgccgcc gcggttcggc ttcggcaacc
      721 tgccctacga tccgtacatt cctgaccctc cggacccggc cgacccgggt acgcccgccg
      781 accagaacca ggtcgaccga gcgaacatcg gcaacctgga cacgaactgg gtcgagaccg
      841 accccgccgt catcgtgcac ctggccactg atggccgggg gaccatgagc aatctgccca
      901 tcccgagctt cacccgcgac gacctgcaga tcgagatggt gttctcgagc aaccggccgc
      961 gtccgggctc cagcgatcca gggtacccaa ggaactggaa cctgtggcac gctcagatgg
     1021 ccccgcctgc cgcccctgat cccttccatc cggtcttcgt gcccgaggcg cccacgctct
     1081 acgccgagga ccccactgcg gagggcgtca acccgcccga gcccgctttc cccaacctgg
     1141 agtcctggcg gcactatggc gcctcgttcg accagaccgg tgaggtgctc gcctgggtgc
     1201 tgtcgtccga atgggaaggt ggggggcagg tcgcggttca gcagcacatt ggggtcgcga
     1261 gccgggcggg cgacttcgat gacaaagtct acttccgcat cgatgcgcac cggcccgagg
     1321 gcgtcaggcc cttcgtcgcc gacgtcggcg acccattggt cgatggcctc tacctgttct
     1381 actacgccgg gtccggcaac aaccggcgcg tctactacgc caaggtggtg gacaccgaag
     1441 ggacagccga tgacctgagt gacgacaagc tggtgggccc ggtcgagctg aggctcatgc
     1501 gccagaactg cccgcgctat gagccgtccg cgaccggagt cacggcttcc ggcaccgagt
     1561 tctcggccga gccctgggtg ttcgctgacc ggtatctcga caacggcgag accaagccgt
     1621 gtgtcggcat cctgttcgcc ggccggagtc tcagcaaggg cgac
//
LOCUS       DAOSPL010001219         1664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_73229_length_1664_cov_2.152890, whole genome shotgun sequence.
ACCESSION   DAOSPL010001219 DAOSPL010000000
VERSION     DAOSPL010001219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73229_length_1664_cov_2.152890"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PLQ54_18395"
     CDS             complement(<1..347)
                     /locus_tag="PLQ54_18395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95287.1"
                     /translation="MRDTTPKTPRNGGICRREFCLRTGQVVLGAPLLMGALSDRLFGA
                     VERQCEWKKGGLFGPGHKATATVRSAFLRPRGEYWLGWPGTSFDIEGHRKMWTEGIEQ
                     SGKVGNEPSVDDG"
     gene            511..1446
                     /locus_tag="PLQ54_18400"
     CDS             511..1446
                     /locus_tag="PLQ54_18400"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOS95288.1"
                     /translation="MTRREWLKRTGTALASASMVGGIGMAAVARPDFANLRLGVSISA
                     ANAEQAASEATWATKAGFGRVQLNVWGGALTDQQVAALCAPITGAGLKIVALGCYINP
                     LDPANLSYMGASVDLMRSLAGNHRAHGARTFVTWSGGYHDRFAGSDPRNASPEAFEAL
                     VEATGELLAVLEPCRGRIAFEPFFPDVLGTVDRFAEMFRRMPTSRVGLVMDPPNFVRP
                     EDYLRRSEIMGRAFERLGPRVLVAHLKDIGRNPDGSVTYPAPCEGEMNYAAYLTCLAS
                     LGRPVECILEHTGGEHMGHSRERVLRALDEAQARA"
BASE COUNT          256 a          579 c          575 g          254 t
ORIGIN      
        1 atgccgtcat ctacggacgg ctcgttgccg accttgccgg actgctcgat gccctcggtc
       61 cacatcttgc gatggccctc gatgtcgaag ctcgtgcccg gccagcccag ccagtactcg
      121 ccccgcgggc ggaggaaggc cgagcgcacc gtggccgtgg ccttgtggcc ggggccgaag
      181 aggccaccct tcttccactc acactgccgc tcgacggcgc cgaagagtcg gtcggagaga
      241 gcccccatca gcaggggagc gccgagaacc acttggccgg ttcgcaggca gaactcgcgg
      301 cggcagatgc caccgttccg gggggtcttc ggggtcgtgt ctcgcatgag tcgcgcgcct
      361 ccaccttggt cgggttcatg ggacagcagt caggcgacta gttccgcgcg ccccggggtc
      421 atccttcccc ctcccagccc cggaggttgc tgaacggcac ccgaggaacg ggaccgcatt
      481 gacggagact gacaacccgg aggcgcaccc atgacccgca gggagtggct caagagaacc
      541 ggaacggctc tggccagcgc gtcgatggtg ggaggcatcg gaatggccgc tgtggcgagg
      601 ccagacttcg ccaaccttcg gctcggcgtc tcgatctccg ccgccaacgc cgagcaggcc
      661 gcatcggagg ccacgtgggc caccaaggcc ggctttggcc gtgtccagct caacgtgtgg
      721 ggcggagcgc tgaccgacca gcaggtcgcg gccctctgcg cgcccatcac cggcgccggt
      781 ctcaagatcg tggccctcgg gtgctatatc aacccgctcg atcccgcgaa cctgtcctat
      841 atgggcgcct cggtggacct gatgcggtcg ctggccggga accaccgcgc ccatggcgcc
      901 cggacattcg tcacctggag cggcggctat cacgaccgct tcgccggtag cgatccgcgc
      961 aacgcctcgc ccgaggcgtt cgaggcactg gtggaggcca ccggcgagct gctggcggtc
     1021 ctcgagccct gccgcgggcg catcgccttc gagcccttct tcccggacgt cctcgggacc
     1081 gtcgaccgct ttgccgagat gttccgccgg atgcccacaa gccgtgtcgg gctcgtgatg
     1141 gatcccccga acttcgtgcg gcccgaggac tacctccgga ggagcgagat catgggcaga
     1201 gcctttgagc ggctcgggcc gcgagtgctc gtggcgcacc tgaaggacat cgggcgaaac
     1261 ccggacggct cggtaaccta cccggcgccg tgcgagggcg aaatgaacta cgcggcgtac
     1321 ctcacatgcc tggcgtcgct cggccggccc gtggagtgca tcctcgagca caccggcggc
     1381 gagcacatgg gccattcacg cgagagggtg ctccgcgcac tcgacgaggc gcaggcgcga
     1441 gcgtagcccg gtgccgctgc tgacccgacg tgcgatggtc cggtcggctc gctggatagc
     1501 ggtaggccgt gcctgcccac gccgaccgcc gggctggtgg tttcggtccg ctgcccgcac
     1561 tgagccttag gacggcagga ggcgatcgcg ggacgagcag gaggcttctg cgcgagtgac
     1621 tggccgatgc gaaactggcc ccggaggacc tcgaggtgac catc
//
LOCUS       DAOSPL010001220         1663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_73258_length_1663_cov_3.233209, whole genome shotgun sequence.
ACCESSION   DAOSPL010001220 DAOSPL010000000
VERSION     DAOSPL010001220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73258_length_1663_cov_3.233209"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1527)
                     /locus_tag="PLQ54_18405"
     CDS             complement(<1..1527)
                     /locus_tag="PLQ54_18405"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOS95289.1"
                     /translation="MPESNHPSGGFFGPEACGRNFRRLLEAHPPYVDPQSSLLGGYSV
                     NFLAYRTVGWPPELSLPHLAAEQAKYGLHTGIGAAQHFCQDLAIGLELGYGGLLAKIA
                     GCRAASGPEHEGFYAGLEHVVRGIQAWIATNVAEARRRAGLEGEMEARQSLLELAEMN
                     QRLVTAPPQTFREACQWMAWYDMAARAYNGSGSLGRLDVLLWPYYERDRAAGVLTDEE
                     AILHLASLLVMDTAYIQLGGYDVEGRDATNAVSYLVLEAAHRLRIPANVAVCVGRGID
                     PGLMRRGVEVLLADKAGVPKFLGTDNLIDGFARNGVPLEIARARAYSGCHWSALPGRE
                     YTLNDCVKVNLVAVFVAAWDEMLAGGQPSVGRLWGLFERHLRRAIEVLAEGLDFHMEH
                     MHGVMPELVIDLLCHGTIERGLDASHGGVDYVNLCVDGAGLATVADSFGALAQYVESS
                     RSLSWDEVIGHVEANWAGGAGERARLRMRHAPRYGSGGSAGDEFALRITRLFTSLVKE
                     R"
BASE COUNT          271 a          638 c          502 g          252 t
ORIGIN      
        1 tctctccttc accagactcg tgaacagccg cgtgatgcga agcgcgaact catcgcccgc
       61 cgagccgccc gagccgtagc gcggagcgtg gcgcatccgc agacgcgcac gctcgcccgc
      121 cccaccggcc cagttggcct cgacgtgccc gatgacctcg tcccagctca ggctccggct
      181 cgactcaaca tactgggcca gcgcgccgaa cgagtccgcg accgtcgcca ggccagctcc
      241 gtcgacgcac aggttcacat agtcgacccc gccatgcgac gcgtccagcc ctcgctcgat
      301 cgtgccgtgg cagaggagat cgatcacgag ctcgggcatc accccgtgca tgtgctccat
      361 gtggaagtcc aggccctcgg ccagcacctc gatggcccgc cgcagatgcc gctcgaacag
      421 gccccacagg cgcccgacgc tcggttgccc ccccgcgagc atctcatccc acgccgcgac
      481 gaacacggcc accaggttga ccttcacgca gtcgttcagc gtgtactcgc ggcccggcag
      541 ggcgctccag tggcagcccg agtacgctcg tgcccgggcg atctcgaggg ggaccccgtt
      601 gcgggcgaag ccgtcaatca ggttgtcggt gcccaggaac ttcggcacac ccgccttgtc
      661 cgcgaggagc acctcaacgc cccgccgcat cagcccgggg tcgatgccgc gccccacaca
      721 caccgcgacg ttcgccggga tccgcagccg gtgagcggcc tcgaggacca gatacgacac
      781 cgcgttggtc gcgtcacgcc cctcgacgtc gtacccacca agctggatgt acgcggtgtc
      841 catcaccagg agtgacgcca gatgcaaaat ggcctcttcg tccgtaagga ccccggctgc
      901 gcgatcgcgc tcgtagtagg gccacagcag cacatcgagc cgccccagcg agccgctgcc
      961 gttgtaggcc cgggccgcca tgtcatacca agccatccac tggcacgcct cacgaaaggt
     1021 ctgcggcggg gccgtcacga ggcgctggtt catctcggcg agctcgagca ggctctgtcg
     1081 cgcctccatc tccccctcca ggcctgccct cctgcgtgcc tcggcgacgt tcgtcgcgat
     1141 ccatgcctgg atcccccgaa ccacgtgctc gagcccggcg tagaacccct cgtgctccgg
     1201 cccgctggcc gcgcggcacc cagcgatctt cgccagtaga cccccgtacc ccaactccag
     1261 cccgatggcc agatcctggc agaaatgctg ggccgcgccg atgccggtgt gcaggccata
     1321 cttcgcctgt tccgcggcca agtgcgggag gctcagctcc ggcggccagc ccaccgttcg
     1381 gtacgccagg aagttcaccg agtacccgcc gagcaacgag ctctgtgggt ccacgtaagg
     1441 tgggtgcgcc tcgagcagac ggcggaagtt gcgtccgcag gcctccggtc caaaaaagcc
     1501 gccgctcggg tggttcgact ccggcacgaa ccccttcagg cgcacgtcgg tgatgggacc
     1561 ccggagccgc tgatggtctg taccgtctcc cgtcgatccg gcggcggcag gatcaacgcc
     1621 cagtcgtcgt gatccatcga cccgatgacc tgctgtttct ctc
//
LOCUS       DAOSPL010001221         1662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_73330_length_1662_cov_3.738021, whole genome shotgun sequence.
ACCESSION   DAOSPL010001221 DAOSPL010000000
VERSION     DAOSPL010001221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73330_length_1662_cov_3.738021"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PLQ54_18410"
     CDS             <1..182
                     /locus_tag="PLQ54_18410"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HOS95290.1"
                     /translation="SPCCPLFEVEVGPGRCGMVISAGLNPIAAVEEARIPTRSHAMAA
                     VEDFEHLTDINELSL"
     gene            complement(214..768)
                     /locus_tag="PLQ54_18415"
     CDS             complement(214..768)
                     /locus_tag="PLQ54_18415"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOS95291.1"
                     /translation="MTACFDPHRPELAWRGTNAYGFAIVHDSRLGMRIEFVPDPGLPD
                     GVQPFGVVAVPVFDHRLVVVQVRGQSGWEFPGGHVELGELPEQAVRRELMEEAAAEAD
                     WLAPIAHEVLTEDEGRRRYLLVYLARVSRLAPLGERGEVTGRALMPPSQALGRGLSGR
                     WGQQHIAQVLEFCVARARELGVPV"
     gene            complement(765..>1662)
                     /locus_tag="PLQ54_18420"
     CDS             complement(765..>1662)
                     /locus_tag="PLQ54_18420"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOS95292.1"
                     /translation="TIEERVTPRTRAILAVHIFGQPANMSAIRAIANRHGLVVIEDGS
                     QAHGALHQNQKVGTFGDASGFSCMGGKLLASSEAGYMVTPRADVYWKASLCTQHMGRS
                     SEPGFPEDLLRYVDSLVYTYRLSPINAVLLTAQLRKIDAENQGRRRNVAHFRQAMAGV
                     ESARFPSYAEGDDPVYHMLTMNFCPEVAGVSRDTYLAALHAEGVGIFAYVPSPIPTWP
                     RLNWQTYTGPRVMWTESLRQSGVDYRTMECPNAHTKVARSLEMSWNYIEEDPERIQRL
                     AAAFWKVEENLGALREWERRGA"
BASE COUNT          278 a          566 c          555 g          263 t
ORIGIN      
        1 gatcaccatg ctgcccgctc ttcgaggtcg aggttggccc gggccgctgc ggcatggtga
       61 tcagtgcggg gctgaacccg atcgctgcgg tcgaggaggc gaggatcccc acccggagcc
      121 acgccatggc tgcagttgag gacttcgagc acctgacgga catcaacgag ctcagcctgt
      181 aggttcgctg gattgccggg gcttcagcgg gctctatacg ggcacgccga gctctctggc
      241 ccgagccacg cagaactcga gcacctgggc gatgtgctgt tggccccatc ggcccgacag
      301 cccccggccg agcgcctgac tgggcggcat cagggcgcgc ccggtgactt cgccccgctc
      361 gccgagtggc gcgagccgag acacgcgagc gaggtacaca agcagatagc gccgccggcc
      421 ttcatcctcg gtaagcacct cgtgggcaat gggagccagc cagtcggcct cggcggccgc
      481 ttcttccatc agctcgcgcc gcacggcctg ctccggcaac tccccgagct cgacatggcc
      541 gcccgggaac tcccatcccg actgcccgcg cacctgcacg accaccagcc ggtgatcgaa
      601 gaccggtacg gcgacgacgc cgaagggctg cactccatcc ggcagccccg ggtcgggcac
      661 gaactcgatg cgcataccaa gccggctgtc atggacgatg gcgaaaccat atgcgttggt
      721 gccgcgccac gccaactccg gccgatgcgg gtcgaagcac gcggtcacgc gccgcggcgc
      781 tcccactcgc gcagcgcgcc gaggttctcc tcgaccttcc agaaggccgc ggccagccgc
      841 tggatcctct ctgggtcttc ctcgatgtag ttccagctca tctcgagcga gcgcgccact
      901 ttggtgtgcg cgttcggaca ctccatggtg cggtagtcga cgcccgactg acgaagcgac
      961 tcggtccaca tgaccctggg gccggtgtac gtctgccagt tgaggcgggg ccacgtcggg
     1021 atgggcgagg gcacataggc gaagatgccg acgccctcgg catgtagggc agccagatag
     1081 gtgtcgcggg acacgcccgc gacctcgggg cagaagttca tggtgagcat gtgatacacc
     1141 gggtcgtcgc cctcggcgta ggaggggaag cgcgccgact ccaccccggc catggcctgg
     1201 cggaaatggg ccacgttccg ccgtcggcct tggttctcgg cgtcgatctt gcgcagttgc
     1261 gccgtcagga gcacggcatt gatcggagag agccggtagg tatacacgag ggagtccacg
     1321 tagcgaagga gatcctcagg gaaaccgggc tccgacgagc gccccatgtg ctgcgtgcac
     1381 agcgatgcct tccaatagac atcagcccgg ggcgtgacca tatagcccgc ctcgctggat
     1441 gccagcaact tgccgcccat gcacgagaag ccactcgcat cgccgaatgt acccaccttc
     1501 tggttctggt gcagcgcccc atgcgcctgg ctgccgtcct cgatgaccac caggccgtgc
     1561 cggttggcga tggcgcggat ggcggacata ttcgccggct gaccgaagat gtggacggcc
     1621 aggatggccc gggtccgcgg cgtgactcgt tcctcgatgg tg
//
LOCUS       DAOSPL010001222         1660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_73451_length_1660_cov_3.760748, whole genome shotgun sequence.
ACCESSION   DAOSPL010001222 DAOSPL010000000
VERSION     DAOSPL010001222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73451_length_1660_cov_3.760748"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLQ54_18425"
     CDS             <1..227
                     /locus_tag="PLQ54_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95293.1"
                     /translation="AATPYIGERTIVAVYSGPGGVSEGIRDGLRAAGLSRQVLAEARL
                     PSAGWTRWFWLASATDLDGFPSGSTEDCFT"
     gene            392..1198
                     /locus_tag="PLQ54_18430"
     CDS             392..1198
                     /locus_tag="PLQ54_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOS95294.1"
                     /translation="MSRVLAVVNQKGGVGKTTTAVNLSAALADLGLSVLLVDMDAQGN
                     ATTALGIERHSLEQCIYSALVAQISDDDRGPTLAHVSLDTGIHGLKLVPARMDLAAAE
                     LELASALAREMVLRRLLAPVRESFDFVLLDSPPSLSLLTVNCLTAADGILIPIQCEYY
                     ALEGLSQLLRVVRMVESSLNPSLSLQGVVLTMYDGRTRLSRDVANEVRRHMGAKTYRT
                     PIPRNIRISEAPSHGLPVTRYDPESAGARAYRLLAKEVVQREEAWTGARA"
     gene            1167..>1660
                     /locus_tag="PLQ54_18435"
     CDS             1167..>1660
                     /locus_tag="PLQ54_18435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOS95295.1"
                     /translation="MKRPGLGRGLDALIPQVPPPGGRSVAEIDISTIQPNPYQPRQTV
                     DDDTIRELAESIRAHGVLQPIVVRQSENGYQLVAGERRWRAARAAGLATIPALVRQAN
                     DLQMLQVALVENLQREDINAMDAAAAYGQLMDEFGATQEEVAEAVGKSRTAVANTVRL
                     LKLPP"
BASE COUNT          285 a          615 c          502 g          258 t
ORIGIN      
        1 gtgcggcaac gccctatatc ggcgaacgaa cgatcgtcgc cgtgtactcg ggcccgggtg
       61 gagtgagcga ggggatccgg gacggtctcc gagctgcggg gttgtcgcgg caggtattgg
      121 ccgaggcgcg cctacccagt gcgggatgga ctcggtggtt ctggctggcc tcggccacag
      181 acctcgatgg gttcccgagt ggctccaccg aggattgttt cacgtgaaac acggtgctcg
      241 agtgcttgtc cgatgtccag accggccctg tttcacgtga aacaaccgcc ctacgctctc
      301 gcgagaccct gcccggctct cgccggccga ggggaatgac acgtaatggc ccgggcttgg
      361 cgcgcccggt ccaccatgga ggacacgccc cgtgagccgc gtcctggctg tggtgaacca
      421 gaagggcgga gtcggcaaga cgaccaccgc cgtgaacctc agcgccgcac tcgctgacct
      481 cggcctgagc gtgctcctcg tcgacatgga cgcccaaggc aatgccacca cggccctggg
      541 catcgagcgc cattcgctgg agcagtgcat ctactcggcc ctcgtcgccc agatctccga
      601 cgatgaccgt gggcccaccc tggcccacgt ctccctcgat accggcattc acggcctgaa
      661 gctcgttccc gcccgcatgg acctcgccgc cgccgagctc gaactcgcgt cggccctcgc
      721 ccgcgagatg gtcctccgca ggctcctggc ccccgtgcgc gagagcttcg acttcgtcct
      781 cctggactcg cctccaagcc tgagcctgct caccgtcaac tgcctcacgg cggccgacgg
      841 catcctcatc cccatccagt gcgagtacta tgcgctcgag ggactctcgc aactcctccg
      901 cgtcgtccgc atggtcgagt cgtccctcaa ccccagcctg agcctccaag gcgtcgtcct
      961 caccatgtac gacggccgaa cacggctgtc cagagacgtc gccaacgagg tccggcgcca
     1021 catgggcgcg aaaacctatc gcacgcccat cccgaggaac atccgcatca gtgaggcgcc
     1081 gagccacggc ttgcccgtca cccgatatga tcccgagagc gctggagctc gggcctacag
     1141 gctcctggcg aaagaggtgg tgcagcgtga agaggcctgg actggggcgc gggcttgacg
     1201 ccctcatccc gcaagttcct cctcccggcg gccggtcggt ggccgagatc gatatctcga
     1261 ccatccagcc caacccctac cagccccggc agaccgtcga cgacgacacg atccgtgaac
     1321 tagcggaatc catccgcgcc cacggagtgc tccagcccat cgtcgtgcgg cagagcgaga
     1381 acggatacca gctcgtggcc ggtgaacggc gctggcgagc cgcgcgtgcc gccggactcg
     1441 ccaccattcc cgctctcgtg cgccaggcca acgacctcca gatgctccaa gttgccctcg
     1501 tcgagaacct ccaacgcgag gacatcaacg ccatggacgc cgctgccgcc tatgggcagc
     1561 tcatggacga gttcggagcc acccaggagg aagtcgccga ggccgtcggc aagagccgaa
     1621 ccgccgtcgc caacaccgtc cggctcctca aactcccccc
//
LOCUS       DAOSPL010001223         1660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_73454_length_1660_cov_3.469782, whole genome shotgun sequence.
ACCESSION   DAOSPL010001223 DAOSPL010000000
VERSION     DAOSPL010001223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73454_length_1660_cov_3.469782"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1633)
                     /locus_tag="PLQ54_18440"
     CDS             complement(<1..1633)
                     /locus_tag="PLQ54_18440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95296.1"
                     /translation="MLQLLGASDQAYAVPRVQMDAPDWWLDAHEDELTRYASGVGWES
                     NGWGGTKHPSFASLVWRREAGEALRQLVEHILASPYSDRVIGIHVASGIYGEWHYWSA
                     TDFPDVSEPMRQKLIRDMRARYGTPEAWSRAWGQPVAPFETATIPSVEERQNGDVGMF
                     RDPGRSRKVMDYYDTLFGAQIDAIEDFCRVVKEASDDRLLTLVFCGYLPDLEWTQEGD
                     HRLFIRALRSPVIDAFSSPHSYHRRGLGADGYFRNFPASVRLHGKLFMDEGDDRTYRA
                     NDPTFTAVTNTEESLQLIRREFGNAIANDVGLWYMDQQGDWFHDDAIMDEIGLLKRWA
                     DRALRRPKQRHSEVGVFYSMATEPYLAGRTSGRNRVSAPMTNAQLGELCKAGAPFDFY
                     VMDDLTDDRLPEYRVYVMLDAQYLTPAQRATVVRLRNEGASFVWIHAPGFITEDGLSI
                     EAACEIAGMRLRMLTSGTCQVMAADGTTLGPGGYQAPLFAIDDPGAEVLGTLTGTELP
                     GLAVRAAGRSLSLFSSVPGVPARVLREFWARAGVHI"
BASE COUNT          274 a          555 c          559 g          272 t
ORIGIN      
        1 agatgtggac gccggcccgg gcccagaact cgcggagaac ccgcgcgggc acgccgggca
       61 ccgaagagaa cagcgacagc gagcgtcccg cggctcggac ggcgagtccg ggcagctcgg
      121 tcccggtgag cgtccccagc acctcggctc cggggtcgtc gatcgcgaac aggggtgcct
      181 ggtaaccgcc ggggccgagg gtcgtaccgt cggcggccat gacttggcat gtcccgctcg
      241 tcagcatgcg caggcgcatc cccgcgatct cgcacgccgc ttcgatcgag agcccatcct
      301 cggtgatgaa cccgggcgcg tggatccata cgaacgacgc accctcgttc cgcagacgca
      361 ccacggtggc tcgctgcgcc ggcgtgaggt attgggcgtc cagcatcacg tagaccctgt
      421 actcgggcag tctgtcgtcg gtcaggtcgt ccatcacata gaagtcaaat ggcgcgcctg
      481 ccttgcacaa ctccccgagc tgggcgttgg tcatcggagc cgacacccgg ttccggcccg
      541 aggtgcggcc ggcgaggtac ggctcggtgg ccatcgagta gaacacgccg acctcggagt
      601 gccgctgctt ggggcggcgg agtgcgcgat cggcccagcg cttcagcagg ccgatctcgt
      661 ccatgatcgc gtcgtcatgg aaccagtcgc cttgctggtc catataccag agacccacat
      721 cattggcgat ggcgttgccg aactcgcgcc ggatgagctg gaggctctcc tcggtattgg
      781 tgactgcggt gaatgtggga tcgttcgcgc ggtaggtccg gtcgtcgccc tcatccatga
      841 acagcttccc gtggaggcgc accgacgccg ggaagttgcg gaagtagccg tcggcgccca
      901 aaccccgccg gtgataggag tgcgggctgc tgaaagcgtc gatcaccggc gaccggagcg
      961 cgcggatgaa gagacggtgg tcgccctcct gggtccactc gagatcggga agatagccgc
     1021 agaagacgag ggtcagcaga cggtcgtcgc tcgcttcctt gacgacacgg cagaagtcct
     1081 cgatggcatc gatctgcgcg ccaaagagtg tgtcgtagta gtccatcacc ttccggctcc
     1141 tgcccgggtc tcggaacatg ccgacatcgc cattctgccg ctcctcgacg ctcgggatgg
     1201 tggccgtctc gaatggcgcc accggctggc cccaggcacg gctccatgcc tcgggcgtgc
     1261 cgtaacgcgc cctcatgtct cgaatgagct tctgccgcat gggctccgac acatcgggga
     1321 agtcggtcgc gctccagtag tgccactcac cgtagatgcc cgaggccacg tggatgccga
     1381 tcacgcggtc cgagtagggg ctcgcgagga tgtgctcgac aagctggcgc agcgcctcgc
     1441 ccgcctcccg ccgccagacc agtgaggcga acgaggggtg cttggtgcca ccccagccgt
     1501 tgctctccca accgacgcca ctggcatagc gcgtgagctc gtcctcgtgg gcgtcgagcc
     1561 accagtcggg ggcgtccatc tggacccggg gcacggcgta ggcctggtcg ctggccccga
     1621 gcaactgcag catcaaccgg tccacgtcgg agtggtcata
//
LOCUS       DAOSPL010001224         1660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_73466_length_1660_cov_2.947664, whole genome shotgun sequence.
ACCESSION   DAOSPL010001224 DAOSPL010000000
VERSION     DAOSPL010001224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73466_length_1660_cov_2.947664"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PLQ54_18445"
     CDS             <1..1137
                     /locus_tag="PLQ54_18445"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOS95297.1"
                     /translation="VGAAGPIDGDQATAQTIVHAFVDLDLPSLTGGKRAFINFTREML
                     VGGIAELLPPDAIVVEVLETVDADDQVVEACTRLKHAGYQLALDDYLLESEQHQRLLP
                     LADVVKVDFMGNDLRAREEAVRRLKSPGRLLLAEKVETEPEFEWARQHGYTLHQGYFF
                     ARPTSVQGQQIPPAKLNYLRLLNALRNRELDLDAVEAAVRDDVSLTHRLLRLLNSASF
                     SWRQRIGSVRHALVALGEDATRKWLSLLCTMGIATDRPAELVVLSLTRARFLEEVSGL
                     IGLEARSGDLFFMGMVSLLPAILAREAAEVYAQLALPDDVRQALMGGGNVLASALRMA
                     LVFERAEWSRLPSLCADLGTTPRAVSDAYIRAARHATRALGTED"
     gene            1303..>1660
                     /locus_tag="PLQ54_18450"
     CDS             1303..>1660
                     /locus_tag="PLQ54_18450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95298.1"
                     /translation="MDFWVGRQPIFDGQVVVQAYELLYRQGQQGLRSSDQGDQATAQT
                     IVHSLLDLGLPSLTGGRRAYINFTREMLLGGYVELLPKDGVVVEILETVDVDDAVVGV
                     CSELQRAGYCLALDDFT"
BASE COUNT          263 a          526 c          565 g          306 t
ORIGIN      
        1 gtgggcgctg ccggtcccat cgatggcgac caggcgacgg cccagacgat cgtccacgcg
       61 ttcgtggacc tcgacctacc gtcgctcacg ggcggaaagc gtgcgttcat taacttcacc
      121 cgcgagatgc tcgtcggagg catcgccgag ctactgccgc cggatgccat cgtggtcgag
      181 gtactcgaga ccgtggacgc cgacgaccag gttgtcgagg cctgcacccg gctgaagcac
      241 gcggggtacc agttggctct tgacgactac ctattggagt cggaacagca ccagcggctc
      301 ctgcccctgg ccgatgtggt gaaggtcgac ttcatgggta atgacctgag ggcccgggag
      361 gaggccgtgc ggcgcctcaa gagtccgggc cgcctcctgc tggccgagaa ggtggagacg
      421 gagccggagt tcgagtgggc ccgccaacac ggttacacgc tacaccaggg gtacttcttc
      481 gcgcggccga cctcggtcca ggggcagcag ataccgcctg ccaagctcaa ctacctgcgg
      541 ctgctgaatg ctttgcgcaa ccgcgaactg gacctcgatg ccgtcgaagc ggcagttcgg
      601 gatgatgtct cactcacaca caggcttctg aggctgctca actccgccag cttctcgtgg
      661 cgccagcgga tcggctcagt gcgccacgcg ctcgtggcgc tgggagagga tgccacacgc
      721 aagtggctct cgctcttgtg taccatggga atcgcgaccg atcggcccgc cgagcttgtg
      781 gtgctctcgc tgacgcgcgc ccggttcctc gaggaggtct cgggcctcat cggtctcgag
      841 gcccgtagtg gtgatctgtt cttcatgggc atggtatccc tgctccctgc gatcttggcg
      901 cgcgaagcag cggaggtcta tgcgcagctt gccctgcccg acgacgtgcg acaggcgctc
      961 atggggggcg gcaacgtcct ggcgagtgcc ctgcggatgg ccttggtgtt cgagcgagcc
     1021 gagtggtcgc ggcttccgtc attgtgcgcg gaccttggca ccactccccg cgcggtgtcg
     1081 gacgcttaca tccgggccgc tcgccacgcg acgcgcgccc tgggcactga ggactaagtc
     1141 tccagcccga gaccaagatg gcgaggcggt caccgtgtcg taagcatccg ggagggattg
     1201 cgtaccgatt ccctgaagac gggctctcag cggacgcgtc ggaagcctgg atgggcgagt
     1261 catgcggtta cgcccaagcc agtggattga ggggcggtcg cagtggactt ctgggtgggc
     1321 cggcagccca tcttcgacgg acaggtagtc gttcaggcct acgaactgct gtaccgccag
     1381 ggccaacagg gccttcgctc gtcggaccag ggcgaccagg ccaccgcgca gaccatcgtt
     1441 cactcgctgc tcgacctggg actcccgtcg ctcacgggcg gccggcgcgc gtatatcaac
     1501 ttcacgcgcg agatgctcct gggcggctat gtcgagctgt tgcccaagga cggtgtggtc
     1561 gttgagatcc tcgagaccgt ggacgtcgat gacgctgtgg tcggtgtgtg ctcggagctt
     1621 cagcgcgccg gctattgtct ggcgctcgac gatttcacgg
//
LOCUS       DAOSPL010001225         1659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_73542_length_1659_cov_2.326060, whole genome shotgun sequence.
ACCESSION   DAOSPL010001225 DAOSPL010000000
VERSION     DAOSPL010001225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73542_length_1659_cov_2.326060"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PLQ54_18455"
     CDS             complement(<1..334)
                     /locus_tag="PLQ54_18455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95299.1"
                     /translation="MTTARLNYAREEILADPAYATRIERKGVLLHGGYAADGTYLPPR
                     SVHRVPAIAAWAAQLEAAGHPSRVIEPEAVARAFIPNAAQAKMLLRNGASGAMTRILT
                     LVGVVEGFG"
     gene            complement(331..927)
                     /locus_tag="PLQ54_18460"
     CDS             complement(331..927)
                     /locus_tag="PLQ54_18460"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOS95300.1"
                     /translation="MREVTDNIADALPKAAAVFAARGLDGTRMEDIVEATGVPRPTLY
                     YHFSSKEEILAWLLQRLLRDLSIEIGGIVDRDEPARDRLRSIIAAYLQLFADHTQLCT
                     VLLTELGRITRIPALADAIWAAFHEPLGKVLDAGERDGSMRVSDHEMTASAIFGAVTM
                     VGLHHVVVGQPVDPPGLASQLDHMILRGLAPIPEGEQR"
     gene            complement(1031..>1659)
                     /locus_tag="PLQ54_18465"
     CDS             complement(1031..>1659)
                     /locus_tag="PLQ54_18465"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOS95301.1"
                     /translation="TFGIPEDMLGVFPAFYGAVQEWLHEIDESRVVPVDDGDILWLGG
                     RQLEAMVTPGHAAGHLSLWEPTSSRLLSGDHLLPSITPNPLVELDHESDLLRRRSLVE
                     YLDSLARFEALNPDVVLPGHGPAFTSVAQLVATTRRHHLGRADEILAHIERMGAPTAY
                     ELSRVVFPRIEGFEILLSVSEILGHVDLLVDGGSVVIDLGAPTRYLAT"
BASE COUNT          278 a          514 c          574 g          293 t
ORIGIN      
        1 tgccgaatcc ttcgacgaca ccgacgagtg tcaggatccg ggtcatggct cccgacgcgc
       61 cgttgcggag gagcatcttg gcctgcgcgg cgtttggtat gaacgcccgc gccacagcct
      121 cgggttcgat gacgcggctg gggtgacctg cggcttcgag ctgcgcagcc caggcggcga
      181 tggctgggac gcggtgcacc gaccggggcg gtaggtaggt gccgtcagcg gcatagccac
      241 cgtggagaag cacgcccttt cgttcgatgc gggtcgcgta agcgggatcg gcgaggatct
      301 cctcgcgtgc gtagttgagc cgggcagtcg tcatcgttgt tctccctcgg gtatcggcgc
      361 gagaccgcgc aggatcatgt gatcgagttg ggaagccagg ccgggggggt cgacgggttg
      421 gccgacgacg acgtggtgga ggccgaccat cgtgactgcc ccgaagatcg ccgacgcggt
      481 catctcgtga tcgctgactc gcatggaccc gtcgcgttcg cccgcgtcga gaaccttgcc
      541 gagcggctcg tggaatgccg cccagatcgc atcggcgagc gcggggatgc gcgtgatgcg
      601 accgagttcg gtgagcagca cggtgcacag ttgggtgtgg tcggcgaaca actggaggta
      661 tgcggcgatg atgctgcgga ggcggtcgcg tgccggctca tcccggtcga cgattcctcc
      721 gatctcgatg gacaggtccc gcagcaagcg ttggagcagc caggcgagga tctcctcttt
      781 ggaggagaag tggtaataga gggttggccg gggtacaccc gttgcctcga cgatgtcctc
      841 catgcgggtt ccgtccaggc cacgtgccgc gaacacggcg gcggccttag ggagcgcgtc
      901 ggcgatgttg tcggtgacct ctctcatcgc tcgcccccgg tcgatccatt ggtacccacc
      961 agctggtcgg gtgttactgc cgggttcgcc gtgtcggtgt cagccatgta agtcagtcca
     1021 gtcgcgtagg tcaggtcgcc agataccggg tcggggcacc gagatcgatc acaacgctgc
     1081 ccccgtcgac caagagatcg acgtgcccca agatctccga taccgacagc aggatctcga
     1141 atccttcgat ccgcgggaac accaccctgg acagctcgta ggccgtcggc gctcccatcc
     1201 tctcgatgtg ggccagaatc tcgtccgcac gacccagatg gtggcggcgg gttgtggcaa
     1261 ctagttgggc gaccgacgtg aatgctggcc cgtggcccgg cagaacgaca tctgggttga
     1321 gggcctcgaa gcgggcgagc gagtcgaggt actccacaag gctccggcgg cgcagtaggt
     1381 cgctctcgtg atcgagttcc acgagcggat tcggggtgat tgacggcaag aggtggtcgc
     1441 ccgacagcaa ccgcgatgag gtcggctccc acaacgacaa gtgaccggct gcgtggcccg
     1501 gcgtaaccat cgcctccagc tgccggccgc cgagccacag gatgtcgcca tcgtcgactg
     1561 gcaccaccct cgattcgtcg atctcgtgca gccactcctg aacggctccg tagaacgccg
     1621 ggaacacccc cagcatgtcc tctggaatcc cgaatgtgc
//
LOCUS       DAOSPL010001226         1658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_73601_length_1658_cov_2.959451, whole genome shotgun sequence.
ACCESSION   DAOSPL010001226 DAOSPL010000000
VERSION     DAOSPL010001226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73601_length_1658_cov_2.959451"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PLQ54_18470"
     CDS             complement(<1..613)
                     /locus_tag="PLQ54_18470"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS95302.1"
                     /translation="MNAYLSYTIRRLVLTVPILWGVFTVTFILFFIVPGDPARLIMGQ
                     HRDPAMQAAMQKQWGLDQPKHVQYWGFLKKMVVLDFGRSFTTKREVTQSILQRLPATA
                     VLGLPSLTLGLLLGVAAGLWSAMRANRAVDNTIRFVTFSLVSIPFFLLAICLQWLFGQ
                     HLRILPISGYINGPWGWLHLVLPVFAMTLGSFAGLTRLTRTAVL"
     gene            complement(610..>1658)
                     /locus_tag="PLQ54_18475"
     CDS             complement(610..>1658)
                     /locus_tag="PLQ54_18475"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOS95303.1"
                     /translation="PFMLQRWEPDVEILLRRNPNYWGRDDQGRRLPYVDAVHIVSRRD
                     PHTAFMEFEEGAADIAGVPDQDWDRVMTPDKRLRPAYEKRFVLETAPILTTQYYGFDM
                     SQPPFGGNKKLRQALNYAVDRESIIKQIGRGIGTPAYGPIPQGLPGYDPSNQRYRYDP
                     ERAKQLLAEAGYPEGKGLDEIVLYVSGVGDVADRNSVALMEQFRRIGVRIRLKQQPWP
                     LHLEAIDKREPRFFSLGWVADYPDAENFLALFTTRNHTPGPNATLYSNPEFDRLYDLA
                     MAELDEARRTELYRQAAAIIVEDCPWLFVTHGENLSLRQPWVRNFPPNPLGVYFYKNL
                     ILEGREPSRAGSAG"
BASE COUNT          286 a          533 c          570 g          269 t
ORIGIN      
        1 ccagcacggc ggtgcgggtg aggcgggtga gccccgcgaa ggaccccagt gtcatggcga
       61 acacgggcag caccaggtgt agccaacccc acgggccgtt gatgtagccc gagatgggca
      121 ggatccgcag gtgctggccg aacagccact ggaggcagat ggccagcagg aagaagggga
      181 tcgacaccag tgagaaggtc acgaaccgaa tggtgttgtc gaccgcgcga ttggcccgca
      241 tggccgacca caagcccgcc gccacaccca gcagcagccc cagtgtgagc gagggcagcc
      301 cgagcaccgc cgtggcgggg agacgttgga ggatcgactg cgtgacctcc cgcttcgtgg
      361 tgaacgagcg gccgaaatcc agcacaacca tcttcttcaa gaagccccag tactgcacgt
      421 gcttgggctg gtcgagaccc cactgcttct gcatggccgc ctgcatggcc gggtcccgat
      481 gctgccccat gatcaggcgc gccgggtcgc cgggcacgat gaagaacagg atgaaggtga
      541 ccgtgaacac gccccagagg atgggcacgg tcagcaccag acgccggatg gtgtagctga
      601 gatacgcgtt cacccggcgg atcccgccct gctcggctcc cggccctcga ggatcaggtt
      661 cttgtagaag tagacgccca gcgggttggg cgggaagttc ctcacccagg gctgccggag
      721 cgacaggttc tcgccgtggg tgacgaacag ccatgggcag tcctcgacga tgatggccgc
      781 cgcctgccgg tacagctcgg tgcgccgcgc ctcgtccagc tcggccatgg ccagatcgta
      841 cagccggtcg aactcgggat tcgagtacag ggtcgcgttg ggccccggcg tgtggttgcg
      901 ggtcgtgaag agggcgagga agttctcggc gtccgggtag tcggccaccc agcccaggct
      961 gaagaaccgg ggctcgcgct tgtcgatggc ctccaggtgc aagggccagg gctgctgctt
     1021 gaggcgaatg cgcacgccga tgcgccggaa ctgctccatc agcgccacac tgttgcggtc
     1081 ggccacatcg cccacgcccg acacatagag cacgatctcg tcgagcccct tgccttccgg
     1141 gtagcccgct tccgccagga gctgcttcgc cctttcgggg tcgtagcggt accgctggtt
     1201 gctcggatca tatccgggca gtccctgggg gatggggcca taggccggag tgccgatgcc
     1261 ccggccgatc tgcttgatga tcgactcgcg gtcgacggcg tagttgagcg cctgccggag
     1321 cttcttgttg ccgccgaagg gcggctggga catgtcgaat ccatagtact gcgtggtcag
     1381 gatgggcgcc gtctcgagca cgaaccgctt ctcataggcc ggtctgaggc gcttgtcggg
     1441 ggtcatcacc cggtcccagt cctgatcagg cacgccggcg atgtccgcag caccctcctc
     1501 gaactccatg aacgccgtgt gcgggtcgcg ccggctcacg atgtggaccg cgtcgacata
     1561 gggcaaccgt cgcccctggt cgtcgcggcc ccagtagttc gggttgcgcc gcaacaggat
     1621 ctccacgtcg ggttcccacc gctggagcat gaagggca
//
LOCUS       DAOSPL010001227         1657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_73694_length_1657_cov_1.958801, whole genome shotgun sequence.
ACCESSION   DAOSPL010001227 DAOSPL010000000
VERSION     DAOSPL010001227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73694_length_1657_cov_1.958801"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /gene="rimP"
                     /locus_tag="PLQ54_18480"
     CDS             complement(<1..453)
                     /gene="rimP"
                     /locus_tag="PLQ54_18480"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOS95304.1"
                     /translation="MSGHTRGPWRSRGRSTPARSRSPIRTQELFAFLAPRLAALGYEL
                     IELREGRAEGRRLIELLVDHPDGVGAYDCQRLAKHVGVLMDKMDPVEEQYMLQVSSPG
                     VDRPLKTVEHFERFAGHLAVVKFASEAECSQHGRRVKVRLAGVRDGRVA"
     gene            1126..>1657
                     /locus_tag="PLQ54_18485"
     CDS             1126..>1657
                     /locus_tag="PLQ54_18485"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOS95305.1"
                     /translation="MSSPDAPEAARRRRYTRIPISLRIEWRARRPGDAAVRPPAISQT
                     LDVAERGLRFVTDRADVGPGTYLDMTLYGPDTRGTAVVLEGIVTWVRPSGSDCEIGVR
                     LLSAPEDGLHALMMAAHEVLGEFSCVCRDVRFCGAAAEDCPAAVEGRNCWQIPHPPCC
                     YWSGGDRDCTHCPVSML"
BASE COUNT          254 a          593 c          535 g          275 t
ORIGIN      
        1 cgccacccgg ccgtcgcgca cccccgccag ccgaaccttg acccgccggc cgtgctggga
       61 gcattcagcc tccgatgcga acttgaccac ggccaagtgg ccggcgaacc gctcgaagtg
      121 ctccaccgtc ttcagaggcc ggtcaacccc tggcgacgac acttggagca tgtactgctc
      181 ctcgaccggg tccatcttgt ccatcagcac gcccacgtgc ttggcgagcc gctgacagtc
      241 gtacgcccct acaccgtccg gatgatcgac caacaactcg atcagccgcc gcccctcggc
      301 ccggccctcg cgcagctcga tcagctcata gccgagggcg gccagccgcg gggccaggaa
      361 cgcaaagagc tcctgcgtgc ggatgggcga ccgcgaccgc gccggcgtgg accggccacg
      421 cgaacgccaa gggccgcggg tatgcccgct catcgcccgg ccacccgccc ctccgaacaa
      481 caaagagtgg gtccaggccc actctttgac cggcgaaccc caacaactcc gggcactata
      541 tcacaagtgg ccttcgcgcc gcaagaacgc cttcgcggag ccgcggagac ctcccgcagc
      601 aagggtcctc cagccgcttc ccagcgcagc cctcagtgaa ggggcaagcc ccgacgatcg
      661 ccacaacctt gtggcctctc gtgtgacgcc cgtgacgctt gctctcgcga gttacccatg
      721 gcgtgtgtct gagacgacgg tgcgattccc cgaaaagctg tggaatccgg cgtcacatgc
      781 gtgacacttc gcggccgaat ccccgtcccg ggtttggcgc ggtccgcgtg cccgtgggtc
      841 tggttcgcgc gtcatcgccc ggcagcccag cggccgtctc gtccagaaac gcgcgcgcaa
      901 tactattata ccagttatga gctgtattgt caagcacttt gtgcgagcag atgtgtgtac
      961 tcttggctgc cggggacggc ggacggcatc gatcccgcga gggtgtccgg tctcatgtgc
     1021 ccgaggggcg cctgcggcgc tgttggccgt gctggcatgc cccgcggcac gttatactgg
     1081 cccagccgtc cgctggtgcc cgctatgctg cgaggaggtc tggttgtgag ctcgcctgac
     1141 gcaccggagg ctgcccgccg ccggcggtac acgcgcatcc ccatcagtct gagaatcgag
     1201 tggcgggcgc gtcggccggg cgatgcggcg gtgcgacctc ccgcgatctc acagactctt
     1261 gacgtcgccg aacgcggcct ccgcttcgtc accgaccgcg ccgatgtcgg gccggggacc
     1321 tacctggaca tgacgctcta cgggcccgac acgcgtggca ccgcagtggt gcttgagggc
     1381 atcgtcacat gggtacggcc gtcgggcagc gattgcgaga tcggcgttcg gttgttgtca
     1441 gcgccggagg atgggctcca cgcgctgatg atggccgcgc acgaggtcct gggcgagttc
     1501 agctgcgtct gccgcgatgt ccgcttctgc ggtgcggccg ctgaagactg cccggcggcc
     1561 gtcgagggcc gcaactgctg gcagatcccc cacccgccct gctgctactg gtccgggggc
     1621 gaccgcgact gcacgcattg ccctgtgtcg atgctgt
//
LOCUS       DAOSPL010001228         1654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_73848_length_1654_cov_3.026892, whole genome shotgun sequence.
ACCESSION   DAOSPL010001228 DAOSPL010000000
VERSION     DAOSPL010001228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73848_length_1654_cov_3.026892"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1560
                     /locus_tag="PLQ54_18490"
     CDS             <1..1560
                     /locus_tag="PLQ54_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95306.1"
                     /translation="KLRVRGIAPERIAKLTVRFPLRRESARFVLRQLVQDVRPLTGFG
                     WEGPAGPVWLGGHDRGLAFAYDTPLFLSAHRRSQVQVVEEADRTWLCLNLVDAPGQVT
                     DEGQVFRFFLTPTPTKPVSLRKDGLFHTALWFENWSDYQGYPDLAKLPEVRRRSDEAH
                     ARGVPQVLYFSQVLAENSPGFAECRSEFLVPPGMAWYRRAYDPGRDVPCWVSCPRGPF
                     GDRLLDGMERLAAQGGIDGVYMDGLSVPWDCTNPAHAACAQPVTPMWDRDELTPLVAT
                     RAFLKRVRGIFDQRGHPRLFAHTGGAINIETLSLYDGYYEGEQLARYRPGFRLPLHQA
                     AVGYCGRPWGFRTDAIPVAYGTRRLMALAALHDTEVGGDSTELERCMYTDFQSDAAVD
                     YRPYWRTQPHVRPVAGNVIYSYYRKPGAALLVVSNLTWDTQQAELDVSGLFAGRPIHA
                     IDLDRRVPVAVAGSRLGLSLKPHRFAAIRLEPGAGEEAAPPFRRPNRPAVHIAEYRDA
                     GRRQPGRPARS"
     gene            1520..>1654
                     /locus_tag="PLQ54_18495"
     CDS             1520..>1654
                     /locus_tag="PLQ54_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HOS95307.1"
                     /translation="MLADGNPADLLEVKGAALWAEKRGPKNASLEQCDFGLGPGKLDG
                     A"
BASE COUNT          245 a          601 c          547 g          261 t
ORIGIN      
        1 aagctgcgcg ttcgtggcat cgcgccggag cgcatcgcga agctgaccgt tcggttccca
       61 ctgcgccggg agagcgcccg tttcgttctc cggcagctcg tgcaggacgt ccggccgctc
      121 acgggattcg gctgggaggg ccccgccggg cccgtatggc tcggcgggca cgaccgcggc
      181 ctggccttcg cctacgacac gccgctgttc ttgtcggcgc acaggcggtc gcaggtgcag
      241 gtcgtggagg aggccgatcg cacatggctc tgcctcaacc tggtcgacgc accgggccag
      301 gtaaccgacg agggacaggt gttccggttc ttcctcacgc cgacgccaac gaagccggtg
      361 tcgctccgca aggatggcct gttccacacc gcgctgtggt tcgagaactg gtcggactac
      421 cagggctacc cggatctcgc caagctcccc gaggtccggc ggcgttccga cgaagcccac
      481 gcgcgcggag tgccgcaggt cctctacttc agccaggtgc ttgctgagaa ctcgcccggc
      541 ttcgccgagt gccgatcaga gttcctggta ccgccaggaa tggcctggta ccgccgggcc
      601 tacgaccccg ggcgcgatgt cccgtgctgg gtgagctgcc cgcgcgggcc gtttggcgat
      661 cgattgctcg acggcatgga acgcctggcc gcccagggcg gcatcgacgg cgtctacatg
      721 gacggtctct cggtcccgtg ggactgcacc aaccccgcgc acgcggcgtg cgcgcagccg
      781 gtcaccccca tgtgggaccg cgacgaactg acgcccctgg tggctacgcg ggctttcctg
      841 aaacgggtgc ggggcatctt cgaccagcgc ggacacccgc gactcttcgc ccacaccggc
      901 ggcgctatca acatcgagac gctctctttg tacgacggct actacgaggg cgagcagctc
      961 gctcgctacc ggcccggctt ccgtctcccg ctccaccagg cagctgtggg ctactgtggc
     1021 cggccctggg gcttccgtac cgacgccatc cccgtcgcct acggcacccg gcgactgatg
     1081 gccctggccg cactgcacga caccgaggta gggggcgaca gcaccgaact cgagcggtgc
     1141 atgtacacgg actttcagag cgacgccgcc gtcgactatc gtccctactg gcgcacgcag
     1201 ccacacgtcc gtcccgtggc cggcaacgtg atctactcct actatcgcaa gcccggcgcc
     1261 gcactgctgg tggtcagcaa cctgacctgg gacactcagc aggccgagct cgacgtgtcc
     1321 ggtctgttcg cgggccgacc gatccacgcg atcgacctcg atcggagggt acctgtggcc
     1381 gtcgcgggca gtcgcctggg cctctcgctc aagccccacc ggttcgcggc gatccggctc
     1441 gagcccggcg ccggcgagga ggccgcgcct ccgttccggc gccccaaccg cccggcagta
     1501 cacatcgccg agtaccgcga tgctggccga cggcaacccg gccgacctgc tcgaagttaa
     1561 gggtgctgcg ttgtgggccg agaagcgcgg tccgaagaac gcctcgctcg aacaatgcga
     1621 cttcggcctc ggccccggca agctggacgg ggcc
//
LOCUS       DAOSPL010001229         1653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_73926_length_1653_cov_2.354819, whole genome shotgun sequence.
ACCESSION   DAOSPL010001229 DAOSPL010000000
VERSION     DAOSPL010001229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73926_length_1653_cov_2.354819"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..730
                     /locus_tag="PLQ54_18500"
     CDS             299..730
                     /locus_tag="PLQ54_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95308.1"
                     /translation="MMTSRERMVTAMLNKQPDMVPVAPDISNMIPCRLTGKPFWDIYL
                     YNDPPLWKAYIQAAKHFGFDGWLPSVCAQTRIRNAAIVGRTAERIYLQSYREENGKRV
                     WAPHVTVYYVADPPTYGVPVEKIGLPASPSSGKTSSTSPRS"
     gene            766..1605
                     /locus_tag="PLQ54_18505"
     CDS             766..1605
                     /locus_tag="PLQ54_18505"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS95309.1"
                     /translation="MGDDGVLGLHVGIPGLYNDAGIFEYYDNPNLVRDRARAYEETVE
                     QRTREVCEMGPDFVFTGYSGCLTFQTPAIFRDLGLPSLQIITRVAKEYGLPSQVHSCG
                     PEAKLVEIAAKESDLTSINPLERPPMGDCRLDEIKRDFGADISLMGNLHTTEVMLHGT
                     PDLVRRESRWAIDVAAEGGGFILSTGDQCGRDTPDEKPLRHDGDGARVGEVLRNAAAR
                     SGPLPQCGLPAQPREQYAVVIDRTEGRAALRSTVHGPGPIWSRRRHSRAASRSSISHA
                     LRM"
BASE COUNT          301 a          579 c          529 g          244 t
ORIGIN      
        1 agtgtcccgt cgtgcccgtt gcccgaggcg tccgcagtat cgccatcgaa ccgccattcg
       61 agcaccaggt cactgggccc ggcatcggcc gcccataccg cctgaagccc gaccggaatg
      121 cccagcaccg acaccgccgt catccacgca gtcgttcgca ccgcacgcac ctccaatagc
      181 agccgcccac tgcattccgc gggaagcacg aaggggcctt ggaggcgcgt cggcgcagga
      241 ggctcccgcg gccggctcgc cgaacgctcc cggcgccctg cgcggcgatc ggaggcccat
      301 gatgacctcg cgcgaacgta tggtcacggc catgctcaac aagcagcccg acatggtgcc
      361 cgtggcgccc gatatctcga acatgattcc ctgccggctg accggcaagc cgttctggga
      421 catctacctc tacaatgacc cgccactctg gaaagcctac atccaggctg ccaaacactt
      481 cggcttcgat ggctggttgc cgagcgtctg cgcccagacg cggatccgca acgccgccat
      541 cgtgggacga actgccgagc gcatctacct tcaaagctac cgggaggaga acggtaagcg
      601 cgtctgggcc ccgcatgtga cggtctacta cgtagccgat ccgcccacct acggcgtccc
      661 ggtcgagaag atcggcctcc ccgccagccc gagcagtggg aagacttcgt cgacgagtcc
      721 gaggagctga ccgaggagca agccctgcgc gccgcgcggg agctgatggg tgacgacggc
      781 gtgctcggcc tgcatgtcgg catcccgggc ctctacaacg atgccggcat cttcgagtac
      841 tacgacaacc cgaacctggt gcgcgaccgg gctcgggcct acgaggagac cgtcgagcag
      901 cgcacgcgtg aggtctgtga gatgggcccc gacttcgtgt tcaccggcta ctcgggttgc
      961 ctcaccttcc agacgcccgc catcttccgc gacctggggc tgcccagcct gcagatcatc
     1021 acgcgcgtcg ccaaggagta cggcctgccc agccaggtcc actcgtgtgg ccccgaagca
     1081 aagctcgtgg agatcgcggc caaggagtcg gatctgacca gcatcaatcc gctcgagcgc
     1141 ccgcccatgg gcgactgccg gctcgatgag atcaaacgcg actttggggc agatatcagc
     1201 ctgatgggca acctccacac caccgaggtc atgctccacg gcacgccgga tctggtccgc
     1261 cgcgagagca ggtgggccat cgacgtcgcc gccgagggcg gcgggttcat cctgtcgacc
     1321 ggcgaccagt gcggccgcga tacgcccgac gagaaacctc tacgccatga tggagacggc
     1381 gcgcgagttg gggaagtatt gaggaacgcg gccgcgcgat ccgggccgct accgcagtgc
     1441 ggcctcccag cgcagccccg agagcagtac gcagtagtca tcgaccgaac agagggtcgg
     1501 gccgcactcc gcagcaccgt gcacgggccg gggccgatat ggtcgagacg ccgtcacagc
     1561 cgggcggcct cacggtcgag tatctcccat gccttgcgga tgtagtagcg gtacgcctcg
     1621 agcggcgtgc cgttggggat gcgatggtcg aga
//
LOCUS       DAOSPL010001230         1650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_74092_length_1650_cov_3.319749, whole genome shotgun sequence.
ACCESSION   DAOSPL010001230 DAOSPL010000000
VERSION     DAOSPL010001230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74092_length_1650_cov_3.319749"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PLQ54_18510"
     CDS             <1..538
                     /locus_tag="PLQ54_18510"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOS95310.1"
                     /translation="VWAVVLPGRGASGAEGVWFGLGAGLKVVAGMCVGLVFLSCTTAE
                     EMSRGLVGLGVPFRFAFVIAMALRLVPTVVSTALDIIDAQRSRGLDLESGGLIRRARA
                     HVPLLAPMLLSTLRGADQLSMALECRALGARRRRTWLMASELGWRDVAVMGGVVAYFG
                     LAVALRIAGCGVWSAPGR"
     gene            544..>1650
                     /locus_tag="PLQ54_18515"
     CDS             544..>1650
                     /locus_tag="PLQ54_18515"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOS95311.1"
                     /translation="MRGLPEGLVADSERRPRALQRVEVADLDAMSAMFRVTFVCVALA
                     FAVQSGQMARGNLYAITLLFLCLQAVVLWSSARHMKADRLRPAVMGIELLVFCAWMRE
                     TPSYAPVLFTLFYLTVLMAAMRYGLVGSMVSAVAAIGLYLWQVGDADGIAATCQRMGS
                     SHFVPLLIVAAITGAMARVRDQEREARFAQERVMVEYHQRVELARSVQEFSLPTTLPV
                     IAGYDVAKAFDIADYAVGGGDYYDIRVGPDGAFVLCVADIAGKTISGVAKLPLIRAGL
                     AVASQMLETPGAIARCLNEVMCSTIEEDSFIAASLARLEPDTGRLTYTTAGQVPPLIV
                     RRGGQVEWLELGGPALGIQADAVYREGECGMGPGD"
BASE COUNT          219 a          511 c          609 g          311 t
ORIGIN      
        1 cgtctgggcc gtggtgctac cgggtcgggg cgcatccggg gccgaaggag tatggttcgg
       61 tctcggcgcc gggctcaagg tggttgccgg catgtgcgtg ggactggtct tcctatcatg
      121 caccacagcc gaagagatgt cgcgggggct ggtggggctc ggagtgccgt tccggttcgc
      181 cttcgtgatc gccatggcgt tgcgcctggt gcccaccgtg gtgtcgacgg cactcgacat
      241 catcgatgcc cagagatctc gcgggctgga tctggagtcg ggcggcctga ttcggcgagc
      301 ccgtgcccat gtgccgctct tggctcccat gttgctctcg acactgcgcg gcgccgacca
      361 gctctccatg gctctcgagt gccgggctct tggcgcccgg cgtcgacgta cctggctgat
      421 ggcctccgaa ctgggctggc gggacgtagc ggtgatgggc ggtgtggtgg catacttcgg
      481 actggcggtc gcgctgcgca tcgctgggtg cggcgtctgg agcgcaccgg gtaggtgaag
      541 tcgatgcgcg ggctgccgga ggggctcgtg gccgattccg agcgcagacc cagagcgctg
      601 caacgcgtcg aggtggcgga cctcgacgcc atgtccgcca tgttccgcgt gacattcgtg
      661 tgcgtcgcgc tggccttcgc cgtccagtcg ggccagatgg cgcgcgggaa cctctacgcg
      721 atcaccctgc tgttcctgtg tctacaggcg gtggtgctgt ggtcgtcggc gcggcacatg
      781 aaggccgacc gactgcgtcc tgccgtcatg ggcatcgagt tgctggtctt ctgcgcatgg
      841 atgcgcgaga cgcccagtta cgcgccggtg ctcttcacgc tgttctacct gacggtgctc
      901 atggcggcca tgcggtacgg gctggtggga agcatggtgt cggccgtggc ggccattggg
      961 ctgtacctgt ggcaggttgg cgacgcggac ggcatcgccg ctacgtgcca gcggatgggg
     1021 tcgagccact tcgtgccgct actgatcgtg gcggccatca caggggcgat ggcacgggtg
     1081 cgggaccagg agcgcgaggc gcgtttcgcg caggagcgcg tgatggtcga gtaccaccag
     1141 cgcgtggaac tggcccgttc ggtccaggag ttctccctgc ccaccacgct gcccgtcatc
     1201 gccgggtacg atgtcgcgaa agccttcgat atcgccgact acgccgtcgg gggcggggac
     1261 tactacgaca tccgggtggg tcccgatggc gccttcgtgc tctgcgtggc cgatatcgcc
     1321 ggcaagacga tctccggcgt ggcgaaactc ccgctgatcc gggccggtct ggccgtggcc
     1381 agccagatgc tcgaaacacc cggtgccatc gcacgatgcc tcaacgaggt gatgtgctca
     1441 acgatcgagg aggactcgtt catcgcggca tcgctggcgc ggctcgaacc ggatacgggg
     1501 cgcctgacct atacgaccgc gggccaggtt ccccccctaa tcgttcggcg cggtggtcag
     1561 gtggagtggc tcgagctcgg cggacccgcc ctcgggattc aggccgatgc cgtctaccgg
     1621 gaaggcgagt gcggtatggg cccgggggac
//
LOCUS       DAOSPL010001231         1649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_74184_length_1649_cov_1.826223, whole genome shotgun sequence.
ACCESSION   DAOSPL010001231 DAOSPL010000000
VERSION     DAOSPL010001231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74184_length_1649_cov_1.826223"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLQ54_18520"
     CDS             <1..313
                     /locus_tag="PLQ54_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95312.1"
                     /translation="FVIVSRRDDGQLGVSLVDPHGTFLADGRAKVRALADYAGRFGGV
                     AADGEPDRAPLVRVDAIAEVDGTVRVLDLLDAAVRQAVLDFEGSDLTALYASPHARDF
                     K"
     gene            complement(383..940)
                     /locus_tag="PLQ54_18525"
     CDS             complement(383..940)
                     /locus_tag="PLQ54_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95313.1"
                     /translation="MSLTTCSWCGSDEHVDEYDPVEDIASCSGPGHPEPRMFQPKLER
                     AIRRETDDKWTYLCDSIAAGYGLYDLLPELLVAGKWFDTNVIELRFALAHPEIYAELL
                     DRWGHVSQGSRKYSTSSFLGSTLGALCRAANVTHKSGHGSGRFSYNRWVGFWTIEPVP
                     ADAVDVGWSDYAVANGLDPATWPLA"
     gene            complement(937..1428)
                     /locus_tag="PLQ54_18530"
     CDS             complement(937..1428)
                     /locus_tag="PLQ54_18530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95314.1"
                     /translation="MTLSITPITGTELSVFTIVTRSSQERRVRRFHHELEDVTTGEWT
                     PLSRSAAREKYPNAWDAPQWKESDLDRVEWVQSKANAQTWKAIEHIARTGNVTGDEVA
                     AAVGYTDAMAWKSALPHLSAYCLRIGRRPLWDVERSTGTFRYYVSPTVGALLAELEGS
                     EEA"
BASE COUNT          265 a          515 c          570 g          299 t
ORIGIN      
        1 cttcgtgatc gtctcccgac gcgacgacgg acagctcggg gtgtcgctcg tcgatccgca
       61 cggaacgttt ctcgcggatg gtcgggccaa ggttcgagcg ctggcggact acgccggtcg
      121 cttcggcgga gtcgcagcag acggtgagcc tgaccgggcg ccgttggtcc gagtcgacgc
      181 gatcgccgag gtcgacggaa ctgtgcgcgt gctcgacctc ctcgacgcag cggtcagaca
      241 ggccgtgctc gacttcgagg gcagcgacct tacggccctc tacgcctcgc cccacgcccg
      301 cgacttcaag tagcgcggcc gactccggcc caccgccgac cgtgcggtag atgctcgacc
      361 agcgtggttg accgagctgc gatcaggcga gtggccaagt cgcagggtcg aggccgttcg
      421 ccacggcgta gtcggaccag ccgacatcga ccgcatctgc ggggaccggt tcgatggtcc
      481 agaagccgac ccaccggttg tagctgaacc tcccactgcc gtggccggac ttatgagtga
      541 cgttcgcggc acggcagagc gctccaagcg tcgagccgag gaacgaggac gtgctgtact
      601 tccttgagcc ctgcgacacg tggccccatc ggtcgagcaa ctcggcatag atctcagggt
      661 gggcgagagc gaagcggagc tcgatgacgt tcgtgtcgaa ccacttgccg gcgaccagca
      721 gttcgggaag caggtcgtac aggccgtacc ccgcggcgat gctgtcgcag aggtatgtcc
      781 acttgtcgtc ggtctcgcgt cggatggccc gctcgagctt cggctggaac atgcggggct
      841 cggggtggcc tggcccggag cacgaagcga tgtcctcgac ggggtcatac tcgtcgacgt
      901 gctcgtcgct gccacaccag gagcacgtgg tgaggctcat gcttcctccg atccctcgag
      961 ctcggcgagc agcgcaccga cggtgggaga gacgtagtag cggaacgtcc cagttgaacg
     1021 ctcgacgtcc cacagcggcc gccggccgat gcgaaggcag tatgccgaca ggtgtgggag
     1081 ggcggacttc catgccatcg cgtcggtgta tccgacggcg gcggccactt cgtcgccggt
     1141 gacgttgccg gtgcgggcga tgtgttcgat ggccttccag gtctgcgcgt tggccttgga
     1201 ctggacccac tcgacgcggt cgaggtcgga ctccttccac tgcggggcat cccaggcgtt
     1261 ggggtacttc tctcgtgcgg cgctccggga gagcggtgtc cactccccgg tggttacgtc
     1321 ttcgagctcg tggtggaacc gtcgaactcg gcgctcctga ctcgaccgcg tgacgatcgt
     1381 gaacacgctg agttcggtgc cagtgatggg tgtgatggac agggtcatgg gatctccgtt
     1441 tcatgaggtg atatgagacg atcataacag gtcataagag aatggcaact gctaatcgct
     1501 ctgcgcgcgc ttagcagggg tgacgattcg cgtcggggtg gtcccggtgc tcgacatcgt
     1561 cgaggagccc cacatggagt cgatcacccg tcagaccctc tccgtcgctg aggcggcgga
     1621 ggtgctcggt atctcccgag cgcacgcct
//
LOCUS       DAOSPL010001232         1648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_74235_length_1648_cov_2.197112, whole genome shotgun sequence.
ACCESSION   DAOSPL010001232 DAOSPL010000000
VERSION     DAOSPL010001232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74235_length_1648_cov_2.197112"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PLQ54_18535"
     CDS             <1..424
                     /locus_tag="PLQ54_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HOS95315.1"
                     /translation="RRQDDGIDPIFRQYQAGQDQTTLLTTAIRGLKDDGYDLSEIVVL
                     SPLRDASTAATTRDPWLRQILKPADGLAARAGQVRYSTIHAFKGLEAPAVVVTDLDAA
                     TTTDNFDSLLYVGLTRATDRLIALIEARTLRHAIGGTA"
     gene            421..>1648
                     /locus_tag="PLQ54_18540"
     CDS             421..>1648
                     /locus_tag="PLQ54_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HOS95316.1"
                     /translation="MSGLEARAIVEAELRRELFGPLGSEDPAGKPVDCSSGTLTFATA
                     EEGRGQFHDSSTLQEILTIGTPLTRYGIGVLYSGASIGGTAIGESGDEDVDLTGIPGV
                     ARNEEDPDGPPVEIQGSLRHDEADSDDFDLTDANSFKPSAMAISFQCRIPVDGSLSVT
                     VTGAHYEKLSVHIPGVSKLVDWWRRRPFTLTGSVPGSVLVEETHRLKIVDTAPEGDPP
                     GIAPTTRVFSRPVPGVDDREMRLVTVAVVNQVMGTGPSSALFQMGFTVAASGGLVIEP
                     YPEVELPDRDQEGQSIDLLYRNKRTYAIGHGCAAEWEGGAGAPVPLVRAVALPAYEVV
                     SLTPNIYLTNEDGSFSFDAEGQRQAVTVSMKELAVGTASGHAQVETVLRLYGEWIDAR
                     RAEIDDLPTRFRDAARR"
BASE COUNT          304 a          552 c          519 g          273 t
ORIGIN      
        1 ccgtcgacaa gacgatggca tcgatcccat cttccgtcag taccaggccg gtcaagacca
       61 gacgacgctc ctcaccacag cgattcgcgg gctcaaagac gacggttatg acctcagcga
      121 gatcgtcgtc ctcagtcctc tgcgcgacgc gtccaccgca gccaccacac gcgacccgtg
      181 gctccggcag atcttgaagc ctgccgatgg gctcgccgcc cgtgccggtc aggtgcggta
      241 ctccaccatc catgccttca agggactcga ggcccccgcc gtcgtggtga cggacctcga
      301 tgccgccact accacggaca acttcgactc attgctctac gtcgggctca cccgagccac
      361 cgaccgcctg atcgcactca tcgaagccag gacactccgt cacgctatag gaggaactgc
      421 atgagcggac ttgaagcccg tgccatagtc gaagccgaac tccggcgcga actgttcggc
      481 cccctcggct cggaagaccc ggccgggaag ccagtcgact gttcgagtgg cacgctcacc
      541 ttcgctacgg ccgaagaggg ccgaggacag ttccacgaca gctccacgct gcaggagatt
      601 ctgaccatcg ggacgcctct cacacgttac ggcattggcg tgctttacag cggagcctcc
      661 atcgggggca cggcaatagg ggagtcaggc gatgaggatg tcgacctgac gggcatcccc
      721 ggtgttgccc gcaacgaaga ggaccccgac ggaccgcctg tcgagatcca aggctcgctt
      781 cggcatgacg aggccgactc cgacgacttc gacctgaccg acgcgaacag cttcaagccc
      841 tcggcgatgg cgatctcgtt ccagtgccgc ataccggtcg acggctcact gtccgtcacc
      901 gtgaccggtg cccattacga gaagctgagc gtccacatcc cgggggtatc gaagctggtc
      961 gactggtggc gccgccgccc cttcacgctt accgggtcgg taccgggaag tgtcctggtc
     1021 gaggagaccc atcgcctcaa gatcgtcgac accgcgccgg aaggcgaccc acctggcatc
     1081 gcgccgacaa cgcgtgtctt cagccgcccg gtacctggag tcgacgatcg ggaaatgcgc
     1141 ctggtgacgg tggcggtggt caatcaggtg atgggcactg ggccgagcag cgccctgttc
     1201 cagatgggct tcaccgtcgc cgcctctggc ggtctggtta tcgagccgta ccccgaggtc
     1261 gagctacccg accgtgacca ggaagggcag tcgatcgatc tgctgtaccg caacaagcgc
     1321 acctacgcca tcgggcatgg ttgcgccgcc gagtgggaag gcggtgctgg tgcacccgtc
     1381 ccgctggtgc gtgctgtggc gttgccggcc tacgaggtgg tcagcctgac cccgaacatc
     1441 tatctgacca atgaggacgg gagtttcagc ttcgacgcgg aagggcagcg tcaggcggtc
     1501 accgtcagca tgaaggagct ggcggttggg accgccagcg ggcacgccca ggtggaaact
     1561 gtgctgcgtc tgtatggtga atggatcgat gcccggaggg ccgagattga cgacctgccc
     1621 acacgcttcc gcgacgcggc ccgtcggc
//
LOCUS       DAOSPL010001233         1647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_74273_length_1647_cov_3.353643, whole genome shotgun sequence.
ACCESSION   DAOSPL010001233 DAOSPL010000000
VERSION     DAOSPL010001233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74273_length_1647_cov_3.353643"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLQ54_18545"
     CDS             complement(<1..223)
                     /locus_tag="PLQ54_18545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95317.1"
                     /translation="MGTRKYAFLASLGFAPMEPARVVGTLAELGYDGVEWTLAHFHPQ
                     AHTPAQLAAVVEATRAGGLGISELVVQQDM"
     gene            complement(416..>1647)
                     /locus_tag="PLQ54_18550"
     CDS             complement(416..>1647)
                     /locus_tag="PLQ54_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95318.1"
                     /translation="RAGTGKSALAQAQVVAEADSWFLRCTGLISIAAIRHLMGDYPGA
                     KAMIEDAEALLAGAPANPRETAYLQAYLAGNRANIHLDTGEFAEALDASTQMSASAAM
                     ADSRSQSLESLINTGLCHLRLGSMADSWRCLTQARQGASLAGDRLREATANWALSEWY
                     VCAGLCGNAVVHAEAARSLASSSQARFALIQACLALGAAHLEARQPERAIEPLDAAIA
                     AARALRAAHQEASGEVLLARVALHQGRAKDAADALSRCLSRARAMGALGLAADAQSAW
                     ASALLRLGHTEAACEEAVAAAAAAEQQGAVHTLWTAGHVAAKAHAALGRTDEALACFR
                     TAVGTVEEMWWPLWRIGFAEVKDIKQSVVDLYLDYIRFATASGCPDEVRRVLAMSPWS
                     FLRERCEREGLLSVVFE"
BASE COUNT          261 a          594 c          568 g          224 t
ORIGIN      
        1 ccatgtcctg ctgcaccacc agctcggaaa tgcccaatcc tcccgctctc gtggcctcga
       61 ccacggccgc cagttgggcc ggcgtgtgcg cctgtggatg gaagtgggcc agagtccact
      121 cgacgccgtc gtaaccgagc tcggcaaggg tgcccaccac ccgggcgggc tccatggggg
      181 cgaacccaag gctggcgagg aacgcgtact tgcgggtgcc catagaccgc agacctccag
      241 tagtactcgg tgcgggttcg gccgccggga tcacggccgc ggtggggttc ccctttggac
      301 tcgcggggac ctaccatcgg gacgccaagg gccatgccgc cagccgcgca gcagactgca
      361 gacgcatggg ggaaacgcgc ggccctccgg cgccgcacgg ctcggccatg ccccgctact
      421 cgaacaccac cgagaggagg ccttcccgct cgcagcgttc acgaaggaag gaccagggcg
      481 acatggccag cacccgccgc acctcatcgg ggcacccgga ggccgtggcg aagcggatgt
      541 agtcaagata caggtcgacc acggactgct tgatgtcctt gacctccgcg aagccaatgc
      601 gccacagcgg ccaccacatc tcctcgacgg tgccgaccgc ggtccggaag caggccagcg
      661 cctcgtcggt cctccccagg gccgcgtggg ccttggcggc cacgtgaccg gcggtccaca
      721 gtgtgtgaac ggcgccctgt tgctcggcgg cggccgcggc agccacggcc tcctcgcaag
      781 ccgcctcagt gtgtccaagc cgaagcaggg cggacgccca ggcagactgc gcgtcggcgg
      841 caaggccaag agcgcccatc gctctggcac gggacaggca ccggctcagc gcatcggccg
      901 catccttcgc gcgcccctga tggagagcga cgcgggccag cagaacctcg cccgaggcct
      961 cctggtgtgc ggcccgaagc gcccgcgccg ccgcgatggc ggcgtccagt ggctcgatcg
     1021 cccgctcagg ctggcgcgcc tcgaggtggg ccgcgcccag cgcgaggcag gcttggatca
     1081 gcgcgaaacg cgcctgactc gaggaggcca ggctccgcgc cgcctccgca tggacgaccg
     1141 cattgccaca cagtcccgcg cagacgtacc actcggagag agcccagttc gccgtcgcct
     1201 cgcgcagccg gtcaccggcc agtgaagcgc cctggcgagc ctgggtcaag catcgccagg
     1261 aatcggccat gctccccaga cggaggtggc acaagccggt gttgatgagg gattcgaggc
     1321 tctgggaccg gctatcggcc atggcggccg aggccgacat ttgggtcgac gcgtccagag
     1381 cttcggcgaa ctcgccggta tccaggtgaa tgttggcccg gttcccggcg aggtaggcct
     1441 gcaggtaggc cgtttcccgc ggattcgccg gcgcaccggc gaggagcgcc tcggcgtcct
     1501 cgatcatcgc tttggccccg gggtagtcac ccatgaggtg gcggatggcc gcgatgctga
     1561 tcagccccgt gcatcggagg aaccagctat cggcctcggc aaccacctgg gcctgggcca
     1621 gcgcgctctt gccggtgccc gcccgcc
//
LOCUS       DAOSPL010001234         1646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_74335_length_1646_cov_3.245129, whole genome shotgun sequence.
ACCESSION   DAOSPL010001234 DAOSPL010000000
VERSION     DAOSPL010001234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74335_length_1646_cov_3.245129"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLQ54_18555"
     CDS             <1..260
                     /locus_tag="PLQ54_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95319.1"
                     /translation="WEFPWFVAQVSYHSPQQPRFSNPREGQRLVLERRIALPGPDTDT
                     LTGDHRDMDGEGIHFSPKGLKAHGEMWAKDVGDYLEAVLRR"
     gene            complement(362..>1646)
                     /locus_tag="PLQ54_18560"
     CDS             complement(362..>1646)
                     /locus_tag="PLQ54_18560"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS95320.1"
                     /translation="AARAGADTLLVERCHYPGGVATAGLVCNCTNFFVTQSGNRAVRG
                     LATRILDRLVAAGGAMPHYARPEQPQIPYDPETLKWILIEMLREAGVRSRYGLFVSRV
                     TGTGSEIDAVVCEGKGGPVAFRARQFVDATGDLDLFALSDGPFDVLEGFSTLQFRMGG
                     VDIDGIMDWLERNPDCYAAEKDVPTSLEDTLRNWREFGVFHLPHYGGMAISVVREAVE
                     RGELPDRFGEHVGDLWAMGMFASRRDQGRVLINSCACRGDELDPVRRSECEEESRLAA
                     HRLAEFLRAHFPGFEQAYLAETAPEVATRFTRRLRGMYTLTRDEQSAGTVFPDCVGRI
                     TELDRRFSPCRRYAQAGEVPFGCLVSEAPANAICGSGKSASTEPRGLLRGQVGCMVVG
                     EAAGTAAAVAARSNVPVRDVDVSRLQQALIAADAL"
BASE COUNT          284 a          567 c          557 g          238 t
ORIGIN      
        1 ggtgggagtt cccctggttc gtggcccagg tgtcctacca cagcccgcag cagcctcggt
       61 tcagcaaccc ccgagaggga cagcggctgg tcctggagcg gcgcatcgcg ctaccggggc
      121 ccgataccga cacactgacg ggcgaccatc gggacatgga tggcgagggc attcatttca
      181 gcccgaaggg gctcaaggcc catggagaga tgtgggcgaa ggatgtggga gactacctgg
      241 aagccgtgtt gaggcgttag ggaacgcaag ggccgggggt ggggcgagcg tgttgcccgc
      301 cggccctgtc acaggcagac tcagcagggc ggccgggctc tgcgggacgg agagcaagcc
      361 gctagagcgc gtctgcggcg atgagggcct gctgcaaccg ggagacgtcg acatcgcgca
      421 ccggcacgtt gctccgcgcc gccacggctg ccgccgtgcc cgctgcctcc cccacgacca
      481 tgcacccgac ctggccgcgc aagagtcccc tcggttcagt ggacgcgctc ttgcccgacc
      541 cgcagatcgc gttggcaggc gcctcggaca ccaggcagcc gaaggggacc tcgccagcct
      601 gggcatagcg gcggcagggc gagaaccggc ggtcgagctc ggtgatccgg cccacacagt
      661 ccgggaagac cgtgccagct gactgctcgt cgcgcgtgag cgtgtacatg cccctgagtc
      721 ggcgcgtgaa ccgtgtggcc acctcgggcg ccgtctctgc caggtatgcc tgctcgaagc
      781 ccgggaagtg cgcacggagg aactcagcca accggtgcgc ggccaacctc gactcctcct
      841 cgcattcgct gcgtcgcact gggtcaagtt catcaccgcg gcatgcacat gagttgatca
      901 gcacgcgccc ctgatcgcga cgcgaggcga acatgcccat ggcccacagg tcgcccacgt
      961 gttctccgaa gcgatccggc agttcaccgc gctcgaccgc ctcgcggacc accgagatcg
     1021 ccatgccgcc gtagtggggc agatggaata ccccgaactc gcgccagttc cggagcgtgt
     1081 cctcgagcga ggtcgggacg tccttctcgg ccgcgtagca gtcgggattg cgctcgagcc
     1141 agtccatgat gccgtcgata tcgaccccgc ccatgcggaa ctggagagtg gagaaccctt
     1201 cgagcacatc gaaagggccg tcactgaggg cgaacagatc caggtccccc gtggcatcca
     1261 cgaactgccg ggcccggaag gcgacaggcc cccccttccc ctcacagacg accgcatcga
     1321 tctcggagcc ggtacccgtc acccgcgata cgaacagccc gtagcgcgat cgcacaccgg
     1381 cctcccggag catctcgatg agtatccact tgagggtctc cgggtcgtag gggatctgtg
     1441 gctgctcggg ccgcgcatag tggggcatcg cgcctcccgc ggccacgagc cgatccagaa
     1501 tccgcgtggc gaggccgcgc acggcacggt tgcccgactg ggtcacgaag aagttcgtgc
     1561 agttgcagac cagtccggcc gtggcgacac cgccgggata gtggcagcgc tcgaccagga
     1621 gtgtatccgc gcccgccctg gccgcg
//
LOCUS       DAOSPL010001235         1643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_74541_length_1643_cov_3.459698, whole genome shotgun sequence.
ACCESSION   DAOSPL010001235 DAOSPL010000000
VERSION     DAOSPL010001235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74541_length_1643_cov_3.459698"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="PLQ54_18565"
     CDS             <1..1314
                     /locus_tag="PLQ54_18565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOS95321.1"
                     /translation="SITDAGALEIHVDRVGRETTLGRIITMVEEAQERKAPVQRLADR
                     FATYFTPAVLAAAAGAYLWTDDVLAAVSVIVVACPCAVALATPLAVVAAVGNAARRGI
                     LVKGGAHLEALARADAFVFDKTGTLTTGRPEVLAVASFDGHTEREILSAAAMVERFSE
                     HPLARAIRRRATECGLPPIEPSGFTPVPGRGVIATDPVSGSMIVVGTADLLRDHGAEL
                     SPDAERRVQTEEARGASVLLVAHDGVPCGYVAVRDQLRPEAASALASLTRLGIRRLVM
                     MTGDGNGAAQAVARELPLSFSHSNLLPGDKTALVRELGQTHRVAMVGDGINDAPALAE
                     AHVGIAMGAAGTDAAIEAADVALMTDDLRRIPEAVALGRQAVATIRRNLVIALAFNIA
                     GVALAASGVLPPAAAAATHVLPDVGVFLNSARLLYWAGLPEAEDN"
     gene            complement(1393..>1643)
                     /locus_tag="PLQ54_18570"
     CDS             complement(1393..>1643)
                     /locus_tag="PLQ54_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95322.1"
                     /translation="PWLFDLSEWDHRRDEATQTEQPWRSDRAGGDGPDPGAEADPEQP
                     GAEHQAEAELAPVQHRQQLAGEYSLDDDRGDPKAHEGE"
BASE COUNT          215 a          619 c          545 g          264 t
ORIGIN      
        1 agtatcacgg atgccggtgc tctcgaaatc cacgtcgacc gcgtcgggcg cgaaacaact
       61 ctgggccgca tcatcaccat ggtcgaggag gcccaggagc ggaaggcccc cgtgcagcgc
      121 ctggccgacc gcttcgccac ctacttcacg cccgccgtcc tcgcggccgc cgccggggcg
      181 tatctgtgga ccgacgatgt gctcgcagcc gtgtcggtga tcgtcgtggc ttgtccgtgc
      241 gccgtggcct tggccacgcc tctggccgtg gtggctgccg taggcaacgc agctcgccgc
      301 gggatcctcg tcaaaggcgg ggcccacctc gaggccctgg cccgggccga tgccttcgtg
      361 ttcgacaaga ccggcaccct gacgacgggc cggcccgagg tcctggccgt cgcctcgttc
      421 gacggccaca ccgagcgcga gatcctctcg gccgcagcga tggtcgagcg cttctccgag
      481 caccccctcg cgcgagccat ccgccgccga gccaccgagt gcgggcttcc gccgatcgag
      541 ccctcaggct tcactcccgt gcccggacgg ggcgtgatcg cgaccgatcc ggtcagcggc
      601 tcgatgatcg tggtcggcac cgccgacctg ctgcgagacc acggcgccga gctctccccc
      661 gatgccgaac gccgggtcca gaccgaggaa gcaagaggag ccagcgtgct gctggtggca
      721 cacgacggag tcccctgcgg ctatgtcgcc gtgcgcgacc agctcaggcc cgaggccgct
      781 tcggcactcg cttccctgac ccggctgggc atccgccgcc tggtgatgat gaccggcgac
      841 ggcaacggcg ccgcccaggc agtggcccgc gagctccctc tgtcgttcag ccactcgaat
      901 ctgctcccgg gagacaagac ggctctggtc cgcgagttgg gccagacgca ccgcgtggcc
      961 atggttggcg acggcatcaa cgacgcaccc gcgcttgccg aggcccatgt gggcatcgcc
     1021 atgggcgcgg ccgggaccga cgccgccatc gaggcggccg atgtcgccct catgaccgac
     1081 gatctccgcc gcattccgga ggccgtggcg ctgggccggc aggcggtcgc gaccatccgc
     1141 cgcaacctgg tcatcgccct ggccttcaac atcgctggcg tcgccctcgc cgcctccggc
     1201 gtgctcccgc cagccgccgc ggccgcgaca catgtcctgc ccgatgtcgg tgtgttcctc
     1261 aactcggccc gcctgctcta ctgggccggc ctgccagagg ccgaggacaa ctgatcggcc
     1321 cccgcgaatg catcccgtgc catggaagcg ccgagcagcc tgagctcgca ccgggtaccc
     1381 gaacggacac tcctactctc cttcatgggc tttggggtct cctcggtcat cgtccaggct
     1441 gtactcgccc gcgagctgct gtcggtgctg cacgggagcg agctctgcct cggcctggtg
     1501 ttcggcgcct ggctgctctg gatcggcctc ggcgcccgga tcggggccgt cgccgcccgc
     1561 tctgtcgctg cgccatggtt gttcggtctg ggtggcctcg tcgcggcggt ggtcccattc
     1621 gctcaggtcg aacaaccatg gcg
//
LOCUS       DAOSPL010001236         1640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_74747_length_1640_cov_2.886435, whole genome shotgun sequence.
ACCESSION   DAOSPL010001236 DAOSPL010000000
VERSION     DAOSPL010001236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74747_length_1640_cov_2.886435"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLQ54_18575"
     CDS             <1..239
                     /locus_tag="PLQ54_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95323.1"
                     /translation="GKTDTVSMSTGPGSITEPSPLSGLSDVEMDLIIDDNYHIQAGRA
                     LAVPSLGESAGVVQWLESVPTNAPYAATARVRLP"
     gene            complement(284..856)
                     /locus_tag="PLQ54_18580"
     CDS             complement(284..856)
                     /locus_tag="PLQ54_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HOS95324.1"
                     /translation="MRDVIRSSFTESIALRERTLEELAGAIEEAAVGSITRVRNGGTV
                     FVCGNGGSAADAQHIAGELAGRFRRERDPLPSICLSANCSTLTAISNDYSFEEVFSRP
                     LAGLARPGDVLWAMSTSGASPNVLLALQVAKQRDLYRVGFSGAGGGPMASLCDVCLAV
                     PSRDTARIQEVHVTIAHILCELIEAQCCGG"
     gene            complement(853..>1640)
                     /locus_tag="PLQ54_18585"
     CDS             complement(853..>1640)
                     /locus_tag="PLQ54_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOS95325.1"
                     /translation="REEPEQVRAMAEALRGTACPARRVDEARARRQAAQADVERQLGS
                     RAATLRAELDVARRYAPFRENPKHYFLMEYELIRAGLLELGKRCGIDDGVFYLVPDEL
                     PRLAAGEDLGDRIRQRRRRRRLALQVPVPEVIFSDDLEAIGRPQATAAAEGALSGLGV
                     SSGSVTGVARVLGHPGEGAQFREGEILVAPSTDPGWTPIVVRAAGLVLEKGGMLSHGA
                     VVAREYGLPAVTNVHEATTVIRTGSRLRIDGTAGRVEVLEGPA"
BASE COUNT          270 a          584 c          557 g          229 t
ORIGIN      
        1 cgggcaagac ggacacggtc agcatgagca cggggcccgg ctcgatcacc gagccgtcgc
       61 ctctctcggg gctgagcgat gtcgagatgg atctgatcat cgacgacaac taccacatcc
      121 aggccggtcg tgcactagcg gtaccatcgc tgggggaatc ggccggcgtg gtgcagtggc
      181 tcgagagcgt gcccaccaac gcaccgtacg ccgcgacggc acgggtcagg ctcccgtagt
      241 gcccttggcc tacagcctca cgacggcgac cgccggcacg cccctatccc ccgcagcact
      301 gcgcctcgat gagttcacac aggatatgcg cgatggtgac gtggacctcc tggatgcgcg
      361 cggtgtcgcg gctgggcaca gcgaggcaca catcgcacag tgaggccatg gggccaccgc
      421 cagccccact gaacccaacc cggtagaggt cccgctgctt ggccacctgg agcgcgagga
      481 gaacgttggg ggaggcgccg gaggtggaca tggcccagag cacgtcaccc gggcgtgcca
      541 ggccggccag cggccggctg aagacctctt cgaacgagta gtcgttgctg atggccgtaa
      601 gggtgctgca gttggcgctg aggcagatcg aaggcagcgg gtcccgctcg cggcggaacc
      661 ggccggccaa ctcacccgca atgtgctggg cgtccgccgc gctaccgcca ttgccgcaca
      721 cgaacacggt gccgccgttg cgcacccgcg tgatggagcc cacggcagcc tcctcgatgg
      781 cgccggcaag ctcctcgagc gtgcgctctc gcagggcgat gctttcggtg aatgaggagc
      841 ggatgacatc tctcatgcgg gtccctccag aacctcgaca cggcccgcgg tcccgtcgat
      901 gcggagccgg gaacccgtgc ggataaccgt cgtggcctca tgcacgttgg tgacagccgg
      961 gaggccgtac tcgcgggcga cgaccgcccc atgcgagagc atgccgccct tctcaagcac
     1021 aagccccgcg gcccggacga cgatgggcgt ccagccgggg tctgtcgagg gcgccacgag
     1081 gatctcgccc tcacggaact gcgcgccctc gcccgggtgg ccgagcacgc gagcgacacc
     1141 cgtcacactc ccgctggaca cccccaagcc tgacagcgcc ccttcggcgg cagcagtggc
     1201 ctgcggccgg ccgatggcct ccaggtcatc gctgaagatc acctcgggca cgggcacctg
     1261 cagggccagc cggcgccgcc ggcgcctctg ccggatccgg tcgcccagat cctcgccggc
     1321 cgcgaggcgc ggtagttcgt cgggaaccaa gtagaacacg ccgtcgtcga taccgcaacg
     1381 cttacccaac tcgagcaatc cggctcggat cagctcgtac tccatcagga agtagtgctt
     1441 ggggttctca cggaacgggg catagcgccg ggccacatcg agctcggcgc ggagtgtggc
     1501 ggcgcgcgac ccgagttgcc gctccacatc ggcctgagcg gcttggcgcc gggcgcgggc
     1561 ctcatcgacg cgtcgagcgg gacaagcggt tccgcgcagt gcctcggcca tggcccgcac
     1621 ctgttcgggc tcctcccgcc
//
LOCUS       DAOSPL010001237         1639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_74795_length_1639_cov_3.805556, whole genome shotgun sequence.
ACCESSION   DAOSPL010001237 DAOSPL010000000
VERSION     DAOSPL010001237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74795_length_1639_cov_3.805556"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="PLQ54_18590"
     CDS             <1..731
                     /locus_tag="PLQ54_18590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132965.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOS95326.1"
                     /translation="AYRQLSLLLRRPPGREAFPGDIFNLHSRLLERAAKLSDALGGGS
                     LTALPIIETQAGDYSAYIPTNVISITDGQIYLEPDLFYKGVRPAINVGISVSRVGGDA
                     QTKMVRSVAKSLRLDLAQYRELEAFAQFASDLDAVTRAQLARGERLVEVLKQPQYRPL
                     SAENQTLQIFAGVRGILDAIPVEDVQRFCAEMVGWVAERHPEILRTLKETQAVSPELE
                     GQIDKALREFAALFAKGSAGSQSQ"
     gene            765..>1639
                     /gene="atpG"
                     /locus_tag="PLQ54_18595"
     CDS             765..>1639
                     /gene="atpG"
                     /locus_tag="PLQ54_18595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOS95327.1"
                     /translation="MLSTRAIRRKIRTVRNIRKITDAMRMVSAARLQRVQAKVSQARP
                     YADKMAELLRHVAPHAREVSHPLLEVRQPARRVALVAVSADRGLCGSFNSNIIRWTSR
                     FLAEQTVPVELITIGRKVGSFFARRNVPIREQFTGISADSTPAEIGVVSSLLRSIYED
                     ETVDEVHILYTEFVNAVVQRPKIMRFLPFEAPAEMGQAEGRYHESEYLFEPSARAVME
                     SLIPRYVDTMIYHLLLESTASEHGARMMAMSNRSTNAGELIDHLTLQLNKARQSSITG
                     ELLEIVAGADALAKQ"
BASE COUNT          285 a          534 c          535 g          285 t
ORIGIN      
        1 aggcctaccg ccagctctcg ctgctgctgc gccgcccgcc ggggcgcgag gccttcccgg
       61 gtgacatctt caacctgcac tcccgcctgc tcgaacgggc cgccaagctc tcagatgcgc
      121 tgggaggcgg ctcgctgacg gcactgccca ttatcgagac gcaggccggc gactattcgg
      181 cgtacatccc caccaacgtc atctcgatca ccgatggcca gatctacctg gaacccgacc
      241 tgttctacaa gggcgttcgg cccgccatca acgtgggcat ctcggtatca cgcgtcggcg
      301 gcgatgctca gacgaagatg gtccggtcgg ttgccaagtc gctgcgcctc gatctggccc
      361 agtaccggga gctcgaagcg ttcgcgcagt tcgcctcgga tctggatgcc gtcacccgcg
      421 cccagctcgc gcgcggcgaa cggctggtcg aagtgctgaa gcaaccccag tacagaccgc
      481 tgtcggccga gaaccagacg ctccagatct tcgcgggcgt gcgggggatt ctggacgcca
      541 ttcctgtcga ggacgtccaa cgcttctgtg ccgaaatggt ggggtgggtc gcggagcggc
      601 accccgagat ccttcgcacc ctcaaggaga cccaggcggt ctcgcccgag ctcgaggggc
      661 agatcgacaa ggcgttacga gagtttgctg ccctgtttgc gaagggcagt gcgggctcgc
      721 agagccagtg aggcctcggc ctcggatgaa gggacgctag ggacgtgctg tccactcgcg
      781 ccatccgccg gaagatccgg acggtccgga acatacggaa gatcaccgac gccatgcgta
      841 tggtgtcggc cgcgcgcctg cagcgtgttc aggccaaggt gagccaggcg cggccgtatg
      901 cagacaagat ggccgaactg cttcgtcacg tggccccaca cgcccgggag gtctcgcacc
      961 cactccttga ggtgcggcag ccagcgcggc gggtggcact cgtggccgtc tccgcggacc
     1021 gggggctgtg cggcagcttc aactcgaaca ttatccgctg gacttcgcgc tttctcgccg
     1081 agcagacggt gccggtggag ctgatcacca tcggccgcaa ggtgggttcg ttcttcgcgc
     1141 gccgtaatgt gccgatccgc gagcagttca cgggcatctc ggcggattcc acgccggcag
     1201 agatcggcgt cgtctcgtcg ctgctccgtt cgatctacga ggacgagacc gtcgacgaag
     1261 tccacatcct ctacacggag ttcgtcaacg ccgtggtgca gcggcccaag atcatgcggt
     1321 tcctgccgtt cgaggcgccg gccgagatgg gtcaggctga aggccggtac cacgagagcg
     1381 agtacctttt cgagcccagc gctcgggcgg tgatggagag cctgatccct cgctatgtcg
     1441 acaccatgat ctaccatctg ctgttggagt cgaccgccag cgagcacggc gctcgcatga
     1501 tggcaatgag taatagatcg accaacgcgg gtgagctgat tgatcacctc accctgcagc
     1561 tcaacaaagc gcgccagtcg tcgatcaccg gggagctcct cgagatcgtt gcgggggctg
     1621 acgcgctggc gaagcagtg
//
LOCUS       DAOSPL010001238         1636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_74971_length_1636_cov_3.275775, whole genome shotgun sequence.
ACCESSION   DAOSPL010001238 DAOSPL010000000
VERSION     DAOSPL010001238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74971_length_1636_cov_3.275775"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLQ54_18600"
     CDS             <1..215
                     /locus_tag="PLQ54_18600"
                     /inference="COORDINATES: protein motif:HMM:NF020483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HOS95328.1"
                     /translation="HRIKEEYGIAQFDVETTRGRTRFGVRSVRENITELETGMVKITD
                     LHGTVYEIPNVNDLDPASRARLYEII"
     gene            complement(654..>1636)
                     /locus_tag="PLQ54_18605"
     CDS             complement(654..>1636)
                     /locus_tag="PLQ54_18605"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOS95329.1"
                     /translation="GPSLRTGRGWHTWDGQGGICLAQFGDERVAILEQYPRSAMEVGY
                     LCPVATLTLEHGRARATSSNFGIPCGVGDGAFGAYVPSVDITDPSAAVVGDRLWVVTC
                     DRSSHPFVSSARWGRFGGDGWELGHELGDGYCPRVAGTPAAGVFVAYIRAKRDQRQGY
                     RSTGRVALQRLADGREWEELPDVPGAHGVGELALAVDPAAGLCIIYTAWGEAGPPLMA
                     IRSADFGRTWGEPRRLTGPETSASSPQVVSHGGRLYVAYVATPRAPGAPPGGVYTFVL
                     DPLQLPGGAGGMDMPFPGRLAGPELFAAASGTAAEASAASPTAPSVQQHR"
BASE COUNT          259 a          581 c          568 g          228 t
ORIGIN      
        1 ttcaccgaat caaggaggag tacggcatcg cccagttcga cgtggagacc acgcgcggcc
       61 gcaccaggtt cggcgtccgc agcgtgcggg agaacatcac tgagctcgag acgggcatgg
      121 tcaagatcac cgacttgcac ggcactgtct acgagatacc caacgtgaat gacctggatc
      181 cggccagccg ggcgaggctg tacgagatca tctagcgatg ggcccgcgac ggaacctcgg
      241 gggtcacgta tcgcggacca gtggtatgtc tcagatcgcg ggaccgagta cgggcccgtg
      301 gcactcgacg atctccggca gtgggtatcc gaaggacgcc tgagccccga ggccgtcgcg
      361 cggagcgacg cgagcgccga ctggcgcccc gtggccgagg tgacggggct aatgccgcca
      421 gggcagggcg cgcctggctt cggctatcag cagttcgcgc agacggttgg gctcgtgcct
      481 gatgccagcc gcgcgcacca tcgcatgcag gcgaagtggg ttggcacagg ctccggggtg
      541 ggtctggtcg ccggcgccat cgccggcttg acgcggggct cctccaccga cctgctgata
      601 tggtctctgg cgggcctcgg cgccggcctg ctgatcggcg cccagcggca tcgtcatctg
      661 tgttgctgga ctgaaggggc ggtaggcgag gcggctgacg cctccgccgc cgtaccgctc
      721 gcggccgcga acagctcagg tccggccaga cgccccggga acggcatgtc catgccgccc
      781 gccccgcccg gcaactgcag aggatccagc acgaatgtgt acacaccacc aggcggggcc
      841 ccaggggccc tgggggtcgc cacgtaggcg acgtagaggc ggccgccgtg ggacacgacc
      901 tggggcgagg acgcgctcgt ctcaggtccg gtcagtctcc tgggctcacc ccacgtgcgc
      961 ccgaagtcgg cagatctgat cgccatcagc ggcggcccgg cctcgcccca tgccgtgtag
     1021 atgatgcaca gccccgcggc tgggtcgacg gccagcgcca gctcaccgac tccgtgggcc
     1081 ccggggacat cgggcagctc ctcccactcc cggccgtcgg ccaagcgctg gagggcgacc
     1141 cggcccgtcg agcgatagcc ctggcgttgg tcgcgcttgg cccgtatgta cgcgacgaac
     1201 acgcccgcgg ccggcgtgcc ggcaacgcga gggcagtagc catcgcccag ctcgtgcccc
     1261 agctcccagc cgtcgccgcc gaaccgcccc catctcgccg agctcacgaa cggatgagag
     1321 gatcggtcgc aggtgaccac ccacagcctg tctccgacca ctgcggcgga cgggtccgtg
     1381 atatccaccg acgggacata ggcgccgaac gcaccgtcgc ccacaccgca ggggatgccg
     1441 aagttgctgg aggtcgcgcg tgctctcccg tgctcgagcg tcagcgtagc cacgggacag
     1501 aggtacccga cttccatcgc ggaccgcggg tactgctcga ggatcgccac gcgctcgtct
     1561 ccgaactgcg ccaggcagat gccgccctgt ccgtcccagg tgtgccaccc gcggccggtc
     1621 ctgagcgatg gcccgg
//
LOCUS       DAOSPL010001239         1636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_74983_length_1636_cov_2.808349, whole genome shotgun sequence.
ACCESSION   DAOSPL010001239 DAOSPL010000000
VERSION     DAOSPL010001239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74983_length_1636_cov_2.808349"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1528
                     /locus_tag="PLQ54_18610"
     CDS             <1..1528
                     /locus_tag="PLQ54_18610"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS95330.1"
                     /translation="GRWSERMEVWRAQSAIRDIRQDLHGSLQRLSIGFFDRTPSGQTI
                     ALATSDVDQLRRFLGFAVFELTRGLLSFIGVLVICIQLHPGLAALSLAPAPVMVWVSA
                     RYAHRARPLFADIRTRVAEMTARLQEGLAGTRVVRCYLQEEREVARFLHSAQRLREGH
                     VEAARLMSFYLPLLQLFSALGTGVVLAYGGTLAMQGKLSVGSLVAFMAYQARLNWPVR
                     MAGFAVSLAEQAITAGQRVFGIIDQPSEVEDARDAVDLDTVRGEVEFDDVSFSYDGRL
                     PALEHVSFRVPAGSRVALLGPTGSGKSTLMALLMRFYDPTGGRVLVDGVDVRNVRQAS
                     LRSHMAAVLQDSMLFGDTIRGNIAFGRPNAGDEEIARAAELAQVSGFVGELEKGFDTE
                     LGERGAGLSGGQRQRVALARALVTDPAILILDDCTSSVDAATEQAIQAGLDEAMAGRT
                     TFVIGQRPSTLRQADLILVFDGGRLVEMGTHDELAARSGLYREVLEASGTTADGRGAP
                     "
BASE COUNT          222 a          540 c          592 g          282 t
ORIGIN      
        1 gggccgctgg tcggagagga tggaggtctg gcgcgcccag agcgccatcc gcgacatccg
       61 gcaggacctg cacggcagcc tgcagcggct ctccatcggc ttcttcgacc gtaccccgtc
      121 aggccagacc atcgcgctcg ccaccagtga tgtggatcag ctccggcgat tcctcgggtt
      181 cgccgtgttc gagctgaccc gggggctgct gagcttcatc ggtgtgctcg tcatctgcat
      241 ccaactccac ccgggcctgg cggcgctatc cttggcgccg gctccggtca tggtctgggt
      301 ctccgcccgc tacgcccatc gagcccggcc gctgttcgcg gacatccgca ccagggtcgc
      361 cgagatgacc gcccggttgc aggagggcct ggccggcaca cgcgttgtgc ggtgctatct
      421 ccaggaggaa cgcgaggtgg cgcgcttcct ccattcggcc cagaggttgc gcgaaggcca
      481 tgtcgaggcc gcccggctca tgagcttcta cctccccctg ctccagctct tctcggcgct
      541 gggaacgggc gtcgtcctgg cctacggcgg caccctggcc atgcagggca agctgtcggt
      601 cggctcgctg gtggctttca tggcctatca agcccggctc aactggccgg tgcgaatggc
      661 ggggttcgcc gtctccctgg ccgagcaggc cattacggcg ggccagcggg tgttcggcat
      721 catcgatcag ccgtctgagg tcgaggacgc acgcgacgcg gtagatctcg acaccgtccg
      781 gggcgaggtc gagttcgacg acgtcagctt ctcctacgac ggtcgcctcc cggccctcga
      841 gcacgtgagc ttccgggtgc cggcggggtc gcgtgtggca ttgctcgggc caacgggaag
      901 cggcaagtcg acactgatgg cgctgctgat gcgtttctac gacccgacgg gtgggcgggt
      961 gctggtcgac ggtgtcgatg tccggaacgt gcgacaggcg tcgctccggt cgcacatggc
     1021 ggcggtgttg caggattcga tgctcttcgg cgacaccatc cgcgggaaca tcgcattcgg
     1081 gcgccccaat gccggcgacg aagagattgc ccgggccgcc gagttggccc aggtgtcggg
     1141 cttcgtcggt gagcttgaga aggggttcga caccgagctg ggggagcgcg gcgccgggct
     1201 tagcggcggc cagaggcagc gggtagccct ggctcgcgcc ctggtgaccg atcccgcgat
     1261 cctgatcctc gacgactgca cgtcgagcgt ggacgcggcg accgagcagg cgatccaggc
     1321 aggcctggat gaggccatgg ccgggcgcac cacgttcgtg atcggacagc ggccctctac
     1381 tctccgccag gccgacctga tcctggtgtt cgacggcggg cggctcgtgg agatggggac
     1441 ccacgacgag ttggcggcga gatcggggct ctaccgcgag gtcctggagg cctccggtac
     1501 gacagcggac ggtcgtggtg ccccatgatg cgcggggccg gtttgcggat gggcggcggc
     1561 ttcggtcggc cgaggcccga ggaggacgtg cggatcctca acccgcgcct catggcgcgg
     1621 ctcctgctcc tcatgg
//
LOCUS       DAOSPL010001240         1636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_75002_length_1636_cov_2.328273, whole genome shotgun sequence.
ACCESSION   DAOSPL010001240 DAOSPL010000000
VERSION     DAOSPL010001240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75002_length_1636_cov_2.328273"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="PLQ54_18615"
     CDS             complement(<1..1311)
                     /locus_tag="PLQ54_18615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS95331.1"
                     /translation="MTDLPRLEGSVLIADDEEAICRGIKRVLTSRGAEVDMAHSGADA
                     LKRLTSTAFDVALVDLVMPSFGGFELLERIEQAELKVVAIVITAHASIDTAVEAIRKG
                     AFDYLPKPFAPEDLVLRVHRALAWRHLRREAETRLLELNSDKTQLRTILNSLADGVIV
                     VNVDGRVVLTNPAVRAALGLGPSPHDPQPLESVVADPALAELIRRAPEVGPGGRASLS
                     THVEVGQHVYMARVVPIEDSRKAYLGSATVLRDVTELMSLERAKSRFMSMVAHELKSP
                     LAAVQGYLNVVLGEAPPDDGRREEVLTRCRERVEGMAELVRDLLELSRSEAPAVRRTE
                     ELAVADIVTQATNVSHDLAERHEVSLEVQVAPDCPPLHADRAEVVRILTNLVSNAIKY
                     NRPGGRVSIRAVPDSQGCRLDVEDDGLGIPEDALPRLGEEFFRVN"
     gene            complement(1304..>1636)
                     /locus_tag="PLQ54_18620"
     CDS             complement(1304..>1636)
                     /locus_tag="PLQ54_18620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95332.1"
                     /translation="VANREIPDAFVLDLIMERTDSGARLARALRRDPRFRDAPIVILT
                     SVVHDVGFEFHRNPGEVLAWMKADAWFDKPVPVQTLVSTIRRIHAERLAAREPRVAEN
                     AEQVAAHD"
BASE COUNT          264 a          533 c          561 g          278 t
ORIGIN      
        1 gttcacacgg aagaactcct cacccaggcg cggcagagcg tcctcgggga ttccgagacc
       61 gtcgtcttcg acatcgaggc ggcatccctg gctgtcgggc acggcccgaa tgctgacccg
      121 gccacccggg cgattgtact tgatcgcgtt actcaccaga ttggtaagga tgcgcacgac
      181 ttcggcgcgg tcggcatgga gtggggggca atcgggcgcc acctggactt cgagggagac
      241 ctcgtgtcgt tcggccaaat cgtggctcac gttggtggcc tgcgtcacga tgtcggccac
      301 agcgagttcc tcggtccggc gcaccgcagg agcttccgaa cgcgagagct ccagaaggtc
      361 gcgcaccagt tcggccatgc cctccactcg ctcgcggcag cgcgtgagga cctcttcgcg
      421 cctgccgtcg tccggcggcg cctcgcccag aacgacgttc aggtaacctt gtaccgcggc
      481 caggggcgac ttgagctcgt gagcgaccat cgacatgaac cgcgacttgg cccgttcgag
      541 gctcatcagc tcagtgacgt cgcggagcac cgtggcactc ccgaggtagg ctttgcggga
      601 gtcctcgatg ggcacgaccc gcgccatgta gacgtgctgg cccacctcga catgggtact
      661 gaggcttgcg cgcccgcccg gccctacttc gggcgcccgg cggatcagtt ctgccaacgc
      721 gggatcggcg accacggact ccaggggctg tgggtcgtgc gggctcggcc ccagcccgag
      781 ggccgcgcgg acagcgggat tcgtcagcac gacgcggccg tccacgttca ccacgatgac
      841 gccatcggcc aggctgttga gaatggttcg gagctgggtc ttgtcggagt tgagctccag
      901 tagccgcgtc tccgcctctc tgcgcaagtg acgccaggcc agcgcacggt ggactctcag
      961 gaccaagtcc tccggagcga agggtttggg gaggtagtcg aaggcgccct tgcgtatggc
     1021 ctcgacggcc gtgtcgatgc tggcatgtgc ggtgatgacg atggcgacga ctttgagctc
     1081 agcctgctcg atccgctcga ggagctcgaa cccgccgaag ctgggcatca ccaggtcgac
     1141 cagggccacg tcgaacgcgg tggaggtcag ccgcttcagc gcatcggcgc ccgagtgggc
     1201 catatcgacc tcggccccgc ggctggtcag gacgcgcttg atgccgcggc agatcgcctc
     1261 ttcatcgtcg gcgatcagca cggaaccttc gagccgcggg agatcagtca tgggccgcca
     1321 cctgctcagc gttctcggcg actcgcggct cgcgcgcggc cagacgctcg gcatggattc
     1381 ggcgaatggt gctgaccagt gtctggaccg ggacgggctt gtcgaaccat gcatcggcct
     1441 tcatccaggc cagcacttcg ccgggattgc ggtgaaactc gaagcccaca tcgtgcacga
     1501 ccgatgtgag gatgacgatg ggggcatcgc ggaaccgggg gtcgcgccgc agcgctcggg
     1561 cgagtcgcgc gcccgagtcg gtccgttcca tgatgaggtc gaggacaaat gcatcgggga
     1621 tctcccggtt ggccac
//
LOCUS       DAOSPL010001241         1634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_75097_length_1634_cov_3.153895, whole genome shotgun sequence.
ACCESSION   DAOSPL010001241 DAOSPL010000000
VERSION     DAOSPL010001241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75097_length_1634_cov_3.153895"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="PLQ54_18625"
     CDS             <1..1150
                     /locus_tag="PLQ54_18625"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOS95333.1"
                     /translation="MDVWLPLPADDASQSIVAAQVTPRPHEVETTRHGQRVARWQGIR
                     LAGGACFTVRLVIDATLHPSALPAGRELAEAERRLYLADGPKYRLGSLPVRSAAAEIA
                     PGARGSQAMVGGVFDYLVDHLTYSRDGTWDPADAVLERGTGSCSEYVFAFIALCRAKG
                     IPARYVGGSARRTGSSLHVDRVWHRWAEAFVDGIGWQPFDPTRSDGPERFRRHFGSPP
                     RDVLALVRGDGADGPPLGWRYDSYHEWDGNRSDIRAQRRGWWIPVPSARVREQVTALG
                     PGVSVARAREIAHPFVLPWLDELLYDRGARIEAAKTMRDIGGPSVAPALIDSLERAHD
                     PSGDEAIATLLNEWTGQGLGATWGDWDAWTRTQSFRDFTAQGRAGEER"
     gene            1196..>1634
                     /locus_tag="PLQ54_18630"
     CDS             1196..>1634
                     /locus_tag="PLQ54_18630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95334.1"
                     /translation="MSIQVAAAGAMLLVGTILPAGNRNLAGPTETAMIALEVGMTTPE
                     EPRQQASEAEERVIVPPSDPIGPMWDLGRFGQAPMYEEATGYQWAETSEGVRPIFFDG
                     PEYRGRPTRVFAWLGVPDAPPGTRLPGMVLVPGGGGTAFAHWVK"
BASE COUNT          228 a          536 c          607 g          263 t
ORIGIN      
        1 gatggacgta tggcttccgc tccccgcgga tgacgccagt cagtccatcg tggcggctca
       61 ggtgacaccg cggccccacg aggtcgagac gacccgccac ggacagcgcg tagctcgatg
      121 gcagggcatc cgcttggccg ggggcgcctg tttcactgtc cggctcgtca tcgacgcgac
      181 gctgcatcca tccgcgctgc cggccggccg cgagctggcc gaggcagagc gccgtttgta
      241 cctggccgac ggcccaaagt accggctcgg ctctctgccg gtccggtccg cggcggcgga
      301 gattgcgccc ggcgcccgcg gcagtcaggc catggtgggc ggtgttttcg actacctcgt
      361 tgatcacctc acctactccc gggacggcac ctgggatccg gccgacgcgg tcctcgagcg
      421 tggcacgggc agctgctccg aatacgtctt cgccttcatc gcgctctgtc gcgccaaggg
      481 gatcccggcc cgctatgtgg gcggatcggc gcgccgaacc ggctcgtcgc tccatgtgga
      541 ccgcgtatgg caccgctggg ccgaggcctt cgtcgacgga atcggctggc agccatttga
      601 ccccacgcgc agcgacgggc cggagcgctt ccggcgtcac ttcggcagcc cgccgcgaga
      661 cgtcctggct ctggttcgcg gcgatggcgc agacggcccg cccctgggct ggcggtacga
      721 ttcgtaccac gaatgggatg gcaaccggtc ggatatcaga gcccagcggc gtggatggtg
      781 gatcccggtg ccgtcggccc gggtgcgcga gcaggtgacg gcgctggggc ccggtgtgtc
      841 ggttgcccgg gcccgcgaga tcgcccaccc gttcgtgctg ccgtggctcg acgagctcct
      901 gtacgatcgc ggggcacgga tcgaggcggc caagacgatg cgcgatatcg gtgggccgtc
      961 cgtcgcgccg gcgctcattg atagcctcga gcgcgcccat gacccctcgg gcgacgaggc
     1021 gatcgccacc ctcctgaacg agtggacggg tcaggggctc ggcgccacgt ggggcgactg
     1081 ggacgcctgg acgcggacgc agtcgttccg cgatttcacg gcccagggac gagcgggaga
     1141 agagcgatga gaggcagccc cgcggcgggg cgtgttggtt cgagggggtt gtcctatgag
     1201 tatccaggtg gcagcggctg gggccatgct tctggtgggt acgatcctcc ccgcaggcaa
     1261 caggaacctg gccgggccga ccgagacggc catgatcgcg ctggaggtgg gtatgacgac
     1321 gccggaggag ccgcgtcagc aggccagtga agccgaggag cgagtgatcg tgcctccctc
     1381 cgatcccatc ggcccgatgt gggacttggg gcgcttcggc caagcgccga tgtatgagga
     1441 agccacaggc taccagtggg ccgagacgtc cgagggcgtg cgtccgatct ttttcgatgg
     1501 tcccgagtac cgcggccggc cgacgagggt gttcgcgtgg ctcggtgttc ccgatgcgcc
     1561 gccggggacc aggttgcccg gcatggtgtt ggtccccggc ggcggaggca ctgcgttcgc
     1621 tcactgggtg aaga
//
LOCUS       DAOSPL010001242         1634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_75101_length_1634_cov_3.093097, whole genome shotgun sequence.
ACCESSION   DAOSPL010001242 DAOSPL010000000
VERSION     DAOSPL010001242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75101_length_1634_cov_3.093097"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1161)
                     /locus_tag="PLQ54_18635"
     CDS             complement(79..1161)
                     /locus_tag="PLQ54_18635"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOS95335.1"
                     /translation="MASVDSRLELLRAVTETDGVVGHEDAVRALMADRLSAVGEISYD
                     RQGCIFCTKAGTSASPRVMLAAHMDEIGFLVKLVTDEGLLRFTPVGGWFDQVLLAQRV
                     RVTTRKGSYVGVIGSKPPHLLHDEDRRKVIQQRTMFIDIGATSKAQAEEEFGVRPGDP
                     IAPVSEFCALGDGSRLMGKAWDDRVGVALAIEALLEAGPDHPNTLIAAGTVAEEMGLR
                     GATTAPWTAEPDVAIVLESAIAGDVPGTKPEESSEKLGAGPSILFREGAALPSRAFRE
                     FALSVASEEGIPVQHGFLEGGSTDGRVIALHRVGVPVLVLGVAARYIHTHAQIIARQD
                     YENALKLLVAMVRRLDAARVESFTAW"
     gene            complement(1213..>1634)
                     /locus_tag="PLQ54_18640"
     CDS             complement(1213..>1634)
                     /locus_tag="PLQ54_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOS95336.1"
                     /translation="TTREIAKVLELLQVNSNSCTASAVQMAGVAALRGPQDCVDRMVA
                     EFDRRRRFIVEGVNNLPGWSCRMPRGAFYLFCNVKQLGMEVVELRDRLLDEAGVATMH
                     GTDFGVFGEGYMRVSYATSIEQLEEGLRRIAEWCERL"
BASE COUNT          274 a          600 c          514 g          246 t
ORIGIN      
        1 cgatgccgac cgccagggcg gccgcgatgc accgagtcgg acgcccagag ctcgttcggc
       61 ccccacgggc cctgcgctct accatgccgt gaacgactcc actcgggccg catccagccg
      121 ccgcaccatg gcgaccagca gctttagcgc gttctcatag tcctgccggg cgatgatctg
      181 ggcgtgcgta tgaatgtagc gtgccgccac gcccagtacg agaaccggca cgcccacgcg
      241 atgaagggca atgacccggc cgtcggtcga gcccccctcg aggaaaccat gctgcaccgg
      301 gatgccctcc tcggaggcca cactcagagc gaactcgcgg aacgcccgcg atggcagcgc
      361 ggccccctca cggaacagga tcgacgggcc ggcgccgagc ttctcggacg actcctcagg
      421 cttcgtaccg ggcacgtcgc ccgcgatggc gctctcgagg acgatggcga cgtcgggctc
      481 ggccgtccac ggtgccgtgg tggcgccgcg aagccccatc tcctcggcca cggttcccgc
      541 cgcgatcagg gtgttggggt gatcgggacc ggcctcgaga agcgcctcga tcgccagcgc
      601 cacgcccacg cgatcgtccc acgccttccc catgagccgt gatccgtcgc ccagggcaca
      661 gaactcgctc accggggcga tggggtcgcc cgggcggacg ccgaactcct cctccgcctg
      721 cgccttggaa gtcgcgccga tatcgatgaa catggtgcgt tgctggatca ccttgcgccg
      781 atcctcatca tgcagcaggt gcggcggctt ggagccgatg acccccacgt aggagccctt
      841 gcgtgtcgtg acacggaccc gctgggccaa gagcacctgg tcgaaccagc cgcccaccgg
      901 ggtgaagcgc aggagtccct cgtcggtcac cagcttcacg aggaacccga tctcgtccat
      961 atgagcggca agcatcacac ggggcgacgc cgaagtgccg gccttggtgc agaagatgca
     1021 tccctggcgg tcgtagctga tctcgccgac ggccgagagc cggtcggcca tgagcgcccg
     1081 caccgcgtcc tcgtggccaa ccaccccgtc cgtctccgtc accgcgcgca gaagctcgag
     1141 tcgcgaatcc acagatgcca ccgctctcgt ctcgatcggt cgcgagcccc atcgggctcg
     1201 tgtcgccagc cgctacagcc tctcgcacca ctccgcaata cgccggaggc cctcttcgag
     1261 ttgctcgatc gacgtggcat aggacactcg catgtacccc tcaccgaaga ccccgaagtc
     1321 cgtgccgtgc atcgtggcaa cgccggcctc gtcaagcagc cggtcacgga gttcgacgac
     1381 ctccatgccc agctgcttca cattgcagaa gaggtagaag gccccccggg gcatccggca
     1441 cgaccacccg gggaggttgt tgacgccctc aacgatgaag cggcgccggc ggtcgaactc
     1501 ggcgaccatc cggtccacgc agtcctgagg gccgcgcagc gcggcgactc ccgccatctg
     1561 caccgcgctg gccgtgcatg agttggagtt cacctggagg agttcgagga ccttggcgat
     1621 ctctcgcgtg gtca
//
LOCUS       DAOSPL010001243         1634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_75107_length_1634_cov_2.955035, whole genome shotgun sequence.
ACCESSION   DAOSPL010001243 DAOSPL010000000
VERSION     DAOSPL010001243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75107_length_1634_cov_2.955035"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="PLQ54_18645"
     CDS             <1..1179
                     /locus_tag="PLQ54_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95337.1"
                     /translation="VPLLWLDGTDASLRRLESLPPPPLAPHRASASDLDVMLSGGVGM
                     VSFVMTRDEGPLAVSMLLARVEGRWHIPAVAAGPLDRIVGGPPVIPGDTVLVDGIPPL
                     LGRDADDATAYVGYILNRNLSRTDRERFRAALVRDFAGADEATRHGLLALSEAWRDIA
                     SMPWAERSATRRTELQTALDGARTHPELESARALLALFEQSRDVLVAGPMPLTHEAAT
                     AYAGLMASITSVLATGDIQAPEPQVRQLMAELVRAYHNMPDTEKERVAQAPVTWARLQ
                     ALWQTAGDDEREALTEFLALRLRVTAHQASQGGAYLPKDMAQRLGGDEAVRYRERLFP
                     EPAGGAPQRFNFDEAVNRAYDEGRAEVAAALNETRHRITQELIEKMMPPVRKPPTADP
                     "
     gene            complement(1176..>1634)
                     /locus_tag="PLQ54_18650"
     CDS             complement(1176..>1634)
                     /locus_tag="PLQ54_18650"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOS95338.1"
                     /translation="QVEVELRARYDVVDNLVSVVKAAAEHERSVLEAVTRARGSAESA
                     AGIAAQISAQGELAGHIRSLIALGERYPSLRTHTNFLKLQQQLSAIETKIAYARGFYN
                     ESVTEYNTTVGKFPLLIVAKLTGHAPCPHYVTEEHLARGVAEPPHVAIDR"
BASE COUNT          232 a          553 c          590 g          259 t
ORIGIN      
        1 gtcccgctgc tgtggctcga cgggaccgat gccagcctcc gccggctgga gtctttgcct
       61 ccccctccgt tggcccccca ccgggcgtcg gccagtgacc tggatgtgat gctgtcgggc
      121 ggcgtgggga tggtctcctt tgtgatgacg cgcgatgagg gtcccctggc ggtctcgatg
      181 ctgctggccc gggtcgaggg acgctggcac attcccgccg tggcggccgg accgctcgac
      241 cgcatcgtgg gcggcccccc ggtgatcccg ggcgacaccg tgctggtgga cggcatcccg
      301 cccctgctgg gccgcgatgc cgatgatgcg acggcctatg tgggctacat cctcaaccgc
      361 aacctcagcc gtaccgaccg ggagcgcttc cgagctgctc tggtccgcga ctttgcgggt
      421 gccgatgagg cgacccgcca tggactgctg gctctgtccg aagcctggcg tgacatcgcc
      481 tccatgccct gggcggagcg gagcgcgacg aggcggaccg agctccagac agcgctcgat
      541 ggcgcgcgca cccacccgga gcttgaatcg gctcgggccc tgctggcgtt gttcgagcag
      601 agccgcgacg tgctcgtcgc cggaccgatg cccctcacgc acgaggccgc gaccgcctac
      661 gcgggcttga tggcctcgat cacgtctgtg ctggccacgg gggacattca ggcccccgag
      721 ccccaagtgc gccaactgat ggcggagctg gtgcgggcct accacaacat gcccgacacg
      781 gagaaggagc gagtggctca ggcgccggtc acctgggcgc ggctgcaggc cctgtggcag
      841 acggctgggg acgatgaacg ggaggcgctg accgagttcc tggccctccg gttgcgcgtc
      901 acggcccacc aggcatccca gggcggtgcg tacctgccca aggacatggc ccagcgtctg
      961 ggcggcgatg aggccgtccg gtacagggag cgcctgtttc ccgagcccgc gggcggggcg
     1021 ccccagcgct tcaacttcga tgaggcggtg aatcgggcct acgacgaggg gcgtgccgag
     1081 gtcgcggccg ccctcaacga gacgcgccac cggatcaccc aggagctcat cgagaagatg
     1141 atgccgccgg tgcggaagcc gccgacggcc gatccctagc gatcgatcgc tacgtggggc
     1201 ggctcggcga ccccgcgggc aagatgctcc tcggtcacat agtgcgggca cggggcgtga
     1261 cccgtgagct tggcgacgat gagcagtggg aacttgccga ccgtcgtgtt gtactcggtg
     1321 accgactcat tgtagaaccc gcgggcatag gcgatcttcg tctcgatggc cgagagctgc
     1381 tgctggagct tgaggaagtt cgtgtgcgtc cggaggctcg ggtaccgctc gccgagggcg
     1441 atgaggctgc ggatgtgccc cgcgagctca ccctgggcgc tgatttgggc cgcgatgccg
     1501 gcggcggact cagccgagcc gcgggcccgg gttacggctt cgagcacgga gcgttcgtgc
     1561 tcggccgccg ccttcacgac cgacaccagg ttgtcgacga cgtcgtagcg tgcccggagc
     1621 tccacctcga cctg
//
LOCUS       DAOSPL010001244         1633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_75180_length_1633_cov_2.309252, whole genome shotgun sequence.
ACCESSION   DAOSPL010001244 DAOSPL010000000
VERSION     DAOSPL010001244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75180_length_1633_cov_2.309252"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLQ54_18655"
     CDS             complement(<1..295)
                     /locus_tag="PLQ54_18655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95339.1"
                     /translation="MSRNTTRMFTERAEAALRAARQEAARGETGVVTTGDALIGLAGS
                     GAGTGYELVRAARITPDGARQRMRGAPPAMDAAPGSSGFSRGLELAIERAHREA"
     gene            complement(402..>1633)
                     /locus_tag="PLQ54_18660"
     CDS             complement(402..>1633)
                     /locus_tag="PLQ54_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95340.1"
                     /translation="TLTSSPRRAPLAARRALPPWAAVLVVLAGLSVSASLGEPPATPA
                     SLVAQAQRALRTYDDTEGERLLRLALERDSGLASAHRELGLLLARQYRYEDAERHLSL
                     FLDGRREPDPLALAARARMRFALLQWDGALSDALDVLAVPPSEVDASLVAEMRGLSVA
                     LGDATPRYTIEVRDSAGRLQCVLRAPGDTDQVRWYARSAATALAQVESLAGQELPPVG
                     LILLRDGKAMNRWSRAFTGRKAPGCAFCDHRVIVLELEANRYRRGDRAAEERARMTVL
                     RHELIHHMVDALAGARATPKWLNEGIAEWFCRQFEPAEPAPEAQRNFEWASRVSPEWY
                     FVASQPEQMDERYYAFAALFVRLASERAPAGDGWVRAFLADRGAGLPTDEAFSRSVGA
                     AKEDVFGALVEAARAVR"
BASE COUNT          261 a          600 c          555 g          217 t
ORIGIN      
        1 gagcctcccg atgcgcccgc tcgatggcga gctcgaggcc acgggagaaa ccgctgctcc
       61 ccggcgcggc atccatggcc ggaggagccc cacgcatcct ctggcgcgcc ccgtccggcg
      121 tgatccgtgc cgcccgcact agctcgtacc cggtcccggc ccccgagccg gcgaggccaa
      181 tcagggcgtc gcctgtggtg acgacacctg tctccccgcg agccgcctcc tgtcgggcgg
      241 ccctcaacgc ggcctcggca cgctcggtga acatgcgtgt ggtgttccgc gacacgacag
      301 ccaccctctc gcgcaggctc tgtcccgcag gggctctgga ggaccaggcc agcacggtat
      361 tcgggagccc acaggagtcg gcctgcccga agcgtgcgcc atcaccgcac ggcccgggcg
      421 gcctcgacga gggccccaaa gacgtcctcc ttggccgcac ccaccgaacg cgagaacgct
      481 tcgtcggttg gcagaccggc gccgcggtca gcaaggaacg cgcgcaccca tccgtcaccg
      541 gcgggagccc tttcagacgc gagacggacg aacagagccg cgaatgcata gtagcgctcg
      601 tccatctgct ccggctggct ggccacaaag taccattcgg gactgacgcg cgaggcccat
      661 tcgaagttac gctgggcttc cggggctggt tccgccggct cgaactggcg gcagaaccac
      721 tcggcgatcc cctcgttcag ccacttggga gtcgcccgcg cgcccgcaag ggcgtcgacc
      781 atgtggtgaa tcagctcatg ccgcaggacg gtcatgcgcg cgcgctcctc ggcggcccgg
      841 tcaccgcgcc ggtagcggtt ggcctccagt tcgagcacga tgactcggtg atcacagaac
      901 gcgcagccgg gtgccttccg gccggtgaac gctcggctcc accggttcat cgccttgccg
      961 tcacgcagga ggatgagccc caccggcggg agttcctgtc ccgccagcga ctccacctga
     1021 gccaaggcgg tggcggccga tcgggcgtac cagcgtactt ggtcagtatc cccgggcgcc
     1081 cgcagcacgc actgaagccg tccggccgag tcgcggacct cgatggtgta ccggggcgtg
     1141 gcatccccga gggcgaccga gagtccgcgc atctcggcaa cgagcgacgc gtcgacctcg
     1201 ctgggcggca ctgcgagcac gtccagcgcg tcggacagcg ccccatccca ttgcaggagc
     1261 gcgaacctca tgcgggctcg cgccgccaga gccagaggat ccggctcgcg ccgcccatcg
     1321 aggaacagag agaggtgccg ctcggcgtcc tcgtaccggt actgcctcgc cagcagcagt
     1381 cccaactcgc ggtgggccga ggcgagaccg gaatcacgct cgagggcgag ccgcagcagc
     1441 cgctcgccct cggtgtcatc ataggtgcgc agagcacgtt gggcctgagc gaccaggctg
     1501 gcgggcgtag cggggggctc gcccaggctt gccgacaccg acaggcccgc gagcacgacc
     1561 agcacagcgg cccacggagg tagggctcgg cgcgccgcaa gcggcgcgcg tctaggcgaa
     1621 gacgtcaagg ttc
//
LOCUS       DAOSPL010001245         1632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_75223_length_1632_cov_3.072289, whole genome shotgun sequence.
ACCESSION   DAOSPL010001245 DAOSPL010000000
VERSION     DAOSPL010001245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75223_length_1632_cov_3.072289"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="PLQ54_18665"
     CDS             <1..755
                     /locus_tag="PLQ54_18665"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOS95341.1"
                     /translation="ETSGDLAGLFEQAARYLDNEHKIRSKISLYTFYPKALLVFALLI
                     STVPTLFLGSLGAWLVEVARLAASVGVPILVLWAAFRIALGYPTIAHGYDRVKLAIPW
                     FGGVIRKFCGARFCRCLGYLYHAGVPIVSATDVGAKSCGNLHMRDRLLGLSPLLQQGA
                     PISDVLARSGQFPPEVIHMAAAGEKSGDLDSLLTKVADYMEEEADTSANKAVLLSLPL
                     SVVLIGFIVVLRLAMGMLMGSEYVKTIQQMTQ"
     gene            755..>1632
                     /locus_tag="PLQ54_18670"
     CDS             755..>1632
                     /locus_tag="PLQ54_18670"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOS95342.1"
                     /translation="MPQPTDPRAGSHYDVLGVDPAATPEQIRARYRELARTQHPDVVS
                     AEAKPEANTAFVRILEAYRVLSDPERRRRYDAALGVHAMPGTIDLDDLASAFDVVDEL
                     LIEANTHLACGRLHSAQRCCEDALEREPGNAQAEALLREVRSEMARGHGRREPPPCHG
                     ADAPAPAGRFRVVPLATVRPLEPLRTSRARATLCAAALAGAVAAGRWMLDTGPGATAL
                     GLPARSFASCAVLAGLVAFALDVAALIGPFDEEMASHAPGEGGGLGLAYLPLIVAAAA
                     LHVVLALVVYVAIAAV"
BASE COUNT          220 a          596 c          550 g          266 t
ORIGIN      
        1 gcgagacctc gggcgatctg gcgggcttgt tcgagcaggc ggcccgctac ctcgacaacg
       61 agcacaagat ccgcagcaag atctcgctct acaccttcta ccccaaagca ctgctcgtgt
      121 tcgccctcct gatcagcacg gtgcccacgt tgttcctggg cagcctggga gcttggctcg
      181 tcgaggtcgc gcggctggct gcatcggtcg gggtcccgat cctcgtcctg tgggcagctt
      241 tccgcatcgc cctggggtat cccaccatcg cgcatggcta cgaccgggtg aagctcgcca
      301 tcccctggtt cggtggcgtc atccgcaagt tctgcggcgc ccggttctgc cggtgcctgg
      361 gctacctcta ccatgccggt gtgcccatcg tctcggcgac cgacgtgggc gccaagagct
      421 gcggcaacct gcacatgcgc gatcggctgc tcggcctgag tccgctcctg cagcagggcg
      481 cccccatctc cgatgtcctc gcccgctcgg ggcagttccc ccccgaggtc atccacatgg
      541 cggccgcggg agagaagtcg ggcgacctgg acagtctcct gaccaaggtc gccgactaca
      601 tggaggaaga ggccgacaca tcggccaaca aggccgtgct gctcagcttg cccctgtcgg
      661 tcgtgctcat cggcttcatc gtggtcctcc ggcttgccat ggggatgctc atgggtagcg
      721 agtacgtcaa gaccatccag cagatgaccc agtgatgccg cagcccacgg atccgcgagc
      781 cgggtcgcac tacgatgtgc tgggcgtcga ccccgcggcg acgcccgagc agatccgcgc
      841 gcgctaccgc gagctggcgc ggacgcagca cccggacgtg gtgtctgcag aggccaaacc
      901 cgaggccaac acggcgttcg tccgcatcct cgaggcctac cgcgtcctga gcgaccccga
      961 gagacggcgc cgctatgatg ccgccctcgg cgtccatgcc atgcccggaa ccatcgacct
     1021 cgatgacctg gccagcgcct tcgatgtggt cgatgagctc ctgatcgaag cgaacacgca
     1081 cctcgcctgc gggcgcctgc attccgccca acgctgctgc gaggatgccc tggagcggga
     1141 accagggaat gctcaggccg aggcgctgct gcgcgaggtc cgatccgaga tggcccgggg
     1201 ccatggccgc cgcgagccgc caccctgcca cggggctgat gccccggccc cggccgggcg
     1261 attccgggtg gtcccgctcg ccaccgtgcg gcccctcgag cccttgcgga catcgcgggc
     1321 ccgtgccact ctgtgtgcgg cggcgctcgc gggggccgtg gccgcggggc ggtggatgct
     1381 cgacacgggc cccggcgcga ccgccctcgg gctcccggcg cgcagcttcg ccagctgcgc
     1441 cgtgctggcc ggcctggtgg ccttcgccct cgatgtggcc gcgctgatcg gtcccttcga
     1501 tgaggagatg gccagccacg cgccgggcga gggcggcgga ctcgggctcg cgtacctgcc
     1561 gctcatcgtg gcggcggccg ccctgcacgt ggtgctcgcc ctcgtggtct acgtggccat
     1621 cgccgcggtg tg
//
LOCUS       DAOSPL010001246         1632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_75231_length_1632_cov_2.478757, whole genome shotgun sequence.
ACCESSION   DAOSPL010001246 DAOSPL010000000
VERSION     DAOSPL010001246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75231_length_1632_cov_2.478757"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1632
                     /locus_tag="PLQ54_18675"
     CDS             <1..>1632
                     /locus_tag="PLQ54_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95343.1"
                     /translation="VYVVDGGPRIEPFDYVVVERRFGSYDMRYTHPRVRGIGNPMDSP
                     NWHWRGEPGQVRFSLVPHPQPLPEAMVDPGETCLQVDALVAEEASINQIVFIGTDHGG
                     ESIWYGQLEPGRHYRLSVWLRQEGLANGGAVRFSYGQGYPGISHHFGVTNQWAEYTCE
                     FDGPERPADPWHFGHTFTFTGPGTLWMDNCRIARVDRPEDATKPYVPNATVLDELKAA
                     SPPAGPKGMQRCWVLARDSTMDHLLSWYGNSQVRPDWSTSISGTVDMTMPQLLEFCRL
                     TGPDPTQRVVPWIVIQHILHDEDDWLALVEYLAAPYDPAADSPETKPWAFRRFRQRGT
                     GTPWIDEFRELIIEFGNETWHNGVFEDWLGFRTRNAVWQGGQEYGMFARYLIENMQRS
                     PYWQQQKLAGRIRFALGDGYVRRDINGRMLTYGEEAMLECPQADVLAHANYVGPKWET
                     GDASSSTFDDHGVQATLLAFQTGGGAEFGEWQKVRDEMAAAGHAYDLAAYESGPSGYT
                     LPGQGNAEQVEVNERYGKSLAMGVAALDAWLGAAEG"
BASE COUNT          277 a          551 c          545 g          259 t
ORIGIN      
        1 cgtctacgtg gtcgatggcg ggccccggat cgagccgttc gactacgtcg tcgtggagcg
       61 ccgcttcggc agctacgaca tgaggtacac ccacccgcgc gtgcgcggca tcgggaatcc
      121 catggactcg cccaactggc actggcgcgg cgagcccggc caggtgcgct tctccctcgt
      181 gccacaccca cagccacttc ccgaggccat ggtcgatcct ggcgagacgt gcctccaggt
      241 cgatgcgctc gtggccgagg aagcctccat caaccagatc gtgttcatcg gcaccgacca
      301 tggcggcgag tcgatctggt acggccagct cgagccgggg aggcattatc gcttgagtgt
      361 ctggctgcgc caggaggggc tggccaatgg cggagccgtc cggttctcct acggccaggg
      421 ataccccggc atctcacacc acttcggcgt caccaaccag tgggcggagt acacctgcga
      481 gttcgatggc cccgagcggc cggcggaccc gtggcacttc ggtcacacat tcaccttcac
      541 cggccccggc acgctctgga tggacaactg ccgcatcgcg cgcgtcgacc ggccagagga
      601 tgccaccaag ccgtacgtgc cgaacgcaac cgtgctcgac gagctgaagg ccgcgtcgcc
      661 ccccgccggt ccgaagggca tgcaacggtg ctgggtactg gcgcgcgatt cgaccatgga
      721 ccacctgctc agctggtatg gcaactcgca ggtgcggccc gactggtcca cgagtatcag
      781 cgggaccgtc gacatgacca tgccgcagct cctcgagttc tgtcggctca ctggccccga
      841 tccgacgcag cgggtggtgc cgtggatcgt gattcagcac attctgcatg atgaggatga
      901 ctggctggcg ctggtggagt acctcgccgc gccctatgac cccgcggcgg actcaccaga
      961 gaccaagcct tgggccttcc gccgctttcg gcagcgcggc accggcacgc cgtggatcga
     1021 cgagttccgc gagctcatca tcgagttcgg gaacgagacc tggcacaacg gtgtgttcga
     1081 ggactggctc ggtttccgca cccgcaacgc cgtctggcag ggcgggcagg agtacggcat
     1141 gttcgcccgc tacctcatcg agaacatgca gcgcagtccc tactggcagc agcagaagct
     1201 cgccggccgc atccgcttcg cgctcggcga cgggtatgtc cggcgggaca tcaacggccg
     1261 gatgctgacc tacggtgagg aggccatgct cgagtgccca caggccgacg ttctcgccca
     1321 cgcgaactat gtcgggccaa agtgggagac cggcgatgcc tccagcagca ctttcgacga
     1381 tcatggcgtg caggccactc tgctggcgtt ccagactgga ggcggcgccg agttcgggga
     1441 gtggcagaag gtccgcgatg agatggccgc ggccgggcac gcctacgact tggccgccta
     1501 cgagagcggc cccagcggct acaccctccc cggccaaggc aacgccgagc aggttgaggt
     1561 gaatgagcgg tacggcaaga gtctggccat gggcgtggct gcgctcgacg cctggctcgg
     1621 cgcggcggaa gg
//
LOCUS       DAOSPL010001247         1631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_75299_length_1631_cov_2.534264, whole genome shotgun sequence.
ACCESSION   DAOSPL010001247 DAOSPL010000000
VERSION     DAOSPL010001247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75299_length_1631_cov_2.534264"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1631
                     /locus_tag="PLQ54_18680"
     CDS             <1..>1631
                     /locus_tag="PLQ54_18680"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOS95344.1"
                     /translation="EAARRVKVRYRPLPAVLSVEEAARDGSPLVHPGVDGFPGGAFGM
                     ELVLDGNRCYEEETVDGDVDAAFEHADVVLDETYRTAPQHQASIEPHAAVAAPRGDGG
                     VAIWTSTQAPFIVRQGVAAYLGLPMSQVRVVAMETGGAFGGKVSVFIEPMVAALALKT
                     GQPVRMVYSRQEELEDARPRSATVIRVRTAAQADGTLLAREVDFLADSGAYADWTPAV
                     VNSSLRACRGPYRVPNLRAKGAAVYTHKFNTGPFRAPGYPQLTFALESHMDELAAKLG
                     MDPMELRRKNLLRNGEKTFSGTELKTDVLMRCFDTVTKAIADDPADEPHTAWGLACGE
                     WEIGGFPASALLKVNEDGTVSLSVGSTDLTGSLTSIAQVVADELGIEMTELSASCIDT
                     DTSPLAPLSGGSMVTYNMTHVAREGAKQLADELKKRAATALEAKPSQMEVSGLKVRLK
                     KDPSKSVSFAGLVANEPAGITITTPALPATHSFAVHAVKVSVSPETGEVRVLRAISAL
                     DCGKAINPNMVKGQMCGGLVQGLGECLWEEIRFDG"
BASE COUNT          283 a          540 c          547 g          261 t
ORIGIN      
        1 ggaggccgct cgccgggtga aggtgcgata ccgcccgctt cccgccgtgc tgtcggtcga
       61 ggaagcagcg cgcgatggat cgccactggt tcaccccggt gtcgatgggt tccccggcgg
      121 cgcattcggc atggagctcg tgctcgacgg caaccggtgc tatgaagagg agaccgtcga
      181 tggcgatgtg gatgccgcct tcgagcacgc cgacgtggtg ctggacgaga cataccgcac
      241 cgcccctcag caccaggcat ccatcgagcc gcacgcggcc gtggcggcac cccgggggga
      301 cggtggagtc gccatatgga cgtcgaccca ggctcctttc atcgtccgcc agggggtcgc
      361 agcctacctg ggactgccca tgtcgcaggt ccgcgtggtc gccatggaga cgggaggggc
      421 tttcggtggc aaagtctccg tcttcatcga gccgatggtc gccgcgctgg cgctcaagac
      481 cggacagccc gtgcggatgg tctactcgcg ccaagaggag ctcgaagacg cacgcccccg
      541 gtcggctacg gtgatccgcg ttcgcacggc tgcccaggcc gatggtacgc tgctcgctcg
      601 tgaggtggac ttcctcgccg acagcggcgc gtacgccgac tggactccag ccgtggtaaa
      661 cagctcactc cgggcttgcc gtggcccata ccgcgtcccg aatctgcggg ccaagggcgc
      721 cgccgtctac acccacaagt tcaacacggg ccccttccgt gcgccgggct acccccagct
      781 cacctttgct ctcgagtcgc acatggatga gttggccgcc aagctgggca tggatcccat
      841 ggagttgcgg cgcaagaacc tgctccggaa cggagagaag accttctcgg gtaccgagct
      901 caagaccgat gtcctcatgc gctgcttcga taccgtgacg aaggccatcg ccgatgatcc
      961 ggccgatgaa ccgcataccg catggggtct ggcgtgcggc gagtgggaga tcggcgggtt
     1021 ccccgccagt gccctgctca aggtgaacga ggacggcacg gtgtcgctct cggtaggctc
     1081 caccgacctc acggggtcac tcacatccat cgcccaggtg gtggccgacg aactggggat
     1141 cgagatgacc gagctctcgg cctcgtgcat cgacaccgac acatcgcctc tcgcgccgct
     1201 gagcggcggc agcatggtga cctacaacat gacccatgtg gcgcgggagg gcgccaagca
     1261 gctggcggac gagctcaaga agcgggccgc caccgccctc gaggccaaac cgtcgcagat
     1321 ggaggtctcc ggcctgaaag ttcgcctgaa gaaggatccg agcaagtcgg tttcgttcgc
     1381 cggcctggta gcgaacgagc cagccggcat caccatcacc accccggcgc tgcctgcaac
     1441 gcactcgttt gccgtccatg cggtcaaggt gagcgtctca ccggaaacgg gcgaagtgcg
     1501 cgtgctgcgt gccatcagcg cgctcgactg cggcaaggcc atcaacccga acatggtcaa
     1561 agggcagatg tgcggcgggc tggtccaggg cctgggcgaa tgcctctggg aggagatccg
     1621 gttcgacggt g
//
LOCUS       DAOSPL010001248         1629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_75404_length_1629_cov_2.855146, whole genome shotgun sequence.
ACCESSION   DAOSPL010001248 DAOSPL010000000
VERSION     DAOSPL010001248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75404_length_1629_cov_2.855146"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="PLQ54_18685"
     CDS             complement(<1..1049)
                     /locus_tag="PLQ54_18685"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOS95345.1"
                     /translation="MPGQNHRPPRRPNIVYILADDLGWADLGCTGSRFHQSPHLDRLA
                     TQGVRFETCYAAAPVCSATRASIQTGKYPARLHLTDFIPGHGRPWARLRVPDFRQQLP
                     LEEISLARALKPAGYACANIGKWHLGGPGFGPAEHGFDHVFSTGVNDDDKQVSALTED
                     ALGFIRAHRYEPFLLMLCHYTVHIPLEAKQALIAKYAALAEPGAPQSNPTYAAMIEVL
                     DDSVGRVLAELDTLGLTDDTIVLFGSDNGGLVHWYIGDGPEVTSNAPCRGEKGTIHEG
                     GLRVPLIVRWPGHTQVGRVCDVPVSTIDLFPTLMEATGVPYNHSVDGLSLMPLLTGAG
                     GIGRDTHYFHYPHYH"
     gene            complement(1106..1342)
                     /locus_tag="PLQ54_18690"
     CDS             complement(1106..1342)
                     /locus_tag="PLQ54_18690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95346.1"
                     /translation="MAIADTGNVGSNGNLDESGFFSNCGNEAYSENWPSTCPWRLIWR
                     ARNRHNGGLNAGFLDGHAKWIKLSQYTSTTYYLP"
     gene            complement(1379..>1629)
                     /locus_tag="PLQ54_18695"
     CDS             complement(1379..>1629)
                     /locus_tag="PLQ54_18695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95347.1"
                     /translation="NVKQMSLALEMYTQDADECLPPMPCGCNYVPGNPHCWYPRIQPY
                     IKNTQVTVCPSIGTIGRNVPCGAEIPSLAYGLNLQFRN"
BASE COUNT          262 a          486 c          584 g          297 t
ORIGIN      
        1 ttgtggtaat gcgggtaatg gaagtagtga gtatcgcgtc cgatgccgcc cgcaccggtg
       61 agtaggggca tcaggctgag tccgtcgact gagtggttgt acggaacgcc ggtcgcctcc
      121 atcagcgtcg ggaacaggtc gatggtcgac acgggcacgt cacacacacg gcccacctgc
      181 gtgtgcccgg gccagcgcac gatgagaggc acgcggaggc ccccctcatg gatggtaccc
      241 ttctcgcccc ggcagggcgc gttcgaggtc acctcgggcc catctccgat gtaccaatgg
      301 accagtccgc cgttgtcgga accgaacagc acgatggtgt catcggtcag ccccagggtg
      361 tcgagttcag ccaggacgcg gcccaccgag tcgtcgagca cctcgatcat tgccgcatag
      421 gtcgggttgc tctgaggtgc tcccggttcc gccaacgccg cgtacttggc gatgagcgcc
      481 tgcttggctt cgagcgggat gtgcaccgtg tagtgacaga gcatcaggag gaacggctcg
      541 taccggtggg cgcggatgaa cccgagtgcg tcctcggtga gtgcactcac ctgcttgtcg
      601 tcatcgttga caccggtgct gaacacgtgg tcgaaaccgt gctcggccgg cccgaatccc
      661 gggccaccca ggtgccactt gccgatgttg gcgcaggcgt agcctgccgg cttcagcgcc
      721 cgcgccaggc tgatctcctc gagcgggagc tgctggcgga aatcgggtac gcgtagccgg
      781 gcccagggcc gaccgtggcc cgggatgaag tccgtcaggt gaagccgtgc cgggtacttg
      841 cccgtctgga tgctggcccg cgtcgccgaa cagacgggcg cggccgcata gcaggtttcg
      901 aagcgcacgc cctgcgtcgc gagccggtcc agatggggtg actggtgaaa gcggctgcct
      961 gtgcagccca aatcggccca gccgaggtca tcggcgagga tgtagacgat gttgggccgg
     1021 cgcggcggac ggtggttctg acccggcatg gaacggctcc tccagcgcga gcgcgccccg
     1081 gaggagccgt cggcatccgg cggggctacg gcagataata ggtggtgctg gtgtactgcg
     1141 acagcttaat ccatttcgcg tggccgtcca ggaaccctgc attgaggccc ccgttgtggc
     1201 ggttgcgggc acgccagatc aggcgccagg gacaggtgga cggccagttc tccgagtagg
     1261 cctcgttgcc gcagttggag aagaagcccg actcgtccag gttgccattg ctgccgacat
     1321 tgcccgtgtc ggcgatggcc atggtcgtgg ccggcgcggg ccattgggag atattcggct
     1381 agtttctgaa ctggaggttc agcccgtagg cgaggctcgg gatctcggcg ccgcagggca
     1441 cgtttcggcc gatggtgccg atgctggggc agacggtgac ctgagtgttc ttgatgtagg
     1501 gctggatccg gggataccag cagtgcgggt tgccggggac gtagttgcag ccgcagggca
     1561 tgggtggcag gcattcgtcg gcatcctgcg tgtacatctc cagtgccagt gacatctgct
     1621 tgacgttgg
//
LOCUS       DAOSPL010001249         1629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_75425_length_1629_cov_2.215375, whole genome shotgun sequence.
ACCESSION   DAOSPL010001249 DAOSPL010000000
VERSION     DAOSPL010001249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75425_length_1629_cov_2.215375"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="PLQ54_18700"
     CDS             complement(<1..1100)
                     /locus_tag="PLQ54_18700"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOS95348.1"
                     /translation="MRFHWLWGLLLVTLGASVWPPERLAAAAGRPEVPAAPATLAACI
                     DRALERSPRLALAHAAARAADTGVDLARSAGGLHAALSGEVRGTTPTPLVQIPGQEPR
                     EVIPALDASLGLAIEVPLDVGRRLRSERRAAQAGADAAWARYEQARDQLVFEVVRAYY
                     GVLQADAFRDATAAAVDAAEESRRVTRARVAAGMANDLESQRADTALAKAREQLALAE
                     SRAAEARAALATVIGMPGAPVGELQIVFLEPDRIPDPEESIARAQAERPELAAALADL
                     RQAASRLRAADAADRPAWALSGGVKAQRASLIRVPFAATIGLGFTWPFGDHGRADALV
                     DQAEASRDGARSAVADTAAAIELQVQQSVLAI"
     gene            complement(1207..>1629)
                     /locus_tag="PLQ54_18705"
     CDS             complement(1207..>1629)
                     /locus_tag="PLQ54_18705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOS95349.1"
                     /translation="RRVVDALCDVHAELIEPDYGAAFAYLSRRSRRRSLTVLFTDLVD
                     SAASEALLAGVASLRPHHLPMLVSIRDQSLERATTDPVVDAAGVYRRAVAEEVLEDRE
                     RALARLRARGVVVVDSAPEQLSVMTVNKYLLLKAENRL"
BASE COUNT          248 a          600 c          554 g          227 t
ORIGIN      
        1 tcgatggcca gcacggactg ctgtacctgc agctcgatgg ccgctgccgt gtcggccacc
       61 gctgaacggg caccgtcccg ggatgcttcc gcctggtcaa ccagcgcgtc cgcccggccg
      121 tgatcaccaa aaggccacgt gaacccgagt ccgatcgtcg ccgcgaaggg cacgcggatc
      181 aggctggcgc gctgggcctt gacaccgccc gagagcgccc aggccgggcg atctgccgca
      241 tcagcggccc gtaggcgaga cgccgcctgc ctcagatcgg ccagggccgc cgccagctca
      301 gggcgctctg cctgtgcccg cgcgatgctc tcttccggat cgggaatccg gtcgggttcg
      361 aggaacacga tctgtagttc cccaactggt gcgccgggca tgccgatcac ggtggccaag
      421 gccgcacggg cctcggcggc gcggctctcg gccagcgcca gctgttcgcg ggcctttgcc
      481 aacgcggtat cggcccgctg cgactccagg tcattggcca tccccgccgc gacgcgggcc
      541 cgagtcaccc ggcgggattc ctccgcagcg tcaacggccg ccgctgtggc gtcgcggaag
      601 gcgtcggcct ggagcacgcc gtagtaggcc cgaacgacct cgaacaccag ctggtcccgg
      661 gcctgctcat atcgcgccca ggccgcgtcg gcgcctgcct gcgccgcccg ccgctcgctc
      721 cgcagccgac ggcccacgtc caagggcacc tcgatggcca ggcccaggct ggcatccagc
      781 gccgggatga cctcgcgcgg ctcctggccc gggatctgca ccaacggagt aggcgtggtg
      841 ccccgcactt cgccgctcag ggctgcgtgc agtccgccgg cgcttctggc gaggtcgaca
      901 cccgtgtccg ccgcccgggc ggcggcgtgg gccagggcca gcctggggct acgctcgagc
      961 gcacggtcga tgcaagccgc caaggttgcg ggcgcggcgg ggacttcagg gcggccggcc
     1021 gcggcagcca ggcgctctgg tggccagacc gaagctccga gagtgaccag cagcaggccc
     1081 caaagccagt ggaaacgcat gtcaacagcc ccagcaggat gaagtggtcc tcagaagcga
     1141 tccgtggcga ccccgcgggc gccacctatt cgcaccgcac tgccggactc ctccgccgtg
     1201 ccccacctag agcctgttct cggccttcaa gagaaggtac ttattcactg tcataacaga
     1261 gagttgctcc ggtgcggagt cgacgaccac cacgccgcgc gctcgaagcc gggccagcgc
     1321 gcgctcacgg tcctcgagca cctcttcggc gaccgcccgc cggtagacgc cggccgcatc
     1381 cactaccgga tccgtggtcg cccgctccag gctctggtcg cgaatggaga ccagcatggg
     1441 gagatggtgg ggacggagcg aggccacgcc agcgagcagt gcctcggacg ccgccgagtc
     1501 caccaggtcg gtgaacagca cggtcaggct tcgtcgccgg ctgcgtcggc tcagataggc
     1561 gaatgccgcg ccgtagtcgg gttcgatgag ctcggcatgc acgtcacaca atgcgtcgac
     1621 gacccttcg
//
LOCUS       DAOSPL010001250         1628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_75477_length_1628_cov_3.122695, whole genome shotgun sequence.
ACCESSION   DAOSPL010001250 DAOSPL010000000
VERSION     DAOSPL010001250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75477_length_1628_cov_3.122695"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLQ54_18710"
     CDS             <1..320
                     /locus_tag="PLQ54_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOS95350.1"
                     /translation="YSGAVIGHFQHRAVAVGTTCARVLETCAQPDGLVVPQSGSTRLF
                     ITPGYEFRCVDCLLTNFHLPRSTLLMLVFAMTGVELGQRAYAEAVARRYRFYSYGDAM
                     LIM"
     gene            complement(408..1112)
                     /locus_tag="PLQ54_18715"
     CDS             complement(408..1112)
                     /locus_tag="PLQ54_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOS95351.1"
                     /translation="MRSGTARGFTLIELLVALAIIALLVGVLLPAYAGSRRKGWEATC
                     TSNLRQLALAWRAYCDDWDGPPATLATIREPYVRTAAIFRCPADPDPNGMAKSGSVDA
                     LVNDGIDPNTIFPIGVTYAYDSMWGAGAHWQSTITKALEMVPTAGLIACPCHGEHLPV
                     PRGYSGPTSFTGLVLRARPDGSVIRARQDNRDPWGQNPDRVLYGYPCGAGEIVFPPTK
                     ECIEYRRQHCPESLEL"
     gene            complement(1185..>1628)
                     /locus_tag="PLQ54_18720"
     CDS             complement(1185..>1628)
                     /locus_tag="PLQ54_18720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95352.1"
                     /translation="GMAAGELVARATPMVTHHGYSDLNRYVGALCGAACCLGLTLLGP
                     RPVRDVLLVPVVAKVSAALVGCYSYARLGDLRTFFSSVLPGATVGQGLHWLIVEDIPL
                     GLLTLAGTYPVALWIRRRAAEAEAEAGAARAARAARNEDGTWNVG"
BASE COUNT          262 a          564 c          548 g          254 t
ORIGIN      
        1 ggtattccgg ggccgttatc ggccacttcc aacaccgcgc ggtcgccgtc ggcaccacgt
       61 gcgcgcgagt gctggagacc tgtgcacagc ccgacggcct ggtggttccg cagtcgggct
      121 cgacacggct gttcatcacg ccgggatacg agttccggtg cgtcgactgt ctgctgacga
      181 acttccactt gccccggtcg actttgctga tgctggtctt cgcgatgacg ggcgtcgagc
      241 tggggcagag ggcctatgcg gaggccgtcg cgaggcgcta ccgcttctac agctacggcg
      301 acgcgatgct gatcatgtag gcgggcgcct gcacgacggc tatgccgcgg cgacggaggc
      361 cgcggctcgc cctgcggcag tgacctcagg actcgcatcc caggggccta gagctccaag
      421 ctctccgggc agtgctgcct gcggtactcg atgcactcct tcgttggtgg gaatacgatc
      481 tcgcctgcgc cgcacgggta cccgtacaga acccggtcgg ggttctgccc ccaggggtcg
      541 cggttgtcct gcctcgcgcg gatcaccgaa ccgtccggac gagcgcgaag gaccagcccc
      601 gtgaagctcg tgggcccgct gtagccacgc ggcacgggca gatgctcgcc gtggcagggg
      661 catgcaatga gcccggcggt gggcaccatc tcaagcgcct tggtgatggt gctctgccag
      721 tgcgcgccgg cgccccacat cgagtcatac gcgtaggtga ccccgatcgg gaagatggtg
      781 ttcggatcga tgccgtcgtt cacgagcgcg tccaccgacc cgctcttggc catcccgttg
      841 gggtcgggat cggccgggca acggaagatc gccgccgtcc gaacgtaggg ctcccggatg
      901 gtggcgagcg ttgccggagg tccgtcccag tcgtcgcagt acgcgcgcca ggcaagggca
      961 agttggcgca ggttcgacgt gcacgtcgcc tcccagccct tccggcgcga ccccgcgtag
     1021 gccggcagca ggacacccac caacagcgcg atgatcgcca gcgccaccag cagctctatc
     1081 aatgtgaacc cacgagcggt tccgctacgc atgtgcggtc acccctctca ggacgccgta
     1141 gccgcggagg gcgcgggggt agcccacgcc ctccgcggct acggctaccc cacgttccac
     1201 gtgccgtctt cgttccttgc cgctctggcc gctctcgcgg ccccagcctc agcctcggct
     1261 tcggccgcgc gcctgcggat ccacagggcc acggggtagg tacctgctag agtcagcagg
     1321 ccgaggggga tgtcctcgac gatcaaccag tgaagcccct ggccgaccgt cgcgccgggc
     1381 aagacagacg agaagaacgt gcgcaggtca cccaggcggg cataggagta gcagccgacc
     1441 agcgccgcag agactttggc cactactggt acgaggagca cgtcgcggac aggccgaggg
     1501 cccaagagcg tgagccccag gcagcaagcc gccccacaca gggccccgac gtaccggttc
     1561 aggtccgagt agccatgatg agtcaccatc ggtgtggctc gggccacaag ctcgcccgct
     1621 gccatacc
//
LOCUS       DAOSPL010001251         1628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_75488_length_1628_cov_2.782581, whole genome shotgun sequence.
ACCESSION   DAOSPL010001251 DAOSPL010000000
VERSION     DAOSPL010001251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75488_length_1628_cov_2.782581"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PLQ54_18725"
     CDS             <1..553
                     /locus_tag="PLQ54_18725"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HOS95353.1"
                     /translation="DAVLVGVGTVLADDPALDVRLCDGPFRQPLRVVVDTHARIPQEA
                     TLLRQPDGLVVAVGPSAPAERVDGLRSCGVRVDVLPESEGGIDLAALLRHLGDAGTLC
                     VLVEGGARIHASMLRGGLIDRVVLFVAPVIAGAGGVPLSESLGARRVWDGVRLTDLRA
                     EPCGPDLRIEAYVEGHDPSCSPD"
     gene            571..1191
                     /locus_tag="PLQ54_18730"
     CDS             571..1191
                     /locus_tag="PLQ54_18730"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOS95354.1"
                     /translation="MRRVQRSGQSARATIAARRILSDLKSGDSVAVSGACVTAVRVLG
                     DSFTCDLSAETLSRTTLCLKRLPAPVNLERASLPTTRMGGHLVLGHVDGIGTVVSLVR
                     SGDGLEMVFEMPDELRPYVVDKGSIAVDGVSLTPFAVAGRTFRVALIPTTLGETTLQH
                     LSPGDPVNLEADVMAKYAVGARDDVPPQGPPSGVTAELLRRTGFLD"
     gene            1403..>1628
                     /locus_tag="PLQ54_18735"
     CDS             1403..>1628
                     /locus_tag="PLQ54_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95355.1"
                     /translation="MNQIPPGLFLPGQWFVGGRAAAAARRVLESADGSRLPAFMPADE
                     ARRRPELPSAGRIGAVAVAGLADSVNPCALT"
BASE COUNT          211 a          570 c          559 g          288 t
ORIGIN      
        1 cgatgccgtg ctcgttggtg tcggaaccgt tctcgccgat gacccggctt tggacgtccg
       61 cctgtgcgac ggccccttcc ggcagcctct gcgggtggtc gtcgacacac acgcccggat
      121 cccgcaagag gccaccctgc tccgccagcc tgacggcctt gtggtggccg tgggcccttc
      181 ggctcccgct gaacgcgtcg acgggctgcg ctcttgcggc gtccgtgtcg atgtgctccc
      241 tgagagcgag ggcggcatcg acctggccgc gctcttgcgg cacctcggag acgccggtac
      301 actctgcgtg ctcgtcgagg gtggcgcgcg catccatgcg tcgatgctgc gaggcgggct
      361 gatcgaccgc gtcgtcttgt tcgtggcacc cgtaatcgcc ggggccgggg gcgttccgct
      421 gagcgagtca cttggcgcgc ggcgagtgtg ggatggcgtc cggctcactg atctgcgcgc
      481 cgagccctgc gggcccgatc tgaggatcga ggcctacgtg gaggggcacg acccaagctg
      541 ttcaccggac tgatcgagga gatcggcgtc ttgcgtcgcg tccagcgctc ggggcagagc
      601 gctcgggcca ccatcgccgc gcgccggatc ctgtccgacc tgaagtccgg cgacagcgtt
      661 gccgtgtcgg gcgcgtgcgt caccgcggta cgggtgctcg gggactcgtt cacctgcgac
      721 ctctcggccg agaccctgtc gcgcacgacc ctgtgcctca agcgtctgcc tgccccggtg
      781 aacctggagc gcgcatcgct gccaacgacc cgcatgggcg gtcatctggt gttgggccat
      841 gtagacggca tcgggacggt ggtctcgctg gttcgctccg gcgatggtct cgagatggtg
      901 ttcgagatgc ccgacgagct gcggccctat gtggtcgaca agggctcgat cgcggtcgat
      961 ggcgtgagcc tgacgccctt cgccgttgcc ggtcgcacct tccgtgtcgc cctgattccc
     1021 accacgctag gagagacgac gctgcagcac cttagcccgg gcgatccagt caacctcgag
     1081 gccgacgtga tggccaaata cgccgtgggg gcgcgcgacg acgttccccc ccagggaccg
     1141 ccgtccggtg tgaccgccga actgctccgc cgcaccggct tcctggacta ggccccgtcc
     1201 tgcgctggcg aggtaccgtg gaggcccccc attgccgccg gctgcagggg atcgcgaggg
     1261 cattggcggc gcaaaccgct gcactctgct taggttgagt cagtccccag aaaaacccct
     1321 tgccccccgc ctgatgactt acctttgttg atgtgcgttg acaaccagcc aacgtacgga
     1381 tggctcccag cgccggccgg ccgtgaacca gatccctccc gggctcttcc tgccgggcca
     1441 gtggttcgtc ggcggacgtg cggccgcggc ggcgcgacgc gttctcgagt ccgcggacgg
     1501 ctcgcgcttg cccgcattca tgcccgcaga cgaggctagg cgccgccccg aacttccatc
     1561 tgcgggcagg atcggcgcag tggcggtggc cggcctggcc gatagcgtca atccctgcgc
     1621 gctcaccg
//
LOCUS       DAOSPL010001252         1627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_75544_length_1627_cov_2.840331, whole genome shotgun sequence.
ACCESSION   DAOSPL010001252 DAOSPL010000000
VERSION     DAOSPL010001252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75544_length_1627_cov_2.840331"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PLQ54_18740"
     CDS             <1..280
                     /locus_tag="PLQ54_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOS95356.1"
                     /translation="LGTGPRIVGRVMATTDEDLPWWRIVRADGGMADPLVDRAIPHWR
                     EEGITFTGNRVRMTRHRADLAALADAAEAALGRLPGAGEPGHAPAPSC"
     gene            complement(367..1230)
                     /locus_tag="PLQ54_18745"
     CDS             complement(367..1230)
                     /locus_tag="PLQ54_18745"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HOS95357.1"
                     /translation="MLLFYIDECGDHSLRVAPQSDPPQLKTGVSPWFVLGAVGIRDTS
                     RQPLAAAIKAIKERHFGESVLLAPWAASEIKGRYLSRAARSVANGRTLESPAGYPVLD
                     TVEKVDALIEDLGLLFTTFRPLVFAVGVNKKALLRRTDPVSPLGAAYAYLEQRVALTL
                     QRVYQGEGAVLVADQQTQHEAYFRSGEMNETRDALTAKLPTRPQFDLVLDKPLWVDTD
                     LSTWDREILQLADIVAYSVTECLVTGRPPQARSHLWAQIRHQLAIQWSTGAIEGGGLA
                     IFPRPRKYPET"
     gene            complement(1311..>1627)
                     /locus_tag="PLQ54_18750"
     CDS             complement(1311..>1627)
                     /locus_tag="PLQ54_18750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95358.1"
                     /translation="NWSVAMEALLTLCRDDTIIVPGHGALTEPHYIAWQRAGLAAVYG
                     QCEWLVTQGVPEPDAYAHPELQWPWDEAWVRAAIHQAYAEIAPNGVVTRRILPLSATP
                     KD"
BASE COUNT          271 a          525 c          539 g          292 t
ORIGIN      
        1 cctcggtaca ggcccgcgga tcgtcggaag ggtcatggcg accaccgacg aagacctgcc
       61 ctggtggcgg atcgtccggg ccgacggtgg catggccgac cccttggtgg accgcgcgat
      121 cccgcactgg cgtgaggagg gcatcacctt cacaggcaac cgggtacgaa tgacccgcca
      181 ccgcgcggac ctcgcagccc tggcagacgc cgcagaggcc gccctcggac gactgccggg
      241 cgccggcgaa cccggccatg cacctgcacc ttcctgttga gcccactcag tgcgtgtgtc
      301 agaatggctt caccggcgtg agccgagacc ttctggaatc cggtgggtct tggcattgcg
      361 ccggttctac gtttccgggt acttgcgcgg tctgggaaag atggccaggc ctcccccttc
      421 tatcgccccg gttgaccact ggatcgcgag ctggtgtctg atttgggccc agaggtgact
      481 ccgtgcctgc ggaggtctgc ctgtgaccag gcactcagtg actgagtagg ccacgatgtc
      541 agccagttgg aggatctccc ggtcccaggt cgacaggtcg gtatcgaccc atagaggctt
      601 gtcgagcaca agatcgaact gcggtcgcgt gggcagcttt gctgtcaggg catcccgggt
      661 ctcgttcatc tccccggatc ggaagtacgc ctcatgctgt gtctgctggt cggcgacaag
      721 gacggctcct tctccctgat agaccctctg cagtgtgagc gcgacgcgct gctcgaggta
      781 ggcgtaggcg gcgcccaggg gtgagacggg atctgtgcga cgcaggaggg ccttcttgtt
      841 cacaccgacg gcaaagacca gaggtcggaa cgtcgtgaac aacagtccca agtcctcgat
      901 gagggcatca accttctcca ccgtgtccag gaccgggtag ccggcagggc tctccaacgt
      961 gcgcccgttc gccacgctcc gggccgcccg gctgaggtag cgtcccttga tctccgaagc
     1021 tgcccagggg gccaagagaa cagactcacc gaagtgacgc tctttgatgg ccttgatcgc
     1081 cgctgccaga ggttgccgac tcgtatctct gatgccgacg gctcccaaca cgaaccaggg
     1141 gctgaccccg gtcttcaact gcgggggatc cgattggggc gccaccctga gcgaatggtc
     1201 gccgcattca tcgatgtaga acagaagcac gagtactcca actggacggg gtgggctatg
     1261 tcgtgaccga ccgtaacagc ggtctcagac agtgtcctgc gtcgatgctt ctagtccttc
     1321 ggagttgccg agaggggcag gatcctgcgg gtgaccacgc cgttgggggc gatctctgcg
     1381 tacgcctggt ggatcgcggc acggacccac gcctcgtccc agggccactg gagctccggg
     1441 tgggcgtagg cgtcgggctc ggggacgccc tgcgtgacga gccactcgca ctgtccgtac
     1501 acagcggcga gtccggcccg ctgccacgcg atgtagtgcg gctcggtcag cgcaccgtgg
     1561 cccggcacga tgatcgtgtc gtcccggcac agcgtgagca gggcctccat cgcgaccgac
     1621 cagttct
//
LOCUS       DAOSPL010001253         1626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_75620_length_1626_cov_2.806493, whole genome shotgun sequence.
ACCESSION   DAOSPL010001253 DAOSPL010000000
VERSION     DAOSPL010001253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75620_length_1626_cov_2.806493"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1626
                     /locus_tag="PLQ54_18755"
     CDS             <1..>1626
                     /locus_tag="PLQ54_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95359.1"
                     /translation="EGNTRATAENRFAVRLDRIALDRRDALEFPVDYAQALADARAAL
                     AVVLGRIGPGAATLRSPGFHPWTQPVVPERHPLFATAEPKPVTRETLGYGLHFTGARG
                     IDAATLDQFHTTYDFGDICWPHIGILPLREKHASDADYEAALGELESRLEDVKRRGLF
                     LFDIWGYVPDMPEYPWRVAPEHIALLQRVLGDRFLGCDNGEQDGRYIGSYADRDPHTN
                     RREGWDSFVRWDQRIAGDNGNYINATGSLNFSHYYGERGARTLGLETAQGLPSDTLMF
                     AFLRGAAKQYGRLTTQATSVWNRYGYNMYSDRRTEGPNGYGLGPSKGCSLSLHKRLML
                     SSYSGGDSIVGTETAQFTADTTDAGSPELSPLGAQHLAIRQWADAHPDRGVMYTPVAL
                     MLDFHSGWTVPRHLYRGDKYKVWGKLPYEKGDYLADNVFRMVWPGYEDCSYLRNERGF
                     LTPTPYADLFDVITNRCHADILKQYTAVVLLGDVDMPTEVVERLSSFVRAGGDLVVDA
                     PRARGLGEELAGLTVGDPERGVLSHVLASGQTY"
BASE COUNT          274 a          573 c          526 g          253 t
ORIGIN      
        1 acgagggcaa tacgcgagcg actgccgaga accgcttcgc cgtgcggctc gaccgcatcg
       61 ccctcgaccg gcgcgacgcg ctcgagttcc cggtggacta tgcgcaggcc ctggccgacg
      121 cccgggcagc actcgccgtc gtgctgggtc ggatcggccc gggcgcggcg actctccgct
      181 cgccgggctt ccacccgtgg actcagccgg tcgtgcccga gcggcaccct ctgttcgcca
      241 ccgccgagcc gaagcccgtc acgcgcgaga cgctgggcta cggactccac ttcaccggtg
      301 cccgagggat tgacgccgcg acgctcgacc agttccacac cacatacgac tttggcgaca
      361 tctgctggcc ccacatcggc atcctgccgc tccgcgagaa gcacgcctcc gatgccgact
      421 atgaggctgc cctgggcgaa ctcgagtcgc gcctggagga tgtgaagcgg cggggcctct
      481 tcctgttcga tatctggggc tacgtgcccg acatgcccga gtacccctgg cgcgtggctc
      541 ccgagcacat cgctctgctg cagcgagtcc tgggcgatcg gttcctcggg tgcgacaatg
      601 gcgagcagga cggccggtac atcgggtcct acgcggaccg cgatccgcac acgaaccggc
      661 gcgagggatg ggacagcttc gtgcgctggg accagcgcat cgccggcgac aacggcaact
      721 acatcaatgc cacgggctcg ctcaacttct ctcactacta cggagagcgc ggggcgagga
      781 cgctggggct cgagacggca cagggcctcc ccagcgacac actgatgttt gcattcctcc
      841 ggggggccgc caagcagtac ggccggctga cgacccaggc cacctcagtg tggaaccgtt
      901 acggctacaa catgtacagc gaccggcgta ccgagggccc caacggctac ggtctaggcc
      961 ccagcaaggg ctgctcgctg agcctccaca agcgcctgat gctctcgagc tactctggcg
     1021 gcgatagcat cgtgggcacc gagacagcgc agttcactgc cgacaccaca gacgcgggct
     1081 caccggaact cagtccgctc ggcgcccaac acctggcgat caggcagtgg gccgacgccc
     1141 atcccgaccg tggggtcatg tacacgcccg tggctctcat gctcgacttc cacagcggct
     1201 ggacggtccc gcgtcacctg taccgcggcg acaagtacaa ggtgtggggc aagctgccct
     1261 atgagaaggg cgactacctg gccgacaacg tcttccgcat ggtctggccg ggctatgagg
     1321 actgcagcta cctgcgcaac gagcggggct tcctcacacc cacgccctat gccgacctct
     1381 tcgatgtcat cacgaaccga tgccacgcgg acatcctcaa acagtacacg gcggtcgtgt
     1441 tgctgggcga tgttgatatg ccgaccgagg tggtcgagcg cctcagctcc ttcgtgcggg
     1501 cagggggtga cctggtcgtc gatgcccctc gcgcccgcgg gctgggggag gaactggccg
     1561 ggctcacggt gggcgacccc gagcggggtg tgctcagcca tgtcctcgcc tcgggccaga
     1621 cctacg
//
LOCUS       DAOSPL010001254         1626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_75621_length_1626_cov_2.774029, whole genome shotgun sequence.
ACCESSION   DAOSPL010001254 DAOSPL010000000
VERSION     DAOSPL010001254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75621_length_1626_cov_2.774029"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1626
                     /locus_tag="PLQ54_18760"
     CDS             <1..>1626
                     /locus_tag="PLQ54_18760"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95360.1"
                     /translation="TFGDMMLLGRARRLEPGAFLSPDLSYDGRRVLFSYTENRQHRWI
                     WSPETAFHIFSVEVDGSNLRQLTDGEWDDFDPCWLPDGRVAFISERRGGYIRCFALLH
                     VRSHVLHSMEADGTDIAPLSFYETSEWHPSVNNDGMLVYTRWDYTDRENCLGSNFWIC
                     TPDGRDPRAPHGNYPYPWHTFEDNTHGDSRDGRPYTEMSIRAVPNSHRYIATAAPHHG
                     EAFGSLVMLDLRERDDGNMSQLRRITPYEPFPETESAARSQYAYGTAWPLSEDYYLCN
                     WWENLYLLDRFGNQVLLCENALVFGGETNYDMRLIDPIPLKPRPRPPIVAPGTEPMAD
                     HPATVALLNVYDSDLPLPPGTTIKWLRITQNILKPNPWMGVPMIGYQNENTPRIPLGI
                     VPVEADGSAYFEAPPGKELIFQALDENHMAVQSMRSVAFVHPGEHLTCIGCHEDPQRS
                     PTASGTPRALMRAPSRPQPEIGPIEPVSYYRTVKPVFERSCIPCHQREGKGPIDMSYS
                     ALEPYAFYFAGGMSRQTVLPTHGGSRTIPGRFGA"
BASE COUNT          296 a          557 c          509 g          264 t
ORIGIN      
        1 cacgttcggc gacatgatgc tgctcgggcg ggcgcgtagg ctggagccgg gcgcgttcct
       61 ctcgccggac ttgtcgtacg acggccggcg cgtcctcttc tcgtacaccg agaaccggca
      121 gcatcgatgg atctggtcgc ccgagaccgc gttccacatc ttcagtgtgg aagtcgatgg
      181 ttcgaacctc aggcagctca ccgacgggga gtgggatgac ttcgacccct gctggctgcc
      241 cgacgggcgc gtggccttca tctcggagcg acggggcggg tacatccggt gcttcgcgct
      301 gctccacgtt cgttcgcatg tgctgcactc gatggaggcc gacggaacgg acatcgcacc
      361 actgagcttc tacgagacga gcgagtggca cccgagcgtc aacaacgacg gcatgctcgt
      421 ctacacgcgg tgggactaca ccgaccggga gaactgcctg ggatcgaact tctggatctg
      481 cacgcccgac ggccgcgatc cgcgagcacc tcatggcaac tatccctatc cctggcacac
      541 gttcgaggac aacacgcacg gcgatagccg cgatgggcgc ccatacacgg agatgagtat
      601 ccgggccgtg cccaactcac accggtacat cgccacggcc gccccgcacc atggcgaggc
      661 gttcggcagc ctggtcatgc tcgatctgcg cgagcgggac gatggcaaca tgtcccagct
      721 gcggcgcatc acaccctacg aaccgttccc ggagaccgag agcgcggcgc gcagtcagta
      781 tgcgtacggc acggcctggc cgttgagcga ggactactac ctctgcaact ggtgggagaa
      841 cctgtacctg ctcgaccgct tcggcaacca ggtgctcctg tgcgagaacg cattggtctt
      901 cggcggtgag acgaactatg acatgcggct catcgacccc atcccgctca agcccaggcc
      961 ccggccaccg atcgtggcgc cgggcaccga gccgatggca gaccacccgg cgacggtggc
     1021 actcctcaat gtctacgact cggacctgcc tctcccgccg ggcacgacca tcaagtggct
     1081 gcggatcact cagaacatcc tgaaaccgaa cccctggatg ggtgtcccga tgattgggta
     1141 tcagaacgag aacacgccac gcataccgct ggggatcgtg ccggtggagg cggacggcag
     1201 cgcctacttc gaggccccgc cggggaagga actgatcttt caggctctcg acgagaacca
     1261 catggccgtg cagtcgatgc gctccgtggc attcgtccac ccgggcgaac acctcacgtg
     1321 catcgggtgc cacgaggacc cccagcggtc gcccacggct tccggcacgc cgcgagcctt
     1381 gatgcgcgcg ccctcgcgtc cgcagcccga gatcggcccc atcgagccgg tctcctacta
     1441 ccggaccgtg aagccggtgt tcgagcgatc ctgcattccg tgccaccaga gagagggcaa
     1501 aggaccgatc gacatgagct acagcgcctt ggagccctac gcgttctact tcgcgggtgg
     1561 catgagccgt cagacggtgc tgccgaccca tgggggctcg cgcaccatcc caggccgctt
     1621 cggtgc
//
LOCUS       DAOSPL010001255         1626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_75642_length_1626_cov_2.348822, whole genome shotgun sequence.
ACCESSION   DAOSPL010001255 DAOSPL010000000
VERSION     DAOSPL010001255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75642_length_1626_cov_2.348822"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PLQ54_18765"
     CDS             complement(<1..716)
                     /locus_tag="PLQ54_18765"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOS95361.1"
                     /translation="MKVYVLTDMEGVAGVISFDDYVGPEGRYYETGRELTTLEASAAV
                     QGLLDGGATEIHVLDGHGPGAIRPDMLHPAAKLIAGRPLTYPFGLDSTFDAMVIVGQH
                     SKAGTPTGHLCHTGDRLQIDCTLNGRSVGELGLNVAFAGYFGVPCILVTGDQACCDEA
                     RALLPQVETVAVKRGLSQGAAIHLHPDRARALIRDRAWQAISRIGDMAPFQVEPPYEV
                     VSVRPSPDGTLRADWELGAG"
     gene            835..>1626
                     /locus_tag="PLQ54_18770"
     CDS             835..>1626
                     /locus_tag="PLQ54_18770"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOS95362.1"
                     /translation="MGYGLPDGVWLAAFAAWLTALPPLASAGTQQLGACEPYLTEVEP
                     VRVLLADQATRVELSAPGGLVVIDGLGGDGRGVAPVPSCRVETAGAGDERPPAAWMVR
                     IEGLSSEAEARRLADSLVEVTSHAQLANVPGVGPAVLVEGGATPGQCYAILKLIGSRL
                     PDGTAAMVVQSGAGPPGGSLVISRGGEPETVAGPVLVRGVDDAPVEVRATGPGTAVAV
                     AGRFRGTVRIEPSERGGSLELVNLVALEDYLLGVVGCEMPADAPPE"
BASE COUNT          215 a          510 c          631 g          270 t
ORIGIN      
        1 tcgccggccc cgagctccca gtccgcgcgc aacgtgccgt cgggcgacgg gcgcacactg
       61 acgacctcgt agggcggctc cacctggaag ggcgccatgt cgccgatgcg actgatggcc
      121 tgccaggccc ggtcccgaat gagcgcccgt gcgcggtcgg ggtggagatg gatggcggct
      181 ccctgggaca gtccgcgttt gacggccacg gtctccacct gggggaggag cgctcgggcc
      241 tcatcgcagc aagcctggtc accggtcacc aggatgcacg gcacgccgaa gtagccggcg
      301 aacgcgacgt tgaggcccag ttcgccgacc gacctgccgt tgagtgtgca gtcgatctgc
      361 agccggtcgc cggtgtggca cagatggccc gtgggggtgc cggccttgga gtgctggccg
      421 acgatgacca tggcatcgaa tgtggagtcc aggccgaagg ggtatgtgag cgggcggccc
      481 gcgatgagct tggccgccgg gtgaagcatg tccggccgta tggcgccggg gccgtggccg
      541 tcgagcacat ggatctcggt ggctccgccg tcgagcagcc cctgaaccgc ggcgctggct
      601 tcgagggtgg tgagctcgcg gccggtctca tagtagcgtc cctcggggcc gacgtagtcg
      661 tcgaagctga tgacgccggc cacgccctcc atgtcggtca gcacgtatac cttcatcgag
      721 cgactctccc tggcgaagcg gtctcgcgcg ttggttgccg gggtaccaac ctccggttcg
      781 cggcgaacgt ttaacgcatc gccaatcggt cgggaagggg ggagactcga atccatgggg
      841 tatgggctgc ccgatggggt ctggttggcg gcgttcgccg catggctgac ggcgctgccg
      901 ccactggcct ctgcgggcac acagcagctc ggcgcctgtg agccgtatct cactgaagtg
      961 gagcccgtcc gcgtcctgct ggcagaccag gctacccggg tcgagctgtc ggcgcctggc
     1021 gggctggtcg tgatcgatgg cttgggcggc gatggacgcg gggtcgcacc ggttccgtcg
     1081 tgccgggttg agaccgccgg ggccggtgat gagcgaccgc ccgctgcgtg gatggtgcgc
     1141 atcgagggtc tgtcgtccga ggccgaagcc aggcggctgg cggactcgct ggttgaggtg
     1201 acgagtcatg cccagctggc gaacgtgccc ggcgtcggcc ccgcggtctt ggtggagggc
     1261 ggcgcgactc ccggtcagtg ctacgcgatt ctcaagctca ttggctcccg gcttccggac
     1321 ggcactgcgg ccatggtggt ccagagcggt gcaggcccgc ccggcgggag cctggtgatc
     1381 tctcgcggcg gcgagccgga gacggtggcc gggccggtgc tggtgcgcgg ggtcgatgat
     1441 gctccggtgg aggtccgagc cacggggccg gggaccgccg ttgctgtggc cggccggttc
     1501 cgggggaccg ttcggatcga accctcagag cgaggtgggt ccctggagct ggtgaacctg
     1561 gttgcgctcg aggactactt gctcggggta gtcggctgcg agatgccggc ggacgcaccg
     1621 cccgaa
//
LOCUS       DAOSPL010001256         1624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_75746_length_1624_cov_2.718929, whole genome shotgun sequence.
ACCESSION   DAOSPL010001256 DAOSPL010000000
VERSION     DAOSPL010001256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75746_length_1624_cov_2.718929"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="PLQ54_18775"
     CDS             <1..1074
                     /locus_tag="PLQ54_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95363.1"
                     /translation="DSGTKNDDDSLVATRAFRVVELEKLIVFWRGGGAYPFDTDGIDF
                     RALIYVKGSEDYDYRGMETSGEWYKVAHPDTGTPSAEDPEAPASLERSAPPTGKGQES
                     MGVQNWNFLLHDRSDPDDHHNRAYWHTGEGSHSVGGWGANGITLDVGTRNARAYCQLQ
                     DSWGNGTAKVSKYNPSDPAMDGQGVRLSIRTTEYNGHDLTSMLQMAFAYLHVSYEWGG
                     YGFGGKSTANQQTKSGGAIGYDLYGTDCSGFVSAVHWRGGAGWSPWKKTTWDIAADCE
                     LIANHDDPGYGGYDEAEVGDMLNWTEAHVRLIYAITGTGETRRFFYIEASGESLAQGG
                     GRVRTGNCTAAQAKSAHYDIVRG"
BASE COUNT          285 a          511 c          567 g          261 t
ORIGIN      
        1 gactccggca ccaagaacga cgacgactcg ctcgtggcga cgcgggcatt cagggtcgtt
       61 gagcttgaga agctcatcgt cttctggaga ggcggaggtg cgtacccgtt cgacaccgac
      121 gggatcgact tccgggcgct gatatacgtg aagggtagcg aggactacga ctaccgcggc
      181 atggagacca gcggcgagtg gtacaaggtg gcccatcctg acaccggaac gccctccgcg
      241 gaggatccgg aggcgccggc gagcctggaa cggtccgccc cgcccaccgg caaagggcag
      301 gagtcgatgg gcgtccagaa ctggaacttc ctcctgcatg accgatcaga ccccgacgac
      361 caccacaaca gagcctactg gcacacaggc gagggaagcc actctgtcgg cgggtggggc
      421 gcgaatggca tcactctcga cgtgggtacc cgcaacgcac gggcctactg tcagctccaa
      481 gactcgtggg ggaacgggac ggccaaggtc agcaagtaca acccctctga tccagccatg
      541 gatggccagg gggttcgcct atccatcagg accaccgagt acaacgggca tgacctgacc
      601 tcgatgctcc agatggcctt cgcttatctc cacgtttcgt acgagtgggg cgggtatggt
      661 ttcggcggca agagcaccgc caaccagcag accaagtcgg gtggggcgat aggctacgac
      721 ctctacggga cggactgctc cgggttcgtc tctgccgtgc actggagggg cggagcagga
      781 tggagtcctt ggaagaagac cacctgggat atcgcagccg actgtgaact catcgctaat
      841 cacgacgatc cggggtacgg gggctacgac gaggcggagg tcggtgacat gctcaactgg
      901 accgaggccc atgtccgcct catatacgcc atcaccggca ctggagagac caggaggttc
      961 ttctacatcg aggctagcgg cgagtcgctg gcgcaggggg gcggacgggt acgaacgggg
     1021 aactgcaccg ccgcacaggc caagagtgcc cactatgaca tcgtccgtgg gtgagcgctg
     1081 caatgcacca cgccgcgcgg gcgttggtgc ccaggacacc agaaccgggg cgcagatgag
     1141 ccgcccgccc cccggccggc gctatcgtgc gcgccggtac tcggaggagg tggcaggctg
     1201 tgaagcgagc caggttgtgg cttgctgtct gtgtgctcgc cgcgggctac cgattgccgc
     1261 ccccttcggg ggctcgggct cacgtcggga ggagccccga atgggcgttg tgtgcggtag
     1321 cccggggcgg gagccccggg cggcggggct caccgatcgc caccgctctc ccgcgcgccc
     1381 gctgctccgt atgtgttccg cgcgcctgtc gccgcaggcc atctcgcggc ctgcgcgctg
     1441 atactcggag gaggtggcag gctgtgaagc gagccaggtt gtggcttgcc atcagcgtac
     1501 tcgctgttgg catcgtgctt accggtgttc tggtcgctct gccgttggcg atggcggcgc
     1561 cctcgtggga gcccacgacc gcgctgtggg ctccggcgca gttctccgac cccgccctgg
     1621 tagg
//
LOCUS       DAOSPL010001257         1624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_75754_length_1624_cov_2.606119, whole genome shotgun sequence.
ACCESSION   DAOSPL010001257 DAOSPL010000000
VERSION     DAOSPL010001257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75754_length_1624_cov_2.606119"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PLQ54_18780"
     CDS             complement(<1..692)
                     /locus_tag="PLQ54_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95364.1"
                     /translation="MKHWRNFLCVALLAGLPSARAGAQAEGGRQSAAEVEDLLRQVHA
                     VRLLVQMQPTAAQRAALRRKAEDIGPKMRAIEARHDQQLTDAVPALRELLRAYQSGDL
                     ERADRRFDSVRERLEAAEQEAEAGVESLREEAASAYAVLTDIQRCDCFGEFVPERTAR
                     QRVDELSRIPQAERGKSIDEITDALVDQATAEDEAGNVVAPDRAQVRAILLESAALSP
                     AELQARASGFVSR"
     gene            complement(738..>1624)
                     /locus_tag="PLQ54_18785"
     CDS             complement(738..>1624)
                     /locus_tag="PLQ54_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95365.1"
                     /translation="VNLARSATAACREADPGAALVAGLGTLPRPGRGALPLYREVLAL
                     GLGAFADALNVHAYPRDMSPENLARLFSDFSAMANSPALGGERGVQRPIWVTEFGYYA
                     DDDPGPLALPLPLLGSVASEREAASFTVQAMVAMLSQGATRVYLQAFPEWANLSASFT
                     DPLFEWDGQPRKTFAAVAALASLLGPHPRPAGREVVAGVVQAYLFDCGDSAVAAIWPI
                     PLHAPARRVVITPPGPVVPRILDEVGNEIGRPPADGSNAIAVPIDRGQTYVMLAGGDV
                     EDLRVMLRAAALPPSSPD"
BASE COUNT          244 a          546 c          605 g          229 t
ORIGIN      
        1 cggctcacga agcccgacgc ccgggcctga agctcagcgg ggctgagggc cgccgactcg
       61 aggaggatgg cgcgcacctg cgcccggtcg ggcgcaacga cattgccggc ctcgtcctcg
      121 gcggtcgcct ggtcgacgag ggcgtcggtg atctcgtcga tggacttgcc gcgctccgcc
      181 tgggggatcc gcgaaagctc atcgacacgc tggcgggctg tgcgctcggg cacgaactcg
      241 ccgaagcagt cacatcgctg gatgtcggtc aggaccgcat aggcgctggc cgcctcttcg
      301 cggaggctct cgacacccgc ttcggcctcc tgctccgcgg cttccagacg ttcgcggacc
      361 gagtcgaagc ggcgatcggc tcgttcgagg tcgccggact ggtacgcccg caggagctcg
      421 cggagagccg ggacggcatc ggtaagctgt tggtcgtgtc gcgcctcgat ggctctcatt
      481 ttgggtccaa tgtcctcggc cttcctgcgc agggcagctc gctgggcagc cgtgggctgc
      541 atctggacga gcaggcggac cgcatgcacc tggcgcagca ggtcctcgac ctcagcggcc
      601 gactggcggc ccccttcggc ctgcgccccg gcacgggccg aagggagccc ggcgagcagc
      661 gccacacaga ggaagttgcg ccagtgcttc atcgatggtc ctcctcggat tcgctggccg
      721 ccggagcgcc ggcggaccta gtccgggctc gaaggcggaa gggccgcggc gcgcagcatg
      781 acgcgcaggt cctccacatc gcccccggcg agcatcacgt aggtctgccc ccgatcgatg
      841 gggacggcga tggcattgga gccatctgcc ggcggacggc cgatctcgtt gcccacttca
      901 tcgaggattc gagggacgac cggccccggg ggcgtgatga ccacgcgccg ggcgggggca
      961 tggagcggga tgggccagat ggcggcgaca gcggagtcac cgcagtcgaa caggtaggcc
     1021 tggacgacac cggcgaccac ctcgcgcccg gccgggcgcg ggtgagggcc cagcagtgac
     1081 gccagggcag ccaccgctgc gaaggtcttt cgaggctgtc cgtcccactc gaagagcgga
     1141 tccgtgaagg atgcggacag gttcgcccat tcggggaagg cctggaggta gacgcgtgtc
     1201 gcgccctgac tgagcatggc caccatggct tggaccgtga aactggccgc ctcgcgctcc
     1261 gaagcgacac tgccgagcag gggcagcggc agagccagcg gcccgggatc gtcgtcggca
     1321 tagtagccga actcggtgac ccagatcggc cgctgcacgc cgcgctcgcc tccgagcgcc
     1381 gggctgttgg ccatggcgct gaagtcggag aagagccggg ccaggttctc ggggctcatg
     1441 tctctgggat aggcatggac gttgagcgcg tcggcgaagg cccccagtcc gagcgccagg
     1501 acttcgcggt acagggggag tgcgccccgg ccggggcgag gcaaggtgcc gaggcccgcc
     1561 acgagagctg cccccggatc ggcctcgcgg cacgcggccg tggccgagcg ggccaggttc
     1621 acgt
//
LOCUS       DAOSPL010001258         1623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_75809_length_1623_cov_2.998724, whole genome shotgun sequence.
ACCESSION   DAOSPL010001258 DAOSPL010000000
VERSION     DAOSPL010001258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75809_length_1623_cov_2.998724"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="PLQ54_18790"
     CDS             <1..738
                     /locus_tag="PLQ54_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOS95366.1"
                     /translation="VLAAFLGIVTPFCSCSAVPLFIGFVESGVPLGVTLSFLIAAPMI
                     NEVAVVLLFGMVGPQVAGVYVGTGLLIAILCGWIMGRLKLERHVEPWVYEMEAGEALS
                     EGERLRWADRVHAGRQAVREIVGRVWIYVVVGIAVGAGIHGYVPTGTMAAIMGRSAWW
                     SVPVAVVLGVPMYSNAAGIVPIIQALIEKGASLGTALAFMMAVIALSLPEAVILRKVL
                     RPRLLLTYFGVVALGIVFVGYLFNLLW"
     gene            757..990
                     /locus_tag="PLQ54_18795"
     CDS             757..990
                     /locus_tag="PLQ54_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOS95367.1"
                     /translation="MRIQVFGPGCAKCKQLEQNTRAAVQDLGLDVEVEKVEDVREMVK
                     LRILSTPALAVDGQVKSTGKVLSVQEIKQYLSA"
     gene            1003..1401
                     /locus_tag="PLQ54_18800"
     CDS             1003..1401
                     /locus_tag="PLQ54_18800"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HOS95368.1"
                     /translation="MADSCCARTGVRIYACSGGSNVGQLTNEAAKALDGLRQGSMSCA
                     IGVGAGIASFVNDANRPTTVALDGCDVACVRHAFERAGRAPDVHIVVTRLGVEKSHTF
                     DLSDDQIALVAGAAAEAIEAAAATSAEQVG"
     gene            1403..>1623
                     /locus_tag="PLQ54_18805"
     CDS             1403..>1623
                     /locus_tag="PLQ54_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95369.1"
                     /translation="MSGIQTRNLVIIGVVLAAVMAVGLLKAMGPGASTASQDEQTVSP
                     TTTPPGTSGPMGPPIATKAEAKPGAPEEAR"
BASE COUNT          265 a          524 c          550 g          284 t
ORIGIN      
        1 gtcctggccg ccttcctcgg catcgtcact cccttctgct cctgctcggc cgtcccgcta
       61 ttcatcggct tcgtcgagag cggcgtgccg ctcggggtga ccctctcgtt cctcatcgcg
      121 gcgccaatga tcaacgaagt cgcggtagta ctgctgttcg gcatggtcgg cccgcaggtc
      181 gccggggtct acgtgggcac ggggctgctc atcgccattc tctgcggctg gatcatgggt
      241 cgcctcaagc tcgagaggca cgtcgagcca tgggtatacg agatggaggc gggggaggcc
      301 ttgtccgagg gcgaacgcct gcgctgggcc gaccgggtcc acgctggccg gcaggcggtt
      361 cgcgagatcg tgggccgcgt gtggatctac gtggtggtgg gcatcgccgt gggggccggc
      421 attcatggct acgtcccgac cgggaccatg gccgccatca tgggcagatc cgcctggtgg
      481 tccgtgccag tcgccgtcgt gctcggcgtc ccgatgtact cgaacgccgc gggaatcgtg
      541 ccgatcatcc aggccctcat cgagaagggc gcgtccctgg gcacggccct cgcgttcatg
      601 atggccgtca tcgcgctctc gctgcccgag gcggtcattc tccggaaggt attgcgaccc
      661 agactgctac tgacctactt cggcgtggta gcgctcggga ttgtcttcgt gggctatctg
      721 ttcaacctgc tgtggtagca aaaggaggac tcaagaatgc ggatccaggt gttcggaccc
      781 ggttgtgcca agtgcaagca gctcgagcag aacacgcgcg cggcggtcca ggatctcggc
      841 ctcgacgtcg aagtcgagaa ggtcgaggac gtgcgcgaga tggtgaagct ccgcatcctc
      901 agcacgcccg ccctggcggt ggacggccaa gtcaagagca ccggcaaggt gctctcggtc
      961 caagagatca agcagtacct cagcgcttag gggatgagac tcatggcaga ctcgtgctgt
     1021 gcccggactg gcgtgcggat ctacgcctgc tccggaggat cgaacgtggg ccagctcacc
     1081 aatgaagccg caaaggctct ggacggcctc aggcagggca gcatgtcctg tgccatcggc
     1141 gtcggcgccg gcatcgcctc gttcgtgaac gacgccaacc ggcccaccac ggtggccctc
     1201 gatggctgcg atgtggcctg tgtccgtcac gcctttgagc gcgcaggcag ggcaccggac
     1261 gtgcacatcg tcgtgacccg gctgggtgtt gagaagtccc acacattcga cctgagcgat
     1321 gaccagatcg ctctcgtcgc cggcgccgcc gctgaggcca tcgaagccgc tgcggccacg
     1381 tcggcggaac aggtaggttg atatgtccgg cattcagacg cgaaacctcg tcatcatcgg
     1441 cgtggtcctc gcggccgtca tggccgtggg actgctcaag gccatgggac cgggtgccag
     1501 cacggcatcg caagacgagc agaccgtaag ccccacaacg actccgcccg gcacgtcggg
     1561 gcccatgggg ccgcccattg ccaccaaagc cgaggcgaag cccggggcgc ccgaggaagc
     1621 ccg
//
LOCUS       DAOSPL010001259         1621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_75936_length_1621_cov_2.662197, whole genome shotgun sequence.
ACCESSION   DAOSPL010001259 DAOSPL010000000
VERSION     DAOSPL010001259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75936_length_1621_cov_2.662197"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1620)
                     /locus_tag="PLQ54_18810"
     CDS             complement(<1..1620)
                     /locus_tag="PLQ54_18810"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOS95370.1"
                     /translation="MCPENASPSQAHAARELQAFISQMTGAALPVISDAMPLPKCAIL
                     LGHTRYTASILADTNSETRLGDDGFAIRTAGPHLAILGGPIRGTLYGVYELLEHYWGC
                     RWYSTFHSVVPKHDPLTLPEIDDLQVPAFAMREPFWFEMLDGDMAARNRVNGSNMGLE
                     ERHGGKIRFGGGLFVHTFYHLVPPDEFFADHPEYFSLIDGERRVGFSQLCLTNPDVLR
                     IVTERVLERIRRDPSAKLFSVSQNDWDGHCTCDACMEIEQAEESPAGTLIWFVNQVAE
                     AVEREFPDVWIETLAYQYTRKPPKSIRPRHNVVPRICTIECDFSAPLDVSTYPQNQSL
                     VEHMRGWSALSDKLYVWDYTTNFHNYIGPHPNFPALQGNIRFFRDNHVVGLFEQGAAE
                     GPHAEFGELRGWLLAKLLWNPDQPIQPLLDDFFTGYYGAAAGPIRQYFDELQKLPGAA
                     GATLRIFDSLRNRWLNLEFVERAEALFAEAERLASGDDALLYRVRMTGIPVLWARTRL
                     MPEPCRRYRVDGDRLVLCELDPAYRRAGAELL"
BASE COUNT          295 a          469 c          577 g          280 t
ORIGIN      
        1 gagcagttcg gccccggcgc gccggtaggc cggatcgagc tcgcacagca cgaggcggtc
       61 cccgtcgacc cgatagcgcc gacagggctc cggcatcagc cgggtgcggg cccacaagac
      121 cggaatgccg gtcatgcgaa cgcggtagag cagggcatcg tcgccggacg ccagccgctc
      181 ggcctcggcg aagagcgcct cggcccgctc aacgaactcc aggttcagcc accggttgcg
      241 gagcgagtcg aagatgcgga gcgtcgcccc ggccgcgccc gggagcttct ggagctcatc
      301 gaagtactgg cggatggggc cggcggcggc accatagtaa ccagtgaaga agtcgtcgag
      361 caggggttgg atgggctgat ccggattcca caagagcttg gcgagaagcc agccacggag
      421 ctcgccgaac tcggcgtgag gcccttcggc tgccccttgc tcgaagaggc ccacgacgtg
      481 gttatcgcgg aagaagcgga tgttgccctg caatgccggg aagttcgggt gcgggccgat
      541 gtagttgtgg aagttggtgg tgtagtccca gacatagagc ttgtcactga gggcggacca
      601 gccgcgcatg tgttcaacga ggctctggtt ctgagggtat gtgctgacat cgaggggggc
      661 cgagaagtcg cactcgatgg tgcagatccg cggcaccacg ttgtgccggg gccggatgga
      721 cttcggcggc ttgcgtgtgt actgataggc gagggtctca atccagacat cggggaactc
      781 gcgttcgacg gcctcggcta cctggttcac aaaccagatg agggtgccgg cgggggattc
      841 ctcagcctgc tcgatctcca tgcaggcatc gcatgtgcag tgtccgtccc agtcgttctg
      901 gctcacgctg aacagcttgg cgcttggatc gcggcggatg cgttcgagca cgcgctcggt
      961 gacgatgcgc aaaacatcag gattggtgag gcaaagctga gaaaacccga cgcgccgctc
     1021 tccatcgatg aggctgaagt actcggggtg gtccgcgaag aactcgtcgg gcggcacgag
     1081 atgatagaat gtatgcacaa ataggccgcc tccgaaccgg atcttccccc cgtgcctctc
     1141 ctcgagcccc atgttgctgc cgttgacgcg attgcgcgcg gccatgtcgc cgtcgagcat
     1201 ctcgaaccag aagggctcgc gcatggcgaa cgccgggacc tggaggtcgt cgatctcggg
     1261 tagcgtgagc gggtcgtgtt tgggcaccac gctgtggaag gtggagtacc accggcagcc
     1321 ccagtagtgt tcgaggagct catagactcc gtacagcgtg ccgcgaatgg ggccccccag
     1381 gatggccaga tgagggccgg cggtgcggat ggcaaagcca tcgtctccga gccgggtctc
     1441 ggaattggta tctgccagaa tgctggcggt gtaccgggtg tggcccagga gtatggcaca
     1501 cttgggaaga ggcatcgcgt ccgagatcac cggcagggcg gcgcccgtca tctgtgagat
     1561 gaacgcttgg agttcgcggg ccgcatgtgc ctgggatggc gacgcgttct cagggcacat
     1621 g
//
LOCUS       DAOSPL010001260         1620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_75988_length_1620_cov_3.417891, whole genome shotgun sequence.
ACCESSION   DAOSPL010001260 DAOSPL010000000
VERSION     DAOSPL010001260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75988_length_1620_cov_3.417891"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PLQ54_18815"
     CDS             complement(<1..651)
                     /locus_tag="PLQ54_18815"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HOS95371.1"
                     /translation="MAEMRQEAAREGFWDDLEASRAHSQQMSQLERFLAPWQRMERRV
                     AEALEMIELAQTEGDESLVEDVARELEEVEAELAHCEHALLLSGEHDFRNAYLSINAG
                     AGGTESQDWAEMLQRMYTLWAEGRGMEVEVLNCTPGESAGIKSVTCLVKGPFAFGLLR
                     SEVGVHRLVRLSPFDAAHRRHTSFASVDVVPELDDDIHIDISPDDLRIETYRSGGAG"
     gene            complement(778..>1620)
                     /locus_tag="PLQ54_18820"
     CDS             complement(778..>1620)
                     /locus_tag="PLQ54_18820"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOS95372.1"
                     /translation="PIENRLLSRMIENAQRRVESHHFDIRKQVLKFDDVMNTQRSVIY
                     GERRKILEAEDFRDTIMDMIDYVVDRAMERYASPRLSSEEWEMGELLDTLRGVAPIDY
                     LMDTEALAADPPPGDELREQLIGAIRRGYEAREAQFGAEQMRALERWCMLQVIDDQWI
                     DHLAAMDYLRDGIFLRAQGEQKDPVVLYHKEAFDMFEELLEAIREDTIRALFRVRLAE
                     TEEPQQSAYQPQEEGRGSSPGEALPGQRRRAPVRKGQEVGRNDPCPCGSGRKYKRCCL
                     GKAG"
BASE COUNT          267 a          570 c          494 g          289 t
ORIGIN      
        1 ccccgcgccc cccgagcggt acgtctcgat cctgaggtca tcaggagaga tgtcgatgtg
       61 gatgtcatca tccagctcgg gcacgacgtc aaccgaggcg aagctcgtgt gccggcgatg
      121 ggcggcatcg aagggcgaca gccgaaccag ccggtgaacc ccgacctcgg accgcaggag
      181 gccaaaggcg aatggcccct tgaccaggca ggtcacgctc ttgatccccg ccgactcacc
      241 gggggtgcag ttgagaacct cgacctccat gccgcgtccc tcggcccaca gcgtgtacat
      301 gcgctgtagc atctccgccc agtcctggga ctcggtgccg cccgctcccg cgttgatcga
      361 gagatacgcg ttgcggaagt cgtgctcgcc cgagaggagc aatgcatgct cgcagtgggc
      421 gagctcggct tcgacctctt cgagctcgcg agcgacatcc tcgaccaggc tctcgtcccc
      481 ctcggtctgg gcgagctcga tcatctcgag cgcttcggcc acgcgacgct ccattcgctg
      541 ccacggagcc aggaagcgct caagctggct catctgctgc gagtgagcgc gcgaagcctc
      601 caggtcgtcc cagaaaccct ctcgagcggc ctcctggcgc atctcggcca gacgccgctc
      661 cttgccggcg aagtcaaaga cgatccctca cctgaaggag cttgtgacgt atctgctccc
      721 ccaaagcgct gagcgactcc caggtcggag ccgccggtgc ttccgcgctg cccaagacta
      781 ccccgccttt ccgaggcaac accgcttgta cttgcgcccg ctgccacagg gacatgggtc
      841 attccggccg acttcctggc ccttgcgtac cggggcccgc cgtctctgcc cgggcagtgc
      901 ctcgcccggg ctcgagccgc ggccctcttc ctgaggttgg taagcgctct gctgaggctc
      961 ctccgtctcc gcgaggcgga cccggaacag ggcccgaatc gtatcctcgc ggatggcctc
     1021 cagcagctcc tcgaacatgt cgaaggcctc tttgtggtag agcaccacgg gatccttctg
     1081 ctcgccctgg gcacggagga aaatcccgtc tcgcaggtag tccatggcgg ccaggtggtc
     1141 gatccactga tcgtcgatca cctgcagcat gcaccaacgc tccagcgcac gcatctgctc
     1201 ggcgccgaac tgcgcctccc gggcctcgta gccgcgccga atggccccga tcagctgctc
     1261 ccgcagctca tctcctggtg gcgggtcagc cgccagtgcc tcagtatcca tcaggtagtc
     1321 gatgggggcc acgccacgga gagtatccag gagctcaccc atctcccact cctcggagga
     1381 gaggcgcgga ctcgcatacc gctccatggc ccgatcgacg acgtagtcga tcatgtccat
     1441 gatggtgtcg cggaagtcct cggcctcgag gatcttccgg cgctcgccgt agatgacact
     1501 gcgctgggtg ttcatcacat cgtcgaactt gagcacctgc ttgcggatgt cgaagtggtg
     1561 ggactcgacg cggcgctggg cgttctcgat catccgagac agcaggcggt tctcgatggg
//
LOCUS       DAOSPL010001261         1619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_76064_length_1619_cov_2.791560, whole genome shotgun sequence.
ACCESSION   DAOSPL010001261 DAOSPL010000000
VERSION     DAOSPL010001261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76064_length_1619_cov_2.791560"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLQ54_18825"
     CDS             complement(<1..218)
                     /locus_tag="PLQ54_18825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95373.1"
                     /translation="MSQVSQVRMHTTYARVAVYQRDTELDVVHRREPFTSPIRTEVRL
                     ESSATSVEIDGTVMREALGIRTNEAFRR"
     gene            complement(302..>1619)
                     /locus_tag="PLQ54_18830"
     CDS             complement(302..>1619)
                     /locus_tag="PLQ54_18830"
                     /inference="COORDINATES: protein motif:HMM:NF012903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOS95374.1"
                     /translation="IVLAGSQIVGTQVEPRNVFATLIQLRDDLLVADEAYSAPTPTIT
                     GTTDVVRAVQVSPDGTDGTYIRGSLSIQVDSQGRTVAGGVRFSPLYDGLPLDEDQQAQ
                     LDALNAGGVTLASDSLSALGLTLTLQIPPATAGTAVIEIAPVERNMRTQNLNEVGLTA
                     KVSGGGIVAAWGTPLDGRSGSHRLTVSHVALGAATVGGAANVVTGVRNDADQNAGVFR
                     FTTDANGVVLAGSVVFEPAAVGGRAPTAAQQAQLDALNAGGVAVTADALSTFGIRLTT
                     SGGGPPYTPGATIITIAPTTATVTDEFYPADGTGVVTTSDTIMANALNELSNSVRGAT
                     VVTGDLIEGGSAIITSTSRVRELDLNLDVQLRQHADVGARYNRVGANIENLSTNKLNF
                     QSLLSKVEGLDMSAAAVEYQALDNVYEACLSVGSRVILTTLLDYLR"
BASE COUNT          250 a          531 c          540 g          298 t
ORIGIN      
        1 ttccgtctga aggcctcgtt cgtccggatt cccaacgctt cccgcatgac ggttccatcg
       61 atctcgaccg aggtcgccga gctctccagc cgcacctccg tgcggatcgg tgacgtgaac
      121 ggctcgcgcc ggtgcacgac atccagctcg gtgtcacgct ggtagacggc gacgcgggcg
      181 taggtagtgt gcatccgcac ctggctcacc tggctcatgc gtcaccatcc cgttctgccg
      241 atccatgctt ctccgggcct cccgcggcct tcccaccatg gctggggtgt ggcggcaggg
      301 gctatcggag gtagtctagt agcgtcgtca agatcacacg cgatcccacg gagagacatg
      361 cctcgtagac gttgtcgagg gcctggtact cgaccgcggc cgccgacatg tccaggccct
      421 caaccttgct gagcagcgac tggaagttga gcttgttcgt gctcagattc tcgatgttgg
      481 ccccgacgcg gttgtagcgg gctcccacgt cggcgtgctg ccgcagctgg acgtccaggt
      541 tgaggtccag ctcgcgcact cgggaggtcg atgtgatgat cgccgagccc ccctcaatca
      601 ggtcgccggt gacgaccgtg gctccccgga ccgagttcga cagctcgttg agcgcgttgg
      661 ccatgatggt gtcggatgtc gtgacaaccc ccgtcccatc ggcggggtag aactcatcgg
      721 tcacggtggc agttgttggc gcgatggtga tgatcgtggc gccgggggtg tacggggggc
      781 ctccgccgct agtcgtgagg cggataccga aagtgctgag tgcgtcggcg gtcacggcca
      841 cgccccccgc gttgagcgca tcgagctggg cctgctgggc cgccgtcgga gcgcggccgc
      901 cgacggctgc gggctcgaag acgaccgacc cggcgagcac cacaccgttg gcatctgtgg
      961 tgaacctgaa cacacccgcg ttctggtcgg cgtcattgcg gacgccggtc accacgttgg
     1021 ccgctccgcc aaccgtggcg gcccccagtg cgacgtgact cacggtcagc cgatgggacc
     1081 ctgaccggcc gtcgagcggc gtcccccatg ccgccacgat ccccccgccc gagaccttcg
     1141 cggtgagacc aacctcgttg aggttctggg tgcgcatgtt gcgctcgacc ggcgcgatct
     1201 cgatgacggc tgtcccggcc gtggccggcg gaatctggag cgtcagggtg agccccagtg
     1261 cggagaggga atccgatgcc agagttactc cgccggcatt gagtgcatcg agttgcgcct
     1321 gctggtcttc gtcgagaggt aagccgtcgt agagcggact gaagcgcacg ccccccgcca
     1381 cagtccttcc ctggctatcg acctgaatcg acagagagcc tcgaatgtac gttccgtcag
     1441 tcccgtccgg agagacttgc accgctcgga ccacgtcggt tgttcccgtg atggtgggcg
     1501 ttggcgcgga gtacgcctca tcggcgacca gcagatcgtc ccggagctga atgagtgtgg
     1561 caaagacatt ccggggctcg acctgagtcc cgacgatctg ggacccagcg aggacgatc
//
LOCUS       DAOSPL010001262         1616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_76242_length_1616_cov_2.907111, whole genome shotgun sequence.
ACCESSION   DAOSPL010001262 DAOSPL010000000
VERSION     DAOSPL010001262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76242_length_1616_cov_2.907111"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PLQ54_18835"
     CDS             complement(<1..369)
                     /locus_tag="PLQ54_18835"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOS95375.1"
                     /translation="MNCRRALKALPEILDDGGSDALRAAVEEHLHGCPACRREREALS
                     RSWELLDAVRAPAAGPQFTAAVMTRVRASDTRGALPRRATAGPGIRSWWLAPAGAMAL
                     VALLVVSALVTWTHRPPDRPP"
     gene            complement(422..1018)
                     /locus_tag="PLQ54_18840"
     CDS             complement(422..1018)
                     /locus_tag="PLQ54_18840"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS95376.1"
                     /translation="MARLVIDDSELMLRVRSGDHAAFDVLVDRHKQRVLNVVYRYLGP
                     GADAEDIAQEVFIRVYGARRSYVASAKFTTWLYTICRNTCLKEQARRKTRRALEGIPM
                     GGTDHSAEVAADAQTLSPLEAVLLDEHAIVAKRAIDSLPEAQRMALILRKYEGSTYEE
                     IAEAMHCSVRAVKSLLYRARVTLRRELAEYLELGEPSE"
     gene            complement(1205..>1616)
                     /locus_tag="PLQ54_18845"
     CDS             complement(1205..>1616)
                     /locus_tag="PLQ54_18845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOS95377.1"
                     /translation="AARVEHVLHAGDFVAPFAVKPLARLHVPVSAVYGNNDGERQGLA
                     AAFEPFGSVRSRAAVCELGGSRVVVVHEPLLLRQLAHSGDFDLCVYGHTHEVDVRRDG
                     ALIINPGEVCGYVSGRATMVVLDLDTMQPEVIDL"
BASE COUNT          286 a          565 c          520 g          245 t
ORIGIN      
        1 cggcggccga tcgggcgggc ggtgcgtcca ggtcaccagc gcactgacga cgaggagggc
       61 gacgagggcc atcgcccccg ccggggcgag ccaccaacta cggatgccag ggcccgccgt
      121 cgcgcgccgc ggtagcgcgc cgcgcgtgtc gctggcacga acgcgcgtca tgacagcggc
      181 agtgaactgc ggaccggcgg ccggggcccg aaccgcatcc agcagctccc atgagcggct
      241 gagggcctcg cgctcgcgcc ggcacgcggg gcagccgtgc aggtgctctt caacggcggc
      301 acgcagggcg tctgagcccc cgtcatcgag gatctccggc aatgccttca gtgccctccg
      361 gcagttcaac aggtgtccct ccagtgcgca ggcatgaccg gcgtcgaacg gcacgctcca
      421 tctactcgct cggttctccc agctcaaggt attcggcgag ctctcttcgc agtgttacgc
      481 gcgcccggta gagcagcgac ttgaccgctc gaaccgagca gtgcatggcc tcggcgatct
      541 cctcataggt cgagccctcg tacttgcgca ggatcagggc catccgctgc gcctcgggca
      601 gcgagtcgat cgcccgcttc gcgacgattg catgctcgtc cagcaacacc gcctcgagtg
      661 gcgagagggt ctgcgcatcc gccgcgactt cggcggagtg gtccgtaccg cccatcggga
      721 tgccctccag cgccctccgc gtcttgcgcc gggcctgctc cttgaggcac gtattccggc
      781 agatggtgta gagccaagtc gtgaacttgg cacttgccac atatgatctc cgagccccgt
      841 agacccggat gaagacttcc tgggcgatgt cctcggcatc agcccccggc cccaggtagc
      901 ggtagacgac attcagtacc cgctgtttgt ggcgatccac cagcacatcg aacgccgcat
      961 ggtcgcccga acgaacgcgg agcatcagct cggagtcatc gatgaccaga cgcgccatgc
     1021 ttcccctcgg tgccgcccgt cctcatacct cgcatcacca acgccgccac acaccccggc
     1081 ggcccgcggg tgtacggttg aacccgagca gtgcgcccag gtctcggaag acgtgcacga
     1141 agcccaagct atccgatcag ggattcggtg cggtgaccgc cgaacctggt gggacaccgt
     1201 taggctagag gtcgatgacc tcaggttgca tggtgtcgag gtcgagtacc accatggtcg
     1261 cgcggccgct aacgtacccg cagacctcgc cgggattgat gatcagggca ccgtcgcggc
     1321 ggacgtccac ctcgtgcgtg tggccgtaga cacacaggtc gaagtcgccg gagtgagcca
     1381 gctggcgaag cagcaggggc tcgtgcacga cgaccacacg agagcccccc agttcgcaca
     1441 cggccgcacg agaccggaca cttccgaatg gttcgaaggc ggccgccagg ccctgacgct
     1501 caccatcgtt attgccatag accgcagaca cagggacgtg cagtcgcgcc aggggcttca
     1561 cagcgaaggg cgcaacgaag tcgccggcgt gcagaacatg ctccacacgc gcagcg
//
LOCUS       DAOSPL010001263         1615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_76303_length_1615_cov_3.054487, whole genome shotgun sequence.
ACCESSION   DAOSPL010001263 DAOSPL010000000
VERSION     DAOSPL010001263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76303_length_1615_cov_3.054487"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..446
                     /locus_tag="PLQ54_18850"
     CDS             207..446
                     /locus_tag="PLQ54_18850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95378.1"
                     /translation="MWPHTLETFTGSGSSVLTNFGEGTSVTFGSVTFGGATNVFRRDA
                     ILWYHEVRGLKQGNVAFTLHDMKVTRIHIWDPDPS"
     gene            658..1599
                     /gene="dnaG"
                     /locus_tag="PLQ54_18855"
     CDS             658..1599
                     /gene="dnaG"
                     /locus_tag="PLQ54_18855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOS95379.1"
                     /translation="MAHSAERPATAAEIVEAADIVDVVSEYVPLKRAGKDFKAPCPFH
                     SETQPSFYVIPSKRMFYCHGCKAAGNAIQFVQRLLGIPFHEAARRVAQRYGLRYVGRG
                     EPPGQVSVREKLCRVNAFAARFFAQVLSEAPEAAVARDYVARRGISSASVQAFGLGFA
                     PADWHRLRDAAKKRFSEEELVAAGLLVPRETGAAYDRFRGRLMFPITNLEGEVVAFGA
                     RAIGDEEPKYLNSPESAVFAKGKTLYGLAQAREAIRRSGVAVVVEGYTDVIAAHQAGR
                     GNVLATLGTSLTADHVRALSRLAQRVIVAYDADETPI"
BASE COUNT          264 a          501 c          549 g          301 t
ORIGIN      
        1 gccgcgccgg cgcgccggcg ccattcagcg tcgattgcgg cgaagtcaag ggcagcggcc
       61 gctggggcga tgcaaggagc tggtcctata ccctgctgcg tccgctgcaa gccggcgagc
      121 gttgcgattt cgcccggacc gccatgggct gaccccatga gacggtcaag ctgggcgatg
      181 acttcaagcg tgtcctccag cgctacatgt ggccccacac gctcgagaca ttcaccggca
      241 gtggcagttc cgtcctcacc aacttcggtg agggcacttc ggtgaccttc ggttcggtga
      301 ccttcggcgg ggccaccaat gtcttccgcc gtgatgccat cctgtggtac cacgaggtcc
      361 gcgggctcaa gcagggcaat gtggcattca ctctgcacga catgaaggtc acgcggatcc
      421 acatctggga tccggatccc agctagacgg cagcccgccg gtagagactg ctctgcgagg
      481 cccggggttt cgcgcgggcc tcgtgtgttc tctgaggttc cgcctgagga ctctgcggga
      541 gggcgctgag ttcgggtcgg gaacactgct ccgagctggc aaggcccggt ggctcgcgtg
      601 gcggtgtgct gccgatgccg gctatcagcg cggctgcacc caggggatgg agaacccgtg
      661 gctcattccg ccgagagacc tgccactgcg gcggagatcg tcgaggccgc cgacatcgtc
      721 gacgtggtct ccgagtacgt ccctctgaag agggcgggca aggacttcaa agctccatgc
      781 ccgttccact cggagacgca gccgtcgttc tatgtcatac ccagcaagcg gatgttctac
      841 tgccacgggt gcaaggcggc aggcaatgcg atccagttcg tgcaaaggct cctcggaatt
      901 ccattccatg aggcagcccg tcgcgtggca cagcgctatg gtctgcggta tgttggtaga
      961 ggggagccgc ccgggcaggt ttcggttcgc gagaagttgt gtcgagtgaa cgcgttcgcg
     1021 gcccggttct ttgctcaggt gctctctgag gcgcctgagg cggctgtagc gagggactac
     1081 gtggcccggc ggggcatttc ctcagccagc gtgcaggcgt ttggactcgg gttcgcaccg
     1141 gccgactggc atcggctgcg cgatgcggcc aagaagcgat ttagcgagga ggaactggtt
     1201 gccgctgggc tcctggtccc cagggaaacg ggagccgcgt acgatcgctt ccgcggtcgc
     1261 ttgatgttcc ccatcaccaa tctcgaaggt gaagtggtgg ccttcggcgc tcgtgccatc
     1321 ggtgatgagg agcccaagta cctcaacagc cccgagtcgg cagtcttcgc caagggcaaa
     1381 acgctgtacg gattggccca ggcccgcgaa gccattcgcc gctcaggggt tgctgtggtc
     1441 gtggagggct acacggacgt gatcgcggct caccaggccg gccgtggcaa tgtgctggcc
     1501 acgctgggga cctcgctgac ggccgatcac gtacgagccc tgtcccggct ggcccagcgc
     1561 gtgatcgtcg cgtacgacgc cgacgagaca ccgatctaac ctcgtgcttt catga
//
LOCUS       DAOSPL010001264         1615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_76309_length_1615_cov_2.760256, whole genome shotgun sequence.
ACCESSION   DAOSPL010001264 DAOSPL010000000
VERSION     DAOSPL010001264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76309_length_1615_cov_2.760256"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PLQ54_18860"
     CDS             complement(<1..652)
                     /locus_tag="PLQ54_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95380.1"
                     /translation="MDRRHGRAAAALTPCAVLAVAWLGASCGPWAGPNGSANPGSAVR
                     ILVDTRHSHKQNPTISLNPDDYGYSRMHGYSRLFRHLLLSGYGYDLVAQPLTDTLLAR
                     YQILFINLVDDTRPGFEASEIDAIEAFVARGGGLLIITDHSNCYHSAQIINPVLARFA
                     LRATYYTAVERGPDNAIEGGGWLNITRFAAHPVTEGLRRVSFQTGGTIEASSNDATV"
     gene            complement(616..1236)
                     /locus_tag="PLQ54_18865"
     CDS             complement(616..1236)
                     /locus_tag="PLQ54_18865"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOS95381.1"
                     /translation="MAPPAVVAIILAAGGATRLGGRPKPLLPVDGVPMVQRVLAAVRA
                     APSIGRIIVVLGHRADEVAAALAGSPARLVTNPEYRTGRTSSIRCGLRQCPEDVAGVL
                     LLHADQPFVEAGLIETLARAIANERAPAAAVAVGDELATPFAFGAAMLPRLRALADGE
                     GPCMLLRELDSAVARIRVDDPRVVADIDCLEDYRTWTADTGEPRLP"
     gene            1392..>1615
                     /locus_tag="PLQ54_18870"
     CDS             1392..>1615
                     /locus_tag="PLQ54_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015713257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95382.1"
                     /translation="MAGGLVAAATLLSGLALAPTAMAADSATADNAPSVAGHAYNELP
                     YNNPDVTVTQIDNSALPSYMRNPIGQNEGI"
BASE COUNT          255 a          533 c          579 g          248 t
ORIGIN      
        1 gaaccgtggc atcgttcgag gacgcctcga tggtcccgcc cgtctggaaa ctcacccggc
       61 gcagtccctc ggtgaccggg tgcgcggcga agcgtgtgat gttgagccag ccgccgcctt
      121 cgatggcgtt gtcgggcccg cgttcgacgg cggtgtagta cgtggctctg agcgcgaagc
      181 gtgccagcac cgggttgatg atctgggccg agtggtagca gttgctgtgg tcggtgatga
      241 tgagcagccc gccgccgcgc gccacgaagg cctcgatggc gtcgatctcg gaggcttcga
      301 agccgggccg tgtgtcgtcg accaggttga tgaagaggat ctggtagcgc gccagcagtg
      361 tatcggtcag gggttgggcg acgagatcgt agccgtagcc cgagagcagc aggtgccgga
      421 agagccggct gtagccgtgc atcctggagt agccgtaatc gtcggggttc aggctgatgg
      481 tagggttctg cttgtgcgag tgccgtgtgt cgacgaggat ccggaccgcc gagcccgggt
      541 tcgcggagcc gttcgggccc gcccatgggc cgcacgacgc tccgagccat gcaacggcca
      601 ggacggcgca tggagtcagg gcagccgcgg ctcgcccgtg tctgcggtcc acgtgcggta
      661 gtcctccagg cagtcgatgt cggccacgac gcgcggatcg tcgacgcgga ttcgggccac
      721 ggccgagtcg agttcccgca gcagcatgca gggcccctct ccgtcggcca gcgcccggag
      781 ccgagggagc atggccgctc cgaaggcaaa gggcgtggcc agctcgtcgc cgacagcgac
      841 cgcagccgcc ggggctcgct cgttcgcgat cgcgcgcgcc agcgtctcga tgagccccgc
      901 ctcgacgaac ggctggtcgg catgaaggag cagcacgcca gccacatcct ccgggcactg
      961 gcgcaggcca caccggattg acgacgtccg tccggtccgg tactcggggt ttgtgactag
     1021 gcgcgccggg ctgccggcga gagcggcggc gacctcatcg gcccgatggc cgaggacgac
     1081 gatgatgcgc ccgatcgacg gcgcggctcg cacggcggcc aggacgcgct ggaccatggg
     1141 gacaccgtcg acgggcagaa gtggcttggg tcgtccgccg aggcgcgtgg cgccgccagc
     1201 ggcgaggatg atggcgacga cggcgggcgg ggccaaacgc gactcggtcg tgttggggcc
     1261 gcaggcgttc cgaccggccg ggccggacag agtcccacca tcggcctccc ggccgatcgt
     1321 cgcccctgtg tggtccctcc gaagtgccct ccccctcgcg accatgttca cgcgtacacc
     1381 tcgacctgaa ggtggccggt ggactcgtcg ccgcagcgac attgctgtca ggccttgccc
     1441 ttgcgccgac cgcaatggcc gccgattcag ccaccgctga caacgcgccc agcgttgccg
     1501 gtcacgcgta taacgaactg ccgtataaca atcctgatgt caccgtcacc caaatcgaca
     1561 atagcgcact gcccagctac atgcgcaacc ccatcgggca gaacgagggt attga
//
LOCUS       DAOSPL010001265         1611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_76547_length_1611_cov_3.642674, whole genome shotgun sequence.
ACCESSION   DAOSPL010001265 DAOSPL010000000
VERSION     DAOSPL010001265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76547_length_1611_cov_3.642674"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1611
                     /locus_tag="PLQ54_18875"
     CDS             <1..>1611
                     /locus_tag="PLQ54_18875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95383.1"
                     /translation="GPVFEVGGRRYGPDDYAPRVVGQRVVGRRVVTGFRAVLGPVTVG
                     YRLAVWPEGDSLRVRVQSDSPLGRGVTAGRVLCSGAWRRYDLTRTQEAYGQDWWTQTN
                     YLVDEDTFFSAEWMLEGSRASGSEAPDPTDSGRGPFAAAPVLTYTPLTDGTIPAIDET
                     LVLRVGHRLADAMPRPRQRPSEYKAEMARMVYLDLWGGSARDLAHTLALMEAATGGRR
                     PFLTILQPWETGGWDALLPDSIRLPAYGPDPKVGSVEDLRDLADVGKRLGRFGFRTNY
                     MVLRANSPSYIEGRARFALGPDGVPKWHTSPADWAHLAARQEREIRALWRPSASFTDQ
                     LTSGAAPGAWLDFDAAHKPTTGTLGDALERMRGMARAIKEVHHGPLGSESLMDQYLLG
                     EFVDFGDFGIMDGYHRVTCPEYHLRVMRDFTTFFGMGLHYRFVENAPYPEFHKGKCRF
                     WTDRTQQDDYRCMEVLYGNGAYLAYDDGTPWTYVLTELLLVANLQDYYAGARASAVEY
                     LEQGRWQSLEDLIRAGYTYPVSPFGTQPD"
BASE COUNT          248 a          523 c          572 g          268 t
ORIGIN      
        1 gggccggtct tcgaggtcgg tggccggcgc tatgggccgg acgactacgc cccgcgagtt
       61 gtgggccaac gcgtcgtggg gcgccgcgtc gttaccggct tccgcgctgt cctgggaccc
      121 gtgaccgtcg ggtaccgcct cgcagtgtgg ccggaaggcg acagcctgcg cgttcgcgtg
      181 cagtctgact cgccattggg gcgtggtgtt acggcaggcc gagtgctgtg ctccggtgcg
      241 tggcggcggt acgacctgac ccggacacag gaggcctatg ggcaggactg gtggacgcag
      301 accaactacc tggtggacga ggacacgttc ttctccgccg agtggatgct tgaggggagc
      361 cgcgcgtctg ggtccgaggc tcccgaccca acagactccg gccgtggtcc gttcgcggcg
      421 gcgcccgtcc tcacatacac gccgctgacc gatgggacga tcccggcgat cgatgagacg
      481 ctggtccttc gggtcggaca caggctggcc gatgccatgc cacgcccgag gcagcgtccc
      541 agcgagtaca aggccgagat ggcgcgcatg gtgtatctcg acctgtgggg cggctccgcc
      601 cgggacctgg cccacacact ggccctgatg gaggctgcga cgggaggacg tcggcccttc
      661 ctgaccatcc tccagccgtg ggagaccggt ggctgggacg cactgctgcc ggactcaatc
      721 cgtctgccgg cctacgggcc cgaccccaag gtggggtcgg tcgaggatct gcgagacctg
      781 gccgacgtcg ggaaacggct gggccgcttc ggattccgga ccaactacat ggttctccgt
      841 gcgaacagcc ccagctacat cgagggccgg gcccggttcg ctctcgggcc ggatggggtg
      901 cccaagtggc acaccagccc ggcagactgg gcccatctgg cggcacggca ggagcgggag
      961 atccgcgcgc tgtggcgacc atcggcgagt ttcaccgatc aactcacctc aggtgcggcc
     1021 ccgggtgcct ggctcgactt cgacgccgcc cacaagccca cgaccggcac tctgggtgat
     1081 gccctggagc ggatgcgggg catggccagg gccatcaagg aggtgcatca cggtccgctg
     1141 gggtcggagt cgctcatgga ccagtatctt ctgggtgagt tcgtggactt cggcgacttc
     1201 gggatcatgg acggctatca ccgcgtgaca tgccccgagt accatctgcg ggtgatgcgc
     1261 gacttcacga cgttcttcgg catggggttg cactaccggt tcgtcgagaa cgcgccgtac
     1321 cccgagttcc acaagggcaa gtgccggttc tggacggacc gcacgcagca ggatgactac
     1381 cggtgcatgg aggtgctgta cggcaacggc gcgtacttgg cctacgatga cggcacgccc
     1441 tggacgtacg tgctcaccga gttgctgctc gtcgcgaatc tgcaggacta ttacgccggt
     1501 gccagggcct ccgcggtgga gtacctcgag caggggcggt ggcagagcct ggaggacctg
     1561 atccgggcgg gctacaccta cccggtctcg cccttcggca cgcagcccga c
//
LOCUS       DAOSPL010001266         1609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_76657_length_1609_cov_3.203990, whole genome shotgun sequence.
ACCESSION   DAOSPL010001266 DAOSPL010000000
VERSION     DAOSPL010001266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76657_length_1609_cov_3.203990"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="PLQ54_18880"
     CDS             complement(<1..1264)
                     /locus_tag="PLQ54_18880"
                     /inference="COORDINATES: protein motif:HMM:NF020120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HOS95384.1"
                     /translation="MVGIALALAVAGAGRGRAAEPCAIAPQHLACEHLNNPLGIDAAR
                     PRLSWTLRARATERRGLTQQAYQIRVASSPDRLRRGAADLWDTGRVASSECVDVVYAG
                     RPLSSHQPCFWQVRVWDPAGRPSAWSPPAWWTMGLLRPEDWTARWIGHTQPAEQRPGG
                     GGPGGGITFDGAQWIWNSSGDARREVPPGTLCFRRILDLPEGEIARARMAITVDDRFV
                     LDVNGAEVLRTEGSEPWRQPRQVDLAPYLRSGRNVIAIRGTNDALSPAGAMAALSVSV
                     GGREIRLVTDGQWRCGPESPGWESLEFDDSAWAQPVEMGAMGVAPWGALSLGGLTGWG
                     QEQSSPLFRKTVEIGGPLRRATATICGLGYYELRINGEKIGDHVLDPLDRRAVERGAD
                     ELQLAGAGLAVPLGHRPDRAVVLDDAEHP"
     gene            1402..>1609
                     /locus_tag="PLQ54_18885"
     CDS             1402..>1609
                     /locus_tag="PLQ54_18885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95385.1"
                     /translation="MLRLYHRLRDIHPALPQYLLAAALMAVCGGIFENTYNNYLYAEF
                     RISPDARGRLELIREFPGLMNVFLM"
BASE COUNT          233 a          609 c          545 g          222 t
ORIGIN      
        1 cggggtgttc cgcatcgtcg agcacaactg cgcgatctgg gcggtggccg agcggtacgg
       61 ccaggcctgc accagcgagc tggagttcat ccgcaccgcg ctcgacggcg cgacggtcga
      121 gcgggtcgag cacatggtcg ccgatcttct cgccgttgat ccgcagctcg tagtacccga
      181 ggccgcagat ggtggccgtg gcgcggcgaa gcgggccgcc gatctccacc gtcttgcgga
      241 agagcgggct cgactgctcc tggccccagc cggtgaggcc gcccagcgag agcgcgcccc
      301 agggcgccac gcccatggct cccatctcca ccggttgggc ccaagcgctg tcgtcgaact
      361 ccaggctctc ccaaccgggc gactcgggcc cgcagcgcca ctggccgtcg gtgacgaggc
      421 ggatctcgcg ccccccgacg gagacgctca gcgccgccat cgccccggcc ggtgagaggg
      481 catcgttggt cccgcgaatg gcgatgacgt tccgcccgct ccgcaggtag ggggccaggt
      541 cgacctggcg cggctgacgc cacggctcgg agccttcggt ccgcagcacc tcggcaccgt
      601 tgacgtcgag cacgaagcgg tcatcgacgg tgatggccat ccgggcccgg gcgatctcgc
      661 cctcggggag gtccaggatc cggcggaagc agagcgtgcc cggcggaacc tcgcgtcggg
      721 catcgccgga ggagttccag atccactggg cgccgtcgaa ggtgatcccg ccacccgggc
      781 cgccaccgcc cggccgctgc tcggccggct gcgtgtggcc gatccaccga gccgtccagt
      841 cctcgggccg caggaggccc atggtccacc acgcgggggg actccaggcc gatggccgtc
      901 ctgccgggtc ccacacgcgc acctgccaga agcacggctg gtgcgaggac aatggccggc
      961 cggcgtagac gacatcgaca cactcgctgg aggccacgcg gcccgtatcc cacaggtcgg
     1021 cggcgccgcg ccgaagccgg tccgggctcg aggccacgcg gatctgatag gcctgctgcg
     1081 tgagcccgcg gcgctccgtc gcgcgcgccc gaagcgtcca cgagaggcgc ggccgtgcgg
     1141 catcgatccc gagggggttg ttcagatgct cgcacgccag atgctggggc gcgatggcgc
     1201 agggctcggc ggcccggccc cggcccgccc cggcgacggc gagagccagc gcgatgccga
     1261 ccatccacgc ggcccctctc cgccctcccc aagccgcccc gccatgactc acgttctcca
     1321 ttgtgctaca ctcccctgac ctgccgggat gaccggcctc cacggcccga gccgcagact
     1381 acacgatccg gacccaagcc catgctgcgt ctgtaccatc gattgcgaga cattcatccc
     1441 gcactgccgc agtacctgct cgcggcggcg ctcatggccg tctgtggcgg catcttcgag
     1501 aacacctaca acaactatct gtacgccgag ttccgcatct cgcccgacgc ccggggccgg
     1561 ctcgagctca tccgcgagtt ccccggcctc atgaacgtgt tcctgatgg
//
LOCUS       DAOSPL010001267         1608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_76734_length_1608_cov_2.730200, whole genome shotgun sequence.
ACCESSION   DAOSPL010001267 DAOSPL010000000
VERSION     DAOSPL010001267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76734_length_1608_cov_2.730200"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="PLQ54_18890"
     CDS             <1..1335
                     /locus_tag="PLQ54_18890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95386.1"
                     /translation="LVDGEWRPVNGLGGVAEQVRPAPSAEPLHAGDVLLLCTDGLWRE
                     VDDAIMHRILAGSPTPAIASDRLVRAANHSGGRGNIGVVVVHVGRPDIPNDAELDRLF
                     PRPGAPRRDPGTVPMPLASARPVTGGGVSPARPAAPPLAAEGALGPPQPLTGQLPPGP
                     ATPARVIPPPGTSDPSDVAQGTTGPGGMPRRDGLPPGAQNVDPRTIPPRPPMPGVQAS
                     PFMSALGGFAAGVLFLAIVWGVSAKMHNRGASKAAPPAKTQDVTVFYFPKSGAPLTEP
                     DVWARSASSGSGEEWAKLSKGENTLGCEVSPETGEPVVELRANAKLDAQGRVMPMQFG
                     LKIGKVGAGGASSPVRSTSAPGLAPAAAAQGTISVQSTTAGRITVTRQGDREPLQEAD
                     LELDPAGGCSAMLTQLPFGRYVVFHTILRLSQTVTISAEQPSKLVRFSEVDT"
BASE COUNT          228 a          592 c          546 g          242 t
ORIGIN      
        1 cttgtcgacg gcgaatggcg ccccgtgaat ggcttgggcg gcgtggctga acaggtgcgt
       61 cccgccccct cggccgagcc cctgcacgcg ggcgatgtac tcctgctgtg caccgatggg
      121 ctgtggcgcg aggtcgacga cgctatcatg caccgaatcc tcgccggctc ccccaccccg
      181 gccattgcgt cggaccgtct ggtgcgtgcc gccaaccaca gcggcggccg cggcaatatc
      241 ggcgtcgtgg tcgttcatgt gggccgtccg gacatcccga acgacgccga gctcgatcgc
      301 ctgttcccgc gtcccggagc gccgcgccgc gacccgggca ccgtgcccat gcctctcgct
      361 tcggcacggc cggtcacagg cggcggagtc tcgccggctc ggcccgcggc gccgcccctg
      421 gcggccgagg gggccctggg gcccccgcag ccgctcaccg gccaactccc gcccggaccc
      481 gccacgcccg cccgggtgat cccgccccca ggcacgtccg acccctccga cgtggcccaa
      541 ggtaccaccg gcccgggcgg catgccccgc cgcgatgggt tgccgccggg cgcccagaac
      601 gtggacccgc gaaccatccc accccgccca cccatgccgg gtgtgcaggc ctcgccgttc
      661 atgtcggccc tcggtggctt cgcggcaggg gtgctcttcc tggccatcgt gtggggcgtc
      721 tcggccaaga tgcacaaccg cggtgccagt aaggccgccc ccccggccaa gacacaggac
      781 gtcacggtct tctacttccc caagtccggt gcgccattga cggagccgga cgtatgggcc
      841 cggagtgcct cgtccggctc cggtgaggag tgggcgaagc tcagcaaggg cgagaacact
      901 ctcggctgtg aggtctcccc tgagacgggc gagcccgtgg tcgagctgcg cgcgaacgcc
      961 aaattggacg cccagggccg tgtcatgccg atgcagttcg ggctgaagat cggcaaggtc
     1021 ggcgcgggcg gcgcctcctc gcccgtgcgt tccacctcag caccagggct cgcgccggcc
     1081 gcggcggcgc agggcaccat cagcgtgcag agcaccactg cgggacggat caccgtcacg
     1141 cgccagggcg atcgtgagcc gcttcaggag gccgacctgg agctcgaccc tgccggcggg
     1201 tgcagcgcca tgctgacgca gctccccttc ggccgttacg tggtgttcca cacgatcctc
     1261 cggctgtccc agacggtcac catctcggcg gagcagccga gcaagctggt gcggtttagc
     1321 gaagtagaca cttaatgcca gacctcagtg tggtctggtg attcccgcca acaagtggtg
     1381 tagcacgcca cgttcgggtg ctcacgaggc cctcgggtga tcgacgagac tggccaaatc
     1441 gttgcaacga ctcgtcatgt gcgccccggc tctccggccg gagcgctgcg ccttcgaggc
     1501 gggctgcttg ccctcgggcg tgcgggccaa gagagccggc agggggtggc actgtgggcg
     1561 tcctcgagcg tctacgcaac atggttcggg ccaatgtcac cgacatca
//
LOCUS       DAOSPL010001268         1605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_76925_length_1605_cov_3.729677, whole genome shotgun sequence.
ACCESSION   DAOSPL010001268 DAOSPL010000000
VERSION     DAOSPL010001268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76925_length_1605_cov_3.729677"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PLQ54_18895"
     CDS             complement(<1..475)
                     /locus_tag="PLQ54_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOS95387.1"
                     /translation="MTRDDAYALLTEYTKTPSLIGHALAVEAAMRFYAAKLGGDPEEW
                     AAVGLLHDMDYERWPDLEDHPFRGAEIMRDRGVPELWIRAVLSHGDHTGVPRETPMEK
                     ALFAVDELTGFIVACALVRPSRSLADLGAKSVKKKMKDRSFAAKVNREAIEAGAAV"
     gene            complement(472..>1605)
                     /locus_tag="PLQ54_18900"
     CDS             complement(472..>1605)
                     /locus_tag="PLQ54_18900"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS95388.1"
                     /translation="VLAAMAHREPDRTPVFERIIKSPHADALLGRPCAATSFEYRMER
                     LADGAFTDLCLAEARDRVDLAELLGFDMIALGPNGLPPATRPRRLPDGSWQTGDAIAR
                     QSESGWVQYVRADRPVERTDEELAAEMEAHLAMPHVAASPDPEEYVVFREALRLMRER
                     GLDLAVYCSAYAMPVCTLPRHQLEWLHTRPALMRRLYEHYTDQVIDRLRTCAELGADI
                     VGLGGDLANDQGPFISPAHYREFVLPGIQRQAEAVHGLGRWATNTSDGNLWAVLDDFL
                     LTSGVDGYGEIDYVAGMDLGTLKKRYGDRICFIGNLDIRFTLTRGTPEECREHTVECL
                     RKGWGSGGHILMTSNVVHEGVILENYLACLEAYRDYFGIGSRQ"
BASE COUNT          272 a          557 c          501 g          275 t
ORIGIN      
        1 gcactgcggc tccggcttcg atggcttcgc ggttgacctt ggctgcaaac gagcggtcct
       61 tcatcttctt cttcaccgac ttggcaccga gatccgccag actccggctc ggccgcacca
      121 acgcgcaggc cacgatgaag cccgtgagct catccacggc aaacagcgcc ttctccattg
      181 gcgtctcgcg gggcaccccc gtgtggtcac cgtgcgatag taccgctcga atccaaagct
      241 cgggcacccc acggtcccgc atgatctcgg cgccgcggaa cggatggtcc tcgagatccg
      301 gccaccgttc atagtccatg tcgtgaagca ggcccacggc ggcccactcc tccggatctc
      361 ccccgagctt cgccgcatag aagcgcatgg ccgcctccac cgccagtgcg tgcccgatca
      421 ggctcggtgt cttcgtgtac tcggtcagca acgcatatgc atcgtcacgg gtcattgtct
      481 gctcccgatc ccaaagtagt cacggtacgc ttcgaggcac gccaggtagt tctccagtat
      541 gacgccctca tgcaccacgt tgctcgtcat cagaatgtga ccgcccgagc cccaaccttt
      601 gcggaggcac tcgaccgtgt gctcgcggca ctcctcgggc gtgccgcgag tgagggtgaa
      661 acggatatcc aggttgccga tgaagcagat acggtctccg tagcgcttct tgagcgtgcc
      721 cagatccata ccggccacat agtcgatctc cccgtaaccg tcaactccgc tggtgaggag
      781 gaagtcgtcc agcaccgccc acaggttgcc atcggaggtg ttggtggccc accgccccag
      841 cccgtgcacg gcctcggctt gtcgctggat acccggcaga acgaactccc ggtagtgcgc
      901 cggtgagatg aagggccctt ggtcgttggc caggtcgccg cccaggccca cgatgtcggc
      961 gcccagctcg gcacaggttc gcagccggtc aataacctgg tccgtatagt gctcatagag
     1021 ccgccgcatc agcgcgggcc gggtgtggag ccattcgagc tgatgacgcg gcagcgtgca
     1081 tacgggcatg gcataggcgg agcagtagac ggccagatcc aggccccgct cgcgcatcaa
     1141 gcgaagcgcc tcgcggaaca cgacgtactc ttccgggtcc ggcgaggcag ccacgtgagg
     1201 catggccagg tgtgcctcca tctccgcggc cagttcctcg tcggtgcgct cgacgggccg
     1261 gtccgcgcgg acgtactgaa cccagccgct ctccgattgc ctggcgatgg cgtcccccgt
     1321 ctgccagctg ccgtcaggca gacgccgagg ccgcgtggcc ggcggcaggc cgttcggacc
     1381 cagagcgatc atgtcgaaac caaggagctc agccaagtcg actcggtcgc gagcctcggc
     1441 caggcagagg tcagtgaagg cgccgtctgc cagccgctcc atgcggtact cgaacgacgt
     1501 ggccgcgcag ggccgcccga ggagcgcatc ggcatgcggc gacttgatga tccgttcgaa
     1561 gaccggtgtg cggtcgggct ctcggtgcgc catcgccgcc agcac
//
LOCUS       DAOSPL010001269         1603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_77071_length_1603_cov_3.005814, whole genome shotgun sequence.
ACCESSION   DAOSPL010001269 DAOSPL010000000
VERSION     DAOSPL010001269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77071_length_1603_cov_3.005814"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1603
                     /locus_tag="PLQ54_18905"
     CDS             <1..>1603
                     /locus_tag="PLQ54_18905"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOS95389.1"
                     /translation="LVLLALPLWAQAPPPPTAPVTPPAPGAAPGIPGAPQPGGAPGAP
                     GAPATPGTPGGEGAKTEGGEKKEEEKKTPPNYGFSAEELPEDVKAKLDTIMPKGEPTP
                     EGTVSMSFRNAEVADFLQFLSDHTGYIFIPHTDLAGQVTITCPMPVPVDMALRILESW
                     LAVRGYGMSVDPGTKIVRIEPYARARTRRTGVGQGLDLAKIGDGNGYVTQVIQLMNTD
                     AGKVKDLIAPLVDSEAAVLTASSELNAIVITDTADNVRRIVSVVAALEETETTEVQKV
                     EVIPLEYADARVTAQLLTQLFQQPQISQQQIQQMIQRAGGDPSKVKIPGQGLIGLKGQ
                     VKVVYDERTNALVILAAEEKLALIRDIVKEINHSTASEIEYRVFDLAHADAVQTAEVL
                     NELFEQPRGGVSNRRSLLGGFAFGFGFGPRREGAESYGLKENVVTADIRTNQLIVTAS
                     KENMPIFEELVRELDSEKALSGLTKTYKLKNARAENVAETLSNALRGRRTFGGFLGSL
                     FGSRNRQGTPLQQLEDIQVTSETNTN"
BASE COUNT          314 a          513 c          541 g          235 t
ORIGIN      
        1 ctcgtgctgc tggcgctacc gttgtgggcg caagccccgc cgccgcccac ggctccggtc
       61 acgccccccg cgcccggggc ggctccgggc ataccggggg ccccgcagcc gggtggcgca
      121 ccgggagcac cgggagcgcc ggccactccg ggcacgcccg gtggcgaggg tgcgaagacc
      181 gaggggggcg agaagaagga agaagagaag aagacaccgc ccaactatgg gttcagcgcg
      241 gaggagctgc ccgaggacgt caaggccaag ctcgacacga tcatgcccaa aggcgaaccc
      301 acgcccgagg gcaccgtgtc gatgagcttc cgcaatgccg aggtcgccga cttcctgcag
      361 ttcctgtcgg accacacggg ctacatcttc atcccacata cggatctcgc cggtcaggtg
      421 accattacat gcccgatgcc ggttcccgtg gatatggcgc tccggattct cgagtcgtgg
      481 ctggcggttc gcggctatgg gatgtccgtg gatccgggca cgaaaatcgt ccggatcgag
      541 ccctacgcgc gggcccgcac ccggcgtacc ggagtggggc aggggttgga tctggccaag
      601 atcggtgacg gaaacggcta cgtgactcag gtcatacagc tcatgaacac cgatgccggc
      661 aaggtcaagg acctgatcgc tcccctggtc gacagcgagg ccgcggtgct gaccgcctcg
      721 tcggagctca acgccatcgt gatcaccgac acggccgata acgtgcgccg catcgtctcg
      781 gtggtggcag cgctcgagga gacggagacc actgaggtcc agaaggtcga ggtcatcccg
      841 ctggaatacg ccgatgctcg cgtgacggcc cagctcctga cgcagctgtt ccagcagcct
      901 cagatatccc agcagcagat ccaacagatg atccagcggg cgggcggcga cccgtcgaag
      961 gtgaagatcc cggggcaggg actcatcggg ctcaagggcc aggtgaaggt cgtctacgac
     1021 gaacgcacca acgcattggt gatcctggcg gcggaggaga agctggcgct gatccgggac
     1081 atcgtcaagg agatcaatca cagcacggcc tcggagatcg agtaccgcgt gttcgatctc
     1141 gcacacgccg atgcggtgca gacggccgag gtgctcaacg agctcttcga gcagccccgg
     1201 gggggcgtca gcaaccgccg gtcgctgctt gggggattcg ccttcgggtt cggcttcggg
     1261 ccacggcgtg agggagccga gagctacggc ctgaaggaga acgtggtcac ggccgacatc
     1321 cgcaccaacc agctcatcgt cacggcctcg aaggaaaaca tgccgatctt cgaggagctg
     1381 gtgagggagc tggactccga gaaggccctg tccgggctaa ccaagacgta caagctcaag
     1441 aacgcgcgtg ccgagaacgt ggcggagacg ctgagcaatg ctctccgtgg ccggcgaacc
     1501 tttgggggct tcctcggctc cctgttcggc tctcggaacc gccagggcac tcctctgcag
     1561 caactcgagg acatccaggt cacgtcggag accaacacca acc
//
LOCUS       DAOSPL010001270         1603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_77099_length_1603_cov_2.270672, whole genome shotgun sequence.
ACCESSION   DAOSPL010001270 DAOSPL010000000
VERSION     DAOSPL010001270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77099_length_1603_cov_2.270672"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..>1603
                     /locus_tag="PLQ54_18910"
     CDS             139..>1603
                     /locus_tag="PLQ54_18910"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOS95390.1"
                     /translation="MFARPSGALALGLLVVLLLERGSHGQQAPFEVWSVDALEKVLPS
                     AAAPAGRTAAVSFQGARGEYESAQLVVRSDQPIANLRVELSELTGPEGAIPADALAAH
                     FVGFVPIEANTYATPREELVAPAPCELPDPLLPDAEIAVGAGRCQPVWLTLRIPDEAA
                     SGVYAGTVRLLADGGEIEVPVEATVWPFAVPSERHLQFTNWLSADRIAEHHGVTLWSE
                     EFWAVFDRYAQAMGEHRQTMAWAPLSWIAITEEADGAYVCDFSRFDRWVQTCERHGVA
                     ERIEIQQVGGFEKDWSSTTIVLSDVWVTERATGRAKAVPGQVALPRLLPLVEAHLRER
                     GWLDRTLIHIADEPSQHNTRSWSEVADYVHEQAPGIRRIDAIEGPYFAGHLEVMVPKL
                     NHLRHWWPIYARARDEGAELWFYTCCHPTGLYPNRFLDYSLLKTRILHWYNWRFGLAG
                     YLHWGLNWWTADPFRQVTSNGLPPGDCFIIYPGPDGPL"
BASE COUNT          245 a          513 c          550 g          295 t
ORIGIN      
        1 gtgagcagtg ttgacgtccg gatctccatc gctcgggtcc ggcactaggg attcgggttg
       61 gtgtcaggac gcctcgagtg aggggatggt gcggtctccg gcgaagaggc gcgcccatcg
      121 ccttcgggag gtggccccat gtttgcgcga ccatcgggcg ctctggcctt gggtctgctc
      181 gtggttctgc tgctcgagcg tggatctcac gggcagcagg ctccgtttga ggtgtggagt
      241 gttgatgccc tagagaaggt gctgccctcg gctgcggcgc cggctggtag aaccgcggct
      301 gtgtcattcc aaggggcacg gggcgagtac gagtcggctc agctcgtggt gcgatcggac
      361 cagcccatcg ccaaccttcg cgtcgagctc tcggagctca ccggtccgga gggcgcgatc
      421 cccgccgacg cgctcgccgc ccactttgtg gggtttgtcc ccattgaggc caacacatat
      481 gccacgccgc gcgaggagtt ggtcgcgcca gcgccctgcg aactgcccga ccccctgctg
      541 cctgacgcgg agatcgccgt cggggcgggc cgctgccagc ccgtctggct gaccctgcgg
      601 ataccggatg aggccgcgtc cggcgtgtac gcgggaaccg ttcggctcct cgcggacggt
      661 ggtgagatcg aggtgcccgt tgaggccacg gtctggccgt tcgcggtccc ctctgagcgc
      721 cacctgcagt tcaccaactg gctgagcgcc gaccgcatcg ccgaacacca cggcgtgact
      781 ctctggtcgg aggagttctg ggccgtgttc gatcgctacg cgcaggccat gggggagcac
      841 cgtcagacca tggcctgggc tcccctgagt tggatcgcca tcactgagga ggccgatggc
      901 gcatatgtgt gtgacttctc acgcttcgac cgctgggtcc agacgtgcga acgccacggc
      961 gtcgctgagc gtatcgagat ccagcaggta gggggattcg agaaggattg gtcgagcacc
     1021 accatagtgc tgagcgacgt atgggtcacc gagcgcgcca ccgggagggc caaggccgtg
     1081 cccggccagg tcgcgttgcc ccgcctcctc ccgctggtgg aggcgcacct gcgggagcgc
     1141 ggatggctgg accgcaccct gatccacatc gccgacgaac cttcgcagca caacacgaga
     1201 tcgtggagtg aggtcgccga ctatgtccac gagcaggcgc cagggatacg ccgcattgac
     1261 gccatcgagg ggccgtactt tgcggggcac ctcgaggtga tggtgccgaa gctcaaccat
     1321 ctgcgtcact ggtggcccat ctacgcgagg gcccgggacg aaggggcaga gctgtggttc
     1381 tacacgtgct gccacccgac gggcctctac ccgaaccgct ttctggacta ctcgcttctc
     1441 aagactcgaa tcctgcattg gtacaactgg cggttcgggc tggcggggta cctccactgg
     1501 ggcctgaact ggtggacggc ggatccattc aggcaggtca catccaatgg tctcccgcct
     1561 ggcgattgtt tcatcatcta ccctgggccc gacgggcctc tct
//
LOCUS       DAOSPL010001271         1601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_77231_length_1601_cov_2.398448, whole genome shotgun sequence.
ACCESSION   DAOSPL010001271 DAOSPL010000000
VERSION     DAOSPL010001271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77231_length_1601_cov_2.398448"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /locus_tag="PLQ54_18915"
     CDS             complement(<1..1559)
                     /locus_tag="PLQ54_18915"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS95391.1"
                     /translation="MNHEVYGGIYAQMVFGESFQEPPAPAPVAGFTAYDGAWLVAGDG
                     TLHGAAAPGPKLVCDQEWQRPGACGVEVWFADRAEGNAGLVFPITDAGRGPDRMTAYE
                     ISLDPSRGVLVLGKHEQNWRLLAEHPVDIPVGEWVPLTVRRDAKTITVDIAGRRALSF
                     LDPGEPIVRGAVGLRTWNREASFRNLWVEHEGGHSDLPFRQDGSQDTRDQVSGMWRPV
                     RRGTARAAFFLVTDAPYTGSQSQRICFESGSGAVGVVNRGLNRWGMAFLRDHDYEGCL
                     WARCPRSTRLAIEAQTASGEASLARAVVSLPAGDWQRLTFTLSPDTGTESGRLAILLE
                     EPGQVDLGYVSLLPGEWGRFHGLPVRRDVGEALQRQGLTVLRYGGCIVNTDQYRWKSM
                     IGPRDRRQPYRGFWYPYSTNGWGIIDFVDFCRAAGFLAIPAFNMGETPEDMRDFVEYM
                     NGPSYSEWGRRRAADGHPEPYGLRFIQLGNEEAVNEEYFARFRPMAEAIWTADPDITI
                     VVGDFLYSELIA"
BASE COUNT          267 a          569 c          518 g          247 t
ORIGIN      
        1 gcgatcagct cggagtaaag gaagtcgccg accacgatcg tgatgtccgg gtcggcggtc
       61 cagatcgcct ccgccatggg cctgaagcgc gcgaagtact cctcgttgac ggcctcctca
      121 ttcccgagct ggatgaaccg caggccatag ggctcggggt gaccgtcggc ggctcgtcgc
      181 ctcccccact ccgagtagct gggcccgttc atgtactcga cgaagtcgcg catgtcctcg
      241 ggggtctcac ccatgttgaa cgcgggaatg gcgaggaacc ccgcggcgcg gcagaagtcg
      301 acgaagtcga tgatgcccca cccgttagtc gagtaggggt accagaaccc ccgatagggc
      361 tgacgccgat cccgcgggcc gatcatggac ttccaccggt actggtctgt gttcacgatg
      421 cagcccccat agcggagcac ggtcagcccc tgcctctgga gcgcttcgcc cacgtcgcgc
      481 cgcaccggga gcccatggaa gcgtccccat tcgcccggca ggagcgacac gtagcccagg
      541 tcgacctggc cgggctcctc gagcagaatg gccaggcggc ccgattcggt accggtgtcg
      601 ggcgacagag tgaaggtcag ccgctgccag tctcctgcag gcaggctcac aaccgcccgc
      661 gccagagagg cctctccgct ggctgtctgc gcctcgatgg ccagccgcgt cgacctcggg
      721 catcgcgccc agaggcatcc ctcgtagtcg tggtcgcgga ggaaggccat gccccagcgg
      781 ttcagtccgc gattcacaac acccaccgcg cctgagcccg actcgaaaca gatacgctgg
      841 ctctggctgc cggtgtacgg tgcgtcggtg accaggaaga acgccgcacg cgccgtgccc
      901 cggcggacgg ggcgccacat gccgctgacc tggtcacggg tgtcctggga cccgtcctgc
      961 cggaagggta ggtcggagtg ccctccctcg tgctcgaccc acaagttgcg gaaggaggcc
     1021 tctcggttcc acgttctcaa gcccacggcg ccgcgaacga tcggctcacc gggatcgagg
     1081 aacgacagcg cgcggcgccc tgcgatgtcg accgtgatcg tcttcgcgtc tcgccgcacc
     1141 gtgagcggca cccactcgcc gacgggaatg tcgacgggat gctcggcgag gagccgccag
     1201 ttctgttcgt gcttgcccaa gacaagcacg ccccgtgagg gatccagtga gatctcgtac
     1261 gcggtcatcc ggtcgggtcc gcgaccggca tcggtgatgg ggaacaccag gccggcattt
     1321 ccctcggctc ggtccgcgaa ccacacctcg accccgcacg ccccgggccg ctgccactcc
     1381 tggtcacaaa ccagctttgg gccaggcgcg gcggcaccgt gaagcgtccc atccccggcc
     1441 accagccagg ctccgtcgta agcggtgaac ccggccacgg gcgcaggcgc cggcggttcc
     1501 tggaagctct cgccgaacac catctgcgca tagatgccgc cgtagacctc gtggttcacg
     1561 tcctccaggc aagctcccgt catatacggc gagacgtggg c
//
LOCUS       DAOSPL010001272         1600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_77271_length_1600_cov_3.297087, whole genome shotgun sequence.
ACCESSION   DAOSPL010001272 DAOSPL010000000
VERSION     DAOSPL010001272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77271_length_1600_cov_3.297087"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PLQ54_18920"
     CDS             complement(<1..707)
                     /locus_tag="PLQ54_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HOS95392.1"
                     /translation="MGIRESDREILEYRDLMSVPDKFEQGISLRTVVGAFFIGFIMMP
                     GAIYLGLLVGGNLGPAAEWVTIILFTEAARRSFQTVSRQEIYLLYYLAGTLTAFWGGL
                     ALAGGPFAALIWNQYFAQSPAAASFAQQIPDWVVPRAGSEALNHRTFMQPEWVAPIVI
                     LMVGNVLARVNGFTGAYALFRITSDMERLPFPMAPVAAQGATALAESSAKEEGWRWRV
                     FSIGTMIGLSFGLIYVAV"
     gene            complement(792..>1600)
                     /gene="ribF"
                     /locus_tag="PLQ54_18925"
     CDS             complement(792..>1600)
                     /gene="ribF"
                     /locus_tag="PLQ54_18925"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOS95393.1"
                     /translation="KRAGLVSLAVTFDPHPAAVLSGSSNVACLTTTTEKAGLIDECGV
                     EVLAILAFTRELASLTAAQFVERVLVGCLGLRCLVHGPTHRFGRDGAGTPEATRRIAN
                     RLGFEAVCVEPVGVDGEPVSSTRVRQAVSLGRVDAAARMLGRPYAVTGTVVRGFARGR
                     GLGFPTANVSPPPGKLIPAAGIYACRARLESGTAHPGVVYVGQAPTFQGVEATRVECH
                     LLDFDRDLYGQTLRVEFVQHLRGDQTFGAPAELIEQIGRDVARAREVLGG"
BASE COUNT          277 a          570 c          524 g          229 t
ORIGIN      
        1 accgccacat agatcaggcc gaacgagagg ccgatcatgg tcccgatgga gaacacccgc
       61 cagcgccagc cttcctcctt ggccgatgac tccgccaggg ccgtggcccc ctgggcagcc
      121 acgggcgcca tggggaaggg caggcgctcc atgtccgagg tgatgcggaa tagcgcgtag
      181 gctcccgtga acccgttcac gcgcgccagg acgttgccca ccatgaggat gacaatgggc
      241 gcgacccact cgggctgcat gaaggtgcgg tggttgagcg cctccgagcc ggcgcgtggc
      301 accacccagt cggggatctg ctgcgcgaaa ctcgccgccg ccggcgattg cgcgaagtac
      361 tggttccaga tcagcgccgc gaacgggccc ccggccagcg ccaggccgcc ccagaatgcc
      421 gtcagtgtgc ccgccaggta gtagaggagg tagatctcct ggcggctcac ggtctggaat
      481 gaccgccgcg ccgcctccgt gaacaggatg atggtaaccc actcggccgc cgggcccagg
      541 ttaccgccca ccaggaggcc caggtagatg gcccccggca tcatgatgaa cccaatgaag
      601 aacgcgccga cgacggtccg gagggagatg ccctgctcga acttgtccgg aaccgacatc
      661 aggtcccggt actcgaggat ctcccggtca gactcgcgga tccccacggt tctcctcttt
      721 ccgccactcc cgtctcccca ggggagaggg cttggagacg gaaggtgcac gtccccgcgc
      781 cgagcggccc actaacctcc cagaacttcg cgtgctcgcg ccacgtccct gccaatctgc
      841 tcgatcagtt ccgctggcgc gccgaaggtc tggtcgcccc gaaggtgctg cacgaactcg
      901 acgcgtagcg tctgcccgta taggtctcga tcgaagtcca ggagatggca ttcgacccgg
      961 gttgcctcga caccttgaaa ggtgggggcc tggccgacat ataccacgcc cgggtgcgcg
     1021 gtgccgctct caaggcgggc ccggcacgcg tagatgccgg ccgccggtat cagcttgccc
     1081 gggggcggac tcacgttggc ggtggggaag cccaggccac ggcctcgtgc gaagccgcgc
     1141 accaccgtgc cggttactgc gtagggccga cccagcatgc gcgccgcggc gtcgacacgg
     1201 cccagcgaca ccgcctggcg gactcgcgtg ctgctgacgg gctcgccatc cacgcccacg
     1261 ggctcgacgc agaccgcctc gaaccccaac cggttggcga tgcgccgagt ggcctcgggc
     1321 gtccccgccc catcgcgccc aaagcgatgt gtgggaccgt gcaccaggca ccggagaccc
     1381 agacagccca ccaggactcg ctcgacgaac tgggctgccg tcaggctcgc cagctcccgg
     1441 gtgaaggcca ggatggcgag gacctcgacg ccgcattcat cgatcaggcc tgccttctcc
     1501 gtggtcgttg tcaggcacgc cacattgctg gaacccgaga gcacggcggc cggatgggga
     1561 tcgaatgtga cggccaagct caccaggccc gcacgcttca
//
LOCUS       DAOSPL010001273         1600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_77289_length_1600_cov_2.537217, whole genome shotgun sequence.
ACCESSION   DAOSPL010001273 DAOSPL010000000
VERSION     DAOSPL010001273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77289_length_1600_cov_2.537217"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="PLQ54_18930"
     CDS             complement(<1..1321)
                     /locus_tag="PLQ54_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95394.1"
                     /translation="MVSRMGPSVVFSLALAGLCVWAGCGGGPGGGGGGDTTGPLTATV
                     DAADAVTESFHQACDSAPWPGGAEAFVAAAEANAEVVSVSVDETTGDASVRYRSGVVH
                     ILHHCDSPETETGTTLDSLLDSGAVRDLSAHPVTTRWFPSQDTPAATAAASYDENGLY
                     TAIQQACQRVLDSYHVAGYQVPSAVPTATIDWFRNWGNCGVIYFTGHGGWGRWPTDAG
                     DVPIYALQTAERYEAADGPNPAWAEDLAEGRMVFLSVDKTGDHVEDVRAMAVTRRFFE
                     HYCNPMASHGIVWLNSCDSLTYYTDLAASLCDMGTELVLGWRGPAPVRRATAWSLWAL
                     DRMSGLNVTHPLDPPTRPFTADDVWRELDRRGWTRYVSPQGDTSDLEYFRPTSADDER
                     TALCPVISGGVFHPETESSRANVWLNGYFGRTRGQILVDETPLTIAVW"
BASE COUNT          269 a          534 c          549 g          248 t
ORIGIN      
        1 gccagaccgc aattgtcaaa ggtgtttcat caacaagaat ctgcccgcgc gtgcggccga
       61 agtagccgtt cagccaaaca ttggcccggg acgactccgt ttcggggtgg aacacgccgc
      121 cactgatcac ggggcacagc gccgtcctct cgtcatcggc gctggtgggc cggaagtact
      181 cgaggtcaga tgtgtctccc tggggcgaca cgtagcgtgt ccagccgcgg cggtccagct
      241 ctcgccacac gtcgtcggcg gtgaagggac gagtgggcgg gtccaacggg tgcgtcacgt
      301 tgaggccgct cattcggtcg agggcccaca gggaccacgc cgtggcccga cgcaccgggg
      361 cgggccctcg ccaaccgagg accaactcgg tccccatgtc acagagcgac gcggccaggt
      421 cggtgtagta cgtcaggctg tcgcaggagt tgagccagac gattccgtgg cttgccatgg
      481 ggttgcagta gtgctcgaag aaccggcggg tcacggccat tgcgcgcacg tcctcgacat
      541 ggtcaccagt cttgtcgacg gagaggaaca ccatccggcc ctcggccagg tcctcagccc
      601 aggcgggatt gggcccgtcg gcggcctcgt accgctcggc ggtctgcagg gcatagatcg
      661 gcacgtcgcc ggcgtcggtg ggccagcgtc cccatccacc gtgaccggtg aagtagatga
      721 cgccgcagtt gccccagttt cggaaccagt cgatggtggc cgtgggcacg gcactgggta
      781 cctggtaccc ggcgacatgg tatgagtcga gcacacgctg acatgcctgt tggatggcgg
      841 tgtacaggcc gttctcgtcg taggaggcgg ccgcggttgc ggccggcgta tcctgcgagg
      901 ggaaccagcg ggtggttacc gggtgagcag acagatcgcg cacggcgccc gaatcgagca
      961 ggctatccag cgtagtgccc gtctcggtct ccgggctgtc gcaatggtgg agaatgtgga
     1021 ccacgcccga gcggtaacgg accgatgcat cgccggtggt ctcgtcgacg gagacgctca
     1081 cgacctcggc attggcttcg gccgcagcca caaaggcctc ggcgccaccg ggccaaggag
     1141 ccgagtcgca ggcctggtgg aacgactcag tgaccgcgtc ggccgcgtca acggtggcgg
     1201 tcagcgggcc ggtcgtatcg ccgccgcccc caccaccggg gccgccgccg caaccggccc
     1261 acacgcagag gccggcgaga gcgagggaga agacgaccga cggacccatg cgactgacca
     1321 tgtcgacacc gccctctcga cgcacccaca ccctggcatc caacaggacg atggctcacg
     1381 catgcccttc ttcgcgcatc ggcccgggag tcctcgccgc ggcgctcctt gacgtcagca
     1441 ggagacggtg gggacaatga cctgcaagct tcgcacaacc cgggcccagc gcgctcgggc
     1501 attgtgaggt gtgctcgatg gcgcgaacct cgccgttggt gatcggcacc gtactcctct
     1561 ccacgctctg tggcgtcgcc cctacggccc tcccggcgga
//
LOCUS       DAOSPL010001274         1598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_77403_length_1598_cov_3.508101, whole genome shotgun sequence.
ACCESSION   DAOSPL010001274 DAOSPL010000000
VERSION     DAOSPL010001274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77403_length_1598_cov_3.508101"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1598
                     /locus_tag="PLQ54_18935"
     CDS             <1..>1598
                     /locus_tag="PLQ54_18935"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HOS95395.1"
                     /translation="AALANDTTHGIVPGNLLDCRTRAEQVGNALVLSYEAEDCTVEYR
                     YTPRTGRLDDWSVSVDGGEPFSPASGGGIALIGAGDQRVADVTATLGGVEVNDGARTV
                     RAEWTYEGGGQRARVRWTFGIVGKALTVEASSDDAVIGELNLGNVGAVAMRRTIAVPY
                     LAGSLDYLPQEHLFTCRYLDWTYSHASMCPQGQATYDTKTDGTRNPLRERGYVTVSPC
                     VDEVLPNLPSPPSPYRELLGPRIMLDIWGHHGGTYEGDAENLRDLKDNGVDYLAIISH
                     VWQRYGYDVKLPDHLPANPEFGGDEGMKAFGKAANDCGYVWSLHENYIDLYPDAPSYD
                     PSARVLRADGSPSPAWFNVGTGVQSYGLKCTRALGYAKQNAPEIHRRFGTNAAYLDVH
                     TCVPPWHQLDHEATEPMAAMALCKVERDTELFQYMRDTHGGPLFGEGAQHFYWAGRCD
                     GVEAQVDGGENHAPFLNFDLLKIHPQMVNHGMGYYERWFTRGYQHQLGRDAGSPEQFD
                     KYRAQELAYGHAGFIGASQTDNVQ"
BASE COUNT          297 a          541 c          518 g          242 t
ORIGIN      
        1 ggctgcgctg gccaatgaca ccacccatgg catcgtgccg ggcaacctac tggattgccg
       61 gacgcgcgcc gaacaagtgg gcaacgccct ggtcttgagc tatgaggccg aggactgcac
      121 cgtggagtac cgctacacgc cgcgcaccgg ccggctcgat gactggagcg tcagcgtcga
      181 tggaggagaa ccattctcgc cggcctccgg gggaggcatc gccctgatcg gcgccggtga
      241 tcagcgcgtg gccgacgtga cggccacgct tggaggcgtg gaggtcaacg atggcgcgcg
      301 gaccgtgcga gccgagtgga cctacgaggg aggcggtcag cgcgctcgcg tccgatggac
      361 cttcgggatc gtgggcaaag ccctgacagt ggaggcctca tccgacgacg ctgtcatcgg
      421 cgagctcaac ctcggcaacg tgggcgcggt cgccatgagg cggaccatcg cggtgcccta
      481 tcttgccggc agcctcgact acctcccgca agagcacttg ttcacgtgcc gctacctgga
      541 ctggacctac tcgcacgcat cgatgtgccc tcaagggcag gcgacgtacg ataccaagac
      601 ggatggcacc cgcaatccgc tgcgcgagcg cggctacgtc accgtgtcgc cctgcgtgga
      661 cgaagtgctg cccaacctgc ccagtccgcc gtccccgtac cgcgaactgc tcgggcccag
      721 gatcatgctc gatatctggg gccaccatgg gggcacctac gagggcgacg cggagaacct
      781 gcgcgacctc aaggacaacg gagtcgacta cctggccatc atctcgcacg tgtggcaacg
      841 ctatggctac gacgtgaagc tgcccgacca cctgcccgcc aatcctgagt tcggcggcga
      901 cgagggcatg aaggccttcg gcaaggccgc caacgactgc ggctatgtct ggtcgttgca
      961 cgagaactac atcgacctgt accccgacgc accatcatat gatcccagcg cgcgtgtgct
     1021 gcgggccgac ggaagcccct cgcccgcgtg gttcaacgtg ggcaccggcg tacagagcta
     1081 tggcctcaag tgcacccggg ctctgggtta cgcgaagcaa aatgcgcccg agatccaccg
     1141 gcgctttggc acgaatgcgg catacctcga cgtccacacg tgtgtcccgc catggcacca
     1201 gctcgaccac gaggccaccg agcccatggc ggccatggcg ctgtgcaaag tcgagcgcga
     1261 caccgagctg tttcagtaca tgcgcgatac gcacggcggc ccactcttcg gcgagggggc
     1321 ccagcacttc tactgggccg ggcggtgcga cggggtcgag gcccaggtgg atggcgggga
     1381 gaaccacgcg cccttcctca acttcgacct gctcaagatc cacccgcaga tggtgaacca
     1441 cggcatgggc tactacgagc gctggttcac acggggctac cagcatcaac tgggccgcga
     1501 cgccggcagc cccgagcagt tcgacaagta ccgtgcccag gagctggcct acgggcatgc
     1561 tggcttcatc ggcgcgtccc agaccgacaa cgtgcagt
//
LOCUS       DAOSPL010001275         1598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_77433_length_1598_cov_2.465327, whole genome shotgun sequence.
ACCESSION   DAOSPL010001275 DAOSPL010000000
VERSION     DAOSPL010001275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77433_length_1598_cov_2.465327"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PLQ54_18940"
     CDS             <1..734
                     /locus_tag="PLQ54_18940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95396.1"
                     /translation="AQRVPPVGNPDRPTASRPPSTESVPPPSPDDGGPGREGGGGVPG
                     REVAPAVPDGQPVVPGGAPPSVPPTVPTVPGVEEPPTAPPQGSAPPPPTPPVTAAAKG
                     DYPILYVALNQGQSELHLLKAGQDTIVSAGMQDLESWGWSAGATYVWFIARPAAAAGA
                     EPGPKALYVVIAGTPTKVSGDIMVDPRSVVWLPDDSMVLFIPYTPPGPATLYGVTLPD
                     NQLKELATVAGGRISGLDVAPAPSR"
     gene            complement(794..>1598)
                     /locus_tag="PLQ54_18945"
     CDS             complement(794..>1598)
                     /locus_tag="PLQ54_18945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95397.1"
                     /translation="GTQPDAFRRVRVTYDSGLIVVANRSDEPLSVDAGGLALVLPRSG
                     WAAWMPDGTVRAFSALHPDTGRRVDAIHDARRQLRYIDPRGVITEGASVPTAWQAGHV
                     AVELDEQTDRLRVRGRVLPLRLARPPVETIDFGFDAGTDGWLPGEGVLTFGAHDGLLG
                     LRAVTPDPQLLSPELRINGDSVRILVVRLRTSAGEMGQLYFATGDDPAMTEERMFRLS
                     ITPGREFVECRVPVGESQAWRGHTITRLRLDPVHGPESADIEIDRIWVE"
BASE COUNT          250 a          574 c          554 g          220 t
ORIGIN      
        1 acgcccagcg cgtcccgccc gtcggcaacc ccgaccggcc aacagcgagt cgccccccaa
       61 gcaccgagag cgtgccgccg cccagtcccg acgacggcgg tccaggccgt gagggaggcg
      121 gaggtgttcc gggccgcgag gtggccccgg cggtgcccga tggccagccc gtcgtgccgg
      181 gcggagcgcc gccctcggtg cctcccacgg tcccgacggt tccgggcgtc gaggagcctc
      241 ccaccgcacc gccccaggga tcggcgccgc cacctccgac cccgccggta accgcggcgg
      301 ccaaggggga ctatcccatc ctgtacgtcg ctctgaacca gggccaatcg gaactgcatc
      361 tgctcaaagc gggccaggac accatcgtgt cggccggtat gcaggacctg gagtcgtggg
      421 gctggtcggc cggcgcgacc tatgtgtggt tcatcgcccg gccggcggcg gccgcggggg
      481 ccgagccggg acccaaagcc ctttacgtgg tcattgcggg cacacccacc aaggtgagcg
      541 gcgacatcat ggtcgacccc cgcagcgtgg tgtggctgcc ggacgacagc atggtgctct
      601 tcatccccta cacaccgccc gggccagcga cgctttatgg ggtgacactt cccgacaacc
      661 agctcaagga gctagccacg gtggcgggtg gacggatctc gggcctcgac gtagcgccgg
      721 cgccatcacg ctagagcgcg cagtgtggtc atgacccatg gcccgggccc gagccaggcc
      781 cgggccattg ttctcattcg acccagatcc gatcgatctc gatgtcggcc gactccggcc
      841 catgcaccgg gtccagccgg agccgcgtga tggtgtggcc gcgccaggcc tggctctcgc
      901 ccacgggcac gcggcactcg acgaactccc gtcccggtgt gatgctcagg cggaacatgc
      961 gttcctcggt catcgccgga tcatcgccgg tggcgaagta gagctggccc atctcgcccg
     1021 ccgaggtgcg aaggcgcacg acgaggatac gcacggagtc gccgttgatc ctcagctctg
     1081 ggctcagaag ctgcggatcg ggcgtgaccg cccggagacc cagcaggccg tcgtgcgccc
     1141 cgaatgtgag gacgccttct ccgggcagcc acccgtcagt gcccgcgtcg aagccgaaat
     1201 cgatggtctc gacgggcgga cgcgcgaggc gcagcgggag gactctgccg cgcacccgga
     1261 gccgatcggt ctgctcgtcg agctcgacgg ccacatgtcc ggcctgccat gctgtcggga
     1321 ctgaagcgcc ctcggtgatc acaccgcgcg ggtcgatgta gcggagctgg cgtcgggcat
     1381 cgtggatcgc atcgacgcgc cgacccgtgt cggggtggag ggccgagaag gctcggaccg
     1441 tcccatcggg catccacgcc gcccaaccag accgaggcag gaccaatgcc agtcccccgg
     1501 cgtcgacgga aagcggttcg tcggaccggt tcgccacgac gatgagcccg ctgtcgtagg
     1561 tcacacgaac gcgacggaac gcgtcgggct gcgtgccg
//
LOCUS       DAOSPL010001276         1594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_77702_length_1594_cov_2.870695, whole genome shotgun sequence.
ACCESSION   DAOSPL010001276 DAOSPL010000000
VERSION     DAOSPL010001276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77702_length_1594_cov_2.870695"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..698)
                     /locus_tag="PLQ54_18950"
     CDS             complement(72..698)
                     /locus_tag="PLQ54_18950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95398.1"
                     /translation="MPSPRRGAISATDLKQGLEDRYRSDPEYRASVDRQEAERRARRD
                     ELERAERPLVTDLRAAGIPVDSAWDLYKHPELGETAYPLLVKHLRLAYPDRILNGIAR
                     GFTKDVARKHWPELLSIYLTEDRPEACDGLAATLSGCAVRTHYPDLVAILENEALGET
                     RIYFLRPVHRIGNRMCAGAGRTVIESFANDPQLGQEASRILLGRGRND"
     gene            850..1044
                     /locus_tag="PLQ54_18955"
     CDS             850..1044
                     /locus_tag="PLQ54_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95399.1"
                     /translation="MIIVLAFLVIVGIGQTVSQWDRVTASWSSALGAALGVFVPLGAL
                     AWGVRRAMRAADRVKARTAA"
     gene            complement(1053..1370)
                     /locus_tag="PLQ54_18960"
     CDS             complement(1053..1370)
                     /locus_tag="PLQ54_18960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95400.1"
                     /translation="MERKVVKDQLSGASPADGLFKWLALAWAVAFVPLGLLSLAVEWA
                     AEQVSVDAVANNAGALMGIAITGVVWALLRYAATAGAGRPRLIDDIVVLVGAAFSWTL
                     LPG"
BASE COUNT          271 a          510 c          516 g          297 t
ORIGIN      
        1 tcgacgccgt cagaccggta gaacggcgga gtagcgttca ccgcctcgcg gcatgagcgt
       61 gcacatggga cttagtcgtt ccgtccgcgt cccagcagga ttcggctggc ttcctgaccc
      121 aactgagggt cgttcgcgaa actctcgatg accgtccgtc ctgcgccagc gcacattcga
      181 ttgccaatgc ggtgcacggg tcgcaggaag tagatccgcg tctcgcccaa tgcctcgttc
      241 tcgaggatcg cgactaggtc cgggtagtgg gttcggaccg cacagccgct cagcgtggca
      301 gcgagaccgt cacaggcctc tggtcgatcc tcggtgaggt agatgctgag tagttccggc
      361 cagtgttttc gcgcgacgtc cttggtgaac cctcgggcaa tgccgttgag gatccgatcg
      421 gggtacgcga gtcgaaggtg cttcacaagt agcggatacg cggtctcgcc gagttcgggg
      481 tgtttgtaca ggtcccaagc tgagtccacc gggatcccag ccgcccgaag atcggttacc
      541 aacggccgct cggcacgctc cagttcgtcg cgcctggccc gacgttctgc ttcctgacgg
      601 tcaacgcttg cccggtactc cggatcgctg cgataccggt cttcgagccc ttgcttgaga
      661 tcggtcgcgc tgatcgctcc gcggcgtggt gacggcatgg ctggaccata tgccgagcgc
      721 tgctcccaaa cgccctcagc ggcatgtccg gacgttctcc aaggtgggac gcggtggtac
      781 cgctatgctg cccgggtgtt ggagaagggg ctcgccaggg cccgggcgac gccctcggga
      841 acttggctcg tgataatcgt cttggccttt ctcgtgattg tgggcatcgg tcagaccgtc
      901 agccaatggg atcgggtcac cgcgagttgg tcatccgccc ttggcgctgc gctgggcgtg
      961 ttcgtcccct tgggggcact ggcctgggga gtccgccggg ccatgagggc agccgatcga
     1021 gtgaaagcac gcacggcggc atgagcgttc gtctatccag gcagcagagt ccagctaaac
     1081 gcagcgccga cgaggacgac tatgtcgtca atcagccgag gtcgaccagc gccggccgtt
     1141 gcggcgtacc gcaggagggc ccacaccacg ccggtgatgg caatccccat gagtgcaccc
     1201 gcgttgtttg ctacggcgtc tacagacacc tgctcagccg cccactccac ggcgagggac
     1261 aacaagccga gcggaacaaa cgccacggcc cacgccaacg ccagccactt gaacaggcca
     1321 tcagcggggc tcgcgccaga gagctggtcc ttgaccactt tgcgttccat cggccagatc
     1381 tcgcgccccg agagcgcccg gctggccagt aggtataacc caccgttgag gggtgaactc
     1441 atgcgccgag tgcggccacg tgcagcctct ccttaaccac gttgcgcagc gtagtgctag
     1501 ccccacgcga agtcgcagac gggtagatcc caggacgtcc gctcatcggc attatgtggg
     1561 tgatcggtgt caagaggctt cggtgtcctc cagg
//
LOCUS       DAOSPL010001277         1593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_77786_length_1593_cov_2.390767, whole genome shotgun sequence.
ACCESSION   DAOSPL010001277 DAOSPL010000000
VERSION     DAOSPL010001277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77786_length_1593_cov_2.390767"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1441
                     /locus_tag="PLQ54_18965"
     CDS             164..1441
                     /locus_tag="PLQ54_18965"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOS95401.1"
                     /translation="MKTTVIVGTQWGDEGKGRICDYLAGQADYIVRYQGGNNAGHTVV
                     VGTKTFKLHLIPSGILHPGKLSIMADGTLVDPRVLAAELDGLQAEGIDTSGLRISGAA
                     HVIMPYHPVIDQLEEERRGAGKIGTTARGVGPAYADKAARTGIRVYDLLNPDVLRKRV
                     AGALEAKNVLFAQLYGAKPMDPDAVCDEVLAVVPKLAPYVADTRRMLVEAADTDARVL
                     LEGAQATFLDLDYGTYPNVTSSHPVSGGACLGTGISPTALDEVIGVVKAYATRVGSGP
                     FPTELEDRLGDSLRERGHEYGTTTGRPRRCGWLDAVMVRTAARINGLTSLAVTKIDVL
                     DHADPIRICVGYEARGETLRSVPSDVSVFEACTPVYEELPGWCCDTSGVRRLADVPAN
                     TRAFLDRIGELAGVPVRFASFGPERTQLVDLAP"
BASE COUNT          251 a          501 c          551 g          290 t
ORIGIN      
        1 aggcgctggc gatgatggaa catcccaata tcgcgcgcgt ctttgacgct ggcggaccct
       61 cggagctata gtgataggtt ggcgccgggc tcaggtgcgg gtcggttcgc cccttttccg
      121 gggtgcgcct gcacggcccg acggcgctgg ggaagcggga tggatgaaga ccaccgtgat
      181 cgtggggacc cagtggggcg atgagggcaa gggcaggatc tgtgactatc tggccggaca
      241 ggcggactac attgtccgct accagggcgg caacaacgcc ggtcatacgg tggtcgtggg
      301 caccaagacg ttcaaactcc atctcatccc atcgggtatc ctccatccgg gcaaactctc
      361 gatcatggcc gatggtaccc tcgtggaccc gcgcgtgctg gcggcagagc tggacgggct
      421 tcaggccgaa gggatcgaca catccggcct ccgcattagc ggcgcggccc acgtcatcat
      481 gccctatcac cccgtgatcg accagctcga ggaggagcgg cgtggggcag gcaagatcgg
      541 caccaccgct cgcggcgtcg gtccggccta cgcggacaag gccgctcgga ctgggatccg
      601 ggtctacgac ctgttgaacc ccgacgtcct gcgcaaacgg gttgccggtg ctctcgaggc
      661 caagaacgtc ctgttcgcgc agctctacgg tgccaagccg atggacccgg acgccgtctg
      721 tgatgaagtg ctcgcggtgg tgccgaagct ggccccctat gtggcggaca cgcggcggat
      781 gcttgtcgag gcggcggaca cggatgcccg ggtgctcctc gagggcgctc aggccacctt
      841 ccttgatctc gactacggga cgtaccccaa tgtcacatcc tcccacccgg tgtccggggg
      901 ggcgtgcctc ggcacaggga tcagcccaac ggcactcgat gaggtgatcg gcgtggtcaa
      961 ggcctacgcc actcgcgtgg ggtctggtcc gttcccgact gagctcgagg atcgcctggg
     1021 cgactccctg cgcgagcggg gccatgagta tggcactacc acgggccgac cgcgtcgttg
     1081 cggatggctc gatgccgtga tggtgaggac ggctgcacgc atcaacggac tgacgagcct
     1141 cgccgtcacc aagatcgacg ttctggatca tgcggatcca atccggatct gtgtaggcta
     1201 tgaggcccgc ggcgagaccc tccggtcagt gccttctgat gtgtcggtct tcgaggcctg
     1261 cacgcccgtg tatgaggagt tgcccgggtg gtgttgtgac acctcgggcg ttcgcaggct
     1321 cgccgacgtg ccggcgaaca cgcgggcgtt cctcgatcgg atcggcgagt tggcaggcgt
     1381 ccccgtgcgg tttgcttcct tcgggcccga gcggacccag ctcgtggacc tcgccccttg
     1441 acaggggctg gcgaatggcc caacctttta tctcctcgcc ggtctccggg cgggttccga
     1501 tgaacggcga gcggtaccgg cgcgggaccc ggtagctcag tggtagagca gcggactttt
     1561 aatccgttgg tcgtgggttc gagtcctccc cgg
//
LOCUS       DAOSPL010001278         1592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_77841_length_1592_cov_3.025374, whole genome shotgun sequence.
ACCESSION   DAOSPL010001278 DAOSPL010000000
VERSION     DAOSPL010001278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77841_length_1592_cov_3.025374"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="PLQ54_18970"
     CDS             <1..1005
                     /locus_tag="PLQ54_18970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HOS95402.1"
                     /translation="IDTLTANSLDFWLSFSIGMGFAIAIVGVVGIIQTHLHRQRVAER
                     GHDWRKLPATRGDFHMGWTVVLFVLSTIGYIVLCAVLVPDFNIAFFIMFGFFWTPLIS
                     YVTARMVGLSGQFIGFPMVLEATYVLSGYKGVAIWFAPIPYNNYGTSVTRFREFELLG
                     GNFRGLLKAELLSFPILMVCSFIFWEFVWRMAAIPSAQFQYCNEFWPLIARQRALFIT
                     GTTESPELFMQAINATYIGAGLGLGLVAYYALQFLGMPTLLIYGLTRGLGQAPSSVIP
                     ELFGACLGRYYFAKRFGLRRWREYAPVLLAGYACGMGLVSMISIAVALISKSISQTPF
                     "
     gene            1045..1299
                     /locus_tag="PLQ54_18975"
     CDS             1045..1299
                     /locus_tag="PLQ54_18975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95403.1"
                     /translation="MPPHRRALTRYLPAVFVLAVAALWYLVFRCTGLLWWDAPDPYSR
                     KLEPPPPLYTPLPTFEGTDPKLQHLTPEMQRKLKELPAEE"
BASE COUNT          256 a          506 c          506 g          324 t
ORIGIN      
        1 atcgacacgc tcacggccaa ctcccttgac ttctggctga gcttctcgat cggtatgggg
       61 ttcgccatcg ccatcgtggg agtggtcggc atcatccaga cgcacctcca tcgccagcgg
      121 gtggccgaga ggggccacga ctggcggaag ctacccgcca cccgtggaga cttccacatg
      181 ggctggacgg tggtgctgtt cgtcctgtcg accatcggct acatcgtgct gtgcgccgtg
      241 ttggtacccg acttcaacat cgcattcttc atcatgtttg ggttcttctg gaccccgctg
      301 atctcgtacg tcacggcccg catggtaggg ctgagcgggc agttcatcgg ttttcccatg
      361 gtcctggagg ccacgtacgt tctgagcggc tacaaggggg tggccatctg gttcgcaccg
      421 atcccgtaca acaactatgg gacttcggtg acgcgcttcc gcgagttcga gctgttgggc
      481 ggcaacttcc gcggcctgct gaaggcggag ctgctcagct tcccgattct catggtgtgc
      541 agtttcatct tctgggagtt cgtctggcgc atggctgcca tcccgtcggc gcagttccaa
      601 tactgcaatg agttctggcc cctgatcgcc cggcagcgtg cgctgttcat cacgggcacg
      661 acggagagcc cggagctgtt catgcaggcc atcaacgcga cgtacatcgg ggccggcctg
      721 gggcttggac tggtagccta ctatgcactg caattcctgg ggatgccgac cttgctgatc
      781 tacggcctga cgcgcggctt gggccaggcc ccgagttcgg tcatccccga gctcttcggc
      841 gcgtgcctgg ggcggtacta cttcgcgaag cgtttcggac tgaggcgctg gagggagtac
      901 gccccggtgt tgctggctgg ctacgcatgc gggatggggc tcgtgagcat gatctccatc
      961 gcggtggccc tgatctcgaa atcgatcagt cagacgccgt tctagatgcc catgcctgcc
     1021 gagactgcgc cagcccaaga gcccgtgcca ccccaccgtc gcgccctgac tcggtacttg
     1081 cccgcggtgt tcgtgctggc cgtggcggca ttgtggtatc tggtgttccg gtgcaccggc
     1141 ctgctgtggt gggacgctcc cgacccgtac tcccggaagc tggagcctcc gcctccgctc
     1201 tacacccctc tccccacatt cgaaggcacc gacccgaagc tgcagcatct caccccggag
     1261 atgcagagga agctgaagga gctcccggcc gaggagtagg gcatacgggc agcggttcga
     1321 gtcatctcct cgttcatcaa cctgacgcgc gtaagatatt ggttgatgca tcatccccac
     1381 ttgaggcgcg ccccggcacg tccgtactgg aaaggagttc gcaatggcag tctcggcacc
     1441 cacgattcaa gagcacacgt tcgactggcg cgggaagccc ggtgtgcggc atgcgacggt
     1501 aggcctggtg gtgcaccaca cggcagaggc cgcgggcagt tgggatgcgg cccgaatcca
     1561 ccggcaccat cgcgacagca acggctgggc gg
//
LOCUS       DAOSPL010001279         1591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_77907_length_1591_cov_3.289714, whole genome shotgun sequence.
ACCESSION   DAOSPL010001279 DAOSPL010000000
VERSION     DAOSPL010001279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77907_length_1591_cov_3.289714"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="PLQ54_18980"
     CDS             complement(<1..1356)
                     /locus_tag="PLQ54_18980"
                     /inference="COORDINATES: protein motif:HMM:NF036483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HOS95404.1"
                     /translation="MRQTTTHISVRGACEHNLRNIDLDLPHDQLVVFTGVSGSGKSSL
                     IYETLFRESQRRHVASLPLHLRALFGGLTRPKADTLDGLRPAIAVQRTGLRSGRRGTV
                     GAITEVLDFLAVLFARAGEGLEADDVDPRVFLPTSHRGACPACGGTGTRDEVDLGALL
                     PDPRRSISEGAIASTTPTGYVRYSRLTRPGWLVLASVLGFSLDTPWEALTPEQRDAVL
                     YGSPGLRQASSLLSDFGGIVPTIQAAYRRSPTGLTRRFVRTVTCQDCLGTRLNPEALR
                     VRLGGRTMAELQGLTVDACQEFLAGVHFDGSRRRIAEAILPEISLRLGLLVELGLGYL
                     HLDRPVDTLSGGEAQRVRLASQVSSGISGVLYVLDEPSVGLHARDHNRLLGVLRRLRD
                     LGNTLCVAEHDRSTILAADYVVDMGPGPADRGGRVVASGPPSAIAESTESITGAYLRG
                     AR"
BASE COUNT          253 a          594 c          534 g          210 t
ORIGIN      
        1 acgcgcgccg cgcaagtagg cgcccgtgat ggactccgtc gactcggcga tggccgacgg
       61 cggccccgat gccaccactc gcccgcccct gtcggcaggc ccgggcccca tgtccaccac
      121 atagtcggcg gccaaaatcg tcgaacggtc gtgctcggcc acgcagaggg tgttgcccag
      181 gtcacgcagg cggcggagca cgcccagcag ccggttgtgg tcgcgcgcat ggagccccac
      241 cgagggctcg tcgagtacgt acagcacgcc ggagatgccc gacgagacct ggctggccag
      301 gcgtacccgc tgggcttcgc cacccgatag cgtgtcgacg ggccggtcga gatgcaggta
      361 ccccagtccc agctcgacga gcaggcccag gcgcagggag atctccggga ggatcgcctc
      421 ggcgatgcgc cggcgcgagc cgtcgaagtg gaccccggcc aggaactcct ggcacgcgtc
      481 caccgtgagc ccttgcagct cggccatggt cctgccaccc agccgaacgc gtagcgcctc
      541 ggggttgagg cgcgtcccca ggcagtcctg gcaggtcacg gtgcgcacaa accggcgcgt
      601 caggccggtc ggtgagcgtc ggtaggccgc ctgtatggtc ggcacgatcc cgccaaagtc
      661 gctcagcagt gaactcgcct gccgcagccc gggactgccg tacagaacgg cgtcccgctg
      721 ctccggtgtg agcgcctccc agggcgtgtc cagagagaag cccagcaccg acgccaagac
      781 gagccatccg ggtcgcgtca gccggctgta gcggacatag ccggtcggcg tcgtcgacgc
      841 aatggccccc tcgctaatgc tccggcgcgg atcgggcagc agtgcgccca gatccacctc
      901 atcccgggtg cctgtgcccc cacacgccgg acaggcgccg cgatgcgatg tgggcaggaa
      961 cacccgcggg tccacgtcgt ccgcctcgag cccctcgccc gcccgggcga acaggacggc
     1021 caggaagtcg agcacctcgg tgatggcgcc cacggtcccc cgccggcccg accggagacc
     1081 ggtccgctga accgcaatgg cgggccgcag gccgtccagc gtgtcggcct ttggccgggt
     1141 gaggccacca aagagcgccc tcaggtgcag cgggagcgac gccacatggc gccgctggct
     1201 ctcgcggaat agcgtctcgt agatcaggct cgacttgccc gaacccgaca caccggtgaa
     1261 cacgacaagc tgatcgtgcg gcaggtcgag atcgatgttc cgcaggttat gctcgcatgc
     1321 cccgcgcacg ctgatatggg tcgtggtctg ccgcacgctc tgcccttcgc ccgttggccg
     1381 tctgggccgg cgcatggcgg atgtgccgcc atctcgccgg ggtcaactgg ctctccttcc
     1441 gcacacgcca tagccgcacc tccgcccgcc cgcacgaaca cccgatcacc gcagcacacc
     1501 gcgggccgct tgaccggcag gtgcacgcgt gaaccggccc gatcgaacgc ggcaagcctt
     1561 gggctgccgc ccaggacccg ggcccctgac c
//
LOCUS       DAOSPL010001280         1590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_77984_length_1590_cov_2.768078, whole genome shotgun sequence.
ACCESSION   DAOSPL010001280 DAOSPL010000000
VERSION     DAOSPL010001280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77984_length_1590_cov_2.768078"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="PLQ54_18985"
     CDS             <1..1114
                     /locus_tag="PLQ54_18985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95405.1"
                     /translation="DMLKSVQPDIQVIGCIETDWVTIYPERMQGGEKLPVAGTGASGG
                     LNAEQTRVIEDAGLPFVDSARRGPDGAMTLELYMRGGKPQTALWVYPAFDAATGESNY
                     QYRFLMEQVEFLLDEVGLDGYYIDEFSQGWTGGIPTYDHWDGISAELDPHTGRVVRLY
                     TDCSLAGVDARVSLARAAIRRGKVMVANTYATTMDEQSLPINRFSETWGSFDPMTCPD
                     GHEPPVIGSLLRSNLASPIGLGILGQPQLHDTARRIMKAVVTYLRHGMLYYHYFIEDI
                     PEEGPGSGEYGPINHMFPITIVGLHPGWIEGRERILTCVSGTYTWRHHAVPRVHVFDL
                     DGRPVELKPGLREMGETWKVTLALQDWAQIAVIEEP"
BASE COUNT          276 a          525 c          522 g          267 t
ORIGIN      
        1 agacatgctc aagtcggtcc agcccgacat ccaggtcatc ggttgcatcg aaaccgactg
       61 ggtcaccatc tatccggagc ggatgcaggg cggcgagaag cttcccgtgg ccggcaccgg
      121 tgcgagcggc ggcctcaacg ccgagcagac ccgggtcatc gaggatgccg gtcttccctt
      181 cgtggatagc gcccggcgtg gtcccgacgg cgccatgacg ctcgagctct acatgcgtgg
      241 gggcaagccc cagacggccc tgtgggtata cccggccttc gacgccgcga cgggggagag
      301 caactatcag taccggttcc tgatggagca ggtcgagttc ctgctcgacg aggtgggtct
      361 ggacggatac tacatcgacg agttcagcca gggctggacc ggtggcatcc cgacctatga
      421 ccactgggat ggcatcagcg cggagctcga cccccacacc ggccgcgtcg tccgtctgta
      481 caccgactgc agtctggccg gagtcgatgc ccgggtaagt ctggctcgcg ccgcgatccg
      541 gagaggaaag gtgatggtgg ccaacaccta tgccaccacc atggacgaac agagcctacc
      601 cattaaccgc ttctccgaaa cgtggggcag cttcgatccc atgacttgcc ccgacggcca
      661 cgagccgccg gttatcggga gcctgctccg cagcaacctg gcttcgccca tcggactcgg
      721 catcctgggg cagccacaac tccacgacac cgcccggcgg atcatgaaag cagtcgtgac
      781 ctacctccgc cacggcatgc tctactacca ctatttcatc gaggacatcc ctgaggaagg
      841 ccccgggagc ggggagtacg gcccgatcaa ccacatgttc cccattacca ttgtgggact
      901 gcaccccggg tggatcgagg gcagggaacg catactgacg tgtgtctccg gaacctatac
      961 atggcgacac cacgcggtgc cgcgcgtgca cgtcttcgac cttgatggcc gccccgtcga
     1021 actgaagccc ggattgaggg agatgggcga gacctggaag gtgacactgg ctctgcagga
     1081 ctgggcgcag atcgcggtga tcgaggagcc ctgaccttca ccggccagcc gaaccccctg
     1141 ttctcacaaa tcccgggtat cgcccctttg ggcggtgcca agcgagccgt gagcagaggc
     1201 cggcttggta gcgcgtcgtg gagcggattg cgcgggctcc agcatgatgc ccctctcgaa
     1261 cacgcttgca ctcgtcggga tcggcctgtc cgtgggtcct gagttgcgct gggtcccctc
     1321 gcgagaccga aggccctcgc gggtcgttcg accggcccag ggcggtggcc caccgcctac
     1381 ctgacggctg ccatgtggcg ctgatctgag acggcgtccc gtgcgttgag tttcctgata
     1441 caatcactcg gcgtctcgac acgcggtggg cgatccggtg ttcgcggacg caccccggcc
     1501 ggcaggcggg gtgcacgccc gccgcgccgc atgttggccg cccggcccgg cgggcgcgta
     1561 gttgggaaag gggtccttga tggagtcgcg
//
LOCUS       DAOSPL010001281         1589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_78041_length_1589_cov_3.292047, whole genome shotgun sequence.
ACCESSION   DAOSPL010001281 DAOSPL010000000
VERSION     DAOSPL010001281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78041_length_1589_cov_3.292047"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /locus_tag="PLQ54_18990"
     CDS             complement(<1..1568)
                     /locus_tag="PLQ54_18990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95406.1"
                     /translation="MRGFAGTTGSWQSSTAIWELLTEVDIEAGRHTVSLVCPGPCMPH
                     IVALRFESSAPFPAGWRRRPKPIEAAPVAWSGTPEPGRYGYEAYVRPDGFVDAPADYD
                     PVIPYERIPAPAPRAERLLEYLLMGEGRYTVRAEMMRDEMDETWSAHLSVVVSEDRTD
                     TGVLPLDAERIRRMAEHTRFLADQFRNRWGLDLREARDEAGALLAAVERLESTADDEA
                     KWRGMYDLYVAAYRLKNRVALSNPLLSFGKLLFAKRQTYDTSHIYTTYFDGSHRFGGS
                     LAMLSPPRPDGPVEAVLAGLPETGIFRDPDVSWDGARALFSYKADGPSPYRIYEVNLD
                     GSGARQLTDSEYDDIDPCYLPDGRIMFVSTRCRRVVLCHNAFTVSVLYTMSSDGSDVR
                     CASSNTVNDFTPSVLADGSVAYTRWEYVDKQLGNNQSLWVVNPDGTMPRHMTGEHWGP
                     ITFWEPRQVPGSSLLVCTLAPHMPIAVGPIALVDPADVCSSPARFTNITPELPPPSHF
                     GWARRDVGYYCNPYP"
BASE COUNT          291 a          541 c          506 g          251 t
ORIGIN      
        1 gggtagggat tgcagtagta gcccacgtcc cggcgggccc agccaaagtg actcggcggc
       61 gggagctccg gcgtgatgtt cgtgaagcgt gccgggctcg aacacacgtc ggccgggtcc
      121 accagcgcga tgggccccac ggcgatgggc atatgcggag ccaaggtgca tacgagcagc
      181 gagctgccgg gaacctgccg gggctcccag aacgtgatcg gcccccagtg ctcgccggtc
      241 atgtgccgcg gcatggtccc gtcaggattc acaacccaca ggctctggtt gttcccgagc
      301 tgcttatcga catactccca tctcgtgtag gcaaccgaac cgtcagcgag cacgctcgga
      361 gtgaagtcgt tcaccgtgtt cgacgaggca caacgtacgt cactgccatc gctggacata
      421 gtgtagagca ccgacacggt gaacgcgttg tggcacagca ccacccgccg acagcgcgtc
      481 gacacgaaca tgatccggcc gtcgggcaga tagcacgggt cgatgtcgtc atactcgctg
      541 tccgtgagct gccgggcacc tgatccgtcg aggttcacct catagatccg gtacggcgac
      601 ggaccatcgg ccttgtacga gaacagggcg cgcgcgccat cccacgagac gtccgggtcg
      661 cgaaagatgc ccgtctccgg aagcccggcc aacacggcct caaccggccc gtccggccgc
      721 ggcggcgaga gcatggccag gctccccccg aaccggtggc tcccgtcaaa gtacgtggtg
      781 tagatgtggc tcgtgtcata cgtctgccgc ttggcgaaca gcagcttccc gaacgacagt
      841 agcgggttgc tcagtgccac gcggttcttg agtcgatagg cagccacata caggtcgtac
      901 atgccgcgcc acttggcctc gtcatcggcc gtcgactcga ggcgctcgac cgcggccaga
      961 agtgctccgg cctcgtcgcg cgcctccctc aggtccaggc cccaacggtt gcgaaactgg
     1021 tccgcgagga agcgcgtgtg ctcggccatc ctccgaatcc gttcggcgtc aagaggcagc
     1081 acgccggtgt cggtgcggtc ctctgagacc accactgaga gatgggcaga ccaggtctcg
     1141 tccatctcat cacgcatcat ctccgcccgc acggtgtagc gcccctcacc cattagcagg
     1201 tactccagca ggcgctcggc acgcggcgcg ggagccggga tccgctcata cgggatgacc
     1261 ggatcgtagt cggcgggagc atcaacgaag ccatcgggtc gcacataggc ctcatagccg
     1321 taccggccgg gctcaggtgt gccgctccac gccacgggtg cggcctcgat gggcttcggt
     1381 ctgcgcctcc acccggcggg gaacggcgct gaagactcga accgcaacgc cacgatatgc
     1441 ggcatgcagg ggccgggaca gaccagagag accgtatgcc gaccggcctc aatgtcgacc
     1501 tccgtcagga gctcccagat ggccgtactc gactgccaac tgcccgtggt gccggcaaac
     1561 cctcgcacga ggtcgacccc atcgagcct
//
LOCUS       DAOSPL010001282         1588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_78136_length_1588_cov_2.758643, whole genome shotgun sequence.
ACCESSION   DAOSPL010001282 DAOSPL010000000
VERSION     DAOSPL010001282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78136_length_1588_cov_2.758643"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PLQ54_18995"
     CDS             complement(<1..395)
                     /locus_tag="PLQ54_18995"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-(5'-phosphoribosyl)anthranilate isomerase"
                     /protein_id="HOS95407.1"
                     /translation="MTRVKICGLTRAADAETALDAGADALGFVFAKSPRQVPPDLVRS
                     IVADLPPLVATIGVFVDAPPDDVRRVLDECGLSGAQLHGSESEEDIRAVGAGRVIKAF
                     RICHSAELESVRDCPAGLVLIEPYVAGKLG"
     gene            complement(392..1174)
                     /gene="trpC"
                     /locus_tag="PLQ54_19000"
     CDS             complement(392..1174)
                     /gene="trpC"
                     /locus_tag="PLQ54_19000"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOS95408.1"
                     /translation="MILDDIVAHKRDDLARRKLEAPLGALNAPTSLAPPSLAAALAGP
                     GVSLIAELKKASPSKGLLRPDYDPQALARAYQRGGAAALSCLTDERFFQGHADHLEAA
                     RAASGLPVLRKDFTLDAYHVHEARAMGASAVLLIAAILTDAELAQLLGVARGLGLDAL
                     VEAHDVSEARRALDCGAQMLGINNRDLRDFSVDLHTTERILDALPSRPPIVVAESGIH
                     TAADMAYLRQLGVQAALVGEALVTAADPAAKARELVEGGRPL"
     gene            complement(1171..>1588)
                     /locus_tag="PLQ54_19005"
     CDS             complement(1171..>1588)
                     /locus_tag="PLQ54_19005"
                     /inference="COORDINATES: protein motif:HMM:NF012800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOS95409.1"
                     /translation="SLMALVAEVSAALGTERAFVVHGGGMDEITTLGPTTVVEVRDGM
                     TRQYDLCPEDLGLAPATLMDLAGGTPAENADILLRVLSGEPGPRRDVVLLNAAAAIAA
                     ADLASSLAEAVPIAQESIDSGAALAKLEALRRFAAP"
BASE COUNT          241 a          547 c          567 g          233 t
ORIGIN      
        1 ccgagcttgc cggcgacgta gggttcgatg agcaccaggc ccgccgggca atcacggacc
       61 gactcgagtt cggccgagtg gcagatgcgg aaggccttga tgacgcgccc ggcgcccacg
      121 gcgcggatat cctcctcgct ctcagacccg tgcaactggg cgccgctcag gccgcactca
      181 tccagcaccc ggcgcacgtc atcgggcggc gcatcgacga acacgccgat cgtcgcaacc
      241 aacggcggga gatcggccac gatcgaccgc acgaggtcgg gaggaacctg gcgtgggctc
      301 ttggcgaaca cgaagcccag cgcgtccgcg cccgcgtcga gggcagtctc ggcgtccgcg
      361 gctcgggtca ggccgcagat cttcacccgc gtcatagcgg ccggcctccc tcgaccagct
      421 cgcgcgcttt ggcggcgggg tcggcggccg tcaccagcgc ctcgccgacc aaggcggcct
      481 gcacgccgag ctggcgcaga taggccatgt cggccgctgt gtggatgccg ctctcggcca
      541 cgacgatggg cgggcggctg ggcaatgcat cgaggatgcg ctcggtggtg tgtaggtcga
      601 ccgagaagtc gcgcagatcg cggttgttga tgcccagcat ctgggcgccg cagtcgagag
      661 cgcgtctggc ttcggaaaca tcgtgcgctt cgaccagcgc gtccagtccg agaccgcggg
      721 ccacgccgag caactgcgcc aactcggcgt cggtcaggat ggcagcgatg agcagcacgg
      781 ccgaagctcc catggcccgg gcttcgtgca cgtggtaggc gtcgagcgtg aagtccttcc
      841 gcagtaccgg caggccgctg gcggcacgag ccgcctccag gtgatcggca tgcccctgga
      901 agaagcgttc gtcggtcagg cacgagagcg cggcagcccc tccccgctgg taggcccggg
      961 cgagcgcctg cggatcgtag tctggccgca ggagcccctt cgatggcgat gccttcttga
     1021 gttcggcgat cagcgagaca ccggggcccg ccagggccgc ggcgaggctc gggggcgcca
     1081 ggctggtcgg cgcgttgagc gcgccgagcg gggcctccag cttccgccgt gccagatcgt
     1141 ctcgcttgtg agccacaatg tcatcgagga tcacggagcg gcaaacctcc ttagtgcctc
     1201 gagcttggcc agcgcggcgc ccgagtcgat gctctcttgg gctatgggga cggcttcagc
     1261 caggctgctg gcgaggtctg cggcggcgat ggcggccgcc gcgttcagca acacgacatc
     1321 gcgccgaggt ccgggctcgc ccgagagaac gcggagcagg atgtcggcgt tctcggcagg
     1381 agtgcccccc gccaggtcca tcagcgtcgc gggtgccagg cccaggtcct cggggcatag
     1441 gtcgtactgg cgcgtcatgc cgtcgcggac ctcgaccacc gttgtggggc ccagcgtggt
     1501 gatctcgtcc atcccgccac cgtgcaccac gaaggcgcgt tccgtcccga gggccgcact
     1561 gacctcagcc accagagcca tgagcgat
//
LOCUS       DAOSPL010001283         1587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_78189_length_1587_cov_3.182115, whole genome shotgun sequence.
ACCESSION   DAOSPL010001283 DAOSPL010000000
VERSION     DAOSPL010001283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78189_length_1587_cov_3.182115"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PLQ54_19010"
     CDS             <1..635
                     /locus_tag="PLQ54_19010"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOS95410.1"
                     /translation="VTTVVVIVFGVPAAFALTRVGGRAGALADNLLAIPLVLPASSVG
                     LALLVAFQWPPVLALQDAVGFRVAYSQAGIVVAQLVLAMAVGVRAWRAAFESVDRRQE
                     QVARTLGSSPARAFFTVILPRPGMGSPPGSSWPGPVPWRSSVPCSSCAARSVGRRTFC
                     RWRCCWTSTRERRNARWCSASRWPSPVRRPSICPRAARGARGIEPRGGLW"
     gene            666..>1587
                     /locus_tag="PLQ54_19015"
     CDS             666..>1587
                     /locus_tag="PLQ54_19015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS95411.1"
                     /translation="MIAIESLSVSLGRFAMRDLSLTIGDGEYLVLLGPTGTGKTVLLE
                     SLLGLHRPDGGSIAIDGTDVTSLPPERRNLGYVPQDAALFPHMTVEGNLAYGLRARRM
                     DRAETDARVADMMRLLRIGYLAGRMPHGLSAGERQRVALGRALAPKPPVCLLDEPLSA
                     LNESLRIELGRELARIQRETRATFVHICHSFDEASELADRVVLLCDGSVVQVGTVADL
                     LDRPASEFVARFTCTRNLLPGCASPAPEGSVVALDAGPRVVSSDRAEGRVVVAIRPEH
                     VTVADGGADGAAGVDGPNVWPGLVVSIQVRS"
BASE COUNT          193 a          494 c          611 g          289 t
ORIGIN      
        1 cggtgaccac ggtggtggtg atcgtgttcg gggtgccggc agcctttgcg ctgacgagag
       61 ttgggggtag ggcgggggcg ctggcagaca acctgctggc gatccccttg gtgctcccgg
      121 cgtcctcggt gggcctcgcg ctgctggtgg ctttccagtg gccccccgtg ctggcgctgc
      181 aggatgcggt ggggttccgg gtggcctaca gccaagcggg gattgtggtc gcgcagctcg
      241 tgctggccat ggcggtcggg gtgcgggcgt ggcgggcagc gtttgagtcg gtggaccgtc
      301 gccaggagca ggtggcgcgg acgctgggca gcagtccggc ccgggcgttc ttcacggtca
      361 tcctgcccag gcccggcatg ggatcgccac cggggtcgtc ctggcctgga cccgtgccat
      421 ggcggagttc ggtgccgtgc tcatcctgtg cagcgcgatc cgtgggacga cggacattct
      481 gccggtggcg atgctgctgg acgtcaacgc gggagagacg gaacgcgcgt tggtgctcgg
      541 cttcgcgctg gccgtcgcct gtgcggcgcc cgtctatctg tcctcgcgcg gcccgcgggg
      601 cgagagggat cgaacctcgg ggcgggctat ggtgagggcc gcatgatgcg ggagtgtgcg
      661 ctgcaatgat cgccattgag tcgctatcgg tgagcctggg acgcttcgcg atgcgtgacc
      721 tcagcctcac catcggggac ggggagtacc tggtcttgct cggcccaacg ggcaccggca
      781 agacggttct gctcgaatcg ctactgggtc ttcaccggcc cgacgggggc agcattgcca
      841 tcgacggaac ggatgtcacc tccctccctc ccgagcgccg gaacctgggg tatgtgccgc
      901 aggatgccgc gctgttcccg cacatgacgg ttgagggcaa cttggcttac ggtctgagag
      961 cccggcggat ggatcgcgcc gagaccgatg cccgggtggc cgacatgatg cggctgctgc
     1021 gcattgggta tctggccggc cgaatgccac acggcttgag tgccggtgag cgccagcgcg
     1081 tggccttggg gcgcgccttg gcgcccaagc cgccggtgtg cttgctcgac gagcccctga
     1141 gcgcgctgaa cgagagcctg cggatcgagc tcggccgcga gctcgcccgg atccagcgcg
     1201 agacccgggc cacctttgtc cacatctgtc acagcttcga cgaggcctcc gagctcgccg
     1261 accgagtcgt ccttctgtgt gatggctcgg tcgtccaggt gggtacggta gccgacctgc
     1321 tggaccggcc cgccagcgag tttgtggccc gcttcacgtg cacccggaat ctgctgccag
     1381 ggtgcgcgtc accggctccc gagggcagcg tcgttgcgct ggacgccggc ccgcgcgtcg
     1441 ttagctctga ccgggccgag gggcgggtcg tggtggcgat ccggccggag cacgtcaccg
     1501 tggccgacgg tggggctgat ggggcggctg gtgttgatgg gccgaacgtg tggccgggct
     1561 tggtcgtgtc gatccaggtc cgatcgt
//
LOCUS       DAOSPL010001284         1586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_78277_length_1586_cov_2.571522, whole genome shotgun sequence.
ACCESSION   DAOSPL010001284 DAOSPL010000000
VERSION     DAOSPL010001284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78277_length_1586_cov_2.571522"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..353)
                     /locus_tag="PLQ54_19020"
     CDS             complement(84..353)
                     /locus_tag="PLQ54_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017786895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOS95412.1"
                     /translation="MTREDIVERVADEAEITKAQAGRAVKAVLDAITEALRDGDKVSL
                     VGFGTFLVRERQARTGKNPRTGEAVDIPAGRVPAFKPGKALKDAV"
     gene            630..>1586
                     /locus_tag="PLQ54_19025"
     CDS             630..>1586
                     /locus_tag="PLQ54_19025"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS95413.1"
                     /translation="MRADAMRAALPLAGLGLLGVLASPGSSEEGSQNPVRLGLVGLDT
                     SHVVVFTAAFNDPANADHVPGVKVVAAYKGGSRDIESSWSRVEGYAQELHEKWGVELV
                     DSIEELCTKVDGVLIESVDGRPHLDQARPVIAAGLPFFVDKPVAGTLADGIEMARLAQ
                     EKSVPWFGGSSLRWYAGVREAVDPETVGEIIGCDAYSPCTTEPHHPDLFWYGTHGVSI
                     LYAAMGRGCKTVTRIATADTDVVVGVWGDGRIGTYRGTRSGAHDYGATVFGTKAVATA
                     RGVSYRGLLEQIVRFFQTKEPPVPAEELLEELAFMEAADRSKA"
BASE COUNT          249 a          469 c          606 g          262 t
ORIGIN      
        1 gggagtagcg ggaggttcgt tgacggcgga gggcgttggg cgcggcgcga ggcacggtgg
       61 cccgcgccgc ccgacatcat ccgctagacc gcgtctttga gggccttgcc cggcttgaac
      121 gcgggcactc tgcccgccgg gatgtcgacg gcctcgccgg tgcgcgggtt cttgccggtg
      181 cgggcctggc gttcgcggac caggaacgtg ccgaaaccca cgagggagac cttgtctcca
      241 tcgcgcagcg cctcggtgat ggcgtcgaga acggctttga ccgctcgccc ggcctgggct
      301 ttggtgatct ctgcttcgtc ggcgacgcgc tcgacgatat cttctcgggt caacttgtgt
      361 tcactcctta gggtgcggcc gcgccctgga cgcggtgggc gtggcacctt gcgcggcccc
      421 aacggccgtc gttcgggaga agctcccggc gggagcatat tcatagacca gcatacccgc
      481 tcctcctggc cggcccgaat cgcagcgcgg aggtggtcga gagcttagac agggcggagg
      541 caggggaccg gcgaatgcgg cgcgggtcgc cgggcgatcc ggcaccgatg tatgtgacgc
      601 gtgagaacca ctcgatggag gtcgagccga tgagagccga cgcgatgcga gccgcgctgc
      661 cactggccgg tttggggctg ctgggggtcc tggcgagccc aggatcgtct gaggaaggga
      721 gccagaaccc ggtacggctg gggctcgtgg gcctcgacac atcccacgtg gtcgtgttca
      781 ccgcggcgtt caacgacccc gccaacgcgg accacgttcc tggtgtgaag gtggtggctg
      841 cctacaaagg gggtagccgt gacatcgagt caagctggag tcgagtggag gggtacgccc
      901 aggagctgca cgagaagtgg ggcgtcgaac tggtggattc gatcgaggag ctgtgcacga
      961 aggttgacgg ggtgctgatc gagagtgtcg acggacggcc tcacttggac caggcgaggc
     1021 cggtgattgc ggccggactg ccgttcttcg tcgacaagcc cgtggcgggc acactggcgg
     1081 atgggatcga gatggcgcgt ctggcccagg agaagagcgt gccctggttt gggggctcga
     1141 gcctgaggtg gtacgccggt gttcgagagg ccgtcgatcc ggagacggtg ggtgagatca
     1201 tcggctgcga cgcgtactcg ccctgcacca cggagccgca ccacccggac ttgttctggt
     1261 atgggaccca cggcgtctcg atcctctacg cggccatggg gcgcgggtgc aagacggtca
     1321 cgcggatcgc gacggccgac accgacgtgg tggtcggcgt gtggggcgat gggaggatcg
     1381 gcacctatcg gggcacgcgc tccggagcgc acgactatgg cgctaccgtg tttggcacca
     1441 aggctgtcgc cacggcccgg ggcgtgagct accgaggact gctggaacag attgtgaggt
     1501 tcttccagac caaggagccc cccgtaccgg ccgaggagct gctcgaggag ctggcgttca
     1561 tggaggcagc cgaccgcagc aaggcg
//
LOCUS       DAOSPL010001285         1584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_78417_length_1584_cov_2.950294, whole genome shotgun sequence.
ACCESSION   DAOSPL010001285 DAOSPL010000000
VERSION     DAOSPL010001285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78417_length_1584_cov_2.950294"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PLQ54_19030"
     CDS             complement(<1..818)
                     /locus_tag="PLQ54_19030"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOS95414.1"
                     /translation="MTHSRWCALAALSVSAAGAQVTPVAHYPFDDPAALGRDASGMGL
                     EARVNGHSGEGKIGRGLILDGTGGVEIPSLPELQAGHALSFELWLRFDDLAHSYGVVA
                     KEDEYLLRLDPPGEGSTLSLFVRADDTWEPRVRSIVPRPNTWYHVVASWDQAILRLII
                     DGQEYSAARSGTVATTGNPVLVGGASTFGPSPLRGAIDEVRFYRGVVRGADVAAALYG
                     IEERLGTPAVERTRFRFTDGLAGWTTTLGGTATAEAGALHLTTAGPHSTLLSPP"
     gene            complement(861..>1584)
                     /locus_tag="PLQ54_19035"
     CDS             complement(861..>1584)
                     /locus_tag="PLQ54_19035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95415.1"
                     /translation="GRTAAAQFADFPLSQILLEPGPEMAPSDVLYTNCRTEIPGAPDH
                     HLMFPTVWHTGSDSTSVVVASSRNGLHWHYLPGGPVLTTAAFGEFDGGCVFAHPNLLE
                     LPNGDFALPYTGYLFPHKYPRGQWRFSPGYAVWPKGRLVALEALEHGEFATVALRVKG
                     TKLRLNVSTKRGGRVLVEAARGNGEPLPGRDFAHAVPIIGDHQAAPVVWHGQEELGAG
                     AEEAIILRFRLHMAALYAIDFV"
BASE COUNT          255 a          506 c          586 g          237 t
ORIGIN      
        1 aatggcgggc tcagcagggt cgagtgcggt ccggccgtgg tcaagtgcaa cgcgccggct
       61 tcggcagtgg cggttccccc cagtgtcgtg gtccaaccgg ccagcccatc ggtgaagcgg
      121 aagcgtgtcc gttcgacggc tggggtgccg agacgttcct cgatgccgta cagcgccgcg
      181 gccacgtcgg cgccgcgcac gacgccgcgg tagaagcgga cctcgtcgat cgctcccctg
      241 agcggcgagg gcccgaaggt cgacgctccc cccacaagca cggggttgcc ggttgtggcc
      301 acggtgccgg agcgggcggc gctgtactcc tgcccgtcga tgatgaggcg gaggatggcc
      361 tgatcccagg aggcgacgac atggtaccag gtgttcgggc gcgggacgat gcttcgcacg
      421 cgcggttccc aagtgtcatc ggcgcgcacg aagagcgaga gtgtgctgcc ctcgcccggc
      481 ggatcgaggc gcagtaggta ctcgtcctcc ttggcgacga cgccgtagct gtgtgccagg
      541 tcgtcgaacc gcagccagag ctcgaagctg agagcatgac cggcctgcag ctcgggcaag
      601 ctggggatct cgacgccacc ggtgccatcg aggatgagac ctcgaccaat cttgccctca
      661 ccgctgtgcc cgttgacgcg ggcttcgagg cccatgccgg aggcatcccg ccccagcgcg
      721 gccgggtcgt cgaaggggta gtgcgccacc ggcgtgacct gggcaccggc cgccgagacg
      781 ctcagtgccg ccagagcgca ccatcggctg tgggtcatgc ggatcactcc caatgacgtc
      841 gggcgccggc ccggccggtg tcagacgaag tcgatggcgt agagcgccgc catgtgaagt
      901 cggaagcgca ggatgatagc ctcttcggcg ccggcaccca gctcctcctg gccgtgccac
      961 accacggggg cggcctggtg gtcaccgatg atgggcactg cgtgagcgaa gtcccggccg
     1021 ggcagcggct cgccgttgcc gcgggcggcc tcgaccagga ccctaccacc acgcttggtg
     1081 gagacgttga ggcggagctt ggtccctttt acccgcagcg cgacggtggc gaactcgcca
     1141 tgctcaaggg cctcgagggc cacgagccgg cccttgggcc agacggcgta gccgggtgag
     1201 aatcgccact gtccgcgcgg gtacttgtgg ggaaagagat aacccgtgta gggaagggcg
     1261 aaatcgccgt tgggcagctc gagcaggttg gggtgagcga agacgcatcc gccatcgaac
     1321 tcgccgaagg cggccgtcgt cagaacgggc ccgccgggca ggtagtgcca gtggaggccg
     1381 ttccggctcg atgcgacgac cacgctggtc gaatcgctgc ccgtatgcca gacggtcgga
     1441 aacatgaggt gatggtcggg cgctccgggg atctcggtcc ggcagttggt gtagagcaca
     1501 tccgaaggcg ccatctctgg cccgggttcg agcaggatct gagacagggg gaagtcggcg
     1561 aactgggcgg ccgcggtccg gccg
//
LOCUS       DAOSPL010001286         1584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_78418_length_1584_cov_2.891432, whole genome shotgun sequence.
ACCESSION   DAOSPL010001286 DAOSPL010000000
VERSION     DAOSPL010001286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78418_length_1584_cov_2.891432"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(303..>1584)
                     /locus_tag="PLQ54_19040"
     CDS             complement(303..>1584)
                     /locus_tag="PLQ54_19040"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOS95416.1"
                     /translation="SVVPINPVFKAPEIAFILRDSEAKAALVHAALAPTLREAADLAA
                     VEPALLLIGGEVEDALAMEAILDVDHEAFDPPAVAPGDIAAVLYTSGTTGRPKGAMLS
                     HDNLVFDARASVDHFGFSPDDRILVVLPMFHAFAATVGLVIPACIGASIVALERFTPQ
                     GVFDAISDHAVTIFPGVPTMFAALLKAADALQWDFASLRACVSGGAPMPPALMEQFEA
                     KFGVPVCEGDGPTECSPITSVNVLHKPRKLGTVGLPLPGVEVRAVDDHDEPVPCGEIG
                     ELVVRGRNVMVGYLNQPEATAEALRGGWFHTGDLGTVDEDGYVAILDRKKDMIIVGGL
                     NVYPREVEDVLYEHPAIQAAAVIGVLDPLRGERVKAVIVRRPGHELSESDVLAHCRGR
                     LANYKLPKLIEFAESLPLSPTGKVLRRELRDPHG"
BASE COUNT          278 a          527 c          532 g          247 t
ORIGIN      
        1 ctggatccgg cggttctcct ccaggctggc ccgacgcgct tccagccggg ccttgtccgt
       61 cgctctctcc cgcgcgatct ccgccggagt cggctcgtgc gagatctgac gatccattcg
      121 cgaacgggtc agccccaccg gttgaggtcg gatctcggcc atacccaacc cccctcgatc
      181 gacgacagac acgcggaggc gacgagtacg cagccgcccg cccccttacc cttatagcat
      241 gccacattcc cgcatgttcc gcacggctcc gcgtcagcgc cgcgtggcga atggcccgcc
      301 ggctatccgt gcgggtcgcg gagctcgcgg cgcagcactt tgccggtggg ggagagggga
      361 agggactcgg caaactcgat gagcttcggc agcttgtagt tggccagtcg accgcggcag
      421 tgggccagca cgtcgctctc ggagagctcg tggccgggac ggcggacgat cacagccttc
      481 acccgttcgc cgcggagggg gtcaaggacc ccgatgacag cggccgcctg gatggcggga
      541 tgctcataga gaacgtcctc aacctcgcgc ggatacacgt tcagcccgcc gacgatgatc
      601 atatctttct tacggtcgag aatggcgaca tagccatcct catccaccgt ccccaggtca
      661 ccggtgtgga accatccacc ccgaagcgcc tcggccgtgg cctcgggttg gttcagatac
      721 cccaccatca cgttgcggcc gcgaacgacg agctcgccga tctccccaca cgggacgggc
      781 tcatcgtggt catcgacggc acggacctcg acaccgggga gcggcaggcc caccgtgccc
      841 agtttgcggg gcttgtggag gacgttcacc gacgtgatgg gtgagcactc ggtgggcccg
      901 tctccctcgc agaccggtac tccaaacttc gcctcgaact gctccatgag ggctgggggc
      961 ataggcgcgc cgccggacac acatgctcgg aggctggcga agtcccactg aagcgcatcg
     1021 gccgccttca ggagggcagc aaacatggtc ggcaccccgg ggaagatggt caccgcgtgg
     1081 tcggagatgg cgtcgaacac gccctgcggc gtgaagcgct ccagagccac gatggatgcg
     1141 ccgatgcatg cggggatcac aagcccgacg gtggccgcga aggcgtggaa catgggcagc
     1201 acgaccaaga tgcggtcatc gggcgagaac ccgaagtggt cgaccgaggc ccgagcgtcg
     1261 aacaccaggt tatcgtggga cagcatggcg cctttgggac ggcccgtggt ccccgaggtg
     1321 tagaggacgg cggcaatgtc gccaggcgcc acagccggag ggtcgaatgc ttcgtggtcg
     1381 acatcgagaa tggcctccat cgccagggca tcctcgacct cgccaccgat caagaggagc
     1441 gccggctcga cggcggccag gtcggcggcc tcgcgcagtg tcggggccaa cgcggcgtgt
     1501 acgagggcgg ctttggcctc gctatccctc aggatgaaag cgatctcggg tgccttgaac
     1561 acggggttaa tgggcaccac gctg
//
LOCUS       DAOSPL010001287         1584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_78437_length_1584_cov_2.432309, whole genome shotgun sequence.
ACCESSION   DAOSPL010001287 DAOSPL010000000
VERSION     DAOSPL010001287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78437_length_1584_cov_2.432309"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1584
                     /locus_tag="PLQ54_19045"
     CDS             <1..>1584
                     /locus_tag="PLQ54_19045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95417.1"
                     /translation="SWLGNPCADAGIADAQGSVVFSVPDLGRGMKWRLPLRDDVPLTD
                     YRYLSMRYRARGLAAGGDYALCVIGSRGGDAMDYQAIVGAGSLIGNGRWQVVSAELRD
                     VAQAIGVARTLAVQVQSAGPDARLEVADIRFTNERRRRRAADAVTIEPFEGFGDFVPL
                     DLGGTRSVSRAQIERHLGVSDWFEGPRVRVDGIPFELAGGGNDLAATDLHAEESELVL
                     PVGRSCGAIYLLMVARMSGNDDPVYGGGRLESISDVDRLLVTLEYADGSTDEQFPWSV
                     SSGEYRVVRGPQVVRLEANPAKELRSLCVYDGSPQTAYYVAAATASSEVPAARPVALP
                     LARDPGPTDLAPGPVALELKADRELTAATRWMEARIGLEDGIRLVSLRSLDPPLEWLA
                     GGQKPELLRVLVDGQPVAFGPWTWVTAEEAPVLVSHSRTGGPPLSARVEVRVDPLQGM
                     GLVPQITNEGPEDLKVSFELMCVPLAAGDPSAMAYCFAQRGTLIDTRPTQLRMLASGL
                     YPLQFITAFSPGARMGAHL"
BASE COUNT          214 a          519 c          577 g          274 t
ORIGIN      
        1 tctcatggct cggcaatccc tgtgcagatg ccggtatcgc ggacgcccag ggctctgtgg
       61 tcttctcggt gcccgacctc ggccgcggga tgaagtggcg cttgccgctt cgggacgacg
      121 tgcccctgac ggattaccgc tacctgtcga tgcgctaccg cgcccggggc ctcgctgccg
      181 gtggtgacta cgccctgtgc gtcatcggat cgcgtggcgg cgacgccatg gactaccagg
      241 ccatcgttgg cgcggggtcg ctcatcggga acggtcgttg gcaggtggtc tcggccgagc
      301 tgagggatgt ggcccaggcg atcggtgtcg ccaggacgct ggcggtccag gtgcagtctg
      361 ccgggcccga tgcccgcctc gaggtcgccg acatacggtt caccaacgag cgccgcaggc
      421 ggcgcgcggc ggacgcggtc actatcgagc ccttcgaggg cttcggtgac ttcgtgcccc
      481 tcgacctcgg cggcacgcgc agcgtgagcc gcgcgcagat tgagcggcat ctgggggtgt
      541 cggactggtt cgagggcccg cgggttcggg tcgacggcat acccttcgag ctggctggcg
      601 gcggcaatga cctcgccgcg acggacctgc atgccgagga gtccgagttg gtgctccccg
      661 tggggcggtc gtgcggggcc atctacctcc tcatggtagc gcggatgtcc ggtaacgatg
      721 acccggtgta cggcggtggc aggctcgagt cgatctctga tgtggaccgc cttctggtga
      781 ctctggagta tgcagacggc tcgaccgacg agcagttccc gtggtcggtc tcaagcggcg
      841 agtaccgcgt ggtgcggggg ccgcaggtcg tgcggctcga ggccaacccg gccaaggagc
      901 ttcggtcgct gtgcgtgtac gatggcagtc cccagacggc ctactatgtg gccgccgcca
      961 cggccagctc ggaagtcccg gcggctcgtc ccgtggccct gccgctggcc agggacccgg
     1021 gccctacgga tctggcgcca ggtcccgtgg cactggagct caaagccgac cgcgaactca
     1081 cagccgcgac ccggtggatg gaggcgcgca tcgggctcga ggacggcatc cggctggtca
     1141 gtctgagatc gctcgaccca ccgctggagt ggctcgccgg gggtcagaag cccgagctgc
     1201 tccgggtact cgttgacggc cagcctgtgg cgtttgggcc atggacctgg gtgacggccg
     1261 aggaggcgcc cgtgctcgtg tcacactcgc gcacgggcgg accgccgctg tcggcgcgcg
     1321 tggaggtgcg agtggatcct ctccagggca tgggcctggt gccccagatc acgaacgagg
     1381 gtcccgaaga cctgaaggtc agcttcgagc tgatgtgtgt accgctcgcg gcgggtgacc
     1441 cttccgcgat ggcttactgc ttcgcgcagc gaggtacgct catcgacacg cggccgacgc
     1501 agctgcggat gctggcttca ggcctctacc cactgcagtt catcaccgcg ttcagccccg
     1561 gcgcgcgcat gggagcccac cttt
//
LOCUS       DAOSPL010001288         1582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_78545_length_1582_cov_3.664047, whole genome shotgun sequence.
ACCESSION   DAOSPL010001288 DAOSPL010000000
VERSION     DAOSPL010001288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78545_length_1582_cov_3.664047"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PLQ54_19050"
     CDS             <1..719
                     /locus_tag="PLQ54_19050"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOS95418.1"
                     /translation="AAQMDVRRSVADPVYDAQCNVLGSLNLLEAARATGVSKIIYAST
                     GGAVYGEPLELPVRETHPINPICPYGASKHTVEHYLFMYRANYGLEYTVLRYPNVFGP
                     RQDPHGEAGVVAIFSQLMLAGKQPRIFGRGDQTRDYVFVRDVVDANMLALEGGHGCIY
                     NVGTGVETNVNQVFAAVAQALGYSGQPDYAEERLGEISRICLDAGLLRSELGWTPRYT
                     FEEGIQEATAFYKDQAQSGI"
     gene            755..>1582
                     /locus_tag="PLQ54_19055"
     CDS             755..>1582
                     /locus_tag="PLQ54_19055"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOS95419.1"
                     /translation="MDVWVGRQPIFDGDVKVRAYEVLYRSGARGSRHVVGEDRATAET
                     IVHTFIDLGLETITGGRRAFLNFSREMLVGGFAELLPADAVVIEVLEHTGADDAVIEA
                     CDRLKRAGYRLALDDYVHGTGSHELLVPMADIIKVDFKRSSVEARLEAVRQLKRPGRV
                     MLAEKVESERQFEWARRNGYEHFQGFFFARPSNVHGEQIPQAKASYIRLLSVLREQTL
                     DLDELAGIIKSDVSLSHTLLRMLNSAGFAWRRRIESVRHALVALGEDATRRWLSLLCL
                     "
BASE COUNT          282 a          485 c          541 g          274 t
ORIGIN      
        1 tggccgcaca gatggatgtg cgccggtcgg tggccgaccc cgtctacgat gcccagtgca
       61 atgtgctcgg gagcctcaac ctgctggagg cggcgcgcgc caccggcgtg agcaagatca
      121 tctatgcctc gaccggcggt gccgtctacg gcgagccgct cgaactgccg gtgcgagaga
      181 cccatccgat caacccgatc tgcccctacg gtgcgtcgaa gcacacggtt gaacactacc
      241 tgttcatgta ccgcgccaac tatgggctcg agtacacggt gctgcgctac ccgaatgtgt
      301 tcgggccgcg gcaggaccca cacggcgaag ccggagtggt ggccatcttc agccagctca
      361 tgctggcagg taagcaaccc aggatcttcg gtcggggtga ccagacccgc gactacgtat
      421 tcgtgcggga tgtagtcgac gccaacatgc tggccctgga gggcggacac ggttgtatat
      481 acaacgtcgg aaccggcgtc gagacgaatg tgaaccaagt gtttgccgct gtggcgcagg
      541 cgctcgggta ctcgggccag cccgactacg cggaagagcg cctgggcgag atcagccgca
      601 tctgcctcga tgccgggctg ttgcgatctg aactgggctg gacaccgcgc tataccttcg
      661 aagaggggat ccaggaggcg accgccttct acaaggacca ggcacagtcg gggatctgag
      721 ctggctcgat ctgtgaacgg ttcggagtgg gagaatggac gtctgggttg ggcggcaacc
      781 gatcttcgat ggtgacgtca aggtacgggc ctatgaggtg ctgtaccgga gcggggctcg
      841 tggttcgcgc cacgttgtgg gcgaggatag ggcgacggcc gagacgatcg tccacacctt
      901 catcgacctg gggctcgaga ccatcaccgg aggccgccgg gctttcctca acttcagccg
      961 cgagatgctc gtcggcggct tcgcagagct gctcccggcc gacgccgtcg tcatcgaggt
     1021 gctggaacac accggggccg atgacgcggt catcgaggcc tgcgaccgac tcaagcgggc
     1081 cgggtaccgg ctcgcgctcg acgactacgt ccacggcacc gggtcgcacg agctattggt
     1141 gccgatggcc gacatcatca aggtggactt caagcggagc agcgtggaag cgcggctcga
     1201 ggccgtgcga caactcaagc ggcccggacg cgttatgctc gcggagaagg tggagtctga
     1261 gcggcagttc gagtgggccc gccggaacgg ctatgagcac tttcaggggt tcttctttgc
     1321 ccgcccgtcc aacgtgcacg gcgaacagat cccgcaggcc aaggcgagtt acatccgcct
     1381 gctcagcgtg ctgcgcgaac agacgctgga cctggacgag ctcgcgggca tcatcaagag
     1441 cgacgtgtct ctctcgcaca cgctgctgcg gatgctcaac tcggcgggct tcgcgtggcg
     1501 ccgccggatc gagtcagttc gccatgcact ggtggcgctt ggtgaggacg cgacgcgccg
     1561 atggctatcg ctgctgtgcc tt
//
LOCUS       DAOSPL010001289         1581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_78605_length_1581_cov_3.243775, whole genome shotgun sequence.
ACCESSION   DAOSPL010001289 DAOSPL010000000
VERSION     DAOSPL010001289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78605_length_1581_cov_3.243775"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PLQ54_19060"
     CDS             complement(<1..364)
                     /locus_tag="PLQ54_19060"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS95420.1"
                     /translation="MADERIRIGVVGGGFGASFQWHLDPGCVVEAVSDLREDRRNHLM
                     NTYQCAKAYNSLEELILDDAIDAVAVFTEAPNHVRHCVAVMNTGKHVISAVPAGCNLE
                     ELEELVEAKERNGVRYMMA"
     gene            complement(412..1068)
                     /locus_tag="PLQ54_19065"
     CDS             complement(412..1068)
                     /locus_tag="PLQ54_19065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95421.1"
                     /translation="MNGGSTLLTHLGFLVAGGAVVWSVEPSPPAERLGSVQGLLMLAL
                     AYLCGIGTYGLTFYYLQDWMSRRARGFFIHQDAELYQRVARKLRGQGLGWPDEEETDY
                     PTALPLEKQWVFDYCTLYVAQHGAKQLWTHLVYSWDILRLCVAAFAALAIGACFQIRR
                     SMTELASPGVYGPMMLLLAVLVLYMLWQRHRAYMREVWLAFEICAPEAGNDGVADATE
                     "
     gene            1216..>1581
                     /locus_tag="PLQ54_19070"
     CDS             1216..>1581
                     /locus_tag="PLQ54_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOS95422.1"
                     /translation="MAHSALAVHGGTPVTADPFPAWPQFSAEIIEAAMEPLRSGRCNY
                     WTGPLGMRFEQAFAQWCGARFGISTSNGTSALHVALAGMGVGPGDEVIVPSYTFIASS
                     MCVCQAGAVPVFADVEPESH"
BASE COUNT          282 a          555 c          472 g          272 t
ORIGIN      
        1 cggccatcat gtagcgcacg ccgttgcgct ccttggcctc gaccagctcc tcgagctctt
       61 cgaggttgca gccggccggg acggcggaga tgacgtgctt cccggtgttc atgaccgcca
      121 cgcagtgccg gacatggttg ggggcctccg tgaagacggc gacggcatcg atggcatcgt
      181 cgaggatgag ttcctcaagc gagttgtacg ctttggcgca ctggtacgtg ttcatcaggt
      241 ggttccggcg atcctcacgg aggtcgctga ccgcctcgac aacgcacccg ggatccaggt
      301 gccactggaa gctggcacca aagcccccac ccaccacgcc aatgcgaatc ctctcatccg
      361 ccaatgcgga tcgctcctct ctgaccggta tggactatgc cgtccctccg gctactcagt
      421 ggcgtccgcc actccgtcgt taccagcctc cggcgcgcag atctcaaacg ccaaccatac
      481 ctcccgcatg taggctcggt gccgctgcca gagcatgtac agcacgagta cggccagcaa
      541 cagcatcatg ggcccgtaga cgccgggact ggccagttcg gtcatggagc gccggatctg
      601 gaaacacgct ccgatggcga gcgcggcgaa agcagccacg cacaggcgca gaatgtccca
      661 cgagtacacg agatgagtcc agagctgctt ggcaccatgc tgggccacat acagagtgca
      721 gtagtcgaac acccactgct tctccagcgg gagagccgtc gggtagtcgg tctcttcctc
      781 atcgggccat cccaggcctt ggcctcggag cttccttgcc acacgctggt agagctcggc
      841 gtcctggtgg atgaagaacc cacgagcccg gcggctcatc cagtcctgca agtagtagaa
      901 cgtgagtccg taggtaccaa tcccacacag gtacgccagt gccagcatga gcaggccctg
      961 gaccgagccc agcctctcgg cgggggggct cggttcgacc gaccagacca cggctccgcc
     1021 cgccaccaga aaccccaggt gagtcaagag agtggacccg ccgttcatcg atgtcctccc
     1081 atcgtgccag ccgccgcgca acattgttcc ctgaccatgg gcctctggcc tccgcaaccg
     1141 atctgccacg gcagccaggc ctgcgacccg ccaccggcga acgggtaacc ccacacacct
     1201 gccgaggtga tcaccatggc tcacagcgca ctggcagtac acggcggcac tccggtcacc
     1261 gccgacccgt ttcccgcctg gcctcagttc agcgccgaga tcatcgaagc cgccatggag
     1321 cccctgcgct cgggcagatg caactactgg accggccccc tcggcatgcg tttcgagcag
     1381 gccttcgctc agtggtgcgg ggcccgattc gggatctcga ccagcaatgg tacctctgcc
     1441 ttgcatgtgg ctcttgcggg catgggcgtt gggcccggcg acgaggtgat cgtgccctcg
     1501 tacaccttca tcgcctcgtc catgtgcgtc tgccaggccg gcgccgtgcc ggtgttcgcc
     1561 gatgtcgagc ccgagtcgca c
//
LOCUS       DAOSPL010001290         1580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_78683_length_1580_cov_3.156066, whole genome shotgun sequence.
ACCESSION   DAOSPL010001290 DAOSPL010000000
VERSION     DAOSPL010001290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78683_length_1580_cov_3.156066"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PLQ54_19075"
     CDS             <1..582
                     /locus_tag="PLQ54_19075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95423.1"
                     /translation="ITGARPGGAGKSTVLASLLSFLPPGERIVCVAGRDLNSLPPHAC
                     LLAHEIGDGPYYGYIWGRDVGRWLGHARDGTRVASCIHADTIDQVYDVFASPALAIPR
                     DDWSLLRLAVFLHVDRQSGSIRRRVSTVYWTPPGRDWPQVVFRWDPDGDRFLQECDPG
                     LDGGEWDRARVDIVRVFHRGLRQLGEVRDALSP"
     gene            732..1334
                     /locus_tag="PLQ54_19080"
     CDS             732..1334
                     /locus_tag="PLQ54_19080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95424.1"
                     /translation="MRAPAVVPMAAIALVMLGVGHALAVVGNPVALTEQAEAYELKGQ
                     YSSAAAARDRAVSYAKRTLESLKSSNSAAAKRDRALAQSLIAENQRWAAEDRAGGGKL
                     VHVKEEMQFVAAKLQPVKALESRYQQLKAKGANKDAESARQQAVQQRKAFIATLNQKI
                     SALDGMVNRKEKGWEARKIEADYYRGVLARWQWSLATLSA"
BASE COUNT          253 a          510 c          527 g          290 t
ORIGIN      
        1 atcaccgggg ctcggccggg cggcgcaggc aagagcacgg tcctcgcctc cctgctctcc
       61 ttcctgccgc cgggcgagcg catcgtatgc gtcgccgggc gagacctgaa ctcgctgccc
      121 ccccacgcct gcctgctggc gcacgagatc ggcgatggcc cctactacgg ctatatctgg
      181 ggccgcgacg tgggtcgctg gctcggccat gctcgagacg gcacgcgagt cgcctcgtgc
      241 atccatgccg acaccattga ccaggtctac gacgtcttcg ccagcccggc gctggccatt
      301 ccgcgcgatg actggagtct gttgcgcctc gccgtcttct tgcatgtgga ccgccagtcg
      361 ggtagcatcc gtcggagagt ctctaccgtc tactggacac cccctggccg agactggccc
      421 caggtggtat tccgctggga tccggatgga gaccggttcc ttcaggagtg cgatcccggc
      481 ctggacggcg gtgagtggga ccgggcccga gtggatatcg ttcgggtgtt ccatcgaggc
      541 ctgaggcagc ttggcgaggt tcgcgacgca ttgtcgccat gagtggcgga accttcgcgg
      601 ccgacggcag ttgaggtaca cgggcacgcg tcgactccac cgcagagtgc tgttccagat
      661 ccgcagtcgg ggccagcccc cccaccttgg gtggtgcggc ggcccacata cacatgagga
      721 gttgtcaccg agtgagagcc ccagcagttg tccccatggc cgccattgcc ctcgtgatgc
      781 tcggcgtcgg gcatgcgttg gccgttgtcg gcaatcccgt ggcgctgacc gagcaggcgg
      841 aggcctacga gctcaaaggc cagtactcga gtgctgccgc cgctcgcgac cgggctgtga
      901 gctatgccaa gagaacgctc gaatcgctca agtccagcaa ctcggccgcc gcgaagcgcg
      961 atcgagctct ggcgcagagc ctcatcgccg agaaccagcg ttgggcagcc gaagaccggg
     1021 ctggcggcgg gaagctcgtt cacgtcaagg aagagatgca gttcgtcgcc gccaagctcc
     1081 agcccgtgaa ggctctggag agccgttacc agcagctcaa ggctaagggt gccaacaagg
     1141 acgcagagtc ggcgcgtcag caagccgtgc agcaaagaaa ggccttcatt gcgacgctaa
     1201 atcagaagat cagcgctctg gacggcatgg ttaaccggaa ggagaagggc tgggaggccc
     1261 gcaagataga ggctgactac taccggggcg tgcttgcccg ctggcagtgg tctcttgcga
     1321 cgctgtccgc ctaggtctgc cggcccactt gggtgccgtt accgtttggc gtttccgtgc
     1381 gtttcttgat tgggtcccgg tccggccggg gattgtgtta gctcggccgc ctcatctgtg
     1441 gaggagcgag tagcgttgca tagtgcctat tccgttgtac ttacccgccg gtccgcccac
     1501 cgcggactgg cgctcttggg cgcggcttgg ctcgccagcg tcgccactcc agcccatgca
     1561 gtggttccgg ggctcttcca
//
LOCUS       DAOSPL010001291         1580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_78687_length_1580_cov_2.897049, whole genome shotgun sequence.
ACCESSION   DAOSPL010001291 DAOSPL010000000
VERSION     DAOSPL010001291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78687_length_1580_cov_2.897049"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="PLQ54_19085"
     CDS             <1..1572
                     /locus_tag="PLQ54_19085"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS95425.1"
                     /translation="GGLMLAAGVVGLIGGMGCSVFTAIAAYGFGTDVRGALFRRIHEL
                     SFANLDRLETGRLITRLTSDVDQVQEAVAMLLRILVRAPLLVVGSLIMAVLTSPQLSW
                     LLAAVGPLVLLALVAISRKGQTLFLAVQERLDRLNVTLQENLAGVRVVKAFVRAEYEC
                     QRFERANVDLMEWGVRAACLMAIAMPIMMTLVSLGVAGVIWFGGWQVQAGTTKVGQLL
                     AFVSYLLQMLSSLMMVGMLIIRLAQAEASAQRIREVLDAEPEVRDAASVAESPERRGR
                     LEFERVEFSYDGQQADPVLRGVSFVAEPGQRVAIMGATGSGKSSLAHLVPRFYDVTGG
                     RVLVDGVDVRDMRQEDLRARIGVVLQDTVLFSGTIRDNIRYGRPDATDEEVEEAARQA
                     QAHDFIMAFPNGYETVLGQRGVNLSGGQKQRLAIARALLCHPSILILDDCTSAVDAAT
                     EAHIMRALGETSHPCTRLIVAQRIGSVVDADQILVLENGVVAAAGTHGELLERSSVYQ
                     EIARSQLGEVEVGRG"
BASE COUNT          241 a          468 c          562 g          309 t
ORIGIN      
        1 ggtggcctca tgctggcggc gggcgtggtg ggcctcattg gcggtatggg gtgctcggtg
       61 ttcacggcca tcgccgcgta tggcttcggg accgacgttc gcggggccct cttccggcgc
      121 atccatgagc tctcctttgc gaacctggac cgtctggaga ccggtcggct catcactcgg
      181 ctcacgagcg acgtcgacca ggtccaggaa gcggtggcca tgctcctccg catcctggtc
      241 cgagcgcccc tgctggtcgt gggtagcctc atcatggcgg tgctgacgag tccacagctc
      301 tcttggctgc tggccgctgt cggtcccctc gtgcttcttg ctctggtggc tatcagtcgc
      361 aagggtcaga cgctcttcct tgcggttcag gagagactgg atcggctcaa tgtcaccctc
      421 caggagaatc tggccggtgt gcgagtcgtc aaggcattcg tgcgggccga gtatgagtgc
      481 cagcgtttcg agcgggccaa cgtggatctc atggagtggg gtgtccgcgc cgcgtgcctg
      541 atggctatcg ccatgcccat catgatgaca ctggtgagcc tgggggtagc cggtgtcatc
      601 tggttcgggg ggtggcaggt ccaggcggga acgaccaaag tgggccagct cctggcattc
      661 gtgagctacc tgctgcagat gctctcttca ttgatgatgg tcggcatgct catcatccgc
      721 cttgcgcagg ccgaggcctc agcccagagg atccgcgagg tgttggacgc cgagcctgag
      781 gtgcgggacg cggcatcggt cgccgagagt cccgagcggc ggggccgtct cgagtttgag
      841 cgggtcgaat tcagctatga cggccagcag gccgacccgg tacttcgtgg cgtgtcgttc
      901 gttgcagaac cgggtcagag ggtggccatc atgggggcca cgggatcagg gaagtcgagt
      961 ctcgcccatc tcgtacctcg gttctacgac gtcacgggcg ggcgcgtcct cgtcgatggc
     1021 gtggacgtcc gggacatgcg acaggaggac ctccgagcga ggatcggtgt ggttctccag
     1081 gatacggtct tgttctcggg caccatccgc gacaacatcc gctacggcag gccggatgcc
     1141 accgacgagg aggtggagga ggccgcccgg caggcacaag cccacgactt catcatggct
     1201 tttcctaacg gctatgagac cgtgctcgga cagcggggcg tgaacctttc cgggggccag
     1261 aagcagcgat tggccatcgc tcgggccctg ttgtgccacc catcgatact gatactcgat
     1321 gactgcacga gcgcggtcga cgcggcgacc gaggcccaca tcatgcgcgc cctcggggag
     1381 acgtcgcacc cgtgcacccg gctgatcgtg gcccagagga ttggctcggt ggttgatgcg
     1441 gaccagattc tggtgctcga gaatggggtg gtcgcggcgg cggggacgca tggcgagctg
     1501 ctcgaacgga gctcggtcta ccaggagatc gcgcggtcgc agttgggcga ggtggaagtg
     1561 ggccgtggct agctcctctc
//
LOCUS       DAOSPL010001292         1579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_78784_length_1579_cov_2.564304, whole genome shotgun sequence.
ACCESSION   DAOSPL010001292 DAOSPL010000000
VERSION     DAOSPL010001292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78784_length_1579_cov_2.564304"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1382)
                     /locus_tag="PLQ54_19090"
     CDS             complement(165..1382)
                     /locus_tag="PLQ54_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95426.1"
                     /translation="MMTPCLWIPSVALSLVAATDALAARSYTFDGSMSREVLESYLSR
                     AVTAMDLLTPVGDVDDNIRMLTSIGAKYAGRVVYRWGGEADLPRLLGIARANAAKVHA
                     ADPQMILQAGVFEIVTTQVEELAIPAYVFDAFGVAPEDRHFDYEAMLYPSGRFRDHWS
                     PGSSVPDMRQLETRLWFYYAATQYIDIGCEGIHFGQVHLIGAEDEGFAAWWDMLSRVR
                     DYASAHARRRFVLCDAHTHGVHYQGDRLLFDFHAYPLRIREIPDRPQEGELAVGYIDS
                     IYGKSAGGIAPSGWSCEHLPFIVEFDNWGSSGKEGQAIGDWWTWGWDEIDWFAHQPEA
                     YRNQWLRYAWDWVRQTDPNGYVQMPASRCLHHPVDGRLSWYHANRPSAVMPDGFNQED
                     AIRTIWADNAVGE"
BASE COUNT          278 a          526 c          543 g          232 t
ORIGIN      
        1 gcagccgttc cgggaatcca accaggtcgc gaggcagctc gccggccgcg ggggcgcccc
       61 atatcccgac gagagccaga ccgatgagga cgacagaatg ctggacgcgc accgaggtca
      121 ctccacgagc gccgacagcg cttggctact gaagctcgga cgcgctactc acccactgcg
      181 ttgtcggccc aaatcgtgcg gatagcgtct tcctggttga acccatcggg catgaccgcg
      241 ctgggccggt tcgcatggta ccacgaaagc cggccatcga ccggatgatg gaggcagcgg
      301 ctcgcgggca tctgcacgta cccgttgggg tcagtctggc gcacccagtc ccaggcgtag
      361 cgcagccact ggttgcggta ggcctcgggc tggtgcgcga accagtcgat ctcgtcccag
      421 ccccaggtcc accagtcgcc gatggcctgg ccctccttgc cgctggatcc ccagttgtcg
      481 aactcgacga tgaagggcag atgctcgcac gaccacccgc tgggggcaat gcccccagcg
      541 ctcttgccgt agatcgagtc gatgtagccc acggcgagct cgccctcctg gggccgatca
      601 gggatctcgc ggatacgcag ggggtaggcg tggaagtcaa acagcaaccg gtcgccctgg
      661 tagtggacgc cgtgggtgtg ggcatcgcag aggacgaagc ggcggcgggc gtgtgcgctg
      721 gcatagtcgc ggactcgact cagcatgtcc caccaggcgg cgaaaccctc gtcttccgcg
      781 ccgatgaggt gcacctggcc aaaatggatg ccctcgcagc cgatgtcgat gtactgggtg
      841 gccgcgtagt agaaccacag gcgcgtctcg agctggcgca tgtcgggaac cgagctgccc
      901 gggctccagt ggtcgcggaa gcggccactg gggtacagca tcgcctcgta gtcaaagtgc
      961 cggtcctcgg gcgcgacacc gaaggcgtcg aagacgtacg cggggatggc gagctcctcg
     1021 acctgggtgg tgacgatctc gaacacgccg gcctggagga tcatctgcgg atcggcggcg
     1081 tgcaccttgg ccgcgttggc ccgagcgata ccgagcagcc ggggcaggtc ggcctcacct
     1141 ccccagcggt agacgacccg acccgcgtac ttggccccaa tggaggtcag catgcggatg
     1201 ttatcgtcga cgtcaccaac aggggtcaac aggtccatgg cggtgaccgc gcgggagagg
     1261 tagctctcga gcacctcacg gctcatggag ccgtcgaacg tgtaggagcg cgccgccagt
     1321 gcatcggtcg ccgcgaccag cgagagggcc acgctgggta tccacaggca tggggtcatc
     1381 attcgagggc ctcctggggc cgaggcgccc ggcagtggga tgggcaccca agacgatgcc
     1441 acactttggc tcgccggcac acccccaaac cactgacggc gggggcacga ctccgcgaca
     1501 cgctgttcgc ccgccgtcag cggcttccgc gccaagcgtc ggcccccacg ggcgaacccg
     1561 cgggggccga cggccaaca
//
LOCUS       DAOSPL010001293         1573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_79165_length_1573_cov_3.226614, whole genome shotgun sequence.
ACCESSION   DAOSPL010001293 DAOSPL010000000
VERSION     DAOSPL010001293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79165_length_1573_cov_3.226614"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..156
                     /locus_tag="PLQ54_19095"
     CDS             <1..156
                     /locus_tag="PLQ54_19095"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOS95427.1"
                     /translation="TEAAGATDVEFGETSVPMAEPVTVDRPFIFFIVDNGTGAVLFVG
                     RVLDPTA"
     gene            231..1541
                     /locus_tag="PLQ54_19100"
     CDS             231..1541
                     /locus_tag="PLQ54_19100"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOS95428.1"
                     /translation="MRRLLQLVLVVLVAANCSSGCKAPGPEGGERHFAAPLDIEAPGA
                     LVRSNKPHDPAPKVPDADLKALADGNREFACALYHELAGGEGNLVFSPMSISTAMAMA
                     YAGARGRTAEQLADVLHFDLPSERLHPAFNSICLALQPPQGADDKTVKLTMLNALWAQ
                     KGGHYLDSFLDTLAANYDAGMHELDIAGDPAGACKAINRWIKEATGGRIAELAPAGGM
                     NGVALAVTNAVYFRGYWNYQFDEKDTHDEPFHRLDGDRVTVKMMHQTHSFDYCEGDGY
                     QAVSLPYYFERQSMVVVLPQAGQFEHVEAALDGDFVSGLQGHLRGTRVELGLPRLGFS
                     GTAELAQVLARMGAPDAFSDGADFSGITGSPGLFISNVCHGAFLSVNEKWTEAAAATE
                     VAWKSMEEPSQPVRLIADRPFMFLIVDRPSGAILFMGRVMDPTA"
BASE COUNT          264 a          535 c          516 g          258 t
ORIGIN      
        1 accgaggccg ccggcgccac cgatgtagag ttcggcgaga cgtcggtgcc catggcggag
       61 ccggtcaccg tcgaccggcc gttcatcttc ttcatcgtgg acaacggcac cggggcggtc
      121 ctgttcgtgg gccgggtgct ggacccgaca gcgtaggcgc aggtagagaa ctaagccctc
      181 tcgaacaacc tggtgcgccg caccccgcac cgctcccggg agggtctact gtgcgccgac
      241 tgcttcagct cgtactcgtc gtcctcgtcg ccgccaactg ctcctccggc tgcaaggcgc
      301 cgggccccga gggcggcgag cggcacttcg cggcaccgct cgacatcgag gcgccgggcg
      361 cgctggtgcg gtcgaacaag ccccacgacc cggcgcctaa ggttccggac gccgatctga
      421 aggctctggc cgacggcaac cgcgagttcg cgtgcgcgct ctaccacgag ctggccggcg
      481 gcgagggcaa cctggtcttc tcgcccatga gcatctcgac ggccatggcc atggcctatg
      541 cgggggcgag aggccggacg gctgagcagc tcgccgatgt gctccacttc gacctgcctt
      601 cggaacggct gcaccccgca ttcaacagca tctgcctcgc gctccagcca ccccagggcg
      661 ccgacgacaa gacggtcaag ctcaccatgc tcaacgcgct ctgggcccag aagggcgggc
      721 actacctcga cagcttcctc gataccctcg ccgccaacta tgacgcgggt atgcacgagc
      781 tggacattgc cggcgatccc gcaggggcgt gcaaggccat caaccgctgg atcaaggaag
      841 ccacgggcgg gcggatcgca gagctcgcgc ccgccggcgg gatgaacggc gtcgctctgg
      901 cggtcaccaa tgcagtctac ttccgcggct actggaacta tcagttcgat gaaaaggaca
      961 cacatgatga gccctttcat cgcttggacg gcgaccgggt gacggtcaag atgatgcacc
     1021 agacgcactc gttcgactac tgcgagggcg acgggtacca ggccgtgagc ctgccctact
     1081 acttcgagcg ccagtcgatg gtggtggtgc tgccacaggc cggccagttt gaacatgtcg
     1141 aggcggcact cgatggggac ttcgtctccg gactgcaggg ccatctccgg ggaacacggg
     1201 tcgaactcgg cctcccgcgc ctcggcttct cgggcactgc cgaactggcc caggtcctgg
     1261 cccgcatggg cgcccccgat gcattttccg atggtgccga cttctcgggc atcacggggt
     1321 cgcccggcct gttcatctcc aacgtgtgcc atggggcctt cctctctgtg aacgagaagt
     1381 ggaccgaggc cgcggcggca acggaggtcg cgtggaagtc gatggaggag ccgagccaac
     1441 ccgtgcggct cattgccgac cggccgttca tgttcctcat cgtcgacagg cccagcggcg
     1501 ccatcctgtt catggggcgc gtgatggacc caacggcgtg agtgtcgaca accactgggt
     1561 aggtgatgca cgt
//
LOCUS       DAOSPL010001294         1572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_79244_length_1572_cov_2.788398, whole genome shotgun sequence.
ACCESSION   DAOSPL010001294 DAOSPL010000000
VERSION     DAOSPL010001294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79244_length_1572_cov_2.788398"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PLQ54_19105"
     CDS             complement(<1..540)
                     /locus_tag="PLQ54_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95429.1"
                     /translation="MILKWGYGVAAVPDGAAAELIRGVYRPDEVIEIGTDQGTKVGWH
                     RSRITSLGEDPLRCEMTVVCPMGADGMPHIHTGQRVLVFFCSEGSGYAFETHVLKLGR
                     EHGVLGSAIVSARLGPPEMLYHFQRREALRVVPRSRIEVTTTWPRVDGSGEPVTHVGI
                     MEDVSVSGVRVRIDHFDISA"
     gene            complement(671..>1572)
                     /locus_tag="PLQ54_19110"
     CDS             complement(671..>1572)
                     /locus_tag="PLQ54_19110"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOS95430.1"
                     /translation="RALPAGAGGQLTKNMVGMASDWVASAPELDPDETRRCLQALSAA
                     ESAFDLGVGAGGRLRWYPSSVVRPPHDPRRDPLGLACPLAPGGQPMVAVGYRVLVYRQ
                     MGTDGCVFAAQVTDVGPERAEAGTGAAGLVVVWVTPPRRFYHYQRREALRVVPRPQLV
                     ASVAYGGEEMPGSRLACVVEDLSVSGARLRTDEASAPLGRFLRQGHRVRVTLPLGVDE
                     DAEPPDLSGEIVRLERDRRDRGRLWMAVEWRSVAPSHARVLSRYVVETERERLRARSE
                     AREPRGWWQGRRAAGREPDGPLD"
BASE COUNT          261 a          623 c          465 g          223 t
ORIGIN      
        1 cgccgagatg tcgaagtggt cgatgcggac ccggaccccg ctgacgctga cgtcctccat
       61 gatgccgaca tgcgtgacgg gctcgcccga tccgtcgacg cgaggccagg tggtcgtgac
      121 ctcgatcctg gaccgcggca cgacgcgcag ggcctcccgg cgttggaagt ggtacagcat
      181 ctcgggtgga cccaggcgag cggacacgat ggccgagccc agcaccccgt gttccctgcc
      241 gagcttgagc acgtgcgtct caaatgcata gccactgcct tcgctgcaga agaacacgag
      301 cacgcgttgc ccggtgtgga tatgaggcat cccgtccgcg cccatggggc agacaaccgt
      361 catctcgcag cgaagcgggt cttcgcccag tgaagtgatc cgcgaccggt gccacccaac
      421 cttggtgccc tggtctgtgc cgatctcgat cacttcatcc ggccggtaca ccccgcggat
      481 cagctccgcc gccgcaccgt caggaaccgc cgcaaccccg taaccccact tgaggatcat
      541 cttggtggcc tatgacctcg cattccgcgg gtgcggcgcc agagggcgac cagacctctc
      601 gcgcacacac ccgtctcgcc cgcgctcgcg cacaccgaca cgagtcgcca ggcgagcccc
      661 tccgcggtcg tcagtccaac ggtccatcgg gctcgcggcc cgctgccctt cgtccttgcc
      721 accagccgcg cggctcccgc gcttcgctcc gggcacgcag acgctcgcgc tcggtctcca
      781 ctacatagcg ggacagcacc cgggcgtgcg acggggcgac gctccgccac tccacggcca
      841 tccacagcct gcccctgtcg cgccggtcac gctccagtcg tacgatctcc ccactcaggt
      901 ccgggggctc cgcgtcctcg tcgacgccca gcgggagcgt cacacgcacg cgatgcccct
      961 ggcgcaggaa gcgccccaac ggcgccgacg cctcatccgt gcgaaggcga gccccagaga
     1021 cgctcaagtc ctctaccaca cacgccagcc tcgatcccgg catctcctcg ccgccgtagg
     1081 ccacactcgc cacgagttgt gggcgaggaa ccacacgaag ggcctcccgg cgctggtagt
     1141 ggtagaaccg cctcggtggc gtaacccaga cgaccaccag acccgccgca ccggtacctg
     1201 cctcggcccg ctccggcccc acatccgtaa cctgggccgc aaacacacac ccatcggtgc
     1261 ccatctgccg atagaccagg acccggtacc ccacggccac catgggctgt ccgcccggtg
     1321 ccagcgggca tgccaggccc agaggatcac gcctcggatc gtgcgggggc ctcacgaccg
     1381 aggacgggta ccaccgcagg cgaccgccag caccgacacc caggtcgaaa gcgctttccg
     1441 cggcgctcag cgcctgcaag cacctccggg tctcgtccgg gtccagctcc ggcgcgctgg
     1501 ccacccaatc gctcgccata ccgaccatgt tcttcgtaag ttgaccgccg gctcctgccg
     1561 gtaacgcacg cc
//
LOCUS       DAOSPL010001295         1569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_79456_length_1569_cov_2.893659, whole genome shotgun sequence.
ACCESSION   DAOSPL010001295 DAOSPL010000000
VERSION     DAOSPL010001295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79456_length_1569_cov_2.893659"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /locus_tag="PLQ54_19115"
     CDS             complement(<1..1320)
                     /locus_tag="PLQ54_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOS95431.1"
                     /translation="MAVTLFRKVDYPLSKLVGDIEQGEIGLPDIQRPFVWPFAKARDL
                     FDSMYKGFPVGYLLFWENANGNGFRQIGVGGKQRKVPRLLIVDGQQRLTSLFAVLRGA
                     SVLTEDYQEARIRVAFRPRDHQFEVADAAIERDPEFIADISELWRGDKLRGEFVEEFI
                     ARLSASREVMDRERHLLGEAIDRVYDLQNYPFVVLELSADLDEEQVAEVFVRINAQGK
                     SLNQADFILTLLSVFWDEGRHALERFCRDARVPRADGRASSFNHFIRPAPDQMLRVGV
                     GLGFRRARLREVYSILRGKDLDTGEFSEERRDSQFAVLRRAQAQVLDLTHWHEFLKCL
                     VRAGFRSEAMVTSRTTVIYSYVLFLIGRLDMEADAFALRDAIARWFFMASLTGRYTGS
                     AESAMEEDLARLRTISSASSFLSLIDHIVGDSLTEDFWNITLPNNLET"
BASE COUNT          298 a          520 c          478 g          273 t
ORIGIN      
        1 tgtctccagg ttgttgggga gggtaatatt ccagaaatcc tccgtgaggc tatcacccac
       61 gatgtggtca atgagcgaaa ggaaactact ggcggagctg atggtgcgca agcgggccag
      121 atcctcctcc atcgcggact ccgccgaccc tgtgtaccgc cctgtgaggc tagccatgaa
      181 gaaccaccgg gcaatggcgt cgcgtagggc aaacgcatcc gcctccatgt cgagcctgcc
      241 gataaggaag agcacatagg agtagatcac ggttgtccgc gaggtcacca tagcttcgct
      301 ccggaacccc gcacgcacaa gacactttag gaactcatgc cagtgagtca ggtccaagac
      361 ctgagcctgt gcccgccgca gcaccgcaaa ctggctgtcc cttcgctcct cggagaactc
      421 gcccgtgtcg aggtctttgc cgcgcaggat cgagtacacc tcgcgcaagc gagcccgtcg
      481 gaaccccagc cccacgccta cgcgcagcat ctgatccggg gcgggcctga tgaagtggtt
      541 gaaggaagag gcgcggccgt cagcgcgcgg taccctcgcg tctcggcaga agcgctcgag
      601 ggcgtgtctt ccctcgtccc agaacacgga caggagcgtg aggatgaagt ctgcttggtt
      661 caacgacttc ccctgggcgt tgatccggac gaagacctcg gccacttgct cttcgtctag
      721 gtcggccgat agctcgagaa ccacgaatgg gtagttctgg agatcgtata cacggtcgat
      781 ggcctccccg agcaggtgcc tctctctatc catgacctcc cgactggcac tgagccgggc
      841 gatgaactcc tcgacgaact ccccgcgaag cttgtccccg cgccatagct cgctgatgtc
      901 ggcgatgaac tccgggtctc gctctatcgc cgcatcggcc acctcaaact ggtggtcacg
      961 gggccggaat gccacgcgga tgcgcgcctc ctggtagtcc tccgtcagga cgctggctcc
     1021 ccgcagcacg gcgaacagcg acgtgagtcg ctgctgaccg tcgacgatga gcagacgcgg
     1081 caccttgcgc tgcttgcccc ctacaccaat ctgtcggaag ccgttaccgt tcgcgttctc
     1141 ccagaacagg agatagccga cagggaaccc cttgtacatc gagtcgaaca ggtcgcgggc
     1201 cttcgcaaag ggccacacga acggacgctg gatgtcgggc aacccgatct cgccctgctc
     1261 gatgtctccc accaacttgc ttagaggata gtcgaccttc cggaagagcg tgacagccat
     1321 gggggatagc ctccgaacag gggtcggcgc gatcaggtga tcggtcaagg tcgagcacag
     1381 gagcggcatg gaccccgagc ggtgccgcgc ctattcagcg gttcgtccgt cgctcccttc
     1441 ctcacgtcgg cctgcggccg acccgggccg atggggggcc cacccccatc aggcccctcc
     1501 gaagggcaac gtcaagggcg gggcggggct gcccgcttgc cgccactccc tctccagggc
     1561 cgcgatgta
//
LOCUS       DAOSPL010001296         1567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_79588_length_1567_cov_3.113095, whole genome shotgun sequence.
ACCESSION   DAOSPL010001296 DAOSPL010000000
VERSION     DAOSPL010001296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79588_length_1567_cov_3.113095"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="PLQ54_19120"
     CDS             complement(<1..1109)
                     /locus_tag="PLQ54_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95432.1"
                     /translation="MRLALRFAVLVVALSAPARAVPQVEYVNPIAGFAIEIPEDWEMA
                     TGTGGNTEIAIDAPSGAAVVTQPALWFFRARHPAEEEARRLARDLQQINGGVAPTVAP
                     TGRPGEWEVSCRSSGVRGELVERWRCRDEAGQSYVVAALARPGVAEAFAAEIERAFDS
                     CRLIPTPIVRFFQEPTENAYRLVLPVGWTWEGEILRTGAIPGYFVWKVQSPDGLTGCF
                     SSPPAALNIMVPYTPADELAGGIVLQGLREQLPDAELDSVRRLPRVSDYFCELIKLAG
                     LGANPRVDKVRADYVGTRDGTRVRVRVDIGTFMLDASPLLDGRGDWFLMTGGSWAPEN
                     RFDERFPLARGVMASLGTSPKWRARQAEAVQSVLGR"
     gene            complement(1196..>1567)
                     /locus_tag="PLQ54_19125"
     CDS             complement(1196..>1567)
                     /locus_tag="PLQ54_19125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOS95433.1"
                     /translation="FSIATAVDDGIRNVPPVLVKAGRTLGARGVTLQLRVVLPAALPS
                     IVTGMKLGWSFAWRALMAGELLYSEKGLGCVLMMGRNLGDMAQVVAAMSVILILGLVV
                     TQYVFGPIERMMAQRWGLAHR"
BASE COUNT          262 a          554 c          534 g          217 t
ORIGIN      
        1 cggccgagca ccgattgcac ggcttcagcc tgccgcgctc tccatttggg cgaggtcccg
       61 agtgaggcca tcacgccgcg cgccagtggg aaccgctcgt cgaaccggtt ttcgggtgcc
      121 cacgagccgc cggtcatcag gaaccagtcc ccccggccgt cgagcagcgg tgacgcgtcg
      181 agcatgaatg tgccgatgtc gacccgcacg cggaccctcg tgccgtcgcg cgtgcccacg
      241 tagtcggccc ggaccttgtc gacccgaggg ttggcgccga gtccagcgag cttgatgagc
      301 tcgcagaagt agtcgctgac acgtggcagc cgacgcaccg agtcgagttc ggcatcgggc
      361 agctgctccc tgagcccctg gagcacgatg cctccggcca actcatccgc cggggtatag
      421 ggcaccatga tgttcagcgc cgcgggcggg ctcgagaagc atccggtcag gccatccggg
      481 ctctgcacct tccagacgaa gtagccgggt atggcaccgg tgcgcaggat ctcgccctcc
      541 catgtccacc ccaccggcag caccaggcgg tacgcattct cggtgggttc ctggaagaag
      601 cggacgatgg gcgtcgggat gaggcggcac gagtcgaagg cgcgctcgat ctcggccgcg
      661 aaggcttccg ccacgccggg gcgagccaat gcggcgacga cgtagctttg gccggcttcg
      721 tcgcgacaac gccagcgttc gacgagctcg ccgcgaacgc cgctcgatcg gcacgagacc
      781 tcccactcgc ccgggcgccc ggtgggcgcc acggtgggcg ccaccccgcc gttgatctgc
      841 tgcaggtcgc gggccagacg ccgcgcctcc tcctcagcgg ggtgccgcgc gcggaagaac
      901 cacagtgccg gctgagtgac caccgccgcc cccgacggcg cgtcgatggc gatctcggtg
      961 ttgccgccgg tacccgtggc catctcccag tcctcgggga tctcgatggc gaagccggcg
     1021 atggggttca catactccac ctgggggacg gcacgggcgg gagcggagag agcgaccaca
     1081 aggacagcga agcgaagggc gagccgcatg ggctattccc cctcggaagc ttcgagtgaa
     1141 aggccagagc cccagttcgc cacgcgcggg ctccagcctt cctcccgcgt ccccgctagc
     1201 ggtgcgccag cccccaccgc tgagccatca tgcgctcaat cgggccaaag acatactggg
     1261 tgaccaccag gccgaggatg aggatcacgc tcatcgcggc aaccacctgg gccatgtcgc
     1321 ccaggttccg gcccatcatg aggacgcacc cgaggccctt ctcggagtac agcagctcgc
     1381 cggccatcag ggcacgccag gcaaaggacc agccgagctt catccccgtg acgatgctgg
     1441 ggagcgcggc gggcagcacg acgcgaagct gcagcgtgac gccgcgggcg ccgagggtcc
     1501 ggcccgcctt gacaaggact ggcggcacat tgcggatgcc gtcgtccacc gcggtggcga
     1561 tggagaa
//
LOCUS       DAOSPL010001297         1566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_79659_length_1566_cov_2.673064, whole genome shotgun sequence.
ACCESSION   DAOSPL010001297 DAOSPL010000000
VERSION     DAOSPL010001297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79659_length_1566_cov_2.673064"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /locus_tag="PLQ54_19130"
     CDS             <1..1431
                     /locus_tag="PLQ54_19130"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOS95434.1"
                     /translation="WVALIVWRLGMGPRSALFVGFAVALSSTAVVLSLLERRAEVHTP
                     HGRVILGALLFQDVAVVPMMLAVPLLAGQRDEASPALLPLLAKGLAVLALFIIAGRWF
                     VPPLLYRVARSRERELFMLTVIFLALGVAWLTSQAGLSLALGAFLAGLIVSESEYSQR
                     ALGSIMPLRDVFTSFFFISVGMLLDVSVILQYPWLVLGLTVGVIGLKSALAGVGVALL
                     GLPLRTLILSGMALAQIGEFSFVLYSAGMAYGLLDEVQGAIFLAVAVGTLVLTPACIS
                     VAPWVASLAQRLPLPARWQGVHAPEYDGPPGALSDHLVIIGFGFNGAHLAQVAQEAGI
                     AYVVLEMNPDTVRRERQRGLPITLGDATQEATLRHLHIERARMVVVAISDAAASRRAT
                     ELARRLSPGCRLVVRTRFLGDVDTLKQLGADEVIPAEFETAVAIFRRVLECYCVPEER
                     ITSLVAQIRADNYHLLRSDQPLVPPR"
BASE COUNT          180 a          528 c          536 g          322 t
ORIGIN      
        1 tgggtggcgc ttatcgtctg gcgcctgggt atggggcccc ggtcggctct cttcgtgggg
       61 ttcgccgtgg cgctcagcag caccgcggtg gtcttgtcgc tcctcgagcg gcgagccgaa
      121 gttcacacgc cacacggccg cgtcatcctc ggcgcgctgc tgtttcagga tgtggccgtg
      181 gtgcccatga tgctggccgt gccgctcttg gccggccagc gcgatgaggc atcgccagcc
      241 cttctgcccc tgctggccaa gggattggcg gtgctggcgc tgttcatcat cgccggccgc
      301 tggttcgtgc ctccgctgct ctaccgggtc gcacgctcgc gcgagcgcga gctcttcatg
      361 ctcaccgtga tcttcctggc cctcggtgtc gcttggctga catcgcaggc cgggttgtcg
      421 ttggctcttg gcgcatttct ggccgggctc atcgtctcag agtcggagta cagtcagagg
      481 gcgctgggca gcatcatgcc gctgcgcgac gtcttcacca gcttcttttt catctccgtg
      541 ggcatgttgc tcgatgtgtc cgtgatcctc cagtacccgt ggcttgtgct ggggctgact
      601 gttggagtca tcgggctcaa gtcggccctg gcgggcgtcg gcgtggccct gcttgggttg
      661 cctctccgca ctctcatcct cagtggcatg gctctcgcgc agatcggcga gttctccttc
      721 gtgctctaca gtgccggcat ggcgtatggg ctcctcgacg aggtccaggg ggccatcttc
      781 ctcgcggtgg ctgtcggcac tctggtgttg actcccgcgt gcatatcggt agcaccctgg
      841 gtggcctcgc tggcccagcg gctgcccctg ccggcccgct ggcagggcgt gcatgccccc
      901 gagtacgatg gtccccccgg ggccctctcg gaccacttgg tcatcatcgg gttcggcttc
      961 aacggcgcgc atctggctca ggtcgcgcag gaggcgggta tcgcctacgt tgtcctcgag
     1021 atgaacccgg acaccgtgcg gcgtgaacgc cagcgcggcc ttcccatcac actgggggat
     1081 gccacccagg aggcgacgct gcggcacctc cacatcgaga gggctcgcat ggtggtcgtg
     1141 gccatctcgg acgccgcggc cagtcggcgc gctaccgagc tggcccgccg cctgagcccg
     1201 ggctgccgcc tcgttgtccg cacccggttc ctgggggatg tggacacatt gaagcagctc
     1261 ggggccgacg aggtgatccc cgccgagttc gagaccgccg tcgccatctt tcgccgcgtg
     1321 ctcgagtgct actgcgtgcc ggaggagcgc attacgagcc tggttgcgca gatccgggcc
     1381 gacaactacc acctgctgcg ctcggaccag cccttggtgc ctccgcggta gctgccccgg
     1441 gcccctgcga ggccattgtc ggcgactcag agctcgagca gcccctgggc ccggttgggg
     1501 aggggcgcca tgggcattgg cggtaactga gggctcccgt ggcccatcag cctggttgcg
     1561 gagggg
//
LOCUS       DAOSPL010001298         1565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_79726_length_1565_cov_2.894702, whole genome shotgun sequence.
ACCESSION   DAOSPL010001298 DAOSPL010000000
VERSION     DAOSPL010001298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79726_length_1565_cov_2.894702"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="PLQ54_19135"
     CDS             <1..1028
                     /locus_tag="PLQ54_19135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 32"
                     /protein_id="HOS95435.1"
                     /translation="PPRTGRMTLYVSSDGIHWGDPVTETEPTGDRSTLFYNPFRGVWV
                     YSIRSGGATGRTRQYAEHADLIAGAKWGADGPSWWVGADRLDPPRPDVGTRPELYNLD
                     CVAYESLMLGFFSIWYGQPPDRAKPNQVLLGFSRDGFNWTRPVRSAFLPVSERHGDWN
                     WANVQSAGGGCLVVGDELWFYVSGRAGVPGSTASGVCATGLAKLRRDGFCSMDAPPGS
                     EGYVVTRPVSFSGKYLFVNADASEGSLRAEVLDPGGRVLKGLSRDKCIALRSNSTRAR
                     IQWQGTPDLSSLAGQAVRLRFALRDAQLYSFWVSPDASGASHGYIAAGGPGFTGPIDT
                     VGAGDRQ"
     gene            complement(1062..>1565)
                     /locus_tag="PLQ54_19140"
     CDS             complement(1062..>1565)
                     /locus_tag="PLQ54_19140"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOS95436.1"
                     /translation="VAADGAVYFTEPGYGLEGRPSEQDAEGVYRLAPDGTVTRVVNDM
                     TKPNGIFVSPDGKTLYVADSEECIIRAYPLNADGSAGPGRTFADMKSEAEGVPDGMTL
                     DEKGHIYCTGGGGIWVFTPDGEHLGTIPVPEVPANCTFGGPGNTTLYITARHSVYRIE
                     LLVPGLW"
BASE COUNT          245 a          559 c          494 g          267 t
ORIGIN      
        1 atcccccccg gactggccgg atgaccctct atgtctcctc cgacggcatc cactggggcg
       61 atcccgtcac cgagaccgaa cccaccggcg atcgctcgac actcttctac aacccattcc
      121 gaggcgtatg ggtctacagc atccgcagtg gcggcgccac gggccgcacc cgccagtatg
      181 ccgagcacgc cgacctgatc gcaggggcca agtggggtgc cgatggcccc tcgtggtggg
      241 tgggcgccga ccggcttgat ccgcctcgac ccgacgtcgg cacccgccct gagctctaca
      301 acctcgactg tgtcgcctac gagagtctca tgctcggctt cttcagcatc tggtatggcc
      361 agccaccgga ccgggccaag cccaaccaag tgctcctcgg cttcagccgc gatgggttca
      421 actggactcg ccccgtacgt tccgccttcc tgccggtatc ggagcgccat ggcgactgga
      481 actgggccaa cgtccaatcc gcgggcggtg ggtgtctggt cgtcggcgac gagctatggt
      541 tctacgtcag tggacgtgcc ggcgtgcccg gcagcacggc gtcgggcgtc tgtgctaccg
      601 gcctggccaa gctccgtcgg gatggattct gctcgatgga cgcgccgccc gggagtgagg
      661 gctatgtcgt gacgcggccc gtgagcttct ccgggaagta ccttttcgtg aacgcggacg
      721 ccagcgaggg ctcccttcgg gccgaggtgc tggacccggg cggccgagtt ctcaaaggcc
      781 tctcgcggga taagtgcatc gcgctccgca gcaactccac ccgggcccgg attcagtggc
      841 aagggacacc agacctgtcc tctctggccg gccaagcggt ccgcttgcgg ttcgcactgc
      901 gtgacgccca gctatactcc ttctgggtga gcccagacgc ctccggggcc agccacggct
      961 acatcgccgc cggcggaccc gggttcaccg gccccatcga cacggtggga gccggagacc
     1021 gccaataggc ccgaccgtcc gcacactccc cgcaccaccg cctaccaaag gcccggcacc
     1081 agcagctcga tccggtagac cgagtgcctg gcggtgatgt acagggtcgt gttgccgggc
     1141 cctccgaagg tgcagttcgc ggggacctcc ggcaccggga tggtgcccag atgctcacca
     1201 tcgggggtga atacccagat gcctcccccg cccgtgcagt agatgtggcc cttctcatcc
     1261 agcgtcatgc catcgggcac accctcggcc tcgctcttca tgtcagcgaa ggtccgtccc
     1321 ggacctgccg acccgtccgc gttgagcggg tatgcgcgaa tgatgcactc ctcggagtcg
     1381 gcgacataga gcgtcttccc gtcagggctc acgaagatgc cattgggctt cgtcatatcg
     1441 ttcaccacac gggtaaccgt gccgtcgggg gcgagccgat agaccccctc ggcgtcctgc
     1501 tcggatggcc gaccctcaag tccgtagccg ggctcggtga agtagacggc accgtccgcg
     1561 gccac
//
LOCUS       DAOSPL010001299         1564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_79799_length_1564_cov_2.855533, whole genome shotgun sequence.
ACCESSION   DAOSPL010001299 DAOSPL010000000
VERSION     DAOSPL010001299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79799_length_1564_cov_2.855533"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PLQ54_19145"
     CDS             complement(<1..612)
                     /locus_tag="PLQ54_19145"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOS95437.1"
                     /translation="MRPPRIRRGPALLAAGFLILTGLVFGPCLCNRPAPVEAPSRGLL
                     PVGQLRAVWAHGTSTKTREATDRLIEKAVRGHFNCIYWLGFWWGGHAYFESTHVPMSP
                     SVEPGFDPLQYLCEQAHRRGIEVHVRFVNGDNGSAKPGPVFGAHPHWAQRLPSGTRRL
                     WYDFNKPEVRRFQAELMLEVAERYPIDGLQFDFALYSCVSPRMH"
     gene            complement(609..1118)
                     /locus_tag="PLQ54_19150"
     CDS             complement(609..1118)
                     /locus_tag="PLQ54_19150"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOS95438.1"
                     /translation="MGEHPEQPANRLPRYPGCCMCGTGERSGTSVTFYVVDDTVRVDF
                     TPTETHRGFPGIIHGGVLSALLDEAMGWAPTLKAGRFCVTGEMAIRFLRSVPIGRPIT
                     VIATADRIGRVCTASAEVVDAEGAVYARAEGKYVPLSIEECREVARALVAGPDTIPLA
                     EWLAEEARP"
     gene            1419..>1564
                     /locus_tag="PLQ54_19155"
     CDS             1419..>1564
                     /locus_tag="PLQ54_19155"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOS95439.1"
                     /translation="MLAAGAGQALPRQITLGAGIPPHVGGMVVNKVCGSSLKAAMLAA
                     GQIRA"
BASE COUNT          255 a          536 c          550 g          223 t
ORIGIN      
        1 gtgcatacgg ggtgaaacgc aagaatacaa tgcgaagtcg aactgaaggc catcgatcgg
       61 atagcgctca gctacctcga gcatcagctc ggcctggaac cggcgcacct cgggcttgtt
      121 gaagtcgtac cagaggcggc gggtgccgga gggcagtcgt tgcgcccagt gcgggtgtgc
      181 cccgaacacg ggcccgggct tggccgatcc gttgtcgcca ttgacgaatc gcacgtgtac
      241 ctcgatgccc cgccggtggg cctgctcgca gagatactgc agtgggtcga agccgggctc
      301 gaccgacggc gacataggca catgcgtgct ctcgaagtag gcgtggccgc cccaccagaa
      361 gccgagccag tagatgcagt tgaagtgccc acggacggcc ttctcgatca gccggtcggt
      421 ggcctcgcgg gtcttggtcg aggtcccatg ggcccagacg gcccggagct ggcccacagg
      481 gagcaggcca cggctgggcg cctcgacggg cgccgggcga ttgcagaggc acggaccgaa
      541 cacgaggccc gtcaggatca ggaaccccgc ggccagcagc gcgggaccgc gacggatgcg
      601 cggtggcctc acggccgggc ctcttcggcg agccactcag cgagtggtat cgtgtcgggt
      661 cccgcgacca gggcacgggc gacctctcgg cattcctcga tggagagggg cacgtacttg
      721 ccctcggcgc gggcatagac ggcgccctcg gcatcgacta cttcggccga ggcggtgcag
      781 actcgtccga tgcggtccgc cgtggcgata acggtgatgg gccggccgat cggcacggag
      841 cggaggaacc ggatggccat ctcgcccgtg acgcagaagc ggccggcctt cagcgtcggt
      901 gcccagccca tggcctcatc cagcagggca ctcagcacgc cgccatggat gatgcccggg
      961 aaccctcggt gggtctcggt gggcgtgaag tcgacgcgaa ccgtatcgtc caccacatag
     1021 aaggtgaccg acgtgccgct ccgctccccg gtgccgcaca tacagcagcc agggtagcgc
     1081 ggcaggcggt tcgcaggctg ctccggatgc tcacccatcg ccagttgccc ctcggggatc
     1141 atagacgcgc cccggctcgc ggagcaagac gcggcggagg cgatccctcg cacgggattc
     1201 tcgcggctcc gtgaaccgct gccgcatgtg acccgttgcc ctcttggcag ccagggccgg
     1261 gggcggtcgt ccgagcaaac gctcggagga tcgccctctc tatttgcctc ccccgcctgt
     1321 tgcaggcgct gccctccccc cagccgaacc actgtgcccg cgcgccaact gcgcgagacg
     1381 aggggggcga agcggtgcct cgaccgaccc aagccattgt gctcgccgcc ggggccgggc
     1441 aggctctgcc gcgccagatc acgctcggcg cgggcatccc gccgcacgtc ggcggcatgg
     1501 tcgtcaacaa ggtctgcggc agcagcctga aagccgcgat gctggcggcc gggcagatcc
     1561 gcgc
//
LOCUS       DAOSPL010001300         1560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_80080_length_1560_cov_3.476412, whole genome shotgun sequence.
ACCESSION   DAOSPL010001300 DAOSPL010000000
VERSION     DAOSPL010001300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80080_length_1560_cov_3.476412"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLQ54_19160"
     CDS             complement(<1..587)
                     /locus_tag="PLQ54_19160"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOS95440.1"
                     /translation="MRVLLTGAFGNIGAHAARSLIEQGHQVRLFDVRTPRAVRMAEAL
                     GSRADVVWGDIRDGEAVRTAVADREAVGHLAAIIPPAANERPAWARDITVGGTRNVIE
                     AARACKGPVRLVYCSSIALFGKTQHLPPPRTATDPIQITDEYTAHKAECEQMVRESGL
                     DWVILRMGACPPLSTLESSPSGRVNPMMFDVPLTDR"
     gene            complement(594..>1560)
                     /locus_tag="PLQ54_19165"
     CDS             complement(594..>1560)
                     /locus_tag="PLQ54_19165"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOS95441.1"
                     /translation="AMAWAIAGCGRHSRPDLIIVRTPAILESAGRVPTTTGPEDAAAD
                     TAPGMPTGVGGAPGLPMPNLAWGGSPTPPAPGSGMADAPPRAPGRPMVTVTPEESSRP
                     EVPDTQSMGVPELEALLEEDRVEVREAAEHELLARIDDAQAEVREAAARALGKAGKAV
                     LPAMVQRATAGDVAAKCDAALELGRLGALASKAVPKLIELLDDPAKAVGKAALEALPA
                     IGDQARPAQGTLVDMLKDPSPEVRAAAARGLAIAGESASRAIEALSGMKASGGLVYNI
                     AIGRAKKEFAEGQKGKVAGLKPLVSDPDPKVRRAVQEALAKIKEG"
BASE COUNT          207 a          566 c          531 g          256 t
ORIGIN      
        1 cggtccgtca gcggcacatc gaacatcatt gggttcaccc gaccagaggg actcgactcc
       61 agcgtgctca gaggcgggca cgcgcccatg cggaggatga cccagtcgag gccggactcg
      121 cgcaccatct gctcgcactc ggccttgtga gccgtgtact cgtccgtgat ctgtatgggg
      181 tctgtggctg tccgtggagg gggcagatgc tgcgtcttgc cgaacagcgc gatggatgag
      241 cagtagacga gacggaccgg acccttgcag gcccgagccg cctcgatcac gttccgagtg
      301 cctcccacag tgatgtcccg cgcccaggcg ggccgctcgt tggcggcggg tgggatgatg
      361 gccgcgaggt gacccacggc ttcacgatcg gcgacggcgg ttcggacggc ttcaccgtcg
      421 cggatgtcgc cccacaccac gtcggccctg gagcccaggg cctccgccat gcggacggcc
      481 cgaggagtgc ggacatcgaa gagccgcacc tggtgcccct gctcaatcag gctccgcgcg
      541 gcatgggcac cgatgttgcc gaaggccccc gtgagcagga ctctcatcgc ggcctagccc
      601 tccttgatct tcgccagtgc ctcttgcact gcccgccgga ccttggggtc ggggtcggag
      661 accaggggct tgaggccagc cactttgccc ttctggcctt cggcgaactc cttcttggcc
      721 cgcccgatcg cgatgttgta cacgagcccc ccggatgctt tcatgcccga aagggcctcg
      781 atggccctcg atgcgctctc gcccgcgatg gcgagacccc gagccgcggc ggcccgcacc
      841 tcgggtgatg ggtccttgag catatcgacc agtgtgccct gggctggccg cgcctggtcg
      901 ccgatggcgg gaagcgcctc cagcgcggcc ttccccaccg ccttggccgg gtcgtcgagc
      961 agttcgatca gtttcggcac ggccttcgac gccagcgcgc cgagccgccc cagctcgagc
     1021 gccgcgtcgc acttggccgc gacatcaccc gccgtcgcac gctggaccat cgccgggagc
     1081 accgctttcc ctgccttgcc cagggcccgc gcggctgctt cgcgaacctc tgcctgggca
     1141 tcatcgatcc gagcgagcag ctcgtgctcg gctgcctcgc ggacctccac gcggtcctcc
     1201 tcgagcaggg cttcgagctc gggtacgccc atcgattggg tatcaggcac ctcgggcctg
     1261 ctcgactcct ccggtgtcac ggtcaccatg ggccggcccg gggcgcgtgg cggggcgtcc
     1321 gccatgccgg aaccgggggc cggaggcgtg gggcttccgc cccatgcgag gttgggcatc
     1381 ggcagccccg gtgctccccc cacgcccgtg ggcatgccgg gcgcggtgtc cgctgcggca
     1441 tcctctggcc ccgtggtggt cggcacacgg ccggcgctct cgaggatcgc tggcgtgcgc
     1501 acgatgatga gatcggggcg cgaatgccgg ccacaaccgg caatggccca cgccatcgcg
//
LOCUS       DAOSPL010001301         1559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_80160_length_1559_cov_2.907580, whole genome shotgun sequence.
ACCESSION   DAOSPL010001301 DAOSPL010000000
VERSION     DAOSPL010001301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80160_length_1559_cov_2.907580"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..846)
                     /gene="lpxA"
                     /locus_tag="PLQ54_19170"
     CDS             complement(43..846)
                     /gene="lpxA"
                     /locus_tag="PLQ54_19170"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR01852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOS95442.1"
                     /translation="MSRIDPRAVVDPKACIAEDVEIGPFCVVEGDVVLDRGVRLLSHV
                     VVRAGTRIGEGTEVHPGSVLGGEPQSTGYRGERSQLVIGKHNIIREHCTLHRGLGEGA
                     VTQIGDHNMIMAYVHIAHDCRVGNHVVLTSHAGMAGHVTVEDHAFFGAMSGVHQFCRV
                     GKLAMLGGYTKVVQDVPPFMIVDGRDKRTARVVGLNVTGLRRAGVPSESRNALKAAYK
                     LLFRSALNTTQAIERVELDVPPSAEVEYLVGFLKQQRDGRLGRGLDHAR"
     gene            complement(843..1280)
                     /gene="fabZ"
                     /locus_tag="PLQ54_19175"
     CDS             complement(843..1280)
                     /gene="fabZ"
                     /locus_tag="PLQ54_19175"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011125568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOS95443.1"
                     /translation="MGSLPHRYPFLMVDRILEVEPGKRAVGLKNVTVNEPFFTGHFPG
                     QPIMPGVLILEAMAQVAIFMVKSLPGYAERLAMFTGIDNVRFRKPVVPGDQLLIETQM
                     VRQRGHVGKVIGYARVDGEVACEGEIMYALAPPGYPFEDGEAT"
     gene            complement(1372..>1559)
                     /locus_tag="PLQ54_19180"
     CDS             complement(1372..>1559)
                     /locus_tag="PLQ54_19180"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOS95444.1"
                     /translation="DDFGVPPRWPDEVGRHKALDLVGDLALLGARLHACVITMMGGHA
                     LNRALVREIQGTVSLVE"
BASE COUNT          278 a          567 c          450 g          264 t
ORIGIN      
        1 gagcccccgg acgccggccg gaggacgcgc gatccggtcg ggctatcggg cgtggtcgag
       61 cccgcgtccc agccgcccat ctctctgctg cttgaggaag cccaccagat actcgacttc
      121 ggccgaggga ggcacatcga gctccacgcg ctcgatcgcc tgcgtggtgt tcagtgccga
      181 ccggaagagt agcttgtagg cggccttgag ggcgttcctc gactccgaag ggacccccgc
      241 gcggcgtaag ccggtgacgt tcaggcccac cactcgggcc gtgcgcttgt cccggccgtc
      301 cacgatcatg aacggcggaa catcctgcac cactttcgtg tacccgccca gcatggccag
      361 cttacccact cggcagaact ggtgcacacc cgacatcgca ccgaagaagg catggtcctc
      421 caccgtcacg tggcccgcca tgcccgcatg gctggtcagc accacatggt tcccgacccg
      481 gcagtcgtga gcgatgtgca catacgccat gatcatgttg tggtcgccga tctgcgtgac
      541 agcgccctcg ccgagccccc ggtgcagcgt gcaatgctcg cggatgatat tgtgcttgcc
      601 gatgaccaac tgcgaccgct cgccgcgata gcccgtcgac tgcggctcgc cacccaggac
      661 cgaacccgga tggacctcag taccctcgcc gatccgcgtc ccagcgcgga ccacgacatg
      721 cgacaggagc cggacgcctc ggtccagcac cacgtcgccc tcgactacgc agaacgggcc
      781 gatctcgaca tcttcggcga tacatgcctt cgggtcgacg accgcccggg gatcgatccg
      841 gctcatgttg cctctccgtc ttcgaaggga taccccggcg gcgcgagagc gtacatgatc
      901 tcaccctcgc aagcgacttc gccgtctacc cgggcatagc caatcacttt tcccacgtga
      961 ccgcgctggc gaaccatctg agtctcaatc agcagctggt cgccgggcac caccggcttc
     1021 cgaaaccgca cgttatcgat ccctgtgaac atggcgaggc gctccgcgta gcccggcagg
     1081 ctcttcacca tgaagatcgc cacctgggcc atcgcctcca gtatcagcac accgggcatg
     1141 atcggctggc ccgggaaatg gcctgtgaag aatggctcat tcaccgtgac gttcttcagc
     1201 ccgacggcgc gtttccccgg ctccacctcc aggatccggt ccaccatcag gaacgggtac
     1261 cgatggggca agctccccat gatacccacg gtgttcagcg agaggactgc tgggggcccg
     1321 tcgccctgtc cctccatcgc ctctcccgcg gatgccgcag gttgggctga ctcactcaac
     1381 caggctcacc gtcccttgta tctcccgcac gagagcgcgg ttcagcgcat gtccacccat
     1441 catggtgatt acacacgcat gcaggcgagc ccccagtaaa gcgaggtcgc ccaccaggtc
     1501 gagggcctta tgccgcccga cctcatcggg ccatcgagga ggcacaccaa agtcgtcct
//
LOCUS       DAOSPL010001302         1558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_80243_length_1558_cov_2.554890, whole genome shotgun sequence.
ACCESSION   DAOSPL010001302 DAOSPL010000000
VERSION     DAOSPL010001302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80243_length_1558_cov_2.554890"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..952
                     /locus_tag="PLQ54_19185"
     CDS             59..952
                     /locus_tag="PLQ54_19185"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HOS95445.1"
                     /translation="MAPVELLTPTSWSEAALLLQESPETSAVLAGGTDLMSRTFMGKV
                     SPAPRRLVSLRAIPSMGTIWRADDGSLHLHARTTLAQIAADPAVRETYPALHEACTRI
                     GSPQIRARATLVGNVCSASPAADGAVAALCFGGTAEVVDDHSAFDVPLVSFFTGPRTT
                     CLKPGQIVRALRFPPPSPRSGSAFRRVGTRRAMDCAAVILAASVALDAAGRIESAEIA
                     LGSVAPTPIRVTEAETRLLGQAPLPAGFAEAARIASATCDPISDIRASAEYRRQVVRG
                     VLPLVLQQAAERAMAAERQGS"
     gene            953..1420
                     /locus_tag="PLQ54_19190"
                     /pseudo
     CDS             953..1420
                     /locus_tag="PLQ54_19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877849.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
BASE COUNT          209 a          536 c          554 g          259 t
ORIGIN      
        1 acgggcagag ggaaccgtgt gcctgcgcgt atgagggcag cccgaggggg agtgcgccat
       61 ggcaccggtg gagctcctga ctccaacgtc ctggtccgag gcggccttgt tgctgcagga
      121 gtcgcccgag acaagtgccg tcttggcggg cgggaccgac ttgatgtcgc ggaccttcat
      181 gggcaaggtg agtccggcac ctcgccgact ggtgtccctt cgcgccattc cctccatggg
      241 gacgatctgg cgtgccgacg atggctcgct tcacctgcat gcgcggacga cgctcgcgca
      301 gattgcggcg gacccggccg tccgcgagac ttaccctgcg cttcacgagg catgcactcg
      361 gattggttcg ccgcagattc gagcgcgggc cacgctggtt ggcaacgtgt gcagcgcctc
      421 gccggcggcc gatggcgcgg tggccgctct ctgcttcggc gggactgccg aggttgtcga
      481 tgaccacagt gcgttcgatg tgcccctcgt gtccttcttc accgggccgc gcaccacgtg
      541 cctcaagccg gggcagatcg tccgggctct tcggttcccg ccgccctcgc ctcgcagcgg
      601 cagcgccttc cggcgagtcg gcacccgccg cgccatggac tgcgcagcgg tcatcctcgc
      661 cgcatcggtg gctctcgatg ccgcagggcg gattgagagc gccgagatcg ccctcgggtc
      721 tgtggcgccc acccccatcc gggtcaccga ggcggagacg cgcctgctgg gccaggcgcc
      781 cctcccggcc ggcttcgccg aggcggctcg catcgcttcg gcgacgtgtg accccatctc
      841 ggatattcgc gcctcggccg agtaccgacg acaggtcgtg cgcggcgtgt tgcccctggt
      901 ccttcagcaa gccgccgagc gggccatggc ggcggagagg cagggaagct agatggccac
      961 cttggtgctg catctccgca tcaacggcgt cttccggcaa gtcccggtga caccgacgga
     1021 cacgctcctc tgcgtcttgc gcgaccggct gggcctcaca ggcaccaagc gagggtgcgg
     1081 tgagggcgag tgcggcgcgt gcacggtgct gttcaacggc gaggcggtct gcagttgcct
     1141 catccccgcc gccgaggccg aaggcgccga tgtcatcacg atcgagggtc tcgccgatgg
     1201 catccggctc catcccatcc agcaggcctt cgtggacgag gaggcgctgc agtgcggcta
     1261 ctgcacgccg ggcatggtgc tggcagccaa ggcgctgctc gatcgggtcc ccaacccgac
     1321 agaggacgag attcgcaccg cgctctgcgg caatctctgc cggtgtacgg gttacacgca
     1381 gatcgtccgc gccgtccagc gcgcggcagc cgtgttgcgg ggtgagacgt tgggcgccgg
     1441 cccgggcccg gttcgtgcgg ccgttggtga gcctgtggaa cgtatcgggg cccgcgagaa
     1501 ggtgaccggg cggaccgtgt tcgccggcga catgcagctc ccgggcatgc tccacgcg
//
LOCUS       DAOSPL010001303         1556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_80354_length_1556_cov_4.189873, whole genome shotgun sequence.
ACCESSION   DAOSPL010001303 DAOSPL010000000
VERSION     DAOSPL010001303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80354_length_1556_cov_4.189873"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..824)
                     /locus_tag="PLQ54_19195"
     CDS             complement(60..824)
                     /locus_tag="PLQ54_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOS95446.1"
                     /translation="MADRLEILRTLVDMSRRLGDPLAANVILGEGNTSARIDAETFFV
                     KASGTQLPTIGPDGFCEVRTEPLLALLDGPDLDDTQVKDALQAACVDPEGLRPSVETI
                     LHASLLSLPDVRFVGHTHPEAVNAILCARDVTEALTGRLFPDEIVVCGPASVYVPYVD
                     PGLPLARVVRDKVAEYISAWGMNPRVILMQNHGLIAIGASARDVENITAMASKAARIL
                     LGTHALGGPNFLSPSSLARISARPDEKYRQAMLSGR"
     gene            1030..1362
                     /gene="trxA"
                     /locus_tag="PLQ54_19200"
     CDS             1030..1362
                     /gene="trxA"
                     /locus_tag="PLQ54_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012798550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOS95447.1"
                     /translation="MANVPALTDQEYEAKVTHAPRPVIIDFGAEWCGPCKALAPILDE
                     VSQEFAGSVDAYQVDIAVSPEMAARFGVMSVPTLVVVKGGTEVKRSVGLVPKDRLRDL
                     FASVAATE"
BASE COUNT          254 a          524 c          505 g          273 t
ORIGIN      
        1 tgtaggcctc gaatgaagac tccatgtgca catgtccctt ctgactgcac cagggggagt
       61 tagcggccag acaacatggc ctgacggtac ttctcatcgg gccgcgccga gatccgcgcc
      121 agactgctgg ggctcaggaa gttgggcccg cccagggcgt gggtgccgag gaggatgcgg
      181 gccgccttac tggccatcgc ggtgatgttc tccacatcgc gggcgctggc gccgatggcg
      241 atcaggccgt ggttctgcat gagaatgacc ctcgggttca tgccccaggc gctgatgtac
      301 tcggccacct tgtcacgcac cacacgggcc agggggagcc ccggatcgac atagggcacg
      361 taaaccgatg ccgggccgca gacgacgatc tcatccggga acagccggcc ggttagggcc
      421 tccgtgacat cccgggcaca cagaatggcg ttcacggcct cggggtgagt gtggccgacg
      481 aagcggacat ccggcagcga gagcaggctg gcgtgcaaga tggtctccac cgagggccgg
      541 aggccctccg gatcgacgca tgccgcctgc aacgcatcct tcacttgggt gtcatccagg
      601 tcaggtccgt cgaggagcgc gaggagtggc tcggtacgca cttcacagaa gccgtcggga
      661 ccgatggtcg gcagctgggt gccgctggct ttgacaaaga acgtctcggc atcgatccgg
      721 gccgacgtgt tcccctcacc caaaatcaca ttggccgcca gcgggtcgcc cagccgacgg
      781 ctcatatcta ccagtgtgcg cagaatctcg agccggtctg ccacggtctc gtctcccacc
      841 tttacctgcg cgccgagccg ttactacccc gggccggccg gacccgctcg gaacgctccg
      901 gccgctctgt agggttctgg tataatgccc gggctctcgg actccggtct gcctgtccgc
      961 cctctgggcc gtcaggcggc tggactaccc cccggcccgg tgttcgccac acgccaagga
     1021 ggtcgaccca tggccaacgt gcccgcgctg accgaccagg aatacgaggc gaaagtcacc
     1081 cacgcgcccc gcccggtcat catcgacttc ggcgccgaat ggtgcggacc gtgcaaggcc
     1141 ctggcaccca ttctcgacga ggtgtcgcag gagttcgccg gttcagtgga tgcctaccag
     1201 gtggatatcg ccgtgagccc cgagatggcc gcgcggtttg gcgtcatgtc ggtaccgaca
     1261 ctggttgtag tcaagggcgg cacggaagtc aagcggtcgg tcggcctcgt ccccaaggat
     1321 cgcttgcgcg acctcttcgc atcggtcgcc gcaaccgagt agccatcacc agggggcatc
     1381 ccgccgtgta cgaatgggct tccggactcg ccgcgtcgct caacgacgtg ttcgcctcaa
     1441 gcctccagtc gggctctccc gttgcgtatg tgctggctct tctgggcggc atcctggcct
     1501 cgctgacgcc ctgcgtgctg cctatggccc ccgtcacggt gaccatcatc ggggga
//
LOCUS       DAOSPL010001304         1556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_80359_length_1556_cov_3.688208, whole genome shotgun sequence.
ACCESSION   DAOSPL010001304 DAOSPL010000000
VERSION     DAOSPL010001304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80359_length_1556_cov_3.688208"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PLQ54_19205"
     CDS             <1..1011
                     /locus_tag="PLQ54_19205"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOS95448.1"
                     /translation="KYYQAVTITDRMDYLSPMTNNLAYCLAVEKLMGIEVPERAQVAR
                     VILCEMSRIASHLVWLGTSAMELGAMSVFLYCFRERETLLEAWEDTTGGRITPSYICV
                     GGLLADLSPEFIPKVRRAIAPLPARFDDYERLLTENPIWLKRTRGIGRLTGDELINWG
                     VTGPMLRAAGVAWDLRKSQPYSLYDRFEFDIPTGQDGDVYDRYAVRVAEMRQSVRIIQ
                     QAIDALPGGAHMSEDRRVWMPPRERVRTDMHAMIHHFKIIEEGFSPPPGTAYQGIEGP
                     KGEIGFLVVSDGGPRPYRCRVRPPSLMNVQVLPAMVRGRLLADVIAILASADFVLGEV
                     DR"
     gene            1065..1529
                     /gene="nuoE"
                     /locus_tag="PLQ54_19210"
     CDS             1065..1529
                     /gene="nuoE"
                     /locus_tag="PLQ54_19210"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOS95449.1"
                     /translation="MVSQAGIDEIRRLAASFPRRESALLPALWVIQREEGYLPEESLR
                     VVARELSMEPSDVTSRASFYTMFALRPRGQYVIQVCQSISCALVGAQQVIDHLRGRLG
                     IEVGETTHDERFTLLTVECLASCGSGPVMQINEDYFEHLTVERVDEILDGLP"
BASE COUNT          255 a          500 c          532 g          269 t
ORIGIN      
        1 aagtactacc aagcggtcac cattactgac cgcatggatt acctgtcgcc catgacgaac
       61 aacctggcct actgcttggc cgtcgagaag ctcatgggca tcgaggtgcc cgagcgcgcc
      121 caggtcgccc gcgtgatcct gtgcgagatg agccgcatcg cgagccatct cgtgtggctg
      181 ggcacgagcg ccatggagct cggagccatg tccgttttcc tctactgctt ccgcgagcgc
      241 gagacgctgc ttgaggcgtg ggaggacacc accgggggcc gcattacgcc cagctacatc
      301 tgcgtcgggg ggctgctggc cgatctctcg cccgagttca tccccaaagt ccgccgggcc
      361 atcgcccctc tgcccgcacg cttcgacgac tacgaacggt tactgacgga aaatcccatc
      421 tggctgaagc gcacgcgcgg aatcgggcgg ttgaccggtg atgagctgat aaactggggt
      481 gtcaccggac ccatgctccg cgccgccggc gtcgcctggg atctccgcaa gtctcagcca
      541 tacagcctct acgaccggtt cgagttcgac atcccgacag gccaggatgg ggacgtatac
      601 gatcggtacg cggtccgggt ggcggagatg cgacagtcgg tacgtatcat ccagcaggcc
      661 atcgacgccc tcccgggcgg ggcgcacatg agtgaggacc ggcgggtgtg gatgccgcct
      721 cgggaacggg tacggaccga catgcatgcg atgattcacc acttcaagat catcgaggag
      781 ggcttctcac cgccaccggg caccgcctat cagggcatcg aggggcccaa gggtgagatc
      841 ggattcctcg tggtgagtga cggcggacct cggccctacc ggtgccgggt ccggccgccg
      901 tcgctgatga acgtccaggt cctcccggcc atggttcgag gacggctgct ggcggatgtg
      961 atcgcgatcc tggccagcgc cgacttcgtc ctgggcgagg tggaccgata gcgagtgcgg
     1021 ccccgcccct tggcggcggc atcacaccgg ggagggacag gcgcgtggtc tcccaggcgg
     1081 gcatcgacga gatcaggcgg ctcgcggcga gtttccctcg ccgggagtcc gcactactcc
     1141 cggcgctctg ggtcatccag cgcgaggagg gctatctgcc cgaggagtcg ctccgggtgg
     1201 tggcccggga gctgtcgatg gagccgtcgg acgtgaccag ccgggcgtcc ttctacacca
     1261 tgttcgccct gcggccgcgc gggcaatatg tgatccaggt gtgccagagt atctcgtgcg
     1321 ccctggtggg ggcccagcag gtcatcgatc atcttcgggg ccggctgggc atcgaggtgg
     1381 gcgagaccac gcacgacgag cgctttacgc tgttgacggt cgagtgcctg gcctcgtgcg
     1441 gctctggccc ggtgatgcag atcaacgagg actacttcga gcacctgacg gtcgagcgcg
     1501 tggacgagat tctggacgga ctgccctgat ggcccatccc gccgagaccc tctggg
//
LOCUS       DAOSPL010001305         1555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_80443_length_1555_cov_3.229333, whole genome shotgun sequence.
ACCESSION   DAOSPL010001305 DAOSPL010000000
VERSION     DAOSPL010001305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80443_length_1555_cov_3.229333"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="PLQ54_19215"
     CDS             <1..1233
                     /locus_tag="PLQ54_19215"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95450.1"
                     /translation="PGTSCFEAFPVTPRRRPPAYPEQGVGAGDATGTMLVMDVYQGLP
                     DVPRGTVKYIRIVEEVPKPVAAECTGPRLQYPVVSAMSHLAVKRLLGTVPVEEDGSAC
                     FRVPADVPVYFAALDENMMEVQRMRAFTQVRAGERTACIGCHEARITAPANAVPLAAR
                     REPSTIEPPPDGVHAPDFAYDVQPVLDRHCQRCHSGADPAGKLDLSDTRTNLFNVAYE
                     NLVTRGYVAYVDANSSATLPLRPAKYYGSHASKLVQTLLTTHRERVRLSKADFERLVT
                     WVDCNAPYYGTYVYGRKGTLGGRELLTGAVRAALQPVYDRRCASCHGGDVAAVTRVDF
                     RRVDRSPMLLAPLARDAGGTERCGQAVFPRDDDPDALAWAEGMRILASEVASNPREDM
                     LPERPPISDPLCRYVYRP"
BASE COUNT          236 a          517 c          557 g          245 t
ORIGIN      
        1 ccgggcacat cgtgcttcga ggcgtttccc gtcacacctc gccggcggcc gccggcctac
       61 cctgagcagg gcgtgggggc tggggacgcg acgggtacga tgctcgtcat ggacgtctac
      121 cagggcttgc cggatgtgcc gagaggcacg gtcaagtaca tccgcatcgt cgaggaagtg
      181 cccaagcccg tggcggcgga gtgcaccggg cctcgcctcc agtacccggt ggtcagcgcc
      241 atgagtcatc tggccgtgaa gcgccttctg ggcacggtgc ccgtcgagga ggacggctcg
      301 gcctgcttcc gtgtcccggc tgatgtgccg gtctacttcg cggcgctgga cgagaacatg
      361 atggaggttc agcggatgcg ggcgttcacg caggtgcggg cgggggagcg aaccgcgtgc
      421 atcgggtgtc acgaggcccg gatcactgcc ccggcgaacg ccgtgccgct ggccgctcgg
      481 cgggagccct cgactatcga gccgccgccg gatggcgtgc acgctccgga cttcgcctat
      541 gacgttcagc ccgtgctgga ccggcactgt cagcggtgcc attcgggtgc ggaccccgcg
      601 gggaagctgg atctctcgga cacgcggaca aacctgttca acgtggccta cgagaacctg
      661 gtgacccggg gctatgtggc ctacgtcgat gccaatagct ccgcgacact gcccctgcgt
      721 ccggcgaagt actacgggtc acatgccagc aagctggtgc agacgctgtt gacgacgcac
      781 cgcgagcgtg tgaggctgtc gaaggcagac ttcgagcgcc tggtgacctg ggtcgactgc
      841 aatgcgccct actatgggac ctacgtctat gggcggaagg gcaccctggg cggccgcgag
      901 ctactgaccg gcgccgtccg ggcggcgtta cagcccgtct atgaccggcg gtgcgcgtcg
      961 tgccacggtg gcgacgtggc ggccgtgaca cgagtggact ttcgccgagt ggaccgaagt
     1021 ccgatgctgc tggccccgct cgcgcgggac gccggcggaa ccgagcgctg tggccaggcg
     1081 gtgttcccga gggacgacga tcctgacgcg cttgcatggg ccgagggcat gcggatcctg
     1141 gcctcggagg tggccagcaa cccgcgggag gacatgttgc ccgagcgtcc gccgatcagc
     1201 gacccgctgt gccgatacgt gtaccggccg taagtgacaa cggacaccgc gagggtctgc
     1261 cccgcacccc gcttcgcggc cggacaccgc ggagctctgc cccgcacccc gccaggggac
     1321 ctgtcccctg gaccccatcc aggagcggcc tggaacatcc gccgcgaccg ccggcgaggg
     1381 accgcttggc tcgcccatca ttctgccaga tagacatcgc ggtaggcgag ggggcgcttg
     1441 gactcgccga tccccggcgt gatgccgatc cagtaaccgc ggccggtgcc gtcgttgtcg
     1501 ttcacgatga cgttgaggcc catgacgcga ccggggacgg gctgtatgcc catgg
//
LOCUS       DAOSPL010001306         1555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_80446_length_1555_cov_3.073333, whole genome shotgun sequence.
ACCESSION   DAOSPL010001306 DAOSPL010000000
VERSION     DAOSPL010001306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80446_length_1555_cov_3.073333"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..861)
                     /locus_tag="PLQ54_19220"
     CDS             complement(91..861)
                     /locus_tag="PLQ54_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOS95451.1"
                     /translation="MGEWNLAETTLGLSLDAGYRVAVLPVGATEAHGLHLPYSTDTLQ
                     VEAIARRACEAADARGARVVLLPTVPYGVNENTLGFPWTISLRPSTLFRVVTDVVSSL
                     AQHGVPKMLLLNGHGGNEFQPHLRELCRQTPVQIVLVNWYQAASTAQERLFAHGGEHA
                     DEMETSLVAALRPDLVRAELAGSGRTREPVLSALKEGWAWVARPWDRLTYDSGVGDPS
                     QAAAWKGDELLVGLTEKLAGLLVELSDAPVDELYPYRA"
     gene            1052..1222
                     /locus_tag="PLQ54_19225"
     CDS             1052..1222
                     /locus_tag="PLQ54_19225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95452.1"
                     /translation="MTATQQRETLERLIAELDAYVDRQPPLPQYRAGRVPTEVPLVNL
                     EELAEDNSQVAA"
     gene            1282..1413
                     /locus_tag="PLQ54_19230"
     CDS             1282..1413
                     /locus_tag="PLQ54_19230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95453.1"
                     /translation="MYPLANIGPIGLKELFFVVCLAAVLILGPAFLRWVLQNRVRED"
BASE COUNT          273 a          586 c          446 g          250 t
ORIGIN      
        1 gcccgatgaa atccaccggt gcgtcagccg tctcagggaa gaggccccct cggtcgaaat
       61 gctcaccgga gtcctcgccg cggcctgacg tcacgctcga tacgggtaca gctcatccac
      121 aggggcatcg gacagctcca ccagcaagcc ggcgagcttc tcggtcagcc cgacgagaag
      181 ctcgtcgccc ttccaggctg ccgcctggct cggatccccg accccgctat cgtaggtcag
      241 acggtcccac ggacgcgcaa cccacgccca cccttccttc agcgccgaca acaccggctc
      301 ccgcgtgcgg cccgacccgg ccagctcggc tcgcaccaag tccgggcgga gagccgccac
      361 cagcgaggtc tccatctcgt ccgcgtgctc tccgccgtgc gcgaacaggc gctcctgagc
      421 cgtactggcc gcttgatacc agttgaccag cacgatctgt accggcgtct gacggcacag
      481 ctcccgcaga tggggctgga actcgttccc gccatgcccg ttaagcaaca gcatcttcgg
      541 taccccatgc tgcgccagac tcgacaccac gtccgtgacg acccgaaaca gcgtgctcgg
      601 cctcaggctg atagtccacg gaaaacccag tgtgttctcg ttcaccccat acggaaccgt
      661 cggcagcagc accacccgcg caccccgtgc atctgccgct tcgcatgccc gtcgggcaat
      721 cgcctcgacc tgaagcgtgt ccgttgaata cggcagatgc agaccgtgcg cctcggtggc
      781 tcccacgggg agtacggcca cccggtagcc cgcatccagg ctcagaccta gagtggtctc
      841 ggccaggttc cactcgccca tctccgcctc ccaaacccaa gccggatgca gccacgcgca
      901 cccgcgaccg aagcgcaggt cacttcggat acccaagcca gggaacctcc ggggccactc
      961 gcgcgtatgt gggtttgaac agcggtcggt ctgtggaaca ctcagggtgt gtcagggggt
     1021 cgtgggtgct tggggtcggg agggctgccc gatgaccgca acacagcaac gcgagacact
     1081 cgaacgcctc atcgccgaac tcgacgcata cgtggatcgc caaccgcctc tgccccaata
     1141 ccgcgcgggt cgcgtcccca ccgaagtgcc actggtgaat ctcgaagaac tcgccgagga
     1201 caacagccaa gtcgccgcct gagccccgtc gcccgtgcgg tatgatgatg ccgaggctcg
     1261 actcgcctcg ggaggccaca cgtgtaccca ctcgccaata tcggtcccat tgggctcaag
     1321 gagctcttct ttgtcgtatg cctcgcggcc gtgctcatcc tcggccccgc gttccttcgc
     1381 tgggtcctcc agaaccgcgt ccgagaggac tagctgtggc gccccctact cggttcgcaa
     1441 gaccgtcgtc tcccatgccg caagtccgcc cacctcgtcc acccgctcgc tgccggcgag
     1501 ccggatgcgc accgtcgctc ggtcgatggg gtcgagcccc gcattcagaa gcacc
//
LOCUS       DAOSPL010001307         1555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_80460_length_1555_cov_2.642667, whole genome shotgun sequence.
ACCESSION   DAOSPL010001307 DAOSPL010000000
VERSION     DAOSPL010001307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80460_length_1555_cov_2.642667"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PLQ54_19235"
     CDS             complement(<1..396)
                     /locus_tag="PLQ54_19235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95454.1"
                     /translation="MRLGAAILVALAALGVGASAQEPGYAFIGQRITFNESARTPITS
                     TAFRGTFELTRVPTQAWLVLEVDDQFNAYVNGRLAGSIKTFTEGKARAYPVADLLRVG
                     RNVVAVEAYSALDKMSLGLDVRSDTLDGPA"
     gene            549..1541
                     /locus_tag="PLQ54_19240"
     CDS             549..1541
                     /locus_tag="PLQ54_19240"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS95455.1"
                     /translation="MRHLNWGIMATGMIADKFAHGLSTTDEATALAVGSRTLDRAKEF
                     AAKHGIARAYGSYDELIADPDVDAIYISLPNHMHAEWSIRCAEAGKHILCEKPVTVNA
                     PELERVLEVVRRCDVFFMEAFMYRCHPQWTRLREVLDAGTIGEVRIIESAFAFNMGMN
                     LDNIRMQNPAAGGGLMDVGCYCVSFSRLVAGQEPCALHAVGHIGAQSRVDEQATGALK
                     FPSGAVASFSCATQCGVPSKANVYGSQGWIQVTNPWFPADGNASLIVSAGDKQETIEV
                     THGRELYANEALTVAQYLDRRQAPAMSWEDSLGQMRALDALRASMGLVFDCERG"
BASE COUNT          286 a          500 c          526 g          243 t
ORIGIN      
        1 cgccggcccg tcgagggtat ccgaccggac gtccagcccg agactcatct tgtccagcgc
       61 gctgtaggcc tcgaccgcga caacgttcct gcccacgcgg agcagatcag cgaccgggta
      121 cgcgcgcgcc tttccctcgg tgaacgtctt gatgctcccg gcaagccggc cgttgacgta
      181 ggcgttgaac tggtcatcga cctcgagaac gagccacgcc tgagtgggca cgcgcgtgag
      241 ctcgaaggtg ccgcggaacg ccgtggaggt gatgggggtt cgcgcggatt cattgaaggt
      301 gatgcgctgg ccgatgaacg cgtaaccagg ctcttgcgcg ctggcgccga cgcccaaggc
      361 cgcgagtgcc accaggatgg cggcccctag cctcatgctc cggacctccg atgcagccaa
      421 gtcggctcct tggacacgcc ggcctggcgc gtgtctcgca gcaacccaca aggcctgcgg
      481 gccggggacc gagaacaaca acgcaggccg gaggacggca gtatcctcca agcaacgggg
      541 agggcggcat gagacacctc aactggggca tcatggccac aggcatgatc gccgacaagt
      601 tcgcgcacgg gctcagcacc accgatgagg ccacggcact agcggtcgga tcccgcacgc
      661 tggaccgagc caaggagttc gccgcgaagc acggcatcgc tcgagcctac gggtcctacg
      721 atgaactcat cgcggatcca gacgtggacg ccatctacat ctctctgccc aatcacatgc
      781 acgcggagtg gtccatccgc tgtgcggagg cgggcaagca catcctgtgc gagaagccgg
      841 taacggtcaa cgcgccagag ctggaacgcg tgctggaggt tgtccgcagg tgtgacgtgt
      901 tcttcatgga ggcgttcatg taccgctgcc acccgcagtg gacgaggctc cgtgaggtcc
      961 tcgacgcggg gacgatcggc gaggtgcgga tcatcgagag tgccttcgcc ttcaacatgg
     1021 ggatgaacct ggacaacatc cggatgcaga accctgcggc gggcggcggc ctgatggatg
     1081 tgggctgtta ctgcgtgtcg ttctcacgcc tcgtggccgg gcaggagccg tgcgcgctgc
     1141 acgcggtggg gcacatcggc gcgcagtctc gagtcgacga gcaggccacg ggcgctctga
     1201 agttcccttc gggagccgtg gcctcgttct cgtgcgccac ccaatgcggc gtgccgtcga
     1261 aggccaacgt ctacggcagc cagggctgga tccaagtgac gaacccgtgg ttcccggccg
     1321 acgggaatgc ctcgctcatc gtgtcggccg gcgacaagca ggagaccatc gaggtaaccc
     1381 acggccggga gctctacgcc aacgaagctc tcaccgtggc gcagtatctc gaccggcgcc
     1441 aagcgccagc catgagctgg gaagacagcc tgggccagat gcgagccctg gatgccctcc
     1501 gcgcctcgat ggggctcgtg tttgactgcg agagggggta atcgaccatt gaaca
//
LOCUS       DAOSPL010001308         1554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_80520_length_1554_cov_3.249500, whole genome shotgun sequence.
ACCESSION   DAOSPL010001308 DAOSPL010000000
VERSION     DAOSPL010001308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80520_length_1554_cov_3.249500"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PLQ54_19245"
     CDS             <1..673
                     /locus_tag="PLQ54_19245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950322.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOS95456.1"
                     /translation="IDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMAALQERITS
                     TVRGSITSVQAIYVPADDPTDPAPATTFSHLDATTMLSRAVFEQGLYPAVDPLEPTSR
                     ILDPRIVGEEHYRVAREVQATLQRYKDLQDIIAILGVDELSDEDKTVVERARRIQRFL
                     SQPFSVAQHFTGMEGRYVPLAETIGGFRAILDGEYDHLPIQAFYMIGTIDEAVQKAKT
                     LADAA"
     gene            746..1153
                     /locus_tag="PLQ54_19250"
     CDS             746..1153
                     /locus_tag="PLQ54_19250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOS95457.1"
                     /translation="MAKAFRVDILTKERCVLSGDIVSLVAPGASGYLGVRPGHAPLIT
                     ELKVGRLTLRPVSGQEVRIAISGGLLEVRAEATTVLAETAERAEEIDAERARRALERA
                     RGRLAGEMSGDTDVQRARLALLRALNRLDVAEH"
     gene            1214..>1554
                     /locus_tag="PLQ54_19255"
     CDS             1214..>1554
                     /locus_tag="PLQ54_19255"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOS95458.1"
                     /translation="MDKLFICGQRRLKGRVRCDGSKNAALAVLAAAVAVPGRCRIEKI
                     PPVSDILVMIEIIKELGASAEMTPDGALIIDSTSLRTCRAPEHLVRMMRGSFYMAGAL
                     LGRFRRAEVLLP"
BASE COUNT          256 a          514 c          528 g          256 t
ORIGIN      
        1 catcgacaac atctttcggt tcacccaggc cggatccgag gtctccgcgc tgctcgggcg
       61 tatgccgagc gcggtcggct accaaccaac cctcgccacc gaaatggcgg cccttcagga
      121 acgcatcacc tccacggtcc gcggctccat cacatcggtg caggccatct acgtccccgc
      181 ggacgacccc accgatccgg cgccggccac cacgttctcg cacctggatg ccacgaccat
      241 gctatcgcgc gccgtgttcg agcagggcct ctaccctgcc gtggacccgc tggagccgac
      301 gtcacgcatc ctggatcccc gcatcgtggg cgaagagcac taccgggtcg cgcgcgaggt
      361 ccaggccaca ctccagcgct acaaggacct ccaggacatc attgccatcc tgggagtgga
      421 tgagctctcc gacgaggata agaccgtcgt cgagcgggcc cgccggatcc agcgtttcct
      481 ctcacagccg ttctctgtgg cccagcactt cacgggcatg gaagggcgct atgtgccgct
      541 ggcggagacc atcgggggct tccgggcaat cctggacggg gagtacgacc atctgccgat
      601 ccaggcattc tacatgatcg gcaccattga cgaggccgtt cagaaagcca agacactggc
      661 cgacgcggcc taatgctgtg tgcgccgggg gcgtgtcgca ctgggcggcg cgcggcgcga
      721 gcgccccatc gcggaggctc gggcattggc aaaggccttc agagttgaca tcttgaccaa
      781 agaacgctgt gtgctgtccg gcgacatcgt ctcgctggtc gcgccgggag cgagtgggta
      841 cctcggggtt cgcccgggcc atgccccgct cattacagag ctcaaggtgg gccggctcac
      901 cttgcgtccc gtgtcgggcc aggaggtgcg catcgccatc agtggaggac tcctggaggt
      961 gcgggccgaa gcaacgaccg tgctggcaga gaccgcggaa cgggccgagg agatcgacgc
     1021 ggaacgggcc cgtcgcgctc tggagcgcgc gcgaggccgt ctggcgggag agatgtcggg
     1081 cgatacggat gtgcagcggg cccgcctggc cctccttcgg gctctgaacc ggctggacgt
     1141 ggccgagcac tagaccgcgc cgcgcggacg gggctaccag cggacgggtg agcatcccgg
     1201 gaggaactcg agtttggata agctcttcat atgcggccag cgccgcctca agggccgcgt
     1261 tcggtgtgac ggcagcaaga acgccgccct ggcggtgctt gctgctgctg tggctgtgcc
     1321 cgggcgttgc cggattgaga agatcccacc ggtgtccgac atcctggtga tgatcgagat
     1381 catcaaggag ctcggtgcgt cggccgagat gacaccggat ggcgcgctga tcatcgactc
     1441 gacctcgctg cgcacttgcc gggccccgga gcacctcgtg cgaatgatgc gcgggtcgtt
     1501 ctacatggct ggcgcgctgc tggggcggtt ccgcagggcg gaggtcttac tccc
//
LOCUS       DAOSPL010001309         1553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_80606_length_1553_cov_2.917891, whole genome shotgun sequence.
ACCESSION   DAOSPL010001309 DAOSPL010000000
VERSION     DAOSPL010001309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80606_length_1553_cov_2.917891"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1553
                     /locus_tag="PLQ54_19260"
     CDS             <1..>1553
                     /locus_tag="PLQ54_19260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95459.1"
                     /translation="PVQARRQIETLQADVRRDPLPYRYVWHDVPTHIPGLTAEGRPEA
                     YGAWTFDQGCLFASYGEPTGTVSTDVPVPAAGRYTMWVLSDATNLSYAAPWFGTASVA
                     GKEQFRYHHQGNIASVWMKMGEVAVDQPGPVRVDFTLDGAGAGGTYRRIYTLFLVDDP
                     AFVPQGTIRPPWTLAEYRDRAARAGAQPGDAYLSWVLDDAYTPLTQEVWADGTTPGRS
                     WPDAPVAGPDASRDLVLARDGVRAVQVGLRNLTADPMTLSVEPGPLEAAGRRYPSAVS
                     WRVVAFAPGGADRQSWTPFLLLRRPTITIPPLGVAGLWLTVDGRGVPPGDYVSMVRLT
                     GEGAPDHTLTLRVHVSPVAATPNQPVLVDGFTQPHEGEEYLRDYVDHGLKLWRGEMSK
                     TEMQRWGIRQLALHCRGADDLARIKALGLDYDDWFAVIMDEPSGDTEEKLKPYLDAAK
                     ALRELDPHVRISFNPGEAATLPTFQLLAPYCDFWLPYSLHLSPNWGGPEKWAIYKAKP
                     WMWYTTPCL"
BASE COUNT          235 a          561 c          525 g          232 t
ORIGIN      
        1 cccgtccagg cgcggcggca gatcgagaca ctgcaggcgg acgtgcggcg cgatccacta
       61 ccctatcgat acgtgtggca cgacgtcccg acccacattc ccggcctgac ggccgaaggc
      121 cggccagagg cttacggcgc gtggaccttc gaccaggggt gcctgttcgc cagctatggc
      181 gagccgacgg gcacggtcag taccgatgtg cccgtccccg ccgccggtcg ctacaccatg
      241 tgggtgctct cggacgccac caacctgagc tacgcggcgc cgtggttcgg cacggccagc
      301 gtcgcgggca aggagcagtt ccgctaccac caccagggca acatcgccag cgtgtggatg
      361 aagatgggcg aggtcgccgt cgatcagccg gggccggtac gggtggactt cacgctcgac
      421 ggcgccggtg cgggcgggac ctaccggcgc atctacaccc tcttcctggt cgacgatccg
      481 gccttcgtgc cccagggcac catccgccca ccgtggactc tcgccgagta ccgtgaccgg
      541 gcggcccgag cgggcgctca gccgggcgac gcatacttgt cctgggtact cgacgatgcc
      601 tacacgcccc tgacccagga ggtgtgggcc gacggaacga cgcccggccg ttcgtggccc
      661 gatgcccccg tggctggccc cgacgcctcc cgcgacctgg tgctagcccg cgacggcgtc
      721 cgtgctgtcc aggttgggct gcggaacctg acggcggacc cgatgacgct cagcgtagag
      781 ccagggccgc tcgaggccgc gggccgccgc tatcccagcg ccgtcagctg gcgcgtcgtc
      841 gcattcgcgc ctggcggcgc cgaccgacag agctggacgc ccttcctcct gctccgccgc
      901 cccaccatca ccatcccccc tctgggagtc gctggcctct ggctcaccgt cgatgggcgc
      961 ggcgtgccgc ccggcgatta cgtgagcatg gtcaggctca cgggcgaggg ggcgcccgac
     1021 cacaccctga cgctgagggt gcacgtgtcg ccggtggctg cgacgccgaa ccagccggtc
     1081 ctcgtggatg gcttcacgca gcctcatgag ggcgaggagt acctgcgcga ctatgtcgac
     1141 cacgggctga agctctggcg tggcgagatg agcaagaccg agatgcagcg atggggcatt
     1201 cgccagttgg ccctgcactg ccgtggcgcc gacgacctgg cgcgcatcaa agcgctgggg
     1261 ctggactacg acgactggtt cgccgtcatc atggacgagc ccagcggcga cacggaggag
     1321 aagctcaagc cctatctcga tgcggccaag gcgctgcgcg agctcgaccc gcacgtccgc
     1381 atctcgttca accccggcga ggcggcgacg ctgccgactt tccagctcct ggcgccctac
     1441 tgcgacttct ggctgcccta ctcgctgcac ctgtcgccca actggggcgg gccggagaag
     1501 tgggccatct acaaggccaa gccctggatg tggtacacga cgccctgcct gtg
//
LOCUS       DAOSPL010001310         1553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_80608_length_1553_cov_2.701602, whole genome shotgun sequence.
ACCESSION   DAOSPL010001310 DAOSPL010000000
VERSION     DAOSPL010001310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80608_length_1553_cov_2.701602"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="PLQ54_19265"
     CDS             <1..947
                     /locus_tag="PLQ54_19265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS95460.1"
                     /translation="RSEDIVTPALFCDLARRAAQRRERIRAAGVDAYRLVFGEADGLP
                     GLVVDAYAGHLVAELECPALRPYLGPVCEELCSSTDAHGVSLRTVDGLTTVCGAEPPE
                     LIEIHEPPHSFVVDPRGGQKTGWFCDQRENRQAVAEYADGVSVLDVFCYTGGFSVACL
                     AAGAVSAHLVDGSRRALELARRNLELAGHAGRAERTEGDAFDVLKRLGSAGKSYGLVV
                     LDPPKMLAPDGDRATAERAYRKLQAWAMRLVRPGGVLATFSCSGAIDRSRLDALVAEA
                     AGDRPCRILRRLAQPQDHPVLLSFRKTEYLKGLILELD"
     gene            complement(944..>1553)
                     /locus_tag="PLQ54_19270"
     CDS             complement(944..>1553)
                     /locus_tag="PLQ54_19270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95461.1"
                     /translation="GTHDQAGARSCGLTAAHLVAGGVVITEGVTAEASSEYPGHPASN
                     LLADDRGEPTGPFSLSALRALRHKLDSAPLPLELHAVLYTHELQLGQWLMPHIEVCDA
                     VSLWTWRAEELARLPESLDRCEAIIGDKRKLLGLYMWDYGNKAPMPLSLMERQCTLGL
                     QWLRSGRVQGLIFLASCICDLGLEAVEWSREWIRMHQAEVLG"
BASE COUNT          247 a          505 c          554 g          247 t
ORIGIN      
        1 ggcgctccga ggatatcgtc acacccgcgc tgttctgcga cctggcgcgg cgcgccgcac
       61 aacggcgcga gcggatccgc gctgccgggg tggacgcgta ccggctcgtc tttggcgagg
      121 ccgatggcct cccgggcctg gtggtggacg cgtacgcggg ccatctggtc gctgagctgg
      181 aatgcccggc gctgcggccc tacctcgggc ccgtctgtga ggagctgtgc tcgtccacgg
      241 atgcccacgg cgtgagcctt cggaccgtcg atggcctgac cacggtctgc ggcgccgaac
      301 cccccgagct catcgagata cacgagccgc cacactcgtt cgtcgtggac ccgcgaggcg
      361 ggcagaagac cgggtggttc tgcgaccagc gggagaaccg gcaggcagtt gccgagtatg
      421 cggacggcgt cagcgtgctg gacgtgttct gttacacggg aggtttcagc gtggcgtgtc
      481 tcgccgccgg cgcggtcagc gctcacttgg tggacgggtc gcgtcgggcc ctcgagctgg
      541 cccggcggaa cctggagttg gcggggcatg ccggccgggc ggagcggact gagggtgacg
      601 cattcgatgt gctgaagcgg ctggggagcg ccggcaagag ctacgggctt gtggtcctcg
      661 accccccgaa gatgctcgcg ccggacggcg atcgggcgac ggcggaacgg gcataccgga
      721 agctccaggc ctgggcaatg cgcctggtgc ggccgggagg cgtcctggcc acgttctcct
      781 gcagcggggc catagaccga tcgcgcctgg acgctctcgt ggccgaagcg gctggagacc
      841 ggccctgccg catcctcagg cggctcgccc agccgcagga ccatccggtc ctactctcgt
      901 tccgcaagac tgaatacctg aaggggctga ttctggagct cgactagccg aggacctcgg
      961 cctggtgcat tcggatccac tcgcggctcc actccacggc ctcgagccca agatcgcaga
     1021 tgcagctcgc gagaaagatc agtccctgga cgcggcccga ccgaagccat tggagaccca
     1081 gggtacactg gcgctccatg agcgacagcg gcatgggtgc cttgttgcca tagtcccaca
     1141 tgtacaagcc aagcagcttg cgcttgtctc cgatgatcgc ctcgcaacga tcgagactct
     1201 cagggagccg cgcgagctcc tccgcgcgcc aagtccagag agagacggca tcgcagacct
     1261 cgatatgtgg catgagccac tggcccagct gaagctcgtg ggtgtacagc accgcatgca
     1321 gttcgagggg tagtggggcc gagtcgagct tgtgacgcag cgcccgcaac gctgacagcg
     1381 agaaggggcc tgttggttca ccccgatcgt cggcgagcag attcgacgcc ggatggcccg
     1441 ggtactcaga ggatgcctcg gcggtgacgc cttcagtgat cactaccccg ccggcgacga
     1501 ggtgagctgc cgtgaggcca caactccggg cgccggcctg gtcgtgggtg ccg
//
LOCUS       DAOSPL010001311         1551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_80759_length_1551_cov_3.139706, whole genome shotgun sequence.
ACCESSION   DAOSPL010001311 DAOSPL010000000
VERSION     DAOSPL010001311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80759_length_1551_cov_3.139706"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="PLQ54_19275"
     CDS             complement(<1..1283)
                     /locus_tag="PLQ54_19275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase"
                     /protein_id="HOS95462.1"
                     /translation="MRIDPTPRHELSPYLYMQFMEPLGTTDSSVAASWDHGRNCWRED
                     LVECTRRLGPTLMRWGGCFASYYRWREAVGPREARVPMQNLLWGGFETNQVGTAEFVD
                     FCRQVGADALMTVNFESDFRPYWSHDATGRSRVGDAREAAEWVRYCNALDHAERRGHG
                     FPEPLHIPLWQIGNETSYDAGAIEPEAAARKTVEFARAMRAEDPTIRLIGWGDMGWTR
                     PMVDIAGEHVDYIAFHHVFSPDQGHDEPVLRTQEYRKDFGATWERLMGARDIHEARIR
                     GMCEQLQGTNMPLAMTECHFALTGRDRCDVMATWATGVAYARMANVHQRYGERLKIAT
                     MADFCGTRWQVNAIMVPTPGGQAFMMPVALVMSLYRHHTGERALDVTRVPGDLDAVAS
                     RTGDTVFLHVVNTNRTRSVKATLGVDGMRVRSGRV"
BASE COUNT          218 a          530 c          549 g          254 t
ORIGIN      
        1 tgcacccgac cggaacgcac acgcatgcca tccacaccca gtgtggcttt caccgatcgc
       61 gtgcggttcg tgttgaccac gtggaggaac accgtgtcgc ccgtccgact ggccacggcg
      121 tccaggtcac ccggcacgcg ggtcacgtcc agtgcccgct cgccggtgtg gtggcggtag
      181 agcgacatga ccagcgccac gggcatcatg aatgcctggc cgccgggagt ggggaccatg
      241 atggcgttga cctgccagcg ggtgccgcag aagtcggcca tggtggcgat cttgaggcgt
      301 tcgccgtagc gctggtggac gttcgccatg cgcgcgtagg cgacgccggt cgcccatgtg
      361 gccatcacat cgcagcggtc gcggcctgtc agggcgaagt ggcactcggt catcgccaga
      421 ggcatgttcg ttccctggag ctgttcgcac atgccgcgga tccgggcctc gtggatgtcg
      481 cgcgcgccca tgagtcgctc ccatgtcgcc ccgaagtcct tgcggtactc ctgggtgcgc
      541 aggaccggct catcgtgacc ctggtcgggg ctgaacacat ggtggaaggc gatgtagtcg
      601 acgtgctcgc cggcgatgtc gaccatgggc cgcgtccagc ccatgtcgcc ccaaccgatg
      661 aggcggatgg tcgggtcctc ggcgcgcatg gcccgggcga actccacggt cttgcgcgcg
      721 gcggcctcgg gctcgatggc cccggcgtcg taagaggtct cgttgccgat ctgccagagc
      781 gggatgtgca gcggctctgg gaagccgtgg ccgcgccgtt cggcgtgatc cagcgcgttg
      841 cagtagcgca cccactcggc cgcctcgcgg gcgtcaccga cccggctgcg gcctgtcgcg
      901 tcgtggctcc agtaggggcg gaagtcggac tcgaagttca cggtcatgag cgcgtcggcg
      961 cccacctggc ggcagaagtc gacgaactcg gccgttccca cctgattggt ctcgaagccg
     1021 ccccagagca ggttctgcat cggcacgcgg gcctcgcgcg gcccgacggc ctcgcgccag
     1081 cgatagtatg aggcgaagca accgccccaa cgcatgagcg tgggcccgag gcgccgcgtg
     1141 cactcgacca ggtcctcgcg ccagcagttg cggccatggt cccacgaggc ggccaccgag
     1201 ctgtcggtgg ttccgagcgg ctccatgaac tgcatgtaga ggtacggcga cagctcgtgt
     1261 ctgggtgtcg gatcgattct caaggtccgc tctcctcccg acgcacgcgc cggcagcgcg
     1321 gctgccgcta tggctccgat gctggccgag cgtatgaact ccctacgtgt ggctctcatg
     1381 gcgctccctt cgcgacgccg attcgcgcct gcggctccgc gggcctccgg cgtgcgcgca
     1441 ggctggttct ggagcgcgga gcgcccgctc cgcagtagcg gggcacggca acggcacggg
     1501 gcaggaggcc tgcgggcacg aagcgcatgg ccctcgcgcc accgcgtgtc g
//
LOCUS       DAOSPL010001312         1549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_80913_length_1549_cov_2.965863, whole genome shotgun sequence.
ACCESSION   DAOSPL010001312 DAOSPL010000000
VERSION     DAOSPL010001312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80913_length_1549_cov_2.965863"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1150
                     /locus_tag="PLQ54_19280"
     CDS             155..1150
                     /locus_tag="PLQ54_19280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95463.1"
                     /translation="MLLRTVSVLACLALVGGAVWSEPGRPDRDPPPNNGVQSRGEGTP
                     SGTDGADSDPSASAERVRRRETIRYQPPVGSEARYVCTMNGRATIRFMGDKPLVGGAD
                     MTQKVERLDPDGMVYVSTFIQNGHAALGDKKEYHTEDRLLLAQFDALHQMHKVERVPT
                     DEEKAAQAAGETEDDGEEAIDLATILLYLLTSVDYHQRSMGVGTKWQTTYSVIDANNK
                     QITMGGTNELVGFVFDGTRRVAVIHSLFDIPVRGRSGTLDLQGSLACDVISEVYVDTG
                     EIRYRRAISNGEMKPSGSLLPIKIDISNLETAMGMVLPDRRPATALWSPEATGTE"
     gene            complement(1265..>1549)
                     /locus_tag="PLQ54_19285"
     CDS             complement(1265..>1549)
                     /locus_tag="PLQ54_19285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95464.1"
                     /translation="EIHWGQGHPASGGAPFFGAYGQASYRTGKETVFARYEYFDPNRT
                     AANDLWRGPLIGISHQFSARNKITAEVDFFENEATAGSDTRMGIRWQTKW"
BASE COUNT          276 a          501 c          533 g          239 t
ORIGIN      
        1 gggccacggg agccctcagt taccgccaat gtccgttgga ccaaggtccc ttgcgttacg
       61 gcctcaactg gaagagaatc cgtgcgccct ggcaaagtcg cggctccgga gactggtgtg
      121 cggtgccggt cggcccgggg gcttggggga tgccatgttg ttacgcaccg tgtcggttct
      181 ggcgtgcctt gccctggtgg gcggcgccgt gtggagtgag cccggccggc cggaccggga
      241 cccgccgccc aacaacgggg tccagtcacg cggggaaggc accccatcgg gcaccgatgg
      301 ggcggactcg gacccgtcgg ccagcgcgga acgggtccgg cgccgggaga ccattcgcta
      361 ccaacccccg gtgggcagcg aggcgcgtta tgtctgcacg atgaatggcc gggccaccat
      421 ccgcttcatg ggggataagc ccctggtggg cggggcggac atgacccaga aggtcgagcg
      481 gttggatccc gacggcatgg tgtacgtgtc gacgttcatc cagaatggcc acgccgcgct
      541 gggcgacaag aaggagtacc acaccgagga ccgcctgctc ctggctcagt tcgacgccct
      601 tcaccagatg cacaaagtcg agcgggtccc taccgacgag gagaaggcgg cgcaggccgc
      661 cggggaaacc gaggatgacg gggaagaggc catcgatctg gccaccatcc tgctctacct
      721 actgacgtcg gtggactacc accagcgcag catgggtgtg ggcaccaagt ggcagaccac
      781 atactcggtc atcgacgcca acaacaagca gatcaccatg ggcgggacca acgagctggt
      841 cggcttcgtc ttcgatggca cgcgccgcgt ggcggtgatc cattcgctgt tcgacatccc
      901 ggtacggggc cgatcgggca cgctggatct tcagggcagt ctcgcctgcg atgtcatcag
      961 cgaggtgtac gtcgacaccg gcgagatccg gtaccgccga gcgatctcca atggcgagat
     1021 gaagccctcc ggctcactgc tgcccatcaa gatcgacatc agcaacctcg agaccgcgat
     1081 gggcatggtc ctgccggacc ggcgcccggc gacggcgctg tggtcgccgg aagccaccgg
     1141 cacggagtag cccgccggtc gcaggcgccg cagaggcaac ccgctcgagc cggacaagag
     1201 acaagaaccg ggcagggccg cgcgcggccc tgcccgttga catgcgacct tcaggcgccc
     1261 cggcctacca tttggtctgc cagcggattc ccatgcgggt gtcggacccg gccgtggcct
     1321 cgttctcgaa gaagtccacc tcggccgtga tcttgttccg agcgctgaac tggtgggaga
     1381 tgccgatcag tggaccacgc cagaggtcgt tggccgcggt ccggttgggg tcgaagtact
     1441 catagcgagc gaacacggtc tccttaccag tccggtatga ggcctggccg taggccccaa
     1501 agaagggggc tcctccggaa gccggatggc cctggcccca gtggatctc
//
LOCUS       DAOSPL010001313         1549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_80917_length_1549_cov_2.791834, whole genome shotgun sequence.
ACCESSION   DAOSPL010001313 DAOSPL010000000
VERSION     DAOSPL010001313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80917_length_1549_cov_2.791834"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PLQ54_19290"
     CDS             complement(<1..294)
                     /locus_tag="PLQ54_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOS95465.1"
                     /translation="MVRETLVFILAGGKGNRLMPLTRYRSKPAVPFGSQYRIIDFTVS
                     NCINSGLRRIYVLTQYKSNSLAWHVRYAYGHHFVPEAGHFLTTVPPQQLTSEDW"
     gene            487..1167
                     /locus_tag="PLQ54_19295"
     CDS             487..1167
                     /locus_tag="PLQ54_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015172375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOS95466.1"
                     /translation="MEYQIRGTTMQTLDIQLPMGESIFTESGAMGWMSDNIHMETGMR
                     GGLMGGISRSLAGESMFLVDYTCMGGTGLISFCAGAPGKIMPLQLAPGQSMICQKDSF
                     LCAEKSVKLEMHFRRKLGAGLFGGEGFILQKLTGPGLAFVELDGEITEYTLEAGQGLR
                     IDTGHLAMYEPSIDFDIVTMKGIKNILFGGEGLFLAHVKGPGRVWLQSMPISNLAGRL
                     ARYLPSKG"
     gene            1183..>1549
                     /locus_tag="PLQ54_19300"
     CDS             1183..>1549
                     /locus_tag="PLQ54_19300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95467.1"
                     /translation="MKDQDGLRILWVVFGITAAAVFGAIVLAGVLLGWVTVGLSLLGL
                     VCAGVVFSVVLAWPLAHGVIWIYYAVRPDDRAPRADYSLDQSREAGEDHRRGSSSWTC
                     GRRRDSERARSSASSRLWIW"
BASE COUNT          274 a          446 c          543 g          286 t
ORIGIN      
        1 ccagtcctcg gatgtgagct gctggggggg caccgtggta aggaagtgcc cggcctcggg
       61 gacgaagtga tgcccgtagg catatctgac gtgccacgcc agggagttgg acttgtactg
      121 agttaaaaca tatatccggc gcaaaccaga gttaatgcag ttgctcaccg taaaatcaat
      181 gattcggtac tgcgagccga acggcacagc cggcttcgag cggtaccgag tgagcggcat
      241 gagccgattg cccttgcccc ccgccaggat gaacaccaga gtttcccgga ccacgggctc
      301 ccgccctccc ctcgccaccc gacctttcct cagtttccat tgtgccacct cctgggcccg
      361 gctccaaccc gccagcgtct ggcggggcgg cagggagggg accgagtgac ggggacagga
      421 actcggggcc caacaccgaa gcaacaggtg cagcgccagt cgaggtgcga gcggaggtgt
      481 gcagcagtgg aataccagat ccgcggcacg accatgcaga cactggatat ccagttgccc
      541 atgggcgaat cgatcttcac cgaatcgggg gccatgggct ggatgtccga caacatccac
      601 atggagaccg gaatgagggg ggggctgatg ggaggtatca gccgctcgct ggcgggcgag
      661 tccatgttcc tggtggacta cacctgcatg ggtggtaccg gcctgatcag cttctgcgcc
      721 ggtgctccgg gcaagatcat gccgctgcag ctggcgcccg gccagagcat gatctgccag
      781 aaagactcgt tcctgtgcgc cgagaagtcg gtgaagctgg agatgcactt ccggaggaag
      841 cttggcgccg gcttgtttgg tggcgagggc ttcattcttc agaagctaac gggtccgggg
      901 ctggcctttg tcgagctcga cggcgagatc accgagtaca ccctcgaagc gggccagggc
      961 ctccggatcg acacagggca cctggccatg tacgagccca gcatcgactt cgacatcgtc
     1021 accatgaagg ggatcaagaa catcctgttt ggtggcgagg gtctgttcct cgctcatgtg
     1081 aagggtccgg ggagggtctg gctgcagagt atgcccatct cgaacctggc ggggcggctg
     1141 gcgcgctacc tgccctcgaa aggctgaggg cgaggtcagg cgatgaagga tcaggacggg
     1201 ctgcggatcc tctgggtcgt gtttggcatc accgccgcgg ccgtgttcgg cgccatcgtg
     1261 ttggccggtg tgcttctggg ctgggtgacg gtcgggcttt cgctgctggg cctggtatgc
     1321 gctggggtgg tgttctcggt ggtcctggcg tggcctctgg cgcatggggt gatatggatc
     1381 tactatgcgg tccgaccgga tgaccgggct ccgcgggcgg actactccct cgaccagagc
     1441 cgagaagccg gagaggacca ccggcgcggt tcgtcgagct ggacttgcgg gcgccgaagg
     1501 gacagtgaac gggccaggtc cagcgcgtct tcgcggcttt ggatctggc
//
LOCUS       DAOSPL010001314         1549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_80931_length_1549_cov_2.439759, whole genome shotgun sequence.
ACCESSION   DAOSPL010001314 DAOSPL010000000
VERSION     DAOSPL010001314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80931_length_1549_cov_2.439759"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /locus_tag="PLQ54_19305"
     CDS             complement(<1..1403)
                     /locus_tag="PLQ54_19305"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOS95468.1"
                     /translation="MASGRDEAQTVEAALDALRRLAKPLEWERRAGYRDSVAEGGLAA
                     YVARWAGEVSLRMGRPNVSAAVGEVREALAGYSSLDAAGREAAVSLAESLVRQTEEII
                     RAPDAIPEPQGTLRTDSPHRGLTLKDAAQTDVQWLKGVGPAVAEKLAALGIRTVYDLL
                     HYLPRAHEDRRSPPRVRELVDGEVAGVRVRLLGPPAARQTRRMPVVKALARDDTGMTT
                     LVWFNQHWVAERLRDGDVLIVSGRVSTRLGSSEIAVTEYEVEPPEGDSLGSGRIVPIY
                     GLTSGLYQNNLRRAVRRALDQFITSDFDPLPAAIRSRRRLLSYAEALHAAHYPTAPED
                     LEASHHRLAYEELLVLETELASRRFGMTRRQAPRLEHAAEAVRELARALPYELTGAQK
                     RVIMRIANEVTRPSPMQRLLHGDVGSGKTAVAMAAILMAVRSGYQAALMAPTEILAEQ
                     HLRTLTETLGPLGVSVVL"
BASE COUNT          250 a          585 c          483 g          231 t
ORIGIN      
        1 agaaccacac tcactccgag ggggcccagc gtctccgtca gggttcgtag atgctgctca
       61 gcgaggatct cggtgggcgc catcagtgcg gcctgatagc ccgatcgcac cgccatcaga
      121 atggcggcca tcgctactgc cgttttcccc gatcccacgt cgccatgcag caacctctgc
      181 atggggctgg gccgagtcac ctcgttcgcg atccgcatga tcacccgctt ctgggcgcca
      241 gtgagctcat agggcagggc ccgggccagc tcccggaccg cttcggccgc gtgctccagc
      301 cggggcgcct ggcgacgtgt cataccaaaa cggcgcgagg ccaactccgt ctccagcacc
      361 aacagctcct cataggccag cctatgatgc gaggcctcca ggtcctcggg agccgtgggg
      421 tagtgcgccg cgtggagggc ctcagcgtag ctcagaaggc gccgacgcga gcggatcgcc
      481 gctgggaggg ggtcgaaatc actggtgatg aactggtcca gcgcccggcg gaccgcgcgg
      541 cgaaggttgt tctgatacag gccactggtg aggccgtaga tggggacgat gcgcccggac
      601 cccagcgagt caccctccgg cggttcgacc tcatactccg tcacggcgat ctcggagctg
      661 cccaggcgcg tggacacccg tccgctgacg atcagcacgt cgccatcacg caagcgctcg
      721 gcgacccagt gctggttgaa ccacacgagc gttgtcatgc ccgtatcgtc acgcgccagg
      781 gccttcacaa ccggcatacg ccgagtctgc cgcgcggcag gcggccccag caaacgcaca
      841 cgcacgccgg cgacctcgcc atcgaccagc tccctgactc gtggcggaga gcgccggtcc
      901 tcatgcgccc gcgggaggta gtgcagcagg tcgtacaccg tgcggatgcc cagcgctgcc
      961 agcttctctg ccaccgccgg gcccacgccc ttcagccact gcacgtctgt ctgcgccgca
     1021 tccttgaggg tgaggcctcg atggggcgag tccgtccgaa gcgtcccctg aggttccgga
     1081 atcgcgtcag gcgcacggat gatctcctcg gtctggcgca ccaacgactc cgccagcgac
     1141 accgccgcct cgcggcccgc cgcatcgagg cttgagtagc cggcgagtgc ctcgcgcacc
     1201 tccccgacgg cggcggacac gttcggccgt cccatgcgga gcgagacctc gcccgcccac
     1261 cgcgccacgt aggccgccag cccaccctca gccacggaat cgcggtaccc ggcccgacgc
     1321 tcccactcca gcggcttggc aagccgtctc agtgcatcga gtgccgcttc aacggtctgt
     1381 gcctcgtcac gaccgctcgc catgttcgcc cccgggcgcc cggttccgcc gggcacctct
     1441 agacaaaggt gtcctcctcc cgcctgagcg cgcacaagcc cgcgtcaagg atcgcccacg
     1501 catagaggcc catgtcgagc cccccgagca ccccgatctg ccacaggcg
//
LOCUS       DAOSPL010001315         1546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_81124_length_1546_cov_4.153588, whole genome shotgun sequence.
ACCESSION   DAOSPL010001315 DAOSPL010000000
VERSION     DAOSPL010001315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81124_length_1546_cov_4.153588"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PLQ54_19310"
     CDS             complement(<1..747)
                     /locus_tag="PLQ54_19310"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOS95469.1"
                     /translation="MRRSSRCLPLLAPMATVAVAGLLSGCHCPPGPALPAAVVQSSLI
                     PTVPGVPMYHGATIAPLVGGDVLAAFYAGTEERHPDVAIYWSRYNRVAQAWSRPEALV
                     DNPGISEGNPVLGRAPDGRLWLFYVTLPERSWSVARMFSRFSSDEGATWSRPREMFFI
                     PGWIPRNPPIVLSSGDWLLPFYDSARHASLFSLSPDDGSTWTDPCVLSARGRCIQPTV
                     IERADGSLVALMRTTDPPHWLLRSTSTDGGH"
     gene            complement(855..>1546)
                     /locus_tag="PLQ54_19315"
     CDS             complement(855..>1546)
                     /locus_tag="PLQ54_19315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95470.1"
                     /translation="VDEVSRLNRAWGTSYGAFADIDPRDTMVFDRPAHIEGRAAHPQA
                     VEDHVEFCSQMVNRGLLAMKDRLRAKMPDLLIASELPYPVGSRHPHAVAYRIGCNANP
                     STAYHADVLFLRMTGPMDDVERQTLASHIRATGQKVVLCYRTYSDWGRPPAEGGADPT
                     VVSELYGFEPARFAHGIGFYSWNEMVDVHLSAPGQGSPINEMTVRPPVSDRMQAQAES
                     ILRAYRGVYGR"
BASE COUNT          265 a          497 c          539 g          245 t
ORIGIN      
        1 gtgaccgcca tcggtcgacg tggacctcaa cagccagtga ggcgggtcgg tggtccgcat
       61 tagggccaca agtgagccat ccgcgcgctc gatcacggtc ggctggatac accgacctcg
      121 tgccgacagc acacagggat cggtccacgt cgacccatcg tcgggcgaca ggctgaagag
      181 tgatgcatgt cgtgcactgt cgtagaacgg tagcagccaa tcgcccgacg agaggacgat
      241 gggcggattc cgcgggatcc agccggggat gaagaacatc tcgcggggcc gagaccacgt
      301 ggccccctcg tccgatgaga agcgggagaa catgcgcgcg accgaccagg accgctccgg
      361 cagcgtcacg tagaacagcc acaggcgccc gtcaggcgcg cgcccgagca ccgggttgcc
      421 ttcggagatg ccggggttat ccaccaacgc ctcgggtctc gaccaggcct gggccactcg
      481 gttgtaccgg gaccagtata tggcgacatc cggatggcgc tcttccgtgc cggcgtagaa
      541 ggcggccagc acgtctccgc ccaccagcgg ggctatggtg gcgccgtggt acatgggtac
      601 gcccggcacg gtggggatca gggagctttg caccacggcg gcggggagcg cgggcccagg
      661 cgggcagtgg catcccgaga gcagaccagc gaccgcaacc gtcgccatcg gagccaggag
      721 tggcaggcag cgggaggaac ggcgcatact gatcacccca cgaagcatcc tttccgcgtc
      781 gggtccggtc tggccttgca tccctcccag gttggcagca tccccgaagt tgggcgtgac
      841 gccgcgccgt ggcgctaccg cccatacaca ccccggtaag cgcgaaggat cgactcggcc
      901 tgcgcctgca tccggtcgga aacgggtggc cgcacagtca tctcgttgat cgggctgccc
      961 tggcccggcg ccgatagatg cacatcgacc atctcgttcc agctgtagaa gccgatgccg
     1021 tgcgcgaacc gggccggctc gaagccgtac agctccgaga ccacggtggg gtcggccccg
     1081 ccctcggccg gcgggcgccc ccagtcgctg taggttcggt agcagaggac gaccttctgg
     1141 cccgttgccc ggatgtggct ggccagggtt tgccgctcga cgtcgtccat gggcccggtc
     1201 atccgcagga agaggacgtc ggcgtggtag gccgtgctgg ggttggcgtt gcagccgatg
     1261 cggtatgcga cggcgtgggg gtgtctcgat ccgactgggt acggcaactc gctggcgatg
     1321 agtaggtcgg gcatcttggc ccggagccga tccttcatgg ccagcagccc tcggttcacc
     1381 atctgggaac agaactcgac atgatcttca acggcctgtg ggtgggccgc gcgtccctcg
     1441 atgtgtgccg gacgatcgaa caccatggtg tctctggggt cgatgtcggc gaacgcgcca
     1501 tatgatgtgc cccaggcacg attgaggcga gacacttcgt ccacgt
//
LOCUS       DAOSPL010001316         1546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_81144_length_1546_cov_3.067069, whole genome shotgun sequence.
ACCESSION   DAOSPL010001316 DAOSPL010000000
VERSION     DAOSPL010001316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81144_length_1546_cov_3.067069"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1546
                     /locus_tag="PLQ54_19320"
     CDS             <1..>1546
                     /locus_tag="PLQ54_19320"
                     /inference="COORDINATES: protein motif:HMM:NF026035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95471.1"
                     /translation="DGAMWPSISANGSTIVFEHANGLWTVPTKGGDPKELRMRAPIDY
                     PQTFESRKSVSSGITELEVAPDGKLLAVCVNGDILLLKPEFENDSIRLTDHPGNDSDF
                     MWSPDSKKLVFTSDRTGNSDLFIADAETLQVRQLTNTPEYEHSPRFNPKGDQVYFTRS
                     AADGLYSIPAEGGDIRQVHPGPFVEEYSFSPCGHWLALGLQDTVGTRDLWIVPAEGGE
                     AHNITKYPGGNYSPVWDPNGEYLFFVSTRTGNGDIYSVRLTKDAEKFDDYQAQEEEKA
                     KREGKKPAPPEKPAEDGEKKPNGGNDGETPETPKDEEAKPAEGEAKPAEEAQPEKEPI
                     AIDFDDIENRARRLTSTNEGEGNLTIAPKGDRLVYTVAPRGEPQVWQMKPDGKDAKPF
                     ISGNVAAGALRWTASGDRLYYSANGKPYYVGAGGGGGSEVSFRFEYVDDARVRQVAAF
                     NQAWMALDEHFYDGNFHGVDWKAIREKYAKMVDGTLVPGDFNIVVQRMIGELNASHLG
                     CYGGGIS"
BASE COUNT          327 a          463 c          532 g          224 t
ORIGIN      
        1 gatggcgcga tgtggcccag catctcggcc aacggcagca ccatcgtgtt cgagcacgcg
       61 aacggcctct ggacggtacc gaccaagggg ggggacccca aggagctgcg gatgcgggca
      121 ccgattgact atccgcagac attcgagtcc cgcaagagcg tgagctcggg cattaccgag
      181 ctggaggtgg cgccggacgg gaagttgctg gccgtgtgtg tgaacggcga catcctgttg
      241 ctcaaacccg agttcgagaa tgactcgatc cggctgacgg accacccggg gaacgactcg
      301 gacttcatgt ggtcgcccga cagcaagaag ctggtgttca cctcagatcg caccggcaac
      361 agcgacctgt tcattgcgga tgccgagacg cttcaggtgc ggcagctcac gaacacgccc
      421 gagtacgagc attcgccccg gttcaacccg aagggcgacc aggtctactt cacgcgttcg
      481 gcggccgacg ggctgtattc gatcccggcc gaggggggcg acatccggca ggttcaccca
      541 ggccccttcg tcgaggagta cagcttctcg ccctgcgggc actggctggc cctggggctg
      601 caggacactg tcggcacgcg ggacctgtgg atcgtgcccg cagagggcgg cgaggcccac
      661 aacatcacga agtatcccgg cggcaactac tcacccgtgt gggatcccaa cggcgagtat
      721 ctgtttttcg tctcaacccg cacgggtaat ggggacatct actccgtgcg cctgaccaag
      781 gatgccgaga agttcgacga ttaccaggct caggaggaag agaaggccaa gcgagagggt
      841 aagaagccgg cgccgccaga gaagccggcc gaggatgggg agaagaagcc gaacggtggg
      901 aacgatgggg agacgccgga gacccccaag gacgaggaag ccaagccggc cgagggcgag
      961 gcaaagccag ccgaggaggc ccaacccgag aaggagccca tcgccatcga cttcgatgac
     1021 atcgagaacc gtgctcggcg gctcacgtcg accaacgagg gcgaaggcaa cctgaccatc
     1081 gcccccaagg gagaccgact ggtctacacc gtggcacccc gcggcgaacc gcaggtgtgg
     1141 cagatgaagc ccgacgggaa ggacgcgaag ccgttcatca gcggaaacgt cgcggctgga
     1201 gcgctgcggt ggaccgcaag cggcgatcgg ctgtactact cggcgaacgg caagccctac
     1261 tacgtcggcg caggtggtgg cggcgggtcg gaagtcagtt tccggttcga gtatgtcgac
     1321 gatgcgcgcg tccgccaagt ggcggccttc aaccaagcgt ggatggcact ggacgagcac
     1381 ttctatgacg gcaacttcca cggtgtggac tggaaggcca tccgcgagaa gtacgccaag
     1441 atggtggatg gcaccctggt gcccggtgac ttcaacatcg tggtccagcg catgatcgga
     1501 gagctgaacg catcgcacct ggggtgctac gggggcggca tcagtg
//
LOCUS       DAOSPL010001317         1543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_81365_length_1543_cov_3.069220, whole genome shotgun sequence.
ACCESSION   DAOSPL010001317 DAOSPL010000000
VERSION     DAOSPL010001317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81365_length_1543_cov_3.069220"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PLQ54_19325"
     CDS             <1..360
                     /locus_tag="PLQ54_19325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95472.1"
                     /translation="RLDAGAGRVSSWYGQPISGGYLKPSLTYRFRIADDALPGLWPFL
                     YLDRIEGRITYQFIRNFGAPLYEADGHTLLAEIENRGYITRALPYSLRVGAYVDTRNP
                     SLAPVPYARFSFNVASF"
     gene            complement(362..1249)
                     /locus_tag="PLQ54_19330"
     CDS             complement(362..1249)
                     /locus_tag="PLQ54_19330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOS95473.1"
                     /translation="MKPNRPAASDPKAVAEEHYRLMLAARQAALHTQLLREAIVEVGE
                     RRRREQRLVQRLQEQYLQLEALNAQKDELLGMAAHDLRNPLSVVLGFADVLLADPAVS
                     GNEQLREFGTIIADTARHMVALIDDILAVSQIESGRLTIDREPADLAALVRAACVPNR
                     MLAERKSITLLEHLPAHPVITRIDPLRIRQVLDNLLSNAIKFSYERTTITVRVWVADD
                     RAFIEVADQGQGIRDEDLGKLFRPFTQTATRATAGEKSTGLGLAIVKRIVELHGGTVN
                     VHSRWGQGATFTIALPLVT"
     gene            complement(1246..>1543)
                     /locus_tag="PLQ54_19335"
     CDS             complement(1246..>1543)
                     /locus_tag="PLQ54_19335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95474.1"
                     /translation="GFARQLDLFAGGDYAERAAGFHRAYMPDSALLQRTLALFRALAP
                     ATLAPQHGAVCRGEIVDAVLARLAEVDCGIARSEPWYPESLRTVAESIQGDSSQ"
BASE COUNT          232 a          482 c          553 g          276 t
ORIGIN      
        1 cgcctggatg cgggggcggg ccgggtgtcg agctggtatg gtcagccgat ctcgggggga
       61 tacctgaagc cgagcctgac gtaccgtttc cgcatcgcgg acgatgcgct gccgggcctg
      121 tggcctttcc tgtatctgga tcggatcgag gggcgcatca cctaccagtt tatccggaac
      181 ttcggcgcac cgttgtatga ggcggacggc cacacgctcc tggccgagat cgagaaccgc
      241 ggctacatca cgcgagccct gccctacagc ctgcgcgtgg gggcgtatgt cgatacgcgg
      301 aacccgagct tggctcccgt gccctatgcc cgcttctcgt tcaacgtggc atcgttctga
      361 gtcaggtcac gagcggcagg gcgatggtga atgtggctcc ctgaccccac cggctgtgca
      421 cgttcacggt cccaccgtgc agctccacga tgcgcttcac gatggccaag cccaagccgg
      481 tgctcttctc gccggccgtg gcacgggtcg cggtctgggt gaagggccgg aacagcttcc
      541 cgaggtcctc gtcgcggatg ccctgtccct ggtcggccac ctcgatgaag gcccggtcat
      601 cggccaccca gacgcgcacc gtgatggtgg tccgctcata tgagaacttg atggcgttac
      661 tcagcaagtt gtcgaggacc tggcggatcc gcagtggatc gatccgggtg atcacggggt
      721 gagcgggaag gtgctcgagc agagtgatgc tcttgcgctc ggccagcatg cggttcggga
      781 cgcaggcggc gcgcacgaga gcggccaggt cggcgggttc gcggtcgatg gtcaggcgcc
      841 ccgattcgat ctgtgacacg gccaggatgt cgtcgatcag cgccaccatg tggcgggcgg
      901 tatcggcgat gatggttccg aactcccgta actgctcatt cccactgacc gcgggatccg
      961 ccagcagcac gtcggcgaag cccaggacga ctgaaagagg gttccgcaga tcgtgggcgg
     1021 ccattccgag gagctcgtct ttctgggcgt tcagcgcctc gagctggagg tactgctcct
     1081 ggagtctctg tacaagccgt tgctcgcggc ggcggcgttc gccgacctcg acgatggcct
     1141 cgcgcagcag ttgcgtgtgc agggccgcct ggcgggcggc gagcatcagg cggtagtgtt
     1201 cttcggcgac tgccttgggg tctgaagcgg ccggccggtt cggtttcact gcgaggagtc
     1261 tccctggatg ctctcagcga ccgtccggag cgactcgggg taccatggtt cggaccgagc
     1321 gatcccacag tccacctcgg cgagtctcgc caggaccgcg tccactatct cgccgcggca
     1381 gacggcgccg tgttgggggg cgagggtggc tggcgcgagt gcgcgaaaca gggccagcgt
     1441 gcgctggagc agggccgagt cgggcatgta ggcgcggtgg aacccggcgg cgcgctcggc
     1501 gtagtcgccg ccggcgaaca ggtccaactg gcgggcgaac ccg
//
LOCUS       DAOSPL010001318         1542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_81442_length_1542_cov_3.009415, whole genome shotgun sequence.
ACCESSION   DAOSPL010001318 DAOSPL010000000
VERSION     DAOSPL010001318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81442_length_1542_cov_3.009415"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="PLQ54_19340"
     CDS             complement(<1..1055)
                     /locus_tag="PLQ54_19340"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOS95475.1"
                     /translation="MGKIRVLHIITRLIVGGAQEMVLLAARGVDSHRFECHVVAGPET
                     GPEGSMQDEFHAAGVRVTIEPDLCREMSPLRDWRATRALSRLIGDGGYSLVHTHTAKA
                     GILGREAARRAGVPAVHHVHGWSFSGFRGWRRRVFAALERRAARSSAALAVVTDRDIR
                     KGLDAGIGHIEQYRLIRAGVDLSRFPLAGPEERRAARARLGIAPDEFCVGYVGRLAPQ
                     KAPLDLVRAVAAARQHGQLRFLLVGDGPMRAETERALHAARLTDSTIQLGLRRDIEAV
                     LPACDVFASASLWEGLPRALIEAMAVGVPVAATAVDGVAELIVNRETGLLVPPGDPEA
                     LGRAITELEDARPGDFL"
     gene            complement(1062..>1542)
                     /locus_tag="PLQ54_19345"
     CDS             complement(1062..>1542)
                     /locus_tag="PLQ54_19345"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOS95476.1"
                     /translation="RTHRQRPQLGDAVASHRLELEFHEPLAHVAAGRALAESGVVSAM
                     MDVSDGLAGDATKLASASGVGVCVDARKIPLSPDLVACYGTPGALSLALQGGEDYALI
                     FTVPPERVAQAEEALARVHYTAAAIGTVVSQDQGCTFLDRSGHSHPLATADSWDHFR"
BASE COUNT          250 a          535 c          554 g          203 t
ORIGIN      
        1 ctcaggaagt cgcccggccg cgcatcctcc agttcggtga tcgcccggcc gagggcctcc
       61 gggtcgcctg gcgggaccag cagcccggtc tctcgattca cgatcagttc cgcgacacca
      121 tccacggccg tggcggccac ggggaccccg acggccatgg cctcgatcag cgcccgaggg
      181 agtccctccc acagcgaagc ggaggcgaag acgtcgcagg ccggaagcac cgcctcgatg
      241 tcgcgacgca ggcccagttg gatggttgag tccgtcagcc gagcggcatg cagagctcgt
      301 tcggtctctg cacgcatggg gccatcgccg accagcagga agcgaagctg cccatgctga
      361 cgcgcggcgg ccacggcacg gaccaggtcc aggggcgcct tctggggcgc cagacggccc
      421 acatagccca cgcagaactc gtcgggtgcg atgccaagcc gagcgcgcgc agctcggcgc
      481 tcctcggggc cggcgagggg gaagcgggac aggtccacac cggcccggat gaggcggtac
      541 tgctcgatgt ggccaattcc ggcatcgagt cctttgcgga tgtcgcggtc cgtgacgacg
      601 gcgagcgcgg ccgaactgcg tgcggcgcgc cgctcgagtg ccgcgaagac ccgcctccgc
      661 cagccccgga agccactgaa gctccagcca tgcacgtgat ggaccgcggg aacgcccgcc
      721 cggcgagcgg cctcgcgccc gaggatcccc gccttggcgg tgtgcgtgtg caccagcgag
      781 tagccgccgt cgccgatgag gcgggacagg gcacgcgtgg ctcgccagtc gcgcagcggc
      841 gacatctcgc ggcacagatc gggttcgatg gtgacccgga cgccggcggc gtggaactcg
      901 tcctgcatgg agccctcggg tccggtctcg ggaccggcga cgacgtggca ctcgaaacgg
      961 tgggaatcca cgccgcgagc tgccagaagc accatctcct gggcgccgcc cacgataagc
     1021 cgggtgatga tgtggagtac gcggatcttc cccatgggag cctaccggaa gtggtcccac
     1081 gagtccgctg tagccagcgg gtgactatgc cccgagcggt ccaggaaggt gcatccctgg
     1141 tcctgcgaca cgacggtgcc gatcgccgcg gcggtgtagt gaacacgggc cagggcctct
     1201 tcggcctgcg ccacgcgctc gggcggaacg gtgaagatca gggcgtagtc ctcaccgccc
     1261 tgcagagcga gcgagagggc accgggtgtg ccgtaacagg ccaccaggtc aggggagaga
     1321 gggatcttcc gcgcgtccac acagacaccc acgcccgacg ccgaggccag cttggttgca
     1381 tcgcctgcca gcccgtcgct gacgtccatc atggcactga cgacgccgga ctcggccaga
     1441 gcgcgacccg ccgccacgtg tgccagcggc tcatggaact cgagttcgag ccggtgcgag
     1501 gccacggcgt cgcccagctg cgggcgttgg cggtgggtgc gg
//
LOCUS       DAOSPL010001319         1540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_81581_length_1540_cov_3.230976, whole genome shotgun sequence.
ACCESSION   DAOSPL010001319 DAOSPL010000000
VERSION     DAOSPL010001319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81581_length_1540_cov_3.230976"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1256
                     /gene="alr"
                     /locus_tag="PLQ54_19350"
     CDS             120..1256
                     /gene="alr"
                     /locus_tag="PLQ54_19350"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOS95477.1"
                     /translation="MRPAWVEVDLDALRANLQSIRQVVGLECELMAVVKANAYGHGLV
                     RVAQHFVACGADSLCVALLEEALELRESGVAAPILVMGAMVPDQASDYVTHGITASLC
                     SEDGLKALSSACEAQGKSAAVHLKVNTGMNRIGVSPSAVSALGQAVRDDSHLVLGAVW
                     SHLATADESDDSFAREQHRRLMAAVRELRSVGVDVPMVHLAASAGIARFGEMHLGGVR
                     PGLALYGMDPCPGHNVFGIHYRPALSVKARIVALTDVDRGDGVSYGLTHEAAGPSRIA
                     TVPLGYADGYDRRFSNSGMVVVRRRVVPVVGRVCMDQFMVDVTGVPDVVIGDEVLVAG
                     GPEPEMSVCALAKRLGTIPHEFVTGLSQRLPRVYRGASLDGGSP"
     gene            1366..>1540
                     /locus_tag="PLQ54_19355"
     CDS             1366..>1540
                     /locus_tag="PLQ54_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOS95478.1"
                     /translation="MRSRQTVGAARLSVASNIVLVVAKLVVGWVTGSVSVVAEALHSG
                     NDLLAAAIALGAVR"
BASE COUNT          222 a          454 c          580 g          284 t
ORIGIN      
        1 ctgagtaggt ggcaagtcta tcatggcaga gggcgatgca ccaagcagct cgagttgacg
       61 ctccgtgtgg ggtagcccta tgatggccgg gttacccttc ccgtcctcgg agagccgcca
      121 tgcgtccggc ctgggtcgaa gtcgacctcg acgcccttcg tgcgaatctt cagtccatcc
      181 gccaggtcgt tgggcttgag tgcgagctca tggccgtggt caaagccaat gcgtatggtc
      241 acggtctggt gcgagtcgcc cagcacttcg tggcgtgcgg cgcggactcg ctgtgcgtcg
      301 cgttgttgga ggaggccctg gagctccgag agagcggggt tgcggcgccg attctggtga
      361 tgggggcgat ggtgcccgat caggcgtcgg actacgtcac ccatggaatc acggcctccc
      421 tgtgcagtga ggacggactg aaggcgctct cctccgcgtg tgaggcgcag ggcaagagcg
      481 cggccgtcca tctaaaggtg aacacaggca tgaaccggat cggggtttcg ccgtcggccg
      541 tgtcggccct gggtcaggcg gtacgcgatg actcccacct ggtgctgggc gctgtgtggt
      601 cccacttggc cacggcggac gagtcggacg actcattcgc ccgggagcag caccggcggc
      661 tcatggctgc cgtgcgggag cttcggtccg tgggagtgga cgtgccgatg gtccacctgg
      721 cggcgagcgc gggcatcgct cgtttcgggg agatgcacct ggggggggtt cggccaggcc
      781 tggcgctgta cgggatggac ccgtgcccgg gccacaacgt gttcgggatc cactaccgcc
      841 cggcgctgtc agtgaaggcg aggatcgttg cactcacgga tgtcgatagg ggtgacggag
      901 tttcatacgg cctgacccat gaggctgcgg gtccgagccg gattgccaca gtgcccctcg
      961 ggtacgccga cggctacgac cggcgtttct ccaacagtgg tatggtggtt gtcagaaggc
     1021 gcgtggtccc tgtggtgggc cgcgtctgca tggaccaatt catggtggac gtcacaggag
     1081 tgccggacgt ggtgatcggc gacgaagtgc tcgttgccgg cggaccggag cccgagatgt
     1141 cagtctgcgc actggcgaag cggctcggga ccatacccca cgagtttgtc acggggctga
     1201 gccagcggct cccacgtgtg taccggggcg cttccctcga tggcgggtcg ccgtagatgg
     1261 ctgccggggc gggatctaga ggtggccaga gccgtcggcg cgcgtctgga ggaggcccgc
     1321 gtggctgagg ctggagccaa ctcgcacgcc ggggacccat cgctgctgcg gtcgcgccag
     1381 accgtgggtg cggcacggct ttcggtggcc tcgaacatcg tgctggtcgt cgccaagctg
     1441 gtggtgggct gggtcacggg ctcggtgagt gttgtggcgg aagcgttgca ctcaggcaac
     1501 gacctgctcg ccgccgccat cgccctgggt gccgtgcgca
//
LOCUS       DAOSPL010001320         1540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_81591_length_1540_cov_2.746801, whole genome shotgun sequence.
ACCESSION   DAOSPL010001320 DAOSPL010000000
VERSION     DAOSPL010001320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81591_length_1540_cov_2.746801"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1540)
                     /locus_tag="PLQ54_19360"
     CDS             complement(<1..>1540)
                     /locus_tag="PLQ54_19360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95479.1"
                     /translation="VTAVVRYIKTQLRRGRPDAPPEALALVAGGERTTESLAATVSGW
                     TCRRIAAYLFRRLLARAGQALRSMEHRAPGVQTEAATLCASVPTASVGEGEPYQEWIR
                     VAAQDAKPRGPIQMVPEPECAAMDLLASWGTYFPDRQHELQRPTPMTVMVFDCGAGTT
                     DVALLVVEFSGARPAIASRASAGYEFSGNLVDYALTREIVQNYWALAGPADASGSALR
                     VPAFDDPALGSYEIERPGPRGAGGREALVSFLALQDTVRQAKERWPALGPRSLSVRIY
                     EREVTLDDEAISRVIREQLLGVVDGGYSPRSYFRAGLGLRSVVDQALEELGREGDYPL
                     EYIILTGGSSQLREVRQVLGERFLGAADDPRVLMPGPEHLRVNVAKGATRAATFFVPG
                     TMPLDIRARMTLHSPGLPDEVWEAPVFATGSPPHAVELRGSPAEGWTRNGVDARTGRF
                     VITQGTTALVEVLAANPRPEAASDAAWRLHSPPLAVTYRAPDGLPAGALAGVALAVRY
                     TAHNA"
BASE COUNT          252 a          549 c          534 g          205 t
ORIGIN      
        1 tgcgttgtgg gccgtatacc gaacagccag agcgacgcct gcaagcgcgc cggccggtag
       61 cccatcggga gcccggtagg tcaccgccag tggcggcgag tgaaggcgcc acgccgcatc
      121 gctggctgcc tcgggccggg gattggcggc caggacctcc accagagccg tggtgccctg
      181 ggtgatgaca aacctgccgg tgcgggcatc gacgccgttt cgcgtccagc cctcagcggg
      241 gctgccccgg agctcgaccg cgtgaggagg cgaacctgtc gcgaacacgg gcgcctccca
      301 gacctcgtcg ggcaagcccg gggaatgcag ggtcatgcgt gcgcggatgt cgagcggcat
      361 ggtccccggc acgaagaagg tggccgcgcg cgtcgcgccc ttggccacgt tgacccggag
      421 gtgctcgggg ccgggcatca ggacccgagg atcatccgcc gccccgagaa accgttcgcc
      481 gagcacctgg cgcacctccc ggagctgact gcttccgccg gtgaggatga tgtactcgag
      541 tgggtagtcc ccctcgcggc caagctcctc cagggcttga tcgaccaccg aacgcagccc
      601 aaggcccgcc cggaagtacg agcgagggct gtagccccca tctacaacgc cgagaagctg
      661 ctcgcgaatc acccgcgaga tggcctcatc atcgagggta acctcccgtt cgtagatgcg
      721 gacggacagc gagcgcgggc ccaaggcggg ccaccgctcc ttcgcctgcc ggaccgtgtc
      781 ctggagcgct agaaaggaga ccagcgcctc acgacccccg gcgcccctcg ggccaggcct
      841 ctcgatctcg tagctgccca gcgcgggatc gtcgaaagcc ggaacgcgca gggccgaccc
      901 ggacgcatcc gccggcccgg ccagagccca gtagttctgc acgatctcgc gcgtcagcgc
      961 atagtccacc aggttacccg agaactcgta ccccgcggag gcccgggagg cgatggcagg
     1021 gcgggcgcct gagaactcca ccacgaggag cgccacatcg gtcgtgcccg ccccgcagtc
     1081 gaagaccatg accgtcatcg gggtggggcg ctgcagctcg tgctggcggt cggggaagta
     1141 ggtgccccaa gaagccagga ggtccatggc cgcacactcg ggctccggca ccatctggat
     1201 ggggccgcga ggcttcgcat cctgagccgc cacccgtatc cactcctggt agggttcgcc
     1261 ctcacccacc gatgcggtgg ggacactcgc gcacagagtg gccgcctcag tctgcacgcc
     1321 gggggcacgg tgctccatgc ttcgcagcgc ctgcccggca cgggccagca ggcggcgaaa
     1381 gaggtaggcc gcgatccgcc gacacgtcca tccggacacg gtcgcggcca gggactcggt
     1441 tgttcgctct cccccggcaa ccagcgccag agcctcgggg ggcgcgtcgg ggcgcccccg
     1501 gcggagctgt gtcttgatgt atcggaccac cgcggtgacg
//
LOCUS       DAOSPL010001321         1539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_81690_length_1539_cov_1.882075, whole genome shotgun sequence.
ACCESSION   DAOSPL010001321 DAOSPL010000000
VERSION     DAOSPL010001321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81690_length_1539_cov_1.882075"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PLQ54_19365"
     CDS             <1..642
                     /locus_tag="PLQ54_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoD"
                     /protein_id="HOS95480.1"
                     /translation="CVAHDLPEQFLPLARDLVSNRLPRALDQLDTLLTHNEIVKLRSQ
                     GVGVLPRDIALSLSCSGPMLRASGVNYDLRRADPYSIYDRFDFKVITRTEGDTYARYL
                     LRMDEARQSLRILEQALKQIDDTPPGDILAGRKNWQLRPPKGEVYGRAENPKGELGFY
                     IVSDGTANPYRYHVRSPAFINLAALNEMCRGHKVADVVAIFGSIDTTMGEVDR"
     gene            639..1223
                     /locus_tag="PLQ54_19370"
     CDS             639..1223
                     /locus_tag="PLQ54_19370"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOS95481.1"
                     /translation="MMTGLGVVRGMLVTLWHFILSYVEDVKRFPRRYRDDRASVNQGV
                     ESRGVFTVQYPEERLRMFERFRSFPVLIYETETGEERCTACGICAKVCPPQCIWIVRA
                     KTPEGKPKPKPEEFVIDISICMSCGLCAEFCPFDSIKMDHKYELAGYERLSTYVLDKK
                     ALLVSSDYYAQTHPIAAAEEDEARRKKAEAKAGR"
     gene            1285..>1539
                     /locus_tag="PLQ54_19375"
     CDS             1285..>1539
                     /locus_tag="PLQ54_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOS95482.1"
                     /translation="MAYVICQPCINTKDTACVAVCPVDCIHPTKDEAAYATVEQLYIN
                     PDDCIDCGACAPECPVDAIFSGDEVPEQWKDFAAKNAAYYK"
BASE COUNT          312 a          493 c          463 g          271 t
ORIGIN      
        1 tgcgtggcgc acgacttgcc cgagcagttc ctgcccctgg cacgcgacct cgtcagcaat
       61 cgtctgccca gggcattgga ccagctcgac acactcctga cccataacga aatcgtcaag
      121 ctgcgttcac agggcgtggg tgtgctgcca cgcgacatcg cgctcagcct cagctgctcg
      181 ggccccatgc tgcgggccag cggcgtcaac tacgacctgc gacgggccga cccctattcg
      241 atctacgacc gctttgactt caaggtgatc acccgtaccg agggcgacac ctatgcccga
      301 tacctgttgc gcatggacga ggcgcggcag agcctgcgca ttctcgagca ggctctcaag
      361 cagatcgacg acaccccgcc cggcgacatc ctggcggggc gcaagaactg gcagctcagg
      421 ccgcccaagg gagaagtcta cggacgggcc gagaatccaa agggtgaact cggcttctac
      481 atcgtgtcgg acggtacggc caatccctac cgctatcacg tccgctctcc tgcattcatc
      541 aacctggccg ctctcaacga gatgtgccgc gggcacaagg tcgccgacgt tgtggccatc
      601 ttcggcagca tcgacaccac aatgggggag gtggaccgat gatgaccgga ttgggcgttg
      661 tccgtggcat gctggtaacg ctgtggcact tcattctcag ctacgtggag gatgtgaagc
      721 gcttcccgcg gcgctaccgc gatgatcgag cgtcggtcaa ccagggcgtc gagtcgcgcg
      781 gcgtgttcac cgtgcagtac cccgaagagc ggctacgcat gttcgagcgc ttccgctcgt
      841 tccctgtcct gatctatgag acggagacgg gtgaggagcg gtgcacagcc tgcggcatct
      901 gcgccaaggt ctgcccgccg cagtgcatct ggattgtgcg cgccaagacg cccgaaggca
      961 aaccgaagcc caagccggag gagttcgtca tcgacatctc gatctgcatg tcatgcgggt
     1021 tgtgcgctga gttctgcccg ttcgattcga tcaagatgga tcacaagtat gagctggcag
     1081 gctatgagcg cctgtcgacg tacgtgctgg acaagaaggc gctgctcgtg tcgagcgact
     1141 actacgcgca gacgcaccca atcgcagcag ccgaggaaga cgaagcgcgc cgcaagaagg
     1201 ccgaagccaa ggccggccgc tgaggatcac tcgagactga tattccgcaa gatcgtttca
     1261 atactatgag gaggttccac accaatggca tacgtcattt gccagccctg catcaacacc
     1321 aaggacacgg cgtgcgtcgc cgtctgcccg gtcgactgca tccatcccac caaggatgag
     1381 gcggcctacg ccacggttga gcagctctac atcaaccccg acgactgcat cgactgcggc
     1441 gcctgcgcac ccgagtgccc ggtggacgcc atcttctccg gtgatgaagt cccggagcag
     1501 tggaaggact tcgcggccaa gaacgctgcc tactacaag
//
LOCUS       DAOSPL010001322         1539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_81696_length_1539_cov_1.609838, whole genome shotgun sequence.
ACCESSION   DAOSPL010001322 DAOSPL010000000
VERSION     DAOSPL010001322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81696_length_1539_cov_1.609838"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..638)
                     /locus_tag="PLQ54_19380"
     CDS             complement(126..638)
                     /locus_tag="PLQ54_19380"
                     /inference="COORDINATES: protein motif:HMM:NF014907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95483.1"
                     /translation="MPTDHAEFKAAEAAARERFAGYLAIGGTQSPDAFHMKLGRIMWD
                     YCGMERTKEGLEKALAEIPALHEEFRKDLRVTGGGTSTNQTLEKAGRVDDFFELAQVM
                     CLDALTREESCGGHFRAEHQTEEGEAERDDEHFAHVAAWEWTGDPSAPIRHTEPLEFD
                     NVHLAVRSYK"
     gene            691..891
                     /locus_tag="PLQ54_19385"
     CDS             691..891
                     /locus_tag="PLQ54_19385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95484.1"
                     /translation="MRVAYGTLELQRLDLCTVVDTGKVKVRLNGENVPFTLSQAGVGC
                     AIVFAEPLLLQVGDTLRVRMLS"
     gene            981..>1539
                     /locus_tag="PLQ54_19390"
     CDS             981..>1539
                     /locus_tag="PLQ54_19390"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOS95485.1"
                     /translation="MEEIKPLLRRAEPVKWLFYGDSITHGAKHTYGERDYTQLFAERV
                     RFELGRSHDIILNTAISGNTTWDLLASFDWRVAQFQPDVVFLMIGMNDHNRPGIITPE
                     EFGQNLGELADRVAVWGGRTVLQTTCPVLPSQPPEQIARFDCFMDEMRAVAAQRGLPL
                     VDHARYWADHADHHYFWMNNPSHPNA"
BASE COUNT          246 a          489 c          531 g          273 t
ORIGIN      
        1 gaggtcgagc atctcgagga acgacatctc gtcgttgatc cccggcgccg ccaccacgtc
       61 gaagcggccc ggcccggtgg ggccgtcctg ccgccagatc ttgagggtga ggttcagttc
      121 cttgctcact tgtagctccg gacggcgagg tggacgttgt cgaactcgag gggctcggtg
      181 tggcggatgg gcgccgacgg gtcgcccgtc cactcccagg ccgccacgtg ggcgaagtgc
      241 tcgtcgtcgc gctcggcctc gccctcctcg gtctggtgct cggcccggaa gtggccgccg
      301 caggactctt cgcgggtgag ggcgtcgagg cacatcacct gggccagctc gaagaagtcg
      361 tcgacccggc ccgccttctc gagggtctgg ttggtggagg tgccgccgcc ggtgacccgg
      421 aggtccttgc ggaactcctc gtgcagggcc gggatctcgg cgagggcctt ctcgaggccc
      481 tccttggtgc gctccatgcc gcagtagtcc cacatgatgc gaccgagctt catgtggaag
      541 gcgtcgggcg actgggtgcc gccgatggcg aggtagccgg cgaagcgctc gcgggccgcc
      601 gcctcggccg ccttgaactc ggcgtggtcg gtgggcaccg gctggggtac ctatcggcag
      661 cgcgtcagcg cgaaaaagac cgcctgcgcg ttgcgcgtgg cgtatggcac gctggagctg
      721 cagcggctgg acctgtgcac cgtagtggac acgggcaagg tcaaggtccg cctgaatggg
      781 gagaatgtgc cctttacgct gagccaggcg ggcgttggct gcgccatcgt gtttgctgag
      841 ccgctgctgc tacaggtggg cgacacgctg cgcgtgcgga tgctgtcatg atgtacgccc
      901 gagtctggca tgtgctctcc cgtctggcgc ggcgacgcca tacctcgcca atcagcgctt
      961 acatcgaagg gccgaaaaga atggaagaga tcaagccatt gctgcggcgg gcagaaccgg
     1021 tcaagtggct gttctacggc gacagcatca cgcacggggc caagcatact tacggtgagc
     1081 gcgattacac acagctcttc gccgagcggg tgcgtttcga gctggggcgc agccacgaca
     1141 tcatcctgaa caccgccatc agcggcaaca ccacatggga cctgctggcc agctttgact
     1201 ggcgcgtggc gcagtttcag ccggacgtgg tgttcctgat gatcggcatg aacgaccaca
     1261 accgccccgg catcatcacc ccggaggaat tcgggcagaa tctcggcgag ctggccgatc
     1321 gcgtcgctgt ctggggtggg cgcaccgtat tgcagacgac atgccctgtg ctgccgtcgc
     1381 agccaccgga gcagatcgcc cgctttgact gcttcatgga cgagatgcga gcggtggcgg
     1441 cgcagcgtgg cctgccgcta gtggatcacg cacgctactg ggccgaccat gccgatcatc
     1501 actacttctg gatgaacaac ccgtcgcatc ccaacgcct
//
LOCUS       DAOSPL010001323         1535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_81930_length_1535_cov_4.210135, whole genome shotgun sequence.
ACCESSION   DAOSPL010001323 DAOSPL010000000
VERSION     DAOSPL010001323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81930_length_1535_cov_4.210135"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..462
                     /locus_tag="PLQ54_19395"
     CDS             190..462
                     /locus_tag="PLQ54_19395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95486.1"
                     /translation="MDDAKWFALSFIAHGGSVREAAAWSGVGIPTIRRWLPSSRYGTV
                     GEAGAARRALRVMNLAPLDQSALVERMRRELSERESAAGPGETLPL"
     gene            613..>1535
                     /locus_tag="PLQ54_19400"
     CDS             613..>1535
                     /locus_tag="PLQ54_19400"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOS95487.1"
                     /translation="MRSTILTGLLALATAAAVAQAPPAAAPGDGPYRLAPGDVIDVVV
                     AGAPDPAYSPPADGIIIGPDGTIMYPTIGLVAAAGKTCEELASALALGLAARIRNPQV
                     VIRVREYNSLRFSVIGAVKRPGQFTFRQGITLREALALAEGVVLAGPGKASGARARLV
                     HQNGTSRSVSLENALRGLGDEGTLEIQVGDTLVVEQESSVSVLGYVRAPGYYQIPDGA
                     TLSEAIAMAGGVLEESGDQRRLIGDLAQVTVNRASGEVLTVDLLQAVQAPSEAGAAPA
                     DRAANNPVLESGDLVYVPPAKREASILGYVRT"
BASE COUNT          236 a          513 c          544 g          242 t
ORIGIN      
        1 gttcgccaac cccaggttgc gctcttccct cagcgaaccg tctcggccgg gggcatcggc
       61 gacgctctcg tcgcgtacgg cgtccccggc gccggctgtc cacccagccc gatctgtcgg
      121 cgcagacagg gaacccggcc atgaagaccg aaactggtac gcgcgcggtg ccggagaggg
      181 atcctacagg tggacgatgc caagtggttt gcgctgagtt tcatcgccca cggaggcagc
      241 gtccgggagg cggcggcgtg gtcgggtgtg gggatcccga caatccgccg atggcttcct
      301 tcgtcgcgct atggcactgt cggagaagcc ggcgcggcgc gcagggcact ccgggtgatg
      361 aacctcgctc cgctggacca gtcggccctg gtggagcgaa tgcgccgcga gctgagtgag
      421 cgagagagtg cggcggggcc cggagagacc ctgccactct agcgcggtct gacgatgcgt
      481 ggccagcggg aacggcacgc atcgcgcgac agggaaccgc aacgggctaa gccctggccc
      541 tcgggcccgt ggcggccagc gtgccggcgg gtctgacgcc cgccaccggc ttcgcgccaa
      601 tggagagaca cgatgcgctc aacgattctg acaggcctct tggcgttggc gacagcggcc
      661 gcggtggccc aagctcctcc ggccgctgcc cctggcgatg gtccttatcg gctcgcgccc
      721 ggcgatgtga tcgacgtggt agtcgccgga gcgccggacc ccgcctactc gccgcccgca
      781 gacggcatca tcatcggccc cgatggaacc atcatgtacc ccacgatcgg gcttgtggca
      841 gccgcgggga agacgtgtga ggagttggcg tcggctctgg ccttgggcct ggccgcgcgg
      901 atccgcaacc cgcaggtcgt cattcgcgtt cgcgagtaca actcgctccg gttcagcgtg
      961 atcggcgcgg tcaaacgccc cggacaattc acattccggc agggcatcac gctgcgagaa
     1021 gccctggcct tggccgaggg cgtggtgctg gctggtcccg ggaaagcctc tggagcgcgc
     1081 gcccggctgg tccaccagaa cggcacatcg cgaagcgtca gtctcgagaa cgccctgcgc
     1141 ggccttggcg acgagggtac cctcgagatc caggtcggcg atacgttggt cgtcgagcag
     1201 gagtcgtcgg tctccgtctt gggatatgtc cgggccccgg ggtactacca gatccccgat
     1261 ggcgccacgc tctcggaagc cattgcgatg gcggggggcg tcctcgagga atccggcgat
     1321 cagcgccgcc tcattggtga cctggcccag gtcacggtga atcgggcttc gggcgaagtg
     1381 ctcaccgtcg acctgctcca agcggttcag gcgccatcgg aagcgggcgc ggctccggcc
     1441 gatcgcgcgg cgaacaaccc ggtcctcgaa tcgggggatc tcgtctacgt gccgccggcc
     1501 aagcgagagg ccagcattct gggctatgtg cgcac
//
LOCUS       DAOSPL010001324         1535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_81936_length_1535_cov_3.786486, whole genome shotgun sequence.
ACCESSION   DAOSPL010001324 DAOSPL010000000
VERSION     DAOSPL010001324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81936_length_1535_cov_3.786486"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLQ54_19405"
     CDS             complement(<1..288)
                     /locus_tag="PLQ54_19405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95488.1"
                     /translation="MGRLLFFDANCTLGPRTAPRPETMLTDAEIAAELARHGITHALC
                     SHAHAQELDPATGNRRLASLDTAGWPVVVRRCLVVMPHHTGELPRAGRLRDY"
     gene            569..1396
                     /locus_tag="PLQ54_19410"
     CDS             569..1396
                     /locus_tag="PLQ54_19410"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOS95489.1"
                     /translation="MTIENAVALGVLQGLTEFLPVSSSGHLVVMPHLMPRLFGGASPP
                     LAFDVLVHVGTLVAAVAFMRTDVWQVIRGAWRLGKAPGRWRVLWRDDPWVRLVALIAL
                     STLVTAVIGLAGGEVFEPLFEAPRVACVGLLVTAALLFASHGLRREGNAVVEAHNMPV
                     VTALLVGLAQGVAITPGISRSGSTMCSGLLAGVSRDSAFRYAFLLMIPAVALAALKHV
                     TEGGIGGIDAAMALGAIASAVVGFAALAALRVLVVRGHLWVFAVYCVALALYGLARI"
BASE COUNT          188 a          468 c          559 g          320 t
ORIGIN      
        1 atagtcacgc agtcgcccgg cgcgaggcag ctcgcccgta tggtggggca tgacgacgag
       61 gcagcggcgc accaccacgg gccatcccgc cgtgtccagc gacgccagcc gtcggtttcc
      121 cgtggccggg tcgagctcct gggcgtgggc gtgggagcac agggcgtggg tgataccgtg
      181 ccgcgccagt tcagcggcga tctccgcgtc cgtcagcatc gtctcggggc gcggagcggt
      241 gcgtggtccc agagtgcagt tcgcgtcgaa gaacagcagc cttcccatct ccccacggta
      301 catccttgcc ggctgagcct gccacacacg ggtattccat gcggtcgccg cggggcctgc
      361 gcgtcccacg tcgtcggcgg acacgcgctt ggctttccgg gcgggtttgt gctactacta
      421 ggccgctgtt gggcgcgacc ctgccgccgg atcggcgagg gacgcgccgc tctgcggttg
      481 ggttgccagc cactggactc ggggtccctc gtaccgtctc tcactcgggg atgagctacg
      541 agcgtgccct tggtgtgaga gtgccctcat gacgatcgag aacgccgtgg ccttgggcgt
      601 gcttcaggga ctgacggagt tcctgccggt ctcgagctcc ggtcatcttg tggtgatgcc
      661 gcacctgatg cccaggctct ttggcggcgc ctcgccgccg ctggcattcg atgtgctggt
      721 ccatgttggg actctggtcg cggctgtggc gttcatgcgg acggatgtgt ggcaggtcat
      781 ccggggggct tggcggttgg gaaaagcgcc ggggcggtgg cgagtgttat ggcgcgatga
      841 cccgtgggtc cgtctcgtcg ccctgatcgc gctctcgact ctcgtgactg ctgtgattgg
      901 cctggccggt ggcgaagtgt tcgagccctt gttcgaggcg ccccgggtcg catgtgtggg
      961 tctcctggtg acggcggctc tcctgtttgc ctcccacggg ctgcgtcggg agggaaatgc
     1021 cgttgttgag gcgcataaca tgccagtagt caccgcactt ctggtgggcc tggctcaggg
     1081 agtggccatc acgcctggta tctcgagatc gggctcaacc atgtgcagtg gcttgctggc
     1141 cggcgtctca cgagactccg ctttccgcta tgctttcctg ctgatgatcc cggccgtcgc
     1201 tctggcggcc ctgaagcacg taacggaggg gggcataggc ggcattgacg cggccatggc
     1261 cctcggtgcc atcgcatccg cagtggttgg cttcgcggcg ttggctgccc ttcgagtgct
     1321 ggtcgtccga ggacacctct gggtcttcgc ggtgtactgc gtggctctag cgctctatgg
     1381 gctggcaaga atctaggttc gttcccggct cagagagccg ggtgggtgat catggagggg
     1441 ggggaggggt atccgccatt ggcggtaaca tagggccctt ggccgggcgg ctgggtctcg
     1501 cgaaggtcgc gcggaacgcc cgcctggtct ttcgg
//
LOCUS       DAOSPL010001325         1532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_82184_length_1532_cov_2.557211, whole genome shotgun sequence.
ACCESSION   DAOSPL010001325 DAOSPL010000000
VERSION     DAOSPL010001325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82184_length_1532_cov_2.557211"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1308)
                     /locus_tag="PLQ54_19415"
     CDS             complement(124..1308)
                     /locus_tag="PLQ54_19415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95490.1"
                     /translation="MRPEWLFCSLFATPLVLTAAAGAPGIEPLSELDRLNWCIGTQTF
                     GAAYRFTRETRLVETAQAILDMGSNVLKFGLEKNYFGPGRNVDDPDPRVHSLAELAAY
                     EPSHRRVLDMPFAYYIIWAYGFTPGWWSEGFSVADQKLEYDEMRALAAHLLTTYSGSG
                     KTFYLGHWEGDWHLRPSLNPADAVKPEAIEGMIDWLNVRQRAIDDAKRDVPHQDVEIY
                     GYCEVNLVRIAMEGRQSVTTDVVPRTTMDYVSYSSYDSGLDLIPALDYIESKLPPKPG
                     IPGKRVWIGEYGFPAFAHSPSEQDRLSRQVMRAGLAWGCPFVLYWEVYNNEVGKDGAQ
                     RGFWLIDDKGNKQPVYETHHAYYQWARHWATDFRAREGRLPTFDEFREAAVAYLDALP
                     GA"
BASE COUNT          245 a          493 c          536 g          258 t
ORIGIN      
        1 gctgttcgcc gtgctcgccg tgattgtgac cgaccgtggg gcactggacg tggcggcgct
       61 gctgaagcgg ctgtcgcaga acgaccaggg gtagaagcgg gcggctgccg gccacgccgc
      121 gggtcaggcc cccggcagcg catccaggta cgcgaccgcg gcctcgcgga actcgtcgaa
      181 ggtggggagc ctgccctcgc gggcgcggaa gtcggtggcc cagtggcggg cccactggta
      241 gtaggcgtgg tgtgtctcgt agaccggctg cttgttgccc ttgtcgtcga tgagccagaa
      301 gccgcgctgt gcgccgtcct tgccgacctc gttgttgtac acctcccagt agagaacgaa
      361 ggggcatccc cacgccagcc cggcacgcat cacctgccgc gagagtcgat cctgctcgga
      421 cggcgagtgg gcaaaggccg ggaatccgta ctcgccgatc cacacgcgct tgccgggaat
      481 gcccggcttg gggggcagct tggactcgat gtagtcgagg gccgggatca ggtcgaggcc
      541 gctgtcgtag ctcgaatagg agacgtagtc catcgtcgtc cgaggcacga catcggtggt
      601 gaccgactgg cgaccttcca tggcgatgcg gacgaggttc acctcgcaat agccgtagat
      661 ctcgacgtcc tggtgcggca catcccgttt ggcgtcatcg atggcccttt gccgcacgtt
      721 tagccagtcg atcatgccct cgatggcctc gggcttcact gcgtccgccg ggttcaggct
      781 cggacggagg tgccaatcgc cctcccagtg acccaggtag aacgtcttcc cggagccgct
      841 ataggttgtc agcagatgcg ccgccagggc cctcatctca tcgtactcga gcttctggtc
      901 cgccacggag aagccctcac tccaccaacc cggggtgaag ccataggccc agatgatgta
      961 gtaagcaaaa ggcatgtcga gcacgcggcg gtgggacggc tcgtaggccg cgagctcggc
     1021 cagggagtgg acgcgcggat cggggtcatc cacgttgcgt cccgggccga agtagttctt
     1081 ctcaagcccg aacttcagga cgttcgaacc catgtcgaga atcgcctgag ccgtctcgac
     1141 gagacgggtc tcccgggtga accggtacgc cgcgccgaat gtctgagtgc cgatgcacca
     1201 gttgagccga tcgagctcgg aaagcggctc gatgcccggg gctccggccg cagccgtgag
     1261 caccaggggc gtggcgaaga gcgaacagaa gagccattcg gggcgcatgg ccgtctcctc
     1321 gttcgggagt gggactgcgt cggcgtggcg agcggacctc cggagccggt cgcggtgctc
     1381 gtgtcagccc tccgcatgtc gcgggacggc attcgccacg ccgtggcggc tccggggtcc
     1441 aatgtaccat cttggcctgc ccgcgtcccc ccttggcctc gaccggccac gaacggacga
     1501 ggtggagggc ggcgcggagc ggctcgccgg cc
//
LOCUS       DAOSPL010001326         1530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_82329_length_1530_cov_3.012881, whole genome shotgun sequence.
ACCESSION   DAOSPL010001326 DAOSPL010000000
VERSION     DAOSPL010001326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82329_length_1530_cov_3.012881"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..>1530
                     /locus_tag="PLQ54_19420"
     CDS             33..>1530
                     /locus_tag="PLQ54_19420"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOS95491.1"
                     /translation="MSGPLPRHEDTALLLHFDEGQGRPRDSSGHGRTMDALPDPFEEK
                     GTLSATYDFLERDCGVRWYVPGELGTAYPSTRTLAVERRDRRRSPAMIHRWITPTALF
                     VPTPQEPVPANEVALWKLRMRIGGQPFWVCHSFEGYYDRFLKDHPDWFAQGYEGRPPQ
                     MCFTNPEFIAQVVQDARDYFDGKGAQPGAAAMGDVFGLVPMDNMSWCKCPRCQAELNE
                     RERENPQFSNGKASDYIWNFVNKVAREVGKTHPDKWIGALAYSDYAYYPTRVVLEPNV
                     LVQMCLHTRNWWVPSMERNDMRVFSQWVERSGGRRPLYLWLYYCFPALNAQYENYNGF
                     PSYFAHTAVRQMRMYHEAGIRGIFIEHSSEFAQTHLMDVPDLYVTLRLADDPSLDGNR
                     LIDEFFTRFYGAAGEPMRALYEAIERAYSSPASYPEEVQKTPAHTHQNEKLAWGSVGN
                     PERMRAFAALMERAKAAATTDIEKRRVALFERGIWEYMLAGAKQYQEKL"
BASE COUNT          277 a          488 c          527 g          238 t
ORIGIN      
        1 tctcgaccac gccccggacc gagggcgcgt tcgtgtccgg gccgctcccg cgccacgagg
       61 acacggcgct gctactgcac ttcgacgagg gtcagggccg gccgagggac agcagcggcc
      121 acgggcggac catggatgcg ctgcccgacc cgttcgaaga gaagggcacg ctgagcgcga
      181 cctacgactt tctcgaacgc gactgcggcg tccggtggta cgtgccgggc gagctgggga
      241 cagcctatcc gtcaacgcgg acgcttgccg ttgagcgcag ggaccggcgg cggtcgccgg
      301 ccatgatcca ccggtggatt acgcccacgg cgttgttcgt gccgacgccg caggagccag
      361 tgccggcgaa cgaggtcgcc ctgtggaagc tgcggatgcg gatcggtggc cagccgttct
      421 gggtctgcca ctcgttcgag gggtactacg accggttcct gaaggaccac ccggactggt
      481 tcgcccaggg ctatgagggc cgcccgcccc agatgtgctt cacgaacccc gagttcatcg
      541 cccaagtggt ccaggatgcg cgagactact tcgacggcaa gggggcccaa ccgggcgccg
      601 cggccatggg tgatgtgttc ggcctggtac cgatggataa catgagctgg tgcaagtgcc
      661 cgcgctgcca ggcggagctg aatgagcgag agcgggaaaa cccgcagttc tccaacggca
      721 aggccagcga ctacatctgg aacttcgtca acaaggtggc ccgcgaggtc gggaagacgc
      781 acccggacaa gtggatcggc gcgttggcct actcggacta tgcgtactac cccacccgcg
      841 tggtgctcga gccgaacgtc ctggttcaga tgtgcctgca cacgcgcaac tggtgggttc
      901 cgtcgatgga gcgcaacgac atgcgcgtgt tcagccagtg ggtggagcgc agtggaggcc
      961 ggcggccgct ctatctctgg ctctactact gcttcccggc tctgaacgcc cagtacgaga
     1021 actacaacgg gttcccgagc tacttcgccc acaccgcggt gcgccagatg cgcatgtacc
     1081 acgaggcggg cattcggggc atcttcatcg agcactcgag cgagttcgcc caaacgcacc
     1141 tgatggatgt gcccgacctc tacgtgaccc tgcgactggc cgacgacccg tcgctggacg
     1201 gcaatcggct gatcgacgag ttcttcacgc gcttctacgg tgcggcgggt gagccgatgc
     1261 gcgcgctcta cgaggccatc gagagggcct actcgagccc ggcgagctat ccggaggaag
     1321 tccagaagac gcccgcacac acacaccaga atgagaagct cgcatggggt tcggtcggca
     1381 acccggagcg catgcgcgcg ttcgccgcgc tgatggagcg tgccaaggcg gccgcgacga
     1441 ctgacatcga gaagcgccgg gtggcgctgt tcgagcgcgg catctgggag tacatgctgg
     1501 ccggagcgaa gcagtaccag gagaagctgg
//
LOCUS       DAOSPL010001327         1529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_82397_length_1529_cov_3.739484, whole genome shotgun sequence.
ACCESSION   DAOSPL010001327 DAOSPL010000000
VERSION     DAOSPL010001327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82397_length_1529_cov_3.739484"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..574
                     /locus_tag="PLQ54_19425"
     CDS             137..574
                     /locus_tag="PLQ54_19425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95492.1"
                     /translation="MDDQPPTEINPYIYRGDNYGGIMAPVFAVVFGAVCVFVLATSLR
                     GDGPPLWFALLFISLVMAIVGTTTFLFATKIVVSHTLISWNYLGRRGGSALVENIESV
                     GAFGWAMPGWVQLKDGRRIWIQTTPGFRHLREAIGQVSPGARV"
     gene            complement(637..996)
                     /locus_tag="PLQ54_19430"
     CDS             complement(637..996)
                     /locus_tag="PLQ54_19430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95493.1"
                     /translation="MMIRMELALPDDIDPELWFPCGDVAGARDYLYSSVWHTAPGRMG
                     AYCAAKDVYFRASAEEVPADAPIATRYWVRGYLAGSLPSPPYDDPSEEELGAWRARAH
                     AYFASGSWPSAPEEVPD"
     gene            complement(1061..>1529)
                     /locus_tag="PLQ54_19435"
     CDS             complement(1061..>1529)
                     /locus_tag="PLQ54_19435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95494.1"
                     /translation="LVNEMYRRHGAYLKALLKSDDHSDVVAHENTIKLHTHITKRHSQ
                     IGTLRTFADETETWEDTRAIEQAARAAFIKDPNVDQLVLEARLEWMAGSGAAHGLVWP
                     LFGTAGTRVLGPADKQGRAVIEAGGSYARVLNAYLLAYWMTAAGWKLLRRRGL"
BASE COUNT          254 a          503 c          478 g          294 t
ORIGIN      
        1 gaagttcgta ttgccgcctg ccgctaccac tacgggtagc gcgatctgcg gcatgtccgg
       61 taaaccaccc acgctgatgc tcgcccgtcc gagggtggcg acagactcgc cctgcctttg
      121 accgatcatg gacctcatgg acgatcagcc tccaaccgag atcaacccct acatctaccg
      181 aggggacaac tacggcggga tcatggcccc cgtcttcgcc gtcgtgttcg gggctgtgtg
      241 cgtcttcgtg cttgctacat cccttcgtgg ggatgggccg cccctttggt tcgccctcct
      301 gtttatctcg ctcgtgatgg ccatcgtggg caccaccaca ttcctcttcg ccacgaagat
      361 cgtcgttagc cacacgctca tcagctggaa ctacctgggc cggcgaggtg ggtctgccct
      421 cgtggagaac atcgagagcg tcggagcctt cgggtgggcc atgccggggt gggtgcaact
      481 caaggacggc cgccggatct ggattcagac cactcctggc tttcgccacc tgcgcgaggc
      541 gatcggccag gtcagtcccg gcgcccgcgt ctgaactcca cctgccgctt gaggagcagg
      601 cacaagtgcg tctggcacgc ggcgtgtgcg gatgcgtcag tcgggtacct cctcaggcgc
      661 tgacggccat gatcctgagg cgaagtacgc gtgggcgcgg gcacgccatg cgcccagctc
      721 ttcctcgctc gggtcgtcgt agggagggct aggtaggctg cccgccaggt acccacgaac
      781 ccagtacctc gtagcgatcg gggcatcggc tgggacctcc tcagcggagg ctcggaagta
      841 cacgtccttg gcggcgcagt acgcgcccat ccgacctggg gcggtatgcc agacagatga
      901 atacaggtag tcgcgtgcgc cggctacatc gccacaggga aaccacagct ccgggtcgat
      961 gtcgtcaggc agggcaagct ccatgcgaat catcatgcct gcgagtgacc ctgtctgcgg
     1021 cagatccggg ccctcgaggc agatactcaa ggcggccggc ctagagacca cgacggcgca
     1081 gcagcttcca accagcggcg gtcatccagt acgccaagag gtaagcgttg agcacacggg
     1141 catacgaccc accggcctcg atgaccgcgc ggccctgttt gtctgccggg cccaggacgc
     1201 gcgttccagc cgtcccgaac agtggccata cgagtccatg tgcggcaccg gaaccggcca
     1261 tccactccaa cctcgcctcg aggacgagct ggtccacgtt cgggtccttg atgaacgccg
     1321 cgcgcgctgc ctgttcgatc gcccgcgtgt cttcccaagt ttcggtctcg tccgcgaacg
     1381 tgcgcaacgt gccgatctgg ctgtgccgct tcgtgatgtg cgtgtgaagt ttgatggtgt
     1441 tctcgtgcgc caccacgtcg ctgtgatcgt ctgacttgag tagcgctttc aagtaggcgc
     1501 cgtgtcggcg gtacatctca tttactagg
//
LOCUS       DAOSPL010001328         1528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_82484_length_1528_cov_3.613714, whole genome shotgun sequence.
ACCESSION   DAOSPL010001328 DAOSPL010000000
VERSION     DAOSPL010001328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82484_length_1528_cov_3.613714"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PLQ54_19440"
     CDS             <1..417
                     /locus_tag="PLQ54_19440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95495.1"
                     /translation="GNLLSPYLYSGGNCRVCFDWASRELTAASAFDPLRPAFFAGGAA
                     LTGGLILARRAWVRFPLLPLGYAMVPSWATLVLWFPCLVAWLVKKTLFRLYGYRGYLA
                     ARPLFVGMIVGEVVVALVWTAIAVAFRLQPPSMPLG"
     gene            1025..>1528
                     /locus_tag="PLQ54_19445"
     CDS             1025..>1528
                     /locus_tag="PLQ54_19445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95496.1"
                     /translation="MTRVAPPRYTQNVSDTGSAQEDLRSLECFHQVYFRTSKGGLGVE
                     YDNARAEFAAVFAPSEVRILRDRPSRCRLSVELRITEEALAARAVRLGYTLGIVRVTR
                     HPVEGRAPGGTPQMRGRWPIGWLREGNSELLFEPVFVASEAQRMAESPHVFAFPQTRN
                     GRVLAASR"
BASE COUNT          215 a          500 c          543 g          270 t
ORIGIN      
        1 gggaacctgc tcagcccgta cctctactcc ggcggcaact gccgcgtgtg tttcgactgg
       61 gcgagccggg agctcacggc ggcctcggca ttcgatccgc tgcggccggc cttctttgcg
      121 ggcggcgcgg cgctgacggg aggcctgatc ctcgcccgcc gtgcctgggt gcgcttcccg
      181 ctgctgccgc tgggctatgc catggtgccc tcatgggcga cgctcgtgct gtggttcccc
      241 tgcctggtgg cctggctggt gaagaagacc ctctttcgcc tctacggcta ccgaggctac
      301 ctggcggccc ggccactctt cgtgggcatg atcgtaggcg aggtcgtcgt ggcactggtc
      361 tggacggcca tcgcggtcgc gtttcgcctg cagccgccct cgatgccgct gggctgagag
      421 agggcactgc acgtggctca ggaagaccgg tcaggagaac actgcccacg atcatggtct
      481 gctgccccgc atgggatcct gatagggacg catagggggg gtggtgtcgg gctggcgtgt
      541 gagccgcttc cggtccaggc tacgtgtatg ccacggctac ctggagatct ccggcccagt
      601 tacgcgctgg cagacggatc cagcgctctg acgtcggtct ctgggttcct gcatggtggt
      661 ctaccagccc cagggcgatc gtgcgatcgg cgcggagggg ctggagatgc cgccaggcgt
      721 ggtacggagc caagacaaca cacgtcggga gctcgagtcg tcgggcggaa gagcggtctc
      781 gcgcaaggag gcgaaagcgc cgatggcgag cccgggaccg ggccatgcgg ggcatggcag
      841 actcccgctt tgggagaccc gaagggtccc gtaccgttcg gttcatcgcc tgtcttggtc
      901 tccgtcgcgc actcaggccg agcagtcgcc cgccgaacag ggccaatcgc atcgcgtaga
      961 tctaccttac tacgtccgac cgcggcccgt tcgggtcatg acctggtcct gggggcaggg
     1021 gggcttgacg cgggtcgcgc cgccgcgcta cacccagaac gtgtccgaca ctggcagtgc
     1081 gcaagaggat ctccgtagcc tggaatgctt ccatcaggtc tacttccgca ccagcaaggg
     1141 cgggcttggt gtggagtacg acaatgctcg ggccgagttc gccgcggtgt tcgcacccag
     1201 tgaggtgcgc atcctccggg accgcccctc gcgctgccgc ctcagcgtcg aactccggat
     1261 caccgaagag gccctggccg ctcgggccgt gcggctcggc tacacactgg gcatcgtccg
     1321 cgttacgcga cacccggtgg agggacgcgc cccgggtggt acgccccaga tgcggggccg
     1381 ctggcccatc gggtggctgc gcgagggcaa ctcggagctt ctcttcgagc cggtgttcgt
     1441 cgcgtccgag gcgcagcgga tggccgagtc gccacacgtg ttcgctttcc cgcagacgag
     1501 gaatgggcga gtcctcgcgg catcgaga
//
LOCUS       DAOSPL010001329         1528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_82512_length_1528_cov_2.431772, whole genome shotgun sequence.
ACCESSION   DAOSPL010001329 DAOSPL010000000
VERSION     DAOSPL010001329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82512_length_1528_cov_2.431772"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..>1528
                     /locus_tag="PLQ54_19450"
     CDS             29..>1528
                     /locus_tag="PLQ54_19450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOS95497.1"
                     /translation="MRDLASGTMVAAARVPAAPGGMVEATLPLGAAMRRWDEFSPSRY
                     ELVATLRLPDGASDERRVRFGMRDLEVRGGRLVLNGRPAFLRGTLECCVFPLTGYPPT
                     DVESWLRIFRIARSYGLNHMRFHSWCPPEAAFEAADRTGFLLHVEAPQWTFDVGQDPP
                     RDEFIRQEVMRILDAYGNHPSFGMLCMGNELTGDVSFLQTLVLFGREHDPRHLYTSST
                     AWSFGPNDQYNVAAVRGLHGPTTDADFAEAVRQSPVPLISHEVGQWSVFPNIREIRKY
                     TGVLRPRNFERVRDSLRAHHLLSQADDFTWVTGRLSAQLYKEEIEVLLRTPGLGGFQL
                     LDLHDFPGQGTALVGMLDAFWDSKGFISASEFSRFCGPTVPLLRMKQRTYTTADTLSA
                     RVQISHFGPRRLRGVVPQWSLVAEDGRRIHSGALPARDIPTGQLSDLGAIEVALKGVR
                     APVRATIEVRLSGLGIANDWNIWVYPENEAAGEPPDVLVTPSVTEALAAL"
BASE COUNT          238 a          500 c          524 g          266 t
ORIGIN      
        1 cgaagccagt ggagatctgc agctcgccgt gcgcgatctc gcctccggga ccatggtggc
       61 ggcggcccgc gtccccgccg cgccgggcgg gatggtcgag gccacactgc cgctgggtgc
      121 cgcaatgcgc cggtgggacg agttctcgcc gagccgttac gagcttgtcg ccacgctccg
      181 cctccccgat ggtgcctccg acgagcgtcg tgtgcgcttc ggcatgaggg acctggaggt
      241 gcgcggtggt agactggtgc tcaacgggcg gccggcgttc ctgcgaggta cgctggagtg
      301 ctgcgtcttc ccgctcaccg gctatccgcc tacagatgtc gagagctggt tgcgcatctt
      361 ccgcattgcc cggtcgtacg ggctgaacca catgcgcttc cactcgtggt gtccgcctga
      421 agcggccttc gaggcagccg accggaccgg gttcctcttg cacgtcgagg caccgcaatg
      481 gaccttcgac gtgggtcaag acccaccaag ggacgagttc atccgccagg aagtgatgcg
      541 gatcctcgat gcgtacggca accacccttc gttcggcatg ctgtgcatgg ggaacgagct
      601 tacaggcgac gtgagcttcc tgcagacgct ggtgctgttc gggcgcgagc acgacccgcg
      661 gcacctgtac acatcatcca cggcatggtc gttcggcccg aacgaccagt acaacgtggc
      721 cgccgtgcgc gggcttcatg gcccgacgac cgacgccgac ttcgcagagg ccgttcggca
      781 gagccccgtg ccgctcatct cccacgaggt gggccagtgg agcgttttcc cgaacatcag
      841 ggagatccgg aagtacaccg gagtgctcag gccccggaac ttcgaacggg tccgtgatag
      901 cctgagggcg catcacctcc tgagtcaggc cgacgacttc acttgggtca cgggccggct
      961 gtccgcgcag ctctacaagg aggagatcga ggtgctgctg cggacgcccg gtctgggtgg
     1021 gttccagttg ctggacctgc atgatttccc gggccaggga acggcgctag tgggcatgct
     1081 cgatgccttc tgggacagta agggcttcat cagcgcctcc gagttcagcc ggttctgtgg
     1141 gcccacggtt ccgctgctgc ggatgaagca gcgcacctac acgacggcgg acaccctttc
     1201 ggcacgcgtg cagatctctc actttgggcc gcgacgtctg cgaggcgtgg tgccccagtg
     1261 gtcgctggtc gccgaggacg gccgccggat ccactcaggt gcactgcccg cccgcgacat
     1321 cccgactggg cagctcagcg acctcggcgc catcgaggtg gctctgaagg gggtgagggc
     1381 gccggtaagg gccacgatcg aggtgaggct gagcgggcta ggcatcgcca atgactggaa
     1441 catctgggtg taccccgaga acgaggccgc cggcgagccg ccggatgtgc tggtcacccc
     1501 ctcggtgacc gaggccctgg ccgctctc
//
LOCUS       DAOSPL010001330         1525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_82716_length_1525_cov_3.394558, whole genome shotgun sequence.
ACCESSION   DAOSPL010001330 DAOSPL010000000
VERSION     DAOSPL010001330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82716_length_1525_cov_3.394558"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="PLQ54_19455"
     CDS             <1..965
                     /locus_tag="PLQ54_19455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95498.1"
                     /translation="ADKRIMYWEVFNEPAFLAASDATAATQAAKDVALALSSIDAGAR
                     LGGLGLGVAEASQMAAIVKAGEGLPFYSWHLYGSRGLTSSPEQLMAAARLARQDGAPG
                     VLDPGQVDKLLTSLPNWKEKLLFVTECAANGLRDEVTGAARDPRLVTRLHAAWFASAM
                     ISFAPFVDAVFLSDAVGASWGVLDAEGSPYPAYWAAYLISRHMPRGATICEIDITPNQ
                     PLRALCVSGKQKAVLLVNQANAEARLSLRASGVASQSDAKLLVVGDKAGMELAESAVS
                     PQYSAMAKGDGTQPAGGKLYAAKLDGLSLGPFGVAIVLFDAPAA"
     gene            complement(971..>1525)
                     /locus_tag="PLQ54_19460"
     CDS             complement(971..>1525)
                     /locus_tag="PLQ54_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95499.1"
                     /translation="WLPRRLHSGNSEHGYMALGVGSEVVDVEQVATVPRALRIPCRAV
                     VRVDSTGGSFVSLVELRSRRGEILYTTRHQAGAGSAVEAALWGAYHAHSAAHTLGVRS
                     LDVYLSEPDAVDILARRTQAPEGASKAFFWARTSARKLSRVNYHEISDAIRDEMRQLV
                     LTREPVLPPTSTRTLSLFGGETAA"
BASE COUNT          242 a          508 c          501 g          274 t
ORIGIN      
        1 atgcggacaa gcggatcatg tactgggagg tcttcaacga gcccgctttc ctggcggcgt
       61 cggatgccac cgcagccacc caggcggcca aagacgtggc actggccctc tcgtcgatcg
      121 atgcgggcgc gcgactgggc ggactggggc tcggcgtcgc cgaggcgtca cagatggcgg
      181 ccatcgtcaa ggccggagaa gggctgccgt tctactcgtg gcatctctac ggaagtcgag
      241 gccttacgtc gagcccggag caactgatgg ccgccgcgcg cttggcccgc caggatggtg
      301 cgccgggtgt gctcgatccc ggtcaggtcg acaaactgct cacatcgttg ccaaactgga
      361 aggagaaact gctcttcgtc acagagtgcg cggcaaacgg cctccgggat gaagtcaccg
      421 gcgcggcgcg cgatccgcgg ctcgtgaccc gtctccatgc tgcatggttc gcctccgcca
      481 tgatctcgtt tgcgcccttt gtggacgcgg tgttcctctc ggatgccgtg ggtgcgagct
      541 ggggggtgct ggacgcagag gggtccccct accccgccta ctgggccgcc tacctgatct
      601 cgcgtcacat gccacgtggc gcaacgatct gcgagatcga catcaccccg aaccagccgc
      661 ttcgggccct ctgtgtcagt gggaagcaga aggccgtctt gctcgtcaac caggccaacg
      721 ccgaggcgcg cctgtcgctt cgggcgtctg gggtcgcatc gcaatccgac gcgaagctcc
      781 tggtggtggg tgataaggcg ggtatggagt tggccgagtc tgccgtgagt ccccagtact
      841 ccgccatggc caaaggcgat gggacacaac cggccggcgg gaagctctac gcggccaaac
      901 tcgacggcct ctcgctcggt ccgttcggcg tcgccatcgt cctgttcgac gcgccggccg
      961 cctgatcgcg ttaggccgcg gtttcccctc cgaataagct cagcgtccgc gtcgatgtgg
     1021 ggggcagcac gggttcgcgc gtgagtacca gctgtctcat ttcgtcacgt atcgcgtccg
     1081 agatctcgtg gtagttcacc cggctgagtt tgcgggcact ggtgcgtgcc cagaagaacg
     1141 ccttcgacgc gccctcagga gcttgagttc gacgcgccag gatgtccaca gcgtcgggct
     1201 cactcaggta gacatcgagg ctccgcacgc ccagtgtgtg ggcggctgag tgggcgtgat
     1261 acgcccccca cagtgcggcc tccaccgcgc taccagcgcc agcctgatgc cgcgtggtgt
     1321 agaggatctc cccacggcgg ctgcggagct ccacgagact gacgaagctg ccccccgtgc
     1381 tgtccaccct cacgacggcg cggcacggga tccgtagtgc acgaggtacc gtcgctacct
     1441 gttccacgtc gaccacctcg ctcccaactc caagagccat gtacccgtgc tcggagttcc
     1501 ctgagtgcag tcgacgtggg agcca
//
LOCUS       DAOSPL010001331         1522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_82965_length_1522_cov_3.054533, whole genome shotgun sequence.
ACCESSION   DAOSPL010001331 DAOSPL010000000
VERSION     DAOSPL010001331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82965_length_1522_cov_3.054533"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..605
                     /locus_tag="PLQ54_19465"
     CDS             30..605
                     /locus_tag="PLQ54_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95500.1"
                     /translation="MRAALMPLTLTVALLALACPACRRPPEAPESAIEVDPNIRDARK
                     SLAPPDGGLPPFGGMKGPSIATPEGKAAFSAGGGPGKGLAPKGEIDFGVSVPESTLKR
                     LDLPVYAAAVPEAHTNAETSVTATYKAKDPYDDVKAWYTKELPGWKQTSMEMGGGSAC
                     MFASADGKATIAISKVQDTGETLIVLTRQAK"
     gene            complement(625..>947)
                     /locus_tag="PLQ54_19470"
     CDS             complement(625..>947)
                     /locus_tag="PLQ54_19470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95501.1"
                     /translation="DLNCAPVLRGKQRLVVCDALLCCYDGGPSHKPETTEVRNSLLFS
                     EDAVAVDWLCARMIDELRAARQLPSLKEEGREPRFIAEAAAPSRGIGVAGESAVEVVD
                     VPLG"
     assembly_gap    948..957
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<958..1508)
                     /locus_tag="PLQ54_19475"
     CDS             complement(<958..1508)
                     /locus_tag="PLQ54_19475"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOS95502.1"
                     /translation="MTHPDAFRGDTVCTDAVEQMVDAGMRRLLGTATAAEAFRRLFQP
                     GDVVGLKVNCLAGPLLSTHPEVTYCVARGIVAVGIPSRNVIVYDRESSELASAGYDVT
                     VRPADVRCIGSDMAGYDSTPTVQGTVGTCFSRIVSERVSALVNLPVLKDHDLAGLSGA
                     LKNHYGSIHNPNKLHMNHCSPYVA"
BASE COUNT          299 a          519 c          474 g          220 t
ORIGIN      
        1 gcagcgggtg taggatgagg agatcgtgaa tgagagccgc actaatgcca cttactctca
       61 ctgtggcgct gcttgcgctg gcgtgcccgg cctgccgcag gccgccggag gcgcctgaaa
      121 gcgccatcga agtcgaccca aacatccggg acgcaaggaa gagtctcgcg ccccccgacg
      181 gagggctacc cccgttcggc gggatgaaag gccccagcat cgccaccccc gaaggcaagg
      241 ccgcattctc cgccggaggc ggccctggca agggactcgc gccaaagggc gagatcgact
      301 ttggcgtcag tgtgcccgag agcaccctca agagactcga ccttcccgtc tacgccgcgg
      361 cggtccccga agcccacacg aacgccgaga ccagcgtgac cgcgacctac aaggccaagg
      421 atccctacga cgacgtcaag gcctggtaca ccaaagagct cccggggtgg aagcagacga
      481 gcatggagat gggtgggggc agtgcctgca tgttcgccag tgccgatggc aaggcaacga
      541 tcgcgatcag caaagtccag gacacgggag agacgctcat cgtgctgacc cgccaggcca
      601 agtagcccgg ggcccggcca cgtactatcc cagagggaca tccaccacct cgaccgccga
      661 ctcgcccgcc acgccgattc ccctcgacgg agccgctgcc tcggcaatga agcgcggttc
      721 gcgcccttcc tccttgaggc tcggcagttg cctggccgct cggagctcgt cgatcatccg
      781 cgcgcaaagc cagtcgacgg cgactgcgtc ctccgaaaag agcaagctgt ttcggacctc
      841 agtcgtctcc ggcttgtgcg atggacctcc gtcgtagcag cacagcaggg cgtcgcaaac
      901 gaccaaccgc tgtttgcccc gcagcaccgg cgcgcagttg aggtcggnnn nnnnnnngcc
      961 acatagggcg aacagtggtt catgtgcagt ttgttggggt tgtggatcga cccatagtgg
     1021 ttcttcagag cccccgatag cccggccaga tcgtgatcct tgagaacagg caggttcacc
     1081 agagcgctga cgcgctccga gacgatacgc gagaagcaag tcccgacagt cccctgcacc
     1141 gtcggggtcg agtcataccc ggccatgtcg gacccgatgc agcgcacgtc agctggccgc
     1201 acggtcacgt catagccggc cgaggcaagc tccgacgact cccggtcgta cacgatcacg
     1261 ttgcgcgacg ggatccccac cgcgacgatg ccgcgcgcca cgcagtaggt aacctcgggg
     1321 tgcgttgaca gcagtggacc tgccagacag ttcaccttga gcccgacaac gtcgcccggc
     1381 tggaagagcc gacggaatgc ctcagcggcg gtcgcggtgc ccagcagcct ccgcatgccc
     1441 gcatcgacca tctgctccac cgcatcggtg cagacggtat ccccacggaa cgcatctgga
     1501 tgcgtcacgc ggacgacacg cg
//
LOCUS       DAOSPL010001332         1522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_82968_length_1522_cov_2.918882, whole genome shotgun sequence.
ACCESSION   DAOSPL010001332 DAOSPL010000000
VERSION     DAOSPL010001332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82968_length_1522_cov_2.918882"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1522)
                     /locus_tag="PLQ54_19480"
     CDS             complement(<1..>1522)
                     /locus_tag="PLQ54_19480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95503.1"
                     /translation="ALTVAGYFAEPGNGLAELRSHVPAWAMPQGTETIRGMYEGIEGA
                     SVPWSDWAGPLTFWSLFMLVIFLTTLCAAWAMRPQWTHREKLTYPLVDVVLRVTSSAS
                     GAGRPTLLRDPVMWIGFGVAALHNVLNILHAFNPQTPCLGSVLELGALFTERPMSGLA
                     PLIFYHRPELIGLGYLVPVDVGLTVWVSHLLLRLTRVAALMGGNDNPALPYPDEQSVG
                     AYLRRLFRSAICGGWGAHRISVLGVIGGFAGTVAMAMALGMKLGWALLFVSMVYAIAF
                     VHARIRAETGTPVMSVFPWKRHDVFVASLGGRLLGRRAPANLAALSMLGWLGRGHFPE
                     LMSFQLEDLEMAERAGIRLRRMASGLLVALLFGMIAAYWWHLKAYYGYGANVLEGGTT
                     EGGYRVHVQHEIFLGAARQLLSPTAPDPARCTAIGVGGVIVTVMHALRVWLVRFPLSP
                     LGYAMTAAYGYLLWGPFLVASVAKITTLKLGGARLYQRVEPAFLGLALGQFMTAGVL"
BASE COUNT          279 a          481 c          545 g          217 t
ORIGIN      
        1 gagcaccccg gcggtcatga actgccccag agcgaggccc aggaatgcgg gttcgacgcg
       61 ctggtatagg cgagctcccc ccagcttgag ggtggtgatc ttcgctacgg aagccacgag
      121 gaacgggccc cacagcaagt agccgtaggc ggccgtcatc gcgtagccga gggggctcag
      181 tgggaaccgg accagccaga cgcggagtgc gtgcatgacg gtcacgatca caccgcccac
      241 gccgatggct gtgcatcggg ccgggtccgg cgcggtgggt gataggagtt ggcgggcggc
      301 gcccaagaag atctcgtgct ggacgtgcac gcggtagccc ccctcggtgg tgccgccctc
      361 gaggacgttg gccccgtagc cgtagtaggc cttgaggtgc caccagtagg ctgcgatcat
      421 cccgaacagc agcgcgacca gcaggcccga tgccatccgc cgcagccgga tgcccgcgcg
      481 ctcggccatt tccaggtcct cgagctggaa ggacatgagt tcggggaagt ggcctcgacc
      541 cagccacccg agcatactca gtgctgcgag gttcgccggt gccctgcggc cgagcagcct
      601 gccccccagt gacgcgacga acacgtcgtg gcgcttccac gggaagacgg acatcacggg
      661 tgtcccggtt tcggcgcgga tccgtgcatg cacgaaggcg atggcgtaga ccatggagac
      721 gaacagcagg gcccatccga gcttcatccc cagagccatt gccatcgcga cggtgccggc
      781 gaacccgccg atgacgccga gaacggagat tcggtgggcc ccccaccctc cgcagatcgc
      841 ggatcgaaat agacggcgca ggtaggcgcc taccgactgt tcgtcaggat atgggagtgc
      901 ggggttgtcg ttgccgccca tcagggcggc cacgcgggtg agacgtagta gcaggtgcga
      961 gacccacacg gtgaggccca catcgaccgg taccaggtag ccgaggccga tgagttcggg
     1021 gcggtggtag aagatcaggg gcgccagccc ggacatgggg cgctcggtga agagggctcc
     1081 cagctcgagc acactgccca ggcatggtgt ctgcgggttg aaggcatgga ggatgttgag
     1141 gacgttgtgt agggccgcga cgccgaagcc gatccacatg accggatctc ggagcaaggt
     1201 gggccggccc gctccggatg ccgacgaggt gacccggaga accacgtcaa ctagtgggta
     1261 ggtcagcttc tcgcggtggg tccactgggg gcgcattgcc cacgccgcgc agagggtggt
     1321 caggaagatg acgagcatga acagcgacca gaaggtcagg ggaccggccc agtcgctcca
     1381 cgggactgag gcgccctcga tgccctcata catgccccgt atcgtctcgg tgccctgagg
     1441 catcgcccag gccggcacgt ggctgcgcag ctcggccagc ccattgccgg gctcggcgaa
     1501 gtagcccgcc accgtgaggg cg
//
LOCUS       DAOSPL010001333         1519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_83174_length_1519_cov_3.471311, whole genome shotgun sequence.
ACCESSION   DAOSPL010001333 DAOSPL010000000
VERSION     DAOSPL010001333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83174_length_1519_cov_3.471311"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="PLQ54_19485"
     CDS             <1..937
                     /locus_tag="PLQ54_19485"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95504.1"
                     /translation="GFPISRRPYDVLADELGHTASGWLSRVRRLSEEGIVRGVSAVFE
                     PTRIGYVTSLATLAVPPDRLEAVAAMVSEYPEVTHNYSRDHTYSLWFALVAHGQTRLD
                     SALRDIEARAACGPVHALPTLRRFKIRVDFDFGTGAREAPRLAGTDPAEPIDVDPALV
                     HRLCQPLPLVAEPYDALAASLGRDPDAVIAEVVRYTRAGAIRRLGALVRHHQAGFGAN
                     AMVVFRVPEEAMVAAGERLAEFAAVSHCYERPPLPRLPHNLYAMVHAHHDDECRALAA
                     QMARAIGVRDYLALFTVTEYKKASPVYFAEAAGNR"
     gene            complement(1006..>1519)
                     /locus_tag="PLQ54_19490"
     CDS             complement(1006..>1519)
                     /locus_tag="PLQ54_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOS95505.1"
                     /translation="PRDVETVAEHCDILQIGTRNMQNFPLLTEVGKSGRAVLLKRGMA
                     STIEDLLMAAEYVLSEGNERLLLCERGVRTFEPSTRYTLDLSAVAVIRRYSHLPILVD
                     PSHATGHWWAVPAMAKAALAAGADGLVIEVHPRPKEAYCDGAQSLKCETFETLMAELK
                     PIAAAVGRKL"
BASE COUNT          214 a          531 c          513 g          261 t
ORIGIN      
        1 cggctttccc atctcccggc gcccctacga cgtgctcgcc gacgagctcg ggcacaccgc
       61 aagcggctgg ctctcgcggg tgcgccggct ttccgaggag ggcatcgtcc gcggcgtctc
      121 agcggtcttc gagcccaccc gcatcgggta cgtgacctcg ctggccacgc tggccgtgcc
      181 gcctgaccgc ctcgaggccg tggccgcgat ggtgagtgag tatccggagg tcacccacaa
      241 ctactctcgc gaccacacgt actccctctg gttcgcgctg gtcgctcacg gccagacgcg
      301 cctggactcg gcactccggg acatcgaggc acgggccgcc tgcggcccgg tccatgctct
      361 ccccacgctg cgtcggttca agatccgcgt tgacttcgac ttcggcacgg gtgctcgcga
      421 ggctccgcgc ctcgccggaa cggaccctgc agagcctatc gacgtcgatc cggccctcgt
      481 gcaccggttg tgtcagccat tgcctctggt agcggagccc tacgacgcgc tcgcggctag
      541 cctgggccgc gatcccgatg ccgtcatcgc cgaggtcgtc cggtacaccc gggcgggcgc
      601 gatccggcgc ctcggtgccc tcgtacggca ccatcaggcg ggttttggcg ccaatgccat
      661 ggttgtgttt cgggtgcccg aggaggccat ggttgccgcc ggggagcgtc tcgccgagtt
      721 cgcggcggtc agccactgct atgagcggcc acctttgccc aggctgcccc acaatctcta
      781 cgccatggtc cacgcgcatc acgacgacga gtgtcgggca ttggcggccc agatggcccg
      841 cgccatcggc gtccgggact acctcgcgct gttcacggtg accgagtata aaaaggcgag
      901 cccggtgtac tttgcggagg cagccggcaa ccggtgacgg tcactgctcg cgcggtgggg
      961 gagacgggtg acccggagag ggcggcgact gccggcgatt cgtccctaca gcttccgccc
     1021 cacggccgcc gcgataggct tgagctcggc catgagcgtc tcgaatgtct cgcacttgag
     1081 cgactgggcc ccgtcgcagt aggcttcctt cgggcgcggg tggacctcga tgacgagccc
     1141 atcggcaccg gccgccagcg ctgctttcgc catggcgggc acggcccacc agtggcctgt
     1201 ggcatggctg ggatccacca ggatgggcag gtgcgagtag cgccgaatca ccgccacggc
     1261 gctcaggtcg agtgtgtagc gtgtcgacgg ctcgaaggtg cgaacacccc gctcgcatag
     1321 cagcagccgc tcgttgccct cggagagcac gtactcggcg gccatcagca ggtcctcgat
     1381 cgtcgaggcc atgccgcgtt tgagtagcac cgctcgcccg gacttgccga cctcggtgag
     1441 cagggggaag ttctgcatgt tccgggtacc gatctgcagg atgtcgcagt gctcggccac
     1501 ggtctcgacg tcgcgaggg
//
LOCUS       DAOSPL010001334         1518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_83294_length_1518_cov_2.221463, whole genome shotgun sequence.
ACCESSION   DAOSPL010001334 DAOSPL010000000
VERSION     DAOSPL010001334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83294_length_1518_cov_2.221463"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..743
                     /locus_tag="PLQ54_19495"
     CDS             111..743
                     /locus_tag="PLQ54_19495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95506.1"
                     /translation="MRVRRRILTALLVLGLTACATRDGTAEPAAPQVPTKTDGAVIDV
                     VGWVPNNLTEMTNSADIVVVAVASRVGPGFTAADEKPPMSYTPVTWRVIEVIGEWETP
                     VDHPGAGRRGVPEPLVTWQMGQPGSPIEVIPQQGQTALLFLSRYEGNERTPSGVAAHT
                     AVSVSIYEGVYPLDSASKIPRTSSVCKGVCAEIGGLGIAEVKAKVKAARK"
     gene            876..>1518
                     /locus_tag="PLQ54_19500"
     CDS             876..>1518
                     /locus_tag="PLQ54_19500"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002534437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOS95507.1"
                     /translation="MTTTQLPEIEKIGAGKRIGILTSGGDAQGMNAAVRAVVRTAVTL
                     GAQVYAVYEGYQGMCDGGDGIRELGWEDVGSILHRGGTVIGTFRSKDFRDREGRKRAA
                     ANLLHHGIDRLVVIGGDGSLSGLDQFRAEWGELLAELVASGEITQETADAHPALMFAG
                     LVGSIDNDLVGTDMTIGADTALHRIVDAIDALASTAASHQRSFVVEVMGRHCGY"
BASE COUNT          263 a          502 c          526 g          227 t
ORIGIN      
        1 cggcatcatc ggggcggcgt gcgaggcgag tgccctgttc atgagcgcca gagcatcgat
       61 cgggtagtaa cctcggattg atttcaactt aggtagaagc gggaatcact atgcgtgtcc
      121 ggagacgcat cctgacagcc cttctcgtct tggggctgac ggcgtgcgca actcgcgatg
      181 ggaccgccga accggctgca ccgcaggtgc cgaccaagac cgatggggcc gtcatcgatg
      241 tcgtggggtg ggttccgaac aacctgacag agatgaccaa cagcgctgac atcgtggtcg
      301 ttgcggtcgc gagccgtgtg ggaccgggat tcacggcagc cgacgagaag ccgccgatgt
      361 cgtacacccc agtcacctgg cgagtcatcg aggtcatcgg ggagtgggag acaccggtcg
      421 accatcccgg ggcgggtcgg cgcggcgttc ccgaaccact cgtcacatgg cagatggggc
      481 agccaggctc ccctatcgag gtcattcccc agcaggggca gaccgcactg ctgttcctga
      541 gccgatatga gggaaacgag cgcacccctt cgggcgtggc ggcacacacc gctgtctcgg
      601 tgagcatcta tgagggcgtc tatccgctcg actcggcatc gaagatcccc cggaccagct
      661 ccgtgtgcaa gggggtttgc gccgagatcg gcggcctggg tatcgccgag gtcaaggcca
      721 aggtgaaggc cgcccggaag tagcaccccg cgggctgctc cctggggatg gaagcactga
      781 agcggtggtg acgaccacac gcccatcacg accagagggc ggcccaactt ctccccgccc
      841 gcgcgcggcc gggctgccag cgccagaatg tcggcatgac gactacgcag ctcccggaga
      901 tcgagaagat cggggccggc aagcgcatcg gcatcctcac cagcggcggt gacgcgcagg
      961 ggatgaatgc ggccgtacgg gcggtcgtcc gcacggccgt cacgctcggg gcgcaggtct
     1021 atgccgtgta cgagggctac caggggatgt gcgacggcgg cgacggcatc cgcgagctcg
     1081 gctgggagga cgtcggcagc atcctccacc gcggcggcac ggtcatcggc accttccgct
     1141 ccaaggactt ccgcgaccgc gagggccgca aacgggccgc ggcgaacctc ctccaccacg
     1201 gcatcgaccg cctcgtcgtc atcggcggcg acggctcgct gtccggcctc gatcagttcc
     1261 gcgccgagtg gggcgagctg ctggccgagc tggtggcgtc tggtgagatc acccaggaga
     1321 cggccgacgc ccaccccgcg ctgatgttcg ccgggctggt cggctcgatc gacaacgacc
     1381 tggtcggcac ggacatgacg atcggcgccg acaccgccct gcatcggatc gttgatgcga
     1441 tcgacgccct cgcgagcacc gccgccagcc atcagcgcag cttcgtcgtc gaggtcatgg
     1501 gccggcactg cggctacc
//
LOCUS       DAOSPL010001335         1517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_83328_length_1517_cov_3.798222, whole genome shotgun sequence.
ACCESSION   DAOSPL010001335 DAOSPL010000000
VERSION     DAOSPL010001335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83328_length_1517_cov_3.798222"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /gene="mnmG"
                     /locus_tag="PLQ54_19505"
     CDS             complement(<1..1493)
                     /gene="mnmG"
                     /locus_tag="PLQ54_19505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOS95508.1"
                     /translation="MSRIAVGDRGVEGVVLATGAAFRSRSVVVAAGTFMRGEVFIGHE
                     SFRLGRAGEPASDELPGSLEAAGIRLGRLKTGTVPRVHRGSIDDARCECQPSDSSGLR
                     MSFLTRRPRTRGLLPCLLTWTTPETHRLVREALPLSALYGGLISGTGPRYCPSIESKL
                     VRFPDKERHQVFLEQEGWTTAEVYVWGLSSSLPEDVQRDMLHSVPGLERAQIMRPGYA
                     VEYDYADPRQLGYTFEARAVPGLFLAGQVNGTSGYEEAAVQGLLAGANAVLVPRGCEP
                     LVPKRDEAYAGVLLDDLMARGVDEPYRMMTSRAEFRLRLRESNADLRLTPLGRRVGLV
                     DDRRWRAFLRRRRAIDQELEALESSSVVPDDESDAWADEVRTARLSASATAARLLERP
                     EVRYEHVVRLLGRRPLGPEETREVESRVKYRGYEAIEGVRVDRVRALEAQRLPDELDY
                     GSVHGLKSEAREKLGAARPRTLGQAARLPGVTASDVAVLAVALRARAG"
BASE COUNT          256 a          531 c          521 g          209 t
ORIGIN      
        1 ccagcgcggg ccctgagcgc aacggccaga accgccacat cggaggccgt caccccgggc
       61 aggcgggcgg cttgtcccag ggtcctcgga cgcgcggcgc cgagcttctc tcgcgcctcc
      121 gacttcaggc cgtgcacgct gccgtagtcc agctcgtcag gtagccgctg ggcctccagg
      181 gcgcgcacac ggtccacccg gacaccctcg atggcctcat agcccctgta cttcacccgc
      241 gactccacct cgcgcgtctc ctcgggcccg agaggccggc ggccaagcag gcggaccaca
      301 tgttcgtagc gcacctcagg cctctcgaga agacgggccg cagtggcact ggccgagagg
      361 cgagcggtgc ggacctcatc ggcccaagca tcggattcgt cgtcggggac gaccgaagac
      421 gactcgagtg cctcgagttc ttgatcgatg gccctgcgac gacgaaggaa ggcgcgccag
      481 cgccggtcgt cgacgagccc aacgcggcgc ccgaggggtg tcagacgaag gtcggcgttg
      541 ctctcccgga gccgaagccg gaactccgcg cgcgaggtca tcatgcggta gggctcatcc
      601 accccgcggg ccatgaggtc atcgagaagc acgccggcat aggcctcgtc acgcttgggc
      661 accagcggct cgcacccgcg gggaaccaga acggcgttgg cgccggccag caggccctgt
      721 acggcggctt cctcatagcc cgaggtgccg ttgacttggc cggcgaggaa cagcccaggc
      781 acggcccggg cctcgaaggt gtagcctagc tgccgcgggt ccgcatagtc atactcgacg
      841 gcatagccgg gacgcatgat ctgggctcgt tcgagcccgg gtaccgaatg gagcatgtcg
      901 cgctggacgt cctcggggag gctgctcgag aggccccaga cgtacacctc cgccgtggtc
      961 cagccctcct gctcgaggaa gacctggtgg cgctccttgt ctggaaaacg cacgagtttg
     1021 gactctatgg aagggcagta gcgtggcccc gtcccactga tgaggccgcc gtagagcgcc
     1081 gagagcggca gcgcctcgcg caccagccgg tgtgtctcgg gcgtggtcca cgtcagcaga
     1141 cacggcagaa ggcctcgagt ccgggggcgc cgcgtgagga aggacatccg gagcccgctg
     1201 gagtccgaag gttggcactc gcaacgtgca tcgtcgatgg acccccgatg gacccgcggc
     1261 acggtgccgg tcttgagccg gccgagacgg atgccggctg cctcgagaga cccaggcagt
     1321 tcatccgagg ctggctcgcc ggcccgtccg agccgaaagg actcgtggcc gatgaagacc
     1381 tcgcctctca tgaaggttcc ggccgcgacg accaccgatc gcgatcggaa tgcggccccc
     1441 gtggcgagca ccacgccctc gacaccccga tcgccgacgg caatgcggct cacggatccc
     1501 tggcggagag caaggcc
//
LOCUS       DAOSPL010001336         1515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_83485_length_1515_cov_3.127397, whole genome shotgun sequence.
ACCESSION   DAOSPL010001336 DAOSPL010000000
VERSION     DAOSPL010001336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83485_length_1515_cov_3.127397"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PLQ54_19510"
     CDS             <1..946
                     /locus_tag="PLQ54_19510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HOS95509.1"
                     /translation="PVPADVTALGLQSGVPVTYGASSTPDSEYNALVFADSLNSAGLP
                     ITASTDGTGGIQIVSSVPGVAGNFTLADQTGSAIADYFGSSSVNGGVDVARSSVVVYD
                     SLGVAHTVDVEFSRIGSSRQWEWTATDESGATVGSGSLNFSSDGQPLTPTGVIQLTLT
                     NPQATSPQIITLHFDNVQQFAGQSTAQVANQDGVAPGTLQTFSVSQDGVIVGEFSNGL
                     SQAVGQVALATFQNNGGLVRLGSTLFRETVNSGAAQVGTAGSAQRGTIQGGTLEASNV
                     DLADQFTKLIIAERGFQANSRIMTTSDEILQELVNLKR"
     gene            1017..1220
                     /locus_tag="PLQ54_19515"
     CDS             1017..1220
                     /locus_tag="PLQ54_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HOS95510.1"
                     /translation="MIPVTRLNGTELYVNSDLVETVEATPDTVVTLTTGKKLVVLESV
                     DDVVERMVQFRRRINVALQPPSE"
BASE COUNT          269 a          494 c          488 g          264 t
ORIGIN      
        1 cccggtaccc gcggatgtca ccgccctcgg tctccagtcg ggagtcccgg tcacctacgg
       61 cgcttcgtcg acccccgact cggagtacaa tgccctggtg ttcgctgatt cgctcaactc
      121 cgccggactg ccgatcacgg cgtcgacgga tggaaccggc ggtatccaga tcgtctcgtc
      181 ggtgcccggt gtagccggga acttcaccct ggctgaccag acgggttcgg ccatcgccga
      241 ctacttcggc tcctcgtccg tcaacggtgg ggtcgacgtg gcccggtcca gcgtggtcgt
      301 ctatgattcc ctcggcgtgg cgcatacggt cgatgtcgag ttcagccgca tcggcagcag
      361 ccgccagtgg gagtggacgg ccaccgacga gagcggtgcc accgtcggct ccgggtcgct
      421 gaacttcagc tccgacggcc agcccctgac gcccactggt gtcatccagt tgaccctgac
      481 caacccccag gccacatcgc cgcaaatcat cacactgcac ttcgacaatg tgcagcagtt
      541 cgcgggacag agcaccgccc aggtggcaaa ccaggacggt gtcgctccgg gaaccctgca
      601 gacattctcg gtgtcgcagg atggcgtgat cgtcggcgag ttctccaatg gcctgagcca
      661 ggccgtgggg caggtggctc tggccacctt ccagaacaac ggcggcctgg tccgcctggg
      721 cagcaccttg ttccgggaga ctgtgaactc gggagcggct caggtcggaa ctgccggctc
      781 ggcgcagcgc ggtaccattc aggggggtac cctggaggcg tcgaatgtgg atctggccga
      841 ccagttcacc aagctgatca ttgcggaacg cgggttccag gcgaactcgc gcattatgac
      901 cacctccgac gagatcctgc aggagctggt caacctgaag cggtagccct gaacaacggc
      961 gctggcgtgg ctgcgggatg gccccgcggc cgcgccagcc ctcaggagga gctccgatga
     1021 tcccggtgac gcgcctcaac ggcaccgagc tctatgtcaa ctccgacctc gtcgagaccg
     1081 tcgaagccac gccggacacg gtagtcaccc tcaccaccgg gaagaagctc gtggtcctcg
     1141 agtccgtcga tgacgtggtg gagcggatgg tccagttccg gcggcgcatc aatgtggccc
     1201 ttcagccccc cagcgagtga gcccgtttcc gtgcaggtga tcgcgccgcg cattcggaaa
     1261 acagatgctg ctgacacaaa gacactgagt tggatatcct ggtgacctgc tctccatgac
     1321 gaaaggaagg ccttagccgg tggatgagat ctccgaagag ggtgcgagcg gttcttccgg
     1381 gggcccgaag cagctctcgc tcatgcccgt catgatcctg ctggtcctgg tctgcatcct
     1441 catggcagcg aatctctacg tcagcatgac ccggaggaac ggaggaggcg gcggcgaggc
     1501 taagggcaag gggcc
//
LOCUS       DAOSPL010001337         1514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_83602_length_1514_cov_1.941056, whole genome shotgun sequence.
ACCESSION   DAOSPL010001337 DAOSPL010000000
VERSION     DAOSPL010001337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83602_length_1514_cov_1.941056"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="PLQ54_19520"
     CDS             complement(<1..912)
                     /locus_tag="PLQ54_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOS95511.1"
                     /translation="MLSRIRNIGISAHIDSGKTTLSERLLFYSGRIYRMGEVKGDAAT
                     ATMDFMDLEKERGITITSAATQIRWRDHTVNLIDTPGHVDFTIEVERSLRVLDGAVMV
                     LCAVGGVQSQSVTVDRQMKRYRVPRIAFINKMDRTGADPQRVVRDMAEKLGLNVVPIQ
                     LNMGDGADFQGVIDLVEMKALRFEGDNGEKVVVDEIPAEYVGPAAEAREHMLDALSMF
                     NDDLMMRMLEDHEIPVELLRDTLRAATINRDIVPVMMGSAYHNQGVQPLMDAVCRYLP
                     SPLDRQAFARDHDNEGAFDAAGSCCRRG"
     gene            complement(1206..>1514)
                     /locus_tag="PLQ54_19525"
     CDS             complement(1206..>1514)
                     /locus_tag="PLQ54_19525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95512.1"
                     /translation="CADAQNELWRIARAGKPAKLFERSPGHYKEWFMAITGEKPLDFP
                     KSNFHYAAPMTATIQLGNIALKCGKRLEWDGVKGEFTNDKNANQYLTKQYPAGWKFDV
                     "
BASE COUNT          244 a          467 c          513 g          290 t
ORIGIN      
        1 cccgcgccga caacagctgc cagccgcatc gaaggcgcct tcgttgtcgt ggtctcgggc
       61 gaaggcctgg cggtccagcg ggctgggcag gtagcggcag acggcgtcca tcagcggctg
      121 gacgccctgg ttgtggtagg ccgatcccat catgaccggg acgatgtcgc ggttgatggt
      181 ggcggcgcgg agggtgtcgc ggagcagttc gacggggatc tcgtggtcct cgagcatccg
      241 catcatgagg tcgtcattga acatgctcag ggcatcgagc atgtgttcgc gggcctcggc
      301 ggccgggccg acgtactcgg cggggatctc gtcgacgacg accttctcgc cgttgtcgcc
      361 ctcgaagcgc agggccttca tctccaccag gtcgatcacg ccctggaagt cggcgccgtc
      421 gcccatgttc aactggatgg gcacgacgtt gaggccgagc ttctcggcca tgtcgcggac
      481 cacccgctgc gggtcggccc cggtgcggtc catcttgttg atgaaggcaa tgcgcggcac
      541 gcggtagcgc ttcatctggc ggtcgacggt gaccgactgg ctctgcacgc cgcccaccgc
      601 acagaggacc atgaccgccc cgtccagcac gcggagcgac cgctcgacct cgatggtgaa
      661 gtcgacgtgg cccggcgtgt cgatcaggtt gaccgtgtgg tcccgccagc ggatctgggt
      721 ggccgcggag gtgatggtga tgccgcgctc cttctccagg tccatgaagt ccatggtggc
      781 ggtggcggcg tcgcccttga cctcgcccat ccggtagatc cggcccgaat agaacagcag
      841 gcgctcgctc aaggtggtct tgcccgagtc aatgtgcgcc gatattccga tgttgcgaat
      901 cctgctcagc atacctgtac ccatagccca caaaaacggg gttcgttaca cgtcctccag
      961 aggcaactac ggtgagagcc tacccgaagg taagtaagaa gtcctatatc gtatcaggat
     1021 tatcggccta tgtcaatatg cgacatgaga cttaactcaa cgttagcgcg ggcgatggac
     1081 cccgcccgcc atgcgcccgg aaggctgccc cccccggggg ccggagcttc ccgaaagaag
     1141 agcccgcgga ggggcgctcc acgggctctt gtctgcagaa cggattgtcg atcgggccgc
     1201 cgggcctaga cgtcgaactt ccagccggcg gggtactgct tggtcaggta ctggttggcg
     1261 ttcttgtcgt tggtgaactc gcccttgacg ccgtcccact ccaggcgctt gccgcacttg
     1321 agggcgatgt tgccgagctg gatggtcgcg gtcatcgggg cggcgtagtg gaagttggac
     1381 ttggggaagt ccaggggctt ctcgccggtg atggccatga accactcctt gtagtggccc
     1441 ggcgagcgtt cgaacagctt ggccggcttg ccggcgcggg cgattcgcca taattcgttt
     1501 tgggcatcgg cgca
//
LOCUS       DAOSPL010001338         1513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_83673_length_1513_cov_2.479424, whole genome shotgun sequence.
ACCESSION   DAOSPL010001338 DAOSPL010000000
VERSION     DAOSPL010001338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83673_length_1513_cov_2.479424"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PLQ54_19530"
     CDS             complement(<1..396)
                     /locus_tag="PLQ54_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HOS95513.1"
                     /translation="MLDQDLLDLGLRFHGHRCPAMPLGLRTALAAMNALGVEHAPDAQ
                     LVALIEIDSDHCATCWADGVQVATGCTLGKGNIRKLGYGKWGLTLIDKATSRAVRVVP
                     KAAAMQKSKESDFMALRKSGTPASRVPPEV"
     gene            complement(494..1192)
                     /locus_tag="PLQ54_19535"
     CDS             complement(494..1192)
                     /locus_tag="PLQ54_19535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOS95514.1"
                     /translation="MSDLLAALSRAVEGAPALALGASLVWGVLSIVLSPCHLASIPLI
                     VGFIDEQGRSTTRHAFVLSLLFGTGILITIALIGAITAALGRLMGDVGPWGTHLTVTI
                     MLIVGLHLLGAIPLNLRGADASGFSRRGLLAAFILGLVFGIALGPCTFAYMAPMLAVT
                     FRTSAETPLYGVALLLAYGIGHCGIIVAAGTSTELVHKWLHWTEASRGAVVLKSVCGV
                     LVILGALYLLWGAL"
     gene            complement(1275..>1513)
                     /locus_tag="PLQ54_19540"
     CDS             complement(1275..>1513)
                     /locus_tag="PLQ54_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOS95515.1"
                     /translation="MAPILEELKTEYEGRLIVEFIDIYADPASAEKHGIMVIPTQIFL
                     DADGKELFRHEGFFAKDEILAKWKELGVDLGNAP"
BASE COUNT          270 a          495 c          514 g          234 t
ORIGIN      
        1 cacctccggc ggtaccctgg aggccggagt tccagacttg cgcagcgcca tgaagtccga
       61 ctccttgctc ttctgcatcg ccgcggcctt gggaaccacg cggacggccc tcgaggtcgc
      121 tttgtcgatc agcgtgagcc cccacttccc gtagccaagc ttccggatgt tccccttgcc
      181 gagagtgcat ccggttgcca cctggacgcc gtcagcccag caggtggcgc agtggtcgct
      241 gtcgatctcg atcagtgcca cgagctgcgc gtccggggcg tgctccaccc ccagcgcgtt
      301 catcgcggcc agtgccgtgc gcagaccgag tggcatcgcg gggcaccgat ggccatggaa
      361 cctgaggccg aggtccagca gatcctgatc caacatgaaa atgcctctct ctcccgcgcg
      421 tccggcgcgc gtatcgtggc cgctatgcgg cccacagatc gcccccaggc ccagccattg
      481 ctaccgccac ggttcatagc gcaccccaca gcaggtacag cgcgccgagg atgacgagca
      541 cgccgcagac gctcttgagg acgacggcgc ctcgggaggc ctcggtccag tggagccact
      601 tatgcaccag ttcggtcgac gtgccggcgg cgacgatgat accgcagtgg ccgatgccgt
      661 aggccagaag cagagccacc ccgtagagcg gcgtctccgc cgaggtgcgg aaggtgacgg
      721 cgagcatggg cgccatgtag gcgaaggtac acggccccag ggcgatgcca aacacgagac
      781 ccaggatgaa ggcggccaag aggccccggc ggctgaaccc gctggcgtcg gctccgcgca
      841 gattcagcgg gatggcgccc aggaggtgga ggccgacgat gagcatgatg gtcacggtga
      901 ggtgggtacc ccaggggccc acatcaccca tgagtctgcc gagggcggcc gtgatggcgc
      961 cgatcagggc gatggtgatg aggatgcccg ttccgaatag gagcgacagc acgaaggcgt
     1021 gccgggtggt gctgcggccc tgttcatcga tgaaccccac gatgagcggg atgctcgcca
     1081 ggtggcaggg gctgagcacg atgctcagga cgccccagac gagtgacgcg cccagcgcca
     1141 gcgccggtgc gccctcaacg gcacggctga gcgcggcgag caggtcggac atcggggctc
     1201 ctcccggaaa tcaggggtca ggggtcagaa ctccgaaagc ggggagagag agctgggcga
     1261 ccttccggtc gcccctacgg agcgttgccc aggtccacgc cgagctcttt ccacttggcg
     1321 agaatctcgt ctttcgcgaa gaacccctcg tgccggaaca gctccttgcc gtcggcgtcg
     1381 aggaagatct gcgtgggaat caccatgatg ccatgcttct cggcggaggc cgggtccgcg
     1441 tagatgtcga tgaactcgac gatgagccgg ccctcgtact cggtcttcag ctcctcaagg
     1501 atgggagcca ttt
//
LOCUS       DAOSPL010001339         1511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_83802_length_1511_cov_2.876374, whole genome shotgun sequence.
ACCESSION   DAOSPL010001339 DAOSPL010000000
VERSION     DAOSPL010001339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83802_length_1511_cov_2.876374"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="PLQ54_19545"
     CDS             <1..1042
                     /locus_tag="PLQ54_19545"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOS95516.1"
                     /translation="LKAVEQLDYGDVLAAPVLGPGPDPFLPSGQVVGPTVLQALEQAG
                     VSRVYVEDPRADDVEISDVLSPETAAACRAAVTRALGGTPRVSDLRDAARRLVQDVRR
                     GAPPLLAAVSIHHLAAYPAVHAVDSAKTAVLLGRELNYTDQQLQNLAAGMLVHDIGNQ
                     RVPQGLLEASRELSEVERRETQVHARAGFEMLRQVLSATPLGRSLALYHHERLDGSGY
                     PRGVKGDEIPEFGRIAAIADVYAALTLDRHHRPALAPDRAFAHLLARQGKFDARIAAL
                     LARNVAIYPPGMPVELDSGEIAVVARVAPGNTLHPVVRLWTTPGGLPFQSPQDLDLNL
                     HHDIRIVRSKLI"
     gene            1073..>1511
                     /locus_tag="PLQ54_19550"
     CDS             1073..>1511
                     /locus_tag="PLQ54_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOS95517.1"
                     /translation="MPESVPLISRHVLFGNPERAAPSLSPNGRWIAFLAPLDGVLNVW
                     LAEAADPAAARAITHDRGRGIQWYEWAQTSEHILYVQDSDGDENWHVYRVGVEGGDPA
                     DLTPLESIHAQVQHVSHRFPDEILVSLNDRDPRWHDIYRVNILT"
BASE COUNT          239 a          525 c          507 g          240 t
ORIGIN      
        1 tctcaaggca gtcgagcagc tggactatgg tgacgtgttg gcggcccctg ttcttgggcc
       61 gggccccgat ccgttcctgc cctcgggcca ggtggtggga ccgacagtgc tgcaggcgct
      121 ggagcaggct ggggtctcgc gggtatacgt ggaggatccg agggcagatg atgtcgagat
      181 ctcggacgtc ctatcgcccg agacggcggc cgcgtgccgg gccgcagtga cccgggcctt
      241 ggggggcacc ccccgcgtgt cggatctgcg ggacgcggcg cgcaggctgg tgcaggatgt
      301 ccgccgggga gcgccgccgc tcctggcggc cgtcagcatt caccacctgg cggcctaccc
      361 ggccgtgcat gcggtagatt cggctaagac ggccgtgctc cttggccgcg agctcaacta
      421 caccgaccag cagcttcaga acctcgctgc ggggatgctg gtccacgaca ttggcaacca
      481 gcgggtgccc cagggcctgc tcgaagccag ccgcgagctg tcggaggttg agcggcgcga
      541 gacccaagtc cacgcgcgag cgggattcga gatgctgagg caggtgctct cagccacgcc
      601 gctggggcgg agcctggccc tctaccatca cgagcggttg gacgggagcg gctacccacg
      661 cggggtgaag ggcgacgaga tccccgagtt cggccgcatc gcggccatcg ccgacgtgta
      721 cgccgctctg acactggatc gccaccaccg ccccgcgctg gccccggacc gggcattcgc
      781 gcacctgctc gctcgacagg gcaagttcga tgcccggatc gccgccctgc tggcgcgtaa
      841 tgtggccatc tacccaccgg gcatgcccgt cgagctggac tctggcgaga tcgcggtcgt
      901 cgctcgcgtc gctcccggca acacgctcca ccccgtggtg cggctctgga ccacgccagg
      961 agggctgccc ttccaatcgc ctcaggacct ggatctgaac ctgcaccacg acattcgcat
     1021 cgtccgctcc aaactcatct gacgcgtcaa ctcgcccgaa cggaggcact ccatgcctga
     1081 gtccgtcccc ctgatctcgc gccacgtcct gtttggcaac cccgaacggg ccgctccgag
     1141 tctgagtccc aacgggcggt ggatcgcgtt cctggctcct ctggacggcg tactgaatgt
     1201 gtggctggcc gaggcggccg atccggccgc cgcacgggcc attacgcacg acagggggcg
     1261 cggcatccag tggtatgaat gggcgcagac ttccgagcat atcctgtacg tgcaggacag
     1321 cgacggcgac gagaactggc acgtgtaccg cgtgggcgtc gaaggcggtg acccggcaga
     1381 cctgacgccg ctggagagca tccacgccca ggttcagcac gtcagtcacc ggttccccga
     1441 cgagatcctg gtcagtctga acgaccgcga tccgcgctgg cacgacatct accgcgtcaa
     1501 catcctcacc g
//
LOCUS       DAOSPL010001340         1509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_83944_length_1509_cov_3.689821, whole genome shotgun sequence.
ACCESSION   DAOSPL010001340 DAOSPL010000000
VERSION     DAOSPL010001340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83944_length_1509_cov_3.689821"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PLQ54_19555"
     CDS             complement(<1..724)
                     /locus_tag="PLQ54_19555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95518.1"
                     /translation="MLVARWIICLLTVGVLEAAACPAAPQPAAPQPSMPAQEALATLA
                     EFLHIDVADITLVREGQTGRSEAAFGGLVRHKPPTDGPWWGPPDAAGLVECRLEVGRE
                     RGTGKPYVVLADWAIVGRSFGTPAISLEAVPDLARAFLEAYCPYLDDSVKMTYFKGFP
                     DGESDVGMTGWTRETATTSLWIQADVRLDDGSISYGCRYRTGLTPAAITEDQARQIAE
                     EQWRAKYPEQELVYEKTSKLLES"
     gene            complement(730..>1509)
                     /locus_tag="PLQ54_19560"
     CDS             complement(730..>1509)
                     /locus_tag="PLQ54_19560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95519.1"
                     /translation="FVATGVQNATSALNNRSRAAKWLKPIAKPFEEWPTTYSLAGHGV
                     NNTPEEPLEAQAVAANGALAAYVSEQGYHYGAQLGDNDTATLYGKLPTSSVVFIAAHG
                     APNRQVMENGCDPLRDEIRGGTASADPPGNDPARDIYYIEELADGMGHCRFVFFAGCD
                     TADRNEAGESLVENAVAKGADLAAGFRAYEYAGLPAVVMFYDRFWHHALVGGQSVKDA
                     AVAAIGEARNAYGNDALGGVDTFYLYPRDSEENLSQVEWGD"
BASE COUNT          233 a          523 c          483 g          270 t
ORIGIN      
        1 cggattccag tagcttggag gtcttctcgt agaccagctc ctgctcgggg tacttggcgc
       61 gccactgctc ctccgcgatc tgcctggcct gatcctcggt gatggcggcg ggcgtcagcc
      121 cagtgcgata ccggcagccg tacgaaatgc tgccatcgtc aaggcggaca tcggcctgga
      181 tccacagaga cgtggtggcg gtctcgcgcg tccagccggt catccccacg tcgctctccc
      241 cgtcggggaa gcctttgaag taagtcatct tcacactgtc gtcgaggtac gggcagtagg
      301 cctccaggaa cgcgcgtgcc agatccggca cggcctcgag ggagatcgcc ggtgtgccga
      361 aggacctccc cactatcgcc cagtccgcga ggaccacgta gggcttgccg gtcccgcgct
      421 cgcgtccaac ctcgagccgg cactccacca gcccggccgc gtccggcggc ccccaccacg
      481 gaccgtctgt aggcggcttg tggcgcacga gaccgccgaa ggccgcctcg ctcctgccag
      541 tctgaccctc ccggacgagc gtgatgtccg ccacgtcgat gtgcaggaac tcagcgagag
      601 tggccagggc ctcctgcgcg ggcatggaag gctggggtgc ggccggctgc ggtgccgcgg
      661 ggcacgcagc agcctcgagc acacccaccg taagtaggca gatgatccat cgagcgacca
      721 gcacgactct cagtcccccc actcgacttg cgacagattc tcctcgctat cccttgggta
      781 gaggtagaac gtgtccacgc ctcccagggc atcgttgccg taggcattcc tggcctcccc
      841 gatcgccgcc accgccgcgt ctttcacgct ctgtccgccg accagagcat gatgccagaa
      901 cctgtcgtag aacatgacga ccgccggcag tccggcgtac tcgtaggcgc ggaaaccggc
      961 ggcgaggtcc gcacccttgg cgaccgcgtt ctcgacgagt gattcgcccg cctcgttccg
     1021 gtcagccgtg tcgcaccccg cgaagaagac gaaccggcag tggcccatgc cgtcggccag
     1081 ctcctcgatg tagtagatat cgcgggccgg gtcgttacct ggtggatcgg cactggccgt
     1141 accgccccgg atctcgtcgc gtagcgggtc gcagccgttt tccatgacct gtcggtttgg
     1201 cgcgccgtga gcggcgatga acaccactga gctagtcggg agcttcccat agagcgtggc
     1261 cgtgtcgttg tcgccgagtt gcgccccgta gtggtacccc tgttcggaca cgtaggccgc
     1321 cagcgcgcca ttcgcggcca cggcctgggc ctcaagtggc tcttcaggtg tgttgttgac
     1381 gccgtggccg gcgagcgagt aggtcgtcgg ccactcctcg aacggtttgg cgatgggctt
     1441 cagccacttc gccgcccgag agcggttatt cagcgctgat gtggcgttct gaacccccgt
     1501 ggccacgaa
//
LOCUS       DAOSPL010001341         1509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_83963_length_1509_cov_2.783356, whole genome shotgun sequence.
ACCESSION   DAOSPL010001341 DAOSPL010000000
VERSION     DAOSPL010001341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83963_length_1509_cov_2.783356"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLQ54_19565"
     CDS             complement(<1..242)
                     /locus_tag="PLQ54_19565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95520.1"
                     /translation="MARRVALSVLLCIAVVCPVAAAADEPRAAGDGAADQWIAPLPDD
                     LDLGGPFGEVWRKEQMSRWVVWPSDEYIAERQPPKDR"
     gene            complement(322..1089)
                     /locus_tag="PLQ54_19570"
     CDS             complement(322..1089)
                     /locus_tag="PLQ54_19570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95521.1"
                     /translation="MPAVAALLWLGPVWAVDDAGDAAPAPEQWIAALPDDLGLPVWGW
                     REYGMARWVIWPSDDDVAERQPPRDRSEPVGDPDGQSGKPHELLCPPGNARSEALSWV
                     RELLKPEWAPPDLEDCLTLLQEEDPAESSVVCRFEIDGTGIQIAQRRWAMCVVVKPSP
                     ALSDGVPGDQMGPALMRALFARGDAMAEVPATDRHDLAPGLWLGGYVAGEGDGRGGGP
                     RWWHWKAWYTDGLGVAIYFSKLSFDRKTSYLPGNPWF"
     gene            complement(1141..>1509)
                     /locus_tag="PLQ54_19575"
     CDS             complement(1141..>1509)
                     /locus_tag="PLQ54_19575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95522.1"
                     /translation="APYPGEEGSTTNDYVSNYRDMRLKTKDVGGAFTALMFQDLPVYS
                     AHEGCLRVEDGSADDGNQWWTVWPFAAHGTAKDLLIWFVETRGHPQRWFVNGSGFQPG
                     TVDIPKDLIYPCPKILGGPN"
BASE COUNT          250 a          562 c          449 g          248 t
ORIGIN      
        1 cgatccttcg gcggctgtcg ttcggcgatg tactcgtccg atggccagac aacccaccgt
       61 gacatctgtt ccttgcgcca cacttccccg aaaggtccgc ccaggtccaa gtcgtccggc
      121 aatggcgcta tccactgatc agcggcgcca tcaccggcgg cccgtggctc gtcagccgcc
      181 gcggcaacag gacagacaac cgcaatgcag agcagtacac tcagcgcgac gcgccgtgcc
      241 actggacccg cctccttcgt tctccgtcaa ctaccgggcg gcggggccgg tgtctcgcca
      301 gccccgccgc ccacgccatg ctcagaacca ggggttgccg ggcaggtacg aggtcttccg
      361 gtcgaatgag agcttcgaga agtaaatcgc cacgcctagg ccgtcggtgt accaggcctt
      421 ccagtgccac catctggggc cgccgccccg gccgtcgcct tcgcccgcca catacccccc
      481 gagccacagg ccgggtgcca ggtcatgcct atcggtcgcc ggcacctcag ccatggcatc
      541 tccacgcgcg aagagcgctc gcatcagtgc cggacccatc tggtctcccg gcacaccgtc
      601 cgagagcgcc gggctcggct tcaccaccac gcacatcgcc caccttcgct gggcgatctg
      661 gatcccggtg ccatcgatct cgaatcggca gactaccgac gactcagccg ggtcctcctc
      721 ctgcaagagt gtgaggcagt cctccaaatc agggggcgcc cactcgggct tcagcagctc
      781 cctcacccat gatagtgcct cggagcgcgc gttacccggc gggcagagca actcgtgcgg
      841 tttgccgctt tggccatcgg ggtcaccgac cggctcggac cgatccctcg gtggctgccg
      901 ctcggcgaca tcgtcgtcgg acggccagat gacccaccga gccatcccgt actcgcgcca
      961 accccacacc ggcagcccca agtcgtccgg cagggcggcg atccattgtt cgggagcggg
     1021 tgcggcatcc ccggcgtcgt ccacagccca gaccggcccg agccacagca aagcagccac
     1081 tgcgggcaag actgctacca cttgcgaagc gcgcgtcctc gctgacatct gctcctcccc
     1141 ctagttgggc ccgcccagga tcttggggca ggggtagatc aggtccttcg ggatgtccac
     1201 cgtgccgggc tggaacccgg acccgttgac gaaccacctc tgagggtggc cccgcgtctc
     1261 gacgaaccag atcaggaggt ccttggccgt gccgtgggcc gcaaatggcc agaccgtcca
     1321 ccactggttg ccgtcgtcgg cgctcccgtc ctcaacgcgc aagcacccct cgtgcgcgct
     1381 gtagacgggc aagtcctgga acatcagagc cgtgaaagcg ccgccgacgt ccttggtctt
     1441 cagacgcatg tcacggtagt tgctcacgta gtcgttcgtt gtggaacctt cctcacccgg
     1501 gtagggtgc
//
LOCUS       DAOSPL010001342         1507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_84113_length_1507_cov_3.450413, whole genome shotgun sequence.
ACCESSION   DAOSPL010001342 DAOSPL010000000
VERSION     DAOSPL010001342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84113_length_1507_cov_3.450413"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="PLQ54_19580"
     CDS             <1..1019
                     /locus_tag="PLQ54_19580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95523.1"
                     /translation="IASAPWPTLTDFLSGDLATEGELVTLPMADGQGRRLLGPDVTWL
                     VLRGSVGGPSGGSLGFLWVFNAEVTPELLTQARLAPEPAAPTPSPALPAVVVRIPTYA
                     SGGRAVELPSEAAGDAIPAPAPAPALPEPPIAVIRGAESSTPRPIATLGPETARVSPA
                     RPASAWSEAPAAPNLSAGASEQEVYERLLPPLSRRTEVAGVPAVAIWFTEELIDSLLA
                     VVGDPADQAELSQLRRAAQTHFVFLVSAPEPEGPGSLPFAARCTLTDLLANRRRGLSL
                     TTVVPGAIGGSNKGGTYVCFSKLSRNKREMSVREANTFTLTVEIGRQVTPAVFTWRLP
                     LGRD"
     gene            1092..>1507
                     /locus_tag="PLQ54_19585"
     CDS             1092..>1507
                     /locus_tag="PLQ54_19585"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOS95524.1"
                     /translation="MAQHPSERRDASRLMVVDRRASTWSHARFSDLGDYLVPGDCLVL
                     NDTRVIPARLIGRRLTGGRAEALLLRPLPGGSWEALCRPASRLRPGTVIDFDAEGRGR
                     LTGEVTARGPEGICHLRLAHQGPLEAVLDAVGHVPLP"
BASE COUNT          210 a          532 c          522 g          243 t
ORIGIN      
        1 ccatcgcgag cgcaccctgg cccacactca ctgacttctt gtccggcgac ctggcgacgg
       61 agggtgagct ggtgactctg cccatggccg atggccaggg gagacgcctt ctgggccctg
      121 atgtcacctg gctggtgcta cgcgggtccg tcgggggccc gtcgggcggg tcgttggggt
      181 tcctgtgggt cttcaatgcg gaggtcacac cggagcttct gacccaagcg cggctggccc
      241 ccgagccggc ggcgcccacg ccctccccgg cccttcccgc ggtcgtcgta cggattccga
      301 cctatgcgtc aggaggccgg gcggtcgagc tcccatcgga ggccgcaggc gatgccattc
      361 cagcaccagc accagcaccc gccctgcccg agcctcccat cgcggtaatc cggggagcgg
      421 agagcagcac gcctcggccg atcgcgaccc tggggcccga gacggctcga gtgtctcccg
      481 cgaggcccgc atcggcctgg tcggaggccc cggctgcccc aaacctgagt gccggcgcgt
      541 cggagcagga ggtctatgag cgcctgctgc ctccactgag ccgccggacc gaggtggccg
      601 gagtgcctgc ggtggcgatc tggttcaccg aggagctcat cgatagcctg ctggcggtcg
      661 tgggagatcc agctgaccag gcggagctca gccagctccg gcgcgccgcg cagacccact
      721 ttgtcttcct ggtgagtgcg ccggagcccg agggaccggg atccctgccc ttcgccgctc
      781 ggtgtaccct gaccgatctg ctggccaacc ggcgcagggg gctttcgttg actacggtgg
      841 tgcccggcgc gatcgggggc agcaacaagg gcggcaccta cgtatgcttc tcgaagctga
      901 gccgaaacaa acgcgagatg tcggtccgcg aggccaacac cttcaccctg acggttgaga
      961 tcggtcggca agtcacgccc gcggtcttca cgtggcgact tcctcttggc cgagactagc
     1021 ctgcccggac ccgagcgagg tatgccgcga cggacatcgg cctcttcgac tacgagcttc
     1081 cgccggacct ggtggcccag cacccttccg agcgacgcga cgcatcgcgc ctgatggtcg
     1141 tcgatcggcg agcgtccaca tggagccacg cgcgcttctc cgacctgggc gactacctgg
     1201 tcccgggcga ttgccttgtg ctcaacgata cccgagtcat cccggcacgc ctgatcggcc
     1261 ggcggctaac gggaggtaga gccgaggccc tgctgctgcg ccctctcccg ggcggctcgt
     1321 gggaagcgtt gtgccgaccg gcctcgcggc ttaggccggg caccgtgatt gacttcgacg
     1381 ccgaaggccg ggggcgcctg accggcgagg tcacggcgcg cgggcctgag ggtatctgcc
     1441 atcttcgcct ggctcaccag gggccgctcg aggccgtgct ggacgccgtg gggcacgtgc
     1501 ctctgcc
//
LOCUS       DAOSPL010001343         1507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_84141_length_1507_cov_2.650826, whole genome shotgun sequence.
ACCESSION   DAOSPL010001343 DAOSPL010000000
VERSION     DAOSPL010001343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84141_length_1507_cov_2.650826"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PLQ54_19590"
     CDS             complement(<1..670)
                     /locus_tag="PLQ54_19590"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOS95525.1"
                     /translation="MTHAIMPALPRVTATTIWCSVLALSCYGQEEPKPAMLGAMRAKS
                     ILFLGNSVTLHGPAEQLGWPHHCGMAASAPENDYVHLLAAAIEARTSAPLRISPADSE
                     EAGTTRAANVVNVADIFERNYGTYTDERLRGQLDLKADIVVLQFGENIVRETFDPEAF
                     ASGLRTLVAGLKAHGDPHIFVTSQILGAGGPLDDIKREVCAEDPSHRVFVDLSQFGKD
                     PVNFA"
     gene            759..>1507
                     /locus_tag="PLQ54_19595"
     CDS             759..>1507
                     /locus_tag="PLQ54_19595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95526.1"
                     /translation="MAEMTSRERFQRMFEHREADRVPMIGGPWGTTLERWRREGMPAD
                     ADFADYFGLDQMVGVGGDVSPRYESRVIEETDEYVIAFDSWGTTSKNWKHASSTPHWL
                     GRTIVDRKTWEAAKARMVMGRDRIDWEGLRRGYPVWREKGWWIYGGLFFGFDVTHARI
                     VGTERLLMLMADDPELVADIYNTELDCSLQLLDMIWDEGYTFDAIFWCDDMGYRNGTF
                     FSVPMYRELLLPVQKRAIEWAHAKGIFAHLHS"
BASE COUNT          242 a          478 c          515 g          272 t
ORIGIN      
        1 tggcgaagtt taccgggtcc tttccgaact ggctcaggtc gacgaacact cggtgggatg
       61 ggtcttcagc gcacacctca cgcttgatgt catcgagcgg gccgccagcg ccgaggatct
      121 ggctcgtgac gaagatgtgc gggtcgccgt gcgccttgag tcccgccaca agtgtgcgta
      181 ggccgctcgc gaaggcctcc gggtcgaagg tctccctgac gatgttctcg ccaaactgca
      241 gcaccacgat gtcggccttc aggtcgagct gtccgcggag ccgctcgtcc gtgtaggtgc
      301 cgtagttcct ctcgaagatg tcggccacgt tcaccacgtt ggcagcccgg gtggtaccgg
      361 cctcttcgga gtccgcgggg ctgatccgca gcggcgcgct ggttcgagcc tcgatggcgg
      421 cggccagcag gtggacgtag tcattctccg gcgcgctggc ggccatcccg cagtggtgcg
      481 gccaccccag ctgctccgcc gggccatgta gcgtgacgct gttcccgagg aacaggatgc
      541 tctttgccct catcgcgccg agcatggcgg gcttcggctc ctcctggccg tagcaggaga
      601 gtgcgagcac gctgcaccag attgtggtgg cggtgacgcg gggcaacgcg ggcatgatgg
      661 cgtgggtcat agatggccta cctccccggg cccccaggat actcgctctc ggccggcgaa
      721 gacatggcgc tgttgtgcgc cacgacggag gcaggcacat ggctgagatg accagtcgcg
      781 agcggttcca gcggatgttc gagcaccgag aggccgatcg ggtccccatg atcggggggc
      841 cctggggcac gaccctcgag cgttggcgcc gagagggcat gccggccgac gcggatttcg
      901 ccgactactt tggcctcgac cagatggtcg gcgtcggcgg cgacgtgagt ccgcgctatg
      961 agtccagggt gatcgaggag acggacgagt acgtcatcgc cttcgactcc tggggcacga
     1021 cctcgaagaa ctggaagcac gccagctcga caccccactg gctcggccgc accatcgtgg
     1081 accggaagac ctgggaggcg gccaaagcgc ggatggtcat ggggcgggac cggatcgact
     1141 gggagggtct ccggcgcggc tatcccgtgt ggcgcgaaaa aggctggtgg atctacggcg
     1201 gcctgttctt cggtttcgat gtcacccacg cccgcattgt ggggaccgag cgactcctga
     1261 tgctgatggc tgacgacccc gagctggtgg cggacatcta caacaccgag ttggactgca
     1321 gcctccagct cctcgacatg atctgggacg agggctacac cttcgacgcc atcttctggt
     1381 gtgacgacat gggctaccgc aatgggacgt tcttctccgt ccccatgtac cgcgagctgc
     1441 ttctcccggt gcagaagcgc gccatcgagt gggctcacgc caagggcatt ttcgcccacc
     1501 tgcactc
//
LOCUS       DAOSPL010001344         1505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_84331_length_1505_cov_1.889655, whole genome shotgun sequence.
ACCESSION   DAOSPL010001344 DAOSPL010000000
VERSION     DAOSPL010001344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84331_length_1505_cov_1.889655"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..378)
                     /locus_tag="PLQ54_19600"
     CDS             complement(103..378)
                     /locus_tag="PLQ54_19600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95527.1"
                     /translation="MLRSLGVLAWGLALAVPTWAAWREGSTESGDAGTSVVLGAIAPG
                     IIKPPASSDGNNWWLIQGKSGQVQAQIMDTDYWDGPAPPPCGCYATP"
     gene            complement(640..>1505)
                     /locus_tag="PLQ54_19605"
     CDS             complement(640..>1505)
                     /locus_tag="PLQ54_19605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95528.1"
                     /translation="AVQRDEKLRRLCVRADGSTSRALNLCTADSVAEDSRRRTFVYSA
                     TDSVMLILDAEGTPILDEYDEEGELVAYGGWAVLEAGVAALAGERHWDRIHFDSVAAD
                     TSGDLYLRCASEGYLQKWECFLLAVDPERLAITSLIPAPWPTLGGAALRLDDVSPGVL
                     NLYVLDRRGSETGSMAIRSPREVMGAGGKPAGASAVGAAWLDAAGRPILKWGDRPDCL
                     VAHSRETGDALAWAPLPRHERQGVYSGLPQQAPGGGIWWVHFGADSLRVLCFPASAAA
                     GEGGGLSHTPD"
BASE COUNT          234 a          557 c          489 g          225 t
ORIGIN      
        1 ccgcctgccc cgtgaagtcg ctcgccgcag tgaaggtggg catggcggcg gtcacactgc
       61 tgaaactgcc cgcctctggg ggctcgacgc tccagtggta cgtcacgggg tcgcataaca
      121 cccgcacggc ggcggcgcag gaccgtccca gtagtcggtg tccatgatct gcgcctggac
      181 ctggcctgac ttgccctgga tcagccacca gttgttccca tcagaggacg ccggcggctt
      241 gatgatcccc ggcgcgatgg cgcccaggac gacactggtg cccgcgtccc cgctctcggt
      301 gcttccttcg cgccacgctg cccacgtcgg aaccgccagt gcgagtcccc atgccagcac
      361 tcctaggctt cggagcatgc tatgagtcgt ctcccttcgc ggtgaccacc ggtatggtcc
      421 agcctcccct ggccgcgtcc cagtcgcgcg cagaccaacc acgcacagac caccgtgccc
      481 acacattgtg ggtcggcaac cagagagcgt cctcagcggt tgcgtggacg gccactcagg
      541 tgagcccggg gccggatcgg ggttggcccc cgcgtccggg ggcacgcagc ggaccaatgg
      601 ccctggtggt atcattggct gcctacgggg cagccccacc tagtcaggcg tgtggctcag
      661 accacccccc tcgcccgcag cggcactggc cggaaagcag agcacacgca gggagtcggc
      721 cccgaagtgg acccaccaga tgccgccccc cggggcctgc tgggggagcc cggagtacac
      781 gccttgccgc tcgtggcgtg gcagaggcgc ccacgcgagc gcatcgccgg tctctcgcga
      841 gtgcgccacc aggcagtctg gccggtcgcc ccacttcagg atcggcctgc cggctgcgtc
      901 cagccacgcc gccccaaccg ccgaggcgcc cgcaggctta ccgcccgcgc ccatgacctc
      961 gcgcgggctg cgaatagcca tgctcccggt ctcgcttccg cgacggtcga gaacgtacag
     1021 gttgaggaca cccgggctta cgtcgtcgag tcgaagcgct gcgcccccca gcgtgggcca
     1081 cggcgccggg atgaggctgg tgatcgcgag ccgctccggg tcgactgcca gaaggaagca
     1141 ctcccatttc tgcaggtagc cctcggacgc acagcgcagg tacagatcgc ccgatgtgtc
     1201 tgccgcgacg ctgtcgaagt ggatccggtc ccagtgccgc tcacccgcga gggccgcgac
     1261 ccccgcctcg aggacggccc aaccgccgta ggcgaccaac tcgccctcct cgtcgtactc
     1321 gtccaggatg ggcgtaccct cggcgtcgag gatcagcatg acgctatcgg tcgcgctgta
     1381 gacaaaggtg cggcgacgcg agtcctcggc taccgaatcg gcggtgcaga ggttgagcgc
     1441 gcggctcgtc gacccatcag cccgcacgca gagacgtcgt agcttctcat cacgctgcac
     1501 ggcca
//
LOCUS       DAOSPL010001345         1504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_84364_length_1504_cov_3.130435, whole genome shotgun sequence.
ACCESSION   DAOSPL010001345 DAOSPL010000000
VERSION     DAOSPL010001345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84364_length_1504_cov_3.130435"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="PLQ54_19610"
     CDS             complement(<1..1446)
                     /locus_tag="PLQ54_19610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95529.1"
                     /translation="MRVYPTAFGWFGWTVDKALGEPTVSEDGRRWTYRSDPTELMDTS
                     YSTRVPAATEMVAVFGSGELPVPRLRVSGPSTGVWAPMDLEIEWGFRPGTERKPVSGR
                     LETHVAVIGTVAPLRGDATTTVMGGARWRSAAEHRSPERRGLRVSLLYAPTIRPGLDS
                     RVTLWTDAGDVTFSVKDLESGPIYLPRQGLFISRAGSAETGAAFAARLEAEGRKSLRQ
                     MTREHREPRSWEELMEQVRFWTCPEGTPVPPFPEVPDPAMTVELSDARWTGAWRTACD
                     ELRGRHLWPTLSAEIARAARVMELVGLHDEVMPVYDYFLGSPGVKSDGDFTDPAGSLE
                     WATSMRHDMGYSHEGTHASTGRLLWAMCDRYFLTGDRDWFAAHRERLQAAADWIIRER
                     DRYMEDCPNRDELHVAGLMPPSMLGDYALPACDWHWYYCENAWSLQGLQRFADVLAEL
                     DPAAAEVYGREAADFRHDLRAALDREAALSPV"
BASE COUNT          246 a          574 c          472 g          212 t
ORIGIN      
        1 aaccggcgac agcgcggcct cgcgatcgag agcggcccgc agatcgtgtc gaaagtccgc
       61 ggcctcccgg ccatagacct ccgccgccgc gggatccagc tcggcgagca catccgcgaa
      121 ccgctgcagc ccctgcagcg accaagcgtt ctcgcagtag taccagtgcc agtcacaggc
      181 ggggagcgca tagtcgccga gcatcgacgg cggcatgaga cccgctacgt gcagctcgtc
      241 tcggttcgga cagtcctcca tgtagcgatc gcgctcacga atgatccaat ccgcggcggc
      301 ctgcagacgc tcgcggtgcg cggcgaacca gtccctgtcg cccgtcagaa agtatcggtc
      361 gcacatcgcc catagaagcc ggcccgtcga ggcgtgggta ccctcgtggc tgtaacccat
      421 gtcatgccgc atcgacgtcg cccactcgag cgaccccgcc gggtcagtga agtcgccgtc
      481 ggacttcacg cccggcgagc cgaggaagta gtcgtagacc ggcatcacct cgtcgtgaag
      541 accgaccagc tccatcacgc gcgctgctcg cgcgatctcg gcactgagag tcggccacag
      601 atgccggccg cgcaactcgt cgcaggccgt gcgccaggcg ccggtccagc gcgcgtcgga
      661 cagctcgacc gtcatcgccg gatcgggcac ttccgggaag ggcggcaccg gcgtgccctc
      721 gggacaggtc cagaaccgaa cctgctccat caactcctcc cacgatcgcg gctcgcgatg
      781 ctcgcgggtc atctgccgca agctcttcct gccctcagcc tccaaccgcg ccgcgaacgc
      841 agcaccagtc tccgcgctgc ccgcgcgcga gatgaacaac ccctgccgcg ggaggtagat
      901 cggccccgac tccaggtcct tcacgctgaa ggtcacatca cccgcgtcgg tccacagcgt
      961 cacacggctg tcgaggcccg ggcgtatcgt gggggcgtag agcaacgaga cccgcaggcc
     1021 ccggcgttcg ggcgaacggt gctccgccgc tgagcgccaa cgcgccccgc ccatgacggt
     1081 ggtcgtggcg tcgccccgaa gcggcgcgac cgtcccgatg actgccacgt gggtctcgag
     1141 tcggccactg acgggtttcc tctccgtccc cggccggaac ccccattcga tctccaagtc
     1201 catcggcgcc catacacccg tgctcggccc gctgacccgg aggcgcggaa ccggcagctc
     1261 gcccgagccg aacacggcca ccatctcggt cgctgccggg acgcgggtgc tgtaggaggt
     1321 gtccatcagc tccgtcgggt cgctccggta ggtccacctg cgcccgtcct ccgaaaccgt
     1381 cggctcgccg agggccttgt ccacggtcca gccgaaccag ccgaaggcgg tggggtagac
     1441 ccgcacctca acctgccccg gatccgggac cggcgaatcc gctggccact cgagctccac
     1501 cgcg
//
LOCUS       DAOSPL010001346         1502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_84505_length_1502_cov_4.116793, whole genome shotgun sequence.
ACCESSION   DAOSPL010001346 DAOSPL010000000
VERSION     DAOSPL010001346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84505_length_1502_cov_4.116793"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PLQ54_19615"
     CDS             <1..871
                     /locus_tag="PLQ54_19615"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOS95530.1"
                     /translation="MHLDCLVCLGGGGTQKNALHLMRAGQIPVLTLPKTIDNDVAETD
                     VSFGYDTAMTIAAEAIDRLHTTAESHHRLMVVEIMGHRTGWLALGAGVAGGADVILIP
                     EIPYDLEAVAASVVSRNRKGKRFSIVALAEGALSKEDAAAMERARAAAAADKAPKDKA
                     KAGAAKRRTPSRHAAIDVKPAEDALPGTVGARIAARLQEMTGLESRVTVLGHLQRGGT
                     PSPFDRLLATRLGVAAAKLLDAGQFGVMVAARGDQCVPVPLEDVAGKRKLVPLDHPLL
                     ETARLVGTCLGDS"
     gene            861..>1502
                     /locus_tag="PLQ54_19620"
     CDS             861..>1502
                     /locus_tag="PLQ54_19620"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOS95531.1"
                     /translation="MTRSAGECVIGLDVGTQGARAVAVTAQGQVLASASESLRDAVVE
                     LPKPLVEQRPEAWWEAAALCLRQVVVGLGAVHVRAIAVDSTSGTFVPIDAAGRPLRPA
                     IMYNDGRAGTDLADEVQQAGQELASRLGYRFPPAFALPKMLWLRRHEPEVFERAARLI
                     HATDYIVGRLTGRFDVTDTSTALKSGVDLETGAWPSFLWDALGIPRGKLPDVVS"
BASE COUNT          227 a          516 c          536 g          223 t
ORIGIN      
        1 aatgcacctc gactgcctgg tctgtctggg tgggggtggc acgcagaaga acgcgctgca
       61 cctgatgagg gcgggccaga tccccgtgct cacgctcccg aagaccatcg ataacgatgt
      121 ggcagagacc gacgtgagct tcggctatga cacggccatg accatcgccg ccgaggccat
      181 cgaccggttg cacaccacgg cggagagcca ccatcgcctg atggtggtgg agatcatggg
      241 ccatcgcacg ggctggttgg ccctgggcgc gggcgtcgcc ggcggggcgg acgtgatcct
      301 tatccccgag atcccctacg acttggaggc cgtggccgcc agcgtggtga gccgcaatcg
      361 caagggcaag cggtttagta ttgtagcact cgccgagggc gcactcagca aggaggacgc
      421 agcggcaatg gagcgcgccc gggcagccgc cgccgcggac aaggccccga aggacaaggc
      481 gaaggctggt gcggccaaac gtaggactcc gtcgcgccac gcggcaatcg acgtcaagcc
      541 ggccgaggac gccttgcccg gtaccgtggg cgcgcgcatc gccgcccgcc tccaggagat
      601 gacgggcctc gaatcgcgcg tcacggtgct cgggcacctt cagcgcggcg ggaccccctc
      661 acccttcgac cgcttgctgg ccacgcgtct tggtgtcgcc gcagcgaagc tgttggatgc
      721 gggccagttc ggcgtgatgg tggccgcgcg cggcgaccag tgcgtgcccg tcccgctcga
      781 ggatgtggcc gggaagcgga aactcgtccc gctcgaccac cccctgctcg agaccgcccg
      841 gctcgtgggg acgtgcctgg gtgactcgta gcgccgggga gtgcgtgatc ggactcgacg
      901 tcggcaccca gggcgcccgg gccgtggcgg tgacggcaca gggccaggtg ctggcgtcgg
      961 cctccgaatc gctccgggat gccgtggtcg agctgcccaa gccgctggtc gagcagcggc
     1021 ccgaggcgtg gtgggaggcc gcggccctct gcctccgtca ggtggttgtg ggactcggcg
     1081 cagtccacgt gcgggcgatc gccgtcgact cgacatcggg caccttcgtc cccatcgacg
     1141 cggccgggcg ccccctgcgt ccggcgatca tgtacaacga cggccgggca ggaaccgatc
     1201 tggccgatga ggtccagcag gcgggtcagg aactggccag tcggctcggg taccgctttc
     1261 cgcccgcatt cgccttgccc aagatgctct ggctcagacg ccacgaaccg gaggtgttcg
     1321 agcgcgcggc ccggctcatc cacgccaccg actacatcgt cggccggctg acgggccgct
     1381 tcgacgtgac cgacacctcc accgccctca agtcgggtgt cgacctcgag acgggtgcct
     1441 ggccgtcgtt cctctgggac gcgctcggaa tcccgcgcgg caagttgccc gacgtggtct
     1501 cg
//
LOCUS       DAOSPL010001347         1502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_84533_length_1502_cov_2.723566, whole genome shotgun sequence.
ACCESSION   DAOSPL010001347 DAOSPL010000000
VERSION     DAOSPL010001347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84533_length_1502_cov_2.723566"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..829)
                     /locus_tag="PLQ54_19625"
     CDS             complement(134..829)
                     /locus_tag="PLQ54_19625"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOS95532.1"
                     /translation="MPEPLTLPVVHIVADTLPAAWEQAVVETWERGARIPTQYDQDGD
                     PASRDVSLFLAVRDPFAEPRIHRAFPGGIEDLEVYRQEVADGIHDHWIDPAAGKWEYT
                     YHERLRAYGVPGLPEPVDQIAYVVDALAKAPHTRRAQAVLWKCWEDAGIADPACLQRL
                     WFRVYGEELVLAAHMRSNDAFKAAYMNMYAFTDLQRAVAEALSAKLGRQIRVGQYNHV
                     VDSFHIYGSYFGV"
     gene            1248..>1502
                     /locus_tag="PLQ54_19630"
     CDS             1248..>1502
                     /locus_tag="PLQ54_19630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95533.1"
                     /translation="MARLIQVLRRHSGRAVLCIAGLATAAVLGQPARPGPADPRAIAE
                     AVIEGCRAREQAGAGVEFTVLAERFVGAWKLGLATDGDPAA"
BASE COUNT          225 a          506 c          544 g          227 t
ORIGIN      
        1 gcgccccgtc agccgctcct ggtccatcga cttggcgatg cgctcgcgcg cctcgtcgat
       61 gatgggctgc acctgttccg tcgtccagac gcggtcctcg aacggccggg cctgtagggt
      121 gcgcaggaac ccctcaaact ccgaagtagg acccgtagat gtggaacgag tccaccacgt
      181 gattgtactg accgacgcgg atctggcgcc ccagtttcgc cgagagtgcc tccgccacag
      241 cacgctgcag gtcagtgaaa gcgtacatgt tcatatacgc cgctttgaac gcatcgttgc
      301 tgcgcatgtg ggcggcgagc acgagctcct cgccgtagac gcgaaaccag aggcgctgga
      361 gacaggcggg gtccgcgata cccgcatcct cccagcactt ccacagcaca gcctgggcgc
      421 gacgggtgtg cggggccttg gctagtgcat cgacgacgta ggcgatctgg tcgaccggct
      481 cggggagacc cggcaccccg taggccctca atcgctcgtg gtacgtatac tcccacttgc
      541 cggcggcagg atcgatccag tggtcgtgaa tgccgtcggc gacctcctgc cggtagacct
      601 ccaggtcctc gatgccgccg gggaaagcac ggtggatgcg gggctcggcg aatgggtcgc
      661 gaacggcgag gaagaggctg acatcgcggc tggcgggatc accgtcttga tcatactggg
      721 tgggaatgcg cgcgccgcgc tcccaggtct cgaccacggc ctgctcccaa gcggcgggga
      781 gggtatcggc cacgatgtgc acgaccggca gcgtcagggg ttcgggcatc agatcctcct
      841 tctggacggg gcgggtggcg ccgcctccgg cggcagccag cttagcatac ctggggcgtc
      901 gtgggggtac cgcgcgggac ggacgctcgt gggctcggcg gggcgcctca gcagagcccg
      961 gtggtgccgt ccccgccccg ccgtacgcac aggccattcc cggagcgcgg agcgcctgct
     1021 ccgcgagagc agggcacggc aacgatgcgg gctgtgtggt ggggcgaccg cagttggcag
     1081 ccggcgtcct gggctagcac cagtggcccc tcggccctcc gcctggcccg ggttgccgcc
     1141 accccaacaa cgccggcgcc ggtggctgcc acacgtacat gcgggccggt accgcgacgc
     1201 gagtcgtccg gcctgacccg cttcccggaa gcgaggaggg gcgaccgatg gcgcgactga
     1261 tccaagtgct gcgtcggcac agcggccgtg ctgtgctgtg catcgcaggg cttgccacgg
     1321 cggcggtgct gggccagccc gcaagaccgg gcccagccga cccgcgggcg atagccgagg
     1381 ccgtcatcga gggctgccgc gcccgcgagc aggctggtgc gggtgtcgag ttcacggtac
     1441 ttgccgagcg gttcgtcggg gcgtggaagc tcggcctcgc gaccgacgga gaccccgctg
     1501 cg
//
LOCUS       DAOSPL010001348         1500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_84690_length_1500_cov_2.899654, whole genome shotgun sequence.
ACCESSION   DAOSPL010001348 DAOSPL010000000
VERSION     DAOSPL010001348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84690_length_1500_cov_2.899654"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLQ54_19635"
     CDS             complement(<1..296)
                     /locus_tag="PLQ54_19635"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOS95534.1"
                     /translation="MGTCRSLLLPAAVAALLILHGCGGTNQTINPFPEVAPPGTVVRS
                     AKARNTAPVVSAADMAALAAGNREFACALYQRLARGDGNLVFSPLSISTALAMA"
     gene            414..1118
                     /gene="sfsA"
                     /locus_tag="PLQ54_19640"
     CDS             414..1118
                     /gene="sfsA"
                     /locus_tag="PLQ54_19640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HOS95535.1"
                     /translation="MKHPHPLVPAQFDRRCNRFAALVRLDGQSEYVHVPNSGRMWELL
                     VPGVPVLLTPTPGADRRTRYCLSLVWHRRRWVGVDSRTPPSLLREAIEAGRLSNWASS
                     RVVASEPAFGHGRLDLVVERRGRPVLVETKSVNCVVDGVALFPDAPTERGVRHLVEMA
                     EAARGGQELAVWFVVQRSDARLCAPYWERDPALGEALLEAARSGVEVSACRCRVGAAG
                     IAITHEIPVRLQRPAE"
     gene            complement(1100..>1500)
                     /locus_tag="PLQ54_19645"
     CDS             complement(1100..>1500)
                     /locus_tag="PLQ54_19645"
                     /inference="COORDINATES: protein motif:HMM:NF005840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOS95536.1"
                     /translation="RNAVLPRTTASIVCAIRDFWVAEVDGRVAGCCALTVIDEDLVEV
                     RTLVVDEAIQGLGLGRKLVEACIAEAESLGFSTVFALTRVVGFFERMGFTPVPMSSLP
                     QKVWLDCVHCPSFPNCDEVAMARPLHSAGR"
BASE COUNT          230 a          491 c          547 g          232 t
ORIGIN      
        1 tatgccatcg ccagcgcggt cgagatgctc agcggcgaga acacaaggtt gccgtcgccg
       61 cgggccagcc gctgatagag ggcacaggcg aactcccggt tccccgctgc cagcgccgcc
      121 atgtccgccg cggagaccac gggcgccgta ttgcgcgcct tggccgagcg cacgacagtg
      181 ccgggagggg ccacctcggg gaacgggttg atggtctggt tcgtgccacc acagccatgg
      241 aggatgagca gtgcggccac ggcggccgga agcagcaggg aacggcatgt gcccatcgtc
      301 gagccctcca gcgggggcag aggcccgcgc ccccccgatt ctagactcct ctggcgccat
      361 cgacggctcc ggattccgca gcgtggcccc acctatggca tcatggaggg accgtgaagc
      421 acccacatcc cctcgttcca gcgcagttcg accggcgatg caaccggttt gcagcgttgg
      481 ttcgccttga cggacaatct gaatacgttc atgttcctaa ctcaggccgg atgtgggagc
      541 tgctggtgcc gggcgttccg gtgttgctga cgcccacgcc tggcgccgat cggcggacgc
      601 ggtactgcct gtcgctggtg tggcaccgca gacgctgggt gggcgtggac tcccggacgc
      661 caccaagtct gctacgcgag gccattgaag cggggcgcct ctccaattgg gcctcctcgc
      721 gtgtcgtggc gtccgagccg gcgttcggcc atgggcggct ggacctggtg gtggagaggc
      781 gtggccgccc ggtgctggtc gagaccaagt cggtgaactg tgtggtcgac ggcgtggcgc
      841 tgttccccga cgcccccaca gagcgaggcg tccgccacct cgtcgagatg gcggaggcag
      901 cccggggcgg ccaggaactg gccgtctggt tcgtggtgca gcgctccgat gcgcggctgt
      961 gcgcgcccta ctgggagcgc gacccggcac tgggcgaggc gctcctcgag gcggcccgga
     1021 gcggcgtcga ggtgagcgcc tgccgttgcc gagtgggtgc ggccggcatt gccatcaccc
     1081 acgagatccc ggtccggctt cagcgtccgg ccgagtgaag cggacgagcc atggcgacct
     1141 cgtcgcagtt ggggaagctc gggcagtgta cacagtcgag ccagaccttc tggggtaggg
     1201 agctcatggg gaccggggtg aaccccatgc gctcgaagaa gcccacgacg cgtgtgagcg
     1261 caaacacggt gctgaagccc aacgattcgg cttcggcgat gcaggcctcg acgagcttgc
     1321 ggccgagccc cagcccctgg atggcttcgt cgacgaccag agtgcgcacc tcgaccaggt
     1381 cctcgtcgat gacggtgagg gcgcagcagc cggccacgcg gccgtcgacc tcggcgaccc
     1441 agaagtcgcg gatggcgcac acgatgctgg ccgtggttcg aggcaggacg gcgtttcggg
//
LOCUS       DAOSPL010001349         1496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_84976_length_1496_cov_4.676613, whole genome shotgun sequence.
ACCESSION   DAOSPL010001349 DAOSPL010000000
VERSION     DAOSPL010001349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84976_length_1496_cov_4.676613"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PLQ54_19650"
     CDS             <1..543
                     /locus_tag="PLQ54_19650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95537.1"
                     /translation="EELGSVTGASDAAESRFYSARYEYGQGWRGGYLDRPGKDQTLEA
                     LFTDRVVIFNGHGSETTIRNNKEPDKVTAASIRGLRNQYGFTSQAKLVVLQCCKAAMD
                     TPTSVANALAYVGVGCVIGWEVISYTYDPGFLELMDLMCNYGLTAQQAYEQVCYDHSD
                     RWGPGKTRIIGDTSLWGEPL"
     gene            540..>1496
                     /locus_tag="PLQ54_19655"
     CDS             540..>1496
                     /locus_tag="PLQ54_19655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95538.1"
                     /translation="MRRRELCLALATVVSGPWSDHAWSALTDDEAKEVAMRVVERVAG
                     TAEQAAVRYRGRRGPTVVVDERWWVWVDSAMARAAKLSDQGYRLSAHAKHKLVAPPAH
                     AEIEAAALELLHRVFPGFDMAEFVVESAKPQGEQARVEARWRRVIPECGFPGPGLCFV
                     DFCWPDRAISGMGAECPPIPDEWRRPASIAAEQAEATARDALGDLRGGWLRTTKDYCV
                     VDPRDDAKSRPAWNVTLLLGSRDKPEECEADRSVFVDPWSGDVVTIKECMGDRPVGTS
                     GPVVASRGASRAGARTVGAVAAHSWPPWAGGGAAVVVIALGAT"
BASE COUNT          253 a          456 c          546 g          241 t
ORIGIN      
        1 gaggaactgg ggtcagtgac cggtgcgtcg gacgcagccg agtcgcgctt ctactcggct
       61 cggtatgagt acggacaggg gtggcgcggc gggtacttgg accgccccgg caaagaccag
      121 acgctcgagg cgctgttcac ggacagagta gtcatcttca acggtcacgg gagcgagacg
      181 accatccgca acaacaagga gcctgacaag gtgactgccg cctcgatccg ggggctccgc
      241 aaccagtacg ggttcacttc gcaggccaag ctcgttgtcc tgcagtgctg caaggcggcc
      301 atggacacgc cgaccagcgt cgccaatgcc ctcgcgtacg tcggcgtcgg ctgtgtcatc
      361 gggtgggagg tcatctccta cacgtacgac ccgggcttcc tggagctcat ggatctgatg
      421 tgcaactatg ggctgaccgc gcagcaagcc tacgagcagg tgtgctacga ccactcagac
      481 cggtggggtc ccggcaagac ccggatcatc ggggatacat ccctgtgggg ggagccgcta
      541 tgaggagacg cgaactctgt ctggcgctcg cgacggttgt gagtgggcca tggagcgacc
      601 acgcctggtc cgccctcacc gatgatgagg ccaaagaagt ggcgatgcgg gtggtggaac
      661 gggtcgcagg cactgccgag caggccgcag tgaggtaccg tggccggcgg gggcccaccg
      721 tcgttgtcga cgagcggtgg tgggtgtggg tcgactcggc gatggcgcga gccgccaaac
      781 tgtcagatca ggggtaccgc ctgtcggccc acgcgaagca caagctggtt gcgccccccg
      841 cccacgccga gatcgaggcc gcagctctcg agctactcca cagagtcttc ccgggattcg
      901 acatggccga gttcgtggtc gaatcggcca agccgcaggg cgagcaggcc cgcgtggagg
      961 cgcggtggcg gcgcgtgatc cccgagtgtg gtttccccgg ccctgggctg tgctttgtgg
     1021 acttctgttg gcctgaccgg gcgatctcag ggatgggtgc tgagtgcccg cccattcccg
     1081 acgagtggcg caggccggcg tccattgcag ccgagcaagc ggaggccaca gcgagggatg
     1141 ccctcggaga ccttcgaggc ggctggctgc gaacgaccaa ggactactgt gtcgtcgatc
     1201 ccagggacga cgcgaagtcg cggccggcct ggaacgtgac actcctcctc gggtcccgtg
     1261 acaagcccga ggagtgcgag gccgatcgga gtgtcttcgt tgacccatgg agcggcgatg
     1321 tggtcacgat caaggagtgc atgggcgaca gacccgtcgg gacatcaggg ccggtagtgg
     1381 catcgagggg cgcctcgcgg gctggtgcgc gaaccgtcgg cgccgttgca gctcactcct
     1441 ggcccccctg ggccggcggg ggggctgcgg tggtggtgat cgcactcggc gcaaca
//
LOCUS       DAOSPL010001350         1496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_85015_length_1496_cov_2.104094, whole genome shotgun sequence.
ACCESSION   DAOSPL010001350 DAOSPL010000000
VERSION     DAOSPL010001350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85015_length_1496_cov_2.104094"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLQ54_19660"
     CDS             complement(<1..242)
                     /locus_tag="PLQ54_19660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95539.1"
                     /translation="MRALLLVTALCTPAALEGENLLRNGDFESGLEGWALLHPPANAV
                     TIEPGPSGNCACLSATDRSGSTYITQALDPERIRGRT"
     gene            382..1374
                     /locus_tag="PLQ54_19665"
     CDS             382..1374
                     /locus_tag="PLQ54_19665"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOS95540.1"
                     /translation="MKSLRFAGYSKVEIEDVPEPRPTADQVVIDIKVSALCGSEMGVF
                     RSPEGRHGNPGHEFVGEVVDPNGHPEFAVGDRVGAHVIVGCGTCPHCQEGNHVFCPNM
                     LPSGYAHAEYIALSARHCLRVPDGMDWDTGVMVAGDAMGVAYHLTRRVGLQAHDTVAV
                     FGCGPVGLGAVLLWSFLGARVIGVDMSAYRRDLALKLGAWRTVAAGKHGTVDRLRKLT
                     GGAGPDTCLECSGNPLAIEAAFDAVRKGGKVGIVGEQPLASFSPSGHLIHKELSVFGA
                     WYFRLSEFAAMVELCSRGLKPRRVITHRFPLEEAQKGYDLMARQECGKVLFVQY"
BASE COUNT          231 a          509 c          522 g          234 t
ORIGIN      
        1 gtccgtcccc ggattcgctc aggatcaagc gcctgtgtga tgtacgtgga gccactgcgg
       61 tcggtggcgc tgaggcaggc gcagttgccc gacgggcctg gctcgatggt gacggcgttc
      121 gctggagggt gtagcagggc ccagccctcc aggccggact cgaagtcgcc gttgcgcaac
      181 aggttctcac cctcgagtgc agcgggggtg cagagggcgg tcacaaggag tagagcgcgc
      241 atggggacct cctaggggct aggcacggtt gccgccttta gcttctccgc cgtcctctgc
      301 cggcctgcgc ggcaggcccg cgcggcagcg cggagaacga catactccac gtcccaccac
      361 gccggcagcc ggaggcgagc catgaaatcg ctgcgattcg cgggctactc gaaggtcgag
      421 atcgaggatg tgcctgagcc ccggccgaca gccgaccagg tcgtcatcga catcaaggtc
      481 tccgcgctct gcgggagcga gatgggcgtc ttccggagcc ccgagggccg gcacggcaac
      541 cccggacacg agttcgtggg cgaagtcgtg gatcccaacg gccaccccga gttcgccgtg
      601 ggcgaccgcg tgggcgcgca cgtcatcgtg ggctgcggca cttgcccaca ctgccaagag
      661 ggcaaccacg tgttctgtcc caacatgctg cccagcggat acgcccatgc cgaatacatc
      721 gcgctgtccg cacggcactg ccttcgagtc cccgacggca tggactggga caccggggtg
      781 atggtcgccg gcgatgccat gggcgtggcc taccacctga cgcggcgagt cggtctgcag
      841 gcgcatgaca cagtggcggt cttcggctgc ggccccgtcg gtctgggggc cgtgctgctg
      901 tggagcttcc tgggcgcccg cgtcatcggc gtcgatatga gcgcctaccg ccgcgacctg
      961 gccctcaagc tcggcgcctg gcggaccgtc gccgccggga agcacggtac cgtcgaccgg
     1021 ctgaggaagc tcaccggggg cgccgggccc gacacctgcc tcgagtgctc cggcaacccc
     1081 ctggccatcg aagcggcatt cgatgcggtc cgtaagggcg gtaaggtggg gatcgtgggt
     1141 gagcagccct tggcctcgtt cagccccagc ggtcacctga tccacaagga actgagcgtc
     1201 ttcggagcct ggtacttccg cctgagcgag ttcgccgcca tggtggagct ctgcagccgc
     1261 gggctgaagc cccgcagggt catcacccac cggttccctc tcgaggaagc gcagaagggc
     1321 tacgacctca tggcgcggca ggaatgcggc aaggtgttgt tcgtgcagta ctagccgacc
     1381 cgcagtgccc ggccccacgt gccgcgctgg cactccaggc cactccaccc gtgagtgcag
     1441 aggcctcggc ccgccgcgct agagcccctt cagcacgtcg gcggggctct ctggga
//
LOCUS       DAOSPL010001351         1495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_85088_length_1495_cov_2.363194, whole genome shotgun sequence.
ACCESSION   DAOSPL010001351 DAOSPL010000000
VERSION     DAOSPL010001351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85088_length_1495_cov_2.363194"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1495)
                     /locus_tag="PLQ54_19670"
     CDS             complement(<1..>1495)
                     /locus_tag="PLQ54_19670"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS95541.1"
                     /translation="GNLAALGTRITEAAAGVLDVERVSLWRISEDGESLSCVDLFDRR
                     PGEHSVAAPMQRAEFGSVFAALEELPYVAADDAMSDPRTAGFVGRFLEPQDIRSMLIA
                     TVRASGRYLGALCFSQVGRQRHWERDEEALACQLADQVALAVLSRDRWAAEQSARKMA
                     AAAEAANAAKSEFLANMSHEIRTPMNAVIGMTELLLDTDMNAEQREYASTIRSSGEAL
                     LNIINDVLDFSKMEAGHLSIEATPFGLRTAVEDAVKSLALKAHEKGLELVCRIEHDVP
                     EMLLGDPGRLRQVIVNLIGNAIKFTATGEVAFRVAVESEGEDRVTLHFSVADTGIGIA
                     RSKLESIFEAFTQADSSTTRRFGGTGLGLAICRRIAELMGGRIWAESEEGRGSVFHFV
                     APFELRQAGPAAREDGPLAGLRVLVVDDNETNRAMLRECLTAWGLAVDDEPDGPSALA
                     RLEGPDEEAGPYAVILLDRAMPDMDGFTVAERILSNPRLAVTPILMLT"
BASE COUNT          243 a          541 c          473 g          238 t
ORIGIN      
        1 aggtcagcat caggatcggc gtgaccgcga ggcgaggatt cgagaggatc ctctcggcca
       61 ccgtgaaccc gtccatgtcc ggcatggcgc ggtcgagcaa gatcaccgcg tagggtccgg
      121 cctcctcgtc tggaccctcc aagcgcgcca gggcggacgg cccgtcaggc tcatcatcca
      181 ccgccaggcc ccatgcagtg agacactcgc gcagcatggc ccggtttgtc tcattgtcgt
      241 cgacgaccag cacgcgcagc ccggcaagcg gaccgtcctc cctcgcggcg ggaccggcct
      301 gcctgagctc gaatggggcc acgaagtgga agacgctgcc ccggccctcc tcgctctcgg
      361 cccagatcct cccgcccatg agctccgcga tgcgacggca gattgccagt ccaagccctg
      421 tgccgccgaa ccggcgtgtg gtagagctat cggcctgcgt gaacgcttcg aagatcgact
      481 cgagcttgga ccgcgcgatc ccgatgcccg tgtcggccac cgagaagtgc aacgtcacgc
      541 gatcctcccc ctcggactcg acggctacgc ggaaggccac ctcgcccgtg gccgtgaact
      601 tgatggcgtt gccgatgagg ttcacgatga cctgccgcaa gcgccccgga tctcccagca
      661 gcatctcggg cacgtcgtgt tcgatccggc acaccagctc gagccccttc tcgtgggcct
      721 tcagcgccag gctcttgacc gcgtcctcca cggccgtccg caaaccgaag ggcgtggcct
      781 cgatggacag atgcccggct tccatcttcg agaagtcgag cacatcgttg atgatgttca
      841 gaagtgcctc gcccgatgac cggatcgtcg atgcgtactc ccgctgctcg gcgttcatgt
      901 ccgtgtcgag caggagttcc gtcatgccga tgacggcgtt catcggcgtg cggatctcgt
      961 gcgacatgtt ggccaggaac tcgctcttgg cggcattggc cgcctcggcg gccgcggcca
     1021 tcttccgggc cgattgctcc gccgcccacc ggtcgcggct gagcacggcc agagcgacct
     1081 ggtcggccaa ctggcaggcc agcgcctcct cgtctcgctc ccagtggcgt tgccgcccga
     1141 cctgagagaa gcatagggcc cccaggtaac ggcccgaggc gcgcacggtg gcgatcagca
     1201 tcgaccggat gtcctgcggc tccaggaagc ggcccacgaa gccggcggtg cgcgggtcgg
     1261 acatggcgtc atccgccgcg acgtatggga gctcctcgag cgccgcaaag accgagccga
     1321 actcggcccg ctgcatgggc gcagccaccg agtgctctcc cgggcgccgg tcgaacaggt
     1381 ccacgcacga gagcgactca ccgtcctcgc taatccgcca caggctgact cgctcgacat
     1441 cgagaacacc cgcggcagcc tcggtgatcc gcgtgcccag agccgccagg ttgcc
//
LOCUS       DAOSPL010001352         1493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_85213_length_1493_cov_3.200278, whole genome shotgun sequence.
ACCESSION   DAOSPL010001352 DAOSPL010000000
VERSION     DAOSPL010001352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85213_length_1493_cov_3.200278"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLQ54_19675"
     CDS             <1..452
                     /locus_tag="PLQ54_19675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOS95542.1"
                     /translation="LIEVMRATGAQVLVNEARCIERDGDQIWVAGVDDPSRDRHDLAR
                     ALAQTRGDHFRLLLAHSVDIRKAAAAEAVDFVVCGHSHGGQMCLPWIGPLVSGAKQPS
                     SPRLARGVHRFGTTWAYVCRGTGTSKIPIRLWCRPEVTRYRFVRRPA"
     gene            complement(449..646)
                     /locus_tag="PLQ54_19680"
     CDS             complement(449..646)
                     /locus_tag="PLQ54_19680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95543.1"
                     /translation="MAIPKQCCTCLRVWEPGSGTSGAWLPARTKLRDATHTYCPSCMD
                     QLRRSFGAFHAALGKAAKPVP"
     gene            complement(916..>1493)
                     /locus_tag="PLQ54_19685"
     CDS             complement(916..>1493)
                     /locus_tag="PLQ54_19685"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOS95544.1"
                     /translation="ASAIALLAATATQWRSAMAPSAVATVVAAQGVGQVLRASGAEAA
                     VPGARIEVGDTIATQPGGSVCLCFPDRSCVTVQGGTMVRIVELPPTRSPARLDLERGL
                     ITGRVQHGREMLVRTPCATASAEGTRFCLNATDEQSCLTVREGRVGLANDHGQVHATA
                     MQHASATRTAAPEAPVSAPIPDSGGHCSRSF"
BASE COUNT          229 a          516 c          519 g          229 t
ORIGIN      
        1 cgctcatcga agtcatgcgc gccacgggtg cccaggtgtt ggtcaatgag gcccgctgca
       61 tcgagaggga cggtgaccag atctgggtcg cgggtgtaga cgatccgtcc cgcgatcggc
      121 atgacctggc ccgggcgttg gcccagaccc ggggcgacca tttccgcctt ctgcttgccc
      181 acagcgtcga cattcgcaag gcagcggccg cggaggcagt ggattttgtc gtctgtggcc
      241 actcgcacgg tgggcagatg tgcctgccat ggatcgggcc actcgtgtca ggggccaagc
      301 agcccagcag cccacggctg gcgcgcggag tccaccggtt cggcaccacc tgggcctacg
      361 tctgccgggg gacaggcacg agcaagatcc ctatccggct atggtgccgg cccgaggtga
      421 ctcgctatcg cttcgtccgg cgtccggctt aggggacggg cttggccgcc ttgcccaagg
      481 ccgcgtgaaa ggcgccgaag gaccggcgga gctggtccat gcacgagggg cagtaggtat
      541 gtgtcgcgtc gcggagcttc gtccgcgcgg ggagccacgc gcccgacgta ccactgccgg
      601 gctcccagac tctcaggcat gtgcagcact gcttgggtat ggccactggg catcgacttc
      661 cctgactggc gggacccaca actactcgat gggcgcagct accagccccg ttaccctact
      721 gcttgggccg tggtccggtt caccacgtca tgctcaggcg gacgcgactg cccggcctcg
      781 cggggctttc ttgtctacct catcggcaga gcgggcccgt ggccttagcg gcgccaccgg
      841 gtcgggggac agccagaaac gaagtcagcg cccggattgg ctcgggcgct gacgagctct
      901 cggcgaggcc ggaggctaga aggaccgcga gcagtgaccg cccgagtcgg ggatcggcgc
      961 actgacaggc gcctcaggcg ccgccgttcg cgtcgcgctg gcgtgctgca tggccgtcgc
     1021 atgcacctgg ccgtggtcat tggccagtcc cacccggccc tctcggacgg tgagacacga
     1081 ctgctcatcc gtcgcgttga ggcagaagcg cgtgccttcg gccgaagccg tcgcgcaggg
     1141 cgtccgtacc agcatctccc ggccgtgctg aacacggccg gtaatgaggc cgcgctccaa
     1201 gtccagccgg gccggagacc gggtcggggg aagctcaacg atgcggacca tggtaccgcc
     1261 ctgcaccgtg acgcacgatc ggtcggggaa gcacaggcac accgatcctc ccggctgcgt
     1321 tgcgatggtg tcaccgacct cgatccgtgc gcccgggacc gcggcctcag cccccgacgc
     1381 ccgcagcacc tgcccgacgc cctgggcggc cacaaccgtg gcgacagccg acggcgccat
     1441 ggctgatcgc cactgagtcg ccgtggcagc cagaagcgca atggcgctcg ccg
//
LOCUS       DAOSPL010001353         1489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_85529_length_1489_cov_3.049512, whole genome shotgun sequence.
ACCESSION   DAOSPL010001353 DAOSPL010000000
VERSION     DAOSPL010001353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85529_length_1489_cov_3.049512"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1069
                     /locus_tag="PLQ54_19690"
     CDS             164..1069
                     /locus_tag="PLQ54_19690"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOS95545.1"
                     /translation="MRWGLALLSMVLPWVGAGDDLPEGLVRDLAKAAHMPPDPSYLPG
                     GWVVNAKDGSILVPIAGGRAIFGAAGEEAFDDARPRFLMEMGPFYLALHPVTHTQYAR
                     FLTERAATPEQVAEWIDLSHPQCGIERMGATYRARPGVAQVPVLAVSWMGAQAYCEWA
                     GLRLPRELEWERGARGTDGRLYPWGDEWGPARCRNFTLPDEQEFGPCGIWQYPAGRSP
                     DGLFDMSGNVPEWCADWYEEDAYGRYSHEDPSPPARGEWRTMRGGGWGDRDSVMFSAW
                     CRGMGEPGSMWDRNSGYGFRCARDM"
     gene            1133..>1489
                     /locus_tag="PLQ54_19695"
     CDS             1133..>1489
                     /locus_tag="PLQ54_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95546.1"
                     /translation="MRWLLSLSVALPWLAAPALAQADGNLVANGGFEADADGDGLADG
                     WTAPHDEGVEVTTSLDAGATGEHSQKLACTRFERTSPASHAMLAQVGYIAVAEGQWYR
                     LRFRARQQGIPGGAIDV"
BASE COUNT          229 a          427 c          575 g          258 t
ORIGIN      
        1 catcaccatg cgcgtccacg accagacctg ggccgggggg atctggaagc cggtctggat
       61 cgtggcgagt gagtagggga gggcggcgcg tccgccgcca caggttgtcc agttgtatat
      121 atgtctagac aactggacaa cgtcctgagg gaggtcttgc ggagtgcggt ggggcttggc
      181 tctgctttcg atggtgctgc cgtgggtcgg tgccggtgat gatctgcctg aggggctcgt
      241 gcgcgacctg gcgaaggcgg cgcacatgcc gcccgacccg tcctacctac ccggtggttg
      301 ggtggtgaat gccaaggatg gcagcattct ggtgcccatc gctggggggc gcgcgatctt
      361 cggggcggcg ggcgaggaag ccttcgacga cgcccggccg aggttcctga tggagatggg
      421 tccgttttac ctcgcccttc atccggtaac tcacacacaa tatgcgcggt ttctgacgga
      481 acgcgcggcg acgccggaac aggtcgccga gtggatcgac ctgtcacacc cgcagtgcgg
      541 gatcgagcgc atgggagcca cttaccgcgc gagacccggg gtggcccagg ttcccgtgtt
      601 ggcggtctcg tggatgggcg cgcaggccta ctgcgagtgg gcaggccttc ggttaccgcg
      661 ggagctggaa tgggagcggg gcgcgcgggg gaccgacggg cggctctacc cgtggggcga
      721 tgagtggggt ccggcccgat gtcgcaactt cacgctgccg gatgagcagg agttcggccc
      781 gtgcggaatc tggcaatatc ccgcggggcg gagcccggac gggctgttcg acatgtcggg
      841 caacgtgccg gagtggtgtg cggactggta tgaggaagac gcgtatggcc ggtattcgca
      901 cgaggaccca tcgccgccgg cgcgcggcga atggcggacg atgcgcggcg gtggttgggg
      961 cgatcgggac tcggtgatgt tctcggcgtg gtgccggggg atgggtgagc caggctcgat
     1021 gtgggaccgg aacagcgggt atgggtttcg gtgtgcccgt gacatgtgac gcaagttacg
     1081 tcggataatc catgttatgt tcaatacgcg atttgagcac gaggtgagcc ccatgcgttg
     1141 gctgctgtcg ctctctgtgg cactcccctg gcttgccgcc ccggccctcg cccaagccga
     1201 cgggaacctc gtcgccaacg gcgggttcga ggccgacgct gatggtgacg ggttggccga
     1261 cgggtggacc gcgccgcacg acgagggtgt ggaggtcacg acgtcgctcg acgccggagc
     1321 cacgggcgaa cacagccaga agctggcatg cacgcggttc gagcgaacct cgccggcgag
     1381 ccacgccatg ctggcccagg tgggatacat cgcggtggcc gagggccagt ggtaccggct
     1441 gaggttcaga gcccgccagc agggcatccc cggtggcgcc atcgacgtg
//
LOCUS       DAOSPL010001354         1489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_85551_length_1489_cov_2.417713, whole genome shotgun sequence.
ACCESSION   DAOSPL010001354 DAOSPL010000000
VERSION     DAOSPL010001354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85551_length_1489_cov_2.417713"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1429
                     /locus_tag="PLQ54_19700"
     CDS             89..1429
                     /locus_tag="PLQ54_19700"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOS95547.1"
                     /translation="MLGRWLLLCVFESAFALPIAPAQEPLSEGVDLREFPLAQPIPEK
                     GGLFVHDLDGDGQLDFVVTSPGHIGAYDQSGTPKWVYRGEVFFWPFSAHPCAIAGDLD
                     GDGEQEVAYLSGPQRVAILDAETGEGDGEVQLPKPIQAMAIADLRGLGDRDILLQYDQ
                     THIAAIRADTGELLWETDEYRGIEHSPFRAADLDGDGRDEVAGASLIDDDGHRMNAWD
                     LGGAYLNMDSFVIADVIPGYPLEIVLAEQRGANSHTDVVNANEIVYRSLNPWNWEDPD
                     KVTAGDFDPDRPGLEVFNRSSGGDGTAPRGDEEPFVNEEAPWVLDASGETIAKYYIND
                     HKPEWWTGHGIEGVSRIDWDGDAADELVAKERHRNGAGAIVAPLTGRFVEVFPGKAML
                     IYAVDLAGDSREEVVTLDEGGTIRIHSNARPNPHPERPHPRSQQHYRRQKQNWN"
BASE COUNT          263 a          474 c          511 g          241 t
ORIGIN      
        1 ggtcgggcac cgcgtgaccc ggctactcat cacgtgtcca atgccgcgag attgtggtgt
       61 gaaccgctga acccgcgagg tgtcgccgat gctcggccgg tggctgcttc tctgtgtctt
      121 cgagagcgca ttcgcgctcc ccatcgcccc cgcgcaggag cccctatccg agggcgtcga
      181 tctgcgcgag ttccctttgg cccagcccat ccccgagaag ggtggcctgt tcgtgcacga
      241 cctcgacggc gatggccaac tcgacttcgt ggtcacgagc ccgggccata tcggggccta
      301 cgaccagtcg ggaacgccga agtgggtgta tcgcggagag gtgttcttct ggcccttctc
      361 ggcgcacccg tgcgccattg ccggtgatct ggatggtgat ggcgagcagg aggtcgcgta
      421 cctctcgggt ccgcagcggg tagcgatcct cgatgccgag acgggcgaag gcgacggcga
      481 ggtgcaactt cccaagccga ttcaggccat ggccatcgcc gacctgcggg ggctggggga
      541 ccgtgacatc ttgctgcagt acgaccagac gcacatcgcg gcgatccggg cggacaccgg
      601 cgagttgctc tgggagacgg acgagtaccg cggcatcgag cacagtccgt ttcgggcggc
      661 cgacctcgac ggcgatggca gggacgaggt ggcgggggcg agtctcatcg acgatgacgg
      721 ccaccggatg aacgcctggg acctgggtgg agcctacctc aacatggaca gcttcgtcat
      781 cgccgatgtg atcccgggct acccgctgga gatcgtactt gccgagcagc gcggggcgaa
      841 ctcgcacacc gatgttgtca acgcgaacga gattgtctac cgcagcctca acccgtggaa
      901 ctgggaggac ccggacaagg tcacggcggg ggactttgac cccgatcggc ccggcctcga
      961 ggtcttcaac cgctcctcgg gcggcgacgg cacggcgccc cggggggacg aggagccgtt
     1021 cgtgaacgag gaggccccct gggtcctcga cgccagcggt gagacgatcg ccaagtacta
     1081 catcaatgac cataagccgg agtggtggac cggccacgga atcgagggcg tgtcgcgcat
     1141 cgactgggat ggtgacgcgg ccgacgagct ggtggccaaa gagcgtcaca ggaacggtgc
     1201 cggcgccatc gtcgctccac tcactggccg attcgtcgag gtgttccccg gcaaggccat
     1261 gctcatctac gccgtcgacc tcgccggcga ctcgcgcgag gaggtcgtga ccctcgacga
     1321 gggcgggacg atccgcatcc actcgaacgc gcgccccaac cctcacccgg agcggccgca
     1381 cccgcggagc cagcagcact accgccggca gaagcagaat tggaactaat gacatttccg
     1441 tccagggatt gaaggtacgg aatgggtttg tctggtacat gtgtgcatg
//
LOCUS       DAOSPL010001355         1488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_85604_length_1488_cov_3.697837, whole genome shotgun sequence.
ACCESSION   DAOSPL010001355 DAOSPL010000000
VERSION     DAOSPL010001355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85604_length_1488_cov_3.697837"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            367..1326
                     /locus_tag="PLQ54_19705"
     CDS             367..1326
                     /locus_tag="PLQ54_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOS95548.1"
                     /translation="MALETRYVPKLGRSVTRLGLGALPMGPLQRGLTVEEGARVVRAA
                     VEGGVSFIDTATAYGTYEHVARGLFGWQGTVTVATKTHARADGAAAREHIEMARRELG
                     RDVIDIMLCHCARSLLTREEWGPTLDALLEAKAKGRISLVGLSSHAVEGVRIAARHPD
                     IDVVHPLINLTGMGIVDGTVDEMIAATAEAHDAGKFVYGMKALAGGNLVPRREEALQY
                     AFGLDVLDTIAVGMVTPDEVEWNLRFAQSMAIPKELSERTAAHSKRLNILTFVCQGCG
                     KCVDACENAALSVVEGKAVVDVEQCILCGYCAPHCPMFAIRMV"
BASE COUNT          244 a          500 c          523 g          221 t
ORIGIN      
        1 ccaaggaccg cgcagccagg tcctgacgga cgccatcttg tccgaggccc tgggcctgcc
       61 cctcaggctc gagcagtccg gtaaccgcta ctggccgcgc gtgcggtagg ggcccatgcc
      121 catgtgcgaa acagggaccg gctggggatg gcgctcgcgc cggctctccg gcgccgtgcg
      181 ctcagcgctg ggcgctctgg cccgaacccc aacgttggct acaatggccg gggggagtgc
      241 cccacgcaag gcggaaggca cgcctactga ctccacgggc ccggcccgca ccgtcggacc
      301 ccaagtgcgt gtccatccgt ggctgtctcc ggcacgcatg ccgcgtcacc cgtcttggag
      361 gacccgatgg ctctggaaac acggtacgtc ccaaagctcg gccggtccgt cactcggctc
      421 ggcctgggcg ctctacccat gggcccgctg cagcgcgggc tgacggtcga ggagggggct
      481 cgagtcgtgc gcgcggccgt cgaaggcggc gtcagcttca tcgacaccgc cacggcctac
      541 ggtacctacg agcacgtagc ccgggggctg ttcggctggc agggcacggt caccgtggcc
      601 accaagacgc acgcccgggc cgacggcgcg gccgcgcggg agcacatcga gatggcccgg
      661 cgggagctcg ggcgggacgt cattgacatc atgctctgcc actgcgcgcg cagcctgctc
      721 acccgcgagg aatgggggcc gacgctcgat gcgctgctcg aggcgaaggc gaagggccga
      781 atcagcctcg tcgggctgag ctcgcacgca gtggagggcg tccggatcgc cgcccggcac
      841 ccggacatcg acgtggtcca cccgctcatc aacctgacgg gcatgggcat cgtcgacggg
      901 accgtcgacg agatgatcgc ggccacggcc gaggcgcacg acgcgggcaa gttcgtctac
      961 ggcatgaaag ccctggccgg tgggaatctg gtcccccggc gtgaggaggc cctccagtac
     1021 gccttcggcc tcgacgtact cgataccatc gcggtcggca tggtgacacc cgatgaggtc
     1081 gagtggaacc tgcggttcgc gcagtcgatg gccatcccga aggagctaag tgagcggacg
     1141 gcggcgcact cgaagcggct gaacatcctc accttcgtct gccagggctg cgggaagtgc
     1201 gtcgacgcgt gcgagaacgc cgccctcagc gtcgttgagg ggaaagccgt ggtcgatgtg
     1261 gagcagtgca tcctgtgcgg gtactgcgcg ccgcactgcc cgatgttcgc gatcaggatg
     1321 gtgtaggact accgacgcga gccggaaacc ggtgactgtc acgaatggtc cgcacttaag
     1381 cccaatgcca gaggcgtatg tcgcatcacc tctgtagccg gggctgtagc cgaggcttta
     1441 gcctcggctc acaatggcct gtagagccac ttcgcgcgag gctgaagc
//
LOCUS       DAOSPL010001356         1485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_85864_length_1485_cov_3.099301, whole genome shotgun sequence.
ACCESSION   DAOSPL010001356 DAOSPL010000000
VERSION     DAOSPL010001356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85864_length_1485_cov_3.099301"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PLQ54_19710"
     CDS             <1..303
                     /locus_tag="PLQ54_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95549.1"
                     /translation="VVGAFVRALADEVHYASTDPEALAALNVELEALTRKRLPEAILK
                     PAMERVRFSTIPDPEALQAMADLMHRVGYAKEAIDITGLVDLSVTQPAAPEERAPK"
     gene            300..1082
                     /locus_tag="PLQ54_19715"
     CDS             300..1082
                     /locus_tag="PLQ54_19715"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS95550.1"
                     /translation="MISLTSVSRTYTDSAGARATAAICDVDLVVDAGQFVCIVGPSGC
                     GKSTLLELIAGLQKPDSGEVRVSATGQSRPVLMLQEAALFPWLSVTGNVEFGLAVRGV
                     RRAERREAALRQLAAVRLSRFAHAMPHQLSGGMKQRTALARALAIDPPVLLMDEPFAS
                     LDAQTRDLMHGELQRIWADTGKTIVFVTHNVREAVCLGDRVLLMSARPGRIVDGIDVD
                     LPRPRSVTDPAVSHLAQIALDHLRDEIEKVEREEFDNGTSGP"
     gene            1063..>1485
                     /locus_tag="PLQ54_19720"
     CDS             1063..>1485
                     /locus_tag="PLQ54_19720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS95551.1"
                     /translation="MARLARRLAFAALLVLVWQTVVWLRIWPPLLLPSPGAVVASVVE
                     NVRNGSIPVAIVASMARMLVGYGLALAIGLCTGIATARFRVLQDTVGASAVGLQSLPS
                     ICWLPLALLWFGLTEKAVIFVVVMGATFSIATAVDDGLA"
BASE COUNT          219 a          486 c          498 g          282 t
ORIGIN      
        1 gtcgtcgggg ccttcgttcg agctctggct gacgaggtcc actatgcctc gactgatccc
       61 gaagccctcg ccgccctcaa cgttgagctt gaggcgctca cgaggaagag gttgcccgag
      121 gcgatcctca agccagccat ggagcgggtg cgtttcagca cgattcccga ccccgaagcc
      181 cttcaggcca tggcggacct catgcaccgg gtgggctacg ccaaggaggc catcgacatc
      241 accggactcg tcgacctatc ggtcacccag ccggctgcac cggaggagcg cgccccaaag
      301 tgatctcgct cacctcggtc tcgcgaacct acaccgactc cgctggggcg cgcgccaccg
      361 cagcgatttg cgatgtggac ttggtggtgg acgcgggtca gttcgtgtgt atcgttgggc
      421 cgtcgggatg tgggaagtcg acgctactcg agctcatcgc cggcctccag aagcccgaca
      481 gcggcgaggt gcgcgtgtcg gccacgggcc agtcgaggcc cgtcctgatg ctacaggagg
      541 ccgcactctt cccgtggttg tcggtgaccg gcaacgtcga gttcggcctg gcggtccgtg
      601 gggtacgccg ggcagagcgg cgagaggcag ccctccggca gttggcggcg gttcggctct
      661 cgcgcttcgc ccacgccatg ccacaccaac tctcgggtgg tatgaaacag cgcacggccc
      721 tggcgcgagc tctggccatc gatcccccgg tcctgctcat ggatgaaccc ttcgcgtcgc
      781 tcgatgccca gacgcgcgac ctgatgcacg gcgaactaca gcgcatctgg gccgacacgg
      841 gcaagaccat tgtcttcgtg acccacaacg tccgcgaagc cgtgtgcctg ggcgacaggg
      901 tcttgctgat gtcggcccgg ccgggacgca tcgtcgacgg gattgatgtg gacctgcccc
      961 ggccgcgttc ggtcaccgat cccgcggtct cgcatctggc gcagatcgcg ctcgatcatc
     1021 tgcgtgacga gatcgagaag gtcgagcgcg aggagttcga caatggcacg tctggcccgt
     1081 aggcttgctt tcgccgctct gctggtcctg gtctggcaga ccgtggtctg gctccggatc
     1141 tggccgcccc tgttgctgcc ttcgccgggg gctgtggttg cgtccgtcgt cgagaacgtc
     1201 cgcaatggca gcatccccgt ggccattgtc gcaagcatgg ctcggatgct cgtggggtat
     1261 ggtctggccc tggccatcgg cctgtgcacg ggcatcgcca cggcgcgatt cagggtgttg
     1321 caggacacgg tgggtgcctc agccgtgggc cttcagtcgc ttccgagcat ctgctggctg
     1381 ccgctggcgc tgctatggtt tgggctcacc gagaaagctg tcatattcgt ggtggtgatg
     1441 ggtgcaacat tctccatcgc caccgcggtg gacgacggcc tcgcc
//
LOCUS       DAOSPL010001357         1485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_85920_length_1485_cov_1.434266, whole genome shotgun sequence.
ACCESSION   DAOSPL010001357 DAOSPL010000000
VERSION     DAOSPL010001357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85920_length_1485_cov_1.434266"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLQ54_19725"
     CDS             complement(<1..255)
                     /locus_tag="PLQ54_19725"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOS95552.1"
                     /translation="MANITVMLGLDMETDVGSWGIDYTGVAEGTPKLLDLYGDRGITG
                     TFFFTGEAARRFPDVVRCVANAGHEVGCHSLYHETVGDPIF"
     gene            complement(274..>1485)
                     /locus_tag="PLQ54_19730"
     CDS             complement(274..>1485)
                     /locus_tag="PLQ54_19730"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOS95553.1"
                     /translation="NPAPDDRAVTQGGCRMAGKFAIPKSEFRKRWAALQQKMAEADID
                     VLIAHGDEADPSNVRYLSDYWPLFETGGVAIGRTGDPILLIGPETLTFAKDRGQCDHI
                     MQLLEYRESAEPEYPGVVLDTFQDVIARAAGGKECKRLGIAGLPVMPVTVYESLKKAA
                     GKAKIVRADDLIVELRTIKSANEIQCMREAFRISEVATEYVLNHMRPGMTERQVVGLA
                     QAAMYENGAEYEGHPTYVLSGKASTHAIGRPSDKVLKAGEMIQLNIGALVNGYSSSVG
                     RPVCFGKMPPKMRDLVSFGLEAHHKTAEMMKAGVPAAEVVIRFEEFVKAAGYGSYLLY
                     GPCHAIGLMEVERPWMESSSKYPLAENMTFQVDTFLYTKQFGLRWENGVRVTKTGVEW
                     LSSRFGEVIEL"
BASE COUNT          245 a          492 c          465 g          283 t
ORIGIN      
        1 gaagatgggg tcgccgaccg tctcgtggta gagcgagtgg cagcccacct cgtggccggc
       61 attggcgacg cagcggacca catcggggaa ccgtctggct gcctccccag tgaagaagaa
      121 ggtgcccgta atgcctcgat cgccatacag atccaggagc ttcggtgtgc cctcggcgac
      181 gcccgtgtag tcgatgcccc agctgccgac atcggtctcc atgtcgaggc cgagcatgac
      241 ggtgatgttg gccatgggct tgccccctcg cccctagagc tcgatgacct cgccaaaccg
      301 gctggagagc cactccacgc cggtcttggt aacccggacg ccgttctccc accgcaagcc
      361 gaactgcttg gtgtagagga atgtgtcgac ctggaaagtc atgttctcgg ccagtggata
      421 cttcgagctc gactccatcc acggccgctc cacttccatc agcccgatgg cgtggcaggg
      481 gccgtagagc aggtagctcc cgtaaccggc ggctttcacg aactcctcga aacggatgac
      541 cacctcggcc gcgggaacgc ccgccttcat catctcggcg gtcttatggt gggcctcgag
      601 cccgaagctg accagatcgc gcatcttggg gggcatcttg ccaaagcata cggggcggcc
      661 cacgctcgac gagtagccgt tgaccagggc gccgatgttg agctggatca tctcgccggc
      721 cttcaggacc ttatcgcttg ggcggccgat ggcgtgggtg ctggccttgc cgctcagcac
      781 ataggtcgga tgcccctcgt actcggctcc gttctcgtac attgcggcct gcgcgaggcc
      841 gacgacctgg cgttccgtca tgccaggcct catgtggttc aggacatact cggtcgcaac
      901 ctcgctgatg cgaaaggcct cgcgcatgca ctggatctcg ttggcgctct tgatggtccg
      961 cagctcaacg ataaggtcat cggcgcggac gatcttggcc ttgcccgcag ccttcttcag
     1021 cgactcatac acggtgaccg gcatcacggg gaggccggcg atgcccaggc gcttgcactc
     1081 cttgccgccg gcggccctgg cgatgacatc ctggaacgtg tcgagcacca cgcccgggta
     1141 ctccggctcg gccgactcgc ggtactcgag gagttgcatg atgtggtcac actgcccgcg
     1201 gtccttggcg aaggtgaggg tctccggacc gatcagcagg atcggatcgc cggtgcgccc
     1261 gatggcaacc cccccggtct cgaatagcgg ccagtaatcc gacagatacc gcacgttcga
     1321 tggatcggcc tcgtcaccgt gggcgatgag gacatcgatg tcggcttccg ccatcttctg
     1381 ctggagcgcg gcccagcgct tcctgaactc ggacttcggg atcgcgaact tgcccgccat
     1441 tcggcatcct ccctgggtaa ccgctcgatc gtcgggcgcc gggtt
//
LOCUS       DAOSPL010001358         1483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_86038_length_1483_cov_3.207283, whole genome shotgun sequence.
ACCESSION   DAOSPL010001358 DAOSPL010000000
VERSION     DAOSPL010001358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86038_length_1483_cov_3.207283"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PLQ54_19735"
     CDS             <1..388
                     /locus_tag="PLQ54_19735"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOS95554.1"
                     /translation="QPCEAAQVVAHANANTMLVIQIESQLAIDRVDDIVSTPGLDAVM
                     IGPMDLSLSLGMVGQVDAPRMREAMGSILAACTRHGVACGLHLGDAALLRQWAERGAR
                     FITYSSDIGFVQDGLQEGIHRLKANG"
     gene            407..1189
                     /locus_tag="PLQ54_19740"
     CDS             407..1189
                     /locus_tag="PLQ54_19740"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOS95555.1"
                     /translation="MAKATARTPVDEAIERALPRIIEACQPHLIILYGSRAYGTPRED
                     SDIDLLVITEASGRAVQTAVDAARPAGSPYVSILARAAEDARSRLALGDPVLRDAARA
                     GRVLYDRTGQGLDYLRQPPAHLVDKGDLGLNPMTEEWVRKAETDRVSARALADVVGLQ
                     ANDPVAFHAQQQTEKYLKALLQERGTPPPRTHDLLELLGMVEPPHPGLVVLSSELEAL
                     TPFAVVTRYIGRDTTDDEAAWALNLAERVRAIVRQALGLPLE"
     gene            1204..>1483
                     /locus_tag="PLQ54_19745"
     CDS             1204..>1483
                     /locus_tag="PLQ54_19745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95556.1"
                     /translation="MRATLAMLPTLLLPAAAPAQLDASGGFDAGRRLLSTYFFYRGPW
                     DDQVVTSGGELGMSGTWKLAFPKPDDPAATIYGLDKPERWPGNALPDAS"
BASE COUNT          256 a          509 c          491 g          227 t
ORIGIN      
        1 tcagccctgc gaggccgccc aagtggtcgc tcatgcgaat gccaacacca tgctcgtcat
       61 ccagatcgag agccagctcg ccatcgaccg ggtcgatgac atcgtgtcaa cacccgggct
      121 agatgcggtg atgatcgggc ccatggatct gtcgctgtcg ttgggtatgg tcggacaggt
      181 cgacgccccg cgaatgcgcg aggccatggg cagcatcctg gccgcctgca cccgacacgg
      241 cgtcgcctgc gggctccacc tgggggatgc ggccctgctc cgccagtggg cggaacgcgg
      301 cgcccggttc atcacctact cgagcgacat cggcttcgtg caggacgggc tccaagaggg
      361 aatccaccgg ctcaaggcca atggctaatg cagggaggcg agcagtgtgg ccaaggccac
      421 cgcgcgcaca ccagtcgatg aggccatcga gcgcgcgctg ccgcgcatca tcgaagcctg
      481 ccagccgcat ctgatcatcc tctacggctc ccgggcctat ggcactccgc gggaggacag
      541 cgacatcgat cttctggtga tcaccgaggc ttcggggcgc gcagtccaga ccgcggttga
      601 tgcggcgagg cccgccgggt ccccctatgt gagtatcctg gcgcgagccg ccgaggacgc
      661 acgaagtcgg cttgctctcg gcgacccagt actccgtgac gctgcccgcg ccggccgggt
      721 gctctacgac cgcactgggc aaggcctgga ctacctgagg cagccgcccg cgcacctggt
      781 cgacaaggga gacctcggct tgaacccgat gaccgaggag tgggtacgca aggctgagac
      841 ggatcgcgtg agtgcccggg ccttggccga cgtagtaggt ctgcaggcaa acgaccccgt
      901 ggcattccac gcccagcagc agacagagaa gtacctgaag gcactactgc aggagcgggg
      961 cacgccacct ccccgcactc acgatctgct ggagctgcta ggcatggttg agccccccca
     1021 cccggggctc gtggtgttga gcagcgaact cgaggccctc acgccgttcg cggtggtgac
     1081 acgctacatc gggagggaca caacggacga tgaagcggcc tgggccctca acctcgcgga
     1141 gcgcgtgcga gccattgtcc ggcaggcgct cggactgccc ctggagtgat cggaggcact
     1201 cagttgcgtg caaccctcgc aatgctgccg accctactgc tcccagcggc ggcgcccgcc
     1261 cagctcgacg catcgggcgg gttcgatgcg ggccgccgcc tgctcagcac ctacttcttc
     1321 taccgtggtc cgtgggatga ccaggtcgtc acctcaggcg gggagctggg catgtcgggc
     1381 acgtggaagc tcgccttccc gaagccagat gatcctgccg ccacgatcta cggcctcgac
     1441 aagcccgagc gatggcccgg taacgcgctg cccgacgcct cgg
//
LOCUS       DAOSPL010001359         1483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_86042_length_1483_cov_3.005602, whole genome shotgun sequence.
ACCESSION   DAOSPL010001359 DAOSPL010000000
VERSION     DAOSPL010001359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86042_length_1483_cov_3.005602"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLQ54_19750"
     CDS             <1..487
                     /locus_tag="PLQ54_19750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95557.1"
                     /translation="KAVYYRPGGDPNAPYRLAPGPTPDPAFDTACQFLLSTYAGPQGP
                     DVVFVLPEDTVIGSSDPNRPFGVHTGLTWHAQHIPMVWAGPGIAGGVVSSAPATLADV
                     TPTLCRLLGLRGGPFDGVVLNDALAEGSAADATNQDRATSLLGPVRDALKKASQAEAP
                     E"
     gene            495..>1483
                     /locus_tag="PLQ54_19755"
     CDS             495..>1483
                     /locus_tag="PLQ54_19755"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOS95558.1"
                     /translation="MPQTRRTLLKLFTTGAVVVAAGHRPTVGRDTEGGARVIPVPEPR
                     IARFEELAYGMFVHWGVFSLMGQCVWAMHPGRFPLEDYKRQVPRFTAKEFNPRAWAAT
                     ARRSGMRYMTLTARQHDGFSLYDTRGLSDYDAVHVGPGRDLVAEFVEGCRAEGIEPFL
                     YHTTYDWYQPSFTQDFDAYLEYLHKSIEVLCTYYGKLGGFWFDGNWSKRDADWKEDRL
                     YSIIRRLQPEAVIINNTGVDAPGALGHPEVDCVTFEQSHPTRMDRQGAAKYVAAEMCE
                     SMNSHWGITPNDYNYLSPKGIIEDLCACRGVGANLLLNIGPTPEGRLPAYESAA"
BASE COUNT          267 a          472 c          500 g          244 t
ORIGIN      
        1 gaaggcggtc tactaccggc cgggtggtga cccgaacgcg ccctaccgcc tggcgcccgg
       61 ccctacaccc gatcctgcat tcgataccgc ctgccagttc ctgctgtcaa cctacgcagg
      121 ccctcagggg cccgatgtgg ttttcgtgct gcccgaggac acggtgatcg gttcgtccga
      181 cccgaacagg cctttcggtg tgcacacagg cctgacatgg cacgcgcagc acatccccat
      241 ggtctgggca gggcccggga tcgcgggtgg tgtggtatcg agtgcgcctg ccaccctggc
      301 cgacgtcacc ccgaccctgt gtcgtctact cggcctgcgg ggcgggcctt tcgacggggt
      361 agtcctgaac gatgcgctgg ccgagggaag cgcagcggac gccacgaacc aggacagggc
      421 caccagtctg ctggggccgg tgcgcgacgc gctgaagaag gcgagccagg ccgaggcgcc
      481 ggagtgatct gaggatgccc cagactcgac gcacactgct gaagctattc accaccggtg
      541 cggtcgtggt cgcagccggt catcggccaa cagtcggacg cgacacggaa gggggagcga
      601 gggtgatccc ggtaccggag ccccgtatcg cgcggttcga ggaactcgca tacgggatgt
      661 tcgtgcactg gggagtgttc tcgctcatgg ggcaatgtgt gtgggcgatg cacccgggac
      721 gattcccact cgaggactac aagcgacagg tccctcggtt cacggccaag gagttcaacc
      781 cgcgagcctg ggcggcgacg gcgcgccgga gcgggatgcg gtacatgacg ctgactgcgc
      841 gccagcatga cggcttcagc ctctacgaca cgcggggctt gtcggattac gacgccgtgc
      901 acgtcggccc cgggcgcgat ctcgtcgccg agtttgtgga gggctgccgg gcagagggga
      961 tcgagccgtt cctctaccac accacatacg actggtatca gcccagcttc acccaggact
     1021 tcgacgcgta tctcgagtac ctccacaagt cgatcgaagt gctctgcacc tactacggaa
     1081 agttgggtgg gttctggttc gacgggaact ggagcaagcg ggacgccgac tggaaggagg
     1141 accggctcta cagcatcatc cgccgcctcc agcccgaagc ggtcatcatc aacaacacgg
     1201 gcgtcgatgc tcctggcgcg ctggggcatc cggaggtgga ctgcgtgacg ttcgagcaga
     1261 gccatccgac gcgaatggac cgccagggcg ctgccaagta cgttgcggcg gagatgtgcg
     1321 agtcgatgaa ctcccactgg ggcatcacgc ccaatgacta caactacctt tcgccgaagg
     1381 ggattatcga ggacctctgc gcgtgccggg gagtcggcgc caatctgctc ctgaacatcg
     1441 gcccgacgcc tgagggaagg ctacccgcgt atgagtctgc agc
//
LOCUS       DAOSPL010001360         1482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_86171_length_1482_cov_2.344779, whole genome shotgun sequence.
ACCESSION   DAOSPL010001360 DAOSPL010000000
VERSION     DAOSPL010001360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86171_length_1482_cov_2.344779"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLQ54_19760"
     CDS             complement(<1..247)
                     /locus_tag="PLQ54_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HOS95559.1"
                     /translation="MRDESLEFLKELLWAPSPSGFEEPAQAVVRSYMRPVAQSVRTDV
                     HGNVIAALNETGRPRVMLAGHCDEIGLMVTHVDDQGFL"
     gene            520..1434
                     /locus_tag="PLQ54_19765"
     CDS             520..1434
                     /locus_tag="PLQ54_19765"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOS95560.1"
                     /translation="MSGTQTAFPGRSGESPRWSRVRKSVILAAGRGTRLRPATKAQPK
                     EMLPVGRQPVIGHVRDELALFGVDSILIVTGRDKRAIEDHFDGTDDEDTNGPRIFYVR
                     QSVPKGTADAIAIAEDYAGGEPFIVALGDTIMGVDPREDLLHRMERAFIDTRADVAIA
                     VQAVPPDAVSRYGIVAPKVPLPNDGVGTVEPFVVSDLVEKPPLEEAPSRLAIAARYIF
                     SPVIFDYIRRTPPAANGELQSTDAMRLLLREGGTVCCVPARPGQERHDVGNFENYYEA
                     FITFAMGDEELGPRVRRLVSERLREWPD"
BASE COUNT          247 a          499 c          493 g          243 t
ORIGIN      
        1 cgagaaagcc ctggtcgtcg acatgggtca ccatcaggcc gatctcgtcg cagtggccgg
       61 caagcatgac tcgagggcgt cccgtttcgt tgagcgccgc gatcacattg ccgtgaacgt
      121 cggtgcggac cgactgggcc accggccgca tgtaggagcg caccacggcc tgggccggtt
      181 cctcgaagcc agatgggctg ggggcccaga ggagttcctt caggaactcg agagactcgt
      241 cacgcatgaa tgcctccata tactcgtccc tcccgcgccc gcggagaggg gggctggatc
      301 cgtgtgccca ctccgtaagc ataacgggcc gcccgttgtt cgaacagatc gaccctcggc
      361 tgctcgggtc agcgtcgtcg ccacaccgcg aaccacccgg ggggtgtccc gcgttctgat
      421 cccgtggagg tgtaggggcg gtacagggtc ggtccagccg agcggcactc cctgaagccg
      481 tggccctaac ggaccgagtg gcctggaccg tccccgtgaa tgagcggcac acagaccgcg
      541 ttccccggac gttctggtga gagccccaga tggtcgcgcg tccgcaaatc ggtcatcctc
      601 gcggcgggcc gtggaacccg gcttcgcccc gcgaccaagg cgcagcccaa ggagatgctt
      661 ccggtcggcc gccagcccgt tatcgggcac gtccgcgacg agctggcgct gttcggcgtc
      721 gacagcattc tgatcgtcac cgggcgcgat aagcgcgcca tcgaggacca tttcgatgga
      781 accgacgacg aagacacgaa tggcccgcgg attttctacg tgcggcagag cgtgcccaag
      841 ggaacagctg acgccatcgc cattgccgag gactacgccg gcggcgaacc cttcatcgtc
      901 gccctgggcg ataccatcat gggggtcgac cctcgagagg acctcctcca ccgcatggag
      961 cgggctttca ttgacacgcg ggccgacgta gccatcgcgg tccaggcggt tccgcccgat
     1021 gccgtgtctc gctatggcat cgtcgcgccc aaggtccccc tgcccaacga tggtgtgggc
     1081 accgtcgagc ccttcgtcgt gagcgacttg gtcgagaagc cgcccctcga ggaagcaccg
     1141 agccgcctgg ccatcgcggc gcggtacatc ttttcgcccg tcatcttcga ctacatccgg
     1201 aggacgccgc cggccgcgaa cggagagctg cagagcaccg acgccatgag actactcctc
     1261 cgggaggggg gcaccgtctg ctgtgtcccc gcgcgccccg ggcaagagcg gcacgacgtg
     1321 ggcaacttcg agaactacta tgaggcgttc attaccttcg ccatgggtga cgaggagctg
     1381 ggccccagag tcaggcgtct ggtcagtgag cggctgcggg agtggccgga ctagtgcggt
     1441 cttgtgctgc tctgctccac gtcgcgcagc ggggccccgt cg
//
LOCUS       DAOSPL010001361         1481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_86228_length_1481_cov_3.000701, whole genome shotgun sequence.
ACCESSION   DAOSPL010001361 DAOSPL010000000
VERSION     DAOSPL010001361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86228_length_1481_cov_3.000701"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="PLQ54_19770"
     CDS             complement(<1..977)
                     /locus_tag="PLQ54_19770"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOS95561.1"
                     /translation="MACSRFELRRAVATAALAALLCWTGAGCELSASGNDEPQARAGA
                     DQTVAAGAQVTLDGSASTDADGDALTYAWTQTGGPGVALTGANTAQATFSAPAGSAVL
                     TFLLTVDDGHGGTDSDTVTITVEAATTSRLYVANLIGNSVVGFVDPGLLDGNVAPDAV
                     LSGTATELANPADVVVIGEALAVANFGADSITVYSNAETAHGNEAPRSIVAGANSQLV
                     DPTSLAVDAARDLLYVADVTGADRIMRYAGASLPAFGGDVAPASVITSADLSNPFGVC
                     LAGDELYVANNATNRVAVYARPWTLAGEVAATRVIRCSSFSGIYDVFVDS"
     gene            1226..>1481
                     /locus_tag="PLQ54_19775"
     CDS             1226..>1481
                     /locus_tag="PLQ54_19775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95562.1"
                     /translation="MTAAGIPGGVVGRVLLGGFGPRGLLSYAGLAGRGPRIADAMTSR
                     ERTLTAYRGGQPDRVPVRIWGVEPGMEPPHPVYAPLVAAAQ"
BASE COUNT          220 a          512 c          527 g          222 t
ORIGIN      
        1 gagtccacga acacgtcgta gatccccgag aagctgctgc agcggatcac gcgggtggcg
       61 gcgacctcgc ccgccagcgt ccatggtctg gcgtacaccg cgacgcggtt cgtggcattg
      121 ttggccacgt acagttcatc gccggccagg cataccccga aggggttgct caggtcggca
      181 ctggtgatca cgctggccgg agcaacgtcg ccgccgaagg cgggtagcga ggcgcccgca
      241 taccgcatga tgcggtccgc accggtcacg tcggcgacgt agagcaggtc gcgggccgcg
      301 tccaccgcca ggctcgtggg gtcgaccagc tgcgagttgg ccccggccac aatgcttcgg
      361 ggcgcctcgt tcccatgggc ggtctcagcg ttcgaataca cggtgatgct gtcggcgcca
      421 aagttggcca cggccagagc ctcgccaatc acgaccacat cggcggggtt cgcgagctcc
      481 gtggccgtgc ccgaaaggac ggcgtccggg gccacattgc cgtccagaag ccccggatcg
      541 acgaagccga ccacgctgtt gccgatgagg ttcgccacgt agagacggga cgtggtggcc
      601 gcctcgacgg tgatggtgac cgtgtcagag tcggtcccgc cgtggccgtc atcgacggta
      661 agcaggaacg tgagcacggc ggagccggcc ggcgcggaga acgtggcctg cgcggtgttg
      721 gcgccggtga gcgccacacc cggcccgccg gtctgcgtcc aggcgtaggt cagcgcatca
      781 ccgtccgcgt cggtgctggc cgagccgtcg agagtcactt gggcccccgc cgccacggtc
      841 tggtccgcgc cggcgcgggc ctgtggctca tcgttgccac ttgccgagag ctcgcatccg
      901 gcgcccgtcc agcacagcag agctgcgagg gcggccgttg ccacggcgcg gcggagctcg
      961 aagcgtgaac aggccatagc ggcacctcct acgcggtcgc cggaacacag gcatgcgggc
     1021 cgcgacgggg cagccccacg ggcagaatca ctcacctcgc tgctagtgtt ctacgttggc
     1081 gcgccgagcc attcaagacc tggatatctc cctcggggcg cttgccgggg gccagcgggg
     1141 tccgctccgc gccgccctgc acgattggca accgatctcc gcggccacag gtcccaccga
     1201 cctcgactcc ccccgtggcc gacgcatgac cgcagcgggt atccccggcg gcgtcgttgg
     1261 tcgggtgctg ttgggcggat tcgggccgcg ggggctgctg tcctatgcag gactcgccgg
     1321 acgtggtccg cgaatagctg atgccatgac gagccgagag cggaccctca cagcctatcg
     1381 cggtggccag ccggaccggg tgccggtgcg catatggggt gtcgagcccg gcatggagcc
     1441 gccccatccc gtctacgccc ctctggtggc ggcagctcag g
//
LOCUS       DAOSPL010001362         1480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_86315_length_1480_cov_2.688421, whole genome shotgun sequence.
ACCESSION   DAOSPL010001362 DAOSPL010000000
VERSION     DAOSPL010001362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86315_length_1480_cov_2.688421"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..>1480)
                     /gene="amrB"
                     /locus_tag="PLQ54_19780"
     CDS             complement(183..>1480)
                     /gene="amrB"
                     /locus_tag="PLQ54_19780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOS95563.1"
                     /translation="HAGYEYSASVAAKAYTLLRGAPPGTIVLLGPSHRVPVTGGAIAE
                     YDSWRTPLGEIRVDLDVARGLCRTEPLFRIDSAPHAEEHCLEVQLPFIQRVAPEARIV
                     PVAVGGGAEASGTAQRLADALAAVLRGRDAVVVASSDMSHYPETSDCERVDRAMLVAI
                     ASLDPHRLIVENTRQLESGVRGLECTLCGLSAVEVAVRAVRGLGAHRSQVLAYANSGQ
                     VVPSTRHRSVGYGAVAFLGEGAVAGDGPAALNAAQRARLIAVAREAIRARLRGEPLES
                     GQSTDPVLMHPAAVFVTLTKSGQLRGCIGCTEPELPLMDAVAHYAELAAFSDHRFQPV
                     RDAELPELCIEISVLSPLVRVTSPDDIVLGTHGVMVTQNGRRGLFLPQVATEHSMSRD
                     EFLTRLCTEKAGLPGDAWQHGAQLFVFTVERFSEERGAS"
BASE COUNT          259 a          531 c          486 g          204 t
ORIGIN      
        1 ccgacccgtc gcccgcgccg cagtggggga aaccgtcggg gccgccgcgg acggtccccc
       61 gccttcaggt tgggcgcgca gacagccgcc cagcgagacc gcgccggcta tcgccagctg
      121 ccgcgcggtc cgcctgagga actcgcgccg gcacacaggc ctttgttcca gttccccgat
      181 catcaggaag ctccccgctc ctcgctgaaa cgctccaccg tgaacacaaa gagctgggcc
      241 ccatgctgcc acgcgtcacc aggcaggcct gctttctccg tgcacagacg cgtcaggaac
      301 tcgtcacggc tcatggagtg ctccgtcgcg acctgaggca ggaataggcc ccgccggcca
      361 ttctgggtga ccatcacgcc atgtgtcccc agcacgatat catcgggcga ggttacccgc
      421 accagcggag acagcacact gatctcgatg catagctcgg gcagctcggc atcacgcacc
      481 ggctggaagc gatgatcgct gaatgcggcg agctcagcgt aatgggcgac cgcatccatc
      541 agcggcagct ccggttcggt gcatccgatg cagcctctca actggccgct cttggtcagg
      601 gtgacaaaca cggccgccgg gtgcatcagc actggatccg tggactgccc ggattcgagc
      661 ggttcgccgc gaagacgcgc ccgtatggcc tcccgcgcca ccgcgatcag ccgggcgcgt
      721 tgggcagcgt tcagtgccgc gggtccgtcg cccgcaacgg ccccctcgcc caggaaagcc
      781 acagcgccgt agccaacgga cctgtgtcgc gtcgagggaa ccacttggcc cgagttggcg
      841 taggcgagga cctgcgaccg gtgggcccca aggccacgga cggctcggac ggcaacctcg
      901 acggcgctta gcccgcacaa cgtgcactcg aggccgcgca cgccgctctc gagctgccga
      961 gtgttctcga cgatcagccg atgcgggtcc agcgacgcaa tggccacgag catcgcgcga
     1021 tcgacccgct cgcagtccga tgtttccggg tagtgagaca tgtccgagct cgccacgact
     1081 acggcatcgc ggccgcgcag aacggccgcc agggcatcag cgaggcgctg ggcagtgccg
     1141 gacgcctccg ctccaccgcc aaccgccacg ggcacaatgc gtgcctcagg agccacccgc
     1201 tggatgaagg ggagctggac ctcgaggcag tgctcctcag cgtgaggggc gctgtcgatg
     1261 cggaagagcg gctcggtacg gcacaagccg cgcgccacgt ccaggtccac ccgaatctcg
     1321 cccaagggag tccgccacga gtcatattcc gcgatggccc cgccggtgac cgggacgcgg
     1381 tgactgggcc ccagcagcac gatggtcccg ggcggcgccc cgcgaaggag agtgtaggcc
     1441 ttcgccgcca cagaggcgga gtactcgtag ccagcatgag
//
LOCUS       DAOSPL010001363         1480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_86318_length_1480_cov_2.621053, whole genome shotgun sequence.
ACCESSION   DAOSPL010001363 DAOSPL010000000
VERSION     DAOSPL010001363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86318_length_1480_cov_2.621053"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1072)
                     /locus_tag="PLQ54_19785"
     CDS             complement(122..1072)
                     /locus_tag="PLQ54_19785"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOS95564.1"
                     /translation="MGHWLIPLLCPALIAGAPTWSSTGGKVKVILDTDIGGDIDDAFC
                     LALAIVCPEVELIGVTTSQGPTEAKARIACRMLHLAGRDDVPVAVGRSSPSDYYPQCK
                     WGEGFTAKIPIGQPAADFIIEHVRQAPGEVVLVVVGPETNIMDALAKDPGIAPMVRGI
                     ATMAGSAYVGYNLKPPPDPEFNIKMDVPAAQAMFTSGIPIRMAGLDITAMMRLEADQR
                     AVLFSQKTPLTDALEELYRLWGNETPILYDPVALAWAFDRRFFGVKRSHIEVDAEGYT
                     RVVGGLRPNAEVGVRPDARAFMRFYVDRIVTGCRNRALRR"
     gene            complement(1134..>1480)
                     /locus_tag="PLQ54_19790"
     CDS             complement(1134..>1480)
                     /locus_tag="PLQ54_19790"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOS95565.1"
                     /translation="AGLPAVRFEAVRFTPADGARHYPGVECGGVRVRVSDRDAFESVR
                     TGLHVVCAIRQLWPDRLELPADRVDRLCGLRAVREAIEAGQPAAEIEPLLATGMASFR
                     CQRSAHLLYADP"
BASE COUNT          230 a          524 c          505 g          221 t
ORIGIN      
        1 ggacttcgag gcgctgatgg aagcccagcc ccggatagcc gccctgctgc tccgccggat
       61 ctcgcggatg ctctccctgc gcttgcgccg cacgaacgcg atgctctagc gccccaccgg
      121 cctaacggcg cagcgcccgg ttgcgacagc ccgtgacgat tcgatcgacg tagaagcgca
      181 tgaacgccct ggcatccggc ctcacgccca cctcggcgtt cgggcggagt ccacccacca
      241 cacgcgtgta gccctcggcg tccacctcga tgtggctgcg cttcacgcca aagaaccggc
      301 ggtcgaaggc ccaggccagg gcgacgggat cgtagaggat gggcgtctcg ttgccccaca
      361 gccggtacag ctcctccagc gcgtcggtca gtggcgtctt ctgcgaaaag aggaccgccc
      421 gctgatccgc ctcgagccgc atcatggccg tgatgtcgag cccggccatg cggatgggga
      481 tccccgaggt gaacatggcc tgggcggccg gcacatccat cttgatgttg aactcggggt
      541 cgggtggcgg cttcaggttg tagcccacgt acgcggagcc cgccatggtc gcgatgcctc
      601 tgaccatcgg cgcgattccc gggtccttcg ccagagcgtc catgatgttg gtctcggggc
      661 ccaccacgac cagcacgacc tcaccggggg cctggcgcac gtgctcgatg atgaagtccg
      721 ccgccggctg gccgatgggg atcttggccg tgaagccttc gccccacttg cactgggggt
      781 agtagtccga cgggctcgag cggccgacgg ccacgggcac gtcatcgcgc cccgccaggt
      841 gcagcatgcg gcaggcgatg cgggccttgg cctcggtggg gccctgcgag gtggtcaccc
      901 cgatgagctc gacctcgggg cagacgatgg ccagggccag gcagaaggcg tcatcgatgt
      961 cgccgccgat gtcggtgtcc aggatcacct tgaccttgcc accggtcgag gaccacgtgg
     1021 gtgcccccgc gatcagcgcg gggcagagca aggggatcaa ccagtgtccc aagggactca
     1081 cctctccggc aatccaccgc ccagacgcct cgtaccggcc gggccgcggc acctcatggg
     1141 tcggcgtaga gcagatgggc tgaccgttgg cagcggaagc tcgccatgcc cgtggccagc
     1201 agtggttcga tctctgcggc gggctgccct gcctcgatcg cctcgcgcac cgcgcggagg
     1261 ccacacaggc ggtcgactcg gtccgcgggg agctccaatc ggtccggcca cagctgtcga
     1321 atggcgcaca cgacgtggag gcccgtgcgg acggactcga acgcgtcccg atcgctgacg
     1381 cggacccgaa caccgccgca ctccactccc gggtagtggc gagccccgtc cgctggcgtg
     1441 aatcggaccg cctcgaagcg caccgcgggg agcccggccg
//
LOCUS       DAOSPL010001364         1480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_86327_length_1480_cov_2.404211, whole genome shotgun sequence.
ACCESSION   DAOSPL010001364 DAOSPL010000000
VERSION     DAOSPL010001364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86327_length_1480_cov_2.404211"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1480)
                     /locus_tag="PLQ54_19795"
     CDS             complement(<1..>1480)
                     /locus_tag="PLQ54_19795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95566.1"
                     /translation="VLTTLGSAMAGIDMLCCLIPIMAYPFHYASPENGWSEVVLPWFR
                     SELMVTDRIALANFWSGGSSLYQPENLWPWLMPLALWHVLIAALALMFIAVTALFRRR
                     WIESERLTYPIAQVPMELTLRPERLSRDRWFWLAFGTALCIDVLNGLHVLKPVVPQIP
                     VRSSAYPGFNLGAQLVDRPWNAVGFMAVSFYPFVIGLGMLIPAELSYSCWVFYLLFKI
                     QLVVAQQWGRLEANGFPFTKEQSFGSFVGLVVFSLWTSRGYLRRIGRAALRGGGTRED
                     GEPLSMRAAFALLLAATAGLVAFAIGAGMSAWYAGAFLGIYVLLAQSVTRIRAEMGIP
                     THELYFVGPGQILFRMLGSHFIGPANMGASYLFYWFNRAYRSHPMPHIAEGHKLASRA
                     GLTARSLTAPMLAAMLIGSVAACWSILHLYYRDGAAAKWGPFYHAQWIASAPLSELAG
                     VLQAPTRADPRVIGAVLMGIGATIAGMLGNSRFTGWPLHPIG"
BASE COUNT          307 a          460 c          487 g          226 t
ORIGIN      
        1 tacccgatcg gatggagcgg ccagcccgtg aagcgggagt tccctagcat cccggcgatc
       61 gtggccccga ttcccatgag tactgcaccg atcacccggg gatcggcgcg cgtgggtgcc
      121 tggagcactc ccgccagctc actcaggggc gcgctggcga tccactgggc gtggtagaac
      181 ggcccccatt tggcggcggc gccatcgcga tagtagaggt gcagtatgga ccaacacgcg
      241 gcgacacttc cgatgagcat ggcggcgagc atgggcgccg tgagggagcg cgccgtcagt
      301 ccggctcggc ttgccagctt gtgcccttcg gcgatgtgtg gcatcggatg cgaccggtag
      361 gcccggttga accagtagaa caggtacgat gcacccatat tggcgggacc aatgaagtgc
      421 gagcccagca ttctgaacag tatctggccg ggtcccacga agtagagctc atgagtcggg
      481 attcccatct ccgcccggat gcgggtcacc gactgggcca ggagcacgta gatgccaagg
      541 aacgctccgg cataccacgc gctcattccc gcaccgatgg cgaaggctac gagccccgct
      601 gtggccgcca ggagtagggc gaacgccgcc cgcatcgaga ggggctcccc gtcctcccgg
      661 gtcccacccc cccgcagcgc cgcgcggccg atgcgacgga ggtagccgcg gctggtccac
      721 agcgagaaaa caaccaggcc aacgaaactg ccaaaagact gctctttcgt gaacgggaag
      781 ccattagctt ccagccggcc ccactgctgg gcgaccacca attggatctt gaagagtagg
      841 tagaataccc aacatgagta ggacaactcg gccggtatca gcattcccag cccgataacg
      901 aacgggtaga acgacacggc catgaacccc acagcgttcc acgggcgatc gacaagctga
      961 gcccccaggt tgaagccggg gtaggcagac gagcggacgg ggatctgagg cacgacaggc
     1021 ttgagcacgt ggagtccgtt gagcacgtcg atgcagagag cggtcccgaa ggccagccag
     1081 aaccagcggt ctcggctcag gcgctctggt cgaagtgtga gctccatggg cacttgagcg
     1141 atcgggtatg tcagccgctc ggactcgatc cagcgtctgc ggaacagagc cgtcacggcg
     1201 atgaacatga gtgccagcgc tgcgatgagt acatgccaga gcgccagtgg catcagccac
     1261 ggccataggt tctctggctg atagagcgag ctgcccccgg accagaagtt ggccagggcg
     1321 atgcgatcgg tgaccatcag ctcgctccgg aaccacggga gcaccacctc actccagccg
     1381 ttctcggggc tcgcgtagtg gaaggggtac gccatgatgg ggatgaggca acacagcatg
     1441 tcgatgccgg ccatggccga tccgagtgtt gtgagcacat
//
LOCUS       DAOSPL010001365         1477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_86560_length_1477_cov_3.459916, whole genome shotgun sequence.
ACCESSION   DAOSPL010001365 DAOSPL010000000
VERSION     DAOSPL010001365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86560_length_1477_cov_3.459916"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="PLQ54_19800"
     CDS             <1..1114
                     /locus_tag="PLQ54_19800"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOS95567.1"
                     /translation="LVGVSRSGLYKARRAATRPDGDAELRAQVEPIVGQWPGYGYRRV
                     TRELARHGVVANHKRVLRVMRAEGWLCRLRCRSVRTTQSDHGLGVCPNRIQDLVVTQL
                     DQLWVADLTYIRLPEGFVYLATVLDAYSRRVLGWKLARYLDARLATAALDQALARRPG
                     ARAIHHSDQGVQYASSEYVARLDGAGLVQSMSRRGCPRDNAQAESFFATLKLEEVRLN
                     DYRNFAEAEARLATFIEDVCNGKRMHSALGYRSPVAFEAAERRSKDERSDAASNLPVR
                     KVLALPPANGGYTPASRGPTQTPVPPSIPPTPCLLTGVQSTLLRHQPAPYLPQSHTNS
                     ELSHAYGLAGVVLAAGFTRRAPRALARMWAPMQPAS"
     gene            complement(1018..>1477)
                     /locus_tag="PLQ54_19805"
     CDS             complement(1018..>1477)
                     /locus_tag="PLQ54_19805"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOS95568.1"
                     /translation="WVKLLEGQRAPGSAAHEEYVALGISTGGEYWQAAAAGLNDAGRE
                     LGVRTRFSGPEDHSPEKQAEELDSAIARKPAGILIAPGNPSILTPYIDRAIEHGIPVI
                     CFDTDAPGSKRIAYIGTSNYEAGCMGAHILAKALGARRVNPAAKTTPAKP"
BASE COUNT          227 a          498 c          500 g          252 t
ORIGIN      
        1 gctggtgggc gtgagtcgat cgggtctata caaggcccgt cgggcggcga cccggcccga
       61 tggcgacgcg gagctgcgcg cgcaggtgga gcccatcgtc gggcagtggc cgggttatgg
      121 ctatcggcgg gtgacgcggg agctggcccg ccacggcgtg gtggccaacc acaagcgcgt
      181 gctgcgagtg atgcgcgccg aaggctggct ctgccgcctg aggtgccgga gcgtgcggac
      241 cacccagtcg gatcacggtc tgggcgtgtg ccccaaccgc atccaagacc tggtggtgac
      301 ccaactggac cagttgtggg tggcggacct gacctacatc cgcctgcccg aagggttcgt
      361 gtatctggcc acggtgctgg atgcctattc ccgacgcgtg ctgggctgga agctggcacg
      421 ctacctggat gcgcggctgg ccacggcggc tctggaccag gcactggccc gacgcccggg
      481 cgcccgggcc atccaccact cagaccaggg cgtgcagtat gcgtcgtccg agtatgtggc
      541 gcggttggac ggcgccggac tggttcagag catgagccgt cgagggtgtc ctcgggacaa
      601 cgcccaggcg gagtcgttct tcgccacgct gaagctcgag gaagtgcggc tcaacgacta
      661 ccgcaacttc gccgaagctg aggcccgact ggccaccttc atcgaggatg tctgcaacgg
      721 caagcggatg cactcggctc tgggctaccg gagccccgtg gcgttcgagg cggcggagcg
      781 cagatccaaa gatgagcgga gcgacgccgc ctcgaacttg cccgtcagga aggtactggc
      841 cctcccgccg gcgaacggcg gatacacccc cgcttcgcgg ggcccaaccc aaacacccgt
      901 tcccccctca atccccccaa ctccctgtct actaaccggg gtgcagtcca ctcttctacg
      961 acaccaacct gccccgtacc tccctcaatc tcacaccaac tctgaactct cacacgccta
     1021 cggcttagcc ggcgtggtct tggccgccgg gttcacgcgt cgagcgccga gggctttggc
     1081 gaggatgtgg gcacccatgc agccggcctc gtagttgctg gtcccgatgt aggcgatgcg
     1141 cttgctcccg ggcgcatcgg tgtcgaaaca gatgacgggg ataccgtgct cgatcgcgcg
     1201 gtcgatgtac ggcgtgagga tgctcggatt gccgggcgcg atgaggatgc cggccggctt
     1261 tcgggcgatg gcgctgtcga gttcctcggc ctgcttctcc ggactgtggt cttcggggcc
     1321 gctgaatcgg gttctgacgc cgagctctct cccggcgtcg ttgagaccgg cggcggccgc
     1381 ctgccagtac tcgccgccgg tcgagatacc gagggccacg tactcctcat gggcagcact
     1441 ccccggggct ctctgaccct cgagcagctt cacccac
//
LOCUS       DAOSPL010001366         1477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_86570_length_1477_cov_2.980309, whole genome shotgun sequence.
ACCESSION   DAOSPL010001366 DAOSPL010000000
VERSION     DAOSPL010001366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86570_length_1477_cov_2.980309"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..827)
                     /locus_tag="PLQ54_19810"
     CDS             complement(108..827)
                     /locus_tag="PLQ54_19810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95569.1"
                     /translation="MRSHLRQPLMVAWALALTALLPSCGHAPDVDASLDTAASATPPP
                     GMALVVIGVRWPSLTRELPFLTTRVRVVVTGPGIATPIEGEVAQPASSLVLYVPPGTA
                     RLFSATALDDSGNSLLTAKAENVKITAGVENRVRLTFVLVKGDAALAIDYWPEPLSSS
                     AAASMDWQDLASGSLALTGDWVSRPLSDVSLTGDWVDLSSGSAGISADWQDTSGGSAG
                     LSADWRDSRRSSVGLSVDWSE"
     gene            complement(841..>1477)
                     /locus_tag="PLQ54_19815"
     CDS             complement(841..>1477)
                     /locus_tag="PLQ54_19815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95570.1"
                     /translation="RDADKVWCRFIGSRHLAYLYGSGRLFIVSSRNLSGLDGIWWLED
                     DLSWFLDRVQAASALDDASKWAARGCLTAVYSQQTGAYVRTRIVPTSFARFDGRLRVP
                     DPAPSAARAVLDSLDQGGALYYFDVAGSSLKWKGAEHTYSRVYAQLAPTAGDHGTYVR
                     NYDHRRARVYIEAFFPSGLTADDDFTLISDNGACGIETLVSKHMSELVLGP"
BASE COUNT          252 a          482 c          511 g          232 t
ORIGIN      
        1 tgggggtgag ctgagcggag cgctgggtcg ggggcgtggc tgcacgcggg gggcccgagc
       61 gctccatagg cgcccgatag ccctcacccg atccgctccg gcaggctcta ctcgctccag
      121 tcgaccgaga ggccgacgct gcttcgacgg ctgtcccgcc agtccgcgct gaggccggcg
      181 ctgccaccgg aggtgtcttg ccagtcagcc gagatgccgg cggagcccga ggagaggtcg
      241 acccagtctc ccgtcagaga gacatcggat agcggtcgac tcacccaatc acccgtcagc
      301 gcgaggctgc cgctggccag gtcctgccag tccatggacg cggcggctga gctcgagagc
      361 ggctcgggcc agtaatcgat ggccagggcc gcgtcgccct tcaccagcac gaaagtgagc
      421 cgcacgcggt tctcgacgcc cgcagtgatc ttcacgttct cggccttggc cgtgagcagc
      481 gagttgcccg agtcgtccag cgctgtagcc gagaacagcc gcgcggtgcc cggtggaacg
      541 tagagcacaa gggaggacgc gggctgagcg acctccccct cgatcggagt ggcgatgcct
      601 gggccggtca ccacgacgcg aacgcgcgtg gtgaggaagg gcagctctcg cgttaggctt
      661 ggccaccgga caccgatcac caccagcgcc atgccgggcg gcggcgttgc cgaggccgcg
      721 gtgtcaagac tcgcgtcaac gtcgggcgcg tgcccgcagc tcggaagcag cgcggtgagg
      781 gccagggccc aagccaccat caggggttgt cggaggtgag agcgcatccg gatgtcccct
      841 ctaagggccc agcaccagtt cgctcatatg cttcgagacc agggtctcga tgccgcaggc
      901 accgttgtcg gagatcagcg tgaagtcgtc gtcggcagtg agtcccgacg ggaagaaggc
      961 ctcgatgtag acccgcgctc ggcgatggtc gtagttccgg acgtacgtgc catggtcgcc
     1021 agcagtgggc gctagctgag cgtatacgcg cgagtaggtg tgctcagcgc ccttccattt
     1081 caggctgctg cccgccacgt cgaagtagta caatgcccct ccctgatcta ggctatcgag
     1141 aacagctcgt gccgcagatg gagctgggtc cggaacacgt aggcggccgt cgaaccgcgc
     1201 gaacgatgtg ggcacgatcc ttgttcgcac gtatgctccg gtctgttgcg agtagactgc
     1261 ggtgagacac ccacgcgccg cccacttgct cgcatcgtcc agtgccgatg cggcctggac
     1321 ccggtcaagg aaccaggaca ggtcatcctc gagccaccag ataccgtcga gcccgctcag
     1381 gttccggctg ctgacgatga atagccggcc ggagccgtag aggtacgcga ggtggcggct
     1441 gccgatgaac cggcaccaca ccttgtcggc gtcgcgt
//
LOCUS       DAOSPL010001367         1476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_86658_length_1476_cov_2.611541, whole genome shotgun sequence.
ACCESSION   DAOSPL010001367 DAOSPL010000000
VERSION     DAOSPL010001367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86658_length_1476_cov_2.611541"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..>1476
                     /locus_tag="PLQ54_19820"
     CDS             47..>1476
                     /locus_tag="PLQ54_19820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95571.1"
                     /translation="MYFLLAQALPERLRGRTLYVRVEYFDHGSGTLELQYDALGDGAE
                     SIYRSAGSVTLSGAQSWRAHTFELPQVHFGGRQNGGADFRLWFGAGGASIAVNRLTVT
                     DAAPASAPSVIRPGRDGVVAAVPFRLTWACEQDPDESEIVVSARPGRPASDARATLRV
                     PGPVCTCDVDSSPALRAEDAAAGGTVFVCVRSRWGPRWSTWSEPVPVFVHTGPPGRPI
                     ILPGSTTGLVAWEGEPHTAYEVEVRQGTETRSSGRINSPWSHHGPARSAGGPDAEARV
                     RLMNDAGTGEWSDWTRLGEPAEPPASRRLVGYSPAAYSAIADQDFDYRPVFTEMATWG
                     VNFTRQWVLEAWAAQWPFVRDADGRFDLMRLDEQYLAHMRDLVALANSLGICVQVCLF
                     DHCGLRHRDVYQAHPFRAGNNTQGVDINETNWFDLTAGDGMVGRVHENLARTVTRYLA
                     GYDVVLEVINEPLSGTADQVLDFHRAA"
BASE COUNT          215 a          467 c          536 g          258 t
ORIGIN      
        1 ccgtcgcaac tgggtcgagt gccccgcacg tccaggcggt cagtatatgt atttcctcct
       61 cgcgcaggcc ctgccggagc gtctgcgggg gaggaccttg tacgtgcgtg tcgagtactt
      121 tgaccatggc agcggaacgc tggagttgca gtatgatgct ctcggcgatg gcgcggagag
      181 tatctaccgg tccgcgggct cggtgacgct gtcgggggcg cagtcctggc gcgcacacac
      241 attcgagctg ccgcaggtgc actttggggg gcggcagaac ggcggggcgg acttccggct
      301 gtggttcggt gccggcgggg cgtccatcgc cgtgaatcgc ctgactgtga cggacgccgc
      361 gcctgcgtct gcgccgagcg tgatccggcc cggccgggat ggcgtggtgg ccgcggtacc
      421 gtttcggctg acctgggcgt gcgagcagga cccggatgag tccgagatcg tggtttccgc
      481 gagaccgggg cgccccgcaa gcgacgcgcg cgcgaccctg cgggtgcccg ggccggtttg
      541 cacgtgtgac gtcgactcct ctcctgcgct ccgcgccgag gatgccgcgg ccggaggcac
      601 ggtctttgtc tgtgtccggt cgaggtgggg cccccgatgg agcacgtggt ctgagcccgt
      661 gcccgtcttc gtgcacaccg gcccgcccgg gaggcccatc atcttgcccg gttcgacgac
      721 gggtctcgtg gcctgggaag gcgaaccgca cacggcgtat gaagtcgagg tccggcaggg
      781 cactgagacc agatcgtcgg gacgcatcaa cagcccttgg agccatcacg gccccgcgcg
      841 tagcgcgggt ggcccagacg ccgaggcgcg ggtccggctc atgaatgatg ccggcactgg
      901 agagtggtct gactggacga ggcttggcga accggcagag ccgccagcga gccggcgtct
      961 ggtgggttac agcccggcgg cctactctgc catcgccgac caggacttcg actatcgccc
     1021 cgtgttcacc gagatggcaa cctggggggt gaacttcact cggcagtggg tgctcgaggc
     1081 gtgggctgcc caatggccct tcgtgcgcga tgccgacggc cggttcgact tgatgcgtct
     1141 cgacgagcag tatctggcgc acatgcgcga cctggttgcg cttgccaact cgcttgggat
     1201 ctgtgtgcag gtctgcctgt tcgaccactg cggcctgcgg cacagggatg tctaccaggc
     1261 tcacccgttc cgggccggaa acaacacgca gggcgtcgac atcaacgaga ccaactggtt
     1321 cgacctgacc gcgggcgacg gcatggtggg gcgagtccat gagaacctgg cgcgcacggt
     1381 gacccggtat cttgcgggct acgatgttgt gcttgaggtc atcaacgagc cgctcagtgg
     1441 cacggccgac caggtgctcg acttccaccg agcggc
//
LOCUS       DAOSPL010001368         1475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_86723_length_1475_cov_3.314789, whole genome shotgun sequence.
ACCESSION   DAOSPL010001368 DAOSPL010000000
VERSION     DAOSPL010001368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86723_length_1475_cov_3.314789"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PLQ54_19825"
     CDS             complement(<1..631)
                     /locus_tag="PLQ54_19825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95572.1"
                     /translation="MRNGLNIAGLAIVVLALGLAAYVAFAPSGTIHAQMPPGAAGGAM
                     PPMGGLSAAAPAPGGAAPPSGTEVKTEEGSDYRVSGGTTGIPPAPIYINREGTRSAME
                     AELSRLGAQAMASVPSIYRERLRQDDYIATARANAQEVYSAQIAEACARQAEALGNGD
                     QATATQYAAIAAWLTQERQRMQSGTPTELTSPYYGTHKWFYRSAPSSPPT"
     gene            complement(725..>1475)
                     /locus_tag="PLQ54_19830"
     CDS             complement(725..>1475)
                     /locus_tag="PLQ54_19830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95573.1"
                     /translation="LVSAQGSSPMGPGMMGSGAPGMMGSMGGMGGMGGGAAAPAAPAP
                     STPATEPGPLSEEELALLEWSVASHERWKPLHAELADAALRHAALTDPTLGQLRPGST
                     RAIRYQIESRELPRFAGATRAIEARMERRLSPSSLRQVASGAAARLSPGDWESTHYRA
                     EVRRIHAKAVQKGLALGENLPDSVKADILRSAEAAVRRTTRLKPDDTRWASRVEEERQ
                     DLTVIYVMGPSAAPAVQRPYYRPPSPEDLSR"
BASE COUNT          191 a          519 c          522 g          243 t
ORIGIN      
        1 cagtcggcgg tgaggatggc gccgaccggt agaaccactt gtgggtcccg tagtagggcg
       61 aggtcagctc tgttggcgtc ccggactgca tccgttggcg ctcttgggtc agccatgcgg
      121 cgatggcagc gtattgggtt gctgtggcct ggtcgccgtt gcccaaggcc tcggcctggc
      181 gggcgcatgc ctcggcgatc tgggccgagt agacctcctg ggcgttggcg cgggccgtgg
      241 cgatgtagtc atcctgccgt aggcgttcgc ggtagatgga cggcacactg gccatggcct
      301 gggcgcccag gcggctcagc tcggcttcca tggcggatcg agtgccctca cggttgatgt
      361 agatcggcgc cggagggatg ccggtggtgc cgcccgatac gcggtagtcc gacccctcct
      421 cggtcttgac ttcggtcccc gaaggtggcg ccgcgcctcc gggcgcgggc gctgcggccg
      481 ataggccacc catcggtggc atggccccgc cggcggcacc gggaggcatt tgcgcgtgga
      541 tcgtccccga gggcgcgaac gccacgtagg ccgccaagcc cagggcaagc accacgatcg
      601 ccagaccggc gatattcagc ccgttgcgca cgatctcagc aactcccttc gctcccgagc
      661 cgcccgaggc cacggggaca gcccggctag cgtagaccac ggatgcggcg tgcggcccgg
      721 gggcctaccg cgagagatcc tccggcgatg gtggccggta gtacggcctc tggaccgctg
      781 gggccgccga cggacccatc acatagatca ccgtcaggtc ctgccgctcc tcttcgacac
      841 gactcgccca gcgcgtgtcg tcgggcttca ggcgggtggt gcggcggact gccgcctcgg
      901 cgctgcggag tatgtctgcc ttcaccgagt ccggcaggtt ctcgccgagg gccagcccct
      961 tctgcaccgc tttggcgtgg attcgccgca cttcggcccg atagtgtgtg ctctcccagt
     1021 cgccgggcga cagccgcgct gccgccccag acgccacctg tcgcaggctc gatggcgaca
     1081 accgccgctc cattcgtgct tcgatggcac gtgtcgcccc cgcgaaccgt ggcagctccc
     1141 tggattcgat ctggtagcga atggccctcg tggacccggg gcgcagctgg cccagcgtgg
     1201 ggtcggtgag tgctgcgtgg cgcagtgccg cgtcggccag ctcggcgtgc agtggcttcc
     1261 atcgctcatg agaggccacg gaccattcca gcagggccag ctcctcttcg gagagcggtc
     1321 ccggctcggt cgccggagtg ctgggcgcgg gggcagccgg cgcggccgcg ccgccaccca
     1381 tcccccccat gccacccatg ctgcccatca tgccgggggc gccgctgccc atcatacccg
     1441 gccccatggg gctggagccc tgggcagaaa ccagg
//
LOCUS       DAOSPL010001369         1475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_86739_length_1475_cov_2.699296, whole genome shotgun sequence.
ACCESSION   DAOSPL010001369 DAOSPL010000000
VERSION     DAOSPL010001369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86739_length_1475_cov_2.699296"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..164
                     /locus_tag="PLQ54_19835"
                     /pseudo
     CDS             1..164
                     /locus_tag="PLQ54_19835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012856662.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; incomplete; partial in the middle
                     of a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
     gene            complement(729..1421)
                     /locus_tag="PLQ54_19840"
                     /pseudo
     CDS             complement(729..1421)
                     /locus_tag="PLQ54_19840"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
BASE COUNT          234 a          495 c          514 g          232 t
ORIGIN      
        1 ctgagggcgt gagctgcttc atggtcgcac ccaagtcccc gggccacatg gtgcgctacg
       61 aatacaccca gggcaggggc gtgcccatgc tcatcgcggt cgaggccgac gccaggggcg
      121 acacccgtgc ggtggcgctc tcgtacgcct gcgccatcgg cgggtccggc gagccgcagg
      181 cggtccagcg gcacggtcca tacggactcg ctgacgtcgc tgagagaccc gtcagtggag
      241 taagcatcca tgtcatagta gtagcggact ccggccccga ggttgcggct atggtcggcc
      301 acccagatgg cgttcggatc ggcgacctgc aaagcccgcc ggaacccaag gtctgggtcg
      361 gtcgagcggt agatgttgta cccgccgatg tcggcctccg ggttggccaa ccagaacagc
      421 tcgatctcga tgttggagtc agccgggggg atgccccggg caccggcctt cgcccgcgcc
      481 gcgcgaaggt cgtcgaatac ccgcatccgc gggctggtgt ccggcaggcc ggaagcccgt
      541 gccatggcct cgcgcaccgc caggtatgcg ctcgcgttca catcggcggc ccgcggatag
      601 gtcagtgccc gcggagcccc gtcgaactgg ggcgcgctgg gcagtggctg cgtcgaaggg
      661 atggcgccca ggcggaacga gtaggaggcg gcctgtcccg gcgtgagggc gacagcgctc
      721 tgccccgcgt tgttgctgtc tgtgccgccg gccacgatgt cgtagacggc ggccggcaga
      781 tcggtcagcg agtaggcccc gttggcgtcg gcccgggtaa ccacgctggc cacgccgact
      841 ccgtcgatca cgaggagagc tgagacccag gcttcctgca gcggctgccc ggcggtgctc
      901 agcactgtgc cggataccct ggcgaactgc ccttcagggg ccatcaacag gtgagccgtg
      961 aggtggcgat caccttggtc tcccacgacg acatcgcgca cagcgccgac gaaggtgcgg
     1021 ccgtcgttga gagtcttttc agctaacaca tcatacgttc cgggcgggac gttctcgaac
     1081 acatatgaac cgaactggcc ggtggttgtc tcgaatgagg tgcctgggat ccagacccgg
     1141 gcgccaaaca gagtggagcc atcctcggcg ctgaaagcga cgccacccag cgcgtccgcc
     1201 ccctggcgtt gcagctccag ctggagcacg gactgctcgc ccgaggtcac atcgaactcc
     1261 tcggtgcgtt cgttatagcc agtgaggcga accgtgacca cgtgacgcgt tggccccaga
     1321 tccgggaatg cggccggggt cgtccgaccc gtgtccgcgc cgtcgagcag gatccgcgcc
     1381 cccgctgggg tcgactcgac ccgcgcggat ccctccggca cactggtggc caccaagtcg
     1441 gcctgcgcca ccgtcgtggg gcgatcggcg taggt
//
LOCUS       DAOSPL010001370         1473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_86892_length_1473_cov_3.321580, whole genome shotgun sequence.
ACCESSION   DAOSPL010001370 DAOSPL010000000
VERSION     DAOSPL010001370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86892_length_1473_cov_3.321580"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PLQ54_19845"
     CDS             complement(<1..816)
                     /locus_tag="PLQ54_19845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95574.1"
                     /translation="MSVDPLLIPSILYVFAALCYAAPDLPVEVDGPFSVHVGPGTLEV
                     GGRPVAVSEAVSVAIDPPTWVEVRDERYDALPVFNPQGPPWRKAIPLRGVEAQECAAT
                     GRLDPASVRVKAAAGNGGAVFELGKDYELDGFWGNVGRLEGGAIGEAQPVFVDYRYSP
                     SRIDSIVVDASGEVSVVRGIPHVSMPRQPELPEGVGRAANVWVAGASTELTGDSLFPI
                     LADEYPEPEPITPSEAERLLPKTMAKLRGGETLHVLAWGDSVTVGTFVPDYERN"
     gene            complement(1132..>1473)
                     /locus_tag="PLQ54_19850"
     CDS             complement(1132..>1473)
                     /locus_tag="PLQ54_19850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95575.1"
                     /translation="GTEAVLLYESSGGPISTWPKDRLESFGPVPELLKKCPNQDHYAE
                     WLNAIRGTDTTKGNFEYSGKLAEIVLLGSVAMQAGVPIEYDGEDMEIKDNPKAQALLT
                     KEYRRGWRFGL"
BASE COUNT          246 a          530 c          476 g          221 t
ORIGIN      
        1 attgcgttcg tagtccggca cgaaggtgcc cacggtcacg ctgtcgcccc aggcgagcac
       61 gtggagcgtc tcgccaccgc ggagcttcgc catggtcttc gggagcaagc gctcagcctc
      121 cgacggcgtg atcggctcgg gttccggata ctcatcggcc aggatgggga acaggctgtc
      181 ccccgtcagc tccgtgctgg caccggctac ccagacgttg gccgcccgtc ccacaccctc
      241 gggcaactcg ggctgccggg gcatgctcac atggggtatg ccgcggacca cggatacctc
      301 gccggacgcg tcgaccacga tggagtcgat ccgcgacggg ctgtagcgat agtcgacgaa
      361 cacgggctgg gcctcgccga tagcgccgcc ctccaggcgg cccacattgc cccagaaccc
      421 gtcgagctcg tagtccttcc cgagctcgaa cacggcgccg ccgttcccgg cggcggcctt
      481 cacccgaacg ctggcaggat ccaaccggcc agtggcggcg cattcctgcg cctcgacacc
      541 gcgcagcggg atggctttcc gccacggggg tccctggggg ttgaagacgg gcagcgcgtc
      601 gtagcgctca tcgcgcacct caacccaggt gggcggatct atggcaaccg acacggcctc
      661 gctgaccgcc acaggccgac cgccaacctc gagggtgccg gggcccacat gaacggagaa
      721 gggcccatcg acctccacgg gcaggtctgg cgctgcatag cacagcgcgg cgaacacata
      781 caggatggat ggaatgagca atggatccac agacactcgc ttcctctcgg gccgggccag
      841 ggggcccaca gcgattcgag ttcgcccgcg caaggacggg gtcctcccga tcggagcccc
      901 taagaccgcc acaccagtca cagcgcgttc gacacgtctc ggccgggctg caacaagtag
      961 cgcccctggc atcacagacc tgacaacgcc ccccgtcgag ccccatggcc ttggcgctgc
     1021 ccagtgcgca catgaccgcc gcgaatgcca cacctccacg aggcgccagg ccatgcagag
     1081 agggccccgc ggcggcgccg ggggccctcc cacagacgtg cgcaagctgg actacagccc
     1141 gaagcgccag ccgcggcggt actccttggt caggagtgcc tgagccttcg ggttgtcctt
     1201 gatctccatg tcctcgccgt cgtactcgat cggcacgccc gcctgcatgg cgacgctgcc
     1261 tagcagcacg atctcggcga gcttgcccga atactcgaag ttgcccttgg tggtgtcggt
     1321 gccccggatc gcgttgagcc actcggcata gtggtcctgg ttcgggcact tcttcagcaa
     1381 ctcggggacc gggccgaagc tctccaggcg gtccttgggc caggtcgaga tggggccgcc
     1441 cgagctctcg taaaggagca cggcctcggt gcc
//
LOCUS       DAOSPL010001371         1473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_86893_length_1473_cov_3.319464, whole genome shotgun sequence.
ACCESSION   DAOSPL010001371 DAOSPL010000000
VERSION     DAOSPL010001371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86893_length_1473_cov_3.319464"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1473)
                     /locus_tag="PLQ54_19855"
     CDS             complement(<1..>1473)
                     /locus_tag="PLQ54_19855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95576.1"
                     /translation="ADLRFERRTEPRPHGIDYPFAEQTARGVALFSRFPAFTLSDRRI
                     HVSGPWYSDPAANLHFSRGITSIEAMPNFRLEGAPNSVRAIPAPHKWQLMCDPLFWDY
                     AARLADEMEAADPTDSRIAPLRTFAVDHQFTPHEMAFVALGRRVWLGERGPTDEHLEG
                     VLYPCIDIEQTGGWEHQRDCFGWLYRGMAEAAAENGCRIVPITYGQWTYSVGAVWESM
                     RQGGTGDPEYLLPERDFLAQPDPTLTICEQLGGVISMDGYMQAIWGNEPFYQRQADGS
                     LLLVDGQPVFSEATQTTAYGQPIPLEPGEAEHCLQDLYRQAVRLYLMYHRLAGGYPDR
                     SDQRKAFLRNVRVGAWSRITNEGLQGIQQNDRPLPPWLIETLVMMYLFTADDIVIWSS
                     DTNVPSGPPGADYSQAWKYNAHGVIEYVVKAAHRYSALDPLHQGPFRWCWFRLPMVNK
                     NQTDGDRYFEKPLVFAKLRAFEGQPWIELFAAWPALDGQPA"
BASE COUNT          249 a          458 c          501 g          265 t
ORIGIN      
        1 gcgggctgcc cgtcgagcgc cggccaggcg gcgaacagct cgatccaggg ctggccctcg
       61 aaagcccgga gcttggcgaa caccaggggc ttctcgaagt accgatcccc atcggtctgg
      121 ttcttgttga ccatcggcag ccgaaaccag caccagcgga acggtccctg gtgtagggga
      181 tccagcgcgc tgtaccggtg cgccgctttg accacgtatt cgattacgcc gtgggcgttg
      241 tacttccatg cctggctgta gtcggcaccc ggagggccgc tgggcacatt ggtgtccgat
      301 gaccagatga caatgtcatc ggccgtgaac aggtacatca tcaccagtgt ctcgatgagc
      361 cacggaggga gtgggcggtc gttctgctgg atgccctgta gcccctcatt ggtgatgcgg
      421 ctccatgccc ccacgcggac gttcctgagg aaggctttgc gttggtcact ccggtccggg
      481 tatccaccag cgagtcggtg gtacatcaag tacaatcgga ctgcctgccg gtacagatcc
      541 tggaggcagt gctcggcctc gccgggctcg aggggaatcg gctggccgta agccgtggtc
      601 tgggtggcct cgctgaagac cggctggccg tccacgagca gtaacgaccc gtcggcctgg
      661 cgctggtaga agggctcatt gccccagatc gcctgcatgt agccatccat ggagatgacg
      721 ccgcccagct gctcgcagat ggtcagcgtg gggtccggct gggccaggaa gtctctctcg
      781 ggcaggaggt actcggggtc gccggtcccg ccctgccgca tcgattccca cacggcaccc
      841 acggagtagg tccattggcc ataggtgatg ggcacgatgc ggcatccgtt ctctgccgcg
      901 gcttcggcca tgccgcggta tagccagcca aagcagtcgc gctggtgctc ccacccgccg
      961 gtctgctcga tatcgatgca cgggtagagc accccctcga gatgctcatc cgtaggtccg
     1021 cgctcaccga gccatacgcg gcggcccagc gcgacgaagg ccatctcgtg aggagtgaac
     1081 tggtgatcga cggcaaatgt ccgaagtggg gcgatgcggc tatcggtcgg atcggcggcc
     1141 tccatctcgt cggcgagccg ggccgcatag tcccagaaca gcggatcgca cattagctgc
     1201 cacttgtgcg gggccgggat cgcccgcacg ctgttgggtg caccctcgag ccggaagttc
     1261 ggcattgcct cgatgctggt gatacctcgg gagaagtgaa ggttcgccgc gggatccgag
     1321 taccaggggc ctgacacatg gatgcgccgg tcgctcaggg tgaaggctgg aaagcgcgag
     1381 aagagggcca cgccgcgagc cgtctgctca gcgaaggggt agtcgatgcc gtggggccgt
     1441 ggttccgtgc gcctctcaaa ccgcagatcg gct
//
LOCUS       DAOSPL010001372         1473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_86900_length_1473_cov_2.953456, whole genome shotgun sequence.
ACCESSION   DAOSPL010001372 DAOSPL010000000
VERSION     DAOSPL010001372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86900_length_1473_cov_2.953456"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PLQ54_19860"
     CDS             <1..423
                     /locus_tag="PLQ54_19860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOS95577.1"
                     /translation="VIGYDRYSVDYNDERLARLQREAGKPTLCGEFSMPPWYGGLRGF
                     GRFHSAAEDEAQAGELYNEWTRAAARDPYCVGQIWFMYRDQALTGRGPGRGPQLVHGE
                     HFAFGLITETDRAKWPMVERMREANLAAVRWRMAAPGP"
     gene            529..>1473
                     /locus_tag="PLQ54_19865"
     CDS             529..>1473
                     /locus_tag="PLQ54_19865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95578.1"
                     /translation="MLTLIVYTATLGTAATVEDLPLIDLGITVFQTSSHNKQGLNGDG
                     GYWLYSEEGAEPGLTGWSVVDEGAAPELSLERVHSGRAALCHRVEKTGRESGWRSVVK
                     TLSAGLNVADCDAVTLWVWPTYADGGIDYGIRIDSGGHATELPIRDLRAGEWNEVTVD
                     ISQVPREGVSQFWLLFHVDWGCDAHNAFYVDDIAFRRPDGSTVAIDDFESGQEWRVVL
                     DTLGPGCVRNMWGLGGGRIRIEADGETIVEATQAELFDGKTPLFGPPLVSRKSVSTGP
                     WVAEAHWSFVPIPFLKRCRILTDTPFPFIHYIVERYRDP"
BASE COUNT          234 a          484 c          504 g          251 t
ORIGIN      
        1 gtcatcggct acgaccgcta ctcggtggac tacaacgacg agcggctggc ccggctgcag
       61 cgggaggcgg gaaaaccgac gctgtgcggc gagttcagca tgcccccgtg gtatggcggg
      121 ctgcggggct tcgggcgctt ccactcggcc gccgaggatg aagcccaggc cggggagctc
      181 tacaacgagt ggacccgggc ggcggcgcgg gacccctact gtgtcggcca gatctggttc
      241 atgtaccgcg accaggctct gaccgggcgc gggccgggcc gagggcccca gttggtccac
      301 ggcgagcact tcgccttcgg cctgatcacc gagaccgatc gggccaagtg gcccatggtc
      361 gagcgtatgc gcgaggccaa cctggccgcc gtccgctggc ggatggcggc gcccgggccg
      421 taggcccgta ccccaggacc agtggttcgc tcagatgctt atatggccaa taccgggctg
      481 cttgtatgac catttgctct cgtctggatg gtaaggagcc cccgaatcgt gctgactctc
      541 atcgtctaca cggccacatt gggaaccgcc gccacggtcg aggacctgcc cctaattgac
      601 ctcgggatca ccgtctttca gacctcgagt cacaacaagc aggggctaaa tggtgatggt
      661 ggttactggc tctattccga agaaggtgcc gagcccggac tcaccggctg gtcggtggtc
      721 gacgaggggg cggcgcccga gctgtcactc gagcgagtcc actccggtcg ggccgccctc
      781 tgccaccgtg ttgagaagac gggtcgcgag tccggctggc gatcggtcgt gaaaaccctc
      841 tcggccggcc tcaacgtggc cgactgtgat gcggtcaccc tgtgggtctg gccgacctac
      901 gccgatggcg gcatcgacta cggcatccgg attgacagcg gcggccacgc caccgagctg
      961 cccatccgtg acctgcgggc cggcgagtgg aacgaggtca ctgtggacat cagccaggtg
     1021 ccccgggagg gcgtgagcca gttctggctg ctcttccacg tcgactgggg ctgcgacgcg
     1081 cacaacgcat tctacgtgga tgacatcgcc ttccggcggc ccgatgggtc gacggtcgcc
     1141 atcgacgact tcgagtcggg acaggagtgg cgcgtcgtcc tcgacacgct ggggccgggc
     1201 tgcgtgcgga acatgtgggg tctcggcggc ggacgcatcc ggatcgaggc cgacggcgag
     1261 accatcgtgg aggccacgca ggcggagctc ttcgacggca agacgccgct ctttgggccg
     1321 cccctggtct cgcggaagtc tgtcagcacg ggcccatggg tggccgaggc ccactggtcg
     1381 ttcgtcccca tcccgtttct caagcggtgc cggatcctga cggacacccc cttccccttc
     1441 atccactata tcgtcgaacg ataccgcgat ccg
//
LOCUS       DAOSPL010001373         1473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_86915_length_1473_cov_2.547250, whole genome shotgun sequence.
ACCESSION   DAOSPL010001373 DAOSPL010000000
VERSION     DAOSPL010001373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86915_length_1473_cov_2.547250"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..227
                     /locus_tag="PLQ54_19870"
                     /pseudo
     CDS             1..227
                     /locus_tag="PLQ54_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(535..>1473)
                     /locus_tag="PLQ54_19875"
     CDS             complement(535..>1473)
                     /locus_tag="PLQ54_19875"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOS95579.1"
                     /translation="MLVSLDAHTSMLVADIRGPAVIRHIHTTRHHPPELMARGIVLEI
                     WFDDAKEPAVMSPLADFFGDGCNGASRTFSTPLIECAPWSYNAYIPMPFKKRARVILR
                     NDTDKDAMNYSYVEWEPLDDWDEDLGYFHATYRREQFQLRPDTSHTFFQIEGTGHVIG
                     RQLSILTDEPFFRGFNCVMEGNNEIDVDGRERAFDYLGTEDSFTFSWGFQEEFVGLRS
                     GMPHVVHGDSNSLSIFRFHDHMPIRFDKSLTWRINWQEEKGFVTSPKWQEALARDGSC
                     RVDYATVFYWYQDEPGGFEHAPLAEVALRGNKPAAP"
BASE COUNT          282 a          452 c          500 g          239 t
ORIGIN      
        1 cagcggtcaa aaaagacggc gacaaataca cctacgccag cgaagccgag cccattttgg
       61 ccgaacgcga agccaaagaa gaacaggtgc aaaagctgat ggagatggca cgcaagctcg
      121 aaggcttgac gcgcaatatc ggcatgcacg ccggcggcgt acttatcgcc ccaggcaaac
      181 tcaccgattt ttgcccgctc tatcaacagc cgggcagcag ctcggccgcg ttgacgcgcg
      241 cgagtgtccg ccaggcggca tcggggctcg tgccggcatt ggcgtcggca ccatcggcgg
      301 cgacgtagta gtcgactgcc atggccggga caaggaggag caggaccgcg gcgagggcag
      361 cgggagcgag agaggaggat ggagccatgg gtgggtacct cccaacaggc agtgcagtgt
      421 gccgggcaat cagagccatc acggaccccg gacagtgccg gccaccaggc ccgcgggcac
      481 gagcaccgag accccatgtc ggcgggcgcc ctcggtggcg cggcgactac cgacctacgg
      541 ggccgcgggc ttgttgcccc gcagggccac ttcggccagc ggcgcgtgtt cgaagccacc
      601 cggctcatcc tgataccagt agaacacggt tgcatagtcg accctacacg aaccgtcgcg
      661 ggccagtgcc tcctgccact tcggcgaggt gacgaagccc ttctcctcct gccagttgat
      721 gcgccaggtg aggctcttgt cgaaacggat gggcatgtgg tcgtggaagc ggaagatcga
      781 tagcgagttg ctgtcaccgt ggaccacatg cggcataccc gagcgaaggc cgacgaactc
      841 ctcctggaag ccccagctga aggtgaacga gtcttcagtg cccaggtagt cgaacgcacg
      901 ctcgcggccg tcgacgtcga tctcgttgtt gccctccatc acgcagttga agccgcggaa
      961 gaacggctca tcggtgagga tgctcaactg ccggccgatc acgtggccgg tgccctcgat
     1021 ctggaagaac gtgtggctgg tgtccgggcg gagctggaac tgctcgcggc gataggtcgc
     1081 gtggaagtac cccaagtcct cgtcccagtc gtcgagcggc tcccactcga cgtaggagta
     1141 gttcatcgcg tccttgtccg tatcgttgcg gaggatgacc cgagcccgct tcttgaaggg
     1201 catggggatg taggcgttgt agctccacgg cgcgcactcg atcagtggcg tgctgaaggt
     1261 ccggctggcg ccgttgcagc catcgccgaa gaagtcggcc aggggcgaca tcactgcggg
     1321 ctccttggcg tcgtcgaacc agatctcgag cacgataccg cgcgccatca gctcaggtgg
     1381 atggtggcgc gtggtgtgga tgtggcggat caccgccggg cctctgatgt cggccacgag
     1441 catgctggtg tgggcgtcga gcgacaccag cat
//
LOCUS       DAOSPL010001374         1472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_86983_length_1472_cov_3.130558, whole genome shotgun sequence.
ACCESSION   DAOSPL010001374 DAOSPL010000000
VERSION     DAOSPL010001374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86983_length_1472_cov_3.130558"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PLQ54_19880"
     CDS             <1..720
                     /locus_tag="PLQ54_19880"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOS95580.1"
                     /translation="AGRAEAGRRVAHLPRLRRNHRAGRRAHTGFTLIELLVVIAIIAI
                     LAAILFPVFAKAREKARQASCLSNVKQISNAMMMYIQDYDEQFVTVDETYRWYLPLYA
                     YVKNADVFQCPSNRNDPANSNSDYTINGLFAHGEALARFGQPANTIMIAERETGMVWD
                     GYHPWPNAGSPNWDDLNSYYAGDGHNWLVEHIAKTRHNDGANYGFADGHTKWMKWEKT
                     IEAPLPGLHNPERIVPAAYGW"
     gene            complement(794..>1472)
                     /locus_tag="PLQ54_19885"
     CDS             complement(794..>1472)
                     /locus_tag="PLQ54_19885"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOS95581.1"
                     /translation="DPRALTYSSHAPLGDLLRYYKDRGCKGLGEVMPNLPVMDPMVQN
                     LFKHAQDVGLPVTWDGSDQLTGDFGLYDDPGLPQLEHTLQRWPELIVLGHGPVFWTEI
                     ARLETPGERGHVFRPEGGQVGRVPKGPIKEEGVVPKLLRLYPNLYGDLSDASPWNALA
                     RDPDYGPKFLTEFQDRMLFGTDLCFPTMPIDIIGLLIDWRDTGKISVEVFNKVARENA
                     IRLLGLE"
BASE COUNT          278 a          494 c          451 g          249 t
ORIGIN      
        1 gctggccggg cagaagccgg ccggcgcgtc gcccaccttc cccgactacg acgcaatcat
       61 cgcgcgggcc ggcgcgccca taccggcttc actctcatcg agctactggt cgtgatcgcc
      121 atcatcgcca tcttggccgc gatcctgttc ccggtgtttg ccaaagcacg cgagaaggca
      181 cgacaggcca gttgcctgag caacgtcaag cagatctcga acgcgatgat gatgtacatc
      241 caggactacg atgagcaatt cgtcaccgtc gacgagacat accgctggta cctgcccctc
      301 tacgcctacg tgaagaacgc cgacgtgttc cagtgtccgt cgaaccggaa cgacccggcc
      361 aactcgaact cggactacac catcaacggt ctcttcgccc acggcgaggc actggcgcgg
      421 tttggccagc cggcgaacac gatcatgatc gctgagaggg agaccggcat ggtgtgggat
      481 ggctaccatc cctggcccaa cgccggctcg ccgaactggg acgatctgaa ctcctactat
      541 gcgggcgatg gccacaactg gcttgtcgag cacatcgcca agacgcggca caacgacgga
      601 gccaactatg gcttcgctga cggccacacg aagtggatga agtgggagaa gaccatcgag
      661 gcgccgctgc ccgggcttca caaccccgag cgcatcgtcc cggccgcata cgggtggtag
      721 accgcgggct tctgaggagt ggaccacatg ggccagccga ggtctccttg gctggcccct
      781 tccaggctcc gccctactcc aacccgagta gcctgatcgc gttctcccgc gcgaccttgt
      841 tgaagacctc gacgctgatc ttcccagtgt cccgccagtc gatcagcagg ccgatgatgt
      901 caatcggcat cgtggggaag cataggtcgg tcccaaagag catccggtcc tgaaactcgg
      961 tcaggaactt ggggccatag tcgggatcgc gtgccaacgc gttccagggc gaggcgtccg
     1021 acaggtcgcc gtagaggttc ggatagagcc gtagcagctt ggggaccacg ccctcctcct
     1081 tgatgggtcc ctttggcact cggccgacct gaccgccctc gggccggaac acatgcccgc
     1141 gctcaccggg ggtctccagc cgcgcgatct ctgtccagaa caccggccca tggccgagca
     1201 cgatgagctc cggccagcgc tgcagcgtgt gctcgagctg cggcaggccc gggtcgtcgt
     1261 acagcccgaa atcgcctgtc agttggtctg aaccgtccca cgtgaccggc aaccccacgt
     1321 cctgcgcgtg cttgaacagg ttctggacca tggggtccat cacgggcagg ttgggcatga
     1381 cctcaccgag gcccttacag ccgcggtcct tgtagtaccg caggagatca cctagcggcg
     1441 cgtgggagga gtacgtcaac gcccgggggt cg
//
LOCUS       DAOSPL010001375         1469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_87272_length_1469_cov_2.526874, whole genome shotgun sequence.
ACCESSION   DAOSPL010001375 DAOSPL010000000
VERSION     DAOSPL010001375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87272_length_1469_cov_2.526874"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLQ54_19890"
     CDS             complement(<1..289)
                     /locus_tag="PLQ54_19890"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS95582.1"
                     /translation="MSDKVRIGFVGCGYMGQKAHLDSFVTIPECEIVALAEGRTKTAE
                     MVAAKYRIPRVYPDHRVMLATEKLDAVVAIMYYGLHHAVVPDLLSAGVHVLT"
     gene            490..1260
                     /locus_tag="PLQ54_19895"
     CDS             490..1260
                     /locus_tag="PLQ54_19895"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOS95583.1"
                     /translation="MAGMTCFDVDHESFSGQRDGDVVILHFKHNRMFLTTDLRQTGAL
                     NDFLRRVARDATIRSTAVIGPPAGGGREEYLRFFNRALQPNAPEWVTLRLYNAFDSFI
                     LGAVNSEKAVVVGARGEQPTDYLGLMLACDRRVCSEDLSFVNAHLELGLAPAAGTAFF
                     VAQALGRDRAMELLLSTEPLVASRMLELGLVDEVVPPDELEAATVAAAHRYAAAPAGS
                     IAAVKRLLSFVVRDLPSYLSQETAVADRISSDARSHFS"
BASE COUNT          233 a          517 c          476 g          243 t
ORIGIN      
        1 cagtgagcac atgcacgccg gccgagagca gatcgggcac gaccgcatgg tgcagcccgt
       61 agtacatgat ggccaccacg gcatcgagct tctcggtggc gagcatgacc cggtggtccg
      121 ggtagacgcg cggaatccgg tacttggctg ccaccatctc agccgtcttc gtgcgacctt
      181 ccgccagcgc cacgatctca cactcgggga tggtcacgaa gctgtccaga tgcgccttct
      241 gccccatgta gccgcagccc acgaacccga tgcgaacctt gtcactcaca gagaaccctc
      301 ctcgataggc ccgccgccct ccggcgcccg ggcaggcgga acgccgtggg tttcccgcac
      361 aggggaactt gccccttcgc gggcgcgaga aggccgccaa accgtggcag gttgggcccc
      421 ggcgccggcg aaggctaccg gtgtcagcga catgatttgc atgtcgtcca cactctggga
      481 gggatcggca tggcagggat gacctgcttc gacgtcgacc atgagtcgtt ctccggacag
      541 cgtgacggcg acgtggtgat cctccacttc aagcacaaca ggatgttcct cacaaccgac
      601 ctgcgccaga caggcgccct gaacgacttc ctgcgccgcg tggcccgcga tgcgacgatc
      661 agatcgacgg ccgttatcgg cccgccggcc ggaggcgggc gcgaggagta cctccggttc
      721 ttcaacagag cgctgcagcc caatgcaccc gagtgggtca cgctccggct ctacaatgca
      781 ttcgatagct tcattctggg agccgtgaac tccgagaagg cggtcgtggt cggcgcgcgc
      841 ggtgagcaac cgaccgacta cttgggcctg atgctcgcct gtgatcgccg ggtgtgctcc
      901 gaagacctct cgtttgtcaa tgcgcacctc gaactcggcc tggcgccagc cgctggcacc
      961 gccttcttcg tcgcccaggc tcttggtagg gatcgcgcca tggagctcct gctctcgacc
     1021 gagcccctgg tggcctcccg gatgctcgaa ctgggcctcg tggacgaggt cgtaccgccc
     1081 gatgagctcg aggccgccac cgtcgcggct gctcaccgct atgccgccgc gcccgccggc
     1141 tccatcgccg ccgtgaagag actgctcagc ttcgtggtgc gcgacctgcc ctcgtacctg
     1201 agccaagaga cggcggtggc cgaccggatc tcgtccgacg cccggtcgca cttctcgtag
     1261 tgccctacgc tactgcggtt gcagagacgc ccgccccggc ggcgccgtct tcttcggtga
     1321 caccatcgac cggggggagt tgtgccatgc gcgccatcgt gcggaggctt gcgcggccgc
     1381 ccaacccgat gttgcggagg ggccgcacag gggtgcgccc gcagggcgcg gggcccctga
     1441 ccggcccagt cggccggagg ccgacacag
//
LOCUS       DAOSPL010001376         1466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_87520_length_1466_cov_3.336641, whole genome shotgun sequence.
ACCESSION   DAOSPL010001376 DAOSPL010000000
VERSION     DAOSPL010001376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87520_length_1466_cov_3.336641"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1244)
                     /locus_tag="PLQ54_19900"
     CDS             complement(78..1244)
                     /locus_tag="PLQ54_19900"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOS95584.1"
                     /translation="MAYLDLAERDRRLARMRDIMAAQDLDVALVYYDEFNIGNGWYLT
                     GWCPQFESGAVLVPRVGEPMLLGGPESEPFAKFDSAVKDTRNFTVFMVPNEEYPNATI
                     TDFPALFDEMTQRFGPVRRVGLVGGGRMPAACYQQIVDGFEGVQLVDMTDEFVALRFV
                     KSPWEMEQIRAAFKLADASYEAMAAAIRPGVSEIEVAAAGEFAARTAGATGFGFSAIV
                     GSGPRSNAVVPTATSKRLQAGETVMLGLAPKVAGYAGCFGDTLPVTGECTPQQAEAMK
                     HLKEAFRLTREQLAPGKVGREIDAPARTYFAKHGFSPYLVCPFAHTIGLHEAEEPFFG
                     PNSDHVLKPGMAVCIDVSYFGHPEYLGARIETGYEITDRGAVPFSPAMDARFSS"
BASE COUNT          243 a          495 c          494 g          234 t
ORIGIN      
        1 ggccaacagc agggctatgg ccttggacat gcatccgccg tcattgccgc agatgagcgg
       61 cccctctctc ccatgcacta tgagctgaac cgcgcatcca tcgccgggct gaagggcacc
      121 gcaccgcggt cggtgatctc gtagcccgtc tcgatgcggg cgcccaggta ctcggggtgg
      181 ccgaagtagc tcacatcgat gcacacggcc atgcccggct tcagcacatg gtcgctgttc
      241 gggccaaaga agggctcttc agcctcgtgc agtccgatgg tgtgggcgaa cgggcagacg
      301 aggtacggcg agaatccgtg cttcgcgaag taggtccggg ctggcgcgtc gatctcgcga
      361 ccaaccttgc cgggggcgag ctgctcccgg gtgagccgga aggcctcttt gagatgcttc
      421 atggcctcgg cctgctgcgg cgtgcactcg ccagtgactg gtagggtatc gccgaagcag
      481 ccggcgtatc cggcgacctt gggcgccagg ccgagcatga cggtctcgcc ggcctggagc
      541 cgcttggatg tggcggtggg cacgaccgcg ttcgaccgcg gacccgagcc gacgatggca
      601 ctgaacccga accccgtggc gccggcggtg cgagcggcga actcgcccgc cgccgcgacc
      661 tcgatttccg acacgccagg gcggatcgcg gccgccatgg cctcgtagga ggcatcggcg
      721 agcttgaagg cggcgcggat ctgctccatc tcccacggcg acttcacgaa gcggagcgcc
      781 acgaactcgt cggtcatgtc cacgagctgg acgccctcga aaccgtccac gatctgctgg
      841 tagcaggctg cgggcatccg gccgccgccg acgaggccca cgcggcgcac ggggccaaag
      901 cgctgcgtca tctcgtcgaa cagcgccgga aagtccgtga tggtggcgtt cgggtactcc
      961 tcgttgggga ccatgaagac ggtaaagttg cgggtgtcct tgacggcgct gtcgaacttg
     1021 gcgaagggct cactctccgg cccgcccagg agcatgggct cgcccacacg gggcaccaga
     1081 accgccccac tctcgaactg ggggcaccat ccggtgagat accacccgtt gccaatgttg
     1141 aactcgtcgt agtagaccaa cgcgacgtcc aagtcctggg cagccatgat gtctctcatc
     1201 cgggccagcc gccggtctct ctctgccaga tccaggtatg ccacgcttgg tgcccccttg
     1261 cgtcgggagt gtgcccggag cccagttcgt ggccgggcgc gagccggcct tccacgctta
     1321 cattgccggc aaccgagccg aagccagaga gcgtgccatc agtgccgacg gtcagcagcg
     1381 acgtgcccag cgccccaccc gaggcgatgc gcaggttgcc ggccgtaacg aaggcagggc
     1441 cggtcaggcc gctactatcg ccggtg
//
LOCUS       DAOSPL010001377         1466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_87551_length_1466_cov_2.302622, whole genome shotgun sequence.
ACCESSION   DAOSPL010001377 DAOSPL010000000
VERSION     DAOSPL010001377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87551_length_1466_cov_2.302622"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="PLQ54_19905"
     CDS             complement(<1..924)
                     /locus_tag="PLQ54_19905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95585.1"
                     /translation="MRYFCFALVLATLPTALADARDAAVEGFALHRHRPPDWNWGARP
                     PVGGVAQTNVAPDGSCTVTFTVGERDLPGLDASRVRFGIATGPMADGPTLWLEQGHGV
                     THSGDIPPLDGMVLGGATLISARLGRASGNLVAELVFATPPALESPWDLMGLIDADGE
                     AATGYHGAEWLLQNVALNAEPPPGLSVPWLEARPGIVLRRQPAQVVAWVVNDGPETAQ
                     AVRARLTTARAACRIEPDNPRPFALRPGETRRCTWTVEAAEAGPLALRLEVTSGGRHL
                     QRTRWITVVDERDPRHEFRSQTGDWLRYPERP"
     gene            complement(962..1252)
                     /locus_tag="PLQ54_19910"
     CDS             complement(962..1252)
                     /locus_tag="PLQ54_19910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95586.1"
                     /translation="MTTRPAEGYTRLQIALHWIIAVLVAGQILFGEAMQKFVDAAEEG
                     VGRLLDPLLTEQIAEEIDQTVGIELHRRARFGPAGRHSSSVRGVRRGPAQKP"
BASE COUNT          223 a          498 c          509 g          236 t
ORIGIN      
        1 tggcctctcg ggataccgca gccagtcgcc agtctgtgac cggaactcgt gccgcgggtc
       61 gcgctcgtcg accacggtga tccagcgcgt gcgctgcagg tggcggccgc cactggtgac
      121 ctccagccgc agtgccagcg gcccggcctc ggctgcctca acggtccagg tgcaccggcg
      181 ggtctcgcca ggacggaggg cgaacgggcg cgggttgtcg ggctcgatcc ggcaggccgc
      241 gcgtgccgtg gtgagccggg cgcgcacagc ttgggcggtc tccggaccgt cgttcaccac
      301 ccacgcaacc acctgggccg gttgccgcct gagcacgatg cccggtcgcg cctccagcca
      361 cgggacgctc agcccgggcg gcggctcagc gttgagggcc acgttctgca gcagccactc
      421 ggcgccgtgg taacccgttg ccgcctcgcc gtcggcgtcg atgagcccca tcaggtccca
      481 cggggactcc agggcgggcg gcgttgcgaa gaccagctcg gcgacgaggt tgccactggc
      541 cctgccgagg cgcgccgaga tcagcgtcgc cccccccaac accattccgt cgagcggtgg
      601 gatgtcgccc gagtgtgtga cgccgtgtcc ctgctcgagc cacagggttg gtccgtcggc
      661 catgggccct gtggcgatgc caaaccgcac gcgcgaggcg tcaagcccgg gcaaatcgcg
      721 ctcgcccacg gtgaaggtga cggtgcacga gccgtcgggc gcaacgttgg tctgtgcgac
      781 gccccccacg gggggtcgag cgccccagtt ccagtcgggc ggtcgatggc ggtgcagagc
      841 gaagccctcg acggcggcgt cgcgggcgtc cgccagggcc gtcggtagtg tcgccaggac
      901 aagggcgaag cagaagtagc gcatggaata ccagccttca ttataggtgc agttatactt
      961 cttacggctt ctgcgccgga cctctccgca caccccggac ggaggaggag tgccggccag
     1021 ctgggccgaa ccgggcgcgc cgatgcagct ctatcccgac agtctggtct atctcttctg
     1081 cgatctgttc ggtgaggagt gggtcgagaa gccgtccgac gccctcttcg gccgcatcca
     1141 cgaatttctg catggcctcg ccgaacagga tctggccagc caccagcacg gcaatgatcc
     1201 agtgcagcgc gatctgcagt cgtgtatagc cctcggcggg tcgtgtggtc ataaatgtct
     1261 ccggcgcacc agcggttacg gcccgatagc tgggccgtgc tccgaataag ttcggcgcct
     1321 tacagcatcc tgaacacagg agtccaccca gatacaatgg gcaacactcg tagcagtcga
     1381 cacgaatccg ggaggcagcg tgcggctttg ccgccctacg cttcgctgat tggcggcgcg
     1441 gtggggggtg tgctggcgct cggcct
//
LOCUS       DAOSPL010001378         1465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_87600_length_1465_cov_3.554610, whole genome shotgun sequence.
ACCESSION   DAOSPL010001378 DAOSPL010000000
VERSION     DAOSPL010001378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87600_length_1465_cov_3.554610"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="PLQ54_19915"
     CDS             <1..1115
                     /locus_tag="PLQ54_19915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95587.1"
                     /translation="PFLPRVRNTSTSWGVRMILSDLFDWQIPLTEANWPLLDSTIRER
                     SGDPAWGRQVMQRAGIQRFVTEYVRRGTGQDDALLQYSLEWGFFTRTQWGEYDTALYE
                     LERCWGRQPEPPTPIGAGRRPPTDRTIHSVEDVLAAVEHYVSALPADSILSVATHIST
                     DLDLEPVTEEQMARALARRGMAGPEERNVYASFVNEGFLRALERHYGNRIVVQFSFGA
                     EPLPFETGSRLSQRAIRQLAEMVSRHPGLHFQCFLASRHANQSLCTLCRELPNLSVAG
                     YWWHNFFPSVIRQVIEERLDMLPLNRQVGFFSDAYCVEWAYAKASIVRRQLAAVLAKQ
                     VAQGQYTFDDALTIAQEVLFMAPQQLLGVVPASGTA"
     gene            1192..>1465
                     /locus_tag="PLQ54_19920"
     CDS             1192..>1465
                     /locus_tag="PLQ54_19920"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HOS95588.1"
                     /translation="MYDAARWNSLVDAARQGDRRAYELIYRELSARLYHYAASLVGDA
                     ATAEDITQAAFVKAWESLPRLRSSGAFIVWIHRIVRNLAVDAGRLTA"
BASE COUNT          239 a          508 c          463 g          255 t
ORIGIN      
        1 tgcccttcct cccccgggtg cgcaacacca gcacctcgtg gggcgtccgc atgatcctct
       61 ccgacctgtt cgactggcag atcccactca ccgaggccaa ctggcccctt ttggattcca
      121 ccatccgaga gcgcagtgga gaccccgcct ggggccggca ggtaatgcag cgggccggga
      181 tccagcggtt cgtcaccgag tatgtccgcc gggggactgg tcaggacgac gctttgcttc
      241 agtactccct cgagtggggc ttcttcactc gaacgcagtg gggcgagtac gacacggcgc
      301 tctacgagct cgagcgctgc tggggccggc aacccgagcc gcccacgccg atcggcgccg
      361 ggaggcgtcc tcccacggac cggaccatcc actcggtgga ggatgtcctc gccgccgtcg
      421 agcactacgt gtcggcgctg ccggcggact caatcctctc cgtcgccacg cacatctcca
      481 cggaccttga ccttgagccg gtcacggaag agcagatggc ccgggccctg gcacgccgag
      541 gcatggccgg gccggaggag aggaatgtct acgcgtcgtt cgtcaacgag gggttcctcc
      601 gagccctcga gcggcactac ggcaaccgga tcgtggtcca gttcagcttc ggcgccgagc
      661 ccctgccctt cgagaccggc agccgactca gccagcgagc catccgccag ctggccgaga
      721 tggtcagccg gcacccggga ctgcactttc agtgcttcct cgccagccgg cacgcgaacc
      781 agtccctctg cacgctctgc cgcgagctgc ccaacctctc cgtggcgggt tactggtggc
      841 acaacttctt cccctcggtc attcgccagg tcatcgagga acggctcgac atgttgcccc
      901 tcaatcgcca ggtggggttc ttctcggatg cgtattgcgt cgaatgggcc tatgccaaag
      961 cgagcatcgt ccggcggcaa ctggccgccg tcctggccaa acaggtcgcg caggggcagt
     1021 acacgttcga tgacgctctg accatcgcgc aggaggtgct attcatggcc ccacagcagc
     1081 ttctcggcgt ggtgccagca tcgggtaccg cctagcgcga tccacaccgt gaaacggtgg
     1141 aatggagcgc caccctcacc gtcttctctt ggggtgtagt gaggccgttg gatgtatgac
     1201 gctgcgcggt ggaactcact cgtcgacgcg gcccgccagg gcgatcgccg cgcctacgaa
     1261 ctcatctacc gcgaactgag tgcgcgactc taccattacg cggccagtct ggtcggcgat
     1321 gcggccacgg ccgaggacat cacccaggcc gccttcgtga aggcctggga gtccctaccc
     1381 aggctgagga gctcgggggc attcatcgtg tggattcacc gaatcgtccg gaatctggcg
     1441 gtggacgcgg ggcgccttac cgcgc
//
LOCUS       DAOSPL010001379         1465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_87616_length_1465_cov_2.790780, whole genome shotgun sequence.
ACCESSION   DAOSPL010001379 DAOSPL010000000
VERSION     DAOSPL010001379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87616_length_1465_cov_2.790780"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="PLQ54_19925"
     CDS             <1..1250
                     /locus_tag="PLQ54_19925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95589.1"
                     /translation="VRDFGAETVRIGDLNGDGAPELLFAQSIFRTREITCLTATTVSG
                     EVLWQHGTPSRDNGVIYSDLPVQIYDWDGDGANEVLYVVQARYVEPDEWGGWARERAR
                     SYEGDAFMVILDAATGEEKGRFAIPAPADDCFLFANLTGRGRRQDLVVKDRYWNIWGV
                     SHEGTVLWHWEGSTGHFPAYGDVDDDGKDEIFLGFCLLDHDGTVRFAHDPKGAHQDAA
                     YVVRLEDGTWRLLFGNHGLHCLSIAGEELWSLPMAEAQHVVIARYRGDGPLQCAAIAR
                     GQPRPDGSRDPATLILCDMKGQELWHRELGPESWASGCIAVDWFGADDPSCVLVYGRG
                     PGEPAVLYGGGGELVDSLPMVYAPGHPDNDPRAHFYALKADVWGDGRDEVILFGARGA
                     CIYSNARPLAIPTLYNSNLYPGM"
BASE COUNT          230 a          493 c          502 g          240 t
ORIGIN      
        1 acgtgcgaga cttcggagcc gagaccgttc gcatcggcga tctcaacggc gacggcgcgc
       61 ccgagctgct ctttgcccag agcatcttca ggacccgcga gatcacctgc ctgacggcca
      121 ccaccgtctc tggcgaggtc ctgtggcagc acggcacgcc ctcacgcgac aacggcgtca
      181 tctactccga cctgccggtg cagatctacg actgggacgg cgacggcgcc aacgaggtgc
      241 tgtacgtcgt ccaggcccgg tacgtcgagc ccgacgagtg gggcgggtgg gctcgcgagc
      301 gcgcgcgcag ctacgagggc gacgccttca tggtcatact cgacgccgcc accggcgagg
      361 agaaggggcg gttcgcgatc cccgccccgg ccgacgactg cttcctcttc gcgaacctga
      421 ccggccgcgg ccggcgacag gacctggtcg tcaaggaccg ttactggaac atctggggcg
      481 tgagccacga aggcaccgtc ctgtggcact gggagggctc gaccgggcac tttccggcct
      541 acggcgatgt cgatgacgac ggcaaggacg agatcttcct cggcttttgc ttgctcgacc
      601 acgatggcac cgtgcggttc gcgcacgatc ccaagggagc tcaccaggac gcggcctatg
      661 tggtccggct cgaagatggc acatggcgcc tgctgtttgg caaccatgga ctccattgcc
      721 ttagcattgc gggcgaggag ttgtggagcc ttccgatggc cgaggcgcaa cacgtcgtca
      781 tcgcccgcta ccgtggcgac gggccgctcc aatgtgcggc gatcgcgaga gggcagcctc
      841 ggcccgatgg ttcacgcgat ccggcgaccc tcatactgtg cgacatgaaa gggcaggagc
      901 tgtggcaccg cgagctgggg cccgagagct gggcatcggg gtgcatcgcc gtcgattggt
      961 tcggcgccga cgatccttcg tgcgttctcg tgtacggccg ggggccgggc gagccggcgg
     1021 tgctctatgg cggcggcggc gagctggtcg actcgctgcc tatggtgtac gccccgggcc
     1081 atcccgacaa tgacccgcgt gcccacttct acgccctcaa ggccgacgtc tggggagatg
     1141 gtcgcgacga ggtgatcctg ttcggcgcgc ggggggcctg catctactcc aacgctcgtc
     1201 ccctggccat cccgacgctg tacaacagca atctgtaccc gggtatgtag tgtggttggc
     1261 gtgggcgggc cccatgggga ccgacgagga ccccactccg gcaccgccga gctgagtcga
     1321 gcacgcgtct cgggccgcta tcgaggagcg ggccccgcac cgcctcgccg catcagctcc
     1381 tgcagctcgg acaggtgcgc ctcgaagacg cctcgagggc tggtgcggtg cttcctacac
     1441 agtgaggcgg ccatccccac gacct
//
LOCUS       DAOSPL010001380         1464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_87718_length_1464_cov_2.628105, whole genome shotgun sequence.
ACCESSION   DAOSPL010001380 DAOSPL010000000
VERSION     DAOSPL010001380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87718_length_1464_cov_2.628105"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PLQ54_19930"
     CDS             complement(<1..637)
                     /locus_tag="PLQ54_19930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95590.1"
                     /translation="MRRRWLYLGAALLLASWLGGCKGGGEDEPVSPAPAVTGGRVGEA
                     PTGMAGGDAPVAQGELADGAEPTAVGDASGVSSTPGGTEGGPETPPDGAPGPGEGSQP
                     SGGPEPAKAPPKGDFAMTVRGVTYQLDKAPLAEDKLRQWKVPAYKGAQGATFTPGGPD
                     MGPLAQSAVGMVIKEPPSKVQQFYQGKTKEWKDFRVWVREALAAMQPRGEGA"
     gene            complement(703..>1464)
                     /locus_tag="PLQ54_19935"
     CDS             complement(703..>1464)
                     /locus_tag="PLQ54_19935"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOS95591.1"
                     /translation="HFLLSQQNGVFVGIVSTTRPGWTTILAGPTEAVLADPNAQDDGA
                     GAGGAPDAGGAGMDPEVPVGDPTYGKVPYSVTDPFVRIETTSGTMYVELYQDLAPVTV
                     ANFLKLVDARYYENLTFHRVVRGFMIQGGDDGQGGPDWTIKLEISAIKHARGTLSMAR
                     TNEPDSANAQFFIVHSYRGSRHLDGQYAAFGRVIGGIETVDAIVDAAGGGPGEERIVR
                     GDRAVRILSIERVSWDTVRAALSQKAPDAPAGAGE"
BASE COUNT          207 a          541 c          450 g          266 t
ORIGIN      
        1 cagctccttc gcctctgggt tgcatggccg cgagcgcttc acggacccag accctgaagt
       61 ccttccattc cttggtcttg ccctggtaga actgctggac cttcgagggc ggttccttga
      121 tgaccatgcc cacagcgctc tgggcgagcg gacccatatc ggggccgccg ggcgtgaatg
      181 tggccccctg agctcctttg taagcaggca ccttccactg cctcagtttg tcctcggcga
      241 gtggcgcctt gtcgagctgg tacgtgacgc ccctcacggt catcgcaaag tcacccttgg
      301 gcggtgcctt cgccggctct ggcccacccg aaggctggga gccctctcct ggccctggag
      361 caccatcggg cggcgtctcg ggtccgcctt ccgtgccgcc aggcgtggaa ctcacgccgc
      421 tcgcgtcgcc caccgcggtg ggctcggcgc cgtccgccaa ctcgccctgg gccacagggg
      481 cgtcgccccc cgccataccg gtgggggcct cgcccacgcg cccgcccgtg accgccggcg
      541 ccgggctcac cggctcatcc tcgccgcccc ccttgcagcc cccaagccag ctcgccagca
      601 gcagtgctgc ccccaggtag agccatcgcc gtctcatgtg ttgtgacccc cagccatgat
      661 cggatagaca cgaacgcttg acgtgacgga gcccgccacc cctcattcgc cggcgcccgc
      721 cggtgcgtcc ggcgccttct gggagagggc cgctcgaacc gtgtcccacg agaccctctc
      781 gatcgagagg attctcacgg cccggtcgcc gcgcacgatg cgctcctcgc caggcccacc
      841 gcccgcggcg tccacgatgg cgtcgaccgt ttcgatgcct ccgatcacgc gcccaaaagc
      901 ggcgtactgg ccatctaggt gccgcgatcc ccgatagctg tggacgatga agaactgggc
      961 gttggccgag tctggctcat tcgtccgcgc catcgagagt gtcccacggg cgtgcttgat
     1021 ggccgagatc tcgagcttga tggtccagtc agggccgccc tggccatcgt ctccgccctg
     1081 gatcatgaaa ccgcggacga ctcggtggaa ggtcaggttc tcatagtacc gcgcgtcgac
     1141 tagcttcagg aagttggcca ccgtaacggg ggccagatcc tgatagagct ccacatacat
     1201 cgttccgctc gtcgtctcga ttcgtacgaa cgggtcggtt acgctgtacg gtaccttccc
     1261 gtaggtggga tcacccaccg ggacctcggg gtccattccc gctcctcccg cgtccggagc
     1321 gccgccggcg cccgctccat cgtcctgtgc gttcgggtct gcaagcaccg cttccgtcgg
     1381 acctgccagg atcgtggtcc agcccggccg ggtggtcgag acgatgccca cgaacacgcc
     1441 gttctgctgg ctgagcagga agtg
//
LOCUS       DAOSPL010001381         1464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_87738_length_1464_cov_2.262598, whole genome shotgun sequence.
ACCESSION   DAOSPL010001381 DAOSPL010000000
VERSION     DAOSPL010001381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87738_length_1464_cov_2.262598"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PLQ54_19940"
     CDS             <1..248
                     /locus_tag="PLQ54_19940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95592.1"
                     /translation="LYDISVYWSAAENRTAAQPIIVHDSSGTDHTYYVSLRRNGNEWF
                     PLATLTLGPGSYIEFTTQTPSEGYCNADAVRLMPAGA"
     gene            complement(325..1011)
                     /locus_tag="PLQ54_19945"
     CDS             complement(325..1011)
                     /locus_tag="PLQ54_19945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95593.1"
                     /translation="MRHGAWITVSVVVAVAVGLAAAQDNGYALKDYMPRAVGSIWSMQ
                     PAGGGQRTTLEVLPPEDINGHQVSALVTKSPDGTVRMGTYETVDADSYIVYGSLFVAG
                     PGGPGGGPQGPAPDEPRKVLHDPPARFPGTLKVGETYEATFSSTGGRRPAQVTMKITL
                     EAVESVTVPKGTFDGCLKVVTSTTRGQREMKRTTWYAKGVGPVKTERVGRNGLANVTE
                     LVEYKLGETP"
     gene            complement(1094..>1464)
                     /locus_tag="PLQ54_19950"
     CDS             complement(1094..>1464)
                     /locus_tag="PLQ54_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95594.1"
                     /translation="NGRLLDMFKRMVVDEQDPRTLWLLRGCPRAWLAPGQSVRASDAP
                     TAFGTVGVESRSTEGAVVVQIDPPAQWPPDGLRVVVRRPGGGRWSRITVNGHRAELED
                     GIETVRVRKACRHLTLVCQP"
BASE COUNT          225 a          515 c          462 g          262 t
ORIGIN      
        1 cgttgtatga catctcggtc tactggtcgg ctgcggaaaa ccgaaccgcc gcccagccga
       61 tcatcgtgca cgactcgtcg ggaaccgacc atacctacta tgtcagtctg aggcggaacg
      121 ggaatgagtg gttcccgttg gcgaccctta cactgggccc tggcagttac atcgagttca
      181 ccacacagac tccatcggag ggctactgca acgcggatgc cgtccggctg atgccggcgg
      241 gggcatgaat gggtcgggca gggctgatcc ggcccaggtc gtgatcagcc actgccctcc
      301 cccttcgctc cacgcgccag gctcctatgg ggtctcgccg agcttgtact cgaccaactc
      361 ggtgacgttg gccagcccat tgcggcccac gcgctccgtc ttcactggcc caacgccttt
      421 ggcgtaccaa gtggtgcgtt tcatctcgcg ttgtcctcga gtggtggagg tcacgacctt
      481 gaggcagccg tcgaaggtcc ccttgggcac ggtgactgac tcgacggcct cgagggtgat
      541 cttcatggtc acctgggccg gccgccggcc gccggtggag ctgaaggtgg cctcgtaggt
      601 ttcgccgact ttgagggtgc ccgggaatcg tgcgggcggg tcgtggagga ccttccgggg
      661 ctcatccggg gcggggccct gcgggccccc gccggggccg cccggaccgg cgacgaatag
      721 tgatccatag acgatatagc tgtcggcgtc cacggtctca tacgtcccca tacggaccgt
      781 gccatcgggc gacttcgtca cgagcgccga gacctggtgt ccattgatgt cttcgggcgg
      841 cagtacctcg agtgtcgtgc gctggccgcc accggctggc tgcatcgacc agatcgaccc
      901 tacggctctt ggcatgtagt ccttcagcgc atacccattg tcttgcgctg cggcgagccc
      961 gacggcaact gccacgacga cgctcaccgt gatccatgct ccgtgtcgca tcgtgatgca
     1021 cctccctatg tgctggggcc atggggcccc acaacggccc tgacccacac gctccctcgt
     1081 tccgccgcca gcttcacggc tggcacacca gcgtgaggtg ccgacatgcc ttccgcacgc
     1141 ggacggtctc gatcccatcc tcaagctcgg cccggtggcc attgaccgtt atgcggctcc
     1201 accggccgcc tccgggccgg cgcaccacga cccggagacc atcgggcggc cactgcgccg
     1261 gtgggtcgat ctggactacc acggcaccct ccgtcgatcg ggactcgacg ccaacggtcc
     1321 cgaaggccgt cggggcgtcc gaggcccgaa ccgactgccc cggcgccagc cacgcgcgcg
     1381 gacacccgcg gaggagccac agcgtccgtg gatcctgctc gtcgaccacc attcgcttga
     1441 acatatcgag cagccggccg ttgc
//
LOCUS       DAOSPL010001382         1463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_87795_length_1463_cov_3.580966, whole genome shotgun sequence.
ACCESSION   DAOSPL010001382 DAOSPL010000000
VERSION     DAOSPL010001382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87795_length_1463_cov_3.580966"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PLQ54_19955"
     CDS             <1..528
                     /locus_tag="PLQ54_19955"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOS95595.1"
                     /translation="RGTFVARRRLTHELTKLCSVTADMEARGWRPGAQVLRVQAMAPP
                     AHVRHALGLAQGENVWEVYRLRLADDEPVSLQWCYIPCTLTPGLPVQDLAGSLWRIFS
                     DQYGLKLAFAEQTLRTRRATPDEARLLHIEKGEALFVITRTIYDDQTRPVQYLHSLWR
                     GDRYDFTVRLHCDER"
     gene            complement(698..>1463)
                     /locus_tag="PLQ54_19960"
     CDS             complement(698..>1463)
                     /locus_tag="PLQ54_19960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95596.1"
                     /translation="FSRTAARLRGSMIEVDLPLAGELGADEEAAVYVLAYFHIPTAAK
                     VRLRVDGAWAFRTWIDRSPGPVSSRSAELDLRAGWHELLAKVVVGSEPERLLLAIEPA
                     ERPTTPPVGVGQLMLETRRRPGTDEPPGAERGTRLRGADGPRHSWRMDRPPVGLYDLW
                     IHYAADENSSIAVAVDGYEQEAAASSTGGTGAHRWLKVGVIPIGADLGIRYSPEPAGD
                     PPAAPPQVEVELRGIRGSLWVDRVLLIGDGSSPPPE"
BASE COUNT          208 a          539 c          484 g          232 t
ORIGIN      
        1 cgagggacct tcgtggcgcg acggcggctg acgcacgagc tcaccaagct ctgctcggtg
       61 acggccgaca tggaggcccg gggctggcga ccgggcgccc aggtcctgcg ggttcaggcc
      121 atggcgccgc ccgcgcacgt ccgccacgcc ctcggcctgg ctcagggcga gaacgtgtgg
      181 gaggtctacc ggctgcgcct ggccgatgac gagcccgtca gcctccagtg gtgctacatc
      241 ccctgcacgc tcaccccggg tcttcccgtg caggacctgg cgggatcact gtggcgaatc
      301 ttctcagacc agtacgggct gaagctcgca ttcgccgagc agacccttcg aacccgccgc
      361 gcgacgcctg acgaagcgcg tctcctccac atcgagaagg gcgaggcctt gttcgtcatc
      421 acccggacga tctacgacga ccaaacgcgg ccggtgcagt acctccactc gctctggcgg
      481 ggcgaccggt acgacttcac cgtgcggctg cactgcgacg agcgataggc gatcggtgcc
      541 ccctggggcg gtgactcagg gtccccgagc ggctcctcac ctggttgtgg aggcaccccc
      601 gtgttaccgc tatgcccggt gcgccctgcc agccgcctac ccggtccgga tcgcgaaccg
      661 cggggcatcc catggccgcc acggctcccc ccgtcgctca ctccggcggc ggcgaggaac
      721 cgtcgccgat gagcagcacg cgatcgaccc agagcgaccc tcgtatgccc cgcagttcga
      781 cttccacctg gggtggcgcg gcgggggggt cgcccgcggg ctccggggag taccgaatgc
      841 ccagatcggc gccaatgggt atgacgccca ctttgagcca tcgatgcgcc ccggtcccgc
      901 cggtgctgct tgcggccgcc tcctgctcgt acccgtcgac ggcaacagcg atgctgctgt
      961 tctcgtcggc ggcatagtgg atccataggt cgtacaggcc caccggaggg cggtccatcc
     1021 gccacgagtg acgcggacca tcggcgccgc gaagccgtgt gccgcgttcc gctcccgggg
     1081 gctcgtcggt tcccggcctt cggcgtgtct ccagcatcag ttgccccact cccaccgggg
     1141 gtgtcgtcgg ccgttcggcg ggctcgatgg ccagcagcag gcgctccggt tcggatccga
     1201 ccacgacctt ggccagcagc tcgtgccaac cggcccgaag gtcgagttcg gcggatcggc
     1261 tgctgaccgg cccgggcgac ctatcgatcc acgtgcggaa agcccatgct ccgtccaccc
     1321 gcagccggac cttggccgcg gtcgggatgt ggaagtacgc cagcacgtat acggctgcct
     1381 cctcgtcggc tcccagctct ccggccaagg ggaggtcgac ctcaatcatg cttccgcgta
     1441 gccgcgccgc tgttcgactg aac
//
LOCUS       DAOSPL010001383         1463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_87842_length_1463_cov_2.117188, whole genome shotgun sequence.
ACCESSION   DAOSPL010001383 DAOSPL010000000
VERSION     DAOSPL010001383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87842_length_1463_cov_2.117188"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /gene="argB"
                     /locus_tag="PLQ54_19965"
     CDS             complement(<1..340)
                     /gene="argB"
                     /locus_tag="PLQ54_19965"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOS95597.1"
                     /translation="MDELAVRARTLSEALPYIREFSGGTFVVKYGGSAMASASLSQAV
                     MADVVLLRLVGVRVVLVHGGGPQVSDLMRRLGKEPTFVRGQRVTDAETMEIAEMVLAG
                     KINKDIAAKIQ"
     gene            complement(414..>1463)
                     /gene="argJ"
                     /locus_tag="PLQ54_19970"
     CDS             complement(414..>1463)
                     /gene="argJ"
                     /locus_tag="PLQ54_19970"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOS95598.1"
                     /translation="PCARISRDRVRAGLPVRGVFANSGCANACTGPAGEADIIAIMER
                     TARALGERLPGVDGTVLACQTGVIGRRLTPDRVEDAIDAAASGLSRAGAADAARAIMT
                     TDTRPKECAVEFTVGDRTVRVGGMAKGAGMIAPNMGTMHAFITTDIAVEARALQAALA
                     AAADETFNCITVDGDTSTSDAVLLLANGASGVALDGTSRPAFTEALRVVCQELSQAIV
                     RDGEGATKLVEIRIEGARTSEDARRAARTIAESLLTKTAMFGGDPNWGRILAALGRSG
                     AEVDPNRVVLDLGGLVLFRNGAAESYDLKSAERAVAGPEVAVHLDLGLGAGRATMWTC
                     DLSYDYVRINAEYHT"
BASE COUNT          228 a          536 c          469 g          230 t
ORIGIN      
        1 tctggatctt ggccgcaatg tccttgttga tcttgccggc caggaccatc tccgcaatct
       61 ccatggtctc ggcgtcggtg acgcgctggc cgcgaacgaa ggtgggttcc ttcccgagcc
      121 ggcgcatcag gtccgacacc tggggcccgc ccccgtgaac gagcaccacg cggaccccca
      181 cgaggcgcag cagaaccaca tcggccatga ccgcctggga gagagacgcg ctggccatgg
      241 cgctgccgcc atacttcacc acgaaggtgc cgcccgagaa ctcccggatg tagggcagag
      301 cctcacttag cgtacgggcg cgaaccgcga gttcgtccat ggcgatgatc tcctcgtggg
      361 tctccgtgtt gatggttcgc gcctccctcg gggccgatcc ccccgccgtc atgctaggtg
      421 tgatactcgg cattaatgcg cacatagtca tagctcaggt cgcatgtcca catcgtcgcc
      481 ctgcccgcgc cgaggcccag atcgaggtgg acagcgacct cggggcccgc cacagcccgc
      541 tcggccgact tcaggtcata cgattcggcc gcgccgttgc ggaacagcac caggcccccg
      601 agatcgagca ccacgcggtt cgggtcgacc tccgcccccg agcgccccag agccgcgagg
      661 atgcgacccc agttcggatc gccgccgaac atggccgtct tggtgagcag cgactcggcg
      721 atggtgcgcg ccgcgcggcg agcgtcctcg ctggtgcggg ccccctcgat gcggatctcg
      781 acgagcttgg tcgccccctc gccatcgcgg acgatggcct gactcagctc ctggcagaca
      841 acccgcagcg cctccgtgaa cgcggggcgg ctggtcccat cgagtgccac gcccgaggcg
      901 ccgttcgcca gcaacagcac ggcatcgctc gtgctggtgt cgccgtcgac cgtaatgcag
      961 ttaaacgtct catcagccgc ggcggccagc gcggcctgca gagccctcgc ctccacggcg
     1021 atatccgtcg tgatgaaggc atgcatggtg cccatgttgg gggcgatcat ccccgcaccc
     1081 ttggccatgc cacccactcg aaccgtgcgg tcccccaccg tgaactcgac ggcacactcc
     1141 ttgggccgcg tgtcggtggt catgatggcc cgggccgcgt cggccgcccc cgcccggctg
     1201 aggcctgagg ccgcggcgtc gatggcgtcc tcgactcggt ccggcgtgag gcgtctgccg
     1261 atgacaccgg tctggcaagc cagaacggtg ccatccacac cgggcagacg ctcacccagg
     1321 gcccgggcgg tcctctccat gatggcgatg atgtcggcct cgccggccgg accggtacag
     1381 gcattggcgc aaccgctgtt ggcgaaaacc ccgcgaaccg gcaggccagc gcgcacgcga
     1441 tctctggata tccgcgcgca cgg
//
LOCUS       DAOSPL010001384         1462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_87895_length_1462_cov_3.326937, whole genome shotgun sequence.
ACCESSION   DAOSPL010001384 DAOSPL010000000
VERSION     DAOSPL010001384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87895_length_1462_cov_3.326937"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="PLQ54_19975"
     CDS             complement(<1..1302)
                     /locus_tag="PLQ54_19975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOS95599.1"
                     /translation="MKREILVSTDPRETRLALLEDGQVMEVKVEREASKVGSIYKGIV
                     EAVLPGMDSAFVDVGLERNAFLHASDACAELESESGEGGSNGGRGGGRQRRRRVPPIK
                     DLVQPKQELMLQLTKAPIGDKGARVTTRISLPGRWVVLLPVGANYVGVSKKIEDEEER
                     ARLKEIGHKHRPRGQGIIIRTEAFGCDESEIAEDIEMLKQLWRKLRARYRSRKAPALV
                     HEDLGLTERMIRDYYNDDVTRVLVDTKAEYDRVLEALRNIAPAAAKKAKLYREKESLF
                     EAHGVEAALRDALERKVRLPSGGNITIDETEAIVAIDVNTGRFIGSKGRGLADTILKT
                     NLEAAREIPRQLRLRDLGGIIVLDFIDMYNADHKRQVMETLEERLKRDRARTRVVHLS
                     PLGLVEMTRKRTGQSLLQSSCNVCTRCGGRGRALSPETLAAR"
BASE COUNT          221 a          491 c          467 g          283 t
ORIGIN      
        1 gcgggccgcc agggtctcgg gggaaagcgc ccggccgcgc cccccgcagc gggtgcacac
       61 gttgcacgac gactgcagaa gcgactgccc cgtgcgctta cgggtcatct cgaccagccc
      121 aaggggcgac aggtgcacga ctcgcgttcg agcccgatct cgtttgaggc gctcttcgag
      181 cgtctccatg acctggcgct tgtggtcggc attgtacatg tcgatgaaat cgaggacgat
      241 gatgccgccc agatcgcgca gacgaagctg gcgcgggatc tcgcgagccg cctccaggtt
      301 ggtcttgagg atggtatcgg ccagtccgcg acctttggag ccgatgaagc gcccggtgtt
      361 gacgtcgatg gccacgatgg cctcggtctc gtctatagtg atgttaccac cacttggcag
      421 acgcaccttc cgttcaagtg cgtcgcgcag tgccgcctcg actccgtgtg cttcgaacag
      481 cgactccttc tcgcggtaga gcttggcctt cttggccgcc gcgggcgcaa tgttgcgcag
      541 agcctccagc acgcgatcgt actcggcctt ggtgtcgacg aggacgcggg tgacgtcgtc
      601 gttgtagtag tcgcggatca tgcgctcggt gaggcccagg tcctcatgga ccagcgccgg
      661 agccttgcgg ctgcggtacc gggcccggag tttcctccag agctgcttga gcatctcgat
      721 atcctcggcg atctcgctct cgtcgcaccc gaaggcctcg gtccggatga tgatgccctg
      781 tccgcggggg cggtgcttgt ggccgatctc cttgagtcgc gcgcgctcct cctcgtcctc
      841 gatcttcttc gagaccccga cgtagttggc gcccacgggt agcagcacga cccagcgtcc
      901 gggcagagag atgcgcgtgg tcacgcgagc ccccttgtca ccgatagggg ctttggtgag
      961 ctggagcatc agctcctgtt tgggctggac caggtccttg attgggggga cacggcgccg
     1021 ccgctggcgg ccgccccccc gccccccatt gcttcctccc tcaccgctct cgctctccag
     1081 ctcggcacac gcatccgagg catgcaggaa ggcattacgc tcgagtccga cgtcgacgaa
     1141 ggccgagtcc atgcccggca gcacggcctc tacgatgcct ttgtagatgc taccgacctt
     1201 gctggcctcg cgttcgactt tgacctccat gacctggccg tcctccagca gggcgagacg
     1261 ggtttcacgg gggtctgtgg aaaccagtat ttcacgtttc actgacttga ctcacctcct
     1321 tccgcaacga gtggggcggg ccaactgtgg gtcgggacga ctcagcgccg ccgcagcgac
     1381 cccctggacc gctgcctgtg agcgaacagc gccgtgcggt ggatccacgg caccggttca
     1441 cccggactcc acaggccaag cg
//
LOCUS       DAOSPL010001385         1461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_87987_length_1461_cov_3.358464, whole genome shotgun sequence.
ACCESSION   DAOSPL010001385 DAOSPL010000000
VERSION     DAOSPL010001385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87987_length_1461_cov_3.358464"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="PLQ54_19980"
     CDS             <1..1049
                     /locus_tag="PLQ54_19980"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOS95600.1"
                     /translation="VALETEAEAPVVTHAPLPRLRAAGGRLSGVALARWLRALGAYLL
                     ETGSVDDALRRMAASGAGSSGSMRALQAAVARGKPLAEAMAEQPAHFDALAVELVRAG
                     ERSRRLGPACLEAAEVLEKHRPLTFRAIVFGSNLWSYFTIYLPCTLLIALVALLPVAL
                     VAGLAWLLRLDPVRRWHGLRYSLPILGPLYRSTCFSRFTQAFAACAVVGVPLATSVEL
                     SIRRMGDRQAEATILPRLRSVAHGMKVSEAFRLDPSFPQWVVNVIRTAEETGRMDLAA
                     AKLTDYLQDDTEEAVDRSVGLLLWLLPALVGLVILALLLLAYLGYAGAIGIDMPGPGE
                     AWRNLWSGRRGAGP"
     gene            1071..>1461
                     /locus_tag="PLQ54_19985"
     CDS             1071..>1461
                     /locus_tag="PLQ54_19985"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOS95601.1"
                     /translation="MPQFRYEARTAENQSISGVMQADDERAVVAELVAMGHMVTAVKR
                     ERVAALISPRTGVFDRLFFRARTKDLAFFSRQMAHLLGAGMSVGEALNLLQSRVRNGK
                     LRTALMHMGQRVQDGAPLSGEMRQFPTL"
BASE COUNT          199 a          517 c          510 g          235 t
ORIGIN      
        1 ccgtggcgct cgagaccgag gccgaagctc cggtcgtcac ccacgcgccg ttgccccggc
       61 tgagggcggc gggcgggcgc ctctccggcg tggcccttgc ccgttggctt cgggcgctcg
      121 gggcctacct gctcgagacc ggatcggtcg acgatgccct gcgccggatg gccgcctcgg
      181 gcgccggcag ctccggctcg atgcgcgccc tacaggccgc cgtggcccgg ggcaagccgc
      241 tcgccgaagc catggccgaa cagcccgcgc acttcgacgc gctggccgtc gagctggtac
      301 gggccggcga acgcagtcgg cgactcgggc ccgcatgcct cgaggcggcc gaggtactcg
      361 agaagcatcg ccccctcacc ttccgagcca tcgtgttcgg atccaacctg tggtcatact
      421 tcaccatcta tctaccgtgc acgctgctca tcgcgttggt agcgcttctg ccggtggccc
      481 tcgtggccgg gctggcctgg ctgctgcggc tcgatccggt gcggcggtgg catgggctgc
      541 gctacagcct gcccatactg gggcctctgt accgcagcac ctgcttctcg cggttcaccc
      601 aggccttcgc tgcctgcgcc gtggtcggcg tgccactggc gaccagcgtg gagctctcca
      661 tccggcgcat gggcgaccgc caggccgagg ccacgatcct gccgcggctg cggagcgtgg
      721 cgcatggaat gaaggtctcg gaggccttcc ggctcgatcc cagtttcccg cagtgggtgg
      781 tgaacgtgat ccggacggcc gaggaaaccg gccggatgga cctggcggcg gcgaaactca
      841 cggactacct tcaggatgac accgaagagg ccgtcgaccg cagcgttgga ctgctcctgt
      901 ggcttctgcc cgcactggtg gggttggtca tactggccct gctcctgctg gcctatctgg
      961 gctacgcggg ggccatcggc atcgacatgc ccgggcccgg tgaggcatgg cgcaatctgt
     1021 ggagcggccg ccgcggggcg gggccatagg gccaggagag gggaccacgg atgccgcagt
     1081 tccgctatga ggcgcgcacg gcagagaacc agtcgatcag cggcgtgatg caggccgacg
     1141 acgaacgcgc cgtcgtggcc gaactcgtgg ccatggggca catggtgacc gccgtcaagc
     1201 gagagcgtgt cgcggccctg atctcgccgc gaaccggcgt cttcgaccgt ctcttcttcc
     1261 gcgcgcgcac caaagatctc gccttcttct cccgccagat ggcgcacctc ctcggcgccg
     1321 gcatgtcggt gggcgaggcc ctgaacctgc tccagagccg cgtccgcaac gggaagctgc
     1381 gcacagcgct gatgcacatg gggcagcgcg tccaggacgg cgcccccctg tccggcgaga
     1441 tgcgccagtt ccccacgctg t
//
LOCUS       DAOSPL010001386         1459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_88185_length_1459_cov_3.148860, whole genome shotgun sequence.
ACCESSION   DAOSPL010001386 DAOSPL010000000
VERSION     DAOSPL010001386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88185_length_1459_cov_3.148860"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PLQ54_19990"
     CDS             <1..719
                     /locus_tag="PLQ54_19990"
                     /inference="COORDINATES: protein motif:HMM:NF019952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1727 domain-containing protein"
                     /protein_id="HOS95602.1"
                     /translation="RHFAHIGHFACPKCGHGRVAPDVRVSELKLCGSEALELAVEGET
                     RIEVRVAAGGIYNAYNVAAAVALCQALGITRDRLVEGLEAFRPAFGRVESLEFPDGKR
                     SLLLLAKNPTGMNQVIRLLRDAGGVRHVVIGLNDNIADGRDVSWIWDADFEQLVGVPE
                     SVVVTGIRAWDMAVRLKYAGFAPEQVTVEPDPRAALQVALDRSAPGETVHVVPTYTVL
                     LDVRGKLEREGIAPPFWEEL"
     gene            773..>1459
                     /locus_tag="PLQ54_19995"
     CDS             773..>1459
                     /locus_tag="PLQ54_19995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOS95603.1"
                     /translation="MELRIGHLYADLMSIYGDRGNVLCLAQRARWRGIACTVTPLGLG
                     DALLPEAHDLLFMGGGQDREQRLVAEDFQTAKGQPLREAAEAGVAILAICGGYQLMGH
                     YYRDADGTELPGVGLFDAYTIPGQGPRLIGNVVVQSSVPGLEDRTLVGFENHGGRTHL
                     QGDTRPLGRVVVGGGNNGQDGFEGAVCRNAFGTYLHGSCLPKNPWLADLLIRRALERR
                     YGPVDLAPLDD"
BASE COUNT          227 a          441 c          551 g          240 t
ORIGIN      
        1 cgcgccactt cgcgcacatc ggccacttcg cgtgcccgaa gtgcggccac ggacgcgtgg
       61 cgcccgacgt gcgcgtgtcc gagctgaagc tctgcggcag tgaagccctg gagctcgccg
      121 tcgagggtga gacgcgcatc gaagtccgcg tcgcagcggg tggcatctac aacgcctaca
      181 acgtggcggc agccgtggcg ctgtgccagg ctctgggcat cacgagagac cggctggtcg
      241 agggactcga ggcgttccgg ccggcgttcg ggcgcgtcga aagcctggag tttcccgatg
      301 gcaaacggtc gctgctcctc ctggcgaaga acccgacggg gatgaaccag gtgatacgcc
      361 tgctccgcga tgccggcggc gtgcggcatg tggtgatcgg cctgaatgac aacatcgccg
      421 atgggcgcga tgtctcgtgg atctgggatg cggacttcga gcagcttgtg ggagtgccgg
      481 agtctgtggt ggttaccggc atccgtgcat gggacatggc ggtgcggctc aagtatgcgg
      541 ggttcgcgcc agagcaagtg acggtggagc ccgaccctcg tgcggcgttg caggtggccc
      601 tcgatcgctc agcccccggc gagacggtgc atgtagtgcc gacgtacacg gtgctgctgg
      661 atgtacgtgg gaagctggag cgggagggca tcgcgccgcc gttctgggag gagctgtagg
      721 ggaggtaagc cggtcgttcc cggggagacg cagggcgtgg gaggtgtact gagtggagct
      781 acgcatcggg cacttgtatg ctgacctgat gagcatctat ggcgaccgcg ggaatgtgtt
      841 gtgcctggcg cagcgtgccc ggtggcgggg catcgcgtgc acggtgacgc cgctcggcct
      901 gggcgatgcg ctgctccctg aggcacacga cttgttgttc atgggcggcg ggcaggaccg
      961 cgagcagcgg ctggtggccg aggacttcca gacagccaag gggcagccgc tccgcgaggc
     1021 cgccgaggcc ggagtggcca tcctcgccat ctgcggtggc taccagctga tgggacacta
     1081 ctaccgcgac gccgacggca ccgaactgcc cggcgtgggg ctgttcgatg cctacacgat
     1141 tcccgggcaa gggccacggc tgatcgggaa tgtggtcgtg cagtcctcgg tccccgggct
     1201 cgaggatcgc acgctcgtcg ggttcgagaa ccacggcggc cggacgcact tgcagggcga
     1261 cacccgaccg ctcggcagag tcgtcgtggg cggcgggaac aacggacagg acggtttcga
     1321 gggcgcggtc tgccgcaacg catttgggac ctacctgcac gggtcgtgcc tcccgaagaa
     1381 cccctggctg gccgatctgc tgatccggcg ggccctcgag cgccgctatg ggccagtgga
     1441 cctggcacca ctcgatgac
//
LOCUS       DAOSPL010001387         1459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_88216_length_1459_cov_2.184473, whole genome shotgun sequence.
ACCESSION   DAOSPL010001387 DAOSPL010000000
VERSION     DAOSPL010001387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88216_length_1459_cov_2.184473"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1459
                     /locus_tag="PLQ54_20000"
     CDS             <1..>1459
                     /locus_tag="PLQ54_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95604.1"
                     /translation="VICAQPKAAKIAGSAGAAVSRIRLLAVPDPDALALKRNLPPDDL
                     PRRHLFWREEMADGVVQSRDEAQRGVRDDIAWFEYRAQRMRFLGTDTFCTDLLEFGHN
                     QGWDSAPYGGNDWVNQTPHPQRWEQILDRLRNRGYDFPVLPYYEYAGSIGAKSLGVQR
                     RCRPLTKTGAYTHIDWSEIANADVTDPETLDDVKKVLELTIVRMKDKAHFLGAWFRTR
                     PSHMPMSFGDSALIRFAVEANDHIIATRDDLRADSELLQRYVDWWFGKRRDFLIGIRD
                     YLRDQGIEDAMVLFTPEASEGGPTLPGRQLVTDDPIAWQAAVNEQADLKDLKVTPFDE
                     VVGSGAYLQTVLSPPFTWGEWEWQHSLPQADPQRYTTTDGVALTYPYNRLYTVASPEA
                     FEAFRTAGGLAIIRHYGLNENEMDDQTGYFVCDVERAGPYSMLAEARAVANGDPRYLG
                     FLAGGTFNRGFPQYFRAFVANFLALPALPSRVLEGV"
BASE COUNT          254 a          530 c          446 g          229 t
ORIGIN      
        1 gggtcatttg cgctcagccc aaggccgcca agatcgccgg ctccgcgggg gcagccgtca
       61 gccgcatccg cctcctcgcc gtgcccgacc cggatgccct cgcactgaag cggaacctgc
      121 cgcccgacga cctgccgcgg cggcacctgt tctggcgcga ggaaatggcc gacggcgtgg
      181 tccagagccg cgacgaggcc caacgcggcg taagggacga cattgcctgg ttcgagtacc
      241 gcgcccagcg catgcgcttc cttggtaccg ataccttctg caccgatctg ctcgagttcg
      301 gccacaacca ggggtgggac agcgcgccct acggcggcaa tgactgggtg aaccagaccc
      361 cccacccgca acggtgggag cagatcctcg atcgcctgcg caaccgtggc tacgactttc
      421 ccgtgctccc ctactacgaa tacgcgggta gcatcggcgc caagtccctc ggcgtccagc
      481 gccggtgtcg gccactgacc aagacgggcg cctacacgca catcgactgg tccgagatcg
      541 ccaacgccga tgtcaccgac cccgagacgc tcgacgatgt gaagaaggtc ctcgagctga
      601 ccatcgtgcg gatgaaggac aaggcccact tcctcggcgc ctggttccga acccgcccca
      661 gccacatgcc catgagcttc ggcgatagcg cactcatccg cttcgccgtc gaggccaacg
      721 accacatcat tgccacgcgc gatgacctaa gagccgattc cgagctgctc cagcgctacg
      781 tcgactggtg gttcggcaag cggcgcgact tcctcatcgg catccgcgac tacctgcgcg
      841 atcagggcat cgaagatgct atggtgctgt tcacgccgga agcctccgag ggcggtccca
      901 cactgcccgg cagacagctc gtcaccgatg accccatcgc gtggcaggcc gcggtgaatg
      961 agcaggccga cctgaaggac ctcaaggtca cgccgttcga tgaggtcgtc ggcagcggcg
     1021 cgtacctcca gacggtgctg agtccgccct tcacctgggg cgagtgggag tggcagcact
     1081 cgttgcctca ggccgacccg cagcgctaca cgaccaccga cggcgtggca ctaacctacc
     1141 cgtacaaccg gctctacacc gtggcgtcgc cggaggcctt cgaggccttc cgcacggccg
     1201 gcggcctggc tatcatccgc cactacggcc tcaacgagaa cgagatggac gaccagaccg
     1261 gctactttgt ctgcgacgtc gaacgcgccg ggccctacag catgcttgcc gaggcccgcg
     1321 ccgtcgcgaa tggcgatccg cgctacctcg gtttcctcgc gggcgggacg ttcaaccgcg
     1381 ggttcccaca gtacttccgt gcgttcgtcg ccaacttcct cgcgctgccc gcgctgccga
     1441 gtcgggtgct cgaaggcgt
//
LOCUS       DAOSPL010001388         1459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_88225_length_1459_cov_1.971510, whole genome shotgun sequence.
ACCESSION   DAOSPL010001388 DAOSPL010000000
VERSION     DAOSPL010001388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88225_length_1459_cov_1.971510"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLQ54_20005"
     CDS             complement(<1..223)
                     /locus_tag="PLQ54_20005"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HOS95605.1"
                     /translation="MSSSFDRLTFETTDACPHTHARRGRVRLRRGEFETPAFMPVGTQ
                     ATVKSLTAEDLERLGVRIILASTYHLSQRP"
     gene            complement(228..1235)
                     /locus_tag="PLQ54_20010"
     CDS             complement(228..1235)
                     /locus_tag="PLQ54_20010"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOS95606.1"
                     /translation="MRLEFPFDGPDGSERLDRFVSRVMPEMSRTRIQEAIRNGYILVH
                     QNQERSSYRLRQGDLVVIALPPVDIEPVTVEPEDIPLDILYEDRDILVLNKQAGLVVH
                     PAPGINSGTLVNALLYHFGHLSQSAGLLRPGIVHRLDKDTTGVMVVAKNNEAHDALSS
                     QLAARAFHKVYAALVYGAPRDSGIIDAPIGRHPVDRTKMAIAEHTRRGREAITRYRTV
                     ERFLGMASVSVEIETGRTHQIRVHMTHVGHPVVGDLTYGSGRSRPREISVGEWEGGLK
                     AAVDALPGQALHAESLSFTHPRTGKRLTLVAPWPESMERLRAILRAITAQAPPARQGR
                     R"
     gene            1252..>1459
                     /locus_tag="PLQ54_20015"
     CDS             1252..>1459
                     /locus_tag="PLQ54_20015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95607.1"
                     /translation="MGERRLCFFGFFLTDNAAALSAASATGTGGMGNLSLGAFRNCQI
                     RMPAELAAMAIDAHFLAIKDVFERD"
BASE COUNT          245 a          469 c          481 g          264 t
ORIGIN      
        1 ccggccgctg ggacaggtga taggtgctcg cgaggatgat ccgcacgccg aggcgctcga
       61 ggtcctctgc cgtcagcgac ttcacggtag cctgagttcc caccggcatg aatgccggcg
      121 tctcgaactc gccccgcctc aggcgcacgc ggccccggcg cgcatgcgtg tgcgggcacg
      181 catcggttgt ctcgaaggtc agccggtcga acgagctgct catgggctta gcgacgcccc
      241 tgccgcgccg gaggcgcctg tgccgtgatc gcgcgcagta tggcccggag gcgctccatg
      301 ctctccggcc acggggccac cagggtcaag cgcttgcccg tcctcggatg agtgaatgag
      361 aggctctcgg cgtgcagtgc ctgacccggc agggcgtcaa cagccgcctt gaggccccct
      421 tcccactccc ccactgagat ctctctaggc cggctgcgcc cagacccata ggtgagatcg
      481 cccacaacgg gatggcccac atgggtcatg tggaccctga tctgatgggt ccgtccggtc
      541 tcgatctcaa cggacacact ggccatgccg aggaagcgct cgacggtgcg atagcgcgtg
      601 atcgcctcgc ggccacggcg tgtgtgctcg gcgatcgcca tcttggtccg gtcgactgga
      661 tggcgtccga tcggcgcgtc gataatgccc gagtcccgtg gtgccccgta aactagagcc
      721 gcgtacacct tgtggaacgc gcgggcggcg agctgggacg acagggcatc gtgagcctca
      781 ttgttcttcg cgaccaccat caccccggtg gtgtccttgt caagtcgatg gacgataccg
      841 gggcgaagaa ggcccgccga ctgcgacaga tgcccaaagt ggtacagcag cgcattcacc
      901 agagtgcccg agttgatgcc cggggcggga tgtaccacca gacccgcctg cttattcaac
      961 accaatatat cacgatcttc atacaggatg tcgagcggga tgtcctctgg ctcgaccgtg
     1021 accggctcga tgtctacggg cggcagggcg atgaccacca agtcaccttg gcgaaggcga
     1081 taggacgagc gctcctgatt ctgatggacc agtatgtagc cgtttcggat ggcttcctgg
     1141 atgcgcgttc gcgacatctc cggcatcacg cgggagacga agcggtcgag ccgctcgctg
     1201 ccatcgggcc cgtcgaatgg gaactcgagt cgcataggct atcgcttggg ggtgggagag
     1261 cgccggctgt gcttcttcgg tttcttcttg accgacaacg cggcggccct gtcggcagcc
     1321 tcggcgacgg gcacgggggg aatgggcaac ctctccttgg gggccttccg caactgccag
     1381 atcaggatgc ccgccgagct ggccgcgatg gcgatcgacg cccattttct tgcgatcaag
     1441 gacgtctttg agcgagacg
//
LOCUS       DAOSPL010001389         1457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_88359_length_1457_cov_2.856633, whole genome shotgun sequence.
ACCESSION   DAOSPL010001389 DAOSPL010000000
VERSION     DAOSPL010001389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88359_length_1457_cov_2.856633"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLQ54_20020"
     CDS             <1..409
                     /locus_tag="PLQ54_20020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95608.1"
                     /translation="VGGFRSAHLAGLDPRIKAAVVVGWMCTYGDMLQSHLTSIGPMKI
                     VPGLYQLMDLPDVVSMTCPGALMTISGTKDGLFPLDGVKLAFNKITSVYAKAGVPERF
                     QGVLYEGPHEFNAAMQEKAFDWLDRWIGSASGR"
     gene            447..>1457
                     /locus_tag="PLQ54_20025"
     CDS             447..>1457
                     /locus_tag="PLQ54_20025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOS95609.1"
                     /translation="MLPANPKLSTGLPSLDRVLKGILAGDNVVWQVDHVGDYIPFVEP
                     YCRHATACGTPLVYFRFAKHPPLVAGHPGIDVRVLHPEAGFETFIGEVRQTIRDKGRG
                     AFYVFDCLSDLAVDWYSDQMLGNFFMLTCPYLLDMETVAYFAILRGTHSTQATSAIAD
                     TTQLLLDVLRYQDRLYVHPLKVQHRHSPTMHMLHVWEGDEFHPVRQSAVISEVQTGIA
                     WPQLDSMNYQLGVWNRTFLEAIEVTDPVNADRCSLEHAAQLTQRLLRMVVSRDERILR
                     LAERYLGLPDVLEIGKRMIGTGLIGGKSVGMLLARAILRQSGARWAEVLEPHDSFYIG
                     SDV"
BASE COUNT          249 a          482 c          462 g          264 t
ORIGIN      
        1 cgtgggcggc ttccgctcgg cccatctggc gggcctcgat ccgcgcatca aggcggccgt
       61 ggtcgtcggc tggatgtgca cctacggcga catgctccag tcacacctca ccagcatcgg
      121 gccgatgaag atcgtccccg gactctacca gctgatggac ctgcccgacg tcgtgtcgat
      181 gacctgcccc ggcgccctca tgaccatcag cggtaccaag gacgggctct tcccactgga
      241 tggtgtgaag ttggcgttca acaagatcac gagcgtctat gccaaggcgg gcgtcccgga
      301 gcgattccag ggcgtgctgt acgaggggcc gcacgagttc aacgcagcga tgcaggagaa
      361 ggccttcgac tggctcgacc gatggatagg ctccgcttcc ggccgatagg gcagggggcg
      421 gatgggcctc gggggaaacg gactccatgc tacccgccaa cccgaagctc agcacggggc
      481 tgcccagtct cgatcgggtg ctcaaaggca tactcgccgg cgacaacgtg gtgtggcagg
      541 tggaccacgt cggcgactac atacccttcg tcgagcccta ctgccgccat gccactgcgt
      601 gcggcactcc tctggtctac ttccggttcg ccaagcaccc gccacttgtc gccggccacc
      661 ccggcatcga cgtgcgagtg ctgcatcccg aggcggggtt cgagactttc atcggcgagg
      721 tccgacagac gatccgtgac aaaggccggg gggccttcta cgtctttgac tgtctctccg
      781 acctcgccgt cgactggtat agcgaccaga tgctcggcaa cttcttcatg ctgacgtgtc
      841 cgtatctgct cgacatggag accgtggcct atttcgccat cctgcgcggc acgcactcga
      901 cccaggccac ctcagccatc gccgacacga cccagctcct gctcgacgtg ctgcgttatc
      961 aggacaggct gtacgtgcac ccgctcaagg tgcagcatcg ccactcgccg accatgcata
     1021 tgctgcatgt gtgggagggc gacgagtttc acccggtcag gcagagtgcc gtgatctccg
     1081 aggtgcagac gggcatcgcc tggccgcagc tcgattcgat gaactaccag cttggcgtat
     1141 ggaaccgcac gttcctcgaa gccatcgagg tgacggaccc cgtcaacgcc gaccggtgca
     1201 gcctcgagca tgcggcccag ctcactcagc ggctgttgcg aatggtggtc tcgcgtgatg
     1261 agcgcatcct tcgcctggca gagcgctacc tgggtctgcc ggacgtgctc gagattggga
     1321 aacggatgat cggcacgggc ctcatcggcg gcaagtcggt cggcatgctg cttgcgcgcg
     1381 cgatcctgcg gcagagcggc gcgcgctggg ccgaggtcct ggaaccgcac gactcgttct
     1441 acatcgggtc cgacgtg
//
LOCUS       DAOSPL010001390         1455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_88549_length_1455_cov_2.590714, whole genome shotgun sequence.
ACCESSION   DAOSPL010001390 DAOSPL010000000
VERSION     DAOSPL010001390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88549_length_1455_cov_2.590714"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLQ54_20030"
     CDS             complement(<1..575)
                     /locus_tag="PLQ54_20030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95610.1"
                     /translation="MLITATAGGMTDLSILASPREASIAASAPPGAPDLRQLIAGLGS
                     LDVVETPADELSAQLDEILRGAAAATSARLGSLYVIEQDVLRRVSTTGGNAPACACDR
                     PESDEPWAHCEVLAHVAGTCSVLNLDDVGNLLPGTPDRVARFLRGFQCPRVVSLLAAP
                     IVGPQGEAVGVLELLDSRGVDEQPRPFPPFS"
     gene            complement(621..>1455)
                     /locus_tag="PLQ54_20035"
     CDS             complement(621..>1455)
                     /locus_tag="PLQ54_20035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95611.1"
                     /translation="LIQAQQAASLAADGLRESTANSSLAEWFTLAGMVDRAVHHGLKG
                     VETARQTRAAFAEVYACLKLGAAYLESDEAESAAGYLATAQEVSRALKAPYSSITTAV
                     LQGRLYLARGEALAAEATLLQAIGDADQISARQLAAEAQVWLAAAYVAERRFGEAASA
                     ASAALTRSEDSGDLHTQWQAQYHLAAALEGTSRRDEALYYYRSAVKTIEGMWWPLWSV
                     GFAQVPSVKRSITNAYLGLLDAAETQGNRVEAERVLALSPWPFLRRMWEEHRAARCPT
                     N"
BASE COUNT          236 a          505 c          501 g          213 t
ORIGIN      
        1 tcggagaaag ggggaaaggg ccgaggctgc tcatccacgc cgcgagagtc gagcaattcg
       61 aggacgccca cggcctcacc ctggggccca acgatgggcg ccgccaggag cgacaccacc
      121 cgcgggcact ggaagccccg caggaaccgg gcgacccgat ccggcgtgcc gggcagcagg
      181 ttgcccacgt cgtcgaggtt gagcacgctg cacgtgcccg ccacgtgcgc gagcacctcg
      241 cagtgcgccc atggctcgtc cgattccggc cggtcgcagg cacacgccgg ggcgttgcct
      301 ccggtggtgc tgacccgcct caggacgtcc tgttcgatca cgtacaggga gcccaaccgc
      361 gcgctggtgg cagcggcggc gccgcgcaga atctcatcaa gctgtgccga cagctcgtcg
      421 gcgggtgttt caacgacgtc gagagagccc aggccggcaa tgagctgccg caggtccggt
      481 gccccggggg gcgccgacgc cgcgatactc gcctcgcgcg gagacgctag gatcgacagg
      541 tctgtcatgc cgccagcggt agccgtgatg agcaactccc attcaaccgg ctcaacccgc
      601 gatgcgggct gagcggaggc ctagttcgtg gggcaccggg ccgcccggtg ctcctcccac
      661 atgcgccgca ggaatggcca gggcgagagc gcgagaacgc gctcggcctc gacccgattc
      721 ccctgagttt cggccgcgtc caacagtccc aggtaggcgt tggtgatcga gcgcttgacg
      781 ctcggaacct gagcgaatcc cactgaccac agcggccacc acatgccctc gatggtcttg
      841 acggcactgc ggtagtagta gagggcctcg tcgcgccgtg atgtaccttc gagggccgct
      901 gccaggtggt actgcgcctg ccactgggta tgaaggtcgc cggagtcctc ggagcgcgtc
      961 agcgccgccg aggctgcgga agccgcctca ccgaagcgtc gctcggccac ataagcagcg
     1021 gcaagccaga cctgcgcctc ggcagccagc tgtctggcgc tgatctggtc cgcatcgccg
     1081 atcgcctgga gaagagtcgc ctcggcagcc aaggcctccc cgcgagccag gtagagcctc
     1141 ccctgaagta cggccgtggt gatgcttgag tagggagcct tcagggcccg ggagacctcc
     1201 tgcgccgtgg ccagataccc ggcggccgac tcggcttcgt cggattcgag atacgcggct
     1261 ccgagcttga ggcaggcgta cacctcggcg aatgccgccc gggtctggcg ggccgtttcc
     1321 acgcccttga gcccgtggtg cacggcccga tcgaccatcc cggccagcgt gaaccattcc
     1381 gccagcgagc tgttggccgt ggactcccga aggccgtcgg ccgcgagtga cgcggcctgt
     1441 tgcgcctgaa tgagc
//
LOCUS       DAOSPL010001391         1454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_88610_length_1454_cov_3.351680, whole genome shotgun sequence.
ACCESSION   DAOSPL010001391 DAOSPL010000000
VERSION     DAOSPL010001391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88610_length_1454_cov_3.351680"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PLQ54_20040"
     CDS             <1..533
                     /locus_tag="PLQ54_20040"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOS95612.1"
                     /translation="DRLFAAPAHPYTQALMSAVPVPDPTRRRAHTKVRGEPPSPEQET
                     RQTWLIVMSLLVCSVGICNSMFMSVTERFREIGTMKCLGAVDAFVVKLFLLESGMLGL
                     LGSLIGAIAGFLVVCVVHLLKRQLGQIASMDWAYFGMGLVEALVIGMLLSMAATIAPA
                     VKAARLPPAAALRTDV"
     gene            621..1373
                     /locus_tag="PLQ54_20045"
     CDS             621..1373
                     /locus_tag="PLQ54_20045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOS95613.1"
                     /translation="MLEDYVVRTRGLTKIYVMGGEELHALDNVALDIKRGEYISIMGP
                     SGSGKSTLFNMIGGLDKPTSGTVYINETDMAQLDAAELAWIRCRTVGYIFQTFNLIPV
                     MTAIENVTLPMVFAGRTPDEQLQRGKELLELVGLGDRLYHRPSELSGGQQQRVAIARA
                     FANDPAIILADEPTANLDLRTGKEIIELLRTLNEERGVTVISATHDLHMIDISDRVVW
                     VRDGKIDKIEDRQSVDVKVGHVDWEDQYRPTN"
BASE COUNT          259 a          467 c          480 g          248 t
ORIGIN      
        1 tcgaccgcct gttcgccgcc cccgcccatc cctacaccca ggcgctgatg tcggccgtgc
       61 ccgtccccga cccgacccgc cggcgcgcgc acaccaaggt ccgcggcgag ccccccagcc
      121 ccgagcagga gacccggcag acgtggctca tcgtcatgtc gctgctcgtg tgcagtgtcg
      181 ggatctgcaa ctcgatgttc atgtcggtca ccgagcggtt ccgggagatc ggaacgatga
      241 agtgccttgg ggccgtggat gcattcgtgg tgaagctgtt cctcctcgag tccggcatgt
      301 tgggtctcct gggctctctc atcggggcga tagcgggttt cctggtcgtc tgtgtggttc
      361 atctgctcaa acgccagctc ggccagatcg cgagtatgga ctgggcctac tttggtatgg
      421 gattggtcga ggcactggtg atcggcatgc tcctctcgat ggccgccacc atcgcgccgg
      481 ccgtgaaggc cgcccggctg ccgcctgcgg ccgcattgcg tacggacgta tagccccgcg
      541 gggcccccgc gggtcgagag tggacatccg tgtgacggag gttctcgatg gcaacgagcg
      601 ctgcggcgaa ccatagccag atgctggaag actatgtggt ccggacccgc ggactcacca
      661 agatctacgt tatgggcggt gaggaactcc atgccctgga caacgtagcg ctcgatatca
      721 agcggggcga gtacatttcg atcatggggc cgtcaggctc cggcaagtcg acgctgttca
      781 acatgatcgg cggcctggat aagcccacat ccgggacggt gtacatcaac gaaaccgaca
      841 tggcgcagct cgacgcggcg gagctggctt ggatccgttg ccggacggtc gggtacatct
      901 tccagacctt caacctgatc ccggttatga cggccatcga gaacgtcacg ctcccgatgg
      961 tcttcgccgg acgcacaccc gacgagcagc ttcagcgcgg caaggagctt ctcgagctcg
     1021 tggggttggg cgaccgcctg taccaccgcc cctcggagct ctcgggaggc cagcagcagc
     1081 gcgtggccat cgcccgagcg ttcgccaacg accccgccat catcctggcc gatgagccca
     1141 cggccaacct cgacctgcgc accggcaagg agatcatcga gctgctccgg acgctgaacg
     1201 aggaacgggg cgtgaccgtg atctcggcca cacacgacct gcacatgatc gacatctcgg
     1261 accgcgttgt ctgggtgcga gacggcaaga tcgacaagat cgaggaccgg cagtcggtgg
     1321 acgtgaaagt gggccatgtg gactgggagg accagtaccg gcccacgaac tagcggagcg
     1381 gcgagcacgc gtggatgaga gccagcaggg cctgccaggg ccggttgcaa tgccggaaac
     1441 ctcgggggtg gacc
//
LOCUS       DAOSPL010001392         1452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_88784_length_1452_cov_3.245526, whole genome shotgun sequence.
ACCESSION   DAOSPL010001392 DAOSPL010000000
VERSION     DAOSPL010001392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88784_length_1452_cov_3.245526"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PLQ54_20050"
     CDS             complement(<1..918)
                     /locus_tag="PLQ54_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOS95614.1"
                     /translation="MTFRLACFIGITATACAPLAAQAPGALNVRDFGAVGDGQADDTA
                     AFQSTLDQCAAAGGGIVYAPTGRYRIATHLTIPRAVTLEGVFTAPPTVNAYHDPADPA
                     AAPLLGGTVLLAEEGAGDENGPAFIGLDTNATLNGVTIFYPRQTKTNPPVAYPWAVKC
                     IGADNCAVIDVLMVNPYQAVDFGSAPSGRHMIRNLYAQALRRGVYIDRCLDVGRVENV
                     HIWPFWTAADADSPVATFMLEQGEAFILGRSDWELMTNCFAIAYNTGFRFTANPDQGP
                     HGGAGNYMITGGGADMCRSALLVEQTQGHS"
BASE COUNT          222 a          504 c          508 g          218 t
ORIGIN      
        1 cgagtgcccc tgggtctgct ccacgagcag cgccgagcgg cacatgtcgg ccccgccgcc
       61 ggtgatcatg tagttccccg cgccgccgtg agggccctgg tccgggttgg cggtgaatcg
      121 gaaccccgtg ttgtaggcga tggcgaagca gttggtcatg agctcccagt ccgatcggcc
      181 caggatgaac gcctcgccct gctcgagcat gaacgtcgcc accgggctat cggcatcggc
      241 cgccgtccag aagggccaga tgtgcacgtt ctcgacccga cccacatcga ggcaccggtc
      301 gatgtagaca ccgcgcctca gtgcctgggc gtacaggttg cggatcatgt gccggcccga
      361 cggcgccgag ccgaagtcca ccgcctggta ggggttcacc atcagcacat cgatgacggc
      421 gcagttgtcg gcgccgatgc acttcaccgc ccacgggtag gccaccggcg gattcgtctt
      481 cgtctggcgg gggtagaaga tagtcacacc attgagcgtg gcgttggtgt cgagtccgat
      541 gaacgcgggc ccgttctcat cgcccgcgcc ctcctcggcg agcagcacag tccccccaag
      601 cagcggcgcc gcggccgggt cagcgggatc gtggtaggcg ttcaccgtgg gcggcgcggt
      661 gaagacgccc tcgagtgtca cggctctcgg aatcgtcagg tgtgtggcga tgcggtagcg
      721 cccggtgggc gcatacacga tgccaccccc cgccgcggca cactgatcca gcgtggattg
      781 gaaggcggct gtgtcgtcgg cctggccatc gccgacagcg ccgaagtcgc gcacgttcag
      841 cgcccccggc gcttgcgccg ccagcggggc gcacgccgtt gctgtgatac cgatgaagca
      901 tgcgaggcgg aaggtcatgg gggtacctcc tgagggccgg atgccgtcga ggcgagtggt
      961 tcgccggcgg catcgcggac accggccgca ggtgctggga gggggcacca gcccggcgca
     1021 tgtcgtcgaa ggcctccgac cgggtcgatg cgcggcgacg gcgagccaga ccgcccacca
     1081 cgcaccaagc gcccgtcccg cgcatcgccg atgaccgccc acgtcgagaa cggacgatcc
     1141 gggtagtctg atgcggccac gccccagtac ttgccatcgg ccgtgagcga catgcggagg
     1201 ccggcctgct ccaatctggc cgccacgccc gggccgtcgc tcggcagcta ccctcggagt
     1261 tgagcccagt agtcgggtct gatcgtcggc ctcaccgcgc gaccgcagca ccgatcccgg
     1321 cgcggtgtgc cttgagccag agcgccactc ctgtgcccac cgtcgcgcag atggcggcgt
     1381 tgagacacac cgctcgaggc gcgccctgcc agttggccag tgcgccgctg aggaagctgc
     1441 cgatggggcc ga
//
LOCUS       DAOSPL010001393         1452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_88810_length_1452_cov_2.422334, whole genome shotgun sequence.
ACCESSION   DAOSPL010001393 DAOSPL010000000
VERSION     DAOSPL010001393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88810_length_1452_cov_2.422334"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLQ54_20055"
     CDS             <1..251
                     /locus_tag="PLQ54_20055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium transporter"
                     /protein_id="HOS95615.1"
                     /translation="ALPGIPFLNRTGIAFWVCMGASAVVSLLTRPKPAEELEGLIWNR
                     DSLKLPPEMRERMRGVRNPAVWWAAITAVVVAMYVVYR"
     gene            346..>1452
                     /locus_tag="PLQ54_20060"
     CDS             346..>1452
                     /locus_tag="PLQ54_20060"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOS95616.1"
                     /translation="MWLPLVFLMAAAERPAWLASTVYDDYGRDGRVLKPWTPLAYGPR
                     EVSVWGRTFRWAPDSVLPSRIESQGTALLLAPMELRLRVGGKNHTVPLERFRFTQRKP
                     NRGSLEAVGSVAGVRFRADMWIEYDGFLWVTLRVDDAPAEPIESLHIVATMPKPCATL
                     YQTFCRPLTGWVGDKPIALLWFSNRAEKIVDFYHWLGNEDLGLGFTYSTLEHWAPASE
                     DEFCTIEPGADAVRYRLNLIERPSPLSGRAFTFGIQPTPIKPLPPDYHSMINSTWNTK
                     SWAAWRNMADDVDMAIVWPPPQMRGLNDPYHVKPEPLREAVADAHKLGVAVLFTGCPQ
                     KVSPLTDELEQYRGEWLVQPESILDWEGVPHYQNC"
BASE COUNT          222 a          492 c          497 g          241 t
ORIGIN      
        1 aggcgctgcc gggcattccg ttcctgaacc ggaccggcat cgcgttctgg gtctgcatgg
       61 gggcgtcggc cgtcgtgagt ctgctgaccc ggcccaagcc ggcagaagag ctcgaagggc
      121 tcatttggaa tcgcgacagc ctcaagctgc cgccggagat gcgcgagcgc atgcgcggtg
      181 tccgcaaccc ggccgtctgg tgggcggcga tcactgccgt ggtcgtcgcc atgtacgtgg
      241 tgtaccgcta ggaggagtgc gcgccgacgg cagggtgcgc cgcgtcgtag ggggtcgtga
      301 ccgttccatg gcccgagctt cagggaggct aatggcttgc atgccgtgtg gttgccgctc
      361 gtgttcctga tggccgcggc tgagcggccg gcgtggctgg cgagcaccgt ctatgacgac
      421 tacggccgcg acgggcgcgt cctcaagccg tggacgccgc tcgcctacgg gccgcgggag
      481 gtctcggtct ggggccggac gttccgctgg gcgcctgaca gcgtgctccc gagccgcatc
      541 gagagccagg gcacggcgct gctcttggcg ccgatggagc tgcggctgcg cgttggcggc
      601 aagaaccaca ccgttccgct cgagcggttc cggttcaccc agcggaagcc gaaccgcggc
      661 tcgttggagg ccgtcggctc ggtcgcgggc gtccgcttcc gtgccgacat gtggatcgag
      721 tacgacggct tcctctgggt cacactgcgc gtcgacgatg cgccggccga gcccatcgag
      781 tccctgcaca tcgtcgccac gatgccgaag ccgtgcgcca cgctctacca gaccttctgc
      841 cggccactga ccgggtgggt gggcgacaag cccatcgccc tgctgtggtt ctcgaaccgg
      901 gccgagaaga tcgtcgactt ctaccactgg ctgggaaacg aggatctggg cctcggcttc
      961 acctacagca ccctcgaaca ctgggcaccc gcatcggagg acgagttctg caccatcgag
     1021 cccggcgccg atgccgttcg ctaccgcctg aacctgatcg agaggccctc gccgctatcg
     1081 ggcagggcct tcaccttcgg catccagccc acgcccatca agccgctgcc gccggactac
     1141 cactcgatga tcaacagcac gtggaacacg aagagctggg cggcttggcg taacatggcc
     1201 gatgatgtcg acatggccat cgtctggcct cctccgcaga tgcgcgggct caatgacccc
     1261 taccacgtga agcccgagcc cctgcgtgag gcggtggccg acgcccacaa gctcggcgtg
     1321 gccgtgttgt tcaccggctg cccgcagaag gtcagcccgc tgactgacga gctcgagcag
     1381 taccggggcg agtggctcgt ccagccggag tcgatcctcg actgggaggg cgtgccgcac
     1441 taccagaact gc
//
LOCUS       DAOSPL010001394         1451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_88879_length_1451_cov_3.417622, whole genome shotgun sequence.
ACCESSION   DAOSPL010001394 DAOSPL010000000
VERSION     DAOSPL010001394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88879_length_1451_cov_3.417622"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PLQ54_20065"
     CDS             <1..420
                     /locus_tag="PLQ54_20065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95617.1"
                     /translation="WFPGDVRVLLDCSPDAVGTPGEDSFWMHLHFDFLGTTGWMHLTQ
                     NRGYWYQAHGMAEPVRGASSWDLQGQAGQRDFTQAVADWLDGGPPHLNRFAVGRAVVE
                     ALFAAQKSALLGKRISLPTTFTDAEWQALRDRLGASA"
     gene            complement(493..>1451)
                     /locus_tag="PLQ54_20070"
     CDS             complement(493..>1451)
                     /locus_tag="PLQ54_20070"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOS95618.1"
                     /translation="TVRAVDAPYRRPRLGKRRCTMREVRWERMFPDQLEEAFAECPVL
                     YLPYGLCEPHGPQCALGLDALKAHAICCEAARAHGGIVAPADYWHIHELGGYAIWARK
                     FVGEPPHTWATAMPPWQHFRNVLYHIRTADVHGFKAALLVTGHYGPNWQDLCRLCELV
                     QPRVGTRLYALPDFEANVPGFDSDNRSGGDHAGKIETSLLAAVYPECVDMTRIPPADQ
                     AGTHFAMGPDVREASRLVGERMVADEVRYLGARARELLNEYDRVRPAHSLRTFEQVEQ
                     LWDAVVQPVFHTFLTMQDAWGEHEPVPSESIWHDNWRVAKRG"
BASE COUNT          229 a          505 c          483 g          234 t
ORIGIN      
        1 tggttccccg gggatgttcg cgttctgctc gactgcagcc ccgatgcggt gggcaccccc
       61 ggcgaggact ccttctggat gcaccttcac ttcgacttct tgggcaccac aggctggatg
      121 catctgaccc agaaccgcgg gtactggtac caggcccacg gcatggcgga gcctgtgcgg
      181 ggtgccagca gctgggacct tcagggccaa gcagggcagc gcgacttcac ccaggcggtg
      241 gccgactggc tcgacggcgg cccgccgcac ctgaaccgtt tcgccgtggg ccgcgcggtc
      301 gtcgaggcgc tctttgcggc acagaagtcg gctctcttgg ggaagaggat cagcctgccc
      361 acgaccttca ccgacgccga gtggcaggcc ctgcgcgacc gtctgggcgc ttcggcgtag
      421 gggcggggcc tgctccgccc cgtcgctcta atgcgccggg ccagagcgcg cgacgacggt
      481 gccaggaccg gcctatccgc gcttcgccac tcgccagttg tcatgccaga tgctctcgct
      541 cgggacgggc tcatgctcgc cccacgcgtc ctgcatggtg aggaacgtgt ggaagaccgg
      601 ctgcaccacg gcatcccaca gctgctccac ctgctcaaag gtccggagcg aatgagccgg
      661 gcgcactcgg tcgtactcgt tcagcagctc gcgggccctg gcgccgagat agcggacctc
      721 gtcggccacc atccgctcgc ccacgagccg actggcctca cggacgtcgg gccccattgc
      781 gaagtgggtc ccggcttgat ccgcaggggg gatgcgagtc atgtccacgc actccgggta
      841 gaccgcggcg agcagagagg tctcaatctt gcccgcgtga tcgcccccgc ttcggttgtc
      901 gctgtcgaag ccaggcacgt tagcctcgaa gtccggaagc gcgtacagcc gagtccccac
      961 ccgcggctgg accagctcac acaggcggca gaggtcctgc cagttcggcc cgtaatgacc
     1021 ggtgaccagc agagccgcct tgaagccatg cacgtccgcg gtccggatgt gatacaggac
     1081 gttgcggaag tgctgccacg ggggcatggc cgtggcccag gtgtgcggcg gctcgcccac
     1141 gaacttgcgg gcccagatgg cgtaaccacc cagctcgtgg atgtgccagt agtcggccgg
     1201 ggcaacgatg cctccatggg ctcgcgcggc ctcacagcag atggcgtgcg ccttgagtgc
     1261 atcgagtccc agggcgcact gcggcccatg gggctcgcac agcccatacg gcaagtagag
     1321 caccggacac tccgcaaacg cctcttcgag ttggtcgggg aacatccgtt cccaccgcac
     1381 ctcgcgcatg gtgcacctcc gcttcccaag acgtggtcga cggtatgggg cgtccactgc
     1441 ccgcacggtc a
//
LOCUS       DAOSPL010001395         1447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_89234_length_1447_cov_3.410201, whole genome shotgun sequence.
ACCESSION   DAOSPL010001395 DAOSPL010000000
VERSION     DAOSPL010001395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89234_length_1447_cov_3.410201"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLQ54_20075"
     CDS             <1..594
                     /locus_tag="PLQ54_20075"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOS95619.1"
                     /translation="LGDWVLPVLAEPAAYGAGPLWLALYGYAAYIYINFAGVSDIAIG
                     LGAAFGVAVPENFARPYLQLDIQSFWRSWHITLTRWLQAYIFMPISKRLMRTRLRRHP
                     KLVASVGYLVTFAFCGVWHGDTAGLLLWGLYHGLGLCIYAVLPVSLKVLPHSRPSPLR
                     RLVWWTITFHFVTLGWLLFACPLPTALRALARLVGFG"
     gene            complement(1326..1447)
                     /locus_tag="PLQ54_20080"
                     /pseudo
     CDS             complement(1326..1447)
                     /locus_tag="PLQ54_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          191 a          498 c          460 g          298 t
ORIGIN      
        1 ctgggcgatt gggttctgcc agtcctggct gagcccgcgg cctacggcgc cgggcccctg
       61 tggctcgcgc tctatggcta cgccgcctat atctacatca acttcgccgg cgtctcggat
      121 atcgccatcg ggctcggtgc cgccttcggc gtagccgtgc ccgagaactt cgcccgcccc
      181 tatctgcagc tcgacatcca gtccttctgg cggagctggc acatcacact cacgcgttgg
      241 ctacaggcat acatcttcat gccgatctcg aagcggctca tgcgaactcg actccgccgt
      301 catccgaagc tcgtggcgtc ggtcggctac ctggtcacgt tcgccttctg cggcgtgtgg
      361 catggcgata ccgcgggtct actgctctgg gggctctacc acgggctcgg gctatgcatc
      421 tacgccgtgc tgcccgtatc gctgaaagtc cttcctcact cccggccctc tccgctgcgt
      481 cggctcgtat ggtggaccat caccttccac ttcgtcaccc tcggctggct gctgttcgcg
      541 tgtccgctcc cgacggcgct gcgcgcgctc gcccgcctgg ttgggttcgg ctagacccag
      601 cccgatgcga ggaggaagag gcatggccga gtgatgcaga gaagcgcgac gaggtcgccc
      661 acaaggccga cgaggtcgaa cggcgatggg cgagagactg aggcgcggcc ccgtgcggtc
      721 cgggggcccg cgtctggtcc tggcggctgc cgtccgtgtc ttgacagcca gccgggtcga
      781 gtcgatgatg gacttatcat catggatatg gaggcgtggc ccggttgcgt ccagtcgggt
      841 cggatgacgg ccgaccggaa acctacgtcc ctcctcgcct gatcccctac aacctgcgca
      901 gttccctgcg ccgggtgaca ctctcgtggg cgtttggctc gctgtggatg tacatcacca
      961 ccggcgccgc gctcactcgc tacgcgaaac tactgcacat gcctgagtac ggcttcggcc
     1021 tgttggctgc cgtgccgttc gtcagtgccc ttgcccagct accggccagc tacgtgatgg
     1081 agcactacgg aggccgcaaa cgcctgttcc tggccgccgg gttgttccat cggtcgatgt
     1141 gggtgctcgt ggccgccatc ccgtggttca tccctgaggc ctggtggtgg atgtccctgc
     1201 tcggcctgct ggccatctca tccctggcgg gacatgtggc gacacccggc gtctacggtt
     1261 ggttcgccga tctggtgccg cgccaggtgc gcggaaggta cttctcccgc cgcatgcagg
     1321 tgggccagct tgtcggcctc gccgaacgga atctccatcg cgcgcgcgat gtcacggatc
     1381 acgccgcgcg ccttgagctg gtggaacgtg gcgatctggc cgacctgctc gcgcccgtac
     1441 ttctcct
//
LOCUS       DAOSPL010001396         1444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_89510_length_1444_cov_3.055436, whole genome shotgun sequence.
ACCESSION   DAOSPL010001396 DAOSPL010000000
VERSION     DAOSPL010001396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89510_length_1444_cov_3.055436"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PLQ54_20085"
     CDS             complement(<1..673)
                     /locus_tag="PLQ54_20085"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95620.1"
                     /translation="MGAEALGESAGSLDIGSNTVRVLVARMVGGRPVRILDTGAFARL
                     ADGLAETGRLSDQRMARALELVREYSEAARRSGARRLYPFCTAAVRQASNGTEFAQRV
                     SEAAGAPARVLTGQEEACWAFVGATQALSVDGEAVVLDIGGGSTELVMGRGGRVRYAE
                     SYLVGSRSLGQALPHCLGPLDELGVERLVASAGGVLDDHGVPRCRQGDGVVIGTGGTF
                     TVLAAL"
     gene            complement(663..1016)
                     /locus_tag="PLQ54_20090"
     CDS             complement(663..1016)
                     /locus_tag="PLQ54_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOS95621.1"
                     /translation="MRTVHYTSVASESVGPEASGVSVRWVISDADGAPGFALRVFEIE
                     PGGYTPRHQHPWEHEVFVLAGEGCVRSASGETRLSEGTAVLVEPDELHQFANPGGVPF
                     RFICVVPNAAYGHGR"
     gene            1171..>1444
                     /locus_tag="PLQ54_20095"
     CDS             1171..>1444
                     /locus_tag="PLQ54_20095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOS95622.1"
                     /translation="MQSDTPGRRMTRRDFVRRSAATAVLGGMLAAPSIPAAAQEVAPP
                     GGIPRRRIPKLDTDVSVVCFGSFGLTNPAVLEAALDAGVNLIDTGPG"
BASE COUNT          242 a          586 c          426 g          190 t
ORIGIN      
        1 ccagtgcggc cagcaccgtg aatgtccccc cggtgccgat cacgactcca tcgccctgcc
       61 ggcaacgcgg cactccgtga tcatcgagta caccaccggc cgacgccacg agtcgctcga
      121 cgcccagttc atcgagtggc cccaggcaat ggggtagggc ctggccaagg ctgcgcgatc
      181 ccaccagata gctctcagcg taccgcacac ggccaccccg gcccatcacg agctccgtac
      241 tgcccccacc gatgtcgagc accacagcct ctccatcaac agacaaggcc tgggtcgccc
      301 ccacgaaggc ccagcacgcc tcctcctgcc ccgtcagcac gcgcgccggt gccccggcgg
      361 cctcggatac ccgctgggca aactccgtgc cattcgacgc ctggcgcacc gccgccgtgc
      421 agaacgggta caaccggcgc gcacccgatc gtcgcgccgc ctccgagtac tcgcgcacca
      481 actcgagggc tcgggccatc cgctggtccg acagccggcc ggtctcggca agaccatcag
      541 cgagacgggc gaaggccccg gtgtccagaa tccggaccgg ccgaccgccc accatccgcg
      601 ccacgagaac ccggaccgtg ttcgacccga tgtccagcga accggccgac tcgcccagcg
      661 cctcagcgcc catgaccgta ggccgcattg gggacgacgc agatgaaccg gaacggtacc
      721 ccgccggggt tggcgaactg gtggagctcg tcaggctcca ccagcacggc cgtgccctcg
      781 ctcaagcgcg tctcacccga agcggagcga acacagccct cgccggccag cacgaacacc
      841 tcgtgctccc acgggtgctg atgccttggc gtatagcccc cgggctcgat ctcaaacacg
      901 cgcagggcga acccgggagc gccgtcggca tcagagatga cccatcgcac gctcacccca
      961 ctggcctcgg gccccaccga ctcgctcgcc acgctcgtgt agtggaccgt cctcatgcgt
     1021 cccacccctg gcggccgccg ttcgcccgca gctggtgtca gtccttctgg cggcagggcc
     1081 acgagggttt gaaccggcgt gtggcgaacc agactcgaca ggtgcactcg cccgcgccgt
     1141 caccgaccgg tgcgacctgg agggtccacg atgcaaagcg acacgcccgg ccggcgaatg
     1201 actcgtcgcg acttcgtccg tcggtccgcc gccaccgccg tgctgggggg catgctcgcc
     1261 gccccgagca ttcccgccgc cgcccaggaa gtcgccccgc ccggcggcat cccgcgccgc
     1321 cgcatcccca aactcgacac cgacgtgtcg gtcgtatgct ttggctcctt cggcctcacc
     1381 aatccggcag tcctcgaagc cgcactcgat gccggggtca acctcatcga caccggaccc
     1441 ggct
//
LOCUS       DAOSPL010001397         1444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_89548_length_1444_cov_2.109431, whole genome shotgun sequence.
ACCESSION   DAOSPL010001397 DAOSPL010000000
VERSION     DAOSPL010001397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89548_length_1444_cov_2.109431"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PLQ54_20100"
     CDS             complement(<1..662)
                     /locus_tag="PLQ54_20100"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOS95623.1"
                     /translation="MSGTGEPGNREIRRARIARGNTLGGAGSPFELSAADARLQQEHF
                     GVAPDQILHDFVISHVLAALEPLSDRFVFYGGTALSRTLLNGLRLSEDIDLLSTGPRA
                     EPARLIDSALSGLQRGLGSVSADVRLQDVTTDTAPCTYRIGEVPVRIQLISAAHYPTW
                     PTQRSMVAQRYSGLPDLELQTYTAEGFVAAKTVAWCDSTRNAPRDLYDLWALAERGFV
                     TAG"
     gene            complement(659..1303)
                     /locus_tag="PLQ54_20105"
     CDS             complement(659..1303)
                     /locus_tag="PLQ54_20105"
                     /inference="COORDINATES: protein motif:HMM:NF020962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HOS95624.1"
                     /translation="MARHLVPKRLPAAPLRTVRPLMLRDVYTNPEKELVRLRRQGLVV
                     RIAPGTYTVKPDTVAPEDNWRPPFEHAAMAYATAQYGDRVPVLYGIGAARFHHAIPRA
                     IAVAVVAVPEQHRPVTLTDGGKVVFTTTDVDALDARLERVPIGAFLVTTPEQTLIDLV
                     ARPQLGGMPGEAGAAARALATTTDRDRVIELLAARPGTVQRGVRTLLDDARGHQ"
BASE COUNT          211 a          430 c          550 g          253 t
ORIGIN      
        1 cctgcggtga cgaaaccgcg ctcggccagg gcccacaggt cgtataggtc gcggggcgca
       61 ttcctggtgc tgtcgcacca ggcgacggtc ttggccgcta cgaaaccttc ggctgtgtag
      121 gtctggagtt cgaggtcggg gaggcctgag tagcgctgcg ccaccatgct tcgctgagtg
      181 ggccaggtgg ggtagtgcgc tgcactgatg agctggatcc tcacagggac ttccccgatc
      241 cggtaggtgc agggcgccgt gtcggtcgtg acgtcctgga ggcgtacgtc ggccgacacg
      301 cttccgagtc ctcgttgcag cccggagagg gctgagtcga tgagccgggc gggttcggcg
      361 cgcggcccgg tcgacaggag gtcgatgtct tcgctgaggc gcaggccgtt cagcagtgtg
      421 cggctgagtg ctgtgcctcc atagaacacg aacctgtcgg agaggggctc gagtgcagcg
      481 aggacgtgcg agatgacgaa gtcgtgcagg atctgatccg gggcgacacc gaagtgctcc
      541 tgctggaggc gagcgtcggc cgctgacagc tcgaaggggg agccggcgcc gcccagggtg
      601 ttgcctcgcg caatccgcgc tcgccgtatc tcgcggttcc ccggctcgcc agttccgctc
      661 attggtgtcc ccgtgcgtcg tcgagaagcg tgcggacccc gcgctggaca gtgccggggc
      721 gagcagcgag tagctcgatg acgcggtcgc gatcggtggt ggtggcgagt gctcgggcgg
      781 ccgcgccagc ttcccctggc atgccgccaa gctggggtct ggcgaccagg tcgataagag
      841 tctgttccgg ggtggtgacg aggaaggcgc cgatcgggac gcgttcgagg cgtgcgtcca
      901 gggcatcgac gtcggtggtg gtgaagacga ccttgcctcc gtccgtgagg gtgacgggac
      961 ggtgctgttc ggggacggcg acgacggcga cggcgatggc acgcgggatg gcgtggtgga
     1021 accgggcggc tccgatgccg tacagcacag gcacccggtc gccgtactgg gcggtggcat
     1081 acgccatggc tgcgtgctcg aaagggggcc gccagttgtc ctcaggggcc acggtgtcgg
     1141 gcttgaccgt gtaggtcccg ggcgcgatcc gtaccaccag cccctggcgg cgcagccgga
     1201 ccagctcctt ctcgggattg gtgtagacat cgcgaagcat caacggtcgg accgtacgca
     1261 gcggtgcggc cggcagtcgc ttcggcacca ggtgtcgcgc catgcttccc tcctcgaatg
     1321 cgcaaaacgc tacctgtcag ggagtgtttt gcacagtcca tggctctcaa gcggctgcgc
     1381 tcggccggat cttctgtgac gtagtcgcca gcggaccggt gagtgccagt tggcgaggag
     1441 tcgc
//
LOCUS       DAOSPL010001398         1442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_89668_length_1442_cov_3.333093, whole genome shotgun sequence.
ACCESSION   DAOSPL010001398 DAOSPL010000000
VERSION     DAOSPL010001398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89668_length_1442_cov_3.333093"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1442)
                     /locus_tag="PLQ54_20110"
     CDS             complement(<1..>1442)
                     /locus_tag="PLQ54_20110"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOS95625.1"
                     /translation="GASVLFLYSSLSRIVRMQLSVIGTLKALGFGNRALLAQYVAQGL
                     LISWAGALPGALLGHGLSILLAEAYADSLNLSIVKAPFHWDTLGASLALAGLTGLAGA
                     LGPARAAARLRPAVAMRGDVEPDGPRGRRNRLMEATRCLPVAYRIPVRGLFRRVSRTL
                     LAVAGISGGATITLTTLGMGVSTLDAIDEALNRTRLVELDVGYATLGPGAIATAISGL
                     PGTRAVSHTVSLPVRLRSASGEGKVLLMGVEPGQDMLRITAAGGGRMVPRKGQIWLPR
                     RLGERLAVDPGDPLRVQWTGASRRRPDGRTLRVAGFIDLAIGGVAYGHYEDIRRGLAD
                     RVYPYSSYGSMVDCVPGAVSPLRHRLERSDDVAIVYTRRDFQQQVNQQMGIFYIFMAV
                     LLGFGAVLSGCAIQSVASVSLLERTRELATLRSLGFSARATAGLAALELYALAALGLV
                     VGLPIGAHLNALYIQSYSTDTMTFHPLLPP"
BASE COUNT          256 a          461 c          542 g          183 t
ORIGIN      
        1 agggtgggag caggggatgg aaggtcatgg tgtcggtgga gtagctctgg atgtagagcg
       61 cgttgaggtg agcgccgatg gggaggccca cgaccagccc gagggccgcc agggcgtaga
      121 gctcgagggc agccaggccg gcggtcgccc gggccgagaa gcccaggctg cgcagtgtgg
      181 cgagctcgcg ggtccgttcg aggagcgaga ccgacgcgac gctctggatg gcgcaccccg
      241 agagcacggc accgaagccg agcaacacgg ccatgaagat gtagaatatg cccatctgct
      301 ggttgacctg ctgctggaag tcccggcggg tatagacgat ggcgacgtcg tcactgcgct
      361 cgaggcggtg tcgcagaggc gagacagcgc ctggcacgca gtcgaccata gacccatagg
      421 agctataggg gtagacgcgg tcggccaggc cgcgccggat gtcctcgtag tgcccatagg
      481 ccacgccgcc gatggcgagg tcgatgaacc cggcgacgcg gagcgtgcgg ccatcgggcc
      541 gccggcggct ggcccccgtc cactggactc ggagtggatc gcccgggtcc acggccaagc
      601 gttcgccgag acgcctcggg agccagatct gacccttgcg gggcaccatc cggccgcccc
      661 ccgcagccgt gatgcggagc atgtcctgcc cgggctcgac gcccatcagc agcaccttgc
      721 cctcaccgga agcggaccgc agtcggaccg gcaggctcac ggtgtgactg acggcacggg
      781 tccctggcag gccagagatg gccgtggcta tagcaccggg ccccaaagtg gcgtagccga
      841 cgtcgagctc gaccaagcgg gtgcgattga gggcctcatc gatggcatcg agagtcgata
      901 cgcccatgcc cagggtcgtc agcgtgatgg tggcacctcc cgagatgcct gccacggcca
      961 gcagcgtgcg tgagacgcgg cggaacaatc cgcgtaccgg gatgcggtag gcgaccggta
     1021 ggcaacgggt cgcttccatc agccggttgc ggcggccgcg aggaccgtcg ggctcaacat
     1081 cgccccgcat ggcaaccgcc gggcggaggc gcgcggcggc gcgagccggc ccgagggcac
     1141 cggcgaggcc ggtgagcccg gccagcgcga gcgaggcacc gagggtgtcc cagtggaacg
     1201 gcgctttcac gatgctcagg ttgagcgaat cggcgtaggc ctcggccagg aggatggaca
     1261 gcccatggcc cagcagcgcg cccgggagag cgcccgccca gctgatcagc aggccctggg
     1321 ccacatactg cgccagtagg gccctgttcc cgaaccccaa ggctttgagg gtgccgatga
     1381 cgctcagctg catgcggacg atgcgggaga gtgagctgta gaggaagagg accgaagccc
     1441 cc
//
LOCUS       DAOSPL010001399         1440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_89864_length_1440_cov_2.521300, whole genome shotgun sequence.
ACCESSION   DAOSPL010001399 DAOSPL010000000
VERSION     DAOSPL010001399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89864_length_1440_cov_2.521300"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PLQ54_20115"
     CDS             complement(<1..360)
                     /locus_tag="PLQ54_20115"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOS95626.1"
                     /translation="MADDWWRSFFDDWYLDNWVGAAASAQVTAEQVDFIVQTLQLAPG
                     ARVLDACCGEGRHCRELARRGFDVVGVDQSAASLVRGQAQEPVVRYVHQDVREMRFDA
                     EFDAAINMFTAFGYMDEA"
     gene            complement(364..>1440)
                     /locus_tag="PLQ54_20120"
     CDS             complement(364..>1440)
                     /locus_tag="PLQ54_20120"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HOS95627.1"
                     /translation="PKVSGEGAKWSIVEAALARGDRRWGEVLCRVSETDGTYADYVRA
                     ARLSGIDLVAEATRARGRDEALPWEIIDTGVAREVLWRRWQRAWGGPPGDAPCAGRGP
                     SVPSVGEGPSLLRYDLGGAEVVVTCRRGGVSEGPYASLNLSLAVLDDDACVIENRRRV
                     IEALGGPVAFARQVHGTDVLRVDPGVSCLESSLGEADAMVTSAEGPALAIQVADCQAV
                     LVHDLRTGARGAAHSGWRGAAAGMALRLLEAMGRHFGTRPEDCAAFLSPCIGPCCYYI
                     PQEAEHPLKQPPGSGRYLLKPDGDGHALDLTAENAAQLAEAGVPRRGIMEAGLCTSCR
                     PDLFYSHVRDGARTGRMWVILRGR"
BASE COUNT          235 a          550 c          467 g          188 t
ORIGIN      
        1 ggcctcatcc atgtagccaa aggcggtgaa catgttgatc gcggcgtcga actcggcgtc
       61 gaaccgcatc tcgcggacgt cctggtggac gtagcgcacc acgggctcct gagcctgccc
      121 gcgaacgaga ctcgccgcgg actggtcgac accgacgacg tcgaagccgc gccgggcgag
      181 ttcccggcag tgccggccct ctccgcagca ggcgtccaga acccgcgcgc cgggcgccag
      241 ctgcagggtc tggacgatga agtccacctg ctcggccgtc acttgcgccg aagcggccgc
      301 gcccacccag ttatccaggt accagtcgtc gaagaaggac ctccaccagt cgtctgccat
      361 cggctacctc ccccgcaaga tgacccacat gcggcccgtg cgagcgccat cgcgaacgtg
      421 cgagtagaac aggtcgggcc ggcacgaggt gcacagcccg gcctccatga tgccgcggcg
      481 cgggaccccc gcttccgcca gctgggcagc gttctccgcc gtcagatcga gggcgtgtcc
      541 gtcgccgtca ggtttgagaa ggtatcgtcc ggagcccggc ggctgcttaa ggggatgctc
      601 ggcctcctgc gggatgtagt agcagcacgg cccgatgcag ggggagagga acgcggcgca
      661 gtcctcgggc cgggtgccaa agtgccgacc catcgcctcg agcaaccgga gtgccatgcc
      721 ggccgcggcg cccctccagc ccgagtgcgc ggccccgcga gctcctgtcc ggaggtcatg
      781 caccaggacc gcctggcaat cggccacctg gatggccagc gcgggcccct ccgccgaggt
      841 taccatcgcg tccgcctcgc cgagcgagga ctcgaggcat gacacacccg gatcgacccg
      901 cagcacatcg gtgccatgca cctgccgcgc gaacgcaacg ggtcccccca gggcctcgat
      961 gacccgcctc cggttctcga tgacacacgc gtcgtcgtcc aacaccgcga gcgagaggtt
     1021 caggctggcg taggggccct cagagacacc gccgcggcgg caggttacca ccacctcggc
     1081 cccacccagg tcgtaccgca acaggctcgg gccctcgccg accgacggaa cggagggacc
     1141 ccgacccgcg cagggggcgt caccgggcgg gcccccccac gcccgctgcc agcgccgcca
     1201 gagcacctcg cgagccactc ccgtgtcgat gatctcccac ggaagtgcct cgtcgcggcc
     1261 gcgcgcgcgg gttgcctcgg caaccaggtc gatgccacta agcctcgcgg ctcggacgta
     1321 gtcggcatag gtgccgtcgg tctcgctgac ccggcacaga acttcacccc agcgccgatc
     1381 accccgtgcc agcgcggcct ccacgatcga ccacttggca ccctcgcccg agaccttggg
//
LOCUS       DAOSPL010001400         1439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_89998_length_1439_cov_1.989884, whole genome shotgun sequence.
ACCESSION   DAOSPL010001400 DAOSPL010000000
VERSION     DAOSPL010001400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89998_length_1439_cov_1.989884"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PLQ54_20125"
     CDS             complement(<1..536)
                     /locus_tag="PLQ54_20125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95628.1"
                     /translation="MAAAGLRGKTLPWLTGVEGRGSMMRRSNMGLVVLFSCIAACAAG
                     VLCYPNVGAQGGPGRHILASGDVPEVLASWHRMRGGSTGVGTRELQPNVPFVVDVPKI
                     SPEVRGTAELADVGTPVEVSVEVRRIDNLEEANPVTRVQVVVFDAECNSEKTFSNDWG
                     PVTDYSIPGSTSHIEYPIA"
     gene            complement(682..1296)
                     /gene="coaE"
                     /locus_tag="PLQ54_20130"
     CDS             complement(682..1296)
                     /gene="coaE"
                     /locus_tag="PLQ54_20130"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOS95629.1"
                     /translation="MYVLGITGPIAAGKTTVMGLFVSLGAEALAADDLTRELMAPGSA
                     LVAAIADEFGASFLLSDGSLDRRALGRMVFADPDALSRLNRIVHPTLKDTIARRIHQA
                     RSRPEPPPVLVVEAAVMGEMGALDLLDGLLYVDADEATRVRRLVQRDGMDLEEATCRV
                     RAQEAVRHLAKKANWVMDGAQDLDALRAQCVTIYKAIGWSRPSS"
BASE COUNT          240 a          504 c          463 g          232 t
ORIGIN      
        1 gcaatggggt actcgatgtg gctggtgctc cctgggatgg agtaatcggt cacaggcccc
       61 cagtcgttcg agaaggtctt ctcgctgttg cactccgcgt cgaagaccac gacctgcacc
      121 cgggtgacgg ggttcgcctc ttccagattg tcgattcgcc gaacctcgac actgacctca
      181 accggagtgc ccacatcggc cagttcggcc gttccgcgga cctcggggct gatcttgggc
      241 acgtcgacca cgaaggggac attgggctgc agctcccgtg tgccgacacc ggtcgagcca
      301 ccgcgcatcc ggtgccagct ggcgagcact tcgggcacgt cacccgacgc caggatgtgc
      361 cgaccgggcc ccccttgtgc tcccacgttg gggtagcaca acaccccggc ggcgcacgcc
      421 gcgatgcagg agaacagcac tacgaggccc atgttcgatc tgcgcatcat cgatccacgc
      481 ccctcaactc cggtgagcca cgggagagtc ttgccccgca gcccggcggc agccacgcca
      541 aacagactcc gccatccggc tcgaccgccc cgcaggctcg ggcgcggcca cttcgatggt
      601 cgtccggccg ttcatcacct gggtggagcg acacgaaccg gcgatcgggc ccaagcgcca
      661 ccacgggcgc gcccggtcac cttatgaaga cggcctgctc cagccaatcg ccttgtatat
      721 cgtgacgcac tgggcccgta aggcgtccag gtcctgcgcg ccgtccatga cccagttggc
      781 tttcttcgca agatggcgaa cggcctcctg cgcgcgcacg cgacaagttg cctcctcgag
      841 gtccattccg tcgcgctgca cgaggcgacg gacgcgggtc gcctcgtcgg cgtcgacata
      901 cagcagccca tcaagcaggt cgagcgcgcc catctcgccc atgacggccg cctccacaac
      961 gagaaccggg ggaggctcgg gccgcgaccg agcctgatga atgcggcgtg ctatcgtgtc
     1021 tttgagggtc ggatggacga ttcggttcaa ccgggagagg gcgtcggggt cggcgaagac
     1081 catgcggccc agggctcgcc gatcgagtga gccgtcggat agcagaaaag acgcaccaaa
     1141 ctcgtcggcg atggcggcca cgagggcgga cccgggggcc atgagttccc gtgtcaggtc
     1201 gtcggcggca agggcctccg cgcccaacga aacgaagagc cccatcaccg tcgtcttacc
     1261 ggcagcgatg gggcccgtga tgccgagaac gtacactgcg atggctcgct gtccctaggc
     1321 gtcctccggc cgctcgccgg gcgcctcttc ctcctcgcgt gggccgactg cctgctggag
     1381 cagatgaccg aactggtccc cgatggtcac attgccgggc tcctggctgg ctgctgcct
//
LOCUS       DAOSPL010001401         1435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_90297_length_1435_cov_3.299275, whole genome shotgun sequence.
ACCESSION   DAOSPL010001401 DAOSPL010000000
VERSION     DAOSPL010001401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90297_length_1435_cov_3.299275"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="PLQ54_20135"
     CDS             <1..939
                     /locus_tag="PLQ54_20135"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOS95630.1"
                     /translation="PGDTVFVPEERLEVSVVGHVTRQGRYRLRPGDRISDALAAAGGP
                     LRPTTVPTQTTGADLEHCWLYRATGEKLPMDLRFLVEPGGTNGAEARDRTRAEIDAPA
                     NLPLEPNDTIVVPEADRRVKVAGYVKDPGYFEYRPGETVSAALAMAGGVLTNIGSDSR
                     VQLRWPSGAEEEVDLRQSDPPLTPGVEIRVPFARNRVVVLGYVEKAGFYEWHEGDTVI
                     DAIALAGGIKGPTTNVGMRLVKGEYYRALLIRRENGVEKRYDLDLKSYYERGDQTHNL
                     RVYPDDVIMVPGKGKFNPQALLQDVLLIPDFLNLFR"
     gene            976..>1435
                     /locus_tag="PLQ54_20140"
     CDS             976..>1435
                     /locus_tag="PLQ54_20140"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HOS95631.1"
                     /translation="MVVRSRAPVRIDFAGGWTDVAVFADSVGGAVVSATIDKYVHGHM
                     RTMDGGQRPADPAPDAPGAWEWQVPGEGITVAYHSDLPAGSGLGTSATLNVVWLSLVR
                     ARPVNEADRATLAELAYDLERILGILGGRQDQYASAFGGVNYLTFGGTTGV"
BASE COUNT          242 a          477 c          489 g          227 t
ORIGIN      
        1 cccggcgaca ccgtgttcgt tcccgaggaa cgcctcgagg tctcagtggt gggtcatgtg
       61 actcgccagg gccggtaccg cctccggccc ggcgaccgga tctcagacgc cctggcggcc
      121 gccggcgggc ctctgcgccc aacaacggtc cccacgcaga ccaccggcgc tgacctcgag
      181 cactgctggc tctaccgcgc cacaggagag aagctcccca tggacctgcg gttcctggtc
      241 gagcccggtg gcaccaatgg ggccgaggct cgcgaccgca cccgggctga gatcgatgcc
      301 ccggccaacc ttcccctgga gccgaacgac acgatcgtcg tgcccgaagc cgatcggcgt
      361 gtcaaggttg ctggttacgt gaaagacccc ggctacttcg agtaccgccc cggcgaaacc
      421 gtctcagccg ccctggcgat ggctgggggc gtcctcacca acatcggctc cgactcacgt
      481 gtccagctcc gctggccctc gggggcagaa gaggaagtcg atctgcgcca gagtgatcca
      541 cccctcaccc ccggtgtcga gatccgtgtg cccttcgcgc gcaatcgcgt cgtggtcctc
      601 ggctacgtcg agaaggccgg gttctatgaa tggcacgagg gcgacacggt catcgatgcc
      661 atcgccctgg ccggtggcat caaggggccg accacgaacg tgggtatgcg cctcgtcaag
      721 ggcgagtact atcgcgcgct cctgatacgg cgcgagaacg gcgtcgagaa acgctacgac
      781 ctggacctga agtcctacta cgagcgcggg gaccagacgc acaatctgag ggtctacccc
      841 gatgatgtca tcatggtgcc gggtaagggc aagttcaacc cgcaggccct gctgcaggac
      901 gtgttgctga tcccagactt cctgaatctg ttccggtgag cggccggggc gccgcgaaca
      961 ggaggccgat ggcgagtggt cgtgcggagc agggctcccg tgcgaatcga ctttgcgggc
     1021 gggtggactg acgtcgccgt gttcgccgac agtgtcggcg gggcggtggt cagcgcgacc
     1081 attgacaagt acgtgcacgg gcacatgcgg accatggatg gcggccagcg gccggcggac
     1141 ccggcgccgg acgccccggg ggcttgggag tggcaggtgc ctggcgaggg aatcaccgtg
     1201 gcctaccact cagacctgcc ggccggctca ggcttgggca cctcggccac gctcaacgta
     1261 gtgtggctct cactggtccg ggcccggcct gtcaacgaag ccgaccgcgc gaccctggcg
     1321 gaactggcct atgacctcga gcgaatcctc gggatcctgg gaggacggca agaccagtat
     1381 gccagcgcgt ttggtggggt gaactacctg acatttggcg gcaccaccgg tgtcg
//
LOCUS       DAOSPL010001402         1435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_90305_length_1435_cov_3.195652, whole genome shotgun sequence.
ACCESSION   DAOSPL010001402 DAOSPL010000000
VERSION     DAOSPL010001402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90305_length_1435_cov_3.195652"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="PLQ54_20145"
     CDS             complement(<1..1184)
                     /locus_tag="PLQ54_20145"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOS95632.1"
                     /translation="MVASLCALLCLGSASAPAGPTQFHVSPTGDDTNPGTRSRPFATL
                     ERARDAIRALKSEQGLPEGGITVVLHPGVHRRTAGFVLEEQDSGEPGKPVVYRSLDAG
                     AARLVGGLLIPPRAFAPVTDAAIITRLDASARAAVRQADLHALGVHELGAEWPDRFRG
                     YVGWPELFVDGVPMTLARWPNEGLARVARVIDSGSKPRWAESPDRPGVFEYEGDRPTR
                     WLKADRVYLNGYWAYKWYNECIRVDRIDPDARTITFAAPHLYGVGGPSGGEYYALNLL
                     EELDRPGEYFIDAQSGRLYLWPPEPLVRQEIGLSLLNEPLIQIRGASHITLRGLTLEL
                     SRDLAVTIEGGSDNRVAGCTMRNLATDAVRVSGGERNGVVACDIYDMGGGGVILNGGD
                     RAT"
     gene            complement(1229..>1435)
                     /locus_tag="PLQ54_20150"
     CDS             complement(1229..>1435)
                     /locus_tag="PLQ54_20150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOS95633.1"
                     /translation="PLGQRVDGFAIDARAAGEWHEVAAAQAIGPHRLVRFEPVEADAV
                     RLRITAAPVCPAIAEFAVFPEARG"
BASE COUNT          232 a          488 c          520 g          195 t
ORIGIN      
        1 gtggcccggt ccccgccgtt gaggatgacg ccaccgcctc ccatgtcgta gatgtcgcac
       61 gccacgacgc cgttgcgctc gccgccggac acccggacgg cgtctgtggc caggttacgc
      121 atcgtgcaac cagccacgcg gttgtccgag cccccctcga tcgtgaccgc caggtctcgc
      181 gagagctcga gcgtgaggcc gcggagcgtg atgtgagagg caccgcggat ctggatcagg
      241 ggctcgttga ggagcgacag gccgatctcc tgacgcacca ggggctccgg aggccacagg
      301 tagagcctcc ccgactgggc gtcgatgaag tactcgccag ggcggtcgag ctcttcgagc
      361 aggttgaggg cgtagtactc gcccccggac ggcccaccga ccccatagag gtggggcgcg
      421 gcgaatgtga tggtgcgcgc atcaggatcg atgcggtcga cgcgaatgca ctcgttgtac
      481 cacttgtagg cccagtagcc attgaggtac acgcggtcag ccttgagcca tcgggtcgga
      541 cggtcgccct cgtactcgaa gacgccgggc cggtcgggag actcggccca gcgaggcttc
      601 gagccggaat cgatcacgcg ggcgacacga gcgagaccct cgttgggcca acgggccagg
      661 gtcatgggta ccccatccac gaagagctcg ggccacccga catagccccg gaatcggtcg
      721 ggccattcgg cgcccagctc gtggacgccc agggcgtgaa ggtcggcctg gcgcacggcg
      781 gcgcgggccg aggcgtcgag ccgggtgatg atggcggcat ctgtgaccgg ggcgaaggcg
      841 cgtggcggga tgagcaagcc tccgaccaag cgcgccgcgc ccgcatcgag ggaccggtag
      901 accacgggct taccgggctc tcccgagtcc tgctcctcga ggacgaagcc agccgtgcgc
      961 ctgtggacac cggggtgcaa gaccacggtg atgccgccct ccgggagccc ctgctcgctc
     1021 ttgagggcgc ggatggcatc gcgagcccgc tccagcgtgg cgaagggccg gctccgggtg
     1081 ccggggttcg tgtcatcacc cgtgggcgac acgtggaact gcgtgggccc agccggtgcc
     1141 gatgcagagc ccaggcagag cagcgcgcac aacgatgcga ccatggttgg cctccggtac
     1201 ctgcaatcgg gcgaccggcc ggccgcccct aacctctcgc ctctgggaag acggcgaact
     1261 cggcgatcgc cgggcagacg ggcgcggccg tgatacgcag ccgaacggcg tcggcctcga
     1321 cgggctcaaa ccgaaccagc cggtgcggcc cgatcgcctg agccgccgcg acctcgtgcc
     1381 actcgccagc ggcgcgtgcg tcaatggcga agccgtcgac gcgctggccg agcgg
//
LOCUS       DAOSPL010001403         1430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_90741_length_1430_cov_3.488727, whole genome shotgun sequence.
ACCESSION   DAOSPL010001403 DAOSPL010000000
VERSION     DAOSPL010001403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90741_length_1430_cov_3.488727"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..>1430
                     /locus_tag="PLQ54_20155"
     CDS             138..>1430
                     /locus_tag="PLQ54_20155"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOS95634.1"
                     /translation="MVAVNRVAGPMILTLLMVAAAPAVGRADDLPWAIVDEHGVLRWE
                     DTRSEVCLWGVNYYAPFSISYANLNRTDTDPGQAVDQDLAHFSRMGLDAIRLHVFDRE
                     ISTADGNLILNDHLAVLDYLLLRARERRIRVVLTPIAWWYAPGTNGFSDHYTKDQLVR
                     DPEARRAQARYLRQFMLHRNPYTDLVYGQDPTIVAIEIINEPEYEPDTTDDEIVRYAN
                     EMAQAIRSTGAHQPIFYSDWNGRHEVIRRAKVDGATFGWYPTGLQSGRSLTRNYLPVL
                     GTHPTLAEAPEGKARIVYEFDAADVPGGYVYPAMARAFRSGGVQIATQFQYDPLGTAA
                     YNSDWDTHYLNLIYTPRKAMGFVIAGEVFRRTALFAPAQRQGLDAKAPDGAAGPFEDV
                     TIDAPTDTVVLNTPEVFMYSGTTDAEPRNPGRLERVAGY"
BASE COUNT          250 a          477 c          480 g          223 t
ORIGIN      
        1 ctaggcgggc gagcgggcgt ctggatggag aacgggaacc ggtagcggtc tcccatgccc
       61 cggcagactg aggccccttt cgcagatacc ggggcccggc tgaggccggg cgtctgatcg
      121 ggaggtctag cacaggcatg gtggcggtga accgcgtcgc agggccgatg atcctgacct
      181 tgctgatggt ggctgcggcg cccgctgttg ggcgggccga cgatctgccg tgggctatcg
      241 tggacgagca cggcgtgctg cgatgggagg acacccggag cgaggtgtgc ctgtggggtg
      301 tgaactacta cgccccgttc agcatcagct atgccaacct gaaccgcacc gataccgacc
      361 ccggccaagc ggtcgaccag gatctggccc acttcagccg catggggctc gacgccatcc
      421 gcctgcatgt cttcgaccgc gagatcagca cggccgatgg caacctgatt ttgaacgacc
      481 acctggccgt tctcgactat ctgctgctgc gggcccgtga gcggcgcatc cgcgtggtgc
      541 tgacacccat cgcctggtgg tatgccccgg ggacgaacgg cttctcggat cactacacca
      601 aggaccagct ggtgcgcgac cccgaggccc gcagagcgca ggcgaggtac ctcaggcagt
      661 tcatgctgca tcggaacccc tacacggacc tggtctacgg gcaagacccc accatcgtgg
      721 cgatcgagat catcaacgag cccgagtacg agcccgacac caccgatgac gagatcgtcc
      781 gctatgcgaa cgagatggcc caggccatcc ggtcgacggg tgcacaccag ccgatcttct
      841 acagcgactg gaacgggcgg cacgaagtca tccggcgggc gaaggttgat ggcgccacct
      901 ttggttggta tcccaccggg cttcagtcgg ggcggagcct gactcggaac tacctgcccg
      961 tgctgggtac acatcccacc ctcgcggagg cgcccgaagg caaagctcgc atcgtgtacg
     1021 agttcgacgc cgcggacgtg cccgggggct acgtgtaccc cgccatggcg cgagccttcc
     1081 gctcgggcgg cgtccagatc gccacgcagt tccagtatga tccgctgggc acggcggcct
     1141 acaacagcga ctgggacacg cactacctga acctcatcta cacgccccgc aaggcgatgg
     1201 gtttcgtcat cgccggcgag gtgttccgcc gcaccgcgct gttcgcgccg gcccagcgcc
     1261 aggggctcga cgccaaggcc cccgatggcg ccgccgggcc gtttgaggat gtcaccatcg
     1321 atgctccgac cgacaccgtg gtgctgaaca cgccggaggt gttcatgtac tcgggcacga
     1381 ccgacgcgga gccgagaaac ccggggcggc tggagcgggt ggcgggctac
//
LOCUS       DAOSPL010001404         1428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_90928_length_1428_cov_3.240350, whole genome shotgun sequence.
ACCESSION   DAOSPL010001404 DAOSPL010000000
VERSION     DAOSPL010001404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90928_length_1428_cov_3.240350"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="PLQ54_20160"
     CDS             <1..995
                     /locus_tag="PLQ54_20160"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOS95635.1"
                     /translation="RALLLAARELYDAPDINRDTVEGLSARIAASNRPGWYREVLSER
                     AGIAVSVQCGNTEGLDRELFNPVFHSDEWVMARTRFDFERLGQRTGVPIHTLDDLCAA
                     LSSALDAEQKAGICGMKSALAYVRILRYDKRTREEAERVFVRGFESHGGGVDWSEARP
                     LQDFLFHQVIREGIRRNLTIQIHTGLQEGNGNFITNANPTHLTGLFMEYPEARFDLFH
                     GGYPYGGELATLAKNFANVYVDMCWLHVISPTFAQRWLREWIETVPSNKISAFGGDYI
                     FVEGAYAHSRMARQGVAATLAGLVEDGYFGEDEAIAVARKILRDNPASLFGLKV"
     gene            1039..1236
                     /locus_tag="PLQ54_20165"
     CDS             1039..1236
                     /locus_tag="PLQ54_20165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95636.1"
                     /translation="MLRRLNTRAFTACLCLSLFGPPLPAVARPLPGPPPVIQDAKDRG
                     VFWEIVPRLRSPDRQTVFHAT"
     gene            1237..>1428
                     /locus_tag="PLQ54_20170"
     CDS             1237..>1428
                     /locus_tag="PLQ54_20170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95637.1"
                     /translation="MLVDIGDETCVGALAAGPGPALEAEREATVEQMEAASEILRLRL
                     AGGYLKHRALPAAATNRAGA"
BASE COUNT          228 a          496 c          483 g          221 t
ORIGIN      
        1 gccgcgcgct gctgctcgcc gcgcgagagc tctacgatgc ccccgacatc aaccgggaca
       61 ccgtcgaggg cctctccgcc aggattgcgg ccagcaaccg gcccggctgg taccgcgagg
      121 tgctgtccga gcgggcaggc atcgccgtgt cggtccagtg cgggaacacc gaggggctcg
      181 accgggagct cttcaacccg gtgttccact cggacgagtg ggtcatggcg cgcacgcggt
      241 tcgacttcga gcgcctcggg cagcgcacag gggtgcccat ccacacgctc gacgacctgt
      301 gcgccgcact ctcgtccgcc ctcgatgccg agcagaaggc gggcatctgc ggaatgaagt
      361 cggccctcgc ttacgtgcgc atccttcgct acgacaagcg cacgcgcgag gaggccgagc
      421 gggtcttcgt gagaggcttc gagtcacacg gcggcggtgt ggactggtcc gaagccaggc
      481 cgctgcagga cttcctgttc catcaggtga tccgcgaggg catccgacgg aacctgacca
      541 tccagatcca caccggtctg caggaaggca acgggaactt catcaccaac gccaacccga
      601 cacacctgac cggcctgttc atggagtacc ccgaggcccg cttcgacctg ttccacggcg
      661 gctaccccta cggcggcgag ctggccacgc tcgcgaagaa cttcgccaac gtctacgtcg
      721 acatgtgctg gctgcacgtc atctctccga ccttcgccca gcggtggctg cgcgagtgga
      781 tcgagacagt gccgtcgaac aagatctcgg ccttcggcgg ggactacatc ttcgtcgagg
      841 gcgcctacgc ccattcgcgc atggcccgcc agggcgtggc cgccacactg gccggcctgg
      901 tcgaagatgg ctacttcggc gaggacgagg ccatcgccgt ggcccgcaag atcctgcgcg
      961 acaacccggc gagcctgttt gggctgaagg tgtaggggcc ggcgtctagc cgggcgcttc
     1021 gccaggggag tgaggactgt gctgcgcaga ctgaacacgc gcgctttcac cgcatgcctc
     1081 tgtctctctc tgttcgggcc tcctctgccc gccgtggccc ggccgttgcc cggcccgccg
     1141 ccggtcatcc aggacgccaa ggaccgcggg gtgttctggg agatcgtgcc acggctcaga
     1201 tcgccggacc ggcaaaccgt cttccatgcc acgtaagtgc tcgtcgacat cggagacgag
     1261 acgtgcgtcg gcgcgttggc agccggcccg ggaccagccc tagaggccga gcgcgaggcc
     1321 actgtggagc agatggaggc ggcgtccgag atcctgcggc tgcggctggc cgggggctac
     1381 ctcaagcacc gggcactgcc ggccgcggcg acgaaccggg cgggcgct
//
LOCUS       DAOSPL010001405         1426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_91173_length_1426_cov_2.144420, whole genome shotgun sequence.
ACCESSION   DAOSPL010001405 DAOSPL010000000
VERSION     DAOSPL010001405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91173_length_1426_cov_2.144420"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..>1426)
                     /locus_tag="PLQ54_20175"
     CDS             complement(60..>1426)
                     /locus_tag="PLQ54_20175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95638.1"
                     /translation="VRRLLVTFVGRVAAGAEARYELRWSKDSPATPDEAPQPGVSVVE
                     LAGATVVRNGAGPGAMMLVVDGHRGCGLFESVAIDEAGDGFTDDLPLIGDRRPVTSSV
                     SYAGTRSHQAELGFSVPEVVVEESGPVRAVVRVRSEYDERVSMVHRLVVYAGLRAVFV
                     QTTLYATPGRPQGEAIRVFGHTNVLPLGMLESPDVTFGACSADAPAETRTSVVPGQPL
                     VIRSQQGSTGFSATLGGDPLADVAAPLGWVEAACRGRRVALCLGSLPARPAQRVDVGG
                     GYLVADSCASELSLAPGQSLTEEWLLYFPADLTALEPADAARALADPLCPIAVAKGSE
                     GLGGTRRPPDMTEPTLPTWGEAGYETRLERVLASAWHQADEVVAGRGAASSAEWVEAL
                     LWAFGQTGDPRWLGAARGLAIRLCDRPPPAGTGWRSAVAACAELTGDGRLLDALRPPD
                     RPEP"
BASE COUNT          238 a          550 c          450 g          188 t
ORIGIN      
        1 attggatgcg ctccatccct ggtctcggtg taggcctctg tctccatcat acaccgtcgc
       61 tatggttcgg gacggtccgg tgggcgcaac gcgtcgagga gccgtccgtc gcccgtcagt
      121 tcggcgcatg ccgctacagc gctccgccac cccgttcccg cgggcggagg tctgtcgcag
      181 agccgaatgg ccagcccccg ggccgccccg agccaccgcg ggtcgcccgt ctggccgaac
      241 gcccacagga gggcctcgac ccactcggcg ctcgaggctg cgccgcgtcc tgcgactacc
      301 tcgtccgcct ggtgccaggc cgatgccagg acccgctcca gccgcgtctc ataaccggcc
      361 tcgccccacg ttggcagcgt gggctccgtc atatcgggcg ggcgccgcgt tccgcccagc
      421 ccctcgctgc ccttcgccac ggcgatgggg cacagcggat ccgccagcgc ccgggctgca
      481 tctgccggct ccagtgccgt cagatccgcc gggaagtaca gcagccactc ctccgtgagt
      541 gactggcccg gcgcgaggct cagctccgac gcgcaagaat cggccaccag gtatccccca
      601 ccgacgtcga cacgctgggc tggccgcgcc ggcaggctgc ccagacataa ggcgacacgc
      661 cggccgcggc aggcggcttc gacccaccca agcggagcgg caacatcggc cagcggatcc
      721 cccccgagtg tcgcgctgaa accggtagag ccctgctgtg agcgtatgac aagcggctga
      781 ccgggcacca ctgaggtacg cgtctccgcc ggcgcatccg cgctgcacgc gccgaaggtc
      841 acgtcgggtg actccagcat tccaaggggc agcacattgg tgtgcccgaa cacgcgaatg
      901 gcctcgcctt gggggcggcc aggcgtcgcg tacagcgtag tctggacgaa gacggcccgt
      961 aagcccgcgt acaccaccag ccgatgcacc atcgacacgc gctcgtcata ctcggaccgg
     1021 acgcggacca ccgcgcgaac cggcccggac tcttcgacaa caacctccgg caccgagaag
     1081 cccagctcgg cttgatggga ccgggtgccg gcgtaggaca ccgacgaggt caccggccgg
     1141 cgatccccaa tcagcgggag gtcatctgtg aacccgtcgc cggcctcatc aatagctacg
     1201 ctctcgaaca gaccacaccc gcgatggcca tccaccacca gcatcatggc gcccggaccg
     1261 gctccgttgc gcaccacagt ggccccggcc aactcgacga cggagacacc gggctgcggc
     1321 gcctcgtccg gcgtcgcagg actgtccttc gaccaacgaa gctcgtagcg cgcctcggca
     1381 ccggccgcca cacggccgac aaaggtgacc aggagccggc gcacac
//
LOCUS       DAOSPL010001406         1425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_91220_length_1425_cov_3.500730, whole genome shotgun sequence.
ACCESSION   DAOSPL010001406 DAOSPL010000000
VERSION     DAOSPL010001406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91220_length_1425_cov_3.500730"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1425)
                     /locus_tag="PLQ54_20180"
     CDS             complement(<1..>1425)
                     /locus_tag="PLQ54_20180"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOS95639.1"
                     /translation="WLRGVRYIAMPTTVLAMGRCAALRQAGPYVVEVAVEPAEVDPDG
                     PAWFLDHRPIPAPVRLRTRCARQGEAVGPWQIETVDDPFIAGARGEAYWTTDVYADSS
                     ARNPFGHLLGTRTTPGARVLRPEVQLAAQAPERCALWRPWRTIRTGPERGGCWMALGD
                     VDGDGALDLVVARNDNQAVRALTAYTAHGTELWRWGEGGKADIAYDVPVTVYDIDGDG
                     ANEVLCGIEGFVLGLDGRTGREKFRRPLPEGLAVADCIIIANLRGRPRAEDLIIKSRY
                     DAVWAYTNVWQPLWSWRGNTGHHPAAGDVDGDGRDEVLCGFALLDDDGRELWTQPLPG
                     HADSVRIIPAGLGHGPRFLCTCCGGNDMALMDLSGRMLWHRQPERDPYHYQSTYVGRF
                     SRDIPGFEIVVDEGWARPGRSRIRVVSLEDGSLRGTCYTSYPRFAARADFDGAGLDEL
                     VFPSEGGIFDTRGRREVRLAHALP"
BASE COUNT          235 a          499 c          494 g          197 t
ORIGIN      
        1 ggggtagcgc atgggcgagg cgcacctcgc gacgcccacg agtatcgaag atgccgccct
       61 cggaggggaa gaccagctcg tcgaggccag cgccatcgaa gtcagccctg gcggcgaagc
      121 gggggtagct ggtgtagcag gtcccccgga gcgatccatc ctcgagagag accacgcgga
      181 tgcggctgcg gccgggacgc gcccagccct cgtcgaccac gatctcgaag ccagggatgt
      241 cgcgcgagaa acggcccacg taggtcgact ggtagtggta gggatcgcgc tcgggctgcc
      301 ggtgccagag cattcggccg gacagatcca tcagggccat gtcgttgccg ccgcagcagg
      361 tgcagaggaa cctcggtccg tggccaaggc cggcggggat gatgcgcacc gagtctgcgt
      421 ggcccggcaa gggctgagtc cacagttcgc ggccgtcatc gtcaagcagc gcgaagccgc
      481 acagcacctc gtcgcgtccg tcgccatcga catcgcccgc cgccggatgg tggccggtgt
      541 tcccgcgcca tgaccacagt ggctgccaaa cgttagtata ggcccacacc gcatcgtagc
      601 ggctcttgat gatgagatcc tcggcgcgcg ggcggccgcg cagattggcg atgatgatgc
      661 agtcggccac ggcgaggccc tcgggcagcg ggcggcggaa cttctcgcgg ccggtgcggc
      721 cgtcgagccc cagcacgaag ccctcgatgc cgcacagcac ctcgttggcg ccatcaccgt
      781 cgatgtcgta cacggtgacg ggcacatcgt aagcgatatc ggccttgcca ccctcgcccc
      841 atcgccacag ctcggtgccg tgggccgtgt acgccgtgag cgcgcggaca gcctggttgt
      901 cgttgcgggc gacgaccaga tcgagcgcgc catcgccatc gacgtcgccc aaggccatcc
      961 agcagccgcc gcgctcgggc cctgtgcgta tggtcctcca cggccgccac agcgcacacc
     1021 gctcgggcgc ctgtgcggcg agctgcacct cgggccgcaa gacccgcgca ccgggagtcg
     1081 ttcgtgtgcc caggagatgg ccgaaggggt tccgtgctga tgagtcagca tagacgtcgg
     1141 tagtccagta cgcttccccg cgggcaccag cgatgaaggg gtcgtcgacc gtctcgatct
     1201 gccacggccc caccgcctcc ccctggcgag cgcatcgcgt tcgcagccgc actggcgcgg
     1261 ggatgggccg gtggtcgaga aaccacgcgg ggccatcggg atcgacctcg gccggctcca
     1321 cagcgacctc gaccacataa gggccagcct gccgcagagc cgcgcaccgc cccatcgcca
     1381 gcacggtggt cggcatggcg atgtagcgca ccccgcgcag ccagg
//
LOCUS       DAOSPL010001407         1424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_91336_length_1424_cov_2.319942, whole genome shotgun sequence.
ACCESSION   DAOSPL010001407 DAOSPL010000000
VERSION     DAOSPL010001407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91336_length_1424_cov_2.319942"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..974)
                     /gene="amrB"
                     /locus_tag="PLQ54_20185"
     CDS             complement(105..974)
                     /gene="amrB"
                     /locus_tag="PLQ54_20185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOS95640.1"
                     /translation="MAIRPADRAGSWYPAGTTSCETRLRAMLDAAAAALPAPAGEPMA
                     GIAPHAGWDFSGPTAALTFGFLQASDAQVVALFGADHYGLRNAALMSSGTWQTPVGEV
                     RVDVGLGRAILEEAGRIVADDPEAHFDEHSIEILMPFVRHCFPEATVVPIIVPRGAPA
                     TEIGDAVGRVLAGSKRRAVAVGSTDLTHYGPNYGFAPRGTGAESLEWVRNENDRRFLD
                     LAEAMRATELVSYAEASRAACGARASAAAIACGQALGATRGMVLGHTTSHDVLPLGRP
                     TSFVGYGAVGYLR"
     gene            complement(998..>1424)
                     /locus_tag="PLQ54_20190"
     CDS             complement(998..>1424)
                     /locus_tag="PLQ54_20190"
                     /inference="COORDINATES: protein motif:HMM:NF018404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HOS95641.1"
                     /translation="IAGGNTPAQLVLTAGGIIPADNLAPNIVLTVSLSVVLAALLVHK
                     FRPRLVHRCSFCGRLDSEVEEMVSSGGVSICGECVVRTVQRMRRTQRPAEVASPAPSA
                     LADEAEEARRRERADRVAFRDPQELIDLSVDSSDEDSTA"
BASE COUNT          253 a          520 c          449 g          202 t
ORIGIN      
        1 gccacgtgca ttggggggag gtgggtgcac tcagagatcg gggacattgc cgtttccagc
       61 gaagacccca aggcgatgcc cttaccgcga tcggaacgcc agacctaccg cagatagccg
      121 accgcgccgt agcccacgaa actcgtcggg cgccccagcg gcaacacgtc atggctcgtc
      181 gtgtgtccaa ggaccatgcc tcgagtggct ccaagtgcct ggccgcacgc gatggccgcg
      241 gcagaggccc gggcgccaca tgccgcgcgt gaggcctccg catacgagac cagctcggtg
      301 gcccgcatcg cctctgccag gtcgaggaaa cgccgatcgt tctcattgcg gacccactcg
      361 agcgactccg caccggtgcc tcgcggcgcg aatccatagt tggggccgta gtgcgtgaga
      421 tccgtagaac ccaccgcaac cgcccggcgc ttcgatcccg cgaggaccct gcccaccgcg
      481 tcaccaatct cggttgccgg cgcgccccgc ggcacgatga tgggcaccac cgtcgcctcg
      541 gggaagcagt ggcggacgaa gggcatcagg atctcgatgg agtgctcgtc gaagtgggcc
      601 tcggggtcat cggccacgat ccgacccgcc tcctccagaa tggctcggcc cagccccaca
      661 tcgacacgga cctcgcccac cggcgtctgc cacgttcctg aactcatgag cgctgcattc
      721 ctcagcccat agtgatcggc gccaaataac gcaacaacct gggcatccga tgcctggagg
      781 aaaccaaacg tgagtgccgc agttggcccc gagaagtccc agccggcatg cggtgcgatg
      841 cccgccatgg gttcgcccgc cggcgcggga agcgccgccg ccgcggcatc gagcatcgcc
      901 cgcaggcgag tctcgcacga cgtagtgccc gcggggtacc agctccccgc gcggtcagcc
      961 ggtcggatcg ccatgggaac ctggcctccc aatgccctca ggcagtggag tcctcgtcgg
     1021 aggagtcgac gctgaggtcg atgagctcct gcgggtcgcg gaacgccacc cggtcggcac
     1081 gctcgcgccg gcgcgcctcc tctgcctcgt ccgccaacgc ggaaggcgcc ggagacgcca
     1141 cctccgccgg ccgctgggtc cggcgcatgc gctgaaccgt ccgcacgaca cattcgccgc
     1201 agatcgagac tccacccgac gagaccatct cctcgacctc gctatcgagc cggccgcaga
     1261 acgaacaacg atggaccagc ctcggccgga acttgtgcac caacagggcg gccaacacaa
     1321 cgctcaagct caccgtgagc acgatgttcg gtgccaggtt gtccgcgggt atgatgccgc
     1381 ccgccgtcag aaccaactgg gccggagtgt tccctcctgc gatg
//
LOCUS       DAOSPL010001408         1421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_91583_length_1421_cov_4.822108, whole genome shotgun sequence.
ACCESSION   DAOSPL010001408 DAOSPL010000000
VERSION     DAOSPL010001408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91583_length_1421_cov_4.822108"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLQ54_20195"
     CDS             <1..540
                     /locus_tag="PLQ54_20195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95642.1"
                     /translation="LRAESNPSAVCTEVQGVSVCLHRAKAALLSPLADDVNAILSTVD
                     GKPAVPLTHIVDASLRPAPEPGLVSLELQTEQSDWQDWAAGDVAGYLTGRPACGPQGD
                     LTGGDVDTGQGRTAVSLAFATWIARTAGFNPPHLAADQGVAPTPNNLDARSEREVAAL
                     YHEHSTELATCTLTPDALG"
     gene            complement(588..851)
                     /locus_tag="PLQ54_20200"
     CDS             complement(588..851)
                     /locus_tag="PLQ54_20200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95643.1"
                     /translation="MGDPGDGARSEKGDCSEQPCPPSEAVMRECFPAGEPGRDERRGD
                     DCEQNGDGFPDGGEARNLGVVETDFEDEDVGLKYQRDADEIQR"
BASE COUNT          257 a          552 c          380 g          232 t
ORIGIN      
        1 ctaagagccg aatccaaccc atccgctgtg tgcaccgagg tccaaggagt ctcggtatgc
       61 cttcaccgcg ccaaagccgc gctcctgagt cccctagccg acgacgtcaa cgccattctc
      121 agcacagtgg acggcaagcc ggccgtcccc ctcacccaca tcgtcgatgc ctccctgcgg
      181 ccggcccccg aacccggcct ggtctcgctc gaactgcaaa cggagcaatc agactggcag
      241 gactgggctg ccggtgacgt cgccggctac ctcacggggc gcccggcctg cggccctcaa
      301 ggagacctca ccggcggcga cgttgacacg gggcaaggcc gcaccgccgt cagcttggcc
      361 ttcgcaacgt ggattgcacg caccgcaggg ttcaacccac cacatctggc agccgaccaa
      421 ggcgtcgcac ccacacccaa caaccttgat gcgcgctccg agcgagaagt cgccgcgctg
      481 tatcacgaac actccacgga gctcgccacg tgcacactca cccccgacgc cttgggctga
      541 tgccgggatg gcgcctgtat ctctcctctc gaacgcttcc aactctctta gcgctgtatc
      601 tcgtcagcgt cgcgctggta cttcaagccc acgtcctcgt cttcgaaatc agtctcgaca
      661 acaccaagat tgcgcgcttc acccccgtct gggaagccgt ccccgttctg ctcgcagtca
      721 tcgccccggc gctcatcgcg ccccggctcg ccagctggga agcactcgcg catcaccgcc
      781 tcgcttggcg ggcagggctg ctcggagcag tcacccttct cgctccgagc accatcccct
      841 ggatcgccca cctcaacctt ccccccgatg cccggtggtg ggacatctcc tggaacgtgc
      901 tcttcctagg ctcccttgcg ctcaccgcaa cagccgtgct gggccgcctc ggtggaccag
      961 ccacgggcct agcgatctac gccaccctca tcgcggtaca gcagaccgct cccaacgccg
     1021 cagcatccct ccccttcagc ggagcgagca ccaacctgca accccacccc ggccccacat
     1081 ccgccgcggc ggtcatcgcc atagccgtat ggaccgtcac cctcggccga tcgcgccgac
     1141 tcagacttcg agagcgaaac gactgagtca aagccactac aggatgctct ctggctttca
     1201 gaggtggggc cagatcaagc cgtcatgccg gggccagttc tacttgacac tcacaaggca
     1261 cagagcacac cggcattgtc caccagccga caaccgccct tccgctattc ggcatgaccg
     1321 gaagggtctg cggcccttcc ggcctattgc gaaccgccgc ccaaaggggt cacgtcgtgg
     1381 gtggatcaca tggccgcagg ggtctggaca taggccctca g
//
LOCUS       DAOSPL010001409         1416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_92136_length_1416_cov_3.104335, whole genome shotgun sequence.
ACCESSION   DAOSPL010001409 DAOSPL010000000
VERSION     DAOSPL010001409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92136_length_1416_cov_3.104335"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PLQ54_20205"
     CDS             <1..671
                     /locus_tag="PLQ54_20205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95644.1"
                     /translation="VGDVVVEVAGHRSNKVPISEWTIPFTYTRTWYGSLTDTIHFTIH
                     LRADVHKFREQPGKTPVTPSAVLVKSMADSDGDWTSGGSGHLDNDGDAASITWSGSGV
                     LTGTDPWATAGSVGAWGSIDVQSMALYLWFWTLADQKQVDTVYPGGSTSSNTEVVSTN
                     SLPVYDETDNGPHLRIPLTSDFGIDAGSRNAPNPPEFQSATLEWEAAPVSYPPVESVH
                     ASGR"
     gene            complement(709..1395)
                     /locus_tag="PLQ54_20210"
     CDS             complement(709..1395)
                     /locus_tag="PLQ54_20210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOS95645.1"
                     /translation="MLCFGDSNTWGYDPASGQRLPAEQRWTGVLQAELGHDYCVIAEG
                     QNGRTTVWDDPIEGTRFAPGGHGKNGARYLLPCLESHHPVDLVVLALGVNDLKARFSL
                     PAEDIARACGQLIDMVRACPFGPGGGAPEVLLLAPAPVGKLTAFATMFAGAQEKSRLM
                     GRYYRAQAEERGCGFLDAGEIITTSDLDGIHFEAPEHAKLGKAVAEAVRLMMEQDSGN
                     SGRPPFPSEP"
BASE COUNT          237 a          442 c          513 g          224 t
ORIGIN      
        1 cggtcggcga cgtggtcgtc gaggtcgccg ggcacaggag caacaaggtg ccgatatccg
       61 aatggacgat cccgttcacc tacacgcgca cgtggtacgg gtcgttgacc gacaccattc
      121 acttcacgat ccacctgagg gccgacgtcc acaagttccg cgagcaaccg ggcaagacgc
      181 cggtgactcc gtcggccgtt ctggtcaagt ccatggccga tagtgacggt gactggacgt
      241 cgggaggatc cgggcacctc gataacgacg gcgatgctgc cagtatcacg tggagcgggt
      301 cgggcgtcct caccggcacc gatccgtggg cgaccgcggg gagtgtcggt gcgtggggct
      361 cgatcgacgt gcagtcgatg gcgctgtacc tgtggttctg gacgctcgcc gaccagaagc
      421 aggtcgacac ggtctatccg ggtggcagca cttcgtcgaa cacggaggtg gtgtcgacga
      481 actcgctgcc cgtgtacgac gagacggaca acggccccca tctccggatt cccctgacca
      541 gcgacttcgg catcgacgcg ggttcgcgca acgcgccgaa cccgccggag ttccagtcgg
      601 cgacactgga gtgggaggcg gcgccggtga gctacccacc ggtggagagc gtgcacgcca
      661 gcgggcggta gacggacggc ggatggcgga tggcagtgag gaccgggatc agggctccga
      721 ggggaatggc gggcggcctg agttgcccga gtcctgctcc atcatgagcc ggacggcttc
      781 ggcgacggcc ttgccgagct tggcgtgctc tggggcctcg aagtgaatgc cgtcgaggtc
      841 gctggtggtg atgatctcgc cggcatccag gaagccgcag ccgcgctctt cggcttgggc
      901 gcggtagtag cggcccatga gccgtgactt ctcctgcgca ccggcgaaca tggtggcgaa
      961 ggcggtcagc ttcccgaccg gggccggggc gagtaggagc acttccggcg cgccaccgcc
     1021 ggggccaaag gggcaggcgc ggaccatgtc gatgagctgg ccgcaggcgc gggcgatgtc
     1081 ttcggccggc aaggagaagc gagccttgag gtcattcaca cccagggcga gcacgaccag
     1141 gtcaaccggg tggtggctct cgagacacgg tagcaggtag cgggcgccat tcttgccgtg
     1201 accgcctggc gcgaaccgcg tgccctcgat ggggtcatcc cacaccgtgg tgcggccgtt
     1261 ctggccctcg gcgatgacgc agtaatcgtg gccgagttcg gcctggagca caccggtcca
     1321 gcgttgctcg gcgggaagtc tctgccccga cgcggggtcg taaccccagg tgttggagtc
     1381 gccgaaacac agcacggtct tcctggcaac accctc
//
LOCUS       DAOSPL010001410         1416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_92149_length_1416_cov_2.764144, whole genome shotgun sequence.
ACCESSION   DAOSPL010001410 DAOSPL010000000
VERSION     DAOSPL010001410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92149_length_1416_cov_2.764144"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PLQ54_20215"
     CDS             complement(<1..519)
                     /locus_tag="PLQ54_20215"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOS95646.1"
                     /translation="MTRWALLLALCGQARLWAAPAPPDPARFEAVIQAFEERDREQAP
                     PQGAILFYGSSSIVGWDTAASFPDLVTINRGFGGSHMSDAVHFANRIAIPYKPRLIVV
                     YEGDNDLAYGTPPETVRDGFLELVAKVRAALPDTRFAYLSTKPSAARWNIVEKIRQAN
                     ELIREAIARDPLC"
     gene            complement(516..>1416)
                     /locus_tag="PLQ54_20220"
     CDS             complement(516..>1416)
                     /locus_tag="PLQ54_20220"
                     /inference="COORDINATES: protein motif:HMM:NF036726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95647.1"
                     /translation="RQAHAAMGVPDDVTRETCLQAQSFSLNYRDAHRGRWGVFRNQLY
                     WLRHYPACRLFRLGRFEFMLQPFSGAVEVYRRRDTGQALALSEDGVRYDAQGYRPIND
                     AEPEGGFTGQLRHGDGLVEGTPISPLGMAIDRTVRLDLTHWEPVLKRGDTVLDMHIPA
                     GGNMTPERAKDSFERAVRFFAEQFPDRPAHAIACGSWIYNTQFEELLGPEANLVKHMR
                     EVYLFPIASSGRDGLWFLFYTDDVDPATARRDSSVRRAVLDHLAAGKHLRAGGMFMLT
                     CDVCHYGTQWYRQNSSFEGGWDA"
BASE COUNT          234 a          440 c          512 g          230 t
ORIGIN      
        1 gcagagcgga tcccttgcga tggcctcgcg gatcagctcg ttcgcctggc ggatcttctc
       61 gacaatgttc caccgggccg cgctgggctt cgtcgacagg taggcgaagc gggtgtcggg
      121 cagcgcggct ctgaccttgg cgaccagttc gaggaagccg tctcgcacgg tctcgggcgg
      181 cgtgccgtaa gccaggtcgt tgtctccctc gtagacgacg atgagccggg gcttgtatgg
      241 gatggcgatg cgattggcaa agtggaccgc atccgacatg tggctgccgc caaacccacg
      301 gttgatggtg accagatcgg ggaagctcgc tgccgtgtcc cacccgacga tactggagct
      361 gccgtagaag aggatcgcgc cctggggcgg ggcttgctcg cgatctcgct cttcgaaggc
      421 ctggatgacc gcctcgaacc gggcgggatc cggcggggcg ggggcggccc agaggcgggc
      481 ttgcccgcat agggccagaa gcagggccca tcgtgtcacg cgtcccaccc cccttcgaac
      541 gagctgttct ggcggtacca ctgggtgcca tagtggcaca catcgcaggt gagcatgaac
      601 atgccccccg cacggaggtg tttgccggcg gccaggtgat cgaggacggc ccggcggaca
      661 ctcgagtcgc gccgagcagt cgcggggtcc acatcgtccg tgtagaagag gaaccagagg
      721 ccatcccgcc cgctggaagc gatcgggaac aggtagacct cgcgcatgtg tttgacgagg
      781 ttcgcctcgg ggccgaggag ctcttcgaac tgcgtgttgt agatccaaga gccacacgcg
      841 atggcgtggg cggggcgatc ggggaactgc tccgcgaaga accgcacggc acgctcgaag
      901 gagtccttgg ctcgttcggg ggtcatgttc ccgcccgccg ggatgtgcat atcgagcacg
      961 gtatcccccc gcttcagtac cggctcccag tgggtcagat cgagccggac ggtgcgatcg
     1021 atggccatgc cgagcgggct gattggagtg ccctcgacga ggccgtcgcc gtgccggagc
     1081 tggccggtga agccgccctc gggctcggcg tcgttgatgg gccggtagcc ctgagcatcg
     1141 taacggacgc cgtcttcgga cagcgcaagc gcctgccccg tgtcgcgacg ccgatagacc
     1201 tcgacggcgc cgctgaacgg ctgcagcatg aactcgaagc gtcccagccg gaacagccgg
     1261 caggcagggt agtggcgcag ccagtagagc tggttccgga agacgcccca tcggccccgg
     1321 tgagcatcgc ggtagttgag cgagaacgac tgggcctgca gacacgtctc gcgagtgacg
     1381 tcgtcgggca cgcccatggc ggcgtgggct tggcgg
//
LOCUS       DAOSPL010001411         1415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_92205_length_1415_cov_4.003676, whole genome shotgun sequence.
ACCESSION   DAOSPL010001411 DAOSPL010000000
VERSION     DAOSPL010001411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92205_length_1415_cov_4.003676"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PLQ54_20225"
     CDS             complement(<1..490)
                     /locus_tag="PLQ54_20225"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOS95648.1"
                     /translation="MMPFRFGCQTYTWEMLGHRWKGTPDEILDTVAETGYQGVEFSNA
                     MIGCYGRHPAAFREALAARGLDCAAFAYARTGFTDPTRWEEDLEGAVEALRFCVAVDA
                     LLCLGGPAAPSRDHYETDFAQAVRFYNEVALRGADLGVTVCVHPHSHHGSLVESAEEY
                     ARL"
     gene            complement(515..>1415)
                     /locus_tag="PLQ54_20230"
     CDS             complement(515..>1415)
                     /locus_tag="PLQ54_20230"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS95649.1"
                     /translation="RAERVARAYGAKPFSNCAAMLDALNPDAVCACGMPDMHHGVAMQ
                     VLERGIPVWIEKPPAPTLHQAKELAAAAHRAGTFGMVGFMKRFAPANVVTKEYLDSGR
                     FGALASVLLIHGSGPYGHLRPMLMFNGIHPIDSVRYFGGEVEAVHAFAHASTDGVQSV
                     VVNLRFASGVPGQLNLNSGGTWSDCYEYTYLAGHQAQATIVGCREAEIMSPAGRFAQV
                     PGIMPFGWSNRYYVSGTMSHWEAGGHYTRGYFGELDHFVRAVRGEVQPVATLADGVEA
                     MRLIDAIIASVEGRREVLLSDIV"
BASE COUNT          243 a          476 c          481 g          215 t
ORIGIN      
        1 gcagccttgc gtactcctcg gcggactcga ccagggaccc atggtgcgag tgcgggtgga
       61 cgcagacggt cacacccagg tcggcgccgc ggagtgcgac ctcgttgtag aaccgcacgg
      121 cctgggcgaa gtcggtctcg tagtggtcac gcgagggcgc ggccgggcct cccagacaca
      181 gcagcgcgtc gacggcgaca cagaagcgga gcgcctcgac ggcgccctca agatcttctt
      241 cccaccgcgt ggggtcggtg aaccctgtgc gggcgtacgc gaaggcggcg cagtccaggc
      301 cacgtgccgc cagtgcctcg cggaacgccg cggggtgccg gccgtagcac ccgatcatcg
      361 cgttcgagaa ctccacgccc tggtagcccg tctcagccac ggtgtcgagg atttcgtcgg
      421 gggttccctt ccaccgatgg ccgagcatct cccaggtgta tgtctggcat ccgaaacgaa
      481 acggcatcat ggcctccctc gcgaaccgcg cgccctacac aatgtcgctc aggagcacct
      541 cgcgccgacc ctcgacgctg gcgatgatgg catcgatgag gcgcatggcc tcgacgccat
      601 cggccagagt ggccacaggc tggacctcgc cacgcacggc tctcacgaag tgatccagct
      661 cgccaaagta cccgcgcgtg tagtggccac cggcctccca gtgggacatg gtgccggaca
      721 cgtagtagcg attgctccag ccaaagggca tgataccggg tacctgcgcg aaccgcccgg
      781 caggggacat gatctcggcc tcacggcacc cgacgatggt cgcctgggct tggtgcccag
      841 ccaggtaggt gtactcgtag cagtcagacc aggtaccgcc gctgttgagg ttgagctgac
      901 ccgggacacc cgaggcgaac cgcaggttca cgacaacgga ctgcacaccg tcggtgctgg
      961 cgtgggcgaa tgcgtggacg gcctcgacct cgccgccgaa gtatcgcacg gagtcgatgg
     1021 gatggatccc gttgaacatc agcatgggcc ggagatgccc ataggggccc gagccgtgga
     1081 tgagcaggac cgacgccaat gcgccgaagc gcccgctatc gaggtactcc ttcgtcacga
     1141 cgttggctgg cgcgaagcgt ttcatgaacc cgaccatgcc gaatgtcccg gcgcggtggg
     1201 cggcggcggc cagctccttg gcctggtgca gcgtcggagc cggcggcttc tcgatccaga
     1261 ccgggattcc gcgctcgagc acctgcatgg caacgccgtg gtgcatatcg ggcatgccgc
     1321 aggcacacac ggcgtcgggg ttcagcgcgt ccagcatcgc cgcgcagttc gagaagggct
     1381 tggccccgta ggcccgggcc acgcgttcgg cccgg
//
LOCUS       DAOSPL010001412         1413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_92421_length_1413_cov_2.551546, whole genome shotgun sequence.
ACCESSION   DAOSPL010001412 DAOSPL010000000
VERSION     DAOSPL010001412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92421_length_1413_cov_2.551546"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1413)
                     /locus_tag="PLQ54_20235"
     CDS             complement(<1..>1413)
                     /locus_tag="PLQ54_20235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95650.1"
                     /translation="SVECGVLLDADGDPLPITVQISKNFAGEREEPFYNPTDTPFSET
                     FFPLHLAAGETREVTSLHLYQNWGNHPLKQLSSLGAWMDYYHSSTGVTETTCFVPFKF
                     GGLPGVAIADLRAMSQPMWASQPQHDNVAGHRFLMVRSGGDWHYAEYRGTEFRSTGPN
                     WMDISLDYLLDDGRIRARLDSFELPQTDELRNFLRLRCEVLAPISIADLATDFRLMDI
                     TSAIQGLRYKWVAWQSGPGQVETRELHAADEVAVPGVALGGDFPWATVYGDKRGCNSF
                     VVRRFTAQLGGEERGPAMVCHTTRQGDSQLALTVAGGAAELEPGDFVEAEFTLMPYGT
                     DQNQYETPERDRVPFGENAPRVLSVATGHKLADFPARVRAEGGAAEFTIGGGVNTIPV
                     IVEGLPTYRHPRMEERVAERYIPVAQSSVGEDGFQVFVDDEGTFGCVFLVNADGREHT
                     YRVAQAEPSAIEVSPLPTAPG"
BASE COUNT          220 a          470 c          494 g          229 t
ORIGIN      
        1 ccgggcgcgg tgggcagggg gctcacctcg atggccgaag gctcagcctg cgcgacccgg
       61 tacgtgtgct cccggccatc ggcgttgacc aaaaagacgc agccgaaggt gccctcgtcg
      121 tccacgaaca cctggaagcc gtcctctccc accgaggact gggccacggg gatgtaccgc
      181 tcagcgaccc gctcctccat tcgggggtgc cggtaggtcg ggaggccctc gacgatgacc
      241 gggatggtgt tcacgccgcc gccgatcgta aactccgctg cgccgccctc ggcccggact
      301 cgagcaggga agtccgccag cttgtggccg gtggcgaccg atagcacacg cggcgcgttc
      361 tcgccgaagg gaacccgatc gcgctcgggg gtctcatact ggttctggtc ggtgccgtag
      421 ggcatgaggg tgaactcggc ctcgacgaag tcgccgggct caagctcggc cgctccgccc
      481 gccaccgtga gggcaagctg cgagtccccc tggcgggtcg tgtggcacac catcgccgga
      541 ccacgctcct cgcccccgag ctgcgcagtg aaacggcgaa ccacgaagct gttgcagccg
      601 cgcttgtcgc catagaccgt ggcccaaggg aagtcgccgc ccagggccac gccgggcacc
      661 gcaacttcgt cggcggcatg cagctcgcgg gtctctacct gccccgggcc cgactgccag
      721 gcgacccact tgtaccgcag gccctggatg gcgctggtga tgtccatgag ccggaagtcg
      781 gtggccaggt cggcgatgct gatgggggcc agcacctcgc agcgcaggcg caggaagttg
      841 cgcagctcgt ccgtctgcgg cagctcgaag ctgtccagcc gggcgcggat gcggccgtcg
      901 tcgagcaggt agtccaggct gatatccatc cagttcgggc cggtgctgcg gaactcggtc
      961 ccccgatact cggcgtagtg ccagtcgccc ccgctgcgaa ccatcaggaa ccggtgtccg
     1021 gcgacgttgt cgtgctgcgg ctgggaggcc cacatgggct ggctcatggc ccgcaggtcg
     1081 gcgatggcga cgcccggtag gccgccgaac ttgaagggta cgaagcatgt ggtctcggtc
     1141 acaccggtgc tgctgtggta gtagtccatc cacgcgccga gagacgagag ctgcttcagc
     1201 gggtggttcc cccagttctg gtagaggtgc agtgaggtga cctcccgggt ctctccggca
     1261 gcgaggtgca gcgggaagaa ggtctcgctg aacggggtgt cggtcgggtt atagaacggc
     1321 tcctccctct cgccggcgaa gttcttgctg atctgtacgg tgatgggcag cggatcgccg
     1381 tccgcgtcca gcagcacgcc gcactcaact gaa
//
LOCUS       DAOSPL010001413         1412 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_92505_length_1412_cov_2.935151, whole genome shotgun sequence.
ACCESSION   DAOSPL010001413 DAOSPL010000000
VERSION     DAOSPL010001413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92505_length_1412_cov_2.935151"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="PLQ54_20240"
     CDS             complement(<1..1023)
                     /locus_tag="PLQ54_20240"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOS95651.1"
                     /translation="MATDPRLITTGRPIPSEGYADQPYVVQTDDGAWLCVMTTGPGVE
                     GAAGQHIIALRSTDRGQTWSRPVDIEPSDGPEASYAVLLKVPFGRVYCFYNHNTDRVA
                     EVRREDGGIYTRVDSLGHYVFKYSDDHGRTWSAERYEVPVREFACDRHNIYGGALRFF
                     WNVGRPLVLGDAAIMVLHKVGAMGAGFFSQSEGVFLRSRNILTERDPAAIEFETLPDG
                     DVGLRAPAGGGRVAEEQSVTALSDGSLFCVYRTADGWPACAYSRDGGHTWTPPAYHTY
                     SPGGRRVKHPRAANFVWRCANGRFLYWFHNHGGGPVAQMPEWGPYDDRNPAWLMAGHE
                     VETPGGL"
     gene            1163..>1412
                     /locus_tag="PLQ54_20245"
     CDS             1163..>1412
                     /locus_tag="PLQ54_20245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95652.1"
                     /translation="MRRVPGTLVLGMAAMGCGMGLAAPARYEITVTARDYAVGQAPVL
                     AELGRPAMMPRGASLRAPDGTRVPCQLVTDGEATQLVFV"
BASE COUNT          212 a          456 c          511 g          233 t
ORIGIN      
        1 cagaccaccg ggggtctcga cctcatgtcc cgccatgagc caggccggat tgcggtcgtc
       61 ataggggccc cactcgggca tttgggccac aggcccgccg ccgtggttgt ggaaccagta
      121 gaggaatcgg ccgttggcgc atcgccagac gaagttggcc gcccgcgggt gtttgactcg
      181 ccgcccgccg ggtgagtagg tgtggtacgc cggtggcgtc cacgtgtggc cgccatcgcg
      241 gctgtaggcg caagcgggcc agccatcggc ggtacgatag acgcagaaga gggaaccgtc
      301 gctgagggcg gtcacgctct gttcctcggc gacgcgcccg ccaccggcgg gcgcccggag
      361 gccgacgtcg ccgtcgggga gggtctcgaa ctcaatggcg gcgggatcgc gctcggtcag
      421 gatgttgcgg cttcggagga acacgccttc ggattgggag aagaagcccg cgcccatggc
      481 acccaccttg tgaaggacca tgatcgcggc atcgccgagc accagagggc ggccgacgtt
      541 ccagaagaac cgcaatgccc ccccgtagat gttgtggcgg tcgcaagcga actcgcgcac
      601 cgggacctcg tacctctcgg ccgaccacgt acgaccatgg tcgtcagagt acttgaacac
      661 atagtggccg agcgagtcga cgcgcgtgta gatgccgccg tcctcacgtc tcacctcggc
      721 gactcggtcc gtgttgtggt tgtagaaaca gtagacgcgg ccaaagggga ccttgagcag
      781 gaccgcatag gacgcctcgg gaccgtcgga gggctcgata tcgacgggtc tggaccaggt
      841 ctggccccgg tcggtgctgc ggagcgcaat gatgtgctga ccggcggcgc cctcgactcc
      901 ggggccagtc gtcatgacac acaaccacgc gccatcgtcc gtctgcacga cgtagggctg
      961 gtcggcatag ccctcactcg ggataggtct acccgtggtg atgagccggg gatctgttgc
     1021 catggcggtt cctccctttg cgccgccgcg cgcgggacgc gggtttccct tcgccgaggg
     1081 actgcccgcg cccctcgcga atgcacttcc ggtgtggccg cggcgccgcg gccggactgt
     1141 tcacgagggg tgcgtggttt gcatgcgtcg tgtccctgga acgctggttc ttggcatggc
     1201 cgccatggga tgcgggatgg gcctggcggc gccggcccgt tacgagatca cggtgacggc
     1261 ccgggactat gcggttggcc aggcaccggt cttggccgag ctgggccggc cggcgatgat
     1321 gccgcgcggt gcctcgctga gggcgcccga cggcacgcgg gtcccctgcc agctcgtgac
     1381 cgacggcgag gcgacgcagc tcgtgttcgt cg
//
LOCUS       DAOSPL010001414         1407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_92964_length_1407_cov_3.061391, whole genome shotgun sequence.
ACCESSION   DAOSPL010001414 DAOSPL010000000
VERSION     DAOSPL010001414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92964_length_1407_cov_3.061391"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLQ54_20250"
     CDS             <1..447
                     /locus_tag="PLQ54_20250"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOS95653.1"
                     /translation="NGFYAPTLADLVEVWFDGRPPMTQGRAPHSPAVFAALLAPPDRR
                     KGSPTSWRTLDAPPAGAGTTAASAAYMEGGYSVLAHIPLEVLGCQARGDLELKGIEIA
                     LDDADSGTRETQAMWAGGPDAFVDASMFGAMTLRPERVGWYRAILR"
     gene            470..>1407
                     /locus_tag="PLQ54_20255"
     CDS             470..>1407
                     /locus_tag="PLQ54_20255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95654.1"
                     /translation="MDLTRRGFLAAAGLAAVAPMAADGVAGLEGDPTPPEVPPFTGEP
                     YVLAGRRLVFTNWHFIRPGSFGWYDAAGNNVTVGGSEGPMGAQLRTHDMPVGIRLMAK
                     PAQRRGPLIEPGKPWETVGISLSTIIRDGAVFRAWGTCQGEKGRFQCVFESDDGLDWR
                     RPEAGVVEVDGSTANNLIGPPWWGGTVFIDPSAPENERYKAVTEGRISANDFEAYRER
                     RPGAWERRAQRADVGQIFAVLGSISPDGLHWTNQPEPLVVEHSDTQIVAHYDTRLRQY
                     VIYTRNWSVGERDPSAPDDRRLSWLSVGRRSIGRTAA"
BASE COUNT          208 a          503 c          463 g          233 t
ORIGIN      
        1 aatggcttct acgcgcccac gttggcggat ctggttgagg tatggttcga tggccggccg
       61 ccgatgactc agggccgcgc cccccattcc cccgccgtct tcgcggcgtt gctggcccct
      121 cctgatcgcc ggaaagggtc tccaacctct tggcgcaccc tcgatgctcc tccggccggc
      181 gccggcacga ccgcggcctc ggccgcgtac atggagggtg gctacagcgt tctggcacac
      241 atacctctcg aagtgttggg gtgccaggct cgcggagacc tcgagctcaa gggcatcgag
      301 atcgcgctcg atgatgccga tagcgggacc cgcgagactc aggccatgtg ggccggtgga
      361 cccgatgcct ttgtggatgc ctcgatgttc ggagccatga ccctgcgccc cgagcgagtg
      421 ggttggtacc gcgcgatcct gcgctgagcg ccgactggag ggatctcaga tggatctcac
      481 gcgacgaggg ttcctcgccg cggcgggtct cgccgccgtg gctccgatgg ctgcagacgg
      541 cgtcgccggt ctcgagggtg accccacacc tcccgaggtc ccgcccttca ccggcgagcc
      601 gtatgtcctg gcagggcgcc gcctcgtctt caccaactgg cacttcatcc gcccgggcag
      661 cttcggctgg tatgacgccg ccggcaacaa cgtgaccgtc ggcggctccg agggccccat
      721 gggcgcccaa ctgcgaaccc acgatatgcc cgtcggaatc cgcctgatgg cgaagcccgc
      781 ccagcgccgc gggccgctca tcgaacccgg gaagccatgg gagaccgtag ggatctccct
      841 ctcgaccatc attcgcgacg gtgccgtctt ccgcgcttgg ggcacctgtc agggggagaa
      901 gggccgcttc cagtgtgtct tcgagtccga tgacggcctc gactggcggc gtccggaggc
      961 cggcgtcgtc gaggtcgacg gcagcacggc caacaacctc atcggccccc cctggtgggg
     1021 cggcaccgtc ttcatcgatc cctcggctcc ggagaacgag cggtacaagg ccgtcaccga
     1081 gggcaggatc tcggccaacg acttcgaggc ctatcgcgag cggaggcccg gcgcctggga
     1141 gcgtcgtgcc cagcgggccg acgtgggtca gatcttcgct gtcctcggct cgatctcgcc
     1201 cgatggcctg cactggacca accagcccga gcccctcgtc gtcgagcaca gcgacaccca
     1261 gattgtcgcc cactacgaca ctcgccttcg gcagtacgtt atctacactc gcaactggtc
     1321 ggtgggcgag cgggacccct ccgcgcccga tgaccgaagg ctcagctggc tcagcgtggg
     1381 tcgccgctcc atcggccgga ccgcggc
//
LOCUS       DAOSPL010001415         1405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_93178_length_1405_cov_2.794074, whole genome shotgun sequence.
ACCESSION   DAOSPL010001415 DAOSPL010000000
VERSION     DAOSPL010001415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93178_length_1405_cov_2.794074"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PLQ54_20260"
     CDS             complement(<1..372)
                     /locus_tag="PLQ54_20260"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOS95655.1"
                     /translation="MYEYLPPVLRYGVLVALYLFLFAAYRALCRGTAPRFDESGPPRA
                     ASRPAVGTIVIVGAGSTSSVRVGQQVPLLTTNVLGRDPGCQVVVEDQFASKRHAAIDL
                     RDGVYVLADLGGTQAWRDQADQ"
     gene            complement(377..1042)
                     /locus_tag="PLQ54_20265"
     CDS             complement(377..1042)
                     /locus_tag="PLQ54_20265"
                     /inference="COORDINATES: protein motif:HMM:NF023818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HOS95656.1"
                     /translation="MGILTTVERSIRGLVEGAFGKAFGGRVHPAEIAKALLARMRETE
                     RPSGHRPIVANQYTIRLSRADFAAIEQIRPEVQAELLHLLRVTADSEDWDMPGPLRVG
                     WDPDPRLSEGQMFIEAEFVAGLPRGWLRVIRGPLEGREFALNQAETTIGRAPGNHIVL
                     QDSDVSRLHAVVHAVGDRFILEDRESTNGTYVNHKQVTHARLATGDLIELGGTILAFE
                     RGV"
     gene            1174..>1405
                     /locus_tag="PLQ54_20270"
     CDS             1174..>1405
                     /locus_tag="PLQ54_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005951551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOS95657.1"
                     /translation="MLGVKVAPSIICADFCRLGEELALLEAAGADLLHIDIMDGMFVP
                     NITFGPMIVEAMRNETDLVFDTHLMIVEPDRYI"
BASE COUNT          236 a          471 c          457 g          241 t
ORIGIN      
        1 ctggtcggcc tggtcccgcc aggcttgggt gccgcccagg tcggccagca catacacacc
       61 gtcgcgcagg tcgatggccg cgtgacgttt ggaggcgaat tggtcttcca ccaccacttg
      121 gcagcctgga tcccggccca ggacgttcgt ggtcagcagg ggcacttgct ggcccacgcg
      181 gaccgacgat gtcgagccgg cgcccacgat gacaatcgtc cccaccgccg ggcgagaggc
      241 cgcgcgaggt ggtcccgact cgtcgaaccg aggggccgtc ccacggcaca gcgcgcggta
      301 cgcggcgaat aggaacagat acagcgccac cagcactccg tagcgcaaca ccggcgggag
      361 gtactcgtac aactgactac accccccgct cgaacgccag tatggtgccg cccaactcga
      421 tgagatcccc ggtggccagc cgggcatgtg tgacctgttt gtggttcaca tatgttccgt
      481 tggtgctctc gcggtcctcg aggatgaagc gatcgcccac ggcatggacg accgcatgaa
      541 ggcgagagac atcggagtcc tgcagcacaa tatggttccc gggagcacgc ccgatggtgg
      601 tctcggcctg gttcagagcg aactcacgcc cctccagagg gccgcgtatc acccgcagcc
      661 acccccgagg cagccccgcc acgaactcgg cctcgatgaa catctggccc tctgagaggc
      721 ggggatcagg gtcccacccc acccgcagcg gcccgggcat gtcccagtcc tccgagtcgg
      781 ccgtcacccg gagcaagtgg aggagctccg cctgcacctc gggccggatc tgttcgatgg
      841 cggcgaagtc ggcgcgagag agccggatgg tgtactgatt cgcgacgatg gggcggtgac
      901 ccgaggggcg ttccgtctcc cgcatccggg ccaggagggc cttggcgatc tcggcgggat
      961 gtactcggcc gccaaatgcc ttgccgaagg ccccctccac caagcccctg atcgaccgct
     1021 ccactgttgt gaggatgccc actcgcggta ccgcccgagc cggcgcggcc tcaattgcgc
     1081 ctgattccgc ccctgtgcta ggattatacg cggatgggtt ggaccgctcc aagccgccgt
     1141 tgcgcttgag gcgtcgagct cgttcgcgcg ggggtgttgg gtgtgaaggt agcaccgtcc
     1201 atcatctgtg ccgacttctg ccgcctgggg gaggagctgg cgctcctcga ggctgcgggc
     1261 gctgaccttc tccacatcga catcatggat gggatgttcg tgccgaacat cacctttggc
     1321 cccatgattg tcgaggccat gcgcaatgag accgatctgg tgtttgatac gcatctcatg
     1381 atcgtcgagc cggaccggta catcg
//
LOCUS       DAOSPL010001416         1404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_93259_length_1404_cov_3.108970, whole genome shotgun sequence.
ACCESSION   DAOSPL010001416 DAOSPL010000000
VERSION     DAOSPL010001416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93259_length_1404_cov_3.108970"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PLQ54_20275"
     CDS             complement(<1..792)
                     /locus_tag="PLQ54_20275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95658.1"
                     /translation="MSQALLLLVLGMVGPGGLPARAQSAGGPVGAVVNGLEQRPRSAA
                     VLIGGQPYADVYFLADALGITAVADTATASMQLTYYGTSLSLANGATIGGETLQLMQS
                     NGVLYAPLGPVARALGMSAALGGDGILRIESPRPFPRQVRYRETTDQLLVVLELSGAV
                     PFAARQAGRELTLTLATSQPFAPAEVTTPTLDMRGNPKPATPPQSVKYEVGGVLGDQV
                     VVANLAGPVIRLAVQNRYEVPTQRIYTLADPPRIVPIESLLEEYDD"
     gene            complement(883..>1404)
                     /locus_tag="PLQ54_20280"
     CDS             complement(883..>1404)
                     /locus_tag="PLQ54_20280"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537777.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOS95659.1"
                     /translation="FTGSISIQLDIHCHPAGSRFLPLRSNIPAISEYVFSPVDPEFAS
                     RCKEWGGGLILGGSNYGQGSSREHAALGPRYLGIRVVVAKSFARIHWSNLVNFGILPL
                     VVEASTYDALEQGDEVEVCGVHSAVRSGGSVTLRLARTGAEFEARHHLTPAQAQILLA
                     GGRLNAARGASGA"
BASE COUNT          227 a          489 c          465 g          223 t
ORIGIN      
        1 gtcgtcgtac tcctcgagca gcgactcaat cggcacgatc cgtggcgggt cggccagggt
       61 gtagatccgc tgggtcggca cctcgtagcg gttctggacg gcgaggcgga tcaccggccc
      121 cgccaggttc gccacgacta cctggtctcc gaggactccg cctacctcgt acttcaccga
      181 ctggggcggt gtcgccggct tcgggttccc ccgcatgtcc agtgtggggg tcgtgacctc
      241 ggccggcgcg aagggttggc tcgtggccag agtcagtgtc agttcccggc cagcctgccg
      301 tgccgcgaac ggcaccgccc ccgagagctc cagcaccacc aggagctggt ccgtcgtctc
      361 ccggtaacgc acctgtctcg ggaatggacg tggcgactcg atccggagta tgccgtcacc
      421 gccgagggcg gcgctcatcc ccaacgcgcg ggccaccgga ccgagcgggg cgtacagcac
      481 gccattggac tgcatgagct ggagggtctc gccgccgatc gtggcgccgt tggccaggct
      541 gaggctggtc ccgtagtagg tcagctgcat ggaggccgtt gccgtgtcgg cgacggcggt
      601 gatgccgagc gcgtcggcca ggaagtagac atcggcgtag ggctgcccgc caatcagcac
      661 ggctgccgag cgggggcgct gttccagtcc gttcaccacc gccccgacgg ggccaccggc
      721 gctctgggcg cgtgcgggaa ggccgccagg gcccaccata cccagcacca gcaatagaag
      781 ggcttgactc atccgcatgt ggtgtatccc gagtggtcgc ctcggctgcc gcggctcacc
      841 cgtacctccc ccgacactaa gacccccggg gcgccacggg gttcacgcgc cactggcacc
      901 ccgagccgca ttgagccgtc cgcccgccag cagaatctgg gcctgggccg gcgtcaggtg
      961 atgccgggcc tcgaactcgg caccggttcg ggcgagccga agcgtcaccg agccgcccga
     1021 tcgtactgcc gagtgtaccc cgcacacctc cacctcatcg ccctgttcca gcgcgtcata
     1081 ggttgacgcc tcaacgacca gtggcagaat cccgaagttg accagattgg accagtgaat
     1141 gcgggcgaaa ctcttcgcga ctaccacccg gatccccaaa taccgcggcc cgagcgccgc
     1201 gtgttcacgg ctcgaacctt gaccgtagtt ggagccgccc agaatgagac ctccccccca
     1261 ctccttgcac cgggaggcaa actcgggatc caccgggctg aagacgtact ccgaaatggc
     1321 ggggatgttc gatcggagcg gcaggaagcg gctgcccgcg gggtggcagt gtatgtccag
     1381 ctggatgctt atgcttcccg tgaa
//
LOCUS       DAOSPL010001417         1402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_93451_length_1402_cov_2.677803, whole genome shotgun sequence.
ACCESSION   DAOSPL010001417 DAOSPL010000000
VERSION     DAOSPL010001417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93451_length_1402_cov_2.677803"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1402)
                     /locus_tag="PLQ54_20285"
     CDS             complement(<1..>1402)
                     /locus_tag="PLQ54_20285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95660.1"
                     /translation="EPAGDLLDRPAHVLQRVLDAFEARGFRYGISIGGATDEGYRAYR
                     IDERLVVRGAAGGEPASIALNYSPPRDGRALIVALDSVTGAVAHIGLEAIRIPLGVDT
                     AGGTVTFTVGKKGDGRTYDVFVTPESFVPSGEGVGDIWSNFDRRRARLLGHLSEISYG
                     PGFRFFLSPLCHDLSTSRSRDLPGTSTGMPEAFAEWLRGRYGDLQALARAWCAEPEPP
                     SFDAAGRLVPATHGAATTVMVDPVTMKCLTVRSAETRFARDLEEFREESVARCLDILC
                     ADIRSLVADVPVVVEHQWATRRYFTATDPRGGPDGVAMRSFGPAAELGVSDAAVTGGE
                     ASLGRHTLWCIASQTAPTAGGEARAAGYANATVMTTAFDTLLSCGARGLFVLGASLDG
                     IAGYVSHDMARQPEQLGWIAQYRSLLEKRIGALRQPRFVYVYPPAQRTPLLPQLGPSS
                     PFGLDGSLAGTPARLLP"
BASE COUNT          241 a          469 c          493 g          199 t
ORIGIN      
        1 gggcagaagg cgtgccggcg tgccggcgag gctcccatcg aggccgaagg ggctagacgg
       61 accaagttgg ggcagcaacg gcgtcctctg ggcgggcggg tagacgtaca cgaaccgggg
      121 ctggcgcagg gccccgatgc gtttctcgag gagcgatcgg tactgggcga tccagccgag
      181 ctgctcgggc tgccgcgcca tgtcgtggga gacatacccc gcgatgccgt ccagtgaggc
      241 accgagaacg aacagaccac gggcgccgca gctgagcaag gtgtcgaagg ctgtagtcat
      301 caccgtggcg ttggcgtacc ccgctgcgcg ggcctcgccg cctgccgtgg gcgccgtctg
      361 cgaagcgata caccacagcg tatgccggcc caggctagct tcaccacccg tcacggcggc
      421 atcgctcact ccgagctcgg ctgcgggccc gaagctcctc atggccacgc catcgggccc
      481 tcctcgggga tcggtggcgg taaagtagcg ccgggtcgcc cactgatgct cgaccacgac
      541 cggcacatcc gcgaccaggg agcggatgtc ggcacacagt atgtccagac agcgggcgac
      601 cgactcctcg cggaattcct cgaggtctcg ggcgaatcgc gtctcggcgg aacggaccgt
      661 gaggcacttc atggtgacag ggtccaccat gacggtggtc gctgcaccgt gcgtggccgg
      721 aacgaggcgg cctgcggcat cgaaactggg cggctcgggc tcggcgcacc aagcccgagc
      781 cagcgcctgg aggtctccgt agcgaccccg gagccattca gcgaatgcct caggcattcc
      841 cgtgctggtg ccgggcaggt cacgagagcg gctcgtcgat agatcatggc atagtgggga
      901 taggaagaaa cggaagcccg ggccatagct gatctccgac aggtgcccga gcaaccgcgc
      961 ccgtcggcgg tcgaagttgg accagatgtc gccgacaccc tctcccgatg gcacgaagct
     1021 ctcgggtgtg acgaacacgt cgtaggtccg gccgtccccc ttcttaccaa cggtgaaggt
     1081 cacggtgcca cccgctgtat cgacacccaa cgggatgcgg atcgcctcga ggccgatatg
     1141 cgcgaccgcc ccggtcacgc tgtcgagggc gacgatcagg gcccgcccgt cacgcggcgg
     1201 ggagtagttg agggcgatgc tcgcgggctc cccgccagcg gcgccgcgaa ccaccagtct
     1261 ctcgtcgatg cggtacgcgc ggtagccctc atcggtggcg ccgccaatcg agatgccgta
     1321 gcggaaaccc cgggcctcga aggcgtccag cactcgctgg agcacgtggg cgggccggtc
     1381 cagaagatcg cccgcgggct cg
//
LOCUS       DAOSPL010001418         1400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_93629_length_1400_cov_3.008178, whole genome shotgun sequence.
ACCESSION   DAOSPL010001418 DAOSPL010000000
VERSION     DAOSPL010001418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93629_length_1400_cov_3.008178"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PLQ54_20290"
     CDS             <1..352
                     /locus_tag="PLQ54_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014687432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOS95661.1"
                     /translation="VSGAVLAFRFFRGPYRRVELLQRRLGFAYTLLSRKLYIDELYDI
                     VIVGPAVGLSRLAADVTDTRGIDGIVHGAAAIAYRAGEGLRRLQTSHTRVYALAMLVG
                     AVALVLYLARGMLF"
     gene            404..>1400
                     /locus_tag="PLQ54_20295"
     CDS             404..>1400
                     /locus_tag="PLQ54_20295"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOS95662.1"
                     /translation="MGLTLEFGFPVLSTLVFLPALGALVCLLIPRERAGALRAVPLVF
                     AVATGLLALLISLGFEPRAVGSETQVAYQFAERMPWIGPLGVEYSIGLDGISMLMVLL
                     TALLGIVAVLASWTSIEHRIKEYMICLLLLETAMLGVFVALDLVLFYVFWESMLIPMV
                     LIIGAWGGPRRVYAAVKFFIFTMFGSVLMLVGILWLHHLTGTFSIPELAARAPAAVAA
                     SQGELLFLLFGIAFAIKVPLVPLHTWLPDAHTEAPTAGSILLAGVLLKMGAYGFLRMC
                     VPFFPAATVAFAPALVWLGVIGILYGGACALAQRDVKRLVAYSSVSHLGFVILGVF"
BASE COUNT          174 a          483 c          443 g          300 t
ORIGIN      
        1 cgtgtcaggc gccgtgctgg ccttccggtt cttccgcggg ccgtaccggc gtgtcgagct
       61 gctgcagcgc cggctcgggt tcgcatacac actgctgagc cgaaagctgt acatcgacga
      121 gctgtatgac atcgtcatcg tcggaccggc ggtgggcctc agccgcctgg ccgcggacgt
      181 cacggatacc cgcggcatcg acgggatcgt ccacggcgcg gcagccatcg cgtaccgcgc
      241 cggagagggt ctgcgacgcc tccaaaccag ccacacccgc gtctacgcac tggccatgct
      301 ggtgggggct gtcgcactgg tcctgtatct cgcgcgtggc atgctgttct agtccggctc
      361 gcctggcgct gccggcctgg actcgcacgg aaaggacgac ggcttggggc tgaccttgga
      421 gttcggattc ccggtgctct cgacgctggt gttcctgccg gcgctcggag ctctggtatg
      481 cctgctcatc cctcgtgagc gcgcgggcgc cctccgggcc gtcccccttg tgttcgctgt
      541 cgctaccggc ttactggccc tgctcatctc gctcgggttc gagccgcggg ccgtcggcag
      601 cgagacgcag gtcgcatacc agtttgccga gcgaatgccg tggatcgggc ccttgggcgt
      661 cgagtactcc atcggcctgg atggtatcag catgctgatg gtcctgctca cagcactgct
      721 gggcatcgtc gccgtcctgg cctcctggac cagtatcgag caccggatca aggagtacat
      781 gatctgcctg ctgctgctcg agaccgccat gctgggtgtg ttcgtcgccc tggacctggt
      841 gctgttctac gtcttctggg agtccatgct gatccccatg gtcctgatca tcggcgcctg
      901 gggcggtcct cggcgcgtct atgccgcagt gaagttcttc atcttcacga tgttcggcag
      961 cgtgctgatg ctggtcggca tcctgtggct ccaccatctc acgggaacct tctccatacc
     1021 cgaactcgcc gcccgcgccc ctgccgcagt ggcggcatcc cagggcgagc tcctgttcct
     1081 gctgttcggc atcgccttcg ccatcaaggt ccctctcgtg ccactgcaca catggctccc
     1141 ggacgcccac accgaggcac cgactgccgg cagcatcctg ctggccgggg tcctgctcaa
     1201 gatgggggcc tatggcttcc ttcgcatgtg tgtcccgttc ttccccgcgg ccaccgtggc
     1261 attcgccccg gccctggtgt ggctgggcgt catcggcatc ctctatggcg gcgcctgtgc
     1321 gctggcccag cgcgatgtca agaggcttgt cgcgtactcg agcgtcagcc atctcggatt
     1381 cgtcatcctc ggtgtgttcg
//
LOCUS       DAOSPL010001419         1399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_93758_length_1399_cov_2.479167, whole genome shotgun sequence.
ACCESSION   DAOSPL010001419 DAOSPL010000000
VERSION     DAOSPL010001419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93758_length_1399_cov_2.479167"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            650..>1399
                     /locus_tag="PLQ54_20300"
     CDS             650..>1399
                     /locus_tag="PLQ54_20300"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOS95663.1"
                     /translation="MRLYAGVVRFFVIVVLAHAAMFAVVGALASRSRGEEIPTNTVRL
                     AGSSLQVRTLDPVGMGDTSSTSICDQIYEGLVSYDQETLAVVPCLADRWTVSPDGLTY
                     TFHIRPGVRFIDDPCFPGGHGREVAASDFVFNFTRMADPVAAGTGFWLVDGRVVGATE
                     YNEARRRLIEATPRLWETDSIFEGTRGIPGYQAPAPDTFQIRLERPFAPFLKVLAMAY
                     FRVAAPEAVEMYGPARNAPGRDTFFSHPVGTG"
BASE COUNT          215 a          463 c          471 g          250 t
ORIGIN      
        1 tcttgaccga tttgaccgat tgaccgaact ttgcacaact tcccctagaa tcacgccatc
       61 ggctctcaca cgcgctatgg ggaagttttg tggaacatcg gtcaatcggt caaatcggtc
      121 aagacccacg gggcctgcca gtgcgatgga cgaacatttg cgcttgacat gggaacacaa
      181 atcgtgtata atagtatcat tgctggtcag gagacgctct ggcctccggg tgcgggctga
      241 gacgtatcgg ccgtcccccg gcaccgaggc catgccgatg gagcgaggac cctcctgttg
      301 ccagaaggcg cggtcgagcc cagcacttgg ggaccgtgtg cgcgtcgagg tcggatggcg
      361 gggtagacgc aaccgccagc cgtggcggcg gaggtcggac gatcgtgagt gagcgctcag
      421 ctcgcctggt gcaggcgggc tgcgcgtacg tcgggcgctg ccatgcgccc acggctgtgc
      481 accctcgagt gacgcgaggg tccgcgactt tccccagaat cccgcctcgc actggcgcgc
      541 gcgatggggg cttatgagaa catgcgtcac ttgtgtcact tggatcaccc gacaggatgc
      601 ggccccctga tggcgaatat ggacaggttt gcggaatcgg gaggccggtg tgaggctcta
      661 cgcgggtgtg gtgcggttct tcgtcatcgt ggtgctggct catgccgcca tgttcgccgt
      721 ggtcggcgcc ctggcgagcc gctcccgtgg cgaggagatc cccaccaaca ccgtccggct
      781 cgccggcagc tcgctgcagg tgcgcacgct cgaccccgtc ggcatgggcg acacgtcgtc
      841 gaccagcatc tgcgatcaga tctacgaggg cctggtctcc tatgaccagg agaccctggc
      901 cgtggtgccc tgcctggccg accggtggac cgtctcgccc gatgggttga cctacacgtt
      961 ccacattcgc cccggcgtgc ggttcatcga cgacccgtgc tttcccggcg gccacgggcg
     1021 cgaggtggcc gcgtccgact tcgtgttcaa cttcacgcgc atggccgacc cggtggcggc
     1081 gggcacgggc ttctggctgg tcgatgggcg cgtggtgggc gccaccgagt acaacgaggc
     1141 gcgccgccga ctgatcgagg ccactccccg gctgtgggag accgactcga tcttcgaggg
     1201 cacccgcggg atccccggct accaggcccc ggcgcccgac accttccaga tccggctcga
     1261 gcgaccgttc gcccccttcc tgaaggtgtt ggccatggcc tacttccgtg tggcggcgcc
     1321 cgaggccgtc gagatgtacg ggcccgcccg caacgcgccg gggcgcgaca cattcttcag
     1381 ccatcccgtc ggcaccggg
//
LOCUS       DAOSPL010001420         1398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_93858_length_1398_cov_3.107967, whole genome shotgun sequence.
ACCESSION   DAOSPL010001420 DAOSPL010000000
VERSION     DAOSPL010001420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93858_length_1398_cov_3.107967"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(241..>1398)
                     /locus_tag="PLQ54_20305"
     CDS             complement(241..>1398)
                     /locus_tag="PLQ54_20305"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOS95664.1"
                     /translation="PSYPEKGGQIGDRGLIRGPRGTFRVEDTQEPVDGVIIHSGVVDN
                     GEIARDDIVTAEPDLMRLNAIRRAHSATHLLHQALRTVLGTDVAQAGSLVEPDRLRFD
                     FACPTAPSADDLLRVERVANEQILADLPVTVVETDRKTAESMGAVALFGEKYGAVVRV
                     VSMGEFSRELCGGCHVPSTARIGQLKLLNSASIGAGVRRVEALTGLEALGHAASMEAL
                     VRDVALRINCRGEELPNRVAALMEQVKDLRRQLAESQKRLPTSVDPGALLSEAKQLAP
                     GVRLVAHAADGLGPDALRDLADRLTESHGVIALLACPQSPDRVAIVCKLSKDLAGKAH
                     AGELVGAAAKAMNGGGGGNPHFAQGAGRDATLIAQGLAAAEEAATAQLRRP"
BASE COUNT          208 a          483 c          478 g          229 t
ORIGIN      
        1 aacgagtagt atgagtgcca tcctcatgtc gcagctctcc ccgcagccac tgtgcgaggg
       61 caccacgttc gcgccaagcg ctatcgcgtg cccccatgcc tgactcctct aggcagtggc
      121 gagctgttcc ggcccgtggc gcgcgaatcc ttcgcgggtc gctatgcaac tgcgggctgc
      181 gccccggccg agtgctggcc ggggcccgga gtgagtccgc gccggtcgac gcggatcgga
      241 ctacggccgg cgcagttgcg cggtggccgc ctcctcggct gcggcgagcc cttgggcgat
      301 gagggtggca tcgcgcccgg cgccttgggc gaagtgggga ttcccgccgc cgcccccgtt
      361 catggctttg gccgccgcgc ccaccagctc tccggcgtgg gccttcccgg ccaggtcctt
      421 cgagagcttg cacacgatgg ccacccgatc cggcgactgc ggacaagcca gcagtgcgat
      481 cacgccatgg ctctcggtca ggcggtccgc caggtcgcgc agcgcgtcgg gtccgaggcc
      541 atccgccgcg tgggcaacta gccggacccc gggggcgagc tgcttcgcct cggagagcag
      601 cgctcccgga tccacggagg tgggaaggcg cttctgagac tcagcgagct gacgccgcag
      661 atccttgacc tgctccatca gtgccgccac gcggttgggc agctcctcgc cgcggcagtt
      721 aatgcgcagt gcgacgtcgc ggaccaatgc ttccattgag gccgcgtgcc caagggcctc
      781 caggcccgtc agcgcctcga cacgcctcac tccggctcca atgctggcgg agttgagcag
      841 tttgagctgg ccgatgcggg ccgtgctcgg tacgtggcat ccaccgcaca gctcgcgcga
      901 gaactcgccc atgctcacga cccgaaccac ggcgccgtac ttctcaccaa acaatgcaac
      961 ggcgcccatc gactcggcgg tcttgcggtc ggtctcgacg acggtcacgg gtagatccgc
     1021 gaggatctgc tcattcgcga ctcgctcgac acggagcagg tcgtccgccg agggagcggt
     1081 ggggcaggcg aagtcaaacc ggagtcggtc gggttcgacc agtgagccgg cttgggccac
     1141 atcggtcccc aggacggtgc gcagggcttg atggagcaga tgagtggcag agtgcgcccg
     1201 ccggatcgcg ttcagacgca tcagatcggg ctcggccgtc acgatgtcgt cccgggcgat
     1261 ctcgccgttg tccacaacgc cggagtggat gatcacacca tcgacaggtt cctgggtgtc
     1321 ctcgacccgg aacgtgcctc tcgggccacg gatgaggcct cggtcgccga tctgaccgcc
     1381 tttctcgggg taggaggg
//
LOCUS       DAOSPL010001421         1397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_93955_length_1397_cov_3.318927, whole genome shotgun sequence.
ACCESSION   DAOSPL010001421 DAOSPL010000000
VERSION     DAOSPL010001421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93955_length_1397_cov_3.318927"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1065)
                     /locus_tag="PLQ54_20310"
     CDS             complement(190..1065)
                     /locus_tag="PLQ54_20310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95665.1"
                     /translation="MTARRCPALAAAALLPILASHVFAAYENVLPGVRDGLKAFAADG
                     KVRHCVIRVKVEAQGQTREQPDQEIWYQGDDRLRADLSTQNFVLAVSPEGGTLFSAQA
                     GLALVARGEAFEKLGESAPDRQRNLGVNPPLCVLDRALAEEATLSIERESAFDGRDCW
                     VMTVAPERLEAWSKIILGEQPVTLSAVHIGVDKQDFLVRGLDFTGTQFAPNGGPTTDF
                     KVTLAYLGAENGVEVPESFFALELPEDAEVMEWRAGAKTEEMLVEFVQAVVRRLEEQG
                     KAQIDDEAAGQEPPQ"
BASE COUNT          191 a          494 c          476 g          236 t
ORIGIN      
        1 tggtgaaacg ggggtgtggc tcgggggcga cgggcccggt gggggtcccg cccagcaggg
       61 ccagggtcga tccggatcca gacgggctca tggtgtgcat ccctccgcga aggcgccggg
      121 aatgctaccc gggccgatgg tatgtgcgga gcgggcgccg cgagcacctt ctggtggggc
      181 gaagccagct tactggggtg gctcctggcc ggccgcctcg tcgtcgatct gggccttccc
      241 ctgctcctcc agccgccgga ccacggcctg gacgaactcc accagcatct cctcggtctt
      301 ggcgcccgcc cgccactcca tgacctcggc gtcctcgggt agctccaggg cgaagaagct
      361 ctcgggcacc tcgacaccgt tctccgcccc tagatatgcg agtgtcactt tgaagtcagt
      421 cgtgggtccc ccgttgggtg cgaactgggt gccggtgaag tcgagaccgc gcaccaggaa
      481 gtcctgcttg tccacgccga tgtgaaccgc cgacagcgtc acgggttgct cgcccaggat
      541 gatcttgctc caggcttcga ggcgctcggg agcgacggtc atcacccagc aatcgcgtcc
      601 gtcgaaggcg ctctcgcgct cgatcgacag cgtcgcctcc tcggccaagg cgcggtccag
      661 cacgcacagc ggcgggttga cgcccaggtt gcgttgccga tcaggcgcgg actcgcccag
      721 cttctcgaag gcctcgcccc gggcgaccag cgcgagcccc gcttgcgcgc tgaacaacgt
      781 gcccccctcg ggcgacacgg ccaggacaaa gttctgggtg gacagatccg cgcgcaggcg
      841 gtcgtcgccc tggtaccaga tctcctggtc gggctgttcc cgggtctggc cctgcgcctc
      901 gaccttgacc cggatgacgc agtgccggac cttgccatcg gccgcgaagg ccttaagccc
      961 gtccctgact cccggcagga cattctcgta ggccgcgaag acgtggctcg cgaggatggg
     1021 aagaagagcc gcggccgcca gggccggaca gcgtcgagcc gtcatgaggt gttcccctct
     1081 ccgatgcccg tgcagacgac cggcagggct cccggttgcg gtggcgattc cgtccagccc
     1141 cattgtacag cctgcgagtg cgggtgtgcg ggccgtacgg gtcaaggacc ggtcccctcg
     1201 gccggggaat cagctccacg gaggtgccgc catgcttcgt ttcctcgccg cggcctcatg
     1261 cctggccgcc gcgccgccgg tctgcgccgc cgtctatcac ctgccttcgg ctgaggtcga
     1321 ggtcaccgct gagtccaagg tcgtcgtcca ctctgccggc gcgagcttcc ggccgctgac
     1381 cggtctgggg ccggtct
//
LOCUS       DAOSPL010001422         1397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_93966_length_1397_cov_3.040238, whole genome shotgun sequence.
ACCESSION   DAOSPL010001422 DAOSPL010000000
VERSION     DAOSPL010001422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93966_length_1397_cov_3.040238"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PLQ54_20315"
     CDS             complement(<1..404)
                     /locus_tag="PLQ54_20315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95666.1"
                     /translation="MPEDEATPKPAPTTHDPSATHDDEAENVWVRSREVGIIGDRGLL
                     PRESVGGFGGLALFIVFVVGAAAGGLVLWALKGPALTEAEEAAAFYEARTQVLLSAAA
                     EGALEQVKRARTEVAARNFQAAQESIEAAEGLV"
     gene            complement(578..>1397)
                     /locus_tag="PLQ54_20320"
     CDS             complement(578..>1397)
                     /locus_tag="PLQ54_20320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95667.1"
                     /translation="GSTRTVSLNLRNSLDVGWNLNVFAVSSPHVRVETPHLREHVPRR
                     GSKATSLTLTCEPDAREGEAESLRLVVAASDVFGKIPEASMRVLPANDAAGVIGTLPA
                     PVRDALRDYAMPTTHIHDVRRAAFELLDLLYVCDETPRPGTAEAVERAARSGCVVVCL
                     GPCVPALFESDAHLRATLGAGSARVGAAMDAGHQVLCVPHALRRDRLTAIGWLEHLGA
                     AWETVLADETGRPVLASASLEPGALIVALVSTEGDEGVRLGRNLVEWAFQVAGI"
BASE COUNT          213 a          494 c          477 g          213 t
ORIGIN      
        1 accaggccct cggccgcctc gatggattcc tgagcggcct ggaagttccg cgccgccacc
       61 tcggtgcggg ctcgcttcac ctgctccagg gcgccctcgg cggcagcgct cagcaggacc
      121 tgagttctcg cctcatagaa cgcggcggct tcctcggctt cggtgagtgc tggacccttc
      181 agcgcccaca agacgagtcc gccggcggcg gccccgacca cgaacacgat gaacagcgcc
      241 aggccgccga agcccccgac ggattcgcgg gggagcaggc cgcggtcgcc aatgatgccg
      301 acctcgcgcg atcggaccca tacgttctct gcctcatcgt cgtgggtggc ggaggggtca
      361 tgggttgtcg gggcaggctt gggagtcgct tcgtcttcag gcatattcag cccccactca
      421 ctgatatctc gcagcgcaat gggcgcgggt atcatagaca gccgcttagc tgagcgtcaa
      481 ccggactcgc cgggctccga cgggtctgga gcgcttcccg atgcgaactg cgacggggga
      541 ggtggctgtt cgcgcactct cccatgccag gtatgcccta gatgccggcg acttggaagg
      601 cccactcgac gagattgcgt cccagccgca cgccttcgtc gccctcggtc gagaccagtg
      661 ccacgatgag ggccccgggc tcgagcgatg cgctggccag caccggacgg ccagtctcgt
      721 cggcgagcac cgtctcccat gccgccccca ggtgctcgag ccagccgatg gccgtcagtc
      781 ggtcgcggcg taacgcgtgg ggcacgcaga ggacctggtg ccccgcgtcc atcgccgcgc
      841 ccacgcgggc cgacccggcg cccaaagtgg cgcgcagatg cgcgtcggat tcgaacaggg
      901 ccggtacgca ggggccgaga cacaccacca cgcagccgct gcgggccgcg cgctccacgg
      961 cctcggcggt gcccggccgt ggggtctcgt cgcacacgta gagcaggtcg agcagctcga
     1021 aggccgcgcg gcgcacatcg tgaatgtgcg ttgtgggcat ggcgtaatca cgcagcgcgt
     1081 cgcgcacggg cgctggcaat gtgccgatga cccccgccgc atcgttcgcc ggcagcacgc
     1141 gcatggaggc ttccgggatc ttgccgaaga catccgacgc cgcgaccacc aggcgcaggg
     1201 actccgcttc cccctcccgc gcgtccggct cacacgtgag tgtcagagac gtggccttgc
     1261 tgccccggcg cggaacgtgt tcccgcagat ggggcgtctc gacccgcaca tgggggctac
     1321 tcaccgcgaa cacgttgagg ttccagccca cgtccaggct gttgcggagg ttcagggaga
     1381 cggttcgggt ggatccg
//
LOCUS       DAOSPL010001423         1397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_93978_length_1397_cov_2.706408, whole genome shotgun sequence.
ACCESSION   DAOSPL010001423 DAOSPL010000000
VERSION     DAOSPL010001423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93978_length_1397_cov_2.706408"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="PLQ54_20325"
     CDS             complement(<1..1163)
                     /locus_tag="PLQ54_20325"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOS95668.1"
                     /translation="MSLAHSSPLRGEDCLIQPLPLNGRDAADLFAAIRHEPCPWLLET
                     LGQPGPSFLGSRPRRVLVSGQGELEPPPPGVTVTRVSEDPLRAARTVLAESPARVVQP
                     GLAFAGGLVGFFGYDTAHCFEKLPCTTVDDLRLPDWILMETRYVVAIDTARDEAQIVV
                     PILRGGTEDEARRELVCLYDQLATAPKRVKPTVARGGAITIPYTSTYTPEDYASMVLR
                     VKEYIAAGDIIQANVSQRLAVPTRADAFALYEHLRAVNPSPFAFYFDFGSFQRASCSP
                     ERLLEVRGRRVQTRPIAGTRPRGADAHSDEALRADLLLSEKERAEHIMLVDLERNDLG
                     RVCEYGTVRVSELMVVEAYSHVFHIVSNVVGRLRNERDALDAVAAAFPGGTITG"
     gene            complement(1144..>1397)
                     /locus_tag="PLQ54_20330"
     CDS             complement(1144..>1397)
                     /locus_tag="PLQ54_20330"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS95669.1"
                     /translation="ECLDVDAIHARFGTRLTFDGTIGTQSTMPWGTPRQVRERVKEVV
                     TRYGESGGLIIGPTHVLEPEVPVENIVALADACREFGTF"
BASE COUNT          220 a          439 c          513 g          225 t
ORIGIN      
        1 ccggtgatgg tcccgccggg gaacgcggcc gcgaccgcgt cgagcgcgtc gcgttcgttc
       61 cggagccgtc cgacgacgtt ggagacgatg tggaagacgt gggagtaggc ctcgacgacc
      121 atcaactcgc tcacccgcac ggtgccgtat tcgcagacac ggccgaggtc gtttcgttcg
      181 aggtcgacca gcatgatatg ttcggcgcgt tccttttcac tgagcagcag atcggcgcgc
      241 agtgcttcat cggagtgggc gtcggcgccg cggggccgcg ttccggcgat gggccgagtc
      301 tgtacccggc ggccccggac ctccagcagc ctctcggggg agcaactggc acgctggaag
      361 cttccgaagt cgaagtagaa cgcgaatggc gaggggttca cggcccggag gtgctcatag
      421 agcgcgaagg cgtcggcccg ggtcggcacg gccagcctct gcgatacgtt ggcctggatg
      481 atgtcgccgg cggcgatgta ctccttcacg cggagcacca tgctggcata gtcctcgggt
      541 gtgtatgtgg acgtgtaggg gatggtgatg gcccccccgc gggcgacggt gggcttcaca
      601 cgtttgggtg cagtggccag ctgatcatac aggcagacca gttcgcggcg ggcttcgtcc
      661 tccgtccctc cccggagtat ggggaccacg atctgtgcct cgtcgcgagc cgtgtcgatc
      721 gcgaccacat agcgggtctc catcaggatc cagtcgggca ggcgcaggtc atccaccgtg
      781 gtgcacggca gcttttcgaa gcagtgggcg gtgtcgtagc caaagaaccc cacgaggccg
      841 cccgcgaacg ccaggccggg ctggacgact cgcgccggcg actcggccag cactgtccgg
      901 gcggctcgga gtgggtcctc ggagacgcgg gtcacggtga cgccaggggg cggtggttcg
      961 agctcgccct gaccgctgac gagcaccctg cggggccgtg agccgaggaa agacggcccg
     1021 ggctgcccta gtgtctcgag cagccacgga cacggctcgt ggcggatggc ggcgaaaagg
     1081 tcggccgcgt cgcggccgtt cagcggcagg ggctggatga gacagtcttc gccgcggagc
     1141 gggctagaat gtgccaaact cacggcaggc atccgccagg gcgacgatgt tctcgacggg
     1201 cacctcaggc tcgagcacat gggtggggcc gatgatcagg ccgccgctct ccccgtagcg
     1261 ggtgacaacc tccttgacgc gttcccgcac ctgccgagga gtgccccacg gcatggtgga
     1321 ctgggtcccg atggtgccgt cgaaggtcag ccgcgtgccg aagcgggcgt ggatggcatc
     1381 cacgtccagg cactcgg
//
LOCUS       DAOSPL010001424         1394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_94269_length_1394_cov_3.026886, whole genome shotgun sequence.
ACCESSION   DAOSPL010001424 DAOSPL010000000
VERSION     DAOSPL010001424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94269_length_1394_cov_3.026886"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="PLQ54_20335"
     CDS             complement(<1..1028)
                     /locus_tag="PLQ54_20335"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95670.1"
                     /translation="MASPGGGPVLYFIPHTHWEGAVFKTRDQYLDMGLPNILRALALL
                     EAHPNFRFTLDQVCYVRPFLERFPEEAARFRQFVREGRLGIVGGTDVMLDVNMPGGES
                     FIRQVLYGKRFFRQKLGVDVTVCWQLDTFGHHAQMPQLLAQAGFRSFWFFRGVPSWET
                     PAEFLWEGIDGTRVPAYWLPHGYALSYGSPATVPEFGRFVDERYHMLDPFSKRDRRVA
                     PGGADVCEPEGHLPAVAAAHNAQPDIAVAVELATPQEYEAAVPIDDTFPVVTGELNPI
                     FQGIYSSRIELKQWTRELESALTTAEKLGVLLGSLGEPVADGDLGGAWGPMLFNQAHD
                     LMSGVMTD"
     gene            complement(1057..>1394)
                     /locus_tag="PLQ54_20340"
     CDS             complement(1057..>1394)
                     /locus_tag="PLQ54_20340"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOS95671.1"
                     /translation="AGCGPFDWTIVHEVSPAQLQALMDDGLPLQIIDVRTPAEFAAGH
                     IHGSVNRPLADLDTWAAGIDTPTRICVVCQIGARSATAAEALVARGFSQVYALAGGLA
                     AWPGQLVTS"
BASE COUNT          232 a          463 c          498 g          201 t
ORIGIN      
        1 tggtcggtca tcacgcccga catgagatcg tgggcctggt tgaacagcat gggcccccac
       61 gcacccccca aatccccgtc ggcaaccggc tcgccgagcg agccgagcag cacgccgagc
      121 ttctccgccg tggtgagggc cgattcgagc tcgcgcgtcc actgcttgag ctcgatgcgg
      181 ctgctgtaga tgccctggaa gatggggttg agctcgccgg tgaccaccgg gaacgtgtcg
      241 tcgatgggca cggccgcctc gtactcctga ggcgtggcga gctccaccgc caccgcgatg
      301 tccggctggg cgttgtgcgc cgcggccacg gccggcaggt gtccctcggg ctcgcagaca
      361 tccgcgccgc ctggcgccac ccggcggtcg cgcttcgaga aggggtccag catgtggtag
      421 cgctcgtcga cgaaccggcc gaactcgggc accgtggccg gcgagccata gctcagcgca
      481 tagccatggg gcagccagta ggcggggacc ctggtgccat cgatgccctc ccacaggaac
      541 tcagccggcg tctcccagct cgggacgcct cggaagaacc agaacgaccg gaaccccgcc
      601 tgcgccagaa gctgcggcat ctgcgcatgg tgcccgaagg tgtcgagctg ccagcagacg
      661 gtcacatcga ctccgagctt ctggcggaag aaccgcttgc cgtacagcac ctggcggatg
      721 aaggactcgc cgcccggcat gttgacatcg agcatgacat cggtgccgcc cacgatgccg
      781 agccggccct cgcggacgaa ctggcggaag cgcgccgcct cctcggggaa cctctcgagg
      841 aacggccgca catagcagac ctggtcgagg gtgaaacgga agttcggatg ggcctcgagc
      901 agcgccaggg cccggaggat gttgggcaag cccatgtcga ggtactggtc gcgggtcttg
      961 aagaccgcgc cctcccagtg ggtgtgcggg atgaagtaga gaaccggtcc gccgccaggg
     1021 ctggccatgg ggtgagcacc ttctgtcggg gatgagctag gaggttacca gctggccggg
     1081 ccaggccgcg aggccgccgg cgagggcgta gacctggctg aatccgcggg caacgagggc
     1141 ctcggccgca gtggcgctcc gcgcaccgat ctggcagacg acgcagatcc gcgtgggcgt
     1201 gtcgatgccc gcggcccatg tatccaggtc ggccagtggc cgattcaccg agccgtggat
     1261 atggccggcg gcgaactccg cgggcgtgcg gacgtcgatg atctgcaagg gcagtccgtc
     1321 atccatcagg gcctggagct gggcgggcga cacctcgtgc acgatcgtcc agtcgaaggg
     1381 tccgcagccc gcgg
//
LOCUS       DAOSPL010001425         1393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_94355_length_1393_cov_3.360987, whole genome shotgun sequence.
ACCESSION   DAOSPL010001425 DAOSPL010000000
VERSION     DAOSPL010001425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94355_length_1393_cov_3.360987"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1393
                     /locus_tag="PLQ54_20345"
     CDS             <1..>1393
                     /locus_tag="PLQ54_20345"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HOS95672.1"
                     /translation="AWGYFGPWMGVEKLQKHWRYLVARYGALPVVWCVAGEANLPWYL
                     APGFPYDDREQVHQWTEVARYLKRVDPFHRPLSIHPTGLGQLSARGAIDDPALLDFDM
                     LQTGHGGREVLEPSIRTLRWSYDADPRMPVLNSEVCYEQLLGWIPADVQRLIFWASML
                     SGAAGQTYGANGIWQVNRRDQPHGASPHGGNYGTIPWDEAMNLPGSSQLALGKRLLER
                     YPWQRFEPHPEWASYGEGTTLSLDGCRWIWFPEGNPAEDAPVEHRYFRISFDLPEGQT
                     AAATLRMSADDACTAYLNGDPVGEARSWRTGAQIGSLAARLRPGRNVLAIRAENGQAP
                     VAKNPAGLIARLEIRLANGEIRRIDSGPQWRCAETETPGWAFLDFDDAALVSAMDLGP
                     YGMAPWGTLADHGPYEVPYAAGIAGQVRVIYMPQQRATTIHDLDDGVSYLARCVDPVT
                     GAETELGQVHPANG"
BASE COUNT          224 a          515 c          451 g          203 t
ORIGIN      
        1 gcctggggct actttggtcc ctggatgggt gtcgagaagc tccagaaaca ctggcgctac
       61 ctggtcgcgc ggtacggtgc cctgccggtt gtctggtgcg tggccgggga ggcgaacctg
      121 ccctggtacc tcgcgcccgg attcccctat gacgaccgcg agcaagtcca tcagtggacc
      181 gaagtcgccc gctatctcaa gcgcgtggat cccttccacc gcccgctctc gatccacccg
      241 acgggcctcg ggcagctcag cgcccggggc gccattgacg accccgcgct gctcgacttc
      301 gacatgctcc agaccggcca cggtggccgc gaggtgctcg agccctcgat caggaccctc
      361 cggtggtcct acgacgccga cccgcgcatg ccagtgctca acagcgaggt ctgctatgaa
      421 cagctcctcg gctggatccc ggccgacgtg cagcggctga tcttctgggc cagcatgctg
      481 tcgggcgcgg cgggacagac ctatggcgcc aacggcatct ggcaggtcaa ccgcagggac
      541 cagccccacg gcgcatcgcc gcatggcggc aactacggca cgatcccgtg ggatgaggcg
      601 atgaacctgc ccggatcctc gcaactcgcg ctcgggaagc gcctcctcga gcggtacccc
      661 tggcaacgct tcgagcccca tcccgaatgg gcgtcctatg gcgagggcac caccctcagc
      721 ctcgacggct gccggtggat ctggttcccc gagggcaacc cggccgagga cgccccggtc
      781 gagcaccggt acttccgcat cagcttcgac cttcccgaag gccagacagc cgcggccaca
      841 ctgcggatgt cggccgacga tgcgtgcacg gcctacctca acggcgaccc agtgggcgag
      901 gcccgatcgt ggcgcacggg cgcacagatc ggcagcctgg ccgcacgcct acgccctggc
      961 cgcaacgtcc tggccatccg tgccgagaac ggccaggcgc ccgtcgcgaa gaacccagcc
     1021 gggctaatcg cccgcctcga gatccgcctc gccaacggcg agatccggcg catcgactcc
     1081 ggcccgcagt ggcgctgcgc ggagaccgag acgccgggct gggcgttcct cgacttcgac
     1141 gatgcggcac tcgtttctgc catggacctg gggccgtacg gcatggcgcc gtggggcacg
     1201 ctcgccgatc acggccccta cgaagtgccc tacgccgcag gcatcgccgg gcaggtgcgg
     1261 gtcatctaca tgccccagca acgggcgacg accatccacg atctcgacga cggcgtgagc
     1321 tacctggctc ggtgtgtcga tcccgtcacc ggagccgaga ccgaactcgg ccaggtccac
     1381 ccggcgaacg gtt
//
LOCUS       DAOSPL010001426         1393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_94358_length_1393_cov_3.204783, whole genome shotgun sequence.
ACCESSION   DAOSPL010001426 DAOSPL010000000
VERSION     DAOSPL010001426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94358_length_1393_cov_3.204783"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..>1393)
                     /locus_tag="PLQ54_20350"
     CDS             complement(160..>1393)
                     /locus_tag="PLQ54_20350"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOS95673.1"
                     /translation="MGSTRSPMPPCGSTGYSPWPVTTGEKPKVYFLTGHGEYPVDPQG
                     GMGDRAAWSDVKTVLTDQQYEIKTLNLISQIPRSTTTLEELTSGASTRTGETKTEDGG
                     KASGSGDEAKKDSSPVVKVPDDCAALVIAGAKTALAPEEEQAIKDYLSNNGHAVICID
                     VGTGTDVAREKKLTAPDFNGILSAYQIRVGDEIVVEQVQDPFRGASLSFEALVQDFDE
                     SHPVAEGLTTVLLPACRPVYYEGQDQSQPNPMGGPPPQSPGKSIMKTPAGAWAERNAI
                     VPRGTRIQKDPGETGGPVSVAVAYGKEPGETEQPTMPGMPPQPAGETPRIVVFGSAQA
                     FTDGVTGVPQLSNLAAITKAIAWCSGRGSLVSIPPKSVEKRTLTVEKRQASVMRLVHI
                     LIGLGVLGSGVYVWWRRR"
BASE COUNT          210 a          457 c          455 g          271 t
ORIGIN      
        1 tcgtcagtgt cgccgtcgtg cccatgtcga tgtacttgac gtcgaagttg aggatctggc
       61 cctcaacagg tttctcgccg cgctcaaaaa cggccacata ggcaccgagg cccagcagga
      121 tgaccagggc gacaacagtg tgcttggtct tcatcggccc tatcgcctcc gccaccacac
      181 gtagacgccc gagccgagga cacccaaacc gatcaggatg tgcaccagcc gcatcacgct
      241 cgcctggcgc ttctcgaccg tcagggtgcg cttctcgaca ctcttgggag ggatactgac
      301 cagactgccg cgcccactac accaggcaat ggctttggtg atcgcggcca gattcgagag
      361 ctgggggacg cccgtcacgc cgtcggtgaa tgcctgggcc gatccgaaga cgacgatcct
      421 gggcgtctcg cccgccggct ggggtggcat gcccggcatc gtcggctgct cggtctcccc
      481 gggctccttc ccgtacgcaa cggccacaga aacggggccc cccgtttcgc ccggatcctt
      541 ctggattcgg gtgccacgcg gcacgatggc gttccgctcg gcccatgcgc cagcgggcgt
      601 cttcataatc gacttaccgg gcgactgcgg cggcggccct cccatggggt tgggctggct
      661 ttggtcctgc ccttcgtagt agaccggccg gcaggccgga agcaggacgg tggtcagtcc
      721 ctcggcgacc ggatgcgatt cgtcgaagtc ctggacgagc gcctcgaagg agagggaagc
      781 gccgcggaat gggtcctgga cctgctcgac gacgatctcg tcacccacgc ggatctggta
      841 ggccgagagg atgccgttga agtcgggtgc cgtgagcttc ttctcgcgtg ccacgtcggt
      901 gccggtaccc acatcgatgc agatcacggc gtggccgttg ttggacaggt agtctttgat
      961 cgcttgctct tcctcgggcg cgagcgccgt cttggctccc gcgatcacca gcgcggcgca
     1021 gtcatccggg accttgacga ctggggagct atccttcttg gcctcgtcac cggagccgct
     1081 ggccttgccg ccatcctccg tcttcgtctc cccggtgcgt gtcgaggcgc ccgaggtgag
     1141 ctcttcgagt gtggtggtgc tccgggggat ctggctgatc aggttcaggg tcttgatctc
     1201 atactgctgg tcggtgagca cagtcttgac gtcggaccac gccgcgcggt cccccatgcc
     1261 tccctgtggg tcaaccgggt actccccatg gccggtgagg aagtagacct tgggtttctc
     1321 gccggtcgtc accggccatg gggagtaccc ggttgaccca cagggaggca tgggggaccg
     1381 ggtactcccc atg
//
LOCUS       DAOSPL010001427         1392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_94472_length_1392_cov_3.460733, whole genome shotgun sequence.
ACCESSION   DAOSPL010001427 DAOSPL010000000
VERSION     DAOSPL010001427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94472_length_1392_cov_3.460733"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PLQ54_20355"
     CDS             <1..221
                     /locus_tag="PLQ54_20355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HOS95674.1"
                     /translation="EALGFQPRWSLEQGITQVIEAMRNGRVRDYRDARYSNYKFLSEE
                     GASLLGHQNGWADKLINDESVAVEAEMN"
     gene            326..844
                     /locus_tag="PLQ54_20360"
     CDS             326..844
                     /locus_tag="PLQ54_20360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95675.1"
                     /translation="MTSAGQSSALLIQRQAVEAVAYPQFAQLAWDSRDVMTADGLPLI
                     GLRTFESLEVRASGRAPARGLLRIAVSSGGSRGPDVVRLALQAGEAIPDANLFAVAGT
                     IDDRGVTGEEYARRALRQSAFGGAPTQYPEFAFCLWLQWVPAAGSSADPLALDWIALT
                     GFARPTLEVYGD"
     gene            858..>1392
                     /locus_tag="PLQ54_20365"
     CDS             858..>1392
                     /locus_tag="PLQ54_20365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95676.1"
                     /translation="MSWLPSRCPARVPVGALSAALVLCAVGGRADDQPQCTHRAVPSD
                     DWTYSAMRCLLRSIPVEGLSPVWFTGDYTFSAREMADMVWRAYEAASRAGALEESQIR
                     RWLAALAGEFCWELESMGRPVAEALARLRDGWTEGAQLFGQAETGVAASDEGSPVYGR
                     AQAGLAAFPNSPTHIAIV"
BASE COUNT          188 a          445 c          525 g          234 t
ORIGIN      
        1 gggaggccct gggcttccaa ccgcgctgga gcctcgagca ggggatcacc caggtcatcg
       61 aggccatgcg caatggccgt gtccgcgact atcgcgacgc ccggtacagc aactacaagt
      121 tcctcagcga ggagggcgcc tcgctcttgg gacaccagaa cggctgggcc gacaagctca
      181 tcaacgacga gtcggtggcc gtcgaggcgg agatgaactg aacgcctacc gccggagtcc
      241 cacgccgcgg cagggtccgc ggatcgccag gctttgcctg gctgcctctg cgttatcgat
      301 gggtgttgct ctggcgggct gcggggtgac ctctgcgggg cagagctcgg cgctgctcat
      361 ccagaggcag gccgttgagg ccgtcgccta tccccagttc gcgcaactcg cgtgggactc
      421 cagggacgtg atgacggccg acgggttgcc gctcattggc ctgcggacct tcgagagcct
      481 ggaggtgcgg gcgtcgggtc gcgcgccggc gagggggttg ctccgcatcg cggtctcgag
      541 cggcggcagc cgagggccgg atgtggtgcg cctggccttg caggcgggcg aagccattcc
      601 cgacgcgaac ctgttcgcgg tggccggcac catcgatgac cgcggggtga ccggtgaaga
      661 gtacgcgcgg cgggcgcttc gccagtcggc ctttgggggg gcaccgacgc agtaccccga
      721 gtttgccttc tgcctgtggc tgcagtgggt gccggccgcc gggtcgagtg ccgatccgct
      781 tgcgctcgac tggatcgcgc tgaccggctt cgcgcggcca accttggaag tgtacggaga
      841 ctgagggttc cttggccatg tcctggctgc catcccgatg cccggcccga gtgccggtgg
      901 gtgcgctctc ggcggcgctg gtcctgtgtg cagtgggggg gagggcagac gatcagcccc
      961 agtgcacgca ccgagcggtg ccgtcggacg actggaccta ctccgccatg cgctgcctcc
     1021 tccggagcat ccccgtcgag gggctgtcgc ccgtctggtt caccggcgac tacaccttct
     1081 cggcccgcga gatggccgat atggtctgga gggcctatga ggctgcgtcg cgggcgggcg
     1141 ctcttgagga atcgcagatt cggcggtggc tggcggcact cgccggcgag ttctgctggg
     1201 aactcgagtc gatgggcagg ccggtggccg aggcgttggc tcgcctgcgc gacggctgga
     1261 ccgagggggc ccagctgttt ggccaggccg agaccggcgt ggccgcatcg gacgagggtt
     1321 ccccggtgta tggaagggcc caggcagggc tcgcggcgtt cccgaactca cccacgcaca
     1381 tcgccattgt gg
//
LOCUS       DAOSPL010001428         1392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_94491_length_1392_cov_2.878833, whole genome shotgun sequence.
ACCESSION   DAOSPL010001428 DAOSPL010000000
VERSION     DAOSPL010001428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94491_length_1392_cov_2.878833"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PLQ54_20370"
     CDS             complement(<1..390)
                     /locus_tag="PLQ54_20370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95677.1"
                     /translation="MGELGKRLEELAGADPLSDEVTATRCKMAWCRRAADRYERPLPG
                     VPIETHIVRLGDIAFVTAPFEYYLDFGDRIKGRSPAVQTFVVQLTGDSPYLATERAAA
                     GLSYGAVPASCLVAPAGGQVLVDEAIAT"
     gene            complement(412..>1392)
                     /locus_tag="PLQ54_20375"
     CDS             complement(412..>1392)
                     /locus_tag="PLQ54_20375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95678.1"
                     /translation="DITPNGPVAIRGQFNLRIATRIEDPLTLTALALEAGDDHVILVS
                     IDSCTADQQVIDDSRAALAQRLPDVEAAKLIVCGTHTHTAPFASAEVGLQREEDYLAA
                     LRERYPDYVTTAEYSAQVTEALVDAVCDAWNARTEGHIGFGYAHAVVGENRRVRYFDN
                     RAQMYGSTSTADFSHIEGHVDHSVNLLCTYDSAQHLTGVLMDIACPAQASESGQDYVS
                     ADFWHDVREELRRRHGAGLFVLPQCSAAGDQSPHRLIAARAEDRMLRLKYGGRVDRPL
                     NTTLRRDIAQRLADAFDGAEPVLRRDLRDQAVLKHEYRRLSLPHWDVTDT"
BASE COUNT          197 a          469 c          490 g          236 t
ORIGIN      
        1 cgtcgcgatg gcttcgtcca ccagcacctg cccgccggcc ggcgcgacca ggcagctcgc
       61 cgggaccgca ccatagctca gtcccgccgc agcgcgctcg gtagccaggt atgggctgtc
      121 gcctgtgagt tggacgacga acgtctgcac cgcaggagaa cggcctttga ttcggtcgcc
      181 gaagtcgagg tagtactcga agggcgccgt gacgaaggcg atgtcgccca gccggacgat
      241 gtgtgtctcg atgggcacgc cgggcagggg gcgctcatag cgatcggccg cgcggcggca
      301 ccacgccatc ttgcaccgtg tggccgtgac ctcgtcgctc agcgggtccg cgccggcgag
      361 ctcctcgagc cgcttcccga gctctcccat ctcctcctgg agcgcggcgt actacgtgtc
      421 cgtcacgtcc cagtggggca gactgagccg gcgatactcg tgcttgagaa cggcctggtc
      481 gcgtaggtcc cggcgtagca ccggctccgc gccgtcgaac gcatcggcga gccgctgggc
      541 gatgtcgcgg cggagcgtcg tgttcagcgg tctgtccacg cgcccgccgt acttcagcct
      601 cagcatgcgg tcctcggccc tcgcggcgat caggcggtgc ggactctgat ctccagcggc
      661 cgagcactgc ggtaggacga acaggcccgc gccatggcgt cggcgcagct cctcgcggac
      721 atcatgccag aagtcggccg agacgtagtc ctgcccgctc tcgctggcct gggccgggca
      781 ggcgatgtcc atgagcacgc ccgtcagatg ctgggccgag tcgtacgtgc acagcaggtt
      841 cacgctgtgg tcgacatggc cctcgatgtg cgagaagtcg gccgtgctcg tgctgccata
      901 catttgggcg cggttgtcga agtatcgcac gcgacggttc tcaccgacga cggcgtgcgc
      961 gtagccgaag ccgatgtggc cctcggtccg tgcgttccag gcgtcgcaca ccgcgtcgac
     1021 gagtgcctcg gtgacctgag cgctgtactc ggccgtggtc acatagtccg ggtagcgttc
     1081 gcgcagggcc gcgaggtagt cctcttcacg ctgcagcccc acctcggcgc tggcgaaggg
     1141 ggcggtgtgc gtgtgggtcc cgcacacaat gagcttcgcg gcctccacgt ccgggaggcg
     1201 ctgggccagc gcggcccgcg agtcgtcgat cacctgctgg tcggcagtgc acgagtcgat
     1261 ggacaccagg atcacatggt cgtcgccggc ttccagcgcc agtgcggtca gcgtgagggg
     1321 gtcctcgatg cgcgtggcga tgcgcaggtt gaactgcccg cgaatggcca ccgggccatt
     1381 cggcgtgatg tc
//
LOCUS       DAOSPL010001429         1391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_94596_length_1391_cov_3.023204, whole genome shotgun sequence.
ACCESSION   DAOSPL010001429 DAOSPL010000000
VERSION     DAOSPL010001429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94596_length_1391_cov_3.023204"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..510)
                     /locus_tag="PLQ54_20380"
     CDS             complement(100..510)
                     /locus_tag="PLQ54_20380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95679.1"
                     /translation="MPPPLSKLIPMAVGAALILLLLSLAAWRRLSDAGRPDLGARAAR
                     NLLGTGLAVGAAAYVGAAAIGFDLLEYAARVQNGTAPWPPLGLAAASIALGVVLVARF
                     LSIMQAISNPAVAPAPGPAPGDDNGLGTADSVPR"
     gene            complement(593..>1391)
                     /locus_tag="PLQ54_20385"
     CDS             complement(593..>1391)
                     /locus_tag="PLQ54_20385"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOS95680.1"
                     /translation="PFLLTFGDIMTPPENYAALMAAYEATPGSAFLCVNRVDDVSSGG
                     AVFVEEGRVVDMIEKPPAGTVTTQFINSGIFILRPEVFPAIDDLTPSARGEYELTTAL
                     VRTIREGIPTHAHELQGYWSNVGDPDEVMWLTRLLLQRLAATRSKLERRLHRDDRFGG
                     PVFVGRNVDIHPDAQITGPVVIGDNCQIGNAHVGFYTTLGERCRVADGARVVSSAVLP
                     GTTIGENSHVGHALLGSGVRVRRNARLLGTCDQTTVVGDNGVIAAAS"
BASE COUNT          239 a          472 c          468 g          212 t
ORIGIN      
        1 ggggtcgccg gtctgcccga cggccgcctg gccgagcgag gccaccatca ggagaacgat
       61 cgcaggtcgc accatgggtc acaccgcccg atcgggggtc tagcgaggga ccgaatccgc
      121 ggttcccagg ccgttgtcgt cgcccggcgc cggtccggga gcaggcgcca cggcagggtt
      181 agagattgct tgcatgatcg acaggaaccg ggcgaccagc acgaccccga gggcgatgct
      241 cgccgccgcc aagccgaggg gcggccatgg cgcagtcccg ttctggacgc gagccgcgta
      301 ctccaggagg tcgaatccaa tggcggccgc accgacgtat gccgcggcac ccaccgccag
      361 tccggtgccc agcaggttcc gcgctgctcg agcgccaagg tcgggccgtc ccgcgtccga
      421 aagacgacgc caggcggcca ggctcagtag gagcaggatc agtgccgcgc cgacggccat
      481 cggaatgagt ttgctcagcg gcggaggcat gggtcgagta taccatgtcc cagccccgtg
      541 ctcggcgtgt ggcatcggcc ggtccgacgg agtgcccggc tcaactggag gtctagctgg
      601 ccgccgcgat gaccccgtta tcccccacca cggtcgtctg atcgcacgtg cccagcagcc
      661 gcgcattccg gcgaacgcgc actccgctac ccagcagcgc atgccccacg tggctgttct
      721 cgccgatggt cgtgccgggc aggaccgcgc tggagacgac tcgagccccg tcggcgacgc
      781 ggcacctctc gcccagtgtc gtgtagaacc ccacatgggc attgccaatc tggcagttgt
      841 cgccgatcac cacggggcct gtgatctgcg cgtccggatg gatgtcgaca tttcgaccca
      901 cgaacaccgg gccgccgaaa cggtcgtcgc ggtgtaaccg gcgctccaac ttgctccggg
      961 tagccgccag acgctggagc agaaggcgcg tgagccacat cacttcgtcg gggtccccca
     1021 cgttggacca gtacccctgg agctcgtgag catgggtcgg gatgccctcg cggatcgtcc
     1081 ggaccagcgc cgtggtcagc tcgtactcac cgcgagccga gggagtcagg tcgtcaatgg
     1141 cggggaatac ctccgggcgt aggatgaaga tgcctgagtt gataaactga gtcgtcaccg
     1201 ttccggccgg tggcttctcg atcatgtcga ccacccggcc ttcctccacg aagaccgcgc
     1261 ccccgctcga gacatcgtcg acacgattga cacacaggaa agccgagccc ggagtggcct
     1321 cgtatgcggc cataagggcg gcgtagttct cgggcggtgt catgatgtcg ccaaaggtga
     1381 gcaggaatgg c
//
LOCUS       DAOSPL010001430         1391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_94598_length_1391_cov_2.929641, whole genome shotgun sequence.
ACCESSION   DAOSPL010001430 DAOSPL010000000
VERSION     DAOSPL010001430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94598_length_1391_cov_2.929641"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1391)
                     /locus_tag="PLQ54_20390"
     CDS             complement(<1..>1391)
                     /locus_tag="PLQ54_20390"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOS95681.1"
                     /translation="RCECANCRALEEAEGSPSGPLLHFVNQVAEAIEPEFPQVAIDTL
                     AYQYTRKPPLHVRPRGNVIVRLCSIECSFADPLGTGDTNRSFAEDIEGWSKVCNRLYI
                     WDYTTNFAHYLLPHPNLRVLGPNVRFFVRNGVRGIFEQGAYTSAGAEFAELRAWVLAK
                     LLWDPEQDDQALIDEFLAGYYGQAAGPIGAYIKLMHDSCEVSDHYLSCFSSAQAPFLN
                     LEVLTEAKRLFAEAERLVAANAELLNRVRMAEVPVDYVFAIRWGELQQSAKLQGLDWP
                     IGESRADFSRRMIELLRANGATMHSEGSRLEALEQKLYGMPRQEAGPPPGCEDLPIGD
                     YLDLQDDGFSLYREGELSALAADPSASDGVAARMPATHIEWAVQQALGVPTDGTMDRW
                     TVCVVIRGDVRGQEGPAFSFGLWDSANGQPVASHAVGCADLADSSYQVYEIATISLKP
                     GLLLWVAPANNEA"
BASE COUNT          236 a          450 c          452 g          253 t
ORIGIN      
        1 cgcctcgttg ttggcgggcg ccacccacag caacaggccc ggcttcaggc tgatcgtggc
       61 gatctcgtat acttgatagc tcgagtcggc caggtcggca caccctaccg cgtggctcgc
      121 cacgggctga ccgtttgcgg agtcccagag gccgaagctg aaggctggcc cttcctgacc
      181 tcggacatca cctctgatga cgacacagac cgtccaccgg tccatggtcc catcggtggg
      241 cactcccagg gcttgttgaa cagcccactc gatatgagtc gcgggcatcc gtgccgccac
      301 gccgtcggaa gccgaggggt cggccgccag tgccgatagc tctccctcgc gatagagaga
      361 gaacccgtca tcctgcagat ccaggtagtc cccgatgggc aggtcttcgc acccgggggg
      421 cggtccagct tcctgcctcg gcatgccgta aagcttctgc tccagggctt cgaggcggct
      481 gccttcgctg tgcatggtgg cgccattggc cctgagcagt tcgatcatgc ggcgcgagaa
      541 atccgcgcgg ctctcgccga tgggccagtc gagtccctgc agcttggcgc tttgctgaag
      601 ctcgccccag cggatggcga agacatagtc gacgggcacc tcggccattc gcacgcgatt
      661 gagcagctcg gcgttggcgg ccaccagccg ttcagcctcg gcgaagagcc gcttggcctc
      721 ggtcagcacc tcgaggttca gaaacggcgc ctgggctgac gagaagcagc tcaggtagtg
      781 gtccgagacc tcacatgagt catgcatcag cttgatgtag gcgccgatgg gccccgccgc
      841 ctgaccatag taccccgcca ggaactcgtc gatcagggcc tgatcgtcct gctccgggtc
      901 ccagagcagt ttcgcaagta cccaggcccg cagttcggcg aactcggccc ctgccgatgt
      961 gtaggccccc tgttcgaaga tgcccctgac gccgtttcga acgaagaagc gcacgttggg
     1021 tccgaggacc cggaggttcg gatgcggcag cagatagtga gcgaagttgg tcgtgtaatc
     1081 ccagatgtag aggcggttgc agaccttcga ccacccctcg atgtcctcgg caaatgaccg
     1141 gttggtgtcg ccggtcccca gcgggtcggc gaaggagcac tcgatgctgc acaggcggac
     1201 gatgacgttc ccccgcggac gcacatggag gggtggtttg cgcgtgtact ggtacgctaa
     1261 cgtgtcgatg gcaacttggg ggaactccgg ctcgatggcc tcggccacct ggttgacgaa
     1321 gtgcagcagc gggcctgatg gtgatccctc ggcctcctcg agcgccctgc agttggcgca
     1381 ctcgcagcgg c
//
LOCUS       DAOSPL010001431         1389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_94774_length_1389_cov_3.754123, whole genome shotgun sequence.
ACCESSION   DAOSPL010001431 DAOSPL010000000
VERSION     DAOSPL010001431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94774_length_1389_cov_3.754123"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1389
                     /locus_tag="PLQ54_20395"
     CDS             <1..>1389
                     /locus_tag="PLQ54_20395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95682.1"
                     /translation="NRAPYYGFRDFDAAVKHPAFIHRAGLVSVVELGEETTNGRRWRR
                     QLHIAPCARRAQADALAELADEGLVIDVTPRTASLGPGPIRVSAPPEARVPAEVLADA
                     LRARGVEAAVAGPPAAAQVALRIATQADAPGISGDGFAIELRGGGVTITALTPFGLTQ
                     GAQRLAEYLADATGGVRAPLIAENPASNLRAGGFGGGEHEVDFPYGTDDEWEHALGGM
                     IASGMNTMADLSMWGNWKMPVTYRYMPELQSNSPDAIDPASGARFSEIPAHRERGLRL
                     LDYLHRRGVKVWQWIPVGCVPSTFATAHPEAMSKTNPGVPCFTDPSYACYLEAYAREL
                     LETYPIDGIVMIRDDNGGICQCDRCQAYVDASRTRNAMWEQHILLYGQVRSLGFAGDI
                     AVYPYMDPYQPELDALLPADLYIVGHGSGAGMLSRSLDTLGPMGDTWLDNLFASFRPP
                     SIPRMKRLLADRG"
BASE COUNT          210 a          486 c          467 g          226 t
ORIGIN      
        1 aaccgggcgc cgtactatgg gttcagggac ttcgatgccg ccgtgaagca cccggcgttc
       61 atccaccgtg ctggcctcgt gtctgtggtc gagctgggcg aggagacgac caacgggcgc
      121 cggtggcgcc gtcagctcca tattgcccct tgtgcccgcc gggcacaggc tgacgccctg
      181 gccgagctgg ccgatgaggg actcgtgatc gacgttacgc cccgcacggc atccttgggg
      241 ccggggccca tccgtgtatc cgcgccgccg gaggcgcggg tccctgccga ggtactggcc
      301 gacgcgctgc gcgctcgtgg tgtcgaggcc gccgtcgccg gtccgccggc agcggcccag
      361 gttgcgcttc gcatcgccac tcaggccgat gcgcctggta tcagcggcga cgggttcgcc
      421 atcgagctcc gcggcggcgg cgtcactatc accgcgctga cgcccttcgg cctcactcag
      481 ggcgcgcagc ggctcgcgga gtacctggcc gacgccacag gtggggtgcg cgcgccgctc
      541 atcgcggaga accccgccag caacctccgg gcaggcggct tcggtggcgg cgagcacgag
      601 gtcgacttcc cctacggcac cgacgacgaa tgggagcacg ccctcggcgg catgatcgcc
      661 agcggcatga acaccatggc cgacctgagc atgtggggca actggaagat gccggtgacg
      721 taccggtaca tgccggagct gcaatccaac tcgccggacg ccatcgaccc ggcgtcgggg
      781 gcccggttct ctgagatccc cgcgcatcgc gaacgcggcc tcaggctgct ggactacctg
      841 caccggcgtg gtgtgaaggt gtggcagtgg atacccgtcg ggtgcgtgcc gtccaccttt
      901 gcgaccgctc atcccgaggc catgtcgaag acgaacccgg gggtgccctg cttcaccgat
      961 ccgagctatg cctgctatct cgaggcctac gcgcgggagc tgctcgagac ataccccatc
     1021 gacggcatcg tcatgatccg cgatgacaat ggcgggatct gccagtgcga tcggtgccag
     1081 gcttatgtgg acgcgtcccg cacgcggaac gccatgtggg agcagcacat cctgctctac
     1141 ggccaagtgc gatctctcgg cttcgcgggc gacatcgccg tctatccgta tatggatcca
     1201 taccagcccg agctcgacgc gttgctcccg gccgacctgt acatcgtcgg gcatggcagt
     1261 ggggcgggta tgctcagccg gagtctcgac accctcggtc cgatgggcga cacgtggctc
     1321 gacaacctgt tcgccagctt ccggccgcca tcgatcccgc gcatgaaacg cctgctcgcc
     1381 gaccgcggc
//
LOCUS       DAOSPL010001432         1389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_94813_length_1389_cov_2.260120, whole genome shotgun sequence.
ACCESSION   DAOSPL010001432 DAOSPL010000000
VERSION     DAOSPL010001432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94813_length_1389_cov_2.260120"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PLQ54_20400"
     CDS             complement(<1..1101)
                     /locus_tag="PLQ54_20400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95683.1"
                     /translation="MPRPVTVAAALLALTGLALAAPETIIDTDFGAPGRAFEIIDAAK
                     GNRIVGSLPEGWSDNSGWKSDVVAEYRTTTEDGRTFLRITQTSGDGLQFMHDLPGIGK
                     GGGYYRLTVTARGRTGCSLALRFNGPPYTSPWTASPATGDEWRDFSYDFRLPEQPQPL
                     GLYCYLPANGSLDLQSLKLVRLSDEDLIAEVKAKYPEGGQGNLLRTTRFPLGLQSGWS
                     IAREYSDGDQVQVHTDAQVIGPSGCPALRIDAPLGITIYGEPFLVPWSFEPHVASVYV
                     RGNWDGRLVAIGGQVEYCGECPIPIDTTEWQRVQVAFKPILLARSHSLMFEGRGTLWI
                     DGLQVERGAEASPYAPRRAAEVSLALPESDASA"
BASE COUNT          223 a          477 c          471 g          218 t
ORIGIN      
        1 ggccgaggca tccgactccg gcagcgccag cgagacctcg gcggcgcggc gtggggcgta
       61 gggactcgcc tcggcccccc gctcgacctg caggccgtcg atccagagcg tgcctctgcc
      121 ctcgaacatg agactatggc tccgtgcgag gagtatgggc ttgaaagcaa cctgcacgcg
      181 ctgccattcg gtcgtgtcga tcgggatcgg gcactcgccg cagtactcga cctggccgcc
      241 gatggcgacc agcctcccgt cccagttgcc ccgcacgtac acgctggcca cgtgcggctc
      301 gaagctccac ggaacgagga aaggctcgcc atagatggtg atgccgagcg gggcgtcgat
      361 ccgtagcgcg gggcagccac tcgggccgat gacctgcgca tcggtgtgga cctgcacctg
      421 gtcgccatcg gagtactcgc gagcgatgga ccatccgctc tgcagcccga gaggaaagcg
      481 cgtggtccgc agcaggttgc cctggccgcc ctccgggtac ttggccttca cctccgcgat
      541 caggtcctcg tcagagaggc gaacgagctt caggctctgc agatctaggc tgccgttcgc
      601 cgggaggtag caatacagac ctagcggttg tggctgttcg ggcaggcgga agtcgtacga
      661 gaagtcgcgc cactcatcac cggtggccgg ggaggccgtc cacggggacg tgtagggcgg
      721 gccgttgaaa cgcagggcca gactgcagcc cgtgcgcccg cgcgccgtga cggtgaggcg
      781 gtagtagccg ccccccttcc cgatgcccgg caggtcgtgc atgaactgaa ggccatcgcc
      841 agaggtctgc gtgatgcgca ggaaggtccg gccgtcctct gtcgtagtcc ggtactcggc
      901 caccacgtcg ctcttccacc cggagttgtc cgaccacccc tcgggcagag agccgacgat
      961 gcggttacct ttcgcggcat cgatgatctc gaacgccctg ccgggcgcgc cgaagtcagt
     1021 atcgatgatg gtctctgggg cagccagggc aaggccagtc agagccagta gcgccgctgc
     1081 cacagtcacg ggcctgggca tggccagaca cctcccacag gggatagaca gtgccgcagg
     1141 cccgttcggc atccgagcac ggcgtgcctc cagattgccc tggcggcggc ccccgcagcg
     1201 cccctcgcgt aggtgcgcgc cccgaggcgc gcgaatgcgc gggccatggc actctggcgc
     1261 tgcttgcccc tcgtgatgcc gctcctcagt ctgacggtcg gcccaagcca tgccgagctc
     1321 gctatcgaac tcgacgaccg tctggctgcc atcgaggcgg gagaccagcg ggtcgagttc
     1381 cgcctcaac
//
LOCUS       DAOSPL010001433         1388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_94888_length_1388_cov_2.855214, whole genome shotgun sequence.
ACCESSION   DAOSPL010001433 DAOSPL010000000
VERSION     DAOSPL010001433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94888_length_1388_cov_2.855214"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLQ54_20405"
     CDS             complement(<1..182)
                     /locus_tag="PLQ54_20405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95684.1"
                     /translation="MSARWGVLLVFVALGGPTLGQGEEAPVVHGPAWFDRGATYYSAF
                     EGGLDAPEVNRAEAVLV"
     gene            complement(179..>1388)
                     /locus_tag="PLQ54_20410"
     CDS             complement(179..>1388)
                     /locus_tag="PLQ54_20410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95685.1"
                     /translation="LALGDCGSVYPAGRDLYLWEMIARGNRERLSEADVQAVIDRGRP
                     YFEPYGEERVKTFEAHARYNLTSRYGTRMIFYYNRASYQLAEEFQTFQDEWCLSNYRT
                     VGEGNGIGEIKIVPSESYIDHALYWYDKSFDIGGNQGVYWDNWFFAGSYNTHETAAYT
                     RSDGTVVPSTGVWGLRELSKRTFQLMNERGMLPITMPHMTSTNILPLHSFATVQYDWE
                     WKYSEGDVQGRFPRDYILLVSNGELAGTWPVLLNDHGAQAEDPWTARTFAAVAMVHEL
                     DCPYPAWSEAGRQQLALFAPVDEILARPGVDAYRYWDERPQPMSTDDPDLPAIVYVVP
                     GEEAVCAVVSYADEDRAARVRIDTAALGMPGGCIVTDAETGAELAQSTGGLVLPLKRH
                     DVRVLRVVSR"
BASE COUNT          238 a          492 c          422 g          236 t
ORIGIN      
        1 acgagaacgg cctcggcacg gttcacctcc ggcgcgtcca gtcccccttc gaacgccgag
       61 tagtacgtcg cgccgcgatc gaaccacgcc ggtccgtgaa cgacgggcgc ctcctcgccc
      121 tgccccagcg tcggccctcc cagcgccacg aacacaagca gcactcccca acgggcgctc
      181 atcgcgacac cacccgcagc acgcgcacat cgtgccgctt gagcgggagc acaagccccc
      241 cggtgctctg tgccagttcg gcccccgtct ccgcatcggt cacgatgcag ccaccgggca
      301 ttccgagcgc tgcggtgtcg atgcgaactc gggccgcccg gtcctcgtcg gcgtagctga
      361 ccaccgcgca caccgcctct tcgccgggca cgacgtacac gatggccggc aagtccgggt
      421 cgtcggtcga catgggctgg ggccgttcat cccagtagcg gtaggcgtca acgcccggcc
      481 gagcgaggat ctcgtctacc ggcgcgaaca gcgccagttg ctgcctgccc gcctccgacc
      541 acgccgggta ggggcagtcg agttcgtgga ccatcgccac ggccgcgaac gtccgtgccg
      601 tccaggggtc ttccgcctgc gcaccgtggt cgttcagcag cacgggccac gtccccgcca
      661 gttcaccatt gctcaccaga aggatgtagt cccgagggaa acggccctgg acgtcaccct
      721 cggagtactt ccactcccag tcatactgga ccgtcgcgaa cgagtgcagg gggaggatgt
      781 tggttgaggt catgtgcggc atggtgatgg gcagcatgcc ccgctcgttc atgagctgga
      841 aggtccgttt cgagagttcc cgcaggcccc agacgccggt cgagggcacg actgttccat
      901 ccgacctcgt ataggcggcc gtctcgtggg tgttgtagga cccggcgaag aaccagttgt
      961 cccaatagac cccctggttg ccgccgatgt cgaacgactt gtcataccag tacagcgcat
     1021 ggtcgatgta cgactcgctg ggcacgatct tgatctcgcc gatgccgttc ccctcaccga
     1081 cggttcgata gttggaaagg caccactcgt cctggaacgt ctgaaactcc tcggcaagct
     1141 ggtagctcgc ccggttatag taaaagatca tcctggtgcc atagcgcgag gtcaggttat
     1201 agcgggcgtg ggcctcaaac gtcttcacgc gctcttcacc atagggctcg aagtagggcc
     1261 gtccgcggtc gatgacggcc tggacgtcgg cctcggacag ccgctcccgg ttccctcggg
     1321 cgatcatctc ccacaggtac aggtcccgcc cggccgggta caccgaccca cagtccccca
     1381 aagccagc
//
LOCUS       DAOSPL010001434         1385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_95213_length_1385_cov_3.051128, whole genome shotgun sequence.
ACCESSION   DAOSPL010001434 DAOSPL010000000
VERSION     DAOSPL010001434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95213_length_1385_cov_3.051128"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1385)
                     /locus_tag="PLQ54_20415"
     CDS             complement(<1..>1385)
                     /locus_tag="PLQ54_20415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95686.1"
                     /translation="LLLPGAWSAGIDSGGTTLELASTAEAPLALQANADGNDEDYPKM
                     NRIWDEPQDWSAYMRMRCRVRVLCDTPGVGQKQLAFVYYDAKTLRQDLPDHPMTQQVV
                     SHTVPVNQWVTINDWILDINRTAIRTLSIYLYELPPAAAHSYRWEFAELALEGVGELA
                     VAFDTETYADGAFRATPGPEAQAVAALPLELGLTADGSIAQVLVRGQRVGGATDQVSG
                     LLVRDVAVGGPPAPVGGAVRREGDAVAQSAELADLGLGVEATYRAEGDTIRVSGLIRD
                     LRGQDRAVTVTLAVPLEGWDWRWWDSVDGSRTAAPDDNKEYAYYETGAEYGVNGAHSK
                     YPIGAVTSPERAGLTLAVRMDQPVVHRIAYTPKLRLFHISADLGLVAGIAAKGRPLSE
                     APFEFLLYPHDPAWGFRSALQHYYTAFPDFFTGRVERQGGWYVWGDVSQTEGALEAGF
                     GFHWGPVGPDA"
BASE COUNT          211 a          507 c          457 g          210 t
ORIGIN      
        1 cggcgtccgg cccgaccggc ccccagtgga acccaaagcc agcctcgagc gcgccctcgg
       61 tctggctcac gtcgccccac acataccacc cgccctgccg ctccacgcgg cctgtgaaga
      121 agtccgggaa cgccgtgtag tagtgctgca gggccgagcg gaacccccag gccggatcgt
      181 gcgggtagag caggaactcg aatggcgcct cggacagcgg ccgtcccttg gccgcaatgc
      241 cagccaccag tccgagatcc gcgctgatgt ggaacagccg cagtttgggg gtgtaggcga
      301 tgcggtgcac cacgggctgg tccatccgaa cggccagggt caggccagcg cgctccggtg
      361 aggtcaccgc gccgattggg tatttcgaat gggcgccgtt gaccccatac tccgcccccg
      421 tctcgtagta ggcgtactcc ttgttgtcgt ccggtgccgc cgtgcgcgag ccgtccacac
      481 tgtcccacca tcgccagtcc cagccctcga gcggcacggc gagggtgacg gtgacggccc
      541 gatcctggcc gcgaaggtcg cggattagcc cggagacgcg gatcgtgtcg ccttcggccc
      601 ggtacgtggc ctcaacgccc aaccccaagt ccgccagctc tgctgactgc gccacggcat
      661 cgccttcgcg ccgaaccgcg ccaccgactg gcgccggggg cccgccgacg gccacatcgc
      721 gcaccagcaa gccactcacc tgatcggtgg ccccgcccac acgctggccg cggaccagca
      781 cctgagcgat cgatccatcg gcggtgaggc cgagctctag cggcaaggcc gccaccgcct
      841 gcgcctcggg cccgggcgtc gcccggaacg cgccgtcggc ataggtctcc gtgtcgaacg
      901 ccacggccag ctcgcccacg ccctcgagcg ccagctcggc aaactcccac cggtacgagt
      961 gcgccgcggc cggcggcagc tcatagaggt agatcgacag cgtgcggatg gcggtccggt
     1021 tgatgtcgag gatccagtcg ttgatcgtga cccactggtt caccggcacc gtgtgcgaga
     1081 ccacctgctg cgtcatgggg tggtcgggca ggtcctgccg cagcgtcttc gcgtcgtagt
     1141 agacgaacgc cagctgcttc tgacctacgc ctggcgtatc gcagaggacg cgcacgcggc
     1201 accgcatccg catgtaggcc gaccagtcct gcggctcgtc ccagatgcgg ttcatcttcg
     1261 ggtaatcttc gtcattgccg tcggcgttgg cttgcagggc gagcggcgcc tcggcggttg
     1321 aggccagctc cagcgtcgtg ccgccgctat cgatgccggc cgaccaggcg cccggcagca
     1381 gtagg
//
LOCUS       DAOSPL010001435         1385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_95219_length_1385_cov_2.923308, whole genome shotgun sequence.
ACCESSION   DAOSPL010001435 DAOSPL010000000
VERSION     DAOSPL010001435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95219_length_1385_cov_2.923308"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PLQ54_20420"
     CDS             <1..436
                     /locus_tag="PLQ54_20420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="in Escherichia coli this enzyme functions in
                     thiamine biosynthesis along with thiFSGI and IscS; with
                     ThiFSG catalyzes the formation of thiazole phosphate from
                     tyrosine, cysteine and 1-deoxy-D-xylulose-5-phosphate;
                     forms a complex with ThiG; contains an iron-sulfur center;
                     in Thermotoga this enzyme has an extra C-terminal domain;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOS95687.1"
                     /translation="AHPGCPEALMGVTQCSVGSLSYPGAYSGDGEPETNSTLRIGSPR
                     ALEQLVYRMAEFGFVPNLCASCAVAGRRPAGARSRCVNERCGPNSLAALKEYLLDHAS
                     PETQVLGDRLIQRELSRLPERLRSDTLDLLQEVEAGLRRQVL"
     gene            482..>1385
                     /locus_tag="PLQ54_20425"
     CDS             482..>1385
                     /locus_tag="PLQ54_20425"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOS95688.1"
                     /translation="MNREVPPSTGVVTTILDRCRRCYSCVRGCPAKAIRVQGGQAHVI
                     EERCIGCGTCVRVCRQKAKQVLSALPAARQLLSGTEPIVAIVAPSYPAAFEGVSAGQV
                     VAGLRQLGFARVYEVGFGADMVAREYAHLVKRRPAAALISTPCPALVAYVCKYAPSLV
                     PFLAPVVSPMAAMARAVRRSLMPGAPVVFVGPCIAKKAELRNAGDASSADLAMTFTEL
                     DELLRERDVDVNRLAPSVADEPLPGLGALFPITGGLLKAAMVRADLTDDGVIAVDGPR
                     RCIETLRAMEQGALGPCLVDALWCE"
BASE COUNT          210 a          454 c          486 g          235 t
ORIGIN      
        1 tgcgcatcct ggttgcccag aggctttgat gggcgtcacg cagtgtagtg tcggctcgct
       61 aagctatccg ggtgcctaca gtggagacgg agaacccgag acgaactcca cactgcggat
      121 cggtagcccg cgagcccttg agcagctcgt gtaccggatg gccgagtttg ggttcgtccc
      181 gaacctgtgc gccagctgtg cggtggcggg gcgccgaccg gccggggcgc ggagccggtg
      241 tgtgaatgaa cgctgcgggc ccaattcgct ggctgcactc aaggaatacc tgcttgacca
      301 tgcctcgccc gaaacccagg tcctgggcga ccgcctgatc cagcgggagc tttcccgcct
      361 ccccgagcgt ctacgttccg atacactgga cctgctgcag gaggtcgaag cagggctccg
      421 gagacaggtg ttgtagcgac gctgggccgc gggcgcggcc cgagtcagca ggtgaagacg
      481 cgtgaaccgc gaggtcccac ccagcacggg cgtcgtgacg accatcctcg atcggtgccg
      541 ccgatgctac agctgtgtcc gtggctgccc cgccaaggcc atccgcgtcc agggcggcca
      601 ggcccacgtc atcgaggagc gctgcatcgg ctgtggcaca tgcgtcaggg tctgccggca
      661 gaaggccaag caggtgctct ccgctctgcc tgcggcacgc caactgctct caggcacgga
      721 gccgatcgtg gccattgtcg ctccctcata ccccgccgcg ttcgagggcg tctcggctgg
      781 ccaggtcgtg gcgggactgc gccaactcgg tttcgccagg gtctacgaag tcgggtttgg
      841 cgcggacatg gtggcccgcg aatacgcgca cctcgtcaag cggcgcccgg cggcggccct
      901 gatcagcact ccgtgtccgg cactggtcgc ctatgtctgc aagtacgcgc cgagcctcgt
      961 accctttctc gcgccggtcg tgtcgccgat ggcagccatg gctcgggcag tgagacggtc
     1021 gctgatgccg ggagcaccgg tggtcttcgt ggggccctgc atcgccaaga aggcggaact
     1081 gcggaacgcc ggcgacgcgt ccagcgcgga tctggccatg acgttcaccg agctcgacga
     1141 gcttctgcgg gagcgggacg tggacgtgaa ccgcctggcg ccatcggtcg ccgacgaacc
     1201 cctgcccggc ctgggggccc tgttccccat caccggtggg ctactcaagg cggcgatggt
     1261 gcgtgctgac ctgacggatg acggcgtgat cgctgtcgac gggcccaggc gctgcatcga
     1321 aactctgcgc gccatggaac agggagcgct cgggccatgt ttggtcgatg cgctgtggtg
     1381 cgagg
//
LOCUS       DAOSPL010001436         1384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_95338_length_1384_cov_2.770504, whole genome shotgun sequence.
ACCESSION   DAOSPL010001436 DAOSPL010000000
VERSION     DAOSPL010001436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95338_length_1384_cov_2.770504"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PLQ54_20430"
     CDS             complement(<1..776)
                     /locus_tag="PLQ54_20430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95689.1"
                     /translation="MATNSTSGPTGSKARRLLLRVEAIGKTDRLKGVPYEELLWYEGR
                     RAGLPWPSVWWWLQGIGIVAGAADETPLAEQAAAVAVDQVRLALRTPQGMAQSRRSLD
                     EGPDLALSLRNAMIWANRALRESAQGQADRRATVGASVACAAMRGNKTAVAWIGDCRC
                     YHVRGQTCSLIDDPVGASTSPGVPHLDPSPVTPATGTPDSGLGAADDIQPCVRELDVE
                     VGDVLVLLSPGVRHVLDGPDDIARYVAEADGAEALYDLKA"
     gene            932..>1384
                     /locus_tag="PLQ54_20435"
     CDS             932..>1384
                     /locus_tag="PLQ54_20435"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOS95690.1"
                     /translation="MVARVQAVPLQNEDVSFRLDDVERVRYNSGLAMLRPYLFPVIGP
                     AGRMVTRMGHPHDPYGHRHHYSVWIAHQKVNGCDLWADHGLTGRQAHSKIVALEDGDA
                     SAALAVEVVWRGPQNQELLREVRTYRVTCLPAGELLIDMGLELAATQGE"
BASE COUNT          198 a          457 c          508 g          221 t
ORIGIN      
        1 tcggccttca ggtcgtagag ggcctccgcg ccgtccgctt cggccacgta gcgcgcgatg
       61 tcgtccgggc cgtcgagcac gtggcggaca ccgggggaga ggagcaccag gacgtcgccc
      121 acctcgacat cgagctcacg gacacagggc tgaatgtcgt ccgctgcgcc gagcccgctg
      181 tcgggcgttc cggtggccgg tgtcacggga gaggggtcga ggtggggcac gcccgggctg
      241 gtgctggccc ccaccgggtc gtcgatcagg gagcaggtct ggccgcggac gtgatagcag
      301 cggcagtcac cgatccaggc cacggccgtc ttgttacctc gcatggcggc acacgcgacc
      361 gaggcgccca cggtcgcgcg gcggtcggcc tggccttggg ctgactcgcg cagggctcgg
      421 tttgcccaga tcatggcgtt tcggagcgac agggccagat cgggcccctc gtcgagactg
      481 cggcgcgatt gggccatgcc ctggggtgtc cgtagcgcca gtcgaacctg gtcgacagcg
      541 acggcggcgg cctgttcggc gaggggggtc tcgtcggccg ctccggcgac gatgccgatg
      601 ccctggagcc accaccagac gctgggccag ggcaagcccg cccgtcggcc ctcataccac
      661 agtagctcct cgtagggcac tcccttgagc cggtctgtct tcccaatggc ctcgacgcgc
      721 aggagcagcc tacgcgcttt cgacccggtc ggtccggagg tgctgttcgt cgccatctcc
      781 ctcatcacac agccggtggg attgcgccgc cgcggattcg cgcggccatg ttcagccgag
      841 gggcgccgga gtccttggtg agcggcggca gcgccaggtg taggacacga gctgtggcgc
      901 atcgaactgg ccctccgccg aggtgagacc catggtggct cgagttcagg cagtgcccct
      961 gcagaacgaa gacgtgagct tccgcctcga tgatgtggag cgcgtgcgct acaacagtgg
     1021 gttggcgatg ctccggccct acctgttccc ggtcatcggt ccggcgggcc gcatggtcac
     1081 gcgtatgggg catccgcacg acccgtatgg gcaccggcac cactactcgg tgtggatcgc
     1141 gcatcagaag gtcaacggct gcgatctctg ggcggaccac gggctcacgg gccggcaggc
     1201 gcactcgaag attgtcgcgc tcgaggatgg cgatgcctcg gccgctctgg ccgtcgaggt
     1261 cgtctggcgc gggccgcaga accaggagtt gctgcgcgag gtacggacct accgggtcac
     1321 gtgtctaccg gccggcgagc tgctgatcga catggggctg gagctggcgg ccacgcaggg
     1381 cgag
//
LOCUS       DAOSPL010001437         1381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_95624_length_1381_cov_2.574661, whole genome shotgun sequence.
ACCESSION   DAOSPL010001437 DAOSPL010000000
VERSION     DAOSPL010001437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95624_length_1381_cov_2.574661"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1381)
                     /locus_tag="PLQ54_20440"
     CDS             complement(<1..>1381)
                     /locus_tag="PLQ54_20440"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOS95691.1"
                     /translation="AADAPGRRAVLLITDGRHEGGLAELDDVLLLARQGAIPISCIGI
                     GVDVDGAACRRIAAATGGDARFAFTADDLPARVGELAHTYGSAHTLAYRSPRPDTDGA
                     TRALAWTAADGTEARLLYRAPLPPGQCGKLLVRALGADGSALPVAYRVMGEQGGDPVD
                     AGVAGRQPVLLPPGRFLVEAQGPFGPPEAVEVAAGRSSALVFRLESRLMVHAPPSTRG
                     EPARVAVRDAGTGELVNIGFAETAMDLPPGEYAVDVGEHPRSSHDRVRLRPGRTEALW
                     VANTGVALVRAANAEGKPLAGAVFLQDPTTRQVQSIGRLCEPLAVPAATYLLDIEAPH
                     TGGLPPLVVRPGAVSEASIGALARVTIEYEGAAGMPVPVAFVRALATDRTFALGDVTE
                     FDIGPGEYELALETIPRIEQALTVSAGEWLKFEFRRFGSLTVGGPEGRRFSLRTRPDG
                     DRPGAWLGVF"
BASE COUNT          217 a          497 c          487 g          180 t
ORIGIN      
        1 gaacacgccg agccaggcac cgggccggtc gccatcgggt ctcgtcctta gggagaagcg
       61 gcgaccctca ggcccgccca cggtgagact cccgaaacga cgaaactcga acttgagcca
      121 ctctccggcg cttacggtca atgcctgctc gatccgcggg atggtctcca gcgcgagttc
      181 gtactcgccc ggtccgatat cgaactcggt gacatcaccc agggcaaagg tccggtcagt
      241 cgccagcgcc cggacgaatg ccaccggcac cggcatcccc gcagccccct catactcgat
      301 ggtgacccgg gccagtgccc cgatggaggc ttcactcaca gccccaggcc ggaccaccag
      361 gggagggaga ccgcccgtgt gaggtgcctc gatgtccagg aggtaggtgg ccgcggggac
      421 ggccaagggc tcacacaggc gcccgatgga ctgcacctgc cgcgtggtcg ggtcctgcag
      481 aaacacggcg ccggccagcg gcttgccttc ggcgttggcg gcgcggacca gtgcgacgcc
      541 ggtgttggcc acccacaggg cctcggtgcg tccaggccgc aggcggacgc gatcgtggct
      601 gctgcgcggg tgctcgccca catccacggc gtactcgccc ggcggcagat ccatggccgt
      661 ctcggcgaac ccgatgttca ccagttcgcc ggtgccggca tcgcggacgg cgacccgtgc
      721 cggctcgccg cgtgtgctcg gcggggcgtg gaccatcagt cgactctcca gccggaagac
      781 cagggcgctg gaccggcccg cggcaacctc gacggcttcg ggtgggccga aagggccctg
      841 ggcctcgacc aggaaacgcc cgggcggcag aaggacgggc tgtcggccgg ccaccccggc
      901 gtcgacgggg tcgccgccct gctcgcccat cacgcggtag gccacgggca aggcgctccc
      961 atcagcaccg agggcgcgca ccaacagctt tccgcactgc ccgggaggaa gcggcgcccg
     1021 gtagagaagt cgtgcctcgg tgccgtcggc cgccgtccat gcgagggccc gggtggcgcc
     1081 atcggtatca ggacgaggtg acctgtacgc gagtgtatga gccgagccgt aggtgtgtgc
     1141 cagctcgccc acccgggcgg gcaggtcatc ggccgtgaag gcgaagcggg catcgccgcc
     1201 cgtggccgcg gcgatcctcc ggcatgccgc gccgtcgaca tccacaccga tgccgatgca
     1261 agagatcggt atggccccct gccgggccag cagcagcacg tcgtcgagtt cggccagacc
     1321 tccctcgtgc cggccatcgg taatgagcag gacggcgcgg cgccccggcg catcagcggc
     1381 g
//
LOCUS       DAOSPL010001438         1381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_95637_length_1381_cov_2.388386, whole genome shotgun sequence.
ACCESSION   DAOSPL010001438 DAOSPL010000000
VERSION     DAOSPL010001438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95637_length_1381_cov_2.388386"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..>1381)
                     /locus_tag="PLQ54_20445"
     CDS             complement(23..>1381)
                     /locus_tag="PLQ54_20445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3395 domain-containing protein"
                     /protein_id="HOS95692.1"
                     /translation="GPMGSVFVVFRAPASGGATFTDIERDGVSALALQGGPKHTLTID
                     RAIYGVLASDVPDCVDLTEKVQAALRDGRVSVPATNAVAGVDPAPNVVKQLRVDYTIA
                     NQPLVGVADENETLSLPRPTDGAGDIAIVRALYGLVPPGPYRPIGGQTVDVTEAVAAL
                     VRDGTLVVTAGNELGGDPANLVRKQMRVDYTLDGKRHSRTIQEGATLQLPEGTASPGF
                     PAAELECADDGSALLVAWLPGTYELTSSSGRKRSVEVTHVPDPAEVAGPWDLSFPPGL
                     GAPARVALDQLISWTDHPDEGVRYFSGTATYVTHVDIAAHALADGQRVALDLGDVREI
                     AEVRLNGTDLGILWKPPFRVDVTSAAKPGRNRLEVRVTNLWPNRLIGDEQLPPDAEYS
                     PGGPIRAWPEWLVKGTPRPATGRVTFTTWQHWTKDSPLLESGLLGPVTLHVARVVPVG
                     KP"
BASE COUNT          214 a          495 c          470 g          202 t
ORIGIN      
        1 gccgccggcg cgctgacccc gcctacggct tccccaccgg cacgacgcgc gcaacgtgga
       61 gcgtgaccgg tccgagcagg ccggactcca gcagcggcga atccttcgtc cagtgctgcc
      121 atgtggtgaa agtgacccgt ccggtcgcgg gccgcggcgt gcccttcacg agccattccg
      181 gccacgctct gatggggcca ccagggctgt actcggcatc gggcgggagc tgctcgtccc
      241 cgatcagccg attgggccag aggttggtga cccgcacctc cagcctgtta cggccgggct
      301 tcgcggcgga agtgacgtcg acgcggaacg gcggcttcca caggatgccc aggtcggtgc
      361 cgttgagcct cacttcagct atctcgcgca cgtctccgag gtcgagcgcg acccgctgcc
      421 cgtcagctag cgcgtgggcc gcaatgtcca catgggtcac gtaggtcgca gtaccggaga
      481 agtaacgcac gccctcatcc gggtggtcgg tccacgagat gagctggtcg agcgccaccc
      541 gcgccggcgc gcccaggccg ggcgggaatg acaggtccca cggcccggcg acctctgcgg
      601 ggtccggcac atgggtcacc tcgaccgacc gcttccgccc cgaggacgat gtgagctcgt
      661 aagtccccgg cagccacgcc accagcaagg ccgagccatc gtccgcacac tcgagctccg
      721 ctgcggggaa gcccggactc gccgtaccct ccggtagctg gagcgtggca ccctcctgga
      781 tggtccgcga gtggcgcttc ccgtcaagcg tgtagtccac acgcatctgc ttgcggacca
      841 gattggccgg gtctccgccc aactcgttgc ccgccgtgac cacgagcgtg ccgtcgcgga
      901 ccagagccgc cacggcctcc gtcacatcca ccgtctggcc gccgatgggc cggtaggggc
      961 ccggggggac caggccgtag agagcccgca cgatggcgat gtcgcccgca ccgtccgtgg
     1021 ggcgcgggag gctcagcgtc tcgttctcat cagcgacgcc caccagcggc tggttggcga
     1081 tggtgtagtc gacccgaagc tgcttcacaa cattgggagc gggatcgacg ccggcgacgg
     1141 cgttggtcgc cggcacggac acgcgcccgt cgcgcagtgc agcctggacc ttctcggtca
     1201 gatccacaca atcgggcacg tccgacgcca acactccgta gatggcccga tcgatggtca
     1261 gcgtatgctt tgggccgccc tggagagcga gggcggacac tccgtcgcgc tcgatgtccg
     1321 tgaaggtcgc gccgcccgag gcgggcgcgc ggaacacaac gaacacggag cccatggggc
     1381 c
//
LOCUS       DAOSPL010001439         1380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_95699_length_1380_cov_3.477736, whole genome shotgun sequence.
ACCESSION   DAOSPL010001439 DAOSPL010000000
VERSION     DAOSPL010001439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95699_length_1380_cov_3.477736"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PLQ54_20450"
     CDS             complement(<1..860)
                     /locus_tag="PLQ54_20450"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOS95693.1"
                     /translation="MVWAAAKATRARRLCDTTHALAERALSGEHGRAMAPATFGLTQA
                     EQAGYSAEMRCALAIRRIAEQAPLRILPGERIVGSATLREATLHQVPVAGIASTSHTT
                     PGFGRALAMGYAGLRAQVGERLSRPGLDGRGRDLLEAMRVCLDAAGIWHRRHMELLTE
                     LAARSEGTERAEYEAVRAHLARVPECPPEGFREAVQSLWFLFAFQRLCGNWPGIGRID
                     EMLGPYLERDLAEGRLTLDEAREILAHFWIKGCEWTGASDFGGSGDAQYYQNVVLAGV
                     DAEGHEVTNAV"
     gene            1135..1211
                     /locus_tag="PLQ54_20455"
     tRNA            1135..1211
                     /locus_tag="PLQ54_20455"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1169..1171,aa:Met,seq:cat)
BASE COUNT          211 a          503 c          454 g          212 t
ORIGIN      
        1 acggcgttgg tcacctcatg gccctccgcg tccacgcctg cgaggaccac gttctgatag
       61 tattgcgcat cgccggagcc gccgaagtcc gacgcccccg tccactcaca gcccttgatc
      121 cagaagtgcg ccagaatctc ccgggcctcg tcgagggtca gccgtccctc cgccaggtca
      181 cgctccaggt agggcccgag catctcgtcg atgcggccga tacccggcca gttgccgcac
      241 agcctttgga aggcgaacag gaaccaaagc gactggaccg cctcgcggaa tccctccggc
      301 gggcactcgg ggacgcgtgc caagtgggcg cgtaccgcct cgtactccgc gcgttcggtt
      361 ccctccgagc gggcagccag ttcggtcagc agctccatgt gccggcggtg ccagatgcct
      421 gccgcgtcga ggcacacccg catcgcctca agcagatccc ggccgcggcc atcgagcccg
      481 ggccgtgaga ggcgctcgcc aacttgggcg cgcagacccg cgtagcccat cgcgagggcc
      541 cgcccgaaac cgggggtcgt gtgactcgtg ctcgcgatgc ccgccaccgg cacctggtga
      601 agggtggcct cacggagcgt cgccgaaccg acgatccgct ctcccggcag aatccgcagt
      661 ggggcctgct cggcgatccg gcggatggcc agagcgcacc gcatctcggc ggagtagccc
      721 gcctgctcgg cctgggtcag cccgaacgtc gcgggggcca tggcgcgccc gtgctcgccg
      781 gacagggcgc gctcggccag ggcatgtgtg gtgtcacaca gacgcctcgc ccgcgtagcc
      841 ttcgccgcag cccacaccat cgcgcatcgc ccccacagag ccgattgtac tccggtgcat
      901 cccgccgatg ccaacacgcc cggcggcgag cgccgactct cgccgggtgc acgaacctgg
      961 gcaggtatgg tcacggggtg gggcggaccg gcccgggtat cctcctgggc tcgacaccgg
     1021 tagcgcggga ggcctgacgg cttgatgtcg ccacccactg ggcgttctcc agccgcaaaa
     1081 cggcgatgcg ggcttgacat gcgcccccgc atggggtatc ttctccggcg acgacgcggg
     1141 gtggagcagt ctggtagctc gacgggctca taacccgtag gtcgcaggtt caaatcctgc
     1201 ccccgctacc atacgttggg aaacctgagc cgggacctgt cggagatgaa gttggatctc
     1261 ccgggtaccc ggctcgagta ctatctggga cgcgaactgg cggacgacat cccttcgcgc
     1321 cttgctcgtc tccgcgttca gccctcgtag ggcctcggcc cgcagtgcta gcagctcgct
//
LOCUS       DAOSPL010001440         1378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_95914_length_1378_cov_3.247166, whole genome shotgun sequence.
ACCESSION   DAOSPL010001440 DAOSPL010000000
VERSION     DAOSPL010001440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95914_length_1378_cov_3.247166"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PLQ54_20460"
     CDS             complement(<1..301)
                     /locus_tag="PLQ54_20460"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOS95694.1"
                     /translation="MRATGLAAVAEALGVDCPSEASGARVMGVVHDTRLIEGGELFFA
                     LPGEQADGHSFIGEAFARGAVAAVSNRPSEARGPVLMVRDPLQALGRLCRWYRSSL"
     gene            complement(298..>1378)
                     /locus_tag="PLQ54_20465"
     CDS             complement(298..>1378)
                     /locus_tag="PLQ54_20465"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOS95695.1"
                     /translation="GLLTTLEVRIGPGRSAVAGRTTPEASDLQEALRAMVDAGAQHAT
                     MEVSSHALVLGRVAGTLFGCVAFSNLSHDHLDFHGTPENYYEAKRRLFTSEYRGIGSC
                     PAVVNTDDEWGSRLAREADRPVLRVGMNDGADVRARRWEIAADGTRIELATPVGVLDI
                     RSPLRGLYTVSNVALAVGIGVVLDVPAEIIVRGVESLAAVPGRLEPVTGREGQPLVLV
                     DYAHTPAGLRNVLRSVREMRPERVIVVFGCGGDRDRTKRPEMGAIAARLADVAVVTSD
                     NPRSEDPEAIIDEIVGGMPVSAARRITDRREAIFEAVAMAGPKDIVVIAGKGHETYQL
                     FNGYQIDFDDRQVARQALEAAGGSL"
BASE COUNT          241 a          534 c          398 g          205 t
ORIGIN      
        1 ggagagagga ccggtaccat cggcacagcc gccccaaagc ctgtagcggg tcgcgcacca
       61 tgaggaccgg cccccgcgcc tcggatggcc ggttcgacac ggccgccacc gcgccgcgcg
      121 cgaacgcctc gccgataaac gagtgcccgt cggcctgctc gccaggtagt gcaaagaaca
      181 gctcgccgcc ctcgatcagg cgggtgtcat gcacaacccc catcactcgg gcccccgacg
      241 cctcgctcgg gcagtctaca cccagtgcct ccgctaccgc cgccagtcca gtggccctca
      301 cagcgaacct ccggctgcct cgagagcctg gcgggccacc tgccggtcat cgaagtcgat
      361 ctgatacccg ttgaacagct gataggtctc atgtcccttg cccgcgatca ccacgatgtc
      421 cttggggccg gccatcgcca cggcctcgaa gatggcctcg cgtcgatccg tgatacgccg
      481 ggctgccgag accggcatcc cgccgacgat ctcgtcgatg atggcctcag ggtcctcgga
      541 ccggggattg tccgaggtca ccacggcgac atcagccagg cgagccgcaa tagcccccat
      601 ctccggacgc ttcgtccgat cgcggtcacc tccgcagccg aacaccacga tcacccgctc
      661 gggccgcatc tcacgtaccg agcgcagcac attgcgcaaa ccggccggcg tgtgcgcgta
      721 atccaccagc accagcggct gcccttcccg tccggtgacc ggctcgagac gccccggcac
      781 cgcggccaag gactccaccc cgcgtacgat gatctcggcc ggtacgtcca ggaccacgcc
      841 gatgccgacg gccagggcca cattcgatac cgtgtacaac cccctcagag ggctccgtat
      901 gtcgagaaca cccacaggtg tcgccagctc gatgcgcgtg ccgtcggccg caatctccca
      961 acgacgggcc cgcacgtccg cgccatcgtt catccccacc cgcagcacgg gccgatcggc
     1021 ctcacgcgcc agccgactcc cccattcgtc gtcagtgttc accaccgcgg ggcaagaccc
     1081 gatccctcgg tactctgatg tgaacagccg gcgcttggcc tcgtagtagt tctccggcgt
     1141 tccgtgaaag tccaaatggt catgcgacag attgctaaat gcgacgcacc caaacaacgt
     1201 gccagccacg cggcccaata ccagcgcgtg cgatgacact tccatcgtcg cgtgctgtgc
     1261 tcccgcgtcg accatggccc ggagcgcttc ttgcagatcg ctcgcctcgg gagtggtccg
     1321 cccggccacc gcagaccggc ccgggccgat gcgcacctcg agcgtggtga ggagaccc
//
LOCUS       DAOSPL010001441         1376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_96164_length_1376_cov_2.242241, whole genome shotgun sequence.
ACCESSION   DAOSPL010001441 DAOSPL010000000
VERSION     DAOSPL010001441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96164_length_1376_cov_2.242241"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1376)
                     /locus_tag="PLQ54_20470"
     CDS             complement(<1..>1376)
                     /locus_tag="PLQ54_20470"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOS95696.1"
                     /translation="RSSATAEDLPEASFAGQQETFLSVEGHEALIGAIGACWVSLHGE
                     RARAYRLEQGIAEDDLAMAVVVQRMVAARAAGVLFTIDPSTGDANRMVVEAVAGLGEA
                     LVSGHAIPQRFVIRRTDVSLQEGPAGAPLLDRGQLSALCELGARIEESLGGPQDIEWA
                     IDDDGPKILQARAVTGLGRQAELLALIDEERQRLSEEAGGVPTVWSSYQAAEVVHAPL
                     PMTFAVLSRWLSHAGGHGAAHRALGYSPEDTGRSVLELICGRAYYNLNEEARSYLTGV
                     PYAPNVAAIKAHPPSAAEAVPELQATQAGCGFIFALPAVMWRLSRAVSVQRRLKATYV
                     TELTERVFPEFESYIERERAVDLSRLDARELVDTILRWIDHLACDFGRHAIQPSILAG
                     EALLGVQRTLSRSMDADAAKQVARQLVSGDEGNRTTEAAYRLWQVGNGEVSLEAFLEE
                     FGHRAAGE"
BASE COUNT          237 a          479 c          458 g          202 t
ORIGIN      
        1 gctcgccggc cgcccgatgc ccaaactcct ccagaaatgc ctccagcgac acctcgccgt
       61 tgccgacctg ccacagccgg taagccgcct cggtcgtccg gttgccctca tcgcccgaca
      121 caagctggcg ggctacctgc ttcgcggcgt cggcgtccat cgaccgagac agcgtacgct
      181 gtacaccgag cagagcctcg cccgccagga tggagggctg aatcgcgtgc cgaccgaagt
      241 cgcaagcgag gtggtcgatc cagcgaagaa tggtgtccac aagttcccgc gcgtcgagcc
      301 gcgagaggtc gaccgcccgc tcgcgctcga tgtaggactc gaactcgggg aagacgcgct
      361 ccgtgagctc ggtgacgtac gtcgccttga gccggcgctg gaccgagacc gctctggata
      421 gtctccacat caccgcgggc agagcgaaga tgaacccaca gccagcctgc gtggcctgaa
      481 gctcgggcac ggcctcggcg gcagacgggg gatgcgcctt gatggctgcg acgttgggcg
      541 cataggggac gccagtcagg tatgaccggg cttcctcgtt gaggttgtag taggcacgac
      601 cgcagatgag ctcgaggaca ctgcggcccg tgtcttcagg cgagtagccc agcgcgcggt
      661 gggcggcgcc gtgacccccg gcatgcgaga gccatcgaga gaggacggcg aaggtcatgg
      721 gcaggggtgc gtggacgacc tcggccgcct gatagctgct ccagaccgtg ggtacgccgc
      781 ccgcctcctc gctgagccgc tggcgctcct cgtcgatgag tgccagcagc tccgcctgtc
      841 gcccgaggcc agtcactgcg cgtgcctgca gaatcttggg cccgtcgtca tcgatagccc
      901 attcgatgtc ctgcggcccg cccagcgact cctcgatccg cgccccaagc tcacacaggg
      961 ccgaaagctg accccggtcg aggagaggag cgccggccgg tccctcctgc aacgaaacgt
     1021 cggtccggcg gatcacgaac cgctggggta tggcgtgacc ggaaacgagg gcttcgccca
     1081 gtccggcgac ggcctcgacc accatccggt tcgcgtcgcc agtcgacgga tcgatggtga
     1141 acagcacgcc agccgcacga gcggccacca tgcgctgcac gaccacggcc atcgccaggt
     1201 cgtcctcggc gatgccctgc tccaagcgat aggcacgggc acgctcgccg tggagagaaa
     1261 cccagcaggc accgatcgcg ccgatgagtg cctcgtggcc ctcgacgctc agaaaggtct
     1321 cttgctgccc agcgaaactg gcctcgggga ggtcctcggc cgtggcagag cttcgc
//
LOCUS       DAOSPL010001442         1375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_96234_length_1375_cov_3.233333, whole genome shotgun sequence.
ACCESSION   DAOSPL010001442 DAOSPL010000000
VERSION     DAOSPL010001442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96234_length_1375_cov_3.233333"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="PLQ54_20475"
     CDS             complement(<1..1104)
                     /locus_tag="PLQ54_20475"
                     /inference="COORDINATES: protein motif:HMM:NF015119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HOS95697.1"
                     /translation="MPRVHVRPLIPLGHSLSVRLMGTETEYGIYVDGVEPGGLDQESA
                     RLVEAVPPPFAARWDYSAETPRRDLRGFTVNALARDARDAAYDAAVGEASRAAPADRV
                     LANGSRLYNDHAHPEYSTPECASLWDLVAHEKAGEQIVAACASRRAADLGRRVALYKN
                     NTDFHGMSYGAHENYLLPRSVPDEVLLGGLLPFLVSRQAFAGAGKVGVERHRAPKVRY
                     QLSQRADFFDTDVGVDTLARRPIVNTRDEPHTDASQYRRLHVICGDANMSEYAMALKV
                     GVTALVLETLAAGRAPAIRLAKPVEAVRRISWDPAFEYRAETVDGGRLTALEIQRVYL
                     DAASALELPSGSEGEWVLRQWAAMLDALATDPSS"
     gene            complement(1135..>1375)
                     /locus_tag="PLQ54_20480"
     CDS             complement(1135..>1375)
                     /locus_tag="PLQ54_20480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HOS95698.1"
                     /translation="AKDLAIRRAIEKGNPAEGMQWADLEQAIDLEFRENEIFPKSDVV
                     EDWLKLIDYEPENVVTVKPIRPDRGRGRAARHTVI"
BASE COUNT          231 a          516 c          418 g          210 t
ORIGIN      
        1 agacgacgga tcggtcgcca gtgcgtccag catcgccgcc cactgccgga gcacccactc
       61 tccctccgaa ccactcggca actccagcgc cgacgcagcg tccaggtaga cccgctggat
      121 ctcgagggcc gtcaggcggc ccccgtcaac cgtctcggct cggtactcaa acgccgggtc
      181 ccaggagatc cgccgcaccg cctcgaccgg cttggccagc cgaatcgccg gtgctcgccc
      241 cgcggccagc gtctccagaa ccagggccgt gacccccacc ttgagcgcca tggcgtactc
      301 gctcatgttc gcatcgccac agatcacgtg cagccggcgg tactggctgg catcggtgtg
      361 tggctcatcg cgcgtgttga cgatggggcg ccgggccagc gtatcgaccc ccacatcggt
      421 gtcgaaaaag tcggcccgct gcgagagctg ataccggacc ttcggagctc ggtgccgttc
      481 cacccccacc ttgccggcac ccgcgaacgc ctgccgcgaa accaggaacg gcaacagccc
      541 gccgagcaac acctcgtcag gcaccgaccg cggcaacaga tagttctcgt gggccccata
      601 gctcatgcca tggaagtcgg tattgttctt atacagcgcc accctgcggc ccaggtccgc
      661 ggcccgccgg gaggcgcaag cggccacgat ctgctcgccc gccttctcgt gcgccaccag
      721 gtcccaaagc gatgcacact ccggtgtcga gtactcggga tgggcgtggt cgttgtagag
      781 ccgggacccg ttggccagaa cgcggtcggc cggggcggca cgcgacgcct ccccaaccgc
      841 cgcatcatag gcggcatccc gagcgtcgcg cgccagcgcg ttcaccgtga aaccgcgcag
      901 atcccgccgg ggtgtctcgg ccgagtagtc ccaccgtgcc gcgaagggag gaggaaccgc
      961 ctcgaccagc cgggcagact cctggtcgag gccccccggc tcaacgccgt caacgtagat
     1021 gccatactcg gtctccgtac ccatgagccg caccgacagt gagtgaccca gggggatcag
     1081 gggcctgaca tgcactcgag gcaccaggga atgctccctt cccaactcgc cgggctagat
     1141 caccgtatgc cgggcggccc gaccgcggcc ccgatccggc cggatcggct tcaccgtgac
     1201 cacgttctcg ggctcgtagt cgatcagctt cagccagtcc tccaccacat cagacttcgg
     1261 gaagatctcg ttctcgcgga actccaggtc gatggcctgt tccaggtccg cccactgcat
     1321 cccctcggcg gggttgccct tctcgatggc gcggcgaatc gccagatcct tcgcg
//
LOCUS       DAOSPL010001443         1375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_96237_length_1375_cov_3.160606, whole genome shotgun sequence.
ACCESSION   DAOSPL010001443 DAOSPL010000000
VERSION     DAOSPL010001443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96237_length_1375_cov_3.160606"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PLQ54_20485"
     CDS             <1..278
                     /locus_tag="PLQ54_20485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOS95699.1"
                     /translation="VWSLGEPVELTNREYALLEFFIRRPRRLLSRAEIADHVWGMDFE
                     SGTNVIDVYVSHLRRKLDAGRPGSLFRAVKGAGYMMVDPATDNATHA"
     gene            352..>1375
                     /locus_tag="PLQ54_20490"
     CDS             352..>1375
                     /locus_tag="PLQ54_20490"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOS95700.1"
                     /translation="MVGRSLQQTIDAALAAEVSELAAFVQDYGEAVLHRSSSPPVRFR
                     FAAGYFEVTRPSGEIVMASEALGGVRLPATPTAASEGPNQKTALRTQQVGPLGRLRIA
                     ARTVDAPGLGVVTIQTAMPMHQTETTVRRLAPVLALCAVLACCGTTAAGWLVAAWVVA
                     PVRHVARTARRVSGEDLGARVVLGRSEEEIRELVDTFNGMLDRLQAALEQARRFSADA
                     SHELRTPLAVIRGEVEVALERDRSESEYRALLRSVLEEVEALSRLVSDLLDLSRADAG
                     ATGLSCELVDLAEIAESTCDRFETRFAEAGGSLIRRWSGSFPIVADPVRVRQLLGNLL
                     DNAAKYA"
BASE COUNT          190 a          424 c          497 g          264 t
ORIGIN      
        1 gcgtgtggtc gctcggtgag cccgtggaac tgacgaaccg cgagtatgcc cttctggagt
       61 tcttcatccg ccgtccgcgc cgcctgctga gccgtgcaga gattgcggac cacgtgtggg
      121 ggatggattt cgagtcgggg acgaatgtca ttgatgtcta cgtcagccat ctacgccgca
      181 agcttgatgc tggaaggccg ggctcgctgt tccgggcggt gaagggtgcc gggtacatga
      241 tggtggaccc ggccacggac aatgccacgc acgcttaggg ggcgcatcgc cctcctgtat
      301 gcctctgtcg ccggcgccac agtcctggcg gtgtgcggag ttgtcttcgc catggtgggg
      361 cggagcctgc agcagacgat cgacgcggca ctcgcggccg aagtcagtga gctggccgct
      421 ttcgtgcagg actatggtga agccgttctc caccggtcgt cttcgccacc ggtacgtttc
      481 cgcttcgcgg ccggctactt cgaggtgact cggccctcgg gcgagatcgt catggcctct
      541 gaggcgcttg gtggggtccg gctcccggca acccccactg ccgcgtctga ggggcccaac
      601 cagaagacgg ctctccggac ccagcaggtg ggcccgctgg ggcgcttgcg catcgcggct
      661 cgcactgtgg atgcccccgg cctgggtgtg gtgaccatcc agacggcgat gcccatgcat
      721 cagaccgaga cgacggttcg ccgcctggct cccgtgctgg cgctgtgcgc agttctggcg
      781 tgttgcggga ccacggccgc cgggtggctc gtggccgctt gggttgtcgc ccccgtccgt
      841 cacgtcgccc ggacggcccg ccgcgtgtcg ggggaggacc tcggcgcgcg tgtggttctg
      901 ggccggtctg aagaggagat ccgggaactc gttgacacgt tcaacggcat gctggatcgc
      961 ttgcaggcag ctcttgagca ggcgcgccgg ttctcggccg acgcatcgca cgaactgcgt
     1021 actcctctgg cggtgatccg gggcgaggtc gaggtggcgc tggagaggga ccgcagcgag
     1081 agcgaatacc gggccttatt gcgcagcgtg ttggaggaag tcgaggcatt gtctcgcttg
     1141 gtcagcgacc tcttggacct gtctcgcgca gatgccgggg ccactgggct gagctgcgag
     1201 ctggtggatc tggcggagat tgccgagagc acctgcgacc gtttcgaaac gcggttcgct
     1261 gaggcgggcg gatccctcat ccggcgctgg agcggttcgt tcccgatcgt tgcagatccc
     1321 gtacgggttc gccagctgtt ggggaacctg ctcgacaacg cggccaagta cgctg
//
LOCUS       DAOSPL010001444         1375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_96247_length_1375_cov_2.897727, whole genome shotgun sequence.
ACCESSION   DAOSPL010001444 DAOSPL010000000
VERSION     DAOSPL010001444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96247_length_1375_cov_2.897727"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="PLQ54_20495"
     CDS             complement(<1..933)
                     /locus_tag="PLQ54_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOS95701.1"
                     /translation="MWRSPALLVLASSVATVAWLVAAGGQETEGQANITQAPVSQWLR
                     EPQPLPDADAQSEADMKAYTETIPGTNITFRMVPIPGGTFTMGSPEGEAGRKPSEGPQ
                     FPVTVAPFWMATCEVTWDEYDLWSAGIDKRRRAASGESTPWDLLADAITKPTPPYVDM
                     TFGMGKEGYPAICMTQLAAKVYCKWLSAKTGRYYRLPTEAEWEYACRAGSTTAYCFGD
                     DPPALGEYAWYFDNSNDTTHPVGQKKPNAWGLHDMHGNVAEWVLDKFTPEGYEVEAGQ
                     AQQDYVNVPDKLYPRVARGGSFEEHAEMLRSAARR"
     gene            complement(1035..>1375)
                     /locus_tag="PLQ54_20500"
     CDS             complement(1035..>1375)
                     /locus_tag="PLQ54_20500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOS95702.1"
                     /translation="QVRLRRAAVATSGDAWQFAILDGQRYSHIVDPRTGMALSGRRST
                     TVVAHDGMTADAWATALNVLDVSEGFAALENVPGAAALTTREEPSGDRSYASRRWCQL
                     VRSGTITPGG"
BASE COUNT          221 a          478 c          437 g          239 t
ORIGIN      
        1 ccggcgcgcg gccgagcgaa gcatctcggc gtgctcctcg aacgagccac cgcgcgcgac
       61 acgcgggtag agcttgtccg ggacattcac gtagtcctgc tgtgcctgtc ccgcctcgac
      121 ctcatagcct tcgggcgtga acttgtcgag cacccactcg gccacattgc cgtgcatgtc
      181 atggaggccc caagcgttgg gcttcttctg gcctaccggg tgcgtggtgt cgttgctgtt
      241 atcgaaatac caggcatact caccgagggc cggcggatca tcgccgaagc agtaggcggt
      301 ggtcgatccc gcccggcagg cgtactccca ctcggcttcg gtcggcaggc gatagtagcg
      361 gcccgtcttc gccgacagcc acttgcagta caccttggcc gcgagctgcg tcatgcagat
      421 ggctggatag ccctccttgc ccatgccgaa ggtcatgtcg acgtaaggtg gagtgggctt
      481 ggtgatggcg tcggccaaca ggtcccatgg cgtggactcg ccactggcgg cgcgccgccg
      541 cttgtcgatg cccgcactcc acaggtcgta ctcgtcccag gtaacctcac aggtggccat
      601 ccagaacggg gcaacggtga cggggaactg aggaccctcg gagggcttcc gtccggcctc
      661 cccttcgggg ctgcccatgg tgaaggtccc gccggggatg gggaccatcc tgaacgtgat
      721 gttggtgcca gggatggtct ccgtgtaggc cttcatgtcg gcctcgctct gtgcgtcagc
      781 atccggcagc ggctgtggtt cccgcagcca ctgcgagacc ggtgcctgcg taatgttggc
      841 ctggccctcg gtctcctgcc cgcctgccgc caccagccag gccacggtgg cgacggaact
      901 cgcaagaacc agcagtgcgg ggctccgcca caccccacac atccttcgtg catctctcac
      961 cctgtcacga ctccttcccc ataccaccga aacgacagaa ccgttctggc ggcgaccgcg
     1021 gatccgcacg atggtcagcc gcccggagtg atcgtgcctg atcgcaccag ttgacaccag
     1081 cgccgcgagg cataggaccg gtcgccactc ggctcctcac gcgtcgtcag cgccgccgcg
     1141 cccggcacgt tctcgagggc ggcgaagccc tcactcacgt ccaagacgtt cagcgccgtg
     1201 gcccaggcat cagctgtcat gccatcgtgg gccaccacgg ttgtggaccg gcgccccgag
     1261 agggccatgc cggtccgcgg atccacaatg tgcgagtacc gctgaccatc caggatggcg
     1321 aactgccagg cgtcgcccga agtggccacg gcggcgcgac gaagccgaac ttggt
//
LOCUS       DAOSPL010001445         1374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_96407_length_1374_cov_1.970432, whole genome shotgun sequence.
ACCESSION   DAOSPL010001445 DAOSPL010000000
VERSION     DAOSPL010001445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96407_length_1374_cov_1.970432"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PLQ54_20505"
     CDS             complement(<1..591)
                     /locus_tag="PLQ54_20505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95703.1"
                     /translation="MRALLVRWSASAVLSVLLAVVIGGCGDGGQVINPPVDNDQQLLV
                     GTGSANGYVYTPVRVRASDLVVCELPLTEAEQEAQGLEPLAGGIATMDTGQKCRTGRS
                     GTYSLVGVPAGSRVISFADRTGASLGSGVPVNVRTNGTTSGTRDPALTSTDPGACFGY
                     LLGRPRMGSPIGGAAGLTWTDVRFAPSDLSGGLGELL"
     gene            complement(730..1167)
                     /gene="ruvX"
                     /locus_tag="PLQ54_20510"
     CDS             complement(730..1167)
                     /gene="ruvX"
                     /locus_tag="PLQ54_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006445508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOS95704.1"
                     /translation="MRILAVDYGTRRIGLAVSDPLGVVASPHGFVDVADDGSHLDTIA
                     QLCTDLEVGRIVVGLPLHMSGEAGASAEAARGLVEALADRVTVPVELFDERLTTTQAE
                     RALLECNVRRGRRKQVVDGVAAAVLLGAYLDRARQLSPDCDVG"
     gene            complement(1168..>1374)
                     /locus_tag="PLQ54_20515"
     CDS             complement(1168..>1374)
                     /locus_tag="PLQ54_20515"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOS95705.1"
                     /translation="HPSNPWQPCPWFTRNYGFMSPTPLNWLEVPGMALPAGSQISLRY
                     RVVAYAGTPEEAGLAALYDGWAAG"
BASE COUNT          233 a          491 c          451 g          199 t
ORIGIN      
        1 caggagttcg cctagcccgc cgctgaggtc cgagggtgca aaccggacat cggtccatgt
       61 caggccggca gcgcccccga tgggcgatcc catgcggggc cgcccgagaa ggtagccaaa
      121 acacgccccc gggtcggtgc tcgttagcgc ggggtcgcga gtgcccgagg tggtgccatt
      181 cgtacgcacg ttaacgggca cgcccgaccc cagcgaggcg cccgtcctgt cggcgaagga
      241 gatcacgcga ctcccggcgg gcacccctac cagcgagtag gtgcctgaac gcccggtgcg
      301 gcacttctgg cccgtgtcca tggtcgcgat gccaccggca agcggttcga gcccctgagc
      361 ttcctgctcg gcctcagtaa gcgggagctc gcagactacc agatccgacg cccgaacgcg
      421 cacaggcgtg tacacgtatc cgttggcgct gccggtgccc accagtagtt gctgatcgtt
      481 gtcgactggc ggattgatca cctggccccc gtcaccacag ccgccgatga cgaccgccag
      541 gagaacggag agaacggcag acgcgctcca tcggactagc aatgctctca cctgattcct
      601 gccctcacgc aggcacgcac ctcacccggg acacccattc ggatgccggc acacgccgac
      661 cccatggaac tcggacagca ataggtttcg ctcccggcgc cagggaccct cctggtccac
      721 agggcgcgct cacccgacgt cgcagtcagg agacagctgc ctggcccgat ccaggtatgc
      781 gccgaggagt acagcggccg cgacgccatc gactacctgc ttgcggcgcc cgcgccggac
      841 attgcattcc aggagggccc gttcggcctg ggtcgtcgtc aggcgctcgt cgaagagctc
      901 cacgggcacc gtgactctgt cggccagggc ctcgacaagc ccccgcgcag cctcggccga
      961 tgcgccggct tcgcccgaca tgtgcagcgg cagcccgacc acgatgcgcc ccacttcgag
     1021 atcggtgcag agctgcgcga tggtgtcgag atgagagcca tcgtccgcca cgtcgacgaa
     1081 gccgtgcggc gatgccacga cgccgagagg gtcgctcacc gccaagccga ttcgccgggt
     1141 gccgtagtcg accgccagga tgcgcaccta tcctgccgcc catccgtcgt agagcgcggc
     1201 caggccggcc tcttcaggcg tgccggcata ggccaccaca cggtagcgca ggctgatttg
     1261 gctgcccgcg ggcagggcca tgccgggcac ctcgagccag ttcagcgggg tgggcgacat
     1321 gaagccgtag ttgcgcgtga accaggggca cggctgccac ggattgctcg ggtg
//
LOCUS       DAOSPL010001446         1373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_96446_length_1373_cov_3.500000, whole genome shotgun sequence.
ACCESSION   DAOSPL010001446 DAOSPL010000000
VERSION     DAOSPL010001446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96446_length_1373_cov_3.500000"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="PLQ54_20520"
     CDS             <1..738
                     /locus_tag="PLQ54_20520"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOS95706.1"
                     /translation="LSYAHSRGVIHRDIKPDNISVSPTDRVKVMDFGVARLATDITVS
                     SPGTVIGSPSYMSPEQIQGGDVDGRTDVFTLGTVLYEMLTGRRLFQGSSVPEVAYKIV
                     HTEPDLGGSFPPRLRALLRRALAKDPRERFATAEQMLTALRDPLLLRWDLAAGDRRED
                     DSALGVPVAPPGRSRVLMIGLVGGYAAAIAGTLIFAGPAAVSLAAGGAAVALTARLAG
                     GARSSALAGWGALCAAVLAAAHTWLDW"
     gene            complement(751..>1373)
                     /locus_tag="PLQ54_20525"
     CDS             complement(751..>1373)
                     /locus_tag="PLQ54_20525"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOS95707.1"
                     /translation="AEELRQPIAIAFDEWGMWLGKDHGGEYTLADGLVAALYLNAFIR
                     HASMVRMANYAQLVNVIPALSTTRDDLFLQATYFPLELFQHHNGPVALDVWCDAETFT
                     SGDHTAPALDVTATLDPGKAQVTLNLVNLDTSEARDVSVECQHGAFKWRAMLSILACD
                     SPDATNGFDAKDRVAVDTFALAPRPDRLTFEAPPHSLCVLRAPLDA"
BASE COUNT          209 a          451 c          478 g          235 t
ORIGIN      
        1 ctgagctacg cccactcgcg cggcgtcatc catcgcgata tcaagcccga caacatcagc
       61 gtctcgccca ccgatcgcgt taaggtcatg gacttcggcg tcgcacgcct ggccacggac
      121 atcacggtat cgagccccgg aacggtaatc ggatcgccca gctacatgtc tcccgaacag
      181 atccagggcg gcgatgtgga tgggcgcacc gacgtgttta ctctgggcac ggtgctgtac
      241 gagatgctca cgggccgacg gctgttccag gggagctccg tgcccgaggt tgcctacaag
      301 atcgtccaca cggaaccgga tctgggcggc agttttccgc ccaggcttcg cgctctgctg
      361 cggcgcgcgc ttgccaagga tcccagggag cgcttcgcga cggccgagca gatgcttacg
      421 gccctccgcg accccttgct cctgcgatgg gacttggcgg ccggggaccg ccgcgaggat
      481 gactcggcgt tgggggtgcc cgtcgcaccc ccgggccgat cgcgggtcct gatgattggc
      541 cttgtcggtg gctacgcggc cgccatcgcg ggcactctga tctttgccgg acccgccgcc
      601 gtatcgctgg cggcgggggg cgccgccgtg gcgctcacgg cacggctcgc gggaggggcc
      661 cggtcgtcgg ccctggccgg atggggcgcc ctctgtgccg ccgtgctggc ggcggcccac
      721 acctggctcg actggtaacc cgcgggatcc tcaggcgtcc agcggcgccc gcaggacgca
      781 cagcgagtgg ggtggcgcct cgaatgtgag gcggtcgggt cgcggagcga gcgcgaacgt
      841 gtcgacggcc acgcgatcct tggcgtcgaa cccgttcgtc gcatccgggc tgtcgcaggc
      901 gagaatgctc agcatggccc tccatttgaa agcgccatgc tggcactcga cagatacgtc
      961 ccgggcctct gacgtgtcga gattcacgag gttcagcgtg acctgtgcct tgccgggatc
     1021 cagtgtggcc gtcacatcca atgccggcgc ggtgtggtcg cccgaggtga acgtttcggc
     1081 atcgcaccag acatccagcg cgaccggtcc gttgtggtgc tggaacagtt cgagcgggaa
     1141 gtaggttgcc tggaggaaca gatcgtcacg ggtggtgctg agggccggga tgacgttcac
     1201 cagctgggcg tagttggcca tgcggaccat tgaggcgtga cgaatgaacg cattgaggta
     1261 aagcgcggcc accaggccat cggccagcgt gtactcgccg ccatggtcct tcccgagcca
     1321 catgccccac tcgtcgaagg cgatggcgat cggctgccgc agctcctcgg ccg
//
LOCUS       DAOSPL010001447         1372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_96560_length_1372_cov_2.990129, whole genome shotgun sequence.
ACCESSION   DAOSPL010001447 DAOSPL010000000
VERSION     DAOSPL010001447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96560_length_1372_cov_2.990129"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..133)
                     /locus_tag="PLQ54_20530"
                     /pseudo
     CDS             complement(1..133)
                     /locus_tag="PLQ54_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377682.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
     gene            complement(478..>1372)
                     /locus_tag="PLQ54_20535"
     CDS             complement(478..>1372)
                     /locus_tag="PLQ54_20535"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95708.1"
                     /translation="GGRFVGALGANGRDGRSPYDDRNPAWLMAGREVDTPAGKRLEWS
                     QPEIVLYDDDPYIRMSYPDLIEEDGRFFLTETQKTVGRVHEVSARITECLFGQREAGT
                     VATEGLTLQWSPGMAATVPMPRLPDLTVRDTARPDMGRRDTRQGFSLDVWVRFGSLER
                     GQVLIDSRAPAGAGIAWLTTPRGTLELVLNDGRSECRWDCDPGLLQVARWHHVSAIID
                     GGPKLILFVVDGVLCDGGEARQFGWGRFSPNLYNVTGGPELRVAPVLVGELAALRIYK
                     RALMVGEAVANWRAGCAHCGP"
BASE COUNT          210 a          478 c          478 g          206 t
ORIGIN      
        1 acacggtccc tgtcggcccc acggcgtcga ggaaaccgtc gatgaccgtg ttgggaccac
       61 cgaccacgct ccccatgcta ctcagggaac tgtgaaacat gaccacatcg cccgggacga
      121 tcccgaccgc cgcacatgca gcggagacgt cgccgcgatg gacctggatg ctcggatcgc
      181 ctggttcatg tggggtagcc atccgcagct tcctctcagt caggccgacg gaccgggggc
      241 gtgtcggttg cgccgggccc gagtgcatgc gcgaccgagc cgcaccgcgc tgaccctatg
      301 ccttctgccg cggcgcaggc gtccctgccc gccatggccc cgggcagggt gattggccag
      361 gaggttggcc cgtggccctg ccggacgggg cagcgcgtgc ttcgtccgcc tccgtcgcac
      421 gggggccgtc ggccgtgggt cgaagggctg gaccatgggg tccaggtgcc tggcgtccta
      481 tggcccgcag tgggcgcacc ccgcgcgcca gttcgccacg gcttcgccca ccatcagcgc
      541 ccgtttgtag atgcgaaggg cagccagctc gccgacgagc accggcgcga cgcggagctc
      601 cgggccaccg gtcacgttgt acaggttcgg gctgaaacgg ccccagccga actgacgggc
      661 ctccccgcca tcgcacagca caccatcgac cacaaacagg ataagcttgg gcccgccgtc
      721 gatgatcgca gacacgtggt gccaacgggc gacctgtagc aggcctgggt cgcagtccca
      781 ccggcactcg gagcgtccat cgttgagcac gagttcgagt gtcccgcgcg gtgtggtcag
      841 ccaggcgatg ccggcgccgg cgggcgcacg actgtcgatc agcacctgac cgcgttcgag
      901 ggagccaaac cggacccaca catcgaggct gaacccttgg cgggtatcgc ggcggcccat
      961 gtcgggccgc gcggtgtcgc gcaccgtcaa gtccgggagg cgaggcatgg gaacggtggc
     1021 cgccatgccg ggcgaccact gcagcgtcag gccctcggtg gccacggtgc cggcttcccg
     1081 ctggccgaac aagcactcgg tgatccgggc ggacacctcg tgcacgcgcc cgacggtctt
     1141 ctgggtctct gtgaggaaga agcggccgtc ctcctcgatc aggtcgggat aactcatccg
     1201 gatgtagggg tcatcgtcgt agaggacgat ctcgggttgc gaccactcga ggcgcttgcc
     1261 ggcgggcgtg tcgacctcgc ggcccgccat gagccaggcg gggttgcggt cgtcatacgg
     1321 actgcggccg tctctgccgt tcgcgccgag tgcgccgaca aagcgtccgc cg
//
LOCUS       DAOSPL010001448         1369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_96859_length_1369_cov_3.592846, whole genome shotgun sequence.
ACCESSION   DAOSPL010001448 DAOSPL010000000
VERSION     DAOSPL010001448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96859_length_1369_cov_3.592846"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1369)
                     /locus_tag="PLQ54_20540"
     CDS             complement(<1..>1369)
                     /locus_tag="PLQ54_20540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95709.1"
                     /translation="GAKAASEQGPDALVSSPANLVANASFEQGDPPSGWTVSSEGAGG
                     AKLCEPSVDTPGRLGERCAKLVIPPDAPLAWAGWHQRVPVKPSTSYVYMGYLKCRDLD
                     GSVTLYAHILDSEGRPVSGTSNVGAGTALSGTRDWTLTSGQFTTGPDAAIVELHLTVN
                     AHGTVWHDGILLAEVVDGTAESLEAARSEDGQTGLAVWQVDPVTKVFPDEIPGPRPRR
                     VELTAARGEREACQLVLRSDTEVERARVSATPPARDGHHLPAPRIERVGFVPIDYPSG
                     YYASRLPAYFRLCPQGAPGSDGWAGEWPDPLIPDGSFALQPGRAQPVWVTVEVPADAE
                     PGEYRCEITIETGEQMVRIPLRIDVLRLTLPRETSIRAIYDLRSGTGQNIHDGRPMKE
                     SLRDWYRFMAERRVCPDSIVPEPVFTYADGKVTMDATEFDEMATLCFDELGMKHMYTP
                     WLFYA"
BASE COUNT          201 a          437 c          508 g          223 t
ORIGIN      
        1 aaggcataga agagccacgg ggtgtacatg tgcttcatgc cgagctcatc gaagcagagg
       61 gtggccatct cgtcgaactc ggtggcgtcc atcgtcacct tgccatcggc ataggtgaag
      121 acgggctcgg gcacgatgct atcggggcag acccggcgct cggccatgaa gcggtaccag
      181 tctcgcaggc tctccttcat gggtcggcca tcgtggatgt tctgcccggt gccgctgcgc
      241 aggtcgtaga tggcgcggat gctcgtctcg cgcggtagcg tgagacggag cacgtcgatt
      301 cgcagcggga tgcggaccat ctgctcgcct gtctcgatgg tgatctcgca gcggtactcg
      361 ccgggctccg cgtcggctgg cacctcgacg gtcacccaca ccggctgggc ccggccgggt
      421 tgcagggcga aggagccatc cgggatgagc ggatcgggcc actcgccggc ccagccgtcg
      481 gagccggggg cgccctgcgg gcacaaccgg aagtaggcgg ggaggcgaga cgcgtagtag
      541 ccgcttgggt agtcgatggg gacgaaacca acgcgctcga tccggggcgc ggggaggtgg
      601 tgaccgtcgc gagcgggcgg cgtcgccgat acgcgggctc tctcgacttc ggtgtcgctc
      661 cgcagcacga gctggcaggc ctcgcgctcg ccgcgggctg cagtgagctc gacgcgcctt
      721 ggccgcggtc cgggaatctc atcggggaac accttggtga caggatcgac ctgccagacg
      781 gccagcccgg tctggccgtc ctcgctgcgc gctgcttcga gtgactcggc ggtgccgtcg
      841 accacctcgg ccagcagaat accatcgtgc cagacggtgc cgtgtgcgtt gacggtcagg
      901 tgcagctcga cgatggcggc atcggggccg gtcgtgaact ggcccgaggt gagggtccag
      961 tctcgcgtcc cgctcagtgc ggtgccggca ccgacgttcg aggtcccgct cacgggcctt
     1021 ccctcggaat cgaggatgtg agcgtagagc gtcacgctgc cgtcgaggtc ccggcacttc
     1081 aggtaaccca tgtagacgta gctcgtgctc ggcttcacgg gcacgcgctg gtgccagccg
     1141 gcccaggcga gaggtgcatc gggcgggatg acgagcttgg cacagcgctc gcccaggcgc
     1201 ccgggggtat cgacggatgg ctcgcacagc ttcgccccgc cggcgccctc agacgagacc
     1261 gtccagccgc tcggcggatc gccctgctcg aagctggcat tggcgacgag gttggcgggg
     1321 gagctgacga gcgcatcggg gccttgttcg gaggcggcct tggcgccgt
//
LOCUS       DAOSPL010001449         1369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_96860_length_1369_cov_3.514460, whole genome shotgun sequence.
ACCESSION   DAOSPL010001449 DAOSPL010000000
VERSION     DAOSPL010001449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96860_length_1369_cov_3.514460"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..296)
                     /locus_tag="PLQ54_20545"
     CDS             complement(132..296)
                     /locus_tag="PLQ54_20545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95710.1"
                     /translation="MKWLKAEQTIDPVFLWARYNPSDECGGSPGGWAECQRNTARDYI
                     RGWRQRNPNV"
     gene            complement(560..880)
                     /locus_tag="PLQ54_20550"
                     /pseudo
     CDS             complement(560..880)
                     /locus_tag="PLQ54_20550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
BASE COUNT          250 a          447 c          423 g          249 t
ORIGIN      
        1 cgcagtaagt gaggtggggc accgaacacg tgccgccttg gcaaaccatc gaccccgaac
       61 gccatccccc ccaacgcaga cagccggagc catggctggc tccggccgtc gttgcggtgg
      121 cacgactcgc gctacacgtt gggattgcgt tggcgccatc cgcggatgta gtcacgtgcc
      181 gtattccgct ggcactcggc ccatccgccc gggctaccac cgcactcgtc cgacgggttg
      241 tagcgcgccc acaggaagac ggggtcaatc gtctgctcgg ccttgagcca cttcacgtgg
      301 ccgtcgagga acgcgtagtt cgcccccgag ttgtggggct gccggaaaca gcctgatccg
      361 gtgttgctgg ggtggcacca gatgtggcgt cgacgttgcc atgacaggtc aggtagatct
      421 gctgggccgg ctcggtgatg ctctcgaaat gggcccagac ggcaatcccc catgtgccac
      481 agaagcggtt gttcactccg tagcttgatc ggatgtcgta cgtactgggt gggttgttct
      541 ccggattcgg cggaaccacc gacgggcaca ccagagcctg ctcgttcttc atatagggct
      601 ggatgatcac gcgccaggtc acccatgtgc cgtcccagca gctgccgttg gtgtccgccg
      661 ggcagttgcc catccggacg cccggaagcg tctggtcgtt atcagacgca tacatgagaa
      721 aagccataat gatctgcttc tggttggact ggcagctggc ctgccgcgcc ttctctcgcg
      781 ccttggcgaa cacggggaag agtatcgccg ccaggatcgc gatgatggcg atgacgacca
      841 ggagttcgat gagcgtgaac cctccaccgc gcttacgcat tcgtgtgtct cctctcgcga
      901 aggacctcgc cgccgtgaac cagagttcga cggcacgaac ggcgccgcgc taccgtcctg
      961 ctccacaacc tctcttgagc cccgcgccac acccaccacc cgagggctcc tgcatcttgt
     1021 acgcggtctt ccccgcgcac ttggaggctc ccaccgacga cgtagcggcg gcgggctccg
     1081 gagggggcga ctgccccctg caaccacaca gcgcggctcc tgtgaacaac gccaagacga
     1141 ccagtgcacc gatcctcatg ctgccctccc ttacgatgac atcattgcct aacggccatc
     1201 ggtcagccca gtgcggacac ccaccacagc acggcacggg aaaggccata gcgcaaagca
     1261 gaagcacaca catgagcctt ctgcgagaaa cgcctgcatc ctgctagggt tgtaagagct
     1321 cagagttggt gtgggtgaag ttggtgtggg cgtggggtgt caggcggag
//
LOCUS       DAOSPL010001450         1369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_96862_length_1369_cov_3.324201, whole genome shotgun sequence.
ACCESSION   DAOSPL010001450 DAOSPL010000000
VERSION     DAOSPL010001450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96862_length_1369_cov_3.324201"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..99)
                     /locus_tag="PLQ54_20555"
     CDS             complement(<1..99)
                     /locus_tag="PLQ54_20555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HOS95711.1"
                     /translation="MGDTRLITSESVTEGHPDKIADQISDAVPDAML"
     gene            complement(171..806)
                     /gene="gmk"
                     /locus_tag="PLQ54_20560"
     CDS             complement(171..806)
                     /gene="gmk"
                     /locus_tag="PLQ54_20560"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOS95712.1"
                     /translation="MSHPDSADTRCRPIVFVVTGPSGSGKGTVLSRVVCPGMGLRLAV
                     SATSRAPRPTDADGDYHFVTRGEFEATIAAGGFLEYAEFCGELYGTPRSEIERAADEG
                     SDLVLEIEVQGARQIRRVCPRAVLVMLLPPTREECLRRLRGRGSETEERIQRRMAAYD
                     REILAVGEFDYVVWNVEVDAAVCDLKSIIRAERLRTHRQLAAGQAAPRGEG"
     gene            838..>1369
                     /locus_tag="PLQ54_20565"
     CDS             838..>1369
                     /locus_tag="PLQ54_20565"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HOS95713.1"
                     /translation="MSGVRETRDLPSYNGPTAMVFDSAVTACCVAEIAHALVGGRVVS
                     VRQPRRLEVALEVESAQGSSWLIASAEAQYARVHLGRAPAQRDPAQFPFGIFAAKHLR
                     GARVLGVRQIGFDRVVRIDFEPRNRRLVDPVRALVVEVMGKHSNLILLNNVDMVLEAA
                     KHITADMSRVRTVQPHD"
BASE COUNT          214 a          477 c          443 g          235 t
ORIGIN      
        1 gagcatggcg tcgggtaccg cgtcggagat ctggtccgct atcttgtcgg ggtggccctc
       61 ggtgaccgac tccgacgtga tcagtcgcgt gtctcccatg tccctgcaca gcctccaatg
      121 cgtgcgccgg tcccggcgcg agacgagtgg gttccactgt ggcccctcgt ctatccctcg
      181 ccgcggggtg ccgcctggcc cgcggccagc tgccgatgcg ttctcaggcg ttctgcccgt
      241 atgatactct tgaggtcgca cacggcggcg tcgacctcga cgttccacac gacatagtcg
      301 aactcgccga cggccaggat ctcacgatcg taggcggcca tgcggcgctg aatccgttct
      361 tcggtctccg atccgcgccc ccggagccgg cgcagacact cctcacgcgt gggcggcagg
      421 agcattacca gtaccgccct cgggcataca cgccgtatct ggcgcgctcc ctgcacttcg
      481 atctcgagaa ccagatccga cccctcatcc gccgcccgct cgatctcgga ccgaggagta
      541 ccgtacagct ccccgcagaa ctccgcgtac tcgagaaacc ccccggcggc gatggtcgcc
      601 tcgaactcgc ctcgggtgac gaaatggtaa tcgccatcgg cgtccgtggg gcggggcgct
      661 cgcgaggtgg ccgacaccgc gaggcgcagc cccataccag ggcacaccac ccggctgagc
      721 acggtgccct tgccagatcc agagggaccg gtcaccacga agacgatcgg gcggcaccgg
      781 gtatcggccg aatctgggtg gctcatagag aagtcattct agcacaaggt cccggcggtg
      841 tcaggcgtcc gcgagacccg cgatctgcca tcgtataatg ggcccactgc catggtgttc
      901 gactctgccg tcacagcctg ttgtgttgcc gagatcgcgc acgccctggt cggcgggcgc
      961 gtcgtcagcg tgcgtcaacc ccggcggctc gaggtggccc tggaggtcga gtcggcccaa
     1021 ggctcgagct ggctcattgc gtcagccgag gcgcagtatg cgcgcgttca cctgggccga
     1081 gcgccggctc agcgtgaccc tgcccagttc ccgttcggta tcttcgccgc caagcacctg
     1141 cgcggggcgc gggtccttgg ggtccggcag atcggcttcg accgcgtcgt ccggatcgac
     1201 ttcgaaccca ggaaccggcg gctggtggat ccggtgcgtg ctctggtcgt cgaggtcatg
     1261 gggaagcaca gcaatctgat cctcctcaac aatgtggaca tggtcctgga ggccgcgaag
     1321 cacatcaccg cggacatgag ccgggtccgg accgtgcagc cccacgacg
//
LOCUS       DAOSPL010001451         1369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_96877_length_1369_cov_2.808219, whole genome shotgun sequence.
ACCESSION   DAOSPL010001451 DAOSPL010000000
VERSION     DAOSPL010001451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96877_length_1369_cov_2.808219"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PLQ54_20570"
     CDS             <1..604
                     /locus_tag="PLQ54_20570"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOS95714.1"
                     /translation="VANVHAHIDNLRQHGVPVVVAVNRFGQDSDDELSLLCELVEAHG
                     APCEVTDGFAEGSRGAMRLAERVLELVDSSSANFHTLYPDEMGLTEKVETIATRVYGA
                     DGVDFLPAAARALSRLEELGLGRLPVCMAKTQYSLTDDPKIVGRPRGFRITVRDAYPS
                     TGAGFVVCLTGEVMTMPAMSRTPAAIGMDIAEDGTITGLF"
     gene            730..>1369
                     /locus_tag="PLQ54_20575"
     CDS             730..>1369
                     /locus_tag="PLQ54_20575"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOS95715.1"
                     /translation="MKPDADHAAGATPKSLHDGWGSLTGECNAPFASLAAAFKAEGRF
                     EEARAAFEKAIELEPDRLDYYCGLAMVQRELNSSREAADTLRHALAMDPGHDPARQML
                     AEVYLEMGIYTGVIEEARTLLARSPRNAPALAILAAACQETGDMGVAIASVEQLVRLC
                     PRDAHNHYVLGSLYRQRGDLGRALERFERTVELAPDSQLADLAQSEIQSMDSM"
BASE COUNT          223 a          422 c          492 g          232 t
ORIGIN      
        1 cgtggccaac gtgcacgcgc acatcgacaa cctgcgtcag catggggtcc ccgtggtcgt
       61 ggccgtgaac cggttcgggc aggactcgga tgacgagctg agcttgctgt gtgagctggt
      121 ggaagcccac ggggcgccct gcgaggtgac ggacggcttc gctgagggat ctcgtggcgc
      181 catgcgactg gcggagcgtg tgctggagct ggtggactcg agctctgcga actttcacac
      241 cctttacccc gatgagatgg gattgacgga gaaggtggag acgatcgcca cccgcgtgta
      301 tggcgcggat ggtgttgact tcctgccagc ggcggcgcgc gcgttgtcga ggctcgagga
      361 gctgggactg ggccgcctgc cggtatgcat ggccaagacg cagtactcgc tcaccgacga
      421 cccgaagatc gtggggaggc cacgtgggtt tcgcatcacc gtgcgcgatg cgtacccgag
      481 tactggggcg gggtttgtcg tgtgtctgac tggggaagta atgacgatgc cggccatgtc
      541 gcggacgccg gccgccatcg gcatggacat cgccgaggac ggcacgatca ccggcctgtt
      601 ctagttcccg caggttcggc aggaacggag gggccggcgt cgaatgtgtg actggcgtac
      661 atccgaggga gacaggggtc ggcgtggcgg gcccctcagg actccccacc cgggttgtgg
      721 gatgcggcca tgaagccaga cgcggaccat gcagcaggcg cgacgcccaa gtcgctgcac
      781 gacggctggg gctccctgac cggcgagtgc aatgcgccct tcgcttcgct cgctgcggca
      841 ttcaaggccg agggtcggtt cgaagaggcc cgcgccgcct tcgagaaggc cattgagctg
      901 gagcccgatc gcctggacta ctactgcggt ctggccatgg tgcaacggga gctcaatagc
      961 agccgagagg ctgccgacac gctgcgccac gccctcgcca tggaccctgg acacgacccg
     1021 gctcggcaga tgctcgctga ggtctacctg gaaatgggca tctacaccgg cgtgattgag
     1081 gaggcccgca cgctgctggc gcggagccct cggaacgctc ccgccctcgc gatcctggct
     1141 gccgcgtgtc aggagaccgg tgacatgggc gtggcgatcg cgtctgtcga acagctcgtg
     1201 cggctgtgcc cgcgcgatgc ccacaaccac tacgtgctgg gatcgctcta ccgtcagagg
     1261 ggcgacttgg gccgggcgct tgagcgtttc gagcgcaccg tcgagcttgc gcccgactcg
     1321 caactcgccg acttggcgca gtcggagatc cagtccatgg atagcatgc
//
LOCUS       DAOSPL010001452         1368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_96960_length_1368_cov_3.208682, whole genome shotgun sequence.
ACCESSION   DAOSPL010001452 DAOSPL010000000
VERSION     DAOSPL010001452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96960_length_1368_cov_3.208682"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PLQ54_20580"
     CDS             complement(<1..706)
                     /locus_tag="PLQ54_20580"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOS95716.1"
                     /translation="MGRPDESAEALSGVLRIGPERLASIIEFLPDPTFAIDAKKRVIA
                     WNRACEALTGVHRQTVLGQGDYAYAIPFFGHRRPILIDLLDLPDPETAAGYKYVQRQG
                     DLLYAESFIPRLRDGQGAYVWGVAGPLYDENGHRCGAVEVIRDITERRRIEQALRESE
                     VRYRTLFETASDAILLRRDDRFIDCNPRALALFGCTRDEIIGSTIEALSPPTQPDGVP
                     SADRVRERVQRALHEGP"
     gene            complement(793..>1368)
                     /locus_tag="PLQ54_20585"
     CDS             complement(793..>1368)
                     /locus_tag="PLQ54_20585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95717.1"
                     /translation="PDALWRRKGLVDGDNSGSAAFDWAAMPGATPEDIMARVSLPLAE
                     DFYFPGGGNCVSFTSPGGIEGIAARMAYSGEQNIFSLIWDEAVTVDLPEKLSLAVANT
                     STPIWPHTYVVPKYASMGEYKQFAPANHFHMTWGLKPAVLEYWMDLAHVLSVTPWQAR
                     PAYIEGVDRPTPLLHLLNGGEVATKLRLAGR"
BASE COUNT          208 a          430 c          505 g          225 t
ORIGIN      
        1 ggggcccttc gtggagggcc cgctggaccc gctcccgcac gcggtcagcg gacggcacgc
       61 cgtcgggctg ggtggggggc gagagggcct cgatggtgga gccgatgatc tcgtcccggg
      121 tgcatccgaa cagggcaagg gcccgtgggt tgcagtcgat gaagcggtca tcacgccgta
      181 gcagtatggc gtcgctggcg gtctcgaaca gcgtgcggta ccggacttcg ctctcacgga
      241 gcgcctgttc gatccgtctg cgctcggtga tgtcgcggat gacctcgacg gcgccgcaac
      301 ggtggccgtt ctcgtcatac agggggccgg ccacacccca cacgtacgcc ccttgcccgt
      361 ctctgaggcg agggatgaat gactcggcat agaggaggtc gccctggcgc tggacgtact
      421 tgtagccggc cgcggtctcc ggatcgggga gatccaggag gtcgatcagg atgggccgtc
      481 ggtgcccgaa gaaggggata gcgtaggcgt agtctccctg gccgaggacg gtctgcctgt
      541 ggacgcccgt tagcgcctca caggcgcggt tccatgcgat gacccgcttc ttcgcgtcga
      601 tggcgaaggt tggatcgggc aggaactcga tgatcgaggc cagtcgctcc ggcccgatcc
      661 ggagcacacc agatagggcc tcggcgctct cgtccggccg acccatgcca ctccacctcc
      721 gcgcgccccc gggggggccg cccggccaca tggaggcgcc gggcgggctc cggtcgcaga
      781 acgtgggtgt tcctaccgcc ccgccaatct gagcttggtg gccacctcgc cgccgttgag
      841 tagatgcagc aacggagtgg ggcggtcgac cccctcgatg taggccgggc gggcctgcca
      901 cggcgtgacg gagagcacat gggccaggtc catccagtac tcgagcaccg ccggcttgag
      961 gccccaggtc atgtggaaat ggttggcggg agcgaactgc ttgtattcgc ccatgctggc
     1021 gtacttgggc acgacgtagg tgtgcggcca gatgggtgtg gacgtgttgg ccacggcgag
     1081 ggagagcttt tcgggcaagt cgaccgtgac ggcctcatcc cagatgaggg agaagatgtt
     1141 ctgctcgccg ctgtaggcca tccgcgcggc gatgccctcg ataccgccgg gggaagtgaa
     1201 gctgacgcag ttgcccccgc cggggaagta gaagtcctcg gccagcggca aggacacgcg
     1261 cgccatgatg tcctcgggtg ttgctccggg catggcggcc cagtcgaagg cggcgctgcc
     1321 ggagttgtcg ccgtcgacca ggcccttccg gcgccacagg gcgtcggg
//
LOCUS       DAOSPL010001453         1368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_96974_length_1368_cov_2.813404, whole genome shotgun sequence.
ACCESSION   DAOSPL010001453 DAOSPL010000000
VERSION     DAOSPL010001453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96974_length_1368_cov_2.813404"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1368
                     /locus_tag="PLQ54_20590"
     CDS             <1..>1368
                     /locus_tag="PLQ54_20590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOS95718.1"
                     /translation="RSLAVHATLLALVLVSASLYTYHTTRARLVSELRLRLEVACALA
                     EDSLVRQPPMQGELPGRVIELAALCSARVEAFSPPGVLEAASEETLSSADQRLEPEVA
                     SARSQGHGVAERRVVPHHPARLFVARYRPGGRWLVRLSAPLDSIADAALNSAWKTLLA
                     GTPSAIALVGLLAWGQRVWARSTGLVRDRIAAMGAAGETTRRDTAIPPELRPLVEACA
                     DSAQRLGQSQRELGDAVSRLTTILDQMADGVLLLSPDQSIQVINPAAAAMLGTDRRVA
                     TGRPLVEATLDFDLAELVRRALGTGVMVEDRPRLSDDGARVISAAAKPLIGPAGERAG
                     VIITLRDVTQAHRFQEARQDFVANASHELRTPLAAISAMAETLQDGAADDPGARARFL
                     ARIIDTTRRMTQLVDDMMALAQLEESSSPGPRGRLLDVRAALEDAASRLEPQAAARGI
                     SITIDT"
BASE COUNT          193 a          498 c          458 g          219 t
ORIGIN      
        1 ccgatcattg gctgtccacg caacactgct cgccttggtg ctcgtctcag ccagcctcta
       61 cacctaccac accacgcggg ctcgcctggt atcggaactc cggctgcggc tggaggtggc
      121 ctgcgctctt gcggaggact cgctggtccg gcagcctccc atgcaggggg aactgccggg
      181 gcgcgtcatc gagctggcag cgctctgctc ggcacgcgtc gaggccttct caccgcccgg
      241 ggtgctcgag gccgcgtcgg aggagacgct ctcgagcgcc gatcagcgtc tggaacccga
      301 ggtagccagc gcgcggtcgc agggccacgg ggtcgccgag cgacgtgtgg ttccacatca
      361 ccctgcgcgc ctgttcgtcg cccgctatcg cccgggcggc cgttggcttg tacggctctc
      421 ggcccccctg gactctatcg cggatgccgc gctgaactcg gcgtggaaga ccctcttggc
      481 cggcactccc tccgccattg ctctggtggg actgctggcg tggggtcagc gcgtgtgggc
      541 gcggtccacc ggactggttc gggaccgcat cgcggccatg ggggctgccg gcgagaccac
      601 aagacgggac accgccatcc cgcccgaact ccggccgctc gtcgaggcgt gtgccgactc
      661 agcccagcgc cttgggcagt ctcagcgcga gctgggtgac gccgtctcgc gcctcacaac
      721 catcctggac cagatggccg acggcgtgct ccttctcagc cctgatcaga gcatccaggt
      781 gatcaacccc gctgcggccg ccatgcttgg taccgatcga cgcgtggcga ccggccgccc
      841 gctggtggaa gctaccctgg acttcgacct cgccgagctc gttcgccgcg ccctcggcac
      901 gggtgtcatg gtcgaggacc ggccgcgcct gtccgatgac ggcgcccggg tcatctcagc
      961 ggcggccaag ccgctgatcg gaccggccgg cgagcgcgcc ggtgtgatca tcacgctccg
     1021 cgacgtgacg caggcgcacc ggttccagga ggcccgccag gacttcgtcg ccaacgccag
     1081 ccacgagttg cgtactccat tggccgccat ctcggccatg gccgagaccc ttcaggacgg
     1141 cgcggcggac gaccccggtg cccgcgccag attcctcgct cgcatcatcg acaccacgcg
     1201 gcgcatgacc cagctcgtag acgacatgat ggcattggcc cagttggagg agagcagctc
     1261 cccaggcccc cggggacggc tcctggatgt gcgggcggcg ctggaggatg cggcgtctcg
     1321 actcgagcct caagccgctg cgcgaggtat ctcgatcacg atagacac
//
LOCUS       DAOSPL010001454         1368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_96983_length_1368_cov_2.632140, whole genome shotgun sequence.
ACCESSION   DAOSPL010001454 DAOSPL010000000
VERSION     DAOSPL010001454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96983_length_1368_cov_2.632140"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1123)
                     /locus_tag="PLQ54_20595"
     CDS             complement(23..1123)
                     /locus_tag="PLQ54_20595"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS95719.1"
                     /translation="MSKDTQASSTPSSVTRREFLKTSTAAAVAGAMATQLLTRAYAQE
                     NNVLKIGLIGCGGRGTGAAANALEADPNTQLVAVGDAFQERLESSLANLKDTEFGDRV
                     LVDPDHQFVGLDAYQKVIDSGVDVVLLTTVPHFRPMHMEAAIAAGKHVFAEKPVAVDP
                     VGVRKVLALSEEAKQKGLAVVSGLCWRYELGMRDVVKRVQDGQIGDIIAVQSTRFSGS
                     VGTPTPRQPGWGDFRFQLKNWYYHTWLSGDFNVEQFVHELDKVAWAVGSYPTSCLCTG
                     GRQVRTGPEFGDIFDHFDAIYEWPNGIRYFAANRHWDGCSGIHRDYVMGTQGAADLMA
                     YTITGANPWKGEGNLGPAYVREHREMFESTTK"
BASE COUNT          213 a          456 c          441 g          258 t
ORIGIN      
        1 ggaagctcgt gattccgatt cactacttcg tcgtgctttc gaacatctcg cggtgctcgc
       61 gcacgtaggc ggggccgagg ttgccctcgc ccttccacgg gttggccccg gttatggtat
      121 aggccatgag gtcggccgcg ccctgggtgc ccatgacgta gtcgcggtgg atgccggagc
      181 acccgtccca gtggcgattg gcggcaaagt acctgatacc gttgggccac tcatatatgg
      241 cgtcgaagtg gtcgaagatg tcgccgaact cgggcccggt gcgcacctgt ctgccgccgg
      301 tgcacagaca cgacgtagga tagcttccga cggcccaggc caccttatcg agttcatgca
      361 caaactgctc gacgttgaag tcgccgctga gccaggtgtg gtagtaccag ttcttcagct
      421 ggaagcgaaa gtcaccccac ccgggctgac gtggggtggg tgtgcccacg ctccccgaga
      481 agcgggtgct ctggaccgcg atgatgtcgc cgatctggcc atcctgcacc cgcttcacga
      541 catcgcgcat gcccagctcg tagcgccagc acagaccgga tacgacggcc aggcccttct
      601 gcttcgcctc ctcgctcagt gccagcacct tgcgcacgcc taccggatcg acggccaccg
      661 gcttctcggc gaacacgtgc ttgcctgccg cgatggcagc ctccatgtgc atggggcgga
      721 agtgcggcac ggtcgtcagc aggacgacat cgactccgct gtcgatgacc ttctgatagg
      781 catccagccc cacgaactgg tggtcggggt ccaccaggac ccggtcacca aactcggtgt
      841 ccttgaggtt cgccagactg ctctcaagcc gctcctggaa tgcatctccc acggcgacga
      901 gctgcgtatt cgggtcggcc tccagagcgt tcgccgcggc accggtgccc cggcctccgc
      961 aaccgataag cccgatcttg agaacgttgt tctcctgggc atacgcacgg gtgagaagct
     1021 gtgtcgccat ggcgccggca accgctgcgg cggtcgacgt cttgagaaac tcgcggcgcg
     1081 tgacggagct cggcgtcgag ctcgcctgcg tatccttgct cattgaggag cctccaagcg
     1141 tcggaccggg gtccgagtgt gtgatggtac ggatggtccg gccagtccgt ggccgccggc
     1201 acccggctgg tgcgggtgac tgctgctggc cacagagcct tttcccccct tctcgcccgg
     1261 ctccttcccc aaggaccaag ttgggggtgg agccgcgggt caggccgccg ccccattggc
     1321 ggtagcttag ggctcccgtg gcccatccgc ctggttgcgg aggggcgc
//
LOCUS       DAOSPL010001455         1364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_97387_length_1364_cov_3.632544, whole genome shotgun sequence.
ACCESSION   DAOSPL010001455 DAOSPL010000000
VERSION     DAOSPL010001455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97387_length_1364_cov_3.632544"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1364)
                     /locus_tag="PLQ54_20600"
     CDS             complement(<1..>1364)
                     /locus_tag="PLQ54_20600"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOS95720.1"
                     /translation="TNPSARPLDLAASLVLGRDLMPELKQSEALHLAPGESRTLSIEA
                     ADVSTKRFELVARVGADGGPAFLDRTVSWPATGPWVWSVAKAVVPPVDFQFAYYPYGN
                     TMRILADVSNLPADAVLGELTGVVRTKTGEVVYTFGFDALKNGRQELAFGLPPLEGEY
                     EIALKATGENVPADEVVRPFRRTRFEWEHTPLGRSTTVYPPFEPIRVDGHDVRTVLRK
                     HVMGDSGLWDQVEAKGAPLLAAPMRWEARVGGEHAPVTAGPLTFAEVADNQAVARTSI
                     TAGALQGDVGCTWDYDGLMRVDLTLAPSAGQSVDGLQLVIPVDQSQATHLHAMGDGIR
                     NTLYERVPEGEGVVWTAERVQANDIPPRFCSYIYVGTPVRGLCWFAENDRGWGWDPST
                     PNLELVRQDGQVQLRVHLINRPLVIDAPRTITFGLLAAPVKPRLAPDWRHKFRRDGYS
                     ILGT"
BASE COUNT          231 a          496 c          425 g          212 t
ORIGIN      
        1 ccgtccccag aatcgagtag ccgtcccgcc gaaacttgtg ccgccagtcc ggcgcgagtc
       61 gcggcttcac cggagccgcc agcagcccga aggtgatggt tcgcggcgca tcgatcacca
      121 gtggccggtt gatcagatgc acccgaagct gcacctggcc atcctgccgc accagctcga
      181 gattgggcgt cgacgggtcc cagccccacc ctcggtcgtt ctcggcgaac cagcacaggc
      241 cacggacggg cgtgcccaca tagatatagg agcagaaacg cggcgggatg tcgttggcct
      301 gaaccctttc ggctgtccag acgacgccct ccccctccgg caccctctca tacaaggtgt
      361 tgcggatgcc atcgcccatg gcgtgcagat gcgttgcctg cgactggtct acgggtatga
      421 ccagttggag cccatcgact gattgtccag cggacggtgc aagtgtcagg tcgacccgca
      481 tcagcccgtc gtagtcccac gtgcacccaa cgtcgccctg gagcgccccc gcggtgatgg
      541 aggtccgcgc cacggcctgg ttgtcggcca cctcggcaaa ggtaagtggc cccgccgtta
      601 ccggcgcgtg ctccccaccg accctcgcct cccaccgcat cggggcggcc aggaggggtg
      661 cgcccttggc ctccacctgg tcccataggc ccgagtcgcc catcacgtgc ttccggagca
      721 ccgtccgcac atcgtgcccg tcgacgcgga tcggctcgaa gggcggatag accgtggtgc
      781 tccgcccgag cggtgtgtgt tcccactcga agcgcgtccg ccggaacggc cggaccacct
      841 cgtcggcggg aacgttctca cccgtagcct tcagcgcgat ctcatactcg ccctcgagtg
      901 gcggcagccc aaaagcgagc tcctgccggc cgttcttgag cgcatcgaaa ccaaaggtat
      961 atacaacttc cccggtcttc gtccggacca cgccggtgag ctcgcccagc accgcatcgg
     1021 ccggcaggtt gctgacatcg gccagaatgc gcatggtgtt gccgtaggga tagtaggcga
     1081 actggaagtc gaccggcggc accacggcct tcgccaccga ccatacccac ggacccgtgg
     1141 ccggccagct caccgtgcgg tccagaaagg ccggcccgcc gtcggcgccg acacgagcga
     1201 cgagctcaaa gcgcttcgtc gacacatccg ccgcctcgat cgagagcgtc cgcgactcgc
     1261 cgggcgccag atgcagcgcc tcggactgct tcagctcggg catcaagtcc cgcccgagaa
     1321 ccagcgaggc agccagatcc agcggccgtg cggaggggtt cgtg
//
LOCUS       DAOSPL010001456         1363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_97476_length_1363_cov_3.262997, whole genome shotgun sequence.
ACCESSION   DAOSPL010001456 DAOSPL010000000
VERSION     DAOSPL010001456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97476_length_1363_cov_3.262997"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="PLQ54_20605"
     CDS             complement(<1..1317)
                     /locus_tag="PLQ54_20605"
                     /inference="COORDINATES: protein motif:HMM:NF024794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M60 family metallopeptidase"
                     /protein_id="HOS95721.1"
                     /translation="MTGRNLRADLGGNQVLAAAGLMLADGMLGETGPKGYLADRVGLE
                     LAHAGRALEALEDHAAGRAALSQEDLNQVSTTLGTAARAVPPGDTLFLPRIKALCESP
                     AARCVPAPDVPVTLDQPLARIAATLLAAELAEARPEAVAAHPSAEAFPGAVPADAPRV
                     RRVLDVDTAVPDWHSTGLYAAPGEVIDVTVPESAAGRGLQVRIGCHTDEIWHLDAWKR
                     FPEITRAFAMDHPTTRAANAFGGLVYIVVPRGCALGHIEVAIEGAVEAPLYVYGRTGR
                     SAWRDTLRHAPGPWAELATDRVVITVPSSVIRELDDPEALLGFWNHVMDCCADLAAIP
                     RERERPERYVADVQISAGYMHSGYPIMTHLDAAPLSVDVAKLKSLGSWGHFHEMGHNH
                     QSGHWTFDGTGEVTVNLFSLYVSERACHLQAPAQPAVAPTSAIINWV"
BASE COUNT          203 a          485 c          483 g          192 t
ORIGIN      
        1 cacccagttg atgatggcgg aggtcggcgc cacggccggc tgggccggcg cctgcagatg
       61 gcacgcgcgc tccgagacgt acagcgagaa caagttcacg gtcacctcac cggtaccatc
      121 gaaggtccag tgccccgatt ggtggttgtg gcccatttcg tggaagtgcc cccacgaacc
      181 gaggctcttg agcttcgcca cgtcgaccga caggggcgcc gcgtctaggt gagtcatgat
      241 ggggtagccc gagtgcatgt acccggcgct gatctgtacg tcggccacgt accgctccgg
      301 gcgctcccgc tcgcgcggga tggccgccag atcggcgcag cagtccatca catgattcca
      361 gaagccgagc agggcttcgg ggtcatcgag ttcgcggatg accgatgacg gcaccgtgat
      421 gaccacgcgg tcggtggcca actcggccca cgggcccggg gcgtggcgga gggtgtcgcg
      481 ccacgccgac cggccggtgc ggccgtacac atacagcggc gcctcgacgg ctccctcgat
      541 ggcgacctcg atgtggccga gagcacagcc tcgcggaacc acgatgtaga ccagcccgcc
      601 gaaggcgttc gcggcccgcg tggtcggatg atccatggcg aatgcgcggg tgatctccgg
      661 gaaccgcttc caggcgtcga ggtgccagat ctcgtcggtg tggcagccga tgcggacctg
      721 tagtccccgt cccgccgcgc tctcggggac ggtcacgtcg atgacctcgc ccggagcggc
      781 atagagaccg gtgctatgcc agtcgggcac cgcggtgtcg acgtccagga cccgccgcac
      841 ccggggggcg tcggccggga cggcgcccgg gaaggcctcg gccgacgggt gtgccgcaac
      901 cgcctctggg cgcgcctccg ccagctcggc cgccaggaga gtcgccgcga tgcgtgccag
      961 tggctggtcc agcgtgaccg ggacatcggg agccgggacg caccgagccg cgggcgactc
     1021 gcacaacgcc ttgatccggg ggaggaacag cgtgtcaccc ggcggcacgg cccgagccgc
     1081 ggtgccgagg gtggtggaga cctggttcag gtcctcctgg ctcagcgcgg cccgaccggc
     1141 cgcgtggtct tcgagcgctt ccagcgcccg gcccgcgtgc gcgagctcga gacccacccg
     1201 atcggcgagg tagcccttcg gcccggtctc tccgagcatg ccatcggcca gcatgagccc
     1261 cgccgccgcc agcacctggt tcccgccgag gtcggcacgc aggttcctgc cggtcacctg
     1321 ctcccaaccc cacggacaga cgccggtgat gaaccctccg ccc
//
LOCUS       DAOSPL010001457         1362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_97593_length_1362_cov_2.664116, whole genome shotgun sequence.
ACCESSION   DAOSPL010001457 DAOSPL010000000
VERSION     DAOSPL010001457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97593_length_1362_cov_2.664116"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /gene="fabG"
                     /locus_tag="PLQ54_20610"
     CDS             <1..652
                     /gene="fabG"
                     /locus_tag="PLQ54_20610"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOS95722.1"
                     /translation="ALNDIAHTPATDETLAALSAAGAETGVFIADVRDETAVRAMVDE
                     IRATLGPVDILVNNAGVLRDTFISFMREQDWDDVVDTSLKGAFLCTKAVVRDMARRKW
                     GRIVNLASDAGRMGDAMRANYSAAKAGMMGLTRACARELARQGITVNAVAPGIVETPM
                     IADLKEQRLEQYRSLVPLGRFGRPDEVAAVIAFLASGEASYITGCTLSVDGGMCMS"
     gene            687..>1362
                     /locus_tag="PLQ54_20615"
     CDS             687..>1362
                     /locus_tag="PLQ54_20615"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOS95723.1"
                     /translation="MQLRRVGITGMGVVSCLGVGVEAFGEALFAGRSGLSVIDRFDPA
                     GLRNGLMGVVRDVPPLPDWAPGGTSSRDVVYAFSAADEALRSAGLVPPLSEPRTGCVL
                     STNFAGAEAMTRFAECPEDGSAFAACRFDTVVRGLASALSMRGPAFCVSVSCASGTAA
                     IGLGSDLVRTGRARRVLAGGYDVLALLTQAGLSCIRTNSTDTVRPFDVNRSQTIFGEG
                     AAMVVLE"
BASE COUNT          181 a          383 c          544 g          254 t
ORIGIN      
        1 cgcgctgaac gacattgcgc acactccggc aactgacgag acgctggcgg ctctgtcggc
       61 tgctggcgcg gagaccggcg tgttcattgc ggacgtgcgg gacgagaccg ccgtgagggc
      121 catggttgat gagatccggg cgacgctggg gcccgtggac attctggtaa acaatgcggg
      181 agtgctccgt gacacgttca tttcgttcat gcgagagcag gattgggacg acgtggtgga
      241 cacgtcgctg aagggcgcct tcttgtgcac gaaggccgtg gtgagggaca tggcccggcg
      301 gaagtggggg cgcatcgtta acctggcatc cgacgcgggg cggatgggcg acgccatgcg
      361 ggccaactac tcggcggcca aggcgggcat gatgggcctc acgcgggcgt gtgcccgcga
      421 gcttgcgcgg caggggatca ccgtgaatgc ggtggctccc ggcatcgtgg agacgccgat
      481 gatcgcggac ctgaaggagc agcggctcga gcagtaccgc tcgctggtgc ctctggggcg
      541 gtttgggcgt ccggacgagg tggcggcggt gatcgccttc ctggcgtcgg gggaggccag
      601 ctacatcact gggtgcacgc tgtcggtcga tggcggaatg tgcatgtcct gagccgctct
      661 cggcggcgat ggcgaacggg ggcggaatgc agcttcggcg tgtgggcatc acgggtatgg
      721 gcgtggtgag ttgccttggg gtgggcgtgg aggcgttcgg cgaggccctc tttgcgggga
      781 ggtccggact gtccgtgatc gatcgcttcg atcccgcggg gctgcggaac ggcttgatgg
      841 gggtggttcg cgacgtccct cccctacccg actgggctcc tggagggacg tcgtcgcggg
      901 acgtggtcta tgcgttttcg gcggcggatg aggcgcttcg atcggcgggc ctggtcccgc
      961 cgttgtcgga gccgcggacg ggctgtgtgc tgtccaccaa ctttgctggg gctgaggcca
     1021 tgacgcgttt cgccgaatgc ccggaggacg gttcagcctt tgcggcgtgc cggttcgaca
     1081 cggtggtgag gggtctcgcg tcggcgctat cgatgcgggg cccggcgttc tgcgtgtcgg
     1141 tctcatgcgc gtccggcacg gccgccatcg ggctcgggag cgacctggtg cgaacgggcc
     1201 gggcccggcg ggtgctggca ggtgggtacg atgtgctcgc ccttctcacc caggctgggc
     1261 tgtcgtgcat ccggacgaac tcgaccgaca cggtgcggcc ctttgatgtg aaccgcagcc
     1321 agacgatctt cggcgaaggc gcggcgatgg ttgttctcga gg
//
LOCUS       DAOSPL010001458         1361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_97703_length_1361_cov_2.462481, whole genome shotgun sequence.
ACCESSION   DAOSPL010001458 DAOSPL010000000
VERSION     DAOSPL010001458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97703_length_1361_cov_2.462481"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PLQ54_20620"
     CDS             complement(<1..965)
                     /locus_tag="PLQ54_20620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95724.1"
                     /translation="MAFLRIPPLAVGATLLLLLEPSAGQALPLRIGFVDPALPGVTPS
                     RNAAALAFAGSVGEAVRLRFAVGKGWLDAAGAPHAPEEFDVIWYHQGDSTTDAGLDRE
                     SCADLGEYVDGGGSLLLTGAAGQVLNTAGIEPTGLRVLGPTAASYTSGLLVPPEHRGH
                     PAFAGLDATQPIILTSVGGNALADFYGTAGPHGTLLAEGNAGLGERPLVEYEVGAGHV
                     VFVGWRLPDFTTANDAFRPNLERLTANLLHYLADTNTNRGRLVRPPGQSTYERFLGMP
                     FFRGTAPGDLALGGARPGEWAEVLTAEGEGAIAAGHGLFVREAEAT"
     gene            complement(1155..1230)
                     /locus_tag="PLQ54_20625"
     tRNA            complement(1155..1230)
                     /locus_tag="PLQ54_20625"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1195..1197),aa:Val,seq:gac)
BASE COUNT          213 a          464 c          485 g          199 t
ORIGIN      
        1 gttgcctctg cctcgcggac gaatagcccg tgtccggccg ctatggcccc ctccccctcg
       61 gcagtcagca cttcagccca ttcgcccgga cgcgcccctc cgagggccag gtctcccggc
      121 gccgtcccgc ggaagaacgg catgcccagg aatcgttcat aggtggattg gccgggcggg
      181 cgcaccagac ggccgcggtt tgtgttggtg tccgcgaggt agtgcagcag gttcgcggtg
      241 aggcgctcga ggttgggccg gaacgcatcg ttggctgtgg tgaagtccgg gaggcgccag
      301 ccgacgaaca ccacgtgtcc ggcgccaacc tcatactcga ccagcgggcg ctcaccgagg
      361 ccggcattgc cctcggcgag gagagtcccg tgtggacccg ccgtgccgta gaaatcagcg
      421 agcgcgttgc cgcccaccga ggtgaggatg atcggctgtg tcgcgtcgag cccggcgaag
      481 gccggatgac cccgatgctc tggcggcacc agcagtcccg acgtatagga cgccgccgtc
      541 gggcccagca cgcggaggcc ggtcggctcg ataccggccg tgttcagcac ctggcccgcc
      601 gcaccggtca gcaacaggct tccgccgcca tcgacgtact cccccagatc ggcgcaggac
      661 tcccggtcga gcccggcgtc ggtggtggag tcgccctggt ggtaccagat gacatcgaac
      721 tcctccggcg cgtgtggggc gccggcagcg tcaagccatc ctttgcccac tgcgaagcgc
      781 aggcggacgg cctcgcccac cgacccggcg aaggccagtg cagcggcgtt acgggagggc
      841 gtcactccag gcagcgcggg gtcgacaaac ccgatgcgca gaggcagtgc ctgaccggcg
      901 ctcggttcga gcaggagcag caacgtcgcg cccacggcca acgggggaat cctcaggaat
      961 gccatggccc gacctccgca gtcttccctc agcgccgcgg gtgttcgccg gctcgcgacc
     1021 gggggcctcg cggtgcccgg atctggggag gatgtggcgg tgggctggcc gcgcgacgga
     1081 tcgtggatgg ctggcctggt aacgccagag ccctcgatcg cgcgaccgag ggcccttaag
     1141 gctaccgcag cagatggtgg gcggtgtagg aatcgaacct acgacctctt ccgcgtcaag
     1201 gaagcattct agcccctgaa ctaaccgccc ctgtgacgcg gtgattctag cagacgccgc
     1261 cggcccatgt aaagacggac gcctggcgtg acacggggcg gatgtggcgg gccagcccgg
     1321 tggctcactg cccgggtggc gcgaggaagg cggccttgct g
//
LOCUS       DAOSPL010001459         1359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_97860_length_1359_cov_4.129601, whole genome shotgun sequence.
ACCESSION   DAOSPL010001459 DAOSPL010000000
VERSION     DAOSPL010001459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97860_length_1359_cov_4.129601"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..>1359
                     /locus_tag="PLQ54_20630"
     CDS             162..>1359
                     /locus_tag="PLQ54_20630"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOS95725.1"
                     /translation="MERVRKCPCLWSHRPRRRWLVAVSVAALALSLSSLSPAAADEDA
                     PTYESLMDEAQGCKAKGDFQGMADAIGRALELGEGTEYAWRSLAWSLARSGHFERSFD
                     IAKGNIERFGETAWPLAQLADSALYVHDPDVTYEALSKAKQLPEDELGEAGSALKDAK
                     TRWLGMFGTRRISVTWTIDPKEQGISTPDHILWVRLPQLRHPRQDVTYEIVSGARDGE
                     LVQSEDIDFLKVKYDPETPIVIRTEAVLHCCTVPLERLRETDLEGIPADISEEFLAAS
                     TDVPIDPQGQTCQELIRELKGETPYETVMNIMRWFRDNFTYKENPTQDSEGLLNEHFG
                     VCHHYSVAISALCRAAGIPARIVGGEVFGWANEDDQSVVGGGSHGWVEVYLNPIGWVE
                     LDGLGYD"
BASE COUNT          240 a          428 c          473 g          218 t
ORIGIN      
        1 cggacccggg cgtgctcggg cttgggcccg cccgagctgc gtgcgcgctt cactcctggc
       61 ccccattccg agtcggaggg ctgtgggccg cggcgacgaa ccaaagagga ggccgcgcga
      121 gcagacccag gccattcgat caaggaggat gtgccgtgca gatggagcgt gtccgcaagt
      181 gtccctgtct ctggagccat cgcccccggc ggcggtggct cgtggccgtc agtgtcgccg
      241 ccctcgctct atcgctctct tctctctcgc ccgccgctgc cgacgaagac gccccgacct
      301 acgaatcgct catggatgag gcccagggct gcaaggccaa gggggacttc cagggcatgg
      361 ccgatgccat aggccgggcg ctggaactgg gagaaggcac ggagtacgcc tggcgtagcc
      421 tggcctggtc gctggcgcgg tcggggcact tcgagcggtc cttcgatatc gccaagggaa
      481 acatcgagcg cttcggagag acggcgtggc cgctggcgca gctcgccgac tcggcgctgt
      541 atgtgcacga tccggacgtg acctacgagg ccctgtccaa agcgaagcag ctccccgagg
      601 acgagctcgg cgaggccggc agcgctctca aggatgccaa gacgcgctgg ctcgggatgt
      661 tcgggacgcg gcgcatatcg gtgacatgga ccatcgaccc caaggagcag ggcatttcga
      721 cgccggacca catcctgtgg gtcaggctgc cgcaactccg tcacccgcga caggacgtga
      781 cgtacgagat tgtcagtggc gcccgggacg gcgagctcgt gcagtccgaa gacattgact
      841 tcctgaaggt gaagtacgac cccgagacgc ccatcgtcat ccgcaccgag gcggtactgc
      901 actgctgcac ggttccgctg gagaggctcc gcgagacaga cctcgagggc atccctgcgg
      961 acatctccga ggagtttctg gccgcgtcga cggatgtgcc catcgatcct cagggccaga
     1021 cgtgccagga gctcatcagg gaactcaagg gcgagacgcc ctatgagacc gtcatgaaca
     1081 tcatgcgctg gttccgcgac aacttcacgt acaaggagaa tccgacgcag gattccgagg
     1141 ggctactgaa cgagcacttc ggtgtctgcc accactatag cgtggccatc agcgcgctgt
     1201 gccgggcggc gggcatcccg gcgcggatcg ttggcggcga agtgttcggc tgggccaacg
     1261 aggacgacca gtcggtggtg ggtgggggct cgcacggttg ggtcgaggtg tacctgaatc
     1321 ccatcggctg ggtcgagctg gacgggctgg gctacgaca
//
LOCUS       DAOSPL010001460         1356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_98200_length_1356_cov_3.147579, whole genome shotgun sequence.
ACCESSION   DAOSPL010001460 DAOSPL010000000
VERSION     DAOSPL010001460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98200_length_1356_cov_3.147579"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLQ54_20635"
     CDS             complement(<1..186)
                     /locus_tag="PLQ54_20635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95726.1"
                     /translation="MSSVRPSASYCFCPGRRHRLCGRLGRCAGFLAFVILGARAALFP
                     VYGVHTPCGWIDEARWRV"
     gene            526..>1356
                     /locus_tag="PLQ54_20640"
     CDS             526..>1356
                     /locus_tag="PLQ54_20640"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95727.1"
                     /translation="MTTRWILTAAALAAGTATAEAGSFTLVRDGRPAATIVLSTQPSR
                     AAEFAARELQAHVRMMTGVELPIVSEGATVEGARILVGESAATRRLGLRGDSFASQEY
                     LVGYRDHDLILIGRDAPTAPVPAKWVEAPLGKCLEFDGQDDVLVFPEPGFSDEAGTLE
                     ARVWLPAEAPKAHGTILRLDGADPWTYHIVQRDAGTSVISYTVYDGTNGAAVVSRPLE
                     EGWHHVAATHSRATGLIELFIDGVSQGTAPFGITNCATAPLHIGGYQSATVGNAFAGR
                     I"
BASE COUNT          221 a          435 c          500 g          200 t
ORIGIN      
        1 caccctccag cgtgcttcgt cgatccaccc gcagggcgtg tggacaccat agacgggaaa
       61 gagcgcggca cgggcgccaa ggatcacgaa ggccagaaac ccggcgcacc gccccaagcg
      121 gccgcagagc cggtgcctcc ggccagggca gaagcagtac gaggccgacg ggcggacgga
      181 gctcattctg gccacctccc ataagccatt ggtaccggct tcggcgcgcg ttggtggggg
      241 aaggaggggc gcggcggcgg accgggccga cggcgaggtg cggcgcgctg gctgcggcat
      301 gcatggctgt cctgtcgctg ttcacggctc gtgctccttg cctgcccagc ggcctgcttc
      361 gtattgacta ggacggtctc gcgacgcgaa ggggttgcag tggccacgcg atggcgccag
      421 ctctgatgcg tgcgactgct ccgtacgtta cagccacggg ctggcggagg ctgatacact
      481 acggaggcac accgtgcttc acgaatggcg gggggacttg gagcgatgac gacccgatgg
      541 atactcacgg ctgccgcgct ggcagcgggc accgccacag ccgaagcggg gtcgttcacg
      601 ctggtgcgcg acgggaggcc ggccgccacc atcgtgctct cgacccagcc gagtcgcgcc
      661 gccgagttcg ccgcccggga gctgcaagcc cacgtccgaa tgatgaccgg ggtcgagctg
      721 ccgatcgtct ccgagggcgc gacggtcgag ggagcgcgga tcctggtggg cgaaagcgcg
      781 gcgacacgca ggctcggcct ccgtggagac agcttcgcgt cgcaggagta tctggtgggc
      841 taccgggacc atgatctgat actcatcggg cgcgacgcgc cgacggcgcc ggtaccggcg
      901 aagtgggtgg aggcgccgct aggtaagtgt ttggagttcg acggccagga tgacgtgctc
      961 gtcttcccgg agccgggctt ctcggatgag gctgggacgc tggaggcccg ggtgtggctg
     1021 ccggcggagg cgccgaaggc gcacggcacg atcctccgtc tggatggcgc agacccgtgg
     1081 acgtatcaca tcgtccagcg cgacgcgggc accagcgtca tctcctacac ggtatacgat
     1141 gggacgaacg gcgctgcggt ggtctcgagg ccgctggagg agggttggca ccacgtcgcc
     1201 gccacgcaca gccgggcaac gggactcatc gagctgttca tcgacggcgt gagccagggg
     1261 accgctccgt tcggcatcac caactgcgcc acggcgcctc tgcatatcgg cggctatcag
     1321 agcgcaactg tcggcaacgc cttcgcgggc cgtatc
//
LOCUS       DAOSPL010001461         1353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_98508_length_1353_cov_4.184900, whole genome shotgun sequence.
ACCESSION   DAOSPL010001461 DAOSPL010000000
VERSION     DAOSPL010001461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98508_length_1353_cov_4.184900"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="PLQ54_20645"
     CDS             complement(<1..1291)
                     /locus_tag="PLQ54_20645"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOS95728.1"
                     /translation="MLHQCIAFGATALAIAAAGHAQEAVPRPEHPRPDFERAGWLNLN
                     GQWQFEIDNELTGDERGLATGHDLEGTITVPFCPESRLSGVAHTDFMKQVWYRREFRV
                     PREMTGQRLLLHFGAVDWHARVWVNGTLVGEHKGGYTPFACEITNQVRSGDNEVVVCA
                     RDDTRSGLQATGKQSHERDSYGCVYTRTTGIWQTVWLEAVGTTYLSDFCLVPDSDTGR
                     VTFQGTVNGPCEGVKLRLKALAGPRTVAHDEVPARGRSTLALLKVDDPHLWQPGKPYL
                     YDLEIVLEKDGHDIDRVRSYFGFRKVHVDGFRMLINDKPVFQRLVLDQGFYPDGIYTA
                     PSDEALRADIELSMAAGFNGARLHEKVFEPRLLYWADKLGYIVWGEYPDWGVDHDSAA
                     AMGQVAMEWREVLHRDRNHPSIVGWCPLNETGGGEGNA"
BASE COUNT          221 a          464 c          428 g          240 t
ORIGIN      
        1 aggcgttgcc ttcaccacca cccgtctcgt tgagcgggca ccagcccacg atggacgggt
       61 ggttgcggtc ccgatgcagc acctctcgcc actccatagc gacctggccc atggctgcgg
      121 cactgtcgtg gtcgacgccc cagtctggat actcgcccca cacgatgtag cccagcttgt
      181 cggcccagta gagcaaccgc ggttcgaaaa ccttctcatg cagccgcgcc ccgttgaacc
      241 ccgcagccat cgaaagctcg atgtcggccc gcagagcctc atcgctcggc gccgtgtaga
      301 tgccatcggg gtagaacccc tggtccagca ccagtcgctg gaacaccggc ttgtcgttga
      361 tgagcatgcg gaacccgtcg acatgcacct tgcggaaacc gaagtagctc cgcacccggt
      421 cgatgtcatg cccgtccttc tcgagcacga tctccaggtc gtagagatac ggctttcccg
      481 gctgccacag gtggggatca tcgaccttga gcagtgccag cgtcgatcgt ccgcgagcgg
      541 gcacctcatc gtgggcgacg gtgcgcgggc cggcgagcgc cttcagccgc agcttcacgc
      601 cctcgcacgg gccgttcact gtaccctgga aggtcactcg ccccgtgtcc gagtccggca
      661 caaggcagaa gtctgacaag tacgtggttc cgacggcctc gagccacacc gtctgccaga
      721 tgcctgtggt tcgcgtgtag acacacccgt agctatcgcg ctcgtgcgac tgcttccccg
      781 tggcttggag gcccgaccgg gtgtcatcgc gggcgcagac gacaacctcg ttgtcgccgg
      841 aacgcacttg gttggtgatc tcgcacgcga atggcgtgta cccgcccttg tgctcgccga
      901 cgagagtgcc gttcacccac acgcgcgcgt gccagtcgac cgcgccgaag tgcagcaaca
      961 gacgctggcc ggtcatctcg cggggcacgc ggaactcgcg gcggtaccac acctgcttca
     1021 tgaaatccgt gtgtgcgacg ccggagagtc ggctctccgg gcagaagggg accgtgatcg
     1081 tgccctccag gtcgtgcccg gtggccaggc cgcgctcgtc gccggtaagc tcgttgtcga
     1141 tctcgaactg ccactggccg ttcaggttga gccatccggc gcgctcgaaa tcgggccgag
     1201 ggtgctccgg ccgcggcacg gcctcctggg catggccggc ggcggcgatg gcgagcgccg
     1261 tggcgccaaa ggcgatgcac tgatgcagca tgggacgcaa cctccagata gtcgcacgtc
     1321 tcgctcttag tggctctctc gtgattgggc agt
//
LOCUS       DAOSPL010001462         1353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_98514_length_1353_cov_3.470724, whole genome shotgun sequence.
ACCESSION   DAOSPL010001462 DAOSPL010000000
VERSION     DAOSPL010001462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98514_length_1353_cov_3.470724"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            319..>1353
                     /locus_tag="PLQ54_20650"
     CDS             319..>1353
                     /locus_tag="PLQ54_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific methyltransferase EcoRI family
                     protein"
                     /protein_id="HOS95729.1"
                     /translation="MPSKSAGSGSKQSSLSGAKAAKVDEFYTQLSDIEKELRHYKKHF
                     KGMVVYLNCDDPRESEFFHYFSYNFEKLGLKKLIAACYKSQSMDLFSTEDSEEAVYLE
                     YTGDKDGDRIPGRDEIEVQAFEGDGDFRSPESIALLKQADIVVTNPPFSLFREYVEQL
                     MEHEKKFLIIGNMNALKYKEIWPYIKQDKLWLGVTRAGTGQMWFRVNEDFPVKSGQKV
                     GDDGYRYQTIGNSAWFTNLDHSKRHEDLILFKTYDPNEYPTYANFDGIEVSKVVNIPI
                     DYPGVMGVPITFLGKYNPDQFEILGTSLELAGPMSDVAPKGTYPQGGPNFYLSNNDGT
                     FRRTYERLAIR"
BASE COUNT          309 a          403 c          394 g          247 t
ORIGIN      
        1 cgggatgtct tgagacatca catgtgtgtc cggaggggga cttgaacccc cacgacatgg
       61 cctacgtccg tccttgtata ccagcagatt ccgtaccctg gagcggtgag cgcgcggccc
      121 gcgtgtcgga tacgtgtcgg atcgcagctg ctgaacgccg cgccagccga ggggcaggac
      181 gatttgaggc gggccccggg cgcggctcac ctaggctcgc cggactctcc cgtgtagcgc
      241 ctccgccgac cagtcgcccg tcgtggctat ggtgagcctg aacaggcggc acgacaggca
      301 gggatggtgg agggcaacat gccgagcaag agtgcgggca gcggatccaa acagagcagc
      361 cttagcggag ccaaagctgc caaggttgat gagttctaca cccaactgtc cgacatcgag
      421 aaggagcttc ggcactacaa gaagcacttc aagggcatgg tcgtctacct caactgcgac
      481 gacccccgcg agtcggagtt cttccactac ttcagctaca acttcgagaa gctcggcctg
      541 aagaagctca tcgccgcctg ctacaagagc cagagcatgg atctgttctc gactgaagac
      601 agcgaggagg ccgtctacct cgagtacacc ggcgacaagg acggcgaccg catcccaggt
      661 cgcgacgaga tcgaggttca ggccttcgag ggtgatggcg acttccgctc acccgagagc
      721 atcgcgttgc tcaagcaggc cgacatcgtt gttaccaacc ctccgttcag cctcttccgt
      781 gagtacgtcg agcagcttat ggagcatgag aagaagttcc tcatcatcgg aaacatgaac
      841 gccttgaagt acaaggagat ctggccgtac atcaagcagg acaagctctg gctgggtgtc
      901 acccgggccg gaactgggca gatgtggttc cgtgtgaatg aggacttccc tgtgaagtca
      961 gggcagaaag tcggtgatga tgggtaccgc tatcaaacca tcgggaactc cgcttggttc
     1021 acaaaccttg accacagcaa gcggcacgag gacctcatcc tcttcaaaac gtacgacccg
     1081 aacgagtatc ccacctacgc gaacttcgac gggatcgaag tgtccaaagt tgtcaacata
     1141 ccaatcgatt acccgggtgt catgggtgta ccgatcacct tcttgggcaa gtacaaccct
     1201 gaccagttcg agattctggg gaccagcctc gagctggccg gccccatgtc cgatgttgca
     1261 ccgaagggca cctatcccca aggtgggccc aacttctatc tcagcaataa cgacggaacc
     1321 ttccgacgaa cctacgagcg tcttgcgatc agg
//
LOCUS       DAOSPL010001463         1352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_98653_length_1352_cov_2.555127, whole genome shotgun sequence.
ACCESSION   DAOSPL010001463 DAOSPL010000000
VERSION     DAOSPL010001463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98653_length_1352_cov_2.555127"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PLQ54_20655"
     CDS             <1..568
                     /locus_tag="PLQ54_20655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95730.1"
                     /translation="APVVKFQNLDAFGGPPVTLENRSAARTMVVESCGVRTVGAGGGD
                     IFATDCPSLVELRKPGQHMWARQLNPEGTDDVGLVRNAGAGLWALGVKCEGKGVRFRT
                     SDGGRTEILGAFIYGPGVEPNDPRPMFDIDDGAMSIMGLREIAFDVPTFAIKVRERRE
                     AETRTLGVDREPGWIGWSMFSGWAAPPG"
     gene            688..>1352
                     /locus_tag="PLQ54_20660"
     CDS             688..>1352
                     /locus_tag="PLQ54_20660"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOS95731.1"
                     /translation="MATPAPCVSAITALIFTTASALAQKGPIVKSEFIYDEAPFPSCH
                     ASTLVETEPGRLLCAWFGGTDEGEKDVAIWLSEWDGAAWSAPREVARDPKEPCWNPVL
                     FHTRAGETLLFYKAGPSPQTWSGLMVRSADRGVTWGAPEWLPAGILGPIKNKPIELDD
                     GSIVCGSSVESYGLWGCWAEITPDAGHTWAKHGPINVPGQLNGIIQPAMLDLGEGRIR
                     ILCR"
BASE COUNT          218 a          444 c          469 g          221 t
ORIGIN      
        1 ggcgccggta gtgaagttcc agaacctcga cgcgttcggt ggacctccgg tgactctcga
       61 gaatcgctcc gccgctcgca cgatggtcgt cgagagctgt ggcgtccgta ccgtcggcgc
      121 cggcggaggc gacatcttcg ccaccgactg cccgtcgctc gtcgagttgc ggaaacccgg
      181 tcagcacatg tgggcgcgcc agctcaaccc ggagggcacc gacgatgtcg gactcgtccg
      241 caacgccggc gccggtctgt gggcactggg cgtcaagtgc gagggcaagg gggtccgctt
      301 ccggacgagc gatggcggac gcacggagat cctcggcgcg ttcatctacg gcccgggtgt
      361 cgagccgaat gacccgcgcc cgatgttcga tatcgacgac ggcgccatgt cgatcatggg
      421 gttgcgcgag atcgcgttcg acgtgccgac cttcgcgatc aaggtacgcg agcgccgcga
      481 ggctgagact aggaccctcg gggtagaccg ggagcccggg tggatcggct ggtcgatgtt
      541 ctcgggctgg gcggcaccac cgggttaggg cgtcggtgtg aacgcgccgg ccagctcctc
      601 ctggaggccg atcggccgct cgcctggaag gatatgaagg cgccgcgccc acgcgagggc
      661 ggccagacag gatgtaggag cacgaacatg gccaccccag ctccgtgtgt cagcgccatc
      721 acggcgctca ttttcaccac cgcgtcggct ctcgcccaga agggtcccat cgtgaagagc
      781 gagttcatct acgacgaagc gcccttcccg tcgtgtcatg cgtcgacact ggtggagacc
      841 gagcccggcc ggctgctctg tgcctggttc ggcggcaccg atgagggcga gaaggatgtc
      901 gccatctggc tctccgagtg ggacggcgct gcctggtcgg ccccccgcga ggtggcccgc
      961 gacccgaagg agccctgctg gaaccccgtg ctgttccaca cacgggcggg cgagacgctg
     1021 ctcttctaca aggcagggcc gtctccgcag acgtggagcg gcctgatggt ccgctctgcg
     1081 gaccgtggtg tcacctgggg cgctcccgaa tggcttccgg ccggcatact tgggcccatc
     1141 aagaacaagc ccatcgagct cgacgatggt agcatcgtat gcggctcatc ggtcgagagc
     1201 tacggcctct ggggctgctg ggccgagatc acaccggacg ccggccacac gtgggccaaa
     1261 catggcccta tcaacgttcc cggccagctc aacggtatca tccagccggc gatgctcgat
     1321 ctgggtgaag ggcgcatccg catcctgtgc cg
//
LOCUS       DAOSPL010001464         1351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_98745_length_1351_cov_3.038580, whole genome shotgun sequence.
ACCESSION   DAOSPL010001464 DAOSPL010000000
VERSION     DAOSPL010001464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98745_length_1351_cov_3.038580"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="PLQ54_20665"
     CDS             complement(<1..1321)
                     /locus_tag="PLQ54_20665"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOS95732.1"
                     /translation="MRLDLPIPREVARYLMGMGKRGGHRTGDVHWKWDVERADNMVWI
                     GDVDAGVQLKLMENVDVWHTVSLRETGIPESWGNGGKGGCDVTEEGDTVLVRAYSGSR
                     TMAAGESVRFRFRLLVTPFRRIDPKHWNWRYGDTDGPATVLHVHHGTVQNPYINYPFW
                     TWESLRDIVTDVKSKRTRGTDFGKLTYPAAGNIDMRQGHMHLWTTIGFDPHAGGPGDA
                     RFNQPLFSVDFPNGDAAGLYWNIDDRGMRTYVRRGPSINNQYPVLFGTHQPGWEKGQR
                     HLITLSWGKRLAVYVDGELAGEAHYQGLVDEPLGGVRLRFEGHFGLEGILISSVPFGG
                     GAVGAPTAGPDTLLLDTFSDWQGDDQTLPARGIGGRAEGVVQRSDGPFGKTVTFSQRE
                     IEGPEKGVNIYYTVGQISNHVAELWPLRSLGDEVFLTRELLAFKVGDT"
BASE COUNT          218 a          480 c          410 g          243 t
ORIGIN      
        1 cggtgtcgcc caccttgaac gccagcagct cgcgggtcag gaacacctcg tcgcccagac
       61 tgcgcaatgg ccacagctcg gccacgtggt tactgatctg gccgacggtg tagtagatgt
      121 tcacgccctt ctcaggaccc tcgatctcgc gttgagagaa ggtgactgtc ttcccgaacg
      181 gtccgtcgga ccgttggacg acgccctcgg cccggccgcc gatcccgcgc gcagggagcg
      241 tctggtcgtc cccctgccag tcgctgaacg tgtccaggag tagtgtgtcg ggccctgcgg
      301 tcggcgcccc gaccgccccg ccgccgaacg gcacactcga gatgagaatc ccctccagcc
      361 cgaagtggcc ctcgaaccgg aggcgcacgc cacccagcgg ctcgtccacc aggccctggt
      421 aatgtgcctc gccggccagc tccccgtcga catacacggc caggcgcttg ccccacgaca
      481 gggtgatcag gtgccgctgc cccttctccc atcccggctg atgcgtcccg aacaggaccg
      541 ggtactggtt gttgatcgac gggccccggc gcacgtaggt ccgcatgccg cggtcgtcga
      601 tgttccaata gaggcccgcc gcatcgccgt tcgggaagtc caccgagaac agtggctggt
      661 tgaagcgtgc atcgcccggc ccacccgcgt gggggtcgaa cccgatggtc gtccacaggt
      721 gcatatggcc ctgccgcata tcgatgttcc cggcggctgg gtaggtcagt ttgccgaagt
      781 cggtgccgcg ggtgcgcttc gacttcacgt ccgtcacgat gtcccgcaga ctctcccaag
      841 tccagaacgg atagttgatg taggggttct ggactgtgcc atggtgcacg tgcagcaccg
      901 tcgccggtcc gtcggtgtcc ccgtagcgcc agttccagtg tttcggatcg atgcgccgaa
      961 agggggtcac cagcagccgg aaccgaaacc ggacgctctc gccagcagcc atggtgcgag
     1021 acccggagta ggcacggacg agaaccgtgt cgccctcctc ggtgacgtcg catccgccct
     1081 tcccgccgtt cccccatgat tcagggatac cggtctcccg caggctcacc gtgtgccaga
     1141 catcgacatt ctccatgagc ttgagctgga cgcccgcgtc aacgtcaccg atccagacca
     1201 tgttgtccgc acgctcgaca tcccacttcc agtgcacatc gcccgtgcgg tgcccgcctc
     1261 gcttccccat gcccatcagg taccgggcta cttccctggg aatgggtagg tcgagcctca
     1321 catcggagat ctcctgatcg cggacggcct t
//
LOCUS       DAOSPL010001465         1350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_98850_length_1350_cov_2.946718, whole genome shotgun sequence.
ACCESSION   DAOSPL010001465 DAOSPL010000000
VERSION     DAOSPL010001465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98850_length_1350_cov_2.946718"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLQ54_20670"
     CDS             <1..189
                     /locus_tag="PLQ54_20670"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOS95733.1"
                     /translation="GAGPDVDGQILVLYDMLGLGAGKGFRHVKQYAALDEIIGGAAGA
                     YVDDVRRGAFPPSEGAGS"
     gene            213..1049
                     /gene="panC"
                     /locus_tag="PLQ54_20675"
     CDS             213..1049
                     /gene="panC"
                     /locus_tag="PLQ54_20675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOS95734.1"
                     /translation="MGALRSWLEAHRVAGRRIGLVPTMGYLHAGHASLIRRARQECDI
                     VVVSVFVNPTQFGPGEDYDRYPRDLEHDQALCEAEGADAIFAPPVVEMYPTGAPCTRV
                     TVARVSEGLCGRSRPHHFAGVATVVTKLLNIVQPHRAYFGEKDYQQLAVIRTMVADLN
                     LPVDIVGCPTVRESDGLAMSSRNSYLSPELRARAPAIYRALCAGRDAAQAGERSAGAI
                     AARVKAALEPVPDAAVEYVEVVDAESLAPLQEVGGGAVIAVAVRLGGTRLIDNIAIKP
                     VP"
BASE COUNT          197 a          462 c          488 g          203 t
ORIGIN      
        1 ggtgccggtc cggatgtcga cgggcagatc ctggtgctct acgacatgct gggcttgggt
       61 gccggcaagg gcttccggca tgtgaagcag tacgcggccc tcgacgagat catcggcggc
      121 gcggcgggcg cctatgtcga cgacgtgcga cgcggtgcgt tcccgccgtc cgagggggcc
      181 ggctcgtgag ccccgccacc gtcttcgagc gcatcggcgc actccgctcg tggctcgagg
      241 ctcatcgcgt tgcgggccgc cgcatcggcc tcgtccccac catgggatac ctccacgccg
      301 ggcatgcctc cctcatccgc cgcgcccggc aggagtgcga catcgtagtg gtctccgtgt
      361 tcgtcaaccc cacccagttc gggccggggg aggactacga ccgctacccg cgcgatctcg
      421 agcacgatca agcactgtgc gaggccgagg gcgccgacgc catcttcgct ccccccgtgg
      481 tcgagatgta ccccaccggc gctccctgca cgcgcgtgac ggtggcccgg gtgagcgagg
      541 gcctgtgtgg acggtcgcgg ccccaccact tcgccggcgt ggccacggtg gtcacgaagc
      601 tgctcaacat cgttcagccc caccgggcct acttcggcga gaaggactat cagcagcttg
      661 cggtcatcag gacgatggtg gcggacctca acctgccggt cgacatcgtc ggctgcccca
      721 cggtccgcga gtccgacggc ctggccatga gctcccgcaa cagctacctg agccccgagt
      781 tgcgcgcgcg ggcgccggcg atctaccggg ccctgtgcgc cggccgcgat gccgctcagg
      841 ccggcgaacg gtcggcgggg gccatcgccg cgcgcgtgaa ggcggccctg gagccggtcc
      901 ccgatgcggc ggtcgaatac gtcgaggtcg tcgacgcgga gtcgctggcc ccactccagg
      961 aagtcggcgg gggggcggtg atcgctgtgg ccgtgcgcct gggggggacc cgcctgatcg
     1021 acaacatcgc catcaagcct gtcccgtgag ggccgtgggc accaaatgcc ccgctgccgg
     1081 caggagtggc gtaccagcca cccgaagaat ggcaaaagga cgtgggccgc ggccgcgggc
     1141 ggcggcccgc ttgacgttca gctcacagaa tcgagagaca cccgcaaccg aacgggcgtc
     1201 ttcgatagtt gagaccaggg tgtaccggca taggggagcg tgttccgcac ccgatggggc
     1261 agcgcaaggc ggtgtgggtc gcgatggcgg gggtcgcgct atcctttgcg ggagcgggac
     1321 ccggcttcgg ttgggacctg agtcaggatg
//
LOCUS       DAOSPL010001466         1348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_99119_length_1348_cov_2.095901, whole genome shotgun sequence.
ACCESSION   DAOSPL010001466 DAOSPL010000000
VERSION     DAOSPL010001466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99119_length_1348_cov_2.095901"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1348)
                     /locus_tag="PLQ54_20680"
     CDS             complement(<1..>1348)
                     /locus_tag="PLQ54_20680"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOS95735.1"
                     /translation="VNEHRVGQTGAFPPTYEGLSGIPRAYAVPSAIVHAGALNLIAVR
                     VYDGGGAGGLVAGPVRLECGRGYARLEGPWLCRVGDDAAWAALEPRDRPLLERNAFEE
                     AIVIPRFVGEATRPASDLALWYRRPASKWVEALPVGNGRLGAMVFGGVPEERIQFNED
                     TLWTGQPHDYTHPGAAQHLPEIRRLLAEGKQAEAEELAMREFMSIPLGQKAYQPFGDL
                     VVRFPSVAHVTDYRRELDLDSAVATVTYRAEGVNYTREVFASHPDQAIVMHLAADQPG
                     RVTFGAGLTSPHGSAAAHMVDERTIALAGRVEEGGVRFEARLLASADGGSVAATAKGL
                     QVDGANSVTLILVAATSYSSFRDISADPAERCEDALSDARQHSYASLRQRHIADHREL
                     FRRVSLNLGRTPSADRPTDERLQTVADAPDPQLAALFFQYGRYLLIASSRPGTQPAN"
BASE COUNT          213 a          471 c          468 g          196 t
ORIGIN      
        1 tgttcgccgg ctgagttccc gggcggctgc tggcgatcag cagatagcgc ccatactgga
       61 agaacagcgc ggccaactgc gggtccggcg cgtcggccac agtctgcaga cgctcgtcag
      121 tgggccggtc cgcggagggt gtgcgaccga ggttgagcga cacccggcgg aacagctcgc
      181 gatggtcggc gatgtggcgc tgccggagag aggcgtagct gtgttggcgt gcgtccgaca
      241 gcgcgtcctc gcatcgctcg gcggggtcag cactgatatc gcggaagctc gagtagctgg
      301 tggcggccac caggatgagc gtcaccgagt tcgccccgtc gacctgcaat cccttggcgg
      361 tcgccgcgac ggagcccccg tcggcggacg ccagaagacg cgcctcgaac cggacgccgc
      421 cctcctcgac ccgaccagcc agggcgatcg tgcgttcatc gaccatatgg gcagcggcag
      481 agccgtgcgg gctggtcagc cccgccccga aggtcacccg cccgggttgg tcggcggcga
      541 ggtgcatgac gatggcctgg tcgggatggc tcgcgaacac ctcccgggtg tagttgacgc
      601 cctcggcgcg gtaggtgacg gtcgccaccg ccgaatcgag gtccagctca cgccggtagt
      661 cggtgacatg ggcgacactg ggaaaccgca caaccaggtc gccgaacggc tggtaggcct
      721 tctggcccag cgggatactc atgaactccc gcatggcgag ttcctcggcc tcggcctgct
      781 tgccctcggc gagcaggcgg cggatctccg gcaggtgctg ggccgctccg gggtgggtgt
      841 agtcgtgtgg ctgccccgtc cagagcgtgt cctcgttgaa ctggatccgc tcctcgggca
      901 cgccaccgaa caccatggcg ccgagacggc cgttgccgac cggcagggcc tcgacccact
      961 tcgacgccgg ccggcggtac cacaaagcga ggtcactggc gggtcgagtc gcctcaccga
     1021 cgaaccgggg gatcacgatg gcctcctcaa acgcgttccg ctccagcaat ggcctatctc
     1081 ggggctcaag cgccgcccac gcagcgtcgt caccgacgcg gcacagccag gggccttcga
     1141 gccgggcata cccgcgtccg cactcgaggc gcaccggccc cgccacgagg ccccctgcgc
     1201 ccccgccatc atagacgcgc accgcgatca ggttgagcgc acccgcgtgg acgatagccg
     1261 acggcacggc atacgctcgt gggatgcccg aaaggccctc ataggtgggc ggaaatgcac
     1321 ccgtctgccc gacccgatgc tcgttgac
//
LOCUS       DAOSPL010001467         1347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_99163_length_1347_cov_3.894737, whole genome shotgun sequence.
ACCESSION   DAOSPL010001467 DAOSPL010000000
VERSION     DAOSPL010001467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99163_length_1347_cov_3.894737"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..1207
                     /locus_tag="PLQ54_20685"
     CDS             71..1207
                     /locus_tag="PLQ54_20685"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOS95736.1"
                     /translation="MDVARARADMPACQERIYLDHAGVAPLSGPAAEVVAAAAADMAR
                     NGWLNRKRWGRLVDRGRVLFARMIGAAPGDIAYVKNTSQGLLLAANSLPLGEGDNVVV
                     FHGEFPANVYPWLALARRGVETRLVRPEPDRRIPPGLVASVMDSRTRAVALSAVQFGS
                     GFRADLGAIGDLCRARGAYFVVDMIQCVGAVPMDVSETPFDVGAADGHKWLLGPEGIG
                     YLYCRPEILDQLAPTYMGWNSVRDAYDFEHYDATPRPDARRFEEGSLNVLGACGLAAS
                     VELLASWGRDWVYARVLELTHHVAEQVRRRGFELLSPMERLEERSGIVIFRVPGHDSG
                     ALVESLVSGGVLCAARGGGIRFSPHAYNTEDEIDEAFRRLDGQV"
BASE COUNT          187 a          399 c          503 g          258 t
ORIGIN      
        1 ccgttgacga cccgacctac cgggaggaga atgcgatccc gcacccatcg ccgtgccgag
       61 aggtgagagc atggacgtcg cgagagcccg agccgacatg cccgcctgcc aggagcgcat
      121 ctacctcgac cacgcgggtg tagcgccctt gagcgggccg gcggccgaag tcgttgccgc
      181 ggccgcggcc gacatggctc gcaacgggtg gctgaatcgc aaacgctggg gccggctcgt
      241 cgaccggggc cgggtgctgt tcgcccggat gatcggcgcg gcacccggtg acattgcgta
      301 cgtgaagaac acatcgcagg gcttgctctt ggccgcgaac agccttccgc taggcgaggg
      361 tgacaacgtg gtcgtgttcc atggcgagtt tccggccaac gtgtatccgt ggctggcgct
      421 ggcgcgccgg ggggtagaga cacggctagt tcgtccggag cctgaccggc gcataccgcc
      481 cggcttggtc gcgtcggtga tggactctcg cacacgggcc gtggccctga gcgccgttca
      541 gttcggctcc gggttccggg cggatctcgg tgccatcggc gacttgtgtc gcgcgcgcgg
      601 cgcctacttc gtggtggaca tgatccagtg tgtcggtgcc gtgcccatgg atgtgtcgga
      661 gacccctttt gatgtcggtg cggctgacgg acacaagtgg ctgttggggc ccgagggcat
      721 cgggtacctg tactgcaggc cggagatcct ggaccagttg gcgccgacct atatgggatg
      781 gaacagcgtt cgggatgcat atgacttcga gcactacgac gccactcctc gaccggatgc
      841 ccggcgcttt gaggagggct cgctcaatgt tctgggcgcc tgcgggctag cggcatcggt
      901 ggagctactc gcgagttggg ggcgcgactg ggtgtacgcc cgggttctcg agctgacgca
      961 ccatgtcgcc gagcaggtgc gtcgccgggg ttttgagctg ctgtcaccga tggagcggct
     1021 ggaggagcgt tcgggcattg tgatctttcg cgtgcccggc cacgactcgg gagcgttggt
     1081 ggagtcgctg gtgtccggcg gggtgctgtg tgcggctcgg ggcgggggca tccgcttctc
     1141 gccgcacgcc tacaacaccg aggacgagat cgatgaggct ttccggcggt tggatgggca
     1201 ggtgtagtcg atgtcgagca tcgtcccgcg cttctttgtg cgctatgcgg gccacgtgac
     1261 cttcgaactg ggcgcgccga cgggcgaggt gttgctcgtc gacccgttct ttggtgacgc
     1321 gttcccgtgg gagggccatg tggagcg
//
LOCUS       DAOSPL010001468         1345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_99423_length_1345_cov_2.791473, whole genome shotgun sequence.
ACCESSION   DAOSPL010001468 DAOSPL010000000
VERSION     DAOSPL010001468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99423_length_1345_cov_2.791473"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1345
                     /locus_tag="PLQ54_20690"
     CDS             <1..>1345
                     /locus_tag="PLQ54_20690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95737.1"
                     /translation="LLRGLDGQLSYERYGSNDGFTVCHWHPAVQAANRETRRQFTEEL
                     PVDVLFQDQCGARSWRYDTNPASPTPYAYIDGLLSMVDEDSQVVPLSTESGWDHVAQF
                     ESQLCGMSWGLVPTEFAPEWRVLMKNQVPPDTWEVFPLAQHIAHDKTAMVHHDLGQFV
                     TNRETLTWTLALGFCMSARVSATALASDPALQWLRWLDRVQKSICARYLGEPVLAFAH
                     DRGPNPTAQDDGAIDATYGNVRIRANLGPATREVSGVWLPPFGFDATGPGLHAGELPE
                     GDTTSPVAYVAEFGQDGGDLWLRAPGQVRATLRLPFDASGRCRVTLDEAAPIEAQVAD
                     GVLTVETPPVTPPGTEPIPPEIANLPRSQWPGPKPAIGVIDLGPGAHLVWTRITPAEW
                     VDALSDSRLVRKHGLQVRRLANVDDLLSALSDTEHRWFAIVNPYGEAFPVPAKDR"
BASE COUNT          217 a          515 c          399 g          214 t
ORIGIN      
        1 cgctgctccg gggcctggat ggccagctct cctacgaacg ctatggctcc aacgacggct
       61 tcaccgtctg tcactggcac cccgccgtgc aggcagccaa ccgagagacc cgccggcagt
      121 tcaccgagga gctgccggtc gatgtgctgt tccaggacca gtgcggcgcc cgcagctggc
      181 gctacgacac gaacccggcc tcccccacgc cctacgccta catcgacggt cttctgtcga
      241 tggtcgatga ggatagtcag gtggtaccgc tatccaccga gagcggatgg gaccacgtcg
      301 ctcagttcga gagccagctc tgcgggatga gttgggggct ggtgccgacc gagttcgcgc
      361 ccgagtggcg cgtgctcatg aagaaccagg tgcctccaga cacctgggaa gtctttcccc
      421 tggcccagca tatcgctcat gacaagaccg cgatggtcca ccacgatctc ggccagttcg
      481 tgaccaaccg cgagacgctc acttggacgc tcgcacttgg attctgcatg agcgcccgcg
      541 tcagcgccac cgccctggcg tccgatcctg ccctccagtg gctccggtgg ctcgaccgtg
      601 tccagaagtc catctgcgcc cgctacctgg gcgagcccgt actggccttt gcgcacgacc
      661 gcggtcccaa cccgaccgcc caggacgatg gcgccatcga cgccacctac ggtaatgtcc
      721 gcatccgcgc caacttgggc cctgccaccc gcgaggtcag cggcgtctgg ctgcccccct
      781 tcggtttcga tgccactggc cccgggctcc atgccggcga actccccgaa ggcgacacga
      841 cctcccccgt ggcatacgtg gccgagttcg gccaggacgg cggcgacctc tggctccgcg
      901 cccccggcca agtccgggcc actctccgcc tgcccttcga cgcctccgga cgctgccgcg
      961 tgaccctcga cgaagccgcg ccaatcgaag cccaagtggc cgacggagtc ctcaccgtcg
     1021 agacaccgcc cgtcacgccc ccggggactg aacccatccc gccagagatc gcgaatctgc
     1081 cccgaagcca gtggccggga ccgaagccgg ccatcggcgt catcgatctc gggcccggtg
     1141 cccacctcgt ctggacccgc atcaccccgg cggagtgggt cgacgcactg agcgactccc
     1201 gactcgtccg caaacacggc ctccaggtgc gtcgtctggc caatgtggac gacctcctct
     1261 ccgccttgtc tgacaccgaa caccggtggt tcgccatcgt caatccatac ggcgaggcgt
     1321 tcccggtgcc cgccaaggat cggtg
//
LOCUS       DAOSPL010001469         1341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_99864_length_1341_cov_2.590980, whole genome shotgun sequence.
ACCESSION   DAOSPL010001469 DAOSPL010000000
VERSION     DAOSPL010001469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99864_length_1341_cov_2.590980"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PLQ54_20695"
     CDS             complement(<1..718)
                     /locus_tag="PLQ54_20695"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOS95738.1"
                     /translation="MGPTAEKALVLSVAAGKGGTGKTLLATSLAIVAHDRGPRPVTLI
                     DCDVEEPNAHLLLHPAWEDQELVTVGVPEVDADRCISCGRCAAACHTGAIALIRDRVL
                     VFPDLCSSCGVCSYVCPVSAITEKAHSVGLVATGRSAEGIRIVSGMLDVGRARTGPVI
                     DAARAKCLPDHVTIIDAPPGNACSMQDSVENSDVCLLVTEPTPFGCSDLALAIETCRE
                     MNVPCAVVVNRDGLGSARVED"
     gene            complement(766..1275)
                     /locus_tag="PLQ54_20700"
     CDS             complement(766..1275)
                     /locus_tag="PLQ54_20700"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOS95739.1"
                     /translation="MRAAIASSGPTLDSAVDGRFGRCRCFVVVDTETLEYTVIENAAA
                     SRGSGAGIMAAQLVANSGAEAVVAGNLGPNAYQALSASGMALYQAWGGTVREVAQALQ
                     RGELTPFAGASVAAHAGMAASPPPPGDTDSLEQLRQRVNGLTADLKSAQAKLDRLQGQ
                     APGASEGDR"
BASE COUNT          205 a          493 c          428 g          215 t
ORIGIN      
        1 cgtcctcgac ccgcgcgcta ccgagcccgt ccctgttcac gactaccgcg cacgggacgt
       61 tcatctcgcg gcatgtctcg atggccaacg ccaggtcact gcacccgaag ggcgttggct
      121 ccgtgacgag tagacagaca tccgagttct cgaccgagtc ctgcatcgag cacgcattcc
      181 ccgggggcgc atcgatgatg gtcacgtggt ccggtaagca cttcgccctt gcggcatcga
      241 tcacggggcc agttctggcc cgcccgacat cgagcatgcc gctcacgatc cgaatgccct
      301 cggccgaccg ccccgtggca accagaccga ctgagtgggc cttctcggtg atggcgctca
      361 cgggacagac gtagctgcag acgccgcagc tcgagcacag gtctgggaac accagcaccc
      421 ggtcgcggat cagagcgatg gcgcccgtgt ggcacgcggc tgcacatcgg ccacatgaga
      481 tgcagcggtc ggcgtccacc tcggggacgc cgaccgtcac gagctcctgg tcctcccacg
      541 cggggtggag gagcaggtgc gcgttgggct cctcgacgtc acaatcgatc agcgtcaccg
      601 gccggggccc tcggtcatgc gcaacgatgg cgagactcgt cgccaggagc gtcttgccgg
      661 tgccgccctt gcctgccgcg acgcttagca ccagtgcctt ctcggctgtg ggacccaaac
      721 tcacaccttc atccacgacc cgcatcgccg gttcggccgg cgctcctacc gatcgccctc
      781 ggacgcgccg ggcgcctggc cctgcaggcg gtcaagcttg gcctgtgcgg acttgaggtc
      841 agccgtcagc ccgttgacgc gttggcgaag ctgctccaag gagtccgtgt cacccggcgg
      901 cggaggcgat gccgccatgc ccgcgtgcgc agcgacactg gcgcccgcaa acggcgtcag
      961 ctcgccacgc tgtagggcct gtgccacctc tctgacggtc ccaccccagg cctggtagag
     1021 cgccatgccc gaagcggaca gcgcctggta ggcgttgggg cccaagttgc ccgccacaac
     1081 ggcttcggct cccgagttgg cgacgagctg agcggccatg atgccggcac ccgacccgcg
     1141 gctcgccgcg gcgttctcga ttaccgtgta ctcgagcgtt tccgtgtcga cgaccacgaa
     1201 gcagcggcac cgcccgaagc ggccgtccac cgccgagtcc agtgtcggcc ccgaggacgc
     1261 gatcgcagct ctcacggttc attgcccctc tccgccgccc acgtggtctg gcggcaacgg
     1321 cctctggcta ggcctcgttc t
//
LOCUS       DAOSPL010001470         1341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_99870_length_1341_cov_2.454899, whole genome shotgun sequence.
ACCESSION   DAOSPL010001470 DAOSPL010000000
VERSION     DAOSPL010001470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99870_length_1341_cov_2.454899"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PLQ54_20705"
     CDS             complement(<1..818)
                     /locus_tag="PLQ54_20705"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOS95740.1"
                     /translation="MDKCEAAVHRIVHRRAPREWHDGFPLGNGAVGAMLWGDGDPLCL
                     TLDKSDLWDLRSDDAYLEHPDHSYAGLRRLVSEGRFDEAFEVFEARQLRDNPLGPTKI
                     SIGRAELTLGSATSYECSLELATATVAGTLRTETGEHRLLAFVHRERNIVCLRVTSPP
                     AGARLSLTPLCEMSDSLAALGLPNPAVDDDGPVRILCQSIPEGPAYAVAWNPRGPDFL
                     LAIESAQTAEAARVRALATWQAAADAGIDRLYEEHPRGWQGFWGESAVCLPEER"
     gene            988..>1341
                     /locus_tag="PLQ54_20710"
     CDS             988..>1341
                     /locus_tag="PLQ54_20710"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS95741.1"
                     /translation="MALRVAFAGLKHDHVRTILRLALDDPGVELAGVADSDLTNREAI
                     AQAFRVPVDYISHEALLESAEFDALVVCDAFGRRGEAAIAALETGKHVFSDKPLCTRE
                     VELHRIADLAREKGLE"
BASE COUNT          193 a          477 c          469 g          202 t
ORIGIN      
        1 atgcgctcct cgggcagaca gacggccgac tcgccccaga acccctgcca gccgcggggg
       61 tgctcctcgt acagccggtc gatgcccgca tcggccgccg cctgccatgt ggcgagagct
      121 cggacccggg ccgcttcggc tgtctgggcg ctctcgatcg ccaggaggaa gtccggcccg
      181 cgtgggttcc acgccaccgc gtacgccgga ccctctggga tgctctgaca cagaatgcgc
      241 acggggccgt cgtcgtcgac cgcggggttg gggagcccga gagcggccag cgagtcgctc
      301 atctcgcaaa gcggcgtcag gctcagacgc gcgcctgccg gtggcgaggt cacgcgcagg
      361 cagacgatgt tccgctcacg gtggacgaag gccaggagcc ggtgctctcc tgtctcggtc
      421 cggagagtgc ccgcgacggt cgcggtggcc agctccaggc tgcactcgta ggaggtcgcc
      481 gagcccagcg tcagttccgc ccggccgatg ctgatcttcg tcggacccag cgggttgtcg
      541 cggagctggc gggcctcgaa gacctcgaag gcctcgtcga accgcccctc tgacaccaac
      601 cggcgcagcc cggcgtagct gtgatccggg tgctccaggt aggcatcatc gctacggaga
      661 tcccacaagt ctgacttgtc cagcgtgaga cacagaggat cgccgtcccc ccacagcatc
      721 gccccgacgg ccccgtttcc cagggggaag ccgtcgtgcc actcccgcgg tgccctccgg
      781 tgcacgatcc ggtggaccgc ggcctcgcac ttgtccatag tggttgctcc tgcctggtgc
      841 tggagccggg ctggtggtgt cttcgccacc gcgccgccgc ggcctgcagc catgcaggca
      901 cccggccgcg atggggcgaa ggcgtctgca cagggcgagg tcctgagccc gtcgaagggc
      961 cccgcccggg ccgcgagggg agccggtatg gcactccgcg tcgcgttcgc tgggctgaag
     1021 cacgaccatg tccgcacgat actccggctg gcgctggacg acccgggcgt cgagttagcg
     1081 ggcgtggccg acagcgacct caccaaccgg gaggccatcg ctcaggcctt ccgcgtgccc
     1141 gtcgactaca tcagccacga ggcgctgctg gagtcggccg agttcgatgc cctggtcgtg
     1201 tgcgacgcat tcggtcgccg cggtgaggcc gccatcgccg cgctggagac cgggaagcac
     1261 gtcttcagcg acaagccgct ctgcactcgc gaggtggagc ttcaccgcat tgccgacctc
     1321 gcccgggaga agggcctgga g
//
LOCUS       DAOSPL010001471         1339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_100159_length_1339_cov_1.619938, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001471 DAOSPL010000000
VERSION     DAOSPL010001471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100159_length_1339_cov_1.619938"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..720)
                     /locus_tag="PLQ54_20715"
     CDS             complement(34..720)
                     /locus_tag="PLQ54_20715"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOS95742.1"
                     /translation="MIGRCTTELNPDQRSRNDNISIAAEKLDGTVVGPGQEFSFNEAV
                     GERSSELGYGPARVIRDGRSEPGIAGGICQLSSTLYNAALLAGMEITERRPHSRPVPY
                     LPAGLDATVSYGAADLRWVNVRPSPVTVRSRVGVDSVSVWLEGQSAGPEVRLCTEVVE
                     LIAPRPAAPPDPTGRAKSPPLPGAIVVASAGAPGYRVRVWAETETPDGGRQRVLVSED
                     LYEPVNAVVN"
     gene            complement(900..>1339)
                     /locus_tag="PLQ54_20720"
     CDS             complement(900..>1339)
                     /locus_tag="PLQ54_20720"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95743.1"
                     /translation="AVISREYFRIDRNAPAVKGQKYSVTPIDGEIGVRQEVLVRVAVH
                     ALTDLEYMAFEDPVPSGFEISEESVDAYSWYYWYDRSEARDSKMVVFATRIPAGKTQT
                     FEYVLVPERPGSYRVMPTEAWSMYYPGLHAHGNSSSITVTGSK"
BASE COUNT          255 a          463 c          387 g          234 t
ORIGIN      
        1 ggcacggcgt cgctccgggt ggcgcaatgc gagctagttc acgactgcgt tgacaggttc
       61 gtacaggtct tctgagacca atacgcgttg gcgtccccca tcaggcgtct cggtttccgc
      121 ccagaccctc actcggtacc cgggcgcgcc ggcgctggcc acgacgatgg ccccaggcaa
      181 cggcggcgac ttggccctgc cggtgggatc ggggggcgcc gcaggccttg gagcaatcag
      241 ttcgacaacc tcggtgcaca gccgcacctc gggcccagcg ctctgccctt caagccaaac
      301 ggacacactg tccacgccca cccgggaccg cacagtcacc ggagacggac gcacattcac
      361 ccagcgaaga tccgccgccc cgtaggacac ggtggcatcc agccctgcag ggaggtatgg
      421 cacgggccgc gagtgtggac gccgctcggt gatctccata cccgcaagca gcgcggcatt
      481 atagagagtc gaagacagct ggcatatgcc gcccgcgatg ccgggctcgc tccggccgtc
      541 ccgaatgact ctggccggac catagccgag ctcgctgctg cgctcgccta cggcctcatt
      601 gaacgagaac tcctgccccg gcccgaccac agtcccatcg agcttctctg cagcgatcga
      661 tatgttatcg ttacggctcc gctggtcggg gttgagctca gttgtgcacc taccgatcac
      721 gcaagtgccg tttccggtag ccaagtccgc gctcgccccc gaccgaaagc gggcagcagg
      781 cgcaaacctc agtgcagcca tgacggcgac caccgtcacc gccatggcta tcacgactat
      841 gcagcgcgcg cgtcgcccac acggcacccg ctctcacctc cgaagaccat gactgtcggc
      901 tacttcgagc ctgttaccgt tatcgacgag ctgttcccat gggcatgtag cccagggtag
      961 tacatggacc atgcctcagt gggcatcact ctgtacgaac cgggcctttc cggaaccaga
     1021 acatactcga acgtctgggt cttgccggcc ggaatgcgag tcgcaaacac taccatcttg
     1081 ctgtcgcgag cctcgcttct gtcgtaccag tagtaccacg aataggcgtc cacagattcc
     1141 tcggatatct caaaccccga cggaactggg tcctcaaagg ccatgtactc caggtcagtc
     1201 agggcgtgca cggcaacacg cacaagcacc tcctggcgga cgccgatctc gccgtcgatg
     1261 ggggtcacag agtatttctg tcccttgacg gctggggcat tccgatcaat ccggaagtac
     1321 tcacggctta tgacggcgc
//
LOCUS       DAOSPL010001472         1338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_100191_length_1338_cov_2.990647, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001472 DAOSPL010000000
VERSION     DAOSPL010001472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100191_length_1338_cov_2.990647"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PLQ54_20725"
     CDS             complement(<1..696)
                     /locus_tag="PLQ54_20725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOS95744.1"
                     /translation="MPRSPRSPEDGGPPTVPARTKLLLQVRETSKGFEHRHCHLSDGA
                     WEPDRDWKPGSLGASGPGVYSTNMERLSGPPDLEWPAAWSKLVVAPPPTPVLSAQSLL
                     ADLLATVGAERHHCVVLEPEPPQFGRWPSEAQALGKLGVPLPCKPYVHQVEALDLVRS
                     GKHVVIATGTASGKSLCYQIPILSSIMGDPQARALYVAPTKALCTDQLESWGGMLSRE
                     PIDGWLVEGELRGQ"
     gene            complement(723..>1338)
                     /locus_tag="PLQ54_20730"
     CDS             complement(723..>1338)
                     /locus_tag="PLQ54_20730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOS95745.1"
                     /translation="AFGLGIDLPRIDWVVHFYMPPSLLDYYQEIGRGGRAMDAKAGER
                     CRCLVLYDPQDRALVEQLVWGSVASADKISQRFRQLILGRNGQHGLRGPHEVLYDDAR
                     KVLLLPFRPMQTQYTVRIGHMLALQEIGVLKRRSENLFRGGNVYAQFDVAREELTAED
                     EAQLYERQERRRQTVLSRLDGMQCFCEADCAEARWGMLDEVFGR"
BASE COUNT          217 a          480 c          417 g          224 t
ORIGIN      
        1 ttgacctctg agctccccct cgacgagcca cccgtcgatc ggttcgcggc tcaacatgcc
       61 gccccacgat tccagctggt cggtgcagag ggccttcgtc ggggccacgt acagagcccg
      121 cgcttggggg tcacccatga tgctcgacaa gatcgggatc tgatagcaca ggctcttccc
      181 gctggcggtc cctgtcgcga taaccacatg cttcccactg cggaccaggt cgagggcctc
      241 tacctggtgc acatacggct tgcatggaag agggaccccg agcttcccga gcgcttgggc
      301 ttcactgggc cagcggccga actgcggcgg ctcaggctcc agaaccacgc aatggtgcct
      361 ctccgctccc accgttgcca gcaggtcggc gagcaggctc tgtgccgaga gaaccggtgt
      421 cggcggcggg gcgaccacga gcttcgacca ggcggcaggc cactcgagat cgggtgggcc
      481 cgagagtcgc tccatgttcg tcgaatacac cccgggccca ctcgcaccca ggcttcccgg
      541 tttccagtcg cggtccggtt cccatgcgcc gtcagacaga tggcagtggc ggtgctcaaa
      601 ccccttgctg gtctcgcgca cctgcagcag gagcttggta cgggcgggca cggtgggcgg
      661 gccgccgtct tccggtgatc tcggcgatcg gggcattagg gtccacctcc tgccgtggca
      721 tgctaccgac cgaagacctc atccagcatg ccccaccgcg cctccgcgca gtcggcctca
      781 cagaagcact gcatcccatc gaggcggctg aggaccgtct ggcggcggcg ctcctgccgc
      841 tcgtagagct gcgcctcgtc ctcagccgtc agttcctcgc gagctacgtc gaactgcgcg
      901 tacacgttgc cgccacggaa caggttctcg gaccgccgct tgagcacgcc gatctcctgc
      961 agggccagca tgtgcccgat gcgcaccgtg tactgggtct gcatgggccg gaacggcagc
     1021 aggagcacct tgcgcgcgtc gtcgtacagg acctcgtgag gcccgcgcag gccgtgttgg
     1081 ccgttccgcc ccagaatgag ctgccggaaa cgctgggaga tcttgtccgc cgatgccaca
     1141 ctcccccaca caagctgctc caccagggcc cggtcctggg ggtcgtacag aaccagacaa
     1201 cggcacctct cgcctgcctt cgcatccatc gctcgcccgc cgcgcccgat ctcctggtag
     1261 tagtccagca ggctcggcgg catgtagaag tgaaccaccc agtcgatccg cggcaggtcg
     1321 atacccagcc caaaggcg
//
LOCUS       DAOSPL010001473         1338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_100209_length_1338_cov_2.578332, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001473 DAOSPL010000000
VERSION     DAOSPL010001473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100209_length_1338_cov_2.578332"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="PLQ54_20735"
     CDS             complement(<1..1182)
                     /locus_tag="PLQ54_20735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95746.1"
                     /translation="MLVLPVAVSLLLLGDGTSGLQDPAILRARAMALEARGRTDAALE
                     AWERVVDRSVATDAQRLEAVAAIRRLRPEPPESPDPTRGRPWRTLVLIYRHLDFSWSA
                     PDGQTLWVRATVSAGEERRIRDGFAAFARHVLRFTAGAVRIDADYVVVDEPLTHLSGD
                     SGGPFWPAPWDVAATTDRLMAGREYDSVFVYVKCREGEGTGVPLLHGGGTFGADYGPH
                     GAGWTDIPFYPEWLDASGEIELHEWLHQIDWMFANVLGYPDAIVPSSDEGRREGDDGG
                     DPDYRRSADEPDWMGFYRHIMRDHITRLMWSECGMHHAPRTPWADSWLHEWLAYGPVA
                     RPGEAGAEAEVPSPASSSEWKPIPWPSIRVNLPAAPPGEDALFRLATWVRPPTDCRAT
                     LE"
BASE COUNT          218 a          459 c          454 g          207 t
ORIGIN      
        1 ctcgagagtc gcgcggcagt ccgtgggcgg gcggacccag gtggccaggc gaaacagcgc
       61 gtcctccccc ggtggcgctg cggggaggtt cacccggatc gagggccatg ggatcggctt
      121 ccattcagag ctggatgcgg gcgatgggac ctctgcctct gcgcccgcct ccccgggccg
      181 ggcgactggg ccatacgcaa gccactcgtg aagccagctg tcggcccagg gcgttctggg
      241 cgcatggtgc atcccacact cggaccacat caggcgcgtg atgtggtcgc gcatgatgtg
      301 ccggtagaac cccatccaat cgggctcgtc cgcactccgc cgatagtcgg ggtcgccccc
      361 gtcgtcgcct tcgcggcgcc cctcgtcgga cgaaggaacg atggcgtcgg ggtagccgag
      421 cacattggcg aacatccagt cgatctgatg cagccattcg tgcagttcga tctcgcccga
      481 cgcatcgagc cactctgggt agaacgggat gtccgtccac cccgcgccat gtgggccata
      541 gtcggctccg aaagtgccac cgccatggag cagcgggacc ccagtgcctt cgccctctct
      601 gcacttgacg tagacgaaca cgctgtcgta ctcgcgccct gccatcagcc ggtcggtagt
      661 agcggcgaca tcccatggcg cgggccagaa tgggcccccg gagtcacccg agaggtgggt
      721 caacggctca tcgaccacca cgtagtccgc atcgatgcgc accgcacccg ccgtgaaacg
      781 gagcacgtgc cgggcgaatg cagcgaatcc gtcgcggatc cggcgctcct cgccagcgct
      841 gaccgtcgcg cggacccata gcgtctgccc gtccggggct gaccacgaga agtcgaggtg
      901 tcggtagatg aggacgagcg tgcgccacgg gcgaccgcgg gtcgggtccg ggctctcggg
      961 cggctctggt ctcaaccgcc ggatggccgc caccgcctcg agccgctgag catcggttgc
     1021 caccgagcga tcgacgacgc gctcccacgc ttccaatgcg gcatcggtgc ggcctcgggc
     1081 ctccagggcc atcgctctgg ccctcaggat tgcgggatcc tgcagacccg atgtaccgtc
     1141 accaagaagc agcagcgaga cagcgacagg caagaccaac acggggcaca ctccttgggt
     1201 cagtggggcg cgacgatggt ccgcaggcca gaggcccgca ttcgcagtcg tgcttgtggg
     1261 ccgttgggac ccagcgccat ggtggcctag tggtaagtcg cctggcccca gtgtgagggc
     1321 cgctgcggaa tctggtcg
//
LOCUS       DAOSPL010001474         1335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_100541_length_1335_cov_2.533594, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001474 DAOSPL010000000
VERSION     DAOSPL010001474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100541_length_1335_cov_2.533594"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1335
                     /locus_tag="PLQ54_20740"
     CDS             <1..>1335
                     /locus_tag="PLQ54_20740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95747.1"
                     /translation="GDDDIFYTRLRLDHTFGDPERYARTAAGPFDAPSPIDPARTLTY
                     EDPRLPFDREDHRGEIRVPTEDEVLAPLPEGTVGAGESTTGAAQTAVFASRHTDWRWG
                     WPVAADDGDNDPNTGDPTGGNWIEVRVQEFVGGAWTDVAGQTWDTFAEDPATGLLYAM
                     SPNGVKAIEIDPAAGLVRFNETRAQLTDPSTVPAANQRVLATYTARALRLTTSSFDEA
                     QPAAAIMPWGFDRTTDEPEVIHVPASVLDPSAQDPDALFDFIRPERLMLLFTRKTTQG
                     QHALYYQTLSVPLDPNDNTPYDQRDPTWMFDDDNDRDKGDAWFPYQYTDSGPMAVPLE
                     RNVHESRVAPVAMPYVWWTRSLQDPAGVYLSAFRDFLTDSGLSTVVGPTTVWLFYATA
                     APHTPVDPESGAPVFDPDEGRYAADAPGDLDIFYGALSPRTIPTYRQDVDLS"
BASE COUNT          232 a          490 c          415 g          198 t
ORIGIN      
        1 agggcgacga cgacatcttc tacacgcgcc tgcgcctcga ccacaccttc ggcgaccccg
       61 agcggtatgc gaggactgcc gcggggccat tcgacgcccc gagccccatc gacccggccc
      121 gcacgctcac ctatgaggac ccccggcttc ccttcgaccg cgaggaccac agaggagaga
      181 tccgcgtgcc caccgaggac gaagtgctcg cgcccctgcc cgaagggact gtcggggccg
      241 gcgaatccac caccggcgcc gcccagaccg ccgtgttcgc cagccgacac accgactggc
      301 gctggggctg gcccgtcgcc gccgacgatg gggacaacga cccgaacacc ggggacccca
      361 ccggcggcaa ctggatcgag gtccgcgtgc aggagttcgt gggaggtgcg tggaccgatg
      421 tcgccggtca gacctgggac acgttcgccg aggatccggc taccgggctg ctgtatgcca
      481 tgagcccgaa tggggtcaag gccatcgaga tcgaccccgc cgcgggcctg gtgcggttca
      541 acgagacccg ggcgcagctg acggacccct ccaccgttcc cgccgcgaac cagagagtgc
      601 tcgcgaccta cacggcgcgg gccctgcgtc tcaccacgag ctcgttcgac gaggcacagc
      661 cggcggcggc catcatgccc tggggcttcg accgcaccac cgacgagccc gaagtgatcc
      721 acgtacccgc cagcgtcctg gatccgtccg cccaggatcc cgatgccttg ttcgacttca
      781 ttcgacctga gcggctcatg ctgctcttca cgcggaagac cactcagggc cagcatgctc
      841 tgtactatca gacactatct gtgccgctcg acccgaacga caacacgccc tatgaccagc
      901 gcgatccaac ctggatgttc gacgatgaca acgaccgcga caagggcgat gcgtggttcc
      961 cgtaccagta caccgactcg ggtcccatgg cggtgccgct cgagcggaac gttcacgagt
     1021 cccgagtggc ccccgtcgcc atgccctatg tgtggtggac acggagcctg caggacccgg
     1081 ccggcgtcta cctgtcggca ttccgggact tcctgaccga tagtggcttg agtaccgtgg
     1141 tgggcccgac gaccgtctgg ctgttctacg ccaccgcagc gccccacacg ccagtggatc
     1201 cggagagcgg cgctcccgtc ttcgatcccg acgagggcag gtacgccgcc gatgcgcccg
     1261 gagacctgga catcttctac ggcgcgctgt cacccaggac cattccgacc taccggcagg
     1321 atgtggatct ctcgg
//
LOCUS       DAOSPL010001475         1333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_100776_length_1333_cov_2.430360, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001475 DAOSPL010000000
VERSION     DAOSPL010001475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100776_length_1333_cov_2.430360"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="PLQ54_20745"
     CDS             complement(<1..1118)
                     /locus_tag="PLQ54_20745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95748.1"
                     /translation="MMTGHADRPRCAGLCGWLTLVALMLLPTAATAQNLVTLADFEDG
                     VGPWKVEADRIDAIGTEPAGLARVAPAEKTHALGGHSAGAFTFATASRAFARIALPID
                     GAALAAAGAHTLSFWLRPYGPAASVQMVLSGVEPSGIEHRYVATLKLSEVPWTQVMAP
                     LTKFTEDKQPMGAGLKWVSSFSFYKRGDWQGFTFLLDDLRMDCARPLAPSAVQPPSQP
                     LGPDTPPRPDVPPPLPTGPEVPSEAAPVTEGPLLPAPGAPYDVTVNVRFDKPLQIGWR
                     PAIGASVGLADASALQDPRVARRLQAAGLKLLRIRVPVAPGGMLSPQDLAALDAIVAT
                     RPKLGNPTLMVCLDDPYRSKGSADLVAVATTIARRFNG"
BASE COUNT          232 a          444 c          461 g          196 t
ORIGIN      
        1 tccccgttga agcgtctggc gatggtggtg gcaaccgcaa ccaggtcggc agaccctttg
       61 ctccggtatg gatcatcgag gcagaccatg agcgtggggt tccccagctt gggccgggtt
      121 gccacgatgg catccagcgc tgccagatcc tgcggagaca gcatgccacc cggggccact
      181 ggcacgcgga tccggagcag cttgagaccg gccgcctgca gacgccgcgc gacgcgtgga
      241 tcctgaagcg ccgaggcatc tgccaggccc acggaggcgc ctatggctgg cctccagccg
      301 atctggaggg gcttatcgaa cctcacgttc acggtaacgt catacggcgc cccgggtgcc
      361 ggcagaagtg ggccctcggt gaccggtgcg gcctcggaag gcacctcggg cccggtgggg
      421 agtggcgggg gaacgtcggg acgcgggggc gtatcgggcc ccaaaggctg ggacggcggt
      481 tggactgccg atggcgccag cggccgcgcg caatccatcc gcaggtcatc gaggaggaac
      541 gtgaaccctt gccagtcacc acgcttgtag aaggagaacg acgacaccca cttgagccca
      601 gcccccatgg gctgcttgtc ctcggtgaac ttggtcagcg gcgccatcac ttgcgtccag
      661 ggcacctcgg acagtttgag cgttgccaca taccggtgtt cgatcccact gggctcaaca
      721 cccgacaaca ccatttggac cgaagcggca gggccatagg ggcgcagcca gaacgagagc
      781 gtatgagctc cggccgccgc cagggcggcc ccatcgatgg gcagcgcgat gcgagcgaaa
      841 gcccgactgg ctgtagcgaa ggtgaatgct cccgcggagt gcccgccgag ggcgtgggtc
      901 ttctccgccg gggcgacccg ggccagtccc gcaggctccg tccctatggc atcgatgcgg
      961 tcggcctcca ccttccaggg gccaacccca tcctcgaagt ctgccagtgt caccagattc
     1021 tgcgcggtcg cggcggtggg gagtaacatg agcgccacca gcgtcagcca cccgcacagc
     1081 cccgcgcaac ggggtcgatc ggcgtgtccg gtcatcacgg cacagcggcc tcccactgga
     1141 gagagtcccg tggcacggaa gcctcgaggg tgcctcgagt gccgccggca ggggctcgaa
     1201 ccccacccaa catggttggt tcgggtggtc acgagagcct tcctctctgg tctcagaagg
     1261 tcacagaggg ccccgcggtt cgctgaccgg gtggcacaag ccgcccccac gccagatgga
     1321 acggggagga tgc
//
LOCUS       DAOSPL010001476         1330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_101083_length_1330_cov_3.287843, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001476 DAOSPL010000000
VERSION     DAOSPL010001476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101083_length_1330_cov_3.287843"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..>1330)
                     /locus_tag="PLQ54_20750"
     CDS             complement(96..>1330)
                     /locus_tag="PLQ54_20750"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOS95749.1"
                     /translation="RFRRLLMFSYAPRANGHVLNPPVALQCFDRYSWTRADWATEAGQ
                     LDYVKTAARLGCDSAWLDAAWFPGGFPNGVGNWSAKPVEFPNGLRPVGDACHANGLRF
                     VLWFEPERVAPGTQIATEHPEFVFGGRDGGLFRLDLPEAQQWLLELLSSRIREYGIDV
                     YRNDFNIDPLGYWRANDTPDREGMTEIRYVENHYAMWDELLRRHPGLMIDNCASGGRR
                     IDLETISRSVPLWRSDTSCSPGHADWDQAQACGISLYVPLHTACAWDSRAYTVRSSAT
                     MGMAFQPEYMAPTFDEALAQRTLAEVRRNAPFWYGDLYPLTAITNTPEHWMAWQMHRP
                     DLNAGIVLAFRRAECPYPIIALSLRALDPETRYTVASCGEDGQETTQTLTGAELSSDM
                     EIRLARGASLVMRYEPAE"
BASE COUNT          216 a          437 c          453 g          224 t
ORIGIN      
        1 acaccctggt ggtggacggc atccacggtt tcgtcgtcgt gaaccccgcc cgggcattcg
       61 cgatacgccg ccctgttgcc agtccgtagc ggccgctact ccgccggctc gtaacgcatg
      121 accaggcttg cgccgcgggc cagacggatc tccatgtccg acgagagctc ggccccggtc
      181 agcgtctgtg tggtctcctg cccatcctcg ccgcacgagg ccaccgtgta ccgtgtttcc
      241 ggatcgaggg cccgcaggct cagcgcgatg atggggtacg ggcattcggc ccggcggaaa
      301 gccagcacga tgcccgcgtt gaggtccggc cggtgcatct gccacgccat ccagtgctcg
      361 ggtgtgttcg tgatcgccgt caatgggtac aggtccccgt accagaaggg cgcgttccgg
      421 cgaacctcgg ccagggtccg ctgagccagg gcttcgtcga aggtcggcgc catgtactcg
      481 ggctggaagg ccatgcccat ggtggcagag ctccgcaccg tgtaggctcg cgaatcccac
      541 gcgcacgcag tgtgtagggg cacatagagg ctgataccgc aggcctgggc ctggtcccaa
      601 tcggcgtggc cgggcgagca gcttgtgtcg ctgcgccaca ggggcaccga gcgagagatg
      661 gtctcgaggt cgatgcgtcg tccgccgctc gcgcagttgt cgatcatcag gccaggatgc
      721 cggcgcagaa gctcatccca catggcgtag tgattctcga cgtaacggat ctccgtcatg
      781 ccctcccggt ctggcgtgtc gtttgcacgc caatagccca gcgggtcaat gttgaagtcg
      841 ttgcggtaga cgtcgatgcc gtactcgcgg atccgactgg agagcagttc gaggagccac
      901 tgctgggcct cgggcaggtc cagccggaac agaccgccgt cccggccgcc aaacacgaac
      961 tcggggtgct cggtggcgat ctgggttccc ggagccaccc gctcgggctc gaaccacaga
     1021 acgaagcgca ggccattggc gtggcatgca tcgccgacag gcctcagacc gttcggaaac
     1081 tcgaccggct tggccgacca gttcccgaca ccgttgggga agccgccggg gaaccaggcc
     1141 gcgtcgagcc aggcgctgtc gcagcccagc ctcgcggcgg tcttgacgta gtcgagctgg
     1201 ccggcttccg tggcccaatc ggcccgggtc cagctgtagc ggtcgaagca ctgaagtgcc
     1261 accggcgggt tgagcacgtg accgtttgct cggggggcgt agctgaacat cagcaagcgc
     1321 cggaaccgta
//
LOCUS       DAOSPL010001477         1330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_101144_length_1330_cov_1.914510, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001477 DAOSPL010000000
VERSION     DAOSPL010001477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101144_length_1330_cov_1.914510"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="PLQ54_20755"
     CDS             complement(<1..1089)
                     /locus_tag="PLQ54_20755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOS95750.1"
                     /translation="MSRPIVIGLAGNPNAGKTTVFNALTGAHQHVGNYPGVTVEKKEG
                     IATHAGRELRIIDLPGTYSLTAYSMEEIVARDFVVHEKPDVIIDVVDASNLERNLYLT
                     TQFIELQQPTVVALNMIDVAEMSGKRIDHELLGELLGVPFVPCVAARGQGMRELLDAA
                     IRVADENRKPALHMRFGSELDCHVDQIEAMLVASELQSDAAPRWLAIKLLENDARVRE
                     MVEKQAAGADEILAAVDSARSHIERIVGDDAEVALADQRYGLASGAVRQAMQHDQTRR
                     IDWSDAVDRVLVDRVLGLPIFVLFMWLMFEMVFRLGGPPMEWVEAFFGALAGYASSVL
                     PDGDLKSLIVDGLIGGVGGVLVFVPNIAL"
     gene            complement(1151..>1330)
                     /locus_tag="PLQ54_20760"
     CDS             complement(1151..>1330)
                     /locus_tag="PLQ54_20760"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOS95751.1"
                     /translation="SVEGSVGSRRHLSDLGILPGTRMQVVSGGGQPGPVIINVRGAKL
                     MLGRGMAHRIMVRPD"
BASE COUNT          242 a          415 c          453 g          220 t
ORIGIN      
        1 gagcgcgatg ttgggcacga agacgagcac cccgccgacg ccgccaatga gcccgtcaac
       61 gatgagggac ttgaggtcgc catccggcag gacagaggac gcgtagccgg cgagggcgcc
      121 gaagaaggcc tccacccatt ccatcggcgg cccgcccagg cggaagacca tctcgaacat
      181 gagccacatg aacaggacga agatgggcag acccaggacg cgatccacga gcacgcggtc
      241 cactgcatcg gaccagtcaa tgcggcgggt ctggtcgtgc tgcatggcct gccgcacggc
      301 gcccgatgcc agaccgtagc gctggtcggc gagggcgacc tccgcatcgt cccccacgat
      361 gcgctcgatg tgagagcggg ccgagtcaac ggccgcgagt atctcgtcag cacccgcggc
      421 ttgcttctcc accatctcgc ggacccgcgc gtcgttctcg agcagcttga tggcgagcca
      481 gcgcggggca gcatcgctct gcagttcgct ggcgaccagc atggcctcga tctggtccac
      541 gtgacagtcc agttcggagc cgaatcgcat atgcagcgcg ggtttgcggt tctcgtcggc
      601 aacgcggatg gcggcgtcta gcaactcccg catgccctgc ccacgcgccg cgacacacgg
      661 gacgaagggc actccgagca gttcacccag gagttcgtga tcgattcgct tgccggacat
      721 ctcggcgacg tcgatcatat tgagggctac gacggtaggc tgctggagct cgatgaactg
      781 ggtggtgaga tacaggtttc gttcgagatt ggaggcgtca accacgtcga tgatgacgtc
      841 aggcttctcg tgcaccacga agtcccgggc gacgatctcc tccatggaat aggcggtgag
      901 gctgtaggtg ccggggagat cgatgatccg cagctcgcgg cccgcgtggg tggcaatgcc
      961 ctctttcttc tcgacggtca cgccggggta gttgcccaca tgctggtggg cgccggtgag
     1021 ggcgttgaag actgtggtct tgccggcatt ggggttgccc gcgagaccga tgacaatggg
     1081 gcgggacatg gggcacacca ctctcgatgg gaaacgcggc gccgcgggga gcgcctcagc
     1141 agaaaaaggc tcagtcaggt ctgaccatga tccggtgggc cattccgcgg ccgagcatga
     1201 gcttggcgcc gcgtacattg atgatgaccg ggccgggctg gccgccgccg ctgaccacct
     1261 gcatgcgggt tcctggaagg attcccaggt cactaagatg cctgcgggag ccgacgctgc
     1321 cctccacgga
//
LOCUS       DAOSPL010001478         1329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_101214_length_1329_cov_2.551020, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001478 DAOSPL010000000
VERSION     DAOSPL010001478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101214_length_1329_cov_2.551020"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..984)
                     /locus_tag="PLQ54_20765"
     CDS             complement(148..984)
                     /locus_tag="PLQ54_20765"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOS95752.1"
                     /translation="MKYGVNFLLWSASNKDDARWVRKAAAMGFDMVEIAVFDPAGVDV
                     PAVRAALKDTGVALSLCSIMTPERDLTSEDPALRETAKEYIRGTVLLGEQLGAKLFCG
                     PLYAPVGKLVGRGRTPEEWDRVVAGLREVAGFAHDHGMCLCIEPLNRFETYFINIAAD
                     ARRMVDEVASPGLAMHLDTFHMAIEEKSSADAIRLAGKRLRHVHFCENDRGAPGTGQV
                     RWSEIFSALRQIGYDGGAVIESFVPGIPEIAAAAAIWRDIEPSADDTATKGLEFMRAH
                     GW"
BASE COUNT          205 a          455 c          459 g          210 t
ORIGIN      
        1 agcgctggcg atgcgtgctc aacggcggag ggggccccga gtacgactcg atcgtcggcg
       61 acagacccgt gtgactggcc gacggatcag ccaccgatct cgccgtacgt gaccgcgtcg
      121 ccttccgcgt gacccacacg ccgcgcacta ccacccatgt gcgcgcatga actcgaggcc
      181 tttggtcgcg gtgtcgtcgg cgctgggctc gatgtcgcgc cagatggcgg ccgcggccgc
      241 tatctcgggg atgccgggca cgaaggactc gatgaccgcc ccaccgtcgt agccgatctg
      301 acgcagcgcc gagaagatct cgctccaccg cacttggccc gtgcccggcg cgccgcgatc
      361 gttctcgcag aagtgcacgt gcctgagccg cttgccagcc aggcggatgg cgtcggccga
      421 gctcttctcc tcgatggcca tgtggaaggt gtccaggtgc atggccaggc cggggctggc
      481 gacctcgtcc accatgcggc gggcgtcggc ggcgatgttg atgaagtagg tctcgaagcg
      541 gttcagcggc tcaatacaca ggcacatgcc gtgatcatgg gcgaagcctg ccacctcacg
      601 cagaccggca acgacgcgat cccactcctc gggcgtgcgt cctcggccca cgagcttccc
      661 cacgggagcg tacagggggc cgcagaagag ctttgcgccg agctgctcgc cgagaagcac
      721 cgtgccgcgg atgtactcct tggccgtctc gcgcaatgcc ggatcctcgg aggtgaggtc
      781 gcgctccggc gtcatgatgc tgcacaggga gagcgcaact ccggtgtcct tcagcgcggc
      841 acggacggcg ggcacgtcga cgcccgcagg gtcgaacacg gcgatctcga ccatgtcgaa
      901 gcccatggca gcggccttgc ggacccaccg cgcgtcatcc ttgttcgagg cggaccagag
      961 caggaagttg acgccgtact tcatcggtgc acctccgggg cgagtgcgag ggtattcccg
     1021 gcggcggccg gaaggccctg cggcgcccgg cggtctctcg cgtaggtcga gcgtcctcgc
     1081 tcgacacctc agggtctcga cagcggagga tggagcgacc cccgccgcta tgcccaggat
     1141 gaccccagag gcgggccgga tggcgccctt cccgcttcgc gggccccttc cattcggccc
     1201 ctcctcgcca tcacggaggg tgttcggagg ggccgcatag gcaggatcag ccttaggctg
     1261 atgggaccct atccggccat tggcggtaac atagggccct gggccgggcg gctgggtctc
     1321 gcgaaggtc
//
LOCUS       DAOSPL010001479         1327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_101470_length_1327_cov_2.264151, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001479 DAOSPL010000000
VERSION     DAOSPL010001479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101470_length_1327_cov_2.264151"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1327)
                     /locus_tag="PLQ54_20770"
     CDS             complement(<1..>1327)
                     /locus_tag="PLQ54_20770"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOS95753.1"
                     /translation="RRILEMCVEAGGTITGEHGVGSEKTAALRLVFGPTELELMRRIR
                     RVFDPEARSNPGKVFDEPPMPRPKPPTRGLAEFVDQLRDIVGAEAVSGECDIRAAHAM
                     DGLVPSFVVWPSTPEQVAAVLGACWAEGAAVGVAGGGTRRSCWAPLSAYDVCLSTGRL
                     DGVIEHRSANLSLSVGAGMTLAAAQDHVGAACQRLPLAASYPDFVSVGGLLARRASGP
                     EELRFGPVQHRVLGLRVALGDGSLARLGGRCMKNVAGYDVAKAILGSCGTLGVICEAT
                     LALQPAPEAARWVQAEYASMGGAFQSARALARDAVELWALEVAYPRPAAYALVCGTEE
                     DATRRARRCVELMEGSGAQSASILSQREAARLWPELRDFPGPPGRPVLPPSHAAGLEV
                     RVPPAACPDLAARLPEVEARHRLEAGLLAGAGTGLVRVRGGGRRLAAKSQ"
BASE COUNT          208 a          519 c          435 g          165 t
ORIGIN      
        1 ctgcgacttt gctgccaacc gcctgccgcc tccgcggacg cgcaccagcc cggttcccgc
       61 cccagccagg agcccggctt cgagtcggtg gcgggcctcg acctccggca agcgcgcggc
      121 caggtcgggg cacgcggccg gcgggactcg cacctccagc ccagccgcat gggacggcgg
      181 caagaccggc cgcccggggg ggcccggaaa gtcgcgaagt tcgggccaca accgtgccgc
      241 ctcgcgttgc gacaggatcg acgccgactg cgccccggag ccttccatca gctcgacgca
      301 ccggcgcgcg cgccgcgtag cgtcctcctc cgtgccacac acaagcgcgt aggccgccgg
      361 gcgagggtag gccacctcaa gtgcccacag ctcgaccgca tcccgcgcca gtgcgcgggc
      421 agactggaat gccccgccca tgctcgcgta ctcggcctga acccatcgtg cggcttcggg
      481 ggctggttgc agcgccagcg tcgcctcgca gatcacgccc agggttccac atgagcccag
      541 gatggccttg gccacatcgt accccgccac attcttcatg cagcgtccgc ccagccgcgc
      601 gagggagcca tcgcccaacg cgaccctgag gcccaagacc cggtgctgaa ccggtccgaa
      661 gcgcagctcc tccggccccg aggcccggcg ggcgagcaac ccgccgaccg aaacgaagtc
      721 gggatagctc gcggccagcg ggagccgctg acaggccgcg ccgacgtgat cctgtgcggc
      781 tgcgagcgtc atgccggcac ccacggatag gctcaggttc gccgaccggt gctcgatcac
      841 gccgtccagc ctgcccgtgg agaggcacac gtcgtatgcc gacagcgggg cccaacagct
      901 ccggcgcgtc ccgccaccag ccacgcccac cgcagcgccc tccgcccagc acgcacccag
      961 gacggcggcc acctgttcgg gtgtggacgg ccagaccacg aaactgggca cgagcccatc
     1021 catggcatgg gccgcccgga tgtcgcattc acccgagacg gcctccgcgc ccacgatgtc
     1081 gcggagctgg tccacgaact cggcgaggcc gcgcgtcggt ggcttgggcc gaggcatggg
     1141 cggctcatcg aacacctttc cggggttcga tcgcgcctcg gggtcaaaca cgcgccggat
     1201 ccggcgcatg agctcgagct ccgtcgggcc gaacaccagc cggagtgctg cagtcttctc
     1261 gctgcccaca ccgtgttcgc ccgtaatggt gccgccggcc tcgacacaca tctcgaggat
     1321 acggcgc
//
LOCUS       DAOSPL010001480         1326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_101567_length_1326_cov_2.422502, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001480 DAOSPL010000000
VERSION     DAOSPL010001480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101567_length_1326_cov_2.422502"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PLQ54_20775"
     CDS             complement(<1..428)
                     /locus_tag="PLQ54_20775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041932144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOS95754.1"
                     /translation="MGDVACDWLERLIWTKARAASLWPMTFGLACCAIEMMCTFASRF
                     DLDRFGVFPRASPRQSDVIIVSGTLTHKMASRLRRLYDQMPEPKYVISMGSCANCGGP
                     FRAGYHVVRGVDLVVPVDVYVPGCPPRPEALLQGFEELRK"
     gene            complement(533..922)
                     /locus_tag="PLQ54_20780"
     CDS             complement(533..922)
                     /locus_tag="PLQ54_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012059188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOS95755.1"
                     /translation="MGPGIDSRDYVQAFIFLIGALVFLLVPLGLALLFRPNQPGGLKH
                     TTYECGEEPIGSAWFRHPIQYYLIALVFIVFDVEAIFILPWAVQARALIREGLGLFAF
                     TEMTIFAGILLLGWYYALRKGALEWLK"
BASE COUNT          248 a          457 c          436 g          185 t
ORIGIN      
        1 ttcttgcgga gttcctcgaa gccctgaagc agcgcctcgg gccgcggcgg gcagcccggc
       61 acatacacat ccacgggcac cacgaggtcc acaccccgga ccacgtggta ccccgcgcga
      121 aacgggcccc cgcagttagc acagcttccc atcgagatca cgtacttggg ctcgggcatc
      181 tggtcataca agcgcctcaa ccgcgaagcc atcttgtgcg tcagcgtgcc cgagacaatg
      241 atcacatccg attggcgggg agaggcgcgg gggaagacac cgaagcggtc caggtcgaaa
      301 cgcgacgcga aagtgcacat catctcgatg gcgcagcagg ccaggccgaa ggtcatgggc
      361 cacagcgatg cagcgcgcgc tttggtccat atcagccgct ccagccagtc gcaggcgaca
      421 tctcccatgg ggagcttcgc cagcacgcta ctgcggaggg ggttcaccca ctcgggggca
      481 tcgtcgggca gcgcgtcggt gcggatgagc ccgccggcgg ccggtccgtg ctctacttca
      541 gccactcgag agctcccttc cgcaaagcgt agtaccaccc caagaggagg atgcctgcga
      601 agatggtcat ctcggtgaac gcgaacagac ccaatccctc gcgaatcaag gcccgagcct
      661 gaaccgccca gggcaagatg aagatggctt cgacgtcgaa cacgatgaac accagagcga
      721 tcaggtaata ctggatcggg tggcggaacc acgcgctgcc gatgggctcc tcgccgcact
      781 cataggtcgt gtgcttgagc ccgcccggct ggttcgggcg gaacagcagg gccagcccca
      841 gcgggacgag caggaatacc agcgcgccaa tcaggaagat gaacgcctgg acgtagtcgc
      901 ggctgtcaat gcccggaccc atggccaccc tcctcggctg ccatcttccg tgggaggccg
      961 cgcgggtcct ctcgccgcag ctccccaccg acacagacct cgggactctg ccccattggc
     1021 gctgacctca ggctcggatg acgcctcagg agcgccgaga cggcccaccg ggggcttgcc
     1081 gagcggcgcc acggggggag aacacgcgag cttcagcgtg gtgggccccc aacgcgaccg
     1141 atctggcggc cgggatgcgg cgcgcagccg cgttcgggcg cgcgccgtcc atgtggccga
     1201 cggggccttc gacaggctca ggccaggggc ctggcgtcct ccagcacacc gcgtggtgcc
     1261 gtgtcggagc ctgccgaatc tgctcctaag cccaggcagc cgccgcggcc agtccggtgt
     1321 gacgca
//
LOCUS       DAOSPL010001481         1324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_101747_length_1324_cov_3.162333, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001481 DAOSPL010000000
VERSION     DAOSPL010001481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101747_length_1324_cov_3.162333"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PLQ54_20785"
     CDS             complement(<1..753)
                     /locus_tag="PLQ54_20785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOS95756.1"
                     /translation="MTMDSLLQQLQTYVIPTPGRAPIAFVRGEGMKLWDVEGRCYTDF
                     LAGIAVCGLGHCHPKVVEAIQKQAATLMHTSNLYIIPPQMELAERLAGTCDLKRSFFA
                     NSGAEANEAAIKIARKYGRTHGGESKTRIIAAHNSFHGRTTGALAATGQPKYQSAFEP
                     LIPGFTFVDYADAAAVARAMDDTVCAVLVEPIQGESGVRVPPDGYLTELRKLCDRYDA
                     LLMLDEVQTGLGRTGYWYGYMHDGAIPDVMTLA"
     gene            complement(874..>1324)
                     /locus_tag="PLQ54_20790"
     CDS             complement(874..>1324)
                     /locus_tag="PLQ54_20790"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="catalyzes the phosphorylation of
                     N-acetyl-L-glutamate to form N-acetyl-L-glutamate
                     5-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOS95757.1"
                     /translation="DLGFVGQIESINAGLLSALTEDGFIPVVCSIAGGADGECYNINA
                     DHAAGRIAAALHAEKLLSLTDVRGLLRDRSDPASLIARLTVSEAEALVREGQVEGGMV
                     PKLESCVDAVKAGTAKAHIVDGRIEHALLMEVFTDAGIGTMVVPDGP"
BASE COUNT          214 a          421 c          466 g          223 t
ORIGIN      
        1 ggccagggtc atcacgtcgg ggatggcgcc atcgtgcatg tacccgtacc agtagccggt
       61 gcggcccaag cccgtctgaa cctcgtccag catgagcagc gcgtcgtacc ggtcgcagag
      121 cttgcgaagc tcggtgaggt agccgtcggg cggcacgcgc acgccgctct ccccctggat
      181 gggctcgacg agcacggcgc agacggtgtc gtccatcgcg cgcgcaacgg cggcggcatc
      241 ggcgtagtcg acgaacgtga agccggggat caggggctcg aaggctgact ggtatttggg
      301 ctggcccgtg gccgccaggg caccggtggt acggccatgg aaggagttgt gggccgcgat
      361 gatgcgggtc ttgctctcgc cgccgtgcgt ccggccgtac ttgcgggcga tcttgatggc
      421 cgcttcgttg gcctcggcgc cgctgttcgc gaagaacgat cgcttgaggt cacacgtgcc
      481 ggccagccgc tcggcgagct ccatctgagg cgggatgatg tacaggttcg acgtgtgcat
      541 gagcgtcgcg gcctgcttct ggatggcctc gacgaccttg ggatggcagt ggccaaggcc
      601 acacaccgca atgccggcca ggaagtcggt gtagcagcgg ccctccacat cccacagctt
      661 catgccctcg cctctcacaa acgcgatggg cgcgcggccg ggagtgggga tcacataggt
      721 ctggagctgc tggagcagag agtccatcgt catggtgtgt cgcccttcat gtgcgtgtcg
      781 gcgtgcgtgg aatacctgcg ggtgccgggc agcggtcagg gccacctcgc ccggtcgcgg
      841 cgtgagggtg ctgccatcca ggcggcgcgg aggctacggg ccgtcgggga cgaccatggt
      901 accgataccg gcatcggtaa acacttccat cagcagagcg tgctcgattc tcccatccac
      961 aatatgggct ttggcggtcc cggctttcac cgcgtcgaca caagactcga gcttcggcac
     1021 catcccaccc tcgacctggc cctcgcgcac gagcgcctcg gcctcggata cggtgagccg
     1081 ggcgatgagc gaggcagggt cgctgcgatc gcgcagaagc ccgcggacat cggtgagcga
     1141 gagcaacttc tcagcgtgaa gggcggccgc gatccggccg gcggcatggt cggcgttgat
     1201 gttatagcac tcgccgtccg cgccgccggc aatggagcag accacgggga tgaagccatc
     1261 ctccgttagc gcgctcagca gaccggcgtt gatggactcg atctggccca cgaagcccag
     1321 atcg
//
LOCUS       DAOSPL010001482         1323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_101855_length_1323_cov_3.641167, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001482 DAOSPL010000000
VERSION     DAOSPL010001482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101855_length_1323_cov_3.641167"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..270)
                     /locus_tag="PLQ54_20795"
     CDS             complement(109..270)
                     /locus_tag="PLQ54_20795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95758.1"
                     /translation="MSRSTRREFIRNTAALGAVGVAAAKAASAQTRRKRWSPNDRIHV
                     GLIGCGGMG"
     gene            complement(465..>1323)
                     /locus_tag="PLQ54_20800"
     CDS             complement(465..>1323)
                     /locus_tag="PLQ54_20800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95759.1"
                     /translation="ETFQVAELASEGDPAFGVVKTLTVEYQVGERSQTASATDPEFIT
                     FELPSDAPYPVRLRRTPTGGLWAEASQPGLYTATLRSGKRITFRGAASRYTDILGPWD
                     LGFPPNWGAPEKVKLESLASWSDHPDEGVRYFSGTATYTNTFRLSRGTLGRGRVAYLD
                     LGKVEVMARVRLNGKDLGILWRAPYRANISNAAKVGDNQLEIEVTNLWPNRMIGDEQL
                     PEDSDRHGNGTLKAWPEWLAGDGPSPTGRFTFTSWRLWAKDSPLQPSGLLGPVRVISV
                     GTTTGESGK"
BASE COUNT          209 a          472 c          425 g          217 t
ORIGIN      
        1 gcaggttgtt gggtgctccg cgaccacggc ctcgcgcctc tcgggccaaa catcgcaggc
       61 accggtaacg accacatcag gatgcgaagc gcatgcgccc aagttggctc agcccatgcc
      121 gccgcaaccg atcaggccca cgtggatgcg atcgttcggg ctccagcgct tgcgccgtgt
      181 ctgcgcgctg gctgcctttg ccgcagccac gcccacggcc cccagcgcgg ccgtgttccg
      241 aatgaactcg cggcgggtgg atcgactcat ctccgacacc tccggtgtgt gcgtcaacgt
      301 agcgggcagg tggcacagat cgggccggat ggcgccctta ccgctccacg cgcccctctc
      361 gtccggcccc tcctctggag ccacatgcgg tgttcggagg ggccggatag ggcggatcag
      421 ccgaaggctg atgggacccg atccggccca atgccccggg acccctactt gcccgattcc
      481 cctgtcgtcg tccccaccga gatgacgcgg accgggccga gcaagcccga cggctgcagc
      541 ggggaatcct tggcccacag gcgccacgag gtgaaggtga accggccggt ggggctaggg
      601 ccgtcgcccg ccagccattc gggccaggcc ttcagcgtgc cgttcccatg gcggtcgctg
      661 tcctcgggga gctgctcgtc gccgatcatc cggttgggcc acagattggt gacttcgatc
      721 tcgagctggt tgtcgcccac cttggccgcg ttgctgatgt tggcccgata cggcgctctc
      781 caaaggatgc ccaggtcctt cccattgagc cggacccgcg ccatcacctc gaccttgccg
      841 aggtcgagat aggccacgcg tccacggccc aaggtgcccc gcgacagccg gaacgtgttc
      901 gtgtacgtcg ccgtacccga gaagtagcgc acaccctcgt ccgggtggtc gctccacgag
      961 gcgagcgact cgagcttcac cttctcgggc gcgccccagt tcggcgggaa gccaagatcc
     1021 cacggcccca ggatgtcggt gtagcgcgac gcggcacccc ggaacgtgat ccgcttgccc
     1081 gaacgcagtg tggccgtata gagccccggt tgcgatgcct cagcccatag cccacccgtc
     1141 ggcgtgcggc gaagtcgaac cggatacggg gcatccgatg gcagctcgaa ggtgatgaac
     1201 tcgggatcgg tggccgaagc cgtctgcgaa cgctcgccaa cctgatactc gacggtgagg
     1261 gtcttcacca ccccgaatgc cgggtcgccc tctgacgcca gttccgccac ctggaacgtc
     1321 tca
//
LOCUS       DAOSPL010001483         1322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_101975_length_1322_cov_3.600631, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001483 DAOSPL010000000
VERSION     DAOSPL010001483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101975_length_1322_cov_3.600631"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            350..>1322
                     /locus_tag="PLQ54_20805"
     CDS             350..>1322
                     /locus_tag="PLQ54_20805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOS95760.1"
                     /translation="MQASVVVLTGVLTAAASFAQVGPTEKMEITVYNQNFGLVKDVRS
                     IDLPEGRSVIQFMDVAAQIDPTSVHFKSLTDPTGVEILEQNYQFDLVDRAKLMQKYLG
                     KEIRITRYDQDGQPVETQEGVLLGAEGGRPGVVRIGDEIVLDPQGTVMLPKLPEGLII
                     RPTLVWDLAAAKAGKHRVELSYMTGGLTWAADYVAVVNQDDTKADLNGWVTLTNTSGA
                     RYPDATLKLIAGDVRRVEEPQVRRRVRATAAPAPAGGQPGFEEKAFFEYHMYTLQRPA
                     TIAENETKQVTLLGAPNVAINKVYVFDPATAPVVYSPTGDNNKRKVQV"
BASE COUNT          254 a          426 c          429 g          213 t
ORIGIN      
        1 agtgccacat ggaccgctcc gcgggctagc ggcagctaca tcgtggcatg ccaagtggcc
       61 gacggacagg gaccaagccg cctatactac cgaacggtga ttgtgcagta gtcgatcagc
      121 agtggctctc caggagctcg gctaccgagc cctctcgttg gcttcatatc tgctcgtgga
      181 acctcaggta ccctggggcc ggtcccacca gaggggcatc aacgggcagg cggcgaacca
      241 ccaccctcga gtctcgcgtc gggcgggcct cgtcagccct ccgcctgctg tgatcgtccc
      301 tgttcgctgg aagctgtgca tctggaacca atgggagtga ggcggcatca tgcaagcgtc
      361 agtagtggtg ctcaccggag tgctcaccgc agcagcaagc ttcgcccagg tcggcccaac
      421 ggagaagatg gagatcactg tctacaacca gaacttcggt ctggtgaagg atgtccggtc
      481 catcgacctc cctgagggcc gctcggtcat ccagttcatg gacgtggcgg cccagatcga
      541 cccgacgtcg gtgcacttca agtcgctgac tgatccgacg ggtgtggaga tcctcgagca
      601 gaactatcag ttcgacctcg tcgaccgcgc caagctgatg cagaagtacc tgggcaagga
      661 gatccgtatc acgcggtatg accaggacgg tcagcccgtg gagacgcagg aaggcgttct
      721 gctgggtgcc gagggcggcc ggccgggcgt ggtccgcatc ggcgacgaaa tcgtgctcga
      781 cccgcagggc acggtcatgc tgccgaagtt gcccgaggga ctgatcatcc ggccaactct
      841 tgtctgggat ctggcggccg ccaaggccgg caagcatcgg gtcgagctgt cctacatgac
      901 cggaggcctg acctgggcgg ccgactacgt cgccgtggtg aaccaggatg acacgaaggc
      961 cgatctcaat ggctgggtca cgctgaccaa caccagcggc gcccgctacc ccgacgccac
     1021 gctgaagctc attgcggggg acgtgcggag ggtcgaggag ccccaggtcc gccggcgcgt
     1081 acgcgccacc gcggcccccg cgccggcggg cggccagccg ggcttcgagg agaaggcgtt
     1141 cttcgagtac cacatgtaca cgctgcagcg ccccgccacc atcgccgaga acgagacgaa
     1201 gcaggtcacc cttctcggag cgcctaacgt ggccatcaat aaggtctacg tcttcgaccc
     1261 ggcgacagcg ccggttgtct actcgcccac tggcgacaac aacaaacgca aggtgcaggt
     1321 ga
//
LOCUS       DAOSPL010001484         1322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_102029_length_1322_cov_2.330702, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001484 DAOSPL010000000
VERSION     DAOSPL010001484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102029_length_1322_cov_2.330702"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PLQ54_20810"
     CDS             <1..826
                     /locus_tag="PLQ54_20810"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOS95761.1"
                     /translation="DRGVSAVGALRRAQRAVQLVAALGVAGGALALVAWCPRPLVLAS
                     YATRLEGRSASQIHNAALAARSLDGQVIGPGDAFSFGATVGPWTADMGFDKAPVSYEG
                     ELVLAYGGGVCQTSTTFYNAALAGGLEILERHPHQWPPKYVAPGRDAAVAYGEVDLRV
                     RNPYDFPITIRAATDRQSVHVSLLADRRPPFRVRVVQSIRGVDAPSVVIHYDARYARG
                     VRRTDVAGRPGIQVTTSREFIEGDRVVRRELVSHDRYEPVNTVMGIGTGPTGIPVR"
     gene            871..>1322
                     /locus_tag="PLQ54_20815"
     CDS             871..>1322
                     /locus_tag="PLQ54_20815"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS95762.1"
                     /translation="MAVRLGFIGVGGIASRHLQAAKARDDVQIIGHADVDPARARAAA
                     ESYGGTAYATASELYEEAQPDAVVICTPPYAHGDIETEACERGIHFFVEKPVAVNLDT
                     AAAVLSAVQASGVFTQVGYMYRLSPPLQRVRELPAGQELAYPLERGREA"
BASE COUNT          176 a          460 c          473 g          213 t
ORIGIN      
        1 agatcgggga gtgagcgccg tgggggcttt gcggagggcc cagcgggccg tgcagctggt
       61 cgcggccctg ggggtagccg gcggggcgct ggctctcgtc gcttggtgcc cgcgccccct
      121 cgtgctggcc agctatgcca cccgcctcga gggccggtcg gcgtcccaga tccacaacgc
      181 ggctctggcc gctcgatcgc tcgatggcca ggtcatcggg ccgggcgatg cgttcagttt
      241 cggtgccact gtgggtccct ggacggccga catgggtttc gacaaggccc ccgtcagcta
      301 cgagggcgag ctcgtgctcg cctatggggg cggcgtgtgt cagacgtcga cgaccttcta
      361 caacgccgcg ctcgccggcg ggctcgagat cctggaacgc cacccgcacc agtggccgcc
      421 gaagtacgtg gcccctggcc gcgatgcggc cgtggcctac ggcgaggtcg acttgcgtgt
      481 tcggaacccc tatgacttcc cgattaccat ccgcgcggcc acggacaggc agtcggtcca
      541 cgtctcgctg cttgccgatc gccggccgcc gttccgcgtc cgggtcgtcc agtccattcg
      601 cggtgtggac gccccatcgg tggtcatcca ttacgatgcg cggtatgctc ggggcgttcg
      661 ccgcaccgac gtggccgggc gcccgggtat ccaggtcacc acgtcgcgtg agttcatcga
      721 aggcgaccgg gtggtccggc gggaactggt atcgcacgac cgctacgagc cggtgaacac
      781 ggtgatgggc atcggcaccg gccccacagg catcccggtc cgataggccg gccggtcgcc
      841 gggcggcgcc aggtcgctgg agggtcccca atggctgttc ggctcgggtt catcggcgtg
      901 ggcggtatcg cgtcacgcca cctgcaggcc gccaaggcgc gggacgatgt gcagatcatc
      961 ggccatgccg atgtggatcc cgcccgcgct cgcgcggcgg ccgagagcta cggcggcacg
     1021 gcctacgcta ccgccagcga gctctacgag gaagcccagc ccgatgccgt ggtcatctgc
     1081 acgccgccct atgcgcatgg agacatcgag accgaagcct gtgagcgcgg catccacttc
     1141 ttcgtcgaga agccggtggc ggtgaacctc gacaccgccg cggccgtgct gtcggcggtt
     1201 caggcctccg gtgtgttcac ccaggtgggc tacatgtacc gcctctcgcc cccgctccag
     1261 cgggtacgcg agctcccggc cggccaggag ctcgcgtacc cgctggagcg ggggcgagag
     1321 gc
//
LOCUS       DAOSPL010001485         1319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_102319_length_1319_cov_2.512658, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001485 DAOSPL010000000
VERSION     DAOSPL010001485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102319_length_1319_cov_2.512658"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLQ54_20820"
     CDS             complement(<1..302)
                     /locus_tag="PLQ54_20820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95763.1"
                     /translation="MRPTPMAQRGLPIPALVACVAVALAFSPWAVLSGDDRAAGPATD
                     PRHGRAVELALRLLPAIYPNEMRELTIRSVQPGSWERDRMVFAHWLGDDGHEHLAGA"
     gene            complement(352..>1319)
                     /locus_tag="PLQ54_20825"
     CDS             complement(352..>1319)
                     /locus_tag="PLQ54_20825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95764.1"
                     /translation="DPPQAQVLVQYDLSSAAASCGIKVYPPCLGPAPVVFPSEGNLGS
                     DRGHHIQSVRLQVCPTHMGPYAFALCAGETTASGQEHRGRAAKPALPRGAGMAIWPPA
                     YGGAGDDGPGSPYNDDLSGAVAEFQQQLGIPQGLGGLHWAPASGAGPDQVDAVFARMS
                     EGPAAVVYAAAHATSHLLDFEDGRVLDLEYVLSDCTIPDSQASHAISELQGNDLRECL
                     LVMFQGCHTATGHILSDAVVGKGAQAFVGFEGEPLLEYHDEFAHGFWPMASQPGKDLA
                     EALDLAVDYLLVVKGASGGFETMWCTDASLELFPPRFSGPCVRAR"
BASE COUNT          236 a          440 c          435 g          208 t
ORIGIN      
        1 gccccggcca agtgctcgtg cccgtcgtca ccaagccagt gggcgaacac cattcggtcg
       61 cgttcccagc tgcccggctg cacactccgg atagtcagct cccgcatctc gttcgggtag
      121 atggccggga gcagacgcag cgccaactcc acggctcgac cgtgccgcgg atcggttgcc
      181 ggacctgccg cacggtcgtc acccgagagc acagcccacg gtgagaatgc caaggccact
      241 gccacacaag cgacgagagc gggtatcggc aggcctctct gcgccatcgg cgtgggtctc
      301 acgctggatg gcctcccttc gatcgagcgt agacgacccg gccttgggcc actatcgtgc
      361 acggacgcat ggaccgctga agcgaggggg gaacagctcc aggctcgcat cagtgcacca
      421 catggtctcg aagccacccg acgcaccctt cacgacgagg aggtagtcca cggccagatc
      481 gagagcctcg gcgaggtcct ttcccggctg actagccata ggccagaagc cgtgcgcgaa
      541 ttcgtcgtgg tactcgagca agggctcgcc ctcgaagccg acgaaggcct gagcgccctt
      601 gccgacaacg gcgtcagaca gaatgtgacc ggtcgcagta tggcagccct ggaacatcac
      661 cagtaggcac tcgcgcaggt cgttgccctg tagctcggag atagcgtgcg atgcctggct
      721 atcagggatc gtacagtcgc tgaggacgta ctccagatcc agcacacgac cgtcctcgaa
      781 atccagcagg tggctggttg cgtgggcggc cgcgtacaca acggctgctg ggccctcact
      841 catcctggcg aaaacggcgt cgacttggtc gggcccggca cctgatgccg gtgcccagtg
      901 gagtccacct aagccctggg ggattccgag ctgctgctgg aactccgcta ccgcgccact
      961 cagatcgtcg ttgtacgggc tcccgggccc atcgtcacct gcgccgccgt aggcgggcgg
     1021 ccagatcgcc atccctgctc cacgagggag cgctggcttg gcggcccggc cgcggtgctc
     1081 ctgcccggag gccgtcgtct cgcccgcgca gagcgcgaat gcatagggcc ccatgtgggt
     1141 gggacagact tggaggcgga ccgactggat gtggtgtccc cggtccgatc ccaggttgcc
     1201 ctcagacgga aagacaaccg gggcaggccc gagacacggc gggtagacct tgatgccgca
     1261 ggaggcggcg gcgctagaca ggtcatactg cactagcact tgtgcttgag gagggtcgg
//
LOCUS       DAOSPL010001486         1318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_102397_length_1318_cov_3.666667, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001486 DAOSPL010000000
VERSION     DAOSPL010001486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102397_length_1318_cov_3.666667"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /gene="tgt"
                     /locus_tag="PLQ54_20830"
     CDS             <1..978
                     /gene="tgt"
                     /locus_tag="PLQ54_20830"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:NF013835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOS95765.1"
                     /translation="PGSHVIRDCGGLQRFMGYPGLILTDSGGYQVFSLQPLVRVDDQG
                     ARFRSHIDGAVHLFTPESVVEIQEALGSDIMMPLDQPVPWGTSPADARAITDRSDAWA
                     ARARAHHLRDGGRRDQALFGIVQGGFTAEGRAASVRRIVDIGFDGYAIGGLSVGEEKS
                     VMFGVLAETAPLLPAHLPRYLMGVGTPADLIRCIALGIDMFDCVLPTRLARNCAAYTS
                     EGRKHLRNAHYTSADVPLDPRCDCPTCQRYPVAYLRHLCMAKEILASRLLTYHNIHYY
                     LRLMERARDAISQCTLPELVSEMEDLYSSPATGSDPVRMGTKRRSLDYH"
     gene            965..>1318
                     /locus_tag="PLQ54_20835"
     CDS             965..>1318
                     /locus_tag="PLQ54_20835"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOS95766.1"
                     /translation="MITIDQVRQDSGVRTLIEKADAVMDAIGYTEHGMRHALLVSDTA
                     QSVLRGLGHDPRRCELAAIAGLLHDVGNVVGRTGHEAAGAMLAGQMLREMGMPLDEVC
                     DIMTAIGSHEDPRGLV"
BASE COUNT          222 a          447 c          418 g          231 t
ORIGIN      
        1 ccgggctccc atgtcattcg cgactgcggt ggcctccagc gcttcatggg gtacccgggc
       61 ctgatcctca ccgactcagg cgggtatcag gtgttctccc tgcagccgct ggtgcgggtc
      121 gatgatcagg gggcccgttt ccgatcccat atcgacggcg cggtccatct gttcacaccc
      181 gagagcgtgg tcgagatcca ggaggcgctg ggttccgaca tcatgatgcc gctggaccag
      241 cctgtcccct ggggcacctc cccggcggat gcccgggcga tcaccgaccg ctccgatgcc
      301 tgggctgctc gggctcgcgc ccaccacctg cgggacggag gacgacgcga ccaggccctc
      361 ttcgggatcg tccagggcgg tttcacagcc gaggggcgcg ccgctagtgt acggcgaatc
      421 gtcgacatcg ggttcgacgg ttacgccatc ggtgggctgt ccgtcggcga ggagaagtcg
      481 gtcatgttcg gcgtgctggc cgagacggcg ccactactgc cagcccatct ccctcggtac
      541 ctcatgggcg tcggaacacc ggccgatctc atacggtgta ttgcgctggg tatcgatatg
      601 ttcgactgcg tgcttccgac gcggcttgcg cggaactgcg ccgcctacac ttccgaaggc
      661 cgaaagcacc tgcgcaacgc acactacacg tcggccgacg ttcccctgga cccccgatgc
      721 gactgcccga catgccagcg gtatccggtg gcctacctgc gccatctatg catggccaag
      781 gagattctgg cctccagact gctgacctac cacaacatcc actactatct gcggctaatg
      841 gaacgcgccc gggatgccat ctctcagtgc acgctcccgg aactcgtctc ggagatggaa
      901 gacttgtact catcgcctgc gacgggatcc gacccggttc gcatgggcac aaaaaggaga
      961 tcacttgatt accattgacc aagtgcgcca ggactcgggc gtgcgcaccc tcatcgagaa
     1021 ggccgacgcc gtcatggacg ccatcggcta cacggagcac ggaatgcggc acgcgctgtt
     1081 ggtgtccgac accgcccagt cggtcctccg gggcttgggc cacgacccgc gccgatgcga
     1141 gctcgctgcc atcgccggac tgctgcacga cgtggggaat gtcgtcggcc gaaccgggca
     1201 cgaggccgcc ggagccatgc ttgccggaca gatgctgcgc gagatgggca tgcccctgga
     1261 cgaggtctgc gacatcatga ccgccatagg cagccacgag gaccctcgtg gcctggtg
//
LOCUS       DAOSPL010001487         1314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_102918_length_1314_cov_2.262907, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001487 DAOSPL010000000
VERSION     DAOSPL010001487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102918_length_1314_cov_2.262907"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1314
                     /locus_tag="PLQ54_20840"
     CDS             <1..>1314
                     /locus_tag="PLQ54_20840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95767.1"
                     /translation="PWRDAGRALAFFRPRYVGLMFKTFGNGPSVVTDVDSLSWTTKPV
                     DAAPISARVAVDSAQPGTPLDRNLFGHFTEHLGRCIYHDGIWAELVRNRKFVGDAGPD
                     GALASWRRFGSDDGVELARDNMTYYTGSQSQRITSRQAAGEHGIAQDGFRLRPVDYQC
                     RIVLRQTGDDAPVTVSLRRGDQVLAESRIDRPADKWRTYTSTLQPESPGPASLCITTE
                     AARGNLWIGAISLMPGDAVDGMRADLIEAIRALHPPIIRWPGGNFASGYHWRDGIGER
                     DLRPARFDRAWNVWDTNDFGTREFLRFCELVGCAPYICLNLGEGTPDEAVEWVQFCNG
                     AANTPGGRLRDDGGPREPYGVRYWGLGNELYGDWQLGHLTPTNYGRKAAEFARAVREV
                     DPEVKLIGVGVEGSGWADWNAIVTRTAAAELDYLSVHVYQDAPPE"
BASE COUNT          199 a          473 c          433 g          209 t
ORIGIN      
        1 gccgtggcgc gacgccggcc gcgccctggc cttcttccgc ccgaggtatg tgggcctcat
       61 gttcaagact ttcggcaacg gcccgtcagt agtcaccgat gtcgactcgc tgagctggac
      121 gaccaagccg gtcgacgccg ccccgatcag cgctcgcgtt gccgtcgact ccgcgcagcc
      181 cggcactccg ctggatcgca acctcttcgg ccacttcacc gagcacctgg gccgctgcat
      241 ctaccatgac ggcatttggg ccgagctcgt ccgcaaccga aagttcgtgg gcgacgccgg
      301 gcccgatggt gctctcgctt cgtggcggcg tttcggctcc gacgacggcg tcgaactcgc
      361 ccgcgacaac atgacctact acaccggctc tcagtcccag cgcatcacca gccggcaggc
      421 cgccggtgag catggcatcg cgcaggatgg cttccggctg cggccggtcg actatcagtg
      481 ccgcatcgtg ctccgccaga ccggcgacga cgcgccggtg acggtcagcc tgaggcgtgg
      541 cgaccaggtt ctcgccgaga gccgcatcga tcggccggcc gataagtggc gcacgtacac
      601 gtctaccctg cagcccgagt ctccggggcc cgcgtcgctg tgcatcacta ctgaggccgc
      661 tcgcggcaac ctgtggatcg gcgcgatctc gctcatgccg ggcgacgccg tggacggcat
      721 gcgcgccgat ctgatcgaag ccatccgcgc cctgcaccca cccatcatcc gttggcccgg
      781 cgggaacttc gcctcgggct accactggcg cgatgggatc ggcgagcgcg acctccgccc
      841 cgcgcgtttc gaccgcgcgt ggaacgtctg ggacaccaac gactttggca cccgcgagtt
      901 cctgcgcttc tgtgagctcg tgggctgtgc gccctacatc tgcctcaacc tgggcgaggg
      961 aacgcccgat gaggccgtcg aatgggtcca gttctgcaat ggcgccgcca acacgcccgg
     1021 cgggaggctc cgggacgatg gcgggccgcg ggagccctac ggcgtgcgct actgggggct
     1081 cgggaacgag ctctatggcg actggcagct cgggcacctc acgcccacga actacgggcg
     1141 taaagccgcc gagttcgccc gcgccgtgcg cgaggtcgat cccgaggtga agctgatcgg
     1201 cgtcggtgtc gaggggagtg gctgggccga ctggaacgcc atcgtcacgc gcaccgcggc
     1261 ggccgagctc gactacctat cggtccacgt ctatcaggat gccccacccg agaa
//
LOCUS       DAOSPL010001488         1312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_103118_length_1312_cov_3.935561, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001488 DAOSPL010000000
VERSION     DAOSPL010001488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103118_length_1312_cov_3.935561"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PLQ54_20845"
     CDS             <1..873
                     /locus_tag="PLQ54_20845"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOS95768.1"
                     /translation="VGLRRARLMVYTNSAFLHFERHLRESLAGVPAVDWPDYQAHGAR
                     LLDFPGLPQTFHGSWLDWTNARAIARALADALPGLGVDSALVQARLAQFEHEVSALEA
                     TSAALVEAAGLKGAAFVVADACLVYTVSNMGYRVGSVLMTEGAGALSGRQLAQVVERL
                     KSGELAGVVCPDSMRDSKLGEVSRQVAHDGHSRVIYARFLQPTAGASSYLSEAYYNVA
                     ALTAAAPEPARSASAAVPPGCPWRLAGAVLAAALVAIGWLAARLRSCRRGRAEVAGAG
                     IFEGPRWDDWRHRR"
     gene            851..>1312
                     /locus_tag="PLQ54_20850"
     CDS             851..>1312
                     /locus_tag="PLQ54_20850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOS95769.1"
                     /translation="MTGDIVVEAVGLGYDYVSPPVCALRGVSFELRRGAFLAVMGPNG
                     SGKSTLVRAVLGLLRPTRGSIRVAGREAWEYRREIQRIIGYVPQHDSVNQHLPMCPRD
                     IVGLAASTRGVGRGGHGNLTARVNDALAMVGMADLAHRPFGSLSGGQQQRVA"
BASE COUNT          162 a          430 c          489 g          231 t
ORIGIN      
        1 gtaggcttgc gccgggcgcg gttgatggtc tacacgaaca gtgcctttct gcacttcgag
       61 aggcacctgc gcgaatcgct ggccggcgtg cccgccgtgg actggcccga ctaccaggca
      121 cacggtgccc gcctgctcga tttccccggc ctgccgcaga cgttccacgg gtcttggctg
      181 gactggacga atgcccgggc catcgcgcgc gcactcgccg acgcgctacc gggcctgggc
      241 gtggactccg ccctcgtcca ggctcggctc gcccagttcg agcacgaggt gtcggcgctc
      301 gaggccacgt ctgccgcgct ggtcgaggcc gctggactca agggagccgc cttcgttgtc
      361 gctgacgcct gtctggtcta cacggtgtcg aacatggggt accgcgtggg cagcgtgctg
      421 atgactgagg gggcgggtgc gctgagcggg cgccagctcg cgcaggtggt ggagcgtctc
      481 aaatcgggtg agctcgccgg tgtggtttgc ccggacagca tgagagactc gaagctgggt
      541 gaggtctcgc gccaggtcgc gcacgatggt cactcgcggg tgatctatgc ccgcttcctc
      601 cagcccactg cgggggcctc gtcctatctc tccgaggcct actacaatgt ggccgccctg
      661 acggcggccg cgcctgagcc cgcgaggagt gcgagcgctg cggttcctcc gggttgtccg
      721 tggcgcctgg caggcgcggt tctcgcggcg gccctggtcg ccatcgggtg gctggccgcc
      781 cggttgcggt cgtgtcggcg gggccgcgcc gaggtggccg gggcgggtat cttcgagggg
      841 ccgagatggg atgactggcg acatcgtcgt tgaggccgtg gggcttggct atgactacgt
      901 cagcccgccg gtttgtgccc tccgtggggt ctcatttgag ctgcggcgcg gcgcattcct
      961 cgcggtcatg gggccgaacg ggtcggggaa gagcactctc gtgcgggccg ttctgggcct
     1021 gctccggccc acgcgaggga gcatccgagt cgccgggcgc gaggcctggg agtaccggcg
     1081 tgagattcag cggatcatcg gctacgttcc gcaacacgat tcggtgaacc agcatctccc
     1141 catgtgtcca cgcgacatcg tgggtctcgc tgccagcacg cgcggcgtcg gccgcggcgg
     1201 ccacggcaac ctgacagccc gcgtcaatga cgccctggcc atggtcggta tggcggacct
     1261 ggcccaccgg ccgttcgggt cgctgagcgg gggccagcag caacgggtgg cc
//
LOCUS       DAOSPL010001489         1312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_103153_length_1312_cov_2.714399, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001489 DAOSPL010000000
VERSION     DAOSPL010001489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103153_length_1312_cov_2.714399"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1312)
                     /locus_tag="PLQ54_20855"
     CDS             complement(<1..>1312)
                     /locus_tag="PLQ54_20855"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOS95770.1"
                     /translation="VITDAEGHAEASVIPRAEGSYEVRCAATDARGNRVATSQFIWVT
                     GSDSAEFAYRYPKIEIIADKKVYQPGDTARLIVNVDRPTPCALFSVEGERLNELRQVS
                     LAKKTTVLSVPITRQHIPNIYVAVCYVAGKEFVSQTKPLRVSPAHKSIRVAVSSDKPR
                     YRPGDRATFEVTTTDNHGEPVAAEVSLGVVDESIYAIREEPGPDILRFFYDRQENRVD
                     TSYSFPRVYLNPGEKSGTLVATRKYFPDTAFWAPTLVTDSAGKARVTLTVPDTLTTWR
                     ATAKAQTLSTAVGQATHQVIASRPLMARLEMPRFLRQNDEVTLRGIVHNESGRDQPVA
                     AELVLDGLETRAQAKQQARIPSAMSHVFEWLVRAPLFGNPLTTLKAVGTEDSDAMQLG
                     IPVLPHGVPRIVGDSGSTDGSVTVQLDSDTHRVDGTTALSLRLTP"
BASE COUNT          196 a          427 c          460 g          229 t
ORIGIN      
        1 ggggtgagcc ggagtgacag agccgtcgtg ccgtcgacgc ggtgggtgtc cgagtcgagc
       61 tgcacggtga cgctgccatc cgtcgacccc gagtcgccga cgatccgtgg cacaccgtgt
      121 ggcaacacgg ggatgcccag ctgcatggcg tccgagtctt cagtccccac ggccttgagc
      181 gtcgtgagcg ggttcccgaa cagcggggcc cgcacgagcc actcgaacac atgggacatg
      241 gcactgggga tccgcgcctg ctgcttggcc tgggcgcggg tctccaggcc gtcgagcacc
      301 agctcggcgg cgaccggttg gtcgcggccg ctctcgttgt gcacgatacc ccggagtgtg
      361 acctcgtcat tctggcgcag gaagcgaggc atctcgagcc gggccatgag cggccggctc
      421 gcgatcacct ggtgggtggc ctggcccaca gccgtcgaca gtgtctgcgc cttcgccgtc
      481 gcgcgccagg tggtgagcgt atcgggcacc gtgagcgtca cgcgagcctt gcccgcggag
      541 tcggtcacca gcgtgggcgc ccagaacgcg gtgtcgggga agtacttgcg cgtggcaacg
      601 agcgtgcccg acttctcgcc ggggttcagg tacacccgcg ggaatgagta actggtatcg
      661 acccggttct cctggcgatc gtagaagaac cgcaagatgt cgggtccggg ctcctcgcgg
      721 atcgcataga tggactcatc gaccacaccc agcgacactt cagcggcgac gggctccccg
      781 tggttgtcgg tggtggtcac ctcgaaggtg gcccggtccc ccggccggta gcgtggcttg
      841 tcggagctga cggcgacccg tatcgacttg tgggccggcg agacgcgcag cggcttggtc
      901 tgtgacacga actccttccc cgccacatag cacacggcca cgtagatgtt cgggatgtgc
      961 tggcgcgtga tggggaccga cagcaccgtg gtcttcttgg ccaggctgac ctgccgcagc
     1021 tcgttgagcc gctcgccctc cacgctgaac agagcacacg gcgtgggccg gtcgacgttg
     1081 acaatgagcc gggccgtgtc cccgggctgg tacaccttct tgtcggcgat gatctcgatc
     1141 ttcgggtacc ggtaggcgaa ctcggcactg tcggagcccg tgacccagat gaactggctg
     1201 gtggcgaccc gattgcctcg cgcatcggtg gccgcgcagc gcacctcgta ggagccttcg
     1261 gcgcggggga tcaccgatgc ctcggcgtgc ccctcagcgt ccgtaatgac tt
//
LOCUS       DAOSPL010001490         1310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_103380_length_1310_cov_3.032669, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001490 DAOSPL010000000
VERSION     DAOSPL010001490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103380_length_1310_cov_3.032669"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PLQ54_20860"
     CDS             complement(<1..387)
                     /locus_tag="PLQ54_20860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95771.1"
                     /translation="MLAAIAALGFLSGGYVYSRTTPVALTLAGLAVAGVWLARGPVRV
                     PRAYLVSLAAMGAFVLWIGLSILWSTAPDLSWRAFNVAALYLLVMLVCGILPGGKVQL
                     RLAAYGFASVVVVVAVLERVRRGLGRL"
     gene            628..1173
                     /locus_tag="PLQ54_20865"
     CDS             628..1173
                     /locus_tag="PLQ54_20865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95772.1"
                     /translation="MDIVRRGLVITLAAVFAACAWVPVTQASSARSILDDAMDGRVDG
                     TYSASQVRAAIKLARSDPFFSQYSDVDGVLADYLAGLNPPRTDAGGTSPVSAGQTVVT
                     PTPKAVVPVAGDSPRGDSKPHPSARPGGGRRDVAVPLPSPLLVDAGWDQAGVRLAAIP
                     WFFVVIGGASVGSMVLLRRRR"
BASE COUNT          241 a          451 c          443 g          175 t
ORIGIN      
        1 gagccggccc aggccgcgcc ggacccgctc gaggaccgcg accacgacga ccaccgacgc
       61 gaaaccgtag gcggcgagac ggagctgcac cttgccgccg ggaagtatgc cgcacacgag
      121 catcaccaac aggtagaggg cggcgacgtt gaaggcccgc caagagaggt cgggggccgt
      181 cgaccagaga atggagaggc cgatccacag cacgaaggcg cccattgcgg ccaagctcac
      241 gagataggcg cgaggcacac ggacaggacc ccgcgccagc cagacaccgg ccaccgccag
      301 ccctgccaaa gtgagcgcca cgggcgtggt ccgtgagtag acgtagccac ccgagaggaa
      361 gccgagcgcc gcgatcgccg ccagcacggc aaggatcggc agaagggagg gcagcgacgc
      421 acgggtcgcg tccgcttgtg accgtgcatg cgctggctgc aagaaccaag cccctttctt
      481 cgatacccgg cgccgcgccg gacggaccgt gcgaagtata gccgactccc gctcagccga
      541 acggctgtga ttccgatgag gcgaatgata taaggcgcat ggaacctcat cggcccatac
      601 gcggagctca gcgaaggggg tgcggagatg gacatcgttc ggcgcggcct cgtcatcacg
      661 cttgcggctg tgttcgccgc gtgcgcttgg gtgcctgtca cgcaggcgag ttcggcgcgg
      721 agcatcctcg acgacgccat ggacgggagg gtggacggca catactcggc ctcccaagtg
      781 cgcgccgcga tcaagctcgc gcggagcgac ccgttcttct cgcagtacag tgacgtcgac
      841 ggcgtgctgg ccgactatct ggccggtctt aatccgccca ggaccgacgc cggtggcact
      901 tcccctgtgt cggcagggca gaccgtagtg acacccacac ccaaggccgt tgtccccgtc
      961 gccggtgact ccccacgcgg tgacagcaag ccgcatccaa gtgcacgccc tggaggcggg
     1021 agacgcgacg ttgccgtccc cctacccagc ccgctgttgg tcgatgctgg ctgggaccag
     1081 gccggagtga gactcgcggc catcccctgg ttcttcgttg tcattggcgg cgcatcggtc
     1141 ggcagcatgg ttctcctgcg gcggcgccgt tgaccgcagg cgagtcgggc ctccactccc
     1201 cgactgcgcg ctgcgccgac gggcacgacg tcgcagcctc acggctgaca cccggaaccc
     1261 gattgtcgac agaggccgcc cgcatggaga cacgggacca tcacgtgcac
//
LOCUS       DAOSPL010001491         1308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_103646_length_1308_cov_2.799681, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001491 DAOSPL010000000
VERSION     DAOSPL010001491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103646_length_1308_cov_2.799681"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="PLQ54_20870"
     CDS             <1..1125
                     /locus_tag="PLQ54_20870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-agarase"
                     /protein_id="HOS95773.1"
                     /translation="GRGLHGYYAGRDVRTYSFTMGNLVRKYGADWREVFSDVCHRRLR
                     SWGMNTIGNWSDWDLFQQDRTPYVVPVGFGGKMLEGSQGYWGKFRDVFDPSFTEAVRD
                     GMRGAAAVSADDPWCIGYFVDNEIAWGDDTSLAVAALVSPPDQAAKQEFVRDLRAKYG
                     DVAKLNEVWGTDYASWDALLAADEPPSPRRARQDLTAFYTKSAERYFSTVRAAVDELA
                     PHHLYLGCRFAGANELAARAAAKYCHVVSYNLYQRSVADFRLPEGVDAPLIIGEFHFG
                     ALDRGMFHTGLVPVESQEARAESYRSYVQGCLRNASFVGCHWFQYFDEPTTGRWLDEE
                     NYQIGFVDTADTPYPETIAACRDVGYSLYEYRREVRATRP"
BASE COUNT          212 a          439 c          445 g          212 t
ORIGIN      
        1 gggcgcgggc tgcacggcta ctacgcgggg cgagatgtgc gcacctactc tttcaccatg
       61 ggcaacctgg tgcgcaagta cggtgccgac tggcgggagg tcttctccga cgtatgccat
      121 cgacgcctgc ggagctgggg catgaacacc atcggcaact ggtccgactg ggatctcttc
      181 cagcaggatc gcacacccta cgtcgtgccg gtgggcttcg gcgggaagat gctcgagggc
      241 agccagggct actggggcaa gttccgcgac gtgtttgatc cgagcttcac tgaggccgtg
      301 cgcgatggta tgagaggtgc ggccgcggtg tcggccgatg atccctggtg tatcggctac
      361 tttgtcgaca acgagatcgc gtggggcgac gacacgtcgc tggccgtcgc ggccctcgtc
      421 tcgcctccgg accaggccgc gaagcaggag ttcgtccgcg acctccgggc caagtacgga
      481 gacgtggcca agctcaacga ggtctggggc accgactacg cctcgtggga cgcgctcctt
      541 gccgccgacg agccaccaag cccccgcagg gcgcgccagg acctaacggc tttctacacc
      601 aagtcggcgg agcggtactt ctcgaccgtt cgcgcggcgg tcgatgagct cgcgccgcac
      661 catctgtacc tcgggtgccg gttcgccggc gccaacgagc tcgccgcacg ggccgcggcg
      721 aagtactgtc acgtggtcag ctacaacctg taccagcggt cggtggcgga cttcaggctg
      781 cccgagggcg tcgacgctcc cctgatcatc ggcgagttcc acttcggtgc gctggaccgc
      841 ggcatgttcc acacgggctt ggtgcccgtc gagagtcagg aggcccgggc cgagtcctac
      901 cgcagctatg tgcagggctg cctccggaat gcaagcttcg tcggctgcca ctggttccag
      961 tacttcgacg agcccacaac gggccgctgg ctcgatgagg agaactacca aatcggcttc
     1021 gtcgacaccg cggacacacc gtatccggag accatcgccg cctgtcgcga cgtcgggtac
     1081 tcgctctacg agtaccggcg ggaggtgcgg gcgaccaggc catagccgcg ggcgggtcgg
     1141 tggcccgatg atggctgcag gacccgcccg acgactggcc gaacgaaccc acgccatgcg
     1201 cacctcggcg acaaccctaa ctgagcccac ccgcgagatc ccgatccgcc acacggccga
     1261 cgtcgtcgtc tgcggcgggg gtacggcggg cattgcggcg gcctgctg
//
LOCUS       DAOSPL010001492         1304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_104091_length_1304_cov_3.347478, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001492 DAOSPL010000000
VERSION     DAOSPL010001492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104091_length_1304_cov_3.347478"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PLQ54_20875"
     CDS             <1..195
                     /locus_tag="PLQ54_20875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95774.1"
                     /translation="VEDRVYKFELATLARQGVVKLEDAPKLGLPVMVANGGSLFVLKD
                     NRLYEVDPGALTVRGRVDAD"
     gene            380..>1304
                     /locus_tag="PLQ54_20880"
     CDS             380..>1304
                     /locus_tag="PLQ54_20880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95775.1"
                     /translation="MTRSLRHSQPLLLMAWLLGTLAPMASPAAAEGARPEDHRSLNHC
                     GICCALRGGACRDRFVIPPYAIAHACPSPTHYCPGPGERDGSWEPQTSGAGAATGGAV
                     AEGPGVVPSYVPPPTRAVSSIEVPVPSYVPQAARPDPESGPALLTSPTGIPVVSGGDV
                     PVTGVIAPQTLANPDQAQFAHSPGDCVLYTGPFILERVPVLAEHKFPRYFACSMRVIP
                     KLRLYPFLAFNVYVPHEGNRVLLATNFVFNSLPVTNSDVVDVGPTQGLGYLEQYVEGG
                     IEKYMILRLRDGTVKVTRLDAEGRGVYSVYSI"
BASE COUNT          203 a          419 c          424 g          258 t
ORIGIN      
        1 gtcgaggatc gtgtctacaa gttcgagctg gccactctcg cccgccaagg cgtggtgaag
       61 ctcgaggacg cgccgaagct cggccttcct gtcatggtcg ccaacggagg gtccctgttc
      121 gttctgaagg acaaccgcct ctatgaggtc gatccgggtg cgctcaccgt aaggggtcgg
      181 gtggatgccg actagggcgt gccagtcgcg tcgcggtttc gccgccggag ggacgaacct
      241 gccggcctct gaataagtca atcggtgtga gacggtaatg gcgctcgctt cgatgtgctt
      301 gggatgcgcg acgtgcttgc agcttgtgct tgcgtgagtc ttccaatctt cgccgagtat
      361 caaggaacga tgtcccgaca tgacgcgatc actgcggcac tcccagccac tcttgctcat
      421 ggcctggttg cttggaaccc tggcacccat ggcgtcgccc gcggcggcag agggtgctcg
      481 gccggaggat caccgtagcc tcaaccactg cgggatctgc tgtgcgctac gcgggggggc
      541 ctgccgggac cggttcgtga tcccgcccta tgccatcgcc cacgcctgcc ccagccccac
      601 ccactactgc ccgggtcccg gagaacggga cggctcctgg gaaccccaaa ccagtggggc
      661 gggcgccgcg accggagggg ccgtcgcgga ggggcctggc gtggttccgt cctatgtccc
      721 gccccccacg cgcgccgtgt cgtcaatcga ggtgcctgtg cccagctacg tgccccaggc
      781 tgcgcggccc gatccggaga gtgggccggc gctgttgacc tcgcccacgg ggatcccggt
      841 cgtcagtggc ggcgatgtgc cggtcacggg tgtgatcgcg cctcagaccc ttgccaatcc
      901 cgaccaggcg cagttcgccc actcaccagg agactgcgtc ctgtacacgg gtcctttcat
      961 actcgagcgc gttcctgttc tcgcggagca caagttcccg cgctactttg catgctcgat
     1021 gcgtgtcata cccaagctgc gtctgtatcc gttcctggcg ttcaacgtct atgttcccca
     1081 cgagggtaac cgggtgctgt tggcaacgaa ctttgtcttc aatagcttgc cagtcaccaa
     1141 cagcgatgtt gtcgatgtgg gccccaccca gggcttgggt tacctcgagc agtacgtcga
     1201 gggcggaata gagaagtaca tgatcctgcg tctccgggac ggcacggtga aggtgacgcg
     1261 gctggatgcc gaggggcgtg gcgtatactc ggtctactcg atct
//
LOCUS       DAOSPL010001493         1301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_104456_length_1301_cov_2.557785, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001493 DAOSPL010000000
VERSION     DAOSPL010001493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104456_length_1301_cov_2.557785"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLQ54_20885"
     CDS             <1..540
                     /locus_tag="PLQ54_20885"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOS95776.1"
                     /translation="APSTADDVKGLLKAAVRDDNCVVFIEHQNLYTEKGVCPEEEYTI
                     PLGQGIVRREGTDITVVAYSYMAPVALQAAEMVAGDGISVEMIDPRTLRPLDVDLIAG
                     SVSKTGRLLVVCQAPKFGCFGETIVYTTQERCFSDMKAPARIVAAYDVPPPMAAPLER
                     ENLPTPEKVAQAIRELCGR"
     gene            537..>1301
                     /locus_tag="PLQ54_20890"
     CDS             537..>1301
                     /locus_tag="PLQ54_20890"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HOS95777.1"
                     /translation="MTLVQIGAGNIGRSFVGQLFARAGYEVVFIDVVDEVVAALNERR
                     CYTVEIRDEHPESIQVRNVRAIHGRDMEAAAGALAAADIAATAVGPGALRFIYPTIAR
                     ALVLRNERGAAPLDIIICENLRGAARAFAEGLKPLLPEGFPLADSVGLVETSIGKMVP
                     IMTEEQRRTDPLLVYAEAYNTLICDACAFRNPIPEVPGLLPKANMRAYVDRKLFVHNL
                     GHALTAYLGHVRAPGAVFVWQAVEDERVGPSVRAGMW"
BASE COUNT          210 a          425 c          438 g          228 t
ORIGIN      
        1 gcgccctcaa ccgccgacga tgtcaagggg ctgctgaagg cggccgtgcg agacgacaac
       61 tgcgtggtct tcatcgagca ccagaacctc tacaccgaga aaggcgtctg ccccgaggag
      121 gagtacacca ttcctctggg ccaaggcatc gtccgccgcg aggggaccga catcacggtc
      181 gtggcctaca gctacatggc gccggtggcc ctgcaggcgg ccgaaatggt ggcgggagac
      241 ggcatatcgg tggagatgat cgacccgcgg accctgcggc cactcgatgt ggacctcatc
      301 gccggttcgg tgtccaagac ggggcgcttg ctggtggtct gccaggcgcc caagttcggg
      361 tgttttggtg agaccatcgt ctacaccact caagagcgct gcttcagcga catgaaagcg
      421 ccggcgagga tcgtcgcggc ttacgatgtg ccgcctccca tggccgcgcc cctcgagaga
      481 gagaacctgc cgactccgga gaaggtcgcc caggcgatcc gggagctctg cgggcgatga
      541 ccctggttca gatcggcgcg ggcaatatcg gccggtcctt tgtgggccag cttttcgccc
      601 gcgccgggta cgaggtcgtg ttcatcgatg tcgtcgatga ggtggtcgct gctctgaatg
      661 agcggcgatg ctacacggtc gagatccgcg acgagcaccc cgaatcgatc caggttcgaa
      721 atgtccgcgc gatccatggc cgggacatgg aggcggcggc cggcgcactg gccgccgccg
      781 acatcgccgc cacggcagtg gggcccgggg cactccggtt catctacccc accatcgctc
      841 gggcactggt gctgcggaac gagcgcggcg ccgctccgct ggatatcatc atctgcgaga
      901 acctgcgcgg cgcggcccgg gcattcgccg aggggctcaa gccgctcttg ccggaggggt
      961 tccccttggc cgactcggtc ggcctcgtcg agacctccat cggcaagatg gtccccatca
     1021 tgactgagga gcagcggcgg accgatcccc ttctggtcta tgccgaggcc tacaacacgc
     1081 tgatctgtga cgcctgtgcc ttccgcaacc cgattcccga ggtgcccggg ctcctgccca
     1141 aggccaacat gcgcgcctat gtggatcgga agctctttgt gcacaacctg ggccacgcgc
     1201 tcaccgcgta tctcggccac gttcgggctc ccggggccgt gttcgtgtgg caggctgtcg
     1261 aggacgagcg cgtgggtcct tcggttcgtg ctggcatgtg g
//
LOCUS       DAOSPL010001494         1299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_104726_length_1299_cov_2.416399, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001494 DAOSPL010000000
VERSION     DAOSPL010001494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104726_length_1299_cov_2.416399"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PLQ54_20895"
     CDS             <1..268
                     /locus_tag="PLQ54_20895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95778.1"
                     /translation="VQAAGCRLIVTVLPHAYTTETYGAGTFEGFLCQLGDWCREHDAG
                     FVNLSSMVPREAFGDAVHFDREYIPLVCERLAQAVASDLRGRRR"
     gene            265..1296
                     /locus_tag="PLQ54_20900"
     CDS             265..1296
                     /locus_tag="PLQ54_20900"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOS95779.1"
                     /translation="MSLAQLKLAARILLKTAEGALHLAYVAGVIASFWRWRPWQLLVH
                     FALLIVVIARQLNPMDAVTSSESVLGTHLILPVLLAVWTHCALRVAARFPGHKQLILW
                     STMAGLLALWMATKVGALRTAVPPLGDLLGPAGLSVFGLSYILVKLLHVLADSAGPRP
                     PEVRLTTLLGFTCFAPTFLCGPMHRYDEFASSFDHLPSAWNRDWATVIRRVTWGTFKL
                     MVLAGALGEGVLPLLAHPAEQSAGVLWLAVYGYSAYIYLNFSGVSDLAIGLGAAFGVV
                     VPENFDRPYLQLDIQSFWRTWHMTFTRWLTAYVFMPITKRLMRTSLRKQPKVAASIGY
                     VLTFTFCGL"
BASE COUNT          174 a          435 c          430 g          260 t
ORIGIN      
        1 cgtgcaggcg gcggggtgcc gcctcatcgt gaccgttctg ccgcacgcct acacgacgga
       61 aacctatggc gccgggacgt tcgaggggtt cctctgccaa cttggcgact ggtgccgaga
      121 gcacgacgcc gggttcgtca acctgtcctc catggtgccg cgcgaagcgt tcggcgacgc
      181 ggttcacttc gaccgcgagt acatcccgct ggtctgcgag cggttggctc aggcagtcgc
      241 atccgatctg cgagggcgcc ggaggtgagc ctggcgcaac tgaagctggc cgcgcggatc
      301 ctcctgaaga ccgccgaggg agcactccac ctcgcgtatg ttgccggcgt gatcgcctca
      361 ttctggcggt ggcggccctg gcagctcctc gtgcactttg cgctgctgat cgtcgtcatc
      421 gcgcggcagc tcaacccgat ggacgcggtg acctcgagcg agtcggtgct cgggacgcac
      481 ctgatcctgc ccgtgctctt ggccgtgtgg acccactgcg cgctccgtgt cgccgcgcgg
      541 ttcccgggcc acaagcagct catcctctgg tcgaccatgg ccggccttct ggcgctctgg
      601 atggcgacca aggtcggtgc cctgaggacg gcggtgccgc cactgggcga tctgctgggc
      661 ccggcaggac tgtcggtgtt cgggctgtcg tacatcctgg tgaagctcct ccatgtgctg
      721 gccgactccg ctgggccgcg gccgccggag gtgcgcctga ccactctgct gggcttcact
      781 tgcttcgccc ccacattcct gtgcggaccc atgcaccgct atgacgagtt cgcttcgtcg
      841 tttgaccacc tcccctctgc gtggaatcgc gactgggcaa ccgtgatccg ccgcgtgacc
      901 tgggggacgt tcaagctgat ggtgctagcc ggggccctgg gcgagggcgt cctgcccctg
      961 ttggcccatc cggcggagca gagcgccggt gtactgtggc tggcagtcta tggctacagc
     1021 gcctacatct acctgaactt ctcgggcgtc tcggatctgg ccatcgggct cggggcggcg
     1081 ttcggcgtcg tggtgccgga gaacttcgac cggccgtacc tccagctcga catccagtcg
     1141 ttctggcgga cgtggcacat gacgttcact cgatggctga cggcctatgt gttcatgccc
     1201 atcacgaagc gcctgatgcg gacttcgttg cgcaagcagc ccaaggtggc cgcttcgatc
     1261 ggctacgtac tgacgttcac attctgcggc ctgtagcac
//
LOCUS       DAOSPL010001495         1298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_104803_length_1298_cov_2.753017, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001495 DAOSPL010000000
VERSION     DAOSPL010001495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104803_length_1298_cov_2.753017"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            489..770
                     /locus_tag="PLQ54_20905"
     CDS             489..770
                     /locus_tag="PLQ54_20905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95780.1"
                     /translation="MIRETDDRHHPIAVHKTDGLSLPEFAGHPESGRYAIQCNVNPAD
                     ELHRGLLEMRRDAARRYAVILAEAVYWGTGAEARRKAWDRAMRALRPWQ"
BASE COUNT          210 a          423 c          472 g          193 t
ORIGIN      
        1 agcgggaggc cggcggtagt gcgtcggtgg tccggcgccg ctgtgcgagc cgggcgatcc
       61 cgagggctcc tcttgtcgcg acaaactggg gcctgacggg acccgtcccg gcgaccaacc
      121 cgggctggtc cgccagcagc agcaggagaa ggacgcccat ccggtctcca tgaccgtggt
      181 ccgatcccac agggggatgg tagcggcgcg gataccccag tcgtggacca cgacccggcc
      241 caatgagtgg gccctcagac cctggaccag tgggaccagt ggctctcggc gatagactgg
      301 gcgggggtcg gggccttcgt gttcttgtag ggcgacagca ctctggtgtg gcccacggac
      361 tggggctcga gcgcgcgttc atccgtgcgg ggttcggcca gcccaagcac cacacacccc
      421 tgatctggtg agtcgccgag gagtacgccg aggcgctcag tgccgctggc gtacgggacc
      481 tcgccgcagt gatccgcgag acggacgacc ggcatcaccc catcgccgtt cacaagaccg
      541 acgggctgtc actccctgag ttcgccgggc acccggagtc cgggcggtac gccatacagt
      601 gcaacgtcaa cccagccgat gaactacatc gagggctgtt ggagatgcgt cgcgacgcag
      661 cgcggcgcta cgcagtcatc ctcgccgagg ccgtgtactg gggcaccgga gcggaagctc
      721 ggcggaaggc ctgggatcgc gcgatgcggg cactccggcc atggcaatga gcatggatac
      781 tgccagcaca agcccagacg agcttgaggg gtgtgggctc tcgtgcactt cttcgagggc
      841 cctgggacgg gcctggagtc ccatggtgag tgggcggccg ggcgcgcgcg atacgtcctg
      901 gctatgggca cggtggcgca cacggacccc gcgatgctcc tcctgcgggc accggaagga
      961 cgagggcccg aggtcgtgct ggacctacgc agaggagagc gcggaggcgt agcaccgatc
     1021 ggcccttggg ccgtcttgct tgcgtgcatg gtctggcgaa cgtaacatca tatatgggtt
     1081 cttgtaacgc gcacgacgcg gtgcagtgtg gtggggggaa gatgagcgcg cctgaggtac
     1141 tccacacgcc cggcgggatg cagccggagt cctcgccccg gccgggcagc cggacgcccc
     1201 ccatacgccc cggctcggcc gcaggccacg cccaagcaag gctcgctgtg gccctcgcag
     1261 ggtgcctctt ggccgccatg tccgggcaag atgcatgc
//
LOCUS       DAOSPL010001496         1297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_104928_length_1297_cov_2.660225, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001496 DAOSPL010000000
VERSION     DAOSPL010001496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104928_length_1297_cov_2.660225"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PLQ54_20910"
     CDS             complement(<1..537)
                     /locus_tag="PLQ54_20910"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOS95781.1"
                     /translation="MAPAPRRIVLASGSPRRVGLLRELGLRFSVVAPDVDEASLGPFA
                     DPPAAVEALAYAKASRVAAGCRDGLVLGFDTAVVIGDVVLGKPTDTADAVRMIQCLAG
                     QEHEVLTGLCVLDVSSARLPGCDGLTPPGLAADGEEYGRAWMAGPGVAVGYERSFVRF
                     GPLSPEEARAYVALGESLD"
     gene            complement(537..>1297)
                     /locus_tag="PLQ54_20915"
     CDS             complement(537..>1297)
                     /locus_tag="PLQ54_20915"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOS95782.1"
                     /translation="FCQNAEISQAIRGGRAVTAEGLVNLARDEPGNAGISYTYNEPLI
                     NYEFVRDCAALAHERNLANVLVSNGYIRPEPFERLAPLIDAMNLDIKSMADDFYVKLC
                     GARVAPVLETARRAKTHMHLEITNLVIPGENDSDDDLRGLAQWVSENLGRDTPVHYTR
                     YHPAYRMTTPATPDSTLLRARELAGPYLDFIYLGNTMLPGTSDTRCPHCGALAVTRSG
                     FRARIVGLDADGHCGGCGAALPLVLGHGGRWAAR"
BASE COUNT          229 a          445 c          426 g          197 t
ORIGIN      
        1 gtcaagcgac tccccaagtg ccacatacgc tcgggcttcc tcgggcgaga ggggtccaaa
       61 gcgaacgaaa gagcgctcat atcccacagc cacgccgggg cccgccatcc aggcccggcc
      121 gtactcctca ccatcagcgg caaggccggg cggcgtcagg ccgtcgcaac caggcagtcg
      181 ggccgaagac acatccagga cacacaggcc cgtgagcacc tcatgttcct gtccggccag
      241 acactggatc atccgcacgg cgtccgcggt gtcggtgggc ttcccgagga ccacgtcgcc
      301 gatcacgacg gccgtgtcga acccgagcac cagaccatcg cggcacccgg cggccacccg
      361 cgaggccttc gcataggcga gcgcctccac ggcggccgga ggatcggcga aggggccgag
      421 cgaggcctcg tccacatcgg gtgcaacgac ggagaagcgc agccccagct cccggaggag
      481 ccccacccgt ctcggtgacc ccgaagcgag aacgatccgg cgaggggcag gagccatcag
      541 cgggccgccc agcgtccgcc gtggccgagg accaggggca gggccgcacc acagcccccg
      601 cagtgcccgt ccgcgtccag tcccacgatc cgcgcccgga accccgagcg ggtcacggcc
      661 agagctccac agtgcggaca ccgtgtgtcg gatgtgccgg gaagcatcgt gttacccagg
      721 tagatgaagt ccaggtaggg cccggccagc tctcgagctc tgagcagcgt cgaatctggt
      781 gtcgccggtg tggtcattcg gtatgccggg tggtagcgcg tgtagtgcac gggcgtgtct
      841 cgcccgaggt tttcactcac ccactgcgcg agtcctcgca ggtcgtcgtc actatcgttc
      901 tcgccgggaa tcacgaggtt ggtgatctcg aggtgcatgt gcgtcttggc ccgccgtgcg
      961 gtctcgagaa ccggagccac cctcgcgccg cacagtttca cgtagaagtc atcggccatg
     1021 gacttgatgt ccaggttcat cgcatcgatc agtggcgcga gccgctcgaa tggctcgggc
     1081 cggatgtagc cgttcgagac aagcacattc gccaagttgc gctcgtgcgc gagggcggca
     1141 caatcgcgaa cgaactcata gttgatcagg ggctcgttgt acgtgtacga gataccggca
     1201 ttgccgggct cgtcccgggc caggttgacc aatccctcgg ccgtgacggc gcgccccccg
     1261 cgtatggcct ggctgatctc ggcgttctga cagaacc
//
LOCUS       DAOSPL010001497         1295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_105168_length_1295_cov_3.272581, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001497 DAOSPL010000000
VERSION     DAOSPL010001497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105168_length_1295_cov_3.272581"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PLQ54_20920"
     CDS             complement(<1..521)
                     /locus_tag="PLQ54_20920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95783.1"
                     /translation="MSVGCPARVHALGLGLCGMFCALLGVPPALAGGALRPMQLRCEY
                     LVAPLGIATDTPRLSWVCQSTRPAARGLSQTAYQVVAASTREALDREPDLWDTGRVDS
                     SDPLGIQYAGKPLSSRQQVWWKVRLWDQDGQSSDWSRPATWTMGIIGNEPWHAQWIAG
                     VQDGTAMPIFRRE"
     gene            complement(632..>1295)
                     /locus_tag="PLQ54_20925"
     CDS             complement(632..>1295)
                     /locus_tag="PLQ54_20925"
                     /inference="COORDINATES: protein motif:HMM:NF014886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOS95784.1"
                     /translation="PVLQKRIIAAANRQAIPVITATHMLESMIQNPRPTRAEASDVAN
                     AIFDGTDAIMLSGEASIGRYPVEAVTMMDRIAREAEKSLLAARPFIDAPPRPGNDYPD
                     ALARAACQVAVDLNASGIVAMTRSGYTARLVSAYRPSVPIIAVTPSLATCERAGLYWG
                     VYPYLCAEFSAGDDMVAAVNEAAVTVAGVAPGSTVVVISGAQVLDSSTTHALRLQRIS
                     GR"
BASE COUNT          207 a          417 c          487 g          184 t
ORIGIN      
        1 aactcgcgcc ggaagatggg catagcggtg ccgtcctgga ccccggcgat ccactgggcg
       61 tgccagggct cgttgccgat gattcccatc gtccaggtgg cgggacggct ccagtcggag
      121 gactggccgt cctggtccca gagccgaacc ttccaccata cctgctggcg ggacgagagg
      181 ggcttgcccg cgtactggat gccgagtggg tcgctcgagt ccacgcggcc ggtatcccag
      241 aggtcgggct cgcggtcgag cgcctctcgc gtcgaggccg caacgacctg gtaggccgtc
      301 tggctcagcc cacgggcggc cggtcgagtg gactggcaca cccagctcag cctgggcgta
      361 tcggtggcga tgcccagggg cgcgacgagg tactcgcagc ggagctgcat gggtcgcagc
      421 gcaccaccgg cgagcgccgg gggcactccc agcagagcgc agaacatgcc gcagaggccg
      481 agccccaacg cgtgaactcg agcgggacag ccgacagaca cgcggtcatc atcctccaag
      541 cagacgtgcg gccggcgcag agccggccac cgagggcatg atacgggccg ggccgccatg
      601 tggccagagg tagcgtgtcg ccccacgcgg cctagcgtcc cgagatgcgc tgcagcctca
      661 gggcgtgcgt ggtgctgctg tccagtacct gggcaccgga gatgaccacc accgtgctgc
      721 cgggcgcgac gccggccacc gtgaccgcgg cctcattgac ggccgcgacc atgtcgtcgc
      781 ccgcggagaa ctcggcgcac aggtaggggt agacacccca gtagaggccg gcgcgctcac
      841 atgtggcgag actgggcgtc accgcaatga tggggacact cgggcggtac gcggacacca
      901 ggcgtgccgt atagccggat cgggtcatgg ccacgatgcc ggaggcgttg aggtcgaccg
      961 ccacctgaca cgcggcccgc gcgagggcat ccgggtagtc attacccggg cgggggggag
     1021 cgtcgatgaa gggccgagcg gcgaggaggc tcttctcggc ctcgcgcgcg atgcggtcca
     1081 tcatggtgac ggcctcgacc gggtacctgc cgatggaagc ttcgccggac agcatgatgg
     1141 cgtcggtgcc gtcgaagatg gcattggcga cgtccgaggc ctcggcgcgg gtcggtctcg
     1201 gattctggat catcgactcg agcatgtggg tggcggtgat gacggggatg gcctggcgat
     1261 tggcggcggc gatgatgcgc ttctggagca ccggc
//
LOCUS       DAOSPL010001498         1295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_105236_length_1295_cov_2.042742, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001498 DAOSPL010000000
VERSION     DAOSPL010001498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105236_length_1295_cov_2.042742"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="PLQ54_20930"
     CDS             complement(<1..1258)
                     /locus_tag="PLQ54_20930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOS95785.1"
                     /translation="MGYSDTLNLPKTAFPMKADLATREPDILKLWEDLDLYRLVRSHR
                     EGCPKWILHDGPPYSNGDIHIGTAQNKILKDISVKYRTMRGYDCPYVPGWDNHGLPLE
                     QVLYRETGRNWRDVGALEWRQQCHELALRYVERQGQQFRRLGVRGDWDHPYLTLRGEY
                     EAEQIRVFGEIARAGHIYRGLRPVYWCGHCETALADAEIEYTDKTSDSIYVRFPWNDD
                     RSRLSPGLSESDRVSVVIWTTTPWTLPANMAIAVHPDARYVVAFVEGEYIIVAEALLD
                     RVLELWGAPKRDIIATHAGKSLEGCLCRHPFADRSSPIVLAEYVTLEDGTGCVHTAPG
                     HGEDDFNTGRQYGLPVLSPLNDRAELTEAAGIFAGKYCEDANADIIAWLRDHGYLVQS
                     GKTLHQYPHCWRCHHPIAFRATEQWFI"
BASE COUNT          244 a          390 c          416 g          245 t
ORIGIN      
        1 tgatgaacca ctgttctgtc gcccgaaagg ctattggatg gtgacaacgc cagcaatgag
       61 ggtactggtg gagcgtcttc ccactctgga ccagatagcc atggtccctg agccatgcga
      121 tgatgtccgc gttggcgtcc tcacagtact tgcccgcgaa gatgccagca gcctccgtga
      181 gttcggcccg gtcattgagc gggctcaaga ccggcaagcc atattggcga ccggtgttga
      241 agtcatcctc accgtgaccc ggagccgtgt ggacacaccc ggtgccgtct tcgagggtaa
      301 cgtattcggc caggacgatg ggagaagagc gatcggcgaa gggatgacgg cagagacacc
      361 cttcgagtga cttacccgcg tgtgtggcga tgatgtctcg cttgggggcg ccccagagct
      421 cgagcacacg gtcgaggagg gcctcggcga cgatgatgta ctcgccctcg acgaaagcca
      481 ccacgtaacg cgcatcggga tggacggcga tggccatgtt ggccggtagg gtccacgggg
      541 tggtggtcca gatgaccacc gagacacggt cggattccga gagcccaggg gacagacgcg
      601 agcggtcgtc gttccacggg aatcggacat agatggagtc gctggtcttg tcggtatact
      661 cgatctcggc gtcggccagg gccgtctcgc agtgcccgca ccagtagacg gggcgcaggc
      721 cacgatagat gtggcccgcc cgggcgatct cgccaaacac gcgaatctgc tcggcctcat
      781 actccccccg gagcgtgagg taggggtggt cccagtcacc acgaactccc aggcggcgga
      841 actgctgccc ctggcgttcg acatagcgca gagcgagctc gtggcactgc tgccgccact
      901 cgagggctcc cacgtcgcgc cagttccgcc cggtctcgcg gtagagcacc tgctccaacg
      961 gcagaccgtg gttgtcccac ccgggcacgt aagggcagtc atagccacgc attgttctgt
     1021 acttgacgct aatgtctttg aggatcttgt tctgtgctgt gccgatgtgg atatcgccgt
     1081 tggaatacgg aggcccatca tgaaggatcc acttcgggca tccttctcga tgactgcgga
     1141 cgagccggta gaggtccagg tcctcccata gcttcaggat gtctggctcc cgagtcgcca
     1201 ggtccgcctt cattggaaaa gcggtcttcg gcaggttgag cgtgtcggag taccccattc
     1261 aaaccacccc atctagtgac gcgggacctg gaacc
//
LOCUS       DAOSPL010001499         1294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_105287_length_1294_cov_3.459241, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001499 DAOSPL010000000
VERSION     DAOSPL010001499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105287_length_1294_cov_3.459241"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1294)
                     /locus_tag="PLQ54_20935"
     CDS             complement(<1..>1294)
                     /locus_tag="PLQ54_20935"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS95786.1"
                     /translation="MASPTAWRCGVCGYIHRGDEPPEWCPVCGAYRDDFELYEEPAPA
                     PASVPTAWRCLNCGHVHGGGEPPEVCPVCAAGRDRFDAQSAPGAAADRASDLGRAIVV
                     GGGIAGLSAAEAIRAAAPEAEVLLVCREPGLPYYRLNLTRYLAGEVPAEALPIHPGAW
                     YETQAIDLVAGAEAAEIDPGTHSVRLSDGTWEAYDRLVITAGAHPFVPPIPGSRREGV
                     TALRTIQDADRILGAVGDGVRCVCLGGGILGLETAGALARRGAQVTILEGYAWLLARQ
                     LCEGAGRLLARLVGALGIDVRLGVRVQEILGDERVRSVLLEGGDEVRADLVVITTGVR
                     PNSHLARSAGLGVNQGIIVDDHMKTSAPDILAAGDVAEHRGTVYGLWAPAQFQGSIAG
                     MNAAGLSVEFGGIPRSNTLKVLGIDLFSVGLVEPQDASF"
BASE COUNT          234 a          488 c          401 g          171 t
ORIGIN      
        1 aaagctggcg tcctgaggtt caaccagacc aacgctgaac agatcaatgc ccaagacctt
       61 gagggtgttc gaccggggga tgccgccaaa ctcgaccgac aggccggccg cgttcatgcc
      121 ggcgatgctg ccctggaact gggcgggagc ccagagacca tacacagtgc cccgatgctc
      181 cgccacgtcg ccagcggcca ggatgtcggg cgcggaggtc ttcatgtgat catcgacgat
      241 aatgccctgg ttcacaccca agcccgcgga gcgcgccaga tggctgttgg gccgcacccc
      301 ggtggtgatc acaaccaggt cagcgcggac ctcgtctcca ccctccagca gcacactgcg
      361 aacccgctcg tcgcccagga tctcctgcac gcgcacaccc aggcgtacgt cgatccccag
      421 ggcaccgacc agcctcgcga ggagccgccc ggccccctca cagagctggc gggccagcag
      481 ccaggcatac ccctccagaa tcgtgacctg ggcgccccgt cgggccagcg caccggccgt
      541 ctccaacccc agaatgccgc ccccgaggca cacacaccgg acgccgtcgc ccacagcgcc
      601 gaggatacga tccgcatcct ggatcgtgcg tagagccgtc acgccctcac ggcgagaccc
      661 cggaatcggg gggacgaacg gatgggcgcc cgcggtgatc accagccggt catatgcctc
      721 ccatgttccg tcggacagcc ggactgagtg agtcccggga tcgatctccg ctgcctctgc
      781 acctgcgacc aggtcaatgg cttgagtctc ataccaggcg cccgggtgaa tgggcagcgc
      841 ctccgcaggg acttcgcccg ccagatagcg ggtcaggttg agccggtaat agggcagtcc
      901 gggctcccga cacaccaaca agacctcggc ctcgggagcg gccgcgcgga tagcttccgc
      961 tgccgatagc ccggcgatac ccccgccgac cacgatggct ctgccgagat cgctcgcccg
     1021 gtcggccgcc gctcccggcg cgctctgagc gtcgaaacgg tcgcgcccgg cggcacagac
     1081 cgggcacacc tcaggcggct cgcctccccc atggacatgc ccgcagttga ggcaccgcca
     1141 ggcggtcggc acggacgccg gcgctggcgc cggctcctcg tagagctcga agtcatcgcg
     1201 ataggcccca cacaccgggc accactcggg cggctcatca ccacgatgga tgtacccgca
     1261 gaccccacag cgccacgcgg tgggtgatgc catc
//
LOCUS       DAOSPL010001500         1294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_105316_length_1294_cov_2.473769, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001500 DAOSPL010000000
VERSION     DAOSPL010001500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105316_length_1294_cov_2.473769"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="PLQ54_20940"
     CDS             <1..1245
                     /locus_tag="PLQ54_20940"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HOS95787.1"
                     /translation="IRWHVVGALHTYLEQFIERAEARGAHVHWADDAEQARRQVLAIA
                     REHECRRVLKSKSMVTEEIGLRHALESAGMVVKETDLGELIVQWAGEAPSHLTGPAAH
                     KSRGDIGRLIAEQLGVPFTDDPRALTGLVRDHLRGDFLTADLGISGANFLIAETGSVT
                     IVENEGNARLVTGLAPVHVVVAGIERILPRLADLEVFLQLLAGSNAGQKLTAYTSVIT
                     GHRREHEVGGPEHVHVVLVDNGRSRMLADPIMRPALMCIRCAACLNVCPVYRRVGGHA
                     YGWCYPGPIGQVLGADMWSEQEGYDLAFGSTLCGACAEACPVRIDLPTVLLRLRQRAA
                     EAEAPGGAAERRRFRLWAFAMRHPRIYRMLPNWLLRLGMRVAPEIPRRVAPGWVATRD
                     LPEPAPQTFAQRWRSTGGFSHD"
BASE COUNT          192 a          416 c          470 g          216 t
ORIGIN      
        1 attcgctggc acgtggtggg cgcacttcac acttatctcg agcagttcat cgaacgggcc
       61 gaggcgcggg gggcgcatgt ccactgggcg gacgacgccg agcaggcccg ccgccaggtg
      121 ttagccatcg cgcgcgaaca cgagtgtagg cgcgtgctga agtccaaatc gatggtgacc
      181 gaagagatcg ggctccgcca cgccctcgag agcgcgggca tggtcgtcaa ggagaccgac
      241 ctgggcgagc tgatcgtcca gtgggccggc gaggcgccct cgcatctcac tgggcccgcc
      301 gcccacaaat cgcggggtga tattggacgg ctgattgccg agcagctggg cgtcccattc
      361 accgacgacc ctcgtgcgct cacgggcctg gtgcgagacc atctccgggg agacttcctg
      421 acggccgatc tgggtatcag cggggccaac ttcctcatcg ctgagaccgg gtcggtgacc
      481 attgtcgaga acgagggcaa tgcgcgtctg gtgaccgggc tggcgccagt ccacgtagtc
      541 gtggcgggga tcgagcgcat actccctcgg ctcgcggatc ttgaggtctt cctccagctc
      601 ctggcgggaa gcaatgccgg gcagaagctg acggcctaca cgtctgtgat caccgggcat
      661 cggcgtgagc acgaggtcgg tggcccagag cacgtgcacg tggtgctggt ggacaacggc
      721 cggagcagga tgctggccga cccaatcatg cgtcccgcct tgatgtgcat tcgctgcgcc
      781 gcgtgcctga atgtgtgtcc ggtctaccgg cgggttgggg gacacgcgta cggctggtgc
      841 tacccgggtc ccatcggcca ggtgcttggc gccgacatgt ggtccgagca ggaggggtac
      901 gacctggcct tcgggtcgac gctttgtggc gcgtgcgccg aggcctgccc ggtgcgtatc
      961 gacctgccca cggtgctgct gcgcctccgc cagcgggccg ctgaagcgga ggcgcccggc
     1021 ggagcggcgg agcggcgccg gttccgtctg tgggcgtttg cgatgaggca ccccaggatc
     1081 taccgcatgc tccctaactg gctgctccgg ctcgggatgc gggtggctcc ggagatacct
     1141 cgacgcgtcg ctccggggtg ggtggcaacg cgcgacctgc ccgagcccgc gccgcagacc
     1201 ttcgctcagc gctggcggag cacggggggc ttctcgcatg actgaagcgg ccgctgccgg
     1261 gctcaagagg atccgcgagg ccctgcagtc ctcc
//
LOCUS       DAOSPL010001501         1288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_105998_length_1288_cov_3.798054, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001501 DAOSPL010000000
VERSION     DAOSPL010001501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105998_length_1288_cov_3.798054"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..>1288
                     /locus_tag="PLQ54_20945"
     CDS             59..>1288
                     /locus_tag="PLQ54_20945"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOS95788.1"
                     /translation="MTSGQILNLSIVALYILLIVGVGTYFAFRKKDADRFMVAHKAMP
                     GWAVGLSMFGSYISSISFLANPGQTFKTNWMFAGFTLVTPVGLAIGSTAFLRFYRRSG
                     HVSAYTHLEERFGPWARTYAVLTYLILQMARMGTILYLLAQAVLPMLGGEAGNLSLQR
                     TLIIAIGLLITLYTLFGGIEAVVWTGVLQSIILIAGPIICVTTLLLGTPGGLGGVIRV
                     AGQAGKFSFGPYAWDWSIPTLWLVLLNSLIEHLRNWGIDQSYIQRYIAARSDREAVRS
                     IWIAGLLYMPVAAVFWFIGTALWAYYEAWPGRLPLGMAPDSVFPHFITHAIVPGLSGL
                     VIASIFAASMDSNLNSMATLTLIDGYKRYVNAEPDDRQALRVLWASTLFWGVASMGWA
                     LFMTLKGATSTIQFSANL"
BASE COUNT          211 a          435 c          392 g          250 t
ORIGIN      
        1 tgccccgctc ccataccccg gcaggtccga gcctgcggcg ggcgaagacc acggaccaat
       61 gacctccggc cagatcctca acctcagcat cgtggcgctg tatatcctgc tcattgtagg
      121 agtgggaacc tacttcgcgt tcagaaagaa ggacgctgac cgcttcatgg tcgcccacaa
      181 ggccatgccg ggttgggccg tgggcctttc catgttcggg agctacatca gcagcatcag
      241 cttcctcgcg aacccgggcc agacgttcaa aaccaactgg atgttcgcgg gcttcaccct
      301 ggtcacacca gtgggtctgg ccatcggctc gaccgcattc ctccggttct accggcggtc
      361 cgggcacgtg tcggcctaca cccacctcga ggaacgcttc ggcccctggg cgcgcacgta
      421 cgcggtactc acgtacctca tcttgcagat ggcccgcatg ggcaccatcc tctatctgct
      481 cgcccaggcc gtactgccga tgttgggcgg cgaggccggc aacctgagtc tgcagcggac
      541 gctgatcatc gctatcgggc tgctgatcac gctctacacg ctctttggtg gcatcgaagc
      601 ggtggtgtgg acgggcgtgc tccagagcat catcctgatc gccggcccca tcatctgcgt
      661 caccacgctg ctgctcggca cgcctggcgg tctgggaggg gtgatccggg tcgccggaca
      721 ggccggcaag ttcagcttcg gcccctatgc ttgggactgg agcatcccca cattgtggct
      781 ggtgctgctc aactcgctga tcgagcacct gcgcaactgg ggtatcgacc agagctacat
      841 ccagcggtac atcgcggcgc ggtcggaccg ggaggccgta cggagcatct ggattgccgg
      901 gctgctctac atgccggtgg ccgccgtgtt ctggttcatc ggcaccgcac tatgggcgta
      961 ttacgaggcc tggccggggc gcctgcccct gggtatggcg ccggactcgg tattcccgca
     1021 cttcattacc cacgccatcg tccccggcct gagcggcctc gtgatcgcgt ccatcttcgc
     1081 cgcctccatg gactcgaacc tcaacagcat ggccacgctg acactcatcg atggctacaa
     1141 acgctacgtg aacgcggaac ccgatgaccg gcaggcactg cgggtgctgt gggcatcgac
     1201 tctcttctgg ggcgtggcca gcatgggctg ggcactgttc atgaccctga agggggcgac
     1261 gtccacgatc cagttctcgg ccaacctc
//
LOCUS       DAOSPL010001502         1288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_106014_length_1288_cov_2.939173, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001502 DAOSPL010000000
VERSION     DAOSPL010001502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106014_length_1288_cov_2.939173"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PLQ54_20950"
     CDS             <1..643
                     /locus_tag="PLQ54_20950"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95789.1"
                     /translation="DDEGRFIQADTFARALVREALAQKPGRIVIDVTCTESLTADIEM
                     HGGEAVPAKCGYPNLLVKLSEGPQYLLGIEVSGHLFFNEPFFDFDDGTFAACRIASYL
                     ANTRQSMSSIVDDPMLPVRVQTPQIRLPAPDATKFQVVEELGRWLASHESVKRTDFTD
                     GVKAWISNGWALARAANTAPQIVLRVESNSAEDAEGIVGLVKPKLEELIQADS"
     gene            685..>1288
                     /locus_tag="PLQ54_20955"
     CDS             685..>1288
                     /locus_tag="PLQ54_20955"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS95790.1"
                     /translation="MKHRWTRREALQAASAAVALPYIITSRALGQDGQPGANDRVDIG
                     IVGLGGRARAMAATCALVPEVRIVAVCDCFKPACDSFINDMAQGQTWSAYDDIRRMCE
                     AERLDGVMIETTTHARAWATILAMQAGVDVYIEKPMCLTVAEGRYMVNAARRYGRVTQ
                     VGTQQRSMPINNWASDLVKSGALGRVRSVLAPNFLGPERWK"
BASE COUNT          235 a          417 c          421 g          215 t
ORIGIN      
        1 cgacgatgag gggcgattca tccaggccga caccttcgcc cgagccctcg tgcgcgaggc
       61 gctcgcccaa aagcccggcc ggatcgtcat cgacgtcacc tgcaccgaga gcttgaccgc
      121 cgacatcgag atgcacggcg gcgaggccgt cccggcgaag tgtggctatc cgaatctcct
      181 ggtcaaactg tcggaggggc cccagtatct gctcggcatc gaggtctcgg gccacctgtt
      241 cttcaacgag ccgttcttcg atttcgatga cggcacattt gccgcgtgcc ggatcgccag
      301 ctacctggcc aacacgcgcc agtccatgtc atccatcgtc gatgacccca tgctgccggt
      361 ccgcgttcag actccgcaga tccgcctgcc ggcccccgac gcaacgaagt tccaggtggt
      421 ggaggagctg ggccgctggc tcgcgtctca tgagtccgtg aaacgcacgg acttcaccga
      481 tggcgtcaaa gcgtggatca gcaacggctg ggccctcgca cgtgcggcca acaccgcgcc
      541 acagatcgtg ttgcgcgtcg agagcaactc ggccgaagac gccgagggga tcgtgggctt
      601 ggtgaagccc aagctcgagg agctcatcca ggcagactcg taagcagctg ggccgtcgcg
      661 agatcggtga ggagtgtgga ccacatgaag catcgctgga ctcgacgcga agcgctccag
      721 gcagccagcg ccgccgttgc gcttccctac atcatcacgt ctcgtgcact gggtcaggat
      781 ggccaaccgg gagccaacga tcgggtcgat atcgggattg ttggccttgg aggccgcgct
      841 cgagccatgg cagcgacgtg tgccctggtg cccgaggtgc gcatcgttgc cgtgtgtgac
      901 tgcttcaagc ccgcgtgcga cagtttcatc aacgatatgg cgcaaggcca gacctggagt
      961 gcctatgacg acatccgccg catgtgcgag gcggaaaggc tcgacggcgt gatgatcgag
     1021 acgaccacgc acgctcgggc atgggccacc atcctcgcca tgcaggcggg ggtggatgtc
     1081 tacatcgaga agcccatgtg cctcaccgtg gccgaaggtc gctacatggt caacgccgcc
     1141 cggcggtacg gccgggtgac ccaggtcggc acccagcagc gttcgatgcc catcaacaac
     1201 tgggcgagtg acctggtgaa gagcggcgcc ctcggacggg tgcgttcggt gctcgcaccc
     1261 aacttcctgg gccccgagcg gtggaagg
//
LOCUS       DAOSPL010001503         1286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_106233_length_1286_cov_3.254265, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001503 DAOSPL010000000
VERSION     DAOSPL010001503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106233_length_1286_cov_3.254265"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PLQ54_20960"
     CDS             <1..876
                     /locus_tag="PLQ54_20960"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS95791.1"
                     /translation="PLETQVVEQTEHWRIVEDELGGRTKAWSDREIGMSQWLVYPIRG
                     REQWEIFKERLNPASPARYPQYWDDLRRCWAGRDYPLGISGGSYYGWLRNWVGMEHLA
                     ILYYDDPELVREMTHTIADFVIETITRAADELDLDFALYWEDMAMKSGSLISPRLFRE
                     FMTPCYRRVTSFLAQRGIDIHLVDSDGNVDELIPLFLESGVNGIYPLEIAAGCDPVGY
                     RRQYGRDLILVGGIDKRALAKNRQAVYDEVMARIPPLLADGGYVPFVDHAVPPDVPFD
                     NFSYYMELVHELIGG"
     gene            901..>1286
                     /locus_tag="PLQ54_20965"
     CDS             901..>1286
                     /locus_tag="PLQ54_20965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95792.1"
                     /translation="MQSALALTCWGMIAVAADVPPVPQDVEPYPVVADIDFAEGPIFD
                     AYGNLYFVDYIRNGSIGVKTPEGEVRVLCQTGGRANGLKVDAEGFLIAADYEGKRILR
                     VDPAGADIRVLTQRFEDRPYIGPNDV"
BASE COUNT          236 a          394 c          416 g          240 t
ORIGIN      
        1 cctctcgaga ctcaggtggt tgagcagacc gagcactggc gcatcgtcga agacgaactg
       61 ggtgggcgga ccaaggcgtg gtccgatcgg gagatcggaa tgagccagtg gctcgtgtac
      121 ccgatccgcg ggcgggaaca gtgggagatc ttcaaagagc gcctcaatcc cgcatcgccc
      181 gcccgctacc cgcaatactg ggatgacttg cgccggtgct gggccggccg tgactatcct
      241 ctgggcatca gtggcggctc ctactacgga tggctccgca actgggtggg catggagcat
      301 ctggccatcc tctactacga cgaccccgag ctggtgcggg agatgaccca caccatcgcc
      361 gactttgtga tcgagacgat cactcgcgct gccgatgagc tcgacctcga cttcgcgctg
      421 tattgggaag acatggccat gaagtcaggc agtctgatct caccgaggct gttccgcgag
      481 ttcatgacgc cctgctatcg tcgcgtgacg agcttcctcg cccagcgcgg catcgacatc
      541 cacctggtcg actccgacgg caatgtggat gagctgatcc ccctgttcct ggagagcggc
      601 gtcaacggca tctacccact ggagatcgcg gcgggctgcg accccgtggg ttaccgtcgg
      661 cagtacggaa gggacctcat cctcgtcggt ggcatcgaca aacgggcgct ggccaagaac
      721 aggcaggcgg tgtatgacga ggtgatggcg cggattccgc ccctcttggc cgacgggggc
      781 tatgtgccct tcgtcgacca tgcggtgccc ccggatgtgc cattcgataa cttctcctac
      841 tacatggagc tcgtgcacga gcttatcggc ggctgatggg gccgccgcgc gggagggacc
      901 atgcagagcg cactcgcact cacctgctgg gggatgatcg cggtggccgc ggatgtacct
      961 ccggtgccac aggacgtcga gccgtatccg gttgtggccg acatcgattt cgctgaaggg
     1021 cccatctttg acgcctacgg gaatctttac tttgtcgact acatccggaa tgggagcatc
     1081 ggcgtcaaga cacccgaagg cgaggttcgg gtgctctgcc agacgggagg ccgagccaac
     1141 ggtctcaagg tggacgccga aggcttcctg atcgcggccg actacgaggg caaacgcatc
     1201 cttcgggtcg atccagccgg ggccgatatc cgtgtgctga ctcagcgatt cgaagaccgg
     1261 ccatacattg gccccaatga cgtctg
//
LOCUS       DAOSPL010001504         1285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_106360_length_1285_cov_3.789431, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001504 DAOSPL010000000
VERSION     DAOSPL010001504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106360_length_1285_cov_3.789431"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="PLQ54_20970"
     CDS             <1..976
                     /locus_tag="PLQ54_20970"
                     /inference="COORDINATES: protein motif:HMM:NF021645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HOS95793.1"
                     /translation="AGMRFAILYGIPNQPLYDGLEEDALIAYTWQRYLETGDETWPLQ
                     FPMVKSALRAMDAISATAKEQWNQDLRGFMVTGASKRGWTTYLTGIADPERVVAMAPM
                     VFDILNLPVSAPHQKDFWGDYSPQIEDYTKTGLVGALETPRGFRLSYMVDPYSYRDRL
                     HMPKLVILGSNDPYWPTDAVNYYWNGLPMPRSLLILPNSGHDLGDRERLLASITTFSR
                     IIASGAQVPAMTWDYTEKPEGSTLTMSTAGTVKEARVWVAHAPVCDFRPAKWEEAPAA
                     EQAGSYVATIDRARGQWTACYGELELEVAGRTLYLSTQPRVMHPLAAE"
     gene            complement(1045..>1285)
                     /locus_tag="PLQ54_20975"
     CDS             complement(1045..>1285)
                     /locus_tag="PLQ54_20975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95794.1"
                     /translation="HQAMAHTGYFAVCGAKGCIRACMDSLEKRACIEQHGFATPVWPR
                     PDWVLSPPETDETGGIAEGKFADFRDPDPNPGGWK"
BASE COUNT          209 a          435 c          435 g          206 t
ORIGIN      
        1 ggcgggcatg cggttcgcca tcctctatgg aatcccgaac cagccgctct acgacggcct
       61 cgaggaagat gccctcatcg cctatacgtg gcagaggtac ctggagacgg gcgatgagac
      121 ctggcccctg cagttcccga tggtgaagtc ggccctgcgc gccatggacg ccatcagtgc
      181 cacggcgaag gagcagtgga accaggacct gcgggggttc atggtcaccg gggcgtcgaa
      241 gcggggctgg accacctacc tgactgggat tgctgatcct gagcgcgtgg tggccatggc
      301 gcccatggtg ttcgacatcc tcaacctgcc ggtctcggcg ccgcaccaga aggacttctg
      361 gggcgactac agcccgcaga tcgaggacta cacgaagacc ggcctcgtgg gcgccctcga
      421 gacaccacgc ggctttcggc tctcgtacat ggtggatccg tactcctacc gcgaccggct
      481 ccacatgccg aagctggtca ttctgggaag caacgatccc tactggccca ccgatgcggt
      541 gaactactac tggaacggac ttcccatgcc ccgcagcctg ctgatcctgc ccaactccgg
      601 gcacgacctg ggcgaccgcg agcgcctgct ggcctcgatc acgacgttct cgcgcatcat
      661 cgcctcgggc gcgcaggtgc cagcgatgac ctgggattac acggagaagc cggagggttc
      721 gacgctcacg atgagcaccg ccggcacggt gaaggaggcc cgcgtgtggg tggctcacgc
      781 cccggtgtgt gacttccggc cggccaaatg ggaggaggcg cccgcggcgg agcaggccgg
      841 gagctacgtc gccaccatcg accgtgcgcg cgggcaatgg accgcctgct acggggagct
      901 cgagcttgag gtcgcggggc ggacgctgta cctctcgacc cagccgcggg tgatgcaccc
      961 cctggcagcg gagtaggggc ttgcgcaccg cgggacattg gcgactggcc gctgagcgct
     1021 ccgcgccagg caccgtccct tttcctactt ccacccgccc gggttcggat ccgggtcgcg
     1081 gaaatcggcg aacttgccct cggcgatgcc gccggtctcg tcggtctccg ggggcgagag
     1141 cacccagtcc ggtcgcggcc agacgggcgt ggcgaagccg tgctgctcga tgcacgcacg
     1201 cttctccagg ctgtccatgc acgctcggat gcatcccttg gccccgcaga cggcgaaata
     1261 gccggtgtgc gccatggcct ggtgg
//
LOCUS       DAOSPL010001505         1285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_106380_length_1285_cov_2.813008, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001505 DAOSPL010000000
VERSION     DAOSPL010001505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106380_length_1285_cov_2.813008"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="PLQ54_20980"
     CDS             <1..921
                     /locus_tag="PLQ54_20980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95795.1"
                     /translation="CDEQWDLSPLKATNNVNGVGDMAREEVYTLRDSGLQRVQEAMVR
                     RIVHELAGFDNVYYEICNEPYFGGVTLPWQHRIADVIWEAETARPHRHLIAQNIANGS
                     ALVDTPHPAISILNFHYAAPPDAVTRNYHLGRVIADDETGFRGTADRPYRAEAWHFLM
                     AGGAVFDHLDYSFTAQHEDGTFPLPEGQPGGGGRAFRHQMGVLKRFIESFDFVRMAPS
                     TDTVRSSTGGVSCRALVEPGRAFAVYVEGGTQVGIRLEAPTGRYVAEWVNPRTGETIR
                     GPELDHPGGELALSSPAYEEDIALRLVRRD"
     gene            complement(1063..>1285)
                     /locus_tag="PLQ54_20985"
     CDS             complement(1063..>1285)
                     /locus_tag="PLQ54_20985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95796.1"
                     /translation="FRWNRFIHGELELIGSNASAGAWPEAVRLAVDEGLPLGRLISAR
                     VAAARFADAIGMAEADRACVKVILDWTAA"
BASE COUNT          209 a          437 c          438 g          201 t
ORIGIN      
        1 tgtgacgagc agtgggatct gagtcccctg aaggcgacga acaacgtcaa cggtgtcggg
       61 gacatggcac gtgaggaggt ctacaccctg cgcgactcgg gtctccagcg cgttcaggaa
      121 gccatggtgc gccgcatcgt ccatgagctg gccggtttcg acaacgtcta ctatgagatc
      181 tgcaacgagc cctatttcgg aggcgtgacc cttccgtggc agcaccgcat cgccgatgtg
      241 atctgggagg ccgaaaccgc gcgccctcat cggcacctga tcgcccagaa catcgcgaac
      301 ggatctgccc tcgtcgacac accgcacccc gccatctcca tcctgaactt ccactacgcg
      361 gcgccgccgg acgctgtgac gcggaactat catctgggac gcgtcatcgc tgacgacgag
      421 accggtttcc ggggcaccgc cgatcggcca taccgcgccg aggcttggca cttccttatg
      481 gccggtgggg cggtgttcga ccacctggac tactccttca ccgcccagca tgaggacggg
      541 accttcccgc tgcccgaggg gcagccgggc gggggcgggc gggcattccg tcaccagatg
      601 ggggtcctca agcgcttcat cgagagcttc gacttcgtgc ggatggcccc gtcgactgac
      661 acggtccgct cgagcaccgg gggcgtctcg tgccgcgccc tcgttgagcc aggccgggcc
      721 ttcgcggtgt acgtcgaggg cggcacccag gtgggcattc gcctggaggc ccccacaggg
      781 cgctatgtgg ccgagtgggt caatcccaga accggggaga ctatccgggg accggagctg
      841 gaccacccgg gtggcgagtt ggcgctatcc tctcccgcgt acgaggagga catcgcgttg
      901 cgcttggttc ggcgcgactg acggtggcgc ccagaggcgc agcggctgat gaacccgccg
      961 ggcccggggc ggctggtgaa cccgccgacc agtccctgcc gcgcgaatgc ttggcccgtg
     1021 agcgccggta cggccagaca cctccacgca tgggggcgag cctcaggctg ccgtccagtc
     1081 gagaatgacc ttgacgcacg cgcggtcggc ctcggccatg ccgatggcgt ctgcgaaacg
     1141 cgccgccgcg acgcgggcgg agatcaggcg gcccagtggc aacccctcat cgacggcgag
     1201 ccggaccgcc tcgggccagg cgccagcact ggcgttgctg ccgatcagct cgagttcgcc
     1261 gtggatgaac cgattccagc ggaag
//
LOCUS       DAOSPL010001506         1285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_106401_length_1285_cov_2.372358, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001506 DAOSPL010000000
VERSION     DAOSPL010001506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106401_length_1285_cov_2.372358"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1285
                     /locus_tag="PLQ54_20990"
     CDS             <1..>1285
                     /locus_tag="PLQ54_20990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95797.1"
                     /translation="RGALLAAMDDPDRGDVRAAWIAHNADTIDSLMAHVSAAVRAVNP
                     GIAMGLMTAGPGDLYCGAELPRWLRSLGATKLRPGGGFYADTTPGAMIEKAFDVGRGS
                     RVVRDMREIADIQYELENFPYQKLGKSATALISECTLALAMGCNGVALNMLPMWGGPF
                     DDIGAFLDRLPAARAVWERLVDDAAGLPTAGLWPAWHAKLQARRPVRVGESWLGSMGE
                     HGTSVPYVLSGIGVPLSVEPWDGVGPPVYGTVLGGRAAEGFTDDELRVLLSGGVLMDT
                     ATLEILSARGLGELTGVRVARRIDNGAMEQLTEDALNGGYAGQIRDARIEFWGNAQGK
                     ADILEARADGVRVLANTHDYFRRCHGPCLSVYENELGGRVAVMGYAPWMFLHSVSKRA
                     QLLNIADWITGEGLPVRVEEPVALVPFVRMAEDRS"
BASE COUNT          196 a          353 c          514 g          222 t
ORIGIN      
        1 atcgcggggc gctgctggcg gccatggacg acccggatcg gggtgacgtc cgtgcggcgt
       61 ggatcgctca caacgccgac acgatcgact cgctgatggc tcacgtgtcg gcggcggtgc
      121 gtgcggtgaa ccctggtatc gccatggggc tgatgacggc tgggccgggc gacctgtatt
      181 gcggagcgga gctgccgcgg tggctgaggt cgctcggggc gacgaagcta cgaccgggtg
      241 gcgggttcta cgcggacaca acgccgggag ccatgatcga gaaggcgttt gacgttggtc
      301 gaggctcgcg ggtcgtgcgg gacatgcgag agattgccga cattcagtat gagctggaga
      361 actttccata ccagaagctc gggaagtcgg ccacggcgct catcagcgag tgcacactcg
      421 ccctggccat gggctgcaat ggcgtggcgc tgaacatgct gcccatgtgg ggcgggccgt
      481 ttgacgatat cggtgcgttc ctcgatcggc tgccggcggc ccgggcggtg tgggagcggt
      541 tggtggacga cgcggcgggc ctccccacgg ctggcctgtg gccggcatgg cacgcgaagc
      601 ttcaggcgag aaggcctgtt cgcgtgggcg agagctggct ggggagcatg ggggagcatg
      661 gcacctcagt cccgtatgtg ctgtcgggga tcggcgtgcc actctcggtc gagccatggg
      721 acggagtggg gccgccggtc tacggtacgg tgctgggcgg ccgggccgcc gaggggttca
      781 cggacgacga gctgcgggta ctgctgtcgg gcggcgtgct gatggacacg gcgacgctgg
      841 agatcctctc ggctcggggg cttggtgagc tcacgggtgt tcgcgtggct cgaaggatcg
      901 acaacggggc aatggagcag ttgacggagg atgctctcaa cggcgggtat gcggggcaga
      961 ttcgcgacgc gcggatcgag ttctggggca acgcgcaggg caaggcagat atcctcgagg
     1021 cccgagcgga cggggtgcgt gtgctggcga acacccacga ctacttccgg cggtgccatg
     1081 gtccgtgctt gagcgtatat gagaacgagc tgggtggtcg cgtggccgtc atgggttacg
     1141 cgccgtggat gttcctgcac tcggtgtcga agcgggcaca actcctgaac atcgccgact
     1201 ggatcacggg agagggcctg ccggttcggg tcgaggaacc cgtggcgctg gtcccgttcg
     1261 tgcggatggc cgaagaccgg tccag
//
LOCUS       DAOSPL010001507         1280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_107018_length_1280_cov_2.397551, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001507 DAOSPL010000000
VERSION     DAOSPL010001507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107018_length_1280_cov_2.397551"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1280)
                     /locus_tag="PLQ54_20995"
     CDS             complement(<1..>1280)
                     /locus_tag="PLQ54_20995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95798.1"
                     /translation="AVLCREWNWTFGSAHTVTRRLKVFNNTHLAGDIAVGWQLHVGGA
                     KVAGEEKMVSLPAGGAQELSVQFTVPATTSRTEGEFILTCARDGNEVFREVKPVAVLG
                     PSTAPKPGLPREAVAVLDPQGAVRARLGAMGIAFTEVDSPAAIGPEATLIIVGRDALS
                     PREATDPVWVALASRGARVLVLDQQNPLRYLAVPADCTPTDFVGRVAFAENLDHPAFA
                     GLGQQDFFTWSGDHVVYRNAYRKATRGAVSLVQCAEQLGCSALLECPVEDGLLVLCQL
                     VVGEKLDGDPVAQRLFDNLVSYCAAYAPVRRSTAVVMSPDTPAYRMLAGSGLQFDEAP
                     SVLAAMADGKHDVIVFDATPQSLDELAGSAAAVRSFAEKGGWLMAWGLTPEGLGSFNR
                     LVGVEHMIRPFELERVTLPAVRDPLLLGRREASP"
BASE COUNT          216 a          464 c          423 g          177 t
ORIGIN      
        1 cgggtgacgc ttcccgccgt ccgagaagca gtggatcgcg gacggcggga agcgtcaccc
       61 gctcgagttc gaacggccgg atcatgtgct cgacgcccac gagtcggttg aaactcccga
      121 gtccctcggg cgtgagcccc caggccatca gccatccgcc cttctcggcg aagctccgga
      181 cggccgcggc actgccggcg agctcatcca atgactgggg cgtagcatcg aacacgatga
      241 cgtcatgctt accgtctgcc atggcggcca gcacactggg agcctcgtcg aactgaaggc
      301 ccgagccggc cagcatccgg taggccggcg tgtcaggcga catgacgacc gccgtgctcc
      361 gccgcaccgg cgcgtaggcg gcgcagtagc tcaccaggtt gtcgaacagc cgctgagcca
      421 cggggtcgcc atcgagcttc tcgcctacca ccagctggca cagcaccagc aagccgtcct
      481 cgaccgggca ctccaggagc gccgagcagc cgagctgctc ggcgcactgc accagcgata
      541 cggcgccgcg cgtcgccttg cggtaggcat tgcggtagac gacatggtcg ccggaccacg
      601 tgaagaagtc ctgctgcccc aggcccgcga acgccggatg gtcgagattc tctgcgaacg
      661 ccacacggcc gacgaagtcc gtcggggtgc agtccgccgg gacggccagg tatcgaaggg
      721 ggttctgctg atccagcacc agcacgcgcg ccccccgaga cgccagcgcc acccaaacgg
      781 gatccgtcgc ctcgcgggga gagagagcat ctctgccgac gatgatgagc gtcgcttcgg
      841 gcccgatcgc cgcgggcgag tcgacctcgg tgaacgcgat gcccatcgcg ccgagccgcg
      901 ctctgacggc cccctgcggg tcgagcacag caacggcctc gcgtggcaga cccggcttcg
      961 gggccgtcga ggggcccagc acggcgacgg gcttcacctc gcggaagacc tcattgccgt
     1021 cgcgcgcgca ggtcaagatg aactcgccct cggtccggct ggtcgtggcg ggcacggtga
     1081 actgcaccga gagctcttgc gccccgccag cgggaagcga gaccatcttc tcctcaccgg
     1141 ccaccttcgc cccgccgaca tggagctgcc agccgaccgc gatgtcgccg gccaggtgcg
     1201 tgttgttgaa caccttgagc cgccgcgtca ccgtgtgggc agacccgaag gtccagttcc
     1261 actcccggca cagtacggcc
//
LOCUS       DAOSPL010001508         1276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_107503_length_1276_cov_2.510238, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001508 DAOSPL010000000
VERSION     DAOSPL010001508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107503_length_1276_cov_2.510238"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLQ54_21000"
     CDS             <1..228
                     /locus_tag="PLQ54_21000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HOS95799.1"
                     /translation="EEVRARGADVIAVATDGNQAIGELSRDVLWVPRTDPHLAPILTV
                     IPLQLLAYHIAGIKGLNVDQPRNLAKTVTVE"
     gene            complement(331..993)
                     /locus_tag="PLQ54_21005"
     CDS             complement(331..993)
                     /locus_tag="PLQ54_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HOS95800.1"
                     /translation="MAEPGPAEDLLNRIRGRYSTILADPPWQFQNRTGKVAPEHKRLM
                     RYPTMELEEIRTLPVAKLGAARSHLYLWVPNALLREGLSVMEAWGYAYKSNLVWYKIR
                     KDGGPDGRGVGFYFRNVTELVLFGVRGSMRTLPPGRSQVNLVSSRKREHSRKPDEIYD
                     LVESCSPGPYLELFARFSRQGWTQWGNEDVEENTHHDVAMRQAHSPAQMRLLKSPSGY
                     GG"
     gene            complement(1034..>1276)
                     /locus_tag="PLQ54_21010"
     CDS             complement(1034..>1276)
                     /locus_tag="PLQ54_21010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOS95801.1"
                     /translation="IREIDALYPYYHPTARQISMISRAAESRGCFEDGWAHAFVADKF
                     GEATTHWRKLEDRVRRGVGNPCPLLFLGIPKEVVVF"
BASE COUNT          212 a          411 c          413 g          240 t
ORIGIN      
        1 gaggaggtcc gcgcccgcgg cgccgacgtg atcgccgtgg ccaccgacgg caaccaggcg
       61 atcggcgagc tctccaggga cgtgctgtgg gtgccgagga ccgacccgca cctcgctccg
      121 atcctcaccg tcatcccgct ccagcttctg gcgtaccaca tagccggcat caagggcctg
      181 aacgtcgacc agcccaggaa cctggccaag accgtcacgg tggaatgatg ctcccgaatc
      241 ggttgctggg cgcccggcgg caggtcgacg cgcttggctc tctcgccgcc gcgcatacac
      301 ggtcgtgcca gtatccggtg gcagggtgca tcagccgccg tatccgcttg ggctcttgag
      361 gagccgcatc tgcgcgggcg aatgagcctg tcgcatcgcc acgtcgtggt gggtgttctc
      421 ctcgacatcc tcgttgcccc actgtgtcca gccttggcgc gagaagcgcg cgaacagctc
      481 gaggtagggc cctggtgagc acgattcgac gaggtcgtag atctcatcgg gcttgcgtga
      541 gtgctcccgt tttcggctcg ataccagatt cacttggctg cgtcccggag gaagcgtgcg
      601 catgctgcca cgcacaccga acagcaccaa ctcagtgacg ttgcggaagt agaaccccac
      661 acctcggccg tccggacctc cgtccttgcg gatcttgtac cagacgaggt tcgacttgta
      721 cgcgtagccc cacgcttcca tcacgctgag gccctctctg aggagggcgt ttgggaccca
      781 gagatacaga tgagagcgtg cggccccgag tttcgccacc ggcaacgtcc gtatctcctc
      841 gagctccatg gttgggtagc gcatgaggcg cttgtgctcg ggtgccacct tgccggtacg
      901 attctgaaac tgccaggggg gatcggcgag gatggtcgag tagcgcccgc ggattctgtt
      961 gagcagatct tcggcggggc caggctctgc gatgctgtgg atgcgcgcgt ctgtcacggt
     1021 gtctacacgg gtcctagaag accacgacct ccttcgggat gcccaggaag agcaaagggc
     1081 acgggttgcc tactcccctc cgcacgcgat cctcaagctt gcgccagtga gtggtggcct
     1141 cgccgaactt gtcggcgacg aaggcgtggg cccatccatc ctcgaagcag ccccttgatt
     1201 ccgccgcacg actgatcatg ctgatctgcc gcgccgtggg gtggtagtaa gggtagaggg
     1261 cgtcgatctc gcggat
//
LOCUS       DAOSPL010001509         1275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_107597_length_1275_cov_3.345082, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001509 DAOSPL010000000
VERSION     DAOSPL010001509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107597_length_1275_cov_3.345082"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            537..>1275
                     /locus_tag="PLQ54_21015"
     CDS             537..>1275
                     /locus_tag="PLQ54_21015"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOS95802.1"
                     /translation="MPSIYDVAREARVSIATVSRVFRGTAPVSAATRERVLAAARALG
                     YRPNRIARALAEGRTRAIGVMLPTDISNPFYAQLAQHVASCARTASYDVAVGLPPDDS
                     VESFLETTAALEERQVDGLLLCASCSRVAAYAARRPQGACPAVAVGCLPTVDVPLVSA
                     DEEYGGYLITNHLLALGHRRVAFLCAPGQPGTAHPQGREHGYIRAMDARGLEPLLWHG
                     VGTLESGYTRVRDLMAEHGDLTALVAVN"
BASE COUNT          193 a          406 c          443 g          233 t
ORIGIN      
        1 ggtaaaggtg ggcttggact cggcgtgacg ggtccaggtc ggagcggtga ctctgggagt
       61 cgacgagtcc cccgagtcgt ccggcgggtg tgcgtcctga aagcattccg gccactgcgg
      121 caagggaaga cacgctacgg caacctgtcg cccggtaccg cgagcacccg tgtcgggggc
      181 accgcccccg cagcggagag caatggacgg aatgggggat cgttggggtg gggatgtcgg
      241 ctcttgtcaa cgcagaacag catggcctat tcgctcagcc tggggcagcc cggttcgagc
      301 gatccgtgtt gcggttcgta taaggtgagt atagtacctc caggcatttc cgccagacag
      361 cgcaagatcg gggagcgagt caggccatgt cggccgcggc cagcaacgcg atgttgatgg
      421 tgttgtccgg gtatgccatg gacccgcggg cttgcgccgg tgtggtccgg tgttcacaat
      481 gggtccggga tcgcttctgt ccctcgtgtt cacacaccag gctccggtga tacctcatgc
      541 ccagtatcta tgatgtcgcc cgcgaggccc gggtatccat cgcgacggtc tcgcgggtgt
      601 tccgtggcac ggctccggtg agtgccgcaa cgcgggagcg tgttctcgcc gccgcccgtg
      661 cgctggggta ccgccccaat cggatcgcac gggccctggc cgaggggaga actcgcgcga
      721 tcggcgtcat gctccccacc gatatctcca atccgttcta tgcgcaactg gcgcagcatg
      781 tggccagctg cgcccgcacc gcatcatacg acgttgcggt gggcctgccc cccgacgact
      841 cggtcgagag cttcctcgag accactgcgg ccctggagga acgccaggtg gatggcctgc
      901 tgttgtgtgc ctcctgctcg cgagtggcgg cctacgccgc ccggcgtcct cagggagcgt
      961 gtcccgcggt tgccgtcggc tgcctcccta ccgtcgacgt gcccctggtg tcggctgacg
     1021 aggaatatgg gggatatctt atcaccaatc acctcctggc cctggggcat cgacgagtcg
     1081 ccttcctgtg tgcgccgggc cagcccggaa ccgcgcatcc ccaggggcgc gagcatggct
     1141 acattcgcgc catggacgct cgcgggctcg agccgctcct atggcatgga gtcggcacgc
     1201 tcgagtcggg gtacacgagg gtacgggact tgatggcgga acacggcgac ctgacggcgc
     1261 tcgttgccgt gaacg
//
LOCUS       DAOSPL010001510         1275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_107655_length_1275_cov_2.228689, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001510 DAOSPL010000000
VERSION     DAOSPL010001510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107655_length_1275_cov_2.228689"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..>1275
                     /locus_tag="PLQ54_21020"
     CDS             107..>1275
                     /locus_tag="PLQ54_21020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95803.1"
                     /translation="MATDWFGDGLRLALLGYGLPPLLLAAAALWLSRQRTVPQVPWGW
                     LALFGAVHGTSQWFGEFPRGVAVVSWAEHARWCLVAASFGILYEFGRRGARGQGGWAP
                     PPWATIVLGTLASCGLAAGTAGLNAASRYTFGLAGGLLAASTLWRAAWARDARCCPAL
                     SLAAVAMLLFGLNSAAFAPRSALWPASWLNYEALQGSVGPAIHVSRGACVLICAACLW
                     TVARPWHRPAGVRWSLWWPLPVTIVAVTVAGRVAVNWHGEALDRQMRATLMAQVVGIA
                     DALDPEAVSRLTFTEADSQSVTFRQIRWQLRAFAQYARLRSVYTLAVRSGHMCFGPES
                     LDEDDPWASPPGTPYSPPTDDLPRVFESGTPTVAGPVTDEYGSFVSGLAPVLDPRT"
BASE COUNT          139 a          437 c          453 g          246 t
ORIGIN      
        1 gcgttgtggg gcagggtgat gcatgtgccg ccctgggatc cccgcgatgt cgccgcgtcg
       61 tctccggccc cgggtacccc ggcatgccgg gttcagggag tcgcgcatgg ccactgactg
      121 gtttggcgac ggactcagac tcgccctgtt gggctacggg ctgccacccc tgttgctggc
      181 cgcggcggcg ctctggctgt cacggcagag gaccgtgccc caagtgccgt ggggatggct
      241 cgccctcttt ggtgccgtcc atggcaccag tcagtggttt ggcgagttcc ctcgtggtgt
      301 tgccgtggtg tcgtgggccg agcacgcgcg ctggtgcctt gtggcggctt cctttggcat
      361 cctctacgag tttggccgtc gaggcgcccg cggccagggc ggctgggctc ctcctccttg
      421 ggccaccatc gtgctgggca cgctggcgtc atgcgggctg gccgcaggga cagcgggtct
      481 caacgcggca agccggtaca cctttgggct cgcgggcggg ctgttggctg cgtcgacact
      541 ctggcgcgcc gcgtgggccc gcgatgcacg ttgttgtccg gccctgtccc tggctgcggt
      601 ggccatgctg ctgttcggcc tgaactcggc ggcgttcgct ccgcgcagcg ctctctggcc
      661 ggcctcatgg ctgaactatg aggcgctgca gggctcggtg gggcccgcca tccacgtgtc
      721 gcggggggcg tgcgtcctca tctgcgccgc gtgcctctgg accgtcgcgc gcccgtggca
      781 ccgtcccgcg ggggtgcgtt ggtctctctg gtggcccctc ccggtgacca tcgtcgccgt
      841 gaccgtcgcc gggagggtgg cggtgaactg gcacggcgag gctctcgatc ggcagatgcg
      901 cgcgacactg atggcccagg tcgttgggat cgccgatgcg ctggaccccg aggccgtctc
      961 ccgattgacg ttcacggagg ccgattcaca gagcgtcaca ttccggcaga tccgctggca
     1021 gctcagagcc ttcgctcagt atgctcgctt gcgcagcgtg tacaccctag ccgtgcggtc
     1081 ggggcacatg tgctttggcc ccgagagcct ggacgaggac gatccgtggg cctcgccacc
     1141 tggcaccccc tactcaccac cgaccgacga cctgccgcgc gtgttcgagt caggcactcc
     1201 cacggtggcc ggtccggtga ccgacgagta cggctcgttc gtctcaggcc tcgcacccgt
     1261 gctagatccc cgaac
//
LOCUS       DAOSPL010001511         1273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_107886_length_1273_cov_2.659278, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001511 DAOSPL010000000
VERSION     DAOSPL010001511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107886_length_1273_cov_2.659278"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PLQ54_21025"
     CDS             <1..437
                     /locus_tag="PLQ54_21025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95804.1"
                     /translation="WDPADSTEWLEDRMARLAHAARPPYGSRSWARLWERTQLDLTAR
                     GDWFARAVLAPLNEARETDLAARARLAIEETTAYLAAFRARYGHYPTALARLAPELTD
                     ALPDDPYTDEPFRYEPAADGYRLGSPKLDEYEAGRGAELDRP"
     gene            711..>1273
                     /locus_tag="PLQ54_21030"
     CDS             711..>1273
                     /locus_tag="PLQ54_21030"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOS95805.1"
                     /translation="MPPKPLAPVVPLAFAVLGQIGPATAAGGPREAGALRCDQIHGVR
                     LDLAGPMGERVRANIDNWLLRAPEANPGMLEMFRRRDREPVPDLVPWAGEFVGKYLIS
                     AIQACRMTTDGRLKPYIASVLRDLIATQADDGYLGPFRRADRLLGQWDLWGHYHAMLA
                     LLMWHEDTGDAEALGCAMRAGDLICNT"
BASE COUNT          185 a          496 c          407 g          185 t
ORIGIN      
        1 catgggatcc tgcggactcg accgagtggc tcgaggatcg catggcccgc ctggctcatg
       61 ccgcccggcc gccctacggc agccgatcct gggctcgcct gtgggagcgg acccagctcg
      121 atctgaccgc ccgaggcgac tggttcgccc gtgccgttct cgcccccctc aatgaagccc
      181 gggagaccga cctcgccgcc cgtgcccgcc tggccatcga agagaccacc gcctacctcg
      241 ccgccttcag ggcccggtat gggcactatc cgacggcact ggcccggctg gctcccgagc
      301 tcaccgacgc actgcccgac gatccctaca ccgacgagcc attccgttac gagcccgccg
      361 ccgacgggta ccgcctcggc tcaccgaagc tcgacgagta cgaggccggc agaggtgcgg
      421 agcttgaccg tccataggcg cgaccgggcg gcgggagccc cggaggagcg ttgaccgcgc
      481 ctcctcagac gaagcctcca tcgtgttacc catgtgcccg gatcatgccc ccttcggccg
      541 cacccctgtt cggcctctcc agaagacggg tcagtgcggt gtacggcccg atcacgccaa
      601 cgctcgggag ccgcggggcg gcgccaggca gggctctgcc gagggcgggg aggccggcct
      661 cggcccttca ccgaatcccc acctccgcag gctcaccctg gaggtcccac atgccaccga
      721 aacctctcgc gcccgtggtc cccctcgcct tcgccgtgct cggccagatc ggtcccgcca
      781 cggctgccgg cggcccccgc gaggccggcg cgctgcgctg cgaccagatc cacggcgttc
      841 gcctcgatct cgccgggccc atgggcgagc gcgtgcgggc gaacatcgac aactggctgc
      901 tgcgagcgcc tgaagccaac ccgggcatgc tcgagatgtt ccgtcggcgc gaccgagagc
      961 ccgtgcccga cctggtgccg tgggccggcg agttcgtggg gaagtacctc atctcggcca
     1021 tccaggcgtg ccggatgacc accgacggcc gcctgaagcc ctacatcgcc tcggtcctcc
     1081 gggacctcat cgcgacccaa gccgacgatg gctacctcgg cccattccgc agggccgatc
     1141 gcctcttggg ccagtgggac ctctggggcc actaccacgc catgctggca ctgctgatgt
     1201 ggcatgagga caccggcgat gccgaggctc tgggttgcgc catgcgcgcc ggcgacctca
     1261 tctgcaacac ctt
//
LOCUS       DAOSPL010001512         1272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_108001_length_1272_cov_3.193098, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001512 DAOSPL010000000
VERSION     DAOSPL010001512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108001_length_1272_cov_3.193098"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1272
                     /locus_tag="PLQ54_21035"
     CDS             <1..>1272
                     /locus_tag="PLQ54_21035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95806.1"
                     /translation="LPTAVRVRQTFGFCADGRTLRVRTRLRALAAPVVIQRVGLLDLR
                     LPGQTLRRIGPEHVSCPVVGDRIFAGVEHPSAMTQVDGDALHIAQHSYITVGSQWVDV
                     PSAVFGAASLEDVTRGAEAVRVAFLRYLDTVWVKPKDMHIHYNNWWTMPVPFTEHDVL
                     ANIADLKASLYDRTGFFFDSYAMDMGWSDPHTVWQVNGEAYPEGFRRIARALAPMGCR
                     PGLWVSPSSCYPPALDNEWLDGEGYETFTNAAGTRYACLARGGRYQQSFKSAVLSHVR
                     DGQMAHVKFDGLAWPCPSAEHGHRPASESYQPIAEGLLDVFDALRAQAPDIALEPTCL
                     GYFPSPWWLMHTPFVIGPFGDDCPRGVCPAPDWMESLTTARDVANLRGRDAFWMPSSA
                     LECFDIIVQCSGSFYNHAVMAVARGHWFQST"
BASE COUNT          187 a          408 c          428 g          249 t
ORIGIN      
        1 cctgcccact gccgtgcggg tgcggcagac ctttggcttc tgcgcggacg gtcggactct
       61 gcgggtacgt acgcgtctgc gcgcccttgc ggccccggtg gtcatccagc gggtcgggct
      121 cctggatctg cgcctgcccg gacagacact gcggcgcata ggccccgagc atgtctcatg
      181 ccccgtggtg ggtgatcgga tcttcgccgg ggtcgaacac ccgtcggcca tgactcaggt
      241 cgatggcgat gccctccaca ttgcccagca ctcgtacatc accgtcggca gccagtgggt
      301 cgacgtgcct tcggctgtgt tcggggcggc ctcgctggag gacgtcacgc gcggcgccga
      361 agcggtgcgc gtggccttcc tgcggtatct tgacacggtg tgggtgaagc ccaaggacat
      421 gcacatccac tacaacaact ggtggacgat gccggtccca ttcacggaac acgacgtgct
      481 cgccaacatt gcggacctga aggcgagcct gtacgaccgt acgggcttct tctttgactc
      541 gtatgccatg gacatgggtt ggtcggaccc gcacacggtg tggcaggtga acggagaggc
      601 ctaccccgag ggctttcgcc ggatcgcgag ggctctggcg cccatgggct gccggccggg
      661 gctgtgggtc tcgccaagta gctgctatcc cccggcgctg gacaacgagt ggctggatgg
      721 ggagggttac gagacgttca ccaacgcggc cggcactcgc tatgcgtgcc tggctcgtgg
      781 cggacggtat cagcagtcgt tcaagtcggc ggtcctgagc catgtgcggg acggccagat
      841 ggcccatgtg aagttcgacg gcctggcatg gccctgcccc tctgccgagc acggccaccg
      901 gccggcctct gagtcctacc agcccatcgc cgagggcttg ttggacgtgt tcgacgccct
      961 gcgtgcccag gccccggaca tcgcactcga acccacatgt ctcgggtact tccctagccc
     1021 gtggtggctg atgcacaccc cgtttgtgat cggcccgttc ggcgatgatt gcccgcgagg
     1081 agtgtgtccg gccccggact ggatggagtc gctcaccacg gcgcgcgatg tggcgaatct
     1141 gcgtggtcgc gatgcgttct ggatgcccag ctcagcactg gagtgcttcg atatcatcgt
     1201 gcagtgttcg ggatcgttct acaaccacgc tgtcatggcg gtcgctcgtg gccactggtt
     1261 tcagtcgacg ta
//
LOCUS       DAOSPL010001513         1272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_108019_length_1272_cov_2.843878, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001513 DAOSPL010000000
VERSION     DAOSPL010001513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108019_length_1272_cov_2.843878"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..>1272
                     /locus_tag="PLQ54_21040"
     CDS             48..>1272
                     /locus_tag="PLQ54_21040"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOS95807.1"
                     /translation="MDEAGLDPANAQHRADFGFEVLRRFQDRLFATVRSHHPEATVFF
                     NSGHVGPKHRRIIDAFTHLELESLPSGGWGYMHFPLAQRFARGLDRDTMGMTGKFHTS
                     WGDFQSFKNPAALDFECLNMLALGAKCSIGDQLHPRGRICKDTYALIGRTYAKVEAAE
                     PWCVGAKPLSDIAVLTPEEFASGHSHAGLPNEALGAARILQESRHQFDLADSHTDLAA
                     YRVVILPDSIPVDSGLAAKLSAFVAHGGAILATHRSGLRPDGSGFALAELGVRLDGDA
                     PFSPDFIVPGQLGSGLADTGHVMYLRGLKVEALPGTEVLSTTLKPFFNREWKHFCSHR
                     HTPADDAADYPGAVRNGRCIYLMHPLFTLYDRLGPLWCKRLVTNALDLLLPDPLVRAG
                     GPSTALVTVTEQAAQS"
BASE COUNT          204 a          493 c          366 g          209 t
ORIGIN      
        1 atatctgcat cccaaccgcg tgtagctgtc cggcctgcgt ccgctcgatg gacgaggccg
       61 gactcgaccc cgccaacgct cagcaccgtg ccgactttgg gttcgaggtg ctccggcgat
      121 tccaggaccg gctcttcgcc accgtgcggt cccatcaccc cgaggccacc gtgttcttca
      181 actcgggcca cgtcggcccc aagcaccgcc ggataatcga tgcgttcacc catctcgaac
      241 tcgagtcgct gcccagtggc ggttggggct acatgcactt cccactggcc cagcgcttcg
      301 cccggggcct cgaccgcgac accatgggca tgaccggcaa gttccatacc agttggggcg
      361 acttccagtc gttcaagaac cccgccgccc tcgacttcga atgcctcaac atgctcgccc
      421 tcggagccaa gtgcagcatc ggcgaccagc tccacccccg gggacgcatc tgcaaagaca
      481 cctacgccct catcggccgc acctatgcca aagtcgaagc cgccgagccc tggtgcgtgg
      541 gcgcgaagcc tctatcggac atcgctgtgc tcacccccga ggagttcgcc agcgggcata
      601 gccacgcggg actccccaac gaagcgctcg gcgccgcgcg catcctgcag gaatcgcgcc
      661 accagttcga cctggccgat tcccacaccg acctggccgc ataccgggtc gtcatcctgc
      721 ccgacagcat ccccgtggac tccggcctcg ccgccaaact ctccgcgttc gtggcccacg
      781 ggggcgcgat cctggcgacg caccgctctg gcctccgccc cgacggctcg ggtttcgcac
      841 tggccgagct cggcgtccga ctcgacggcg acgcgccctt ctcgcccgac ttcatcgtgc
      901 cgggtcagct cggatcgggg ctggccgata cgggccatgt gatgtacctc cggggtctca
      961 aggtggaggc gctgcccggc accgaggtgc tctccacgac gctgaagcca ttcttcaacc
     1021 gcgagtggaa gcacttctgc tcgcatcgcc acacgccagc cgatgacgcc gctgactatc
     1081 ccggcgccgt gcgaaacggc cgctgcatct acctcatgca cccgctgttc acgctatatg
     1141 accgcctcgg gccactctgg tgcaagcgcc tggtgacgaa cgccctcgat ctgctgctcc
     1201 ccgatcccct cgtgcgcgcc ggcgggccca gcacggcact ggtaacggtg accgagcagg
     1261 cggcccaatc gc
//
LOCUS       DAOSPL010001514         1272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_108023_length_1272_cov_2.791290, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001514 DAOSPL010000000
VERSION     DAOSPL010001514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108023_length_1272_cov_2.791290"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..597)
                     /locus_tag="PLQ54_21045"
     CDS             complement(145..597)
                     /locus_tag="PLQ54_21045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOS95808.1"
                     /translation="MDMVISSALDYAYRASLILARNYDDGGPVTTAQIASAEGLSEGF
                     LLQVLAILRRAGIVHSIRGKNGGYRLAKAPSQVTLADICGAMQTSRPRSDCPMRPDAK
                     CCRNDPTCHLVHALSEASRAAEARLAAFDLETLSQADDSEAQAVLYAS"
     gene            850..>1272
                     /locus_tag="PLQ54_21050"
     CDS             850..>1272
                     /locus_tag="PLQ54_21050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95809.1"
                     /translation="MNPRERVLAAMELQQTDRTPRDFWAEDATLSQLLSYTGSTDTET
                     LLRQMGVDIRHTTAGMPPERWVQGAYQNAWGERYRYEPTPWGPARSALPGALSTARSF
                     SDLEQFRWPSVDELDFSDLSAQCEAWSDYAIVYGFADIW"
BASE COUNT          237 a          439 c          395 g          201 t
ORIGIN      
        1 ggcgaggaag taccacggcc ttcgcgatgg gagagctgat ccctccgccg gccttctggc
       61 tcagtggggc caatgagaac ggcaggcccc gctcggggcc cgccgccacc tgccaatgaa
      121 agggtccgcg gagccaggct cacgctaact ggcgtacaga accgcctgcg cctcgctgtc
      181 gtccgcctgg ctcaatgtct ccagatcaaa cgcggccagc cttgcttcgg cagctcgact
      241 cgcctcggac agtgcatgaa cgaggtgaca cgtcggatcg ttccggcagc acttggcgtc
      301 cggacgcatc gggcaatcag accgtggtcg gctcgtctgc atcgcgccgc agatgtcggc
      361 cagcgtgacc tgggaaggtg ccttggcgag acgatagccg ccgttctttc cccggatgct
      421 gtgcacgatc cccgcgcggc gcaggatggc cagaacctga agcagaaacc cctccgacaa
      481 cccctcggct gacgcaatct gggccgtggt caccggcccg ccgtcatcgt agttgcgggc
      541 taggatcaat gaggcccggt aggcgtagtc cagagccgat gagataacca tgtccatctc
      601 caacctgatc ggaaccgttg atgagtagct cggagcacac acctgacccc ggccgctggc
      661 cgggcatcaa cggggtgcca gccctcgcgc cacccaccga gtggcgcggg aaggcccaag
      721 catccctcct tccgccgacg tgtaccgcag accagcggaa tagacgcatg gtgctacgaa
      781 ttgttcacat cgacacagat tcttagtaca cgaaactcca gatcgcccct cggcaccccg
      841 gaggtcgtca tgaacccgcg cgaacgcgtc ctggccgcca tggaactgca acagacggac
      901 cgcaccccca gggacttctg ggccgaggac gccacgctga gccagcttct gtcctacacg
      961 ggctccacag atacagagac gttgctgcgc cagatgggtg tcgacatccg gcacaccacc
     1021 gccgggatgc cgcccgaacg ctgggtccag ggagcgtacc agaacgcgtg gggcgagcgc
     1081 taccggtacg agccgactcc atgggggccc gcccggagcg cactgccggg agccctcagc
     1141 accgcgcggt ccttctccga tctcgagcag ttccgctggc cgtcggtcga tgagctggac
     1201 ttctcagacc tgtcggccca atgcgaggcg tggagtgact acgccatcgt ctacgggttc
     1261 gccgacatct gg
//
LOCUS       DAOSPL010001515         1271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_108118_length_1271_cov_3.300987, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001515 DAOSPL010000000
VERSION     DAOSPL010001515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108118_length_1271_cov_3.300987"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /gene="pyk"
                     /locus_tag="PLQ54_21055"
     CDS             complement(<1..782)
                     /gene="pyk"
                     /locus_tag="PLQ54_21055"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOS95810.1"
                     /translation="MRRTKIICTLGPACSDDATLRAMLRAGMDVARLNFSHGTHDEHR
                     AMSARVRQLAEEMCRTLAIMMDLQGPKIRTGRLAAGPVELVPGATFTITTDDVPGDAQ
                     CVSTTYDRLPQDVNAGDRILLADGAMELRAVSKTETSVVCEVVYGGLLDEHKGINLPG
                     VAVSAPPLTDKDRRDLALATELGADWIALSFVRSANDIRVIRAALTQLGCDARVVAKL
                     EKPEGIGELDEILSLADAVMVARGDLGVELSPEQVPVLQKRI"
     gene            complement(811..>1271)
                     /locus_tag="PLQ54_21060"
     CDS             complement(811..>1271)
                     /locus_tag="PLQ54_21060"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOS95811.1"
                     /translation="QCTRLISLPIAKDHMIAGVTGAMKNYFGCIASPNKYHADVCDPY
                     VADLFSHRVFRSRHALAVCDAATLLYEGGPHDSPDHHYAYDGIIAGLDPVATDRVIWA
                     IIEGVRAEKGLGPLIDDGRSPTYLASASALGLGCCDFESIQVVQEELPGR"
BASE COUNT          200 a          387 c          472 g          212 t
ORIGIN      
        1 atgatgcgct tctggagcac cggcacctgc tcgggcgaga gttcgacccc gaggtcgccc
       61 cgggccacca tgacggcatc ggccagggag aggatctcgt cgagttcgcc gatgccctct
      121 ggcttctcga gcttggccac gacgcgggcg tcgcagccca gctgggtgag cgcggcgcgg
      181 atgacacgga tgtcgttggc cgaacggacg aaggagaggg cgatccagtc ggcgcccagc
      241 tcggtcgcga gtgccaggtc gcggcggtcc ttgtcggtca gcggtggcgc gctgacggcg
      301 acaccgggca ggttgatgcc cttatgctcg tcgagcagcc cgccgtagac gacctcgcac
      361 acgacggagg tctctgtctt cgatacggcc cggagttcca tggcgccatc ggccagcagg
      421 atgcggtcgc cggcgttgac gtcttggggc agccggtcgt aggtggtcga gacgcactgg
      481 gcatcgccgg gcacatcatc ggttgtgatg gtgaatgtgg cccccggcac cagctcgacg
      541 gggcctgcgg caaggcggcc ggtgcggatc ttgggcccct gcaggtccat catgatggcc
      601 agagtgcggc acatctcctc ggccaattgg cgcacgcgcg cggacatggc gcggtgctcg
      661 tcgtgggtgc cgtgcgagaa gttgaggcgc gccacatcca tgcccgcgcg aagcatggcc
      721 cgcagggtgg cgtcgtcgga gcaggcgggc cccaaggtgc agatgatctt ggttcgtctc
      781 atggcggtcc cgtccggaac gagatgggca tcagcggccc gggagttcct cctgaacaac
      841 ctggatggac tcgaagtcgc agcagccgag gcccagggcc gacgcgctgg caaggtacgt
      901 cggggaccgc ccgtcgtcaa ttaagggacc gagccccttc tcggcgcgca ccccctcgat
      961 gatggcccag atgacccggt cggtggcgac ggggtcgagg cccgcgatga tgccatcgta
     1021 cgcatagtgg tggtcggggc tgtcgtgggg gcccccctcg tagaggaggg tggctgcatc
     1081 gcagactgcc agagcgtggc ggctccggaa gacgcggtgg ctaaacagat cagccacgta
     1141 ggggtcgcag acgtcggcgt ggtacttgtt ggggctcgcg atacagccaa agtagttctt
     1201 catggcgccg gtgaccccgg cgatcatgtg gtccttggcg ataggcagtg agatgaggcg
     1261 cgtgcactgc t
//
LOCUS       DAOSPL010001516         1270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_108257_length_1270_cov_3.050206, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001516 DAOSPL010000000
VERSION     DAOSPL010001516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108257_length_1270_cov_3.050206"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1270
                     /locus_tag="PLQ54_21065"
     CDS             <1..>1270
                     /locus_tag="PLQ54_21065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95812.1"
                     /translation="PAAGVWFYWNQDDRGMRVVVRQAGQYRVIYGAPFAWKPGEAHTV
                     AFTWGEGGSGAIYVDRQRLAEVPAGSCLDAGTDLSRAVLSLGDADSGFTVRQVRMSSE
                     PLGVEQLGTDDDVLEPLPSTLLLDRFDPVRSAGGQRTTTPARSASGAVGTLGIGVMQT
                     EAGLDLSGAGRKGTLLDSLRDQGIRTIGFHEHWTDWQGFPRTNHAEELRSLIAGCHQS
                     GLKLILYHSWQLSDTAPEYPLYLSECEVIAPDRFIYTREPKQRDYPTCARSAWADFLA
                     DGIQKLFEQFAPDGIYSDGLAYPTECANTLHGCGYIGEDGKVHPTFSLFAMRDAVKRF
                     AKILEDQGKPTLFVCHTSGSVTLPTLSFCDAYLDGEHLTGQARPFRVPLDSFRAEFMG
                     HNFGIPAYFLVYDWNQGMTTPEGLAISLLHD"
BASE COUNT          207 a          452 c          403 g          208 t
ORIGIN      
        1 cccgccgccg gtgtgtggtt ctactggaac caggacgacc ggggcatgcg cgtggtggtc
       61 cgccaggcgg ggcagtatcg cgtcatctac ggggcgccat tcgcctggaa gcccggcgag
      121 gcccacacgg tggccttcac gtggggagag ggcggatccg gcgccatcta cgtcgaccgg
      181 cagaggctgg ccgaggtgcc cgccggctcg tgcctcgacg ccgggaccga cctctcgcgc
      241 gccgtcctct ctctcggcga tgccgatagc ggcttcaccg tccgccaagt ccgcatgtcg
      301 tcggagccat tgggcgtgga gcagctcggc acggacgacg acgtgctgga accgcttcct
      361 tcgacactcc tactcgaccg cttcgacccc gtcaggagcg cgggcggtca gcgcacgacg
      421 acgccggcgc gcagtgcctc gggagccgta ggtaccctag ggatcggcgt gatgcagacc
      481 gaagcgggac tcgacctgtc tggtgcgggc cggaagggca ccctcctcga ttcgctccgt
      541 gaccagggca tccgcaccat tgggttccac gagcactgga cggactggca aggattcccg
      601 cggaccaacc acgccgagga gctccggtcg ctgatcgccg ggtgccacca gtccggcctc
      661 aaactcatcc tctaccactc gtggcagctg tcggacaccg cacccgagta cccgctctac
      721 ctgtccgagt gcgaggtcat cgcgcccgac cggttcatct acacgcgcga gcccaagcag
      781 cgcgattacc ccacctgcgc cagaagcgcg tgggccgact tcctggccga tgggatacag
      841 aagctgttcg agcagttcgc gccggacggc atctactcgg acggcctggc ctatccgacc
      901 gagtgcgcca acacactgca cggctgcggc tacatcggcg aggacggcaa ggtacacccc
      961 accttcagcc tgttcgccat gcgcgatgcc gtgaagcgct tcgccaagat cctcgaggac
     1021 cagggcaagc ccacgctgtt cgtgtgccac acctcgggca gcgtcacgct gcccacgctc
     1081 tcgttctgcg atgcctacct cgacggcgag catctgacgg ggcaggcgcg accgtttcgg
     1141 gtccctctcg actcgttccg cgccgagttc atgggccaca acttcggtat tccggcctac
     1201 tttctggtct acgattggaa ccaggggatg accacacccg agggcctggc catctcgctg
     1261 ctgcacgaca
//
LOCUS       DAOSPL010001517         1269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_108393_length_1269_cov_2.411038, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001517 DAOSPL010000000
VERSION     DAOSPL010001517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108393_length_1269_cov_2.411038"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..>1269
                     /locus_tag="PLQ54_21070"
     CDS             71..>1269
                     /locus_tag="PLQ54_21070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95813.1"
                     /translation="MALICACVLASMGAGDAQPGPITLAELAERSTPGVSVAEVTDYA
                     PGHYTGPMEPSPPHADGNARRAVVVYWRDRAERLIFSHEASYCPLLELPSGAAMCNQF
                     FEGNLGDAELFNNPGRRERNSFVDVIKAGPERVWVRWTYFCVNMNDDTRPRLRGTEDY
                     FAAPNGLVLRRMSYESLMPGEVVGYSTDPVELFGVAPVGATISDLFPRDPAHGDYQTH
                     TVLDLFSNERYDIYWCENGGVRRRGDDATLAAISRSRGCALVMPFREKLLFAILGEAS
                     GFPPDRNQLVDHCTPGAEGGCGWGTGLWDHWPIGWLNSQTNVWKPGSPYPYSFGSVGQ
                     FFVPPGQRIRSFSPDYFELCKDMELNRWTERRVFAVLLGAAGSWAEVRRIGRAWLDQG
                     ARCMEPE"
BASE COUNT          195 a          430 c          432 g          212 t
ORIGIN      
        1 ttacctccag gggcctccac tgcactacaa tgcccctgag tgagacggag tatgatggag
       61 gcgttccccc atggcactca tctgcgcgtg cgtgctggct tccatgggtg ccggcgatgc
      121 ccagcccggg ccgatcactc tggccgagct ggcagagcgg tcgacgccgg gcgtctctgt
      181 tgccgaggtc accgactacg cacccggcca ctacaccgga cccatggagc ccagccctcc
      241 gcatgccgat ggtaacgcgc gcagggccgt ggtcgtctac tggcgcgacc gggcggagcg
      301 gctcatcttc agccacgagg ccagctactg cccactcctc gagctgccga gtggcgccgc
      361 catgtgcaac cagttcttcg agggcaatct gggcgatgcc gagctgttca acaaccccgg
      421 ccggcgcgag cggaacagct tcgtcgatgt tatcaaggcc ggcccggagc gcgtgtgggt
      481 ccgctggacc tacttctgcg tgaacatgaa cgacgatacg cgcccgcgcc tgcgcggcac
      541 cgaggactac ttcgccgcgc cgaacggcct ggtgctgcgg cggatgagct atgagagcct
      601 catgcccggc gaggtggtcg gctacagcac tgacccggtc gagctgttcg gcgtggcgcc
      661 ggtgggcgcc accatcagcg acctgttccc gcgcgacccg gcgcacggcg actaccagac
      721 ccacacggtg ctggatctct tcagcaacga gaggtacgac atctactggt gtgagaacgg
      781 gggcgtccgc cggcggggcg atgacgccac gctcgccgcc atctcgcgct cgcgcggctg
      841 tgcactggtg atgccgttcc gggagaagct gctgttcgcc attctgggtg aggccagcgg
      901 gttcccgccc gaccgcaatc agctcgtcga tcactgcact cccggcgccg agggcgggtg
      961 cgggtggggc accggcctgt gggaccattg gcccatcggc tggctcaact cacagaccaa
     1021 cgtctggaaa cccgggtcac cctatccgta cagctttggg tcggtcggcc agttcttcgt
     1081 cccaccgggc cagcgcatcc ggtcgttctc cccggactac ttcgagctat gcaaggacat
     1141 ggagctgaat cgctggaccg agaggcgcgt gttcgccgtg ctcctgggcg cggcggggag
     1201 ctgggctgag gtgcgtcgca tcggccgcgc gtggctcgac cagggcgcgc ggtgcatgga
     1261 gcccgagag
//
LOCUS       DAOSPL010001518         1266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_108725_length_1266_cov_3.464079, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001518 DAOSPL010000000
VERSION     DAOSPL010001518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108725_length_1266_cov_3.464079"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..>1266)
                     /locus_tag="PLQ54_21075"
     CDS             complement(223..>1266)
                     /locus_tag="PLQ54_21075"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HOS95814.1"
                     /translation="WTEWELVRHATARFPGHRQPRVPSWGYVDEADPKVMEAKIDVAA
                     DHGIDYWIFDWYWYDDGPYLSRCLEEGYLRASNSQRVRFCCMWANHDWIDIHPAKYVD
                     PRRLLYPGRVTPQTFGTMVEHILNTYFAHPAYFTVEGRPYFSIYELEKLVESFGGVAQ
                     TREALDHFRARTRAVGFPDLHLNAVVWGQPVLPGEKTPADPRRLLADLGFDSFTSYVW
                     VHHVPLHEFPETDYNHVRDCYFEYWDRAVGEIPLPYFPNATMGWDSSPRTVQSEVFRP
                     VGYPFTPLIGGNTPERFREALRMVRDRLDARPAPRVLNINAWNEWTEGSYLEPDTITG
                     LAYLRAVRDVFAG"
BASE COUNT          212 a          406 c          430 g          218 t
ORIGIN      
        1 ccggcgccct gccagtcggg gtactgcgac gcgaacatgc tcatgacctc ttcgcatggc
       61 gccgggtgcg aataggtcat gagcatgttc gcgtcgcagt accccgactg gcagggcgcg
      121 gtcaccggct cctccatgtg gaccgatgct ctcggctggc gggtggaggt cctggagtga
      181 gctcgggcgc ctccggtacg ccccaaccgg atcctgagcg cctcagcccg cgaacacgtc
      241 ccggaccgcc cgcaggtagg cgaggcccgt gatcgtgtcg ggctccaggt agcttccctc
      301 ggtccactcg ttccatgcgt tgatgttcag gacccgtggg gctggacgcg catcgagccg
      361 atcgcgcacc atgcgcagcg cctctcggaa gcgctcgggc gtgttcccac cgatgagtgg
      421 cgtgaaagga tagcccaccg gccggaagac ctcggactgc accgtccgcg ggctcgagtc
      481 ccagcccatg gttgcattcg ggaaataggg cagggggatc tcgccaacgg cccgatccca
      541 gtactcgaag tagcagtctc gcacgtggtt gtagtcggtc tcggggaact cgtgcagcgg
      601 gacgtggtgc acccagacat acgacgtgaa gctgtcaaag cccaggtcgg ccagcagccg
      661 cctgggatcg gcaggcgtct tctcaccagg cagcaccggc tgcccccaaa cgacggcgtt
      721 caggtgaagg tccgggaagc ccactgcgcg agtgcgcgcc cggaagtgat cgagtgcctc
      781 gcgcgtctgg gcgacacctc cgaagctctc gacgagcttc tcgagttcat agatcgagaa
      841 gtaggggcgt ccctcgaccg tgaaataggc cgggtgcgcg aagtaggtgt tcaggatgtg
      901 ctcaaccatg gtgccgaatg tctgtggcgt cactcggccc gggtagagca ggcgccgcgg
      961 gtcgacgtac ttggccgggt ggatgtcgat ccagtcgtgg ttggcccaca tgcagcagaa
     1021 gcgcacccgc tggctgttgc tcgcgcggag atagccctcc tcgagacaac ggctcaggta
     1081 cggtccgtcg tcgtaccagt accagtcgaa gatccagtag tcgatcccgt ggtcggcggc
     1141 gacgtcgatc tttgcctcca tgaccttcgg gtcggcctcg tccacgtagc cccaggaggg
     1201 tacgcgcggc tggcggtggc cagggaagcg agcggtggcg tgccgcacca actcccactc
     1261 ggtcca
//
LOCUS       DAOSPL010001519         1261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_109401_length_1261_cov_3.049751, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001519 DAOSPL010000000
VERSION     DAOSPL010001519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109401_length_1261_cov_3.049751"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PLQ54_21080"
     CDS             <1..562
                     /locus_tag="PLQ54_21080"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOS95815.1"
                     /translation="DSDQLPHVLDDVIAGRFTIDERTMVQVHGPGGNGITALNDVVIR
                     RERAPGMIDCDISVGGTLVGRYRGDGVLVSTPTGSTCYSLAASGPVLSPWLNALVIVP
                     VCVFTLNIRPLVIADDEDIAVQVQYKGIESPGVSYSADGLPGVELRPGDVIHVSRAAH
                     PARFVRLETGTFYTRLRQRLRWGAEI"
     gene            complement(559..1173)
                     /locus_tag="PLQ54_21085"
     CDS             complement(559..1173)
                     /locus_tag="PLQ54_21085"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOS95816.1"
                     /translation="MLFLASIGGPFRALQPERSWAEGAIPDYVRPNHPAEGSVTANTA
                     PAVNAATLRTRPERIAACIEGDAKTAAQLVTLVIRQRPQLCDPDAYDISLCIARWSRN
                     YGIRPEIVAALIERESRFNPSAVSATNDHGLMQLHAKRIYSIDENIQAGCCHLAGCLA
                     AAGGNERQALAQYNGGGGYGGRSLAYADSILSRAKEIRSTLLSR"
BASE COUNT          208 a          414 c          417 g          222 t
ORIGIN      
        1 cgacagcgac cagctacctc atgtgctcga tgatgtcatc gcgggacgat tcaccatcga
       61 tgagcgcacg atggtccagg tgcacgggcc cggagggaac ggcatcacgg ccctcaacga
      121 tgtcgtcatt cggcgggagc gagccccggg gatgatcgac tgcgacatct cagtgggagg
      181 gacgctggtg ggccggtacc gtggcgacgg cgttctggtc tcgaccccca ccggctcgac
      241 atgctactca ctggccgcat cgggacccgt gctctccccc tggctcaatg ccctcgtcat
      301 cgtgcccgtg tgcgtgttca ccctgaacat ccggccactg gtcatcgccg acgatgagga
      361 catcgcggtt caggtgcagt acaaaggcat cgagagccca ggggtatcct atagcgcgga
      421 tggcctgccc ggtgtcgagc tgcggcccgg cgacgtcatc catgtgagcc gggcagccca
      481 tcctgcgcgg ttcgtgcggc tcgagaccgg cacgttctac acgcgtctgc ggcagcgcct
      541 ccggtggggc gccgagatct agcgcgagag gagggtgctc cgtatctcct tcgctcggct
      601 aagtatcgaa tcggcgtatg cgagcgagcg ccctccgtag ccgcctcccc cgttgtactg
      661 cgccagcgcc tgccgctcgt taccgccggc cgcagccagg caccccgcca gatggcagca
      721 tccggcctgg atgttctcgt cgatcgagta gatccgcttg gcatgcagct gcatcaggcc
      781 atgatcgttc gtcgcgctga cggccgacgg gttgaaccgg ctttcgcgtt cgatgagggc
      841 cgcaacgatc tcagggcgga tcccgtagtt gcgcgaccac cgcgcgatgc acaacgagat
      901 gtcgtacgca tccgggtcgc acagctgggg ccgctggcgg atcacgagcg tcaccagctg
      961 ggccgcggtc ttggcgtcgc cctcgatgca ggccgcgata cgctccggac gagtgcggag
     1021 tgtggccgcg ttcacggccg gcgccgtgtt ggcggtcacg gatccctcag ccggatggtt
     1081 gggtctcacg tagtcgggga tagccccctc ggcccaggac cgctctggtt gcagggcacg
     1141 gaacgggccg ccgatcgagg caaggaataa catgctcacg gcaactccgc ccaagcttcg
     1201 aggacagaga agcgctctca ggaatctcgc ttgaccatat gtggcacaac tcatggacgg
     1261 t
//
LOCUS       DAOSPL010001520         1260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_109553_length_1260_cov_2.424896, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001520 DAOSPL010000000
VERSION     DAOSPL010001520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109553_length_1260_cov_2.424896"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PLQ54_21090"
     CDS             <1..562
                     /locus_tag="PLQ54_21090"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HOS95817.1"
                     /translation="GALVWALIRRRQSLWAAAQALARLPGRVAAALSALLSRWMGGRG
                     RTPRGPRDRGVRTPVYTNPFTHSAEAASMSAHELIAYTYGALMAQADRLGTPRRADQT
                     ALEFASDLPQPLRPLRGEIRDLTRMYVEAEYAEGTDLSRHRERLEQIWCRLDDLAAGV
                     AGVRDSQRHRHPVGGEDGRTPHPAER"
     gene            595..>1260
                     /locus_tag="PLQ54_21095"
     CDS             595..>1260
                     /locus_tag="PLQ54_21095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOS95818.1"
                     /translation="MGLNLVQKILGEHLVAGELKPGAEIAIRIDQTLTQDATGTMAYL
                     QFEAMGIPRVRTECSVSYVDHNTLQTGFENADDHLFLQDAAARFGVWFSRPGNGICHQ
                     VHLERFAVPGRTLLGSDSHTPTCGGLGMIAMGAGGLDVACAMAGEPYALTMPAVVRVH
                     LTGELGPWVSAKDVILELLRRETVRGGVGRIYEYDGPGVATLSVPERATITNMGAELG
                     ATTS"
BASE COUNT          202 a          409 c          449 g          200 t
ORIGIN      
        1 cggggcactc gtctgggccc tcatccgccg gcgccagtcg ttatgggcgg cggcgcaggc
       61 gctggctcgg cttccgggcc gggtggcagc ggccctgagc gcgctcctga gccgatggat
      121 ggggggacga gggaggacgc cgcggggccc gcgggatcga ggggtccgca ctccggtcta
      181 caccaaccct ttcacccact cggccgaggc ggcatccatg tccgcccacg agctcatcgc
      241 gtatacttac ggggcgctga tggctcaggc cgaccggttg ggcacacccc gcagagccga
      301 ccagacggca ctggagttcg cgagcgactt gccccagccg ctccgcccgc tgcgaggcga
      361 gatccgggac ctgacccgca tgtatgtcga ggccgagtac gccgaaggca cggacttgtc
      421 gcggcaccgt gagcgcctcg agcagatatg gtgccgcctg gacgacctcg ccgccggcgt
      481 cgcgggagtg cgggactccc agagacaccg gcatccggtt ggcggcgagg acgggcgcac
      541 tccacaccca gccgagcgtt gaccattggg gctgctgcga gaaggtggat taccctggga
      601 ctcaatctgg ttcagaagat actcggtgag catctcgtcg ccggcgagct caagcccggg
      661 gctgagatcg ccattcgcat cgatcagaca ctcacgcagg atgccacggg aaccatggcc
      721 tacctgcagt ttgaggccat gggcataccg cgcgtgcgga ccgagtgctc ggtcagctat
      781 gtcgaccaca acacgctcca gaccggcttc gagaacgccg acgatcactt gttccttcag
      841 gacgcggcgg cgcgctttgg cgtgtggttc tctcggccgg gtaacggcat ctgtcaccag
      901 gtccatttgg agcgattcgc cgtgcctggc aggacgctgc tcgggtcgga cagccatacc
      961 cccacctgcg gtggcctggg catgatcgcc atgggcgccg gtggcctgga cgtggcgtgt
     1021 gccatggcgg gagagccgta cgcgctgacc atgccggctg tggtccgcgt gcatctgacc
     1081 ggggaactcg gcccgtgggt atcggcgaag gatgtgatcc tcgaactgct ccggcgcgag
     1141 acagtgagag gtggggtcgg gcggatctac gagtatgacg gccccggggt ggcgacgctg
     1201 agcgtgcccg agcgtgccac gatcaccaac atgggcgccg agcttggggc cacgacatcg
//
LOCUS       DAOSPL010001521         1258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_109777_length_1258_cov_2.839568, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001521 DAOSPL010000000
VERSION     DAOSPL010001521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109777_length_1258_cov_2.839568"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PLQ54_21100"
     CDS             <1..295
                     /locus_tag="PLQ54_21100"
                     /inference="COORDINATES: protein motif:HMM:NF018767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HOS95819.1"
                     /translation="EGHEALIVLNTGDEDARIAVDVYFSDREPETGIQLVAPARRVTC
                     FRLDKPLGDRQFRVPFGQYALRLRSSAPIVVQMGRADVRQPNLAYYMNIGHAE"
     gene            complement(439..591)
                     /locus_tag="PLQ54_21105"
     CDS             complement(439..591)
                     /locus_tag="PLQ54_21105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95820.1"
                     /translation="MPRLESPSRDELVALWVGLHPRTEELSAENARLRAALEAKRGGG
                     EPSRAA"
     gene            1060..>1258
                     /locus_tag="PLQ54_21110"
     CDS             1060..>1258
                     /locus_tag="PLQ54_21110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95821.1"
                     /translation="MGFHELVPAEARKQPKLPPRRTAFAHQLDAVKALDRAFDLDSKR
                     GVGALLVLPTGAGKTYTTVSWL"
BASE COUNT          200 a          450 c          418 g          190 t
ORIGIN      
        1 cgagggacac gaggcgctca tcgtcctgaa cacgggcgat gaggatgcgc gcatcgcggt
       61 ggacgtctac ttctcggacc gcgagccgga gacgggcatc cagctcgtgg cgccggcgcg
      121 tcgcgtgacg tgcttccgcc tggacaagcc cctgggcgac cgccagttcc gcgtgccctt
      181 cggccagtac gcgttgaggt tgcggtcgtc cgcaccgatc gtcgtgcaga tgggccgggc
      241 cgacgtgcgg cagcccaacc tggcctacta catgaacatc gggcatgccg aatagccgag
      301 tggtccccgt cgatccgtca ggttcccgag tcgggcccgg ccggcaggca gcagtcgagc
      361 acgcatcggg cgcaccgagt ggtacccatc gtcgaggcac tcccgtgcca cccgatcggg
      421 cttcactcac gccgacgcct acgcagctct gctgggctcc ccgcccccac gcttggcctc
      481 cagcgctgcc cgaagccgag cgttctcagc cgacagctcc tcggtgcgcg ggtgcagtcc
      541 cacccacagc gcgaccagct cgtctcggct cggggactcc agacgtggca tgacccttcc
      601 ctacgacatc aacctcaccc gtaccggccc ggatcctcat tgagtcgtgg cgcttgcgac
      661 cctcgtgtag cgccggccgc gtgggttcct cgggactccg gatggccggc cggcaagagg
      721 cccgtgtcca ccgcccttcg tcacgccgcg tggacgcggc ggctcagggt cggcgggttg
      781 ggcgtgaagg cggcgacgcc cgagcagccg tcgaggaccg cttgagagtt ggctcagagc
      841 aaaggggatt cgcaaccgcc ggcacagcgc gctcccagtt cggaagtaac acgcctctgc
      901 aggagtcgac cgccgatagg ccgaagaacc gaccaacagc ccgtgggtgc ggcactgggg
      961 gggctgggcg ggttataccg accgggctcg acctagcgcg tgcagcgaac gctgcacggc
     1021 gcttacggtc acgagcttgc actggaggca tgtgttcgca tgggtttcca cgaactggtc
     1081 ccggccgagg cacgcaagca gccgaagctg cccccccgcc gcaccgcctt cgcccaccaa
     1141 ctcgacgccg tcaaggctct cgacagagcg ttcgacctgg acagcaagcg aggagtgggt
     1201 gccctcctcg tcctcccgac cggggcgggg aagacctaca ccacggtcag ttggctct
//
LOCUS       DAOSPL010001522         1252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_110707_length_1252_cov_1.705931, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001522 DAOSPL010000000
VERSION     DAOSPL010001522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110707_length_1252_cov_1.705931"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..168)
                     /locus_tag="PLQ54_21115"
     CDS             complement(19..168)
                     /locus_tag="PLQ54_21115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95822.1"
                     /translation="MRRLRRRLRHLRRDIRMWRDVAHPAIGYTAFAAIILATAGLVTY
                     ALTTH"
     gene            449..>1252
                     /locus_tag="PLQ54_21120"
     CDS             449..>1252
                     /locus_tag="PLQ54_21120"
                     /inference="COORDINATES: protein motif:HMM:NF019842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PPE domain-containing protein"
                     /protein_id="HOS95823.1"
                     /translation="MVLAATVVPAPDPTLSQGVRLSAASTALIMGGTMTPTWRDADVD
                     AIMNQFITPTHPAPPGQTIEPVALTTPEEAWPLTGLFRLISLLTGPSTTYGPGGAGWP
                     DEPWWKLTGLFDLAYDKSVQAGLADLEAAMAKYDNDHLVIYGFSQGAEIAILEKRKLD
                     EQYPDGTEPPDIDFVLSGDFNLPNGGAAARLPGFYIPILDFSFNGAEPTGTQFHTDVI
                     TRQYDLIAEFPLYPLNVIADVNAVLGFFYVHTHAFDVSLASDLSTPPPVK"
BASE COUNT          221 a          407 c          388 g          236 t
ORIGIN      
        1 gtagctggtc cgagctcgtc aatgcgtggt cagggcgtac gtgaccaaac ctgccgtcgc
       61 caggatgatg gccgcgaatg ccgtgtaccc gatggccgga tgtgcgacgt cccgccacat
      121 gcggatatcg cggcgcaggt gcctgagccg tcggcgtagg cgccgcatct gacggtcccc
      181 cgaagattcc atggctctgt ctaccgcgtg gagctgtggg aacgctgatg gtgcgatgtc
      241 gggagcgtta cctcggatga acgaatccgc gccgcggatc agtagtgcta tctcctgggc
      301 cccgcgaaac gaagtaggcc gctactgcac tcatcactgg ccgtgcgact gacgattcca
      361 ttgcggactt cgttgcgcat tggcatgagg agtcacgatg atgggttatt ggaagcatgt
      421 agatcggttc gggacagttg ccgtagccgt ggtgctggcc gccacggtgg tgccggcgcc
      481 ggaccccacg ctgtcgcaag gtgtgcgact ttccgcggca tcgaccgcgt tgatcatggg
      541 cgggactatg accccgacct ggcgcgacgc cgacgtcgac gccatcatga atcagttcat
      601 cacaccgacc cacccggcgc ccccgggcca gacgattgaa ccggtcgcgc tcaccacgcc
      661 cgaggaggcc tggcccctta ccgggctctt tcgcctcatc agcctcctga cagggccctc
      721 gaccacgtat gggcccggcg gtgcgggatg gcccgacgag ccgtggtgga aacttacggg
      781 actcttcgac ctggcctacg acaagtcagt ccaggccggg ttggccgatc tggaagcggc
      841 gatggccaag tacgacaacg accatctggt gatctacggc ttctcgcagg gcgccgagat
      901 cgcgatcctg gagaagcgca agctcgacga gcagtacccg gacggaaccg agcctcccga
      961 catcgacttc gtgctgagtg gggacttcaa tctgcccaac ggcggtgctg cagcgcggct
     1021 accgggcttc tacatcccga tcctcgactt ctcgttcaat ggcgccgagc cgaccggaac
     1081 ccagtttcac accgatgtca tcactcggca atatgacctc atcgcggaat tcccgttgta
     1141 tccgctcaac gtcatcgcgg atgtgaacgc ggtgctgggc ttcttctacg tgcacaccca
     1201 cgccttcgat gtcagcctgg cttccgacct gtcgaccccg ccgcctgtca ag
//
LOCUS       DAOSPL010001523         1251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_110800_length_1251_cov_2.184783, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001523 DAOSPL010000000
VERSION     DAOSPL010001523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110800_length_1251_cov_2.184783"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="PLQ54_21125"
     CDS             complement(<1..1161)
                     /locus_tag="PLQ54_21125"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOS95824.1"
                     /translation="MAEPAKGGKDGPFRTLERARDEVRKLKAAGQLPRGGLVVHIDGG
                     VYELQQPFALTAEDSGTTDCPIVYRARPGSEVRVVGGRVVTGWTRVTDAAVLDRLDPA
                     ARGSVLQADLKALGMTDFPAMEAGQSWGASRPGLEVFFGNEPMTLARYPNEGFITIPE
                     VLGTTPQDIRGTRGCMEGIFRYEGDRPQRWTREKDVMLEGYWFWDWADQRLKVKSIDT
                     TERIITLEPTPQHAYGFRNGMYYYAYNLLPELDQPGEWYLDRDSGILYIWPPSPIDGS
                     PVSVSVLPGLVTMQGVRHVTFRGFTFECCQGTGLSIRDAEDVQIAGCVIRNTGAWAVE
                     LSGRDSSVAGCDIYNTADGGVILSGGDRPSLTPANLSVDNCHIWRYGRWNPIC"
BASE COUNT          199 a          429 c          414 g          209 t
ORIGIN      
        1 gcagatgggg ttccaccggc catagcgcca gatgtggcag ttgtcgaccg acaagttcgc
       61 gggcgtcagt gatggccggt caccacccga caggatcacg ccgccatcgg ccgtattgta
      121 gatgtcgcac ccggccacgc tgctgtcgcg ccccgagagc tcaacggccc acgcgcccgt
      181 gttgcggatg acgcacccgg cgatctgaac atcctccgcg tctctgatcg acagcccagt
      241 gccctggcag cactcgaagg tgaacccgcg gaatgtcacg tggcggacgc cctgcatcgt
      301 cacgagcccc ggcagcaccg acaccgagac tgggctcccg tcgatgggcg agggcggcca
      361 aatgtagagg atgccggaat cgcgatcgag ataccactcg cccggctggt cgagctcggg
      421 cagcaggttg taggcgtagt agtacatccc gttgcggaac ccataggcgt gttgcggcgt
      481 gggttcgaga gtgatgatgc gctctgtggt gtcgatgctc ttcaccttga ggcgctggtc
      541 ggcccagtcc cagaaccagt agccctcgag catgacgtcc ttctcgcggg tccagcgctg
      601 tggccggtcg ccctcgtagc ggaagatacc ctccatgcac cctcgggtgc cccggatgtc
      661 ctgcggcgtc gtcccgagca cttcggggat ggtgatgaag ccttcgttgg ggtaacgggc
      721 cagtgtcatg ggctcgttgc cgaagaaaac ctcgagcccg ggtcgcgaag ccccccagct
      781 ctgcccggcc tccatggcgg ggaagtcggt catcccgaga gccttcaggt ccgcctggag
      841 gacgcttccc cgtgccgctg gatcgagtcg gtcgagcacg gcggcatcgg tcacccgagt
      901 ccagccggtc accactcgcc cgccgacgac ccgcacctcg ctgcccggcc gagcgcggta
      961 gacgatgggg cagtcggtgg tgccgctgtc ctcggcggtg agggcgaacg gctgctgaag
     1021 ctcatagacg ccgccgtcga tgtgaaccac caacccgccg cgcgggagtt ggcccgcggc
     1081 cttgagcttc cgcacctcgt cgcgcgcgcg ctccagcgtg cggaagggcc cgtccttacc
     1141 ccccttggca ggctcggcca tacggccgct ccatgcatcg ttgccctggg tcgagacata
     1201 caccttggat gccgtgcgcg ccggcgccgc accgagagac gccatgatca g
//
LOCUS       DAOSPL010001524         1250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_110904_length_1250_cov_2.491213, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001524 DAOSPL010000000
VERSION     DAOSPL010001524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110904_length_1250_cov_2.491213"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1250)
                     /locus_tag="PLQ54_21130"
     CDS             complement(<1..>1250)
                     /locus_tag="PLQ54_21130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95825.1"
                     /translation="LRVGTEAMHVEECLRRYRAADPRTLTYKTLDLTYKPANYYVYGP
                     MADICNPDCYPLMIDADPKMVREVVETARHGAGPRPVTFTYQSGYIEPGEEETPTRKG
                     RAPFPDEMRTMMYYAIGAGARGLWNYIHCTEGQSHGTHEYPELWYAIGQTYRELGRVA
                     PLLGRAHPMELAQSEQAGLWLRTLICGENAMLVVCVDDQYESRPDAFVVPPRTDVTFS
                     LPHVPWMSPRAAWQVTESGFAPLPLAAGDQLTLPELSSVALVLVSADAGLAGGLEAEF
                     AERETERARGLLRLEQARQRDDARLAHLRRTLESQCAAYAVTGEGIGAYGVTPQGFWN
                     PRGTEYSGFEFGVNDQTDGPTMGATWPISAADAGTRLVVYALTTNWGQPAEWTLLDAG
                     GRPVGIAAQAQLPGTTLTVLELTLP"
BASE COUNT          182 a          424 c          437 g          207 t
ORIGIN      
        1 gggagcgtga gctcgagcac ggtcagtgtc gttcccggca gctgagcttg cgccgcgatg
       61 cccactggcc gtccgcctgc gtccagcagc gtccactccg ccggctggcc ccagtttgtg
      121 gtcaatgcgt agacgacgag ccgcgttccg gcgtctgcgg cggagatggg ccatgtggcg
      181 cccatggtgg ggccgtccgt ctggtcgttc acaccgaact cgaaaccact gtactccgtg
      241 ccgcgtgggt tccagaagcc ctggggcgtc acgccgtagg ccccgatacc ctcacccgtg
      301 accgcgtagg ccgcgcactg gctctccagg gtgcgtcgga gatgagccag gcgcgcgtcg
      361 tcgcgctgtc gtgcctgctc gagccttagc agcccgcgcg cgcgctccgt ctcgcgctcc
      421 gcgaactccg cctcgagtcc gccggcgagc ccggcgtccg ccgacaccag aaccagggcc
      481 accgacgaga gctccggcag cgtcagttgg tcgccggcgg ccagtggcag cggagcgaat
      541 ccgctctcag tcacctgcca cgctgctcgg ggagacatcc agggcacgtg gggtagtgag
      601 aacgtcacgt cggtccgggg cgggaccaca aaggcatcgg ggcgggactc gtactggtcg
      661 tcgacgcaca cgacgagcat ggcattctcg ccgcaaatga gcgtccgcag ccagaggccg
      721 gcctgctctg attgggccaa ctccatgggg tgggcccggc caaggagagg cgcgacgcgg
      781 cccagctcgc ggtaggtctg cccgatggca taccacagct cggggtactc gtgtgtgccg
      841 tggctctggc cctcggtgca gtggatgtag ttccagaggc cgcgcgcccc ggcgccgatg
      901 gcatagtaca tcatcgtccg catctcgtcg gggaacggcg cccggccctt gcgcgtgggc
      961 gtctcctcct cgcccggctc gatgtagccc gactggtagg tgaaggtcac cggccgcggc
     1021 ccggcgccgt ggcgggccgt ctcgactacc tcgcgcacca tcttcggatc ggcatcgatc
     1081 atcagcgggt agcagtcagg attacagata tccgccatgg ggccgtagac gtagtagttg
     1141 gccggcttgt aggtcaggtc gagggtcttg taggtgagcg tgcgcggatc ggcggcacgg
     1201 taccggcgca ggcactcctc cacgtgcatg gcttcggtgc ccacacgcag
//
LOCUS       DAOSPL010001525         1248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_111127_length_1248_cov_3.632858, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001525 DAOSPL010000000
VERSION     DAOSPL010001525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111127_length_1248_cov_3.632858"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PLQ54_21135"
     CDS             complement(<1..707)
                     /locus_tag="PLQ54_21135"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS95826.1"
                     /translation="MRLGFRTAGFAAWPIERTLRALKELGYDGVEVCLEIHELCPGTV
                     TPERLSGVGRALAGSGLELASVSYHADGEDLTTRVPNTIRAVEMTCELGCGILIVNAE
                     RADADRRAEQWRALVGRFRRICERAEECQVWVAVEPEPLLLVHGMDDWARLRDEVASD
                     RLAINLDIGHCEITDDVPAAIRRFASHIVHVHFEDIRGKVHQHLVPGEGDMDLSAVVR
                     ALREVDYDGYLTIDLFH"
     gene            complement(724..>1248)
                     /locus_tag="PLQ54_21140"
     CDS             complement(724..>1248)
                     /locus_tag="PLQ54_21140"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOS95827.1"
                     /translation="LRAPGAEAAHVDGVFAAGDALTGPQTLIDAIAGGHTAAARIHEY
                     LSGERTEPLAIPAGRVDKASLHDEETILTRRQRPRMRPASERVRDFDEVSLGFSDAQA
                     MAEAQRCISCVECAACGQCARICIYGAIEVHNGEPTITTQCDGCGLCVELCPQGAIRM
                     VERADTPGIAALVE"
BASE COUNT          188 a          441 c          422 g          197 t
ORIGIN      
        1 atgtggaaca ggtcgatcgt caggtacccg tcgtagtcca cttccctcaa cgcccggacg
       61 accgccgaga ggtccatgtc gccctctccg ggcaccagat gctggtggac cttgccgcgg
      121 atgtcctcga agtggacgtg cacgatgtgg ctcgcgaagc gccgaatagc tgcgggaacg
      181 tcgtccgtga tttcgcagtg gccgatgtcg aggttgatcg ccagccgatc cgacgcgacc
      241 tcatcccgga gccgcgccca gtcgtccatc ccgtggacca gcagcagcgg ttcgggttcg
      301 acggccaccc agacctggca ctcctctgcg cgctcgcaga tgcgccggaa gcgcccgacc
      361 agcgcccgcc actgctcggc gcgccggtcg gcgtcggccc tctcggcgtt cacgatgagg
      421 atcccgcagc ccagttcgca cgtcatctcc acggcgcgga tggtgttggg cacacgggtc
      481 gtcaggtcct ctccgtccgc gtggtagctc accgacgcta gttcgaggcc ggaacccgcc
      541 agagcgcggc ccacgccgga aagccgctct ggcgtgacgg tgcctgggca cagctcgtgg
      601 atctcgaggc acacctcgac gccgtcgtag ccgagttcct tcagggcgcg gagagtcctc
      661 tcgatgggcc aagcggcgaa gcctgccgtg cggaaaccga gacgcatgga tcagccccct
      721 cccctactcg acgagtgccg cgatgcctgg cgtatcggca cgctcgacca tgcggatggc
      781 cccctgcggg cacagctcga cacacaggcc gcagccatcg cactgggtcg taatggtggg
      841 ctccccgttg tgcacctcga tggcgccgta gatgcagatg cgcgcacact ggccgcaggc
      901 cgcgcactcg acgcagctaa tgcagcgctg ggcttcggcc atggcctggg catcggagaa
      961 gccgagcgag acctcgtcga agtcgcgcac gcgctctgag gccggcctca tccttgggcg
     1021 ctggcgccgg gtgaggatcg tctcctcgtc gtgcagtgaa gccttgtcga cacgcccggc
     1081 ggggatggcg agcggctcgg tccgctcgcc cgagaggtac tcgtggatcc gcgccgccgc
     1141 cgtgtgcccg ccggcgatgg cgtcgatgag cgtctggggc ccggtcagcg catcgccggc
     1201 ggcgaacacg ccatcgacgt gggctgcctc cgcccctggg gcacgaag
//
LOCUS       DAOSPL010001526         1247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_111309_length_1247_cov_2.035235, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001526 DAOSPL010000000
VERSION     DAOSPL010001526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111309_length_1247_cov_2.035235"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..>1247)
                     /locus_tag="PLQ54_21145"
     CDS             complement(222..>1247)
                     /locus_tag="PLQ54_21145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95828.1"
                     /translation="GDPAALSKAIETFADGRQNGATRTRLVERYRLTPQEWEVEAQVR
                     HWGGSDRLFPADVSRAPDRVGDLHMKRLDRPAYRIDFHRGPTPSFPIGRVLAQEVDWQ
                     QESSPCAAYLGLYVRRVGALRRELEAMLQGGGDVAAELTEHDGREAVLLTLTGETAPG
                     FVRQLRCGFVRLGGYAPYLAEVTGISEEGGRQMVVRVFALWEGYVEAPDGTWVPSEYR
                     CSSFSTGASGLPELDSAEAATLVEGTLRATRLPLSDEELAKWFPMGTIVAILPDVRAL
                     LPTSVVDAFERSGRNMGTTKDWLDTDISKLVYRMDEPWYTGTPEERRTLQDALVADVM
                     ARARRAK"
BASE COUNT          187 a          454 c          406 g          200 t
ORIGIN      
        1 gcagattccg tgcgctaaga ttccggcgca cagccacaca ccagtgtccg gatgcttgtt
       61 catgtgagtt ggtcgagccc aacgtagccg tgcgtggggc tcacgccgtg gcccgtgggt
      121 tcgcttcggg cccgtggccg tacgccgcgc ggcgtgcgca atggttcagc tgcgtcagct
      181 cggtcaggcc tcggcacacc atgtccgggc cccccaccgg gtcacttggc ccgtcgggcg
      241 cgtgccataa cgtccgccac cagggcgtcc tgcagcgtgc gccgttcctc gggcgtgccc
      301 gtgtaccacg gctcatccat gcggtacacg agcttcgaga tgtcggtgtc gagccagtcc
      361 ttggtcgtgc ccatgttgcg gccgctgcgc tcaaacgcat ccaccacgct tgtcggcagc
      421 agcgccctga catcgggcaa gatcgccaca atcgtgccca tagggaacca cttcgccagc
      481 tcctcgtcgc tgagcggcag cctggtcgcc cgcagggtcc cctccaccag cgtcgctgcc
      541 tctgccgaat ccagctccgg cagacccgac gccccggtcg agaacgagga gcaccggtac
      601 tcggatggca cccaggtgcc gtccggcgct tcgacgtacc cctcccacag ggcgaacaca
      661 cggactacca tctggcggcc gccttcttcc gagatgcccg taacttcggc aaggtacggc
      721 gcatagcccc ccagcctgac gaacccacac cggagctgac gaacgaagcc cggcgccgtc
      781 tcgccagtca gggtcagcag aacggcctcg cggccgtcgt gctcggtcag ctcggccgcc
      841 acgtcacccc cgccttggag catcgcttcg agctcgcgcc gcagcgcgcc cacgcggcgc
      901 acgtataggc cgaggtaggc cgcgcagggc gaggactcct gctgccagtc gacctcctgg
      961 gccagcactc ggccgatggg gaacgacggc gtggggccgc ggtggaagtc gatccggtag
     1021 gcggggcggt ccagcctctt catgtggagg tcgccgaccc ggtcgggggc acgactgacg
     1081 tcggccggga acagccggtc ggacccaccc cagtgccgca cttgggcctc gacttcccac
     1141 tcctggggag tgagacgata gcgctcgacg agtcgagtgc gcgtggcccc gttctgcctg
     1201 ccatctgcga aggtctcgat ggccttgctg agcgcagcgg ggtctcc
//
LOCUS       DAOSPL010001527         1244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_111654_length_1244_cov_2.890664, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001527 DAOSPL010000000
VERSION     DAOSPL010001527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111654_length_1244_cov_2.890664"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PLQ54_21150"
     CDS             <1..486
                     /locus_tag="PLQ54_21150"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOS95829.1"
                     /translation="HGGVTEATICELSDGRLLMYLRTNWSQFWLATSSNGGLHWHSMG
                     PAGVEASSAPGLLLRLASGRIALFWNRPYPEGKTDWPLSGGDGLWSSVPVSNHRTELC
                     VSFSSDECEHWSAPVVVAKHPKDEWIAYPYALEVEPGMLWVTTMQGDLRCRIHEADFL
                     R"
     gene            681..>1244
                     /locus_tag="PLQ54_21155"
     CDS             681..>1244
                     /locus_tag="PLQ54_21155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95830.1"
                     /translation="MRLRTCHVVAGAAVLVTLSVAAFAQEADTPFGRFAVVDEVVCGD
                     AGDDHAFAQQPENVSTVETLLDQPCRVLPPAGEAKYFAYRLGAGKGLQPGAMYILEVD
                     YPEDVARSVWIENRGCETNLGFATGAAVGDAVVGRYVHLNPESLRYPLSGRYETWRMV
                     FTLHDRFPDLQQPRGEGPRPMLPTEGFW"
BASE COUNT          187 a          420 c          425 g          212 t
ORIGIN      
        1 cacggaggcg tgacggaggc gaccatctgc gagctttcgg acgggcggct gctcatgtac
       61 ctgcgtacca actggagcca gttctggctc gccacgtcga gtaacggcgg cctgcactgg
      121 cactccatgg gaccggcggg cgttgaggcc agcagcgcac ccgggctgct gctccgactc
      181 gccagtgggc gcatcgcctt gttctggaac cgcccctatc ccgagggcaa gacggactgg
      241 cccctctcgg gcggtgatgg cctgtggtcg tcggtccccg tgagcaacca ccggaccgag
      301 ctgtgtgtct ccttctcgag cgatgagtgc gagcactggt ccgcgcccgt ggtcgttgcc
      361 aagcatccga aggatgagtg gatcgcctat ccgtacgctc tggaggtcga gcccggcatg
      421 ctgtgggtga ctaccatgca gggcgatctg cggtgccgga tccacgaggc ggacttccta
      481 cggtgaaagt gggggaagcg cgcggggctc tgggccctcc ggggcgccga atggaacggc
      541 cccccccagg tggcaagggc gctatccggc ccattactgc cttgccgagt ccagggccgg
      601 accgggtccc aacagattgc atctggttca ccccgtttgg cccgtccgaa caccttctga
      661 acaactgagg aggctactcg ttgaggctca gaacatgcca cgtggtcgcg ggtgccgccg
      721 tgctggtaac gctctccgtc gccgccttcg cccaggaggc cgacacgccc ttcgggcggt
      781 tcgctgtcgt cgatgaggtc gtgtgtggcg acgcgggcga tgatcacgcc ttcgcgcagc
      841 agcctgagaa cgtcagcacc gtcgagacgc tcctcgacca gccgtgccgc gtgctgccgc
      901 ctgcggggga ggccaagtac ttcgcctacc gcctcggagc gggtaagggc ctgcagccag
      961 gcgccatgta catcctcgag gtcgactacc cagaggacgt ggcccggagc gtctggatcg
     1021 agaaccgcgg gtgcgagacc aacctgggct tcgccaccgg cgccgccgtc ggcgacgccg
     1081 tcgttggccg ctacgtccac ctgaacccgg agtcgctcag gtacccgctg tcgggccgct
     1141 acgagacctg gcgcatggtc ttcacgctcc acgaccggtt tcccgatctg cagcagccgc
     1201 gcggtgaggg cccgcggccc atgctgccca cggagggttt ctgg
//
LOCUS       DAOSPL010001528         1241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_112111_length_1241_cov_2.175379, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001528 DAOSPL010000000
VERSION     DAOSPL010001528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112111_length_1241_cov_2.175379"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLQ54_21160"
     CDS             <1..242
                     /locus_tag="PLQ54_21160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HOS95831.1"
                     /translation="MRGRGCEACRQRGYRGRTGCYEFMVITDEIREAILKEEPSHVIA
                     EIAREQGMRTLAEDAMLKVAQGITTPEEMLRVCST"
     gene            275..1192
                     /locus_tag="PLQ54_21165"
     CDS             275..1192
                     /locus_tag="PLQ54_21165"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOS95832.1"
                     /translation="METTDALVKRRLELFVQHVDDVPPLPQIAMRVLEMLESPSASAT
                     DLERVILSEQTLAMKILRMANSAFFGLPRSVASLRDAIVFLGFRKVSSIVVGLATFDL
                     FGARRAEETDLFRARLWRHSMLCATCSRRLAESRHDGPPEQAFMIGLLHDIGETILER
                     YARDQFAEVIRLYEETGCPIMDAEAKVLGFNHAELAGALCENWGIPDALTIPIRFHHS
                     PMDAPEEARAAALLCHVANGMAYGLCLEVTEEGPRYVPAEGLTQLPYTDQRAVLALGL
                     DMPRLLLLMEEAGRAALEQESLFRNASAP"
BASE COUNT          211 a          400 c          421 g          209 t
ORIGIN      
        1 tcatgagggg ccgaggctgt gaagcgtgcc gccagcgggg ctaccgcggg cgaaccgggt
       61 gctacgagtt catggtgatc accgacgaaa tccgggaggc aatcctcaag gaggagccgt
      121 ctcatgtgat cgccgagatc gcgcgcgagc aggggatgag gaccctggcc gaggacgcga
      181 tgctcaaggt agcccagggc atcaccacac ccgaagagat gctccgggtc tgctcgacgt
      241 agcccaccgt tctcgctggc tagacgggag tggaatggag acaaccgatg cgctcgtgaa
      301 gcggcggctc gagctgtttg tgcagcacgt cgacgacgtg ccgccactgc cgcagatcgc
      361 catgagagtc ctggagatgc tcgagtcgcc gtcggccagc gctaccgacc tggagcgcgt
      421 gatcctcagc gagcagacgc tggcaatgaa gatccttcgc atggccaact ccgcgttctt
      481 cggcctgccc cggtccgtcg cttcgctccg cgatgccatc gtattcctgg gctttcgcaa
      541 ggtgagctcc atcgtggtgg gactggcgac gttcgacctg tttggcgcca ggcgggccga
      601 ggagactgac ctgttccggg cacgtctctg gcgccactcg atgctgtgcg ccacctgctc
      661 ccgccgtctc gccgagtcca ggcacgacgg ccctccggag caggccttca tgatcggcct
      721 gttgcacgac atcggggaga cgattctcga acgatacgcc cgggatcagt tcgccgaggt
      781 catccgcctc tatgaggaga ccggctgccc catcatggac gccgaggcga aggtgctcgg
      841 gttcaaccat gcggagctcg cgggggctct gtgtgagaac tggggtatac ccgacgcgct
      901 caccatcccc atccgcttcc accacagccc gatggacgcg ccggaggagg ctcgggcggc
      961 ggctctgctc tgccacgtgg ccaacggcat ggcctacggc ctgtgtctcg aagtgaccga
     1021 ggaggggccg cgctacgtgc cggccgaggg cctgacgcag ctaccataca cggaccagcg
     1081 cgcggtgctg gccttgggcc tggacatgcc ccggctcctg ctactgatgg aagaggcggg
     1141 gcgggctgcc ctcgagcagg aatccctgtt ccgcaacgcc agcgcgcctt agggccgccg
     1201 tggagggtca gcggtgcaaa gcattcgaga cgttcacatt g
//
LOCUS       DAOSPL010001529         1239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_112389_length_1239_cov_2.044764, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001529 DAOSPL010000000
VERSION     DAOSPL010001529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112389_length_1239_cov_2.044764"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PLQ54_21170"
     CDS             complement(<1..712)
                     /locus_tag="PLQ54_21170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOS95833.1"
                     /translation="MSEVHPSKQPRQPMPEQPPAERVTNFNEVPLGYTPEQAMAEAAR
                     CLQCKNPRCVTGCPVAVDIPGFIGLTKEGQFVEAARKIMETNVLPAVCGRVCPQETQC
                     EILCVLARRGEPVAIGRLERFVADYEREHGPRETVPPVPATGKRVAIVGSGPAGLTCA
                     ADLARRGHSVTVFEALHELGGVLVYGIPEFRLPKRILKAEVERLRDLGVDLQTSAVIG
                     KLDTIEELLLIREVDPQLF"
     gene            complement(748..>1239)
                     /locus_tag="PLQ54_21175"
     CDS             complement(748..>1239)
                     /locus_tag="PLQ54_21175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOS95834.1"
                     /translation="LRGAGNRLIGIIGARTRGLVILEKELGSLCDRLEVATDDGSYGR
                     KGFVTDALAALIAEGTDIRFVLAVGPTPMMRAVSELTRQYGIPTMVSLNPIMVDGTGM
                     CGGGRVEVDGQTKFACVDGPEFDGHLVNFDLLMKRQGAYRAEERQIAENYEHCRIAGV
                     LDA"
BASE COUNT          205 a          446 c          381 g          207 t
ORIGIN      
        1 agaagagctg ggggtcgact tcgcggatca gcaacagctc ctcgatggtg tcgagcttgc
       61 cgatgaccgc gctggtctgc agatcgactc ccagatccct gaggcgctcg acctcggcct
      121 tcaggatccg cttcggcaac cggaactccg ggatgccgta cacgagcaca ccaccgagct
      181 cgtgcagagc ctcaaacacg gtcaccgagt gcccccggcg ggctagatcg gccgcgcacg
      241 tgaggccagc gggcccggag cctacgatgg caacccgttt tcccgtggct ggcacgggtg
      301 gcaccgtctc gcgcggtccg tgctcgcgct cgtagtcagc cacaaatctc tccagccggc
      361 cgatggccac gggctcgccg cgccgtgcca gcacgcacag gatctcgcac tgggtctcct
      421 gcgggcacac acgcccacac accgccggaa gaacattcgt ctccatgatc ttgcgggcgg
      481 cctcgacgaa ctgcccctcc ttggtgagtc caatgaaccc cggtatgtcc acggccaccg
      541 ggcagccggt aacgcatcgg gggttcttgc actgaaggca acgcgcggcc tcggccatgg
      601 cctgctccgg tgtgtagccg agcggcacct cgttgaagtt ggtcacccgc tcagccggtg
      661 gctgctccgg catcggttgc cgcggttgct tggacggatg gacttcagac atcagtgtgc
      721 ctccacgggg ccaaagcccc agatccctca ggcgtcgagg acgcccgcga tgcggcagtg
      781 ctcgtagttc tccgcgatct ggcgctcctc ggcccgatac gcgccttggc gcttcatcag
      841 cagatcgaag ttgaccaggt ggccgtcgaa ctccgggccg tccacgcacg cgaacttcgt
      901 ctgcccgtcc acctccaccc ggcccccccc gcacatcccg gtaccatcca ccatgatcgg
      961 gttcaggctt accatggtgg gaatgccgta ctgccgcgtc agctcggaca cggcccgcat
     1021 catgggtgtt gggccgaccg ccaagacgaa ccggatatcg gtaccctcgg cgatcagcgc
     1081 tgccagagca tcggtgacga accccttgcg accgtagctc ccgtcatcgg tggcgacctc
     1141 gagacggtcg cacagcgatc cgagctcctt ctcgaggatc accagcccgc gggtccgcgc
     1201 gccgatgatg ccgatgagcc ggttgccggc gccacgcag
//
LOCUS       DAOSPL010001530         1238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_112480_length_1238_cov_2.942519, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001530 DAOSPL010000000
VERSION     DAOSPL010001530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112480_length_1238_cov_2.942519"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..>1238
                     /locus_tag="PLQ54_21180"
     CDS             200..>1238
                     /locus_tag="PLQ54_21180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014320985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOS95835.1"
                     /translation="MSDRPQGVDTIALHAGHTPDADTLSRAVPIYQTTSYLFRNTEHA
                     ANLFSLKETGFIYSRIMNPTVDVLEKRLAALHGGTASVTFASGQAAIFAVVATLSSAG
                     QNFVTGSNLYGGTSTLFSFPLKRFGIEARFVDSSDPANFERAIDANTRLLYVESVGNP
                     RCNVDDLQGIAEVAHRHDIPFVVDNTFTPPPIHNPFDFGADLVVYSLTKMIGGHGTSI
                     GGAVVEKGDFDWKASGRFPELTEPDPAYHGVSFWDSFGNHDQAAIRGVAFSLKARTGI
                     LRDVGGCLSPFNAFLFIQGIETLPLRARAHTENARKVAQFLESHPCVSWVNYPGLPSH
                     PDHARSQRYFPL"
BASE COUNT          221 a          458 c          334 g          225 t
ORIGIN      
        1 gccgcttcct gcgcggaccg agctggcgcc cgtgcccccc gcgtccggct cgacccacaa
       61 ccagcccaag tcccccgcct ggctgcgcca ggcaggcctc atcgccgcgc cgaggaacac
      121 gccctatcgc caccgatgct atacagaggg atagccgccg cgccttggcg gcacttcata
      181 cagggaagca gggagactca tgtctgaccg ccctcagggc gtcgatacca tcgccctcca
      241 cgctgggcac acgcccgatg ccgatacact gtctcgcgcc gtaccgatct accagaccac
      301 gagctacctg ttccgcaata ccgagcacgc cgcgaacctg ttctccctca aggaaacggg
      361 cttcatctat agccgcatca tgaaccccac ggtcgatgtg cttgagaagc gcctcgctgc
      421 cctccatggc gggacggcct ccgtcacctt cgcgtcgggg caggccgcca tcttcgcggt
      481 cgtggctacc ctttcgtcgg cgggacagaa cttcgtcacg ggcagcaatc tatatggagg
      541 cacctccacg ctcttctcat tccctctcaa gcgctttggg atcgaggcac gattcgtcga
      601 ctcgtctgac ccggccaact tcgagcgggc catcgatgcg aacacacggc tgctgtacgt
      661 cgagtcggtc ggcaatcccc gatgcaacgt cgatgacctg caaggcattg ccgaggttgc
      721 acaccgccac gacatcccgt tcgtcgtgga caacaccttc acgccgcccc cgatccacaa
      781 cccgttcgat ttcggcgccg atctggtcgt ctactcactg accaagatga tcggcggaca
      841 cggcacatct atcggcggag ccgtggttga gaagggcgat ttcgactgga aggcctccgg
      901 ccggttcccc gagctgacgg agcctgatcc tgcctaccac ggggtcagtt tctgggacag
      961 cttcggtaac cacgaccagg ccgccatccg cggcgttgcc ttctcactca aagcccgaac
     1021 cgggatcctc cgcgatgtgg gtggctgcct ctcgcccttc aacgccttcc tcttcatcca
     1081 aggtatcgag accctccctc tgcgcgctcg agcccacacc gagaacgcga ggaaggtggc
     1141 tcagttcctt gagagccacc cctgcgtctc gtgggtcaac tacccgggcc tcccgagcca
     1201 tcccgaccat gctcgaagcc aacgctactt ccccttgg
//
LOCUS       DAOSPL010001531         1237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_112653_length_1237_cov_2.269882, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001531 DAOSPL010000000
VERSION     DAOSPL010001531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112653_length_1237_cov_2.269882"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1237
                     /locus_tag="PLQ54_21185"
     CDS             <1..>1237
                     /locus_tag="PLQ54_21185"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOS95836.1"
                     /translation="GDEVEVDVWSGALSRRVWHAAVDSEGELGELTGVALNADGEPLA
                     GAVVWVDLLPLESSWAGSTTGRRTETDASGRFTLAGVRPGYHTVAVLAGGATVALHPL
                     YVGLPGEREPVVVRAVRPTGKVVGRVIEHGTEQGLDGVTVVAIEQRYLRQQSADIARR
                     VPRRPRAGVRRAVSTGDSMPESDLQAVLAEASTRPDGSYEIAGLLPGAYAVAAIGAES
                     GRDGVGPVFVTGDSTTRAPDLWLPRREGIPVRVRLVTEAGEPLGQQMVELTARGGGAE
                     GSIVAQGPVDETGLLTLRATSPGWYGFSLRCSDGSEAQCWGVPVTAQGPTVEPTLTAR
                     RPARGRATVRIITPDGVPVSQCVWVFPVAQPGGESGDAADFGRTTPTDAEGVCVFEGL
                     EPGPYMFVAQTPHTLETDG"
BASE COUNT          179 a          398 c          465 g          195 t
ORIGIN      
        1 ccggcgatga ggtggaagtc gatgtctggt ccggtgcttt gagccgtcgg gtctggcacg
       61 cggccgttga tagcgaaggc gagctcggcg agctgaccgg ggtggcactg aacgccgatg
      121 gcgagcccct ggcgggtgcc gtcgtgtggg tggacctgct ccctctggag tcctcgtggg
      181 ctggttccac gactggtcgc cgcaccgaga cggatgcatc ggggcgcttc accctggccg
      241 gagtgcggcc tggctatcat accgttgccg tgctggctgg cggcgccacc gtggcgctcc
      301 atccgctcta cgttgggttg ccgggcgaac gcgagccggt ggtggtccgt gcggtgcggc
      361 ccaccggcaa ggtggtgggg cgggtcatcg agcatgggac cgaacaggga ctcgatggag
      421 tcaccgtcgt ggccatcgag cagcggtacc tgcgccagca gagtgccgac attgcccgcc
      481 gcgtcccgcg ccgcccgagg gccggtgtgc gtcgggccgt atccaccggt gactccatgc
      541 ctgagagcga cctgcaggcc gtgctggcgg aggcgagtac acgcccagat gggtcgtacg
      601 agatcgccgg tctcctgccc ggcgcctacg ctgtggcggc catcggcgcc gagagcggcc
      661 gagacggagt ggggccggtc tttgtcaccg gcgactcgac gacaagagcc ccggacctat
      721 ggctgcctcg ccgcgaaggc attccagttc gggtccggct cgttacggag gccggggaac
      781 cccttggcca acagatggtc gaactgacgg cacggggcgg cggcgccgaa ggcagcatcg
      841 tggcacaggg gccagtcgat gagacaggac tgctcactct ccgcgcaacg agccctggct
      901 ggtatggctt cagcctccgg tgcagcgatg gctcagaggc ccagtgctgg ggtgtgcctg
      961 tgacggcgca ggggccaacc gttgagccca cgctcaccgc tcgccggccc gcgcgcggcc
     1021 gggcgacggt gcggatcatc acccccgacg gggtgccggt ctcgcagtgc gtatgggtgt
     1081 tcccggtggc acagcccgga ggcgagagcg gtgacgctgc cgacttcggt cgcacgacac
     1141 ctaccgacgc cgaaggggtg tgtgtcttcg agggactgga gcccggccca tacatgttcg
     1201 tggcccagac gccgcacacg ctcgagacag acggata
//
LOCUS       DAOSPL010001532         1236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_112769_length_1236_cov_2.667231, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001532 DAOSPL010000000
VERSION     DAOSPL010001532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112769_length_1236_cov_2.667231"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLQ54_21190"
     CDS             complement(<1..191)
                     /locus_tag="PLQ54_21190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOS95837.1"
                     /translation="MAQAFPEVQGRVPYEGPETKNPLAFRHYNADEVIEGKTMRDHLR
                     FSVAYWHTFRGTGRDPFGDA"
     gene            complement(242..913)
                     /locus_tag="PLQ54_21195"
     CDS             complement(242..913)
                     /locus_tag="PLQ54_21195"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOS95838.1"
                     /translation="MSTRGPKLAASMMCADALAMEPDLRALERAGIEYLHIDMMDGHF
                     VPNIAMGVDLVRQIQSGTSIPLDVHLMVTNPEFWVPKVLEDLSPAIVAFHHEAAPHGV
                     RLAQTIRARGVLAGVAINPGTAVSALDALLPEVDLVLVMTVNPGYAGQRIVKSAVAKI
                     GELSERRARLGLPLLIQVDGNVSFESAPEMVALGADILVGGSSLFRKDVGIEEAARQL
                     RVRSA"
     gene            complement(938..>1236)
                     /locus_tag="PLQ54_21200"
     CDS             complement(938..>1236)
                     /locus_tag="PLQ54_21200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOS95839.1"
                     /translation="QEQAVRMLNFRGRLNLFGGLPPGSRTIAFDSNFVHYREAIVTGS
                     HGSVPRQHALALKLLASGAIDGRRYFTHAFALDQAVEAFAVAASKEALKVSIRP"
BASE COUNT          229 a          413 c          389 g          205 t
ORIGIN      
        1 gcatcgccga acggatcgcg ccccgtcccg cggaacgtgt gccagtaggc caccgagaac
       61 cgcaggtggt ccctcatggt cttgccttcg atgacctcgt cggcattgta gtgccggaag
      121 gcgagcgggt tcttggtctc ggggccctca tagggcactc ggccctgcac ctcggggaat
      181 gcctgcgcca tcgcggaaca ccatcgctct ccttggggat tggggacagc cactgaaatc
      241 cttaggcact gcgtacccga agctgccggg cggcctcttc gatcccgaca tccttgcgga
      301 acagactcga accacccacc agaatgtccg cgcccagggc caccatctcg ggagcgctct
      361 cgaagcttac attgccatcg acctgaatga gaaggggcag cccgagccgt gccctccgct
      421 cactgagctc accgatcttg gccaccgcgc tcttcacaat gcgctggccc gcataccccg
      481 gattcacggt catcaccaac accaggtcga cctcgggcag cagggcgtcg agggcactca
      541 ctgccgtgcc ggggttgatg gcgacaccgg ccagcacgcc ccgcgcccgg atggtctggg
      601 caaggcgcac gccatgcggc gccgcctcat ggtgaaaagc aacgatggcc ggagagagat
      661 cctcgagcac cttgggcacc cagaactccg gattcgtcac catcaggtgc acgtcgagcg
      721 gaatcgacgt gcccgactgg atctggcgta ccagatcgac tcccatggcg atgttgggca
      781 cgaagtgtcc atccatcatg tcgatatgga ggtactcaat gcccgcgcgc tccaatgcac
      841 gtaggtccgg ctccatggcg agagcgtcgg cgcacatcat cgacgcggcg agtttgggac
      901 cccgggtact catacgttct cgcacaccct ccagcggtca cgggcggatg gagaccttga
      961 gggcctcctt ggacgcggcc acggcgaatg cctcgaccgc ctgatccaga gcgaaggcat
     1021 gggtgaagta ccgccggcca tcaatggcac ccgaggcgag cagcttcaac gccagcgcgt
     1081 gctgccgggg cacactgccg tgtgaccccg tgactatggc ctcgcggtag tggacgaagt
     1141 tgctgtcgaa ggcaatggtg cgcgagccgg ggggcagccc gccgaacagg ttgagccgac
     1201 cccggaagtt gagcatccgt acggcctgtt cttggg
//
LOCUS       DAOSPL010001533         1234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_113075_length_1234_cov_2.282443, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001533 DAOSPL010000000
VERSION     DAOSPL010001533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113075_length_1234_cov_2.282443"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PLQ54_21205"
     CDS             complement(<1..630)
                     /locus_tag="PLQ54_21205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOS95840.1"
                     /translation="MARWDRQHWDGDPSGMSRADRASGTFEAYIPDPLMGRIVPLATD
                     TARLLAQADRAVAGMGQGDRTAHLGDLAGLLLRTEAAASSWIEGIPPRARQVAFAALA
                     QEEDDLKRPGEAAQLVANNIHIMRTAVAEMAGVGALTVEQLVGLQEDLVLDHRAHGLR
                     RTQNWIGGSPGHPLTAAFVPPPPEHVQSLLNDLVTYLNDALVLAPSMLLD"
BASE COUNT          233 a          431 c          359 g          211 t
ORIGIN      
        1 gtcgagcaac atggacggcg cgagcacgag ggcgtcgttg aggtaggtca ccagatcgtt
       61 gagaagcgac tggacgtgct ctgggggtgg aggaacgaaa gctgccgtca gtgggtggcc
      121 aggagatccg ccgatccagt tctgggtgcg ccgaagccca tgcgctcggt ggtcgagcac
      181 caagtcctcc tgcaggccga ccaactgctc cacggtcaac gcaccaacac ctgccatttc
      241 ggccacggcc gttcgcatga tgtggatgtt gttcgccacg agttgagcgg cttcaccagg
      301 tctcttgagg tcatcctcct cttgcgcgag cgccgcgaag gccacctgtc gagcgcgcgg
      361 aggaatcccc tcgatccacg aactcgccgc tgcttccgtc ctcaacaaca ggccggcaag
      421 gtcgccgaga tgggcggtcc ggtcaccctg acccatcccg gcgacagctc ggtccgcttg
      481 ggccagcagc cgggcggtgt cagtggcgag cggcacgata cgacccatga gcggatcagg
      541 gatgtacgcc tcgaaggtgc cagaagcgcg gtccgccctg gacatgcccg acggatcacc
      601 gtcccagtgc tgccgatccc accgcgccat cgcgagctcc ttcgcctcaa gtcgaaaggt
      661 aacccaacac accggccctg tctagcaata gattactctg cgtcagattc ctattcacat
      721 gttcagtagt tgctcacgag gttaccccgt cttctgagac tggacatccc cgctgacgcg
      781 gggcgcacgt ccagatccct gtccacatgg gctcatctcc gcaccgcgga gccaacgccc
      841 tccaaggatg catcggcgct gtgtcgttgg gccagagccg ctcggcacat cgacccggac
      901 tcccgtctca ggcggaggtt ctcacaagtg ccgatcctgc gccgggatca cgaccaacca
      961 tcaggtctag caaggccccg ttgtcatcga gatcaacacc tcggcgcaca cggccacctc
     1021 cgagcgggcg cacgcggtat cgagacgggt tctcgtccct ctcgtcggca ctatcgagtt
     1081 ccatcctcca agtttgcccg caagcgtgtc ctgccacgcc cagagacaat tccccgccga
     1141 aagtgctggc gacgagccgc cccacgaaac ccgagggcgc acggcataca ccgtttgaca
     1201 caatcgcccc atgcctcgcc ccatcgtcca gagc
//
LOCUS       DAOSPL010001534         1233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_113191_length_1233_cov_2.485569, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001534 DAOSPL010000000
VERSION     DAOSPL010001534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113191_length_1233_cov_2.485569"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PLQ54_21210"
     CDS             complement(<1..386)
                     /locus_tag="PLQ54_21210"
                     /inference="COORDINATES: protein motif:HMM:NF018621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1284 domain-containing protein"
                     /protein_id="HOS95841.1"
                     /translation="MRARPHHLIDIITQYGAGQPFAPCDYGHAVHTVAARVIGDPQTL
                     IEFVVGADDICDPCKHLVDGRCDDGVPALGPTASKQEYNDALDERLMQFLGITEGQTM
                     TFREYISVLRDHWDGLVEQCAHPGESP"
     gene            630..>1233
                     /locus_tag="PLQ54_21215"
     CDS             630..>1233
                     /locus_tag="PLQ54_21215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95842.1"
                     /translation="MAGTQKTGLTGAGKVVVTLIVLGLIGAGGYLLRDKLLPKGASGG
                     GEVDLDAYKSQQAGQQAEKADLTGITTVTEYSFVPSEKLPPVKGTSNYKWNDADPVVV
                     FPINVWIGWLPIVAANHGFAANTESIFYKDYGFKVQLKLIDDPVKARDAFAAGDSHIL
                     WGTLDMMVLFAPELMKDSRTAPRIFQQIDWSSGGDGIVVRE"
BASE COUNT          215 a          395 c          422 g          201 t
ORIGIN      
        1 ggcgactcac cgggatgggc acactgctcg accaggccgt cccagtggtc gcgcaggacg
       61 gaaatgtact ccctgaacgt catggtctgg ccctcggtga tcccgaggaa ctgcatgagc
      121 cgctcgtcga gagcgtcgtt gtactcctgt ttcgaggcgg tgggcccaag agcgggcacc
      181 ccgtcatcgc agcggccgtc gacgaggtgc ttgcaggggt cacagatgtc gtcggctcca
      241 acaacgaact cgatgagtgt ttggggatcg ccaatcaccc gggccgcgac ggtgtgaacc
      301 gcgtggccgt agtcgcaggg cgcgaagggc tgacccgcgc cgtactgagt gatgatatcg
      361 atcaggtggt gcggtcgcgc gcgcaaggga ggccccctcc tcggtttccg tgccgccgtc
      421 tgtgccgccc gcatggcttc cccggccgcc cgccgggccc ctgcatggac cgcgtgggca
      481 aacgctggcg caggagttgc gccgcgccgc gcgaaggtca tgcagtcggc ggtgtggccc
      541 gagcgccact cggggctatc gaggccgtct cttcaggagt ggtactctcg agccgacgac
      601 gcgtggatcg gctgttggag gcatcgacca tggcaggcac acagaagacg ggcctcaccg
      661 gagcggggaa ggttgtcgtc accctgatcg tcctcggtct gatcggggcc ggcggctacc
      721 tcctgcgcga caagcttctg ccgaaaggcg cgtccggtgg cggcgaagtg gacctggacg
      781 cctacaagtc gcagcaggcg gggcagcagg cagagaaggc ggacctcacc ggcatcacga
      841 cagtcaccga gtacagcttc gtgccctcgg agaagctccc gcccgtcaaa ggcaccagca
      901 actacaagtg gaatgacgcg gaccccgtgg tcgtcttccc gatcaacgtc tggatcggtt
      961 ggctgcccat cgtcgcggcc aaccacgggt tcgccgccaa cacggagagc atcttctaca
     1021 aggactacgg gttcaaggtc cagctcaagc tcatcgatga cccggtgaag gcacgagacg
     1081 ccttcgcggc cggtgacagc cacattctgt gggggacgct ggacatgatg gtcctgttcg
     1141 cccccgagct gatgaaggac agccgcacgg cgcctcgcat cttccagcag atcgactggt
     1201 ccagcggcgg cgacggcatc gtggtccgtg agg
//
LOCUS       DAOSPL010001535         1227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_114021_length_1227_cov_3.246587, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001535 DAOSPL010000000
VERSION     DAOSPL010001535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114021_length_1227_cov_3.246587"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..>1227)
                     /locus_tag="PLQ54_21220"
     CDS             complement(119..>1227)
                     /locus_tag="PLQ54_21220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95843.1"
                     /translation="PAATIHGLDKPERWPGNALPDASAWPVTYHEADQYPLWLMAEWR
                     GMKWSGFDFVLVDTWYSLCFDQEAKAGPPFEALAKAWAELEKLGEEPLPMAMFLETPF
                     AWHGTKDGDCTEASADGIIELWEPTRGFLRQFYGEEGYEPRLPLRALARVNVNGEARP
                     ILQFWFPTWVDTGITKWNEWTFAELRRLCREAFGVEPFIGVNQHVHGREFVGGWNGKQ
                     PGGGTLDISSAAGVVDYDVAWWGGMAGPQIYPNAIVLGPGHWCPRQTGDKPVTLHWSA
                     EYAPDSSRYEHCWRQVLSNPDSFGRKLLLVESWNNSDEGCAIAYSEPKDFRTEAGDLI
                     DRWGDTPEYYMTLTRELAPYWREGRCPERFVPKP"
BASE COUNT          192 a          402 c          418 g          215 t
ORIGIN      
        1 gtcgcatctg tacttcacga accaggcggg cgacagggtc tggcgactgc cttacacgat
       61 ggccgaggac tttgccgagc cggagagggt gcggtgagag cgatcccggg aaagcctgct
      121 acggcttggg gacgaagcgc tccgggcagc ggccttccct ccagtagggc gccagttccc
      181 tcgtcagcgt catgtagtac tcgggcgtgt cgccccagcg gtcgatcagg tcgccggctt
      241 cggtgcggaa gtctttgggt tcgctgtagg cgatggcaca gccctcatcc gagttattcc
      301 agctctcgac gaggaggagc ttccggccga agctgtccgg gttcgagagt acctggcgcc
      361 agcagtgctc gtagcgcgag ctgtcggggg cgtactcggc gctccaatgc agcgtgaccg
      421 gcttgtcgcc ggtctgacgc gggcaccagt gccccgggcc caggacgatg gcgttcgggt
      481 agatctgcgg tccggccatt ccgccccacc aggcgacgtc gtagtcgacc acgcccgcgg
      541 ccgatgagat gtcgagcgtt ccgccgccag gctgcttccc gttccatccg ccgacgaact
      601 cgcggccgtg tacgtgctgg ttcacgccga tgaagggctc caccccgaag gcctcccggc
      661 agagccggcg cagctcggcg aaggtccact cgttccattt ggtgattccc gtatcgaccc
      721 acgtggggaa ccagaactgg aggatgggcc gggcctcccc gttcacgttc acccgtgcca
      781 gggcgcgcag agggagacga ggctcgtagc cctcctcacc gtagaactgc ctgaggaagc
      841 cacgggtggg ctcccagagc tcgatgatgc catcagcgga tgcctcggtg cagtcgccat
      901 ccttggtccc gtgccatgcg aagggcgtct cgaggaacat ggccataggc agtggctcct
      961 cgccgagctt ctcaagctcc gcccacgcct tcgccagtgc ctcgaagggt ggtccggcct
     1021 tcgcctcctg gtcgaagcac agcgagtacc aggtgtcgac gaggacgaag tcgaagccgc
     1081 tccacttcat gccgcgccac tcggccatga gccacagggg gtactggtcc gcctcgtggt
     1141 aggtcactgg ccaggccgag gcgtcgggca gcgcgttacc gggccatcgc tcgggcttgt
     1201 cgaggccgtg gatcgtggcg gcaggat
//
LOCUS       DAOSPL010001536         1219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_115082_length_1219_cov_2.664089, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001536 DAOSPL010000000
VERSION     DAOSPL010001536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115082_length_1219_cov_2.664089"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLQ54_21225"
     CDS             complement(<1..287)
                     /locus_tag="PLQ54_21225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95844.1"
                     /translation="MRCRQARMLIHETLDERTGAWPPGLESHLRECPSCQRLASRLVA
                     LSALARQASAEDPSDAHLRDIARRAAAGARWAPQALQPRPLRVSLAGALSWS"
     gene            complement(343..906)
                     /locus_tag="PLQ54_21230"
     CDS             complement(343..906)
                     /locus_tag="PLQ54_21230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOS95845.1"
                     /translation="MGRAQVAMVQTPQSLVSRFAEGDTAAFDEVVRLYGRDVYRVARR
                     MTDCADDADDVVQETFIRAFQRLGEVDGDASVKRWLLTITINLCIDRHRRRRLPVSEL
                     SFDPPAERSAADHELLNRELGQHLHAALSKLSGQQRACFVLHEIEGLKVAEIAQIVGC
                     SDGAVKCYLHRARDRLQKQLAPYLLAP"
BASE COUNT          206 a          433 c          395 g          185 t
ORIGIN      
        1 gaccatgaca gggccccggc caacgagacc cgcagaggcc gcggctgcag cgcctggggt
       61 gcccatcggg ccccagccgc tgcccggcga gcgatgtcgc gcagatgggc gtcgctcggg
      121 tcttccgccg aagcttgccg cgcgagcgcc gacagcgcca ccaggcgcga cgcgagccgc
      181 tgacaggagg ggcattccct caggtgggac tcgagaccgg gaggccacgc tcccgttcgc
      241 tcgtccaacg tctcgtggat gagcatcctt gcttgacggc acctcatcgc cgtatcaccc
      301 ctcgcgtcgc caccgcgtcg tcagcgtggc gcttccgaca cctcatggcg ccagtaggta
      361 tggggccaac tgcttctgca accggtcccg ggcacgatgg agatagcact tcaccgcccc
      421 gtcggagcac ccgacgatct gggcgatctc cgcaaccttc aacccctcga tctcgtgcag
      481 gacgaagcac gcccgctgct ggcctgagag cttcgagagg gccgcatgca ggtgttggcc
      541 gagctctcgg ttgaggagtt catggtccgc cgcagagcgt tctgccggag ggtcgaaaga
      601 gagctcactg accggaagcc ggcggcggcg gtggcggtca atacacaggt tgatggtgat
      661 cgtcaggagc cagcgtttca cactggcgtc gccgtccacc tcacccaacc gctggaatgc
      721 ccggatgaag gtctcctgca ccacgtcgtc ggcatcatcg gcgcaatcag tcatccggcg
      781 cgccacacgg tacacgtcgc gaccatacag ccgcacgacc tcgtcgaacg cggcggtgtc
      841 gccctcagcg aaacgcgaga ccagtgactg tggcgtctgc accatggcca cctgggcacg
      901 tcccatgaac tcgtcgcctc ccgccgccaa tcggcggttc catccacagg tctagaacgc
      961 tcgggagcgc ggaaggcttg cagggggcat cgatatcatt agcgctttca ttcgccgcgg
     1021 gttggccgcg gccaatgtgg ggcgcccggg ccaccgccca ccatggcagc ggttcagact
     1081 ccggcgagcc ggtcgacact cagggacgac tgcccgtcgg gcacagcgac aggctcaaca
     1141 ccaagaggcc gcctgctacg gctcgagcct acccctgccc gccctcgctc gccccgccgc
     1201 gataggtggg aggcggggg
//
LOCUS       DAOSPL010001537         1216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_115470_length_1216_cov_4.626184, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001537 DAOSPL010000000
VERSION     DAOSPL010001537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115470_length_1216_cov_4.626184"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="PLQ54_21235"
     CDS             complement(<1..1085)
                     /locus_tag="PLQ54_21235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95846.1"
                     /translation="MVFTHATVRKVDSFDDGWWHLRNGQVIVEERRIPTVDEYSYTKR
                     GQPRVPHEWLSEATLYLSMKLAGFPGIMVWRGVIAASTTILAAWFLLLRGMRSAPLAV
                     FFLMLGAAISVPEWLERPKIAAPMFFIAALLLLELCERRRRWACYGLPVLMLLWANSH
                     ASFIAGAGLIGLRLACAWTEERAGLAEKAFCGYLMPWAALALLAPVVSPGGLATIQNP
                     LTYLSGSTSWGLEEFQEWASPNFHKPHGLMFEAGLMVVLISLALSPKRPRLYDLLVVL
                     VTTHLPLQSVRHYAIFGAGAMPVAARYADAAAARLAEWGGVANLRASLDRLRARAGRM
                     GRRVWGGVSVALTAALVAVAIPKDGSF"
BASE COUNT          247 a          433 c          365 g          171 t
ORIGIN      
        1 tcaaaggacc cgtcctttgg gatggccacc gccaccaggg cggccgtcag ggccacagag
       61 acgccccccc acacccgccg tcccattcgt cccgctcggg cgcggagtcg atccagcgag
      121 gcccgcagat tggccacccc accccactcg gccaaccgcg ctgctgcggc atcggcatat
      181 ctggccgcca cgggcatcgc acccgctccg aagatggcat agtgccgaac cgattgcagg
      241 ggcagatgcg tcgtcaccaa cacgacgagc agatcgtaca gccgcggtct cttgggcgat
      301 agggccaggc tgatcaacac aaccatgagg ccggcctcga acatcaaccc gtgcggcttg
      361 tggaagttgg gcgacgccca ctcctggaac tcctcgagcc cccacgaggt cgacccgctc
      421 agataggtga gcggattctg aatcgtcgcg agcccccctg gcgagaccac cggtgccagc
      481 agcgccagcg ccgcccaagg catcaggtaa ccgcagaaag ccttctcggc caaaccagcc
      541 cgctcttccg tccacgcgca cgccagccga agtccaatca agcccgcacc tgcgatgaaa
      601 ctcgcgtggc tgttcgccca gagcagcatc aggaccggca gaccatagca ggcccatctc
      661 cggcggcgtt cgcacagctc gagcagcagc agggcggcga tgaaaaacat gggcgccgcg
      721 atcttgggtc gctcaagcca ctcgggtacg gagatggccg caccgagcat caggaagaac
      781 accgctaacg gcgccgagcg catcccgcgc aggagtagga accacgcggc caggatcgtg
      841 gtcgacgccg caatgacacc gcgccagacc atgatgccgg ggaacccggc gagcttcatc
      901 gagaggtaga gagtcgcttc cgacagccat tcgtgaggga cccggggctg gcctctcttg
      961 gtgtacgagt actcatcgac agtcggtatc cggcgctcct ccacgatgac ctgcccgttc
     1021 cgaaggtgcc accagccgtc atcaaaggag tccaccttgc gtactgtcgc gtgtgtaaat
     1081 accaaggcgg ccaccggcgc gacccacacc cacgccggca caggcagctt cgcaggagtg
     1141 tcgccttgtg cggattcacc caagtccagt gccccccaag gtcatggccc acacggggca
     1201 taggttcggt cgggtc
//
LOCUS       DAOSPL010001538         1214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_115805_length_1214_cov_2.867127, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001538 DAOSPL010000000
VERSION     DAOSPL010001538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115805_length_1214_cov_2.867127"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PLQ54_21240"
     CDS             complement(<1..671)
                     /locus_tag="PLQ54_21240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95847.1"
                     /translation="MSSYPNDTGPTGPERAKRAPVAPAGSRFRVALAAYYATVIAGTL
                     SMMVATRMADGLLTSVALGLLMLSRRFSSTGSNLVQLGTSQTLLRYVGMHADEPATKR
                     RYVAFAAWAWVALLAICLPITIAYQELLAGWWFPDTSAGGAMAVWSVLLMFSIMAIYI
                     AHSLLLAEQRMVWAGLVAGMENGGFMLGLLLVLRSESTPLNLVAYQAVGATVLSAAVV
                     LWYCLG"
     gene            complement(664..>1214)
                     /locus_tag="PLQ54_21245"
     CDS             complement(664..>1214)
                     /locus_tag="PLQ54_21245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95848.1"
                     /translation="TLGPCMISVRVNVGAVGGHWSNTAEEGGRFLGEGVHFFDLCNWF
                     VGEEPEAVCAMFCGDNEVTNPNGEVLVRYPDGSTAHITYTALGHAGMGKEYIEVFRNG
                     RAAVCDDFRTLRCFGGRAAAGRGARGDKGQLEELKEFAAAVRGRPHPVAGADARAGLV
                     ATWLAVSSYQSARTRAWIIRDV"
BASE COUNT          205 a          417 c          414 g          178 t
ORIGIN      
        1 ccaaggcagt accagaggac tacggcggcg ctgaggaccg tagcgccgac ggcctggtag
       61 gcgaccaggt tgagcggcgt cgattcgctc ctgagcacca gcagcagccc gagcatgaag
      121 ccgccgttct ccatccccgc cacgagcccg gcccagacca tgcgctgttc agccagcaga
      181 agcgagtgcg cgatgtagat ggccatgatg ctgaacatca gcagcacgga ccacacggcc
      241 atggcgccgc ctgccgacgt gtccgggaac caccagccgg cgaggagctc ctggtaggcg
      301 atggtgatgg ggaggcagat ggcgagcagc gcgacccacg cccaggccgc gaaggcaaca
      361 taccgccgct tggtggcggg ctcgtccgcg tgcatcccca cgtagcggag tagcgtctgc
      421 gaggtcccga gctgcaccag gttggagccg gtgctgctga agcgtcggga tagcatcagc
      481 aggcctagcg ccaccgaggt cagtaggcca tccgccatcc gcgtagcgac catcatcgag
      541 agggtgcccg cgatcacggt ggcgtagtag gcggccaggg ccactcggaa cctcgatccc
      601 gcgggcgcga ccggtgcgcg cttggccctc tccgggccgg tgggtccggt gtcgttgggg
      661 tagctagaca tcacggatga tccaggctcg ggtccgagcg ctctggtagc tgctgaccgc
      721 tagccaggtg gccacgagcc ccgctcgagc gtcggcgcca gccaccgggt gcggccggcc
      781 gcgcaccgcg gcggcgaact ccttgagttc ctcgagctgc cccttatcgc cgcgggctcc
      841 gcgaccggca gcggcccgcc ccccgaagca acggagcgtg cggaagtcat cgcacacggc
      901 cgcgcgcccg ttgcggaaca cctcgatgta ctccttgccc ataccggcgt gcccgagtgc
      961 cgtgtaggtg atgtgtgcgg tcgatccatc cgggtagcgg accagcacct cgccattggg
     1021 gttcgtcact tcgttgtcgc cgcagaacat ggcacagacg gcctcgggct cttcgccgac
     1081 gaaccagttg cagaggtcga agaagtggac accctcgccc aggaatcggc cgccttcctc
     1141 agcggtgttg ctccagtggc cgccgacggc acccacgttc acgcgcaccg agatcatgca
     1201 tgggcccagg gtgc
//
LOCUS       DAOSPL010001539         1211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_116277_length_1211_cov_3.359862, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001539 DAOSPL010000000
VERSION     DAOSPL010001539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116277_length_1211_cov_3.359862"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..748)
                     /locus_tag="PLQ54_21250"
     CDS             complement(164..748)
                     /locus_tag="PLQ54_21250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95849.1"
                     /translation="MEAHGGTTPGPAEVPEIAHQSAAVLVGAGRAAHTPEVTARLVAL
                     VDDLGLDTIAELWAGRPARSLPGVLWRLYAVREWVRRSPEQASREYAAGIRFTDVAHA
                     VAGIPEPPSPTELARTVDQILSGVFEGDFAIALERAAAFCHVLAAGRAELAHDADATD
                     PGRAAELTERAAGIQTTARDLVAAAGLWRSGNLD"
     gene            951..1026
                     /locus_tag="PLQ54_21255"
     tRNA            951..1026
                     /locus_tag="PLQ54_21255"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:984..986,aa:Lys,seq:ttt)
BASE COUNT          184 a          408 c          424 g          195 t
ORIGIN      
        1 cgcgtggccg ctcgtagcgg ctgattttgg gacccggggc tgccgcggtc cgacatccac
       61 cagcgaccct aacgcacaga ggcgcctggt ccttccccga ttcgatgtat tccgcactca
      121 gatcctgtgt cccggataca tcgaaccagc cggaggagcg gactcagtcg aggttgccgg
      181 agcgccagag gccagccgcg gcgacgaggt cacgagccgt tgtctggatg ccggcggccc
      241 gctcggtgag ttcggcggct cgccccgggt cagtggcgtc ggcatcgtgg gcgagttcag
      301 cacgtccggc cgcgaggaca tggcagaagg ctgcggcccg ctccaaggca atggcgaagt
      361 ccccctcgaa gacgcccgac aggatctggt cgaccgtccg cgccaactcc gtcggggacg
      421 gcggctccgg gatcccggcg accgcatggg cgacatcggt gaaccggatg cccgccgcat
      481 actcccggct ggcttgctcg ggcgagcgac gcacccactc gcgcacggca tacaaccgcc
      541 agagcacccc gggaagcgac cgggcagggc ggcctgccca gagctcggca atggtgtcca
      601 gaccgaggtc atcgacgagg gcgaccaagc gagcggtgac ctcgggcgtg tgggcggcac
      661 ggcctgctcc cacgaggacc gcggcactct ggtgcgcgat ctcggggacc tcggcgggcc
      721 ctggggtggt gccaccatgc gcttccatcg cctccggacc gaagtgcacg ggacggcgcg
      781 ggaggtcgcg gccgtggggc gtccccgagg tgttcaccgg acaagtgtgc actctcggcc
      841 cgctgcggct cagcctccag acggcgtatg ccgtgtgccg gcgggttctc gcggcgcctc
      901 cggaggcggg taatctggcc accggccgca accctgctcg ggtgcgttca gggcctctag
      961 ctcaatggtt agagctgcgg acttttaatc cgtaggttgt gggttcgagc cccacggggc
     1021 ccaccatctg tgcaggtcag agggtgtttc tccctatcga acaccgacct ccgaccaccc
     1081 atgtacccgc tttggtaccg aactgcgtca ctggagcagg gtgccgatct tgaactcggg
     1141 gagtttcgct aaggcgtccg gggtgctcgc gtgccggttg ttgaagccca tgctgccgtc
     1201 ggtgccacag a
//
LOCUS       DAOSPL010001540         1205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_117130_length_1205_cov_3.462609, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001540 DAOSPL010000000
VERSION     DAOSPL010001540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117130_length_1205_cov_3.462609"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PLQ54_21260"
     CDS             complement(<1..845)
                     /locus_tag="PLQ54_21260"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOS95850.1"
                     /translation="MGGCRLCAKVLLAMAALSGGGVSAAPVPVTLERPAAGWQLMREG
                     APYPVRGVAGEVHLGLAARIGANSIRTWGVSDSLPALLDEAAAHGLTVAVGLWLGHER
                     HGFRYDEPQQVVGQLEACREAILRFRDHPAVLLWGIGNEMEAEGRNVAIWQHVNEIAA
                     MAKELDPNHPTMTVISDCSEVKVRLLHEHCPSIDIVGMNSYGGAPTLPERYRAAGGTK
                     PFIVTEYGPLGRWESPKTPWGAPIEATSTAKAEQYRRALEAQQASPELCLGSYAFLWG
                     WKQEAT"
     gene            complement(848..>1205)
                     /locus_tag="PLQ54_21265"
     CDS             complement(848..>1205)
                     /locus_tag="PLQ54_21265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95851.1"
                     /translation="DMVELGVNSRIAVYGRGPLGVLGLYAGVMDRRIQHVVLDQPPVS
                     HRDHSVILMDILRFADMPQCAALLCPMRLTFLGAIPGEFEFTRGIYELCGHADRFGAA
                     SDLVEALGPSPGAREE"
BASE COUNT          194 a          428 c          402 g          181 t
ORIGIN      
        1 gtcgcctctt gcttccagcc ccacaggaag gcatacgacc ccaggcacag ctccgggctg
       61 gcctgttgtg cctcgagtgc ccgccgatac tgctcggcct tcgccgtgct cgttgcctcg
      121 atgggcgctc cccagggcgt cttgggcgac tcccaccggc cgagggggcc gtattcggtg
      181 acgatgaatg gcttggttcc gccagcagcg cggtaccgct ccggcagcgt cggcgcgccc
      241 ccgtaggagt tcatgccaac gatatcgatg ctcgggcagt gctcatgcag aaggcggact
      301 ttcacctcgg agcaatcact gatgacggtc atggtcgggt ggttcgggtc cagctccttc
      361 gccatggccg cgatctcgtt gacatgctgc cagatggcaa cgttgcgccc ctcggcctcc
      421 atctcgttgc cgatacccca caggagcact gccggatgat cgcggaagcg cagaatcgcc
      481 tcccggcagg cctcgagctg gccgacgacc tgctggggct catcgtagcg gaagccgtgg
      541 cgctcgtggc caagccacag gccaaccgca accgtaaggc cgtgcgccgc cgcctcgtcc
      601 agcagtgcgg gaagcgagtc actgaccccc caggtgcgga tcgagttggc gccgatgcgg
      661 gcggccaggc cgaggtggac ctcgcctgcc acgccccgga ctgggtaggg cgctccctcg
      721 cgcatgagct gccaacccgc ggccggcctc tccagcgtca ccggcactgg cgcggcagaa
      781 acgccaccgc ccgagagcgc agccatggca aggagcacct tggcgcatag gcgacagccg
      841 cccatggcta ctcctcccgc gcacctggcg aggggccaag cgcctccacc aggtcggacg
      901 ccgcgccgaa tcggtcggcg tgaccgcaca gctcgtagat cccgcgggtg aactcgaact
      961 cgccggggat cgcgccgagg aacgtgagcc gcatcgggca gagcagcgcc gcgcactgtg
     1021 gcatgtcggc gaaccggagg atgtccatca ggatcaccga gtggtcgcga tggctgaccg
     1081 gcggttggtc gaggaccacg tgctggatcc gccggtccat gacgccggcg tacagcccga
     1141 gcaccccgag cgggccgcgg ccgtacacgg caatccggct attgacgccc agctcgacca
     1201 tgtcg
//
LOCUS       DAOSPL010001541         1203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_117492_length_1203_cov_1.932056, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001541 DAOSPL010000000
VERSION     DAOSPL010001541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117492_length_1203_cov_1.932056"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="PLQ54_21270"
     CDS             complement(<1..1052)
                     /locus_tag="PLQ54_21270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95852.1"
                     /translation="MGIRWALALSLVTCMAACGALWAADAEAESAVALAMPLLEQARH
                     LHASGDPAWRDRHERALSYLRLAAVEAPSPRVFRLLGEAELLAGREQTAAEAFRQYLE
                     LGGGDGGESEACTFVVEQALRSGAEAMAAQDNSRAAECFRLALSLDPQNALARMAVES
                     LGATVPDSFWVSLPSAGPLGEPVRVPRQRAAALCAQRATEAAARGDWPAVTTYLETMV
                     GAGSPSCVAASLARASAALTGLRSDVGSAVLQQARDHGRLAGFAGLRSAVATAGGRLY
                     RAALLMPDDLRAYGRLNRALPAVTEAVSAYARDRVLVALGVASVGLDSLAPAGLSDLV
                     LEASNGATVTPIASAP"
BASE COUNT          194 a          433 c          412 g          164 t
ORIGIN      
        1 cagggtgcgc tcgcgatggg agtgacggtc gcaccgttac tcgcctcgag taccaggtcc
       61 gagaggccgg ctggtgccag tgagtccaac ccaacgctgg ccacgccgag ggccacgagt
      121 acgcgatcgc gcgcgtaggc gctaaccgcc tccgtgacgg ccggcagcgc gcggttcagg
      181 cgcccgtagg ccctgaggtc gtccggcatg agtagcgctg cgcggtagag gcggccgccc
      241 gccgtggcaa ccgcggagcg gagcccagca aacccggcga ggcggccgtg gtcgcgcgcc
      301 tgctgcaaga cggccgatcc cacatcggac cgaaggccgg tcagtgctgc cgacgctcgg
      361 gccagcgagg cggcaacaca gctggggctc ccggcgccca ccatggtctc gagataggtg
      421 gtcacggccg gccaatcgcc gcgagccgcg gcttcagtcg cccgctgggc acacagggct
      481 gccgcgcgtt ggcgcgggac ccgcaccggc tcaccgagcg gcccggccga tggcagtgat
      541 acccagaagc tgtccggaac ggtggcgccc aggctctcga ctgccatccg tgccagcgcg
      601 ttctggggat ccagagacag ggcaagccga aagcactccg ctgctcggct gttgtcctgg
      661 gcggccatgg cctccgcccc gctgcgcaat gcctgctcga cgacgaacgt gcaggcctca
      721 gactccccgc catcgcctcc accgagctcg agatactggc ggaaagcctc ggccgccgtc
      781 tgttcgcgcc cggcgagtag ctcggcctcg cccagcagcc gaaacacgcg tggacttggg
      841 gcctcaacag cagcgagacg caagtacgat agcgcccgct cgtgcctatc ccgccaggca
      901 ggatcaccgg acgcgtgtag atgccgcgcc tgctcgagca gtggcatggc cagggccacc
      961 gcggactcgg cctcggcgtc cgcggcccaa agcgcgccgc aagcggccat gcatgtcaca
     1021 aggctcaggg cgagtgccca tcggatgccc atcgtgtccc atcccagctc gtcccgtgca
     1081 acagaggtcg cgtcgttgcc ggggcgtcag tagtcaggcc agccaggact gtggccattc
     1141 gccacacggc acgcggtctc cttgtcaact cgcacttctc tttaaccggg gacgggtgca
     1201 ggg
//
LOCUS       DAOSPL010001542         1202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_117566_length_1202_cov_3.496077, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001542 DAOSPL010000000
VERSION     DAOSPL010001542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117566_length_1202_cov_3.496077"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /gene="purM"
                     /locus_tag="PLQ54_21275"
     CDS             <1..672
                     /gene="purM"
                     /locus_tag="PLQ54_21275"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOS95853.1"
                     /translation="FAEGCRQAECALIGGELAEVAGLLGPDEYDLVGFAVAVVDRKRI
                     IDGSRVRQGDVLIGIASSGLHSNGYSLARKVVFEQAGLGLHDPMPGTGQSVIDAMLEP
                     TLIYTRTVRSLIDAADVRGLAHITGGGLIDNVPRILPTGLRADIDTGSWPTHAVFTFI
                     QQAGGIDDAEMYRVFNMGIGLVAVVREQEAPRAISAAESAGARAFLIGRVAADSGAGP
                     RVVLH"
     gene            705..>1202
                     /gene="purN"
                     /locus_tag="PLQ54_21280"
     CDS             705..>1202
                     /gene="purN"
                     /locus_tag="PLQ54_21280"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOS95854.1"
                     /translation="MPGEFRIGVLASGRGSNLQSILDACDDGRIDGRVVVVVSDVAEA
                     YALERARRRGIEAVYHDPGRFRTKLEPEREAALAELLLDREVQLVCMAGFMRVIHEPL
                     LNAFPGRIMNIHPSLLPAFPGLHVQQQALDYGVKIAGCTVHFANGGVDAGPIICQGAV
                     EVAEDD"
BASE COUNT          185 a          404 c          404 g          209 t
ORIGIN      
        1 tttgctgaag ggtgccggca ggccgagtgc gccctcatcg gcggggagct ggccgaggtg
       61 gctgggctcc tgggccccga cgaatacgac ctcgtaggct tcgccgtggc cgtcgtggac
      121 cggaagcgca tcatcgacgg ctcccgcgtg cgccagggcg acgtcctgat cggcatcgcc
      181 tccagcgggc tgcactcgaa tggctacagc ctggcgcgca aggtcgtctt cgagcaggcg
      241 ggccttggcc tgcacgaccc gatgccgggc acggggcagt cagtcatcga cgcaatgctc
      301 gaacccacgc tcatctacac ccgaaccgtc cgctcgctga tagatgccgc cgatgtgcgc
      361 gggttggctc acatcacggg cggcgggttg atcgacaacg ttccgcgaat cctccccacc
      421 gggcttcgcg ccgacatcga cacgggctcg tggccgactc atgccgtgtt caccttcatc
      481 cagcaagccg gaggcatcga tgatgctgag atgtaccgcg ttttcaacat gggcatcggc
      541 ctcgttgcgg tggttcggga gcaggaggcg ccccgagcca tctcggcggc cgagtcggcc
      601 ggagcccgcg cgttcttgat cggacgcgtt gccgccgaca gcggtgcggg cccacgggtg
      661 gttctccact agccatcacc gcattccgcg aaggagtggt ccggttgccc ggcgagttcc
      721 gtatcggagt tctggcctcc ggccgaggca gcaaccttca atccatcctc gacgcgtgtg
      781 acgacgggcg catcgatggc cgcgtggtcg tggtcgtcag cgatgtggct gaggcctacg
      841 cgctcgaacg cgcccgccgg agagggatcg aggccgtcta ccacgacccg gggcggttcc
      901 ggaccaagct cgagcccgag cgtgaggccg ctctcgcgga gcttctcctc gaccgggagg
      961 tccagctcgt gtgcatggcc gggttcatgc gcgtgatcca cgaacccctg ctgaacgcct
     1021 ttcccggccg gatcatgaac atacacccgt ctcttttgcc cgcattcccc ggcctgcatg
     1081 tgcagcagca ggccctcgac tacggcgtga agattgcggg gtgcacggtc cacttcgcca
     1141 acggaggcgt ggatgccggc cccatcatct gccagggagc cgtcgaggtg gcagaggatg
     1201 ac
//
LOCUS       DAOSPL010001543         1201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_117747_length_1201_cov_2.506981, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001543 DAOSPL010000000
VERSION     DAOSPL010001543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117747_length_1201_cov_2.506981"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PLQ54_21285"
     CDS             complement(<1..783)
                     /locus_tag="PLQ54_21285"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HOS95855.1"
                     /translation="MHSTDPANSLLHFAFVLHNHQPVGNLDRVFRQAFEQAYLPFVEV
                     VEEFPGLRVVLHYTGPLLEWLEAERHEFLDRLRVLAASGQAEILTGAFYEPILSAIPP
                     RDRLGQIRRMTTWVEDNLGVHPRGLWLAERVWEPHLARDLADAGAEYCLIDDAPFERI
                     GIDRDGLHGYYITEEDGRMLRVVPIDSTLRALIPFRLPGETVAYLRDVAAGGSGRLAT
                     FADDGEKLGDWPGTHKWVYQEGYLRDLFTLMEDNASWLKTTTL"
BASE COUNT          220 a          408 c          396 g          177 t
ORIGIN      
        1 gagcgtcgtc gtcttcagcc agctcgcatt gtcctccatc aaggtgaaca ggtcgcgtag
       61 gtacccttcc tggtacaccc acttgtgggt gccgggccag tctccgagct tctcgccgtc
      121 atcggcgaag gtggcaagcc gccccgatcc cccggcggcg acatcacgaa ggtaggccac
      181 ggtttccccc ggcaacctga aggggatcag cgcccgcagg gtcgaatcga tgggcacgac
      241 ccggagcatc cgcccgtcct cctcggtgat gtagtaccca tggagcccat cgcggtcgat
      301 cccgattcgc tcgaagggtg catcgtcgat gaggcagtac tcggctcccg catccgccag
      361 gtcgcgagcc aggtggggct cccacacgcg ctcggccagc caaaggccgc gagggtgcac
      421 gcccaggttg tcctcgaccc aagtcgtcat gcgccgaatc tgcccgagcc ggtcgcgggg
      481 cgggatcgcc gagaggatgg gctcgtagaa cgcacccgtg aggatctcgg cttgcccact
      541 ggcggccaag acgcggaggc ggtcgaggaa ctcgtggcgc tcggcttcga gccactcgag
      601 aagcggccca gtgtagtgga gcacgacccg caagccgggg aactcctcga ccacctcgac
      661 gaacggcagg tacgcctgct cgaacgcctg ccggaacacg cgatccagat tgccgacagg
      721 ctggtggtta tggagaacaa aggcaaaatg cagcagggag ttagccggat ctgtgctatg
      781 cataccggga gtgtagtcca aggcgcccgg gcgtgcgaga gggacccctt cggcccatcg
      841 gcggtaacct gggcccgagc ggtgcgagta aggcccctac accgcactgc cctgcctcgt
      901 ggaggggccg cacagggagg cgcccgaaag ccgcgtaggt cgagcgtcgc gctcaacgcc
      961 tcaacgtcga tcgacaggtc cgcctgcggc cgaccagatc ccgttgagcg cccgccacgc
     1021 tgggggacgc gtggttcgcc ccatgacgcc cttccgcaac ccgacccacg ggccgctgga
     1081 gctccaaacg gccacccatg cccattcggg ccgctttggc tgcgcatcga ggctgagccc
     1141 aagagactgc cggtgatgca acgcggagag gcagatgacc ggaaaaccca ttggcctacg
     1201 c
//
LOCUS       DAOSPL010001544         1199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_117982_length_1199_cov_3.452797, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001544 DAOSPL010000000
VERSION     DAOSPL010001544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117982_length_1199_cov_3.452797"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..>1199)
                     /locus_tag="PLQ54_21290"
     CDS             complement(23..>1199)
                     /locus_tag="PLQ54_21290"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOS95856.1"
                     /translation="DLGPYFAKIAQDTTEAQTPEGLVPDIAPEYVVFSGGFRDSPEWG
                     SACIIAPWISYLFHGDRRVLEDNYETMKRYVAYLGTKANRHIVSHGLGDWYDVGPGPN
                     GPSQLTAIALTATALYYQDIDTLAKIARALGRADDAQTYSALGADVREAFNDAFLHAN
                     EGYYDRDSQTANAMPLVLGLVDPNAREAVLQRLARGIRDRGDRITAGDIGFSYVVRAL
                     TDGDRGDVLYDMIVQDKGPGYIHQLRQGATSLIEAWDANRGASQNHCMLGHAEEWFYR
                     GAAGIRVDESHPGFSHFTLRPQMVRSLDWVRAEYDSVRGTIGSSWTRTGRHIAWTVTI
                     PPNSSATVWVPARGRGSVTEGGRPATGAPGLTFSRFEDGCAVFEAVSGTYRLESVTD"
BASE COUNT          174 a          384 c          438 g          203 t
ORIGIN      
        1 cggccctccc cccggcgccg gcctagtcgg tcacggactc gagtcggtag gtgcccgaca
       61 cggcctcgaa aaccgcgcag ccgtcctcga agcgcgagaa ggtaaggccc ggcgcgcccg
      121 tggccggacg ccccccctcg gtcaccgacc cgcgtccgcg agccgggacc cacacggtgg
      181 ccgagctgtt cggtgggatc gtcaccgtcc acgcgatgtg ccgaccggtg cgggtccagg
      241 agctcccgat ggtcccgcgc accgagtcgt actcggcgcg cacccagtcg aggctgcgga
      301 ccatctgcgg acgcagcgtg aagtgggaga agcccgggtg ggactcgtcc acgcggatgc
      361 ccgcggcacc gcggtagaac cactcttcag cgtggcccag catgcagtgg ttctgggagg
      421 cgcctcggtt cgcgtcccac gcctcgatca gtgatgtggc gccctggcgg agctgatgga
      481 tgtagccggg tcccttgtcc tgaacaatca tgtcgtagag gacatcgcct cggtcgccgt
      541 cggtgagggc gcgtaccacg taggagaagc cgatatcgcc cgccgtgatg cggtcgcctc
      601 tgtctcggat tccccgggcg aggcgttgga gcacggcctc gcgggcgttg gggtcgacga
      661 ggcccagcac cagaggcatg gcgttggccg tctggctgtc gcggtcgtag tagccctcgt
      721 tcgcgtggag gaaggcgtcg ttgaatgcct cgcgaacgtc tgcgccgagg gccgagtagg
      781 tctgggcgtc gtccgcccgg ccgagcgcac gggcgatctt cgcgagtgtg tcgatgtcct
      841 ggtagtagag cgccgtcgcg gtcagtgcga tggccgtgag ctgtgagggg ccgttggggc
      901 ccggccccac atcgtaccag tcgccgaggc cgtggctcac gatgtggcgg ttggccttcg
      961 tacccagata ggccacatag cgcttcatgg tctcgtagtt gtcctcgagg acgcgcctgt
     1021 cgccgtggaa gaggtaggag atccaaggtg cgatgatgca ggcgctcccc cactcgggtg
     1081 agtcacggaa gccgccgctg aagaccacgt actcgggcgc gatgtccgga accaggcctt
     1141 cgggcgtctg ggcttcagtg gtgtcctggg cgatcttggc gaagtagggg ccgaggtcg
//
LOCUS       DAOSPL010001545         1198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_118186_length_1198_cov_2.111986, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001545 DAOSPL010000000
VERSION     DAOSPL010001545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118186_length_1198_cov_2.111986"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..811
                     /locus_tag="PLQ54_21295"
     CDS             2..811
                     /locus_tag="PLQ54_21295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOS95857.1"
                     /translation="MAESVKVAYAAPECMIDQTMHYCPGCTHGIAARLVAETIDELGI
                     RDRAIGISPVGCSVYLYDYIDCDFAQAAHGRAPAVATGLSRALPDCVVFTYQGDGDLA
                     SIGTAEIVHAASRNERMTVVFINNAVFGMTQGQSAPTTLLGQRTSTSPRGRQAKGEGY
                     PLRVSEMLATLEGPTYVARAALGAPKYVTQAKRFMRRAFEAQVIHGGFGMLELLSSCP
                     TYWGMTPAEAMRWLEEGMMAYYPVGEFVDRRDRETSGSVREGGEGLRVSDR"
     gene            833..>1198
                     /locus_tag="PLQ54_21300"
     CDS             833..>1198
                     /locus_tag="PLQ54_21300"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOS95858.1"
                     /translation="MMREILIHGTGGQGALTAGQLLAIASLRDGYTVSVVPHYTPEVR
                     GGEANAILVISSDPIGSVLPAEWDVALLLANKAAARHALVVRPGGVVLYNSSLVPPPA
                     TRDGAVAIGIPASAIAEEAG"
BASE COUNT          185 a          392 c          422 g          199 t
ORIGIN      
        1 catggcggag tcagtgaaag tggcatacgc agcgcccgag tgcatgatcg accagaccat
       61 gcactactgc ccagggtgca cgcatggcat cgccgcccgg ctcgtggccg agaccattga
      121 cgagctgggc atccgcgacc gcgctatcgg catatcgccg gtgggctgta gcgtctatct
      181 gtacgactac atcgactgcg acttcgccca agccgcccac ggtcgggcgc cggcggtggc
      241 gaccgggctg tcgagagcgc ttccggactg cgtggtgttc acgtatcagg gtgacggtga
      301 tctcgcctcc atcggcaccg ccgagatcgt gcacgcggcg tcgcgcaacg agcgcatgac
      361 cgtggtcttc atcaacaacg cggtgttcgg catgacccag ggccagtcgg cgcccacgac
      421 gcttctcggc cagaggactt cgaccagccc gcgcgggcgc caggccaagg gcgagggtta
      481 cccgttgcgc gtgagcgaga tgctggcgac gctcgaaggc cccacctatg tggctcgggc
      541 ggccctgggt gccccgaagt acgtcaccca ggccaagcgc ttcatgcggc gagccttcga
      601 ggcccaggtg atccatgggg gtttcgggat gctcgagctg ctttcgtcgt gcccgacgta
      661 ctggggcatg actccggcgg aggccatgag gtggctcgag gaaggcatga tggcctacta
      721 tccggtcggg gagttcgtcg accggcggga ccgcgagacc agcggctcgg tccgggaggg
      781 gggcgagggt ctgcgtgtct ccgatcggta gcgggcgcgc gggccagtct ccatgatgcg
      841 tgagatcctg atccacggca ccggaggtca aggcgcgctc accgctggac agcttctggc
      901 catcgcctcc ctccgcgatg gctacacggt ctcggtagtc ccccactata cgcccgaggt
      961 ccgtggcggt gaggccaatg ccatcctcgt gatctcgagc gaccccatcg ggagcgtgct
     1021 accggcggaa tgggatgtgg cgctcctcct ggctaacaag gccgcggcca ggcatgccct
     1081 ggttgtgcgc cccgggggcg tcgtgctcta caactcgtcg ctagtgcccc cgcccgccac
     1141 gagggacgga gcggttgcca tcgggatccc ggcctcggcc attgccgagg aggcgggg
//
LOCUS       DAOSPL010001546         1195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_118625_length_1195_cov_2.070175, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001546 DAOSPL010000000
VERSION     DAOSPL010001546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118625_length_1195_cov_2.070175"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="PLQ54_21305"
     CDS             <1..497
                     /locus_tag="PLQ54_21305"
                     /inference="COORDINATES: protein motif:HMM:NF020680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HOS95859.1"
                     /translation="AALSCGRLGLAELMVREPGLRQVRDRVLTILRESWLGGGLGSLR
                     GPEEVHSLCVEWWTRTAGAGAAGELGATGRERAVRAAAPELLAFMEGYIRDVMAFRVG
                     ARSGLQNPDRAEDVAADAQRFSPEGLIARLRALARTRRALAANASSRLALEVALARMR
                     NLPR"
     gene            525..854
                     /gene="trxA"
                     /locus_tag="PLQ54_21310"
     CDS             525..854
                     /gene="trxA"
                     /locus_tag="PLQ54_21310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOS95860.1"
                     /translation="MMAKPTDVTSATFEAEVLQSDLPVLVDFWAAWCGPCRAVAPVVA
                     SIAERYSGRLKVCKVDVDANRDIAEKYEIRGIPTLLFFRGGETAAQHVGLLSESDLAA
                     KVDEVLA"
     gene            1016..>1195
                     /locus_tag="PLQ54_21315"
     CDS             1016..>1195
                     /locus_tag="PLQ54_21315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95861.1"
                     /translation="MRVVPGSGPWRVRAVLPVGLALVLVVAAWLPASAAESVIEALQN
                     EYIRIADSARPSVVVV"
BASE COUNT          160 a          332 c          479 g          224 t
ORIGIN      
        1 cggcggccct ttcgtgcggg cggctggggc tcgcggagct gatggttcga gagccgggtc
       61 ttcgccaggt gcgggaccgc gttctgacga tcctccgcga gagctggctg ggaggcggcc
      121 ttgggtcact gcgggggccg gaggaggtcc acagcctgtg tgtcgaatgg tggacgcgga
      181 cggctggggc tggtgcagcg ggggaactcg gggccacggg acgcgagcga gcggtgcggg
      241 cggcggctcc cgagctgctg gcgttcatgg aggggtatat tcgggatgtg atggccttcc
      301 gcgtgggggc ccgttcgggc cttcagaatc ccgaccgggc cgaggacgtg gccgcagatg
      361 cccagcgttt cagcccggag gggctcatcg cgcggcttcg ggcgttggcc cgtacgcggc
      421 gggctcttgc ggcaaacgcg agctctcggc tggcactgga ggtagccctt gcccgcatgc
      481 gcaatcttcc ccggtgatcg ggtgtaccta gaaagaggag atcgatgatg gccaagccaa
      541 ccgatgtgac gtccgccacc ttcgaggccg aagtgctgca gagtgatcta ccggttctgg
      601 tcgatttctg ggccgcctgg tgcggaccgt gtcgtgccgt ggcgccggtg gtggcgtcca
      661 tagcggagcg gtacagcggc cgcctgaagg tatgcaaggt cgacgtcgac gcgaaccggg
      721 acatcgccga gaagtatgag atccgtggca tccccacgtt gctgttcttc cggggaggag
      781 agactgcggc tcagcacgtg ggtctgcttt cggagtccga cttggcggcc aaggtggacg
      841 aggttctggc ctaggctctc gggtgggtgc ggagggtcgc gacctggcgg gccaaccatt
      901 tgccgccggg atcgtcctaa cgggttgagc tgtgggacga ggaccggttt cttgtgccgc
      961 cggccggcgg cgcaagcccg atggtgaggt ggcaaggatc gaaggaggga tcagcatgcg
     1021 tgtggttccc ggttcgggcc cctggcgggt ccgagctgtt ctcccggttg ggttggctct
     1081 ggttctggtg gtggcggcgt ggttgcccgc gtcggcggcg gagtcggtca tcgaggccct
     1141 tcagaacgag tacatccgga tcgcggactc agcgcgtccg agcgtggtgg tggtg
//
LOCUS       DAOSPL010001547         1193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_118890_length_1193_cov_2.492091, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001547 DAOSPL010000000
VERSION     DAOSPL010001547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118890_length_1193_cov_2.492091"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="PLQ54_21320"
     CDS             <1..579
                     /locus_tag="PLQ54_21320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015172901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HOS95862.1"
                     /translation="PIPTITAAVDARQLSALKAERVAAEEVYGKPQPATSLGDRFVED
                     VRAALYAAKICSYAQGFALLSAASSEFGYHMPLAELARIWKAGCIIRAQFLDRVREAY
                     QAQPQLPNLLISPSFREEVASRASRWRRVIAQAVACGIPVPAMSASMAYFDGYTSSRL
                     PANLLQAQRDYFGAHTYERIDAEGVFHTRWQE"
     gene            complement(590..>1193)
                     /gene="larB"
                     /locus_tag="PLQ54_21325"
     CDS             complement(590..>1193)
                     /gene="larB"
                     /locus_tag="PLQ54_21325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOS95863.1"
                     /translation="VYAQGKTPQQVAQIMQRLSETSESPVLATRASHEAHEAVRAVCA
                     EVEYHELARAIVVGRSRLRRKPNYESAYISVLCAGTSDIPVADEAALTAELLGNRVER
                     VYDVGVAGLHRLMDQVDLLRASACLVVVAGMEGALASVVGGLVNRPVVAVPTSVGYGA
                     SFGGVAALLSMLNTCATGVAVVNIDNGFGAGFFASMVNAG"
BASE COUNT          186 a          459 c          364 g          184 t
ORIGIN      
        1 cccatcccca ccatcaccgc cgcggtcgat gcgcgccagc tgtctgcact caaggcggag
       61 cgcgtggcgg ccgaggaggt gtatggcaag cctcagccag ccacgagtct cggcgaccgg
      121 ttcgtcgagg acgttcgcgc cgcgctgtac gcggcgaaga tctgctcgta cgcccagggc
      181 ttcgcgctgc tctccgccgc ctcgtcggag ttcggctatc acatgccgct cgctgagctg
      241 gcgcgcatct ggaaggcggg ctgcatcatc cgcgcccagt tcctggatcg ggtccgcgaa
      301 gcctaccagg ctcagccgca gctaccgaac ctgctcatca gccccagctt ccgcgaggag
      361 gtcgccagcc gcgcttcccg ctggcggagg gtcatcgccc aggccgtcgc gtgcggcatt
      421 cccgtgcccg ccatgtccgc ctcgatggcg tacttcgacg gctacaccag ctcacgactg
      481 cccgccaatc tcctccaggc ccagcgcgac tacttcggcg ctcacactta tgagcgcatc
      541 gatgccgagg gagtcttcca cacgcgctgg caggagtagc gggggtcacc tatccggcgt
      601 tgaccatgct cgcgaagaac ccggcgccga agccgttgtc gatgttgacg acggccacac
      661 ccgtggcgca cgtgttcagc atcgagagca gggctgccac gccgccgaac gaagcgccgt
      721 aacccacact cgtcggcacg gcaaccaccg gccggttcac cagcccgcca acgacgctgg
      781 ccagcgcgcc ctccatgccc gccaccacca ccagacacgc gctcgcccgc aggaggtcga
      841 cctggtccat cagccggtga aggcccgcca cgcccacgtc gtacacccgc tcgacgcggt
      901 tcccaagcaa ctccgccgtg agagccgctt cgtctgccac cgggatgtcc gacgtgcccg
      961 cgcacagcac gctaatgtat gccgactcgt agttgggttt ccgcctcagc cgactgcggc
     1021 cgaccacgat ggcgcgcgcc agctcgtggt actcgacctc cgcgcagacc gcccggacgg
     1081 cctcatgggc ctcgtgcgac gcccgggtcg ccagcaccgg gctctcggac gtctccgata
     1141 gccgctgcat gatctgggcc acctgctggg gcgtcttgcc ctgcgcgtag acg
//
LOCUS       DAOSPL010001548         1190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_119289_length_1190_cov_3.399119, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001548 DAOSPL010000000
VERSION     DAOSPL010001548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119289_length_1190_cov_3.399119"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1190
                     /locus_tag="PLQ54_21330"
     CDS             <1..>1190
                     /locus_tag="PLQ54_21330"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOS95864.1"
                     /translation="LAARHGTPLYLFDPAALRARAAEFARAFAAVLPSTRVYYAVKSN
                     NHPAVAREVAHQGLGLDVSSGVELAMAIECGATDIVFSGPGKTDAELTLAVEHADRVT
                     VLMDSFAELHRLEHTAGGRGRSVRAGIRITNDERGLWRKFGIPLDQLPRLYDEALRSS
                     YVQLRGLQFHSSWNLDPTAQVAFLERLGQALAALPAGKRRWVEFIDIGGGFWPPSGEW
                     LQRAGTPVGRIEAAALGDGGGLRGRYCLPAQPIETFAEALSSALGEHVFPHIEPTICL
                     EPGRWLSHEAMHLLLSVVDCKSADLAITDGGTNAIGWERFEVDYFPVINLSRPALVAR
                     ECMVLGSLCTPHDVWGDEFFGEMIGPGDVLLIPTQGAYTYSLRQEFIKPIPKVVSVET
                     GEAV"
BASE COUNT          178 a          388 c          428 g          196 t
ORIGIN      
        1 ccctggcggc ccgccacggg acgccactct acctgttcga cccggccgcg ctgcgggccc
       61 gcgcggcgga gttcgcgagg gcgttcgcgg ccgtgctgcc atccacgcgg gtgtactatg
      121 cggtgaagag caacaaccat cccgccgtgg cgcgggaggt cgcgcaccag ggactggggc
      181 tcgacgtgtc gagcggcgtc gagctggcca tggccatcga gtgcggcgcg accgacatcg
      241 tgttcagcgg gcccggcaag acggacgccg agctgacact ggctgtggag catgccgacc
      301 gcgtgaccgt gctgatggac agcttcgcgg agctgcatcg cctcgagcac acggccggcg
      361 ggcgggggcg gagcgtgcgc gccggcatcc ggatcacgaa cgacgagcgc gggctctggc
      421 ggaagttcgg catcccattg gatcagttgc cgcgcttata tgatgaggcg ctgagatcct
      481 catatgtgca gctcaggggg ctgcagttcc actccagctg gaatctcgat cccaccgccc
      541 aagtcgcatt cctcgagcgg ctgggtcagg cactggccgc cctgcctgcc gggaagcgcc
      601 ggtgggtcga gttcatcgac atcggcggag ggttctggcc accctcgggc gagtggctgc
      661 agcgcgccgg taccccggtg gggcgcatcg aggcggccgc gttgggggac ggcggcgggc
      721 tgcggggacg ctattgcctg cctgcccagc ccatcgagac cttcgcggag gccctatcgt
      781 cggcgctcgg cgagcacgtg ttcccgcaca tcgagccgac gatctgcctg gagccggggc
      841 ggtggctctc ccacgaggcc atgcaccttc tgctgagcgt ggtggactgc aagtcggccg
      901 atctggccat caccgacggc ggtaccaacg ccatcgggtg ggagcgtttc gaagtggact
      961 attttcctgt catcaacctg agccgtccgg ccctggtcgc ccgggagtgc atggtcctgg
     1021 gctctctgtg cacgccccac gatgtgtggg gtgacgagtt cttcggcgag atgatcgggc
     1081 ccggcgacgt gctgctgatc cccacgcaag gagcgtacac atacagcctg cgccaggagt
     1141 tcatcaagcc catacctaag gtggtcagcg tcgagaccgg cgaggccgtg
//
LOCUS       DAOSPL010001549         1190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_119351_length_1190_cov_2.318062, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001549 DAOSPL010000000
VERSION     DAOSPL010001549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119351_length_1190_cov_2.318062"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLQ54_21335"
     CDS             <1..275
                     /locus_tag="PLQ54_21335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95865.1"
                     /translation="LQVPGVSEQTLGSYADALFKQFGFDKTKDYYPEKVNLDGTTGYL
                     LREKGRDPDKPATVLAYKGNYLFRFEAAKQRNVLRATDAFKSSEGS"
     gene            279..>1190
                     /locus_tag="PLQ54_21340"
     CDS             279..>1190
                     /locus_tag="PLQ54_21340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95866.1"
                     /translation="MGRLGAYGALALSAAALAALPGGSGAEPLANLLVNPRFDEGVLG
                     PRGWSYNTPEGNRVTWQQDAATGARAVLLEGSGRDWAGLTSTAVDVVPGEILTMAVWA
                     SLRPGPEATGGDHVYVRFFTERGFLGQEGLSLAGLGREWRLLIGRVTVPTASARVDVS
                     VQIRSAAKVLIGAVGLARGDLEGAMAGLIGEEPNGADWRQIDTAQNLPSDADANGLPD
                     TLEAFLGIAPAEGARSLRLTRAKTTSFQTPTGYREDNDLKVDAVIVAGNDESSIRSWA
                     ATGYEPHVMVGFRAGPEYVEQQGHADEV"
BASE COUNT          197 a          375 c          427 g          191 t
ORIGIN      
        1 atctccaagt gcctggtgtc agcgagcaga ctctgggctc gtacgccgat gcgcttttca
       61 agcagtttgg gtttgacaag accaaagact actatcccga gaaggtcaac ctggacggta
      121 cgacgggcta tctgctccga gagaaggggc gcgaccctga caaaccggcc acagtgcttg
      181 cctacaaggg gaactacctg ttccgcttcg aggccgccaa gcagaggaat gtgctgaggg
      241 cgaccgatgc cttcaagtcc agcgaagggt cgtgacccat gggccgtctg ggcgcctatg
      301 gcgcattggc actgagtgcc gcggcgctgg ctgcgcttcc ggggggatcc ggggccgagc
      361 cgctggcgaa cctcttggtg aacccccggt tcgacgaagg cgtgctcggc ccccggggtt
      421 ggtcgtacaa cacgcctgag ggcaaccggg tcacctggca acaggacgct gccactggcg
      481 cgcgcgccgt gctcctggag ggctccgggc gcgactgggc ggggctcacg tcgactgcgg
      541 tggacgtcgt gcccggggag atcctgacga tggccgtctg ggcgtccctc aggccaggcc
      601 ccgaggccac cggcggcgac catgtgtacg tgcggttctt cacggagcgg gggtttctgg
      661 gccaggaagg tctctcgctg gcgggattgg ggcgggagtg gcggctgctc attggacgcg
      721 tgactgtgcc gacggcgtcg gcccgggtcg acgtgtccgt gcagatccgg tctgccgcca
      781 aggtgctgat cggcgccgtc ggcctggcgc gaggcgacct ggagggggcc atggcgggcc
      841 tcatcggtga ggaaccgaac ggcgccgact ggcgtcagat cgacaccgca cagaacctgc
      901 cgtccgatgc ggatgccaat ggcctccccg atacgctcga ggctttcctc ggtattgcgc
      961 ccgccgaagg cgcgcgcagc ctgcggctca cccgagccaa gaccacgagc ttccagaccc
     1021 cgaccggcta ccgcgaggac aatgacctca aggtcgatgc cgtcatcgtc gcgggcaacg
     1081 acgagagcag tatccgcagc tgggccgcca caggctatga gccgcacgtc atggtcgggt
     1141 tccgggccgg tccggagtat gtcgagcagc aggggcatgc ggacgaagtc
//
LOCUS       DAOSPL010001550         1188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_119638_length_1188_cov_2.855252, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001550 DAOSPL010000000
VERSION     DAOSPL010001550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119638_length_1188_cov_2.855252"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..834)
                     /locus_tag="PLQ54_21345"
     CDS             complement(136..834)
                     /locus_tag="PLQ54_21345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOS95867.1"
                     /translation="MRMRGFTLIELLVVIVIIAILAAFLFPVYNKVMAEGAKRACTAN
                     LHQIAVALGQYYQDHHAYPPSPWDGANYNPYGGLVALTKTNLSLPMVCKEDERSASDF
                     VDGVAFSYAYDFEGRNPNVPANIQDRLLYNFWGYTPEGYEAPRSLYYDVAQSGGPVPA
                     PVRKWTEFPFLVNSQAPKTTIITHCPFHRMGDTELVLRLDGSVKAIPRAMQDEANDVT
                     RWEAEGKWPTQPAE"
BASE COUNT          197 a          393 c          377 g          221 t
ORIGIN      
        1 gccgatcgca tgcccggtac cgtctttcgg ccgccgactc ccattgctct caccgtcctc
       61 cggcacccac atcccaccgg ctcggtccgc aaagcgcact cggccaagcg cccctcgacg
      121 ctcgccccgg ccccactact cggcgggctg ggtgggccac ttcccctcag cttcccaccg
      181 agtcacatcg ttggcttcgt cctgcatggc tctggggatg gccttgacac tgccatccag
      241 gcgcaggacc agctcggtgt cacccatacg atggaaagga cagtgggtga tgatggtggt
      301 cttgggcgcc tgggagttga cgaggaaggg aaactcggtc cacttccgta ccggtgcagg
      361 cacggggccg ccagactgag cgacatcgta gtagagcgat cggggggcct cgtagccctc
      421 gggcgtatag ccccagaagt tgtagagcag ccggtcttgg atgttggccg gcacattggg
      481 gttgcgccct tcgaagtcgt aggcgtagct gaaggcgacg ccgtcgacga agtcagaggc
      541 cgaccgttca tcttccttgc agaccatcgg caggctcaga ttggtcttcg tcagcgcgac
      601 caatccaccg taggggttgt agttcgcgcc gtcccacggc gagggcgggt aggcgtggtg
      661 gtcctggtag tactgcccaa gggccacagc gatctgatgg agattcgccg tgcaggcccg
      721 tttggcgcct tcggccatga ccttgttgta gacggggaac aggaaggccg ccagtatggc
      781 gatgatgacg atgaccacca acagctcgat gagcgtgaat ccgcgcattc tcatcgcgcg
      841 cctcctcaat gccggggccc atgccccgca gcgccccggc gcgtatgccg ccgcggcgct
      901 ctcggtcgct tgttgtccct ttcggccgcc cgagtgcggg cttcactgtc ttcctgaatc
      961 gcgccaaaac tcctcgtcga gtaccactca accgggaggc cggcttgggc cggcctcccg
     1021 aaggagtgct gcgggattct gaagccctat ctccctgcac cgccactcga tccaccaccc
     1081 gtgaccgcct tggcttcgga tctcccgcac tgagaggtta cccgaatgcg gcggagagca
     1141 aaccggtcgc gtgcattcag acggggggcg gggggaacag atagcctc
//
LOCUS       DAOSPL010001551         1183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_120374_length_1183_cov_3.885638, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001551 DAOSPL010000000
VERSION     DAOSPL010001551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120374_length_1183_cov_3.885638"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PLQ54_21350"
     CDS             <1..1131
                     /locus_tag="PLQ54_21350"
                     /inference="COORDINATES: protein motif:HMM:NF019193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HOS95868.1"
                     /translation="LAEIPTLARYKMNFVMNCYGSMCDIEHHGWGTPDCNRWWEPLPA
                     AKRAAYERVVSKCQSRGIEFCFSLNPNISSRRAVNYESPEDLELIWQHYEWMLGLGVR
                     WFNIQFDDITQGTDPSGQARLANTLLERLRQRNPRSELILCPVYYWGTGHEEPARPYL
                     ETLARDLHPDVYVFWTGDGVVGRITRKAAESYRTVVGHRLFIWDNYPVNDGAPTLHLG
                     PLMKRDPDLCEVAEGYMSNPMYTQNEANRIPLLTVADYAYNPWAYDPARSIGQAILHQ
                     SRTRSQATVLRDLVELYPGMVLFDEGTGYNPAVDRFEELIDAPHSRHLADMYLRHVTD
                     LALRMRRAFPGHFADTLATLEANLESMRLTYRERYGVEPPAR"
BASE COUNT          217 a          395 c          378 g          193 t
ORIGIN      
        1 ctggcggaga tcccgacact cgcccggtac aagatgaact tcgtgatgaa ctgctatggc
       61 tcgatgtgtg acatcgagca ccatggctgg gggacgccgg actgcaatcg ctggtgggag
      121 cccttgccgg cggcgaagcg cgccgcctat gagcgcgtcg tcagcaagtg ccagtcgcgc
      181 ggcatcgagt tctgcttcag cttgaatccg aacatctcat cgcgccgggc ggtgaactac
      241 gagagccccg aggatctgga gctgatctgg cagcattacg agtggatgct cggcctgggt
      301 gtgcggtggt tcaacatcca gttcgatgac atcacccagg gaaccgaccc ctccggccaa
      361 gcccgactgg ccaacacgtt gctcgagcga ctgcgccaga ggaacccgcg ctcggagctg
      421 atcttgtgtc cggtctacta ctggggcacc gggcacgagg aacctgcgcg gccgtacctc
      481 gagacgctgg cgcgtgatct gcacccggat gtctacgtct tctggacggg cgacggagtg
      541 gtcggccgta tcacgcggaa agcagcggag tcgtaccgga ctgtcgtagg ccaccggctg
      601 ttcatctggg acaactaccc cgtcaatgat ggcgcgccga cgctgcacct cgggccgctg
      661 atgaaacgtg accccgacct gtgcgaggtt gccgaaggct acatgtccaa cccgatgtat
      721 acgcagaacg aggcgaatcg gatcccactg ctcaccgtgg ccgactacgc gtacaacccg
      781 tgggcctacg atccggccag gtccatcggg caggccattc tgcatcagtc gaggacgcgg
      841 agccaggcga ccgtgctccg cgacctggtg gaactctacc cggggatggt cctcttcgac
      901 gagggcaccg ggtacaaccc ggccgtcgac cggttcgagg agctgatcga cgccccgcac
      961 tcccggcacc tggccgacat gtacctgcgg cacgtgacag acctggccct gcggatgaga
     1021 cgcgcattcc ccgggcactt cgctgacacg ctcgccacgc tcgaggcgaa cctcgagtcc
     1081 atgcgcctca cctaccgcga gaggtacgga gtcgagccgc cggcgcgctg accccgccta
     1141 cggcttcccc accggcacga cgcgcgcaag gtgcagcgtc acg
//
LOCUS       DAOSPL010001552         1179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_120996_length_1179_cov_3.188612, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001552 DAOSPL010000000
VERSION     DAOSPL010001552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120996_length_1179_cov_3.188612"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..>1179)
                     /locus_tag="PLQ54_21355"
     CDS             complement(79..>1179)
                     /locus_tag="PLQ54_21355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95869.1"
                     /translation="IVPAADLGKRTCRIDDHSRLLVNGQPFFPLGMYFSGIEEADLRT
                     YADSAFNCLMPYGGATREQMDLAQQLGLKVIYTVKDAYFGTEWCPPEIKRAEDERPYI
                     EARVRQFRGHPALLAWYLNDELPLEYLDRLEAHQQWLEELDPDHPTWVVLYQVDQVAQ
                     YARTYDVIGTDPYPIPDRPARTAGQWARMTKASLGYARPAWMVPQAFNWACYRPTDVD
                     KLRPPTYDELRSMAWQCIAEGANGLVFYSFFDIKRDPAMPFDEHWARLKRLSAEIKEM
                     TPILLSVEGTPAIKAQAADWLHWTVRQQGNTTYLIVVNDEDAQHVARFTLPDVPKEIR
                     ARGEAGTRLGIGGTDLPVAIAPFGVQILEIIF"
BASE COUNT          187 a          393 c          382 g          217 t
ORIGIN      
        1 gaggtgatgc gacatacgcc tctggcattg ggcttaagtg cggaccattc gtgactgtca
       61 ccggtttccg cccgccgctc agaagataat ctccagtatc tgcacaccaa acggcgcgat
      121 cgccaccggg aggtcggtgc ccccgatgcc cagccgggtc ccggcctcgc cgcgcgcgcg
      181 gatctccttg ggcacatccg gcagcgtgaa ccgagcgacg tgctgagcat cctcgtcgtt
      241 cacgacgatc aggtatgtcg tgttgccctg ctgccgcacc gtccagtgaa gccagtccgc
      301 ggcctgggcc ttgatggctg gcgtgccctc gaccgacaac agaatgggcg tcatctcctt
      361 gatctccgcc gagagacgct tcagccgcgc ccagtgctcg tcgaacggca tggccgggtc
      421 gcgcttgatg tcgaagaacg agtagaacac gagcccgttg gcgccctcgg cgatgcactg
      481 ccatgccatg ctccgcagct cgtcgtaggt cggcggcctc agcttgtcga cgtccgtcgg
      541 gcggtagcac gcccagttga aagcttgtgg caccatccac gcgggccgtg cgtagcccag
      601 cgacgctttg gtcatccgcg cccattggcc cgcggtgcgc gccgggcggt cgggtatggg
      661 atacgggtcg gtgccgatca catcatacgt ccgcgcgtac tgcgcgacct ggtcgacctg
      721 gtagagcacc acccaggtcg ggtgatcggg gtcgagctcc tcgagccact gctggtgggc
      781 ctccagccgg tcgaggtact ccagcggcag ctcatcgttg aggtaccatg ccaacagcgc
      841 aggatggccc cggaactggc gcactctggc ctcgatgtac ggtcgctcgt cctccgccct
      901 cttgatctcc ggcgggcacc actcggtccc gaagtaggca tccttgaccg tgtagatgac
      961 cttgagcccg agctgctgag ccaggtccat ctgctcgcgt gtggcgccgc cgtagggcat
     1021 gaggcagttg aaggcgctgt ccgcatacgt gcgcaggtcg gcctcctcga tgcccgagaa
     1081 gtacataccc agcgggaaga acggctgacc gttcaccagc aggcggctgt gatcgtcgat
     1141 gcggcatgtg cgtttcccca ggtcggccgc gggcacgat
//
LOCUS       DAOSPL010001553         1175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_121664_length_1175_cov_2.711607, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001553 DAOSPL010000000
VERSION     DAOSPL010001553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121664_length_1175_cov_2.711607"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1130
                     /locus_tag="PLQ54_21360"
     CDS             228..1130
                     /locus_tag="PLQ54_21360"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOS95870.1"
                     /translation="MDRTTTRRGFLGTLAGAGALAASAGVAGAIEPVVRGKDARMKIS
                     SCAYSYRQFLTGERPSMTLEGFLEICAELELDGVELTSYYFPPDADRDYMLRIRRQAF
                     LLGLDVSGTAVGNRFTGPIGAEHEKQLALVRTWIDHGEVLGAPVIRVFAGNIPEGMTE
                     DDAIALVAESCQACADYGAQHGVMVALENHGGVTATADQVLRIVSRVDSDWFGVNLDT
                     GNFHTADPYVEMAKVAPYTITTHIKTEVRPEGQGPQPVDYGRVVSILREVGYRGYLSF
                     EYEAAEDPMTALPRAVSELRAALA"
BASE COUNT          197 a          365 c          427 g          186 t
ORIGIN      
        1 ggtcggcgcc tgaggcgctg gtgatccgga tccggcatcc acgcgggcgg cagctgaaga
       61 gggtgtggat caacggaacc gaacacaagg gctttgaccc ggcacgcgag tgggtgcggc
      121 tcacgccgag ccgggagcgg atccatgtca gggcggagtt ctgagccgcc ggccgaggtc
      181 cctggcagtg aggccgtgcg ctcggacgga tgggcgggga gtgacccatg gacagaacca
      241 caacgcgacg cggattcctc ggaacgctgg ctggggccgg tgccctggcc gcgtcggcgg
      301 gtgtggctgg ggccatcgag cccgtggtgc gcgggaagga cgcgaggatg aagatctcaa
      361 gctgcgccta ctcgtaccgc cagttcctca ccggcgagag accgtcgatg acattggagg
      421 ggttcctcga gatctgtgcc gagctggagc tcgacggtgt cgagctcacc tcgtattact
      481 ttcctccgga tgccgaccgc gactacatgc tgaggattcg gcggcaagcc tttctgctcg
      541 gccttgacgt ctcgggaacc gccgtgggga accgtttcac gggccccatc ggggcggagc
      601 acgagaagca gctggccctg gtgcgcacct ggatcgacca tggagaagtc ctcggcgctc
      661 cggtcatccg cgtgttcgcc ggaaacatcc ccgagggcat gacggaggac gatgctatcg
      721 cactcgttgc cgagagctgc caggcgtgtg ccgactacgg cgcccagcac ggagtgatgg
      781 tggcactcga gaaccatggt ggggtcacgg ccacagccga ccaggttctc cgtatcgtgt
      841 cgcgggtgga ctccgactgg ttcggcgtga atctggacac ggggaacttc cataccgcgg
      901 acccatacgt tgagatggcc aaggtcgcac cgtacaccat cacgacgcac atcaagaccg
      961 aggtccggcc tgagggccag gggccccaac cggtggacta cggccgggtg gtgtcgatct
     1021 tgcgcgaagt cgggtaccgg ggctacctga gcttcgagta tgaggccgca gaggacccga
     1081 tgacggccct gccccgggcg gtgtccgagc tccgggcggc tctcgcctag cccggaggcc
     1141 ccggcggccc gccgctgccg gtcgggatca gcgcg
//
LOCUS       DAOSPL010001554         1174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_121779_length_1174_cov_2.831993, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001554 DAOSPL010000000
VERSION     DAOSPL010001554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121779_length_1174_cov_2.831993"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /gene="trpS"
                     /locus_tag="PLQ54_21365"
     CDS             complement(<1..992)
                     /gene="trpS"
                     /locus_tag="PLQ54_21365"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOS95871.1"
                     /translation="MEAALTQPAVRGKRILSGIQPTGAPHIGNYFGAIRQYVDLHHEN
                     DGFYFVANYHALTTVRDAETMTRQSLDVALDLLACGLDPARATVFLQSDIPEVMELTW
                     ILTCVTPMGLLERCHAYKDKVSQGLPADHGLFAYPVLMAADILAYDSDYVPVGQDQKQ
                     HLEVTRDIATRLNYAFGQEILKLPEPYIMPQVATVPGLDGRKMSKSYGNDIGIFEDDA
                     SVKRKIMRVVTDSTPVEDPKDPSTCNAFQLFALFATEEERAEMERRYREGGVGYGEVK
                     KQTIELFHRLLDPLRERRRALERDPDFVRDVLRQGAKRARGHAAEVLERARTASG"
BASE COUNT          192 a          364 c          400 g          218 t
ORIGIN      
        1 cccgacgccg tccgtgcccg ctcgagcacc tcggccgcgt ggccgcgcgc ccgcttggcg
       61 ccctgtcgca ggacatcccg gacgaagtcg ggatcgcgct ccagtgctct gcggcgctcc
      121 cgcagtgggt ctagcaatcg gtggaacagc tcgatggtct gcttcttgac ttcgccgtaa
      181 ccgacgccac cctcacggta ccgccgctcc atctctgccc gctcctcctc ggtggcgaac
      241 agggcgaaga gctggaatgc gttgcaggtc gacgggtcct tcgggtcctc gacaggtgtg
      301 ctgtctgtta ccacccgcat gatcttgcgc ttcacgctgg cgtcgtcctc gaagatgccg
      361 atgtcattgc cgtagctctt cgacatcttg cggccatcga ggcctggtac ggtggccacc
      421 tggggcatga tgtagggctc gggaagcttc aggatctcct ggccgaaggc atagttgagg
      481 cgcgtcgcga tgtcccgggt gacctccaga tgctgcttct ggtcttgtcc cacgggaacg
      541 tagtcggagt cgtaggcgag gatatcggcg gccatgagca ccgggtacgc gaacaggccg
      601 tggtcggccg gtaggccctg actgaccttg tccttgtaag catgacagcg ctcgagcagg
      661 cccatgggcg tgacgcacgt caggatccag gtgagctcca tgacctcagg gatgtcggac
      721 tggaggaaca ccgtggcgcg cgccgggtcg aggccacatg ccaggagatc gagggccacg
      781 tcgaggctct ggcgggtcat ggtctcggcg tcgcgcaccg tggtcagcgc atgatagttc
      841 gcgacgaaat agaaaccgtc gttctcatga tgcaggtcca cgtactgccg gatggcgccg
      901 aagtagttgc cgatgtgcgg cgcaccagtg ggctgaatgc ccgagaggat ccgcttgccg
      961 cgcacggcag gctgggtcag agcggcttcc accccaacgc gatcacgtcc ttgggaagat
     1021 gacgataagc ccgtgccacg agggcacagg cttggatgca ccattgtggg ggaggccgag
     1081 gaggcgagtc aagacagggc ccggggcgtg ttgcccactc gccgggtccg ggctcttgtc
     1141 cggctcaagg ccatccgtgc ctgagagggc tcat
//
LOCUS       DAOSPL010001555         1171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_122231_length_1171_cov_3.117384, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001555 DAOSPL010000000
VERSION     DAOSPL010001555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122231_length_1171_cov_3.117384"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..971)
                     /locus_tag="PLQ54_21370"
     CDS             complement(189..971)
                     /locus_tag="PLQ54_21370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95872.1"
                     /translation="MVFLVGSRPAWSEESDQVEAALRGVVQALVSHDRAALEELTDPR
                     AFIGLVADGDTEPLRTDREGMVAQGLAHRLDGLGALEDVQIEVLGELALASWSTRREG
                     AAPVPGYAVLSQQRGLWRANLLALVAESPEAPEAEVPPVSDIVAELVKAGNERDFDKA
                     FARVADGPVVGLAGGPSGGVYAFSDAKELLTMAQAMAPQGLTLDGAVRRHAATRGAGV
                     ALLSLSLSLADQMSFDLSGIVLVVRQGDGWVIPALCVGIVGN"
BASE COUNT          212 a          439 c          346 g          174 t
ORIGIN      
        1 cgctcttcgt ggcgtggtgc gtcaagtcga ccttggcgcg ctacggcggc gccaccgcgt
       61 tccgcaaagc cgtgccattc gccatggggc tggtcatcgg tgacttcatg gctggcagtt
      121 tctggtcact ctacggcacc gccaagggcc tgagggcgta cagcatctgg gtctagtccg
      181 accccacatc agttgcccac gatgcccaca cagagcgcgg ggatgaccca accgtcgccc
      241 tggcggacca cgaggacgat cccgctcaga tcgaacgaca tctggtccgc cagagagaga
      301 gacaggctca ggagggccac acccgctcca cgcgtcgctg catgtcgccg gacggcgccg
      361 tccagagtga gcccctgcgg cgccatggcc tgagccatgg tcagaagctc cttggcatcc
      421 gagaaggcgt agaccccacc cgatgggccg ccggccaggc caaccacagg accgtcggcc
      481 acccgggcga atgccttgtc gaagtcccgc tcgttgccgg ccttcaccag ctcggcgacg
      541 atgtcagaca ccggagggac ctcagcttcc ggtgcctccg gggactcagc aaccagtgcg
      601 agaagattcg cgcgccacaa gcctcgctgc tggctgagta ccgcgtaccc cggcacggga
      661 gcggcaccct cgcggcgggt cgaccacgaa gccagcgcca gctcgcccag cacttcgatc
      721 tgtacgtcct ccagcgcccc caggccgtcg agccggtggg caagcccctg cgcaaccatc
      781 ccctcacggt cggtccgcag tggctcggtg tccccgtcgg ccacaagccc aatgaacgcg
      841 cgcggatcgg tcaactcctc tagagccgcg cggtcatgcg ataccagtgc ctgaacgact
      901 ccgcgtagcg ccgcctcgac ctggtcactt tcctcactcc atgccggccg agagccgacc
      961 agaaacacca tgcaggcccc cacgaacagc gtccgacaca atggccctct ggctccatcc
     1021 caacacatag cacgacacct ccgtagccgg gccgcgatac acccgcccct caaggacgcc
     1081 cgagcgcgag tacggatccc ttcagggcgg taggacacta ggtctgggcc agtatctggt
     1141 ccatcctcgc cgacgtctca cgcagcgtct c
//
LOCUS       DAOSPL010001556         1168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_122732_length_1168_cov_3.036837, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001556 DAOSPL010000000
VERSION     DAOSPL010001556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122732_length_1168_cov_3.036837"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PLQ54_21375"
     CDS             <1..562
                     /locus_tag="PLQ54_21375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="LmbE family protein"
                     /protein_id="HOS95873.1"
                     /translation="HEPLVDDIDIFYDKPLLARVGAVMRDVRPDILLLQSPQDYMEDH
                     MNSSRLGVTAAFCRGMRNFPTDPPRPVFEKEVAVYHALPWGLCDQLRTPILPHFYVDV
                     ADVMAQKRDMLACHKSQKEWLDVSQGLDSYLIAMEDMCAQVGAMSGRFASAEGWRRHS
                     HLGFSAPGFDPMSDALGEWIEPHRPV"
     gene            584..>1168
                     /locus_tag="PLQ54_21380"
     CDS             584..>1168
                     /locus_tag="PLQ54_21380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HOS95874.1"
                     /translation="MARPLSRIAPAWWDFTTLDRELMNDAAALTVEGLQSLSRDGFRV
                     VIHDTLPEFYLAEAMEYIHAWKQSTEDNPVGICGPIGPVDQLPLVAMLVNEMDLSIRS
                     AHFWGMDEWVIDGQVVGEDHPLSFAATDMNLCFNRIRPDLVMPKANIHFPNADPSDYS
                     ASYEGVRCAIMQGGQGEVKHWAFNDPPKREGRYQD"
BASE COUNT          206 a          391 c          372 g          199 t
ORIGIN      
        1 ccatgagccg ttggtcgatg acatcgatat cttctacgac aagccgctgt tggcccgcgt
       61 gggcgccgtg atgcgcgatg tgcggccgga catcctgctc ctgcaatcgc cccaggacta
      121 catggaagac cacatgaact catcgcgact gggcgtcacg gcggccttct gccggggcat
      181 gcggaacttc ccgaccgacc ccccgcgccc cgtgttcgag aaagaggtgg cggtctacca
      241 cgcgttgccc tggggtctgt gcgatcagct ccgcacgccc atcctgccgc acttctacgt
      301 cgatgtggcg gacgtgatgg cgcaaaagcg cgacatgctc gcctgccaca agagccagaa
      361 ggagtggctc gatgtgagcc aggggctgga cagctacctg atcgccatgg aggacatgtg
      421 cgcgcaggtc ggcgccatgt cgggccgttt cgccagcgcc gagggttggc gccggcactc
      481 gcatctcggg ttctcggcgc ccggctttga tccgatgtcg gacgccctcg gtgaatggat
      541 cgagccgcac aggccggtct gaccacattc gaggaggcat accatggcac gaccgctcag
      601 ccgcatcgcc ccggcctggt gggacttcac caccctcgat cgcgagctca tgaacgatgc
      661 cgccgcactg accgtcgagg gtctgcaaag cctctcgcgc gacgggttcc gcgtcgtcat
      721 ccacgacacg ctcccggagt tctacctggc cgaggccatg gagtacatcc atgcctggaa
      781 gcagtcgacc gaagacaacc cggtgggcat ctgcggtccg atcgggcccg tggaccagct
      841 ccccctggtg gccatgctcg tcaacgagat ggacttgagc atccggagcg cccacttctg
      901 gggaatggac gagtgggtca tcgacggcca ggtggtcggc gaggatcatc cgctgtcgtt
      961 cgcggcgacc gatatgaacc tgtgcttcaa ccgcatccgc cccgatctgg tgatgcccaa
     1021 ggccaacatc cacttcccca acgccgaccc gtcggactac tcagcctcgt atgagggcgt
     1081 gcgctgcgcg atcatgcagg gcggccaggg cgaggtgaag cactgggcgt tcaacgatcc
     1141 gcccaagcgc gagggccgct accaggac
//
LOCUS       DAOSPL010001557         1168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_122746_length_1168_cov_2.826595, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001557 DAOSPL010000000
VERSION     DAOSPL010001557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122746_length_1168_cov_2.826595"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PLQ54_21385"
     CDS             complement(<1..714)
                     /locus_tag="PLQ54_21385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95875.1"
                     /translation="MRGSMGLLVALGFACLAGCGAGPDGGDGGPEAGTGAPGDTTELD
                     LGSVRIEAASGIKRLDLPVPDSAEVCFTGYYGAAITRLAEMGPGRLLVSFYPNDGQDY
                     DLWTVDPTKGFASRLASWASSEYNPCWSPDGTKLAFASNMYGSYDIFHMKSTGSSATR
                     ITTQTSSDNAPCWSPDSAKIAFETNRTGTWDVYEMRSSDGANPKALAATAGDERSPAW
                     SPNGNYVAISHNPDRSSGGS"
     gene            881..>1168
                     /locus_tag="PLQ54_21390"
     CDS             881..>1168
                     /locus_tag="PLQ54_21390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95876.1"
                     /translation="MSVPVACCVLLCHALIAPSAGEPVRTLAVDGRYVTLNGQAAFLA
                     GQMAPSAGKTDSLEELQRIVDVMMLPFGMNLWIGGLGGIDYGAYNEVVNIER"
BASE COUNT          189 a          401 c          355 g          223 t
ORIGIN      
        1 agagcctccg ctgcttcggt ctgggttgtg agagatcgcc acgtagttgc cgttgggaga
       61 ccatgcgggg ctccgctcat ccccggcggt cgccgccaac gccttggggt tcgcgccgtc
      121 gctcgaccgc atctcgtaga catcccatgt gcccgtgcgg ttggtctcga acgcgatctt
      181 ggccgaatcc ggcgaccagc acggcgcgtt gtcagacgag gtctgcgtgg taatgcgcgt
      241 cgcggagctg cccgtcgatt tcatgtgaaa gatgtcgtag ctgccgtaca tgttggacgc
      301 gaatgcgagt ttcgtgccgt ccggcgacca gcaggggttg tactccgagg atgcccatga
      361 ggccagacgt gacgcgaacc ctttggtcgg gtcgacggtc cagaggtcgt aatcctggcc
      421 atcatttggg tagaacgaca ccaacagccg cccggggccc atctcggcaa gccgggtgat
      481 ggcggcgccg tagtacccgg tgaagcacac ctcggcgctg tccggcacgg gcaggtctag
      541 tcgcttgata cccgatgccg cctcaatgcg gaccgagccc aggtcgagct cagtcgtatc
      601 ccccggcgct cctgtgcctg cttcggggcc tccgtctcct ccatccggac ccgcgccaca
      661 gcctgcgaga cacgcaaacc caagagctac cagcagtccc atacttccgc gcaaacgccg
      721 cactcccgtt ccccctcggt aggaccattc cggccgtccg gccaatgccg gctcccgctg
      781 ccacaccgcc ctagtctcct acagcattcg ggccccatcg gcactcatcg agtgtcatag
      841 ctactgcata ccgcccggtc tcatctgagg aggccctgtc gtgtccgttc cagtggcctg
      901 ctgtgttctc ctctgccatg ctctcatcgc cccatcggcc ggagagcccg tgaggacact
      961 cgccgtcgat gggcgctacg tgaccctgaa cggccaagct gctttcctgg cgggccagat
     1021 ggcgccttcc gccggcaaga cggactcact ggaggagctg cagcgcatcg tcgacgtgat
     1081 gatgctcccc ttcgggatga acctgtggat cggcggcctc ggcggcattg actacggtgc
     1141 ctacaacgag gtcgtcaaca tcgagcga
//
LOCUS       DAOSPL010001558         1167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_122948_length_1167_cov_2.383993, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001558 DAOSPL010000000
VERSION     DAOSPL010001558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122948_length_1167_cov_2.383993"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLQ54_21395"
     CDS             complement(<1..197)
                     /locus_tag="PLQ54_21395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95877.1"
                     /translation="MTDVTHPQTIGLGIAAVTSQALTVYAWRRRDTLAGQALAAMMAG
                     SALAALAHALSSVGVPPAMRVL"
     gene            398..622
                     /locus_tag="PLQ54_21400"
     CDS             398..622
                     /locus_tag="PLQ54_21400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95878.1"
                     /translation="MDVRVGDPLVQIRVGGLSHDVPAPQLRLTRWASDSAVLSAVARY
                     LDVWVGTLQSKVTVERSPSGNIRVRPAVAA"
     gene            complement(713..>1167)
                     /locus_tag="PLQ54_21405"
     CDS             complement(713..>1167)
                     /locus_tag="PLQ54_21405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95879.1"
                     /translation="APDGRAALVLEEASGQSAVGVKSTATSIVTRRSSILRCRTEWPG
                     RRLIWVDVATHERVEVPVGAGELGGVAWSPEPDGSGHYGFLLAVGGSLVYCGPGGSGP
                     TVVFRSAYEVRDPTWDPHGRNVVFSSPRDATDPSRPSERVANLYAVAL"
BASE COUNT          196 a          417 c          388 g          166 t
ORIGIN      
        1 agcacccgca tcgccggagg caccccgacg gagctgagcg catgagccag cgccgcgagg
       61 gccgaccccg ccatcatcgc cgccaacgcc tggccggcca aggtgtcccg cctgcgccag
      121 gcgtacaccg tgagcgcctg tgaggtgacg gccgcgatgc ccagtccgat cgtctgaggg
      181 tgagttacat cagtcatggc gtccccccgg gccgcagggt cggcagtacc gtcgcagggc
      241 gcctcggacg ccccccatca tactcccacg gattcggtga agagaaccgc aaccgaccgg
      301 ggggctcctg cgtccataga cactgtgcag aggatgggtg cggggccgag gaactccgcg
      361 gggctttgcc cccgggagag ttcaggagga gtctgtgatg gatgttcgag tcggtgatcc
      421 cctggtgcag atccgcgtgg gagggctgag ccacgatgtg cccgctccgc agctccggct
      481 gacgcgctgg gcctcggact cggctgtcct gagcgccgtg gcccgctatc tggatgtctg
      541 ggtcggcacc ctccagtcga aagtcactgt cgaacggagc ccctcgggca acatccgtgt
      601 ccgtccggcc gtggcggcat gacactcacg gactggcgtc gcgccggatg atctcgcttc
      661 cggcggagca gtggcccggc gtggagcccg cacgccaggc cgggacagcc atctatagag
      721 ccactgcgta gaggttcgcg acccgctcac tgggccggct cgggtctgtg gcatcccgag
      781 ggctcgagaa gaccacattt cggccgtggg ggtcccatgt gggatcccgc acctcgtagg
      841 ccgaacggaa caccaccgtt ggcccagacc cgcccggccc gcagtacacc agtgagccac
      901 ccacagcaag caggaacccg taatggcctg aaccgtcagg ctcgggcgac caggcgacgc
      961 caccgagctc cccggctccg acgggtacct cgacgcgctc gtgcgtggcc acgtccaccc
     1021 aaatcagacg acggccgggc cactccgtgc ggcaacgaag gatcgacgaa cgcctcgtca
     1081 cgatcgacgt ggctgtggac ttcacaccca ccgcgctctg cccactagcc tcctcgagca
     1141 ccagggccgc gcggccatcc ggagcga
//
LOCUS       DAOSPL010001559         1163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_123571_length_1163_cov_2.767148, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001559 DAOSPL010000000
VERSION     DAOSPL010001559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123571_length_1163_cov_2.767148"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLQ54_21410"
     CDS             complement(<1..233)
                     /locus_tag="PLQ54_21410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOS95880.1"
                     /translation="MGRIAVLPEDVANKIAAGEVVERPASVVKELVENSLDAGATRVT
                     VYLEDGGKSLIRVVDDGHGMPPDDAYLAVQPHAT"
     gene            complement(319..1005)
                     /locus_tag="PLQ54_21415"
     CDS             complement(319..1005)
                     /locus_tag="PLQ54_21415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95881.1"
                     /translation="MLARPRYGVTLATLLAIWAAVPALGAEPSASQILKRALDLNSGI
                     VDYTARARAEFHAPEGADVPEGQRSMVFDFRVLFKRPNQVKIEANQPVMLPRQLFAFG
                     NLGTTIAREGRVSLVGKRVVDGVPIYNVQVAPPEGTEGAKMLFWIHGAKWTVLRMKMP
                     ASGGNQQSFELDIAWTYARVQGYYVPTRISVSLPGMMFGPNVWGQGRASLTMTDWRIN
                     VGLKDSVFAR"
BASE COUNT          190 a          399 c          378 g          196 t
ORIGIN      
        1 gtggcatggg gctggacggc caggtatgcg tcgtccggcg gcatgccgtg accatcatcg
       61 acgacgcgga tcaggctctt cccgccatcc tcgaggtaca cggtcacccg ggtggcgccg
      121 gcatcgagtg agttctcgac gagctccttg accaccgaag ccggccgctc gacgacctcg
      181 ccggccgcga tcttgttggc gacgtcctcc gggaggaccg ctattctgcc cattcggcca
      241 tccagtccct cgccgcgggg tgggaaccgt gcagatcccc ggggaggggg ctggagctga
      301 ccgcctcctt ggtcctccct acctcgcgaa caccgagtcc ttgagcccca cgttgatccg
      361 ccagtcggtc atggtcaggc tggcccggcc ctggccccac acgttggggc cgaacatcat
      421 gccagggagg ctcaccgaga tgcgcgtcgg gacgtagtag ccctgaaccc gtgcgtacgt
      481 ccacgcgatg tcgagttcga agctctgctg gttccctccc gaagcgggca tcttcatgcg
      541 caacacggtc cacttggcgc cgtggatcca gaacagcatc ttagcgccct cagtgccttc
      601 ggggggcgct acctgcacat tgtagatcgg cacgccatcg accacacgct tgccgaccag
      661 ggagaccctg ccctccctgg cgatggtggt ccccaggttc ccaaacgcga acagctgtct
      721 ggggagcatc acgggctggt tggcctcgat cttcacctgg ttgggccgct tgaacaagac
      781 acggaagtcg aagaccatcg aacgctggcc ttccgggacg tcggccccct cgggggcgtg
      841 gaactcggcg cgcgctctgg ccgtgtagtc cacgatgccg ctgttcaggt cgagggcccg
      901 tttgaggatc tgcgacgccg acggctctgc gccgagcgcc ggaacggccg cccagatggc
      961 cagtagcgtc gccagggtga caccataccg gggtcgtgct agcacaggat gtccctcctc
     1021 cggaacacga gcaacccgcc ggcgaagaag gccgcgcagt agcccgcaag ccagccggcc
     1081 gagctggtga gagccacccg agccgcctgg tccagtcctc gggcgagcgg gatctcgtag
     1141 aggttcatgt gcgtcgtcag caa
//
LOCUS       DAOSPL010001560         1161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_123872_length_1161_cov_3.242315, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001560 DAOSPL010000000
VERSION     DAOSPL010001560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123872_length_1161_cov_3.242315"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PLQ54_21420"
     CDS             complement(<1..889)
                     /locus_tag="PLQ54_21420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95882.1"
                     /translation="MAVLALTAAALIASPIADWSYEYTKLTRSSEEWYGSTFYSGPDW
                     TRVGRHWHHSGEQAASVRVFTAPRAGTVRVTGRVFKLHTEGGDGVWAAIRTGRTVWSC
                     VLEAADSRGFEHDLSVDLEQGQRLCFVVGKRATITCDTTGWDPVVTYADGRTYRASEG
                     FDSGDPVWSYEMERRLPPPAPAGCDPASGEDLFPGVPPEDRSALQWRAADGEGGLLAV
                     SRGLGGEPRTGVALRGLLPGATYLVTEDGGTGRACLDGAQLMSAGLTIALAPGQTRVF
                     RYAPTRSGVEAAPPVARPRP"
BASE COUNT          173 a          427 c          399 g          162 t
ORIGIN      
        1 ggggtcgagg ccgcgccacc ggtggcgcgg cctcgacccc ggaccgcgtg ggcgcgtagc
       61 ggaacacccg tgtctgcccc ggagccagcg cgatggtcag accggcggac atgagttggg
      121 cgccgtccag gcacgcccgg cccgtcccgc catcctcggt cacgaggtac gtggcgccgg
      181 gcaggagccc gcgcagcgcc acgccggtcc gcggctcgcc gccgagcccg cgagagacgg
      241 ccagcaggcc gccctcaccg tccgctgccc gccactgcaa cgccgaccgg tcctccggcg
      301 gtaccccggg gaacaggtcc tcccccgagg cggggtcaca cccagcgggc gccggaggcg
      361 gcaagcgccg ctccatctcg tagctccaca ccggatcgcc cgagtcgaag ccctccgagg
      421 cgcggtaggt ccggccatcg gcgtaggtca ccacgggatc ccaaccggtg gtgtcgcagg
      481 tgatggtggc ccgcttgccg acgacgaagc acaggcgctg gccctgctcc aggtccacgc
      541 tcaggtcgtg ctcgaagccc cgggagtccg cagcctcgag gacgcaactc cagacggtgc
      601 gaccggtccg gatggcggcc cagacgccat cgcccccctc cgtatggagc ttgaacacgc
      661 gtcccgtgac ccgcaccgtg cccgcgcggg gcgccgtgaa gacccgaaca ctcgccgctt
      721 gctctccgct atggtgccag tgacgcccga cccgggtcca gtcgggtccc gagtagaagg
      781 tggagccata ccactcctca ctggatctgg tcagcttggt atactcatat gaccaatcag
      841 ctatcggact ggcgatgaga gccgcggccg tcagggcgag aacagccatt ggcgcgcctc
      901 ctccaggcag ccgcccggct ggtgctcggt ttgtctcggg cggggccttc cggccctcgt
      961 cgagcaggtc cgtggggggc cgggcggaac aaccatggta ccaccaatca tcaccgtcgg
     1021 gtgcccctcg gggcccgact ggagaggacg agtcatccga tggccctaga gccgctcgag
     1081 attggcgtgt tcatcagcgt ggagccggac ccggtggccc agttcgccaa ggtccgcgac
     1141 ttcgggctgc ggtcgtgttt c
//
LOCUS       DAOSPL010001561         1161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_123903_length_1161_cov_2.677215, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001561 DAOSPL010000000
VERSION     DAOSPL010001561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123903_length_1161_cov_2.677215"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..>1161
                     /locus_tag="PLQ54_21425"
     CDS             119..>1161
                     /locus_tag="PLQ54_21425"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HOS95883.1"
                     /translation="MPTVEAVDTFAEFLHIDPAGIEVVRESEPDQEHEPDIAFAGVVR
                     HRPPTEDPWWGPRDAEGMATCRLKVGRLREDGRPYVQGARWQANRMPGDIVVDEAQAE
                     TIAREFLRTYCPFYDEGLQPVPPEVLRRTSRPYWSSFWHRETDTTSLRMNLGMRLDDG
                     SIVYNCWYRTGLTPAAITEEQAKQIAEEQWRAKYPEQELVYEKTSKLLESEFSPTRGP
                     AWMVTYRRGPEGGRTREGGTERVWLTIDAVTGAITRAPNFTPPKLTREEAQRICEEQW
                     LAKWPDEKLELVRSAVWLDCELSPSHGPVWYLPHRRSTEGALPVSDPDLAVCIDGLTG
                     EILRAPNLTKPKLS"
BASE COUNT          200 a          385 c          409 g          167 t
ORIGIN      
        1 gggactgacc gccatgggac caaggaccag tgtcactcga gctctcgtcg tgctgtgggc
       61 ggccgctctt cctgtttcgg cgtgggccca ggggcctccc ttgggagcgg ccccccgcat
      121 gcccacagtc gaggccgtcg acaccttcgc cgagttcctg cacatcgacc cggcgggcat
      181 cgaggttgtc cgcgagtccg agccggatca ggaacatgag cccgacatcg cgttcgccgg
      241 cgtcgtgcgc cacaggccac ccactgagga cccgtggtgg ggaccgaggg acgccgaggg
      301 catggccacg tgccgtctga aggtcgggcg gctccgcgag gacggtcggc cctatgtcca
      361 gggggctagg tggcaggcca atcggatgcc cggcgacatc gtcgtcgatg aggctcaggc
      421 cgagaccatc gcacgggagt tcctccgtac ctactgcccc ttctacgacg aggggctcca
      481 gccggttccg ccggaggtac tccgccgaac gagccggccg tactggtcga gcttctggca
      541 ccgcgagacc gacacgacgt ccctccggat gaacttgggg atgcggctcg acgatggcag
      601 catcgtctac aactgctggt accgcacggg tctgacgccg gcggccatca ccgaggagca
      661 ggccaagcag atcgcggaag agcagtggcg tgcgaagtac cccgagcagg agctggtcta
      721 cgagaagacc tccaagctac tggaatccga gttcagcccg acgcgagggc cggcatggat
      781 ggtgacgtac cgtcgcgggc cggagggggg ccgcacacgc gaggggggta cggagcgcgt
      841 gtggctgacg atcgacgccg taacgggcgc catcaccagg gcccccaact tcacgccccc
      901 gaagctcacg cgcgaggagg cccagaggat ctgcgaggag cagtggctcg ccaagtggcc
      961 cgacgagaaa ctggagctgg tcaggtctgc cgtgtggctc gactgcgagc tgagcccctc
     1021 gcatggcccg gtgtggtacc tcccacacag gcggagcacc gagggcgcgc tgccggtgag
     1081 tgaccccgat ctggccgtct gcatcgatgg cctcacgggc gagatcctgc gcgcgccgaa
     1141 cctgacgaag ccgaagctct c
//
LOCUS       DAOSPL010001562         1161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_123904_length_1161_cov_2.669078, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001562 DAOSPL010000000
VERSION     DAOSPL010001562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123904_length_1161_cov_2.669078"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..>535
                     /locus_tag="PLQ54_21430"
     CDS             156..>535
                     /locus_tag="PLQ54_21430"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HOS95884.1"
                     /translation="MHDESKRLTTSFGSPVPDDQNTMTAGQRGPALLQDVHLIEKLAH
                     FDRERIPERVVHAKGAGAWGYFEVTHDVTDYTCAEFLSAVGKRTETFARFSTVGGEKG
                     SADTARDPRGFAVKFYTEDGNYDFV"
     assembly_gap    536..545
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <546..>1161
                     /locus_tag="PLQ54_21435"
     CDS             <546..>1161
                     /locus_tag="PLQ54_21435"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HOS95885.1"
                     /translation="VGNNTPVFFIRDPMKFPDFIHTQKRNPVTNLPDANMAWDFWSLT
                     PESIHQVTILFSDRGTPDGYRHMNGYSGHTFMWYKANGQYVWVRIHMKTEQGIKTLTR
                     QQATQLAGTDPDYATRDLFDAIARGDAPAWKVHAQIMQPDQADQCRFDPFDITKVWPH
                     GEFPLVPFGRLVLDRNPANYFAEVEQAAFSPGNLVPGIAPSPDKM"
BASE COUNT          244 a          381 c          339 g          187 t
ORIGIN      
        1 cacactgccc aactggacgg gaactcctag tggcgcgcag atcgctatta ctcataacag
       61 acgataacgc tcgctgcatt ccggagcaac agtgcaagtt gcgggcctga ggagacgggc
      121 gacagtgacg cgcccagccc atgaggaggt cgatgatgca cgacgagagc aagcggctga
      181 cgacgagctt cggctccccg gtacctgacg accagaacac catgacggca ggacagcgtg
      241 gcccggccct tctgcaggat gtgcacctga tcgagaagct ggcccatttc gaccgagaac
      301 gcatccccga gcgggtggtc cacgccaaag gagcgggagc gtggggctac ttcgaggtca
      361 cgcatgatgt gacagactac acctgcgcgg agttcctctc ggccgtgggc aagcggaccg
      421 agacgttcgc ccgcttctcg accgtcgggg gagagaaagg ctcggccgac acggcacgcg
      481 atccccgagg gttcgccgtg aagttctaca ccgaggatgg gaactacgac ttcgtnnnnn
      541 nnnnncgtcg gcaacaacac cccggtgttc ttcattcggg atcccatgaa gttcccggac
      601 tttatccaca cccagaagcg caacccagtt accaaccttc ccgatgccaa catggcgtgg
      661 gacttctggt cactcacacc cgagtccatc caccaggtca cgatcctgtt ctccgaccga
      721 ggcacgccgg acggctaccg ccacatgaac ggctacagcg ggcacacgtt catgtggtac
      781 aaggccaatg gccagtacgt ctgggtgcgg atccacatga agactgagca gggcatcaag
      841 acgctgaccc gccagcaagc cacgcagctt gctggcaccg atccggacta cgccaccagg
      901 gacctgttcg acgctatcgc ccggggcgat gccccggcat ggaaggtcca cgctcagatc
      961 atgcagcccg accaggccga ccagtgccgg ttcgacccgt tcgacatcac gaaggtgtgg
     1021 ccacatggcg agttccctct ggtccccttc ggccggctcg ttcttgaccg caatccggcc
     1081 aactacttcg ccgaggtcga acaggccgcg ttctcgccag gcaacctggt gccgggcatc
     1141 gctccatcac ctgacaagat g
//
LOCUS       DAOSPL010001563         1160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_124105_length_1160_cov_2.195475, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001563 DAOSPL010000000
VERSION     DAOSPL010001563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124105_length_1160_cov_2.195475"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1160
                     /locus_tag="PLQ54_21440"
     CDS             <1..>1160
                     /locus_tag="PLQ54_21440"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95886.1"
                     /translation="AQAALLILVAAAPFSAMIGLTFPLGARLYAGSIEADARPVGRLY
                     AAEAVGFLVGGLAYTFVLLTRLSAFGVLEVVMAVLCAAMVSVCVGLHRGKPCPAFLGV
                     IAALAMVLGGAVGWRSAEWDQATRMGRWAGLAPSPGCRLVRVVDTPYQNVMLSEYDGL
                     YTVYANGQTCVSFPDPYQSGLEANFVLLQHPSPRRVLLLGWGAEGLIQEMLKSPLELI
                     DYVQVDEGLLRAVEAFLAPGDRAALASPRVHVHHVDARVYVQHARDRYDVVFANVGDP
                     STAALNRYFTTEFFEEVRRILAPGGVFATRVSSAVNVVGEEIGLHTGAVYHSLRQVFP
                     HVLVTWDTANYLFATEERGVLSADPQVIGRRFQKRGIITEYFSDAHPEILLP"
BASE COUNT          163 a          413 c          357 g          227 t
ORIGIN      
        1 gcccaggctg ctctgctcat cctggtggcg gcggcaccct tctcggccat gatcggcctc
       61 actttccccc tcggggcccg cctctacgcc gggagcatcg aggccgacgc gcgtccggtg
      121 ggccgcctct acgctgccga ggccgtcggg ttcctggtcg gcggcctcgc gtacaccttt
      181 gtgctgctga ctcgcctgtc agccttcggc gtgctcgaag tggtgatggc cgtcctctgc
      241 gcggccatgg tctccgtgtg tgtcggactg catcgcggca agccatgccc ggccttcctg
      301 ggcgtcatcg cagcgctcgc catggtgttg gggggcgccg tgggctggcg ctcagcggaa
      361 tgggatcaag ccacccgcat gggccgctgg gccggactgg cgccttcccc tggttgtcgc
      421 ctggtccgcg tcgtcgacac tccctatcag aacgtcatgc tgtcggagta cgatgggctc
      481 tacaccgtct atgccaacgg ccagacctgc gtgtcatttc ccgatcccta tcagtccggc
      541 ctcgaggcga acttcgtgct cctgcagcac cccagtccgc ggcgcgttct cctgctgggc
      601 tggggcgccg aggggctcat acaggagatg ctcaaaagcc ccctggagct tatcgactac
      661 gtgcaggtcg acgagggcct gctgcgggcc gtcgaagcgt tcctcgcgcc gggcgaccgc
      721 gccgctctcg ccagtccacg tgtccatgtg caccatgtcg acgcacgcgt ctacgtacaa
      781 cacgcccgtg accggtacga tgtcgtcttc gccaacgtcg gcgacccatc gacggccgca
      841 ctcaatcgct acttcacgac cgagttcttc gaggaggtcc gacggatcct cgcccccggc
      901 ggcgtgttcg ccacgcgcgt ctcatcggcc gtcaatgtgg tgggtgaaga gatcggcctc
      961 cacaccggcg ctgtctacca ctccttgcgg caggtcttcc cgcatgtgct cgtcacatgg
     1021 gacacggcca actacctttt cgccaccgag gaacgcggtg tcctcagcgc cgacccgcaa
     1081 gtgatcgggc ggcgttttca gaagcgaggc attataacgg aatacttcag cgacgctcac
     1141 cccgagatcc tgctccccga
//
LOCUS       DAOSPL010001564         1156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_124732_length_1156_cov_2.811081, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001564 DAOSPL010000000
VERSION     DAOSPL010001564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124732_length_1156_cov_2.811081"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="PLQ54_21445"
     CDS             <1..974
                     /locus_tag="PLQ54_21445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOS95887.1"
                     /translation="GSDTGGSIRQPASFCSLVGLKPTYGRVSRYGLVAFASSLDQIGP
                     LTKDVRDTALLMNLLAGHDPMDSTSADRPVPDLTELLGRDIRGIRIGVPKEFFAEGTD
                     PEVANAVHAAIRQLESLGAIVGEASTPTVSYGLPTYYIIAPSEASSNLARFDGVRYGH
                     RTSKPVADNVELYEQSRAESFGPEVQRRIMIGTYALSAGYYDAYYLKAQKVRTLLKRD
                     FDKVFEQFDIIAWPTSPCLPFRFGERGDPLSMYMADVCTIPVNMVGIPAVSLPCGFSS
                     GGLPIGIQLGASYFEEPKLLQVAYAYEQSAGFHARRAPLGASCGGEA"
     gene            974..>1156
                     /locus_tag="PLQ54_21450"
     CDS             974..>1156
                     /locus_tag="PLQ54_21450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HOS95888.1"
                     /translation="MALEAIIGLEVHAQLLTRSKMFCRCSTDPSAGPNTVVCPICTAM
                     PGVLPVINRRAVELVVR"
BASE COUNT          186 a          414 c          352 g          204 t
ORIGIN      
        1 tcggttcgga cacgggcggg tccatccgcc agcccgcgtc cttctgtagc ctcgtgggcc
       61 tcaagccaac ctacggccgc gtctcccgct atgggctggt ggccttcgct tcctcactcg
      121 accagatcgg ccccctcacc aaggatgtgc gcgacacggc cctgttgatg aacctgctgg
      181 ccgggcacga tcctatggac tccacctcgg ccgaccggcc cgtgcccgat ctgaccgagc
      241 tgctggggcg cgacatccgc gggatccgga tcggcgtccc caaagagttt ttcgccgaag
      301 gcaccgaccc ggaagtggcc aacgccgtcc acgcggccat ccgccagctc gagagcctgg
      361 gcgccatcgt cggcgaagcg tccaccccca cggtgagcta cgggctgccc acctactaca
      421 tcattgcgcc ctccgaggcc agctcgaacc tggcccgttt cgatggcgtc cggtacggcc
      481 atcggacctc gaaacccgtg gccgacaacg tcgagctcta cgagcagagc cgcgctgaga
      541 gcttcggacc cgaggtgcag cgccgcatca tgatcggcac ctatgcgctc agcgcaggct
      601 attacgatgc gtactatctg aaagcccaga aggtccgcac tctgctcaag cgcgacttcg
      661 acaaggtctt cgagcagttc gacatcatcg cgtggcccac atcgccctgc ttgcccttcc
      721 ggttcggcga gcgcggcgac ccgctatcga tgtacatggc cgatgtatgc accattcccg
      781 tgaacatggt gggcattccg gccgtcagcc tgccctgcgg gttctcgtcg ggcgggttgc
      841 ccatcgggat ccagttgggc gctagctact tcgaggagcc caagctcctc caggtggcct
      901 atgcctatga gcagagcgcc gggttccatg cccggcgagc cccgctgggc gcatcgtgcg
      961 gaggtgaggc gtaatggcgc tggaagccat cattggcctg gaggtgcacg cccagctcct
     1021 cacgcggagc aagatgttct gccgatgctc gaccgacccg tcggccggcc ccaacacggt
     1081 ggtctgcccc atctgcaccg ccatgcccgg agtgctaccc gtcatcaacc gccgtgccgt
     1141 ggagctggtg gtccgc
//
LOCUS       DAOSPL010001565         1155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_124964_length_1155_cov_1.943636, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001565 DAOSPL010000000
VERSION     DAOSPL010001565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124964_length_1155_cov_1.943636"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1155)
                     /locus_tag="PLQ54_21455"
     CDS             complement(<1..>1155)
                     /locus_tag="PLQ54_21455"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS95889.1"
                     /translation="ARFRALAAGAALAGFLLLLGCAANAANLMFVQAVYRRGEIALRA
                     ALGASSLDLTRLVAAELVLIAGCSWGAGLLIAMGILECCTALMPSEYVSLGSPRVDLR
                     VVLFSAGVLVAMLAASAAPALSTVLMTGVQRGRRSRLIGQGGALGVARHVLAGVQFAI
                     TMIFLTGAALLIRSYATLMSVDTGFSASTTILSASYPPSRPREAHGADVRRTLEDIRL
                     LPGVGAAGASIGTLMDEMDIVSFLRVAGRTPDRDILVKHVSAGYFQAMGTELLAGRDF
                     TPDDGQQAAVMIVNQMLAQKYWAGDVPLGDTAFVGARPKRIVGVVQTTRDVALDGAPR
                     PTIFLPLIDPHPAMPVHYSVRTSGGVNLSQIRAAMHRVQPDVVIVDESTVR"
BASE COUNT          228 a          382 c          359 g          186 t
ORIGIN      
        1 cggactgtcg actcgtccac tatcaccacg tctggctgga cccggtgcat cgccgccctg
       61 atctgactca ggttcacgcc tcccgacgta cgcacgctgt aatgaacggg catggccgga
      121 tgcgggtcga tgagcggcag gaagatggtt ggccggggag ccccgtccaa ggcgacatct
      181 cgtgtggttt gcactacacc gacgattctc ttgggcctcg ccccgacgaa cgccgtgtct
      241 cccaacggca cgtcccccgc ccagtatttc tgggcgagca tctggttgac gatcatcacg
      301 gccgcctgct ggccgtcatc gggcgtgaag tcacgtccag cgagaagctc ggtacccatt
      361 gcttggaagt aaccggcgct gacgtgcttt acgagaatat cccggtcagg ggttcgaccg
      421 gcgactcgga gaaagctcac gatgtccatc tcgtccatga gggttccgat cgacgccccc
      481 gctgctccga cacccggaag tagccggatg tcctccagag ttctgcgaac gtctgctccg
      541 tgcgcttcgc gcggccggga aggggggtac gaagccgata ggatggtggt cgaagcactg
      601 aagccggtgt cgacgctcat cagggtcgca taactgcgga tcaacaatgc ggcgccagtc
      661 aggaagatca tggtgatcgc gaactgcaca ccggccagaa cgtgccttgc cacacctaag
      721 gccccaccct ggcctatcag gcgcgaccgt cgacctctct gaacacccgt catgagtacc
      781 gtgctgagtg cgggagccgc agaagcggcc aacatggcga ccagcacgcc agcgctgaag
      841 aggacgacgc ggagatcgac gcgcgggctc ccgagtgaga cgtattcgga gggcatgagg
      901 gccgtacagc actctagaat gcccatggcg atcaggaggc ccgctcccca gctgcagccg
      961 gctatgagca ccaactcggc cgctaccagc cgggtcagat ccagagagct cgcgcccagc
     1021 gccgccctca gcgcgatctc tcctcgccgg tagacagcct gaacgaacat caggttagcc
     1081 gcgtttgcgg cacaccccag caggagcagg aagccggcga gagcggcccc ggccgccaac
     1141 gctcgaaacc gtgca
//
LOCUS       DAOSPL010001566         1154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_125031_length_1154_cov_3.976342, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001566 DAOSPL010000000
VERSION     DAOSPL010001566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125031_length_1154_cov_3.976342"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            371..>1154
                     /locus_tag="PLQ54_21460"
     CDS             371..>1154
                     /locus_tag="PLQ54_21460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95890.1"
                     /translation="MSVRRWICLAAPLGGVLVNSGATAASPLLPLRNASFDAGTPTQD
                     GALPGWQIAVYGAQPEVALDEPAPGLRGNSLRIAASDASDTAIYQDVAVQAGAWYQLT
                     AWVRTERLKADTPIYGAVCACDGPAPFRSSPNRAGTTPWREETICFRAPASGLLRVAL
                     FLVGFGKGTGTVWFDGLRLERADVQQAAPRRIVVCAERLCAEPVDRRIYGNFVEFLDH
                     HVQGMRAQMLDDVSFEGILPPAEHVFWQKDKDVEDHPWRATGD"
BASE COUNT          172 a          367 c          404 g          211 t
ORIGIN      
        1 atggaagtgc cattcaccgt tgagtgaccg ataccatggg gaagcctcac gcacaccatc
       61 gaccgcctgc acgcggacag ggtatggcac gagggtggcg cggtcgggct ccttgtttct
      121 cgataggacc gaaggatctt gccagtcgtg cgccatccga ttgacctccg aacagaccgt
      181 ggggcgcggt cctcgggtgc aaccgcccga tctgccgcac cgtcgccagt gtattccggc
      241 gtaggccccg aggcctcctc cggcgatgtt ggggtgccgc atggcgggcc gcggtggagg
      301 cgagatggct cgtctcgtgg aacgggcgtg cctccctctg cgattgccat agctcctgga
      361 ggtgctggac gtgtctgtac gacgttggat ctgcctcgcg gcgccgctgg ggggagtcct
      421 ggtcaactcc ggcgccactg ccgcttcgcc cctcctgccc ctgcggaatg cgagcttcga
      481 tgccggcacg ccgacgcagg atggggctct gcccgggtgg cagatcgcgg tctacggcgc
      541 tcagccggag gtggccctcg acgagcccgc cccgggtctg cgcggcaact cgctgcgaat
      601 cgcggcctca gatgcctcgg acaccgccat ctaccaggac gtcgcggtcc aagccggtgc
      661 ttggtaccag ctcacggctt gggtgcgtac cgagcgtctg aaggccgaca ccccgatcta
      721 tggggcggtg tgtgcctgcg atggacctgc gcccttcagg agctcaccga atcgagcggg
      781 aacgacgccg tggcgtgagg agaccatctg ctttcgagcg ccggcctcag ggctgcttcg
      841 cgtggcgctc ttcctcgtgg ggtttggcaa gggcaccggg actgtgtggt tcgatggctt
      901 gcggctggag agagccgacg tccaacaggc ggccccgagg cggatcgttg tgtgcgccga
      961 gcgtctctgc gccgagcccg tcgacaggcg gatctacggc aacttcgtcg agttcctcga
     1021 tcatcacgtg cagggcatgc gggcgcagat gcttgacgac gtgagtttcg agggcatctt
     1081 gccccccgcc gaacatgtgt tctggcagaa ggacaaggat gtcgaggacc atccttggcg
     1141 tgcgacgggc gaca
//
LOCUS       DAOSPL010001567         1152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_125405_length_1152_cov_2.779398, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001567 DAOSPL010000000
VERSION     DAOSPL010001567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125405_length_1152_cov_2.779398"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1152
                     /locus_tag="PLQ54_21465"
     CDS             <1..>1152
                     /locus_tag="PLQ54_21465"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOS95891.1"
                     /translation="CPTAVPRHNSCQPQLVLRIREYSLEGRTSCGWQELCRGTAVGHK
                     RIDRFAPVWVSAIRLRCTRSAATPVIRRLAAYYVGLCEPRGGALRWTFDRDEVGAAAG
                     LYRAEPRGARLVAGIIGDALQLDGHGAHVRLGDVPIPEGDFTLAAWICPRTVTRREQI
                     IVAKERSGVADHQFRLYLAPGNRVGFIHSDGEGHGAWPSAGVGDALPARQWSHVAVTR
                     GPSGYTAWVNGQAVATAPPSGSIRHRNTLELRAGARYAPWGDGADSPFDGLIDEVILA
                     PRALSEAELQPSPAARALMSQPVEMTPMQRGPLAKPTPQQAEWHDLEVGMFIHFAPNT
                     WQNQEGDDLSTPLDQINPERLDTDQWVSVAESMGARYIVFVAKHVGGFC"
BASE COUNT          175 a          394 c          407 g          176 t
ORIGIN      
        1 tgtgtcccac ggccgtgcca cggcacaact cctgccagcc gcagctggtt ctgcgcatcc
       61 gcgagtactc gctggagggc agaaccagct gcggctggca ggagctgtgc cgcggcacgg
      121 ccgtgggaca caagcggatc gaccgcttcg ccccggtctg ggtctcggcg atccggctgc
      181 gctgcacgcg atcggctgcg acgccggtca tccggcggct agcggcctac tacgttggtc
      241 tgtgtgaacc acgcggtggg gcgctgcgat ggacctttga ccgcgacgag gtcggcgcgg
      301 cagccggcct ctaccgggcc gagccgcgcg gcgcgcggct cgttgcgggc attatcggcg
      361 acgccctgca actggacggc catggtgccc atgtccgcct tggcgatgtt ccgattcccg
      421 agggagactt caccctggcc gcgtggatct gcccgcgcac cgtgacgcgc cgcgagcaga
      481 tcattgtggc caaggagcgg agcggcgtcg ccgaccacca gttccgcctg tacctggcgc
      541 caggcaaccg ggtggggttc atccactcgg atggggaagg ccacggcgcg tggccctctg
      601 ccggagtggg ggacgcgcta ccggcgaggc agtggtcgca tgtggcggtg acgcggggtc
      661 cgtccgggta caccgcctgg gtgaacggcc aggccgtggc gaccgcgcct ccctcgggca
      721 gcatccggca ccgcaacacg ctggagctgc gcgccggagc gcgctacgcc ccatggggcg
      781 acggcgcgga cagcccattc gacggcctga tcgacgaggt cattctggcg ccgcgggctc
      841 tgtcggaagc agaacttcag ccatctccgg ccgcccgggc gctcatgtcg caaccggtgg
      901 agatgacgcc catgcagaga ggcccactgg ccaagcccac gccgcagcag gcggagtggc
      961 acgacctcga agtggggatg ttcatccact tcgctcccaa cacgtggcag aaccaggagg
     1021 gcgacgacct gtcgacgccg ctcgatcaga tcaaccccga acgactggac accgaccagt
     1081 gggtctccgt cgctgagtcc atgggggccc ggtacattgt cttcgtcgcc aagcacgtcg
     1141 gtggattctg tt
//
LOCUS       DAOSPL010001568         1151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_125568_length_1151_cov_3.332117, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001568 DAOSPL010000000
VERSION     DAOSPL010001568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125568_length_1151_cov_3.332117"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1151
                     /locus_tag="PLQ54_21470"
     CDS             <1..>1151
                     /locus_tag="PLQ54_21470"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOS95892.1"
                     /translation="NYTTRFPYDTFTWRGLDGSAVITHLNVHNMWLTPESMADTWNFL
                     QHKDLEDRRFCCYGYGDGGGGATMEMLEAARRMADLEGCPRTSQITLTEFMRGIERDM
                     PHLPEWVGELYLELHRGTLTSIAKVKRGNRKAEFALRDAEFLSTLAALRGVPYPAEPL
                     LALWKELLTDQFHDILPGSSIAQVNDEAVESFGRIAAGAGRLSEQALRKLTGARRGSG
                     KAAFVVNSLSWDRDGELDLSGIPESMHPADSALQSQWITDIDDAPHLLVSGVTVTGLG
                     ATVIPLAAGDPPSNPSPFRIHGDAVSTPHALVEFDADGRICSLIDKASGREIVAPGGA
                     LNTFLLGEDIPLAWDNWDIDYDQAMKMQPDMRLKHREFVADGALQLRVR"
BASE COUNT          205 a          393 c          352 g          201 t
ORIGIN      
        1 aactacacca cccggttccc ctatgacacc ttcacttggc gtggcctcga tgggtcggcc
       61 gtcatcaccc atctcaacgt gcacaacatg tggctgacgc ccgagagcat ggccgacacc
      121 tggaacttcc tccagcacaa ggatctggag gaccgccgtt tctgctgcta tggctacggc
      181 gacggcggcg gtggtgccac catggagatg ctcgaggccg cacgccgtat ggccgacctc
      241 gaagggtgcc cgcgcaccag tcagatcaca ctgaccgagt tcatgcgcgg catcgagcgc
      301 gacatgcctc atcttccaga atgggtgggg gagctctacc tggagctgca ccggggcaca
      361 ctcacgtcga tcgccaaggt gaagcgcggc aaccgcaagg cggagttcgc cctccgcgac
      421 gccgagttcc tctccacatt ggcggcgctc cgaggcgtcc cctaccctgc cgagccacta
      481 ctcgctctat ggaaagaact cctgaccgac cagttccacg acatcttgcc cggctcatcc
      541 attgcccagg tgaatgatga agccgtcgag tccttcggca ggatcgccgc cggcgccgga
      601 cggttgtccg aacaggcgct gaggaagctc accggagcgc gccgcgggag cggcaaggcc
      661 gcctttgtcg tgaactcgtt gagctgggac cgggacggcg aactggacct ctcgggcatc
      721 cccgagagca tgcatccggc cgactccgct cttcagagtc agtggatcac cgacatcgat
      781 gatgctcctc acctgctggt gtcgggcgtc acggtgacgg gcctgggcgc gaccgtgatc
      841 cccttggccg ccggcgaccc accgtcgaac ccgtcgcctt tccgaatcca tggcgacgcc
      901 gtgtcgaccc ctcatgcgct ggtggagttc gatgccgatg gccgcatctg ctcgctgatc
      961 gacaaagcct cgggacgcga gatcgtggcc ccgggcggcg ccctgaacac tttcctgctg
     1021 ggtgaggaca ttccactggc ctgggacaac tgggatatcg actacgacca ggccatgaag
     1081 atgcagcccg atatgcgcct caagcaccgc gagttcgtcg cggacggcgc cctacagctt
     1141 cgcgtgcgtt g
//
LOCUS       DAOSPL010001569         1150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_125728_length_1150_cov_3.431963, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001569 DAOSPL010000000
VERSION     DAOSPL010001569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125728_length_1150_cov_3.431963"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="PLQ54_21475"
     CDS             complement(<1..1136)
                     /locus_tag="PLQ54_21475"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOS95893.1"
                     /translation="MAREDPAACAHCGSAAIRQDEDVLDTWFSSGLWPFSTMGWPDET
                     EDLRFFFPTSCLVTGYDIITFWVVRMVTLSLYFTGEIPFADVFIHGLVRNEQGKKISK
                     SLGTAIDPMDLVDEWGADSLRFALAALITHGQDIKFSHDRLAAARTFCNKIYQASRFA
                     LMNLEDFDPDAPAPDRAELGLAERWILSRLRHVCEMTTQRVRAYDFGDLAMGLYEFIW
                     GELCDWFLEMAKPSLHDKANGPKRRATQWTLWTAMHHTYRLLHPLMPFLSEELWHALP
                     GTTGSVVVAPWPEPPAEWQDPQAEEAMRAQMDMAYGIRALRAQAEVPPQRTVTASVTS
                     SSETTIAAVRAMLPTMEHILRARINVIPLGSAPPPQSLSLASGST"
BASE COUNT          199 a          372 c          389 g          190 t
ORIGIN      
        1 gtgctcccgc tggccagcga gagggactga ggcggcggag cgctccccag cgggatgacg
       61 ttgatccggg cacgcaggat atgctccatg gtcggtagca tcgcccggac ggccgcgatg
      121 gtggtctcgg agctagaagt taccgacgcc gtcaccgttc tctgcggcgg cacctcagct
      181 tgcgcccgca gcgcgcggat gccgtacgcc atgtccatct gagcacgcat agcctcctca
      241 gcctgcgggt cctgccactc ggcgggcggc tcgggccacg gcgcgacgac gacgctgcct
      301 gttgtgcccg gcagggcgtg ccacagctcc tcggagagaa acggcatcag ggggtgcagc
      361 aggcggtagg tgtggtgcat ggccgtccag agcgtccact gggtggcgcg gcgcttgggt
      421 ccgttcgcct tgtcatgcag gctcggctta gccatctcga ggaaccagtc gcagagctca
      481 ccccagataa actcgtacag ccccatggcc aggtcgccga agtcatacgc gcgcacccgc
      541 tgagtggtca tctcgcagac atggcgcagc cggctgagaa tccagcgttc ggccaggccc
      601 agctcggcgc gatcgggcgc cggcgcgtct gggtcgaagt cctccaggtt catcagcgcg
      661 aaccggctgg cctggtagat cttgttgcag aaggttcgcg cggcggccag gcggtcgtgg
      721 ctgaacttga tgtcctgtcc atgggtgatc agtgccgcca gagcgaagcg gagtgagtcg
      781 gccccccact cgtccacgag gtccatgggg tcgatggccg tccccagcga cttgctgatc
      841 ttcttcccct gctcgttgcg gacgagacca tggatgaaca catccgcgaa ggggatctct
      901 ccggtgaagt agagggacag cgtcaccatc cggacgaccc agaaggtgat aatgtcgtag
      961 cccgtgacga gacacgacgt ggggaagaag aaccggaggt cctccgtctc atcgggccaa
     1021 cccatggtcg aaaagggcca caaacccgac gagaaccagg tgtcgagcac atcctcgtcc
     1081 tgccggatgg ccgccgaccc gcagtgcgcg catgccgccg ggtcctcgcg cgccaccgtg
     1141 atctcgtgga
//
LOCUS       DAOSPL010001570         1147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_126264_length_1147_cov_2.645604, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001570 DAOSPL010000000
VERSION     DAOSPL010001570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126264_length_1147_cov_2.645604"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1147)
                     /locus_tag="PLQ54_21480"
     CDS             complement(<1..>1147)
                     /locus_tag="PLQ54_21480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95894.1"
                     /translation="EEAFLRSTELPEPSAELDTVRAEFLRVIAEAYFGEACRALREAD
                     PDHMVIGCRFAGRTPPEVLEAAGRHNDIFTLNSYPYVDMDRGQVLDVPQQLCDYFDHA
                     GKPMIITEWSFPALDSGLPCTAGAGMRVDTQEQKARCYEIFANMVADLPFMVGQHYFM
                     WVDEPALGISSTFPEDSNYGLVNERDEPYEAFVRRVAEVNPRLAERHARSIRSGEIEL
                     KLTADGAVEAVNTSERHARARLLVSGGAEAHTIELDLAPSESRVMPAPGGHLAFARLT
                     GWDGSTRDLIPHSESPAVLNTGVDELRDVPMVLEGTAPRPILVPEIASGQAFAIANDG
                     VTTTTETSLVLRAEAARFIHPEGSGGLFEVVEYDGLRLGRLAFALHQR"
BASE COUNT          191 a          392 c          374 g          190 t
ORIGIN      
        1 cgcgctgatg gagcgcgaac gccagacggc ccagccggag tccgtcgtac tcgaccacct
       61 cgaacaggcc ccctgatccc tcagggtgga tgaaccgcgc tgcctcagcg cgcagaacca
      121 gcgaggtctc cgtcgtcgtt gtcaccccgt cgttggcgat agcgaacgcc tgacccgaag
      181 cgatctcggg caccaggatc ggacggggag cagtgccctc gaggaccata ggcacatccc
      241 ggagctcgtc caccccggtg ttcagaactg ccggcgactc cgaatgcggg atcagatctc
      301 gggtcgatcc gtcccagccc gtcagcctgg cgaaggcgag atgccctcct ggagcgggca
      361 tgacccgact ctccgacggg gccagatcca gctcgatggt gtgcgcctcc gccccaccgc
      421 tgacgagcag gcgtgctcga gcgtggcgtt cggaggtgtt gactgcctcg acggcgccat
      481 cggcggtaag cttcaactcg atctcgcccg accggatgct gcgtgcgtgg cgctcggcca
      541 acctcgggtt cacctcggcg acccgccgga cgaatgcctc atagggctcg tctcgctcgt
      601 tgaccagccc gtagttgctg tcctcgggga aagtcgaact gatgcccagg gcgggctcgt
      661 cgacccacat gaagtagtgc tgcccgacca tgaagggcag atccgccacc atgttggcga
      721 agatctcgta gcaccgggcc ttctgttctt gggtatcgac ccgcatcccc gcccccgccg
      781 tgcacggcag cccactgtcg agtgccggga agctccactc ggtgatgatc atcggcttgc
      841 cggcatggtc gaagtagtca cagagctgct gaggcacgtc gagcacctgc ccgcggtcca
      901 tgtcgacata ggggtagctg ttcagtgtga agatgtcgtt gtgacgcccc gctgcctcga
      961 gcacctccgg cggggtgcgg ccagcgaacc ggcacccgat aaccatgtgg tcggggtcgg
     1021 cctcgcggag cgcgcggcat gcctcgccga agtacgcctc ggcgatcacg cgcaggaact
     1081 cggcacggac cgtgtccagc tcggcggacg gctcggggag ctctgtcgaa cgcaggaagg
     1141 cctcctc
//
LOCUS       DAOSPL010001571         1144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_126763_length_1144_cov_2.629936, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001571 DAOSPL010000000
VERSION     DAOSPL010001571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126763_length_1144_cov_2.629936"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLQ54_21485"
     CDS             complement(<1..189)
                     /locus_tag="PLQ54_21485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95895.1"
                     /translation="MSTVCYRQAELDLRQAKPGVAGLDGGDLTSDAGMLAQFGSPSWS
                     CSANASSAWPRAMRMSTTP"
     gene            521..835
                     /locus_tag="PLQ54_21490"
     CDS             521..835
                     /locus_tag="PLQ54_21490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95896.1"
                     /translation="MRPARVSALALPVVVAALTMPSWAAPDDLDALLDGVREIAAPGS
                     IPGPVCAFGPDAFCVIAGTSGRARLAVVGASRLGEGRVVAYGHEGLMGGAPDGATGVV
                     RT"
     assembly_gap    847..856
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <857..>1144
                     /locus_tag="PLQ54_21495"
     CDS             <857..>1144
                     /locus_tag="PLQ54_21495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95897.1"
                     /translation="GADGLRLLVNAIGWASGGGGVLGRVAICGLGGLVETVTGAGLAP
                     QRLAPGDLPAGLADVDVLIAHPGSIGADLVGAVLTFVRQGGGFITVVCPWGW"
BASE COUNT          146 a          362 c          428 g          198 t
ORIGIN      
        1 tggggtcgtt gacatcctca tagcccgcgg ccaggccgaa gatgcgttgg cggagcagct
       61 ccatgatggg gaaccgaact gcgcgagcat gcccgcgtcg gacgtcagat cgccgccgtc
      121 gagaccggcc accccaggct tggcttgccg gagatcgagc tcggcctgcc gatagcaaac
      181 ggtggacatg ggaagtcgac tccgctctcc gaagagctcg cacagaccca tggcaccagg
      241 ggatccagcg acttcactgc ctctgttcct ccacttgctc atgcatggtc tgggctagag
      301 gcgttccgag gggccgcaca ggggtgcgcc cgaagggcgc gttgggtgcc cccaggccaa
      361 aggcacgcac cgggcacatc ggtagcagaa ggcgcccggg acacgggtaa cactctgccc
      421 ctccagacgc ctcgggtgcg tccatggcac ggccgggcga ggtgccgtat gctggagggg
      481 tgccctggcg ccttggatcc tctggtggag gcgttccgtc atgagacccg ctcgcgtgtc
      541 cgcgttggct ctgcccgtcg tggtggcagc gcttacgatg ccctcctggg ctgcaccgga
      601 tgacctggac gccctgctcg atggcgtccg ggagattgcc gcacccggca gtatccccgg
      661 gcctgtgtgc gccttcggtc cggatgcttt ctgcgtcatc gcgggcacca gcgggcgggc
      721 ccggctggcc gtggtcgggg cgtctcggct cggcgagggg cgagtggtgg cgtatgggca
      781 cgagggcctg atgggcggag cgcccgatgg cgcgactggc gtggtgagaa cgtgaacggg
      841 tggcatnnnn nnnnnnggcg ccgacggcct gcgactcctg gtcaatgcga tcggttgggc
      901 ttcgggcggg ggtggtgttc tcgggcgggt ggcgatttgc ggcctcggtg gcctggtcga
      961 gacggtgact ggggccggac tcgcgcctca gcgcctggct cccggtgacc taccggccgg
     1021 cctggcggac gtcgatgtgc tcatcgcgca ccccggctcc atcggggcgg acctcgtggg
     1081 cgccgtcctg accttcgtcc gccagggcgg agggttcatc accgtcgtct gtccgtgggg
     1141 ttgg
//
LOCUS       DAOSPL010001572         1144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_126764_length_1144_cov_2.620753, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001572 DAOSPL010000000
VERSION     DAOSPL010001572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126764_length_1144_cov_2.620753"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1144)
                     /locus_tag="PLQ54_21500"
     CDS             complement(<1..>1144)
                     /locus_tag="PLQ54_21500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOS95898.1"
                     /translation="EEFGFATAPLVVMGCVMMRVCHMDTCPVGVATQNPELRGRFTGD
                     PQHVVHFMQFVAQELREIMAQLGFRTVDEMVGRVDRLEVRPAIEHWKAKGLDFSQILH
                     CPDVPPDWGRRCQMPQDHGLDKALDTTTLLPLCEPALERGEPVEATLPIRNVNRVVGT
                     LLGSEVTRRYGAEGLPDDTITLRFHGSAGQSFGAFIPRGITLVLEGDSNDYIGKGLSG
                     GKIVVRPPAGSTFVPEENTLIGNVALYGATRGEAYFRGMAGERFCVRNSGALAVVEGV
                     GDHGCEYMTGGRVVVLGATGRNFAAGMSGGIAYVLDPAGDFASRCNLEMVELCSLGDA
                     DERAFVEGMITRHAELTGSDLAWRILADWDTSAGRVVKVYPKDYRRA"
BASE COUNT          201 a          398 c          362 g          183 t
ORIGIN      
        1 gggcgcggcg gtagtccttg gggtagacct tcacaacccg gccggcactg gtatcccagt
       61 cggccaggat gcgccacgcc agatcgctgc ccgtcagctc cgcgtgccgc gtgatcatgc
      121 cctcgacgaa ggcccgctcg tcggcatcgc caagcgagca caactccacc atctccagat
      181 tgcaccgcga cgcgaagtcg cccgccgggt cgagtacgta agcgatgccg cccgacatcc
      241 cggccgcaaa gttgcggccg gtggcaccga gcacgaccac acgcccaccg gtcatgtact
      301 cacagccgtg gtcacccacg ccctcgacca ccgccagcgc gccgctgttg cgcacgcaga
      361 agcgctcgcc ggccatcccg cggaagtagg cttccccgcg ggtcgcgccg tacagggcca
      421 cattaccaat gagcgtgttc tcctctggga cgaacgtcga gcccgccggt ggccgcacca
      481 cgatcttgcc acccgatagg cccttgccta tgtagtcatt cgagtcgccc tcgagaacca
      541 gggtgatgcc tcgaggtatg aaggcgccaa agctctgacc ggccgatccg tgaaagcgca
      601 gcgtgatcgt gtcgtcgggg agtccctcgg ctccgtaccg acgtgtgacc tcgctaccca
      661 ggagggtccc cacgacacgg ttgacattgc ggatagggag tgtcgcctcg acgggctcgc
      721 cgcgctcgag cgcgggctcg caaagaggca gcagcgtggt ggtgtccagg gccttgtcca
      781 ggccatggtc ctgaggcatc tggcaacggc ggccccagtc tggtgggacg tcggggcagt
      841 gcaggatctg cgagaagtcg agtcccttgg ctttccagtg ttcgatggca gggcgcacct
      901 ctagccgatc gacccggcca accatctcgt cgaccgtgcg gaagcccaac tgggccatga
      961 tctcgcgcag ctcctgtgcc acgaactgca tgaagtgaac cacgtgctgg gggtcgcccg
     1021 tgaagcggcc gcgcagctcg gggttctgtg tcgccacgcc gacgggacag gtgtccatgt
     1081 ggcagacccg catcatgaca cagcccatca caacgagtgg agccgttgcg aacccgaact
     1141 cctc
//
LOCUS       DAOSPL010001573         1143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_126907_length_1143_cov_3.312500, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001573 DAOSPL010000000
VERSION     DAOSPL010001573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126907_length_1143_cov_3.312500"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            293..1051
                     /locus_tag="PLQ54_21505"
     CDS             293..1051
                     /locus_tag="PLQ54_21505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOS95899.1"
                     /translation="MIAGGSVAAQGRPMLVLVVDDSQLLRRLVTAAVEKGGHRVATAA
                     DGQEALNVLRNLPVDAIVTDILMPGMDGLTFARMVRLDPRTAHVPILVYSTAADRQLV
                     EEAIEAGVQDYLVKPANAQVLAQRLDRLEKQALAASDFHPVAVATHLGHGPDPKAGFR
                     ALADLAAEAQEALVPLAEALGSGRTAEARVIATRIHGAAAVLGVDSLAKPAGEVSRYL
                     AQGRLTAAIGRYGEIASVVSRLQRFLQSWPDAEG"
BASE COUNT          174 a          354 c          436 g          179 t
ORIGIN      
        1 cagggcacat gatggagtag tcgctgtggc cctgtcgggc gatggtaagc atggggcgag
       61 cctcctgagc accaagagcg gaagcagcca gcgcagtaca ggcgccggca cggaggaact
      121 ggcgccggtt cattgaggcg gccacggaca ctctcctgtc agtgtgggcc caggggccca
      181 tgtggggtgg cattcagcgc atgcgattgc aggtcctccg cgagggggct gccgcgtgtc
      241 gcgggcgcgc cggtcgcccg cctgcccgca cggaccacgg gggaggtgtc acatgatagc
      301 cgggggaagc gtggccgctc aggggcggcc gatgctggtg ctcgtggtgg atgacagcca
      361 gcttctgcgt cgtctggtaa cggctgccgt cgagaagggc ggccatcggg ttgccacggc
      421 cgccgacgga caagaggcgc tcaacgtgct gaggaatctg cccgtcgatg cgatcgtgac
      481 cgatatcctg atgccgggaa tggatggtct tacgtttgcg cgcatggttc ggctggaccc
      541 ccggaccgct catgtaccca tcctggtgta ctcaaccgcg gcggacaggc aactggtgga
      601 ggaggccatc gaggcgggag tccaggacta cctcgtgaag ccggccaacg cccaggtgct
      661 ggcccagaga ctggaccgac tcgagaagca ggcgctggcc gcgtcggact tccatcccgt
      721 ggccgtggcc acccacttgg gtcacgggcc cgacccgaag gccggcttcc gggccctggc
      781 tgatctggcg gcggaggcac aggaagcgct ggtgccgctc gccgaggcat tgggctccgg
      841 caggacggcc gaggcccggg tgatcgccac gcggatccac ggagccgccg cggtgttggg
      901 ggtggactcg ctggccaagc ccgcgggcga ggtgtcacgg tatctggctc aggggcggct
      961 cactgcggcc atcgggcgct atggtgagat cgccagcgtc gtgtcgcggc tgcagcgctt
     1021 ccttcagtcg tggcctgacg cggaggggta gatggccgac ccggtcgtta tccgcgccga
     1081 ccgagtggcg caggtgatcg cccgccatcc ctgggtcgat gccgggatgg tacggcggca
     1141 cac
//
LOCUS       DAOSPL010001574         1142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_127102_length_1142_cov_2.866605, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001574 DAOSPL010000000
VERSION     DAOSPL010001574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127102_length_1142_cov_2.866605"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLQ54_21510"
     CDS             <1..364
                     /locus_tag="PLQ54_21510"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOS95900.1"
                     /translation="VGAGFAVRNMVGSQTNDQMRSDGRTVLFDSNQAGGITGGLSTGQ
                     PIIVRLAVKPTPTIAKAQHTIDKYTLENRELAAITRRDPTIVARVWPVAEAFTALVIL
                     DNLMAYYGYEELKRRTGS"
     gene            complement(477..>1142)
                     /locus_tag="PLQ54_21515"
     CDS             complement(477..>1142)
                     /locus_tag="PLQ54_21515"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOS95901.1"
                     /translation="RRSWLTDLVGGNRRPLRGGPVYEFPLRPQQIVTLRLSTPTAVPE
                     VQPLRLWDELVPPHKLAVLRRWLRDRVGHPPLGPDLPVEDIPDLPEDAGSALNLGKPT
                     TASNVYRGMSVYGPEMASDGDAGTRWATDDTVSQAWLAVDLGRPERIGRAYLSEAYGR
                     VRAFELQMERGGDWVTFARGGPIGTNLDIEFEPVTAQRVRLLITDGPGGPTLWEFMLF
                     EPR"
BASE COUNT          205 a          382 c          375 g          180 t
ORIGIN      
        1 tgtgggcgcc ggattcgccg tcaggaacat ggtcggcagc cagacgaacg accagatgcg
       61 ctccgatggc cgaacggtgc tcttcgactc caatcaggct ggcgggatca cggggggcct
      121 aagcacagga cagcccatca tcgtgcgcct ggccgtcaag ccaacaccca caattgctaa
      181 ggcgcaacat acgattgaca agtacactct cgaaaatcgc gagctggcgg ccatcacccg
      241 gcgcgacccc accatcgtcg cgcgcgtctg gccggtcgcc gaggcgttca ccgccctcgt
      301 gatcctggac aatctgatgg cctattacgg atatgaggag ctgaagagaa ggactggcag
      361 ttaaccttcg gctaaccgac tatcagcttg ccatatcatg tgctgggtgg atccgtgggg
      421 ccatcccccg cctggccggc gcgggctgcg cgcggaacga tcgctgtggc actgcctcac
      481 ctcggctcaa acaacatgaa ctcccaaagg gtcggcccgc ccgggccatc ggtgatgagg
      541 aggcggacgc gctgggcggt gacgggctcg aactcaatgt ccagattcgt gccgatgggg
      601 ccgccccgtg cgaaggtcac ccaatcgccc cctcgctcca tttggagttc gaaagcacgc
      661 actcggccgt atgcctccga gagataggcc cggccgatgc gttcggggcg gccgaggtcc
      721 accgccaacc acgcctgcga aaccgtgtcg tccgttgccc agcgcgtgcc cgcatcaccg
      781 tcggacgcca tctcgggccc gtagactgac atgccgcggt agacgttcga cgcggtcgtg
      841 ggcttgccca ggttgagtgc gctcccggcg tcctcgggca ggtccggaat gtcctcgacc
      901 ggcaggtcag ggccgagtgg cggatgcccg acccggtcac gcagccatcg ccgcagaacc
      961 gccagcttgt gaggcgggac gagctcgtcc cacaggcgta gtggctggac ctcgggtacc
     1021 gcggtgggcg tgctcagacg cagggtcacg atctgctgcg ggcgcagcgg gaactcatag
     1081 accggcccac cacgcagggg acgccggttg cctcctacga ggtcggtgag ccaggaccgc
     1141 cg
//
LOCUS       DAOSPL010001575         1140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_127400_length_1140_cov_3.960369, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001575 DAOSPL010000000
VERSION     DAOSPL010001575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127400_length_1140_cov_3.960369"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="PLQ54_21520"
     CDS             <1..977
                     /locus_tag="PLQ54_21520"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOS95902.1"
                     /translation="PAGIREGQKIRLAGQGNGGDFYLNVRFLQHPEFRREGDDLFFVD
                     GEKEPRLRGTGTEDYFCDAWGFRQLDGPFYGIPLWEGYGTGDRGSAYRFHLADPVAFR
                     KSLRVEIEHKGSQNFPNGEGSGFIERDDLLSSVAYWYQTEPHKPWPALPKGADRLPVR
                     ESVLVEGESLLDVAQPSDQSPLELQGVGGTSGGKHLWYRPAGPGQSLEVTFALPKAIS
                     ADLRVLMVHSFDYGTYEAFLDGKSLGVWDLFSGTPISKEHQLGTVTLAAGEHTLRFEN
                     RGKNDESKGYLMGFDAIVSRQAVYSRPEEVDLRAIQVPRAPAYVRDRR"
BASE COUNT          183 a          388 c          377 g          192 t
ORIGIN      
        1 tccccgccgg catccgcgag ggccagaaga tccgcctcgc cggccagggc aacggcggcg
       61 acttctacct caacgtccgt ttcctccagc acccggaatt ccgccgcgag ggcgacgatc
      121 tcttcttcgt cgatggagag aaggagcccc ggctccgggg caccggcacc gaggactact
      181 tctgcgacgc ctggggcttc cgccagctcg atggaccctt ctatggcatc ccgctgtggg
      241 agggctacgg taccggagac cgcggcagcg cctaccgctt ccatctggcc gatcccgtgg
      301 cgttcaggaa gtcgctgcgg gtcgaaatcg aacacaaagg gtcgcagaac ttcccgaacg
      361 gcgagggcag cggcttcatc gagcgcgacg acctgctctc atcggtggcc tactggtatc
      421 agacggagcc ccacaagccc tggcccgcgc tgcccaaggg ggccgacaga cttccggtcc
      481 gcgaatctgt gcttgtggag ggggagtcac ttctggatgt ggcccagcct tcggatcagt
      541 cgccactgga gctgcagggc gtggggggga ccagcggggg caagcacctc tggtaccgtc
      601 cggcgggtcc cgggcagtcg ctcgaggtca ccttcgcgct gccgaaggcc atctccgcag
      661 acctgcgcgt gctgatggtc cattcgttcg actacggcac ctacgaggcg ttcctcgacg
      721 ggaagtcgct cggggtctgg gatctgttca gcggcacgcc gatctccaag gagcaccaac
      781 tcggcacggt cacgttggcg gccggagagc atacgctgcg cttcgagaac cgcggcaaga
      841 atgatgagtc gaaggggtac ctcatgggct tcgatgccat cgtgtcacgt caggccgtct
      901 acagccgtcc ggaggaagtc gacctgcggg cgatccaggt gcctcgcgcg cccgcctacg
      961 tccgcgaccg gcgctgagag gtcgcggttc gtgtgcccaa cccgcatatc acccgggacg
     1021 gcctatgcca cccgatgctg tccgttcggc ctcatcagct cgtcagcgcc gtctgcacgc
     1081 gcggcggcct gatctgcccg gagtggccct cgagcgaggc ccatgcgttc ctggccgcag
//
LOCUS       DAOSPL010001576         1140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_127452_length_1140_cov_2.364977, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001576 DAOSPL010000000
VERSION     DAOSPL010001576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127452_length_1140_cov_2.364977"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1140
                     /locus_tag="PLQ54_21525"
     CDS             <1..>1140
                     /locus_tag="PLQ54_21525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95903.1"
                     /translation="AAFVLADTPTVVRTQRATATVALVNDTAAGAPCHVRLWAVSPTN
                     ESRTLADESVTVLAWGRAALHGSFEPYETGRWRIRAETRLEGETARVDEDSVWVLSEA
                     DPATTRMPVTLLTPSASMEALLAATGIAWQPFDPETSRPEVIVVPHLLPDEGYPYVEL
                     AEAELLAARGCTVVYMAQDDAAVYGLPATLTPTLGADWGSHMLLPEHPLLDGLTDAPL
                     LSQDRGLADLAPRRILQWESSLPMEPVLPYLDIRPPGPRGTARGGLVWGAGLATERHG
                     AGQRIYCQLECESRPESPVVRRFVGHLLWWAERSLPLQLSTVSAEDQSAYQRRMAAAL
                     RARQEHHADVLISRRVRAAKDTVLDRAFEPEQALALGLRANQPVP"
BASE COUNT          162 a          391 c          422 g          165 t
ORIGIN      
        1 tagcggcctt cgtcttggcc gacacgccta ccgtggtgcg cacgcagcgg gccaccgcca
       61 cggtagcctt ggtgaacgat accgcagccg gcgcgccctg ccacgtgcgg ctctgggccg
      121 tctcaccgac gaacgagagc cggacgctgg cggacgagag cgtcacggtc ttggcgtggg
      181 ggcgggccgc gttgcatggc agcttcgagc cctacgagac cggacgctgg cgtatccggg
      241 cagagacgcg cctcgagggt gagacggcac gggtcgacga ggactcggtg tgggtgctgt
      301 ccgaggccga cccggcaacc acgcgcatgc ccgtgacgct gctgacaccc tcggcgagca
      361 tggaggcact gctcgcggcc acgggcatcg cttggcagcc tttcgacccc gagacttcgc
      421 ggcccgaggt gatcgtggtg ccccacctac tgcccgatga gggctacccg tacgtggaac
      481 tcgccgaggc cgagctgctg gccgccaggg ggtgcacggt cgtctacatg gcccaggacg
      541 acgcggctgt ctatgggctg ccggcgacac taacgcccac gctcggggcc gactggggct
      601 cgcatatgct gcttcccgag cacccgttgc tggatgggct caccgacgcg cccctgctat
      661 cgcaggaccg gggcctggcc gacctggcgc cccggcgcat cctccagtgg gagagctcgc
      721 tgccgatgga gcctgtgctg ccctacttgg atatccgccc cccggggccg cgcgggacgg
      781 cgaggggcgg gctcgtgtgg ggcgccggac tggccaccga acggcacggc gcggggcagc
      841 gaatctactg tcagctcgaa tgcgagagcc ggcccgagtc gcccgtggtg cgccgattcg
      901 tgggccatct cctctggtgg gcggagcgct cgcttccgct gcagctgtcg acggtgtcgg
      961 cggaggacca gagcgcctac cagaggcgga tggcggcggc gttgcgggcc cgtcaggagc
     1021 accatgcgga cgtgctgatc agccgccggg tccgagcggc caaggacacg gtgctcgacc
     1081 gtgccttcga acccgagcaa gcactggcac tgggcttgcg ggccaaccag ccggtgccgg
//
LOCUS       DAOSPL010001577         1138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_127749_length_1138_cov_3.276085, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001577 DAOSPL010000000
VERSION     DAOSPL010001577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127749_length_1138_cov_3.276085"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PLQ54_21530"
     CDS             complement(<1..584)
                     /locus_tag="PLQ54_21530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOS95904.1"
                     /translation="MYVQQFFTRGLAHSSYLLGDTKTCAIVDPRRDIDIYLEAASAMG
                     MTITHILETHLHADFVSGHMDLARVTGATIYVPKAGSGSFDSVAVGQGDSFQIGNLSV
                     EVRDTPGHTPEHISYVVADRSRGDQPVSVFCGDTLFVGDVGRPDLFPGQARDLASRLY
                     DSLHQQLLTLPAYCEVYPAHGAGSLCGRAMGAKRT"
     gene            852..>1138
                     /locus_tag="PLQ54_21535"
     CDS             852..>1138
                     /locus_tag="PLQ54_21535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95905.1"
                     /translation="MERRPILPLGIGLVLLGLVALLNPFGPMDEGLGNTRAESAETPE
                     FLRSGRGPVPLVAPIEPSAAPVTDGEGFRPAARPSKVKTLRPRPAPISTAA"
BASE COUNT          191 a          355 c          404 g          188 t
ORIGIN      
        1 gtgcgcttgg cgcccatggc acgaccacag agcgagccag caccgtgggc ggggtagacc
       61 tcgcagtaag cggggagcgt caggagctgc tggtgcaggc tgtcgtagag ccgggacgcc
      121 agatcgcgcg cctgccccgg aaacagatcg ggtcggccca catcgccaac gaaaagggta
      181 tcgccgcaga acactgacac gggctggtcg ccgcgggaac ggtcggccac cacgtatgag
      241 atgtgctccg gggtgtgccc cggggtgtca cgcacctcga cgctcagatt gccgatctgg
      301 aatgagtcac cctgccccac tgccacgctg tcgaaggatc cactgccggc cttggggacg
      361 tagatcgtgg caccggtgac ccgggccagg tccatgtggc cggagacgaa gtcggcatgc
      421 agatgggtct ccaggatgtg tgtgatggtc atgcccatgg ccgatgccgc ttcgaggtag
      481 atgtcgatat cgcgtcgagg gtcgacgatg gcgcacgtct tggtgtctcc gagcaggtat
      541 gagctgtgtg cgaggcccct cgtgaagaac tgctggacgt acacgcaatc gctcctttcc
      601 gctacccaac gctgtggcgc gccgttgatg cgggcagcat ccgaggcaag cagcccggat
      661 aggtgagcgc tcgacgcccg gattcgagtt cccctggctg ccgggcgaat cggctcatgg
      721 cccgtcggcc cgcaaactcg gtgccgcccg gaaagtggag ttgctgtgga gcggcgcaac
      781 cttctggtgg ggtcctcgtc tctgtgggcg tagggcgaac gcggaccctg cgcggaaggg
      841 tgacagcaac catggaacgc cgaccaattc tgcctctcgg catcgggctt gtgctcctag
      901 gacttgtggc tctgctgaac ccctttggcc ccatggacga agggctcggg aacacgcgag
      961 ccgagagcgc tgagacgccg gagttcctgc ggagtggccg cggaccggtg cccttggtgg
     1021 cccccattga accgagcgcg gcgccggtga ccgacggcga agggttccga cccgccgcac
     1081 ggccgtcgaa ggtgaagact ctgcggcctc gcccggcgcc gatctcgacg gcggcgtg
//
LOCUS       DAOSPL010001578         1137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_127962_length_1137_cov_2.631238, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001578 DAOSPL010000000
VERSION     DAOSPL010001578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127962_length_1137_cov_2.631238"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..>1137
                     /locus_tag="PLQ54_21540"
     CDS             190..>1137
                     /locus_tag="PLQ54_21540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95906.1"
                     /translation="MPPLVCTAVLMTGAHGLSELQALDKPLSPLSGAAALGDLIRGPG
                     TWDVQDGVLRVSAADEVYGRAFVGGHPWIDCIFEATVAVEQAGDVPWAGLRLIVRGDE
                     ATGNLTTIGLWCGDRTIVIEQSTGLKFETLKNSTQPAIAPFDFELGRPYRLCVIADRG
                     GLFCFVDGQYVVHATEPTFASRPCGLVGFYAAKAAGTFSDITVRPLRRSTSPFTLHPA
                     SPLDISAYSPTVLKVGDRYQMWHTNRGQSYAESADGVHWVQPAGPDPVKPLSPAGQWG
                     DQWEADADVLRYGDRYLMSNPACSSVVGGAWDGMGFQHSR"
BASE COUNT          176 a          394 c          389 g          178 t
ORIGIN      
        1 aggggcccat ggagcaggga ggaggttgga cgtccactga caccatctgc agatggacgc
       61 cgtgtcgtgt caccgatccg cgtggctcca cacccgacca acaggaccgt ccgcccggaa
      121 agccgaacac tgacgctcga ccgccgctcg gcgtggtagg gcgccggttc gggagtggat
      181 gcctcagtgt tgcccccttt ggtatgcacc gccgtcctaa tgaccggcgc acatggactc
      241 tcggagctgc aggcactcga caagcccctc tctccgctga gcggcgccgc cgctctcggg
      301 gatctgatcc gtggccccgg gacgtgggac gtccaggacg gcgtgctgcg cgtcagcgcc
      361 gccgacgagg tatacggacg cgcctttgta ggtggccacc cgtggatcga ctgcatcttc
      421 gaggcgacgg tcgccgtcga gcaggccgga gatgtgccct gggcagggct gcggctgatc
      481 gtccgcggcg acgaggccac gggcaacctg acgacgatcg gcctctggtg cggggaccgg
      541 accatcgtca ttgagcagtc gaccgggctg aagttcgaga ccctcaagaa cagcacccag
      601 cccgccatcg cgccgttcga cttcgagctc ggccggccct atcgcctctg tgtcatcgcc
      661 gaccgcggcg ggctgttctg cttcgtagac gggcagtacg tcgtccacgc caccgagccg
      721 acgttcgcct cgcggccgtg tggcctggtc ggcttctatg cagccaaagc cgccggcacg
      781 ttcagcgata tcacggtccg gccgctgagg cgctcgacgt cgcccttcac gctgcacccg
      841 gcaagtcccc tggacatcag cgcctactcg cccacggtgc tcaaggtggg cgaccgctac
      901 cagatgtggc acacgaaccg cggccagtcg tacgccgagt cggccgatgg ggtgcactgg
      961 gtgcagccgg cggggccaga cccggtgaag ccactaagcc ccgcgggcca gtggggcgac
     1021 cagtgggagg ccgacgccga cgtgctgcgc tatggcgacc gctaccttat gtcgaacccg
     1081 gcgtgctcca gcgtcgtcgg cggggcctgg gacggcatgg ggttccagca ctcgcgc
//
LOCUS       DAOSPL010001579         1135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_128264_length_1135_cov_3.071296, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001579 DAOSPL010000000
VERSION     DAOSPL010001579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128264_length_1135_cov_3.071296"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PLQ54_21545"
     CDS             complement(<1..515)
                     /locus_tag="PLQ54_21545"
                     /inference="COORDINATES: protein motif:HMM:NF012381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOS95907.1"
                     /translation="MKRRPAGYVCAECGARAVRWVGRCPACDSWNTMVAEEPAAKEER
                     GGPLRLGDPSSAVRLADAPVDDVARLQTGIGEFDRVLGGGLVEGGVVLLGGEPGAGKS
                     TLVLQAADRLSRAGHVVVYVSAEESAHQVRLRANRLGISGDGVLLVTDNGLPGAFARA
                     RTTGAACVVIDS"
     gene            complement(512..>1135)
                     /locus_tag="PLQ54_21550"
     CDS             complement(512..>1135)
                     /locus_tag="PLQ54_21550"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS95908.1"
                     /translation="PTTNARPDLYSMRPDGTEVVRLTDSQGRNVDFRNTVIIMTGNVG
                     AREIHRGRALGFGGATGEAALTQEEADRRHAVMSSKIMDEVKRVFRPEFLNRLDEIVI
                     FRSLSREQILAIVDLELKRIHEALANRHMTLQLSEAAKELLGREGYDPSLGARPLRRA
                     IQRRIEDPVSEAILLERFRDGDTIAGDVENGEIVFRRVESPVECEAS"
BASE COUNT          195 a          387 c          375 g          178 t
ORIGIN      
        1 gagtcaatca ccacgcaggc ggctccggta gtccgggcgc gggcgaacgc ccccggcagc
       61 ccattgtcgg tgacgaggag cacgccatca cccgagatgc caaggcggtt cgcgcggagg
      121 cggacctggt gggccgattc ctcggccgag acgtacacga ccacatgccc cgcacgagac
      181 aaccgatcgg ccgcctggag cacgagtgtg gactttccag cccccggttc gccgcccagc
      241 aggacgacgc cgccctcgac caggccgccg ccgagcacgc ggtcgaactc gccgataccg
      301 gtctggagtc gggccacgtc atcaacagga gcgtcggcga ggcgcacggc cgacgacggg
      361 tcaccgagac gaagaggccc gccgcgctcc tccttggcgg cgggctcctc ggctaccatg
      421 gtgttccacg aatcacaagc tgggcatcga ccgacccacc gcacggccct cgcgccacac
      481 tcggcgcaga catagccggc ggggcgtcgc ttcacgacgc ctcgcattcc accggcgatt
      541 ccacgcggcg gaacacgatc tccccgtttt ccacatcccc ggcgatggta tcaccatcgc
      601 ggaagcgttc gagcaggatt gcctcggaga cggggtcctc gatacggcgc tggatggcgc
      661 gccgcagagg gcgggcgccc aatgaggggt cgtagccctc gcggccgagt agctccttgg
      721 cggcttcgga tagctgaagt gtcatgtgcc gattggccag ggcctcgtgg attcgcttca
      781 gctcgaggtc gacgattgcc aggatctgct cgcgagacag tgatcggaag ataacgatct
      841 catcgagacg gttgaggaac tcaggccgga agacgcgctt gacctcatcc atgatcttgg
      901 agctcatgac cgcgtgccgg cgatccgctt cctcctgggt gagtgccgcc tcgcccgtgg
      961 cgccgccaaa accgagagcg cggccacgat gaatctctct ggcgccgacg ttgccggtca
     1021 tgatgatgac ggtgttccgg aagtcgacgt tgcggccctg cgaatcggtc aggcggacga
     1081 cctccgtccc gtccgggcgc atgctgtaga gatccggcct cgcattcgtg gtcgg
//
LOCUS       DAOSPL010001580         1135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_128345_length_1135_cov_2.021296, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001580 DAOSPL010000000
VERSION     DAOSPL010001580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128345_length_1135_cov_2.021296"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1135)
                     /locus_tag="PLQ54_21555"
     CDS             complement(<1..>1135)
                     /locus_tag="PLQ54_21555"
                     /inference="COORDINATES: protein motif:HMM:NF025181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HOS95909.1"
                     /translation="MLAVAAQQVVDDQKVLANTAAILDELDYAQSVKFARVRHRDQKS
                     FEILQPPYDRVFAVSPATMDGQRLGESGKDSFELLCRRVAAYVYVSIDSMETTEAEAR
                     ITDFGPAMSAPDGDGRMPFLYTVGLKTIEYPADGVHRAVTSRVMHRLLGEWTKPWARS
                     DLYHEDQNVLMQHLGLGGGMSYFLGEEGRLTRIEPDDQFEPQFSSVNDMAHRAASLEE
                     AIRIEVASGIDSFRADERGAVIQRLLEAEGNVMAWLDAASSDRLPGQKPAIDRTSGWI
                     RDFLVRKQKRCQAACEAQLLRFLEEVFWDFRRGPGYVLSLLEWAKDEAQAYTQARLRR
                     AIEAPLPPPNDEMHIRRAFEAREDSITRALLCFTGSAERIVSGE"
BASE COUNT          201 a          363 c          384 g          187 t
ORIGIN      
        1 ctcaccactc acaatgcgct cggcgctacc cgtgaagcac aacagggcgc gggtgatact
       61 atcctcgcgg gcctcgaatg cgcgacggat gtgcatttcg tcgttggggg ggggcagggg
      121 ggcctcgatg gcgcggcgca ggcgtgcctg cgtgtatgcc tgagcctcgt ccttcgccca
      181 ctcgagcagc gagagcacat agcccggccc acggcggaag tcccagaaga cctcctcgag
      241 gaaccgaagc agttgcgcct cacaggcggc ctggcaacgt ttctgcttgc gtaccaggaa
      301 gtcgcggatc cagcccgagg tacgatcgat ggccggcttc tggccgggga ggcggtcgct
      361 cgaggccgca tcgagccacg ccatgacatt gccctcggcc tcgagcaacc gctggatcac
      421 ggcaccgcgc tcgtcggcgc ggaaactgtc aatcccggaa gcgacctcga tgcgaatggc
      481 ctcctcgagg gaggcggcgc ggtgggccat gtcattgacc gaggagaact gcggctcgaa
      541 ctgatcgtcc ggctcgatcc gcgtgagccg accctcctcc ccgaggaagt aggacatccc
      601 gccaccaaga ccgaggtgtt gcatcagcac gttctgatcc tcgtggtata ggtccgagcg
      661 cgcccatggc ttggtccact cgccaagcag tcggtgcatg acacgggacg tgacggcgcg
      721 atggacacca tcggccgggt actcgatcgt cttgaggccc acggtgtaca ggaatggcat
      781 gcgcccatcg ccgtcagggg ccgacatggc ggggccaaag tcggtgatgc gtgcctcggc
      841 ctcggtggtc tccatggagt cgatgctcac atagacgtag gcggccaccc gacggcagag
      901 cagctcaaac gaatctttgc cgctctcgcc gagccgttgg ccgtccatgg tggccgggga
      961 cacggcgaag acccggtcgt agggcggctg gaggatctcg aaggacttct ggtcccggtg
     1021 gcgcacgcgc gcaaacttca ccgactgggc atagtcgagc tcatcgagga tggcggccgt
     1081 gttggcgagc accttctggt cgtcaaccac ctgctgggcg gcaacggcga gcatc
//
LOCUS       DAOSPL010001581         1134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_128504_length_1134_cov_2.110287, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001581 DAOSPL010000000
VERSION     DAOSPL010001581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128504_length_1134_cov_2.110287"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PLQ54_21560"
     CDS             complement(<1..854)
                     /locus_tag="PLQ54_21560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95910.1"
                     /translation="MDLSRLRERFVEYRTVVEPDRDTAEWWAGAPSVCRDDAGTFWMA
                     CRMREGDSPRGRRGYEIRLLRSDDGERFEPVHGIRREDVPIPGFERPALLFDPVTGRF
                     SLYACGPYDDGPWCVLRFDDAASPTEFVASTAQPVIAPEAPTANSRFNVVVGYKDPFI
                     LRGPDGLHCFAIGYDRAERTYHFRSLDGSSWEQVGGGPALDLGGWHTFYTRPACVLPL
                     GAGYLLVYEGSHPTWYDPVYNIATGLAWSADLRHFIDLTPDEPLLVSTTPGHYLTWRY
                     SHWMWAGGA"
BASE COUNT          184 a          393 c          380 g          177 t
ORIGIN      
        1 gcgccgccgg cccacatcca gtgcgagtag cgccaggtca ggtagtggcc cggggtggtg
       61 ctgacgagaa gcggttcatc gggagtcaga tcgatgaagt ggcgcagatc ggcgctccag
      121 gccaggcccg tggcgatgtt atagaccggg tcgtaccatg tgggatgcga gccctcgtac
      181 accagcagat agcccgctcc cagcgggagc acgcatgccg gtcgcgtgta gaacgtgtgc
      241 catccgccca gatcgagagc cgggccgcct cccacctgtt cccaggaaga cccgtccagt
      301 gagcggaagt ggtatgtccg ctccgcccgg tcatagccga tggcgaagca gtgaaggccg
      361 tcgggccccc gcaggatgaa tggatccttg tagccaacca ccacattgaa ccgcgagttg
      421 gcagtcggcg cttcgggggc gatgacgggc tgcgccgttg aggccacgaa ctccgtgggt
      481 gaggccgcgt cgtcaaaccg gagaacgcac cacggcccat cgtcataggg gccgcaggcg
      541 tagaggctga agcgccccgt gactgggtcg aacagcagcg ccggacgctc gaagcccggg
      601 atgggcacgt cttcccggcg gatgccgtgc accggctcga aacgctcacc gtcgtcgctg
      661 cgcaggaggc ggatctcgta gccacgccgg ccacgcggcg agtcgccctc cctcatgcgg
      721 cacgccatcc agaatgtgcc tgcgtcatcc cgacagaccg acggggctcc ggcccaccac
      781 tcggccgtgt cccgatcggg ctcaaccacc gtgcggtact cgacgaacct ctcacgcaag
      841 cgactcaggt ccatgtgatc gcgctccccc gctatccagg catcctccgc tgccgtttcc
      901 gtggggctct ccggtgggcc tccgaacgcg gcatcaaccg atggtcccca ccgtgccctt
      961 cgggtatgct ggcacctgag gaggacgctc ccatggcccc catcacgtgg cgcggttcgg
     1021 taatggctgc ggccctggcc ctcgccgcgg cggtgggcgc gcccgatgct cgggacccga
     1081 cccgcgtcgg cgtctactgg tgggatgcct ggtgggaggg ctccccttac atcc
//
LOCUS       DAOSPL010001582         1127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_129642_length_1127_cov_3.033582, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001582 DAOSPL010000000
VERSION     DAOSPL010001582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129642_length_1127_cov_3.033582"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="PLQ54_21565"
     CDS             complement(<1..1003)
                     /locus_tag="PLQ54_21565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95911.1"
                     /translation="MTTRCTRWMVFVLVAGTAGSTAASLEEGSAMARSDVARVRVEVA
                     PGAGPVEHRAAELFSRCVMDRTGKALARSSSASTRCTITIGRADAQLPRFVVDRDLRA
                     DGYRILAQPTHRGRVYVAGQSPSGVVAGVGALLRAMAFEPGAIRIPEMDLCDAPELPV
                     RGIYFATHFGNFYHVAPLEEVDRVIEEFALWGGNELVVWFDMHHFRDFADPAARAHVA
                     RLRHFADTAHAVGMRFGLTFIANEGYDSSPEALRADGNTGTAHYHRELCPSKPEGLAL
                     IGRWQAEVLDAFRDVDLAWTWPYDQGGCACPDCAPWGANGFLKASEQLARLYRARFPQ
                     "
BASE COUNT          184 a          408 c          376 g          159 t
ORIGIN      
        1 cctgagggaa gcgcgcgcgg tatagacgcg ccagttgctc ggaggccttg aggaagccgt
       61 tggcgcccca cggcgcgcag tccgggcagg cgcacccgcc ctggtcgtac ggccacgtcc
      121 aggccagatc gacatcgcgg aacgcatcca gcacctcagc ctgccaccga ccgatgagcg
      181 cgaggccctc gggcttcgag gggcacagct ctcggtggta atgggccgtg ccggtgttgc
      241 cgtcggcgcg cagggcctct gggctggagt cgtagccctc gttcgcgatg aaggtgagac
      301 cgaagcgcat gccaacggcg tgagcggtgt cggcgaagtg ccggagccgc gccacatgag
      361 cccgagccgc cgggtcggcg aagtcacgga agtggtgcat gtcgaaccag accaccagct
      421 cgttgccacc ccagagcgcg aactcctcga tgacgcgatc gacctcctcc agcggcgcga
      481 cgtggtagaa gttcccaaag tgcgtcgcga agtagatgcc ccgcacgggg agctccggtg
      541 cgtcacacag gtccatctcg ggaatccgga tggcccccgg ctcgaacgcc atggcccgaa
      601 gcaaggctcc cacgccagcc accacgccgc tggggctctg gccggcaacg tagacgcgcc
      661 cacggtgggt gggctgggcg aggatgcggt agccgtccgc ccgcaggtcc cggtcgacga
      721 cgaaccgggg gagctgcgcg tccgctcgac caatagtgat cgtgcaccgc gtcgaggcac
      781 tcgaagatct cgccagggcc ttccccgtcc ggtccatcac gcaccgggag aacagctcgg
      841 ccgccctgtg ctcaaccggc ccggcaccgg gggccacctc gacacggacc cgcgccacgt
      901 cggaacgcgc catggcgctc ccctcttcca ggctcgcggc agtgctcccg gcggtaccgg
      961 ccaccagcac gaacaccatc catcgcgtgc atctcgtcgt cactcgcacg accagctcct
     1021 ctgctctggg caacccattc cgtgtgcggc cggaggtacg ttatcacccg gatcccatcg
     1081 gcaccggtgg cgacgctcgc gcggccagtt ggcgcccttc gcttgtg
//
LOCUS       DAOSPL010001583         1123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_130321_length_1123_cov_3.594569, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001583 DAOSPL010000000
VERSION     DAOSPL010001583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130321_length_1123_cov_3.594569"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PLQ54_21570"
     CDS             <1..527
                     /locus_tag="PLQ54_21570"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS95912.1"
                     /translation="DYLWLGQGYVAGIPVPADVAAIATAGVAFVLRWTPFGRSLYALG
                     NNPSAAEHQGVSRRGTEWRVYVLAGAMVGLSALTFAPCFTSIQTNTGLGFEMIVITAV
                     VVGGTNIMGGRGTVLGTVLGVMLLAAIGNGLDLLGSTEMDYWESAFQGALVLLAVMTD
                     YIQSRRRRQGAGIE"
     gene            524..>1123
                     /locus_tag="PLQ54_21575"
     CDS             524..>1123
                     /locus_tag="PLQ54_21575"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOS95913.1"
                     /translation="MSQSSAPSRHLSAERVLLVLFLAVVGTMCAVSPYFRIAQNLLET
                     MRYLVEPGIVALAMTLVIITGGIDLSVGAIVGLSAVLLGMAWKSWGLPVGAAVGVAIG
                     AGALCGLINGVMVVAFRIPPLIVTLATMAVFRGLATGLCHGTPVSGFPESLRPLGQGY
                     YAIGGGLRLPAQTLILVGLAVAAWVFLSRTRHGRCIYAIG"
BASE COUNT          133 a          363 c          415 g          212 t
ORIGIN      
        1 ccgactacct ttggctgggc cagggctacg tggcgggtat cccggtgccg gccgacgtgg
       61 cggccatcgc cacggcgggg gtcgccttcg tcctgcggtg gacacctttc ggccggagcc
      121 tctacgcact cggtaacaat ccatcggccg ccgagcacca gggcgtgtcg cgccggggga
      181 cggagtggcg ggtctatgtg ctggcgggcg ccatggtggg gctatccgcg ctgaccttcg
      241 cgccatgttt cacctccatt cagacgaaca cgggcctcgg tttcgagatg atcgtgatca
      301 cggccgtggt ggttggcggc acgaacatca tgggcggccg cgggacggtc ctcggcacgg
      361 tgctgggcgt gatgctcctg gccgccatcg gcaacgggct cgacttgctt gggtcgacgg
      421 agatggatta ctgggagagc gccttccagg gcgcgctggt gctgctggcg gtcatgaccg
      481 actacatcca gtcgcgccgc cggcgacagg gggccgggat cgaatgagtc agtcctcggc
      541 cccgtcccgc cacctgagtg ccgagcgcgt gctgctggtg ctattcctgg ccgtcgtcgg
      601 cacgatgtgc gcggtctcac cttacttccg catcgcgcag aatctgctgg agacgatgcg
      661 gtacctggtc gagcccggca tcgtggcgct ggccatgacc ctggtgatca tcacgggggg
      721 catcgacctc tcggtcggcg ccatcgtggg cctctcggcc gtgctccttg gcatggcgtg
      781 gaagtcgtgg ggcctgccgg ttggggcggc cgtgggggtc gccatcggcg ccggggcgct
      841 ctgcggcctg atcaacggcg tcatggtggt tgcgttccga atcccgccgc tgatcgtgac
      901 tctggccacc atggcggtgt tccgcggtct ggcgacgggc ctctgccacg gcacgccggt
      961 gagtggtttc cccgagtcgc tgaggcccct ggggcagggg tactacgcca tcggtggcgg
     1021 cctccggctc cccgcgcaga cgctgatcct cgtggggctg gcggtcgccg catgggtgtt
     1081 cctcagtcgg acccggcacg ggcggtgcat ctacgccatc ggg
//
LOCUS       DAOSPL010001584         1123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_130331_length_1123_cov_3.220037, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001584 DAOSPL010000000
VERSION     DAOSPL010001584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130331_length_1123_cov_3.220037"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PLQ54_21580"
     CDS             complement(<1..1115)
                     /locus_tag="PLQ54_21580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95914.1"
                     /translation="MRIQQGPGDGLCRFVTSIQISGHQTEWRKVSDRWPLVSQPAAWT
                     AIPLRPCVCRYVKVAFASGSREDLAEVASVEVLGAPQERLRIFALYWGDLPGLAACAG
                     DVASLGVSDLLVSWVDGPAVCSWHQGLDAFVDSGAKAALDRAGVRYWLDEHELCSIIR
                     TAADIGDDLIWDQACARARQCYARAKELGFSGASLDPEHYYGDDRAWGDVAQYGLAGL
                     YYRRGQQLGQAMAEVWPGIRTVHIYEARIYGNFRGVGDPSDPNMDYAQGHMWWLKGMK
                     DAGVGITLGLEKTYGAGEGEVLDALPEAQRVKVQGTGHLFRWFDHTPDWYLNLAWQAY
                     PFVDEIIPGFNPWSVSEGDLSGVHYRPEYLREQLEYAR"
BASE COUNT          189 a          421 c          336 g          177 t
ORIGIN      
        1 cgcgcatact cgagctgctc ccgcaggtac tcgggccggt agtgcacgcc cgagaggtct
       61 ccctcgctca ccgaccacgg gttgaagcca gggatgatct catcgacgaa cggataagcc
      121 tgccatgcca ggttgaggta ccagtcgggc gtgtggtcga accagcgaaa caagtgtccc
      181 gtgccctgca ccttgactcg ctgggcctcg ggcaacgcgt cgagcacctc cccctcgccg
      241 gcgccatagg tcttctcgag ccccagtgtg atgccgacac ccgcatcctt catccccttc
      301 agccaccaca tgtgcccctg ggcgtagtcc atgtttggat cgctggggtc gcctacccct
      361 cggaagttgc cgtagatccg cgcttcgtag atgtgcacgg tccgaatgcc cggccacacc
      421 tcggccatgg cctgtccgag ctgctgccca cgccggtagt agagcccggc caggccgtac
      481 tgcgccacat cgccccatgc gcgatcgtcg ccgtagtagt gctccgggtc gagactcgcg
      541 ccggagaatc ccagttcctt cgcccgagcg tagcactgcc gcgcgcgcgc acaggcttgg
      601 tcccagatga ggtcatcgcc gatgtcggcg gccgtccgga tgatcgagca cagctcgtgc
      661 tcatcgagcc agtagcgcac gccggcgcga tccagtgccg ccttggcgcc cgagtcgacg
      721 aaggcatcca gcccctggtg ccaactgcac acggccgggc cgtcgaccca cgagacgagc
      781 agatcgctca cgcccagcga cgccacatcg cccgcacatg ccgccagtcc cgggagatcg
      841 ccccagtaca gggcgaagat ccgcaaccgc tcctgcggtg cgccgagcac ctccaccgag
      901 gccacctccg ccaagtcctc ccggctccca gaggcgaacg ccaccttcac atagcgacag
      961 acgcaggggc gcagtgggat ggccgtccac gcggccggct ggctcaccag cggccatcgg
     1021 tctgacacct tccgccactc cgtctgatgg ccgctgatct ggatggaggt gacgaaccgg
     1081 cacaggccgt cacctgggcc ctgctggatc cgcacctcct gca
//
LOCUS       DAOSPL010001585         1121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_130720_length_1121_cov_2.738274, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001585 DAOSPL010000000
VERSION     DAOSPL010001585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130720_length_1121_cov_2.738274"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1121
                     /locus_tag="PLQ54_21585"
     CDS             <1..>1121
                     /locus_tag="PLQ54_21585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOS95915.1"
                     /translation="HRIRAKRTDGGTETCVAPVDPELADKLCQLNPNVTLYVEGTRTA
                     EVWVQVLSSLIIPVGLFVFIVIMFRQMQAGSGQALSFGRSRAKRWTDSFDHVTFEDVA
                     GVEEAKEELQEVVEFLKEPEKFRAMGARIPRGIILTGSPGCGKTLLARAIAGEANVPF
                     YYISGSDFVEMFVGVGASRVRDLFEQAKANERCIVFIDEIDAVGRHRGTGLGGGHDER
                     EQTLNQLLVEMDGFDKNSGVIILAATNRPDVLDPALLRPGRFDRRIHVDNPSLGGREG
                     IFEIYTRDRPISPDVDLRALAQRTPGFTGADIEILVNEAAILAARRNKKEIDRSDFED
                     ATDRVQVGLERRSKILSEGERRALAYHEAGHALMGLLLPG"
BASE COUNT          200 a          331 c          398 g          192 t
ORIGIN      
        1 caccgcatcc gggccaaacg gaccgatggg ggaacggaga cctgtgtggc accggtggac
       61 cccgagctgg cggacaagct gtgccagctc aaccccaacg tgacgctgta cgtggaaggc
      121 acacgcacgg ctgaagtgtg ggtccaggtt ctgagctcac tgattatccc cgtcgggctg
      181 ttcgtcttca tcgtgatcat gttccgccag atgcaggcgg gcagcggtca ggcgctatcg
      241 tttggtcgga gccgggccaa gcggtggacc gacagcttcg atcacgtgac gttcgaggat
      301 gtggccggcg tcgaggaggc caaggaggag ctccaggagg tggtggagtt cctcaaagaa
      361 ccggagaagt tccgggccat gggggcgagg attccgcggg ggatcatcct cacgggatcg
      421 cccgggtgcg gcaagacgct attggcgcgg gccatcgcgg gagaggcgaa cgtacctttc
      481 tactacatca gcggatctga cttcgtcgag atgttcgtgg gcgtgggcgc ctcgcgggtg
      541 cgcgacctct tcgagcaggc gaaggcgaat gagcgctgca ttgtgttcat cgacgagatc
      601 gacgccgtgg gccgccaccg gggaaccggt ctgggtggag ggcatgatga gcgtgagcag
      661 acgctgaacc agttgctcgt cgagatggac gggtttgaca agaactcggg cgtcatcatc
      721 ctggcggcca ccaaccgccc ggatgtcctc gatccggcgc tactccgtcc cggccgtttc
      781 gaccgccgca tccatgtgga caacccgagc ctgggcggtc gcgagggcat cttcgagatc
      841 tacacccgtg atcgcccgat ctcgcccgac gtcgatctgc gcgcattggc ccaacgcaca
      901 ccgggcttca cgggtgccga catcgagata ctggtcaacg aggcggcgat cctggcggcg
      961 cgccgcaaca agaaggagat tgaccggagc gactttgagg atgccacgga ccgcgtccag
     1021 gtgggcctgg agcggcgctc caagattctg tcggagggtg agcggcgcgc cttggcgtac
     1081 catgaggcgg ggcacgcgct tatgggcctg ctcctgcccg g
//
LOCUS       DAOSPL010001586         1121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_130750_length_1121_cov_2.377111, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001586 DAOSPL010000000
VERSION     DAOSPL010001586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130750_length_1121_cov_2.377111"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PLQ54_21590"
     CDS             <1..402
                     /locus_tag="PLQ54_21590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95916.1"
                     /translation="VRDGDRPVKLHAFWDDAVGLTERTMRLLGRAGKMAADPELARAK
                     LPELERDTTPEAWALESRQVAADSVYLRGELSGARAESYRDADGRLRSRPPADAPPLP
                     PGYQDEARRVSLRRLALAAFRLEDELRGLGG"
     gene            613..1098
                     /locus_tag="PLQ54_21595"
     CDS             613..1098
                     /locus_tag="PLQ54_21595"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOS95917.1"
                     /translation="MYHVTTVTDGRRPLLRTDQARAIFARCLADCETQGLFSCLAYVL
                     MPDHLHMVIWLPHGHAVADGVRRLKGRFARELNVALGREGRVWQPSYWERTVRDEQAL
                     REMADYTHANPVRAGIVERPEDYPASSCGEFAERYVRTFGQPPPPSACSSATRRQCVR
                     G"
BASE COUNT          176 a          346 c          416 g          183 t
ORIGIN      
        1 gttcgcgatg gcgatcggcc cgtgaaactc cacgcgttct gggatgatgc cgtgggcttg
       61 acggaacgta ccatgcggct tctggggcgc gccggcaaga tggcggcgga cccggagctg
      121 gcgcgggcga agctgcccga gctcgagcgc gacacgacac ccgaagcctg ggcgctcgag
      181 agccgccagg tggcagccga ttccgtctat ctcaggggtg agctcagtgg ggcgcgggcg
      241 gagagctatc gggatgccga cggccgccta cggtcgcgtc cgccggcgga tgcaccgccg
      301 ctccccccgg gctaccagga cgaagcgcgg cgcgtgtcgc tccgccggct ggcgctggcg
      361 gccttccggc tcgaggatga gcttagaggg ttgggtggat aggcggagca cctctccatt
      421 tggggagtga ggccggcgtt cgcggtgctc actgtagccg aggcttgagc ctcggtacgg
      481 agggcctcgg gtgacaatgg gtgcgcgcga ggctgaagcc tcgcacataa gtgatcgaga
      541 ggtctggaaa tggccgcgcc accgaacggc ggtaggaatc cgttacggtc gagtctgttc
      601 cgagggacag gcgtgtacca tgtgaccacc gtaacggatg gccggaggcc gcttctccga
      661 actgaccagg cacgggcgat cttcgctcgg tgtctcgcgg actgcgagac ccaaggcctg
      721 ttcagctgcc tggcctatgt gctcatgccg gaccacctcc atatggtcat ctggttgccg
      781 cacgggcacg ctgtggcgga cggtgtccgt cggctcaaag gcaggttcgc gcgcgagctt
      841 aacgtagcgc tcggacgcga aggccgggtg tggcaaccct cctactggga gcgcaccgtt
      901 cgtgatgagc aggcactgcg cgagatggcc gactacaccc acgcgaatcc cgtccgcgct
      961 gggatcgtgg agcggcccga ggactacccg gcgtcatcgt gtggggagtt cgctgagcgc
     1021 tacgtgcgca cgttcgggca gccgccgccc ccgagtgcgt gctcttcggc cacacggagg
     1081 cagtgcgtgc gaggctgaag cctcgcctac gggaagcggg a
//
LOCUS       DAOSPL010001587         1119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_131170_length_1119_cov_1.951128, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001587 DAOSPL010000000
VERSION     DAOSPL010001587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131170_length_1119_cov_1.951128"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PLQ54_21600"
     CDS             <1..722
                     /locus_tag="PLQ54_21600"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOS95918.1"
                     /translation="GTYHSSLQVAALAGEVAEQLAMNDLLVRVGAMYHDIGKLRYPHF
                     FAENQFGGPNPHASLTPSMSALVLISHVKEGLERGRAAKLPVQILDFIGQHHGTTLIE
                     YFYHQACDAEGAENVPEERFRYPGPKPQSRETAIVMLADSVEAAVRSMREPTHSKIDS
                     MVRGIVRSRVEQGQLSESPLTLRDIDTAIDTLVRALQGIHHTRVEYPGDAELLQSAQT
                     AAGVAAPGGARPGRARANEHP"
     gene            719..>1119
                     /gene="ybeY"
                     /locus_tag="PLQ54_21605"
     CDS             719..>1119
                     /gene="ybeY"
                     /locus_tag="PLQ54_21605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOS95919.1"
                     /translation="MNLRVEVVIRNEQPCPVDESAIERVVCLTLGHAAGERAAECEVS
                     LLLVEPDAIRALNARHRAIDMPTDVLSFPQLDPFTVAPGEILGDVVVCPEITNRQADA
                     AGRRREDELLEVVAHGVLHLVGYDDGTPEGY"
BASE COUNT          201 a          379 c          365 g          174 t
ORIGIN      
        1 cgggaaccta tcactcatca ctccaggtcg cggcgctggc cggcgaggtt gcggagcagc
       61 tggcgatgaa cgatctcctc gtccgagtcg gcgccatgta ccacgacatc gggaagctcc
      121 ggtacccgca tttcttcgcg gaaaaccagt tcggcgggcc caacccccac gcgagcctga
      181 ccccgtcgat gtccgctctc gtcctcatat cccacgtgaa ggaggggctg gaacgcggtc
      241 gggcggccaa gttgccggtg cagatcctgg atttcatcgg ccagcatcac gggacaaccc
      301 tgatcgagta cttctatcat caggcttgcg atgccgaagg ggccgagaac gttcccgagg
      361 agcgattccg ctatcccggg cccaagcctc agtcgcgcga gaccgccatc gtgatgctgg
      421 ccgactcagt cgaggcggcg gtgaggtcca tgcgtgagcc gacccacagc aagatcgaca
      481 gcatggtgcg cgggatcgtg cggtcgcgag tcgagcaggg ccagctgagt gaatcgccgc
      541 tcacacttcg cgacatcgat acggctatcg acaccttggt gcgggcactt cagggcatcc
      601 accacacaag ggtcgagtac ccgggcgacg ccgagctcct tcagtcggcc cagacggcgg
      661 cgggcgtcgc cgcgcctggg ggcgcccggc ccggacgcgc gcgggcgaac gaacacccat
      721 gaatctgcgg gtcgaagtcg taatacgcaa cgaacaaccc tgccccgtcg atgagtcggc
      781 catcgagcgc gtggtatgcc tcaccctcgg ccacgccgcc ggcgagcgcg ccgcggagtg
      841 cgaggtcagc ctgctcctgg tcgagcccga cgccatccgg gcgctcaacg cgaggcaccg
      901 ggccatcgat atgccgacag atgtcctctc attcccgcaa cttgacccct tcacggtagc
      961 gcccggcgag attctgggtg acgtagttgt gtgcccggag ataacgaacc gccaggccga
     1021 tgcggcgggt cgccgccgcg aggatgagct cctcgaggtg gtcgcccacg gcgtactcca
     1081 tctggtgggc tacgacgacg gcacgcccga gggctacga
//
LOCUS       DAOSPL010001588         1118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_131258_length_1118_cov_3.381938, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001588 DAOSPL010000000
VERSION     DAOSPL010001588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131258_length_1118_cov_3.381938"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1118)
                     /locus_tag="PLQ54_21610"
     CDS             complement(<1..>1118)
                     /locus_tag="PLQ54_21610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95920.1"
                     /translation="GLADNGIATTTLVNHLTGSTFVFAAAHELALTLSSAIDGVAEPL
                     RFVDDFRVVDAAQPTPDSAEVRVRSPSTALEAALHYRLDGPTRRKWATVRNTGDQPLL
                     LLDVRLDDLATGARVSGGGRGWPLFIADQAFAVIEHPSGENLAAGGRVTLTHYPGKLL
                     APGEACETRVALISMAGANQAEAHFVDYLDAHAVRKPRVVLVYTPFGINNQWGPCPTL
                     TDEETLDVLGVLERWRQRGARFDYFTLDTGWPDPSSDMTRFRPWAYPDGPGAIVERVH
                     QLGMEFGLWFATSWAAESCWANRAAWGPQTQPTMRYRNGAPDKAGFGHVFCYGSEAYS
                     QLVRDAVLHHVRENGVRFVKFDGGSYACDDTTHGHLPG"
BASE COUNT          167 a          362 c          414 g          175 t
ORIGIN      
        1 tccccggcag gtgcccgtga gtcgtgtcat cacaggcgta gctgccgcca tcgaacttca
       61 cgaagcgtac gccgttctcg cggacatggt gcagcaccgc gtcgcgcaca agctgcgagt
      121 aggcctcgct gccgtagcag aacacgtgtc cgaagccggc cttgtccggc gcgccgttcc
      181 tgtagcgcat cgtgggctgg gtctgtgggc cccaggcggc gcggttggcc cagcaggact
      241 cggcggccca gcttgtcgcg aaccaaaggc cgaactccat cccgagctgg tgcacccgct
      301 caacgatggc tccggggccg tcaggatagg cccacgggcg gaagcgggtc atgtcggagc
      361 tcggatccgg ccagccggta tccagcgtga agtagtcgaa ccgcgcgccg cgctggcgcc
      421 agcgttcgag gacgccgagc acgtcgaggg tctcctcgtc ggtcagcgtc gggcacggcc
      481 cccactggtt gttgatgccg aagggcgtgt agaccaagac gacacgcggc ttccgcacgg
      541 cgtgggcatc caggtagtcg acgaagtggg cttcggcctg gttcgcaccg gccatgctga
      601 tgagggccac gcgcgtctcg caggcctcgc ctggagcgag gagcttgccg gggtagtgag
      661 tgagggtgac acgcccgccg gccgccaggt tctcgcccga cggatgctcg atgacggcga
      721 aggcttgatc ggctatgaag agcggccagc ccctgccgcc gcccgagact ctcgcgcccg
      781 tggcgaggtc gtcgagccgg acgtcgagca gcaggagggg ctggtcgccc gtgttccgca
      841 ccgtggccca tttccggcga gttggcccgt cgagccggta gtgcagcgcg gcctcgagcg
      901 cggtgctcgg gctccggacg cggacttcgg cgctgtcggg agtgggttga gccgcgtcga
      961 cgacgcggaa gtcatcgacg aagcggaggg gctccgccac gccgtcgatc gcggaggaca
     1021 gcgtcagtgc cagctcgtgg gcggcggcga atacgaacgt gctgccggtg agatggttca
     1081 ccagcgtcgt tgtggcgatg ccattgtcgg cgagcccg
//
LOCUS       DAOSPL010001589         1116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_131636_length_1116_cov_2.872762, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001589 DAOSPL010000000
VERSION     DAOSPL010001589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131636_length_1116_cov_2.872762"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..956
                     /locus_tag="PLQ54_21615"
     CDS             324..956
                     /locus_tag="PLQ54_21615"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOS95921.1"
                     /translation="MNRRRFCLAGCLFACLVPLPSPSWCAPRRPTGWRPLMDGYTFAG
                     WHMVGDGLWTVESGSLVGRACNEKLYGLLVSKDTFRDFRLRLRYKSDNGDSGVYIRTD
                     IQPPDEAHGLQIQVGPIGSGNGGIYESYDRGWLVHPTVDDEKRWVRPGDWNDLEILAS
                     GGTVEVRLNGTVTAQLRDDPGRPRGHIALQMHSGTVMDVRFRDIQVWENP"
BASE COUNT          175 a          334 c          420 g          187 t
ORIGIN      
        1 agagaaggcc cgttccgccc actcgcggtt gagcgcgacc tcaggtggca gatccaccgg
       61 agcggcgtgg gtgggcacga gcacgcctcc gataatgggc aggggcagga gagtgggcag
      121 gactcgcacg gcggagcctc cttgagcggg aacagccagt ggtgatgcgg gtactgtgtt
      181 cagcgcggcg gcccggccga ccttgggggc gcgtgggcca gccattcgcc ggcatgcaag
      241 ggcttgcgca tccccagggg gaaagccacg gtggccggca gcgtttcaac cggccggcag
      301 gtcggatcac ctgggagggt acgatgaatc gcagacgatt ctgtctcgct ggctgcctgt
      361 ttgcttgcct cgttccgctg ccctcgccgt cgtggtgcgc tccgcgccgg ccgactggtt
      421 ggcgcccact gatggacggc tacactttcg ccgggtggca tatggtgggg gacgggctct
      481 ggaccgtcga gagcggctcg ctcgttggtc gcgcctgcaa tgagaagctc tatggtctgc
      541 tcgtcagcaa ggacacgttc cgcgacttcc ggctgcgcct ccggtacaag tccgacaacg
      601 gcgatagcgg tgtgtacatc cgcaccgaca tccagccacc ggatgaggcg cacggtctgc
      661 agatacaggt ggggcctatc ggcagcggca acggcgggat ctatgagagt tacgaccgcg
      721 gatggctcgt gcaccccacg gtcgacgacg agaagcgatg ggtgaggccg ggcgactgga
      781 acgacttgga gatccttgcc tcgggtggta ccgtcgaggt gcggctcaac ggcaccgtga
      841 cggcgcagct ccgcgacgac ccgggccggc ccaggggcca catcgcgctc cagatgcact
      901 cgggcacggt catggacgtg cgcttccgcg acattcaggt gtgggagaac ccgtgaggac
      961 aagattcgga accggatgcc ggcggtcggt cgtgcttggc atcgcgtctc tgaccatcgg
     1021 ggtgtcggcg gtttcggcgc agcccgagca ggtggtgctg gcagggccgg gtggcacgcg
     1081 tgtggtgttc gcgaaggcgg gcgggcggta tcgctg
//
LOCUS       DAOSPL010001590         1114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_131990_length_1114_cov_3.440038, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001590 DAOSPL010000000
VERSION     DAOSPL010001590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131990_length_1114_cov_3.440038"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PLQ54_21620"
     CDS             <1..523
                     /locus_tag="PLQ54_21620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95922.1"
                     /translation="RDADWDLHPNASGVEISASCDLGAGRRGPQLTSTVYHDYATATL
                     NAFPLSEGGTVRLWLRHSGRLQLDLGRPLLQFDGSTWSIVEDPWREGHVYSIVTSGHV
                     ARELDVAVRGGYLDVVFGGQALARHVPVPEGVGAALLDVKTWGGDSLAFDVIEISTEP
                     MNLFEPQVDHPVR"
     gene            complement(529..>1114)
                     /locus_tag="PLQ54_21625"
     CDS             complement(529..>1114)
                     /locus_tag="PLQ54_21625"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOS95923.1"
                     /translation="APEETPSPQAMVQRFRSEFEALGGRFFWEPGSETATDRLSKLLR
                     DGELRRVACFDVPWLTSLVRGAGVEPFEKDLSVCDVCITTAHMLVAEQGLIVMAASPW
                     GPRAASSLSPVHIVVAQFAQLVWGLGDALARIGEVIEDGEMPSDIVFIAGPSRTADIE
                     KTLVIPAHGPRELCVVLIGEAGPPAEAGAGEEPA"
BASE COUNT          191 a          386 c          371 g          166 t
ORIGIN      
        1 ccgcgatgcg gactgggacc tccaccccaa tgcgagcggc gtggagatca gcgcaagttg
       61 cgacctcggc gccggacggc gcggcccgca gctcaccagc acggtctacc acgattacgc
      121 cacggcgacg ctgaatgcgt tcccgctgag cgagggcggc accgttcggc tctggctgcg
      181 ccacagcgga cggctacaac tcgatctcgg tcgcccgcta cttcagttcg acggcagcac
      241 ctggagcatc gtcgaggacc catggcgcga ggggcacgtc tactcgatcg tcacatcggg
      301 ccatgtggcg agggagctgg acgtcgccgt gcgcggcggc tacctggatg tggtctttgg
      361 cggccaggcc ctggcccggc acgttccggt gcccgagggg gtaggggcag cactactcga
      421 cgtgaagacc tggggcggcg actcgctcgc cttcgacgtg atcgagatct ccaccgaacc
      481 gatgaacctg ttcgagccgc aggtggacca tccggtgcgg taggagggct aggccggctc
      541 ctcacccgct ccggcctcag ccggcggccc cgcctcgcca atgagcacga cgcacagctc
      601 gcgggggccg tgagcgggga tgacgagggt cttctcgata tcagctgtgc ggcttgggcc
      661 ggcgatgaaa acgatgtcgc tgggcatctc gccgtcctcg atgacctcgc caatgcgcgc
      721 gagggcatcg cccaatcccc acacaagctg ggcgaactga gccacgacga tatgtacggg
      781 cgacagcgag ctggccgctc ttgggcccca cggactcgcc gccatcacga tcaacccctg
      841 ctcggccacg agcatgtggg cggtggtaat gcacacatcg cataccgaaa ggtctttctc
      901 gaacggctcc acgcccgcgc cgcgcacgag cgaggtgagc cagggcacgt cgaagcatgc
      961 cacccgccgc agttcgccat cacgcagcag cttcgacaac cggtcagtgg cggtctccga
     1021 gccgggctcc cagaagaagc ggccgcccag tgcctcgaac tcactgcgga agcgttgcac
     1081 catcgcctgc ggcgagggag tctcctccgg cgcg
//
LOCUS       DAOSPL010001591         1113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_132181_length_1113_cov_3.816635, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001591 DAOSPL010000000
VERSION     DAOSPL010001591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132181_length_1113_cov_3.816635"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..1087
                     /locus_tag="PLQ54_21630"
     CDS             8..1087
                     /locus_tag="PLQ54_21630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOS95924.1"
                     /translation="MLDLGGCPLSGIAAKPRVALMELLGIRPDPAHVPDTNSTALDER
                     LLRALDIDTRAVGGILSPEVEGAGQISDSEHVDCWGVRRRFTGKHWEIVESPLADATI
                     DDLDAYPWPDPERIDMAIIERFARAAKRLYEETDTVICGEHPVLGVLELGCWVCGYET
                     FLSRMALDPDFVLRFFDRYFEFQRRVIELYYPAIGPYIHYTSSGDDFATQLASFISPG
                     MFRRLVKPYMTERIRLTRQYTDAAFLHHSCGNVFHLIPDLIDCGVQILNPIQPCAPEM
                     APASLKEAYGDRIVFHGGVDTQGLLPRATPQEVAAATGEMLGVLARHGGYIFAAAHEI
                     QEDVPAENVLATFTAAREYRTRELQ"
BASE COUNT          187 a          373 c          370 g          183 t
ORIGIN      
        1 aagactcatc ctcgacctcg gcgggtgccc actctcgggc atcgcggcca agccccgggt
       61 ggcgctgatg gaactgctgg gcattcggcc cgatcctgcg catgtccccg acaccaacag
      121 caccgccctc gacgagcggc tgctgcgggc gctcgatatc gacacgcgcg cggtgggcgg
      181 gatcctctcg ccggaggtcg agggcgccgg ccagatcagc gactcggaac acgtggactg
      241 ctggggagtg cggcgccgtt tcaccgggaa gcactgggag atcgtggagt cgcctctggc
      301 cgatgcaacc atcgacgacc tcgacgcata cccgtggcct gatccggaac gcatcgacat
      361 ggccatcatc gagcggttcg cgcgggcggc caagaggctc tacgaggaga ccgacaccgt
      421 catctgcggg gagcacccgg tgctcggcgt cctggagctt ggctgttggg tctgcggcta
      481 cgagactttc ctgagccgca tggcgctcga tcccgacttc gtgctgcggt tcttcgaccg
      541 ctacttcgag ttccagcgcc gtgtcatcga gctctattac ccggccatcg gaccctacat
      601 ccactacaca tcgagcggcg atgacttcgc gacacagctc gccagcttca tctcgccggg
      661 aatgttccgg aggctcgtga agccctacat gaccgagcgt atccgattga cgcggcagta
      721 caccgatgcg gcgtttctgc accactcgtg cgggaacgtg ttccacctca ttcctgacct
      781 gatcgactgc ggcgtgcaga tcctgaaccc gatccagcct tgcgccccgg agatggctcc
      841 ggccagcctg aaggaggcct acggcgaccg catcgtcttc cacggcggcg tcgacacaca
      901 ggggctgctc ccacgcgcca cgccgcagga agtggcggcc gccacgggcg agatgctcgg
      961 cgtgctcgcg cggcatggcg gctacatctt cgcggcggcg catgagatcc aggaggacgt
     1021 accggccgag aatgtgctgg cgaccttcac ggcggcgcgg gagtaccgga cacgggagct
     1081 gcagtaggcg gcgatggcgg ccagaggagg ggc
//
LOCUS       DAOSPL010001592         1113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_132192_length_1113_cov_3.247637, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001592 DAOSPL010000000
VERSION     DAOSPL010001592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132192_length_1113_cov_3.247637"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1113
                     /gene="hisS"
                     /locus_tag="PLQ54_21635"
     CDS             <1..>1113
                     /gene="hisS"
                     /locus_tag="PLQ54_21635"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOS95925.1"
                     /translation="LPPESALWQRLESVFRDLCARFRYEEIRTPVFEDTRLFSRGIGD
                     STDIVNKEMYTFTDRGGRSLTLRPEGTASVVRAYVQAKLGGPNHLSKFFYVCSIFRYE
                     RPQQGRYRQSHQLGVEAIGSPGPDIDAEVIGLNAALYAELGVGELAVHLNTIGCQECR
                     PAYRDRLRHQLRSRLDEFCPDCQRRFETNPLRILDCKVPRCGELSADIPSILDALCPA
                     CVAHWDGLRRALDAIDVGYQVDPKLVRGLDYYTRTAFEFKSNAIGSQDSLSGGGRYDG
                     LVELCGGRPTPGIGFAAGIERALMAMERAGAAVAKGTRPSVFVARAGEAARSAALRLM
                     HELREAGIAADTDYFDRGLKAQLRSADSTGAWLALI"
BASE COUNT          158 a          383 c          381 g          191 t
ORIGIN      
        1 tgctcccacc cgagagcgct ctctggcagc ggctcgagag cgtcttccgt gacctctgtg
       61 cgcggttccg ttacgaggag atccggactc ccgtcttcga ggacacccgg ctcttctcgc
      121 gcggcattgg cgactccacg gacatcgtta acaaggagat gtacaccttc accgaccggg
      181 gcggccggag tctgacgctg cgcccggaag ggaccgcctc ggtcgtccgc gcctacgtcc
      241 aggccaagct gggtgggccc aaccacctga gcaagttctt ctacgtttgc tccatcttcc
      301 ggtatgagcg cccccagcag gggcgctacc gtcagtccca ccagctgggt gtggaggcca
      361 tcgggtcccc cgggccggat atcgacgccg aggtcatcgg gctgaatgcc gcgttgtacg
      421 ccgagctcgg agtcggggag ctcgcggtgc acctcaacac catcggttgc caggagtgcc
      481 ggccggccta ccgcgatcgg ctgcgccatc agctgcgctc ccgcctggac gagttctgtc
      541 ccgactgtca gcgccggttc gagaccaacc ccctgcggat tctggactgc aaggtccccc
      601 ggtgtggcga gctgtccgcc gacatcccca gcattctgga cgccctctgc cccgcgtgcg
      661 tggcccactg ggacgggctg cggcgcgcgc tggatgccat cgacgtcggt tatcaggtgg
      721 accccaaact cgtgcggggg ctggactact acacgcgcac ggcgtttgag ttcaagagca
      781 acgccatcgg ttcccaggac tcgctgagcg gcggcgggcg ctatgatggt ctcgtggagc
      841 tctgtggcgg ccggccgacc ccgggcattg gctttgctgc gggcatcgag cgggccctca
      901 tggcgatgga gcgggcgggg gcggccgtgg cgaagggcac tcgccccagc gtcttcgtgg
      961 cccgcgccgg agaggccgcg cgttcggccg cccttcggct gatgcacgag ctccgagagg
     1021 cggggatcgc ggccgacacc gactacttcg accgcggtct gaaggcgcag ttgcgttcgg
     1081 ccgacagcac cggggcatgg ctcgcgctca tcg
//
LOCUS       DAOSPL010001593         1111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_132592_length_1111_cov_2.406250, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001593 DAOSPL010000000
VERSION     DAOSPL010001593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132592_length_1111_cov_2.406250"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="PLQ54_21640"
     CDS             complement(<1..1074)
                     /locus_tag="PLQ54_21640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95926.1"
                     /translation="MTALLGSLVPVLAQMPLAPGDPALTVYVSAQGNDEWSGMVREPR
                     ADGTDGPLASLAGARDAIRERRRRHGGPEPGGYVIEVAGGTYFLDDTFVLGPDDSGTG
                     ASPVTYRAAAGEPVIISGGRPIGDWRETVVDGKALWAAHVPGVAEGEWWFTELFVNGG
                     RRDRTRLPEEGYYHFAGFVSEEEANAPWSTGQTQMRFEGEDLKPWRNLPDVDIIGLTL
                     WISNRMPIESVDTERGIVTFGKPSRFKLSDDHQPRGCRYWVENVFEALNAPGQWYLDR
                     PTGTLYYMPLPGESVDTVQAIAPRLACAVRVEGDPNRGIPVEGVTFEGLTFSHTEWRY
                     PGDSAGDGQAAVSCPGVVVLQSAN"
BASE COUNT          188 a          398 c          346 g          179 t
ORIGIN      
        1 gttcgcgctc tgaagaacga ccacacccgg gcagctcacc gcggcctgac catcgcccgc
       61 actgtcaccc ggatagcgcc actcggtgtg gctgaaggtc agcccctcga aggtaacacc
      121 ctccaccgga atgccccggt tgggatcgcc ttcaacgcga actgcacagg ccaggcgggg
      181 cgcgatggcc tgcacggtgt ccacggactc cccggggagc ggcatgtagt agagcgttcc
      241 ggtcgggcga tcgagatacc actgtcccgg cgcattgagc gcctcgaaca cgttctcgac
      301 ccagtaccgg cagccccgcg gctggtggtc atcgctgagc ttgaaccggc tgggcttccc
      361 gaacgtgaca atgccccgct cggtgtccac tgactcaatg ggcatgcggt tgctgatcca
      421 caatgtgagt ccgatgatgt caacatccgg caggttccgc cacggtttga ggtcctcgcc
      481 ttcgaacctc atctgggtct ggccggtgct ccacggggcg ttcgcttcct cctcggaaac
      541 gaacccggcg aagtgatagt acccctcctc gggcagacgc gttcggtcgc gccgtccgcc
      601 attcacgaac aactcggtga accaccattc gccctcggcg acaccgggta cgtgtgccgc
      661 ccacaacgcc tttccatcga ccacggtctc ccgccagtcg ccgatgggcc ggccgccact
      721 gatgatgaca ggctcacccg ccgccgcccg gtacgtgacc ggactggcgc cagtgcccga
      781 gtcgtccggc cccagcacga aggtatcgtc caggaagtac gtgccgccgg ccacctcaat
      841 cacatagccg ccaggctccg gccccccatg acgtctccgg cgctcgcgga tggcgtcgcg
      901 ggcgcccgcc agtgacgcaa gggggccgtc cgtaccatcc gcacggggtt cgcggaccat
      961 gccggaccat tcatcgttgc cctgagcgga cacgtagacc gtcagggccg gatctcccgg
     1021 cgcgagcggc atctgggcaa gcactgggac gagagagcca agcagcgcgg tcatggcgga
     1081 ggcctcctag cgttcggcga gccggtggac t
//
LOCUS       DAOSPL010001594         1106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_133502_length_1106_cov_2.589914, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001594 DAOSPL010000000
VERSION     DAOSPL010001594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133502_length_1106_cov_2.589914"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PLQ54_21645"
     CDS             complement(<1..203)
                     /locus_tag="PLQ54_21645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95927.1"
                     /translation="MQSDIAQAISDGLASGSPWLVPLAFIAGLCTSLNPCAYPMIGAI
                     AGYMWSRSDRSPRRTAILGIAFVV"
     gene            complement(206..691)
                     /locus_tag="PLQ54_21650"
     CDS             complement(206..691)
                     /locus_tag="PLQ54_21650"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOS95928.1"
                     /translation="MGTYRVVGLVVIAAAVGFVAGTQLSTRRQVAAAGAEGCDTCVPV
                     GGPAADTAAAPAIPANPDRPSLVELGSDECQQCQKMQKVLAELEPVLAGKVDVVRVDT
                     DVHTDVVSRFRLRLIPTQVIVDGKGQELWRHEGFIPLDELKAEIDQAVAASATRQGET
                     K"
     gene            complement(701..>1106)
                     /locus_tag="PLQ54_21655"
     CDS             complement(701..>1106)
                     /locus_tag="PLQ54_21655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95929.1"
                     /translation="ELLQPYVKNIQINVCPSHAGAIGGHNILGSYGYICDGFTLDPND
                     VNYTGLPYRGIGSLAEIRYPSQMIMLGESEKATCRVCPKYHTHGAPSAPPVWPVQERH
                     QGGSNYTFFDGHAKWMKYSSTLAPHNMWKNLD"
BASE COUNT          185 a          365 c          358 g          198 t
ORIGIN      
        1 accacgaagg caatgcccag gattgccgtc cggcggggcg agcggtcgct ccgagaccac
       61 atgtagcccg caatggcgcc gatcatcggg tacgcacatg ggttcagaga cgtgcagagg
      121 ccggcgatga acgccagggg caccaaccac ggactgccgg aggcgagccc gtccgaaatg
      181 gcctgagcga tgtcggactg cacgactact tcgtttcgcc ctgccgagtg gcactggccg
      241 ccacggcctg atcgatctcc gccttcagtt catcgagtgg gatgaacccc tcgtggcgcc
      301 agagctcctg gcccttccca tcgacgatga cctgggtggg gatcagccgg agacggaagc
      361 ggctcaccac gtcggtgtgc acatcggtgt cgacgcggac cacgtccacc ttgcccgcga
      421 gcaccggctc gagctcggcc agcactttct gcatcttctg gcactgctga cactcgtccg
      481 agccgagctc gacgagcgat ggtcgatcgg ggttggccgg gatcgccggc gccgcagcgg
      541 tgtccgccgc cggtccgccc acgggcacgc atgtgtcaca accctctgcc ccggcggcag
      601 ctacctgccg gcgcgtgctg agctgcgtgc cggccacgaa ccccacggcc gcggcgatca
      661 caacgagacc cactacgcga tatgtgccca cgggtgcctc ctagtcgagg ttcttccaca
      721 tgttgtgcgg agcgagcgtg ctcgagtact tcatccactt cgcgtggcca tcgaagaacg
      781 tgtagttgct cccgccctga tgtcgttcct gaacgggcca caccggggga gcactgggcg
      841 cgccgtgcgt atggtacttg gggcacaccc ggcatgtggc cttctccgat tcgccgagca
      901 tgatcatctg cgagggatac cggatttcgg caagcgaccc gatgccgcgg tacggcagac
      961 ccgtgtagtt cacatcgttc gggtcgagtg tgaacccgtc gcagatgtag ccgtagctcc
     1021 cgagtatgtt gtgtccgccg atggcacccg cgtgcgaggg gcacacgttg atctggatgt
     1081 tcttcacgta cggttgcagg agctcg
//
LOCUS       DAOSPL010001595         1104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_133857_length_1104_cov_3.239276, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001595 DAOSPL010000000
VERSION     DAOSPL010001595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133857_length_1104_cov_3.239276"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PLQ54_21660"
     CDS             complement(<1..435)
                     /locus_tag="PLQ54_21660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95930.1"
                     /translation="MRLDVCAWCGTWPFHGVPHSDVDGLLHLMDRAAIDGALVAPLDA
                     VFDRDCRGPNARLARALATRGDRMAFCAMANPRYPGWQETVRIADEDWGAVAVRVIPT
                     YHGYRTDDQSLVELAEVLAERQLPLIVTTRLLDERSHPPAFRV"
     gene            complement(432..>1104)
                     /locus_tag="PLQ54_21665"
     CDS             complement(432..>1104)
                     /locus_tag="PLQ54_21665"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOS95931.1"
                     /translation="TQLIVASLGRSWDYEPPHEHCVRGNRDTLALVERYGGMVLGWAY
                     VNPRFEDEAEAEVRWCVEEAGFVGIKLWVSALASESCVGRMIDLSVELRVPVLMHAWH
                     KATGNLPLESTPQMLAGLAQAKPRARIIMAHVGGDWQLGSRAAEGSPLLVDTSGSIVE
                     NGMIEETVARLGAHRVLFGSDAAGVDLATALAKITAAEIPHEARRRILYDNVVELLSW
                     RRQAA"
BASE COUNT          196 a          394 c          344 g          170 t
ORIGIN      
        1 tacccgaaac gcggggggat ggcttctctc atccaggagc cgagtggtca cgatgagtgg
       61 cagctgccgt tcggcgagga cctcggccaa ctcaaccagg ctctggtcat cggtgcgata
      121 cccgtggtac gtcggaatca cgcggaccgc caccgcgccc caatcctcat ccgcgatccg
      181 gaccgtctcc tgccagccgg ggtacctggg gttggccatg gcgcagaacg ccatccggtc
      241 gccgcgggtc gccagcgccc gagcgagccg cgcattgggg ccgcggcagt cccggtcgaa
      301 cacggcgtcc agcggcgcca ccaacgcccc gtcaatcgcc gcacggtcca tcaggtgaag
      361 gaggccgtcc acatccgagt ggggtacgcc gtggaagggc cacgttccgc accaggcaca
      421 cacgtccagt ctcatgccgc ctggcgcctc cagctgagaa gctcaacaac gttgtcatac
      481 aggattcggc gcctggcctc gtggggaatc tctgccgctg tgatcttggc gagggcggtg
      541 gccaagtcca cgccggcagc gtccgagccg aacagcaccc ggtgcgcgcc cagccgagcc
      601 accgtctcct cgatcatacc gttctccacg atggagccac tggtgtccac cagaaggggg
      661 ctgccttcgg ccgcccggct ccccagttgc cagtcgccac cgacatgggc catgatgatc
      721 cgcgctctcg gcttggcctg cgccaggccg gcaagcatct gcggggtgct ctccagaggc
      781 agattgcccg tggccttgtg ccacgcatgc atcaggacgg ggacgcgcag ctcaaccgac
      841 agatcgatca tacggccgac acaggactcc gaggccaggg cggacaccca gagctttatc
      901 ccgacgaagc cggcctcctc gacacaccag cggacttccg cttccgcctc atcctcaaag
      961 cgaggattga cgtaggccca accaagcacc atgcccccat agcgctccac cagggcgagg
     1021 gtgtcccgat tgcctcgcac gcagtgttcg tgagggggct catagtccca cgagcgtccg
     1081 agcgaggcca cgatgagttg tgtc
//
LOCUS       DAOSPL010001596         1098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_134988_length_1098_cov_2.868648, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001596 DAOSPL010000000
VERSION     DAOSPL010001596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134988_length_1098_cov_2.868648"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1098)
                     /locus_tag="PLQ54_21670"
     CDS             complement(<1..>1098)
                     /locus_tag="PLQ54_21670"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOS95932.1"
                     /translation="ARFAAIGPCPDAAAHAATLASATEAALLEASGPLLKPIPRVDEC
                     PSVWGTTVTAEPIGAPGPGAAHEALCWANADVAVVDPVPPGQATELLVDFGRELVGPL
                     EFEVWASAGTVLDFHLFESIQEGRRQWPETTHNTLRYTCRQGWQQFRGVMRHGFRYAA
                     LHIRGATAPTRIRRLMCRLNTYPVARRGSFECSDDKLTRIYELCAHTTRLCSEDTYVD
                     CPLYEQTFWVGDARNESLVSYTTFGAYDLARRCLELVPESMFRSPIPESQTPSGWQNL
                     LTAWSLFWGWACEEHYVYTGDRDFVARVYPQVRQMLDNLLAMRDARGLLAIEAWNMLD
                     WAPMDTPGAGIVTHNNAELVAALINRGDNIV"
BASE COUNT          177 a          376 c          373 g          172 t
ORIGIN      
        1 tccacgatgt tgtcgccccg attgatgagc gcggcgacaa gctcggcgtt gttgtgagtc
       61 acgatgcccg cgccgggggt atccatcggc gcccagtcca gcatgttcca tgcctcgatg
      121 gccagcaacc cgcgtgcatc ccgcatggcg agcaggttgt ccagcatctg gcgaacctgc
      181 gggtagacgc gcgccacgaa gtcacgatcg cccgtgtaga cgtagtgctc ttcgcatgcc
      241 cagccccaga agagactcca cgccgtcaac aggttctgcc atccgctggg tgtctgtgac
      301 tcgggtatgg gcgatcggaa catggactcc ggcaccagtt cgaggcaccg tcgggccagg
      361 tcgtaggcgc cgaaggtcgt atagctgacc agcgactcgt tccgggcgtc gccgacccag
      421 aaggtctgct cgtacagcgg gcagtcgaca tatgtgtcct cgctgcacag acgcgtcgtg
      481 tgggcgcaga gctcgtagat ccgcgtcagc ttgtcgtcgg agcactcgaa gctgcctcgc
      541 cgggcgaccg ggtacgtatt cagccggcac atcagccggc ggatccgcgt gggcgccgtc
      601 gcgcctcgga tgtgcagagc cgcgtagcgg aacccgtgcc gcatgacgcc acggaactgc
      661 tgccagccct gtcggcaggt gtagcgcagg gtgttgtgcg tcgtctccgg ccactgcctg
      721 cgcccctcct ggatcgactc gaacaggtgg aagtcgagca ccgtccccgc cgaggcccac
      781 acctcgaact cgaggggccc caccagctca cggccgaagt ccaccaggag ctcggtggcc
      841 tggcccggcg gaaccgggtc caccacggcc acatcggcat tggcccagca gagggcctca
      901 tgagcggcgc ccggcccggg ggctccgatc ggttcggccg taaccgtggt cccccagacc
      961 gacgggcact cgtcgacgcg aggaatcggc ttcagcaaag gccccgatgc ctcgagaagg
     1021 gccgcctcgg tggcgctggc cagcgtcgcg gcatgtgcgg cggcgtccgg gcacgggccg
     1081 atggccgcga agcgggcc
//
LOCUS       DAOSPL010001597         1098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_135045_length_1098_cov_2.260786, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001597 DAOSPL010000000
VERSION     DAOSPL010001597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135045_length_1098_cov_2.260786"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLQ54_21675"
     CDS             <1..406
                     /locus_tag="PLQ54_21675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95933.1"
                     /translation="SYVYLYPTATGATSTGWPADRLLFTATERTGLTGYAGVRIYITD
                     PRKGVYEKVTSSSGLWQYRTEYPGGTRFDVTSPEGIPILEGINSTVLSTGPTTWPIGD
                     IAVMTEGLTKPVQVIVESLRHAACGWKGGRER"
     gene            403..>1098
                     /locus_tag="PLQ54_21680"
     CDS             403..>1098
                     /locus_tag="PLQ54_21680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95934.1"
                     /translation="MKRASAFLALAGLAGTLGIGSVGCGTAGGLTDDSFGQVAMGRVV
                     VILQWEQAGTSVRALPSNAQRVELVAVGQTDVGTKAFEVRRTVDRSDFTGSQARVSMD
                     LPIGVGHVISAQAFDVSGNLLVVGSTTVDFTSGSTIQARIVTYLARGLAASDPQAGQV
                     IAYARGAGTSMVIEGVRPDGASLGVLSTSSPPCADPALSPGATRLMYRDAGENLLVSL
                     LGSGAAVSLTGLYP"
BASE COUNT          177 a          374 c          365 g          182 t
ORIGIN      
        1 cagctacgtg tacctctatc ccaccgccac cggcgccacc tcgaccggtt ggccggcaga
       61 ccgactactc ttcaccgcca ccgagcgcac cggcctcact ggctatgccg gggtgcggat
      121 ctacatcacc gacccgcgca aaggcgtata cgagaaggtg accagcagta gtggcctgtg
      181 gcagtaccga accgagtatc ccggcgggac gcgcttcgac gtcacgagtc ccgagggtat
      241 cccgatcctc gaggggatca actccacagt actgtcaacg ggtcccacga cctggccgat
      301 cggcgacatt gccgtcatga ccgaaggtct taccaagccc gtccaggtca tcgtggaatc
      361 gctgcggcac gcagcgtgcg ggtggaaggg ggggcgcgag cgatgaaacg tgcctcggcc
      421 ttcctggcac tggcgggact cgcggggacc ctcgggatcg gctccgttgg atgcggcacg
      481 gccggcggcc tgaccgatga ctcattcggc caggtcgcca tggggcgagt cgtggtgatc
      541 ctccagtggg agcaagcggg gacgtcggtc cgcgctctgc cctcgaacgc ccagagggtg
      601 gaactcgtgg cggtagggca gacggatgtg gggacgaagg cgttcgaggt ccgtcgcacg
      661 gtggaccgga gtgacttcac gggctcgcaa gcccgcgtgt cgatggacct gccgataggg
      721 gtgggccacg tgatctcggc ccaggcgttc gacgtgtccg gcaatctgct ggtcgtcggc
      781 tcgaccaccg tcgacttcac cagtggctcc accatccaag cccgaatcgt cacatatctt
      841 gctcgcggcc tggccgcatc cgacccacaa gccggccagg tcatcgcgta tgcccgcggg
      901 gccgggacca gcatggtcat cgagggcgtc cgccccgatg gcgcgtccct gggcgtcctg
      961 tccacatcga gtccgccgtg tgccgacccc gctctctcgc ccggggccac ccgcctcatg
     1021 taccgcgatg cgggagagaa cctgctcgtc tcgcttcttg ggagcggtgc cgcagtcagt
     1081 ctgacgggcc tgtacccc
//
LOCUS       DAOSPL010001598         1097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_135209_length_1097_cov_2.619002, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001598 DAOSPL010000000
VERSION     DAOSPL010001598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135209_length_1097_cov_2.619002"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PLQ54_21685"
     CDS             <1..524
                     /locus_tag="PLQ54_21685"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOS95935.1"
                     /translation="YLTVRSAGATTAGQQVWAYVCLGPRYPYANWLADFPLVESRVIW
                     WQAYHQKMDGFLYWGLNIWDREHNDRPIDPADGPDLEWSITTGGEYDWLHGDGRLIYA
                     GPDGPIGSIRLANIRDGLEDYEYLWLLAQKAGDVEVARTACLPVTTSLTQFTRDVAVV
                     RAERDAVAAEIEG"
     gene            complement(675..>1097)
                     /locus_tag="PLQ54_21690"
     CDS             complement(675..>1097)
                     /locus_tag="PLQ54_21690"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOS95936.1"
                     /translation="GWPEQSSRIQAVCDWFGPADLATLPDDDDPPNSPQGQLLGNAPE
                     SVPELVRLASPISHVSPDDPPFLIVHGENDFVVPMEQSELLHRALIEAGVESTLVRVA
                     NAGHGLNPTSRDAQPQPPRAEIDQKVLEFFDKHLRPEG"
BASE COUNT          174 a          338 c          410 g          175 t
ORIGIN      
        1 cataccttac ggtcaggtcc gcaggtgcca cgaccgcagg gcagcaggtg tgggcgtatg
       61 tgtgtctggg gccgcgctac ccgtacgcga actggctggc cgacttcccg ctggtggagt
      121 cgcgggtcat ctggtggcag gcctatcacc agaagatgga cggcttcctg tactggggcc
      181 tgaacatctg ggaccgggag cacaacgata gacccatcga tccggccgac gggccggacc
      241 tcgagtggag catcaccact ggcggggagt acgactggct gcacggcgat ggccgcctga
      301 tctacgcggg tcccgatggg cccattggca gcatccggct ggcgaacatc cgcgacgggc
      361 ttgaggacta cgagtacctg tggctcctgg cgcagaaggc gggcgacgtc gaagtggcgc
      421 ggactgcttg tctgccggtg acgacaagcc tgacgcagtt cacgcgggat gtggcggtgg
      481 tgcgcgccga gcgggatgcg gtggcggcgg agatcgaggg ctaggagtcg agagccagac
      541 gccaggggtc agaggggagg cgagtccccc atacgggtag ggggtgatgc ctcgccgcac
      601 ccgacctgaa gctgggcctg taaccgggcc tgccgggtgg cactggacac ccccgggcga
      661 cctgccggtc gcccctaccc ctccggccgc aggtgcttgt cgaagaactc gagcaccttc
      721 tggtcgatct cggcgcgcgg gggctgaggc tgcgcgtctc tcgatgtcgg gttcaggcca
      781 tggccggcgt tcgcgacccg gaccagtgtg gactcgactc cggcttcgat gagcgcacgg
      841 tggagcagct cagactgctc catgggcacg acgaagtcgt tctctccatg cacgatcagg
      901 aacggcgggt cgtcgggcga gacgtgactg atggggctgg cgagccggac cagctcgggc
      961 acgctctcgg gcgcgttgcc gaggagctgc ccctggggcg agttgggcgg gtcgtcatcg
     1021 tcgggcaatg tggccaagtc ggccggcccg aaccaatcgc acaccgcctg gatgcgcgac
     1081 gactgctcgg gccatcc
//
LOCUS       DAOSPL010001599         1096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_135429_length_1096_cov_2.452450, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001599 DAOSPL010000000
VERSION     DAOSPL010001599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135429_length_1096_cov_2.452450"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..>1096)
                     /locus_tag="PLQ54_21695"
     CDS             complement(141..>1096)
                     /locus_tag="PLQ54_21695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95937.1"
                     /translation="PAAGPDEPEPGEPPVMMANASAEAATPAPADVPTVLDGPGNGPS
                     RPLWNGPTTALGLRAVLLERQIDAAVHKDALAQAMKVTPADAAHRIREGQGILSTLLT
                     DELAEEVLAALRRGGVPARAVPADQLVPLPAAIRARKLTWSSSNLVAHPDGGLTPIAV
                     PWDALRVIHCGRVFEPALKARGVSPAPLAFSPIGRVARGAAALASIDSAGIRGGTADG
                     GEIIADLVSLEPAVRLRLDARYLDFSALGPARESTAPPNLRLTLREILSRCPRMTANL
                     GRQELDAQGPFEPPRYEGYDLFDRRTMWMLNLALTREAQDN"
BASE COUNT          145 a          401 c          399 g          151 t
ORIGIN      
        1 cgcgccctct gggcgcaccc ctgtgcggcc ctccgcaaga ccaggcgggc gttccccgcg
       61 accttcgcga gacccagccg cccggcccag ggccctatgc taccgccaat gtccccagcc
      121 ggtgcccgcc gcagtgggcc ctagttgtcc tgggcctcgc gggtgagcgc caggttcagc
      181 atccacatgg tgcgccggtc gaagaggtcg tacccctcgt agcgcggcgg ttcgaagggg
      241 ccttgggcat cgagttcctg acgtcccagg ttggccgtca tgcgagggca ccgggagagg
      301 atctcgcgta gcgtgagccg gagattgggg ggggccgtgc tctctcgcgc aggtcccagt
      361 gcgctgaagt ccagatagcg cgcatcgagc cgcaggcgca ccgccggttc gagggacacg
      421 aggtcggcga tgatctcgcc cccgtcggcc gtgccgcccc ggatgcctgc ggagtcgatg
      481 gaggccaggg cggccgcgcc acgcgccacg cggccgatgg gcgagaaggc cagcggcgcc
      541 ggtgaaacgc cccgcgcctt gagggccggc tcgaacaccc ggccgcagtg gatcactcgc
      601 agcgcgtccc aaggcaccgc gatgggcgtc agtccgccgt ccgggtgcgc caccaggttc
      661 gacgatgacc acgtgagctt acgcgctcgg atggcggcgg gcaggggcac gagctggtcc
      721 gccggcaccg cccgggcggg gaccccgccc cgccggaggg ccgccagcac ctcctcggcc
      781 aactcgtccg tcaggagtgt tgacaggatt ccctgcccct cccggatgcg atgagccgcg
      841 tcggccgggg tcaccttcat cgcctgggcc agcgcgtcct tgtgcacggc cgcatcgatc
      901 tggcgttcca acagcaccgc ccgcagcccg agtgcggtcg tgggaccgtt ccaaagtggg
      961 cgcgagggcc cattccccgg gccatcgagg accgtgggca catcggccgg ggccggggtc
     1021 gctgcctcgg cgctggcgtt cgccatcatg accgggggct cgcccggttc gggctcatcg
     1081 ggcccagccg ccggga
//
LOCUS       DAOSPL010001600         1095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_135561_length_1095_cov_3.812500, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001600 DAOSPL010000000
VERSION     DAOSPL010001600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135561_length_1095_cov_3.812500"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="PLQ54_21700"
     CDS             <1..1014
                     /locus_tag="PLQ54_21700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95938.1"
                     /translation="IQQHGFDQATMYEPAEGLERALALRKALGMTSAMPYSMHGVAGE
                     DVEAFVQGITRKYAGSGVPELLYFNPYLDEPSSPGLLDQAKAFASVVKAAGGKAMLTI
                     DREYARLCGDAVDALNYDVQGSGFRDYVVAVLKGTEQPDPRPESYYWQCLAEDPTVNR
                     FFAGFYLYKSRLDGICPNAFQYLAGNTDPYDDFGGQSVLRPHMVTYPSTNGPVDTVEW
                     EAMREGRDDLRYLVTLQALMAEARAYSTDPRVAEQLRLAEAENELILKTLQADHVLSL
                     SMLAPEWYQQHRWEVAVRTLRLQEALAASAARPSAPPAPIAPPPPLAPPVLGVTPGMA
                     MRG"
BASE COUNT          176 a          378 c          365 g          176 t
ORIGIN      
        1 atccaacagc atggtttcga tcaggccacc atgtatgagc ccgccgaggg actcgagcga
       61 gccctggctc tccggaaggc tctcggcatg accagcgcta tgccgtactc catgcacggc
      121 gtggccggag aggatgtcga ggccttcgtt caggggatca cccgcaagta cgctggctcc
      181 ggagtgcccg agctgctcta cttcaacccc tacttggacg agccaagctc cccggggttg
      241 ctcgaccagg ccaaagcgtt cgcgtccgtg gtcaaggccg ccggcggcaa ggccatgctc
      301 accatcgacc gcgagtacgc ccggctatgc ggggacgctg tggacgccct gaactacgat
      361 gtgcagggca gtgggttccg cgactatgtc gtggccgtgc tcaagggcac cgagcagcca
      421 gacccgcggc cggagtccta ctattggcag tgtctggccg aggatcccac tgtgaaccgg
      481 ttcttcgccg gcttctacct ctacaagtcc cggctcgatg gcatctgccc caatgcgttt
      541 cagtacctgg cgggcaacac ggacccctat gacgactttg gcgggcagtc ggtgctccgg
      601 ccccacatgg tgacctaccc ctcgaccaac gggccggtcg acaccgttga gtgggaggcc
      661 atgcgcgagg ggcgcgatga cctacggtac ctcgtcaccc tgcaggccct catggccgag
      721 gcccgggcct acagcaccga cccgagggtt gccgaacagc tccggctggc cgaggccgag
      781 aacgaactga tcctcaagac tctgcaggct gaccacgtgc tgtcgctgtc gatgctggcg
      841 ccggagtggt accagcagca ccggtgggag gtggcggtgc gaacccttcg gctgcaggag
      901 gccctggcgg cctcggcggc acggcccagt gccccgccgg cgccgatcgc gccaccgccg
      961 ccgttggccc cgcccgtact cggcgtcact ccaggcatgg caatgcgagg ctagccgggc
     1021 cggtcagggg cccgtccgaa ggccgcgcga ggccgcgttc gggtgcgatt cgtcggccat
     1081 gcggccggcc gagtc
//
LOCUS       DAOSPL010001601         1094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_135724_length_1094_cov_4.120308, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001601 DAOSPL010000000
VERSION     DAOSPL010001601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135724_length_1094_cov_4.120308"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..>1094
                     /locus_tag="PLQ54_21705"
     CDS             118..>1094
                     /locus_tag="PLQ54_21705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95939.1"
                     /translation="MNDAHHTYVLPDGSPYFPELQSPWFDAHTDALLENGQLDYAHPD
                     VHAYRVAQIAEILDRYDVDGIDLDFTRSRPWFRPGEEDAGRPLMTTLVERIRRLTDRQ
                     SKSLSMRLEYDPDASIDSGLDIEGWLRQGLFDQVTLGGVGDHTPDAPSDWFVDLAHAW
                     GAKVYPGIEGQLHWLPGPGAGGMGLRPGRGIVDGFGPPSIEYLRAVAALHYMDHADGL
                     SLFNFTCADGPFEVGAFQELVDPEGLALKDKQYVAAVWPWDATIFQAPWLSRFRLDPG
                     RNQAVYPLRLADDFDEAERLGSTPHCLLWLDLMGVNRPDDIEVRLNGARL"
BASE COUNT          177 a          387 c          341 g          189 t
ORIGIN      
        1 cacgtctggc gtatgcaccg cacgctcgcc catctcgact ccttgggtac agaccccctc
       61 cacatcgtgg ccgacgcctg ccacgaagcc ggcatcgcct gccagttcag cctgcggatg
      121 aatgatgccc atcacaccta cgttctgccg gacggctcgc cctactttcc ggagctgcag
      181 tcgccgtggt tcgatgccca caccgacgca cttctcgaga acggacagct cgactacgcc
      241 caccccgatg tacacgctta ccgcgtggcc cagatcgcgg aaatcctcga ccggtacgac
      301 gtggacggca tcgacctgga cttcacgcgg tcgcgcccct ggtttcgccc cggcgaggag
      361 gacgcaggcc ggccgctcat gaccacgctg gtcgagcgca tccgtcggct caccgaccgc
      421 cagagcaagt cgctcagcat gaggctcgag tacgatccgg atgccagcat cgacagcggc
      481 ctcgacatcg agggctggct ccggcagggc ctgttcgacc aggtcacgct ggggggcgtc
      541 ggtgaccaca cacccgacgc tccctcggac tggttcgtcg atctggctca cgcctggggt
      601 gccaaggtct atccgggcat cgaagggcag ctccactggc tcccagggcc cggtgctggc
      661 gggatgggac tccggccggg ccgcggtatt gtcgatggct tcgggccgcc ctcgatcgag
      721 tatctgcggg ccgtggccgc cctgcactac atggaccacg ccgatgggct cagcctgttc
      781 aacttcacct gtgccgatgg cccttttgag gttggcgcat tccaggagct ggtcgacccc
      841 gaaggtctgg ccctcaagga caagcaatac gtggccgcgg tatggccctg ggatgccacc
      901 atcttccagg cgccatggtt gagccggttc cggctcgacc ctggccgcaa ccaggcggtc
      961 tacccgctgc ggctcgccga cgacttcgat gaggccgagc ggctcggtag cacaccccat
     1021 tgcctgctat ggctcgacct gatgggagtg aaccgccccg atgacatcga ggtgcgcctg
     1081 aacggagcgc ggct
//
LOCUS       DAOSPL010001602         1091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_136327_length_1091_cov_3.115830, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001602 DAOSPL010000000
VERSION     DAOSPL010001602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136327_length_1091_cov_3.115830"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..>1091)
                     /locus_tag="PLQ54_21710"
     CDS             complement(122..>1091)
                     /locus_tag="PLQ54_21710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95940.1"
                     /translation="LPWALGLLLSAAEAAMPGLTGEEVRQLRLDAVNLINADRATFGL
                     AAVELDDRATKVGDLFCEAALAQDIRGHFLTDGLSPFLRYAFWGNGTDYTGQNACSTF
                     GMTRAEWDYESVLEVLRRYQVMMMSERPPADWHRRNILNPWHTHVGIGLAWGPTGVRM
                     SQDFVNRYVAVNEIATDQPLDATPLFAGRILEPERFELAGITVFFEDPPRELSVADAN
                     ARMSYSPPAECRHLRVQLPPGQFYTDTRSRGDVVVREDGTFGCRLRFWQGRPGIYGVA
                     VRLRPIGTTGVDDQFYTTYVCLRVYPPRGEDRADGPRSPLALPGQS"
BASE COUNT          184 a          373 c          371 g          163 t
ORIGIN      
        1 gcgctactgc gaggtgctgg gctcccgggg cgggtatatc ctggccccgg cacacctgtt
       61 ccaaccggac atcccgccgg agaacatcct cgcggtctac gcggacgccc cctgacgggg
      121 ttcaggactg cccgggaagg gccaggggac tccgtgggcc gtcggcccgg tcctcgcccc
      181 gtggcgggta caccctgagg cacacatagg tcgtgtagaa ctggtcgtcg acgcccgtgg
      241 tgccgatggg gcgcagccgg accgcgacgc cgtagatgcc ggggcgcccc tgccagaacc
      301 gcagccggca cccaaacgtg ccgtcctcgc gcaccacgac atcgccccgg ctgcgcgtgt
      361 cggtgtagaa ctggccgggc ggaagttgca cgcgaaggtg acggcactcg gccggcggac
      421 tgtagctcat gcgcgcgttg gcatcggcaa ccgagagctc gcggggcgga tcttcgaaga
      481 acaccgtgat cccggccagc tcgaagcgtt cgggctcgag gatccggccg gcgaaaagcg
      541 gtgtcgcgtc cagaggctgg tcggtcgcga tctcgttcac cgccacatag cggttcacga
      601 agtcctgcga catccggacg cccgtcggac cccacgccaa cccgatcccc acgtgtgtgt
      661 gccacgggtt gaggatgttc ctgcgatgcc agtcggcggg cggccgctcc gacatcatca
      721 tcacctggta gcggcgcagg acctccagga cgctctcgta gtcccactcg gcccgcgtca
      781 tgccgaaggt cgagcatgcg ttctgtcccg tgtagtccgt gccattgccc cagaacgcgt
      841 atcgcagaaa cggagagagc ccatcggtga gaaagtggcc ccggatgtcc tgcgcgagag
      901 cggcctcgca gaacagatct cccaccttgg tggcacggtc gtccagctca acagcggcaa
      961 gaccaaacgt ggcccggtcg gcattgatga ggttcacggc atcgaggcga agctgccgga
     1021 cctcttcgcc ggtgagtccc ggcatggcgg cctcggccgc cgagaggagc aggccgagcg
     1081 cccacggcag a
//
LOCUS       DAOSPL010001603         1088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_136884_length_1088_cov_3.535334, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001603 DAOSPL010000000
VERSION     DAOSPL010001603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136884_length_1088_cov_3.535334"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1040)
                     /locus_tag="PLQ54_21715"
                     /pseudo
     CDS             complement(1..1040)
                     /locus_tag="PLQ54_21715"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
BASE COUNT          189 a          405 c          338 g          156 t
ORIGIN      
        1 cgctgctgtc catccagaac catgacctcg atctgagtgc tccccaccgg cgcgcggtac
       61 cgcccgcagt atcggtgcca ctccgccggg gcctccttcg gctccgcggg cttccgccgc
      121 ttgcgcgccc gcgccacggc gggcgccaga atgcccaccg cctcccgcga gacttccccg
      181 gccatcccgc ggccgttggt gaacacggct acaccgatgc cgagctccgg tagcagcagc
      241 aggtccgtgg aaaggcctgg tagcccacca ccgtgtccga tccaccgcgc cccctgggtc
      301 cggccgagag cccaacccag gcctcggccc gattggccgt cgtccgacac ctactgcacc
      361 acctgcatct cgcgtattga agaccgcctt agtacgccct cagcaccggc cggaggacgg
      421 cccaggtgaa gcgagatgaa ccgagccatg tcggcaacac tcgagtacag cccgcccacg
      481 gggctgaagc agcccagctt catctccggg catggcggcc cagcgccgtc gtcgtcggcc
      541 cacgggtaca acgtcgtcac ccgatccgcc aggctgccgg cgggtgtgaa cgagctcgaa
      601 accatgccca gcggtccaag aacctgctcg cctacccaca cctcgtacgg ctctcctgaa
      661 gccctggcca cgatgaggcc cagaagggcg aaggccaggt tcgagtactt ctcttcagtg
      721 aggggcggca caatcagcga catctggggc agactcggga gcagttcctc gatggagggg
      781 aactcgaggg tctcccagca gtcgaaaacc gtctcgcggg ggaaccccgc cgtgtgactc
      841 acggcctggc gaatggtggg cggcgccgcg tcggggaagg ggttctggat ccccagctcc
      901 ggaatccact gtgagatcgg gtcgtccagc ccgagctttc cctcatcgcg aagccgcacc
      961 agggccatgg tcgtgaccac cttggtaatc gacgcgagag ggtagagcgt gtccgcgtcg
     1021 gccgggcgcc cggacgccac atcggcccag ccgaagcccc ccgaccacag agtggcccca
     1081 tcgcacac
//
LOCUS       DAOSPL010001604         1085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_137513_length_1085_cov_2.434951, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001604 DAOSPL010000000
VERSION     DAOSPL010001604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137513_length_1085_cov_2.434951"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PLQ54_21720"
     CDS             complement(<1..316)
                     /locus_tag="PLQ54_21720"
                     /inference="COORDINATES: protein motif:HMM:NF016203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HOS95941.1"
                     /translation="MRFDGFVRPDGRVGTRNHVVVVPTVICAAAVARDVAAAAPGAVA
                     LEQHYGCDFERDLTTRVARVLANTADHPNVYAALFLSLGCEIIQPAMRAEVASLQSAG
                     VRC"
     gene            complement(306..>1085)
                     /locus_tag="PLQ54_21725"
     CDS             complement(306..>1085)
                     /locus_tag="PLQ54_21725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOS95942.1"
                     /translation="AVESGLAALSITDHDTVAGTAEAAAAAEGVGLITIPGVEVSARE
                     GPYDVHILGYFVDTADADLIEFLRLLAEGRLARARVILERLAAMDIHLDFDELTDRAA
                     AHVLGRPHIAALLAEQGHAATPQEAFRQLLRKGAPAYVEPHTVTPEEAVAAILRAGGV
                     AVFAHPGLIGLDHCLRGLVECGLSGVEVYHPYHTPDQVERYAAMAAELGLIATGGSDS
                     HGAVGSASRIGQATCPMEVVEALRLRSRARSSSEEEGPRAV"
BASE COUNT          182 a          414 c          340 g          149 t
ORIGIN      
        1 cgcaccggac tcccgcgctc tggagcgagg cgacctccgc gcgcatcgcg ggctggatga
       61 tctcacaccc gaggctcaag aacagagcgg cgtagacgtt gggatggtcc gccgtgttcg
      121 ccaacacccg ggcaacacgc gtcgtcagat cgcgctcgaa gtcacaaccg tagtgctgct
      181 cgagcgcgac agctcccggc gcggccgccg ccacatcgcg ggccacagcc gcagcacaga
      241 tgaccgtcgg caccaccacc acatggttcc gcgttccaac ccggccgtcc gggcgaacga
      301 agccgtcaaa ccgcacgcgg gccctcctct tcggacgatg acctcgctcg gcttcgcagc
      361 cgcagcgcct cgacgacctc catggggcac gtggcctggc cgatccgaga ggcgctcccg
      421 acggcaccat gggagtcgct gccgcccgtg gcgatgaggc ccagctctgc cgccatcgcc
      481 gcgtaccgct ccacctggtc gggcgtgtga tacggatggt acacctcgac acccgagagc
      541 ccgcactcca ccaggccgcg caggcagtgg tccagcccga tcagacccgg gtgcgcgaag
      601 acggccaccc cccctgcccg aaggatcgcg gcgaccgcct cctcgggtgt caccgtgtgc
      661 ggctcgacat acgccggggc tcccttacgc agcagctggc ggaacgcctc ctgcggcgtc
      721 gccgcgtggc cctgctcggc caacagcgcc gcgatgtgag gccgtccgag aacatgcgcc
      781 gcggcccggt cggtcagttc gtcgaagtcc agatggatgt ccatggcggc cagccgttcc
      841 aggatcactc gagctcgcgc caaccgtccc tcagcaagca gacggaggaa ctcgatcagg
      901 tccgcgtccg ccgtgtcgac aaagtacccc agaatgtgca cgtcgtacgg tccctcgcgc
      961 gccgagactt cgacaccagg tatggtgatg agaccgacac cctccgctgc ggcagccgcc
     1021 tcggcagtgc ccgccacggt gtcatggtcg gtgatgctga gcgccgccaa ccccgactcc
     1081 accgc
//
LOCUS       DAOSPL010001605         1085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_137532_length_1085_cov_2.304854, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001605 DAOSPL010000000
VERSION     DAOSPL010001605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137532_length_1085_cov_2.304854"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PLQ54_21730"
     CDS             <1..812
                     /locus_tag="PLQ54_21730"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOS95943.1"
                     /translation="LILSSEPVLTRQVEARERSGIRAGLDWQLRLCAQADGLQDAATW
                     LGASGTATAGFDCGKDVPKPPSAKGMGGAELSVYFPHTDYGVESGRYASDIRGKVRTS
                     ETWDVVVQTRGSAGAVTLTWPDLRNLPRYYGAVLVDQDSGKQVNMRTTSSYSFEPGET
                     GLRNLRLTVRMADAANALVVLGASKARGTASVTYRTLNDATVDVKVLNAAGRIIRHLV
                     NGDTRVAGTHTESWNGRDDNGTAVPPGFYSIEIRGVFADQTITRATTRVRW"
BASE COUNT          182 a          343 c          387 g          173 t
ORIGIN      
        1 ttttgatcct gtcgagcgag cccgtgctga cccgccaggt ggaagcgcgc gagcggtcgg
       61 gcatccgtgc cggtctcgac tggcaacttc gactctgcgc ccaggccgat gggcttcagg
      121 acgccgcgac ctggttgggg gcgtcgggga cggccaccgc tgggttcgac tgcgggaagg
      181 atgtcccgaa gccgccctca gccaagggga tgggtggggc ggagttgtcc gtctacttcc
      241 cccacaccga ctacggtgtc gagagtgggc gctacgcttc ggacatccga ggcaaggtcc
      301 gcacctccga gacctgggac gtcgtcgtgc agacgcgggg atccgcggga gcggtcacac
      361 tgacgtggcc cgacctccgc aacctgcccc ggtactacgg agcggtcctg gtcgaccagg
      421 atagcggcaa gcaagtgaac atgcgcacca ccagctccta ctcgttcgag ccgggcgaga
      481 cgggtctgag gaacttgcgg ctgaccgtgc gcatggccga cgcggccaat gcactggtgg
      541 tcctgggcgc ctccaaagcc cgggggactg cctcggtgac gtaccgaacg ctgaacgatg
      601 ccacggtcga tgtgaaggtg ctcaatgcgg ccggccgaat aatccggcat ctcgtgaacg
      661 gtgacacgcg ggtggcgggt acgcacaccg aatcctggaa tggccgggac gataacggga
      721 ctgccgtgcc gccgggcttc tacagcattg agatccgcgg ggtgttcgca gaccagacca
      781 tcacccgagc gacgacgcgg gtgcggtggt agatcggttc ggggccccga agccccgaca
      841 cagctgcaag agggagctcg tcaaagcggg acggggggcg gcctggtcca agccaggtac
      901 accccccggg gctagtatac ggccgcgagc cggacgccgg cgcccaaggg gaggaagccg
      961 acatcatgat ctccgttgtt cgtcatccgt ggagcagagg cgtctcctgg ttgttgtgtg
     1021 tgtccctggt ggcgccgctc ctgctcatgg cgtctccggc ccaagcccag tcgctgcggc
     1081 gcgtg
//
LOCUS       DAOSPL010001606         1082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_138081_length_1082_cov_2.766310, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001606 DAOSPL010000000
VERSION     DAOSPL010001606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138081_length_1082_cov_2.766310"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(353..>1082)
                     /locus_tag="PLQ54_21735"
     CDS             complement(353..>1082)
                     /locus_tag="PLQ54_21735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS95944.1"
                     /translation="RYMMAETSYYRPECMMARRLYQDGRFGEIVYCEGEYYHPGIGAA
                     NDGLSTWNGQRTWRWGYPPMLYPTHSTAFLVGVTGERLVRVSCLGETKDDPAFKDNAY
                     DNPFANASALFQTSGGHMFRCNVMWDVWNHGERAQWFGQHMSLYMSAWSGQPHVLKIE
                     GQADVPTIPDFQALLPEPMRIESGHGGSHTYLTHEFVMALLEDREPSVNVYEAVAMTA
                     PGVVAHQSCFKDGEQLTVPSFDPK"
BASE COUNT          186 a          346 c          361 g          189 t
ORIGIN      
        1 cagacgcggc caaccagatg gcggcggtga gtgcaacgag caggctgatc cgtttcatat
       61 actttcccct ccgagcgccc ggcgaggact gccgcctcga gctccagggc acattcctgg
      121 gcgccgcaac ggccccctgt cacccgttga cgagcggagt cccgggaagt tgcggactcg
      181 gcgaccctgg tccggcgtgt cgcggtcgtc tcccccggga tcccgatgag ggccgatgcc
      241 tgatcagggc cccagtgggt atacggacga ggtgccggga agtttcagag gtgccgcgct
      301 ccgctggctg cgggcggcat ggagtctccc gaacacccgc gaaaccaaga gctcacttgg
      361 ggtcgaacga gggtaccgtg agctgctcac cgtctttgaa gcacgactgg tgcgcgacca
      421 cgcctggcgc ggtcatagcc accgcctcat agacgttgac tgagggctcg cgatcctcca
      481 gcaaggccat cacaaactca tgagtcaggt aggtgtgaga tccaccgtgg ccactttcga
      541 tccgcatcgg ctcaggcagc agcgcctgga agtcggggat ggtcggcacg tcggcttggc
      601 cctcgatctt cagcacgtgc ggctggccgg accaggcgga catgtagagg ctcatgtgct
      661 ggccgaacca ctgggcccgc tcgccgtggt tccacacatc ccacatgaca ttgcagcgga
      721 acatgtggcc gccactggtc tggaacaggg ccgacgcgtt ggcgaagggg ttgtcgtagg
      781 cgttatcttt gaacgccggg tcgtctttgg tctcgcccag gcatgacacc cgcacgagcc
      841 gctcaccggt cacgccgacg aggaaggcgg tcgagtgcgt tgggtagagc atgggagggt
      901 acccccagcg ccaggtgcgc tgcccgttcc aggtcgagag cccatcgttg gcggcgccga
      961 tgcccggatg gtagtactcg ccctcgcagt agacgatctc gccaaacctg ccgtcctggt
     1021 agagccgacg ggccatcatg cactcgggtc ggtagtagct ggtctcggcc atcatgtagc
     1081 gc
//
LOCUS       DAOSPL010001607         1082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_138090_length_1082_cov_2.588121, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001607 DAOSPL010000000
VERSION     DAOSPL010001607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138090_length_1082_cov_2.588121"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..79
                     /locus_tag="PLQ54_21740"
     CDS             2..79
                     /locus_tag="PLQ54_21740"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95945.1"
                     /translation="MWGWKIADVVAILGSIDIVLGCVDR"
     gene            103..>1082
                     /gene="nuoH"
                     /locus_tag="PLQ54_21745"
     CDS             103..>1082
                     /gene="nuoH"
                     /locus_tag="PLQ54_21745"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOS95946.1"
                     /translation="MERGPIESLVHRLFAGLYGIPVLGWLIPIVLVLGMVALAALVLI
                     WLERKVSGHIQSRRGPLHNGPHGLLQTVWDALKLLVKEDIVPSSADRLIMLMAPFVVF
                     TPAVLVYVVMPFGKGWTVANLNIGLLWVVSVLSLGPVGIILGGWGSHNRWSLLGGMRS
                     AAQLISYEIPSVLALGAIVMMAGTMSMDGLVAAQDQYEFVLTPPGLIAFVVYLVTALA
                     ELNRNPFDLPEAESELVAGFVTEYSGFKFAIFFLAEFANLLALSMITVTVFLGGWQPP
                     SVLFFLPDSAQLPGVFWFAIKTTVMVFVFQWIKWTLPRLRIDQMLNVCWKG"
BASE COUNT          147 a          306 c          376 g          253 t
ORIGIN      
        1 gatttggggt tggaagatcg ccgatgtggt ggcgatcctg ggcagtatcg acatcgtcct
       61 gggctgcgtc gatcgctaga cccgggggta gagggagcac gcgtggagcg aggcccgatc
      121 gagagtctgg tgcaccgcct gtttgcgggt ctctatggga ttcccgtgct gggctggctc
      181 atccccatcg tgctggtgct tggcatggtg gctctggcgg cactggttct gatctggctc
      241 gagcggaagg tctccgggca tatccagagc cgccgcggcc ccctgcacaa cggcccacac
      301 gggctgctcc agaccgtgtg ggacgcgctc aagctcctgg tgaaggagga catcgttcca
      361 tcgagtgccg accggctgat catgctgatg gcccccttcg tggtgttcac gccggcggtg
      421 ctggtctacg tggtcatgcc cttcggcaag ggctggacgg tggccaacct gaacatcggc
      481 ctgctgtggg tggtttcggt gctctctctg gggccagtgg gcatcattct ggggggctgg
      541 ggcagccata acaggtggtc gctgctgggt ggcatgaggt cggcggccca gctcatctcg
      601 tatgagatcc cgtcggtgct ggctcttggg gccattgtga tgatggccgg gaccatgtcg
      661 atggacggtc tcgtggccgc ccaggatcag tatgagttcg tgctcactcc tccgggtctg
      721 attgcattcg tcgtctacct ggtcacggcc ctggccgagc tgaaccggaa cccgttcgac
      781 ctgcccgagg ccgagtcgga gctggtcgcc ggtttcgtga cggagtacag cggtttcaag
      841 ttcgcgatct tcttcctggc tgagttcgcg aacttgctgg cgctctcgat gatcaccgtg
      901 acggtcttcc tcggcggttg gcagccgccg agcgtgctgt tcttcctgcc ggactcggct
      961 cagcttcccg gtgtgttctg gttcgcgatc aagaccacgg tgatggtgtt cgtgttccag
     1021 tggatcaagt ggactctgcc gcgcttgcgt atcgatcaga tgctcaatgt gtgctggaag
     1081 gg
//
LOCUS       DAOSPL010001608         1081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_138274_length_1081_cov_2.892788, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001608 DAOSPL010000000
VERSION     DAOSPL010001608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138274_length_1081_cov_2.892788"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PLQ54_21750"
     CDS             <1..860
                     /locus_tag="PLQ54_21750"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOS95947.1"
                     /translation="KHYEYDPTMPLDPEVALVRDAGEATVNHVVFSSTNGERVPALVA
                     IPKKGDGPFPALVLLHGYTGSKDDWDKYQVFDLIAGQGVVCIAIDAQLHGERKVEGKD
                     MYDPKDLRSGLDAMIQTIVDCRRAVDYVLTLGQVDPQRIGFVGGSMGAILGAVFCAVE
                     PRVSCCVLVVGGADWGVMVEKSMIGVALRARAAEPIDPDQVREALAPVDPLHFVGHIS
                     PRPVLFQNGTKDIIVPVEANKLLIEACGEPKEVDWYDAGHALPPLKLIPRVFGWLQAH
                     LGFGPGAAE"
     gene            869..>1081
                     /locus_tag="PLQ54_21755"
     CDS             869..>1081
                     /locus_tag="PLQ54_21755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOS95948.1"
                     /translation="MLHHEAEFYERVGANQVQCHLCPHECRIGPGDTGRCGVRRNDAG
                     TLRASAYGAITSLAVDPIEKKPLYHFL"
BASE COUNT          192 a          352 c          353 g          184 t
ORIGIN      
        1 caaagcacta cgagtacgac ccgaccatgc cgcttgatcc cgaggtcgca ttggtgcgcg
       61 acgccggtga agccaccgtc aaccacgtag tcttcagcag caccaatggc gagcgggttc
      121 ccgcgctcgt cgccatcccc aaaaagggcg acgggccctt cccggccctg gtgctcctcc
      181 acggatacac ggggagcaag gacgactggg ataagtatca ggtctttgac ctgatcgccg
      241 gtcagggcgt cgtctgtatc gccatcgatg cccaactcca cggcgagcgc aaggtcgaag
      301 gcaaggacat gtacgacccc aaggacctcc gcagcggcct ggatgccatg atccagacca
      361 tcgtcgattg ccgccgggcc gtcgactacg tcctcaccct cggccaagtg gatccgcagc
      421 ggatcggatt cgtcggcggc agcatgggcg ccatactcgg cgctgtgttc tgtgccgtcg
      481 agccccgggt ctcgtgctgt gtcctggtgg tgggtggggc cgattggggc gtcatggtcg
      541 agaagagcat gatcggcgtg gcgcttaggg ctcgcgccgc cgagcccatc gatcccgacc
      601 aggtgcgtga ggcactggcg ccggtggacc ccctccattt cgtgggccac atcagcccga
      661 ggcccgtgct gtttcagaac gggaccaagg acatcatcgt gccggtcgag gcgaacaaac
      721 tgctcatcga ggcatgtgga gagcccaagg aggtcgactg gtacgatgcc gggcacgccc
      781 tgccgccgct gaagctgatc ccgcgcgtct ttggatggct tcaggctcac ctcggattcg
      841 ggcccggcgc ggccgagtag gtgtacccat gcttcaccac gaggcggagt tctacgagag
      901 ggtcggggcc aaccaagtcc agtgccacct gtgtccgcat gagtgccgga tcggccccgg
      961 ggatacgggc cgttgcggcg tccggcggaa cgatgcgggc acactgaggg catcggcata
     1021 cggtgccatc acctccctgg ccgtggaccc gatcgagaag aagcccctct accacttcct
     1081 a
//
LOCUS       DAOSPL010001609         1080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_138480_length_1080_cov_3.619512, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001609 DAOSPL010000000
VERSION     DAOSPL010001609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138480_length_1080_cov_3.619512"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="PLQ54_21760"
     CDS             complement(<1..1051)
                     /locus_tag="PLQ54_21760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95949.1"
                     /translation="MVGPCNCPIRPDGGVARRATMLRQVRKRGLNPVLVVDAGGLFAG
                     GPYDEYTLGPVVDRERTLTTLRAMALMGYDAVGVGDEELAYGADFLRHAGDVARTTFV
                     SANLCGADGRLLLPTHVVREVGGLRFGIIGATPDEAFLFDEGRFPDGLSVSPPIPAVR
                     AVVEQIRAAVDVVVLLSHLGETTSRELLQQVGGIDVLINAHRMSSEPGGLSDVIGGTV
                     VAHFSYQGRALGRIDFTVDERHRIDGFQVREMRLSDQVESDAEVGALVDAFEARLGGD
                     LAEQSQAVVDLYVTSECPHCRTAQPEVFAAVQDFGPKRVVLRRHYVVHAGEDGALDSG
                     TEPAELLEDRRQACIE"
BASE COUNT          198 a          394 c          337 g          151 t
ORIGIN      
        1 tctcgatgca cgcctggcgt ctgtcctcga gcaactcggc gggctcggtg ccggagtcga
       61 gagcgccgtc ctcgcccgcg tggacgacat agtgacgccg caggacgacc cgcttcggcc
      121 cgaaatcctg aaccgcggcg aacacctcgg gctgagcggt gcggcagtgc gggcactcgg
      181 aggtaacgta gaggtcgacc acggcttggc tctgctcagc gaggtcgccc ccgagccgcg
      241 cctcaaaggc atcgaccaag gcgcccactt cggcatcgct ctcgacctgg tccgaaaggc
      301 gcatctcgcg cacctggaac ccatcgatcc ggtgccgctc atcaacagtg aagtcgatgc
      361 gccccagcgc tcgcccctgg tagctgaagt gcgctaccac cgtcccaccg atcacatcgg
      421 acagcccccc gggctccgag gacatccggt gggcgttgat gagcacgtcg atcccgccca
      481 cctgctgcaa cagctcgcgc gaggtggtct cacccaagtg gctcagcaac accacgacat
      541 ccaccgcggc tcggatctgc tcgacaacgg ccctcacggc cgggatggga ggcgatacgg
      601 agaggccatc gggaaaacgg ccctcatcga acagaaacgc ctcgtccgga gtggcaccga
      661 tgatcccaaa gcgaaggccc ccaacctcgc gcaccacgtg ggtggggaga agcagcctgc
      721 cgtcggcccc gcacaggttg gccgagacga acgtcgtccg ggcgacatcc cccgcatggc
      781 ggaggaagtc ggcaccatag gccaactcct cgtcccccac tccgacggcg tcgtagccca
      841 tcagggccat ggcacgcagt gtcgtaaggg tccgctctcg atcgacgacc ggacccagcg
      901 tgtactcgtc ataggggccg cccgcgaaga ggcctccggc atcgacgacc agcaccggat
      961 tgagcccccg cttccgcacc tgtcgcagca tcgtggcccg ccgcgccacg cctccatcgg
     1021 gccggatggg gcagttgcac gggcccacca tcgaatgcgt ctcgccggtg taggcaatgg
//
LOCUS       DAOSPL010001610         1080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_138490_length_1080_cov_3.375610, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001610 DAOSPL010000000
VERSION     DAOSPL010001610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138490_length_1080_cov_3.375610"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..>1080
                     /locus_tag="PLQ54_21765"
     CDS             111..>1080
                     /locus_tag="PLQ54_21765"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOS95950.1"
                     /translation="MGSEGAGGTTLQQESCGPPIGVLARHCPAVSVRDPLAVALSALR
                     RSTCGAIPVLSEGRFVGLVREADLRQTRADASGTPLLIGDVMQAVVAGIPGHAPPHTA
                     LQIMDSCAADVLPVLDLSGTYRGLLTRSDVLEALSGAVTPDRIGGMATPLGVYLTSGA
                     ARGGPGDLGLFLTGVALFACVAMATAVVFALTWFISPSRAVDLVYAVAGLPGAPVPWA
                     WAAVAAVLGLLFRASPLAAYHAAEHQVVNAMERGLALTPERVSQMPRVHPRCGTNLVV
                     VGVAAATIVTVMGGDVQYVAMAVIIALFLRFTLGGLVQYLITTKPPG"
BASE COUNT          127 a          351 c          398 g          204 t
ORIGIN      
        1 cccgacactt cggaggcctc agtggcccgg gcgtggtaga cttggcaagc cgccccgcgt
       61 ggaacgccga ggtggctgtg ggagttgcga ctggagggag ggatggcgaa gtggggtcgg
      121 agggcgcggg tggcaccaca ctgcagcagg agtcctgcgg cccgcccatc ggggtgctgg
      181 ctcggcattg cccggccgta tcggttcgcg acccactggc cgtggcgctg tcggccttgc
      241 gacgatcaac gtgcggggcg atcccggtcc tgagtgaggg gcgttttgtg gggctcgtcc
      301 gcgaggcgga tctgcgccag actcgggcgg atgcctcggg tacccctctg ctgattggcg
      361 acgtgatgca ggccgtggtg gccggcatcc cagggcacgc gccgccgcat acggcgctcc
      421 agatcatgga ctcctgtgca gccgacgtgc ttcccgtcct ggacctgtcc ggtacctacc
      481 gcggcctgct gacgcggtcg gatgtgctcg aggctctctc cggagcggtt acccccgacc
      541 gcatcggcgg catggcgacc ccgttgggtg tgtacctgac ctcgggcgcg gcccggggcg
      601 ggccggggga cttggggctc ttcctcacgg gcgtggccct gttcgcctgt gtcgccatgg
      661 ccaccgccgt ggtctttgcg ctgacatggt tcatcagccc ttctagggcg gtcgatctgg
      721 tatatgcggt tgccggcctg ccgggcgctc ccgtcccctg ggcttgggct gccgtcgccg
      781 cggtgttggg cctgttgttc cgggcgagcc ctctggccgc ctatcacgcg gcggagcacc
      841 aggtagtgaa tgccatggag cggggactgg cactcactcc cgagcgtgtg tcgcagatgc
      901 cccgagtgca tccccgctgc ggtacgaacc tggtcgtggt cggggtcgcc gcggccacga
      961 tcgtgaccgt catgggtggc gacgtccagt acgtcgccat ggcggtgatc atcgcgttgt
     1021 tcctgcggtt cacgctcggc ggattggtgc agtacctgat cacaaccaag ccacccggcc
//
LOCUS       DAOSPL010001611         1075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_139474_length_1075_cov_3.892157, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001611 DAOSPL010000000
VERSION     DAOSPL010001611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139474_length_1075_cov_3.892157"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1075
                     /locus_tag="PLQ54_21770"
     CDS             <1..>1075
                     /locus_tag="PLQ54_21770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95951.1"
                     /translation="RDPAEVPPALCIATVEAARDIRSMGFWTPDGRPADWHAVMEWVD
                     LLGADTLWVQGPTTDTRTAPLSRGSPWAGANLAALDDLIRECRQRGIRLGVWIPSMRL
                     FGEPSTLPPYRIGGVAARVPAVAVSFLDTQRPKDLADAVKWLCSIQGVDYVGLDYLRD
                     EPQSWDAVDRFIEDLGLDVPKTADGQEMDAQARQAWLRHSWMRWRQDRSVFDMWNWWR
                     AHRMAEIVADIRARAKPDKPLWGFTLGWKHGLEHGQDPFMLMDAGIDLDAVMLYQIEG
                     RDRYEAMMREWSQYTRPEEVGGPEDSASGLELTPPDEAPDEGPEVIPVPIQLVVGDQV
                     DDYHHQRTRETPTPLELYTRIHEA"
BASE COUNT          176 a          354 c          374 g          171 t
ORIGIN      
        1 cgcgatccgg ctgaggtgcc tccggcgctc tgtatcgcca cggtggaggc cgcgcgtgat
       61 attcgcagca tgggcttctg gacccctgat ggccgccccg ccgactggca cgcggtgatg
      121 gaatgggtgg atctgttggg tgccgacacc ctgtgggtcc agggcccgac caccgacaca
      181 cgcacggcgc cgctgagccg cggaagccct tgggccgggg cgaacttggc ggctctggat
      241 gacctgatcc gcgagtgccg ccagcgaggc atccgtctgg gagtgtggat cccgtccatg
      301 cggctctttg gtgagccttc cacgctaccc ccctaccgta tcggcggcgt ggcggccagg
      361 gtaccggcgg tcgcggtgtc cttcctggat acccagcggc ccaaggatct ggccgacgcc
      421 gtcaagtggc tctgctcgat ccagggagtc gactatgtgg gcctggacta cctgcgcgac
      481 gaaccgcaga gctgggatgc cgtcgaccgg ttcatcgaag acctgggcct cgatgtgccc
      541 aagaccgcgg acggccagga gatggacgcc caggcgcgac aggcttggtt gcggcattcg
      601 tggatgcgtt ggcgtcagga tcggtccgtc ttcgacatgt ggaactggtg gcgcgcccac
      661 cgaatggccg agatcgtcgc cgacatccgg gctcgggcca agccggacaa acccctctgg
      721 gggttcaccc tcggctggaa gcacggactg gagcacgggc aggacccttt catgctcatg
      781 gatgccggga tcgacctcga cgcggtgatg ctctaccaga tcgaagggcg cgaccgctac
      841 gaggccatga tgcgcgagtg gagccagtac acgcggccgg aggaggtggg cggccccgaa
      901 gactcagcgt ccggactcga actgacgccg ccggacgagg cccccgacga gggccccgag
      961 gtcatccccg tgcccatcca gcttgtggtg ggtgatcagg tcgacgatta ccaccaccaa
     1021 cgcacccgcg agacccccac gccgctggag ttgtacacgc ggatccacga ggccg
//
LOCUS       DAOSPL010001612         1075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_139483_length_1075_cov_3.450980, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001612 DAOSPL010000000
VERSION     DAOSPL010001612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139483_length_1075_cov_3.450980"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="PLQ54_21775"
     CDS             complement(<1..1018)
                     /locus_tag="PLQ54_21775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95952.1"
                     /translation="MKVVETIPFASELIGPLHTQSYRRAGAVARPGEIELGAPWAIAR
                     QDDGPIVCAAEEHLAGFLEGSMGAPLAASGGRPIRLRVDESLDANPEVHRVSVAEGAI
                     DVVGAGPIGVLQGVFHLEALMRERGGPYVPLGETTRRPLFAHRIHRSALSPFYVEELT
                     GFDGPPFDLRSCGLAVRYPAWTEEDAGPQTYYHDNHLLRMLEHGINGIWLRGALREFA
                     RVSIFPELGARSDAILERLCDLVARAQGFGMKVFLYLNEPMGFPSDSPFWQLYPHLAG
                     VPTRHAAVRTFCTSQPEVLQYLREGTHYVFGQVTGLAGVILISASEYPSHCYCHQSRP
                     DDSAE"
BASE COUNT          183 a          339 c          398 g          155 t
ORIGIN      
        1 gctcggccga gtcatcgggg cgcgactgat ggcagtagca gtggctcggg tactcggagg
       61 cggagatgag gatgacgcct gccagccccg tgacttggcc gaacacatag tgcgttccct
      121 cgcggaggta ctgcagtacc tcgggctgac tcgtgcagaa ggtacggacc gcggcgtggc
      181 gcgtggggac accggcgaga tgtgggtaga gctgccagaa ggggctgtcg ctggggaacc
      241 ccatgggctc gttgagatac aggaagacct tcatcccgaa gccctgggca cgcgccacga
      301 ggtcgcagag ccgttcgagg atggcgtcgg accgggcgcc gagctcgggg aagatgctca
      361 ctcgggcgaa ctcgcgcagc gcaccgcgca gccagatgcc gttgatgccg tgctcgagca
      421 tccgcagcag gtggttgtcg tggtagtacg tctgaggtcc ggcgtcctcc tcggtccaag
      481 ccggatagcg aaccgcgagt ccgcacgagc gcaggtcgaa gggtgggccg tcgaagccgg
      541 tgagctcctc gacgtagaag ggcgacagcg ccgagcggtg gatacggtga gcgaagagcg
      601 gccggcgcgt ggtctcgccg agcggcacgt agggcccccc gcgttcccgc atcagggcct
      661 cgaggtggaa gacgccctgc agcacaccga tgggcccggc acccacgaca tcgatggcgc
      721 cctcggcgac gctcacccgg tggacctcgg gattggcatc gagcgactcg tcgacacgaa
      781 ggcggatggg tctgccgccc gatgccgcaa gcggagcccc catcgacccc tccaggaagc
      841 ccgcgaggtg ctcttcggcc gcgcagacga ttgggccatc gtcctggcga gcgatggccc
      901 acggggcgcc gagctcgatc tcgccggggc gagccacagc gccggcgcgg cggtaggact
      961 gggtatgcag cgggccgatg agctccgatg cgaaggggat cgtttcgaca accttcatgg
     1021 cggactgctc ctctaactcg gtcggttgcc accgcgcttg ggcgaaaggc cgcac
//
LOCUS       DAOSPL010001613         1073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_139907_length_1073_cov_2.875246, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001613 DAOSPL010000000
VERSION     DAOSPL010001613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139907_length_1073_cov_2.875246"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1073
                     /locus_tag="PLQ54_21780"
     CDS             <1..>1073
                     /locus_tag="PLQ54_21780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOS95953.1"
                     /translation="DELLSLVQKPARYIGREWNAIVKDPRGAALRWALCFPDIYDVGI
                     AHLGLRVLYHILNQVEDVVCERVFLPWPDYRALLESTGSPLCSLENQLPLDTFDVVGF
                     SLQYELSYPAVLEMLRLGGIAARARDRGERDPLVIAGGPCALNPEPLADFLDAVCLGD
                     GEDVVLEITECVRQGRSAGAGRGELLRELARIEGLYVPGLYSVEYGADGRVRCIEPQS
                     GVPPVVTRRFVRDLDAAAFPTRPIVPYVEAVHDRVAIEVSRGCTRGCRFCHAGMTYRP
                     VRERTPDTIVRLARESIDATGYDEVSLLSLSTADYSQIEPLCDALLEEFDGQHVSLSL
                     PSLRVDSFSVALCEKVSRVRKSG"
BASE COUNT          154 a          355 c          361 g          203 t
ORIGIN      
        1 gacgagcttc tctcactagt ccagaagccg gctcgctaca tcggccgcga gtggaacgcc
       61 atcgtcaagg atccacgcgg cgctgccctg cgttgggccc tgtgcttccc ggatatctac
      121 gacgtcggca tcgctcacct gggcctgcgg gtgctctacc acattctcaa tcaggtcgag
      181 gacgtcgtgt gtgagcgagt gttcctgcca tggcccgact acagagcgct cctggagagc
      241 actggctccc cgctgtgtag cctcgagaac cagttgccgc tcgacacctt cgatgtcgtg
      301 gggttctcat tgcagtatga gttgagctac cctgccgttc tcgagatgct ccggctggga
      361 ggcatcgccg cccgcgcgcg cgatcgcggc gagcgagacc cgctggtcat cgctggggga
      421 ccgtgcgccc tgaaccccga acccctggct gatttcctcg acgcggtctg tctgggcgat
      481 ggggaggatg tcgtcctcga gatcaccgag tgcgtccggc agggccgctc cgcgggcgcg
      541 ggccgcggcg agctgctccg cgagttggcg cggatcgagg gcctgtatgt gcccgggctg
      601 tactcggtcg agtacggggc cgacggccgg gtccgctgca tagagcccca gtcgggcgtg
      661 ccgcctgtcg tgacccggcg gttcgtgcgc gacctggacg ctgcggcgtt tccgacgcgc
      721 cccatcgtgc cctacgtcga ggccgtgcac gatcgtgtgg ccatcgaagt cagccggggc
      781 tgcacccggg gatgccggtt ctgccatgcc ggaatgacct accggcccgt ccgagagcgg
      841 acacccgata ccatcgtgcg cctggccagg gagtccattg acgcgaccgg gtacgacgag
      901 gtcagcctgt tgtccctctc gacggccgac tatagccaga tcgagccact gtgcgacgcg
      961 cttctggagg agttcgatgg acagcatgtc agcctgtcgc tcccgtcgct gcgcgtcgat
     1021 tccttctccg tggccttgtg cgagaaggtg tcacgagtcc gcaagagcgg cat
//
LOCUS       DAOSPL010001614         1071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_140291_length_1071_cov_4.258858, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001614 DAOSPL010000000
VERSION     DAOSPL010001614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140291_length_1071_cov_4.258858"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1071
                     /locus_tag="PLQ54_21785"
     CDS             <1..>1071
                     /locus_tag="PLQ54_21785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95954.1"
                     /translation="RGAKAVCPEEPEFFSRAGYSGAFRREWQAFYGQPWQDPAGSVAD
                     RYRAERLKVHLETELLRACYEGGRSVDPNVRCFLLAHSPLNYAAWNIVFGHHDALSKL
                     PIDAMVAQVWTGTARTPVPYEGRVAQRTFENAFLEYASSLGLVRGTDVEPWFLMDPLE
                     DNPDRTMEDYRDNYERSLVAALMFPEVTHFETMPWPTRIFGRVPSEFATVIGTAINAL
                     SDMQNQTAAEWDAGPRGIGTFLADSAMWQRGAPSPSDLSCFYGITLPLLMRGIPVEVP
                     HLDRADDPGYLERYRVLFVSYDMLKPMRPEINEALARWAEGGGCLVVLGGDDAYNDVP
                     EWWHERGFASPQDDLLSRCGFDV"
BASE COUNT          171 a          358 c          357 g          185 t
ORIGIN      
        1 tcggggagcc aaagcggtct gcccggagga gcccgagttc ttctcacgag ccggctacag
       61 cggcgccttc cgccgcgagt ggcaggcctt ctatggccag ccttggcagg atcccgcggg
      121 gtcagtcgcc gatcggtacc gggccgagcg actgaaggtg cacctcgaga cggagctgct
      181 gcgcgcctgc tacgagggcg gcaggagcgt cgacccgaac gttcggtgct tcctcctggc
      241 ccattcgccg ctcaactatg ccgcgtggaa catcgtcttc ggccaccacg atgcgctgtc
      301 aaaactgccc atcgatgcca tggtcgccca ggtctggacg ggcacggccc ggaccccggt
      361 cccctatgag ggccgcgtgg cgcagcggac attcgagaac gcgttcctcg agtatgcctc
      421 atcactggga ctcgttcgcg gcaccgatgt cgagccgtgg ttcctcatgg acccgctcga
      481 ggacaacccg gaccggacca tggaggacta tcgcgacaac tacgagcgca gcctggtggc
      541 ggcgctcatg ttccccgagg ttacccactt cgagaccatg ccatggccaa cgcggatctt
      601 cggccgcgta cccagcgagt tcgccacggt gatcgggacc gccatcaacg ccctgtcgga
      661 tatgcagaac cagaccgcgg ccgagtggga cgccggccca cggggcatcg ggaccttcct
      721 ggccgactcg gccatgtggc agcgcggcgc accatcgccc agcgatctga gctgctttta
      781 tgggatcacg ctcccgctgc tgatgcgcgg catcccggtc gaagtgccgc acctggatcg
      841 cgccgacgac ccgggctacc tggagcgcta tcgagtgctg ttcgtgtcct atgacatgct
      901 caagcccatg cggccggaga tcaacgaggc cctggcgcgc tgggccgagg gcggcgggtg
      961 cctcgtggtg cttggtggtg atgacgccta caacgatgtg cccgagtggt ggcacgagcg
     1021 tgggtttgcc agcccgcagg atgacctgct ctcgcgctgt gggttcgatg t
//
LOCUS       DAOSPL010001615         1071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_140331_length_1071_cov_2.700787, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001615 DAOSPL010000000
VERSION     DAOSPL010001615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140331_length_1071_cov_2.700787"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLQ54_21790"
     CDS             <1..384
                     /locus_tag="PLQ54_21790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95955.1"
                     /translation="NAAWDLVDASQEEGFDLASVPEDELPENMRPMSLEERKTYLASQ
                     LATRQQIQRQVVELGRKRMEFIHAELEKQNLDDSKALDFAIRQAVRRQAESKGFTFTE
                     PTRPPEPAAVAPPAPETSPPAAPAK"
     gene            502..>1071
                     /locus_tag="PLQ54_21795"
     CDS             502..>1071
                     /locus_tag="PLQ54_21795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS95956.1"
                     /translation="MATAAARIARGLHAAGVSVQVLTLCDHVPAGLLERDASAGYVVH
                     RLGRLRDPVDALQLAEDTIERLCRAHGATLIHGFYVLHAGYLAVAAAKLLGLRSVVSV
                     RGNDVDRMIFSPPAFGMIEWALRHADAVGCVSTDLVARVSALTGRDDIRYTPNAVDAS
                     RFAPRPADSALRRELFPDAPQEALRWPLLG"
BASE COUNT          188 a          381 c          337 g          165 t
ORIGIN      
        1 aacgcggcat gggacctcgt ggacgcaagc caggaggagg gcttcgacct cgccagcgtg
       61 cccgaggacg agctcccgga gaacatgcgc ccgatgagtc tcgaggaacg caagacctac
      121 ctcgccagcc aactcgccac ccgccagcag attcagaggc aggttgtcga gctgggcagg
      181 aagcgcatgg agtttatcca tgccgaactc gaaaagcaga acctcgatga ctcgaaggca
      241 ctggacttcg ccatccggca ggccgtacgc cggcaggcgg agtcgaaggg cttcacattc
      301 accgagccca cacggccccc ggagccagcg gccgtagccc cgccagcacc tgagacctcg
      361 cctcctgccg cccccgcgaa gtgacccagg gaatcgggcg ccctcctgcc gaaactctac
      421 agaccggaca ggagggcgct cgctctgaac gacctgagtt cgattctggt caccgagaag
      481 taccccccgg acatcggagg cgtggccacg gccgccgcca gaatcgcccg agggctccat
      541 gcggccgggg tgagcgtcca ggtgctcact ctctgcgatc atgtgcccgc aggcttgctg
      601 gaacgcgatg cctccgccgg ttacgtggtg caccggctcg gccgtctgcg tgaccccgtt
      661 gacgcactac aactcgccga agacacgatc gagcggctct gccgcgctca cggcgcgacc
      721 ctcatacacg ggttctacgt tctccacgcg ggctatctcg cggtggccgc agcgaagctc
      781 ctgggcctga ggtcggtcgt cagtgtgcgg ggcaatgacg tagatcgaat gatcttctct
      841 ccaccggcct tcggcatgat cgagtgggcg cttcgccacg ctgacgccgt cgggtgtgta
      901 tcgactgacc tggtggccag agtctcggcc ctgacgggac gcgacgacat ccgctacacg
      961 cccaacgccg tggacgcatc ccgcttcgcc ccgcggcccg cagactccgc cctgaggcgt
     1021 gagctgtttc ccgacgcccc ccaggaggcc ctccggtggc cgcttctggg c
//
LOCUS       DAOSPL010001616         1070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_140540_length_1070_cov_2.580296, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001616 DAOSPL010000000
VERSION     DAOSPL010001616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140540_length_1070_cov_2.580296"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1070
                     /locus_tag="PLQ54_21800"
     CDS             <1..>1070
                     /locus_tag="PLQ54_21800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95957.1"
                     /translation="MSLPAGTYSLLIQTKPPQFIRNEVVHPGQDEVRELPPLGAMLLT
                     LADARGGAVSMSWVAQAVAPDDSTLRDRLRLPPAPAREGAARGLAGLGASTAVTGLTN
                     RPEPIVPGIYHVLVDCQPALDLVCDVEPGRERAVAMPQLAGLCVVLGGLGDESPVRTK
                     FVLCLEEAPGVPVANGLTGGSDEVAMFGPGRYELVAWTAPEIRVPGIELVPGQTRTVE
                     LGSLAGLRVDVGGQFEGRYVVRQINPRTELNEVVGTYAFGRTVLIRPGSYLLEPVGRT
                     PATLSERVFVGGLNDGGALFVDPGLHELQVEVTRLSDGVRVGVFPAATRIELPEGEYS
                     LRPIPADLHLPGTVRLDRRVLT"
BASE COUNT          133 a          390 c          380 g          167 t
ORIGIN      
        1 catgagcctg ccggccggca cctactcgct gctgatccag accaagcccc ctcagttcat
       61 acgcaacgag gtcgtgcacc cgggccagga cgaggtccga gagctaccgc cgctgggtgc
      121 gatgctcctc acactggcgg atgctcgcgg cggcgccgtc agcatgagct gggtggccca
      181 ggcggtggcg ccggatgact cgactctgcg ggaccggctc cggctgccgc cggccccggc
      241 gcgcgagggg gccgcgcgcg gtctggccgg gctcggcgcc tccaccgcgg tcaccgggct
      301 gaccaaccgc cccgagccca tcgtgccggg catctaccac gtcctggtgg actgccaacc
      361 ggccctggat ctggtgtgcg atgtcgagcc gggccgtgag cgcgccgtcg ccatgcccca
      421 gctcgccggc ctgtgcgtgg tgctgggcgg cctcggcgac gaatcgccgg tccgcaccaa
      481 gttcgtgctc tgcctggagg aggcgcccgg tgtgcccgtc gccaacggcc tgacgggggg
      541 ctccgacgaa gtggccatgt tcgggcccgg acggtatgaa ctcgtggcgt ggaccgcgcc
      601 cgagatccgc gtgcccggta tcgagctggt gcccggccag acgcgcaccg tcgagctcgg
      661 ctccctggct ggcctgcgcg tcgatgtcgg cggccagttc gagggacgct acgtggttcg
      721 gcagatcaac ccccgcaccg aactcaacga ggtcgttggc acgtacgcgt tcgggcgaac
      781 cgttctgatt cggccgggct cgtacctcct cgagcccgtg gggcgcacgc cggccacgct
      841 ctccgagcgt gtgttcgtcg ggggactcaa cgatggcgga gccctcttcg tcgacccggg
      901 gctccatgag ctacaggtcg aggtgacccg gctgtccgat ggcgtgcggg tcggcgtctt
      961 ccccgcggcg acgcgcatcg agctccccga gggcgagtac agcctgcgcc ccatcccggc
     1021 cgaccttcat ctgcccggca ccgtgcgcct cgatcgccgt gtgctgacgg
//
LOCUS       DAOSPL010001617         1069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_140686_length_1069_cov_3.543393, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001617 DAOSPL010000000
VERSION     DAOSPL010001617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140686_length_1069_cov_3.543393"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..882)
                     /locus_tag="PLQ54_21805"
     CDS             complement(103..882)
                     /locus_tag="PLQ54_21805"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOS95958.1"
                     /translation="MGMILTLALCAASLAAGDLDPADELPLPPEGKQFRLVWNDEFDG
                     TELDETKWEVPPDAPRRDGWWMRQAITLDGRGHLVISTLKDGDRYVDGCVRTRGKFEH
                     AFGYYVARIRLQSEPGHWPAFWLYNHCEGNIGNGGVDGAEIDIMEKPWLDGRVNHAIH
                     WDGYGAEHQSEGKVADVAGLMDGYHTYALWWSPTDYVFYIDGQETWRTRAGGICREPL
                     YIKLSDEIGTWGGDIGAANLPDSFLVDYVRVYDMVDAGPAE"
BASE COUNT          176 a          377 c          337 g          179 t
ORIGIN      
        1 agcgagcgag ctggaccgtc aacacggccg cggcccggca cccgagccat ctcggtcgtc
       61 cgcgaggccc tgagtctcct cggccaccga gggtcgcggc cctcactcgg cgggacccgc
      121 gtcgaccatg tcgtagacgc gcacgtagtc cacaaggaag gagtcgggta ggttggccgc
      181 cccgatgtcg ccgccccatg tgccgatctc gtcgctcagc ttgatgtaca gtggctccct
      241 gcagattccc ccagctcgcg tccgccacgt ctcctgaccg tcgatgtaga acacatagtc
      301 tgtgggcgac caccacaggg cataggtgtg gtacccatcc atgaggccgg ccacatcggc
      361 caccttgccc tccgactggt gctccgcccc gtagccatcc cagtggatcg cgtggttgac
      421 gcgaccatcg agccagggct tctccatgat gtcgatctcg gcgccgtcga cgcccccgtt
      481 gccgatgttg ccctcacagt ggttgtacag ccagaaggcg ggccagtggc cgggttcgct
      541 ctgcagccgg atgcgcgcca catagtagcc gaacgcgtgc tcgaacttgc cgcgcgtccg
      601 cacacagccg tcgacgtaac ggtcgccatc cttgagcgtg ctgatgacga ggtggccccg
      661 gccatccagc gtgatggcct ggcgcatcca ccatccgtcg cgccggggtg catcaggtgg
      721 gacctcccac ttggtctcgt cgagctcggt gccatcgaac tcatcgttcc acacgaggcg
      781 gaactgctta ccctcgggcg ggagcggaag ctcgtcggca gggtccagat cgcccgcggc
      841 cagcgaggcc gcacacagtg ccagtgtgag gatcatcccc atcgtcgaac acccccggta
      901 gacacaggcc ggagcgcact caggcggcgc tccggcccat acacccacca tggccgtggc
      961 tagacgaaag gcgtcacgcc gggcttggcc acctcgggtt ccggcagcgt ggcatccatg
     1021 tcgtagacgg gcggcgagag atcgagctgt gagttcatga cctggtccc
//
LOCUS       DAOSPL010001618         1068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_140897_length_1068_cov_3.550839, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001618 DAOSPL010000000
VERSION     DAOSPL010001618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140897_length_1068_cov_3.550839"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1068)
                     /locus_tag="PLQ54_21810"
     CDS             complement(<1..>1068)
                     /locus_tag="PLQ54_21810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOS95959.1"
                     /translation="DSTGPIRVALVIGQLVSGGAETQLIALAKGLDQSPDFSVCVYCL
                     SRDAPRAPELTDSGIAVHYVTRGLLDGFSRIVPLIRLYREHRIDVAHCWLPVANLWGK
                     LSASLAHVPVIIHGERIATPPRQRLNRVGQRFLLRQTTCFVGNSTAVRDMLIRHKLAI
                     PERIRVIPNGIRPETWRSGKPRDEVLRALGLPTDRPVVGMVSRLVAAKNVPCLVRAAA
                     RLQKRQPPPVFWVAGDGPARPEAEAEVARRGLQDSFFFLGDRRDVPQLMNAMDVSCLC
                     SHYEGFPNAVIEAMAAGKPVVATNTPGTDQVVTDEETGLLSPDDDDEALARNIDRVLS
                     DEALRTRLIQAGTALIENRYT"
BASE COUNT          183 a          328 c          376 g          181 t
ORIGIN      
        1 atggtatagc ggttctcgat cagagctgtg ccggcctgaa tgaggcgggt ccggagtgcc
       61 tcatcggata ggacccggtc gatgttgcgg gccagggctt catcgtcatc atctggactc
      121 agaagccccg tctcttcgtc ggtgaccacc tggtcggtgc cgggggtgtt cgtggccacc
      181 accggtttcc ccgcggccat ggcttcgatg actgcgttgg ggaagccctc gtagtgggag
      241 cacaggcacg agacgtccat ggcgttcatg agctggggca catccctgcg atctcccagg
      301 aagaagaagg agtcttgcag gccccgccga gccacctcgg cctcggcctc gggacgcgcg
      361 ggcccatccc cggccaccca gaacaccggc ggaggttggc gtttctgaag ccgggccgcg
      421 gcccggacga ggcacgggac gttcttggcg gccaccaggc ggctgaccat ccccaccacc
      481 ggacgatcgg tgggcaatcc cagggcgcgc agtacttcat cgcggggctt gccggagcgc
      541 catgtttcgg gccggatgcc gttggggatc acgcggatac gctccgggat ggcgagcttg
      601 tgacggatca gcatgtcccg aactgccgtc gagtttccca cgaagcacgt ggtctgccgc
      661 aggaggaacc gttgacccac ccggttgaga cgctgacgcg gaggtgtggc gatacgctct
      721 ccgtggatga tcaccggcac gtgggccagg ctcgcggaca gcttacccca gagattggcc
      781 acggggagcc agcagtgggc cacatcgatt cggtgttcgc gatagagccg tatcagtggg
      841 acgatgcgtg agaagccgtc cagcagcccg cgggtcacgt agtggaccgc gatcccggaa
      901 tcggtgagct cgggcgcccg gggggcgtct cgggacaggc agtagacgca tacggagaaa
      961 tcaggggatt ggtcgagccc tttggccagt gcgatgagct gggtctcggc tcctcccgag
     1021 accaactgac cgataaccag agccacccgg atgggcccgg tggagtct
//
LOCUS       DAOSPL010001619         1064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_141779_length_1064_cov_3.075322, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001619 DAOSPL010000000
VERSION     DAOSPL010001619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141779_length_1064_cov_3.075322"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..>624
                     /locus_tag="PLQ54_21815"
     CDS             35..>624
                     /locus_tag="PLQ54_21815"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95960.1"
                     /translation="MGVRRREIIEYLDSLGQPYRSDRTNESLMAARNLVRNRLLPVWL
                     GAGLGDPVEALRRLAERMCLDDEALMWAADQFLTESRTAGECVEGQECRIALQRLTGL
                     PDAVGMLVLRRACERVSGGLHGLSLAHVREIWKMVGSARVHARVVLPIGVRASIAPGE
                     VRIQRLPKGGSSTSEVCESVPRVRLNVPGRTRVHELG"
     assembly_gap    625..634
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <635..998
                     /gene="tilS"
                     /locus_tag="PLQ54_21820"
     CDS             <635..998
                     /gene="tilS"
                     /locus_tag="PLQ54_21820"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOS95961.1"
                     /translation="GVVFEAALGRACEPVCGVRHGHATWLRAAGTCDSWSVRPWQPGD
                     RVRPVGLDGHSKKLKKLFQERRIPPAERSRYPVVVDAADTPVWVPGLAADQRWACADA
                     APGAVHLHMVWVAGRPGV"
BASE COUNT          159 a          297 c          412 g          186 t
ORIGIN      
        1 tccggccagc cccgatggtc gggtgcgacc attcttgggg gttcggcgta gggagatcat
       61 cgagtacctg gattcgctcg gacagccgta ccgcagcgat cgcacgaatg agagcctgat
      121 ggccgcaagg aacctggtgc gcaacagact gctcccggtc tggctgggag cggggcttgg
      181 ggaccccgtc gaggccctga ggcgcctggc cgagaggatg tgcctggacg acgaggccct
      241 gatgtgggcg gcggatcagt tcctgaccga atcgaggacc gcgggcgagt gtgtcgaagg
      301 acaggagtgt cgcatcgcgc tacagaggct gacgggcttg cccgatgccg tgggcatgct
      361 ggtactgcgg cgcgcgtgtg agcgcgtgtc cggcggcctt cacgggctgt cactggctca
      421 cgtgcgggag atctggaaga tggtcggttc tgcccgggtt catgcccgtg tggtcctgcc
      481 gattggggtc cgtgcgagca tcgctccggg cgaagtccgg atccagcggc tgccgaaagg
      541 tggctcatcg acgtcggaag tatgcgaatc ggtgccgcgc gtccgactga acgtgccagg
      601 tcgaacccgc gtccatgagc ttggnnnnnn nnnntggagt ggtgttcgag gccgctcttg
      661 ggcgagcttg cgaaccggtc tgtggcgtgc gtcacgggca cgccacgtgg ctgcgggcgg
      721 cgggtacgtg cgactcgtgg agcgtgcggc cgtggcagcc gggtgaccgt gtgcggcccg
      781 tggggctgga tggacactcg aagaagctga agaagctgtt ccaggagaga cggatcccac
      841 cggccgaacg aagccgctat ccggtcgttg tggatgcggc ggacacgccg gtgtgggtac
      901 cggggctggc ggcggaccaa cgctgggctt gtgcggatgc ggcgcccggt gcggtgcatc
      961 tgcacatggt ctgggtcgcc ggacggccgg gggtctagga ccagcgggaa cctgaggggt
     1021 ggcaaccttt gccatcgagg tacatcctac cgatcgtgct catc
//
LOCUS       DAOSPL010001620         1062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_142174_length_1062_cov_4.186693, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001620 DAOSPL010000000
VERSION     DAOSPL010001620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142174_length_1062_cov_4.186693"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            397..>1062
                     /locus_tag="PLQ54_21825"
     CDS             397..>1062
                     /locus_tag="PLQ54_21825"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOS95962.1"
                     /translation="MHGHRGCVCRTTLWMGWTVIAVALSGAWAQAAVFTPADVQARTL
                     DNGVRVVVREAHEAPIVALDVYVRFGSALETDETNGVAHTIEHLVFQGMSGSRVGRLP
                     RLIESLGGRVTAETSRDHTHFAVALSPDGLEEAATQLAVAIRNPETDPTIVARERNII
                     AHELAQQADNPVLQLQELSYRLGFTKHGYRLSPGGTRDSLANLSRADIQRAHDAYYTG
                     PNTA"
BASE COUNT          176 a          376 c          356 g          154 t
ORIGIN      
        1 gggcacgcac agcctcccgt tctcacacgt gatccggtgg ccatcggcgg ggggcaccag
       61 tcgttcgaac gagggggact gatcagacat ccggggttcc tctcttgtct cgtcgcccgg
      121 gttgcccggc gagcggggcg gtgagggcgc tgcaggccaa tcccacgtgg ccccgcactg
      181 tcggtgtcgg cgggcgccac tggcccgaag aagatcatac cttccgcgtc cccccgggtc
      241 cacgcccgcc cccggcgagg ccgcactgca gcatgcggaa tgcccggatc gagtcccggg
      301 ctgtcctgga ggaaagcgcc ggaggcctgc gaacaaccgt ccagccccag ggcgtgcgcg
      361 agagcgtggc gccgggcacg ctaaggagcc cccaccgtgc acggacaccg cggatgtgtt
      421 tgccgcacca cattatggat gggctggacg gtcattgccg tggcgctctc aggcgcgtgg
      481 gcacaggcag ccgtgttcac gccggcggac gttcaggccc ggacgctcga caacggcgtt
      541 cgtgtggtcg tccgggaggc ccacgaagcg cccatcgtgg ccctcgatgt ctacgtccgc
      601 ttcgggtcgg ccctggagac ggacgagacg aacggcgtgg cccacacgat cgaacacctc
      661 gtcttccagg ggatgtccgg atctcgggtg ggacgattac ctcgcctgat tgagagcctg
      721 ggcggccgcg tcactgccga gaccagtcgc gatcatacgc acttcgcggt cgccctatcg
      781 cccgatggcc tggaggaggc cgccacacag ctggccgtcg ccatccggaa ccccgagacc
      841 gacccaacca tcgtggcgcg ggaacggaac atcatcgccc acgagctcgc gcagcaggcg
      901 gacaacccgg tattacagct tcaggagctc tcataccgcc tcggcttcac gaagcacggg
      961 taccggctca gcccgggtgg cacgcgcgac tcgctggcga acctgtctcg ggccgacatc
     1021 cagcgcgcgc acgacgcgta ctacacgggc ccaaacacgg cc
//
LOCUS       DAOSPL010001621         1057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_143385_length_1057_cov_1.845309, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001621 DAOSPL010000000
VERSION     DAOSPL010001621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143385_length_1057_cov_1.845309"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1057
                     /locus_tag="PLQ54_21830"
     CDS             <1..>1057
                     /locus_tag="PLQ54_21830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95963.1"
                     /translation="MSYEGHVTRLTADGELPWGDADNPKVYSSTYETSAPKIASDGQG
                     GAVLVWMDYNESDEYVLCVQRVDGDGNFLWNEGSPVYLSETGDADCPRIASTGDGGAI
                     VMWYEDPDGYGVYGQKINADGGAEWDEGGVLIDTMANLSTDSLDVAYDGAGGGYFTWV
                     DDDTVYAQHIDDSGELAWESPVMMSLESEVEDVAQHPRKTVEDGLGGFITAWYNGDNQ
                     VKAQRCGADGNVLWGEGGAVLSNGVSIYYGPKLASNGQGGAVAVWIDNRDVELDALSE
                     VALEADGDTGQDVFIQGINADGELGDPGYTAAPETYRHHGNNEVLCFVSTAHTSSNYA
                     GILILTAVCFGLCCLLRR"
BASE COUNT          212 a          300 c          347 g          198 t
ORIGIN      
        1 catgagctat gaagggcatg tcacccggct gaccgctgac ggcgagctgc cctggggaga
       61 tgcggacaac ccgaaggtgt actcgtccac atatgaaacc tcggcgccga agatcgcctc
      121 agacggtcaa ggcggcgcgg ttctcgtgtg gatggattat aacgaatccg acgagtatgt
      181 tctgtgcgta cagcgggtgg atggcgacgg aaacttcctg tggaacgaag gctcccccgt
      241 gtacctcagt gagacgggcg atgccgactg tccccggatc gcctccacgg gagacggagg
      301 tgccatcgtg atgtggtatg aggatcccga cggctacggg gtctacggcc agaagatcaa
      361 cgcggacggc ggggccgagt gggacgaagg cggcgtgctc atcgatacca tggcaaacct
      421 ctctactgat tctctcgatg tggcctatga cggagcaggc ggcgggtatt tcacctgggt
      481 cgatgacgac actgtttatg ctcagcacat cgacgattcg ggcgagctcg cctgggagtc
      541 gccggtgatg atgagcctcg agtcagaagt ggaagacgtt gcccagcatc cgcgcaagac
      601 ggtcgaagac ggactcggcg gcttcatcac ggcctggtac aatggtgaca atcaggtcaa
      661 ggcccagcgc tgcggcgccg acgggaacgt gctctggggt gaaggcgggg cagttctctc
      721 caacggcgtg agcatctact acgggcccaa gctcgcttcc aacggccagg gcggtgcagt
      781 ggcggtctgg atcgacaacc gcgatgtgga gctcgatgcc ctttcggagg tggcgctcga
      841 ggcggacggt gatacgggcc aggatgtctt catccagggc atcaacgccg acggcgaact
      901 gggtgatccg ggctataccg cggccccgga aacctacaga caccacggca acaacgaggt
      961 attgtgtttc gtatccactg cccacacttc ttcgaactac gcaggcatac tcatcctgac
     1021 cgcagtatgc ttcggtttgt gctgtctcct ccgtcgc
//
LOCUS       DAOSPL010001622         1053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_144133_length_1053_cov_2.682365, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001622 DAOSPL010000000
VERSION     DAOSPL010001622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144133_length_1053_cov_2.682365"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1053
                     /locus_tag="PLQ54_21835"
     CDS             <1..>1053
                     /locus_tag="PLQ54_21835"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOS95964.1"
                     /translation="RQGYATAYIGKWHLDGPRRGGFTPPGPRRHGFDYWAAANCTHDY
                     MHSHYYRDDPQPIWIEGYDADHYTGLAVRYIERHAARGPFCLFLSWGPPHDPYALMPP
                     EYRVYRPEDIVLPPNCTLDTRDDLAGYYSHITALDRCFGRILGALAATGVADDTIVVF
                     TSDHGDMLGSQGCHRKQRPWDESIRVPFALRYPGRVPSGLRTPVLLNVVDLMPTMLSL
                     AGVAPPSSCEGLDLSHVACGSERGARPTSAFLQHCCTFADARDLPVWRGVRTERYTYA
                     ETRDGPWLLYDNDQDPHQLHNRVADADPVWRRQARTLRDELHAWMDRVDDDFAPREDY
                     WRRFGYTVDPYAQAPYT"
BASE COUNT          173 a          388 c          311 g          181 t
ORIGIN      
        1 cgccagggct atgcgacggc ctatatcggc aagtggcacc tcgacggccc ccgccggggc
       61 gggttcacgc cccccggccc gcgccgccat gggttcgatt actgggccgc cgccaactgc
      121 acgcatgact acatgcactc acactactac cgcgacgacc cacagcccat ctggatcgag
      181 ggttatgatg cggaccacta caccggcctg gccgtccgct acatcgagcg ccacgccgct
      241 cgcggaccgt tctgcctgtt tctgtcatgg gggccgccgc acgatccata cgcactgatg
      301 ccccccgagt accgcgtcta ccgccccgag gacatcgtcc ttcctcccaa ctgcaccctg
      361 gacacccggg atgatctagc tggctactac tctcacatca cagccctcga ccgctgcttc
      421 ggccgcatcc tcggcgctct ggcagcgacc ggggtcgccg atgataccat cgtcgtgttc
      481 acttccgatc acggggacat gctgggctca cagggctgtc atcgcaagca gaggccgtgg
      541 gatgagtcca tccgggtgcc cttcgccctg cgttacccgg ggcgggtgcc atccggcctt
      601 cgaacgcccg tgctgctgaa tgtggtggac ctcatgccca ccatgctctc gctggccgga
      661 gtggcgccgc ccagttcttg cgagggcctc gatctcagcc atgtggcgtg cggatcagaa
      721 cgaggcgcac ggcccacttc agccttcctg caacactgct gcaccttcgc cgatgcccgc
      781 gaccttcccg tctggcgagg ggtgcgcacc gagaggtaca cgtatgcgga gacacgagac
      841 gggccctggt tgctctatga caacgaccag gacccgcacc agttgcacaa ccgtgtggcc
      901 gacgccgatc ccgtatggcg gcggcaagct cgcacgttgc gcgacgagct acacgcatgg
      961 atggaccggg tcgacgatga cttcgccccc cgcgaggact actggcggcg cttcggctac
     1021 accgtggacc cgtatgccca ggcgccctac acc
//
LOCUS       DAOSPL010001623         1051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_144580_length_1051_cov_2.780120, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001623 DAOSPL010000000
VERSION     DAOSPL010001623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144580_length_1051_cov_2.780120"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLQ54_21840"
     CDS             complement(<1..199)
                     /locus_tag="PLQ54_21840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95965.1"
                     /translation="MAVPEFAVWGWFIALVLAVVAVRARRSLSSEPKPGLSSFLELCF
                     DGLSNLARSFIGPGYEKHLPFV"
     gene            complement(388..747)
                     /locus_tag="PLQ54_21845"
     CDS             complement(388..747)
                     /locus_tag="PLQ54_21845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95966.1"
                     /translation="MVALCSWGLGWRVWSAALLGIGLGLGLLRLTEWAVLAATQPSRL
                     RRNLLTGVFLAKLPVMGLVFYVITQHTELNAGALAGGLGVPLAVALLKLAGQALSPPA
                     PTPNGNATSKETRGHGL"
BASE COUNT          176 a          378 c          348 g          149 t
ORIGIN      
        1 ccacgaaggg cagatgcttc tcatagcccg gaccgatgaa gctccgggcg aggttgctca
       61 ggccgtcgaa gcagagctcc aggaagctcg agaggcccgg tttgggctcg ctggagaggc
      121 tcctgcgcgc ccgaaccgcc accacggcga gcaccagggc gatgaaccat ccccatacgg
      181 cgaactcggg aacggccacg ccggtgctgt gttcgacggc ccggagagga gcgtagagta
      241 tgccgccatg ggcctcatgc tccgatgcgg cctcatgcgc gccaccggcg ggcgcggagt
      301 gggcagtatc ggcttcagcg gcgtgtggcc cgctccccgg ggatgccccc accgccgtgg
      361 caaggctcgc gaccggtatt gacccactca cagcccgtgg cctctcgttt cctttgaggt
      421 ggcgttgccg ttcggcgtcg gggcgggcgg gctgagcgcc tgtccagcca gcttgagcag
      481 cgccaccgcc agcgggactc ccaggccgcc cgccagtgcc ccggcgttca gctccgtgtg
      541 ctgggtgatc acgtagaaca ccagtcccat caccggtagc ttggcgagga acacgccggt
      601 cagcaggtta cgcctcagac gactgggctg ggtcgcggcg agcacggccc actctgtgag
      661 gcgcagcagg ccgaggccga gcccgatccc cagcagcgcc gccgaccaga ccctccaccc
      721 cagaccccag gagcagagag cgaccacgac cagcggccca aaccatgcgg tggtccgatg
      781 gacgcgcctc cagaaccccg catcgatgca cagcgccgcg atggctggac acgcccctca
      841 cgactgttgt tggctcagtg accataccgc aaaccgcggg cgtcgtctat cggactccgc
      901 cgttgttacg cgtccattcg caatgattca ctcagtcgtg acactagcgc tccggaggcc
      961 gcgggcggtt ggggcgcggc cggtctgcca gacgcatcag ctcgcggaag gccgccgcgg
     1021 ctcccagtcc gaacccgacc agcgtgcccc a
//
LOCUS       DAOSPL010001624         1051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_144585_length_1051_cov_2.670683, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001624 DAOSPL010000000
VERSION     DAOSPL010001624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144585_length_1051_cov_2.670683"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="PLQ54_21850"
     CDS             <1..998
                     /locus_tag="PLQ54_21850"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS95967.1"
                     /translation="PAAIRAIDLGKHVFAQKPLAHNIRECAELARAARERGVLTQMGN
                     QGHCGEGFRRACEDLWAGVVGDVTETHSLLGRNFGGTGGRPETKPIPAGLHWDEWIGP
                     APYRDYHDGLHPFSWRNWRDFGTGTIGDMACHNVDCVFFGLKVNEAKTYTIECLNTNG
                     GSEEMCAQNNIVRYDIPERAGMPALKVYVYDHGDLKSDVMKEAEKEFGIELTEDTLYI
                     GSKGFYRTGGHAGDAVILSPDRAKEWEKPAEVLPRVNGSPIHDLYDAIRNGTTPASNF
                     PDAATPLTSFVLSGQLAQQAGVGKKVEWDVEKMECTNMPELNQYVRRTYRPGWEV"
BASE COUNT          203 a          355 c          340 g          153 t
ORIGIN      
        1 cgccggccgc gatccgcgcc atcgacctcg ggaagcacgt cttcgcgcag aagcccctgg
       61 cccacaacat tcgcgagtgc gccgagttgg ctcgcgcggc ccgggagagg ggcgtcctca
      121 cgcagatggg caaccagggc cactgcggcg aggggtttcg ccgtgcgtgc gaggatctgt
      181 gggccggagt cgtgggggac gtcaccgaga cccatagtct cctgggccgg aacttcggcg
      241 gcaccggcgg gcggccggag accaagccca tccccgcggg cctgcactgg gatgagtgga
      301 tcggccccgc gccctatcgc gactaccacg acgggctgca cccgttcagc tggcgtaact
      361 ggcgcgactt cggcacgggc accatcggcg acatggcctg ccacaacgtc gactgcgtct
      421 tcttcggcct gaaggtcaac gaggcaaaga cctacaccat cgagtgcctg aacaccaatg
      481 gcggcagcga ggagatgtgc gcgcagaaca acatcgtccg atacgacatc cccgagcgcg
      541 ccggaatgcc cgcgctgaag gtctatgtgt atgaccatgg cgacctcaag tccgatgtca
      601 tgaaggaagc cgagaaggag ttcgggatcg agctcacaga ggacaccctc tacatcggca
      661 gcaagggctt ctaccgaacc ggtggccatg ccggcgatgc cgtcatcctc tcgcctgacc
      721 gcgccaaaga gtgggagaag cctgccgagg tgctcccccg agtcaacggg agcccgatcc
      781 acgacctcta cgacgccatc cgcaatggca ccacgcccgc gtcgaacttc cccgacgccg
      841 ccacgccact gacttcgttc gtactgtcgg gccaactggc gcagcaggcc ggcgtcggca
      901 agaaggtcga gtgggacgtc gagaagatgg aatgcaccaa catgccggag ctgaaccagt
      961 atgtccggcg cacctaccgg cccgggtggg aagtctagcg cggcgtctgt gctgccctga
     1021 tcgcggcccc acatccctca tagcctaggg g
//
LOCUS       DAOSPL010001625         1051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_144611_length_1051_cov_2.385542, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001625 DAOSPL010000000
VERSION     DAOSPL010001625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144611_length_1051_cov_2.385542"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="PLQ54_21855"
     CDS             <1..161
                     /locus_tag="PLQ54_21855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOS95968.1"
                     /translation="TLAIEPMVNMGSADVYTDDDGWAVRATDGKPSAHYEHTVAITER
                     GPVVLTVP"
     gene            203..469
                     /gene="infA"
                     /locus_tag="PLQ54_21860"
     CDS             203..469
                     /gene="infA"
                     /locus_tag="PLQ54_21860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOS95969.1"
                     /translation="MRPREQKEPDKEKAIRVMGTVTEKLPNAVFRVELENGHEVIAHV
                     SGKIRMRFIRIMPGDKVTLEVSPYDLTRGRITWLHRERTDRPPG"
     gene            566..682
                     /gene="rpmJ"
                     /locus_tag="PLQ54_21865"
     CDS             566..682
                     /gene="rpmJ"
                     /locus_tag="PLQ54_21865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002344960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOS95970.1"
                     /translation="MKVRASVKRMCDECKVIRRKGVVRVICPKHPKHKQRQG"
     gene            748..>1051
                     /gene="rpsM"
                     /locus_tag="PLQ54_21870"
     CDS             748..>1051
                     /gene="rpsM"
                     /locus_tag="PLQ54_21870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOS95971.1"
                     /translation="MARIAGIDIPRDKRVEVALTYIYGVGRPRSLQVLKQLEIDPNTK
                     VRDLTEEEVNRLRETLERSFTLEGDLRREVTGNIKRLMEIGCYRGLRHRRGLPVRGQ"
BASE COUNT          218 a          306 c          376 g          151 t
ORIGIN      
        1 tgacgctggc catcgagccc atggtgaaca tgggttcggc ggatgtgtac acggatgacg
       61 atggctgggc ggtgcgggcg actgacggaa agccgtcggc ccactacgaa cacaccgtgg
      121 ccatcacgga gcggggaccg gtggtcctca cggtgcccta gtccagtcgc cgggcgaccg
      181 agagaagagg caagcggggc ggatgcggcc cagagagcag aaagagccag ataaggaaaa
      241 ggcaatacgc gtcatgggca cggtcactga gaagttgccc aacgccgtat tccgggtgga
      301 gttggagaac ggccacgagg tcatcgcgca cgtctccggc aagattcgaa tgcggttcat
      361 ccggatcatg ccgggcgaca aggtgacgct ggaggtcagc ccgtacgacc tgactcgcgg
      421 ccgcatcacc tggctgcacc gcgagcgaac cgaccgtccg cctgggtaac accgggaggc
      481 ctcgagccga gccatggtgt ctggactcga ggcaaatggt cggcgcttgc ctgcctgttg
      541 cactgcagtc tggaggtaac tcaggatgaa ggtacgtgcc tcagtgaaga ggatgtgcga
      601 cgagtgcaag gtcatccggc gcaagggtgt ggtccgcgtg atctgcccca agcacccgaa
      661 gcacaagcag cgccagggct gaatggcgac acggggcccg ggcggtcgta ggcggccggc
      721 tggccttagg gggaggaacc cacatacatg gcacgcatcg ctggcataga catcccgcgg
      781 gacaagcggg tcgaggtggc gctgacctac atctatggag tgggccggcc ccgtagcctg
      841 caggtcctca agcagctcga gatcgacccg aacacgaaag tccgcgacct aactgaggag
      901 gaagtgaacc ggctccgcga gacgctggag cggagcttca ccctcgaagg cgatctgcgg
      961 cgcgaggtca cggggaacat caaacgcctg atggagatcg gctgctaccg ggggcttcgg
     1021 caccgccggg gtctgccggt ccggggccag c
//
LOCUS       DAOSPL010001626         1044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_146151_length_1044_cov_2.600607, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001626 DAOSPL010000000
VERSION     DAOSPL010001626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146151_length_1044_cov_2.600607"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PLQ54_21875"
     CDS             <1..183
                     /locus_tag="PLQ54_21875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95972.1"
                     /translation="GVILRPEDWDRLITWIDLNVPYAGDWSDTAQAPPESLIARRKEL
                     RDQDAQTRAAMAEAGR"
     gene            180..>1044
                     /locus_tag="PLQ54_21880"
     CDS             180..>1044
                     /locus_tag="PLQ54_21880"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOS95973.1"
                     /translation="MRALRAGLCAVCLGAVGVAPSFAQAQSWPFDAAQAHALQQAAAA
                     ELGVAAQRTVDLPGGLTLSLVLVPAGEFHMGAPADESPLDPDESPPVRVSIAAPFWMA
                     RCEVTNQQYRVFDPAHDSRFMDTYWKDRVGPGPSLNGDTQPVVRVSWLQAVDFCRWLA
                     QTTGAAFRLPTEAEWEWACRAGTDTPWSCPGEELFRFANYADATLQTIKPWALRDPDH
                     SDGQAVTAPVGQFEPNAWGLRDMHGNVAEWTSSVYLPYPYAADNAHESPEAAGPRVVR
                     GGSFDDRPRRVR"
BASE COUNT          142 a          376 c          367 g          159 t
ORIGIN      
        1 ggagtgatcc tcaggccgga ggactgggat cgcctcatca cctggatcga cctgaacgtg
       61 ccctatgccg gcgactggtc cgataccgcc caggcgccgc cggagtcgct catcgcccgg
      121 cgcaaggagc tgcgcgacca ggatgcgcag acgcgggccg ccatggcgga ggcgggccga
      181 tgagggctct ccgtgctggg ctatgtgctg tgtgtctcgg cgctgtgggt gtcgcgccca
      241 gctttgccca ggctcagtcc tggccgttcg acgcggcgca ggctcatgcg ctccagcagg
      301 ctgccgccgc ggagctcggc gtcgccgccc agcgcaccgt cgacctgccc ggcgggctga
      361 cgctctcgct cgttctagtg cccgccggcg agttccacat gggcgcgccg gccgacgagt
      421 cgccgctcga ccccgatgag tcgccaccgg tgcgagtgtc gatagcggcc cctttctgga
      481 tggcgcgctg cgaggtcacg aaccagcagt accgggtgtt cgaccccgcc cacgacagcc
      541 gtttcatgga cacctactgg aaggaccgcg tcggtcccgg gccctcgctc aacggcgaca
      601 cgcagcccgt cgtgcgcgtc tcgtggttgc aggccgtcga cttctgcagg tggctggctc
      661 agaccaccgg cgcggcattt cgcctgccga ccgaggccga atgggagtgg gcgtgccggg
      721 ccggcaccga cacgccttgg tcgtgccccg gcgaggagct cttccgcttc gccaactacg
      781 ccgatgcgac gctgcagacg atcaagccct gggctctgcg cgatcccgac cacagcgacg
      841 ggcaggccgt gaccgcgcct gtgggccagt tcgagccgaa cgcgtggggc ctgcgggaca
      901 tgcacggcaa cgtggcggag tggacctcca gcgtgtatct cccctacccg tacgcggccg
      961 acaatgccca cgagagcccc gaggccgccg ggccgagggt cgtccgcgga gggtcgttcg
     1021 acgaccgccc gcgtcgcgtg cgct
//
LOCUS       DAOSPL010001627         1043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_146326_length_1043_cov_2.994939, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001627 DAOSPL010000000
VERSION     DAOSPL010001627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146326_length_1043_cov_2.994939"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..607)
                     /locus_tag="PLQ54_21885"
     CDS             complement(224..607)
                     /locus_tag="PLQ54_21885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOS95974.1"
                     /translation="MEISVAPATTHPGVTVVALSGEIDGTGAQELGARLVREFELGHN
                     RIVLDMSGVSYLNSAGIGELAMAAKRARSLQGDLALCGLQDRVRRVLELVRMDQLLVV
                     ASGLEPACAALADHPVERDCGSSSA"
     gene            complement(710..>1043)
                     /locus_tag="PLQ54_21890"
     CDS             complement(710..>1043)
                     /locus_tag="PLQ54_21890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95975.1"
                     /translation="RPSPRQASRPAMTWLRMTRQRPRPPLAAHSANTPDGAPGPGSPR
                     NRVQRPMAEEFTHPQHVFGTELTVQESREYRAAPGVRRVPGVGVRATNRAQLEAILQD
                     SAPVLAQE"
BASE COUNT          167 a          371 c          347 g          158 t
ORIGIN      
        1 gtgccccagg gcatctcccc cggaatgtgc cgatcggccc agacgccgac ggccgtcccg
       61 agcgcgatgc agaccaccat ctgtacgccg atggacaaac cgcgggctga ctcccccagt
      121 tcaccctccc gccggcgggg ccgcggcggc gggggagcct cacggccgct cggcggttca
      181 tctgacggca tgggagctca gactcctcgg cgcgggcgtc acctcaggcc gagctggagc
      241 cgcagtcccg ctcaaccggg tgatcggcga gagcggcaca cgcgggttcg aggcccgagg
      301 cgaccaccag caactggtcc atgcgcacga gctcaagcac acggcggacg cgatcctgaa
      361 gaccgcacag tgccaggtcg ccctggaggc tgcgggctct cttggcggcc atggccagct
      421 caccgatgcc cgcactgttg agataggata caccagacat gtccaggacg atgcgattgt
      481 gcccgagctc aaactcgcgc acaagccggg cacccagctc ctgcgcaccg gtgccatcga
      541 tctcccctga cagagcgacg accgtgacac ccgggtgcgt ggtggcgggc gccacactga
      601 tctccaaagc cgatcactcc ccagtcggac gctcgttgcc gtggagcccc cgtggcatgg
      661 agtccgttcg cgatcctcac cgcggaagcc tccatggaac cctagcctat cactcctgtg
      721 ccagaactgg ggcggaatcc tgcagaatcg cctcgagctg ggcgcggttg gtcgcccgga
      781 caccgacgcc gggcacgcgg cgaacgcccg gagcagcccg gtactcccgg ctctcctgga
      841 ccgtcagctc tgtcccgaag acatgctgtg ggtgcgtgaa ctcttctgcc atgggccgct
      901 gaacgcgatt tcgcggtgag ccaggtcctg gcgccccgtc tggcgtattg gcgctgtggg
      961 ccgccagggg tggcctcggt cgctggcgag tcatgcgcag ccaggtcatt gccgggcggc
     1021 ttgcttgccg aggcgatggc cgg
//
LOCUS       DAOSPL010001628         1041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_146776_length_1041_cov_2.959432, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001628 DAOSPL010000000
VERSION     DAOSPL010001628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146776_length_1041_cov_2.959432"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PLQ54_21895"
     CDS             <1..768
                     /locus_tag="PLQ54_21895"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOS95976.1"
                     /translation="PPVVNVLLWQKLKAGTLGDAGAFSFCQSKTFTTGGEGGCVVTDD
                     EDLAWVCRSFRDHGYDVSTRMALLELEAKLPYIHNSIGFNFRMTEMQSAIGLKQLEVL
                     DSWNLARRRANGEYLLSALSGIPQIRKLPVHNDVVRNGFWLFPIILDIDALSCDIKQF
                     ADALTAEGIPNGPVMWPQCYQEKCYSEHRGFGRLRYPFRDPATRPEAVDYAHTYCPNA
                     AWVEKRCFFVPTHPTYELSHMELIAEGIKKVIAAFAK"
     gene            838..>1041
                     /locus_tag="PLQ54_21900"
     CDS             838..>1041
                     /locus_tag="PLQ54_21900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015148375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HOS95977.1"
                     /translation="MTLVEALLERLRTLPPEKQREVLDFAEFLAQQSTPRRPKRSLEG
                     LCADLDIHITEEDIAEARREMWGG"
BASE COUNT          194 a          329 c          336 g          182 t
ORIGIN      
        1 cccccggtgg tgaacgtttt gctctggcag aagctgaagg cgggcacact gggcgacgcc
       61 ggtgccttca gcttctgcca gagcaaaacg ttcaccaccg ggggcgaggg gggatgcgtg
      121 gtcacggatg atgaggatct ggcctgggtc tgccggtcct ttcgcgatca cggctacgac
      181 gtgagcaccc gcatggcgct gctcgagctt gaggccaagc tgccttacat ccacaacagc
      241 atcggcttca acttccgcat gaccgagatg cagtccgcca ttggcctcaa gcagcttgag
      301 gtgctcgact cgtggaatct ggctcgccgg cgcgccaatg gcgagtacct gctctcggcc
      361 ctgtccggga tcccacagat ccggaagctg cccgtgcaca acgatgtcgt gcggaacggc
      421 ttctggctgt tccccatcat cctcgacatc gacgcactga gctgcgacat caagcagttc
      481 gccgatgcac tgaccgccga gggcatcccc aacggccccg tcatgtggcc tcagtgctac
      541 caggagaagt gctactcgga gcatcggggc ttcggccgtc tgaggtatcc gttccgggat
      601 ccggccaccc ggcccgaggc tgtggactac gcccacacct actgcccgaa cgcggcgtgg
      661 gtggagaagc gttgcttctt cgtcccgact cacccgacgt atgagctctc gcacatggaa
      721 ctcatcgctg aggggatcaa gaaggtcatc gcggcgtttg cgaagtaggg gcgttggcga
      781 cgcagacccc caacggcgcg ggtacaatca ggtgtacgag acccaggggg tgatcccgtg
      841 actctggtag aggcgctgct cgagaggctg cggacgttgc cgcccgagaa gcagcgggaa
      901 gtgctggatt tcgccgagtt cctggcccaa cagtctaccc cgaggcgacc caaacggagc
      961 ctcgaagggc tatgtgcgga cctcgacatc cacatcaccg aggaggacat cgccgaggca
     1021 cgccgcgaga tgtggggcgg c
//
LOCUS       DAOSPL010001629         1039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_147251_length_1039_cov_2.976626, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001629 DAOSPL010000000
VERSION     DAOSPL010001629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147251_length_1039_cov_2.976626"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..>1039)
                     /locus_tag="PLQ54_21905"
     CDS             complement(205..>1039)
                     /locus_tag="PLQ54_21905"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HOS95978.1"
                     /translation="ELHPDFAEDAPATVAWAQRYFTLCPEKFIIKVPLTPAGFVAVRR
                     IAGLGIPVNYTLGFSARQNVLATLFSRPAFVNVFLGRLNAVVKDNGLGDGANVGEKAC
                     LASDAWVKRLRSEDGGPPTRQIAASMRGPDQVVTLAGVDVHTIPPKVFRGFVELGAAA
                     TIAPYSAVDLPVQADLDLSVLWGVPDAFVDFARRAHAEGGTIASGADLVRLAVESGFG
                     DLFHTWTDDERAEVRADGKIPVLSKWAGRVAIDDLMTRAALESFRVDQAALDDRLRGL
                     L"
BASE COUNT          178 a          367 c          347 g          147 t
ORIGIN      
        1 gggggcacgt acgtcggcac gtatgaggca ttcgaggcgg actcgatccc gagtggcctc
       61 ctcgggccgg tgcgactgct cgcggggggc gagtagacca acgcccggcg cctccggagc
      121 tgcgcgccag accgacccgc tcgtagctcg cggcctctgc gccgcggggt cggccgcgag
      181 gccgacagat gcctcgtcgc tcggctacaa cagccccctc agccgatcat ccagcgccgc
      241 ctggtccaca cggaacgact cgagcgccgc gcgggtcatg aggtcatcga tggcgacccg
      301 gccggcccac ttgctgagga cggggatctt cccatcggcg cggacctcgg cacgctcgtc
      361 gtcggtccag gtgtggaaca ggtcgccgaa accgctctca accgcgagcc ggaccagatc
      421 ggccccgctc gcgatcgtgc ccccttcggc atgcgcccgt cgcgcgaagt cgacgaaggc
      481 atccggcact ccccacagga cgctcaagtc caggtcggcc tggaccggca gatccacggc
      541 tgagtagggc gcgatggtgg cggcggcgcc gagctcgacg aagccgcgga acaccttggg
      601 aggaatggtg tgcacatcga cgccggccag agtgaccacc tggtccggtc ccctcatgct
      661 ggccgcgatc tgccgcgtgg gtgggccgcc gtcctcggag cggagccgct tcacccaagc
      721 atccgaggcc agacaggcct tctcgcccac attagcgcca tcgcccagcc cgttgtcctt
      781 gaccaccgcg ttgaggcgcc ccaggaacac gttcacgaag gcgggcctcg agaacaacgt
      841 cgcaagcacg ttctgccgcg ccgagaaccc cagcgtgtag ttgaccggga taccaagccc
      901 ggcaatccgc cgcaccgcga caaaccctgc gggagttaga ggcaccttga taataaactt
      961 ctccgggcag agggtgaagt agcgttgggc ccaggccacg gtggcgggtg catcttccgc
     1021 aaagtcgggg tgaagctcg
//
LOCUS       DAOSPL010001630         1039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_147253_length_1039_cov_2.896341, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001630 DAOSPL010000000
VERSION     DAOSPL010001630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147253_length_1039_cov_2.896341"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..>1039
                     /locus_tag="PLQ54_21910"
     CDS             83..>1039
                     /locus_tag="PLQ54_21910"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOS95979.1"
                     /translation="MPLPAVIEDAGRIVVTQPVAPFGPQGTQWRVMLVARSSLIDERV
                     AASDFHRGFVLVAIVCSVLPLAAGILLIGFFLRAIPKLQEAARAVAAGDLSHRVAIHS
                     GDEMEDLAQDFNYMATRLAEYGQLERAIALEKLKDDLIHMIVHDLRTPLTGILGTLQT
                     IESCDYDVEVTRDLVPGARRASVTLMGLINDLLDINKMEAGAMELDVARVDTTAVVEE
                     AVDMLRPLAEDKGLKLRLLECEGLPPMEADADKLRRIFVNLIGNAIKFTPSGGEVIVR
                     AGGGYDGQALHFMVEDTGEGIPPEYLDRIFEKFGQVETRKAGR"
BASE COUNT          173 a          326 c          353 g          187 t
ORIGIN      
        1 ccgtgtgctc ggtgatctcg aatgacaccc gggcaccggc atcgccggcg agtttccgcc
       61 gcccacggtc gagcgcgtcc gaatgccgct gccggccgtg attgaggatg cggggcgcat
      121 cgtggtaacc cagcccgtcg cgccctttgg gccccagggc actcagtggc gcgtcatgct
      181 ggtggcccga tcgagtctca tcgacgagcg ggtcgcggct tcggacttcc atcggggttt
      241 cgtgctggtt gccatcgtat gctccgtgct tccgttggcc gcgggcatcc tgctgatcgg
      301 tttcttctta cgcgccattc cgaagctcca ggaggccgct cgggcggtgg ccgctggcga
      361 tctgtcgcac cgagtggcca tccactctgg cgacgagatg gaggatctgg ctcaggactt
      421 caactacatg gctacccgac tggccgagta cggacagctc gaacgggcca ttgctctcga
      481 gaagctcaag gacgatctca tccacatgat cgttcacgac ttgcgcacgc cgctgacggg
      541 catcctcggc acacttcaga cgattgagtc ctgcgattac gacgttgagg tcacccgcga
      601 cctggtccct ggcgctcgcc gcgcgagcgt gacgctgatg gggctgatca acgacctgct
      661 ggacatcaac aagatggaag ccggcgccat ggagctcgat gtggcccgcg tcgacacgac
      721 ggcggtggtt gaggaggcgg tcgacatgct ccgcccgttg gcggaggaca aggggctgaa
      781 gctaaggctg ctcgagtgcg aggggctgcc gcccatggaa gccgacgcgg acaagcttcg
      841 ccggatcttc gtgaacctca tcgggaacgc catcaagttc accccttcgg gcggcgaagt
      901 gatcgtgcgc gccggaggcg gctacgacgg ccaggcgctc cacttcatgg tggaggacac
      961 gggcgaggga atcccgcccg agtacctgga ccgcatcttc gagaagtttg gccaggtgga
     1021 gacgcgcaag gccgggcgg
//
LOCUS       DAOSPL010001631         1037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_147792_length_1037_cov_2.069246, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001631 DAOSPL010000000
VERSION     DAOSPL010001631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147792_length_1037_cov_2.069246"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="PLQ54_21915"
     CDS             complement(<1..760)
                     /locus_tag="PLQ54_21915"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HOS95980.1"
                     /translation="MALFAPVQESDITRAIIERSMKDLLAMTPTDVVIVGGGPSGLVA
                     ARDLASQGVRTVIFEANNYLGGGFWLGGYLFSHLTVRAPAHEMLAEVGVPYEEYAPGL
                     YTALAPHCVAKFIGAACDAGAIVIGMTMVDDVCVRQDNRVAGVVINWNPVRYLPKAIA
                     AIDPVSVECRLVIDCTGHDARVCQCLAHRGLLELKGMGGMWVNESEDAIVEQTGEVHP
                     GLIAAGMAVSTVYGVPRMGPTFGGMLLSGRRAAQL"
BASE COUNT          179 a          351 c          351 g          156 t
ORIGIN      
        1 cgagctgtgc ggcgcgccgg ccagagagca gcatgccgcc gaaggtcggc cccatccgag
       61 gcacgccgta cacggtagac acggccatgc cggccgcgat gagcccggga tggacctcac
      121 cggtctgctc gacgatggca tcctcggact cgttgaccca catgcctccc atgcccttga
      181 gctcgagcag cccgcggtgg gccaggcact ggcagacccg cgcgtcgtgc ccagtgcagt
      241 cgatgaccag ccggcactcg accgacacgg ggtcgatggc agcgatggcc ttgggcaggt
      301 accggaccgg attccagttg atgaccacgc cggccacgcg gttgtcctgg cggacgcaca
      361 cgtcgtcgac catggtcatg ccaatgacga tcgcgccggc atcgcacgcc gcaccgatga
      421 acttggccac acagtgcgga gccagcgccg tgtacaggcc cggggcgtac tcctcatacg
      481 ggacgcccac ctcggccagc atctcgtggg ccggggcgcg gaccgtgaga tggctgaaca
      541 ggtagccgcc gagccagaac ccaccgccga ggtagttgtt cgcctcgaag atcactgtgc
      601 gaacaccctg ggaggccaga tccctggccg ccaccaaccc gctgggtccg cccccgacaa
      661 tgaccacatc ggtgggagtc atcgccagca ggtccttcat cgagcgctcg atgatcgccc
      721 gagtgatatc actctcttgc accggggcaa aaagcgccat atagccatct ctccctggtc
      781 cgttccgtgg cgcaactgcg gagggtcatt atcggttagc cggcccagat tggctagggg
      841 cgggggcgca cccagtctcg atcgcacggc gcgcgaccga tggcggcacc cagggccggg
      901 gtggcgcggc caacggcagc ccataccgat gactacccat gtaacgacta agccgtctgg
      961 gcaggattcc gggaggtacc tggttggcgg gcggccgggg atgggtgttt ggggctgggg
     1021 cgggccgggt cccgtgt
//
LOCUS       DAOSPL010001632         1036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_147908_length_1036_cov_3.661570, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001632 DAOSPL010000000
VERSION     DAOSPL010001632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147908_length_1036_cov_3.661570"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..1031
                     /locus_tag="PLQ54_21920"
     CDS             255..1031
                     /locus_tag="PLQ54_21920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95981.1"
                     /translation="MTRLRIAVGVLALGAMLLGPGRALSEEEAPAAEAQPEGNKAGRL
                     LNQLVEAVLRTGGAEVLGRLTAAKTIEELQRALADSAEVVSAVSGSARKSLITPAWVQ
                     LEGPGCMGQWHRLRSAVASADLSQGIVRFSFSAPLFMQGGPTEVSAELDLLQLTIPDA
                     GGPTDSYTSIRVDQKPVAVVGGSYSLESPTPAWAGGLDALRRLHMAYELQIVCDDREG
                     PMELNQVYTLRIKSLPWWPAGGLVEGLADGVTVASGRRDQ"
BASE COUNT          159 a          327 c          375 g          175 t
ORIGIN      
        1 agcactgtgg actcctggat ctcacgacca tcaacctaaa gcgcaaaggt cattactact
       61 cgccgtagtc ccaccgcagt gtcgccgctc ccgagccccg cgtcttgcga tagaaggttt
      121 gcctcactct gctggcgaag cagcagttga ggattcgggg tcgggcgtgg ccgagtgtgt
      181 cgtcatgtac atggggcggg tctgccccat ccactggcac ctcgtgtgcc tgcgcgcttc
      241 gagaggagcc tgacatgaca cggttgcgga tcgcggtggg cgtgttggcc ctgggggcca
      301 tgctgttggg gcccgggcgg gcgctcagcg aggaagaggc tccggcggcg gaggcccagc
      361 cggaagggaa caaggcgggc cggctgctga accagctcgt cgaggccgtt ctgcggacgg
      421 gcggcgccga ggttttgggc cggctgacgg cggccaagac catcgaggaa ctccagaggg
      481 ctctggccga ctcggctgag gtggtgtcgg ccgtgagcgg cagcgctcgc aagtcgctga
      541 tcaccccggc gtgggtgcag ctcgaggggc cgggatgcat gggacagtgg caccggctcc
      601 ggtccgccgt ggcgagcgcg gacctcagcc agggcatcgt tcggttcagt ttctctgcgc
      661 cgctcttcat gcagggaggt ccgacggagg tctcggccga gctggacctg ctccagctca
      721 ccataccgga cgcgggtggg cccaccgatt cctacacgag tatccgcgtc gaccagaagc
      781 cggttgccgt ggtcggtggc tcctactcac tggagtcccc gacgcccgca tgggccggcg
      841 gccttgacgc actccgccga ctccacatgg cctacgagct tcagatcgtc tgcgacgacc
      901 gggaggggcc gatggagctc aaccaggtct acacgctgcg tatcaagagc ctgccctggt
      961 ggccggcggg aggcctggtc gaggggctag ccgacggggt caccgttgca tcgggccgac
     1021 gcgatcagtg agcctg
//
LOCUS       DAOSPL010001633         1033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_148626_length_1033_cov_3.475460, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001633 DAOSPL010000000
VERSION     DAOSPL010001633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148626_length_1033_cov_3.475460"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..>1033)
                     /locus_tag="PLQ54_21925"
     CDS             complement(149..>1033)
                     /locus_tag="PLQ54_21925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95982.1"
                     /translation="WAEVVAAAKSEAPGAVVCSPELCTSMWWWAAALEGRRLGIQGPP
                     WLSDEIPYAGDWPRGDRLENYRDVFDLMAVSYYGFDANSMEWKDVAANRPALSAAAEA
                     GLWLARTHARPRRWLWAEAGWGAPVKDRPPFHLERDLATLLFGMDHCAGALLWQAKDN
                     EGSTAGVFTSGGERAATFDLLRAASDVVLADAAFFAADHAKANADGFPVAGDGFAEAD
                     PAVLTRFTGHHVLLFSEAPVETSVTLSQTGGATLAEIPVGHGYSSPWPLEVTGEADGR
                     ITLRRIRPGLLYVLRVDR"
BASE COUNT          154 a          404 c          331 g          144 t
ORIGIN      
        1 cagatccgcc agatgggccg cgttgtcggc cgtcgggcac cggatcgtcg gggacatcag
       61 tcggccgcca gcacggcccc cacggactag ccgcacgggg gtgggtcccc ggtgccgcgc
      121 cgtggacccg cccccggctc cccttggctc accgatcgac tcgcaacaca tacagcagcc
      181 cgggccggat gcgccgcaag gtgatgcgtc cgtccgcctc gcccgtgacc tccagcggcc
      241 atggactgct gtagccgtgg cccaccggga tctccgcgag tgtggcaccg cccgtctggc
      301 tcagggtgac gctcgtctcg accggtgcct cgctgaacag cagtacgtgg tggccggtga
      361 accgcgtcag cacggccggg tcggcctccg caaacccgtc gccggctacg gggaacccat
      421 cggcgttagc cttcgcgtgg tccgcggcga agaacgccgc gtccgccagg accacatcag
      481 aggcggccct cagcaggtcg aaagttgccg cacgctcacc tcctgatgtg aacacgccgg
      541 ccgtcgagcc ctcgttgtcc ttggcctgcc acagcagcgc gcccgcgcag tggtccatac
      601 cgaacagcag cgtggccaga tcgcgctcga ggtggaacgg cggacggtcc ttcaccgggg
      661 ccccccaccc cgcctcggcc cacagccagc gtctcggccg agcatgcgtg cgcgccagcc
      721 acagtcccgc ctccgcggcg gcgctcagag ccggccggtt tgcggccacg tccttccact
      781 ccatgctgtt ggcatcgaac ccgtagtacg agacggccat caggtcgaac acatcgcggt
      841 agttctcgag ccggtcgccc cgtggccagt ccccggcata ggggatctcg tcggaaagcc
      901 atggcggccc ctggatcccc agtcggcgcc cctccagcgc agctgcccac caccacatgc
      961 tggtgcacag ctcgggcgag cacaccacag cgcccggcgc ctcggacttg gcggccgcca
     1021 cgacctcggc cca
//
LOCUS       DAOSPL010001634         1032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_148901_length_1032_cov_2.623337, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001634 DAOSPL010000000
VERSION     DAOSPL010001634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148901_length_1032_cov_2.623337"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PLQ54_21930"
     CDS             complement(<1..809)
                     /locus_tag="PLQ54_21930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95983.1"
                     /translation="MMLMVHIAGTIAWCAVMAAAEGPAGPVLRVPLAPRAVAIDGHIG
                     RAEWEGAAVVRRLWLPGNATALPVPTELLLQYDPANLYLAARCTESEQGYPKAFPRQP
                     TDLLNDDDAVQVVLGTADSFAVARDVLQMGGYANAQGQPVSSADHYYQFTANAVGARA
                     RTYNESPLERPLFDAEVSRNERGWSVEMRVPFASAGIGDPSGQPMFVNLFRFRPPEMA
                     GWHLPAFGGYAPMPFGELVLLPRGSEAERTAEEALPPRTRVDKPRDPQARL"
     gene            complement(806..>1032)
                     /locus_tag="PLQ54_21935"
     CDS             complement(806..>1032)
                     /locus_tag="PLQ54_21935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95984.1"
                     /translation="SAYLKPDGNVLIVAANLSEQPVRGWLRLRGTLSEATHALTGDTV
                     TVANGGIVDDFPVWSARLYLAKLHQRGGRR"
BASE COUNT          157 a          357 c          360 g          158 t
ORIGIN      
        1 tcgagccggg cctgcgggtc cctgggcttg tctacccttg tccgcggggg caaggcctcc
       61 tcggcagtcc gctcggcctc cgagccgcgg gggagcagca ccaactctcc gaacggcatg
      121 ggcgcatagc cgccgaaggc cggcaggtgc catcctgcca tctctggcgg gcggaagcgg
      181 aacaggttta cgaacatagg ctgccccgac ggatcgccga ttcccgctga ggcgaacggg
      241 acccgcatct cgacggacca gccgcgttca ttccggctca cctctgcgtc gaagagcggg
      301 cgctccagtg gactctcgtt gtaggtacgc gcccgggcgc caacggcatt cgcggtgaac
      361 tggtagtagt ggtcagccga ggacaccggc tggccttggg cgttggcgta gccccccatc
      421 tggaggacgt cgcgcgccac ggcgaagctg tcggccgtgc cgagcacgac ctgcacggca
      481 tcgtcatcat tgagcaggtc ggtgggctgc cgcgggaacg ccttggggta gccctgctcg
      541 ctctcggtgc accgggcggc cagatagagg ttggccggat cgtactgaag caggagctcc
      601 gttggcaccg gaagcgcggt agcattgccg gggagccaca gccgccgcac cacggcggcg
      661 ccttcccact cggcgcggcc gatatggccg tcgattgcca cggcccgggg cgccagcggc
      721 actcgtagca cgggaccggc tgggccctca gccgctgcca tgaccgcgca ccaggcgatc
      781 gtgcccgcga tgtgcaccat cagcatcacc tgcggccgcc tctctggtgg agcttggcca
      841 ggtagagccg ggctgaccac accgggaagt cgtccacgat gccaccgttg gccaccgtga
      901 cggtgtcgcc cgtgagagca tgggttgcct ccgacaacgt ccccctgagc cgcagccacc
      961 cccgcaccgg ctgctccgag aggttcgcgg cgacgatgag cacgttgccg tcgggcttga
     1021 ggtaggcgct ga
//
LOCUS       DAOSPL010001635         1031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_149084_length_1031_cov_4.363730, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001635 DAOSPL010000000
VERSION     DAOSPL010001635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149084_length_1031_cov_4.363730"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLQ54_21940"
     CDS             complement(<1..292)
                     /locus_tag="PLQ54_21940"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOS95985.1"
                     /translation="MNDLAQTGKRPHARERVLCVALNCGIDKVYDCRGFALTGIHQPA
                     RWLTCAGGKAVNQARMLHALGIDVLVTGFAGGFVGRATEDQLRCEGIPHDFER"
     gene            complement(299..673)
                     /locus_tag="PLQ54_21945"
     CDS             complement(299..673)
                     /locus_tag="PLQ54_21945"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOS95986.1"
                     /translation="MPDSATTAGPDPELDVDLELALDQIGGDRELLVEIAAIFLDSYP
                     DHVAELERNLADNDLPLAARTAHAIKGAVSNFGAPGAIAAALAVEDAAKTGDMAKTWA
                     AFARLKEALAVVHTALAPLAGR"
     gene            complement(705..>1031)
                     /locus_tag="PLQ54_21950"
     CDS             complement(705..>1031)
                     /locus_tag="PLQ54_21950"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOS95987.1"
                     /translation="WGQRAQWCDYSGAVDGTRVGIAIFDHPANPMFPTYWHVRNYGLM
                     TANPFGVSYFEEGSGKRGDWLLQANRTATFRYRLFVHGGSAADAAVSDRYHDYTNPPA
                     ARWATE"
BASE COUNT          165 a          350 c          344 g          172 t
ORIGIN      
        1 cgcgctcgaa gtcgtgcggg atgccttcac agcgaagttg gtcctcggtg gcccggccga
       61 cgaaaccgcc ggcgaagccg gtgacgagca catcgatccc gagcgcgtgg agcatccgcg
      121 cctggttcac ggccttgccc cccgcgcagg tgagccaacg cgcgggctga tggatgccgg
      181 tcagcgcgaa tccgcggcag tcgtagacct tgtcgatccc gcagttgagg gcaacacaca
      241 gcacgcgctc tcgcgcgtgc ggtctcttgc ccgtctgagc caggtcattc actcgctgtc
      301 atcgccccgc caggggagcc aacgccgtgt gcaccacggc gagtgcctcc ttgagccgcg
      361 cgaaagcggc ccacgtcttg gccatgtcgc cggtcttggc cgcgtcctcg acggccagag
      421 ccgcggcgat cgcaccgggc gccccgaagt tggagaccgc ccccttaatg gcgtgagctg
      481 tccgcgctgc caggggcagg tcgttgtccg ccaggttgcg ttcgagctcg gccacgtggt
      541 ccgggtagct gtcgaggaag atcgcggcaa tctcgaccag aagctcccgg tcgccaccga
      601 tctggtccag agccaactcg agatcgacgt cgagctcggg gtcggggccc gcagtcgttg
      661 cgctatctgg catcgcacaa gcgcctcctg gccgacggtc cgggctactc cgtggcccag
      721 cgggccgccg gtggattcgt gtagtcatgg taccggtcgc tgaccgccgc gtcggcagct
      781 gatccgccgt ggacgaacag ccggtagcga aacgtggccg tcctgttggc ctggagcaac
      841 cagtctcccc gtttgcctga accctcctcg aagtacgaga caccaaacgg gttggcggtc
      901 atcagcccgt agttccgaac atgccagtag gtcgggaaca tcgggttggc gggatggtcg
      961 aatatggcga tgccaacacg ggtgccatct acggcgcccg aatagtcgca ccactgggcg
     1021 cgctggcccc a
//
LOCUS       DAOSPL010001636         1031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_149108_length_1031_cov_3.143443, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001636 DAOSPL010000000
VERSION     DAOSPL010001636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149108_length_1031_cov_3.143443"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /gene="hisG"
                     /locus_tag="PLQ54_21955"
     CDS             complement(<1..416)
                     /gene="hisG"
                     /locus_tag="PLQ54_21955"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOS95988.1"
                     /translation="MNDEQRVLKIGLPAGSMQESTIELFRRAGFPVAVASRRSYAPST
                     GDPELEARFIRAQEMARYVEHGVFDCGLTGHDWVIETEADVVELCDLAYAKQGAGKAR
                     WVLVVPEESDIVSIEQLQGKRIATELVGVTKRLLAER"
     gene            complement(649..>1031)
                     /locus_tag="PLQ54_21960"
     CDS             complement(649..>1031)
                     /locus_tag="PLQ54_21960"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOS95989.1"
                     /translation="MRTTDPPHWLLRSTSTDGGHSWTEPTPTTLPNPDSAAALLRLAS
                     GAVLLAYNPSQCRRTPLALALSDDEGDTFPVEVALESGEGEFSYPAMAQDAEGVIHVV
                     YTWDRKAIKHVTVTEADLRAAAGA"
BASE COUNT          165 a          334 c          364 g          168 t
ORIGIN      
        1 cgttcggcga gcaggcgctt ggtgacgccc acgagctcgg tggcgatccg cttgccctgg
       61 agttgctcga tggagacgat gtcggactcc tcgggcacaa cgagcaccca ccgcgccttg
      121 ccggccccct gcttggcgta ggccaggtcg cagagctcga ccacatcggc ctcggtctcg
      181 atcacccagt cgtgtccggt tagaccacag tcgaacacgc catgttccac gtagcgagcc
      241 atttcctggg cgcggatgaa ccgggcttcg agctcgggat cgccggtgga gggcgcatag
      301 gaccggcgcg aggccaccgc cacggggaac cccgcgcgac ggaagagctc gatggtcgat
      361 tcctgcatgc tgcccgcggg cagaccgatc ttcaggacac gctgctcgtc gttcatgact
      421 cacctttcgc agccgcaggc tcggcaagac ggggactccg cgggcccatt atgcgggata
      481 cgccaagaga tgaacagcca tgggtgggcg tggagccgtg gggcagactc cggactccag
      541 ccgagcgcgg cctgcccctc cgccctggac ccatgcgccg ttccagcgcg gaggaggaag
      601 tatgggcagc cctgagggga gtgtcaacgc gagggcgagc tcgaccgcct atgctccggc
      661 cgccgccctc agatctgcct cggtgactgt cacatgcttg atggccttgc gatcccaggt
      721 gtagaccacg tggatgaccc cctcagcgtc ctgtgccatg gccgggtatg agaactcgcc
      781 ttcgccggac tccagcgcca cttcgacggg gaaggtgtcg ccctcgtcat cgctgagtgc
      841 cagggccagg ggagtgcggc ggcactggga cgggttgtag gccaggagta cggcgccact
      901 cgcgagccgg agcagggccg ccgcggagtc ggggtttggc agcgtggtcg gtgtgggctc
      961 ggtccacgag tgaccgccat cggtcgaggt ggacctcaac agccagtggg gcgggtcggt
     1021 ggtccgcatt a
//
LOCUS       DAOSPL010001637         1030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_149449_length_1030_cov_1.834872, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001637 DAOSPL010000000
VERSION     DAOSPL010001637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149449_length_1030_cov_1.834872"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PLQ54_21965"
     CDS             <1..615
                     /locus_tag="PLQ54_21965"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOS95990.1"
                     /translation="KTHGQTLLTGAIKNLFGGVAPRERIQIHGLGGFEGLSEAIVDAF
                     ACQRPQLTVMDAIVAMEGNGPTRGRPRPLGLVAASGDAVALDAVMEHLAGFEPGEVRV
                     TAAAGRQGLGECNLTRVQVDGYDIRRAHVPLERPPGLIRHGMPRWVGRVVSRHLWVRP
                     AFDAARCVGCGACAAICPGGAIDMNGTARVNYDRCIECFCCQEV"
     gene            complement(817..>1030)
                     /locus_tag="PLQ54_21970"
     CDS             complement(817..>1030)
                     /locus_tag="PLQ54_21970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOS95991.1"
                     /translation="GSAGGTGFDLAESGFSQIQYIRVDGLSGFAGGEIDGFSDVRATP
                     EPATACLLLSAGAGMVWRRRHRRANT"
BASE COUNT          167 a          356 c          357 g          150 t
ORIGIN      
        1 aagacccacg gccagacact ccttacggga gcaatcaaga atctgttcgg tggagtggca
       61 ccccgggagc gcatccagat ccacggcctc ggcggattcg agggcctcag cgaagccatc
      121 gtcgacgcct tcgcgtgcca gaggccgcag ctgaccgtga tggatgccat cgtggcgatg
      181 gaagggaacg gccccacgcg tgggaggccc cgcccgctcg ggctggtggc agcgtccggc
      241 gacgccgttg ccctcgacgc ggtcatggag cacctcgccg gtttcgagcc cggggaagtc
      301 cgagtgacgg ccgccgcagg ccgccagggc ctgggtgaat gcaacctcac tcgcgtccag
      361 gttgatggat acgacatccg gcgcgcgcac gtgcccttgg aacggccgcc gggcctgatc
      421 cggcatggta tgccgcgttg ggtagggcga gtcgtgtcgc ggcacctctg ggtccggccc
      481 gcctttgatg ccgcgcgttg cgttgggtgc ggcgcctgcg ccgccatctg cccgggcggc
      541 gccatcgata tgaacggcac ggcccgggtc aactacgatc gctgcatcga gtgcttctgc
      601 tgccaggagg tctgaccggc cgacgccatc gactcgcgcg agagcctact ggcacgcgtg
      661 gtggtcggta gcagccggcg tgtgaggggg cgaggatcgg cagacccgag gcgctagacc
      721 gcagtctgcc agccaatggc ccaccaggag cgggagccgc cgccactccc gcactggaag
      781 cggcaggcgg ccccccgcac tcctgcggaa gccaggctag gtgttcgctc tccgatggcg
      841 ccgccgccat accattcccg cgccggccga aagaagcagg caggcggtgg ccggttcggg
      901 cgtggccctc acatcagaga acccatcgat ctcgccaccg gcgaaacccg atagcccatc
      961 gactcggatg tactggatct ggctgaagcc cgactcggcc agatcgaagc cagtccctcc
     1021 ggccgagccc
//
LOCUS       DAOSPL010001638         1026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_150247_length_1026_cov_3.354274, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001638 DAOSPL010000000
VERSION     DAOSPL010001638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150247_length_1026_cov_3.354274"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLQ54_21975"
     CDS             complement(<1..182)
                     /locus_tag="PLQ54_21975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95992.1"
                     /translation="MESAARAVVSGAKTLLAVASVALAAALSLAHALGPNRLAAMTVL
                     PVWTWVAPGIALGLLS"
     gene            455..967
                     /locus_tag="PLQ54_21980"
     CDS             455..967
                     /locus_tag="PLQ54_21980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOS95993.1"
                     /translation="MPSDRPDGLQVLWAPWRMDFIRSAKPAECIFCQKPAEANDAANL
                     LLYRGTECFVLMNLYPYTNGHLMVAPYRHVAMLPDLTASERAEMMDLTSLCVEVLQAS
                     VYPDGLNCGMNLGRAAGAGIDQHLHMHVVPRWVGDVNFMSTISSSKVIVQHLGETYAE
                     LKPEFDRRRR"
BASE COUNT          185 a          319 c          366 g          156 t
ORIGIN      
        1 tggctgagta ggccgagagc gatgcccggc gcgacccaag tccagaccgg caacacggtc
       61 atggcggcca gccggttcgg acccagtgca tgggcgagac tgagcgcggc cgcaagggcc
      121 acactggcca cggcaaggag ggtcttcgcg ccgctcacaa ctgcccgggc cgcgctctcc
      181 attcgctcgg cctcatcacg cgagctcggg gcgagagggc gcgcatggcg gcgatcgcgg
      241 cttggctgcc gggcgggtcg gctgcgcaac gaggtagaca gcggagggcc tccggtgacc
      301 aggatatgag ttgggagagg atacgcggga gggcgcgccg ggggcaaggg agagccgaga
      361 catgaaggag accgcacggc ccgggcgcaa gtaacagacg gcgaggcagc ctcgagggcc
      421 agcccctgcc gatcccgacg cgttgaggtg ctggatgcct tccgacagac ccgacggcct
      481 gcaggtcctg tgggctccct ggcgcatgga cttcatccgg tcggccaagc cggcggagtg
      541 catcttctgc cagaagcccg cagaggccaa cgatgcggcg aacctattgc tctaccgggg
      601 cacggagtgt tttgttctga tgaacctcta tccatatacc aatggtcact tgatggtcgc
      661 gccctaccgc catgtggcga tgctgcccga cctgacggct tccgaaaggg cggagatgat
      721 ggacctgacg tcactgtgcg tcgaggtcct gcaggcgagc gtgtacccgg atggcctgaa
      781 ctgcgggatg aacctgggac gggcggcagg agcggggatt gaccagcacc tccacatgca
      841 tgtcgtccct cggtgggtgg gcgatgtcaa cttcatgtcg accatctcgt cgtcgaaggt
      901 catcgtgcag catctgggcg agacctacgc ggagctgaag ccggagttcg accgccggcg
      961 gcgctagcga gggtcggccc gccactctcg cgagcactcg gggtgaaaag cactgccctc
     1021 caccca
//
LOCUS       DAOSPL010001639         1026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_150255_length_1026_cov_3.131823, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001639 DAOSPL010000000
VERSION     DAOSPL010001639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150255_length_1026_cov_3.131823"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..>1026
                     /locus_tag="PLQ54_21985"
     CDS             54..>1026
                     /locus_tag="PLQ54_21985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95994.1"
                     /translation="MPTSELTPDEASLRPLFAGAAATEGWTIGGRGIGRVPAPVAPGR
                     QGACLVLSTPSLSGWVTLDSPQVGPLPGDAACTAFWARADQDDVWLHFEWREHDQSRW
                     RTKVLLTRQWRLYAAPHGLLEYWKDNPSKGRGGPGDGFRAENADALAFGLTGESLQEG
                     RPYTVYVEGVSTGQDPLGEYLNLTLNSHFGAINMATFLEPPRDALSICDASAPLTDAI
                     GLAAVPGALPLPATWTLPARAPLGGFSATAQTAQGHAGAPLKARWAPLVRAVDRYGRT
                     RGTAFGVLYHFAGEYPGSSWAFSGIDNADLFEASSQAGRELFLALVAR"
BASE COUNT          127 a          362 c          354 g          183 t
ORIGIN      
        1 tctttggtgg ctatcccttc gaccggccgg tcttgcgaca gaatggcaca tgggtgccga
       61 cgagtgagct gacgcctgac gaggccagcc tccggccgct gttcgccggc gcggccgcca
      121 ccgagggctg gactatcggt ggacgcggca tcggtcgggt gccggctccc gtggcacccg
      181 gccgtcaggg cgcgtgcctc gtcctctcga ccccctcgct ctcgggatgg gtgacactgg
      241 actcgcccca ggtgggtccg ttgcccggtg atgccgcctg caccgcgttc tgggcccgtg
      301 ccgatcagga tgacgtgtgg cttcacttcg agtggcgcga gcatgaccag tcccgctggc
      361 gcaccaaggt gctcctgacg cgccaatggc ggctctacgc ggcgccccat ggcctgctcg
      421 agtactggaa ggacaacccc tcgaaagggc gtggcggccc gggggacgga ttccgcgctg
      481 agaacgccga tgccctggct ttcggtctga ctggcgagtc cctccaggag ggccgcccgt
      541 acaccgttta tgtcgagggg gtcagcaccg gtcaggatcc gctgggcgag tacctcaacc
      601 tgacgctgaa ctcgcacttt ggtgcgatca acatggccac gttcctcgaa ccgccgcgcg
      661 acgccctgtc gatctgcgat gcctcggcgc cgctcaccga cgccatcggt ctggcggccg
      721 tgccgggcgc cttgcccctc ccggcgacct ggacactgcc ggcgcgtgcc cccttgggcg
      781 ggttctcggc gaccgcccag accgcccagg gtcatgcggg tgcgcccctg aaggcccgtt
      841 gggctccgct ggttcgcgcc gtggaccgct acggccggac ccgggggact gcgtttggcg
      901 ttctctacca cttcgccggt gagtatccgg gatcgtcgtg ggccttctcg ggcatcgaca
      961 atgccgatct ctttgaggcc agcagccagg cggggcggga gctgttcctg gccctggtcg
     1021 cgcgcc
//
LOCUS       DAOSPL010001640         1026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_150285_length_1026_cov_2.644696, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001640 DAOSPL010000000
VERSION     DAOSPL010001640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150285_length_1026_cov_2.644696"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PLQ54_21990"
     CDS             <1..709
                     /locus_tag="PLQ54_21990"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOS95995.1"
                     /translation="HLWNSSRDAYVDCLRQDGSQSPVLSQQTNTVCYLCGVVPESRRA
                     AVEAVIREAPEGFVRIGSPFMMFFAFEALAELGEHQRIVDWTRQWWGLMLDCGATSCW
                     EVFPGFDPRWYTRSHCHAWSAGPGYFLPTYQLGVRPDAPGFTRARIAPIPADLSWARG
                     RVPTSAGEIEVDWRRREGSFRIDVSLPPGTEATLVLPFGGEPRVRPTGFGAVHVSGKW
                     HIELPAGGSCTAEQPDG"
     gene            798..>1026
                     /locus_tag="PLQ54_21995"
     CDS             798..>1026
                     /locus_tag="PLQ54_21995"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOS95996.1"
                     /translation="MNGSNPEPRGVVFALAAHPDDIDMMMAGTMLRLRSVGYELHYMT
                     IGNGSCGTAVHTKDEIIRIRTAEARAAAHMIG"
BASE COUNT          152 a          337 c          367 g          170 t
ORIGIN      
        1 gcacctgtgg aacagctcgc gcgacgccta tgtggactgc ctgcgccagg atggctccca
       61 gagcccggtg ctgagccaac agacgaacac cgtgtgctac ctgtgtggcg tggtacccga
      121 gtcgcgccgg gccgcggtcg aggccgtgat ccgcgaggcg ccggagggct tcgtgcggat
      181 cgggtcgccg ttcatgatgt tcttcgcatt cgaggctctg gccgaactcg gcgagcatca
      241 gcgcatcgtc gactggacgc gccagtggtg gggactgatg ctcgactgcg gcgccaccag
      301 ctgctgggag gtgttccccg gcttcgaccc gcgttggtat acgcggagcc actgccacgc
      361 atggtcggcc ggtccggggt acttcctgcc cacatatcag ctcggggtcc gtcccgatgc
      421 cccgggtttc acccgcgcgc gtatcgcgcc gattccggcc gacctctcat gggctcgcgg
      481 ccgcgtgccg acctctgccg gcgagattga ggtggactgg cgccgtcgag agggctcctt
      541 ccgcatcgat gtctctctgc cgccgggcac ggaggcgacg ctggtcctgc ccttcggcgg
      601 agagccacgg gtccgcccga cgggcttcgg ggcggtgcat gtatcgggca agtggcacat
      661 cgagctgccc gccggcggaa gctgcacggc ggagcagcct gacggctaat ggcgcgttcc
      721 gggcgcaggt cctttgccgc tggcctggcg aatgccacgc tcgcgcgcga catggccagg
      781 cagaatgggt ggagcaaatg aacggatcga atccggaacc ccggggcgtg gtgttcgcct
      841 tggcggcgca tccggacgac atcgacatga tgatggccgg caccatgctt cggctgcggt
      901 cggtgggcta cgagctgcac tacatgacca tcggcaacgg gagctgcggc acggcggtcc
      961 acacgaagga cgagatcatc cgcatccgca cggccgaggc gcgtgccgcg gcccacatga
     1021 tcggag
//
LOCUS       DAOSPL010001641         1020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_151778_length_1020_cov_2.361658, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001641 DAOSPL010000000
VERSION     DAOSPL010001641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151778_length_1020_cov_2.361658"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1020)
                     /locus_tag="PLQ54_22000"
     CDS             complement(<1..>1020)
                     /locus_tag="PLQ54_22000"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOS95997.1"
                     /translation="VTLAWIGAGDRGGRQLLAWDFLPLPEVQVVAICDPFRSRREAWA
                     ANVDEHYGQRGCRAYRDFREMLEREDVDGAVIATHDGWHVPAALACVRAGVDVYVEKP
                     LGVSVEQDLLLRDTARRYGAVFQYGTQQRSMSHCRHGCELVRNGRVGEITAIEVVAPE
                     GGAGGSTEPVPVPDDLDYDLWLGPCPWSPYTADRCINRGSFWVYDNSIGFLGGWGAHP
                     LDILDWALGDEHGIPVEYEGTGMIPIEGLYDTVATWEVHCRYADGLPLRFTSGGGDLT
                     QFIGTEGWIGISRGGLTAEPASLLSSRVGPHEVRLAESAHHARNFVQAVRTRVPAVSG
                     LESAVR"
BASE COUNT          176 a          369 c          326 g          149 t
ORIGIN      
        1 cgcacggccg actccaggcc gctcaccgcc ggcacgcggg tgcgaacagc ctggacgaag
       61 ttgcgcgcgt ggtgagcgct ttcggccagc cggacctcgt gggggccgac ccgcgaggag
      121 agaagcgagg ccggttcggc cgtgagccca ccacggctga taccgatcca cccctcggtg
      181 ccgatgaact gggtcaggtc gcccccaccg ctggtgaacc tcagtgggag gccgtcggca
      241 taccggcagt ggacctccca cgtggccacg gtgtcgtaga gcccctcgat cgggatcatg
      301 cccgtaccct catattccac cgggatgcca tgttcatcgc ccaaagccca gtcgaggatg
      361 tccagcgggt gggcgcccca tccgccgagg aagccgatgg agttgtcata cacccagaac
      421 gacccccggt tgatacaccg gtctgccgtg taggggctcc atgggcacgg gccgagccac
      481 aggtcgtagt cgaggtcatc gggcaccggg accggctcgg tggaacctcc ggcgccacct
      541 tcgggagcga cgacctcgat ggctgtgatc tcccccaccc tcccgttccg caccagctcg
      601 cacccatggc ggcagtgcga catggaccgc tgctgggttc catactggaa caccgctccg
      661 tagcgccggg cagtgtcccg gagaaggagg tcctgctcga cactgacgcc tagcggcttc
      721 tcgacgtaga catccacacc ggcacgaaca caggccagag ccgccggcac atgccagccg
      781 tcgtgcgtcg cgatcaccgc gccgtcgacg tcctcgcgct ccagcatctc gcggaagtcc
      841 cggtaggcgc ggcaaccccg ttggccgtaa tgctcatcga cgttcgccgc ccaggcctct
      901 cgccgcgagc ggaatgggtc gcatatggct acgacctgca cctcgggcaa cggcaggaag
      961 tcccaggcca gaagctggcg accaccgcgg tcaccggcgc cgatccaggc cagcgtcact
//
LOCUS       DAOSPL010001642         1019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_151940_length_1019_cov_3.185685, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001642 DAOSPL010000000
VERSION     DAOSPL010001642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151940_length_1019_cov_3.185685"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..928)
                     /locus_tag="PLQ54_22005"
     CDS             complement(140..928)
                     /locus_tag="PLQ54_22005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOS95998.1"
                     /translation="MRQEEHQALFEVEDTYWWFVGRRRILHTLIRHALDAAGTHPRRI
                     GDMGCGTGRNLMLLHEFGWAVGTDLSLPALRWCRRRGLQELVAAEITQLPFAEGSLDL
                     VAASEVLEHVQDDSAAAAELARIVRPGGLLLITVPAYQFLWSEHDEALDHFRRYSARH
                     TRRLLEEAGLRIERLSHCVSFLLAPICLFRVLQRALGRIRPRKTGRPKTAYIQMPGPI
                     SKLFVWLLAVEAWLMRYIDLPAGVSIVCLARKPGTPVEGRDKRA"
BASE COUNT          178 a          326 c          353 g          162 t
ORIGIN      
        1 gcctgtcgct gtggctgcgg agcatcccgg ccggttggtt ctacggggaa tgaggggcgg
       61 gggacttttg atccgaggct gaagcctcgg ctacgggact acgggacggg gtacgcgaga
      121 gccgggcggg atgcgctcgc tacgctcgct tatctcgccc ctccacgggc gtaccgggct
      181 tccgcgccag gcagacgatc gagacccccg ccggcaggtc gatataccgc atcagccatg
      241 cttcgacggc cagcagccat acgaagagct tcgagatggg gcccggcatc tggatgtagg
      301 ccgttttggg ccggccggtc tttctcggcc gaatgcgacc cagagcgcgc tgaagcacac
      361 ggaacaggca gatgggcgcc aggaggaagg acacgcagtg gctgagccgc tcgatccgca
      421 gcccagcctc ttccagcagt cgccgggtgt ggcgtgccga gtaacgccgg aagtggtcga
      481 gggcctcatc gtgctcgctc cacaggaact gataggcggg gaccgtgatc agcagcaggc
      541 ctcccggccg gacgatgcgg gcgagctcgg ccgccgccgc cgaatcgtcc tggacgtgtt
      601 ccagcacctc ggaggcggcg accagatcga ggctaccctc ggcgaagggt agctgcgtga
      661 tctcggcggc cacgagttcc tgaagtccgc gccggcggca ccatcgcagg gcggggagag
      721 atagatcggt gcccaccgcc cagccgaact cgtgaagcag catgagattc cggccggtgc
      781 cgcatcccat gtcaccgatg cggcgtggat gtgtgccggc ggcatcgagc gcgtgccgga
      841 tcagggtgtg gaggatgcgg cggcgcccca cgaaccacca gtaggtatcc tcgacctcga
      901 atagagcttg gtgctcttcc tgacgcatgg catccccagc gagacggtct cggtccccta
      961 ccccagcaac tcccgataga gcgccatatg ggcttcaacc atgcgttcca tggtatagc
//
LOCUS       DAOSPL010001643         1019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_152021_length_1019_cov_2.091286, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001643 DAOSPL010000000
VERSION     DAOSPL010001643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152021_length_1019_cov_2.091286"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PLQ54_22010"
     CDS             complement(<1..641)
                     /locus_tag="PLQ54_22010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS95999.1"
                     /translation="MVRLMVALTILGTTPPFPQNAAQPALMRLTLAAEAHIGPVYSPN
                     GQYIACIERELESERCLMLSRNGQRIAMGGCSGSQVAGLPSWSPDSRRICWPTTDGSL
                     WFLDLDGSSHETHLTAMGAPMQAIWQRRDARALIATPLGIYSFPIDSARPPWRLITAG
                     EPGQMGPISLSRDGSRLAYIAADGVYVTTGPASAGTPIVATPSPEMAYAGVWLA"
BASE COUNT          159 a          317 c          371 g          172 t
ORIGIN      
        1 gcgagccata ccccagcata ggccatctcg ggcgatggag tcgcaacgat gggggtgccc
       61 gcgctggccg gccccgtcgt cacgtagacc ccgtccgccg cgatgtacgc cagcctgcta
      121 ccatcgcgtg agagactgat cggccccatc tgaccgggct ctccggcagt gatgagccgc
      181 catggaggcc tggcggaatc gatggggaag ctatagatcc ccaacggtgt ggcgatcagg
      241 gcccgggcgt cgcgccgctg ccagatggcc tgcatcggcg cgcccatggc tgtaagatgc
      301 gtctcgtgcg agctgccatc gaggtcgagg aaccacagtg agccgtcggt ggtgggccag
      361 cagatgcgtc ggctgtccgg ggaccagctc gggagtccgg ccacctggga ccccgagcag
      421 ccccccatcg cgatgcgttg gccgttgcgg ctgagcatca ggcaacgctc ggactcaagt
      481 tcgcgctcga tgcaggcgat gtactggccg ttgggagagt agactggccc gatgtgggcc
      541 tcggcggcga gagtgagccg catgagcgcg ggctgggccg cgttctgcgg gaacggcgga
      601 gtggtgccca ggatggtgag cgccaccatc agcctgacca tgttgcggtg tcccccttct
      661 accactgaag ggtggtgcgg cggtgtgtgg ttcacgccgc ctggactcgg tggctgggct
      721 ggcgggcata gggcggtggt cgcatgaggc tactggggta cccactcgcc gagttggaag
      781 cgctcttgcg gggctggggt gaacccgcgt atcgcgcacg gcagcttttt gaatggttgc
      841 accgacacaa tgaactgcgg ttcagctgca tgacgagtct gccggggccg ctgcgggcac
      901 ggctcgaggc cgacttcggc acggatcgcc ccaccgtgct cgaccacacg ggcgacgacg
      961 atgcccgcaa gcttctcatc ggcctcgccg acggtgagcg agtcgagagt gtcctcatg
//
LOCUS       DAOSPL010001644         1018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_152160_length_1018_cov_2.937695, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001644 DAOSPL010000000
VERSION     DAOSPL010001644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152160_length_1018_cov_2.937695"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PLQ54_22015"
     CDS             <1..783
                     /locus_tag="PLQ54_22015"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOS96000.1"
                     /translation="VEGPGATVVSACASASHAIGVAFDLVRAGQADAIITGGTEATVT
                     PLALAGFSNMKALSTCNDTPQEACKPLDARRDGFVMAEGAGILVVESLEHAEKRGATI
                     EAELCGVGMSSDAYHITAPCPDGAGGRRAMEACLRVAGADPEEIDYINCHAPGTPAGD
                     ESEAIAIRALFGENARVAIGSTKSMHGHLLGAAGAVELIATIRSVQAGVILPTRNLRE
                     PDPACEGLDCVMDTVRSAPIRAALSNSFGFGGQNACAMVRRV"
     gene            827..>1018
                     /locus_tag="PLQ54_22020"
     CDS             827..>1018
                     /locus_tag="PLQ54_22020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS96001.1"
                     /translation="MDPFERGELEVRLGYRFTQPWLLDTAVRHSSYVQDGHDSPGESN
                     ERLEFLGDAILNFLVGEWLF"
BASE COUNT          152 a          320 c          371 g          175 t
ORIGIN      
        1 gtggaggggc ctggggcgac tgtcgtgtcg gcgtgcgcct cggcctcgca cgcgatcggc
       61 gtggctttcg atctggtccg cgcgggccag gccgacgcca ttatcaccgg ggggaccgag
      121 gcgacggtga cccctctggc gctggcgggc ttctcgaaca tgaaggccct ttcgacctgc
      181 aatgacacgc cccaagaggc gtgcaagcca ctggacgcgc gccgggacgg gtttgtgatg
      241 gccgagggcg cgggcatcct ggtggtcgag agcctcgagc acgccgagaa gcgcggagcg
      301 accatcgagg cggagctgtg cggtgtgggc atgagctccg acgcctacca tatcactgcc
      361 ccctgccccg acggggcggg gggtcggcgt gccatggaag cctgcttgcg ggtggccggc
      421 gcggaccccg aggagatcga ctacatcaac tgccatgctc cgggcacgcc cgccggagac
      481 gaatctgagg ccatcgcgat ccgcgcgctg ttcggcgaga acgctcgggt ggccatcggg
      541 tccaccaagt cgatgcacgg tcacctgctg ggagcggccg gagccgtgga gctcatcgcc
      601 acgatccggt cggtacaggc cggcgtgatc ctgccgacgc ggaacctgcg ggaacccgat
      661 cccgcatgtg agggacttga ctgcgtgatg gatacggtgc gctcggcccc gatccgcgcg
      721 gccctctcga actcctttgg gttcggtggc cagaacgcgt gtgcgatggt tcgtcgggtc
      781 tagctcggcg gttggagcac cccgccggtc tgttgggagc gggtgaatgg atccgttcga
      841 gcgtggggag ttggaggtcc gtctcggata ccgcttcacg cagccgtggc tcctggacac
      901 cgcggtgcgc cattcgtcct acgtgcagga cggacacgac agccccggcg agtccaacga
      961 acggctggag ttccttggcg atgccatcct caattttctg gtcggtgaat ggctattc
//
LOCUS       DAOSPL010001645         1016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_152638_length_1016_cov_3.176899, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001645 DAOSPL010000000
VERSION     DAOSPL010001645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152638_length_1016_cov_3.176899"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..>1016)
                     /locus_tag="PLQ54_22025"
     CDS             complement(251..>1016)
                     /locus_tag="PLQ54_22025"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOS96002.1"
                     /translation="AEQSGRPNEILGHIRSELDDEARLAARFRLPRVYLRVAAALAAL
                     LLSSGTLVSGGVLPWLRGVLTVYLPALAVLQAMVWTGRAFVRHPSMRDLRESLIALVP
                     GAGALQRERGLLRFARSIRALNRAGRSVPEAFALAVPVAAQRPLVAGLERGAAALIQG
                     ASLAQAFQATGLFDGHLVRLVAEGERTGRVDQIVSGLCQEQERRVATAQRTTFYVIWG
                     ALIALSLLVIGAGAVGGLVSLMAGLMRKILEFINTF"
BASE COUNT          158 a          354 c          368 g          136 t
ORIGIN      
        1 cggacggtct cgcccgcctg tgttcgcgcg acatagcgga acaggggcat cgggacacct
       61 ccagcgcatg gtgggcacat ggtgggccat gggcgcccgc ccgggccata gggtctcacc
      121 tcgggaccgc ccacgcaagc gccgagcggc gcccgggcca ttccgctctc caacgcgggc
      181 catcgttgcc tgagaggacg cccggcctgg gggggaacgg gacgagcctc ctggcgctgt
      241 gggttccgtg tcagaacgtg ttgatgaact ccaggatctt gcgcatgagg cccgccatga
      301 gcgacacgag cccgccgacg gcgccggcgc cgatcacgag caggctgagc gctatgagcg
      361 cgccccagat cacgtagaag gtcgtccgct gcgcggtggc gacgcgacgt tcctgctcct
      421 ggcagagccc actgacaatc tggtcgaccc tgccggtgcg ctcgccctcg gccacgagcc
      481 ggaccaggtg accgtcgaag agtcctgttg cctggaaggc ctgagccagt gacgcccctt
      541 ggatgagcgc cgcggcaccc cgttccaggc cggcgaccag tggccgttgt gccgcgacgg
      601 gcacggccag ggcgaatgcc tccggtacgc ttcgtccggc ccggttcagc gcgcggatgg
      661 accgggcgaa ccgcaggagg cctctctcgc gctggagggc gccggcgccg ggcaccaggg
      721 cgatgagcga ctcccgcaga tcgcgcatgg acggatgacg cacgaaggct ctgccggtcc
      781 acaccatggc ctggagcact gcgagcgccg ggagatagac ggtgagcacc ccccggagcc
      841 atggcagaac gcccccgctg actagtgttc cggagctcag cagtagtgcg gcgagggcgg
      901 ccgcgacgcg gaggtagacc cggggcaggc ggaagcgcgc cgccagccgc gcttcgtcgt
      961 ccagctcgga ccggatgtgc cccaggatct cgttgggccg accgctctgc tcggca
//
LOCUS       DAOSPL010001646         1013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_153387_length_1013_cov_3.855950, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001646 DAOSPL010000000
VERSION     DAOSPL010001646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153387_length_1013_cov_3.855950"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1013)
                     /locus_tag="PLQ54_22030"
     CDS             complement(<1..>1013)
                     /locus_tag="PLQ54_22030"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOS96003.1"
                     /translation="AASWDANTDSTVYTLHLRRGVRWSDGAPYTSEDFMFWWELCLDK
                     RHFFEPPYFTYSAGQVMRVGAPDPFTLRLTFARPNYFLPLDLASGYWWNEQYNIPKHY
                     MRQFHPDHNPAYTDFVVFNEKNIVHSNPDRPTLAPWHLATLDKAATRVIYERNPYYWA
                     VDTSGRQLPYIDRIETTYAQTAETLLLKAMAGELDCQFRNIDLEDAALLHQFAGKGGY
                     ELRRWESAAGTDPGVLINWTPPDPKMRDLFRDKRFRRALSHAINRQRINQVAWSGLGV
                     PQGGTVSRQAWHFASTKGRQVYDAWAKAHADYDPGKASAYLDDVGLSRRDSQGLRTWP
                     DGT"
BASE COUNT          172 a          304 c          347 g          190 t
ORIGIN      
        1 ggtgccatcg ggccacgtcc gtaggccctg gctatcgcgg cgcgaaagcc ccacatcatc
       61 gaggtacgca ctcgccttcc ccgggtcgta gtcggcatga gccttggccc aggcgtcgta
      121 gacctgccgg cccttcgtgc tcgcgaaatg ccatgcttgg cgtgagaccg tgccgccctg
      181 gggcacgccg agcccggacc aggcgacctg gttgatccgc tgccggttga tggcgtgcga
      241 gagtgcgcgg cggaagcgct tgtccctgaa caggtcgcgc atcttcgggt cagggggcgt
      301 ccagttgatc agcacaccgg gatcggtccc agccgccgac tcccaacgcc ggagctcgta
      361 cccgcccttg ccggcgaact ggtgcagcaa tgcggcatcc tcgagatcga tgttgcggaa
      421 ctggcagtcg agctcgcctg ccatcgcttt gagcagcagt gtctccgcgg tctgggcgta
      481 ggtcgtctcg atgcggtcaa tgtagggcag ctgccgcccc gacgtgtcga ccgcccagta
      541 gtaagggttc cgctcataga tgacacgcgt ggcggccttg tcgagtgtcg ccaagtgcca
      601 cggcgccaat gtcgggcggt cgggattgct gtgcacaatg ttcttctcgt tgaagaccac
      661 gaagtcggtg tacgcggggt tgtggtccgg atggaactgc ctcatgtagt gcttggggat
      721 gttgtactgc tcgttccacc agtagcccga agccaagtca agtgggagga agtagttcgg
      781 gcgggcgaaa gtgagccgga gcgtaaacgg gtcgggggcc ccaaccctca tgacctggcc
      841 agcggagtac gtgaaatagg gcggctcgaa gaagtgccgc ttgtcgaggc agagctccca
      901 ccagaacatg aagtcctctg aggtgtacgg ggcgccgtca gaccagcgca cgccgcgcct
      961 cagatggagt gtgtagaccg ttgagtctgt gttggcgtcc cacgaggccg cta
//
LOCUS       DAOSPL010001647         1013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_153431_length_1013_cov_2.674322, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001647 DAOSPL010000000
VERSION     DAOSPL010001647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153431_length_1013_cov_2.674322"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PLQ54_22035"
     CDS             <1..949
                     /locus_tag="PLQ54_22035"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOS96004.1"
                     /translation="ALAMFDRFSIPMPGFLQGLQSRQRLRSGYLGVFLSGFAFAFLAY
                     ACLAPVVGTIALVLFRGGRVLEGGLAMFCFGLGVGFPFVLLGTFTGLITAVMQRGSAM
                     EKVKHAFGWAMLVLAGVFVFQAGEQRAKDLAAAAVAVRPPPPAVAPATATPDIVSLAG
                     LPTISLDAKTSAGSAEVGQPACSFTWSDPTTGEVRALGDLLDTPVWLTFWADWCHNCP
                     EEVPLMNALSTRYAGRLRVIGINEGDAPAVAQAWMAKHGVRYDVLRDAEETVAKDGFG
                     LTGIPYNILIDRNGIIRFSSAGLPRDADRVINEVLGESR"
BASE COUNT          142 a          357 c          331 g          183 t
ORIGIN      
        1 cgcactggcc atgttcgacc gcttctccat ccccatgccc gggttcctgc aggggctgca
       61 gtcgaggcag cggctccgat ccggctacct gggcgtgttc ctctccggct tcgccttcgc
      121 gttcctcgcc tacgcctgcc tcgcacccgt cgtgggcacc atcgccctgg tcctgttccg
      181 gggcgggaga gtactcgagg gcgggcttgc catgttctgc ttcgggctcg gcgtgggctt
      241 cccgtttgtc ctgttgggca cgttcaccgg gctcatcact gccgtgatgc agcgcggcag
      301 cgcgatggag aaggtgaagc acgccttcgg ctgggccatg ctcgtcctcg ccggcgtgtt
      361 cgtcttccag gccggcgagc agcgagccaa ggatctggct gccgcggccg tagccgtccg
      421 gccgcctccc cccgcggttg cccctgcgac ggcaaccccg gacatcgtgt ccctcgccgg
      481 gctccccacc atctcgctgg atgccaagac ctcagcgggc agtgccgagg tcggacagcc
      541 cgcgtgctcg ttcacctggt ccgaccccac aacgggcgag gtgcgcgcgc tcggcgacct
      601 gctcgacacg cccgtctggc tcacattctg ggccgactgg tgccacaact gccccgagga
      661 agtcccgctg atgaacgcgc tgagcacccg ctacgccggc cggctccgtg tcatcggcat
      721 caacgaaggc gacgcccctg ccgttgctca ggcctggatg gccaagcacg gcgttcgata
      781 cgacgtattg cgcgacgcag aggagacggt ggccaaagac ggctttggcc tgaccggcat
      841 cccctataac atcctgatcg accgcaacgg aatcatccgc ttcagctcgg cgggactccc
      901 gcgagacgcg gaccgggtga tcaacgaggt gctgggcgag tcgcgctagc gcctcggcat
      961 tcttgggcag caggagacgg atgcgcaatg ggtgttgtgg atctggaccg tgg
//
LOCUS       DAOSPL010001648         1011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_153935_length_1011_cov_2.789749, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001648 DAOSPL010000000
VERSION     DAOSPL010001648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153935_length_1011_cov_2.789749"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PLQ54_22040"
     CDS             complement(<1..194)
                     /locus_tag="PLQ54_22040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS96005.1"
                     /translation="MNPRQRVRAAYTGEPVDRPPVAAPYVGLYHQDHFGELTGQPQWQ
                     VHAWLHSSPEDHVAALKRMV"
     gene            363..>1011
                     /locus_tag="PLQ54_22045"
     CDS             363..>1011
                     /locus_tag="PLQ54_22045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS96006.1"
                     /translation="MIPVAKRIGSHVVVLTLVGTLLAWTGCGKPAAPLTRTISSKGGA
                     SDSAPSAKAGAARVTERPARPREELFWGGSGTPGMPDSFGPAGPSPVLLFGSRVAAGE
                     MEFQGDETHGSVIAAQSTDTHLEYERDDDIDARWVELPPPSGDATQVQHFAGMAVWGT
                     VARSAQRTATDEYEAESAIYLTRMPYDQVVAQYTPSDSADPATGLAGKGWTKTDAA"
BASE COUNT          153 a          359 c          362 g          137 t
ORIGIN      
        1 tggaccatgc gtttgagcgc ggccacgtgg tcctccgggc tcgaatggag ccaggcgtga
       61 acctgccact gcggctgtcc ggtgagttcc ccgaagtggt cctgatggta caggcccaca
      121 tagggagccg ccaccggcgg ccggtccacc ggctcaccgg tgtaggcggc tcgcacccgc
      181 tgtcttgggt tcatgacccc acctcatgct ggccccaccg cgacccggcg agagggcagg
      241 actcgtcgcc gacgaaccgc gggatcaggg ccagcgcccg ccgccccggg gtcgaaccgg
      301 tggccgtgtc cggcccgttc ttggggcggt gcgggccgag tccggaatcc gggagtggcg
      361 aggtgatccc tgtggcgaag cgcattgggt ctcacgtggt agttctcaca ctggtgggca
      421 cactcctggc ctggaccggg tgcggcaagc cggcggcccc cctgacccgt accatcagca
      481 gcaagggcgg ggcgagcgat agcgcgccct cggccaaggc gggcgccgcc agagtgacgg
      541 aacgaccggc acggccgcgc gaggagctgt tctggggcgg cagcggcacc ccgggcatgc
      601 ccgactcctt cggcccggcg ggccccagcc ccgtgctgct gttcggatcg cgagtcgcgg
      661 cgggtgagat ggaattccag ggcgacgaga cccacggctc tgtgatcgcg gcccagtcca
      721 ccgacaccca cctcgagtac gagcgcgacg acgacatcga tgcccgctgg gtcgaactcc
      781 cgccgcccag cggcgacgcg actcaggtcc agcacttcgc cggcatggcg gtctggggca
      841 cggtggcccg gtcggcccag aggacggcca ccgatgagta cgaggccgag tcggccatct
      901 acctgacccg gatgccctac gaccaggtcg tggcccagta cacaccctcg gactccgcgg
      961 atccggccac cggcctggcc ggcaaggggt ggacgaagac cgatgccgct c
//
LOCUS       DAOSPL010001649         1004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_155680_length_1004_cov_3.014752, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001649 DAOSPL010000000
VERSION     DAOSPL010001649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155680_length_1004_cov_3.014752"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PLQ54_22050"
     CDS             <1..442
                     /locus_tag="PLQ54_22050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HOS96007.1"
                     /translation="IEAGSTAVFLCAEAFREGDDPVRWMPLSPKGAVVGLASWLYHKD
                     EWAKAHPIFEGLPAGGLMDYEVYAELIPDVGFNGQPLPTEAVAGGCNASQDYSSGLFV
                     AVYPLGKGRVILNSLRVRETLGGEPVAERLLRNMLRYAAGKTGE"
     gene            753..>1004
                     /locus_tag="PLQ54_22055"
     CDS             753..>1004
                     /locus_tag="PLQ54_22055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS96008.1"
                     /translation="MAPRWVLGSLLLCTAAAHPAEYRLPLLPAAPAVDGAIGEDEWTG
                     ALWIDGFAWQGALERRTATGIIGATATHLYLAIRSQLPAE"
BASE COUNT          151 a          349 c          352 g          152 t
ORIGIN      
        1 catcgaggcc ggctccaccg ccgtcttcct gtgcgcggag gcattccgcg agggcgatga
       61 cccggtccgc tggatgccgc tctcgcccaa gggcgccgtg gtcggcctgg cctcgtggct
      121 ctaccacaaa gacgagtggg ccaaggcgca cccgatcttc gagggcctgc ccgccggcgg
      181 gctgatggac tacgaggtct acgccgagct gatccccgat gtaggtttca acggccagcc
      241 actgccgacg gaggccgtgg ccgggggctg caacgcctca caggactact cctcggggct
      301 gttcgtcgcc gtctacccgc tgggcaaggg ccgggtgatc ctgaacagcc tgcgggttcg
      361 agagacactc ggtggcgagc ccgtggccga gcgcctgctg cgcaacatgc tgcggtacgc
      421 ggccgggaag acaggggagt agggcgagtg agccttgggc gtggaccggc ggccgaccta
      481 agccccgtcg gccctgagga gcgacctacc cgcagcggtc gagagcgcct cgaatgggta
      541 agacggggcc tgggcgtcgc cccacaggga tcttccggaa gacttcaaga ggatagcgcg
      601 gggcagtgtg gaacgatatc cgtacgacgc attggcgcga ccggccggta gtgtggcctc
      661 ctacatcggg ccggtcgcgt cgcgtacgcc acgcagggcc gcacatcggc accggggaag
      721 cctcctgagt tgcgcttcgg gaggtgccgc tcatggcccc gcgatgggtc ctcggatctc
      781 tgttgctctg caccgccgcc gctcaccccg ccgagtaccg cctgcccctg ctgccagccg
      841 cgcccgctgt cgacggcgcc attggcgagg acgagtggac cggagccctg tggatcgatg
      901 gcttcgcctg gcagggcgcg ctcgagcgcc gcacagcaac cggcatcatc ggcgccacgg
      961 ccactcatct ctacctggcc atccgatccc agctcccggc cgag
//
LOCUS       DAOSPL010001650         1003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_2320
            NODE_155950_length_1003_cov_2.564346, whole genome shotgun
            sequence.
ACCESSION   DAOSPL010001650 DAOSPL010000000
VERSION     DAOSPL010001650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864387
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 06:06:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,412
            CDSs (total)                      :: 4,389
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 4 of 47
            Pseudo Genes (incomplete)         :: 39 of 47
            Pseudo Genes (internal stop)      :: 4 of 47
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155950_length_1003_cov_2.564346"
                     /isolate="AMR_MDS_2320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(7..71)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            326..>1003
                     /locus_tag="PLQ54_22060"
     CDS             326..>1003
                     /locus_tag="PLQ54_22060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOS96009.1"
                     /translation="MLRVAICSLLCAGMLSTAAPAQDRAVTIGSGVTPLADIGIYRVA
                     YQSYGHELVEMPSSWVGHFDGATGISYVPHSLIAGRDALLLHSPWRTDPGLTRVEYRL
                     ALPDVRPLSLAFGIAMAPGMVGPNRSDGVTFSAAVIDEGAEHEFLRQHYDSSEWHDIA
                     FDLAPWAGKTVTVRLQVEPGPANNPSFDYSYFGDAKITAGEGGEDKRQLLRRLTETRA
                     YRATRDLP"
BASE COUNT          165 a          344 c          322 g          172 t
ORIGIN      
        1 ggcgatctgg ctcccgccgc tccaggggaa cggcggtcac agttgcggga cagccacgga
       61 tttgcaccgt gttccggcag agcacagctc tgtcgtccca tcggccgaca cgtgcgcccg
      121 ggcatcacac gcaccaggca gagcagcgcg atgcgctcgc tcccaacacg gtctgttacg
      181 ctattgtcac tagcatgtta gcgtatacgc ctgtcccctg cgatgtcaac gggctgtttg
      241 cctggaggca tggcggcagg cgcatgcgaa gacacacccg cgcctgggcg gggaccgccc
      301 gtcggggccc tgggagggct ttctcatgct gcgagtcgcc atatgctcac tgctgtgcgc
      361 ggggatgctg agcaccgccg cgccagctca agaccgcgcg gtcaccatcg gtagcggcgt
      421 gacgcccctg gccgacattg gcatctaccg agttgcctac cagtcgtatg gtcacgaact
      481 ggtcgagatg ccgagttcgt gggtcgggca cttcgatggc gccacgggca tctcatacgt
      541 gccacacagc ctcatcgcgg gacgggacgc gctactcttg cactccccat ggcgcacgga
      601 cccgggcctc acacgggtcg agtaccgcct ggctctgccc gacgtacggc cgctgagcct
      661 tgcctttggc attgccatgg ctcccggcat ggtgggtccc aaccgatctg atggtgtgac
      721 cttcagcgcg gcggtcatcg atgaaggcgc tgagcacgag ttcctccgcc agcactacga
      781 ctcgagtgag tggcacgata tcgccttcga cctggccccc tgggcgggga agaccgtcac
      841 cgtgcgcctc caggtcgagc cgggccccgc gaacaacccg tcgttcgact actcatactt
      901 tggcgacgcc aagatcacgg ccggggaggg gggcgaagac aagcgccagc tgcttcggcg
      961 cctgaccgag acccgggcct atcgtgccac gcgcgacctg cca
//
